FN Thomson Reuters Web of Science™ VR 1.0 PT J AU DUPONTVERSTEEGDEN, EE KATZ, MS MCCARTER, RJ AF DUPONTVERSTEEGDEN, EE KATZ, MS MCCARTER, RJ TI BENEFICIAL VERSUS ADVERSE-EFFECTS OF LONG-TERM USE OF CLENBUTEROL IN MDX MICE SO MUSCLE & NERVE LA English DT Article DE MDX MOUSE; MUSCULAR DYSTROPHY; CLENBUTEROL; VENTILATION; SKELETAL MUSCLE ID DUCHENNE MUSCULAR-DYSTROPHY; SKELETAL-MUSCLE; ELECTRICAL-STIMULATION; DENERVATED MUSCLES; BODY-COMPOSITION; MOUSE DIAPHRAGM; BETA-AGONIST; EXPRESSION; MEMBRANE; EXERCISE AB Long-term administration of the beta(2)-adrenergic agonist clenbuterol in mdx mice was used to test the hypothesis that increasing contractile protein content in skeletal muscle will decrease the progression of muscular dystrophy, C57BL/10SNJ (control) and dystrophic (mdx) mice were given clenbuterol (1.0-1.5 mg/kg body weight/day) in the drinking water, Ventilatory function and morphological and functional characteristics of soleus (SOL) and diaphragm (DIA) muscles were evaluated, Clenbuterol administration was associated with increased SOL muscle weight, and SOL muscle weight to body weight ratio in control and mdx mice at both ages, There was a 22% increase in myosin concentration of mdx DIA at 1 year of age, correlating well with increased normalized active tension in mdx DIA at this age, Also, absolute tetanic tension increased in control and mdx SOL with clenbuterol at both ages, Ventilatory function was significantly impaired in mdx mice at both ages and clenbuterol administration did not alleviate this, Clenbuterol treatment was associated with a 30-40% increase in fatigability in DIA and SOL muscles of control and mdx mice at both ages, Furthermore, 1-year-old mdx mice receiving clenbuterol exhibited deformities in hindlimbs and spine, These results suggest that long-term clenbuterol treatment has a positive effect on muscle growth and force generation, but has adverse side effects such as increased muscle fatigability and development of deformities, (C) 1995 John Wiley & Sons, Inc. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284. NR 55 TC 34 Z9 34 U1 0 U2 2 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-639X J9 MUSCLE NERVE JI Muscle Nerve PD DEC PY 1995 VL 18 IS 12 BP 1447 EP 1459 DI 10.1002/mus.880181216 PG 13 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA TF655 UT WOS:A1995TF65500013 PM 7477069 ER PT J AU Gupta, SR Mlcoch, AG Scolaro, C Moritz, T AF Gupta, SR Mlcoch, AG Scolaro, C Moritz, T TI Bromocriptine treatment of nonfluent aphasia SO NEUROLOGY LA English DT Article AB Using a double-blind, placebo-controlled, crossover design, we studied the effect of bromocriptine (15 mg daily) in 20 men with chronic nonfluent aphasia. The study was conducted over a 28-week period in two phases. In phase I, the patients received either bromocriptine or placebo; in phase II the treatments were crossed over. We evaluated each patient's language and nonverbal cognitive skills at the beginning and end of each phase and 6 weeks after completion of phase II. When compared with placebo treatment, bromocriptine did not significantly improve the patients' speech fluency, language content, overall degree of aphasia severity, or nonverbal cognitive abilities, Based on these results, bromocriptine is not recommended as monotherapy for the treatment of chronic nonfluent aphasia. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,AUDIOL & SPEECH PATHOL SERV,HINES,IL 60141. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,COOPERAT STUDIES PROGRAM COORDINATING CTR,HINES,IL 60141. RP Gupta, SR (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,NEUROL SERV 127,HINES,IL 60141, USA. NR 9 TC 44 Z9 45 U1 0 U2 2 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD DEC PY 1995 VL 45 IS 12 BP 2170 EP 2173 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA TL374 UT WOS:A1995TL37400008 PM 8848187 ER PT J AU Will, MJ Ester, MS Ramirez, SG Tiner, BD McAnear, JT Epstein, L AF Will, MJ Ester, MS Ramirez, SG Tiner, BD McAnear, JT Epstein, L TI Comparison of cephalometric analysis with ethnicity in obstructive sleep apnea syndrome SO SLEEP LA English DT Article DE cephalometric anaylsis; ethnicity; obstructive sleep apnea ID ROENTGENOGRAMS; ABNORMALITIES AB Many studies have documented significant craniomandibular abnormalities in obstructive sleep apnea syndrome (OSAS) patients. Recent literature clearly describes the cephalometric abnormalities commonly associated with OSAS. Studies have not evaluated specific cephalometric abnormalities that may contribute to OSAS by various ethnic groups. Data were collected on 48 patients (20 Caucasian, 15 Black and 13 Hispanic) with completed cephalometric analysis and polysomnography. Cephalometric landmarks, angles and measurements [angle measured from sella to nasion to subspinale point (SNA), angle measured from sella to nasion to supramentale point (SNB), difference between SNA and SNB (ANB), perpendicular distance from gonion to gnathion to hyoid (MP-H), distance from posterior nasal spine to tip of soft palate (PNS-P) and posterior airway space (PAS)] commonly used in the evaluation of OSAS patients were recorded. Measurements were normalized by dividing the observed value by the mean value for the ethnic group. Statistically significant differences in normalized SNA and SNB appeared in the Black and Hispanic groups when compared to the Caucasian group. For both SNA and SNB, Blacks averaged approximately 3.5% above their ethnic mean, whereas Hispanics averaged 1.8-2.8% below their ethnic mean. There was a statistically significant correlation between respiratory distress index (RDI) and MP-H. These baseline cephalometric differences in the ethnic groups studied suggest that surgical intervention might be approached differently in various ethnic groups. Further studies that evaluate the surgical success achieved by various procedures among different ethnic groups may help define surgical protocol in various ethnic groups for OSAS. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT & SLEEP DISORDERS,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT ORAL & MAXILLOFACIAL SURG,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,DEPT ORAL & MAXILLOFACIAL SURG,SAN ANTONIO,TX 78284. WILFORD HALL USAF MED CTR,DEPT PULM & SLEEP MED,LACKLAND AFB,TX 78236. RP Will, MJ (reprint author), BROOKE ARMY MED CTR,DEPT ORAL & MAXILLOFACIAL SURG,FT SAM HOUSTON,TX 78234, USA. NR 11 TC 32 Z9 32 U1 0 U2 2 PU AMER SLEEP DISORDERS ASSOC PI ROCHESTER PA 1610 14TH STREET NW SUITE 300, ROCHESTER, MN 55806 SN 0161-8105 J9 SLEEP JI Sleep PD DEC PY 1995 VL 18 IS 10 BP 873 EP 875 PG 3 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA TQ236 UT WOS:A1995TQ23600008 PM 8746394 ER PT J AU SHEKELLE, P MARKOVICH, M LOUIE, R AF SHEKELLE, P MARKOVICH, M LOUIE, R TI UNTITLED - RESPONSE SO SPINE LA English DT Letter C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP SHEKELLE, P (reprint author), RAND CORP,SANTA MONICA,CA 90406, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0362-2436 J9 SPINE JI SPINE PD DEC 1 PY 1995 VL 20 IS 23 BP 2596 EP 2598 PG 3 WC Clinical Neurology; Orthopedics SC Neurosciences & Neurology; Orthopedics GA TJ493 UT WOS:A1995TJ49300025 ER PT J AU Gordon, GH Baker, L Levinson, W AF Gordon, GH Baker, L Levinson, W TI Physician-patient communication in managed care SO WESTERN JOURNAL OF MEDICINE LA English DT Article ID SATISFACTION; SKILLS; PROGRAMS; BEHAVIOR AB The quality of physician-patient communication affects important health care outcomes, Managed care presents a number of challenges to physician-patient communication, including shorter visits, decreased continuity, and lower levels of trust. Good communication skills can help physicians create and maintain healthy relationships with patients in the face of these challenges, We describe 5 communication dilemmas that are common in managed care and review possible solutions suggested by recent literature on physician-patient communication, We also describe ways that managed care plans can promote more effective communication between physicians and patients. C1 OREGON HLTH SCI UNIV,SCH MED,LEGACY GOOD SAMARITAN HOSP,PORTLAND,OR 97201. NW CTR PHYSICIAN PATIENT COMMUN,PORTLAND,OR. RP Gordon, GH (reprint author), PORTLAND VET AFFAIRS MED CTR,MED SERV 111P,3710 SW US VET HOSP RD,PORTLAND,OR 97201, USA. NR 44 TC 27 Z9 29 U1 2 U2 2 PU CALIFORNIA PHYSICIAN MAGAZINE PI SAN FRANCISCO PA C/O DONNA TAYLOR, EDITOR, PO BOX 7690, SAN FRANCISCO, CA 94102-7690 SN 0093-0415 J9 WESTERN J MED JI West. J. Med. PD DEC PY 1995 VL 163 IS 6 BP 527 EP 531 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA TL443 UT WOS:A1995TL44300001 PM 8553634 ER PT J AU Rodriguez, TE Reddy, SV Roodman, GD AF Rodriguez, TE Reddy, SV Roodman, GD TI Cloning and characterization of a novel autocrine osteoclast stimulatory factor. SO BLOOD LA English DT Meeting Abstract C1 VET ADM MED CTR, SAN ANTONIO, TX USA. UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 EI 1528-0020 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 119 EP 119 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000119 ER PT J AU Alsina, M Devlin, R Reddy, SV Mundy, GR Boodman, GD AF Alsina, M Devlin, R Reddy, SV Mundy, GR Boodman, GD TI Cloning of a novel osteoclast stimulatory factor (OSF) expressed by ARH-77 cells and bone marrow cells from myeloma patients in vivo. SO BLOOD LA English DT Meeting Abstract C1 VET ADM MED CTR, SAN ANTONIO, TX USA. UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA SN 0006-4971 EI 1528-0020 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 213 EP 213 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000214 ER PT J AU Hirayama, F Ogawa, M AF Hirayama, F Ogawa, M TI Negative regulation of early T-lymphopoiesis by interleukin-3 and interleukin-1 alpha SO BLOOD LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. RALPH H JOHNSON VET AFFAIRS MED CTR,CHARLESTON,SC. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 630 EP 630 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000630 ER PT J AU Alcantara, O Reddy, SV Roodman, GD Boldt, DH AF Alcantara, O Reddy, SV Roodman, GD Boldt, DH TI A hemin responsive protein (HRP) involved in transcriptional regulation of tartrate resistant acid phosphatase (TRAP) gene expression is a novel ferritin gene product. SO BLOOD LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 667 EP 667 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000668 ER PT J AU Christodoulides, E Scott, EE Durante, W Olson, JS Schafer, AI AF Christodoulides, E Scott, EE Durante, W Olson, JS Schafer, AI TI A new method for the direct measurement of vascular smooth muscle cell derived carbon monoxide. SO BLOOD LA English DT Meeting Abstract C1 BAYLOR COLL MED,HOUSTON VET ADM MED CTR,HOUSTON,TX 77030. RICE UNIV,HOUSTON,TX 77251. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 783 EP 783 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000784 ER PT J AU Wagner, CT Durante, W Christodoulides, N Hellums, JD Schafer, AI AF Wagner, CT Durante, W Christodoulides, N Hellums, JD Schafer, AI TI Shear stress induces heme oxygenase and carbon monoxide production by vascular smooth muscle cells. SO BLOOD LA English DT Meeting Abstract C1 BAYLOR COLL MED,HOUSTON VET ADM MED CTR,HOUSTON,TX. RICE UNIV,HOUSTON,TX 77251. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 784 EP 784 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91000785 ER PT J AU Ku, H Kaushansky, K Ogawa, M AF Ku, H Kaushansky, K Ogawa, M TI Thrombopoietin, the ligand for the Mpl receptor, synergises with steel factor and other early-acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice. SO BLOOD LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. WASHINGTON UNIV,DEPT MED,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 1010 EP 1010 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91001012 ER PT J AU Friedberg, RC Wang, S AF Friedberg, RC Wang, S TI The impact upon platelet utilization of guidelines coupled with an aggressive transfusion medicine clinical consultation service SO BLOOD LA English DT Meeting Abstract C1 UNIV ALABAMA,BIRMINGHAM VAMC,DEPT PATHOL,PATHOL & LAB MED SERV,BIRMINGHAM,AL. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3411 EP 3411 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003412 ER PT J AU Friedberg, RC Bell, WC Sinor, LT AF Friedberg, RC Bell, WC Sinor, LT TI Prevalence of platelet-directed antibodies in surgical patients: Implications for platelet transfusion protocols. SO BLOOD LA English DT Meeting Abstract C1 UNIV ALABAMA,BIRMINGHAM VAMC,DEPT PATHOL,PATHOL & LAB MED SERV,BIRMINGHAM,AL. IMMUCOR INC,NORCROSS,GA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3412 EP 3412 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003413 ER PT J AU Friedberg, RC Huang, ST AF Friedberg, RC Huang, ST TI Provision of blood products to a patient with documented anaphylaxis to corn-derived compounds including ACD and CPD. SO BLOOD LA English DT Meeting Abstract C1 UNIV ALABAMA,BIRMINGHAM VAMC,DEPT PATHOL,PATHOL & LAB MED SERV,BIRMINGHAM,AL. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3413 EP 3413 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003414 ER PT J AU Messmore, HL Farid, S Fabbrini, N AF Messmore, HL Farid, S Fabbrini, N TI The anticoagulant effect of heparin is altered by a pentasaccharide. SO BLOOD LA English DT Meeting Abstract C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,HINES,IL 60141. LOYOLA UNIV,STRITCH SCH MED,MAYWOOD,IL 60153. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3514 EP 3514 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003515 ER PT J AU Messmore, HL Farid, S Fabbrini, N AF Messmore, HL Farid, S Fabbrini, N TI Effect of factor VIII levels on the calcium thrombin time (CATT) of heparinized normal plasma. SO BLOOD LA English DT Meeting Abstract C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,HINES,IL 60141. LOYOLA UNIV,STRITCH SCH MED,MAYWOOD,IL 60153. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3515 EP 3515 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003516 ER PT J AU Tsai, T Goodman, S Schiller, G Adkins, D Callander, N Wolff, S Freytes, C AF Tsai, T Goodman, S Schiller, G Adkins, D Callander, N Wolff, S Freytes, C TI Allogeneic bone marrow transplantation (Allo BMT) for relapse after autologous bone marrow transplantation (Auto BMT) in lymphomas and acute leukemia SO BLOOD LA English DT Meeting Abstract C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. NASHVILLE VA MED CTR,NASHVILLE,TN. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. WASHINGTON UNIV,SCH MED,ST LOUIS,MO 63110. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3864 EP 3864 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003864 ER PT J AU Tsai, T Salzman, D Clark, G Castro, J Callander, N Boldt, D Roodman, GD SheridanLeos, N Wiesner, A Adkins, D Harris, K Rodriguez, T Gokmen, E LeMaistre, CF Freytes, CO AF Tsai, T Salzman, D Clark, G Castro, J Callander, N Boldt, D Roodman, GD SheridanLeos, N Wiesner, A Adkins, D Harris, K Rodriguez, T Gokmen, E LeMaistre, CF Freytes, CO TI Augmented cyclophosphamide (C), carmustine (B) and etoposide (V) +/- dacarbazine (D) with autologous bone marrow transplantation (bmt) for relapsed or refractory Hodgkin's disease: Lower peritransplant mortality and analysis of prognostic factors. SO BLOOD LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,AUDIE L MURPHY MEM VET HOSP,SAN ANTONIO,TX 78284. S TEXAS CANC INST,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3865 EP 3865 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003865 ER PT J AU Jiang, CY Anderson, J Reedy, S Roodman, GD AF Jiang, CY Anderson, J Reedy, S Roodman, GD TI Construction of oncogene-containing retroviral expression vectors and their transforming capacity. SO BLOOD LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED HEMATOL,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD NOV 15 PY 1995 VL 86 IS 10 SU 1 BP 3987 EP 3987 PG 1 WC Hematology SC Hematology GA TH910 UT WOS:A1995TH91003987 ER PT J AU FEIGIN, AM ARONOV, EV BRYANT, BP TEETER, JH BRAND, JG AF FEIGIN, AM ARONOV, EV BRYANT, BP TEETER, JH BRAND, JG TI CAPSAICIN AND ITS ANALOGS INDUCE ION CHANNELS IN PLANAR LIPID BILAYERS SO NEUROREPORT LA English DT Article DE CAPSAICIN; ION CHANNELS; LIPID BILAYER ID ANALGESIC AGENTS; AGONIST ACTIVITY; REGION AB THE irritating, pungent compound, capsaicin (10-20 mu M), induces the formation of non-selective ion channels with a wide variety of conductances in protein-free lipid bilayers formed from a mixture of zwitterionic phospholipids. The channel-forming activity of capsaicin and four of its analogs followed the sequence: resiniferatoxin > capsaicin = pelargonic acid vanillylamide > methylcapsaicin >> veratrylamine. The ability to form channels correlated with the biological activity of these compounds observed in other studies that measured Ca-45 uptake into rat dorsal root ganglion cells. The correlation obtained suggests that an interaction with the lipid bilayer may be an important component of the biological activity of capsaicin. C1 UNIV PENN,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP FEIGIN, AM (reprint author), MONELL CHEM SENSES CTR,3500 MARKET ST,PHILADELPHIA,PA 19104, USA. NR 14 TC 27 Z9 27 U1 1 U2 5 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0959-4965 J9 NEUROREPORT JI Neuroreport PD NOV 13 PY 1995 VL 6 IS 16 BP 2134 EP 2136 DI 10.1097/00001756-199511000-00009 PG 3 WC Neurosciences SC Neurosciences & Neurology GA TH461 UT WOS:A1995TH46100008 PM 8595187 ER PT J AU KUDLICKI, W CHIRGWIN, J KRAMER, G HARDESTY, B AF KUDLICKI, W CHIRGWIN, J KRAMER, G HARDESTY, B TI FOLDING OF AN ENZYME INTO AN ACTIVE CONFORMATION WHILE BOUND AS PEPTIDYL-TRANSFER-RNA TO THE RIBOSOME SO BIOCHEMISTRY LA English DT Article ID TRANSLATION; PROTEINS AB Rhodanese bound to bacterial ribosomes as peptidyl-tRNA can be folded into an enzymatically active conformation by generating C-terminal extensions of the wild-type enzyme. Rhodanese was synthesized by coupled transcription/translation in a cell-free Escherichia coli system from plasmids containing the coding sequences for the wild-type enzyme or its C-terminally extended mutants. Two proteins with extensions of 23 amino acids or longer were enzymatically active while bound to the ribosomes whereas wild-type protein and a 13-amino acid extension were not. All forms of the enzyme were active after termination and release of the full-length protein from the ribosomes. All five of the bacterial chaperones were required to substantially increase the specific enzymatic activity of the extended rhodanese while the nascent protein was bound to ribosomes. The results provide direct support for the hypothesis that proteins acquire tertiary structure as they are formed in ribosomes. C1 UNIV TEXAS,DEPT CHEM & BIOCHEM,AUSTIN,TX 78712. AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. NR 18 TC 45 Z9 46 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA PO BOX 57136, WASHINGTON, DC 20037-0136 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD NOV 7 PY 1995 VL 34 IS 44 BP 14284 EP 14287 DI 10.1021/bi00044a003 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TD845 UT WOS:A1995TD84500003 PM 7578030 ER PT J AU CHOI, SJ OH, DH SONG, CS ROY, AK CHATTERJEE, B AF CHOI, SJ OH, DH SONG, CS ROY, AK CHATTERJEE, B TI MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE RAT-LIVER CARNITINE OCTANOYLTRANSFERASE CDNA, ITS NATURAL GENE AND THE GENE PROMOTER SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION LA English DT Article DE CARNITINE OCTANOYLTRANSFERASE; CDNA; PROMOTER; PEROXISOME; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR ID ACYL-COA OXIDASE; PEROXISOMAL ENOYL-COA; BIFUNCTIONAL ENZYME; TRANSCRIPTIONAL REGULATION; NUCLEOTIDE-SEQUENCE; BETA-OXIDATION; MESSENGER-RNA; MALONYL-COA; DEHYDROGENASE; DNA AB The full-length cDNA and the natural gene for rat peroxisomal carnitine octanoyltransferase (COT) have been isolated and sequenced. The 2681 bp long cDNA contains an open reading frame for 613 amino acids, resulting in a protein with a deduced molecular weight of 70 301, and a C-terminal peroxisomal targeting sequence (Ala-His-Leu). The isolated COT cDNA has 51 bp of the 5' untranslated region (UTR), 791 bp of 3' UTR, two putative polyadenylation sites, and a poly(A(19-23)) tail. Screening of a rat genomic DNA library in the lambda phage with the COT cDNA probe resulted in the isolation of seven overlapping clones, together containing the complete COT gene with seventeen exons. All of the exon-intron boundary sequences conform to the GT-AG rule. The COT gene appears to spread over 40 to 60 kbp region of the rat genome. The transcription initiation site of the COT gene was determined through primer extension, and the promoter sequence up to the position -1140 was established. The promoter lacks the canonical TATA box and a promoter-reporter construct containing the sequence encompassing - 1140 to + 84 base positions and the firefly luciferase reporter cDNA yielded about 100-fold increase in promoter activity in transfected hepatoma cells. Some of the consensus sequences for putative cis elements present in the promoter sequence are: the two CCAAT motifs for CTF/NF1/CBP binding (at - 284 and - 93), two GC boxes for Sp1 binding (at - 160 and - 68), two AP2 sites (at - 359 and - 25), a half site (TGACCT) for the peroxisome proliferator activated receptor (PPAR) binding at - 737 within a partial palindromic sequence region. Potential regulatory elements, such as several palindromes and repeat motifs for five different sequence segments, are also identified. C1 UNIV TEXAS,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,SAN ANTONIO,TX 78284. YONSEI UNIV,BIOPROD RES CTR,SEOUL 120749,SOUTH KOREA. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NIDDK NIH HHS [R37 DK-14744] NR 47 TC 24 Z9 25 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4781 J9 BBA-GENE STRUCT EXPR JI Biochim. Biophys. Acta-Gene Struct. Expression PD NOV 7 PY 1995 VL 1264 IS 2 BP 215 EP 222 DI 10.1016/0167-4781(95)00146-8 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA TE679 UT WOS:A1995TE67900012 PM 7495866 ER PT J AU DELEO, FR NAUSEEF, WM JESAITIS, AJ BURRITT, JB CLARK, RA QUINN, MT AF DELEO, FR NAUSEEF, WM JESAITIS, AJ BURRITT, JB CLARK, RA QUINN, MT TI A DOMAIN OF P47(PHOX) THAT INTERACTS WITH HUMAN NEUTROPHIL FLAVOCYTOCHROME B(558) SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CHRONIC GRANULOMATOUS-DISEASE; RESPIRATORY BURST OXIDASE; CELL-FREE SYSTEM; NADPH OXIDASE; MICROBICIDAL OXIDASE; PLASMA-MEMBRANE; SUPEROXIDE PRODUCTION; CYTOSOLIC COMPONENTS; CYTOCHROME-B; BINDING SITE AB The NADPH-dependent oxidase of human neutrophils is a multicomponent system including cytosolic and membrane proteins. Activation requires translocation of cytosolic proteins p47(phox), p67(phox) ,and Rac2 to the plasma membrane and association with the membrane flavocytochrome b to assemble a functioning oxidase. We report the location of a region in p47(phox) that mediates its interaction with flavocytochrome b. From a random peptide phage display Library, we used biopanning with purified flavocytochrome b to select phage peptides that mimicked potential p47(phox) binding residues. Using this approach, we identified a region of p47(phox) from residue 323 to 342 as a site of interaction with flavocytochrome b. Synthetic peptides (315)SRKRLSQD-AYRRNS(328), (323)AYRRNSVRFL(332) and (334)QRRRQARPG-PQSPG(347) inhibited superoxide (O-2(radical anion)) production in the broken cell system with IC, of 18, 57, and 15 mu M, respectively. (323)AYRRNSVRFL(332) and its derivative peptides inhibited phosphorylation of p47(phox). However, the functional importance of this peptide was independent of its effects on phosphorylation, since (323)AYRRNA-VRFL(332) inhibited O-2(radical anion) production, but had no effect on phosphorylation. None of the peptides blocked O-2(radical anion) production when added after enzyme activation, suggesting that they inhibited the assembly, rather than the activity, of the oxidase. Furthermore these peptides inhibited membrane association of p47(phox), the broken cell translocation assay and O-2(radical anion) production by electropermeabilized neutrophils, thereby supporting the interpretation that this region of p47(phox) interacts with flavocytochrome b. C1 MONTANA STATE UNIV,DEPT VET MOLEC BIOL,BOZEMAN,MT 59717. MONTANA STATE UNIV,DEPT MICROBIOL,BOZEMAN,MT 59717. UNIV IOWA,IOWA CITY,IA 52242. DEPT VET AFFAIRS MED CTR,DEPT MED,IOWA CITY,IA 52242. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. OI DeLeo, Frank/0000-0003-3150-2516 FU BLRD VA [I01 BX000513]; NIAID NIH HHS [AI28412, R37 AI020866, R01 AI020866, AI20866]; NIAMS NIH HHS [AR40929] NR 49 TC 65 Z9 65 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD NOV 3 PY 1995 VL 270 IS 44 BP 26246 EP 26251 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TC978 UT WOS:A1995TC97800038 PM 7592831 ER PT J AU GUZE, PA AF GUZE, PA TI CULTIVATING CURRICULAR REFORM SO ACADEMIC MEDICINE LA English DT Article; Proceedings Paper CT AAMCs Consensus Conference on the Education of Medical Students about Family Violence and Abuse CY MAR 28-29, 1995 CL WASHINGTON, DC SP Assoc Amer Med Coll ID MEDICAL-EDUCATION AB Since the 1960s there has been pressure to reform medical education in a more humanistic direction. One reason this has been difficult is that most medical schools have been forced to maintain themselves on resources allocated to support research and the technology of specialized tertiary care. Nevertheless, many people,believe that medical-education can still change because of changes taking place outside the sciences, such as a redefinition-of the meaning of health and the need to provide better health care to the U.S. population at a lower cost. Taking this-optimistic view will help strengthen reformers' resolve for curricular change and the incorporation of important areas such as family violence into medical students' education. There are numerous barriers to curricular change. Yet there are useful principles that can guide reform efforts, such as having an explicit rationale for the desired change, focusing on educational goals rather than on resources for their implementation, recruiting support from the departmental and school leadership, anticipating negative reactions, and recognizing the need for negotiation. There are also principles to foster successful implementation, the most important of which is to have everyone involved agree an the goals of the new program and participate in the process. The way to increase an emphasis on family violence issues is to fmd areas in the curriculum where these issues can be integrated with current teaching. Finally, a medical school curriculum on family violence does not need to be all-inclusive, but instead should prepare a good foundation so that students tan expand their knowledge and skills during residency training and medical practice. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,AFFILIATED PROGRAMS,LOS ANGELES,CA. RP GUZE, PA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 3 TC 14 Z9 14 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD NOV PY 1995 VL 70 IS 11 BP 971 EP 973 DI 10.1097/00001888-199511000-00013 PG 3 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA TF412 UT WOS:A1995TF41200018 PM 7575951 ER PT J AU FRAZAO, J COBURN, JW AF FRAZAO, J COBURN, JW TI SYMPTOMATIC HYPERCALCEMIA IN A DIABETIC PATIENT UNDERGOING CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS - VALUE OF BONE-BIOPSY IN THE DIAGNOSIS AND MANAGEMENT SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE HYPERCALCEMIA; DIABETES MELLITUS; CONTINUOUS AMBULATORY PERITONEAL DIALYSIS; BONE BIOPSY; OSTEITIS FIBROSA ID PARATHYROID-GLAND RESPONSIVENESS; RENAL OSTEODYSTROPHY; HEMODIALYSIS-PATIENTS; HYPOCALCEMIA; OSTEOMALACIA; FAILURE; UREMIA AB An elderly man with diabetes mellitus and end-stage renal disease managed with continuous ambulatory peritoneal dialysis (CAPD) was hospitalized with peripheral vascular insufficiency; he developed hypercalcemia and became mentally obtunded, Lowering dialysate Ca from 3.5 mEq/L to 2.5 mEq/L, stopping calcium acetate, and ultimately hemodialysis with calcium-free dialysate did not lead to reversal of the hypercalcemia or improvement of his symptoms, The intact parathyroid hormone PTH level was 187 pg/mL, a value rarely associated with significant osteitis fibrosa, A search for other causes of hypercalcemia was unrevealing, and a iliac crest bone biopsy was done, The latter showed osteitis fibrosa, and the patient underwent parathyroidectomy, The hypercalcemia reversed quickly, and his mental symptoms slowly improved, The discussion reviews the probable causes of hypercalcemia in diabetic patient undergoing CAPD with 3.5 mEq/L dialysate calcium and using calcium-containing phosphate binders, with hyperparathyroidism certainly not the usual cause, The reason for the occurrence of significant hyperparathyroidism in the face of only modest elevation of PTH is considered, The value of bone biopsy in resolution of this problem is apparent. (C) 1995 by the National Kidney Foundation, Inc. C1 W LOS ANGELES VET AFFAIRS MED CTR,WADSWORTH DIV,MED SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,WADSWORTH DIV,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. RI Frazao, Joao/J-9811-2013 OI Frazao, Joao/0000-0002-8081-5474 NR 27 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0272-6386 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD NOV PY 1995 VL 26 IS 5 BP 831 EP 835 DI 10.1016/0272-6386(95)90452-2 PG 5 WC Urology & Nephrology SC Urology & Nephrology GA TC988 UT WOS:A1995TC98800021 PM 7485141 ER PT J AU BARNES, JL TORRES, ES MITCHELL, RJ PETERS, JH AF BARNES, JL TORRES, ES MITCHELL, RJ PETERS, JH TI EXPRESSION OF ALTERNATIVELY SPLICED FIBRONECTIN VARIANTS DURING REMODELING IN PROLIFERATIVE GLOMERULONEPHRITIS SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID GROWTH-FACTOR-BETA; MESANGIAL CELL-MIGRATION; HABU SNAKE-VENOM; EXTRACELLULAR-MATRIX; MESSENGER-RNA; GENE-EXPRESSION; COLLAGEN; REGION; RAT; LOCALIZATION AB Fibronectin (Fn) plays an important role in tissue remodeling during embryogenesis, wound repair, and vascular disease, and is thought to regulate cellular processes such as cell adhesion, migration, proliferation, and differentiation through specialized domains within the molecule. In addition, Fn can be alternatively spliced at three regions extra domains EIIIA, EIIIB,and a variable segment V, potentially giving rise to functionally distinct variants of the molecule. We have previously shown a sequential expression of cellular Tn first by platelets, followed by macrophages, then mesangial cells in habu snake venom-induced proliferative glomerulonephritis (Am J Pathol 145: 585-597, 1994) These studies examined the cellular sources and glomerular localization of Fn in general but did not distinguish between the various alternatively spliced isoforms. In this study, we examine by in situ hybridization and immunohistochemistry the temporal expression and cellular sources of EIIIA, EIIIB, and V in a model of proliferative glomerulonephritis that has cell migration, proliferation, and extracellular matrix synthesis as features of tissue remodeling. Macrophages were the first cells to express Fn mRNA showing an EIIIA(+), EIIIB(-), and V95(+) pattern beginning at 8 hours after habu snake venom injection, Migrating mesangial cells at the margins of early lesions (8 and 24 hours) dirt not overexpress mRNA encoding these En variants, but immunofluorescence microscopy revealed V95 and EIIIA protein at the margins of lesions. EIIIB was absent in lesions at this time. At 48 hours and peaking at 72 hours after habu snake venom injection, mesangial cells in central aspects of glomerular lesions expressed abundant mRNA and protein for V95 and EIIIA. EIIIB mRNA and protein was slight in the mesangium at these times. Parietal epithelial cells, particularly adjacent to glomerular lesions, also expressed abundant mRNA and protein for all three variants throughout the course of the disease, beginning at 24 hours after habu snake venom injection. Expression of mRNA and protein for all three isoforms declined by 2 weeks after habu snake venom injection. These studies show that migrating mesangial cells do not require their own synthesis of Fn and suggest that they might rely on exogenous sources of Fn, particularly V95(+) and EIIIA(+) forms. Commencement of enhanced expression of EIIIA and EIIIB mRNA and protein by resident glomerular cells coincided with the temporal course of cell proliferation, acquisition of alpha-smooth muscle cell actin phenotype, and matrix synthesis, suggesting that Fn isoforms have specific functions during the course of glomerular remodeling. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. CEDARS SINAI MED CTR,DIV PULM MED,LOS ANGELES,CA 90048. RP BARNES, JL (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV NEPHROL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIDDK NIH HHS [DK 38758] NR 47 TC 48 Z9 48 U1 0 U2 0 PU AMER SOC INVESTIGATIVE PATHOLOGY, INC PI BALTIMORE PA 428 EAST PRESTON ST, BALTIMORE, MD 21202-3993 SN 0002-9440 J9 AM J PATHOL JI Am. J. Pathol. PD NOV PY 1995 VL 147 IS 5 BP 1361 EP 1371 PG 11 WC Pathology SC Pathology GA TD741 UT WOS:A1995TD74100020 PM 7485399 ER PT J AU CROWLEY, ST RAY, CJ NAWAZ, DF MAJACK, RA HORWITZ, LD AF CROWLEY, ST RAY, CJ NAWAZ, DF MAJACK, RA HORWITZ, LD TI MULTIPLE GROWTH-FACTORS ARE RELEASED FROM MECHANICALLY INJURED VASCULAR SMOOTH-MUSCLE CELLS SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE ANGIOPLASTY; RESTENOSIS; PROLIFERATION ID ENDOTHELIAL-CELLS; GENE-EXPRESSION; ARTERIAL INJURY; WOUND REPAIR; ANTIBODY; COLLAGENASE; ANGIOPLASTY; RESTENOSIS; INDUCTION; RECEPTOR AB Local release of mitogenic and chemotactic signals during angioplasty-induced vascular injury may initiate restenosis. We investigated whether mechanical injury to vascular smooth muscle cells (VSMC) results in the release of biologically active peptide growth factors. Monolayers of bovine SMC cultures were mechanically injured by cell scraping. Conditioned medium (CM) from control and injured SMC cultures was collected, and the mitogenic activity was measured by [H-3]thymidine incorporation in recipient SMC cultures. Mitogenic activity from injured CM was detected within 15 min after injury. When the CM from injured cells was removed 15 min after injury and replaced with serum-free media, there was no detectabe mitogenic activity in the replacement CM assessed 1-6 days postinjury. Suramin, a nonspecific peptide growth factor antagonist, significantly inhibited the mitogenic activity of injured CM. Basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF A chain), and epidermal growth factor (EGF) were detected in CM from injured cells by immunoblot analysis. The mitogenic activity of injured CM was significantly inhibited with neutralizing antibodies to bFGF (34%), PDGF-AA (32%), PDGF-BB (25%), and EGF (25%). A neutralizing antibody to tranforming growth factor (TGF)-beta had no effect. In conclusion, bFGF, PDGF, and EGF are immediately released from mechanically injured VSMC. VSMC Likely contain preformed, biologically active growth factors that are efficiently released from the cell cytoplasm following mechanical injury. Conditioned medium from injured VSMC is highly mitogenic, and this activity is probably due to multiple growth factors interacting synergistically. C1 DENVER VET AFFAIRS MED CTR, DIV CARDIOL, DENVER, CO 80220 USA. UNIV COLORADO, SCH MED, DEPT PEDIAT, DENVER, CO 80262 USA. UNIV COLORADO, SCH MED, DIV CARDIOL, DENVER, CO 80262 USA. FU NHLBI NIH HHS [HL-48177] NR 33 TC 64 Z9 66 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD NOV PY 1995 VL 269 IS 5 BP H1641 EP H1647 PG 7 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA TF258 UT WOS:A1995TF25800019 PM 7503260 ER PT J AU BEUTLER, AM SCHUMACHER, HR WHITTUMHUDSON, JA SALAMEH, WA HUDSON, AP AF BEUTLER, AM SCHUMACHER, HR WHITTUMHUDSON, JA SALAMEH, WA HUDSON, AP TI IN-SITU HYBRIDIZATION FOR DETECTION OF INAPPARENT INFECTION WITH CHLAMYDIA-TRACHOMATIS IN SYNOVIAL TISSUE OF A PATIENT WITH REITERS-SYNDROME SO AMERICAN JOURNAL OF THE MEDICAL SCIENCES LA English DT Note DE CHLAMYDIAL INFECTION; REITERS SYNDROME; IN SITU HYBRIDIZATION; SYNOVIUM ID INSITU DNA HYBRIDIZATION; REACTIVE ARTHRITIS; CULTURE; DISEASE; WOMEN; INFERTILITY; CRITERIA; ANTIBODY; SMEARS; ACID AB The authors have shown that protein antigens, RNA, and DNA from Chlamydia trachomatis are present in synovial tissues of patients with Reiter's syndrome (RS). However, those studies gave no insight into the host cell type involved or the precise tissue location of the bacteria, To address such issues, the authors developed an in situ hybridization system to detect chlamydia, and they used that system to examine synovial biopsies from a patient with RS and a patient without RS. The in situ system uses a previously described digoxigenin-labeled DNA probe that hybridizes with chlamydial 16S rRNA sequences in paraformaldehyde-fixed samples. Control studies with chlamydia-infected and uninfected HeLa cells confirmed that the in situ system is as sensitive as is direct fluorescence cytology for detection of the organism. Morphology of host and chlamydia cells is preserved after hybridization. Studies using synovial tissue from an osteoarthritis patient produced no in hybridization signal, but similar hybridization to tissue from a culture-/direct fluorescence cytology- negative RS patient had a strong intracellar signal for chlamydia within a subsynovial cell layer. These in situ hybridization results confirm the extensive presence of chlamydia in synovia and extend the authors' earlier observation that chlamydial RNA is present in the synovia of patients with RS, The data also confirm their electron microscopy studies, indicating that chlamydia are intracellular in synovial tissue, and they further show that infected host cells are located beneath the synovial lining. C1 DEPT VET AFFAIRS MED CTR,MED RES SERV,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DEPT MED,DIV RHEUMATOL,PHILADELPHIA,PA. DEPT VET AFFAIRS MED CTR,CTR ARTHRIT & IMMUNOL,PHILADELPHIA,PA 19104. TEMPLE UNIV,SCH MED,DEPT OBSTET & GYNECOL,DIV REPROD ENDOCRINOL,PHILADELPHIA,PA. MED COLL PENN,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19129. JOHNS HOPKINS UNIV,SCH MED,WILMER INST,BALTIMORE,MD 21205. FU NEI NIH HHS [EY-O3324]; NIAMS NIH HHS [AR-42541] NR 38 TC 40 Z9 40 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0002-9629 J9 AM J MED SCI JI Am. J. Med. Sci. PD NOV PY 1995 VL 310 IS 5 BP 206 EP 213 DI 10.1097/00000441-199511000-00006 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA TD448 UT WOS:A1995TD44800006 PM 7485225 ER PT J AU CARROLL, JC NELSON, VS HURVITZ, EA PRIEBE, M AF CARROLL, JC NELSON, VS HURVITZ, EA PRIEBE, M TI HOME MECHANICAL VENTILATION IN MITOCHONDRIAL ENCEPHALOMYOPATHY SYNDROME SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Note ID STROKE-LIKE EPISODES; LACTIC-ACIDOSIS; MYOPATHY; ENCEPHALOPATHY; DNA AB Long-term home mechanical ventilation of children has only recently become more practically feasible and ethically acceptable by the medical community. It has been particularly controversial in cases of degenerative myopathies in which quality of life has been questioned. There are no reports in the literature of long-term home mechanical ventilation of a child with mitochondrial encephalomyopathy (MELAS) syndrome despite the many descriptions of possible etiologies of the concomitant respiratory failure. The patient reported here has used home mechanical ventilation for 6 years with few medical complications, no hospitalizations in the past 3 years, and increased function in activities of daily living. Despite the ill-defined nature of the disease and uncertain prognosis, we believe that long-term home mechanical ventilation of children with early onset MELAS syndrome is a viable option for both patients and their families and results in overall improvement in quality of life for the patient. (C) 1995 by the American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation. C1 UNIV MICHIGAN,MED CTR,DEPT PHYS MED & REHABIL,ANN ARBOR,MI 48109. UNIV MICHIGAN,MED CTR,DEPT PEDIAT & COMMUNICABLE DIS,ANN ARBOR,MI 48109. ANNA ARBOR MED CTR,DEPT VET AFFAIRS,ANN ARBOR,MI. DEPT VET AFFAIRS MED CTR,BAYLOR COLL MED,DEPT PHYS MED & REHABIL,HOUSTON,TX. NR 20 TC 1 Z9 1 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD NOV PY 1995 VL 76 IS 11 BP 1014 EP 1016 DI 10.1016/S0003-9993(95)81040-4 PG 3 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA TB712 UT WOS:A1995TB71200008 PM 7487448 ER PT J AU PRABHU, SD FREEMAN, GL AF PRABHU, SD FREEMAN, GL TI POSTEXTRASYSTOLIC MECHANICAL RESTITUTION IN CLOSED-CHEST DOGS - EFFECT OF HEART-FAILURE SO CIRCULATION LA English DT Article DE TACHYCARDIA; HEART FAILURE; MECHANICS; CONTRACTILITY; SYSTOLE ID END-SYSTOLIC PRESSURE; CANINE VENTRICULAR MYOCARDIUM; MAMMALIAN MYOCARDIUM; VOLUME RELATIONSHIP; CONTRACTILE STATE; PAPILLARY-MUSCLE; CONSCIOUS DOGS; INTERVAL; FORCE; STIMULATION AB Background Postextrasystolic mechanical restitution (MR(PES)) is thought to be an expression of intracellular Ca2+ handling by cardiac sarcoplasmic reticulum (SR). Since congestive heart failure is characterized by abnormal intracellular Ca2+ homeostasis, we sought to delineate MR,, behavior before and after the production of heart failure to obtain insights into the relation between altered mechanical performance and Ca2+ handling. Methods and Results Ten dogs instrumented with left ventricular (LV) micromanometers and piezoeleetric dimension crystals were studied under control conditions; 6 dogs also were studied after tachycardia heart failure (THF) produced by rapid LV pacing for 4 weeks. After priming at a basic cycle length of 375 ms, test pulses were delivered at fixed extrasystolic intervals (ESIs; 300, 375, or 450 ms) and graded postextrasystolic intervals (PESIs). Postextrasystolic mechanical response was assessed using single-beat elastance. MR,, curves were constructed by expressing normalized mechanical response as a function of the PESI. Control MR(PES) was a monoexponential function whose time constant (TC) and PESI-(axis) intercept (PESI(0)) increased significantly (P<.01) with increases in the antecedent ESI. THF significantly slowed MR(PES) kinetics at each antecedent ESI (P<.025), increased normalized maximal contractile response (CR(max) P<.01), and shortened PESI(0) (P<.025). Increases in the TC and CR(max) were most pronounced with the smallest antecedent ESI (percent control postextrasystolic TC 363.7 +/- 60.5%, ESI of 300 ms versus 139.0 +/- 15.1%, ESI of 450 ms, P<.005; percent control CR(max), 128.6 +/- 4.9%, ESI of 300 ms versus 104.9 +/ -1.0%, ESI of 450 ms; P<.005). Conclusions MR(PES) is much less dynamic in THF: The failing heart operates at lower levels of contractile performance after higher stimulation frequencies and cannot increase its speed of contractile recovery to compensate for higher heart rate. Prolongation of M(PES) kinetics is consistent with depression of SR Ca2+ release mechanisms in THF and implicates this site in the loss of the capacity of the failing heart to maintain mechanical performance with tachycardia. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RI Prabhu, Sumanth/D-5223-2009 NR 42 TC 18 Z9 18 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD NOV 1 PY 1995 VL 92 IS 9 BP 2652 EP 2659 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TC796 UT WOS:A1995TC79600040 PM 7586369 ER PT J AU MORENO, F GROTA, P CRISP, C MAGNON, K MELCHER, GP JORGENSEN, JH PATTERSON, JE AF MORENO, F GROTA, P CRISP, C MAGNON, K MELCHER, GP JORGENSEN, JH PATTERSON, JE TI CLINICAL AND MOLECULAR EPIDEMIOLOGY OF VANCOMYCIN-RESISTANT ENTEROCOCCUS-FAECIUM DURING ITS EMERGENCE IN A CITY IN SOUTHERN TEXAS SO CLINICAL INFECTIOUS DISEASES LA English DT Article AB During a 19-month period from April 1993 to October 1994, 41 isolates of vancomycin-resistant Enterococcus faecium (VREF) were detected in seven different hospitals in a city in southern Texas. A case-control study to determine the risk factors for acquisition was done in the hospital in which the majority of isolates were detected. Pulsed-field gel electrophoresis (PFGE) of whole-cell DNA was used to determine strain identity. Thirty-five (85%) of the 41 VREF isolates were of the vanB phenotype. Of these, 32 (91%) of 35 were the same strain by PFGE typing. The same vanB strain was documented in five different hospitals in the city. In contrast, 4 (67%) of 6 of the vanA phenotype VREF isolates were distinct strains by PFGE typing. Significant risk factors for colonization or infection with VREF were prior exposure to antibiotics (P=.04), the previous use of third-generation cephalosporins (P=.03), and the previous use of parenteral vancomycin (P=.002). Infection-control and antibiotic-utilization measures were implemented to control cross-transmission and selection of VREF isolates. During the emergence of VREF in our city, clonal dissemination of a single strain of vanB VREF among six hospitals was documented. Limited cross-transmission of vanA phenotype VREF isolates occurred, but most vanA VREF isolates were distinct strains selected in individual hospital environments. C1 UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. BAPTIST MEM HOSP,SAN ANTONIO,TX. WILFORD HALL USAF MED CTR,SAN ANTONIO,TX 78236. NR 12 TC 100 Z9 100 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD NOV PY 1995 VL 21 IS 5 BP 1234 EP 1237 DI 10.1093/clinids/21.5.1234 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA TC172 UT WOS:A1995TC17200025 PM 8589148 ER PT J AU KANDULA, P SHIRAZI, P AF KANDULA, P SHIRAZI, P TI LOCALIZATION OF TC-99M SESTAMIBI AND TL-201 IN AN UNSUSPECTED CALCIFIED INTRATHORACIC MASS SO CLINICAL NUCLEAR MEDICINE LA English DT Article ID INCREASED ACCUMULATION; TUMOR; TECHNETIUM-99M-SESTAMIBI; LESIONS; MIBI AB Tc-99m MIBI and TI-201 uptake have been reported in benign and malignant lesions. However, concordant uptake of both radiotracers has been described infrequently. The authors report a patient with a case history of tuberculosis and breast cancer who underwent a TI-201 cardiac stress/planar study initially for complaints of angina pectoris, and 3 years later a follow-up Tc-99m MIBI cardiac study was completed for similar complaints. These studies incidentally revealed uptake of TI-201 and Tc-99m MIBI in a large left lung mass unchanged in size between the two studies. CT with intravenous contrast performed 2 months after the Tc-99m MIBI study revealed a 9 cm calcified soft-tissue mass in the left lung with adjacent pleural calcifications. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,DEPT NUCL MED,NUCL MED SERV 115,HINES,IL 60141. NR 13 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0363-9762 J9 CLIN NUCL MED JI Clin. Nucl. Med. PD NOV PY 1995 VL 20 IS 11 BP 1000 EP 1002 DI 10.1097/00003072-199511000-00014 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TE189 UT WOS:A1995TE18900014 PM 8565352 ER PT J AU WEINSTOCK, R LEONG, GB AF WEINSTOCK, R LEONG, GB TI ETHICAL ISSUES IN PSYCHIATRY SO CURRENT OPINION IN PSYCHIATRY LA English DT Article AB Ongoing ethical dilemmas in psychiatry continue in those areas in which legal and social interests compete with the profession's concern for patient welfare. This is especially highlighted by the psychiatric ethical problems raised by the recent renewed societal interest in euthanasia and physician-assisted suicide. C1 UNIV CALIF LOS ANGELES,PSYCHIAT SERV,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA. HARRY S TRUMAN MEM VET HOSP,PSYCHIAT SERV,COLUMBIA,MO 65201. UNIV MISSOURI,DEPT NEUROL & PSYCHIAT,COLUMBIA,MO. NR 21 TC 0 Z9 0 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0951-7367 J9 CURR OPIN PSYCHIATR JI Curr. Opin. Psychiatr. PD NOV PY 1995 VL 8 IS 6 BP 383 EP 385 DI 10.1097/00001504-199511000-00008 PG 3 WC Psychiatry SC Psychiatry GA TG506 UT WOS:A1995TG50600008 ER PT J AU MEYER, JS OBARA, K MURAMATSU, K MORTEL, KF SHIRAI, T AF MEYER, JS OBARA, K MURAMATSU, K MORTEL, KF SHIRAI, T TI COGNITIVE PERFORMANCE AFTER SMALL STROKES CORRELATES WITH ISCHEMIA, NOT ATROPHY OF THE BRAIN SO DEMENTIA LA English DT Article DE VASCULAR DEMENTIA; CEREBRAL ATROPHY; RECURRENT INFARCTION; CEREBRAL BLOOD FLOW ID MULTI-INFARCT DEMENTIA; CEREBRAL BLOOD-FLOW; RISK-FACTORS; MULTIINFARCT DEMENTIA; COMPUTED-TOMOGRAPHY; VASCULAR DEMENTIA; IMPAIRMENT; DEFICITS; LESIONS; COHORT AB Computerized tomographic measures of recurrent cerebral infarctions, atrophy and local perfusion were all prospectively correlated with cognitive testing during treatment of risk factors plus antiplatelet therapy among vascular dementia patients. Neurological and cognitive status were quantified among 22 demented patients with small strokes and compared with 22 age-matched normal volunteers. In vascular dementia, risk factor control plus antiplatelet therapy reduced cerebral infarctions, increased perfusion, and stabilized or improved cognitive test performance, despite age-related, progressive cerebral atrophy. C1 BAYLOR COLL MED,DEPT VET AFFAIRS MED CTR,CEREBROVASC RES LABS,HOUSTON,TX 77030. NR 51 TC 15 Z9 16 U1 1 U2 2 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1013-7424 J9 DEMENTIA JI Dementia PD NOV-DEC PY 1995 VL 6 IS 6 BP 312 EP 322 PG 11 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA TD390 UT WOS:A1995TD39000003 PM 8563784 ER PT J AU HANDFORTH, A MAI, T TREIMAN, DM AF HANDFORTH, A MAI, T TREIMAN, DM TI RISING DOSE STUDY OF SAFETY AND TOLERANCE OF FLUNARIZINE SO EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY LA English DT Article DE FLUNARIZINE; SEIZURES ID PLACEBO-CONTROLLED TRIAL; REFRACTORY CHILDHOOD EPILEPSY; DOUBLE-BLIND CROSSOVER; ADD-ON THERAPY AB In a recent NIH-sponsored parallel-group placebo-controlled blinded study of flunarizine for the treatment of partial-onset seizures, the flunarizine serum concentration was controlled to a constant level among patients in order to reduce response variability. Flunarizine was found to exhibit modest anti-epileptic efficacy. A potential criticism of this study is that the chosen controlled concentration was too low to determine optimal efficacy. As a participating center in this study we investigated the effect of higher doses of open-label flunarizine on seizure frequency in 16 patients with refractory partial seizures. Following the completion of the blinded placebo/flunarizine phase, all patients were initiated at the flunarizine dose calculated to result in a serum concentration of 60 ng . ml(-1). The dose was subsequently increased each 8-12 weeks to a maximum of 2.7 times the initial dose. On the initial maintenance flunarizine dose, seizure control was improved, with an average seizure reduction of 47% compared to pre-blinded-phase baseline. When higher doses were administered, adverse reactions were more common yet improved seizure control did not occur in most patients. These findings complement those of the concentration-controlled NIH study and suggest that appropriate flunarizine doses were utilized in that study. C1 W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. RP HANDFORTH, A (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEUROL SERV,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. FU NINDS NIH HHS [N01-NS-7-2328] NR 9 TC 6 Z9 7 U1 1 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0031-6970 J9 EUR J CLIN PHARMACOL JI Eur. J. Clin. Pharmacol. PD NOV PY 1995 VL 49 IS 1-2 BP 91 EP 94 PG 4 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TE475 UT WOS:A1995TE47500016 PM 8751028 ER PT J AU UBEL, PA LOEWENSTEIN, G AF UBEL, PA LOEWENSTEIN, G TI THE EFFICACY AND EQUITY OF RETRANSPLANTATION - AN EXPERIMENTAL SURVEY OF PUBLIC-ATTITUDES SO HEALTH POLICY LA English DT Article DE TRANSPLANTATION; EQUITY; PROGNOSIS; ETHICS; HEALTH POLICY ID HEALTH-CARE PRIORITIES; OREGON; TRANSPLANTATION; ORGANS AB Purpose: To measure the relative importance people place on prognosis and retransplantation status in allocating scarce transplantable livers. Methods: 138 subjects were asked to distribute scarce livers amongst transplant candidates with either a 70% chance or a 30% chance of surviving if transplanted, In one group of subjects, the prognostic difference was based on the presence or absence of a 'blood marker.' In the other group, the prognostic difference was based on whether candidates had been previously transplanted or not, with retransplant candidates having a 30% chance of surviving if transplanted. Results: Subjects answering the retransplantation survey gave a higher percentage of organs to the better prognostic group than subjects answering the blood marker survey, with a mean of 71.6% versus 65.0%, although this difference fell just short of statistical significance (P = 0.0581). Retransplantation survey respondents were significantly less likely to want to ignore prognostic information than were blood marker respondents (P = 0.026). Subjects in both survey groups were equally unwilling to abandon the poor prognostic group, with only 18% in each group choosing to give all the available organs to the better prognostic group. Conclusions: Respondents reacted more strongly to prognostic differences when they were due to retransplant status than to the results of a blood test, However, most people were not solely interested in the aggregate medical benefit brought by different allocation systems, but were also interested in the amount of benefit brought to the worst off. C1 CARNEGIE MELLON UNIV,DEPT SOCIAL & DECIS SCI,PHILADELPHIA,PA. RP UBEL, PA (reprint author), VET AFFAIRS MED CTR,UNIV & WOODLAND AVE,PHILADELPHIA,PA 19072, USA. NR 12 TC 32 Z9 32 U1 0 U2 2 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0168-8510 J9 HEALTH POLICY JI Health Policy PD NOV PY 1995 VL 34 IS 2 BP 145 EP 151 DI 10.1016/0168-8510(95)00714-4 PG 7 WC Health Care Sciences & Services; Health Policy & Services SC Health Care Sciences & Services GA TE821 UT WOS:A1995TE82100005 PM 10153483 ER PT J AU SIEBERT, JD AMBINDER, RF NAPOLI, VM QUINTANILLAMARTINEZ, L BANKS, PM GULLEY, ML AF SIEBERT, JD AMBINDER, RF NAPOLI, VM QUINTANILLAMARTINEZ, L BANKS, PM GULLEY, ML TI HUMAN IMMUNODEFICIENCY VIRUS-ASSOCIATED HODGKINS-DISEASE CONTAINS LATENT, NOT REPLICATIVE, EPSTEIN-BARR-VIRUS SO HUMAN PATHOLOGY LA English DT Article DE IMMUNODEFICIENCY; HODGKINS DISEASE; HUMAN IMMUNODEFICIENCY VIRUS; EPSTEIN-BARR VIRUS; LYMPHOPROLIFERATIVE DISORDER ID REED-STERNBERG CELLS; RAJI CELLS; VIRAL-DNA; AIDS; EXPRESSION; INFECTION; TRANSACTIVATOR; LYMPHOMAS; GENE; DISORDERS AB Severe immunodeficiency is associated with reactivation of latent Epstein-Barr virus (EBV) that is manifested by virus replication. It is unknown whether EBV replication also occurs in the Hodgkin's disease (HD) tissue of patients infected with the human immunodeficiency virus (HIV). Therefore, we studied paraffin-embedded lymph nodes from 13 cases of HIV-associated HD to determine the latent or replicative state of EBV infection. All patients were seropositive HIV-infected men; additional clinical information was available for 12 patients. The risk factor(s) for HIV infection were homosexuality (n = 7), intravenous drug abuse (n = 2), homosexuality and intravenous drug abuse (n = 1), sexual promiscuity (n = 1), or hemophilia (n = 1). Advanced clinical stage and B symptoms were common at the time of initial diagnosis of HD. The histological subtype of Hodgkin's disease was universally mixed cellularity, except for a single case classified as nodular sclerosis. Seven cases exhibited foci of relative lymphoid depletion. Five cases contained foci of necrosis. Reed-Sternberg (RS) cells and RS cell variants were positive for CD30/BerH2 and negative for CD45/LCA CD45RO/UCHL1, and CD20/L26 in all cases. Tumor cells were positive for CD15/LeuM1 in seven cases. In all 13 cases, RS cells and RS cell variants were infected by latent EBV as shown by in situ hybridization to EBV-encoded ribonucleic acid (EBER1). In 12 of 13 cases neoplastic cells coexpressed EBV latent membrane protein 1 (LMP1). EBV replication was examined by two different methods: immunohistochemistry to identify EBV-encoded BZLF1 protein and in situ hybridization to detect EBV BHLF1 transcripts. No positivity in RS or RS cell variants was detected with either assay of EBV replication (95% confidence interval [Cl] = 0% to 23%). The findings confirm that EBV is detected more frequently in HIV-associated HD when compared with immunocompetent patients with HD. The findings also suggest that EBV is tightly latent within RS and RS cell variants of HN-associated HD. It appears that factors other than host immune status are important in maintaining EBV latency in HIV-associated HD. HUM PATHOL 26:1191-1195. This is a US government work. There are no restrictions on its use. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. JOHNS HOPKINS ONCOL CTR,DEPT PATHOL,BALTIMORE,MD. EMORY UNIV,SCH MED,DEPT PATHOL,ATLANTA,GA 30322. GRADY MEM HOSP,DEPT PATHOL,ATLANTA,GA 30335. INST NACL NUTR,DEPT PATHOL,TLALPAN,MEXICO. FU NCI NIH HHS [K08-CA01615, R01 CA 55529, R03 CA 62696] NR 34 TC 26 Z9 27 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD NOV PY 1995 VL 26 IS 11 BP 1191 EP 1195 DI 10.1016/0046-8177(95)90192-2 PG 5 WC Pathology SC Pathology GA TD654 UT WOS:A1995TD65400005 PM 7590691 ER PT J AU GULLEY, ML AMIN, MB NICHOLLS, JM BANKS, PM AYALA, AG SRIGLEY, JR EAGAN, PA RO, JY AF GULLEY, ML AMIN, MB NICHOLLS, JM BANKS, PM AYALA, AG SRIGLEY, JR EAGAN, PA RO, JY TI EPSTEIN-BARR-VIRUS IS DETECTED IN UNDIFFERENTIATED NASOPHARYNGEAL CARCINOMA BUT NOT IN LYMPHOEPITHELIOMA-LIKE CARCINOMA OF THE URINARY-BLADDER SO HUMAN PATHOLOGY LA English DT Article DE EPSTEIN-BARR VIRUS; NASOPHARYNGEAL CARCINOMA; BLADDER CARCINOMA; LYMPHOEPITHELIOMA-LIKE CARCINOMA ID LATENT MEMBRANE-PROTEIN; INSITU HYBRIDIZATION; DNA AMPLIFICATION; CHINESE PATIENTS; EXPRESSION; CELLS; ASSOCIATION; INFECTION; SPECIMENS; GENOMES AB The Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC) and with lymphoepithelioma-like carcinomas developing-in certain anatomic sites. In this study, an in situ hybridization was used to identify EBV-encoded ribonucleic acid (RNA) (EBER1) transcripts in 32 of 45 cases of NPC but not in any of the 11 lymphoepithelioma-like carcinomas developing in the urinary bladder. EBER1 was most commonly detected in those NPCs having undifferentiated or nonkeratinizing squamous histology rather than the keratinizing squamous cell subtype of NPC. The EBV-encoded latent membrane protein I (LMP1) was expressed focally in only seven of 21 EBER1-positive NPCs by an immunohistochemical technique. These findings imply that EBER1 hybridization is more sensitive than LMP1 immunohistochemistry on paraffin sections in detecting carcinoma-associated virus. Previous in vitro studies have suggested that LMP1-expression might be a function of differentiation, but this study-of naturally infected NPCs showed no strong correlation between LMP1 positivity and degree of tumor differentiation, albeit a limited spectrum of differentiation that could be examined. In two cases in which frozen tissue was available, the NPCs were monoclonal with respect to viral DNA structure, implying that the virus was present before malignant transformation. Unlike NPCs, the lymphoepithelioma-like carcinomas of the bladder were uniformly EBV negative, lending further evidence to the growing body of literature linking EBV with lymphoepithelial carcinomas of foregut-derived tissues but not with similar-appearing tumors developing in other anatomic sites. HUM PATHOL 26:1207-1214. This is a US government work. There are no restrictions on its use. C1 AUDIE L MURPHY MEM VET ADM MED CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. HENRY FORD HOSP,DEPT PATHOL,DETROIT,MI 48202. UNIV HONG KONG,DEPT PATHOL,HONG KONG,HONG KONG. UNIV TEXAS,MD ANDERSON CANC CTR,DEPT PATHOL,HOUSTON,TX 77030. SUNNYBROOK MED CTR,DEPT PATHOL,TORONTO,ON M4N 3M5,CANADA. RP GULLEY, ML (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NCI NIH HHS [K08-CA01615] NR 55 TC 63 Z9 65 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD NOV PY 1995 VL 26 IS 11 BP 1207 EP 1214 DI 10.1016/0046-8177(95)90195-7 PG 8 WC Pathology SC Pathology GA TD654 UT WOS:A1995TD65400008 PM 7590694 ER PT J AU SMIR, BN RAMAIKA, CA CHO, CG GULLEY, ML AF SMIR, BN RAMAIKA, CA CHO, CG GULLEY, ML TI MOLECULAR EVIDENCE LINKS LYMPHOMATOUS POLYPOSIS OF THE GASTROINTESTINAL-TRACT WITH MANTLE CELL LYMPHOMA SO HUMAN PATHOLOGY LA English DT Note DE NON-HODGKINS LYMPHOMA; GASTROINTESTINAL POLYP; POLYPOSIS; BCL-1 ID LYMPHOCYTIC LYMPHOMA; CENTROCYTIC LYMPHOMA; ZONE LYMPHOMA; INTERMEDIATE DIFFERENTIATION; REARRANGEMENTS; DIAGNOSIS; GENE AB Lymphomatous polyposis (LP) is a subtype of non-Hodgkin's lymphoma manifested by numerous polyps affecting long segments of the gastrointestinal tract. The malignant cells of LP often share morphological and immunophenotypic similarity with cells of nodal-based mantle cell lymphoma. Recent genetic studies have shown that mantle cell lymphomas frequently possess a characteristic translocation of the JH/bcl-1 loci. In this study, polymerase chain reaction (PCR) and Southern blot analysis were used to show the presence of JH/bcl-1 translocation in a typical case of LP of the gastrointestinal tract, This provides strong molecular evidence for a biologic link between LP and mantle cell lymphoma. The findings also imply that detection of this translocation may be useful in the diagnosis of morphologically equivocal gastrointestinal biopsy specimens. HUM PATHOL 26:1282-1285. This is a US government work. There are no restrictions on its use. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 23 TC 12 Z9 12 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD NOV PY 1995 VL 26 IS 11 BP 1282 EP 1285 DI 10.1016/0046-8177(95)90207-4 PG 4 WC Pathology SC Pathology GA TD654 UT WOS:A1995TD65400020 PM 7590706 ER PT J AU DAVDA, RK STEPNIAKOWSKI, KT LU, G ULLIAN, ME GOODFRIEND, TL EGAN, BM AF DAVDA, RK STEPNIAKOWSKI, KT LU, G ULLIAN, ME GOODFRIEND, TL EGAN, BM TI OLEIC-ACID INHIBITS ENDOTHELIAL NITRIC-OXIDE SYNTHASE BY A PROTEIN-KINASE C-INDEPENDENT MECHANISM SO HYPERTENSION LA English DT Article DE FATTY ACIDS, NONESTERIFIED; NITRIC OXIDE; ENDOTHELIUM, VASCULAR; ENDOTHELIUM-DERIVED RELAXING FACTOR; HYPERTENSION, OBESITY ID FATTY-ACIDS; VASCULAR-RESPONSE; NORMOTENSIVE MEN; ACTIVATION; INSULIN; ACETYLCHOLINE; METABOLISM; CELLS; RESISTANCE; RELAXATION AB Many obese hypertensive individuals have a cluster of cardiovascular risk factors. This cluster includes plasma nonesterified fatty acid concentrations and turnover rates that are higher and more resistant to suppression by insulin than in lean and obese normotensive individuals. The higher fatty acids may contribute to cardiovascular risk in these patients by inhibiting endothelial cell nitric oxide synthase activity. To test this hypothesis, we quantified the effects of oleic (18:1[cis]) and other 18-carbon fatty acids on nitric oxide synthase activity in cultured bovine pulmonary artery endothelial cells by measuring the conversion of [H-3]L-arginine to [H-3]L-citrulline. Oleic acid (from 10 to 100 mu mol/L) caused a concentration-dependent decrease in nitric oxide synthase activity at baseline and during ATP and ionomycin (Ca2+ ionophore) stimulation. At 100 mu mol/L, linoleic (18:2[cis]) and oleic acids caused similar reductions of nitric oxide synthase activity, whereas elaidic (18:1[trans]) and stearic (18:0) acids had no effect. Oleic acid also inhibited the endothelium-dependent vasodilator response to acetylcholine in rabbit femoral artery rings preconstricted with phenylephrine (P<.05) but had no effect on the response to nitroprusside. The pattern of 18-carbon fatty acid effects on nitric oxide synthase activity in endothelial cells is consistent with activation of protein kinase C. Although oleic acid increased protein kinase C activity in endothelial cells, neither depletion of protein kinase C by 24-hour pretreatment with phorbol 12-myristate 13-acetate nor its inhibition with staurosporine eliminated the inhibitory effect of oleic acid on nitric oxide synthase. The vascular ring studies further indicate that oleic acid reduces the response to acetylcholine by inhibiting nitric oxide synthase activity rather than reducing the activation of guanylate cyclase or the effects of cGMP. Thus, elevated oleic acid values in obese hypertensive individuals may contribute to impaired endothelium-dependent vasodilation by a protein kinase C-independent mechanism. C1 MED UNIV S CAROLINA,DEPT PHARMACOL,DIV CLIN PHARMACOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT MED,DIV NEPHROL,CHARLESTON,SC 29425. UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT PHARMACOL,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU PHS HHS [R01-43164] NR 45 TC 160 Z9 170 U1 0 U2 2 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD NOV PY 1995 VL 26 IS 5 BP 764 EP 770 PG 7 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TD294 UT WOS:A1995TD29400008 PM 7591016 ER PT J AU WILLIAMS, JW AF WILLIAMS, JW TI 3 VS 10 DAYS OF TRIMETHOPRIM-SULFAMETHOXAZOLE FOR ACUTE MAXILLARY SINUSITIS - REPLY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter RP WILLIAMS, JW (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. RI Williams, Jr., John/A-3696-2008 OI Williams, Jr., John/0000-0002-5267-5558 NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD NOV 1 PY 1995 VL 274 IS 17 BP 1341 EP 1342 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA TB278 UT WOS:A1995TB27800010 ER PT J AU PETROF, BJ GOTTFRIED, SB EBY, J LAMANCA, J LEVINE, S AF PETROF, BJ GOTTFRIED, SB EBY, J LAMANCA, J LEVINE, S TI GROWTH-HORMONE DOES NOT PREVENT CORTICOSTEROID-INDUCED CHANGES IN RAT DIAPHRAGM STRUCTURE AND FUNCTION SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE MYOSIN HEAVY CHAIN; FIBER TYPES; ATROPHY ID OBSTRUCTIVE PULMONARY-DISEASE; POSITIVE NITROGEN-BALANCE; MUSCLE-FIBER SIZE; FACTOR-I; CONTRACTILE PROPERTIES; MYOSIN PHENOTYPE; SDH ACTIVITY; MYOPATHY; HUMANS; BIOCHEMISTRY AB The present study tested the hypothesis that growth hormone (GH), an anabolic agent, could prevent the abnormalities of diaphragm structure and function associated with short-term administration of the corticosteroid triamcinolone (TR). During a 10-day period, male rats (n = 33) were assigned to control (CTL), TR (1 mg . kg(-1). day(-1) im), and TR-GH (2 mg . kg(-1). day(-1) im) groups. Diaphragm weight was significantly reduced in the TR and TR-GH animals compared with the CTL animals, but there was no difference in the diaphragm-to-body weight ratio. Fiber type (I, IIa, and IIx/b) proportions did not differ among the three groups. However, in TR rats there was a significant reduction in the contribution of type IIx/b fibers to total diaphragm cross-sectional area due to marked atrophy (similar to 42% decrease in mean fiber cross-sectional area). There was no significant reversal of TR-induced type IIx/b fiber atrophy by concomitant GH administration. TR and TR-GH groups both exhibited a leftward shift of the force-frequency relationship and enhanced in vitro fatigue resistance, whereas maximal specific force was unaltered. We conclude that GH does not prevent corticosteroid-induced effects on the diaphragm under these conditions, possibly as a result of reduced nutritional intake associated with TR administration. C1 MCGILL UNIV,MONTREAL GEN HOSP,MONTREAL,PQ H3A 1A1,CANADA. MED COLL PENN,VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP PETROF, BJ (reprint author), ROYAL VICTORIA HOSP,DIV RESP,MEAKINS CHRISTIE LABS,RM L408,687 PINE AVE W,MONTREAL,PQ H3A 1A1,CANADA. NR 39 TC 8 Z9 8 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD NOV PY 1995 VL 79 IS 5 BP 1571 EP 1577 PG 7 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA TD929 UT WOS:A1995TD92900025 PM 8594016 ER PT J AU SCHWEITZER, M ASCH, DA AF SCHWEITZER, M ASCH, DA TI TIMING PAYMENTS TO SUBJECTS OF MAIL SURVEYS - COST-EFFECTIVENESS AND BIAS SO JOURNAL OF CLINICAL EPIDEMIOLOGY LA English DT Article DE COST-EFFECTIVENESS; DATA COLLECTION; EPIDEMIOLOGY; FINANCIAL INCENTIVES; HEALTH SERVICES RESEARCH; MAILED SURVEY; RESEARCH DESIGN; RESPONSE BIAS; SURVEY METHODS AB Although mailed surveys are an important component of epidemiological research, results from mailed surveys are often suspect because of poor response rates and the potential for nonresponse bias. Previous work has demonstrated that paying subjects to complete questionnaires increases response rates, but this work has not well addressed the impact of the timing of incentives on total cost, cost effectiveness, and response bias. We surveyed 400 university employees about health benefits. By random allocation, half received a check for $5 along with the mailed survey, and the other half received the promise of $5 on return of a completed survey. The response rates for both groups were about the same (64 and 59%, respectively), but prepayment was less expensive in aggregate and less expensive per response. In addition, we found that subjects with lower salaries were more likely to respond when paid in advance. We conclude that prepayment may actually be less expensive and more cost effective than payment on completion, but that the timing of payment may influence the profile of respondents. C1 UNIV PENN,SCH MED,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. UNIV PENN,WHARTON SCH,DEPT OPERAT & INFORMAT MANAGEMENT,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,GEN INTERNAL MED SECT,PHILADELPHIA,PA. NR 9 TC 28 Z9 27 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0895-4356 J9 J CLIN EPIDEMIOL JI J. Clin. Epidemiol. PD NOV PY 1995 VL 48 IS 11 BP 1325 EP 1329 DI 10.1016/0895-4356(95)00040-2 PG 5 WC Health Care Sciences & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TJ075 UT WOS:A1995TJ07500005 PM 7490595 ER PT J AU SKARE, JT SHANG, ES FOLEY, DM BLANCO, DR CHAMPION, CI MIRZABEKOV, T SOKOLOV, Y KAGAN, BL MILLER, JN LOVETT, MA AF SKARE, JT SHANG, ES FOLEY, DM BLANCO, DR CHAMPION, CI MIRZABEKOV, T SOKOLOV, Y KAGAN, BL MILLER, JN LOVETT, MA TI VIRULENT-STRAIN ASSOCIATED OUTER-MEMBRANE PROTEINS OF BORRELIA-BURGDORFERI SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE LYME BORRELIOSIS; LYME DISEASE; OUTER MEMBRANE PROTEIN; PORIN; SPIROCHETE ID LYME-DISEASE; TREPONEMA-PALLIDUM; ESCHERICHIA-COLI; SURFACE PROTEIN; YERSINIA-ENTEROCOLITICA; MOLECULAR ANALYSIS; EPITHELIAL-CELLS; SALMONELLA-TYPHIMURIUM; NEISSERIA-GONORRHOEAE; NORTH-AMERICAN AB We have isolated and purified outer membrane vesicles (OMV) from Borrelia burgdorferi strain B31 based on methods developed for isolation of Treponema pallidum OMV. Purified OMV exhibited distinct porin activities with conductances of 0.6 and 12.6 nano-Siemen and had no detectable beta-NADH oxidase activity indicating their outer membrane origin and their lack of inner membrane contamination, respectively. Hydrophobic proteins were identified by phase partitioning with Triton X-114. Most of these hydrophobic membrane proteins were not acylated, suggesting that they are outer membrane-spanning proteins. Identification of pamitate-labeled lipoproteins revealed that several were enriched in the OMV, several were enriched in the protoplasmic cylinder inner membrane fraction, and others were found exclusively associated with the inner membrane. The protein composition of OMV changed significantly with successive in vitro cultivation of strain B31, Using antiserum with specificity for virulent strain B31, we identified OMV antigens on the surface of the spirochete and identified proteins whose presence in OMV could be correlated with virulence and protective immunity in the rabbit Lyme disease model. These virulent strain associated outer membrane-spanning proteins may provide new insight into the pathogenesis of Lyme disease. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV INFECT DIS,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,INST NEUROPSYCHIAT,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP SKARE, JT (reprint author), UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,10833 LE CONTE AVE,LOS ANGELES,CA 90024, USA. FU NIAID NIH HHS [AI-21352, AI-29733, AI-12601] NR 68 TC 71 Z9 71 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD NOV PY 1995 VL 96 IS 5 BP 2380 EP 2392 DI 10.1172/JCI118295 PG 13 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA TC979 UT WOS:A1995TC97900037 PM 7593626 ER PT J AU LIN, T MATSUZAKI, G UMESUE, M OMOTO, K YOSHIDA, H HARADA, M SINGARAM, C HIROMATSU, K NOMOTO, K AF LIN, T MATSUZAKI, G UMESUE, M OMOTO, K YOSHIDA, H HARADA, M SINGARAM, C HIROMATSU, K NOMOTO, K TI DEVELOPMENT OF TCR-GAMMA-DELTA CD4(-)CD8(+)ALPHA-ALPHA BUT NOT TCR-ALPHA-BETA CD4(-)CD8(+)ALPHA-ALPHA I-IEL IS RESISTANT TO CYCLOSPORINE-A SO JOURNAL OF IMMUNOLOGY LA English DT Article ID T-CELL RECEPTOR; INTESTINAL INTRAEPITHELIAL LYMPHOCYTES; MONOCLONAL-ANTIBODY; MICE; DIFFERENTIATION; THYMUS; LYMPHOPOIESIS; EPITHELIUM; SELECTION; THYMOCYTES AB Present evidence suggests that cyclosporin A (CSA) inhibits the development of both alpha beta and gamma delta T cells in the thymus. However, whether CSA can inhibit the development of murine intestinal intraepithelial lymphocytes (i-IEL) is unknown as most i-IEL are clearly derived from a different lineage than the conventional thymus-derived T cells found in the periphery. Using the adult thymectomized, lethally irradiated bone-marrow reconstituted chimera (ATXBM mice) as a model for the development of extrathymically derived i-IEL and the fetal thymus-grafted (FTG) nude mice as a model for the development of thymically derived i-IEL, we demonstrate that CSA nearly completely inhibited the development of extrathymically, and possibly thymically, derived TCR-alpha beta i-IEL. Most of the TCR-alpha beta i-IEL whose development was inhibited by CSA belonged to the CD4(-)CD8(+) alpha alpha subset. In contrast, the development of extrathymically and thymically derived TCR-gamma delta i-IEL was completely resistant to CSA. The phenotype of CSA-resistant TCR-gamma delta i-IEL in these models was not different from those in control mice, and the TCR-gamma delta i-IEL in CSA-treated mice appear to be mature and activated as most were large, granular, and CD69(+). Lastly, we demonstrate that CSA does not affect the extrathymic positive selection of V delta 4 i-IEL in C3H hosts. These results suggest that despite their similarity, the intracellular activation cascade involved after TCR stimulation between TCR-alpha beta CD4(-)CD8(+) alpha alpha and TCR-gamma delta CD4(-)CD8(+)alpha alpha i-IEL are markedly different. C1 UNIV WISCONSIN HOSP,DEPT MED,GASTROENTEROL SECT,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. KYUSHU UNIV,MED INST BIOREGULAT,DEPT IMMUNOL,FUKUOKA 812,JAPAN. NAGOYA UNIV,SCH MED,DIS MECHANISM & CONTROL RES INST,GERMFREE LIFE LAB,NAGOYA,AICHI 466,JAPAN. NR 39 TC 15 Z9 15 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD NOV 1 PY 1995 VL 155 IS 9 BP 4224 EP 4230 PG 7 WC Immunology SC Immunology GA TB468 UT WOS:A1995TB46800013 PM 7594578 ER PT J AU Correa, AL Velez, G Albert, M Luther, M Rinaldi, MG Graybill, JR AF Correa, AL Velez, G Albert, M Luther, M Rinaldi, MG Graybill, JR TI Comparison of D0870 and fluconazole in the treatment of murine cryptococcal meningitis SO JOURNAL OF MEDICAL AND VETERINARY MYCOLOGY LA English DT Article ID ACQUIRED-IMMUNODEFICIENCY-SYNDROME; AMPHOTERICIN-B; SUSCEPTIBILITY; INFECTIONS; AIDS AB Cryptococcal meningitis is a common infection in patients with AIDS. Using a murine cryptococcosis model, we compared treatment with a new triazole, D0870, and fluconazole. Groups of ICR (Institute for Cancer Research) mice were infected intracerebrally with eight different isolates of Cryptococcus neoformans variety neoformans with different in vitro susceptibilities to fluconazole. For survival studies mice were challenged with two to four times LD(50) or six to nine times LD(50). Treatment was given for 10 days. Mice were observed through to day 30. To assess the effect of treatment on fungal tissue burden, mice received a three to five times LD(50) inoculum and treatment for 10 days. They were sacrificed on day 12 and serial dilutions of brain homogenates were cultured. Fluconazole prolonged survival primarily in isolates which were susceptible in vitro. D0870 prolonged survival in all isolates except one, which was also resistant in vitro to D0870 and fluconazole. Both drugs reduced colony counts of all isolates. D0870 warrants further development for use in cryptococcosis, and appears effective for isolates relatively resistant to fluconazole. There is a relative correlation of in vivo and in vitro susceptibility to D0870 as well as fluconazole. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. RP Correa, AL (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,DIV INFECT DIS 111F,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 17 TC 6 Z9 6 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0268-1218 J9 J MED VET MYCOL JI J. Med. Vet. Mycol. PD NOV-DEC PY 1995 VL 33 IS 6 BP 367 EP 374 PG 8 WC Mycology SC Mycology GA TZ017 UT WOS:A1995TZ01700002 PM 8683404 ER PT J AU CARDIN, S SOLL, AH TACHE, Y AF CARDIN, S SOLL, AH TACHE, Y TI DIFFERENT EFFECTS OF INDOMETHACIN AND NABUMETONE ON PROSTAGLANDIN-MEDIATED GASTRIC RESPONSES TO CENTRAL VAGAL ACTIVATION IN RATS SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID THYROTROPIN-RELEASING-HORMONE; ACTIVE METABOLITE; 6-METHOXY-2-NAPHTHYLACETIC ACID; LESION FORMATION; PHARMACOLOGY; 6MNA AB Intracisternal injection of a stable thyrotropin-releasing hormone (TRH) analog increases gastric prostaglandins release and mucosal resistance to injury through central vagal pathways. The effects of two nonsteroidal anti-inflammatory drugs, indomethacin (INDO) and nabumetone on intracisternal injection of various doses of TRH-induced gastric acid secretion and changes in mucosal resistance were investigated in urethane-anesthetized rats. Doses of INDO (5 mg/kg) and nabumetone (13.75 mg/kg) producing similar acute anti-inflammatory response in the carrageenin-induced paw edema were injected i.p. in all studies. INDO potentiated the acid secretion induced by intracisternal injection of TRH at 25, 50 and 200 ng by 5.1-, 1.9- and 1.4-fold, respectively, whereas nabumetone did not modify the secretory response to TRH. Moderate erosions were observed in 100% of rats treated with the combination of INDO and TRH (200 ng) whereas no erosions were observed when TRH or INDO were given alone or TRH in combination with nabumetone. TRH at 7 ng reduced mucosal damage induced by intragastric administration of ethanol (60%, 1 ml/kg) by 63%. The mucosal protective action of TRH was abolished by INDO but not altered by nabumetone pretreatment. These data indicate that at comparable anti-inflammatory doses, nabumetone, unlike INDO, neither blocks the protection against ethanol injury induced by low doses of TRH injected intracisternally nor potentiates the gastric acid secretion or lesions induced by higher dose of TRH. We speculate that these differences reflect reduced inhibition of gastric prostaglandins by nabumetone. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE DIGEST DIS CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024. FU NIDDK NIH HHS [DK-30110]; NIMH NIH HHS [MH-00663] NR 26 TC 8 Z9 8 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD NOV PY 1995 VL 275 IS 2 BP 667 EP 673 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TE743 UT WOS:A1995TE74300019 PM 7473153 ER PT J AU Pinzur, MS Cox, W Kaiser, J Morris, T Patwardhan, A Vrbos, L AF Pinzur, MS Cox, W Kaiser, J Morris, T Patwardhan, A Vrbos, L TI The effect of prosthetic alignment on relative limb loading in persons with trans-tibial amputation: A preliminary report SO JOURNAL OF REHABILITATION RESEARCH AND DEVELOPMENT LA English DT Article DE below-knee amputation; gait analysis; ground reaction force; stance phase time ID AMPUTEES; GAIT AB The prosthetic sockets of 14 independent persons with unilateral trans-tibial (BK) amputation were mounted on an adjustable alignment pylon. Vertical ground reaction forces were recorded in neutral prosthetic alignment and in 10 degrees of prosthetic socket varus, valgus, flexion, and extension. Stance phase time, peak vertical ground reaction force, and impulse were all found to be increased on the sound limb when compared to the amputated residual limb. Significant differences were found in stance phase time and peak vertical ground reaction force when comparing malaligned with neutrally aligned prosthetic limbs. Significant differences were also seen in impulse between neutrally aligned and malaligned prosthetic limbs. The results suggest that prosthetic malalignment in persons with trans-tibial amputation leads to increased loading of the contralateral limb. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,REHABIL ENGN CTR,HINES,IL 60141. SCHECK & SIRESS ORTHOT & PROSTHET INC,OAK PK,IL 60302. RP Pinzur, MS (reprint author), LOYOLA UNIV,MED CTR,DEPT ORTHOPAED SURG,2160 S 1ST AVE,MAYWOOD,IL 60153, USA. NR 11 TC 24 Z9 28 U1 1 U2 5 PU JOURNAL REHAB RES & DEV PI BALTIMORE PA DEPT OF VETERANS AFFAIRS REHABIL RES & DEVELOP CTR 103 SOUTH GAY STREET, BALTIMORE, MD 21202-4051 SN 0007-506X J9 J REHABIL RES DEV JI J. Rehabil. Res. Dev. PD NOV PY 1995 VL 32 IS 4 BP 373 EP 377 PG 5 WC Rehabilitation SC Rehabilitation GA TN835 UT WOS:A1995TN83500009 PM 8770802 ER PT J AU YEAGER, RA MONETA, GL EDWARDS, JM TAYLOR, LM MCCONNELL, DB PORTER, JM AF YEAGER, RA MONETA, GL EDWARDS, JM TAYLOR, LM MCCONNELL, DB PORTER, JM TI DEEP-VEIN THROMBOSIS ASSOCIATED WITH LOWER-EXTREMITY AMPUTATION SO JOURNAL OF VASCULAR SURGERY LA English DT Article; Proceedings Paper CT 7th Annual Meeting of the American-Venous-Forum CY FEB 23-25, 1995 CL FT LAUDERDALE, FL SP Amer Venous Forum ID VENOUS THROMBOEMBOLISM; COAGULATION AB Purpose: Patients undergoing lower extremity amputation are perceived to be at high risk for deep vein thrombosis (DVT). Limited data are available, however, to confirm this impression. The purpose of this study is to prospectively document the incidence of DVT complicating lower extremity amputation. Methods: During a recent 28-month period, 72 patients (71 men, 1 woman; mean age 68 years) undergoing major lower extremity amputation (31 above-knee and 41 below-knee) were prospectively evaluated with perioperative duplex scanning for DVT. Results: DVT was documented in nine (12.5%) patients (one bilateral, four ipsilateral, and four contralateral to amputation). Patients with a history of venous disease were at significantly higher risk for development of DVT (p = 0.02). Thrombi were located at or proximal to the popliteal vein in eight patients and were isolated to the tibial veins in one patient; DVT was identified before operation in six patients and after operation in three. Patients with DVT were treated with heparin anticoagulation, with no patient experiencing clinical symptoms compatible with pulmonary embolism. Conclusions: In our recent experience, lower extremity amputation is associated with DVT at or proximal to the popliteal vein in 11% of patients. Documentation of DVT prevalence is essential to assist surgeons in planning a management strategy for prevention, diagnosis, and treatment of DVT associated with lower extremity amputation. C1 OREGON HLTH SCI UNIV,DEPT SURG,DIV VASC SURG,PORTLAND,OR 97201. RP YEAGER, RA (reprint author), PORTLAND VET AFFAIRS MED CTR,SURG SERV 112P,POB 1034,PORTLAND,OR 97207, USA. NR 17 TC 23 Z9 26 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0741-5214 J9 J VASC SURG JI J. Vasc. Surg. PD NOV PY 1995 VL 22 IS 5 BP 612 EP 615 DI 10.1016/S0741-5214(95)70048-X PG 4 WC Surgery; Peripheral Vascular Disease SC Surgery; Cardiovascular System & Cardiology GA TF306 UT WOS:A1995TF30600017 PM 7494364 ER PT J AU TYLER, KL SQUIER, MKT RODGERS, SE SCHNEIDER, BE OBERHAUS, SM GRDINA, TA COHEN, JJ DERMODY, TS AF TYLER, KL SQUIER, MKT RODGERS, SE SCHNEIDER, BE OBERHAUS, SM GRDINA, TA COHEN, JJ DERMODY, TS TI DIFFERENCES IN THE CAPACITY OF REOVIRUS STRAINS TO INDUCE APOPTOSIS ARE DETERMINED BY THE VIRAL ATTACHMENT PROTEIN SIGMA-1 SO JOURNAL OF VIROLOGY LA English DT Article ID PROGRAMMED CELL-DEATH; MOUSE L-CELLS; MAMMALIAN REOVIRUSES; SPECIFIED POLYPEPTIDES; MOLECULAR-BASIS; TRANSLATION PRODUCTS; SUBVIRION PARTICLES; DNA FRAGMENTATION; RNA SEGMENTS; E1A PROTEINS AB Reoviruses are important models for studies of viral pathogenesis; however, the mechanisms by which these viruses produce cytopathic effects in infected cells have not been defined, In this report, we show that murine L929 (L) cells infected with prototype reovirus strains type 1 Lang (T1L) and type 3 Dearing (T3D) undergo apoptosis and that T3D induces apoptosis to a substantially greater extent than T1L, Using T1L x T3D reassortant viruses, we found that differences in the capacity of T1L and T3D to induce apoptosis are determined by the viral S1 gene segment, which encodes the viral attachment protein al and the non-virion-associated protein sigma 1s. Apoptosis was induced by UV-inactivated, replication-incompetent reovirus virions, which do not contain sigma 1s and do not mediate its synthesis in infected cells, Additionally, T3D-induced apoptosis was inhibited by anti-reovirus monoclonal antibodies that inhibit no cell attachment and disassembly, These results indicate that sigma 1, rather than sigma 1s, is required for induction of apoptosis by the reovirus and suggest that interaction of virions with cell surface receptors is an essential step in this mechanism of cell killing. C1 UNIV COLORADO,HLTH SCI CTR,DEPT NEUROL,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DEPT MED,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DEPT MICROBIOL,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DEPT IMMUNOL,DENVER,CO 80220. DENVER VET AFFAIRS MED CTR,NEUROL SERV,DENVER,CO 80220. VANDERBILT UNIV,SCH MED,DEPT PEDIAT,NASHVILLE,TN 37232. VANDERBILT UNIV,SCH MED,DEPT IMMUNOL & MICROBIOL,NASHVILLE,TN 37232. VANDERBILT UNIV,SCH MED,ELIZABETH B LAMB CTR PEDIAT RES,NASHVILLE,TN 37232. OI Tyler, Kenneth/0000-0003-3294-5888 FU NIAID NIH HHS [AI32539] NR 81 TC 146 Z9 152 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD NOV PY 1995 VL 69 IS 11 BP 6972 EP 6979 PG 8 WC Virology SC Virology GA RZ100 UT WOS:A1995RZ10000048 PM 7474116 ER PT J AU CAO, J VESCIO, RA RETTIG, MB HONG, CH KIM, A LEE, JC LICHTENSTEIN, AK BERENSON, JR AF CAO, J VESCIO, RA RETTIG, MB HONG, CH KIM, A LEE, JC LICHTENSTEIN, AK BERENSON, JR TI A CD10-POSITIVE SUBSET OF MALIGNANT-CELLS IS IDENTIFIED IN MULTIPLE-MYELOMA USING PCR WITH PATIENT-SPECIFIC IMMUNOGLOBULIN GENE PRIMERS SO LEUKEMIA LA English DT Article DE CD10; PCR; MYELOMA; IMMUNOGLOBULIN; HEAVY-CHAIN VARIABLE REGION ID LYMPHOBLASTIC-LEUKEMIA ANTIGEN; PLASMA-CELLS; (CALLA)-POSITIVE MYELOMA; CIRCULATING LYMPHOCYTES; CLINICAL-FEATURES; PERIPHERAL-BLOOD; BONE-MARROW; CALLA; EXPRESSION; NEUTROPHILS AB Immunophenotypic studies show the presence of CD10-bearing malignant cells in a small subset of multiple myeloma (MM) patients. We used a sensitive PCR-based technique in order to determine the frequency that MM patients contain a malignant subpopulation which expresses this antigen. The immunoglobulin (Ig) heavy chain variable region (V-H) gene sequence expressed by the malignant clone in MM can be used as a tumor specific marker. After determining this sequence in six MM patients, patient specific V-H oligonucleotide primers from complementarity determining region (CDR) sequences were generated. Bone marrow mononuclear cells from these patients were incubated with two different anti-CD10 antibodies or isotype identical murine IgG controls. Cells were then sorted by flow cytometry into the 1% brightest cells containing >99.99% CD10-positive cells and two fractions including the 90 and 10% dimmest staining cells. PCR amplification was performed on DNA from approximately 10(4) cells (0.1 mu g) using patient specific CDR1 and CDR3 primers. Detectable PCR product was obtained in each sorted sample although the intensity of the band was much higher in cells lacking CD10 expression (the 90 and 10% dimmest fractions) than in the CD10-bearing (1% brightest) population. These results imply that there is a small population of CD10-bearing clonal cells in most, if not all patients with MM. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,DIV HEMATOL ONCOL,LOS ANGELES,CA. JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90034. NR 43 TC 9 Z9 9 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0887-6924 J9 LEUKEMIA JI Leukemia PD NOV PY 1995 VL 9 IS 11 BP 1948 EP 1953 PG 6 WC Oncology; Hematology SC Oncology; Hematology GA TG562 UT WOS:A1995TG56200024 PM 7475288 ER PT J AU WENZEL, SL BAKHTIAR, L CASKEY, NH HARDIE, E REDFORD, C SADLER, N GELBERG, L AF WENZEL, SL BAKHTIAR, L CASKEY, NH HARDIE, E REDFORD, C SADLER, N GELBERG, L TI HOMELESS VETERANS UTILIZATION OF MEDICAL, PSYCHIATRIC, AND SUBSTANCE-ABUSE SERVICES SO MEDICAL CARE LA English DT Article DE HOMELESS VETERANS; SERVICE UTILIZATION ID MENTAL-HEALTH; CARE; ADJUSTMENT; ALCOHOL; PROGRAM; ADULTS; WOMEN AB This study focuses on the association between homeless veterans' prior utilization of medical, psychiatric, and substance abuse services and biopsychosocial characteristics reported at admission into a domiciliary care program. Given the large number of veterans in the US homeless population and their health care needs, understanding factors associated with health service use among homeless veterans is significant. Research participants were 429 homeless male veterans who had been admitted to the Domiciliary Care for Homeless Veterans Program site at the West Los Angeles Veterans Affairs Medical Center between February 1988 and July 1992 for treatment of medical, psychiatric, or substance disorders. Results of logistic regression analyses indicated that self-reported need (chronic medical problems, serious psychiatric symptoms, combat stress, alcohol use) and evaluated need for care (evidence of liver dysfunction) were important to veterans' use of health services in the 6 months before program admission. Predisposing social structure factors (education, residential stability, and usual sleeping place) were also significant predictors of service utilization. Overall, need factors were more strongly related to service use. Supplementary logistic regression analyses indicated that comorbidity of need factors deserves attention in understanding homeless veterans' use of services. In conclusion, it is important to attend to predisposing social structure factors as potential barriers to care for homeless veterans. C1 RAND CORP,SANTA MONICA,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP WENZEL, SL (reprint author), UNIV CALIF LOS ANGELES,DIV FAMILY MED,50-071 CHS,BOX 951683,LOS ANGELES,CA 90095, USA. NR 47 TC 46 Z9 46 U1 1 U2 5 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD NOV PY 1995 VL 33 IS 11 BP 1132 EP 1144 DI 10.1097/00005650-199511000-00006 PG 13 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TE669 UT WOS:A1995TE66900006 PM 7475422 ER PT J AU VALENTE, AJ ABRAMOVA, MA PEARSON, DW CLARK, JR CLARK, RA AF VALENTE, AJ ABRAMOVA, MA PEARSON, DW CLARK, JR CLARK, RA TI TRANSCRIPTIONAL REGULATION OF THE CALRETICULIN GENE IN THE HL-60 MYELOID CELL-LINE SO MOLECULAR BIOLOGY OF THE CELL LA English DT Meeting Abstract C1 AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CELL BIOL PI BETHESDA PA PUBL OFFICE 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD NOV PY 1995 VL 6 SU S BP 2028 EP 2028 PG 1 WC Cell Biology SC Cell Biology GA TF513 UT WOS:A1995TF51302025 ER PT J AU BROOKS, MJ MELNIK, G AF BROOKS, MJ MELNIK, G TI THE REFEEDING SYNDROME - AN APPROACH TO UNDERSTANDING ITS COMPLICATIONS AND PREVENTING ITS OCCURRENCE SO PHARMACOTHERAPY LA English DT Review ID TOTAL PARENTERAL-NUTRITION; RECEIVING INTRAVENOUS HYPERALIMENTATION; ACUTE-RENAL-FAILURE; SEVERE HYPOPHOSPHATEMIA; ANOREXIA-NERVOSA; HEMOLYTIC-ANEMIA; WERNICKES ENCEPHALOPATHY; METABOLIC ABNORMALITIES; PHOSPHATE-DEPLETION; PATIENT AB The refeeding syndrome (RS) is a complication of nutritional support that potentially causes considerable morbidity and mortality. Compensatory metabolic alterations secondary to chronic starvation predispose malnourished patients to RS. Providing nutritional support initiates an intracellular shift of potassium, magnesium, and phosphate that results in many adverse effects. The literature addressing RS focuses on only one electrolyte abnormality, hypophosphatemia; however, often all three electrolyte levels are perturbed. Thus RS should be characterized as a syndrome of generalized fluid and electrolyte imbalance. Recommended electrolyte supplementation and laboratory monitoring can help prevent the disorder in susceptible patients. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PHARMACOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,DEPT PHARM,SAN ANTONIO,TX 78284. RP BROOKS, MJ (reprint author), APPL CLIN COMMUN INC,4 CENTURY DR,PARSIPPANY,NJ 07054, USA. NR 76 TC 63 Z9 70 U1 2 U2 8 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER BOX 806 171 HARRISON AVE, BOSTON, MA 02111 SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PD NOV-DEC PY 1995 VL 15 IS 6 BP 713 EP 726 PG 14 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TG174 UT WOS:A1995TG17400005 PM 8602378 ER PT J AU MOORE, JG COBURN, JW SANDERS, MC MCSORLEY, DJ SIRGO, MA AF MOORE, JG COBURN, JW SANDERS, MC MCSORLEY, DJ SIRGO, MA TI EFFECTS OF SUCRALFATE AND RANITIDINE ON ALUMINUM CONCENTRATIONS IN ELDERLY VOLUNTEERS SO PHARMACOTHERAPY LA English DT Article ID GASTROINTESTINAL ABSORPTION; RENAL-FAILURE; TOXICITY; DEFEROXAMINE; DISEASE; PATIENT AB Elevated aluminum concentrations have been implicated in several disease states in the elderly. We examined the effects of sucralfate, a basic aluminum salt of sucrose sulfate, and ranitidine, administered individually and in combination, on plasma and urine aluminum concentrations in the elderly in a prospective, randomized, three-arm crossover study. Subjects were 20 healthy volunteers over age 65 years, with no clinically significant comorbidities or recent use of aluminum-containing drugs or histamine (H)(2)-antagonists. The three regimens were ranitidine 300 mg at bedtime, sucralfate 1 g 4 times/day, and ranitidine 300 mg at bedtime plus sucralfate 1 g 4 times/day, administered for 4 weeks, with a washout period of at least 1 week between regimens. Plasma and urine aluminum concentrations were measured on days 0, 1, 7, 14, and 28 of each regimen. After 28 days, mean plasma aluminum concentrations were significantly higher in subjects receiving sucralfate alone (8.5 +/- 1.8 mu g/L) and sucralfate plus ranitidine (5.1 +/- 1.3 mu g/L) compared with those receiving ranitidine alone (2.4 +/- 0.7 mu g/L). Urine aluminum concentrations were significantly higher in subjects receiving sucralfate alone (133.2 +/- 32.8 mu g/g creatinine) and sucralfate plus ranitidine (148.1 +/- 51.9 mu g/g creatinine) compared with those receiving ranitidine alone (11.0 +/- 3.7 mu g/g creatinine). There was no significant difference in plasma or urine aluminum concentrations between subjects who received sucralfate alone versus those who received sucralfate plus ranitidine. Sucralfate 4 g/day in elderly subjects produces a significant increase in both plasma and urine aluminum concentrations, compared with ranitidine 300 mg/day. This increase most likely is secondary to gastrointestinal absorption of aluminum in the sucralfate formulation. The clinical relevance of this increase requires further evaluation. C1 GLAXO INC,RES TRIANGLE PK,NC 27709. VET AFFAIRS MED CTR,SALT LAKE CITY,UT 84148. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. NR 17 TC 6 Z9 8 U1 0 U2 0 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER BOX 806 171 HARRISON AVE, BOSTON, MA 02111 SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PD NOV-DEC PY 1995 VL 15 IS 6 BP 742 EP 746 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TG174 UT WOS:A1995TG17400009 PM 8602382 ER PT J AU GRAHAM, LS AF GRAHAM, LS TI THE AAPM/RSNA PHYSICS TUTORIAL FOR RESIDENTS - QUALITY-CONTROL FOR SPECT SYSTEMS SO RADIOGRAPHICS LA English DT Article; Proceedings Paper CT 1994 Radiological-Society-of-North-America Scientific Assembly CY NOV 27-DEC 02, 1994 CL CHICAGO, IL SP Radiol Soc N Amer DE EMISSION CT ECT; EMISSION CT ECT, QUALITY ASSURANCE; PHYSICS; QUALITY ASSURANCE ID TOMOGRAPHY AB A comprehensive set of acceptance tests is the basis for an effective quality control program. Acceptance tests indicate whether the scintillation camera meets published specifications but should also include evaluation of certain parameters not specified by the vendor but that can seriously affect image quality. Once a camera is ''accepted,'' initial quality control tests serve as a benchmark for future measurements, Tomographic systems must be subjected to the same basic quality control program as planar cameras. Flood field images must be acquired and evaluated daily; spatial resolution must be tested once each week, Less frequently, parameters such as multiple-window spatial registration, collimator uniformity, system sensitivity, and camera ''dead time'' should be evaluated, Single photon emission computed tomographic systems require additional tests to ensure optimum performance, Center-of-rotation calibration and verification of detector registration are needed to avoid losses of spatial resolution, Flood corrections based on high-count images eliminate residual detector nonuniformities and correct for subtle collimator defects. Pixel size must be calibrated to avoid errors in attenuation correction and distortion when cardiac and brain reorientation software is used. Completed clinical studies must be checked for unacceptable patient movement and incomplete views, A quality control Program may be time-consuming, but, from the standpoint of maximum benefit to patients and confidence in clinical interpretations; there is no alternative. C1 UNIV CALIF LOS ANGELES,SCH MED,DIV BIOMED PHYS,LOS ANGELES,CA. RP GRAHAM, LS (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,NEUTRON THERAPY BLDG 345,LOS ANGELES,CA 90073, USA. NR 35 TC 4 Z9 5 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0271-5333 J9 RADIOGRAPHICS JI Radiographics PD NOV PY 1995 VL 15 IS 6 BP 1471 EP 1481 PG 11 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TF402 UT WOS:A1995TF40200021 PM 8577970 ER PT J AU HYTIROGLOU, P THUNG, SN GERBER, MA AF HYTIROGLOU, P THUNG, SN GERBER, MA TI HISTOLOGICAL CLASSIFICATION AND QUANTITATION OF THE SEVERITY OF CHRONIC HEPATITIS - KEEP IT SIMPLE SO SEMINARS IN LIVER DISEASE LA English DT Review ID CHRONIC ACTIVE HEPATITIS; CHRONIC LIVER-DISEASE; VIRAL-HEPATITIS; NOMENCLATURE C1 CUNY,MT SINAI SCH MED,LILLIAN & HENRY M STRATTON HANS POPPER DEPT PATHO,NEW YORK,NY 10021. TULANE UNIV,SCH MED,DEPT PATHOL & LAB MED,NEW ORLEANS,LA 70112. RP HYTIROGLOU, P (reprint author), BRONX VET AFFAIRS MED CTR,DEPT PATHOL & LAB MED 113,130 W KINGSBRIDGE RD,BRONX,NY 10468, USA. NR 27 TC 47 Z9 47 U1 0 U2 0 PU THIEME MEDICAL PUBL INC PI NEW YORK PA 381 PARK AVE SOUTH, NEW YORK, NY 10016 SN 0272-8087 J9 SEMIN LIVER DIS JI Semin. Liver Dis. PD NOV PY 1995 VL 15 IS 4 BP 414 EP 421 DI 10.1055/s-2007-1007291 PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TD831 UT WOS:A1995TD83100009 PM 8578324 ER PT J AU MENG, QC BALCELLS, E DELLITALIA, L DURAND, J OPARIL, S AF MENG, QC BALCELLS, E DELLITALIA, L DURAND, J OPARIL, S TI SENSITIVE METHOD FOR QUANTITATION OF ANGIOTENSIN-CONVERTING ENZYME (ACE) ACTIVITY IN TISSUE SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE ANGIOTENSIN-CONVERTING ENZYME; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ANGIOTENSIN I; ANGIOTENSIN II; RENIN-ANGIOTENSIN SYSTEM ID MYOCARDIAL-INFARCTION; ASSAY; SERUM AB A novel sensitive and specific method for the measurement of tissue angiotensin-converting enzyme (ACE) activity utilizing HPLC is described. ACE activity was determined in detergent-extracted canine hearts utilizing the synthetic ACE-specific substrate hippuryl histidyl leucine (HHL), both in the presence and the absence of the site-specific inhibitor captopril. Tissue ACE activity was quantitated from the moles of hippuric acid (HA) formed, in time-fixed assays, utilizing HPLC separation of HA from HHL and UV-spectrophotometry for quantitation of HA as in the standard Cushman and Cheung assay (Cushman DW and Cheung HS, Biochem Pharmacol 20: 1637-1648, 1971). Separation of HA from HHL was performed by reverse phase HPLC on a phenyl silica gel column with an eluent consisting of 20% acetonitrile in 0.1 M aqueous ammonium phosphate buffer, pH 6.8. After the standard liquid/liquid extraction procedure with ethyl acetate, HPLC analysis revealed the presence of unreacted substrate, HHL, in amounts comparable to the product of interest, HA, in the final assay; moreover, the amount of HA formed did not fall completely to zero in the presence of captopril. Regional studies of canine cardiac ACE activity utilizing the HPLC-based assay and the standard assay method showed a significantly higher ACE activity in the right ventricle compared with the left ventricle (2.37 +/- 0.7 vs 1.24 +/- 0.18 mU/g, P < 0.05 [N = 6], respectively) in the HPLC-based assay, but no difference in right and left ventricular ACE activities by the standard assay (0.25 +/- 0.08 vs 0.31 +/- 0.09 mU/g [N = 6], respectively). Kinetic studies utilizing the HPLC-based assay coupled with the use of captopril showed K-m (1.34 +/- 0.08 mM) and V-max (36.8 +/- 11.5 x 10(-10) M/min) values in agreement with those in the literature. Our results demonstrate that the application of HPLC to the standard Cushman and Cheung assay improves the sensitivity and specificity of the standard assay and enables the use of much smaller amounts (similar to 4 vs similar to 400 mg for the Cushman and Cheung assay) of tissue for ACE activity assay. C1 UNIV ALABAMA,DEPT MED,DIV CARDIOVASC DIS,VASC BIOL & HYPERTENS PROGRAM,BIRMINGHAM,AL 35294. UNIV ALABAMA,BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL 35294. FU NHLBI NIH HHS [HL47081, HL37722, HL07457] NR 20 TC 47 Z9 47 U1 1 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD OCT 26 PY 1995 VL 50 IS 9 BP 1445 EP 1450 DI 10.1016/0006-2952(95)02038-1 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TG549 UT WOS:A1995TG54900017 PM 7503795 ER PT J AU WALD, TG SHULT, P KRAUSE, P MILLER, BA DRINKA, P GRAVENSTEIN, S AF WALD, TG SHULT, P KRAUSE, P MILLER, BA DRINKA, P GRAVENSTEIN, S TI A RHINOVIRUS OUTBREAK AMONG RESIDENTS OF A LONG-TERM-CARE FACILITY SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE RHINOVIRUS; NURSING HOMES; RESPIRATORY TRACT INFECTIONS; DISEASE OUTBREAKS; LUNG DISEASES, OBSTRUCTIVE ID ILLNESS; INFECTIONS; PRECIPITANTS; VIRUSES; ASTHMA AB Objective: To describe the epidemiology of and clinical findings associated with a rhinovirus outbreak that occurred among institutionalized elderly persons. Design: Retrospective review of medical records and nursing surveillance reports. Setting: A 685-bed, long-term care facility for veterans and their spouses. Patients: 33 persons from whom rhinovirus was cultured. Measurements: Throat and nasopharyngeal virus culture; review of medical records to determine under-lying diseases, signs and symptoms of respiratory illness, illness duration, and interventions during illness; and review of nursing surveillance reports to determine room locations of ill persons. Results: Between 14 August and 2 September 1993, the number of respiratory illnesses increased. Throat and nasopharyngeal virus cultures were taken from 67 ill residents; 33 cultures yielded rhinovirus, and no other respiratory virus was isolated. Geographic clustering of persons infected with rhinovirus was observed. Of those persons with rhinovirus infections, 100% had upper respiratory symptoms, 34% had gastrointestinal symptoms, 71% had systemic symptoms, 66% had lower respiratory symptoms (including productive cough), and 52% had new abnormalities on lung auscultation. The 17 persons with rhinovirus infection who had chronic obstructive pulmonary disease had more severe illnesses: Five (29%) required glucocorticoid or bronchodilator therapy for illness-associated bronchospasm; 2 required transfer out of the facility; 1 developed a radiographically documented infiltrate; and 1 died of respiratory failure. Conclusions: Rhinovirus may cause epidemic, clinically important respiratory illness in nursing home residents. A large proportion of residents may become ill, and infection may be severe in persons with underlying lung disease. C1 UNIV WISCONSIN,SCH MED,INST AGING,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. UNIV WISCONSIN,WISCONSIN STATE LAB HYG,MADISON,WI 53706. WISCONSIN VET HOME,KING,WI 54946. RI Gravenstein, Stefan/G-1681-2011 FU NIA NIH HHS [AG09632, AG00548, AG00213] NR 18 TC 68 Z9 68 U1 0 U2 1 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD OCT 15 PY 1995 VL 123 IS 8 BP 588 EP 593 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA RY828 UT WOS:A1995RY82800004 PM 7677299 ER PT J AU PRABHU, SD MURRAY, DR LELEUX, J FREEMAN, GL AF PRABHU, SD MURRAY, DR LELEUX, J FREEMAN, GL TI EFFECT OF RYANODINE ON LEFT-VENTRICULAR CONTRACTION AND RELAXATION IN CLOSED-CHEST DOGS - INSIGHTS INTO INTRACELLULAR CALCIUM HANDLING SO CIRCULATION LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 15 PY 1995 VL 92 IS 8 SU S BP 1214 EP 1214 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TB480 UT WOS:A1995TB48001208 ER PT J AU PRABHU, SD LELEUX, J MURRAY, DR FREEMAN, GL AF PRABHU, SD LELEUX, J MURRAY, DR FREEMAN, GL TI EFFECT OF RYANODINE ON THE LEFT-VENTRICULAR SYSTOLIC AND DIASTOLIC FORCE-FREQUENCY RELATION IN CLOSED-CHEST DOGS SO CIRCULATION LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY VET AFFAIRS HOSP,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 15 PY 1995 VL 92 IS 8 SU S BP 1215 EP 1215 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TB480 UT WOS:A1995TB48001209 ER PT J AU REUE, K COHEN, RD SLAVIN, BG AF REUE, K COHEN, RD SLAVIN, BG TI IMPAIRED FAT STORAGE AND ABERRANT EXPRESSION OF ADIPOCYTE DIFFERENTIATION MARKERS IN FATTY LIVER DYSTROPHY MUTANT MICE SO CIRCULATION LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. UNIV SO CALIF,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 15 PY 1995 VL 92 IS 8 SU S BP 1712 EP 1712 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TB480 UT WOS:A1995TB48001701 ER PT J AU SAGER, PT KOH, SW NGUYEN, T AF SAGER, PT KOH, SW NGUYEN, T TI BETA-ADRENERGIC STIMULATION ACCELERATES HUMAN ACTION-POTENTIAL ACCOMMODATION SO CIRCULATION LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. NR 0 TC 1 Z9 1 U1 1 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 15 PY 1995 VL 92 IS 8 SU S BP 3499 EP 3499 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TB480 UT WOS:A1995TB48003477 ER PT J AU SAGER, PT AF SAGER, PT TI FREQUENCY-DEPENDENT ELECTROPHYSIOLOGIC EFFECTS OF DOFETILIDE IN HUMANS SO CIRCULATION LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7322 J9 CIRCULATION JI Circulation PD OCT 15 PY 1995 VL 92 IS 8 SU S BP 3727 EP 3727 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA TB480 UT WOS:A1995TB48003703 ER PT J AU WALSH, JH PETERSON, WL AF WALSH, JH PETERSON, WL TI THE TREATMENT OF HELICOBACTER-PYLORI INFECTION IN THE MANAGEMENT OF PEPTIC-ULCER DISEASE SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Review ID RANDOMIZED CONTROLLED TRIAL; DOUBLE-BLIND TRIAL; RAPID UREASE TEST; DUODENAL-ULCER; CAMPYLOBACTER-PYLORI; TRIPLE THERAPY; METRONIDAZOLE RESISTANCE; BREATH TEST; ANTIMICROBIAL AGENTS; BISMUTH SUBCITRATE C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. UNIV TEXAS,SW MED SCH,DEPT INTERNAL MED,DALLAS,TX 75230. VET AFFAIRS MED CTR,MED SERV,DALLAS,TX. RP WALSH, JH (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,CTR GASTROENTER BIOL,LOS ANGELES,CA 90073, USA. FU NIDDK NIH HHS [DK 41301, DK 17294] NR 94 TC 361 Z9 368 U1 0 U2 6 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD OCT 12 PY 1995 VL 333 IS 15 BP 984 EP 991 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA RY586 UT WOS:A1995RY58600008 PM 7666920 ER PT J AU BROOKER, DR KOZAK, CA KLEYMAN, TR AF BROOKER, DR KOZAK, CA KLEYMAN, TR TI EPITHELIAL SODIUM-CHANNEL GENES SCNN1B AND SCNN1G ARE CLOSELY LINKED ON DISTAL MOUSE CHROMOSOME-7 SO GENOMICS LA English DT Note ID SKELETAL-MUSCLE; ALPHA-SUBUNIT; ISOFORMS; BRAIN AB The chromosomal localizations of Scnn1b and Scnn1g, genes corresponding to the beta- and gamma-subunits, respectively, of an epithelial non-voltage-gated amiloride-sensitive sodium channel, were determined by analyses of two sets of multilocus crosses using probes generated by polymerase chain reaction and a mouse kidney cortical collecting tubule cell line (M1). Scnn1b and Scnn1g were determined to be closely linked on distal mouse chromosome 7, showing no recombination with Zp2, whereas the gene for the alpha-subunit, Scnn1a, was confirmed to map to distal mouse chromosome 6. (C) 1995 Academic Press, Inc. C1 UNIV PENN,DEPT MED,DIV RENAL,PHILADELPHIA,PA 19104. UNIV PENN,DEPT PHYSIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. NIAID,BETHESDA,MD 20892. FU NIDDK NIH HHS [DK07006] NR 26 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD OCT 10 PY 1995 VL 29 IS 3 BP 784 EP 786 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA TA966 UT WOS:A1995TA96600033 PM 8575777 ER PT J AU PAYAMI, H MONTEE, K ZAREPARSI, S KAVE, J BIRD, T YU, C WIJSMAN, E HESTON, L LITT, M SCHELLENBERG, G AF PAYAMI, H MONTEE, K ZAREPARSI, S KAVE, J BIRD, T YU, C WIJSMAN, E HESTON, L LITT, M SCHELLENBERG, G TI FAMILIAL ALZHEIMER-DISEASE IN WOMEN SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 OREGON HLTH SCI UNIV,PORTLAND,OR. PORTLAND VET AFFAIRS MED CTR,PORTLAND,OR. VET AFFAIRS MED CTR,SEATTLE,WA. UNIV WASHINGTON,SEATTLE,WA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 970 EP 970 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68700969 ER PT J AU LIU, AWY DELGADOESCUETA, AV GEE, MN ZHAO, HZ SPELLMAN, JM MEDINA, MT ALONSO, ME CORDOVA, S DONNADIEU, FR PECK, R SPARKES, RS AF LIU, AWY DELGADOESCUETA, AV GEE, MN ZHAO, HZ SPELLMAN, JM MEDINA, MT ALONSO, ME CORDOVA, S DONNADIEU, FR PECK, R SPARKES, RS TI JUVENILE MYOCLONIC EPILEPSY IN CHROMOSOME 6P12-11 - RECOMBINATIONS NARROW INTO 6CM REGION AND LOCUS HETEROGENEITY SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,COMPREHENS EPILEPSY PROGRAM,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,W LOS ANGELES DVA MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. NATL INST NEUROL & NEUROSURG,MEXICO CITY,DF,MEXICO. NATL AUTONOMOUS UNIV TEGUCIGALPA,TEGUCIGALPA,HONDURAS. