FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Babiker, EM Gordon, TC Chao, S Rouse, MN Wanyera, R Newcomb, M Brown-Guedira, G Pretorius, ZA Bonman, JM AF Babiker, E. M. Gordon, T. C. Chao, S. Rouse, M. N. Wanyera, R. Newcomb, M. Brown-Guedira, G. Pretorius, Z. A. Bonman, J. M. TI Genetic mapping of resistance to the Ug99 race group of Puccinia graminis f. sp tritici in a spring wheat landrace CItr 4311 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID STEM RUST RESISTANCE; AESTIVUM L.; 1ST REPORT; VIRULENCE; TTKS; IDENTIFICATION; PATHOGEN; AFRICA; LINES; SR31 AB A gene for Ug99 resistance from wheat landrace CItr 4311 was detected on the long arm of chromosome 2B. Wheat landrace CItr 4311 has seedling resistance to stem rust caused by Puccinia graminis f. sp. tritici race TTKSK and field resistance to the Ug99 race group. Parents, F-1 seedlings, 121 doubled haploid (DH) lines, and 124 recombinant inbred lines (RILs) developed from a cross between CItr 4311 and the susceptible line LMPG-6 were evaluated for seedling resistance to race TTKSK. Goodness-of-fit tests indicated that a single dominant gene in CItr 4311 conditioned the TTKSK resistance. The 90 K wheat iSelect SNP platform was used to genotype parents and the DH population. The seedling resistance locus was mapped to the chromosome arm 2BL. Parents and the DH population were evaluated for field resistance in Kenya. One major QTL for the field resistance was consistently detected in the same region on 2BL as the seedling resistance. Using KASP assays, five linked SNP markers were used to verify the result in the 124 RIL, 35 wheat accessions, 46 DH lines from the LMPG-6/PI 165194 cross and F-1 seedlings, and susceptible bulks derived from crosses between six resistant landraces with LMPG-6. Race specificity, mapping results, and haplotype similarity with lines with Sr9h (Gabo 56, Timstein, and PI 670015), support the hypothesis that the Sr gene in CItr 4311 and the landraces is Sr9h. The KASP assays developed in this study will be useful for pyramiding the TTKSK resistance from CItr 4311 with other Sr genes effective against Ug99. C1 [Babiker, E. M.; Gordon, T. C.; Bonman, J. M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. [Chao, S.] USDA ARS, Cereal Crops Res Unit, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Rouse, M. N.] USDA ARS, Cereal Dis Lab, 1551 Lindig Ave, St Paul, MN 55108 USA. [Wanyera, R.] Kenya Agr & Livestock Res Org, Njoro 20107, Kenya. [Newcomb, M.] Univ Arizona, Sch Plant Sci, Maricopa, AZ 85138 USA. [Brown-Guedira, G.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27606 USA. [Pretorius, Z. A.] Univ Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. RP Babiker, EM; Bonman, JM (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM Ebrahiem.babiker@ars.usda.gov; Mike.Bonman@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; USDA-ARS CRIS Project [2050-21000-029-00D]; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX We are very grateful to Sam Stoxen and Jayaveeramuthu Nirmala for their technical assistance. This research was supported by the USDA-ARS National Plant Disease Recovery System, the USDA-ARS CRIS Project 2050-21000-029-00D. This work was supported in part by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. NR 28 TC 0 Z9 0 U1 17 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD NOV PY 2016 VL 129 IS 11 BP 2161 EP 2170 DI 10.1007/s00122-016-2764-5 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EA4FJ UT WOS:000386565400010 PM 27544524 ER PT J AU Hiebert, CW Kassa, MT McCartney, CA You, FM Rouse, MN Fobert, P Fetch, TG AF Hiebert, Colin W. Kassa, Mulualem T. McCartney, Curt A. You, Frank M. Rouse, Matthew N. Fobert, Pierre Fetch, Tom G. TI Genetics and mapping of seedling resistance to Ug99 stem rust in winter wheat cultivar Triumph 64 and differentiation of SrTmp, SrCad, and Sr42 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; INFECTION TYPES; RACE TTKS; VIRULENCE; MAP; IDENTIFICATION; MARKERS; LINES AB Resistance to Ug99 stem rust in Triumph 64 was conferred by SrTmp on chromosome arm 6DS and was mapped to the same position as SrCad and Sr42 , however, the three genes show functional differences. Stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is an important disease of wheat that can be controlled by effective stem rust resistance (Sr) genes. The emergence of virulent Pgt races in Africa, namely Ug99 and its variants, has stimulated the search for new Sr genes and genetic characterization of known sources of resistance. Triumph 64 is a winter wheat cultivar that carries gene SrTmp, which confers resistance to Ug99. The goals of this study were to genetically map SrTmp and examine its relationship with other Sr genes occupying a similar chromosome location. A doubled haploid (DH) population from the cross LMPG-6S/Triumph 64 was inoculated with Ug99 at the seedling stage. A single gene conditioning resistance to Ug99 segregated in the population. Genetic mapping with SSR markers placed SrTmp on chromosome arm 6DS in a region similar to SrCad and Sr42. SNP markers developed for SrCad were used to further map SrTmp and were also added to a genetic map of Sr42 using a DH population (LMPG-6S/Norin 40). Three SNP markers that co-segregated with SrTmp also co-segregated with SrCad and Sr42. The SNP markers showed no difference in the map locations of SrTmp, SrCad, and Sr42. Multi-race testing with DH lines from the Triumph 64 and Norin 40 populations and a recombinant inbred-line population from the cross LMPG-6S/AC Cadillac showed that SrTmp, SrCad, and Sr42 confer different spectra of resistance. Markers closely linked to SrTmp are suitable for marker-assisted breeding and germplasm development. C1 [Hiebert, Colin W.; Kassa, Mulualem T.; McCartney, Curt A.; You, Frank M.] Agr & Agri Food Canada, Morden Res & Dev Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada. [Kassa, Mulualem T.; Fobert, Pierre] CNR, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada. [Rouse, Matthew N.] Univ Minnesota, USDA ARS, Cereal Dis Lab, Dept Plant Pathol, 1551 Lindig St, St Paul, MN 55108 USA. [Fetch, Tom G.] Agr & Agri Food Canada, Brandon Res & Dev Ctr, Brandon, MB R7A 5Y3, Canada. RP Hiebert, CW (reprint author), Agr & Agri Food Canada, Morden Res & Dev Ctr, 101 Route 100, Morden, MB R6M 1Y5, Canada. EM colin.hiebert@agr.gc.ca FU Agriculture and Agri-Food Canada Growing Forward; AAFC Agriflex project [2521]; AAFC A-base project [J-000006]; Canadian Wheat Alliance; CTAG and CTAG2, Genome Prairie projects - Genome Canada; Saskatchewan Ministry of Agriculture; Western Grain Research Foundation; USDA-ARS [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; Durable Rust Resistance in Wheat project FX The authors thank Taye Zegeye, Mira Popovic, Ghassan Mardli, Leslie Bezte, Tobi Malasiuk and Elaine Martineau for excellent technical assistance. We are grateful for the financial support from Agriculture and Agri-Food Canada Growing Forward, AAFC Agriflex project 2521, AAFC A-base project J-000006, the Canadian Wheat Alliance, and as part of CTAG and CTAG2, Genome Prairie projects funded by Genome Canada, Saskatchewan Ministry of Agriculture, and Western Grain Research Foundation. M. Rouse also acknowledges the support from USDA-ARS appropriated project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, and the Durable Rust Resistance in Wheat project. Based upon research contributions from the National Research Council of Canada, this publication was given the Contribution Number NRCC# 56224. NR 37 TC 2 Z9 2 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD NOV PY 2016 VL 129 IS 11 BP 2171 EP 2177 DI 10.1007/s00122-016-2765-4 PG 7 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EA4FJ UT WOS:000386565400011 PM 27506534 ER PT J AU Meinzer, FC Woodruff, DR Marias, DE Smith, DD McCulloh, KA Howard, AR Magedman, AL AF Meinzer, Frederick C. Woodruff, David R. Marias, Danielle E. Smith, Duncan D. McCulloh, Katherine A. Howard, Ava R. Magedman, Alicia L. TI Mapping "hydroscapes' along the iso- to anisohydric continuum of stomatal regulation of plant water status SO ECOLOGY LETTERS LA English DT Article DE Anisohydry; drought resistance; isohydry; leaf osmotic potential; leaf turgor; plant traits; plant water potential; stomata ID TURGOR LOSS POINT; PRESSURE-VOLUME RELATIONSHIPS; VINIFERA L. CULTIVARS; HYDRAULIC CONDUCTANCE; DROUGHT TOLERANCE; PSEUDOTSUGA-MENZIESII; EVAPORATIVE DEMAND; SEASONAL TRENDS; SUMMER DROUGHT; FOREST TREES AB The concept of iso- vs. anisohydry has been used to describe the stringency of stomatal regulation of plant water potential (). However, metrics that accurately and consistently quantify species' operating ranges along a continuum of iso- to anisohydry have been elusive. Additionally, most approaches to quantifying iso/anisohydry require labour-intensive measurements during prolonged drought. We evaluated new and previously developed metrics of stringency of stomatal regulation of during soil drying in eight woody species and determined whether easily-determined leaf pressure-volume traits could serve as proxies for their degree of iso- vs. anisohydry. Two metrics of stringency of stomatal control of , (1) a hydroscape' incorporating the landscape of over which stomata control , and (2) the slope of the daily range of as pre-dawn declined, were strongly correlated with each other and with the leaf osmotic potential at full and zero turgor derived from pressure-volume curves. C1 [Meinzer, Frederick C.; Woodruff, David R.] USDA Forest Serv, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Marias, Danielle E.; Magedman, Alicia L.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Smith, Duncan D.; McCulloh, Katherine A.] Univ Wisconsin, Dept Bot, Madison, WI 53705 USA. [Howard, Ava R.] Western Oregon Univ, Dept Biol, Monmouth, OR 97361 USA. RP Meinzer, FC (reprint author), USDA Forest Serv, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. EM Rick.Meinzer@oregonstate.edu FU NSF [IOS 11-46746] FX This work was supported by NSF grant IOS 11-46746. We are grateful to the OSU Greenhouse Operations staff members for their assistance with the maintenance of our plant material. NR 49 TC 0 Z9 0 U1 22 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD NOV PY 2016 VL 19 IS 11 BP 1343 EP 1352 DI 10.1111/ele.12670 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DZ9VL UT WOS:000386228300005 PM 27604411 ER PT J AU Dutta, T Kim, KH Uchimiya, M Kumar, P Das, S Bhattacharya, SS Szulejko, J AF Dutta, Tanushree Kim, Ki-Hyun Uchimiya, Minori Kumar, Pawan Das, Subhasish Bhattacharya, Satya Sundar Szulejko, Jan TI The micro-environmental impact of volatile organic compound emissions from large-scale assemblies of people in a confined space SO ENVIRONMENTAL RESEARCH LA English DT Review DE VOC; Exposure; Recreation; Sports; Football ID AIR-QUALITY; INDOOR AIR; CIGARETTE-SMOKE; ANTHROPOGENIC SOURCES; COMMONWEALTH GAMES; SKIN LIPIDS; VOCS; EXPOSURE; POLLUTION; OZONE AB Large-scale assemblies of people in a confined space can exert significant impacts on the local air chemistry due to human emissions of volatile organics. Variations of air-quality in such small scale can be studied by quantifying fingerprint volatile organic compounds (VOCs) such as acetone, toluene, and isoprene produced during concerts, movie screenings, and sport events (like the Olympics and the World Cup). This review summarizes the extent of VOC accumulation resulting from a large population in a confined area or in a small open area during sporting and other recreational activities. Apart from VOCs emitted directly from human bodies (e.g., perspiration and exhaled breath), those released indirectly from other related sources (e.g., smoking, waste disposal, discharge of food-waste, and use of personal-care products) are also discussed. Although direct and indirect emissions of VOCs from human may constitute <1% of the global atmospheric VOCs budget, unique spatiotemporal variations in VOCs species within a confined space can have unforeseen impacts on the local atmosphere to lead to acute human exposure to harmful pollutants. (C) 2016 Elsevier Inc All rights reserved. C1 [Dutta, Tanushree; Kim, Ki-Hyun; Szulejko, Jan] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. [Uchimiya, Minori] USDA ARS, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Kumar, Pawan] Indian Inst Technol, Dept Chem Engn, Hauz Khas, New Delhi 11016, India. [Das, Subhasish; Bhattacharya, Satya Sundar] Tezpur Univ, Dept Environm Sci, Soil & Agrobioengn Lab, Napaam 784028, India. RP Kim, KH (reprint author), Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. EM kkim61@hanyang.ac.kr RI KUMAR, PAWAN /E-5522-2012 OI KUMAR, PAWAN /0000-0003-0712-8763 FU National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) [2006-0093848]; Rural Development Administration, Republic of Korea [11010521] FX The corresponding author (KHK) acknowledges support from a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) (No. 2006-0093848) and by the Cooperative Research Program for Agriculture Science & Technology Development (Project title: Study on model development to control odor from hog barn, Project No. 11010521), Rural Development Administration, Republic of Korea. NR 87 TC 0 Z9 0 U1 19 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0013-9351 EI 1096-0953 J9 ENVIRON RES JI Environ. Res. PD NOV PY 2016 VL 151 BP 304 EP 312 DI 10.1016/j.envres.2016.08.009 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA EA2HO UT WOS:000386413600033 PM 27522568 ER PT J AU Ma, L Yates, SR Ashworth, D AF Ma, Li Yates, Scott R. Ashworth, Daniel TI PARENT AND CONJUGATED ESTROGENS AND PROGESTAGENS IN SURFACE WATER OF THE SANTA ANA RIVER: DETERMINATION, OCCURRENCE, AND RISK ASSESSMENT SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Hormones; Occurrence; Contamination sources; Risk assessment ID TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; ENDOCRINE DISRUPTING CHEMICALS; SEWAGE-TREATMENT WORKS; WASTE-WATER; STEROID-HORMONES; ACTIVATED-SLUDGE; TREATMENT PLANTS; DRINKING-WATER; PHARMACEUTICAL RESIDUES AB The present study investigated the occurrence of 13 parent and conjugated estrogens and progestagens in surface water of the Santa Ana River. With the exception of the synthetic hormones 17 alpha-ethynylestradiol and mestranol, other compounds were detected at least twice at 10 representative sites, with the ubiquitous estrone (E1) and 17 beta-estradiol-3-sulfate as the dominant compounds quantified (0.24-6.37 ng/L and 0.49-9.25 ng/L, respectively). Sites near dairy farms exhibited high levels of conjugates, whereas those close to a sewage treatment plant (STP) effluent outlet displayed relatively high concentrations of E1. Principle component analysis coupled with multiple linear regression revealed dairy farms and the STP as the 2 significant contamination sources, accounting for 69.9% and 31.1% of the total hormone burden, respectively. Risk assessment results suggested E1 and 17 beta-estradiol (E2) as the 2 hormones with the largest risks to aquatic organisms, and which combined, contributed >90% of the total estrogenicity. Most of the sites investigated showed that E1 and E2 posed a medium risk (0.1 < risk quotient < 1), whereas each induced a high risk (risk quotient > 1) at sites severely impacted by the STP and dairy farms. These results suggest that river health would benefit from effective treatment of waste at the STP and dairy farms prior to discharge. (C) 2016 SETAC C1 [Ma, Li; Ashworth, Daniel] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ma, Li; Yates, Scott R.; Ashworth, Daniel] ARS, Contaminant Fate & Transport Unit, Salin Lab, USDA, Riverside, CA 92503 USA. RP Ma, L (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA.; Ma, L (reprint author), ARS, Contaminant Fate & Transport Unit, Salin Lab, USDA, Riverside, CA 92503 USA. EM li.ma@ars.usda.gov NR 69 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD NOV PY 2016 VL 35 IS 11 BP 2657 EP 2664 DI 10.1002/etc.3447 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA EA2XU UT WOS:000386461000003 PM 27061433 ER PT J AU Cammarano, D Rotter, RP Asseng, S Ewert, F Wallach, D Martre, P Hatfield, JL Jones, JW Rosenzweig, C Ruane, AC Boote, KJ Thorburn, PJ Kersebaum, KC Aggarwal, PK Angulo, C Basso, B Bertuzzi, P Biernath, C Brisson, N Challinor, AJ Doltra, J Gayler, S Goldberg, R Heng, L Hooker, J Hunt, LA Ingwersen, J Izaurralde, RC Muller, C Kumar, SN Nendel, C O'Leary, GJ Olesen, JE Osborne, TM Palosuo, T Priesack, E Ripoche, D Semenov, MA Shcherbak, I Steduto, P Stockle, CO Stratonovitch, P Streck, T Supit, I Tao, F Travasso, M Waha, K White, JW Wolf, J AF Cammarano, Davide Rotter, Reimund P. Asseng, Senthold Ewert, Frank Wallach, Daniel Martre, Pierre Hatfield, Jerry L. Jones, James W. Rosenzweig, Cynthia Ruane, Alex C. Boote, Kenneth J. Thorburn, Peter J. Kersebaum, Kurt Christian Aggarwal, Pramod K. Angulo, Carlos Basso, Bruno Bertuzzi, Patrick Biernath, Christian Brisson, Nadine Challinor, Andrew J. Doltra, Jordi Gayler, Sebastian Goldberg, Richie Heng, Lee Hooker, Josh Hunt, Leslie A. Ingwersen, Joachim Izaurralde, Roberto C. Mueller, Christoph Kumar, Soora Naresh Nendel, Claas O'Leary, Garry J. Olesen, Jorgen E. Osborne, Tom M. Palosuo, Taru Priesack, Eckart Ripoche, Dominique Semenov, Mikhail A. Shcherbak, Iurii Steduto, Pasquale Stockle, Claudio O. Stratonovitch, Pierre Streck, Thilo Supit, Iwan Tao, Fulu Travasso, Maria Waha, Katharina White, Jeffrey W. Wolf, Joost TI Uncertainty of wheat water use: Simulated patterns and sensitivity to temperature and CO2 SO FIELD CROPS RESEARCH LA English DT Article DE Multi-model simulation; Transpiration efficiency; Water use; Uncertainty; Sensitivity ID CLIMATE-CHANGE; USE EFFICIENCY; CROP YIELD; POTENTIAL EVAPOTRANSPIRATION; ATMOSPHERIC CO2; FOOD SECURITY; ELEVATED CO2; IMPACTS; PRODUCTIVITY; MODELS AB Projected global warming and population growth will reduce future water availability for agriculture. Thus, it is essential to increase the efficiency in using water to ensure crop productivity. Quantifying crop water use (WU; i.e. actual evapotranspiration) is a critical step towards this goal. Here, sixteen wheat simulation models were used to quantify sources of model uncertainty and to estimate the relative changes and variability between models for simulated WU, water use efficiency (WUE, WU per unit of grain dry mass produced), transpiration efficiency (T-eff, transpiration per kg of unit of grain yield dry mass produced), grain yield, crop transpiration and soil evaporation at increased temperatures and elevated atmospheric carbon dioxide concentrations ([CO2]). The greatest uncertainty in simulating water use, potential evapotranspiration, crop transpiration and soil evaporation was due to differences in how crop transpiration was modelled and accounted for 50% of the total variability among models. The simulation results for the sensitivity to temperature indicated that crop WU will decline with increasing temperature due to reduced growing seasons. The uncertainties in simulated crop WU, and in particularly due to uncertainties in simulating crop transpiration, were greater under conditions of increased temperatures and with high temperatures in combination with elevated atmospheric [CO2] concentrations. Hence the simulation of crop WU, and in particularly crop transpiration under higher temperature, needs to be improved and evaluated with field measurements before models can be used to simulate climate change impacts on future crop water demand. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cammarano, Davide; Asseng, Senthold; Jones, James W.; Boote, Kenneth J.] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Rotter, Reimund P.; Palosuo, Taru; Tao, Fulu] Nat Resources Inst Finland Luke, FI-00790 Helsinki, Finland. [Ewert, Frank; Angulo, Carlos] Univ Bonn, Inst Crop Sci & Resource Conservat INRES, D-53115 Bonn, Germany. [Wallach, Daniel] INRA, Agrosyst & Dev Terr UMR1248, F-31326 Castanet Tolosan, France. [Martre, Pierre] INRA, Genet Divers & Ecophysiol Cereals GDEC UMR1095, F-63100 Clermont Ferrand, France. [Martre, Pierre] Univ Blaise Pascal, GDEC UMR1095, F-63170 Clermont Ferrand, France. [Hatfield, Jerry L.] Natl Lab Agr & Environm, Ames, IA 50011 USA. [Rosenzweig, Cynthia; Ruane, Alex C.; Goldberg, Richie] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Thorburn, Peter J.] CSIRO, Ecosyst Sci, Dutton Pk, Qld 4102, Australia. [Kersebaum, Kurt Christian; Nendel, Claas] Leibniz Ctr Agr Landscape Res, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Aggarwal, Pramod K.] CIMMYT, CGIAR Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Basso, Bruno] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. [Basso, Bruno] Michigan State Univ, Kellogg Biol Stn, E Lansing, MI 48824 USA. [Bertuzzi, Patrick; Ripoche, Dominique] INRA, AgroClim US1116, F-84914 Avignon, France. [Biernath, Christian; Priesack, Eckart] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Soil Ecol, D-85764 Neuherberg, Germany. [Brisson, Nadine] INRA, Agron UMR0211, F-78750 Thiverval Grignon, France. [Brisson, Nadine] AgroParisTech, Agron UMR0211, F-78750 Thiverval Grignon, France. [Challinor, Andrew J.] Univ Leeds, Inst Climate & Atmospher Sci, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England. [Challinor, Andrew J.] CIAT, CGIAR ESSP Program Climate Change Agr & Food Secu, Cali 6713, Colombia. [Doltra, Jordi] Cantabrian Agr Res & Training Ctr CIFA, Muriedas 39600, Spain. [Gayler, Sebastian] Univ Tubingen, Water & Earth Syst Sci Competence Cluster, D-72074 Tubingen, Germany. [Heng, Lee] IAEA, A-1400 Vienna, Austria. [Hooker, Josh] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Hooker, Josh] Joint Res Ctr, Via Enrico Fermi 2749, I-21027 Ispra, Italy. [Hunt, Leslie A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Ingwersen, Joachim; Streck, Thilo] Univ Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Izaurralde, Roberto C.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Izaurralde, Roberto C.] Texas A&M Univ, Texas A&M AgriLife Res & Extens Ctr, Temple, TX 76502 USA. [Mueller, Christoph; Waha, Katharina] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Kumar, Soora Naresh] Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [O'Leary, Garry J.] Dept Econ Dev Jobs Transport & Resources, Landscape & Water Sci, Horsham, Vic 3400, Australia. [Olesen, Jorgen E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Osborne, Tom M.] Univ Reading, Dept Meteorol, Natl Ctr Atmospher Sci, Reading RG6 6BB, Berks, England. [Semenov, Mikhail A.; Stratonovitch, Pierre] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Steduto, Pasquale] Food & Agr Org United Nations FAO, Rome, Italy. [Shcherbak, Iurii; Stockle, Claudio O.] Washington State Univ, Biol Syst Engn, Pullman, WA 99164 USA. [Supit, Iwan] Wageningen Univ, Water Syt & Global Change Grp, NL-6700 AP Wageningen, Netherlands. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Travasso, Maria] INTA CIRN, Inst Climate & Water, RA-1712 Castelar, Argentina. [White, Jeffrey W.] USDA ARS, ALARC, Maricopa, AZ USA. [Wolf, Joost] Wageningen Univ, Plant Prod Syst, NL-6700 AA Wageningen 37, Netherlands. [Rotter, Reimund P.] Univ Gottingen, Dept Crop Sci, Gottingen, Germany. [Ewert, Frank] Leibniz Ctr Agr Landscape Res ZALF, D-15374 Muncheberg, Germany. [Cammarano, Davide] James Hutton Inst, Dundee DD2 5DA, Scotland. [Martre, Pierre] INRA, Montpellier SupAgro, Lab Ecophysiol Plantes Stress Environm UMR759, F-34060 Montpellier, France. [Waha, Katharina] CSIRO, Agr, 306 Carmody Rd, St Lucia, Qld 4067, Australia. RP Cammarano, D (reprint author), Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA.; Cammarano, D (reprint author), James Hutton Inst, Dundee DD2 5DA, Scotland. EM Davide.Cammarano@hutton.ac.uk RI Palosuo, Taru/B-9593-2012; Thorburn, Peter/A-6884-2011; Doltra, Jordi/C-2106-2015; Challinor, Andrew/C-4992-2008 OI Palosuo, Taru/0000-0003-4322-3450; Challinor, Andrew/0000-0002-8551-6617 FU Ministry of Science, Research and Arts of Baden-Wurttemberg [AZ Zu 33-721.3-2]; Helmholtz Center for Environmental Research, Leipzig; European FACCE MACSUR project through the Finnish Ministry of Agriculture and Forestry; INRA Environment and Agronomy Division; framework of JPI FACCE MACSUR project through the INRA Metaprogram on the Adaptation of Agriculture and Forests to Climate Change; German Federal Office for Agriculture and Food; FACCE MACSUR [2812ERA147]; COST [ES1106]; KULUNDA [01LL0905L]; FACCE MACSUR through the German Federal Ministry of Education and Research (BMBF) [031A103B]; project of Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA) from National Institute for Food and Agriculture [2011-68002-30191] FX We thank the anonymous referees for the valuable comments and suggestions that helped improve the manuscript. S.G. was supported by a grant from the Ministry of Science, Research and Arts of Baden-Wurttemberg (AZ Zu 33-721.3-2) and the Helmholtz Center for Environmental Research, Leipzig (UFZ); R.P.R., T.P. and F.T. were supported by funds from the European FACCE MACSUR project through the Finnish Ministry of Agriculture and Forestry; P.M., P.B., N.B. and D.R. were supported by INRA Environment and Agronomy Division and by the funding within the framework of JPI FACCE MACSUR project through the INRA Metaprogram on the Adaptation of Agriculture and Forests to Climate Change; K.C.K. and C.N. received support from the German Federal Office for Agriculture and Food with FACCE MACSUR (2812ERA147) and from COST ES1106; C.M. acknowledges financial support from the KULUNDA project (01LL0905L) and the FACCE MACSUR project (031A103B) funded through the German Federal Ministry of Education and Research (BMBF); C.O.S. was supported by the project of Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA) funded through award #2011-68002-30191 from the National Institute for Food and Agriculture. This work has been carried out under the framework of the Agricultural Model Inter comparison and Improvment Project (AgMIP). NR 67 TC 2 Z9 2 U1 48 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD NOV PY 2016 VL 198 BP 80 EP 92 DI 10.1016/j.fcr.2016.08.015 PG 13 WC Agronomy SC Agriculture GA EA2FX UT WOS:000386409300009 ER PT J AU Kalsbeek, A Veenstra, J Westra, J Disselkoen, C Smith, C Tintle, N AF Kalsbeek, Anya Veenstra, Jenna Westra, Jason Disselkoen, Craig Smith, Caren Tintle, Nathan TI Genome-Wide Interaction Study of Red-Blood Cell Fatty Acids on Inflammatory Biomarkers in the Framingham Heart Study SO GENETIC EPIDEMIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the International-Genetic-Epidemiology-Society CY OCT 24-26, 2016 CL Toronto, CANADA SP Int Genet Epidemiol Soc C1 [Kalsbeek, Anya; Veenstra, Jenna; Westra, Jason; Disselkoen, Craig; Tintle, Nathan] Dordt Coll, Dept Math Stat & Comp Sci, Sioux Ctr, IA USA. [Smith, Caren] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0741-0395 EI 1098-2272 J9 GENET EPIDEMIOL JI Genet. Epidemiol. PD NOV PY 2016 VL 40 IS 7 MA 97 BP 644 EP 644 PG 1 WC Genetics & Heredity; Mathematical & Computational Biology SC Genetics & Heredity; Mathematical & Computational Biology GA DZ7HF UT WOS:000386034800103 ER PT J AU Grant, MR Tymon, LS Helms, GL Thomashow, LS Keller, CK Harsh, JB AF Grant, M. R. Tymon, L. S. Helms, G. L. Thomashow, L. S. Keller, C. Kent Harsh, J. B. TI Biofilm adaptation to iron availability in the presence of biotite and consequences for chemical weathering SO GEOBIOLOGY LA English DT Article ID PSEUDOMONAS-AERUGINOSA; MICROBIAL POLYSACCHARIDES; BACTERIAL COMMUNITIES; FELDSPAR DISSOLUTION; NMR-SPECTROSCOPY; INHIBITION; CORROSION; MINERALS; SURFACES; METALS AB Bacteria in nature often live within biofilms, exopolymeric matrices that provide a favorable environment that can differ markedly from their surroundings. Biofilms have been found growing on mineral surfaces and are expected to play a role in weathering those surfaces, but a clear understanding of how environmental factors, such as trace-nutrient limitation, influence this role is lacking. Here, we examine biofilm development by Pseudomonas putida in media either deficient or sufficient in Fe during growth on biotite, an Fe rich mineral, or on glass. We hypothesized that the bacteria would respond to Fe deficiency by enhancing biotite dissolution and by the formation of binding sites to inhibit Fe leaching from the system. Glass coupons acted as a no-Fe control to investigate whether biofilm response depended on the presence of Fe in the supporting solid. Biofilms grown on biotite, as compared to glass, had significantly greater biofilm biomass, specific numbers of viable cells (SNVC), and biofilm cation concentrations of K, Mg, and Fe, and these differences were greater when Fe was deficient in the medium. Scanning electron microscopy (SEM) confirmed that biofilm growth altered the biotite surface, smoothing the rough, jagged edges of channels scratched by hand on the biotite, and dissolving away small, easy-to-access particles scattered across the planar surface. High-resolution magic angle spinning proton nuclear magnetic resonance (HRMAS H-1 NMR) spectroscopy showed that, in the Fe-deficient medium, the relative amount of polysaccharide nearly doubled relative to that in biofilms grown in the medium amended with Fe. The results imply that the bacteria responded to the Fe deficiency by obtaining Fe from biotite and used the biofilm matrix to enhance weathering and as a sink for released cation nutrients. These results demonstrate one mechanism by which biofilms may help soil microbes overcome nutrient deficiencies in oligotrophic systems. C1 [Grant, M. R.; Harsh, J. B.] Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. [Tymon, L. S.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Helms, G. L.] Washington State Univ, Nucl Magnet Resonance Spect Ctr, Pullman, WA 99164 USA. [Thomashow, L. S.] Washington State Univ, Root Dis & Biol Control Res Unit, USDA ARS, Pullman, WA 99164 USA. [Keller, C. Kent] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. RP Grant, MR (reprint author), Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. EM michael.grant@wsu.edu RI Harsh, James/C-7455-2014 OI Harsh, James/0000-0002-0177-3342 FU NSF/EAR grant [0952399] FX Thanks are due to Jeff Boyle and Dr. Scott Boroughs both at Washington State University for their analytical assistance and to Dr. Tarah Sullivan for her careful critique of this manuscript. This research was financially supported by NSF/EAR grant No. 0952399 to C. Kent Keller and collaborators. Thanks are also due to the staff at the Washington State University Franceschi Microscopy and Imaging Center for their help using the SEM. I would also like to thank my soil chemistry and physics laboratory group at Washington State University who helped guide me in my journey to make this project a reality. NR 80 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1472-4677 EI 1472-4669 J9 GEOBIOLOGY JI Geobiology PD NOV PY 2016 VL 14 IS 6 BP 588 EP 598 DI 10.1111/gbi.12187 PG 11 WC Biology; Environmental Sciences; Geosciences, Multidisciplinary SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Geology GA DZ7EF UT WOS:000386026800005 PM 27384343 ER PT J AU Jenerette, GD Clarke, LW Avolio, ML Pataki, DE Gillespie, TW Pincetl, S Nowak, DJ Hutyra, LR McHale, M McFadden, JP Alonzo, M AF Jenerette, G. Darrel Clarke, Lorraine W. Avolio, Meghan L. Pataki, Diane E. Gillespie, Thomas W. Pincetl, Stephanie Nowak, Dave J. Hutyra, Lucy R. McHale, Melissa McFadden, Joseph P. Alonzo, Michael TI Climate tolerances and trait choices shape continental patterns of urban tree biodiversity SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE Biodiversity; ecosystem service; environmental filter; macroecology; tree; plant trait; urban ID SPECIES RICHNESS; PLANT DIVERSITY; LOS-ANGELES; CITIES; URBANIZATION; FLORAS; FORESTS; GARDENS; HOMOGENIZATION; COMMUNITIES AB AimWe propose and test a climate tolerance and trait choice hypothesis of urban macroecological variation in which strong filtering associated with low winter temperatures restricts urban biodiversity while weak filtering associated with warmer temperatures and irrigation allows dispersal of species from a global source pool, thereby increasing urban biodiversity. LocationTwenty cities across the USA and Canada. MethodsWe examined variation in tree community taxonomic diversity, origins and production of an aesthetic ecosystem service trait in a cross-section of urban field surveys. We correlated urban tree community composition indicators with a key climate restriction, namely mean minimum winter temperature, and evaluated alternative possible drivers: precipitation, summer maximum temperature, population size and the percentage of adults with a college education. ResultsSpecies accumulation curves differed substantially among cities, with observed richness varying from 22 to 122 species. Similarities in tree communities decreased exponentially with increases in climatic differences. Ordination of tree communities showed strong separation among cities with component axes correlated with minimum winter temperature and annual precipitation. Variation among urban tree communities in richness, origins and the provisioning of an aesthetic ecosystem service were all correlated with minimum winter temperature. Main conclusionsThe urban climate tolerance and trait choice hypothesis provides a coherent mechanism to explain the large variation among urban tree communities resulting from an interacting environment, species and human decisions. Reconciling the feedbacks between human decision making and biophysical limitations provides a foundation for an urban ecological theory that can better understand and predict the dynamics of other linked biotic communities, associated ecosystem dynamics and resulting services provided to urban residents. C1 [Jenerette, G. Darrel; Clarke, Lorraine W.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92512 USA. [Avolio, Meghan L.; Pataki, Diane E.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. [Gillespie, Thomas W.; Pincetl, Stephanie] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90095 USA. [Nowak, Dave J.] US Forest Serv, Northern Res Stn, Syracuse, NY 13210 USA. [Hutyra, Lucy R.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [McHale, Melissa] North Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [McFadden, Joseph P.; Alonzo, Michael] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Clarke, Lorraine W.] Univ Dist Columbia, Washington, DC USA. [Avolio, Meghan L.] Natl Socioenvironm Synth Ctr, Annapolis, MD USA. RP Jenerette, GD (reprint author), Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. EM darrel.jenerette@ucr.edu FU National Science Foundation [DEB-0919006, DEB-0919381, DEB-0918905, GEO-0814692, BCS-1026865, DEB-1149471, BCS-0948914]; USFS; National Aeronautic and Space Administration [NNG04GN80G]; USDA FX This work was supported by the National Science Foundation through DEB-0919006; DEB-0919381; DEB-0918905; GEO-0814692; BCS-1026865; DEB-1149471; BCS-0948914; USFS, National Aeronautic and Space Administration through NNG04GN80G, and USDA. We appreciate the help of the many research teams who helped collect the tree sample data. We thank Jeff Diez, Karl Evans, Sharon Hall and Julie Ripplinger for insightful discussions and comments on our manuscript and Jun Yang for readily sharing data. NR 60 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD NOV PY 2016 VL 25 IS 11 BP 1367 EP 1376 DI 10.1111/geb.12499 PG 10 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA EA1WJ UT WOS:000386382500009 ER PT J AU O'Donnell, K Sutton, DA Wiederhold, N Robert, VARG Crous, PW Geiser, DM AF O'Donnell, Kerry Sutton, Deanna A. Wiederhold, Nathan Robert, Vincent A. R. G. Crous, Pedro W. Geiser, David M. TI Veterinary Fusarioses within the United States SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID SOLANI SPECIES COMPLEX; MOLECULAR PHYLOGENETIC DIVERSITY; DNA-SEQUENCE DATABASE; CONTACT-LENS; INFECTIONS; MYCOSIS; MEMBERS; ORIGIN; FUNGUS; COMMON AB Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67 Fusarium strains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within the Fusarium solani species complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme, Fusarium keratoplasticum, and Fusarium sp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of the F. falciforme strains (12/15) were recovered from equine keratitis infections; however, strains of F. keratoplasticum and Fusarium sp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that the Fusarium incarnatum-equiseti species complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance within Fusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and the Fusarium oxysporum species complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes. C1 [O'Donnell, Kerry] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Peoria, IL USA. [Sutton, Deanna A.; Wiederhold, Nathan] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA. [Robert, Vincent A. R. G.; Crous, Pedro W.] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP O'Donnell, K (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Peoria, IL USA. EM kerry.odonnell@ars.usda.gov NR 41 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD NOV PY 2016 VL 54 IS 11 BP 2813 EP 2819 DI 10.1128/JCM.01607-16 PG 7 WC Microbiology SC Microbiology GA DZ8ID UT WOS:000386113200027 PM 27605713 ER PT J AU Taylor, MK Lankau, RA Wurzburger, N AF Taylor, Melanie K. Lankau, Richard A. Wurzburger, Nina TI Mycorrhizal associations of trees have different indirect effects on organic matter decomposition SO JOURNAL OF ECOLOGY LA English DT Article DE arbuscular mycorrhizas; biogeochemistry; carbon; decomposition; ectomycorrhizas; nitrogen; plant-soil relationship; soil organic matter ID LITTER DECOMPOSITION; CARBON SEQUESTRATION; ECTOMYCORRHIZAL COLONIZATION; ACQUISITION STRATEGIES; ROOT DECOMPOSITION; TEMPERATE FOREST; BOREAL FOREST; SOIL; FUNGI; NITROGEN AB Organic matter decomposition is the main process by which carbon (C) is lost from terrestrial ecosystems, and mycorrhizal associations of plants (i.e. arbuscular mycorrhizas (AM) and ectomycorrhizas (ECM)) may have different indirect effects on this loss pathway. AM and ECM plants differ in the soil decomposers they promote and the quality of litter they produce, which may result in different patterns of organic matter decomposition, and hence, soil C loss. To determine how mycorrhizal associations indirectly affect decomposer activity, we collected soils and litters from four AM and four ECM tree species from a mixed-deciduous temperate forest for a field and laboratory study. We first characterized insitu patterns in soil chemistry and soil microbial biomass among these eight tree species. We then conducted a microcosm experiment with mineral soils, leaf litter and fine roots originating from these tree species, where we reciprocally crossed litters and soils, and quantified the rate of heterotrophic respiration over a 140-day laboratory incubation. In natural forest conditions, AM tree soils contained lower total C and microbial biomass C:N relative to ECM tree soils. In our microcosm experiment, AM soils supported greater heterotrophic respiration than did ECM soils. The addition of AM litter stimulated respiration more than did ECM litter, owing to the lower C:N of AM litter. Matching the mycorrhizal identity of litter and soil resulted in a difference in total respiration, such that combinations of AM litters with AM soils lost more C than did combinations of ECM litters with ECM soils.Synthesis. Our findings demonstrate that AM and ECM trees have differing indirect effects on soil decomposer activity through the decomposers they cultivate and/or the quality of organic matter they produce. Mycorrhizal differences in litter quality accentuate these effects on soil C loss and may explain patterns in soil C dynamics in terrestrial ecosystems. C1 [Taylor, Melanie K.; Wurzburger, Nina] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Lankau, Richard A.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Taylor, Melanie K.] USDA Forest Serv, So Res Stn, Athens, GA 30602 USA. RP Wurzburger, N (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. EM ninawurz@uga.edu NR 51 TC 2 Z9 2 U1 57 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD NOV PY 2016 VL 104 IS 6 BP 1576 EP 1584 DI 10.1111/1365-2745.12629 PG 9 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DZ5PY UT WOS:000385915200007 ER PT J AU Waller, LP Callaway, RM Klironomos, JN Ortega, YK Maron, JL AF Waller, Lauren P. Callaway, Ragan M. Klironomos, John N. Ortega, Yvette K. Maron, John L. TI Reduced mycorrhizal responsiveness leads to increased competitive tolerance in an invasive exotic plant SO JOURNAL OF ECOLOGY LA English DT Article DE Arbuscular mycorrhizal fungi; Centaurea solstitialis; competition; invasion; mycorrhizal responsiveness; Stipa pulchra ID VESICULAR-ARBUSCULAR MYCORRHIZAE; TALLGRASS PRAIRIE; GROWTH-RESPONSES; SOIL DISTURBANCE; CARBON TRANSFER; C-14 TRANSFER; FUNGI; EVOLUTION; DIVERSITY; PHOSPHORUS AB Arbuscular mycorrhizal (AM) fungi can exert a powerful influence on the outcome of plant-plant competition. Since some exotic plants interact differently with soil biota such as AM fungi in their new range, range-based shifts in AM responsiveness could shift competitive interactions between exotic and resident plants, although this remains poorly studied. We explored whether genotypes of the annual exotic Centaurea solstitialis (yellow starthistle), collected from populations across the native and non-native ranges, differed in responsiveness to AM fungi in the introduced range and whether range-based differences in mycorrhizal responsiveness affected how strongly C. solstitialis tolerated competition with the North American native bunchgrass, Stipa pulchra. Grown alone, C. solstitialis from both ranges derived only weak benefits from AM fungi. However, association with AM fungi was costly to plants when grown in competition with S. pulchra. The magnitude of the suppressive effect of AM fungi was greater for genotypes from native versus introduced populations.Synthesis. Many exotic invasive species are known to associate weakly with AM fungi, which may be beneficial in disturbed habitats where competition for resources is low. Our results indicate that reduced mycorrhizal associations may also benefit invaders in a competitive environment. Centaurea solstitialis were more strongly suppressed by established S. pulchra plants in the presence versus absence of AM fungi, but exotic genotypes were less suppressed than native genotypes. This suggests that AM fungi may contribute to invasion resistance in established native communities, but range-based shifts in the way exotic genotypes respond to AM fungal partners may counter such biotic resistance. C1 [Waller, Lauren P.; Callaway, Ragan M.; Maron, John L.] Univ Montana, Div Biol Sci, 32 Campus Dr,HS 104, Missoula, MT USA. [Callaway, Ragan M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Callaway, Ragan M.] Univ Montana, Inst Ecosyst, Missoula, MT 59812 USA. [Klironomos, John N.] Univ British Columbia Okanagan, Dept Biol, Kelowna, BC V1V 1V7, Canada. [Ortega, Yvette K.] US Dept Agr Forest Serv, Rocky Mt Res Stn, Missoula, MT 59081 USA. RP Waller, LP (reprint author), Lincoln Univ, Bioprotect Res Ctr, POB 84, Lincoln 7647, New Zealand. EM lauren.waller@lincoln.ac.nz FU NSF [DEB 0614406]; NSF EPSCoR Track-1 [EPS-1101342] FX This work was funded by an NSF grant DEB 0614406 to JLM and RMC. RMC thanks the NSF EPSCoR Track-1 EPS-1101342 (INSTEP 3) for support. We thank Jose Hierro and Dani Montesinos for help collecting seeds and Giles Thelen and Jill Gebhart Wood for help collecting soil. Tyler Albrethsen, Whitney Warren and David Hooper provided support in the glasshouse. We are very grateful to Drs Ylva Lekberg, Anna Sala and three previous anonymous referees whose helpful comments improved the manuscript. The authors have no conflicts of interest to declare. NR 68 TC 0 Z9 0 U1 39 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD NOV PY 2016 VL 104 IS 6 BP 1599 EP 1607 DI 10.1111/1365-2745.12641 PG 9 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DZ5PY UT WOS:000385915200009 ER PT J AU Alshaya, A Hunt, J Rowlands, R AF Alshaya, A. Hunt, J. Rowlands, R. TI Stresses and Strains in Thick Perforated Orthotropic Plates SO JOURNAL OF ENGINEERING MECHANICS LA English DT Article DE Holes; Thick plates; Three-dimensional stresses; Three-dimensional strains; Constraint factors; Orthotropy; Sitka Spruce; Carbon; epoxy composite ID ELASTIC PLATE; HOLE; FIELD AB Stress and strain concentrations and in-plane and out-of-plane stress constraint factors associated with a circular hole in thick, loaded orthotropic composite plates are determined by three-dimensional finite element method. The plate has essentially infinite in-plane geometry but finite thickness. Results for Sitka spruce wood are emphasized, although some for carbon-epoxy composites are included. While some results are similar to those for isotropy, there are significant consequences due to material orthotropy. Maximum stress and strain concentration factors occur at midplane for thin plates but closer to the external traction-free surfaces for thick plates. These factors decrease as the plate surface is approached and reach lower values unrepresentative of the maximum values. Differences between the midplane and/or maximum and surface stress or strain concentration factors in Sitka spruce, range from 8% if the wood grain is parallel to the vertically applied load to 15% when the grain is perpendicular to the load. These values exceed those typically reported for isotropic materials. Stress and strain concentration factors tend to differ in magnitude from each other. The combination of high local stresses and directional strength dependency of orthotropic materials can be particularly important. That maximum stress and/or strain concentrations in thick plates occur on other than the external plate surfaces where they are most readily measured is technically significant. The E11/E22 ratio in Sitka Spruce exceeds that in the carbon composite by 60%. However, when loading parallel to the strong/stiff directions, the plane-stress tensile stress concentration factors of the two materials are comparable to each other. (C) 2016 American Society of Civil Engineers. C1 [Alshaya, A.; Rowlands, R.] Univ Wisconsin, Madison, WI 53706 USA. [Hunt, J.] USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Rowlands, R (reprint author), Univ Wisconsin, Madison, WI 53706 USA. EM alshaya@wisc.edu; jfhunt@fs.fed.us; rowlands@engr.wisc.edu FU Kuwait University graduate student scholarship FX The authors thank Andrew Mikkelson, UW-Madison, for technical discussions and help in the finite element modeling. Abdullah Alshaya was supported by a Kuwait University graduate student scholarship. NR 20 TC 0 Z9 0 U1 6 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9399 EI 1943-7889 J9 J ENG MECH JI J. Eng. Mech. PD NOV PY 2016 VL 142 IS 11 AR 04016082 DI 10.1061/(ASCE)EM.1943-7889.0001138 PG 10 WC Engineering, Mechanical SC Engineering GA EA1OU UT WOS:000386362500001 ER PT J AU Goldstein, A Foti, R Montalto, F AF Goldstein, Alisha Foti, Romano Montalto, Franco TI Effect of Spatial Resolution in Modeling Stormwater Runoff for an Urban Block SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article ID MULTIOBJECTIVE CALIBRATION; AGGREGATION AB This study assesses the role of spatial resolution in hydrologic and hydraulic models by means of two models developed at different resolutions for an urban block using the U.S. Environmental Protection Agency's stormwater management model (SWMM). The first is a high-resolution model, in which distinct elements are represented as individual hydrologic response units (HRU). The second is a low-resolution model, in which the entire block is represented as a single HRU. Flow observations during five storms were used to calibrate the models, while a separate set of six storms was used to assess their performance. The high-resolution model was found to systematically underestimate peak flows, whereas the performance of the low-resolution model was found to be dependent on the type of storm. Overall, the two models displayed similar accuracy according to most metrics, thus justifying modeling at high resolution only in those cases where a detailed representation is necessary. C1 [Goldstein, Alisha] DC Dept Energy & Environm, 1200 First St,NE 5th Floor, Washington, DC 20052 USA. [Goldstein, Alisha; Foti, Romano; Montalto, Franco] Drexel Univ, Dept Civil Architectural & Environm Engn, Curtis Hall Room 251,3141 Chestnut St, Philadelphia, PA 19104 USA. [Montalto, Franco] US Forest Serv, New York City Urban Field Stn, Bayside, NY 11359 USA. RP Montalto, F (reprint author), Drexel Univ, Dept Civil Architectural & Environm Engn, Curtis Hall Room 251,3141 Chestnut St, Philadelphia, PA 19104 USA.; Montalto, F (reprint author), US Forest Serv, New York City Urban Field Stn, Bayside, NY 11359 USA. EM aeg2107@gmail.com; rf376@drexel.edu; Fam26@drexel.edu FU New York State Office of the Attorney General FX The authors would like to acknowledge Omar Khader and Kim DiGiovanni for conducting the initial site visits, collecting all the observed data and devising a preliminary model. Much gratitude is given to Hugh Gordon who assisted with the program code. The authors also would like to extend special thanks to New York State Office of the Attorney General for funding the research and the following co-collaborators: Youth Ministries for Peace and Justice, NYC Department of Environmental Protection, NYC Department of Parks and Recreation and HydroQual, Inc. NR 12 TC 0 Z9 0 U1 14 U2 14 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD NOV PY 2016 VL 21 IS 11 AR 06016009 DI 10.1061/(ASCE)HE.1943-5584.0001377 PG 5 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA EA1QR UT WOS:000386367400002 ER PT J AU Bautista, E Warrick, AW Schlegel, JL Thorp, KR Hunsaker, DJ AF Bautista, E. Warrick, A. W. Schlegel, J. L. Thorp, K. R. Hunsaker, D. J. TI Approximate Furrow Infiltration Model for Time-Variable Ponding Depth SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article ID 3-DIMENSIONAL ANALYSIS; IRRIGATION ADVANCE; DISC INFILTROMETER; SOLUTE TRANSPORT; WATER; FLOW; SOILS; SIMULATION; EQUATION; SURFACE AB A methodology is proposed for estimating furrow infiltration under time-variable ponding depth. The methodology approximates the solution to the two-dimensional Richards equation, and is a modification of a procedure that was originally proposed for computing infiltration under constant ponding depth. Two computational approaches were developed and tested using several combinations of soil hydraulic properties, furrow geometry, and flow depth variations. Both methods yielded solutions of reasonable and similar accuracy relative to numerical solutions of the two-dimensional Richards equation. The analysis also showed that the accuracy of the approximate model varies mostly as a function of soil hydraulic properties. The accuracy of the approximate solution can be improved with calibration. Two calibration methods were examined, one assuming that the calibration parameter varies with depth, and the other assuming a constant value. The analysis showed that latter approach, in combination with one of the proposed computational methods, reproduced the Richards equation solution more accurately. This means that a unique calibration parameter can be developed for the particular soil and geometric configuration conditions, and applied to different patterns of ponding depth variation. (C) 2016 American Society of Civil Engineers. C1 [Bautista, E.; Schlegel, J. L.; Thorp, K. R.; Hunsaker, D. J.] ARS, US Arid Land Agr Res Ctr, USDA, 21881N Cardon Ln, Maricopa, AZ 85138 USA. [Warrick, A. W.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. RP Bautista, E (reprint author), ARS, US Arid Land Agr Res Ctr, USDA, 21881N Cardon Ln, Maricopa, AZ 85138 USA. EM Eduardo.Bautista@ars.usda.gov; aww@cals.arizona.edu; James.Schlegel@ars.usda.gov; Kelly.Thorp@ars.usda.gov; Doug.Hunsaker@ars.usda.gov NR 32 TC 0 Z9 0 U1 1 U2 1 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD NOV PY 2016 VL 142 IS 11 AR 04016045 DI 10.1061/(ASCE)IR.1943-4774.0001057 PG 11 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA EA1MZ UT WOS:000386357700003 ER PT J AU Henry, CG Hirsh, SL Anders, MM Vories, ED Reba, ML Watkins, KB Hardke, JT AF Henry, C. G. Hirsh, S. L. Anders, M. M. Vories, E. D. Reba, M. L. Watkins, K. B. Hardke, J. T. TI Annual Irrigation Water Use for Arkansas Rice Production SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE Contour-levee rice irrigation; Multiple inlet rice irrigation; Straight-levee rice irrigation; Zero-grade rice AB This study investigated rice irrigation water use in the University of Arkansas Rice Research Verification Program between the years of 2003 and 2012. Annual irrigation water use averaged 763mm (30.0in.) over 10years. A significant (40%) water savings was found for rice grown using a zero-grade irrigation system [486mm (19.1in.)] compared to contour-levee or straight-levee systems. No differences in irrigation water use were found between contour-levee systems [814mm (32.1in.)] and straight-levee systems [822mm (32.4in.)]. Surface water irrigators used significantly less irrigation water [624mm (24.6in.)] than groundwater well fields [786mm (30.7in.)] and cost US$77.51 less per hectare (US$31.37 less per acre) to operate. Diesel pumps cost significantly more [$88.38/acre ($218.29/ha)] to operate than electric pumps [$58.69/acre (145.96/ha)] (P=0.02), although fields that used diesel as an energy source averaged 152mm (6.0in.) less irrigation water than electric pumps. Arkansas producers implementing the multiple-inlet water delivery practice on contour-levee or straight-levee systems, irrespective of soil type, did not realize a water savings but did realize improved yields. These results are in contrast to those in earlier studies and suggest that an educational effort may be necessary if the field research-proven multiple-inlet water savings are to be realized. C1 [Henry, C. G.] Univ Arkansas, Rice Res & Extens Ctr, Biol & Agr Engn, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Hirsh, S. L.] Univ Maryland, Dept Environm Sci & Technol, 1443 Anim Sci Bldg, College Pk, MD 20742 USA. [Anders, M. M.] Univ Arkansas, Rice Res & Extens Ctr, Crop Soil & Environm Sci, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Vories, E. D.] Univ Missouri, Delta Ctr, USDA, ARS, POB 160,147 State Highway T, Portageville, MO 63873 USA. [Reba, M. L.] ARS, USDA, Delta Water Management Res Unit, 504 Univ Loop East, Jonesboro, AR 72401 USA. [Watkins, K. B.] Univ Arkansas, Dept Agr Econ, Rice Res & Extens Ctr, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. [Hardke, J. T.] Univ Arkansas, Rice Res & Extens Ctr, Soil & Environm Sci, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. RP Henry, CG (reprint author), Univ Arkansas, Rice Res & Extens Ctr, Biol & Agr Engn, 2900 Hwy 130 East, Stuttgart, AR 72160 USA. EM cghenry@uark.edu; mmanders@uark.edu; Earl.Vories@ars.usda.gov; michele.reba@ars.usda.gov; kbwatkins@uark.edu; jhardke@uaex.edu FU Arkansas Rice Research and Promotion Board FX The authors would like to thank Phil Tacker and all the past verification coordinators and faculty project leaders throughout the history of the RRVP program for their efforts in collecting these data. These data would not have been available without the long-term financial support of the Arkansas Rice Research and Promotion Board. NR 7 TC 0 Z9 0 U1 6 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD NOV PY 2016 VL 142 IS 11 AR 05016006 DI 10.1061/(ASCE)IR.1943-4774.0001068 PG 5 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA EA1MZ UT WOS:000386357700005 ER PT J AU Johnson, ET Dowd, PF AF Johnson, Eric T. Dowd, Patrick F. TI A quantitative method for determining relative colonization rates of maize callus by Fusarium graminearum for resistance gene evaluations SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Maize; Mycotoxin; Callus; Pathogen resistance; Peroxidase ID INSECT RESISTANCE; TISSUE-CULTURE; EXPRESSION; ROT; PLANTS AB A quantitative PCR method was developed for detecting Fusarium graminearum growing in maize callus. Fungal DNA was detected 12 h after inoculation (detection limit, 0.2 pg) and was correlated with visual ratings. The method effectively quantified fungal growth in callus overexpressing a peroxidase gene conferring fungal resistance. Published by Elsevier B.V. C1 [Johnson, Eric T.; Dowd, Patrick F.] USDA ARS, Crop Bioprotect Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Johnson, ET (reprint author), USDA ARS, Crop Bioprotect Res, 1815 N Univ St, Peoria, IL 61604 USA. EM eric.johnson2@ars.usda.gov FU ARS project [5010-42000-047-00] FX The mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. This research was supported by funds from ARS project 5010-42000-047-00. NR 18 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD NOV PY 2016 VL 130 BP 73 EP 75 DI 10.1016/j.mimet.2016.08.026 PG 3 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA EA2EZ UT WOS:000386406900011 PM 27577788 ER PT J AU Kiszonas, AM Fuerst, EP Talbert, L Sherman, J Morris, CF AF Kiszonas, Alecia M. Fuerst, E. Patrick Talbert, Luther Sherman, Jamie Morris, Craig F. TI Effect of wheat (Triticum aestivum L.) seed color and hardness genes on the consumption preference of the house mouse (Mus musculus L.) SO MAMMALIA LA English DT Article DE feeding preference; grain hardness; house mouse; seed color; wheat grain ID FOOD PREFERENCES; SPRING WHEAT; GRAIN HARDNESS; MICE; RED; KERNEL; PUROINDOLINES; REGISTRATION; VARIETIES; ENDOSPERM AB Wheat (Triticum aestivum L.) seeds are a primary food of the house mouse (Mus musculus L.) in agricultural environments. Behavioral aspects of seed selection and consumption have not been fully characterized, especially with regards to seed traits that mice select for or against. The present study used a common strain of mice (C57BL/6J) to evaluate the effect of seed coat pigmentation and hardness (texture) on wheat seed selection and consumption. Wheat grains with contrasting traits were presented to mice in binary mixtures, similar to "two-choice" tests. Mice preferred wheat grain with fewer Red pigmentation genes (similar to avoided grain with a greater number of Red genes). The mice were sensitive to small differences in grain texture, with softer texture consistently preferred. In summary, both seed color (pigmentation) and texture influence the selective consumption of wheat grain by C57BL/6J mice. Although the preference for softer texture and less pigmented grain is clear, other aspects of behavioral selection and consumption preference will require further study. C1 [Kiszonas, Alecia M.; Fuerst, E. Patrick; Morris, Craig F.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. [Talbert, Luther; Sherman, Jamie] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. RP Morris, CF (reprint author), Washington State Univ, USDA ARS, Western Wheat Qual Lab, E-202 Food Qual Bldg, Pullman, WA 99164 USA. EM morrisc@wsu.edu FU Ardent Mills LLC FX Caleb James performed the mouse feeding trials and data collection; his keen observations were invaluable to the success of this research. The staff of the WSU Vivarium are thanked. Stacey Sykes and Shawna Vogl assisted in the preparation and submission of the manuscript. Partial funding was provided by Ardent Mills LLC. NR 31 TC 0 Z9 0 U1 4 U2 4 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0025-1461 EI 1864-1547 J9 MAMMALIA JI Mammalia PD NOV PY 2016 VL 80 IS 6 BP 655 EP 662 DI 10.1515/mammalia-2015-0123 PG 8 WC Zoology SC Zoology GA EA1EU UT WOS:000386336100008 ER PT J AU von Rein, I Kayler, ZE Premke, K Gessler, A AF von Rein, I. Kayler, Z. E. Premke, K. Gessler, A. TI Desiccation of sediments affects assimilate transport within aquatic plants and carbon transfer to microorganisms SO PLANT BIOLOGY LA English DT Article DE Aquatic plant-sediment-microorganism carbon continuum; (CO2)-C-13 pulse labelling; drought stress; non-structural carbon compounds (NSCs); phospholipid fatty acids (PLFAs); Phragmites australis; stable isotopes; Typha latifolia ID COMMUNITY COMPOSITION; MICROBIAL BIOMASS; PHRAGMITES-AUSTRALIS; ISOTOPIC COMPOSITION; EXPERIMENTAL DROUGHT; TEMPERATE GRASSLAND; RICINUS-COMMUNIS; SUMMER DROUGHT; LAKE-SEDIMENTS; SOIL-MOISTURE AB With the projected increase in drought duration and intensity in future, small water bodies, and especially the terrestrial-aquatic interfaces, will be subjected to longer dry periods with desiccation of the sediment. Drought effects on the plant-sediment microorganism carbon continuum may disrupt the tight linkage between plants and microbes which governs sediment carbon and nutrient cycling, thus having a potential negative impact on carbon sequestration of small freshwater ecosystems. However, research on drought effects on the plant-sediment carbon transfer in aquatic ecosystems is scarce. We therefore exposed two emergent aquatic macrophytes, Phragmites australis and Typha latifolia, to a month-long summer drought in a mesocosm experiment. We followed the fate of carbon from leaves to sediment microbial communities with (CO2)-C-13 pulse labelling and microbial phospholipid-derived fatty acid (PLFA) analysis. We found that drought reduced the total amount of carbon allocated to stem tissues but did not delay the transport. We also observed an increase in accumulation of C-13-labelled sugars in roots and found a reduced incorporation of C-13 into the PLFAs of sediment microorganisms. Drought induced a switch in plant carbon allocation priorities, where stems received less new assimilates leading to reduced starch reserves whilst roots were prioritised with new assimilates, suggesting their use for osmoregulation. There were indications that the reduced carbon transfer from roots to microorganisms was due to the reduction of microbial activity via direct drought effects rather than to a decrease in root exudation or exudate availability. C1 [von Rein, I.; Kayler, Z. E.; Premke, K.; Gessler, A.] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany. [Kayler, Z. E.] US Forest Serv, USDA, No Res Stn, Lawrence Livermore Natl Lab, Livermore, CA USA. [Premke, K.] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Chem Analyt & Biogeochem, Berlin, Germany. [Gessler, A.] Berlin Brandenburg Inst Adv Biodivers Res BBIB, Berlin, Germany. [Gessler, A.] Swiss Fed Inst Forest Snow & Landscape Res WSL, Birmensdorf, Switzerland. RP von Rein, I (reprint author), Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany. EM isabell.rein@zalf.de RI Gessler, Arthur/C-7121-2008 OI Gessler, Arthur/0000-0002-1910-9589 FU German Research Council (DFG); Swiss National Science Foundation (SNF) FX Special thanks go to all those who helped with the harvests and sample preparations: Petra Lange, Monika Roth, Kennedy Kweku Kasta, Darline Krebel, Marco Heyde, Kai Nitzsche, Sasa Zavadlav and Marcus Fahle. We are grateful to Matthias Saurer from the PSI for his fast response and help with isotope analyses. We also want to thank Grit von der Waydbrink for her technical assistance in sugar analyses and Susanne Remus for her help in isotope analyses. AG acknowledges financial support from the German Research Council (DFG) and from the Swiss National Science Foundation (SNF). NR 68 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1435-8603 EI 1438-8677 J9 PLANT BIOLOGY JI Plant Biol. PD NOV PY 2016 VL 18 IS 6 BP 947 EP 961 DI 10.1111/plb.12486 PG 15 WC Plant Sciences SC Plant Sciences GA EA1KG UT WOS:000386350400009 PM 27465780 ER PT J AU Sandhu, D Atkinson, T Noll, A Johnson, C Espinosa, K Boelter, J Abel, S Dhatt, BK Barta, T Singsaas, E Sepsenwol, S Goggi, AS Palmer, RG AF Sandhu, Devinder Atkinson, Taylor Noll, Andrea Johnson, Callie Espinosa, Katherine Boelter, Jessica Abel, Stephanie Dhatt, Balpreet K. Barta, Terese Singsaas, Eric Sepsenwol, Sol Goggi, A. Susana Palmer, Reid G. TI Soybean proteins GmTic110 and GmPsbP are crucial for chloroplast development and function SO PLANT SCIENCE LA English DT Article DE Soybean; Chlorophyll-deficient; Photosynthesis; Tic110; PsbP ID CHLOROPHYLL-DEFICIENT MUTANT; IMPORT MACHINERY; OXYGEN EVOLUTION; PHOTOSYSTEM-II; HIGHER-PLANTS; BINDING-SITE; GLYCINE-MAX; GENE; LINKAGE; IDENTIFICATION AB We have identified a viable-yellow and a lethal-yellow chlorophyll-deficient mutant in soybean. Segregation patterns suggested single-gene recessive inheritance for each mutant. The viable- and lethal-yellow plants showed significant reduction of chlorophyll a and b. Photochemical energy conversion efficiency and photochemical reflectance index were reduced in the viable-yellow plants relative to the wild type, whereas the lethal-yellow plants showed no electron transport activity. The viable-yellow plants displayed reduced thylakoid stacking, while the lethal-yellow plants exhibited failure of proplastid differentiation into normal chloroplasts with grana. Genetic analysis revealed recessive epistatic interaction between the viable- and the lethal-yellow genes. The viable-yellow gene was mapped to a 58 kb region on chromosome 2 that contained seven predicted genes. A frame shift mutation, due to a single base deletion in Glyma.02g233700, resulted in an early stop codon. Glyma.02g233700 encodes a translocon in the inner membrane of chloroplast (GmTic110) that plays a critical role in plastid biogenesis. The lethal-yellow gene was mapped to an 83 kb region on chromosome 3 that contained 13 predicted genes. Based on the annotated functions, we sequenced three potential candidate genes. A single base insertion in the second exon of Glyma.03G230300 resulted in a truncated protein. Glyma.03G230300 encodes for GmPsbP, an extrinsic protein of Photosystem II that is critical for oxygen evolution during photosynthesis. GmTic110 and GmPsbP displayed highly reduced expression in the viable- and lethal-yellow mutants, respectively. The yellow phenotypes in the viable- and lethal-yellow mutants were due to the loss of function of GmTic110 or GmPsbP resulting in photooxidative stress. Published by Elsevier Ireland Ltd. C1 [Sandhu, Devinder] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Atkinson, Taylor; Noll, Andrea; Johnson, Callie; Boelter, Jessica; Abel, Stephanie; Dhatt, Balpreet K.; Barta, Terese; Singsaas, Eric; Sepsenwol, Sol] Univ Wisconsin, Dept Biol, Stevens Point, WI 54481 USA. [Espinosa, Katherine; Goggi, A. Susana; Palmer, Reid G.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Dhatt, Balpreet K.] Punjab Agr Univ, Dept Plant Breeding & Genet, Ludhiana, Punjab, India. [Singsaas, Eric] Univ Minnesota, Nat Resources Res Inst, Duluth, MN 55811 USA. RP Sandhu, D (reprint author), 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Devinder.sandhu@ars.usda.gov; tayatkinson25@gmail.com; anoll226@gmail.com; Callie.Johnson@landmark.coop; kt.espinosa67@gmail.com; jboelt92@gmail.com; sabel728@gmail.com; dhattbalpreet03@gmail.com; tbarta@uwsp.edu; esingsaa@d.umn.edu; ssepsenw@uwsp.edu; susana@iastate.edu FU UWSP Undergraduate Education Initiative; UWSP Student Research Funds; University Personnel Development Committee funds; American Seed Trade Association's Seed Research Fund FX This research project was supported by the UWSP Undergraduate Education Initiative, the UWSP Student Research Funds and University Personnel Development Committee funds. Dr. Katherine Espinosa's graduate research was supported by the American Seed Trade Association's Seed Research Fund. Authors thank Dr. Manju Pudussery for helping with expression analysis and Dr. Jorge Ferreira for taking pictures of the plants. NR 45 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD NOV PY 2016 VL 252 BP 76 EP 87 DI 10.1016/j.plantsci.2016.07.006 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA EA2GE UT WOS:000386410000008 PM 27717480 ER PT J AU Patwa, A Ambrose, RPK Casada, ME AF Patwa, Abhay Ambrose, R. P. Kingsly Casada, Mark. E. TI Discrete element method as an approach to model the wheat milling process SO POWDER TECHNOLOGY LA English DT Article ID RHEOLOGICAL PROPERTIES; COMPRESSIVE STRENGTH; DEM; GRAINS; ENDOSPERM; PARTICLE; COMMINUTION; BREAKAGE; MOISTURE AB It is a well-known phenomenon that break-release, particle size, and size distribution of wheat milling are functions of machine operational parameters and grain properties. Due to the non-uniform characteristics and properties of wheat kernels, the kernel physical and mechanical properties affect the size reduction process. This research was the first to test the functionality of the discrete element method (DEM) to simulate the 1st break wheat roller milling process. DEM models of 1st break wheat milling were developed by incorporating the bonded particle model along with the spherical-shaped and kernel-shaped particle models. The models simulated hard red winter (HRW) wheat milling at 12%, 14%, and 16% moisture content and was validated using lab scale milling trials. The spherical-shaped approach simulated the size reduction of a spherical cluster of mono-sized particles with uniform bond strength throughout the kernel. At 16% moisture content, this spherical-shaped kernel model resulted in an average particle size of 438 mu m with a deviation of prediction of 177%. The prediction error was reduced to 144% with a mean PSD of 372 mu m by modifying the shear modulus and coefficient of restitution values. With the kernel-shaped model, a bonded cluster resembling a wheat kernel in shape and size was used with a random distribution of particle bond strengths in the kernel. Even though the model predicted a 1st break particle size of 413 mu m, which had a deviation of 139% from the lab scale milling results, the model satisfactorily predicted the variation in particle size distribution from 1st break milling with moisture content. (C) 2016 Elsevier B.V. All rights reserved. C1 [Patwa, Abhay] Mennel Milling Co, Fostoria, OH 44830 USA. [Ambrose, R. P. Kingsly] Purdue Univ, Dept Agr & Biol Engn, 225 S Univ St, W Lafayette, IN 47907 USA. [Casada, Mark. E.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Ambrose, RPK (reprint author), Purdue Univ, Dept Agr & Biol Engn, 225 S Univ St, W Lafayette, IN 47907 USA. EM rambrose@purdue.edu NR 34 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0032-5910 EI 1873-328X J9 POWDER TECHNOL JI Powder Technol. PD NOV PY 2016 VL 302 BP 350 EP 356 DI 10.1016/j.powtec.2016.08.052 PG 7 WC Engineering, Chemical SC Engineering GA EA2KF UT WOS:000386420500034 ER PT J AU Wang, ZS Erb, AM Schaaf, CB Sun, QS Liu, Y Yang, Y Shuai, YM Casey, KA Roman, MO AF Wang, Zhuosen Erb, Angela M. Schaaf, Crystal B. Sun, Qingsong Liu, Yan Yang, Yun Shuai, Yanmin Casey, Kimberly A. Roman, Miguel O. TI Early spring post-fire snow albedo dynamics in high latitude boreal forests using Landsat-8 OLI data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat-8 snow albedo; Post-fire recovery; Albedo heterogeneity and dynamics ID LAND-COVER DATABASE; BURN SEVERITY; INTERIOR ALASKA; BLACK SPRUCE; SURFACE REFLECTANCE; ENERGY-EXCHANGE; PRODUCT MCD43A; CLIMATE-CHANGE; CLOUD SHADOW; IN-SITU AB Taking advantage of the improved radiometric resolution of Landsat-8 OLI which, unlike previous Landsat sensors, does not saturate over snow, the progress of fire recovery progress at the landscape scale (<100 m) is examined. High quality Landsat-8 albedo retrievals can now capture the true reflective and layered character of snow cover over a full range of land surface conditions and vegetation densities. This new capability particularly improves the assessment of post-fire vegetation dynamics across low- to high-burn severity gradients in Arctic and boreal regions in the early spring, when the albedos during recovery show the greatest variation. We use 30 m resolution Landsat-8 surface reflectances with concurrent coarser resolution (500 m) MODIS high quality full inversion surface Bidirectional Reflectance Distribution Functions (BRDF) products to produce higher resolution values of surface albedo. The high resolution full expression shortwave blue sky albedo product performs well with an overall RMSE of 0.0267 between tower and satellite measures under both snow-free and snow-covered conditions. While the importance of post-fire albedo recovery can be discerned from the MODIS albedo product at regional and global scales, our study addresses the particular importance of early spring post-fire albedo recovery at the landscape scale by considering the significant spatial heterogeneity of burn severity, and the impact of snow on the early spring albedo of various vegetation recovery types. We found that variations in early spring albedo within a single MODIS gridded pixel can be larger than 0.6. Since the frequency and severity of wildfires in Arctic and boreal systems is expected to increase in the coming decades, the dynamics of albedo in response to these rapid surface changes will increasingly impact the energy balance and contribute to other climate processes and physical feedback mechanisms. Surface radiation products derived from Landsat-8 data will thus play an important role in characterizing the carbon cycle and ecosystem processes of high latitude systems. (C) 2016 Elsevier Inc. All rights reserved. C1 [Wang, Zhuosen; Casey, Kimberly A.; Roman, Miguel O.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Wang, Zhuosen; Erb, Angela M.; Schaaf, Crystal B.; Sun, Qingsong; Liu, Yan; Shuai, Yanmin] Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. [Wang, Zhuosen] Goddard Space Flight Ctr, Greenbelt, MD USA. [Yang, Yun] ARS, USDA, Beltsville, MD USA. [Casey, Kimberly A.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Wang, ZS (reprint author), NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA.; Erb, AM (reprint author), Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. EM zhuosen.wang@nasa.gov; Angela.Erb001@umb.edu OI Sun, Qingsong/0000-0002-7710-2123 FU NASA [NNX14A173G]; USGS [G12PC00072]; Office of Science (BER), US Department of Energy through the Ameriflux Management Project FX This research was supported by NASA awards NNX14A173G and USGS award G12PC00072. The MODIS data were obtained from the NASA Distributed Active Archive Centers (DAACs). The Landsat data were obtained from the USGS Earth Resources Observation and Science (EROS) Center Science Processing Architecture (ESPA). Field albedo measurements were downloaded from NOAA SURFRAD. We gratefully acknowledge Dr. Adrian Rocha from University of Notre Dame for providing ground albedo measurements at the Imnavait site, Dr. David Y. Hollinger from USDA Forest Service Northern Research Station, Durham, NH, USA for providing ground albedo measurements at the Howland West site and Dr. Kimberly Novick and Dr. Benjamin Sulman from Indiana University for providing ground albedo measurements at the Morgan Monroe State Forest site which was supported primarily by the Office of Science (BER), US Department of Energy through the Ameriflux Management Project. NR 91 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 71 EP 83 DI 10.1016/j.rse.2016.02.059 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900007 ER PT J AU Semmens, KA Anderson, MC Kustas, WP Gao, F Alfieri, JG McKee, L Prueger, JH Hain, CR Cammalleri, C Yang, Y Xia, T Sanchez, L Alsina, MM Velez, M AF Semmens, Kathryn A. Anderson, Martha C. Kustas, William P. Gao, Feng Alfieri, Joseph G. McKee, Lynn Prueger, John H. Hain, Christopher R. Cammalleri, Carmelo Yang, Yun Xia, Ting Sanchez, Luis Alsina, Maria Mar Velez, Monica TI Monitoring daily evapotranspiration over two California vineyards using Landsat 8 in a multi-sensor data fusion approach SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat 8; Evapotranspiration; Vineyard; MODIS; Fusion; Energy fluxes ID SURFACE-ENERGY FLUXES; MAPPING DAILY EVAPOTRANSPIRATION; SPATIAL-RESOLUTION; MODEL PERFORMANCE; SATELLITE IMAGERY; 2-SOURCE MODEL; WATER-CONTENT; TIME-SERIES; SCALES; BALANCE AB California's Central Valley grows a significant fraction of grapes used for wine production in the United States. With increasing vineyard acreage, reduced water availability in much of California, and competing water use interests, it is critical to be able to monitor regional water use and evapotranspiration (ET) over large areas, but also in detail at individual field scales to improve water management within these viticulture production systems. This can be achieved by integrating remote sensing data from multiple satellite systems with different spatiotemporal characteristics. In this research, we evaluate the utility of a multi-scale system for monitoring ET as applied over two vineyard sites near Lodi, California during the 2013 growing season, leading into the drought in early 2014. The system employs a multi-sensor satellite data fusion methodology (STARFM: Spatial and Temporal Adaptive Reflective Fusion Model) combined with a multi-scale ET retrieval algorithm based on the Two Source Energy Balance (TSEB) land-surface representation to compute daily ET at 30 m resolution. In this system, TSEB is run using thermal band imagery from the Geostationary Environmental Operational Satellites (GOES; 4 km spatial resolution, hourly temporal sampling), the Moderate Resolution Imaging Spectroradiometer (MODIS) data (1 km resolution, daily acquisition) and the new Landsat 8 satellite (sharpened to 30 m resolution, similar to 16 day acquisition). Estimates of daily ET generated in two neighboring fields of Pinot noir vines of different age agree with ground-based flux measurements acquired in-field during most of the 2013 season with relative mean absolute errors on the order of 19-23% (root mean square errors of approximately 1 mm d(-1)), reducing to 14-20% at the weekly timestep relevant for irrigation management (similar to 5 mm wk(-1)). A model overestimation of ET in the early season was detected in the younger vineyard, perhaps relating to an inter-row grass cover crop. Spatial patterns of cumulative ET generally correspond to measured yield maps and indicate areas of variable crop moisture, soil condition, and yield within the vineyards that could require adaptive management. The results suggest that multi-sensor remote sensing observations provide a unique means for monitoring crop water use and soil moisture status at field-scales over extended growing regions, and may have value in supporting operational water management decisions in vineyards and other high value crops. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. C1 [Semmens, Kathryn A.; Anderson, Martha C.; Kustas, William P.; Gao, Feng; Alfieri, Joseph G.; McKee, Lynn; Yang, Yun] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Prueger, John H.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Cammalleri, Carmelo] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, Climate Risk Management Unit, Ispra, Italy. [Xia, Ting] Tsinghua Univ, Beijing, Peoples R China. [Sanchez, Luis; Alsina, Maria Mar] E&J Gallo Winery Viticulture Res, Modesto, CA USA. [Velez, Monica] Univ Puerto Rico Rio Piedras, USDA, Hispan Serving Inst Natl Program, San Juan, PR USA. RP Semmens, KA (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM kalese@gmail.com RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU USDA Natural Resources Career Track-Puerto Rico/USDA Hispanic Serving Institutions National Program-Preparing Students For Career Paths With The USDA Forest Service By Linking Student Success With Experiential Learning Opportunities; Ernest and Julio Gallo Winery FX We would like to acknowledge the support for the internship of Monica Velez from the USDA Natural Resources Career Track-Puerto Rico/USDA Hispanic Serving Institutions National Program-Preparing Students For Career Paths With The USDA Forest Service By Linking Student Success With Experiential Learning Opportunities. Funding provided by Ernest and Julio Gallo Winery made possible the acquisition and processing of the high resolution aircraft imagery used in this study. In addition we would like to thank the staff of Viticulture, Chemistry and Enology Division of Ernest and Julio Gallo Winery, in particular Nick Dokooozlian, Luis Sanchez, and Martin Mendez-Costabel who made possible the collection of the field validation data. The US Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 65 TC 6 Z9 6 U1 33 U2 33 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 155 EP 170 DI 10.1016/j.rse.2015.10.025 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900014 ER PT J AU Huntington, J McGwire, K Morton, C Snyder, K Peterson, S Erickson, T Niswonger, R Carroll, R Smith, G Allen, R AF Huntington, Justin McGwire, Kenneth Morton, Charles Snyder, Keirith Peterson, Sarah Erickson, Tyler Niswonger, Richard Carroll, Rosemary Smith, Guy Allen, Richard TI Assessing the role of climate and resource management on groundwater dependent ecosystem changes in arid environments with the Landsat archive SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; NDVI; Cross-sensor calibration; Groundwater dependent ecosystems; Riparian restoration; Groundwater pumping; Phreatophytes; Complementary relationship; Evaporative demand ID WATER-TABLE DRAWDOWN; GREATER SAGE-GROUSE; RIPARIAN VEGETATION; SURFACE REFLECTANCE; FOREST DISTURBANCE; DETECTING TRENDS; EVAPOTRANSPIRATION; MODEL; BASIN; RESTORATION AB Groundwater dependent ecosystems (GDEs) rely on near-surface groundwater. These systems are receiving more attention with rising air temperature, prolonged drought, and where groundwater pumping captures natural groundwater discharge for anthropogenic use. Phreatophyte shrublands, meadows, and riparian areas are GDEs that provide critical habitat for many sensitive species, especially in arid and semi-arid environments. While GDEs are vital for ecosystem services and function, their long-term (i.e. similar to 30 years) spatial and temporal variability is poorly understood with respect to local and regional scale climate, groundwater, and rangeland management. In this work, we compute time series of NDVI derived from sensors of the Landsat TM, ETM +, and OLI lineage for assessing GDEs in a variety of land and water management contexts. Changes in vegetation vigor based on climate, groundwater availability, and land management in arid landscapes are detectable with Landsat. However, the effective quantification of these ecosystem changes can be undermined if changes in spectral bandwidths between different Landsat sensors introduce biases in derived vegetation indices, and if climate, and land and water management histories are not well understood. The objective of this work is to 1) use the Landsat 8 under-fly dataset to quantify differences in spectral reflectance and NDVI between Landsat 7 ETM + and Landsat 8 OLI for a range of vegetation communities in arid and semiarid regions of the southwestern United States, and 2) demonstrate the value of 30-year historical vegetation index and climate datasets for assessing GDEs. Specific study areas were chosen to represent a range of GDEs and environmental conditions important for three scenarios: baseline monitoring of vegetation and climate, riparian restoration, and groundwater level changes. Google's Earth Engine cloud computing and environmental monitoring platform is used to rapidly access and analyze the Landsat archive along with downscaled North American Land Data Assimilation System gridded meteorological data, which are used for both atmospheric correction and correlation analysis. Results from the cross-sensor comparison indicate a benefit from the application of a consistent atmospheric correction method, and that NDVI derived from Landsat 7 and 8 are very similar within the study area. Results from continuous Landsat time series analysis clearly illustrate that there are strong correlations between changes in vegetation vigor, precipitation, evaporative demand, depth to groundwater, and riparian restoration. Trends in summer NDVI associated with riparian restoration and groundwater level changes were found to be statistically significant, and interannual summer NDVI was found to be moderately correlated to interannual water-year precipitation for baseline study sites. Results clearly highlight the complementary relationship between water-year PPT, NDVI, and evaporative demand, and are consistent with regional vegetation index and complementary relationship studies. This work is supporting land and water managers for evaluation of GDEs with respect to climate, groundwater, and resource management. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license. C1 [Huntington, Justin; McGwire, Kenneth; Morton, Charles; Carroll, Rosemary; Smith, Guy] Desert Res Inst, Reno, NV USA. [Huntington, Justin] Western Reg Climate Ctr, Reno, NV 89512 USA. [Snyder, Keirith] USDA ARS, Reno, NV USA. [Peterson, Sarah] Bur Land Management, Reno, NV USA. [Erickson, Tyler] Google Inc, Mountain View, CA USA. [Niswonger, Richard] US Geol Survey, 345 Middlefield Rd, Menlo Pk, CA 94025 USA. [Allen, Richard] Univ Idaho, Kimberly, ID USA. RP Huntington, J (reprint author), Western Reg Climate Ctr, Reno, NV 89512 USA. EM justin.huntington@dri.edu OI Smith, Guy/0000-0002-4579-8752 FU U.S. Bureau of Land Management [L13AC00169]; U.S. Department of Agriculture Agricultural Research Service [59-5370-3-001]; Desert Research Institute Maki Endowment; U.S. Geological Survey Landsat Science Team; Department of the Interior Northwest Climate Science Center grant; Google Earth Engine faculty research grant FX Funding for this study was provided by the U.S. Bureau of Land Management grant #L13AC00169, U.S. Department of Agriculture Agricultural Research Service grant #59-5370-3-001, Desert Research Institute Maki Endowment, U.S. Geological Survey 2012-2017 Landsat Science Team, Department of the Interior Northwest Climate Science Center grant to evaluate new strategies for addressing climate change impacts on water and ecosystems in the Great Basin, and a Google Earth Engine faculty research grant. ESPA Landsat Surface Reflectance products used were courtesy of the U.S. Geological Survey. The authors would like to thank Stephen Maples of the U.S. Geological Survey, and four anonymous reviewers for their thoughtful comments and suggestions. They greatly improved the manuscript. NR 70 TC 2 Z9 2 U1 27 U2 27 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 186 EP 197 DI 10.1016/j.rse.2016.07.004 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900016 ER PT J AU Schroeder, W Oliva, P Giglio, L Quayle, B Lorenz, E Morelli, F AF Schroeder, Wilfrid Oliva, Patricia Giglio, Louis Quayle, Brad Lorenz, Eckehard Morelli, Fabiano TI Active fire detection using Landsat-8/OLI data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; RADIATIVE ENERGY; ETM PLUS; MODIS; PRODUCT; VALIDATION; SYSTEM; ASTER; ALGORITHM AB The gradual increase in Landsat-class data availability creates new opportunities for fire science and management applications that require higher-fidelity information about biomass burning, improving upon existing coarser spatial resolution (>= 1 km) satellite active fire data sets. Targeting those enhanced capabilities we describe an active fire detection algorithm for use with Landsat-8 Operational Land Imager (OLI) daytime and nighttime data. The approach builds on the fire-sensitive short-wave infrared channel 7 complemented by visible and near-infrared channel 1-6 data (daytime only), while also expanding on the use of multi-temporal analysis to improve pixel classification results. Despite frequent saturation of OLI's fire-affected pixels, which includes radiometric artifacts resulting from folding of digital numbers, our initial assessment based on visual image analysis indicated high algorithm fidelity across a wide range of biomass burning scenarios, gas flares and active volcanoes. Additional field data verification confirmed the sensor's and algorithm's ability to resolve fires of significantly small areas compared to current operational satellite fire products. Commission errors were greatly reduced with the addition of multi-temporal analysis tests applied toco-located pixels, averaging less than 0.2% globally. Because of its overall quality, Landsat-8/OLI active fire data could become part of a network of emerging earth observation systems providing enhanced spatial and temporal coverage of biomass burning at global scales. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. C1 [Schroeder, Wilfrid; Oliva, Patricia; Giglio, Louis] Univ Maryland, Dept Geog Sci, 2181 LeFrak Hall, College Pk, MD 20742 USA. [Quayle, Brad] US Forest Serv, USDA, Remote Sensing Applicat Ctr, Salt Lake City, UT USA. [Lorenz, Eckehard] German Aerosp Ctr, Inst Opt Sensor Syst, Berlin, Germany. [Morelli, Fabiano] Brazilian Inst Space Res, Sao Jose Dos Campos, Brazil. RP Schroeder, W (reprint author), Univ Maryland, Dept Geog Sci, 2181 LeFrak Hall, College Pk, MD 20742 USA. EM wschroed@umd.edu RI Schroeder, Wilfrid/F-6738-2010 FU NASA Applied Sciences Program [NNX12AQ87G]; USDA Forest Service Remote Sensing Applications Center; GOES-R Algorithm Working Group at NOAA's National Environmental Satellite, Data, and Information Service FX We thank DLR, Erik Borg, and Doris Klein for hosting and sharing data from the small biomass burning experiment in Demmin/Germany on 17 August 2013. We also thank INPE for hosting the small biomass burning experiment on 19 January 2015 in Cachoeira Paulista/Brazil, and Andre Lima for sharing field data. Funding support for this study was provided by the NASA Applied Sciences Program (grant #NNX12AQ87G), the USDA Forest Service Remote Sensing Applications Center, and the GOES-R Algorithm Working Group at NOAA's National Environmental Satellite, Data, and Information Service. We thank four anonymous reviewers for their comments. NR 48 TC 2 Z9 2 U1 19 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 210 EP 220 DI 10.1016/j.rse.2015.08.032 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900018 ER PT J AU Wulder, MA White, JC Loveland, TR Woodcock, CE Belward, AS Cohen, WB Fosnight, EA Shaw, J Masek, JG Roy, DP AF Wulder, Michael A. White, Joanne C. Loveland, Thomas R. Woodcock, Curtis E. Belward, Alan S. Cohen, Warren B. Fosnight, Eugene A. Shaw, Jerad Masek, Jeffrey G. Roy, David P. TI The global Landsat archive: Status, consolidation, and direction SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat Global Archive Consolidation; LGAC; Satellite; Monitoring; Landsat ID TERM ACQUISITION PLAN; COVER; RESOLUTION; MISSION; IMAGERY; AVAILABILITY; CONTINUITY; SATELLITE; ACCESS; RECORD AB New and previously unimaginable Landsat applications have been fostered by a policy change in 2008 that made analysis-ready Landsat data free and open access. Since 1972, Landsat has been collecting images of the Earth, with the early years of the program constrained by onboard satellite and ground systems, as well as limitations across the range of required computing, networking, and storage capabilities. Rather than robust on-satellite storage for transmission via high bandwidth downlink to a centralized storage and distribution facility as with Landsat-8, a network of receiving stations, one operated by the U.S. government, the other operated by a community of International Cooperators (ICs), were utilized. ICs paid a fee for the right to receive and distribute Landsat data and over time, more Landsat data was held outside the archive of the United State Geological Survey (USGS) than was held inside, much of it unique. Recognizing the critical value of these data, the USGS began a Landsat Global Archive Consolidation (LGAC) initiative in 2010 to bring these data into a single, universally accessible, centralized global archive, housed at the Earth Resources Observation and Science (EROS) Center in Sioux Falls, South Dakota. The primary LGAC goals are to inventory the data held by ICs, acquire the data, and ingest and apply standard ground station processing to generate an LIT analysis-ready product. As of January 1, 2015 there were 5,532,454 images in the USGS archive. LGAC has contributed approximately 3.2 million of those images, more than doubling the original USGS archive holdings. Moreover, an additional 23 million images have been identified to date through the LGAC initiative and are in the process of being added to the archive. The impact of LGAC is significant and, in terms of images in the collection, analogous to that of having had two additional Landsat-5 missions. As a result of LGAC, there are regions of the globe that now have markedly improved Landsat data coverage, resulting in an enhanced capacity for mapping, monitoring change, and capturing historic conditions. Although future missions can be planned and implemented, the past cannot be revisited, undetscoring the value and enhanced significance of historical Landsat data and the LGAC initiative. The aim of this paper is to report the current status of the global USGS Landsat archive, document the existing and anticipated contributions of LGAC to the archive, and characterize the current acquisitions of Landsat-7 and Landsat-8. Landsat-8 is adding data to the archive at an unprecedented rate as nearly all terrestrial images are now collected. We also offer key lessons learned so far from the LGAC initiative, plus insights regarding other critical elements of the Landsat program looking forward, such as acquisition, continuity, temporal revisit, and the importance of continuing to operationalize the Landsat program. Crown Copyright (C) 2015 Published by Elsevier Inc All rights reserved. C1 [Wulder, Michael A.; White, Joanne C.] Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. [Loveland, Thomas R.; Fosnight, Eugene A.] US Geol Survey, Earth Resources Observat & Sci EROS Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. [Woodcock, Curtis E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Belward, Alan S.] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, I-20133 Milan, VA, Italy. [Cohen, Warren B.] US Forest Serv, Forestry Sci Lab, USDA, Corvallis, OR 97331 USA. [Shaw, Jerad] Stinger Ghaffarian Technol, Earth Resources Observat & Sci EROS Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. [Masek, Jeffrey G.] NASA, Biospher Sci Lab, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Roy, David P.] South Dakota State Univ, Geospatial Sci Ctr Excellence, Brookings, SD 57007 USA. RP Wulder, MA (reprint author), Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. EM mike.wulder@canada.ca OI White, Joanne/0000-0003-4674-0373; Wulder, Michael/0000-0002-6942-1896 FU Canadian Space Agency (CSA) Government Related Initiatives Program (GRIP); Canadian Forest Service (CFS) of Natural Resources Canada FX This communication has been made possible and enriched by the ongoing discussions and deliberations of the USGS/NASA Landsat Science Team (https://landsat.usgs.gov/science_Landsat_Science_Team.php). The participation of Wulder and White was supported as part of the "National Terrestrial Ecosystem Monitoring System (NTEMS): Timely and detailed national cross-sector monitoring for Canada" project jointly funded by the Canadian Space Agency (CSA) Government Related Initiatives Program (GRIP) and the Canadian Forest Service (CFS) of Natural Resources Canada. We greatly appreciate the time committed and the insightful comments made by three anonymous reviewers that helped us to improve the manuscript. NR 46 TC 11 Z9 11 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD NOV PY 2016 VL 185 SI SI BP 271 EP 283 DI 10.1016/j.rse.2015.11.032 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EA1AY UT WOS:000386321900023 ER PT J AU Rodrigues, WP Machado, JA da Silva, JR Figueiredo, FAMMD Ferraz, TM Ferreira, LS Bezerra, LBD de Abreu, DP Bernado, WD Passos, LC de Sousa, EF Glenn, DM Ramalho, JC Campostrini, E AF Rodrigues, Weverton Pereira Machado Filho, Jose Altino da Silva, Jefferson Rangel Moura de Assis Figueiredo, Fabio Afonso Mazzei Ferraz, Tiago Massi Ferreira, Luciene Souza da Silva Bezerra, Luan Baritiello de Abreu, Deivisson Pelegrino Bernado, Wallace de Paula Passos, Leticia Cespom de Sousa, Elias Fernandes Glenn, David Michael Ramalho, Jose Cochicho Campostrini, Eliemar TI Whole-canopy gas exchanges in Coffea sp is affected by supra-optimal temperature and light distribution within the canopy: The insights from an improved multi-chamber system SO SCIENTIA HORTICULTURAE LA English DT Article DE Climate change; Heat stress; Photosynthesis; Whole plant; Coffee tree ID CLIMATE-CHANGE; STOMATAL CONDUCTANCE; NET PHOTOSYNTHESIS; PLANT RESPIRATION; PARTICLE FILMS; ARABICA; LEAF; CO2; YIELD; FIELD AB Whole-canopy gas exchange provides a more accurate measurement of CO2 assimilation (A(c)) and transpiration (E-c) compared to single-leaf. The objectives of this study were to evaluate whole-canopy gas exchange of 2 coffee species: Coffea arabica cv. Catuai Amarelo-Catuai and Coffea canephora cv. Emcapa 8111 Clone 02-Clone 02 during two different seasons varying in temperature. Six plants >1-year-old of each species were grown in pots (100L) in a greenhouse. Soil moisture was maintained at field capacity. Data were continuously collected for 10 days during spring (September 2014 moderate temperatures) and summer (February 2015 with high temperature) and micrometeorological variables were monitored inside the greenhouse. Catuai had a lower area leaf and higher branch angle resulting in greater light distribution within the canopy that contributed to higher net photosynthesis and transpiration as compared to Clone 02 in both seasons. Catuai had reduced whole-canopy CO2 assimilation and transpiration during summer, mainly linked to reduced whole-canopy conductance. However, Clone 02 had similar whole-canopy CO2 assimilation and transpiration values in both seasons. Despite reduced gas exchange for Catuali during summer, the relationship between A(c) and E-c indicated that Catual had a greater instantaneous WUEC than Clone 02 during spring and there were no differences between species during summer. The dense canopy architecture of Clone 02 limited whole-canopy gas exchange due to poor light distribution within the canopy whereas Catual had better canopy light distribution resulting in higher photosynthetic rates than Clone 02 during spring. Additionally, the architecture had a negative effect when Catual experiences increased temperature that reduced whole-canopy conductance. High temperatures may limit the growth and production of coffee plants in tropical areas. (C) 2016 Elsevier B.V. All rights reserved. C1 [Rodrigues, Weverton Pereira; Machado Filho, Jose Altino; da Silva, Jefferson Rangel; Ferreira, Luciene Souza; da Silva Bezerra, Luan Baritiello; de Abreu, Deivisson Pelegrino; Bernado, Wallace de Paula; Passos, Leticia Cespom; de Sousa, Elias Fernandes; Campostrini, Eliemar] UENF, Ctr Ciencias & Tecnol Agr, Setor Fisiol Vegetal, BR-28013602 Darcy Ribeiro, RJ, Brazil. [Machado Filho, Jose Altino] Inst Capixaba Pesquisa Assistencia Tecn & Extensa, BR-29052010 Vitoria, ES, Brazil. [Moura de Assis Figueiredo, Fabio Afonso Mazzei; Ferraz, Tiago Massi] Univ Estadual Maranhao, Ctr Estudos Super Imperatriz, Rua GodofredoViana 1300, BR-65901480 Imperatriz, MA, Brazil. [Glenn, David Michael] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Ramalho, Jose Cochicho] ULisboa, Grp Interacoes Planta Ambiente & Biodiversidade P, Dept Recursos Nat Ambiente & Terr DRAT, Linking Landscape Environm Agr & Food LEAF,ISA, Ave Replibl, P-2784505 Oeiras, Portugal. [Ramalho, Jose Cochicho] UNL, FCT, GeoBioTec, P-2829516 Caparica, Portugal. RP Campostrini, E (reprint author), UENF, Ctr Ciencias & Tecnol Agr, Setor Fisiol Vegetal, BR-28013602 Darcy Ribeiro, RJ, Brazil. EM campostenator@gmail.com RI Rodrigues, Weverton/H-4296-2016; OI Rodrigues, Weverton/0000-0002-9137-474X; Campostrini, Eliemar/0000-0002-1329-1084; Ramalho, Jose/0000-0002-7639-7214 FU Fundacao Carlos Chagas de Apoio a Pesquisa do Estado do Rio de Janeiro (FAPERJ); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX The authors wish to thank Dr. F.M. DaMatta for helpful discussions and critical reading of the manuscript. Funding for this research has been provided through grants from Fundacao Carlos Chagas de Apoio a Pesquisa do Estado do Rio de Janeiro (FAPERJ) to W.P.R. Financial support has been provided from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). NR 55 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD NOV 1 PY 2016 VL 211 BP 194 EP 202 DI 10.1016/j.scienta.2016.08.022 PG 9 WC Horticulture SC Agriculture GA EA2GC UT WOS:000386409800024 ER PT J AU Negron, JF McMillin, J Sieg, CH Fowler, JF Allen, KK Wadleigh, LL Anhold, JA Gibson, KE AF Negron, Jose F. McMillin, Joel Sieg, Carolyn H. Fowler, James F. Allen, Kurt K. Wadleigh, Linda L. Anhold, John A. Gibson, Ken E. TI Variables associated with the occurrence of Ips beetles, red turpentine beetle and wood borers in live and dead ponderosa pines with post-fire injury SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Dendroctonus valens; fire injury; Ips beetles; Ips spp.; Pinus ponderosa; wood borers ID FUEL-REDUCTION TREATMENTS; MORTALITY FOLLOWING FIRE; TREE MORTALITY; BARK BEETLES; NORTHERN ARIZONA; PRESCRIBED FIRE; SIERRA-NEVADA; CONIFER MORTALITY; PINUS-PONDEROSA; EASTERN OREGON AB 1 Recently, wildfires and prescribed burning have become more frequent in conifer forests of western North America. 2 Most studies examining the impacts of insects on trees with post-fire injury have focused on contributions to tree mortality. Few studies have examined fire-caused injuries to estimate the probability of attack by insects. Scant data quantifying insect associations with one another, or with live and dead fire-injured trees, are available. 3 We examined live and dead trees with varying levels of fire injury in wildfires in Colorado, Montana, Arizona and the Black Hills aiming to determine fire injury associated with insect infestation, co-occurrence between insects and insect association with live and dead fire-injured trees. 4 Bole scorch height estimated the likelihood of attack by Ips spp. Diameter at breast height, bole scorch height and crown scorch height estimated the likelihood of attack by Dendroctonus valens LeConte. Diameter at breast height and bole scorch height estimated the likelihood of attack by wood borers. 5 Ips spp., Dendroctonus valens and wood borers were associated with one another. Ips spp. beetles and wood borers were associated with dead fire-injured trees, whereas D. valens was often associated with live fire-injured trees. 6 Focusing on certain fire-caused injuries may identify trees targeted by Ips spp. beetles, Dendroctonus valens and wood borers. C1 [Negron, Jose F.] US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect, Ft Collins, CO 80526 USA. [McMillin, Joel] US Forest Serv, USDA, Forest Hlth Protect, Reg 4,1249 South Vinnell Way,Suite 200, Boise, ID 83709 USA. [Sieg, Carolyn H.; Fowler, James F.] US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Allen, Kurt K.] US Forest Serv, USDA, Forest Hlth Protect, Reg 2,8221 South Highway 16, Rapid City, SD 57702 USA. [Wadleigh, Linda L.] US Forest Serv, USDA, Fire Management, Reg 3,1824 South Thompson St, Flagstaff, AZ 86001 USA. [Anhold, John A.] US Forest Serv, USDA, Forest Hlth Protect, Reg 3,2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Gibson, Ken E.] US Forest Serv, USDA, Forest Hlth Protect, Reg 1,200 East Broadway, Missoula, MT 59801 USA. RP Negron, JF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect, Ft Collins, CO 80526 USA. EM jnegron@fs.fed.us FU USDA FX Funding for this research was provided by USDA. Forest Service, Forest Health Protection, Special Technology Development Program, Project R2-2001-01, Region 1 Forest Health Protection, Region 2 Forest Health Management, Region 3 Forest Health Protection and the Rocky Mountain Research Station. We are grateful to Chad Hoffman, Noah Barstatis, Amy Uhlenhopp, John Popp, Dan Long, Denise Hardesty, Kathy Sullivan, Cassie McCraw, Laura Kaye, Matt Jedra, Brian Howell, Steve McKelvey, Kelly Williams, Joleen Atencio, Stacy Marlatt, Jason Watkins and Kelly Mogab for assisting with the data collection and entry. We thank Dave Turner, Laurie Porth and Scott Baggett for statistical support. The thoughtful reviews by three anonymous reviewers substantially improved previous versions of the manuscript. NR 70 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 313 EP 326 DI 10.1111/afe.12163 PG 14 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600001 ER PT J AU Haavik, LJ Dodds, KJ Ryan, K Allison, JD AF Haavik, Laurel J. Dodds, Kevin J. Ryan, Kathleen Allison, Jeremy D. TI Evidence that the availability of suitable pine limits non-native Sirex noctilio in Ontario SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Bottom-up effects; invasive species; natural enemies; population dynamics; top-down effects; wood borer ID BORER COLEOPTERA BUPRESTIDAE; BRONZE BIRCH BORER; WOODWASPS HYMENOPTERA-SIRICIDAE; NORTHEASTERN UNITED-STATES; BIOLOGICAL-CONTROL; NORTH-AMERICA; BEETLE COLEOPTERA; NATURAL ENEMIES; ECOLOGY; MANAGEMENT AB 1 Natural controls may not be effective in limiting non-native species that invade a new environment. Alternatively, factors present in the new environment can prevent an invader from becoming a serious pest. 2 We investigated whether existing natural enemies or the availability of susceptible and suitable host plants limited an established, non-native species Sirex noctilio F. (Siricidae: Hymenoptera). 3 Using several pine forests throughout Ontario, we examined the relationships between parasitism and S. noctilio activity, as well as S. noctilio activity and the availability of susceptible and suitable pine. We also evaluated parasitism at two points in time (5-7 years apart). 4 We found correlative evidence indicating that the availability of suitable hosts limited the S. noctilio population in Ontario. Sirex noctilio killed more pine when more pine was available, especially suppressed (highly suitable) pine. 5 More co-dominant (healthy) pines were attacked but not killed by S. noctilio at sites with more S. noctilio activity, indicating that higher densities of S. noctilio were needed to overwhelm healthy pines. 6 The role of parasitoids was less clear. Parasitism did not vary consistently with the amount of S. noctilio activity at sites. The two groups of parasitoids, Rhyssa and Ibalia, likely responded differently to presence of S. noctilio. C1 [Haavik, Laurel J.; Allison, Jeremy D.] Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada. [Dodds, Kevin J.] US Forest Serv, USDA, Forest Hlth Protect, 271 Mast Rd, Durham, NH 03824 USA. [Ryan, Kathleen; Allison, Jeremy D.] Univ Toronto, Fac Forestry, 33 Willcocks St, Toronto, ON M5S 3B3, Canada. [Haavik, Laurel J.] Univ Kansas, Dept Ecol & Evolutionary Biol, 1200 Sunnyside Ave, Lawrence, KS 66046 USA. [Ryan, Kathleen] Silv Econ Ltd, 913 Southwind Court, Newmarket, ON L3Y 6J1, Canada. RP Haavik, LJ (reprint author), Nat Resources Canada Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON P6A 2E5, Canada.; Haavik, LJ (reprint author), Univ Kansas, Dept Ecol & Evolutionary Biol, 1200 Sunnyside Ave, Lawrence, KS 66046 USA. EM ljhaavik@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 FU Natural Resources Canada; Ontario Ministry of Natural Resources; USDA Forest Service-Forest Health Protection FX We thank Ronald Fournier for producing Fig. 1; Sarah Drabble, Reg Nott, Nick Boyonoski, Sarah Crispell, Katelyn Kotska, Chanel Fall, Cydney Mihell, Brett Marshall, Shannon Escasa and Elder Batista for field and laboratory assistance; Al Foley for access to the Ontario Tree Seed Facility; and four anonymous reviewers for their helpful comments on the manuscript. This work was funded in part by Natural Resources Canada, The Ontario Ministry of Natural Resources and the USDA Forest Service-Forest Health Protection. NR 60 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 357 EP 366 DI 10.1111/afe.12167 PG 10 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600005 ER PT J AU Lopez-Martinez, V Sanchez-Martinez, G Jimenez-Garcia, D Perez-De la O, NB Coleman, TW AF Lopez-Martinez, Victor Sanchez-Martinez, Guillermo Jimenez-Garcia, Daniel Perez-De la O, Nidia B. Coleman, Tom W. TI Environmental suitability for Agrilus auroguttatus (Coleoptera: Buprestidae) in Mexico using MaxEnt and database records of four Quercus (Fagaceae) species SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Ecological niche; exotic wood borer; goldspotted oak borer; Mexican biogeographical provinces ID GOLDSPOTTED OAK BORER; SOUTHERN CALIFORNIA; FLORISTIC COMPOSITION; COMMUNITY STRUCTURE; COXALIS WATERHOUSE; DISTRIBUTIONS; WOODLANDS; PERFORMANCE; AGRIFOLIA; FORESTS AB 1 The goldspotted oak borer Agrilus auroguttatus Schaeffer is an invasive pest of three Quercus species in southern California, U.S.A. As a native and potentially indigenous exotic species, its distribution and potential damage is largely unknown in Mexico. 2 We used the maximum entropy algorithm to determine the bioclimatic variables that may explain the distribution of A. auroguttatus, as well as its interaction with the distribution of known hosts in Mexico. 3 Our model calculated high suitability for A. auroguttatus in the biogeographical provinces of California, the northern and southern areas of Sierra Madre Occidental and the northwestern area of the Mexican Plateau; moderate suitability was projected in the Baja California, Sonora and Sierra Madre Occidental provinces. Potential distribution of A. auroguttatus was overlapping with native ranges of Quercus agrifolia, Quercus emoryi Torrey and Quercus hypoleucoides A. Camus. Precipitation of the coldest quarter (23.6%), isothermality (23.5%), precipitation seasonality (17.3%) and mean temperature of the coldest quarter (11.2%) contributed most to explaining its distribution in Mexico. 4 The geographical interaction (i.e. the likelihood that the habitat would be suitable for coexistence) of A. auroguttatus with its hosts in Mexico was projected in the California province (46 382.60 km(2), probably as an exotic species) and the northwestern areas of Mexican Plateau and Sierra Madre Occidental provinces (74 013.45 km(2), probably as native). 5 Determining the areas of environmental suitability for A. auroguttatus and its host distribution can focus ground surveys, contribute to management efforts and assist in the development of risk assessments for Mexico. C1 [Lopez-Martinez, Victor; Perez-De la O, Nidia B.] Univ Autonoma Estado Morelos, Fac Ciencias Agr, Ave Chamilpa 1001, Cuernavaca 62209, Morelos, Mexico. [Sanchez-Martinez, Guillermo] INIFAP CIRNOC Campo Expt Pabellon, Programa Invest Sanidad Forestal & Agr, Km 32-5 Ctra Aguascalientes Zacatecas, Pabellon De Arteaga 20670, Mexico. [Jimenez-Garcia, Daniel] Benemerita Univ Autonoma Puebla, Inst Ciencias, Ctr Agroecol & Ambiente, 12 Sul 6301, Col San Manuel 72570, Puebla, Mexico. [Coleman, Tom W.] US Forest Serv, USDA, Forest Hlth Protect, 602 S Tippecanoe Ave, San Bernardino, CA 92408 USA. RP Lopez-Martinez, V (reprint author), Univ Autonoma Estado Morelos, Fac Ciencias Agr, Ave Chamilpa 1001, Cuernavaca 62209, Morelos, Mexico. EM victor.lopez@uaem.mx FU Programa de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIES); Programa para el Desarrollo Profesional Docente en Educacion Superior [DSA/103.5/16/2609]; CONACYT [260517]; USDA Forest Service, Forest Health Protection, Pacific Southwest Region FX This work was partially supported by the Programa de Fortalecimiento de la Calidad en Instituciones Educativas (PROFOCIES 2014) and Programa para el Desarrollo Profesional Docente en Educacion Superior (DSA/103.5/16/2609). Nidia Belgica Perez-De la O received a graduate grant (CONACYT No. 260517). T. W. Coleman was supported by USDA Forest Service, Forest Health Protection, Pacific Southwest Region. NR 73 TC 0 Z9 0 U1 21 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD NOV PY 2016 VL 18 IS 4 BP 409 EP 418 DI 10.1111/afe.12174 PG 10 WC Entomology SC Entomology GA DZ1SI UT WOS:000385619600010 ER PT J AU Hergert, GW Margheim, JF Pavlista, AD Martin, DL Isbell, TA Supalla, RJ AF Hergert, G. W. Margheim, J. F. Pavlista, A. D. Martin, D. L. Isbell, T. A. Supalla, R. J. TI Irrigation response and water productivity of deficit to fully irrigated spring camelina SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Biodiesel; Limited irrigation; High plains aquifer; Ground; Water decline ID LIMITED IRRIGATION; CROPPING SYSTEMS; WESTERN NEBRASKA; OILSEED CROPS; HIGH-PLAINS; SATIVA OIL; CANOLA; GROWTH; YIELD; FUEL AB Camelina [Camelina sativa L Crantz] is an oil seed crop that could be adapted to the northern High Plains of the USA as a biofuel crop. Decreased ground water allocations in Nebraska necessitated determining the impact of limited irrigation on camelina. The objective of this research was to determine the effects of a range of irrigation levels on camelina yield, oil content, soil water changes and water productivity. The study was conducted for four growing seasons at two locations in western Nebraska. One site had a sandy soil whereas the other was a silt loam. Camelina was planted in early to mid-April. Cumulative irrigation treatments were 0, 100, 200, and 300 mm with the highest rate adjusted to be non-ET limiting. Camelina extracted soil water from 0.9 to 1 m depths which was shallower than canola. It showed significant response to irrigation during dry years but no response in above-average precipitation years. A water use efficiency of 7.0 kg mm(-1) with 125 mm ETc required to produce the first unit of seed yield was shown. Camelina seed yield ranged from 428 to 2867 kg ha(-1) with 187 and 536 mm of cumulative ET. In 2007 and 2008 camelina exhibited peak values in water consumed at 9-10 weeks after planting. Deficit irrigation reduced ET and yield plus accelerated maturity in those years. Oil content was increased by irrigation during drier years with no effect with high growing season precipitation. Oil content ranged from 25 to 50% depending on year, irrigation level and disease. Downey mildew significantly reduced oil content during 2009. Deficit irrigated camelina could be an alternative biofuel crop for this region but further genetic improvement would enhance its competiveness with spring canola. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hergert, G. W.; Margheim, J. F.; Pavlista, A. D.] Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. [Martin, D. L.; Supalla, R. J.] Univ Nebraska, Lincoln, NE 68583 USA. [Isbell, T. A.] USDA ARS, Peoria, IL 61604 USA. RP Hergert, GW (reprint author), Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. EM ghergert1@unl.edu OI Hergert, Gary/0000-0001-6944-6199 FU USDA Risk Management Agency FX Funding for this project was provided by the USDA Risk Management Agency under several contracts. Without their support we could not have completed this research. NR 50 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 46 EP 53 DI 10.1016/j.agwat.2016.06.009 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900005 ER PT J AU Moriasi, DN King, KW Bosch, DD Bjorneberg, DL Teet, S Guzman, JA Williams, MR AF Moriasi, Daniel N. King, Kevin W. Bosch, David D. Bjorneberg, Dave L. Teet, Stephen Guzman, Jorge A. Williams, Mark R. TI Framework to parameterize and validate APEX to support deployment of the nutrient tracking tool SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Agricultural policy environmental eXtender (APEX); Nutrient tracking tool (NTT); Sensitivity analysis; Uncertainty analysis; Water quality; Water quantity ID WATER-QUALITY MODELS; AGRICULTURAL POLICY/ENVIRONMENTAL EXTENDER; MANAGEMENT STRATEGIES; HYDROLOGIC-MODELS; CALIBRATION; UNCERTAINTY; SENSITIVITY; PRODUCTIVITY; SIMULATION; GUIDELINES AB The Agricultural Policy Environmental eXtender (APEX) model is the scientific basis for the Nutrient Tracking Tool (Nu). NTT is an enhanced version of the Nitrogen Trading Tool, a user-friendly web-based computer program originally developed by the USDA. NTT was developed to estimate reductions in nutrient losses to the environment associated with alternative practices. The relatively easy access and ease with which the interface can be used has provided opportunities to demonstrate NTT in locations throughout the country; however, the absence of a clearly defined, consistent approach to parameterization and validation has raised questions over the reliability and consistency of simulated results. In this study: guidelines for parameterization and validation of APEX were developed based on literature review findings and the authors' experience; and a case study was provided to illustrate how the developed guidelines are applied. The developed guidelines are in the form of recommendations covering essential phases of model simulation studies as well as a clear interpretation of model performance evaluation criteria thresholds and model simulation performance results. These guidelines were successfully applied in the central Ohio case study. The most sensitive water yield parameters and their respective reasonable range of values were determined. Simulated monthly and annual water yield values were within 5% and 15% of observed values during the calibration and validation periods, respectively. Overall, the developed guidelines together with the illustrative case study example are intended to serve as the framework to parameterize and validate APEX to support nation-wide deployment of NIT. This framework can be easily modified and used in additional APEX and other modeling studies. Published by Elsevier B.V. C1 [Moriasi, Daniel N.; Teet, Stephen] USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. [King, Kevin W.; Williams, Mark R.] USDA ARS, Soil Drainage Res Lab, Columbus, OH 43210 USA. [Bosch, David D.] USDA ARS, Southeast Watershed Res, Tifton, GA 31793 USA. [Bjorneberg, Dave L.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Guzman, Jorge A.] Waterborne Environm Inc, Champaign, IL 61820 USA. RP Moriasi, DN (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM daniel.moriasi@ars.usda.gov FU USDA-OEM FX Funding for this study was provided by USDA-OEM. NR 57 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 146 EP 164 DI 10.1016/j.agwat.2016.07.009 PG 19 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900014 ER PT J AU Anapalli, SS Ahuja, LR Gowda, PH Ma, LW Marek, G Evett, SR Howell, TA AF Anapalli, Saseendran S. Ahuja, Lajpat R. Gowda, Prasanna H. Ma, Liwang Marek, Gary Evett, Steven R. Howell, Terry A. TI Simulation of crop evapotranspiration and crop coefficients with data in weighing lysimeters SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Evapotranspiration; Crop coefficient; Lysimeter; Agricultural system model; Irrigation; Water management ID CENTRAL GREAT-PLAINS; CLIMATE-CHANGE; MODEL; WATER; IRRIGATION; RZWQM2; AGRICULTURE; ACCURACY; SYSTEM; MAIZE AB Accurate quantification of crop evapotranspiration (ET) is critical to optimizing irrigation water productivity, especially, in the semiarid regions of the world where limited rainfall is supplemented by irrigation for crop production. In this context, cropping system models are potential tools for predicting ET or crop water requirements in agriculture across soils and climates and assist in developing decision support tools for irrigation. The objective of this study was to evaluate the accuracy of RZWQM2 simulated ET for fully irrigated silage (2006 and 2007) and grain corn (1990) against measured crop water use and soil evaporation with large weighing lysimeters in the Texas High Plains. An extended Shuttleworth and Wallace method was used to estimate potential crop ET (E and T) demand in RZWQM2. The Nimah and Hanks approach was used for crop water uptake and Richard's Equation for soil water redistribution modeling. Simulations of biomass, leaf area index, soil water storage, and ET were reasonably close to the measured data. Root Mean Squared Deviation (RMSD) for corn biomass was between 1 and 2.1 MT ha(-1), LAI between 0.33 and 0.88, water in the soil between 2 and 2.9 cm for a 190 cm soil profile, and actual daily crop ET between 1.0 to 1.5 mm across the three years of measured data. Arithmetic mean deviation (MD) for ET ranged from -0.10 to 0.40 mm. Fallow soil evaporation before and after corn planting was simulated within MD of -0.03-0.003 mm. The crop coefficients (Kc) calculated with measured ET and the short grass or alfalfa crop reference ET methods varied from year to year. The Kc values obtained by using the simulated ET and alfalfa reference ET were close to Kc values using measured ET, within RMSD of 0.17, and could be used to obtain long-term average Kc values for scheduling irrigation. Published by Elsevier B.V. C1 [Anapalli, Saseendran S.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Ahuja, Lajpat R.; Ma, Liwang] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80522 USA. [Gowda, Prasanna H.] USDA ARS, Forage & Livestock Prod Res Unit, El Reno, OK USA. [Marek, Gary; Evett, Steven R.] USDA ARS, CPRL, 2300 Expt Stn Rd, Bushland, TX 79012 USA. [Howell, Terry A.] USDA ARS, POB 5649, Lago Vista, TX USA. RP Anapalli, SS (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM Saseendransa@hotmail.com FU USDA-Agricultural Research Service, Kansas State University; Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 43 TC 0 Z9 0 U1 19 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 274 EP 283 DI 10.1016/j.agwat.2016.08.009 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900026 ER PT J AU Zhang, BB Feng, G Read, JJ Kong, XB Ouyang, Y Adeli, A Jenkins, JN AF Zhang, Bangbang Feng, Gary Read, John J. Kong, Xiangbin Ouyang, Ying Adeli, Ardeshir Jenkins, Johnie N. TI Simulating soybean productivity under rainfed conditions for major soil types using APEX model in East Central Mississippi SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Simulating; APEX model; Soybean productivity; Rainfed condition; Yield difference; Irrigation management ID CROP PRODUCTION; YIELD GAPS; ZEA-MAYS; WATER; IRRIGATION; MAIZE; MANAGEMENT; DRY; WHEAT; WET AB Knowledge of soybean yield constraints under rainfed conditions on major soil types in East Central Mississippi would assist growers in the region to effectively utilize the benefits of water/irrigation management. The objectives of this study were to use the Agricultural Policy/Environmental eXtender (APEX) agro-ecosystem model to simulate rainfed soybean grain yield (GY) for nine major soils during 14 years (2002-2015) and then to evaluate selected model inputs/outputs in relation to irrigation management that may decrease difference in simulated GY among the different soils. Values for GY ranged broadly from 2.24 to 6.14 Mg ha(-1) across soils and years, giving a maximum yield difference of 3.90 Mg ha(-1). For the average GY of nine soils, the range was from 3.52 to 5.42 Mg ha(-1) over 14 years. Averaged across 14 years, GY ranged from 3.66 to 4.90 Mg ha(-1) across the nine soils and was affected by difference in soil texture (clay and sand percentages) and soil available water content (AWC). Simulations revealed relatively high water stress during the R4, R5 and R6 stages of plant development (early-to mid-fruit development), suggesting great potential to enhance soybean yield if some irrigation is provided during these critical water stress periods; whereas, the potential was accordingly less in the normal and wet years. Results indicated installing irrigation on Griffith, Sumter or Demopolis soils would have a large impact through increased crop productivity and yield stability. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhang, Bangbang; Kong, Xiangbin] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. [Zhang, Bangbang; Kong, Xiangbin] Natl Minist Land & Resources, Lab Farmland Qual Monitoring & Control, Beijing 100193, Peoples R China. [Feng, Gary; Read, John J.; Adeli, Ardeshir; Jenkins, Johnie N.] ARS, Genet & Sustainable Agr Res Unit, USDA, Starkville, MS 39762 USA. [Ouyang, Ying] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Starkville, MS 39762 USA. RP Kong, XB (reprint author), China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. EM kxb@cau.edu.cn FU National Social Science Foundation of China [14AZD031]; Beijing Natural Science Foundation, China [8151001]; Missisippi Soybean Promotion Board [62-2015]; China Scholarship Council [201406350040] FX This work was completed in Genetics and Sustainable Agriculture Research Unit, USDA-ARs as a visiting Ph.D candidate sponsered by China Scholarship Council (No. 201406350040). This work was supported by National Social Science Foundation of China (Grant No. 14AZD031), Beijing Natural Science Foundation, China (Grant No. 8151001), and Missisippi Soybean Promotion Board (62-2015). We especially thank Dr. Luca Doro for his help in APEX model application and Dr. Zhen Sui for her assistance in statistical analysis. NR 54 TC 1 Z9 1 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 379 EP 391 DI 10.1016/j.agwat.2016.08.022 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900037 ER PT J AU Han, M Zhang, HH DeJonge, KC Comas, LH Trout, TJ AF Han, Ming Zhang, Huihui DeJonge, Kendall C. Comas, Louise H. Trout, Thomas J. TI Estimating maize water stress by standard deviation of canopy temperature in thermal imagery SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Maize; Water stress; Canopy temperature standard deviation; Thermal imagery; Deficit irrigation ID INFRARED THERMOMETRY; LEAF TEMPERATURES; INDEX; IRRIGATION; INDICATOR; TRANSPIRATION; VARIABILITY; RADIATION; TREES; CORN AB A new crop water stress indicator, standard deviation of canopy temperature within a thermal image (CTSD), was developed to monitor crop water status. In this study, thermal imagery was taken from maize (Zea mays L.) under various levels of deficit irrigation at different crop growing stages. The Expectation Maximization algorithm was used to estimate the canopy temperature distribution from thermal imagery under a range of crop coverage and water stress conditions. Soil water deficit (SWD), leaf water potential (psi), stomatal conductance, and other crop water stress indices were used to evaluate the CTSD. We found that the temperature differences between sunlit and shaded parts of the canopy would increase with larger canopy resistance in the sunlit part of the crop canopy. The CTSD well described impact of irrigation events (timing and depth) on crop water stress. All water stress measurements showed statistically significant relationship with CTSD. Although CTSD is not sensitive to small changes in water stress, the result suggests that the canopy temperature standard deviation could be used as a water stress indicator. This index has strong application potential because it only relies on the canopy temperature itself, and is easy to calculate. Moreover, it may also be applied to high resolution thermal imagery from other remote sensing platforms, such as unmanned aerial vehicles. Published by Elsevier B.V. C1 [Han, Ming; Zhang, Huihui; DeJonge, Kendall C.; Comas, Louise H.; Trout, Thomas J.] USDA ARS, Water Management & Syst Res Unit, 2150 Ctr Ave,Bldg D,Suite 320, Ft Collins, CO 80526 USA. [Han, Ming] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80526 USA. RP Zhang, HH (reprint author), USDA ARS, Water Management & Syst Res Unit, 2150 Ctr Ave,Bldg D,Suite 320, Ft Collins, CO 80526 USA. EM Huihui.Zhang@ars.usda.gov OI Zhang, Huihui/0000-0002-9781-6086; Trout, Thomas/0000-0003-1896-9170 NR 32 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 400 EP 409 DI 10.1016/j.agwat.2016.08.031 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900039 ER PT J AU Zhang, BB Feng, G Kong, XB Lal, R Ouyang, Y Adeli, A Jenkins, JN AF Zhang, Bangbang Feng, Gary Kong, Xiangbin Lal, Rattan Ouyang, Ying Adeli, Ardeshir Jenkins, Johnie N. TI Simulating yield potential by irrigation and yield gap of rainfed soybean using APEX model in a humid region SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Soybean; Yield potential; Yield gap; Irrigation timing and amount; Irrigation cost-return analysis; APEX model ID WATER-USE EFFICIENCY; US CORN-BELT; CROP YIELD; DEFICIT IRRIGATION; PLANTING DATE; SEED YIELD; CLAY SOIL; MANAGEMENT; EVAPORATION; GROWTH AB Soybean is generally grown under rainfed conditions in a humid region, Mississippi, USA. In order to determine how much maximum yield could be increased by irrigation, it is crucial to investigate the yield potential (Y-p) without any drought stress and yield gap (Y-g, between Y-p and rainfed yield (Y-w)). Further, it is also important to determine the amount of irrigation water needed to alleviate any drought stress during the entire growing season, and conduct cost-return analysis for irrigated soybean. Therefore, the objectives of this study were to: (1) simulate Y-p, analyze Y-g and determine the irrigation timing and amount needed to achieve Y-p for soybean using the Agricultural Policy/Environmental eXtender (APEX) model; and (2) compute water use efficiency (WUE), irrigation water use efficiency (I-WUE) and conduct the cost-return analysis on irrigation events. Simulated Y-p of soybean without water stress for nine soil types from 2002 to 2014 ranged from 4.47 to 6.51 Mg ha(-1), and was strongly correlated with accumulative solar radiation during the growing season (R-2 = 0.71, P <= 0.01). The Y-g in dry years was much higher than that in normal and wet years, with average Y-g of 1.58, 0.60 and 0.71 Mg ha(-1) for dry, normal and wet years, respectively. Griffith, Sumter and Demopolis soils had the highest average Y-g over 13 years, ranging from 1.37 to 1.60 Mg ha(-1). The average irrigation amount was 308, 192 and 157 mm in dry, normal and wet years, respectively. The average irrigation amount was 75 mm from R1 to R8 stages. The WUE of nine soil types from 2002 to 2014, under non-limiting water conditions, ranged from 9.5 to 13.8 kg ha(-1) mm(-1). The magnitude of Y-g was the principle factor affecting I-WUE among nine soil types. The average I-WUE over 13 years ranged from 1.8 to 7.8 kg ha(-1) mm(-1) for nine soil types. Compared with a rainfed condition, average net return of irrigated soybean increased by 93 $ ha(-1) (dollar per hectare) among nine soil types from 2002 to 2014. The average net return increased by 195, 58 and 70 $ ha(-1) for dry, normal and wet years, respectively. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zhang, Bangbang; Kong, Xiangbin] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. [Zhang, Bangbang; Kong, Xiangbin] Natl Minist Land & Resources, Key Lab Farmland Qual Monitoring & Control, Beijing 100193, Peoples R China. [Feng, Gary; Adeli, Ardeshir; Jenkins, Johnie N.] ARS, Genet & Sustainable Agr Res Unit, USDA, Starkville, MS 39762 USA. [Lal, Rattan] Ohio State Univ, Sch Environm & Nat Resources, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [Ouyang, Ying] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Starkville, MS 39762 USA. RP Kong, XB (reprint author), China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. EM kxb@cau.edu.cn FU Chinese Scholar Council [201406350040]; National Social Science Foundation of China [14AZD031]; Natural Science Foundation of Beijing, China [8151001]; Mississippi Soybean Promotion Board [62-2015] FX This work was completed in Genetics and Sustainable Agriculture Research Unit, USDA-ARS as a visiting Ph.D candidate sponsored by Chinese Scholar Council (No. 201406350040). This work was supported by National Social Science Foundation of China (Grant No. 14AZD031), Natural Science Foundation of Beijing, China (Grant No. 8151001), and Mississippi Soybean Promotion Board (62-2015). We especially thank Luca Doro for his help in APEX model application and Joshua H. Holtcamp for his lab work measuring soil and crop parameters. NR 73 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD NOV PY 2016 VL 177 BP 440 EP 453 DI 10.1016/j.agwat.2016.08.029 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY7PY UT WOS:000385322900043 ER PT J AU Good, C Marancik, DP Welch, TJ May, T Davidson, J Summerfelt, S AF Good, Christopher Marancik, David P. Welch, Timothy J. May, Travis Davidson, John Summerfelt, Steven TI Systemic granuloma observed in Atlantic salmon Salmo salar raised to market size in a freshwater recirculation aquaculture system SO AQUACULTURE RESEARCH LA English DT Article DE Atlantic salmon; systemic granuloma; histopathology; recirculation aquaculture ID BLOOD-CHEMISTRY; RAINBOW-TROUT; FISH; AGENT C1 [Good, Christopher; May, Travis; Davidson, John; Summerfelt, Steven] Conservat Fund, Inst Freshwater, 1098 Turner Rd, Shepherdstown, WV 25443 USA. [Marancik, David P.; Welch, Timothy J.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. [Marancik, David P.] Fish Vet Grp, 350 Commercial St, Portland, ME 04101 USA. RP Good, C (reprint author), Conservat Fund, Inst Freshwater, 1098 Turner Rd, Shepherdstown, WV 25443 USA. EM c.good@freshwaterinstitute.org FU Gordon and Betty Moore Foundation; Atlantic Salmon Federation; USDA Agricultural Research Service (ARS) [59-1930-5-510] FX We are grateful to the Gordon and Betty Moore Foundation and the Atlantic Salmon Federation for providing funding for the overall salmon grow-out trial, as well as the USDA Agricultural Research Service (ARS) that supported this independent study under Agreement No. 59-1930-5-510. Our gratitude is extended to Dr Gregory Wiens of the USDA-ARS-NCCCWA for critical review of the manuscript. Appreciation is also extended to Ms Jennifer Lipscomb (USDA-ARS-NCCCWA) for conducting the molecular analyses. The authors do not have any conflicts of interest to declare. Experimental protocols described are in compliance with the Animal Welfare Act (9CFR), and were approved by our Institutional Animal Care and Use Committee (IACUC). Use of trade names does not imply endorsement by the US Government. NR 18 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD NOV PY 2016 VL 47 IS 11 BP 3679 EP 3683 DI 10.1111/are.12790 PG 5 WC Fisheries SC Fisheries GA DZ1SZ UT WOS:000385621700031 ER PT J AU Lin, WC Li, XJ Dai, ZK Zhang, XH Chang, S Zhao, P Zhang, HM Chen, F Xie, QM AF Lin, Wencheng Li, Xinjian Dai, Zhenkai Zhang, Xinheng Chang, Shuang Zhao, Peng Zhang, Huanmin Chen, Feng Xie, Qingmei TI Molecular epidemiology of J-subgroup avian leukosis virus isolated from meat-type chickens in southern China between 2013 and 2014 SO ARCHIVES OF VIROLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; COMPLETE PROVIRAL GENOME; SEQUENCE-ANALYSIS; UNITED-STATES; LAYER FLOCKS; HEMANGIOMA; FIELD; HPRS-103; ELEMENT; STRAIN AB Members of avian leukosis virus subgroup J (ALV-J) cause various diseases associated with tumor formation and decreased fertility, resulting in major economic losses in the poultry industry worldwide. To assess the status of ALV-J infection in meat-type chickens in southern China, the molecular epidemiology of ALV-J strains was investigated. A total of 265 clinical samples collected from southern China from 2013 to 2014 were investigated in this study for the presence of ALV-J, which resulted in 12 virus isolates. Phylogenetic analysis showed that 91.7 % (11/12) of the ALV-J isolates have possessed high homology to Chinese layer isolates and belong to one subgroup. One of the ALV isolates (designated GD1411-1) was relatively closely related to the ALV-J broiler isolates, indicating that the GD1411-1 isolate might be a transition strain. Several unique nucleotide substitutions in gp85 and the U3 region were detected in all 12 ALV-J isolates. This study provides some interesting information on the molecular characterization of ALV-J isolates. These findings will be beneficial for understanding of the pathogenic mechanism of ALV-J infection. C1 [Lin, Wencheng; Li, Xinjian; Dai, Zhenkai; Zhang, Xinheng; Chen, Feng; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Key Lab Chicken Genet Breeding & Reprod,Minist Ed, Guangzhou 510642, Guangdong, Peoples R China. [Chang, Shuang; Zhao, Peng] Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Lin, Wencheng; Chen, Feng; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Wencheng; Xie, Qingmei] Southe China Collaborat Innovat Ctr Poultry Dis C, Guangzhou 510642, Guangdong, Peoples R China. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Key Lab Chicken Genet Breeding & Reprod,Minist Ed, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Southe China Collaborat Innovat Ctr Poultry Dis C, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Natural Science Foundation of Guangdong Province [S2013030013313]; National Natural Science Foundation of China [31472217]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), National Natural Science Foundation of China (Grant No. 31472217) and Guangdong Province Science and Technology Plan Project (Grant Nos. 2012B020306002, 2012B091100078). NR 24 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD NOV PY 2016 VL 161 IS 11 BP 3039 EP 3046 DI 10.1007/s00705-016-3003-8 PG 8 WC Virology SC Virology GA DY6QF UT WOS:000385252100009 PM 27503348 ER PT J AU Seo, EY Lim, S Hammond, J Moon, JS Lim, HS AF Seo, Eun-Young Lim, Seungmo Hammond, John Moon, Jae Sun Lim, Hyoun-Sub TI Complete genome sequence of a novel potyvirus, callistephus mottle virus, identified in Callistephus chinensis SO ARCHIVES OF VIROLOGY LA English DT Article ID GENE AB The complete genomic sequence of a novel putative member of the genus Potyvirus was detected from Callistephus chinensis (China aster) in South Korea. The genomic RNA consists of 9,859 nucleotides (excluding the 3' poly(A) tail) and contains the typical open reading frame of potyviruses, encoding a putative large polyprotein of 3,154 amino acids. The Callistephus virus is most closely related to plum pox virus and members of the ApVY subgroup which showed 50-52 % polyprotein amino acid sequence identity. These results suggest that the Callistephus virus is a novel member of the genus Potyvirus, tentatively named "callistephus mottle virus" (CalMV). C1 [Seo, Eun-Young; Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Daejeon 305764, South Korea. [Lim, Seungmo; Moon, Jae Sun] Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 305806, South Korea. [Lim, Seungmo; Moon, Jae Sun] Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 305350, South Korea. [Hammond, John] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Daejeon 305764, South Korea.; Moon, JS (reprint author), Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 305806, South Korea.; Moon, JS (reprint author), Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 305350, South Korea. EM jsmoon@kribb.re.kr; hyounlim@cnu.ac.kr FU Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ011113] FX This research was supported by a Grant from the Next-Generation BioGreen 21 Program (PJ011113), Rural Development Administration, Republic of Korea. NR 10 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD NOV PY 2016 VL 161 IS 11 BP 3281 EP 3283 DI 10.1007/s00705-016-3001-x PG 3 WC Virology SC Virology GA DY6QF UT WOS:000385252100034 PM 27480416 ER PT J AU Pan, ZL Johnson, DA Wei, ZJ Ma, L Rong, YP AF Pan, Zhanlei Johnson, Douglas A. Wei, Zhijun Ma, Lei Rong, Yuping TI Non-growing season soil CO2 efflux patterns in five land-use types in northern China SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Grazed steppe; Leymus chinensis pasture; Annual cropland; Carbon dioxide; Inter-annual variability ID CARBON-DIOXIDE FLUXES; INTERANNUAL VARIABILITY; AGROPASTORAL REGION; INNER-MONGOLIA; RESPIRATION; GRASSLANDS; EXCHANGE; ECOSYSTEM; CLIMATE; STEPPE AB Heavy grazing and unsuitable farming practices have led to grassland degradation in northern China. This study examined soil CO2 efflux (Fc) from five land-use types during the non-growing season on the southeastern edge of the Mongolian Plateau in China. The land-use types included three native vegetation steppes subjected to differing stocking rates [ungrazed (UG), moderately grazed (MG) and heavily grazed (HG)], a fertilized annual cropland (CL) and a perennial pasture (PP) used for haying and winter grazing. Values of Fc were measured at 3-day to 2-week intervals during the non-growing season in two contrasting hydrological years (2012-13 and 2013-14) using closed chambers. The Fc during 1 Oct. 2013 to 30 April 2014 averaged 475 mg C m(-2) for all sites compared to a significantly (P < 0.05) lower Fc (102 mg C m(-2)) during 1 Oct. 2012 to 30 April 2013. The seasonal Fc patterns followed the same trend during the two non-growing seasons with greater Fc observed in the autumn and spring freeze-thaw periods compared to the winter permanently frozen period, which accounted for 4.8% of accumulated total non-growing season Fc. The heavily grazed site showed less soil CO2 efflux compared to UG, MG, PP and CL land-use types due to a larger reduction in gross primary productivity (GPP) compared to ecosystem respiration. Grazing reduced Fc by 23% for MG and 32% for HG compared to UG. Soil CO2 efflux from the PP land-use type, which was grazed during the non-growing season, was 23% greater than that from the UG and CL land-use types. Air temperature during the non-growing season was the main factor controlling soil CO2 efflux (R-2 = 0.40, P < 0.001), although soil water content also played a role. Precipitation received during the growing season had a large legacy effect on Fc. Annual weather variation overshadowed the influence of land-use types on Fc. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Pan, Zhanlei; Wei, Zhijun] Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Huhot 010018, Peoples R China. [Pan, Zhanlei; Ma, Lei; Rong, Yuping] China Agr Univ, Anim Sci & Technol Coll, Beijing Key Lab Grassland Sci, Beijing 100193, Peoples R China. [Johnson, Douglas A.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Ma, Lei] Chinese Acad Sci, Inst Atmospher Phys, State Key Lab Atmospher Boundary Layer Phys & Atm, Beijing 100029, Peoples R China. RP Rong, YP (reprint author), China Agr Univ, Anim Sci & Technol Coll, Beijing Key Lab Grassland Sci, Beijing 100193, Peoples R China. EM rongyuping@cau.edu.cn FU National Natural Science Foundation of China [31472136]; National Forage Production System Project in China [CARS-35]; National Key Basic Research Program of China [2014CB138805] FX Research was funded by the National Natural Science Foundation of China (31472136), National Forage Production System Project (CARS-35) in China and National Key Basic Research Program of China (2014CB138805). NR 43 TC 0 Z9 0 U1 25 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD NOV PY 2016 VL 144 BP 160 EP 167 DI 10.1016/j.atmosenv.2016.08.085 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DY7RD UT WOS:000385326100016 ER PT J AU Watkins, BA Kim, J Kenny, A Pedersen, TL Pappan, KL Newman, JW AF Watkins, Bruce A. Kim, Jeffrey Kenny, Anne Pedersen, Theresa L. Pappan, Kirk L. Newman, John W. TI Circulating levels of endocannabinoids and oxylipins altered by dietary lipids in older women are likely associated with previously identified gene targets SO BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS LA English DT Article DE Postmenopausal women; Endocannabinoids; Oxylipins; Global metabolites ID POLYUNSATURATED FATTY-ACIDS; CORONARY-HEART-DISEASE; POSTMENOPAUSAL WOMEN; HUMAN-PLATELETS; OMEGA-3-FATTY-ACID INTAKE; DOCOSAHEXAENOIC ACIDS; FISH-OIL; METABOLISM; SUPPLEMENTATION; METABOLOMICS AB Postmenopausal women (PMW) report marginal n - 3 PUFA intakes and are at risk of chronic diseases associated with the skeletal, muscular, neuroendocrine, and cardiovascular systems. How n - 3 PUFA affect the amounts of endocannabinoids (ECs) and oxylipins (OLs) of metabolic and physiologic importance in PMW is not clear. Based on our recent findings that dietary n - 3 PUFA alter gene targets of the EC system and lower pro-inflammatory OL we proceeded to characterize these actions in blood of PMW. Our aim was to determine levels of the ECs, OLs, and global metabolites (GM) in white PMW (75 +/- 7 y), randomized in a double-masked manner, from baseline to 6 mo after receiving a fish oil supplement of n - 3 PUFA (720 mg 20:5n3 + 480 mg 22:6n3/d, n = 20) or placebo (1.8 g oleic acid/d, n = 20). ECs and OLs in serum were determined by UPLC-MS/MS and GM by GC-MS and LC-MS/MS. Plasma 20:5n3 and 22:6n3 levels increased in PMW given fish oil. EC n - 6 acyl-ethanolamides,arachidonate-derived diols were decreased and 20:5n3 and 22:6n3 diols, epoxides, and alcohols were increased in PMW given fish oil. GM analysis revealed that n - 3 PUFA supplementation increased renal steroid hormone and proteolytic metabolite levels in PMW. Herein, we confirm that gene targets of the EC system, previously found as modifiable by n - 3 PUFA result in changes in the levels of ECs and OLs in PMW. This study shows phenotypic responses (in levels) to n - 3 PUFA supplementation in PMW and increases of n - 3 acyl-ethanolamide and n - 3-derived OL of clinical considerations in aging. (C) 2016 Elsevier B.V. All rights reserved. C1 [Watkins, Bruce A.; Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Watkins, Bruce A.; Kenny, Anne] Univ Connecticut, Ctr Hlth, Ctr Aging, Farmington, CT USA. [Kim, Jeffrey] Univ Calif Davis, Genome & Biomed Sci Facil, Davis, CA 95616 USA. [Pedersen, Theresa L.; Newman, John W.] ARS, USDA, WHNRC, Davis, CA USA. [Pappan, Kirk L.] Metabolon Inc, Durham, NC USA. RP Watkins, BA (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM baw@purdue.edu; jefkim@ucdavis.edu; kenny@uchc.edu; Theresa.Pedersen@ARS.USDA.GOV; KPappan@metabolon.com; John.Newman@ARS.USDA.GOV FU University of Connecticut; UCHC, Center on Aging; USDA [5306-51530-019-00D]; West Coast Metabolomics Center [NIH U24 DK097154] FX Watkins organized the research, performed the FAME analyses and did most of the writing; Kenny collected samples and supervised the human study; Kim conducted the analyses of endocannabinoids and oxylipins under the supervision of Pedersen and Newman; Pappan performed the global metabolite analyses and data analysis was performed by Newman, Watkins and Pappan. All authors reviewed and help revise the manuscript. This research was supported by funds to BAW for the metabolomics collaborations at the University of Connecticut and the UCHC, Center on Aging. Additional support was provided by the USDA Intramural Projects 5306-51530-019-00D and the West Coast Metabolomics Center Grant NIH U24 DK097154. The USDA is an equal opportunity provider and employer. NR 57 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1388-1981 EI 0006-3002 J9 BBA-MOL CELL BIOL L JI Biochim. Biophys. Acta Mol. Cell Biol. Lipids PD NOV PY 2016 VL 1861 IS 11 BP 1693 EP 1704 DI 10.1016/j.bbalip.2016.07.007 PG 12 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA DY7RA UT WOS:000385325800011 PM 27452639 ER PT J AU Wilhelm, C Harrison, O Schmitt, V Pelletier, M Spencer, S Urban, J Siegel, R Belkaid, Y AF Wilhelm, C. Harrison, O. Schmitt, V. Pelletier, M. Spencer, S. Urban, J. Siegel, R. Belkaid, Y. TI FATTY ACID METABOLISM SUSTAINS ILC2 MEDIATED BARRIER IMMUNITY DURING INFECTION AND MALNUTRITION SO CYTOKINE LA English DT Meeting Abstract CT 4th Annual Meeting of The International-Cytokine-and-Interferon-Society (ICIS) CY OCT 16-19, 2016 CL San Francisco, CA SP Int Cytokine & Interferon Soc C1 [Wilhelm, C.; Schmitt, V.] Univ Bonn, Bonn, Germany. [Harrison, O.; Spencer, S.; Siegel, R.; Belkaid, Y.] NIH, Bldg 10, Bethesda, MD 20892 USA. [Pelletier, M.] Univ Laval, Quebec City, PQ, Canada. [Urban, J.] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1043-4666 EI 1096-0023 J9 CYTOKINE JI Cytokine PD NOV PY 2016 VL 87 MA 3P214 BP 152 EP 152 PG 1 WC Biochemistry & Molecular Biology; Cell Biology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Immunology GA DY7PH UT WOS:000385321100247 ER PT J AU Echeverria-Londono, S Newbold, T Hudson, LN Contu, S Hill, SLL Lysenko, I Arbelaez-Cortes, E Armbrecht, I Boekhout, T Cabra-Garcia, J Dominguez-Haydar, Y Nates-Parra, G Gutierrez-Lamus, DL Higuera, D Isaacs-Cubides, PJ Lopez-Quintero, CA Martinez, E Miranda-Esquivel, DR Navarro-Iriarte, LE Noriega, JA Otavo, SE Parra-H, A Poveda, K Ramirez-Pinilla, MP Rey-Velasco, JC Rosselli, L Smith-Pardo, AH Urbina-Cardona, JN Purvis, A AF Echeverria-Londono, Susy Newbold, Tim Hudson, Lawrence N. Contu, Sara Hill, Samantha L. L. Lysenko, Igor Arbelaez-Cortes, Enrique Armbrecht, Inge Boekhout, Teun Cabra-Garcia, Jimmy Dominguez-Haydar, Yamileth Nates-Parra, Guiomar Gutierrez-Lamus, Doris L. Higuera, Diego Johanna Isaacs-Cubides, Paola Lopez-Quintero, Carlos A. Martinez, Eliana Rafael Miranda-Esquivel, Daniel Eduardo Navarro-Iriarte, Luis Ari Noriega, Jorge Eduardo Otavo, Samuel Parra-H, Alejandro Poveda, Katja Ramirez-Pinilla, Martha P. Carlos Rey-Velasco, Juan Rosselli, Loreta Smith-Pardo, Allan H. Nicolas Urbina-Cardona, Jose Purvis, Andy TI Modelling and projecting the response of local assemblage composition to land use change across Colombia SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE community composition; GLMM; hindcast; land use impact; Neotropics; PREDICTS project; representative concentration pathways ID TROPICAL RAIN-FORESTS; BIOTIC HOMOGENIZATION; SECONDARY FORESTS; BIODIVERSITY LOSS; TERRESTRIAL BIODIVERSITY; GLOBAL BIODIVERSITY; SPECIES RICHNESS; MASS EXTINCTION; HABITAT LOSS; CONSERVATION AB Aim Understanding the impact of land use change within assemblages is fundamental to mitigation policies at local and regional scale. Here, we aim to quantify how site-level terrestrial assemblages are responding to land use change in Colombia a mega-diverse country and to project future biodiversity under different scenarios of land use change associated with climate change policies. Location Colombia (northern South America). Methods We collated original biodiversity data from 17 publications (285 sites) that examined how human impact affects terrestrial biodiversity in Colombia. From each site we estimated compositional intactness (i.e. compositional similarity to undisturbed sites). We fitted generalized linear mixed-effects models to estimate how these measures of local biodiversity vary across land use habitats. Using space-for-time substitution, we applied our estimates to hindcast biodiversity changes since 1500 and project future changes under climate change policies of the four representative concentration pathways (RCPs). Results Assemblages in urban, cropland and pasture sites were compositionally very different from those in primary vegetation. We infer that average compositional intactness has been reduced by 18% across Colombia to date, with strong regional variation. The best RCP scenario for future biodiversity is GCAM-RCP4.5, a path that favours the expansion of secondary forests under a strong carbon market; while the worst is MESSAGE-RCP8.5, 'the business-as-usual' scenario. Main conclusions Land use change has driven an increasing change in the composition of ecological assemblages in Colombia. By 2095, the implementation of carbon markets policy of climate change from GCAM-RCP4.5 could mitigate these changes in community composition. In contrast, the business-as-usual scenario MESSAGE-RCP8.5 predicts a steep community change placing the quality of ecosystems at risk. C1 [Echeverria-Londono, Susy; Hudson, Lawrence N.; Contu, Sara; Hill, Samantha L. L.; Purvis, Andy] Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. [Echeverria-Londono, Susy; Lysenko, Igor; Purvis, Andy] Imperial Coll London, Dept Life Sci, Buckhurst Rd, Ascot SL5 7QN, Berks, England. [Newbold, Tim; Hill, Samantha L. L.] United Nations Environm Programme World Conservat, 219 Huntingdon Rd, Cambridge CB3 0DL, England. [Newbold, Tim] UCL, Dept Genet Evolut & Environm, Ctr Biodivers & Environm Res, Gower St, London WC1E 6BT, England. [Arbelaez-Cortes, Enrique] Inst Invest Recursos Biol Alexander von Humboldt, Colecc Tejidos, Km 17 Carretera Cali Palmira CIAT, Valle Del Cauca, Colombia. [Arbelaez-Cortes, Enrique] Univ Ind Santander, Grp Estudios Biodiversidad, Escuela Biol, Bucaramanga, Colombia. [Armbrecht, Inge] Univ Valle, Dept Biol, Apartado Aereo 25360, Cali, Colombia. [Boekhout, Teun] CBS Fungal Biodivers Ctr CBS KNAW, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands. [Cabra-Garcia, Jimmy] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo, SP, Brazil. [Cabra-Garcia, Jimmy] Univ Valle, Dept Biol, Secc Entomol, Grp Invest Biol Ecol & Manejo Hormigas, Cali 25360, Colombia. [Dominguez-Haydar, Yamileth] Univ Atlantico, Programa Biol, Km 7 Via Puerto Colombia, Atlantico, Colombia. [Nates-Parra, Guiomar] Univ Nacl Cordoba, Dept Biol, Carrera 6 76-103, Monteria, Colombia. [Gutierrez-Lamus, Doris L.] Inst Amazonico Invest Cient Sinchi, Grp Fauna Amazon, Leticia, Colombia. [Higuera, Diego] Corp Sentido Nat, Carrera 70H 122 A-98,Apartamento 101, Bogota, DC, Colombia. [Johanna Isaacs-Cubides, Paola] Pontificia Univ Javeriana, Lab Herpetol, Bogota, DC, Colombia. [Lopez-Quintero, Carlos A.] Univ Antioquia, Inst Biol, Lab TEHO, POB 1226,Calle 67 N 53-108, Medellin, Colombia. [Martinez, Eliana] Univ Nacl Colombia, Dept Biol, Bogota 7945, DC, Colombia. [Rafael Miranda-Esquivel, Daniel; Carlos Rey-Velasco, Juan] Univ Ind Santander, Escuela Biol, Colecc Entomol, Lab Sistemat & Biogeog, Bucaramanga, Colombia. [Eduardo Navarro-Iriarte, Luis] Univ Magdalena, San Andres De Sotavento, Colombia. [Ari Noriega, Jorge] Univ Los Andes, Lab Zool & Ecol Acuat LAZOEA, Bogota, DC, Colombia. [Eduardo Otavo, Samuel] Univ Concepcion, Fac Ciencias Forestales, Lab Ecol Paisaje, Concepcion, Chile. [Parra-H, Alejandro] Corp Gest Serv Ecosistem Polinizac & Abejas SEPyA, Bogota, DC, Colombia. [Parra-H, Alejandro] Univ Nacl Colombia, Dept Biol, Lab Invest Abejas LABUN, Carrera 45 26-85, Bogota, DC, Colombia. [Poveda, Katja] Cornell Univ, Dept Entomol, 4126 Comstock Hall, Ithaca, NY 14853 USA. [Ramirez-Pinilla, Martha P.] Univ Ind Santander, Colecc Herpetol, Bucaramanga, Colombia. [Ramirez-Pinilla, Martha P.] Univ Ind Santander, Grp Estudios Biodiversidad, Escuela Biol, Lab Biol Reprod Vertebrados, Bucaramanga, Colombia. [Carlos Rey-Velasco, Juan] Inst Invest Recursos Biol Alexander von Humboldt, Calle 28 N 15-09, Bogota, DC, Colombia. [Rosselli, Loreta] Univ Ciencias Aplicadas & Ambientales, Fac Ciencias Ambientales, Bogota, DC, Colombia. [Smith-Pardo, Allan H.] USDA, San Francisco, CA USA. [Smith-Pardo, Allan H.] Univ Nacl Colombia, Sede Medellin UNALMED, Fac Ciencias, Medellin, Colombia. [Nicolas Urbina-Cardona, Jose] Pontificia Univ Javeriana, Dept Ecol & Terr, Bogota, DC, Colombia. RP Echeverria-Londono, S (reprint author), Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. EM susye@nhm.ac.uk RI Newbold, Tim/D-1524-2012; Purvis, Andy/A-7529-2008; OI Newbold, Tim/0000-0001-7361-0051; Purvis, Andy/0000-0002-8609-6204; Echeverria-londono, Susy/0000-0002-0038-146X FU UK Natural Environment Research Council (NERC) [NE/J011193/2]; PREDICTS project; Colciencias (Administrative department of Science, Technology and Innovation of Colombia) FX We thank three anonymous referees and the associate editor Piero Visconti for greatly improving the content and framing of this article. We thank Shai Meiri for sharing the reptile species richness information, and the IMAGE, HYDE, MESSAGE and GCAM teams, especially Allison Thomson, for sharing additional data from their integrated assessment models. This study was supported by the UK Natural Environment Research Council (NERC, grant number: NE/J011193/2), who fund the PREDICTS project (http://www.predicts.org.uk/), and Colciencias (Administrative department of Science, Technology and Innovation of Colombia) who fund S.E.-L.'s PhD programme. PREDICTS is endorsed by the GEO BON. This is a contribution from the Imperial College Grand Challenges in Ecosystems and the Environment Initiative. NR 77 TC 1 Z9 1 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD NOV PY 2016 VL 22 IS 11 BP 1099 EP 1111 DI 10.1111/ddi.12478 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DZ0TJ UT WOS:000385552300003 ER PT J AU Dile, YT Daggupati, P George, C Srinivasan, R Arnold, J AF Dile, Yihun T. Daggupati, Prasad George, Chris Srinivasan, Raghavan Arnold, Jeff TI Introducing a new open source GIS user interface for the SWAT model SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Open source software; QGIS; QSWAT; SWAT; Gumera watershed; Ethiopia ID DOWNSTREAM ECOSYSTEM SERVICES; MESSAGE-PASSING INTERFACE; WATER-QUALITY; UNCERTAINTY; FLOW; OPPORTUNITIES; CALIBRATION AB The Soil and Water Assessment Tool (SWAT) model is a robust watershed modeling tool. It typically uses the ArcSWAT interface to create its inputs. ArcSWAT is public domain software which works in the licensed ArcGIS environment. The aim of this paper was to develop an open source user interface for the SWAT model. The interface, QSWAT, is written in the Python programming language and uses various functionalities of the open source geographic information system, QGIS. The current interface performs similar functions to ArcSWAT, but with additional enhanced features such as merging small subbasins and static and dynamic visualization of outputs. The interface is demonstrated through a case study in the Gumera watershed in the Lake Tana basin of Ethiopia, where it showed a successful performance. QSWAT will be a valuable tool for the SWAT scientific community, with improved availability and functionality compared with other options for creating SWAT models. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Dile, Yihun T.; Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, Ecosyst Sci & Management Dept, College Stn, TX 77801 USA. [Daggupati, Prasad] Univ Guelph, Sch Engn, Guelph, ON, Canada. [George, Chris] United Nations Univ, Int Inst Software Technol, Macau, Peoples R China. [Arnold, Jeff] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. RP Dile, YT (reprint author), Texas A&M Univ, Spatial Sci Lab, Ecosyst Sci & Management Dept, College Stn, TX 77801 USA. EM yihundile@tamu.edu RI Daggupati, Prasad/D-8886-2017; OI Daggupati, Prasad/0000-0002-7044-3435; Srinivasan, Raghavan/0000-0001-8375-6038; Dile, Yihun/0000-0002-3419-2018 FU Feed the Future through the U.S. Agency for International Development [AID-OAA-A-13-0005] FX This publication was partly made possible through support provided by Feed the Future through the U.S. Agency for International Development, under the terms of Contract No.AID-OAA-A-13-0005. The opinions expressed herein are those of the authors and do not necessarily reflect the views of the U.S. Agency for International Development. We thank the Ethiopian National Meteorological Services Agency and the Ethiopian Ministry of Water, Energy, and Irrigation for providing us with observed weather and streamflow data, respectively. Comments from three anonymous reviewers truly helped to improve the manuscript. We appreciate them. NR 44 TC 0 Z9 0 U1 37 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD NOV PY 2016 VL 85 BP 129 EP 138 DI 10.1016/j.envsoft.2016.08.004 PG 10 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DZ1JX UT WOS:000385595800010 ER PT J AU Confalonieri, R Bregaglio, S Adam, M Ruget, F Li, T Hasegawa, T Yin, XY Zhu, Y Boote, K Buis, S Fumoto, T Gaydon, D Lafarge, T Marcaida, M Nakagawa, H Ruane, AC Singh, B Singh, U Tang, L Tao, FL Fugice, J Yoshida, H Zhang, Z Wilson, LT Baker, J Yang, YB Masutomi, Y Wallach, D Acutis, M Bouman, B AF Confalonieri, Roberto Bregaglio, Simone Adam, Myriam Ruget, Francoise Li, Tao Hasegawa, Toshihiro Yin, Xinyou Zhu, Yan Boote, Kenneth Buis, Samuel Fumoto, Tamon Gaydon, Donald Lafarge, Tanguy Marcaida, Manuel Nakagawa, Hiroshi Ruane, Alex C. Singh, Balwinder Singh, Upendra Tang, Liang Tao, Fulu Fugice, Job Yoshida, Hiroe Zhang, Zhao Wilson, Lloyd T. Baker, Jeff Yang, Yubin Masutomi, Yuji Wallach, Daniel Acutis, Marco Bouman, Bas TI A taxonomy-based approach to shed light on the babel of mathematical models for rice simulation SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Model classification; Model parameterisation; Model ensemble; Model structure; Rice; Uncertainty ID CROP MODELS; SENSITIVITY-ANALYSIS; CLIMATE-CHANGE; CALIBRATION; YIELD; WHEAT; UNCERTAINTY; WATER; PLASTICITY; EVOLUTION AB For most biophysical domains, differences in model structures are seldom quantified. Here, we used a taxonomy-based approach to characterise thirteen rice models. Classification keys and binary attributes for each key were identified, and models were categorised into five clusters using a binary similarity measure and the unweighted pair-group method with arithmetic mean. Principal component analysis was performed on model outputs at four sites. Results indicated that (i) differences in structure often resulted in similar predictions and (ii) similar structures can lead to large differences in model outputs. User subjectivity during calibration may have hidden expected relationships between model structure and behaviour. This explanation, if confirmed, highlights the need for shared protocols to reduce the degrees of freedom during calibration, and to limit, in turn, the risk that user subjectivity influences model performance. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Confalonieri, Roberto; Bregaglio, Simone; Acutis, Marco] Univ Milan, Cassandra Lab, I-20122 Milan, Italy. [Adam, Myriam; Lafarge, Tanguy] CIRAD, UMR AGAP, F-34398 Montpellier, France. [Ruget, Francoise; Buis, Samuel] INRA, EMMAH UMR1114, F-84914 Avignon, France. [Li, Tao; Marcaida, Manuel; Bouman, Bas] Int Rice Res Inst, Los Banos, Philippines. [Hasegawa, Toshihiro; Fumoto, Tamon] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki, Japan. [Yin, Xinyou] Wageningen Univ, Ctr Crop Syst Anal, NL-6700 AP Wageningen, Netherlands. [Zhu, Yan; Tang, Liang] Nanjing Agr Univ, Natl Engn & Technol Ctr Informat Agr, Jiangsu Key Lab Informat Agr, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Nanjing, Jiangsu, Peoples R China. [Boote, Kenneth] Univ Florida, Gainesville, FL USA. [Gaydon, Donald] CSIRO Agr & Food, Brisbane, Qld, Australia. [Nakagawa, Hiroshi; Yoshida, Hiroe] Natl Agr & Food Res Org, Tsukuba, Ibaraki, Japan. [Ruane, Alex C.] NASA, Goddard Inst Space Studies, New York, NY USA. [Singh, Upendra; Fugice, Job] Int Fertilizer Dev Ctr, Muscle Shoals, AL 35662 USA. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100864, Peoples R China. [Zhang, Zhao] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Wilson, Lloyd T.; Yang, Yubin] Texas A&M AgriLife Res & Extens Ctr, Beaumont, TX USA. [Baker, Jeff] ARS, USDA, Big Spring, TX USA. [Masutomi, Yuji] Ibaraki Univ, Coll Agr, Mito, Ibaraki, Japan. [Wallach, Daniel] INRA, UMR1248, Agrosyst & Dev Terr, F-31326 Castanet Tolosan, France. [Singh, Balwinder] CIMMYT, CG Block,NASC Complex, New Delhi 110012, India. [Tao, Fulu] Nat Resources Inst Finland Luke, Vantaa 01301, Finland. [Li, Tao] AgMIP Rice Team, Tsukuba, Ibaraki, Japan. RP Confalonieri, R (reprint author), Univ Milan, Cassandra Lab, I-20122 Milan, Italy. EM roberto.confalonieri@unimi.it RI Gaydon, Donald /F-4608-2012; OI Wallach, Daniel/0000-0003-3500-8179; Hasegawa, Toshihiro/0000-0001-8501-5612; , Balwinder-Singh/0000-0002-6715-2207 FU National Science Foundation of China [31561143003]; Academy of Finland, PLUMES project [277403]; MAFF; Global Environment Research of MOE, Japan [S-10-2]; National High Tech Research and Development Program of China [2013AA100404]; EC-FP7 [613817] FX FT is supported by the National Science Foundation of China (31561143003) and the Academy of Finland, PLUMES project (277403). TH's participation was supported by MAFF and the Global Environment Research (S-10-2) of MOE, Japan. YZ's work was supported by the National High Tech Research and Development Program of China (2013AA100404). RC was partly supported by EC-FP7 under Grant Agreement No. 613817 (MODEXTREME). Nanjing Agricultural University, International Rice Research Institute, Ministry of Agriculture, Forestry and Fisheries and Ministry of the Environment of Japan, and Cassandra lab. of the University of Milan supported the annual research planning meetings of the AgMIP Rice Team. USDA is an equal opportunity provider and employer. NR 56 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD NOV PY 2016 VL 85 BP 332 EP 341 DI 10.1016/j.envsoft.2016.09.007 PG 10 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA DZ1JX UT WOS:000385595800024 ER PT J AU Bello, NM Kramer, M Tempelman, RJ Stroup, WW St-Pierre, NR Craig, BA Young, LJ Gbur, EE AF Bello, Nora M. Kramer, Matthew Tempelman, Robert J. Stroup, Walter W. St-Pierre, Normand R. Craig, Bruce A. Young, Linda J. Gbur, Edward E. TI Short communication: On recognizing the proper experimental unit in animal studies in the dairy sciences SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE experimental unit; replication; observational unit; hierarchical data structure; pen ID POLICY AB Sound design of experiments combined with proper implementation of appropriate statistical methods for data analysis are critical for producing meaningful scientific results that are both replicable and reproducible. This communication addresses specific aspects of design and analysis of experiments relevant to the dairy sciences and, in so doing, responds to recent concerns raised in a letter to the editor of the Journal of Dairy Science regarding journal policy for research publications on pen-based animal studies. We further elaborate on points raised, rectify interpretation of important concepts, and show how aspects of statistical inference and elicitation of research conclusions are affected. C1 [Bello, Nora M.] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [Kramer, Matthew] ARS, USDA, Beltsville, MD 20705 USA. [Tempelman, Robert J.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Stroup, Walter W.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [St-Pierre, Normand R.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Craig, Bruce A.] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Young, Linda J.] Natl Agr Stat Serv, Washington, DC 20250 USA. [Gbur, Edward E.] Univ Arkansas, Agr Stat Lab, Fayetteville, AR 72701 USA. RP Bello, NM (reprint author), Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. EM nbello@ksu.edu RI Craig, Bruce/D-5797-2017 OI Craig, Bruce/0000-0001-9346-467X NR 16 TC 1 Z9 1 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD NOV PY 2016 VL 99 IS 11 BP 8871 EP 8879 DI 10.3168/jds.2016-11516 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DZ1PQ UT WOS:000385610700034 PM 27614832 ER PT J AU Krueger, LA Beitz, DC Humphrey, SB Stabel, JR AF Krueger, L. A. Beitz, D. C. Humphrey, S. B. Stabel, J. R. TI Gamma delta T cells are early responders to Mycobacterium avium ssp paratuberculosis in colostrum-replete Holstein calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy calf; colostrum; vitamins; cellular immunity ID SUBSP PARATUBERCULOSIS; IMMUNE-RESPONSE; DAIRY CALVES; MILK; INFECTION; GROWTH; HAPTOGLOBIN; LACTATION; INDUCTION; REPLACER AB Peripheral blood mononuclear cells (PBMC) and mesenteric node lymphocytes (MNL) were obtained from 30 calves that were assigned randomly at birth to 1 of 6 treatment groups with 5 calves per treatment in a 14-d study: (1) colostrum-deprived (CD), no vitamins; (2) colostrum-replacer (CR), no vitamins; (3) CR, vitamin A; (4) CR, vitamin D-3; (5) CR, vitamin E; (6) CR, vitamins A, D-3, E. Calves were injected with appropriate vitamin supplements and fed pasteurized whole milk (CD calves) or fractionated colostrum-replacer (CR calves) at birth. Thereafter, all calves were fed pasteurized whole milk fortified with vitamins according to treatment group. Calves were orally inoculated with 10(8) cfu of Mycobacterium avium ssp. paratuberculosis (MAP) on d 1 and 3. The PBMC and MNL harvested on d 13 were analyzed by flow cytometry as fresh cells, after 3-d culture with phytohemagglutinin (PHA), and after 6-d culture with a whole-cell sonicate of MAP (MPS). Peripheral gamma delta T cells were a predominant lymphocyte subset in neonatal calves, with a decreased percentage noted in CD calves compared with CR calves. As well, CD25 expression was higher in gamma delta T cells compared with other cell subsets, regardless of treatment group. Stimulation of PBMC with PHA resulted in increased CD4(+) and CD8(+) subsets, whereas MNL response was dominated by expansion of B-cell subpopulations. Stimulation with PHA and MPS decreased the relative abundance of PBMC gamma delta T cells, but MNL gamma delta T cells increased upon stimulation with MPS. These results identify gamma delta T cells as key early responders to intracellular infection in neonatal calves and suggest that colostrum may be an important mediator of this response. C1 [Krueger, L. A.; Beitz, D. C.; Stabel, J. R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Krueger, L. A.; Stabel, J. R.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Humphrey, S. B.] ARS, Microscopy Serv, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Stabel, JR (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA.; Stabel, JR (reprint author), ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM judy.stabel@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD NOV PY 2016 VL 99 IS 11 BP 9040 EP 9050 DI 10.3168/jds.2016-11144 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DZ1PQ UT WOS:000385610700049 PM 27614838 ER PT J AU Niederwerder, MC Nietfeld, JC Bai, JF Peddireddi, L Breazeale, B Anderson, J Kerrigan, MA An, BY Oberst, RD Crawford, K Lager, KM Madson, DM Rowland, RRR Anderson, GA Hesse, RA AF Niederwerder, Megan C. Nietfeld, Jerome C. Bai, Jianfa Peddireddi, Lalitha Breazeale, Barbara Anderson, Joe Kerrigan, Maureen A. An, Baoyan Oberst, Richard D. Crawford, Kimberly Lager, Kelly M. Madson, Darin M. Rowland, Raymond R. R. Anderson, Gary A. Hesse, Richard A. TI Tissue localization, shedding, virus carriage, antibody response, and aerosol transmission of Porcine epidemic diarrhea virus following inoculation of 4-week-old feeder pigs SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Coronavirus; diarrhea; Porcine epidemic diarrhea virus; swine; transmission ID UNITED-STATES; INFECTION AB We determined tissue localization, shedding patterns, virus carriage, antibody response, and aerosol transmission of Porcine epidemic diarrhea virus (PEDV) following inoculation of 4-week-old feeder pigs. Thirty-three pigs were randomly assigned to 1 of 3 groups for the 42-day study: inoculated (group A; n = 23), contact transmission (group B; n = 5), and aerosol transmission (group C; n = 5). Contact transmission occurred rapidly to group B pigs whereas productive aerosol transmission failed to occur to group C pigs. Emesis was the first clinical sign noted at 3 days postinoculation (dpi) followed by mild to moderate diarrhea lasting 5 more days. Real-time PCR detected PEDV in fecal and nasal swabs, oral fluids, serum, and gastrointestinal and lymphoid tissues. Shedding occurred primarily during the first 2 weeks postinoculation, peaking at 5-6 dpi; however, some pigs had PEDV nucleic acid detected in swabs collected at 21 and 28 dpi. Antibody titers were measurable between 14 and 42 dpi. Although feces and intestines collected at 42 dpi were PEDV negative by PCR and immunohistochemistry, respectively, small intestines from 70% of group A pigs were PCR positive. Although disease was relatively mild and transient in this age group, the results demonstrate that 4-week-old pigs are productively infected and can sustain virus replication for several weeks. Long-term shedding of PEDV in subclinically affected pigs should be considered an important source for PEDV transmission. C1 [Niederwerder, Megan C.; Nietfeld, Jerome C.; Bai, Jianfa; Peddireddi, Lalitha; Kerrigan, Maureen A.; Oberst, Richard D.; Rowland, Raymond R. R.; Anderson, Gary A.; Hesse, Richard A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, 1800 Denison Ave, Manhattan, KS 66506 USA. [Niederwerder, Megan C.; Nietfeld, Jerome C.; Bai, Jianfa; Peddireddi, Lalitha; Breazeale, Barbara; Anderson, Joe; An, Baoyan; Oberst, Richard D.; Anderson, Gary A.; Hesse, Richard A.] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, 1800 Denison Ave, Manhattan, KS 66506 USA. [Crawford, Kimberly; Lager, Kelly M.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [Madson, Darin M.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. RP Niederwerder, MC (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, 1800 Denison Ave, Manhattan, KS 66506 USA.; Niederwerder, MC (reprint author), Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, 1800 Denison Ave, Manhattan, KS 66506 USA. EM mniederwerder@vet.k-state.edu FU National Pork Board [13-228] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by the National Pork Board award number 13-228. NR 13 TC 0 Z9 0 U1 6 U2 6 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD NOV PY 2016 VL 28 IS 6 BP 671 EP 678 DI 10.1177/1040638716663251 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DZ4CM UT WOS:000385805400009 PM 27698163 ER PT J AU Pantin-Jackwood, MJ Costa-Hurtado, M Shepherd, E DeJesus, E Smith, D Spackman, E Kapczynski, DR Suarez, DL Stallknecht, DE Swayne, DE AF Pantin-Jackwood, Mary J. Costa-Hurtado, Mar Shepherd, Eric DeJesus, Eric Smith, Diane Spackman, Erica Kapczynski, Darrell R. Suarez, David L. Stallknecht, David E. Swayne, David E. TI Pathogenicity and Transmission of H5 and H7 Highly Pathogenic Avian Influenza Viruses in Mallards SO JOURNAL OF VIROLOGY LA English DT Article ID DUCKS ANAS-PLATYRHYNCHOS; WILD BIRDS; EXPERIMENTAL-INFECTION; MIGRATORY BIRDS; DOMESTIC DUCKS; A VIRUSES; NATURAL INFECTION; POULTRY OUTBREAKS; CAIRINA-MOSCHATA; MUSCOVY DUCKS AB Wild aquatic birds have been associated with the intercontinental spread of H5 subtype highly pathogenic avian influenza (HPAI) viruses of the A/goose/Guangdong/1/96 (Gs/GD) lineage during 2005, 2010, and 2014, but dispersion by wild waterfowl has not been implicated with spread of other HPAI viruses. To better understand why Gs/GD H5 HPAI viruses infect and transmit more efficiently in waterfowl than other HPAI viruses, groups of mallard ducks were challenged with one of 14 different H5 and H7 HPAI viruses, including a Gs/GD lineage H5N1 (clade 2.2) virus from Mongolia, part of the 2005 dispersion, and the H5N8 and H5N2 index HPAI viruses (clade 2.3.4.4) from the United States, part of the 2014 dispersion. All virus-inoculated ducks and contact exposed ducks became infected and shed moderate to high titers of the viruses, with the exception that mallards were resistant to Ck/Pennsylvania/83 and Ck/Queretaro/95 H5N2 HPAI virus infection. Clinical signs were only observed in ducks challenged with the H5N1 2005 virus, which all died, and with the H5N8 and H5N2 2014 viruses, which had decreased weight gain and fever. These three viruses were also shed in higher titers by the ducks, which could facilitate virus transmission and spread. This study highlights the possible role of wild waterfowl in the spread of HPAI viruses. C1 [Pantin-Jackwood, Mary J.; Costa-Hurtado, Mar; Shepherd, Eric; DeJesus, Eric; Smith, Diane; Spackman, Erica; Kapczynski, Darrell R.; Suarez, David L.; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. [Stallknecht, David E.] Univ Georgia, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Res Grp Infect Dis Prod Anim, St Hyacinthe, PQ, Canada. [Costa-Hurtado, Mar] Univ Montreal, Fac Vet Med, Swine & Poultry Infect Dis Res Ctr, St Hyacinthe, PQ, Canada. [Spackman, Erica] Univ Georgia, Coll Vet Med, Poultry Diagnost & Res Ctr, Athens, GA USA. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU USDA/ARS CRIS project [6612-32000-063-00D]; Center for Research on Influenza Pathogenesis (CRIP); NIAID [HHSN272201400008C] FX This research was supported by USDA/ARS CRIS project 6612-32000-063-00D and by the Center for Research on Influenza Pathogenesis (CRIP) and the NIAID-funded Center of Excellence in Influenza Research and Surveillance (CEIRS; contract HHSN272201400008C). NR 77 TC 3 Z9 3 U1 13 U2 13 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD NOV PY 2016 VL 90 IS 21 BP 9967 EP 9982 DI 10.1128/JVI.01165-16 PG 16 WC Virology SC Virology GA DZ0JW UT WOS:000385525700035 PM 27558429 ER PT J AU Asztalos, IB Gleason, JA Sever, S Gedik, R Asztalos, BF Horvath, KV Dansinger, ML Lamon-Fava, S Schaefer, EJ AF Asztalos, Ivor B. Gleason, Joi A. Sever, Sakine Gedik, Reyhan Asztalos, Bela F. Horvath, Katalin V. Dansinger, Michael L. Lamon-Fava, Stefania Schaefer, Ernst J. TI Effects of eicosapentaenoic acid and docosahexaenoic acid on cardiovascular disease risk factors: a randomized clinical trial SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article DE Omega-3 fatty acids; Fish oils; Triglycerides; Lp-PLA(2) ID CORONARY-ARTERY-DISEASE; N-3 FATTY-ACIDS; PHOSPHOLIPASE A(2); HEART-DISEASE; HYPERCHOLESTEROLEMIC PATIENTS; PRIMARY PREVENTION; ICOSAPENT ETHYL; RICH OIL; LIPOPROTEIN; PLASMA AB Background. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), the primary omega-3 fatty acids in fish oil, have been shown to reduce cardiovascular disease (CVD) risk. Objective. This study aimed to examine the independent effects of EPA and DHA on lipid and apolipoprotein levels, as well as on inflammatory biomarkers of CVD risk, using doses often used in the general population. Design. A blinded, randomized 6-week trial was performed in 121 healthy, normolipidemic subjects who received olive oil placebo 6 g/d, EPA 600 mg/d, EPA 1800 mg/d, or DHA 600 mg/d. The EPA was derived from genetically modified yeast. Results. The subjects tolerated the supplements well with no safety issues; and the expected treatment-specific increases in plasma EPA and DHA levels were observed. Compared to placebo, the DHA group had significant decreases in postprandial triglyceride (TG) concentrations (-20%, -52.2 mg/dL, P = 0.03), significant increases in fasting and postprandial low-density lipoprotein cholesterol (LDL-C) (+18.4%, 17.1 mg/dL, P = 0.001), with no significant changes in inflammatory biomarkers. No significant effects were observed in the EPA 600 mg/d group. The high-dose EPA group had significant decreases in lipoprotein-associated phospholipase A2 concentrations (Lp-PLA(2)) (-14.1%, -21.4 ng/mL, P = 0.003). Conclusions. The beneficial effects of EPA 1800 mg/d on CVD risk reduction may relate in part to the lowering of Lp-PLA(2) withbut adversely affecting LDL-C. In contrast, DHA decreased postprandial TG, but raised LDL-C. Our observations indicate that these dietary fatty acids have divergent effects on cardiovascular risk markers. (C) 2016 Published by Elsevier Inc. C1 [Asztalos, Ivor B.; Gleason, Joi A.; Sever, Sakine; Gedik, Reyhan; Dansinger, Michael L.; Schaefer, Ernst J.] Cardiovasc Res Associates, Boston, MA USA. [Asztalos, Ivor B.; Gleason, Joi A.; Asztalos, Bela F.; Horvath, Katalin V.; Dansinger, Michael L.; Lamon-Fava, Stefania; Schaefer, Ernst J.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. [Dansinger, Michael L.] Tufts Med Ctr, Div Endocrinol & Metab, Boston, MA USA. [Asztalos, Ivor B.] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. RP Schaefer, EJ (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM emst.schaefer@tufts.edu FU NHLBI NIH HHS [P50 HL083813] NR 48 TC 0 Z9 0 U1 9 U2 9 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 EI 1532-8600 J9 METABOLISM JI Metab.-Clin. Exp. PD NOV PY 2016 VL 65 IS 11 BP 1636 EP 1645 DI 10.1016/j.metabol.2016.07.010 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DZ6TB UT WOS:000385993700007 PM 27733252 ER PT J AU Shine, MB Yang, JW El-Habbak, M Nagyabhyru, P Fu, DQ Navarre, D Ghabrial, S Kachroo, P Kachroo, A AF Shine, M. B. Yang, Jung-Wook El-Habbak, Mohamed Nagyabhyru, Padmaja Fu, Da-Qi Navarre, Duroy Ghabrial, Said Kachroo, Pradeep Kachroo, Aardra TI Cooperative functioning between phenylalanine ammonia lyase and isochorismate synthase activities contributes to salicylic acid biosynthesis in soybean SO NEW PHYTOLOGIST LA English DT Article DE isochorismate synthase (ICS); pathogen defense; phenylalanine ammonia lyase (PAL); salicylic acid (SA); soybean (Glycine max) ID SYSTEMIC ACQUIRED-RESISTANCE; RACE-SPECIFIC RESISTANCE; SYRINGAE PV GLYCINEA; PSEUDOMONAS-SYRINGAE; PHYTOPHTHORA RESISTANCE; PERONOSPORA-PARASITICA; AVIRULENCE GENE; PLANT DEFENSE; BENZOIC-ACID; AMINO-ACID AB Salicylic acid (SA), an essential regulator of plant defense, is derived from chorismate via either the phenylalanine ammonia lyase (PAL) or the isochorismate synthase (ICS) catalyzed steps. The ICS pathway is thought to be the primary contributor of defense-related SA, at least in Arabidopsis. We investigated the relative contributions of PAL and ICS to defense-related SA accumulation in soybean (Glycine max). Soybean plants silenced for five PAL isoforms or two ICS isoforms were analyzed for SA concentrations and SA-derived defense responses to the hemibiotrophic pathogens Pseudomonas syringae and Phytophthora sojae. We show that, unlike in Arabidopsis, PAL and ICS pathways are equally important for pathogen-induced SA biosynthesis in soybean. Knock-down of either pathway shuts down SA biosynthesis and abrogates pathogen resistance. Moreover, unlike in Arabidopsis, pathogen infection is associated with the suppression of ICS gene expression. Pathogen-induced biosynthesis of SA via the PAL pathway correlates inversely with phenylalanine concentrations. Although infections with either virulent or avirulent strains of the pathogens increase SA concentrations, resistance protein-mediated response to avirulent P.sojae strains may function in an SA-independent manner. These results show that PAL- and ICS-catalyzed reactions function cooperatively in soybean defense and highlight the importance of PAL in pathogen-induced SA biosynthesis. C1 [Shine, M. B.; Yang, Jung-Wook; El-Habbak, Mohamed; Nagyabhyru, Padmaja; Fu, Da-Qi; Ghabrial, Said; Kachroo, Pradeep; Kachroo, Aardra] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Navarre, Duroy] Washington State Univ, Dept Plant Pathol, USDA, ARS, Prosser, WA 99350 USA. RP Kachroo, A (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM apkach2@uky.edu FU Kentucky Soybean Promotion Board; Kentucky Agricultural Experiment Station [16-12-049] FX We thank Adam Bogdanove and Massimo Delledone (providing Psg strains), Scott Abney (providing P.sojae isolates), Todd Pfeiffer (for help with generating the soybean seed stock) and Amy Crume (for management of the plant growth facilities) for their generous help. This work was supported by grants from the Kentucky Soybean Promotion Board. The information reported in this article (no. 16-12-049) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. NR 55 TC 0 Z9 0 U1 21 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD NOV PY 2016 VL 212 IS 3 BP 627 EP 636 DI 10.1111/nph.14078 PG 10 WC Plant Sciences SC Plant Sciences GA DZ4AE UT WOS:000385797800012 PM 27411159 ER PT J AU Matsubayashi, M Minoura, C Kimura, S Tani, H Furuya, M Lillehoj, HS Matsuda, H Takenaka, S Hatta, T Tsuji, N Sasai, K AF Matsubayashi, Makoto Minoura, Chisa Kimura, Shintaro Tani, Hiroyuki Furuya, Masaru Lillehoj, Hyun S. Matsuda, Haruo Takenaka, Shigeo Hatta, Takeshi Tsuji, Naotoshi Sasai, Kazumi TI Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody SO PARASITOLOGY RESEARCH LA English DT Article DE Chicken monoclonal antibody; Conoid; Eimeria acervulina; Elongation factor-1 alpha ID HOST-CELL INVASION; CRYPTOSPORIDIUM-PARVUM; APICAL ORGANELLES; IN-VITRO; SPOROZOITES; PROTEIN; TRANSHYDROGENASE; CYTOSKELETON; APICOMPLEXA; TOXOPLASMA AB In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1 alpha (EF-1 alpha). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1 alpha and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1 alpha could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites. C1 [Matsubayashi, Makoto; Minoura, Chisa; Kimura, Shintaro; Tani, Hiroyuki; Furuya, Masaru; Takenaka, Shigeo; Sasai, Kazumi] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Div Vet Sci, 1-58 Rinku Orai Kita, Izumisano, Osaka 5988531, Japan. [Lillehoj, Hyun S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Matsuda, Haruo] Hiroshima Univ, Grad Sch Biosphere Sci, Dept Mol & Appl Biosci, Immunobiol Lab, 1-4-4 Kagamiyama, Hiroshima 7398528, Japan. [Hatta, Takeshi; Tsuji, Naotoshi] Kitasato Univ, Dept Parasitol, Sch Med, Minami Ku, 1-15-1 Kitasato, Sagamihara, Kanagawa 2520374, Japan. RP Sasai, K (reprint author), Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Div Vet Sci, 1-58 Rinku Orai Kita, Izumisano, Osaka 5988531, Japan. EM ksasai@vet.osakafu-u.ac.jp FU Ministry of Education, Culture, Sports, and Science [23580445, 25450436] FX We are grateful to Dr. Y. Fukuta, from the APRO Life Science Institute, Inc., for the LC-MS/MS analyses. This study was supported in part by the Grants-in-aid for Scientific Research 23580445 (to K. S., H. T., M. F., and M. M.) and 25450436 (to M. M.) from the Ministry of Education, Culture, Sports, and Science. NR 25 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD NOV PY 2016 VL 115 IS 11 BP 4123 EP 4128 DI 10.1007/s00436-016-5185-0 PG 6 WC Parasitology SC Parasitology GA DY5LU UT WOS:000385142100006 PM 27384177 ER PT J AU Vemmer, M Schumann, M Beitzen-Heineke, W French, BW Vidal, S Patel, AV AF Vemmer, Marina Schumann, Mario Beitzen-Heineke, Wilhelm French, Bryan W. Vidal, Stefan Patel, Anant V. TI Development of a CO2-releasing coformulation based on starch, Saccharomyces cerevisiae and Beauveria bassiana attractive towards western corn rootworm larvae SO PEST MANAGEMENT SCIENCE LA English DT Article DE carbon dioxide; encapsulation; alginate; attract-and-kill; Diabrotica virgifera virgifera ID WIREWORMS COLEOPTERA-ELATERIDAE; CARBON-DIOXIDE; HOST LOCATION; FEEDING STIMULANTS; CHRYSOMELIDAE LARVAE; BEHAVIORAL-RESPONSES; INOCULANT CARRIERS; ETHANOL-PRODUCTION; KILL STRATEGY; PLANT-GROWTH AB BACKGROUNDCO(2) is known as an attractant for many soil-dwelling pests. To implement an attract-and-kill strategy for soil pest control, CO2-emitting formulations need to be developed. The aim of the present work was to develop a slow-release bead system in order to bridge the gap between application and hatching of western corn rootworm larvae. RESULTSWe compared different Ca-alginate beads containing Saccharomyces cerevisiae for their potential to release CO2 over a period of several weeks. The addition of starch improved CO2 release, resulting in significantly higher CO2 concentrations in soil for at least 4 weeks. The missing amylase activity was compensated for either by microorganisms present in the soil or by coencapsulation of Beauveria bassiana. Formulations containing S. cerevisiae, starch and B. bassiana were attractive for western corn rootworm larvae within the first 4 h following exposure; however, when considering the whole testing period, the maize root systems remained more attractive for the larvae. CONCLUSIONCoencapsulation of S. cerevisiae, starch and B. bassiana is a promising approach for the development of attractive formulations for soil applications. For biological control strategies, the attractiveness needs to be increased by phagostimuli to extend contact between larvae and the entomopathogenic fungus growing out of these formulations. (c) 2016 Society of Chemical Industry C1 [Vemmer, Marina; Patel, Anant V.] Univ Appl Sci, Fac Engn & Math, Fermentat & Formulat Biol & Chem, Interakt 1, D-33619 Bielefeld, Germany. [Schumann, Mario; Vidal, Stefan] Univ Gottingen, Dept Crop Sci, Agr Entomol, Gottingen, Germany. [Beitzen-Heineke, Wilhelm] BIOCARE Gesell Biol Schutzmittel mbH, Einbeck, Germany. [French, Bryan W.] ARS, North Cent Agr Res Lab, USDA, Brookings, SD USA. RP Patel, AV (reprint author), Univ Appl Sci, Fac Engn & Math, Fermentat & Formulat Biol & Chem, Interakt 1, D-33619 Bielefeld, Germany. EM anant.patel@fh-bielefeld.de NR 58 TC 2 Z9 2 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD NOV PY 2016 VL 72 IS 11 BP 2136 EP 2145 DI 10.1002/ps.4245 PG 10 WC Agronomy; Entomology SC Agriculture; Entomology GA DZ2IK UT WOS:000385665300015 PM 26834068 ER PT J AU Fayette, J Raid, R Roberts, PD Jones, JB Pernezny, K Bull, CT Goss, EM AF Fayette, Joubert Raid, Richard Roberts, Pamela D. Jones, Jeffiey B. Pernezny, Ken Bull, Carolee T. Goss, Erica M. TI Multilocus Sequence Typing of Strains of Bacterial Spot of Lettuce Collected in the United States SO PHYTOPATHOLOGY LA English DT Article ID CAMPESTRIS PV.-VITIANS; PATHOGEN PSEUDOMONAS-SYRINGAE; LEAF-SPOT; PLANT PATHOGEN; STATISTICAL TESTS; GENETIC DIVERSITY; DNA POLYMORPHISM; 1ST REPORT; XANTHOMONAS; RECOMBINATION AB Studies on genetic diversity and recombination in bacterial pathogens are providing a better understanding of the mechanisms shaping bacterial diversity, which can affect disease control. Xanthomonas campestris pv. vitians, causal agent of bacterial leaf spot of lettuce, is a threat to the worldwide lettuce industry. We examined the genetic variation within a sample of 83 strains from California, Florida, and Ohio using multilocus sequence typing of six housekeeping genes, totaling 2.7 kb. Additionally, polymorphism in two virulence-related genes, hrpB2 and a putative glycosyl hydrolase, were examined. Based on housekeeping genes, we found three genetic groups of strains that were all able to induce the disease. These included strains collected from weeds and irrigation water that had haplotypes identical to strains from diseased lettuce. High linkage disequilibrium across the sequenced loci indicates that the pathogen is predominantly clonal but recombination has contributed to the observed sequence variation. Although there was significant genetic variation in X. campestris pv. vitians within and among sampled states, identical haplotypes were observed across all three states. This finding suggests that seedborne inoculum may contribute to the diversity of X. campestris pv. vitians in the United States. Knowledge of the genetic structure of the pathogen may be used for developing resistant lettuce varieties. C1 [Fayette, Joubert; Raid, Richard; Pernezny, Ken] Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. [Roberts, Pamela D.] Univ Florida, IFAS, Southwest Florida Res & Educ Ctr, Immokalee, FL USA. [Fayette, Joubert; Raid, Richard; Roberts, Pamela D.; Jones, Jeffiey B.; Pernezny, Ken; Goss, Erica M.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Bull, Carolee T.] USDA ARS, Pacific West Area, 1636 E Alisal St, Salinas, CA USA. [Goss, Erica M.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Bull, Carolee T.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Goss, EM (reprint author), Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA.; Goss, EM (reprint author), Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. EM emgoss@ufl.edu FU University of Florida Institute of Food and Agricultural Sciences Everglades Research and Education Center's Emeritus Faculty Research Endowment FX We thank J. Barak and S. Miller for kindly providing the strains from California and Ohio and anonymous reviewers whose comments improved the manuscript. This research was supported by the University of Florida Institute of Food and Agricultural Sciences Everglades Research and Education Center's Emeritus Faculty Research Endowment. NR 53 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1262 EP 1269 DI 10.1094/PHYTO-11-15-0302-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600003 PM 27359264 ER PT J AU Choudhury, RA Koike, ST Fox, AD Anchieta, A Subbarao, KV Klosterman, SJ McRoberts, N AF Choudhury, R. A. Koike, S. T. Fox, A. D. Anchieta, A. Subbarao, K. V. Klosterman, S. J. McRoberts, N. TI Season-Long Dynamics of Spinach Downy Mildew Determined by Spore Trapping and Disease Incidence SO PHYTOPATHOLOGY LA English DT Article ID PSEUDOPERONOSPORA-CUBENSIS SPORANGIA; BOTRYTIS LEAF-BLIGHT; PERONOSPORA-EFFUSA; AIRBORNE CONIDIA; ERYSIPHE-NECATOR; BREMIA-LACTUCAE; PLANT-DISEASE; REAL-TIME; INFORMATION; PATHOGENS AB Peronospora effusa is an obligate oomycete that causes downy mildew of spinach. Downy mildew threatens sustainable production of fresh market organic spinach in California, and routine fungicide sprays are often necessary for conventional production. In this study, airborne P. effusa spores were collected using rotating arm impaction spore trap samplers at four sites in the Salinas Valley between late January and early June in 2013 and 2014. Levels of P effusa DNA were determined by a species-specific quantitative polymerase chain reaction assay. Peronospora effusa was detected prior to and during the growing season in both years. Nonlinear time series analyses on the data suggested that the within-season dynamics of P. effusa airborne inoculum are characterized by a mixture of chaotic, deterministic, and stochastic features, with successive data points somewhat predictable from the previous values in the series. Analyses of concentrations of airborne P. effusa suggest both an exponential increase in concentration over the course of the season and oscillations around the increasing average value that had season-specific periodicity around 30, 45, and 75 days, values that are close to whole multiples of the combined pathogen latent and infectious periods. Each unit increase in temperature was correlated with 1.7 to 6% increased odds of an increase in DNA copy numbers, while each unit decrease in wind speed was correlated with 4 to 12.7% increased odds of an increase in DNA copy numbers. Disease incidence was correlated with airborne P. effusa levels and weather variables, and a receiver operating characteristic curve analysis suggested that P. effusa DNA copy numbers determined from the spore traps nine days prior to disease rating could predict disease incidence. C1 [Choudhury, R. A.; Subbarao, K. V.; McRoberts, N.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Koike, S. T.] Univ Calif Cooperat Extens, 1432 Abbott St, Salinas, CA 93901 USA. [Fox, A. D.] Fox Weather LLC, Fortuna, CA 95540 USA. [Anchieta, A.; Klosterman, S. J.] USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. RP McRoberts, N (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA.; Klosterman, SJ (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM steve.klosterman@ars.usda.gov; nmcroberts@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU California Leafy Greens Research Program (CLGRP); California Department of Agriculture Specialty Crop block grant program [SCB14043] FX We thank the California Leafy Greens Research Program (CLGRP) and the California Department of Agriculture Specialty Crop block grant program (number SCB14043) for funding this research. We thank W. Mahaffee (USDA-ARS, Corvallis, OR) for providing some spore trap materials and advice and L. Ochoa (USDA-ARS, Salinas, CA) and R. Pena (Hartnell College, Salinas, CA) for collecting spore trap samples. We also thank two anonymous reviewers for their helpful suggestions and advice. NR 44 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1311 EP 1318 DI 10.1094/PHYTO-12-15-0333-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600009 PM 27442537 ER PT J AU Wang, YW Lu, HJ Hu, JG AF Wang, Yunwen Lu, Huangjun Hu, Jinguo TI Molecular Mapping of High Resistance to Bacterial Leaf Spot in Lettuce PI 358001-1 SO PHYTOPATHOLOGY LA English DT Article ID LACTUCA-SATIVA L.; GENETIC-LINKAGE MAP; DISEASE RESISTANCE; DOWNY MILDEW; HIGH-DENSITY; SSR MARKERS; CULTIVARS; ASSOCIATION; INHERITANCE; GERMPLASM AB Lettuce (Lactuca sativa L.) is a diploid (2n = 18) with a genome size of 2,600 Mbp, and belongs to the family Compositae. Bacterial leaf spot (BLS), caused by Xanthomonas campestris pv. vitians, is a major disease of lettuce worldwide. Leaf lettuce PI 358001-1 has been characterized as an accession highly resistant to BLS and has white seed. In order to understand inheritance of the high resistance in this germplasm line, an F-3 population consisting of 163 families was developed from the cross PI 358001-1 x 'Tall Guzmaine' (a susceptible Romaine lettuce variety with black seed). The segregation ratio of reaction to disease by seedling inoculation with X. campestris pv. vitians L7 strain in the F-3 families was shown to be 32:82:48 homozygous resistant/heterozygous/homozygous susceptible, fitting to 1:2:1 (n = 162, chi(2) = 3.19, P = 0.20). The segregation ratio of seed color by checking F-2 plants was 122:41 black/white, fitting to 3:1 (n = 163, chi(2) = 0.002, P = 0.96). The results indicated that both BLS resistance and seed color were inherited as a dominant gene mode. A genetic linkage map based on 124 randomly selected F-2 plants was developed to enable molecular mapping of the BLS resistance and the seed color trait. In total, 199 markers, comprising 176 amplified fragment length polymorphisms, 16 simple-sequence repeats, 5 resistant gene candidate markers, and 2 cleaved amplified polymorphic sequences (CAPS) markers were assigned to six linkage groups. The dominant resistance gene to BLS (Xcvr) was mapped on linkage group 2 and the gene locus y for seed color was identified on linkage group 5. Due to the nature of a single gene inheritance, the high-resistance gene should be readily transferred to adapted lettuce cultivars to battle against the devastating disease of lettuce. C1 [Wang, Yunwen; Lu, Huangjun] Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. [Hu, Jinguo] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. RP Wang, YW; Lu, HJ (reprint author), Univ Florida, Everglades Res & Educ Ctr, Inst Food & Agr Sci, Belle Glade, FL 33430 USA. EM ywwang@ufl.edu; hjlu@ufl.edu FU Florida Specialty Crop Block Grant Program; USDA [00114165]; CRIS [FLA-BGL-005105] FX We thank P. McCord, M. Kanaan, and K. McCorkle at the United States Department of Agriculture (USDA) Agricultural Research Service U.S. Sugar Cane Field Station for providing facilities for DNA quality measurement and sample loading; J. Todd for computer software assistance; and A. Carroll, Ph.D. student R. Kandel, and internship undergraduate student G. Faroutine for lab and greenhouse assistance. This work was supported by grants from Florida Specialty Crop Block Grant Program, USDA grant number 00114165, and the CRIS project number FLA-BGL-005105 to H. Lu. NR 42 TC 0 Z9 0 U1 5 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1319 EP 1325 DI 10.1094/PHYTO-09-15-0238-R PG 7 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600010 PM 27454703 ER PT J AU Klos, KE Gordon, T Bregitzer, P Hayes, P Chen, XM del Blanco, IA Fisk, S Bonman, JM AF Klos, K. Esvelt Gordon, T. Bregitzer, P. Hayes, P. Chen, X. M. del Blanco, I. A. Fisk, S. Bonman, J. M. TI Barley Stripe Rust Resistance QTL: Development and Validation of SNP Markers for Resistance to Puccinia striiformis f. sp hordei SO PHYTOPATHOLOGY LA English DT Article ID NORTH-AMERICA; REGISTRATION; DISEASE; GENES; INHERITANCE; POPULATION; VIRULENCE; STRESS; WHEAT; BURST AB Quantitative trait loci (QTL) for barley stripe rust resistance were mapped in recombinant inbred lines (RIL) from a 'Lenetah' x 'Grannelose Zweizeilige' (GZ) cross. GZ is known for a major seedling resistance QTL on chromosome 4H but linked markers suitable for marker-assisted selection have not been developed. This study identified the 4H QTL (log of the likelihood [LOD] = 15.94 at 97.19 centimorgans [cM]), and additional QTL on chromosomes 4H and 6H (LOD = 5.39 at 72.7 cM and 4.24 at 34.46 cM, respectively). A QTL on chromosome 7H (LOD = 2.04 at 81.07 cM) was suggested. All resistance alleles were derived from GZ. Evaluations of adult plant response in Corvallis, OR in 2013 and 2015 provided evidence of QTL at the same positions. However, the minor QTL on 4H was not statistically significant in either location/year, while the 7H QTL was significant in both. The single-nucleotide polymorphism markers flanking the resistance QTL were validated in RIL from a '95SR316A' x GZ cross for their ability to predict seedling resistance. In 95SR316A x GZ, 91 to 92% of RIL with GZ alleles at the major 4H QTL and at least one other were resistant to moderate in reaction. In these populations, at least two QTL were required to transfer the barley stripe rust resistance from GZ. C1 [Klos, K. Esvelt; Gordon, T.; Bregitzer, P.; Bonman, J. M.] ARS, USDA, Aberdeen, ID 83210 USA. [Hayes, P.; Fisk, S.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Chen, X. M.] USDA ARS, Pullman, WA 99164 USA. [del Blanco, I. A.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Klos, KE (reprint author), ARS, USDA, Aberdeen, ID 83210 USA. EM Kathy.klos@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service [2050-21000-031-00] FX This work was supported by the United States Department of Agriculture Agricultural Research Service, Project Number 2050-21000-031-00. We thank R. Campbell and I. Shakelford for their technical assistance and J. Dubcovsky for his contribution to planning for field evaluations. NR 38 TC 0 Z9 0 U1 18 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1344 EP 1351 DI 10.1094/PHYTO-09-15-0225-R PG 8 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600013 ER PT J AU Nirmala, J Chao, S Olivera, P Babiker, EM Abeyo, B Tadesse, Z Imtiaz, M Talbert, L Blake, NK Akhunov, E Pumphrey, MO Jin, Y Rouse, MN AF Nirmala, Jayaveeramuthu Chao, Shiaoman Olivera, Pablo Babiker, Ebrahiem M. Abeyo, Bekele Tadesse, Zerihun Imtiaz, Muhammad Talbert, Luther Blake, Nancy K. Akhunov, Eduard Pumphrey, Michael O. Jin, Yue Rouse, Matthew N. TI Markers Linked to Wheat Stem Rust Resistance Gene Sr11 Effective to Puccinia graminis f. sp tritici Race TKTTF SO PHYTOPATHOLOGY LA English DT Article ID VIRULENCE; UG99; GERMPLASM; ETHIOPIA; SPREAD; TTKS AB Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant 'Digalu' in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in 'Gabo 56'. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and 'Chinese Spring' exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a 'Berkut/'Scalavatis' population that segregated for Sill, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP_6BL_IWB10724 and ICASP_6BL_IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm. C1 [Nirmala, Jayaveeramuthu; Jin, Yue; Rouse, Matthew N.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res, Fargo, ND 58102 USA. [Olivera, Pablo; Jin, Yue; Rouse, Matthew N.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Babiker, Ebrahiem M.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Abeyo, Bekele] Int Maize & Wheat Improvement Ctr CIMMYT Ethiopia, Addis Ababa, Ethiopia. [Tadesse, Zerihun] Kulumsa Agr Res Ctr, Ethiopian Inst Agr Res, Kulumsa, Ethiopia. [Imtiaz, Muhammad] CIMMYT Pakistan, Islamabad, Pakistan. [Talbert, Luther; Blake, Nancy K.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Pumphrey, Michael O.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Rouse, MN (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA.; Rouse, MN (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM matthew.rouse@ars.usda.gov FU USDA-ARS Appropriated Project [5062-21220-021-00]; USDA-ARS National Plant Disease Recovery System; USDA-ARS Pakistan Wheat Production Enhancement Program; USAID Feed the Future; Durable Rust Resistance in Wheat project; National Research Initiative Competitive Grant (Triticeae-CAP) from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX Funding for this research was provided by USDA-ARS Appropriated Project 5062-21220-021-00, USDA-ARS National Plant Disease Recovery System, USDA-ARS Pakistan Wheat Production Enhancement Program, USAID Feed the Future, the Durable Rust Resistance in Wheat project, and the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. We thank the University of Minnesota Supercomputing Institute for computational support. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other product or vendors that also may be suitable. NR 36 TC 0 Z9 0 U1 10 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1352 EP 1358 DI 10.1094/PHYTO-04-16-0165-R PG 7 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600014 PM 27359266 ER PT J AU Knaus, BJ Tabima, JF Davis, CE Judelson, HS Grunwald, NJ AF Knaus, B. J. Tabima, J. F. Davis, C. E. Judelson, H. S. Grunwald, N. J. TI Genomic Analyses of Dominant US Clonal Lineages of Phytophthora infestans Reveals a Shared Common Ancestry for Clonal Lineages US11 and US18 and a Lack of Recently Shared Ancestry Among All Other US Lineages SO PHYTOPATHOLOGY LA English DT Article ID IRISH POTATO FAMINE; EASTERN UNITED-STATES; DNA-SEQUENCING DATA; OAK DEATH PATHOGEN; POPULATION-STRUCTURE; CENTRAL MEXICO; PHYLOGENETIC ANALYSIS; SEXUAL REPRODUCTION; GENETIC DIVERSITY; PLANT PATHOGEN AB Populations of the potato and tomato late-blight pathogen Phytophthora infestans are well known for emerging as novel clonal lineages. These successions of dominant clones have historically been named US1 through US24, in order of appearance, since their first characterization using molecular markers. Hypothetically, these lineages can emerge through divergence from other U.S. lineages, recombination among lineages, or as novel, independent lineages originating outside the United States. We tested for the presence of phylogenetic relationships among U.S. lineages using a population of 31 whole-genome sequences, including dominant U.S. clonal lineages as well as available samples from global populations. We analyzed ancestry of the whole mitochondrial genome and samples of nuclear loci, including supercontigs 1.1 and 1.5 as well as several previously characterized coding regions. We found support for a shared ancestry among lineages US11 and US18 from the mitochondrial genome as well as from one nuclear haplotype on each supercontig analyzed. The other nuclear haplotype from each sample assorted independently, indicating an independent ancestry. We found no support for emergence of any other of the U.S. lineages from a common ancestor shared with the other U.S. lineages. Each of the U.S. clonal lineages fit a model where populations of new clonal lineages emerge via migration from a source population that is sexual in nature and potentially located in central Mexico or elsewhere. This work provides novel insights into patterns of emergence of clonal lineages in plant pathogen genomes. C1 [Knaus, B. J.; Grunwald, N. J.] USDA ARS, Hort Crop Res Unit, Corvallis, OR 97330 USA. [Tabima, J. F.; Grunwald, N. J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Davis, C. E.; Judelson, H. S.] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crop Res Unit, Corvallis, OR 97330 USA.; Grunwald, NJ (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM Nik.Grunwald@ars.usda.gov OI Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture (USDA) Agricultural Research Service Grant [5358-22000-039-00D]; USDA National Institute of Food and Agriculture Grant [2011-68004-30154] FX We thank three anonymous reviewers and the senior editor for many great suggestions that significantly improved this manuscript; J. Ristaino and B. Fry for sharing previously characterized isolates of U.S. clonal lineages; K. Fairchild, M. Larsen, and C. Press for maintenance of the cultures and general technical support; and the Center for Genome Research and Biocomputing at Oregon State University for access to their high-performance computing facility, genome sequencing, and bioinformatic support. This research is supported, in part, by United States Department of Agriculture (USDA) Agricultural Research Service Grant 5358-22000-039-00D and USDA National Institute of Food and Agriculture Grant 2011-68004-30154 (to N. J. Grunwald and H. S. Judelson). NR 78 TC 1 Z9 1 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1393 EP 1403 DI 10.1094/PHYTO-10-15-0279-R PG 11 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600019 PM 27348344 ER PT J AU Kunjeti, SG Anchieta, A Martin, FN Choi, YJ Thines, M Michelmore, RW Koike, ST Tsuchida, C Mahaffee, W Subbarao, KV Klosteiman, SJ AF Kunjeti, Sridhara G. Anchieta, Amy Martin, Frank N. Choi, Young-Joon Thines, Marco Michelmore, Richard W. Koike, Steven T. Tsuchida, Cayla Mahaffee, Walt Subbarao, Krishna V. Klosteiman, Steven J. TI Detection and Quantification of Bremia lactucae by Spore Trapping and Quantitative PCR SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; LETTUCE DOWNY MILDEW; BOTRYTIS LEAF-BLIGHT; MOLECULAR-DETECTION; AIRBORNE INOCULUM; PHYLOGENETIC-RELATIONSHIPS; PYRENOPEZIZA-BRASSICAE; OILSEED RAPE; PATHOGENS; POPULATIONS AB Bremia lactucae is an obligate, oomycete pathogen of lettuce that causes leaf chlorosis and necrosis and adversely affects marketability. The disease has been managed with a combination of host resistance and fungicide applications with success over the years. Fungicide applications are routinely made under the assumption that inoculum is always present during favorable environmental conditions. This approach often leads to fungicide resistance in B. lactucae populations. Detection and quantification of airborne B. lactucae near lettuce crops provides an estimation of the inoculum load, enabling more judicious timing of fungicide applications. We developed a quantitative polymerase chain reaction (qPCR)-based assay using a target sequence in mitochondrial DNA for specific detection of B. lactucae. Validation using amplicon sequencing of DNA from 83 geographically diverse isolates, representing 14 Bremia spp., confirmed that the primers developed for the TaqMan assays are species specific and only amplify templates from B. lactucae. DNA from a single sporangium could be detected at a quantification cycle (C-q) value of 32, and C-q values >35 were considered to be nonspecific. The coefficient of determination (R-2) for regression between sporangial density derived from flow cytometry and Cq values derived from the qPCR was 0.86. The assay was deployed using spore traps in the Salinas Valley, where nearly half of U.S. lettuce is produced. The deployment of this sensitive B. lactucae-specific assay resulted in the detection of the pathogen during the 2-week lettuce-free period as well as during the cropping season. These results demonstrate that this assay will be useful for quantifying inoculum load in and around the lettuce fields for the purpose of timing fungicide applications based on inoculum load. C1 [Kunjeti, Sridhara G.; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, 1636 E Alisal St, Salinas, CA 93901 USA. [Anchieta, Amy; Martin, Frank N.; Klosteiman, Steven J.] ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. [Choi, Young-Joon] Kunsan Natl Univ, Dept Biol, Gunsan 54150, South Korea. [Choi, Young-Joon; Thines, Marco] Senckenberg Gesellscharft Nat Forsch, Biodivers & Climate Res Ctr BiK F, D-60325 Frankfurt, Germany. [Choi, Young-Joon; Thines, Marco] Goethe Univ Frankfurt, Dept Biol Sci, Inst Ecol Evolut & Divers, D-60325 Frankfurt, Germany. [Michelmore, Richard W.; Tsuchida, Cayla] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Michelmore, Richard W.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA USA. [Tsuchida, Cayla] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Mahaffee, Walt] ARS, USDA, Corvallis, OR USA. RP Klosteiman, SJ (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM Steve.Klosterman@ars.usda.gov OI Subbarao, Krishna/0000-0002-2075-1835 FU California Leafy Greens Research Program; California Department of Food and Agriculture [SCB14043] FX We acknowledge funding from the California Leafy Greens Research Program and the California Department of Food and Agriculture (grant SCB14043). We thank B. McLaughlin and J. E. Van Dyke for expertise and guidance in sorting sporangia using the BD influx instrument at the University of California-Davis Flow Cytometry Shared Resources; R. Pena, L. Ochoa, and F. Wong (USDA-ARS, Salinas, CA) for collecting spore trap samples; A. I. Putman for insightful review of the manuscript; and B. Smith for help in data analysis. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 62 TC 0 Z9 0 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD NOV PY 2016 VL 106 IS 11 BP 1426 EP 1437 DI 10.1094/PHYTO-03-16-0143-R PG 12 WC Plant Sciences SC Plant Sciences GA DZ5KI UT WOS:000385900600022 PM 27392175 ER PT J AU Diaz-Lara, A Martin, RR AF Diaz-Lara, Alfredo Martin, Robert R. TI Blueberry fruit drop-associated virus: A New Member of the Family Caulimoviridae Isolated From Blueberry Exhibiting Fruit-Drop Symptoms SO PLANT DISEASE LA English DT Article ID ROLLING-CIRCLE AMPLIFICATION; PLANT; SEQUENCE; PROTEIN; PARARETROVIRUS; PROMOTER; DATABASE; SEARCH; GENOME AB This study describes the nucleotide sequence and genome organization of a new DNA virus isolated from 'Bluecrop' blueberry plants exhibiting fruit-drop symptoms and named Blueberry fruit drop-associated virus (BFDaV). Blueberry fruit drop disease was first detected in blueberry plants in British Columbia, Canada in the late 1990s, and in a single field in northern Washington state in the United States in 2012. Infected bushes abort nearly 100% of their fruit about three weeks prior to harvest, when the berries are about 3 to 5 mm in diameter. At harvest, the affected plants appear taller than healthy ones as there is no fruit weighing down the branches. The virus was amplified from diseased material using rolling circle amplification, followed by enzyme digestion, cloning, and sequencing. The full genome of BFDaV is 9,850 bp in length and contains a single open reading frame, encoding for a polyprotein, and a large non coding region. Based on the genome size and organization and phylogenetics, BFDaV is proposed as a new and the largest member of family Caulimoviridae. Finally, in mapping part of a field with fruit-drop symptoms, there was a nearly perfect correlation between the presence of the virus and fruit-drop symptoms. C1 [Diaz-Lara, Alfredo] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Martin, Robert R.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Diaz-Lara, A (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM diazlara@oregonstate.edu FU United States Department of Agriculture Agricultural Research Service; Oregon Blueberry Commission; Washington Blueberry Commission; BC Blueberry Industry Development Council; National Clean Plant Network; APHIS Plant Pest and Disease Management and Disaster Prevention Program FX This work was funded by the United States Department of Agriculture Agricultural Research Service, Oregon Blueberry Commission, Washington Blueberry Commission, BC Blueberry Industry Development Council, National Clean Plant Network, and APHIS Plant Pest and Disease Management and Disaster Prevention Program. We thank D. F. Quito-Avila from the Centro de Investigaciones Biotecnologicas del Ecuador-ESPOL (Guayaquil, Ecuador) for helpful discussions. NR 24 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2211 EP 2214 DI 10.1094/PDIS-06-16-0792-RE PG 4 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800004 ER PT J AU Yang, D Zhang, J Wu, MD Chen, WD Li, GQ Yang, L AF Yang, Dan Zhang, Jing Wu, Mingde Chen, Weidong Li, Guoqing Yang, Long TI Characterization of the Mycelial Compatibility Groups and Mating Type Alleles in Populations of Sclerotinia minor in Central China SO PLANT DISEASE LA English DT Article ID FUNGAL PLANT PATHOGEN; GENETIC DIVERSITY; FIELD POPULATIONS; LETTUCE DROP; 1ST REPORT; MARKERS; CANOLA; HOSTS; DIFFERENTIATION; HYPOVIRULENCE AB Ninety-five single-sclerotium isolates were obtained from lettuce and weeds in three counties in central China. They were identified belonging to Sclerotinia minor based on colony morphology and the S. minor-specific DNA marker. Mycelial compatibility groups (MCGs) and the mating type (MAT) alleles in these isolates were determined using the methods of paired cultures and specific PCR, respectively, and the MCG data were used to calculate Shannon's H index (H) and Simpson index (S), thereby evaluating diversity of S. minor. Eight MCGs (MCG1 to MCG8) and two MAT alleles (Inv+, Inv-) were identified in these isolates. Low diversity was detected for the total 95 isolates (H = 1.748, S = 0.786). Isolates of different MCGs or with different MAT alleles did not significantly differ (P > 0.05) in mycelial growth rate on potato dextrose agar (PDA, 20 degrees C) or lesion diameter on lettuce leaves (20 degrees C), but slightly differed in the number of sclerotia produced on PDA (20 degrees C). Furthermore, this study reported five new host plants of S. minor in China, including Capsella bursapastoris, Oenanthe javanica, Fragaria gracilis, Ranunculus ternatus, and Salvia plebeia, and identified three hypovirulent isolates. These results broaden our understanding about the population biology of S. minor. C1 [Yang, Dan; Zhang, Jing; Wu, Mingde; Li, Guoqing; Yang, Long] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Yang, Dan; Zhang, Jing; Wu, Mingde; Li, Guoqing; Yang, Long] Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China. [Chen, Weidong] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. RP Yang, L (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China.; Yang, L (reprint author), Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China. EM yanglong@mail.hzau.edu.cn FU Fundamental Research Funds for the Central Universities [2662015PY040]; National Natural Science Foundation of China [31272084, 31471813]; China Agriculture Research System [CARS-13] FX This study was financially supported by the Fundamental Research Funds for the Central Universities (Grant No. 2662015PY040), the National Natural Science Foundation of China (Grant Nos. 31272084, 31471813), and the earmarked fund for China Agriculture Research System (Grant No. CARS-13). Kind appreciations are due to Dr. Barbara Shew of North Carolina State University for providing S. minor isolates. NR 44 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2313 EP 2318 DI 10.1094/PDIS-12-15-1453-RE PG 6 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800018 ER PT J AU Fraedrich, SW Harrington, TC McDaniel, BA Best, GS AF Fraedrich, S. W. Harrington, T. C. McDaniel, B. A. Best, G. S. TI First Report of Laurel Wilt, Caused by Raffaelea lauricola, on Spicebush (Lindera benzoin) in South Carolina. SO PLANT DISEASE LA English DT News Item ID REDBAY AMBROSIA BEETLE C1 [Fraedrich, S. W.; Best, G. S.] US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Harrington, T. C.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [McDaniel, B. A.] Recreat & Pk Dept, Sumter, SC USA. RP Fraedrich, SW (reprint author), US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. NR 5 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2330 EP 2331 DI 10.1094/PDIS-05-16-0674-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800050 ER PT J AU Malaker, PK Banna, NCD Tiwari, TP Collis, WJ Duveiller, E Singh, PK Joshi, AK Singh, RP Braun, HJ Peterson, GL Pedley, KF Farman, ML Valent, B AF Malaker, P. K. Banna, N. C. D. Tiwari, T. P. Collis, W. J. Duveiller, E. Singh, P. K. Joshi, A. K. Singh, R. P. Braun, H. -J. Peterson, G. L. Pedley, K. F. Farman, M. L. Valent, B. TI First Report of Wheat Blast Caused by Magnaporthe otyzae Pathotype triticum in Bangladesh. SO PLANT DISEASE LA English DT News Item C1 [Malaker, P. K.; Banna, N. C. D.] Bangladesh Agr Res Inst, Wheat Res Ctr, Nashipur, Dinajpur, Bangladesh. [Tiwari, T. P.; Collis, W. J.] House 10B,Rd 53,Gulshan 2,POB 6057, Dhaka 1213, Gulshan, Bangladesh. [Duveiller, E.; Singh, P. K.; Joshi, A. K.; Singh, R. P.; Braun, H. -J.] Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico. [Peterson, G. L.; Pedley, K. F.] ARS, USDA, FDWSRU, Ft Detrick, MD 21702 USA. [Farman, M. L.] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Valent, B.] Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Malaker, PK (reprint author), Bangladesh Agr Res Inst, Wheat Res Ctr, Nashipur, Dinajpur, Bangladesh. NR 2 TC 2 Z9 2 U1 8 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2330 EP 2330 DI 10.1094/PDIS-05-16-0666-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800049 ER PT J AU Olatinwo, R Barton, C Fraedrich, SW Johnson, W Hwang, J AF Olatinwo, R. Barton, C. Fraedrich, S. W. Johnson, W. Hwang, J. TI First Report of Laurel Wilt, Caused by Raffaelea lauricola, on Sassafras (Sassafras albidum) in Arkansas. SO PLANT DISEASE LA English DT News Item ID SOUTHEASTERN UNITED-STATES; CURCULIONIDAE; SCOLYTINAE; COLEOPTERA; DISEASE; REDBAY C1 [Olatinwo, R.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Barton, C.] Arkansas Forestry Commiss, Little Rock, AR 72204 USA. [Fraedrich, S. W.] US Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Johnson, W.; Hwang, J.] US Forest Serv, USDA, Forest Hlth Protect, Pineville, LA 71360 USA. RP Olatinwo, R (reprint author), US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. NR 5 TC 1 Z9 1 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2331 EP 2332 DI 10.1094/PDIS-05-16-0697-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800052 ER PT J AU Fichtner, EJ Browne, GT Mortaz, M Ferguson, L Blomquist, CL AF Fichtner, E. J. Browne, G. T. Mortaz, M. Ferguson, L. Blomquist, C. L. TI First Report of Root Rot Caused by Phytopythium helicoides on Pistachio Rootstock in California. SO PLANT DISEASE LA English DT News Item C1 [Fichtner, E. J.] Univ Calif Cooperat Extens, Tulare Co, Tulare, CA 93274 USA. [Browne, G. T.] Univ Calif Davis, Dept Plant Pathol, USDA ARS, CPGRU, Davis, CA 95616 USA. [Mortaz, M.; Ferguson, L.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Blomquist, C. L.] CDFA, PPDC, Sacramento, CA 95832 USA. RP Fichtner, EJ (reprint author), Univ Calif Cooperat Extens, Tulare Co, Tulare, CA 93274 USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2337 EP 2337 DI 10.1094/PDIS-02-15-1424-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800066 ER PT J AU Tan, SH Talibov, THO Krueger, RR Bodaghi, S Dang, T Chao, YY Greer, G Vidalakis, G AF Tan, S. H. Talibov, T. H. O. Krueger, R. R. Bodaghi, S. Dang, T. Chao, Y. Y. Greer, G. Vidalakis, G. TI First Report of Citrus Exocortis Viroid and Two Citrus Variants of the Hop Stunt Viroid on Lemon in Azerbaijan. SO PLANT DISEASE LA English DT News Item C1 [Tan, S. H.; Bodaghi, S.; Dang, T.; Chao, Y. Y.; Greer, G.; Vidalakis, G.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Talibov, T. H. O.] Bioresources Inst, Nakhichivan Sect, Nakichivan, Azerbaijan. [Krueger, R. R.] ARS, USDA, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. RP Tan, SH (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. NR 5 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD NOV PY 2016 VL 100 IS 11 BP 2341 EP 2341 DI 10.1094/PDIS-05-16-0761-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DZ1OX UT WOS:000385608800076 ER PT J AU Heidari, M Wang, D Delekta, P Sun, SH AF Heidari, Mohammad Wang, Dan Delekta, Phillip Sun, Shuhong TI Marek's disease virus immunosuppression alters host cellular responses and immune gene expression in the skin of infected chickens SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Marek's disease; Immunosuppression; Feather follicle epithelium; Adhesion molecules; CD8 glycoprotein; Cytokines ID IN-VIVO REPLICATION; RIBONUCLEOTIDE REDUCTASE; VACCINATED CHICKENS; VIRULENT MDV; T-CELLS; HERPESVIRUS; IDENTIFICATION; FEATHERS; PCR; TRANSFORMATION AB Marek's disease virus (MDV), a highly cell-associated lymphotropic alpha-herpesvirus, is the causative agent of Marek's disease (MD) in domestic chickens. MDV replicates in chicken lymphocytes and establishes a latent infection within CD4(+) T cells. The latently infected CD4(+) T cells carry the virus to visceral organs, peripheral nerves, and feather follicle epithelium (FFE). FFE is the only anatomical site where infectious enveloped cell-free virus particles are produced and disseminated into the environment. This study investigated the immunological responses and mechanism of viral-induced immunosuppression and immune evasion in the FFE. Strong viral replication and lack of a significant number of cytotoxic T lymphocytes (CTL) in the infected tissues was prominent. Although the overall gene expression pattern was suggestive of a Th1 type immune response, the expression levels of several key immune genes were down regulated in the infected tissues. The mechanism of MDV-induced immunosuppression appears to be through inhibition of CTL function due to down regulation of CD8 glycoprotein and/or blocking of CTL migration due to decrease expression of cell adhesion molecules. Published by Elsevier B.V. C1 [Heidari, Mohammad; Delekta, Phillip] ARS, USDA, Avian Dis & Oncol Lab, 4279 East Mt Hope Rd, E Lansing, MI 48823 USA. [Wang, Dan; Sun, Shuhong] Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Shandong, Peoples R China. RP Heidari, M (reprint author), ARS, USDA, Avian Dis & Oncol Lab, 4279 East Mt Hope Rd, E Lansing, MI 48823 USA. EM mohammad.heidari@ars.usda.gov NR 41 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 1 PY 2016 VL 180 BP 21 EP 28 DI 10.1016/j.vetimm.2016.08.013 PG 8 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DY7SA UT WOS:000385328400004 PM 27692091 ER PT J AU Palmer, MV Thacker, TC Waters, WR AF Palmer, Mitchell V. Thacker, Tyler C. Waters, W. Ray TI Multinucleated giant cell cytokine expression in pulmonary granulomas of cattle experimentally infected with Mycobacterium bovis SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Cytokine; Giant cell; Granuloma; Mycobacterium bovis; RNAScope (R) ID IN-SITU HYBRIDIZATION; GENE-EXPRESSION; TUBERCULOSIS; PROGRESSION; APOPTOSIS; DISEASE; TISSUES; LESIONS; MICE; RNA AB Regardless of host, pathogenic mycobacteria of the Mycobacterium tuberculosis complex such as Mycobacterium bovis, induce a characteristic lesion known as a granuloma, tubercle or tuberculoid granuloma. Granulomas represent a distinct host response to chronic antigenic stimuli, such as foreign bodies, certain bacterial components, or persistent pathogens such as M. bovis. Granulomas are composed of specific cell types including epithelioid macrophages, lymphocytes and a morphologically distinctive cell type, the multinucleated giant cell. Multinucleated giant cells are formed by the fusion of multiple macrophages; however, their function remains unclear. In humans, giant cells in tuberculous granulomas have been shown to express various cytokines, chemokines and enzymes important to the formation and maintenance of the granuloma. The objective of this study was to quantitatively assess multinucleated giant cell cytokine expression in bovine tuberculoid granulomas; focusing on cytokines of suspected relevance to bovine tuberculosis. Using calves experimentally infected with M. bovis, in situ cytokine expression was quantitatively assessed using RNAScope (R) for the following cytokines TNF-alpha, IFN-gamma, TGF-beta, IL-17A and IL-10. Multinucleated giant cells in bovine tuberculoid granulomas expressed all examined cytokines to varying degrees, with differential expression of TGF-beta, IL-17A and IL-10 in giant cells from early versus late stage granulomas. There was a modest, positive correlation between the level of cytokine expression and cell size or number of nuclei. These results suggest that multinucleated giant cells are active participants within bovine tuberculoid granulomas, contributing to the cytokine milieu necessary to form and maintain granulomas. Published by Elsevier B.V. C1 [Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] ARS, Infect Bacterial Dis Livestock Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Palmer, MV (reprint author), Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM mitchell.palmer@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service Project (CRIS) [3625-32000-104] FX The authors thank A. Lasley, S. Zimmerman, H. Trenkamp and the animal care staff at NADC for excellent technical support and animal care. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. Research funds were provided by the US Department of Agriculture, Agricultural Research Service Project (CRIS# 3625-32000-104). NR 38 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD NOV 1 PY 2016 VL 180 BP 34 EP 39 DI 10.1016/j.vetimm.2016.08.015 PG 6 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DY7SA UT WOS:000385328400006 PM 27692093 ER PT J AU Moore, SJ Smith, JD Greenlee, MHW Nicholson, EM Richt, JA Greenlee, JJ AF Moore, S. J. Smith, J. D. Greenlee, M. H. West Nicholson, E. M. Richt, J. A. Greenlee, J. J. TI Comparison of Two US Sheep Scrapie Isolates Supports Identification as Separate Strains SO VETERINARY PATHOLOGY LA English DT Article DE intranasal administration; prion diseases; prion protein; PRNP; PrPSc proteins; scrapie; sheep; transmissible spongiform encephalopathies ID ABNORMAL PRION PROTEIN; GENETICALLY SUSCEPTIBLE SHEEP; CHRONIC WASTING DISEASE; IN-VITRO CONVERSION; NATURAL SCRAPIE; INCUBATION-TIME; SUFFOLK SHEEP; EXPERIMENTAL TRANSMISSION; PRP ACCUMULATION; INFECTED SHEEP AB Scrapie is a naturally occurring transmissible spongiform encephalopathy of sheep and goats. There are different strains of sheep scrapie that are associated with unique molecular, transmission, and phenotype characteristics. However, in the United States, very little is known about the potential presence of scrapie strains. Scrapie strain and PRNP genotype could both affect susceptibility, potential for transmission, incubation period (IP), and control measures required for eliminating scrapie from a flock. The investigators evaluated 2 US scrapie isolates, No. 13-7 and x124, after intranasal inoculation to compare clinical signs, IPs, spongiform lesions, and patterns of PrPSc deposition in sheep with scrapie-susceptible PRNP genotypes (QQ171). After inoculation with x124, susceptibility and IP were associated with valine at codon 136 (V136) of the prion protein: VV136 sheep had short IPs (6.9 months), those in AV136 sheep were 11.9 months, and AA136 sheep did not develop scrapie. All No. 13-7 inoculated sheep developed scrapie, with IPs of 20.1 months for AA136 sheep, 22.8 months for AV136 sheep, and 26.7 months for VV136 sheep. Patterns of immunoreactivity in the brain were influenced by inoculum isolate and host genotype. Differences in PrPSc profiles versus isolate were most striking when examining brains from sheep with the VV136 genotype. Inoculation into C57BL/6 mice resulted in markedly different attack rates (90.5% for x124 and 5.9% for No. 13-7). Taken together, these data demonstrate that No. 13-7 and x124 represent 2 distinct strains of scrapie with different IPs, genotype susceptibilities, and PrPSc deposition profiles. C1 [Moore, S. J.; Smith, J. D.; Nicholson, E. M.; Richt, J. A.; Greenlee, J. J.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Greenlee, M. H. West] Iowa State Univ, Dept Biomed Sci, Coll Vet Med, Ames, IA USA. [Smith, J. D.] Iowa State Univ, Dept Vet Pathol, Coll Vet Med, Ames, IA USA. [Richt, J. A.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. RP Greenlee, JJ (reprint author), ARS, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM justin.greenlee@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This research was funded in its entirety by congressionally appropriated funds to the United States Department of Agriculture, Agricultural Research Service. The funders of the work did not influence study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 0 Z9 0 U1 2 U2 2 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD NOV PY 2016 VL 53 IS 6 BP 1187 EP 1196 DI 10.1177/0300985816629712 PG 10 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA DZ4HQ UT WOS:000385819700011 PM 26936223 ER PT J AU Sun, Y Bai, GY Wang, YX Zhang, YY Pan, J Cheng, WM Feng, XL Li, H Ma, CC Ruan, WB Shapiro-Ilan, DI AF Sun, Yang Bai, Guang-ying Wang, Ying-Xiao Zhang, Yuan-yuan Pan, Jie Cheng, Wei-min Feng, Xiao-ling Li, Hui Ma, Cheng-cang Ruan, Wei-bin Shapiro-Ilan, David I. TI The impact of Cu, Zn and Cr salts on the relationship between insect and plant parasitic nematodes: A reduction in biocontrol efficacy SO APPLIED SOIL ECOLOGY LA English DT Article DE Biocontrol; Meloidogyne incognita; Mortality; Soil health; Beneficial nematode ID HEAVY-METAL CONTAMINATION; ROOT-KNOT NEMATODES; SOIL FOOD-WEB; ENTOMOPATHOGENIC NEMATODES; MELOIDOGYNE-INCOGNITA; HETERORHABDITIS-BACTERIOPHORA; STEINERNEMA-CARPOCAPSAE; LABORATORY CONDITIONS; BIOLOGICAL-CONTROL; CADMIUM ACCUMULATION AB Accumulation and potential health risks of toxic elements are associated with excessive fertilizer and manure applications in vegetable production systems. The goal of the present study was to explore the effects of potential toxic elements on the function of a cohort of beneficial organisms, specifically, the insect parasitic nematodes known as entomopathogenic nematodes. We assessed the impact of heavy metal accumulation on entomopathogenic nematodes (Steinernema carpocapsae and Heterorhabditis bacteriophora) and plant parasitic nematodes (Meloidogyne incognita). Genearlly, pretreatment with 1.5 mg Cu/L, 50 mg Zn/L or 7.5 mg Cr/L solution for 24 h caused direct mortality of infective juvenile S. carpocapsae and H. bacteriophora nematodes, and lowered virulence of H. bacteriophora against Galleria mellonella. Interestingly, we found that 24 h exposure to 4.5 mg Cu/L, 50 mg Zn/L or 7.5 mg Cr/L solution already caused higher mortality in H. bacteriophora than M. incognita in a laboratory assay. Moreover, in a potted plant experiment, pretreatment of H. bacteriophora with Cu, Zn or Cr salts resulted in increased penetration of M. incognita into cucumber roots compared with H. bacteriophora that were not treated (thus reducing biocontrol efficacy). Pretreatment of S. carpocapsae with Cu also caused increased penetration of M. incognita into cucumber roots; however, none of the Zn and Cr pretreatment concentrations exposed to S. carpocapsae resulted in increased M. incognita root penetration, and lower concentrations of these elements caused decreased penetration (enhancing biocontrol efficacy). Our results indicate that heavy metals can have a direct negative effect on entomopathogenic nematodes (via direct mortality) and also an indirect effect by disrupting biocontrol efficacy against plant parasitic nematodes, but in some cases low concentration may stimulate biocontrol activity. High levels of heavy metal accumulation in greenhouse vegetable production systems could substantially influence nematode community structure and impact other soil biota as well. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sun, Yang; Bai, Guang-ying; Wang, Ying-Xiao; Zhang, Yuan-yuan; Feng, Xiao-ling; Ruan, Wei-bin] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. [Pan, Jie] Tianjin Inst Agr Resources & Environm Sci, Tianjin 300192, Peoples R China. [Cheng, Wei-min] Minist Agr, Agroenvironm Protect Inst, Tianjin 300191, Peoples R China. [Li, Hui] Inst Agr Qual Stand & Testing Technol, Tianjin 300381, Peoples R China. [Ma, Cheng-cang] Tianjin Normal Univ, Coll Life Sci, Tianjin 300387, Peoples R China. [Shapiro-Ilan, David I.] USDA ARS, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Ruan, WB (reprint author), Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. EM ruanweibin2004@hotmail.com FU Special Fund for Agroscientific Research in the Public Interest [201103018]; 111 project [B08011]; Natural Science Foundation of China [31170412, 31470495]; Fundamental Research Funds for the Central Universities FX This work was jointly supported by the Special Fund for Agroscientific Research in the Public Interest (201103018) and 111 project (B08011), Natural Science Foundation of China (31170412 and 31470495) and Fundamental Research Funds for the Central Universities. We gratefully acknowledge the anonymous reviewers for their valuable comments on the manuscript. NR 66 TC 0 Z9 0 U1 26 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 108 EP 115 DI 10.1016/j.apsoi1.2016.05.015 PG 8 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400011 ER PT J AU Zhu, SS Vivanco, JM Manter, DK AF Zhu, Shusheng Vivanco, Jorge M. Manter, Daniel K. TI Nitrogen fertilizer rate affects root exudation, the rhizosphere microbiome and nitrogen-use-efficiency of maize SO APPLIED SOIL ECOLOGY LA English DT Article DE Nitrogen-use-efficiency; Maize; Microbial ecology; Fertilizer; N immobilization ID BACTERIAL COMMUNITY STRUCTURE; 16S RIBOSOMAL-RNA; DIAZOTROPHIC BACTERIA; NUTRITIONAL-STATUS; SOIL MICROBIOME; PLANT; DENITRIFICATION; SUGARCANE; SEQUENCES; FIXATION AB The composition and function of microbial communities in the rhizosphere of crops have been linked to edaphic factors and root exudate composition. We examined the effect of N fertilizer (urea) rate on maize root exudation, the associated rhizosphere microbial community, and nitrogen-use-efficiency. Increasing N rate had a significant effect on root exudate quantity and composition. Specifically, the total abundance of sugars, sugar alcohols, and phenolics was positively correlated with N rate (p < 0.005). Similarly, the abundance of rhizosphere bacteria (16 S rRNA copies g(-1) soil FW) was enhanced with increasing N rate. Using PICRUSt, we also explored the metagenomic contribution of bacterial OTUs to the abundance of N cycle-related genes in-the maize rhizosphere. On a relative abundance basis, the nitrifying-(pmoA-amoA) and denitrifying-genes (nirK and nosZ) were significantly influenced by N rate (p <0.05); whereas, the nitrogen fixing (nifD and nifH) arid urease (ureC) genes were not influenced by N rate (p > 0.05). However, on a total abundance basis (gene copies g(-1) soil FW) all N-cycle genes increased significantly with increasing N rate (p < 0.05). Percent N recovery from both soil and fertilizer sources showed a curvilinear response that was highest at intermediate N rates; whereas, fertilizer N lost from the system increased significantly at the two highest N rates (p < 0.05). In summary, our results show high N rates increase both root exudation and the abundance of soil bacteria, which may help explain the decline in fertilizer-use efficiency and loss of N from the system at higher N rates. Published by Elsevier B.V. C1 [Zhu, Shusheng] Yunnan Agr Univ, Key Lab Agrobiodivers & Pest Management, Educ Minist China, Kunming 650201, Yunnan, Peoples R China. [Vivanco, Jorge M.] Colorado State Univ, Deptarment Hort & Landscape Architecture, Ft Collins, CO 80525 USA. [Manter, Daniel K.] ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO 80526 USA. RP Manter, DK (reprint author), ARS, Soil Plant Nutrient Res, USDA, Ft Collins, CO 80526 USA. EM daniel.manter@ars.usda.gov FU National Science Foundation of China [31260447]; "973" program from the Ministry of Science and Technology of China [2011CB100400] FX This study was partially supported by the National Science Foundation of China (31260447) and the "973" program from the Ministry of Science and Technology of China (2011CB100400). NR 69 TC 1 Z9 1 U1 83 U2 83 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 324 EP 333 DI 10.1016/j.apsoi1.2016.07.009 PG 10 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400035 ER PT J AU Bera, T Collins, HP Alva, AK Purakayastha, TJ Patra, AK AF Bera, T. Collins, H. P. Alva, A. K. Purakayastha, T. J. Patra, A. K. TI Biochar and manure effluent effects on soil biochemical properties under corn production SO APPLIED SOIL ECOLOGY LA English DT Article DE Biochar; Manure; Biochemical properties; Soil quality ID MICROBIAL BIOMASS; TEMPERATE SOILS; BLACK CARBON; LOAM SOIL; QUALITY; AMENDMENT; MANAGEMENT; PYROLYSIS; EMISSIONS; PEANUT AB Biochar (BC), an aromatic carbon (C) rich compound has been used to sequester C in terrestrial ecosystems. Biochar has also been shown to improve soil fertility and crop productivity when applied to soil. Biochemical properties of soil are sensitive parameters that indicate the change in soil processes that result from soil management practices. Major knowledge gaps exist on the long term effects of biochar addition in cropped field soils. Therefore, a field experiment (3 yrs) was conducted on a Warden silt loam soil to investigate the changes in soil biochemical properties following three years of corn (Zea mays L.) production with applications of biochar (BC), fertilizer (NPK) or dairy manure (DE). The NPK or DE treatments were applied annually, while BC was applied only the first year. Organic carbon (TOC), total organic nitrogen (TON), TOC to TON ratio (CNR), soil microbial biomass carbon (MBC), Metabolic quotient (MQ), potential mineralizable nitrogen (PMN), fluorescein diacetate hydrolysis activity (FDA), acid phophatase activity (ACP), alkaline phosphatase activity (ALP), beta-glucosidase activity, urease activity (URA), and soil pH were evaluated at 0-15 and 15-30 cm depths after the 3rd year of corn production. A soil quality index equation was also developed with linear scoring functions. NPK or DE with BC application increased soil pH, TOC, MBC, FDA, CN ratio, ALP and URA while decreased MQ and ACP activity. Biochar had little effect on N dynamics in the soil. Application of biochar to both NPK and DE treatments improved the soil quality index as compared to that without biochar. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bera, T.; Collins, H. P.; Alva, A. K.] USDA ARS, Vegetable & Forage Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. [Collins, H. P.] USDA ARS, Grassland Soil & Water Lab, Temple, TX 76502 USA. [Alva, A. K.] USDA ARS, Agr Syst Res Unit, Sidney, MT 59270 USA. [Bera, T.; Purakayastha, T. J.; Patra, A. K.] ICAR Indian Agr Res Inst, Div Soil Sci & Agr Chem, New Delhi 110012, India. [Patra, A. K.] ICAR Indian Inst Soil Sci, Bhopal 462038, MP, India. RP Bera, T (reprint author), USDA ARS, Vegetable & Forage Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM tanumoy.soil@gmail.com FU Fulbright Commission FX This project was conducted as part of the Fulbright-Nehru Doctoral and Professional Research Fellowship to Bera. We gratefully acknowledge the financial support from Fulbright Commission. Bera is also thankful to ICAR and IARI for permitting him to peruse the work in USA during his Ph.D. Bill Boge and Rebeca Cochran are acknowledged for their help in laboratory with the analysis of samples. Finally, we thank the editors and anonymous reviewers of Applied Soil Ecology Journal for their apt comments to improve this publication. NR 63 TC 0 Z9 0 U1 35 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD NOV PY 2016 VL 107 BP 360 EP 367 DI 10.1016/j.apsoil.2016.07.011 PG 8 WC Soil Science SC Agriculture GA DY1MZ UT WOS:000384860400038 ER PT J AU Callaham, MA Snyder, BA James, SW Oberg, ET AF Callaham, Mac A., Jr. Snyder, Bruce A. James, Samuel W. Oberg, Erik T. TI Evidence for ongoing introduction of non-native earthworms in the Washington, DC metropolitan area SO BIOLOGICAL INVASIONS LA English DT Article DE Earthworm invasion; Soil macroinvertebrates; Soil disturbance; Native species conservation; Public land management ID NORTH-AMERICA; EXOTIC EARTHWORMS; BOREAL FORESTS; INVASION; LUMBRICIDAE; OLIGOCHAETA; ACANTHODRILIDAE; MEGASCOLECIDAE; COMPETITION; MOUNTAINS AB Earthworm introductions and invasions are ongoing, with significant consequences for ecological characteristics and function where populations of invasive species reach high densities. In North America the influx of people, goods and materials to coastal cities has long been recognized to be related to introduction and establishment of many different invasive organisms. We conducted surveys for soil invertebrates in the Washington, DC area along the Potomac River corridor to examine the influence of historic soil profile disrupting disturbances on the composition of soil invertebrate communities. Here we report three earthworm taxa that either (1) had never been previously reported in North America (Lumbricidae: Helodrilus oculatus), (2) had never been reported from "wild" caught samples in forested soils (Lumbricidae: Eisenia fetida), or (3) represented a notable range expansion for an introduced species (Lumbricidae: Murchieona muldali). All three species reported here have attributes that give reason for concern over their expansion into North American soils, not least of which is their potential for competitive interactions with the remaining native earthworm species. C1 [Callaham, Mac A., Jr.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Southern Res Stn, Athens, GA 30602 USA. [Snyder, Bruce A.] Kansas State Univ, Div Biol, Ackert Hall, Manhattan, KS 66506 USA. [James, Samuel W.