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 1131 EP 1131 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68701132 ER PT J AU SARRATOSA, JM DELGADOESCUETA, AV POSADA, I SHIH, S DRURY, I BERCIANO, J ZABALA, JA ANTUNEZ, MC SPARKES, RS AF SARRATOSA, JM DELGADOESCUETA, AV POSADA, I SHIH, S DRURY, I BERCIANO, J ZABALA, JA ANTUNEZ, MC SPARKES, RS TI A GENE FOR PROGRESSIVE MYOCLONUS EPILEPSY OF THE LAFORA TYPE MAPS TO CHROMOSOME 6Q SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,CALIF COMPREHENS EPILEPSY PROGRAM,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. HOSP DOCE OCTUBRE,DEPT NEUROL,MADRID,SPAIN. UNIV MICHIGAN HOSP,DEPT NEUROL,ANN ARBOR,MI 48109. HOSP MARQUES VALDECILLA,DEPT NEUROL,SANTANDER,SPAIN. CLIN PUERTA HIERRO,NEUROL SERV,MADRID,SPAIN. HOSP UNIV VIRGEN ARRIXACA,NEUROL SERV,MURCIA,SPAIN. UNIV CALIF LOS ANGELES,DEPT MED,DIV MED GENET,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 1167 EP 1167 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68701168 ER PT J AU SINGH, I KREMSER, K GHOSH, B SINGH, AK PAI, GS AF SINGH, I KREMSER, K GHOSH, B SINGH, AK PAI, GS TI ABNORMALITY IN TRANSLATIONAL REGULATION OF CATALASE IN DISORDERS OF PEROXISOMAL BIOGENESIS SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT PEDIAT,CHARLESTON,SC 29425. RALPH H JOHNSON VET AFFAIRS MED CTR,DEPT PATHOL,CHARLESTON,SC. NR 1 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 1454 EP 1454 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68701455 ER PT J AU SAINZ, J SERRATOSA, JM SHIH, S DELGADOESCUETA, AV AF SAINZ, J SERRATOSA, JM SHIH, S DELGADOESCUETA, AV TI CONSTRUCTION OF A 7 CM YAC CONTIG WITHIN THE REGION OF THE JUVENILE MYOCLONIC EPILEPSY GENE ON CHROMOSOME 6P12-P11 SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,CALIF COMPREHENS EPILEPSY PROGRAM,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 1563 EP 1563 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68701563 ER PT J AU GULLEY, ML PULITZER, DR EAGAN, PA CHO, CG SCHNEIDER, BG AF GULLEY, ML PULITZER, DR EAGAN, PA CHO, CG SCHNEIDER, BG TI EPSTEIN-BARR-VIRUS IS AN EARLY EVENT IN GASTRIC CARCINOGENESIS AND IS INDEPENDENT OF BCL2 EXPRESSION AND P53 ACCUMULATION SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD OCT PY 1995 VL 57 IS 4 SU S BP 1749 EP 1749 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA RW687 UT WOS:A1995RW68701748 ER PT J AU MYERS, GA HARMS, BA STARLING, JR AF MYERS, GA HARMS, BA STARLING, JR TI MANAGEMENT OF PARAESOPHAGEAL HERNIA WITH A SELECTIVE APPROACH TO ANTIREFLUX SURGERY SO AMERICAN JOURNAL OF SURGERY LA English DT Article ID GASTROESOPHAGEAL REFLUX; INTRATHORACIC STOMACH; HIATAL-HERNIA AB BACKGROUND: The role of an antireflux procedure in the management of paraesophageal hernia is controversial. To address this issue, we reviewed our experience with selective use of antireflux procedures in patients with pure paraesophageal hernia (type II; n = 26) and those with a partial sliding component (type III; n = 11). PATIENTS AND METHODS: Surgical repair was performed on diagnosis in all 37 patients. Competency of the lower esophageal sphincter was evaluated on the basis of reflux symptoms, and objectively, with endoscopy in 21 patients and 24-hour esophageal pH studies in 17 patients. Repair included an antireflux procedure in 11 patients, as indicated by reflux disease. RESULTS: Preoperatively, 80% of both type II and type III patients reported obstructive symptoms. Reflux symptoms were present in 27% of patients-19% of type II and 45% of type III patients. Endoscopy revealed esophagitis in 5 cases, and 24-hour pH studies indicated significant reflux in 3 of 17 patients. There were no operative deaths and 1 recurrence. Symptoms improved in 92% of patients after surgery. Medically manageable reflux was identified in 2 patients. CONCLUSIONS: Frequent obstructive symptoms and the potential for gastric volvulus indicate elective repair of paraesophageal hernia on diagnosis. Significant gastroesophageal reflux is less common, especially in type II patients, and excellent symptomatic results are obtained with selective application of an antireflux procedure. C1 UNIV WISCONSIN,DEPT SURG,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 14 TC 42 Z9 44 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD OCT PY 1995 VL 170 IS 4 BP 375 EP 380 DI 10.1016/S0002-9610(99)80307-9 PG 6 WC Surgery SC Surgery GA RW931 UT WOS:A1995RW93100013 PM 7573732 ER PT J AU CONNELLY, TJ AHERN, C CORONADO, R AF CONNELLY, TJ AHERN, C CORONADO, R TI KETAMINE, AT CLINICAL CONCENTRATIONS, DOES NOT ALTER THE FUNCTION OF CARDIAC SARCOPLASMIC-RETICULUM CALCIUM-RELEASE CHANNELS SO ANESTHESIA AND ANALGESIA LA English DT Article ID VENTRICULAR MYOCARDIUM; MUSCLE; MECHANISM; MEMBRANE; BINDING; RAT AB In the absence of sympathetically mediated stimulation, ketamine depresses myocardial contractility. This results from a decrease in the availability of intracellular Ca2+ for excitation-contraction coupling. Although sites of action other than the Ca2+ release channel of sarcoplasmic reticulum have been implicated, ketamine-induced alterations in Ca2+ efflux from the sarcoplasmic reticulum remain contentious. The purpose of the present study was to identify interactions of ketamine with the calcium release channel using sarcoplasmic reticulum enriched vesicles from porcine left ventricle. Ketamine did not alter [H-3]ryanodine binding at concentrations of 1 mM or less, while binding was almost completely inhibited at 10 mM. Gating and conductance of SR Ca2+ channels studied in planar bilayers was not altered by clinical concentrations of ketamine over the range of physiologic cytoplasmic free Ca2+ concentrations. Channel inactivation was observed at 10 mM ketamine, well in excess of clinical concentrations. These findings indicate that clinical concentrations of ketamine do not alter the function of the Ca2+ release channel. Alterations in intracellular Ca2+ homeostasis that result in depression of myocardial contractility must therefore result from effects at other sites along the excitation-contraction coupling pathway. C1 UNIV WISCONSIN,DEPT ANESTHESIOL,MADISON,WI. UNIV WISCONSIN,DEPT PHYSIOL,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 23 TC 5 Z9 6 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0003-2999 J9 ANESTH ANALG JI Anesth. Analg. PD OCT PY 1995 VL 81 IS 4 BP 849 EP 854 DI 10.1097/00000539-199510000-00033 PG 6 WC Anesthesiology SC Anesthesiology GA RX477 UT WOS:A1995RX47700033 PM 7574022 ER PT J AU GRAYBILL, JR NAJVAR, LK HOLMBERG, JD CORREA, A LUTHER, MF AF GRAYBILL, JR NAJVAR, LK HOLMBERG, JD CORREA, A LUTHER, MF TI FLUCONAZOLE TREATMENT OF CANDIDA-ALBICANS INFECTION IN MICE - DOES IN-VITRO SUSCEPTIBILITY PREDICT IN-VIVO RESPONSE SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; FUNGAL-INFECTIONS; ORAL CANDIDIASIS; TRIAL; EPIDEMIOLOGY; RESISTANCE; MORTALITY; GLABRATA; FUNGEMIA AB A series of fluconazole-susceptible and-fluconazole resistant Candida albicans fungal isolates mere used to infect mice intravenously. Mice were treated with varying doses of fluconazole beginning one day after infection. For all of the 6 fluconazole-susceptible isolates, fluconazole was highly effective at <0.25 mg/kg of body weight twice daily. By contrast, fluconazole was less effective in at least 6 of 10 fluconazole-resistant isolates and was ineffective at greater than or equal to 40 mg/kg twice daily in 4 fluconazole-resistant isolates. Although the correlation is not precise, in vitro susceptibility testing of C. albicans can predict in vivo response to fluconazole. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. RP GRAYBILL, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,DIV INFECT DIS 111F,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 24 TC 19 Z9 22 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD OCT PY 1995 VL 39 IS 10 BP 2197 EP 2200 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RX469 UT WOS:A1995RX46900006 PM 8619566 ER PT J AU ATKINSON, BA BOCANEGRA, R GRAYBILL, JR AF ATKINSON, BA BOCANEGRA, R GRAYBILL, JR TI TREATMENT OF MYCOBACTERIUM-HAEMOPHILUM INFECTION IN A MURINE MODEL WITH CLARITHROMYCIN, RIFABUTIN, AND CIPROFLOXACIN SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID HEMOPHILUM; AIDS AB An animal model of disseminated Mycobacterium haemophilum infection was utilized to compare treatment with azithromycin, ciprofloxacin, rifabutin, and the combination of clarithromycin with rifabutin. Following subcutaneous challenge with M. haemophilum, local and disseminated infection occurred only in immunosuppressed mice. For disseminated infection, ciprofloxacin was relatively ineffective therapy. Clarithromycin and rifabutin alone significantly reduced the tissue burden in the spleen after 4 weeks of therapy. Combination therapy with rifabutin and clarithromycin was superior to 4 weeks of treatment with the individual agents. When immunosuppressed mice were treated for 20 weeks with the combination of rifabutin and clarithromycin, the tissue burden remained reduced in the spleen at 1 month following the completion of therapy. Combined rifabutin and clarithromycin provide effective treatment for M. haemophilum in this model. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 21 TC 12 Z9 12 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD OCT PY 1995 VL 39 IS 10 BP 2316 EP 2319 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RX469 UT WOS:A1995RX46900028 PM 8619588 ER PT J AU NANAGARA, R LI, F BEUTLER, A HUDSON, A SCHUMACHER, HR AF NANAGARA, R LI, F BEUTLER, A HUDSON, A SCHUMACHER, HR TI ALTERATION OF CHLAMYDIA-TRACHOMATIS BIOLOGIC BEHAVIOR IN SYNOVIAL MEMBRANES - SUPPRESSION OF SURFACE-ANTIGEN PRODUCTION IN REACTIVE ARTHRITIS AND REITERS-SYNDROME SO ARTHRITIS AND RHEUMATISM LA English DT Article ID HUMAN POLYMORPHONUCLEAR LEUKOCYTES; MONOCLONAL-ANTIBODIES; ELECTRON-MICROSCOPY; HUMAN-MONOCYTES; RIBOSOMAL-RNA; PROTEIN; INFECTION; LIPOPOLYSACCHARIDE; MACROPHAGES; INTERFERON AB Objective. To investigate the biologic state of Chlamydia and its surface antigen expression in the synovial membranes of patients with Chlamydia-associated reactive arthritis/Reiter's syndrome (ReA/RS). Methods, Expression of chlamydial lipopolysaccharide (LPS), major outer membrane protein (MOMP), and elementary body (EB) antigens was studied by gold labeling immunoelectron microscopy on 6 synovial membrane and 2 synovial fluid (SF) pellet samples from 6 patients with Chlamydia-associated arthritis. The study findings were compared with 24-hour cultures of HeLa cells infected with Chlamydia trachomatis EB, Results, Persistent C trachomatis infection was found in all 6 synovial membrane samples from patients who had either early or chronic arthritis, The infection persisted despite antibiotic treatment, including a 1-month course of doxycycline therapy. Most persistent organisms were atypical reticulate bodies (RBs) found in both fibroblasts and macrophages. Specific, but weak, immunogold staining for all 3 antibodies was found on both intracellular RBs and extracellular EBs, In the SF samples, Chlamydia surface antigens were detected only in phagosomes containing degraded electron-dense materials. Conclusions. The synovial membrane biopsies conducted in this study of Chlamydia-associated ReA/RS revealed atypical RBs with diminished MOMP and LPS expression. Such altered organisms may escape immune surveillance and contribute to disease chronicity; moreover, these organisms may be difficult to detect and treat in some ReA/RS patients. C1 VET AFFAIRS MED CTR,CTR RHEUMATOL IMMUNOL,PHILADELPHIA,PA 19104. KHON KAEN UNIV,KHON KAEN,THAILAND. UNIV PENN,SCH MED,PHILADELPHIA,PA 19104. MED COLL PENN,PHILADELPHIA,PA 19129. FU NEI NIH HHS [EY-03324]; NIAMS NIH HHS [AR-42541] NR 45 TC 104 Z9 107 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD OCT PY 1995 VL 38 IS 10 BP 1410 EP 1417 DI 10.1002/art.1780381008 PG 8 WC Rheumatology SC Rheumatology GA TA574 UT WOS:A1995TA57400007 PM 7575691 ER PT J AU KOBAYASHI, M LAVER, JH KATO, T MIYAZAKI, H OGAWA, M AF KOBAYASHI, M LAVER, JH KATO, T MIYAZAKI, H OGAWA, M TI RECOMBINANT HUMAN THROMBOPOIETIN (MPL LIGAND) ENHANCES PROLIFERATION OF ERYTHROID PROGENITORS SO BLOOD LA English DT Note ID COLONY-STIMULATING FACTOR; SERUM-FREE CULTURE; BURST-PROMOTING ACTIVITY; HUMAN GM-CSF; HEMATOPOIETIC PROGENITORS; HUMAN-ERYTHROPOIETIN; GROWTH; CELLS; DIFFERENTIATION; INTERLEUKIN-3 AB We have studied the effects of recombinant human thrombopoietin (TPO, Mpl ligand) on human hematopoiesis in vitro. TPO alone did not support erythroid burst formation but, in the presence of erythropoietin, it enhanced erythroid burst formation from CD34(+) bone marrow and cord blood cells. The burst-promoting activity (BPA) was stronger under 5% serum than 30% serum conditions. The direct nature of BPA effects was documented by replating studies of early erythroid bursts. The BPA of TPO was less than that of interleukin-3 but was comparable with that of granulocyte/macrophage colony-stimulating factor and steel factor. The soluble form of Mpl receptor inhibited burst enhancing effects of TPO, suggesting that the BPA affects of TPO are mediated through the Mpl receptor. These results further delineate the physiologic roles of TPO and may aid in the determination of the clinical usages of TPO. (C) 1995 by The American Society of Hematology. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC 29401. MED UNIV S CAROLINA,DEPT PEDIAT & MED,CHARLESTON,SC 29425. KIRIN BREWERY CO LTD,PHARMACEUT RES LAB,MAEBASHI,GUMMA,JAPAN. FU NIDDK NIH HHS [DK32294] NR 39 TC 146 Z9 147 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD OCT 1 PY 1995 VL 86 IS 7 BP 2494 EP 2499 PG 6 WC Hematology SC Hematology GA RW497 UT WOS:A1995RW49700005 PM 7545458 ER PT J AU TELLES, C KARNO, M MINTZ, J PAZ, G ARIAS, M TUCKER, D LOPEZ, S AF TELLES, C KARNO, M MINTZ, J PAZ, G ARIAS, M TUCKER, D LOPEZ, S TI IMMIGRANT FAMILIES COPING WITH SCHIZOPHRENIA - BEHAVIORAL FAMILY INTERVENTION V CASE-MANAGEMENT WITH A LOW-INCOME SPANISH-SPEAKING POPULATION SO BRITISH JOURNAL OF PSYCHIATRY LA English DT Article ID EXPRESSED EMOTION; CONTROLLED TRIAL; RELAPSE AB Background. This investigation compared the effectiveness and cross-cultural applicability of behavioural family management (BFM) and standard case management in preventing exacerbation of symptoms and relapse in schizophrenia. Method. Forty low-income Spanish-speaking people with a diagnosis of schizophrenia were randomly assigned to receive standard case management or behavioural family management after stabilisation with neuroleptic medication. Results. Survival analyses indicated that among the less acculturated patients BFM was significantly related to greater risk of exacerbation of symptoms. Among the more acculturated patients, risk of exacerbation could be predicted by medication compliance but not by type of intervention. In analyses of symptom severity and functional status at 1-year follow-up, the level of patient acculturation was found to be significantly related to various measures of treatment outcome. Conclusion. Sociocultural factors affect responses to different types of intervention. The results did not support earlier findings of a beneficial effect of BFM when applied to a socioculturally diverse population. C1 W LOS ANGELES VET ASSOC MED CTR,LOS ANGELES,CA. SANTA MONICA W MENTAL HLTH SERV,SANTA MONICA,CA. RP TELLES, C (reprint author), UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,300 UCLA MED PLAZA,SUITE 2236,LOS ANGELES,CA 90024, USA. RI Mintz, Jim/N-7385-2014 OI Mintz, Jim/0000-0002-8299-5851 FU NIMH NIH HHS [MH 30911] NR 22 TC 89 Z9 89 U1 2 U2 5 PU ROYAL COLLEGE OF PSYCHIATRISTS PI LONDON PA BRITISH JOURNAL OF PSYCHIATRY 17 BELGRAVE SQUARE, LONDON, ENGLAND SW1X 8PG SN 0007-1250 J9 BRIT J PSYCHIAT JI Br. J. Psychiatry PD OCT PY 1995 VL 167 BP 473 EP 479 DI 10.1192/bjp.167.4.473 PG 7 WC Psychiatry SC Psychiatry GA TA964 UT WOS:A1995TA96400010 PM 8829715 ER PT J AU CAO, J HONG, CH ROSEN, L VESCIO, RA SMULSON, M LICHTENSTEIN, AK BERENSON, JR AF CAO, J HONG, CH ROSEN, L VESCIO, RA SMULSON, M LICHTENSTEIN, AK BERENSON, JR TI DELETION OF GENETIC MATERIAL FROM A POLY(ADP-RIBOSE) POLYMERASE-LIKE GENE ON CHROMOSOME-13 OCCURS FREQUENTLY IN PATIENTS WITH MONOCLONAL GAMMOPATHIES SO CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION LA English DT Article ID MULTIPLE-MYELOMA; ADP-RIBOSYLATION; UNITED-STATES; DNA; CANCER; CELLS; CARCINOGENESIS; EXPRESSION; DEPLETION; LEUKEMIA AB Recently, by using a probe far the nuclear DNA repair enzyme poly(ADP-ribose) polymerase gene, a pseudogene was found on the long arm of chromosome 13, RFLP analysis demonstrates the presence of a common ''A'' allele and a rare (''B'' allele, which has a deletion of approximately 200 bp, This deletion occurs more frequently in blacks than in whites in the United States, In two B-cell malignancies, Burkitt's and follicular lymphomas, there is a marked increased frequency of the expression of the B allele. Thus, we have analyzed the frequency of this allele in another B-cell malignancy, multiple myeloma (MM), which is also more frequently observed in blacks, We studied 97 patients with MM (41 black and 56 white patients) and 30 patients with the related disorder monoclonal gammopathy of undetermined significance (MGUS; 13 black and 17 white patients), The results demonstrate that the overall frequency of B allele expression (37%) is higher than in a noncancer control population (23%; P < 0.01), This difference is mainly due to the much higher frequency of B expression in black patients (62 versus 35% in black controls; P < 0.01), whereas there is no significant difference in white patients (18 versus 14% in white controls), Overall, B allelic frequency is similar in patients with MM and MGUS. Matched germline and tumor DNA show identical patterns of expression of these alleles, These results suggest germline B allelic expression predisposes one to MM and MGUS. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,DIV HEMATOL ONCOL,LOS ANGELES,CA 90073. JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90024. GEORGETOWN UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,WASHINGTON,DC 20007. NR 30 TC 14 Z9 15 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 1055-9965 J9 CANCER EPIDEM BIOMAR JI Cancer Epidemiol. Biomarkers Prev. PD OCT-NOV PY 1995 VL 4 IS 7 BP 759 EP 763 PG 5 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA RZ110 UT WOS:A1995RZ11000010 PM 8672993 ER PT J AU LI, JJ LI, SA OBERLEY, TD PARSONS, JA AF LI, JJ LI, SA OBERLEY, TD PARSONS, JA TI CARCINOGENIC ACTIVITIES OF VARIOUS STEROIDAL AND NONSTEROIDAL ESTROGENS IN THE HAMSTER-KIDNEY - RELATION TO HORMONAL ACTIVITY AND CELL-PROLIFERATION SO CANCER RESEARCH LA English DT Article ID SYRIAN-HAMSTER; ANTI-ESTROGENS; GOLDEN-HAMSTER; TUBULAR CELLS; METABOLISM; RECEPTOR; ADENOCARCINOMA; TUMORIGENESIS; INDUCTION; TISSUES AB The therapeutic use of estrogens has been associated with an increased risk of same of the most predominant, as well as less prevalent, cancers in women, The estrogen-induced renal tumor is one of the primary animal models to evaluate the carcinogenic properties of estrogens. Correlations were made with various estrogens by using parameters of estrogenicity end points such as competitive binding, progesterone receptor induction, and alterations in prolactin levels; in vitro renal proximal cell proliferation; and in vivo estrogen-induced carcinogenicity. The most potent estrogens were Moxestrol (MOX), diethylstilbestrol (DES), and 17 beta-estradiol, followed by indenestrol B, 16 alpha-hydroxyestrone, and 11 beta-methoxyestradiol with moderate estrogenic activities, whereas 11 beta-methytestradiol, 17 alpha-estradiol, indanestrol, and deoxoestrone were all relatively weaker. As expected, hydrolyzed Premarin (unconjugated estrogens) was strongly estrogenic Of the estrogens tested, MOX was the most potent carcinogenic estrogen in the hamster kidney. Both 16 alpha-hydroxyestrone and 11 beta-methoxyestradiol induced intermediate tumor incidences with distinctly lower frequencies of renal tumor foci compared to the most potent carcinogenic estrogens. However, hamsters treated for 9.0 months with 11 beta-methylestradiol, 17 alpha-estradiol, deoxoestrone, and indanestrol exhibited no tumors. In contrast, treatment with estrone, equilin plus d-equilenin, and hydrolyzed Premarin for the same time period resulted in 100% renal tumor incidences and numerous tumor foci. Cell proliferation studies of cultured hamster kidney proximal tubule cells were carried out at varying estrogen concentrations (0.01-100 nM). Exposure to MOX resulted in consistently high renal cell proliferative response over a concentration range of 0.1-10 nM. Strongly carcinogenic estrogens such as estrone had a maximal renal cell proliferation response (2.4-fold above untreated control levels) between 0.1 and 10 nM, DES and 17 beta-estradiol responded at 1.0 nM, and 4-hydroxyestradiol responded at 10 nM. Interestingly, exposure to ethinylestradiol, a potent estrogen, at similar or higher doses as those used for DES and 17 beta-estradiol, yielded only a 10% renal tumor incidence and induced only a 1.7-fold increase in proximal tubule cell proliferation. In contrast, 17 alpha-estradiol, deoxoestrone, indanestrol, and 11 beta-methylestradiol, all weakly estrogenic and noncarcinogenic agents, had relatively little effect on tubule cell proliferation. The hydrolyzed Premarin exhibited a maximal 2.0-fold cell proliferative response at 10 nM. The present results provide clear evidence that, in the hamster kidney, the degree of carcinogenicity of a given estrogen correlates with its ability to induce proximal tubule cell proliferation in vitro. Therefore, the ability of estrogen to enhance tubule cell proliferation is a more accurate indicator of its carcinogenicity in this system than either the estrogen-responsive end points used or the amount of catechol metabolites generated in this tissue as reported earlier. C1 UNIV KANSAS, MED CTR, DEPT PHARMACOL TOXICOL & THERAPEUT, KANSAS CITY, KS 66160 USA. UNIV KANSAS, MED CTR, DEPT PREVENT MED, KANSAS CITY, KS 66160 USA. UNIV WISCONSIN, SCH MED, DEPT PATHOL, MADISON, WI 53706 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, PATHOL SERV, MADISON, WI 53706 USA. UNIV MINNESOTA, SCH MED, DEPT ANAT, MINNEAPOLIS, MN 55417 USA. RP LI, JJ (reprint author), UNIV KANSAS, MED CTR, CANC CTR, DIV ETIOL & PREVENT HORMONAL CANC, HORMONAL CARCINOGENESIS LAB, KANSAS CITY, KS 66160 USA. FU NCI NIH HHS [CA58030, CA22008] NR 48 TC 107 Z9 107 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD OCT 1 PY 1995 VL 55 IS 19 BP 4347 EP 4351 PG 5 WC Oncology SC Oncology GA RX114 UT WOS:A1995RX11400028 PM 7671246 ER PT J AU Mody, FV Singh, BN AF Mody, FV Singh, BN TI Silent ischemia in the elderly - Overview SO CARDIOLOGY IN THE ELDERLY LA English DT Editorial Material ID MYOCARDIAL-ISCHEMIA; UNSTABLE ANGINA C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. RP Mody, FV (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CARDIOL SECT,DIV CARDIOL,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 10 TC 0 Z9 0 U1 0 U2 0 PU CURRENT SCIENCE PI PHILADELPHIA PA 400 MARKET STREET,SUITE 750 ATTN:SARAH WHEALEN/SUB MGR, PHILADELPHIA, PA 19106 SN 1058-3661 J9 CARDIOL ELDER JI Cardiol. Elder. PD OCT PY 1995 VL 3 IS 5 BP 321 EP 325 PG 5 WC Cardiac & Cardiovascular Systems; Geriatrics & Gerontology SC Cardiovascular System & Cardiology; Geriatrics & Gerontology GA TR206 UT WOS:A1995TR20600001 ER PT J AU PAHLAVANI, MA HARRIS, MD RICHARDSON, A AF PAHLAVANI, MA HARRIS, MD RICHARDSON, A TI THE AGE-RELATED DECLINE IN THE INDUCTION OF IL-2 TRANSCRIPTION IS CORRELATED TO CHANGES IN THE TRANSCRIPTION FACTOR NFAT SO CELLULAR IMMUNOLOGY LA English DT Article ID PERIPHERAL-BLOOD LYMPHOCYTES; HUMAN INTERLEUKIN-2 GENE; 5' FLANKING REGION; T-CELL ACTIVATION; CYCLOSPORINE-A; IMMUNE FUNCTION; RECEPTOR EXPRESSION; ANTIGEN RECEPTOR; NUCLEAR FACTOR; PROTEIN AB The effect of aging on the induction of IL-2 transcription by concanavalin A was studied in spleen lymphocytes isolated from young (4-6 months) and old (24 months) male Fischer 344 rats. The induction of IL-2 mRNA levels and IL-2 transcription were 40 to 50% lower for spleen lymphocytes isolated from old rats compared to spleen lymphocytes isolated from young rats. Because the transcription factor NFAT (nuclear factor activating IL-2 transcription) plays a major role in the regulation of IL-2 transcription, the DNA binding activity of NFAT in nuclear extracts of concanavalin A-stimulated lymphocytes was measured using a gel shift assay. The ability of nuclear extracts isolated from either total spleen lymphocytes or T cells to bind the NFAT oligonucleotide decreased 50 to 60% with age. Therefore, the age-related decrease in the expression of IL-2 appears to occur at the level of transcription and involves the transcription factor NFAT. (C) 1995 Academic Press, Inc. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. RP PAHLAVANI, MA (reprint author), UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284, USA. FU NIA NIH HHS [AG01548, AG00677] NR 54 TC 27 Z9 27 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0008-8749 J9 CELL IMMUNOL JI Cell. Immunol. PD OCT 1 PY 1995 VL 165 IS 1 BP 84 EP 91 DI 10.1006/cimm.1995.1190 PG 8 WC Cell Biology; Immunology SC Cell Biology; Immunology GA RX039 UT WOS:A1995RX03900012 PM 7671328 ER PT J AU SALVATI, EA LIEBERMAN, JR HUK, OL EVANS, BG AF SALVATI, EA LIEBERMAN, JR HUK, OL EVANS, BG TI COMPLICATIONS OF FEMORAL AND ACETABULAR MODULARITY SO CLINICAL ORTHOPAEDICS AND RELATED RESEARCH LA English DT Article; Proceedings Paper CT 23rd Open Scientific Meeting of the Hip-Society CY FEB, 1995 CL ORLANDO, FL SP Hip Soc, Assoc Arthrit Hip & Knee Surgeons ID PROSTHESES AB The versatility of modular total hip arthroplasties have rapidly extended their applications. However, these new interfaces can lead to complications that were not observed with monolithic components. These problems have been noted with modular femoral and acetabular components and have been associated with the generation of particulate debris. This article reviews the authors' clinical observations and histologic, biomechanic, and spectophotometric evaluations of modular total hip arthroplasties. New data comparing both synovial fluid metal levels in well-fixed and loose monolithic and modular prosthetic hip implants are presented. In modular total hip components synovial fluid cobalt levels correlated positively with patient weight and length of implantation. The generation of particulate debris in modular total hip components may induce periprosthetic osteolysis. Taper locks for femoral components and locking mechanisms for the polyethylene liner and metallic cup must be designed to avoid the production of particulate debris. C1 UNIV CALIF LOS ANGELES,MED CTR,SCH MED,CTR HLTH SCI,DEPT ORTHOPAED SURG,LOS ANGELES,CA 90095. HOSP SPECIAL SURG,HIP & KNEE SERV,NEW YORK,NY 10021. CORNELL UNIV,NEW YORK HOSP,COLL MED,NEW YORK,NY. W LOS ANGELES VET ADM HOSP,LOS ANGELES,CA. MCGILL UNIV,SIR MORTIMER B DAVIS JEWISH HOSP,DIV ORTHOPAED SURG,MONTREAL,PQ,CANADA. GEORGETOWN UNIV,DEPT ORTHOPAED SURG,WASHINGTON,DC. NR 16 TC 34 Z9 34 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0009-921X J9 CLIN ORTHOP RELAT R JI Clin. Orthop. Rel. Res. PD OCT PY 1995 IS 319 BP 85 EP 93 PG 9 WC Orthopedics; Surgery SC Orthopedics; Surgery GA TA785 UT WOS:A1995TA78500009 PM 7554653 ER PT J AU HISNANICK, JJ CODDINGTON, DA AF HISNANICK, JJ CODDINGTON, DA TI MEASURING HUMAN BETTERMENT THROUGH AVOIDABLE MORTALITY - A CASE FOR UNIVERSAL HEALTH-CARE IN THE USA SO HEALTH POLICY LA English DT Article DE AVOIDABLE MORTALITY; HEALTH CARE REFORM ID MEDICAL INTERVENTION; SOCIAL INEQUITIES; SERVICES; QUALITY; TRENDS; SWEDEN AB The USA system of health care has begun a monumental change that will affect everyone, irrespective of their socioeconomic status, professional status or pre-existing health insurance status, Whatever type of plan is finally implemented through the legislative process, there will need to be a way to evaluate its success (or failure). One way to evaluate the plan's effectiveness is through its impact on human betterment as viewed by a reduction in 'avoidable mortality' for those most in need of health care; the poor and uninsured. For one USA minority population, universal health care has improved human betterment by reducing avoidable mortality, even in the face of a severe burden of poverty. C1 US DEPT VET AFFAIRS,NATL CTR VET ANAL & STAT 008C12,WASHINGTON,DC 20420. GEORGE WASHINGTON UNIV,CHILDRENS NATL MED CTR,DEPT GEN PEDIAT,WASHINGTON,DC 20001. RP HISNANICK, JJ (reprint author), US DEPT VET AFFAIRS,NATL CTR VET ANAL & STAT 008C12,810 VERMONT AVE NW,WASHINGTON,DC 20420, USA. NR 32 TC 6 Z9 6 U1 0 U2 2 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0168-8510 J9 HEALTH POLICY JI Health Policy PD OCT PY 1995 VL 34 IS 1 BP 9 EP 19 DI 10.1016/0168-8510(94)00715-Q PG 11 WC Health Care Sciences & Services; Health Policy & Services SC Health Care Sciences & Services GA TA314 UT WOS:A1995TA31400002 PM 10151967 ER PT J AU LEO, MA ALEYNIK, SI SIEGEL, JH KASMIN, F ALEYNIK, MK LIEBER, CS AF LEO, MA ALEYNIK, SI SIEGEL, JH KASMIN, F ALEYNIK, MK LIEBER, CS TI F-2-ISOPROSTANE EXCRETION IN HUMAN BILE OF PATIENTS WITH BILIARY-TRACT AND PANCREATIC DISORDERS SO HEPATOLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR,ALCOHOL RES CTR,LIVER DIS & NUTR SECT,NEW YORK,NY. BETH ISRAEL MED CTR,ALCOHOL RES CTR,LIVER DIS & NUTR SECT,NORTH DIV,NEW YORK,NY. MT SINAI SCH MED,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD OCT PY 1995 VL 22 IS 4 SU S BP 12 EP 12 PN 2 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RX690 UT WOS:A1995RX69000011 ER PT J AU LIEBER, CS LEO, MA ALEYNIK, SI ALEYNIK, MA DECARLI, LM AF LIEBER, CS LEO, MA ALEYNIK, SI ALEYNIK, MA DECARLI, LM TI POLYENYLPHOSPHATIDYLCHOLINE (PPC) DECREASES OXIDANT STRESS AND PROTECTS AGAINST ALCOHOL-INDUCED LIVER-INJURY IN THE BABOON SO HEPATOLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR,ALCOHOL RES CTR,LIVER DIS & NUTR SECT,NEW YORK,NY. MT SINAI SCH MED,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD OCT PY 1995 VL 22 IS 4 SU S BP 475 EP 475 PN 2 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RX690 UT WOS:A1995RX69000473 ER PT J AU ALEYNIK, SI LEO, MA ALEYNIK, MK LIEBER, CS AF ALEYNIK, SI LEO, MA ALEYNIK, MK LIEBER, CS TI INCREASED CIRCULATING F-2-ISOPROSTANES (F-2-IP) IN PATIENTS WITH SEVERE ALCOHOLIC LIVER-DISEASE SO HEPATOLOGY LA English DT Meeting Abstract C1 BRONX VET ADM MED CTR,ALCOHOL RES CTR,LIVER DIS & NUTR SECT,NEW YORK,NY. MT SINAI SCH MED,NEW YORK,NY. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD OCT PY 1995 VL 22 IS 4 SU S BP 554 EP 554 PN 2 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RX690 UT WOS:A1995RX69000554 ER PT J AU MITCHEN, J BLETZINGER, D RAGO, R WILDING, G AF MITCHEN, J BLETZINGER, D RAGO, R WILDING, G TI USE OF A DNA MICROFLUOROMETRIC ASSAY TO MEASURE PROLIFERATIVE RESPONSE OF MINK LUNG-CELLS TO PURIFIED TGF-BETA AND TO TGF-BETA ACTIVITY FOUND IN PROSTATE CELL-CONDITIONED MEDIUM SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Article DE TGF-BETA; MINK LUNG ASSAY; DNA MICROFLUORESCENT ASSAY; CONDITIONED MEDIUM; GROWTH FACTORS ID GROWTH FACTOR-BETA; EMBRYO FIBROBLASTS; EPITHELIAL-CELLS; CANCER; SEQUENCE; CULTURE; INVITRO; REGION; MTT AB The proliferative response of Mv1Lu cells to purified TGF beta(1), or TGF beta-like activity released by various cells into medium conditioned over a 24-h period was quantitated by adapting a rapid DNA fluorometric assay. Acid activation of the conditioned medium allowed the amount of biologically latent versus active TGF beta to be quantitated. A neutralizing antibody specific for TGF beta(1, 1.2 and 2.0) completely blocked the growth inhibition observed treating Mv1Lu cells with either purified TGF beta(1) or medium containing secreted TGF beta-like activity conditioned by DU145 prostate cells. In contrast to other assays commonly used to measure TGF beta activity, the proliferative response is related directly to DNA content rather than as a reflection of enzymatic activity or incorporation of H-3-thymidine. The necessity for radioactive isotope usage has been eliminated, and the biological response can be quantitated over a period of days. C1 UNIV WISCONSIN,CTR CLIN CANC,DEPT HUMAN ONCOL,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. FU NCI NIH HHS [CA 50590, CA 09614] NR 27 TC 5 Z9 5 U1 0 U2 0 PU SOC IN VITRO BIOLOGY PI COLUMBIA PA 8815 CENTRE PARK DRIVE SUITE 210, COLUMBIA, MD 21045 SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD OCT PY 1995 VL 31 IS 9 BP 692 EP 697 PG 6 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA TB080 UT WOS:A1995TB08000009 PM 8564080 ER PT J AU HUGHES, DE WRIGHT, KR UY, HL SASAKI, A YONEDA, T ROODMAN, GD MUNDY, GR BOYCE, BF AF HUGHES, DE WRIGHT, KR UY, HL SASAKI, A YONEDA, T ROODMAN, GD MUNDY, GR BOYCE, BF TI BISPHOSPHONATES PROMOTE APOPTOSIS IN MURINE OSTEOCLASTS IN-VITRO AND IN-VIVO SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article ID TRANSFORMING GROWTH FACTOR-BETA-1; BONE-MARROW CELLS; DNA FRAGMENTATION; PAGETS-DISEASE; NUDE-MICE; RESORPTION; DEATH; DIPHOSPHONATES; MACROPHAGES; INVITRO AB Bisphosphonates inhibit bone resorption and are therapeutically effective in diseases of increased bone turnover, such as Pagers disease and hypercalcemia of malignancy. The mechanisms by which they act remain unclear, Proposed mechanisms include inhibition of osteoclast formation from precursors and inhibitory or toxic effects on mature osteoclasts. We have developed a new in vitro model to study osteoclast survival and in this paper present in vitro and in vivo evidence that may explain both the observed reduction in osteoclast numbers and in bone resorption by mature osteoclasts, namely that bisphosphonates induce programmed cell death (apoptosis). Three bisphosphonates (risedronate, pamidronate, and clodronate) caused a 4- to 24-fold increase in the proportion of osteoclasts showing the characteristic morphology of apoptosis in vitro, This observation was confirmed in vivo in normal mice, in mice with increased bone resorption, and in nude mice with osteolytic cancer metastases, with similar-fold increases to those observed in vitro. Of the three compounds, risedronate, the most potent inhibitor of bone resorption in vivo, was the strongest inducer of osteoclast apoptosis in vitro. Osteoclast apoptosis may therefore be a major mechanism whereby bisphosphonates reduce osteoclast numbers and activity, and induction of apoptosis could be a therapeutic goal for new antiosteoclast drugs. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. FU NCI NIH HHS [CA 40035]; NIAMS NIH HHS [AR-39529]; NIDCR NIH HHS [DE 08569] NR 54 TC 679 Z9 710 U1 2 U2 12 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD OCT PY 1995 VL 10 IS 10 BP 1478 EP 1487 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RX780 UT WOS:A1995RX78000007 PM 8686503 ER PT J AU SINGH, BN KOWEY, PR AF SINGH, BN KOWEY, PR TI CHANGING PERSPECTIVES IN CARDIAC-ARRHYTHMIA CONTROL - AGENTS, TECHNIQUES, GOALS, AND OUTCOMES - INTRODUCTION SO JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY LA English DT Editorial Material RP SINGH, BN (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CARDIOL SECT 691111E,WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FUTURA PUBL CO PI ARMONK PA 135 BEDFORD RD, PO BOX 418, ARMONK, NY 10504-0418 SN 1045-3873 J9 J CARDIOVASC ELECTR JI J. Cardiovasc. Electrophysiol. PD OCT PY 1995 VL 6 IS 10 BP 865 EP 867 DI 10.1111/j.1540-8167.1995.tb00362.x PN 2 PG 3 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA TB521 UT WOS:A1995TB52100001 ER PT J AU SINGH, BN AF SINGH, BN TI EXPANDING INDICATIONS FOR THE USE OF CLASS-III AGENTS IN PATIENTS AT HIGH-RISK FOR SUDDEN-DEATH SO JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY LA English DT Article DE AMIODARONE; SOTALOL; D-SOTALOL; ARRHYTHMIA SUPPRESSION; CLINICAL TRIALS ID COMPLEX VENTRICULAR ARRHYTHMIAS; MYOCARDIAL-INFARCTION; ANTIARRHYTHMIC DRUGS; DILATED CARDIOMYOPATHY; AMIODARONE; MORTALITY; METAANALYSIS; QUINIDINE; THERAPY; TRIAL AB The evidence that antiarrhythmic compounds that act by slowing conduction velocity increase mortality in patients with cardiac disease is now compelling. Emphasis is now shifting to agents that act by lengthening repolarization and have additional antiadrenergic properties. There is preliminary evidence that pure Class III agents devoid of antisympathetic activity may also increase rather than decrease mortality in certain patients. Thus, in recent years, sotalol and amiodarone have emerged as the preferred agents for the control of most ventricular arrhythmias occurring in the setting of significant heart disease. Sotalol has not been widely studied in postinfarct patients; one trial indicated that the drug did reduce total mortality but the difference did not reach statistical significance. A number of studies with amiodarone in the postmyocardial infarction patients have revealed benefit, but these were from nonblinded studies. Two blinded, placebo-controlled studies are currently ongoing. A potential new indication of amiodarone is in patients with arrhythmias in heart failure in whom amiodarone markedly increased left ventricular ejection fraction, with a pronounced suppressant effect on premature ventricular complexes and nonsustained ventricular tachycardia and a trend for a decrease in mortality in patients with nonischemic cardiomyopathy. The most promising indication of amiodarone in low doses is in the maintenance of sinus rhythm in patients with atrial butter and fibrillation. For the present, amiodarone appears to be the-best prototype of a desirable complex antiarrhythmic compound, if its variegated side effect profile can be favorably modified from knowledge of structure-activity relationships. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. RP SINGH, BN (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, DIV CARDIOL, CARDIOL SECT 691111E, WILSHIRE & SAWTELLE BLVD, LOS ANGELES, CA 90073 USA. NR 47 TC 33 Z9 34 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1045-3873 EI 1540-8167 J9 J CARDIOVASC ELECTR JI J. Cardiovasc. Electrophysiol. PD OCT PY 1995 VL 6 IS 10 BP 887 EP 900 DI 10.1111/j.1540-8167.1995.tb00365.x PN 2 PG 14 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA TB521 UT WOS:A1995TB52100004 PM 8548110 ER PT J AU DALLAS, SL MIYAZONO, K SKERRY, TM MUNDY, GR BONEWALD, LF AF DALLAS, SL MIYAZONO, K SKERRY, TM MUNDY, GR BONEWALD, LF TI DUAL ROLE FOR THE LATENT TRANSFORMING GROWTH-FACTOR-BETA BINDING-PROTEIN IN STORAGE OF LATENT TGF-BETA IN THE EXTRACELLULAR-MATRIX AND AS A STRUCTURAL MATRIX PROTEIN SO JOURNAL OF CELL BIOLOGY LA English DT Article ID SMOOTH-MUSCLE CELLS; PLASMINOGEN-ACTIVATOR INHIBITOR; CONNECTIVE-TISSUE MICROFIBRILS; MOLECULAR-WEIGHT COMPLEX; MARFAN-SYNDROME; MESSENGER-RNA; HUMAN-PLATELETS; EXPRESSION; FACTOR-BETA-1; FIBRILLIN AB The role of the latent TGF-beta binding protein (LTBP) is unclear. In cultures of fetal rat calvarial cells, which form mineralized bonelike nodules, both LTBP and the TGF-beta 1 precursor localized to large fibrillar structures in the extracellular matrix. The appearance of these fibrillar structures preceded the appearance of type I collagen fibers. Plasmin treatment abolished the fibrillar staining pattern for LTBP and released a complex containing both LTBP and TGF-beta. Antibodies and antisense oligonucleotides against LTBP inhibited the formation of mineralized bonelike nodules in long-term fetal rat calvarial cultures. Immunohistochemistry of fetal and adult rat bone confirmed a fibrillar staining pattern for LTBP in vivo. These findings, together with the known homology of LTBP to the fibrillin family of proteins, suggest a novel function for LTBP, in addition to its role in matrix storage of latent TGF-beta, as a structural matrix protein that may play a role in bone formation. C1 LUDWIG INST CANC RES,S-75124 UPPSALA,SWEDEN. UNIV BRISTOL,DEPT ANAT,BRISTOL BS2 8EJ,AVON,ENGLAND. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT CELLULAR & STRUCT BIOL,SAN ANTONIO,TX 78284. DEPT VET AFFAIRS,AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP DALLAS, SL (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL & METAB,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 56 TC 194 Z9 200 U1 1 U2 5 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD OCT PY 1995 VL 131 IS 2 BP 539 EP 549 DI 10.1083/jcb.131.2.539 PG 11 WC Cell Biology SC Cell Biology GA RZ595 UT WOS:A1995RZ59500020 PM 7593177 ER PT J AU TO, WK FOTHERGILL, AW RINALDI, MG AF TO, WK FOTHERGILL, AW RINALDI, MG TI COMPARATIVE-EVALUATION OF MACRODILUTION AND ALAMAR COLORIMETRIC MICRODILUTION BROTH METHODS FOR ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEAST ISOLATES SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID FLUCONAZOLE; RESISTANCE AB A comparative evaluation of the macrodilution method and the Alamar colorimetric method for the susceptibility testing of amphotericin B, fluconazole, and flucytosine was conducted with 134 pathogenic yeasts, The clinical isolates included 28 Candida albicans, 17 Candida tropicalis, 15 Candida parapsilosis, 12 Candida krusei, 10 Candida lusitaniae, 9 Candida guilliermondii, 18 Torulopsis glabrata, and 25 Cryptococcus neoformans isolates. The macrodilution method was performed and interpreted according to the recommendations of the National Committee for Clinical Laboratory Standards (document M27-P), and the Alamar colorimetric method was performed according to the manufacturer's instructions. For the Alamar colorimetric method, MICs mere determined at 24 and 48 h of incubation for Candida species and T. glabrata and at 48 and 72 h of incubation for C. neoformans. The overall agreement within +/- 1 dilution for Candida species and T. glabrata against the three antifungal agents was generally good, with the values for amphotericin B, fluconazole, and flucytosine being 85.3, 77.9, and 86.2%, respectively, at the 24-h readings and 69.3, 65.2, and 97.2%, respectively, at the 48-h readings, Most disagreement was noted with fluconazole against C. tropicalis and T. glabrata. Our studies indicate that determination of MICs at 24 h by the Alamar colorimetric method is a valid alternate method for testing amphotericin B, fluconazole, and flucytosine against Candida species but not for testing fluconazole against C. tropicalis and T. glabrata. For flucytosine, much better agreement can be demonstrated against Candida species and T. glabrata at the 48-h readings by the Alamar method, Excellent agreement within +/- 1 dilution can also be observed for amphotericin B, fluconazole, and flucytosine (80, 96, and 96%, respectively) against C, neoformans when the MICs were determined at 72 h by the Alamar method. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP TO, WK (reprint author), PRINCESS MARGARET HOSP,DEPT PATHOL,2-10 PRINCESS MARGARET HOSP RD,KOWLOON,HONG KONG. NR 26 TC 49 Z9 50 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD OCT PY 1995 VL 33 IS 10 BP 2660 EP 2664 PG 5 WC Microbiology SC Microbiology GA RV565 UT WOS:A1995RV56500025 PM 8567901 ER PT J AU MAAS, JW MILLER, AL TEKELL, JL FUNDERBURG, L SILVA, JA TRUE, J VELLIGAN, D BERMAN, N BOWDEN, CL AF MAAS, JW MILLER, AL TEKELL, JL FUNDERBURG, L SILVA, JA TRUE, J VELLIGAN, D BERMAN, N BOWDEN, CL TI CLONIDINE PLUS HALOPERIDOL IN THE TREATMENT OF SCHIZOPHRENIA PSYCHOSIS SO JOURNAL OF CLINICAL PSYCHOPHARMACOLOGY LA English DT Note ID NOREPINEPHRINE; NORADRENALINE; DOPAMINE; PLASMA; DRUGS; CSF AB Because of the evidence for increased norepinephrine (NE) production in psychotic patients, we studied the effects of combining the az-adrenergic agonist clonidine with haloperidol for the treatment of schizophrenic psychosis. Twelve hospitalized schizophrenic patients were taken off their antipsychotic medication for 2 to 4 weeks before double-blind treatment with haloperidol (20 mg/day) combined with either clonidine or placebo. The group receiving clonidine was significantly more improved on the thought disorder subscale of the Brief Psychiatric Rating Scale (p = 0.02). The groups differed initially in the level of negative symptoms, but controlling for this difference statistically by analysis of covariance did not change the finding with regard to the superiority of combining clonidine with haloperidol. We conclude that larger treatment trials of combining haloperidol with clonidine are warranted. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,SAN ANTONIO,TX 78284. SAN ANTONIO STATE HOSP,CLIN RES UNIT,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. UNIV CALIF LOS ANGELES,HARBOR MED CTR,INST EDUC RES,TORRANCE,CA 90509. NR 22 TC 12 Z9 13 U1 1 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0271-0749 J9 J CLIN PSYCHOPHARM JI J. Clin. Psychopharmacol. PD OCT PY 1995 VL 15 IS 5 BP 361 EP 364 DI 10.1097/00004714-199510000-00009 PG 4 WC Pharmacology & Pharmacy; Psychiatry SC Pharmacology & Pharmacy; Psychiatry GA RY196 UT WOS:A1995RY19600010 PM 8830068 ER PT J AU PEDROZO, HA SCHWARTZ, Z NAKAYA, H HARRISON, JL DEAN, DD WIEDERHOLD, ML BOYAN, BD AF PEDROZO, HA SCHWARTZ, Z NAKAYA, H HARRISON, JL DEAN, DD WIEDERHOLD, ML BOYAN, BD TI CARBONIC-ANHYDRASE IS REQUIRED FOR STATOCONIA HOMEOSTASIS IN ORGAN-CULTURES OF STATOCYSTS FROM APLYSIA-CALIFORNICA SO JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY LA English DT Article DE APLYSIA; CARBONIC ANHYDRASE; STATOCONIA; ORGAN CULTURE ID PARATHYROID-HORMONE; LOCALIZATION; OSTEOCLASTS; CALCITONIN AB A novel organ culture system has been developed to study the regulation of statoconia production in the gravity sensing organ in Aplysia californica. Statocysts were cultured in Leibovitz (L15) medium supplemented with salts and Aplysia haemolymph for four days at 17 degrees C. The viability of the system was evaluated by examining four parameters: statocyst morphology, the activity of the mechanosensory cilia in the statocyst, production of new statoconia during culture and change in statoconia volume after culture. There were no morphological differences in statocysts before and after culture when ciliary beating was maintained. There was a 29% increase in the number of statoconia after four days in culture. Mean statocyst, statolith and statoconia volumes were not affected by culture conditions. The presence of carbonic anhydrase in the statocysts was shown using immunohistochemistry. When statocysts were cultured in the presence of 4.0 x 10(-4) M acetazolamide to inhibit the enzyme activity, there was a decrease in statoconia production and statoconia volume, indicating a role for this enzyme in statoconia homeostasis, potentially via pH regulation. These studies are the first to report a novel system for the culture of statocysts and show that carbonic anhydrase is involved in the regulation of statoconia volume and production. C1 UNIV TEXAS,HLTH SCI CTR,DEPT ORTHOPAED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PERIODONT,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. HEBREW UNIV JERUSALEM,HADASSAH FAC DENT MED,DEPT PERIODONT,JERUSALEM,ISRAEL. OI Dean, David/0000-0002-4512-9065 FU NIDCR NIH HHS [DE05937] NR 23 TC 6 Z9 6 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0340-7594 J9 J COMP PHYSIOL A JI J. Comp. Physiol. A-Sens. Neural Behav. Physiol. PD OCT PY 1995 VL 177 IS 4 BP 415 EP 425 PG 11 WC Behavioral Sciences; Neurosciences; Physiology; Zoology SC Behavioral Sciences; Neurosciences & Neurology; Physiology; Zoology GA RU167 UT WOS:A1995RU16700004 PM 7674196 ER PT J AU KINOSIAN, B GLICK, H PREISS, L PUDER, KL AF KINOSIAN, B GLICK, H PREISS, L PUDER, KL TI CHOLESTEROL AND CORONARY HEART-DISEASE - PREDICTING RISKS IN MEN BY CHANGES IN LEVELS AND RATIOS SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Article DE CHOLESTEROL; CHD RISK; CORONARY PRIMARY PREVENTION TRIAL ID WOMEN; AREA AB Background: Little is known about the relative ability of different measures of change in cholesterol to discriminate coronary heart disease risk. We evaluated this ability for changes in low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, LDL/HDL ratios, and total cholesterol/HDL ratios. Methods: We predicted risks of coronary heart disease using data from 3641 men in the Lipid Research Clinics Coronary Primary Prevention Trial. Treating these patients as a cohort, we estimated risks associated with changes in cholesterol levels independent of the patients' randomization group. Results: Changes in LDL and HDL cholesterol when used in combination were each significant predictors of coronary heart disease risk (odds ratios [OR] for 10% increases, 1.15 and 0.84, respectively; P < 0.001). Changes in LDL/HDL and total cholesterol/HDL ratios had similar discriminating ability (OR for 10% increases, 1.17 and 1.21, respectively; P < 0.0001), In the best discriminating models, changes in ratios added information about risks to changes in LDL cholesterol, although changes in LDL cholesterol levels failed to add information to changes in ratios. Conclusions: Changes in total cholesterol/HDL and LDL/HDL ratios were better predictors of risk for coronary heart disease than were changes in LDL cholesterol levels alone. When assessed as percentage changes averaged during the first two months of intervention, they were among the best discriminators of risk. Clinicians selecting treatments for intervention should include among their considerations the treatment's effect on both LDL and HDL cholesterol rather than their effects on LDL cholesterol levels alone. C1 DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. COLUMBIA UNIV,NEW YORK,NY. SANDOZ INC,PHARMACEUT,E HANOVER,NJ 07936. RP KINOSIAN, B (reprint author), UNIV PENN,DIV GEN INTERNAL MED,RALSTON PENN CTR,ROOM 302,3615 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. FU NIA NIH HHS [R29-AG09837-08] NR 15 TC 80 Z9 82 U1 1 U2 3 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 1081-5589 J9 J INVEST MED JI J. Investig. Med. PD OCT PY 1995 VL 43 IS 5 BP 443 EP 450 PG 8 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA TE359 UT WOS:A1995TE35900004 PM 8528755 ER PT J AU NAGY, AK SHUSTER, TA AF NAGY, AK SHUSTER, TA TI ATP-BINDING PROTEINS ON THE EXTERNAL SURFACE OF SYNAPTIC PLASMA-MEMBRANES - IDENTIFICATION BY PHOTOAFFINITY-LABELING SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE EXTRACELLULAR ATP; PRESYNAPTIC ECTO-ATP-BINDING SITES; AZIDO-ATP BINDING ID TORPEDO ELECTRIC ORGAN; SARCOPLASMIC-RETICULUM; EXTRACELLULAR ATP; ECTO-ATPASE; SYNAPTOSOMES; 8-AZIDO-ATP; ADENOSINE; SITES; CELLS; HYDROLYSIS AB 8-Azidoadenosine triphosphate labeled in the alpha or gamma position with P-32 was used as a photoaffinity reagent for identifying ATP binding sites on the external surface of intact rat brain synaptosomes. As revealed by autoradiography of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns, UV irradiation of intact synaptosomes in the presence of the above radioactive compounds at 5-10 mu M resulted in the formation of several major radioactive conjugates with approximate molecular masses of 29, 45/46, 58, and 93 kDa, Minor bands of 20, 39, 52/54, 82/84, 120, and 140 kDa were also consistently labeled in these experiments. The possibility that labeling of these proteins was due to the presence of contaminating subcellular particles or intrasynaptosomal proteins was excluded. The major 8-azidoadenosine [alpha-P-32]triphosphate-labeled protein complex of similar to 45/46 kDa was resolved into several subbands that are labeled differently depending on the type of divalent cations added to the photoaffinity reaction, In the presence of magnesium only, the major labeled band appeared at 45 kDa. With calcium, two additional subbands (43 and 46 kDa) could be distinguished. In the presence of 1 mM EDTA, a band at 44 kDa was labeled within this ATP-binding complex, The labeling pattern of the subbands of this 45/46-kDa complex is consistent with these bands being extracellular ATP-binding proteins on the surface of the synaptosome. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. CALIF STATE UNIV LONG BEACH,DEPT BIOL SCI,LONG BEACH,CA 90840. FU NINDS NIH HHS [NS 21908] NR 42 TC 8 Z9 8 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD OCT PY 1995 VL 65 IS 4 BP 1849 EP 1858 PG 10 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA RW335 UT WOS:A1995RW33500049 PM 7561884 ER PT J AU SONG, MK ADHAM, NF HENG, MCY COSTEA, NV HENG, MK AMENT, ME AF SONG, MK ADHAM, NF HENG, MCY COSTEA, NV HENG, MK AMENT, ME TI METABOLIC ALTERATIONS OF ZINC AND PROSTAGLANDINS IN BOTH HUMAN AND ANIMAL COLONIC TUMOR-CELLS SO JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION LA English DT Article DE COLONIC NEOPLASMS; ZINC; PROSTAGLANDINS; RAT; PROSTAGLANDIN SYNTHASE ID LARGE-BOWEL CANCER; ASPIRIN USE; CROHNS-DISEASE; DIETARY ZINC; F344 RATS; RISK; GROWTH; CARCINOGENESIS; CYCLOOXYGENASE; DEFICIENCY AB Objective: To understand the relationship between zinc and prostaglandin (PG) metabolisms in inducing colon cancer incidence in human and animals. Methods: Human colonic tumor and normal cells were obtained from Departments of Surgery and Pathology at the Kaiser Permanente Medical Center, Los Angeles, CA and US VA Medical Center, North Hills, CA. Rat colonic tumor and normal cells were isolated from the rats that received two injections of 50 mg/kg of Azoxymethan (AOM) in 2 weeks and then kept 30 weeks in the animal facility. Then, the effects of zinc on the PGE(2) synthesis and PGE(2) on zinc metabolism in tumor and normal cells were determined. Results: PGE(2) concentrations in both human and AOM-induced rat colonic tumor cells increased compared to those in adjacent normal colonocytes, whereas PGF(2 alpha) concentrations did not change. Gene expression of inducible form of prostaglandin synthase (PGS-2) is stimulated in rat colonocytes by epidermal growth factor and by tetradecanoyl 13-phorbol acetate (a tumor promoter and mitogen) only in the presence of zinc, PGE(2) binding activity of rat enterocytes was maximum at 15 mu M of zinc (normal plasma zinc concentration), but PGE(2), synthesis activity increased for the first 15 minutes when extracellular zinc concentrations were either higher or lower than the normal extracellular zinc concentration. However, variations in extracellular zinc concentrations did not change the rate of PGF(2 alpha) synthesis in the normal rat enterocytes. PGE(2) significantly increased zinc uptake rates of colonic tumor cells but PGF(2 alpha) showed only moderate effect. Conclusions: These results suggest that zinc is required for PGS-2 gene expression, that maintaining an optimal zinc nutriture is important for normal PG synthesis of intestinal cells, and that only PGE(2) synthesis mechanisms rather than PGS-2 gene expression are altered in colonic tumor cells resulting in the abnormal zinc nutriture of these cells. C1 SEPULVEDA VA MED CTR, NORTH HILLS, CA USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 42 TC 2 Z9 2 U1 0 U2 0 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0731-5724 EI 1541-1087 J9 J AM COLL NUTR JI J. Am. Coll. Nutr. PD OCT PY 1995 VL 14 IS 5 BP 473 EP 479 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA RX307 UT WOS:A1995RX30700011 PM 8522726 ER PT J AU GHUSN, HF TEASDALE, TA SKELLY, JR AF GHUSN, HF TEASDALE, TA SKELLY, JR TI LIMITING TREATMENT IN NURSING-HOMES - KNOWLEDGE AND ATTITUDES OF NURSING-HOME MEDICAL DIRECTORS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Note ID CARDIOPULMONARY-RESUSCITATION; CARE; OUTCOMES; CPR; FACILITY AB OBJECTIVE: To determine nursing home medical directors' knowledge about cardiopulmonary resuscitation outcome and their support of treatment limitation requests and policies. DESIGN: Mailed questionnaire, followed by telephone interview. PARTICIPANTS: Forty-six medical directors of 70 community nursing homes in Harris County, Texas. MEASUREMENTS: Medical directors were asked to estimate the CPR survival rate to discharge of all nursing home residents and that of two case scenarios. They were asked to indicate on a Likert scale their support for mandatory Do-Not-Resuscitate orders and for requests by nursing home patients to withhold other life support measures. RESULTS: Responses were received from 33 directors. Overall CPR survival rate of older nursing home residents after cardiac arrest was thought to be 10.7%. The average CPR survival rate for healthy older people with witnessed arrests was believed to be 13.8%. The perceived rate for unwitnessed arrests in terminal patients was 4.6%, significantly lower than estimates for healthy older people (P = .003) and estimates of the overall survival rate (P = .02). Medical directors were split regarding mandatory Do-Not-Resuscitate orders for patients in vegetative states, with terminal illness, with an unwitnessed arrest, or in those older than 90 years of age. Mandatory use of Do-Not-Resuscitate orders for all nursing home residents was strongly opposed. Assuming a 2% survival rate did not significantly influence medical directors' opinions about mandatory DNR orders in these groups. Medical directors were more willing to support requests by stable nursing home residents to withhold resuscitation, mechanical ventilation, or hospitalization than requests to withhold antibiotics, intravenous fluids, or tube feedings (P < .005). The majority of medical directors were willing to withhold all such measures for terminal patients. CONCLUSIONS: Health care professionals who are responsible for educating patients about the efficacy of cardiopulmonary resuscitation in nursing homes overestimate its benefit and may benefit from further education about its outcome. Although mandatory Do-Not-Resuscitate orders were favored for terminal or vegetative patients, medical directors are not supportive of such orders across the board. Medical directors are more willing to honor requests for treatment limitation by terminal patients than others. C1 BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. RP GHUSN, HF (reprint author), HOUSTON VET AFFAIRS MED CTR,GERIATR & EXTENDED CARE SERV 110,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 13 TC 10 Z9 10 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD OCT PY 1995 VL 43 IS 10 BP 1131 EP 1134 PG 4 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RY285 UT WOS:A1995RY28500011 PM 7560705 ER PT J AU HUTCHISON, FN CUI, XY WEBSTER, SK AF HUTCHISON, FN CUI, XY WEBSTER, SK TI THE ANTIPROTEINURIC ACTION OF ANGIOTENSIN-CONVERTING ENZYME IS DEPENDENT ON KININ SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article DE PROTEINURIA; NEPHROTIC SYNDROME; BRADYKININ; KALLIKREIN; KININ ANTAGONIST ID PASSIVE HEYMANN NEPHRITIS; LIMITS GLOMERULAR INJURY; II RECEPTOR BLOCKADE; REDUCED RENAL MASS; BRADYKININ-ANTAGONIST; DIETARY-PROTEIN; INHIBITION; RATS; MODULATION; HYPERTENSION AB Converting enzyme inhibitors (CEI) reduce proteinuria in nephrotic humans and animals, but the mediator(s) of this effect has not been identified definitively. To determine whether enhanced kinin activity contributes to the antiproteinuric action of CEI, rats with passive Heymann nephritis were treated with the B-2 kinin receptor antagonist HOE 140, 300 mu g/kg per day, for 3 days and then the CEI enalapril (ENAL), 35 mg/kg per day, was given for another 4 days while HOE 140 was continued (HOE/ENAL). Additional groups of nephrotic rats were untreated (CON), received HOE 140 only (HOE), or received ENAL only. ENAL alone produced a >60% decrease in albuminuria after 4 days, whereas HOE 140 alone had no effect on albuminuria. In HOE/ENAL, pretreatment with HOE 140 prevented the decrease in albuminuria observed in ENAL. GFR increased significantly over time in all groups but was not different among the groups on any day. The clearance of albumin decreased significantly in ENAL (P <0.001) and was significantly lower than in CON, HOE, or HOE/ENAL on Day 10. The fractional clearance of albumin decreased in all groups as a result of the increase in GFR but was significantly lower in ENAL compared with the other three groups at Day 10 and was not different between CON, HOE, and HOE/ENAL. Plasma renin activity and concentration were increased significantly in both ENAL and HOE/ENAL, indicating that converting enzyme was effectively inhibited in both groups. It was concluded that enhanced kinin activity contributes to the antiproteinuric action of CEI in this model of nephrotic syndrome. C1 MED UNIV S CAROLINA,DIV NEPHROL,CHARLESTON,SC. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. FU NIDDK NIH HHS [DK-43185] NR 24 TC 35 Z9 36 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD OCT PY 1995 VL 6 IS 4 BP 1216 EP 1222 PG 7 WC Urology & Nephrology SC Urology & Nephrology GA TB912 UT WOS:A1995TB91200012 PM 8589289 ER PT J AU KIEBEREMMONS, T LIN, CM PRAMMER, KV VILLALOBOS, A KOSARI, F KLEYMAN, TR AF KIEBEREMMONS, T LIN, CM PRAMMER, KV VILLALOBOS, A KOSARI, F KLEYMAN, TR TI DEFINING TOPOLOGICAL SIMILARITIES AMONG ION-TRANSPORT PROTEINS WITH ANTI-AMILORIDE ANTIBODIES SO KIDNEY INTERNATIONAL LA English DT Article ID EPITHELIAL SODIUM-CHANNEL; EXCHANGER ISOFORM NHE-2; NA+/H+ EXCHANGER; EXPRESSION CLONING; DISTINCT EPITOPES; INTRACELLULAR PH; BINDING-PROTEIN; INHIBITION; ANALOGS; CDNA AB The structural features of amiloride binding sites an amiloride-sensitive transport proteins have received limited characterization. An antibody that recognizes limited regions of amiloride and can mimic, in binding specificity, certain amiloride-sensitive transport proteins was used as a model to elucidate potential amino acid residue relationships that might define putative amiloride contact sites. Analysis of the structure of this antibody has allowed us to identify sequence relationships among several Na+ selective transport proteins. A structure-based relational database was employed to re-examine sequence homologies among these ion transport proteins. A search of the protein sequence databank identified representative amino acid tracts among amiloride sensitive proteins involving planar residues that might be involved in interacting with amiloride. Computer models of sites within transmembrane domains of NHE1 and MHE2 isoforms of the Na+/H+ exchanger reflective of these planar tracts indicate that amiloride probably spans two helices for interaction with the Na+/H+ exchanger. Structural analysis of this monoclonal anti-amiloride antibody appears to mimic some of the salient features of amiloride binding sites on these proteins. C1 UNIV PENN,DEPT MED,PHILADELPHIA,PA 19104. UNIV PENN,DEPT BIOPHYS,PHILADELPHIA,PA 19104. UNIV PENN,DEPT PHYSIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP KIEBEREMMONS, T (reprint author), UNIV PENN,DEPT PATHOL & LAB MED,ROOM 267 JOHN MORGAN,PHILADELPHIA,PA 19104, USA. FU NHLBI NIH HHS [HL07027] NR 48 TC 15 Z9 15 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD OCT PY 1995 VL 48 IS 4 BP 956 EP 964 DI 10.1038/ki.1995.377 PG 9 WC Urology & Nephrology SC Urology & Nephrology GA RV926 UT WOS:A1995RV92600007 PM 8569105 ER PT J AU SCHUMACHER, HR HOWE, HS AF SCHUMACHER, HR HOWE, HS TI SYNOVIAL-FLUID CELLS IN SYSTEMIC LUPUS-ERYTHEMATOSUS - LIGHT AND ELECTRON-MICROSCOPIC STUDIES SO LUPUS LA English DT Article DE SYNOVIAL FLUID ID HUMAN-LYMPHOCYTES; INTERFERON-ALPHA; TUBULAR ARRAYS; INCLUSIONS; CISTERNAE; TUMOR; SLE AB Joint fluid findings in systemic lupus erythematosus (SLE) have been described in only a few series and systematic electron microscopic study of the synovial fluid (SF) cells has not been reported. We describe the evaluation of 17 SF in patients with SLE with routine analysis and electron microscopy. Joint effusions had a wider range of leukocyte counts than often appreciated, with counts varying from 875 to 39 250 cells per mm(3). LE inclusions were seen in eight fluids and have been shown to contain chromatin-like filaments by electron microscopy. There was little associated electron dense immunoglobulin-like material. Tubuloreticular structures (TRS) found in seven SF were mostly in mononuclear cells including some LE cells. The known association of TRS with alpha interferon and viral infections may have important implications for pathogenesis. C1 TANTOK SENG HOSP,SINGAPORE,SINGAPORE. RP SCHUMACHER, HR (reprint author), VET AFFAIRS MED CTR,ARTHRIT UNIT,39TH & WOODLAND AVE,PHILADELPHIA,PA 19104, USA. NR 76 TC 3 Z9 4 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0961-2033 J9 LUPUS JI Lupus PD OCT PY 1995 VL 4 IS 5 BP 353 EP 364 DI 10.1177/096120339500400505 PG 12 WC Rheumatology SC Rheumatology GA TB729 UT WOS:A1995TB72900005 PM 8563729 ER PT J AU MCCARTHY, MJ SHROYER, AL SETHI, GK MORITZ, TE HENDERSON, WG GROVER, FL LONDON, MJ GIBBS, JO LANSKY, D MILLER, D CLARK, J HAMMERMEISTER, KE AF MCCARTHY, MJ SHROYER, AL SETHI, GK MORITZ, TE HENDERSON, WG GROVER, FL LONDON, MJ GIBBS, JO LANSKY, D MILLER, D CLARK, J HAMMERMEISTER, KE TI SELF-REPORT MEASURES FOR ASSESSING TREATMENT OUTCOMES IN CARDIAC-SURGERY PATIENTS SO MEDICAL CARE LA English DT Article DE HEALTH STATUS ASSESSMENT; CARDIAC SURGERY ID HEALTH SURVEY SF-36; FUNCTIONAL STATUS; VALIDITY; QUALITY; LIFE AB Patient self-report measures are increasingly valued as outcome variables in health services research studies. In this article, the authors describe the Functional Status, Health Related Quality of Life, Life Satisfaction, and Patient Satisfaction scales included in the Processes, Structures, and Outcomes of Cardiac Surgery (PSOCS) cooperative study underway within the Department of Veterans Affairs health care system. In addition to reporting on the baseline psychometric characteristics of these instruments, the authors compared preoperative Medical Outcomes Study SF-36 data from the study patients with survey data from a probability sample of the US population and with preoperative data on cardiac surgery patients from a high volume private sector surgical practice. Descriptive analyses indicate that the SF-36 profiles for all of the cardiac patients are highly similar. The Veterans Affairs and private sector patients report diminished physical functioning, physical role functioning, and emotional role functioning as well as reduced energy relative to an age-matched comparison sample. At the same time, however, the Veterans Affairs patients evidenced lower levels of capacity on most of the SF-36 dimensions relative to the private sector patients. C1 VET AFFAIRS MED CTR,DEPT HLTH SERV,DENVER,CO. VET AFFAIRS MED CTR,DEPT SURG SERV,DENVER,CO. VET AFFAIRS MED CTR,DEPT ANESTHESIOL,DENVER,CO. VET AFFAIRS MED CTR,DEPT CARDIOL,DENVER,CO. UNIV COLORADO,SCH MED,DENVER,CO. VET AFFAIRS MED CTR,DEPT CARDIOTHORAC SURG,TUCSON,AZ. UNIV ARIZONA,ARIZONA HLTH SCI CTR,TUCSON,AZ. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,COOPERAT STUDIES PROGRAM COORDINATING CTR,HINES,IL 60141. NORTHWESTERN UNIV,CTR HLTH SERV & POLICY RES,EVANSTON,IL 60208. UNIV ILLINOIS,SCH PUBL HLTH,EPIDEMIOL & BIOSTAT PROGRAM,CHICAGO,IL. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,CTR COOPERAT STUDIES HLTH SERV,HINES,IL 60141. PROVIDENCE HLTH SYST,CLIN INFORMAT SYST,PORTLAND,OR. VET AFFAIRS HOSP,HLTH SERV RES & DEV FIELD PROGRAM,BEDFORD,MA. RP MCCARTHY, MJ (reprint author), NORTHWESTERN UNIV,SCH MED,DEPT PREVENT MED,SUITE 1102,680 LAKE SHORE DR,CHICAGO,IL 60611, USA. RI Shroyer, Annie Laurie/B-8836-2016 OI Shroyer, Annie Laurie/0000-0001-6461-0623 NR 39 TC 24 Z9 24 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD OCT PY 1995 VL 33 IS 10 SU S BP OS76 EP OS85 DI 10.1097/00005650-199510001-00009 PG 10 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TA873 UT WOS:A1995TA87300009 PM 7475415 ER PT J AU MORITZ, TE ELLIS, NK VILLANUEVA, CB STEEGER, JE LUDWIG, ST DEEGAN, NI SHROYER, AL HENDERSON, WG SETHI, GK GROVER, FL HAMMERMEISTER, KE AF MORITZ, TE ELLIS, NK VILLANUEVA, CB STEEGER, JE LUDWIG, ST DEEGAN, NI SHROYER, AL HENDERSON, WG SETHI, GK GROVER, FL HAMMERMEISTER, KE TI DEVELOPMENT OF AN INTERACTIVE DATA-BASE MANAGEMENT-SYSTEM FOR CAPTURING LARGE VOLUMES OF DATA SO MEDICAL CARE LA English DT Article DE DATA BASE MANAGEMENT; HIERARCHICAL DATA BASE; COMPUTER-ASSISTED INTERVIEW; DATA TRANSFER; DATA SECURITY AB Accurate collection and successful management of data are problems common to all scientific studies. For studies in which large quantities of data are collected by means of questionnaires and/or forms, data base management becomes quite laborious and time consuming. Data base management comprises data collection, data entry, data editing, and data base maintenance. In this article, the authors describe the development of an interactive data base management (IDM) system for the collection of more than 1,400 variables from a targeted population of 6,000 patients undergoing heart surgery requiring cardiopulmonary bypass. The goals of the IDM system are to increase the accuracy and efficiency with which this large amount of data is collected and processed, to reduce research nurse work load through automation of certain administrative and clerical activities, and to improve the process for implementing a uniform study protocol, standardized forms, and definitions across sites. C1 VET AFFAIRS MED CTR,CTR CONTINUOUS QUAL IMPROVEMENT,DENVER,CO. VET AFFAIRS MED CTR,RES DEPT,DENVER,CO. VET AFFAIRS MED CTR,DEPT HLTH SERV,DENVER,CO. VET AFFAIRS MED CTR,DEPT SURG SERV,DENVER,CO. VET AFFAIRS MED CTR,DEPT CARDIOL SERV,DENVER,CO. VET AFFAIRS MED CTR,BOSTON,MA. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV COLORADO,SCH MED,DENVER,CO. VET AFFAIRS MED CTR,DEPT CARDIOTHORAC SURG,TUCSON,AZ. UNIV ARIZONA,ARIZONA HLTH SCI CTR,TUCSON,AZ. RP MORITZ, TE (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,CTR COOPERAT STUDIES HLTH SERV,POB 5000,HINES,IL 60141, USA. RI Shroyer, Annie Laurie/B-8836-2016 OI Shroyer, Annie Laurie/0000-0001-6461-0623 NR 9 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD OCT PY 1995 VL 33 IS 10 SU S BP OS102 EP OS106 PG 5 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TA873 UT WOS:A1995TA87300011 ER PT J AU VILLANUEVA, CB LUDWIG, ST SHROYER, AL DEEGAN, NI STEEGER, JE LONDON, MJ SETHI, GK GROVER, FL HAMMERMEISTER, KE AF VILLANUEVA, CB LUDWIG, ST SHROYER, AL DEEGAN, NI STEEGER, JE LONDON, MJ SETHI, GK GROVER, FL HAMMERMEISTER, KE TI VARIATIONS IN THE PROCESSES AND STRUCTURES OF CARDIAC-SURGERY NURSING-CARE SO MEDICAL CARE LA English DT Article DE NURSING EDUCATION; NURSING STRUCTURE; NURSING PROCESS; PATIENT CARE OUTCOMES; MULTIDISCIPLINARY TEAM AB Nurses play an invaluable role as key members of the cardiac surgery patient's medical care team. Over the last century, the nursing profession has become more independent and autonomous. Despite the widespread use of nursing quality indicators, the effect of nursing-specific processes and structures of care on patient outcomes is unknown. Thus, the Processes, Structures, and Outcomes of Care in Cardiac Surgery (PSOCS) study was initiated, in part, to determine the potential effect of nursing processes and structures of care on cardiac surgery patients' risk-adjusted outcomes. In this article, the authors summarize the key components of nursing structures of care incorporated in the PSOCS study. Nursing process variables were not sufficiently designed into the study to address hypotheses relating nursing care processes to patient outcomes. An analysis of the pilot test data from September 1992 to September 1993 demonstrated potentially important variations between the six pilot centers regarding nursing care provider profiles leg, educational preparation, specialty certification, and experience levels) and nursing staff ratios (eg, within the surgical intensive care unit). When linked to risk-adjusted patient outcomes, these variations in nursing structure of care may offer important insights toward improving the quality of care of cardiac surgery patients. C1 VET AFFAIRS MED CTR,DEPT HLTH SERV,DENVER,CO 80220. VET AFFAIRS MED CTR,DEPT ANESTHESIOL,DENVER,CO 80220. VET AFFAIRS MED CTR,DEPT SURG SERV,DENVER,CO 80220. VET AFFAIRS MED CTR,DEPT CARDIOL,DENVER,CO 80220. UNIV COLORADO,SCH MED,DENVER,CO. VET AFFAIRS MED CTR,PROC STRUCT & OUTCOMES CARE CARDIAC SURG STUDY,SAN ANTONIO,TX. VET AFFAIRS MED CTR,PROC STRUCT & OUTCOMES CARE CARDIAC SURG STUDY,BOSTON,MA. VET AFFAIRS MED CTR,TUCSON,AZ. UNIV ARIZONA,ARIZONA HLTH SCI CTR,TUCSON,AZ. RP VILLANUEVA, CB (reprint author), VET AFFAIRS MED CTR,PSOCS STUDY,1055 CLERMONT ST,DENVER,CO 80220, USA. RI Shroyer, Annie Laurie/B-8836-2016 OI Shroyer, Annie Laurie/0000-0001-6461-0623 NR 13 TC 1 Z9 1 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD OCT PY 1995 VL 33 IS 10 SU S BP OS59 EP OS65 DI 10.1097/00005650-199510001-00007 PG 7 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA TA873 UT WOS:A1995TA87300007 PM 7475413 ER PT J AU PONTON, MO DARCOURT, J MILLER, BL CUMMINGS, JL SCHUMANN, SW MAEN, I AF PONTON, MO DARCOURT, J MILLER, BL CUMMINGS, JL SCHUMANN, SW MAEN, I TI PSYCHOMETRIC AND SPECT STUDIES IN ALZHEIMERS-DISEASE WITH AND WITHOUT DELUSIONS SO NEUROPSYCHIATRY NEUROPSYCHOLOGY AND BEHAVIORAL NEUROLOGY LA English DT Article DE ALZHEIMERS DISEASE; PSYCHOMETRIC MEASURE; SPECT; DELUSIONS ID CEREBRAL BLOOD-FLOW; EMISSION COMPUTED-TOMOGRAPHY; CAPGRAS SYNDROME; SINGLE-PHOTON; COGNITIVE PERFORMANCE; DEMENTIA; PERFUSION; BRAIN; MISIDENTIFICATION; HALLUCINATIONS AB Delusions are common in Alzheimer's disease (AD), but their pathophysiological basis is poorly understood. Fifteen patients meeting NINCDS-ADRDA criteria for probable AD were studied with psychometric assessment and brain-dedicated SPECT (xenon-133 and HMPAO). None of the subjects had delusions at the start of the study. One year later, psychometric testing and SPECT were repeated. Three patients had developed Capgras syndrome, and three others had developed non-Capgras delusional disorders in the interval. Psychometric and rCBF measures at baseline and at one year were compared. At baseline rCBF was significantly higher in the right temporal regions in the patients who developed delusions (p < 0.05). One year later, rCBF in the right temporal regions had declined in the psychotic patients. AD patients who developed delusions showed deterioration in right anterior temporal rCBF compared to AD without delusions. In the setting of AD, right temporal function deterioration may contribute to the development of delusions. C1 UNIV NICE,NICE,FRANCE. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. RP PONTON, MO (reprint author), UNIV CALIF LOS ANGELES,HARBOR MED CTR,DEPT NEUROL,BLDG F9,1000 W CARSON ST,TORRANCE,CA 90509, USA. NR 63 TC 25 Z9 25 U1 1 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0894-878X J9 NEUROPSY NEUROPSY BE JI Neuropsychiatr. Neuropsychol. Behav. Neurol. PD OCT PY 1995 VL 8 IS 4 BP 264 EP 270 PG 7 WC Clinical Neurology; Psychiatry; Psychology SC Neurosciences & Neurology; Psychiatry; Psychology GA RZ144 UT WOS:A1995RZ14400004 ER PT J AU MENDEZ, MF VANGORP, W CUMMINGS, JL AF MENDEZ, MF VANGORP, W CUMMINGS, JL TI NEUROPSYCHIATRY, NEUROPSYCHOLOGY, AND BEHAVIORAL NEUROLOGY - A CRITICAL COMPARISON SO NEUROPSYCHIATRY NEUROPSYCHOLOGY AND BEHAVIORAL NEUROLOGY LA English DT Note ID ALZHEIMERS-DISEASE; SCHIZOPHRENIA; DISORDERS; EPILEPSY; BRAIN; MOOD AB Neuropsychiatry, neuropsychology, and behavioral neurology share similar interests in brain-behavior disturbances. Recent advances in neuroscience have encouraged an increasing convergence of the three disciplines. These fields have differences in theoretical basis, professional and developmental background, most common brain-behavior focus, and principle clinical methods and therapies. This article compares neuropsychiatry, neuropsychology, and behavioral neurology and suggests how improved interdisciplinary knowledge and interactions can lead to greater understanding of the neurobiologic basis of behavior. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHOL SERV,LOS ANGELES,CA 90073. NR 40 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0894-878X J9 NEUROPSY NEUROPSY BE JI Neuropsychiatr. Neuropsychol. Behav. Neurol. PD OCT PY 1995 VL 8 IS 4 BP 297 EP 302 PG 6 WC Clinical Neurology; Psychiatry; Psychology SC Neurosciences & Neurology; Psychiatry; Psychology GA RZ144 UT WOS:A1995RZ14400009 ER PT J AU JAINKITTIVONG, A YEH, CK GUEST, GF COTTONE, JA AF JAINKITTIVONG, A YEH, CK GUEST, GF COTTONE, JA TI EVALUATION OF MEDICAL CONSULTATIONS IN A PREDOCTORAL DENTAL CLINIC SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Article AB Objective. A patient's medical condition can affect the delivery of dental care. Medical consultation is indicated for dental patients whose medical history is uncertain or when physical assessment may indicate an untreated medical problem. The aims of this study were to evaluate the use of medical consultation and determine how it affects dental treatment plans in a predoctoral dental clinic program. Study design. Reviews of 147 medical consultation requests were performed. Results. The main reasons for medical consultations were cardiovascular assessment (51.5%) and diabetic status determination (12.6%). In the cardiovascular assessment category, hypertension (48.1%) and heart murmur (17.9%) were primary concerns. Main dental concerns were the need for preoperative antibiotic prophylaxis (33.3%) and the use of vasoconstrictors (20.4%). Overall, 32.1% of medical consultations resulted in an alteration in dental treatment plans. As a result of medical consultations, 8% commenced their medical management. Conclusions. These results indicated that medical consultations could reduce the medical risk associated with dental procedures and unnecessary antibiotic prophylaxis. Therefore for many dental patients, good communication between dentists and physicians is essential for adequate care. C1 UNIV TEXAS,HLTH SCI CTR,SCH DENT,DEPT DENT DIAGNOST SCI,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284. NR 9 TC 5 Z9 5 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD OCT PY 1995 VL 80 IS 4 BP 409 EP 413 PG 5 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TA766 UT WOS:A1995TA76600012 PM 8521104 ER PT J AU GULLEY, ML SARGEANT, KP GRIDER, DJ EAGAN, PA DAVEY, DD DAMM, DD ROBINSON, RA VANDERSTEEN, DP MCGUFF, S BANKS, PM AF GULLEY, ML SARGEANT, KP GRIDER, DJ EAGAN, PA DAVEY, DD DAMM, DD ROBINSON, RA VANDERSTEEN, DP MCGUFF, S BANKS, PM TI LYMPHOMAS OF THE ORAL SOFT-TISSUES ARE NOT PREFERENTIALLY ASSOCIATED WITH LATENT OR REPLICATIVE EPSTEIN-BARR-VIRUS SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Article ID NON-HODGKINS-LYMPHOMAS; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; T-CELL LYMPHOMAS; MORPHOLOGICAL SUBDIVISION; CLINICAL-SIGNIFICANCE; GENE-EXPRESSION; DNA; LEUKOPLAKIA; DISEASE; RNAS AB Objectives. Epstein-Barr virus is periodically shed in the saliva of persons infected by the virus.Epstein-Barr virus has been implicated in the pathogenesis of certain subtypes of lymphoma, particularly high-grade lymphomas. Because high-grade subtypes represent the majority of lymphomas that arise in oral soft tissues, we hypothesized that Epstein-Barr virus might be preferentially associated with oral lymphomas. Study Design, A series of 34 oral lymphomas were diagnosed according to the revised European-American classification scheme. They were examined for the presence of latent Epstein-Barr virus by EBER1 in situ hybridization and for expression of the Epstein-Barr virus replicative protein, BZLF1, by immunohistochemistry. Results, Epstein-Barr virus EBER1 transcripts were detected in 11 of 31 oral lymphomas including 7 of 10 AIDS-related lymphomas and only 4 of 21 lymphomas that occurred in nonimmunocompromised persons. The Epstein-Barr virus-containing lymphomas were art high-grade histologic subtypes, that is, diffuse large cell, immunoblastic, or Burkitt's lymphomas. In contrast, Epstein-Barr virus was not detected in any of five tow-grade oral lymphomas. in the single case of T-cell lymphoma in this study, EBER1 was expressed in the tumor cells. A switch from viral latency to replication, as measured by EBV BZLF1 expression, was identified in rare lymphoma cells in only four cases. This rate of viral replication was not higher than what has been reported in lymphomas arising at other anatomic sites. Although one of our lymphomas arose at a site oi previous oral hairy leukoplakia, there was no other evidence that Epstein-Barr virus replication predisposed to development or persistence of oral lymphomas. Conclusions. These data suggest that even though Epstein-Barr virus is frequently found in oral secretions, neither latent nor replicative Epstein-Barr virus is present more commonly in oral lymphomas than in lymphomas arising in other anatomic sites, when controlling for immunodeficiency status. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. BROOKE ARMY MED CTR,FT SAM HOUSTON,TX 78234. UNIV KENTUCKY,MED CTR,LEXINGTON,KY 40506. UNIV IOWA HOSP & CLIN,IOWA CITY,IA 52242. WILFORD HALL USAF MED CTR,LACKLAND AFB,TX. RP GULLEY, ML (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NCI NIH HHS [K08-CA01615] NR 35 TC 15 Z9 15 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD OCT PY 1995 VL 80 IS 4 BP 425 EP 431 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TA766 UT WOS:A1995TA76600015 PM 8521106 ER PT J AU NETSCHER, DT PATRINELY, JR PELTIER, M POLSEN, C THORNBY, J AF NETSCHER, DT PATRINELY, JR PELTIER, M POLSEN, C THORNBY, J TI TRANSCONJUNCTIVAL VERSUS TRANSCUTANEOUS LOWER EYELID BLEPHAROPLASTY - A PROSPECTIVE-STUDY SO PLASTIC AND RECONSTRUCTIVE SURGERY LA English DT Article ID LOWER-LID BLEPHAROPLASTY; ORBITAL FRACTURES; INCISIONS AB Debate continues over the relative merits of transconjunctival and the more customary subciliary transcutaneous approaches for lower lid blepharoplasty. Tell consecutive patients presented for blepharoplasty, and in all patients the transcutaneous subciliary musculocutaneous flap approach was used on the left lower eyelid and the transconjunctival preseptal approach was used on the right. Patients served as their own controls. Followup was evaluated clinically by patient questionnaire and by standardized photographs preoperatively and at 5 days, 1 month, 3 months, and 6 months postoperatively. Photographs were graded independently by four blinded examiners. No statistical difference was identified in measured fornix depth between preoperative patients and postoperatively on each side. Average fat removed from each side was the same, and no patient had an identified ''missed fat compartment.'' Three patients had mild bilateral scleral show postoperatively, and a fourth developed it on the left (transcutaneous) side. However, overall grading on both sides was universally very good with no significant difference on the two sides-0.68 on the right and 0.60 on the left (maximum worst grade could be 5.0 and best grade 0). The potential for external scarring was never a perceived problem in the transcutaneous technique. C1 DEPT VET AFFAIRS MED CTR,PLAST SURG SERV,HOUSTON,TX. BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. NR 22 TC 26 Z9 26 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0032-1052 J9 PLAST RECONSTR SURG JI Plast. Reconstr. Surg. PD OCT PY 1995 VL 96 IS 5 BP 1053 EP 1060 DI 10.1097/00006534-199510000-00007 PG 8 WC Surgery SC Surgery GA RX593 UT WOS:A1995RX59300007 PM 7568479 ER PT J AU NETSCHER, DT WEIZER, G WIGODA, P WALKER, LE THORNBY, J BOWEN, D AF NETSCHER, DT WEIZER, G WIGODA, P WALKER, LE THORNBY, J BOWEN, D TI CLINICAL RELEVANCE OF POSITIVE BREAST PERIPROSTHETIC CULTURES WITHOUT OVERT INFECTION SO PLASTIC AND RECONSTRUCTIVE SURGERY LA English DT Article ID CAPSULAR CONTRACTURE; IMPLANTS; COLONIZATION AB The true incidence of positive breast periprosthetic cultures in the absence of overt infection is not clearly established. We retrospectively reviewed data fr om 389 implants that were removed for reasons other than clinical infection. Many of these patients presented with a variety of musculoskeletal ailments. Others had symptomatic capsular contracture as the presenting complaint In a few a known implant rupture was the reason for explantation. We identified a positive culture rate of 23.5 percent from capsule tissue. Most of these organisms were coagulase-negative staphylococci and anaerobic diphtheroids, but fungi and other organisms (generally felt to be more pathogenic than the less virulent coagulase-negative staphylococci) also were cultured. In an attempt to identify the clinical relevance of these positive cultures, we statistically evaluated the culture results for associations with capsular contracture, implant rupture, type of implant, and location of implant. Of these, the only statistically significant correlation was between positive culture result and symptomatic capsular contracture (Baker class IV). C1 BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. UNIV TEXAS,SW MED CTR,DEPT SURG,DALLAS,TX 75235. DEPT VET AFFAIRS MED CTR,HOUSTON,TX. NR 25 TC 56 Z9 56 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0032-1052 J9 PLAST RECONSTR SURG JI Plast. Reconstr. Surg. PD OCT PY 1995 VL 96 IS 5 BP 1125 EP 1129 DI 10.1097/00006534-199510000-00020 PG 5 WC Surgery SC Surgery GA RX593 UT WOS:A1995RX59300020 PM 7568489 ER PT J AU TYAN, ML AF TYAN, ML TI EFFECTS OF VITAMIN-A AND INOSITOL ON TERM WEIGHTS OF H-2 CONGENIC MICE SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Article ID PLACENTAL-LACTOGEN RELEASE; HUMAN TROPHOBLASTIC CELLS; PHOSPHATE PRODUCTION; GROWTH; METABOLISM; ENDOCRINE; FETUS AB Pregnant H-2 congenic mice, C57BL/10, B10.A, B10.A(15R), and B10.A(18R) were fed Purina Laboratory Chow 5001 or the same diet supplemented with 400 IU vitamin A dissolved in corn oil or 0.4% (w/w) myo-inositol, On the 18th day of gestation, the dams were sacrificed, and the fetuses were weighed, sexed, and examined for developmental abnormalities. Term fetal weight was found to be significantly reduced in progeny of darns bearing d alleles distal to Ea in the H-2 complex when the diet was supplemented with vitamin A or myo-inositol (B10.A and B10.A [18R]). Fetuses from dams of all strains fed the diet supplemented with vitamin A had significantly increased frequencies of microphthalmia; the frequency of microphthalmia was moderately but not significantly increased in one of the two strains fed the diet supplemented with myo-inositol (B10.A[15R] but not B10.A[18R]). RP TYAN, ML (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,111M,LOS ANGELES,CA 90073, USA. NR 23 TC 1 Z9 1 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD OCT PY 1995 VL 210 IS 1 BP 77 EP 80 PG 4 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA RV747 UT WOS:A1995RV74700012 PM 7675802 ER PT J AU TREVINO, RJ HUNT, J HOROWITZ, PM CHIRGWIN, JM AF TREVINO, RJ HUNT, J HOROWITZ, PM CHIRGWIN, JM TI CHINESE-HAMSTER RHODANESE CDNA - ACTIVITY OF THE EXPRESSED PROTEIN IS NOT BLOCKED BY A C-TERMINAL EXTENSION SO PROTEIN EXPRESSION AND PURIFICATION LA English DT Article ID BOVINE LIVER RHODANESE; SEQUENCE-ANALYSIS; RNA-POLYMERASE; CLONING; GENES AB We describe a cDNA from Chinese hamster ovary cells which encodes a protein 91 and 96% identical to bovine and rat mitochondrial rhodaneses, respectively. Recombinant protein was expressed from the cDNA in Escherichia coli, purified to homogeneity, and found to have kinetic properties nearly indistinguishable from those of the bovine enzyme, the only cloned rhodanese previously verified by characterization of the recombinant protein, The carboxyl-terminus of the enzyme is characterized by a duplicated tripeptide, which can be proteolytically processed in vivo. We constructed a mutant in which the last 5 amino acids were replaced by 28 residues of unrelated sequence. This protein was expressed, purified, and found to have kinetic constants similar to those of the wild-type enzyme. The functionally verified Chinese hamster rhodanese cDNA encodes a protein of 297 residues and differs from the rat enzyme at 13 positions. None of these substitutions occurs at residues suggested to play essential roles in catalysis or structural stabilization. (C) 1995 Academic Press, Inc. C1 UNIV TEXAS, HLTH SCI CTR, DEPT MED, DIV ENDOCRINOL, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT BIOCHEM, SAN ANTONIO, TX 78284 USA. AUDIE L MURPHY MEM VET ADM MED CTR, RES SERV, SAN ANTONIO, TX 78284 USA. FU NIEHS NIH HHS [ES 05729]; NIGMS NIH HHS [GM 25177] NR 31 TC 4 Z9 6 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1046-5928 EI 1096-0279 J9 PROTEIN EXPRES PURIF JI Protein Expr. Purif. PD OCT PY 1995 VL 6 IS 5 BP 693 EP 699 DI 10.1006/prep.1995.1091 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA RW190 UT WOS:A1995RW19000018 PM 8535164 ER PT J AU MORRELL, MJ BADR, MS HARMS, CA DEMPSEY, JA AF MORRELL, MJ BADR, MS HARMS, CA DEMPSEY, JA TI THE ASSESSMENT OF UPPER AIRWAY PATENCY DURING APNEA USING CARDIOGENIC OSCILLATIONS IN THE AIR-FLOW SIGNAL SO SLEEP LA English DT Article DE PULSE ARTIFACT; CENTRAL APNEA; NREM SLEEP; UPPER AIRWAY ID SLEEP; DOGS AB We investigated the relationship between airway patency and the occurrence of cardiogenic related oscillations in the airflow signal during 67 apneas occurring in non-rapid eye movement sleep in eight subjects. Spontaneously occurring apneas and apneas induced by mechanical ventilation were analyzed, Airway occlusion was determined by direct observation of the pharyngeal lumen using fiberoptic endoscopy. The presence or absence of cardiogenic oscillations was determined from an expanded airflow signal by an investigator blinded to the airway patency. Of the total 67 apneas, complete airway occlusion occurred during 51, and the airway remained patent throughout in 16. Cardiogenic oscillations were seen throughout 39 of the 51 occluded apneas and throughout 9 of the 16 apneas with the airway patent. There was no relationship between the occurrence of cardiogenic oscillations and airway patency. In addition, in a canine model where the upper airway was anatomically isolated, cardiogenic oscillations were evident during apneas in pressure signals recorded from the isolated upper airway and in airflow signals at the tracheal stoma. We conclude that cardiogenic oscillations cannot be used to predict airway patency during apnea. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,MADISON,WI. RP MORRELL, MJ (reprint author), UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,JOHN RANKIN PULM MED LAB,504 N WALNUT ST,MADISON,WI 53705, USA. FU NHLBI NIH HHS [HL 02588] NR 8 TC 17 Z9 17 U1 0 U2 1 PU AMER SLEEP DISORDERS ASSOC PI ROCHESTER PA 1610 14TH STREET NW SUITE 300, ROCHESTER, MN 55806 SN 0161-8105 J9 SLEEP JI Sleep PD OCT PY 1995 VL 18 IS 8 BP 651 EP 658 PG 8 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA TD542 UT WOS:A1995TD54200005 PM 8560131 ER PT J AU SHEKELLE, P MARKOVICH, M LOUIE, R AF SHEKELLE, P MARKOVICH, M LOUIE, R TI UNTITLED - RESPONSE SO SPINE LA English DT Letter C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP SHEKELLE, P (reprint author), RAND CORP,SANTA MONICA,CA 90406, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0362-2436 J9 SPINE JI SPINE PD OCT 1 PY 1995 VL 20 IS 19 BP 2172 EP 2173 DI 10.1097/00007632-199510000-00023 PG 2 WC Clinical Neurology; Orthopedics SC Neurosciences & Neurology; Orthopedics GA RX913 UT WOS:A1995RX91300023 ER PT J AU YOSHIMURA, M HERSHMAN, JM AF YOSHIMURA, M HERSHMAN, JM TI THYROTROPIC ACTION OF HUMAN CHORIONIC-GONADOTROPIN SO THYROID LA English DT Review ID NORMAL PREGNANT-WOMEN; THYROID-STIMULATING ACTIVITY; HUMAN TSH RECEPTORS; HYPEREMESIS GRAVIDARUM; LUTEINIZING-HORMONE; SIGNAL TRANSDUCTION; HYDATIDIFORM MOLES; MORNING SICKNESS; CELLS; HCG AB Hyperthyroidism or increased thyroid function has been reported in many patients with trophoblastic tumors. In these cases, greatly increased human chorionic gonadotropin (hCG) levels and suppressed TSH levels suggest that hCG has thyrotropic activity. Recent investigations have clarified the structural homology not only in the hCG and TSH molecules but also in their receptors, and this homology suggests the basis for the reactivity of hCG with the TSH receptor. The clinical significance of the thyrotropic action of hCG is now also recognized in normal pregnancy and hyperemesis gravidarum. Highly purified hLH binds to recombinant hTSH receptor and is about 10 times as potent as purified hCG in increasing cAMP. The beta-subunits of hCG and hLH share 85% sequence identity in their first 114 amino acids but differ in the carboxy-terminal peptide because hCG beta contains a 31-amino acid extension (beta-CTP). A recombinant mutant hCG that lacks beta-CTP showed almost identical potency to LH on stimulation of recombinant hTSH receptor. If intact hCG were as potent as hLH in regard to its thyrotropic activity, most pregnant women would become thyrotoxic. One of the roles of the beta-CTP may be to prevent overt hyperthyroidism in the first trimester of pregnancy when a large amount of hCG is produced by the placenta. Nicked hCG preparations, obtained from patients with trophoblastic disease or by enzymatic digestion of intact hCG, showed approximately 1.5- to 2-fold stimulation of recombinant hTSH receptor compared with intact hCG. This suggests that the thyrotropic activity of hCG may be influenced by the metabolism of the hCG molecule itself. Deglycosylation and/or desialylation of hCG enhances its thyrotropic potency. Basic hCG isoforms with lower sialic acid content extracted from hydatidiform moles were more potent in activating adenylate cyclase, and showed high bioactivity/immunoactivity (B/I) ratio in CHO cells expressing human TSH receptors. This is consistent with the finding that the beta-CTP truncated hCG with higher thyrotropic potency is substantially deglycosylated and desialylated in the beta-subunit relative to intact hCG because all four O-linked glycosylation sites occur within the missing C-terminal extension. The desialylated hCG variant also interacts directly with recombinant hTSH receptors transfected into human thyroid cancer cells. There is thyroid-stimulating activity in sera of normal pregnant women, and this correlates with serum hCG levels. The thyroid gland of normal pregnant women may be stimulated by hCG to secrete slightly excessive quantities of T-4 and induce a slight suppression of TSH, perhaps being about 1 mU/L less than nongravid levels, but not high enough to induce overt hyperthyroidism. Maternal thyroid glands may secrete more thyroid hormone during early pregnancy in response to the thyrotropic activity of hCG that overrides the normal operation of the hypothalamic-pituitary-thyroid feedback system. Biochemical hyperthyroidism associated with hyperemesis gravidarum has been attributed to hCG. In patients with hyperemesis gravidarum, thyrotropic activity in serum correlated with hCG immunoreactivity, and the severity of vomiting as indicated by clinical and biochemical parameters correlated with the degree of thyroid stimulation. To understand the thyrotropic action of hCG, it is necessary to know whether hCG activates the same domain of the TSH receptor as does TSH. The identification of the molecular structure of the hCG isoform with the highest thyrotropic potency will resolve the enigma of gestational thyrotoxicosis and the hyperthyroidism associated with trophoblastic disease and hCG-producing tumors. C1 W LOS ANGELES VET AFFAIRS MED CTR,DIV ENDOCRINOL & METAB,LOS ANGELES,CA 90073. KANSAI MED UNIV,DEPT INTERNAL MED 2,OSAKA,JAPAN. NR 61 TC 143 Z9 147 U1 0 U2 5 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1050-7256 J9 THYROID JI Thyroid PD OCT PY 1995 VL 5 IS 5 BP 425 EP 434 DI 10.1089/thy.1995.5.425 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TF020 UT WOS:A1995TF02000014 PM 8563483 ER PT J AU UBEL, PA ARNOLD, RM AF UBEL, PA ARNOLD, RM TI THE UNBEARABLE RIGHTNESS OF BEDSIDE RATIONING - PHYSICIAN DUTIES IN A CLIMATE OF COST-CONTAINMENT SO ARCHIVES OF INTERNAL MEDICINE LA English DT Article ID HEALTH-CARE; RESOURCE-ALLOCATION; PRACTICE GUIDELINES; OREGON; INTERVENTIONS; INEVITABILITY; OSMOLALITY; DECISIONS; DOCTORS; QUALITY AB A local internist is in the process of ordering an intravenous pyelogram for a patient she suspects of having kidney problems, when a medical student shadowing her in clinic interrupts. The student wants to know why the physician is not ordering a low-osmolality contrast agent for the patient, having read that they are less likely to cause serious side effects than high-osmolality contrast agents, The physician realizes that: the medical student is correct, but rejects the suggestion, telling the student that ''low-osmolality contrast agents are the standard of care for low-risk patients.'' C1 UNIV PENN,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. UNIV PENN,CTR BIOETH,PHILADELPHIA,PA 19104. UNIV PENN,LEONARD DAVIS INST HLTH ECON,PHILADELPHIA,PA 19104. UNIV PITTSBURGH,DIV GEN INTERNAL MED,PITTSBURGH,PA. UNIV PITTSBURGH,CTR MED ETH,PITTSBURGH,PA. RP UBEL, PA (reprint author), VET AFFAIRS MED CTR,111GM,UNIV & WOODLAND AVE,PHILADELPHIA,PA 19104, USA. NR 50 TC 52 Z9 52 U1 1 U2 1 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9926 J9 ARCH INTERN MED JI Arch. Intern Med. PD SEP 25 PY 1995 VL 155 IS 17 BP 1837 EP 1842 DI 10.1001/archinte.155.17.1837 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA RV804 UT WOS:A1995RV80400002 PM 7677549 ER PT J AU SHANER, A ECKMAN, TA ROBERTS, LJ WILKINS, JN TUCKER, DE TSUANG, JW MINTZ, J AF SHANER, A ECKMAN, TA ROBERTS, LJ WILKINS, JN TUCKER, DE TSUANG, JW MINTZ, J TI DISABILITY INCOME, COCAINE USE, AND REPEATED HOSPITALIZATION AMONG SCHIZOPHRENIC COCAINE ABUSERS - A GOVERNMENT-SPONSORED REVOLVING-DOOR SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID ADDICTION SEVERITY INDEX; SUBSTANCE-ABUSE; DRUG-ABUSE; PSYCHIATRIC-PATIENTS; URINE; CHROMATOGRAPHY; VALIDITY AB Background. Many patients with serious mental illness are addicted to drugs and alcohol. This comorbidity creates additional problems for the patients and for the clinicians, health care systems, and social-service agencies that provide services to this population. One problem is that disability income, which many people with serious mental illness receive to pay for basic needs, may facilitate drug abuse. In this study, we assessed the temporal patterns of cocaine use, psychiatric symptoms, and psychiatric hospitalization in a sample of schizophrenic patients receiving disability income. Methods. We evaluated 105 male patients with schizophrenia and cocaine dependence at the time of their admission to the hospital. They had severe mental illness and a long-term dependence on cocaine, with repeated admissions to psychiatric hospitals; many were homeless. The severity of psychiatric symptoms and urinary concentrations of the cocaine metabolite benzoylecgonine were evaluated weekly for 15 weeks. Results. Cocaine use, psychiatric symptoms, and hospital admissions all peaked during the first week of the month, shortly after the arrival of the disability payment, on the first day. The average patient spent nearly half his total income on illegal drugs. Conclusions. Among cocaine-abusing schizophrenic persons, the cyclic pattern of drug use strongly suggests that it is influenced by the monthly receipt of disability payments. The consequences of this cycle include the depletion of funds needed for housing and food, exacerbation of psychiatric symptoms, more frequent psychiatric hospitalization, and a high rate of homelessness. The troubling irony is that income intended to compensate for the disabling effects of severe mental illness may have the opposite effect. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. RP SHANER, A (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,116A,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. FU NIMH NIH HHS [R01 MH48081] NR 39 TC 148 Z9 148 U1 4 U2 12 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD SEP 21 PY 1995 VL 333 IS 12 BP 777 EP 783 DI 10.1056/NEJM199509213331207 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA RU809 UT WOS:A1995RU80900007 PM 7643886 ER PT J AU MAHONEY, JE GRAY, SL CARNES, M AF MAHONEY, JE GRAY, SL CARNES, M TI PREVENTION AND TREATMENT OF THE COMPLICATIONS OF DIABETES-MELLITUS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter ID HIP FRACTURE; RISK C1 UNIV WISCONSIN,SCH PHARM,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. RP MAHONEY, JE (reprint author), UNIV WISCONSIN,SCH MED,MADISON,WI 53706, USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD SEP 21 PY 1995 VL 333 IS 12 BP 802 EP 802 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA RU809 UT WOS:A1995RU80900021 PM 7643897 ER PT J AU IVESTER, CT TUXWORTH, WJ COOPER, G MCDERMOTT, PJ AF IVESTER, CT TUXWORTH, WJ COOPER, G MCDERMOTT, PJ TI CONTRACTION ACCELERATES MYOSIN HEAVY-CHAIN SYNTHESIS RATES IN ADULT CARDIOCYTES BY AN INCREASE IN THE RATE OF TRANSLATIONAL INITIATION SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID AORTIC PERFUSION-PRESSURE; PROTEIN-SYNTHESIS; CARDIAC-HYPERTROPHY; GENE-EXPRESSION; FELINE CARDIOCYTES; SKELETAL-MUSCLE; LOAD REGULATION; MESSENGER-RNAS; HEART-CELLS; OVERLOAD AB The purpose of this study was to determine the mechanism by which contraction acutely accelerates the synthesis rate of the contractile protein myosin heavy chain (MHC). Laminin-adherent adult feline cardiocytes were maintained in a serum-free medium and induced to contract at 1 Hz via electrical field stimulation. Electrical stimulation of contraction accelerated rates of MHC synthesis 28%, p < 0.05 by 4 h as determined by incorporation of [H-3]phenylalanine into MHC. MHC mRNA expression as measured by RNase protection was unchanged after 4 h of electrical stimulation. MHC mRNA levels in messenger ribonucleoprotein complexes and translating polysomes were examined by sucrose gradient fractionation. The relative percentage of polysome-bound MHC mRNA was equal at 47% in both electrically stimulated and control cardiocytes. However, electrical stimulation of contraction resulted in a reproducible shift of MHC mRNA from smaller polysomes into larger polysomes, indicating an increased rate of initiation. This shift resulted in significant increases in MHC mRNA levels in the fractions containing the larger polysomes of electrically stimulated cardiocytes as compared with nonstimulated controls. These data indicate that the rate of MHC synthesis is accelerated in contracting cardiocytes via an increase in translational efficiency. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CARDIOL SECT,CHARLESTON,SC 29401. MED UNIV S CAROLINA,GAZES CARDIAC RES INST,DEPT MED,CHARLESTON,SC 29401. MED UNIV S CAROLINA,GAZES CARDIAC RES INST,DEPT PHYSIOL,CHARLESTON,SC 29401. MED UNIV S CAROLINA,GAZES CARDIAC RES INST,DEPT CELL BIOL & ANAT,CHARLESTON,SC 29401. FU NHLBI NIH HHS [P01 HL48788-01] NR 41 TC 43 Z9 43 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD SEP 15 PY 1995 VL 270 IS 37 BP 21950 EP 21957 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA RU757 UT WOS:A1995RU75700080 PM 7665617 ER PT J AU DANG, H GEISER, AG LETTERIO, JJ NAKABAYASHI, T KONG, LP FERNANDES, G TALAL, N AF DANG, H GEISER, AG LETTERIO, JJ NAKABAYASHI, T KONG, LP FERNANDES, G TALAL, N TI SLE-LIKE AUTOANTIBODIES AND SJOGRENS SYNDROME-LIKE LYMPHOPROLIFERATION IN TGF-BETA KNOCKOUT MICE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID GROWTH-FACTOR-BETA; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; CELL-PROLIFERATION; LYMPHOCYTES-B; FACTOR-BETA-1; EXPRESSION; DISEASE; MOUSE; DEATH AB Mice bearing the TGF-beta 1 null mutation (-/-) develop lymphoid infiltrates in the heart, lungs, salivary glands, and other organs similar to those seen in the pseudolymphoma of Sjogren's Syndrome. We studied sera from -/- mice and found elevated Ab levels to dsDNA, ssDNA, and Sm ribonucleoprotein. No Abs to SSA/Ro or SSB/La and no IgM rheumatoid factor were found. Serum autoantibodies were predominately Ige and were specific as shown by ELISA inhibition studies. Antinuclear Ab patterns on Western blots varied from one mouse to the next, indicating a random process responsible for the diversity. Wild-type and heterozygote mice had no autoantibodies. Ig glomerular deposits were found in -/- mice, indicating that these autoantibodies may be pathogenic. Treatment of -/- mice with dexamethasone or TGF-beta 1 failed to suppress autoantibody production. These mice represent an overlap combining the autoimmune serology of SLE with the tissue infiltrates of SS. Our results support the concept that TGF-beta 1 is an important naturally occurring immunosuppressive cytokine whose absence can lead to a systemic autoimmune disease. C1 NCI,CHEMOPREVENT LAB,BETHESDA,MD 20892. UNIV TEXAS,HLTH SCI CTR,AUDIE L MURPHY MEM VET HOSP,SAN ANTONIO,TX 78284. RP DANG, H (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV CLIN IMMUNOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIA NIH HHS [AG10531]; NIDCR NIH HHS [DE10863, DE09311] NR 32 TC 137 Z9 139 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD SEP 15 PY 1995 VL 155 IS 6 BP 3205 EP 3212 PG 8 WC Immunology SC Immunology GA RU050 UT WOS:A1995RU05000044 PM 7673733 ER PT J AU DERMODY, TS CHAPPELL, JD HOFLER, JG KRAMP, W TYLER, KL AF DERMODY, TS CHAPPELL, JD HOFLER, JG KRAMP, W TYLER, KL TI ERADICATION OF PERSISTENT REOVIRUS INFECTION FROM A B-CELL HYBRIDOMA SO VIROLOGY LA English DT Note ID VIRUS PERSISTENCE; VIRAL-INFECTION; HEMAGGLUTININ; RECEPTORS; TYPE-3; LYMPHOCYTES; SEQUENCE; MURINE; LEADS; RNA AB The 2G10 B-cell hybridoma was found to be persistently infected with reovirus serotype 3 (RV3). The persistently infected 2G10 culture produced approximately 1 X 10(8) plaque-forming units of virus per milliliter of culture lysate, and a majority of cells in the culture were infected, as determined by infectious center assay and immunocytochemistry. Cure of the persistent infection was achieved by passaging 2G10 cells for 33 days (12 passages) in medium containing polyclonal anti-RV3 antiserum and a monoclonal antibody specific for the RV3 attachment protein. After several passages in antibody-free medium, cured 2G10 cells had (1) nondetectable levels of RV3 in cell-culture lysates, (2) no infectious centers per 3 X 10(8) cells, (3) no immunocytochemically detectable RV3 antigen, and (4) no detectable reovirus-specific RNA by reverse transcription-polymerase chain reaction amplification. Additionally, mice inoculated with cured 2G10 cell lysates did not generate antibodies directed against RV3. These observations demonstrate that persistent reovirus infection of a B-cell hybridoma can be cured by passage in medium containing anti-reovirus antibodies and suggest that the maintenance of this persistent infection is dependent on horizontal cell-to-cell transmission of virus in the culture. (C) 1995 Academic Press, Inc. C1 VANDERBILT UNIV,SCH MED,DEPT PEDIAT,NASHVILLE,TN 37232. VANDERBILT UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,NASHVILLE,TN 37232. CENTOCOR INC,DEPT IN VITRO RES & DEV,MALVERN,PA 19355. UNIV COLORADO,HLTH SCI CTR,DEPT NEUROL,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DEPT MED,DENVER,CO 80220. UNIV COLORADO,HLTH SCI CTR,DEPT IMMUNOL MICROBIOL,DENVER,CO 80220. DENVER VET AFFAIRS MED CTR,NEUROL SERV 127,DENVER,CO 80220. RP DERMODY, TS (reprint author), VANDERBILT UNIV,SCH MED,ELIZABETH B LAMB CTR PEDIAT RES,D6227 MCN,NASHVILLE,TN 37232, USA. OI Tyler, Kenneth/0000-0003-3294-5888 FU NIAID NIH HHS [AI32539] NR 28 TC 13 Z9 13 U1 1 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD SEP 10 PY 1995 VL 212 IS 1 BP 272 EP 276 DI 10.1006/viro.1995.1483 PG 5 WC Virology SC Virology GA RV178 UT WOS:A1995RV17800037 PM 7676645 ER PT J AU RAHMAN, MU HUDSON, AP AF RAHMAN, MU HUDSON, AP TI SUBSTRATES FOR YEAST MITOCHONDRIAL CAMP-DEPENDENT PROTEIN-KINASE ACTIVITY SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID SACCHAROMYCES-CEREVISIAE; CYCLIC-AMP; RNA-POLYMERASE; TRANSCRIPTION FACTOR; STRINGENT RESPONSE; BINDING; PHOSPHORYLATION; GENES; ELEMENT; DNA AB We showed that transcription of mitochondrial (mt) genes in Saccharomyces cerevisiae is governed in part by cellular cAMP levels, and that such transcriptional control is mediated via cAMP-dependent protein kinase (cAPK) activity. Here we use in vitro protein kinase assays with intact mitochondria from respiring cells to define protein substrates for mt cAPK. Our data show that there are at least eight mt proteins phosphorylated in a cAMP-dependent manner, ranging in M(r) from 96000 to 9500. Similar assays with organelles from an mtf1 mutant and its wild-type parent strain show no loss of any mt cAPK target proteins, suggesting that Mtf1p (M(r)=40000), the mt RNA polymerase specificity factor, does not require phosphorylation for activity. We further show, using double mutants for TPK1, TPK2, and TPK3, which encode catalytic subunits of the mt cAPY that each of the eight mt substrate proteins is not phosphorylated equivalently by the individual catalytic subunits. (C) 1995 Academic Press, Inc. C1 DEPT VET AFFAIRS MED CTR,MED RES SERV,PHILADELPHIA,PA 19104. HAHNEMANN UNIV,MED COLL PENN,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19104. NR 25 TC 14 Z9 14 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD SEP 5 PY 1995 VL 214 IS 1 BP 188 EP 194 DI 10.1006/bbrc.1995.2273 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA RT426 UT WOS:A1995RT42600026 PM 7669038 ER PT J AU KOSOWER, E AF KOSOWER, E TI IS THERE A GENERALIST LEARNING STYLE SO ACADEMIC MEDICINE LA English DT Letter C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. RP KOSOWER, E (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 3 TC 0 Z9 0 U1 0 U2 1 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD SEP PY 1995 VL 70 IS 9 BP 745 EP 746 DI 10.1097/00001888-199509000-00002 PG 2 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA RU326 UT WOS:A1995RU32600002 PM 7669143 ER PT J AU DROUNGAS, A EHRMAN, RN CHILDRESS, AR OBRIEN, CP AF DROUNGAS, A EHRMAN, RN CHILDRESS, AR OBRIEN, CP TI EFFECT OF SMOKING CUES AND CIGARETTE AVAILABILITY ON CRAVING AND SMOKING-BEHAVIOR SO ADDICTIVE BEHAVIORS LA English DT Article ID CONDITIONED ABSTINENCE; NICOTINE ADDICTION; ABUSE PATIENTS; COCAINE ABUSE; RELAPSE; ALCOHOL; REACTIVITY; EXPOSURE; STIMULI; URGES AB This study examined whether smokers respond differently to smoking cues than to affectively neutral or unpleasant cues without smoking content, and whether reactivity is affected by expectations regarding the opportunity to smoke. Expectancy was manipulated by telling subjects in group SMOKE that they could smoke, and subjects in group NO SMOKE that they could not smoke following each cue-reactivity session. The dependent variables were subjective ratings of ''desire to smoke'', ''high'', ''withdrawal'', and mood, as well as latency to initiate smoking measured in group SMOKE. Statistical analyses demonstrated that only group SMOKE (a) reported greater ''desire to smoke'' and ''withdrawal'' to the smoking cues compared to the baseline, (b) reported greater ''desire to smoke'' to the smoking cues than to the unpleasant or to the neutral cues, and (c) smoked faster after the smoking cues than after the neutral cues. Both groups rated the unpleasant cues as affectively more negative than the neutral cues. The data suggest that the impact of drug-cues on craving and subsequent drug-use is due to their drug-related content, and not to negative affect. Furthermore, the impact of drug-related cues appears to be influenced by perceived drug-availability. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP DROUNGAS, A (reprint author), UNIV PENN,TREATMENT RES CTR,3900 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. FU NIDA NIH HHS [DA3008] NR 51 TC 133 Z9 133 U1 12 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0306-4603 J9 ADDICT BEHAV JI Addict. Behav. PD SEP-OCT PY 1995 VL 20 IS 5 BP 657 EP 673 DI 10.1016/0306-4603(95)00029-C PG 17 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA RW891 UT WOS:A1995RW89100010 PM 8712062 ER PT J AU LU, ES LIN, TS HARMS, BL GAUMNITZ, EA SINGARAM, C AF LU, ES LIN, TS HARMS, BL GAUMNITZ, EA SINGARAM, C TI A SEVERE CASE OF DIVERSION COLITIS WITH LARGE ULCERATIONS SO AMERICAN JOURNAL OF GASTROENTEROLOGY LA English DT Note ID DIFFERENTIAL-DIAGNOSIS; RECTUM; COLON; COLOSTOMY; DISEASE AB Diversion colitis is an inflammatory condition that develops in the excluded segment of colon after surgical diversion. Inflammatory changes include aphthous-type ulcerations, crypt abscesses, and mucosal friability. These features may be seen in other inflammatory diseases of the colon, making diagnosis difficult. Even though symptoms such as cramping pain, bleeding, and mucous discharge have been described with diversion colitis, most cases are asymptomatic. In this article we report a patient who presented with sepsis secondary to severe diversion colitis with several large (2-4 cm in diameter), deep ulcerations and air in the colonic wall, requiring colectomy. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT SURG,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 12 TC 4 Z9 4 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-9270 J9 AM J GASTROENTEROL JI Am. J. Gastroenterol. PD SEP PY 1995 VL 90 IS 9 BP 1508 EP 1510 PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RT775 UT WOS:A1995RT77500028 PM 7661179 ER PT J AU KATO, S SPINALE, FG TANAKA, R JOHNSON, W COOPER, G ZILE, MR AF KATO, S SPINALE, FG TANAKA, R JOHNSON, W COOPER, G ZILE, MR TI INHIBITION OF COLLAGEN CROSS-LINKING - EFFECTS ON FIBRILLAR COLLAGEN AND VENTRICULAR DIASTOLIC FUNCTION SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE DIASTOLE; STIFFNESS ID RAT LEFT-VENTRICLE; HYPERTROPHY; MYOCARDIUM; HYPERTENSION; MUSCLE; DYSFUNCTION; STIFFNESS; FIBROSIS; NETWORK; DISEASE AB The fibrillar collagen network is postulated to be a primary determinant of left ventricular diastolic stiffness. This hypothesis was tested by examining the structural and physiological effects of a reduction in fibrillar collagen content and cross-linking in the intact left ventricle. Collagen cross-linking was inhibited by treating five normal adult pigs with beta-aminopropionitrile (BAPN; 10 g/day po) for 6 wk; five normal untreated pigs served as controls. Left ventricular volume, mass, and function were determined by simultaneous echocardiography and catheterization. Chamber stiffness, defined by pressure vs. volume data, and myocardial stiffness, defined by stress vs. dimension data, were determined from variably loaded beats during dextran infusion. Collagen distribution (% area) and integrity (% confluence) were determined by light microscopy. Collagen content was measured by hydroxyproline assay, and collagen cross-linking was measured by salt extraction. BAPN decreased collagen distribution (% area decreased from 12 +/- 1% in control to 7 +/- 1% in BAPN, P < 0.05), collagen integrity (% confluence decreased from 8 +/- 1% in control to 4 +/- 1% in BAPN, P < 0.05), collagen content (from 36 +/- 2 mg/g dry wt in control to 27 +/- 2 mg/g dry wt in BAPN, P < 0.05), and collagen cross-linking (extractable collagen increased from 21 +/- 2% in control to 28 +/- 2% in BAPN, P < 0.05). BAPN decreased chamber stiffness (0.13 +/- 0.02 in control to 0.06 +/- 0.01 in BAPN, P < 0.05) and myocardial stiffness (10.4 +/- 0.5 in control to 6.6 +/- 0.5 in BAPN, P < 0.05). Thus BAPN inhibited collagen cross-linking, decreased total collagen, and disrupted collagen distribution and integrity. These changes in the fibrillar collagen network resulted in a reduction in chamber and myocardial stiffness. These findings provide evidence to suggest that the fibrillar collagen matrix is a primary determinant of left ventricular diastolic stiffness in the normal heart. C1 MED UNIV S CAROLINA, DEPT MED, DIV CARDIOL, GAZES CARDIAC RES INST, CHARLESTON, SC 29425 USA. MED UNIV S CAROLINA, DEPT SURG, DIV CARDIOTHORAC SURG, CHARLESTON, SC 29425 USA. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, CHARLESTON, SC 29425 USA. FU NHLBI NIH HHS [HL-45024] NR 31 TC 79 Z9 80 U1 0 U2 5 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD SEP PY 1995 VL 269 IS 3 BP H863 EP H868 PG 6 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA RV400 UT WOS:A1995RV40000016 PM 7573529 ER PT J AU HOLLISTER, MS MACK, LA PATTEN, RM WINTER, TC MATSEN, FA VEITH, RR AF HOLLISTER, MS MACK, LA PATTEN, RM WINTER, TC MATSEN, FA VEITH, RR TI ASSOCIATION OF SONOGRAPHICALLY DETECTED SUBACROMIAL/SUBDELTOID BURSAL EFFUSION AND INTRAARTICULAR FLUID WITH ROTATOR CUFF TEAR SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID SHOULDER; TENDON; US AB OBJECTIVE. Although an association between sonographically detected joint fluid and rotator cuff disease has been reported, the significance of sonographically detected subacromial/subdeltoid bursal effusion has not been studied. We examined a group of patients who had shoulder sonography and surgery to determine the association between bursal and joint effusion and surgically proved tears of the rotator cuff. MATERIALS AND METHODS. We retrospectively reviewed the preoperative shoulder sonography reports of 163 patients for the presence of fluid within the subacromial/subdeltoid bursa or glenohumeral joint. Surgical reports were obtained to determine the status of the rotator cuff. The sonographic reports of 232 asymptomatic shoulders were also reviewed to determine the prevalence of fluid within the subacromial/subdeltoid bursa or the glenohumeral joint. RESULTS. Sixty-seven (41%) of the 163 patients had a joint effusion, bursal fluid, or both, Joint effusion alone was seen in 35 patients. Fourteen of these had a normal rotator cuff at surgery, and 21 had a rotator cuff tear (sensitivity, 22%; specificity, 79%; positive predictive value, 60%), Bursal fluid alone was seen in 10 patients, seven of whom had a rotator cuff tear (sensitivity, 7%; specificity, 96%; positive predictive value, 70%), In 22 patients, fluid was seen in both the bursa and the joint; 21 had surgically proved rotator cuff tears (sensitivity, 22%; specificity, 99%; positive predictive value, 95%). Of the 232 asymptomatic shoulders, 16 (6.9%) had isolated joint effusions, eight (3.4%) had isolated bursal effusions, and four (1.7%) had both joint and bursal effusions. CONCLUSION. The sonographic finding of intraarticular fluid alone (without bursal fluid) has both a low sensitivity and a low specificity for the diagnosis of rotator cuff tears. However, the finding of fluid in the subacromial/subdeltoid bursa, especially when combined with a joint effusion, is highly specific and has a high positive predictive value for associated rotator cuff tears, Sonographically detected fluid in both the joint and the bursa is an uncommon finding in asymptomatic shoulders, The sonographic observation of fluid in the subacromial bursa, either isolated or combined with a joint effusion, should prompt a careful evaluation of the supraspinatus tendon for tear. C1 UNIV WASHINGTON,CTR MED,DEPT RADIOL,SEATTLE,WA 98195. UNIV WASHINGTON,MED CTR,DEPT ORTHOPED,SEATTLE,WA 98195. RP HOLLISTER, MS (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT RADIOL,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 15 TC 78 Z9 83 U1 0 U2 2 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD SEP PY 1995 VL 165 IS 3 BP 605 EP 608 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA RQ006 UT WOS:A1995RQ00600021 PM 7645478 ER PT J AU CLEGG, DO REDA, DJ AF CLEGG, DO REDA, DJ TI COMPARISON OF SULFASALAZINE AND PLACEBO FOR THE TREATMENT OF AXIAL, OLIGOARTICULAR AND POLYARTICULAR MANIFESTATIONS OF THE SERONEGATIVE SPONDYLOARTHROPATHIES SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 US DEPT VET AFFAIRS,COOPERAT STUDIES PROGRAM,HINES,IL 60141. US DEPT VET AFFAIRS,COOPERAT STUDIES PROGRAM,SALT LAKE CITY,UT. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1995 VL 38 IS 9 SU S BP 1176 EP 1176 PG 1 WC Rheumatology SC Rheumatology GA RX684 UT WOS:A1995RX68401175 ER PT J AU SCHUMACHER, HR AF SCHUMACHER, HR TI POSSIBLE ROLE OF BONE-MARROW FAT IN THE DEVELOPMENT OF AN INFLAMMATORY RESPONSE TO SICKLED ERYTHROCYTES - REPLY SO ARTHRITIS AND RHEUMATISM LA English DT Letter ID MONOSODIUM URATE CRYSTALS; SYNOVIAL-FLUID C1 UNIV PENN,SCH MED,PHILADELPHIA,PA 19104. RP SCHUMACHER, HR (reprint author), VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104, USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1995 VL 38 IS 9 BP 1349 EP 1350 DI 10.1002/art.1780380939 PG 2 WC Rheumatology SC Rheumatology GA RW603 UT WOS:A1995RW60300038 ER PT J AU NAKABAVASHI, T DANG, H KONG, L GAISER, A LETTERIO, J TALAL, N AF NAKABAVASHI, T DANG, H KONG, L GAISER, A LETTERIO, J TALAL, N TI UP-REGULATION OF CYTOKINE GENE AND ADHESION MOLECULE PROTEIN EXPRESSION IN THE SALIVARY-GLANDS OF TGF-BETA-1 NULL MICE SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NIH,BETHESDA,MD 20892. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1995 VL 38 IS 9 SU S BP 1496 EP 1496 PG 1 WC Rheumatology SC Rheumatology GA RX684 UT WOS:A1995RX68401494 ER PT J AU OGAWA, N DANQ, H KONG, L ANAYA, LM LIU, GT TALAL, N AF OGAWA, N DANQ, H KONG, L ANAYA, LM LIU, GT TALAL, N TI ANALYSIS OF APOPTOSIS-ASSOCIATED GENE-EXPRESSION IN LYMPHOCYTES FROM SJORGENS SYNDROME (SS) PATIENTS SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD SEP PY 1995 VL 38 IS 9 SU S BP 1497 EP 1497 PG 1 WC Rheumatology SC Rheumatology GA RX684 UT WOS:A1995RX68401495 ER PT J AU PLANCHON, SM WUERZBERGER, S FRYDMAN, B WITIAK, DT HUTSON, P CHURCH, DR WILDING, G BOOTHMAN, DA AF PLANCHON, SM WUERZBERGER, S FRYDMAN, B WITIAK, DT HUTSON, P CHURCH, DR WILDING, G BOOTHMAN, DA TI BETA-LAPACHONE-MEDIATED APOPTOSIS IN HUMAN PROMYELOCYTIC LEUKEMIA (HL-60) AND HUMAN PROSTATE-CANCER CELLS - A P53-INDEPENDENT RESPONSE SO CANCER RESEARCH LA English DT Note ID DNA TOPOISOMERASE-I; CAMPTOTHECIN; CARCINOMA; REPAIR; INHIBITOR; CULTURES; BREAKS AB beta-Lapachone and certain of its derivatives directly bind and inhibit topoisomerase I (Topo I) DNA unwinding activity and form DNA-Topo I complexes, which are not resolvable by SDS-K+ assays. We show that beta-lapachone can induce apoptosis in certain cells, such as in human promyelocytic leukemia (HL-60) and human prostate cancer (DU-145, PC-3, acid LNCaP) cells, as also described by Li et al. (Cancer Res., 55: 0000-0000, 1995). Characteristic 180-200-bp oligonucleosome DNA laddering and fragmented DNA-containing apoptotic cells via flow cytometry and morphological examinations were observed in 4 h in HL-60 cells after a 4-h, greater than or equal to 0.5 mu M beta-lapachone exposure. HL-60 cells treated with camptothecin or topotecan resulted in greater apoptotic DNA laddering and apoptotic cell populations than comparable equitoxic concentrations of beta-lapachone, although beta-lapachone was a more effective Topo I inhibitor. beta-Lapachone treatment (4 h, 1-5 mu M) resulted in a block at G(0)/G(1), with decreases in S and G(2)/M phases and increases in apoptotic cell populations over time in HL-60 and three separate human prostate cancer (DU-145, PC-3, and LNCaP) cells. Similar treatments with topotecan or camptothecin (4 h, 1-5 mu M) resulted in blockage of cells in S and apoptosis. Thus, beta-lapachone causes a block in G(0)/G(1) of the cell cycle and induces apoptosis in cells before, or at early times during, DNA synthesis. These events are p53 independent, since PC-3 and HL-60 cells are mil cells, LNCaP are wild-type, and DU-145 contain mutant p53, yet all undergo apoptosis after beta-lapachone treatment. Interestingly, beta-lapachone treatment of p53 wild type-containing prostate cancer cells (i.e., LNCaP) did not result in the induction of nuclear levels of p53 protein, as did camptothecin-treated cells. Like other Topo I inhibitors, beta-lapachone may induce apoptosis by locking Topo I onto DNA, blocking replication fork movement, and inducing apoptosis in a p53-independent fashion. beta-Lapachone and its derivatives, as well as other Topo I inhibitors, have potential clinical utility alone against human leukemia and prostate cancers. C1 UNIV WISCONSIN,SCH MED,DEPT HUMAN ONCOL,MADISON,WI 53792. UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. UNIV WISCONSIN,SCH MED,DIV MED CHEM,MADISON,WI 53792. UNIV WISCONSIN,SCH PHARM,DEPT HUMAN ONCOL,MADISON,WI 53792. UNIV WISCONSIN,SCH PHARM,DEPT MED,MADISON,WI 53792. UNIV WISCONSIN,SCH PHARM,DIV MED CHEM,MADISON,WI 53792. RI Planchon, Sarah/A-5731-2009 FU NCI NIH HHS [R01 CA092250] NR 22 TC 182 Z9 186 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD SEP 1 PY 1995 VL 55 IS 17 BP 3706 EP 3711 PG 6 WC Oncology SC Oncology GA RR739 UT WOS:A1995RR73900006 PM 7641180 ER PT J AU HUANG, M WANG, JY LEE, P SHARMA, S MAO, JT MEISSNER, H UYEMURA, K MODLIN, R WOLLMAN, J DUBINETT, SM AF HUANG, M WANG, JY LEE, P SHARMA, S MAO, JT MEISSNER, H UYEMURA, K MODLIN, R WOLLMAN, J DUBINETT, SM TI HUMAN NON-SMALL-CELL LUNG-CANCER CELLS EXPRESS A TYPE-2 CYTOKINE PATTERN SO CANCER RESEARCH LA English DT Article ID HUMAN-MONOCYTES; IMMUNE-RESPONSE; GENE-EXPRESSION; MESSENGER-RNA; TUMOR-GROWTH; IFN-GAMMA; IN-VITRO; LYMPHOKINE; IL-4; INTERLEUKIN-10 AB In addition to infiltrating inflammatory cells, tumors also produce cytokines and growth factors that may alter tumor growth, tumor immunogenicity, and the host immune response. To characterize the expression profile of human non-small cell lung cancer (NSCLC)-derived cytokines, the mRNA expression of type 1 and type 2 cytokines in five human NSCLC lines was analyzed by reverse transcriptase-PCR. Expression of interleukin 5 (IL-5) and IL-10 was demonstrated in all tumor lines evaluated, whereas IL-4 was present in three of five lines and IL-13 was present in two of five lines. In contrast, none of the tumor lines expressed IL-2 and IFN-gamma. Type 2 cytokine protein production by NSCLC lines was confirmed by immunoprecipitation and cytokine specific ELISA. Tumor-derived IL-10 secretion was significantly augmented by exogenous recombinant cytokines including IL-4 and tumor necrosis factor-alpha. To evaluate whether fresh NSCLC nodules also express a type 2 cytokine pattern, the content of type 1 and type 2 cytokines in tissue homogenates from 13 fresh NSCLC nodules and normal lung surgical specimens was assessed. Human NSCLC nodules contain significantly more type 2 cytokines than does normal lung tissue when corrected for total protein concentration. To identify the cellular source of type 2 cytokine production in tumor nodules, immunohistology was performed on sections from 5 lung squamous cell carcinomas and 5 adenocarcinomas. All of the specimens revealed positive staining for type 2 cytokines within tumor cells. In summary, we report that human NSCLC cells produce type 2 cytokines both in situ and in vitro, which may play an active immunoregulatory role in the lung cancer microenvironment C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET ADM MED CTR,SCH MED,DEPT MED,DIV PULM & CRIT CARE MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,JOHNSON COMPREHENS CANC CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DIV DERMATOL,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90073. VET ADM MED CTR,LOS ANGELES,CA 90073. RI Modlin, Robert/M-7941-2014 OI Modlin, Robert/0000-0003-4720-031X FU NCI NIH HHS [CA09120]; NHLBI NIH HHS [HL07014] NR 66 TC 225 Z9 251 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD SEP 1 PY 1995 VL 55 IS 17 BP 3847 EP 3853 PG 7 WC Oncology SC Oncology GA RR739 UT WOS:A1995RR73900032 PM 7641203 ER PT J AU CASTLE, S WILKINS, S HECK, E TANZY, K FAHEY, J AF CASTLE, S WILKINS, S HECK, E TANZY, K FAHEY, J TI DEPRESSION IN CAREGIVERS OF DEMENTED PATIENTS IS ASSOCIATED WITH ALTERED IMMUNITY - IMPAIRED PROLIFERATIVE CAPACITY, INCREASED CD8(+), AND A DECLINE IN LYMPHOCYTES WITH SURFACE SIGNAL-TRANSDUCTION MOLECULES (CD38(+)) AND A CYTOTOXICITY MARKER (CD56(+) CD8+) SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY LA English DT Article DE DEPRESSION; IMMUNE DYSFUNCTION; CD38; CD56; AGING ID KILLER-CELL-ACTIVITY; STRESS-INDUCED MODULATION; ACTIVATION; DISEASE; SUBSETS; AGE; SUPPRESSION; POPULATIONS; LEUKOCYTES AB Changes in relevant immune parameters, including function, were found to be associated with depression in elderly caregiver wives of demented patients. We studied the relationship between immune cell phenotype and T cell proliferative capacity of such caregivers to levels of stress and depression over the course of a support group intervention. The data indicate the strongest association between depression (of all stress parameters) and impaired T cell proliferative capacity. Depression was also most strongly (of stress parameters) associated with a shift in T cell populations with an increase in CD8(+) T cells, and a reduced percentage of CD38(+) cells in both CD8(+) and CD4(+) T cell populations. Since CD38 is a signal transduction factor, it was interesting that a decreased percentage of CD38(+) cells correlated with impaired T cell function (proliferation). Another significant difference was the reduction in natural killer (NK) cells as well as the percentage of the CD56(+) component of the CD8(+) population. This latter subset is important in MHC-unrestricted cytotoxicity, and has been found expanded in healthy centenarians. This study shows that both chronic stress, and depression in particular, and age have deleterious effects on T cells, and together could significantly contribute to the higher risk of disease and mortality associated with being a caregiver of a demented individual. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,CTR INTERDISCIPLINARY RES & IMMUNOL & DIS,LOS ANGELES,CA. RP CASTLE, S (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN 11G,LOS ANGELES,CA 90073, USA. FU NIA NIH HHS [AG00489-01] NR 42 TC 53 Z9 53 U1 0 U2 5 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0009-9104 J9 CLIN EXP IMMUNOL JI Clin. Exp. Immunol. PD SEP PY 1995 VL 101 IS 3 BP 487 EP 493 PG 7 WC Immunology SC Immunology GA RR203 UT WOS:A1995RR20300017 PM 7545096 ER PT J AU BARCHIESI, F HOLLIS, RJ DELPOETA, M MCGOUGH, DA SCALISE, G RINALDI, MG PFALLER, MA AF BARCHIESI, F HOLLIS, RJ DELPOETA, M MCGOUGH, DA SCALISE, G RINALDI, MG PFALLER, MA TI TRANSMISSION OF FLUCONAZOLE-RESISTANT CANDIDA-ALBICANS BETWEEN PATIENTS WITH AIDS AND OROPHARYNGEAL CANDIDIASIS DOCUMENTED BY PULSED-FIELD GEL-ELECTROPHORESIS SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID GENETIC SIMILARITY; STRAINS; THERAPY AB Electrophoretic karyotype and restriction endonuclease analysis of genomic DNA were used for the typing of nine isolates of Candida albicans from the oral cavities of two patients with AIDS-a husband and wife-whose infections became resistant to treatment with fluconazole (400 mg/d), The in vitro susceptibilities of sequential isolates to fluconazole and two other triazoles, itraconazole and the investigational drug D0870, were also evaluated. DNA analysis showed that the isolates responsible for fluconazole-resistant episodes of oropharyngeal candidiasis in the two patients were genetically related, In vitro susceptibility to fluconazole correlated well with clinical outcome, Although the minimal inhibitory concentrations of itraconazole and D0870 for fluconazole-resistant isolates were higher than those for fluconazole-susceptible isolates, both of the former triazoles exhibited good in vitro activity against the isolates tested. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV IOWA,COLL MED,DEPT PATHOL,CLIN MICROBIOL LAB,IOWA CITY,IA. UNIV ANCONA,IST MALATTIE INFETT & MED PUBL,ANCONA,ITALY. RP BARCHIESI, F (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 25 TC 34 Z9 35 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1995 VL 21 IS 3 BP 561 EP 564 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA RU940 UT WOS:A1995RU94000014 PM 8527544 ER PT J AU RHEW, DC GOETZ, MB LOUIE, MH AF RHEW, DC GOETZ, MB LOUIE, MH TI REVERSIBLE CD4(+) T-LYMPHOCYTE DEPLETION IN A PATIENT WHO HAD DISSEMINATED HISTOPLASMOSIS AND WHO WAS NOT INFECTED WITH HUMAN-IMMUNODEFICIENCY-VIRUS SO CLINICAL INFECTIOUS DISEASES LA English DT Letter ID UNEXPLAINED OPPORTUNISTIC INFECTIONS; HIV-INFECTION; IMMUNOREGULATION C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,DIV INFECT DIS,LOS ANGELES,CA 90073. CEDARS SINAI MED CTR,LOS ANGELES,CA 90048. NR 10 TC 2 Z9 2 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD SEP PY 1995 VL 21 IS 3 BP 702 EP 703 PG 2 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA RU940 UT WOS:A1995RU94000053 PM 8527583 ER PT J AU BAILEY, SR KIESZ, RS AF BAILEY, SR KIESZ, RS TI INTRAVASCULAR STENTS - CURRENT APPLICATIONS - INTRODUCTION SO CURRENT PROBLEMS IN CARDIOLOGY LA English DT Review ID LUMINAL CORONARY ANGIOPLASTY; ANGIOGRAPHIC FOLLOW-UP; SAPHENOUS-VEIN GRAFTS; PALMAZ-SCHATZ STENTS; EXPANDABLE INTRACORONARY STENTS; 1985-1986 NATIONAL-HEART; VENOUS BYPASS GRAFTS; SMOOTH-MUSCLE CELLS; BALLOON ANGIOPLASTY; BLOOD-INSTITUTE C1 AUDIE L MURPHY MEM VET ADM MED CTR,CARDIAC CATHETERIZAT LAB,SAN ANTONIO,TX 78284. RP BAILEY, SR (reprint author), UNIV TEXAS HOSP,CARDIAC CATHETERIZAT LAB,GALVESTON,TX 77550, USA. NR 184 TC 1 Z9 1 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0146-2806 J9 CURR PROB CARDIOLOGY JI Curr. Probl. Cardiol. PD SEP PY 1995 VL 20 IS 9 BP 618 EP & PG 0 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA RV228 UT WOS:A1995RV22800002 ER PT J AU MATSUYAMA, SS YAMAGUCHI, DT VERGARA, J JARVIK, LF AF MATSUYAMA, SS YAMAGUCHI, DT VERGARA, J JARVIK, LF TI TETRAETHYLAMMONIUM-INDUCED CALCIUM-CONCENTRATION CHANGES IN SKIN FIBROBLASTS FROM PATIENTS WITH ALZHEIMER-DISEASE SO DEMENTIA LA English DT Article DE ALZHEIMER DISEASE; POTASSIUM CHANNEL; FIBROBLASTS; CALCIUM; DIAGNOSIS ID POTASSIUM-CHANNEL BLOCKERS; OSTEOBLAST-LIKE CELLS; WORK AB Potassium (Kc) channel dysfunction in fibroblasts was recently proposed as a potential diagnostic marker for Alzheimer disease (AD), We utilized a microspectrofluorometric method with Fura-2AM to measure intracellular free calcium ([Ca2+](i)) following depolarization with the K+ channel blocker tetraethylammonium (TEA) in seven AD and seven control fibroblast cultures, Contrary to our expectation, 43% of the AD and 36% of the control fibroblast plated coverglasses responded with an increase in [Ca2+](i) on addition of 100 mM TEA. The data suggest that the TEA-elicited [Ca2+](i) response is not a useful AD screening test. C1 UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PHYSIOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,INST NEUROPSYCHIAT,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP MATSUYAMA, SS (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,PSYCHOGERIATR LAB B151R,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. FU NIA NIH HHS [P30 AG10123] NR 11 TC 11 Z9 11 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1013-7424 J9 DEMENTIA JI Dementia PD SEP-OCT PY 1995 VL 6 IS 5 BP 241 EP 244 DI 10.1159/000106953 PG 4 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA RU300 UT WOS:A1995RU30000001 PM 8528369 ER PT J AU KLEIN, RL LAIMINS, M LOPESVIRELLA, MF AF KLEIN, RL LAIMINS, M LOPESVIRELLA, MF TI ISOLATION, CHARACTERIZATION, AND METABOLISM OF THE GLYCATED AND NONGLYCATED SUBFRACTIONS OF LOW-DENSITY LIPOPROTEINS ISOLATED FROM TYPE-I DIABETIC-PATIENTS AND NONDIABETIC SUBJECTS SO DIABETES LA English DT Article ID MONOCYTE-DERIVED MACROPHAGES; OXIDATIVE MODIFICATION; INVITRO OXIDATION; ENHANCED SUSCEPTIBILITY; BEZAFIBRATE THERAPY; GLYCOSYLATION; NORMALIZATION; IDENTIFICATION; HETEROGENEITY; FIBROBLASTS AB The total low-density lipoprotein (LDL) fraction was isolated from 21 patients with type I diabetes and 7 nondiabetic normolipemic subjects. The LDL was separated into two subfractions, one glycated (G-LDL) and one nonglycated (N-LDL), using affinity chromatography. G-LDL comprised 21.1 +/- 3.6 and 5.2 +/- 0.6% of the total LDL in diabetic patients and normal subjects, respectively. G-LDL isolated from both diabetic patients and normal subjects was significantly more glycated than N-LDL isolated from the same subject. G-LDL isolated from both diabetic patients and normal subjects was enriched in triglycerides. The metabolism of N-LDL and G-LDL was investigated in human fibroblasts, which express only the classical LDL receptor, and in human monocyte-derived macrophages, which also express a receptor for G-LDL. In fibroblasts, the rates of receptor-mediated accumulation of N-LDL isolated from normal subjects and diabetic patients were significantly greater (P < 0.01) than those of G-LDL. In contrast, when the same LDL subfractions were incubated with human monocyte-derived macrophages, the rates of receptor-mediated accumulation of G-LDL isolated from both groups were significantly greater (P < 0.01) than those of N-LDL. Rates of degradation of G-LDL by human macrophages were not significantly different hom those of N-LDL during short-term incubations but reached statistical significance (P < 0.05) when LDL subfractions were incubated with cells for 24 h. G-LDL stimulated cholesteryl ester synthesis rates in human macrophages significantly (P < 0.05) more than N-LDL from the same subject and thus may contribute to the increased prevalence of atherosclerosis in diabetic patients. RP KLEIN, RL (reprint author), RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,RES SERV,109 BEE ST,CHARLESTON,SC 29401, USA. NR 42 TC 79 Z9 80 U1 1 U2 2 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD SEP PY 1995 VL 44 IS 9 BP 1093 EP 1098 DI 10.2337/diabetes.44.9.1093 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TD623 UT WOS:A1995TD62300014 PM 7657034 ER PT J AU ROSENBEK, JC AF ROSENBEK, JC TI EFFICACY IN DYSPHAGIA SO DYSPHAGIA LA English DT Article; Proceedings Paper CT 3rd Annual Meeting of the Dysphagia-Research-Society CY OCT 14-16, 1994 CL MCLEAN, VA SP Dysphagia Res Soc DE DYSPHAGIA; TREATMENT; EFFICACY; SWALLOWING THERAPY; DEGLUTITION; DEGLUTITION DISORDERS AB Data demonstrating the efficacy of behavioral methods for treating dysphagia are in short supply. This paper defines efficacy and distinguishes it from efficiency. It highlights the attitudes,measures, decisions, and data essential to the design of clinical trials. The need for additional outcome measures and for establishing appropriate treatment intensities are emphasized. RP ROSENBEK, JC (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT SPEECH PATHOL & AUDIOL,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 22 TC 14 Z9 14 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0179-051X J9 DYSPHAGIA JI Dysphagia PD FAL PY 1995 VL 10 IS 4 BP 263 EP 267 DI 10.1007/BF00431420 PG 5 WC Otorhinolaryngology SC Otorhinolaryngology GA RZ393 UT WOS:A1995RZ39300009 PM 7493508 ER PT J AU GAUMNITZ, EA BASS, P OSINSKI, MA SWEET, MA SINGARAM, C AF GAUMNITZ, EA BASS, P OSINSKI, MA SWEET, MA SINGARAM, C TI ELECTROPHYSIOLOGICAL AND PHARMACOLOGICAL RESPONSES OF CHRONICALLY DENERVATED LOWER ESOPHAGEAL SPHINCTER OF THE OPOSSUM SO GASTROENTEROLOGY LA English DT Article ID NITRIC-OXIDE SYNTHASE; SMOOTH-MUSCLE; RAT JEJUNUM; ACHALASIA; JUNCTION; TRANSMITTER; MEDIATOR; PRESSURE; NEURONS; INVIVO AB Background and Aims: Achalasia is characterized by loss of myenteric neurons and incomplete relaxation of the lower esophageal sphincter (LES). The aim of this study was to develop an achalasia model in the opossum using the surfactant benzyldimethyltetradecylammonium chloride (BAC). This study further characterizes the achalasia model. Methods: BAC or saline was injected circumferentially into the LES of 14 adult opossums. Eight months after injection, manometry, isolated muscle bath studies, electrical field stimulation, and histochemical analysis were performed. Results: Manometrically, the LES of BAC-treated opossums showed higher pressures (38.7 +/- 12 mm Hg vs. 17 +/- 3.0 mm Hg) and reduced esophageal body contraction amplitudes (4.2 +/- 3 mm Hg vs. 27.4 +/- 12 mm Hg). Isolated muscle strips challenged with carbachol and sodium nitroprusside contracted and relaxed similarly to controls. Electrical field stimulation failed to induce relaxation in BAC-treated tissue but did induce contraction. Contractile responses were markedly reduced by tetrodotoxin and atropine in BAC-treated animals and controls. An altered nitric oxide system was shown by the lack of response to L-arginine and N-omega-nitro-L-arginine. Histology showed loss of myenteric neurons and increased cholinergic nerve bundles. Conclusions: Loss of NO inhibitory myenteric neurons markedly reduces the relaxation of the LES, and histology and pharmacological responses suggest a proliferation of cholinergic nerves into the LES contributing to the static elevated pressures of the amyenteric LES. C1 UNIV WISCONSIN,DEPT MED,DIV GASTROENTEROL,MADISON,WI 53792. UNIV WISCONSIN,SCH PHARM,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. NR 38 TC 21 Z9 24 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD SEP PY 1995 VL 109 IS 3 BP 789 EP 799 DI 10.1016/0016-5085(95)90386-0 PG 11 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RT115 UT WOS:A1995RT11500014 PM 7657107 ER PT J AU MARRA, F GRANDALIANO, G VALENTE, AJ ABBOUD, HE AF MARRA, F GRANDALIANO, G VALENTE, AJ ABBOUD, HE TI THROMBIN STIMULATES PROLIFERATION OF LIVER FAT-STORING CELLS AND EXPRESSION OF MONOCYTE CHEMOTACTIC PROTEIN-1 - POTENTIAL ROLE IN LIVER-INJURY SO HEPATOLOGY LA English DT Article ID RAT HEPATIC LIPOCYTES; GROWTH-FACTOR; INTRAVASCULAR COAGULATION; CONDITIONED MEDIUM; BLOOD-COAGULATION; MATRIX SYNTHESIS; ALPHA-THROMBIN; ACTIVATION; RECEPTOR; FIBROSIS AB Liver fat-storing cells (FSC) proliferate and secrete extracellular matrix in experimental models of liver injury. In this study, we determined if thrombin, a serine protease produced during acute and chronic tissue injury, modulates the functions of FSC. Thrombin stimulated DNA synthesis and proliferation of FSC, as assessed by [H-3]-thymidine incorporation assay and measurement of cell number, respectively. Thrombin also increased the secretion of monocyte chemotactic protein-1 (MCP-1) in a time- and dose-dependent fashion. The effect of thrombin on both DNA synthesis and MCP-1 secretion was neutralized by pretreatment of thrombin with hirudin. The increased MCP-1 secretion was associated with increased steady-state levels of MCP-1 messenger RNA. Pretreatment of FSC with 5 mu mol/L retinol for 48 hours inhibited the mitogenic effects of thrombin but not the induction of MCP-1 secretion. FSC express specific transcripts encoding for the human thrombin receptor, as shown by Northern blot analysis of poly(A)(+) RNA. Proteolytic activation of the thrombin receptor results in the formation of a new N-terminus that functions as a tethered ligand. We studied the effects of a thrombin receptor activating peptide (TRAP) corresponding to the newly formed N-terminus, on FSC. TRAP mimicked the effects of thrombin on [H-3]thymidine incorporation, MCP-1 secretion, and MCP-1 gene expression. This study suggests that thrombin may be involved in modulating FSC proliferation and monocyte chemotaxis during human liver disease, through proteolytic activation of its receptor. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. RI Grandaliano, Giuseppe/G-2963-2012; Marra, Fabio/K-7263-2016 OI Marra, Fabio/0000-0001-8629-0878 FU NHLBI NIH HHS [HL-26890]; NIDDK NIH HHS [DK-33665, DK-43988] NR 47 TC 89 Z9 91 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD SEP PY 1995 VL 22 IS 3 BP 780 EP 787 DI 10.1016/0270-9139(95)90297-X PG 8 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RT116 UT WOS:A1995RT11600013 PM 7657283 ER PT J AU SERRATOSA, JM DELGADOESCUETA, AV POSADA, I SHIH, S DRURY, I BERCIANO, J ZABALA, JA ANTUNEZ, MC SPARKES, RS AF SERRATOSA, JM DELGADOESCUETA, AV POSADA, I SHIH, S DRURY, I BERCIANO, J ZABALA, JA ANTUNEZ, MC SPARKES, RS TI THE GENE FOR PROGRESSIVE MYOCLONUS EPILEPSY OF THE LAFORA TYPE MAPS TO CHROMOSOME 6Q SO HUMAN MOLECULAR GENETICS LA English DT Article ID LINKAGE ANALYSIS; SKIN BIOPSY; DIAGNOSIS; DISEASE; DNA AB Progressive myoclonus epilepsy of the Lafora type (Lafora's disease) is an autosomal recessive disease characterized by epilepsy, myoclonus, dementia, and periodic acid-Schiff-positive intracellular inclusion bodies. The inclusion deposits consist of branched polysaccharides (polyglucosans) but the responsible biochemical defect has not been identified. Onset is during late childhood or adolescence and the disease leads to a fatal outcome within a decade of first symptoms. We studied nine families in which Lafora's disease had been proven by biopsy in at least one member. In order to locate the responsible gene, we screened the human genome with microsatellite markers spaced an average of 13 cM. We used linkage analysis in all nine families and homozygosity mapping in four consanguineous families to define the Lafora's disease gene region. Two point linkage analysis resulted in a total peak lod score of 10.54 for marker D6S311. Six additional chromosome 6q23-25 microsatellites yielded lod scores ranging from 5.92 to 9.60 at theta(m=f) = 0. An extended pedigree with five affected members independently proved linkage with peak lod scores over 3.8 at theta(m=f) = 0 for D6S292, D6S403, and D6S311. The multipoint one-led-unit support interval covered a 2.5 cM region surrounding D6S403. Homozygosity mapping defined a 17 cM region in chromosome 6q23-25 flanked by D6S292 and D6S420 that contains the Lafora's disease gene. C1 UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. HOSP DOCE OCTUBRE,DEPT NEUROL,E-28041 MADRID,SPAIN. UNIV MICHIGAN HOSP,DEPT NEUROL,ANN ARBOR,MI 48109. UNIV CANTABRIA,HOSP MARQUES VALDECILLA,DEPT NEUROL,E-39008 SANTANDER,SPAIN. CLIN PUERTA HIERRO,NEUROL SERV,E-28035 MADRID,SPAIN. HOSP UNIV VIRGEN ARRIXACA,NEUROL SERV,E-30120 MURCIA,SPAIN. UNIV CALIF LOS ANGELES,DEPT MED,DIV MED GENET,LOS ANGELES,CA 90024. RP SERRATOSA, JM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CALIF COMPREHENS EPILEPSY PROGRAM,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. FU NINDS NIH HHS [5P01-NS21908] NR 36 TC 93 Z9 93 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0964-6906 J9 HUM MOL GENET JI Hum. Mol. Genet. PD SEP PY 1995 VL 4 IS 9 BP 1657 EP 1663 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA RT124 UT WOS:A1995RT12400029 PM 8541857 ER PT J AU FRAIMOW, HS ABRUTYN, E AF FRAIMOW, HS ABRUTYN, E TI PATHOGENS RESISTANT TO ANTIMICROBIAL AGENTS - EPIDEMIOLOGY, MOLECULAR MECHANISMS, AND CLINICAL MANAGEMENT SO INFECTIOUS DISEASE CLINICS OF NORTH AMERICA LA English DT Review ID SPECTRUM BETA-LACTAMASES; HIGH-LEVEL RESISTANCE; PENICILLIN-BINDING PROTEINS; STAPHYLOCOCCUS-AUREUS; ENTEROCOCCUS-FAECIUM; STREPTOCOCCUS-PNEUMONIAE; PNEUMOCOCCAL MENINGITIS; VANCOMYCIN-RESISTANT; ESCHERICHIA-COLI; HAEMOPHILUS-INFLUENZAE AB Over the last decade, an ominous trend in the field of antimicrobial chemotherapy has been the increasing pace of the development of antimicrobial resistance in bacterial pathogens. This article reviews the strategies used by bacteria to avoid the action of antimicrobial agents and how these resistance traits are disseminated. Resistance among the common community and nosocomially acquired pathogens, and the current approach to therapy of these organisms are discussed in this article. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. GRAD HOSP PHILADELPHIA,PHILADELPHIA,PA 19104. MED COLL PENN,PHILADELPHIA,PA 19104. HAHNEMANN UNIV,PHILADELPHIA,PA 19104. NR 113 TC 31 Z9 31 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0891-5520 J9 INFECT DIS CLIN N AM JI Infect. Dis. Clin. North Am. PD SEP PY 1995 VL 9 IS 3 BP 497 EP & PG 0 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA RV007 UT WOS:A1995RV00700004 PM 7490430 ER PT J AU BUSH, LM CALMON, J JOHNSON, CC AF BUSH, LM CALMON, J JOHNSON, CC TI NEWER PENICILLINS AND BETA-LACTAMASE INHIBITORS SO INFECTIOUS DISEASE CLINICS OF NORTH AMERICA LA English DT Review ID PLUS CLAVULANIC ACID; GRANULOCYTOPENIC CANCER-PATIENTS; BACTEROIDES-FRAGILIS GROUP; PIPERACILLIN-TAZOBACTAM; PSEUDOMONAS-AERUGINOSA; BINDING PROTEINS; INVITRO ACTIVITY; ESCHERICHIA-COLI; AMPICILLIN-SULBACTAM; ANAEROBIC-BACTERIA AB Penicillins continue to be essential antibiotics for the treatment and prophylaxis of many infectious diseases. Recent advances have resulted in compounds with favorable new antimicrobial and pharmacologic properties. This article reviews the spectrum of activity, toxicity, pharmacokinetics, and clinical uses of the extended spectrum penicillins and the beta-lactamase inhibitor combination agents. C1 MED COLL PENN,DIV INFECT DIS,PHILADELPHIA,PA 19129. HAHNEMANN UNIV,SCH MED,PHILADELPHIA,PA 19129. JFK MED CTR,W PALM BEACH,FL. CROZER CHESTER MED CTR,CHESTER,PA. VET AFFAIRS MED CTR,PHILADELPHIA,PA. NR 154 TC 13 Z9 13 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0891-5520 J9 INFECT DIS CLIN N AM JI Infect. Dis. Clin. North Am. PD SEP PY 1995 VL 9 IS 3 BP 653 EP & PG 0 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA RV007 UT WOS:A1995RV00700012 PM 7490438 ER PT J AU LANDAU, WM DAUBE, JR AMINOFF, MJ BREY, RL BROOKS, BR DEUEL, RK GALABURDA, AM PORTER, JA ROSENBAUM, RB WHITHAM, RH WIGGS, JW AF LANDAU, WM DAUBE, JR AMINOFF, MJ BREY, RL BROOKS, BR DEUEL, RK GALABURDA, AM PORTER, JA ROSENBAUM, RB WHITHAM, RH WIGGS, JW TI NEUROREALITY .1. DEDICATED DEMOLITION OF THE DECADE OF THE BRAIN - THE GENUINE THREAT TO NEUROLOGIC RESEARCH FROM THE ANIMAL RADICAL RIGHT SO JOURNAL OF CHILD NEUROLOGY LA English DT Editorial Material C1 WASHINGTON UNIV,SCH MED,DEPT NEUROL,ST LOUIS,MO 63110. WASHINGTON UNIV,SCH MED,DEPT PEDIAT,ST LOUIS,MO 63110. MAYO CLIN,DEPT NEUROL,ROCHESTER,MN. UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143. UNIV TEXAS,HLTH SCI CTR,DEPT MED NEUROL,SAN ANTONIO,TX 78285. WILLIAM S MIDDLETON MEM VET ADM MED CTR,NEUROL SERV,MADISON,WI. BETH ISRAEL HOSP,BEHAV NEUROL UNIT,BOSTON,MA 02215. OREGON HLTH SCI UNIV,DEPT NEUROL,PORTLAND,OR 97201. NR 0 TC 2 Z9 2 U1 0 U2 0 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON ST, PO BOX 620, LCD 1, HAMILTON ON L8N 3K7, CANADA SN 0883-0738 J9 J CHILD NEUROL JI J. Child Neurol. PD SEP PY 1995 VL 10 IS 5 BP 343 EP 345 PG 3 WC Clinical Neurology; Pediatrics SC Neurosciences & Neurology; Pediatrics GA RV170 UT WOS:A1995RV17000001 PM 7499752 ER PT J AU LEONG, GB SILVA, JA AF LEONG, GB SILVA, JA TI PSYCHIATRIC-LEGAL ANALYSIS OF CRIMINAL DEFENDANTS CHARGED WITH MURDER - A SAMPLE WITHOUT MAJOR MENTAL DISORDER SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE PSYCHIATRY; HOMICIDE; MURDER; SUBSTANCE ABUSE; VIOLENCE; AGGRESSION; CULTURAL FACTORS AB A sample of 31 criminal defendants who were charged with murder but without major mental disorder was studied. The sample was derived from a large urban multi-ethnic, multi-cultural community pool. Subject characteristics and information about the homicide and decedents are described. Suggestions for further study are briefly discussed. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 26 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD SEP PY 1995 VL 40 IS 5 BP 858 EP 861 PG 4 WC Medicine, Legal SC Legal Medicine GA RX718 UT WOS:A1995RX71800031 PM 7595330 ER PT J AU LEONG, GB SILVA, JA WEINSTOCK, R AF LEONG, GB SILVA, JA WEINSTOCK, R TI ANOTHER COURTROOM ASSAULT ON THE CONFIDENTIALITY OF THE PSYCHOTHERAPIST-PATIENT RELATIONSHIP SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE PSYCHIATRY; PSYCHOTHERAPY; PRIVILEGE; CONFIDENTIALITY; PRIVACY AB The therapeutic and legal protections afforded by California's psychotherapist-patient privilege have become increasingly eroded in such recent cases as People v. Wharton and Menendez v. Superior Court. In another capital case, People v. Webb, the California Supreme Court further erodes this privilege in regard to the private (confidential) treatment records of a prosecution witness. The Webb case and its possible implications are explored. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 7 TC 2 Z9 2 U1 0 U2 0 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD SEP PY 1995 VL 40 IS 5 BP 862 EP 864 PG 3 WC Medicine, Legal SC Legal Medicine GA RX718 UT WOS:A1995RX71800032 PM 7595331 ER PT J AU VESCIO, RA CAO, J HONG, CH LEE, JC WU, CH DANIELIAN, MD WU, V NEWMAN, R LICHTENSTEIN, AK BERENSON, JR AF VESCIO, RA CAO, J HONG, CH LEE, JC WU, CH DANIELIAN, MD WU, V NEWMAN, R LICHTENSTEIN, AK BERENSON, JR TI MYELOMA IG HEAVY-CHAIN-V REGION SEQUENCES REVEAL PRIOR ANTIGENIC SELECTION AND MARKED SOMATIC MUTATION BUT NO INTRACLONAL DIVERSITY SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MALIGNANT PLASMA-CELL; MULTIPLE-MYELOMA; BONE-MARROW; GERMINAL-CENTERS; IMMUNE-RESPONSE; ANTIBODY DIVERSITY; CLONAL EXPANSION; B-CELLS; GENE; REPERTOIRE AB The Ig V-H region sequence in 48 patients with multiple myeloma (MM) was analyzed to characterize the malignant cell of origin. The sequences were obtained after amplification of bone marrow cDNA by using V-H family-specific and C-H primers, then compared with either directly sequenced patient germ-line or published V, gene sequences to assay for somatic mutation. Because somatic hypermutation of the V, gene occurs late in B cell development, its presence has been helpful in determining the cell of origin in other B cell malignancies. Overall, a median of 8.2% of the nucleotides had evidence of substitution within each V-H gene sequence (range = 2.7% to 16.5%), which is more prevalent than in any other reported tumor type. Strong evidence of prior antigenic selection pressure was also evident. The ratio of nucleotide substitutions that resulted in amino acid replacement was significantly higher in the complementarity-determining region than in the framework region (3.25 vs 1.56, respectively; p < 0.00005). No V-H gene intraclonal diversity was noted, despite sequencing multiple clones (3-16) from each patient, nor was there evidence of further VH gene somatic mutation over the course of three patients' disease. These findings strongly imply that the malignant clone in MM evolves from a cell late in B cell development. C1 W LOS ANGELES DEPT VET AFFAIRS MED CTR,DIV GASTROENTEROL,LOS ANGELES,CA 90073. IDEC PHARMACEUT CORP,SAN DIEGO,CA 92121. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,DIV HEMATOL ONCOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90024. RP VESCIO, RA (reprint author), W LOS ANGELES DEPT VET AFFAIRS MED CTR,DIV HEMATOL ONCOL,BLDG 500,ROOM 4237 111H,LOS ANGELES,CA 90073, USA. NR 62 TC 115 Z9 116 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD SEP 1 PY 1995 VL 155 IS 5 BP 2487 EP 2497 PG 11 WC Immunology SC Immunology GA RP743 UT WOS:A1995RP74300022 PM 7650379 ER PT J AU OGAWA, N DANG, H LAZARIDIS, K MCGUFF, HS AUFDEMORTE, TB TALAL, N AF OGAWA, N DANG, H LAZARIDIS, K MCGUFF, HS AUFDEMORTE, TB TALAL, N TI ANALYSIS OF TRANSFORMING GROWTH-FACTOR-BETA AND OTHER CYTOKINES IN AUTOIMMUNE EXOCRINOPATHY (SJOGRENS-SYNDROME) SO JOURNAL OF INTERFERON AND CYTOKINE RESEARCH LA English DT Article ID MYELIN BASIC-PROTEIN; EPSTEIN-BARR VIRUS; II COLLAGEN; MONOCLONAL-ANTIBODIES; RHEUMATOID-ARTHRITIS; ORAL TOLERIZATION; IMMUNE-RESPONSES; LYMPHOID-CELLS; EXPRESSION; SUPPRESSION AB Cytokines play a major role in tissue destruction caused by autoimmune dysregulation, In Sjogren's syndrome (SS) patients, salivary glands are the target organs for autoimmune tissue damage, In the present study, reverse trancriptase-polymerase chain reaction (RT-PCR) was used to look for cytokine mRNA expressed in SS salivary glands, Focus score was used to determine the severity of the lesions, Cytokine production in supernatants of the salivary gland cell culture was measured by enzyme-linked immunosorbent assay (ELISA), Immunohistochemical staining was used to identify the local presence of transforming growth factor beta (TGF-beta), Interleukin (IL)-2, IL-6, and IL-10 mRNA were expressed in moderate to severe SS salivary gland lesions, TGF-P mRNA was constitutively expressed in normal and SS salivary glands, In SS salivary gland cell cultures, IL-6 and IL-10 proteins were produced, TGF-beta production was reduced in high focus score SS glands, Normal and minimally involved SS salivary gland ductal epithelium and acinar cells were found to produce TGF-beta by immunostaining, In conclusion, an excess production of IL-2, IL-6, and IL-10 and a reduced production of the immunosuppressive cytokine, TGF-beta, may be responsible for the progression of the salivary gland lesion in SS, Specific immunotherapy can now be designed based on mechanisms to correct this cytokine imbalance and benefit patients with autoimmune diseases, such as SS. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MICROBIOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP OGAWA, N (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIDCR NIH HHS [DE10863, DE09311] NR 57 TC 39 Z9 39 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1079-9907 J9 J INTERF CYTOK RES JI J. Interferon Cytokine Res. PD SEP PY 1995 VL 15 IS 9 BP 759 EP 767 DI 10.1089/jir.1995.15.759 PG 9 WC Biochemistry & Molecular Biology; Cell Biology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Immunology GA RW068 UT WOS:A1995RW06800002 PM 8536103 ER PT J AU NEUMAR, RW DEGRACIA, DJ WHITE, BC MCDERMOTT, PJ EVANS, DR KRAUSE, GS AF NEUMAR, RW DEGRACIA, DJ WHITE, BC MCDERMOTT, PJ EVANS, DR KRAUSE, GS TI EUKARYOTIC INITIATION-FACTOR 4E DEGRADATION DURING BRAIN ISCHEMIA SO JOURNAL OF NEUROCHEMISTRY LA English DT Note DE CEREBRAL ISCHEMIA; PROTEIN DEGRADATION; CALPAIN; TRANSLATION INITIATION FACTOR ID CAP-BINDING-PROTEIN; TRANSIENT FOREBRAIN ISCHEMIA; DELAYED NEURONAL DEATH; MESSENGER-RNA; HEAT-SHOCK; RAT-BRAIN; CEREBRAL-ISCHEMIA; HIPPOCAMPUS; PHOSPHORYLATION; TRANSLATION AB Suppression of protein synthesis in the brain following an ischemic insult has been thought to occur because of inhibition of translation initiation. All eukaryotic mRNAs, with the exception of heat-shock transcripts, require the activity of eukaryotic initiation factor (eIf) 4E for formation of the translation initiation complex, and eIF-4E availability is rate-limiting. The response of brain eIF-4E concentration and phosphorylation following decapitation ischemia was studied in rat brain homogenates after electrophoresis and western blotting with antibodies against eIF-4E and phosphoserine, respectively. There was no change in level of eIF-4E after 5 min of ischemia (p = 0.82 vs. time 0), but it had decreased 32 (p = 0.01) and 57% (p = 0.006) after 10 and 20 min of ischemia, respectively. There was no loss of serine phosphorylation on eIF-4E beyond signal loss observed due to degradation of the protein itself (p = 0.31). In vitro exposure of eIF-4E to activated mu-calpain resulted in a 50% loss in 10 min of eIF-4E on western blots. If active eIF-4E is required for translation of its own mRNA, degradation of this protein during ischemia, possibly by activated mu-calpain, could be a direct mechanism of irreversible neuronal injury, and the rate of proteolysis of eIF-4E could place an upper time limit on the maximal duration of global brain ischemia compatible with neurologic recovery. C1 WAYNE STATE UNIV,SCH MED,DEPT EMERGENCY MED,DETROIT,MI 48201. WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM,DETROIT,MI 48201. MED UNIV S CAROLINA,DEPT MED,DIV CARDIOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,GAZES CARDIAC RES INST,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. NR 34 TC 26 Z9 27 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD SEP PY 1995 VL 65 IS 3 BP 1391 EP 1394 PG 4 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA RQ571 UT WOS:A1995RQ57100053 PM 7643117 ER PT J AU PAHL, JJ MAZZIOTTA, JC BARTZOKIS, G CUMMINGS, J ALTSCHULER, L MINTZ, J MARDER, SR PHELPS, ME AF PAHL, JJ MAZZIOTTA, JC BARTZOKIS, G CUMMINGS, J ALTSCHULER, L MINTZ, J MARDER, SR PHELPS, ME TI POSITRON-EMISSION TOMOGRAPHY IN TARDIVE-DYSKINESIA SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID CEREBRAL GLUCOSE-UTILIZATION; SUPERIOR CERVICAL-GANGLION; COMPUTED-TOMOGRAPHY; ELECTRICAL-STIMULATION; HUNTINGTONS-DISEASE; BASAL GANGLIA; SCHIZOPHRENIA; METABOLISM; RAT; ACTIVATION AB Cerebral glucose metabolic rates were determined in normal control subjects (n = 26) and schizophrenic patients with tardive dyskinesia (n = 14). Globus pallidus and primary motor cortex (precentral gyrus) metabolic values divided by those of the cerebral hemispheres were significantly increased in the patient group. A similar metabolic pattern has not been reported for schizophrenic patients without tardive dyskinesia, and the abnormalities were demonstrated despite the normal appearance of the basal ganglia on X-ray CT. The findings differed markedly from the reduced metabolic rates of the basal ganglia previously identified in other choreiform disorders, including Huntington's and Wilson's diseases. The findings suggest that tardive dyskinesia is characterized by increased pallidal synaptic activity resulting from either altered striatopallidal input or increased pallidal interneuron firing. C1 UNIV OKLAHOMA,HLTH SCI CTR,DEPT PSYCHIAT,OKLAHOMA CITY,OK 73190. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,NUCL MED LAB,LOS ANGELES,CA 90024. RI Bartzokis, George/K-2409-2013 FU NIMH NIH HHS [R01 MH3791-AC03-76-SF0012]; NINDS NIH HHS [P01 NS15654] NR 60 TC 12 Z9 13 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD FAL PY 1995 VL 7 IS 4 BP 457 EP 465 PG 9 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA TC116 UT WOS:A1995TC11600003 PM 8555748 ER PT J AU SULTZER, DL MAHLER, ME MANDELKERN, MA CUMMINGS, JL VANGORP, WG HINKIN, CH BERISFORD, MA AF SULTZER, DL MAHLER, ME MANDELKERN, MA CUMMINGS, JL VANGORP, WG HINKIN, CH BERISFORD, MA TI THE RELATIONSHIP BETWEEN PSYCHIATRIC-SYMPTOMS AND REGIONAL CORTICAL METABOLISM IN ALZHEIMERS-DISEASE SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID POSITRON EMISSION TOMOGRAPHY; FRONTAL-LOBE; GLUCOSE-METABOLISM; PRIMARY DEMENTIA; MAJOR DEPRESSION; RATING-SCALE; DELUSIONS; BEHAVIOR; HYPOMETABOLISM; PSYCHOSIS AB Cognitive and noncognitive psychiatric symptoms we-re systematically evaluated in 21 patients with Alzheimer's disease by using the Neurobehavioral Rating Scale. Regional cerebral metabolic activity was measured in each patient by [F-18]fluorodeoxyglucose PET. Significant correlations emerged between global cortical metabolic activity and the Agitation/Disinhibition factor score, Cognition factor scare, and total score. Relationships between noncognitive symptoms and metabolic activity were regionally specific, with significant correlations between Agitation/Disinhibition factor score and metabolism in the frontal and temporal lobes, between Psychosis factor score and metabolism in the frontal lobe, and between Anxiety/Depression factor score and metabolism in the parietal lobe. These results suggest that psychiatric symptoms are fundamental expressions of the cortical dysfunction of Alzheimer's disease. C1 W LOS ANGELES VET AFFAIRS MED CTR,NUCL MED SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. RP SULTZER, DL (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,11301 WILSHIRE BLVD,BLDG 256,LOS ANGELES,CA 90073, USA. FU NIA NIH HHS [AG10123]; NIMH NIH HHS [MH00910] NR 66 TC 142 Z9 142 U1 3 U2 6 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD FAL PY 1995 VL 7 IS 4 BP 476 EP 484 PG 9 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA TC116 UT WOS:A1995TC11600006 PM 8555751 ER PT J AU DCRUZ, DP WISNIESKI, JJ ASHERSON, RA KHAMASHTA, MA HUGHES, GRV AF DCRUZ, DP WISNIESKI, JJ ASHERSON, RA KHAMASHTA, MA HUGHES, GRV TI AUTOANTIBODIES IN SYSTEMIC LUPUS-ERYTHEMATOSUS AND URTICARIAL VASCULITIS SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE ANTIENDOTHELIAL CELL ANTIBODIES; ANTI-C1Q ANTIBODIES; HYPOCOMPLEMENTEMIA; ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES ID COLLAGEN-LIKE REGION; RHEUMATIC DISEASES; ANTIBODIES; C1Q; MARKER; IGG; NEPHRITIS; CLQ AB Objective. Urticarial vasculitis (UV) is both a primary disorder and a cutaneous vasculitic manifestation in patients with connective tissue diseases. We examined the prevalence of autoantibodies to vascular endothelial cells (aECA) and anti-Clq antibodies in patients with UV. Methods. ELISA were used to detect aECA and anti-Clq autoantibodies, and we tested for correlation with UV in 4 patient groups: healthy controls, patients with systemic lupus erythematosus (SLE) with and without UV, patients with primary systemic vasculitides with UV, and patients with hypocomplementemic urticarial vasculitis syndrome (HUVS). Results. aECA were detected in 82% of patients with SLE with UV and 70% of patients with HUVS. In contrast, aECA were found in 32% of patients with SLE without UV and 14% of patients with primary UV. Anti-Clq antibodies were present in all patients with HUVS, but in < 20% in the other 3 groups. Antineutrophil cytoplasmic antibodies were detected in only one patient. Conclusion. Although the development of UV is likely to be multifactorial, the high prevalence of aECA in HUVS and SLE with UV suggests that this antibody is associated with vasculitis and may have a role in the pathogenesis of UV in these patients. C1 ST THOMAS HOSP,RAYNE INST,LUPUS ARTHRITIS RES UNIT,LONDON SE1 7EH,ENGLAND. US DEPT VET AFFAIRS,CLEVELAND,OH. CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106. NR 23 TC 18 Z9 18 U1 0 U2 1 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD SEP PY 1995 VL 22 IS 9 BP 1669 EP 1673 PG 5 WC Rheumatology SC Rheumatology GA RT927 UT WOS:A1995RT92700013 PM 8523343 ER PT J AU ROCHE, VML WELSH, CH AF ROCHE, VML WELSH, CH TI LACK OF RELATIONSHIP BETWEEN AGE, ADMISSION MEDICATIONS AND OUTCOME IN THE INTENSIVE-CARE UNIT SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract C1 UNIV COLORADO,HLTH SCI CTR,DENVER VA MED CTR,DENVER,CO. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD SEP PY 1995 VL 43 IS 9 BP SA76 EP SA76 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RT908 UT WOS:A1995RT90800332 ER PT J AU EMMONS, C AF EMMONS, C TI TRANSPORT CHARACTERISTICS OF THE APICAL ANION-EXCHANGER OF CCD BETA-INTERCALATED CELLS SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 308 EP 308 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600021 ER PT J AU NAGAMI, GT WARECH, EM AF NAGAMI, GT WARECH, EM TI EFFECT OF ACID LOADING ON ANGIOTENSIN-II-INDUCED CHANGES IN AMMONIA PRODUCTION BY THE MOUSE PROXIMAL TUBULE SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,NEPHROL SECT,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,NEPHROL SECT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 311 EP 311 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600036 ER PT J AU NAGAMI, GT WARECH, EM AF NAGAMI, GT WARECH, EM TI EFFECT OF ANGIOTENSIN-II ON AMMONIA PRODUCTION BY MOUSE S3 PROXIMAL TUBULE SEGMENTS - DIFFERENTIAL BASOLATERAL AND LUMINAL RESPONSES SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,NEPHROL SECT,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,NEPHROL SECT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 311 EP 311 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600035 ER PT J AU BROOKER, DR SMITH, PR WEISER, PC MCNULTY, KA MACKLER, SA KLEYMAN, TR AF BROOKER, DR SMITH, PR WEISER, PC MCNULTY, KA MACKLER, SA KLEYMAN, TR TI CLONING OF MOUSE EPITHELIAL NA CHANNEL AND LOCALIZATION IN THE URINARY-BLADDER SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV PENN,COLL MED,DEPT MED,PHILADELPHIA,PA 19104. UNIV PENN,COLL MED,DEPT PHYSIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. SYRACUSE UNIV,DEPT BIOL,SYRACUSE,NY 13244. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 333 EP 333 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600123 ER PT J AU KLEYMAN, TR LIN, CM MCNULTY, KA GOMEZ, LM WORRELL, RT EATON, DC LING, BN AF KLEYMAN, TR LIN, CM MCNULTY, KA GOMEZ, LM WORRELL, RT EATON, DC LING, BN TI EXPRESSION OF THE CYSTIC-FIBROSIS PHENOTYPE IN A RENAL AMPHIBIAN EPITHELIAL-CELL LINE (A6) SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV PENN,DEPT MED,DIV RENAL,PHILADELPHIA,PA 19104. UNIV PENN,DEPT PHYSIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. EMORY UNIV,ATLANTA,GA 30322. VET AFFAIRS MED CTR,ATLANTA,GA 30033. NR 0 TC 5 Z9 5 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 342 EP 342 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600159 ER PT J AU FATH, KR BURGESS, DR HAMMOND, TG AF FATH, KR BURGESS, DR HAMMOND, TG TI CYTOSKELETAL MOTOR DEPENDENCE OF RAT RENAL ENDOSOMAL FUSION - MEDIATION BY DYNEIN BUT NOT MYOSIN-I SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV PITTSBURGH,PITTSBURGH,PA. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. UNIV WISCONSIN,MADISON,WI. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 374 EP 374 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600288 ER PT J AU HAMMOND, TG VERROUST, PJ AF HAMMOND, TG VERROUST, PJ TI THE INTERNALIZATION SEQUENCE OF RENAL CORTICAL SCAVENGER PATHWAY RECEPTORS EFFECT FUSION OF MEMBRANES IN WHICH THEY RESIDE VIA DIRECT INTERACTIONS WITH H+-ATPASE SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. HOP TENON,INSERM,U64,F-75970 PARIS,FRANCE. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 375 EP 375 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600289 ER PT J AU CHERTOW, GM LAZARUS, JM CHRISTIANSEN, CL COOK, EF HAMMERMEISTER, KE GROVER, F DALEY, J AF CHERTOW, GM LAZARUS, JM CHRISTIANSEN, CL COOK, EF HAMMERMEISTER, KE GROVER, F DALEY, J TI ACUTE-RENAL-FAILURE FOLLOWING CARDIAC-SURGERY SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DIV RENAL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BROCKTON W ROXBURY VET AFFAIRS MED CTR,HSR&D,RENAL SECT,BOSTON,MA 02115. UNIV COLORADO,HLTH SCI CTR,DENVER VA MED CTR,DIV CARDIOL,DENVER,CO 80202. UNIV COLORADO,HLTH SCI CTR,DENVER VA MED CTR,DIV CARDIOTHORAC SURG,DENVER,CO 80202. NR 0 TC 2 Z9 2 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 460 EP 460 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68600632 ER PT J AU IGARASHI, G WHYTE, DA NAGAMI, GT PAYNE, JA FORBUSH, B AF IGARASHI, G WHYTE, DA NAGAMI, GT PAYNE, JA FORBUSH, B TI KIDNEY-CELL-SPECIFIC ACTIVITY OF THE MURINE NA-K-CL COTRANSPORTER (NKCC2) PROMOTER SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 YALE UNIV,SCH MED,NEW HAVEN,CT. W LOS ANGELES DVAMC,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 697 EP 697 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68601580 ER PT J AU GRANDALIANO, G ABBOUD, HE AF GRANDALIANO, G ABBOUD, HE TI THROMBIN-INDUCED DNA-SYNTHESIS IN HUMAN MESANGIAL CELLS (HMC) IS G-PROTEIN DEPENDENT AND PLATELET-DERIVED GROWTH-FACTOR (PDGF) INDEPENDENT SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV BARI,BARI,ITALY. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RI Grandaliano, Giuseppe/G-2963-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 736 EP 736 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68601735 ER PT J AU CHOUDHURY, GG MARRA, F KIYOMOTO, H ABBOUD, HE AF CHOUDHURY, GG MARRA, F KIYOMOTO, H ABBOUD, HE TI PLATELET-DERIVED GROWTH-FACTOR (PDGF) STIMULATES TYROSINE PHOSPHORYLATION OF JANUS-KINASE-1 (JAK-1) PROTEIN-TYROSINE KINASE IN HUMAN MESANGIAL CELLS (MC) SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 765 EP 765 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68601849 ER PT J AU CHOUDHURY, GG MARRA, F ABBOUD, HE AF CHOUDHURY, GG MARRA, F ABBOUD, HE TI ASSOCIATION OF THE SH2-SH3 DOMAIN-CONTAINING ADAPTER PROTEIN NCK WITH FOCAL ADHESION KINASE IN THROMBIN-STIMULATED MESANGIAL CELLS (MC) SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 786 EP 786 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68601935 ER PT J AU WENZEL, UO BHANDARI, B CHOUDHURY, GG VALENTE, AJ ABBOUD, HE AF WENZEL, UO BHANDARI, B CHOUDHURY, GG VALENTE, AJ ABBOUD, HE TI REPORTER MESANGIAL CELL-LINE TRANSFECTED WITH A MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1) PROMOTER - TRANSCRIPTIONAL ACTIVATION BY THROMBIN SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 858 EP 858 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602218 ER PT J AU BARNES, JL MITCHELL, RJ TORRES, ES AF BARNES, JL MITCHELL, RJ TORRES, ES TI EXPRESSION OF CELLULAR FIBRONECTIN (FN-EIIIA) AND THROMBOSPONDIN (TSP) DURING REMODELING IN HABU SNAKE-VENOM (HV)-INDUCED GLOMERULONEPHRITIS SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 861 EP 861 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602232 ER PT J AU KIYOMOTO, H CHOUDHURY, GG ABBOUD, HE AF KIYOMOTO, H CHOUDHURY, GG ABBOUD, HE TI STIMULATION OF MITOGEN-ACTIVATED PROTEIN-KINASE (MAPK) AND SUPPRESSION OF PROTEIN-TYROSINE-PHOSPHATASE (PTPASE) IN EXPERIMENTAL PROLIFERATIVE GLOMERULONEPHRITIS SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 872 EP 872 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602275 ER PT J AU JARA, A FELSENFELD, A FU, YS RODRIGUEZ, I KLEEMAN, CR AF JARA, A FELSENFELD, A FU, YS RODRIGUEZ, I KLEEMAN, CR TI PARATHYROID-GLAND PATHOLOGY IN AZOTEMIC RATS WITH MARKED SECONDARY HYPERPARATHYROIDISM SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT PATHOL,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 965 EP 965 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602646 ER PT J AU KASINATH, BS ABBOUD, HE MALDONADO, R TERHUNE, WC AF KASINATH, BS ABBOUD, HE MALDONADO, R TERHUNE, WC TI REGULATION OF GLOMERULAR ENDOTHELIAL-CELL (G-ENDO) SYNTHESIS OF GLOMERULAR-BASEMENT-MEMBRANE HEPARAN-SULFATE PROTEOGLYCAN (GBM HSPG) BY HIGH GLUCOSE MEDIUM SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 1042 EP 1042 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602955 ER PT J AU PARK, IS KIYOMOTO, H ABBOUD, HE AF PARK, IS KIYOMOTO, H ABBOUD, HE TI INCREASED EXPRESSION OF RENAL TGF-BETA(1) AND ALPHA-1-(IV) COLLAGEN MESSENGER-RNAS AND PROTEINS IN EARLY STREPTOZOTOCIN-INDUCED DIABETES SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV NEPHROL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 1046 EP 1046 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602970 ER PT J AU PARK, IS KIYOMOTO, H ABBOUD, SL ABBOUD, HE AF PARK, IS KIYOMOTO, H ABBOUD, SL ABBOUD, HE TI INCREASED EXPRESSION OF TRANSFORMING GROWTH-FACTOR-BETA-1 (TGF-BETA-1) AND INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS (IGFBPS) IN EARLY STREPTOZOTOCIN-INDUCED DIABETES SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD SEP PY 1995 VL 6 IS 3 SI SI BP 1046 EP 1046 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA RX686 UT WOS:A1995RX68602971 ER PT J AU HOLMAN, WL SPRUELL, RD FERGUSON, ER CLYMER, JJ VICENTE, WVA MURRAH, CP PACIFICO, AD AF HOLMAN, WL SPRUELL, RD FERGUSON, ER CLYMER, JJ VICENTE, WVA MURRAH, CP PACIFICO, AD TI TISSUE OXYGENATION WITH GRADED DISSOLVED-OXYGEN DELIVERY DURING CARDIOPULMONARY BYPASS SO JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY LA English DT Article ID EXTREME HEMODILUTION; CONSUMPTION; METABOLISM; TRANSPORT; FLOW AB Background: Intravascular perfluorochemical emulsions together with a high oxygen tension may increase the delivery of dissolved oxygen to useful levels, The hypothesis of this study is that increasing the dissolved oxygen content of blood with incremental doses of a perfluorochemical emulsion improves tissue oxygenation during cardiopulmonary bypass in a dose-related fashion, Methods and Results: Oxygen utilization was studied in a profoundly anemic canine model of hypothermic cardiopulmonary bypass, Forty-two dogs underwent normovolemic hemodilution to a hematocrit of 15.8% +/- 0.6% (mean +/- standard error of the mean), Cardiopulmonary bypass was begun and resulted in a hematocrit of 9.4% +/- 0.6%. A standard priming solution was used in the control group (n = 12), and the test groups received 1.35 gm perfluorochemical . kg(-1) (n = 10 dogs), 2.7 gm perfluorochemical . kg(-1) (n = 10 dogs), or 5.4 gm perfluorochemical . kg(-1) (n = 10 dogs) through the venous return cannula, Each animal underwent a series of randomized pump flows (0.25, 0.5, 1.0, 1.5, 2.0, and 3.0 L . min(-1). m(-2)) at 32 degrees C, After the randomized flows were completed at 32 degrees C, the temperature was raised to 38 degrees C and cardiopulmonary bypass was discontinued, Mortality from cardiac failure on separation from cardiopulmonary bypass was 42% in the control group and 20% in perfluorochemical-treated groups. The mean perfluorochemical dose was higher in survivors than in nonsurvivors (2.9 +/- 0.4 versus 1.3 +/- 0.5 gm perfluorochemical . kg(-1); p < 0.05), No differences in oxygen consumption or transbody lactate gradient were found between groups during cardiopulmonary bypass, Analysis of mixed venous oxygen tension (a surrogate measure for tissue oxygenation) as a function of cardiopulmonary bypass flow normalized to body surface area showed that the control group had significantly lower mixed venous oxygen tension (p < 0.05) than the perfluorochemical emulsion-treated groups, Furthermore, the differences were related to the perfluorochemical emulsion dose, These differences in mixed venous oxygen tension continued after termination of cardiopulmonary bypass, The coronary sinus oxygen tension and cardiac arterial-venous oxygen content differences during and after cardiopulmonary bypass were similar among the control and perfluorochemical emulsion-treated animals, Dissolved oxygen consumption during and after cardiopulmonary bypass was calculated, Dissolved oxygen consumption increased in the perfluorochemical-treated animals in a perfluorochemical dose-related manner and was significantly higher in perfluoro-chemical-treated animals than in the control animals (p < 0.05). Conclusions: Graded increases in mixed venous oxygen tension during cardiopulmonary bypass were observed in response to graded increases in the dissolved oxygen delivery. These data suggest that enhancing oxygenation with perfluorochemical-dissolved oxygen is an effective temporary substitute for the use of hemoglobin-bound oxygen during cardiopulmonary bypass. Perfluorochemical-dissolved oxygen may be particularly beneficial in the setting of multiple hypoxic stresses. C1 BIRMINGHAM VET AFFAIRS MED CTR,BIRMINGHAM,AL. ALLIANCE PHARMACEUT CORP,SAN DIEGO,CA 92121. RP HOLMAN, WL (reprint author), UNIV ALABAMA,DEPT SURG,DIV CARDIOTHORAC SURG,UNIV STN,BIRMINGHAM,AL 35294, USA. NR 31 TC 26 Z9 26 U1 0 U2 2 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0022-5223 J9 J THORAC CARDIOV SUR JI J. Thorac. Cardiovasc. Surg. PD SEP PY 1995 VL 110 IS 3 BP 774 EP 785 DI 10.1016/S0022-5223(95)70111-7 PG 12 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA RV197 UT WOS:A1995RV19700024 PM 7564446 ER PT J AU MILLER, DJ YOSHIKAWA, TT NORMAN, DC AF MILLER, DJ YOSHIKAWA, TT NORMAN, DC TI EFFECT OF AGE ON FEVER RESPONSE TO RECOMBINANT INTERLEUKIN-6 IN A MURINE MODEL SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID HYBRIDOMA GROWTH-FACTOR; BACTEREMIA; PATHOGENESIS; SURVIVAL; ADULTS; CDNA; RATS AB Background. A blunted or absent fever response to infection may occur in elderly people. Fever is mediated by endogenously produced molecules of leukocytes. The best studied of these molecules is interleukin-1 (IL-1). However, interleukin-6 (IL-6) is also known to possess the biological properties of pyrogenic cytokines. In this study, we assessed the influence of age on the febrile response to recombinant IL-6 (rIL-6) using a well-defined murine model. Methods. Balb/c male mice were injected intravenously and intraperitoneally with varying doses of rIL-6. Control mice received pyrogen-free phosphate buffered saline. Temperatures were measured rectally at baseline and at 10-minute intervals for 120 minutes post-injection using a thermistor probe. Stable baseline temperatures were first determined, and post-injection temperatures were recorded every 10 minutes for up to 120 minutes. Results. A dose-response correlation was found between the amount of the injected rIL-6 and the mean temperature changes in both young and old mice. The mean temperature changes for both young and old mice were higher following a 25 ng dose compared to a 12.5 ng dose of rIL-6 injected intravenously, and, likewise, following a 500 ng dose compared to a 250 ng dose of rIL-6 injected intraperitoneally. A significant delay in the peak temperature response was seen for both young and old mice when comparing intraperitoneal injections to intravenous injections. However, control mice showed no changes. Conclusions. Our findings confirm that IL-6 has a role in the pathogenesis of fever and that aging alters the febrile response to rIL-6. C1 W LOS ANGELES VET AFFAIRS MED CTR,CTR GERIATR RES EDUC & CLIN,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. DEPT VET AFFAIRS,OFF GERIATR & EXTENDED CARE,WASHINGTON,DC. NR 25 TC 9 Z9 9 U1 0 U2 0 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD SEP PY 1995 VL 50 IS 5 BP M276 EP M279 PG 4 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RY605 UT WOS:A1995RY60500018 PM 7671030 ER PT J AU ROBBINS, J LEVINE, R WOOD, J ROECKER, EB LUSCHEI, E AF ROBBINS, J LEVINE, R WOOD, J ROECKER, EB LUSCHEI, E TI AGE EFFECTS ON LINGUAL PRESSURE GENERATION AS A RISK FACTOR FOR DYSPHAGIA SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID SWALLOWING DISORDERS; ORAL-PHASE; PERFORMANCE AB Background. Tongue activity plays a crucial role in both oral and pharyngeal phases of swallowing. In this study, maximum lingual isometric and swallowing pressures were quantified in two groups of healthy men to investigate possible age effects on performance. Magnetic resonance images of the brain were also obtained to examine the relationship between age-related anatomical changes and swallowing function. Methods. Pressures were recorded at three lingual sites (tip, blade, and dorsum) during a maximal isometric task and during saliva swallows. Task order was randomized, and subjects performed three trials per placement site. Additionally, t2-weighted MRIs were obtained on 9 of the 10 young subjects (mean age = 25 years) and all 15 older subjects (mean age = 75 years). Results. Maximal isometric pressures were significantly greater for younger subjects at the tongue blade site (p = .002), whereas peak swallowing pressures remained similar across both age groups. Within-subject comparisons of maximum isometric to swallowing pressures, a measure of reserve capacity, revealed reduced difference scores at the tongue blade in the older group (p = .02). Older subjects exhibited significantly more cerebral atrophy (p = .001) and greater incidence of periventricular white matter lesions (p = .0001) than did younger subjects. Conclusions. While swallowing pressures remain similar across the life span, overall pressure reserve declines with age. The implications are: (a) older people may be working harder to produce adequate swallowing pressures, and (b) age-related illness may put geriatric patients at higher risk for dysphagia, thus further complicating recovery. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT NEUROL,MADISON,WI 53706. UNIV WISCONSIN,DEPT BIOSTAT,MADISON,WI 53706. UNIV IOWA,DEPT SPEECH PATHOL & AUDIOL,IOWA CITY,IA 52242. RP ROBBINS, J (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN 11G,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU FDA HHS [R01BS24427] NR 45 TC 125 Z9 127 U1 0 U2 10 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD SEP PY 1995 VL 50 IS 5 BP M257 EP M262 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RY605 UT WOS:A1995RY60500015 PM 7671027 ER PT J AU KREISBERG, JI KREISBERG, SH AF KREISBERG, JI KREISBERG, SH TI HIGH GLUCOSE ACTIVATES PROTEIN-KINASE-C AND STIMULATES FIBRONECTIN GENE-EXPRESSION BY ENHANCING A CAMP RESPONSE ELEMENT SO KIDNEY INTERNATIONAL LA English DT Article; Proceedings Paper CT 2nd Hyonam-Kidney-Laboratory International Symposium on Pathogenesis of Diabetic Nephropathy - Experimental Approaches CY JAN 21, 1995 CL SEOUL, SOUTH KOREA SP Hyonam Kidney Lab ID SIGNAL TRANSDUCTION PATHWAYS; MESANGIAL CELLS; TRANSCRIPTIONAL ACTIVATION; INDUCED BINDING; MESSENGER-RNA; CYCLIC-AMP; FAMILY; PHOSPHORYLATION; INCREASE; JUN/AP-1 C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP KREISBERG, JI (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIDDK NIH HHS [DK 29787] NR 39 TC 30 Z9 32 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD SEP PY 1995 VL 48 SU 51 BP S3 EP S11 PG 9 WC Urology & Nephrology SC Urology & Nephrology GA RR159 UT WOS:A1995RR15900002 PM 7474685 ER PT J AU BARSTOW, TJ SCREMIN, AME MUTTON, DL KUNKEL, CF CAGLE, TG WHIPP, BJ AF BARSTOW, TJ SCREMIN, AME MUTTON, DL KUNKEL, CF CAGLE, TG WHIPP, BJ TI GAS-EXCHANGE KINETICS DURING FUNCTIONAL ELECTRICAL-STIMULATION IN SUBJECTS WITH SPINAL-CORD INJURY SO MEDICINE AND SCIENCE IN SPORTS AND EXERCISE LA English DT Article DE TIME CONSTANT; EXPONENTIAL; O-2 DEFICIT; EXCESS POSTEXERCISE OXYGEN CONSUMPTION; O-2 DEBT; SPINAL CORD INJURY ID OXYGEN-UPTAKE KINETICS; SUBMAXIMAL EXERCISE; QUADRICEPS MUSCLE; HEAVY EXERCISE; ONSET; DYNAMICS; CONSTANT; FIBERS; HUMANS; REST AB We examined the kinetics of VO2, VCO2, and V-E following the onset of unloaded leg cycling, and in recovery, in sis patients with spinal cord injury (SCI). Exercise was produced by functional electrical stimulation (FES) of the quadriceps, hamstrings, and gluteal muscles. End-exercise VO2 (1.03 +/- 0.16 l . min(-1)), VCO2 (1.20 +/- 0.22 l . min(-1)) and V-E (41 +/- 10 l . min(-1)) were elevated compared to values typically seen in healthy ambulatory subjects performing similar unloaded cycling Mean response times for the on transients (MRT(on)) were both long and Variable across subjects for VO2 (165 +/- 62 s), VCO2 (173 +/- 58 s), and V-E (202 +/- 61 s). Recovery kinetics showed much less intersubject variability, and for five of sis subjects were faster than the equivalent exercise MRT for all three variables (MRT(off) for VO2 of 103 +/- 28 s, VCO2 136 +/- 20 s, and V-E 144 +/- 34 s), but P > 0.05 for all three. Size of the O-2 deficit (1.96 +/- 0.90 l) and end-exercise lactate (7.05 +/- 1.65 mmol . l(-1)) were similar to Values reported for healthy sedentary subjects performing maximal voluntary exercise, but the end-exercise heart rate (102 +/- 16 bpm) was lower than expected for this intensity of exercise. Tn conclusion, FES-induced unloaded cycling leads to exaggerated responses of pulmonary gas exchange and long time constants in patients with SCI. The delayed kinetics may be due in part to a blunted increase in heart rate in addition to severe deconditioning. C1 W LOS ANGELES VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV,ALBUQUERQUE,NM 87108. UNIV NEW MEXICO,DEPT ORTHOPED,ALBUQUERQUE,NM 87131. ST GEORGE HOSP,SCH MED,DEPT PHYSIOL,LONDON SW17 0RE,ENGLAND. RP BARSTOW, TJ (reprint author), UNIV CALIF LOS ANGELES,HARBOR MED CTR,ST JOHNS CARDIOVASC RES CTR,DEPT MED,TORRANCE,CA 90509, USA. NR 42 TC 25 Z9 25 U1 1 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0195-9131 J9 MED SCI SPORT EXER JI Med. Sci. Sports Exerc. PD SEP PY 1995 VL 27 IS 9 BP 1284 EP 1291 PG 8 WC Sport Sciences SC Sport Sciences GA RU235 UT WOS:A1995RU23500008 PM 8531627 ER PT J AU GIORDANO, M DEFRONZO, RA AF GIORDANO, M DEFRONZO, RA TI ACUTE EFFECT OF HUMAN RECOMBINANT INSULIN-LIKE GROWTH-FACTOR-I ON RENAL-FUNCTION IN HUMANS SO NEPHRON LA English DT Article DE GLOMERULAR FILTRATION RATE; PHOSPHATE; POTASSIUM; RENAL PLASMA FLOW; SODIUM ID GLOMERULAR-FILTRATION RATE; COLLECTING DUCT; MESANGIAL CELLS; GENE-EXPRESSION; PLASMA-FLOW; HORMONE; SOMATOMEDIN; MEMBRANE; GLUCOSE; KIDNEY AB We examined the acute effects of insulin-like growth factor I (IGF-I) on renal function in 8 normal subjects who received a 3-hour intravenous infusion of IGF-I at a rate of 0.4 mu g/kg . min. After starting the IGF-I infusion, the plasma IGF-I concentration rose quickly and achieved a plateau after 90 min that was threefold above the basal value. During IGF-I infusion, progressive rises in glomerular filtration rate (from 93 +/- 4 to 121 +/- 6 ml/173 m(2) . min; p < 0.01) and renal plasma flow (from 529 +/- 31 to 703 +/- 55 ml/1.73 m(2) . min; p < 0.01) were observed, with no change in filtration fraction. The plasma levels of potassium and phosphate decreased significantly during the last 90 min of the study, while the plasma sodium concentration remained unchanged. A significant decrease in the fractional excretion of sodium, potassium, and phosphate was observed during the last 90 min of the IGF-I infusion period. The heart rate rose modestly with no change in mean arterial pressure. These findings show that, when administered acutely, IGF-I is a potent renal. vasodilator, but is antinatriuretic. IGF-I also enhances potassium and phosphate uptake by extrarenal tissues and reduces renal potassium and phosphate excretion. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV DIABET,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV NAPLES 2,INST INTERNAL MED & NEPHROL,NAPLES,ITALY. NR 30 TC 33 Z9 35 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0028-2766 J9 NEPHRON JI Nephron PD SEP PY 1995 VL 71 IS 1 BP 10 EP 15 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA RT600 UT WOS:A1995RT60000002 PM 8538827 ER PT J AU VANN, JM GOLDMAN, AJ SZUREK, PF BROOKS, BR AF VANN, JM GOLDMAN, AJ SZUREK, PF BROOKS, BR TI DEOXYGLUCOSE UPTAKE BY MOUSE ASTROCYTES - EFFECTS OF TEMPERATURE AND RETROVIRUS INFECTION SO NEUROCHEMICAL RESEARCH LA English DT Article DE FIBROBLAST; MURINE LEUKEMIA VIRUS; SARCOMA VIRUS; HIV-1; POSITRON EMISSION TOMOGRAPHY ID HUMAN-IMMUNODEFICIENCY-VIRUS; ROUS-SARCOMA VIRUS; CENTRAL-NERVOUS-SYSTEM; AIDS DEMENTIA COMPLEX; CHICK-EMBRYO FIBROBLASTS; MURINE LEUKEMIA-VIRUS; HUMAN GLIAL-CELLS; SUGAR-TRANSPORT; HEXOSE-TRANSPORT; GLUCOSE-UPTAKE AB Deoxyglucose uptake by FVB/N mouse astrocytes was studied before and after infection by ts1 retrovirus which causes a neurodegenerative disease in mice similar to HIV-1 encephalopathy in man. The Michaelis-Menten kinetic parameters, Km and Vmax, of 2-deoxy-D-glucose uptake by brain and cerebellar astrocytes were measured following culture al 34 degrees C where ts1 retrovirus replicates optimally, and at 37 degrees C. Compared to astrocytes cultured at 37 degrees C, astrocytes cultured at 34 degrees C had increased Km and decreased deoxyglucose uptake despite increased or unchanged Vmax. Following ts1 retrovirus infection, brain astrocyte deoxyglucose uptake doubled [132%] associated with decreased Km but unchanged Vmax, whereas cerebellar astrocyte deoxyglucose uptake doubled [102%] associated with increased Vmax but unchanged Km. These observations of altered deoxyglucose uptake kinetic parameters following retrovirus infection indicate different neurochemical mechanisms for the regional variation in deoxyglucose uptake observed following retrovirus infection of the CNS in vivo. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,NEUROL SERV,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,RES SERV,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT NEUROL MED MICROBIOL,MADISON,WI. UNIV WISCONSIN,SCH MED,DEPT IMMUNOL,MADISON,WI. NR 40 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PUBL CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0364-3190 J9 NEUROCHEM RES JI Neurochem. Res. PD SEP PY 1995 VL 20 IS 9 BP 1013 EP 1020 DI 10.1007/BF00995554 PG 8 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA RX664 UT WOS:A1995RX66400006 PM 8570004 ER PT J AU MATTAMMAL, MB HARING, JH CHUNG, HD RAGHU, G STRONG, R AF MATTAMMAL, MB HARING, JH CHUNG, HD RAGHU, G STRONG, R TI AN ENDOGENOUS DOPAMINERGIC NEUROTOXIN - IMPLICATION FOR PARKINSONS-DISEASE SO NEURODEGENERATION LA English DT Article DE CELL DEATH; DOPAMINE; METABOLISM; NEUROTOXIN; PARKINSONISM ID NERVE GROWTH-FACTOR; PHEOCHROMOCYTOMA CELLS; BRAIN; GLUTATHIONE; NEURONS; 1-METHYL-4-PHENYLPYRIDINIUM; N-METHYL-4-PHENYLPYRIDINE; HYPOTHESIS; METABOLITE; ETIOLOGY AB Oxidation of dopamine by monoamine oxidase results in the endogenous metabolite 3,4-dihydroxyphenylacetaldehyde (DOPAL). The toxicity of DOPAL for dopaminergic neurons was investigated using rat neostriatal synaptosomes, PC-12 cells and cultures of fetal rat dissociated mesencephalon. The Na+-dependent uptake of [H-3]DOPAL in synaptosomes was inhibited by mazindol. DOPAL selectively inhibited dopamine uptake but not [C-14]GABA uptake, induced membrane damage and liberation of dopamine into the medium. Incubation of PC-12 cells with 6.5 mu M of DOPAL for 24 h caused degeneration of the neuritic process, and the number of viable cells were reduced by 25% of control. There were practically no surviving cells after 24 h of incubation with 33 mu M of DOPAL. After 8 h of treatment with 33 mu M of DOPAL, dopamine and 3,4-dihydroxyphenylacetic acid content in the cells were reduced by 38% and 53% of control. DOPAL-induced cell damage released lactic acid dehydrogenase into the incubation media. This toxic effect of DOPAL was time- and concentration-dependent. In mesencephalic cultures, after exposure to 33 mu M of DOPAL, the surviving TH+ cells showed rounded cell body, and fibre network was highly reduced. These results indicate DOPAL is a neurotoxin and may be involved in the degeneration of dopaminergic neurons. (C) 1995 Academic Press Limited C1 VET ADM MED CTR, CLIN LAB SERV, ST LOUIS, MO 63125 USA. ST LOUIS UNIV, HLTH SCI CTR, DEPT INTERNAL MED, ST LOUIS, MO 63104 USA. ST LOUIS UNIV, HLTH SCI CTR, DEPT PATHOL, ST LOUIS, MO 63104 USA. ST LOUIS UNIV, HLTH SCI CTR, DEPT ANAT & NEUROBIOL, ST LOUIS, MO 63104 USA. AUDIE L MURPHY MEM VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT CELL & STRUCT BIOL, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT PHARMACOL, SAN ANTONIO, TX 78284 USA. RP VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN 11G JB, ST LOUIS, MO 63125 USA. NR 37 TC 65 Z9 65 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1055-8330 J9 NEURODEGENERATION JI Neurodegeneration PD SEP PY 1995 VL 4 IS 3 BP 271 EP 281 DI 10.1016/1055-8330(95)90016-0 PG 11 WC Neurosciences SC Neurosciences & Neurology GA RX774 UT WOS:A1995RX77400004 PM 8581559 ER PT J AU NETSCHER, DT WIGODA, P THORNBY, J YIP, B RAPPAPORT, NH AF NETSCHER, DT WIGODA, P THORNBY, J YIP, B RAPPAPORT, NH TI THE HEMODYNAMIC AND HEMATOLOGIC EFFECTS OF CIGARETTE-SMOKING VERSUS A NICOTINE PATCH SO PLASTIC AND RECONSTRUCTIVE SURGERY LA English DT Article ID BLOOD-FLOW; BREAST RECONSTRUCTION; TOBACCO SMOKING; FACE LIFT; SKIN; COMPLICATIONS; FIBRINOLYSIS; FIBRINOGEN; PATIENT; SMOKERS AB Patients who smoke have higher complication rates than nonsmokers in the postoperative period. The authors designed an experimental protocol for habitual smokers (n = 30) to determine the specific hemodynamic and hematologic adverse effects possibly caused by nicotine and whether the method of nicotine delivery and systemic nicotine levels achieved might influence these adverse effects. During the 5-day study, subjects were asked to refrain from smoking, and testing sessions were conducted as follows: on day 1, the subjects smoked two cigarettes immediately before testing; on day 3 (control day), testing was done after not smoking for 48 hours and then the subjects were instructed to wear a transdermal nicotine patch (PROSTEP 22 mg/day) for 24 hours and replace it with another so that, on day 5, testing took place after the subjects had worn the patch for approximately 34 hours. At each testing session, digital artery pulse amplitude and a number of clinical and serum blood level parameters were measured. Relative digital blood flow after smoking (69.2 +/- 5.8%) and with the patch (80.4 +/- 7.6%) was lower than on the control day (100.0 +/- 0.0%). The nicotine patch, unlike smoking, had no effect on vasopressin or fibrinogen concentrations, hematocrit, or white cell or platelet counts; both smoking and the patch resulted in elevated norepinephrine levels. C1 BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. DEPT VET AFFAIRS MED CTR,HOUSTON,TX. UNIV TEXAS,SW MED CTR,DALLAS,TX. NR 37 TC 28 Z9 28 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0032-1052 J9 PLAST RECONSTR SURG JI Plast. Reconstr. Surg. PD SEP PY 1995 VL 96 IS 3 BP 681 EP 688 DI 10.1097/00006534-199509000-00022 PG 8 WC Surgery SC Surgery GA RP607 UT WOS:A1995RP60700022 PM 7638293 ER PT J AU KIM, J MODLIN, RL MOY, RL DUBINETT, SM MCHUGH, T NICKOLOFF, BJ UYEMURA, K AF KIM, J MODLIN, RL MOY, RL DUBINETT, SM MCHUGH, T NICKOLOFF, BJ UYEMURA, K TI IL-10 PRODUCTION IN CUTANEOUS BASAL AND SQUAMOUS-CELL CARCINOMAS - A MECHANISM FOR EVADING THE LOCAL T-CELL IMMUNE-RESPONSE SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TUMOR INFILTRATING LYMPHOCYTES; INHIBITS CYTOKINE PRODUCTION; EXPRESSION; INTERFERON; CANCER; PROFILES; CLONES; TH1; INTERLEUKIN-10; IMMUNOTHERAPY AB Cytokines play a vital role in the host immune response by regulating the development and function of immunocompetent cells. To explore the possibility that tumors may alter the host response via release of immuno-modulatory cytokines, we studied two different skin cancers, basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) as models. By RT-PCR, we found that the type 2 cytokines, IL-4 and IL-10, were strongly expressed in BCC compared with matched PBMC. Furthermore, IL-10 was more strongly expressed in SCC compared with benign growths. To identify the cell types responsible for production of these cytokines, tumor and tumorinfiltrating lymphocyte (TIL) cell lines were derived from BCC and SCC biopsy specimens. IL-2 and IFN-gamma mRNAs were expressed in TIL, while IL-10 mRNA were strongly expressed in BCC lines. In addition, IL-10 was detected in culture supernatants from BCC and SCC cell lines by ELISA and in tissue sections by immunohistology. TIL lines derived from these tumors demonstrated proliferative activity to autologous tumor cells in the presence of APCs, dependent on the addition of low concentrations of rIL-2 or neutralizing anti-IL-10 mAb in the culture medium. Furthermore, treatment of BCC with intralesional IFN-alpha induced tumor regression with concomitant up-regulation of IL-2 and down-regulation of IL-10 mRNA expression in lesions. These data suggest that tumor production of the cytokine, IL-10, may provide a mechanism for evading the local T cell-mediated immune response. C1 UNIV CALIF LOS ANGELES,SCH MED,DIV DERMATOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,JONSSON COMPREHENS CANC CTR,LOS ANGELES,CA 90024. UNIV MICHIGAN,DEPT PATHOL,ANN ARBOR,MI 48109. OI Modlin, Robert/0000-0003-4720-031X FU NCI NIH HHS [CA56860]; NIAID NIH HHS [AI22553]; NIAMS NIH HHS [AR31957] NR 29 TC 240 Z9 253 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD AUG 15 PY 1995 VL 155 IS 4 BP 2240 EP 2247 PG 8 WC Immunology SC Immunology GA RN464 UT WOS:A1995RN46400062 PM 7636270 ER PT J AU STERNINI, C SU, D GAMP, PD BUNNETT, NW AF STERNINI, C SU, D GAMP, PD BUNNETT, NW TI CELLULAR SITES OF EXPRESSION OF THE NEUROKININ-1 RECEPTOR IN THE RAT GASTROINTESTINAL-TRACT SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE EXCITATORY MOTOR-NEURONS; INTERNEURONS; SENSORY NEURONS; TACHYKININS; NEUROKININ RECEPTORS ID GUINEA-PIG ILEUM; ENTERIC NERVOUS-SYSTEM; VASOACTIVE INTESTINAL POLYPEPTIDE; SUBSTANCE-P; CIRCULAR MUSCLE; BINDING-SITES; PEPTIDE IMMUNOREACTIVITY; AUTORADIOGRAPHIC LOCALIZATION; MOLECULAR CHARACTERIZATION; TACHYKININ RECEPTORS AB In the digestive system, substance P is an excitatory transmitter to muscle, a putative excitatory neuro-neuronal transmitter, a vasodilator, and a mediator in inflammatory processes. Many of the biological effects of substance P are mediated by a high-affinity interaction with the tachykinin receptor neurokinin-1. The aim of the present study was to identify the sites of expression of this receptor in the rat stomach and intestine by immunohistochemistry with a polyclonal antiserum raised to the intracellular C-terminal portion of the rat neurokinin-1 receptor. Neurokinin-1 receptor immunoreactivity is present in a large population of enteric neurons. The relative density of these neurons along the gut is colon > ileum >> stomach. In the intestine, stained neurons have a smooth cell body with processes that can be followed within and between plexuses, and make close approaches to other neuronal cells, but do not appear to project outside the plexuses, suggesting that they are interneurons. In the stomach, neurokinin-1 receptor-immunoreactive neurons are infrequent and have a poorly defined and irregular shape. Neurokinin-1 receptor immunoreactivity is also localized to numerous non-neuronal cells in the inner portion of the circular muscle layer of the small intestine, which have the appearance of small dark smooth muscle cells or interstitial cells of Cajal. These cells are postulated to form a ''stretch-sensitive'' system with the deep muscular plexus and thus constitute an important site of regulation of muscle activity. Double labeling immunofluorescence was used to simultaneously localize neurokinin-1 receptor and substance P/tachykinin immunoreactivities. These experiments demonstrate that in the enteric plexuses, substance P/tachykinin-immunoreactive varicose fibers encircle the cell bodies of most neurokinin-1 receptor-containing neurons, and in the inner portion of the circular muscle layer of the small intestine they lie close to neurokinin-1 receptor-immunoreactive non-neuronal cells. In addition, some enteric neurons express both neurokinin-1 receptor and substance P/tachykinin immunoreactivities. The present study provides strong evidence that the neurokinin-1 receptor is the tachykinin receptor mediating the actions of substance P on enteric neurons and smooth muscle. (C) 1995 Wiley-Liss, Inc. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024. UNIV CALIF SAN FRANCISCO,DEPT SURG,SAN FRANCISCO,CA 94143. UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,SAN FRANCISCO,CA 94143. RP STERNINI, C (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CURE VA UCLA GASTROENTEROL BIOL CTR,BLDG 115,ROOM 224,LOS ANGELES,CA 90073, USA. FU NIDDK NIH HHS [DK 41301, DK 38752, DK 43207] NR 57 TC 134 Z9 135 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD AUG 7 PY 1995 VL 358 IS 4 BP 531 EP 540 DI 10.1002/cne.903580406 PG 10 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA RL458 UT WOS:A1995RL45800005 PM 7593747 ER PT J AU DHANDA, R MULROW, CD GERETY, MB LEE, S CORNELL, JE AF DHANDA, R MULROW, CD GERETY, MB LEE, S CORNELL, JE TI CLASSIFYING CHANGE WITH THE SICKNESS IMPACT PROFILE - FOR NURSING-HOMES (SIP-NH) SO AGING-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE FRAIL ELDERLY; FUNCTIONAL STATUS; HEALTH STATUS MEASURES; NURSING HOMES; QUESTIONNAIRES AB The aim of this cohort study was to evaluate the concordance of the Sickness Impact Profile for Nursing Homes (SIP-NH) and Sickness Impact Profile (SIP) in classifying change. Subjects consisted of 194 consecutive long-stay nursing home residents at one academic department of the V.A. and in 8 community proprietary nursing homes in San Antonio, Texas. They were to have more than 3 months residency; to be greater than or equal to 61 years; and to be dependent in at least 2 ADLs with an MMSE score of greater than or equal to 15. Subjects were administered a 128-item SIP and a reduced 66-item SIP-NH at baseline and 4, 8, and 12-month follow-up. At each follow-up, subjects were classified into 3 mutually exclusive change categories using a change score of greater than or equal to 5 points. Concordance of the classification of subjects by the SIP-NH an SIP was evaluated. The misclassification rate as well as its direction was also assessed. Both instruments classified a little over one-quarter of the subjects as better, over a third as being unchanged, and another third as being worse at the four-month follow-up. More subjects were classified as worse by both in struments at 8 and 12 months. All kappas ranged from 0.52 to 0.78, indicating good to excellent agreement. Overall, the SIP-NH characterized persons as changed more often than the SIP with no systematic directional bias. In conclusion, the SIP-NH was concordant with the SIP in classifying change in subjects. However, Lye cannot say which of the two is better for detecting change. Future research must focus on defining a change score which has clinical meaning, and evaluate responsiveness to change. C1 UNIV TEXAS,HLTH SCI CTR,DIV GEN MED,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DIV GERIATR & GERONTOL,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,CTR AGING RES & EDUC,SAN ANTONIO,TX. RP DHANDA, R (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. FU NIA NIH HHS [U01AG09117] NR 0 TC 2 Z9 2 U1 0 U2 0 PU EDITRICE KURTIS S R L PI MILANO PA VIA LUIGI ZOJA, 30-20153 MILANO, ITALY SN 0394-9532 J9 AGING-CLIN EXP RES JI Aging-Clin. Exp. Res. PD AUG PY 1995 VL 7 IS 4 BP 228 EP 233 PG 6 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA RY072 UT WOS:A1995RY07200011 PM 8541376 ER PT J AU MORRISON, D CROWLEY, ST BIES, R BARBIERE, CC AF MORRISON, D CROWLEY, ST BIES, R BARBIERE, CC TI SYSTOLIC BLOOD-PRESSURE RESPONSE TO PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTY FOR CARDIOGENIC-SHOCK SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID ACUTE MYOCARDIAL-INFARCTION AB This study reports that an improvement in systolic blood pressure of >10 mm Hg after percutaneous transluminal coronary angioplasty in patients with cardiogenic shock was associated with in-hospital survival (8 of 8 patients), Failure to achieve infarct artery patency (6 of 6 patients) or technically successful percutaneous transluminal coronary angioplasty unaccompanied by improved systolic blood pressure in the laboratory were both accompanied by in-hospital mortality in this series. C1 UNIV COLORADO,HLTH SCI CTR,DENVER,CO 80220. RP MORRISON, D (reprint author), DENVER VET AFFAIRS MED CTR,CARDIOL SECT,1055 CLERMONT ST,DENVER,CO 80220, USA. NR 6 TC 5 Z9 5 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD AUG 1 PY 1995 VL 76 IS 4 BP 313 EP 314 DI 10.1016/S0002-9149(99)80090-3 PG 2 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA RL359 UT WOS:A1995RL35900023 PM 7618633 ER PT J AU LIU, AW DELGADOESCUETA, AV SERRATOSA, JM ALONSO, ME MEDINA, MT GEE, MN CORDOVA, S ZHAO, HZ SPELLMAN, JM PEEK, JRR DONNADIEU, FR SPARKES, RS AF LIU, AW DELGADOESCUETA, AV SERRATOSA, JM ALONSO, ME MEDINA, MT GEE, MN CORDOVA, S ZHAO, HZ SPELLMAN, JM PEEK, JRR DONNADIEU, FR SPARKES, RS TI JUVENILE MYOCLONIC EPILEPSY LOCUS IN CHROMOSOME 6P21.2-P11 - LINKAGE TO CONVULSIONS AND ELECTROENCEPHALOGRAPHY TRAIT SO AMERICAN JOURNAL OF HUMAN GENETICS LA English DT Article ID IDIOPATHIC GENERALIZED EPILEPSY; NORMAL-CHILDREN; HLA REGION; FAMILIES AB Despite affecting 4 million Americans and 100-200 million persons worldwide, the precise molecular mechanisms of human epilepsies remain unknown. Juvenile myoclonic epilepsy (JME) is the most frequent and, hence, most important form of hereditary grand mal epilepsy. In this epilepsy, electroencephalographic (EEG) 15-30-Hz multispikes produce myoclonic and tonic-clonic convulsions beginning at 8-20 years of age. Moreover, EEG 3.5-6-Hz multispike wave complexes appear in clinically asymptomatic family members. We first studied 38 members of a four-generation LA-Belize family with classical JME but with no pyknoleptic absences. Five living members had JME; four clinically asymptomatic members had EEG multispike wave complexes. Pairwise analysis tightly linked microsatellites centromeric to HLA, namely D6S272 (peak lod score [Z(max)] = 3.564-3.560 at male-female recombination [theta(m=f)] = 0.001) and D6S257 (Z(max) = 3.672-3.6667 at theta(m=f) = 0.001), spanning 7 cM, to convulsive seizures and EEG multispike wave complexes. A recombination between D6S276 and D6S273 in one affected member placed the JME locus within or below HLA. Pairwise, multipoint, and recombination analyses in this large family independently proved that a JME gene is located in chromosome 6p, centromeric to HLA. We next screened, with the same chromosome 6p21.2-p11 short tandem-repeat polymorphic markers, seven multiplex pedigrees with classic JME, When led scores for small multiplex families are added to led scores of the LA-Belize pedigree, Z(max) values for D6S294 and D6S257 are >7 (theta(m=f) = .000). Our results prove that in chromosome 6p21.2-p11 an epilepsy locus exists whose phenotype consists of classic JME with convulsions and/or EEG rapid multispike wave complexes. C1 W LOS ANGELES VET AFFAIRS MED CTR,VET AFFAIRS SW REG EPILEPSY CTR,NEUROL SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,VET AFFAIRS SW REG EPILEPSY CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,CALIF COMPREHENS EPILEPSY PROGRAM,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA. NATL AUTONOMOUS UNIV,TEGUCIGALPA,HONDURAS. NATL INST NEUROL & NEUROSURG,MEXICO CITY,DF,MEXICO. FU NINDS NIH HHS [5P01 NS21908] NR 31 TC 115 Z9 116 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0002-9297 J9 AM J HUM GENET JI Am. J. Hum. Genet. PD AUG PY 1995 VL 57 IS 2 BP 368 EP 381 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA RL771 UT WOS:A1995RL77100020 PM 7668263 ER PT J AU UY, HL REASNER, CA SAMUELS, MH AF UY, HL REASNER, CA SAMUELS, MH TI PATTERN OF RECOVERY OF THE HYPOTHALAMIC-PITUITARY-THYROID AXIS FOLLOWING RADIOACTIVE IODINE THERAPY IN PATIENTS WITH GRAVES-DISEASE SO AMERICAN JOURNAL OF MEDICINE LA English DT Article ID THYROTROPIN-RELEASING-HORMONE; LONG-TERM FOLLOW; FEEDBACK-REGULATION; I-131 THERAPY; HYPERTHYROIDISM; SECRETION; TSH; THYROTOXICOSIS; HYPOTHYROIDISM; SUPPRESSION AB PURPOSE: To characterize the time course of recovery of the hypothalamic-pituitary-thyroid (HPT) axis by determining the frequency, onset, duration, and clinical attributes of the central hypothyroid phase following I-131 therapy for Graves' disease and to examine whether the central hypothyroid phase is due to direct pituitary thyrotroph suppression or to hypothalamic thyrotropin-releasing hormone (TRH) deficiency. PATIENTS AND METHODS: Twenty-one hyperthyroid patients with Graves' disease evaluated at a university endocrine clinic and treated with radioactive iodine were prospectively studied. Serial thyroid function levels (sevum thyroxine [T-4], free thyroxine [free T-4], triiodothyronine [T-3], and thyroid-stimulating hormone [TSH]) were measured and TRH stimulation tests were performed at 2 to 4 week intervals for all subjects following I-131 treatment. None of the patients was treated with thionamides after receiving I-131 therapy. RESULTS: Nineteen (90%) of the patients with Graves' disease experienced a transient central hypothyroid phase defined as the presence of a suppressed or inappropriately normal TSH level despite a low free T-4 level following I-131 treatment. This phase occurred a mean of 62.8 +/- 5.1 days following I-131 treatment, persisted for an average of 24.7 +/- 2.4 days, and was not predictive of eventual treatment outcome. All patients had concordantly low T-4 and T-3 levels during this period and exhibited a blunted TSH response to TRH compared to 29 euthyroid control subjects, suggesting primary feedback suppression at the level of the pituitary thyrotrophs. The suppressed thyrotrophs required a minimum of 2 weeks to recover once patients became hypothyroid. The length of preexisting hyperthyroidism, basal free T-4 elevation, and administered dose of I-131 failed to predict the duration of the central hypothyroid phase, although a higher dose of I-131 was associated with an earlier onset of central hypothyroidism (r = -.51, P <0.05). CONCLUSIONS: Clinicians should be aware of the delay in the recovery of the HPT axis that occurs in the majority of patients with Graves' disease treated with I-131 and is manifested by a transient central hypothyroid phase. The blunted TSH response to TRH stimulation during this period suggests that suppression occurs primarily at the level of the pituitary thyrotrophs. The use of sensitive TSH measurements alone to monitor these patients during this period is not helpful and may be misleading. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP UY, HL (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL & METAB,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NCRR NIH HHS [MO1-RR-01345] NR 30 TC 39 Z9 42 U1 0 U2 1 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD AUG PY 1995 VL 99 IS 2 BP 173 EP 179 DI 10.1016/S0002-9343(99)80137-5 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA RN319 UT WOS:A1995RN31900010 PM 7625422 ER PT J AU WANG, DH BALKOVETZ, DF WARNOCK, DG AF WANG, DH BALKOVETZ, DF WARNOCK, DG TI MUTATIONAL ANALYSIS OF TRANSMEMBRANE HISTIDINES IN THE AMILORIDE-SENSITIVE NA+/H+ EXCHANGER SO AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY LA English DT Article DE SODIUM HYDROGEN EXCHANGER; CIMETIDINE; DIETHYL PYROCARBONATE; ETHYL ISOPROPYL AMILORIDE ID RAT NA/H EXCHANGER; BRUSH-BORDER MEMBRANE; H+ EXCHANGER; MOLECULAR-CLONING; FUNCTIONAL EXPRESSION; CHEMICAL MODIFICATION; ESCHERICHIA-COLI; CYTOSOLIC PH; ANTIPORTER; NHE-1 AB The histidine-reactive reagent, diethyl pyrocarbonate (DEPC) inhibits the human amiloride-sensitive Na+/H+ exchanger (NHE1) in stably transfected fibroblasts. NHE1 was protected by cimetidine and amiloride from DEPC, and DEPC inhibition was reversed with hydroxylamine, suggesting a role for critical histidine groups in NHE activity. We replaced the histidines CH) in putative transmembrane domains (H35, H120, H349) with glycine (G) using site-directed mutagenesis. There was no significant change in NHE activity of the H120G; H349G; H120,349G; and H35,120,349G mutants compared with wild type. The 50% inhibition concentration values for amiloride, ethyl isopropyl amiloride (EIPA), and cimetidine of the H349G mutant were significantly increased compared with the wildtype NHE1. We also examined the DEPC effect on the transport activity of the triple histidine mutant (H35,120,349G) and found that NHE1 activity was still inhibited by DEPC with reversal by hydroxylamine and protected by amiloride and cimetidine. Kinetic analysis of DEPC inhibition indicated that two ''critical'' histidine residues are required for NHE transport activity. Substitutions of H349 with asparagine (N), glutamine (Q), serine (S), tyrosine (Y), valine (V), leucine (L), and phenylalanine (F) were also examined. There were no changes in NHE activity of these mutants compared with wild type. The H349G and H349L mutants became more resistant to amiloride, whereas the H349Y and H349F mutants became more sensitive to amiloride. The H349S (mimics NHE3) and H349Y (mimics NHE4) mutations had only modest effects on amiloride sensitivity. These results indicate that H349 affects the interaction of NHE1 with its inhibitors, even though substitutions at this site, per se, do not appear to explain the differences in amiloride sensitivity between different NHE isoforms. Despite clear-cut effects of the H349G mutation on the competitive interaction of NHE1 with cimetidine and EIPA, this mutation did not affect the affinity of NHE1 for its cationic substrates (Na+, Li+). C1 UNIV ALABAMA, DEPT MED, DIV NEPHROL, BIRMINGHAM, AL 35294 USA. UNIV ALABAMA, VASC BIOL & HYPERTENS PROGRAM, CTR NEPHROL RES & TRAINING, DEPT PHYSIOL & BIOPHYS, BIRMINGHAM, AL 35294 USA. UNIV ALABAMA, BIRMINGHAM VET AFFAIRS MED CTR, DEPT VET AFFAIRS, BIRMINGHAM, AL 35294 USA. FU NHLBI NIH HHS [5T32-HL-07457]; NIDDK NIH HHS [R01-DK-19407, P50-DK-39258] NR 47 TC 53 Z9 53 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6143 J9 AM J PHYSIOL-CELL PH JI Am. J. Physiol.-Cell Physiol. PD AUG PY 1995 VL 269 IS 2 BP C392 EP C402 PG 11 WC Cell Biology; Physiology SC Cell Biology; Physiology GA RP469 UT WOS:A1995RP46900012 PM 7653521 ER PT J AU AJIE, HO CONNOR, MJ LEE, WNP BASSILIAN, S BERGNER, EA BYERLEY, LO AF AJIE, HO CONNOR, MJ LEE, WNP BASSILIAN, S BERGNER, EA BYERLEY, LO TI IN-VIVO STUDY OF THE BIOSYNTHESIS OF LONG-CHAIN FATTY-ACIDS USING DEUTERATED WATER SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE LONG-CHAIN FATTY ACID TURNOVER AND RECYCLING; CHAIN ELONGATION; PRIMERS OF FATTY ACID SYNTHESIS ID STEROL SYNTHESIS; STABLE ISOTOPES; TRITIATED-WATER; INVIVO AB To determine the contributions of preexisting fatty acid, de novo synthesis, and chain elongation in long-chain fatty acid (LCFA) synthesis, the synthesis of LCFAs, palmitate (16:0), stearate (18:0), arachidate (20:0), behenate (22:0), and lignocerate (24:0), in the epidermis, liver, and spinal cord was determined using deuterated water and mass isotopomer distribution analysis in hairless mice and Sprague-Dawley rats. Animals were given 4% deuterated water for 5 days or 8 wk in their drinking water. Blood was withdrawn at the end of these times for the determination of deuterium enrichment, and the animals were killed to isolate the various tissues for lipid extraction for the determination of the mass isotopomer distributions. The mass isotopomer distributions in LCFA were incompatible with synthesis from a single pool of primer. The synthesis of palmitate, stearate, arachidate, behenate, and lignocerate followed the expected biochemical pathways for the synthesis of LCFAs. On average, three deuterium atoms were incorporated for every addition of an acetyl unit. The isotopomer distribution resulting from chain elongation and de novo synthesis can be described by the linear combination of two binomial distributions. The proportions of preexisting, chain elongation, and de novo-synthesized fatty acids as a percentage of the total fatty acids were determined using multiple linear regression analysis. Fractional synthesis was found to vary, depending on the tissue type and the fatty acid, from 47 to 87%. A substantial fraction (24-40%) of the newly synthesized molecules was derived from chain elongation of unlabeled (recycled) palmitate. C1 UNIV CALIF LOS ANGELES, LOS ANGELES CTY HARBOR MED CTR, INST RES & EDUC, TORRANCE, CA 90502 USA. UNIV CALIF LOS ANGELES, SCH MED, CTR HLTH SCI, DIV DERMATOL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. UNIV TEXAS, DEPT HUMAN ECOL, AUSTIN, TX 78712 USA. FU NCRR NIH HHS [M01-RR-00425]; NIDDK NIH HHS [R01-DK-46353]; NIEHS NIH HHS [R01-ES-03597] NR 19 TC 22 Z9 22 U1 0 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD AUG PY 1995 VL 269 IS 2 BP E247 EP E252 PG 6 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA RP468 UT WOS:A1995RP46800007 PM 7653542 ER PT J AU MORROW, NS QUINONEZ, G WEINER, H TACHE, Y GARRICK, T AF MORROW, NS QUINONEZ, G WEINER, H TACHE, Y GARRICK, T TI INTERLEUKIN-1-BETA IN THE DORSAL VAGAL COMPLEX INHIBITS TRH ANALOG-INDUCED STIMULATION OF GASTRIC CONTRACTILITY SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE VAGUS; INTERLEUKIN-1 RECEPTOR ANTAGONIST; HYPOGLOSSAL NUCLEUS ID THYROTROPIN-RELEASING-HORMONE; NERVOUS-SYSTEM ACTION; RECEPTOR ANTAGONIST; ACID-SECRETION; INTESTINAL MOTILITY; BRAIN; RATS; EXPRESSION; LOCALIZATION; MECHANISMS AB The effect of murine interleukin-1 beta (mIL-1 beta) microinjected into the dorsal vagal complex (DVC) on thyrotropin-releasing hormone (TRH) analogue (RX-77368)-induced stimulation of gastric contractility was examined in fasted, urethan-anesthetized rats. Gastric corpus contractions were measured with extraluminal force transducers and analyzed by computer. Microinjection of RX-17368 (30 ng) into the right DVC with mIL-1 beta microinjected either into the right (100, 250 pg) or into the left (100, 500 pg) DVC inhibited gastric contractility for 30-120 min postinjection. Peak suppression of gastric contractility (64-78%) occurred at 50-60 min postinjection. Microinjedion of mIL-1 beta into the DVC at a lower dose (10 pg) or into sites adjacent to the DVC (100-500 pg) did not suppress the stimulated gastric contractility pattern. Injection of mIL-1 beta (250 pg) or 0.1% bovine serum albumin into the DVC alone did not alter basal gastric contractlity. Intracisternal injection of the IL-1 receptor antagonist (250 ng/10 mu l) abofished the inhibitory effect of mIL-1 beta (250 pg) on gastric contractility. These results demonstrate that mIL-1 beta acts in the DVC to inhibit vagally stimulated gastric contractility, and its action is mediated by IL-1 receptors. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT PSYCHIAT, CTR GASTROENTER BIOL, CTR ULCER RES & EDUC, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, RES DEPT, LOS ANGELES, CA USA. UNIV CALIF LOS ANGELES, DEPT PSYCHIAT & BIOBEHAV SCI, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, DEPT MED, LOS ANGELES, CA 90024 USA. FU NIADDK NIH HHS [AM-33061]; NIDDK NIH HHS [DK-41301]; NIMH NIH HHS [MH-00663] NR 40 TC 8 Z9 8 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD AUG PY 1995 VL 269 IS 2 BP G196 EP G202 PG 7 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA RP346 UT WOS:A1995RP34600003 PM 7653558 ER PT J AU RUSSELL, WJ HO, YS PARISH, G JACKSON, RM AF RUSSELL, WJ HO, YS PARISH, G JACKSON, RM TI EFFECTS OF HYPOXIA ON MNSOD EXPRESSION IN MOUSE LUNG SO AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY LA English DT Article DE SUPEROXIDE DISMUTASE; GENE REGULATION; OXYGEN TOXICITY ID CONTAINING SUPEROXIDE-DISMUTASE; ANTIOXIDANT ENZYMES; RAT LUNGS; ESCHERICHIA-COLI; MAMMALIAN-CELLS; OXYGEN; SEQUENCE; PREEXPOSURE; TOXICITY; OXIDANTS AB Mitochondrial manganese-containing SOD (MnSOD) is located at the primary site of O-2 metabolism, and its expression may be regulated by changes in O-2 level. We hypothesized that lung MnSOD expression and promoter activity would decrease in response to hypoxia. We tested effects of hypoxia (10% O-2 at sea level for 7 days) on chloramphenicol acetyltransferase (CAT) reporter and MnSOD gene expression in transgenic mice. The transgene consisted of a 3.3-kb portion of the rat MnSOD gene 5' flanking region coupled to a CAT reporter gene. Lung MnSOD activity in male (but not female) mice decreased significantly after hypoxia exposure. The decrease in MnSOD enzymatic activity in male mice was specific. Neither total SOD nor glucose-6-phosphate dehydrogenase (G-6-PDH) activity decreased significantly in hypoxia. CAT protein expression decreased in transgenic males exposed to hypoxia, while CAT protein expression in hypoxic transgenic females remained comparable with controls. The mRNA for both the native MnSOD and the MnSOD-CAT reporter genes remained constant after hypoxia, as did CuZnSOD and G-6-PDH mRNAs. C1 UNIV ALABAMA, DIV PULM & CRIT CARE MED, BIRMINGHAM, AL 35294 USA. BIRMINGHAM VET AFFAIRS MED CTR, BIRMINGHAM, AL 35294 USA. WAYNE STATE UNIV, DETROIT, MI 48202 USA. FU NHLBI NIH HHS [HL-39147, HL-39585]; NIDDK NIH HHS [1T32-DK-07545] NR 39 TC 20 Z9 20 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 1040-0605 J9 AM J PHYSIOL-LUNG C JI Am. J. Physiol.-Lung Cell. Mol. Physiol. PD AUG PY 1995 VL 269 IS 2 BP L221 EP L226 PG 6 WC Physiology; Respiratory System SC Physiology; Respiratory System GA RP719 UT WOS:A1995RP71900013 PM 7653584 ER PT J AU NGUYEN, MH BARCHIESI, F MCGOUGH, DA YU, VL RINALDI, MG AF NGUYEN, MH BARCHIESI, F MCGOUGH, DA YU, VL RINALDI, MG TI IN-VITRO EVALUATION OF COMBINATION OF FLUCONAZOLE AND FLUCYTOSINE AGAINST CRYPTOCOCCUS-NEOFORMANS VAR NEOFORMANS SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID AMPHOTERICIN-B; MENINGITIS; THERAPY; SYNERGY; AIDS AB Amphotericin B and fluconazole are current acceptable therapies for cryptococcal meningitis; however, their effect remains suboptimal. The combination of fluconazole and flucytosine has yielded encouraging clinical results in human immunodeficiency virus patients,vith cryptococcal meningitis, To investigate the biological basis of this finding, we performed in vitro combination testing of fluconazole and flucytosine against 50 clinical strains of Cryptococcus neoformans var, neoformans, Synergy (fractional inhibitory concentration index of <1.0) was observed in 62% of cases, while antagonism (fractional inhibitory concentration index of >2.0) was not observed, For cases in which synergy was not achieved (autonomous or additive effects), the beneficial effect of the combination was still seen (i.e,, there was still a decrease, although not as dramatic, in the MIC of one or both drugs when used in combination), The in vitro inhibitory action of flucytosine was greatly enhanced by the addition of fluconazole; the flucytosine MICs for Cryptococcus isolates were markedly decreased to concentrations which were severalfold lower than the achievable cerebrospinal fluid flucytosine concentration, On the other hand, the addition of flucytosine did not greatly enhance the in vitro activity of fluconazole if the initial fluconazole MIC for the isolate was greater than or equal to 8 mu g/ml. Controlled clinical studies are warranted to further elucidate the potential utility of fluconazole-flucytosine combination therapy. C1 UNIV PITTSBURGH,MED CTR,DEPT MED,DIV INFECT DIS,PITTSBURGH,PA 15213. VET AFFAIRS MED CTR,PITTSBURGH,PA. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV ANCONA,IST MALATTIE INFECT & MED PUBBL,ANCONA,ITALY. NR 14 TC 60 Z9 65 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD AUG PY 1995 VL 39 IS 8 BP 1691 EP 1695 PG 5 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RM378 UT WOS:A1995RM37800009 PM 7486902 ER PT J AU GRAYBILL, JR BOCANEGRA, R AF GRAYBILL, JR BOCANEGRA, R TI LIPOSOMAL AMPHOTERICIN-B THERAPY OF MURINE HISTOPLASMOSIS SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Note ID ACQUIRED-IMMUNODEFICIENCY-SYNDROME; ITRACONAZOLE; IMMUNITY; INVITRO; MICE AB Liposomal amphotericin B (AmBisome) was compared with amphotericin B deoxycholate for the treatment of disseminated murine histoplasmosis. Liposomal amphotericin B was well tolerated and, milligram for milligram, was as potent as amphotericin B deoxycholate. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. RP GRAYBILL, JR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,DIV INFECT DIS,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. FU PHS HHS [N0I-A1-25141] NR 19 TC 22 Z9 24 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD AUG PY 1995 VL 39 IS 8 BP 1885 EP 1887 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RM378 UT WOS:A1995RM37800048 PM 7486941 ER PT J AU SULTZER, DL MAHLER, ME CUMMINGS, JL VANGORP, WG HINKIN, CH BROWN, C AF SULTZER, DL MAHLER, ME CUMMINGS, JL VANGORP, WG HINKIN, CH BROWN, C TI CORTICAL ABNORMALITIES ASSOCIATED WITH SUBCORTICAL LESIONS IN VASCULAR DEMENTIA - CLINICAL AND POSITRON EMISSION TOMOGRAPHIC FINDINGS SO ARCHIVES OF NEUROLOGY LA English DT Article ID MULTI-INFARCT DEMENTIA; CEREBRAL BLOOD-FLOW; ALZHEIMERS-DISEASE; ENERGY-METABOLISM; THALAMIC STROKE; RATING-SCALE; DIASCHISIS; CORTEX; SEVERITY; HUMANS AB Objective: To examine the effects of subcortical lesions on cortical metabolic rate and clinical symptoms in patients with vascular dementia. Method: Eleven elderly patients with vascular dementia who demonstrated no lesion involving the cerebral cortex on magnetic resonance imaging underwent F-18-fluorodeoxyglucose positron emission tomography to assess global cortical metabolism and metabolic activity in each cortical lobe. Subcortical lesions on magnetic resonance imaging (periventricular hyperintensities, deep white matter hyperintensities, and subcortical lacunar infarcts) were measured using a graded scale of severity. Cognitive and noncognitive symptoms were assessed with the Neurobehavioral Rating Scale. Results: Reduced cortical metabolism was generally associated with the severity of subcortical pathologic changes, but there was substantial heterogeneity in the relationship between subcortical lesions and cortical metabolic activity. Mean global cortical metabolism was lower in patients with periventricular hyperintensities in anterior subcortical regions than in those without such lesions. The metabolic rate in the frontal cortex was lower in patients with a lacunar infarct of the basal ganglia or thalamus than in those without. Neurobehavioral Rating Scale total score, the Verbal Output Disturbance factor score, and the Anxiety/Depression factor score were correlated with the severity of white matter lesions. Conclusions: Cortical metabolic dysfunction is related to ischemic subcortical lesions in patients with vascular dementia. Metabolism in the frontal cortex may be particularly dependent on pathologic alterations of subcortical nuclei. Anxiety, depression, and the overall severity of neuropsychiatric symptoms in vascular dementia are associated with the extent of white matter ischemia. C1 W LOS ANGELES VET AFFAIRS MED CTR,NUCL MED SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHOL SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV 116AF,BEHAV NEUROSCI SECT,LOS ANGELES,CA 90073. FU NIA NIH HHS [AG10123]; NIMH NIH HHS [MH00910] NR 39 TC 112 Z9 112 U1 1 U2 2 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD AUG PY 1995 VL 52 IS 8 BP 773 EP 780 PG 8 WC Clinical Neurology SC Neurosciences & Neurology GA RN516 UT WOS:A1995RN51600007 PM 7639629 ER PT J AU ROODMAN, GD AF ROODMAN, GD TI OSTEOCLAST FUNCTION IN PAGETS-DISEASE AND MULTIPLE-MYELOMA SO BONE LA English DT Article; Proceedings Paper CT XII International Conference on Calcium Regulating Hormones CY FEB 14-19, 1995 CL MELBOURNE, AUSTRALIA DE PAGETS DISEASE; OSTEOCLASTS; MULTIPLE MYELOMA; INTERLEUKIN-6; BONE RESORPTION; OSTEOCLAST ULTRASTRUCTURE ID B-CELL MALIGNANCIES; NUCLEAR INCLUSIONS; BONE; MARROW; INTERLEUKIN-6; RESORPTION; CYTOKINE AB Paget's disease of bone and multiple myeloma are characterized by increased numbers of osteoclasts and markedly increased bone resorption at the sites of the disease, In Paget's disease the osteoclasts are abnormal morphologically and contain viral-like nuclear inclusions, but in multiple myeloma the osteoclasts are normal, The bone lesions in both Paget's disease and multiple myeloma appear to be due to local stimulation of osteoclast formation and bone resorption, In situ hybridization techniques, bone marrow cultures, and cytokine assays have been used to examine osteoclast function in Paget's disease and multiple myeloma, Interleukin-6 (IL-6) has been implicated as a potential mediator for the increased osteoclast activity in both diseases, In Paget's disease, IL-6 is produced by the osteoclasts, the osteoclasts express IL-6 receptors and IL-6 mRNA, and increased levels of IL-6 are present in the marrow plasma and serum of these patients, Similarly, increased levels of IL-6 have been detected in sera from some patients with multiple myeloma, Multiple myeloma cells do not produce IL-6 in vivo but marrow stromal cells or the osteoclasts may be the source of IL-6 in multiple myeloma, IL-6 is a growth factor for multiple myeloma cells, and treating patients with anti-IL-6 decreases the tumor burden in some patients, Thus, IL-6 may be an autocrine/paracrine factor in both Paget's disease and in multiple myeloma, Multiple myeloma cells also produce osteoclast activating factors (OAFs) that can stimulate osteoclast formation and activity, In vitro studies have suggested IL-1, TNF-alpha, and lymphotoxin as potential OAFs, but the identity of the OAF produced by multiple myeloma cells in vivo remains uncertain, Thus, osteoclast function is markedly enhanced in Paget's disease and multiple myeloma, but the basis for the increased osteoclast activity remains to be clearly defined. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED HEMATOL,SAN ANTONIO,TX. VET ADM MED CTR,SAN ANTONIO,TX. NR 30 TC 28 Z9 28 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 8756-3282 J9 BONE JI Bone PD AUG PY 1995 VL 17 IS 2 SU S BP S57 EP S61 DI 10.1016/8756-3282(95)00179-H PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RU724 UT WOS:A1995RU72400016 PM 8579899 ER PT J AU FILLEY, CM AF FILLEY, CM TI NEUROPSYCHIATRIC FEATURES OF LEWY BODY DISEASE SO BRAIN AND COGNITION LA English DT Article ID PARKINSONS-DISEASE; PROGRESSIVE DEMENTIA; ALZHEIMERS-DISEASE; BODIES; PATHOLOGY; TANGLES AB Although traditionally associated with Parkinson's disease, the eosinophilic intracytoplasmic neuronal inclusion known as the Lewy body has recently been regarded as the primary neuropathologic finding in a variety of conditions affecting the aging brain. The term Lewy Body Disease (LBD) will be used in this review to refer to a spectrum of clinical states varying from those due to incidental or mildly symptomatic histopathologic changes to progressive dementia and psychosis. Many unanswered questions remain about the neurobehavioral and neuropathological implications of Lewy bodies, but it is useful to consider the LED spectrum in terms of the variable effects on neuropsychiatric function that can be observed clinically. (C) 1995 Academic Press, Inc. C1 UNIV COLORADO,SCH MED,DEPT PSYCHIAT,DENVER,CO 80262. DENVER VET AFFAIRS MED CTR,DENVER,CO. RP FILLEY, CM (reprint author), UNIV COLORADO,SCH MED,HLTH SCI CTR,DEPT NEUROL,BEHAV NEUROL SECT,B-183,4200 E 9TH AVE,DENVER,CO 80262, USA. NR 31 TC 13 Z9 13 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0278-2626 J9 BRAIN COGNITION JI Brain Cogn. PD AUG PY 1995 VL 28 IS 3 BP 229 EP 239 DI 10.1006/brcg.1995.1254 PG 11 WC Neurosciences; Psychology, Experimental SC Neurosciences & Neurology; Psychology GA RT796 UT WOS:A1995RT79600003 PM 8546851 ER PT J AU CUMMINGS, JL AF CUMMINGS, JL TI LEWY BODY DISEASES WITH DEMENTIA - PATHOPHYSIOLOGY AND TREATMENT SO BRAIN AND COGNITION LA English DT Article ID ALZHEIMERS-DISEASE; PARKINSONS-DISEASE; CONTROLLED TRIAL; SENILE DEMENTIA; PROTEIN; PHYSOSTIGMINE; ANTAGONISTS; HYPOTHESIS; GLUTAMATE; TACRINE AB There are four major Lewy body disorders with dementia: Parkinson's disease (PD), PD with Alzheimer's disease (AD), cortical Lewy bodies and neuritic plaques, and cortical Lewy bodies and no concomitant AD-type pathology. A variety of pathogenetic processes may underly this panoply of diseases including oxidative stress, excitatory amino acid toxicity, amyloidogenesis, neurofibrillary tangle formation, inflammation, apoptotic cell death, and neurotransmitter deficiency. Treatment strategies include transmitter replacement, neuroprotection, agents to limit AD-type pathology, iron chelation, and anti-inflammatory drugs. Patients with Lewy body disorders exhibit cognitive decline resulting from a variety of disease processes and treatment must address the disease-specific pathogenesis. (C) 1995 Academic Press, Inc. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,BEHAV NEUROSCI SECT,LOS ANGELES,CA 90073. RP CUMMINGS, JL (reprint author), UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT NEUROL,710 WESTWOOD PLAZA,LOS ANGELES,CA 90024, USA. FU NIA NIH HHS [AG10123] NR 69 TC 14 Z9 14 U1 1 U2 7 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0278-2626 J9 BRAIN COGNITION JI Brain Cogn. PD AUG PY 1995 VL 28 IS 3 BP 266 EP 280 DI 10.1006/brcg.1995.1257 PG 15 WC Neurosciences; Psychology, Experimental SC Neurosciences & Neurology; Psychology GA RT796 UT WOS:A1995RT79600006 PM 8546854 ER PT J AU FOLLIS, F MILLER, K SCREMIN, OU PETT, S KESSLER, R TEMES, T WERNLY, JA AF FOLLIS, F MILLER, K SCREMIN, OU PETT, S KESSLER, R TEMES, T WERNLY, JA TI EXPERIMENTAL DELAYED POSTISCHEMIC SPINAL-CORD HYPOPERFUSION AFTER AORTIC CROSS-CLAMPING SO CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES LA English DT Article ID BLOOD-FLOW; DETERIORATING STROKE; ISCHEMIA; MODEL; PARAPLEGIA; DAMAGE; RABBIT; REPERFUSION; OCCLUSION; INJURY AB Background: As in the brain, recent evidence has suggested a defect in the microcirculation during the reperfusion period after spinal cord ischemia. This investigation was undertaken in order to delineate blood flow dynamics in the postischemic spinal cord of the rat. Methods: Male Sprague-Dawley rats underwent cross-clamping of the aorta and subclavian arteries (XC) for 11 minutes. Spinal cord blood flow (SCBF) was measured by autoradiography in the gray and white matter of cervical (Ce), thoracic (Th) and lumbar (Lu) regions during XC, 1 h, 6 h and 24 h (XC n = 8, 1 h n = 9, 6 h n = 9, and 24 h n = 11, groups) after XC. Control groups underwent surgical manipulations and SCBF measurement but no XC (Sham 1, n = 8), or clamping of the subclavian arteries only (Sham 2, n = 8). Results: In Ce cord, there was no difference between SCBF of 1 h, 6 h, 24 h and Sham 1. In Th cord, SCBF was reduced during XC (P < 0.003 vs. Sham 2), 1 h, 6 h (P < 0.04 and P < 0.01 vs. Sham 1). In Lu cord, SCBF was not detectable in XC, and depressed in 1 h (P < 0.003) and 6 h (P < 0.003). There was no difference between 24 h and Sham 1 in Ce, Th, and Lu cords. Conclusions: The study demonstrated a period of delayed postischemic hypoperfusion in the white and gray matter of Th and Lu cord segments lasting 6 h after XC. The phenomenon may play an important role in the ultimate fate of neural elements with borderline viability after ischemic injury. C1 UNIV CALIF LOS ANGELES, SCH MED, W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA USA. RP UNIV NEW MEXICO, DEPT CARDIOVASC & THORAC SURG, 2211 LOMAS BLVD, ALBUQUERQUE, NM 87131 USA. NR 28 TC 5 Z9 6 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0317-1671 EI 2057-0155 J9 CAN J NEUROL SCI JI Can. J. Neurol. Sci. PD AUG PY 1995 VL 22 IS 3 BP 202 EP 207 PG 6 WC Clinical Neurology SC Neurosciences & Neurology GA RQ318 UT WOS:A1995RQ31800005 PM 8529172 ER PT J AU GOETZ, MB MATHISEN, GE AF GOETZ, MB MATHISEN, GE TI CLINICAL COURSE AND TREATMENT OF ADULTS WITH SEVERE MEASLES PNEUMONITIS SO CLINICAL INFECTIOUS DISEASES LA English DT Letter ID VITAMIN-A; TRIAL C1 UNIV CALIF LOS ANGELES,OLIVE VIEW MED CTR,DEPT INFECT DIS,SYLMAR,CA. RP GOETZ, MB (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,INFECT DIS SECT,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 8 TC 0 Z9 0 U1 1 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD AUG PY 1995 VL 21 IS 2 BP 443 EP 443 PG 1 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA RM817 UT WOS:A1995RM81700032 PM 8562761 ER PT J AU JOKI, R KLEIN, RL LOPESVIRELLA, MF COLWELL, JA AF JOKI, R KLEIN, RL LOPESVIRELLA, MF COLWELL, JA TI RELEASE OF PLATELET PLASMINOGEN-ACTIVATOR INHIBITOR-1 IN WHOLE-BLOOD IS INCREASED IN PATIENTS WITH TYPE-II DIABETES SO DIABETES CARE LA English DT Article ID THROMBOLYSIS; AGGREGATION; THROMBOSIS; MELLITUS; DISEASE AB OBJECTIVE - To compare platelet plasminogen activator inhibitor 1 (PAI-1) release in type II diabetic patients and healthy control subjects. RESEARCH DESIGN AND METHODS - We studied a group of 27 diabetic patients and a group of 16 nondiabetic control subjects. Whole-blood platelet aggregation, defined as a decrease in platelet count during shaking (180 rpm) of blood samples al 37 degrees C, and plasma PAI-1 antigen concentrations were measured in parallel at time 0, 7.5, 15, 30, 60, 120, and 180 min. RESULTS - Platelet aggregation did not differ significantly between the two groups at any time period. However, the increase in plasma PAI-1 antigen concentration over basal levels at time 0 was higher for the group of diabetic patients when compared with their matched control subjects. The increment of PAI-1 antigen was 61.8 +/- 29.4 vs. 35.9 +/- 13.4 ng/ml (P < 0.005, means +/- SD) after 180 min for the diabetic and control subjects, respectively. Platelet PAI-1 release was correlated to very-low-density lipoprotein cholesterol and triglyceride plasma levels, but not to HbA(1c) levels. CONCLUSIONS - Platelets of patients with type II diabetes release significantly more PAI-1 than platelets of healthy subjects al the same level of platelet aggregation. This may contribute to enhanced thrombosis in diabetes. C1 MED UNIV S CAROLINA,DIV ENDOCRINOL DIABET & METAB,CHARLESTON,SC 29425. RP JOKI, R (reprint author), RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,109 BEE ST,CHARLESTON,SC 29401, USA. NR 21 TC 4 Z9 4 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0149-5992 J9 DIABETES CARE JI Diabetes Care PD AUG PY 1995 VL 18 IS 8 BP 1150 EP 1155 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RM113 UT WOS:A1995RM11300009 ER PT J AU UY, HL GUISE, TA DELAMATA, J TAYLOR, SD STORY, BM DALLAS, MR BOYCE, BF MUNDY, GR ROODMAN, GD AF UY, HL GUISE, TA DELAMATA, J TAYLOR, SD STORY, BM DALLAS, MR BOYCE, BF MUNDY, GR ROODMAN, GD TI EFFECTS OF PARATHYROID-HORMONE (PTH)-RELATED PROTEIN AND PTH ON OSTEOCLASTS AND OSTEOCLAST PRECURSORS IN-VIVO SO ENDOCRINOLOGY LA English DT Article ID COLONY-STIMULATING FACTOR; BONE-MARROW CULTURES; HUMORAL HYPERCALCEMIA; IMMUNORADIOMETRIC ASSAY; OSTEOTROPIC HORMONES; SYNTHETIC PEPTIDE; CELL-FORMATION; ORGAN-CULTURE; RESORPTION; MALIGNANCY AB Increased production of PTH-related protein (PTHrP) and PTH is frequently responsible for hypercalcemia and its associated morbidity. However, it is unclear whether these peptides produce identical effects on cells in the osteoclast lineage in vivo. To examine the effects of continuous in vivo exposure to these factors on both the osteoclast precursors and mature osteoclasts, we inoculated Chinese hamster ovarian cells expressing PTH-(1-84), PTHrP-(1-141), or nontransfected Chinese hamster ovarian cells into nude mice. The effects of these tumors on blood ionized calcium, plasma PTH and PTHrP concentrations, and osteoclast formation were then determined. PTH and PTHrP tumor-bearing mice became hypercalcemic (1.90 +/- 0.04 and 1.97 +/- 0.16 mmol/liter, respectively) compared with control mice (1.29 +/- 0.015 mmol/liter). After 4 days of hypercalcemia, mice were killed, and bone marrow cells were harvested to examine cells at three discrete stages of osteoclast development: multipotent osteoclast precursors, the granulocyte/macrophage colony-forming unit; more committed marrow mononuclear osteoclast precursors; and mature osteoclasts. Neither PTH nor PTHrP had an effect on granulocyte/macrophage colony-forming unit, but similarly increased the number of more committed mononuclear osteoclast progenitors as well as mature osteoclasts in the calvaria. No differences were detected between the effects of PTH and PTHrP on cells in the osteoclast lineage in vivo. Thus, PTH and PTHrP appear to affect only more differentiated cells in the osteoclast lineage, and the differences in osteoclastic bone resorption between primary hyperparathyroidism and humoral hypercalcemia of malignancy are probably due to mechanisms other than effects on osteoclast precursor cells in vivo. C1 AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV 151,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV HEMATOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. FU NCI NIH HHS [CA-40035]; NIADDK NIH HHS [AM-35188]; NIAMS NIH HHS [AR-39529] NR 37 TC 30 Z9 30 U1 0 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD AUG PY 1995 VL 136 IS 8 BP 3207 EP 3212 DI 10.1210/en.136.8.3207 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RL632 UT WOS:A1995RL63200006 PM 7628353 ER PT J AU GRAYBILL, JR BOCANEGRA, R LUTHER, M AF GRAYBILL, JR BOCANEGRA, R LUTHER, M TI ANTIFUNGAL COMBINATION THERAPY WITH GRANULOCYTE-COLONY-STIMULATING FACTOR AND FLUCONAZOLE IN EXPERIMENTAL DISSEMINATED CANDIDIASIS SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Note ID RISK-FACTORS; CSF; CANDIDEMIA; INFECTION AB The present studies were performed to determine whether any beneficial interaction results from combination therapy with recombinant human granulocyte colony-stimulating factor (GCSF) and fluconazole in disseminated candidiasis in mice. Fluconazole extended survival and reduced tissue counts. GCSF had no direct benefit, but GCSF combined with fluconazole extended survival beyond that for fluconazole alone and reduced renal tissue counts below those for fluconazole alone. Thus, GCSF and fluconazole may be a useful combined therapy for disseminated candidiasis. C1 AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,DIV INFECT DIS,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,RES DEPT,SAN ANTONIO,TX 78284. RP GRAYBILL, JR (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV INFECT DIS,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIAID NIH HHS [N01-AI-25141] NR 15 TC 27 Z9 27 U1 0 U2 0 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD AUG PY 1995 VL 14 IS 8 BP 700 EP 703 DI 10.1007/BF01690878 PG 4 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA RV705 UT WOS:A1995RV70500007 PM 8565989 ER PT J AU YONEMURA, Y KU, H HIRAYAMA, F OGAWA, M AF YONEMURA, Y KU, H HIRAYAMA, F OGAWA, M TI IL-3 OR IL-1 ABROGATES THE RECONSTITUTING ABILITY OF HEMATOPOIETIC STEM-CELLS SO EXPERIMENTAL HEMATOLOGY LA English DT Meeting Abstract C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. NR 0 TC 5 Z9 5 U1 0 U2 0 PU CARDEN JENNINGS PUBL COLTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD AUG PY 1995 VL 23 IS 8 BP 899 EP 899 PG 1 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA RP116 UT WOS:A1995RP11600549 ER PT J AU OBERLEY, TD SEMPF, JM OBERLEY, LW AF OBERLEY, TD SEMPF, JM OBERLEY, LW TI IMMUNOHISTOCHEMICAL LOCALIZATION OF ANTIOXIDANT ENZYMES DURING HAMSTER-KIDNEY DEVELOPMENT SO HISTOCHEMICAL JOURNAL LA English DT Article ID GLUTATHIONE-S-TRANSFERASE; SYRIAN-HAMSTER; SUPEROXIDE DISMUTASES; RAT; TISSUES; DIFFERENTIATION; ISOENZYMES; EPITHELIUM; SUBSTRATE; MICE AB Immunolocalization studies of hamster kidney development were performed using polyclonal antibodies to antioxidant enzymes, including antibodies to copper, zinc and manganese superoxide dismutases, catalase, glutathione peroxidase and glutathione S-transferases and their subunits. Antibodies to extracellular matrix proteins were also studied to determine the temporal sequence between expression of immunoreactive protein for basement membrane proteins, which serve as markers of embryonic induction of nephron development, and antioxidant enzyme expression in kidney development. Immunoreactive proteins for antioxidant enzymes were not detectable in the developing kidney until after extracellular matrix proteins had been deposited. However, immunoreactive proteins for the antioxidant enzymes copper, zinc and manganese superoxide dismutases, catalase, and cw class glutathione S-transferase Ya subunit were detected in renal tubules before birth. mu, class glutathione S-transferase subunits Yb-1 and Yb-2 stained transitional epithelium at high levels before birth. Our results indicate: (1) each type of kidney cell has a unique antioxidant enzyme profile, (2) antioxidant enzymes are expressed in different types of cell at different times during development, but antioxidant enzyme immunoreactive protein was not present until after immunoreactive proteins for extracellular matrix molecules were detected, and (3) certain antioxidant enzymes are present before birth, indicating that high oxygen tension present at birth is not crucial for induction of immunoreactive protein. C1 UNIV WISCONSIN,SCH MED,DEPT PATHOL,MADISON,WI 53705. UNIV IOWA,COLL MED,RADIAT RES LAB,IOWA CITY,IA 52242. RP OBERLEY, TD (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL SERV,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU PHS HHS [41267] NR 27 TC 8 Z9 8 U1 0 U2 1 PU CHAPMAN HALL LTD PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8HN SN 0018-2214 J9 HISTOCHEM J JI Histochem.J. PD AUG PY 1995 VL 27 IS 8 BP 575 EP 586 PG 12 WC Cell Biology SC Cell Biology GA RP232 UT WOS:A1995RP23200002 PM 8550376 ER PT J AU INGALLS, RR RICE, PA QURESHI, N TAKAYAMA, K LIN, JS GOLENBOCK, DT AF INGALLS, RR RICE, PA QURESHI, N TAKAYAMA, K LIN, JS GOLENBOCK, DT TI THE INFLAMMATORY CYTOKINE RESPONSE TO CHLAMYDIA-TRACHOMATIS INFECTION IS ENDOTOXIN MEDIATED SO INFECTION AND IMMUNITY LA English DT Article ID OUTER-MEMBRANE PROTEIN; DIPHOSPHORYL-LIPID-A; PROTECTIVE MONOCLONAL-ANTIBODIES; HUMAN-IMMUNODEFICIENCY-VIRUS; RHODOPSEUDOMONAS-SPHAEROIDES; LIPOPOLYSACCHARIDE EPITOPE; ESCHERICHIA-COLI; HUMAN-MONOCYTES; EXPRESSION; CELLS AB Chlamydia trachomatis is a major etiologic agent of sexually transmitted diseases. Although C. trachomatis is a gram-negative pathogen, chlamydial infections are not generally thought of as endotoxin-mediated diseases. A molecular characterization of the acute immune response to chlamydia, especially with regard to the role of its lipopolysaccharide (LPS), remains to be undertaken. We extracted 15 mg of LPS from 5 x 10(12) C. trachomatis elementary bodies (EB) for analysis of structure and biological activity. When methylated lipid A was subjected to high-pressure liquid chromatography followed by mass spectrometry, the majority of the lipid A was found to be pentaacyl. The endotoxin activities of whole C. trachomatis EB and purified LPS were characterized in comparison with whole Salmonella minnesota R595 and with S. minnesota R595 LPS and lipooligosaccharide from Neisseria gonorrhoeae. Both C. trachomatis LPS and whole EB induced the release of tumor necrosis factor alpha from whole blood ex vivo, and C. trachomatis LPS was capable of inducing the translocation of nuclear factor kappa B in a Chinese hamster ovary fibroblast cell line transfected with the LPS receptor CD14. In both assays, however, C. trachomatis was similar to 100-fold less potent than S. minnesota and N. gonorrhoeae. The observation that C. trachomatis is a weak inducer of the inflammatory cytokine response correlates with the clinical observation that, unlike N. gonorrhoeae infection, genital tract infection with C. trachomatis is often asymptomatic. The ability of specific LPS antagonists to completely inhibit the tumor necrosis factor alpha-inducing activity of whole C. trachomatis EB suggests that the inflammatory cytokine response to chlamydia infection may be mediated primarily through LPS. This implies that the role of other surface protein antigens, at least in terms of eliciting the proinflammatory cytokine response, is likely to be minor. C1 BOSTON CITY HOSP,MAXWELL FINLAND LAB INFECT DIS,BOSTON,MA 02118. BOSTON UNIV,SCH MED,BOSTON,MA 02118. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. FU NIAID NIH HHS [AI33087]; NIGMS NIH HHS [GM47127]; PHS HHS [GN50870] NR 53 TC 133 Z9 136 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 1995 VL 63 IS 8 BP 3125 EP 3130 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA RK640 UT WOS:A1995RK64000047 PM 7542638 ER PT J AU SHANG, ES EXNER, MM SUMMERS, TA MARTINICH, C CHAMPION, CI HANCOCK, REW HAAKE, DA AF SHANG, ES EXNER, MM SUMMERS, TA MARTINICH, C CHAMPION, CI HANCOCK, REW HAAKE, DA TI THE RARE OUTER-MEMBRANE PROTEIN, OMPL1, OF PATHOGENIC LEPTOSPIRA SPECIES IS A HEAT-MODIFIABLE PORIN SO INFECTION AND IMMUNITY LA English DT Article ID INFLUENZAE TYPE-B; NONTYPEABLE HEMOPHILUS-INFLUENZAE; VIRULENT TREPONEMA-PALLIDUM; HARDJO-BOVIS INFECTION; ESCHERICHIA-COLI K-12; PSEUDOMONAS-AERUGINOSA; MOLECULAR-CLONING; NEISSERIA-GONORRHOEAE; MONOCLONAL-ANTIBODIES; RECOMBINANT PORIN AB The outer membranes of invasive spirochetes contain unusually small amounts of transmembrane proteins. Pathogenic Leptospira species produce a rare 31-kDa surface protein, OmpL1, which has a deduced amino acid sequence predictive of multiple transmembrane beta-strands. Studies were conducted to characterize the structure and function of this protein. Alkali, high-salt, and urea fractionation of leptospiral membranes demonstrated that OmpL1 is an integral membrane protein. The electrophoretic mobility of monomeric OmpL1 was modifiable by heat and reduction; complete denaturation of OmpL1 required prolonged boiling in sodium dodecyl sulfate (SDS), 8 M urea, and 2-mercaptoethanol. When solubilized in SDS at low temperature, a small proportion of OmpL1 exhibited an apparent molecular mass of approximately 90 kDa, indicating the existence of an SDS-unstable oligomer. OmpL1 dimers and trimers were demonstrated by nearest neighbor chemical cross-linking. In order to generate purified protein for functional studies, the ompL1 gene was ligated into the pMMB66 expression plasmid under control of the tac promoter. Although expression in Escherichia call was toxic, most of the OmpL1 produced was found in the outer membrane, as determined by subcellular fractionation. Purified recombinant OmpL1 was reconstituted into planar lipid bilayers, demonstrating an average single channel conductance of 1.1 nS, similar to the major porin activity of native leptospiral membranes. These findings indicate that OmpL1 spans the leptospiral outer membrane and functions as a porin. C1 W LOS ANGELES VET AFFAIRS MED CTR,DIV INFECT DIS 111F,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90024. UNIV BRITISH COLUMBIA,DEPT MICROBIOL,VANCOUVER,BC V6T 1Z3,CANADA. RI Hancock, Robert/E-1145-2013 FU NIAID NIH HHS [2-T32-AI07323-06] NR 58 TC 66 Z9 78 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD AUG PY 1995 VL 63 IS 8 BP 3174 EP 3181 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA RK640 UT WOS:A1995RK64000054 PM 7622245 ER PT J AU RUBENSTEIN, LV FINK, A YANO, EM SIMON, B CHERNOF, B ROBBINS, AS AF RUBENSTEIN, LV FINK, A YANO, EM SIMON, B CHERNOF, B ROBBINS, AS TI PERFORMANCE IMPROVEMENT IN HEALTH-CARE ORGANIZATIONS - INCREASING THE IMPACT OF QUALITY IMPROVEMENT ON HEALTH - AN EXPERT PANEL METHOD FOR SETTING INSTITUTIONAL PRIORITIES SO JOINT COMMISSION JOURNAL ON QUALITY IMPROVEMENT LA English DT Article ID CONSENSUS AB Background: Successful implementation of modern ongoing quality improvement (QI) methods requires investment of institutional resources, but can produce significant improvements in medical care. A health care organization's goals and objectives for improving care are expressed in strategic plan documents, which could provide a framework for planning quality improvement initiatives. However, institutional strategic planning processes are often not well linked to QI staff and resources. We developed the Quality Action Program (QAP) to connect QI to strategic planning. History: In 1991, Sepulveda VHAMC implemented a major primary care initiative, documented in a comprehensive strategic plan. The QAP was developed to enable the initiative to be evaluated within a QI context. Three-round expert panel process: To carry out the QAP, members of an institution's quality council engage in a structured consensus process. The first round involves reading educational materials and filling out a quality action survey the second round includes participation in an expert panel meeting, and the third round involves making final priority rankings. Eight-step QAP implementation plan: QI staff carry out activities to prepare for and carry out the three-round expert panel process. Results: QAP induced significant institutional QI activity directed toward achieving the top-ranked QI criterion-ensuring continuity of care. Continuity of care improved significantly over time between the pre- and post-QAP periods. Conclusions: Expert panel methods can be used to link strategic plan goals and objectives to QI efforts. C1 UNIV CALIF LOS ANGELES,LOS ANGELES,CA. RAND CORP,SANTA MONICA,CA. OLIVE VIEW UCLA MED CTR,SYLMAR,CA 91342. RALPH H JOHNSON VET AFFAIRS MED CTR,CHARLESTON,SC 29403. MED UNIV S CAROLINA,CHARLESTON,SC 29425. RP RUBENSTEIN, LV (reprint author), VET AFFAIRS MED CTR,CTR STUDY HEALTHCARE PROVIDER BEHAV,SEPULVEDA,CA 91343, USA. NR 29 TC 21 Z9 21 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1070-3241 J9 JOINT COMM J QUAL IM JI Jt. Comm. J. Qual. Improv. PD AUG PY 1995 VL 21 IS 8 BP 420 EP 432 PG 13 WC Health Policy & Services SC Health Care Sciences & Services GA RR469 UT WOS:A1995RR46900005 PM 7496455 ER PT J AU ABBOUD, SL ALSINA, M MUNDY, GR ROODMAN, GD AF ABBOUD, SL ALSINA, M MUNDY, GR ROODMAN, GD TI POTENTIAL ROLE OF INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS (IGFBPS) IN THE UNCOUPLING OF BONE TURNOVER IN MULTIPLE MYELMOMA (MM) SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S315 EP S315 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400706 ER PT J AU ALSINA, M DEVLIN, R ROODMAN, GD AF ALSINA, M DEVLIN, R ROODMAN, GD TI MECHANISMS OF OSTEOLYTIC BONE DESTRUCTION IN MULTIPLE-MYELOMA IN-VIVO SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78284 USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S503 EP S503 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48401455 ER PT J AU DEVLIN, RD REDDY, SV ROODMAN, GD AF DEVLIN, RD REDDY, SV ROODMAN, GD TI ANNEXIN-II INCREASES OSTEOCLAST (OCL) FORMATION BY STIMULATING THE PROLIFERATION OF OSTEOCLAST PRECURSORS IN HUMAN MARROW CULTURES SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S323 EP S323 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400737 ER PT J AU KATZ, MS SONG, M HYMER, TK ELANGO, N AF KATZ, MS SONG, M HYMER, TK ELANGO, N TI CYTOKINES REGULATE THE EXPRESSION OF PARATHYROID-HORMONE PARATHYROID HORMONE-RELATED PROTEIN-RECEPTOR MESSENGER-RNA IN OSTEOBLAST-LIKE CELLS SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. VET ADM MED CTR,GRECC,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S482 EP S482 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48401372 ER PT J AU REDDY, SV DEVLIN, R ROODMAN, GD AF REDDY, SV DEVLIN, R ROODMAN, GD TI CLONING AND CHARACTERIZATION OF A NOVEL AUTOCRINE OSTEOCLAST (OCL) STIMULATING FACTOR (OSF) SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S325 EP S325 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400747 ER PT J AU REDDY, SV SINGER, FR MALLETTE, L ROODMAN, GD AF REDDY, SV SINGER, FR MALLETTE, L ROODMAN, GD TI DETECTION OF MEASLES-VIRUS (MV) TRANSCRIPTS IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS AND MARROW MONONUCLEAR-CELLS FROM PATIENTS WITH PAGETS-DISEASE SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS SAN ANTONIO, SAN ANTONIO, TX 78285 USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. JOHN WAYNE CANC INST, SANTA MONICA, CA 90404 USA. ST JOHNS HOSP, SANTA MONICA, CA 90404 USA. VET ADM MED CTR, HOUSTON, TX 77030 USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0884-0431 EI 1523-4681 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S154 EP S154 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400061 ER PT J AU UY, HL MUNDY, GR DELAMATA, J BOYCE, BF GUISE, TA ROODMAN, GD AF UY, HL MUNDY, GR DELAMATA, J BOYCE, BF GUISE, TA ROODMAN, GD TI TUMOR-NECROSIS-FACTOR (TNF) ENHANCES INTERLEUKIN-6 (IL-6) MEDIATED HYPERCALCEMIA IN-VIVO SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S325 EP S325 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400744 ER PT J AU WYATT, LE YAMAGUCHI, DT LIU, Y MA, D RUDKIN, GH ISHIDA, K MILLER, TA AF WYATT, LE YAMAGUCHI, DT LIU, Y MA, D RUDKIN, GH ISHIDA, K MILLER, TA TI EXPRESSION OF THE GAP JUNCTION STRUCTURAL PROTEIN CONNEXIN-40 AND MESSENGER-RNA IN MC3T3-E1 CELLS TREATED WITH TRANSFORMING GROWTH-FACTOR-BETA AND BONE MORPHOGENETIC PROTEIN-2 SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VAMC,PLAST SURG SECT,LOS ANGELES,CA. W LOS ANGELES VAMC,GRECC,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD AUG PY 1995 VL 10 SU 1 BP S314 EP S314 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN484 UT WOS:A1995RN48400701 ER PT J AU ABBOUD, SL AF ABBOUD, SL TI REGULATION OF PLATELET-DERIVED GROWTH-FACTOR-A AND GROWTH-FACTOR-B CHAIN GENE-EXPRESSION IN BONE-MARROW STROMAL CELLS SO JOURNAL OF CELLULAR PHYSIOLOGY LA English DT Article ID PROTEIN-KINASE-C; COLONY-STIMULATING FACTOR; MICROVASCULAR ENDOTHELIAL-CELLS; TUMOR NECROSIS FACTOR; FACTOR-BETA; TGF-BETA; TRANSCRIPTIONAL REGULATION; HEMATOPOIETIC PROGENITORS; SIGNAL TRANSDUCTION; LEUKEMIC-CELLS AB MBA-2, bone marrow-derived endothelial stromal cells, express platelet-derived growth factor (PDGF) A and and chain mRNAs and secrete PDGF activity that is induced by TGF-beta. Either chain of the PDGF molecule could modulate hematopoiesis and stromal cell growth. Intracellular pathways that regulate PDGF expression in the marrow microenvironment are unknown. in the present study, we examined the mechanisms that mediate PDGF A and B chain mRNA induction by TGF-beta and the role of protein kinase C (PKC) and cyclic AMP in PDGF regulation. TGF-beta was tested in parallel with PMA, an activator of phorbol ester-dependent PKC isoforms. Both PMA (10(-7) M) and TGF-beta (2.5 ng/ml) increased PDGF A and B chain mRNA levels. The serine/threonine protein kinase inhibitor, H7, blocked PDGF A and B chain mRNA induction in response to TGF-beta. However, down-regulation of PKC by prolonged incubation with PMA failed to abolish TGF-beta induction of PDGF A and B chain mRNAs. These findings indicate that induction of PDGF A and B chain mRNAs can be mediated via phorbol ester-dependent PKC pathway. In contrast, H7-sensitive protein kinase(s) other than phorbol ester-sensitive protein kinase C mediate the effect of TGF-beta. Agents that increase cAMP were also tested for their effect on PDGF gene expression. TGF-beta-mediated induction of PDGF A and B chain mRNAs was markedly inhibited by cAMP. cAMP also blocked stimulation of PDGF A chain mRNA by PMA. The positive and negative signaling mechanisms involved in modulating PDGF in the microenvironment may be important for determining hematopoietic and stromal cell responses in vivo. (C) 1995 Wiley-Liss, Inc. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP ABBOUD, SL (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NIAMS NIH HHS [AR42306] NR 41 TC 8 Z9 8 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9541 J9 J CELL PHYSIOL JI J. Cell. Physiol. PD AUG PY 1995 VL 164 IS 2 BP 434 EP 440 DI 10.1002/jcp.1041640224 PG 7 WC Cell Biology; Physiology SC Cell Biology; Physiology GA RK702 UT WOS:A1995RK70200023 PM 7622589 ER PT J AU WRIGHT, NM RENAULT, J WILLI, S VELDHUIS, JD PANDEY, JP GORDON, L KEY, LL BELL, NH AF WRIGHT, NM RENAULT, J WILLI, S VELDHUIS, JD PANDEY, JP GORDON, L KEY, LL BELL, NH TI GREATER SECRETION OF GROWTH-HORMONE IN BLACK THAN IN WHITE MEN - POSSIBLE FACTOR IN GREATER BONE-MINERAL DENSITY - A CLINICAL RESEARCH-CENTER STUDY SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID OSTEOBLAST-LIKE CELLS; FACTOR-I; PREPUBERTAL GIRLS; TURNERS SYNDROME; BINDING PROTEIN; BODY HABITUS; SHORT-TERM; IGF-I; ESTRADIOL; WOMEN AB To determine why blacks have a higher bone mineral density (BMD) and lower incidence of osteoporosis and fractures than whites, we investigated whether the secretion of GH is higher in black than in white men. Measurements of GH were obtained at 20-min intervals over 24 h and analyzed by deconvolution. BMD was determined by dual energy x-ray absorptiometry in 16 normal black and 17 normal white men, aged 20-40 yr. The 24-h integrated GH concentration (942 +/- 174 us. 602 +/- 104 mu g/L; P = 0.0495) and GH secretory burst amplitude (0.499 +/- 0.163 vs. 0.169 +/- 0.027 mu g/L . min; P = 0.0482) were higher in black than in white men. GH burst frequency, half-duration, mass, and half-life were not different in the 2 groups. The serum 17 beta-estradiol level (162 +/- 12 vs. 108 +/- 11 pmol/L; P = 0.0011) was higher, and the serum insulin-like growth factor-binding protein 3 level (2.2 +/- 0.1 vs. 2.8 +/- 0.1 mu g/mL; P = 0.0001) was lower in black than in white men. BMD values for total body (1.22 +/- 0.02 us. 1.14 +/- 0.02 g/cm(2); P = 0.0041), forearm (0.69 +/- 0.01 vs. 0.66 +/- 0.01 g/cm(2); P = 0.0211), trochanter(0.91 +/- 0.03 vs. 0.77 +/- 0.03 g/cm(2); P = 0.0003), and femoral neck (1.08 +/- 0.03 os. 0.93 +/- 0.03 g/cm(2); P = 0.0007) were higher in black than in white men. Thus, serum 17 beta-estradiol level, GH secretion, and BMD values for the total body, forearm, trochanter, and femoral neck are greater in black than in white men. As estrogen is known to increase GH secretion and GH to increase bone mass, increases in circulating 17 beta-estradiol may contribute to the higher GH secretion and bone mass in black men. C1 MED UNIV S CAROLINA, DEPT MICROBIOL & IMMUNOL, CHARLESTON, SC 29425 USA. MED UNIV S CAROLINA, DEPT RADIOL, CHARLESTON, SC 29425 USA. MED UNIV S CAROLINA, DEPT MED & PHARMACOL, CHARLESTON, SC 29425 USA. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, CHARLESTON, SC 29425 USA. UNIV VIRGINIA, SCH MED, DEPT INTERNAL MED, CHARLOTTESVILLE, VA 22908 USA. RP WRIGHT, NM (reprint author), MED UNIV S CAROLINA, DEPT PEDIAT, 171 ASHLEY AVE, CHARLESTON, SC 29425 USA. FU NCRR NIH HHS [MO1-RR-01070]; NIAMS NIH HHS [R01-AR-36066] NR 52 TC 79 Z9 80 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1995 VL 80 IS 8 BP 2291 EP 2297 DI 10.1210/jc.80.8.2291 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN312 UT WOS:A1995RN31200005 PM 7543111 ER PT J AU HARDIN, DS AZZARELLI, B EDWARDS, J WIGGLESWORTH, J MAIANU, L BRECHTEL, G JOHNSON, A BARON, A GARVEY, WT AF HARDIN, DS AZZARELLI, B EDWARDS, J WIGGLESWORTH, J MAIANU, L BRECHTEL, G JOHNSON, A BARON, A GARVEY, WT TI MECHANISMS OF ENHANCED INSULIN SENSITIVITY IN ENDURANCE-TRAINED ATHLETES - EFFECTS ON BLOOD-FLOW AND DIFFERENTIAL EXPRESSION OF GLUT-4 IN SKELETAL-MUSCLES SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID GLUCOSE-UPTAKE; FIBER TYPES; RAT; EXERCISE; RESISTANCE; INVIVO; RESPONSIVENESS; TRANSPORTERS; SECRETION; DISPOSAL AB Exercise is associated with increased insulin sensitivity. To better understand mechanisms that could be responsible for this association, we studied seven controls and seven endurance-trained athletes. A 600 mu/m(2)-min hyperinsulinemic euglycemic glucose clamp with the limb balance technique assessed insulin sensitivity as whole body glucose uptake (WBGU) and leg glucose uptake (LGU). Indirect calorimetry and hemodynamic measurements, such as leg blood flow (LBF) and cardiac output, were performed at baseline and maximal insulin stimulation. The content of the glucose transporter GLUT 4 and muscle fiber type were evaluated in three muscle groups: vastus lateralis, gastrocnemius, and biceps. Athletes exhibited 35% higher WBGU and 30% higher LGU than controls. Basal LBF (liters per min) was higher in athletes, but the difference was not statistically significant. After insulin stimulation, LBF was 31% higher in athletes than controls (P = 0.05). Indirect calorimetry revealed that athletes had a 44% higher rate of nonoxidative glucose metabolism than controls (P = 0.01). GLUT 4 levels in vastus were 90% (P < 0.05) greater in athletes, whereas smaller differences were noted between athletes and controls in biceps and gastrocnemius. Importantly, the vastus lateralis GLUT 4 content was correlated with WBGU (r = 0.60; P < 0.05) and LGU (r = 0.62; P < 0.05). Relative numbers of oxidative fibers were increased in vastus from athletes and were positively correlated with maximal oxygen consumption (VO2 max), but GLUT 4 content could not be correlated with oxidative fiber content in individual controls or athletes. We conclude that in humans 1) endurance training enhances insulin's ability to increase LBF; 2) GLUT 4 is differentially expressed as a function of muscle group and is up-regulated by exercise in a muscle-specific manner; 3) in vastus lateralis, GLUT 4 levels are well correlated with insulin-stimulated rates of both WBGU and LGU; and 4) GLUT 4 content and in vivo insulin sensitivity do not vary as a function of fiber type composition. Thus, blood flow and GLUT 4 expression in muscle are important mechanisms that mediate greater insulin sensitivity in athletes. C1 INDIANA UNIV, SCH MED, DEPT MED, INDIANAPOLIS, IN 46202 USA. INDIANA UNIV, SCH MED, DEPT PATHOL, INDIANAPOLIS, IN 46202 USA. INDIANA UNIV, SCH MED, DEPT KINESIOL, INDIANAPOLIS, IN 46202 USA. INDIANA UNIV, SCH MED, NATL INST FITNESS & SPORT, INDIANAPOLIS, IN 46202 USA. RICHARD L ROUDEBUSH VET AFFAIRS MED CTR, INDIANAPOLIS, IN 46202 USA. MED UNIV S CAROLINA, DEPT MED, CHARLESTON, SC 29425 USA. RALPH H JOHNSON VET AFFAIRS MED CTR, CHARLESTON, SC 29425 USA. RP HARDIN, DS (reprint author), UNIV TEXAS, SCH MED, DEPT PEDIAT, 6431 FANNIN MED SCI BLDG 3122, HOUSTON, TX 77030 USA. FU NCRR NIH HHS [M01-RR-750]; NIDDK NIH HHS [DK-38765, DK-42469] NR 50 TC 80 Z9 85 U1 1 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD AUG PY 1995 VL 80 IS 8 BP 2437 EP 2446 DI 10.1210/jc.80.8.2437 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RN312 UT WOS:A1995RN31200026 PM 7629239 ER PT J AU MEREDITH, M RABAGLIA, ME METZ, SA AF MEREDITH, M RABAGLIA, ME METZ, SA TI EVIDENCE OF A ROLE FOR GTP IN THE POTENTIATION OF CA2+-INDUCED INSULIN-SECRETION BY GLUCOSE IN INTACT RAT ISLETS SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE GUANINE NUCLEOTIDES; ADENINE NUCLEOTIDES; STIMULUS-SECRETION COUPLING; DIAZOXIDE; SUCCINIC ACID ID PANCREATIC BETA-CELLS; SENSITIVE K+ CHANNELS; MOUSE B-CELLS; RELEASE; DIAZOXIDE; MECHANISMS; INHIBITION; ACID; DEHYDROGENASE; MITOCHONDRIA AB Glucose initiates insulin secretion by closing K+-ATP channels, leading to Ca2+ influx (E(1)); it also potentiates Ca2+-induced secretion (E(2)) when the K+-ATP channel is kept open using diazoxide and depolarizing concentrations of K+ are provided, To examine the roles of purine nucleotides in E(2), we compared the effects of glucose to those of the mitochondrial fuel monomethylsuccinate. Either agonist could induce E(2) accompanied by significant increases in ATP, ATP/ADP ratio, and GTP/GDP ratio; GTP increased significantly only with glucose, Mycophenolic acid (MPA), an inhibitor of cytosolic GTP synthesis, markedly inhibited glucose-induced E(2) (either in perifusions or in static incubations) and decreased GTP and the GTP/GDP ratio, but did not alter the ATP/ADP ratio, Provision of guanine (but not adenine) reversed these changes pari passu, In contrast, MPA had no effect on succinate-induced E(2), despite generally similar changes in nucleotides, A similar lack of effect of MPA on E(2) was Seen with a second mitochondrial fuel, alpha-ketoisocaproic acid (KIC), However, in the absence of diazoxide and K+, MPA blunted the secretory effects of either glucose, succinate, or KIC, These studies suggest that GTP plays a role in both glucose and succinate or KIC-induced insulin secretion at a step dependent on mitochondrial metabolism and the K+-ATP channel, In addition to mitochondrial effects, glucose appears to have extramitochondrial effects important to its potentiation of Ca2+-induced insulin secretion that are also dependent on GTP. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI 53792. UNIV WISCONSIN,DIV ENDOCRINOL,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM HOSP,MADISON,WI 53792. FU NIDDK NIH HHS [DK 37312] NR 41 TC 43 Z9 43 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD AUG PY 1995 VL 96 IS 2 BP 811 EP 821 DI 10.1172/JCI118127 PG 11 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA RM466 UT WOS:A1995RM46600021 PM 7635976 ER PT J AU BERSOHN, MM AF BERSOHN, MM TI SODIUM-PUMP INHIBITION IN SARCOLEMMA FROM ISCHEMIC HEARTS SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Article DE RABBIT HEART; SARCOLEMMAL VESICLES; SODIUM POTASSIUM ATPASE; OUABAIN; SODIUM CALCIUM EXCHANGE; PERMEABILITY ID NA+-K+-ATPASE; CALCIUM EXCHANGE; REPERFUSION; HYPOXIA; FAILURE AB Ischemic myocardial cells lose K+ and accumulate Na+. The role of the Na+/K+-pump in these changes was investigated by measuring both Na+/K+-ATPase activity and Na+ pumping in highly purified sarcolemmal vesicles from rabbit hearts made globally ischaemic for 1 h compared to non-ischemic controls, Purification of the sarcolemma was similar for control, 31 +/- 8-fold, and ischemia, 38 +/- 10-fold. The fraction of intact inside-out vesicles, in which Na+ pumping could be measured, was also the same for control, 60 +/- 16%, and ischemic, 56 +/- 8% as measured by H-3-ouabain binding in the presence and absence of detergent, Scatchard analysis of ouabain binding revealed a 26% increase in binding sites in ischemia compared to control. The Na+/K+-ATPase in the inside-out vesicles, measured as monensin-stimulated activity, was not affected by ischemia: 22 +/- 9 v 21 +/- 9 mu mol P-1 mg(-1) h(-1) for control and ischemic respectively, However, the initial velocity of ATP-dependent Na+ pumping into inside-out vesicles, assayed by subsequent exchange of Na-i(+) for Ca-45(2+) by the Na+-Ca2+ exchanger present in the vesicles, was inhibited in ischemia. At 18 mM Na-0(+) the velocity for control vesicles was 2.4 +/- 0.2 nmol mg(-1) s(-1) compared to 1.1 +/- 0.1 for ischemia vesicles, Passive sarcolemmal Na+ permeability was unchanged after 1 h of ischemia. The large reduction in Na+ pumping with unchanged Na+/K+-ATPase suggests uncoupling of the Na+/K+-pump in ischemia and a decreased ability to extrude Na+ despite the increase in number of pump sites in the sarcolemma. (C) 1995 academic Press Limited C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90073 USA. RP BERSOHN, MM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, CARDIOL SECT 111E, 11301 WILSHIRE BLVD, LOS ANGELES, CA 90073 USA. NR 22 TC 19 Z9 19 U1 1 U2 1 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD AUG PY 1995 VL 27 IS 8 BP 1483 EP 1489 DI 10.1016/S0022-2828(95)90161-2 PG 7 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA RR075 UT WOS:A1995RR07500003 PM 8523412 ER PT J AU STEVENSON, WG SAGER, PT NATTERSON, PD SAXON, LA MIDDLEKAUFF, HR WIENER, I AF STEVENSON, WG SAGER, PT NATTERSON, PD SAXON, LA MIDDLEKAUFF, HR WIENER, I TI RELATION OF PACE MAPPING QRS CONFIGURATION AND CONDUCTION DELAY TO VENTRICULAR-TACHYCARDIA REENTRY CIRCUITS IN HUMAN INFARCT SCARS SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID RADIOFREQUENCY CATHETER ABLATION; PRIOR MYOCARDIAL-INFARCTION; SLOW CONDUCTION; HEART-DISEASE; ENTRAINMENT; ACTIVATION; ORIGIN; SITE; LOCALIZATION; MECHANISMS AB Objectives. This study sought to determine the relation of the paced QRS configuration and conduction delay during pace mapping to reentry circuit sites in patients,vith ventricular tachycardia late after myocardial infarction. Background. The QRS configuration produced by ventricular pacing during sinus rhythm (pace mapping) can locate focal idiopathic ventricular tachycardias during catheter mapping, but postinfarction reentry circuits may be relatively large and contain regions of slow conduction. We hypothesized that for postinfarction ventricular tachycardia, 1) pacing during sinus rhythm at reentry circuit sites distant from the exit from the scar would produce a QRS configuration different from the tachycardia; and 2) a stimulus to QRS delay during pace mapping may be a useful guide to reentry circuit slow conduction zones. Methods. Catheter mapping and ablation were performed in 18 consecutive patients with ventricular tachycardia after myocardial infarction. At 85 endocardial sites in 13 patients, 12-lead electrocardiograms (ECGs) were recorded during pace mapping, and participation of each site in a reentry circuit was then evaluated by entrainment techniques during induced ventricular tachycardia or by application of radiofrequency current. Results. Pace maps resembled tachycardia at <30% of likely reentry circuit sites identified by entrainment criteria and at only 1 (9%) of 11 sites where radiofrequency current terminated tachycardia. Analysis of the stimulus to QRS interval during entrainment with concealed fusion shelved that the conduction time from the pacing site to the exit from the scar was longer at sites where the pace map did not resemble tachycardia. Evidence of slow conduction during pace mapping, with a stimulus to QRS interval >40 ms was observed at greater than or equal to 70% of reentry circuit sites. Conclusions. At many sites in postinfarction ventricular reentry circuits, the QRS configuration during pace mapping does not resemble the ventricular tachycardia QRS complex, consistent with relatively large reentry circuits or regions of functional conduction block during ventricular tachycardia. A stimulus to QRS delay during pace mapping is consistent with slow conduction and may aid in targeting endocardial sites for further evaluation during tachycardia. C1 HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DIV CARDIOVASC,BOSTON,MA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED & MED CTR,DIV CARDIOL,LOS ANGELES,CA. NR 29 TC 93 Z9 94 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD AUG PY 1995 VL 26 IS 2 BP 481 EP 488 DI 10.1016/0735-1097(95)80026-D PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA RM094 UT WOS:A1995RM09400027 PM 7608454 ER PT J AU DRINKA, PJ JOCHEN, AL CUISINIER, M BLOOM, R RUDMAN, I RUDMAN, D AF DRINKA, PJ JOCHEN, AL CUISINIER, M BLOOM, R RUDMAN, I RUDMAN, D TI POLYCYTHEMIA AS A COMPLICATION OF TESTOSTERONE REPLACEMENT THERAPY IN NURSING-HOME MEN WITH LOW TESTOSTERONE LEVELS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Note ID OBSTRUCTIVE SLEEP-APNEA; HYPOGONADAL MEN C1 UNIV WISCONSIN,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. VET ADM MED CTR,MILWAUKEE,WI 53295. MED COLL WISCONSIN,MILWAUKEE,WI. RP DRINKA, PJ (reprint author), WISCONSIN VET HOME,KING,WI 54946, USA. FU PHS HHS [V01-A610383] NR 12 TC 63 Z9 66 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD AUG PY 1995 VL 43 IS 8 BP 899 EP 901 PG 3 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RM829 UT WOS:A1995RM82900010 PM 7636099 ER PT J AU SHEKELLE, PG MARKOVICH, M LOUIE, R AF SHEKELLE, PG MARKOVICH, M LOUIE, R TI FACTORS ASSOCIATED WITH CHOOSING A CHIROPRACTOR FOR EPISODES OF BACK PAIN CARE SO MEDICAL CARE LA English DT Article DE CHIROPRACTIC; BACK PAIN ID FAMILY PHYSICIANS; HEALTH; COST AB Back pain is a common illness and chiropractors provide a large proportion of back pain care in the United States. This is the first study to systematically compare chiropractic patients with those who saw other providers for back pain. The authors analyzed data from the RAND Health Insurance Experiment, a community-based study of the use of health services. insurance claims forms were examined for all visits specified by the patient as occurring for back pain. Visits were grouped into episodes using decision rules and clinical judgment. The primary provider of back pain care was defined as the provider who delivered most of the services. Sociodemographic and health status and attitudes variables of patients were examined for association with the choice of chiropractor. Multivariate logistic regression models were constructed to calculate adjusted odds ratios for independent predictors. There were 1020 episodes of back pain care made by 686 different persons and encompassing 8825 visits. Results indicated that chiropractors were the primary provider for 40% of episodes, and retained as primary provider a greater percentage of their patients (92%) who had a second episode of back pain care than did medical doctors. Health insurance experiment site, white race, male sex, and high school education were independent predictors of choosing a chiropractor. Conclusions suggested that chiropractors were the choice of one third of all patients who sought back pain care, and provided care for 40% of all episodes of care. Geographic site, education, gender, and income were independent patient factors;predicting chiropractic use. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RAND CORP,GRAD SCH,SANTA MONICA,CA 90407. CALIF STATE UNIV LOS ANGELES,SCH PUBL HLTH,DEPT HLTH SERV,LOS ANGELES,CA 90032. FU AHRQ HHS [1R03HS06920-01] NR 26 TC 50 Z9 50 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0025-7079 J9 MED CARE JI Med. Care PD AUG PY 1995 VL 33 IS 8 BP 842 EP 850 DI 10.1097/00005650-199508000-00008 PG 9 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA RN219 UT WOS:A1995RN21900008 PM 7637405 ER PT J AU STEDMAN, JM NEFF, JA KOPEL, K DONAHOE, CP HAYS, JR AF STEDMAN, JM NEFF, JA KOPEL, K DONAHOE, CP HAYS, JR TI APPLICANT CHARACTERIZATION OF THE MOST DESIRABLE INTERNSHIP TRAINING-PROGRAM SO PROFESSIONAL PSYCHOLOGY-RESEARCH AND PRACTICE LA English DT Article ID PSYCHOLOGY AB One month after Uniform Notification Day, 148 predoctoral internship applicants (49% return rate) were asked to rate their first-, intermediate-, and last-choice sites on 25 variables related to internship program characteristics. Results showed that 8 variables clearly differentiated all three types, and 11 variables differentiated first from last choices. These first-choice characteristics and their implications for program construction and applicant site comparisons are discussed. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. VET ADM MED CTR,HOUSTON,TX 77211. UNIV TEXAS,SCH MED,DEPT PSYCHIAT & BEHAV SCI,HARRIS CTY PSYCHIAT CTR,HOUSTON,TX. RP STEDMAN, JM (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 16 TC 9 Z9 9 U1 0 U2 0 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0735-7028 J9 PROF PSYCHOL-RES PR JI Prof. Psychol.-Res. Pract. PD AUG PY 1995 VL 26 IS 4 BP 396 EP 400 DI 10.1037//0735-7028.26.4.396 PG 5 WC Psychology, Multidisciplinary SC Psychology GA RL505 UT WOS:A1995RL50500008 ER PT J AU MCHORNEY, CA TARLOV, AR AF MCHORNEY, CA TARLOV, AR TI INDIVIDUAL-PATIENT MONITORING IN CLINICAL-PRACTICE - ARE AVAILABLE HEALTH-STATUS SURVEYS ADEQUATE SO QUALITY OF LIFE RESEARCH LA English DT Review DE CLINICAL PRACTICE; HEALTH STATUS ASSESSMENT; INDIVIDUAL PATIENT APPLICATIONS; MEASUREMENT STANDARDS RELIABILITY; SCORE DISTRIBUTIONS; VALIDITY ID QUALITY-OF-LIFE; FUNCTIONAL STATUS QUESTIONNAIRE; PRIMARY CARE PATIENTS; PERCEIVED HEALTH; RHEUMATOID-ARTHRITIS; MEDICAL OUTCOMES; RANDOMIZED TRIAL; SUBJECTIVE HEALTH; STATUS INSTRUMENT; OXYGEN-THERAPY AB Interest has increased in recent years in incorporating health status measures into clinical practice for use at the individual-patient level, We propose six measurement standards for individual-patient applications: (1) practical features, (2) breadth of health measured, (3) depth of health measured, (4) precision for cross-sectional assessment, (5) precision for longitudinal monitoring and (6) validity, We evaluate five health status surveys (Functional Status Questionnaire, Dartmouth COOP Poster Charts, Nottingham Health Profile, Duke Health Profile, and SF-36 Health Survey) that have been proposed for use in clinical practice, We conducted an analytical literature review to evaluate the six measurement standards for individual-patient applications across the five surveys, The most problematic feature of the five surveys was their lack of precision for individual-patient applications, Across all scales, reliability standards for individual assessment and monitoring were not satisfied, and the 95% Cls were very wide, There was little evidence of the validity of the five surveys for screening, diagnosing, or monitoring individual patients, The health status surveys examined in this paper may not be suitable for monitoring the health and treatment status of individual patients, Clinical usefulness of existing measures might be demonstrated as clinical experience is broadened, At this time, however, it seems that new instruments, or adaptation of existing measures and scaling methods, are needed for individual-patient assessment and monitoring. C1 UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,HLTH SERV RES & DEV PROGRAM,MADISON,WI. HARVARD UNIV,SCH PUBL HLTH,NEW ENGLAND MED CTR,INST HLTH,BOSTON,MA 02115. TUFTS UNIV,BOSTON,MA 02111. RP MCHORNEY, CA (reprint author), UNIV WISCONSIN,DEPT PREVENT MED,MADISON,WI 53706, USA. NR 141 TC 818 Z9 822 U1 6 U2 28 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0962-9343 J9 QUAL LIFE RES JI Qual. Life Res. PD AUG PY 1995 VL 4 IS 4 BP 293 EP 307 DI 10.1007/BF01593882 PG 15 WC Health Care Sciences & Services; Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA RP357 UT WOS:A1995RP35700001 PM 7550178 ER PT J AU SHEKELLE, PG MARKOVICH, S LOUIE, R AF SHEKELLE, PG MARKOVICH, S LOUIE, R TI AN EPIDEMIOLOGIC-STUDY OF EPISODES OF BACK PAIN CARE SO SPINE LA English DT Article AB Study Design. This was a prospective community-based, observational design. Objective. To describe the epidemiology and risk/prognostic factors for back pain episodes of care in a population representing the nonelderly in the United States. Summary of Background Data. Previous United States studies of the epidemiology of back pain care have used defined industrial populations or have relied on the patient's recall of symptoms and care. Methods. Claims forms from the RAND Health Insurance Experiment, a randomized controlled trial of the use of health services, were analyzed. Claims forms were selected if one of the patient-designated reasons for the visit was back pain. Visits were grouped into episodes of care. Descriptive statistics were calculated for episodes. Multivariate logistic regression was used to calculate adjusted odds ratios for independent explanatory sociodemographic and health status variables associated with back pain episodes of care. Results. The 3105 adults in the Health Insurance Experiment had a combined 11,171 person-years of exposure. Six-hundred-eighty-six persons (22%) had a total 1020 episodes of back pain care, representing 8825 visits. Seventy-one percent of persons had a single episode during the Health Insurance Experiment, and 40% of these episodes consisted of a single visit. There were 9.1 episodes per 100 person-years. Insurance status, geographic site, white race, lesser education, poorer physical functioning, and greater pain at baseline all were independently associated with having a back pain episode of care. Conclusions. Back pain episodes of care occur commonly in the adult U.S. population, but usually are brief and recur infrequently. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH PUBL HLTH,DEPT HLTH SERV,LOS ANGELES,CA 90024. RP SHEKELLE, PG (reprint author), RAND CORP,GRAD SCH,1700 MAIN ST,POB 2138,SANTA MONICA,CA 90407, USA. FU AHRQ HHS [1R03HS06920-01] NR 0 TC 66 Z9 67 U1 2 U2 3 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0362-2436 J9 SPINE JI SPINE PD AUG 1 PY 1995 VL 20 IS 15 BP 1668 EP 1673 DI 10.1097/00007632-199508000-00004 PG 6 WC Clinical Neurology; Orthopedics SC Neurosciences & Neurology; Orthopedics GA RM966 UT WOS:A1995RM96600004 PM 7482015 ER PT J AU ALSINA, M BOYCE, BF MUNDY, GR ROODMAN, GD AF ALSINA, M BOYCE, BF MUNDY, GR ROODMAN, GD TI AN IN-VIVO MODEL OF HUMAN MULTIPLE-MYELOMA BONE-DISEASE SO STEM CELLS LA English DT Article DE MULTIPLE MYELOMA; BONE DISEASE; OSTEOCLAST; OSTEOBLAST ID CELLS; INTERLEUKIN-1; CYTOKINE; GROWTH AB Osteolytic bone destruction and its complications, such as hypercalcemia, pathologic fractures and nerve compression, are the major source of morbidity in patients with multiple myeloma (MM). The bone destruction in MM is due to increased osteoclast activity, but the mechanisms responsible are not entirely clear. We have utilized a human plasma cell leukemia cell line, ARH-77, that has disseminated growth in mice with severe combined immunodeficiency (SCID) and expresses immunoglobulin G kappa (IgG kappa), as a model for human MM. Fifteen SCID mice were irradiated with 400R and 10 of these were injected with 10(6) ARH-77 cells i.v., 24 h after irradiation, Five mice were used as a control group, Development of bone disease was assessed by blood calcium levels, x-rays and histology, Seven out of seven mice that survived irradiation and received ARH-77 cells developed hind limb paralysis 28-35 days after injection, One hundred percent of these mice developed hypercalcemia (1.35-1.46 mmol/l), a mean of five days after becoming paraplegic, Lytic bone lesions were detected by x-ray in all the hypercalcemic mice examined, No lytic lesions or hypercalcemia developed in the controls, Mice were then sacrificed after developing hypercalcemia. Histologic examination of the ARH-77 mice showed infiltration of myeloma cells in the liver and spleen, Marked infiltration by the tumor was found in vertebrae and long bones, with loss of bony trabeculae and increased osteoclast numbers, Thus, we have developed a model of human MM bone disease which should be very useful to study the pathogenesis of the bone destruction in MM, and to develop novel therapeutic strategies to improve or arrest the bone disease in these patients. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NCI NIH HHS [CA-40035, CA01723]; NIADDK NIH HHS [AM35188] NR 8 TC 9 Z9 9 U1 0 U2 0 PU ALPHAMED PRESS PI DAYTON PA 4100 S KETTERING BLVD, DAYTON, OH 45439-2092 SN 1066-5099 J9 STEM CELLS JI Stem Cells PD AUG PY 1995 VL 13 SU 2 BP 48 EP 50 PG 3 WC Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology SC Cell Biology; Biotechnology & Applied Microbiology; Oncology; Hematology GA RT151 UT WOS:A1995RT15100008 PM 8520511 ER PT J AU BARTKIEWICZ, M HERNANDO, N REDDY, SV ROODMAN, GD BARON, R AF BARTKIEWICZ, M HERNANDO, N REDDY, SV ROODMAN, GD BARON, R TI CHARACTERIZATION OF THE OSTEOCLAST VACUOLAR H+-ATPASE B-SUBUNIT SO GENE LA English DT Article DE PROTON PUMP; V-ATPASE; ION TRANSPORT; BONE RESORPTION; CHICKEN; CDNA CLONING; 1,25-DIHYDROXYVITAMIN D-3 ID CARBONIC ANHYDRASE-II; PROTON PUMP; BONE-RESORPTION; MESSENGER-RNA; BOVINE KIDNEY; EXPRESSION; CELLS; LOCALIZATION; MEMBRANES; ISOFORM AB During bone resorption, osteoclasts acidify the extracellular bone resorbing compartment via a vacuolar H+-ATPase (V-ATPase), which resides in the ruffled-border membrane. In an effort to characterize the composition of the osteoclast V-ATPase catalytic domain, we have isolated a cDNA clone that encodes the V-ATPase B-subunit from a cDNA library constructed from highly purified chicken osteoclasts. Comparison of the predicted amino-acid sequence with the published sequences of isoforms of V-ATPase B-subunits from other sources revealed that the chicken osteoclast B-subunit is brain type and not kidney type. Furthermore, only clones encoding the brain type isoform of subunit B could be generated by PCR from a cDNA library prepared from human osteoclastoma osteoclast-like cells. Northern blot analysis revealed that two B-subunit mRNAs, approx. 1.7 and 3.5 kb in length, are expressed in chicken bone marrow mononuclear cells, brain and kidney, although the relative amounts of these two transcripts were different in each tissue. In brain, the 3.5-kb mRNA was predominantly expressed. In bone marrow cells, the levels of the 1.7-kb mRNA were higher than in other tissues and expression of this message was increased by 1,25-dihydroxyvitamin D-3, suggesting that this mRNA is specifically upregulated during osteoclast differentiation. These results indicate that the B-subunit isoforms present in the catalytic domains of the osteoclast and kidney V-H+-ATPases are different and further suggest that selective expression of isoforms of the B-subunit in these two tissues could provide a structural basis for some of the differences we have reported in the pharmacology and catalytic properties of these two enzymes. C1 YALE UNIV,SCH MED,DEPT ORTHOPED,NEW HAVEN,CT 06520. YALE UNIV,SCH MED,DEPT CELL BIOL,NEW HAVEN,CT 06520. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NIAMS NIH HHS [AR 41339] NR 33 TC 16 Z9 17 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD JUL 28 PY 1995 VL 160 IS 2 BP 157 EP 164 DI 10.1016/0378-1119(95)00228-X PG 8 WC Genetics & Heredity SC Genetics & Heredity GA RP177 UT WOS:A1995RP17700003 PM 7642089 ER PT J AU SIEVER, LJ ROTTER, M LOSONCZY, M GUO, SL MITROPOULOU, V TRESTMAN, R APTER, S ZEMISHLANY, Z SILVERMAN, J HORVATH, TB DAVIDSON, M MOHS, R DAVIS, KL AF SIEVER, LJ ROTTER, M LOSONCZY, M GUO, SL MITROPOULOU, V TRESTMAN, R APTER, S ZEMISHLANY, Z SILVERMAN, J HORVATH, TB DAVIDSON, M MOHS, R DAVIS, KL TI LATERAL VENTRICULAR ENLARGEMENT IN SCHIZOTYPAL PERSONALITY-DISORDER SO PSYCHIATRY RESEARCH LA English DT Article DE COMPUTED TOMOGRAPHY; VENTRICLE-BRAIN RATIO; SCHIZOPHRENIA; PERSONALITY DISORDER ID SCHIZOPHRENIC MOTHERS; BRAIN ABNORMALITIES; HOMOVANILLIC-ACID; INTERVIEW; DEFICITS; SIZE AB Although an increase in the ratio of ventricular space to brain (ventricle-brain ratio, VBR) on computed tomography (CT) has been among the most robust findings in chronic schizophrenia, VBR has not been investigated in a large, well-characterized clinical population of patients with schizotypal personality disorder (SPD), a clinical entity with a phenomenologic, genetic, biological, and treatment response relationship to chronic schizophrenia. Accordingly, CT scans were obtained in 36 male SPD patients, 23 males with other personality disorders, 133 male schizophrenic patients, and 42 male normal volunteers, The mean body of the lateral VER was significantly greater in the SPD patients than in the patients with other personality disorders. The VBR of the SPD patients did not differ significantly from either that of the normal volunteers or the schizophrenic patients but was intermediate between the two groups, There were no correlations with either psychotic-like or deficit-related symptoms of SPD in either the SPD or total personality disorder cohorts, SPD patients, like schizophrenic patients, may have increased VBRs compared with patients with other personality disorders; their VBRs fall between the means of schizophrenic patients and normal control subjects. C1 CUNY MT SINAI SCH MED,DEPT PSYCHIAT,NEW YORK,NY 10029. BRONX PSYCHIAT CTR,NEW YORK,NY 10461. VET ADM MED CTR,FDR,PSYCHIAT SERV 116A,MONTROSE,NY 10548. NEW YORK STATE PSYCHIAT INST & HOSP,NEW YORK,NY 10032. NORTHPORT VET ADM MED CTR,PSYCHIAT SERV,NORTHPORT,NY 11768. RP SIEVER, LJ (reprint author), BRONX VET ADM MED CTR,PSYCHIAT SERV 116A,130 W KINGSBRIDGE RD,BRONX,NY 10468, USA. FU NCRR NIH HHS [RR-0071]; NIA NIH HHS [AG-02219]; NIMH NIH HHS [MH-41131] NR 34 TC 24 Z9 24 U1 0 U2 0 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0165-1781 J9 PSYCHIAT RES JI Psychiatry Res. PD JUL 28 PY 1995 VL 57 IS 2 BP 109 EP 118 DI 10.1016/0165-1781(95)02645-D PG 10 WC Psychiatry SC Psychiatry GA RQ448 UT WOS:A1995RQ44800003 PM 7480378 ER PT J AU YOKOYAMA, H BARAONA, E LIEBER, CS AF YOKOYAMA, H BARAONA, E LIEBER, CS TI COMPARISON OF THE ADH7 GENE STRUCTURE IN CAUCASIAN AND JAPANESE SUBJECTS SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID ALCOHOL-DEHYDROGENASE; STOMACH; ENZYME AB The ADH 7 gene, encoding the sigma-alcohol dehydrogenase isozyme, was cloned from a Caucasian genomic DNA library. Comparison of the nucleotide sequence of its exon 7 with that of an ADH 7 previously cloned from a Japanese subject revealed a substitution of the glycine-287 in the Caucasian sigma isozyme with valine in the Japanese. Since a possible mutation at this site could account for ethnic differences in the gastric activity of this isozyme, the frequency of this change was examined in both races. The exon 7 of the ADH 7 was amplified by PCR from 7 Caucasian and 7 Japanese genomic DNA and applied to restriction fragment length polymorphism analysis, using Ava II to digest the sequence encoding glycine -287 and Mae III to digest that encoding valine-287. Regardless of ethnicity, all PCR amplicons were digested by Ava II and none by Mae III, suggesting that the ethnic difference in the 287 amino acid may represent an uncommon mutation, which does not account for the high frequency of minimal activity of the sigma-alcohol dehydrogenase in the Japanese stomach compared to those of non-Orientals. (C) 1995 Academic Press, Inc. C1 MT SINAI SCH MED,NEW YORK,NY 10468. RP YOKOYAMA, H (reprint author), BRONX VET ADM MED CTR,ALCOHOL RES CTR,NEW YORK,NY 10468, USA. NR 10 TC 7 Z9 7 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JUL 26 PY 1995 VL 212 IS 3 BP 875 EP 878 DI 10.1006/bbrc.1995.2050 PG 4 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA RL363 UT WOS:A1995RL36300021 PM 7626124 ER PT J AU ELKASHEF, AM EGAN, MF FRANK, JA HYDE, TM LEWIS, BK WYATT, RJ AF ELKASHEF, AM EGAN, MF FRANK, JA HYDE, TM LEWIS, BK WYATT, RJ TI MAGNETIC-RESONANCE-IMAGING EVALUATION OF BRAIN IRON LEVELS - RESPONSE SO BIOLOGICAL PSYCHIATRY LA English DT Letter ID HALLERVORDEN-SPATZ DISEASE; TARDIVE-DYSKINESIA; MR RP ELKASHEF, AM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,LOS ANGELES,CA 90073, USA. NR 10 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiat. PD JUL 15 PY 1995 VL 38 IS 2 BP 134 EP 135 PG 2 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA RK731 UT WOS:A1995RK73100016 ER PT J AU MULROW, CD CORNELL, JA KADRI, A FARNETT, L HERRERA, CR AGUILAR, C AF MULROW, CD CORNELL, JA KADRI, A FARNETT, L HERRERA, CR AGUILAR, C TI HYPERTENSION IN THE ELDERLY - CORRECTION AND CLARIFICATION - REPLY SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter C1 UNIV TEXAS,HLTH SCI CTR,HOUSTON,TX. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. RP MULROW, CD (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JUL 5 PY 1995 VL 274 IS 1 BP 25 EP 25 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA RG233 UT WOS:A1995RG23300019 ER PT J AU CHUN, SH SAGER, PT STEVENSON, WG NADEMANEE, K MIDDLEKAUFF, HR SINGH, BN AF CHUN, SH SAGER, PT STEVENSON, WG NADEMANEE, K MIDDLEKAUFF, HR SINGH, BN TI LONG-TERM EFFICACY OF AMIODARONE FOR THE MAINTENANCE OF NORMAL SINUS RHYTHM IN PATIENTS WITH REFRACTORY ATRIAL-FIBRILLATION OR FLUTTER SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID LOW-DOSE AMIODARONE; VENTRICULAR TACHYARRHYTHMIAS; QUINIDINE THERAPY; CONVERSION; CARDIOVERSION; ARRHYTHMIAS; STROKE AB The purpose of this study was to examine the efficacy and safety of amiodarone to maintain sinus rhythm in patients with refractory atrial fibrillation or flutter. One hundred ten patients with atrial fibrillation or flutter, refractory to greater than or equal to 1 class I antiarrhythmic agents (mean +/- SD 2.5 +/- 1.5, median 2), were given low-dose amiodarone (mean maintenance dose 268 +/- 100 mg/day) to determine its efficacy to maintain normal sinus rhythm after chemical or electrical cardioversion. Fifty-three patients had chronic and 57 patients had paroxysmal atrial fibrillation or flutter. Mean age of the study population was 60 +/- 13 years, and the mean follow-up was 36 +/- 38 months (range 31 days to 137 months). Actuarial rates for maintenance of sinus rhythm were 0.87, 0.70, and 0.55 at 1, 3, and 5 years, respectively. Twenty-one patients (19%) with arrhythmia recurrence had an increase in amiodarone dose, and after a mean additional follow-up of 2.5 years, 86% remained in normal sinus rhythm. The only observed predictor of atrial fibrillation or flutter recurrence was paroxysmal arrhythmia (40% recurrence vs 9% in patients with chronic atrial fibrillation or flutter; p<0.001). Actuarial rates for withdrawal because of adverse effects were 0.08, 0.22, and 0.30 at 1, 3, and 5 years, respectively. The most frequent adverse effects necessitating withdrawal were skin discoloration (4.5%), pulmonary fibrosis (3.6%; none fatal), and thyroid toxicity (2.7%). No deaths occurred during the study period. In conclusion, amiodarone has a high degree of efficacy for maintaining normal sinus rhythm in patients with atrial fibrillation or flutter, with a relatively low incidence of adverse effects necessitating withdrawal. C1 W LOS ANGELES VET AFFAIRS MED CTR,DIV CARDIOL,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DIV CARDIOL,LOS ANGELES,CA 90024. NR 22 TC 144 Z9 155 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JUL 1 PY 1995 VL 76 IS 1 BP 47 EP 50 DI 10.1016/S0002-9149(99)80799-1 PG 4 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA RE738 UT WOS:A1995RE73800009 PM 7793402 ER PT J AU LEAF, DA CONNOR, WE BARSTAD, L SEXTON, G AF LEAF, DA CONNOR, WE BARSTAD, L SEXTON, G TI INCORPORATION OF DIETARY N-3 FATTY-ACIDS INTO THE FATTY-ACIDS OF HUMAN ADIPOSE-TISSUE AND PLASMA-LIPID CLASSES SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE ADIPOSE TISSUE; FISH OIL; EICOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID ID CORONARY HEART-DISEASE; EICOSAPENTAENOIC ACID; DOCOSAHEXAENOIC ACID; FISH CONSUMPTION; MEN; OIL; MORTALITY AB The consumption of n-3 fatty acids from seafood has been related to a lower incidence of coronary artery disease. Adipose tissue composition has served as a biological marker of chronic ingestion of many dietary polyunsaturated fatty acids. However, the incorporation of n-3 fatty acids into the fat depots has not been studied in humans. Daily dietary supplementation with greater than or equal to 10 g n-3 fatty acids from fish oil for > 12 mo resulted in significantly greater 20:5n-3, 22:5n-3, and 22:6n-3 concentrations in fatty acids of adipose tissue, and a greater 20:5n-3 fatty acid content in plasma Lipid classes (cholesterol esters, phospholipids, and free fatty acids) of supplemented subjects compared with nonsupplemented control subjects. Combined values for all subjects indicated that fatty acid concentrations of n-3 plasma lipid classes, including 20:5n-3, 22:5n-3, 22:6n-3, and total n-3, significantly correlated with corresponding concentrations of fatty acids in adipose tissue. These findings indicate that the long-term ingestion of large amounts of n-3 fatty acids in humans resulted in their incorporation into the adipose tissue fatty acids. Incorporation of the fatty acids into adipose tissue warrants consideration for use in clinical studies requiring precise documentation of long-term n-3 fatty acid consumption. C1 OREGON HLTH SCI UNIV,DEPT MED,PORTLAND,OR 97201. RP LEAF, DA (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET ADM MED CTR,DEPT MED,DIV GEN INTERNAL MED 69111G,LOS ANGELES,CA 90073, USA. NR 35 TC 84 Z9 85 U1 0 U2 5 PU AMER SOC CLIN NUTRITION INC PI BETHESDA PA 9650 ROCKVILLE PIKE SUBSCRIPTIONS, RM L-2310, BETHESDA, MD 20814-3998 SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUL PY 1995 VL 62 IS 1 BP 68 EP 73 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA RG808 UT WOS:A1995RG80800009 PM 7598068 ER PT J AU CLARK, EC SILVER, SM HOLLICK, GE RINALDI, MG AF CLARK, EC SILVER, SM HOLLICK, GE RINALDI, MG TI CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS COMPLICATED BY AUREOBASIDIUM-PULLULANS PERITONITIS SO AMERICAN JOURNAL OF NEPHROLOGY LA English DT Note DE PERITONITIS; CONTINUOUS AMBULATORY PERITONEAL DIALYSIS; AUREOBASIDIUM; AMPHOTERICIN B; DEMATIACEOUS FUNGUS ID FUNGAL PERITONITIS; INFECTION; PATIENT; CHILDREN AB We describe a case of peritonitis caused by Aureobasidium pullulans in a patient on continuous ambulatory peritoneal dialysis (CAPD). This dematiaceous fungus rarely causes infection in humans and to date has not been reported as an etiology of CAPD-associated peritonitis. The patient was managed successfully with peritoneal catheter removal and a prolonged course of intravenous amphotericin B, allowing resumption of CAPD. In vitro susceptibility testing confirmed sensitivity of this organism to amphotericin B. C1 ROCHESTER GEN HOSP,DEPT PATHOL & LAB MED,ROCHESTER,NY 14621. UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78285. RP CLARK, EC (reprint author), UNIV ROCHESTER,ROCHESTER GEN HOSP,SCH MED & DENT,DEPT MED,NEPHROL UNIT,1425 PORTLAND AVE,ROCHESTER,NY 14621, USA. NR 20 TC 25 Z9 25 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0250-8095 J9 AM J NEPHROL JI Am. J. Nephrol. PD JUL-AUG PY 1995 VL 15 IS 4 BP 353 EP 355 DI 10.1159/000168863 PG 3 WC Urology & Nephrology SC Urology & Nephrology GA RL424 UT WOS:A1995RL42400015 PM 7573197 ER PT J AU SIMON, FR LEFFERT, HL ELLISMAN, M IWAHASHI, M DEERINCK, T FORTUNE, J MORALES, D DAHL, R SUTHERLAND, E AF SIMON, FR LEFFERT, HL ELLISMAN, M IWAHASHI, M DEERINCK, T FORTUNE, J MORALES, D DAHL, R SUTHERLAND, E TI HEPATIC NA+-K+-ATPASE ENZYME-ACTIVITY CORRELATES WITH POLARIZED BETA-SUBUNIT EXPRESSION SO AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY LA English DT Article DE IMMUNOFLUORESCENCE; IMMUNOGOLD LABELING; SINUSOIDAL; BILE CANALICULAR; BASOLATERAL DOMAIN; SODIUM-POTASSIUM ADENOSINE-TRIPHOSPHATASE ID NA+,K+-ATPASE ALPHA-SUBUNIT; TISSUE-SPECIFIC EXPRESSION; MEMBRANE LIPID FLUIDITY; RAT-BRAIN; CATALYTIC SUBUNIT; IMMUNOELECTRON MICROSCOPY; ADENOSINE-TRIPHOSPHATASE; DIFFERENTIAL EXPRESSION; PLASMA-MEMBRANES; NERVOUS-SYSTEM AB We have examined underlying causes for observations made in hepatocytes in which catalytic subunits of Na+-K+-ATPase are found both in bile canalicular (apical) and sinusoidal (basolateral) membrane domains, whereas functional activity is associated preferentially with sinusoidal membrane sites. In a series of parallel studies, we determined by both light and electron microscopy that Na+-K+-ATPase alpha-subunits were localized to both membrane domains of hepatocytes. With the use of purified liver plasma membrane subfractions, ouabain inhibition curves demonstrated similar inhibition constants (inhibition constant 10(-5) M), and immunoblots using alpha(1)-, alpha(2)-, alpha(3)-polyclonal and monoclonal antibodies demonstrated antigenic sites predominantly for alpha(1) in both membrane fractions. Also, Northern blot hybridization analysis revealed only the alpha(1)-isoform in hepatocytes. In contrast to the bipolar distribution of the alpha(1)-subunit, the beta-subunit was identified only at the sinusoidal surface using fluorescence labeling with a monoclonal antibody. The beta(1)-isoform was demonstrated by Northern blot analysis and was present predominantly at the sinusoidal domain by immunoblotting with polyclonal antibodies. In addition to the bipolar distribution of oil, immunoblotting of liver plasma membrane subfractions demonstrated a symmetrical distribution of fodrin, ankyrin, actin, and E-cadherin at both domains. These results suggest that functionally competent alpha/beta-complexes form at the sinusoidal domain, whereas only alpha(1)-subunits are present at the apical pole. C1 DENVER VET AFFAIRS HOSP, DEPT MED, DENVER, CO 80262 USA. UNIV CALIF SAN DIEGO, DEPT PHARMACOL, LA JOLLA, CA 92093 USA. UNIV CALIF SAN DIEGO, CTR MOLEC GENET & NEUROBIOL, LA JOLLA, CA 92093 USA. RP SIMON, FR (reprint author), UNIV COLORADO, SCH MED, DEPT MED B145, HEPATOBILIARY RES CTR, 4200 E 9TH AVE, DENVER, CO 80262 USA. FU NIDDK NIH HHS [DK-15851, DK-28215, DK-34914] NR 51 TC 18 Z9 18 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6143 J9 AM J PHYSIOL-CELL PH JI Am. J. Physiol.-Cell Physiol. PD JUL PY 1995 VL 269 IS 1 BP C69 EP C84 PG 16 WC Cell Biology; Physiology SC Cell Biology; Physiology GA RK171 UT WOS:A1995RK17100011 PM 7631761 ER PT J AU LEE, FT DELONE, DR BEAN, DW BROGHAMMER, BG MACK, E PERLMAN, SB WEISS, JW AF LEE, FT DELONE, DR BEAN, DW BROGHAMMER, BG MACK, E PERLMAN, SB WEISS, JW TI ACUTE CHOLECYSTITIS IN AN ANIMAL-MODEL - FINDINGS ON COLOR DOPPLER SONOGRAPHY SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID GALLBLADDER; MANAGEMENT; OPERATION; DIAGNOSIS AB OBJECTIVE. The purpose of our study was to evaluate the color Doppler findings of acute cholecystitis in a controlled canine model. MATERIALS AND METHODS. Fourteen animals had a laparotomy: cystic duet ligation was done in eight, and incision with closure was performed in six control subjects. Animals were scanned in a blinded fashion preoperatively, immediately postoperatively, and on postoperative days 1-5. On postoperative day 5, a hepatobiliary scan was done with 2 mCi (74 MBq) Tc-99m-mebrofenin. Blinded histopathology was performed and correlated with imaging. RESULTS. Flow was seen in the wall of each gallbladder at some point during the postoperative course, demonstrating vascular patency Hepatobiliary scintigraphy confirmed cystic duct status in 12 cases; two animals died before radionuclide imaging was complete. Color Doppler signal decreased in the gallbladder wall in ligated dogs from postoperative day 1 to postoperative day 3 (p = .03 versus controls at postoperative day 2) and increasingly returned by postoperative day 5. Hyperemia was seen in only two cases (both with severe necrotizing cholecystitis) and only at postoperative day 5. Although not statistically significant, a weak trend of increasing flow with more severe pathologic grades of cholecystitis was observed (p = .20). CONCLUSIONS. in this animal model, loss of vascular signal (not hyperemia) at postoperative day 2 was the finding to diagnose early acute cholecystitis, although lack of flow can also be seen in some normal subjects. Flow tended to return by postoperative day 5, and it increased in some of the more severe cases of cholecystitis. Hyperemia was a somewhat useful sign of acute necrotizing cholecystitis. C1 UNIV WISCONSIN HOSP & CLIN,DEPT SURG,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT PATHOL,MADISON,WI 53705. RP LEE, FT (reprint author), UNIV WISCONSIN HOSP & CLIN,DEPT RADIOL,600 HIGHLAND AVE,MADISON,WI 53792, USA. OI DeLone, David/0000-0002-4211-8790 NR 23 TC 4 Z9 4 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JUL PY 1995 VL 165 IS 1 BP 85 EP 90 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA RE240 UT WOS:A1995RE24000023 PM 7785640 ER PT J AU HUNT, DK BADGETT, RG WOODLING, AE PUGH, JA AF HUNT, DK BADGETT, RG WOODLING, AE PUGH, JA TI MEDICAL-STUDENT CAREER CHOICE - DO PHYSICAL DIAGNOSIS PRECEPTORS INFLUENCE DECISIONS SO AMERICAN JOURNAL OF THE MEDICAL SCIENCES LA English DT Article DE MEDICAL EDUCATION; CAREER CHOICE; MEDICAL STUDENTS; PHYSICAL EXAMINATION ID INTERNAL-MEDICINE; SPECIALTY; SCHOOL; ATTITUDES AB The authors attempted to measure the influence of a physical diagnosis course and its preceptors on the career decisions of second-year medical students. They designed pre- and post-course questionnaires for 204 second-year medical students in a University of Texas Health Science Center at San Antonio Advanced Physical Diagnosis course. They found that 48% of students changed their career choice during the study period, 75% believed their preceptor was a very good role model, and 39% thought their preceptor influenced their career choice. Students who believed their preceptor was a good role model were 31 times more likely to consider their preceptors' career (confidence interval [CI] 95, 4.1-236). In results from students precepted by primary care physicians, there was a nonsignificant trend toward choosing a primary care career (Odds Ratio [OR]) 1.6 [CI95, 0.7-3.3]). Factors associated with a final career choice of primary care were a primary care career choice at baseline (OR 8.5 [CI95, 3.8-19.0]) and a belief that physical diagnosis skills would be important to a future career (OR 4.7 [CI95, 1.1-20.0]). By multivariable analysis, only a primary care career choice at baseline remained significant (OR 8.7 [CI95, 3.5-21.3]). The authors concluded that good role models can influence students to consider alternative career choices, but this effect is still overshadowed by a student's baseline career choice. This reinforces the importance of selecting medical school applicants already inclined toward a primary care career. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. MEXICAN AMER MED TREATMENT EFFECTIVENESS CTR,SAN ANTONIO,TX. RP HUNT, DK (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GEN INTERNAL MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. OI Pugh, Jacqueline/0000-0003-4933-141X FU AHRQ HHS [UO1-HS07397]; NIDDK NIH HHS [R01-DK38392] NR 20 TC 12 Z9 12 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0002-9629 J9 AM J MED SCI JI Am. J. Med. Sci. PD JUL PY 1995 VL 310 IS 1 BP 19 EP 23 DI 10.1097/00000441-199507000-00007 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA RG997 UT WOS:A1995RG99700006 PM 7604834 ER PT J AU LANGBEIN, WE MAKI, KC AF LANGBEIN, WE MAKI, KC TI PREDICTING OXYGEN-UPTAKE DURING COUNTERCLOCKWISE ARM CRANK ERGOMETRY IN MEN WITH LOWER-LIMB DISABILITIES SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Article ID PHYSIOLOGICAL-RESPONSES; EXERCISE AB Objective: This study was conducted to develop a new equation for predicting oxygen uptake (VO2) during counterclockwise arm crank ergometry (ACE) in men with lower limb disabilities, cross-validate the new equation in a similar group of men, and compare the predictive accuracy of the new equation to previously published equations for clockwise ACE. Patients: The metabolic responses of 55 men, 17 to 69 years of age, with spinal cord injuries (n = 50) or lower limb fractures (n = 5) were recorded during maximal ACE-graded exercise tests. Participants were volunteers from area hospital rehabilitation centers, and wheelchair sport teams. Design: Subjects were partitioned by level of injury and randomly assigned to a prediction (PRE) or validation (VAL) group. Results: No differences were found between the PRE and VAL groups for age, anthropometric or peak exercise variables. Using stepwise regression, a prediction equation (EXP) was derived from the PRE group data. The resulting model: VO2 (mL/min) = 127.06 + 7.201 (Watts) + 4.502 (weight in kg) + 0.033 (Watts(2)) explained 89.8% of the variance in the PRE group VO2, standard error of estimate (SEE) = 151.9mL/min. The equation performed similarly in the VAL group (SEE = 144.0mL/min). Conclusions: In this sample, the EXP equation had less prediction error than equations derived for clockwise ACE. Accuracy was not substantially influenced by level of injury. Comparison of the data to that published previously for clockwise ACE by men with paraplegia suggests greater metabolic economy for counterclockwise as compared with clockwise ACE. (C) 1995 by the American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation RP LANGBEIN, WE (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,CTR REHABIL RES & DEV,POB 20,HINES,IL 60141, USA. NR 16 TC 9 Z9 9 U1 1 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD JUL PY 1995 VL 76 IS 7 BP 642 EP 646 DI 10.1016/S0003-9993(95)80633-4 PG 5 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA RH676 UT WOS:A1995RH67600007 PM 7605183 ER PT J AU MOHAGHEGHPOUR, N ABEL, K LAPAGLIA, N EMANUELE, NV AZAD, N AF MOHAGHEGHPOUR, N ABEL, K LAPAGLIA, N EMANUELE, NV AZAD, N TI SIGNAL REQUIREMENTS FOR PRODUCTION OF LUTEINIZING-HORMONE RELEASING-HORMONE BY HUMAN T-CELLS SO CELLULAR IMMUNOLOGY LA English DT Article ID PROTEIN KINASE-C; CYTOPLASMIC FREE CALCIUM; TYROSINE PHOSPHORYLATION; SURFACE MOLECULES; ANTIGEN RECEPTOR; PHOSPHOLIPASE C-GAMMA-1; INTERLEUKIN-2 RECEPTOR; LHRH RECEPTORS; PHORBOL ESTERS; MESSENGER-RNA AB We have previously demonstrated that both human CD4(+) and CD8(+) T lymphocytes produce enhanced levels of luteinizing hormone-releasing hormone (LHRH) mRNA and peptide upon stimulation with monoclonal antibody directed at the CD3 component of the T cell receptor for antigen (TCR) or mitogenic lectin, In the current study, we define the signaling pathways that control TCR-mediated LHRH production by using agents known to affect distinct signals, and compare the messenger systems required for LHRH response to other T-cell-associated activation responses, such as expression of CD69 and interleukin-2 receptor (IL-2R) molecules and production of interleukin-2 (IL-2), Results indicate that the activation of protein kinase C (PKC) is essential for LHRH production by previously nonstimulated T cells, not increased concentration of cytosolic-free calcium ([Ca2+](i)), Phorbol ester (PMA), a direct activator of PKC, provoked LHRH production and cell surface expression of CD69 and IL-BR molecules by T cells, but not IL-2 synthesis, The synthesis of IL-2 by T cells required costimulation with PMA and ionomycin, a Ca2+ ionophore, Consistent with these observations, H7, an inhibitor of PKC, prevented T cells from producing LHRH upon activation with mitogen, However, LHRH production was not suppressed by HA1004, which inhibits all cyclic nucleotide-dependent protein kinases except for PKC, Genistein, a selective inhibitor of protein tyrosine kinase, blocked PMA-induced increase in LHRH production, but not CD69 and IL-2R expression, suggesting that protein tyrosine phosphorylation events distal from PKC activation may play a role in regulating LHRH response, (C) 1995 Academic Press, Inc. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,RES SERV,HINES,IL 60141. US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,MED SERV,HINES,IL 60141. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,PROGRAM MOLEC BIOL,MAYWOOD,IL 60153. RP MOHAGHEGHPOUR, N (reprint author), CALIF PACIFIC MED CTR,RES INST,KUZELL INST ARTHRITIS & INFECT DIS,2200 WEBSTER ST,SUITE 305,SAN FRANCISCO,CA 94115, USA. NR 60 TC 7 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0008-8749 J9 CELL IMMUNOL JI Cell. Immunol. PD JUL PY 1995 VL 163 IS 2 BP 280 EP 288 DI 10.1006/cimm.1995.1127 PG 9 WC Cell Biology; Immunology SC Cell Biology; Immunology GA RH853 UT WOS:A1995RH85300015 PM 7606799 ER PT J AU MARGOLIS, ML AF MARGOLIS, ML TI DOES A POSITIVE BRAIN CT REFLECT BRAIN METASTASES SO CHEST LA English DT Letter RP MARGOLIS, ML (reprint author), PHILADELPHIA VA MED CTR,PHILADELPHIA,PA, USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD JUL PY 1995 VL 108 IS 1 BP 295 EP 296 DI 10.1378/chest.108.1.295-b PG 2 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA RJ222 UT WOS:A1995RJ22200071 PM 7606985 ER PT J AU GUARRO, J SOLER, L RINALDI, MG AF GUARRO, J SOLER, L RINALDI, MG TI PATHOGENICITY AND ANTIFUNGAL SUSCEPTIBILITY OF CHAETOMIUM SPECIES SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Note ID GLOBOSUM; ONYCHOMYCOSIS; KUNZE AB Several reports have been published implicating Chaetomium spp, as opportunistic pathogens. A critical review of these cases was made, and the majority of the responsible strains were studied. Chaetomium globosum was the most common species, being isolated in at least nine clinical cases of infection. Some of these clinical isolates and others from environmental sources were tested against six antifungal agents (5-fluorocytosine, fluconazole, amphotericin B, itraconazole, ketoconazole and miconazole). The 23 strains tested were totally resistant to the first two drugs, and none of the other antifungal agents demonstrated fungicidal activity. There were no significant differences between the susceptibility of the clinical strains and the other strains. C1 UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,CTR HLTH SCI,DEPT PATHOL,LAB SERV 113,SAN ANTONIO,TX 78284. RP GUARRO, J (reprint author), UNIV ROVIRA & VIRGILI,FAC MED,UNITAT MICROBIOL,C ST LLORENC 21,E-43201 REUS,SPAIN. OI Guarro, Josep/0000-0002-7839-7568 NR 16 TC 31 Z9 31 U1 1 U2 3 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD JUL PY 1995 VL 14 IS 7 BP 613 EP 618 DI 10.1007/BF01690737 PG 6 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA RQ617 UT WOS:A1995RQ61700011 PM 7588850 ER PT J AU CRAIG, WA AF CRAIG, WA TI ANTIBIOTIC SELECTION FACTORS AND DESCRIPTION OF A HOSPITAL-BASED OUTPATIENT ANTIBIOTIC-THERAPY PROGRAM IN THE USA SO EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES LA English DT Article ID PEAK CONCENTRATION; INFECTION MODELS; PENICILLIN-G; EFFICACY; INVITRO; PHARMACODYNAMICS; SUSCEPTIBILITY; ACCUMULATION; RESISTANCE; NETILMICIN AB A variety of pharmacodynamic, pharmacokinetic and drug stability factors can influence the choice of drug, the dosing regimen and the method of drug administration for outpatient parenteral antibiotic therapy (OPAT). Beta-lactam antibiotics exhibit little if any concentration-dependent killing and produce short-term or no persistent effects with most bacterial pathogens. Optimal dosing regimens for these agents should provide serum levels that continually exceed the minimal inhibitory concentration (MIC) of the pathogen. Beta-lactam agents with long half-lives (greater than 2 hours) can provide these levels with intermittent dosing once or twice daily. Beta-lactam agents with shorter half-lives can be administered by programmable pumps or by continuous infusion providing the drug is sufficiently stable to degradation in solution. Imipenem and ampicillin are examples of drugs with short half-lives that are unstable in solution and must be dosed intermittently. Intramuscular administration slows absorption and can also prolong the length of time during which serum levels exceed the MIC of infecting bacteria. Aminoglycosides and fluoroquinolones, on the other hand, exhibit concentration-dependent killing and produce prolonged persistent effects. Optimal dosage regimens of these drugs should maximize serum levels. Once-daily dosing regimens for the aminoglycosides meet this goal and also appear to reduce drug-induced nephrotoxicity. Application of these principles to drug selection and administration in a hospital-based OPAT program has provided efficacious therapy and a low incidence of adverse reactions in an elderly population distributed over a wide geographic area. C1 UNIV WISCONSIN,MADISON,WI 53705. RP CRAIG, WA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 27 TC 36 Z9 37 U1 0 U2 0 PU FRIEDR VIEWEG SOHN VERLAG GMBH PI WIESBADEN 1 PA PO BOX 5829, W-6200 WIESBADEN 1, GERMANY SN 0934-9723 J9 EUR J CLIN MICROBIOL JI Eur. J. Clin. Microbiol. Infect. Dis. PD JUL PY 1995 VL 14 IS 7 BP 636 EP 642 DI 10.1007/BF01690745 PG 7 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA RQ617 UT WOS:A1995RQ61700019 PM 7588857 ER PT J AU OBERLEY, TD SCHULTZ, JL LI, N OBERLEY, LW AF OBERLEY, TD SCHULTZ, JL LI, N OBERLEY, LW TI ANTIOXIDANT ENZYME LEVELS AS A FUNCTION OF GROWTH-STATE IN CELL-CULTURE SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE MANGANESE SUPEROXIDE DISMUTASE; CELL CULTURE; DENSITY INHIBITION OF GROWTH; CELL CYCLE; FREE RADICALS ID MANGANESE SUPEROXIDE-DISMUTASE; MOUSE EPIDERMAL-CELLS; PROTEIN KINASE-C; HYDROGEN-PEROXIDE; CU,ZN-SUPEROXIDE DISMUTASE; ORNITHINE DECARBOXYLASE; GLUTATHIONE-PEROXIDASE; INTRACELLULAR PH; OXIDATIVE STRESS; EPITHELIAL-CELLS AB Manganese superoxide dismutase (MnSOD) levels were monitored as a function of time in culture to determine whether these levels were altered at logarithmic growth versus when the cells exhibited density limitation of growth. For comparison, activities of the antioxidant enzymes copper, zinc superoxide dismutase (CuZnSOD), catalase, and glutathione peroxidase were also evaluated. Four cell lines were studied, two of which exhibited density limitation of growth and two of which did not. Each cell line showed a unique antioxidant enzyme profile. The two cell lines that showed density limitation of growth also demonstrated induction of MnSOD at the time when the cells stopped proliferating in culture, whereas the other two cell lines did not show induction of MnSOD. There was no strict correlation between density limitation of growth and activities of the other antioxidant enzymes. To determine whether SOD varied with various phases of the cell cycle, NIH/3T3 cells were synchronized using serum starvation, and then SOD activities were measured during quiescence (G(0)) and the phase of DNA synthesis (S-phase). MnSOD was decreased during S-phase compared with G(0), whereas CuZnSOD was increased during S-phase compared with G(0), demonstrating alteration of SOD activities with varying phases of the cell cycle. This study suggests the possibility that increased MnSOD may correlate with decreased cell proliferation and suggests significant alterations in SOD activities during the cell cycle. C1 UNIV WISCONSIN,SCH MED,DEPT PATHOL,MADISON,WI 53706. UNIV IOWA,COLL MED,RADIAT RES LAB,IOWA CITY,IA. RP OBERLEY, TD (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL & LAB MED SERV,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU NCI NIH HHS [CA 41267] NR 52 TC 76 Z9 77 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD JUL PY 1995 VL 19 IS 1 BP 53 EP 65 DI 10.1016/0891-5849(95)00012-M PG 13 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA QZ423 UT WOS:A1995QZ42300007 PM 7635359 ER PT J AU MERTZ, H NALIBOFF, B MUNAKATA, J NIAZI, N MAYER, EA AF MERTZ, H NALIBOFF, B MUNAKATA, J NIAZI, N MAYER, EA TI ALTERED RECTAL PERCEPTION IS A BIOLOGICAL MARKER OF PATIENTS WITH IRRITABLE-BOWEL-SYNDROME SO GASTROENTEROLOGY LA English DT Article ID PAIN; NEURONS; NERVES; DISTENSION; MECHANISMS AB Background and Aims: Lowered visceral perception thresholds have been suggested as a biological marker of irritable bowel syndrome (IBS). The current study sought to determine the prevalence of altered rectal visceral perception in patients with IBS and the correlation of altered perception thresholds with subjective symptoms. Methods: Anorectal manometry and rectal perception thresholds to balloon distention were determined in 100 patients with IBS and 15 control subjects. Gastrointestinal and psychological symptoms were assessed by questionnaire. Perception thresholds and symptoms were reassessed after 3 months in 15 patients with IBS. Results: Ninety-four percent of patients showed altered rectal perception in the form of lowered thresholds for aversive sensations (discomfort), increased intensity of sensations, or altered viscerosomatic referral. Hypersensitivity was found only for aversive sensations in response to rapid phasic distention; stool thresholds and thresholds in response to slow ramp distention were normal. Cluster analysis by physiological parameters identified three IBS subgroups with predominant patterns of symptoms. Longitudinal evaluation indicated a correlation between changes in perception thresholds and symptom severity. Conclusions: Because altered rectal perception is present in almost all patients with IBS and perception thresholds correlate with temporal changes in retrospective symptom severity, altered rectal perception represents a reliable biological marker of IBS. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,VET ADM UCLA CURE GASTROENTER BIOL CTR,DEPT MED,NEUROENTER BIOL GRP,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA. VET ADM MED CTR,SEPULVEDA,CA 91343. FU NIDDK NIH HHS [DK40919] NR 42 TC 705 Z9 728 U1 2 U2 11 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD JUL PY 1995 VL 109 IS 1 BP 40 EP 52 DI 10.1016/0016-5085(95)90267-8 PG 13 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RG191 UT WOS:A1995RG19100005 PM 7797041 ER PT J AU JACOBY, RF MARSHALL, DJ KAILAS, S SCHLACK, S HARMS, B LOVE, R AF JACOBY, RF MARSHALL, DJ KAILAS, S SCHLACK, S HARMS, B LOVE, R TI GENETIC INSTABILITY ASSOCIATED WITH ADENOMA TO CARCINOMA PROGRESSION IN HEREDITARY NONPOLYPOSIS COLON-CANCER SO GASTROENTEROLOGY LA English DT Article ID FAMILIAL COLORECTAL-CANCER; MUTATIONS; LOCUS; DNA; MAP; EVOLUTION; HOMOLOG; GENOME AB Background & Aims: Genetic instability related to defective DNA mismatch repair genes may be involved in the pathogenesis of carcinoma in hereditary nonpolyposis colon cancer (HNPCC). However, nonneoplastic tissues from patients inheriting defects in human MSH2 or human MLH1 do not show significant genetic instability. The aim of this study was to determine whether acquisition of genetic instability at the adenoma stage promotes malignant transformation by studying adenoma-carcinoma progression in HNPCC. Methods: Dinucleotide repeat loci were analyzed by polymerase chain reaction from microdissected adenoma and/or carcinoma stages from formalin-fixed paraffin-embedded HNPCC tumors. Results: Although genetic instability was observed at some loci in almost all cases, the proportion of microsatellite loci altered was significantly less (P < 0.01) in completely benign adenomas (24%) than in benign areas of adenomas with malignancy (54%). Molecular fingerprints indicated intratumor heterogeneity, with evolution of related subclones of neoplastic cells. However, in all cases of tumor progression, at least one subclone from the adenoma stage was closely related to the carcinoma. Conclusions: Some genetic instability develops at the benign adenoma stage in most HNPCC tumors. Adenomas with a greater rate of genetic instability are more likely to progress to carcinoma. Topographic genotyping data provides evidence supporting the hypothesis of adenoma-carcinoma progression in HNPCC. C1 UNIV WISCONSIN,DIV GASTROENTEROL,MADISON,WI. UNIV WISCONSIN,CTR COMPREHENS CANC,MADISON,WI. UNIV WISCONSIN,DEPT SURG,MADISON,WI. UNIV WISCONSIN,DEPT HUMAN ONCOL,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,NJ. FU NCI NIH HHS [U01 CA59352, P30 CA14520] NR 37 TC 92 Z9 93 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD JUL PY 1995 VL 109 IS 1 BP 73 EP 82 DI 10.1016/0016-5085(95)90270-8 PG 10 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RG191 UT WOS:A1995RG19100008 PM 7797042 ER PT J AU SINGARAM, C SWEET, MA GAUMNITZ, EA CAMERON, AJ CAMILLERI, M AF SINGARAM, C SWEET, MA GAUMNITZ, EA CAMERON, AJ CAMILLERI, M TI PEPTIDERGIC AND NITRINERGIC DENERVATION IN CONGENITAL ESOPHAGEAL STENOSIS SO GASTROENTEROLOGY LA English DT Note ID NITRIC-OXIDE SYNTHASE; HIRSCHSPRUNGS-DISEASE AB Congenital esophageal stenosis (CES) is a rare disorder with narrowed esophageal lumen that presents as dysphagia from childhood and that is often associated with tracheobronchial remnants or webs. The pathogenesis of CES is unknown. The aim of this study was to examine the histological and immunohistochemical features of CES. Esophagi from 2 young adults with CES and 3 controls with no motility disorders underwent routine H&E staining, trichrome staining for collagen, and detailed immunocytochemical studies for general neuronal markers (protein gene product 9.5, neuron-specific enolase, and S-100) and neurotransmitters (vasoactive intestinal polypeptide, substance P, and galanin) and nitric oxide synthase by beta-nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase and a specific NO synthase antibody. Quantitative experiments compared the numbers of myenteric neurons and amounts of fibers at the circular muscle. CES esophagi showed infiltration of neutrophils in the myenteric plane, without any increase in collagen. NADPH-diaphorase histochemistry showed a significant reduction of myenteric nitrinergic neurons (7 +/- 3.4 vs. 2.7 +/- 1.8 neurons per high-power field) and fibers at the circular muscle. Other peptidergic neurons studied were not significantly reduced in CES. The specific total lack of NO inhibitory innervation may be an important mechanism in the pathogenesis of stenosis and aperistalsis of the esophagus in this disorder. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. MAYO CLIN,DEPT MED,DIV GASTROENTEROL,ROCHESTER,MN. RP SINGARAM, C (reprint author), UNIV WISCONSIN,CTR CLIN SCI,DEPT MED,DIV GASTROENTEROL,ROOM H6-516,600 HIGHLAND AVE,MADISON,WI 53792, USA. NR 19 TC 27 Z9 29 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD JUL PY 1995 VL 109 IS 1 BP 275 EP 281 DI 10.1016/0016-5085(95)90294-5 PG 7 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA RG191 UT WOS:A1995RG19100032 PM 7541000 ER PT J AU KOPELOWICZ, A LIBERMAN, RP AF KOPELOWICZ, A LIBERMAN, RP TI BIOBEHAVIORAL TREATMENT AND REHABILITATION OF SCHIZOPHRENIA SO HARVARD REVIEW OF PSYCHIATRY LA English DT Review ID SEVERELY MENTALLY-ILL; SOCIAL SKILLS; CASE-MANAGEMENT; LONG-TERM; EXPRESSED EMOTION; CONTROLLED TRIAL; FOLLOW-UP; RELAPSE; FAMILY; VULNERABILITY AB The psychopathology and associated disabilities experienced by persons with schizophrenia have only partially responded to conventional pharmacological and psychosocial treatment approaches. Biobehavioral treatment and rehabilitation employs behavioral assessment, social learning principles, skills training, and a focus on the recovery process to amplify the effects of pharmacotherapy. Utilizing the Medline database, we review a selection of English-language studies published from 1970 to 1994 that support the effectiveness of each of the components of biobehavioral therapy such as case management, psychopharmacology with behavioral assessment, psychoeducation, family involvement, and social skills training. An integrated biobehavioral therapy directed toward early detection and treatment of schizophrenic symptoms, collaboration between consumers and caregivers in managing treatment, family and social skills training, and teaching coping skills and self-help techniques has been documented to improve the course and outcome of schizophrenia, as measured by symptom recurrence, social functioning, and quality of life. A case vignette is presented to illustrate the successful integration of biobehavioral therapies into a treatment system that focuses on consumers' attempts to become increasingly responsible for recovering from illness. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,CAMARILLO NEUROPSYCHIAT RES CTR,LOS ANGELES,CA. RP KOPELOWICZ, A (reprint author), SAN FERNANDO MENTAL HLTH CTR,15535 SAN FERNANDO MISSION BLVD,MISSION HILLS,CA 91345, USA. OI kopelowicz, alex/0000-0002-1728-4105 NR 81 TC 27 Z9 27 U1 2 U2 3 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1067-3229 J9 HARVARD REV PSYCHIAT JI Harv. Rev. Psychiatr. PD JUL-AUG PY 1995 VL 3 IS 2 BP 55 EP 64 DI 10.3109/10673229509017168 PG 10 WC Psychiatry SC Psychiatry GA RL894 UT WOS:A1995RL89400001 PM 9384930 ER PT J AU HARTMAN, N VORON, SC HERSHMAN, JM AF HARTMAN, N VORON, SC HERSHMAN, JM TI RESOLUTION OF MIGRAINE FOLLOWING BROMOCRIPTINE TREATMENT OF A PROLACTINOMA (PITUITARY MICROADENOMA) SO HEADACHE LA English DT Note DE MIGRAINE; BROMOCRIPTINE; PROLACTIN; HYPERPROLACTINEMIA; PROLACTINOMA; PITUITARY NEOPLASM ID HYPERPROLACTINEMIA AB A 39-year-old male physician with a 27-year history of chronic severe migraine had a prolactin-secreting pituitary microadenoma diagnosed as an incidental finding following an automobile accident. Treatment of the prolactinoma with bromocriptine provided complete and lasting resolution of the migraine as well, suggesting a possible etiologic relationship between these two prevalent conditions, and the possibility of treating st least some cases of migriane with bromocriptine. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA. RP HARTMAN, N (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 6 TC 9 Z9 9 U1 0 U2 0 PU AMER ASSOC STUDY HEADACHE PI WOODBURY PA 875 KINGS HIGHWAY, STE 200, WOODBURY, NJ 08096 SN 0017-8748 J9 HEADACHE JI Headache PD JUL-AUG PY 1995 VL 35 IS 7 BP 430 EP 431 DI 10.1111/j.1526-4610.1995.hed3507430.x PG 2 WC Clinical Neurology SC Neurosciences & Neurology GA RM335 UT WOS:A1995RM33500011 PM 7672963 ER PT J AU PAHLAVANI, MA HARRIS, MD AF PAHLAVANI, MA HARRIS, MD TI EFFECT OF DEHYDROEPIANDROSTERONE ON MITOGEN-INDUCED LYMPHOCYTE-PROLIFERATION AND CYTOKINE PRODUCTION IN YOUNG AND OLD F344 RATS SO IMMUNOLOGY LETTERS LA English DT Article DE DHEA; IL-2; IFN-GAMMA; AGING; (RAT) ID LYMPHOKINE PRODUCTION INVIVO; AGE-RELATED DECLINE; CD4+ T-CELLS; INTERLEUKIN-2 SYNTHESIS; INTERFERON-GAMMA; IMMUNE FUNCTION; IL-2 PRODUCTION; MICE; EXPRESSION; INHIBITION AB The steroid hormone intermediate, dehydroepiandrosterone (DHEA), has been proposed as a therapeutic agent for the treatment of immunosenescence in mouse model. In the present study, the in vitro effect of DHEA on mitogen-induced lymphocyte proliferation and cytokine production was evaluated in a rat model. Spleen lymphocytes were isolated from young (4-6 months) and old (24-26 months) F344 rats and were incubated with DHEA for 30 min. The induction of lymphocyte proliferation, interleukin-2 (IL-2), and interferon-gamma (IFN-gamma) production by concanavalin A (Con A) was measured in a culture medium supplemented with either fetal calf serum (FCS) or with serum-free medium (Nutridoma-SR, N-SR). The induction of lymphocyte proliferation and IL-2 production by Con A decreased significantly with age, whereas induction of IFN-gamma increased with age. Treatment of lymphocytes with DHEA did not significantly alter Con A-induced proliferation or the production of IL-2 or IFN-gamma by spleen lymphocytes isolated from either young or old rats. These data indicate that in vitro DHEA treatment appears to have no immunomodulatory effect on the age-related changes in mitogen-induced proliferation or cytokine production in rat lymphocytes. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GERIATR & GERONTOL,SAN ANTONIO,TX 78284. RP PAHLAVANI, MA (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. FU NIA NIH HHS [AG-01548] NR 49 TC 15 Z9 18 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2478 J9 IMMUNOL LETT JI Immunol. Lett. PD JUL-AUG PY 1995 VL 47 IS 1-2 BP 9 EP 14 DI 10.1016/0165-2478(95)00057-C PG 6 WC Immunology SC Immunology GA RU972 UT WOS:A1995RU97200002 PM 8537107 ER PT J AU MORGAN, BJ CRABTREE, DC PALTA, M SKATRUD, JB AF MORGAN, BJ CRABTREE, DC PALTA, M SKATRUD, JB TI COMBINED HYPOXIA AND HYPERCAPNIA EVOKES LONG-LASTING SYMPATHETIC ACTIVATION IN HUMANS SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE SYMPATHETIC NERVOUS SYSTEM ID BODY NEGATIVE-PRESSURE; BLOOD-PRESSURE; SLEEP-APNEA; TERM POTENTIATION; EPISODIC HYPOXIA; CARDIAC-OUTPUT; RESPONSES; NOREPINEPHRINE; CARDIOGRAPHY; VENTILATION AB We studied ventilatory and neurocirculatory responses to combined hypoxia (arterial O-2 saturation 80%) and hypercapnia (end-tidal CO2 + 5 Torr) in awake humans. This asphyxic stimulus produced a substantial increase in minute ventilation (6.9 +/- 0.4 to 20.0 +/- 1.5 l/min) that promptly subsided on return to room air breathing. During asphyxia, muscle sympathetic nerve activity (intraneural microelectrodes) increased to 220 +/- 28% of the room air baseline. Approximately two-thirds of this sympathetic activation persisted after return to room air breathing for the duration of our measurements (20 min in 8 subjects, 1 h in 2 subjects). In contrast, neither ventilation nor sympathetic outflow changed during time control experiments. A 20-min exposure to hyperoxic hypercapnia also caused a sustained increase in sympathetic activity, but, unlike the aftereffect of asphyxia, this effect was short lived and coincident with continued hyperpnea. In summary, relatively brief periods of asphyxic stimulation cause substantial increases in sympathetic vasomotor outflow that outlast the chemical stimuli. These findings provide a potential explanation for the chronically elevated sympathetic nervous system activity that accompanies sleep apnea syndrome. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT PREVENT MED,MADISON,WI 53706. UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,MADISON,WI 53706. RP MORGAN, BJ (reprint author), UNIV WISCONSIN,DEPT KINESIOL,5175 MED SCI CTR,1300 UNIV AVE,MADISON,WI 53706, USA. NR 37 TC 150 Z9 152 U1 0 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JUL PY 1995 VL 79 IS 1 BP 205 EP 213 PG 9 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA RK488 UT WOS:A1995RK48800031 PM 7559221 ER PT J AU SIMON, PM LEEVERS, AM MURTY, JL SKATRUD, JB DEMPSEY, JA AF SIMON, PM LEEVERS, AM MURTY, JL SKATRUD, JB DEMPSEY, JA TI NEUROMECHANICAL REGULATION OF RESPIRATORY MOTOR OUTPUT IN VENTILATOR-DEPENDENT C-1-C-3 QUADRIPLEGICS SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE PHRENIC AFFERENTS; CHEST WALL MECHANORECEPTORS; INSPIRATORY INHIBITION; MECHANICAL VENTILATION ID MECHANICAL VENTILATION; AIR HUNGER; INSPIRATORY ACTIVITY; MUSCLE-ACTIVITY; PRESSURE; HUMANS; INHIBITION; AFFERENTS; VOLUME; FREQUENCY AB To evaluate the role of phrenic and sternocleidomastoid afferents as alternate sources of inhibitory feedback. during mechanical ventilation, we studied five C-2-C-3 quadriplegics with sensory denervation of the rib cage and diaphragm, six C-1-C-2 quadriplegics with additional loss of sensory feedback from the neck muscles, and seven normal subjects. We compared the return of inspiratory muscle activity [the recruitment-threshold (PCO2RT)] during mechanical ventilation between subject groups after stepwise increases in end-tidal PCO2 (PET(CO2)) either by increasing the inspired fraction of CO2 (FICO2), decreasing tidal volume (VT; 50 ml/min), or decreasing frequency (f; 1 breath/2 min). Normal subjects were mechanically hyperventilated via a nasal mask until inspiratory activity was undetectable. Efferent input to the sternocleidomastoid was intact at both levels of spinal cord injury, but phasic activity was not evident at the quadriplegics' baseline resting ventilation. The PCO2RT was defined as the level of PET(CO2) at which phasic activity of the diaphragm in normal subjects and of the sternocleidomastoid in C-1-C-2 and C-2-C-3 quadriplegics recurred. The mean PCO2RT (in response to raising PET(CO2) via increased FICO2 while maintaining a high VT and f) was not significantly different (P = 0.6) between normal subjects (43 +/- 3 Torr) and C-2-C-3 quadriplegics (38 +/- 5 Torr). Both subject groups demonstrated a frequency- and volume-related inhibition, as evidenced by a substantially lower PCO2RT when PET(CO2) was raised by reducing either VT or f In contrast to the C-2-C-3 quadriplegics, the C-1-C-2 quadriplegics responded with a similar PCO2RT among the three different mechanical ventilation trials, independent-of whether PET(CO2) was raised with high VT and f, with reduced VT, or with reduced f. We conclude that feedback from at least some part of the chest wall is required to produce a volume- and frequency-dependent inhibition of inspiratory muscle activity observed during mechanical ventilation. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MED RES SERV,MADISON,WI 53705. UNIV WISCONSIN,DEPT MED,JOHN RANKIN LAB PULM MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT PREVENT MED,MADISON,WI 53706. RP SIMON, PM (reprint author), MAYO CLIN & MAYO FDN,DIV PULM & CRIT CARE MED,4-411 ALFRED BLDG,200 1ST ST SW,ROCHESTER,MN 55905, USA. NR 39 TC 10 Z9 10 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JUL PY 1995 VL 79 IS 1 BP 312 EP 323 PG 12 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA RK488 UT WOS:A1995RK48800045 PM 7559237 ER PT J AU REDDY, SV SINGER, FR ROODMAN, GD AF REDDY, SV SINGER, FR ROODMAN, GD TI BONE-MARROW MONONUCLEAR-CELLS FROM PATIENTS WITH PAGETS-DISEASE CONTAIN MEASLES-VIRUS NUCLEOCAPSID MESSENGER-RIBONUCLEIC-ACID THAT HAS MUTATIONS IN A SPECIFIC REGION OF THE SEQUENCE SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID RESPIRATORY SYNCYTIAL VIRUS; ANTIGENS; RNA; OSTEOCLASTS; INCLUSIONS; POLYMERASE; DNA AB Ultrastructural, immunocytochemical, and in situ hybridization studies have suggested that paramyxoviruses, such as measles virus (MV), are present in Pagetic osteoclasts and may contribute to the abnormality in osteoclast function. However, little additional information is known about potential viruses present in Pagetic osteoclasts. As there are increased numbers of osteoclast precursors among the marrow mononuclear cells of Paget's patients, we used the reverse transcriptase-polymerase chain reaction to amplify the nucleocapsid sequence of MV from freshly isolated bone marrow-derived mononuclear cells to examine the potential role of these viruses in cells in the osteoclast lineage. We detected MV nucleocapsid transcripts in 5 of 6 individual Paget's patients' marrow samples. MV transcripts were not detected in marrow samples from 10 normal subjects. Sequence analysis of the PCR products revealed that 1 patient had the same sequence as the Edmonston strain of MV. The remaining 4 patients had point mutations clustered between position 1360-1371 base pairs. Two of the patients exhibited identical mutations at this region. In total, 3 different point mutations were identified that resulted in amino acid substitutions. These data show that 1) unlike those from normal subjects, marrow mononuclear cells from Pager's patients express MV nucleocapsid messenger ribonucleic acid; and 2) mutations of a specific region of the MV nucleocapsid gene were present in 4 of 5 patients and suggest a persistent MV infection in Pagetic osteoclast precursors. These data further suggest that osteoclasts are infected by fusion with infected precursors. C1 VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, SAN ANTONIO, TX 78284 USA. JOHN WAYNE CANC INST, SANTA MONICA, CA 90404 USA. ST JOHNS HOSP, SANTA MONICA, CA 90404 USA. FU NIADDK NIH HHS [AM-35188]; NIAMS NIH HHS [AR-39539, AR-41336] NR 26 TC 73 Z9 74 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JUL PY 1995 VL 80 IS 7 BP 2108 EP 2111 DI 10.1210/jc.80.7.2108 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RP492 UT WOS:A1995RP49200022 PM 7608263 ER PT J AU WOLFE, JT CHOOBACK, L FINN, DT JAWORSKY, C ROOK, AH LESSIN, SR AF WOLFE, JT CHOOBACK, L FINN, DT JAWORSKY, C ROOK, AH LESSIN, SR TI LARGE-CELL TRANSFORMATION FOLLOWING DETECTION OF MINIMAL RESIDUAL DISEASE IN CUTANEOUS T-CELL LYMPHOMA - MOLECULAR AND IN-SITU ANALYSIS OF A SINGLE NEOPLASTIC T-CELL CLONE EXPRESSING THE IDENTICAL T-CELL RECEPTOR SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; MYCOSIS-FUNGOIDES; SEZARY-SYNDROME; LEUKEMIA AB Purpose: One of the unique characteristics of cutaneous T-cell lymphoma (CTCL) is its ability to undergo cytologic transformation in which the malignant T-cells develop the morphologic appearance of a large-cell lymphoma. Reported to occur in vp to 20% of advanced cases, large-cell transformation (LCT) is associated with an aggressive clinical course, Little is known about the risk factors or the molecular mechanisms of LCT. Before current immunohistochemical and molecular techniques, it was not possible to determine if LCT represented changes of the initial neoplastic T-cell clone or, in fact, was a distinct second malignancy. The goal of this study was to define the clonal evolution of LCT in CTCL. Patients and Method: Polymerase chain reaction (PCR) amplification of T-cell receptor-beta (TCR-beta) gene rearrangements and immunohistochemistry with monoclonol antibodies to TCR-V beta regions were used as markers of T-cell clonality to analyze the skin and peripheral blood of a patient with CTCL and LCT. Results: We first detected the presence of minimal residual disease (MRD) in a CTCL patient with a complete clinical response to biologic response modifiers (BRMs). When clinical relapse occurred and demonstrated LCT, TCR-beta-PCR and in situ immunohistochemistry with a specific TCR-V beta monoclonal antibody identified a single neoplastic T-cell clone that expressed the identical TCR as the original clone. Conclusion: Our results confirm a common clonal origin for CTCL and LCT. We also provide evidence of MRD in CTCL by molecular analysis, implying that residual malignant cells maintain a potential for clinical relapse and possibly LCT, The role of MRD detection remains to be defined in the clinical assessment of CTCL. LCT in CTCL provides a unique model to investigate the molecular events that underlie terminal-stage tumor progression. C1 PHILADELPHIA VET AFFAIRS MED CTR,MOLEC BIOL CORE FACIL,PHILADELPHIA,PA 19104. UNIV PENN,MED CTR,CUTANEOUS LYMPHOMA GRP,PHILADELPHIA,PA 19104. UNIV PENN,MED CTR,DEPT DERMATOL,PHILADELPHIA,PA 19104. FU NCI NIH HHS [R29 CA-55017, R01-CA-58841]; NIAMS NIH HHS [T32 AR07465] NR 17 TC 32 Z9 32 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JUL PY 1995 VL 13 IS 7 BP 1751 EP 1757 PG 7 WC Oncology SC Oncology GA RG157 UT WOS:A1995RG15700030 PM 7602365 ER PT J AU SILVA, JA LEONG, GB WEINSTOCK, R PENNY, G AF SILVA, JA LEONG, GB WEINSTOCK, R PENNY, G TI DANGEROUS DELUSIONS OF MISIDENTIFICATION OF THE SELF SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE PSYCHIATRY; PSYCHOSIS; DELUSIONS; DELUSIONAL MISIDENTIFICATION; AGGRESSION; VIOLENCE; DANGEROUSNESS ID CAPGRAS SYNDROME; SUBJECTIVE DOUBLES AB Delusions of misidentification of the self involve radical misidentification of physical and/or psychological aspects of the self. These delusions have received limited attention from a phenomenological as well as from a forensic psychiatric perspective. In this article we present a series of four cases of dangerous delusional misidentification of the self and discuss important factors that may contribute to their dangerousness. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 27 TC 8 Z9 8 U1 0 U2 1 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD JUL PY 1995 VL 40 IS 4 BP 570 EP 573 PG 4 WC Medicine, Legal SC Legal Medicine GA RL472 UT WOS:A1995RL47200011 PM 7595292 ER PT J AU LISH, JD ZIMMERMAN, M FARBER, NJ LUSH, D KUZMA, MA PLESCIA, G AF LISH, JD ZIMMERMAN, M FARBER, NJ LUSH, D KUZMA, MA PLESCIA, G TI PSYCHIATRIC SCREENING IN GERIATRIC PRIMARY-CARE - SHOULD IT BE FOR DEPRESSION ALONE SO JOURNAL OF GERIATRIC PSYCHIATRY AND NEUROLOGY LA English DT Review ID DIAGNOSTIC INTERVIEW SCHEDULE; ELDERLY MEDICAL INPATIENTS; NORMAL CORONARY-ARTERIES; CATCHMENT-AREA SITES; ATYPICAL CHEST PAIN; AGE CONCERN SURVEY; DSM-III-R; PANIC DISORDER; SOMATIZATION DISORDER; MENTAL-DISORDERS AB Depression in the elderly is highly prevalent, associated with functional disability and increased medical costs, and treatable; however, it is infrequently recognized and treated. The Agency for Health Care Policy and Research has advocated, therefore, increased case-finding efforts for depression in primary geriatric care. Anxiety, substance, and somatoform disorders in the elderly are similarly prevalent, associated with disability and cost, treatable, and also infrequently detected and treated. We believe that psychiatric case-finding in geriatric primary care should attend to these disorders, therefore, as well as to depression. In the present study, we examined whether the association between depressive and nondepressive forms of psychopathology was similar in geriatric and nongeriatric medical patients. We also examined the relationship between each type of pathology and health care utilization and global ratings of physical and mental health. In a VA hospital general medical outpatient clinic, 508 patients completed the SCREENER, which is a brief self-report questionnaire that screens for a range of psychiatric disorders, along with a self-report questionnaire regarding subjective health and medical care utilization. Of these patients, 98% were male, and the median age was 63 years. Patients aged 63 and over were compared to younger patients. In both geriatric and younger adult patients, we found substantial comorbidity between depressive and nondepressive forms of pathology. Moreover, in both age groups, there were significant associations between both depressive and nondepressive symptoms and fair-to-poor self-rated physical and mental health and increased medical care utilization. Approximately half of the cases of nondepressive disorders in the elderly were not comorbid with depression, and thus would not have been detected by screening for depression alone. Therefore, psychiatric case finding in primary care of geriatric males should be directed at anxiety, substance, and somatoform disorders, as well as at depression, for treatment resources to be triaged to maximally decrease morbidity and cost. C1 MED COLL PENN,DEPT PSYCHIAT,PHILADELPHIA,PA 19129. MED COLL PENN,DIV GEN MED,PHILADELPHIA,PA 19129. VET AFFAIRS MED CTR,DEPT INTERNAL MED,PHILADELPHIA,PA. NR 129 TC 8 Z9 8 U1 21 U2 21 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON ST, PO BOX 620, LCD 1, HAMILTON ON L8N 3K7, CANADA SN 0891-9887 J9 J GERIATR PSYCH NEUR JI J. Geriatr. Psychiatry Neurol. PD JUL PY 1995 VL 8 IS 3 BP 141 EP 153 PG 13 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA RJ669 UT WOS:A1995RJ66900001 PM 7576037 ER PT J AU BENNETT, CL HORNER, RD WEINSTEIN, RA KESSLER, HA DICKINSON, GM PITRAK, DL GILMAN, SC GEORGE, WL COHN, SE SIMBERKOFF, MS JACOBSON, JM DEHOVITZ, JA GOETZ, MB SHAPIRO, MF AF BENNETT, CL HORNER, RD WEINSTEIN, RA KESSLER, HA DICKINSON, GM PITRAK, DL GILMAN, SC GEORGE, WL COHN, SE SIMBERKOFF, MS JACOBSON, JM DEHOVITZ, JA GOETZ, MB SHAPIRO, MF TI EMPIRICALLY TREATED PNEUMOCYSTIS-CARINII PNEUMONIA IN LOS-ANGELES, CHICAGO, AND MIAMI - 1987-1990 SO JOURNAL OF INFECTIOUS DISEASES LA English DT Note ID IN-HOSPITAL MORTALITY; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; PNEUMONIA; AIDS; EXPERIENCE AB Many patients infected with the human immunodeficiency virus (HIV) with symptoms suggestive of pneumonia are treated empirically for Pneumocystis carinii pneumonia (PCP), although other bacterial infections (e.g., tuberculosis) and pulmonary Kaposi's sarcoma may cause identical symptoms. Empiric treatment for PCP may result in misdiagnosis and mistreatment. When the outcomes of cytologically confirmed versus empirically treated PCP cases were evaluated, the most important predictors of in-hospital mortality were severity of illness and use of bronchoscopy. Persons who did not undergo bronchoscopy had higher mortality rates than patients negative by bronchoscopy or cytologically confirmed as positive for PCP (22% vs. 11% vs. 14%, P < .01), although severity of illness and timing of anti-PCP medications did not differ significantly. Compared with cytologically confirmed cases persons who did not have bronchoscopy were more likely to die than were bronchoscopy-negative patients (P < .05), after adjusting for severity of illness. Bronchoscopy use may have contributed to better outcomes for persons treated for HIV-related PCP. C1 WESTSIDE VET AFFAIRS MED CTR,CHICAGO,IL. UNIV ILLINOIS,COOK CTY HOSP,MED CTR,CHICAGO,IL 60680. RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL. VET AFFAIRS MED CTR,DURHAM,NC 27705. DUKE UNIV,MED CTR,DURHAM,NC. VET AFFAIRS MED CTR,LONG BEACH,CA. UNIV CALIF IRVINE,MED CTR,LONG BEACH,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. VET AFFAIRS MED CTR,BRONX,NY. ALBERT EINSTEIN UNIV,MED CTR,BRONX,NY. SUNY HLTH SCI CTR,BROOKLYN,NY. MANHATTAN VET AFFAIRS MED CTR,NEW YORK,NY. UNIV ROCHESTER,MED CTR,ROCHESTER,NY 14642. VET AFFAIRS MED CTR,MIAMI,FL 33125. UNIV MIAMI,MED CTR,MIAMI,FL. NORTHWESTERN UNIV,MED CTR,CHICAGO,IL 60611. UNIV CALIF LOS ANGELES,MED CTR,LOS ANGELES,CA. NYU MED CTR,NEW YORK,NY. RP BENNETT, CL (reprint author), LAKESIDE VET AFFAIRS MED CTR,DEPT MED,111,333 E HURON ST,CHICAGO,IL 60611, USA. RI Bennett, Charles/C-2050-2008 OI Goetz, Matthew/0000-0003-4542-992X FU AHRQ HHS [IR01-HS06494, IR03-HSO7846-01] NR 12 TC 17 Z9 17 U1 0 U2 3 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD JUL PY 1995 VL 172 IS 1 BP 312 EP 315 PG 4 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA RF041 UT WOS:A1995RF04100055 PM 7797940 ER PT J AU LEVINE, RL TURSKI, PA GRIST, TM AF LEVINE, RL TURSKI, PA GRIST, TM TI BASILAR ARTERY DOLICHOECTASIA - REVIEW OF THE LITERATURE AND 6 PATIENTS STUDIED WITH MAGNETIC-RESONANCE ANGIOGRAPHY SO JOURNAL OF NEUROIMAGING LA English DT Article ID RESOLUTION COMPUTED-TOMOGRAPHY; VERTEBROBASILAR DOLICHOECTASIA; MEGADOLICHOBASILAR ARTERY; ECTASIA AB Six patients for whom computed tomography revealed a curvilinear calcific mass anterior to their brainstem were evaluated and magnetic resonance imaging and magnetic resonance angiography were performed on each. Magnetic resonance studies confirmed the suspicion of basilar artery dolichoectasia, and demonstrated a partial thrombus in the basilar artery in 1 patient. The patients' clinical features were combined with those of basilar artery dolichoectasia patients reported in the literature (n = 122) who had case histories sufficiently detailed enough to determine each person's mode of clinical presentation. Basilar artery dolichoectasia patients were more often men (95/128, 74%) and had a mean age of 59 +/- 11 years. Of the 128 patients studied, there were cranial nerve compressive signs in 74 (58%), especially facial spasm (29/74, 39%) and trigeminal neuralgia (20/74, 27%); vertebral basilar insufficiency or vertebral basilar stroke or both in 61 (48%); hydrocephalus in 40 (31%); compressive brainstem symptoms and signs that progressed clinically in 31 (24%); and arterial hypertension in 31 (24%). Magnetic resonance imaging and magnetic resonance angiography safely diagnose this interesting arterial abnormality. The modes of clinical presentation of this disorder are reviewed. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT NEUROL,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,MADISON,WI 53705. UNIV WISCONSIN,DEPT RADIOL,MADISON,WI 53706. NR 33 TC 21 Z9 27 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 1051-2284 J9 J NEUROIMAGING JI J. Neuroimaging PD JUL PY 1995 VL 5 IS 3 BP 164 EP 170 PG 7 WC Clinical Neurology; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA RJ936 UT WOS:A1995RJ93600006 PM 7626824 ER PT J AU ATKINSON, SE WILSON, PW AF ATKINSON, SE WILSON, PW TI COMPARING MEAN EFFICIENCY AND PRODUCTIVITY SCORES FROM SMALL SAMPLES - A BOOTSTRAP METHODOLOGY SO JOURNAL OF PRODUCTIVITY ANALYSIS LA English DT Article DE BOOTSTRAP; DATA ENVELOPMENT ANALYSIS; FIXED AND RANDOM EFFECTS; MALMQUIST INDEX; PRODUCTIVITY MEASUREMENT; SMALL SAMPLE; PANEL DATA ID CONFIDENCE-INTERVALS; PANEL DATA AB This paper provides a bootstrap methodology for constructing confidence intervals for means of DEA and econometrically estimated efficiency scores, Malmquist productivity indices, and other similar measures in small samples. The procedure is nonparametric since no distributional assumptions are required. An empirical example is provided. C1 UNIV TEXAS,DEPT ECON,AUSTIN,TX 78712. US DEPT VET AFFAIRS,MANAGEMENT SCI GRP,BEDFORD,MA 01730. RP ATKINSON, SE (reprint author), UNIV GEORGIA,DEPT ECON,ATHENS,GA 30602, USA. NR 25 TC 49 Z9 49 U1 1 U2 4 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0895-562X J9 J PROD ANAL JI J. Prod. Anal. PD JUL PY 1995 VL 6 IS 2 BP 137 EP 152 DI 10.1007/BF01073408 PG 16 WC Business; Economics; Social Sciences, Mathematical Methods SC Business & Economics; Mathematical Methods In Social Sciences GA RL083 UT WOS:A1995RL08300003 ER PT J AU BOHN, MJ BABOR, TF KRANZLER, HR AF BOHN, MJ BABOR, TF KRANZLER, HR TI THE ALCOHOL-USE DISORDERS IDENTIFICATION TEST (AUDIT) - VALIDATION OF A SCREENING INSTRUMENT FOR USE IN MEDICAL SETTINGS SO JOURNAL OF STUDIES ON ALCOHOL LA English DT Article ID COLLABORATIVE PROJECT; CAGE QUESTIONNAIRE; CONSUMPTION; INDICATORS; DIAGNOSIS; DRINKING; SAMPLE AB Objective: The concurrent, construct, and discriminant validity of the Alcohol Use Disorders Identification Test (AUDIT) were evaluated. AUDIT consists of a 10-item Core questionnaire and an 8-item Clinical procedure. AUDIT was designed to identify hazardous drinkers (whose drinking increases their risk of alcohol-related problems, though alcohol-associated harm has not yet occurred); harmful drinkers (who have had recent physical or mental harm from their drinking, but who are not alcohol-dependent); and people with alcohol dependence. Method: Known alcoholics (n = 65) and general medical patients (n = 187) completed self-report questionnaires and underwent a diagnostic interview, physical examination and laboratory testing. Results: AUDIT scores correlated significantly with scores on the MAST and MacAndrew alcoholism screening tests, and with ALAT, ASAT, GGT and MCV levels, which reflect recent heavy drinking. AUDIT scores were correlated with measures of alcoholism vulnerability (e.g., familial alcoholism and sociopathy), and with somatic and affective consequences of drinking. Receiver operating characteristic and discriminant function analyses indicated that the AUDIT Core and Clinical Instruments were sensitive and specific in discriminating alcoholics from medical patients, most of whom were nonalcoholics. The AUDIT Core was superior to the MAST and the AUDIT Clinical in discriminating hazardous drinkers from nonhazardous drinkers. It was also superior to the AUDIT Clinical in discriminating harmful from nonharmful drinkers. Conclusions: The AUDIT Core Instrument is useful for early detection of hazardous or harmful drinking, while the AUDIT Clinical Instrument is better applied to identification and/or confirmation of cases of alcohol dependence. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. RP BOHN, MJ (reprint author), UNIV WISCONSIN,SCH MED,CTR CLIN SCI,DEPT PSYCHIAT,B6-210,MADISON,WI 53792, USA. FU NIAAA NIH HHS [P50-AA03510, R29-AA09948, T32-AA07290] NR 45 TC 426 Z9 436 U1 3 U2 18 PU ALCOHOL RES DOCUMENTATION INC CENT ALCOHOL STUD RUTGERS UNIV PI PISCATAWAY PA PO BOX 969, PISCATAWAY, NJ 08855-0969 SN 0096-882X J9 J STUD ALCOHOL JI J. Stud. Alcohol PD JUL PY 1995 VL 56 IS 4 BP 423 EP 432 PG 10 WC Substance Abuse; Psychology SC Substance Abuse; Psychology GA RF318 UT WOS:A1995RF31800009 PM 7674678 ER PT J AU GAUMNITZ, E SWEET, MA SENGUPTA, A SINGARAM, C AF GAUMNITZ, E SWEET, MA SENGUPTA, A SINGARAM, C TI NITRINERGIC AND PEPTIDERGIC INNERVATIONS AND THEIR INTERRELATIONSHIPS IN HUMAN COLON SO NEUROPEPTIDES LA English DT Article ID NITRIC-OXIDE SYNTHASE; NEURONAL NADPH DIAPHORASE; SMOOTH-MUSCLE; HUMAN GUT; INVOLVEMENT; IMMUNOREACTIVITY; INTESTINE; VIP AB The distribution and colocalization of nitrinergic and peptidergic nerves were examined in six human colons. The tissues were fixed, cryosectioned, and standard immunohistochemistry was performed for several known neuropeptides. The same sections were stained for NADPH-diaphorase to denote nitric oxide synthase. NADPH-diaphorase-positive myenteric neurons were counted and colocalization noted for each peptide, as well as for peptide terminations. Galanin was the only neuropeptide that colocalized to a significant extent (23.0 +/- 7.21%) with NADPH-diaphorase-positive myenteric neurons. Many neuropeptide-containing nerve fibers had extensive terminations onto NADPH-diaphorase-positive neurons. Vasoactive intestinal peptide was the only neuropeptide that colocalized with NADPH-diaphorase to any extent in nerve fibers within circular muscle (59.5 +/- 9.3%). Fiber distribution in the longitudinal muscles showed a similar, but less dense pattern. These observations provide morphological evidence for the presence of nitric oxide, a candidate nonadrenergic noncholinergic neurotransmitter in the human colon. C1 UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,DEPT MED,DIV GASTROENTEROL,MADISON,WI. BOSTON UNIV,SCH MED,DIV PEDIAT GASTROENTEROL & NUTR,BOSTON,MA. NR 24 TC 18 Z9 19 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH, MIDLOTHIAN, SCOTLAND EH1 3AF SN 0143-4179 J9 NEUROPEPTIDES JI Neuropeptides PD JUL PY 1995 VL 29 IS 1 BP 1 EP 9 DI 10.1016/0143-4179(95)90050-0 PG 9 WC Endocrinology & Metabolism; Neurosciences SC Endocrinology & Metabolism; Neurosciences & Neurology GA RH553 UT WOS:A1995RH55300001 PM 7566507 ER PT J AU STREIM, JE AF STREIM, JE TI OBRA REGULATIONS AND PSYCHIATRIC-CARE IN THE NURSING-HOME SO PSYCHIATRIC ANNALS LA English DT Article ID FACILITIES; RESTRAINTS; IMPACT C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP STREIM, JE (reprint author), UNIV PENN,DEPT PSYCHIAT,GERIATR PSYCHIAT SECT,RALSTON PENN CTR,3615 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. NR 29 TC 10 Z9 10 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 0048-5713 J9 PSYCHIAT ANN JI Psychiatr. Ann. PD JUL PY 1995 VL 25 IS 7 BP 413 EP 418 PG 6 WC Psychiatry SC Psychiatry GA RH497 UT WOS:A1995RH49700006 ER PT J AU Horstman, DH Ball, BA Brown, J Gerrity, T Folinsbee, LJ AF Horstman, DH Ball, BA Brown, J Gerrity, T Folinsbee, LJ TI Comparison of pulmonary responses of asthmatic and nonasthmatic subjects performing light exercise while exposed to a low level of ozone SO TOXICOLOGY AND INDUSTRIAL HEALTH LA English DT Article DE asthma; light exercise; ozone; prolonged exposure; pulmonary function ID HOSPITAL ADMISSIONS; AIR-POLLUTION; MODERATE EXERCISE; SOUTHERN ONTARIO; UNITED-STATES; 0.12 PPM; HEALTHY AB To determine if asthmatic subjects (ASTH, n = 17) experience greater O-3-induced pulmonary decrements than nonasthmatic subjects (NONA, n = 13), both groups were exposed for 7.6 h to both clean air and 0.16 ppm O-3. Exposures consisted of seven 50-min periods of light exercise (V-E = 14.2 and 15.3 l/min/m(2) for ASTH and NONA, respectively), each followed by 10 min rest. A 35-min lunch period followed the third exercise. Following O-3 exposure, decrements in forced expiratory volume in one second (FEV(1)) and FEV(1) divided by forced viral capacity (FVC), corrected for air exposure, for ASTH (-19.4 +/- 3.1 % and 6.2 +/- 2%, respectively) were significantly greater (p = 0.04 and 0.02) than for NONA (-9.8 +/- 1.9% and -1 +/- 1%, respectively). There was no difference (p = 0.33) for decrements in FVC between ASTH (-11.8 +/- 1.9%) and NONA (-8.8 +/- 2.1%). Nine of 17 ASTH experienced wheezing with O-3, while only one experienced wheezing with air (p = 0.004); no NONA experienced wheezing. Six of 17 ASTH requested inhaled P-agonist bronchodilator prior to and/or during O-3 exposure and experienced some temporary alleviation of decrements. At end exposure, however, ASTH who were medicated had greater Os-induced decrements than those who were not medicated. ASTH who had the larger Os-induced decrements had lower baseline FEV(1)/FVC and lower baseline %predicted FEV(1). These data indicate that in ASTH, unlike NONA, some portion of O-3-induced pulmonary decrements experienced was related to bronchoconstriction, and that O-3-responsiveness for ASTH depended upon baseline airway status. C1 UNIV N CAROLINA,CTR ENVIRONM MED & LUNG BIOL,CHAPEL HILL,NC. US DEPT VET AFFAIRS,MED RES SERV,WASHINGTON,DC. RP Horstman, DH (reprint author), US EPA,NATL HLTH & ENVIRONM EFFECTS RES LAB,DIV HUMAN STUDIES,CLIN RES BRANCH MD58B,RES TRIANGLE PK,NC 27711, USA. NR 24 TC 35 Z9 36 U1 1 U2 3 PU PRINCETON SCIENTIFIC PUBL INC PI PRINCETON PA PO BOX 2155, PRINCETON, NJ 08543 SN 0748-2337 J9 TOXICOL IND HEALTH JI Toxicol. Ind. Health PD JUL-AUG PY 1995 VL 11 IS 4 BP 369 EP 385 PG 17 WC Public, Environmental & Occupational Health; Toxicology SC Public, Environmental & Occupational Health; Toxicology GA TK629 UT WOS:A1995TK62900001 PM 8748419 ER PT J AU FILLEY, CM KLEINSCHMIDTDEMASTERS, BK AF FILLEY, CM KLEINSCHMIDTDEMASTERS, BK TI NEUROBEHAVIORAL PRESENTATIONS OF BRAIN NEOPLASMS SO WESTERN JOURNAL OF MEDICINE LA English DT Article ID TEMPORAL-LOBE; BEHAVIOR; DISORDER; DISEASE AB We studied 8 patients with frontal or temporolimbic neoplasms who had psychiatric presentations to clarify diagnostic criteria for distinguishing psychiatric disease from structural brain lesions and to examine brain-behavior relationships associated with cerebral neoplasms using modern neuroimaging techniques. Medical records were retrospectively reviewed for evidence of neurobehavioral and neurologic manifestations, tumor histologic features, and the results of treatment. Clinical presentations were correlated with tumor location as determined by computed tomography and magnetic resonance imaging. Patients with frontal lobe tumors presented with abulia, personality change, or depression, whereas those with temporolimbic tumors had auditory and visual hallucinations, mania, panic attacks, or amnesia. After treatment, neurobehavioral syndromes abated or resolved in 7 of 8 patients. We recommend that any patient 40 years of age or older with a change in mental state, cognitive or emotional, should have neuroimaging of the brain. Any patient with a psychiatric presentation who has specific neurobehavioral or neurologic findings or an unexpectedly poor response to psychopharmacologic treatment should also have brain imaging. These case reports extend and update observations on the importance of frontal and temporolimbic systems in the pathogenesis of neurobehavioral disorders. C1 UNIV COLORADO,HLTH SCI CTR,DEPT PATHOL,DENVER,CO 80262. UNIV COLORADO,HLTH SCI CTR,DEPT PSYCHIAT,DENVER,CO 80262. DENVER VET AFFAIRS MED CTR,DENVER,CO. RP FILLEY, CM (reprint author), UNIV COLORADO,HLTH SCI CTR,DEPT NEUROL,BEHAV NEUROL SECT,4200 E 9TH AVE,DENVER,CO 80262, USA. NR 22 TC 42 Z9 44 U1 0 U2 1 PU CALIF MEDICAL ASSN PI SAN FRANCISCO PA 221 MAIN STREET, SAN FRANCISCO, CA 94105 SN 0093-0415 J9 WESTERN J MED JI West. J. Med. PD JUL PY 1995 VL 163 IS 1 BP 19 EP 25 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA RL563 UT WOS:A1995RL56300001 PM 7667978 ER PT J AU WILLIAMS, JW AF WILLIAMS, JW TI SINUSITIS - BEGINNING A NEW-AGE OF ENLIGHTENMENT SO WESTERN JOURNAL OF MEDICINE LA English DT Editorial Material C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP WILLIAMS, JW (reprint author), UNIV TEXAS,HLTH SCI CTR,DIV GEN INTERNAL MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. RI Williams, Jr., John/A-3696-2008 OI Williams, Jr., John/0000-0002-5267-5558 NR 4 TC 4 Z9 4 U1 0 U2 0 PU CALIF MEDICAL ASSN PI SAN FRANCISCO PA 221 MAIN STREET, SAN FRANCISCO, CA 94105 SN 0093-0415 J9 WESTERN J MED JI West. J. Med. PD JUL PY 1995 VL 163 IS 1 BP 80 EP 82 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA RL563 UT WOS:A1995RL56300021 PM 7667997 ER PT J AU MULROW, CD WILLIAMS, JW GERETY, MB RAMIREZ, G MONTIEL, OM KERBER, C AF MULROW, CD WILLIAMS, JW GERETY, MB RAMIREZ, G MONTIEL, OM KERBER, C TI CASE-FINDING INSTRUMENTS FOR DEPRESSION IN PRIMARY-CARE SETTINGS SO ANNALS OF INTERNAL MEDICINE LA English DT Article DE DEPRESSION; PRIMARY HEALTH CARE; MASS SCREENING; PHYSICIANS, FAMILY; SENSITIVITY AND SPECIFICITY ID DIAGNOSTIC INTERVIEW SCHEDULE; GENERAL HEALTH QUESTIONNAIRE; PRIMARY MEDICAL-CARE; MENTAL-ILLNESS; RECOGNITION; PHYSICIANS; PREVALENCE; MANAGEMENT; DISORDERS; ANXIETY AB Objective: To evaluate the usefulness of case-finding instruments for identifying patients with major depression in primary care settings. Data Sources: A MEDLINE search of the English-language medical literature; bibliographies of selected papers; and experts. Study Selection: Studies that were done in primary care settings with unselected patients and that compared case-finding instruments with accepted diagnostic criterion standards for major depression were selected. Data Synthesis: 9 case-finding instruments were assessed in 18 studies. More than 15000 patients received screening with a case-finding instrument; approximately 5300 of these received criterion standard assessment. Case-finding instruments ranged in length from 2 to 28 questions. Average administration times ranged from less than 2 minutes to 6 minutes. Sensitivities and specificities for detecting major depression ranged from 67% to 99% and from 40% to 95%, respectively. No significant differences between instruments were found. Overall sensitivity was 84% (95% CI, 79% to 89%); overall specificity was 72% (CI, 67% to 77%). If a case-finding instrument were administered to 100 primary care patients with a 5% prevalence of major depression, the clinician could expect that 31 patients would screen positive, that 4 of the 31 would have major depression, and that 1 patient with major depression would not be identified. Conclusions: Several instruments with reasonable operating characteristics are available to help primary care clinicians identify patients with major depression. Because the operating characteristics of these instruments are similar, selection of a particular instrument should depend on issues such as feasibility, administration and scoring times, and the instruments' ability to serve additional purposes, such as monitoring severity or response to therapy. RP MULROW, CD (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,11C6,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. RI Williams, Jr., John/A-3696-2008 OI Williams, Jr., John/0000-0002-5267-5558 NR 64 TC 337 Z9 340 U1 5 U2 7 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUN 15 PY 1995 VL 122 IS 12 BP 913 EP 921 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA RC270 UT WOS:A1995RC27000004 PM 7755226 ER PT J AU CUMMINGS, JL AF CUMMINGS, JL TI DEMENTIA - THE FAILING BRAIN SO LANCET LA English DT Article C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,BEHAV NEUROSCI SECT,LOS ANGELES,CA 90073. RP CUMMINGS, JL (reprint author), UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT NEUROL,710 WESTWOOD PLAZA,LOS ANGELES,CA 90024, USA. FU NIA NIH HHS [AG 10123] NR 0 TC 25 Z9 26 U1 0 U2 0 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0099-5355 J9 LANCET JI Lancet PD JUN 10 PY 1995 VL 345 IS 8963 BP 1481 EP 1484 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA RC189 UT WOS:A1995RC18900011 PM 7769904 ER PT J AU FEIGIN, AM TEETER, JH BRAND, JG AF FEIGIN, AM TEETER, JH BRAND, JG TI THE INFLUENCE OF STEROLS ON THE SENSITIVITY OF LIPID BILAYERS TO MELITTIN SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID CHOLESTEROL; MEMBRANES; CHANNELS; PERMEABILITY; CONDUCTANCE; VESICLES AB The sensitivity of planar lipid bilayers to the permeabalizing effect of melittin was evaluated when sterols of varying structure were incorporated into the membrane. The addition of increasing amount,of cholesterol (0-50 mole %) decreased the sensitivity of membranes formed from negatively charged phospholipids to melittin but did not (in amount of up to 66 mole %) change the sensitivity of membranes formed from zwitterionic lipids. 7-Dehydrocholesterol, stigmasterol and ergosterol had the same ability as that of cholesterol to decrease the membrane sensitivity to melittin, while lanosterol had no effect on the sensitivity of membranes to melittin, The results suggest that the effect of sterols is complex and cannot be explained only by a direct interaction of melittin with cholesterol, by a decrease of membrane fluidity, or by changes in distribution of surface charge. (C) 1995 Academic Press, Inc. C1 UNIV PENN,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP FEIGIN, AM (reprint author), MONELL CHEM SENSES CTR,3500 MARKET ST,PHILADELPHIA,PA 19104, USA. NR 23 TC 14 Z9 14 U1 0 U2 3 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JUN 6 PY 1995 VL 211 IS 1 BP 312 EP 317 DI 10.1006/bbrc.1995.1812 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA RB850 UT WOS:A1995RB85000044 PM 7779101 ER PT J AU CHIAPPELLI, F GOTTESFELD, Z AF CHIAPPELLI, F GOTTESFELD, Z TI INTRODUCTION TO THE SYMPOSIUM SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Editorial Material ID FETAL ALCOHOL SYNDROME; PROLIFERATIVE RESPONSE; ETHANOL INUTERO; EXPOSURE ALTERS; LYMPHOID ORGANS; LIVER-DISEASE; IMMUNE; CELLS; SUPPRESSION; EXPRESSION C1 W LOS ANGELES VET AFFAIRS MED CTR,HUMAN IMMUNOL & PSYCHONEUROIMMUNOL LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024. UNIV TEXAS,SCH MED,DEPT ANAT & NEUROBIOL,HOUSTON,TX. RP CHIAPPELLI, F (reprint author), UNIV CALIF LOS ANGELES,SCH MED,SCH DENT,DIV DIAGNOST SCI,HUMAN ORAL & MOLEC IMMUNOL LAB,LOS ANGELES,CA 90095, USA. FU NIDA NIH HHS [DA07683] NR 56 TC 4 Z9 4 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD JUN PY 1995 VL 19 IS 3 BP 535 EP 538 DI 10.1111/j.1530-0277.1995.tb01544.x PG 4 WC Substance Abuse SC Substance Abuse GA RE228 UT WOS:A1995RE22800001 PM 7573770 ER PT J AU CHIAPPELLI, F KUNG, M LEE, P PHAM, L MANFRINI, E VILLANUEVA, P AF CHIAPPELLI, F KUNG, M LEE, P PHAM, L MANFRINI, E VILLANUEVA, P TI ALCOHOL MODULATION OF HUMAN NORMAL T-CELL ACTIVATION, MATURATION, AND MIGRATION SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE LYMPHOCYTES; T-CELL ACTIVATION; T-CELL MIGRATION; CD26; CD62L ID DIPEPTIDYL PEPTIDASE-IV; LYMPHOCYTES-T; EXPRESSION; ETHANOL; CD26 AB We are interested in the characterization of the effects of alcohol on human T-cell activation, maturation, and migration, because this cell population is crucial in the initiation, regulation, and propagation of cellular immunity. We and others have described the effects of both acute and chronic exposure of human immune cells to ethanol (EtOH) in vitro. Herein, we briefly, review these reports and expand this body of literature with the inclusion of new data recently obtained in our laboratory. We confirm the blunting effects of EtOH on the production of interleukin-2 and mitogen proliferative response following T-cell mitogen stimulation, and on the expression of membrane markers of activation. We show that EtOH significantly alters the expression of the CD4 cell-associated marker of activation, CD26. We report the effect of EtOH on the expression of the homing receptor CD62L by CD4(+) cells, and on their ability to adhere by a CD18-mediated process to a defined cellular substratum. Furthermore, we demonstrate the effects of EtOH and EtOH and beta-endorphin pretreatment on the activation of CD4(+) lymphocytes endowed with the homing receptor CD62L. C1 W LOS ANGELES VET AFFAIRS MED CTR, HUMAN IMMUNOL & PSYCHONEUROIMMUNOL LAB, LOS ANGELES, CA USA. UNIV CALIF LOS ANGELES, DEPT ANAT & CELL BIOL, LOS ANGELES, CA 90024 USA. RP CHIAPPELLI, F (reprint author), UNIV CALIF LOS ANGELES, SCH DENT, DIV DIAGNOST SCI, HUMAN ORAL & MOLEC IMMUNOL LAB, CHS 63-090, LOS ANGELES, CA 90095 USA. FU NIDA NIH HHS [DA07683] NR 31 TC 36 Z9 36 U1 0 U2 1 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD JUN PY 1995 VL 19 IS 3 BP 539 EP 544 DI 10.1111/j.1530-0277.1995.tb01545.x PG 6 WC Substance Abuse SC Substance Abuse GA RE228 UT WOS:A1995RE22800002 PM 7573771 ER PT J AU TAYLOR, AN TIO, DL CHIAPPELLI, F AF TAYLOR, AN TIO, DL CHIAPPELLI, F TI FETAL ALCOHOL AND THYMOCYTE PHENOTYPES IN OFFSPRING - RESPONSE TO FOOD-DEPRIVATION SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE FETAL ALCOHOL EXPOSURE; THYMUS; THYMOCYTES; FOOD RESTRICTION; PLASMA CORTICOSTERONE ID DIETARY RESTRICTION; PROLIFERATIVE RESPONSE; ETHANOL EXPOSURE; RATS; EXPRESSION; INUTERO; STRESS; CELLS AB Restriction of food availability is a reliable stimulus that leads to significant hypothalamo-pituitary-adrenal (HPA) activation to which rats do not habituate. Based on our previous data that indicated that the HPA response to some, but not all, stressful stimuli is significantly greater in adult offspring of Sprague-Dawley darns exposed to 35% alcohol during the last 2 weeks of gestation than that of control rats and on the mounting neuroendocrine-immune literature that describes the role of pituitary-adrenal products in modulating cellular immunity, we hypothesized that the outcomes of food restriction would be significantly more marked in fetal alcohol-exposed (FAE) offspring, compared with control rats. Data we report herein show that-whereas food restriction at 30-35 days of age produced significant changes in body weight, thymus weight-to-body weight ratio, adrenal weight-to-body weight ratio, plasma corticosterone revels, and in thymocyte number, as well as in the percentage and absolute number of CD4(+) and CD8(+) thymocytes that express CD45RC-FAE and control rats were equally affected. We conclude that food restriction is another example of a stressful stimulus that fails to distinguish satisfactorily between FAE and control rats of prepubertal age. C1 UNIV CALIF LOS ANGELES,SCH MED,PSYCHONEUROIMMUNOL PROGRAM,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,ALCOHOL RES LAB,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,HUMAN IMMUNOL & PSYCHONEUROIMMUNOL LAB,LOS ANGELES,CA. RP TAYLOR, AN (reprint author), UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024, USA. NR 28 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD JUN PY 1995 VL 19 IS 3 BP 545 EP 550 DI 10.1111/j.1530-0277.1995.tb01546.x PG 6 WC Substance Abuse SC Substance Abuse GA RE228 UT WOS:A1995RE22800003 PM 7573772 ER PT J AU BOHN, MJ KRAHN, DD STAEHLER, BA AF BOHN, MJ KRAHN, DD STAEHLER, BA TI DEVELOPMENT AND INITIAL VALIDATION OF A MEASURE OF DRINKING URGES IN ABSTINENT ALCOHOLICS SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE ALCOHOLISM; URGES; CRAVING; QUESTIONNAIRE ID COMPULSIVE CHARACTERISTICS; DEPENDENCE; ABUSE; QUESTIONNAIRE; NALTREXONE AB Although drinking urges and cravings are commonly reported by alcoholics, prospective studies have found inconsistent associations between such urges and drinking relapses. Previous studies have measured drinking urges by use of single-item ratings of alcohol craving or other measures of unknown reliability and validity. To permit improved evaluation of hypotheses regarding alcohol craving, a 49-item questionnaire that reflects several urge-related domains was developed and pretested. items assessed subjects' desire for a drink, expectations of positive effects following drinking, relief of withdrawal and negative affect following drinking, and intention to drink. Exploratory and confirmatory factor analyses of the responses of 351 abstinent, treatment-seeking alcoholics indicated that alcohol urges are best described by a single factor. Based on these analyses, an internally consistent, reliable, and psychometrically valid 8-item scale, the Alcohol Urge Questionnaire (AUG), was developed. Data indicated that AUQ scores were strongly related to alcohol dependence severity and to cognitive preoccupation with alcohol, and that they declined with prolonged abstinence. The AUQ may be useful in alcoholism treatment research and in laboratory studies of reactivity to alcohol or other manipulations. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,PSYCHIAT SERV,MADISON,NJ. WILLIAM S MIDDLETON MEM VET ADM MED CTR,OUTPATIENT SUBSTANCE ABUSE TREATMENT PROGRAM,MADISON,NJ. RP BOHN, MJ (reprint author), UNIV WISCONSIN,SCH MED,DEPT PSYCHIAT,MADISON,WI 53792, USA. FU NIAAA NIH HHS [R29-AA09948-0] NR 39 TC 273 Z9 275 U1 0 U2 16 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0145-6008 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD JUN PY 1995 VL 19 IS 3 BP 600 EP 606 DI 10.1111/j.1530-0277.1995.tb01554.x PG 7 WC Substance Abuse SC Substance Abuse GA RE228 UT WOS:A1995RE22800011 PM 7573780 ER PT J AU DELPRATO, S RICCIO, A DEKREUTZENBERG, SV DORELLA, M TIENGO, A DEFRONZO, RA AF DELPRATO, S RICCIO, A DEKREUTZENBERG, SV DORELLA, M TIENGO, A DEFRONZO, RA TI BASAL PLASMA-INSULIN LEVELS EXERT A QUALITATIVE BUT NOT QUANTITATIVE EFFECT ON GLUCOSE-MEDIATED GLUCOSE-UPTAKE SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE HYPERGLYCEMIA; INSULINOPENIA; GLYCOLYSIS; INTERMEDIARY METABOLISM ID CONSCIOUS DOG; HYPERGLYCEMIA; HUMANS; METABOLISM; OXIDATION; DISPOSAL; RATES; SECRETION; KINETICS; INFUSION AB We assessed the effect of hyperglycemia on glucose uptake in the presence of normal basal insulin levels or somatostatin-induced hypoinsulinemia in seven normal volunteers during a 200-min hyperglycemic clamp (+9 mmol/l) carried out with [3-H-3]glucose and indirect calorimetry. Hyperglycemia increased glucose uptake to 22.4 +/- 2.6 and 21.3 +/- 1.6 mu mol . kg(-1). min(-1) with and without insulin replacement, respectively. Normoinsulinemia increased glucose oxidation (Delta = +4.5 +/- 0.6 mu mol . kg(-1). min(-1)) and nonoxidative glucose metabolism (Delta = +5.2 +/- 1.7 mu mol . kg(-1). min(-1)), whereas with insulinopenia, glucose oxidation did not change (Delta = -0.3 +/- 0.6 mu mol . kg(-1). min(-1)), and nonoxidative glucose metabolism increased (Delta = +8.7 +/- 0.8 mu mol . kg(-1). min(-1)). Nonoxidative glucose metabolism was higher during insulinopenic (13.5 +/- 1.8 mu mol . kg(-1). min(-1)) than normoinsulinemic hyperglycemia (9.8 +/- 2.7 mu mol . kg(-1). min(-1); P < 0.01). Plasma FFA concentration and lipid oxidation were higher with insulinopenia. Blood lactate and alanine concentrations were greater with normoinsulinemia. In conclusion: I)hyperglycemia promotes glucose uptake by stimulating both nonoxidative and oxidative glucose disposal; 2) the ability of hyperglycemia to enhance total body glucose uptake is similar with and without normoinsulinemia; 3) although acute insulinopenia does not impair the ability of hyperglycemia to stimulate glucose uptake, it plays a critical role in determining the intracellular metabolic fate of glucose taken up in response to hyperglycemia. C1 UNIV TEXAS, HLTH SCI CTR, DIV DIABET, SAN ANTONIO, TX 78284 USA. AUDIE L MURPHY VET AFFAIRS HOSP, SAN ANTONIO, TX 78284 USA. RP DELPRATO, S (reprint author), UNIV PADUA, CATTEDRA MALATTIE METAB, VIA GIUSTINIANI 2, I-35128 PADUA, ITALY. NR 31 TC 21 Z9 21 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN PY 1995 VL 268 IS 6 BP E1089 EP E1095 PG 7 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA RE366 UT WOS:A1995RE36600009 ER PT J AU YANG, H TACHE, Y AF YANG, H TACHE, Y TI PYY IN BRAIN-STEM NUCLEI INDUCES VAGAL-STIMULATION OF GASTRIC-ACID SECRETION IN RATS SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE THYROTROPIN-RELEASING HORMONE; DORSAL MOTOR NUCLEUS OF THE VAGUS; RAPHE PALLIDUS; RAPHE OBSCURUS; NUCLEUS AMBIGUUS; SEROTONIN; PREPRO-THYROTROPIN-RELEASING HORMONE-(160--169); (+/-)-1-(4-METHYL-1-PIPERAZINYL)-PYRROLO(1,2-A)QUINOXALINE; VAGUS ID THYROTROPIN-RELEASING-HORMONE; MEDULLARY RAPHE NUCLEI; PEPTIDE-YY; RECEPTOR-BINDING; TRH ANALOG; COMPLEX; SEROTONIN; SITES; NEURONS; PROJECTIONS AB The influence of peptide YY (PYY) microinjected into brain stem nuclei on gastric acid secretion (GAS) was investigated in urethan-anesthetized rats with gastric cannula. PYY (30-200 ng) microinjected into the dorsal motor nucleus of the vagus (DMN) induces a dose-related and vagal-dependent stimulation of GAS (net increase from 13 +/- 4 to 59 +/- 12 mu mol/90 min). PYY (200 ng) injected intravenously or intracisternally into sites adjacent to the DMN had no effect. GAS induced by PYY into the DMN was potentiated by coinjection of thyrotropin-releasing hormone (TRH, 30 ng) or the serotonin receptor (5-HT2) agonist (+/-)-1-(4-methyl-1-piperazinyl)-pyrrolo(1,2-a)quinoxaline (357 ng) and by microinjection of kainic acid (1 ng) into the raphe pallidus. Prepro-TRH-(160-169) (200 ng into the DMN) did not influence the stimulatory effect of PYY. PYY (200 ng) microinjected into the raphe pallidus, raphe obscurus, and nucleus ambiguus also increased GAS, although the response was of shorter duration than that in the DMN. These results indicate that PYY acts in brain stem nuclei involved in the vagal regulation of GAS and that PYY action in the DMN is potentiated by TRH or 5-HT2 receptor agonist acting at this site. C1 UNIV CALIF LOS ANGELES, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, BRAIN RES INST, LOS ANGELES, CA 90073 USA. RP YANG, H (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, CTR ULCER RES & EDUC, CTR GASTROENTER BIOL, BLDG 115, RM 207, LOS ANGELES, CA 90073 USA. FU NIDDK NIH HHS [DK-30110]; NIMH NIH HHS [MH-00663] NR 40 TC 31 Z9 31 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD JUN PY 1995 VL 268 IS 6 BP G943 EP G948 PG 6 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA RF527 UT WOS:A1995RF52700009 PM 7611415 ER PT J AU KATO, S IVESTER, CT COOPER, G ZILE, MR MCDERMOTT, PJ AF KATO, S IVESTER, CT COOPER, G ZILE, MR MCDERMOTT, PJ TI GROWTH EFFECTS OF ELECTRICALLY STIMULATED CONTRACTION ON ADULT FELINE CARDIOCYTES IN PRIMARY CULTURE SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE PROTEIN SYNTHESIS; CELL CULTURE; HYPERTROPHY ID RAT VENTRICULAR MYOCYTES; MOUSE SENSORY NEURONS; CAT RIGHT VENTRICLE; PROTEIN-SYNTHESIS; CARDIAC MYOCYTES; PRESSURE OVERLOAD; GENE-EXPRESSION; LOAD REGULATION; CELL-CULTURE; MUSCLE-CELLS AB The purpose of this study was to determine effects of long-term electrical stimulation of cardiocyte contraction on protein synthesis rates and total protein content. Adult feline cardiocytes were plated on laminin-coated culture trays and maintained in a serum-free medium consisting of M199 supplemented with ascorbate, bovine serum albumin, creatine, carnitine, taurine, and 10(-7) M recombinant insulin. Cardiocytes were electrically stimulated to contract with use of continuous electrical pulses of alternating polarity at a frequency of 1 Hz and pulse duration of 5 ms. Nonstimulated cardiocytes are normally quiescent and were used as the control group. In control quiescent cardiocytes, protein synthesis rate decreased by 14% between days 1 and 4 in culture and then remained stable through day 7. In electrically stimulated cardiocytes, protein synthesis rates increased by 19% between days 1 and 7. Protein synthesis rates were 18% higher on day 4 and 43% higher on day 7 in electrically stimulated than in quiescent cardiocytes. Protein content per cell was determined by measuring total fluorescence per cell by use of confocal microscopy of fluorescein isothiocyanate-stained cells. Electrical stimulation significantly increased cellular protein content by 52% after 7 days compared with controls. Quiescent and electrically stimulated cardiocytes remained rod shaped, retained their myofibrillar architecture, and were responsive to electrical stimulation over the 7-day period. These data demonstrated that electrically stimulated contraction of adult cardiocytes resulted in cell growth, as assessed by an increase in protein content per cell over 7 days in culture. This increase was due, at least in part, to an acceleration of steady-state protein synthesis rates. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, CARDIOL SECT, CHARLESTON, SC 29401 USA. GAZES CARDIAC RES INST, DEPT MED, CHARLESTON, SC 29401 USA. GAZES CARDIAC RES INST, DEPT PHYSIOL, CHARLESTON, SC 29401 USA. GAZES CARDIAC RES INST, DEPT CELL BIOL & ANAT, CHARLESTON, SC 29401 USA. NR 43 TC 41 Z9 41 U1 1 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD JUN PY 1995 VL 268 IS 6 BP H2495 EP H2504 PG 10 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA RE373 UT WOS:A1995RE37300039 ER PT J AU JENDEN, DJ SCREMIN, OU AF JENDEN, DJ SCREMIN, OU TI EFFECTS OF HYPOXIA AND HYPERCAPNIA ON WHOLE-BODY RELEASE AND CLEARANCE OF CHOLINE SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY LA English DT Article DE CHOLINE TURNOVER; CHOLINE CLEARANCE; CHOLINE RELEASE; METABOLIC ACIDOSIS; COMPARTMENT MODEL ID PLASMA CHOLINE; ESSENTIAL NUTRIENT; BRAIN CHOLINE; RAT-BRAIN; ACETYLCHOLINE; TRANSPORT; KINETICS; TURNOVER; EXCHANGE; INVIVO AB We have recently demonstrated an increase in arterial blood choline (Ch) concentration in normocapnic hypoxia and apnea. This could be due to enhanced release of free Ch from tissues, to decreased Ch clearance, or both. The present investigation was undertaken to determine the individual contributions of these processes to the whole body balance of Ch, using an intravenous infusion of tracer quantities of [H-2(4)]Ch to assess the bidirectional flux between the central pool and peripheral pools. Rats were subjected to normocapnic hypoxia or hypercapnia; release and clearance of Ch were calculated using a simple model. Hypoxia caused an increase in Ch production and a decrease in Ch clearance. At severe levels of hypoxia, Ch clearance was essentially zero. Hypoxia was attended by progressive acidosis that was related to the magnitude of the hypoxic challenge. To determine the possible effects of acidosis per se on the variables measured, respiratory acidosis with normoxia was provoked by controlled administration of CO2. Under these conditions, parallel decreases in Ch production and Ch clearance were observed. C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT PHARMACOL, LOS ANGELES, CA 90095 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYS, LOS ANGELES, CA 90095 USA. W LOS ANGELES VET AFFAIRS MED CTR, CTR GERIATR RES EDUC & CLIN, LOS ANGELES, CA 90073 USA. NR 27 TC 5 Z9 5 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6119 J9 AM J PHYSIOL-REG I JI Am. J. Physiol.-Regulat. Integr. Compar. Physiol. PD JUN PY 1995 VL 268 IS 6 BP R1520 EP R1525 PG 6 WC Physiology SC Physiology GA RE357 UT WOS:A1995RE35700024 ER PT J AU ASCH, DA HERSHEY, JC AF ASCH, DA HERSHEY, JC TI WHY SOME HEALTH POLICIES DONT MAKE SENSE AT THE BEDSIDE SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID DECISION-ANALYSIS; INDIVIDUAL PATIENTS; PORTFOLIO THEORY; UTILITY; RISK AB Cost-effectiveness analysis and other forms of decision analysis are becoming more common in the medical literature and are increasingly influential in the development of health policy. Nevertheless, many clinicians find it difficult to apply policies developed from these analyses to individual encounters with patients. We examine the assumptions behind these analyses and argue that the perspective they embody can make clinical strategies appear to be less risky in theory than they are at the bedside. We believe that this problem underlies the intuitive concern many physicians have about policy analyses and calls into question the value of these analyses in shaping clinical practice. These analyses aggregate the benefits and burdens of alternative interventions across different individual persons. Thus, overall population risk appears blunted, as it would in a diversified portfolio of stocks that react differently to financial forces or in a herd of cattle that react differently to veterinary interventions. The assumptions behind these analyses make sense if aggregate outcome is what matters, but not if one cares about each individual investment or animal. Because such aggregation tends to understate individual risk, when applied to human health policy, it may misrepresent the interests of patients and cannot be assumed to provide useful guidelines for decision making at the bedside. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. UNIV PENN,WHARTON SCH,PHILADELPHIA,PA 19104. FU NHGRI NIH HHS [R011HG00621, R011HG00616] NR 26 TC 72 Z9 73 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUN 1 PY 1995 VL 122 IS 11 BP 846 EP 850 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA RA139 UT WOS:A1995RA13900007 PM 7741370 ER PT J AU POLSEN, C ANOUS, M NETSCHER, D SHENAQ, S SAFI, HJ AF POLSEN, C ANOUS, M NETSCHER, D SHENAQ, S SAFI, HJ TI HYPOTHERMIA AND CARDIOPULMONARY BYPASS DURING RESECTION OF EXTENSIVE ARTERIOVENOUS MALFORMATION FOLLOWED BY MICROVASCULAR RECONSTRUCTION SO ANNALS OF PLASTIC SURGERY LA English DT Note ID INTRA-ARTERIAL EMBOLIZATION; CIRCULATORY ARREST; VASCULAR MALFORMATIONS; SURGICAL-TREATMENT; DEEP HYPOTHERMIA; HEMANGIOMAS; ANEURYSM; SURGERY; INFANCY AB A patient is presented in whom hypothermic hypoperfusion and cardiopulmonary bypass were used to aid in resection of a mandibular arteriovenous malformation, This was followed by immediate microvascular reconstruction using a fibular osteocutaneous microvascular free tissue transfer. Prior attempted resection after highly selective embolization had proved inadequate, We found that the operative field, using hypothermic hypoperfusion was bloodless and enabled safe, rapid excision of the malformation, After patient rewarming, microvascular reconstruction was readily performed, No neurologic sequelae resulted, and no recurrence of the malformation has been noted. C1 BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT ANESTHESIA,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT SURG,HOUSTON,TX 77030. DEPT VET AFFAIRS MED CTR,SURG SERV,HOUSTON,TX. NR 47 TC 3 Z9 3 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0148-7043 J9 ANN PLAS SURG JI Ann. Plast. Surg. PD JUN PY 1995 VL 34 IS 6 BP 642 EP 649 DI 10.1097/00000637-199506000-00014 PG 8 WC Surgery SC Surgery GA RC512 UT WOS:A1995RC51200016 PM 7661544 ER PT J AU ALLENDOERFER, R LOEBENBERG, D RINALDI, MG GRAYBILL, JR AF ALLENDOERFER, R LOEBENBERG, D RINALDI, MG GRAYBILL, JR TI EVALUATION OF SCH51048 IN AN EXPERIMENTAL-MODEL OF PULMONARY ASPERGILLOSIS SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID INVASIVE ASPERGILLOSIS AB The efficacy of a novel triazole, SCH51048, was assessed with a murine model of pulmonary aspergillosis and was compared with those of SCH39304 and itraconazole. A wide range of doses of SCH51048 (5 to 50 mg/kg of body weight) was evaluated. Mortality was significantly delayed in mice treated with doses of 5 mg of SCH51048 per kg or greater in comparison with mortality in controls (P < 0.05). Both SCH51048 and SCH39304 at higher doses (30 and 50 mg/kg) reduced the number of viable Aspergillus fumigatus organisms in lung tissue (P < 0.05). In the present model, itraconazole neither delayed mortality nor significantly reduced the counts in tissue at the doses used, We conclude that SCH51048 is an effective therapy for murine pulmonary aspergillosis. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX. AUDIE L MURPHY VET AFFAIRS HOSP,SAN ANTONIO,TX. SCHERING PLOUGH CORP,RES INST,KENILWORTH,NJ. NR 14 TC 17 Z9 18 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD JUN PY 1995 VL 39 IS 6 BP 1345 EP 1348 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RB370 UT WOS:A1995RB37000023 PM 7574528 ER PT J AU WALSH, TJ PETER, J MCGOUGH, DA FOTHERGILL, AW RINALDI, MG PIZZO, PA AF WALSH, TJ PETER, J MCGOUGH, DA FOTHERGILL, AW RINALDI, MG PIZZO, PA TI ACTIVITIES OF AMPHOTERICIN-B AND ANTIFUNGAL AZOLES ALONE AND IN COMBINATION AGAINST PSEUDALLESCHERIA-BOYDII SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Note ID CENTRAL NERVOUS-SYSTEM; PETRIELLIDIUM-BOYDII; BRAIN-ABSCESS; TRANSPLANT RECIPIENT; FUNGAL-INFECTIONS; ENDOCARDITIS; KETOCONAZOLE; KERATITIS AB In order to develop new approaches to treatment of infections due to Pseudallescheria boydii, the in vitro antifungal activity of amphotericin B alone and in combination with miconazole, itraconazole, and fluconazole was studied. Combinations of amphotericin B and antifungal azoles were synergistic, additive, or indifferent in their interaction against P. boydii. Antagonism was not observed. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. RP WALSH, TJ (reprint author), NCI,INFECT DIS SECT,BLDG 10,RM 13N-240,BETHESDA,MD 20892, USA. NR 28 TC 94 Z9 96 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD JUN PY 1995 VL 39 IS 6 BP 1361 EP 1364 PG 4 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA RB370 UT WOS:A1995RB37000026 PM 7574531 ER PT J AU BRANIGAN, PJ GERARD, HC SAAIBI, D HUDSON, AP SCHUMACHER, HR AF BRANIGAN, PJ GERARD, HC SAAIBI, D HUDSON, AP SCHUMACHER, HR TI SCREENING OF SYNOVIAL TISSUE VS FLUID FROM PATIENTS WITH REITERS-SYNDROME (RS), OTHER SPONDYLOARTHROPATHIES, OR REACTIVE ARTHRITIS (REA) FOR CHLAMYDIA VIA POLYMERASE CHAIN-REACTION (PCR) SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV PENN,VET AFFAIRS MED CTR,SCH MED,PHILADELPHIA,PA 19104. MED COLL PENN,PHILADELPHIA,PA 19104. NR 0 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD JUN PY 1995 VL 38 IS 6 SU S BP R20 EP R20 PG 1 WC Rheumatology SC Rheumatology GA RD908 UT WOS:A1995RD90800062 ER PT J AU GERARD, HC BRANIGAN, PJ SCHUMACHER, HR HUDSON, AP AF GERARD, HC BRANIGAN, PJ SCHUMACHER, HR HUDSON, AP TI INAPPARENTLY INFECTING CHLAMYDIA-TRACHOMATIS IN THE SYNOVIA OF REITERS-SYNDROME (RS) REACTIVE ARTHRITIS (REA) PATIENTS ARE VIABLE SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 MED COLL PENN,VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,PHILADELPHIA,PA 19104. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD JUN PY 1995 VL 38 IS 6 SU S BP R24 EP R24 PG 1 WC Rheumatology SC Rheumatology GA RD908 UT WOS:A1995RD90800077 ER PT J AU PARK, J GOWIN, K SCHUMACHER, HR AF PARK, J GOWIN, K SCHUMACHER, HR TI STEROID-SPARING EFFECT OF METHOTREXATE IN RELAPSING POLYCHONDRITIS SO ARTHRITIS AND RHEUMATISM LA English DT Meeting Abstract C1 UNIV PENN,PHILADELPHIA VA MED CTR,PHILADELPHIA,PA 19104. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD JUN PY 1995 VL 38 IS 6 SU S BP R30 EP R30 PG 1 WC Rheumatology SC Rheumatology GA RD908 UT WOS:A1995RD90800112 ER PT J AU BALL, TC HIRAYAMA, F OGAWA, M AF BALL, TC HIRAYAMA, F OGAWA, M TI LYMPHOHEMATOPOIETIC PROGENITORS OF NORMAL MICE SO BLOOD LA English DT Article ID HEMATOPOIETIC STEM-CELLS; DIFFERENTIATION; COLONIES; PROLIFERATION; ORIGIN AB We have identified and characterized the lymphohematopoietic progenitors in the bone marrow of normal mice using a single-step methylcellulose culture assay, Lineage-negative Ly-6A/E (Sca-1)(+) progenitors isolated from normal mice were plated in methylcellulose culture containing steel factor (SF), interleukin-7 (IL-7), erythropoietin (Ep), and IL-11, After 16 to 17 days of culture, pre-B-cell-containing multilineage myeloid colonies can be microscopically identified; however, flow-cytometric analysis of individual colonies for B220-positive cells proved superior to in situ microscopic identification of lymphomyeloid colonies, Approximately 10% (6/66) of the mixed colonies without a conspicuous B-cell component had B22O-positive cells, The single cell origin of the lymphomyeloid colonies was confirmed by micromanipulation, Although the combination of SF, IL-7, and Ep was sufficient to support formation of lymphomyeloid colonies, addition of IL-11, granulocyte colony-stimulating factor or IL-12 to the combination of SF, IL-7, and Ep increased the number of lymphomyeloid colonies, IL-1 alpha and IL-3 independently inhibited the expression of the B-lymphoid lineage when added to the combination of SF, IL-7, Ep, and IL-ll. Approximately four times more lymphohematopoietic progenitors are present in normal mice than in mice treated with 5-fluorouracil. (C) 1995 by The American Society of Hematology. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC 29401. MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. FU NIDDK NIH HHS [DK32294] NR 12 TC 17 Z9 17 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD JUN 1 PY 1995 VL 85 IS 11 BP 3086 EP 3092 PG 7 WC Hematology SC Hematology GA RA136 UT WOS:A1995RA13600010 PM 7756642 ER PT J AU GRELLIER, P YEE, D GONZALEZ, M ABBOUD, SL AF GRELLIER, P YEE, D GONZALEZ, M ABBOUD, SL TI CHARACTERIZATION OF INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS (IGFBP) AND REGULATION OF IGFBP-4 IN BONE-MARROW STROMAL CELLS SO BRITISH JOURNAL OF HAEMATOLOGY LA English DT Article DE INSULIN-LIKE GROWTH FACTOR; BINDING PROTEIN; BONE MARROW; STROMAL CELLS; HEMATOPOIESIS ID MESSENGER-RIBONUCLEIC-ACID; OSTEOBLAST-LIKE CELLS; BREAST-CANCER CELLS; FACTOR-I; HUMAN FIBROBLASTS; RAT; EXPRESSION; LINE; GENE; POTENTIATION AB Bone marrow stromal cells synthesize and secrete insulin-like growth factor (IGF)-I and IGF-binding proteins (IGFBP). IGFBPs may modulate the action of IGF-I or IGF-II on haemopoiesis. However, the specific IGFBPs produced by various stromal cell types have not been identified. We examined six different stromal phenotypes for IGFBP protein and IGFBP-1 to -6 mRNA expression. [I-125]IGF-I ligand blot analysis of conditioned medium demonstrate different patterns of IGFBP secretion by each cell type. The most prominent IGFBPs were 24 and 29 kD species, consistent with IGFBP4 and IGFBP5, respectively, RNase protection assays demonstrate that, overall, stromal cells express IGFBP-2 to -6 mRNAs, with IGFBP4, IGFBP5 and IGFBP6 mRNAs predominating, Since agents that modulate cAMP levels may influence haemopoiesis via the release of stromal-derived cytokines, we determined the effect of forskolin, a cAMP agonist, on IGFBP4 expression in TC-1 cells, Forskolin (10(-5) M) up-regulated IGFBP4 mRNA and protein secretion in a time-dependent manner, These findings suggest that IGFBP-4, -5 and -6 released by stromal cells may be key modulators of the haemopoietic response to IGFs. Release of IGFBP4 by agents that increase cAMP may be an important mechanism involved in regulating IGF bioavailability in the marrow microenvironment. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT ONCOL,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NCI NIH HHS [P30-CA54174, CA52952]; NIAMS NIH HHS [AR42306] NR 36 TC 22 Z9 23 U1 0 U2 1 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0007-1048 J9 BRIT J HAEMATOL JI Br. J. Haematol. PD JUN PY 1995 VL 90 IS 2 BP 249 EP 257 DI 10.1111/j.1365-2141.1995.tb05144.x PG 9 WC Hematology SC Hematology GA RB868 UT WOS:A1995RB86800003 PM 7540852 ER PT J AU NAGAI, H SPIELMAN, AI DASSO, M HUQUE, T BRAND, JG AF NAGAI, H SPIELMAN, AI DASSO, M HUQUE, T BRAND, JG TI RAPID KINETICS OF 2ND MESSENGER PRODUCTION IN BITTER TASTE SO CHEMICAL SENSES LA English DT Meeting Abstract C1 SUNTORY LTD,SUNTORY RES CTR,INST FUNDAMENTAL RES,SHIMAMOTO,OSAKA 618,JAPAN. MONELL CHEM SENSES CTR,PHILADELPHIA,PA 19104. NYU,COLL DENT,NEW YORK,NY. UNIV PENN,VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0379-864X J9 CHEM SENSES JI Chem. Senses PD JUN PY 1995 VL 20 IS 3 BP 358 EP 358 PG 1 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA RE979 UT WOS:A1995RE97900032 ER PT J AU MAREL, M STASTNY, B MELINOVA, L SVANDOVA, E LIGHT, RW AF MAREL, M STASTNY, B MELINOVA, L SVANDOVA, E LIGHT, RW TI DIAGNOSIS OF PLEURAL EFFUSIONS - EXPERIENCE WITH CLINICAL-STUDIES, 1986 TO 1990 SO CHEST LA English DT Article DE CEA; PLEURAL EFFUSION; PLEURAL FLUID; PLEURAL MALIGNANCY ID THORACOSCOPY AB Objectives: To identify in patients with pleural effusion which procedures are most useful in separating malignant from nonmalignant pleural effusions and to identify which procedures most commonly lead to a definitive diagnosis. Design: Prospective consecutive case series. Setting: Pulmonary referral hospital in Prague, Czech Republic. Patients: One hundred seventy-one adults between ages 18 and 70 years with a pleural effusion and a Karnofsky score of 70 or above. Interventions: All patients underwent history, physical, pleural fluid cytologic study, laboratory evaluation of serum and pleural fluid, pleural biopsy, bronchoscopy, and lung scan and/or pulmonary arteriogram. Results: In this series in which 45% of the patients had malignant effusions, 19% had paramalignant effusions, and 36% had benign diseases, the pleural fluid cytologic study was the best for establishing a diagnosis. The pleural fluid carcinoembryonic antigen (CEA) levels above 10 had a high specificity (90%) for malignancy but had low sensitivity (37%). The pleural fluid CEA level was increased only in 19% of patients with paramalignant effusions. Although there were statistically significant differences in the mean results on several biochemical tests of pleural fluid, none were very accurate in separating malignant from benign disease. Conclusion: From this study, we conclude that patients with an undiagnosed pleural effusion should be evaluated in an individualized stepwise manner. If malignancy is strongly considered, the initial three steps should be relatively noninvasive and include clinical evaluation and cytologic study. C1 CHARLES UNIV,PNEUMOL CLIN,PRAGUE,CZECH REPUBLIC. US DEPT VET AFFAIRS,PULM SECT,LONG BEACH,CA. NR 14 TC 76 Z9 80 U1 0 U2 4 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD JUN PY 1995 VL 107 IS 6 BP 1598 EP 1603 DI 10.1378/chest.107.6.1598 PG 6 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA RD451 UT WOS:A1995RD45100024 PM 7781353 ER PT J AU BONORA, E GULLI, G BONADONNA, R DELPRATO, S SOLINI, A DEFRONZO, RA AF BONORA, E GULLI, G BONADONNA, R DELPRATO, S SOLINI, A DEFRONZO, RA TI INSULIN SENSITIVITY IS NOT IMPAIRED IN MEXICAN-AMERICAN WOMEN WITHOUT A FAMILY HISTORY OF DIABETES SO DIABETES CARE LA English DT Article ID ABDOMINAL FAT; GLUCOSE-METABOLISM; BODY-COMPOSITION; PIMA-INDIANS; MELLITUS; OBESITY; MUSCLE; POPULATION; RESISTANCE; NONOBESE AB OBJECTIVE - The purpose of this research was to compare insulin sensitivity in Mexican-Americans and non-Hispanic whites without a family history of diabetes to establish whether insulin resistance is a defect intrinsically related to subjects of Mexican origin. RESEARCH DESIGN AND METHODS - In study A, we compared insulin sensitivity in 12 Mexican-American and 12 non-Hispanic white women with normal glucose tolerance and no family history of diabetes. In study B, we compared insulin sensitivity in two groups of normal glucose-tolerant Mexican-Americans, nine with a positive (FHD+) and nine with a negative (FHD-) family history of diabetes. In both studies, the groups were closely matched for age, total body fat content, and fat topography. Insulin sensitivity was assessed with the euglycemic insulin clamp (20 mU . min(-1) . m(2) surface area) which was performed in combination with tritiated glucose infusion and indirect calorimetry. Total fat mass and fat-free mass (FFM) were assessed by a tritiated water dilution technique, and regional fat distribution was evaluated by anthropometry and magnetic resonance imaging. RESULTS - During a 4-h euglycemic insulin clamp (study A), rates (mg . min(-1) . kg FFM(-1)) of total (6.32 +/- 0.64 vs. 6.62 +/- 0.81), oxidative (3.54 +/- 0.24 vs. 3.51 +/- 0.19), and nonoxidative (2.78 +/- 0.48 vs. 3.11 +/- 0.75) glucose utilization were similar in Mexican-Americans and non-Hispanic whites; hepatic glucose production (0.33 +/- 0.13 vs. 0.35 +/- 0.13) was suppressed similarly in both groups. During a 2-h euglycemic insulin clamp (study B), Mexican-Americans with FHD+ had lower rates of insulin-mediated total (3.55 +/- 0.39 vs. 5.93 +/- 0.59, P < 0.001), oxidative (3.31 +/- 0.25 vs. 4.32 +/- 0.17, P < 0.01), and nonoxidative (0.24 +/- 0.28 vs. 1.61 +/- 0.49, P < 0.01) glucose disposal than subjects with FHD-; suppression of hepatic glucose production (0.24 +/- 0.14 vs. 0.18 +/- 0.12) was similar in both groups. CONCLUSIONS - These results indicate that in the absence of a family history of noninsulin-dependent diabetes mellitus, Mexican-American women are not less sensitive to insulin than non-Hispanic white women. C1 UNIV TEXAS,HLTH SCI CTR,DEPT INTERNAL MED,DIV DIABET,SAN ANTONIO,TX. AUDIE L MURPHY VET AFFAIRS HOSP,SAN ANTONIO,TX. RI Del Prato, Stefano/K-3405-2016; Solini, Anna/K-4666-2016 OI Del Prato, Stefano/0000-0002-5388-0270; Solini, Anna/0000-0002-7855-8253; BONORA, Enzo/0000-0003-1074-5164 FU NCRR NIH HHS [M01-RR-01346]; NIADDK NIH HHS [AM-24092] NR 38 TC 6 Z9 6 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0149-5992 J9 DIABETES CARE JI Diabetes Care PD JUN PY 1995 VL 18 IS 6 BP 825 EP 833 DI 10.2337/diacare.18.6.825 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RB510 UT WOS:A1995RB51000012 PM 7555509 ER PT J AU CUSI, K CUNNINGHAM, GR COMSTOCK, JP AF CUSI, K CUNNINGHAM, GR COMSTOCK, JP TI SAFETY AND EFFICACY OF NORMALIZING FASTING GLUCOSE WITH BEDTIME NPH INSULIN ALONE IN NIDDM SO DIABETES CARE LA English DT Article ID DEPENDENT DIABETES-MELLITUS; SECONDARY FAILURE; DAYTIME SULFONYLUREA; THERAPY; SECRETION; OBESE; TRIAL; RESISTANCE; 1ST-PHASE; DISEASE AB OBJECTIVE - To examine the safety and overall clinical effects of normalizing the fasting plasma glucose (FPG) level with bedtime NPH insulin alone in patients with non-insulin-dependent diabetes mellitus (NIDDM) that is poorly controlled with maximal doses of sulfonylureas. RESEARCH DESIGN AND METHODS - Twelve obese male NIDDM subjects were treated for 16 weeks with bedtime insulin after a 4-week sulfonylurea washout. The insulin dosage was increased until the FPG level was normalized. The 24-h plasma glucose profiles and lipid and HbA(1c) levels were measured at the beginning and end of the study, and the incidence and severity of hypoglycemic episodes were closely monitored. In addition, hyperglycemic clamp studies were performed to assess insulin secretion and provide an indirect measurement of insulin sensitivity. RESULTS - FPG (14.6 +/- 0.9 mmol/l at week 0) was normalized (<6.4 mmol/l) within 6 weeks (5.9 +/- 0.6 mmol/l) and remained at target levels until the end of the study (4.0 +/- 0.03 mmol/l at week 16, P < 0.001). The insulin dose was 80 +/- 9 U/day (0.86 +/- 0.10 U/kg). Improved glycemic control was confirmed by a reduction in HbA(1c)(10.9 +/- 0.05 vs. 7.2 +/- 0.2%, P < 0.001) and mean 24-h glucose (17.2 +/- 0.2 vs. 7.4 +/- 0.2 mmol/l, P < 0.001). The incidence of mild or moderate hypoglycemic episodes was 3.4 +/- l/patient for the entire 16-week study, and no patient experienced severe hypoglycemia. Bedtime insulin significantly improved total cholesterol, low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, and triglyceride levels (P < 0.01). Weight gain was 2.4 +/- 0.7 kg, and blood pressure was unchanged. During the hyperglycemic clamp, there was an improvement in the first phase (P < 0.001) and in the second phase (P < 0.01) of insulin secretion. There also was an increase in the rate of exogenous glucose infused (M) (P < 0.01) and in the M/C-peptide ratio (P < 0.02), suggesting enhanced insulin sensitivity. CONCLUSIONS - NPH insulin given at bedtime in amounts sufficient to achieve a normal FPG level does not cause excessive or severe hypoglycemia and does lead to good glycemic and lipid control in NIDDM. Bedtime insulin therapy also is accompanied by improved insulin secretion and insulin sensitivity. We conclude that a single dose of insulin alone at bedtime merits consideration as a therapeutic strategy in patients with poorly controlled NIDDM. C1 METHODIST HOSP,GEN CLIN RES CTR,HOUSTON,TX. DEPT VET AFFAIRS MED CTR,ENDOCRINOL SECT,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. FU NCRR NIH HHS [MO1-RR-00350-25] NR 49 TC 57 Z9 57 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0149-5992 J9 DIABETES CARE JI Diabetes Care PD JUN PY 1995 VL 18 IS 6 BP 843 EP 851 DI 10.2337/diacare.18.6.843 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RB510 UT WOS:A1995RB51000014 PM 7555511 ER PT J AU GIORDANO, M CASTELLINO, P CARROLL, CA DEFRONZO, RA AF GIORDANO, M CASTELLINO, P CARROLL, CA DEFRONZO, RA TI COMPARISON OF THE EFFECTS OF HUMAN RECOMBINANT INSULIN-LIKE GROWTH-FACTOR-I AND INSULIN ON PLASMA AMINO-ACID-CONCENTRATIONS AND LEUCINE KINETICS IN HUMANS SO DIABETOLOGIA LA English DT Article DE LEUCINE; KETOISOCAPROATE; INSULIN; IGF-I; PROTEIN METABOLISM ID IGF-I; RENAL-FUNCTION; GLUCOSE; METABOLISM; SECRETION; RECEPTORS; INFUSION; BINDING AB We examined the effects of recombinant human insulin-like growth factor I (IGF-I) and insulin on the plasma amino acid (AA) profile and leucine kinetics in eight normal subjects. IGF-I was infused at 52 pmol . kg(-1). min(-1) in combination with prime-continuous [1-C-14] leucine infusion, to obtain steady-state plasma concentrations of total (54 +/- 3 nmol/l) and free (7.3 +/- 1 nmol/l) IGF-I (study 1). In response to IGF-I, plasma AA levels declined by 37 +/- 3% (1975 +/- 198 to 1368 +/- 120 mu mol/l) and total branched chain amino acids (BCAA) declined by 34 +/- 3% (390 +/- 21 to 256 +/- 13 mu mol/l). This hypoaminoacidaemic effect was associated with a decline in endogenous leucine flux of 17 +/- 2% (1.88 +/- 0.05 to 1.57 +/- 0.04 mu mol . kg(-1). min(-1)) and leucine oxidation of 17 +/- 1% (0.31 +/- 0.02 vs 0.26 +/- 0.02 mu mol . kg(-1). min(-1)) (both p < 0.01 vs basal), The same subjects underwent a second study (study 2) in which insulin was infused at 6.22 pmol . kg(-1). min(-1) to obtain a steady-state plasma insulin concentration of 530 +/- 25 pmol/l while maintaining euglycaemia. The infusion rate was designed to match the declines in plasma BCAA levels and leucine turnover observed during IGF-I infusion. The rates of glucose infusion necessary to maintain euglycaemia during IGF-I and insulin infusion were 4.9 +/- 1.0 and 7.8 +/- 0.6 mg . kg(-1). min(-1), respectively. During insulin infusion total BCAA declined by 39% from 369 +/- 23 to 226 +/- 20 mu mol/l, leucine flux declined by 16 +/- 2% from 1.90 +/- 0.05 to 1.61 +/- 0.03 mu mol . kg(-1). min(-1), and leucine oxidation declined by 19 +/- 2% from 0.32 +/- 0.02 to 0.26 +/- 0.02 mu mol . kg(-1). min(-1). On a molar basis IGF-I was 7.3% as potent as insulin in inhibiting proteolysis. These results demonstrate that in humans: (i) the hypoaminoacidaemic response to IGF-I can be entirely ascribed to the inhibition of proteolysis; (ii) qualitatively, the effects of IGF-I and insulin on plasma AA profile and protein metabolism are similar; (iii) quantitatively, IGF-I is 14-fold less potent than insulin in suppressing protein degradation. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV DIABET,SAN ANTONIO,TX 78284. UNIV NAPLES 2,INST INTERNAL MED & NEPHROL,NAPLES,ITALY. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. OI CASTELLINO, Pietro/0000-0002-9014-2007 FU NCRR NIH HHS [M01-RR-01346] NR 26 TC 8 Z9 8 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0012-186X J9 DIABETOLOGIA JI Diabetologia PD JUN PY 1995 VL 38 IS 6 BP 732 EP 738 DI 10.1007/BF00401848 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QZ605 UT WOS:A1995QZ60500017 PM 7672498 ER PT J AU MONTOYA, ID LEVIN, FR FUDALA, PJ GORELICK, DA AF MONTOYA, ID LEVIN, FR FUDALA, PJ GORELICK, DA TI DOUBLE-BLIND COMPARISON OF CARBAMAZEPINE AND PLACEBO FOR TREATMENT OF COCAINE DEPENDENCE SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE COCAINE; DEPENDENCE; CARBAMAZEPINE ID USERS; LIDOCAINE; SEIZURES; DESIGN; DRUGS; RATS AB This study was conducted to determine the effectiveness of carbamazepine (CBZ) for treatment of cocaine dependence. Sixty-two (CBZ = 28, placebo = 34) cocaine-dependent (DSM-III-R criteria) volunteers consented to be treated for eight weeks with standardized outpatient individual counseling twice a week plus double-blind CBZ or inactive placebo. During the 8-week trial, both groups showed increased number of urine samples negative for cocaine, significantly (P < 0.01) decreased self-reported cocaine use (money spent and grams used), and decreased Beck Depression Inventory and Symptom Check List-90-Revised (SCL-90-R) total scores. However, there were no significant differences between CBZ and placebo. This study does not support the effectiveness of CBZ for outpatient treatment of cocaine dependence. C1 NIDA,INTRAMURAL RES PROGRAM,TREATMENT BRANCH,BALTIMORE,MD 21224. COLUMBIA UNIV,DEPT PSYCHIAT,NEW YORK,NY. UNIV PENN,DEPT PSYCHIAT,PHILADELPHIA,PA 19104. DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. UNIV MARYLAND,DEPT PSYCHIAT,BALTIMORE,MD. FU Intramural NIH HHS [Z99 DA999999] NR 37 TC 44 Z9 44 U1 1 U2 1 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD JUN PY 1995 VL 38 IS 3 BP 213 EP 219 DI 10.1016/0376-8716(95)01101-4 PG 7 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA RF947 UT WOS:A1995RF94700003 PM 7555621 ER PT J AU CORNISH, JW MAANY, I FUDALA, PJ NEAL, S POOLE, SA VOLPICELLI, P OBRIEN, CP AF CORNISH, JW MAANY, I FUDALA, PJ NEAL, S POOLE, SA VOLPICELLI, P OBRIEN, CP TI CARBAMAZEPINE TREATMENT FOR COCAINE DEPENDENCE SO DRUG AND ALCOHOL DEPENDENCE LA English DT Article DE CARBAMAZEPINE; COCAINE DEPENDENCE; PHARMACOTHERAPY; URINARY BENZOYLECGONINE; CRAVING ID METHADONE-MAINTAINED PATIENTS; DESIPRAMINE; AMANTADINE; DETOXIFICATION; BROMOCRIPTINE; DOPAMINE AB We report on a double-blind, placebo-controlled study of carbamazepine (CBZ) treatment for cocaine dependence. A previously reported uncontrolled study found CBZ to be a beneficial pharmacotherapy for cocaine dependence. Statistical analyses were performed on data from 82 subjects who were randomized to 10 weeks' treatment with either CBZ, titrated to 4-12 mu g/ml, (n = 37) or placebo (n = 45). The two treatment groups did not differ for primary outcome measures of retention time in treatment, urine samples positive for cocaine metabolite, subject reported desire for cocaine or for subject reported side-effects. CBZ was not an effective treatment in this study. C1 DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RUTGERS STATE UNIV,DEPT PSYCHOL,NEW BRUNSWICK,NJ 08903. RP CORNISH, JW (reprint author), UNIV PENN,PHILADELPHIA VA MED CTR,DEPT PSYCHIAT,3900 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. FU NIDA NIH HHS [DA 00144, DA 05186] NR 26 TC 40 Z9 40 U1 3 U2 3 PU ELSEVIER SCI PUBL IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0376-8716 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD JUN PY 1995 VL 38 IS 3 BP 221 EP 227 DI 10.1016/0376-8716(95)01102-5 PG 7 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA RF947 UT WOS:A1995RF94700004 PM 7555622 ER PT J AU WICH, BK CARNES, M AF WICH, BK CARNES, M TI MENOPAUSE AND THE AGING FEMALE REPRODUCTIVE-SYSTEM SO ENDOCRINOLOGY AND METABOLISM CLINICS OF NORTH AMERICA LA English DT Review ID ESTROGEN REPLACEMENT THERAPY; POSTMENOPAUSAL WOMEN; PROGESTERONE RECEPTORS; MENSTRUAL-CYCLE; HORMONE-THERAPY; ELDERLY WOMEN; PREVALENCE; MANAGEMENT; ESTRADIOL; SYMPTOMS AB Menopause is a fact of life for women, who live the last third of their lives in a postmenopausal state. Although estrogen replacement therapy offers primary prevention and treatment of vasomotor, urogenital, and osteoporotic symptoms of estrogen deprivation and seems to have beneficial effects on cardiovascular mortality, only 20% of women in the United States currently are taking hormone supplements. This article discusses the menopause-influencing changes in the hypothalamic-pituitary-ovarian axis with associated neuroendocrine and sex hormone changes, their physiologic consequences on the reproductive system, and current recommendations for hormone replacement therapy. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON GERIATR RES EDUC & CLIN CTR,MADISON,WI 53705. UNIV WISCONSIN,DEPT MED,MADISON,WI. NR 101 TC 25 Z9 25 U1 0 U2 5 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0889-8529 J9 ENDOCRIN METAB CLIN JI Endocrinol. Metabol. Clin. North Amer. PD JUN PY 1995 VL 24 IS 2 BP 273 EP 295 PG 23 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RD631 UT WOS:A1995RD63100005 PM 7656892 ER PT J AU GINN, GO AF GINN, GO TI BUSINESS STRATEGY AND FINANCIAL STRUCTURE - AN EMPIRICAL-ANALYSIS OF ACUTE-CARE HOSPITALS SO HOSPITAL & HEALTH SERVICES ADMINISTRATION LA English DT Article ID CAPITAL STRUCTURE; MILES; PERFORMANCE AB This study investigated the relationship between business strategy and financial structure in the U.S. hospital industry. We studied two dimensions of financial structure-liquidity and leverage. Liquidity was assessed by the acid ratio, and leverage was assessed using the equity funding ratio. Drawing from managerial, finance, and resource dependence perspectives, we developed and tested hypotheses about the relationship between Miles and Snow strategy types and financial structure. Relevant contextual financial and organizational variables were controlled for statistically through the Multivariate Analysis of Covariance technique. The relationship between business strategy and financial structure was found to be significant. Among the Miles and Snow strategy types, defenders were found to have relatively high liquidity and low leverage. Prospectors typically had low liquidity and high leverage. Implications for financial planning, competitive assessment, and reimbursement policy are discussed. C1 US DEPT VET AFFAIRS,BOSTON,MA. BOSTON UNIV,SCH PUBL HLTH,BOSTON,MA 02215. UNIV NEVADA,DEPT MANAGERIAL SCI,RENO,NV 89557. RP GINN, GO (reprint author), CLARKSON COLL,101 S 42ND ST,OMAHA,NE 68131, USA. NR 33 TC 4 Z9 4 U1 1 U2 8 PU AMER COLL HEALTHCARE EXEC HEALTH ADMINISTRATION PRESS PI CHICAGO PA ONE NORTH FRANKLIN ST SUITE 1700, CHICAGO, IL 60606 SN 8750-3735 J9 HOSP HEALTH SERV ADM JI Hosp. Health Serv. Adm. PD SUM PY 1995 VL 40 IS 2 BP 191 EP 209 PG 19 WC Health Policy & Services SC Health Care Sciences & Services GA QZ638 UT WOS:A1995QZ63800002 PM 10143031 ER PT J AU CARLSON, HE GRABER, ML GELATO, MC HERSHMAN, JM AF CARLSON, HE GRABER, ML GELATO, MC HERSHMAN, JM TI ENDOCRINE EFFECTS OF ERYTHROPOIETIN SO INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS LA English DT Article DE ERYTHROPOIETIN; HORMONES; HEMODIALYSIS ID RECOMBINANT-HUMAN-ERYTHROPOIETIN; IMPROVED SEXUAL FUNCTION; HEMODIALYSIS-PATIENTS; THERAPY; TESTOSTERONE; PROLACTIN; RHUEPO AB Uremic men may manifest a variety of hormonal abnormalities, including decreased serum concentrations of testosterone and thyroid hormones and increased serum levels of growth hormone and prolactin. Some previous investigations have reported that erythropoietin therapy may reverse these hormonal changes. To investigate this possibility further, we measured serum prolactin, testosterone, LH, FSH, TSH, free thyroxine, triiodothyronine, growth hormone and IGF-I in 21 generally elderly male hemodialysis patients before and during erythropoietin therapy; many of the patients also received an anabolic steroid or metoclopramide treatment. Despite a significant erythropoietic response in a majority of the subjects, no significant changes were seen in any of the hormonal parameters other than a small decrease in serum growth hormone concentrations. Advanced age and chronic illness in our patients may have played a role in limiting the hormonal response reported by others. C1 NORTHPORT VET ADM MED CTR,RES SERV,NORTHPORT,NY 11768. SUNY STONY BROOK,DEPT MED,STONY BROOK,NY 11794. W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,RES SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. RP CARLSON, HE (reprint author), NORTHPORT VET ADM MED CTR,MED SERV 111,NORTHPORT,NY 11768, USA. NR 18 TC 3 Z9 6 U1 0 U2 0 PU WICHTIG EDITORE PI MILAN PA 72/74 VIA FRIULI, 20135 MILAN, ITALY SN 0391-3988 J9 INT J ARTIF ORGANS JI Int. J. Artif. Organs PD JUN PY 1995 VL 18 IS 6 BP 309 EP 314 PG 6 WC Engineering, Biomedical; Transplantation SC Engineering; Transplantation GA RY122 UT WOS:A1995RY12200003 PM 8593965 ER PT J AU CRAIG, WA AF CRAIG, WA TI ONCE-DAILY VERSUS MULTIPLE-DAILY DOSING OF AMINOGLYCOSIDES SO JOURNAL OF CHEMOTHERAPY LA English DT Article; Proceedings Paper CT Meeting on Isepamicin Once a Day - Enhancing Traditional Aminoglycoside Therapy CY SEP 24-25, 1994 CL ROTHERWICK, ENGLAND DE ISEPAMICIN; AMINOGLYCOSIDES; ONCE-DAILY ADMINISTRATION AB The pharmacodynamic characteristics of isepamicin and other aminoglycosides, both in terms of efficacy and toxicity, explain why once-daily administration of these agents should be the optimal dosing regimen. Isepamicin, as with other aminoglycosides, exhibits concentration-dependent bactericidal activity and produces prolonged post-antibiotic effects against susceptible organisms. High concentrations of these drugs would be expected to produce more rapid and extensive bacterial killing than lower levels. Furthermore, the post-antibiotic effect would protect against bacterial regrowth when serum and tissue concentrations fall below inhibitory levels. In animal models, the magnitude of the peak serum concentration or the area under the concentration-time curve, are the important determinants of efficacy for isepamicin and the other aminoglycosides. Isepamicin also exhibits the ''first-exposure effect'', i.e. initial exposure of bacteria to isepamicin down-regulates subsequent uptake of the drug. During this period of down-regulation, bacteria exhibit decreased killing and shorter post-antibiotic effects. Since the first-exposure effect lasts for several hours, once-daily administration of the aminoglycosides allows for this effect to dissipate completely between doses. High peak concentrations, greater than 8-10 times the minimum inhibitory concentration (MIG), will also decrease the emergence of resistant strains. With regard to toxicity, one of the first steps in the uptake of aminoglycosides into sites of toxicity is their binding to the brush borders of renal cells and to the cochlea and vestibular membranes. Binding to these membranes demonstrates saturable kinetics. As a result, uptake of the aminoglycosides is more efficient with low sustained concentrations than with high intermittent levels. Once-daily dosing of aminoglycosides has consistently been less toxic than more frequent dosing in animals. In clinical studies, once-daily dosing of aminoglycosides compared to two- or three-times daily administration has generally exhibited similar efficacy and toxicity. However, a few studies have shown greater efficacy or lower toxicity with once-daily dosing of aminoglycosides. Once-daily dosing of the aminoglycosides has the potential to enhance efficacy, reduce toxicity, and lower administration costs for this drug class. RP CRAIG, WA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705, USA. NR 0 TC 43 Z9 44 U1 0 U2 2 PU E I F T SRL PI FLORENCE PA VIA XX SETTEMBRE 102, 50129 FLORENCE, ITALY SN 1120-009X J9 J CHEMOTHERAPY JI J. Chemother. PD JUN PY 1995 VL 7 SU 2 BP 47 EP 52 PG 6 WC Oncology; Infectious Diseases; Pathology; Pharmacology & Pharmacy SC Oncology; Infectious Diseases; Pathology; Pharmacology & Pharmacy GA TG379 UT WOS:A1995TG37900006 PM 8622110 ER PT J AU DELAMATA, J UY, HL GUISE, TA STORY, B BOYCE, BF MUNDY, GR ROODMAN, GD AF DELAMATA, J UY, HL GUISE, TA STORY, B BOYCE, BF MUNDY, GR ROODMAN, GD TI INTERLEUKIN-6 ENHANCES HYPERCALCEMIA AND BONE-RESORPTION MEDIATED BY PARATHYROID HORMONE-RELATED PROTEIN IN-VIVO SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE OSTEOCLAST; MALIGNANCY; CYTOKINE; PARATHYROID HORMONE-RELATED PROTEIN; INTERLEUKIN-6 ID CELL-LINES; NUDE-MICE; IL-6; CARCINOMA; CACHEXIA; MURINE; SERUM AB Tumors frequently induce the multifunctional cytokine IL-6, which has been linked to several paraneoplastic syndromes, most notably cachexia. IL-6 stimulates osteoclast formation, causes mild hypercalcemia, and is produced by bone cells in vitro upon exposure to systemic hormones. Since IL-6 is produced together with parathyroid hormone-related protein (PTH-rP) in some patients with cancer, we tested the hypothesis that production of IL-6 potentiates the effects of PTH-rP on Ca2+ homeostasis and osteoclastic bone resorption and examined potential mechanisms for these interactions in vivo. Chinese hamster ovarian (CHO) cells stably transfected with cDNAs for IL-6 (CHO/IL-6) and PTH-rP sense (CIIO/PTH-rP) or antisense (CHO/PTH-rP AS) were inoculated intramuscularly into nude mice. Experimental groups included CHO/IL-6 plus CHO/PTH-rP; CHO/IL-6 plus CHO/PTH-rP AS; CHO/IL-6 alone; and CHO/PTH-rP alone. Blood ionized Ca2+ was measured on days 0, 7, 10, 12, and 13. Three different developmental stages in the osteoclast lineage were examined at day 13: the early multipotential precursor, granulocyte macrophage colony-forming units (CFU-GM); more mature mononuclear osteoclast precursors, assessed by their capacity to form tartrate-resistant acid phosphatase-positive multinucleated cells in marrow cultures; and mature osteoclasts, assessed by histomorphometry, IL-6 increased CFU-GM but not bone resorption or Ca2+. In contrast, PTH-rP induced hypercalcemia and bone resorption and increased multinucleated osteoclasts and more mature precursors cells; but not CFU-GM. However, mice treated with both IL-6 and PTH-rP had very marked hypercalcemia and osteoclastosis as well as an increase in the number of both CFU-GM: and mature osteoclast precursors. These data demonstrate that IL-6 enhances PTH-rP-mediated hypercalcemia and bone resorption, most likely by increasing the pool of early osteoclast precursors that in turn can differentiate to mature osteoclasts. We conclude that IL-6 stimulatory effects on osteoclast precursors may enhance the effects of other bone resorption factors that act at later stages in the osteoclast lineage. C1 AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV 151,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. FU NIAMS NIH HHS [K08-AR01899, P01-AR39529]; NIDCR NIH HHS [DE08569] NR 21 TC 170 Z9 173 U1 1 U2 3 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JUN PY 1995 VL 95 IS 6 BP 2846 EP 2852 DI 10.1172/JCI117990 PG 7 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA RB212 UT WOS:A1995RB21200053 PM 7769125 ER PT J AU GERACI, JM ASHTON, CM KUYKENDALL, DH JOHNSON, ML WU, L AF GERACI, JM ASHTON, CM KUYKENDALL, DH JOHNSON, ML WU, L TI IN-HOSPITAL COMPLICATIONS AMONG SURVIVORS OF ADMISSION FOR CONGESTIVE-HEART-FAILURE, CHRONIC OBSTRUCTIVE PULMONARY-DISEASE, OR DIABETES-MELLITUS SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Article DE COMPLICATIONS; CONGESTIVE HEART FAILURE; DIABETES MELLITUS; CHRONIC OBSTRUCTIVE PULMONARY DISEASE; IATROGENIC DISEASE AB OBJECTIVE: To determine the frequency of hospital complications among survivors of inpatient treatment for congestive heart failure (CHF), chronic obstructive pulmonary disease (COPD), or diabetes mellitus (DM). DESIGN: Retrospective cohort study, SETTING: Nine Veterans Affairs hospitals in the southern United States. PATIENTS: 1,837 men veterans discharged alive following hospitalization for CHF, COPD, or DM between January 1987 and December 1989. This patient population represents a subset of cases gathered to study the process of care in the hospital and subsequent early readmission; thus, veterans who died in the hospital were not included. MEASUREMENTS: Medical record review to record the occurrence of any of 30 in-hospital complications such as cardiac arrest, nosocomial infections, or delirium (overall agreement between two reviewers = 84%,kappa = 0.37), RESULTS: Complications occurred in 15.7% of the CHF cases, 13.1% of the COPD cases, and 14.8% of the DM cases, Hypoglycemic reactions were the most frequent individual adverse events in the CHF and DM cases (3.6% and 11.4% of the cases, respectively), and theophylline toxicity was most frequent among the COPD cases (4.9%). Patient age, the presence of comorbid diseases, and the Acute Physiology Score (APS) of APACHE II were associated with complication occurrence, For each disease, the patients who had a complication had significantly longer mean hospital stays than did the patients who did not have complications (14.6 to 14.9 days vs 7.2 to 8.2 days, p < 0.01), CONCLUSIONS: Complications are frequent among patients discharged alive with CHF, COPD, or DM, The patients who experienced complications were more ill on admission and had longer hospital stays. RP GERACI, JM (reprint author), BAYLOR COLL MED,HOUSTON VAMC,HOUSTON CTR QUAL CARE & UTILIZAT STUDIES,MED SERV IIIC,HOUSTON,TX 77030, USA. NR 0 TC 17 Z9 17 U1 0 U2 1 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD JUN PY 1995 VL 10 IS 6 BP 307 EP 314 DI 10.1007/BF02599949 PG 8 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA RD788 UT WOS:A1995RD78800002 PM 7562121 ER PT J AU WAZNAWESLY, JM MERANDA, DL CAREY, P SHENKER, Y AF WAZNAWESLY, JM MERANDA, DL CAREY, P SHENKER, Y TI EFFECT OF ATRIAL NATRIURETIC HORMONE ON VASOPRESSIN AND THIRST RESPONSE TO OSMOTIC STIMULATION IN HUMAN-SUBJECTS SO JOURNAL OF LABORATORY AND CLINICAL MEDICINE LA English DT Article ID FACTOR INHIBITS DEHYDRATION; RAT POSTERIOR PITUITARY; WATER-INTAKE; HEALTHY-VOLUNTEERS; PEPTIDE; RELEASE; POLYPEPTIDE; SECRETION; OSMOLALITY; INFUSION AB To evaluate the effect of systemically administered atrial natriuretic hormone (ANH) on osmotically induced secretion of arginine vasopressin (AVP) and thirst sensation, 11 healthy men, aged 18 to 28 years, were studied on four occasions. The intravenous infusions of placebo (P) or one of three doses of ser-tyr(28) human ANH (0.6 [LD], 1.8 [MD], and 5.4 [HD] pmol/kg/min) were given in random order over 2 hours. During the second hour, subjects also received a 5% saline (HS) infusion (0.1 ml/kg/min). The baseline parameters were similar on each of the study days. Plasma ANH levels increased approximately twofold, eightfold, and 25-fold during LD, MD, and HD infusions, respectively. HS infusion caused increases in serum sodium level (5 to 7 mEq/L) and osmolality (14 to 15 mOsm/L) (p < 0.001). During HS infusion on P day, ANH levels almost doubled (p < 0.001). AVP levels remained stable during the first hour of ANH infusions. An addition of HS caused a significant increase in AVP levels (p < 0.001). The magnitude of this increase was similar on each of the study days. Similarly, thirst perception increased significantly (p < 0.01) and to the same extent during HS infusion on all study days. Both AVP levels and thirst showed a very good correlation with serum osmolality on each of the study days, and there were no significant differences between any of the slopes or intercepts. We conclude that short-term elevation of plasma ANH levels up to 25-fold affects neither the osmotically stimulated secretion of AVP nor thirst perception. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MED SERV,ENDOCRINE SECT,MADISON,WI 53705. UNIV WISCONSIN,MADISON,WI. FU NCRR NIH HHS [RR-03186]; NIDDK NIH HHS [1-R29-DK-38444] NR 50 TC 6 Z9 6 U1 0 U2 1 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 0022-2143 J9 J LAB CLIN MED JI J. Lab. Clin. Med. PD JUN PY 1995 VL 125 IS 6 BP 734 EP 742 PG 9 WC Medical Laboratory Technology; Medicine, General & Internal; Medicine, Research & Experimental SC Medical Laboratory Technology; General & Internal Medicine; Research & Experimental Medicine GA RB445 UT WOS:A1995RB44500012 PM 7769367 ER PT J AU SHEKELLE, PG HURWITZ, EL COULTER, I ADAMS, AH GENOVESE, B BROOK, RH AF SHEKELLE, PG HURWITZ, EL COULTER, I ADAMS, AH GENOVESE, B BROOK, RH TI THE APPROPRIATENESS OF CHIROPRACTIC SPINAL MANIPULATION FOR LOW-BACK-PAIN - A PILOT-STUDY SO JOURNAL OF MANIPULATIVE AND PHYSIOLOGICAL THERAPEUTICS LA English DT Article DE CHIROPRACTIC MANIPULATION; BACK PAIN; QUALITY OF CARE AB Objective: Spinal manipulation is an efficacious therapy for some patients with low back pain (LBP). In this pilot study, we tested the feasibility of assessing the appropriateness of chiropractic spinal manipulation for patients with LBP. Methods: Criteria for the appropriate and inappropriate use of spinal manipulation for low back pain were developed using the RAND/UCLA appropriateness method. Two separate expert panels, one multidisciplinary and one all chiropractic, each rated a comprehensive array of clinical scenarios for appropriateness. A random sample of practicing chiropractors was selected, and data were collected from ten randomly selected office records from each participating clinician. Assessment of the appropriateness for the use of spinal manipulation was made by comparing the care delivered with the appropriateness criteria determined by each expert panel. Results: Eight of thirteen (62%) eligible chiropractors agreed to participate. For the remainder, by the multidisciplinary panel's criteria, 38% of care was appropriate and 26% of care was inappropriate. By the all-chiropractic panel's criteria, the same cases were judged 74% appropriate and 7% inappropriate. The two panel's appropriateness ratings were in agreement on 48% of all cases. Conclusions: In this geographic area, the rate of appropriate care is between 38% and 74% and the rate of inappropriate care is between 7% and 19%, depending on the criteria used to assess appropriateness. Data from other geographic areas of the U.S. will be needed before inferences to a larger population may be drawn, and we have demonstrated that such a study is feasible. C1 LOS ANGELES COLL CHIROPRACT,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP SHEKELLE, PG (reprint author), RAND CORP,HLTH SCI PROGRAM,1700 MAIN ST,POB 2138,SANTA MONICA,CA 90407, USA. NR 0 TC 9 Z9 9 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0161-4754 J9 J MANIP PHYSIOL THER JI J. Manip. Physiol. Ther. PD JUN PY 1995 VL 18 IS 5 BP 265 EP 270 PG 6 WC Health Care Sciences & Services; Integrative & Complementary Medicine; Rehabilitation SC Health Care Sciences & Services; Integrative & Complementary Medicine; Rehabilitation GA RD335 UT WOS:A1995RD33500001 PM 7673792 ER PT J AU MORTEL, KF MEYER, JS AF MORTEL, KF MEYER, JS TI LACK OF POSTMENOPAUSAL ESTROGEN REPLACEMENT THERAPY AND THE RISK OF DEMENTIA SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID ISCHEMIC VASCULAR DEMENTIA; ADRDA WORK GROUP; ALZHEIMERS-DISEASE; CARDIOVASCULAR-DISEASE; CLINICAL-DIAGNOSIS; BASAL FOREBRAIN; GROWTH-FACTOR; FOLLOW-UP; WOMEN; STROKE AB Estrogen replacement therapy (EXT) and associated risks for ischemic vascular dementia (IVD) and dementia of the Alzheimer's type (DAT) among postmenopausal women were investigated by determining whether EXT was differently distributed among control subjects than among subjects with dementia. Subjects included 93 with probable DAT, 65 with probable IVD, and 148 normal control subjects. The proportion of control subjects on EXT was almost 2:1, and this ratio holds for both dementia groups. Logistic regression suggests lack of EXT is associated with increased risk for dement ia among elderly women. EXT may eventually prove to be a useful prophylactic agent for reducing risk of DAT and IVD among postmenopausal women. C1 DEPT VET AFFAIRS MED CTR,CEREBRAL BLOOD FLOW LAB,HOUSTON,TX. BAYLOR COLL MED,DEPT NEUROL,HOUSTON,TX 77030. NR 33 TC 99 Z9 101 U1 1 U2 3 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD SUM PY 1995 VL 7 IS 3 BP 334 EP 337 PG 4 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA RL779 UT WOS:A1995RL77900010 PM 7580193 ER PT J AU SKATRUD, JB MORGAN, B AF SKATRUD, JB MORGAN, B TI HYPERTENSION AND SLEEP-APNEA SO JOURNAL OF SLEEP RESEARCH LA English DT Article DE ASPHYXIA; AUTONOMIC NERVOUS SYSTEM; CAROTID CHEMOREFLEX; HYPERTENSION; HYPOXIA; SLEEP APNEA; SYMPATHETIC NERVOUS SYSTEM ID APNEA; PRESSURE; HYPOXIA AB Epidemiological data indicate a link between sleep-disordered breathing and elevation of arterial pressure. Previous studies suggest increased activity of the sympathetic nervous system in patients with sleep apnoea. The response of muscle sympathetic nerve activity was further investigated in normal, awake subjects following exposure to 20 minutes of asphyxia. Sympathetic nerve traffic increased during exposure and remained elevated even after the return to room air breathing. These findings raise the possibility that this sustained elevation of sympathetic nerve traffic could play a role in the development of daytime hypertension in patients with sleep-disordered breathing. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT KINESIOL,MADISON,WI. RP SKATRUD, JB (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 11 TC 3 Z9 3 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA OSNEY MEAD, OXFORD, OXON, ENGLAND OX2 0EL SN 0962-1105 J9 J SLEEP RES JI J. Sleep Res. PD JUN PY 1995 VL 4 SU 1 BP 34 EP 36 PG 3 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA RK094 UT WOS:A1995RK09400007 ER PT J AU BRIONES, ER IBER, FL AF BRIONES, ER IBER, FL TI LIVER AND BILIARY-TRACT CHANGES AND INJURY ASSOCIATED WITH TOTAL PARENTERAL-NUTRITION - PATHOGENESIS AND PREVENTION SO JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION LA English DT Article DE TOTAL PARENTERAL NUTRITION; HEPATIC COMPLICATIONS; CHOLESTASIS; STEATOSIS; NUTRIENT DEFICIENCY; GALLSTONES ID INFLAMMATORY BOWEL-DISEASE; ESCHERICHIA-COLI ENDOTOXIN; CRITICALLY ILL PATIENTS; SERUM HEPATIC ENZYME; PERFUSED RAT-LIVER; BACTERIAL TRANSLOCATION; CARNITINE DEFICIENCY; LIPID EMULSIONS; FUNCTION TESTS; INTESTINAL TRANSPLANTATION AB Total parenteral nutrition (TPN), now widely used, is successful in preventing and reversing malnutrition in individuals with various diseases and conditions. However, hepatic and biliary complications of TPN are encountered in both adult and pediatric patients. Certain complications, such as sepsis and TPN-associated cholestasis, occur more frequently in very young infants. Continuing problems commonly seen in adults are steatosis and steatonecrosis. Reasons for the development of these complications are multifactorial. Etiologies of hepatic complications, especially the role of deficiency/excess of nutrients in the pathogenesis of hepatobiliary disorders, are summarized. Complications caused by the duration of TPN are discussed with emphasis on prevention and management. Evidence now suggests that prompt enteral feeding, even in minimal amounts, may prevent many of the metabolic complications associated with TPN. TPN should be used only in amounts meeting needs and for a duration essential to survival. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,GASTROENTEROL SERV,HINES,IL 60141. UNIV ILLINOIS,CHICAGO,IL. NR 116 TC 34 Z9 37 U1 2 U2 2 PU AMER COLL NUTRITION PI NEW YORK PA C/O HOSP. JOINT DIS. 301 E. 17TH ST., NEW YORK, NY 10003 SN 0731-5724 J9 J AM COLL NUTR JI J. Am. Coll. Nutr. PD JUN PY 1995 VL 14 IS 3 BP 219 EP 228 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA RB237 UT WOS:A1995RB23700005 PM 8586769 ER PT J AU GERETY, MB AF GERETY, MB TI HEALTH-CARE REFORM - BENEFITS OR HAZARDS FOR THE FRAIL AND THEIR DOCTORS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Editorial Material C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. RP GERETY, MB (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,GRECC 116C,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 7 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JUN PY 1995 VL 43 IS 6 BP 718 EP 719 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA RB444 UT WOS:A1995RB44400024 PM 7775738 ER PT J AU BISSADA, NK KACZMAREK, AT AF BISSADA, NK KACZMAREK, AT TI COMPLETE REMISSION OF HORMONE-REFRACTORY ADENOCARCINOMA OF THE PROSTATE IN RESPONSE TO WITHDRAWAL OF DIETHYLSTILBESTROL SO JOURNAL OF UROLOGY LA English DT Note DE DIETHYLSTILBESTROL; PROSTATE; ADENOCARCINOMA; ANDROGEN ANTAGONISTS ID FLUTAMIDE WITHDRAWAL; COMBINATION THERAPY; CANCER AB The phenomenon of regression of adenocarcinoma of the prostate after the withdrawal of antiandrogens is well documented. However, to our knowledge we report the first case of durable complete remission of hormone refractory prostate cancer after cessation of diethylstilbestrol. The drug was discontinued because the patient had disease progression while on diethylstilbestrol and withdrawal resulted in durable remission. In more than 3 years of followup since discontinuing diethylstilbestrol there has been no evidence of clinical or biochemical recurrence. C1 RALPH H JOHNSON VET ADM HOSP,CHARLESTON,SC. RP BISSADA, NK (reprint author), MED UNIV S CAROLINA,DEPT UROL,CHARLESTON,SC 29425, USA. NR 13 TC 47 Z9 47 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD JUN PY 1995 VL 153 IS 6 BP 1944 EP 1945 DI 10.1016/S0022-5347(01)67364-6 PG 2 WC Urology & Nephrology SC Urology & Nephrology GA QX369 UT WOS:A1995QX36900064 PM 7752364 ER PT J AU TATERKA, J CEBRA, JJ RUBIN, DH AF TATERKA, J CEBRA, JJ RUBIN, DH TI CHARACTERIZATION OF CYTOTOXIC-CELLS FROM REOVIRUS-INFECTED SCID MICE - ACTIVATED CELLS EXPRESS NATURAL KILLER-LIKE AND LYMPHOKINE-ACTIVATED KILLER-LIKE ACTIVITY BUT FAIL TO CLEAR INFECTION SO JOURNAL OF VIROLOGY LA English DT Note ID SEVERE COMBINED IMMUNODEFICIENCY; T-CELL; NK CELLS; LIVER; MOUSE; MYOCARDITIS; LYMPHOCYTES; SURFACE; DISEASE AB Severe combined immune deficient (SCID) mice infected orally with reovirus type 1/L die of hepatitis. Leukocytes bearing the cell surface antigens Thy-1.2 and asialoGM-1 (AsGM1) accumulate in the livers of infected animals. These cells display lytic activity toward natural killer-sensitive (YAC-1) and -resistant (P815) cell lines and murine hepatoma line Hepa 1/A1. Although these cells have the capacity to lyse infected hepatoma targets, they cannot clear the virus. C1 VET AFFAIRS MED CTR,DEPT MED,DIV GASTROENTEROL,PHILADELPHIA,PA. VET AFFAIRS MED CTR,DEPT BIOL,PHILADELPHIA,PA. UNIV PENN,DEPT MICROBIOL,PHILADELPHIA,PA 19104. FU NIAID NIH HHS [AI-23970]; NIDDK NIH HHS [T32-DK-07066] NR 27 TC 7 Z9 7 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JUN PY 1995 VL 69 IS 6 BP 3910 EP 3914 PG 5 WC Virology SC Virology GA QX931 UT WOS:A1995QX93100082 PM 7745745 ER PT J AU MORENS, DM GRANDINETTI, A REED, D WHITE, LR ROSS, GW AF MORENS, DM GRANDINETTI, A REED, D WHITE, LR ROSS, GW TI CIGARETTE-SMOKING AND PROTECTION FROM PARKINSONS-DISEASE - FALSE ASSOCIATION OR ETIOLOGIC CLUE SO NEUROLOGY LA English DT Review ID ENVIRONMENTAL RISK-FACTORS; DIAGNOSED ALZHEIMERS-DISEASE; MONOAMINE OXIDASE-B; ALCOHOL-CONSUMPTION; OLFACTORY FUNCTION; YOUNG-ONSET; NICOTINE; DOPAMINE; DEMENTIA; HYPOTHESIS AB We reviewed 46 published reports associating cigarette smoking and Parkinson's disease. Although the majority indicated an approximate halving of smoking frequency in persons with Parkinson's disease, many observers have suggested that the effect could be a spurious result. That the association may be real is suggested by at least six observations: (1) the consistency of findings between independent studies of different design, conducted by different investigators, in different nations, over 35 years; (2) the association's predominance and strength in prospective studies; (3) the apparent detection of a dose-response relation; (4) the inability to explain the association by confounding variables; (5) the flaws in certain arguments against the association's validity; and (6) the identification of a similar association, of similar magnitude, between smoking and reduced occurrence of Alzheimer's disease. A protective association of cigarette smoking for Parkinson's disease may constitute an important etiologic clue. C1 UNIV HAWAII,SCH MED,DEPT TROP MED,HONOLULU,HI 96822. EAST WEST CTR,HONOLULU,HI 96848. BUCK CTR RES AGING,NOVATA,CA. NIA,BETHESDA,MD 20892. NIA,HONOLULU ASIA AGING STUDY,HONOLULU,HI. US DEPT VET AFFAIRS,HONOLULU,HI. RP MORENS, DM (reprint author), UNIV HAWAII,SCH PUBL HLTH,DEPT PUBL HLTH SCI,BIOMED D103,1960 E W RD,HONOLULU,HI 96822, USA. FU NINDS NIH HHS [R01 NS 30371] NR 107 TC 279 Z9 287 U1 0 U2 7 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD JUN PY 1995 VL 45 IS 6 BP 1041 EP 1051 PG 11 WC Clinical Neurology SC Neurosciences & Neurology GA RC993 UT WOS:A1995RC99300002 PM 7783862 ER PT J AU BRANNAN, SK MILLER, A JONES, DJ KRAMER, GL PETTY, F AF BRANNAN, SK MILLER, A JONES, DJ KRAMER, GL PETTY, F TI BETA-ADRENERGIC-RECEPTOR CHANGES IN LEARNED HELPLESSNESS MAY DEPEND ON STRESS AND TEST PARAMETERS SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Note DE BETA-ADRENERGIC RECEPTOR; LEARNED HELPLESSNESS; DEPRESSION; NOREPINEPHRINE; ANIMAL MODELS ID INESCAPABLE SHOCK; OLFACTORY BULBECTOMY; MONOAMINE RECEPTORS; ANIMAL-MODELS; RAT-BRAIN; IMIPRAMINE; DEPRESSION; DEFICITS; AMYGDALA; ESCAPE AB Behavioral deficits following inescapable stress (learned helplessness) may serve as an animal model of depression. Previous studies using foot-shock stress to induce learned helplessness and a bar-press test for the stress-induced behavioral deficit have found increased beta-adrenergic receptor density in the hippocampus of learned helpless rats. We replicated these experiments using a tail-shock stress and the shuttle-box test. In our experiments, rats that developed learned helplessness after inescapable stress did not demonstrate any significant differences in beta-adrenergic receptor density or affinity in the frontal cortex, cerebellum, or hippocampus compared to the nonhelpless rats, nor to the tested control rats. These results suggest that beta-adrenergic receptor changes in learned helplessness may depend on the specific stress and test procedures used. C1 VET AFFAIRS MED CTR,PSYCHIAT SERV 116A,DALLAS,TX 75216. UNIV TEXAS,SW MED CTR,DEPT PSYCHIAT,DALLAS,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT ANESTHESIOL,SAN ANTONIO,TX 78284. NR 32 TC 13 Z9 13 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD JUN-JUL PY 1995 VL 51 IS 2-3 BP 553 EP 556 DI 10.1016/0091-3057(95)00054-Z PG 4 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA RB491 UT WOS:A1995RB49100063 PM 7667386 ER PT J AU SWARTZ, BE AF SWARTZ, BE TI UNUSUAL SEIZURE TYPES SO SEMINARS IN NEUROLOGY LA English DT Article ID TEMPORAL-LOBE EPILEPSY; ORIGIN; CLASSIFICATION; STIMULATION; PARIETAL; SURGERY C1 UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. RP SWARTZ, BE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CTR EPILEPSY W127B,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 61 TC 3 Z9 4 U1 0 U2 0 PU THIEME MEDICAL PUBL INC PI NEW YORK PA 381 PARK AVE SOUTH, NEW YORK, NY 10016 SN 0271-8235 J9 SEMIN NEUROL JI Semin. Neurol. PD JUN PY 1995 VL 15 IS 2 BP 151 EP 157 DI 10.1055/s-2008-1041018 PG 7 WC Clinical Neurology SC Neurosciences & Neurology GA RM956 UT WOS:A1995RM95600006 PM 7481134 ER PT J AU RAZDAN, K KROLL, MH AF RAZDAN, K KROLL, MH TI IDENTIFICATION OF A NOVEL PROTEIN-KINASE-C SUBSTRATE FROM PLATELETS AND CELLS OF MEGAKARYOCYTE LINEAGE SO THROMBOSIS AND HAEMOSTASIS LA English DT Meeting Abstract C1 BAYLOR COLL MED,VA MED CTR,HOUSTON,TX 77030. RICE UNIV,HOUSTON,TX 77251. NR 0 TC 0 Z9 0 U1 0 U2 0 PU F K SCHATTAUER VERLAG GMBH PI STUTTGART PA P O BOX 10 45 45, LENZHALDE 3, D-70040 STUTTGART, GERMANY SN 0340-6245 J9 THROMB HAEMOSTASIS JI Thromb. Haemost. PD JUN PY 1995 VL 73 IS 6 BP 988 EP 988 PG 1 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA RP385 UT WOS:A1995RP38500339 ER PT J AU OHLIN, AK MARLAR, RA AF OHLIN, AK MARLAR, RA TI MUTATIONS IN THE THROMBOMODULIN GENE ASSOCIATED WITH THROMBOEMBOLIC DISEASE SO THROMBOSIS AND HAEMOSTASIS LA English DT Meeting Abstract C1 UNIV LUND HOSP,DEPT CLIN CHEM,S-22185 LUND,SWEDEN. UNIV COLORADO,SCH MED,DEPT PATHOL,BOULDER,CO 80309. DENVER VET AFFAIRS MED CTR,DENVER,CO 80220. NR 0 TC 7 Z9 7 U1 0 U2 0 PU F K SCHATTAUER VERLAG GMBH PI STUTTGART PA P O BOX 10 45 45, LENZHALDE 3, D-70040 STUTTGART, GERMANY SN 0340-6245 J9 THROMB HAEMOSTASIS JI Thromb. Haemost. PD JUN PY 1995 VL 73 IS 6 BP 1096 EP 1096 PG 1 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA RP385 UT WOS:A1995RP38500748 ER PT J AU ADAMI, C POOLEY, J GLOMB, J STECKER, E FAZAL, F FLEMING, JO BAKER, SC AF ADAMI, C POOLEY, J GLOMB, J STECKER, E FAZAL, F FLEMING, JO BAKER, SC TI EVOLUTION OF MOUSE HEPATITIS-VIRUS (MHV) DURING CHRONIC INFECTION - QUASI-SPECIES NATURE OF THE PERSISTING MHV RNA SO VIROLOGY LA English DT Article ID DOUBLE-STRANDED-RNA; SUBACUTE SCLEROSING PANENCEPHALITIS; MURINE CORONAVIRUS JHM; BIASED HYPERMUTATION; UNWINDING ACTIVITY; MEASLES-VIRUS; C VIRUS; VIRAL PERSISTENCE; SEQUENCE-ANALYSIS; RAPID EVOLUTION AB Coronavirus infection of mice has been used extensively as a model for the study of acute encephalitis and chronic demyelination. To examine the evolution of coronavirus RNA during chronic demyelinating infection, we isolated RNA from intracerebrally inoculated mice at 4, 6, 8, 13, 20, and 42 days postinfection and used reverse transcription-polymerase chain reaction amplification methods (RT-PCR) to detect viral sequences. RNA sequences from two viral structural genes, the spike gene and the nucleocapsid gene, were detected throughout the chronic infection. In contrast, infectious virus was not detectable from brain homongenates beyond 13 days postinfection. These results indicate that coronavirus RNA persists in the brain at times when infectious virus is not detected. To determine if genetic changes were occurring during viral replication in the host, we cloned and sequenced the RT-PCR products from the spike and nucleocapsid regions and analyzed the sequences for mutation's. Sequencing of the cloned products revealed that a variety of mutant forms of viral RNA persisted in the CNS, including point mutants, deletion mutants, and termination mutants. The mutations accumulated during persistent infection in both the spike and the nucleocapsid sequences, with greater than 65% of the mutations encoding amino acid changes. These results show that a diverse population or quasispecies consisting of mutant and deletion variant Viral RNAs (which may not be capable of producing infectious virus particles) persists in the central nervous system of mice during chronic demyelinating infection. The implications of these results for the role of persistent viral genetic information in the pathogenesis of chronic demyelination are discussed. (C) 1995 Academic Press, Inc. C1 LOYOLA UNIV,STRITCH SCH MED,DEPT MICROBIOL & IMMUNOL,MAYWOOD,IL 60153. UNIV WISCONSIN,DEPT NEUROL,MADISON,WI 53792. UNIV WISCONSIN,DEPT MED MICROBIOL,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53792. FU NIAID NIH HHS [AI32065] NR 37 TC 73 Z9 75 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0042-6822 J9 VIROLOGY JI Virology PD JUN 1 PY 1995 VL 209 IS 2 BP 337 EP 346 DI 10.1006/viro.1995.1265 PG 10 WC Virology SC Virology GA RC351 UT WOS:A1995RC35100007 PM 7778268 ER PT J AU RAWSON, NE BRAND, JG COWART, BJ LOWRY, LD PRIBITKIN, EA RAO, VM RESTREPO, D AF RAWSON, NE BRAND, JG COWART, BJ LOWRY, LD PRIBITKIN, EA RAO, VM RESTREPO, D TI FUNCTIONALLY MATURE OLFACTORY NEURONS FROM 2 ANOSMIC PATIENTS WITH KALLMANN-SYNDROME SO BRAIN RESEARCH LA English DT Article DE OLFACTION; CALCIUM; HYPOGONADOTROPIC HYPOGONADISM ID 2ND MESSENGER PATHWAYS; BULBECTOMY; RECEPTOR; CILIA; CONNECTIONS; ODORANTS; HYPOGONADISM; STIMULATION; RESPONSES; FOREBRAIN AB Patients with Kallmann syndrome (KS) exhibit hypogonadotropic hypogonadism and anosmia [Kallmann et al., Am. J. Mental Def., 48 (1944) 203-236] secondary to failure of gonadotropin-releasing hormone (GnRH)-producing neurons to migrate from the olfactory placode to the brain, and to agenesis of the olfactory bulbs. It has been hypothesized that olfactory neurons (ON) from individuals with KS are immature partly on the basis of studies in animals showing that lack of synaptic connection of ON with the olfactory bulb results in expression of immature ON [Schwob et al., J. Neurosci, 12 (1979) 880-883]. To test this assumption, we obtained olfactory tissue samples from two males diagnosed with KS on the basis of medical history and MRI studies. Both patients were anosmic. The functioning of cells isolated from biopsies taken from the upper middle turbinate and septum was studied by measuring changes in intracellular Ca2+ concentration ([Ca-i]) using dual excitation fluorescence microscopy. Biopsies from both patients yielded cells that morphologically appeared to be ON. Seven of 16 cells that morphologically resembled ON responded with a change in [Ca-i] upon stimulation with an odorant mixture. These studies show that at least some ON in KS individuals are functionally mature and suggest that complete development of the olfactory bulbs is not required for differentiation of mature human ON. C1 THOMAS JEFFERSON UNIV, PHILADELPHIA, PA 19107 USA. VET AFFAIRS MED CTR, PHILADELPHIA, PA USA. UNIV PENN, PHILADELPHIA, PA 19104 USA. RP MONELL CHEM SENSES CTR, 3500 MARKET ST, PHILADELPHIA, PA 19104 USA. FU NIDCD NIH HHS [DC-00014, DC-00214, DC-00566] NR 43 TC 29 Z9 29 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 EI 1872-6240 J9 BRAIN RES JI Brain Res. PD MAY 29 PY 1995 VL 681 IS 1-2 BP 58 EP 64 DI 10.1016/0006-8993(95)00283-V PG 7 WC Neurosciences SC Neurosciences & Neurology GA RD895 UT WOS:A1995RD89500007 PM 7552292 ER PT J AU CASINI, G RICKMAN, DW BRECHA, NC AF CASINI, G RICKMAN, DW BRECHA, NC TI AII AMACRINE CELL-POPULATION IN THE RABBIT RETINA - IDENTIFICATION BY PARVALBUMIN IMMUNOREACTIVITY SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE NARROW-FIELD AMACRINE CELL; CALCIUM-BINDING PROTEIN; ROD PATHWAY; DOPAMINERGIC AMACRINE CELL ID INNER PLEXIFORM LAYER; CALCIUM-BINDING PROTEINS; CAT RETINA; MAMMALIAN RETINA; RAT RETINA; QUANTITATIVE-ANALYSIS; POSTNATAL-DEVELOPMENT; ACCUMULATING NEURONS; SYNAPTIC CONNECTIONS; VERTEBRATE RETINA AB Parvalbumin (PV) is a calcium-binding protein localized to selected neurons in the nervous system, including the retina. This investigation evaluated the distribution of PV immunoreactivity in the rabbit retina using immunohistochemistry with a monoclonal antibody directed to carp PV. In the inner nuclear layer (INL), PV immunoreactivity was present in horizontal and amacrine cells. In the ganglion cell layer, PV immunostaining was confined to somata that are likely to be both displaced amacrine cells and ganglion cells. PV-immunoreactive (IR) amacrine cells were positioned in the proximal INL adjacent to the inner plexiform layer (IPL). These cells usually gave rise to a single primary process, which arborized into two distinct bands in the IPL. In sublamina a, the processes were thin and had large, irregular endings. In sublamina b, multiple processes branched from the primary process and were characterized by varicosities and spines. PV-IR amacrine cell bodies measured from 8 to 10 mu m in diameter. Their density was highest in the visual streak and lowest in the periphery of the superior retina. The average number of PV-IR amacrine cells was 464,045 cells per retina (N = 3), and the average regularity index of the PV-IR cell mosaic was 3.23. PV-IR amacrine cells were further characterized by double-label immunofluorescence experiments using antibodies to PV and tyrosine hydroxylase (TH). Varicose TH-IR processes were in close apposition to many PV-IR amacrine cells and often formed ''ring structures'' around them. Together, these morphological, quantitative, and histochemical observations indicate that PV immunoreactivity in the INL is localized predominantly to AII amacrine cells, and therefore it is a valuable marker for the identification of this cell type. (C) 1995 Wiley-Liss, Inc. C1 W LOS ANGELES VET AFFAIRS MED CTR,CURE,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,VA,CURE,CTR GASTROENTER BIOL,BRAIN RES INST,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,VA,CURE,CTR GASTROENTER BIOL,BRAIN RES INST,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,JULES STEIN EYE INST,LOS ANGELES,CA 90024. FU NEI NIH HHS [R01 EY004067, EY 04067] NR 47 TC 54 Z9 55 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD MAY 22 PY 1995 VL 356 IS 1 BP 132 EP 142 DI 10.1002/cne.903560109 PG 11 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA RA886 UT WOS:A1995RA88600008 PM 7629307 ER PT J AU LI, JJ OBERLEY, LW STCLAIR, DK RIDNOUR, LA OBERLEY, TD AF LI, JJ OBERLEY, LW STCLAIR, DK RIDNOUR, LA OBERLEY, TD TI PHENOTYPIC CHANGES INDUCED IN HUMAN BREAST-CANCER CELLS BY OVEREXPRESSION OF MANGANESE-CONTAINING SUPEROXIDE-DISMUTASE SO ONCOGENE LA English DT Article DE BREAST; CANCER; MNSOD; TRANSFECTION; SOFT AGAR; NUDE MICE ID SYRIAN-HAMSTER TISSUES; GLUTATHIONE-PEROXIDASE; ANTIOXIDANT ENZYMES; IMMUNOHISTOCHEMICAL LOCALIZATION; KIDNEY DEVELOPMENT; TRANSFECTED CELLS; EXPRESSION VECTOR; TRANSGENIC MICE; MAMMALIAN-CELLS; DOWNS-SYNDROME AB Human manganese containing superoxide dismutase (MnSOD) cDNA was transfected into a human breast cancer cell line (MCF-7) in order to examine the effect of increased functional MnSOD on the cellular phenotype. A MnSOD-overexpressing clone was compared to control vector-transfected cells and to wild type MCF-7 cells. Southern blotting indicated incorporation of MnSOD cDNA into genomic DNA in the MnSOD overexpressing cell line. The MnSOD overexpressing cell line showed a 5.7-fold increase in MnSOD activity compared to wild type MCF-7 cells. Similar increases in MnSOD immunoreactive protein and mRNA levels were observed by Western and Northern blotting as well as using RT-PCR, The plating efficiency of cells grown in different concentrations of serum (1 to 20%) was decreased in the MnSOD overexpressing cell line. The fraction in soft agar culture was also after MnSOD cDNA transfection. When inoculated in nude mice, tumor growth was markedly inhibited in MnSOD overexpressing cells compared to wild type MCF-7 cells or plasmid control cells, These results support the hypothesis that increased MnSOD expression suppresses the malignant phenotype of human breast cancer cells and suggests that the MnSOD gene is a tumor suppressor gene in human breast cancer. C1 UNIV IOWA,RADIAT RES LAB,MED LABS 14,IOWA CITY,IA 52242. UNIV KENTUCKY,GRAD CTR TOXICOL,LEXINGTON,KY 40506. WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL SERV,MADISON,WI 53705. FU NCI NIH HHS [CA 41267, CA 49797] NR 49 TC 230 Z9 234 U1 0 U2 4 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HANTS, ENGLAND RG21 2XS SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAY 18 PY 1995 VL 10 IS 10 BP 1989 EP 2000 PG 12 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA QZ926 UT WOS:A1995QZ92600013 PM 7761099 ER PT J AU PROVINCE, MA HADLEY, EC HORNBROOK, MC LIPSITZ, LA MULROW, CD ORY, MG SATTIN, RW TINETTI, ME WOLF, SL AF PROVINCE, MA HADLEY, EC HORNBROOK, MC LIPSITZ, LA MULROW, CD ORY, MG SATTIN, RW TINETTI, ME WOLF, SL TI THE EFFECTS OF EXERCISE ON FALLS IN ELDERLY PATIENTS - A PREPLANNED METAANALYSIS OF THE FICSIT TRIALS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID FUNCTIONAL STATUS; OLDER PERSONS; RISK-FACTORS; POPULATION; FRAILTY; PREDICTORS; COMMUNITY; INJURIES; BALANCE; PEOPLE AB Objective.-To determine if short-term exercise reduces falls and fall-related injuries in the elderly. Design.-A preplanned meta-analysis of the seven Frailty and Injuries: Cooperative Studies of Intervention Techniques (FICSIT)-independent, randomized, controlled clinical trials that assessed intervention efficacy in reducing falls and frailty in elderly patients. All included an exercise component for 10 to 36 weeks. Fall and injury follow-up was obtained for up to 2 to 4 years. Setting.-Two nursing home and five community-dwelling (three health maintenance organizations) sites. Six were group and center based; one was conducted at home. Participants.-Numbers of participants ranged from 100 to 1323 per study. Subjects were mostly ambulatory and cognitively intact, with minimum ages of 60 to 75 years, although some studies required additional deficits, such as functionally dependent in two or more activities of daily living, balance deficits or lower extremity weakness, or high risk of falling. Interventions.-Exercise components varied across studies in character, duration, frequency, and intensity. Training was performed in one area or more of endurance, flexibility, balance platform, Tai Chi (dynamic balance), and resistance. Several treatment arms included additional nonexercise components, such as behavioral components, medication changes, education, functional activity, or nutritional supplements. Main Outcome Measures.-Time to each fall (fall-related injury) by self-report and/or medical records. Results.-Using the Andersen-Gill extension of the Cox model that allows multiple fall outcomes per patient, the adjusted fall incidence ratio for treatment arms including general exercise was 0.90 (95% confidence limits [CL], 0.81, 0.99) and for those including balance was 0.83 (95% CL, 0.70, 0.98). No exercise component was significant for injurious falls, but power was low to detect this outcome. Conclusions.-Treatments including exercise for elderly adults reduce the risk of falls. C1 NIA,BETHESDA,MD 20892. KAISER PERMANENTE CTR HLTH RES,PORTLAND,OR. HARVARD UNIV,BETH ISRAEL HOSP,HEBREW REHABIL CTR AGED,BOSTON,MA 02215. AUDIE L MURPHY MEM VET ADM MED CTR,DEPT MED,SAN ANTONIO,TX. CTR DIS CONTROL & PREVENT,ATLANTA,GA 30341. YALE UNIV,SCH MED,PROGRAM AGING,NEW HAVEN,CT. EMORY UNIV,SCH MED,DEPT REHABIL MED,ATLANTA,GA. RP PROVINCE, MA (reprint author), WASHINGTON UNIV,SCH MED,DIV BIOSTAT,BOX 8067,600 S EUCLID ST,ST LOUIS,MO 63110, USA. RI Wolf, Steven/F-6588-2010 OI Wolf, Steven/0000-0002-9446-8995; Miller, J Philip/0000-0003-4568-6846 NR 44 TC 643 Z9 658 U1 25 U2 82 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAY 3 PY 1995 VL 273 IS 17 BP 1341 EP 1347 DI 10.1001/jama.273.17.1341 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA QV307 UT WOS:A1995QV30700018 PM 7715058 ER PT J AU KOSOWER, E AF KOSOWER, E TI AN ASSESSMENT AND TEACHING TOOL FOR INTERPERSONAL-COMMUNICATION SKILLS SO ACADEMIC MEDICINE LA English DT Note C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VAMC,LOS ANGELES,CA 90024. NR 0 TC 3 Z9 3 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD MAY PY 1995 VL 70 IS 5 BP 452 EP 453 DI 10.1097/00001888-199505000-00056 PG 2 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA QZ280 UT WOS:A1995QZ28000062 PM 7748425 ER PT J AU CHEN, RYZ GUTH, PH AF CHEN, RYZ GUTH, PH TI INTERACTION OF ENDOGENOUS NITRIC-OXIDE AND CGRP IN SENSORY NEURON-INDUCED GASTRIC VASODILATION SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE GASTRIC MICROCIRCULATION; CAPSAICIN-SENSITIVE SENSORY NERVES; CALCITONIN GENE-RELATED PEPTIDE; HUMAN CALCITONIN GENE-RELATED PEPTIDE-(8-37) ID GENE-RELATED PEPTIDE; ACID BACK-DIFFUSION; MUCOSAL BLOOD-FLOW; INTRAGASTRIC CAPSAICIN; HYPEREMIC RESPONSE; MESENTERIC-ARTERY; NERVOUS-SYSTEM; BINDING-SITES; RAT STOMACH; CALCITONIN AB Stimulation of capsaicin-sensitive sensory nerves induces gastric mucosal hyperemia, which is mediated in part by both calcitonin gene-related peptide (CGRP) and nitric oxide (NO). In the present study, we used in vivo microscopy in anesthetized rats to determine 1) whether these agents were released locally at the submucosal level and, if so, 2) whether CGRP dilates arterioles via release of endothelium-derived NO. Intragastric capsaicin (160 mu M) dilated submucosal arterioles from 25 +/- 3 to 67 +/- 8 mu m. The intragastric capsaicin-induced vasodilation was markedly reversed not only by intravenous administration of the NO synthesis inhibitor N-G-nitro-L-arginine methyl ester (L-NAME) but also by submucosal suffusion of either L-NAME or the CORP receptor antagonist human CGRP-(8-37). The latter findings indicate that both NO and CGRP are released locally at the submucosal level. Submucosal application of CGRP induced dose-dependent dilation of gastric submucosal arterioles, which was significantly attenuated by L-NAME. However, at the same degree of vasodilation (42 mu m), the dilation induced with submucosal CGRP was much less attenuated by NO synthesis inhibition (-28%) compared with that induced with intragastric capsaicin (-79%). This indicates that endothelium-derived NO released by CGRP was not the only source of submucosal NO in the latter response. There must be another as yet undetermined source of submucosal NO, e.g., possibly nitroxidergic nerves. C1 W LOS ANGELES VET AFFAIRS MED CTR, MED SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, RES SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, LOS ANGELES, CA 90073 USA. RP CHEN, RYZ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, DEPT ANESTHESIOL W212, ANESTHESIOL SERV, LOS ANGELES, CA 90073 USA. NR 31 TC 27 Z9 28 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD MAY PY 1995 VL 268 IS 5 BP G791 EP G796 PG 6 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA QW835 UT WOS:A1995QW83500009 PM 7762663 ER PT J AU ZILE, MR MUKHERJEE, R CLAYTON, C KATO, S SPINALE, FG AF ZILE, MR MUKHERJEE, R CLAYTON, C KATO, S SPINALE, FG TI EFFECTS OF CHRONIC SUPRAVENTRICULAR PACING TACHYCARDIA ON RELAXATION RATE IN ISOLATED CARDIAC-MUSCLE-CELLS SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE DIASTOLE; CARDIOMYOPATHY; ISOLATED CARDIOCYTE ID INDUCED HEART-FAILURE; FORCE-FREQUENCY-RELATIONSHIP; BETA-ADRENERGIC STIMULATION; INDUCED CARDIOMYOPATHY; VENTRICULAR MYOCYTES; DIASTOLIC FUNCTION; MYOCARDIUM; RECOVERY; DOGS; TEMPERATURE AB Chronic supraventricular pacing tachycardia (SVT) causes abnormalities in both ventricular and cellular relaxation. The mechanisms causing these abnormalities have not been fully determined. To examine two of the possible mechanisms, a decrease in restoring force or an impairment of the intrinsic myocardial relaxation process, cardiocytes were enzymatically isolated from the left ventricle of pigs subjected to left atrial pacing at 240 beats/min for 3 wk and normal control pigs. SVT caused a decrease in the extent of cardiocyte shortening and the velocity of cardiocyte lengthening. To determine whether the changes in the relaxation velocity merely reflected a concomitant decrease in the extent of cardiocyte shortening (and a resultant decrease in restoring forces) or, in addition, reflected impairment in intrinsic relaxation properties, the relation between cardiocyte relaxation velocity and cardiocyte shortening extent was examined. There was a direct relation between relaxation velocity and shortening extent in both control and SVT cardiocytes. However, SVT decreased the relaxation velocity at any common extent of shortening and decreased the slope of the direct relation (slope 5.91 in control vs. 3.51 s(-1) in SVT, P < 0.05). Therefore, these data suggested that SVT caused a primary impairment in the intrinsic myocardial relaxation process independently of a decrease in restoring force. C1 RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, CHARLESTON, SC 29425 USA. RP ZILE, MR (reprint author), MED UNIV S CAROLINA, GAZES CARDIAC RES INST, DEPT MED, DEPT SURG, DIV CARDIOL, CHARLESTON, SC 29425 USA. NR 44 TC 20 Z9 20 U1 0 U2 2 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD MAY PY 1995 VL 268 IS 5 BP H2104 EP H2113 PG 10 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA QW850 UT WOS:A1995QW85000040 ER PT J AU DUERINCKX, AJ BOGAERT, J JIANG, H LEWIS, BS AF DUERINCKX, AJ BOGAERT, J JIANG, H LEWIS, BS TI ANOMALOUS ORIGIN OF THE LEFT CORONARY-ARTERY - DIAGNOSIS BY CORONARY MR-ANGIOGRAPHY SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Note ID DEFINITION; SINUS C1 UNIV CALIF LOS ANGELES,MED CTR,DEPT RADIOL,LOS ANGELES,CA 90024. CATHOLIC UNIV LEUVEN,DEPT RADIOL,B-3000 LOUVAIN,BELGIUM. W LOS ANGELES VET AFFAIRS MED CTR,CARDIOL SECT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90024. RP DUERINCKX, AJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,SERV RADIOL,MAIL ROUTE W114,MRI,BLDG 507,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. RI bogaert, jan/E-6181-2012 NR 9 TC 28 Z9 29 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD MAY PY 1995 VL 164 IS 5 BP 1095 EP 1097 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA QT936 UT WOS:A1995QT93600007 PM 7717210 ER PT J AU GROSS, ME GIRON, KP SEPTIMUS, JD MASON, EO MUSHER, DM AF GROSS, ME GIRON, KP SEPTIMUS, JD MASON, EO MUSHER, DM TI ANTIMICROBIAL ACTIVITIES OF BETA-LACTAM ANTIBIOTICS AND GENTAMICIN AGAINST PENICILLIN-SUSCEPTIBLE AND PENICILLIN-RESISTANT PNEUMOCOCCI SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Note ID STREPTOCOCCUS-PNEUMONIAE; MENINGITIS; THERAPY; INVIVO AB The MICs of penicillin and cefotaxime for a range of penicillin-susceptible and penicillin-resistant isolates of Streptococcus pneumoniae were unchanged by the addition of gentamicin. In time-kill studies the rate of killing was greater for 18 of 20 isolates in the presence of gentamicin. However, mean differences in killing after 6 h of incubation were modest, not exceeding 1 log(10) unit. C1 DEPT VET AFFAIRS MED CTR,INFECT DIS SECT,MED SERV,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT PEDIAT,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MICROBIOL IMMUNOL,HOUSTON,TX 77030. NR 10 TC 19 Z9 19 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD MAY PY 1995 VL 39 IS 5 BP 1166 EP 1168 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA QW019 UT WOS:A1995QW01900026 PM 7625807 ER PT J AU SIEBERT, JD MCCLURE, SP BANKS, PM GULLEY, ML AF SIEBERT, JD MCCLURE, SP BANKS, PM GULLEY, ML TI HODGKINS-DISEASE, MIXED CELLULARITY TYPE, WITH A B-CELL IMMUNOPHENOTYPE - REPORT OF A CASE AND LITERATURE-REVIEW SO ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE LA English DT Note ID REED-STERNBERG CELLS; IMMUNOGLOBULIN GENE REARRANGEMENTS; LYMPHOCYTE PREDOMINANCE TYPE; MONOCLONAL-ANTIBODIES; H VARIANTS; DIAGNOSIS; LYMPHOMAS; IMMUNOPEROXIDASE; EXPRESSION; ANTIGEN AB The origin of the Reed-Sternberg cell, the neoplastic cell of Hodgkin's disease, has not been defined. We evaluated a case of Hodgkin's disease, mixed cellularity type, which presented in the retroperitoneum of a 45-year-old woman. Reed-Sternberg cells and Hodgkin's cells expressed the characteristic markers CD15 and CD30. In addition, they expressed the B-cell antigens CD19 and CD20, as well as CD45/leukocyte common antigen. Clonal rearrangement of the immunoglobulin heavy chain gene was detected by Southern blot analysis. These results suggest that some cases of Hodgkin's disease ave derived from an activated cell of lymphoid origin. This case documents a close relationship between Hodgkin's disease and non-Hodgkin's lymphoma, and it demonstrates that even when newer ancillary techniques are employed these two entities can have overlapping features. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. CITY HOSP,DEPT PATHOL & LAB MED,AKRON,OH. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. NR 38 TC 7 Z9 7 U1 0 U2 0 PU COLLEGE AMER PATHOLOGISTS PI NORTHFIELD PA C/O KIMBERLY GACKI, 325 WAUKEGAN RD, NORTHFIELD, IL 60093-2750 SN 0003-9985 J9 ARCH PATHOL LAB MED JI Arch. Pathol. Lab. Med. PD MAY PY 1995 VL 119 IS 5 BP 474 EP 479 PG 6 WC Medical Laboratory Technology; Medicine, Research & Experimental; Pathology SC Medical Laboratory Technology; Research & Experimental Medicine; Pathology GA QX384 UT WOS:A1995QX38400023 PM 7538289 ER PT J AU HOWARD, JP DRAKE, TR KELLOGG, DL AF HOWARD, JP DRAKE, TR KELLOGG, DL TI EFFECTS OF ALTERNATING-CURRENT IONTOPHORESIS ON DRUG-DELIVERY SO ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION LA English DT Article ID THERAPEUTIC PEPTIDES; PROTEINS; DEVICES AB Objective: The duration of direct current (DC) iontophoresis is limited to 10- to 15-minute periods because of electrochemical burns from hydrogen and hydroxide ions generated by the DC current, A new iontophoretic device, the Lectro Patch, uses a low-frequency alternating current (AC), AC current is theorized to generate H+ ions during one phase and OH- when the current reverses polarity, thus possibly neutralizing pH changes and avoiding burns, This study examined this possibility and evaluated drug delivery with AC iontophoresis, using hydroxocobalamin. Design: A known amount of hydroxocobalamin dissolved in 6mL of water was loaded in Lectro Patches, two of which were then taped on the forearms of 10 patient volunteers, One patch was activated to deliver drug by AC iontophoresis, The second patch was not activated and served as a control for delivery by diffusion, Trials were run for 2 and 4 hours, with both 1,000 mu g/mL and 2,000 mu g/mL concentrations, Setting: Study was conducted with inpatients in an extended care setting using volunteers, Main Outcome Measures: Amounts of hydroxocobalamin remaining in the Lectro Patches after iontophoresis were assayed by spectrophotometry, Data were analyzed by ANOVA, Results: No burns occurred, Significantly greater losses occurred with 4 hours of iontophoresis than with 2 hours (p < 0.05), There was no significant effect of changing the concentration of hydroxocobalamin. Conclusions: AC iontophoresis avoids electrochemical burns; charged drugs can be delivered by AC iontophoresis; and delivery of drug increases with duration of application. (C) 1995 by the American Congress of Rehabilitation Medicine and the American Academy of Physical Medicine and Rehabilitation C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GERIATR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,GRECC,SAN ANTONIO,TX 78284. FU NIA NIH HHS [AG00230] NR 20 TC 26 Z9 26 U1 0 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0003-9993 J9 ARCH PHYS MED REHAB JI Arch. Phys. Med. Rehabil. PD MAY PY 1995 VL 76 IS 5 BP 463 EP 466 DI 10.1016/S0003-9993(95)80579-6 PG 4 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA QX808 UT WOS:A1995QX80800013 PM 7741619 ER PT J AU Whatley, VJ Brozowski, SJ Whiting, PJ Harris, RA AF Whatley, V. J. Brozowski, S. J. Whiting, P. J. Harris, R. A. TI Microtubule depolymerization disaggregates GABA(A) receptor clustering and inhibits GABA(A)ergic function in stably transfected cells SO BEHAVIOURAL PHARMACOLOGY LA English DT Meeting Abstract C1 Univ Colorado, Sch Med, Denver VAMC, Denver, CO 80262 USA. Univ Colorado, Sch Med, Dept Pharmacol, Denver, CO 80262 USA. Merck Sharp & Dohme Neurosci Res, Harlow, Essex, England. NR 4 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0955-8810 J9 BEHAV PHARMACOL JI Behav. Pharmacol. PD MAY PY 1995 VL 6 SU 1 BP 118 EP 118 DI 10.1097/00008877-199505001-00136 PG 1 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA V26ZP UT WOS:000208583800137 ER PT J AU YOVELL, Y SIEVER, L TRESTMAN, R MITROPOULOU, V ERDOS, J GELERNTER, J AF YOVELL, Y SIEVER, L TRESTMAN, R MITROPOULOU, V ERDOS, J GELERNTER, J TI TRYPTOPHAN-HYDROXYLASE POLYMORPHISM IS ASSOCIATED WITH IMPULSIVE AGGRESSION SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 MT SINAI SCH MED,BRONX,NY 10468. BRONX VET AFFAIRS MED CTR,BRONX,NY 10468. YALE UNIV,SCH MED,NEW HAVEN,CT 06516. W HAVEN VET AFFAIRS MED CTR,NEW HAVEN,CT 06516. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiat. PD MAY 1 PY 1995 VL 37 IS 9 BP 644 EP 644 DI 10.1016/0006-3223(95)94596-O PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QX037 UT WOS:A1995QX03700187 ER PT J AU BERGMAN, A ROITMAN, SL OSGOOD, G CORNBLATT, B SIEVER, L AF BERGMAN, A ROITMAN, SL OSGOOD, G CORNBLATT, B SIEVER, L TI ATTENTIONAL DYSFUNCTION IN SCHIZOTYPAL PERSONALITY-DISORDER SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 ST JOHNS UNIV,JAMAICA,NY. MT SINAI MED CTR,NEW YORK,NY 10029. BRONX VET ADM MED CTR,BRONX,NY 10468. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiat. PD MAY 1 PY 1995 VL 37 IS 9 BP 645 EP 645 DI 10.1016/0006-3223(95)94600-2 PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QX037 UT WOS:A1995QX03700191 ER PT J AU HAMNER, MB ARVANITIS, LA MILLER, BG LINK, C HONG, WW AF HAMNER, MB ARVANITIS, LA MILLER, BG LINK, C HONG, WW TI THE EFFECTS OF SEROQUEL (ICI-204.636) ON PLASMA PROLACTIN IN PATIENTS WITH SCHIZOPHRENIA SO BIOLOGICAL PSYCHIATRY LA English DT Meeting Abstract C1 RALPH H JOHNSON VA MED CTR,DEPT PSYCHIAT,CHARLESTON,SC 29401. ZENECA PHARMACEUT,CNS CLIN RES,WILMINGTON,DE 19897. NR 0 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiat. PD MAY 1 PY 1995 VL 37 IS 9 BP 683 EP 683 DI 10.1016/0006-3223(95)94734-E PG 1 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QX037 UT WOS:A1995QX03700325 ER PT J AU REDDY, SV KUZHANDAIVELU, N ACOSTA, LG ROODMAN, GD AF REDDY, SV KUZHANDAIVELU, N ACOSTA, LG ROODMAN, GD TI CHARACTERIZATION OF THE 5'-FLANKING REGION OF THE HUMAN TARTRATE-RESISTANT ACID-PHOSPHATASE (TRAP) GENE SO BONE LA English DT Article DE 5'-FLANKING SEQUENCE; TRANSCRIPTION FACTORS; TARTRATE-RESISTANT ACID PHOSPHATASE; PROMOTER; POLYMERASE CHAIN REACTION; INTRON ID TYPE-5; MOUSE; UTEROFERRIN; CLONING AB Tartrate-resistant acid phosphatase (TRAP) is expressed at high levels in osteoclasts and may play an important role in the bone resorptive process, However, factors regulating human TRAP gene expression have not been clearly defined. Therefore, we isolated a genomic clone (CL-9) for TRAP containing a 14-kb insert, A restriction map was generated for this insert, and a 2,6-kb ApaI fragment containing the 5'-flanking region was subcloned, Sequence analysis of this fragment revealed the presence of candidate transcription factor-binding sequences for H-APF-1, SP1, GATA2, and the c-Myc proto-oncogene, PCR analysis of RNA isolated from human osteoclastomas and pagetic bone revealed a 276-bp intron at -1 bp to -276 bp relative to the ATG and a transcript originating from this intron. Rapid amplification of the 5' end of the human TRAP mRNA by PCR indicated the presence of a 93-bp untranslated region 5' from the intron. Promoter activity was detected in the DNA fragment from +1 bp to -1903 bp relative to the ATG initiation codon, which drove the transient expression of a luciferase reporter gene when transfected into HRE H9 rabbit endometrial cells. Comparison of the human TRAP 5'-flanking region with mouse TRAP and uteroferrin revealed 41% and 47% homology, respectively. This suggests that regulation of human TRAP gene expression may differ from that for the murine TRAP gene. C1 UNIV TEXAS,AUDIE MURPHY VET ADM HOSP,HLTH SCI CTR,DEPT MED HEMATOL,RES SERV 151,SAN ANTONIO,TX 78284. FU NIADDK NIH HHS [AM 35188]; NIAMS NIH HHS [AR 41336, AR 39539] NR 18 TC 17 Z9 17 U1 0 U2 1 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 8756-3282 J9 BONE JI Bone PD MAY PY 1995 VL 16 IS 5 BP 587 EP 593 DI 10.1016/8756-3282(95)00086-S PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA RB640 UT WOS:A1995RB64000014 PM 7654474 ER PT J AU VIRELLA, G MUNOZ, JF GALBRAITH, GMP GISSINGER, C CHASSEREAU, C LOPESVIRELLA, MF AF VIRELLA, G MUNOZ, JF GALBRAITH, GMP GISSINGER, C CHASSEREAU, C LOPESVIRELLA, MF TI ACTIVATION OF HUMAN MONOCYTE-DERIVED MACROPHAGES BY IMMUNE-COMPLEXES CONTAINING LOW-DENSITY-LIPOPROTEIN SO CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article ID TUMOR-NECROSIS-FACTOR; SMOOTH-MUSCLE CELLS; HUMAN-ENDOTHELIAL-CELLS; HUMAN MONONUCLEAR-CELLS; POLYMORPHONUCLEAR LEUKOCYTES; VASCULAR ENDOTHELIUM; FATTY STREAKS; AORTIC INTIMA; FACTOR-ALPHA; TNF-ALPHA AB Human monocyte-derived macrophages are transformed into foam cells upon incubation with immune complexes containing low-density lipoprotein (LDL-IC), which are internalized predominantly through Fc gamma receptor-mediated phagocytosis. We investigated whether the FcR gamma-mediated ingestion of LDL-IC is associated with functional and metabolic activation of the ingesting cells. As end points we used the assay of released interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) and the reduction of nitroblue tetrazolium, which measures the respiratory burst. LDL-IC, added to the macrophages in concentrations known to induce intracellular accumulation of cholesterol esters and foam cell transformation, stimulated both the cytokine release and the respiratory burst more efficiently than control immune complexes. Time course studies of cytokine release and mRNA expression suggest that the synthesis and release of these two cytokines is under independent control. TNF alpha was released almost immediately after addition of LDL-IC to the macrophages, coinciding with increased early expression of TNF alpha mRNA, detectable 30 min after stimulation. In contrast, IL-1 beta was only increased in stimulated cell supernatants after 8 hr, and the onset of expression of IL-1 beta mRNA was also delayed in comparison to that of TNF alpha mRNA. We noted wide variations in the amounts of TNF alpha released by monocyte-derived macrophages from different donors. We also found that those macrophages which released higher levels of TNF alpha also took up higher amounts of I-125-labeled LDL, suggesting that the expression of LDL receptors by LDL-IC-stimulated macrophages is somehow linked to the degree of activation of these cells. Experiments using the measurement of the oxidative burst as end point corroborated that LDL-IC cause a general activation of macrophage functions. In conclusion, human macrophages are efficiently activated by LDL-IC, as reflected by the release of IL-1 beta and TNF alpha and by the release of oxygen active radicals. Thus, the presentation of LDL-IC to human macrophages induces a variety of metabolic and functional changes which are likely to contribute, directly or indirectly, to endothelial damage and progression of the atheromatous lesion. (C) 1995 Academic Press, Inc. C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. UNIV VIENNA,DEPT MED,VIENNA,AUSTRIA. RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC 29425. RP VIRELLA, G (reprint author), MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,CHARLESTON,SC 29425, USA. FU NHLBI NIH HHS [HL46815] NR 69 TC 55 Z9 60 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0090-1229 J9 CLIN IMMUNOL IMMUNOP JI Clin. Immunol. Immunopathol. PD MAY PY 1995 VL 75 IS 2 BP 179 EP 189 DI 10.1006/clin.1995.1069 PG 11 WC Immunology; Pathology SC Immunology; Pathology GA QT379 UT WOS:A1995QT37900012 PM 7704977 ER PT J AU CRAIG, WA AF CRAIG, WA TI INTERRELATIONSHIP BETWEEN PHARMACOKINETICS AND PHARMACODYNAMICS IN DETERMINING DOSAGE REGIMENS FOR BROAD-SPECTRUM CEPHALOSPORINS SO DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE LA English DT Article ID GRAM-NEGATIVE BACTEREMIA; THIGH-INFECTION; TISSUE-LEVELS; ANTIBIOTICS; CEFOTAXIME AB The broad-spectrum cephalosporins exhibit time-dependent bactericidal activity and produce prolonged postantibiotic effects only with staphylococci. The duration of time that serum levels exceed the minimum inhibitory concentration (MIG) is the important pharmacodynamic parameter correlating with efficacy for these drugs. Maximal efficacy for cephalosporins in several animal infection models is approached when serum levels are above the MIC for 60%-70% of the dosing interval for Enterobacteriaceae and streptococci and for 40%-50% of the dosing interval for Staphylococcus aureus. Based on MIC(90) values of 0.5 mu g/ml for enteric bacilli and 4 mu g/ml for S. aureus, these time above MIC goals can be easily met in infected and/or elderly patients following 1-2 g of cefotaxime at 12-h intervals. Full knowledge of the interrelationships between pharmacokinetics and pharmacodynamics is important for determining effective dosage regimens for the broad-spectrum cephalosporins. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR, DEPT MED, MADISON, WI USA. UNIV WISCONSIN, DEPT MED, MADISON, WI USA. NR 31 TC 390 Z9 410 U1 1 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0732-8893 EI 1879-0070 J9 DIAGN MICR INFEC DIS JI Diagn. Microbiol. Infect. Dis. PD MAY-JUN PY 1995 VL 22 IS 1-2 BP 89 EP 96 DI 10.1016/0732-8893(95)00053-D PG 8 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA RL195 UT WOS:A1995RL19500012 PM 7587056 ER PT J AU PAHLAVANI, MA HARRIS, MD MOORE, SA WEINDRUCH, R RICHARDSON, A AF PAHLAVANI, MA HARRIS, MD MOORE, SA WEINDRUCH, R RICHARDSON, A TI THE EXPRESSION OF HEAT-SHOCK PROTEIN-70 DECREASES WITH AGE IN LYMPHOCYTES FROM RATS AND RHESUS-MONKEYS SO EXPERIMENTAL CELL RESEARCH LA English DT Article ID GENE-EXPRESSION; TRANSCRIPTIONAL ACTIVATION; DIETARY RESTRICTION; MITOGEN STIMULATION; DIPLOID FIBROBLASTS; IMMUNE FUNCTION; MESSENGER-RNA; T-CELLS; STRESS; HSP70 AB The ability of cells to express heat shock proteins in response to a stress such as heat is universal to all organisms and is believed to play a critical protective role. Therefore, it was of interest to determine the influence of aging on the ability of lymphocytes to express the heat shock protein hsp70 in response to a heat shock (42.5 degrees C for 1 h). Splenic lymphocytes isolated from old (24-26 months) rats showed a marked decrease in the induction of hsp70 protein levels or hsp70 synthesis when compared to lymphocytes isolated from young (4-5 months) rats. An age-related decrease in the induction of hsp70 levels by heat also was observed in peripheral lymphocytes isolated from rhesus monkeys, The decline with age in the induction of hsp70 by lymphocytes from rats was paralleled by a decrease in the induction of hsp70 mRNA and the nuclear transcription of hsp70. In addition, it was found that the ability of extracts from heat-shocked lymphocytes to bind the heat shock element (HSE) decreased approximately 50% with age. Therefore, it appears that the reduced ability of lymphocytes from old rats to express hsp70 in response to a heat shock occurs at the level of transcription because of an alteration in the ability of the heat shock transcription factor to bind the HSE on the promoter of the hsp70 gene. The age-related decrease in the induction of hsp70 appears to be physiologically important because the viability of spleen lymphocytes exposed to high temperatures decreases significantly with age. (C) 1995 Academic Press, Inc. C1 AUDIE L MURPHY MEM VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN 182, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT MED, SAN ANTONIO, TX 78284 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN, MADISON, WI 53705 USA. UNIV WISCONSIN, DEPT MED, MADISON, WI 53705 USA. FU NIA NIH HHS [AG00165, AG00205, AG01548] NR 43 TC 49 Z9 49 U1 0 U2 0 PU ELSEVIER INC PI SAN DIEGO PA 525 B STREET, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4827 EI 1090-2422 J9 EXP CELL RES JI Exp. Cell Res. PD MAY PY 1995 VL 218 IS 1 BP 310 EP 318 DI 10.1006/excr.1995.1160 PG 9 WC Oncology; Cell Biology SC Oncology; Cell Biology GA QW998 UT WOS:A1995QW99800037 PM 7737368 ER PT J AU MOSER, AJ ABEDIN, MZ GIURGIU, DIN ROSLYN, JJ AF MOSER, AJ ABEDIN, MZ GIURGIU, DIN ROSLYN, JJ TI OCTREOTIDE PROMOTES GALLBLADDER ABSORPTION IN PRAIRIE DOGS - A POTENTIAL CAUSE OF GALLSTONES SO GASTROENTEROLOGY LA English DT Article ID LONG-TERM TREATMENT; SOMATOSTATIN ANALOG; BILIARY CALCIUM; ION-TRANSPORT; CYCLIC-AMP; CHOLESTEROL; SECRETION; BILE; SMS-201-995; NUCLEATION AB Background/Aims: Gallstone formation during octreotide administration has been causally linked to increased biliary concentrations of calcium, protein, and total lipids, all purported prolithogenic factors. These changes may be caused by octreotide-induced gallbladder stasis or a direct effect of octreotide on gallbladder absorption. We tested the hypothesis that octreotide stimulates gallbladder ion and water transport. Methods: Prairie dog gallbladders were mounted in Ussing chambers and bathed in oxygenated Ringer's solution. Electrophysiological parameters were recorded, and unidirectional Na+, Cl-, and H2O fluxes were measured before and after serosal exposure to 50 nmol/L octreotide. Results: Octreotide exposure caused a significant decrease in transepithelial short-circuit current and potential difference and an increase in tissue resistance compared with baseline. These alterations in electrophysiological parameters coincided with changes in ion transport. Octreotide stimulated net Na+ and H2O absorption and converted the gallbladder from a state of Cl- secretion to one of Cl- absorption by increasing mucosal to serosal fluxes. Octreotide effects on ion transport were blocked by 4,4'-diisothiocynostilbene-2,2'-disulfonic acid and amiloride and reversed by theophylline. Conclusions: Octreotide may promote gallstone formation by inducing gallbladder stasis and by directly increasing gallbladder absorption, which may act synergistically to increase the concentration of prolithogenic factors in bile and to facilitate nucleation and stone growth. C1 MED COLL PENN,DEPT SURG,PHILADELPHIA,PA 19129. UNIV CALIF LOS ANGELES,SCH MED,DEPT SURG,LOS ANGELES,CA 90024. VET AFFAIRS MED CTR,RES SERV,SEPULVEDA,CA. HAHNEMANN UNIV,PHILADELPHIA,PA 19102. VET AFFAIRS MED CTR,PHILADELPHIA,PA. NR 36 TC 11 Z9 11 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD MAY PY 1995 VL 108 IS 5 BP 1547 EP 1555 DI 10.1016/0016-5085(95)90705-X PG 9 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QV545 UT WOS:A1995QV54500027 PM 7729647 ER PT J AU KERN, RS GREEN, MF GOLDSTEIN, MJ AF KERN, RS GREEN, MF GOLDSTEIN, MJ TI MODIFICATION OF PERFORMANCE ON THE SPAN OF APPREHENSION, A PUTATIVE MARKER OF VULNERABILITY TO SCHIZOPHRENIA SO JOURNAL OF ABNORMAL PSYCHOLOGY LA English DT Note ID CARD SORTING TEST; CHILDREN; REMEDIATION; DEFICITS AB The present study examined the effectiveness of 2 types of intervention (contingent monetary reinforcement and enhanced instruction) on span of apprehension performance. Forty chronic schizophrenia inpatients (26 men, 14 women) received a 3- and 12-letter array version of the span of apprehension task 4 times: baseline, intervention, immediate posttest, and 1-week follow-up. All patients were randomly assigned to 1 of 4 groups that differed according to method of intervention: repeat administration, monetary reinforcement only, instruction only, and monetary reinforcement plus instruction. The combination of monetary reinforcement plus instruction yielded significantly greater improvement in span accuracy than the other methods of intervention. These findings suggest that performance on this putative vulnerability indicator can be modified through certain interventions. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHOL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. FU NIMH NIH HHS [MH 14584, MH 43292] NR 24 TC 47 Z9 48 U1 0 U2 0 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0021-843X J9 J ABNORM PSYCHOL JI J. Abnorm. Psychol. PD MAY PY 1995 VL 104 IS 2 BP 385 EP 389 DI 10.1037//0021-843X.104.2.385 PG 5 WC Psychology, Clinical; Psychology, Multidisciplinary SC Psychology GA QW681 UT WOS:A1995QW68100015 PM 7790641 ER PT J AU ATKINSON, BA BOUTHET, C BOCANEGRA, R CORREA, A LUTHER, MF GRAYBILL, JR AF ATKINSON, BA BOUTHET, C BOCANEGRA, R CORREA, A LUTHER, MF GRAYBILL, JR TI COMPARISON OF FLUCONAZOLE, AMPHOTERICIN-B AND FLUCYTOSINE IN TREATMENT OF A MURINE MODEL OF DISSEMINATED INFECTION WITH CANDIDA-GLABRATA IN IMMUNOCOMPROMISED MICE SO JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY LA English DT Article ID TORULOPSIS-GLABRATA; RESISTANCE; EPIDEMIOLOGY; YEASTS AB Candida glabrata is an emerging opportunist pathogen in immunosuppressed patients. C. glabrata is resistant to many antifungal agents and until recently, there have been no standard treatment regimens for this organism. A mouse model was established using mice immunosuppressed with 5 fluorouracil to evaluate amphotericin B, flucytosine, fluconazole and their combinations to treat an intravenously induced C. glabrata infection. Treatment with fluconazole, flucytosine, amphotericin B or a combination was begun one day after infection. Following 5 days of treatment, the mice were killed for fungal counts in kidneys and spleen. At the doses used, amphotericin B was superior to fluconazole or flucytosine alone in the treatment of C. glabrata infections. Flucytosine reduced the fungal burden in the kidney for only two of four isolates of C. glabrata. The combination of fluconazole and flucytosine was superior to these agents alone in reducing the tissue burden in the kidney for one isolate of C. glabrata. High doses of fluconazole alone produced modest reductions in kidney counts but did not reduce spleen tissue counts. There was poor correlation between in-vitro MICs and in-vivo results. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MICROBIOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 23 TC 22 Z9 22 U1 0 U2 0 PU W B SAUNDERS CO LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0305-7453 J9 J ANTIMICROB CHEMOTH JI J. Antimicrob. Chemother. PD MAY PY 1995 VL 35 IS 5 BP 631 EP 640 DI 10.1093/jac/35.5.631 PG 10 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA RD066 UT WOS:A1995RD06600009 PM 7592176 ER PT J AU BADR, MS TOIBER, F SKATRUD, JB DEMPSEY, J AF BADR, MS TOIBER, F SKATRUD, JB DEMPSEY, J TI PHARYNGEAL NARROWING OCCLUSION DURING CENTRAL SLEEP-APNEA SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE UPPER AIRWAY OBSTRUCTION; NON RAPID EYE MOVEMENT SLEEP; HYPOCAPNIA; PHARYNGEAL AIRWAY; PATHOGENESIS OF SLEEP APNEA SYNDROME ID UPPER AIRWAY PATENCY; NREM SLEEP; PULMONARY RESISTANCE; HUMAN INFANT; PRESSURE; PATHOGENESIS; WAKEFULNESS; OBSTRUCTION; VENTILATION; MECHANISM AB We hypothesized that subatmospheric intraluminal pressure is not required for pharyngeal occlusion during sleep. Six normal subjects and six subjects with sleep apnea or hypopnea (SAH) were studied during non-rapid-eye-movement sleep. Pharyngeal patency was determined by using fiber-optic nasopharyngoscopy during spontaneous central sleep apnea (n = 4) and induced hypocapnic central apnea via nasal mechanical ventilation (n = 10). Complete pharyngeal occlusion occurred in 146 of 160 spontaneously occurring central apneas in patients with central sleep apnea syndrome. During induced hypocapnic central apnea, gradual progressive pharyngeal narrowing occurred. More pronounced narrowing was noted at the velopharynx relative to the oropharynx and in subjects with SAH relative to normals. Complete pharyngeal occlusion frequently occurred in subjects with SAH (31 of 44 apneas) but rarely occurred in normals (3 of 25 apneas). Resumption of inspiratory effort was associated with persistent narrowing or complete occlusion unless electroencephalogram signs of arousal were noted. Thus pharyngeal cross-sectional area is reduced during central apnea in the absence of inspiratory effort. Velopharyngeal narrowing consistently occurs during induced hypocapnic central apnea even in normal subjects. Complete pharyngeal occlusion occurs during spontaneous or induced central apnea in patients with SAH. We conclude that subatmospheric intraluminal pressure is not required for pharyngeal occlusion to occur. Pharyngeal narrowing or occlusion during central apnea may be due to passive collapse or active constriction. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MED SERV,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT MED,JOHN RANKIN LAB PREVENT MED,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,JOHN RANKIN LAB PREVENT MED,MADISON,WI 53705. FU NCRR NIH HHS [RR-03186]; NHLBI NIH HHS [HL-02588, HL-42242] NR 41 TC 172 Z9 175 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD MAY PY 1995 VL 78 IS 5 BP 1806 EP 1815 PG 10 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA QY605 UT WOS:A1995QY60500027 PM 7649916 ER PT J AU FORTENBERRY, SC SCHOREY, JS CHIRGWIN, JM AF FORTENBERRY, SC SCHOREY, JS CHIRGWIN, JM TI ROLE OF GLYCOSYLATION IN THE EXPRESSION OF HUMAN PROCATHEPSIN-D SO JOURNAL OF CELL SCIENCE LA English DT Article DE PROCATHEPSIN D; PROTEIN GLYCOSYLATION; LYSOSOMAL TARGETING ID CATHEPSIN-D; LYSOSOMAL-ENZYMES; MOLECULAR-CLONING; SEQUENCE-ANALYSIS; HUMAN RENIN; CELLS; SECRETION; PROTEIN; EVOLUTION; PROTEASES AB Human procathepsin D carries two N-linked glycosylation sites at asparagine residues 70 and 199, widely separated on the surface of the folded protein. We created monoglycosylated procathepsin D molecules by site-directed mutagenesis in vitro of the individual glycosylation sites. With only two exceptions, all 12 mutants of this type were expressed efficiently in mammalian cells. The expressed proteins were stable, targeted to the lysosome, and partially secreted into the medium. When both glycosylation sites were eliminated, however, the expressed proteins (9 different mutants) were stable but most were not secreted and targeted poorly to the lysosome. Mammalian fibroblasts appear to sort nascent procathepsin D efficiently only if it is N-glycosylated. Procathepsin D monoglycosylated at N70 is readily distinguished from the endogenous protein in transfected human cells and thus provides an excellent substrate for studying lysosomal targeting in an homologous system. C1 UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL & METAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV,SAN ANTONIO,TX 78284. NR 35 TC 18 Z9 21 U1 1 U2 2 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0021-9533 J9 J CELL SCI JI J. Cell Sci. PD MAY PY 1995 VL 108 BP 2001 EP 2006 PN 5 PG 6 WC Cell Biology SC Cell Biology GA QY172 UT WOS:A1995QY17200017 PM 7657720 ER PT J AU SCHOREY, JS FORTENBERRY, SC CHIRGWIN, JM AF SCHOREY, JS FORTENBERRY, SC CHIRGWIN, JM TI LYSINE RESIDUES IN THE C-TERMINAL LOBE AND LYSOSOMAL TARGETING OF PROCATHEPSIN-D SO JOURNAL OF CELL SCIENCE LA English DT Article DE PROCATHEPSIN D; LYSOSOMAL TARGETING; PROTEIN SORTING ID HUMAN CATHEPSIN-D; PROTEIN RECOGNITION DETERMINANTS; HAMSTER KIDNEY-CELLS; BREAST-CANCER CELLS; ENZYME PHOSPHORYLATION; CARBOXYPEPTIDASE-Y; OLIGOSACCHARIDES; BIOSYNTHESIS; EXPRESSION; PROPEPTIDE AB A major pathway to the lysosome for soluble hydrolases involves the 6-phosphorylation of mannose residues. The initial step in this reaction is catalyzed by a phosphotransferase which recognizes lysosomal precursors. We constructed mutants of human procathepsin D whose targeting to the lysosome could be assayed directly in intact cells. Eight lysine residues were individually converted to glutamic acid on the surface of the carboxyl terminal lobe of the protein. Mutants with as many as four Lys to Glu mutations were normally targeted to the lysosome and processed to the mature form of the enzyme in transfected cells. We conclude that the C-terminal lobe of procathepsin D may not carry a determinant essential for lysosomal targeting in intact fibroblasts. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV ENDOCRINOL & METAB,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT BIOCHEM,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV,SAN ANTONIO,TX 78284. FU NCI NIH HHS [CA40035] NR 60 TC 12 Z9 12 U1 0 U2 0 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0021-9533 J9 J CELL SCI JI J. Cell Sci. PD MAY PY 1995 VL 108 BP 2007 EP 2015 PN 5 PG 9 WC Cell Biology SC Cell Biology GA QY172 UT WOS:A1995QY17200018 PM 7657721 ER PT J AU HERSHMAN, JM AF HERSHMAN, JM TI DOES THYROXINE THERAPY PREVENT RECURRENCE OF GRAVES HYPERTHYROIDISM SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Editorial Material ID MEDICAL-TREATMENT; DISEASE; RISK RP HERSHMAN, JM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, 11301 WILSHIRE BLVD, LOS ANGELES, CA 90073 USA. NR 13 TC 8 Z9 8 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD MAY PY 1995 VL 80 IS 5 BP 1479 EP 1480 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QW611 UT WOS:A1995QW61100002 PM 7744988 ER PT J AU YIP, I PANG, XP BERG, L HERSHMAN, JM AF YIP, I PANG, XP BERG, L HERSHMAN, JM TI ANTITUMOR ACTIONS OF INTERFERON-GAMMA AND INTERLEUKIN-1-BETA ON HUMAN PAPILLARY THYROID-CARCINOMA CELL-LINES SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID TUMOR-NECROSIS-FACTOR; RECONSTITUTED BASEMENT-MEMBRANE; FACTOR-ALPHA; PROGNOSTIC FACTORS; RECOMBINANT ALPHA-2-INTERFERON; IMMUNE INTERFERON; INVITRO ASSAY; CANCER; INVASION; INHIBITION AB To test the hypothesis that interferon-gamma (IFN gamma) and interleukin-1 beta (IL-1 beta) possess antitumor activity on human papillary thyroid carcinoma cells, we studied the in vitro effects of IFN gamma and IL-1 beta on the proliferation and invasiveness of two human PTC cell lines, TPC-1 (TP) and NPA (NP) cells. TP and NP cells were treated with various concentrations ofIFN gamma and IL-1 beta alone and in combination. Cell proliferation was assessed by [H-3]thymidine incorporation and cell number measurement. Tumor cell invasion was assessed by the ability of cells to penetrate through a Matrigel membrane. Both IFN gamma and IL-1 beta inhibited [H-3]thymidine incorporation into TP cells in a dose-dependent manner and decreased TP cell number. In NP cells, treatment with IFN gamma and IL-1 beta also decreased [H-3]thymidine incorporation and cell number. The inhibitory effects of IFN gamma and IL-1 beta on tumor cell proliferation were additive in both cell lines. In the invasion experiments, IFN gamma and IL-1 beta reduced the invasiveness of TP and NP cells. Again, the inhibitory effects of IFN gamma and IL-1 beta on tumor cell invasion were additive in both cell lines. In summary, the results showed that both IFN gamma and IL-1 beta are potent inhibitors of the proliferation and invasiveness of TP and NP cells. The additive effects of IFN gamma and IL-1 beta are evidence that they act through different pathways. Our findings suggest that IFN gamma and IL-1 beta are two of the anticancer factors that act to suppress the proliferation and reduce the invasive potential of human papillary thyroid carcinoma cells. C1 W LOS ANGELES VET AFFAIRS MED CTR, ENDOCRINE RES LAB, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90073 USA. FU NCI NIH HHS [CA-61863] NR 48 TC 30 Z9 31 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD MAY PY 1995 VL 80 IS 5 BP 1664 EP 1669 DI 10.1210/jc.80.5.1664 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QW611 UT WOS:A1995QW61100035 PM 7745015 ER PT J AU PFALLER, MA BALE, M BUSCHELMAN, B LANCASTER, M ESPINELINGROFF, A REX, JH RINALDI, MG COOPER, CR MCGINNIS, MR AF PFALLER, MA BALE, M BUSCHELMAN, B LANCASTER, M ESPINELINGROFF, A REX, JH RINALDI, MG COOPER, CR MCGINNIS, MR TI QUALITY-CONTROL GUIDELINES FOR NATIONAL COMMITTEE FOR CLINICAL LABORATORY STANDARDS RECOMMENDED BROTH MACRODILUTION TESTING OF AMPHOTERICIN-B, FLUCONAZOLE, AND FLUCYTOSINE SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SUSCEPTIBILITY TESTS AB Amphotericin B, fluconazole, and flucytosine (5FC) were tested in a multilaboratory study to establish quality control (QC) guidelines far yeast antifungal susceptibility testing. Ten candidate QC strains were tested in accordance with National Committee for Clinical Laboratory Standards M27-P guidelines against the three antifungal agents in each of six laboratories. Each laboratory was assigned a unique lot of RPMI 1640 broth medium as well as a lot of RPMI 1640 common to all of the laboratories. The candidate QC strains were tested 20 times each against the three antifungal agents in bath unique and common lots of RPMI 1640. A minimum of 220 MICs per drug per organism were generated during the study. Overall, 95% of the MICs of amphotericin B, fluconazole, and 5FC fell within the desired 3 log(2)-dilution range (mode +/- 1 log(2) dilution), Excellent performance with all three drugs was observed for Candida parapsilosis ATCC 22019 and C. krusei ATCC 6258. With these strains, an-scale 3 log(2)-dilution ranges encompassing 96 to 99% of the MICs of all three drugs were established. These two strains are recommended for QC testing of amphotericin B, fluconazole, and 5FC. Reference ranges were also established for an additional four strains for use in method development and for training. Four strains failed to perform adequately for recommendation as either QC or reference strains. C1 ALAMAR BIOSCI INC,SACRAMENTO,CA 95834. VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA,RICHMOND,VA 23298. UNIV TEXAS,SCH MED,HOUSTON,TX 77030. UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,LAB SERV,SAN ANTONIO,TX 78284. UNIV TEXAS,MED BRANCH,GALVESTON,TX 77555. RP PFALLER, MA (reprint author), UNIV IOWA,COLL MED,IOWA CITY,IA 52242, USA. NR 14 TC 106 Z9 108 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAY PY 1995 VL 33 IS 5 BP 1104 EP 1107 PG 4 WC Microbiology SC Microbiology GA QT306 UT WOS:A1995QT30600012 PM 7615713 ER PT J AU LEONG, GB SILVA, JA AF LEONG, GB SILVA, JA TI A PSYCHIATRIC-LEGAL ANALYSIS OF PSYCHOTIC CRIMINAL DEFENDANTS CHARGED WITH MURDER SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE PSYCHIATRY; PSYCHOSIS; HOMICIDE; MURDER; MENTAL DISORDER; VIOLENCE; AGGRESSION AB A sample of 25 criminal defendants charged with murder and suffering from psychosis from a large urban multi-ethnic, multi-cultural community pool was studied. Subject characteristics and information about the homicide and decedents are described. Suggestions for further study are briefly discussed. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP LEONG, GB (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV 116AA,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 19 TC 10 Z9 10 U1 2 U2 3 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD MAY PY 1995 VL 40 IS 3 BP 445 EP 448 PG 4 WC Medicine, Legal SC Legal Medicine GA RB239 UT WOS:A1995RB23900017 PM 7782749 ER PT J AU HE, HD MCKAY, G WIRSHING, B MIDHA, KK AF HE, HD MCKAY, G WIRSHING, B MIDHA, KK TI DEVELOPMENT AND APPLICATION OF A SPECIFIC AND SENSITIVE RADIOIMMUNOASSAY FOR TRIHEXYPHENIDYL TO A PHARMACOKINETIC STUDY IN HUMANS SO JOURNAL OF PHARMACEUTICAL SCIENCES LA English DT Article ID CHROMATOGRAPHIC METHOD; PARKINSONS-DISEASE; GAS-CHROMATOGRAPHY; BENZHEXOL; PLASMA; HYDROCHLORIDE; QUANTITATION; MEMORY AB A radioimmunoassay (RIA) for trihexyphenidyl was developed through the use of a bovine thyroglobulin conjugate of trihexyphenidyl hemisuccinate. Immunization of New Zealand white rabbits with this drug-protein conjugate yielded antisera, for which the antibody titer and specificity were evaluated. An antiserum that had the highest titer and minimal cross-reactivities to major metabolites of trihexyphenidyl, such as trihexyphenidyl N-oxide (2%), hydroxytrihexyphenidyl (1%), and the antipsychotic drugs fluphenazine (<1%), flupenthixol (<1%), chlorpromazine (<1%), and haloperidol (<1%), was selected for development of a RIA. The described RIA enables the quantitation of 7.8 pg of trihexyphenidyl in 200 mu L of human plasma with a mean coefficient of variation of <6% across the range of the standard curve. Assay specificity was further demonstrated by comparison of results obtained directly and after selective extraction of trihexyphenidyl from replicate samples. This RIA procedure was applied to the analysis of steady state plasma samples obtained from patients undergoing treatment with trihexyphenidyl (2-8 mg) and plasma samples obtained from eight healthy male volunteers after administration of a single 4 mg oral dose of the drug. The results of the latter single dose studies demonstrated that the mean +/-SD for the peak concentration (C-max), the time to C-max (T-max), the rate of absorption (K-a), and the area under the curve from 0 to 72 h (AUC(0-72)) were found to be 7.15 +/- 2.58 ng/mL, 1.32 +/- 0.58 h, 2.07 +/- 0.93 1/h, and 201 +/- 71 ng h/mL, respectively. The human subjects had a biphasic plot of mean plasma concentration Versus time consisting of an initially rapid distribution phase (T-alpha 1/2 = 5.33 +/- 3.23 h) and a later slower elimination phase (T-beta 1/2 = 32.7 +/- 6.35 h). C1 UNIV SASKATCHEWAN,COLL PHARM & NUTR,SASKATOON,SK S7N 0W0,CANADA. UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. NR 29 TC 6 Z9 6 U1 0 U2 1 PU AMER PHARMACEUTICAL ASSN PI WASHINGTON PA 2215 CONSTITUTION AVE NW, WASHINGTON, DC 20037 SN 0022-3549 J9 J PHARM SCI JI J. Pharm. Sci. PD MAY PY 1995 VL 84 IS 5 BP 561 EP 567 DI 10.1002/jps.2600840509 PG 7 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA QX151 UT WOS:A1995QX15100008 PM 7658345 ER PT J AU GARITI, P ALTERMAN, AI HOLUBBEYER, E VOLPICELLI, JR PRENTICE, N OBRIEN, CP AF GARITI, P ALTERMAN, AI HOLUBBEYER, E VOLPICELLI, JR PRENTICE, N OBRIEN, CP TI EFFECTS OF AN APPOINTMENT REMINDER CALL ON PATIENT SHOW RATES SO JOURNAL OF SUBSTANCE ABUSE TREATMENT LA English DT Article DE WAITING LISTS; DROPOUT; TREATMENT RETENTION; INITIAL APPOINTMENTS; SHOW RATES; SUBSTANCE ABUSERS ID ABUSE TREATMENT; WAITING LIST; RETENTION; DISORDERS AB A pilot study (N = 80) was conducted to determine if (I) prospective substance-dependent patients randomly selected to be reminded (TC) of their scheduled intake evaluation the day before their first appointment would have a higher show rate than those not contacted (NC) and (2) if TC subjects administered a satisfaction questionnaire 1-3 days after intake would exhibit higher treatment retention rates at one week and one month posttreatment entry than NC subjects not exposed to the questionnaire. The findings suggest that reminding prospective patients of their initial scheduled appointments and following up with phone calls to those who fail to show can improve the rate at which patients will initiate treatment, provided initial appointments are scheduled in a timely manner (7 days or less). Similarly, the combination of the reminder call and the satisfaction questionnaire were associated with higher treatment retention rates for those whose initial appointments were scheduled in a timely manner. C1 UNIV PENN,SCH MED,DIV ADDICT STUDIES,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. FU NIDA NIH HHS [DA05186] NR 25 TC 28 Z9 28 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0740-5472 J9 J SUBST ABUSE TREAT JI J. Subst. Abus. Treat. PD MAY-JUN PY 1995 VL 12 IS 3 BP 207 EP 212 DI 10.1016/0740-5472(95)00019-2 PG 6 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA RP285 UT WOS:A1995RP28500006 PM 7474028 ER PT J AU IMAMURA, A MACKENZIE, HS LACY, ER HUTCHISON, FN FITZGIBBON, WR PLOTH, DW AF IMAMURA, A MACKENZIE, HS LACY, ER HUTCHISON, FN FITZGIBBON, WR PLOTH, DW TI EFFECTS OF CHRONIC TREATMENT WITH ANGIOTENSIN-CONVERTING ENZYME-INHIBITOR OR AN ANGIOTENSIN RECEPTOR ANTAGONIST IN 2-KIDNEY, ONE-CLIP HYPERTENSIVE RATS SO KIDNEY INTERNATIONAL LA English DT Article ID ACTIVE ANTIHYPERTENSIVE AGENT; BILATERAL RENAL-FUNCTION; RENOVASCULAR HYPERTENSION; INDUCED PROTEINURIA; 2-KIDNEY; RESPONSES; BLOCKADE; THERAPY; DUP-753; INJURY AB The effects of chronic angiotensin II (Ang II) receptor blockade (losartan) or converting enzyme inhibition (enalapril) on blood pressure (BP), urinary albumin excretion (UalbV), renal histology and the hemodynamic and excretory function of the clipped and nonclipped kidneys were studied in two-kidney, one-clip (2-K 1-C) rats. One day after clipping the right renal artery, male Wistar rats were divided into three groups receiving: (1) losartan, 20 mg/kg/day (N = 7), (2) enalapril, 20 mg/kg/day (N = 8), or (3) no treatment (controls, N = 9) for three weeks. Both losartan and enalapril treatments maintained conscious BP at comparably lowered levels compared to control animals (116 +/- 6 mm Hg and 113 +/- 2 mm Hg vs. 188 +/- 11 mm Hg, respectively, P < 0.01). Treatment also prevented the increase in UalbV, observed for the untreated group, three weeks after clipping (1.7 +/- 0.5 and 0.7 +/- 0.1 mg/24 hr vs. 17.5 +/- 7 mg/24 hr, respectively, P < 0.01). After three weeks of treatment, acute study of renal function during pentobarbital anesthesia revealed higher values of GFR and RPF and lowered vascular resistance for nonclipped kidneys from the losartan and enalapril groups compared to the corresponding kidneys from control animals. Despite the lower BP of both treated groups, clipped kidney GFR and RPF were unchanged compared to the control group. UalbV for nonclipped kidneys from untreated rats was approximately 5- to 10-fold higher than in the nonclipped kidneys from the treated groups. Histological evaluation revealed evidence of early glomerulosclerosis in the nonclipped kidneys from the untreated but not the treated groups, and decreased indices of glomerular size and mesangial expansion in clipped kidneys for the treated groups compared to the untreated group. These data support a primary role for Ang II modulation of blood pressure and renal function in the nonclipped kidney of the 2-K 1-C rat. C1 MED UNIV S CAROLINA,DEPT MED,DIV NEPHROL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT CELL BIOL & ANAT,CHARLESTON,SC 29425. RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC 29403. FU NIDDK NIH HHS [DK 43186] NR 33 TC 44 Z9 46 U1 0 U2 2 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD MAY PY 1995 VL 47 IS 5 BP 1394 EP 1402 DI 10.1038/ki.1995.196 PG 9 WC Urology & Nephrology SC Urology & Nephrology GA QV942 UT WOS:A1995QV94200020 PM 7637269 ER PT J AU CRAIG, WA ANDES, DR AF CRAIG, WA ANDES, DR TI PARENTERAL VERSUS ORAL ANTIBIOTIC-THERAPY SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Article ID RESPIRATORY-TRACT INFECTIONS; INTRAVENOUS CEFTAZIDIME; CHRONIC OSTEOMYELITIS; CYSTIC-FIBROSIS; SKIN STRUCTURE; CIPROFLOXACIN; EFFICACY; ENDOCARDITIS; CEFTRIAXONE; ARTHRITIS AB The past decade has seen increased use of oral therapy for a variety of infections previously treated exclusively by the parenteral route. Clinical trials exhibit similar efficacy between parenteral and oral or step-down therapy. Much of the success is because of the availability of new oral agents with enhanced activity against gram-negative bacilli and improved pharmacokinetics. Oral therapy can reduce the cost of antimicrobial therapy significantly. C1 UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,DEPT INTERNAL MED,MADISON,WI. RP CRAIG, WA (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT INFECT DIS,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 39 TC 38 Z9 38 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAY PY 1995 VL 79 IS 3 BP 497 EP 508 PG 12 WC Medicine, General & Internal SC General & Internal Medicine GA QX447 UT WOS:A1995QX44700004 PM 7752724 ER PT J AU MA, MY RHO, JP AF MA, MY RHO, JP TI CONSIDERATIONS IN ANTIMICROBIAL PRESCRIBING SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Article ID MEDICATION; THERAPY; FREQUENCY; AGENTS; IMPACT; TWICE AB Factors important to the clinician in prescribing an antimicrobial regimen include the selection of the most effective agent(s), avoidance of adverse drug reactions, and the ensurance of patient compliance. This article discusses various strategies that can be used by physicians and hospitals to improve cost-effective use of antimicrobial drugs. C1 UNIV CALIF SAN FRANCISCO,SCH PHARM,SAN FRANCISCO,CA. UNIV SO CALIF,SCH PHARM,LOS ANGELES,CA. RP MA, MY (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,PHARM SERV 119,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 29 TC 7 Z9 7 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAY PY 1995 VL 79 IS 3 BP 537 EP 549 PG 13 WC Medicine, General & Internal SC General & Internal Medicine GA QX447 UT WOS:A1995QX44700007 PM 7752727 ER PT J AU YOSHIKAWA, TT NORMAN, DC AF YOSHIKAWA, TT NORMAN, DC TI TREATMENT OF INFECTIONS IN ELDERLY PATIENTS SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Article ID COMMUNITY-ACQUIRED PNEUMONIA; NERVOUS-SYSTEM INFECTIONS; URINARY-TRACT INFECTIONS; BACTERIAL-MENINGITIS; REQUIRING HOSPITALIZATION; CONSECUTIVE EPISODES; OLMSTED COUNTY; DIAGNOSIS; EPIDEMIOLOGY; BACTEREMIA AB Infections are common in elderly persons. The clinical manifestations of infection may be atypical or absent in the elderly. The microbial cause for many common infections may be more diverse in elderly patients, and obtaining diagnostic clinical specimens often is more difficult. Aging is associated with changes in pharmacokinetics and a higher rate of adverse drug reactions. These factors impact on the approach to treating infections in the elderly. C1 GEORGE WASHINGTON UNIV,DEPT HLTH CARE SCI,WASHINGTON,DC. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA. RP YOSHIKAWA, TT (reprint author), US DEPT VET AFFAIRS,OFF GERIATR & EXTENDED CARE 114,810 VERMONT AVE NW,WASHINGTON,DC 20420, USA. NR 62 TC 22 Z9 22 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAY PY 1995 VL 79 IS 3 BP 651 EP 661 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA QX447 UT WOS:A1995QX44700013 PM 7752733 ER PT J AU RIEHMANN, M KNES, JM HEISEY, D MADSEN, PO BRUSKEWITZ, RC AF RIEHMANN, M KNES, JM HEISEY, D MADSEN, PO BRUSKEWITZ, RC TI TRANSURETHRAL RESECTION VERSUS INCISION OF THE PROSTATE - A RANDOMIZED, PROSPECTIVE-STUDY SO UROLOGY LA English DT Article ID TRANS-URETHRAL RESECTION; BLADDER NECK INCISION; CANCER AB Objectives, To evaluate longer term effects of transurethral resection (TURF) and incision (TUIP) of the prostate in randomized patients. Methods, In a randomized, prospective study, 120 patients with symptoms of bladder outlet obstruction caused by smaller benign prostates (estimated resectable weight less than 20 g) were assigned to TURF or TUIP, Patients were evaluated preoperatively and at intervals postoperatively as to urinary symptoms (Madsen's questionnaire), sexual function, and uroflowmetry. Overall evaluation of outcome of surgery was also assessed at follow-up visits. Results, Fifty-six patients received a TURF and 61 a TUIP, Three patients refused to participate in the project after randomization, and 5 patients were lost to or excluded from follow-up. A group of 1 12 patients were obtainable for postoperative evaluation with a mean follow-up time of 34 months (1 to 82 months). Improvements in mean urinary peak flow rates were seen in both groups throughout the study period. The peak flow rates generally were higher (but not statistically so) in the TURF group. Postoperative irritative, obstructive, as well as total symptom scores decreased significantly at all followup visits after both TURF and TUIP (P less than or equal to 0.034). Preoperatively and at all postoperative follow-up there was no statistically significant difference in irritative, obstructive, or total symptom scores between TURF and TUIP. The patients indicated an overall subjective improvement at all follow-ups in both groups, with no statistically significant difference be tween the treatment groups. Fifteen of 22 (68%) patients receiving TURF and 8 of 23 (35%) in the TUIP group who were sexually active before and after surgery developed postoperative retrograde ejaculation (P = 0.020), Postoperatively, 9 (16%) of the patients in the TURF and 14 (23%) in the TUIP group received further treatment for benign prostatic hyperplasia (BPH)-related infravesical obstruction. This difference was not statistically significant (P = 0.908). Conclusions. In small prostates TURF and TUIP were generally equally effective in relieving bladder outlet obstruction secondary to BPH. Most surgically treated BPH cases can be well managed by the incision technique, which is an underutilized procedure. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,DIV UROL,MADISON,WI. RP RIEHMANN, M (reprint author), UNIV WISCONSIN HOSP & CLIN,DEPT SURG,DIV UROL,MADISON,WI 53792, USA. NR 30 TC 58 Z9 61 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD MAY PY 1995 VL 45 IS 5 BP 768 EP 775 DI 10.1016/S0090-4295(99)80081-8 PG 8 WC Urology & Nephrology SC Urology & Nephrology GA QW467 UT WOS:A1995QW46700011 PM 7538238 ER PT J AU FORTENBERRY, SC CHIRGWIN, JM AF FORTENBERRY, SC CHIRGWIN, JM TI THE PROPEPTIDE IS NONESSENTIAL FOR THE EXPRESSION OF HUMAN CATHEPSIN-D SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID LYSOSOMAL-ENZYME PHOSPHORYLATION; PROTEIN RECOGNITION DETERMINANTS; HAMSTER KIDNEY-CELLS; ACTIVE HUMAN RENIN; PROCATHEPSIN-D; ASPARTIC PROTEINASES; PORCINE PEPSINOGEN; TARGETING SIGNAL; CRYSTAL-STRUCTURES; SITE AB When the 44-amino acid propeptide of human procathepsin D was deleted by mutagenesis in vitro, the mature protein was stably expressed and secreted from transfected mammalian cells. The secreted protein was correctly folded as judged by its binding to pepstatinyl-agarose. We were unable to detect lysosomal targeting of the propeptide-deleted protein, and targeting was not restored by the substitution of the propeptides from pepsin or renin. We conclude that its propeptide is not essential for the folding of nascent cathepsin D. Efficient lysosomal targeting in mammalian cells appears to require the precursor form of the molecule. C1 UNIV TEXAS, HLTH SCI CTR, DEPT MED, DIV ENDOCRINOL & METAB, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT BIOCHEM, SAN ANTONIO, TX 78284 USA. AUDIE L MURPHY MEM VET ADM MED CTR, RES SERV, SAN ANTONIO, TX 78284 USA. NR 61 TC 24 Z9 24 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD APR 28 PY 1995 VL 270 IS 17 BP 9778 EP 9782 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QV417 UT WOS:A1995QV41700015 PM 7730356 ER PT J AU LEWIS, BS AF LEWIS, BS TI EFFICACY AND SAFETY OF NISOLDIPINE COAT CORE IN THE MANAGEMENT OF ANGINA-PECTORIS, SYSTEMIC HYPERTENSION, AND ISCHEMIC VENTRICULAR DYSFUNCTION SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article; Proceedings Paper CT Symposium on Left Ventricular Dysfunction, Calcium Antagonism, and Nisoldipine CY JUN 23-26, 1994 CL AMSTERDAM, NETHERLANDS SP BAYER AG, LEVERKUSEN ID CONGESTIVE-HEART-FAILURE; CALCIUM-CHANNEL BLOCKER; MYOCARDIAL-INFARCTION; DOUBLE-BLIND; WALL-MOTION; NIFEDIPINE; THERAPY; ABNORMALITIES; ANTAGONISTS; HIBERNATION AB The effects of the long-acting dihydropyridine calcium antagonist nisoldipine coat core (CC) have been investigated in >3,500 patients with angina pectoris, hypertension, and ischemic ventricular dysfunction. in patients with angina pectoris, nisoldipine CC improved total treadmill exercise duration (p = 0.027), delayed the onset of angina pectoris (p = 0.009), and increased time to exercise-induced ST-segment depression (p = 0.061). In general, nisoldipine 20-40 mg was effective, and the dose-response curve flattened thereafter. in patients with hypertension, 10-40 mg once daily as monotherapy reduced blood pressure (p < 0.05), with a fall in diastolic pressure of greater than or equal to 10 mm Hg or a final diastolic pressure of <90 mm Hg in 35-63% of patients. In most patients followed for a year, nisoldipine CC was continued as monotherapy. Efficacy was similar in patients <65 and >65 years of age. In the Doppler Flow and Echocardiography in Functional Cardiac Insufficiency: Assessment of Nisoldipine Therapy (DEFIANT-I) study of patients recovering from myocardial infarction, nisoldipine CC had a salutary effect on diastolic ventricular function, with a higher transmitral early filling velocity and shorter isovolumic relaxation time than in patients receiving placebo. Bicycle exercise capacity was greater (by 12 W; 95% confidence interval, 0.8-23.3) and exercise-induced ischemia occurred less frequently, The nisoldipine CC data pool (3,679 patients) showed that the drug was well tolerated with a low incidence of side effects. Angina pectoris, chest pain, and dyspnea occurred less frequently with nisoldipine CC, and mortality was slightly lower in patients taking the drug (0.28% vs 0.37%). The absence or very low incidence of acute vasodilatory effects may indicate that complications associated with sharp peak and trough effects of drug (reflex sympathetic stimulation, catecholamine release, activation of the renin-angiotensin system) may be less frequent with long-acting calcium antagonists. C1 UNIV CALIF LOS ANGELES, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, DIV CARDIOL, LOS ANGELES, CA 90073 USA. NR 28 TC 18 Z9 18 U1 0 U2 0 PU EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC PI BRIDGEWATER PA 685 ROUTE 202-206 STE 3, BRIDGEWATER, NJ 08807 USA SN 0002-9149 EI 1879-1913 J9 AM J CARDIOL JI Am. J. Cardiol. PD APR 27 PY 1995 VL 75 IS 13 BP E46 EP E53 DI 10.1016/S0002-9149(99)80448-2 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QU980 UT WOS:A1995QU98000009 PM 7726125 ER PT J AU HARRIS, RA MCQUILKIN, SJ PAYLOR, R ABELIOVICH, A TONEGAWA, S WEHNER, JM AF HARRIS, RA MCQUILKIN, SJ PAYLOR, R ABELIOVICH, A TONEGAWA, S WEHNER, JM TI MUTANT MICE LACKING THE GAMMA-ISOFORM OF PROTEIN-KINASE-C SHOW DECREASED BEHAVIORAL ACTIONS OF ETHANOL AND ALTERED FUNCTION OF GAMMA-AMINOBUTYRATE TYPE-A RECEPTORS SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID SHORT-SLEEP MICE; CEREBELLAR PURKINJE NEURONS; RECOMBINANT INBRED STRAINS; ACID-INDUCED DEPRESSIONS; CENTRAL-NERVOUS-SYSTEM; GATED ION CHANNELS; LONG-SLEEP; CHLORIDE CHANNELS; GABA(A) RECEPTOR; A RECEPTOR AB Calcium/phospholipid-dependent protein kinase (protein kinase C, PKC) has been suggested to play a role in the sensitivity of gamma-aminobutyrate type A (GABA(A)) receptors to ethanol. We tested a line of null mutant mice that lacks the gamma isoform of PKC (PKC gamma) to determine the role of this brain-specific isoenzyme in ethanol sensitivity. We found that the mutation reduced the amount of PKC gamma immunoreactivity in cerebellum to undetectable levels without altering the levels of the alpha, beta(I), or beta(II) isoforms of PKC. The mutant mice display reduced sensitivity to the effects of ethanol on loss of righting reflex and hypothermia but show normal responses to flunitrazepam or pentobarbital. Likewise, GABA(A) receptor function of isolated,brain membranes showed that the mutation abolished the action of ethanol but did not alter actions of flunitrazepam or pentobarbital. These studies show the unique interactions of ethanol with GABA(A) receptors and suggest protein kinase isoenzymes as possible determinants of genetic differences in response to ethanol. C1 UNIV COLORADO, HLTH SCI CTR, DEPT PHARMACOL, DENVER, CO 80262 USA. UNIV COLORADO, INST BEHAV GENET, BOULDER, CO 80309 USA. UNIV COLORADO, SCH PHARM, BOULDER, CO 80309 USA. MIT, CTR CANC RES, CAMBRIDGE, MA 02139 USA. MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA. MIT, CTR CANC RES, HOWARD HUGHES MED INST, CAMBRIDGE, MA 02139 USA. MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA. RP UNIV COLORADO, HLTH SCI CTR, DENVER VET AFFAIRS MED CTR, 4200 E 9TH AVE, DENVER, CO 80262 USA. FU NIAAA NIH HHS [AA00141, AA03527, AA06399] NR 45 TC 212 Z9 215 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 25 PY 1995 VL 92 IS 9 BP 3658 EP 3662 DI 10.1073/pnas.92.9.3658 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA QV930 UT WOS:A1995QV93000005 PM 7731960 ER PT J AU KASINATH, BS AF KASINATH, BS TI EFFECT OF INSULIN ON HIGH-GLUCOSE MEDIUM-INDUCED CHANGES IN RAT GLOMERULAR EPITHELIAL-CELL METABOLISM OF GLYCOCONJUGATES SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE PROTEOGLYCANS; DIABETIC NEPHROPATHY; BASEMENT MEMBRANE; INSULIN ID HEPARAN-SULFATE PROTEOGLYCAN; STREPTOZOTOCIN DIABETIC RATS; BASEMENT-MEMBRANE; EXTRACELLULAR-MATRIX; INCREASED PERMEABILITY; REICHERTS MEMBRANE; GLYCOSAMINOGLYCANS; NEPHROPATHY; PREVENTION; COMPONENTS AB We have previously reported that incubation of rat glomerular epithelial cells in vitro for 8 days with 30 mM glucose without insulin results in reduction in the synthesis of a cell layer heparan sulfate proteoglycan (HSPG) species that has hydrodynamic size and antigenic characteristics of glomerular basement membrane HSPG (1994, Arch, Biochem, Biophys 309, 149-159). In these studies, reduction in HSPG synthesis could be attributed either to high-glucose medium or to insulin deficiency. In this study we investigated the effects of insulin replacement on changes in glomerular epithelial cell metabolism of glycoconjugates induced by high-glucose medium. Addition of pharmacologic concentrations of insulin prevented the following changes induced by 30 mM glucose: (a) increment in (SO4)-S-35 incorporation into macromolecules in cell layer and medium, (b) increment in the synthesis of low anionic macromolecules, probably glycoproteins, in both cell layer and medium, (c) increment in synthesis of small-sized glycosaminoglycans (K-av 0.75 on Sephrose CL-4B) associated with the cell layer. Insulin was unable to correct the 30 mM glucose-induced reduction in the synthesis of cell layer HSPG that resembles glomerular basement membrane HSPG. Physiologic concentrations of insulin did not affect any of the changes in glycopeptide metabolism induced by 30 mM glucose, These findings suggest that (a) inhibition of glomerular epithelial cell synthesis of (SO4)-S-35-labeled low anionic macromolecules, probably glycoproteins, may be involved in insulin-induced reversal of glomerular hypertrophy seen in early diabetes, and (b) mechanisms other than insulin lack are involved in reduction in the synthesis of glomerular basement membrane HSPG in diabetic nephropathy. (C) 1995 Academic Press, Inc. C1 UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. FU NIDDK NIH HHS [DK41517] NR 47 TC 13 Z9 14 U1 0 U2 1 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD APR 20 PY 1995 VL 318 IS 2 BP 286 EP 294 DI 10.1006/abbi.1995.1232 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QV624 UT WOS:A1995QV62400006 PM 7733656 ER PT J AU DUBINETT, SM HUANG, M DHANANI, S ECONOMOU, JS WANG, J LEE, P SHARMA, S DOUGHERTY, GJ MCBRIDE, WH AF DUBINETT, SM HUANG, M DHANANI, S ECONOMOU, JS WANG, J LEE, P SHARMA, S DOUGHERTY, GJ MCBRIDE, WH TI DOWN-REGULATION OF MURINE FIBROSARCOMA TRANSFORMING GROWTH-FACTOR-BETA-1 EXPRESSION BY INTERLEUKIN-7 SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID ACTIVATED KILLER-CELLS; HUMAN PERIPHERAL-BLOOD; FACTOR-BETA; ADOPTIVE IMMUNOTHERAPY; TUMOR REJECTION; LYMPHOCYTES-T; TGF-BETA; INHIBITION; CANCER; IL-7 AB Background: Cytokine genes encode proteins that modulate immune system responses, Modification of tumor cells by the introduction of cytokine genes has been used as a strategy to augment host immunity. Interleukin 7 (IL-7) gene transfer enhances the immune response to tumor cells and can result in tumor regression. Transforming growth factor-beta 1 (TGF-beta 1) is a potent immunosuppressive cytokine produced by many tumors. We have previously reported that recombinant IL-7 decreases the expression of TGF-beta 1 by murine macrophages. Purpose: This study investigates the inhibition of tumor-derived TGF-beta 1 production as a possible mechanism for the enhanced antitumor immunity that accompanies IL-7 gene transfer. Methods: A fibrosarcoma cell line (FSA-JmIL-7) genetically modified to produce IL-7 was used to evaluate the effects of IL-7 on tumor production of TGF-beta 1. The control cell line (FSA-Jneo) originated from the same parental fibrosarcoma cell line (FSA) and was produced by transduction with the same retroviral vector without the IL-7 gene. FSA-Jneo and FSA-JmIL-7 tumor cells were evaluated for the expression of TGF-beta 1 messenger RNA (mRNA). To determine if the observed change in TGF-beta 1 mRNA was associated with an alteration in protein secretion, we compared supernatants from tumor cell cultures for TGF-beta 1 production. Specific anti-TGF-beta 1 monoclonal antibody (MAb) was used to confirm the role of TGF-beta 1 in these assays. Results: Compared with FSA parental and FSA-Jneo cells, FSA-JmIL-7 cells expressed TGF-beta 1 mRNA at a lower level. Compared with supernatants from FSA-Jneo cells, FSA-JmIL-7 supernatants contained consistently lower levels of TGF-beta 1 activity (P<.05). In addition, FSA-Jneo supernatants suppressed lymphocyte proliferation to a significantly greater degree than supernatants from FSA-JmIL-7 cells (P<.05). Studies with anti-TGF-beta 1 MAb added to the supernatants confirmed the role of TGF-beta 1 in inhibition of lymphocyte proliferation. Conclusion: These findings suggest that IL-7 gene transfer inhibits the production of TGF-beta 1 by tumor cells and thus may enhance the efficacy of the host's antitumor immune response. Implication: The regulation of endogenous tumor-derived cytokines in response to cytokine gene transfer may contribute to altered immune responses in the tumor microenvironment and thus may be an important additional parameter to assess in gene therapy. C1 UNIV CALIF LOS ANGELES,JONSSON CANC CTR,DEPT SURG ONCOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,JONSSON CANC CTR,DEPT RADIAT ONCOL,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,MED RES SERV,LOS ANGELES,CA. BRITISH COLUMBIA CANC RES CTR,TERRY FOX LAB,VANCOUVER,BC V5Z 1L3,CANADA. RP DUBINETT, SM (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS,WADSWORTH PULM IMMUNOL LAB,LOS ANGELES,CA 90073, USA. FU NCI NIH HHS [CA5932601, CA09120, CA5899301] NR 38 TC 33 Z9 35 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD APR 19 PY 1995 VL 87 IS 8 BP 593 EP 597 DI 10.1093/jnci/87.8.593 PG 5 WC Oncology SC Oncology GA QT131 UT WOS:A1995QT13100012 PM 7752257 ER PT J AU BOMALASKI, JS FORD, T HUDSON, AP CLARK, MA AF BOMALASKI, JS FORD, T HUDSON, AP CLARK, MA TI PHOSPHOLIPASE A(2)-ACTIVATING PROTEIN INDUCES THE SYNTHESIS OF IL-1 AND TNF IN HUMAN MONOCYTES SO JOURNAL OF IMMUNOLOGY LA English DT Article ID TUMOR-NECROSIS-FACTOR; PHOSPHOLIPASE-A2-ACTIVATING PROTEIN; RHEUMATOID-ARTHRITIS; PROSTAGLANDIN BIOSYNTHESIS; ACTIVATING PROTEIN; FATTY-ACIDS; INTERLEUKIN-1; CELLS; STIMULATION; CYTOKINES AB Phospholipase A(2)-activating protein (PLAP) is an important mediator of eicosanoid generation. PLAP can also be found in high concentrations in synovial fluid from patients with rheumatoid arthritis, and injection of PLAP into animal joints results in an inflammatory, rheumatoid-like lesion. We have demonstrated previously that TNF-alpha and IL-1 beta stimulate formation of PLAP before phospholipase A(2) (PLA(2)) enzyme activation and production of eicosanoids. To further explore the mechanisms found in the inflammatory response, we examined the ability of PLAP to stimulate release of TNF and IL-1 from human peripheral blood monocytes. TNF and IL-1 protein levels were measured by ELISA, and IL-1 and TNF mRNA were determined by Northern blotting. PLAP, PLAP peptide, and melittin, a bee venom PLA(2) activator with homology with PLAP, all increased IL-1 and TNF production in a time- and dose-dependent manner. Heat-denatured PLAP and actin (an irrelevant protein) failed to exert this effect. PLAP stimulation of TNF and IL-1 could be enhanced with co-treatment of cells with free fatty acids, such as arachidonic or linoleic acid, but it was not blocked completely by PLA(2) inhibitors. These results demonstrate not only that synthesis of PLAP can be stimulated by cytokines, but also that PLAP may regulate cytokine synthesis and thus perpetuate an immune or inflammatory response. C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. SCHERING PLOUGH CORP,RES INST,KENILWORTH,NJ 07033. RP BOMALASKI, JS (reprint author), MED COLL PENN,DEPT MED,DIV RHEUMATOL,129 ANN PRESTON HALL,PHILADELPHIA,PA 19129, USA. FU NIAMS NIH HHS [AR 39382] NR 34 TC 33 Z9 33 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 1995 VL 154 IS 8 BP 4027 EP 4031 PG 5 WC Immunology SC Immunology GA QR070 UT WOS:A1995QR07000044 PM 7706741 ER PT J AU KELLER, ET ERSHLER, WB AF KELLER, ET ERSHLER, WB TI EFFECT OF IL-6 RECEPTOR ANTISENSE OLIGODEOXYNUCLEOTIDE ON IN-VITRO PROLIFERATION OF MYELOMA CELLS SO JOURNAL OF IMMUNOLOGY LA English DT Article ID HUMAN-BREAST-CARCINOMA; MULTIPLE-MYELOMA; GENE-EXPRESSION; INTERLEUKIN-6 PRODUCTION; GROWTH-INHIBITION; AUTOCRINE GROWTH; BONE-MARROW; RNASE-H; LINES; INVITRO AB IL-6 stimulates proliferation of various tumors, including lymphoma and myeloma; thus, inhibiting IL-6 may decrease the growth of these tumors. Accordingly, we examined the effect of IL-6 and IL-6R antisense phospho-rothioated oligodeoxynucleotides (ODNs) on proliferation of IL-6 responsive (U266) and nonresponsive (RPMI 8226) myeloma cell lines. Cells were grown in the presence or absence of IL-6, with added antisense to either IL-6 or IL-6R. Cells were evaluated for proliferation ([H-3]thymidine uptake) and steady state levels of both IL-6 and IL-6R mRNA by competitive PCR (C-PCR). Proliferation of U266 cells was decreased markedly by IL-6 antisense ODN in the absence of IL-6, but not in its presence. In contrast, IL-6R antisense ODN inhibited proliferation of U266 cells in both the presence and absence of IL-6. As anticipated, neither IL-6 nor IL-6R antisense ODN had an effect on RPMI 8226 proliferation. C-PCR demonstrated a marked and specific decrease of IL-6 and IL-6R mRNA in cells exposed to IL-6 ODN and IL-6R ODN, respectively. These results suggest that IL-6R antisense ODN may be a more effective inhibitor of IL-6-stimulated cells than IL-6 antisense in a therapeutic setting. C1 UNIV WISCONSIN,INST AGING,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,MADISON,WI 53705. RI Keller, Evan/M-1446-2016 OI Keller, Evan/0000-0002-7592-7535 FU NIA NIH HHS [T32 AG00213] NR 70 TC 37 Z9 37 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD APR 15 PY 1995 VL 154 IS 8 BP 4091 EP 4098 PG 8 WC Immunology SC Immunology GA QR070 UT WOS:A1995QR07000052 PM 7706747 ER PT J AU KANOFSKY, JR SIMA, PD AF KANOFSKY, JR SIMA, PD TI SINGLET OXYGEN GENERATION FROM THE REACTION OF OZONE WITH PLANT-LEAVES SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ASCORBIC-ACID; BIOMOLECULES; DEPOSITION; CELLS AB Aqueous extracts of the intercellular fluid from Sedum album L. leaves generated singlet oxygen chemiluminescence at 1270 nm when exposed to a nitrogen gas stream containing ozone at 21 +/- 2 ppm. The concentration of ascorbic acid in the intercellular fluid extracts was 310 +/- 40 mu M. The intensity of the singlet oxygen chemiluminescence from the intercellular fluid extracts was comparable with the chemiluminescence from a control solution containing 300 mu M ascorbic acid. The intensity of the singlet oxygen emission from intercellular fluid treated with ascorbate oxidase was 0.19 +/- 0.07 of the intensity of the singlet oxygen chemiluminescence from untreated samples of intercellular fluid extract. The simplest explanation for the effect of ascorbate oxidase is that ascorbic acid is the major ozone target generating singlet oxygen. Much weaker singlet oxygen chemiluminescence was detected at 1270 nm when intact S. album L. plant tips were exposed to a nitrogen gas stream containing ozone at 22 +/- 5 ppm. Various explanations for the relatively low intensity of the singlet oxygen chemiluminescence from intact S. album L. plant tips are discussed. C1 US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,RES SERV,HINES,IL 60141. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,DEPT MOLEC & CELLULAR BIOCHEM,MAYWOOD,IL 60153. RP KANOFSKY, JR (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,MED SERV,BOX 278,HINES,IL 60141, USA. NR 23 TC 55 Z9 55 U1 1 U2 7 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD APR 7 PY 1995 VL 270 IS 14 BP 7850 EP 7852 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QR526 UT WOS:A1995QR52600012 PM 7713876 ER PT J AU MEREDITH, M RABAGLIA, M METZ, S AF MEREDITH, M RABAGLIA, M METZ, S TI CYTOSOLIC BIOSYNTHESIS OF GTP AND ATP IN NORMAL RAT PANCREATIC-ISLETS SO BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH LA English DT Article DE PURINE NUCLEOTIDE INSULIN SECRETION; GTP BIOSYNTHESIS; ATP BIOSYNTHESIS; (RAT PANCREATIC ISLET) ID HUMAN NORMAL LYMPHOCYTES; MYCOPHENOLIC-ACID; IMP DEHYDROGENASE; INSULIN RELEASE; INOSINATE DEHYDROGENASE; CELLS; GUANINE; INTACT; INHIBITION; COMPARTMENTATION AB GTP and ATP are necessary for glucose-induced insulin secretion; however, the biosynthetic pathways of purine nucleotides have not been studied in pancreatic islets. The present work examines the cytosolic pathways of purine nucleotide synthesis using intact rat islets cultured overnight in RPMI 1640 medium containing either [C-14]glycine (to label the de novo pathway) or [H-3]hypoxanthine (to mark the salvage pathway), with or without mycophenolic acid or L-alanosine (selective inhibitors of cytosolic GTP and ATP synthesis, respectively). Addition of mycophenolic acid decreased total GTP content (mass) by 73-81%; although the incorporation of labeled hypoxanthine into GTP also fell by 87%, the incorporation of glycine did not change. Similarly, L-alanosine decreased ATP mass by 26-33% in the presence of either label; whereas the incorporation of hypoxanthine into ATP fell 59%, the incorporation of glycine was again not significantly decreased. Thus, both the de novo and salvage purine nucleotide biosynthetic pathways are present in rat islets; however, the salvage pathway appears to be quantitatively the more important source of nucleotides. This conclusion was supported by additional studies of the effects on nucleotide content and insulin secretion of various site-specific inhibitors of purine synthesis. These findings have potential relevance to the processes of mitogenesis, cell proliferation and differentiation of islet cells, as well as for the control of insulin secretion. C1 UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI 53792. UNIV WISCONSIN,SCH MED,ENDOCRINOL SECT,MADISON,WI 53792. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. FU NIDDK NIH HHS [DK 37312] NR 36 TC 11 Z9 11 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4889 J9 BBA-MOL CELL RES JI Biochim. Biophys. Acta-Mol. Cell Res. PD APR 6 PY 1995 VL 1266 IS 1 BP 16 EP 22 DI 10.1016/0167-4889(94)00235-7 PG 7 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA QT136 UT WOS:A1995QT13600003 PM 7718617 ER PT J AU DAVIS, PJ GREGERMAN, RI AF DAVIS, PJ GREGERMAN, RI TI PARSE ANALYSIS .2. A REVISED MODEL THAT ACCOUNTS FOR PHI SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Note C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP DAVIS, PJ (reprint author), ALBANY MED COLL,ALBANY,NY 12208, USA. NR 1 TC 4 Z9 4 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 6 PY 1995 VL 332 IS 14 BP 965 EP 966 DI 10.1056/NEJM199504063321421 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA QP893 UT WOS:A1995QP89300034 PM 7877668 ER PT J AU WILLIAMS, JW HOLLEMAN, DR SAMSA, GP SIMEL, DL AF WILLIAMS, JW HOLLEMAN, DR SAMSA, GP SIMEL, DL TI RANDOMIZED CONTROLLED TRIAL OF 3 VS 10 DAYS OF TRIMETHOPRIM-SULFAMETHOXAZOLE FOR ACUTE MAXILLARY SINUSITIS SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID ANTIMICROBIAL RESISTANCE; AMOXICILLIN-CLAVULANATE; HAEMOPHILUS-INFLUENZAE; COMPARATIVE EFFICACY; CEFUROXIME AXETIL; THERAPY; CLARITHROMYCIN; EQUIVALENCE; AMOXYCILLIN; BOOTSTRAP AB Objective.-To compare 14-day outcomes and relapse and recurrence rates among patients with acute maxillary sinusitis randomized to 3-day (3D) vs 10-day (10D) treatment with trimethoprim/sulfamethoxazole (TMP/SMX). Setting.-University-affiliated Veterans Affairs general medical and acute care clinics. Patients.-Consecutive patients with sinus symptoms and radiographic evidence of maxillary sinusitis (complete opacity, air-fluid level, or greater than or equal to 6 mm of mucosal thickening). Patients were excluded for antibiotic use within the past week, TMP/ SMX allergy, symptoms for more than 30 days, or previous sinus surgery. Methods.-All subjects (n=80) received oxymetazoline nasal spray 0.05%, two sprays twice daily for 3 days. Subjects were randomly assigned to TMP/SMX double strength: one tablet twice daily for 10 days or one tablet twice daily for 3 days followed by 7 days of placebo, At 7 and 14 days, patients rated their overall sinus symptoms on a Likert scale. Radiographs were scored at baseline and 14 days by radiologists masked to clinical symptoms and treatment assignment. The primary outcome was number of days to ''cure'' or ''much improvement'' in sinus symptoms. Patients who were clinical successes by day 14 were assessed for symptomatic relapse or recurrence at 30 and 60 days, respectively, Results.-Groups were comparable at randomization: male, 100%; black, 53%; median age, 48 years (interquartile range, 41 to 63 years); symptom duration, 10 days (interquartile range, 6 to 17 days); bilateral maxillary disease, 51%; and radiograph score, 4 (interquartile range, 2 to 4). Outcome assessment was completed in 95% of patients at day 14(n=76). Medication side effects and use of nonstudy sinus medications were equal between groups. By 14 days, 77% of 3D subjects and 76% of 10D subjects rated their sinus symptoms as cured or much improved (95% confidence interval for difference, -15% to 17%). Median days to cure/much improvement were 5.0 and 4.5 for the 3D and 10D groups, respectively; distributions of time to cure were not different (P=.34). Radiograph scores improved in both groups compared with baseline (2 points; P<.001), but improvement did not differ between groups (P=.31). Eight percent of 3D subjects and 13% of 10D subjects missed work due to sinus symptoms. Of the 52 patients who were clinical successes at 14 days and completed follow-up, three (11%) of 27 3D subjects and one (4%) of 25 10D subjects relapsed symptomatically by day 30; one (4%) of 27 3D subjects and one (4%) of 25 10D subjects suffered symptomatic recurrence between days 30 and 60 (P=.45 for the relapse and recurrence rates combined). Conclusion.-At the 2-week follow-up, clinical symptoms and radiograph scores improved equally following 3 or 10 days of TMP/SMX plus oxymetazoline nasal spray. Symptomatic relapse and recurrence were similar between groups. Three days of antibiotics were as effective as 10 days and, because of the high disease prevalence, hold the potential for substantial cost savings. C1 AUDIE L MURPHY MEM VET ADM MED CTR,DIV GEN INTERNAL MED,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. LEXINGTON VET HOSP,MED SERV,LEXINGTON,KY. LEXINGTON VET HOSP,DIV GEN INTERNAL MED,LEXINGTON,KY. UNIV KENTUCKY,LEXINGTON,KY. VET AFFAIRS MED CTR,HLTH SERV RES FIELD PROGRAM,DURHAM,NC. VET AFFAIRS MED CTR,DIV GEN INTERNAL MED,DURHAM,NC. DUKE UNIV,MED CTR,DURHAM,NC. RP WILLIAMS, JW (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,AMBULATORY CARE SERV 11C,7400 MERTON MINTON BLVD,SAN ANTONIO,TX 78284, USA. RI Williams, Jr., John/A-3696-2008 OI Williams, Jr., John/0000-0002-5267-5558 NR 48 TC 93 Z9 93 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD APR 5 PY 1995 VL 273 IS 13 BP 1015 EP 1021 DI 10.1001/jama.273.13.1015 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA QP890 UT WOS:A1995QP89000031 PM 7897784 ER PT J AU KOSOWER, E AF KOSOWER, E TI PBL AND THE LESSONS OF THE PAST SO ACADEMIC MEDICINE LA English DT Letter RP KOSOWER, E (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,SCH MED,LOS ANGELES,CA 90024, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD APR PY 1995 VL 70 IS 4 BP 253 EP 254 DI 10.1097/00001888-199504000-00002 PG 2 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA QT462 UT WOS:A1995QT46200002 PM 7718052 ER PT J AU BALLARD, RD CLOVER, CW SUH, BY AF BALLARD, RD CLOVER, CW SUH, BY TI INFLUENCE OF SLEEP ON RESPIRATORY-FUNCTION IN EMPHYSEMA SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article ID OBSTRUCTIVE PULMONARY-DISEASE; NON-REM SLEEP; VENTILATORY RESPONSE; OXYGEN DESATURATION; LUNG-VOLUME; OCCLUSION PRESSURE; ASTHMATIC-PATIENTS; CHRONIC-BRONCHITIS; MUSCLE-ACTIVITY; NORMAL HUMANS AB To assess the influence of sleep on respiratory function, five patients with chronic obstructive pulmonary disease (COPD) with laboratory evidence of emphysema were monitored during sleep in a horizontal body plethysmograph. Neither lung volume nor lower airway resistance (Rla) changed in association with sleep. Upper airway resistance (Rua) increased during sleep and was highest during rapid eye movement (REM) sleep (Rua = 5.9 +/- 1.1, 9.6 +/- 2.1, 11.2 +/- 1.5, and 15.6 +/- 4.1 cm H2O/L/s during wakefulness, Stages 2, 3-4, and REM, respectively, p < 0.05). Tidal volume (VT) decreased during sleep, resulting in a sleep-associated decrement in minute ventilation (VI = 8.69 +/- 0.46, 7.64 +/- 0.65, 7.08 +/- 0.70, and 5.62 +/- 0.47 L during wakefulness, Stages 2, 3-4, and REM, respectively, p < 0.05). Respiratory neuromuscular output was also reduced during sleep (esophageal occlusion pressure [P-0.1] = 2.69 +/- 0.39, 2.02 +/- 0.27, 1.90 +/- 0.27, and 1.63 +/- 0.25 cm H2O during wakefulness, Stages 2, 3-4, and REM, respectively, p = 0.005). We conclude that in patients with emphysema (1) sleep does not alter lung volume or increase lower airway resistance, (2) sleep is associated with a decrease in VT and VI, and (3) sleep is associated with an increase in Rua and a reduction in P-0.1 that may contribute to the sleep-associated decrease in VI. RP BALLARD, RD (reprint author), UNIV COLORADO,DENVER VET AFFAIRS MED CTR,HLTH SCI CTR,DEPT MED,DIV PULM SCI & CRIT CARE MED,DENVER,CO 80220, USA. NR 37 TC 40 Z9 45 U1 0 U2 0 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD APR PY 1995 VL 151 IS 4 BP 945 EP 951 PG 7 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA QQ888 UT WOS:A1995QQ88800005 PM 7697271 ER PT J AU HOHNLOSER, SH KLINGENHEBEN, T SINGH, BN AF HOHNLOSER, SH KLINGENHEBEN, T SINGH, BN TI AMIODARONE AND TORSADE-DE-POINTES - RESPONSE SO ANNALS OF INTERNAL MEDICINE LA English DT Letter C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP HOHNLOSER, SH (reprint author), UNIV FREIBURG,HUGSTETTER STR 55,D-79106 FREIBURG,GERMANY. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD APR 1 PY 1995 VL 122 IS 7 BP 553 EP 554 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA QP008 UT WOS:A1995QP00800015 ER PT J AU WEIZER, G MALONE, RS NETSCHER, DT WALKER, LE THORNBY, J AF WEIZER, G MALONE, RS NETSCHER, DT WALKER, LE THORNBY, J TI UTILITY OF MAGNETIC-RESONANCE-IMAGING AND ULTRASONOGRAPHY IN DIAGNOSING BREAST IMPLANT RUPTURE SO ANNALS OF PLASTIC SURGERY LA English DT Article ID COMPLICATIONS; MAMMOGRAPHY; US AB We prospectively evaluated 81 patients (with 160 implants) who subsequently had implants removed to determine sensitivity and specificity of both magnetic resonance imaging and ultrasonography. Positive and negative predictive values were also calculated to determine whether a statistically beneficial interaction existed when ultrasonography and magnetic resonance imaging were used in combination to examine an implant. Finally, the misdiagnoses were retrospectively evaluated to identify the pitfalls of the investigations. Positive diagnostic criteria were described. The sensitivity and specificity of ultrasonography were 47% and 83%, respectively, and of MRI, 46% and 88%, respectively. On retrospective review by the radiologist, the sensitivity and specificity of ultrasonography were 70% and 90%, respectively, and of magnetic resonance imaging, 75.6% and 94%, respectively. Although definite conclusions could not be obtained, there did not seem to be an additive benefit from using both ultrasonography and magnetic resonance imaging. C1 BAYLOR COLL MED,DIV DIAGNOST RADIOL,HOUSTON,TX 77030. BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. DEPT VET AFFAIRS MED CTR,HOUSTON,TX. NR 10 TC 17 Z9 18 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0148-7043 J9 ANN PLAS SURG JI Ann. Plast. Surg. PD APR PY 1995 VL 34 IS 4 BP 352 EP 361 DI 10.1097/00000637-199504000-00003 PG 10 WC Surgery SC Surgery GA QT734 UT WOS:A1995QT73400003 PM 7793779 ER PT J AU BEALL, AC LAFUENTE, JA JONES, JW AF BEALL, AC LAFUENTE, JA JONES, JW TI WEDGE RESECTION OF LUNG WITHOUT STAPLES ON SPECIMEN SO ANNALS OF THORACIC SURGERY LA English DT Note AB A technique for wedge resection of the lung using a linear cutter stapler without staples on the specimen is presented. C1 BAYLOR COLL MED,CORA WEBB DEPT SURG,HOUSTON,TX 77030. RP BEALL, AC (reprint author), HOUSTON VET AFFAIRS MED CTR,SURG SERV 112,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD APR PY 1995 VL 59 IS 4 BP 1026 EP 1026 DI 10.1016/0003-4975(94)00018-3 PG 1 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA QP487 UT WOS:A1995QP48700052 PM 7695384 ER PT J AU GRAYBILL, JR NAJVAR, LK HOLMBERG, JD LUTHER, MF AF GRAYBILL, JR NAJVAR, LK HOLMBERG, JD LUTHER, MF TI FLUCONAZOLE, D0870, AND FLUCYTOSINE TREATMENT OF DISSEMINATED CANDIDA-TROPICALIS INFECTIONS IN MICE SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article AB D0870 is a recently developed triazole with characteristics of a broad spectrum of activity and slow clearance by nonrenal mechanisms. Herein we have evaluated the efficacy of D0870, alone and combined with flucytosine, in a murine model of disseminated Candida tropicalis infection. Four isolates of C. tropicalis were evaluated. Two were highly susceptible in vitro to fluconazole, and two were resistant to fluconazole. All were highly susceptible to flucytosine and D0870. Animals were pretreated with 5-fluorouracil 1 day before infection because C. tropicalis has reduced virulence in immunocompetent mice. This was done to render them neutropenic for >10 days. Mice were infected intravenously and treated orally with D0870 or fluconazole, alone or combined with flucytosine. Survival and tissue burden of the spleen and kidneys were used to evaluate the efficacy of antifungal therapy. Fluconazole was less effective for treatment of resistant C. tropicalis than susceptible C. tropicalis. D0870 was more potent than fluconazole and was effective in fluconazole-resistant isolates. Flucytosine was consistently effective when used alone but did not consistently add to the benefit of D0870 or fluconazole. D0870 has potential in treatment of candidiasis caused by C. tropicalis, including fluconazole-resistant isolates. RP GRAYBILL, JR (reprint author), UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DIV INFECT DIS 111F,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 10 TC 29 Z9 29 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD APR PY 1995 VL 39 IS 4 BP 924 EP 929 PG 6 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA QQ123 UT WOS:A1995QQ12300024 PM 7785997 ER PT J AU SERAFETINIDES, EA AF SERAFETINIDES, EA TI CEREBRAL LATERALITY AND CONSCIOUSNESS SO ARCHIVES OF NEUROLOGY LA English DT Letter RP SERAFETINIDES, EA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. NR 4 TC 3 Z9 3 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9942 J9 ARCH NEUROL-CHICAGO JI Arch. Neurol. PD APR PY 1995 VL 52 IS 4 BP 337 EP 337 PG 1 WC Clinical Neurology SC Neurosciences & Neurology GA QR986 UT WOS:A1995QR98600001 PM 7710366 ER PT J AU HIRAYAMA, F LYMAN, SD CLARK, SC OGAWA, M AF HIRAYAMA, F LYMAN, SD CLARK, SC OGAWA, M TI THE FLT3 LIGAND SUPPORTS PROLIFERATION OF LYMPHOHEMATOPOIETIC PROGENITORS AND EARLY B-LYMPHOID PROGENITORS SO BLOOD LA English DT Article ID COLONY-STIMULATING FACTOR; TYROSINE KINASE RECEPTOR; STEM-CELL FACTOR; MULTIPOTENTIAL HEMATOPOIETIC PROGENITORS; C-KIT; GROWTH-FACTOR; INTERLEUKIN-3-DEPENDENT PROLIFERATION; DIFFERENTIATION ANTIGENS; SYNERGISTIC INTERACTIONS; MURINE HEMATOPOIESIS AB We have examined the effects of the murine ligand (FL) for the flt3/flk2 tyrosine kinase receptor on the proliferation of murine lymphohematopoietic progenitors as well as committed myeloid and B-cell progenitors. In the presence of erythropoietin, FL alone supported scant colony formation from enriched marrow cells of normal mice. However, when it was combined with interleukin-3 (IL-3), steel factor (SF), or IL-11, FL significantly enhanced colony formation. When tested on enriched marrow cells from 5-fluorouracil (5-FU)-treated mice, FL neither enhanced IL-3-dependent colony formation nor synergized with SF in support of colony formation. However, FL synergized with IL-6, IL-11, or granulocyte-colony stimulating factor (G-CSF) in support of formation of various types of colonies, including multilineage colonies. Approximately 30% of these colonies yielded pre-B-cell colonies when replated in secondary cultures containing SF and IL-7, indicating that 2-cytokine combinations, including FL and IL-6, IL-11, or G-CSF can support the proliferation of primitive lymphohematopoietic progenitors. FL, by itself and in synergy with IL-7 or SF, supported the proliferation of B-cell progenitors. These results show that FL has a wide range of activities in early hematopoiesis and B lymphopoiesis. (C) 1995 by The American Society of Hematology. C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. GENET INST INC,CAMBRIDGE,MA. IMMUNEX CORP,SEATTLE,WA. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC 29401. FU NIDDK NIH HHS [DK 32294] NR 53 TC 147 Z9 147 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0006-4971 J9 BLOOD JI Blood PD APR 1 PY 1995 VL 85 IS 7 BP 1762 EP 1768 PG 7 WC Hematology SC Hematology GA QP613 UT WOS:A1995QP61300010 PM 7703483 ER PT J AU LEVINE, SM BRYAN, CL AF LEVINE, SM BRYAN, CL TI BRONCHIOLITIS OBLITERANS IN LUNG-TRANSPLANT RECIPIENTS - THE THORN IN THE SIDE OF LUNG TRANSPLANTATION SO CHEST LA English DT Editorial Material ID ALLOGRAFTS; REJECTION C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV PULM DIS CRIT CARE MED,SAN ANTONIO,TX. RP LEVINE, SM (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,PULM DIS SECT 111 E,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 15 TC 17 Z9 17 U1 0 U2 0 PU AMER COLL CHEST PHYSICIANS PI NORTHBROOK PA 3300 DUNDEE ROAD, NORTHBROOK, IL 60062-2348 SN 0012-3692 J9 CHEST JI Chest PD APR PY 1995 VL 107 IS 4 BP 894 EP 897 DI 10.1378/chest.107.4.894 PG 4 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA QR617 UT WOS:A1995QR61700005 PM 7705149 ER PT J AU RHO, JP YOSHIKAWA, TT AF RHO, JP YOSHIKAWA, TT TI THE COST OF INAPPROPRIATE USE OF ANTIINFECTIVE AGENTS IN OLDER PATIENTS SO DRUGS & AGING LA English DT Article ID ADVERSE DRUG-REACTIONS; SKILLED-NURSING FACILITIES; ANTIBIOTIC USE; ANTIMICROBIAL RESISTANCE; GERIATRIC-PATIENTS; HOME RESIDENTS; MEDICATION; USAGE; COMMUNITY; AMINOGLYCOSIDES C1 US DEPT VET AFFAIRS,OFF GERIATR & EXTENDED CARE,WASHINGTON,DC 20420. GEORGE WASHINGTON UNIV,SCH MED,DEPT HLTHCARE SCI,WASHINGTON,DC. UNIV SO CALIF,SCH PHARM,DEPT CLIN PHARM,LOS ANGELES,CA. NR 43 TC 5 Z9 5 U1 0 U2 0 PU ADIS INTERNATIONAL LTD PI AUCKLAND PA 41 CENTORIAN DR, PRIVATE BAG 65901, MAIRANGI BAY, AUCKLAND 10, NEW ZEALAND SN 1170-229X J9 DRUG AGING JI Drugs Aging PD APR PY 1995 VL 6 IS 4 BP 263 EP 267 DI 10.2165/00002512-199506040-00001 PG 5 WC Geriatrics & Gerontology; Pharmacology & Pharmacy SC Geriatrics & Gerontology; Pharmacology & Pharmacy GA QR191 UT WOS:A1995QR19100001 PM 7613015 ER PT J AU TAKAHASHI, S REDDY, SV DALLAS, M DEVLIN, R CHOU, JY ROODMAN, GD AF TAKAHASHI, S REDDY, SV DALLAS, M DEVLIN, R CHOU, JY ROODMAN, GD TI DEVELOPMENT AND CHARACTERIZATION OF A HUMAN MARROW STROMAL CELL-LINE THAT ENHANCES OSTEOCLAST-LIKE CELL-FORMATION SO ENDOCRINOLOGY LA English DT Article ID HUMAN-BONE-MARROW; COLONY-STIMULATING FACTOR; MULTINUCLEATED CELLS; GROWTH-FACTOR; OSTEOPETROTIC MICE; OSTEOBLASTIC CELLS; MOLECULAR-CLONING; SIMIAN VIRUS-40; GENE-EXPRESSION; SPLEEN-CELLS AB We established a human bone marrow stromal cell line (Saka) by infecting marrow adherent cells from semisolid marrow cultures with a recombinant simian virus-40 (SV40) virus. The cells expressed SV40 large tumor antigen, had a fibroblast-like shape, and expressed fibronectin and vimentin. They did not contain detectable alkaline phosphatase activity; express myeloid, lymphoid, or factor VIII-associated antigens; or develop adipocyte-like characteristics with dexamethasone treatment. Polymerase chain reaction analysis of Saka cell RNA detected expression of messenger RNAs for interleukin-6 (IL-6), IL-1 beta, granulocyte-macrophage colony-stimulating factor, macrophage colony-stimulating factor, stem cell factor, and the 1,25-dihydroxyvitamin D-3 receptor. Coculture of Saka cells with human marrow mononuclear cells enhanced formation of osteoclast-like multinucleated cells (MNC) in long term human bone marrow cultures. These MNC expressed calcitonin receptors and formed resorption lacunae on dentine. In contrast, coculture of marrow mononuclear cells with other SV40-transformed human marrow stromal cell lines did not increase MNC formation. Conditioned medium from Saka cells or coculture of bone marrow and Saka cells separated by a Millipore membrane did not enhance MNC formation. Addition of a neutralizing antibody to IL-6 or IL-1 beta blocked the effects of Saka cells on MNC formation. These results suggest that marrow stromal cells enhance osteoclast formation in part through direct cell to cell contact and production of IL-6 and/or IL-1 beta. C1 AUDIE L MURPHY MEM VET ADM MED CTR, RES SERV 151, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT MED, SAN ANTONIO, TX 78284 USA. NIH, BETHESDA, MD 20892 USA. FU NCI NIH HHS [CA-40035]; NIADDK NIH HHS [AM-35188]; NIAMS NIH HHS [AR-39539] NR 44 TC 33 Z9 34 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD APR PY 1995 VL 136 IS 4 BP 1441 EP 1449 DI 10.1210/en.136.4.1441 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QP117 UT WOS:A1995QP11700017 PM 7534699 ER PT J AU HISNANICK, JJ KYER, BL AF HISNANICK, JJ KYER, BL TI ASSESSING A DISAGGREGATED ENERGY INPUT - USING CONFIDENCE-INTERVALS AROUND TRANSLOG ELASTICITY ESTIMATES SO ENERGY ECONOMICS LA English DT Article DE ENERGY; ELASTICITY ESTIMATES; AES ID SUBSTITUTION; DEMAND; GROWTH AB The role of energy in the production of manufacturing output has been debated extensively in the literature, particularly its relationship with capital and labor. In an attempt to provide some clarification in this debate, a two-step methodology was used. First, under the assumption of a five-factor production function specification, we distinguished between electric and non-electric energy and assessed each component's relationship with capital and labor. Second, we calculated both the Allen and price elasticities and constructed 95% confidence intervals around these values. Our approach led to the following conclusions: that the disaggregation of the energy input into electric and non-electric energy is justified; that capital and electric energy and capital and non-electric energy are substitutes, while labor and electric energy and labor and non-electric energy are complements in production; and that capital and energy are substitutes, while labor and energy are complements. C1 FRANCIS MARION COLL,FLORENCE,SC 29501. RP HISNANICK, JJ (reprint author), US DEPT VET AFFAIRS,NCVAS 008C12,810 VERMONT AVE NW,WASHINGTON,DC 20420, USA. NR 18 TC 13 Z9 14 U1 1 U2 4 PU BUTTERWORTH-HEINEMANN LTD PI OXFORD PA LINACRE HOUSE JORDAN HILL, OXFORD, OXON, ENGLAND OX2 8DP SN 0140-9883 J9 ENERG ECON JI Energy Econ. PD APR PY 1995 VL 17 IS 2 BP 125 EP 132 DI 10.1016/0140-9883(95)00008-I PG 8 WC Economics SC Business & Economics GA RE974 UT WOS:A1995RE97400004 ER PT J AU CHRISTENSEN, EI NIELSEN, S MOESTRUP, SK BORRE, C MAUNSBACH, AB DEHEER, E RONCO, P HAMMOND, TG VERROUST, P AF CHRISTENSEN, EI NIELSEN, S MOESTRUP, SK BORRE, C MAUNSBACH, AB DEHEER, E RONCO, P HAMMOND, TG VERROUST, P TI SEGMENTAL DISTRIBUTION OF THE ENDOCYTOSIS RECEPTOR GP330 IN RENAL PROXIMAL TUBULES SO EUROPEAN JOURNAL OF CELL BIOLOGY LA English DT Article DE KIDNEY; IMMUNOCYTOCHEMISTRY; LYSOSOMES; ENDOCYTOSIS; RECEPTORS ID DENSITY-LIPOPROTEIN RECEPTOR; FOLATE-BINDING PROTEIN; BRUSH-BORDER; HEYMANN NEPHRITIS; COATED PIT; RAT-KIDNEY; ULTRASTRUCTURAL-LOCALIZATION; MONOCLONAL-ANTIBODIES; EPITHELIAL-CELLS; PLASMA-MEMBRANE AB The subcellular distribution and segmental variations in location of gp330, a scavenger receptor for filtered proteins in renal proximal tubules, was analyzed. Kidney tissue from rats (4 differed strains), rabbits and humans were analyzed by light- and electron microscope immunocytochemistry, using cryosections or Lowicryl sections from cryosubstituted tissue. Gp330 was located mainly in apical coated pits, small and large endocytic vacuoles and in dense apical tubules in the proximal tubule cells. The labeling density was markedly higher in segments 1 and 2 as compared to segment 3 of the proximal tubule. In addition to the location in the early part of the endocytic pathway, gp330 was also present in lysosomes, especially in segments 1 and 2. The lysosomal labeling was not restricted to the membrane, but was also seen in the matrix. Localization of gp330 in lysosomes was ron firmed on sections from purified lysosomal fractions from rat renal cor tex. The brash border localization of gp330 in proximal tubules exhibited a characteristic segmental variation. In the initial part of segment 1, there was virtually no brush border labeling, In the remaining part of segment 1 and in segment 2, there was a distinct but sometimes patchy labeling of the brush border. In segment 3, groups of microvilli of approximately 10 as seen in sections were intensively labeled from bottom to tip and there were often more than one of these groups on a single cell, the remaining microvilli were unlabeled. No differences in the cellular and subcellular localization of gp330 were observed between species or rat strains. In conclusion, the present study demonstrates that in addition to its location in the early endocytic and recycling pathway, gp330 is also present in microvilli and the protein and degradation products thereof is present in lysosomes, consistent with its role as a protein scavenger receptor. C1 AARHUS UNIV,INST MED BIOCHEM,DK-8000 AARHUS,DENMARK. LEIDEN UNIV,DEPT PATHOL,LEIDEN,NETHERLANDS. HOP TENON,INSERM,U64,F-75970 PARIS,FRANCE. UNIV WISCONSIN HOSP & CLIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,MADISON,WI 53792. RP CHRISTENSEN, EI (reprint author), AARHUS UNIV,INST ANAT,DEPT CELL BIOL,DK-8000 AARHUS C,DENMARK. RI Moestrup, Soren Kragh/A-1403-2014 OI Moestrup, Soren Kragh/0000-0003-3862-2107 FU NIDDK NIH HHS [DK 46117] NR 49 TC 118 Z9 118 U1 0 U2 2 PU WISSENSCHAFTLICHE VERLAG GMBH PI STUTTGART 10 PA BIRKENWALDSTRASSE 44 POSTFACH 105339, W-7000 STUTTGART 10, GERMANY SN 0171-9335 J9 EUR J CELL BIOL JI Eur. J. Cell Biol. PD APR PY 1995 VL 66 IS 4 BP 349 EP 364 PG 16 WC Cell Biology SC Cell Biology GA QT314 UT WOS:A1995QT31400005 PM 7656901 ER PT J AU CARMEL, R GOTT, PS WATERS, CH CAIRO, K GREEN, R BONDAREFF, W DEGIORGIO, CM CUMMINGS, JL JACOBSEN, DW BUCKWALTER, G HENDERSON, VW AF CARMEL, R GOTT, PS WATERS, CH CAIRO, K GREEN, R BONDAREFF, W DEGIORGIO, CM CUMMINGS, JL JACOBSEN, DW BUCKWALTER, G HENDERSON, VW TI THE FREQUENTLY LOW COBALAMIN LEVELS IN DEMENTIA USUALLY SIGNIFY TREATABLE METABOLIC, NEUROLOGIC AND ELECTROPHYSIOLOGIC ABNORMALITIES SO EUROPEAN JOURNAL OF HAEMATOLOGY LA English DT Article DE COBALAMIN DEFICIENCY; METHYLMALONIC ACID; HOMOCYSTEINE; EVOKED POTENTIALS; ELECTROENCEPHALOGRAM; DEMENTIA MALABSORPTION ID DEOXYURIDINE SUPPRESSION TEST; LOW SERUM VITAMIN-B12; ALZHEIMERS-DISEASE; MEGALOBLASTIC-ANEMIA; DEFICIENCY STATES; PERNICIOUS-ANEMIA; SENILE DEMENTIA; FOLLOW-UP; SUBTLE; MANIFESTATIONS AB Cobalamin levels are frequently low in patients with dementia, but it is unclear if they represent definable deficiency and what the mechanisms are. Therefore, patients being evaluated for dementia who had low cobalamin levels but no obvious evidence of deficiency were studied hematologically, neurologically and with metabolic tests and were re-evaluated after cobalamin treatment. Abnormalities suggestive of or diagnostic for deficiency were documented in most of the 16 demented and nondemented patients. Metabolic results: 50% of patients tested had abnormal deoxyuridine suppression and 44% had increased serum methylmalonic acid and/or homocysteine levels; these test results correlated with each other. Neurologic results: 73% of patients had clinical abnormalities, primarily mild neuropathies, not attributable to other causes, 75% had electroencephalographic abnormalities, 77% had abnormal visual evoked potentials and 33% had abnormal somatosensory potentials. Metabolic and neurologic dysfunction were present together or absent together in all but 2 cases. Cobalamin therapy improved 50-100% of the various types of abnormalities, although it did not improve cognitive function in the 13 demented patients. Food-cobalamin malabsorption was found in 60% of the patients. Despite the absence of megaloblastic anemia and rarity of traditional malabsorption of free cobalamin, low cobalamin levels in demented patients frequently represent mild cobalamin deficiency and are often associated with food-cobalamin malabsorption. Perhaps most importantly, this is accompanied not only by metabolic changes but by evidence of mild neurologic dysfunction. Their frequent reversibility by cobalamin confirms that these defects indeed arise from cobalamin deficiency. Although the long-standing dementia does not improve, treating such patients with cobalamin has other concrete benefits. C1 UNIV SO CALIF,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90033. UNIV SO CALIF,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90033. UNIV SO CALIF,LOS ANGELES CTY MED CTR,LOS ANGELES,CA 90033. CLEVELAND CLIN FDN,LAB HEMATOL SECT,CLEVELAND,OH 44195. UNIV CALIF LOS ANGELES,HLTH SCI CTR,DEPT NEUROL,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,HLTH SCI CTR,DEPT PSYCHIAT,LOS ANGELES,CA. UNIV SO CALIF,DEPT PSYCHOL,LOS ANGELES,CA 90089. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,LOS ANGELES,CA 90073. UNIV SO CALIF,ANDRUS GERONTOL CTR,LOS ANGELES,CA. RP CARMEL, R (reprint author), UNIV SO CALIF,SCH MED,DEPT MED,RMR 306,2025 ZONAL AVE,LOS ANGELES,CA 90033, USA. FU NCRR NIH HHS [MO1 RR-43]; NIA NIH HHS [AG-05142]; NIDDK NIH HHS [DK-32640] NR 51 TC 97 Z9 97 U1 0 U2 2 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0902-4441 J9 EUR J HAEMATOL JI Eur. J. Haematol. PD APR PY 1995 VL 54 IS 4 BP 245 EP 253 PG 9 WC Hematology SC Hematology GA RD188 UT WOS:A1995RD18800006 PM 7789470 ER PT J AU AMIR, I SHARMA, R BAUMAN, WA KORSTEN, MA AF AMIR, I SHARMA, R BAUMAN, WA KORSTEN, MA TI BOWEL CARE IN SPINAL-CORD INJURY PATIENTS - A COMPARISON OF 4 MODALITIES SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 BRONX VET AFFAIRS MED CTR,SPINAL CORD DAMAGE RES CTR,GASTROENTEROL SECT,BRONX,NY. CUNY MT SINAI SCH MED,NEW YORK,NY 10029. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A562 EP A562 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302235 ER PT J AU COHEN, J SAVIDES, TS JENSEN, DM AF COHEN, J SAVIDES, TS JENSEN, DM TI SURVIVAL OF PATIENTS WITH SEVERE UGI BLEEDING FROM GASTRIC ADENOCARCINOMA SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VAMC,CURE,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A457 EP A457 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86301816 ER PT J AU DEA, SK YOUNG, SH ENNES, HS MAYER, EA AF DEA, SK YOUNG, SH ENNES, HS MAYER, EA TI SUBSTANCE-P PRODUCES OSCILLATIONS OF INTRACELLULAR CALCIUM ([CA](I)) IN MYOCYTES FROM LONGITUDINAL (L) BUT NOT CIRCULAR (C) COLONIC MUSCLE SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, DEPT PHYSIOL, CURE VA UCLA GASTROENTER BIOL CTR NEUROENTER BIOL, LOS ANGELES, CA USA. UNIV CALIF LOS ANGELES, DEPT MED, LOS ANGELES, CA 90073 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A589 EP A589 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302344 ER PT J AU DIEHL, DL FULLERTON, S MAYER, EA AF DIEHL, DL FULLERTON, S MAYER, EA TI FUNCTIONAL BOWEL-DISEASE SYMPTOMS IN PATIENTS WITH CHRONIC FATIGUE SYNDROME (CFS) - PREVALENCE AND QUALITY-OF-LIFE SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT MED, CURE VA UCLA GASTROENTER BIOL CTR, LOS ANGELES, CA USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A592 EP A592 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302356 ER PT J AU FASS, R FULLERTON, S DIEHL, DL HIRSH, T MAYER, EA AF FASS, R FULLERTON, S DIEHL, DL HIRSH, T MAYER, EA TI SEXUAL DYSFUNCTION IN PATIENTS WITH IRRITABLE-BOWEL-SYNDROME (IBS) AND NONULCER DYSPEPSIA (NUD) SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT MED, CURE VA UCLA GASTROENTER BIOL CTR NEUROENTER BIOL, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A596 EP A596 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302371 ER PT J AU FASS, R FULLERTON, S DIEHL, DL MAYER, EA AF FASS, R FULLERTON, S DIEHL, DL MAYER, EA TI SLEEP DISTURBANCE (SDE IN PATIENTS WITH FUNCTIONAL BOWEL DISORDERS (FBD) SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, DEPT MED CURE VA UCLA GASTROENTER BIOL CTR NEUROEN, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A596 EP A596 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302373 ER PT J AU FASS, R KAGAN, B FULLERTON, S MAYER, EA AF FASS, R KAGAN, B FULLERTON, S MAYER, EA TI THE PREVALENCE OF FUNCTIONAL GASTROINTESTINAL SYMPTOMS IN MALE-PATIENTS WITH POSTTRAUMATIC-STRESS-DISORDER (PTSD) SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA 90073 USA. CURE VA UCLA GASTROENTER BIOL CTR, NEUROENTER BIOL GRP, LOS ANGELES, CA 90073 USA. NR 1 TC 9 Z9 9 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A597 EP A597 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302374 ER PT J AU FULLERTON, S CONTEAS, C DONOVAN, J NALIBOFF, B MAYER, EA AF FULLERTON, S CONTEAS, C DONOVAN, J NALIBOFF, B MAYER, EA TI MEASURING SEVERITY OF ILLNESS FOR PATIENTS WITH FUNCTIONAL BOWEL-DISEASE USING SENSORY AND AFFECTIVE VERBAL DESCRIPTOR RATING-SCALES SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 SO CALIF PERMANENTE MED GRP, LOS ANGELES, CA USA. W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT MED, CURE VA UCLA, GASTROENTER BIOL CTR NEUROENTER BIOL GRP, LOS ANGELES, CA 90073 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A602 EP A602 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302397 ER PT J AU FULLERTON, S MERTZ, H DIEHL, DL MAYER, EA AF FULLERTON, S MERTZ, H DIEHL, DL MAYER, EA TI A COMPARISON OF DIAGNOSTIC-CRITERIA FOR IRRITABLE-BOWEL-SYNDROME SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, DEPT MED, CURE VA UCLA GASTROENTER BIOL CTR NEUROENTER BIOL, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A602 EP A602 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302395 ER PT J AU JACOBY, RF VERMA, AK TUTSCH, KD MAMBY, CA LOVE, RR AF JACOBY, RF VERMA, AK TUTSCH, KD MAMBY, CA LOVE, RR TI PLACEBO-CONTROLLED RANDOMIZED TRIAL OF DFMO AS A CHEMOPREVENTIVE AGENT IN PATIENTS AT HIGH-RISK FOR COLORECTAL-CANCER SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,CTR COMPREHENS CANC,DEPT HUMAN ONCOL,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A485 EP A485 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86301930 ER PT J AU JACOBY, RF MARSHALL, DJ NOVAKOVIC, KR FRICK, T MOSER, AR DOVE, WF AF JACOBY, RF MARSHALL, DJ NOVAKOVIC, KR FRICK, T MOSER, AR DOVE, WF TI COLON-CANCER CHEMOPREVENTION STUDIES USING THE APC MUTANT MIN/+ ONCOMOUSE SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. UNIV WISCONSIN,DEPT ONCOL,MADISON,WI 53706. UNIV WISCONSIN,DEPT GENET,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. NR 0 TC 0 Z9 0 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A485 EP A485 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86301928 ER PT J AU LLOYD, M HURLEY, C KORSMO, H OLSEN, W AF LLOYD, M HURLEY, C KORSMO, H OLSEN, W TI TRANS-ACTING NUCLEAR PROTEINS IN HUMAN LACTASE AND SUCRASE-ISOMALTASE REGULATION SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. NR 2 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A736 EP A736 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302935 ER PT J AU MERTZ, H FULLERTON, S HIRSH, T MAYER, EA AF MERTZ, H FULLERTON, S HIRSH, T MAYER, EA TI CLASSIFICATION OF CONSTIPATION BY BIOLOGICAL PARAMETERS AND SYMPTOM CRITERIA SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT MED,CURE,GASTROENTER BIOL CTR,NEUROENTER BIOL GRP,LOS ANGELES,CA 90024. VANDERBILT UNIV,DEPT MED,NASHVILLE,TN 37240. NR 0 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A648 EP A648 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302582 ER PT J AU MUNAKATA, J CHANG, L AN, C NALIBOFF, B MAYER, EA AF MUNAKATA, J CHANG, L AN, C NALIBOFF, B MAYER, EA TI REPETITIVE ACTIVATION OF SIGMOID MECHANORECEPTORS RESULTS IN THE DEVELOPMENT OF RECTAL HYPERALGESIA IN IBS PATIENTS SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, CURE VA UCLA GASTROENTER BIOL CTR NEUROBIOL GRP, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, HARBOR MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A653 EP A653 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302599 ER PT J AU MUNAKATA, J NALIBOFF, B CHANG, L AN, C MAYER, EA AF MUNAKATA, J NALIBOFF, B CHANG, L AN, C MAYER, EA TI OCTREOTIDE INHIBITS THE DEVELOPMENT OF RECTAL HYPERALGESIA IN RESPONSE TO MECHANICAL SIGMOID SENSITIZATION IN IBS PATIENTS SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES, HARBOR MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS, MED CTR, DEPT MED, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, W LOS ANGELES VET AFFAIRS MED CTR, CURE VA UCLA GASTROENTER BIOL, CTR NEUROENTEROL BIOL GRP, LOS ANGELES, CA 90073 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A652 EP A652 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302598 ER PT J AU NALIBOFF, B HIRSH, T KODNER, A NIAZI, N MAYER, EA AF NALIBOFF, B HIRSH, T KODNER, A NIAZI, N MAYER, EA TI PHYSIOLOGICAL AND SYMPTOMATIC IMPROVEMENT FOLLOWING AN ANORECTAL BIOFEEDBACK TRAINING-PROGRAM FOR INCONTINENCE AND CONSTIPATION - COMPARISON OF PATIENTS WITH AND WITHOUT IBS SYMPTOMS SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,CURE VA UCLA GASTROENTER BIOL CTR NEUROENTER BIOL GRP,LOS ANGELES,CA 90024. NR 0 TC 4 Z9 4 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A656 EP A656 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86302613 ER PT J AU TORBEY, C KORSMO, H OLSEN, WA AF TORBEY, C KORSMO, H OLSEN, WA TI REGULATION OF LACTASE EXPRESSION IN A HUMAN INTESTINAL-CELL LINE AND IN RATS IS INDEPENDENT OF PUTATIVE TRANSCRIPTION FACTOR NF-LPH1 SO GASTROENTEROLOGY LA English DT Meeting Abstract C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,GASTROENTEROL RES LAB,MADISON,WI 53705. UNIV WISCONSIN,DEPT MED,MADISON,WI 53706. NR 1 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD APR PY 1995 VL 108 IS 4 SU S BP A758 EP A758 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA QT863 UT WOS:A1995QT86303019 ER PT J AU WIEDERHOLD, ML YAMASHITA, M LARSEN, KA BATTEN, JS KOIKE, H ASASHIMA, M AF WIEDERHOLD, ML YAMASHITA, M LARSEN, KA BATTEN, JS KOIKE, H ASASHIMA, M TI DEVELOPMENT OF THE OTOLITH ORGANS AND SEMICIRCULAR CANALS IN THE JAPANESE RED-BELLIED NEWT, CYNOPS-PYRRHOGASTER SO HEARING RESEARCH LA English DT Article DE OTOCONIA; OTOLITH; UTRICLE; SACCULE; SEMICIRCULAR CANALS; DEVELOPMENT ID X-RAY-DIFFRACTION; OTOCONIA AB The sequence in which the otoliths and semicircular canals and their associated sensory epithelia appear and develop in the newt are described. Three-dimensional reconstruction of serial sections through the otic vesicle of newt embryos from stages 31 through 58 demonstrate the first appearance, relative position and growth of the otoliths. A single otolith is first seen in stage 33 embryos (approximately 9 days old); this splits into separate utricular and saccular otoliths at stage 40 (13 days). The lateral semicircular canal is the first to appear, at stage 41 (14 days). The anterior and posterior canals appear approximately one week later and the vestibular apparatus is essentially fully formed at stage 58 (approximately 5 weeks). The data reported here will serve as ground-based controls for fertilized newt eggs flown on the International Microgravity Laboratory-2 Space Shuttle night, to investigate the influence of microgravity on the development of the gravity-sensing organs. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. INST SPACE & ASTRONAUT SCI,SAGAMIHARA,KANAGAWA 229,JAPAN. UNIV TOKYO,TOKYO,JAPAN. RP WIEDERHOLD, ML (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 25 TC 10 Z9 10 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5955 J9 HEARING RES JI Hear. Res. PD APR PY 1995 VL 84 IS 1-2 BP 41 EP 51 DI 10.1016/0378-5955(95)00012-S PG 11 WC Audiology & Speech-Language Pathology; Neurosciences; Otorhinolaryngology SC Audiology & Speech-Language Pathology; Neurosciences & Neurology; Otorhinolaryngology GA QV812 UT WOS:A1995QV81200005 PM 7642454 ER PT J AU STEYGER, PS WIEDERHOLD, ML BATTEN, J AF STEYGER, PS WIEDERHOLD, ML BATTEN, J TI THE MORPHOGENIC FEATURES OF OTOCONIA DURING LARVAL DEVELOPMENT OF CYNOPS-PYRRHOGASTER, THE JAPANESE RED-BELLIED NEWT SO HEARING RESEARCH LA English DT Article DE OTOCONIA; VESTIBULAR; MORPHOGENESIS; BIOMINERALIZATION; EXTRACELLULAR MATRIX ID ENDOLYMPHATIC SAC; CRYSTAL-GROWTH; PROTEIN; STATOCONIA; MEMBRANE AB Otoconia are calcified protein matrices within the gravity-sensing organs of the vertebrate vestibular system. Mammalian otoconia are barrel-shaped with triplanar facets at each end. Reptilian otoconia are commonly prismatic or fusiform in shape. Amphibians have all three otoconial morphologies, barrel-shaped otoconia within the utricle, with prismatic and fusiform otoconia in the saccule. Scanning electron microscopy revealed a sequential appearance of all three otoconial morphologies during larval development of the newt, Cynops pyrrhogaster. The first otoconia appear within a single, developing otolith, and some resemble adult barrel-shaped otoconia. As the larvae hatch, around stages 39-42, the single otolith divides into two anatomically separate regions, the utricle and saccule, and both contain otoconia similar to those seen in the single otolith. Throughout development, these otoconia may have variable morphologies, with serrated surfaces, or circumferential striations with either separated facets or adjacent facets in the triplanar end-regions. small fusiform otoconia occur later, at stage 51, and only in the saccule. Prismatic otoconia appear later still, at stage 55, and again only in the saccule. Thus, although prismatic otoconia are the most numerous in adult newts, it is the last vestibular otoconial morphology to be expressed. C1 UNIV TEXAS,HLTH SCI CTR,DEPT OTOLARYNGOL HEAD & NECK SURG,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 50 TC 15 Z9 15 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5955 J9 HEARING RES JI Hear. Res. PD APR PY 1995 VL 84 IS 1-2 BP 61 EP 71 DI 10.1016/0378-5955(95)00013-T PG 11 WC Audiology & Speech-Language Pathology; Neurosciences; Otorhinolaryngology SC Audiology & Speech-Language Pathology; Neurosciences & Neurology; Otorhinolaryngology GA QV812 UT WOS:A1995QV81200007 PM 7642456 ER PT J AU GOODFRIEND, TL BALL, DL ELLIOTT, ME SHACKLETON, C AF GOODFRIEND, TL BALL, DL ELLIOTT, ME SHACKLETON, C TI LEAD INCREASES ALDOSTERONE PRODUCTION BY RAT ADRENAL-CELLS SO HYPERTENSION LA English DT Article; Proceedings Paper CT 48th Annual Fall Conference and Scientific Sessions of the Council-for-High-Blood-Pressure-Research CY SEP 27-30, 1994 CL CHICAGO, IL SP COUNCIL HIGH BLOOD PRESSURE RES DE LEAD; BLOOD PRESSURE; ALDOSTERONE; HYPERTENSION, MINERALOCORTICOID; CORTICOSTERONE; ADRENAL GLANDS ID BLOOD-PRESSURE; ANGIOTENSIN; EXPOSURE AB Exposure to lead has been postulated to contribute to elevated blood pressure in humans and has been shown to raise blood pressure in animals. The mechanism of action of lead on blood pressure is unknown. We fed lead to rats in their drinking water and then examined the production of aldosterone by their adrenal cells in vitro. We also measured excretion of aldosterone and corticosterone by intact rats stimulated with corticotropin, with and without lead treatment. At a dose (273 ppm) that raised blood levels to 30 to 40 mu g/dL, comparable to blood levels in exposed humans, lead induced increased aldosterone secretion in vitro and in vivo. The effect of lead was most evident when cells or animals were stimulated with aldosterone secretagogues. Experiments in vitro indicate that exposure to lead in vivo increases activity of one or more steps in the late pathway of aldosterone biosynthesis. The results suggest that the hypertensive effect of lead involves relative hyperaldosteronism and may be most evident when secretion of this hormone is stimulated. C1 UNIV WISCONSIN,DEPT PHARMACOL,MADISON,WI. UNIV WISCONSIN,DEPT MED,MADISON,WI. CHILDRENS HOSP RES INST,OAKLAND,CA. RP GOODFRIEND, TL (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. FU NIDDK NIH HHS [DK34400] NR 14 TC 16 Z9 16 U1 0 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0194-911X J9 HYPERTENSION JI Hypertension PD APR PY 1995 VL 25 IS 4 BP 785 EP 789 PN 2 PG 5 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA QT942 UT WOS:A1995QT94200025 PM 7721433 ER PT J AU BENNETT, CL ADAMS, J BENNETT, RL RODRIQUE, D GEORGE, L CASSILETH, B GILMAN, SC AF BENNETT, CL ADAMS, J BENNETT, RL RODRIQUE, D GEORGE, L CASSILETH, B GILMAN, SC TI THE LEARNING-CURVE FOR AIDS-RELATED PNEUMOCYSTIS-CARINII PNEUMONIA - EXPERIENCE FROM 3,981 CASES IN VETERANS AFFAIRS HOSPITALS 1987-1991 SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE PNEUMOCYSTIS CARINII PNEUMONIA; LEARNING CURVE; SELECTIVE REFERRAL ID ACQUIRED IMMUNODEFICIENCY SYNDROME; SURVIVAL; MORTALITY; PATTERNS AB Previous studies have found lower mortality rates for AIDS-related Pneumocystis carinii pneumonia (PCP) in hospitals with higher levels of experience with PCP. It is not known if patients are selectively referred to better hospitals or if there is a learning curve whereby outcomes improve as physicians gain experience in treating PCP. We assessed cases of PCP at 140 Veterans Administration (VA) Medical Centers in the United States. During 1987-1991, 3,981 patients were hospitalized with first-episode AIDS-related PCP. Mortality at 30 days after admission. For these 3,981 hospitalizations at the 140 study hospitals, the 30-day mortality was 19%. Logistic regression models indicate that older age, race, geographic area, earlier year of treatment, hospitalization in the previous 12 months, and lower levels of hospital experience with AIDS were significant predictors of mortality at 30 days after admission. Compared with hospitals that had treated three cases or fewer of first-episode PCP, the odds of mortality at 30 days at hospitals that treated >50 cases of first-episode PCP were 0.73 (95% confidence interval 0.58-0.91), after controlling for differences in characteristics of the patients, year, and region. Mortality of patients with AIDS-related PCP decreases as VA hospitals gain experience. Longitudinal analyses over a 5-year period suggest that a learning curve best explains this finding. C1 DURHAM VET ADM HOSP,DIV HLTH SERV RES,DURHAM,NC. DUKE UNIV,DIV HEMATOL ONCOL,DURHAM,NC. DUKE UNIV,CTR HLTH POLICY RES & EDUC,DURHAM,NC. DUKE UNIV,DEPT MED,DURHAM,NC. DUKE UNIV,CTR CANC,DURHAM,NC. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. RAND CORP,LONG BEACH,CA. VET ADM MED CTR,WESTERN REG SPECIAL STUDIES GRP,LONG BEACH,CA 90822. UNIV SO CALIF,DIV INFECT DIS,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. VET AFFAIRS MED CTR,LONG BEACH,CA. RI Bennett, Charles/C-2050-2008 FU AHRQ HHS [1R01HS06494-01] NR 17 TC 19 Z9 19 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PD APR 1 PY 1995 VL 8 IS 4 BP 373 EP 378 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA QL406 UT WOS:A1995QL40600008 PM 7882102 ER PT J AU HEFLE, SL HELM, RM BURKS, AW BUSH, RK AF HEFLE, SL HELM, RM BURKS, AW BUSH, RK TI COMPARISON OF COMMERCIAL PEANUT SKIN-TEST EXTRACTS SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE PEANUT ALLERGY; SKIN TESTING; ALLERGENIC EXTRACTS ID ATOPIC-DERMATITIS; ALLERGEN; PROTEINS; IDENTIFICATION AB Background: Skin prick testing is a major tool for diagnosing food allergy. Food allergen extracts have not been standardized; this may lead to great variability in the predictive accuracy of skin prick tests. Methods: Six commercial peanut skin test extracts were compared in vitro with RAST inhibition assays, ELISA, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by immunoblotting with sera from peanut-allergic adults and in vivo by skin prick testing. Results: ELISA showed that the content of peanut allergens Ara h I and Ara h II in the extracts ranged from 0.0015 to 0.0236 and 0.0001 to 0.0164 mg Eq/ml, respectively. RAST inhibition studies showed that the extracts produced curves of similar slope, suggesting conservation of allergenic epitopes. SDS-PAGE revealed differences in protein profiles because roasted extracts generally possessed the same number and proportion of major protein bands but raw extracts varied more in both respects. SDS-PAGE and immunoblotting showed that two of the extracts contained major IgE-binding protein bands that did not appear in the others. One roasted extract gave little protein banding and consequently little IgE binding. Conclusions: Skin testing results showed no differences in the ability of the extracts to provoke a positive skin test response in peanut-sensitive subjects. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. UNIV WISCONSIN,DEPT MED,MADISON,WI. ARKANSAS CHILDRENS HOSP,DEPT PEDIAT IMMUNOL & ALLERGY,LITTLE ROCK,AR 72202. NR 18 TC 31 Z9 31 U1 1 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD APR PY 1995 VL 95 IS 4 BP 837 EP 842 DI 10.1016/S0091-6749(95)70127-3 PG 6 WC Allergy; Immunology SC Allergy; Immunology GA QT468 UT WOS:A1995QT46800009 PM 7722164 ER PT J AU REDDY, SV HUNDLEY, JE WINDLE, JJ ALCANTARA, O LINN, R LEACH, RJ BOLDT, DH ROODMAN, GD AF REDDY, SV HUNDLEY, JE WINDLE, JJ ALCANTARA, O LINN, R LEACH, RJ BOLDT, DH ROODMAN, GD TI CHARACTERIZATION OF THE MOUSE TARTRATE-RESISTANT ACID-PHOSPHATASE (TRAP) GENE PROMOTER SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article ID HUMAN OSTEOCLASTOMAS; PURIFICATION; CLONING; TYPE-5; BONE; UTEROFERRIN; INHIBITION; EXPRESSION; SEQUENCE; REGION AB Tartrate-resistant acid phosphatase (TRAP) is an iron-binding protein that is highly expressed in osteoclasts. To characterize the regulation of TRAP gene expression, progressive 5' and 3' deletions of a 1.8 kb fragment containing the 5'-flanking sequence were fused to a luciferase reporter gene. Two nonoverlapping regions of this 1.8 kb fragment had promoter activity. The upstream promoter (P-1) was located within the region from -881 bp to -463 bp relative to the ATG, while the downstream promoter (P-2) was located between -363 bp to -1 bp in a region we have previously shown to be an intron in transcripts originating from the upstream promoter. A putative repressor region for the P-2 promoter at -1846 bp to -1240 bp and a putative enhancer region at -962 bp to -881 bp relative to the ATG were identified. PCR analysis of promoter-specific transcription of the TRAP gene in various murine tissues showed that both promoters were active in several tissues. Transferrin-bound iron increased P-1 promoter activity 2.5-fold and hemin decreased P-1 promoter activity, but neither had any effect on P-2 activity. These data show that the transcriptional regulation of the TRAP gene is complex and that iron may play a key role in TRAP gene regulation. C1 AUDIE L MURPHY MEM VET ADM MED CTR,HLTH SCI CTR,RES SERV 151,SAN ANTONIO,TX 78284. UNIV TEXAS,DEPT MED HEMATOL,SAN ANTONIO,TX. UNIV TEXAS,DEPT CELL & STRUCT BIOL,SAN ANTONIO,TX 78284. CANC THERAPY & RES CTR S TEXAS,SAN ANTONIO,TX. OI Windle, Jolene/0000-0001-6690-385X FU NIADDK NIH HHS [AM35188]; NIAMS NIH HHS [AR41336, AR39539] NR 22 TC 67 Z9 68 U1 0 U2 2 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD APR PY 1995 VL 10 IS 4 BP 601 EP 606 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QQ479 UT WOS:A1995QQ47900012 PM 7610931 ER PT J AU BELL, NH AF BELL, NH TI VITAMIN-D METABOLISM, AGING, AND BONE LOSS SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Editorial Material ID SENILE OSTEOPOROSIS; FRACTURES; WOMEN; AGE; DEFICIENCY; CALCIUM C1 MED UNIV S CAROLINA, RALPH H JOHNSON VET AFFAIRS MED CTR, DEPT PHARMACOL, CHARLESTON, SC 29425 USA. RP BELL, NH (reprint author), MED UNIV S CAROLINA, RALPH H JOHNSON VET AFFAIRS MED CTR, DEPT MED, CHARLESTON, SC 29425 USA. NR 14 TC 21 Z9 22 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD APR PY 1995 VL 80 IS 4 BP 1051 EP 1051 DI 10.1210/jc.80.4.1051 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QR243 UT WOS:A1995QR24300002 PM 7714064 ER PT J AU TRIAL, JA BIRDSALL, HH HALLUM, JA CRANE, ML RODRIGUEZBARRADAS, MC DEJONG, AL KRISHNAN, B LACKE, CE FIGDOR, CG ROSSEN, RD AF TRIAL, JA BIRDSALL, HH HALLUM, JA CRANE, ML RODRIGUEZBARRADAS, MC DEJONG, AL KRISHNAN, B LACKE, CE FIGDOR, CG ROSSEN, RD TI PHENOTYPIC AND FUNCTIONAL-CHANGES IN PERIPHERAL-BLOOD MONOCYTES DURING PROGRESSION OF HUMAN-IMMUNODEFICIENCY-VIRUS INFECTION - EFFECTS OF SOLUBLE IMMUNE-COMPLEXES, CYTOKINES, SUBCELLULAR PARTICULATES FROM APOPTOTIC CELLS, AND HIV-1-ENCODED PROTEINS ON MONOCYTE PHAGOCYTIC FUNCTION, OXIDATIVE BURST, TRANSENDOTHELIAL MIGRATION, AND CELL-SURFACE PHENOTYPE SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE HIV; MONOCYTE; PHAGOCYTOSIS; ANTIGEN-ANTIBODY COMPLEX; APOPTOSIS ID STAPHYLOCOCCUS-AUREUS; ENVELOPE GLYCOPROTEIN; MONOCLONAL-ANTIBODY; ADHESION MOLECULE; TYPE-1 INFECTION; HIV-INFECTION; FC-RECEPTORS; T-CELLS; AIDS; MACROPHAGES AB We postulated that changes in the cell surface display of molecules that facilitate cell-cell and cell-matrix adhesions may reflect the changing immunosurveillance capacity of blood monocytes during progression of human immunodeficiency virus (HIV) infections, In Centers for Disease Control (CDC) stage A patients, whose monocytes' ability to phagocytose bacteria and generate reactive oxygen intermediates is often increased, the frequency of monocytes expressing CD49d, HLA-DP, HLA-DQ, and an activation epitope of CD11a/CD18 was increased and monocyte transendothelial migration was unimpaired, In CDC stage B/C patients, whose monocytes' ability to phagocytose bacteria and migrate across confluent endothelial monolayers was diminished, surface expression of CD49e and CD62L and the percentage of monocytes expressing CD18, CD11a, CD29, CD49e, CD54, CD58, CD31, and HLA-I were significantly decreased. Incubating normal donor monocytes with immune complexes in vitro reproduced the phenotypic and functional abnormalities seen in stage B/C patients, By contrast, in vitro stimulation with subcellular particulates released by apoptotic lymphocytes reproduced changes seen in stage A patients' monocytes, Although circulating monocytes appear to be activated at all stages, these data suggest that the high levels of circulating immune complexes, found predominantly in the later stages of HIV infection, may be particularly instrumental in reducing the monocyte's capacity to maintain surveillance against infection. C1 HOUSTON VET AFFAIRS MED CTR,IMMUNOL RES LAB,HOUSTON,TX 77030. HOUSTON VET AFFAIRS MED CTR,LAB SERV,HOUSTON,TX 77030. NETHERLANDS CANC INST,AMSTERDAM,NETHERLANDS. BAYLOR COLL MED,DEPT OTORHINOLARYNGOL,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MICROBIOL & IMMUNOL,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT PATHOL,HOUSTON,TX 77030. GREENVILLE HOSP SYST,DIV MED EDUC & RES,GREENVILLE,NC 27835. RP TRIAL, JA (reprint author), HOUSTON VET AFFAIRS MED CTR,AIDS & HIV RELATED INFECT RES CTR,BLDG 109,RM 147,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. RI Figdor, Carl/A-4232-2010 FU NIAID NIH HHS [R01 AI28071, UO1 AI33236]; NINDS NIH HHS [R01 NS32583] NR 54 TC 46 Z9 46 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD APR PY 1995 VL 95 IS 4 BP 1690 EP 1701 DI 10.1172/JCI117845 PG 12 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA QQ596 UT WOS:A1995QQ59600036 PM 7706478 ER PT J AU ASTIN, MC OGLANDHAND, SM COLEMAN, EM FOY, DW AF ASTIN, MC OGLANDHAND, SM COLEMAN, EM FOY, DW TI POSTTRAUMATIC-STRESS-DISORDER AND CHILDHOOD ABUSE IN BATTERED WOMEN - COMPARISONS WITH MARITALLY DISTRESSED WOMEN SO JOURNAL OF CONSULTING AND CLINICAL PSYCHOLOGY LA English DT Note ID VIOLENCE AB In the present study, posttraumatic stress disorder (PTSD) prevalence rates were compared among 50 battered women and 37 maritally distressed women who had not experienced battering (N = 87). Participants were administered R. Spitzer and I. B. S. Williams's (1985) Structured Clinical Interview for the DSM-III-R (Diagnostic and Statistical Manual of Mental Disorders [3rd ed., rev.]) to assess PTSD status and previous traumatic experiences in addition to other standardized measures of PTSD and violence exposure. Battered women exhibited significantly higher rates of PTSD than maritally distressed women (58% vs. 18.9%). Although both groups had similar rates of previous trauma experiences, women with a PTSD-positive status(both battered women and maritally distressed women) were significantly more likely to have experienced self-reported childhood sexual abuse and a higher overall number of previous traumas than those with a PTSD-negative status. Battering exposure and childhood sexual abuse predicted 37% of the variance in overall PTSD intensity levels. C1 FULLER GRAD SCH PSYCHOL,PASADENA,CA. PEPPERDINE UNIV,GRAD SCH EDUC PSYCHOL,MALIBU,CA 90265. W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,LOS ANGELES,CA. NR 16 TC 100 Z9 101 U1 2 U2 2 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0022-006X J9 J CONSULT CLIN PSYCH JI J. Consult. Clin. Psychol. PD APR PY 1995 VL 63 IS 2 BP 308 EP 312 DI 10.1037//0022-006X.63.2.308 PG 5 WC Psychology, Clinical SC Psychology GA QR238 UT WOS:A1995QR23800016 PM 7751492 ER PT J AU LEAF, DA NEIGHBOR, WE SCHAAD, D SCOTT, CS AF LEAF, DA NEIGHBOR, WE SCHAAD, D SCOTT, CS TI A COMPARISON OF SELF-REPORT AND CHART AUDIT IN STUDYING RESIDENT PHYSICIAN ASSESSMENT OF CARDIAC RISK-FACTORS SO JOURNAL OF GENERAL INTERNAL MEDICINE LA English DT Article DE RESIDENTS; PERFORMANCE ASSESSMENT; CARDIAC RISK FACTORS; PHYSICIAN PERCEPTION; CHART AUDIT AB OBJECTIVE: To examine the relationship between resident physicians' perceptions of their preventive cardiology practices and a chart audit assessment of their documented services. DESIGN: A criterion standard comparison of two methods used to assess resident physicians' practices: self-report and chart audit. SETTING: Physician ambulatory care in a residency program. PATIENTS AND OTHER PARTICIPANTS: Coronary artery disease (CAD) risk factor assessment was evaluated by self-report for 72 resident physicians and by chart audit of randomly selected records of 544 of their patients who did not have CAD or a debilitating chronic disease during a one-year period. INTERVENTION: Measurements of the residents' perceived CAD risk factor assessment practice by self-report, and chart audit assessments of their recorded care. MAIN OUTCOME: The relationship between self-reported and chart audit assessments of CAD risk factors. RESULTS: Chart audit assessment of CAD risk factor management was highly significantly (p < 0.01) lower than self-reported behaviors for evaluation of cigarette smoking, diet, physical activity, stress, plasma cholesterol, blood pressure, and body weight/obesity. CONCLUSIONS: Three different interpretations of these findings are apparent. 1) Physician self-report is a poor tool for the measurement of clinical behavior, and therefore research of physician behavior should not rely solely on self-reported data; 2) physicians' chart recording of their clinical practice is insufficient to reflect actual care; or 3) neither is an accurate measure of actual practice. RP LEAF, DA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,DIV GEN INTERNAL MED 691111G,WILSHIRE & SAWTELLE BLVD,LOS ANGELES,CA 90073, USA. FU NHLBI NIH HHS [HL 30564] NR 0 TC 39 Z9 39 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-8734 J9 J GEN INTERN MED JI J. Gen. Intern. Med. PD APR PY 1995 VL 10 IS 4 BP 194 EP 198 DI 10.1007/BF02600254 PG 5 WC Health Care Sciences & Services; Medicine, General & Internal SC Health Care Sciences & Services; General & Internal Medicine GA QT151 UT WOS:A1995QT15100003 PM 7790980 ER PT J AU MATTAMMAL, MB STRONG, R LAKSHMI, VM CHUNG, HD STEPHENSON, AH AF MATTAMMAL, MB STRONG, R LAKSHMI, VM CHUNG, HD STEPHENSON, AH TI PROSTAGLANDIN-H SYNTHETASE-MEDIATED METABOLISM OF DOPAMINE - IMPLICATION FOR PARKINSONS-DISEASE SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE ARACHIDONIC ACID; PROSTAGLANDINS; DOPAMINE; COOXIDATION; NEURONS; PARKINSONS DISEASE ID CARCINOGEN-DNA ADDUCTS; OXYGEN FREE-RADICALS; ACUTE LUNG INJURY; LIPID-PEROXIDATION; SUBSTANTIA-NIGRA; ARACHIDONIC-ACID; HUMAN-BRAIN; OXIDATION; SYNTHASE; ACTIVATION AB Differences in prostaglandin H synthetase (PHS) activity in the substantia nigra of age- and postmortem interval-matched parkinsonian, Alzheimer's, and normal control brain tissue were assessed. Prostaglandin E(2) (PGE(2), an index of PHS activity) was higher in substantia nigra of parkinsonian brain tissue than Alzheimer's or control tissue. Incubation of substantia nigra slices with arachidonic acid (AA) increased PGE(2) synthesis. Dopamine stimulated PHS synthesis of PGE(2). [H-3]Dopamine was activated by PHS to electrophilic intermediate(s) that covalently bound to DNA, microtubulin protein, bovine serum albumin, and sulfhydryl reagents. When AA was replaced by hydrogen peroxide, PHS/H2O2-supported binding proceeded at rates similar to those observed with PHS/AA. Indomethacin and aspirin inhibited AA-mediated cooxidation of dopamine but not H2O2-mediated metabolism. PHS-mediated metabolism of dopamine was not affected by monoamine oxidase inhibitors. Substrate requirements and effects of specific inhibitors suggest cooxidation of dopamine is mediated by the hydroperoxidase activity of PHS. P-32-postlabeling was used to detect dopamine-DNA adducts. PHS/AA activation of dopamine in the presence of DNA resulted in the formation of five dopamine-DNA adducts, i.e., 23, 43, 114, 70, and 270 amol/mu g DNA. DNA adduct formation was PHS, AA, and dopamine dependent. PHS catalyzed cooxidation of dopamine in dopaminergic neuronal degeneration is discussed. C1 VET ADM MED CTR, CLIN LAB SERV, ST LOUIS, MO 63125 USA. ST LOUIS UNIV, CTR HLTH SCI, DIV GERIATR MED, ST LOUIS, MO 63103 USA. ST LOUIS UNIV, CTR HLTH SCI, DIV PATHOL, ST LOUIS, MO 63103 USA. ST LOUIS UNIV, CTR HLTH SCI, DIV PHARMACOL & PHYSIOL SCI, ST LOUIS, MO 63103 USA. AUDIE L MURPHY MEM VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT CELLULAR & STRUCT BIOL, SAN ANTONIO, TX USA. UNIV TEXAS, HLTH SCI CTR, DEPT PHARMACOL, SAN ANTONIO, TX 78284 USA. RP VET ADM MED CTR, GRECC 11G, JEFFERSON BARRACKS, ST LOUIS, MO 63125 USA. FU NCRR NIH HHS [RR-00954]; NIA NIH HHS [AG09557]; NIADDK NIH HHS [AM-20579] NR 53 TC 114 Z9 117 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-3042 EI 1471-4159 J9 J NEUROCHEM JI J. Neurochem. PD APR PY 1995 VL 64 IS 4 BP 1645 EP 1654 PG 10 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA QN150 UT WOS:A1995QN15000026 PM 7891092 ER PT J AU FRUEH, BC LEVERETT, JP KINDER, BN AF FRUEH, BC LEVERETT, JP KINDER, BN TI INTERRELATIONSHIP BETWEEN MMPI-2 AND RORSCHACH VARIABLES IN A SAMPLE OF VIETNAM VETERANS WITH PTSD SO JOURNAL OF PERSONALITY ASSESSMENT LA English DT Article ID POSTTRAUMATIC-STRESS-DISORDER; COMBAT VETERANS; SYMPTOMS AB We examined interrelationships between theoretically related MMPI-2 and Rorschach variables in a sample of Veterans Affairs outpatients with Posttraumatic Stress Disorder (PTSD). Subjects were 20 White Vietnam combat veterans diagnosed with PTSD who completed the Rorschach and MMPI-2 as part of a comprehensive evaluation. Correlations were calculated for variables in three groups: validity, depression and anxiety, and thought disturbance. Results showed strong relationships between m, MOR, and the Dramatic special score of the Rorschach and MMPI-2 indices of distress. Positive relationships were also found for some indicators of thought disturbance, whereas correlations for other depressive indicators were not significant. Findings are discussed with regard to implications for the clinical assessment of combat-related PTSD and future directions for assessment research. C1 UNIV S FLORIDA,TAMPA,FL 33620. RP FRUEH, BC (reprint author), MED UNIV S CAROLINA,RALPH H JOHNSON VET AFFAIRS MED CTR,DEPT PSYCHIAT & BEHAV SCI,CHARLESTON,SC 29401, USA. NR 19 TC 5 Z9 5 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 0022-3891 J9 J PERS ASSESS JI J. Pers. Assess. PD APR PY 1995 VL 64 IS 2 BP 312 EP 318 DI 10.1207/s15327752jpa6402_10 PG 7 WC Psychology, Clinical; Psychology, Social SC Psychology GA QP364 UT WOS:A1995QP36400010 PM 7722856 ER PT J AU WILLIAMS, JW HOLLEMAN, DR SIMEL, DL AF WILLIAMS, JW HOLLEMAN, DR SIMEL, DL TI MEASURING SHOULDER FUNCTION WITH THE SHOULDER PAIN AND DISABILITY INDEX SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE HEALTH STATUS MEASURE; FUNCTIONAL STATUS; QUALITY OF LIFE; SHOULDER FUNCTION ID HEALTH ASSESSMENT QUESTIONNAIRE; DOUBLE-BLIND; RESPONSIVENESS; PHYSIOTHERAPY; INSTRUMENTS; INJECTIONS; ARTHRITIS; VALIDITY; THERAPY; TRIAL AB Objective. To extend the validity of the Shoulder Pain and Disability Index (SPADI) by (1) making it suitable for telephone administration; (2) determining its convergent validity with other health status measures; and (3) assessing the responsiveness of the SPADI to clinical change. Methods, Consecutive primary care patients with shoulder discomfort were followed for 3 months. At enrollment, a detailed shoulder specific history was obtained by a trained research assistant, and the Health Assessment Questionnaire (HAQ), the Medical Outcomes Study SF-20 (SF-20), and numeric and visual analog versions of the SPADI were completed by the patient. At 2, 4, and 12 weeks the numeric scaled SPADI was administered by telephone and patients rated globally the change in shoulder discomfort. Results, One hundred and two subjects were enrolled; 96 completed at least one followup assessment and 75 completed all followup assessments. Subjects were men (98%), predominantly white (73%), with a median age of 60 years, and the majority had experienced shoulder discomfort for >3 months (66%). At baseline the visual analog (VAS) and numeric scaled SPADI were highly concordant (intraclass correlation coefficient = 0.86), and the SPADI correlated substantially with the HAQ (r = 0.61) and the physical functioning (r = -0.50) and pain (r = -0.43) domains of the SF-20, The SPADI(triangle) (baseline - followup) discriminated accurately between subjects who improved versus those who stayed the same or worsened [receiver operating characteristic cure, (ROC) = 0.91, likelihood ratio for improvement = 34]. Conclusion. The numerically scaled SPADI is highly correlated with the original VAS version of the SPADI and other measures of health status. The SPADI is responsive to change and accurately discriminates among patients who are improved or worsened. C1 UNIV TEXAS,HLTH SCI CTR,DIV GEN INTERNAL MED,SAN ANTONIO,TX 78284. UNIV KENTUCKY,DIV MED GENET,MED SERV,LEXINGTON,KY 40506. VET ADM MED CTR,CTR AMBULATORY CARE,LEXINGTON,KY 40511. VET ADM MED CTR,CTR HLTH SERV RES PRIMARY CARE,DURHAM,NC 27705. DUKE UNIV,MED CTR,DIV GEN INTERNAL MED,DURHAM,NC 27710. UNIV TEXAS,AUDIE L MURPHY MEM VET HOSP,HLTH SCI CTR,DIIV GEN INTERNAL MED,AMBULATORY CARE SERV,SAN ANTONIO,TX 78285. RI Williams, Jr., John/A-3696-2008 OI Williams, Jr., John/0000-0002-5267-5558 NR 33 TC 154 Z9 159 U1 2 U2 6 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD APR PY 1995 VL 22 IS 4 BP 727 EP 732 PG 6 WC Rheumatology SC Rheumatology GA QU120 UT WOS:A1995QU12000029 PM 7791172 ER PT J AU MAHONEY, J AF MAHONEY, J TI SCREENING FOR DEPRESSION - REPLY SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Letter C1 UNIV WISCONSIN,MADISON,WI. RP MAHONEY, J (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,GRECC,MADISON,WI, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 1995 VL 43 IS 4 BP 457 EP 457 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QR082 UT WOS:A1995QR08200029 ER PT J AU LANDAU, WM DAUBE, JR AMINOFF, MJ BREY, RL BROOKS, BR DEUEL, RK GALABURDA, AM PORTER, JA ROSENBAUM, RB WHITHAM, RH WIGGS, JW AF LANDAU, WM DAUBE, JR AMINOFF, MJ BREY, RL BROOKS, BR DEUEL, RK GALABURDA, AM PORTER, JA ROSENBAUM, RB WHITHAM, RH WIGGS, JW TI NEUROREALITY .1. DEDICATED DEMOLITION OF THE DECADE OF THE BRAIN - THE GENUINE THREAT TO NEUROLOGIC RESEARCH FROM THE ANIMAL RADICAL RIGHT SO NEUROLOGY LA English DT Editorial Material C1 WASHINGTON UNIV,SCH MED,DEPT NEUROL,ST LOUIS,MO 63110. WASHINGTON UNIV,SCH MED,DEPT PEDIAT,ST LOUIS,MO 63110. MAYO CLIN & MAYO FDN,DEPT NEUROL,ROCHESTER,MN 55905. UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV WISCONSIN HOSP & CLIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,NEUROL SERV,MADISON,WI 53792. BETH ISRAEL HOSP,BEHAV NEUROL UNIT,BOSTON,MA 02215. OREGON HLTH SCI UNIV,DEPT NEUROL,PORTLAND,OR 97201. NR 0 TC 4 Z9 4 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD APR PY 1995 VL 45 IS 4 BP 609 EP 610 PG 2 WC Clinical Neurology SC Neurosciences & Neurology GA QT454 UT WOS:A1995QT45400002 PM 7723943 ER PT J AU SCHOEMAN, RJ ROSE, DL AF SCHOEMAN, RJ ROSE, DL TI DESTRUCTIVE GINGIVAL MASS SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Discussion C1 UNIV MINNESOTA,SCH DENT,DEPT ENDODONT,MINNEAPOLIS,MN 55455. UNIV CALIF LOS ANGELES,CTR HLTH SCI,SCH DENT,ORAL & MAXILLOFACIAL SURG SECT,LOS ANGELES,CA 90024. RP SCHOEMAN, RJ (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 9 TC 3 Z9 3 U1 0 U2 0 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD APR PY 1995 VL 79 IS 4 BP 407 EP 409 DI 10.1016/S1079-2104(05)80118-0 PG 3 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA QT453 UT WOS:A1995QT45300005 PM 7614196 ER PT J AU TAKAHASHI, LK KIM, H AF TAKAHASHI, LK KIM, H TI RELATIVE CONTRIBUTIONS OF PITUITARY-ADRENAL HORMONES TO THE ONTOGENY OF BEHAVIORAL-INHIBITION IN THE RAT SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE PREWEANLING RATS; BEHAVIORAL INHIBITION; FREEZING; ULTRASONIC VOCALIZATIONS; HYPOPHYSECTOMY; ADRENALECTOMY; ACTH; CORTICOSTERONE ID PREWEANLING RATS; BRAIN-DEVELOPMENT; BETA-ENDORPHIN; CORTICOTROPIN; CORTICOSTERONE; SECRETION; RESPONSES AB Recent investigations revealed that adrenalectomized (ADX) rat pups exhibit deficits in behavioral inhibition. Furthermore, administration of exogenous corticosterone (CORT) restores behavioral inhibition in ADX pups. Although these studies suggest that CORT has an important role in the development of behavioral inhibition, the relative behavioral effects of elevated pituitary hormone secretion induced by ADX are not known. Therefore, experiments were conducted to assess the potential behavioral effects of elevated adrenocorticotropin (ACTH) secretion induced by ADX and to further evaluate the contribution of endogenous CORT to the development of behavioral inhibition. In Experiment 1., we verified that 10-day-old ADX rats exhibit high levels of plasma ACTH throughout the preweaning period associated with the development of behavioral inhibition. In Experiment 2, 10-day-old pups were hypophysectomized (HYPOX) and ADX acid were compared behaviorally to sham-operated controls on day 14. When tested in the presence of an anesthetized unfamiliar adult male rat, HYPOX + ADX pups exhibited low levels of freezing accompanied by ultrasonic vocalizations. These pups also had reduced concentrations of plasma ACTH and CORT. In Experiment 3, 10-day-old pups were HYPOX and tested for behavioral inhibition on day 14. In comparison to sham-operated controls, HYPOX rats exhibited significantly lower levels of freezing and had reduced plasma concentrations of ACTH and CORT. Results demonstrate clearly that deficits in freezing occur even in the presence of low plasma ACTH concentrations. Therefore, elevated secretion of pituitary hormones is not a major factor that contributes to the ADX-induced deficits in behavioral inhibition. In addition, results considerably strengthen the contention that endogenous adrenal steroids have a prominent role in the development of neural pathways regulating the expression of behavioral inhibition. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53792. RP TAKAHASHI, LK (reprint author), UNIV WISCONSIN,SCH MED,DEPT PSYCHIAT,600 HIGHLAND AVE,MADISON,WI 53792, USA. FU NIMH NIH HHS [MH-43986] NR 36 TC 11 Z9 11 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD APR PY 1995 VL 57 IS 4 BP 711 EP 716 DI 10.1016/0031-9384(94)00324-6 PG 6 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA QL348 UT WOS:A1995QL34800015 PM 7777608 ER PT J AU SCHUMACHER, HR AF SCHUMACHER, HR TI ARTHRITIS OF RECENT-ONSET - A GUIDE TO EVALUATION AND INITIAL THERAPY FOR PRIMARY-CARE PHYSICIANS SO POSTGRADUATE MEDICINE LA English DT Article AB Correct early diagnosis of acute and recent-onset arthritis is important to prognosis. The erosive damage done by rheumatoid arthritis occurs earlier in the disease course than previously realized, and more specific therapies to minimize damage are becoming available, Also, arthritis may be the initial clue to a serious systemic disease. Determining whether one, several, or many joints are affected can narrow the diagnostic possibilities, Arthrocentesis and synovial fluid testing provide much information and should be done at initial evaluation if possible. The presence or absence of fever, rash, family history of joint disease, and exposure to infective organisms can further direct diagnostic studies and treatment, In general, to avoid masking clues, drug therapy should be delayed for mild symptoms until diagnosis is complete. C1 UNIV PENN,SCH MED,DIV RHEUMATOL,PHILADELPHIA,PA 19104. RP SCHUMACHER, HR (reprint author), VET AFFAIRS MED CTR,CTR ARTHRITIS IMMUNOL,ARTHRITIS IMMUNOL UNIT,39TH & WOODLAND AVE,PHILADELPHIA,PA 19104, USA. NR 8 TC 2 Z9 2 U1 0 U2 0 PU MCGRAW HILL HEALTHCARE PUBLICATIONS PI MINNEAPOLIS PA 4530 WEST 77TH ST, MINNEAPOLIS, MN 55435-5000 SN 0032-5481 J9 POSTGRAD MED JI Postgrad. Med. PD APR PY 1995 VL 97 IS 4 BP 52 EP & PG 0 WC Medicine, General & Internal SC General & Internal Medicine GA QR457 UT WOS:A1995QR45700009 PM 7716092 ER PT J AU SILVA, JA LEONG, GB AF SILVA, JA LEONG, GB TI STUDYING VIOLENT BEHAVIOR SO PSYCHIATRIC SERVICES LA English DT Letter C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. VET AFFAIRS MED CTR,SAN ANTONIO,TX. VET AFFAIRS MED CTR,LOS ANGELES,CA. RP SILVA, JA (reprint author), UNIV TEXAS,HLTH SCI CTR,7703 FLOYD CURL DR,SAN ANTONIO,TX, USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 1075-2730 J9 PSYCHIATR SERV JI Psychiatr. Serv. PD APR PY 1995 VL 46 IS 4 BP 408 EP 408 PG 1 WC Health Policy & Services; Public, Environmental & Occupational Health; Psychiatry SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Psychiatry GA QP561 UT WOS:A1995QP56100022 PM 7788469 ER PT J AU SCHERER, RF CANTY, AL PETERSON, FL COOPER, RF AF SCHERER, RF CANTY, AL PETERSON, FL COOPER, RF TI IDENTIFICATION OF MANAGERIAL BEHAVIOR DIMENSIONS IN A FEDERAL HEALTH-CARE AGENCY SO PSYCHOLOGICAL REPORTS LA English DT Article AB Understanding the behavior of managers provides an opportunity to assess congruencies between organizational needs and managerial skills. This assessment is critical in federal health-care wherein the environment is rapidly changing. In the current investigation, dimensions of managerial behavior for 267 managers in a federal health-care agency were identified. Recommendations are provided with respect to the relevance of using these dimensions for organizational training and development activities. C1 UNIV MISSISSIPPI,UNIVERSITY,MS 38677. US DEPT VET AFFAIRS,DAYTON,OH. RP SCHERER, RF (reprint author), WRIGHT STATE UNIV,COLL BUSINESS & ADM,DAYTON,OH 45435, USA. NR 9 TC 2 Z9 2 U1 1 U2 2 PU PSYCHOLOGICAL REPORTS PI MISSOULA PA P O BOX 9229, MISSOULA, MT 59807 SN 0033-2941 J9 PSYCHOL REP JI Psychol. Rep. PD APR PY 1995 VL 76 IS 2 BP 675 EP 679 PG 5 WC Psychology, Multidisciplinary SC Psychology GA RB714 UT WOS:A1995RB71400061 PM 7667482 ER PT J AU AMES, D CARTER, J WIRSHING, WC MARDER, SR GOLDSTEIN, MJ AF AMES, D CARTER, J WIRSHING, WC MARDER, SR GOLDSTEIN, MJ TI CLOZAPINE ASSOCIATED EOSINOPHILIA AND NEUTROPENIA SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 141 EP 142 DI 10.1016/0920-9964(95)95439-G PG 2 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200415 ER PT J AU MARDER, SR AF MARDER, SR TI INTEGRATING PSYCHOSOCIAL AND PHARMACOLOGICAL STRATEGIES FOR MAINTENANCE THERAPY SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 159 EP 159 DI 10.1016/0920-9964(95)95494-T PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200468 ER PT J AU AMES, D BARTZOKIS, G PIERRE, J SUN, A BERISFORD, MA MARDER, SR WIRSHING, WC AF AMES, D BARTZOKIS, G PIERRE, J SUN, A BERISFORD, MA MARDER, SR WIRSHING, WC TI THE RELATIONSHIP OF SERUM IRON INDEXES TO AKATHISIA, TARDIVE-DYSKINESIA, AND PLASMA PROLIXIN LEVELS SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RI Bartzokis, George/K-2409-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1995 VL 15 IS 1-2 BP 212 EP 212 DI 10.1016/0920-9964(95)95650-X PG 1 WC Psychiatry SC Psychiatry GA QN952 UT WOS:A1995QN95200626 ER PT J AU YOKOZAWA, T MIYAUCHI, A KUMA, K SUGAWARA, M AF YOKOZAWA, T MIYAUCHI, A KUMA, K SUGAWARA, M TI ACCURATE AND SIMPLE METHOD OF DIAGNOSING THYROID-NODULES BY THE MODIFIED TECHNIQUE OF ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION BIOPSY SO THYROID LA English DT Article ID MANAGEMENT; GUIDANCE AB We describe an accurate and simple method of diagnosing thyroid nodules by the modified technique of ultrasound-guided fine needle aspiration biopsy (UG-FNAB). An Aloka SSD 2000 focus type scanner (Aloka Co., Tokyo, Japan) equipped with a 10-MHz mechanical sector probe and a 7.5-MHz convex probe was used. First, a clear picture of the thyroid gland was taken with the use of the 10-MHz probe to determine the abnormalities. Then, a 7.5-MHz probe (1.5 x 0.8 cm of surface area) was used to guide the needle perpendicularly to the neck. This method enabled us to obtain samples from nodules greater than 5 mm. We applied this technique to 1000 patients who had uncertain diagnosis by the conventional method of FNAB. The advantages of this method are as follows: (i) The use of a small probe (7.5 MHz) is most important, since a needle can be inserted by watching the monitor without an assistant. (ii) The biopsy site can be chosen precisely. (iii) Small thyroid cancers, i.e., 5 mm cancer, can be biopsied without any difficulty. (iv) A life-threatening anaplastic carcinoma can be diagnosed at an early stage. (v) An intraglandular metastasis to the opposite lobe can be detected. (vi) Cystic carcinoma, difficult to diagnose by FNAB, can be diagnosed accurately. This method is highly recommended for all clinicians who are currently doing FNAB. C1 KAGAWA MED SCH,DEPT SURG 2,TAKAMATSU,KAGAWA,JAPAN. W LOS ANGELES VET AFFAIRS MED CTR,DIV ENDOCRINOL & METAB,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90073. NR 9 TC 111 Z9 112 U1 1 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 SN 1050-7256 J9 THYROID JI Thyroid PD APR PY 1995 VL 5 IS 2 BP 141 EP 145 DI 10.1089/thy.1995.5.141 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QZ364 UT WOS:A1995QZ36400012 PM 7647575 ER PT J AU ETCHASON, J PETZ, L KEELER, E CALHOUN, L KLEINMAN, S SNIDER, C FINK, A BROOK, R AF ETCHASON, J PETZ, L KEELER, E CALHOUN, L KLEINMAN, S SNIDER, C FINK, A BROOK, R TI THE COST-EFFECTIVENESS OF PREOPERATIVE AUTOLOGOUS BLOOD DONATIONS SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; TRANSFUSION-INDUCED IMMUNOMODULATION; QUALITY-OF-LIFE; NON-A-HEPATITIS; NON-B-HEPATITIS; INFECTION; HEALTH; REPLACEMENT; COLLECTION; COMPONENTS AB Background. Since the recognition that human immunodeficiency virus is transmissible by blood transfusion there has been increasing public and professional support for autologous blood donations before elective surgery. Autologous blood donation is, however, a more expensive process than the donation of allogeneic blood by community volunteers, Furthermore, there have been recent improvements in the safety of the volunteer blood supply, Methods. We used a decision-analysis model to assess the cost effectiveness of donating autologous blood for four surgical procedures, Cost data were collected from the observation of transfusion practice at the University of California, Los Angeles, in 1992, Estimates of the risks of transfusion-associated diseases and the costs of treating them came from the medical literature. Cost effectiveness was expressed in dollars per quality-adjusted year of life saved. We performed sensitivity analyses of the variables in our model and examined the effect of strategies suggested to reduce costs. Results. Substituting autologous for allogeneic blood resulted in little expected health benefit (0.0002 to 0.00044 quality-adjusted year of life saved) at considerable additional cost ($68 to $4,783 per unit of blood). The additional cost of autologous blood was primarily a function of the discarding of units that were donated but not transfused and of a more labor-intensive donation process, The cost-effectiveness values ranged from $235,000 to over $23 million per quality-adjusted year of life saved. Conclusions. Given the improved safety of allogeneic transfusions today, the increased protection afforded by donating autologous blood is limited and may not justify the increased cost. C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,CTR HLTH SCI,DEPT PATHOL & LAB MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,CTR HLTH SCI,DEPT MED,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,CTR HLTH SCI,DEPT HLTH SERV,LOS ANGELES,CA 90024. RAND CORP,SANTA MONICA,CA. RP ETCHASON, J (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,DIV GEN INTERNAL MED,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. RI Brugnara, Carlo/A-8041-2010 OI Brugnara, Carlo/0000-0001-8192-8713 FU NHLBI NIH HHS [K07 HL02151] NR 46 TC 357 Z9 361 U1 1 U2 4 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 16 PY 1995 VL 332 IS 11 BP 719 EP 724 DI 10.1056/NEJM199503163321106 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA QL788 UT WOS:A1995QL78800006 PM 7854380 ER PT J AU BOND, CA RAEHL, CL PITTERLE, ME AF BOND, CA RAEHL, CL PITTERLE, ME TI COST OF PHARMACEUTICAL SERVICES IN US HOSPITALS IN 1992 SO AMERICAN JOURNAL OF HEALTH-SYSTEM PHARMACY LA English DT Article DE ADMINISTRATION; CLINICAL PHARMACY; COSTS; DATA COLLECTION; DRUG DISTRIBUTION SYSTEMS; ECONOMICS; EDUCATION, PHARMACEUTICAL; GEOGRAPHY; PERSONNEL, PHARMACY; PHARMACEUTICAL SERVICES; PHARMACISTS, HOSPITAL; PHARMACY, INSTITUTIONAL, HOSPITAL; SALARIES; UNITED STATES ID CLINICAL PHARMACY; CASE-MIX; CARE; OUTCOMES; SEVERITY; ILLNESS AB The results of a 1992 national survey of hospital-based pharmaceutical services are reported and compared with data collected during a similar survey in 1989. A questionnaire was mailed to pharmacy directors at all 3756 medical-surgical hospi tals in the United States that had 50 or more licensed beds. Cost results were evaluated both as unadjusted data and as data adjusted for severity of illness with the case mix index. The response rate was 43% (1597 usable responses). Mean +/- S.D. unadjusted medication costs per occupied bed were $9850 +/- 4744 (a 46% increase over 1989 costs); significant differences were observed for geographic region, hospital ownership, drug delivery system, and pharmacy director's education. Mean +/- S.D. unadjusted total pharmacy costs per occupied bed were $16,550 +/- 6,249 (a 40% increase over 1989 costs); significant differences were observed for geographic region, hospital ownership, drug delivery system, and pharmacy director's education. Other mean +/- S.D. unadjusted pharmacy cost components were as follows: injectable solution costs, $2627 +/- 2191 (a 38% increase over 1989 costs); inventory costs, $2029 +/- 2593 (70% increase); pharmacist salary costs per occupied bed, $2997 +/- 1267 (33% increase); pharmacy technician costs per occupied bed, $995 +/- 876 (24% increase); pharmacist salary costs per full-time equivalent (FTE), $43,798 +/- 12,206 (14% increase); pharmacy technician salary costs per FTE, $18,953 +/- 6,154 (15% increase); and pharmacy staff development costs per occupied bed, $45 +/- 41 (29% increase). Pharmacist salary costs associated with centrally based clinical pharmacy services ranged from a high of $361 per occupied bed per year for drug-use evaluation to a low of $15 per occupied bed per year for inservice education. Pharmacist salary costs for patient-specific pharmaceutical services ranged from $3 per patient for medical rounds to $8 per patient for cardiopulmonary resuscitation team participation and drug protocol management. A 1992 survey provided comprehensive data on the cost structure of hospital-based pharmaceutical services and a basis for comparison with 1989 cost data. C1 UNIV WISCONSIN,SCH MED,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,ARRHYTHMIA CLIN,MADISON,WI 53706. RP BOND, CA (reprint author), UNIV WISCONSIN,SCH PHARM,425 N CHARTER ST,MADISON,WI 53706, USA. NR 51 TC 9 Z9 9 U1 0 U2 0 PU AMER SOC HEALTH-SYSTEM PHARMACISTS PI BETHESDA PA 7272 WISCONSIN AVE, BETHESDA, MD 20814 SN 1079-2082 J9 AM J HEALTH-SYST PH JI Am. J. Health-Syst. Pharm. PD MAR 15 PY 1995 VL 52 IS 6 BP 603 EP 613 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QM521 UT WOS:A1995QM52100005 PM 7606574 ER PT J AU HERBERT, V SHAW, S JAYATILLEKE, E AF HERBERT, V SHAW, S JAYATILLEKE, E TI SERUM FERRITIN-IRON (HOLOFERRITIN) - A NEW TEST FOR IRON-DEFICIENCY OR EXCESS SO FASEB JOURNAL LA English DT Meeting Abstract C1 MT SINAI MED CTR,NEW YORK,NY. QUIXOTE ASSOCIATES INC,NEW YORK,NY. BRONX VET ADM MED CTR,NEW YORK,NY. NR 1 TC 2 Z9 2 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A974 EP A974 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40602033 ER PT J AU LIU, K ROMAN, JS KAMALI, V NAUGHTON, BA KELLER, J RUSCETTI, FW ROODMAN, D BONEWALD, L MEAGHER, RM PURCHIO, AF TWARDZIK, DR AF LIU, K ROMAN, JS KAMALI, V NAUGHTON, BA KELLER, J RUSCETTI, FW ROODMAN, D BONEWALD, L MEAGHER, RM PURCHIO, AF TWARDZIK, DR TI STEM-CELL PROLIFERATION FACTOR (SCPF) INDUCES THE EXPANSION OF PRIMITIVE CD34 CELLS IN CULTURE SO FASEB JOURNAL LA English DT Meeting Abstract C1 ADV TISSUE SCI INC,LA JOLLA,CA 92037. NCI,FREDERICK,MD 21702. HOXWORTH BLOOD CTR,CINCINNATI,OH 45267. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A940 EP A940 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40601840 ER PT J AU RODNEY, G ANDRADE, F MAXWELL, L NAPIER, B JERVIS, W WOLYNEIC, W WEGNER, C LEVINE, S ANZUETO, A JENKINSON, S AF RODNEY, G ANDRADE, F MAXWELL, L NAPIER, B JERVIS, W WOLYNEIC, W WEGNER, C LEVINE, S ANZUETO, A JENKINSON, S TI SUPEROXIDE-DISMUTASE (SOD) PROTECTS DIAPHRAGM (DPH) AGAINST IMPAIRMENT BY INSPIRATORY RESISTIVE LOADING (IRL) SO FASEB JOURNAL LA English DT Meeting Abstract C1 BOEHRINGER INGELHEIM KG,INGELHEIM,CT. WILFORD HALL USAF MED CTR,SAN ANTONIO,TX 78236. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RI Andrade, Francisco/F-1258-2011 OI Andrade, Francisco/0000-0002-2460-5798 NR 2 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 10 PY 1995 VL 9 IS 4 BP A652 EP A652 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QM406 UT WOS:A1995QM40600162 ER PT J AU BHANDARI, B WENZEL, UO MARRA, F ABBOUD, HE AF BHANDARI, B WENZEL, UO MARRA, F ABBOUD, HE TI A NUCLEAR-PROTEIN IN MESANGIAL CELLS THAT BINDS TO THE PROMOTER REGION OF THE PLATELET-DERIVED GROWTH FACTOR-A CHAIN GENE - INDUCTION BY PHORBOL ESTER SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID MICROVASCULAR ENDOTHELIAL-CELLS; NF-KAPPA-B; MAJOR HISTOCOMPATIBILITY COMPLEX; GLUTAMINE-SYNTHETASE GENE; VIRUS TYPE-1 ENHANCER; SMOOTH-MUSCLE CELLS; TRANSCRIPTION FACTOR; MESSENGER-RNAS; EXPRESSION; SEQUENCE AB Mesangial cells predominantly express platelet-derived growth factor (PDGF)-A chain mRNA and release PDGF. Mesangial cell PDGF-A chain mRNA abundance is regulated by several agents including phorbol esters. We have recently demonstrated that induction of PDGF-A chain mRNA abundance in response to phorbol 12-myristate 13-acetate is primarily due to gene transcription. We have now analyzed the 5'-flanking region of the PDGF-A chain promoter to identify DNA binding protein(s) which have the potential to regulate PDGF-A chain gene transcription in human mesangial cells. DNase I footprint analysis of the 5'-flanking region of the PDGF-A chain promoter identifies a DNase I protected region at the location -82 to -102 corresponding to the sequence 5'-GGCCCGGAATCCGGGGGAGGC-3'. Therefore, nuclear extracts from human mesangial cells contain a protein, PDGF-A-BP-1, that binds to a DNA sequence (-82 to -102) in the promoter region of the PDGF-A chain gene. Gel mobility shift analysis using labeled oligomer corresponding to the binding site for PDGF-A-BP-1 indicates that PDGF-A-BP-1 is induced by phorbol ester in mesangial cells as well as fat-storing cells (>20 fold). Egr-1. protein does not bind to labeled PDGF-A-BP-1 oligomer and does not compete with the binding of PDGF-A-BP-1. In addition, SP-1 binding sequence does not compete with the binding sequence of the mesangial cell protein. PDGF-A-BP-1 appears to represent a novel protein which is induced by phorbol ester and thus has the potential for an important role in the transcriptional regulation of the PDGF-A chain gene in mesangial cells and other vascular pericytes. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP BHANDARI, B (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV NEPHROL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. RI Marra, Fabio/K-7263-2016 OI Marra, Fabio/0000-0001-8629-0878 FU NIDDK NIH HHS [DK 43988] NR 44 TC 19 Z9 19 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 10 PY 1995 VL 270 IS 10 BP 5541 EP 5548 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA QL580 UT WOS:A1995QL58000089 PM 7890673 ER PT J AU CHRISTODOULIDES, N DURANTE, W SCHAFER, AI AF CHRISTODOULIDES, N DURANTE, W SCHAFER, AI TI REGULATION OF GUANYLYL CYCLASE ACTIVITY BY VASCULAR SMOOTH-MUSCLE CELL-DERIVED CARBON-MONOXIDE SO FASEB JOURNAL LA English DT Meeting Abstract C1 HOUSTON VAMC,HOUSTON,TX 77030. BAYLOR COLL MED,HOUSTON,TX 77030. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A328 EP A328 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98701929 ER PT J AU DURANTE, W LIAO, L IFTIKHAR, I SCHAFER, AI AF DURANTE, W LIAO, L IFTIKHAR, I SCHAFER, AI TI DIFFERENTIAL REGULATION OF L-ARGININE TRANSPORT AND NITRIC-OXIDE PRODUCTION BY CULTURED VASCULAR CELLS SO FASEB JOURNAL LA English DT Meeting Abstract C1 HOUSTON VAMC,HOUSTON,TX 77030. BAYLOR COLL MED,HOUSTON,TX 77030. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A556 EP A556 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703246 ER PT J AU GUO, ZM WU, WT HALEYZITLIN, V RICHARDSON, A AF GUO, ZM WU, WT HALEYZITLIN, V RICHARDSON, A TI THE USE OF CULTURED RAT HEPATOCYTES TO STUDY THE REPAIR OF SPECIFIC GENES SO FASEB JOURNAL LA English DT Meeting Abstract C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A540 EP A540 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703148 ER PT J AU PAHLAVANI, MA HARRIS, MD RICHARDSON, A AF PAHLAVANI, MA HARRIS, MD RICHARDSON, A TI AGE-RELATED-CHANGES IN TRANSCRIPTION FACTOR NFAT IN RAT LYMPHOCYTES SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A534 EP A534 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98703116 ER PT J AU SCREMIN, OU LI, MG AF SCREMIN, OU LI, MG TI SEVERE CORTICAL CONTUSION INDUCES HYPEREMIA IN THE SYMMETRICAL CONTRALATERAL CORTEX SO FASEB JOURNAL LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA 90073. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A381 EP A381 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702232 ER PT J AU WILLIAMS, MD VANREMMEN, H RICHARDSON, A AF WILLIAMS, MD VANREMMEN, H RICHARDSON, A TI CHARACTERIZATION OF THE CATALASE PROMOTER SO FASEB JOURNAL LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DEPT PHYSIOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 9 PY 1995 VL 9 IS 3 BP A396 EP A396 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA QL987 UT WOS:A1995QL98702317 ER PT J AU SINGH, AK GULATI, S AF SINGH, AK GULATI, S TI EFFECT OF ISCHEMIA-REPERFUSION INJURY ON THE MORPHOLOGY OF PEROXISOMES SO MOLECULAR AND CELLULAR BIOCHEMISTRY LA English DT Article DE PEROXISOMES; BIOGENESIS; KIDNEY; ISCHEMIA; REPERFUSION ID RAT-LIVER PEROXISOMES; SUPEROXIDE-DISMUTASE; MYOCARDIAL REPERFUSION; RENAL CIRCULATION; XANTHINE-OXIDASE; FREE-RADICALS; CELL INJURY; KIDNEY; BIOGENESIS; CATALASE AB We have previously demonstrated that ischemic injury changed the density of peroxisomes into two distinct peaks, one with a normal density (1.21 g/cm(3); Peak I) and a second peak with a lighter density (1.14 g/cm(3); Peak II). We studied the peroxisomes from both peaks under the Electron microscope. Examination of peak I following ischemia showed loss of matrix proteins and damaged limiting membranes with leakage of DAB positive material in direct proportion to the duration of ischemia. Upon reperfusion of the ischemic liver Peak I showed more severe damage to the organelle. These observations clearly demonstrated that ischemia reperfusion injury causes structural damage to peroxisomes. Interestingly ultrastructural examination of Peak II following ischemia showed evidence of perisomal proliferation with budding of existing peroxisomes and the presence of micro peroxisomes (changes similar to those noted under conditions leading to perisomal proliferation). However, peak II following reperfusion showed only damaged organelle. These observations underline the importance of peroxisomes in the response of the cell to ischemia-reperfusion injury. C1 MED UNIV S CAROLINA,DEPT PATHOL & LAB MED,CHARLESTON,SC 29425. RP SINGH, AK (reprint author), RALPH H JOHNSON VA MED CTR,DEPT PATHOL & LAB MED,109 BEE ST,CHARLESTON,SC 29401, USA. NR 39 TC 6 Z9 6 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0300-8177 J9 MOL CELL BIOCHEM JI Mol. Cell. Biochem. PD MAR 9 PY 1995 VL 144 IS 1 BP 19 EP 26 DI 10.1007/BF00926736 PG 8 WC Cell Biology SC Cell Biology GA QT315 UT WOS:A1995QT31500004 PM 7791741 ER PT J AU FEIGIN, AM ARONOV, EV TEETER, JH BRAND, JG AF FEIGIN, AM ARONOV, EV TEETER, JH BRAND, JG TI THE PROPERTIES OF ION CHANNELS FORMED BY THE COUMARIN ANTIBIOTIC, NOVOBIOCIN, IN LIPID BILAYERS SO BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES LA English DT Article DE NOVOBIOCIN; ION CHANNEL; HPLC PURIFICATION ID PHOSPHOLIPID SURFACE-CHARGE; AMPHOTERICIN-B CHANNELS; GRAMICIDIN-A; K+ CHANNEL; FROG-SKIN; CONDUCTANCE; MEMBRANES; SELECTIVITY; NYSTATIN; STATES AB The coumarin antibiotic novobiocin forms ion channels of varying conductances in lipid bilayers. The conductances (about 20, 22, 14, 7 and 2 pS for 100 mM NH4Cl, CsCl, KCl, NaCl and LiCl, respectively) and selectivities (cation transference numbers in the range of 0.97-0.98) of one type of novobiocin-induced channel are similar to those found for channels formed by gramicidin A, an antibiotic of very different structure. The conductance of novobiocin channels of this type was independent of the species of the membrane lipid. This observation suggests that novobiocin molecules directly form these channels, and that channels are not formed through defects in lipid structure. The similarity in conductance and ion selectivity between channels induced by novobiocin and those formed by gramicidin A suggests that these structurally different molecules form channels with comparable internal diameter and internal surface charge distribution. Using HPLC purification we argue that the channel-forming activity of novobiocin is related to the activity of the novobiocin molecule itself, and not to a contaminant of the commercially available novobiocin sodium salt preparation. C1 UNIV PENN,PHILADELPHIA,PA 19104. DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP FEIGIN, AM (reprint author), MONELL CHEM SENSES CTR,3500 MARKET ST,PHILADELPHIA,PA 19104, USA. NR 37 TC 5 Z9 5 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0005-2736 J9 BBA-BIOMEMBRANES JI Biochim. Biophys. Acta-Biomembr. PD MAR 8 PY 1995 VL 1234 IS 1 BP 43 EP 51 DI 10.1016/0005-2736(94)00257-P PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA QL423 UT WOS:A1995QL42300007 PM 7533542 ER PT J AU BURT, VK YAGER, J LUNDGREN, J AF BURT, VK YAGER, J LUNDGREN, J TI PROVIDING RESIDENTS WITH A COMPREHENSIVE EDUCATIONAL-PROGRAM IN OUTPATIENT PSYCHIATRY - INTEGRATING AN OUTPATIENT CURRICULUM INTO OUTPATIENT MANAGEMENT TEAMS SO ACADEMIC PSYCHIATRY LA English DT Article ID PROJECTIVE IDENTIFICATION; PSYCHOTHERAPY AB As part of their efforts to prepare psychiatry residents for comprehensive, practical out-patient psychiatric practice, the authors have established an organized training program in ambulatory psychiatry. The program consists of outpatientf management teams that run from mid-PGY-2 to PGY-4, a specified minimum number of mandatory outpatient hours for continuity patient care, and suggested guidelines for residents' outpatient experiences. An outpatient management team curriculum has been designed for team leaders and trainees that consists of specific topics in outpatient care, associated learning objectives, and readings for each topic. This curriculum, which supplements our previous program of conferences, individual supervision, and a yearlong psychotherapy seminar series, has been refined over the past 5 years. The authors describe the program and the topics included in the curriculum. C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT PSYCHIAT,LOS ANGELES,CA. RP BURT, VK (reprint author), UNIV CALIF LOS ANGELES,SCH MED,INST NEUROPSYCHIAT,760 WESTWOOD PLAZA,LOS ANGELES,CA 90024, USA. NR 52 TC 0 Z9 0 U1 1 U2 1 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 1042-9670 J9 ACAD PSYCHIATR JI Acad. Psych. PD SPR PY 1995 VL 19 IS 1 BP 22 EP 33 PG 12 WC Education & Educational Research; Psychiatry SC Education & Educational Research; Psychiatry GA QK312 UT WOS:A1995QK31200004 PM 24435570 ER PT J AU CHIAPPELLI, F TAYLOR, AN AF CHIAPPELLI, F TAYLOR, AN TI THE FETAL ALCOHOL SYNDROME AND FETAL ALCOHOL EFFECTS ON IMMUNE COMPETENCE SO ALCOHOL AND ALCOHOLISM LA English DT Letter ID PROLIFERATIVE RESPONSE; PRENATAL EXPOSURE; ETHANOL INUTERO; RATS; CELLS C1 W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,HUMAN IMMUNOL & PSYCHONEUROIMMUNOL LABS,LOS ANGELES,CA 90073. RP CHIAPPELLI, F (reprint author), UNIV CALIF LOS ANGELES,SCH MED,DEPT ANAT & CELL BIOL,LOS ANGELES,CA 90024, USA. FU NIDA NIH HHS [DA07683] NR 42 TC 16 Z9 16 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0735-0414 J9 ALCOHOL ALCOHOLISM JI Alcohol Alcohol. PD MAR PY 1995 VL 30 IS 2 BP 259 EP 262 PG 4 WC Substance Abuse SC Substance Abuse GA RC015 UT WOS:A1995RC01500016 PM 7662046 ER PT J AU AMMAR, A WONG, M SINGH, BN AF AMMAR, A WONG, M SINGH, BN TI DIVERGENT EFFECTS OF CHRONIC AMIODARONE ADMINISTRATION ON SYSTOLIC AND DIASTOLIC FUNCTION IN PATIENTS WITH HEART-DISEASE SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID VENTRICULAR-FUNCTION; ARRHYTHMIAS; FAILURE AB The purpose of this study was to determine the effects of chronic amiodarone treatment on systolic and diastolic function in patients with cardiac disease undergoing treatment for resistant ventricular arrhythmias. Previous studies have shown that chronic amiodarone treatment either has no effect or increases left ventricular ejection fraction, but the effects on diastolic properties of the ventricle have not been defined. Twelve male patients were given loading doses of amiodarone followed by a maintenance regimen. Serial measurements of heart rate, blood pressure and indexes of systolic and diastolic function were measured by Doppler echocardiographic techniques at baseline conditions and at 2, 8, and 12 weeks of drug therapy. Changes in altered thyroid state were excluded by serial determinations of thyroid function, Amiodarone increased left ventricular ejection fraction (+16%, p <0.01 by 8 weeks), decreased presystolic ejection period/left ventricular ejection time (-12%, p <0.01 by 8 weeks), and increased velocity of circumferential fiber shortening (+22%, p <0.05 by 8 weeks). Amiodarone decreased mitral inflow velocity peak E/peak A (-7%, p <0.01 by 12 weeks), and increased deceleration and isovolumic relaxation times incrementally (+36% [p <0.001] and +23% [p <0.001], respectively, at 12 weeks). Chronically administered amiodarone can improve systolic function and exert a negative lusitropic action in patients with heart disease. C1 W LOS ANGELES VET AFFAIRS MED CTR,MED SERV,CARDIOL SECT W111E,LOS ANGELES,CA 90073. ZAGAZIG UNIV,ZAGAZIG,EGYPT. UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024. NR 27 TC 17 Z9 18 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 1 PY 1995 VL 75 IS 7 BP 465 EP 469 DI 10.1016/S0002-9149(99)80582-7 PG 5 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QH855 UT WOS:A1995QH85500008 PM 7863990 ER PT J AU ROMANGOLDSTEIN, S MITCHELL, P CROSSEN, JR WILLIAMS, PC TINDALL, A NEUWELT, EA AF ROMANGOLDSTEIN, S MITCHELL, P CROSSEN, JR WILLIAMS, PC TINDALL, A NEUWELT, EA TI MR AND COGNITIVE TESTING OF PATIENTS UNDERGOING OSMOTIC BLOOD-BRAIN-BARRIER DISRUPTION WITH INTRAARTERIAL CHEMOTHERAPY SO AMERICAN JOURNAL OF NEURORADIOLOGY LA English DT Article DE BLOOD-BRAIN BARRIER; CHEMOTHERAPY; IATROGENIC DISEASE OR DISORDER; BRAIN, MAGNETIC RESONANCE ID CELL LUNG-CANCER; PRIMARY CNS LYMPHOMA; WHITE MATTER LESIONS; CEREBRAL-ANGIOGRAPHY; RADIATION-THERAPY; SURVIVORS; TUMORS; ABNORMALITIES; CT; NEUROTOXICITY AB PURPOSE: To determine whether osmotic blood-brain barrier disruption is associated with MR abnormalities or cognitive deterioration and, if so, whether the MR findings correlate with cognitive test results, METHODS: Fifteen brain tumor patients who had a complete tumor response (nine central nervous system lymphoma, three germ cell and two astrocytoma, and one primitive neuroectodermal tumor) treated with blood-brain barrier disruption procedures (318 total procedures) with intraarterial chemotherapy were included. MR images were evaluated for the development of white matter hyperintensity, vascular lesions, or atrophy. Cognitive testing was performed to assess deterioration caused by this therapy, RESULTS: In two patients white matter hyperintensity developed, in two small vascular lesions developed, and in one mild atrophy developed. One infarct was asymptomatic and the second one resulted in mild dysesthesia in one upper extremity. No patient showed diminished cognitive function on the posttherapy evaluation. CONCLUSION: In patients undergoing blood-brain barrier disruption with intraarterial chemotherapy, new abnormalities on MR imaging may develop. These patients maintain the same level of cognitive and neurologic function and MR findings do not correlate with the results of cognitive testing. C1 OREGON HLTH SCI UNIV,DEPT NEUROL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT DIAGNOST RADIOL,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT MED PSYCHOL,PORTLAND,OR 97201. PORTLAND VA MED CTR,PORTLAND,OR. ROYAL MELBOURNE HOSP,DEPT RADIOL,PARKVILLE,VIC 3050,AUSTRALIA. ST PETERS COMMUNITY HOSP,DEPT NEUROSCI,HELENA,MT. FU PHS HHS [31770] NR 50 TC 28 Z9 28 U1 0 U2 2 PU AMER SOC NEURORADIOLOGY PI OAK BROOK PA 2210 MIDWEST RD, OAK BROOK, IL 60521 SN 0195-6108 J9 AM J NEURORADIOL JI Am. J. Neuroradiol. PD MAR PY 1995 VL 16 IS 3 BP 543 EP 553 PG 11 WC Clinical Neurology; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA QM240 UT WOS:A1995QM24000022 PM 7793380 ER PT J AU PEKARY, AE BERG, L WANG, JY LEE, P DUBINETT, SM HERSHMAN, JM AF PEKARY, AE BERG, L WANG, JY LEE, P DUBINETT, SM HERSHMAN, JM TI TNF-ALPHA, TSH, AND AGING REGULATE TGF-BETA SYNTHESIS AND SECRETION IN FRTL-5 RAT-THYROID CELLS SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY LA English DT Article DE CELL CULTURE; NORTHERN ANALYSIS; ENZYME-LINKED IMMUNOSORBENT ASSAY; BIOASSAY; APOPTOSIS ID TRANSFORMING GROWTH-FACTOR; TUMOR-NECROSIS-FACTOR; MESSENGER-RNA; EXPRESSION; DNA; FACTOR-BETA-1; APOPTOSIS; IODINE; LINE AB Tumor necrosis factor-alpha (TNF-alpha), a cytokine produced by macrophages in response to a variety of pathological conditions, can inhibit thyroid cell function in vitro and in vivo. TNF-alpha induction of transforming growth factor-beta 1 (TGF-beta 1) in rat endothelial cells suggested that TGF-beta, a known mediator of inflammatory effects of TNF-alpha may be involved in the sensitivity of aged thyroid cells to TNF-alpha (G. Chen, A. E. Pekary, and J. M. Hershman. Endocrinology 131: 863-870, 1992). To determine whether TNF-alpha induces TGF-beta production in FRTL-5 cells, young (< 20 passages) and aged (> 40 passages) FRTL-5 cells were grown to near confluency in medium containing 2 U/l of bovine thyroid-stimulating hormone [TSH; B-hormone (6H) medium] or no TSH [5-hormone (5H) medium]. TNF-alpha (0-100 ng/ml) was added 0-48 h before total RNA was extracted. Northern blots were hybridized with P-32-TGF-beta 1, -beta 2, and -beta 3 cDNAs. In aged cells TNF-alpha increased their TGF-beta 1 (and -beta 2 and -beta 3) mRNA levels 5.4-fold (and > 10-fold), respectively, while in young cells all TGF-beta mRNAs remained almost undetectable during incubation with TNF-alpha. In contrast, TNF-alpha and TSH had a highly significant stimulatory effect on the secretion rate of TGF-beta precursors in both young and old cells as measured in the mink lung cell bioassay. In summary, TNF-alpha and TSH enhance TGF-beta transcription and/or mRNA stability in aged, but not young, FRTL-5 cells, while TNF-alpha and TSH increase translation and secretion of TGF-beta in young and aged cells. C1 W LOS ANGELES VET AFFAIRS MED CTR, IMMUNOL PULM LAB, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90073 USA. RP PEKARY, AE (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, ENDOCRINE RES LAB, BLDG 114, RM 200, 11301 WILSHIRE BLVD, LOS ANGELES, CA 90073 USA. NR 46 TC 21 Z9 22 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6119 J9 AM J PHYSIOL-REG I JI Am. J. Physiol.-Regulat. Integr. Compar. Physiol. PD MAR PY 1995 VL 268 IS 3 BP R808 EP R815 PG 8 WC Physiology SC Physiology GA QL386 UT WOS:A1995QL38600029 PM 7900924 ER PT J AU CHRISTAKIS, NA ASCH, DA AF CHRISTAKIS, NA ASCH, DA TI PHYSICIAN CHARACTERISTICS ASSOCIATED WITH DECISIONS TO WITHDRAW LIFE-SUPPORT SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID SUSTAINING TREATMENT; CRITICALLY ILL; INTENSIVE-CARE; ATTITUDES; PREFERENCES; EUTHANASIA AB Objective. This study was undertaken to identify attributes of physicians associated with physicians' decisions to withdraw life support. Methods. Of the 862 Pennsylvania internists surveyed and asked to make decisions in response to hypothetical vignettes and to report their actual experience with the withdrawal of life support, 485 (56%) responded. The data were analyzed with regression models. Results With other factors controlled physicians were more willing to withdraw life support if they were young, practiced in a tertiary care setting or spent more time in clinical practice; they were less willing if they were Catholic or Jewish. Physicians reported a higher frequency of actually withdrawing life support if they were-young, had more contact with ICU patients, spent more time in clinical practice, or were specialists. Physicians with a greater willingness to withdraw were more likely to report having done so. Conclusions. Physicians' personal characteristics are associated with both their preferences and their practice in the withdrawal of life support, and a greater willingness to withdraw is associated with a higher frequency, of withdrawal. The influence of physician characteristics demonstrates that patient preferences and clinical circumstances do not exclusively-govern such ethical decisions. C1 UNIV PENN,SCH MED,DIV GEN INTERNAL MED,PHILADELPHIA,PA 19104. UNIV PENN,LEONARD DAVIS INST HLTH ECON,PHILADELPHIA,PA 19104. UNIV PENN,DEPT SOCIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP CHRISTAKIS, NA (reprint author), UNIV CHICAGO,MED CTR,ROOM W-700,5841 S MARYLAND AVE,CHICAGO,IL 60637, USA. RI Christakis, Nicholas/B-6690-2008; Christakis, Nicholas/C-3205-2009 NR 30 TC 112 Z9 113 U1 1 U2 2 PU AMER PUBLIC HEALTH ASSN INC PI WASHINGTON PA 1015 FIFTEENTH ST NW, WASHINGTON, DC 20005 SN 0090-0036 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAR PY 1995 VL 85 IS 3 BP 367 EP 372 DI 10.2105/AJPH.85.3.367 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA QM393 UT WOS:A1995QM39300017 PM 7892921 ER PT J AU HALL, PD LESHER, BA HALL, RK AF HALL, PD LESHER, BA HALL, RK TI ADJUVANT THERAPY OF NODE-NEGATIVE BREAST-CANCER SO ANNALS OF PHARMACOTHERAPY LA English DT Review ID COST-EFFECTIVENESS; TAMOXIFEN THERAPY; POSTMENOPAUSAL WOMEN; ESTROGEN-RECEPTORS; PROGNOSTIC FACTORS; DECISION-ANALYSIS; DOSE INTENSITY; BOWEL PROJECT; CATHEPSIN-D; FOLLOW-UP AB OBJECTIVE: To present the controversies regarding adjuvant cytotoxic chemotherapy or hormonal therapy in patients with node-negative breast cancer, and to evaluate the use of prognostic factors in identifying patients with node-negative breast cancer who will benefit from adjuvant therapy. DATA SOURCE: A MEDLINE search was performed to identify pertinent primary literature and review articles. Articles also were identified through Current Contents, textbooks, and bibliographies of selected articles. DATA EXTRACTION: The most recent clinical trials that evaluated cytotoxic chemotherapy or hormonal therapy in patients with node-negative breast cancer were chosen. Recent review articles and clinical trials that analyzed prognostic factors also were evaluated. DATA SYNTHESIS: The treatment of patients with node-negative breast cancer remains controversial. Approximately 60-80% of patients with this diagnosis will be alive 10 years after initial treatment without adjuvant therapy. The use of chemotherapy or hormonal therapy in node-negative disease increased after the 1988 National Cancer Institute Clinical Alert. Since that time, the research in node-negative breast cancer has focused on identifying prognostic factors and evaluating new treatment regimens. It is hoped that prognostic factors will help direct treatment decisions by identifying subgroups of patients who may benefit from adjuvant therapy. Prognostic factors currently being evaluated include tumor size, hormonal receptors, ploidy status, S-phase fraction, and cathepsin D. CONCLUSIONS: Many patients with node-negative breast cancer will be cured by local therapy alone. Even so, up to 58% of node-negative patients may develop recurrent disease. Reduction of breast cancer recurrence in patients with node-negative breast cancer has been documented as a result of adjuvant chemotherapy or tamoxifen. Of utmost priority is the identification of patients with node-negative breast cancer at highest risk for recurrence so that they may receive appropriate adjuvant therapy with curative intent, while sparing patients at lower risk for recurrence the toxic effects and financial burden incurred by unnecessary adjuvant treatment. C1 MED UNIV HOSP S CAROLINA,CHARLESTON,SC. HOLLINGS CANC CTR,CHARLESTON,IL. RALPH H JOHNSON VET AFFAIRS HOSP,CHARLESTON,IL. RP HALL, PD (reprint author), MED UNIV S CAROLINA,COLL PHARM,171 ASHLEY AVE,CHARLESTON,SC 29425, USA. NR 65 TC 2 Z9 2 U1 0 U2 0 PU HARVEY WHITNEY BOOKS CO PI CINCINNATI PA PO BOX 42696, CINCINNATI, OH 45242 SN 1060-0280 J9 ANN PHARMACOTHER JI Ann. Pharmacother. PD MAR PY 1995 VL 29 IS 3 BP 289 EP 298 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QN754 UT WOS:A1995QN75400011 PM 7606076 ER PT J AU BROTHERS, TE ROBISON, JG ELLIOTT, BM ARENS, C AF BROTHERS, TE ROBISON, JG ELLIOTT, BM ARENS, C TI IS INFRAPOPLITEAL BYPASS COMPROMISED BY DISTAL ORIGIN OF THE PROXIMAL ANASTOMOSIS SO ANNALS OF VASCULAR SURGERY LA English DT Article AB Distal origination of the proximal anastomosis (DOPA) of an infrapopliteal bypass beyond the adductor hiatus minimizes the length of graft required and optimizes use of scarce autogenous conduit. Sixty-two DOPA infrapopliteal revascularizations using autogenous vein performed for limb salvage over a 7-year period were reviewed and compared with 203 concurrent infrapopliteal bypasses originating more proximally (POPA). Life-table analysis revealed no difference at 54 months between DOPA and POPA bypass with regard to primary patency (57% vs. 50%, respectively) or secondary patency (67% vs. 65%, respectively). Differences in limb salvage at 54 months between DOPA and POPA bypasses did not reach statistical significance (53% vs. 66%, p = 0.12), although DOPA fared worse. Inferior limb salvage results in all infrapopliteal bypasses were correlated with the presence of tissue necrosis (52% vs. 70%, p < 0.001), which was more prevalent in patients undergoing DOPA bypass (71% vs. 49%, p < 0.01). The long-term patency of infrapopliteal bypass appears only marginally affected by DOPA. However, the prognosis for limb salvage in this setting is compromised as a result of the virulent behavior of the atherosclerotic disease that spares the superficial femoral artery while predominantly involving the popliteal and tibial vessels. C1 RALPH HENRY JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. MED UNIV S CAROLINA,CHARLESTON,SC 29425. RI Arens, Christoph/G-1796-2013 OI Arens, Christoph/0000-0001-8072-1438 NR 4 TC 11 Z9 11 U1 0 U2 0 PU QUALITY MEDICAL PUBLISHING INC PI ST LOUIS PA 11970 BORMAN DR, STE 222, ST LOUIS, MO 63146 SN 0890-5096 J9 ANN VASC SURG JI Ann. Vasc. Surg. PD MAR PY 1995 VL 9 IS 2 BP 172 EP 178 DI 10.1007/BF02139660 PG 7 WC Surgery; Peripheral Vascular Disease SC Surgery; Cardiovascular System & Cardiology GA QR281 UT WOS:A1995QR28100007 PM 7786703 ER PT J AU MENDEZ, MF DOSS, RC AF MENDEZ, MF DOSS, RC TI NEUROBEHAVIORAL ASPECTS OF THE DELAYED ENCEPHALOPATHY OF CARBON-MONOXIDE INTOXICATION - CASE-REPORT AND REVIEW SO BEHAVIOURAL NEUROLOGY LA English DT Note DE CARBON MONOXIDE; DEMYELINATION ID INTERVAL FORM; NEUROPSYCHIATRIC SEQUELAE; NEUROLOGIC SEQUELAE; PSYCHIC AKINESIA; FOLLOW-UP; BRAIN; TOMOGRAPHY; THERAPY; LESIONS; OXYGEN AB We report the neurobehavioral aspects of the delayed encephalopathy of carbon monoxide (CO) intoxication in a 29 year old woman and review the literature. Four weeks after CO poisoning, the patient developed a frontal lobe syndrome, visuoperceptual impairment, and diffuse white matter lesions with an otherwise normal neurological examination. In contrast, patients with the classical syndrome also have a parkinsonian state or an akinetic-mute state. The delayed encephalopathy of CO poisoning usually results from demyelination of subcortical white matter, necrosis of the globus pallidus, or both. The clinical aspects, risk factors, neurobiological features, and therapy and prognosis are discussed. C1 W LOS ANGELES VET AFFAIRS MED CTR,NEUROBEHAV UNIT 691116AF,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. ST PAUL RAMSEY MED CTR,ST PAUL,MN 55101. NR 45 TC 0 Z9 0 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0953-4180 J9 BEHAV NEUROL JI Behav. Neurol. PD SPR PY 1995 VL 8 IS 1 BP 47 EP 52 PG 6 WC Clinical Neurology SC Neurosciences & Neurology GA QP683 UT WOS:A1995QP68300007 PM 24487403 ER PT J AU BARCHIESI, F HOLLIS, RJ MCGOUGH, DA SCALISE, G RINALDI, MG PFALLER, MA AF BARCHIESI, F HOLLIS, RJ MCGOUGH, DA SCALISE, G RINALDI, MG PFALLER, MA TI DNA SUBTYPES AND FLUCONAZOLE SUSCEPTIBILITIES OF CANDIDA-ALBICANS ISOLATES FROM THE ORAL CAVITIES OF PATIENTS WITH AIDS SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID ACQUIRED-IMMUNODEFICIENCY-SYNDROME; RESISTANT CANDIDA; THERAPY; VIRUS; INDIVIDUALS; BIOTYPES AB Sixty-two Candida albicans isolates from the oral cavities of 28 patients with AIDS who were receiving fluconazole therapy were typed by restriction endonuclease analysis followed by pulsed-field gel electrophoresis; these isolates were then tested for fluconazole susceptibility by a standard broth dilution method. Sequential isolates (range, 2-4) were evaluated for 22 patients; only one isolate was evaluated for six patients. DNA subtyping revealed a total of 37 different DNA subtypes. Twelve (54.5%) of 22 patients with multiple episodes of oropharyngeal candidiasis were infected with a single DNA subtype throughout the observation period. Ten (45.5%) of 22 patients with multiple episodes of oropharyngeal candidiasis were infected with two or three DNA subtypes during the observation period. In vitro susceptibility tests revealed that MICs of fluconazole ranged from less than or equal to 0.125 mu g/mL to 64 mu g/mL, with an MIC(50) of 0.5 mu g/mL and an MIC(90) of 4 mu g/mL. A significant increase in the MICs (fourfold or greater) of fluconazole for sequential C. albicans isolates was found for 66.6% of the patients infected with a single DNA subtype and for 50% of the patients infected with multiple DNA subtypes. Despite a limited number of patients and isolates, our data suggest that C. albicans isolates that are susceptible to fluconazole at MICs of greater than or equal to 8 mu g/mL in vitro will be less susceptible in vivo to standard doses (100-200 mg/d) of this drug. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV IOWA,COLL MED,DEPT PATHOL,SPECIAL MICROBIOL LAB,IOWA CITY,IA. UNIV ANCONA,IST MALATTIE INFETT & MED PUBBL,ANCONA,ITALY. RP BARCHIESI, F (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284, USA. NR 23 TC 57 Z9 57 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD MAR PY 1995 VL 20 IS 3 BP 634 EP 640 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA QL159 UT WOS:A1995QL15900026 PM 7756488 ER PT J AU HOWARITZ, JH AF HOWARITZ, JH TI SERUM TUMOR-MARKERS - IMPLICATIONS OF DISCORDANT RESULTS SO DISEASE MARKERS LA English DT Editorial Material ID PROSTATE-SPECIFIC ANTIGEN; CARCINOEMBRYONIC ANTIGEN; STANDARDIZATION; IMMUNOASSAYS; GONADOTROPIN; ASSAYS RP HOWARITZ, JH (reprint author), UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,PATHOL & LAB MED SERV 113,LOS ANGELES,CA, USA. NR 12 TC 0 Z9 0 U1 0 U2 0 PU ASFRA PI EDAM PA VOORHAVEN 33, 1135 BL EDAM, NETHERLANDS SN 0278-0240 J9 DIS MARKERS JI Dis. Markers PD MAR PY 1995 VL 12 IS 2 BP 81 EP 83 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental; Pathology SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine; Pathology GA QV431 UT WOS:A1995QV43100001 PM 7614784 ER PT J AU LEHNER, LA BURGESS, JF AF LEHNER, LA BURGESS, JF TI TEACHING AND HOSPITAL PRODUCTION - THE USE OF REGRESSION ESTIMATES SO HEALTH ECONOMICS LA English DT Article; Proceedings Paper CT 1st Annual Health-Economics-Committee Northeast Regional Research Symposium CY SEP 03, 1992 CL NEWPORT, RI SP US DEPT VET AFFAIRS, HLTH ECON COMM DE PRODUCTION; REGRESSION ANALYSIS; LABOR REQUIREMENTS; PROSPECTIVE PAYMENT SYSTEM; TEACHING HOSPITALS; MEDICAL EDUCATION ID PROSPECTIVE PAYMENT; COSTS; SPECIFICATION; VARIABLES; ERRORS; SYSTEM AB Medicare's Prospective Payment System pays U.S. teaching hospitals for the indirect costs of medical education based on a regression coefficient in a cost function. In regression studies using health care data, it is common for explanatory variables to be measured imperfectly, yet the potential for measurement error is often ignored. In this paper, U.S. Department of Veterans Affairs data is used to examine issues of health care production estimation and the use of regression estimates like the teaching adjustment factor. The findings show that measurement error and persistent multicollinearity confound attempts to have a large degree of confidence in the precise magnitude of parameter estimates. RP LEHNER, LA (reprint author), US DEPT VET AFFAIRS,MANAGEMENT SCI GRP,200 SPRINGS RD,BEDFORD,MA 01730, USA. NR 25 TC 9 Z9 9 U1 1 U2 1 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 1057-9230 J9 HEALTH ECON JI Health Econ. PD MAR-APR PY 1995 VL 4 IS 2 BP 113 EP 125 DI 10.1002/hec.4730040204 PG 13 WC Economics; Health Care Sciences & Services; Health Policy & Services SC Business & Economics; Health Care Sciences & Services GA QV095 UT WOS:A1995QV09500004 PM 7613596 ER PT J AU MOZDZIERZ, GJ AF MOZDZIERZ, GJ TI GREETINGS SO INDIVIDUAL PSYCHOLOGY-THE JOURNAL OF ADLERIAN THEORY RESEARCH & PRACTICE LA English DT Editorial Material C1 LOYOLA UNIV,STRITCH SCH MED,MAYWOOD,IL 60153. RP MOZDZIERZ, GJ (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,PSYCHOL SERV 116B,ROOSEVELT RD & 5TH AVE,HINES,IL 60141, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU UNIV TEXAS PRESS PI AUSTIN PA BOX 7819, AUSTIN, TX 78713-7819 SN 0277-7010 J9 INDIV PSYCHOL JI Individ. Psychol.- J. Adlerian Theory Res. Pract. PD MAR PY 1995 VL 51 IS 1 BP 1 EP 3 PG 3 WC Psychology SC Psychology GA RQ189 UT WOS:A1995RQ18900001 ER PT J AU LAWRENCE, O RACHIE, N QURESHI, N BOMSZTYK, K SIBLEY, CH AF LAWRENCE, O RACHIE, N QURESHI, N BOMSZTYK, K SIBLEY, CH TI DIPHOSPHORYL LIPID-A FROM RHODOBACTER-SPHAEROIDES TRANSIENTLY ACTIVATES NF-KAPPA-B BUT INHIBITS LIPOPOLYSACCHARIDE INDUCTION OF KAPPA-LIGHT-CHAIN AND OCT-2 IN THE B-CELL LYMPHOMA LINE-70Z/3 SO INFECTION AND IMMUNITY LA English DT Article ID SURFACE-IMMUNOGLOBULIN EXPRESSION; DNA-BINDING SUBUNIT; RHODOPSEUDOMONAS-SPHAEROIDES; GENE-TRANSCRIPTION; NONTOXIC LIPOPOLYSACCHARIDE; CONSERVED SEQUENCE; FATTY-ACIDS; PROTEIN; ENHANCER; CD14 AB Lipopolysaccharide (LPS) is implicated in much of the pathophysiology associated with gram-negative septic shock One approach to this serious clinical problem is to develop new drugs that antagonize the action of toxic LPS. A model system to study LPS action and test for potential antagonists is readily provided by LPS regulation of the kappa gene in the murine B-cell line 70Z/3. Rhodobacter sphaeroides diphosphoryl lipid A (RsDPLA) effectively blocked toxic LPS induction of kappa light-chain immunoglobulin expression in 70Z/3 cells. Induction of kappa expression by LPS is dependent on the activation of at least two transcription factors, Oct-2 and NF-KB. RsDPLA completely repressed the long-term activation of NF-KB observed after 24 h of Salmonella typhosa LPS treatment and antagonized activation of oct-2 mRNA expression. However, RsDPLA was not an inert competitor of LPS. RsDPLA alone strongly activated NP KB binding activity by 30 min but not beyond 9 h of treatment. It also induced a small increase in oct-a mRNA levels. RsDPLA is not simply a weak agonist; we found no graded increase in kappa expression with increasing RsDPLA concentrations up to 50 mu g/ml. The NF-KB complexes activated by RsDPLA and S. typhosa LPS were both composed of the p50-p65 heterodimer. These results suggest that the physiological LPS receptor(s) on B cells transmits qualitatively different signals depending on the nature of the binding ligand and that the fatty acyl groups of LPS play an important role in activating signal transduction. C1 UNIV WASHINGTON,DEPT GENET,SEATTLE,WA 98195. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL RES LAB,MADISON,WI 53705. UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI 53706. FU NIAID NIH HHS [AI08626]; NIGMS NIH HHS [GM50870, GM45134] NR 50 TC 12 Z9 12 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD MAR PY 1995 VL 63 IS 3 BP 1040 EP 1046 PG 7 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA QJ524 UT WOS:A1995QJ52400043 PM 7868225 ER PT J AU WENZEL, UO FOUQUERAY, B BISWAS, P GRANDALIANO, G CHOUDHURY, GG ABBOUD, HE AF WENZEL, UO FOUQUERAY, B BISWAS, P GRANDALIANO, G CHOUDHURY, GG ABBOUD, HE TI ACTIVATION OF MESANGIAL CELLS BY THE PHOSPHATASE INHIBITOR VANADATE - POTENTIAL IMPLICATIONS FOR DIABETIC NEPHROPATHY SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE PLATELET-DERIVED GROWTH FACTOR; HUMAN KIDNEY; TYROSINE PHOSPHORYLATION; PROTEIN KINASE C; PHOSPHOLIPASE C ID PROTEIN TYROSINE PHOSPHATASES; FIBROBLAST GROWTH-FACTOR; DNA-SYNTHESIS; MESSENGER-RNAS; RAT ADIPOCYTES; PDGF RECEPTOR; FACTOR-BETA; KINASE-C; EXPRESSION; KIDNEY AB The metalion vanadate has insulin-like effects and has been advocated for use in humans as a therapeutic modality for diabetes mellitus. However, since vanadate is a tyrosine phosphatase inhibitor, it may result in undesirable activation of target cells. We studied the effect of vanadate on human mesangial cells, an important target in diabetic nephropathy. Vanadate stimulated DNA synthesis and PDGF B chain gene expression. Vanadate also inhibited total tyrosine phosphatase activity and stimulated tyrosine phosphorylation of a set of cellular proteins. Two chemically and mechanistically dissimilar tyrosine kinase inhibitors, genistein and herbimycin A, blocked DNA synthesis induced by vanadate. Vanadate also stimulated phospholipase C and protein kinase C. Downregulation of protein kinase C abolished vanadate-induced DNA synthesis. Thus, vanadate-induced mitogenesis is dependent on tyrosine kinases and protein kinase C activation. The most likely mechanism for the effect of vanadate on these diverse processes involves the inhibition of cellular phosphotyrosine phosphatases. These studies demonstrating that vanadate activates mesangial cells may have major implications for the therapeutic potential of vanadate administration in diabetes. Although vanadate exerts beneficial insulin-like effects and potentiates the effect of insulin in sensitive tissue, it may result in undesirable activation of other target cells, such as mesangial cells. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV NEPHROL,SAN ANTONIO,TX 78284. AUDIE L MURPHY VET AFFAIRS HOSP,SAN ANTONIO,TX. UNIV HOSP TEXAS,DEPT MED,DIV NEPHROL,SAN ANTONIO,TX 78284. RI Grandaliano, Giuseppe/G-2963-2012 FU NIDDK NIH HHS [DK-33665, DK-43988] NR 50 TC 31 Z9 32 U1 0 U2 1 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD MAR PY 1995 VL 95 IS 3 BP 1244 EP 1252 DI 10.1172/JCI117774 PG 9 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA QM012 UT WOS:A1995QM01200044 PM 7883973 ER PT J AU COLOMBO, AL BARCHIESI, F MCGOUGH, DA RINALDI, MG AF COLOMBO, AL BARCHIESI, F MCGOUGH, DA RINALDI, MG TI COMPARISON OF E-TEST AND NATIONAL-COMMITTEE-FOR-CLINICAL-LABORATORY-STANDARDS BROTH MACRODILUTION METHOD FOR AZOLE ANTIFUNGAL SUSCEPTIBILITY TESTING SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID FUNGAL-INFECTIONS; MULTICENTER EVALUATION; AGAR DILUTION; FLUCONAZOLE; TRANSPLANTATION; MICRODILUTION; YEASTS AB The use of Etest strips for antimicrobial susceptibility testing is a new and promising method with broad applications in microbiology. Since these strips contain a predefined continuous gradient of a drug, it is possible to obtain a reproducible, quantitative MIC reading. We performed a prospective and double-blinded study to compare the Etest and National Committee for Clinical Laboratory Standards (Villanova, Pa.) broth macrodilution methods for determining the MICs of fluconazole, itraconazole, and ketoconazole for 100 clinical isolates (25 Candida albicans, 25 Cryptococcus neofarmans var, neoformans, 20 Torulopsis [Candida] glabrata, 15 Candida tropicalis, and 15 Candida parapsilosis). The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinical Laboratory Standards document M27-P guidelines. Despite differences between results for some species-drug combinations, Etest and macrobroth MICs were, in general, in good agreement. The MIC agreement rates for the two methods, within +/-1 dilution, were 71% for ketoconazole, 80% for fluconazole, and 84% for itraconazole. According to our data, Etest has potential utility as an alternative method. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. UNIV ANCONA,IST MALATTIE INFETT & MED PUBBL,ANCONA,ITALY. RP COLOMBO, AL (reprint author), ESCOLA PAULISTA MED,DISCIPLINA DOENCAS INFECCIOSAS & PARASITARIAS,RUA BOTUCATU 740,BR-04023062 SAO PAULO,BRAZIL. NR 25 TC 106 Z9 116 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1995 VL 33 IS 3 BP 535 EP 540 PG 6 WC Microbiology SC Microbiology GA QG830 UT WOS:A1995QG83000005 PM 7751353 ER PT J AU KRISHER, KK HOLDRIDGE, NB MUSTAFA, MM RINALDI, MG MCGOUGH, DA AF KRISHER, KK HOLDRIDGE, NB MUSTAFA, MM RINALDI, MG MCGOUGH, DA TI DISSEMINATED MICROASCUS-CIRROSUS INFECTION IN PEDIATRIC BONE-MARROW TRANSPLANT RECIPIENT SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Note ID IMMUNOCOMPROMISED HOST; SCOPULARIOPSIS AB Microascus cirrosus Curzi and its associated anamorphic state, Scopulariopsis, were recovered from the cutaneous lesion of a 12-year-old male who had undergone an autologous bone marrow transplantation for acute myelogenous leukemia. Histopathology sections from the biopsied lesion demonstrated septate hyphae consistent with a fungal etiology. Radiographic studies of the lungs subsequent to progression of the lesion revealed a consolidation in the right upper lobe suggesting a primary focus of infection. While M. cirrosus is fairly abundant in nature and widely distributed in stored grains in North America, this is, to our knowledge, the first reported human infection by this species. Salient characteristics for the identification of this dematiaceous ascomycete, M. cirrosus, will be presented. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,FUNGUS TESTING LAB,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP KRISHER, KK (reprint author), CHILDRENS MED CTR,1935 MOTOR ST,DALLAS,TX 75235, USA. NR 12 TC 25 Z9 25 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAR PY 1995 VL 33 IS 3 BP 735 EP 737 PG 3 WC Microbiology SC Microbiology GA QG830 UT WOS:A1995QG83000043 PM 7751387 ER PT J AU CACCIOLA, JS ALTERMAN, AI RUTHERFORD, MJ SNIDER, EC AF CACCIOLA, JS ALTERMAN, AI RUTHERFORD, MJ SNIDER, EC TI TREATMENT RESPONSE OF ANTISOCIAL SUBSTANCE-ABUSERS SO JOURNAL OF NERVOUS AND MENTAL DISEASE LA English DT Article ID DIAGNOSTIC INTERVIEW SCHEDULE; SEEKING COCAINE ABUSERS; PERSONALITY-DISORDER; ALCOHOLICS; PSYCHOPATHOLOGY; FOLLOW; SEVERITY; VALIDITY; ADDICTS AB We evaluated the relationship of antisociality to 7-month treatment response in 224 alcohol- and/or cocaine-dependent men. Subjects with and without a DSM-III antisocial personality disorder (ASPD) responded similarly and positively to treatment in a number of functional domains, including substance use. A more detailed analysis comparing subjects with ASPD, subjects meeting adult but not childhood ASPD criteria (A-ASPD), and subjects meeting neither adult nor childhood ASPD criteria (pure non-ASPD) revealed similar and positive responses to treatment among the three groups. The antisocial groups had more cocaine and alcohol use at the baseline evaluation, but at 7-month follow-up, they had levels of use not significantly different than the pure non-ASPD group. The findings suggest that an ASPD diagnosis or an adult antisocial lifestyle, at least as measured by DSM-III criteria, does not predict short-term treatment response. C1 UNIV PENN,SCH MED,DEPT PSYCHIAT,PENN VET AFFAIRS CTR STUDIES ADDICT,PHILADELPHIA,PA. VET AFFAIRS MED CTR,PENN VET AFFAIRS CTR STUDIES ADDICT,PHILADELPHIA,PA. NR 29 TC 67 Z9 67 U1 1 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3018 J9 J NERV MENT DIS JI J. Nerv. Ment. Dis. PD MAR PY 1995 VL 183 IS 3 BP 166 EP 171 DI 10.1097/00005053-199503000-00007 PG 6 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QM559 UT WOS:A1995QM55900007 PM 7891063 ER PT J AU CHEYETTE, SR CUMMINGS, JL AF CHEYETTE, SR CUMMINGS, JL TI ENCEPHALITIS LETHARGICA - LESSONS FOR CONTEMPORARY NEUROPSYCHIATRY SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID PARKINSONS-DISEASE; DEPRESSION; DOPAMINE; DISORDER AB Encephalitis lethargica (von Economo's encephalitis), pandemic from 1917 to 1926, opened a window on the study of behavioral consequences of infection-induced subcortical disorder. Widely varying acute manifestations included extra-pyramidal disorders, myoclonus, eye movement disorders, paralyses, delirium, mood changes, inverted diurnal rhythms, and catatonia. Major pathological changes involved the substantia nigra, globus pallidus, and hypothalamus. A symptom-free recovery period was often followed by postencephalitic disturbances, typically parkinsonism in adults and conduct disorder in children. Occurrence of depression, mania, obsessive-compulsive disorder, and hyperactivity in post-encephalitic patients anticipated current concepts of the role of the basal ganglia in mood, personality, and obsessional syndromes. Observations of deferred onset and ''tardy'' hyperkinesias presaged current theories of the pathophysiology of tardive dyskinesia. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,BEHAV NEUROSCI SECT,LOS ANGELES,CA. RP CHEYETTE, SR (reprint author), UNIV CALIF LOS ANGELES,SCH MED,REED NEUROL RES CTR,DEPT NEUROL,710 WESTWOOD PLAZA,LOS ANGELES,CA 90024, USA. NR 82 TC 46 Z9 46 U1 3 U2 7 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD SPR PY 1995 VL 7 IS 2 BP 125 EP 134 PG 10 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QV776 UT WOS:A1995QV77600001 PM 7626955 ER PT J AU HINKIN, CH VANGORP, WG MANDELKERN, MA GEE, M SATZ, P HOLSTON, S MARCOTTE, TD EVANS, G PAZ, DH ROPCHAN, JR QUINONES, N KHONSARY, A BLAHD, WH AF HINKIN, CH VANGORP, WG MANDELKERN, MA GEE, M SATZ, P HOLSTON, S MARCOTTE, TD EVANS, G PAZ, DH ROPCHAN, JR QUINONES, N KHONSARY, A BLAHD, WH TI CEREBRAL METABOLIC CHANGE IN PATIENTS WITH AIDS - REPORT OF A 6-MONTH FOLLOW-UP USING POSITRON-EMISSION-TOMOGRAPHY SO JOURNAL OF NEUROPSYCHIATRY AND CLINICAL NEUROSCIENCES LA English DT Article ID IMMUNODEFICIENCY-VIRUS INFECTION; BLOOD-BRAIN-BARRIER; DEMENTIA COMPLEX; NEUROPSYCHOLOGICAL FINDINGS; ASYMPTOMATIC SUBJECTS; HIV-1 INFECTION; HOMOSEXUAL MEN; ALZHEIMER TYPE; HTLV-III; PERFORMANCE AB The authors examined change in cerebral metabolic function over time by using PET in HIV-infected individuals diagnosed with AIDS. Ten subjects with AIDS received [F-18]2-fluoro-2-deoxy-D-glucose PET scans and completed a comprehensive neuropsychological battery. The scan and test battery were repeated after 6 months. Over time, the subjects showed increased relative basal ganglia metabolism as well as increased parietal lobe metabolism. There were no statistically significant changes in neuropsychological performance. These results suggest that PET may be move sensitive than traditional neuropsychological evaluation to subtle central nervous system changes in AIDS. C1 W LOS ANGELES DEPT VET AFFAIRS MED CTR,LOS ANGELES,CA. UNIV CALIF IRVINE,DEPT PHYS,IRVINE,CA 92717. RP HINKIN, CH (reprint author), UNIV CALIF LOS ANGELES,SCH MED,NPI,ROOM C8-747,760 WESTWOOD PLAZA,LOS ANGELES,CA 90024, USA. NR 47 TC 49 Z9 50 U1 2 U2 2 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0895-0172 J9 J NEUROPSYCH CLIN N JI J. Neuropsychiatr. Clin. Neurosci. PD SPR PY 1995 VL 7 IS 2 BP 180 EP 187 PG 8 WC Clinical Neurology; Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA QV776 UT WOS:A1995QV77600007 PM 7626961 ER PT J AU HEYDARI, AR CONRAD, CC RICHARDSON, A AF HEYDARI, AR CONRAD, CC RICHARDSON, A TI EXPRESSION OF HEAT-SHOCK GENES IN HEPATOCYTES IS AFFECTED BY AGE AND FOOD RESTRICTION IN RATS SO JOURNAL OF NUTRITION LA English DT Article DE LIVER; FOOD RESTRICTION; RATS; HEAT SHOCK PROTEINS; UBIQUITIN ID GLUCOSE-REGULATED PROTEIN; UBIQUITIN MESSENGER-RNA; DIETARY RESTRICTION; TRANSCRIPTIONAL REGULATION; HSP70-LIKE PROTEIN; CELLS; STRESS; FAMILY; LIVER; FIBROBLASTS AB The objective of this study was to determine how food restriction altered the age-related changes in the basal and heat-induced expression of heat shock genes (hsc70, grp78, and ubiquitin) by hepatocytes isolated from young (4 to 6 mo old) and old (26 to 28 mo old) male Fischer 344 rats. The basal levels of the mRNA transcripts for hsc70 and ubiquitin were similar for hepatocytes isolated from young and old rats fed with free access to the diet (control) or from rats fed a restricted diet (40% restriction of food intake). However, the induction of the mRNA transcripts for hsc70 and ubiquitin by a heat shock (42.5 degrees C for 30 min) was significantly higher for hepatocytes isolated from old rats fed the restricted diet compared with old rats fed with free access to the diet. The changes in hsc70 mRNA levels were paralleled by similar changes in hsc70 transcription by isolated nuclei; therefore, the greater induction of hsc70 expression by food restriction results from changes in the transcription of hsc70 gene. In contrast with hsc70 and ubiquitin, the basal levels of grp78 were reduced with age and by food restriction. Therefore, the effect of aging and food restriction on the basal and heat-induced expression of heat shock genes varies considerably from gene to gene. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. RP HEYDARI, AR (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284, USA. FU NIA NIH HHS [AG01548]; NIEHS NIH HHS [ES06277] NR 35 TC 42 Z9 44 U1 0 U2 3 PU AMER INST NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAR PY 1995 VL 125 IS 3 BP 410 EP 418 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA QL021 UT WOS:A1995QL02100002 PM 7876915 ER PT J AU DICKERSON, RN MANZO, CB CHARLAND, SL SETTLE, RG STEIN, TP KUHL, DA RAJTER, JJ AF DICKERSON, RN MANZO, CB CHARLAND, SL SETTLE, RG STEIN, TP KUHL, DA RAJTER, JJ TI THE EFFECT OF INSULIN-LIKE GROWTH-FACTOR-I ON PROTEIN-METABOLISM AND HEPATIC RESPONSE TO ENDOTOXEMIA IN PARENTERALLY FED RATS SO JOURNAL OF SURGICAL RESEARCH LA English DT Article ID ACUTE PHASE RESPONSE; ENERGY-METABOLISM; SKELETAL-MUSCLE; SEPTIC RATS; FACTOR-I; 3-METHYLHISTIDINE; NUTRITION; NITROGEN; SEPSIS; EXPRESSION AB To determine the influence of insulin-like growth factor-1 (IGF-1) on nitrogen loss and hepatic response to critical illness, 34 male Sprague-Dawley rats (190-230 g) were randomized to receive parenteral nutrition (PN) only (Ctrl), PN plus continuous infusion of Escherichia coli 026:B6 lipopolysaccharide at 6 mg/kg/day (LPS), or PN plus LPS plus rhIGF-1 (IGF-1) at 3 mg/kg/day for 48 hr. Prior to randomization, all animals underwent iv cannulation and 30 hr of adaptation to PN. All animals received isocaloric and isonitrogenous PN (glucose 170 kcal/kg/day and nitrogen 1.1 g/kg/day) and were kept NPO except for water ad libitum, [N-15]glycine was infused in all animals for determination of liver fractional synthetic rate. Cumulative nitrogen balance during endotoxemia was significantly different from each other (+72 +/- 42, -217 +/- 131, -114 +/- 137 mg/kg/48 hr for the Ctrl, LPS, and IGF-1 groups, respectively; ANOVA, P < 0.001). Endotoxin significantly increased the urinary 3-methylhistidine/creatinine ratio (0.24 +/- 0.05, 0.55 +/- 0.12, 0.48 +/- 0.17 for the Ctrl, LPS, and IGF-1 groups, respectively; ANOVA, P < 0.001); however, IGF-1 did not significantly reduce the ratio. Endotoxin induced a significant increase in liver fractional synthetic rate (29 +/- 8, 56 +/- 18, 64 +/- 12%/day for the Ctrl, LPS, and IGF-1 groups, respectively; ANOVA, P < 0.01) and depressed hepatic cytochrome P450 concentration (0.54 +/- 0.19, 0.22 +/- 0.07, 0.19 +/- 0.07 nmol/mg protein, respectively; ANOVA, P < 0.05) and ethoxycoumarin O-deethylase (ECOD) activity (103 +/- 73, 29 +/- 13, 17 +/- 11, pmol/mg/min, respectively; ANOVA, P < 0.01); however, rhIGF-1 did not significantly alter these hepatic variables during endotoxin infusion. Recombinant human insulin-like growth factor-1 significantly improved nitrogen balance without compromising hepatic response as measured by liver fractional synthetic rate, cytochrome P450 concentration, and ECOD activity in endotoxemic parenterally fed rats. (C) 1995 Academic Press, Inc. C1 PHILADELPHIA VET AFFAIRS MED CTR,PHILADELPHIA COLL PHARM & SCI,PHILADELPHIA,PA. UNIV MED & DENT NEW JERSEY,CAMDEN,NJ. RP DICKERSON, RN (reprint author), UNIV TENNESSEE,DEPT CLIN PHARM,26 S DUNLAP ST,MEMPHIS,TN 38163, USA. RI Dickerson, Roland/C-5185-2008 FU NIDDK NIH HHS [1R15DK46545-01] NR 37 TC 20 Z9 20 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0022-4804 J9 J SURG RES JI J. Surg. Res. PD MAR PY 1995 VL 58 IS 3 BP 260 EP 266 DI 10.1006/jsre.1995.1041 PG 7 WC Surgery SC Surgery GA QL031 UT WOS:A1995QL03100002 PM 7885022 ER PT J AU CHAVIN, SI AF CHAVIN, SI TI ERYTHROPOIETIN THERAPY FOR ANEMIA SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Letter C1 DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA. RP CHAVIN, SI (reprint author), MED COLL PENN,PHILADELPHIA,PA, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD MAR PY 1995 VL 43 IS 3 BP 314 EP 315 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QK439 UT WOS:A1995QK43900025 PM 7884127 ER PT J AU TAN, AU COHEN, AH LEVINE, BS AF TAN, AU COHEN, AH LEVINE, BS TI RENAL AMYLOIDOSIS IN A DRUG-ABUSER SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Article DE AMYLOIDOSIS; DRUG ABUSE; NEPHROTIC SYNDROME; COLCHICINE ID FAMILIAL MEDITERRANEAN FEVER; PRIMARY SYSTEMIC AMYLOIDOSIS; COLCHICINE THERAPY; TRANSPLANTATION; DIMETHYLSULFOXIDE; EXPERIENCE; SURVIVAL AB Drug abusers, particularly those who inject drugs s.c. (''skin popping''), may develop amyloidosis, Chronic infections are thought to play a pathogenetic role in this setting. A patient is presented who had a history of ''skin popping'' cocaine and heroin and developed nephrotic syndrome, with an elevated serum creatinine and a creatinine clearance of 61 ml/min. Renal biopsy demonstrated amyloidosis. Treatment with colchicine was initiated, and proteinuria decreased to near normal levels after 12 months, Concomitant with the decrease in proteinuria, creatinine clearance improved, although a repeat renal biopsy failed to show any significant improvement in amyloid burden. These observations suggest that colchicine may be a useful treatment in reversing the proteinuria of renal amyloidosis associated with drug abuse. Furthermore, clinical improvement may occur before any demonstrable regression in the amyloidosis. C1 W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. CEDARS SINAI MED CTR,DEPT MED,LOS ANGELES,CA 90048. CEDARS SINAI MED CTR,DEPT PATHOL,LOS ANGELES,CA 90048. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. NR 25 TC 19 Z9 20 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD MAR PY 1995 VL 5 IS 9 BP 1653 EP 1658 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA QM947 UT WOS:A1995QM94700003 PM 7780053 ER PT J AU APPLEBY, J AF APPLEBY, J TI MANAGEMENT OF THE ABNORMAL PAPANICOLAOU SMEAR SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Article ID IMMUNODEFICIENCY-VIRUS-INFECTION; CERVICAL INTRAEPITHELIAL NEOPLASIA; PAP SMEAR; BACTERIAL VAGINOSIS; SEROPOSITIVE WOMEN; CANCER DETECTION; CERVICOGRAPHY; RISK; COLPOSCOPY; PREVENTION AB This article briefly reviews the natural history and epidemiology of cervical neoplasia and discusses the strengths and weaknesses of cytologic screening. The authors also review systems of reporting cytology results and outline approaches to management of the abnormal smear, including management of infectious, inflammatory, atypical, and remalignant changes. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP APPLEBY, J (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV GEN MED,SAN ANTONIO,TX 78284, USA. NR 68 TC 5 Z9 5 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAR PY 1995 VL 79 IS 2 BP 345 EP 360 PG 16 WC Medicine, General & Internal SC General & Internal Medicine GA QN027 UT WOS:A1995QN02700009 PM 7877395 ER PT J AU OKEEFE, M HUNT, DK AF OKEEFE, M HUNT, DK TI ASSESSMENT AND TREATMENT OF IMPOTENCE SO MEDICAL CLINICS OF NORTH AMERICA LA English DT Review ID NOCTURNAL PENILE TUMESCENCE; VACUUM CONSTRICTION DEVICE; NEGATIVE-PRESSURE DEVICE; ERECTILE IMPOTENCE; SEXUAL DYSFUNCTION; BRACHIAL INDEX; BULBOCAVERNOSUS REFLEX; VASCULOGENIC IMPOTENCE; DUPLEX ULTRASONOGRAPHY; ARTERIOGENIC IMPOTENCE AB Impotence is a common problem but is often neglected or inappropriately assessed and treated. Assessment should be focused toward causes of impotence that have specific treatments: psychogenic, drug induced, hormonal, and vascular. Specific treatments available are sexual counseling, medication adjustment, hormone therapy, and vascular surgery. When impotence is neuropathic, of mixed etiology and/or unresponsive to disease-specific treatments, therapy should be determined by patient preference. Options include vacuum erection devices, penile self-injection therapy, and penile prosthesis. There is less evidence supporting the use of yohimbine, topical nitrates, or minoxidil. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. RP OKEEFE, M (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,OFF AMBULATORY CARE 11C,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 104 TC 18 Z9 18 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0025-7125 J9 MED CLIN N AM JI Med. Clin. N. Am. PD MAR PY 1995 VL 79 IS 2 BP 415 EP 434 PG 20 WC Medicine, General & Internal SC General & Internal Medicine GA QN027 UT WOS:A1995QN02700013 PM 7877399 ER PT J AU GREEN, RC BENJAMIN, S CUMMINGS, JL AF GREEN, RC BENJAMIN, S CUMMINGS, JL TI FELLOWSHIP PROGRAMS IN BEHAVIORAL NEUROLOGY SO NEUROLOGY LA English DT Review ID NEUROPSYCHIATRY AB We sent a behavioral neurology fellowship questionnaire to each of the training directors of 160 neurology residency programs throughout the world, seeking information about programs offering advanced training in behavioral neurology (or similar fellowships in cognitive neurology, neurobehavior, or cognitive neuroscience). Response rate was 100%. Thirty-four respondents reported active fellowship programs in behavioral neurology, and 28 additional respondents indicated that a behavioral neurology fellowship was planned. Nine of the 34 programs (26.5%) defined themselves as exclusively or predominantly concerned with dementia and age-related neurobehavioral disorders. Directors of the 34 active fellowship programs estimated that their combined programs had graduated 199 fellows and were currently training fifty. Most fellowships concentrated on outpatient clinical training, with teaching required by 78.1% and research required by 81.8%. Specialty certification for behavioral neurology was favored by over 75% of behavioral neurology fellowship training directors but by only 30% of training directors in residency programs without behavioral neurology fellowships. Behavioral neurology training programs have grown dramatically in response to an increased recognition of the academic interest in and the clinical needs for these services. C1 EMORY UNIV,SCH MED,DEPT NEUROL,NEUROBEHAV PROGRAM,ATLANTA,GA 30322. UNIV MASSACHUSETTS,MED CTR,DEPT PSYCHIAT,NEUROPSYCHIAT PROGRAM,WORCESTER,MA 01655. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV,BEHAV NEUROSCI SECT,LOS ANGELES,CA 90073. RP GREEN, RC (reprint author), EMORY UNIV,WESLEY WOODS CTR,NEUROBEHAV PROGRAM,1841 CLIFTON RD NE,ATLANTA,GA 30329, USA. FU NIA NIH HHS [P30AG10130] NR 10 TC 2 Z9 2 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAR PY 1995 VL 45 IS 3 BP 412 EP 415 PN 1 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA QM902 UT WOS:A1995QM90200002 PM 7898686 ER PT J AU KINOSIAN, B JEEJEEBHOY, KN AF KINOSIAN, B JEEJEEBHOY, KN TI WHAT IS MALNUTRITION - DOES IT MATTER SO NUTRITION LA English DT Article; Proceedings Paper CT Conference on The Skeleton in the Hospital Closet - 20 Years Later: Malnutrition in Patients with GI Disease, Cancer and AIDS/10th World Congress of Gastroenterology CY OCT 01-07, 1994 CL LOS ANGELES, CA SP NUTRITION ID GASTROINTESTINAL SURGERY; NUTRITIONAL-STATUS; CLINICAL JUDGMENT; PROGNOSTIC INDEX; MEDICAL PATIENTS; IMMUNITY; IMPACT; RISK C1 UNIV TORONTO,ST MICHAELS HOSP,DEPT MED,TORONTO,ON M5B 1W8,CANADA. RP KINOSIAN, B (reprint author), UNIV PENN,VET AFFAIRS MED CTR,DEPT MED,MED SERV,PHILADELPHIA,PA 19104, USA. NR 24 TC 10 Z9 10 U1 0 U2 2 PU NUTRITION PI SYRACUSE PA PO BOX 920, SYRACUSE, NY 13210-0920 SN 0899-9007 J9 NUTRITION JI Nutrition PD MAR-APR PY 1995 VL 11 IS 2 SU S BP 196 EP 197 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA QY255 UT WOS:A1995QY25500003 PM 7626900 ER PT J AU RAMANUJAM, T LUCHI, M SCHUMACHER, HR ZWILLICH, S CHANG, CP CALLEGARI, PE VONFELDT, JM FANG, QO WEINER, DB WILLIAMS, WV AF RAMANUJAM, T LUCHI, M SCHUMACHER, HR ZWILLICH, S CHANG, CP CALLEGARI, PE VONFELDT, JM FANG, QO WEINER, DB WILLIAMS, WV TI DETECTION OF T-CELL RECEPTORS IN EARLY RHEUMATOID-ARTHRITIS SYNOVIAL TISSUE SO PATHOBIOLOGY LA English DT Article DE RHEUMATOID ARTHRITIS; T CELL RECEPTOR; POLYMERASE CHAIN REACTION; EARLY ARTHRITIS; SYNOVIAL TISSUE; SYNOVIAL BIOPSY ID EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; COLLAGEN-INDUCED ARTHRITIS; MYELIN BASIC-PROTEIN; MAJOR HISTOCOMPATIBILITY COMPLEX; V-BETA USAGE; ANTIGEN RECEPTOR; RESTRICTED HETEROGENEITY; CHAIN; GENES; ANTIBODIES AB Synovial tissue is rarely available from patients with early synovitis, with the exception of synovial biopsies, However, T cell populations early in the development of synovitis may be enriched in antigen-specific cells and critical to disease pathogenesis, To investigate the T cell repertoire in early synovitis, we utilized a PCR protocol for detection of T cell receptor (TCR) transcripts present in small amounts of synovial tissue. To expand the substrate for PCR, preamplification of cDNA was performed with a 3' constant region primer plus either a mixture of variable region primers or random hexanucleotides. Utilizing this method improved the sensitivity of detection, This technique is applied here to the analysis of TCR transcripts in synovial biopsies from individuals with early rheumatoid arthritis (RA) and non-RA synovitis, TCR alpha-chain transcripts were detectable in 5/5 RA and 4/4 non-RA specimens evaluated, with beta-chain transcripts detected in 4/5 early RA and 4/4 non-RA specimens evaluated, Confirmation of transcripts by sequencing of cloned PCR products verfied the specificity of amplification. The most frequently expressed TCR V region families in early RA synovitis were V alpha 11, V alpha 14, V alpha 28, V beta 7, V beta 9 and V beta 17. Several of these V regions have previously been implicated in studies of chronic RA synovitis, J alpha and J beta region usage was similar to that seen in chronic RA, and conserved N region motifs were apparent. We Early arthritis conclude that it is possible to detect TCR transcripts in small synovial biopsies Synovial tissue from individuals with early arthritis, This technique can be applied to studies Synovial biopsy of synovitis where limited quantities of synovial tissue are available. C1 UNIV PENN,SCH MED,DEPT MED,DIV RHEUMATOL,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,INST BIOTECHNOL & ADV MOLEC MED,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. CHILDRENS HOSP PHILADELPHIA,PHILADELPHIA,PA 19104. UNIV ROCHESTER,SCH MED,DEPT MED,RHEUMATOL SECT,ROCHESTER,MI. RI Weiner, David/H-8579-2014 NR 42 TC 9 Z9 9 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1015-2008 J9 PATHOBIOLOGY JI Pathobiology PD MAR-APR PY 1995 VL 63 IS 2 BP 100 EP 108 DI 10.1159/000163940 PG 9 WC Cell Biology; Pathology SC Cell Biology; Pathology GA RY038 UT WOS:A1995RY03800007 PM 8554698 ER PT J AU SILVA, JA LEONG, GB FERRARI, MM AF SILVA, JA LEONG, GB FERRARI, MM TI DELUSIONAL MISIDENTIFICATION OF HEALTH-CARE PROFESSIONALS SO PSYCHIATRIC QUARTERLY LA English DT Article ID CAPGRAS SYNDROME; SUBSTITUTION; PSYCHOSIS AB Delusional misidentification of others generally involves a person misidentifying others who are emotionally and geographically proximate to that person. Health care professionals may become the objects of delusional misidentification. The present study reviews and analyzes the anglophonic literature on published reports of misidentified health care professionals and adds two new cases. Relevant issues raised when patients misidentify health care professionals are explored, particularly the danger posed by these misidentification delusions. C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. CTR HLTH CARE SERV,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,SAN ANTONIO,TX 78284. UNIV CALIF LOS ANGELES,DEPT PSYCHIAT BIOBEHAV SCI,LOS ANGELES,CA 90024. RP SILVA, JA (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,PSYCHIAT SERV 116A,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 45 TC 3 Z9 3 U1 0 U2 0 PU HUMAN SCI PRESS INC PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 SN 0033-2720 J9 PSYCHIAT QUART JI Psychiatr. Q. PD SPR PY 1995 VL 66 IS 1 BP 51 EP 61 DI 10.1007/BF02238715 PG 11 WC Psychiatry SC Psychiatry GA QB192 UT WOS:A1995QB19200004 PM 7701020 ER PT J AU JARVIK, ME GROSS, TM ROSENBLATT, MR STEIN, RE AF JARVIK, ME GROSS, TM ROSENBLATT, MR STEIN, RE TI ENHANCED LEXICAL PROCESSING OF SMOKING STIMULI DURING SMOKING ABSTINENCE SO PSYCHOPHARMACOLOGY LA English DT Article DE ABSTINENCE; INTRUSIVE THOUGHTS; NICOTINE; PRIMING; SEMANTIC ACTIVATION; PERCEPTION; PROCESSING ID TOBACCO WITHDRAWAL; ANXIETY-STATES; THREAT CUES; NICOTINE; MEMORY; MOOD; BIAS AB In a group of heavy smokers, overnight abstinence from smoking facilitated the perception of briefly presented smoking words. Subjects in the nicotine-abstinent condition accurately identified significantly more smoking-related words than food-related or neutral words. However, a group tested in a non-abstinent condition showed no significant differences in ability to identify the three different word types. Smokers deprived of cigarettes were also significantly better able to categorize smoking words than non-abstinent subjects. These results demonstrate an abstinence-based facilitation of processing smoking-related stimuli at the semantic level, consistent with the hypothesis that smoking-related concepts are activated, or primed, during deprivation from nicotine. RP JARVIK, ME (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,WILSHIRE & SAWTELE BLVD,LOS ANGELES,CA 90073, USA. NR 23 TC 18 Z9 18 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD MAR PY 1995 VL 118 IS 2 BP 136 EP 141 DI 10.1007/BF02245831 PG 6 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA QR462 UT WOS:A1995QR46200008 PM 7617799 ER PT J AU MYERS, GA CONHAIM, RL ROSENFELD, DJ HARMS, BA AF MYERS, GA CONHAIM, RL ROSENFELD, DJ HARMS, BA TI EFFECTS OF PENTAFRACTION AND HETASTARCH PLASMA EXPANSION ON LUNG AND SOFT-TISSUE TRANSVASCULAR FLUID FILTRATION SO SURGERY LA English DT Article ID INCREASED VASCULAR PRESSURE; AWAKE SHEEP; HYPOPROTEINEMIA; RESUSCITATION; LYMPH; TRANSPORT; INFUSION; BALANCE; ALBUMIN; SHOCK AB Background. Hetastarch and pentafraction are high molecular weight starch solutions designed to augment plasma oncotic pressure. Although clinical utilization of hetastarch has been limited by reported coagulation abnormalities, pentafraction is a newer derivative that appears to have Jew adverse hemostatic effects. We examined the ability of pentafraction to modulate lung and soft tissue transvascular fluid filtration under hypoproteinemic conditions compared with hetastarch and Ringer's lactate (LR). Methods. Awake, protein-depleted sheep (n = 19) were prepared with lung and soft tissue lymph fistulas, and comparable infusions of 5% pentafraction (n = 6), 6% hetastarch (n = 6), or LR (n = 7) were administered Plasma and lymph samples were collected during 24-hour period to determine changes in protein concentrations, plasma-to-lymph oncotic gradients, and lung (Q(L)) and soft tissue (Q(S)) lymph flows. Results. Q(L) and Q(S) rose nearly twofold after protein depletion alone. LR infusion increased Q(L) and Q(S) to 8.7 +/- 1.7 and 3.1 +/- 0.6 times normoproteinemic baseline, respectively (p < 0.05). In contrast, hetastarch and pentafraction infusion limited the increase in (Q(L) to 4.2 +/- 1.1 and 4.0 +/- 0.8 times normoproteinemic baseline, respectively (p < 0.05 versus LR) and did not significantly increase as. Hetastarch and pentafraction infusions increase plasma oncotic pressure by nearly 6 mm Hg, which significantly widened the plasma-to-lymph oncotic pressure gradients above preinfusion baseline by 4.7 +/- 0.7 and 3.4 +/- 0.4 mm Hp in lung and 4.6 +/- 0.7 and 3.2 +/- 0.4 mm Hg in soft tissue, respectively (p < 0.05). Conclusions. Both hetastarch and pentafraction limit transvascular fluid filtration under hypoproteinemic conditions by augmenting plasma oncotic pressure and the plasma-to-lymph oncotic pressure gradient. Because of fewer adverse hemostatic effects pentafraction may be an improvement over current therapies in critical care fluid management. C1 UNIV WISCONSIN HOSP & CLIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,DEPT SURG,MADISON,WI 53792. UNIV WISCONSIN,DEPT SURG,MADISON,WI. FU NHLBI NIH HHS [HL46236] NR 27 TC 10 Z9 10 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0039-6060 J9 SURGERY JI Surgery PD MAR PY 1995 VL 117 IS 3 BP 340 EP 349 DI 10.1016/S0039-6060(05)80211-9 PG 10 WC Surgery SC Surgery GA QK689 UT WOS:A1995QK68900016 PM 7533333 ER PT J AU MOSER, R OBERLEY, TD DAGGETT, DA FRIEDMAN, AL JOHNSON, JA SIEGEL, FL AF MOSER, R OBERLEY, TD DAGGETT, DA FRIEDMAN, AL JOHNSON, JA SIEGEL, FL TI EFFECTS OF LEAD ADMINISTRATION ON DEVELOPING RAT-KIDNEY .1. GLUTATHIONE-S-TRANSFERASE ISOENZYMES SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID INTRANUCLEAR INCLUSION-BODIES; DIETARY CALCIUM; LIVER; EXPRESSION; INDUCTION; BINDING; NEPHROTOXICITY; RESISTANCE; PRESSURE; SUBUNITS AB The effects of acute and chronic exposure to lead on glutathione S-transferase (GST) isoforms were determined in developing kidney in the rat. The ontogeny of glutathione S-transferase isoforms was characterized as were the effects of depletion of dietary calcium on glutathione S-transferase isoform profiles in control and lead-treated rats, In the acute exposure experiments, rats of 14 and 50 days of age received three daily injections of lead acetate (114 mg/kg) and in the chronic exposure studies, rats received lead acetate at doses ranging from 50 to 500 ppm in their drinking water. Lead acetate administration in these chronic studies began 1 day after conception. Acute and chronic lead exposure had similar effects, causing increases in all but one glutathione S-transferase isoform (Yb-3); these increases were markedly exacerbated by dietary calcium depletion, In all lead paradigms, GST subunits Yb-1 and Yp showed the largest increases-greater than 25-fold in rats fed a low-calcium diet, GST subunit Yb-3 showed small increases in the 14-day acute lead and the 4 week low-calcium animals and did not increase in other groups. Lead-related increases in GSTs were partially reversed by transferring animals previously receiving lead to lead-free water for a 4-week period. Kidneys of rats fed the low-calcium diet did not have detectable GST Yk, but in rats on this low-calcium diet that received 500 ppm lead; this GST isoform was found at levels comparable to those in control rats fed lab chow. (C) 1995 Academic Press. Inc. C1 UNIV WISCONSIN,WAISMAN CTR,DEPT PEDIAT,MADISON,WI 53705. UNIV WISCONSIN,WAISMAN CTR,DEPT BIOMOLEC CHEM,MADISON,WI 53705. UNIV WISCONSIN,WAISMAN CTR,DEPT PATHOL,MADISON,WI 53705. UNIV WISCONSIN,WAISMAN CTR,CTR ENVIRONM TOXICOL,MADISON,WI 53705. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI 53705. FU NICHD NIH HHS [HD03352]; NIEHS NIH HHS [T32 ES07015]; NINDS NIH HHS [NS24669] NR 44 TC 27 Z9 30 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR PY 1995 VL 131 IS 1 BP 85 EP 93 DI 10.1006/taap.1995.1050 PG 9 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QK940 UT WOS:A1995QK94000011 PM 7878682 ER PT J AU OBERLEY, TD FRIEDMAN, AL MOSER, R SIEGEL, FL AF OBERLEY, TD FRIEDMAN, AL MOSER, R SIEGEL, FL TI EFFECTS OF LEAD ADMINISTRATION ON DEVELOPING RAT-KIDNEY .2. FUNCTIONAL, MORPHOLOGIC, AND IMMUNOHISTOCHEMICAL STUDIES SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article ID GLUTATHIONE-S-TRANSFERASE; MANGANESE SUPEROXIDE-DISMUTASE; SYRIAN-HAMSTER TISSUES; ANTIOXIDANT ENZYMES; CATALYTIC ACTIVITY; MESSENGER-RNAS; CELL-NUCLEUS; NEPHROPATHY; LOCALIZATION; ISOENZYMES AB The effects of chronic lead administration on renal function and cytoarchitecture and on the immunohistochemical localization of glutathione S-transferase (GST) isoenzymes were determined. Pregnant rats were given 250 ppm lead acetate in drinking water from conception until weaning and mothers and pups received 500 ppm of lead acetate from weaning until termination at either 3 or 7 weeks of age, Light and electron microscopic studies after 3 weeks of lead administration showed tubular injury with frequent mitoses noted in proximal tubular cells and, after 7 weeks of treatment, interstitial fibrosis, characteristic intranuclear inclusions, and tubular injury characterized by both nuclear and cytoplasmic pleomorphism. Rats treated with lead for 7 weeks showed significantly lower body weights and creatinine clearances than age-matched control animals, Immunohistochemical studies of glutathione transferase subunits in control rats showed unique isoform localization in each segment of the nephron; treatment with lead caused large increases in immunoreactive protein of Yc, Yk, Yb-1, and Yp GST subunits in proximal tubules, No increases in the antioxidant enzymes copper-zinc superoxide dismutase, catalase, and glutathione peroxidase were found in lead-treated rats, but there was a diffuse lead-related increase in immunoreactive protein for manganese superoxide dismutase throughout the renal cortex. Our results demonstrate large lead-induced increases of specific isoforms of glutathione S-transferase in specific kidney cell types and show that these increases preceded irreversible renal damage. (C) 1995 Academic Press, Inc. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL SERV,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT PATHOL,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT PEDIAT,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT BIOMOLEC CHEM,MADISON,WI 53705. UNIV WISCONSIN,CTR ENVIRONM TOXICOL,MADISON,WI 53705. UNIV WISCONSIN,WAISMAN CTR,MADISON,WI 53705. FU NICHD NIH HHS [HD03352]; NINDS NIH HHS [NS24969] NR 51 TC 45 Z9 46 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAR PY 1995 VL 131 IS 1 BP 94 EP 107 DI 10.1006/taap.1995.1051 PG 14 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QK940 UT WOS:A1995QK94000012 PM 7878683 ER PT J AU BLUMBERG, ML JOSEPH, AM FREEMAN, JR AF BLUMBERG, ML JOSEPH, AM FREEMAN, JR TI A STRATEGY FOR IMPROVING THE SUPERVISION AND PERFORMANCE OF MOONLIGHTING RESIDENTS SO ACADEMIC MEDICINE LA English DT Article ID PRACTICE GUIDELINES; HOUSE OFFICERS AB Background. In 1990 the Ambulatory Care Service of the Ralph H. Johnson Department of Veterans Affairs Medical Center, affiliated with the Medical University of South Carolina College of Medicine, developed written clinical protocols for the management of patients with certain high risk medical conditions. Method. Appropriateness of care was assessed by determining physician compliance to the protocols over a 24-month period (October 1990-September 1992). All physicians who did not comply received individualized feedback from the service chief. For the first 12 months, both staff physicians and moonlighting second- and third-year medical residents were assessed (a total of seven staff physicians and 20 residents participated in the study). For the second 12 months, only the residents were assessed. Results. The moonlighting residents were notably less consistent than the staff physicians in protocol compliance (95-100% for the staff physicians; 78-100% for the moonlighters). Additional interventions were then implemented to improve the moonlighters' utilization of the protocols. Moonlighters' compliance over the subsequent 12 months was less variable (mean compliance of 92%, SD, 3%, the first year versus 95%, SD, 8%, the second year). Conclusion. The strategy seemed to improve the supervision and performance of the moonlighting residents and promoted consistent delivery of high-quality outpatient care. C1 MED UNIV S CAROLINA,COLL MED,DEPT MED,CHARLESTON,SC. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,AMBULATORY CARE SERV,CHARLESTON,SC. NR 10 TC 4 Z9 4 U1 0 U2 1 PU HANLEY & BELFUS INC PI PHILADELPHIA PA 210 S 13TH ST, PHILADELPHIA, PA 19107 SN 1040-2446 J9 ACAD MED JI Acad. Med. PD FEB PY 1995 VL 70 IS 2 BP 155 EP 157 DI 10.1097/00001888-199502000-00022 PG 3 WC Education, Scientific Disciplines; Health Care Sciences & Services SC Education & Educational Research; Health Care Sciences & Services GA QH465 UT WOS:A1995QH46500025 PM 7865044 ER PT J AU HARRIS, RA PROCTOR, WR MCQUILKIN, SJ KLEIN, RL MASCIA, MP WHATLEY, V WHITING, PJ DUNWIDDIE, TV AF HARRIS, RA PROCTOR, WR MCQUILKIN, SJ KLEIN, RL MASCIA, MP WHATLEY, V WHITING, PJ DUNWIDDIE, TV TI ETHANOL INCREASES GABA(A) RESPONSES IN CELLS STABLY TRANSFECTED WITH RECEPTOR SUBUNITS SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE GABA; ETHANOL; CELL CULTURE; TRANSFECTION GABA; RECEPTORS ID GAMMA-AMINOBUTYRIC-ACID; GATED CHLORIDE CHANNELS; XENOPUS OOCYTES; MESSENGER-RNAS; ION CHANNELS; LONG-SLEEP; RAT-BRAIN; NEURONS; POTENTIATION; SITES AB Ethanol enhancement of GABA(A) receptor function has been found in some, but not all, studies. These results suggest the existence of ethanol-sensitive and -resistant receptors that may differ in subunit composition, although methodological differences (e,g., Cl-36(-) flux versus membrane currents) could also contribute to the different results. To examine these possibilities, we used mouse L(tk(-)) cells stably transfected with alpha(1) + beta(1) or alpha(1) + beta(1) + gamma(2L) GABA(A) receptor subunit DNAs and compared Cl-36(-) flux with whole-cell, patch-clamp measurements of GABA(A) receptor function. Both techniques detected a similar modulation of the GABA receptor by ethanol, flunitrazepam, and pentobarbital. The potentiating action of ethanol required the gamma-subunit and was maximal at a concentration of 10 mM, Similar ethanol potentiation was obtained with brief (20 msec) or long (2 sec) applications of GABA. Analysis of data obtained from individual cells expressing alpha(1) beta(1-)gamma(2L) subunits showed considerable variability in sensitivity to ethanol, particularly with concentrations of 30 and 100 mM, Ethanol potentiated GABA action if the cells were grown on coverslips coated with polylysine, but had no effect on GABA(A) receptors of cells grown on uncoated coverslips. Thus, ethanol action was influenced by the growth matrix. Taken together, these data indicate that a gamma-subunit is necessary, but not sufficient, for ethanol sensitivity in this cell system. We suggest that posttranslational processing, particularly receptor phosphorylation, may also be important and that stably transfected cells will be useful in elucidating these events. C1 UNIV COLORADO, SCH MED, DENVER VA MED CTR, ALCOHOL RES CTR, DENVER, CO USA. MERCK SHARP & DOHME LTD, NEUROSCI RES LABS, HARLOW CM20 2QR, ESSEX, ENGLAND. RP UNIV COLORADO, HLTH SCI CTR,SCH MED,DEPT PHARMACOL,C236, 4200 E 9TH AVE, DENVER, CO 80262 USA. NR 29 TC 102 Z9 104 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-6008 EI 1530-0277 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD FEB PY 1995 VL 19 IS 1 BP 226 EP 232 DI 10.1111/j.1530-0277.1995.tb01496.x PG 7 WC Substance Abuse SC Substance Abuse GA QG625 UT WOS:A1995QG62500036 PM 7771653 ER PT J AU GRACEFFO, MA OROURKE, RA HIBNER, C BOULET, AJ AF GRACEFFO, MA OROURKE, RA HIBNER, C BOULET, AJ TI THE TIME-COURSE AND RELATION OF POSITIVE SIGNAL-AVERAGED ELECTROCARDIOGRAMS BY TIME-DOMAIN AND SPECTRAL TEMPORAL MAPPING ANALYSES AFTER INFARCTION SO AMERICAN HEART JOURNAL LA English DT Article ID ACUTE MYOCARDIAL-INFARCTION; SUSTAINED VENTRICULAR-TACHYCARDIA; CORONARY-ARTERY DISEASE; HIGH-FREQUENCY COMPONENTS; BUNDLE-BRANCH BLOCK; LATE POTENTIALS; PROGNOSTIC-SIGNIFICANCE; ARRHYTHMIC EVENTS; QUANTITATIVE-ANALYSIS; NATURAL-HISTORY AB We evaluated the time course of development of positive signal-averaged electrocardiograms (SA-ECGs) by time-domain and Spectral Temporal Mapping(TM) (STM) analyses after myocardial infarction in 88 patients without bundle branch block, The incidence of positive SA-ECGs by time-domain analysis peaked at 4 to 8 weeks postinfarction whereas the peak incidence by STM analysis varied from 4 days to 4 to 10 months postinfarction. Positive time-domain SA-ECGs demonstrated a significantly reduced factor of normality (NF) compared with negative time-domain SA-ECGs by X, Z, or vector SIM analyses, but marked overlap was present for the standard deviations of positive and negative SA-ECGs in all STM leads. Chi square analysis demonstrated a significant correlation only between X-lead STM analysis and time-domain analysis; however, the two methods were markedly discordant. Although there is a statistically significant relation between time-domain and STM analyses of SA-ECGs, the two analyses are not clinically interchangeable. C1 UNIV TEXAS,HLTH SCI CTR,DIV CARDIOL,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX. FU NHLBI NIH HHS [5 T32 HL07350] NR 52 TC 8 Z9 8 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0002-8703 J9 AM HEART J JI Am. Heart J. PD FEB PY 1995 VL 129 IS 2 BP 238 EP 251 DI 10.1016/0002-8703(95)90004-7 PG 14 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA QE518 UT WOS:A1995QE51800005 PM 7832095 ER PT J AU ZHANG, RS GUTH, PH SCREMIN, OU CHAUDHURI, G AF ZHANG, RS GUTH, PH SCREMIN, OU CHAUDHURI, G TI H-2 GAS CLEARANCE TECHNIQUE FOR SEPARATING RAT UTERINE BLOOD-FLOW INTO ENDOMETRIAL AND MYOMETRIAL COMPONENTS SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY LA English DT Article DE OVARIECTOMIZED RAT; IODO[C-14]ANTIPYRINE AUTORADIOGRAPHY; ENDOMETRIAL BLOOD FLOW; MYOMETRIAL BLOOD FLOW; GASTRIC MUCOSAL BLOOD FLOW; 17-BETA-ESTRADIOL; PENTAGASTRIN; PHENYLEPHRINE ID OOPHORECTOMIZED EWES; ESTRADIOL AB The H-2 gas clearance technique was employed to measure uterine blood flow (UBF) in ovariectomized rats. A needle-type platinum electrode (125 mu m diam) was inserted into the rat uterine wall to measure the tissue blood flow surrounding the electrode. The electrode can be placed in individual layers of the uterus to measure the endometrial blood flow (EBF) or the myometrial blood flow (MBF). By use of this technique, baseline EBF and MBF were 37.8 +/- 3.53 (n = 21) and 47.2 +/- 4.56 (n = 5) ml.min(-1).100 g(-1), respectively, with an EBF/MBF ratio of 0.8. Intravenous bolus injection of 17 beta-estradiol (1 mu g/kg) induced a significant increase in UBF. Phenylephrine, an alpha-adrenergic receptor agonist, reduced UBF. In some animals, a second platinum electrode was used to measure gastric mucosal blood flow simultaneously with UBF. While 17 beta-estradiol selectively increased UBF, pentagastrin selectively increased gastric mucosal blood flow. To further validate the baseline UBF distribution between endometrial and myometrial layers, iodo[C-14]antipyrine autoradiography was employed. With the iodo[C-14]antipyrine technique, the EBF/MBF ratio was 0.91 +/- 0.07 (n = 5), which is similar to that obtained with the H-2 gas clearance technique. C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT OBSTET & GYNECOL MOLEC & MED PHARMACOL, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT PHYSIOL, LOS ANGELES, CA 90024 USA. W LOS ANGELES VET AFFAIRS MED CTR, CTR ULCER RES & EDUC, LOS ANGELES, CA 90024 USA. FU NHLBI NIH HHS [HL-46843] NR 40 TC 7 Z9 7 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6119 J9 AM J PHYSIOL-REG I JI Am. J. Physiol.-Regulat. Integr. Compar. Physiol. PD FEB PY 1995 VL 268 IS 2 BP R569 EP R575 PG 7 WC Physiology SC Physiology GA QF753 UT WOS:A1995QF75300034 PM 7864254 ER PT J AU ASCH, DA HANSENFLASCHEN, J LANKEN, PN AF ASCH, DA HANSENFLASCHEN, J LANKEN, PN TI DECISIONS TO LIMIT OR CONTINUE LIFE-SUSTAINING TREATMENT BY CRITICAL CARE PHYSICIANS IN THE UNITED-STATES - CONFLICTS BETWEEN PHYSICIANS PRACTICES AND PATIENTS WISHES SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article ID ILL PATIENTS; FUTILITY; ATTITUDES; SUPPORT; EUTHANASIA; WITHDRAWAL; POLICY AB We surveyed a national sample of 879 physicians practicing in adult intensive care units in the United States, in order to determine their practices with regard to limiting life-sustaining medical treatment, and particularly their decisions to continue or forgo life support without the consent or against the wishes of patients or surrogates. Virtually all of the respondents (96%) have withheld and withdrawn life-sustaining medical treatment on the expectation of a patient's death, and most do so frequently in the course of a year. Many physicians continue life-sustaining treatment despite patient or surrogate wishes that it be discontinued (34%), and many unilaterally withhold (83%) or withdraw (82%) life-sustaining treatment that they judge to be futile. Some of these decisions are made without the knowledge or consent of patients or their surrogates, and some are made over their objections. We conclude that physicians do not reflexively accept requests by patients or surrogates to limit or continue life-sustaining treatment, but place these requests alongside a collection of other factors, including assessments of prognosis and perceptions of other ethical, legal, and policy guidelines. While debate continues about the ethical and legal foundations of medical futility, our results suggest that most critical care physicians are incorporating some concept of medical futility into decision making at the bedside. C1 UNIV PENN, LEONARD DAVIS INST HLTH ECON, PHILADELPHIA, PA 19104 USA. UNIV PENN, SCH MED, DIV PULM & CRIT CARE MED, PHILADELPHIA, PA 19104 USA. RP ASCH, DA (reprint author), UNIV PENN, SCH MED,VET AFFAIRS MED CTR,DIV GEN INTERNAL MED, 317 RALSTON PENN CTR, 3615 CHESTNUT ST, PHILADELPHIA, PA 19104 USA. OI Hansen-Flaschen, John/0000-0003-1989-7244 NR 33 TC 177 Z9 180 U1 0 U2 3 PU AMER THORACIC SOC PI NEW YORK PA 61 BROADWAY, FL 4, NEW YORK, NY 10006 USA SN 1073-449X EI 1535-4970 J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD FEB PY 1995 VL 151 IS 2 BP 288 EP 292 PG 5 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA QF547 UT WOS:A1995QF54700008 PM 7842181 ER PT J AU ZITTEL, TT VONELM, B TEICHMANN, RK RABOULD, HE BECKER, HD AF ZITTEL, TT VONELM, B TEICHMANN, RK RABOULD, HE BECKER, HD TI CHOLECYSTOKININ IS PARTLY RESPONSIBLE FOR REDUCED FOOD-INTAKE AND BODY-WEIGHT LOSS AFTER TOTAL GASTRECTOMY IN RATS SO AMERICAN JOURNAL OF SURGERY LA English DT Article ID ROUX-Y ESOPHAGOJEJUNOSTOMY; RECEPTOR-BINDING SITES; CCK-A RECEPTORS; NUTRITIONAL-STATUS; BRAIN; RECONSTRUCTION; ANTAGONIST; L-365,260; MALABSORPTION; CONSEQUENCES AB PURPOSE: To determine the cause of body weight loss after total gastrectomy. METHODS: We evaluated the acute effect of exogenous cholecystokinin (CCK) on food intake and the chronic effect of CCK receptor blockade on food intake and body weight after total gastrectomy in rats. RESULTS: Exogenous CCK significantly reduced food intake in gastrectomized rats; this was blocked by administration of a CCK-A but not a CCK-B receptor antagonist. Chronic treatment with a CCK-A or CCK-B receptor antagonist after total gastrectomy in rats significantly increased postoperative food intake and body weight. CONCLUSION: Our data indicate that endogenous CCK is partly responsible for reduced food intake and body weight loss after total gastrectomy in rats. C1 UNIV TUBINGEN HOSP,DEPT ABDOMINAL & TRANSPLANTAT SURG,TUBINGEN,GERMANY. W LOS ANGELES VET AFFAIRS MED CTR,CTR GASTROENTER BIOL,CURE,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. NR 50 TC 15 Z9 15 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD FEB PY 1995 VL 169 IS 2 BP 265 EP 270 DI 10.1016/S0002-9610(99)80148-2 PG 6 WC Surgery SC Surgery GA QE637 UT WOS:A1995QE63700014 PM 7840391 ER PT J AU CANVER, CC ARMSTRONG, VM NICHOLS, RD MENTZER, RM AF CANVER, CC ARMSTRONG, VM NICHOLS, RD MENTZER, RM TI COLOR-FLOW DUPLEX ULTRASOUND ASSESSMENT OF INTERNAL THORACIC ARTERY GRAFT AFTER CORONARY-BYPASS SO ANNALS OF THORACIC SURGERY LA English DT Article ID MAMMARY ARTERY; REACTIVE HYPEREMIA AB In an effort to develop a noninvasive method to evaluate now characteristics of the internal thoracic artery (ITA) graft after coronary artery bypass grafting, we performed color-now duplex ultrasound studies of the right and left ITAs of 42 patients before and 5 weeks after bypass grafting. The ITA was visualized with a duplex scanner (5.0-MHz probe) through the first or second intercostal space. We recorded the diameter, peak systolic velocity, and end-diastolic velocity for each patient. Preoperative measurements of the native ITAs were obtained easily in all patients. Postoperatively, the unused right ITA was seen in all patients. Postoperative visualization of the left ITA graft was adequate to make reliable measurements in 40 patients (95%). Postoperative end-diastolic velocities of the unused right ITA and the left ITA graft were markedly higher than the preoperative end-diastolic velocities of the native ITAs (p < 0.001). Whereas there was a marked increase in the peak systolic velocity of the postoperative unused right ITA (p < 0.05), the postoperative peak systolic velocity of the left ITA graft was significantly lower than the preoperative value (p < 0.001). We conclude that postoperative visualization of the left ITA graft is possible with the use of color-flow duplex ultrasound. Ultrasonic surveillance of postoperative ITAs may reveal ITA graft velocity abnormalities before overt graft failure is manifested in the patient who has undergone coronary artery bypass grafting. C1 UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,SCH MED,CARDIOTHORAC SURG SECT,MADISON,WI. NR 11 TC 15 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0003-4975 J9 ANN THORAC SURG JI Ann. Thorac. Surg. PD FEB PY 1995 VL 59 IS 2 BP 389 EP 392 DI 10.1016/0003-4975(94)00842-U PG 4 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA QF299 UT WOS:A1995QF29900021 PM 7847953 ER PT J AU MANN, D SCHUMACHER, HR AF MANN, D SCHUMACHER, HR TI PSEUDOSEPTIC INFLAMMATORY KNEE EFFUSION CAUSED BY PHAGOCYTOSIS OF SICKLED ERYTHROCYTES AFTER FRACTURE INTO THE KNEE-JOINT SO ARTHRITIS AND RHEUMATISM LA English DT Note ID CELL DISEASE; ARTHRITIS AB A 57-year-old black man with sickle cell disease was admitted to the hospital because of a painful crisis. After a fall with a fracture into the right knee joint, he developed an acutely painful, swollen knee. Synovial fluid from the right knee showed leukocyte counts of up to 154,000/mm(3) and was negative for urate and calcium pyrophosphate dihydrate crystals. Gram stains and cultures were negative, Some sickled red cells were seen by light microscopy; electron microscopy revealed crystallike arrays of sickled hemoglobin tactoids in erythrocytes which were enfolded and phagocytized by the cells of the synovial fluid. We suggest that this phagocytosis of sickled red cells is the likely cause for the otherwise unexplained inflammation, C1 DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. UNIV PENN,SCH MED,PHILADELPHIA,PA 19104. OI Mann, Douglas /0000-0002-2516-0145 NR 10 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQUARE, PHILADELPHIA, PA 19106 SN 0004-3591 J9 ARTHRITIS RHEUM JI Arthritis Rheum. PD FEB PY 1995 VL 38 IS 2 BP 284 EP 287 DI 10.1002/art.1780380219 PG 4 WC Rheumatology SC Rheumatology GA QF433 UT WOS:A1995QF43300018 PM 7848320 ER PT J AU SILVA, JA LEONG, GB AF SILVA, JA LEONG, GB TI VISUAL-PERCEPTUAL ABNORMALITIES IN DELUSIONAL MISIDENTIFICATION SO CANADIAN JOURNAL OF PSYCHIATRY-REVUE CANADIENNE DE PSYCHIATRIE LA English DT Note ID CAPGRAS DELUSION; FACE AB Delusional misidentification syndromes are often found in conjunction with visual-perceptual abnormalities. These abnormalities appear to be independent of visual hallucinations and are illusory in nature. A case of delusional misidentification with visual-perceptual abnormalities is presented. Cognitive and visual phenomenology suggests that careful recording of visual abnormalities may shed light on the relation between delusional misidentification and abnormal visual cognition. C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. UNIV CALIF LOS ANGELES,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA. RP SILVA, JA (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,PSYCHIAT SERV 116A,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 24 TC 11 Z9 11 U1 0 U2 0 PU CANADIAN PSYCHIATRIC ASSOC PI OTTAWA PA SUITE 200, 237 ARGYLE AVE, OTTAWA ON K2P 1B8, CANADA SN 0706-7437 J9 CAN J PSYCHIAT JI Can. J. Psychiat.-Rev. Can. Psychiat. PD FEB PY 1995 VL 40 IS 1 BP 6 EP 8 PG 3 WC Psychiatry SC Psychiatry GA QX508 UT WOS:A1995QX50800004 PM 7874682 ER PT J AU RAWSON, NE BRANS, JG LOWRY, LD TEETER, JH RESTREPO, D AF RAWSON, NE BRANS, JG LOWRY, LD TEETER, JH RESTREPO, D TI 2ND MESSENGER DEPENDENCE OF CALCIUM RESPONSES IN ISOLATED MAMMALIAN OLFACTORY NEURONS - ONE PATHWAY OR 2 SO CHEMICAL SENSES LA English DT Meeting Abstract ID ODORANTS; CA2+ C1 MONELL CHEM SENSES CTR,PHILADELPHIA,PA 19104. THOMAS JEFFERSON UNIV,PHILADELPHIA,PA 19107. VET AFFAIRS MED CTR,PHILADELPHIA,PA. UNIV PENN,PHILADELPHIA,PA 19104. NR 4 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0379-864X J9 CHEM SENSES JI Chem. Senses PD FEB PY 1995 VL 20 IS 1 BP 157 EP 158 PG 2 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA QQ130 UT WOS:A1995QQ13000178 ER PT J AU VIRELLA, G MIRONOVA, M LOPESVIRELLA, MF AF VIRELLA, G MIRONOVA, M LOPESVIRELLA, MF TI COMPARING ASSAYS OF ANTIBODIES TO MODIFIED LOW-DENSITY LIPOPROTEINS SO CLINICAL CHEMISTRY LA English DT Letter C1 RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC 29403. MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL,CHARLESTON,SC. MED UNIV S CAROLINA,DEPT MICROBIOL & IMMUNOL,CHARLESTON,SC 29425. FU NHLBI NIH HHS [HL46815] NR 8 TC 6 Z9 6 U1 0 U2 0 PU AMER ASSOC CLINICAL CHEMISTRY PI WASHINGTON PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 SN 0009-9147 J9 CLIN CHEM JI Clin. Chem. PD FEB PY 1995 VL 41 IS 2 BP 324 EP 325 PG 2 WC Medical Laboratory Technology SC Medical Laboratory Technology GA QG652 UT WOS:A1995QG65200030 PM 7874792 ER PT J AU HASEGAWA, H KANNER, AM AF HASEGAWA, H KANNER, AM TI SEIZURE CONTROL AFTER CHRONIC PHARMACOTHERAPY IN EPILEPTIC DISORDERS BEGINNING AFTER 40 YEARS OF AGE SO CLINICAL NEUROPHARMACOLOGY LA English DT Review DE ADULT ONSET EPILEPSY; MONOTHERAPY; POLYTHERAPY; CARBAMAZEPINE; PHENOBARBITAL; PRIMIDONE; PHENYTOIN ID STROKE AB Few studies have been published on the pharmacologic response to treatment of patients whose seizures begin after 40 years of age. The purpose of this study was to assess the impact of chronic pharmacotherapy on the seizure control of such patients. We retrospectively studied the seizure frequency recorded during a 12-month period in a group of 94 outpatients whose seizure disorders began after 40 years of age (median age of seizure onset 56.5 years) and who had been treated with anticonvulsant medication for a median period of 6 years (range 18 months to 12 years). We assessed the relationship between the patients' seizure frequency during the last 12 months of treatment using (a) the present and previously prescribed pharmacologic regimens, (b) anticonvulsant blood levels of present regimen, (c) etiology and duration of seizure disorder, (d) age at onset of seizures, and (e) presence of electrographic (EEG) and neuroradiologic abnormalities. We only identified side effects occurring at blood levels within or below the drug's therapeutic range. Seventy-eight patients (83%) were seizure free during the last 12 months of treatment, 11 (11%) had rare seizures, and five (6%) had more than four seizures per year. Seizure frequency was not affected by duration and etiology of seizure disorder, age at onset of seizures, seizure type, neuroradiologic or electroencephalographic abnormalities, and present or previously prescribed pharmacologic regimens, Persistent side effects were reported in seven of 76 (9%) monotherapy regimens and in two of 12 (17%) polytherapy regimens. Our data suggest that seizures beginning after the age of 40 have a favorable prognosis after chronic pharmacotherapy. C1 RUSH PRESBYTERIAN ST LUKES MED CTR,RUSH EPILEPSY CTR,CHICAGO,IL 60612. RUSH PRESBYTERIAN ST LUKES MED CTR,DEPT NEUROL SCI,CHICAGO,IL 60612. WILLIAM S MIDDLETON MEM VET ADM MED CTR,NEUROL SERV,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,FRANCIS M FORESTER EPILEPSY CTR,MADISON,WI. UNIV WISCONSIN,DEPT NEUROL,MADISON,WI. NR 23 TC 3 Z9 3 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0362-5664 J9 CLIN NEUROPHARMACOL JI Clin. Neuropharmacol. PD FEB PY 1995 VL 18 IS 1 BP 13 EP 22 DI 10.1097/00002826-199502000-00002 PG 10 WC Clinical Neurology; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA QF316 UT WOS:A1995QF31600002 PM 8665530 ER PT J AU SKINNER, MH KOSHSUBER, J VAN, B AF SKINNER, MH KOSHSUBER, J VAN, B TI USE OF A COMPUTER-PROVIDED ALERT MESSAGE TO IMPROVE THERAPEUTIC DRUG-MONITORING SO CLINICAL PHARMACOLOGY & THERAPEUTICS LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,DIV CLIN PHARM,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,QUAL IMPROVEMENT SERV,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SERV PHARM,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0009-9236 J9 CLIN PHARMACOL THER JI Clin. Pharmacol. Ther. PD FEB PY 1995 VL 57 IS 2 BP 192 EP 192 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA QJ312 UT WOS:A1995QJ31200227 ER PT J AU JAFFA, AA RUST, PF MAYFIELD, RK AF JAFFA, AA RUST, PF MAYFIELD, RK TI KININ, A MEDIATOR OF DIABETES-INDUCED GLOMERULAR HYPERFILTRATION SO DIABETES LA English DT Article ID MELLITUS; KALLIKREIN; RATS; INSULIN; PROSTAGLANDIN; RENIN; HYPERTENSION; LOCALIZATION; EXPRESSION; HORMONES AB Renal kallikrein is increased in diabetic patients and streptozotocin (STZ)-induced diabetic rats with hyperfiltration. Chronic inhibition of renal kallikrein reduces glomerular filtration rate (GFR) and renal plasma flow (RPF) in hyperfiltering Sn-induced diabetic rats, To investigate whether these actions of kallikrein and its inhibition are kinin-mediated, we used a B-2-kinin receptor antagonist (BKA). In STZ-induced diabetic rats with hyperfiltration, renal kallikrein excretion rate was significantly increased (P less than or equal to 0.01), and kinin excretion rate was increased 57%, as compared with control rats. Left kidney GFR and RPF were measured before and during a 40-min infusion of BKA (0.5 mu g kg(-1).min(-1)) or vehicle. Infusion of the kinin receptor antagonist reduced the GFR and RPF significantly. GFR was reduced by 18%, hom an average baseline value of 2.07 +/- 0.11 to 1.70 +/- 0.06 ml/min, P less than or equal to 0.001 (means +/- SE). RPF was reduced by 25%, hom 6.74 +/- 0.38 to 5.06 +/- 0.17 ml/min, P less than or equal to 0.001. Total renal vascular resistance was significantly increased during BKA infusion, P less than or equal to 0.001. Vehicle infusion for the same period had no significant effect on GFR, RPF, or renal vascular resistance. These findings further support the hypothesis that increased renal production of kinins contributes to the renal vasodilation of diabetes. C1 MED UNIV S CAROLINA,DEPT PHARMACOL,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT BIOMETRY,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. RP JAFFA, AA (reprint author), MED UNIV S CAROLINA,DEPT MED,DIV ENDOCRINOL DIABET & METAB,171 ASHLEY AVE,CHARLESTON,SC 29425, USA. NR 28 TC 50 Z9 51 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD FEB PY 1995 VL 44 IS 2 BP 156 EP 160 DI 10.2337/diabetes.44.2.156 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QD999 UT WOS:A1995QD99900005 PM 7859934 ER PT J AU JONES, RN SADER, HS ERWIN, ME ANDERSON, SC ALDRIDGE, KA ALLEN, S ANHALT, J APPELBAUM, P ARRINGTON, KL AYERS, L BAKER, C BEAVIS, K BERGER, J BERTHOLD, G BIRNBAUM, M BOYLE, J BRECHER, S BRECKENRIDGE, R BROWN, W BRUCKNER, D CARROLL, K CHAUDHARY, S CLEARY, T COCKERILL, F COYLE, M CRAWFORD, V DALTON, H DOERN, G EDBERG, S GELFAND, M GERLACH, EH GOODMAN, N GORZYNSKI, E GREEN, P GROSCHEL, D HANFF, P HANNA, B HARRELL, L HAUGEN, T HEAGREY, M HUMPHRIES, J ISENBERG, H JENKINS, S JONES, E JORGENSEN, J KAUFFMAN, C KEISER, J KOCKA, F KOMINOS, S LEVISON, M LOCKWOOD, W MANOS, J MARTIN, W MCLAUGHLIN, J METCHOCK, B MICKELSEN, P MOODY, J MORELLO, J MOSTOW, S MOTYL, M MURRAY, B MURRAY, PR NOLTE, F PARK, C PEZZLO, M PRICE, M PUSCSH, A REED, K REIMER, L RINALDI, M RISTUCCIA, A ROBINSON, A ROSATI, L SAUBOLLE, M SCHALHAB, J SCHULTZ, G SCHWALBE, R SEWELL, D SHALES, D SIERRA, M SINNOTT, J SLIFKIN, M SMITH, L SPIEGEL, C STANECK, J STEELEMOORE, L STEIN, G STEVENS, D STRATTON, C TAN, J THOMAS, J WAITES, K WEINSTEIN, M WELCH, W WIN, W WOODS, G WRIGHT, L YUNGBLUTH, M ZWADYK, P AF JONES, RN SADER, HS ERWIN, ME ANDERSON, SC ALDRIDGE, KA ALLEN, S ANHALT, J APPELBAUM, P ARRINGTON, KL AYERS, L BAKER, C BEAVIS, K BERGER, J BERTHOLD, G BIRNBAUM, M BOYLE, J BRECHER, S BRECKENRIDGE, R BROWN, W BRUCKNER, D CARROLL, K CHAUDHARY, S CLEARY, T COCKERILL, F COYLE, M CRAWFORD, V DALTON, H DOERN, G EDBERG, S GELFAND, M GERLACH, EH GOODMAN, N GORZYNSKI, E GREEN, P GROSCHEL, D HANFF, P HANNA, B HARRELL, L HAUGEN, T HEAGREY, M HUMPHRIES, J ISENBERG, H JENKINS, S JONES, E JORGENSEN, J KAUFFMAN, C KEISER, J KOCKA, F KOMINOS, S LEVISON, M LOCKWOOD, W MANOS, J MARTIN, W MCLAUGHLIN, J METCHOCK, B MICKELSEN, P MOODY, J MORELLO, J MOSTOW, S MOTYL, M MURRAY, B MURRAY, PR NOLTE, F PARK, C PEZZLO, M PRICE, M PUSCSH, A REED, K REIMER, L RINALDI, M RISTUCCIA, A ROBINSON, A ROSATI, L SAUBOLLE, M SCHALHAB, J SCHULTZ, G SCHWALBE, R SEWELL, D SHALES, D SIERRA, M SINNOTT, J SLIFKIN, M SMITH, L SPIEGEL, C STANECK, J STEELEMOORE, L STEIN, G STEVENS, D STRATTON, C TAN, J THOMAS, J WAITES, K WEINSTEIN, M WELCH, W WIN, W WOODS, G WRIGHT, L YUNGBLUTH, M ZWADYK, P TI EMERGING MULTIPLY RESISTANT ENTEROCOCCI AMONG CLINICAL ISOLATES .1. PREVALENCE DATA FROM 97 MEDICAL-CENTER SURVEILLANCE STUDY IN THE UNITED-STATES SO DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE LA English DT Article ID LACTAMASE-PRODUCING ENTEROCOCCI; VANCOMYCIN RESISTANCE; ANTIMICROBIAL SUSCEPTIBILITIES; GLYCOPEPTIDE RESISTANCE; FAECIUM D366; AMPICILLIN; FAECALIS; PENICILLIN; INFECTIONS; ANTIBIOTICS AB To assess the evolving problem of therapeutic drug resistances among enterococci, we organized a comprehensive national (United States) surveillance trial using 99 recruited microbiology laboratories in 48 of the 49 contiguous stares or districts. AIL but two sites completed the protocol that generated information from nearly 2000 enterococci, usually isolated from blood cultures. All strains were speciated by the same method (API 20S) and were susceptibility tested by three methods (broth microdilution, disk diffusion, and Etest) against ampicillin, penicillin, vancomycin, teicoplanin, gentamicin, and streptomycin. Strains resistant to a glycopeptide or penicillin, or possessing high-level aminoglycoside resistance were referred to the monitor's laboratory for validation and additional susceptibility testing against other alternative antimicrobial agents. The most common species were Enterococcus faecalis and Enterococcus faecium. However, antimicrobial resistance occurred most often among the E. faecium isolates. Twenty-three percent of participant centers (22 sites) reported 87 vancomycin-resistant isolates, which accounts for 4.4% of the isolates evaluated. A recent audit (March 1994) of the laboratories not reporting vancomycin resistance during the study interval (October-December 1992) revealed that 61% of sites have now recognized these strains, a threefold increase in 12-15 months. Teicoplanin remained active against 28% (Van B phenotype) of vancomycin-resistant enterococci (10 E. faecalis, 13 E. faecium, and one Enterococcus spp.). Ampicillin-resistant beta-lactamase-positive strains were found only at one medical center (two strains, 0.2% of referred or validated strains); however, ampicillin-resistant strains represented 12% of all enterococcal, bur nearly 60% of E. faecium strains. Aminoglycoside resistance was: gentamicin 27% and streptomycin 36% of strains. The susceptibility to alternative drugs was: ciprofloxacin 25%, erythromycin 3%, trimethoprim/sulfamethoxazole 22%, and spectinomycin 97%. All National Committee for Clinical Laboratory Standards tests and interpretive criteria performed well. Other drugs worthy of therapeutic consideration include chloramphenicol, tetracyclines (especially doxycycline), novobiocin, trospectomycin or kanamycin as a co-drug, and some newer fluoroquinolones (sparfloxacin and clinafloxacin). Because of this rapidly evolving problem of drug-resistant invasive enterococcal infections, new alternative combination regimens require immediate consideration for structured clinical trials. C1 MILTON S HERSHEY MED CTR, HERSHEY, PA USA. OHIO STATE UNIV HOSP, COLUMBUS, OH USA. CTR DIS CONTROL, ATLANTA, GA USA. THOMAS JEFFERSON UNIV, PHILADELPHIA, PA USA. ST BARNABAS HOSP, BRONX, NY USA. NEBRASKA METHODIST HOSP, OMAHA, NE USA. BROOKLYN HOSP CTR, BROOKLYN, NY USA. CABRINI MED CTR, MALVERN, VIC, AUSTRALIA. VET ADM MED CTR, BOSTON, MA USA. NICHOLS INST, LOMA LINDA, CA USA. WSU, CTR HLTH, DETROIT, MI USA. UNIV CALIF LOS ANGELES, LOS ANGELES, CA USA. UNIV UTAH, MED CTR, SALT LAKE CITY, UT 84112 USA. ST JOHNS HOSP, LONDON, ENGLAND. JACKSON MEM HOSP, MIAMI, FL USA. MAYO CLIN & MAYO FDN, ROCHESTER, MN USA. HARBORVIEW MED CTR, SEATTLE, WA USA. VIRGINIA COMMONWEALTH UNIV MED COLL VIRGINIA, RICHMOND, VA USA. UNIV MASSACHUSETTS, MED CTR, AMHERST, MA 01003 USA. YALE NEW HAVEN MED CTR, NEW HAVEN, CT USA. ST FRANCIS REG MED CTR, WICHITA, KS USA. UNIV KENTUCKY, COLL MED, LEXINGTON, KY 40506 USA. VET ADM MED CTR, BUFFALO, NY USA. HITCHCOCK MED CTR, HANOVER, NH USA. UNIV VIRGINIA, HLTH SCI CTR, CHARLOTTESVILLE, VA 22903 USA. BETH ISRAEL HOSP, BOSTON, MA USA. BELLEVUE HOSP, NEW YORK, NY USA. DUKE UNIV, MED CTR, DURHAM, NC 27706 USA. VET ADM MED CTR, IOWA CITY, IA USA. BOZEMAN DEACONESS HOSP, BOZEMAN, MT USA. UNIV MISSISSIPPI, MED CTR, UNIVERSITY, MS 38677 USA. LONG ISL JEWISH MED CTR, NEW HYDE PK, NY USA. ASSOCIATED PATHOL LABS, LAS VEGAS, NV USA. UNIV TEXAS, HLTH SCI CTR, SAN ANTONIO, TX USA. VET ADM MED CTR, ANN ARBOR, MI USA. GEORGE WASHINGTON UNIV HOSP, WASHINGTON, DC 20052 USA. COOK CTY HOSP, CHICAGO, IL USA. PITTSBURGH MERCY HOSP, PITTSBURGH, PA USA. MED COLL PENN, PHILADELPHIA, PA USA. MED UNIV S CAROLINA, CHARLESTON, SC USA. UNIV HOSP ALBUQUERQUE, ALBUQUERQUE, NM USA. GRADY MEM HOSP, ATLANTA, GA USA. STANFORD UNIV, STANFORD, CA USA. ST PAUL RAMSEY HOSP, ST PAUL, MN USA. UNIV CHICAGO HOSP, CHICAGO, IL USA. ROSE MED CTR, DENVER, CO USA. BETH ISRAEL MED CTR, NEW YORK, NY USA. UNIV TEXAS, SCH MED, AUSTIN, TX USA. UNIV WASHINGTON, SCH MED, SEATTLE, WA USA. EMORY UNIV HOSP, ATLANTA, GA USA. FAIRFAX HOSP, FALLS CHURCH, VA USA. UNIV CALIF IRVINE, IRVINE, CA USA. ST LUKES EPISCOPAL HOSP, HOUSTON, TX USA. MAINE MED CTR, PORTLAND, ME USA. MARSHFIELD CLIN FDN MED RES & EDUC, MARSHFIELD, WI USA. VET ADM MED CTR, SALT LAKE CITY, UT USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX USA. THERAPEUT RES INST, SARASOTA, FL USA. HARTFORD HOSP, HARTFORD, CT USA. SONORA LAB SCI, MESA, AZ USA. GOOD SAMARITAN HOSP, PHOENIX, AZ USA. BROOKE ARMY MED CTR, FT SAM HOUSTON, TX USA. ARKANSAS CHILDRENS HOSP, LITTLE ROCK, AR USA. UNIV MARYLAND HOSP, BALTIMORE, MD USA. VET ADM MED CTR, PORTLAND, OR USA. VET ADM MED CTR, CLEVELAND, OH USA. SUNY HLTH SCI CTR, BROOKLYN, NY USA. TAMPA GEN HOSP, TAMPA, FL USA. ALLEGHENY GEN HOSP, PITTSBURGH, PA USA. ST MICHAELS HOSP, NEWARK, NJ USA. UNIV WISCONSIN HOSP, MADISON, WI USA. UNIV HOSP CINCINNATI, CINCINNATI, OH USA. MED CTR DELAWARE, NEWARK, DE USA. MICHIGAN STATE UNIV, E LANSING, MI USA. VET ADM MED CTR, BOISE, ID USA. VANDERBILT UNIV SCH MED, NASHVILLE, TN USA. AKRON INFECT DIS, AKRON, OH USA. W VIRGINIA UNIV HOSP, MORGANTOWN, WV USA. UNIV ALABAMA HOSP & CLIN, BIRMINGHAM, AL USA. UNIV MED & DENT NEW JERSEY, NEW BRUNSWICK, NJ USA. MED CTR HOSP VERMONT, BURLINGTON, VT USA. MED COLL PENN, PHILADELPHIA, PA USA. LATTER DAY ST HOSP, SALT LAKE CITY, UT USA. LAKESIDE VET ADM MED CTR, CHICAGO, IL USA. VET ADM MED CTR, DURHAM, NC 27705 USA. RP JONES, RN (reprint author), UNIV IOWA, COLL MED, DEPT PATHOL, DIV MED MICROBIOL, 5232 RCP, IOWA CITY, IA 52242 USA. NR 42 TC 110 Z9 113 U1 0 U2 1 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0732-8893 J9 DIAGN MICR INFEC DIS JI Diagn. Microbiol. Infect. Dis. PD FEB PY 1995 VL 21 IS 2 BP 85 EP 93 DI 10.1016/0732-8893(94)00147-O PG 9 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA QX796 UT WOS:A1995QX79600006 PM 7628198 ER PT J AU BISSETTE, G GRIFF, D CARNES, M GOODMAN, B LAVINE, M LEVANT, B AF BISSETTE, G GRIFF, D CARNES, M GOODMAN, B LAVINE, M LEVANT, B TI APPARENT SEASONAL RHYTHMS IN HYPOTHALAMIC NEUROPEPTIDES IN RATS WITHOUT PHOTOPERIOD CHANGES SO ENDOCRINOLOGY LA English DT Article ID FACTOR-LIKE IMMUNOREACTIVITY; MANTLED GROUND-SQUIRRELS; CIRCANNUAL RHYTHMS; AFFECTIVE-DISORDER; SUPRACHIASMATIC NUCLEUS; OLFACTORY BULBECTOMY; DEPRESSED-PATIENTS; PERIOD; LESIONS; RESPONSES AB An apparent seasonal or circannual rhythm in the hypothalamic content of CRF, TRH, neurotensin, and neuromedin N has been observed in 12 separate monthly coherts (n = 10@ or 130 total) adult, male Sprague-Dawley rats obtained at the same time each month from a single commercial supplier and held under constant (12:12) photoperiod conditions since birth. Both annual and 4-month (terannual) harmonics can be statistically discerned in these apparent rhythms, which exhibit cycles containing concentration changes up to 3-fold the lowest levels across the year (CRF up arrow 390%, TRH up arrow 173%, neurotensin up arrow 136%, and neuromedin N up arrow 150%). Hypothalamic somatostatin did not exhibit these statistically significant robust rhythms nor did any peptide in regions outside the hypothalamus. These data indicate that a mechanism allowing enhanced or diminished physiological availability of these regulatory neuropeptides at different times of the year may exist and may display distinct cycles even in the absence of normal photoperiod cues. Possible regulatory responses of pituitary receptor populations for these hypothalamic peptides must be considered. As certain of these neuropeptides also appear to be altered in the cerebrospinal fluid of patients with major depression or schizophrenia, similar hypothalamic cyclic changes may underly psychiatric symptoms with seasonal periodicity. C1 UNIV WISCONSIN, MIDDLETON VET ADM HOSP, DEPT MED, MADISON, WI 53705 USA. DUKE UNIV, INST STAT & DECIS SCI, DURHAM, NC 27705 USA. UNIV KANSAS, MED CTR, DEPT PHARMACOL TOXICOL & THERAPEUT, KANSAS CITY, KS 66160 USA. RP BISSETTE, G (reprint author), DUKE UNIV, MED CTR, DEPT PHARMACOL & PSYCHIAT, BOX 3859, DURHAM, NC 27710 USA. FU NIMH NIH HHS [MH-45975] NR 44 TC 18 Z9 18 U1 1 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 J9 ENDOCRINOLOGY JI Endocrinology PD FEB PY 1995 VL 136 IS 2 BP 622 EP 628 DI 10.1210/en.136.2.622 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QD586 UT WOS:A1995QD58600034 PM 7835296 ER PT J AU WALTON, NY RUBINSTEIN, BK TREIMAN, DM AF WALTON, NY RUBINSTEIN, BK TREIMAN, DM TI CARDIAC-HYPERTROPHY SECONDARY TO STATUS EPILEPTICUS IN THE RAT SO EPILEPSY RESEARCH LA English DT Article DE STATUS EPILEPTICUS; CARDIAC HYPERTROPHY; RATS ID EPILEPTOGENIC ACTIVITY; NEURAL DISCHARGE; DEATH; DYSFUNCTION; PILOCARPINE; EPILEPSY; SEIZURES; DIAZEPAM; LITHIUM AB Status epilepticus was induced in rats by sequential injections of lithium and pilocarpine. Seizure activity was aborted by a combination of MK-801 and diazepam, with status duration ranging from 3 to 180 min. When the hearts were examined 8-12 days later, rats that had experienced an episode of status epilepticus had significantly heavier hearts than did controls. The nature of the cardiac tissue changes was not examined, and deserves further study. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. RP WALTON, NY (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEUROL & RES SERV,BLDG 304,E3-112,LOS ANGELES,CA 90073, USA. NR 17 TC 4 Z9 4 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-1211 J9 EPILEPSY RES JI Epilepsy Res. PD FEB PY 1995 VL 20 IS 2 BP 121 EP 124 DI 10.1016/0920-1211(94)00074-7 PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA QH610 UT WOS:A1995QH61000004 PM 7750508 ER PT J AU RUTECKI, PA AF RUTECKI, PA TI NORADRENERGIC MODULATION OF EPILEPTIFORM ACTIVITY IN THE HIPPOCAMPUS SO EPILEPSY RESEARCH LA English DT Article DE NOREPINEPHRINE; CA3 SUBFIELD; AFTERHYPERPOLARIZATION; PAROXYSMAL DEPOLARIZING SHIFT (PDS); PICROTOXIN; ELEVATED EXTRACELLULAR POTASSIUM ID RAT HIPPOCAMPUS; NETWORK EXCITABILITY; PYRAMIDAL CELLS; HIGH POTASSIUM; NORADRENALINE; NEURONS; EPILEPSY; INVITRO; AFTERHYPERPOLARIZATION; INITIATION AB Norepinephrine has been proposed to have both pro- and anticonvulsant properties. In the CA3 region of rat hippocampal slices, we studied the effects of norepinephrine and selective adrenergic agonists and antagonists on spontaneously occurring epileptiform discharges produced by either picrotoxin, a convulsant that impairs GABA(A)-mediated inhibition, or by elevated extracellular potassium ([K+](0)). Bath application of 5 mu M norepinephrine (NE) increased the rate of discharges produced by 7.5 mM [K+](0) but not the rate of picrotoxin-induced discharges. At higher concentrations (greater than or equal to 10 mu M), NE slowed the rate of spontaneous epileptiform discharges produced by picrotoxin. Spontaneous discharges produced by either picrotoxin or 7.5 mM [K+](0) were slowed or stopped by alpha-adrenergic receptor activation, the alpha(1) receptor being most responsible for this slowing effect. The alpha(2) agonist clonidine had minimal effects on the discharge rate; however, the alpha(2) antagonists yohimbine and idazoxan slowed the rate. In contrast, beta receptor or adenylate cyclase activation increased the rate of spontaneous discharges. This acceleration in rate was accompanied by a decrease in the amplitude and duration of the afterhyperpolarization (AHP) that follows the intracellularly recorded paroxysmal depolarizing shift (PDS). These results confirm that beta-adrenergic receptor activation increases the rate of epileptiform discharges and suggest that the acceleration is a result of a decrease in the AHP duration and amplitude. Activation of alpha-adrenergic receptors slowed the rate of epileptiform discharges without an associated change in the AHP. The AHP that follows the PDS helps define the maximal rate of epileptiform discharges in the hippocampal slice and a decrease in the duration of the AHP may contribute to the transition from an interictal to ictal pattern of epileptiform activity. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,FRANCIS M FORSTER EPILEPSY CTR,MADISON,WI 53705. RP RUTECKI, PA (reprint author), UNIV WISCONSIN,DEPT NEUROL,2500 OVERLOOK TR,MADISON,WI 53705, USA. FU NINDS NIH HHS [R29-NS28580] NR 40 TC 34 Z9 35 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-1211 J9 EPILEPSY RES JI Epilepsy Res. PD FEB PY 1995 VL 20 IS 2 BP 125 EP 136 DI 10.1016/0920-1211(94)00078-B PG 12 WC Clinical Neurology SC Neurosciences & Neurology GA QH610 UT WOS:A1995QH61000005 PM 7750509 ER PT J AU KIRIKAE, F KIRIKAE, T QURESHI, N TAKAYAMA, K MORRISON, DC NAKANO, M AF KIRIKAE, F KIRIKAE, T QURESHI, N TAKAYAMA, K MORRISON, DC NAKANO, M TI CD14 IS NOT INVOLVED IN RHODOBACTER-SPHAEROIDES DIPHOSPHORYL LIPID A INHIBITION OF TUMOR-NECROSIS-FACTOR-ALPHA AND NITRIC-OXIDE INDUCTION BY TAXOL IN MURINE MACROPHAGES SO INFECTION AND IMMUNITY LA English DT Article ID LIPOPOLYSACCHARIDE-BINDING PROTEINS; RHODOPSEUDOMONAS-SPHAEROIDES; LPS BINDING; BACTERIAL LIPOPOLYSACCHARIDE; NONTOXIC LIPOPOLYSACCHARIDE; BEARING PARTICLES; SOLUBLE CD14; FATTY-ACIDS; ENDOTOXIN; RECEPTORS AB Taxol, a microtubule stabilizer with anticancer activity, mimics the actions of lipopolysaccharide (LPS) on murine macrophages in vitro. Recently, it was shown that taxol-induced macrophage activation was inhibited by the LPS antagonist Rhodobacter sphaeroides diphosphoryl lipid A (RsDPLA). To investigate the mechanisms of taxol-induced macrophage activation, the present study focused on the interaction of LPS, RsDPLA, and taxol in the activation of and binding to macrophages. Taxol alone induced murine C3H/He macrophages to secrete tumor necrosis factor alpha (TNF) and to produce nitric oxide (NO) with kinetics similar to that of LPS. Macrophages from LPS-hyporesponsive C3H/HeJ mice, in contrast, did not yield any detectable TNF and NO production in response to LPS or taxol. RsDPLA inhibited taxol-induced TNF and NO production from C3H/He macrophages in a dose-dependent manner. The inhibition by RsDPLA was specific for LPS and taxol in that RsDPLA did not inhibit heat-killed Listeria monocytogenes- or zymosan-induced TNF production. Polymyxin B blocked the inhibitory effect of RsDPLA on taxol-induced TNF production. The inhibitory activity of RsDPLA appeared to be reversible since macrophages still responded to taxol in inducing TNF production after the RsDPLA was washed out with phosphate-buffered saline prior to the addition of taxol. Taxol-induced TNF production was not inhibited by colchicine, vinblastine, or 10-deacetylbaccatine III. A mutant cell line, J7.DEF3, defective in expression of a CD14 antigen, responded equally well to taxol by producing TNF as did the parent J774.1 cells. This suggested that the activation of macrophages by taxol does not require CD14. Taxol-induced TNF production by the mutant cells was also inhibited by RsDPLA. I-125-labeled LPS and H-3-labeled taxol was reported to bind to J774.1 cells predominantly via CD14 and microtubules, respectively. The binding of I-125-labeled LPS to J7.DEF3 cells was about 30 to 40% of that to J774.1 cells, The binding of I-125-LPS to J774.1 cells was inhibited by unlabeled LPS and RsDPLA but not by taxol. On the other hand, H-3-labeled taxol bound to both J774.1 cells and J7.DEF3 cells in similar time- and dose-dependent manners, The binding of [H-3] taxol to these cells was inhibited by taxol but not by LPS or RsDPLA. Although the binding studies failed to examine cross competition for binding to macrophages, a possible explanation of these results is that LPS, RsDPLA, and taxol share the same molecule(s) on murine macrophages for their functional receptor(s), which is neither CD14 nor tubulin. C1 JICHI MED SCH,DEPT MICROBIOL,MINAMI KAWACHI,TOCHIGI 32904,JAPAN. UNIV WISCONSIN,COLL AGR & LIFE SCI,DEPT BACTERIOL,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MYCOBACTERIOL RES LAB,MADISON,WI 53706. UNIV KANSAS,MED CTR,CTR CANC,KANSAS CITY,KS 66160. FU NCI NIH HHS [P01 CA54474]; NIAID NIH HHS [R37 AI23447]; NIGMS NIH HHS [GM36054] NR 59 TC 52 Z9 52 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1995 VL 63 IS 2 BP 486 EP 497 PG 12 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA QC603 UT WOS:A1995QC60300017 PM 7529746 ER PT J AU MAGEE, DM WILLIAMS, DM SMITH, JG BLEICKER, CA GRUBBS, BG SCHACHTER, J RANK, RG AF MAGEE, DM WILLIAMS, DM SMITH, JG BLEICKER, CA GRUBBS, BG SCHACHTER, J RANK, RG TI ROLE OF CD8 T-CELLS IN PRIMARY CHLAMYDIA INFECTION SO INFECTION AND IMMUNITY LA English DT Article ID MOUSE PNEUMONITIS AGENT; NECROSIS-FACTOR-ALPHA; IFN-GAMMA; BETA-2-MICROGLOBULIN-DEFICIENT MICE; LISTERIA-MONOCYTOGENES; DEFICIENT MICE; HOST DEFENSE; CRYPTOCOCCUS-NEOFORMANS; TRACHOMATIS INFECTION; TOXOPLASMA-GONDII AB The role of CD4 and CD8 T cells in primary Chlamydia trachomatis pneumonia was investigated by using in vivo depletion techniques to eliminate T cell populations. Reduction of either CD4 T cells or CD8 T cells caused a significant increase in organism burden in the lungs, as measured by both quantitative culture and detection of chlamydial antigen on day 14 postinfection. Chlamydia-specific antibody levels in plasma or antigen-induced gamma interferon (IFN-gamma) production by spleen cells was dramatically reduced by depletion of CD4 cells. The reduction in IFN-gamma achieved by depletion of CD8 cells did not reach statistical significance. In the survival studies, depletion of CD4 cells led to a significant increase in mortality. Although there was a trend toward higher mortality, depletion of CD8 cells did not significantly increase mortality. The role of CD8 T cells in host defense was clarified in studies using beta 2-microglobulin-deficient (major histocompatibility class I antigen-deficient, C1D) mice which are defective in CD8 T-cell function. In this model, a significant increase in organism burden was seen during infection in C1D mice compared with that in C57BL/6 controls and a significant increase in mortality was observed as well. However, surviving C1D mice were able to clear the infection by day 34. C1D mice had increased numbers of CD4 T cells in both the spleen and the lungs during infection compared,vith those of C57BL/6 controls. IFN-gamma in C57BL/6 mice was produced by both CD4 and CD8 cells. Thus, there is a protective role for both CD4 and CD8 cells in host defense against Chlamydia infection, but the former appear to be dominant. C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,DIV INFECT DIS,SAN ANTONIO,TX 78284. UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94132. UNIV ARKANSAS MED SCI HOSP,DEPT MICROBIOL & IMMUNOL,LITTLE ROCK,AR 72205. RP MAGEE, DM (reprint author), TEXAS CTR INFECT DIS,DEPT IMMUNOL RES,SAN ANTONIO,TX 78223, USA. FU NIAID NIH HHS [AI 22380, AI 26328] NR 46 TC 76 Z9 78 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD FEB PY 1995 VL 63 IS 2 BP 516 EP 521 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA QC603 UT WOS:A1995QC60300021 PM 7822016 ER PT J AU FLEMING, K GREEN, MF AF FLEMING, K GREEN, MF TI BACKWARD-MASKING PERFORMANCE DURING AND AFTER MANIC EPISODES SO JOURNAL OF ABNORMAL PSYCHOLOGY LA English DT Article ID SCHIZOPHRENIA SPECTRUM DISORDERS AB In a longitudinal design, 16 inpatients with bipolar mood disorder and 16 normal control participants were administered measures of backward masking. Bipolar inpatients were assessed while actively manic and again following manic episode. Clinical state was determined from ratings on an expanded version of the Brief Psychiatric Rating Scale. Two backward masking paradigms were used: (a) a staircase method, which yielded a critical interstimulus interval, and (b) set interstimulus intervals, which provided a masking function. Bipolar patients performed significantly worse than the normal controls at both sessions, but the Group X Session interaction was nonsignificant with both masking procedures. The masking performance deficit for the manic patients was not related to the presence of psychotic symptoms but seemed to be partially associated with lithium treatment. The results indicate that the impaired masking performance of manic patients is not strictly limited to the period of the manic episode. C1 UNIV CALIF LOS ANGELES, RES CTR, DEPT PSYCHIAT & BIOBEHAV SCI, POB 6022, CAMARILLO, CA 93011 USA. W LOS ANGELES VET AFFAIRS MED CTR, LOS ANGELES, CA USA. ST ELIZABETH HOSP, WASHINGTON, DC 20032 USA. NIMH, BETHESDA, MD 20892 USA. FU NIMH NIH HHS [MH-30911, MH-43292] NR 32 TC 26 Z9 26 U1 0 U2 2 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 USA SN 0021-843X J9 J ABNORM PSYCHOL JI J. Abnorm. Psychol. PD FEB PY 1995 VL 104 IS 1 BP 63 EP 68 PG 6 WC Psychology, Clinical; Psychology, Multidisciplinary SC Psychology GA QD428 UT WOS:A1995QD42800007 PM 7897054 ER PT J AU OGAWA, N DANG, H TALAL, N AF OGAWA, N DANG, H TALAL, N TI APOPTOSIS AND AUTOIMMUNITY SO JOURNAL OF AUTOIMMUNITY LA English DT Review ID TRANSFORMING GROWTH FACTOR-BETA-1; PROGRAMMED CELL-DEATH; ACTIVATED HUMAN-LYMPHOCYTES; FAS ANTIGEN; T-CELLS; LPR MICE; B-CELLS; DNA FRAGMENTATION; BCL-2 EXPRESSION; CROSS-LINKING C1 UNIV TEXAS,HLTH SCI CTR,DEPT MED,DIV CLIN IMMUNOL,SAN ANTONIO,TX 78284. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. FU NIDCR NIH HHS [DE 10863, DE 09311] NR 109 TC 34 Z9 36 U1 0 U2 0 PU ACADEMIC PRESS (LONDON) LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0896-8411 J9 J AUTOIMMUN JI J. Autoimmun. PD FEB PY 1995 VL 8 IS 1 BP 1 EP 19 DI 10.1006/jaut.1995.0001 PG 19 WC Immunology SC Immunology GA QF809 UT WOS:A1995QF80900001 PM 7734031 ER PT J AU UY, HL DALLAS, M CALLAND, JW BOYCE, BF MUNDY, GR ROODMAN, GD AF UY, HL DALLAS, M CALLAND, JW BOYCE, BF MUNDY, GR ROODMAN, GD TI USE OF AN IN-VIVO MODEL TO DETERMINE THE EFFECTS OF INTERLEUKIN-1 ON CELLS AT DIFFERENT STAGES IN THE OSTEOCLAST LINEAGE SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article ID COLONY-STIMULATING FACTOR; BONE-RESORPTION INVITRO; HUMAN MARROW CULTURES; PAGETS-DISEASE; PROGENITOR CELLS; STROMAL CELLS; FACTOR-ALPHA; NORMAL MICE; INVIVO; BISPHOSPHONATES AB In vitro model systems have been used extensively to study factors that affect osteoclast formation and to identify osteoclast precursors. However, in vitro systems do not examine the entire process of osteoclast differentiation simultaneously and lack accessory cells normally present in vivo. Additionally, the role that metabolism of the factor may play on its osteotropic activity in vivo is not addressed by these culture systems. Therefore, we have developed an in vivo model that permits us to examine simultaneously the effects of osteotropic factors on three distinct stages of osteoclast differentiation: (1) multipotent osteoclast precursors, the granulocyte-macrophage colony-forming unit (CFU-GM); (2) more differentiated marrow mononuclear osteoclast precursors; and (3) mature osteoclasts already present on bone surfaces. In the current study, we used interleukin-1 (IL-1) as a prototypic osteotropic factor to test the utility of this system to delineate the cellular mechanisms responsible for enhanced osteoclast activity stimulated by this cytokine. IL-1 induced hypercalcemia and enhanced the growth and differentiation of CFU-GM, increased the number of more committed mononuclear osteoclast precursors, and stimulated mature osteoclasts to resorb bone. These data demonstrate that this simple in vivo model permits the easy delineation of the stages of osteoclast development, in which osteotropic factors act to enhance bone turnover, and may be useful in understanding the mechanism of action of antiresorptive agents. C1 AUDIE L MURPHY MEM VET ADM MED CTR,RES SERV 151,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT ENDODONT,SAN ANTONIO,TX. UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,SAN ANTONIO,TX 78284. FU NIADDK NIH HHS [AM 35188]; NIAMS NIH HHS [AR 41336, AR 39539] NR 29 TC 42 Z9 44 U1 0 U2 0 PU BLACKWELL SCIENCE PUBL INC CAMBRIDGE PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD FEB PY 1995 VL 10 IS 2 BP 295 EP 301 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QF460 UT WOS:A1995QF46000016 PM 7754810 ER PT J AU LARUE, A OHARA, R MATSUYAMA, SS JARVIK, LF AF LARUE, A OHARA, R MATSUYAMA, SS JARVIK, LF TI COGNITIVE CHANGES IN YOUNG-OLD ADULTS - EFFECT OF FAMILY HISTORY OF DEMENTIA SO JOURNAL OF CLINICAL AND EXPERIMENTAL NEUROPSYCHOLOGY LA English DT Article ID ALZHEIMERS-DISEASE; AGE; PERFORMANCE; HEALTH; RISK AB Cognitive performance of 40 first-degree relatives of patients with probable Alzheimer disease was compared to that of 24 matched controls without a family history of dementia. Across a test-retest interval ranging from 1 to 6 years, relatives more often showed evidence of cognitive decline, and in multivariate analyses of memory and intelligence measures, relatives of patients with early-onset dementia (< 67 years) showed greater decline than controls or relatives of patients with late-onset dementia. All changes observed to date are in the subclinical range, and further follow-up will be needed to determine the reliability of change trajectories. However, the findings suggest that family history of dementia may be worthy of monitoring in research on normal cognitive aging. C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,LOS ANGELES,CA. FU NIMH NIH HHS [MH36205] NR 21 TC 23 Z9 23 U1 0 U2 1 PU SWETS ZEITLINGER BV PI LISSE PA P O BOX 825, 2160 SZ LISSE, NETHERLANDS SN 1380-3395 J9 J CLIN EXP NEUROPSYC JI J. Clin. Exp. Neuropsychol. PD FEB PY 1995 VL 17 IS 1 BP 65 EP 70 DI 10.1080/13803399508406582 PG 6 WC Psychology, Clinical; Clinical Neurology; Psychology SC Psychology; Neurosciences & Neurology GA QJ150 UT WOS:A1995QJ15000008 PM 7608303 ER PT J AU REILLY, PM SEES, KL SHOPSHIRE, MS HALL, SM DELUCCHI, KL TUSEL, DJ BANYS, P CLARK, HW PIOTROWSKI, NA AF REILLY, PM SEES, KL SHOPSHIRE, MS HALL, SM DELUCCHI, KL TUSEL, DJ BANYS, P CLARK, HW PIOTROWSKI, NA TI SELF-EFFICACY AND ILLICIT OPIOID USE IN A 180-DAY METHADONE DETOXIFICATION TREATMENT SO JOURNAL OF CONSULTING AND CLINICAL PSYCHOLOGY LA English DT Article ID DRUG AB Self-efficacy ratings coincided with illicit opioid use across the 3 phases of a 180-day methadone detoxification treatment. Efficacy ratings increased after patients received their first dose of methadone, did not change while they were maintained on a stable dose of methadone, and declined during the taper as they attempted to face high-risk situations without the full benefit of methadone. Efficacy ratings measured at a point before a phase of treatment predicted illicit opioid use across that phase. For clarification of the relation between self-efficacy and illicit opioid use, 3 conceptual models proposed by J.S. Baer, C. S. Holt, and E. Lichtenstein (1986) were tested. Self-efficacy influenced subsequent drug use in parallel with previous behavior, but this influence was found only at the start of the stabilization phase and immediately before the start of the taper phase. These findings highlight the usefulness of the self-efficacy concept for the treatment of opioid addiction. C1 UNIV CALIF SAN FRANCISCO,DEPT PSYCHIAT,SAN FRANCISCO,CA 94143. RP REILLY, PM (reprint author), SAN FRANCISCO VET AFFAIRS MED CTR,SUBSTANCE ABUSE PROGRAMS,116-E,4150 CLEMENT ST,SAN FRANCISCO,CA 94121, USA. FU NIDA NIH HHS [1R18DA06097] NR 8 TC 33 Z9 33 U1 0 U2 1 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 SN 0022-006X J9 J CONSULT CLIN PSYCH JI J. Consult. Clin. Psychol. PD FEB PY 1995 VL 63 IS 1 BP 158 EP 162 PG 5 WC Psychology, Clinical SC Psychology GA QG936 UT WOS:A1995QG93600022 PM 7896984 ER PT J AU ROOK, AH KUBIN, M CASSIN, M VONDERHEID, EC VOWELS, BR WOLFE, JT WOLF, SF SINGH, A TRINCHIERI, G LESSIN, SR AF ROOK, AH KUBIN, M CASSIN, M VONDERHEID, EC VOWELS, BR WOLFE, JT WOLF, SF SINGH, A TRINCHIERI, G LESSIN, SR TI IL-12 REVERSES CYTOKINE AND IMMUNE ABNORMALITIES IN SEZARY-SYNDROME SO JOURNAL OF IMMUNOLOGY LA English DT Article ID CELL STIMULATORY FACTOR; INTERFERON-GAMMA-PRODUCTION; MYCOSIS-FUNGOIDES; T-CELL; PERIPHERAL-BLOOD; INTERLEUKIN-12; SECRETION; LYMPHOMA; LYMPHOCYTES; PATTERN AB Cutaneous T cell lymphoma is a lymphoproliferative disorder typically characterized by skin invasion of clonally derived malignant CD4(+) lymphocytes that phenotypically resemble mature Th cells. Sezary syndrome (SzS) represents an advanced form of cutaneous T cell lymphoma associated with generalized erythroderma and involvement of the peripheral blood by the malignant cell population. We have previously demonstrated aberrant cytokine production by PBMC in SzS characterized by increased IL-4 and deficient IL-2 and IFN-gamma production, as well as increased expression of mRNA for IL-4 and IL-5 within active skin lesions, suggesting that the clonal T cell population is derived from the Th 2 subset of helper T lymphocytes. These findings have been associated with a constellation of immune abnormalities that have been attributed to the cytokine abnormalities. Because IL-12 is a potent inducer of IFN-gamma production, and causes the activation of cytotoxic lymphocytes, we examined the production of IL-12 by PBMC from SzS patients and whether IL-12 could alter the unfavorable cytokine balance typical of SzS and thus lead to correction of immune defects. Despite normal numbers of peripheral blood monocytes and normal TNF-alpha production, mean Staphyloccus aureus and LPS-induced IL-12 p40 and p70 production by SzS PBMC was significantly decreased compared with PBMC from normal controls. Mean IFN-gamma production by patient PBMC in response to PHA alone was depressed, but increased to levels comparable with normal after addition of 1 ng/ml IL-12. Pretreatment of PBMC for 24 h with IL-12, IFN-alpha, or both together resulted in a decrease in PHA-stimulated IL-4 production from a base line of 1818 pg/ml to 1520, 1350, and 1058 pg/ml, respectively. Lastly, culture of patient PBMC with IL-l 2 for 24 h also resulted in significant increases in NK activity against K562 cells. These results indicate that PBMC from patients with SzS manifest a defect in IL-12 production and that the cytokine abnormalities associated with SzS can be favorably altered by IL-12. C1 WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104. HAHNEMANN UNIV,PHILADELPHIA,PA 19104. DEPT VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. GENET INST INC,CAMBRIDGE,MA 02140. RP ROOK, AH (reprint author), UNIV PENN,MED CTR,DEPT DERMATOL,3400 SPRUCE ST,PHILADELPHIA,PA 19104, USA. FU NCI NIH HHS [1RO1 CA58841, R29 CA55017] NR 26 TC 107 Z9 107 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD FEB 1 PY 1995 VL 154 IS 3 BP 1491 EP 1498 PG 8 WC Immunology SC Immunology GA QC546 UT WOS:A1995QC54600055 PM 7822812 ER PT J AU XIA, EN RAO, G VANREMMEN, H HEYDARI, AR RICHARDSON, A AF XIA, EN RAO, G VANREMMEN, H HEYDARI, AR RICHARDSON, A TI ACTIVITIES OF ANTIOXIDANT ENZYMES IN VARIOUS TISSUES OF MALE FISCHER-344 RATS ARE ALTERED BY FOOD RESTRICTIONS SO JOURNAL OF NUTRITION LA English DT Article DE GLUTATHIONE PEROXIDASE; FOOD RESTRICTION; SUPEROXIDE DISMUTASE; CATALASE; RATS ID DIETARY RESTRICTION; SUPEROXIDE-DISMUTASE; DROSOPHILA-MELANOGASTER; BIOCHEMICAL PARAMETERS; LIPID-PEROXIDATION; OXIDATIVE STRESS; WISTAR RATS; LIFE-SPAN; AGE; EXPRESSION AB The objective of this study was to determine how food restriction (40% restriction of food intake) altered the age-related changes in the activities of Cu,Zn superoxide dismutase, catalase and glutathione peroxidase in liver, brain cortex, heart, kidney and intestinal mucosa obtained from 6-, 16- and 26-mo-old male Fischer 344 rats. Food restriction increased the activity of one or more of the antioxidant enzymes in the liver, brain cortex, heart and kidney of the rats. However, the magnitude of the effect and the antioxidant enzyme(s) affected by food restriction varied from tissue to tissue, and food restriction had no significant effect on the activities of these enzymes in intestinal mucosa. Interestingly, the four tissues in which food restriction increased the activity of one or more of the antioxidant enzymes showed reduced lipid peroxidation as measured by thiobarbituric acid-reactive material. These data suggest that food restriction might enhance the survival of rodents by altering the levels of the antioxidant enzymes and hence reducing free radical damage. C1 AUDIE L MURPHY MEM VET ADM MED CTR,EDUC & CLIN CTR,SAN ANTONIO,TX 78284. UNIV TEXAS,HLTH SCI CTR,DEPT MED,SAN ANTONIO,TX 78284. FU NIA NIH HHS [AG01548] NR 41 TC 110 Z9 114 U1 2 U2 5 PU AMER INST NUTRITION PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3166 J9 J NUTR JI J. Nutr. PD FEB PY 1995 VL 125 IS 2 BP 195 EP 201 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA QE851 UT WOS:A1995QE85100006 PM 7861246 ER PT J AU BUCKNER, CK FISHLEDER, RI CONKLIN, R GRAZIANO, FM AF BUCKNER, CK FISHLEDER, RI CONKLIN, R GRAZIANO, FM TI A COMPARISON OF THE EFFECTS OF ISOPROTERENOL AND FORSKOLIN ON IMMUNOLOGICAL AND NONIMMUNOLOGICAL RELEASE OF HISTAMINE FROM GUINEA-PIG SUPERFUSED TRACHEA AND DISPERSED TRACHEAL CELLS SO JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS LA English DT Article DE GUINEA-PIG TRACHEA; HISTAMINE RELEASE; ISOPROTERENOL; FORSKOLIN; ANTIGEN; CURARE ID INDUCED CONTRACTION; EVOKE CONTRACTION; MEDIATOR RELEASE; LUNG; INHIBITION; OVALBUMIN; ABILITY; STRIPS AB This study has compared the abilities of isoproterenol and forskolin to inhibit immunologic- and nonimmunologic-induced histamine release from guinea-pig superfused trachea and enzymatically dispersed tracheal cells. Contraction was also measured in the former preparation. The potency of isoproterenol was similar for inhibition of all parameters associated with immunologic (ovalbumin) challenge in the two preparations. In contrast, forskolin appeared less potent in inhibiting ovalbumin-induced histamine release from dispersed tracheal cells. Histamine release by the nonimmunologic secretagogues d-tubocurarine and compound 48/80 was not altered by either substance. However, inhibition by isoproterenol and forskolin of tracheal contraction was evident when challenge was conducted with d-tubocurarine and compound 48/80. Inhibition of contraction appears to be a result of functional antagonism at the level of the smooth muscle. The superfused trachea is a useful preparation in which to explore the effects of substances that modulate mast cell mediator release. C1 SCH PHARM,MADISON,WI. DEPT ANESTHESIOL,MADISON,WI. DEPT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. FU NHLBI NIH HHS [HL 28585] NR 14 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL CO INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 1056-8719 J9 J PHARMACOL TOXICOL JI J. Pharmacol. Toxicol. Methods PD FEB PY 1995 VL 33 IS 1 BP 47 EP 52 DI 10.1016/1056-8719(94)00056-A PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA QE859 UT WOS:A1995QE85900007 PM 7537127 ER PT J AU SCHUMACHER, HR AF SCHUMACHER, HR TI SYNOVIAL INFLAMMATION, CRYSTALS, AND OSTEOARTHRITIS SO JOURNAL OF RHEUMATOLOGY LA English DT Article; Proceedings Paper CT 3rd International Symposium on Osteoarthritis - Challenges for the 21st-Century CY OCT 04-07, 1994 CL VAL DAVID, CANADA DE OSTEOARTHRITIS; SYNOVIUM; SYNOVIAL FLUID; CPPD CRYSTALS; APATITE; INFLAMMATION ID CALCIUM PYROPHOSPHATE DIHYDRATE; ALIZARIN RED; OSTEO-ARTHRITIS; FLUID; KNEE; JOINT; HYDROXYAPATITE; DEPOSITION; MICROSCOPY; ACETAMINOPHEN AB Inflammation is now generally accepted as a component in the course of most patients with symptomatic osteoarthritis (OA), although the stage at which it occurs and how it affects the course has not been resolved. When joint effusions are present in OA they almost always contain more leukocytes than normal synovial fluid, although often still falling into what was considered a noninflammatory range. Multiple factors can contribute to this low grade inflammation, with crystals being only one of many such factors needing consideration. Crystals of calcium pyrophosphate dihydrate (CPPD) and apatite are more frequent in more severe radiographic OA, but whether these are a result, a contributing factor, or both is not known. Experimental studies and the few clinical observations do not show that either CPPD or apatite consistently cause either inflammation or more rapid destruction in OA. These attempts at correlations are plagued with technical questions about what crystal numbers may be meaningful, and about techniques for crystal identification. Continued efforts are indicated to identify a treatable component of OA. C1 UNIV PENN,SCH MED,DEPT MED,DIV RHEUMATOL,PHILADELPHIA,PA 19104. DEPT VET AFFAIRS MED CTR,CTR ARTHRITIS IMMUNOL,PHILADELPHIA,PA 19104. NR 33 TC 14 Z9 14 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD FEB PY 1995 VL 22 SU 43 BP 101 EP 103 PG 3 WC Rheumatology SC Rheumatology GA QH907 UT WOS:A1995QH90700032 ER PT J AU CRESS, ME SCHECHTMAN, KB MULROW, CD FIATARONE, MA GERETY, MB BUCHNER, DM AF CRESS, ME SCHECHTMAN, KB MULROW, CD FIATARONE, MA GERETY, MB BUCHNER, DM TI RELATIONSHIP BETWEEN PHYSICAL PERFORMANCE AND SELF-PERCEIVED PHYSICAL FUNCTION SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID HEALTH-STATUS; FICSIT TRIALS; VALIDITY; SCALES; AIMS AB OBJECTIVE: The objective of this study was to compare two methods of measuring physical function in subjects with a broad range of abilities and to evaluate the effects of cognitive, social, educational, and age factors on the relationship between the two methods. DESIGN: Multiple regression analysis was used to compare self-perceived (dependent variables) with performance measures (independent variables). Covariates included age, gender, Mini-Mental State Exam score, education, living status, and depression score. SETTING: Five community-dwelling and two nursing home sites. PARTICIPANTS: 417 community-dwelling subjects and 200 nursing home residents aged 62-98 years. MEASUREMENTS: Self-perceived physical function was assessed with the physical dimension summary score of the Sickness Impact Profile, which comprises three subscales: ambulation, mobility, and body care and movement. Physical performance was evaluated by self-selected gait speed, chair-stand time, maximal grip strength, and a balance score. RESULTS: Nursing home residents and community-dwellers were significantly different (P < .0001) in all variables except age and gender. Self-perceived and performance-based measures were moderately correlated, with a range from r = -.194 to r = -.625 (P < .05). Gait speed was the strongest independent predictor of self-perceived physical function in both groups. Symptoms of depression were also an independent predictor of self-perceived function in nursing home residents; subjects who had such symptoms report more self-perceived dysfunction than would be predicted based on performance tests. CONCLUSIONS: Self-selected gait speed is a global indicator of self-perceived physical function over a broad range of abilities. External determinants (depressive symptoms, cognitive function, marital status, etc.) affect self-perceived function in both groups, but gait speed is the greatest single predictor of self-perceived function. In nursing home residents depressive symptomatology is related to self-perceived C1 WASHINGTON UNIV,ST LOUIS,MO. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. HARVARD UNIV,SCH MED,BOSTON,MA. TUFTS UNIV,USDA,HUMAN NUTR RES CTR AGING,BOSTON,MA 02111. RP CRESS, ME (reprint author), UNIV WASHINGTON,HARBORVIEW MED CTR ZA87,325 9TH AVE,SEATTLE,WA 98104, USA. FU NIA NIH HHS [U-01AG09089] NR 27 TC 169 Z9 172 U1 3 U2 15 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD FEB PY 1995 VL 43 IS 2 BP 93 EP 101 PG 9 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QF230 UT WOS:A1995QF23000001 PM 7836655 ER PT J AU WALD, TG MILLER, BA SHULT, P DRINKA, P LANGER, L GRAVENSTEIN, S AF WALD, TG MILLER, BA SHULT, P DRINKA, P LANGER, L GRAVENSTEIN, S TI CAN RESPIRATORY SYNCYTIAL VIRUS AND INFLUENZA-A BE DISTINGUISHED CLINICALLY IN INSTITUTIONALIZED OLDER PERSONS SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Note ID NURSING-HOME; INFECTION; OUTBREAK; PNEUMONIA; ILLNESS; TRANSMISSION; ADULTS C1 UNIV WISCONSIN,INST AGING,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED,GERIATR & GERONTOL SECT,MADISON,WI. STATE LAB HYG,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,MADISON,WI 53705. WISCONSIN VET HOME,KING,WI 54946. RI Gravenstein, Stefan/G-1681-2011 FU NIA NIH HHS [5AG00213, AG09632, AG00548] NR 33 TC 41 Z9 41 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD FEB PY 1995 VL 43 IS 2 BP 170 EP 174 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA QF230 UT WOS:A1995QF23000013 PM 7836643 ER PT J AU STERLING, K BRENNER, MA AF STERLING, K BRENNER, MA TI THYROID-HORMONE ACTION - EFFECT OF TRIIODOTHYRONINE ON MITOCHONDRIAL ADENINE-NUCLEOTIDE TRANSLOCASE IN-VIVO AND IN-VITRO SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID RAT-LIVER MITOCHONDRIA; BINDING-SITES; GENE-EXPRESSION; RECEPTOR; LOCALIZATION; MEMBRANE; PATHWAY; STIMULATION; THYMOCYTES; KIDNEY AB Adenine nucleotide translocase (AdNT) levels were measured as the exchange of extramitochondrial against intramitochondrial adenosine diphosphate (ADP) in liver, spleen, and testes mitochondria isolated from normal and hypothyroid rats using the ''back-exchange'' and atractyloside-stop method of Pfaff and Klingenberg. The results provide confirmation of previous reports that mitochondria from hypothyroid rats show a markedly diminished AdNT activity, which is restored to normal levels within 72 hours by intraperitoneal injection of 10 to 20 mu g triiodothyronine (T-3)/100 g bodyweight. The latter dose was found in dose-response studies to result in maximal stimulation of AdNT in liver mitochondria. Qualitatively similar results on AdNT activity were obtained in liver mitochondria within 30 to 60 minutes following intravenous injection into hypothyroid rats of a more physiological dose of T-3 (40 ng/100 g body weight). AdNT in mitochondria isolated from spleen and testes (organs that do not exhibit a calorigenic response after administration of thyroid hormone to the whole animal) failed to respond to thyroidectomy and to administration of T-3. More recently, we have observed that in vitro replacement of T-3 also stimulates AdNT activity in hypothyroid liver mitochondria. The enzyme adenosine triphosphate (ATP) synthase was examined as another possible candidate for direct hormonal stimulation of mitochondria. Simultaneous determinations on the same rats after intraperitoneal injection of T-3 (20 mu g/100 g body weight) showed little or no effect on ATP synthase until after 37 to 85 hours, whereas enhanced activity of the translocator was regularly observed at 17 hours. These findings support the view that AdNT, which is considered to exert major control over the rate of oxidative phosphorylation, may be a direct target of Tg action on the mitochondria. Increased nuclear transcription may be regarded as a sustained delayed effect of T-3 administration, in contrast to the early effect an mitochondrial AdNT. A bolus intravenous injection of T-3 into the hypothyroid rat increases the activity of the mitochondrial carrier AdNT within a matter of minutes as an early direct effect, as also suggested by studies of addition of Tg in vitro to isolated rat liver mitochondria. In contrast, nuclear effects require 12 to 24 hours to show increased transcription, evidenced by increased specific mRNA directing the formation of more AdNT (Luciakova and Nelson). Copyright (C) 1995 by W.B. Saunders Company C1 BRONX VET ADM MED CTR,BRONX,NY. RP STERLING, K (reprint author), COLUMBIA UNIV,COLL PHYS & SURG,DEPT MED,630 W 168TH ST,NEW YORK,NY 10032, USA. FU NIDDK NIH HHS [DK 37870] NR 45 TC 29 Z9 30 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD FEB PY 1995 VL 44 IS 2 BP 193 EP 199 DI 10.1016/0026-0495(95)90264-3 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA QJ039 UT WOS:A1995QJ03900011 PM 7869915 ER PT J AU GOODFRIEND, TL ELLIOTT, ME AF GOODFRIEND, TL ELLIOTT, ME TI FATTY-ACIDS IN CELL SIGNALING - PREFACE SO PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS LA English DT Editorial Material C1 UNIV WISCONSIN,SCH MED,DEPT PHARMACOL,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,MADISON,WI. RP GOODFRIEND, TL (reprint author), UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH, MIDLOTHIAN, SCOTLAND EH1 3AF SN 0952-3278 J9 PROSTAG LEUKOTR ESS JI Prostaglandins Leukot. Essent. Fatty Acids PD FEB-MAR PY 1995 VL 52 IS 2-3 BP 75 EP 75 DI 10.1016/0952-3278(95)90000-4 PG 1 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism GA QJ382 UT WOS:A1995QJ38200001 ER EF