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Oberg, Erik T.] Natl Pk Serv, Yellowstone Natl Pk, WY USA. RP Callaham, MA (reprint author), US Forest Serv, Ctr Forest Disturbance Sci, USDA, Southern Res Stn, Athens, GA 30602 USA. EM mcallaham@fs.fed.us NR 24 TC 0 Z9 1 U1 13 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3133 EP 3136 DI 10.1007/s10530-016-1230-2 PG 4 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900007 ER PT J AU Pinto, SM Ortega, YK AF Pinto, Sarah M. Ortega, Yvette K. TI Native species richness buffers invader impact in undisturbed but not disturbed grassland assemblages SO BIOLOGICAL INVASIONS LA English DT Article DE Diversity; Drought; Exotic plants; Fire; Invasion ID KNAPWEED CENTAUREA-MACULOSA; PLANT DIVERSITY; COMMUNITY INVASIBILITY; PRESCRIBED FIRE; INVASIVE PLANT; BIODIVERSITY; ECOSYSTEMS; PRODUCTIVITY; COMPETITION; MANAGEMENT AB Many systems are prone to both exotic plant invasion and frequent natural disturbances. Native species richness can buffer the effects of invasion or disturbance when imposed in isolation, but it is largely unknown whether richness provides substantial resistance against invader impact in the face of disturbance. We experimentally examined how disturbance (drought/burning) influenced the impact of three exotic invaders (Centaurea stoebe, Linaria dalmatica, or Potentilla recta) on native abundance across a gradient of species richness, using previously constructed grassland assemblages. We found that invaders had higher cover in experimentally disturbed plots than in undisturbed plots across all levels of native species richness. Although exotic species varied in cover, all three invaders had significant impacts on native cover in disturbed plots. Regardless of disturbance, however, invader cover diminished with increasing richness. Invader impacts on native cover also diminished at higher richness levels, but only in undisturbed plots. In disturbed plots, invaders strongly impacted native cover across all richness levels, as disturbance favoured invaders over native species. By examining these ecological processes concurrently, we found that disturbance exacerbated invader impacts on native abundance. Although diversity provided a buffering effect against invader impact without disturbance, the combination of invasion and disturbance markedly depressed native abundance, even in high richness assemblages. C1 [Pinto, Sarah M.] Univ Montana, Dept Organismal Biol & Ecol, Missoula, MT 59812 USA. [Ortega, Yvette K.] US Forest Serv, USDA, Rocky Mt Res Stn, 800 E Beckwith Ave, Missoula, MT 59801 USA. RP Pinto, SM (reprint author), Univ Montana, Dept Organismal Biol & Ecol, Missoula, MT 59812 USA. EM sarahmariannepinto@gmail.com FU National Sciences and Engineering Research Council of Canada (NSERC PGS-D) FX We thank John Maron, Marilyn Marler, Ray Callaway, Dean Pearson, Winsor Lowe, Lisa Eby, and Dan Atwater for their extensive guidance and feedback. We thank many field assistants for their work on this project especially Jeremy Amberson, Katie Holder, Natalie Shapiro, and Crista Clark. We gratefully acknowledge the support of the National Sciences and Engineering Research Council of Canada (NSERC PGS-D). NR 61 TC 0 Z9 0 U1 44 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3193 EP 3204 DI 10.1007/s10530-016-1208-0 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900012 ER PT J AU Porensky, LM Blumenthal, DM AF Porensky, Lauren M. Blumenthal, Dana M. TI Historical wildfires do not promote cheatgrass invasion in a western Great Plains steppe SO BIOLOGICAL INVASIONS LA English DT Article DE Bromus arvensis; Fire regime; Great Basin; Shrub-grass interactions; Thunder Basin National Grassland; Winter precipitation ID BROMUS-TECTORUM INVASION; MIXED-GRASS PRAIRIE; BURNING JAPANESE BROME; CLIMATE-CHANGE; BIOLOGICAL INVASIONS; NORTH-AMERICA; POPULATION-DYNAMICS; TALLGRASS PRAIRIE; PLANT COMMUNITY; SUMMER FIRE AB Plant invasion and wildfire are often tightly linked. Invasive grasses, in particular, can severely alter ecosystems by increasing fire frequency and intensity. In western North America, positive feedbacks between wildfire and Bromus tectorum (cheatgrass) invasion have contributed to widespread plant community conversion. Impacts of conversion include reduced biodiversity, wildlife habitat, and livestock weight gains, as well as increased costs associated with fire-fighting and ecosystem restoration. While B. tectorum has been studied intensively in the Intermountain West, it is unclear whether fire-invasion feedback cycles observed in the Great Basin operate similarly in the western Great Plains, where annual bromes coexist with fire-adapted native species. In a shrub-grass ecotone in northeastern Wyoming, we asked how wildfires have influenced B. tectorum and its congener, B. arvensis, and whether the effects of wildfire on annual bromes varied based on landscape context. We sampled annual bromes along 142 transects associated with 28 historical wildfires (2-26 years since fire). Both brome species were equally likely to occur in burned and unburned sites. Cover of B. tectorum was lower in burned sites. Soil texture, cover of other plant species, slope, and aspect were strongly associated with annual brome abundance. In the western Great Plains, single wildfires do not appear to promote B. tectorum invasion. However, the effects of repeated fires on invasion in this system remain unclear. Our findings stress that relationships between fire and plant invasion are governed not by invader identity alone but by ecosystem-specific interactions among invaders, fire regimes, and resident species. C1 [Porensky, Lauren M.; Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. RP Porensky, LM (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov FU USDA-ARS FX Many thanks to the Thunder Basin Grassland Prairie Ecosystem Association, the US Forest Service Douglas Ranger District, cooperating private land owners, and Justin Derner for facilitating this research. William Armstrong helped to implement the stratified random sampling design. Thanks to Skye Greenler, Megan Gordon, Catherine Estep, Nickolas Dufek and Kevin Mueller for collecting data. David Augustine, Brian Anacker, Elizabeth Leger, Julie Kray, Eugene Schupp, and three anonymous reviewers provided assistance with the manuscript. Funding is from USDA-ARS. NR 69 TC 0 Z9 0 U1 28 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD NOV PY 2016 VL 18 IS 11 BP 3333 EP 3349 DI 10.1007/s10530-016-1225-z PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DY5RN UT WOS:000385159900022 ER PT J AU Renye, JA Somkuti, GA Garabal, JI Steinberg, DH AF Renye, J. A., Jr. Somkuti, G. A. Garabal, J. I. Steinberg, D. H. TI Bacteriocin production by Streptococcus thermophilus in complex growth media SO BIOTECHNOLOGY LETTERS LA English DT Article DE Antimicrobial activity; Bacteriocin; Growth medium; Quorum sensing; Streptococcus thermophilus ID INHIBITORY SPECTRUM; MEDIUM COMPONENTS; SBT1277; PEPTIDE; STRAIN; BOZA AB To test if the production of bacteriocins by Streptococcus thermophilus is influenced when grown in various complex media commonly used for the culturing of lactic acid bacteria. Forty-one strains of S. thermophilus were screened for the production of bacteriocins in tryptone/yeast extract/lactose (TYL), M17-lactose (M17L), M17-glucose (M17G) and MRS media. Two strains, ST144 and ST145, were identified as novel bacteriocin producers, with constitutive production observed only in M17G. Strains ST110, ST114 and ST134 constitutively produced bacteriocins in all growth media but ST114 required growth in MRS for its antimicrobial activity to persist in a 24 h culture. The addition of a synthetic quorum sensing peptide (BlpC) induced bacteriocin production by ST106 in all media tested; and by ST118 in TYL and M17L. Strain ST109, which constitutively produced a bacteriocin in TYL and M17 broths, required BlpC induction when grown in MRS. Real-time PCR analysis showed that the natural expression of blpC in ST109 was lower when grown in MRS, suggesting that something in medium interfered with the blp quorum sensing system. As the choice of growth medium influences both bacteriocin production and peptide stability, several types of production media should be tested when screening for novel bacteriocin-producing strains of S. thermophilus. C1 [Renye, J. A., Jr.; Somkuti, G. A.; Steinberg, D. H.] ARS, Dairy & Funct Food Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Garabal, J. I.] Agr Res Ctr Mabegondo CIAM, Xunta De Galicia 15318, Abegondo, Spain. RP Renye, JA (reprint author), ARS, Dairy & Funct Food Res Unit, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM john.renye@ars.usda.gov NR 24 TC 0 Z9 0 U1 13 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD NOV PY 2016 VL 38 IS 11 BP 1947 EP 1954 DI 10.1007/s10529-016-2184-2 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DY5ZN UT WOS:000385184600015 PM 27515777 ER PT J AU Hubka, V Novakova, A Peterson, SW Frisvad, JC Sklenar, F Matsuzawa, T Kubatova, A Kolarik, M AF Hubka, Vit Novakova, Alena Peterson, Stephen W. Frisvad, Jens C. Sklenar, Frantisek Matsuzawa, Tetsuhiro Kubatova, Alena Kolarik, Miroslav TI A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin-producing species SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Aspergillus nidulans; Codon usage; Emericella; Mycotoxins; Paralogous genes; Subgeneric classification of Aspergillus ID EGYPTIAN DESERT SOIL; INVASIVE ASPERGILLOSIS; EMERICELLA-QUADRILINEATA; TRANSPLANT RECIPIENTS; PHYLOGENETIC ANALYSIS; GENUS ASPERGILLUS; DNA-SEQUENCES; AFLATOXIN B-1; SYNOPTIC KEY; SP NOV. AB Aspergillus section Nidulantes is a speciose group of microscopic fungi whose species are important in indoor air quality, food spoilage, mycotoxin production and human pathogenicity. We assembled as many species from the section as possible with either type specimens or protologues for analysis. DNA sequences were obtained from up to four loci to conduct phylogenetic analysis of sect. Nidulantes and other species in subgenus Nidulantes. Comparisons of phenetic characters were made. Our goal was to recognize monophyletic taxa for section Nidulantes as well as the other taxa in the subgenus. Phylogenetic analysis indicated that sections Versicolores and Aenei formed subclades in the larger clade containing the type species of section Nidulantes. In order to maintain monophyly in section Nidulantes, we synonymized sects. Versicolores and Aenei with sect. Nidulantes. In place of sections, we have identified six subclades in sect. Nidulantes that also have shared phenetic character sets that allow for easier morphological identification of species. Earlier reports showed that standard beta-tubulin primers can occasionally amplify the tubC gene rather than the benA gene they were designed for. We show that codon usage analysis can easily distinguish between these paralogues, and we provide primer pair options to allow benA amplifications. Two new sect. Nidulantes species are described from European caves, A. croceus (Spain) and A. askiburgiensis (Czech Republic). The analysis of exometabolites showed that both species are able to produce the mycotoxin sterigmatocystin and some additional compounds. C1 [Hubka, Vit; Sklenar, Frantisek; Kubatova, Alena; Kolarik, Miroslav] Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic. [Hubka, Vit; Novakova, Alena; Sklenar, Frantisek; Kolarik, Miroslav] Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. [Peterson, Stephen W.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. [Frisvad, Jens C.] Tech Univ Denmark, DTU Syst Biol, Bldg 221, DK-2800 Lyngby, Denmark. [Matsuzawa, Tetsuhiro] Nagasaki Univ, 1-1-1 Manabino, Nagoya, Nagasaki 8512195, Japan. RP Hubka, V (reprint author), Charles Univ Prague, Dept Bot, Fac Sci, Benatska 2, Prague 12801 2, Czech Republic.; Hubka, V (reprint author), Inst Microbiol AS CR, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. EM hubka@biomed.cas.cz RI Kubatova, Alena/D-7918-2016; Kolarik, Miroslav/A-1680-2008 OI Kubatova, Alena/0000-0002-2837-1087; Kolarik, Miroslav/0000-0003-4016-0335 FU Ministry of Education, Youth and Sports of the Czech Republic (SVV project); project "BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University" from the European Regional Development Fund [CZ.1.05/1.1.00/02.0109]; Charles University Grant Agency [GAUK 1130214]; Czech Science Foundation [P506-12-1064] FX This research was supported by the Ministry of Education, Youth and Sports of the Czech Republic (SVV project) and by the project "BIOCEV-Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University" (CZ.1.05/1.1.00/02.0109) from the European Regional Development Fund. Molecular genetic analyses were supported by the projects of Charles University Grant Agency (GAUK 1130214). The survey in European caves was supported through a grant from the Czech Science Foundation (Grant No. P506-12-1064). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. Prof Karol Marhold's consultation on nomenclatural issues is gratefully acknowledged. We thank Dr M. Chudickova and A. Gabrielova, for their invaluable assistance in laboratory, and Dr Z. Jurjevic for valuable comments. NR 123 TC 2 Z9 2 U1 13 U2 13 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 EI 1615-6110 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD NOV PY 2016 VL 302 IS 9 BP 1267 EP 1299 DI 10.1007/s00606-016-1331-5 PG 33 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DY6BX UT WOS:000385192100008 ER PT J AU Stenger, DC Krugner, R Nouri, S Ferriol, I Falk, BW Sisterson, MS AF Stenger, Drake C. Krugner, Rodrigo Nouri, Shahideh Ferriol, Inmaculada Falk, Bryce W. Sisterson, Mark S. TI Sequence polymorphism in an insect RNA virus field population: A snapshot from a single point in space and time reveals stochastic differences among and within individual hosts SO VIROLOGY LA English DT Article DE Virus microevolution; Mutant spectrum; Dicistrovirus ID GLASSY-WINGED SHARPSHOOTER; VESICULAR STOMATITIS-VIRUS; STREAK-MOSAIC-VIRUS; WEST-NILE-VIRUS; HOMALODISCA-VITRIPENNIS; GENETIC-VARIATION; EVOLUTIONARY GENETICS; TEMPORAL DYNAMICS; BLUETONGUE VIRUS; MUTATION AB Population structure of Homalodisca coagulata Virus-1 (HoCV-1) among and within field-collected insects sampled from a single point in space and time was examined. Polymorphism in complete consensus sequences among single-insect isolates was dominated by synonymous substitutions. The mutant spectrum of the C2 helicase region within each single-insect isolate was unique and dominated by nonsynonymous singletons. Bootstrapping was used to correct the within-isolate nonsynonymous:synonymous arithmetic ratio (N:S) for RT-PCR error, yielding an N:S value similar to one log-unit greater than that of consensus sequences. Probability of all possible single-base substitutions for the C2 region predicted N:S values within 95% confidence limits of the corrected within-isolate N:S when the only constraint imposed was viral polymerase error bias for transitions over transversions. These results indicate that bottlenecks coupled with strong negative/purifying selection drive consensus sequences toward neutral sequence space, and that most polymorphism within single-insect isolates is composed of newly-minted mutations sampled prior to selection. Published by Elsevier Inc. C1 [Stenger, Drake C.; Krugner, Rodrigo; Sisterson, Mark S.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Nouri, Shahideh; Ferriol, Inmaculada; Falk, Bryce W.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Stenger, DC (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM drake.stenger@ars.usda.gov FU USDA-ARS [2034-22000-010-00D] FX We thank Kunbo Zhang and Tera L. Pitman for technical support. D.C. Stenger expresses gratitude to Roy French for a decade of insightful discussions on virus evolution that greatly influenced experimental design and analysis of the data presented here. Funding for this research was from USDA-ARS appropriated research project 2034-22000-010-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not constitute endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD NOV PY 2016 VL 498 BP 209 EP 217 DI 10.1016/j.virol.2016.08.026 PG 9 WC Virology SC Virology GA DY1RV UT WOS:000384873000022 PM 27598532 ER PT J AU Mascarin, GM Jaronski, ST AF Mascarin, Gabriel Moura Jaronski, Stefan T. TI The production and uses of Beauveria bassiana as a microbial insecticide SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Review DE Mycoinsecticides; Fermentation; Pests; Formulation; Blastospores; Conidia; White muscardine; Biocontrol ID METARHIZIUM-FLAVOVIRIDE CONIDIA; WEEVIL COSMOPOLITES-SORDIDUS; LYGUS-LINEOLARIS HETEROPTERA; BIOLOGICAL-CONTROL AGENTS; ASIAN LONGHORNED BEETLE; OIL-BASED FORMULATION; ENTOMOPATHOGENIC FUNGI; BEMISIA-TABACI; ENDOPHYTIC COLONIZATION; LIQUID CULTURE AB Among invertebrate fungal pathogens, Beauveria bassiana has assumed a key role in management of numerous arthropod agricultural, veterinary and forestry pests. Beauveria is typically deployed in one or more inundative applications of large numbers of aerial conidia in dry or liquid formulations, in a chemical paradigm. Mass production is mainly practiced by solid-state fermentation to yield hydrophobic aerial conidia, which remain the principal active ingredient of mycoinsecticides. More robust and cost-effective fermentation and formulation downstream platforms are imperative for its overall commercialization by industry. Hence, where economics allow, submerged liquid fermentation provides alternative method to produce effective and stable propagules that can be easily formulated as dry stable preparations. Formulation also continues to be a bottleneck in the development of stable and effective commercial Beauveria-mycoinsecticides in many countries, although good commercial formulations do exist. Future research on improving fermentation and formulation technologies coupled with the selection of multi-stress tolerant and virulent strains is needed to catalyze the widespread acceptance and usefulness of this fungus as a cost-effective mycoinsecticide. The role of Beauveria as one tool among many in integrated pest management, rather than a stand-alone management approach, needs to be better developed across the range of crop systems. Here, we provide an overview of mass-production and formulation strategies, updated list of registered commercial products, major biocontrol programs and ecological aspects affecting the use of Beauveria as a mycoinsecticide. C1 [Mascarin, Gabriel Moura] EMBRAPA Rice & Beans, Rod GO-462,Km 12, BR-75375000 St Antonio De Goias, Go, Brazil. [Jaronski, Stefan T.] ARS, USDA, Pest Management Res Unit, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Mascarin, GM (reprint author), EMBRAPA Rice & Beans, Rod GO-462,Km 12, BR-75375000 St Antonio De Goias, Go, Brazil. EM gmmascar@gmail.com RI Mascarin, Gabriel/E-4646-2015 OI Mascarin, Gabriel/0000-0003-3517-6826 FU EMBRAPA [02.11.07.005.00.00, 02.13.12.003.00.00] FX We thank Mark A. Jackson (USDA, Peoria) and anonymous reviewers for their valuable comments and suggestions. This work was partly supported by EMBRAPA's research Grants No 02.11.07.005.00.00 and 02.13.12.003.00.00. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 158 TC 0 Z9 0 U1 48 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD NOV PY 2016 VL 32 IS 11 AR 177 DI 10.1007/s11274-016-2131-3 PG 26 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DY5NB UT WOS:000385145400004 PM 27628337 ER PT J AU Mills, NJ Beers, EH Shearer, PW Unruh, TR Amarasekare, KG AF Mills, Nicholas J. Beers, Elizabeth H. Shearer, Peter W. Unruh, Thomas R. Amarasekare, Kaushalya G. TI Comparative analysis of pesticide effects on natural enemies in western orchards: A synthesis of laboratory bioassay data SO BIOLOGICAL CONTROL LA English DT Article DE Aphelinus mali; Chrysoperla carnea; Deraeocoris brevis; Galendromus occidentalis; Hippodamia convergens; Trioxys pallidus ID ECOLOGICAL RISK-ASSESSMENT; TABLE RESPONSE EXPERIMENTS; POPULATION-GROWTH RATE; PEST-MANAGEMENT; LADY BEETLE; LAMBDA-CYHALOTHRIN; BIOLOGICAL-CONTROL; BIOCONTROL AGENTS; INSECTICIDES; EXPOSURE AB Pesticides are commonly used for pest management in apple, pear and walnut orchards in the western U.S. and may disrupt biological control of secondary pests in these crops. A comparative analysis was made of results obtained from a series of laboratory bioassays of acute mortality and life table response experiments to estimate lethal and sublethal effects of eight pesticides on seven natural enemy species through use of stage-structured population models. Even though a number of the pesticides tested were reduced-risk products, all of them with the exception of copper plus mancozeb and chlorantraniliprole, caused more than 80% acute mortality of at least one life stage of at least one of the natural enemy species at a full field-rate concentration and could thus be considered moderately harmful according to the International Organization for Biological Control classification for laboratory bioassays. Important sublethal effects included reductions in daily fecundity and egg fertility. From integration of the lethal and sublethal effects in matrix models, the mean of the estimated intrinsic rates of increase for natural enemy species was negative for exposure to cyantraniliprole, lambda-cyhalothrin and spinetoram, but positive and not significantly different from the control for exposure to chlorantraniliprole, copper plus mancozeb, novaluron, and sulfur. For comparisons among pesticides, there appears to be considerable variation in response among natural enemy species that can only be represented effectively from a full life table response experiment and a population-level endpoint, whereas among natural enemy species, their population-level response to the range of pesticides tested could frequently be represented by acute adult mortality alone. (C) 2016 Elsevier Inc. All rights reserved. C1 [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Mulford Hall, Berkeley, CA 94720 USA. [Beers, Elizabeth H.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Unruh, Thomas R.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Mills, NJ (reprint author), Univ Calif Berkeley, Dept Environm Sci & Policy Management, Mulford Hall, Berkeley, CA 94720 USA. EM nmills@berkeley.edu FU USDA-SCRI grant [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board; pear growers in Hood River (Oregon) FX This research was supported by USDA-SCRI grant 2008-04854 and matching funds from the Washington Tree Fruit Research Commission, California Walnut Board and pear growers in Hood River (Oregon). We thank DuPont Crop Protection, Dow Agro Sciences LLC, Chemtura AgroSolutions, Syngenta LLC and Micro Flo Company LLC for providing the pesticides used for this study. The research personnel and technical support of the laboratories from the University of California at Berkeley, Oregon State University, Washington State University and USDA-ARS Yakima are gratefully acknowledged. NR 56 TC 11 Z9 11 U1 25 U2 25 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 17 EP 25 DI 10.1016/j.biocontrol.2015.05.006 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200003 ER PT J AU Bigot-Clivot, A Ladeiro, MP Lepoutre, A Bastien, F Bonnard, I Dubey, JP Villena, I Aubert, D Geffard, O Francois, A Geffard, A AF Bigot-Clivot, Aurelie Ladeiro, Melissa Palos Lepoutre, Alexandra Bastien, Fanny Bonnard, Isabelle Dubey, Jitender P. Villena, Isabelle Aubert, Dominique Geffard, Olivier Francois, Adeline Geffard, Alain TI Bioaccumulation of Toxoplasma and Cryptosporidium by the freshwater crustacean Gammarus fossarum: Involvement in biomonitoring surveys and trophic transfer SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Protozoa; Oocysts; Gammarids; Molecular detection ID MUSSELS DREISSENA-POLYMORPHA; OYSTERS CRASSOSTREA-VIRGINICA; DIGESTIVE ENZYME-ACTIVITIES; POLYMERASE-CHAIN-REACTION; CLAMS CORBICULA-FLUMINEA; MARINE BIVALVE SHELLFISH; ENHYDRA-LUTRIS-NEREIS; LONG-TERM SURVIVAL; REAL-TIME PCR; GONDII OOCYSTS AB The protozoa Toxoplasma gondii and Cryptosporidium parvum are public health priorities because their oocysts can persist in recreational, surface, drinking, river, and sea water sources for a long time. To evaluate the capacity of the freshwater crustacean Gammarus fossarum to accumulate T. gondii and C. parvum oocysts, gammarids were exposed to 200, 2000 or 20,000 oocysts per gammarid and per day for 21 days followed by 5 days of depuration. C parvum DNA was detected by qPCR in G. fossarum in only one out of four pools for the highest concentration and after 14 days of exposure, and T. gondii DNA was detected after 7 days of exposure to the two highest concentrations. Our results document the capacity of G. fossarum to accumulate T gondii in its tissues proportionally to the ambient concentration; the maximum number of oocysts was detected in gammarid tissues after exposure to 20,000 oocysts per day. Mean values of 3.26 ( +/- 3), 21.71 ( +/- 15.18), and 17.41 ( +/- 10.89) oocysts were detected in gammarids after 7, 14, and 21 days, respectively, and after 5 days of depuration, I gondii oocysts were still present in gammarid tissues. These results show for the first time that a freshwater crustacean can bioaccumulate T. gondii oocysts, suggesting that G. fossarum is a potential effective bioindicator of protozoan contamination in biomonitoring studies. Moreover, due to its key position in freshwater food webs, G. fossarum could also play a role in the trophic transfer of protozoa. (C) 2016 Elsevier Inc. All rights reserved. C1 [Bigot-Clivot, Aurelie; Ladeiro, Melissa Palos; Lepoutre, Alexandra; Bastien, Fanny; Bonnard, Isabelle; Geffard, Alain] Univ Reims, Unite Stress Environm & BIOSurveillance Milieux A, UMR SEBIO I 02, Reims, France. [Villena, Isabelle; Aubert, Dominique] Hop Maison Blanche Reims, SFR CAP Sante FED 4231, EA 3800, Lab Parasitol Mycol, Reims, France. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Geffard, Olivier; Francois, Adeline] Inst Natl Rech Sci & Technol Environm & Agr, Unite Rech Milieux Aquat Ecol & Pollut, Ecotoxicol, Villeurbanne, France. RP Bigot-Clivot, A (reprint author), Univ Reims, Unite Stress Environm & BIOSurveillance Milieux A, UMR SEBIO I 02, Reims, France. EM aurelie.bigot@univ-reims.fr FU Agence nationale de securite sanitaire de l'alimentation, de l'environnement et du travail [2014/1/009 INQUASAN] FX The study was supported by the "Agence nationale de securite sanitaire de l'alimentation, de l'environnement et du travail" (projet no 2014/1/009 INQUASAN). NR 50 TC 0 Z9 0 U1 12 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD NOV PY 2016 VL 133 BP 188 EP 194 DI 10.1016/j.ecoenv.2016.07.006 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DX5DA UT WOS:000384398800021 PM 27454203 ER PT J AU Ponsankar, A Vasantha-Srinivasan, P Senthil-Nathan, S Thanigaivel, A Edwin, ES Selin-Rani, S Kalaivani, K Hunter, WB Alessandro, RT Abdel-Megeed, A Paik, CH Duraipandiyan, V Al-Dhabi, NA AF Ponsankar, Athirstam Vasantha-Srinivasan, Prabhakaran Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Edwin, Edward-Sam Selin-Rani, Selvaraj Kalaivani, Kandaswamy Hunter, Wayne B. Alessandro, Rocco T. Abdel-Megeed, Ahmed Paik, Chae-Hoon Duraipandiyan, Veeramuthu Al-Dhabi, Naif Abdullah TI Target and non-target toxicity of botanical insecticide derived from Couroupita guianensis L. flower against generalist herbivore, Spodoptera litura Fab. and an earthworm, Eisenia foetida Savigny SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Cannonball flower; Tropical armyworm secondary metabolites; Octacosane larval mortality; Food consumption; Earthworm; Histology; Longevity ID ANOPHELES-STEPHENSI; LITTORALIS LEP.; ESSENTIAL OIL; NOCTUIDAE; ANTIFEEDANT; EXTRACTS; GROWTH; LARVAE; LEPIDOPTERA; HUBNER AB Botanical insecticides may provide alternatives to synthetic insecticides for controlling Spodoptera litura (F.) and they are target specific, biodegradable, and harmless to mammals. Eight natural chemical compounds with larvicidal activity were identified from fraction F-6 of C. guianensis flower extract Probit analysis of 95% confidence level exposed an LC50 of 223 ppm against S. litura third instar larvae. The growth and development of S. titura was affected in sub-lethal concentrations of fraction F-6 (50, 100, 150 and 200 ppm) compared to controls. Similarly nutritional indices values decreased significantly compared to controls. Fraction F-6 also damaged the gut epithelial layer and brush border membrane (BBM). This study also resolved the effects of toxicity to non-target earthworm treated with fraction F-6 and chemical pesticides (monotrophos and cypermethrin) and the results showed that fraction F-6 had no harmful effect on E. fetida. Further, fraction F-6 was eluted and sub fractions F-6c (50 ppm) showed high mortality against S. litura third instar larvae. Octacosane from fraction F-6c was established and confirmed using IR spectrum and HPLC. The time of retention of fraction F-6c was confirmed with the octacosane standard. Fraction F-6 of C guianensis extract caused dose-dependent mortality towards S. litura Octacosane in fraction F-6c was establish to be the prominent chemical compound associated with causing mortality but other compounds present in the fraction F-6 were shown to be associated with changes in development of S. litura at low dosages. S. litura at low dosage. Therefore, these findings suggest that octacosane may be one of the major insecticidal compounds affecting S. litura survival. (C) 2016 Elsevier Inc. All rights reserved. C1 [Ponsankar, Athirstam; Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Edwin, Edward-Sam; Selin-Rani, Selvaraj] Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Post Grad & Res Dept Zool, Tirunelveli 627802, Tamil Nadu, India. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Alessandro, Rocco T.] Treasure Coast Chem Consultants LLC, 107 Lakes End Dr,Apt B, Ft Pierce, FL 34982 USA. [Abdel-Megeed, Ahmed] Univ Alexandria, Fac Agr, Dept Plant Protect, POB 21531, Alexandria 21526, Egypt. [Paik, Chae-Hoon] Natl Inst Crop Sci, Planning & Coordinat Div, 181 Hyeoksin Ro, Wanju Gun 565851, Jeollabuk Do, South Korea. [Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Environm Studies, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in RI Veeramuthu, Duraipandiyan/D-9924-2012; OI Veeramuthu, Duraipandiyan/0000-0002-2107-3503; Abdel-Megeed, Ahmed/0000-0002-9777-4808 FU King Saud University FX This research was full financially supported by the King Saud University, through Vice Deanship of Research Chairs. NR 54 TC 0 Z9 0 U1 10 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD NOV PY 2016 VL 133 BP 260 EP 270 DI 10.1016/j.ecoenv.2016.06.043 PG 11 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DX5DA UT WOS:000384398800030 PM 27476000 ER PT J AU Maynard, JJ Karl, JW Browning, DM AF Maynard, Jonathan J. Karl, Jason W. Browning, Dawn M. TI Effect of spatial image support in detecting long-term vegetation change from satellite time-series SO LANDSCAPE ECOLOGY LA English DT Article DE Time series; MODIS; Landsat; Image support; Arid ecosystems; Breaks for additive season and trend ID LANDSAT SURFACE REFLECTANCE; STRUCTURAL-CHANGE MODELS; FIRE RISK-ASSESSMENT; REMOTE-SENSING DATA; HERBACEOUS BIOMASS; NATIONAL-PARK; NOAA-AVHRR; ECOSYSTEM; NDVI; RANGELANDS AB Arid rangelands have been severely degraded over the past century. Multi-temporal remote sensing techniques are ideally suited to detect significant changes in ecosystem state; however, considerable uncertainty exists regarding the effects of changing image resolution on their ability to detect ecologically meaningful change from satellite time-series. (1) Assess the effects of image resolution in detecting landscape spatial heterogeneity. (2) Compare and evaluate the efficacy of coarse (MODIS) and moderate (Landsat) resolution satellite time-series for detecting ecosystem change. Using long-term (similar to 12 year) vegetation monitoring data from grassland and shrubland sites in southern New Mexico, USA, we evaluated the effects of changing image support using MODIS (250-m) and Landsat (30-m) time-series in modeling and detecting significant changes in vegetation using time-series decomposition techniques. Within our study ecosystem, landscape-scale (> 20-m) spatial heterogeneity was low, resulting in a similar ability to detect vegetation changes across both satellite sensors and levels of spatial image support. While both Landsat and MODIS imagery were effective in modeling temporal dynamics in vegetation structure and composition, MODIS was more strongly correlated to biomass due to its cleaner (i.e., fewer artifacts/data gaps) 16-day temporal signal. The optimization of spatial/temporal scale is critical in ensuring adequate detection of change. While the results presented in this study are likely specific to arid shrub-grassland ecosystems, the approach presented here is generally applicable. Future analysis is needed in other ecosystems to assess how scaling relationships will change under different vegetation communities that range in their degree of landscape heterogeneity. C1 [Maynard, Jonathan J.; Karl, Jason W.; Browning, Dawn M.] New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER, POB 30003, Las Cruces, NM 88003 USA. RP Maynard, JJ (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER, POB 30003, Las Cruces, NM 88003 USA. EM jmaynard@nmsu.edu NR 48 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2045 EP 2062 DI 10.1007/s10980-016-0381-y PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000010 ER PT J AU Levi, MR Bestelmeyer, BT AF Levi, Matthew R. Bestelmeyer, Brandon T. TI Biophysical influences on the spatial distribution of fire in the desert grassland region of the southwestern USA SO LANDSCAPE ECOLOGY LA English DT Article DE Fire probability; Ecological sites; Soil properties; Desert grasslands; Chihuahuan Desert; Madrean Archipelago; Multi-model inference ID WESTERN UNITED-STATES; NORTHERN CHIHUAHUAN DESERT; VEGETATION DYNAMICS; WILDFIRE IGNITIONS; PRESCRIBED FIRE; SONORAN DESERT; MOJAVE DESERT; CLIMATE; RESTORATION; MODELS AB Fire is an important driver of ecological processes in semiarid systems and serves a vital role in shrub-grass interactions. In desert grasslands of the southwestern US, the loss of fire has been implicated as a primary cause of shrub encroachment. Where fires can currently be re-introduced given past state changes and recent restoration actions, however, is unknown and controversial. Our objective was to evaluate the interactive effects of climate, urban development, and topo-edaphic properties on fire distribution in the desert grassland region of the southwestern United States. We characterized the spatial distribution of fire in the Chihuahuan Desert and Madrean Archipelago ecoregions and investigated the influence of soil properties and ecological site groups compared to other commonly used biophysical variables using multi-model inference. Soil-landscape properties significantly influenced the spatial distribution of fire ignitions. Fine-textured bottomland ecological site classes experienced more fires than expected in contrast to upland sites with coarse soil textures and high fragment content that experienced fewer fire ignitions than expected. Influences of mean annual precipitation, distance to road/rail, soil available water holding capacity (AWHC) and topographic variables varied between ecoregions and political jurisdictions and by fire season. AWHC explained more variability of fire ignitions in the Madrean Archipelago compared to the Chihuahuan Desert. Understanding the spatiotemporal distribution of recent fires in desert grasslands is needed to manage fire and predict responses to climate change. The use of landscape units such as ecological sites presents an opportunity to improve predictions at management scales. C1 [Levi, Matthew R.; Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER, Box 30003, Las Cruces, NM 88003 USA. RP Levi, MR (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER, Box 30003, Las Cruces, NM 88003 USA. EM mrlevi21@nmsu.edu FU USDA ARS Postdoctoral Research Associate Program; NSF Long-Term Ecological Research Program [DEB0080412] FX This work was supported by the USDA ARS Postdoctoral Research Associate Program and the NSF Long-Term Ecological Research Program (DEB0080412). We would like to acknowledge C. Stripling for sharing data from the Texas Forest Service database, S. Schrader for help with collecting and processing spatial data for environmental covariates, B. Cade for suggestions regarding model averaging and D. James for help with R code. NR 58 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2079 EP 2095 DI 10.1007/s10980-016-0383-9 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000012 ER PT J AU Prasad, AM Iverson, LR Matthews, SN Peters, MP AF Prasad, Anantha M. Iverson, Louis R. Matthews, Stephen N. Peters, Matthew P. TI A multistage decision support framework to guide tree species management under climate change via habitat suitability and colonization models, and a knowledge-based scoring system SO LANDSCAPE ECOLOGY LA English DT Article DE Decision support system; Tree habitat suitability model; Tree species migration model; Tree species distribution model; Forest management; Climate change ID EASTERN UNITED-STATES; RANGE SHIFTS; SEED DISPERSAL; GLOBAL CHANGE; POPULATION BIOLOGY; SCALE; DISTRIBUTIONS; UNCERTAINTY; CHALLENGES; FORESTS AB No single model can capture the complex species range dynamics under changing climates-hence the need for a combination approach that addresses management concerns. A multistage approach is illustrated to manage forested landscapes under climate change. We combine a tree species habitat model-DISTRIB II, a species colonization model-SHIFT, and knowledge-based scoring system-MODFACs, to illustrate a decision support framework. Using shortleaf pine (Pinus echinata) and sugar maple (Acer saccharum) as examples, we project suitable habitats under two future climate change scenarios (harsh, Hadley RCP8.5 and mild CCSM RCP4.5 at similar to 2100) at a resolution of 10 km and assess the colonization likelihood of the projected suitable habitats at a 1 km resolution; and score biological and disturbance factors for interpreting modeled outcomes. Shortleaf pine shows increased habitat northward by 2100, especially under the harsh scenario of climate change, and with higher possibility of natural migration confined to a narrow region close to the current species range boundary. Sugar maple shows decreased habitat and has negligible possibility of migration within the US due to a large portion of its range being north of the US border. Combination of suitable habitats with colonization likelihoods also allows for identification of potential locations appropriate for assisted migration, should that be deemed feasible. The combination of these multiple components using diverse approaches leads to tools and products that may help managers make management decisions in the face of a changing climate. C1 [Prasad, Anantha M.; Iverson, Louis R.; Peters, Matthew P.] USDA Forest Serv, Northern Res Stn, 359 Main Rd, Delaware, OH 43015 USA. [Matthews, Stephen N.] Ohio State Univ, Sch Environm & Nat Resources, 2021 Coffey Rd, Columbus, OH 43210 USA. RP Prasad, AM (reprint author), USDA Forest Serv, Northern Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM aprasad@fs.fed.us RI Matthews, Stephen/D-1050-2012 FU Northern Research Station, USDA Forest Service FX Thanks to the Northern Research Station, USDA Forest Service, for funding, and external reviewers Maria Janowiak and Laura Leites for their help in improving the manuscript. We declare no conflict of interest to the best of our knowledge. NR 71 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD NOV PY 2016 VL 31 IS 9 BP 2187 EP 2204 DI 10.1007/s10980-016-0369-7 PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DX5RB UT WOS:000384438000019 ER PT J AU Proestou, DA Vinyard, BT Corbett, RJ Piesz, J Allen, SK Small, JM Li, C Liu, M DeBrosse, G Guo, XM Rawson, P Gomez-Chiarri, M AF Proestou, Dina A. Vinyard, Bryan T. Corbett, Ryan J. Piesz, Jessica Allen, Standish K., Jr. Small, Jessica M. Li, Cui Liu, Ming DeBrosse, Gregory Guo, Ximing Rawson, Paul Gomez-Chiarri, Marta TI Performance of selectively-bred lines of eastern oyster, Crassostrea virginica, across eastern US estuaries SO AQUACULTURE LA English DT Article DE Crassostrea virginica; Performance; Selection; G x E interaction; Disease; Aquaculture; Oyster; Breeding ID HAPLOSPORIDIUM-NELSONI MSX; QUANTITATIVE PCR ASSAY; ENVIRONMENT INTERACTIONS; DISEASE RESISTANCE; PERKINSUS-MARINUS; DERMO DISEASE; DELAWARE BAY; MORTALITY; GROWTH; SURVIVAL AB Eastern oyster, Crassostrea virginica, aquaculture has expanded greatly in recent years, but further growth of the industry is constrained by disease-related losses. Oyster breeding programs supporting the oyster aquaculture industry along the east coast of the US have targeted resistance to three prominent diseases: MSX, Dermo, and ROD, caused by Haplosporidium nelsoni, Perkinsus marinus, and Roseovarius crassostreae respectively. Consequently, selected oyster lines possess some level of resistance and/or tolerance but the extent to which these lines, derived from various programs, perform across diverse growing environments used by industry has not been tested. The performance of six selected eastern oyster lines was evaluated at five sites along the east coast of the US (Maine to Virginia) to 1) identify differences in performance among lines at each site, and 2) identify lines that perform well across all sites. Performance measures included growth, mortality, and yield over a 15-month evaluation period. During unusually high mortality events, subsets of oysters were processed for disease diagnosis. Growth trajectories were similar among lines within a site, but varied significantly across sites (78% of random variance explained). Oysters grown in Rhode Island were largest while oysters grown in Maine were smallest at the end of the study. Mortality varied greatly among lines at each site as well as among sites. Line x site interaction explained 61% of the total random variance in the mortality data. In Maine, extensive mortality was observed early in the year for all lines, coincident with increased ROD prevalence. In New Jersey and Virginia, unusually high mortality was evident in the UMFS, Clinton, and NEH-RI lines during the final months of the experiment when the prevalence of both Dermo and MSX were 100% and <50%, respectively. NEH, DEBY, and hANA lines were less affected, demonstrating that lines selected to perform better in their native site surpassed those selected outside the area. Despite large and significant line x site interaction effects for mortality and yield, NEH, DEBY, and hANA performance was above average across all sites. These findings have important implications for oyster breeding strategies and industry practices. Statement of relevance: First to evaluate multiple oyster lines across diverse sites. Published by Elsevier B.V. C1 [Proestou, Dina A.] ARS, USDA, Natl Cold Water Marine Aquaculture Ctr, 469 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. [Vinyard, Bryan T.] ARS, USDA, Northeast Area Stat Grp, Bldg 005,Room 126,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Corbett, Ryan J.; Piesz, Jessica; Gomez-Chiarri, Marta] Dept Fisheries Anim & Vet Sci, 170 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. [Allen, Standish K., Jr.; Small, Jessica M.] Coll William & Mary, Virginia Inst Marine Sci, Rt 1208 Greate Rd, Gloucester Point, VA 23062 USA. [Li, Cui; Liu, Ming; DeBrosse, Gregory; Guo, Ximing] Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, 6959 Miller Ave, Port Norris, NJ 08349 USA. [Rawson, Paul] Univ Maine, 5751 Murray Hall, Orono, ME 04469 USA. RP Proestou, DA (reprint author), ARS, USDA, Natl Cold Water Marine Aquaculture Ctr, 469 CBLS,120 Flagg Rd, Kingston, RI 02881 USA. EM dina.proestou@ars.usda.gov; bryan.vinyard@ars.usda.gov; corbett214@uri.edu; piesz.jessica@gmail.com; ska@vims.edu; jamoss@vims.edu; cuili@hsrl.rutgers; mliu@hsrl.rutgers.edu; debrosse@rci.rutgers.edu; xguo@hsrl.rutgers.edu; prawson@maine.edu; gomezchi@uri.edu FU USDA ARS CRIS project [80303100000300D] FX The authors thank Chris Davis (Pequamid Oyster Co.), Russell Blank (Rome Point Oysters), and Rob Krause (Ninigret Oyster Farm) for providing space at their aquaculture leases in ME, RIBay, and RIPond. Additional assistance was provided by hatchery and field crews at the University of Maine and the Virginia Institute of Marine Science, and several undergraduate students at the University of Rhode Island. Funding for this project was obtained through the USDA ARS CRIS project # 80303100000300D. NR 56 TC 1 Z9 1 U1 20 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 17 EP 27 DI 10.1016/j.aquaculture.2016.06.012 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300003 ER PT J AU Evenhuis, JP Mohammed, H LaPatra, SE Welch, TJ Arias, CR AF Evenhuis, Jason P. Mohammed, Haitham LaPatra, Scott E. Welch, Timothy J. Arias, Covadonga R. TI Virulence and molecular variation of Flavobacterium columnare affecting rainbow trout in Idaho, USA SO AQUACULTURE LA English DT Article DE Flavobacterium columnare; Genomovar I; AFLP; Bacterial challenge ID CATFISH ICTALURUS-PUNCTATUS; FLEXIBACTER-COLUMNARIS; ONCORHYNCHUS-MYKISS; FISH; GENOMOVAR; ASSOCIATION AB Columnaris disease, caused by Flavobacterium columnare, is an emerging problem in the rainbow trout (Oncorhynchus mykiss) aquaculture industry of Idaho. All F. columnare isolates taken from disease outbreaks in the rain bow trout producing region of southern Idaho, and for rainbow trout, are all genomovar I. Virulence phenotyping of 11 genomovar I and 1 genomovar I/II isolates, taken from 6 different farms, suggests significant variation in virulence toward rainbow trout with the most virulent strains having an LD50 of 1 x 10(6) CFU/ml(-1) when using a waterborne challengemodel. The least virulent strain required 8 x 10(6) CFU/ml(-1) to reach the LD50. AFLP fingerprinting of these strains and the resulting phylogenetic tree show that all of the strains tested had a percent similarity of 75% or higher, save one, the MS-FC-4 strain had <60% identity to all of the other strains tested. These results suggest strains of F. columnare isolated from disease outbreaks in rainbow trout production have a higher degree of diversity than previously thought. Published by Elsevier B.V. C1 [Evenhuis, Jason P.; Welch, Timothy J.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [Mohammed, Haitham] Assiut Univ, Dept Anim Med, Fac Vet Med, Assiut 71526, Egypt. [LaPatra, Scott E.] Clear Springs Foods Inc, Div Res, Buhl, ID 83316 USA. [Arias, Covadonga R.] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, 203 Swingle Hall, Auburn, AL 36849 USA. RP Evenhuis, JP (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM jason.evenhuis@ars.usda.gov FU USDA/ARS CRIS [8082-32000-006] FX The authors thank Ryan Lipscomb, Clayton Birkett, James Kincaid and Travis Moreland for expert technical assistance, and Carol A. Evenhuis for critical comments on the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. This work was supported by USDA/ARS CRIS 8082-32000-006, USDA is an equal opportunity provider and employer. NR 23 TC 1 Z9 1 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 106 EP 110 DI 10.1016/j.aquaculture.2016.06.017 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300014 ER PT J AU Schroeter, JC Peterson, BC Small, BC AF Schroeter, Julie C. Peterson, Brian C. Small, Brian C. TI Development of a multitissue microfluidic array for assessing changes in gene expression associated with channel catfish appetite, growth, metabolism, and intestinal health SO AQUACULTURE LA English DT Article DE Channel catfish; Gene expression; Array; Microfluidic ID COMPLEMENTARY DEOXYRIBONUCLEIC-ACID; ORANGE-SPOTTED GROUPER; FOOD-INTAKE; NEUROPEPTIDE-Y; MESSENGER-RNA; MOLECULAR CHARACTERIZATION; EPINEPHELUS-COIOIDES; ICTALURUS-PUNCTATUS; HORMONE-SECRETION; SPARUS-AURATA AB Large-scale, gene expression profiling methods allow for high throughput analysis of physiological pathways at a fraction of the cost of individual gene expression analysis. Systems, such as the Fluidigm quantitative PCR array described here, can provide powerful assessments of the effects of diet, environment, and management on physiological pathways affecting production parameters. A targeted microfluidic PCR array was designed and validated, for channel catfish (Ictalurus punctatus) representing key pathways involved in appetite, growth, metabolism, and intestinal inflammation for their potential to provide insight into the effects of diet and dietary supplements on these important physiological processes regulating feed efficiency and growth. With few exceptions, PCR primers were designed from Ictaluridae gene sequences published in GenBank. PCR amplicons from primers designed outside of Ictaluridae were sequenced to verify gene identity. All target gene primers were initially validated via conventional real-time qPCR (RT-qPCR). Combined hypothalamus/pituitary, hepatic, stomach, and intestinal tissue were used validate a 48.48 microfluidic PCR array to analyze multitissue gene expression. Use of the Fluidigm array resulted in reliable cycle threshold levels (Ct), efficiencies (E), and quality threshold scores (QS) for all but eight genes examined. Of the potential reference genes included in the panel, alpha-tubulin (TUBA) had a high QS, E, and acceptable Ct. The high throughput application of this technology, relative to conventional RT-qPCR, for assessing dietary effects on these pathways is demonstrated. Development of this targeted multi-tissue microfluidic array paves the way for the rapid evaluation of regulatory pathways in response to alternative feeding strategies, dietary formulations, and supplementation, as well as environmental and management effects for improving channel catfish culture and validates a cost-effective, dynamic, gene expression platform for use with other cultured fishes. (C) 2016 Elsevier B.V. All rights reserved. C1 [Schroeter, Julie C.] Southern Illinois Univ, Dept Anim Sci Food & Nutr, Ctr Fisheries Aquaculture & Aquat Sci, Carbondale, IL 62901 USA. [Small, Brian C.] Univ Idaho, Inst Aquaculture Res, Dept Fish & Wildlife Sci, Hagerman, ID 83332 USA. [Peterson, Brian C.] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. RP Small, BC (reprint author), Univ Idaho, Inst Aquaculture Res, Dept Fish & Wildlife Sci, Hagerman, ID 83332 USA. EM bcsmall@uidaho.edu NR 45 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 213 EP 221 DI 10.1016/j.aquaculture.2016.06.036 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300030 ER PT J AU Anderson, AD Alam, MS Watanabe, WO Carroll, PM Wedegaertner, TC Dowd, MK AF Anderson, A. D. Alam, M. S. Watanabe, W. O. Carroll, P. M. Wedegaertner, T. C. Dowd, M. K. TI Full replacement of menhaden fish meal protein by low-gossypol cottonseed flour protein in the diet of juvenile black sea bass Centropristis striata SO AQUACULTURE LA English DT Article DE Black Sea bass; Gossypol; Cottonseed meal; Growth; Feed utilization; Tissue amino acids and fatty acids; Digestibility ID TROUT ONCORHYNCHUS-MYKISS; FATTY-ACID-COMPOSITION; SOYBEAN-MEAL; RAINBOW-TROUT; GLANDLESS COTTONSEED; CHANNEL CATFISH; SPARUS-AURATA; DIGESTIBILITY COEFFICIENTS; APPARENT DIGESTIBILITY; TRACHINOTUS-CAROLINUS AB Eight isonitrogenous (46% crude protein) and isolipidic (14% crude lipid) diets were formulated for juvenile black sea bass Centropristis striata to replace menhaden fish meal (FM) protein (59.5% CP) by three cottonseed meal (CSM) proteins: a CSM prepared from glandless seed (GCSM, 50.4% CP), a CSM that had been solvent extracted with acidic ethanol to remove the gossypol (SCSM, 53.8% CP), and a CSM prepared from regular (glanded) cottonseed (RCSM, 45% CP). Three diets replaced 50, 75 and 100% of FM protein with GCSM, and three diets replaced 50, 75 and 100% of FM protein with SCSM. One diet replaced 100% FM protein with RCSM protein. A control diet (0% CSM) was formulated with high FM protein and other practical protein sources. L-methionine and L-lysine were supplemented to the diets to equal the control diet. Fifteen fish were stocked in each of twenty-four 75-L tanks, and each test diet was fed to triplicate groups of fish (mean initial weight = 7.7 +/- 0.10 g) for 56 days at 22-24 degrees C, 32-35 salinity, and ambient photoperiod conditions. Fish were fed twice per day to apparent satiation. Final (day 56) mean body weight (BW, 24.16-31.62 g), body weight gain (BWG, 211.2-311.3%), specific growth rate (SGR, 1.70-2.18%/d), and feed intake (FI, 0.37-0.43 g/fish/d) were not significantly different (P > 0.05) among the control, GCSM or SCSM treatments, but were reduced (P < 0.05) for the 100% RCSM treatment (20.09 g, 159.5%, 1.45%/d, and 0.25 g/fish/d, respectively). Lower palatability of the RCSM diet was attributable to the anti-nutrient compound gossypol. No significant differences in survival (84.1-97.8%), feed conversion ratio (FCR, 1.05-1.33), protein efficiency ratio (PER, 1.62-2.08), or whole body protein or lipid composition were observed among the fish fed the low-gossypol diets. Gossypol (25.9 mg/kg) was only detectable in the livers of fish fed the high-gossypol RCSM diet. Fish whole body essential amino acid compositions did not differ significantly among treatments. Whole body n - 3 PUFAs decreased, while n-6 PUFAs increased with increasing CSM protein in the diets. The apparent digestibility coefficient of protein was high (83.1-87.1%) for all treatments. For juvenile black sea bass, 75% FM in the diet can be replaced with low-gossypol CSM protein prepared by solvent-extraction, and 100% of FM can be replaced with low-gossypol CSM protein prepared from glandless seed with no adverse effects on survival, growth or feed utilization. Statement of relevance: Cotton seed flour is an inexpensive agricultural by-product. The results demonstrate successful replacement of fish meal protein with genetically-improved cottonseed flour protein in the diet of black sea bass. These findings may substantially lower aquafeed costs (a key operational cost) for fin fish growout operations to boost profitability and accelerate expansion of commercial production of black sea bass and other fin-fish species. (C) 2016 Elsevier B.V. All rights reserved. C1 [Anderson, A. D.; Alam, M. S.; Watanabe, W. O.; Carroll, P. M.] Univ North Carolina Wilmington, Ctr Marine Sci, Aquaculture Program, 601 S Coll Rd, Wilmington, NC 28403 USA. [Wedegaertner, T. C.] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. [Dowd, M. K.] USDA ARS, New Orleans, LA USA. RP Watanabe, WO (reprint author), Univ North Carolina Wilmington, Ctr Marine Sci, Aquaculture Program, 601 S Coll Rd, Wilmington, NC 28403 USA. EM watanabew@uncw.edu FU Cotton Inc. [13-806] FX This research was supported by Cotton Inc. (Agreement No. 13-806). We thank Dr. Pamela J. Seaton for helpful advice and Macklin Corum, James Hill, Robert Farnell, Jen Gabel, Tyler Gibson, Casey Kelly and Matthew Dawson for technical assistance. NR 67 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD NOV 1 PY 2016 VL 464 BP 618 EP 628 DI 10.1016/j.aquaculture.2016.08.006 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DW0YT UT WOS:000383370300079 ER PT J AU Jones, VP Mills, NJ Brunner, JF Horton, DR Beers, EH Unruh, TR Shearer, PW Goldberger, JR Castagnoli, S Lehrer, N Milickzy, E Steffan, SA Amarasekare, KG Chambers, U Gadino, AN Gallardo, RK Jones, WE AF Jones, Vincent P. Mills, Nicholas J. Brunner, Jay F. Horton, David R. Beers, Elizabeth H. Unruh, Thomas R. Shearer, Peter W. Goldberger, Jessica R. Castagnoli, Steve Lehrer, Nadine Milickzy, Eugene Steffan, Shawn A. Amarasekare, Kaushalya G. Chambers, Ute Gadino, Angela N. Gallardo, R. Karina Jones, Wendy E. TI From planning to execution to the future: An overview of a concerted effort to enhance biological control in apple, pear, and walnut orchards in the western US SO BIOLOGICAL CONTROL LA English DT Editorial Material DE Conservation biological control; Plant volatiles; Pesticide effects on natural enemies; IPM decision-making; Economic analysis; Outreach ID INDUCED PLANT VOLATILES; BENEFICIAL INSECTS; FIELD-EVALUATION; ATTRACTANTS AB We embarked on a large project designed to help enhance biological control in apple, pear and walnut orchards in the western U.S., where management programs were in the midst of a transition from older organo-phosphate insecticides to mating disruption and newer reduced-risk insecticides. A "pesticide replacement therapy" approach resulted in unstable management programs with unpredictable outbreaks of spider mites and aphids. Our project was designed to provide growers and pest managers with information on the effects of newer pesticide chemistries on a suite of representative natural enemies in both the laboratory and field, potential of new monitoring tools using herbivore-induced plant volatiles and floral volatiles, phenology of the key natural enemy species, economic consequences of using an enhanced biological control program, and value of an outreach program to get project outcomes into the hands of decision-makers. We present an overview of both the successes and failures of the project and of new projects that have spun off from this project to further enhance biological control in our systems in the near future. (C) 2016 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Brunner, Jay F.; Beers, Elizabeth H.; Lehrer, Nadine; Steffan, Shawn A.; Chambers, Ute; Gadino, Angela N.; Jones, Wendy E.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Horton, David R.; Unruh, Thomas R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.; Castagnoli, Steve; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Goldberger, Jessica R.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Gallardo, R. Karina] Washington State Univ, Puyallup Res & Extens Ctr, Sch Econ Sci, Puyallup, WA 98371 USA. [Steffan, Shawn A.] Univ Wisconsin, Vegetable Crops Res Unit, USDA ARS, 1575 Linden Dr, Madison, WI 53706 USA. [Lehrer, Nadine] Chatham Univ, Sch Sustainabil, Pittsburgh, PA 15232 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202P,Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu NR 30 TC 4 Z9 4 U1 38 U2 38 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 1 EP 6 DI 10.1016/j.biocontrol.2016.03.013 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200001 ER PT J AU Beers, EH Horton, DR Miliczky, E AF Beers, Elizabeth H. Horton, David R. Miliczky, Eugene TI Pesticides used against Cydia pomonella disrupt biological control of secondary pests of apple SO BIOLOGICAL CONTROL LA English DT Article DE Eriosoma lanigerum; Aphelinus mali; Syrphidae; Neuroptera ID ERIOSOMA-LANIGERUM HAUSMANN; APHID HEMIPTERA APHIDIDAE; OCCIDENTALIS ACARI PHYTOSEIIDAE; REDUCED-RISK INSECTICIDES; INDUCED PLANT VOLATILES; GALENDROMUS-OCCIDENTALIS; ORCHARD PESTICIDES; BENEFICIAL INSECTS; WASHINGTON-STATE; SYNTHETIC PYRETHROIDS AB The effects of codling moth (Cydia pomonella (Linnaeus)) (Lepidoptera: Tortricidae) management programs on secondary pests of apple were examined from 2008 to 2011 in five replicated large-plot trials. The orchards were chosen for a history of Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae) and tetranychid mite outbreaks. Programs covered the first, second, or both generations of C. pomonella, and employed some of the newer insecticides for comparison to older materials. Increased levels of E. lanigerum were consistently associated with the use of spinetoram and novaluron throughout the tests. The mechanism of disruption was not completely clear, but suppression of Forficula auricularia Linnaeus (Dermaptera: Forficulidae) and Aphelinus mali (Haldeman) (Hymenoptera: Aphelinidae) were associated with the use of these materials. Methoxyfenozide, chlorantraniliprole, and azinphos-methyl did not disrupt biological control of tetranychid mites or aphids, and negative effects on natural enemies were infrequent. Lambda-cyhalothrin provided direct control of aphid species, thus reduced levels of natural enemies could also be ascribed to lack of prey. The effect of some of the insecticide applications made during the first generation were evident for many months after the application in terms of pest and natural enemy populations; however, attempts to mitigate disruptive effects by shifting the use of materials to the second generation of C. pomonella were not successful. The effect on biological control of tetranychid mites was less consistent, although some detrimental effects (increased pest or decreased predatory mite levels) were associated with spinetoram, novaluron, and lambda-cyhalothrin. The difficulties in sampling natural enemies and determining their impact made pest outbreaks the most reliable indicator of pesticide-induced disruption.(C) 2016 Elsevier Inc. All rights reserved. C1 [Beers, Elizabeth H.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Miliczky, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Beers, EH (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM ebeers@wsu.edu; david.horton@ars.usda.gov; Gene.Miliczky@ars.usda.gov FU USDA Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission [CP08-805] FX The authors gratefully acknowledge the technical help of Peter Smytheman, Bruce Greenfield, Allie Bock, Alix Whitener, Kaitlin Parsons, Natasza Lorentz, Kylie Martin, Alyssa White, Denise Burnett, Mattie Warner and David Gutierrez in pest and natural enemy sampling. These experiments were funded in part by USDA Specialty Crop Research Initiative Proposal Number 2008-04854 and the Washington Tree Fruit Research Commission (#CP08-805). NR 53 TC 4 Z9 4 U1 24 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 35 EP 43 DI 10.1016/1biocontrol.2016.05.009 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200005 ER PT J AU Beers, EH Mills, NJ Shearer, PW Horton, DR Milickzy, ER Amarasekare, KG Gontijo, LM AF Beers, Elizabeth H. Mills, Nicholas J. Shearer, Peter W. Horton, David R. Milickzy, Eugene R. Amarasekare, Kaushalya G. Gontijo, Lessando M. TI Nontarget effects of orchard pesticides on natural enemies: Lessons from the field and laboratory SO BIOLOGICAL CONTROL LA English DT Article DE Nontarget effects; Biological control; Tetranychidae; Aphididae; Pear psylla ID WORKING GROUP PESTICIDES; BENEFICIAL ORGANISMS; TESTING PROGRAM; BIOLOGICAL-CONTROL; APPLE; INSECTICIDES; TOXICITY; INSECTS; ARTHROPODS; HEMIPTERA AB The nontarget effects of insecticide programs used to control codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), were studied in large-plot field trials in apples, pears, and walnuts in the western United States. We assessed the health of the natural enemy community by sampling the abundance of natural enemies and by monitoring for outbreaks of secondary pests. The insecticides used in the field tests overlapped those tested in laboratory bioassays. Using these parallel lab and field studies, we examined two hypotheses: 1) pesticides found to have negative effects on natural enemy fitness in laboratory bioassays will predict reductions in natural enemy densities in the field, and 2) reductions in natural enemy densities in the field will result in outbreaks of secondary pests. We found only one clear instance, Forficula auricularia (L.) (Dermaptera: Forficulidae), where laboratory results documenting negative effects corresponded to a significant reduction in field studies (apple). This same instance was the only case where a reduction in a natural enemy population was associated with a significantly increased density of a secondary pest, Eriosoma lanigerum (Hausmann) (Hemiptera: Aphididae). There were several instances where secondary pest outbreaks were associated with unchanged or even increased natural enemy densities. The limited number of field trials, variability in field trial conditions among years and sites, duration of the negative effect relative to sampling interval, sampling efficiency, plot size/inter-plot movement, and compensation by other natural enemies attracted to a large host/prey resource may all have contributed to the poor predictive success. Overall, the laboratory bioassays predicted a far greater negative impact than was found in the field trials. (C) 2016 Elsevier Inc. All rights reserved. C1 [Beers, Elizabeth H.; Gontijo, Lessando M.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Horton, David R.; Milickzy, Eugene R.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.] 1523 Lincoln St Unit 200, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202 P Farrell Westbrook Hall, Nashville, TN 37209 USA. [Gontijo, Lessando M.] Univ Fed Vicosa, Campus Florestal, BR-35690000 Florestal, MG, Brazil. RP Beers, EH (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM ebeers@wsu.edu; nmills@berkeley.edu; shearpw@gmail.com; david.horton@ars.usda.gov; kamarase@tnstate.edu; lessandomg@gmail.com FU USDA Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission FX The authors gratefully acknowledge the technical help of Peter Smytheman and Bruce Greenfield, as well as undergraduates Allie Bock, Alix Crilly, Kaitlin Parsons, Natasza Lorentz, Kylie Martin, Alyssa White, Denise Burnett, Mattie Warner and David Gutierrez in pest and natural enemy sampling. We thank Chris Sater for assistance in proofreading and graphics preparation. These experiments were funded in part by USDA Specialty Crop Research Initiative Proposal Number 2008-04854 and the Washington Tree Fruit Research Commission. NR 41 TC 8 Z9 8 U1 15 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 44 EP 52 DI 10.1016/j.biocontrol.2016.04.010 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200006 ER PT J AU Jones, VP Horton, DR Mills, NJ Unruh, TR Baker, CC Melton, TD Milickzy, E Steffan, SA Shearer, PW Amarasekare, KG AF Jones, Vincent P. Horton, David R. Mills, Nicholas J. Unruh, Thomas R. Baker, Callie C. Melton, Tawnee D. Milickzy, Eugene Steffan, Shawn A. Shearer, Peter W. Amarasekare, Kaushalya G. TI Evaluating plant volatiles for monitoring natural enemies in apple, pear and walnut orchards SO BIOLOGICAL CONTROL LA English DT Article DE Herbivore induced plant volatiles; Floral volatiles; Natural enemy monitoring ID IPM DECISION-MAKING; BENEFICIAL INSECTS; METHYL SALICYLATE; GREEN LACEWINGS; SAMPLING PLANS; CARNIVOROUS ARTHROPODS; BIOLOGICAL-CONTROL; FIELD-EVALUATION; CHRYSOPERLA; CHRYSOPIDAE AB The ability to estimate natural enemy abundance is crucial to the integration of biological control into IPM programs. Traditional sampling approaches for natural enemies are few and most are inefficient, but recent studies suggest attraction of natural enemies to plant volatiles may be a useful proxy for direct sampling. We evaluated various combinations of herbivore-induced plant volatiles and floral volatiles as monitoring tools for natural enemies found in apple, pear, and walnut orchards in California, Oregon, and Washington. In 2010 we used a full factorial experimental designs to evaluate lures for all combinations of acetic acid (AA), acetophenone (AP), phenylacetaldehyde (PAA) and 2-phenylethanol (PE). Of nine natural enemy taxa analyzed, we found syrphid flies responded strongly to PE, but combining AA with PE attenuated trap catch and combining PAA to PE eliminated the activity of PE. Chlysoperla spp. (Chrysopidae) responded strongly to most of the individual compounds and the various interactions between the components allowed multiple ways to achieve roughly the same trap catch. All of the hymenopteran taxa collected responded strongly to PAA, and PAA containing lures were nearly always a component of the top eight lures. A smaller factorial experiment testing all possible combinations of AA, PAA and methyl salicylate (MS) showed that single component AA or MS lures were generally not significantly different than the controls for all taxa tested, but for the hymenopteran taxa, traps baited with MS+PAA performed the best or were not significantly different than the best performing lure. A 2011 trial was conducted to test the influence of the addition of AA and/or MS on previously tested lures. Combining AA or MS with other lures, improved the capture of Chrysoperla spp.; Scaeva pyrastri (L.) (Syrphidae) capture was enhanced when MS was used with PE; and PE was attractive to the three syrphid flies, anysoperla spp., and the parasitoid Aphelinus mall (Haldeman) (Aphelinidae). The differential responses to various blends exhibited among taxa show that combinations of plant volatiles can be chosen to increase specificity of attraction to a few taxa or increase the number of species attracted. This flexibility should add to the general value and breadth of use of plant volatile monitoring lures. (C) 2015 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.; Steffan, Shawn A.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Unruh, Thomas R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu FU USDA-Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board FX This work was supported in part by USDA-Specialty Crop Research Initiative grant 2008-04854, and grants from the Washington Tree Fruit Research Commission (to VPJ), California Walnut Board (to NJM). We thank Merilee Bayer, Deb Broers, Preston Brown, Lisa Fernandez, Aviva Goldmann, Kodi Jaspers, Laura Jones, Kevi Mace-Hill, Stacey McDonald, Francisco De La Rosa, Teah Smith, for technical support. NR 50 TC 7 Z9 7 U1 31 U2 31 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 53 EP 65 DI 10.1016/j.biocontrol.2015.03.009 PG 13 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200007 ER PT J AU Mills, NJ Jones, VP Baker, CC Melton, TD Steffan, SA Unruh, TR Horton, DR Shearer, PW Amarasekare, KG Milickzy, E AF Mills, Nicholas J. Jones, Vincent P. Baker, Callie C. Melton, Tawnee D. Steffan, Shawn A. Unruh, Thomas R. Horton, David R. Shearer, Peter W. Amarasekare, Kaushalya G. Milickzy, Eugene TI Using plant volatile traps to estimate the diversity of natural enemy communities in orchard ecosystems SO BIOLOGICAL CONTROL LA English DT Article DE Apple; Evenness; Parasitoid; Pear; Predator; Richness; Walnut ID CONSERVATION BIOLOGICAL-CONTROL; ZEALAND APPLE ORCHARDS; PEST-MANAGEMENT; CARABIDS COLEOPTERA; BIODIVERSITY; EVENNESS; ARTHROPODS; PREDATORS; SERVICES; IMPACT AB In this study we used sticky traps baited with plant volatile lures to monitor the biodiversity of natural enemies in orchard ecosystems in the western U.S. We compared the diversity of predator genera from season total trap catches in 37 different orchards (apple, cherry, pear and walnut) over a two-year period (2010-2011) using standardized Hill number biodiversity indices and community similarity profiles. For a subset of 23 of these orchards we were also able to monitor the change in biodiversity of predator genera over the full growing season in the different orchard crops. A total of 37,854 individuals from 31 different genera of foliage-active generalist predators were collected from all orchards combined. Mean sample coverage was high (0.98) and richness, diversity and evenness differed between crops in 2010, but not in 2011. There was more than 90% similarity in the richness of predator genera among crops and among orchards within crops, but a greater level of differentiation was observed among orchards when variation in their relative abundance and dominance in the communities was taken into account. There was a consistent rise in predator generic richness and diversity through the season in both years for apple, cherry and pear orchards, but in walnut orchards, a steep rise from March to May was followed by a decline through the rest of the season. In an additional component of the study, the species level similarity of predator and parasitoid communities was analyzed for total season trap catch data from six walnut orchards. The rarefied species richness of parasitoids was much greater than that for predators, although the diversity, evenness and dominance of the parasitoid species varied considerably among orchards. The results from this study highlight the fact that natural enemy communities in orchard ecosystems can be effectively monitored using plant volatile traps, and that these communities are surprisingly diverse despite frequent disturbance from pest management intervention. (C) 2016 Elsevier Inc. All rights reserved. C1 [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.; Steffan, Shawn A.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Unruh, Thomas R.; Horton, David R.; Milickzy, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Shearer, Peter W.; Amarasekare, Kaushalya G.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202P,Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Mills, NJ (reprint author), Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. EM nmills@berkeley.edu FU USDA-SCRI grant [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board; pear growers in Hood River (Oregon) FX This research was supported by USDA-SCRI grant 2008-04854 and matching funds from the Washington Tree Fruit Research Commission, California Walnut Board and pear growers in Hood River (Oregon). The research personnel and technical support of the laboratories from the University of California at Berkeley, Oregon State University, Washington State University and USDA-ARS Yakima are gratefully acknowledged. NR 70 TC 2 Z9 2 U1 20 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 66 EP 76 DI 10.1016/j.biocontrol.2016.05.001 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200008 ER PT J AU Jones, VP Horton, DR Mills, NJ Unruh, TR Miliczky, E Shearer, PW Amarasekare, KG Baker, CC Melton, TD AF Jones, Vincent P. Horton, David R. Mills, Nicholas J. Unruh, Thomas R. Miliczky, Eugene Shearer, Peter W. Amarasekare, Kaushalya G. Baker, Callie C. Melton, Tawnee D. TI Using plant volatile traps to develop phenology models for natural enemies: An example using Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) SO BIOLOGICAL CONTROL LA English DT Article DE Chrysopa nigricornis; Phenology model; Herbivore-induced plant volatiles; Apple; Walnut; Pear; Sweet cherry ID CODLING MOTH LEPIDOPTERA; BENEFICIAL INSECTS; METHYL SALICYLATE; CENTRAL WASHINGTON; FIELD-EVALUATION; PEST-MANAGEMENT; ATTRACTANTS; TEMPERATURE; PREDATORS; LACEWINGS AB A model predicting phenology of adult Chrysopa nigricornis (Burmeister) (Neuroptera: Chrysopidae) in orchards was developed from field (trapping) data supplemented with development data collected under laboratory conditions. Lower and upper thresholds of 10.1 degrees C and 29.9 degrees C, respectively, were estimated from published and unpublished laboratory observations, and were used to develop a phenology model. Season-long field data were collected using white delta traps that had been baited with squalene, a volatile shown previously to be highly attractive to C. nigricornis. The model was developed from data collected in three Washington apple orchards, and was validated using independent data sets collected from apple, sweet cherry, pear, and walnut orchards over a 2-4 year period across a much wider geographic region. We found that the mean absolute deviation across all crops and years was 39.7 +/- 1.2 day-degrees (DD), or 4.4 +/- 0.14 days. Populations of C nigricornis from walnut orchards in California emerged 105 DD later than those in Oregon and Washington, thus requiring correction of average time of first trap catch in California to synchronize models. The ability to use a single model across multiple crops, different prey species and abundances, and different pesticide regimes demonstrates that phenology models for generalist predators may have broader application to IPM programs in other cropping systems. (C) 2015 Elsevier Inc. All rights reserved. C1 [Jones, Vincent P.; Baker, Callie C.; Melton, Tawnee D.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. [Horton, David R.; Unruh, Thomas R.; Miliczky, Eugene] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Mills, Nicholas J.] Univ Calif Berkeley, Dept Environm Sci & Policy Management, Berkeley, CA 94720 USA. [Shearer, Peter W.] Oregon State Univ, Mid Columbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. [Amarasekare, Kaushalya G.] Tennessee State Univ, Dept Agr & Environm Sci, Room 202 P Farrell Westbrook Hall, Nashville, TN 37209 USA. RP Jones, VP (reprint author), Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. EM vpjones@wsu.edu; kamarase@tnstate.edu FU USDA-Specialty Crop Research Initiative [2008-04854]; Washington Tree Fruit Research Commission; California Walnut Board FX This work was supported in part by USDA-Specialty Crop Research Initiative Grant 2008-04854, and Grants from the Washington Tree Fruit Research Commission (to VPJ), California Walnut Board (to NJM). We thank Merilee Bayer, Deb Broers, Preston Brown, Lisa Fernandez, Aviva Goldmann, Kodi Jaspers, Laura Jones, Kevi Mace-Hill, Stacey McDonald, Francisco De La Rosa, Teah Smith, and Amanda Borel for technical support. NR 47 TC 5 Z9 5 U1 9 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 77 EP 84 DI 10.1016/j.biocontrol.2014.12.012 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200009 ER PT J AU Unruh, TR Miliczky, ER Horton, DR Thomsen-Archer, K Rehfield-Ray, L Jones, VP AF Unruh, Thomas R. Miliczky, Eugene R. Horton, David R. Thomsen-Archer, Kelly Rehfield-Ray, Linda Jones, Vincent P. TI Gut content analysis of arthropod predators of codling moth in Washington apple orchards SO BIOLOGICAL CONTROL LA English DT Article DE Cydia pomonella; Predatory arthropods; Polymerase chain reaction; Conservation biological control ID POLYMERASE-CHAIN-REACTION; DETECTABILITY HALF-LIFE; COLORADO POTATO BEETLE; GENERALIST PREDATORS; BIOLOGICAL-CONTROL; CYDIA-POMONELLA; DIRECT-PCR; FORFICULA-AURICULARIA; LACANOBIA-SUBJUNCTA; MOLECULAR ANALYSIS AB Codling moth, Cydia pomonella (L.), is the key pest of pome fruits in many temperate areas of North America, Eurasia, South Africa, South America and Australia. Many predatory arthropods species are found in organic apple orchards of central Washington; here we use PCR-based gut content analysis of arthropod predators to identify predators that attack codling moth. Predators were sampled from tree canopies, tree trunks and from the understory and were homogenized in a lysis buffer to provide a template for Direct PCR. PCR showed 8.9% of 2591 predators had preyed on C. pomonella. Spiders, including 25 genera from 15 families, two carabid beetle species (Pterostichus melanarius (Illiger) and Harpalus penn-sylvanicus DeGeer) and the European earwig (Forficula auricularia [L.]) represented 87% of predator specimens analyzed and were 8.2%, 8.3% and 14.7% positive for C pomonella. PCR products from 38% of predators that appeared positive for C. pomonella COI were sequenced; all showed 99% or more similarity to C. pomonella COI sequences in GenBank. Digestion rates of adult earwigs fed on mature codling moth larvae showed a detection half-life of 3.7 days; half-life from the fecal pellets from the same earwigs was 3.6 days. When fed mature codling moth larvae, the carabid P. melanarius showed a digestion half-life of 3.14 days. Identification of the key predators of C. pomonella can guide the use of selective insecticides and the conservation of these natural enemies, enhancing biological control and supporting stable IPM programs in pome fruit orchards in the western USA. Published by Elsevier Inc. C1 [Unruh, Thomas R.; Miliczky, Eugene R.; Horton, David R.; Thomsen-Archer, Kelly; Rehfield-Ray, Linda] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Jones, Vincent P.] Washington State Univ, Dept Entomol, Tree Fruit Res & Extens Ctr, 1100 N Western Ave, Wenatchee, WA 98801 USA. RP Unruh, TR (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM thomas.unruh@ars.usda.gov FU Washington Tree Fruit Research Commission; USDA Specialty Crop Research Initiative FX This manuscript was greatly improved by editorial and analytical suggestions provided by Nicholas Mills of the University of California at Berkeley. Editorial suggestions by James Hagler, USDA, ARS, Maricopa AZ and Marshal W.Johnson, University of California, Riverside, emeritus, and three anonymous reviewers also greatly helped improve the manuscript. Funding by the Washington Tree Fruit Research Commission and the USDA Specialty Crop Research Initiative made the study possible. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 84 TC 2 Z9 2 U1 29 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD NOV PY 2016 VL 102 BP 85 EP 92 DI 10.1016/j.biocontrol.2016.05.014 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DW8XG UT WOS:000383938200010 ER PT J AU Marwarha, G Claycombe, K Schommer, J Collins, D Ghribi, O AF Marwarha, Gurdeep Claycombe, Kate Schommer, Jared Collins, David Ghribi, Othman TI Palmitate-induced Endoplasmic Reticulum stress and subsequent C/EBP alpha Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain SO CELLULAR SIGNALLING LA English DT Article DE Endoplasmic Reticulum stress; Leptin; Insulin-like growth factor 1; Palmitic acid; CCAAT Enhancer Binding Protein alpha; C/EBP Homologous Protein ID ADULT HIPPOCAMPAL NEUROGENESIS; DOMINANT-NEGATIVE INHIBITOR; DIETARY FATTY-ACIDS; ALZHEIMERS-DISEASE; FACTOR-I; HUNTINGTONS-DISEASE; TAU PHOSPHORYLATION; MITOCHONDRIAL-FUNCTION; TRANSCRIPTION FACTORS; PARKINSONS-DISEASE AB The peptide hormones Insulin-like growth factor-1 (IGF1) and leptin mediate a myriad of biological effects - both in the peripheral and central nervous systems. The transcription of these two hormones is regulated by the transcription factor C/EBP alpha, which in turn is negatively regulated by the transcription factor C/EBP Homologous Protein (CHOP), a specific marker of endoplasmic reticulum (ER) stress. In the peripheral system, disturbances in leptin and IGF-1 levels are implicated in a variety of metabolic diseases including obesity, diabetes, atherosclerosis and cardiovascular diseases. Current research suggests a positive correlation between consumption of diets rich in saturated free fatty acids (sFFA) and metabolic diseases. Induction of ER stress and subsequent dysregulation in the expression levels of leptin and IGF-1 have been shown to mediate sFFA-induced metabolic diseases in the peripheral system. Palmitic acid (palmitate), the most commonly consumed sFFA, has been shown to be up taken by the brain, where it may promote neurodegeneration. However, the extent to which palmitate induces ER stress in the brain and attenuates leptin and IGF1 expression has not been determined. We fed C57BL/6J mice a palmitate-enriched diet and determined effects on the expression levels of leptin and IGF1 in the hippo campus and cortex. We further determined the extent to which ER stress and subsequent CHOP activation mediate the palmitate effects on the transcription of leptin and IGF1. We demonstrate that palmitate induces ER stress and decreases leptin and IGF1 expression by inducing the expression of CHOP. The molecular chaperone 4-phenylbutyric acid (4-PBA), an inhibitor of ER stress, precludes the palmitate-evoked down-regulation of leptin and IGF1 expression. Furthermore, the activation of CHOP in response to ER stress is pivotal in the attenuation of leptin and IGF1 expression as knocking-down CHOP in mice or in SH-SY5Y and Neuro-2a (N2a) cells rescues the palmitate-induced mitigation in leptin and IGF1 expression. Our study implicates for the first time ER stress-induced CHOP activation in the brain as a mechanistic link in the palmitate-induced negative regulation of leptin and IGF1, two neurotrophic cytokines that play an indispensable role in the mammalian brain. (C) 2016 Elsevier Inc. All rights reserved. C1 [Marwarha, Gurdeep; Schommer, Jared; Collins, David; Ghribi, Othman] Univ North Dakota, Sch Med & Hlth Sci, Dept Biomed Sci, Grand Forks, ND 58203 USA. [Claycombe, Kate] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. RP Ghribi, O (reprint author), Univ North Dakota, Dept Biomed Sci, Sch Med & Hlth Sci, 501 North Columbia Rd, Grand Forks, ND 58202 USA. EM othman.ghribi@med.und.edu OI MARWARHA, GURDEEP/0000-0001-5556-0003 FU National Institutes of Health [R01AG0145264] FX This work was supported by National Institutes of Health, Grant # R01AG0145264, to Dr. Othman Ghribi. NR 70 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0898-6568 EI 1873-3913 J9 CELL SIGNAL JI Cell. Signal. PD NOV PY 2016 VL 28 IS 11 BP 1789 EP 1805 DI 10.1016/j.cellsig.2016.08.012 PG 17 WC Cell Biology SC Cell Biology GA DW8YU UT WOS:000383942200018 PM 27555288 ER PT J AU Kim, S Williams, A Kiniry, JR Hawkes, CV AF Kim, Sumin Williams, Amber Kiniry, James R. Hawkes, Christine V. TI Simulating diverse native C-4 perennial grasses with varying rainfall SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Forage; Rainfall; ALMANAC; Plant growth; Perennial grass ID PLANT-COMMUNITIES; NORTH-AMERICAN; WATER-USE; PRECIPITATION; PRODUCTIVITY; SWITCHGRASS; EFFICIENCY; RESPONSES; LIVESTOCK; BIOMASS AB Rainfall is recognized as a major factor affecting the rate of plant growth development. The impact of changes in amount and variability of rainfall on growth and production of different forage grasses needs to be quantified to determine how climate change can impact rangelands. Comparative studies to evaluate the growth of several perennial forage species at different rainfall rates will provide useful information by identifying forage management strategies under various rainfall scenarios. In this study, the combination of rainfall changes and soil types on the plant growth of 10 perennial forage species was investigated with both the experimental methods, using rainout shelters, and with the numerical methods using the plant growth simulation model, ALMANAC. Overall, most species significantly increased basal diameter and height as rainfall increased. Like measured volume, simulated yields for all species generally increased as rainfall increased. But, large volume and yield increases were only observed between 350 and 850 mm/yr. Simulating all species growing together competing agrees relatively well with observed plant volumes at low rainfall treatment, while simulating all species growing separately was slightly biased towards overestimation on low rainfall effect. Both simulations agree relatively well with observed plant volume at high rainfall treatment. Published by Elsevier Ltd. This is an open access article under the CC BY license. C1 [Kim, Sumin] Oak Ridge Inst Sci & Educ, Oak Ridge, TN 37831 USA. [Williams, Amber; Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Hawkes, Christine V.] Univ Texas Austin, Sect Integrat Biol, Austin, TX 78712 USA. RP Kiniry, JR (reprint author), USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA.; Hawkes, CV (reprint author), Univ Texas Austin, Sect Integrat Biol, Austin, TX 78712 USA. EM Jim.Kiniry@ARS.USDA.GOV; chawkes@austin.utexas.edu OI Williams, Amber/0000-0001-6898-8543 FU Department of Energy (DOE) [08-SC-NICCR-1071]; Interagency Reimbursable Agreement [60-3098-5-006]; U.S. Department of Energy; U.S. Department of Agriculture, Agricultural Research Service Agreement [60-3098-5-002] FX We are grateful to Nick Johnson, Elise Worchel, Hannah Giauque, Lauren Taranow, Gabe Miller, and other members of the Hawkes lab who assisted with data collection. We also thank Stephanie Silvia, Civil Engineering department at University of Texas at San Antonio, who did some of the preliminary analyses. Funding for the shelter experiment was provided by the Department of Energy (DOE) #08-SC-NICCR-1071 to CVH. This work was funded by Interagency Reimbursable Agreement #60-3098-5-006. This work was also supported in part by an appointment to Agricultural Research Service administrated by Oak Ridge Institute for Science and Education through interagency agreement between U.S. Department of Energy and U.S. Department of Agriculture, Agricultural Research Service Agreement #60-3098-5-002. NR 50 TC 0 Z9 0 U1 6 U2 6 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD NOV PY 2016 VL 134 BP 97 EP 103 DI 10.1016/j.jaridenv.2016.07.004 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DW8WH UT WOS:000383935700013 ER PT J AU Edwin, ES Vasantha-Srinivasan, P Senthil-Nathan, S Thanigaivel, A Ponsankar, A Pradeepa, V Selin-Rani, S Kalaivani, K Hunter, WB Abdel-Megeed, A Duraipandiyan, V Al-Dhabi, NA AF Edwin, Edward-Sam Vasantha-Srinivasan, Prabhakaran Senthil-Nathan, Sengottayan Thanigaivel, Annamalai Ponsankar, Athirstam Pradeepa, Venkatraman Selin-Rani, Selvaraj Kalaivani, Kandaswamy Hunter, Wayne B. Abdel-Megeed, Ahmed Duraipandiyan, Veeramuthu Al-Dhabi, Naif Abdullah TI Anti-dengue efficacy of bioactive andrographolide from Andrographis paniculata (Lamiales: Acanthaceae) against the primary dengue vector Aedes aegypti (Diptera: Culicidae) SO ACTA TROPICA LA English DT Article DE Dengue; Bioactive; RP-HPLC; Repellent; Mortality; Enzyme; Histology ID CULEX-QUINQUEFASCIATUS SAY.; ESSENTIAL OILS; ENZYME-ACTIVITIES; IN-VITRO; MOSQUITO; EXTRACT; PLANTS; LARVAE; INSECTICIDE; REPELLENT AB The current study investigated the toxic effect of the leaf extract compound andrographolide from Andrographis paniculata (Burm.f) against the dengue vector Ae. aegypti. GC-MS analysis revealed that andrographolide was recognized as the major chemical constituent with the prominent peak area compared with other compounds. All isolated toxic compounds were purified and confirmed through RP-HPLC against chemical standards. The larvicidal assays established at 25 ppm of bioactive compound against the treated instars of Ae. Aegypti showed prominent mortality compared to other treated concentrations. The percent mortality of larvae was directly proportional to concentration. The lethal concentration (LC50) was observed at 12 ppm treatment concentration. The bioactive andrographolide considerably reduced the detoxifying enzyme regulations of alpha- and beta- carboxylesterases. In contrast, the levels of GST and CYP450 significantly increase in a dose dependent manner. The andrographolide also showed strong oviposition deterrence effects at the sub-lethal dose of 12 ppm. Similarly, the mean number of eggs were also significantly reduced in a dose dependent manner. At the concentration of 12 ppm the effective percentage of repellency was greater than 90% with a protection time of 15-210 min, compared with control. The histopathology study displayed that larvae treated with bioactive andrographolide had cytopathic effects in the midgut epithelium compared with the control. The present study established that bioactive andrographolide served as a potential useful for dengue vector management. (C) 2016 Elsevier B.V. All rights reserved. C1 [Edwin, Edward-Sam; Vasantha-Srinivasan, Prabhakaran; Senthil-Nathan, Sengottayan; Thanigaivel, Annamalai; Ponsankar, Athirstam; Pradeepa, Venkatraman; Selin-Rani, Selvaraj] Manonmaniam Sundaranar Univ Alwarkurichi, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. [Kalaivani, Kandaswamy] Sri Parasakthi Coll Women, Dept Zool, Post Grad & Res, Tirunelveli 627802, Tamil Nadu, India. [Hunter, Wayne B.] USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Abdel-Megeed, Ahmed] Alexandria Univ, Saba Basha, Fac Agr, Dept Plant Protect, POB 21531, Alexandria 21526, Egypt. [Edwin, Edward-Sam; Duraipandiyan, Veeramuthu; Al-Dhabi, Naif Abdullah] King Saud Univ, Coll Sci, Addiriyah Chair Environm Studies, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. RP Senthil-Nathan, S (reprint author), Manonmaniam Sundaranar Univ Alwarkurichi, Sri Paramakalyani Ctr Excellence Environm Sci, Div Biopesticides & Environm Toxicol, Tirunelveli 627412, Tamil Nadu, India. EM senthil@msuniv.ac.in RI Veeramuthu, Duraipandiyan/D-9924-2012; OI Veeramuthu, Duraipandiyan/0000-0002-2107-3503; Abdel-Megeed, Ahmed/0000-0002-9777-4808 FU King Saud University; Vice Deanship of Research Chair FX The project was full financially supported by King Saud University, through Vice Deanship of Research Chair. NR 56 TC 0 Z9 0 U1 16 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD NOV PY 2016 VL 163 BP 167 EP 178 DI 10.1016/j.actatropica.2016.07.00 PG 12 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA DW3FO UT WOS:000383527700025 PM 27443607 ER PT J AU Garcia-Fuentes, PA Kennedy, PL Ferreira, GFC AF Garcia-Fuentes, Pablo A. Kennedy, P. Lynn Ferreira, Gustavo F. C. TI US foreign direct investment in Latin America and the Caribbean: a case of remittances and market size SO APPLIED ECONOMICS LA English DT Article DE Foreign direct investment; remittances; market size; United States; Latin America and the Caribbean ID EXCHANGE-RATES; ECONOMETRIC-ANALYSIS; DEVELOPING-COUNTRIES; DETERMINANTS; TRADE; GROWTH; FLOWS; TIME AB This article investigates the effect of remittances on U.S. foreign direct investment (FDI) flows to Latin America and the Caribbean (LAC). It covers 26 countries for the period 1983-2010. The results show a positive and significant impact of remittances on U.S. FDI flows. However, this effect depends upon the level of gross domestic product (GDP) per capita of the host country. On average, the results show that increasing remittances by one standard deviation increases U.S. FDI flows by 0.44 percent a year. Also, host country demand positively affects U.S. FDI flows, which supports the market size hypothesis. C1 [Garcia-Fuentes, Pablo A.] Midwestern State Univ, Dillard Coll Business Adm 215, Dept Econ, Wichita Falls, TX 76308 USA. [Kennedy, P. Lynn] Louisiana State Univ, Dept 0gr Econ & Agribusiness, Baton Rouge, LA 70803 USA. [Ferreira, Gustavo F. C.] USDA, Econ Res Serv, Crops Branch, Washington, DC 20002 USA. RP Ferreira, GFC (reprint author), USDA, Econ Res Serv, Crops Branch, Washington, DC 20002 USA. EM gustavo.ferreira@ers.usda.gov NR 49 TC 0 Z9 0 U1 15 U2 15 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0003-6846 EI 1466-4283 J9 APPL ECON JI Appl. Econ. PD NOV PY 2016 VL 48 IS 51 BP 5008 EP 5021 DI 10.1080/00036846.2016.1170931 PG 14 WC Economics SC Business & Economics GA DW2AB UT WOS:000383444200007 ER PT J AU Itkin, D Goldfus, H Monger, HC AF Itkin, Danny Goldfus, Haim Monger, H. Curtis TI Human induced calcretisation in anthropogenic soils and sediments: Field observations and micromorphology in a Mediterranean climatic zone, Israel SO CATENA LA English DT Article DE Calcrete; Pedogenic carbonate; Calcrete cement; Geoarchaeology; Soil micromorphology ID CALCIUM-OXALATE; CALCRETE FORMATION; CARBONATE; QUATERNARY; PRECIPITATION; ECOSYSTEM; PLANTS; BIOMINERALIZATION; TRANSFORMATIONS; MINERALIZATION AB For over a century, the study of pedogenic calcrete end its formation focuses on natural processes, disregarding the possible role of humans in its formation. Here we present field and micromorphological evidence from archaeological and modern sites in the eastern Mediterranean region (Israel) that indicate that some specific calcretes are human induced. We demonstrate that anthropogenic affected calcrete differs from 'natural' (non-human induced) calcrete. We show that the occurrence of the human induced calcrete is spatially limited and is associated with both macro- and micro-scale remnants, and occasionally high content of organic material. We propose an operative definition for anthropogenic calcretisation as the interference of humans with a component(s) that influence the formation of calcrete. We assume that if present-day calcretised environments will retain their calcretisation prone conditions for the next 5-10 millennia, a distinct calcretic morphology could become a significant terrestrial formation. This discovery can be particularly useful for evaluating past human impact on the environment, adding a new archaeological set of research tools that should further be developed. (C) 2016 Elsevier B.V. All rights reserved. C1 [Itkin, Danny; Goldfus, Haim] Ben Gurion Univ Negev, Dept Bible Archaeol & Ancient Near East, Beer Sheva, Israel. [Monger, H. Curtis] USDA, Natl Soil Survey Ctr, Lincoln, NE USA. RP Itkin, D (reprint author), Ben Gurion Univ Negev, Dept Bible Archaeol & Ancient Near East, Beer Sheva, Israel. EM danitkin@gmail.com; hgoldfus@bgu.ac.il; curtis.monger@lin.usda.gov OI Itkin, Danny/0000-0002-1732-6950 NR 112 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 48 EP 61 DI 10.1016/j.catena.2016.06.025 PG 14 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400005 ER PT J AU Brevik, EC Homburg, JA Miller, BA Fenton, TE Doolittle, JA Indorante, SJ AF Brevik, Eric C. Homburg, Jeffrey A. Miller, Bradley A. Fenton, Thomas E. Doolittle, James A. Indorante, Samuel J. TI Selected highlights in American soil science history from the 1980s to the mid-2010s SO CATENA LA English DT Article DE Soil science history; Pedoarchaeology; Digital soil mapping; Soil and the environment; Geophysical instrumentation; Remote sensing ID PREHISTORIC AGRICULTURAL TERRACES; GROUND-PENETRATING RADAR; UNITED-STATES; CARBON SEQUESTRATION; ORGANIC-CARBON; ELECTRICAL-CONDUCTIVITY; NEW-MEXICO; SURVEY INFORMATION; CLIMATE-CHANGE; SUBAQUEOUS SOILS AB The beginning of the 20th century through the 1970s were good times for soil science in the USA, with relatively strong funding and overall growth in the profession. However, the soil science discipline in the USAhit hard times in the 1980s and 1990s. Federal funding for soil survey work began to decline as did student numbers in university programs and membership in the Soil Science Society of America (SSSA). Despite this, there were still many positive advances within soil science in theUSA during these two decades. There was an increased use of geophysical instrumentation, remote sensing, geographic information systems (GIS), and global positioning systems (GPS), and research began in digital soil mapping, all of which lead to better understanding of the spatial distribution and variability of soils. Many NRCS soil products were put online, making them widely available to the general public, the use of soil knowledge was expanded into new areas such as archaeology and environmental work, and historic connections to geology were re-established. While expansion into new areas required soil science to evolve as a field, separating the discipline to an extent from its agricultural roots, it also helped reinvigorate the discipline. As we move through the early parts of the 21st century, student numbers are increasing in university soil science programs and membership in SSSA is at an all-time high. Digital soil mapping is being incorporated into the National Cooperative Soil Survey, and,the impact of humans on the soil system is being fully recognized. The importance of soils is being recognized by events such as the United Nations declaration of 2015 as the "International Year of Soils". The expansion of soils into new areas and widening recognition of the importance of soils gives the field hope for a bright future in the USA. (C) 2016 Elsevier B.V. All rights reserved. C1 [Brevik, Eric C.] Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. [Homburg, Jeffrey A.] Stat Res Inc, Tucson, AZ USA. [Homburg, Jeffrey A.] Univ Arizona, Sch Anthropol, Tucson, AZ USA. [Miller, Bradley A.; Fenton, Thomas E.] Iowa State Univ, Dept Agron, Ames, IA USA. [Miller, Bradley A.] Leibniz Ctr Agr Landscape Res ZALF eV, Inst Soil Landscape Res, Eberswalder Str 84, D-15374 Muncheberg, Germany. [Doolittle, James A.] USDA NRCS NSSC, 11 Campus Blvd,Suite 200, Newtown Sq, PA USA. [Indorante, Samuel J.] USDA NRCS, Du Quoin, IL USA. RP Brevik, EC (reprint author), Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. EM Eric.Brevik@dickinsonstate.edu; jhomburg@sricrm.com; millerba@iastate.edu; tefenton@iastate.edu; sjindorante@siu.edu FU National Science Foundation [IIA-1355466] FX The authors wish to acknowledge the influence of Dan H. Yaalon in growing the study of soil science history. The early seeds for this project were planted in part during conversations between E. Brevik and D. Yaalon. We thank Pam Thomas (USDA-NRCS) for supplying Federal soil survey budget information and Jon Sandor for review of an early draft and helpful comments that improved the paper. We also thank Johan Bouma and an anonymous reviewer for their helpful comments on the manuscript. E.C. Brevik was partially supported by the National Science Foundation under Grant Number IIA-1355466 during this project. NR 284 TC 0 Z9 0 U1 38 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 128 EP 146 DI 10.1016/j.catena.2016.06.021 PG 19 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400012 ER PT J AU Brevik, EC Richter, DD Verrecchia, EP Ryan, J Poch, RM Crouvi, O Sauer, D Waroszewski, J Solleiro-Rebolledo, E Monger, C Ottner, F Targulian, V AF Brevik, Eric C. Richter, Daniel deB Verrecchia, Eric P. Ryan, John Poch, Rosa M. Crouvi, Onn Sauer, Daniela Waroszewski, Jaroslaw Solleiro-Rebolledo, Elizabeth Monger, Curtis Ottner, Franz Targulian, Victor TI The influence of Dan H. Yaalon: His impact on people SO CATENA LA English DT Article DE Pedology; Paleosols; Soil science history; Aeolian processes; Scientific contributions; Scientific influence ID REG SOILS; DESERT; LOESS; MODEL; DUST AB There are many ways to measure the accomplishments of a scientist, such as papers and books published, citations, and prestige of journals published in, but we rarely gather information that allows us to evaluate the variety of influences that individual scientists have on the careers of others. Dan H. Yaalon was one of the most accomplished pedologists of the 20th and early 21st centuries. In this paper we gather personal testimonials of the impact Yaalon had on scientists working in the areas of interest to Yaalon. This allows us to qualitatively evaluate the impact of an influential scientist on his field through the ways that he influenced the career choices of others. We found that this influence occurred through 1) personal, face-to-face contact, 2) written correspondence between Yaalon and other scientists, both through letters and through email, and 3) Yaalon's written record itself, without any direct, personal interaction between Yaalon and the other scientists. This influence took place over multiple generations of scientists, ranging from individuals who are currently retired to those who are just now in the very early stages of their scientific careers. The Yaalon example makes clear how successful scientists make significant contributions to their fields of interest well beyond those indicated by commonly used measures. (C) 2015 Elsevier B.V. All rights reserved. C1 [Brevik, Eric C.] Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. [Richter, Daniel deB] Duke Univ, Nicholas Sch Environm, Durham, NC USA. [Verrecchia, Eric P.] Univ Lausanne, Fac Geosci & Environm, Inst Earth Surface Dynam, CH-1015 Lausanne, Switzerland. [Ryan, John] Carrigataha, Cahir, Tipperary, Ireland. [Ryan, John] Amer Univ Beirut, Beirut, Lebanon. [Ryan, John] ICARDA, Aleppo, Syria. [Poch, Rosa M.] Univ Lleida, Dept Medi Ambient & Ciencies Sol, Lleida 25198, Spain. [Crouvi, Onn] Geol Survey Israel, Jerusalem, Israel. [Sauer, Daniela] Univ Gottingen, Inst Geog, Gottingen, Germany. [Waroszewski, Jaroslaw] Wroclaw Univ Environm & Life Sci, Inst Soil Sci & Environm Protect, Wroclaw, Poland. [Solleiro-Rebolledo, Elizabeth] Univ Nacl Autonoma Mexico, Mexico City, DF, Mexico. [Monger, Curtis] USDA NRCS, Soil Sci Div, Lincoln, NE USA. [Monger, Curtis] New Mexico State Univ, Las Cruces, NM 88003 USA. [Ottner, Franz] Univ Nat Resources & Life Sci, Vienna Inst Appl Geol, Vienna, Austria. [Targulian, Victor] Russian Acad Sci, Inst Geog, Moscow, Russia. RP Brevik, EC (reprint author), Dickinson State Univ, Dept Nat Sci, Dickinson, ND 58601 USA. EM Eric.Brevik@dickinsonstate.edu; drichter@duke.edu; Eric.Verrecchia@unil.ch; ryanjohn1944@gmail.com; rosa.poch@macs.udl.cat; crouvi@gsi.gov.il; Daniela.Sauer@geo.uni-goettingen.de; jaroslaw.waroszewski@gmail.com; solleiro@geologia.unam.mx; Curtis.Monger@lin.usda.gov; franz.ottner@boku.ac.at; votargulian@gmail.com RI Poch, Rosa/O-2357-2014; Waroszewski, Jaroslaw/A-1300-2017 OI Poch, Rosa/0000-0001-8639-4204; NR 36 TC 2 Z9 2 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD NOV PY 2016 VL 146 BP 147 EP 154 DI 10.1016/j.catena.2015.10.012 PG 8 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DW8VK UT WOS:000383933400013 ER PT J AU Acebes-Doria, AL Leskey, TC Bergh, JC AF Acebes-Doria, Angelita L. Leskey, Tracy C. Bergh, J. Christopher TI Injury to apples and peaches at harvest from feeding by Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) nymphs early and late in the season SO CROP PROTECTION LA English DT Article DE Tree fruit; Brown marmorated stink bug; Malus domestica; Prunus persica ID MARMORATED STINK BUG; TREE FRUIT; HETEROPTERA; ORCHARDS; ATTRACTION; BEHAVIOR AB Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) is an invasive species that has become an important orchard pest in the Mid-Atlantic region of the US. Adults and nymphs feed on tree fruit. Feeding injury from adults has been characterized but the injury from nymphs has not been examined systematically. Since the four plant-feeding instars of H. halys (second through fifth) differ substantially in size, it is plausible that the effects of their feeding on fruit injury and injury expression may differ among them. We compared feeding injury at harvest from young nymphs (second plus third instars), older nymphs (fourth plus fifth instars), and adults that were caged on 'Smoothee Golden' apples and 'Redhaven' peaches in early June (peach and apple), late July (peach), and late August (apple). Individual apples and peaches were caged at fruit set and assigned to the following treatments (n = 28/treatment): 1) control (no H. halys), 2) young nymphs or 3) adults early in the season, and 4) young nymphs, 5) older nymphs or 6) adults later in the season. Fruit in each treatment were exposed to 3-4 young nymphs, two older nymphs or 1-2 adults placed in the cages for 96 h and evaluated for external and internal feeding injury within 36 h after harvest. No injury was recorded from unexposed peaches or apples. The percentage of injured fruit and number of injuries per fruit varied significantly among the exposed treatments. Early season feeding by young nymphs yielded the least injury to peaches and apples. In apples, the highest percentage of injured fruit and number of injuries per fruit were caused by late season feeding by adults. In peaches, early season adult feeding produced the highest percentage of injured fruit and injuries per fruit. More internal than external injury was recorded on peach and no such difference was observed on apple. The implications of these findings on H. halys management in fruit orchards are discussed. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Acebes-Doria, Angelita L.; Bergh, J. Christopher] Virginia Tech, Alson H Smith Jr Agr Res & Extens Ctr, Winchester, VA 22602 USA. [Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Acebes-Doria, AL (reprint author), Virginia Tech AHSAREC, 595 Laurel Grove Rd, Winchester, VA 22602 USA. EM aacebes@vt.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-51181-30937] FX We sincerely grateful to Jean Engelman, Eliezer Doria and the 2015 summer interns, Sophia Dutterer, Rachel Wilk, Jason Chou and Brendan Yarbrough for their excellent technical assistance. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number #2011-51181-30937. NR 27 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 58 EP 65 DI 10.1016/j.cropro.2016.06.022 PG 8 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400009 ER PT J AU Anderson, A Slootmaker, C Harper, E Holderieath, J Shwiff, SA AF Anderson, Aaron Slootmaker, Chris Harper, Erin Holderieath, Jason Shwiff, Stephanie A. TI Economic estimates of feral swine damage and control in 11 US states SO CROP PROTECTION LA English DT Article DE Feral swine; Invasive species; Crop damage; Economics; Survey ID CONSEQUENCES; RANGE AB We report the results of one of the most comprehensive surveys on feral swine (Sus scrofa) damage and control in 11 US states (Alabama, Arkansas, California, Florida, Georgia, Louisiana, Mississippi, Missouri, North Carolina, South Carolina and Texas). The survey was distributed by the USDA National Agricultural Statistical Service in the summer of 2015 to a sample of producers of corn (Zea mays), soybeans (Glycine max), wheat (Triticum), rice (Oryza sativa), peanuts (Arachis hypogaea), and sorghum (Sorghum bicolor) in the 11-state region. Producers that failed to respond to the initial mailing received multiple follow-up phone calls in an attempt to minimize non-response bias, and a total of 4377 responses were obtained. Findings indicate that damage can be substantial. The highest yield loss estimates occur in peanut and corn production in the Southeast and Texas. Control efforts are common, and producers incur considerable costs from shooting and trapping efforts. Extrapolating crop damage estimates to the state level in 10 states with reportable damage yields an estimated crop loss of $190 million. Though large, this number likely represents only a small fraction of the total damage by feral swine in the 10 states because it only includes crop damage to six crops. We hope findings from this survey will help guide control efforts and research, as well as serve as a benchmark against which the effectiveness of future control efforts can be measured. Published by Elsevier Ltd. C1 [Anderson, Aaron; Slootmaker, Chris; Harper, Erin; Holderieath, Jason; Shwiff, Stephanie A.] USDA APHIS WS, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. RP Anderson, A (reprint author), USDA APHIS WS, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM aaron.m.anderson@aphis.usda.gov NR 14 TC 0 Z9 0 U1 19 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 89 EP 94 DI 10.1016/j.cropro.2016.06.023 PG 6 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400013 ER PT J AU Accinelli, C Abbas, HK Little, NS Kotowicz, JK Mencarelli, M Shier, WT AF Accinelli, Cesare Abbas, Harried K. Little, Nathan S. Kotowicz, Jeremy K. Mencarelli, Mariangela Shier, W. Thomas TI A liquid bioplastic formulation for film coating of agronomic seeds SO CROP PROTECTION LA English DT Article DE Biodegradable plastic; Seed treatment; Maize; Oilseed rape; Pesticides; Trichoderma ID ASPERGILLUS-FLAVUS; TRICHODERMA-HARZIANUM; BIOCONTROL; GERMINATION; STRAIN; CORN AB Interest in industrial and domestic applications of biodegradable plastics from renewable sources is increasing, but their use in agriculture is still limited (e.g., mulching films, plant pots, and plant clips). However, a sprayable liquid bioplastic formulation was recently evaluated for application of microbial biocontrol agents to agricultural and horticultural crops. In this study bioplastic formulation has been evaluated for use hi film-coating seeds of two agronomic species, corn and canola. Bioplastic seed coating was achieved using procedures and equipment designed for commercial polymer film-coating of tablets. Germination of both species was unaffected by the thin bioplastic coating. Bioplastic coatings containing spores of the plant-growth promoting fungus, Trichoderma harzianum, significantly stimulated the growth of corn and canola seeds. In corn seedlings, shoot and root lengths were 29% and 44% longer, respectively, in seeds coated with T harzianum-containing bioplastic than in uncoated seeds. Similarly, in canola seedlings shoot and root lengths were 19% and 20% longer, respectively, in seeds coated with T harzianum-containing bioplastic than in uncoated seeds. In a paper-roll assay, including T harzianum spores prevented reduced germination caused by a mixture of the insecticide imidacloprid and the fungicide metalaxyl-M in bioplastic seed coatings. The adhesive and plastic properties of bioplastic reduced dust-off from bioplastic-coated seeds by 96% in corn and 99% in canola compared to seeds coated with a commercial polymer. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Accinelli, Cesare; Mencarelli, Mariangela] Alma Mater Studiorum Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. [Abbas, Harried K.; Kotowicz, Jeremy K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Little, Nathan S.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Shier, W. Thomas] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA. RP Accinelli, C (reprint author), Alma Mater Studiorum Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. EM cesare.accinelli@unibo.it NR 29 TC 0 Z9 0 U1 31 U2 31 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 123 EP 128 DI 10.1016/j.cropro.2016.07.010 PG 6 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400018 ER PT J AU Cai, H Wang, LC Yang, ZX Wan, QL Wei, W Davis, RE Zhao, Y AF Cai, H. Wang, L. C. Yang, Z. X. Wan, Q. L. Wei, W. Davis, R. E. Zhao, Y. TI Evidence for the role of an invasive weed in widespread occurrence of phytoplasma diseases in diverse vegetable crops: Implications from lineage-specific molecular markers SO CROP PROTECTION LA English DT Article DE Phytoplasma; Subgroup 16SrII-A; Parthenium weed; Immunodominant membrane protein ID 16S RIBOSOMAL-RNA; IMMUNODOMINANT MEMBRANE-PROTEIN; ONION YELLOWS PHYTOPLASMA; CANDIDATUS-PHYTOPLASMA; WITCHES-BROOM; RFLP ANALYSIS; GENES; AURANTIFOLIA; DETERMINES; MOLLICUTES AB During the period from 2011 to 2013, several plant diseases repeatedly occurred in vegetable crops grown in Yuanmou County, Yunnan Province, China. Affected plants included cowpea, sword bean, string bean, tomato, lettuce, and water spinach. The diseased plants exhibited symptoms of witches'-broom growth and floral deformations, linking each disease to phytoplasmal infection. Phylogenetic and virtual RFLP analyses of the phytoplasmal 16S rRNA gene sequences amplified from DNA of diseased plants revealed that all of the individual strains present in the diverse vegetable plants were affiliated with a single 'Candidatus Phytoplasma' species ('Ca. Phytoplasma aurantifolia') and a single ribosomal subgroup (16SrII-A). While presence of subgroup 16SrII-A phytoplasma in this geographic region was reported previously, such widespread infections in diverse plant hosts are unveiled for the first time in this study. In pursuing the source of the infections, we found that areas surrounding the affected vegetable fields were extensively invaded by parthenium weeds (Parthenium hysterophorus); and many of the weed plants exhibited abnormal morphologies that were suspicious of, and later diagnosed with, phytoplasmal infections. Results from genotyping of 16S rRNA and lineage-specific immunodominant membrane protein genes revealed that the vegetable-infecting phytoplasmas and the parthenium weed phytoplasma belong to the same genetic lineage. The findings indicate that parthenium weed poses a substantial risk as a reservoir of phytoplasmal infection of nearby agricultural crops in the geographic region since the ecosystems of Yuanmou are insect-rich, and parthenium weed is known to attract diverse leafhoppers. Further studies are warranted to assess the impact of farmland invasions by the noxious weed and to devise practical measures for improved weed control. Published by Elsevier Ltd. C1 [Cai, H.; Wan, Q. L.] Yunnan Agr Univ, Key Lab Plant Pathol, Kunming 650201, Peoples R China. [Wang, L. C.; Wan, Q. L.] Yuxi Normal Univ, Coll Resources & Environm, Yuxi 653100, Peoples R China. [Yang, Z. X.] Yunnan Acad Agr Sci, Inst Trop Ecoagr Sci, Yuanmou 651300, Peoples R China. [Wei, W.; Davis, R. E.; Zhao, Y.] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Zhao, Y (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov FU Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN [2014-2022]; National Natural Science Foundation of China [31060239]; U.S. Department of Agriculture, Agricultural Research Service [1245-22000-282-00D] FX This research was supported by the Program for Innovative Research Team (in Science and Technology) in University of Yunnan Province, IRTSTYN (2014-2022), the National Natural Science Foundation of China (Grant number 31060239) and the U.S. Department of Agriculture, Agricultural Research Service (Project number1245-22000-282-00D). NR 43 TC 0 Z9 0 U1 25 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD NOV PY 2016 VL 89 BP 193 EP 201 DI 10.1016/j.cropro.2016.07.025 PG 9 WC Agronomy SC Agriculture GA DW7JG UT WOS:000383826400026 ER PT J AU Maffei, HM Filip, GM Grulke, NE Oblinger, BW Margolis, EQ Chadwick, KL AF Maffei, Helen M. Filip, Gregory M. Grulke, Nancy E. Oblinger, Brent W. Margolis, Ellis Q. Chadwick, Kristen L. TI Pruning high-value Douglas-fir can reduce dwarf mistletoe severity and increase longevity in Central Oregon SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pseudotsuga menziesii var. menziesii; Arceuthobium douglasii; Pruning; Tree rings; Normalized Difference Vegetation Index (NDVI); Oregon ID WESTERN SPRUCE BUDWORM; GROWTH; ARCEUTHOBIUM; MORTALITY; LEAF AB Mid- to very large-sized Douglas-fir (Pseudotsuga menzieseii var. menziesii) that were lightly- to moderately-infected by dwarf mistletoe (Arceuthobium douglasii) were analyzed over a 14-year period to evaluate whether mechanical pruning could eradicate mistletoe (or at least delay the onset of severe infection) without significantly affecting tree vitality and by inference, longevity. Immediate and long-term pruning effects on mistletoe infection severity were assessed by comparing pruned trees (n = 173) to unpruned trees (n = 55) with respect to: (1) percentage of trees with no visible infections 14 years post-pruning, (2) Broom Volume Rating (BVR), and (3) rate of BVR increase 14 years post pruning. Vitality/longevity (compared with unpruned trees) was assessed using six indicators: (1) tree survival, (2) the development of severe infections, (3) the development of dead tops, (4) tree-ring width indices, (5) Normalized Difference Vegetation Index (NDVI) from high-resolution multi-spectral imagery, and (6) live-crown ratio (LCR) and increment. Twenty-four percent of the pruned trees remained free of mistletoe 14 years post-pruning. Pruning is most likely to successfully eradicate mistletoe in lightly infected trees (BVR 1 or 2) without infected neighbors. Pruning significantly decreased mean BVR in the pruned versus the unpruned trees. However, the subsequent average rate of intensification (1.3-1.5 BVR per decade) was not affected, implying that a single pruning provides 14 years respite in the progression of infection levels. Post-pruning infection intensification was slower on dominant and co-dominants than on intermediate or suppressed trees. The success of mistletoe eradication via pruning and need for follow-up pruning should be evaluated no sooner than 14 years after pruning to allow for the development of detectable brooms. Based on six indicators, foliage from witches brooms contribute little to long-term tree vitality since removal appears to have little effect on resources available for tree growth and maintenance. In the severely pruned trees, tree-ring width was reduced for several years post-pruning, but then compensated with larger ring width in later years. Both NDVI and LCR increment were significantly higher for the pruned trees than the control trees, while the development of severe infections and/or dead tops was significantly (5x and 3 x) higher for the controls. If possible, multiple indicators of tree vitality should be evaluated. Pruning can be worthwhile even if all the mistletoe is not removed, because mistletoe intensification is delayed. The impact of removing the brooms seems to be minimal, and post-pruning crowns had greater NDVI values. Published by Elsevier B.V. C1 [Maffei, Helen M.; Oblinger, Brent W.] US Forest Serv, USDA, 63095 Deschutes Market Rd, Bend, OR 97701 USA. [Filip, Gregory M.] US Forest Serv, USDA, Pacific Northwest Reg Off, 1220 SW Third Ave, Portland, OR 97204 USA. [Grulke, Nancy E.] US Forest Serv, USDA, Western Wildland Environm Threat Assessment Ctr, 3160 NE 3rd St, Prineville, OR 97754 USA. [Margolis, Ellis Q.] US Geol Survey, Ft Collins Sci Ctr, Jemez Mt Field Stn, 301 Dinosaur Trail, Santa Fe, NM 87508 USA. [Chadwick, Kristen L.] US Forest Serv, USDA, 16400 Champion Way, Sandy, OR 97055 USA. RP Maffei, HM (reprint author), US Forest Serv, USDA, 63095 Deschutes Market Rd, Bend, OR 97701 USA. EM hmaffei@fs.fed.us NR 35 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 11 EP 19 DI 10.1016/j.foreco.2016.07.014 PG 9 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600002 ER PT J AU Bradley, JC Will, RE Stewart, JF Nelson, CD Guldin, JM AF Bradley, J. C. Will, R. E. Stewart, J. F. Nelson, C. D. Guldin, J. M. TI Post-fire resprouting of shortleaf pine is facilitated by a morphological trait but fire eliminates shortleaf x loblolly pine hybrid seedlings SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Basal crook; Fire; Pinus echinata; Pinus taeda; Resprouting; Shortleaf x loblolly pine hybrid ID SHAPING PLANT TRAITS; CLIMATE AB Concurrent with fire exclusion, shortleaf pine x loblolly pine hybrids have increased throughout the southeastern USA and may threaten the genetic integrity of shortleaf pine. Historically, fire favored short leaf pine over other southern pine species, especially loblolly pine, with which it shares a broad sympatric natural range. Shortleaf pine seedlings have a morphological adaptation (basal crook) that lowers dormant buds to the soil surface where they are presumably protected from fire to facilitate resprouting after topkill. To evaluate this presumption, we tested (1) the functional role of the basal crook in protecting dormant buds by measuring resprouting after exposing the lower stem of shortleaf pine to fire and protecting from fire the lower stem (and dormant buds) of loblolly pine (which lacks a basal crook) and (2) the occurrence and importance of the basal crook morphological adaptation in Fl shortleaf x loblolly pine seedlings by comparing morphology and post-fire resprouting of the hybrids to both parents. Fire exposure of shortleaf pine seedling dormant buds caused seedling mortality, while protecting loblolly pine dormant buds facilitated resprouting. Hybrid pines have basal crooks intermediate to the strong crook of shortleaf pine and loblolly pine's non-crooked stem. Fire top-killed loblolly pine and short leaf x loblolly pine seedlings, and they did not resprout, while 57% of shortleaf pine resprouted after top kill during two dormant season and one growing season burns. This highly significant difference shows that the basal crook is important for shortleaf pine resprouting after topkill by fire. Fire is an important tool to reduce shortleaf x loblolly pine hybrids, and to provide a competitive advantage to shortleaf pine relative to loblolly pine, for ecosystem restoration and maintaining the genetic integrity of shortleaf pine. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bradley, J. C.; Will, R. E.] Oklahoma State Univ, Dept Nat Resources Ecol & Management, 008C Agr Hall, Stillwater, OK 74078 USA. [Stewart, J. F.] Oklahoma State Univ, Dept Integrat Biol, Stillwater, OK 74078 USA. [Nelson, C. D.] US Forest Serv, USDA, Southern Res Stn, Southern Inst Forest Genet, 23332 Success Rd, Saucier, MS 39574 USA. [Nelson, C. D.] Univ Kentucky, Forest Hlth Res & Educ Ctr, 208 TP Cooper Hall, Lexington, KY 40546 USA. [Guldin, J. M.] US Forest Serv, USDA, Southern Res Stn, 607 Reserve St, Hot Springs, AR 71902 USA. RP Will, RE (reprint author), Oklahoma State Univ, Dept Nat Resources Ecol & Management, 008C Agr Hall, Stillwater, OK 74078 USA. EM rodney.will@okstate.edu RI Will, Rodney/G-8111-2011 FU Oklahoma Agricultural Experimental Station; McIntire-Stennis project [OKL0 2929]; USDA Forest Service Cooperative Agreement [14-CA-11330126-104] FX We would like to thank the Oklahoma Agricultural Experimental Station, McIntire-Stennis project OKL0 2929, and USDA Forest Service Cooperative Agreement 14-CA-11330126-104 for providing funding for this research. We are deeply grateful for the assistance from Bob Heinemann and the staff at the Kiamichi Forestry Research Center, including Cassandra Meek, Randy Holeman, Dennis Wilson, Glenda Robinson, and Keith Anderson. We would also like to thank Professor Emeritus Charles Tauer for making the shortleaf x loblolly crosses and initiating this line of research. We also appreciated the help of Duncan Wilson, Adam Maggard, Dipesh KC, Lays Francisco, Rodrigo Carvalhais, and Casey Ausmus at various crucial points NR 26 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 146 EP 152 DI 10.1016/j.foreco.2016.08.016 PG 7 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600015 ER PT J AU Moser, WK Fan, ZF Hansen, MH Crosby, MK Fan, SX AF Moser, W. Keith Fan, Zhaofei Hansen, Mark H. Crosby, Michael K. Fan, Shirley X. TI Invasibility of three major non-native invasive shrubs and associated factors in Upper Midwest US forest lands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Invasion potential; Exotic species; FIA ID PLANT INVASIONS; UNITED-STATES; PATTERNS; CONSERVATION; MANAGEMENT AB We used non-native invasive plant data from the US Forest Service's Forest Inventory and Analysis (FIA) program, spatial statistical methods, and the space (cover class)-for-time approach to quantify the invasion potential and success ("invasibility") of three major invasive shrubs (multiflora rose, non-native bush honeysuckles, and common buckthorn) in broadly classified forest-type gtoups in seven Upper Midwest states. Smoothed maps of presence and cover percent showed a strong clustering pattern for all three invasive shrubs despite their different ranges. The species are clustered around major cities or urban areas (e.g., Chicago, Illinois, and Des Moines, Iowa), indicating the potential role humans played in their invasion and spread on the landscape and throughout the Midwest. Conditional inference tree (ctree) models further quantified the significant factors contributing to the observed regional patterns: for distribution of multiflora rose, percentage of forest cover in the county (measuring human disturbance intensity) and stand density index; for distribution of common buckthorn, distance to major highways. Non-native bush honeysuckles were not associated with any disturbance and site/stand variables except for latitude and longitude. The infested FIA plots by cover class were positively associated in space, signifying a concentric-like spread trend from previously infested sites (hot spots) to surrounding areas. By forest-type groups or as a whole, the three species spread slowly at earlier stages, but recently have increased significantly in presence/expansion. Oak/hickory and elm/ash/cottonwood forests were more susceptible to the three invasive shrubs compared to other forests. We recommend that resource managers and planners prescribe control and mitigation treatments for non-native invasive plants by forest types and spatial locations close to highways and residences. Published by Elsevier B.V. C1 [Moser, W. Keith] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Fan, Zhaofei; Fan, Shirley X.] Auburn Univ, Auburn, AL 36849 USA. [Crosby, Michael K.] Shorter Univ, Rome, GA USA. [Hansen, Mark H.] US Forest Serv, USDA, Northern Res Stn, St Paul, MN USA. RP Moser, WK (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. EM wkmoser@fs.fed.us FU US Forest Service, Forest Health Protection, Environmental Monitoring program; US Forest Service; Northern Research Station; Forest Inventory and Analysis program; Mississippi State University; Department of Forestry FX The authors thank the US Forest Service, Forest Health Protection, Environmental Monitoring program; the US Forest Service, Northern Research Station, Forest Inventory and Analysis program; and the Mississippi State University, Department of Forestry for their support of this study. Dr. Mark D. Nelson, US Forest Service, Northern Research Station, Forest Inventory and Analysis program, provided valuable assistance with spatially joining FIA plots and other features. Benjamin Bird, Cynthia Moser and two anonymous reviewers of earlier versions of this manuscript offered valuable comments. NR 40 TC 0 Z9 0 U1 67 U2 67 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 195 EP 205 DI 10.1016/j.foreco.2016.07.043 PG 11 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600020 ER PT J AU Bell, DM Gray, AN AF Bell, David M. Gray, Andrew N. TI Assessing intra- and inter-regional climate effects on Douglas-fir biomass dynamics in Oregon and Washington, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Climate; Douglas-fir; Forest inventory; Forest structure; Forest succession; Pacific Northwest; Pseudotsuga menziesii ID NEAREST-NEIGHBOR IMPUTATION; PACIFIC-NORTHWEST; UNITED-STATES; FOREST; COMMUNITIES; SUCCESSION; MODELS; GROWTH; DISTURBANCES; ECOSYSTEMS AB While ecological succession shapes contemporary forest structure and dynamics, other factors like forest structure (dense vs. sparse canopies) and climate may alter structural trajectories. To assess potential sources of variation in structural trajectories, we examined proportional biomass change for a regionally dominant tree species, Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), across vegetation zones representing broad gradients in precipitation and temperature with 3510 forest inventory plots in Oregon and Washington, USA. We found that P. menziesii biomass change decreased with P. menziesii biomass stocks and increased with P. menziesii density, remaining positive in older stands only in the wet and warm vegetation zone. Within two of the vegetation zones, biomass change was greatest in warm and wet environments. In dry vegetation zones, positive P. menziesii biomass change responses to initial canopy cover and canopy cover change (i.e., increases with cover loss and decreases with cover gain) indicated shifts in forest structure. Variation in P. menziesii biomass dynamics within and between vegetation zones imply multi-scale climatic controls on forest structural trajectories for P. menziesii and highlight the potential for continued atmospheric carbon sequestration in warm and wet forests of the Pacific Northwest for both young and old forests, given that future climatic conditions support similar forest dynamics. Published by Elsevier B.V. C1 [Bell, David M.; Gray, Andrew N.] US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Bell, DM (reprint author), 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dmbell@fs.fed.us; agray01@fs.fed.us FU USDA Forest Service Pacific Northwest Research Station FX This research was funded by the USDA Forest Service Pacific Northwest Research Station. We thank Matthew Gregory for extracting canopy cover estimates for each plot from the GNN data. We thank Matthew Reilly, Dan Binkley, and two anonymous reviewers for providing comments on the manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the United States government. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the USDA Forest Service. NR 46 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD NOV 1 PY 2016 VL 379 BP 281 EP 287 DI 10.1016/j.foreco.2016.07.023 PG 7 WC Forestry SC Forestry GA DW7FM UT WOS:000383816600029 ER PT J AU Yen, H White, MJ Arnold, JG Keitzer, SC Johnson, MVV Atwood, JD Daggupati, P Herbert, ME Sowa, SP Ludsin, SA Robertson, DM Srinivasan, R Rewa, CA AF Yen, Haw White, Michael J. Arnold, Jeffrey G. Keitzer, S. Conor Johnson, Mari-Vaughn V. Atwood, Jay D. Daggupati, Prasad Herbert, Matthew E. Sowa, Scott P. Ludsin, Stuart A. Robertson, Dale M. Srinivasan, Raghavan Rewa, Charles A. TI Western Lake Erie Basin: Soft-data-constrained, NHDPIus resolution watershed modeling and exploration of applicable conservation scenarios SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Lake Erie; NHDPIus model; Soft data; Conservation practice; Swat-SAS; Model calibration ID DEPOSITED FINE SEDIMENT; FRESH-WATER; SWAT MODEL; INPUT DATA; MANAGEMENT-PRACTICES; DISSOLVED NUTRIENTS; MULTIPLE STRESSORS; OUTPUT UNCERTAINTY; MISSISSIPPI RIVER; ASSESSMENT-TOOL AB Complex watershed simulation models are powerful tools that can help scientists and policy-makers address challenging topics, such as land use management and water security. In the Western Lake Erie Basin (WLEB), complex hydrological models have been applied at various scales W help describe relationships between land use and water, nutrient, and sediment dynamics. This manuscript evaluated the capacity of the current Soil and Water Assessment Tool (SWAT) to predict hydrological and water quality processes within WLEB at the finest resolution watershed boundary unit (NHDPIus) along with the current conditions and conservation scenarios. The process based SWAT model was capable of the fine-scale computation and complex routing used in this project, as indicated by measured data at five gaging stations. The level of detail required for fine-scale spatial simulation made the use of both hard and soft data necessary in model calibration, alongside other model adaptations. Limitations to the model's predictive capacity were due to a paucity of data in the region at the NHDPIus scale rather than due to SWAT functionality. Results of treatment scenarios demonstrate variable effects of structural practices and nutrient management on sediment and nutrient loss dynamics. Targeting treatment to acres with critical outstanding conservation needs provides the largest return on investment in terms of nutrient loss reduction per dollar spent, relative to treating acres with lower inherent nutrient loss vulnerabilities. Importantly, this research raises considerations about use of models to guide land management decisions at very fine spatial scales. Decision makers using these results should be aware of data limitations that hinder fine-scale model interpretation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Yen, Haw] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [White, Michael J.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Keitzer, S. Conor; Ludsin, Stuart A.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Aquat Ecol Lab, 1314 Kinnear Rd, Columbus, OH 43212 USA. [Johnson, Mari-Vaughn V.; Atwood, Jay D.] USDA, Grassland Soil & Water Res Lab, Resources Assessment Div, NRCS, 808 East Blackland Rd, Temple, TX 76502 USA. [Daggupati, Prasad; Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, Suite 221,1500 Res Plaza, College Stn, TX 77843 USA. [Herbert, Matthew E.; Sowa, Scott P.] Michigan Field Off, Nat Conservancy, 101 East Grand River Ave, Lansing, MI 48906 USA. [Robertson, Dale M.] US Geol Survey, Wisconsin Water Sci Ctr, 8505 Res Way, Middleton, WI 53562 USA. [Rewa, Charles A.] USDA, Resources Assessment Div, NRCS, George Washington Carver Ctr, Beltsville, MD 20705 USA. RP Yen, H (reprint author), Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com RI Srinivasan, R/D-3937-2009; Daggupati, Prasad/D-8886-2017 OI Daggupati, Prasad/0000-0002-7044-3435 FU United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland [68-7482-12-504] FX This project was funded by grants from the United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components (Cooperative Agreement: 68-7482-12-504). USDA is an equal opportunity employer and provider! NR 93 TC 4 Z9 4 U1 24 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD NOV 1 PY 2016 VL 569 BP 1265 EP 1281 DI 10.1016/j.scitotenv.2016.06.202 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU5RM UT WOS:000382269000122 PM 27387796 ER PT J AU Lichtenberg, E Page, ET AF Lichtenberg, Erik Page, Elina Tselepidakis TI Prevalence and cost of on-farm produce safety measures in the Mid-Atlantic SO FOOD CONTROL LA English DT Article DE Food safety; Produce safety; Food safety modernization act; Tomatoes; Leafy greens; Compliance cost; Regulatory burden; Mid-Atlantic; Vegetable growers ID GOOD AGRICULTURAL PRACTICES; FOOD; SCALE AB We use data from a survey of leafy green and tomato growers in the Mid-Atlantic region to investigate the prevalence and cost of produce safety practices required under the Produce Rule of the Food Safety Modernization Act. Majorities of our respondents currently employ most of the food safety practices that will be required under the Produce Rule. But the Produce Rule will nevertheless require changes on the part of some growers. We find that farm size has a statistically and quantitatively negligible relationship with the use of most produce safety practices except for the sampling and testing of water, soil amendments, and product samples. Contrary to the theoretical literature suggesting that traceability increases incentives to take precautionary measures, we find little evidence that the use of produce safety practices is correlated with any marketing channel. We do find that all of these practices exhibit substantial increasing returns to scale, implying that the burden of complying with the provisions of the Produce Rule is much lower for large operations than small ones. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Lichtenberg, Erik] Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. [Page, Elina Tselepidakis] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Lichtenberg, E (reprint author), Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. EM elichten@umd.edu OI Lichtenberg, Erik/0000-0003-0619-6254 FU United States Department of Agriculture/National Institute of Food and Agriculture Specialty Crop Research Initiative Grant [2011-5118130767] FX This research was supported by United States Department of Agriculture/National Institute of Food and Agriculture Specialty Crop Research Initiative Grant 2011-5118130767 to the University of Maryland, College Park. The views expressed are the authors' and may not be attributed to the Economic Research Service or the U.S. Department of Agriculture. NR 16 TC 0 Z9 0 U1 4 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD NOV PY 2016 VL 69 BP 315 EP 323 DI 10.1016/j.foodcont.2016.04.054 PG 9 WC Food Science & Technology SC Food Science & Technology GA DQ5XM UT WOS:000379278600042 ER PT J AU Cabanillas, B Crespo, JF Maleki, SJ Rodriguez, J Novak, N AF Cabanillas, Beatriz Crespo, Jesus F. Maleki, Soheila J. Rodriguez, Julia Novak, Natalija TI Pin p 1 is a major allergen in pine nut and the first food allergen described in the plant group of gymnosperms SO FOOD CHEMISTRY LA English DT Article DE Albumin; Basophil activation test; Pin p 1; Pine nut allergy; Tree nut allergy ID PROTEINS; PEPTIDES; PEANUT AB This study aimed to report the complete sequence of a 2S albumin purified from pine nut and to analyze its allergenic properties. Individual recognition of this protein by serum IgE from pine nut-allergic patients was assessed. IgE cross-linking capacity was analyzed in a basophil activation test. Inhibition of IgE-binding and stability to heating was also assessed. The complete nucleotide sequence was obtained and a phylogenetic study was carried out. 2S albumin from pine nut (registered as Pin p 1.0101) was recognized by IgE of 75% of sera. The allergen was heat-stable and had a robust capacity to inhibit IgEbinding to whole pine nut extract. The IgE cross-linking capacity of Pin p 1 on basophils was also demonstrated. Despite the low homology of Pin p 1 sequence with other allergenic 2S albumins from angiosperms, Pin p 1 contains the typical skeleton of 8 cysteine residues, important for its a-helixes enriched structure. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cabanillas, Beatriz; Novak, Natalija] Univ Bonn, Med Ctr, Dept Dermatol & Allergy, Sigmund Freud Str 25, D-53127 Bonn, Germany. [Crespo, Jesus F.; Rodriguez, Julia] Hosp Univ 12 Octubre, Serv Alergia, Inst Invest Hosp 12 Octubre I 12, Ave Cordoba S-N, Madrid 28041, Spain. [Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA USA. RP Cabanillas, B (reprint author), Univ Bonn, Med Ctr, Dept Dermatol & Allergy, Sigmund Freud Str 25, D-53127 Bonn, Germany. EM Beatriz.Cabanillas@ukb.uni-bonn.de FU Cluster of Excellence ImmunoSensation of the German Research Foundation (DFG) (Germany); Christine Kuhne Stiftung CK-CARE; BONFOR Grant of the University of Bonn (Germany) FX The study was supported by Cluster of Excellence ImmunoSensation of the German Research Foundation (DFG) (Germany), the Christine Kuhne Stiftung CK-CARE and a BONFOR Grant of the University of Bonn (Germany). NR 20 TC 0 Z9 0 U1 18 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 1 PY 2016 VL 210 BP 70 EP 77 DI 10.1016/j.foodchem.2016.04.068 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DM2FT UT WOS:000376163100010 PM 27211622 ER PT J AU Qin, YL Qiu, XQ Zhu, JY AF Qin, Yanlin Qiu, Xueqing Zhu, J. Y. TI Understanding Longitudinal Wood Fiber Ultra-structure for Producing Cellulose Nanofibrils Using Disk Milling with Diluted Acid Prehydrolysis SO SCIENTIFIC REPORTS LA English DT Article ID ENZYMATIC SACCHARIFICATION; MICROFIBRILLATED CELLULOSE; ENDOGLUCANASE TREATMENT; TRANSPARENT FILMS; LODGEPOLE PINE; HYDROLYSIS; PULP; MICROFLUIDIZATION; HOMOGENIZATION; LIGNOCELLULOSE AB Here we used dilute oxalic acid to pretreat a kraft bleached Eucalyptus pulp (BEP) fibers to facilitate mechanical fibrillation in producing cellulose nanofibrils using disk milling with substantial mechanical energy savings. We successfully applied a reaction kinetics based combined hydrolysis factor (CHFX) as a severity factor to quantitatively control xylan dissolution and BEP fibril deploymerization. More importantly, we were able to accurately predict the degree of polymerization (DP) of disk-milled fibrils using CHFX and milling time or milling energy consumption. Experimentally determined ratio of fibril DP and number mean fibril height (diameter d), DP/d, an aspect ratio measurer, were independent of the processing conditions. Therefore, we hypothesize that cellulose have a longitudinal hierarchical structure as in the lateral direction. Acid hydrolysis and milling did not substantially cut the "natural" chain length of cellulose fibrils. This cellulose longitudinal hierarchical model provides support for using weak acid hydrolysis in the production of cellulose nanofibrils with substantially reduced energy input without negatively affecting fibril mechanical strength. C1 [Qin, Yanlin] Guangdong Univ Technol, Sch Chem Engn & Light Ind, Guangzhou, Guangdong, Peoples R China. [Qiu, Xueqing] South China Univ Technol, Sch Chem & Chem Engn, Guangzhou, Guangdong, Peoples R China. [Qin, Yanlin; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; Chinese Scholarship Council (CSC); International Science and Technology Cooperation Program of China (ISTCP) [2013DFA41670]; National Natural Science Foundation of China [21576106] FX Financial support for this work included USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), and the International Science and Technology Cooperation Program of China (ISTCP): 2013DFA41670, National Natural Science Foundation of China (21576106). We also acknowledge Ms. Lixuan Yu, a graduate student at South China University for her help on some AFM imaging work. NR 45 TC 2 Z9 2 U1 7 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 31 PY 2016 VL 6 AR 35602 DI 10.1038/srep35602 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA3TD UT WOS:000386526900001 PM 27796325 ER PT J AU Wang, J Peng, YD He, C Wei, BY Liang, YS Yang, HL Wang, Z Stanley, D Song, QS AF Wang, Juan Peng, Yuan-De He, Chao Wei, Bao-Yang Liang, Yun-Shan Yang, Hui-Lin Wang, Zhi Stanley, David Song, Qi-Sheng TI Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata SO GENE LA English DT Article DE Cry1Ab protein; Vitellin; Transcriptome; P. pseudoannulata ID NILAPARVATA-LUGENS STAL; INDICA BASMATI RICE; TRANSGENIC BT RICE; CHRYSOPERLA-SINICA; BROWN PLANTHOPPER; FIELD-EVALUATION; RNA-SEQ; PROTEIN; TOOL; RESISTANCE AB The impact of Bacillus thuringiensis (Bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. Here, we investigated the potential effects of Cry1Ab expressing rice on fecundity of the wolf spider, Pardosa pseudoannulata, and some of the underlying molecular mechanisms. The results indicated that brown planthoppers (BPHs) reared on Cry1Ab-expressing rice accumulated the Cry toxin and that reproductive parameters (pre-oviposition period, post-oviposition stage, number of eggs, and egg hatching rate) of the spiders that consumed BPHs reared on Bt rice were not different from those that consumed BPHs reared on the non-Bt control rice. The accumulated Cry1Ab did not influence several vitellin (Vt) parameters, including stored energy and amino acid composition, during one generation. We considered the possibility that the Cry toxins exert their influence on beneficial predators via more subtle effects detectable at the molecular level in terms of gene expression. This led us to transcriptome analysis to detect differentially expressed genes in the ovaries of spiders exposed to dietary Cry1Ab and their counterpart control spiders. Eight genes, associated with vitellogenesis, vitellogenin receptor activity, and vitellin membrane formation were not differentially expressed between ovaries from the treated and control spiders, confirmed by qPCR analysis. We infer that dietary Cry1Ab expressing rice does not influence fecundity, nor expression levels of Vt-associated genes in P. pseudoannulata. (C) 2016 Published by Elsevier B.V. C1 [Wang, Juan; Wei, Bao-Yang; Liang, Yun-Shan; Yang, Hui-Lin; Wang, Zhi] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China. [Peng, Yuan-De] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China. [He, Chao] Cent S Univ, Longping Coll, Changsha 410001, Hunan, Peoples R China. [Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Song, Qi-Sheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Wang, Z (reprint author), Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China. EM wangzhisipder@hotmail.com; SongQ@missouri.edu FU National Natural Science Foundation of China [31071943, 31272339]; Science Research Fund of the Education Department of Hunan Province [10 A054]; Agricultural Science and Technology Innovation Program of China [CAAS-ASTIP-2016-IBFC] FX This work was supported by the National Natural Science Foundation of China (No. 31071943, 31272339), the Science Research Fund of the Education Department of Hunan Province (No. 10 A054), and the Agricultural Science and Technology Innovation Program of China (No. CAAS-ASTIP-2016-IBFC). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 43 TC 0 Z9 0 U1 22 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD OCT 30 PY 2016 VL 592 IS 1 BP 1 EP 7 DI 10.1016/j.gene.2016.07.041 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA DW0AZ UT WOS:000383304600001 PM 27452121 ER PT J AU Oblath, EA Isbell, TA Berhow, MA Allen, B Archer, D Brown, J Gesch, RW Hatfield, JL Jabro, JD Kiniry, JR Long, DS AF Oblath, Emily A. Isbell, Terry A. Berhow, Mark A. Allen, Brett Archer, David Brown, Jack Gesch, Russell W. Hatfield, Jerry L. Jabro, Jalal D. Kiniry, James R. Long, Daniel S. TI Development of near-infrared spectroscopy calibrations to measure quality characteristics in intact Brassicaceae germplasm SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Brassicaceae; Near-infrared spectroscopy; Intact seed; Oil; Total glucosinolates; Chlorophyll ID FATTY-ACID-COMPOSITION; REFLECTANCE SPECTROSCOPY; OIL CONTENT; RAPESEED QUALITY; SINGLE SEEDS; NAPUS L.; GLUCOSINOLATE; PROTEIN; CROPS; COMPONENTS AB Determining seed quality parameters is an integral part of cultivar improvement and germplasm screening. However, quality tests are often time consuming, seed destructive, and can require large seed samples. This study describes the development of near-infrared spectroscopy (NIRS) calibrations to measure moisture, oil, fatty acid profile, nitrogen, glucosinolate, and chlorophyll content in six species from the Brassicaceae family. Rapeseed and similar oilseeds are potential feedstocks for producing hydrotreated renewable jet fuel. Screening samples with NIRS would allow cultivars with desirable characteristics to be quickly identified. A total of 367 samples of six species (Brassica napus, Brassica carinata, Brassica juncea, Brassica rapa, Sinapis alba, and Camelina saliva) were scanned with NIRS. Global calibrations for all six species were developed using modified partial least squares regression with reference values obtained through wet chemistry techniques. Comparing predicted values to reference data, the coefficients of determination (r(2)) and ratios of performance to deviation (RPD) varied, with some calibrations performing better than others. The calibration equations for seed oil content (r(2) = 0.98, RPD = 7.3) and nitrogen (r(2) = 0.98, RPD = 5.3) performed very well while the equations for seed moisture (r(2) = 0.93, RPD = 3.8) and total glucosinolate content (r(2) = 0.92, RPD = 2.3) were more qualitative. Large variation was observed for chlorophyll content (0-390 mg/kg) so two calibration equations were developed, one for the higher and one for the lower range of values. When combined, these calibrations also showed very good performance (r(2) = 0.99, RPD = 14). The performance of the calibrations for the fatty acids was more varied, with some performing very well, such as the calibration for C-18:3 (r(2) = 0.99, RPD = 9.9), and others, such as C-22:0 (r(2) = 0.69, RPD = 1.9), showing poor correlation. (C) 2016 Published by Elsevier B.V. C1 [Oblath, Emily A.; Isbell, Terry A.; Berhow, Mark A.] USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. [Allen, Brett; Jabro, Jalal D.] USDA ARS, NPARL, Sidney, MT 59270 USA. [Archer, David] USDA ARS, NGPRL, Mandan, ND 58554 USA. [Brown, Jack] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. [Gesch, Russell W.] USDA ARS, NCSCRL, Morris, MN 56267 USA. [Hatfield, Jerry L.] USDA ARS, NLAE, Ames, IA 50011 USA. [Kiniry, James R.] USDA ARS, GSWRL, Temple, TX 76502 USA. [Long, Daniel S.] USDA ARS, CPCRC, Pendleton, OR 97801 USA. RP Oblath, EA (reprint author), USDA ARS, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM emily.oblath@ars.usda.gov; terry.isbell@ars.usda.gov; mark.berhow@ars.usda.gov; brett.allen@ars.usda.gov; david.archer@ars.usda.gov; jbrown@uidaho.edu; russ.gesch@ars.usda.gov; jerry.hatfield@ars.usda.gov; jay.jabro@ars.usda.gov; jim.kiniry@ars.usda.gov; dan.long@ars.usda.gov FU USDA National Institute of Food and Agriculture (NIFA) [2012-10008-19727] FX The authors would like to thank Linda K. Manthey for technical assistance. This work was supported by Research Grant Award No. 2012-10008-19727 from the USDA National Institute of Food and Agriculture (NIFA). Seed for B. carinata genotypes was provided under an unfunded cooperative agreement with Agrisoma Biosciences, Inc. NR 34 TC 0 Z9 0 U1 18 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 52 EP 58 DI 10.1016/j.indcrop.2016.03.022 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000008 ER PT J AU Hagler, JR Tassone, EE Coffelt, TA Lipka, AE AF Hagler, James R. Tassone, Erica E. Coffelt, Terry A. Lipka, Alexander E. TI Host plant preference of Lygus hesperus exposed to three desert-adapted industrial crops SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Centrapalus pauciflorus; Parthenium argretatum; Physaria fendleri; Choice test; Oviposition preference; Feeding preference ID ALFALFA TRAP CROPS; ORGANIC STRAWBERRIES; VERNONIA-GALAMENSIS; MIRIDAE; COTTON; HEMIPTERA; BUG; HETEROPTERA; ABSCISSION; MOVEMENT AB The desert-adapted crops vernonia (Centrapalus pauciflorus), lesquerella (Physaria fendleri), and camelina (Camelina sativa) are being grown in the arid southwestern USA as potential feedstock for biofuel and/or other environmentally friendly products. A plant feeding choice test was conducted to determine the relative attractiveness of these three "new" crops to a possible insect pest, Lygus hesperus Knight. Adult L. hesperus were readily observed feeding or resting on the flowering structures of each plant type, but they were seen most often on vernonia and least often on camelina. Lygus hesperus readily deposited their eggs on each plant species, but again, the greatest amount of egg deposition was found on vernonia and the least on camelina. These studies indicate that L. hesperus might pose a threat to the production of these new crops. Moreover, the commercial expansion of these crops could significantly alter the population dynamics of the existing arthropod community. New challenges for managing this pest during regional crop production changes are discussed. Published by Elsevier B.V. C1 [Hagler, James R.; Tassone, Erica E.; Coffelt, Terry A.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hagler, JR (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov NR 31 TC 0 Z9 0 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 363 EP 367 DI 10.1016/j.indcrop.2016.05.041 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000046 ER PT J AU Schillinger, WF Werner, SJ AF Schillinger, William F. Werner, Scott J. TI Horned lark damage to pre-emerged canola seedlings SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biodiesel; Eremophilia alperestis; Horned lark; Canola seedlings; Crop damage ID CROPS AB Winter canola (Brassica napus L.) is considered the most promising domestically-produced oilseed feedstock for biodiesel production and for diversifying wheat (Triticum aestivum L.)-based cropping systems in the Inland Pacific Northwest, USA. Winter canola field experiments conducted in east-central Washington were completely destroyed, and commercial fields were damaged or destroyed, over several years by large flocks of horned larks (Eremophilia alperestis L) that ate the cotyledon leaves of pre-emerged and newly-emerged seedlings. Numerous control methods were attempted in field experiments, including laying bird netting over the entire experiment, placement of a life-size predator decoy in a field experiment, loud propane-powered cannon blasts, and mixing garlic with canola seed before planting followed by spraying garlic water on the soil surface. None of the attempted control methods were successful. This is the first report in the literature of horned lark damage to pre-emerged and newly-emerged canola seedlings. We discuss questions relevant to our novel account as well as potential abatement using falcons and non-toxic chemical repellents for the protection of industrial canola crops associated with horned lark depredation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Schillinger, William F.] Washington State Univ, Dept Crop & Soil Sci, Dryland Res Stn, 781 E Expt Stn Rd, Lind, WA 99341 USA. [Werner, Scott J.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Schillinger, WF (reprint author), Washington State Univ, Dept Crop & Soil Sci, Dryland Res Stn, 781 E Expt Stn Rd, Lind, WA 99341 USA. EM william.schillinger@wsu.edu FU WSU Agricultural Research Center through Hatch Project [0250]; Washington State Legislature FX Support was provided by the WSU Agricultural Research Center through Hatch Project 0250 and by the WSU Oilseed Cropping Systems Project through direct funding from the Washington State Legislature. The authors thank WSU research technicians John Jacobsen, Bruce Sauer, Steve Schofstoll, and Brian Fode for their excellent assistance with field experiments. NR 12 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD OCT 30 PY 2016 VL 89 BP 465 EP 467 DI 10.1016/j.indcrop.2016.05.045 PG 3 WC Agricultural Engineering; Agronomy SC Agriculture GA DT1GZ UT WOS:000381231000058 ER PT J AU Day, K Waite, LL Alonso, A Irvin, MR Zhi, D Thibeault, KS Aslibekyan, S Hidalgo, B Borecki, IB Ordovas, JM Arnett, DK Tiwari, HK Absher, DM AF Day, Kenneth Waite, Lindsay L. Alonso, Arnald Irvin, Marguerite R. Zhi, Degui Thibeault, Krista S. Aslibekyan, Stella Hidalgo, Bertha Borecki, Ingrid B. Ordovas, Jose M. Arnett, Donna K. Tiwari, Hemant K. Absher, Devin M. TI Heritable DNA Methylation in CD4(+) Cells among Complex Families Displays Genetic and Non-Genetic Effects SO PLOS ONE LA English DT Article ID TRANSGENERATIONAL EPIGENETIC INHERITANCE; ALLELE-SPECIFIC METHYLATION; EPIGENOME-WIDE ASSOCIATION; QUANTITATIVE TRAIT LOCUS; LIPID-LOWERING DRUGS; HUMAN LIFE-COURSE; DIET NETWORK; FENOFIBRATE TREATMENT; GERMLINE EPIGENOME; MULTIPLE TISSUES AB DNA methylation at CpG sites is both heritable and influenced by environment, but the relative contributions of each to DNA methylation levels are unclear. We conducted a heritability analysis of CpG methylation in human CD4+ cells across 975 individuals from 163 families in the Genetics of Lipid-lowering Drugs and Diet Network (GOLDN). Based on a broad-sense heritability (H-2) value threshold of 0.4, we identified 20,575 highly heritable CpGs among the 174,445 most variable autosomal CpGs (SD > 0.02). Tests for associations of heritable CpGs with genotype at 2,145,360 SNPs using 717 of 975 individuals showed that similar to 74% were cis-meQTLs (< 1 Mb away from the CpG), 6% of CpGs exhibited trans-meQTL associations (> 1 Mb away from the CpG or located on a different chromosome), and 20% of CpGs showed no strong significant associations with genotype (based on a p-value threshold of 1e-7). Genes proximal to the genotype independent heritable CpGs were enriched for functional terms related to regulation of T cell activation. These CpGs were also among those that distinguished T cells from other blood cell lineages. Compared to genes proximal to meQTL-associated heritable CpGs, genotype independent heritable CpGs were moderately enriched in the same genomic regions that escape erasure during primordial germ cell development and could carry potential for generational transmission. C1 [Day, Kenneth; Waite, Lindsay L.; Alonso, Arnald; Thibeault, Krista S.; Absher, Devin M.] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [Waite, Lindsay L.; Zhi, Degui; Hidalgo, Bertha; Tiwari, Hemant K.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Biostat, Birmingham, AL 35294 USA. [Irvin, Marguerite R.; Aslibekyan, Stella; Arnett, Donna K.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. [Borecki, Ingrid B.] Washington Univ, Div Stat Genom, St Louis, MO USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] IMDEA Food, Madrid, Spain. [Alonso, Arnald] Vall dHebron Hosp Res Inst, Rheumatol Res Grp, Barcelona, Spain. [Arnett, Donna K.] Univ Kentucky, Coll Publ Hlth, Lexington, KY USA. RP Absher, DM (reprint author), HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. EM dabsher@hudsonalpha.org FU National Institutes of Health National Heart, Blood and Lung Institute [R01 HL104135-01]; U.S. Public Health Service Resource Grant from the National Center for Research Resources [RR03655] FX This work was supported by the National Institutes of Health National Heart, Blood and Lung Institute (R01 HL104135-01: http://www.nhbi.nih.gov) to DMA and DKA. Some of the results in this paper were obtained by using the software package S.A.G.E., which was supported by a U.S. Public Health Service Resource Grant (RR03655) from the National Center for Research Resources. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 75 TC 0 Z9 0 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 28 PY 2016 VL 11 IS 10 DI 10.1371/journal.pone.0165488 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE3YA UT WOS:000389537000024 ER PT J AU Schicker, RS Rossow, J Eckel, S Fisher, N Bidol, S Tatham, L Matthews-Greer, J Sohner, K Bowman, AS Avrill, J Forshey, T Blanton, L Davis, CT Schiltz, J Skorupski, S Berman, L Jang, YH Bresee, JS Lindstrom, S Trock, SC Wentworth, D Fry, AM de Fijter, S Signs, K DiOrio, M Olsen, SJ Biggerstaff, M AF Schicker, Rebekah S. Rossow, John Eckel, Seth Fisher, Nicolas Bidol, Sally Tatham, Lilith Matthews-Greer, Janice Sohner, Kevin Bowman, Andrew S. Avrill, James Forshey, Tony Blanton, Lenee Davis, C. Todd Schiltz, John Skorupski, Susan Berman, LaShondra Jang, Yunho Bresee, Joseph S. Lindstrom, Stephen Trock, Susan C. Wentworth, David Fry, Alicia M. de Fijter, Sietske Signs, Kimberly DiOrio, Mary Olsen, Sonja J. Biggerstaff, Matthew TI Outbreak of Influenza A(H3N2) Variant Virus Infections Among Persons Attending Agricultural Fairs Housing Infected Swine - Michigan and Ohio, July-August 2016 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID UNITED-STATES; PIGS; H3N2 C1 [Schicker, Rebekah S.] CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA. [Schicker, Rebekah S.; Rossow, John; Blanton, Lenee; Davis, C. Todd; Berman, LaShondra; Jang, Yunho; Bresee, Joseph S.; Lindstrom, Stephen; Trock, Susan C.; Wentworth, David; Fry, Alicia M.; Olsen, Sonja J.; Biggerstaff, Matthew] CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. [Rossow, John] CDC, Epidemiol Elective Program, Div Sci Educ & Profess Dev, Ctr Surveillance Epidemiol & Lab Serv, Atlanta, GA 30333 USA. [Eckel, Seth; Bidol, Sally; Matthews-Greer, Janice; Signs, Kimberly] Michigan Dept Hlth & Human Serv, Lansing, MI USA. [Fisher, Nicolas; Tatham, Lilith; Sohner, Kevin; de Fijter, Sietske; DiOrio, Mary] Ohio Dept Hlth, Columbus, OH 43266 USA. [Bowman, Andrew S.] Ohio State Univ, Coll Vet Med, Columbus, OH 43210 USA. [Avrill, James] Michigan Dept Agr & Rural Dev, Anim Ind Div, Lansing, MI USA. [Forshey, Tony] Ohio Dept Agr, Div Anim Hlth, Reynoldsburg, OH USA. [Schiltz, John] USDA, Natl Vet Serv Labs, Washington, DC 20250 USA. [Skorupski, Susan] USDA, Anim & Plant Hlth Inspect Serv, Washington, DC 20250 USA. RP Schicker, RS (reprint author), CDC, Epidem Intelligence Serv, Atlanta, GA 30333 USA.; Schicker, RS (reprint author), CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. EM rschicker@cdc.gov NR 9 TC 1 Z9 1 U1 0 U2 0 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD OCT 28 PY 2016 VL 65 IS 42 BP 1157 EP 1160 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EA5EZ UT WOS:000386643500001 PM 27787493 ER PT J AU Arbizu, CI Ellison, SL Senalik, D Simon, PW Spooner, DM AF Arbizu, Carlos I. Ellison, Shelby L. Senalik, Douglas Simon, Philipp W. Spooner, David M. TI Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae) SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Carrot; Daucus carota; Genotyping-by-sequencing (GBS); Phylogeny; Single nucleotide polymorphisms (SNPs) ID FRAGMENT-LENGTH-POLYMORPHISMS; GUTTATUS COMPLEX APIACEAE; MISSING DATA; POPULATION-STRUCTURE; GENETIC-STRUCTURE; PHYLOGENETIC ANALYSIS; MITOCHONDRIAL GENOMES; INFERRING PHYLOGENY; MOLECULAR PHYLOGENY; L. GERMPLASM AB Background: The majority of the subspecies of Daucus carota have not yet been discriminated clearly by various molecular or morphological methods and hence their phylogeny and classification remains unresolved. Recent studies using 94 nuclear orthologs and morphological characters, and studies employing other molecular approaches were unable to distinguish clearly many of the subspecies. Fertile intercrosses among traditionally recognized subspecies are well documented. We here explore the utility of single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing (GBS) to serve as an effective molecular method to discriminate the subspecies of the D. carota complex. Results: We used GBS to obtain SNPs covering all nine Daucus carota chromosomes from 162 accessions of Daucus and two related genera. To study Daucus phylogeny, we scored a total of 10,814 or 38,920 SNPs with a maximum of 10 or 30 % missing data, respectively. To investigate the subspecies of D. carota, we employed two data sets including 150 accessions: (i) rate of missing data 10 % with a total of 18,565 SNPs, and (ii) rate of missing data 30 %, totaling 43,713 SNPs. Consistent with prior results, the topology of both data sets separated species with 2n = 18 chromosome from all other species. Our results place all cultivated carrots (D. carota subsp. sativus) in a single clade. The wild members of D. carota from central Asia were on a clade with eastern members of subsp. sativus. The other subspecies of D. carota were in four clades associated with geographic groups: (1) the Balkan Peninsula and the Middle East, (2) North America and Europe, (3) North Africa exclusive of Morocco, and (4) the Iberian Peninsula and Morocco. Daucus carota subsp. maximus was discriminated, but neither it, nor subsp. gummifer (defined in a broad sense) are monophyletic. Conclusions: Our study suggests that (1) the morphotypes identified as D. carota subspecies gummifer (as currently broadly circumscribed), all confined to areas near the Atlantic Ocean and the western Mediterranean Sea, have separate origins from sympatric members of other subspecies of D. carota, (2) D. carota subsp. maximus, on two clades with some accessions of subsp. carota, can be distinguished from each other but only with poor morphological support, (3) D. carota subsp. capillifolius, well distinguished morphologically, is an apospecies relative to North African populations of D. carota subsp. carota, (4) the eastern cultivated carrots have origins closer to wild carrots from central Asia than to western cultivated carrots, and (5) large SNP data sets are suitable for species-level phylogenetic studies in Daucus. C1 [Arbizu, Carlos I.; Ellison, Shelby L.; Senalik, Douglas; Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Senalik, Douglas; Simon, Philipp W.; Spooner, David M.] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. RP Spooner, DM (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Spooner, DM (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov FU USDA Agricultural Research Service; National Council of Science and Technology of Peru (Concytec) FX This work is supported by the USDA Agricultural Research Service. C. Arbizu is partly funded by the National Council of Science and Technology of Peru (Concytec). NR 127 TC 0 Z9 0 U1 13 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD OCT 28 PY 2016 VL 16 AR 234 DI 10.1186/s12862-016-0806-x PG 16 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA EA0XS UT WOS:000386313500002 PM 27793080 ER PT J AU Brocca, L Pellarin, T Crow, WT Ciabatta, L Massari, C Ryu, D Su, CH Rudiger, C Kerr, Y AF Brocca, Luca Pellarin, Thierry Crow, Wade T. Ciabatta, Luca Massari, Christian Ryu, Dongryeol Su, Chun-Hsu Rudiger, Christoph Kerr, Yann TI Rainfall estimation by inverting SMOS soil moisture estimates: A comparison of different methods over Australia SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE soil moisture; rainfall; remote sensing; SMOS ID TIME SATELLITE PRECIPITATION; SOUTHEAST AUSTRALIA; ANALYSIS TMPA; LAND-SURFACE; DATA-SETS; AMSR-E; SCALE; ASSIMILATION; RETRIEVALS; PRODUCTS AB Remote sensing of soil moisture has reached a level of maturity and accuracy for which the retrieved products can be used to improve hydrological and meteorological applications. In this study, the soil moisture product from the Soil Moisture and Ocean Salinity (SMOS) satellite is used for improving satellite rainfall estimates obtained from the Tropical Rainfall Measuring Mission multisatellite precipitation analysis product (TMPA) using three different bottom up techniques: SM2RAIN, Soil Moisture Analysis Rainfall Tool, and Antecedent Precipitation Index Modification. The implementation of these techniques aims at improving the well-known top down rainfall estimate derived from TMPA products (version 7) available in near real time. Ground observations provided by the Australian Water Availability Project are considered as a separate validation data set. The three algorithms are calibrated against the gauge-corrected TMPA reanalysis product, 3B42, and used for adjusting the TMPA real-time product, 3B42RT, using SMOS soil moisture data. The study area covers the entire Australian continent, and the analysis period ranges from January 2010 to November 2013. Results show that all the SMOS-based rainfall products improve the performance of 3B42RT, even at daily time scale (differently from previous investigations). The major improvements are obtained in terms of estimation of accumulated rainfall with a reduction of the root-mean-square error of more than 25%. Also, in terms of temporal dynamic (correlation) and rainfall detection (categorical scores) the SMOS-based products provide slightly better results with respect to 3B42RT, even though the relative performance between the methods is not always the same. The strengths and weaknesses of each algorithm and the spatial variability of their performances are identified in order to indicate the ways forward for this promising research activity. Results show that the integration of bottom up and top down approaches has the potential to improve the quality of near-real-time rainfall estimates from remote sensing in the near future. C1 [Brocca, Luca; Ciabatta, Luca; Massari, Christian] CNR, Res Inst Geohydrol Protect, Perugia, Italy. [Pellarin, Thierry] Univ Grenoble Alpes, CNRS, LTHE, Grenoble, France. [Crow, Wade T.] Hydrol & Remote Sensing Lab, USDA ARS, Beltsville, MD USA. [Ryu, Dongryeol; Su, Chun-Hsu] Univ Melbourne, Dept Infrastruct Engn, Melbourne, Vic, Australia. [Rudiger, Christoph] Monash Univ, Dept Civil Engn, Clayton, Vic, Australia. [Kerr, Yann] CNRS, CNES, IRD, Ctr Etudes Spati Biosphere UPS, Toulouse, France. RP Brocca, L (reprint author), CNR, Res Inst Geohydrol Protect, Perugia, Italy. EM luca.brocca@irpi.cnr.it RI Brocca, Luca/F-2854-2010 OI Brocca, Luca/0000-0002-9080-260X FU European Space Agency SMOS+rainfall project [4000114738/15/I-SBo]; Centre National d'Etudes Spatiales TOSCA FX We would like to acknowledge the Australian Bureau of Meteorology for providing daily rainfall data (accessed online via http://www.bom.gov.au/jsp/awap/rain/archive_recal.jsp), the support of the European Space Agency SMOS+rainfall project (contract 4000114738/15/I-SBo), and of the Centre National d'Etudes Spatiales TOSCA. SMOS L3SM product was provided by the Centre Aval de Traitement des Donnees SMOS (CATDS), developed by CNES in collaboration with CESBIO and IFREMER. All the input data sets used in the paper are available for free through public websites. The four rainfall products estimated/corrected by using SMOS soil moisture data are available upon request from the corresponding author. NR 56 TC 1 Z9 1 U1 8 U2 8 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD OCT 27 PY 2016 VL 121 IS 20 BP 12062 EP 12079 DI 10.1002/2016JD025382 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA EC7CJ UT WOS:000388293100034 ER PT J AU Spackman, E Cardona, C Munoz-Aguayo, J Fleming, S AF Spackman, Erica Cardona, Carol Munoz-Aguayo, Jeannette Fleming, Susan TI Successes and Short Comings in Four Years of an International External Quality Assurance Program for Animal Influenza Surveillance SO PLoS One LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; VIRUS; PROFICIENCY; ANTIBODIES; DIAGNOSIS AB The US National institutes of Health-Centers of Excellence for Influenza Research and Surveillance is a research consortium that funds numerous labs worldwide to conduct influenza A surveillance in diverse animal species. There is no harmonization of testing procedures among these labs; therefore an external quality assurance (EQA) program was implemented to evaluate testing accuracy among labs in the program in 2012. Accurate detection of novel influenza A variants is crucial because of the broad host range and potentially high virulence of the virus in diverse species. Two molecular detection sample sets and 2 serology sample sets (one with avian origin isolates, and one with mammalian origin isolates each) were made available at approximately six month intervals. Participating labs tested the material in accordance with their own protocols. During a five year period a total of 41 labs from 23 countries ordered a total of 132 avian molecular, 121 mammalian molecular and 90 serology sample sets. Testing was completed by 111 individuals. Detection of type A influenza by RT-PCR was reliable with a pass rate (80% or greater agreement with expected results) of 86.6% for avian and 86.2% for mammalian origin isolates. However, identification of subtype by RT-PCR was relatively poor with 54.1% and 75.9% accuracy for avian and mammalian influenza isolates respectively. Serological testing had an overall pass rate of 86.9% and 22/23 labs used commercial ELISA kits. Based on the results of this EQA program six labs modified their procedures to improve accuracy and one lab identified an unknown equipment problem. These data represent the successful implementation of an international EQA program for an infectious disease; insights into the logistics and test design are also discussed. C1 [Spackman, Erica] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. [Cardona, Carol; Munoz-Aguayo, Jeannette; Fleming, Susan] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. RP Spackman, E (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM Erica.Spackman@ars.usda.gov FU US Department of Agriculture, ARS CRIS Project [6040-32000-063-00D]; National Institute of Allergy and Infectious Diseases, National Institutes of Health [AAI12004-001-00001]; Minnesota Center for Excellence in Influenza Research and Surveillance [HHSN266200700007] FX Support was provided by US Department of Agriculture, ARS CRIS Project 6040-32000-063-00D and by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA No. AAI12004-001-00001 and the Minnesota Center for Excellence in Influenza Research and Surveillance HHSN266200700007. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; Support was provided by US Department of Agriculture, ARS CRIS Project 6040-32000063-00D and by federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, under IAA No. AAI12004-001-00001 and the Minnesota Center for Excellence in Influenza Research and Surveillance HHSN266200700007. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 16 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 27 PY 2016 VL 11 IS 10 AR e0164261 DI 10.1371/journal.pone.0164261 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE4VY UT WOS:000389604900015 PM 27788155 ER PT J AU Paneru, B Al-Tobasei, R Palti, Y Wiens, GD Salem, M AF Paneru, Bam Al-Tobasei, Rafet Palti, Yniv Wiens, Gregory D. Salem, Mohamed TI Differential expression of long non-coding RNAs in three genetic lines of rainbow trout in response to infection with Flavobacterium psychrophilum SO SCIENTIFIC REPORTS LA English DT Article ID NEUTROPHIL ELASTASE; ONCORHYNCHUS-MYKISS; INNATE IMMUNITY; UP-REGULATION; CUTTING EDGE; IN-VIVO; ACTIVATION; CELLS; PATHWAY; PROTEIN AB Bacterial cold-water disease caused by Flavobacterium psychrophilum is one of the major causes of mortality of salmonids. Three genetic lines of rainbow trout designated as ARS-Fp-R (resistant), ARS-Fp-C (control) and ARS-Fp-S (susceptible) have significant differences in survival rate following F. psychrophilum infection. Previous study identified transcriptome differences of immune-relevant protein-coding genes at basal and post infection levels among these genetic lines. Using RNA-Seq approach, we quantified differentially expressed (DE) long non-coding RNAs (lncRNAs) in response to F. psychrophilum challenge in these genetic lines. Pairwise comparison between genetic lines and different infection statuses identified 556 DE lncRNAs. A positive correlation existed between the number of the differentially regulated lncRNAs and that of the protein-coding genes. Several lncRNAs showed strong positive and negative expression correlation with their overlapped, neighboring and distant immune related protein-coding genes including complement components, cytokines, chemokines and several signaling molecules involved in immunity. The correlated expressions and genome-wide co-localization suggested that some lncRNAs may be involved in regulating immune-relevant protein-coding genes. This study provides the first evidence of lncRNA-mediated regulation of the anti-bacterial immune response in a commercially important aquaculture species and will likely help developing new genetic markers for rainbow trout disease resistance. C1 [Paneru, Bam; Salem, Mohamed] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. [Paneru, Bam; Salem, Mohamed] Middle Tennessee State Univ, Mol Biosci Program, Murfreesboro, TN 37132 USA. [Al-Tobasei, Rafet; Salem, Mohamed] Middle Tennessee State Univ, Computat Sci Program, Murfreesboro, TN 37132 USA. [Palti, Yniv; Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Salem, M (reprint author), Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA.; Salem, M (reprint author), Middle Tennessee State Univ, Mol Biosci Program, Murfreesboro, TN 37132 USA.; Salem, M (reprint author), Middle Tennessee State Univ, Computat Sci Program, Murfreesboro, TN 37132 USA. EM Mohamed.salem@mtsu.edu FU Agricultural Research Service CRIS Project "Integrated Approaches for Improving Aquatic Animal Health in Cool and Cold Water Aquaculture" [1930-32000-005]; CRIS Project "Utilization of Genomics for Improving Production Traits in Cool and Cold Water Aquaculture" [1930-32000-009]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2012-67015-30217, 2014-67015-21602] FX We acknowledge technical contributions from Travis Moreland and Joel Caren. This work was supported by Agricultural Research Service CRIS Project 1930-32000-005 "Integrated Approaches for Improving Aquatic Animal Health in Cool and Cold Water Aquaculture," CRIS Project 1930-32000-009 "Utilization of Genomics for Improving Production Traits in Cool and Cold Water Aquaculture" and Agriculture and Food Research Initiative competitive grants no. 2012-67015-30217 (to Gregory D. Wiens) and No. 2014-67015-21602 (to Mohamed Salem) from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US, Department of Agriculture. USDA is an equal opportunity employer. NR 66 TC 2 Z9 2 U1 7 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 27 PY 2016 VL 6 AR 36032 DI 10.1038/srep36032 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA0XK UT WOS:000386312000001 PM 27786264 ER PT J AU Lerch, AP Pfammatter, JA Bentz, BJ Raffa, KF AF Lerch, Andrew P. Pfammatter, Jesse A. Bentz, Barbara J. Raffa, Kenneth F. TI Mountain Pine Beetle Dynamics and Reproductive Success in Post-Fire Lodgepole and Ponderosa Pine Forests in Northeastern Utah SO PLOS ONE LA English DT Article ID FIRE SURROGATE TREATMENTS; BARK BEETLE; TREE MORTALITY; PRESCRIBED FIRE; SPATIOTEMPORAL PATTERNS; NORTHERN ARIZONA; ROCKY-MOUNTAINS; RESIN DEFENSES; ATTACKS; OUTBREAKS AB Fire injury can increase tree susceptibility to some bark beetles (Curculionidae, Scolytinae), but whether wildfires can trigger outbreaks of species such as mountain pine beetle (Den-droctonus ponderosae Hopkins) is not well understood. We monitored 1173 lodgepole (Pinus contorta var. latifolia Doug.) and 599 ponderosa (Pinus ponderosa Doug. ex Law) pines for three years post-wildfire in the Uinta Mountains of northeastern Utah in an area with locally endemic mountain pine beetle. We examined how the degree and type of fire injury influenced beetle attacks, brood production, and subsequent tree mortality, and related these to beetle population changes over time. Mountain pine beetle population levels were high the first two post-fire years in lodgepole pine, and then declined. In ponderosa pine, populations declined each year after initial post-fire sampling. Compared to trees with strip or failed attacks, mass attacks occurred on trees with greater fire injury, in both species. Overall, a higher degree of damage to crowns and boles was associated with higher attack rates in ponderosa pines, but additional injury was more likely to decrease attack rates in lodgepole pines. In lodgepole pine, attacks were initially concentrated on fireinjured trees, but during subsequent years beetles attacked substantial numbers of uninjured trees. In ponderosa pine, attacks were primarily on injured trees each year, although these stands were more heavily burned and had few uninjured trees. In total, 46% of all lodgepole and 56% of ponderosa pines underwent some degree of attack. Adult brood emergence within caged bole sections decreased with increasing bole char in lodgepole pine but increased in ponderosa pine, however these relationships did not scale to whole trees. Mountain pine beetle populations in both tree species four years post-fire were substantially lower than the year after fire, and wildfire did not result in population outbreaks. C1 [Lerch, Andrew P.; Raffa, Kenneth F.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Pfammatter, Jesse A.] Univ Wisconsin, Dept Neurosci, Madison, WI USA. [Bentz, Barbara J.] USDA, Forest Serv, Rocky Mt Res Stn, Logan, UT USA. RP Lerch, AP (reprint author), Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. EM lerch.andrew@gmail.com FU USDA Forest Service, State and Private Forestry, Forest Health Monitoring [INT-F-08-01] FX USDA Forest Service, State and Private Forestry, Forest Health Monitoring, INT-F-08-01; BJB KFR. NR 50 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 26 PY 2016 VL 11 IS 10 AR e0164738 DI 10.1371/journal.pone.0164738 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EE4VE UT WOS:000389602800047 PM 27783632 ER PT J AU Ravi, S Sharratt, BS Li, JR Olshevski, S Meng, ZJ Zhang, JG AF Ravi, Sujith Sharratt, Brenton S. Li, Junran Olshevski, Stuart Meng, Zhongju Zhang, Jianguo TI Particulate matter emissions from biochar-amended soils as a potential tradeoff to the negative emission potential SO SCIENTIFIC REPORTS LA English DT Article ID THRESHOLD FRICTION VELOCITY; CLIMATE-CHANGE; CARBON; REMOVAL; PARTICLES; BIOFILTER; BLACK; WIND AB Novel carbon sequestration strategies such as large-scale land application of biochar may provide sustainable pathways to increase the terrestrial storage of carbon. Biochar has a long residence time in the soil and hence comprehensive studies are urgently needed to quantify the environmental impacts of large-scale biochar application. In particular, black carbon emissions from soils amended with biochar may counteract the negative emission potential due to the impacts on air quality, climate, and biogeochemical cycles. We investigated, using wind tunnel experiments, the particulate matter emission potential of a sand and two agriculturally important soils amended with different concentrations of biochar, in comparison to control soils. Our results indicate that biochar application considerably increases particulate emissions possibly by two mechanisms-the accelerated emission of fine biochar particles and the generation and emission of fine biochar particles resulting from abrasion of large biochar particles by sand grains. Our study highlights the importance of considering the background soil properties (e.g., texture) and geomorphological processes (e.g., aeolian transport) for biochar-based carbon sequestration programs. C1 [Ravi, Sujith; Olshevski, Stuart] Temple Univ, Dept Earth & Environm Sci, Philadelphia, PA 19122 USA. [Sharratt, Brenton S.; Meng, Zhongju; Zhang, Jianguo] USDA ARS, Northwest Sustainable Agroecosyst Res, Pullman, WA 99164 USA. [Li, Junran] Univ Tulsa, Dept Geosci, Tulsa, OK 74104 USA. [Meng, Zhongju] Inner Mongolia Agr Univ, Coll Desert Sci & Engn, Hohhot 010019, Inner Mongolia, Peoples R China. [Zhang, Jianguo] Northwest A&F Univ, Coll Nat Resources & Environm, Xianyang 712100, Shaanxi, Peoples R China. RP Ravi, S (reprint author), Temple Univ, Dept Earth & Environm Sci, Philadelphia, PA 19122 USA. EM sravi@temple.edu FU US National Science Foundation [EAR-1451518] FX S.R. and J.L. acknowledge partial support from the US National Science Foundation (EAR-1451518). *Identification of experimental apparatus or company sources is for information purposes only and does not imply endorsement by ARS, USDA. NR 34 TC 0 Z9 0 U1 14 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 26 PY 2016 VL 6 AR 35984 DI 10.1038/srep35984 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ8HS UT WOS:000386112100001 PM 27782159 ER PT J AU Gilligan, T Huemer, P Wiesmair, B AF Gilligan, Todd Huemer, Peter Wiesmair, Benjamin TI Different continents, same species? Resolving the taxonomy of some Holarctic Ancylis Hubner (Lepidoptera: Tortricidae) SO ZOOTAXA LA English DT Article DE christiandiana; DNA barcoding; Enarmoniini; oregonensis; saliana ID GENITALIA; EVOLUTION AB Several species of Ancylis related to A. unguicella (Linnaeus) and A. geminana (Donovan) have been presumed by previous authors to be Holarctic. However, difficulty in identifying genitalic characters to define and separate these taxa has brought into question their true distribution and led to inconsistencies in their taxonomic treatment in Europe and North America. Here we use a combination of DNA barcode sequence data and morphology to resolve these taxonomic differences, determine the actual geographic range of these taxa, and describe three new species. In the A. unguicella group, only A. unguicella and A. uncella (Denis & Schiffermuller) are Holarctic in distribution. In the A. geminana group, none of the taxa are Holarctic in their distribution. Three species are described as new: A. christiandiana Huemer and Wiesmair, sp.n. (Austria, Germany); A. oregonensis Gilligan and Huemer, sp.n. (USA: Oregon); and A. saliana Gilligan and Huemer, sp.n. (USA: Florida). In addition, Ancylis carbonana Heinrich, syn.n., is synonymized with A. uncella; A. cuspidana (Treitschke), syn.rev., is synonymized with A. diminutana (Haworth); and A. diminuatana Kearfott, stat.rev., and A. subarcuana (Douglas), stat.rev., are raised from synonymy. C1 [Gilligan, Todd] APHIS, Identificat Technol Program, USDA, PPQ,S&T, 2301 Res Blvd,Suite 108, Ft Collins, CO 80526 USA. [Huemer, Peter; Wiesmair, Benjamin] Tiroler Landesmuseen Betriebgsges mbH, Nat Wissensch Sammlungen, Feldstr 11a, A-6020 Innsbruck, Austria. RP Gilligan, T (reprint author), APHIS, Identificat Technol Program, USDA, PPQ,S&T, 2301 Res Blvd,Suite 108, Ft Collins, CO 80526 USA. EM todd.m.gilligan@aphis.usda.gov; p.huemer@tiroler-landesmuseen.at; b.wiesmair@tiroler-landesmuseen.at FU Government of Canada; Ontario Ministry of Research and Innovation; NSERC; Promotion of Educational Policies, University and Research Department of the Autonomous Province of Bolzano-South Tyrol; Austrian Federal Ministry of Science, Research and Economics FX We are particularly grateful to Paul Hebert and his team at the Canadian Centre for DNA Barcoding (Guelph, Canada) who's sequencing work was enabled by funding from the Government of Canada to Genome Canada through the Ontario Genomics Institute. We are also grateful to the Ontario Ministry of Research and Innovation and to NSERC for their support of the BOLD informatics platform. PH is furthermore indebted to the Promotion of Educational Policies, University and Research Department of the Autonomous Province of Bolzano-South Tyrol for helping to fund the project "Genetic biodiversity archive-DNA barcoding of Lepidoptera of the central Alpine region (South, East and North Tyrol)", and to the Austrian Federal Ministry of Science, Research and Economics for funds received in the framework of ABOL (Austrian Barcode of Life). NR 47 TC 0 Z9 0 U1 2 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD OCT 26 PY 2016 VL 4178 IS 3 BP 347 EP 370 DI 10.11646/zootaxa.4178.3.3 PG 24 WC Zoology SC Zoology GA DZ6YE UT WOS:000386007900003 PM 27811713 ER PT J AU Clarke, CR Hayes, BW Runde, BJ Markel, E Swingle, BM Vinatzer, BA AF Clarke, Christopher R. Hayes, Byron W. Runde, Brendan J. Markel, Eric Swingle, Bryan M. Vinatzer, Boris A. TI Comparative genomics of Pseudomonas syringae pathovar tomato reveals novel chemotaxis pathways associated with motility and plant pathogenicity SO PEERJ LA English DT Article DE Chemotaxis; Flagellin; Swimming motility; CheA; Swarming motility; Twitching motility; DC3000; Pto ID PV. TABACI 6605; TWITCHING MOTILITY; IV PILI; BIOFILM FORMATION; RHODOBACTER-SPHAEROIDES; BACTERIAL CHEMOTAXIS; SIGNAL-TRANSDUCTION; ROOT COLONIZATION; GENE-CLUSTER; CHEY GENES AB The majority of bacterial foliar plant pathogens must invade the apoplast of host plants through points of ingress, such as stomata or wounds, to replicate to high population density and cause disease. How pathogens navigate plant surfaces to locate invasion sites remains poorly understood. Many bacteria use chemical-directed regulation of flagellar rotation, a process known as chernotaxis, to move towards favorable environmental conditions. Chernotactic sensing of the plant surface is a potential mechanism through which foliar plant pathogens home in on wounds or stomata, but chernotactic systems in foliar plant pathogens are not well characterized. Comparative genomics of the plant pathogen Pseudomonas syringae pathovar tomato (Pto) implicated annotated chemotwds genes in the recent adaptations of one Pto lineage. We therefore characterized the chemosensory system of Pto. The Pto genome contains two primary chemotaxis gene clusters, che1 and che2. The che2 cluster is flanked by flagellar biosynthesis genes and similar to the canonical chemotwds gene clusters of other bacteria based on sequence and synteny. Disruption of the primary phosphorelay kinase gene of the che2 cluster, cheA2, eliminated all swimming and surface motility at 21 degrees C but not 28 degrees C for Pto. The chel cluster is located next to Type IV pili biosynthesis genes but disruption of cheA1 has no observable effect on twitching motility for Pto. Disruption of cheA2 also alters in planta fitness of the pathogen with strains lacking functional cheA2 being less fit in host plants but more fit in a non-host interaction. C1 [Clarke, Christopher R.; Hayes, Byron W.; Runde, Brendan J.; Vinatzer, Boris A.] Virginia Tech, Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Markel, Eric; Swingle, Bryan M.] USDA, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. [Swingle, Bryan M.] Sch Integrat Plant, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY USA. RP Clarke, CR (reprint author), Virginia Tech, Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM gtg681r@vt.edu FU NSF [IOS-0746501, IOS-1354215]; Virginia Agricultural Experiment Station; Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture; National Institute of Food and Agriculture postdoctoral research fellowship [2015-67012-22821] FX This work was supported by NSF IOS-0746501 and IOS-1354215 to BAV. Funding to BAV was also provided in part by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, US Department of Agriculture. CRC is supported by a National Institute of Food and Agriculture postdoctoral research fellowship (2015-67012-22821). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 10 U2 10 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD OCT 25 PY 2016 VL 4 AR e2570 DI 10.7717/peerj.2570 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EA2AB UT WOS:000386392300002 PM 27812402 ER PT J AU Ramey, AM Spackman, E Kim-Torchetti, M DeLiberto, TJ AF Ramey, Andrew M. Spackman, Erica Kim-Torchetti, Mia DeLiberto, Thomas J. TI Weak support for disappearance and restricted emergence/persistence of highly pathogenic influenza A in North American waterfowl SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter ID WILD BIRDS; SOUTH-KOREA; VIRUSES; H5N8 C1 [Ramey, Andrew M.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Spackman, Erica] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. [Kim-Torchetti, Mia] Vet Serv, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, USDA, Ames, IA 50010 USA. [DeLiberto, Thomas J.] Wildlife Serv, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80521 USA. RP Ramey, AM (reprint author), US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. EM aramey@usgs.gov NR 10 TC 1 Z9 1 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 25 PY 2016 VL 113 IS 43 BP E6551 EP E6552 DI 10.1073/pnas.1614530113 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ7ZI UT WOS:000386087100003 PM 27791026 ER PT J AU Kushanov, FN Pepper, AE Yu, JZ Buriev, ZT Shermatov, SE Saha, S Ulloa, M Jenkins, JN Abdukarimov, A Abdurakhmonov, IY AF Kushanov, Fakhriddin N. Pepper, Alan E. Yu, John Z. Buriev, Zabardast T. Shermatov, Shukhrat E. Saha, Sukumar Ulloa, Mauricio Jenkins, Johnie N. Abdukarimov, Abdusattor Abdurakhmonov, Ibrokhim Y. TI Development, genetic mapping and QTL association of cotton PHYA, PHYB, and HY5-specific CAPS and dCAPS markers SO BMC GENETICS LA English DT Article DE Cotton; Phytochromes; CAPS and dCAPS; Linkage mapping; Fiber QTLs ID SINGLE NUCLEOTIDE POLYMORPHISMS; GOSSYPIUM-HIRSUTUM L.; B-BOX PROTEIN; ARABIDOPSIS-THALIANA; TETRAPLOID COTTON; MOLECULAR MARKERS; FIBER QUALITY; PHYTOCHROME B; UPLAND COTTON; EXPRESSION AB Background: Among SNP markers that become increasingly valuable in molecular breeding of crop plants are the CAPS and dCAPS markers derived from the genes of interest. To date, the number of such gene-based markers is small in polyploid crop plants such as allotetraploid cotton that has A-and D-sub-genomes. The objective of this study was to develop and map new CAPS and dCAPS markers for cotton developmental-regulatory genes that are important in plant breeding programs. Results: Gossypium hirsutum and G. barbadense, are the two cultivated allotetraploid cotton species. These have distinct fiber quality and other agronomic traits. Using comparative sequence analysis of characterized GSTs of the PHYA1, PHYB, and HY5 genes of G. hirsutum and G. barbadense one PHYA1-specific Mbo I/Dpn II CAPS, one PHYB-specific Alu I dCAPS, and one HY5-specific Hinf I dCAPS cotton markers were developed. These markers have successfully differentiated the two allotetraploid genomes (AD(1) and AD(2)) when tested in parental genotypes of 'Texas Marker-1' ('TM-1'), 'Pima 3-79' and their F-1 hybrids. The genetic mapping and chromosome substitution line-based deletion analyses revealed that PHYA1 gene is located in A-sub-genome chromosome 11, PHYB gene is in A-sub-genome chromosome 10, and HY5 gene is in D-sub-genome chromosome 24, on the reference 'TM-1' x 'Pima 3-79' RIL genetic map. Further, it was found that genetic linkage map regions containing phytochrome and HY5-specific markers were associated with major fiber quality and flowering time traits in previously published QTL mapping studies. Conclusion: This study detailed the genome mapping of three cotton phytochrome genes with newly developed CAPS and dCAPS markers. The proximity of these loci to fiber quality and other cotton QTL was demonstrated in two A-subgenome and one D-subgenome chromosomes. These candidate gene markers will be valuable for marker-assisted selection (MAS) programs to rapidly introgress G. barbadense phytochromes and/or HY5 gene (s) into G. hirsutum cotton genotypes or vice versa. C1 [Kushanov, Fakhriddin N.; Buriev, Zabardast T.; Shermatov, Shukhrat E.; Abdukarimov, Abdusattor; Abdurakhmonov, Ibrokhim Y.] Acad Sci Uzbek, Ctr Genom & Bioinformat, Univ St 2, Qibray Region 111215, Uzbekistan. [Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Yu, John Z.] USDA ARS, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77843 USA. [Saha, Sukumar; Jenkins, Johnie N.] USDA ARS, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Ulloa, Mauricio] USDA ARS, Plant Stress & Germplasm Dev Res, 3810 4th St, Lubbock, TX 79415 USA. RP Abdurakhmonov, IY (reprint author), Acad Sci Uzbek, Ctr Genom & Bioinformat, Univ St 2, Qibray Region 111215, Uzbekistan. EM genomics@uzsci.net; ibrokhim.abdurakhmonov@genomics.uz FU Office of International Research Programs of the United States Department of Agriculture (USDA) under the USDA-Former Soviet Union [P121/P121B]; Academy of science of Uzbekistan [FA-F5-T030] FX This research was funded by the Office of International Research Programs of the United States Department of Agriculture (USDA) under the USDA-Former Soviet Union cooperation programs with the research Grant of P121/P121B to IYA, SS and AEP. The research is also funded by Academy of science of Uzbekistan with the local research grant of FA-F5-T030. NR 59 TC 0 Z9 0 U1 10 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD OCT 24 PY 2016 VL 17 AR 141 DI 10.1186/s12863-016-0448-4 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DZ6OS UT WOS:000385982400001 PM 27776497 ER PT J AU Tillman, PG Cottrell, TE AF Tillman, P. Glynn Cottrell, Ted E. TI Density and Egg Parasitism of Stink Bugs (Hemiptera:Pentatomidae) in Elderberry and Dispersal Into Crops SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Noncrop host plant; dispersal; cotton; Anastatus; Trissolcus ID HETEROPTERA-PENTATOMIDAE; HEMIPTERA-PENTATOMIDAE; ASHMEAD HYMENOPTERA; EUSCHISTUS-SERVUS; SOUTH-CAROLINA; PECAN ORCHARDS; FIELD EDGES; COTTON; PHEROMONE; AGGREGATION AB Chinavia hilaris (Say), Euschistus servus (Say), Euschistus tristigmus (Say), and Thyanta custator custator (F.) (Hemiptera: Pentatomidae) are serious pests of crops in the southeastern United States but little is known concerning their dispersal from noncrop hosts in woodlands into crops. This 2-yr study was conducted to investigate whether elderberry [Sambucus nigra subsp. canadensis (L.) R. Bolli] in woodlands serves as a source of stink bugs dispersing into adjacent crops and to examine parasitism of C. hilaris and E. servus eggs on this plant. Elderberry was a reproductive host for each of the four stink bug species; females oviposited on plants with subsequent nymphs feeding on elderberry and developing into adults. Anastatus mirabilis (Walsh & Riley) (Hymenoptera: Eupelmidae), Anastatus reduvii (Howard), and Trissolcus edessae Fouts (Hymenoptera: Scelionidae) were prevalent egg parasitoids of C. hilaris but A. reduvii was the prevalent parasitoid of E. servus. Newly developed stink bug adults were first detected on elderberry around mid-July. Then in late July and early August, as elderberry fruit senesced and cotton bolls became available, stink bugs began dispersing from elderberry into cotton based on recapture of stink bugs on cotton that had previously been marked on elderberry. In addition, in 2015, density of C. hilaris, E. servus, and E. tristigmus was higher in cotton with elderberry than in cotton without it. Over the study, economic threshold was reached for four of seven cotton fields. Elimination of elderberry in woodlands adjacent to cotton may be a viable management tactic for control of stink bugs in cotton. C1 [Tillman, P. Glynn] ARS, USDA, Crop Protect & Management Res Lab, 2747 Davis Rd, Tifton, GA 31793 USA. [Cottrell, Ted E.] Agr Res Serv, USDA, Southeastern Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, 2747 Davis Rd, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov; Ted.Cottrell@ars.usda.gov NR 41 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD OCT 23 PY 2016 VL 16 AR 106 DI 10.1093/jisesa/iew091 PG 14 WC Entomology SC Entomology GA EA6EP UT WOS:000386719300002 ER PT J AU Lin, CY Jakes, JE Donohoe, BS Ciesielski, PN Yang, HB Gleber, SC Vogt, S Ding, SY Peer, WA Murphy, AS McCann, MC Himmel, ME Tucker, MP Wei, H AF Lin, Chien-Yuan Jakes, Joseph E. Donohoe, Bryon S. Ciesielski, Peter N. Yang, Haibing Gleber, Sophie-Charlotte Vogt, Stefan Ding, Shi-You Peer, Wendy A. Murphy, Angus S. McCann, Maureen C. Himmel, Michael E. Tucker, Melvin P. Wei, Hui TI Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Ferritin; Iron co-catalyst; Iron accumulation; Transgenic Arabidopsis; Biomass; High-throughput hot-water pretreatment; Saccharification; Sugar release; Perls' Prussian blue staining; X-ray fluorescence microscopy ID RAY-FLUORESCENCE MICROSCOPY; DILUTE-ACID PRETREATMENT; SOYBEAN FERRITIN GENE; HOMEOSTASIS ALTERATION; ARABIDOPSIS MUTANT; LIGNIN COMPOSITION; ESCHERICHIA-COLI; EPITHELIAL-CELLS; BINDING-PROTEIN; CICER-ARIETINUM AB Background: Plant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However, directly adding iron catalysts into biomass prior to pretreatment is diffusion limited, and increases the cost of biorefinery operations. Recently, we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this study, we extend this approach by fusing the heterologous ferritin gene with a signal peptide for secretion into Arabidopsis cell walls (referred to here as FerEX). Results: The transgenic Arabidopsis plants. FerEX. accumulated iron under both normal and iron-fertilized growth conditions; under the latter (iron-fertilized) condition, FerEX transgenic plants showed an increase in plant height and dry weight by 12 and 18 %, respectively, compared with the empty vector control plants. The SDS- and native-PAGE separation of cell-wall protein extracts followed by Western blot analyses confirmed the extracellular expression of ferritin in FerEX plants. Meanwhile, Perls' Prussian blue staining and X-ray fluorescence microscopy (XFM) maps revealed iron depositions in both the secondary and compound middle lamellae cell-wall layers, as well as in some of the corner compound middle lamella in FerEX. Remarkably, their harvested biomasses showed enhanced pretreatability and digestibility, releasing, respectively, 21 % more glucose and 34 % more xylose than the empty vector control plants. These values are significantly higher than those of our recently obtained ferritin intracellularly expressed plants. Conclusions: This study demonstrated that extracellular expression of ferritin in Arabidopsis can produce plants with increased growth and iron accumulation, and reduced thermal and enzymatic recalcitrance. The results are attributed to the intimate colocation of the iron co-catalyst and the cellulose and hemicellulose within the plant cell-wall region, supporting the genetic modification strategy for incorporating conversion catalysts into energy crops prior to harvesting or processing at the biorefinery. C1 [Lin, Chien-Yuan; Donohoe, Bryon S.; Ciesielski, Peter N.; Ding, Shi-You; Himmel, Michael E.; Wei, Hui] Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA. [Jakes, Joseph E.] US Forest Serv, Forest Biopolymer Sci & Engn, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Yang, Haibing; McCann, Maureen C.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Gleber, Sophie-Charlotte; Vogt, Stefan] Argonne Natl Lab, Adv Photon Source, Xray Sci Div, Argonne, IL 60439 USA. [Peer, Wendy A.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Murphy, Angus S.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Tucker, Melvin P.] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA. [Ding, Shi-You] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. RP Wei, H (reprint author), Natl Renewable Energy Lab, Biosci Ctr, Golden, CO 80401 USA.; Tucker, MP (reprint author), Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA. EM Melvin.Tucker@nrel.gov; Hui.Wei@nrel.gov FU Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio); Energy Frontier Research Center - US Department of Energy, the Office of Science, at the Office of Basic Energy Sciences [DE-SC0000997]; US Department of Energy [DE-AC36-08-GO28308]; US Department of Energy, the Office of Science, at Office of Basic Energy Sciences [W-31-109-Eng-38]; USDA PECASE award FX This work was supported by the Center for Direct Catalytic Conversion of Biomass to Biofuels (C3Bio), an Energy Frontier Research Center funded by the US Department of Energy, the Office of Science, at the Office of Basic Energy Sciences under Award Number DE-SC0000997. The National Renewable Energy Laboratory (NREL) is operated for the US Department of Energy under Contract No. DE-AC36-08-GO28308. The use of Advanced Photon Source facilities was supported by the US Department of Energy, the Office of Science, at Office of Basic Energy Sciences under contract number W-31-109-Eng-38. JEJ acknowledges funding from 2011 USDA PECASE award. NR 65 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD OCT 21 PY 2016 VL 9 AR 225 DI 10.1186/s13068-016-0639-2 PG 15 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA DZ8BA UT WOS:000386092200002 PM 27777626 ER PT J AU Jiang, Y Li, CY Takeda, F AF Jiang, Yu Li, Changying Takeda, Fumiomi TI Nondestructive Detection and Quantification of Blueberry Bruising using Near-infrared (NIR) Hyperspectral Reflectance Imaging SO SCIENTIFIC REPORTS LA English DT Article ID APPLES; CLASSIFICATION; FIRMNESS; QUALITY; FRUIT; TIME AB Currently, blueberry bruising is evaluated by either human visual/tactile inspection or firmness measurement instruments. These methods are destructive, time-consuming, and subjective. The goal of this paper was to develop a non-destructive approach for blueberry bruising detection and quantification. Experiments were conducted on 300 samples of southern highbush blueberry (Camellia, Rebel, and Star) and on 1500 samples of northern highbush blueberry (Bluecrop, Jersey, and Liberty) for hyperspectral imaging analysis, firmness measurement, and human evaluation. An algorithm was developed to automatically calculate a bruise ratio index (ratio of bruised to whole fruit area) for bruise quantification. The spectra of bruised and healthy tissues were statistically separated and the separation was independent of cultivars. Support vector machine (SVM) classification of the spectra from the regions of interest (ROIs) achieved over 94%, 92%, and 96% accuracy on the training set, independent testing set, and combined set, respectively. The statistical results showed that the bruise ratio index was equivalent to the measured firmness but better than the predicted firmness in regard to effectiveness of bruise quantification, and the bruise ratio index had a strong correlation with human assessment (R2 = 0.78 - 0.83). Therefore, the proposed approach and the bruise ratio index are effective to non-destructively detect and quantify blueberry bruising. C1 [Jiang, Yu; Li, Changying] Univ Georgia, Coll Engn, Athens, GA 30602 USA. [Takeda, Fumiomi] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Li, CY (reprint author), Univ Georgia, Coll Engn, Athens, GA 30602 USA. EM cyli@uga.edu FU USDA National Institute of Food and Agriculture Specialty Crop Research Initiative [2014-51181-22383] FX This study is supported by the USDA National Institute of Food and Agriculture Specialty Crop Research Initiative (Award No: 2014-51181-22383). The authors gratefully thank Ms. Renee Holland (University of Georgia Extension), and Mr. David Trinka and Mr. Rolland Groenick (Michigan Blueberry Growers) for their assistance in collecting blueberry samples used in this study, as well as Mr. Jesse Kuzy and Dr. Randy Beaudry's group (Mr. Patrick Abeli, Dr. Nihad Alsmairat, Mr. George Guimares, and Ms. Rosella Briano) for their assistance in conducting the experiments. The authors also especially thank Ms. Megan Lusher (UGA), Dr. Ann Rose and Mr. Jedediah Smith (USDA ARS) for providing an excellent technical assistance. NR 28 TC 0 Z9 0 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 21 PY 2016 VL 6 AR 35679 DI 10.1038/srep35679 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ4LX UT WOS:000385831300002 PM 27767050 ER PT J AU Krivan, V Lewis, M Bentz, BJ Bewick, S Lenhart, SM Liebhold, A AF Krivan, Vlastimil Lewis, Mark Bentz, Barbara J. Bewick, Sharon Lenhart, Suzanne M. Liebhold, Andrew TI A dynamical model for bark beetle outbreaks SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Bistability; Bark beetle; Dendroctonus ponderosae; Dispersal; Filippov solution; Hysteresis; Population dynamics; Stability; SI models ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; DENDROCTONUS-PONDEROSAE; SPATIOTEMPORAL PATTERNS; NATURAL DISTURBANCES; POPULATION-DYNAMICS; SPATIAL-PATTERNS; RANGE EXPANSION; FOREST; INSECT AB Tree-killing bark beetles are major disturbance agents affecting coniferous forest ecosystems. The role of environmental conditions on driving beetle outbreaks is becoming increasingly important as global climatic change alters environmental factors, such as drought stress, that, in turn, govern tree resistance. Furthermore, dynamics between beetles and trees are highly nonlinear, due to complex aggregation behaviors exhibited by beetles attacking trees. Models have a role to play in helping unravel the effects of variable tree resistance and beetle aggregation on bark beetle outbreaks. In this article we develop a new mathematical model for bark beetle outbreaks using an analogy with epidemiological models. Because the model operates on several distinct time scales, singular perturbation methods are used to simplify the model. The result is a dynamical system that tracks populations of uninfested and infested trees. A limiting case of the model is a discontinuous function of state variables, leading to solutions in the Filippov sense. The model assumes an extensive seed-bank so that tree recruitment is possible even if trees go extinct. Two scenarios are considered for immigration of new beetles. The first is a single tree stand with beetles immigrating from outside while the second considers two forest stands with beetle dispersal between them. For the seed-bank driven recruitment rate, when beetle immigration is low, the forest stand recovers to a beetle-free state. At high beetle immigration rates beetle populations approach an endemic equilibrium state. At intermediate immigration rates, the model predicts bistability as the forest can be in either of the two equilibrium states: a healthy forest, or a forest with an endemic beetle population. The model bistability leads to hysteresis. Interactions between two stands show how a less resistant stand of trees may provide an initial toe-hold for the invasion, which later leads to a regional beetle outbreak in the resistant stand. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Krivan, Vlastimil] Acad Sci Czech Republic, Inst Entomol, Ctr Biol, Branisovska 31, Ceske Budejovice 37005, Czech Republic. [Krivan, Vlastimil] Univ South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic. [Lewis, Mark] Univ Alberta, Dept Math & Stat Sci, Edmonton, AB T6G 2G1, Canada. [Bentz, Barbara J.] USFS Rocky Mt, Res Stn, 860 N 1200 East, Logan, UT 84321 USA. [Bewick, Sharon; Lenhart, Suzanne M.] Natl Inst Math & Biol Synth, 1534 White Ave, Knoxville, TN 37996 USA. [Liebhold, Andrew] USDA Forest Serv, 180 Canfield St, Morgantown, WV 26505 USA. RP Krivan, V (reprint author), Acad Sci Czech Republic, Inst Entomol, Ctr Biol, Branisovska 31, Ceske Budejovice 37005, Czech Republic. EM vlastimil.krivan@gmail.com; mark.lewis@ualberta.ca; bbentz@fs.fed.us; sharon.bewick@gmail.com; lenhart@math.utk.edu; aliebhold@fs.fed.us FU National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF Award [EF-0832858]; The University of Tennessee, Knoxville; Institute of Entomology [RVO:60077344]; NSERC Discovery and Accelerator grants; Canada Research Chair; Killam Fellowship; Natural Science and Engineering Research Council of Canada [NET GP 434810-12] FX This work was conducted as a part of the Forest Insect Pests Working Group at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Award #EF-0832858, with additional support from The University of Tennessee, Knoxville. V. K. acknowledges support provided by the Institute of Entomology (RVO:60077344). M.A.L. gratefully acknowledges support from NSERC Discovery and Accelerator grants, a Canada Research Chair and a Killam Fellowship. This research was also supported by a grant to MAL from the Natural Science and Engineering Research Council of Canada (grant no. NET GP 434810-12) to the TRIA Network, with contributions from Alberta Agriculture and Forestry, Foothills Research Institute, Manitoba Conservation and Water Stewardship, Natural Resources Canada Canadian Forest Service, Northwest Territories Environment and Natural Resources, Ontario Ministry of Natural Resources and Forestry, Saskatchewan Ministry of Environment, West Fraser and Weyerhaeuser. NR 48 TC 0 Z9 0 U1 44 U2 44 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 EI 1095-8541 J9 J THEOR BIOL JI J. Theor. Biol. PD OCT 21 PY 2016 VL 407 BP 25 EP 37 DI 10.1016/j.jtbi.2016.07.009 PG 13 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA DV7JI UT WOS:000383111800003 PM 27396358 ER PT J AU Park, KT ElNaggar, MM Abdellrazeq, GS Bannantine, JP Mack, V Fry, LM Davis, WC AF Park, Kun Taek ElNaggar, Mahmoud M. Abdellrazeq, Gaber S. Bannantine, John P. Mack, Victoria Fry, Lindsay M. Davis, William C. TI Phenotype and Function of CD209(+) Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages SO PLOS ONE LA English DT Article ID AVIUM SUBSP PARATUBERCULOSIS; RESPIRATORY SYNCYTIAL VIRUS; DC-SIGN; IMMUNE-RESPONSES; IDENTIFICATION; SUBPOPULATIONS; PATHOGENESIS; RECEPTOR; MUTANTS; ANTIGEN AB Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoF). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoF pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo. C1 [Park, Kun Taek; ElNaggar, Mahmoud M.; Abdellrazeq, Gaber S.; Mack, Victoria; Fry, Lindsay M.; Davis, William C.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Park, Kun Taek] Seoul Natl Univ, Dept Vet Microbiol, Coll Vet Med, Seoul 151742, South Korea. [ElNaggar, Mahmoud M.; Abdellrazeq, Gaber S.] Univ Alexandria, Fac Vet Med, Dept Microbiol, Alexandria, Egypt. [Bannantine, John P.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. [Fry, Lindsay M.] ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. RP Davis, WC (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM davisw@vetmed.wsu.edu RI Abdellrazeq, Gaber/C-7667-2015 OI Abdellrazeq, Gaber/0000-0002-8677-911X FU Washington State University Monoclonal Antibody Center; Egyptian government; USAID FX This study was supported by the Washington State University Monoclonal Antibody Center (http://vmp.vetmed.wsu.edu/resources/ monoclonal-antibody center). Mahmoud M. ElNaggar was supported in part by a fellowship from the Egyptian government. Gaber S. Abdellrazeq was supported by a fellowship from the USAID. NR 33 TC 1 Z9 1 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 20 PY 2016 VL 11 IS 10 AR e0165247 DI 10.1371/journal.pone.0165247 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9NZ UT WOS:000386204500140 PM 27764236 ER PT J AU Li, XR Zhang, FM Coates, B Zhang, YH Zhou, XG Cheng, DF AF Li, Xiangrui Zhang, Fangmei Coates, Brad Zhang, Yunhui Zhou, Xuguo Cheng, Dengfa TI Comparative profiling of microRNAs in the winged and wingless English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae) SO SCIENTIFIC REPORTS LA English DT Article ID SILKWORM BOMBYX-MORI; GENE-EXPRESSION; IMAGINAL DISCS; PEA APHID; DROSOPHILA; RNA; IDENTIFICATION; GROWTH; MORPHS; TRANSCRIPTION AB MicroRNAs (miRNAs) are short single-stranded non-coding RNAs that regulate gene expression, particularly during development. In this study, 345 miRNAs were identified from the English green aphid, Sitobion avenae (F.), of which 168 were conserved and 177 were S. avenae-specific. Quantitative comparison of miRNA expression levels indicated that 16 and 12 miRNAs were significantly up-regulated in winged and wingless S. avenae small RNA libraries, respectively. Differential expression of these miRNAs was confirmed by real-time quantitative RT-PCR validation. The putative transcript targets for these candidate miRNAs were predicted based on sequences from a model species Drosophila melanogaster and four aphid species Acyrthosiphon pisum, Myzus persicae, Toxoptera citricida, and Aphis gosspii. Gene Ontology and KEGG pathway analyses shed light on the potential functions of these miRNAs in the regulation of genes involved in the metabolism, development and wing polyphenism of S. avenae. C1 [Li, Xiangrui; Zhang, Fangmei; Zhang, Yunhui; Cheng, Dengfa] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Zhang, Fangmei] Xinyang Agr & Forestry Univ, Xinyang 464000, Henan, Peoples R China. [Coates, Brad] USDA ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Zhou, Xuguo] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. RP Cheng, DF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.; Zhou, XG (reprint author), Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. EM xuguozhou@uky.edu; dfcheng@ippcaas.cn FU National Natural Science Foundation of China [31301659]; National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China [2012BAD19B04]; China Agriculture Research System [CARS-3]; project of the Kentucky Agricultural Experiment Station [16-08-098] FX The authors are grateful to anonymous reviewers and editor for their constructive criticisms. This research was supported by the National Natural Science Foundation of China (Award number: 31301659), National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China (Award number: 2012BAD19B04), China Agriculture Research System (Award number: CARS-3). The information reported in this paper (No. 16-08-098) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 0 Z9 1 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 20 PY 2016 VL 6 AR 35668 DI 10.1038/srep35668 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9MU UT WOS:000386200300001 PM 27762301 ER PT J AU de Dios, VR Gessler, A Ferrio, JP Alday, JG Bahn, M del Castillo, J Devidal, S Garcia-Munoz, S Kayler, Z Landais, D Martin-Gomez, P Milcu, A Piel, C Pirhofer-Walzl, K Ravel, O Salekin, S Tissue, DT Tjoelker, MG Voltas, J Roy, J AF Resco de Dios, Victor Gessler, Arthur Pedro Ferrio, Juan Alday, Josu G. Bahn, Michael del Castillo, Jorge Devidal, Sebastien Garcia-Munoz, Sonia Kayler, Zachary Landais, Damien Martin-Gomez, Paula Milcu, Alexandru Piel, Clement Pirhofer-Walzl, Karin Ravel, Olivier Salekin, Serajis Tissue, David T. Tjoelker, Mark G. Voltas, Jordi Roy, Jacques TI Circadian rhythms have significant effects on leaf-to-canopy scale gas exchange under field conditions SO GIGASCIENCE LA English DT Article DE Circadian clock; Ecological memory; Net ecosystem exchange; Scaling; Stomatal conductance models; Photosynthesis; Transpiration ID CARBON-DIOXIDE EXCHANGE; STOMATAL CONDUCTANCE; WATER-USE; PHOTOSYNTHESIS; TRANSPIRATION; GROWTH; PLANTS; ASSIMILATION; ARABIDOPSIS; ATMOSPHERE AB Background: Molecular clocks drive oscillations in leaf photosynthesis, stomatal conductance, and other cell and leaf-level processes over similar to 24 h under controlled laboratory conditions. The influence of such circadian regulation over whole-canopy fluxes remains uncertain; diurnal CO2 and H2O vapor flux dynamics in the field are currently interpreted as resulting almost exclusively from direct physiological responses to variations in light, temperature and other environmental factors. We tested whether circadian regulation would affect plant and canopy gas exchange at the Montpellier European Ecotron. Canopy and leaf-level fluxes were constantly monitored under field-like environmental conditions, and under constant environmental conditions (no variation in temperature, radiation, or other environmental cues). Results: We show direct experimental evidence at canopy scales of the circadian regulation of daytime gas exchange: 20-79 % of the daily variation range in CO2 and H2O fluxes occurred under circadian entrainment in canopies of an annual herb (bean) and of a perennial shrub (cotton). We also observed that considering circadian regulation improved performance by 8-17 % in commonly used stomatal conductance models. Conclusions: Our results show that circadian controls affect diurnal CO2 and H2O flux patterns in entire canopies in field-like conditions, and its consideration significantly improves model performance. Circadian controls act as a 'memory' of the past conditions experienced by the plant, which synchronizes metabolism across entire plant canopies. C1 [Resco de Dios, Victor; Pedro Ferrio, Juan; Alday, Josu G.; del Castillo, Jorge; Martin-Gomez, Paula; Voltas, Jordi] Univ Lleida, Agrotecn Ctr, Dept Crop & Forest Sci, Lleida 25198, Spain. [Gessler, Arthur] Swiss Fed Inst Forest Snow & Landscape Res, Long Term Forest Ecosyst Res, CH-8903 Birmensdorf, Switzerland. [Gessler, Arthur; Kayler, Zachary; Pirhofer-Walzl, Karin] Leibniz Ctr Agr Landscape Res, Inst Landscape Biogeochem, D-15374 Muncheberg, Germany. [Pedro Ferrio, Juan] Univ Concepcion, Fac Ciencias Nat & Oceanog, Dept Bot, Casilla 160-C, Concepcion, Chile. [Alday, Josu G.] Univ Liverpool, Sch Environm Sci, Liverpool L69 3GP, Merseyside, England. [Bahn, Michael] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Devidal, Sebastien; Landais, Damien; Milcu, Alexandru; Piel, Clement; Ravel, Olivier; Roy, Jacques] CNRS, Ecotron Europeen Montpellier, UPS 3248, Campus Baillarguet, F-34980 Montferrier Sur Lez, France. [Garcia-Munoz, Sonia] Inst Madrileno Invest & Desarrollo Rural Agr & Al, Madrid 28800, Spain. [Kayler, Zachary] US Forest Serv, USDA, Northern Res Stn, Lawrence Livermore Natl Lab, Livermore, CA 94550 USA. [Milcu, Alexandru] Univ Montpellier, Univ Paul Valery, CNRS, Ctr Ecol Fonct & Evolut,Ecole Prat Hautes Etud,UM, F-34293 Montpellier 5, France. [Pirhofer-Walzl, Karin] Free Univ Berlin, Inst Biol Plant Ecol, D-14195 Berlin, Germany. [Pirhofer-Walzl, Karin] Berlin Brandenburg Inst Adv Biodivers Res, D-14195 Berlin, Germany. [Salekin, Serajis] Univ Lleida, Erasmus Mundus Master Mediterranean Forestry & Na, Lleida 25198, Spain. [Salekin, Serajis] Univ Canterbury, Coll Engn, Sch Forestry, Christchurch 8140, New Zealand. [Tissue, David T.; Tjoelker, Mark G.] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. RP de Dios, VR (reprint author), Univ Lleida, Agrotecn Ctr, Dept Crop & Forest Sci, Lleida 25198, Spain. EM v.rescodedios@gmail.com RI Gessler, Arthur/C-7121-2008; Ferrio, Juan Pedro/A-5748-2008; Tjoelker, Mark/M-2413-2016; Alday, Josu G./F-6175-2010; Resco de Dios, Victor/G-5555-2014 OI Gessler, Arthur/0000-0002-1910-9589; Ferrio, Juan Pedro/0000-0001-5904-7821; Tjoelker, Mark/0000-0003-4607-5238; Alday, Josu G./0000-0001-7510-8655; Resco de Dios, Victor/0000-0002-5721-1656 FU Ramon y Cajal fellowships [RYC-2012-10970, RYC-2008-02050]; Leibniz Centre for Agricultural Landscape Research; Western Sydney University's Hawkesbury Institute for the Environment; Spanish Government [AGL2015-69151-R] FX This study benefited from the CNRS human and technical resources allocated to the Research Infrastructure Ecotrons, as well as from the state allocation 'Investissement d'Avenir' ANR-11-INBS-0001; ExpeER Transnational Access program; Ramon y Cajal fellowships (RYC-2012-10970 to VRD and RYC-2008-02050 to JPF); the Erasmus Mundus Master Course Mediterranean Forestry and Natural Resources Management (MEDfOR); and internal grants from the Leibniz Centre for Agricultural Landscape Research to AG, and from the Western Sydney University's Hawkesbury Institute for the Environment and the Spanish Government (AGL2015-69151-R) to VRD. NR 56 TC 0 Z9 0 U1 21 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2047-217X J9 GIGASCIENCE JI GigaScience PD OCT 20 PY 2016 VL 5 AR 43 DI 10.1186/s13742-016-0149-y PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ1ZH UT WOS:000385640200001 ER PT J AU Xu, GL Singh, SK Reddy, VR Barnaby, JY Sicher, RC Li, T AF Xu, Guangli Singh, Shardendu K. Reddy, Vangimalla R. Barnaby, Jinyoung Y. Sicher, Richard C. Li, Tian TI Soybean grown under elevated CO2 benefits more under low temperature than high temperature stress: Varying response of photosynthetic limitations, leaf metabolites, growth, and seed yield SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Carbohydrate; Organic acids; Over compensation; Photosynthetic limitation; Quantum yield; Starch ID CARBON-DIOXIDE CONCENTRATION; MESOPHYLL CONDUCTANCE; ELECTRON-TRANSPORT; CHLOROPHYLL FLUORESCENCE; OXIDATIVE STRESS; GAS-EXCHANGE; PLANT-GROWTH; IN-VIVO; ENRICHMENT; RUBISCO AB To evaluate the combined effect of temperature and CO2 on photosynthetic processes, leaf metabolites and growth, soybean was grown under a controlled environment at low (22/18 degrees C, LT), optimum (28/24 degrees C, OT) and high (36/32 degrees C HT) temperatures under ambient (400 mu mol mol(-1); aCO(2)) or elevated (800 mu mol mol(-1); eCO(2)) CO2 concentrations during the reproductive stage. In general, the rate of photosynthesis (A), stomatal (g(s)) and mesophyll (g(m)) conductance, quantum yield of photosystem II, rates of maximum carboxylation (V-Cmax), and electron transport (J) increased with temperature across CO2 levels. However, compared with OT, the percentage increases in these parameters at HT were lower than the observed decline at LT. The photosynthetic limitation at LT and OT was primarily caused by photo-biochemical processes (49-58%, L-b) followed by stomatal (27-32%, L-s) and mesophyll (15-19%, L-m) limitations. However, at HT, it was primarily caused by L-s (41%) followed by L-b (33%) and L-m (26%). The dominance of L-b at LT and OT was associated with the accumulation of non-structural carbohydrates (e.g., starch) and several organic acids, whereas this accumulation did not occur at HT, indicating increased metabolic activities. Compared with OT, biomass and seed yield declined more at HT than at LT. The eCO(2) treatment compensated for the temperature-stress effects on biomass but only partially compensated for the effects on seed yield, especially at HT. Photosynthetic downregulation at eCO(2) was possibly due to the accumulation of non-structural carbohydrates and the decrease in g(s) and A(std) (standard A measured at 400 mu mol mol(-1) sub-stomatal CO2 concentration), as well as the lack of CO2 effect on g(m), V-Cmax,V- and J, and photosynthetic limitation. Thus, the photosynthetic limitation was temperature-dependent and was primarily influenced by the alteration in photo-biochemical processes and metabolic activities. Despite the inconsistent response of photosynthesis (or biomass accumulation) and seed yield, eCO(2) tended to fully or partially compensate for the adverse effect of the respective LT and HT stresses under well-watered and sufficient nutrient conditions. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Xu, Guangli; Singh, Shardendu K.; Reddy, Vangimalla R.; Barnaby, Jinyoung Y.; Sicher, Richard C.] ARS, Crop Syst & Global Change Lab, USDA, Bldg 001,Rm 342,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Xu, Guangli; Li, Tian] Sichuan Agr Univ, Coll Agron, Chengdu, Peoples R China. [Singh, Shardendu K.] Univ Maryland, Wye Res & Educ Ctr, College Pk, MD 20742 USA. RP Singh, SK (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Bldg 001,Rm 342,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM shardendu.singh@ars.usda.gov NR 61 TC 0 Z9 0 U1 30 U2 30 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 EI 1618-1328 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD OCT 20 PY 2016 VL 205 BP 20 EP 32 DI 10.1016/j.jplph.2016.08.003 PG 13 WC Plant Sciences SC Plant Sciences GA DZ4VL UT WOS:000385858600003 PM 27589223 ER PT J AU Yangcheng, H Belamkar, V Cannon, SB Jane, JL AF Yangcheng, Hanyu Belamkar, Vikas Cannon, Steven B. Jane, Jay-lin TI Characterization and development mechanism of Apios americana tuber starch SO CARBOHYDRATE POLYMERS LA English DT Article DE Apios; Mother tuber; Child tuber; Starch; Double thermal-transitiona ID PASTING PROPERTIES; MAIZE STARCH; GELATINIZATION; MEDIKUS; GROWTH; BARLEY; ACIDS AB Apios americana is a wild legume-bearing plant with edible tubers. Domestication of Apios is in progress because of the superior nutritional value and health benefits of the tuber. Objectives of this study were to: (1) characterize physicochemical properties of the Apios tuber starch; and (2) understand differences in starch structures and properties between the mother (seed) and child (progeny) tubers and the mechanism of starch development. Granules of the Apios tuber starch displayed ellipsoidal, rod, or kidney shape with diameter ranges of 1-30 mu m. The mother tuber starches displayed greater percentage crystallinity, larger gelatinization enthalpy-changes, longer branch-chain lengths of amylopectin, and lower pasting viscosity than their counterpart child tuber starches. The mother tuber starch of Apios 2127 displayed distinct two peaks of gelatinization, which were attributed to starch granules located at different regions of the tuber having different structures and properties. The mother tuber displayed more active starch biosynthesis in the periphery than in the center of the tuber. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yangcheng, Hanyu; Jane, Jay-lin] Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. [Belamkar, Vikas] Iowa State Univ, Interdept Genet, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Jane, JL (reprint author), Iowa State Univ, Dept Food Sci & Human Nutr, Ames, IA 50011 USA. EM jjane@iastate.edu NR 29 TC 1 Z9 1 U1 6 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 198 EP 205 DI 10.1016/j.carbpol.2016.05.062 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900022 PM 27474558 ER PT J AU Liu, YQ Wisniewski, M Kennedy, JF Jiang, YS Tang, JM Liu, J AF Liu, Yiqing Wisniewski, Michael Kennedy, John F. Jiang, Yusong Tang, Jianmin Liu, Jia TI Chitosan and oligochitosan enhance ginger (Zingiber officinale Roscoe) resistance to rhizome rot caused by Fusarium oxysporum in storage SO CARBOHYDRATE POLYMERS LA English DT Article DE Chitosan; Oligochitosan; Ginger rhizome; Defense response; Fusarium oxysporum ID POSTHARVEST DISEASES; FRUIT; ANTHRACNOSE; DEFENSE; QUALITY; DERIVATIVES; ANTIFUNGAL; PATHOGENS; RESPONSES; COATINGS AB The ability of chitosan and oligochitosan to enhance ginger (Zingiber officinale) resistance to rhizome rot caused by Fusarium oxysporum in storage was investigated. Both chitosan and oligochitosan at 1 and 5 g/L significantly inhibited rhizome rot, with the best control at 5 g/L. Chitosan and oligochitosan applied at 5 g/L also reduced weight loss, measured as a decrease in fresh weight, but did not affect soluble solids content or titratable acidity of rhizomes. The two compounds applied at 5 g/L induced beta-1,3-glucanase and phenylalanine ammonia-lyase enzyme activity and the transcript levels of their coding genes, as well as the total phenolic compounds in rhizome tissues. Therefore, the ability of chitosan and oligochitosan to reduce rot in stored rhizomes may be associated with their ability to induce defense responses in ginger. These results have practical implications for the application of chitosan and oligochitosan to harvested ginger rhizomes to reduce postharvest losses. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Liu, Yiqing; Jiang, Yusong; Tang, Jianmin; Liu, Jia] Chongqing Univ Arts & Sci, Coll Forestry & Life Sci, Inst Special Plants, Yongchuan 402160, Peoples R China. [Liu, Yiqing; Jiang, Yusong; Tang, Jianmin; Liu, Jia] Collabrat Innovatat Ctr Special Plant Ind Chongqi, Chongqing Engn Res Ctr Special Plant Seedlings, Garden & Flower Engn Res Ctr Chongqing Coll, Yongchuan 402160, Peoples R China. [Wisniewski, Michael] USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Kennedy, John F.] Chembiotech Labs, Kyrewood House, Tenbury Wells WR15 8SG, Worcs, England. RP Liu, J (reprint author), Chongqing Univ Arts & Sci, Coll Forestry & Life Sci, Inst Special Plants, Yongchuan 402160, Peoples R China. EM jialiu1983@163.com FU Scientific Research Fund of the Chongqing Education Commission [KJZH14216]; Scientific Research Fund of the Chongqing Science & Technology Commission [cstc2013jcyjA80035]; National Natural Science Foundation of China [31501273]; Chongqing Key Discipline Construction of Forestry FX This work was supported by the Scientific Research Fund of the Chongqing Education Commission (KJZH14216), the Scientific Research Fund of the Chongqing Science & Technology Commission (cstc2013jcyjA80035), National Natural Science Foundation of China (31501273), and the Chongqing Key Discipline Construction of Forestry. NR 39 TC 0 Z9 0 U1 20 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 474 EP 479 DI 10.1016/j.carbpol.2016.05.103 PG 6 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900055 PM 27474591 ER PT J AU Xie, JL Hse, CY De Hoop, CF Hu, TX Qi, JQ Shupe, TF AF Xie, Jiulong Hse, Chung-Yun De Hoop, Cornelis F. Hu, Tingxing Qi, Jinqiu Shupe, Todd F. TI Isolation and characterization of cellulose nanofibers from bamboo using microwave liquefaction combined with chemical treatment and ultrasonication SO CARBOHYDRATE POLYMERS LA English DT Article DE Cellulose nanofibers; Microwave liquefaction; Bamboo; Ultrasonic nanofibrillation ID MECHANICAL-PROPERTIES; ENZYMATIC-HYDROLYSIS; REINFORCING AGENT; WHEAT-STRAW; BANANA PEEL; NANOCRYSTALS; COMPOSITES; WOOD; EXTRACTION; COMPONENTS AB Cellulose nanofibers were successfully isolated from bamboo using microwave liquefaction combined with chemical treatment and ultrasonic nanofibrillation processes. The microwave liquefaction could eliminate almost all the lignin in bamboo, resulting in high cellulose content residues within 7 min, and the cellulose enriched residues could be readily purified by subsequent chemical treatments with lower chemical charging and quickly. The results of wet chemistry analyses, SEM images, and FTIR and X-ray spectra indicated the combination of microwave liquefaction and chemical treatment was significantly efficient in removing non-cellulosic compounds. Ultrasonication was used to separate the nanofibrils from the purified residues to extract nanofibers. The TEM images confirmed the presence of elementary fibrils, nano-sized fibril bundles, and aggregated fibril bundles. As evidenced by the TGA analysis, cellulose nanofibers isolated by this novel technique had high thermal stability indicating that the isolated nanofibers could possibly be applied as reinforcing elements in biomaterials. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Xie, Jiulong; Hu, Tingxing; Qi, Jinqiu] Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Xie, Jiulong; De Hoop, Cornelis F.; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resource, Baton Rouge, LA 70803 USA. RP Qi, JQ (reprint author), Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Sichuan, Peoples R China. EM qijinqiu2005@aliyun.com NR 57 TC 2 Z9 2 U1 42 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD OCT 20 PY 2016 VL 151 BP 725 EP 734 DI 10.1016/j.carbpol.2016.06.011 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DS7DV UT WOS:000380943900083 PM 27474619 ER PT J AU Bilbao-Sainz, C Chiou, BS Valenzuela-Medina, D Imam, SH Vega-Galvez, A Orts, WJ AF Bilbao-Sainz, Cristina Chiou, Bor-Sen Valenzuela-Medina, Diana Imam, Syed H. Vega-Galvez, Antonio Orts, William J. TI Biopolymer films to control fusarium dry rot and their application to preserve potato tubers SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE chitosan; fludioxonil; Fusarium solani; potatoes; sodium alginate ID EDIBLE CHITOSAN FILMS; DIFFERENT MOLECULAR-WEIGHTS; ANTIMICROBIAL ACTIVITY; LISTERIA-MONOCYTOGENES; FOODBORNE PATHOGENS; PHYSICAL-PROPERTIES; COMPOSITE FILMS; ESSENTIAL OIL; NANOPARTICLES; NATAMYCIN AB Films were cast using sodium alginate (NaAlg), high molecular weight (HMW) chitosan, and low molecular weight (LMW) chitosan as film forming biopolymers. Fludioxonil (Fl) at 1% concentration was used as fungicide. Thermal stability, mechanical, and water sorption properties of the films were examined. The effects of films on the Fusarium solani colony radial growth were evaluated in vitro and in potato tubers. Results showed that chitosan films were more thermally stable and less hydrophilic than alginate films. Addition of fluodioxonil to the films significantly reduced the film strength and increased the elongation at break as well as the film stiffness. In vitro studies showed that when fludioxonil was added to the formulation, NaAlg and Chitosan-LMW films had significantly higher antifungal activity (Fungistatic index = 56%) than Chitosan-HMW films (Fungistatic index = 50%). In vivo studies showed that Chitosan-LMW-1% Fl films delay the mycelial growth of F. solani in tubers kept at 25 degrees C for 2 weeks. (C) 2016 Wiley Periodicals, Inc. C1 [Bilbao-Sainz, Cristina; Chiou, Bor-Sen; Valenzuela-Medina, Diana; Imam, Syed H.; Orts, William J.] ARS, Bioprod Chem & Engn, USDA, WRRC, Albany, CA 94710 USA. [Vega-Galvez, Antonio] Univ La Serena, Dept Food Engn, Av Raul Bitran 1305, La Serena, Chile. RP Bilbao-Sainz, C (reprint author), ARS, Bioprod Chem & Engn, USDA, WRRC, Albany, CA 94710 USA. EM cristina.bilbao@ars.usda.gov RI Vega-Galvez, Antonio /F-1089-2015 NR 36 TC 0 Z9 0 U1 12 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD OCT 20 PY 2016 VL 133 IS 40 AR 44017 DI 10.1002/app.44017 PG 6 WC Polymer Science SC Polymer Science GA DS4AZ UT WOS:000380725100004 ER PT J AU Rasmussen-Ivey, CR Hossain, MJ Odom, SE Terhune, JS Hemstreet, WG Shoemaker, CA Zhang, DH Xu, DH Griffin, MJ Liu, YJ Figueras, MJ Santos, SR Newton, JC Liles, MR AF Rasmussen-Ivey, Cody R. Hossain, Mohammad J. Odom, Sara E. Terhune, Jeffery S. Hemstreet, William G. Shoemaker, Craig A. Zhang, Dunhua Xu, De-Hai Griffin, Matt J. Liu, Yong-Jie Figueras, Maria J. Santos, Scott R. Newton, Joseph C. Liles, Mark R. TI Classification of a Hypervirulent Aeromonas hydrophila Pathotype Responsible for Epidemic Outbreaks in Warm-Water Fishes SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Aeromonas hydrophila; pathogenesis; comparative genomics; emerging disease; bacteria; catfish; carp ID VI SECRETION SYSTEM; MOLECULAR CHARACTERIZATION; PHYLOGENETIC ANALYSIS; BACTERIAL GENOMES; DISEASE OUTBREAKS; SP-NOV.; SEQUENCE; AQUACULTURE; ANNOTATION; COMPONENTS AB Lineages of hypervirulent Aeromonas hydrophila (vAh) are the cause of persistent outbreaks of motile Aeromonas septicemia in warm water fishes worldwide. Over the last decade, this virulent lineage of A. hydrophila has resulted in annual losses of millions of tons of farmed carp and catfish in the People's Republic of China and the United States (US). Multiple lines of evidence indicate US catfish and Asian carp isolates of A. hydrophila affiliated with sequence type 251 (ST251) share a recent common ancestor. To address the genomic context for the putative intercontinental transfer and subsequent geographic spread of this pathogen, we conducted a core genome phylogenetic analysis on 61 Aeromonas spp. genomes, of which 40 were affiliated with A. hydrophila, with 26 identified as epidemic strains. Phylogenetic analyses indicate all ST251 strains form a coherent lineage affiliated with A. hydrophila. Within this lineage, conserved genetic loci unique to A. hydrophila were identified, with some genes present in consistently higher copy numbers than in non-epidemic A. hydrophila isolates. In addition, results from analyses of representative ST251 isolates support the conclusion that multiple lineages are present within US vAh isolated from Mississippi, whereas vAh isolated from Alabama appear clonal. This is the first report of genomic heterogeneity within US vAh isolates, with some Mississippi isolates showing closer affiliation with the Asian grass carp isolate ZC1 than other vAh isolated in the US. To evaluate the biological significance of the identified heterogeneity, comparative disease challenges were conducted with representatives of different vAh genotypes. These studies revealed that isolate ZC1 yielded significantly lower mortality in channel catfish, relative to Alabama and Mississippi vAh isolates. Like other Asian vAh isolates, the ZC1 lineage contains all core genes for a complete type VI secretion system (T6SS). In contrast, more virulent US isolates retain only remnants of the T6SS (clpB, hcp, vgrG, and vasH) which may have functional implications. Collectively, these results characterize a hypervirulent A. hydrophila pathotype that affects farmed fish on multiple continents. C1 [Rasmussen-Ivey, Cody R.; Hossain, Mohammad J.; Odom, Sara E.; Santos, Scott R.; Liles, Mark R.] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. [Terhune, Jeffery S.] Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL USA. [Hemstreet, William G.] Alabama Fish Farming Ctr, Greensboro, AL USA. [Shoemaker, Craig A.; Zhang, Dunhua; Xu, De-Hai] ARS, Aquat Anim Hlth Res Unit, USDA, Auburn, AL USA. [Griffin, Matt J.] Mississippi State Univ, Coll Vet Med, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS USA. [Liu, Yong-Jie] Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China. [Figueras, Maria J.] Univ Rovira & Virgili, Dept Ciencias Med Basicas, Fac Med & Ciencias Salud, IISPV, Reus, Spain. [Newton, Joseph C.] Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA. RP Liles, MR (reprint author), Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA.; Newton, JC (reprint author), Auburn Univ, Dept Pathobiol, Auburn, AL 36849 USA. EM newtojc@aubum.edu; lilesma@auburn.edu RI Santos, Scott/A-7472-2009 FU United States Department of Agriculture's Agriculture and Food Research Initiative [2013-67015-21313, MIS-371530]; USDA-ARS [6010-32000-026-00D]; Catfish Health Initiative; Aquavalens from the European Union Seventh Framework Program [311816]; Spanish Ministry of Science and Innovation [AGL2011-30461-C02-02, JPIW2013-095-CO3] FX This work was supported by a grant from the United States Department of Agriculture's Agriculture and Food Research Initiative (Projects #2013-67015-21313 and #MIS-371530) and the USDA-ARS (CRIS Project No. 6010-32000-026-00D and the Catfish Health Initiative). MF thanks the support by the projects: Aquavalens from the European Union Seventh Framework Program (FP7/2007-2013) under Grant agreement No.: 311816, and by projects from Spanish Ministry of Science and Innovation: AGL2011-30461-C02-02 and JPIW2013-095-CO3. NR 69 TC 1 Z9 1 U1 2 U2 2 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD OCT 18 PY 2016 VL 7 AR 1615 DI 10.3389/fmicb.2016.01615 PG 16 WC Microbiology SC Microbiology GA ED0JS UT WOS:000388529400001 PM 27803692 ER PT J AU Zhang, XH Yan, ZQ Li, XJ Lin, WC Dai, ZK Yan, YM Lu, PP Chen, WG Zhang, HM Chen, F Ma, JY Xie, QM AF Zhang, Xinheng Yan, Zhuanqiang Li, Xinjian Lin, Wencheng Dai, Zhenkai Yan, Yiming Lu, Piaopiao Chen, Weiguo Zhang, Huanmin Chen, Feng Ma, Jingyun Xie, Qingmei TI GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens SO ONCOTARGET LA English DT Article DE ALV-J; RNA-Seq; differentially expressed genes; GADD45 beta; viral replication ID RNA-SEQ; MYELOID LEUKOSIS; HPRS-103 STRAIN; MAREKS-DISEASE; LAYER FLOCKS; EXPRESSION; INTEGRATION; INFECTION; IDENTIFICATION; APOPTOSIS AB Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45 beta inhibited ALV-J replication. GADD45 beta could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections. C1 [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Yan, Zhuanqiang; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Xinheng; Li, Xinjian; Lin, Wencheng; Dai, Zhenkai; Yan, Yiming; Lu, Piaopiao; Chen, Weiguo; Chen, Feng; Ma, Jingyun; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Wencheng; Chen, Weiguo; Chen, Feng; Xie, Qingmei] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Natural Science Foundation of Guangdong Province [S2013030013313]; National Natural Science Foundation of China [31472217]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), National Natural Science Foundation of China (Grant No. 31472217) and Guangdong Province Science and Technology Plan Project (Grant Nos. 2012B020306002, 2012B091100078). NR 44 TC 0 Z9 0 U1 2 U2 2 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD OCT 18 PY 2016 VL 7 IS 42 BP 68883 EP 68893 DI 10.18632/oncotarget.12027 PG 11 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EB5UT UT WOS:000387446800091 PM 27655697 ER PT J AU Sezen, UU Barney, JN Atwater, DZ Pederson, GA Pederson, JF Chandler, JM Cox, TS Cox, S Dotray, P Kopec, D Smith, SE Schroeder, J Wright, SD Jiao, YN Kong, WQ Goff, V Auckland, S Rainville, LK Pierce, GJ Lemke, C Compton, R Phillips, C Kerr, A Mettler, M Paterson, AH AF Sezen, U. Uzay Barney, Jacob N. Atwater, Daniel Z. Pederson, Gary A. Pederson, Jeffrey F. Chandler, J. Mike Cox, T. Stan Cox, Sheila Dotray, Peter Kopec, David Smith, Steven E. Schroeder, Jill Wright, Steven D. Jiao, Yuannian Kong, Wenqian Goff, Valorie Auckland, Susan Rainville, Lisa K. Pierce, Gary J. Lemke, Cornelia Compton, Rosana Phillips, Christine Kerr, Alexandra Mettler, Matthew Paterson, Andrew H. TI Multi-Phase US Spread and Habitat Switching of a Post-Columbian Invasive, Sorghum halepense SO PLOS ONE LA English DT Article ID POPULATION-STRUCTURE; GENETIC-VARIATION; ADAPTIVE EVOLUTION; JOHNSON GRASS; L PERS; PLANT; RANGE; PROGRAM; GENOME AB Johnsongrass (Sorghum halepense) is a striking example of a post-Columbian founder event. This natural experiment within ecological time-scales provides a unique opportunity for understanding patterns of continent-wide genetic diversity following range expansion. Microsatellite markers were used for population genetic analyses including leaf-optimized Neighbor-Joining tree, pairwise FST, mismatch analysis, principle coordinate analysis, Tajima's D, Fu's F and Bayesian clusterings of population structure. Evidence indicates two geographically distant introductions of divergent genotypes, which spread across much of the US in <200 years. Based on geophylogeny, gene flow patterns can be inferred to have involved five phases. Centers of genetic diversity have shifted from two introduction sites separated by similar to 2000 miles toward the middle of the range, consistent with admixture between genotypes from the respective introductions. Genotyping provides evidence for a 'habitat switch' from agricultural to non-agricultural systems and may contribute to both Johnsongrass ubiquity and aggressiveness. Despite lower and more structured diversity at the invasion front, Johnsongrass continues to advance northward into cooler and drier habitats. Association genetic approaches may permit identification of alleles contributing to the habitat switch or other traits important to weed/invasive management and/or crop improvement. C1 [Sezen, U. Uzay; Kong, Wenqian; Goff, Valorie; Auckland, Susan; Rainville, Lisa K.; Pierce, Gary J.; Lemke, Cornelia; Compton, Rosana; Phillips, Christine; Kerr, Alexandra; Mettler, Matthew; Paterson, Andrew H.] Plant Genome Mapping Lab, Athens, GA 30602 USA. [Barney, Jacob N.; Atwater, Daniel Z.] Virginia Tech, Blacksburg, VA USA. [Pederson, Gary A.] ARS, USDA, PGRCU, Griffin, GA USA. [Pederson, Jeffrey F.] ARS, USDA, Lincoln, NE USA. [Pederson, Jeffrey F.] Univ Nebraska Lincoln, Lincoln, NE USA. [Chandler, J. Mike] Texas A&M Univ, College Stn, TX USA. [Cox, T. Stan; Cox, Sheila] Land Inst, Salina, KS USA. [Dotray, Peter] Texas Tech Univ, Lubbock, TX 79409 USA. [Kopec, David; Smith, Steven E.] Univ Arizona, Tucson, AZ USA. [Schroeder, Jill] New Mexico State Univ, Las Cruces, NM 88003 USA. [Wright, Steven D.] Univ Calif Davis, Davis, CA 95616 USA. [Jiao, Yuannian] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China. RP Paterson, AH (reprint author), Plant Genome Mapping Lab, Athens, GA 30602 USA. EM paterson@uga.edu OI Barney, Jacob/0000-0003-2949-5003 FU United States Department of Agriculture Biotechnology Risk Assessment Program [08-39211-19568, 2012-33522-19790]; United States Department of Agriculture Controlling Weedy and Invasive Plants program [2013-67013-21306] FX Funding was provided by the United States Department of Agriculture Biotechnology Risk Assessment Program (08-39211-19568 to AHP, 2012-33522-19790 to AHP and TSC), and the United States Department of Agriculture Controlling Weedy and Invasive Plants program (2013-67013-21306 to JNB and AHP). NR 46 TC 0 Z9 0 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 18 PY 2016 VL 11 IS 10 AR e0164584 DI 10.1371/journal.pone.0164584 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ9NV UT WOS:000386203800023 PM 27755565 ER PT J AU Li, YQ Liu, C Cui, Y Walse, SS Olver, R Zilberman, D Mitch, WA AF Li, Yuanqing Liu, Chong Cui, Yi Walse, Spencer S. Olver, Ryan Zilberman, David Mitch, William A. TI Development of an Activated Carbon-Based Electrode for the Capture and Rapid Electrolytic Reductive Debromination of Methyl Bromide from Postharvest Fumigations SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID DISINFECTION BY-PRODUCTS; CHLORINATED HYDROCARBONS; ELECTROCHEMICAL REACTOR; HALOGENATED FUMIGANTS; DEHALOGENATION; WATER; TRANSFORMATION; DECOMPOSITION; REGENERATION; THIOSULFATE AB Due to concerns surrounding its ozone depletion potential, there is a need for technologies to capture and destroy methyl bromide (CH3Br) emissions from postharvest fumigations applied to control agricultural pests. Previously, we described a system in which CH3Br fumes vented from fumigation chambers could be captured by granular activated carbon (GAC). The GAC was converted to a cathode by submergence in a high ionic strength solution and connection to the electrical grid, resulting in reductive debromination of the sorbed CH3Br. The GAC bed was drained and dried for reuse to capture and destroy CH3Br fumes from the next fumigation. However, the loose GAC particles and slow kinetics of this primitive electrode necessitated improvements. Here, we report the development of a cathode containing a thin layer of small GAC particles coating carbon cloth as a current distributor. Combining the high sorption potential of GAC for CH3Br with the conductivity of the carbon cloth current distributor, the cathode significantly lowered the total cell resistance and achieved 96% reductive debromination of CH3Br sorbed at 30% by weight to the GAC within IS h at -1 V applied potential vs standard hydrogen electrode, a time scale and efficiency suitable for postharvest fumigations. The cathode exhibited stable performance over 50 CH3Br capture and destruction cycles. Initial cost estimates indicate that this technique could treat CH3Br fumes at similar to$5/kg, roughly one-third of the cost of current alternatives. C1 [Li, Yuanqing; Mitch, William A.] Stanford Univ, Dept Civil & Environm Engn, 473 Via Ortega, Stanford, CA 94305 USA. [Liu, Chong; Cui, Yi] Stanford Univ, Dept Mat Sci & Engn, McCullough Bldg, Stanford, CA 94305 USA. [Walse, Spencer S.] ARS, San Joaquin Valley Agr Sci Ctr, USDA, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Olver, Ryan; Zilberman, David] Univ Calif Berkeley, Dept Agr & Resource Econ, Giannini Hall, Berkeley, CA 94720 USA. RP Mitch, WA (reprint author), Stanford Univ, Dept Civil & Environm Engn, 473 Via Ortega, Stanford, CA 94305 USA. EM wamitch@stanford.edu FU USDA Agricultural Research Service; USDA Foreign Agricultural Service; California Dried Plum Board under the Technical Assistance for Specialty Crops program [2010-19] FX This research was funded by the USDA Agricultural Research Service as well as the USDA Foreign Agricultural Service and the California Dried Plum Board under the Technical Assistance for Specialty Crops program, agreement #2010-19. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 10 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD OCT 18 PY 2016 VL 50 IS 20 BP 11200 EP 11208 DI 10.1021/acs.est.6b03489 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DZ5MW UT WOS:000385907200049 PM 27611209 ER PT J AU Lu, XL Kracher, B Saur, IML Bauer, S Ellwood, SR Wise, R Yaeno, T Maekawa, T Schulze-Lefert, P AF Lu, Xunli Kracher, Barbara Saur, Isabel M. L. Bauer, Saskia Ellwood, Simon R. Wise, Roger Yaeno, Takashi Maekawa, Takaki Schulze-Lefert, Paul TI Allelic barley MLA immune receptors recognize sequence-unrelated avirulence effectors of the powdery mildew pathogen SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE avirulence effectors; association analysis; R genes; plant-microbe interactions; powdery mildew ID F-SP HORDEI; INTEGRATIVE GENOMICS VIEWER; DISEASE RESISTANCE GENES; MAGNAPORTHE-ORYZAE; PLANT-PATHOGENS; NLR FUNCTIONS; EVOLUTION; WHEAT; LOCUS; CONSERVATION AB Disease-resistance genes encoding intracellular nucleotide-binding domain and leucine-rich repeat proteins (NLRs) are key components of the plant innate immune system and typically detect the presence of isolate-specific avirulence (AVR) effectors from pathogens. NLR genes define the fastest-evolving gene family of flowering plants and are often arranged in gene clusters containing multiple paralogs, contributing to copy number and allele-specific NLR variation within a host species. Barley mildew resistance locus a (Mla) has been subject to extensive functional diversification, resulting in allelic resistance specificities each recognizing a cognate, but largely unidentified, AVR(a) gene of the powdery mildew fungus, Blumeria graminis f. sp. hordei (Bgh). We applied a transcriptome-wide association study among 17 Bgh isolates containing different AVR(a) genes and identified AVR(a1) and AVR(a13), encoding candidate-secreted effectors recognized by Mla1 and Mla13 alleles, respectively. Transient expression of the effector genes in barley leaves or protoplasts was sufficient to trigger Mla1 or Mla13 allele-specific cell death, a hallmark of NLR receptor-mediated immunity. AVR(a1) and AVR(a13) are phylogenetically unrelated, demonstrating that certain allelic MLA receptors evolved to recognize sequence-unrelated effectors. They are ancient effectors because corresponding loci are present in wheat powdery mildew. AVRA1 recognition by barley MLA1 is retained in transgenic Arabidopsis, indicating that AVR(A1) directly binds MLA1 or that its recognition involves an evolutionarily conserved host target of AVR(A1). Furthermore, analysis of transcriptome-wide sequence variation among the Bgh isolates provides evidence for Bgh population structure that is partially linked to geographic isolation. C1 [Lu, Xunli; Kracher, Barbara; Saur, Isabel M. L.; Bauer, Saskia; Maekawa, Takaki; Schulze-Lefert, Paul] Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany. [Ellwood, Simon R.] Curtin Univ, Dept Environm & Agr, Ctr Crop & Dis Management, Bentley, WA 6102, Australia. [Wise, Roger] ARS, Corn Insects & Crop Genet Res, USDA, Ames, IA 50011 USA. [Wise, Roger] Iowa State Univ, Ctr Plant Responses Environm Stresses, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Yaeno, Takashi] Ehime Univ, Fac Agr, Matsuyama, Ehime 7908566, Japan. RP Maekawa, T; Schulze-Lefert, P (reprint author), Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany. EM maekawa@mpipz.mpg.de; schlef@mpipz.mpg.de FU Max-Planck Society; German Research Foundation in the Collaborative Research Centre [SFB670]; Grains Research AMP; Development Corporation [CUR00017, CUR00023 P8]; JSPS KAKENHI Grant Inamori Foundation [JP16K07618] FX We thank L. Chartrain and J. Brown (John Innes Centre) for providing the Bgh isolates DH14, CC1, CC52, CC66, CC88, CC107, and CC148; M. F. Lyngkjaer (University of Copenhagen) for Bgh isolate B103; R. Oliver (Curtin University) for the Bgh isolates Aby, Art, and Will; H. Hisano (Okayama University) for the Bgh isolate OU14; N. Yamaoka (Ehime University) for the Bgh isolate RACE1; and M. Moscou (The Sainsbury Laboratory) for providing the Manchuria NIL lines. The ZmUbi-LUC plasmid, pIPKb002, and R4pGWB4-stop-HSP were kindly provided by R. Panstruga (RWTH Aachen University), J. Kumlehn (Leibniz Institute of Plant Genetics and Crop Plant Research), and N. Mitsuda (National Institute of Advanced Industrial Science and Technology), respectively. We thank C. Pedersen (University of Copenhagen) for helpful suggestions. We also thank S. Haigis and M. Yoshikawa-Maekawa for maintaining the Bgh isolates; P. Kochner for technical assistance; and the Max Planck Genome Centre Cologne for RNA-seq. This work was supported by the Max-Planck Society (X.L., B.K., I. M. L. S., S.B. and P. S.-L.); German Research Foundation in the Collaborative Research Centre Grant SFB670 (to B.K., T.M., and P. S.-L.); Grains Research & Development Corporation Projects CUR00017 and CUR00023 P8 (to S.R.E.); and the JSPS KAKENHI Grant JP16K07618 Inamori Foundation (to T.Y.). NR 80 TC 2 Z9 2 U1 20 U2 20 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 18 PY 2016 VL 113 IS 42 BP E6486 EP E6495 DI 10.1073/pnas.1612947113 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ1PN UT WOS:000385610400022 PM 27702901 ER PT J AU Maggioli, MF Palmer, MV Thacker, TC Vordermeier, HM McGill, JL Whelan, AO Larsen, MH Jacobs, WR Waters, WR AF Maggioli, Mayara F. Palmer, Mitchell V. Thacker, Tyler C. Vordermeier, Hans Martin McGill, Jodi L. Whelan, Adam O. Larsen, Michelle H. Jacobs, William R., Jr. Waters, W. Ray TI Increased TNF-alpha/IFN-gamma/IL-2 and Decreased TNF-alpha/IFN-gamma Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination SO FRONTIERS IN IMMUNOLOGY LA English DT Article DE polyfunctional T cells; central memory T cells; bovine tuberculosis; calf model ID CALMETTE-GUERIN VACCINATION; MYCOBACTERIUM-BOVIS; HISTORICAL PERSPECTIVES; ANTIGENS ESAT-6; INFECTED CATTLE; FUSION PROTEIN; EFFECTOR; VACCINES; GENERATION; DIFFERENTIATION AB Central memory T cell (Tcm) and polyfunctional CD4 T cell responses contribute to vaccine-elicited protection with both human and bovine tuberculosis (TB); however, their combined role in protective immunity to TB is unclear. To address this question, we evaluated polyfunctional cytokine responses by CD4 T cell effector/memory populations from bacille Calmette-Guerin (BCG) vaccinated and non-vaccinated calves by flow cytometry prior to and after aerosol challenge with virulent Mycobacterium bovis. Polyfunctional cytokine expression patterns in the response by Tcm, effector memory, and effector T cell subsets were similar between BCG-vaccinated and M. bovis-infected calves, only differing in magnitude (i.e., infected > vaccinated). BCG vaccination, however, did alter the kinetics of the ensuing response to virulent M. bovis infection. Early after challenge (3 weeks post-infection), non-vaccinates had greater antigen-specific interferon-7 (IFN-7)/tumor necrosis factor-alpha (TNIF-alpha) and lesser IFN-7/TNF-alpha/IL-2 responses by Tcm cells than did vaccinated animals. Importantly, these differences were also associated with mycobacterial burden upon necropsy. Polyfunctional responses to ESAT-6:CFP10 (antigens not synthesized by BCG strains) were detected in memory subsets, as well as in effector cells, as early as 3 weeks after challenge. These findings suggest that cell fate divergence may occur early after antigen priming in the response to bovine TB and that memory and effector T cells may expand concurrently during the initial phase of the immune response. In summary, robust IFN-gamma/TNIF-alpha response by Tom cells is associated with greater mycobacterial burden, while IFN-7/INF-alpha/IL-2 response by Tcm cells are indicative of a protective response to bovine TB. C1 [Maggioli, Mayara F.; Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. [Maggioli, Mayara F.] Iowa State Univ, Coll Vet Med, Dept Vet Pathol, Imbio, Ames, IA 50011 USA. [Vordermeier, Hans Martin] Anim & Plant Hlth Agcy, TB Res Grp, Addlestone, Surrey, England. [McGill, Jodi L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathol, Manhattan, KS 66506 USA. [Whelan, Adam O.] Def Sci & Technol Lab, Porton Down, Wilts, England. [Larsen, Michelle H.; Jacobs, William R., Jr.] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA. RP Maggioli, MF (reprint author), Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA.; Maggioli, MF (reprint author), Iowa State Univ, Coll Vet Med, Dept Vet Pathol, Imbio, Ames, IA 50011 USA. EM mayaramaggioli@gmail.com RI Vordermeier, H Martin/C-6936-2011; APHA, Staff publications/E-6082-2010 FU AFRI Competitive Grant [2011-67015-30736]; NIFA-USDA-NIH program Dual Purpose; Dual Benefit Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Animals FX This study was partially supported by AFRI Competitive Grant #2011-67015-30736 to WW and MP from the NIFA-USDA-NIH program Dual Purpose with Dual Benefit Research in Biomedicine and Agriculture Using Agriculturally Important Domestic Animals. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. NR 85 TC 0 Z9 0 U1 6 U2 6 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-3224 J9 FRONT IMMUNOL JI Front. Immunol. PD OCT 17 PY 2016 VL 7 AR 421 DI 10.3389/fimmu.2016.00421 PG 18 WC Immunology SC Immunology GA DY8XX UT WOS:000385416800002 PM 27799930 ER PT J AU Chiu, CJ Chang, ML Taylor, A AF Chiu, Chung-Jung Chang, Min-Lee Taylor, Allen TI Associations between Periodontal Microbiota and Death Rates SO SCIENTIFIC REPORTS LA English DT Article ID NUTRITION EXAMINATION SURVEY; ALL-CAUSE MORTALITY; NATIONAL-HEALTH; PORPHYROMONAS-GINGIVALIS; CARDIOVASCULAR-DISEASE; DIABETES-MELLITUS; OXIDATIVE STRESS; SERUM ANTIBODY; ORAL HYGIENE; NHANES-III AB It is conceived that specific combinations of periodontal bacteria are associated with risk for the various forms of periodontitis. We hypothesized that such specificity is also related to human cause-specific death rates. We tested this hypothesis in a representative sample of the US population followed for a mean duration of 11 years and found that two specific patterns of 21 serum antibodies against periodontal bacteria were significantly associated with increased all-cause and/or diabetes-related mortalities. These data suggested that specific combinations of periodontal bacteria, even without inducing clinically significant periodontitis, may have a significant impact on human cause-specific death rates. Our findings implied that increased disease and mortality risk could be transmittable via the transfer of oral microbiota, and that developing personalized strategies and maintaining healthy oral microbiota beyond protection against periodontitis would be important to manage the risk. C1 [Chiu, Chung-Jung; Chang, Min-Lee; Taylor, Allen] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chiu, CJ (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM CJ.Chiu@tufts.edu FU National Institutes of Health [RO1EY021826, RO1EY013250, RO1EY021212]; United States Department of Agriculture [1950-5100-060-01A] FX Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. Financial support for this project has been provided by the RO1EY021826 (C.-J.C.), RO1EY013250 and RO1EY021212 (A.T.) from the National Institutes of Health, and the United States Department of Agriculture under agreements, 1950-5100-060-01A. We thank Yu-Hsuan Chiu for her help with proofreading this manuscript. NR 53 TC 0 Z9 0 U1 4 U2 4 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 17 PY 2016 VL 6 AR 35428 DI 10.1038/srep35428 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY9LI UT WOS:000385454800001 PM 27748442 ER PT J AU Ren, JS Murphy, G Fan, JH Dawsey, SM Taylor, PR Selhub, J Qiao, YL Abnet, CC AF Ren, Jiansong Murphy, Gwen Fan, Jinhu Dawsey, Sanford M. Taylor, Philip R. Selhub, Jacob Qiao, Youlin Abnet, Christian C. TI Prospective study of serum B vitamins levels and oesophageal and gastric cancers in China SO SCIENTIFIC REPORTS LA English DT Article ID NUTRITION INTERVENTION; GENERAL-POPULATION; DIETARY-FOLATE; HUMAN-PLASMA; RISK; RIBOFLAVIN; COHORT; SUPPLEMENTATION; TRIAL; HOMOCYSTEINE AB B vitamins play an essential role in DNA synthesis and methylation, and may protect against oesophageal and gastric cancers. In this case-cohort study, subjects were enrolled from the General Population Nutrition Intervention Trial in Linxian, China. Subjects included 498 oesophageal squamous cell carcinomas (OSCCs), 255 gastric cardia adenocarcinomas (GCAs), and an age-and sex-matched sub-cohort of 947 individuals. Baseline serum riboflavin, pyridoxal phosphate (PLP), folate, vitamin B12, and flavin mononucleotide (FMN) were measured for all subjects. We estimated the associations with Cox proportional hazard models, with adjustment for potential confounders. Compared to those in the lowest quartile of serum riboflavin, those in the highest had a 44% lower risk of OSCC (HR: 0.56, 95% CI: 0.41 to 0.75). Serum vitamin B12 as a continuous variable was observed to be significantly inversely associated with OSCC (HR: 0.95, 95% CI: 0.89 to 1.01, P for score test = 0.041). Higher serum FMN levels were significantly associated with increased risk of OSCC (HR: 1.08, 95% CI: 1.01 to 1.16) and GCA (HR: 1.09, 95% CI: 1.00 to 1.20). Our study prompted that B vitamins have the potential role as chemopreventive agents for upper gastrointestinal cancers. C1 [Ren, Jiansong] Chinese Acad Med Sci, Natl Canc Ctr, Canc Hosp, Program Off Canc Screening Urban China, Beijing, Peoples R China. [Ren, Jiansong; Fan, Jinhu; Qiao, Youlin] Peking Union Med Coll, Beijing, Peoples R China. [Ren, Jiansong; Murphy, Gwen; Dawsey, Sanford M.; Taylor, Philip R.; Abnet, Christian C.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA. [Fan, Jinhu; Qiao, Youlin] Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Epidemiol, Beijing, Peoples R China. [Selhub, Jacob] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Qiao, YL (reprint author), Peking Union Med Coll, Beijing, Peoples R China.; Abnet, CC (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.; Qiao, YL (reprint author), Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Dept Epidemiol, Beijing, Peoples R China. EM qiaoy@cicams.ac.cn; abnetc@mail.nih.gov RI Abnet, Christian/C-4111-2015 OI Abnet, Christian/0000-0002-3008-7843 FU Intramural Research Program of the Division of Cancer Epidemiology and Genetics of the National Cancer Institute, NIH; NIH [N01-SC-91030, HHSN261200477001C]; National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences FX This work was supported in part by the Intramural Research Program of the Division of Cancer Epidemiology and Genetics of the National Cancer Institute, NIH, and in part by NIH contracts N01-SC-91030 and HHSN261200477001C with the National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences. NR 27 TC 0 Z9 0 U1 3 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 17 PY 2016 VL 6 AR 35281 DI 10.1038/srep35281 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY8CI UT WOS:000385355700002 PM 27748414 ER PT J AU Mukhopadhyay, S Sokorai, K Ukuku, D Fan, XT Juneja, V Sites, J Cassidy, J AF Mukhopadhyay, Sudarsan Sokorai, Kimberly Ukuku, Dike Fan, Xuetong Juneja, Vijay Sites, Joseph Cassidy, Jennifer TI Inactivation of Salmonella enterica and Listeria monocytogenes in cantaloupe puree by high hydrostatic pressure with/without added ascorbic acid SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Cantaloupe puree; High hydrostatic pressure processing; Salmonella enterica; Listeria monocytogenes; Antimicrobial ID ESCHERICHIA-COLI O157H7; FRESH-CUT PIECES; STRAWBERRY PUREE; FRUIT JUICES; SUBLETHAL INJURY; CHERRY TOMATO; STORAGE; APPLE; SURVIVAL; RIND AB The objective of this research was to evaluate and develop a method for inactivation of Salmonella enterica and Listeria monocytogenes in cantaloupe puree (CP) by high hydrostatic pressure (HHP). Cantaloupe being the most netted varieties of melons presents a greater risk of pathogen transmission. Freshly prepared CP with or without 0.1% ascorbic acid (AA) was inoculated with a bacterial cocktail composed of a three serotype mixture of S. enterica (S. Poona, S. Newport H1275 and S. Stanley H0558) and a mixture of three strains of L. monocytogenes (Scott A, 43256 and 51742) to a population of ca. 10(8) CFU/g. Double sealed and double bagged inoculated CP (ca. 5 g) were pressure treated at 300,400 and 500 MPa at 8 degrees C and 15 degrees C for 5 min. Data indicated increased inactivation of both Salmonella and Listeria spp. with higher pressure. Log reduction for CP at 300 MPa, 8 degrees C for 5 min was 2.4 +/- 0.2 and 1.6 +/- 0.5 log CFU/g for Salmonella and Listeria, respectively. Survivability of the pathogens was significantly compromised at 400 MPa and 8 degrees C, inactivating 4.5 +/- 0.3 log CFU/g of Salmonella and 3.0 +/- 0.4 log CFU/g of Listeria spp. Complete inactivation of the pathogens in the puree (log reduction >6.7 log CFU/g), with or without AA, was achieved when the pressure was further increased to 500 MPa, except that for Listeria containing no AA at 8 degrees C. Listeria presented higher resistance to pressure treatment compared to Salmonella spp. Initial temperatures (8 and 15 degrees C) had no significant influence on Salmonella log reductions. Log reduction of pathogens increased but not significantly with increase of temperature. AA did not show any significant antimicrobial activity. Viable counts were about 0.2-0.4 log CFU/g less in presence of 0.1% AA. These data validate that HHP can be used as an effective method for decontamination of cantaloupe puree. Published by Elsevier B.V. C1 [Mukhopadhyay, Sudarsan; Sokorai, Kimberly; Fan, Xuetong; Juneja, Vijay] ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Ukuku, Dike; Sites, Joseph; Cassidy, Jennifer] ARS, Food Safety Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mukhopadhyay, S (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM Sudarsan.mukhopadhyay@ars.usda.gov; Kimberly.sokorai@ars.usda.gov; Dike.ukuku@ars.usda.gov; Xuetong.fan@ars.usda.gov; Vijay.juneja@ars.usda.gov; Joseph.sites@ars.usda.gov; jennifer.cassidy@ars.usda.gov FU USDA through ARS National Program [108]; ARS CRIS project [1935-41000-092] FX The authors thank Jia Zhen for the help with high pressure experiments and Dr. John Phillips for assistance with statistical data analysis. This study was funded by the USDA through ARS National Program 108, ARS CRIS project 1935-41000-092. NR 59 TC 1 Z9 1 U1 20 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD OCT 17 PY 2016 VL 235 BP 77 EP 84 DI 10.1016/j.ijfoodmicro.2016.07.007 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DW7IB UT WOS:000383823300010 PM 27441819 ER PT J AU Sayols-Baixeras, S Subirana, I Lluis-Ganella, C Civeira, F Roquer, J Do, AN Absher, D Cenarro, A Munoz, D Soriano-Tarraga, C Jimenez-Conde, J Ordovas, JM Senti, M Aslibekyan, S Marrugat, J Arnett, DK Elosua, R AF Sayols-Baixeras, S. Subirana, I. Lluis-Ganella, C. Civeira, F. Roquer, J. Do, A. N. Absher, D. Cenarro, A. Munoz, D. Soriano-Tarraga, C. Jimenez-Conde, J. Ordovas, J. M. Senti, M. Aslibekyan, S. Marrugat, J. Arnett, D. K. Elosua, R. TI Identification and validation of seven new loci showing differential DNA methylation related to serum lipid profile: an epigenome-wide approach. The REGICOR study SO HUMAN MOLECULAR GENETICS LA English DT Article ID FAMILIAL COMBINED HYPERLIPIDEMIA; DENSITY-LIPOPROTEIN CHOLESTEROL; THIOREDOXIN INTERACTING PROTEIN; BODY-MASS INDEX; WAIST CIRCUMFERENCE; DIABETES-MELLITUS; LOWERING DRUGS; GENE PROMOTER; DIET NETWORK; BLOOD-LIPIDS AB Lipid traits (total, low-density and high-density lipoprotein cholesterol, and triglycerides) are risk factors for cardiovascular disease. DNA methylation is not only an inherited but also modifiable epigenetic mark that has been related to cardiovascular risk factors. Our aim was to identify loci showing differential DNA methylation related to serum lipid levels. Blood DNA methylation was assessed using the Illumina Human Methylation 450 BeadChip. A two-stage epigenome-wide association study was performed, with a discovery sample in the REGICOR study (n = 645) and validation in the Framingham Offspring Study (n = 2,542). Fourteen CpG sites located in nine genes (SREBF1, SREBF2, PHOSPHO1, SYNGAP1, ABCG1, CPT1A, MYLIP, TXNIP and SLC7A11) and 2 intergenic regions showed differential methylation in association with lipid traits. Six of these genes and 1 intergenic region were new discoveries showing differential methylation related to total cholesterol (SREBF2), HDLcholesterol (PHOSPHO1, SYNGAP1 and an intergenic region in chromosome 2) and triglycerides (MYLIP, TXNIP and SLC7A11). These CpGs explained 0.7%, 9.5% and 18.9% of the variability of total cholesterol, HDL cholesterol and triglycerides in the Framingham Offspring Study, respectively. The expression of the genes SREBF2 and SREBF1 was inversely associated with methylation of their corresponding CpGs (P-value = 0.0042 and 0.0045, respectively) in participants of the GOLDN study ( n = 98). In turn, SREBF1 expression was directly associated with HDL cholesterol ( P-value = 0.0429). Genetic variants in SREBF1, PHOSPHO1, ABCG1 and CPT1A were also associated with lipid profile. Further research is warranted to functionally validate these new loci and assess the causality of new and established associations between these differentially methylated loci and lipid metabolism. C1 [Sayols-Baixeras, S.; Subirana, I.; Lluis-Ganella, C.; Marrugat, J.; Elosua, R.] Hosp del Mar, Med Res Inst, IMIM, Cardiovasc Epidemiol & Genet Res Grp, Barcelona, Catalonia, Spain. [Sayols-Baixeras, S.] UPF, Barcelona, Catalonia, Spain. [Subirana, I.] CIBER Epidemiol & Publ Hlth, Barcelona, Catalonia, Spain. [Civeira, F.; Cenarro, A.] Hosp Univ Miguel Servet, Inst Invest Sanitaria Aragon, Unidad Clin & Invest Lipidos & Arteriosclerosis, Zaragoza, Spain. [Roquer, J.; Soriano-Tarraga, C.; Jimenez-Conde, J.] Hosp del Mar, Med Res Inst, IMIM, Neurovasc Res Grp,Dept Neurol, Barcelona 08003, Catalonia, Spain. [Do, A. N.; Aslibekyan, S.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Absher, D.] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Munoz, D.] Hosp del Mar, Med Res Inst, IMIM, Cardiovasc Risk & Nutr Res Grp, Barcelona, Catalonia, Spain. [Ordovas, J. M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Senti, M.] Pompeu Fabra Univ, Dept Expt & Hlth Sci, Barcelona, Catalonia, Spain. [Arnett, D. K.] Univ Kentucky, Coll Publ Hlth, Deans Off, Lexington, KY USA. RP Elosua, R (reprint author), Hosp del Mar, IMIM, Med Res Inst, Dr Aiguader 80, Barcelona 08003, Catalonia, Spain. EM relosua@imim.es FU Agencia de Gestio Ajuts Universitaris de Recerca [2014 SGR 240]; Spanish Ministry of Economy through the Carlos III Health Institute [ISCIII-FIS-FEDER-ERDF PI12-00232, PI12-01238, PI11-01801, PI08-1327, PI05-1251, PI05-1297, PI02-0471, FIS99/0013-01, FIS96/0026-01, FIS93/0568, FIS92/0009-05]; Red de Investigacion Cardiovascular [RD12/0042/0013, RD12/0042/0020, RD12/0042/0055, RD12/0042/0061]; Instituto de Salud Carlos III FEDER [IFI14/00007]; Daniel Bravo Andreu Private Foundation; US National Institute of Health (NIH)/National Heart, Lung and Blood Institutes [R01HL104135, U01HL72524] FX This work was supported by the following sources: Agencia de Gestio Ajuts Universitaris de Recerca [2014 SGR 240]; the Spanish Ministry of Economy through the Carlos III Health Institute [ISCIII-FIS-FEDER-ERDF PI12-00232, PI12-01238, PI11-01801, PI08-1327, PI05-1251, PI05-1297, PI02-0471, FIS99/0013-01, FIS96/0026-01, FIS93/0568, FIS92/0009-05], and the Red de Investigacion Cardiovascular [RD12/0042/0013, RD12/0042/0020, RD12/0042/0055, RD12/0042/0061]. S.S-B. was funded by a contract from Instituto de Salud Carlos III FEDER [IFI14/00007] and Daniel Bravo Andreu Private Foundation.; GOLDN: The GOLDN study (AND, DA, JO, SA, DKA) was funded by the US National Institute of Health (NIH)/National Heart, Lung and Blood Institutes (http://www.nhlbi.nih.gov) grants R01HL104135 and U01HL72524. NR 52 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 EI 1460-2083 J9 HUM MOL GENET JI Hum. Mol. Genet. PD OCT 15 PY 2016 VL 25 IS 20 BP 4556 EP 4565 DI 10.1093/hmg/ddw285 PG 10 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA EN1ZM UT WOS:000395809100016 PM 28173150 ER PT J AU Dubey, JP Brown, J Ternent, M Verma, SK Hill, DE Cerqueira-Cezar, CK Kwok, OCH Calero-Bernal, R Humphreys, JG AF Dubey, Jitender P. Brown, Justin Ternent, Mark Verma, Shiv K. Hill, Dolores E. Cerqueira-Cezar, Camila K. Kwok, Oliver C. H. Calero-Bernal, Rafael Humphreys, Jan G. TI Seroepidemiologic study on the prevalence of Toxoplasma gondii and Trichinella spp. infections in black bears (Ursus americanus) in Pennsylvania, USA SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Trichinella spp.; ELISA; Modified agglutination test; Epidemiology; Black bear (Ursus americanus) ID UNITED-STATES; SEROLOGIC TESTS; TRICHINOSIS; MURRELLI; CALIFORNIA; SPIRALIS; WILDLIFE AB The protozoan Toxoplasma gondii and the metazoan Trichinella spp. infect virtually all warm-blooded animals, including birds, humans, livestock, and marine mammals. Both parasitic infections can cause serious illness in human beings and can be acquired by ingesting under-cooked meat harboring infective stages. Approximately 3500 black bears (Ursus americanus) are legally-harvested each year in Pennsylvania, USA during the November hunting season. Among animals found infected with T. gondii, the prevalence of T. gondii is the highest among black bears in the USA; however, little is currently known of epidemiology of toxoplasmosis in this host species. Serum samples were collected during the winters of 2015 and 2016 from adult female bears and their nursing cubs or yearlings while they were still in their dens. Additionally, archived sera from bear samples collected throughout the year, including hunter-harvested bears in November and trapped bears in the summer, were serologically tested. Antibodies to T. gondii were assayed by the modified agglutination test (MAT, cut-off 1:25) and antibodies to Trichinella spp. were assayed using a commercial enzyme-linked immunosorbent assay (ELISA). Overall, T. gondii antibodies were found in 87.6% (206/235) of adults, and 44.1% (30/68) of yearlings. In March 2015/2016 sampling, antibodies to T. gondii were found in 94% (30/32) adult female bears while in their den. Antibodies were detected in 5% (3/66) of the nursing cubs in the dens of these sows. One positive cub had a MAT titer of 1:160 and two were positive at the 1:25 dilution but not at 1:50. The adult females of these cubs had MAT titers ranging from 1:400 to 1:3200. Antibodies to Trichinella spp. were found in 3% (6/181) of adults and 3.6% (1/28) of yearlings; these 7 bears were also seropositive for T. gondii. No antibodies to Trichinella spp. were detected in the sera of 44 nursing cubs tested. The finding of T. gondii antibodies in only 3 of 66 cubs, and higher antibody titers in their respective sows indicates that the colostrally-acquired antibodies wane to undetectable levels by 8-10 weeks, while the cubs are still in the den. The results indicate that there is no transplacental transmission of T. gondii, that antibodies acquired from colostrum are largely undetectable by the time cubs emerge from the den, and nearly that 50% of bears acquire infection postnatally by 10 months of age. This is the first report of disappearance of transcolostral antibodies of any infection in bears. (C) 2016 Published by Elsevier B.V. C1 [Dubey, Jitender P.; Verma, Shiv K.; Hill, Dolores E.; Cerqueira-Cezar, Camila K.; Kwok, Oliver C. H.; Calero-Bernal, Rafael] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Brown, Justin; Ternent, Mark] Penn Game Commiss, 2001 Elmerton Ave, Harrisburg, PA 17110 USA. [Humphreys, Jan G.] Indiana Univ Penn, Dept Biol, Indiana, PA 15705 USA. RP Dubey, JP (reprint author), USDA, BARC, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 30 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD OCT 15 PY 2016 VL 229 BP 76 EP 80 DI 10.1016/j.vetpar.2016.09.013 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA ED0RI UT WOS:000388549200013 PM 27809983 ER PT J AU Tuo, WB Li, L Lv, YJ Carrillo, J Brown, D Davis, WC Song, JZ Zarlenga, D Xiao, ZG AF Tuo, Wenbin Li, Lei Lv, Yingjun Carrillo, Jose Brown, Dedra Davis, William C. Song, Jiuzhou Zarlenga, Dante Xiao, Zhengguo TI Abomasal mucosal immune responses of cattle with limited or continuous exposure to pasture-borne gastrointestinal nematode parasite infection SO VETERINARY PARASITOLOGY LA English DT Article DE No pasture exposure (NPE); Limited pasture exposure (LPE); Continuous pasture exposure (CPE); Cattle; Gastrointestinal nematode parasite; Ostertagia ostertagi; B cells; T cells ID OSTERTAGIA-OSTERTAGI INFECTION; FATTY-ACID PROFILES; GRAIN-FED CATTLE; RED MEAT; FED BEEF; QUALITY; CALVES; GRASS; RESISTANCE; RUMINANTS AB It has been well documented that cattle raised on pasture are slow in weight gain when compared to those fed with grain. Inflammation in the digestive system commonly caused by pasture-transmitted gastrointestinal (GI) nematode parasites that could negatively impact feed conversion has never been compared in cattle raised with no pasture exposure (NPE, uninfected), limited pasture exposure (LPE, exposure until weaning), or continuous pasture exposure (CPE, life time exposure). In the present study, the abomasal mucosa] immune responses and inflammation of LPE and CPE cattle were investigated. Our results indicate that CPE cattle displayed inflamed abomasa with enlarged draining lymph nodes, the presence of Ostertagia ostertagi larvae and higher levels of Ostertagia-specific antibodies in circulation. The level of B cells was elevated in the abomasal mucosa in the presence (nodular) or absence (non-nodular) of Ostertagia-specific pathology, where B cells were 4-fold higher in the nodular mucosa. Foxp3(+) CD4T cells were also noticeably elevated in both the abomasal mucosa and blood, but were only slightly higher in non-nodular mucosa than in the nodular mucosa of CPE animals. In contrast, LPE animals presented no enlargement of abomasal draining lymph nodes and exhibited little to no immune cell infiltration in the abomasal mucosa. Further, CPE animals had higher numbers of mucosal mast cells when compared to LPE animals, though mucosal mast cells were high in all animals. Overall, CPE cattle displayed significantly higher levels of inflammation and pathology in their abomasa and may explain in part slowed weight gain relative to LPE animals. The results of this study emphasize the need for GI nematode parasite control in CPE animals and development and application of vaccines which are compatible with the organic cattle production system. Published by Elsevier B.V. C1 [Tuo, Wenbin; Brown, Dedra; Zarlenga, Dante] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Li, Lei; Lv, Yingjun; Carrillo, Jose; Song, Jiuzhou; Xiao, Zhengguo] Univ Maryland, Dept Avian & Anim Sci, College Pk, MD 20742 USA. [Davis, William C.] Washington State Univ, Dept Vet Microbiol & Pathobiol, Pullman, WA 99164 USA. [Brown, Dedra] Texas A&M Univ, Dept Anim Rangeland & Wildlife Sci, 700 Univ Blvd, Kingsville, TX 78363 USA. RP Tuo, WB (reprint author), USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA.; Xiao, ZG (reprint author), Univ Maryland, Dept Avian & Anim Sci, College Pk, MD 20742 USA. EM Wenbin.tuo@ars.usda.gov; xiao0028@umd.edu FU USDA/ARS; USDA NIFA [GRANT11885997]; University of Maryland; STEP UP/USDA Project of the Texas A&M University System FX The authors are grateful to Mr. Eliseo Miramontes and Ms. Marsha Campbell of USDA/ARS for excellent technical assistance. Research was supported by funds from USDA/ARS (WT, DZ), USDA NIFA GRANT11885997 (ZX), and USDA/ARS Specific Cooperative Agreement with University of Maryland (WT, ZX). Ms. Dedra Brown was a summer student and supported by the STEP UP/USDA Project of the Texas A&M University System. Authors are grateful to the inspiring discussions with and/or critical reviews by Drs. Raymond Fetterer and Benjamin Rosenthal of APDL/BARC, USDA/ARS. NR 42 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD OCT 15 PY 2016 VL 229 BP 118 EP 125 DI 10.1016/j.vetpar.2016.10.005 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA ED0RI UT WOS:000388549200020 PM 27809966 ER PT J AU Payabyab, EC Balasubramaniam, S Edgerly, M Velarde, M Merino, MJ Venkatesan, AM Leuva, H Litman, T Bates, SE Fojo, T AF Payabyab, Eden C. Balasubramaniam, Sanjeeve Edgerly, Maureen Velarde, Margarita Merino, Maria J. Venkatesan, Aradhana M. Leuva, Harshraj Litman, Thomas Bates, Susan E. Fojo, Tito TI Adrenocortical Cancer: A Molecularly Complex Disease Where Surgery Matters SO CLINICAL CANCER RESEARCH LA English DT Article ID ADRENAL-CORTICAL CARCINOMA; LONG-TERM SURVIVAL; LAPAROSCOPIC ADRENALECTOMY; GENOMIC CHARACTERIZATION; ONCOLOGIC OUTCOMES; ADJUVANT MITOTANE; PHASE-II; STAGE-I; THERAPY; TUMORS AB The development of new therapies has lagged behind for rare cancers without defined therapeutic targets. Adrenocortical cancer is no exception. Mitotane, an older agent considered "adrenolytic," is used both to control symptoms in advanced disease and as adjuvant therapy after surgical resection. Molecular characterization of adrenocortical cancer has deepened our understanding of this genetically complex disease while identifying subgroups whose importance remains to be determined. Unfortunately, such studies have yet to demonstrate a therapeutic target for drug development, and to date, no targeted therapy has achieved meaningful outcomes. Consequently, first-line therapy for metastatic disease remains a combination regimen of etoposide, doxorubicin, and cisplatinum established in a randomized clinical trial. In addition to evaluating recent studies in adrenocortical cancer, we raise one critical clinical issue-the risk of peritoneal dissemination following laparoscopic resection of adrenocortical cancer. In a retrospective case series of 267 patients referred to the NCI for the treatment of recurrent or advanced adrenocortical cancer, we found extensive peritoneal dissemination in 25 of the 45 patients (55.6%) who had undergone laparoscopic resection, compared with only 7 of the 222 patients (3%) who had undergone an open resection (P < 0.0001). Although this has been debated in the literature, our data argue for an end to laparoscopic resection of adrenocortical cancers to avoid peritoneal dissemination, a complication of laparoscopy that is uniformly fatal. (C) 2016 AACR. C1 [Payabyab, Eden C.] NCI, Surg Branch, NIH, Bldg 10, Bethesda, MD 20892 USA. [Payabyab, Eden C.] NCI, Thorac & GI Oncol Branch, NIH, Bethesda, MD 20892 USA. [Balasubramaniam, Sanjeeve] USDA, Div Oncol Prod 1, OHOP, CDER, Silver Spring, MD USA. [Edgerly, Maureen; Velarde, Margarita] NCI, Med Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. [Merino, Maria J.] NCI, Pathol Lab, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA. [Venkatesan, Aradhana M.] Univ Texas MD Anderson Canc Ctr, Dept Diagnost Radiol, Div Diagnost Imaging, Houston, TX 77030 USA. [Leuva, Harshraj; Bates, Susan E.; Fojo, Tito] James J Peters Vet Adm Med Ctr, Bronx, NY USA. [Litman, Thomas] Univ Copenhagen, Med Biostat, Copenhagen, Denmark. [Bates, Susan E.; Fojo, Tito] Columbia Univ, Dept Med, Med Ctr, Div Med Oncol, New York, NY 10032 USA. RP Fojo, T (reprint author), Columbia Univ, Dept Med, Med Ctr, Div Med Oncol, New York, NY 10032 USA.; Fojo, T (reprint author), James J Peters VA Med Ctr, Bronx, NY 10468 USA. EM atf2116@cumc.columbia.edu OI Balasubramaniam, Sanjeeve/0000-0002-0643-2117 NR 57 TC 2 Z9 2 U1 2 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1078-0432 EI 1557-3265 J9 CLIN CANCER RES JI Clin. Cancer Res. PD OCT 15 PY 2016 VL 22 IS 20 BP 4989 EP 5000 DI 10.1158/1078-0432.CCR-16-1570 PG 12 WC Oncology SC Oncology GA DZ1WP UT WOS:000385632700007 PM 27742785 ER PT J AU Schell, CJ Young, JK Lonsdorf, EV Mateo, JM Santymire, RM AF Schell, Christopher J. Young, Julie K. Lonsdorf, Elizabeth V. Mateo, Jill M. Santymire, Rachel M. TI Olfactory attractants and parity affect prenatal androgens and territoriality of coyote breeding pairs SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Androgens; Coyotes; Glucocorticoids; Parity; Personality; Repeatability ID AFRICAN WILD DOGS; CONSISTENT INDIVIDUAL-DIFFERENCES; FEMALE ZEBRA FINCHES; COTTON-TOP TAMARINS; MALE HOUSE SPARROWS; CANIS-LATRANS; GLUCOCORTICOID LEVELS; TESTOSTERONE LEVELS; SOCIAL-DOMINANCE; ETHIOPIAN WOLVES AB Hormones are fundamental mediators of personality traits intimately linked with reproductive success. Hence, alterations to endocrine factors may dramatically affect individual behavior that has subsequent fitness consequences. Yet it is unclear how hormonal or behavioral traits change with environmental stressors or over multiple reproductive opportunities, particularly for biparental fauna. To simulate an environmental stressor, we exposed captive coyote (Canis latrans) pairs to novel coyote odor attractants (i.e. commercial scent lures) mid gestation to influence territorial behaviors, fecal glucocorticoid (FGMs) and fecal androgen metabolites (FAMs). In addition, we observed coyote pairs as first-time and experienced breeders to assess the influence of parity on our measures. Treatment pairs received the odors four times over a 20-day period, while control pairs received water. Odor-treated pairs scent-marked (e.g. urinated, ground scratched) and investigated odors more frequently than control pairs, and had higher FAMs when odors were provided. Pairs had higher FAMs as first-time versus experienced breeders, indicating that parity also affected androgen production during gestation. Moreover, repeatability in scent-marking behaviors corresponded with FGMs and FAMs, implying that coyote territoriality during gestation is underpinned by individually-specific hormone profiles. Our results suggest coyote androgens during gestation are sensitive to conspecific olfactory stimuli and prior breeding experience. Consequently, fluctuations in social or other environmental stimuli as well as increasing parity may acutely affect coyote traits essential to reproductive success. (C) 2016 Elsevier Inc All rights reserved. C1 [Schell, Christopher J.; Mateo, Jill M.; Santymire, Rachel M.] Univ Chicago, Comm Evolutionary Biol, 1025 E 57th St,Culver Hall 402, Chicago, IL 60637 USA. [Young, Julie K.] Utah State Univ, USDA WS NWRC, Predator Res Facil, Dept Wildland Resources, USU BNR 163, Logan, UT 84322 USA. [Lonsdorf, Elizabeth V.] Franklin & Marshall Coll, Dept Psychol, POB 3003, Lancaster, PA 17603 USA. [Santymire, Rachel M.] Lincoln Pk Zoo, Dept Conservat & Sci, 2001 N Clark St, Chicago, IL 60614 USA. RP Schell, CJ (reprint author), USDA APHIS WS NWRC, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM cjschell@colostate.edu OI Schell, Christopher/0000-0002-2073-9852 FU University of Chicago Hinds Fund; GAANN Fellowship; United Negro College Fund (UNCF)-Merck Fellowship; National Science Foundation (NSF); U.S. Department of Agriculture Wildlife Services-NWRC FX We would like to thank B. Patterson and T. Price for extensive and valuable feedback on this manuscript, as well as members of the Mateo and Santymire laboratories. This study would not have been possible without the input, time, and animal expertise of S. Brummer, J. Schultz, M. Davis, E. Stevenson, and other staff at the Wildlife Services National Wildlife Research Center's Predator Research Facility in Millville, UT, USA. Special thanks to D. Schell, B. Flack, and other volunteers for providing data collection assistance, behavioral observations, and laboratory analyses. Additional thanks to the University of Chicago Hinds Fund and GAANN Fellowship, the United Negro College Fund (UNCF)-Merck Fellowship, the National Science Foundation (NSF), and U.S. Department of Agriculture Wildlife Services-NWRC as funding sources for this endeavor. This work was performed with the approval of the Institutional Animal Care and Use Committees (IACUC) at the University of Chicago (protocol no. 72184), the NWRC (protocol no. QA-1818), and the Lincoln Park Zoo Research Committee. Mention of companies or commercial products does not imply recommendation or endorsement by USDA over others not mentioned. USDA neither guarantees nor warrants the standard of any product mentioned. Product names are mentioned solely to report factually on available data and to provide specific information. NR 114 TC 0 Z9 0 U1 17 U2 17 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD OCT 15 PY 2016 VL 165 BP 43 EP 54 DI 10.1016/j.physbeh.2016.06.038 PG 12 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA DY0LS UT WOS:000384788900007 PM 27378509 ER PT J AU Krishnan, S Tryon, RR Horn, WF Welch, L Keim, NL AF Krishnan, Sridevi Tryon, Rebecca R. Horn, William F. Welch, Lucas Keim, Nancy L. TI Estradiol, SHBG and leptin interplay with food craving and intake across the menstrual cycle SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Leptin; Estradiol; Food intake; Sex hormones; Craving ID HORMONE-BINDING GLOBULIN; BODY-WEIGHT; ENERGY-BALANCE; FEMALE RATS; WOMEN; ESTROGEN; PREMENOPAUSAL; PROGESTERONE; DETERMINANTS; PREFERENCES AB Objective: To understand the association between ovarian hormones, non-acute satiety hormones and craving calorie dense foods in the luteal phase. Methods: 17 premenopausal women, mean age 23.2 y, mean BMI 22.4 kg/m(2) with regular menstrual cycles were studied during late follicular (FP) and luteal phases (LP). Estradiol, progesterone, DHEAS, SHBG, insulin and leptin, were measured in fasting samples. The validated' Food Craving Inventory was used to record the types of foods volunteers habitually ate - rich in fat, carbohydrate or sweet taste, as well as craved during the LP of their menstrual cycle. Results: Estradiol was inversely associated with leptin in FP (r = 0.62, p = 0.01). Leptin was inversely associated with habitual intake of sweet foods, in both phases (FP: r = 0.64, p = 0.01; LP: r = 0.63, p = 0.01). SHBG in LP was positively associated with craving sweet and carbohydrate rich foods. Hierarchical cluster analysis revealed two groups of women, one with high estradiol, high estradiol/leptin ratio, high sweet and carbohydrate cravings (p < 0.05); the other group had lower estradiol, lower estradiol/leptin ratio, and reported less craving. Conclusions: The estradiol-leptin axis may be a determinant of luteal phase craving and habitual food intake in menstruating women. Published by Elsevier Inc. C1 [Krishnan, Sridevi; Tryon, Rebecca R.; Welch, Lucas; Keim, Nancy L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Horn, William F.; Keim, Nancy L.] ARS, USDA, Western Human Nutr Res Ctr, Obes & Metab Res Unit, 430 West Hlth Sci Dr, Davis, CA 95616 USA. RP Keim, NL (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. FU USDA Project [5306-51530-019-00D, 2032-51530-022-00D]; UC Davis FX This study was funded by USDA Project 5306-51530-019-00D, 2032-51530-022-00D, and Jastro Shields award from UC Davis. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 42 TC 0 Z9 0 U1 7 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD OCT 15 PY 2016 VL 165 BP 304 EP 312 DI 10.1016/j.physbeh.2016.08.010 PG 9 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA DY0LS UT WOS:000384788900039 PM 27527001 ER PT J AU McRoberts, RE Chen, Q Domke, GM Stahl, G Saarela, S Westfall, JA AF McRoberts, Ronald E. Chen, Qi Domke, Grant M. Stahl, Goran Saarela, Svetlana Westfall, James A. TI Hybrid estimators for mean aboveground carbon per unit area SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Allometric model; Uncertainty analysis; Wood density; Carbon content ID NATIONAL FOREST INVENTORY; BIOMASS EXPANSION FACTORS; LIDAR-ASSISTED ESTIMATION; INDIVIDUAL-TREE VOLUME; WOOD DENSITY; FIELD-MEASUREMENTS; MEASUREMENT ERRORS; MODEL PREDICTIONS; TROPICAL FORESTS; HEDMARK COUNTY AB Carbon accounting is at the heart of efforts to mitigate the effects of climate change. One approach for estimating population parameters for live tree stem carbon entails three primary steps: (1) construction of an individual tree, allometric carbon model, (2) application of the model to tree-level data for a probability sample of plots, and (3) use of a probability-based (design-based) estimator of mean carbon per unit area for a population of interest. Compliance with the IPCC good practice guidance requires satisfaction of two criteria, one related to minimizing bias and one related to minimizing uncertainty. For this carbon estimation procedure, the portion of uncertainty attributed to the variance of the probability based estimator of the population mean using the plot-level predictions is usually correctly estimated, but the portion attributed to the variance of the allometric model estimator is usually ignored. The result is that the total variance of the population mean estimator cannot be asserted to comply with the IPCC good practice criteria because not only is it not minimized, it is not even correctly estimated. Within the framework of what is coming to be characterized as hybrid inference, model-based inferential methods were used to estimate the variance of the tree-level allometric model estimator which was then propagated through to the variance of the probability-based estimator of mean carbon per unit area. This combined estimator, consisting of a model-based estimator used to predict a variable for a probability sample of a population followed by a probability-estimator of the population total or mean using the sample predictions, is characterized as a hybrid estimator. For this study, two probability-based estimators of the mean were considered, simple random sampling estimators and model-assisted regression estimators that used airborne laser scanning (ALS) data as auxiliary information. The variance of the allometric model estimator incorporated variances of distributions of diameter and height measurement errors, covariances of model parameter estimators, model residual variance, and variances of distributions of wood densities and carbon content proportions. The novel features of the study included the hybrid inferential framework, consideration of six sources of uncertainty including the variances of distributions of wood densities and carbon content proportions, use of ALS data with model-assisted regression estimators of the population mean, and use of confidence intervals for the population mean as the basis for comparisons rather than intermediate products such as model prediction accuracy. The primary conclusions were that the variance of the allometric model estimator was negligible or marginally negligible relative to the variance of the probability estimator when using species-specific allometric models and simple random sampling estimators, but non-negligible when using species-specific models and model-assisted regression estimators and when using a nonspecific model with either estimator. Published by Elsevier B.V. C1 [McRoberts, Ronald E.; Domke, Grant M.] US Forest Serv, Northern Res Stn, St Paul, MN USA. [Chen, Qi] Univ Hawaii Manoa, Dept Geog, Honolulu, HI 96822 USA. [Stahl, Goran; Saarela, Svetlana] Swedish Univ Agr Sci, Umea, Sweden. [Westfall, James A.] US Forest Serv, Northern Res Stn, Newtown Sq, PA USA. RP McRoberts, RE (reprint author), US Forest Serv, Northern Res Stn, St Paul, MN USA. EM rmcroberts@fs.fed.us OI Saarela, Svetlana/0000-0002-9044-7249; Domke, Grant/0000-0003-0485-0355 NR 57 TC 2 Z9 2 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 44 EP 56 DI 10.1016/j.foreco.2016.07.007 PG 13 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800005 ER PT J AU Cowan, AD Smith, JE Fitzgerald, SA AF Cowan, Ariel D. Smith, Jane E. Fitzgerald, Stephen A. TI Recovering lost ground: Effects of soil burn intensity on nutrients and ectomycorrhiza communities of ponderosa pine seedlings SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Ectomycorrhizal fungi; Fire; Soil nutrients; Ponderosa pine; Large downed wood; Burn severity ID FINE-SCALE DISTRIBUTION; COARSE WOODY DEBRIS; MAZAMA CRATER LAKE; FUNGAL COMMUNITIES; PRESCRIBED-FIRE; CENTRAL OREGON; FOREST SOILS; ENHANCED SPECIFICITY; RESISTANT PROPAGULES; MURICATA SEEDLINGS AB Fuel accumulation and climate shifts are predicted to increase the frequency of high-severity fires in ponderosa pine (Pinus ponderosa) forests of central Oregon. The combustion of fuels containing large downed wood can result in intense soil heating, alteration of soil properties, and mortality of microbes. Previous studies show ectomycorrhizal fungi (EMF) improve ponderosa seedling establishment after fire but did not compare EMF communities at different levels of soil burn intensity in a field setting. For this study, soil burn intensity effects on nutrients and EMF communities were compared at Pringle Falls Experimental Forest, La Pine, Oregon. Twelve replicate sites were used, each with three treatments: high intensity soil burn from large downed wood combustion (HB), low intensity soil burn (LB), and unburned control (UB). Temperatures lethal to fungi were detected at 0-cm, 5-cm, and 10-cm depths in HB soils and 0-cm depth in LB soils. Ponderosa pine seedlings planted post-burn were harvested after four months for EMF root tip analysis. We found: (a) greater differences in soil properties and nutrients in HB soils compared to LB and UB soils; (b) no differences in EMF richness and diversity among treatments; (c) weak differences in community composition based on relative abundance between UB and either burn treatments; and (d) EMF composition in HB and LB treatments correlated with soil carbon and organic matter contents. These results support the hypothesis that the combustion of large downed wood can alter the soil environment directly beneath it. However, an EMF community similar to LB soils recolonized HB soils within one growing season. Community results from both burn treatments suggest an increase in patchy spatial distribution of EMF. We hypothesize that quick initiation of EMF recolonization is possible depending on the size of high intensity burn patches, proximity of low and unburned soil, and survival of nearby hosts. The importance of incorporating mixed fire effects in fuel management practices will help to provide EMF refugia for ponderosa pine forest regeneration. (C) Published by Elsevier B.V. C1 [Cowan, Ariel D.] Oregon State Univ, Coll Forestry, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Smith, Jane E.] US Forest Serv, USDA, Pacific Northwest Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Fitzgerald, Stephen A.] Oregon State Univ, Dept Forest Engn Resources & Management, Coll Forestry, 280 Peavy Hall, Corvallis, OR 97331 USA. RP Smith, JE (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM jsmith01@fed.fs.us FU USDA Forest Service Pacific Northwest Research Station FX The authors would like to thank Daniel Luoma, Carol Shestak, Debbie Dumroese, Joanne Tirocke, Dan Mikowski, Loretta Ellenburg, Brett Morrissette, Mike J. Vernon, Tara Jennings, Doni McKay, Joyce Eberhart, and Rich Cronn for their assistance in methodology, training, and site setup. Thank you to Alex Enna, the Bend and Redmond fire crews for making the prescribed burn possible. Also, many thanks to Lisa Ganio, Ariel Muldoon, and Greg Brenner for their statistical advice. We greatly appreciate Lucas J. Longway, Ben Hart, Maria O. Garcia, Joseph Cagle, and Elizabeth Bowman for their help with data collection and moral support. Thank you to Chris Dunn and Melanie Jones for offering experienced advice. Additionally, we would like to thank Jeff Hatten, Daniel Luoma, Tom Horton, and anonymous reviewers for reviewing this manuscript. Lastly, we are grateful for the funding support provided by the USDA Forest Service Pacific Northwest Research Station. Mention of trade or company names does not constitute an endorsement by the US Department of Agriculture. NR 108 TC 1 Z9 1 U1 38 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 160 EP 172 DI 10.1016/j.foreco.2016.07.030 PG 13 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800015 ER PT J AU Ritchie, MW AF Ritchie, Martin W. TI Multi-scale reference conditions in an interior pine-dominated landscape in northeastern California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Stand density; Biomass; Crown area; Ponderosa pine ID FOREST STRUCTURE; RESTORATION ECOLOGY; SOUTHERN CASCADES; SPATIAL-PATTERNS; PONDEROSA; USA; FIRE; VARIABILITY; STANDS AB A plot-based census was conducted of trees >8.9 cm in breast height diameter in a 4000 ha forest in northeastern California in 1933 and 1934, prior to any harvest activity. The trees were tallied by size class and species on contiguous plots specified to be 1.01 ha in size, although some plots had a forested area less than this specification due to natural openings in the forest. In general, variability in all metrics declined as scale increased across a range from 1 ha to 244 ha, although much of this variability was in the tails; the inner quartile range appeared to be more stable, particularly so for crown area. Although metrics were derived at a smaller scale (<1 ha) from partial plots, these may be unreliable due to the study design and possible confounding factors. The landscape distribution of tree sizes was bimodal and skew positive with a maximum tree size of 189.2 cm. However locally the distribution of tree sizes was more ragged and variable in shape. Species distribution appears to have shifted in a direction away from pine dominance in the years since the census was conducted. Historically, these stands were approximately 86 percent pine by basal area, with some variation depending on slope position. The stem density was dominated by young, thrifty-mature trees of good or moderate vigor but basal area and crown area was dominated by mature or over-mature trees with moderate or poor vigor. Crown area tended to be low with 90 percent of observations between 13 and 35 percent at the 1 ha scale. Published by Elsevier B.V. C1 [Ritchie, Martin W.] US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. RP Ritchie, MW (reprint author), US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM mritchie@fs.fed.us FU U.S. Forest Service, Pacific Southwest Research Station FX This work was funded internally by the U.S. Forest Service, Pacific Southwest Research Station. NR 39 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD OCT 15 PY 2016 VL 378 BP 233 EP 243 DI 10.1016/j.foreco.2016.07.017 PG 11 WC Forestry SC Forestry GA DX5AS UT WOS:000384392800022 ER PT J AU Schlink, SN Lager, KM Brockmeier, SL Loving, CL Miller, LC Vorwald, AC Yang, HC Kehrli, ME Faaberg, KS AF Schlink, Sarah N. Lager, Kelly M. Brockmeier, Susan L. Loving, Crystal L. Miller, Laura C. Vorwald, Ann C. Yang, Han-Chun Kehrli, Marcus E., Jr. Faaberg, Kay S. TI Enhancement of innate immunity with granulocyte colony-stimulating factor did not mitigate disease in pigs infected with a highly pathogenic Chinese PRRSV strain SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE HP-PRRSV; Neutrophils; G-CSF; Swine ID RESPIRATORY SYNDROME VIRUS; SINGLE-ADMINISTRATION PEGFILGRASTIM; UNITED-STATES; MYCOPLASMA-HYOPNEUMONIAE; DAILY FILGRASTIM; DAIRY-COWS; G-CSF; EMERGENCE; NEUTROPHILS; BOVINE AB Porcine reproductive and respiratory syndrome virus (PRRSV) is responsible for one of the most economically important diseases in swine worldwide. It causes reproductive failure in sows and pneumonia in pigs that predisposes them to secondary bacterial infections. Methods to control PRRSV and/or limit secondary bacterial infections are desired to reduce the impact of this virus on animal health. Neutrophils play a major role in combatting infection; they can act as phagocytes as well as produce and release lytic enzymes that have potent antimicrobial effects leading to the destruction and clearance of bacterial pathogens. Granulocyte-colony stimulating factor (G-CSF) is a cytokine that controls the production, differentiation and function of granulocytes (including neutrophils) from the bone marrow. Recent work from our laboratory has shown that encoding porcine G-CSF in a replication-defective adenovirus (Ad5-G-CSF) and delivering a single dose to pigs induced a neutrophilia lasting more than two weeks. As secondary bacterial infection is a common occurrence following PRRSV infection, particularly following challenge with highly pathogenic (HP)-PRRSV, the aim of the current study was to evaluate the effectiveness of a single prophylactic dose of adenovirus-encoded G-CSF to mitigate secondary bacterial disease associated with HP-PRRSV infection. Administration of Ad5-G-CSF induced a significant neutrophilia as expected. However, between 1 and 2 days following HP-PRRSV challenge the number of circulating neutrophils decreased dramatically in the HP-PRRSV infected group, but not the non-infected Ad5-G-CSF group. Ad5-G-CSF administration induced monocytosis as well, which was also reduced by HP-PRRSV challenge. There was no difference in the progression of disease between the Ad5-G-CSF and Ad5-empty groups following HP-PRRSV challenge, with pneumonia and systemic bacterial infection occurring in both treatment groups. Given the impact of HP-PRRSV infection on the neutrophilia induced by the Ad5-G-CSF administration, additional studies are warranted to evaluate the timing of Ad5-G-CSF induced neutrophilia and multiple G-CSF inoculations on protection against secondary bacterial infection following PRRSV infection. Nevertheless, this study may provide insight into the pathogenesis of HP-PRRSV. Published by Elsevier B.V. C1 [Schlink, Sarah N.; Lager, Kelly M.; Brockmeier, Susan L.; Loving, Crystal L.; Miller, Laura C.; Vorwald, Ann C.; Kehrli, Marcus E., Jr.; Faaberg, Kay S.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Mailstop 2S-209,1920 Dayton Ave, Ames, IA 50010 USA. [Yang, Han-Chun] China Agr Univ, Beijing, Peoples R China. [Schlink, Sarah N.] Sinclair Res Ctr LLC, Columbia, MO USA. RP Lager, KM (reprint author), ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Mailstop 2S-209,1920 Dayton Ave, Ames, IA 50010 USA. EM kelly.lager@ars.usda.gov OI Miller, Laura/0000-0002-8946-9416 NR 37 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD OCT 15 PY 2016 VL 179 BP 70 EP 76 DI 10.1016/j.vetimm.2016.08.007 PG 7 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA DV9YO UT WOS:000383298300010 PM 27590428 ER PT J AU Hsu, YM Bytnerowicz, A Fenn, ME Percy, KE AF Hsu, Yu-Mei Bytnerowicz, Andrzej Fenn, Mark E. Percy, Kevin E. TI Atmospheric dry deposition of sulfur and nitrogen in the Athabasca Oil Sands Region, Alberta, Canada SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Passive samplers; Atmospheric dry deposition; Nitrogen; Sulfur; Multi-layer inference model; Throughfall measurement ID PASSIVE SAMPLING SYSTEM; AMBIENT NITRIC-ACID; CANOPY INTERACTIONS; MULTILAYER MODEL; FORESTS; AMMONIA; AIR; ECOSYSTEMS; POLLUTANTS; PARTICLES AB Due to the potential ecological effects on terrestrial and aquatic ecosystems from atmospheric deposition in the Athabasca Oil Sands Region (AOSR), Alberta, Canada, this study was implemented to estimate atmospheric nitrogen (N) and sulfur (S) inputs. Passive samplers were used to measure ambient concentrations of ammonia (NH3), nitrogen dioxide (NO2), nitric acid,Mitrous acid (HNO3/HONO), and sulfur dioxide (SO2) in the AOSR. Concentrations of NO2 and SO2 in winter were higher than those in summer, while seasonal differences of NH3 and HNO3, HONO showed an opposite trend, with higher values in summer. Concentrations of NH3, NO2 and SO2 were high close to the emission sources (oil sands operations and urban areas). NH3 concentrations were also elevated in the southern portion of the domain indicating possible agricultural and urban emission sources to the southwest. HNO3, an oxidation endpoint, showed wider ranges of concentrations and a larger spatial extent. Concentrations of NH3, NO2, HNO3/HONO and SO2 from passive measurements and their monthly deposition velocities calculated by a multi-layer inference model (MLM) were used to calculate dry deposition of N and S. NH3 contributed the largest fraction of deposited N across the network, ranging between 0.70-1.25 kg N ha(-1) yr(-1), HNO3/HONO deposition ranged between 0.30-0.90 kg N ha(-1) yr(-1), and NO2 deposition between 0.03-0.70 kg N ha(-1) yr(-1). During the modeled period, average dry deposition of the inorganic gaseous N species ranged between 1.03 and 2.85 kg N ha(-1) yr(-1) and SO4-S deposition ranged between 026 and 2.04 kg ha(-1) yr(-1). Comparisons with co measured ion exchange resin throughfall data (8.51 kg S ha(-1) yr(-1)) indicate that modeled dry deposition combined with measured wet deposition (137 kg S ha(-1) yr(-1)) underestimated S deposition. Gas phase NH3 (71%) and HNO3 plus NO2 (79%) dry deposition fluxes dominated the total deposition of NH4-N and NO3-N, respectively. (C) 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). C1 [Hsu, Yu-Mei; Percy, Kevin E.] Wood Buffalo Environm Assoc, 100-330 Thickwood Blvd, Ft McMurray, AB T9K 1Y1, Canada. [Bytnerowicz, Andrzej; Fenn, Mark E.] US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. RP Hsu, YM (reprint author), Wood Buffalo Environm Assoc, 100-330 Thickwood Blvd, Ft McMurray, AB T9K 1Y1, Canada. EM yhsu@wbea.org FU WBEA FX The authors thank WBEA for providing funding in support of this work. The content and opinions expressed by the authors in this study do not necessarily reflect the views of the WBEA or of the WBEA membership. The authors would also like to thank Kristofer Siriunas for creating isopleth maps and Thomas Clair, David Spink, Sunny Cho, and Carla Davidson for their valuable comments and suggestions. NR 46 TC 2 Z9 2 U1 37 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD OCT 15 PY 2016 VL 568 BP 285 EP 295 DI 10.1016/j.scitotenv.2016.05.205 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DU5NK UT WOS:000382258300033 PM 27295600 ER PT J AU Sulman, BN Roman, DT Scanlon, TM Wang, LX Novick, KA AF Sulman, Benjamin N. Roman, D. Tyler Scanlon, Todd M. Wang, Lixin Novick, Kimberly A. TI Comparing methods for partitioning a decade of carbon dioxide and water vapor fluxes in a temperate forest SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE CO2 flux; Ecohydrology; Eddy covariance; Evapotranspiration; Flux partitioning; Water use efficiency ID NET ECOSYSTEM EXCHANGE; EDDY-COVARIANCE MEASUREMENTS; NORTHERN HARDWOOD FOREST; DECIDUOUS FOREST; USE EFFICIENCY; ENERGY FLUXES; UNITED-STATES; GAS-EXCHANGE; SAP FLOW; CO2 AB The eddy covariance (EC) method is routinely used to measure net ecosystem fluxes of carbon dioxide (CO2) and evapotranspiration (ET) in terrestrial ecosystems. It is often desirable to partition CO2 flux into gross primary production (GPP) and ecosystem respiration (RE), and to partition ET into evaporation and transpiration. We applied multiple partitioning methods, including the recently-developed flux variance similarity (FVS) partitioning method, to a ten-year record of ET and CO2 fluxes measured using EC at Morgan Monroe State Forest, a temperate, deciduous forest located in south-central Indiana, USA. While the FVS method has previously been demonstrated in croplands and grasslands, this is the first evaluation of the method in a forest. CO2 fluxes were partitioned using nonlinear regressions, FVS, and sub-canopy EC measurements. ET was partitioned using FVS and sub-canopy EC measurements, and sub-canopy potential evapotranspiration was calculated as an additional constraint on forest floor evaporation. Leaf gas exchange measurements were used to parameterize a model of water use efficiency (WUE) necessary for the FVS method. Scaled leaf gas exchange measurements also provided additional independent estimates of GPP and transpiration. There was good agreement among partitioning methods for transpiration and GPP, which also agreed well with scaled leaf gas exchange measurements. There was higher variability among methods for RE and evaporation. The sub-canopy flux method yielded lower estimates of evaporation and RE than FVS and lower estimates of RE than the nonlinear regression method, likely due to the exclusion of flux sources within the canopy but above the top of the sub-canopy tower for the sub-canopy flux method. Based on a sensitivity test, FVS flux partitioning was moderately sensitive to errors in WUE values, and underestimates of WUE significantly reduced the rate at which the algorithm was able to produce a physically valid solution. FVS partitioning has unique potential for retroactive ET partitioning at EC sites, because it relies on the same continuous measurements as EC and does not require additional specialized equipment. FVS also has advantages for partitioning CO2 fluxes, since it does not rely on the mechanistic assumptions necessary for the commonly used nonlinear regression technique. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sulman, Benjamin N.; Roman, D. Tyler; Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, 702 N Walnut Grove Ave, Bloomington, IN 47405 USA. [Sulman, Benjamin N.] Indiana Univ, Dept Biol, 1001 E 3rd St, Bloomington, IN 47405 USA. [Sulman, Benjamin N.] Princeton Univ, Dept Geosci, Program Atmospher & Ocean Sci, 300 Forrestal Rd, Princeton, NJ 08544 USA. [Roman, D. Tyler] Forest Serv, USDA, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. [Scanlon, Todd M.] Univ Virginia, Dept Environm Sci, 291 McCormick Rd, Charlottesville, VA 22904 USA. [Wang, Lixin] IUPUI, Dept Earth Sci, 723 W Michigan St, Indianapolis, IN 46202 USA. RP Sulman, BN (reprint author), Princeton Univ, Dept Geosci, Program Atmospher & Ocean Sci, 300 Forrestal Rd, Princeton, NJ 08544 USA. EM bsulman@princeton.edu FU Indiana University Collaborative Research Grant Program; Ameriflux Management Project; US Department of Energy FX Special thanks to R.P. Phillips, E.R. Brzostek, S. Scott, D. Dragoni, H.P. Schmid, and S. Grimmond for contributions to the long data record at the site. This work was funded by the Indiana University Collaborative Research Grant Program and the Ameriflux Management Project (managed by Lawrence Berkeley National Laboratory with support from the US Department of Energy). Additional computing support was provided by the Indiana University Geography Department. Thanks to the two anonymous reviewers for their helpful comments and suggestions. NR 54 TC 2 Z9 2 U1 21 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD OCT 15 PY 2016 VL 226 BP 229 EP 245 DI 10.1016/j.agrformet.2016.06.002 PG 17 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DS2KK UT WOS:000380598200019 ER PT J AU Lycett, SJ Bodewes, R Pohlmann, A Banks, J Banyai, K Boni, MF Bouwstra, R Breed, AC Brown, IH Chen, HL Dan, A DeLiberto, TJ Diep, N Gilbert, M Hill, S Ip, HS Ke, CW Kida, H Killian, ML Koopmans, MP Kwon, JH Lee, DH Lee, YJ Lu, L Monne, I Pasick, J Pybus, OG Rambaut, A Robinson, TP Sakoda, Y Zohari, S Song, CS Swayne, DE Torchetti, MK Tsai, HJ Fouchier, RAM Beer, M Woolhouse, M Kuiken, T AF Lycett, Samantha J. Bodewes, Rogier Pohlmann, Anne Banks, Jill Banyai, Krisztian Boni, Maciej F. Bouwstra, Ruth Breed, Andrew C. Brown, Ian H. Chen, Hualan Dan, Adam DeLiberto, Thomas J. Diep, Nguyen Gilbert, Marius Hill, Sarah Ip, Hon S. Ke, Chang Wen Kida, Hiroshi Killian, Mary Lea Koopmans, Marion P. Kwon, Jung-Hoon Lee, Dong-Hun Lee, Youn Jeong Lu, Lu Monne, Isabella Pasick, John Pybus, Oliver G. Rambaut, Andrew Robinson, Timothy P. Sakoda, Yoshihiro Zohari, Siamak Song, Chang-Seon Swayne, David E. Torchetti, Mia Kim Tsai, Hsiang-Jung Fouchier, Ron A. M. Beer, Martin Woolhouse, Mark Kuiken, Thijs CA Global Consortium H5N8 Related TI Role for migratory wild birds in the global spread of avian influenza H5N8 SO SCIENCE LA English DT Article ID VIRUSES AB Avian influenza viruses affect both poultry production and public health. A subtype H5N8 (clade 2.3.4.4) virus, following an outbreak in poultry in South Korea in January 2014, rapidly spread worldwide in 2014-2015. Our analysis of H5N8 viral sequences, epidemiological investigations, waterfowl migration, and poultry trade showed that long-distance migratory birds can play a major role in the global spread of avian influenza viruses. Further, we found that the hemagglutinin of clade 2.3.4.4 virus was remarkably promiscuous, creating reassortants with multiple neuraminidase subtypes. Improving our understanding of the circumpolar circulation of avian influenza viruses in migratory waterfowl will help to provide early warning of threats from avian influenza to poultry, and potentially human, health. C1 [Lycett, Samantha J.] Univ Edinburgh, Roslin Inst, Roslin EH25 9RG, Midlothian, Scotland. [Bodewes, Rogier] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, NL-3584 CL Utrecht, Netherlands. [Pohlmann, Anne; Beer, Martin] Friedrich Loeffler Inst, Inst Diagnost Virol, D-17493 Greifswald, Germany. [Banks, Jill; Brown, Ian H.] Anim & Plant Hlth Agcy, Virol Dept, Woodham Lane, Addlestone KT15 3NB, Surrey, England. [Banyai, Krisztian] Hungarian Acad Sci, Agr Res Ctr, Inst Vet Med Res, H-1143 Budapest, Hungary. [Boni, Maciej F.] Univ Oxford, Nuffield Dept Med, Ctr Trop Med, Oxford OX3 7FZ, England. [Boni, Maciej F.; Diep, Nguyen] Univ Oxford, Clin Res Unit, Wellcome Trust Major Overseas Programme, Ho Chi Minh City, Vietnam. [Bouwstra, Ruth] Wageningen Univ & Res Ctr, Cent Vet Inst, Dept Virol, NL-8221 RA Lelystad, Netherlands. [Bouwstra, Ruth] Anim Hlth Serv, NL-7400 AA Deventer, Netherlands. [Breed, Andrew C.] Anim & Plant Hlth Agcy, Dept Epidemiol Sci, Woodham Lane, Addlestone KT15 3NB, Surrey, England. [Chen, Hualan] Chinese Acad Agr Sci, Harbin Vet Res Inst, Harbin 150001, Peoples R China. [Dan, Adam] Natl Food Chain Safety Off, Vet Diagnost Directorate, H-1149 Budapest, Hungary. [DeLiberto, Thomas J.] Wildlife Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA. [Gilbert, Marius] Univ Libre Bruxelles, Spatial Epidemiol Lab SpELL, B-1050 Brussels, Belgium. [Gilbert, Marius] Fonds Natl Rech Sci, B-1000 Brussels, Belgium. [Hill, Sarah; Pybus, Oliver G.] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. [Ip, Hon S.] US Geol Survey, Wildlife Dis Diagnost Labs Branch, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA. [Ke, Chang Wen] Ctr Dis Control & Prevent Guangdong Prov, Inst Microbiol, Guangzhou 511430, Guangdong, Peoples R China. [Kida, Hiroshi] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido 0010020, Japan. [Killian, Mary Lea; Torchetti, Mia Kim] Vet Serv, Natl Vet Serv Labs, USDA, Ames, IA 50010 USA. [Koopmans, Marion P.; Fouchier, Ron A. M.; Kuiken, Thijs] Erasmus Univ, Dept Virosci, Med Ctr, NL-3015 CN Rotterdam, Netherlands. [Kwon, Jung-Hoon; Song, Chang-Seon] Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 143701, South Korea. [Lee, Dong-Hun; Swayne, David E.] USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. [Lee, Youn Jeong] Anim & Plant Quarantine Agcy, Avian Dis Div, Gimcheon, South Korea. [Lu, Lu; Rambaut, Andrew; Woolhouse, Mark] Univ Edinburgh, Ctr Immun Infect & Evolut, Edinburgh EH9 3FL, Midlothian, Scotland. [Monne, Isabella] Ist Zooprofilatt Sperimentale Venezie, Res & Innovat Dept, I-1035020 Padua, Italy. [Pasick, John] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3M4, Canada. [Pasick, John] Canadian Food Inspect Agcy, Guelph, ON N1G 4S9, Canada. [Robinson, Timothy P.] Int Livestock Res Inst ILRI, Livestock Syst & Environm LSE, POB 30709, Nairobi 00100, Kenya. [Sakoda, Yoshihiro] Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 0600818, Japan. [Zohari, Siamak] Natl Vet Inst, Dept Virol Immunobiol & Parasitol, SE-75189 Uppsala, Sweden. [Tsai, Hsiang-Jung] Council Agr, Anim Hlth Res Inst, New Taipei 25158, Taiwan. RP Kuiken, T (reprint author), Erasmus Univ, Dept Virosci, Med Ctr, NL-3015 CN Rotterdam, Netherlands. EM t.kuiken@erasmusmc.nl RI Brown, Ian/E-1119-2011; Dan, Adam/G-6018-2012; OI Dan, Adam/0000-0001-7849-7721; Hill, Sarah/0000-0002-2995-2596; Lycett, Samantha/0000-0003-3159-596X; Banyai, Krisztian/0000-0002-6270-1772; Zohari, Siamak/0000-0002-0017-4233 FU European Commission [643476]; European Commission FP7 program [278433]; U.S. Geological Survey Ecosystems Mission Area; National Institutes of Health [1R01AI101028-02A1]; United Kingdom Research Council Environmental and Social Ecology of Human Infectious Diseases UrbanZoo program [G1100783/1]; Biotechnology and Biological Sciences Research Council (BBSRC) Zoonoses in Livestock in Kenya ZooLinK programs [BB/L019019/1]; CGIAR Research Programme on Agriculture for Nutrition and Health (A4NH); Canadian Food Inspection Agency; Hungarian Academy of Sciences Lendulet (Momentum) program; Wellcome Trust [093724/B/10/Z]; University of Edinburgh Chancellor's Fellowship scheme; Roslin Institute BBSRC strategic program [BB/J004227/1]; Centre of Expertise in Animal Disease Outbreaks (EPIC) FX This study was financially supported by the European Commission H2020 program under contract number 643476 (www.compare-europe.eu) (to A.P., J.B., A.B., I.B., M.P.K., A.R., R.A.M.F., M.B., M.W., and T.K.), European Commission FP7 program under contract number 278433 (PREDEMICS) (to A.R.), the U.S. Geological Survey Ecosystems Mission Area (to H.S.I.), National Institutes of Health grant number 1R01AI101028-02A1 (to M.G.), United Kingdom Research Council Environmental and Social Ecology of Human Infectious Diseases UrbanZoo program (G1100783/1), Biotechnology and Biological Sciences Research Council (BBSRC) Zoonoses in Livestock in Kenya ZooLinK (BB/L019019/1) programs (to T.P.R. and M.W.), CGIAR Research Programme on Agriculture for Nutrition and Health (A4NH) (to T.P.R.), Canadian Food Inspection Agency (to J.P.), Hungarian Academy of Sciences Lendulet (Momentum) program (to K.B.) and the Wellcome Trust (grant number 093724/B/10/Z) (to M.W. and A.R.). S.J.L. is supported by the University of Edinburgh Chancellor's Fellowship scheme, the Roslin Institute BBSRC strategic program grant (BB/J004227/1), and the Centre of Expertise in Animal Disease Outbreaks (EPIC). We gratefully acknowledge the originating laboratories, where specimens were first obtained, and the submitting laboratories, where sequence data were generated and submitted to the EpiFlu Database of the Global Initiative on Sharing All Influenza Data (GISAID), on which this research is based. All contributors of data may be contacted directly via the GISAID website (http://platform.gisaid.org). The accession numbers (GenBank, GISAID, and/or workset identification numbers) of all genetic sequences used in this study are provided in table S9 and are accessible from the website of GISAID (http://platform.gisaid.org). We acknowledge Y. Berhane and T. Hisanaga for sequencing the Canadian virus isolates and G. Koch for his technical advice on the poultry outbreaks in the Netherlands. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Any use of trade products or firm names is for descriptive purposes and does not imply endorsement by the U.S. government. NR 13 TC 2 Z9 2 U1 22 U2 22 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD OCT 14 PY 2016 VL 354 IS 6309 BP 213 EP 217 DI 10.1126/science.aaf8852 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC0UN UT WOS:000387816500041 ER PT J AU Labourel, A Crouch, LI Bras, JLA Jackson, A Rogowski, A Gray, J Yadav, MP Henrissat, B Fontes, CMGA Gilbert, HJ Najmudin, S Basle, A Cuskin, F AF Labourel, Aurore Crouch, Lucy I. Bras, Joana L. A. Jackson, Adam Rogowski, Artur Gray, Joseph Yadav, Madhav P. Henrissat, Bernard Fontes, Carlos M. G. A. Gilbert, Harry J. Najmudin, Shabir Basle, Arnaud Cuskin, Fiona TI The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article DE cellulosome; crystallography; enzyme kinetics; enzyme mechanism; glycoside hydrolase ID CARBOHYDRATE-BINDING MODULES; CLOSTRIDIUM-THERMOCELLUM; POLYSACCHARIDE RECOGNITION; SUBSTRATE-SPECIFICITY; CRYSTAL-STRUCTURES; DEGRADATION; DECONSTRUCTION; CELLULOSOMES; BIOCHEMISTRY; REFINEMENT AB The enzymatic degradation of plant cell walls is an important biological process of increasing environmental and industrial significance. Xylan, a major component of the plant cell wall, consists of a backbone of -1,4-xylose (Xylp) units that are often decorated with arabinofuranose (Araf) side chains. A large penta-modular enzyme, CtXyl5A, was shown previously to specifically target arabinoxylans. The mechanism of substrate recognition displayed by the enzyme, however, remains unclear. Here we report the crystal structure of the arabinoxylanase and the enzyme in complex with ligands. The data showed that four of the protein modules adopt a rigid structure, which stabilizes the catalytic domain. The C-terminal non-catalytic carbohydrate binding module could not be observed in the crystal structure, suggesting positional flexibility. The structure of the enzyme in complex with Xylp--1,4-Xylp--1,4-Xylp-[-1,3-Araf]--1,4-Xylp showed that the Araf decoration linked O-3 to the xylose in the active site is located in the pocket (-2* subsite) that abuts onto the catalytic center. The -2* subsite can also bind to Xylp and Arap, explaining why the enzyme can utilize xylose and arabinose as specificity determinants. Alanine substitution of Glu(68), Tyr(92), or Asn(139), which interact with arabinose and xylose side chains at the -2* subsite, abrogates catalytic activity. Distal to the active site, the xylan backbone makes limited apolar contacts with the enzyme, and the hydroxyls are solvent-exposed. This explains why CtXyl5A is capable of hydrolyzing xylans that are extensively decorated and that are recalcitrant to classic endo-xylanase attack. C1 [Labourel, Aurore; Crouch, Lucy I.; Jackson, Adam; Rogowski, Artur; Gray, Joseph; Gilbert, Harry J.; Basle, Arnaud; Cuskin, Fiona] Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England. [Bras, Joana L. A.; Fontes, Carlos M. G. A.; Najmudin, Shabir] Univ Lisbon, CIISA Fac Med Vet, Polo Univ Alto Ajuda, Ave Univ Tecn, P-1300477 Lisbon, Portugal. [Bras, Joana L. A.; Fontes, Carlos M. G. A.] NZYTech Genes & Enzymes, P-1649038 Lisbon, Portugal. [Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Henrissat, Bernard] Aix Marseille Univ, CNRS, UMR7857, Architecture & Fonct Macromol Biol, F-13288 Marseille, France. [Henrissat, Bernard] INRA, Architecture & Fonct Macromol Biol USC1408, F-13288 Marseille, France. [Henrissat, Bernard] King Abdulaziz Univ, Dept Biol Sci, Jedda 21589, Saudi Arabia. RP Basle, A; Cuskin, F (reprint author), Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England.; Najmudin, S (reprint author), Univ Lisbon, CIISA Fac Med Vet, Polo Univ Alto Ajuda, Ave Univ Tecn, P-1300477 Lisbon, Portugal. EM shabir@fmv.ulosboa.pt; arnaud.basle@ncl.uk; Fiona.cuskin@newcastle.ac.uk RI CIISA, FMV/D-2242-2016; Fac Sci, KAU, Biol Sci Dept/L-4228-2013 FU European Research Council [322820]; Biotechnology and Biological Research Council [BB/K020358/1, BB/K001949/1]; Wellcome Trust [RES/0120/7613]; Agence Nationale de la Recherche [ANR 12-BIME-0006-01]; Fundacao para a Ciencia e Tecnologia [PTDC/BIAPRO/103980/2008, PTDC/BIAMIC/5947/2014] FX This work was supported in part by European Research Council Grant 322820 (to H. J. G. and B. H.), Biotechnology and Biological Research Council Grants BB/K020358/1 and BB/K001949/1 (to H. J. G.), Wellcome Trust Grant RES/0120/7613 (to H. J. G.), Agence Nationale de la Recherche Grant ANR 12-BIME-0006-01 (to B. H.), and Fundacao para a Ciencia e Tecnologia Grants PTDC/BIAPRO/103980/2008 and PTDC/BIAMIC/5947/2014 (to C. M. G. A. F.). The authors declare that they have no conflicts of interest with the contents of this article. NR 49 TC 0 Z9 0 U1 11 U2 11 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD OCT 14 PY 2016 VL 291 IS 42 BP 22149 EP 22159 DI 10.1074/jbc.M116.743948 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EA1DL UT WOS:000386332600028 PM 27531750 ER PT J AU Menendez-Bravo, S Roulet, J Sabatini, M Comba, S Dunn, R Gramajo, H Arabolaza, A AF Menendez-Bravo, Simon Roulet, Julia Sabatini, Mart-N Comba, Santiago Dunn, Robert Gramajo, Hugo Arabolaza, Ana TI High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Escherichia coli; Wax production; Metabolic engineering; Fed-batch fermentation; Oleochemicals ID JOJOBA OIL; HETEROLOGOUS PRODUCTION; RENEWABLE RESOURCES; VEGETABLE-OILS; BASE-STOCKS; FATTY-ACIDS; WAX ESTERS; BIOSYNTHESIS; EXPRESSION; BIOFUELS AB Background: Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety of usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage of a polyketide synthase-based system from Mycobacterium tuberculosis and developed an Escherichia coli platform with the capacity to synthesize multimethyl-branched long-chain esters (MBE) with novel chemical structures. Results: With the aim to initiate the characterization of these novel waxy compounds, here, we describe the chassis optimization of the MBE producer E. coli strain for an up-scaled oil production. By carrying out systematic metabolic engineering, we improved the final titer to 138.1 +/- 5.3 mg MBE L-1 in batch cultures. Fed-batch microbial fermentation process was also optimized achieving a maximum yield of 790.2 +/- 6.9 mg MBE L-1 with a volumetric productivity of 15.8 +/- 1.1 mg MBE (L h)(-1). Purified MBE oil was subjected to various physicochemical analyses, including differential scanning calorimetry (DSC) and pressurized-differential scanning calorimetry (P-DSC) studies. Conclusions: The analysis of the pour point, DSC, and P-DSC data obtained showed that bacterial MBE possess improved cold flow properties than several plant oils and some chemically modified derivatives, while exhibiting high oxidation stability at elevated temperatures. These encouraging data indicate that the presence of multiple methyl branches in these novel esters, indeed, conferred favorable properties which are superior to those of linear esters. C1 [Menendez-Bravo, Simon; Roulet, Julia; Sabatini, Mart-N; Comba, Santiago; Gramajo, Hugo; Arabolaza, Ana] Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Consejo Nacl Invest Cient & Tecn, Microbiol Div,IBR Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe, Argentina. [Dunn, Robert] ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Arabolaza, A (reprint author), Univ Nacl Rosario, Fac Ciencias Bioquim & Farmaceut, Consejo Nacl Invest Cient & Tecn, Microbiol Div,IBR Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe, Argentina. EM arabolaza@ibr-conicet.gov.ar FU Y-TEC/ANPCyT Grant [PID 2013-0042]; ANPCyT Grant [PICT 2013-1981] FX This work was supported by Y-TEC/ANPCyT Grant PID 2013-0042 to HG. PID 2013-0042 belongs to the Industrial Biotechnology Program of YPF-Tecnologia. This work was also supported by ANPCyT Grant PICT 2013-1981 to AA. NR 49 TC 0 Z9 0 U1 5 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD OCT 14 PY 2016 VL 9 AR 215 DI 10.1186/s13068-016-0631-x PG 14 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA DZ1EA UT WOS:000385580500001 PM 27757170 ER PT J AU Brown, AMV Wasala, SK Howe, DK Peetz, AB Zasada, IA Denver, DR AF Brown, Amanda M. V. Wasala, Sulochana K. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. Denver, Dee R. TI Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts SO SCIENTIFIC REPORTS LA English DT Article ID LATERAL GENE-TRANSFER; CYTOPLASMIC INCOMPATIBILITY; MAXIMUM-LIKELIHOOD; SOFTWARE PACKAGE; ET-AL.; ENDOSYMBIONT; EVOLUTION; BACTERIA; BIOLOGY; EXPRESSION AB Wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. We sequenced and analyzed the genome of a new Wolbachia strain (wPpe) in the plant-parasitic nematode Pratylenchus penetrans. Phylogenomic analyses placed wPpe as the earliest diverging Wolbachia, suggesting two evolutionary invasions into nematodes. The next branches comprised strains in sap-feeding insects, suggesting Wolbachia may have first evolved as a nutritional mutualist. Genome size, protein content, %GC, and repetitive DNA allied wPpe with mutualistic Wolbachia, whereas gene repertoire analyses placed it between parasite (A, B) and mutualist (C, D, F) groups. Conservation of iron metabolism genes across Wolbachia suggests iron homeostasis as a potential factor in its success. This study enhances our understanding of this globally pandemic endosymbiont, highlighting genetic patterns associated with host changes. Combined with future work on this strain, these genomic data could help provide potential new targets for plant-parasitic nematode control. C1 [Brown, Amanda M. V.; Wasala, Sulochana K.; Howe, Dana K.; Denver, Dee R.] Oregon State Univ, Dept Integrat Biol, 3029 Cordley Hall, Corvallis, OR 97331 USA. [Peetz, Amy B.; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Brown, AMV (reprint author), Oregon State Univ, Dept Integrat Biol, 3029 Cordley Hall, Corvallis, OR 97331 USA. EM browaman@oregonstate.edu FU United States Department of Agriculture grant [SCA 58-5358-3-026] FX We thank Anne-Marie Girard and Mark Dasenko at the Center for Genome Research and Bioinformatics (CGRB) at Oregon State University for assistance with confocal microscopy and sequencing. We also thank Wendy Phillips at the USDA-Agricultural Research Service for valuable discussions of this work and for assistance with nematode collection. We thank John Werren and Amanda Dolen for providing positive control Wolbachia group A + B DNA. This work was supported by the United States Department of Agriculture grant SCA 58-5358-3-026. NR 87 TC 0 Z9 0 U1 27 U2 27 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 13 PY 2016 VL 6 AR 34955 DI 10.1038/srep34955 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY9OK UT WOS:000385463500001 PM 27734894 ER PT J AU Tingley, MW Ruiz-Gutierrez, V Wilkerson, RL Howell, CA Siegel, RB AF Tingley, Morgan W. Ruiz-Gutierrez, Viviana Wilkerson, Robert L. Howell, Christine A. Siegel, Rodney B. TI Pyrodiversity promotes avian diversity over the decade following forest fire SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE bird diversity; post-fire ecosystems; time since fire; mixed-severity fire; hierarchical Bayesian; occupancy ID MIXED-CONIFER FORESTS; SIERRA-NEVADA; BIODIVERSITY CONSERVATION; SPECIES-DIVERSITY; ANT COMMUNITIES; SEVERITY; WILDFIRE; REGIMES; USA; MANAGEMENT AB An emerging hypothesis in fire ecology is that pyrodiversity increases species diversity. We test whether pyrodiversity-defined as the standard deviation of fire severity-increases avian biodiversity at two spatial scales, and whether and how this relationship may change in the decade following fire. We use a dynamic Bayesian community model applied to a multi-year dataset of bird surveys at 1106 points sampled across 97 fires in montane California. Our results provide strong support for a positive relationship between pyrodiversity and bird diversity. This relationship interacts with time since fire, with pyrodiversity having a greater effect on biodiversity at 10 years post-fire than at 1 year post-fire. Immediately after fires, patches of differing burn severities hold similar bird communities, but over the ensuing decade, bird assemblages within patches of contrasting severities differentiate. When evaluated at the scale of individual fires, fires with a greater heterogeneity of burn severities hold substantially more species. High spatial heterogeneity in severity, sometimes called 'mixed-severity fire', is a natural part of wildfire regimes in western North America, but may be jeopardized by climate change and a legacy of fire suppression. Forest management that encourages mixed-severity fire may be critical for sustaining biodiversity across fire-prone landscapes. C1 [Tingley, Morgan W.] Univ Connecticut, Ecol & Evolutionary Biol, 75 North Eagleville Rd,Unit 3043, Storrs, CT 06269 USA. [Tingley, Morgan W.; Wilkerson, Robert L.; Siegel, Rodney B.] Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. [Ruiz-Gutierrez, Viviana] Cornell Lab Ornithol, 159 Sapsucker Woods Rd, Ithaca, NY 14850 USA. [Howell, Christine A.] US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. [Howell, Christine A.] US Forest Serv, USDA, Pacific Southwest Reg Stn, 800 Buchanan St, Albany, CA 94710 USA. RP Tingley, MW (reprint author), Univ Connecticut, Ecol & Evolutionary Biol, 75 North Eagleville Rd,Unit 3043, Storrs, CT 06269 USA.; Tingley, MW (reprint author), Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. EM morgan.tingley@uconn.edu FU Pacific Southwest Region of the USDA Forest Service and Environment Now FX This project was supported financially by the Pacific Southwest Region of the USDA Forest Service and Environment Now. NR 63 TC 0 Z9 0 U1 19 U2 19 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD OCT 12 PY 2016 VL 283 IS 1840 AR 20161703 DI 10.1098/rspb.2016.1703 PG 9 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA EA3GN UT WOS:000386490000014 ER PT J AU Stephens, JL Dinger, EC Alexander, JD Mohren, SR Ralph, CJ Sarr, DA AF Stephens, Jaime L. Dinger, Eric C. Alexander, John D. Mohren, Sean R. Ralph, C. John Sarr, Daniel A. TI Bird Communities and Environmental Correlates in Southern Oregon and Northern California, USA SO PLOS ONE LA English DT Article ID KLAMATH-SISKIYOU ECOREGION; UNITED-STATES; FOREST; SCALE; CONSERVATION; BIODIVERSITY; HABITAT; PRECIPITATION; BIOGEOGRAPHY; ECOSYSTEMS AB We examined avian community ecology in the Klamath Ecoregion and determined that individual bird species co-exist spatially to form 29 statistically distinguishable bird groups. We identified climate, geography, and vegetation metrics that are correlated with these 29 bird groups at three scales: Klamath Ecoregion, vegetation formation (agriculture, conifer, mixed conifer/hardwood, shrubland), and National Park Service unit. Two climate variables (breeding season mean temperature and temperature range) and one geography variable (elevation) were correlated at all scales, suggesting that for some vegetation formations and park units there is sufficient variation in climate and geography to be an important driver of bird communities, a level of variation we expected only at the broader scale. We found vegetation to be important at all scales, with coarse metrics (environmental site potential and existing vegetation formation) meaningful across all scales and structural vegetation patterns (e.g. succession, disturbance) important only at the scale of vegetation formation or park unit. Additionally, we examined how well six National Park Service units represent bird communities in the broader Klamath Ecoregion. Park units are inclusive of most bird communities with the exception of the oak woodland community; mature conifer forests are well represented, primarily associated with conifer canopy and lacking multi-layered structure. Identifying environmental factors that shape bird communities at three scales within this region is important; such insights can inform local and regional land management decisions necessary to ensure bird conservation in this globally significant region. C1 [Stephens, Jaime L.; Alexander, John D.; Ralph, C. John] Klamath Bird Observ, Ashland, OR 97520 USA. [Dinger, Eric C.; Mohren, Sean R.; Sarr, Daniel A.] Klamath Network, Natl Pk Serv, Ashland, OR USA. [Mohren, Sean R.] Crater Lake Natl Pk, Crater Lake, OR USA. [Ralph, C. John] USDA Forest Serv, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA USA. RP Stephens, JL (reprint author), Klamath Bird Observ, Ashland, OR 97520 USA. EM jlh@klamathbird.org OI Stephens, Jaime/0000-0002-4929-5263 FU United States Department of Interior National Park Service [P13AC01404] FX Funding for this paper was provided to Klamath Bird Observatory by the United States Department of Interior National Park Service through Task Agreement P13AC01404. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the opinions or policies of the U.S. Government. Mention of trade names or commercial products does not constitute their endorsement by the U.S. Government. NR 59 TC 0 Z9 0 U1 5 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 12 PY 2016 VL 11 IS 10 AR e0163906 DI 10.1371/journal.pone.0163906 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ0CQ UT WOS:000385505300033 PM 27732625 ER PT J AU Guo, DL Xi, FF Yu, YH Zhang, XY Zhang, GH Zhong, GY AF Guo, Da-Long Xi, Fei-Fei Yu, Yi-He Zhang, Xiao-Yu Zhang, Guo-Hai Zhong, Gan-Yuan TI Comparative RNA-Seq profiling of berry development between table grape 'Kyoho' and its early-ripening mutant 'Fengzao' SO BMC GENOMICS LA English DT Article DE Grape; Early ripening; RNA-Seq; Bud mutant; Kyoho ID CLASS IV CHITINASE; FRUIT; GENE; IDENTIFICATION; EXPRESSION; REVEALS; CALCIUM; SYSTEM; STRESS; ROS AB Background: Early ripening is an important desirable attribute for fruit crops. 'Kyoho' is a popular table grape cultivar in many Asian countries. 'Fengzao' is a bud mutant of 'Kyoho' and ripens nearly 30 days earlier than 'Kyoho'. To identify genes controlling early fruit development and ripening in 'Fengzao', RNA-Seq profiles of the two cultivars were compared at 8 different berry developmental stages in both berry peel and flesh tissues. Methods: RNA-Seq profiling of berry development between 'Kyoho' and 'Fenzhao' were obtained using the Illumina HiSeq system and analyzed using various statistical methods. Expression patterns of several selected genes were validated using qRT-PCR. Results: About 447 millions of RNA-Seq sequences were generated from 40 RNA libraries covering various different berry developmental stages of 'Fengzao' and 'Kyoho'. These sequences were mapped to 23,178 and 22,982 genes in the flesh and peel tissues, respectively. While most genes in 'Fengzao' and 'Kyoho' shared similar expression patterns over different berry developmental stages, there were many genes whose expression were detected only in 'Fengzao' or 'Kyoho'. We observed 10 genes in flesh tissue and 22 genes in peel tissue were differentially expressed at FDR <= 0.05 when the mean expression of 'Fengzao' and 'Kyoho' were compared. The most noticeable one was VIT_214s0030g00950 (a superoxide dismutase gene). This ROS related gene showed lower expression levels in 'Fengzao' than 'Kyoho' in both peel and flesh tissues across various berry developmental stages with the only exception at veraison. VIT_200s0238g00060 (TMV resistance protein n-like) and VIT_213s0067g01100 (disease resistance protein at3g14460-like) were the two other noticeable genes which were found differentially expressed between the two cultivars in both peel and flesh tissues. GO functional category and KEGG enrichment analysis of DEGs indicated that gene activities related to stress and ROS were altered between the two cultivars in both flesh and peel tissues. Several differentially expressed genes of interest were successfully validated using qRT-PCR. Conclusions: Comparative profiling analysis revealed a few dozens of genes which were differentially expressed in the developing berries of 'Kyoho' and its early ripening mutant 'Fengzao'. Further analysis of these differentially expressed genes suggested that gene activities related to ROS and pathogenesis were likely involved in contributing to the early ripening in 'Fengzao'. C1 [Guo, Da-Long; Xi, Fei-Fei; Yu, Yi-He; Zhang, Xiao-Yu; Zhang, Guo-Hai] Henan Univ Sci & Technol, Coll Forestry, Luoyang 471003, Henan Province, Peoples R China. [Guo, Da-Long; Zhong, Gan-Yuan] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Zhong, GY (reprint author), USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. EM ganyuan.zhong@ars.usda.gov OI Guo, Da-Long/0000-0001-5379-5255 FU Natural Science Foundation of China (NSFC) [31372026, 31672106]; Plan for Scientific Innovation Talent of Henan Province [164100510006]; Program for Science&Technology Innovation Talents in the University of Henan Province [13HASTIT004]; Innovative Research Team in Henan University of Science & Technology, China [2015TTD003] FX This work was supported by Natural Science Foundation of China (NSFC: 31372026, 31672106), Plan for Scientific Innovation Talent of Henan Province (164100510006), Program for Science&Technology Innovation Talents in the University of Henan Province (13HASTIT004) and Innovative Research Team in Henan University of Science & Technology (2015TTD003), China. The RNA-Seq work was carried out at the USDA-Agricultural Research Service (ARS), Grape Genetics research Unit, Geneva, New York, USA. NR 59 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 795 DI 10.1186/s12864-016-3051-1 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400003 PM 27729006 ER PT J AU Leisso, RS Gapper, NE Mattheis, JP Sullivan, NL Watkins, CB Giovannoni, JJ Schaffer, RJ Johnston, JW Hanrahan, I Hertog, MLATM Nicolai, BM Rudell, DR AF Leisso, Rachel S. Gapper, Nigel E. Mattheis, James P. Sullivan, Nathanael L. Watkins, Christopher B. Giovannoni, James J. Schaffer, Robert J. Johnston, Jason W. Hanrahan, Ines Hertog, Maarten L. A. T. M. Nicolai, Bart M. Rudell, David R. TI Gene expression and metabolism preceding soft scald, a chilling injury of 'Honeycrisp' apple fruit SO BMC GENOMICS LA English DT Article DE Malus x domestica Borkh; Apples; Transcriptomics; Metabolomics; Chilling injury; Soft scald ID ALPHA-FARNESENE SYNTHASE; X DOMESTICA BORKH.; SUPERFICIAL SCALD; ISOPRENOID BIOSYNTHESIS; ANTIOXIDANT TREATMENT; CONTROLLED-ATMOSPHERE; STORAGE-TEMPERATURE; FUNCTIONAL-DISORDER; PLASTIDIAL PATHWAYS; OXIDATION-PRODUCTS AB Background: 'Honeycrisp' is an apple cultivar that is susceptible to soft scald, a chilling injury expressed as necrotic patches on the peel. Improved understanding of metabolism associated with the disorder would improve our understanding of soft scald and contribute to developing more effective management strategies for apple storage. It was expected that specific gene expression and specific metabolite levels in the peel would be linked with soft scald risk at harvest and/or specific time points during cold storage. Results: Fruit from nine 'Honeycrisp' apple orchards that would eventually develop different incidences of soft scald between 4 and 8 weeks of cold air storage were used to contrast and determine differential transcriptomic and metabolomic changes during storage. Untargeted metabolic profiling revealed changes in a number of distinct pathways preceding and concurrent with soft scald symptom development, including elevated gamma-aminobutryic acid (GABA), 1-hexanol, acylated steryl glycosides, and free rho-coumaryl acyl esters. At harvest, levels of sesquiterpenoid and triterpenoid acyl esters were relatively higher in peel of fruit that did not later develop the disorder. RNA-seq driven gene expression profiling highlighted possible involvement of genes and associated metabolic processes with soft scald development. These included elevated expression of genes involved in lipid peroxidation and phenolic metabolism in fruit with soft scald, and isoprenoid/brassinosteroid metabolism in fruit that did not develop soft scald. Expression of other stress-related genes in fruit that developed soft scald included chlorophyll catabolism, cell wall loosening, and lipid transport while superoxide dismutases were up-regulated in fruit that did not develop the disorder. Conclusions: This study delineates the sequential transcriptomic and metabolomic changes preceding soft scald symptom development. Changes were differential depending on susceptibility of fruit to the disorder and could be attributed to key stress related and mediating pathways. C1 [Leisso, Rachel S.; Mattheis, James P.; Sullivan, Nathanael L.; Rudell, David R.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Gapper, Nigel E.; Watkins, Christopher B.] Cornell Univ, Hort Sect, Sch Plant Sci, Ithaca, NY 14853 USA. [Gapper, Nigel E.] AgroFresh Solut Inc, 130 Technol Ctr Way Wenatchee, Wenatchee, WA 98801 USA. [Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Giovannoni, James J.] ARS, USDA, Plant Soil & Nutr Lab, Ithaca, NY 14853 USA. [Schaffer, Robert J.] Plant & Food Res Ltd, New Zealand Inst, Auckland, New Zealand. [Johnston, Jason W.] Plant & Food Res Ltd, New Zealand Inst, Havelock North, New Zealand. [Hanrahan, Ines] Washington Tree Fruit Res Commiss, Wenatchee, WA USA. [Hertog, Maarten L. A. T. M.; Nicolai, Bart M.] Katholieke Univ Leuven, BIOSYST MeBioS, Heverlee, Belgium. RP Rudell, DR (reprint author), ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. EM David.Rudell@ars.usda.gov FU USDA Specialty Crops Research Initiative [2010-51181-21446]; Washington Tree Fruit Research Commission; AgroFresh, Inc. FX This research was supported by the USDA Specialty Crops Research Initiative (Project number 2010-51181-21446), the Washington Tree Fruit Research Commission, and AgroFresh, Inc. NR 87 TC 0 Z9 0 U1 21 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 798 DI 10.1186/s12864-016-3019-1 PG 23 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400006 PM 27733113 ER PT J AU Lin, M Zhang, DD Liu, SB Zhang, GR Yu, JM Fritz, AK Bai, GH AF Lin, Meng Zhang, Dadong Liu, Shubing Zhang, Guorong Yu, Jianming Fritz, Allan K. Bai, Guihua TI Genome-wide association analysis on pre-harvest sprouting resistance and grain color in US winter wheat SO BMC GENOMICS LA English DT Article DE Triticum aestivum; pre-harvest sprouting resistance; grain color; genome- wide association studies ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; SEED COAT COLOR; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; GENETIC COMPONENTS; HEXAPLOID WHEAT; DORMANCY; DIVERSITY; QTL AB Background: Pre-harvest sprouting (PHS) in wheat can cause substantial reduction in grain yield and end-use quality. Grain color (GC) together with other components affect PHS resistance. Several quantitative trait loci (QTL) have been reported for PHS resistance, and two of them on chromosome 3AS (TaPHS1) and 4A have been cloned. Methods: To determine genetic architecture of PHS and GC and genetic relationships of the two traits, a genome-wide association study (GWAS) was conducted by evaluating a panel of 185 U.S. elite breeding lines and cultivars for sprouting rates of wheat spikes and GC in both greenhouse and field experiments. The panel was genotyped using the wheat 9K and 90K single nucleotide polymorphism (SNP) arrays. Results: Four QTL for GC on four chromosomes and 12 QTL for PHS resistance on 10 chromosomes were identified in at least two experiments. QTL for PHS resistance showed varied effects under different environments, and those on chromosomes 3AS, 3AL, 3B, 4AL and 7A were the more frequently identified QTL. The common QTL for GC and PHS resistance were identified on the long arms of the chromosome 3A and 3D. Conclusions: Wheat grain color is regulated by the three known genes on group 3 chromosomes and additional genes from other chromosomes. These grain color genes showed significant effects on PHS resistance in some environments. However, several other QTL that did not affect grain color also played a significant role on PHS resistance. Therefore, it is possible to breed PHS-resistant white wheat by pyramiding these non-color related QTL. C1 [Lin, Meng; Zhang, Dadong; Liu, Shubing; Zhang, Guorong; Fritz, Allan K.; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Yu, Jianming] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Bai, GH (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA.; Bai, GH (reprint author), USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP Project from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This study was partially funded by the National Research Initiative Competitive Grants CAP Project (2011-68002-30029) from the USDA National Institute of Food and Agriculture. NR 62 TC 1 Z9 1 U1 19 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 12 PY 2016 VL 17 AR 794 DI 10.1186/s12864-016-3148-6 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DZ0EN UT WOS:000385510400002 PM 27729004 ER PT J AU Cheng, TYD Shelver, WL Hong, CC McCann, SE Davis, W Zhang, YL Ambrosone, CB Smith, DJ AF Cheng, Ting-Yuan David Shelver, Weilin L. Hong, Chi-Chen McCann, Susan E. Davis, Warren Zhang, Yali Ambrosone, Christine B. Smith, David J. TI Urinary Excretion of the beta-Adrenergic Feed Additives Ractopamine and Zilpaterol in Breast and Lung Cancer Patients SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE beta(2)-adrenergic agonists; ractopamine; zilpaterol; urine; cancer; meat consumption ID TISSUE RESIDUES; HEALTH CONCERNS; AGONISTS; CLENBUTEROL; LIVESTOCK; PERFORMANCE; METABOLISM; ANIMALS; AGENT; WATER AB beta(2)-Adrenergic agonists (beta-agonists) have been legally used in the U.S. for almost two decades to increase lean muscle mass in meat animals. Despite a cardiotoxic effect after high-dose exposure, there has been limited research on human beta-agonist exposures related to meat consumption. We quantified urinary concentrations of ractopamine and zilpaterol, two FDA approved beta-agonist feed additives, and examined the extent to which the concentrations were associated with estimated usual meat intake levels. Overnight urine samples from 324 newly diagnosed breast cancer patients and spot urine samples from 46 lung cancer patients at the time of diagnosis, prior to treatment, were collected during 2006-2010 and 2014-2015, respectively. Urinary ractopamine and zilpaterol concentrations were measured by LC-MS/MS. Ractopamine and zilpaterol, respectively, were detected in 8.1% and 3.0% of the urine samples collected (n = 370). Only 1.1% (n = 4) of the urine samples had zilpaterol concentrations above the limit of quantification, with the mean value of 0.07 ng/mL in urine. The presence of detectable ractopamine and zilpaterol levels were not associated with meat consumption estimated from a food frequency questionnaire, including total meat (P = 0.13 and 0.74, respectively), total red meat (P = 0.72 and 0.74), unprocessed red meat (P = 0.74 and 0.73), processed red meat (P = 0.72 and 0.15), and poultry intake (P = 0.67 for ractopamine). Our data suggest that the amount of meat-related exposure of beta-agonists was low. C1 [Cheng, Ting-Yuan David; Hong, Chi-Chen; McCann, Susan E.; Davis, Warren; Zhang, Yali; Ambrosone, Christine B.] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Elm & Carlton St, Buffalo, NY 14263 USA. [Shelver, Weilin L.; Smith, David J.] USDA ARS, Biosci Res Lab, Fargo, ND 58103 USA. RP Cheng, TYD (reprint author), Roswell Pk Canc Inst, Dept Canc Prevent & Control, Elm & Carlton St, Buffalo, NY 14263 USA. EM david.cheng@roswellpark.org FU Susan G. Komen Breast Cancer Foundation; Breast Cancer Research Foundation [BCTR104906]; U.S. Army Medical Research and Materiel Command [DoD W81XWH0610401]; RPCI Alliance Foundation; RPCI's Cancer Center Support Grant from the National Cancer Institute [P30CA016056] FX The Women's Health after Breast Cancer (ABC) Study was supported by Susan G. Komen Breast Cancer Foundation. (BCTR104906), Breast Cancer Research Foundation, the U.S. Army Medical Research and Materiel Command (DoD W81XWH0610401), and RPCI Alliance Foundation. DBBR is supported by RPCI's Cancer Center Support Grant from the National Cancer Institute (P30CA016056). NR 45 TC 0 Z9 0 U1 4 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD OCT 12 PY 2016 VL 64 IS 40 BP 7632 EP 7639 DI 10.1021/acs.jafc.6b02723 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DY9QJ UT WOS:000385469900026 PM 27641640 ER PT J AU Reyes-Fox, M Steltzer, H LeCain, DR McMaster, GS AF Reyes-Fox, Melissa Steltzer, Heidi LeCain, Daniel R. McMaster, Gregory S. TI Five years of phenology observations from a mixed-grass prairie exposed to warming and elevated CO2 SO SCIENTIFIC DATA LA English DT Article; Data Paper ID CARBON-DIOXIDE; ATMOSPHERIC CO2; GLOBAL CHANGE; ENRICHMENT; TEMPERATURE; RESPONSES; ECOSYSTEM; GROWTH AB Atmospheric CO2 concentrations have been steadily increasing since the Industrial Era and contribute to concurrent increases in global temperatures. Many observational studies suggest climate warming alone contributes to a longer growing season. To determine the relative effect of warming on plant phenology, we investigated the individual and joint effects of warming and CO2 enrichment on a mixed-grass prairie plant community by following the development of six common grassland species and recording four major life history events. Our data support that, in a semi-arid system, while warming advances leaf emergence and flower production, it also expedites seed maturation and senescence at the species level. However, the additive effect can be an overall lengthening of the growing and reproductive seasons since CO2 enrichment, particularly when combined with warming, contributed to a longer growing season by delaying plant maturation and senescence. Fostering synthesis across multiple phenology datasets and identifying key factors affecting plant phenology will be vital for understanding regional plant community responses to climate change. C1 [Reyes-Fox, Melissa] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. [Reyes-Fox, Melissa; McMaster, Gregory S.] Northern Plains Area, Ft Collins, CO 80526 USA. [Steltzer, Heidi] Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. [LeCain, Daniel R.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [McMaster, Gregory S.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. RP Reyes-Fox, M (reprint author), USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA.; Reyes-Fox, M (reprint author), Northern Plains Area, Ft Collins, CO 80526 USA.; Steltzer, H (reprint author), Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. EM melissa.reyes@ars.usda.gov; steltzer_h@fortlewis.edu NR 26 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-4463 J9 SCI DATA JI Sci. Data PD OCT 11 PY 2016 VL 3 AR UNSP 160088 DI 10.1038/sdata.2016.88 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EF3ON UT WOS:000390234100002 PM 27727235 ER PT J AU Belamkar, V Farmer, AD Weeks, NT Kalberer, SR Blackmon, WJ Cannon, SB AF Belamkar, Vikas Farmer, Andrew D. Weeks, Nathan T. Kalberer, Scott R. Blackmon, William J. Cannon, Steven B. TI Genomics-assisted characterization of a breeding collection of Apios americana, an edible tuberous legume SO SCIENTIFIC REPORTS LA English DT Article ID REDUCED REPRESENTATION LIBRARIES; SINGLE NUCLEOTIDE POLYMORPHISM; THROUGHPUT SNP DISCOVERY; ASSOCIATION; GENERATION; SEQUENCE; QUINOA; TUBERIZATION; LEGUMINOSAE; EXPRESSION AB For species with potential as new crops, rapid improvement may be facilitated by new genomic methods. Apios (Apios americana Medik.), once a staple food source of Native American Indians, produces protein-rich tubers, tolerates a wide range of soils, and symbiotically fixes nitrogen. We report the first high-quality de novo transcriptome assembly, an expression atlas, and a set of 58,154 SNP and 39,609 gene expression markers (GEMs) for characterization of a breeding collection. Both SNPs and GEMs identify six genotypic clusters in the collection. Transcripts mapped to the Phaseolus vulgaris genome-another phaseoloid legume with the same chromosome number-provide provisional genetic locations for 46,852 SNPs. Linkage disequilibrium decays within 10 kb (based on the provisional genetic locations), consistent with outcrossing reproduction. SNPs and GEMs identify more than 21 marker-trait associations for at least 11 traits. This study demonstrates a holistic approach for mining plant collections to accelerate crop improvement. C1 [Belamkar, Vikas] Iowa State Univ, Interdept Genet, Ames, IA 50011 USA. [Belamkar, Vikas; Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Farmer, Andrew D.] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Weeks, Nathan T.; Kalberer, Scott R.; Cannon, Steven B.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Blackmon, William J.] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Dept Hort, Baton Rouge, LA 70803 USA. [Belamkar, Vikas] Univ Nebraska Lincoln, Dept Agron & Hort, Lincoln, NE 68583 USA. [Blackmon, William J.] 5097 Studley Rd, Mechanicsville, VA 23116 USA. RP Cannon, SB (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Cannon, SB (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM steven.cannon@ars.usda.gov NR 61 TC 0 Z9 0 U1 6 U2 6 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 10 PY 2016 VL 6 AR 34908 DI 10.1038/srep34908 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DY5NT UT WOS:000385147200001 PM 27721469 ER PT J AU Glenn, DM AF Glenn, D. M. TI Dry matter partitioning and photosynthetic response to biennial bearing and freeze damage in 'Empire' apple SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus domestica; Radiation use efficiency; Gas exchange; Fruit size; Leaf:fruit ratio ID CROP LOAD; GAS-EXCHANGE; FRUIT SIZE; LEAF PHOTOSYNTHESIS; VEGETATIVE GROWTH; DARK RESPIRATION; DELICIOUS APPLE; RETURN BLOOM; TREES; CANOPY AB Biennial cropping is typically characterized as having 'ON' and 'OFF' years in which the 'ON' year produces an excessive crop load and the 'OFF' year has a very small crop load. Frost damage during the bloom period or winter freeze damage is another means of reducing the crop load that can initiate biennial bearing in an otherwise consistent cropping system. The objectives were to determine what effect single and multiple year crop loss had on the current and subsequent year's dry matter partitioning and photosynthesis. Dry matter partitioning and whole tree photosynthesis (A) were being measured in biennial bearing 'Empire' apple trees when 2 consecutive frosts occurred following an 'OFF' year. This provided an opportunity to evaluate two cycles of conventional biennial bearing and the effect of 3 years of suppressed crop load on dry matter partitioning and whole tree photosynthesis. Frost events reduced fruit dry weight to levels equivalent to the 'OFF' years but the 'SUPPRESSED' years (1 'OFF' and 1 or 2 frost years) reduced partitioning to wood dry matter and leaf + fruit more than the 'OFF' years while increasing the fruit dry matter partitioning compared to the 'OFF' years despite similar fruit yield and crop load. The 'ON' years had the highest fruit dry weight and% dry weight partitioned to fruit and leaf + fruit. Conversely, the 'ON' year had the least leaf area index (LAI) and% dry weight partitioned to wood and leaf tissue. There were no differences in canopy area, however, the 'ON' years had a significantly reduced LAI. Radiation use efficiency RUE; Annual biomass (kg)/PAR [(MegaMoles/growing season) x canopy area (m(2))] was linearly correlated with the% dry mass partitioned to fruit and negatively correlated with LAI. For a specified LAI, the 'ON' year had a greater RUE than the 'OFF' or 'SUPPRESSED' years and there were no differences between 'OFF' and 'SUPPRESSED' years. Analysis of daily photosynthesis (A) indicated that all three treatments were significantly different. The 'ON' years had the highest mean daily A (3.8 mu mol/m(2)/s), 'SUPPRESSED' years were intermediate (3.0 mu mol/m(2)/s) and the 'OFF' years had the lowest A (2.5 mu mol/m(2)/s). It appears that in years when fruiting is suppressed more than a single year, dry matter partitioning to wood is also suppressed and a great partitioning occurs to the remaining fruit and leaf tissue. This may be a response to a continued deficit of the hormonal complex supplied by fruit as they develop. Published by Elsevier B.V. C1 [Glenn, D. M.] USDA, ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), USDA, ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov NR 37 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD OCT 10 PY 2016 VL 210 BP 1 EP 5 DI 10.1016/j.scienta.2016.06.042 PG 5 WC Horticulture SC Agriculture GA DX4YW UT WOS:000384388000001 ER PT J AU Cruz, JL Alves, AAC LeCain, DR Ellis, DD Morgan, JA AF Cruz, Jailson L. Alves, Alfredo A. C. LeCain, Daniel R. Ellis, David D. Morgan, Jack A. TI Elevated CO2 concentrations alleviate the inhibitory effect of drought on physiology and growth of cassava plants SO SCIENTIA HORTICULTURAE LA English DT Article DE Water deficit; Gas exchange; Water-use efficiency; Transpiration; Biomass ID ATMOSPHERIC CARBON-DIOXIDE; WATER-USE EFFICIENCY; FOREST FACE SITES; CLIMATE-CHANGE; GAS-EXCHANGE; PHOTOSYNTHETIC LIMITATIONS; MEDITERRANEAN PLANTS; MANIHOT-ESCULENTA; TEMPERATE FOREST; FOOD SECURITY AB Due to the rise in anthropogenic greenhouse gas concentrations, the earth's climate is expected to change, with precipitation being reduced in some areas resulting in growth-limiting drought and, as a consequence, reduced plant productivity. We investigated the physiological and growth responses of cassava (Manihot esculenta Crantz) to approximate present-day ambient (390 mu L L-1) and elevated (750 mu L L-1) atmospheric CO2 concentrations under well-watered and water deficit conditions, aiming at understanding how cassava would face those problems. Water deficits led to reductions in the Leaf Elongation Rate of plants grown at ambient as well as CO2-enriched concentrations. However, plants grown at 750 mu L L-1 of CO2 maintained leaf growth two days longer than plants grown at 390 mu L L-1. Three Days After Withholding Water (DAWW), photosynthesis and stomatal conductance were-reduced in plants grown under ambient CO2, while in plants under an elevated CO2 concentration, these physiological functions remained similar to that of control plants grown under good water availability. Five DAWW plants grown with 750 mu L L-1 continued to have enhanced gas exchange compared with plants grown under 390 mu L L-1. Under drought stress, the instantaneous transpiration efficiency was always greatest for plants grown under elevated CO2, The positive response of elevated CO2 levels on total dry mass was 61% in the water-stressed plants and only 20% for the plants grown under good water availability. Stomatal limitation was an important factor reducing CO2 assimilation in cassava growing under drought conditions. 2016 Elsevier B.V. All rights reserved. C1 [Cruz, Jailson L.; Alves, Alfredo A. C.] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. [LeCain, Daniel R.; Morgan, Jack A.] USDA ARS, Crops Res Lab, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Ellis, David D.] Int Potato Ctr, Ave La Molina 1895, Lima, Peru. RP Cruz, JL (reprint author), Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. EM jailson.cruz@embrapa.br FU United States Department of Agriculture/USDA-ARS; Brazilian Agricultural Research Corporation (Embrapa) FX This research was partially supported by United States Department of Agriculture/USDA-ARS and Brazilian Agricultural Research Corporation (Embrapa). The authors gratefully acknowledge Deborah P. Cruz and Maria Claudia Pelacani for kind help in growing plants. Also, thanks to Leonardo Zebral Rodrigues by providing in vitro plantlets. NR 62 TC 0 Z9 0 U1 64 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD OCT 10 PY 2016 VL 210 BP 122 EP 129 DI 10.1016/j.scienta.2016.07.012 PG 8 WC Horticulture SC Agriculture GA DX4YW UT WOS:000384388000015 ER PT J AU Zhou, Y Xu, LY Bickhart, DM Hay, EA Schroeder, SG Connor, EE Alexander, LJ Sonstegard, TS Van Tassell, CP Chen, H Liu, GE AF Zhou, Yang Xu, Lingyang Bickhart, Derek M. Hay, El Hamidi abdel Schroeder, Steven G. Connor, Erin E. Alexander, Leeson J. Sonstegard, Tad S. Van Tassell, Curtis P. Chen, Hong Liu, George E. TI Reduced representation bisulphite sequencing of ten bovine somatic tissues reveals DNA methylation patterns and their impacts on gene expression SO BMC GENOMICS LA English DT Article DE Cattle genome; Somatic tissue; DNA methylation; RRBS (reduced representation bisulphite sequencing) ID GENOME-WIDE ANALYSIS; GERM-CELL DEVELOPMENT; EMBRYONIC STEM-CELLS; NON-CPG METHYLATION; ARABIDOPSIS; SEQ; TRANSCRIPTION; ANNOTATION; EVOLUTION; PROFILES AB Background: As a major epigenetic component, DNA methylation plays important functions in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in economically important animals like cattle. Results: Using reduced representation bisulphite sequencing (RRBS), we obtained single-base-resolution maps of bovine DNA methylation from ten somatic tissues. In total, we evaluated 1,868,049 cytosines in CG-enriched regions. While we found slightly low methylation levels (29.87 to 38.06 %) in cattle, the methylation contexts (CGs and non-CGs) of cattle showed similar methylation patterns to other species. Non-CG methylation was detected but methylation levels in somatic tissues were significantly lower than in pluripotent cells. To study the potential function of the methylation, we detected 10,794 differentially methylated cytosines (DMCs) and 836 differentially methylated CG islands (DMIs). Further analyses in the same tissues revealed many DMCs (including non-CGs) and DMIs, which were highly correlated with the expression of genes involved in tissue development. Conclusions: In summary, our study provides a baseline dataset and essential information for DNA methylation profiles of cattle. C1 [Zhou, Yang; Chen, Hong] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China. [Zhou, Yang; Bickhart, Derek M.; Schroeder, Steven G.; Connor, Erin E.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, Anim Genom & Improvement Lab, BARC, Bldg 306,Room 111,BARC East, Beltsville, MD 20705 USA. [Xu, Lingyang] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China. [Hay, El Hamidi abdel; Alexander, Leeson J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Sonstegard, Tad S.] Recombinetics Inc, St Paul, MN 55108 USA. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China.; Liu, GE (reprint author), USDA ARS, Anim Genom & Improvement Lab, BARC, Bldg 306,Room 111,BARC East, Beltsville, MD 20705 USA. EM chenhong1212@263.net; George.Liu@ars.usda.gov FU AFRI grant from the USDA National Institute of Food and Agriculture (NIFA) [2011-67015-30183, 2013-67015-20951] FX This work was supported in part by AFRI grant numbers 2011-67015-30183 and 2013-67015-20951 from the USDA National Institute of Food and Agriculture (NIFA) Animal Genome and Reproduction Programs. NR 59 TC 0 Z9 0 U1 2 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD OCT 6 PY 2016 VL 17 AR 779 DI 10.1186/s12864-016-3116-1 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DY3HW UT WOS:000384982700001 PM 27716143 ER PT J AU Abdelfattah, A Wisniewski, M Droby, S Schena, L AF Abdelfattah, Ahmed Wisniewski, Michael Droby, Samir Schena, Leonardo TI Spatial and compositional variation in the fungal communities of organic and conventionally grown apple fruit at the consumer point-of-purchase SO HORTICULTURE RESEARCH LA English DT Article ID SCANNING-ELECTRON-MICROSCOPY; CORE ROT; METSCHNIKOWIA-PULCHERRIMA; ACREMONIUM-ALTERNATUM; POPULATION-DYNAMICS; ENDOPHYTIC FUNGUS; PENICILLIUM SPP.; TABLE GRAPES; BROWN SPOT; MOLDY-CORE AB The fungal diversity in harvested apples from organic or conventional management practices was analyzed in different fruit locations (stem end, calyx end, peel, and wounded flesh) shortly after fruit purchase (T1) and after 2 weeks of storage (T5). A total of 5,760,162 high-quality fungal sequences were recovered and assigned to 8,504 Operational Taxonomic Units. Members of the phylum Ascomycota were dominant in all samples and accounted for 91.6% of the total number of detected sequences. This was followed by Basidiomycota (8%), Chytridiomycota (0.1%), and unidentified fungi (0.3%). Alpha and beta diversity analyses revealed the presence of significantly different fungal populations in the investigated fruit parts. Among detected fungi, the genus Penicillium prevailed in the peel and in the wounded flesh while Alternaria spp. prevailed in the calyx and stem end samples that included apple core tissues. Several taxonomic units that appear to be closely related to pathogenic fungi associated with secondary human infections were present in peel and wounds. Moreover, significantly different populations were revealed in organic and conventional apples and this result was consistent in all investigated fruit parts (calyx end, peel, stem end, and wounded flesh). Several unique taxa were exclusively detected in organic apples suggesting that management practices may have been a contributing factor in determining the taxa present. In contrast, little differences were revealed in the two assessment times (T1 and T5). Results of the present study represent an advancement of the current knowledge on the fungal microbiota in collected fruit tissues of apple. C1 [Abdelfattah, Ahmed; Schena, Leonardo] Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Calabria, Italy. [Wisniewski, Michael] ARS, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Droby, Samir] ARO, Volcani Ctr, Dept Postharvest & Food Sci, 68 HaMccabim Rd, IL-7505101 Rishon Leziyyon, Israel. RP Schena, L (reprint author), Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89124 Calabria, Italy.; Wisniewski, M (reprint author), ARS, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Michael.Wisniewski@ars.usda.gov; lschena@unirc.it OI Abdelfattah, Ahmed/0000-0001-6090-7200 NR 91 TC 0 Z9 0 U1 11 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD OCT 5 PY 2016 VL 3 AR 16047 DI 10.1038/hortres.2016.47 PG 12 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA DY9FJ UT WOS:000385438800001 PM 27766161 ER PT J AU Girard, AL Castell-Perez, ME Bean, SR Adrianos, SL Awika, JM AF Girard, Audrey L. Castell-Perez, M. Elena Bean, Scott R. Adrianos, Sherry L. Awika, Joseph M. TI Effect of Condensed Tannin Profile on Wheat Flour Dough Rheology SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE tannin; proanthocyanidin; wheat gluten; dough rheology ID IN-VITRO STARCH; GLUTEN PROTEINS; PHENOLIC-COMPOUNDS; MIXING PROPERTIES; TORTILLA QUALITY; BREAD QUALITY; DIGESTIBILITY; PROCYANIDIN; SPECIFICITY; REDUCTION AB Proanthocyanidins (PA) cross-link proteins and could expand wheat gluten functionality; however, how the PA MW or gluten profile affect these interactions is unknown. Effect of PA MW profile (sorghum versus grape seed PA) on dough rheology of high versus low insoluble polymeric protein (IPP) wheat flour was evaluated using mixograph, large (TA.XT2i) and small (HAAKE Rheostress 6000) deformation rheometry. Sorghum PA (93% polymeric) more effectively (p < 0.05) strengthened both glutens than grape seed PA (45% polymeric), without reducing gluten extensibility. These effects were higher in low IPP (weak gluten) flour, e.g., sorghum PA doubled IPP, increased mix time by 75%, dough elasticity by 82%, and peak angle by 17 degrees versus control. Grape seed PA increased IPP by 75% and elasticity by 36%, but reduced peak angle by 15 degrees, indicating reduced mixing tolerance. Sorghum PA, but not grape seed PA, increased (p < 0.05) all above parameters in high IPP dough. Polymeric PA more effectively strengthened gluten than oligomeric PA, likely via more efficient protein cross-linking to overcome strong antioxidant effect of PA. High MW PA may be useful natural gluten strengtheners for diverse applications. C1 [Girard, Audrey L.; Awika, Joseph M.] Texas A&M Univ, Dept Soil & Crop Sci, 2474 TAMU, College Stn, TX 77843 USA. [Girard, Audrey L.; Awika, Joseph M.] Texas A&M Univ, Dept Food Sci & Nutr, College Stn, TX 77843 USA. [Castell-Perez, M. Elena] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Bean, Scott R.; Adrianos, Sherry L.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Awika, JM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, 2474 TAMU, College Stn, TX 77843 USA.; Awika, JM (reprint author), Texas A&M Univ, Dept Food Sci & Nutr, College Stn, TX 77843 USA. EM awika@tamu.edu NR 37 TC 0 Z9 0 U1 17 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD OCT 5 PY 2016 VL 64 IS 39 BP 7348 EP 7356 DI 10.1021/acs.jafc.6b02601 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DY2VZ UT WOS:000384951500006 PM 27616442 ER PT J AU Beaudart, C McCloskey, E Bruyere, O Cesari, M Rolland, Y Rizzoli, R de Carvalho, IA Thiyagarajan, JA Bautmans, I Bertiere, MC Brandi, ML Al-Daghri, NM Burlet, N Cavalier, E Cerreta, F Cherubini, A Fielding, R Gielen, E Landi, F Petermans, J Reginster, JY Visser, M Kanis, J Cooper, C AF Beaudart, Charlotte McCloskey, Eugene Bruyere, Olivier Cesari, Matteo Rolland, Yves Rizzoli, Rene de Carvalho, Islene Araujo Thiyagarajan, Jotheeswaran Amuthavalli Bautmans, Ivan Bertiere, Marie-Claude Brandi, Maria Luisa Al-Daghri, Nasser M. Burlet, Nansa Cavalier, Etienne Cerreta, Francesca Cherubini, Antonio Fielding, Roger Gielen, Evelien Landi, Francesco Petermans, Jean Reginster, Jean-Yves Visser, Marjolein Kanis, John Cooper, Cyrus TI Sarcopenia in daily practice: assessment and management SO BMC GERIATRICS LA English DT Article DE Sarcopenia; Daily practice; Assessment; Management; Tools ID X-RAY ABSORPTIOMETRY; ANTHROPOMETRIC PREDICTION EQUATIONS; BIOELECTRICAL-IMPEDANCE ANALYSIS; APPENDICULAR SKELETAL-MUSCLE; DWELLING OLDER-PEOPLE; LEAN SOFT-TISSUE; OSTEOARTHRITIS ESCEO; PHYSICAL PERFORMANCE; EUROPEAN-SOCIETY; ECONOMIC-ASPECTS AB Background: Sarcopenia is increasingly recognized as a correlate of ageing and is associated with increased likelihood of adverse outcomes including falls, fractures, frailty and mortality. Several tools have been recommended to assess muscle mass, muscle strength and physical performance in clinical trials. Whilst these tools have proven to be accurate and reliable in investigational settings, many are not easily applied to daily practice. Methods: This paper is based on literature reviews performed by members of the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO) working group on frailty and sarcopenia. Face-to-face meetings were afterwards organized for the whole group to make amendments and discuss further recommendations. Results: This paper proposes some user-friendly and inexpensive methods that can be used to assess sarcopenia in real-life settings. Healthcare providers, particularly in primary care, should consider an assessment of sarcopenia in individuals at increased risk; suggested tools for assessing risk include the Red Flag Method, the SARC-F questionnaire, the SMI method or different prediction equations. Management of sarcopenia should primarily be patient centered and involve the combination of both resistance and endurance based activity programmes with or without dietary interventions. Development of a number of pharmacological interventions is also in progress. Conclusions: Assessment of sarcopenia in individuals with risk factors, symptoms and/or conditions exposing them to the risk of disability will become particularly important in the near future. C1 [Beaudart, Charlotte; Bruyere, Olivier; Burlet, Nansa; Reginster, Jean-Yves] Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, Ave Hippocrate 13,CHU B23, B-4000 Liege, Belgium. [McCloskey, Eugene; Kanis, John] Univ Sheffield, Ctr Metab Bone Dis, Sheffield, S Yorkshire, England. [McCloskey, Eugene] MRC & Arthrit Res UK Ctr Integrated Res Musculosk, London, England. [Cesari, Matteo] Univ Hosp Toulouse, Gerontopole, Toulouse, France. [Cesari, Matteo] Univ Toulouse 3, INSERM, UMR1027, Toulouse, France. [Rolland, Yves] Univ Toulouse 3, CHU Purpan, Gerontopole Toulouse, Toulouse, France. [Rizzoli, Rene] Univ Hosp Geneva, Fac Med, Serv Bone Dis, Geneva, Switzerland. [de Carvalho, Islene Araujo; Thiyagarajan, Jotheeswaran Amuthavalli] WHO, Geneva, Switzerland. [Bautmans, Ivan] Vrije Univ Brussel, Gerontol & Frailty Ageing Res Dept, Brussels, Belgium. [Bertiere, Marie-Claude] Ctr Rech & Informat Nutritionnelles, Paris, France. [Brandi, Maria Luisa] Univ Florence, Dept Surg & Translat Med, Viale Pieraccini 6, I-59139 Florence, Italy. [Al-Daghri, Nasser M.] King Saud Univ, Coll Sci, Dept Biochem, Prince Mutaib Chair Biomarkers Osteoporosis, Riyadh 11451, Saudi Arabia. [Cavalier, Etienne] Univ Liege, CHU Sart Tilman, Dept Clin Chem, Bat B35, B-4000 Liege, Belgium. [Cerreta, Francesca] Human Med Res & Dev Support Div, Sci Advice, London, England. [Cherubini, Antonio] IRCCS INRCA, Geriatr & Geriatr Emergency Care, Ancona, Italy. [Fielding, Roger] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Gielen, Evelien] Katholieke Univ Leuven, Dept Geriatr Med, Leuven, Belgium. [Landi, Francesco] Catholic Univ Sacred Heart Rome, Dept Geriatr Neurosci & Orthoped, Milan, Italy. [Petermans, Jean] CHU Sart Tilman, Dept Geriatr, Bat B35, B-4000 Liege, Belgium. [Visser, Marjolein] Vrije Univ Amsterdam, Dept Hlth Sci, Amsterdam, Netherlands. [Visser, Marjolein] Vrije Univ Amsterdam, Med Ctr, Dept Nutr & Dietet, Internal Med, Amsterdam, Netherlands. [Kanis, John] Catholic Univ Australia, Inst Hlth & Aging, Melbourne, Vic, Australia. [Cooper, Cyrus] Univ Southampton, MRC Lifecourse Epidemiol Unit, Southampton, Hants, England. [Cooper, Cyrus] Univ Oxford, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. RP Beaudart, C (reprint author), Univ Liege, Dept Publ Hlth Epidemiol & Hlth Econ, Ave Hippocrate 13,CHU B23, B-4000 Liege, Belgium. EM c.beaudart@ulg.ac.be RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 FU Medical Research Council [MC_UP_A620_1014, MC_U147585819, MC_UU_12011/2, MC_UP_A620_1015, MC_UU_12011/1] NR 83 TC 1 Z9 1 U1 20 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2318 J9 BMC GERIATR JI BMC Geriatr. PD OCT 5 PY 2016 VL 16 AR 170 DI 10.1186/s12877-016-0349-4 PG 10 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DX9FS UT WOS:000384699600001 PM 27716195 ER PT J AU Margolis, LM Rivas, DA Berrone, M Ezzyat, Y Young, AJ McClung, JP Fielding, RA Pasiakos, SM AF Margolis, Lee M. Rivas, Donato A. Berrone, Maria Ezzyat, Yassine Young, Andrew J. McClung, James P. Fielding, Roger A. Pasiakos, Stefan M. TI Prolonged Calorie Restriction Downregulates Skeletal Muscle mTORC1 Signaling Independent of Dietary Protein Intake and Associated microRNA Expression SO FRONTIERS IN PHYSIOLOGY LA English DT Article DE muscle protein content; energy deficit; rpS6; miR-99; miR-100 ID RESISTANCE EXERCISE; SHORT-TERM; TRANSPORTER EXPRESSION; HYPERTROPHY; REPRESSION; INGESTION; INCREASE; LEUCINE; TARGET; BONE AB Short-term (5-10 days) calorie restriction (CR) downregulates muscle protein synthesis, with consumption of a high protein-based diet attenuating this decline. Benefit of increase protein intake is believed to be due to maintenance of amino acid-mediated anabolic signaling through the mechanistic target of rapamycin complex 1 (mTORC1), however, there is limited evidence to support this contention. The purpose of this investigation was to determine the effects of prolonged CR and high protein diets on skeletal muscle mTORC1 signaling and expression of associated microRNA (miR). Twelve-week old male Sprague Dawley rats consumed ad libitum (AL) or calorie restricted (CR; 40%) adequate (10%, AIN-93M) or high (32%) protein milk-based diets for 16 weeks. Body composition was determined using dual energy X-ray absorptiometry and muscle protein content was calculated from muscle homogenate protein concentrations expressed relative to fat-free mass to estimate protein content. Western blot and RT-qPCR were used to determine mTORC1 signaling and mRNA and miR expression in fasted mixed gastrocnemius. Independent of dietary protein intake, muscle protein content was 38% lower (P < 0.05) in CR compared to AL. Phosphorylation and total Akt, mTOR, rpS6, and p70S6K were lower (P < 0.05) in CR vs. AL, and total rpS6 was associated with muscle protein content (r = 0.64, r(2) = 0.36). Skeletal muscle miR expression was not altered by either energy or protein intake. This study provides evidence that chronic CR attenuates muscle protein content by downregulating mTORC1 signaling. This response is independent of skeletal muscle miR and dietary protein. C1 [Margolis, Lee M.; Rivas, Donato A.; Berrone, Maria; Ezzyat, Yassine; Fielding, Roger A.] Tufts Univ, Jean Mayer Human Nutr Res Ctr Aging, USDA, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Margolis, Lee M.; Young, Andrew J.; McClung, James P.; Pasiakos, Stefan M.] US Army, Mil Nutr Div, Environm Med Res Inst, Natick, MA 01760 USA. RP Pasiakos, SM (reprint author), US Army, Mil Nutr Div, Environm Med Res Inst, Natick, MA 01760 USA. EM stefan.m.pasiakos.civ@mail.mil RI Pasiakos, Stefan/E-6295-2014; OI Pasiakos, Stefan/0000-0002-5378-5820; Rivas, Donato/0000-0002-4500-6233; , Lee/0000-0002-0652-1304 FU US Army Medical Research and Material Command; Dairy Research Institute; USDA [58-1950-4-003]; T32 NIDDK training grant [5T32DK062032-23]; NIA [KAG047247A-A1] FX This material is based on the work supported by the US Army Medical Research and Material Command, the Dairy Research Institute, and the USDA under agreement No. 58-1950-4-003. LM was supported by the T32 NIDDK training grant # 5T32DK062032-23 and DR was supported by the NIA K01 award # KAG047247A-A1. NR 35 TC 1 Z9 1 U1 9 U2 9 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-042X J9 FRONT PHYSIOL JI Front. Physiol. PD OCT 5 PY 2016 VL 7 AR 445 DI 10.3389/fphys.2016.00445 PG 11 WC Physiology SC Physiology GA DX9CD UT WOS:000384690000001 PM 27761114 ER PT J AU Sundaram, S Yan, L AF Sundaram, Sneha Yan, Lin TI Dietary energy restriction reduces high-fat diet-enhanced metastasis of Lewis lung carcinoma in mice SO ONCOTARGET LA English DT Article DE energy restriction; high-fat diet; Lewis lung carcinoma; metastasis; mice ID BREAST-CANCER; TUMOR-GROWTH; RADICAL PROSTATECTOMY; WEIGHT-LOSS; OBESITY; OVERWEIGHT; INFLAMMATION; RECURRENCE; EXERCISE; IMPACT AB The objective of this study was to determine whether a reduction in energy intake ameliorated the high-fat diet-enhanced spontaneous metastasis of Lewis lung carcinoma in mice. Male C57BL/6 mice were fed the AIN93G diet, a high-fat diet or a high-fat diet with a 5% restriction of the intake. Energy restriction reduced body adiposity and body weight, but maintained growth similar to mice fed the AIN93G diet. The high-fat diet significantly increased the number and size (cross-sectional area and volume) of metastases formed in lungs. Restricted feeding reduced the number of metastases by 23%, metastatic cross-sectional area by 32% and volume by 45% compared to the high-fat diet. The high-fat diet elevated plasma concentrations of proinflammatory cytokines (monocyte chemotactic protein-1, plasminogen activator inhibitor-1, leptin), angiogenic factors (vascular endothelial growth factor, tissue inhibitor of metalloproteinase-1) and insulin. Restricted feeding significantly reduced the high-fat diet-induced elevations in plasma concentrations of proinflammatory cytokines, angiogenic factors and insulin. These results demonstrated that a reduction in diet intake by 5% reduced high-fat diet-enhanced metastasis, which may be associated with the mitigation of adiposity and down-regulation of cancer-promoting proinflammatory cytokines and angiogenic factors. C1 [Sundaram, Sneha; Yan, Lin] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Yan, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM lin.yan@ars.usda.gov FU U.S. Department of Agriculture, Agriculture Research Service [3062-51000-050-00D] FX Funding for this work was provided by the U.S. Department of Agriculture, Agriculture Research Service, Research Project 3062-51000-050-00D. NR 34 TC 0 Z9 0 U1 1 U2 1 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD OCT 4 PY 2016 VL 7 IS 40 BP 65669 EP 65675 DI 10.18632/oncotarget.11598 PG 7 WC Oncology; Cell Biology SC Oncology; Cell Biology GA EB3QQ UT WOS:000387281000087 PM 27582541 ER PT J AU Lupolova, N Dallman, TJ Matthews, L Bono, JL Gally, DL AF Lupolova, Nadejda Dallman, Timothy J. Matthews, Louise Bono, James L. Gally, David L. TI Support vector machine applied to predict the zoonotic potential of E-coli O157 cattle isolates SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE machine learning; zoonosis; Shiga toxin; E. coli; cattle ID HEMOLYTIC-UREMIC SYNDROME; PREVALENCE; SEROGROUPS; VIRULENCE; OUTBREAKS; SCOTLAND; O145; O111; O103; O26 AB Sequence analyses of pathogen genomes facilitate the tracking of disease outbreaks and allow relationships between strains to be reconstructed and virulence factors to be identified. However, these methods are generally used after an outbreak has happened. Here, we show that support vector machine analysis of bovine E. coli O157 isolate sequences can be applied to predict their zoonotic potential, identifying cattle strains more likely to be a serious threat to human health. Notably, only a minor subset (less than 10%) of bovine E. coli O157 isolates analyzed in our datasets were predicted to have the potential to cause human disease; this is despite the fact that the majority are within previously defined pathogenic lineages I or I/II and encode key virulence factors. The predictive capacity was retained when tested across datasets. The major differences between human and bovine E. coli O157 isolates were due to the relative abundances of hundreds of predicted prophage proteins. This finding has profound implications for public health management of disease because interventions in cattle, such a vaccination, can be targeted at herds carrying strains of high zoonotic potential. Machine-learning approaches should be applied broadly to further our understanding of pathogen biology. C1 [Lupolova, Nadejda; Gally, David L.] Univ Edinburgh, Roslin Inst, Div Immun & Infect, Roslin EH25 9RG, Midlothian, Scotland. [Lupolova, Nadejda; Gally, David L.] Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland. [Dallman, Timothy J.] Natl Infect Serv, Publ Hlth England, London NW9 5EQ, England. [Matthews, Louise] Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G12 8QQ, Lanark, Scotland. [Bono, James L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Gally, DL (reprint author), Univ Edinburgh, Roslin Inst, Div Immun & Infect, Roslin EH25 9RG, Midlothian, Scotland.; Gally, DL (reprint author), Univ Edinburgh, Royal Dick Sch Vet Studies, Roslin EH25 9RG, Midlothian, Scotland. EM dgally@ed.ac.uk OI Matthews, Louise/0000-0003-4420-8367 FU Food Standards Scotland and the Food Standards Agency Grant [FS101055]; University of Edinburgh studentship; core Biotechnology and Biological Sciences Research Council strategic programme [BB/J004227/1]; National Institute for Health Research Health Protection Research Unit in Gastrointestinal Infections at the University of Liverpool; Public Health England; University of East Anglia; University of Oxford; Institute of Food Research FX We would like to acknowledge the value of human and bovine E. coli O157 sequence data available from previous published studies, especially work from the Wellcome Trust IPRAVE consortium, Public Health England, and the Scottish E. coli reference laboratory. This work was supported by Food Standards Scotland and the Food Standards Agency Grant FS101055 (to D.L.G., T.J.D., and L.M.), which has allowed the continuation of significant EHEC O157 research in the UK. This research was also supported by a University of Edinburgh studentship (N.L.) and core Biotechnology and Biological Sciences Research Council strategic programme Grant BB/J004227/1 (to D.L.G.). T.J.D. was funded by the National Institute for Health Research Health Protection Research Unit in Gastrointestinal Infections at the University of Liverpool in partnership with Public Health England, University of East Anglia, University of Oxford, and the Institute of Food Research. NR 30 TC 2 Z9 2 U1 9 U2 9 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD OCT 4 PY 2016 VL 113 IS 40 BP 11312 EP 11317 DI 10.1073/pnas.1606567113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DX6XI UT WOS:000384528900075 PM 27647883 ER PT J AU Yahia, N Brown, CA Rapley, M Chung, M AF Yahia, Najat Brown, Carrie A. Rapley, Melyssa Chung, Mei TI Level of nutrition knowledge and its association with fat consumption among college students SO BMC PUBLIC HEALTH LA English DT Article DE Nutrition education; Fat consumption; Nutritional knowledge; University students ID AMERICAN-HEART-ASSOCIATION; UNIVERSITY-STUDENTS; GENDER-DIFFERENCES; PHYSICAL-ACTIVITY; FOOD CHOICE; BODY-WEIGHT; DISEASE; STROKE; FRUIT; PERCEPTION AB Background: Intake of saturated fat, trans fat, and cholesterol has been associated with increased risk of coronary heart disease. The aim of this study was to explore whether increased nutrition knowledge is associated with a reduction in the consumption of unhealthy fats in a sample of university students. Methods: A sample of 231 students, with a mean age of 20 years, was recruited from university campus during spring 2012. Students completed a validated questionnaire related to students' demographic, nutrition knowledge, and daily fat consumption. Weight, height, and waist circumference were measured. Data were analyzed using one-way ANOVA, chi-square, and student's t-test. Results: Results indicate that female students have greater nutrition knowledge than male students (the mean nutrition score for women was 5 points higher than that of men (P = 0.01)). Nutrition knowledge was negatively correlated with fat and cholesterol intake. Students who consumed more than 35 % calories from fat or >300 mg of cholesterol daily had lower mean nutrition scores than those students with lower fat or cholesterol intake (8 points lower and 7.9 points lower, respectively). Using linear regression for nutrition scores on estimated saturated fat intake and cholesterol intake (controlling for gender, height, weight, age, and dieting), nutrition scores were negatively associated with saturated fat intake (-0.15, P <0.0001) and cholesterol intake (-1.38, P <0.0001). Conclusion: Students with greater nutritional knowledge consumed less unhealthy fats and cholesterol. This finding magnifies the role of nutrition education as a potential tool in health campaigns to promote healthy eating patterns among college students. Results of this pilot study can inform the design of future nutrition education intervention studies to assess the efficacy of nutrition knowledge on pattern of fat consumption among college students. C1 [Yahia, Najat; Rapley, Melyssa] Cent Michigan Univ, Dept Human Environm Studies, Wightman 108, Mt Pleasant, MI 48859 USA. [Brown, Carrie A.] Tufts Univ, Jean Mayer USDA Human Nutr Ctr Aging, Boston, MA 02111 USA. [Chung, Mei] Tufts Univ, Dept Publ Hlth & Family Med, Sch Med, 136 Harrison Ave,Jaharis 264, Boston, MA 02111 USA. [Brown, Carrie A.] Boston Univ, Dept Biostat, 801 Massachusetts Ave, Boston, MA 02118 USA. RP Yahia, N (reprint author), Cent Michigan Univ, Dept Human Environm Studies, Wightman 108, Mt Pleasant, MI 48859 USA. EM yahia1n@cmich.edu FU Faculty Research and Creative Endeavors (FRCE) Premier Display grant at CMU FX This work was partially supported by the Faculty Research and Creative Endeavors (FRCE) Premier Display grant at CMU. NR 54 TC 0 Z9 0 U1 35 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD OCT 4 PY 2016 VL 16 AR 1047 DI 10.1186/s12889-016-3728-z PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DX9FW UT WOS:000384700000003 PM 27716127 ER PT J AU Sharma, T Dreyer, I Kochian, L Pineros, MA AF Sharma, Tripti Dreyer, Ingo Kochian, Leon Pineros, Miguel A. TI The ALMT Family of Organic Acid Transporters in Plants and Their Involvement in Detoxification and Nutrient Security SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE anion channel; ALMT; aluminum tolerance; nutrient transport; malate transport; citrate transport; review ID ACTIVATED MALATE TRANSPORTER; DEPENDENT ANION CHANNEL; WHEAT TRITICUM-AESTIVUM; ARABIDOPSIS HYPOCOTYL CELLS; ALUMINUM TOLERANCE; GUARD-CELLS; PLASMA-MEMBRANE; MALIC-ACID; STOMATAL MOVEMENT; CHLORIDE CHANNELS AB About a decade ago, members of a new protein family of anion channels were discovered on the basis of their ability to confer on plants the tolerance toward toxic aluminum ions in the soil. The efflux of Al3+-chelating malate anions through these channels is stimulated by external Al3+ ions. This feature of a few proteins determined the name of the entire protein family as Aluminum-activated Malate Transporters (ALMT). Meanwhile, after several years of research, it is known that the physiological roles of ALMTs go far beyond Al-detoxification. In this review article we summarize the current knowledge on this transporter family and assess their involvement in diverse physiological processes. C1 [Sharma, Tripti; Dreyer, Ingo] Univ Talca, Ctr Bioinformat & Simulac Mol, Fac Ingn, Talca, Chile. [Kochian, Leon; Pineros, Miguel A.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Dreyer, I (reprint author), Univ Talca, Ctr Bioinformat & Simulac Mol, Fac Ingn, Talca, Chile.; Pineros, MA (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM idreyer@utalca.cl; map25@cornell.edu RI Dreyer, Ingo/N-4747-2014; OI Pineros, Miguel/0000-0002-7166-1848 FU FONDECYT of the Comision Nacional Cientifica y Tecnologica of Chile [1150054]; National Science Foundation [NSF/IOS-1444435] FX This work was supported by the FONDECYT grant No 1150054 of the Comision Nacional Cientifica y Tecnologica of Chile (ID and TS) and National Science Foundation award NSF/IOS-1444435 (MP and LK). NR 89 TC 1 Z9 2 U1 26 U2 26 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD OCT 4 PY 2016 VL 7 AR 1488 DI 10.3389/fpls.2016.01488 PG 12 WC Plant Sciences SC Plant Sciences GA DX9CW UT WOS:000384692200001 PM 27757118 ER PT J AU Weller, TJ Castle, KT Liechti, F Hein, CD Schirmacher, MR Cryan, PM AF Weller, Theodore J. Castle, Kevin T. Liechti, Felix Hein, Cris D. Schirmacher, Michael R. Cryan, Paul M. TI First Direct Evidence of Long-distance Seasonal Movements and Hibernation in a Migratory Bat SO SCIENTIFIC REPORTS LA English DT Article ID TORPOR; ENERGETICS; TELEMETRY; LASIURUS; SONGBIRD; TAGS AB Understanding of migration in small bats has been constrained by limitations of techniques that were labor-intensive, provided coarse levels of resolution, or were limited to population-level inferences. Knowledge of movements and behaviors of individual bats have been unknowable because of limitations in size of tracking devices and methods to attach them for long periods. We used sutures to attach miniature global positioning system (GPS) tags and data loggers that recorded light levels, activity, and temperature to male hoary bats (Lasiurus cinereus). Results from recovered GPS tags illustrated profound differences among movement patterns by individuals, including one that completed a > 1000 km round-trip journey during October 2014. Data loggers allowed us to record sub-hourly patterns of activity and torpor use, in one case over a period of 224 days that spanned an entire winter. In this latter bat, we documented 5 torpor bouts that lasted >= 16 days and a flightless period that lasted 40 nights. These first uses of miniature tags on small bats allowed us to discover that male hoary bats can make multi-directional movements during the migratory season and sometimes hibernate for an entire winter. C1 [Weller, Theodore J.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. [Castle, Kevin T.] Wildlife Vet Consulting, 840 Sundance Dr, Livermore, CO 80536 USA. [Liechti, Felix] Swiss Ornithol Inst, Seerose 1, CH-6204 Sempach, Switzerland. [Hein, Cris D.; Schirmacher, Michael R.] Bat Conservat Int, POB 162603, Austin, TX 78716 USA. [Cryan, Paul M.] US Geol Survey, Ft Collins Sci Ctr, 2150 Ctr Ave,Bldg C, Ft Collins, CO 80526 USA. RP Weller, TJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM tweller@fs.fed.us OI Cryan, Paul/0000-0002-2915-8894 FU USDA Forest Service Pacific Southwest Research Station; U.S. Geological Survey, Fort Collins Science Center FX Funding for this project was provided by the USDA Forest Service Pacific Southwest Research Station and U.S. Geological Survey, Fort Collins Science Center. We are grateful to the California State Parks, North Coast Redwood District for granting us access and permission to conduct this study. We thank A. Brokaw, J. Clerc, T. Dewey, B. Fahey, M. Lau, C. Long, M. McKenzie, S. Mendia, M. Parker, K. Southall, V. Stover, J. Szewczak, and C. Zurek for invaluable assistance in the field. J. Baldwin prepared the figure on arousal times. Special thanks to R. Diehl for sacrificing his chance to deploy experimental data loggers on birds and instead contributing them to advancement of knowledge regarding the other class of extant flying vertebrates. Comments from B. Zielinski and J. Wolfe improved the manuscript. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 54 TC 0 Z9 0 U1 24 U2 24 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD OCT 4 PY 2016 VL 6 AR 34585 DI 10.1038/srep34585 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DX7DI UT WOS:000384546200001 PM 27698492 ER PT J AU Sainju, UM Lenssen, AW Allen, BL Stevens, WB Jabro, JD AF Sainju, Upendra M. Lenssen, Andrew W. Allen, Brett L. Stevens, William B. Jabro, Jalal D. TI Nitrogen balance in response to dryland crop rotations and cultural practices SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Cropping system; Management practice; Nitrogen input; Nitrogen output; Nitrogen budget; Soil total nitrogen ID SOIL-NITROGEN; LIVESTOCK SYSTEMS; SEMIARID MONTANA; N-FERTILIZATION; FOOD-PRODUCTION; TILLAGE; SEQUENCE; AGROECOSYSTEMS; LOSSES; CARBON AB Nitrogen balance provides a measure of agroecosystem performance and environmental sustainability by taking into accounts of N inputs and outputs and N retention in the soil. The objective of this study was to evaluate N balance based on N inputs and outputs and soil N sequestration after 7 yr in response to five dryland crop rotations (two 4-yr stacked and two 4-yr alternate-year rotations and one monocropping) and two cultural practices arranged in a split-plot design in the northern Great Plains, USA. Stacked rotations were durum (Triticum turgidum L.)-durum-canola (Brassica napus L)-pea (Pisum sativum L) (D-D-C-P) and durum-durum-flax (Linum usitadssimum L.)-pea (D-D-F-P). Alternate-year rotations were durum-canola-durum-pea (D-C-D-P) and durum-flax-durum-pea (D-F-D-P). Monocroppping was continuous durum (CD). Cultural practices were traditional (conventional till, recommended seed rate, broadcast N fertilization, and reduced stubble height) and ecological (no-till, increased seed rate, banded N fertilization, and increased stubble height). Total annual N input due to N fertilization, pea N fixation, atmospheric N deposition, crop seed N, and nonsymbiotic N fixation was lower in CD than other crop rotations, regardless of cultural practices. Total N output due to crop grain N removal and N losses due to denitrification, volatilization, plant senescence, N leaching, gaseous N (NO.) emissions, and surface runoff was lower in traditional CD and D-F-D-P than traditional D-C-D-P and ecological D-C-D-P, D-D-C-P, and D-F-D-P. Nitrogen sequestration rate at 0-125 cm from 2005 to 2011 ranged from 40 kg N ha(-1) yr(-1) for ecological D-D-F-P to 52 kg N ha(-1) yr(-1) for ecological CD. Nitrogen balance ranged from -39 to -36 kg N ha(-1) yr(-1) with CD compared to 9-25 kg N ha(-1) yr(-1) with other crop rotations in both cultural practices. Because of reduced reliance on external N inputs and increased grain N removal, N flow, and N surplus, crop rotations with legumes, nonlegumes, and oilseed crops in the rotation can be productive and environmentally sustainable compared with monocropping, regardless of cultural practices. Published by Elsevier B.V. C1 [Sainju, Upendra M.; Allen, Brett L.; Stevens, William B.; Jabro, Jalal D.] USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. [Lenssen, Andrew W.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov OI Sainju, Upendra/0000-0001-6943-733X NR 38 TC 0 Z9 0 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 25 EP 32 DI 10.1016/j.agee.2016.08.023 PG 8 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400003 ER PT J AU Ashworth, AJ Weiss, SA Keyser, PD Allen, FL Tyler, DD Taylor, A Beamer, KP West, CP Pote, DH AF Ashworth, A. J. Weiss, S. A. Keyser, P. D. Allen, F. L. Tyler, D. D. Taylor, A. Beamer, K. P. West, C. P. Pote, D. H. TI Switchgrass composition and yield response to alternative soil amendments under intensified heat and drought conditions SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Review DE Biochar; Legume intercropping; Alternative soil amendments; Climatic intensification; Feedstock composition; Plant adaptation ID CROPPING SYSTEMS; UNITED-STATES; COVER CROPS; BIOCHAR; MANAGEMENT; FEEDSTOCK; DYNAMICS AB Switchgrass (Panicum virgatum L.) and guinea grass (Panicum maximum Jacq.) have been proposed as sustainable alternatives to fossil fuels in subtropical and tropical environments, respectively; although still requiring non-renewable inputs, notably, fertilizer-nitrogen (N). Furthermore, climatic intensification forecasts suggest southeastern USA may emulate more tropical or subtropical growing conditions resulting in altered N dynamics and plant physiology. Objectives were to determine: (i) effects of biochar (1 and 2 Mg ha(-1)), two intercropped legumes [sunn hemp (Crotaiaria juncea L.) and pigeon pea (Cajanus cajan L) intercrops] versus inorganic N [67 kg ha-land 0 kg ha(-1)] on feedstock and soil characteristics and biomass yield; (ii) how feedstock composition is affected over three harvest dates; and, (iii) switchgrass adaptation to more extreme (tropical) growing conditions. For both species, yield and feedstock composition were influenced by harvest timing (P < 0.05), whereas soil amendments influenced composition to a lesser extent over the duration of this study (P > 0.05). In general, initial harvests had greater digestible 5- and 6-carbon sugars and N, P, and K tissue levels, whereas in subsequent harvests, higher acid- and neutral-detergent fiber levels were observed, suggesting lesser potential fermentability. Desired feedstock characteristics can therefore be manipulated by harvest timing. Yield results suggest pigeon pea and sunn hemp intercrops, and biochar (1 Mg ha(-1)) may result in equivalent yields as N fertilizer per harvest (P < 0.05). However, the 2 Mg ha(-1) biochar rate in the tropics adversely impacted yields, perhaps due to N immobilization by biochar. Switchgrass adaptation and competitiveness was moderate (5-30% weed cover) under an intensified climate. Growth can therefore be maintained under a stochastic climate due to its C-4 pathway and competitive growth on marginal soils. Published by Elsevier B.V. C1 [Ashworth, A. J.; Pote, D. H.] USDA ARS, Bumpers Small Farms Res Ctr, 6883 S Hwy 23, Booneville, AR 72927 USA. [Allen, F. L.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Keyser, P. D.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Weiss, S. A.; Beamer, K. P.] Univ Virgin Isl, Agr Expt Stn, St Croix, VI 00850 USA. [Tyler, D. D.] Univ Tennessee, Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA. [Taylor, A.] Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA. [West, C. P.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79410 USA. RP Ashworth, AJ (reprint author), USDA ARS, Bumpers Small Farms Res Ctr, 6883 S Hwy 23, Booneville, AR 72927 USA. EM Amanda.Ashworth@ars.usda.gov OI Ashworth, Amanda/0000-0002-3218-8939 FU Southeastern Sun Grant Center; U.S. Department of Transportation Research and Innovative Technology Administration [DTOS59-07-G-00050] FX Support for this research was provided by a grant from the Southeastern Sun Grant Center with funds provided by the U.S. Department of Transportation Research and Innovative Technology Administration (DTOS59-07-G-00050). In addition, authors thank the Agricultural Research and Education Centers in St. Croix, USVI and in Greenville, TN (Rob Ellis) and Knoxville, TN (Lee Ellis) for their help in collecting data and making this research possible. Lastly, gratitude is extended to the laboratory of Dr. Samy Sadaka at the University of Arkansas for the production of biochars used in field investigations. NR 36 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 415 EP 424 DI 10.1016/j.agee.2016.09.041 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400044 ER PT J AU Cobb, AB Wilson, GWT Goad, CL Bean, SR Kaufman, RC Herald, TJ Wilson, JD AF Cobb, Adam B. Wilson, Gail W. T. Goad, Carla L. Bean, Scott R. Kaufman, Rhett C. Herald, Thomas J. Wilson, Jeff D. TI The role of arbuscular mycorrhizal fungi in grain production and nutrition of sorghum genotypes: Enhancing sustainability through plant-microbial partnership SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Mycorrhizal fungi; Sorghum genotypes; Grain nutrition ID TALLGRASS PRAIRIE; WHEAT CULTIVARS; PHOSPHORUS; COLONIZATION; DOMESTICATION; COMMUNITIES; SYSTEMS; BICOLOR; STARCH AB The responsiveness of 3 landrace (open-pollinated) sorghum cultivars and 3 modern (commercial) hybrids to arbuscular mycorrhizal (AM) fungi and commercial fertilizers were assessed. Sorghum genotypes were selected to represent a range of sorghum breeding development. This comparison was conducted to link grain production and quality with crop nutrition strategies (AM symbiosis versus fertility amendments). The open-pollinated cultivars produced an average of 206% more vegetative biomass and 285% more grain per plant, compared to commercial hybrids when grown with AM fungi and no fertilization. Furthermore, the average protein production of open-pollinated cultivars was 320% greater, compared to hybrids grown under the same low-fertility conditions. Percent AM root colonization was 149% greater in open-pollinated cultivars compared to hybrids, and across all genotypes, AM colonization was significantly correlated with total grain mineral content. Sorghum cultivars were significantly more responsive to mycorrhizal symbiosis than commercial hybrids for nutrient uptake and subsequent grain production and quality, while hybrids were significantly more responsive to fertilization. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cobb, Adam B.; Wilson, Gail W. T.; Goad, Carla L.] Oklahoma State Univ, 008C AGH, Stillwater, OK 74078 USA. [Bean, Scott R.; Kaufman, Rhett C.; Herald, Thomas J.; Wilson, Jeff D.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. [Kaufman, Rhett C.] Bayer CropSci, 925 Cty Rd 378, Beaver Crossing, NE 68313 USA. RP Cobb, AB (reprint author), Oklahoma State Univ, 008C AGH, Stillwater, OK 74078 USA. EM abcobb@okstate.edu; gail.wilson@okstate.edu; carla.goad@okstate.edu; scott.bean@ars.usda.gov; rhett.kaufman@bayer.com; tom.herald@ars.usda.gov; jeff.d.wilson@ars.usda.gov FU Oklahoma State University, Department of Natural Resource Ecology and Management; Oklahoma Agriculture Experiment Station, Stillwater, Oklahoma, USA; USDA-ARS, Center for Grain and Animal Health Research, Manhattan, Kansas, USA FX This work was supported by Oklahoma State University, Department of Natural Resource Ecology and Management, and the Oklahoma Agriculture Experiment Station, Stillwater, Oklahoma, USA. Partial support received by USDA-ARS, Center for Grain and Animal Health Research, Manhattan, Kansas, USA. USDA collaborators conducted laboratory analysis, and interpretation. OSU collaborators were responsible for conducting the study, data collection, writing, and statistical analysis. All collaborators were involved in study design and editorial review of the article. NR 51 TC 0 Z9 0 U1 21 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD OCT 3 PY 2016 VL 233 BP 432 EP 440 DI 10.1016/j.agee.2016.09.024 PG 9 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA ED7YK UT WOS:000389088400046 ER PT J AU Haley, BJ Kim, SW Pettengill, J Luo, Y Karns, JS Van Kessel, JAS AF Haley, Bradd J. Kim, Seon Woo Pettengill, James Luo, Yan Karns, Jeffrey S. Van Kessel, Jo Ann S. TI Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America SO PLOS ONE LA English DT Article ID FIELD GEL-ELECTROPHORESIS; ANTIMICROBIAL RESISTANCE; POPULATION-STRUCTURE; EFFECTOR PROTEINS; VIBRIO-CHOLERAE; HEMOLYSIN-III; DAIRY FARM; SEROTYPES; TYPHIMURIUM; PREVALENCE AB Salmonella enterica subsp. enterica serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated S. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia. A phylogenetic analysis indicated that both STs are more closely related to other Salmonella serovars than they are to each other. Additionally, there was strong evidence of an evolutionary divergence between the poultry-associated and bovine-associated ST152 isolates that was due to polymorphisms in four core genome genes. The ST198 isolates recovered from dairy farms in the United States were phylogenetically distinct from those collected from human clinical cases with 66 core genome SNPs differentiating the two groups, but more isolates are needed to determine the significance of this distinction. Identification of S. Kentucky ST198 from dairy animals in the United States suggests that the presence of this pathogen should be monitored in food-producing animals. C1 [Haley, Bradd J.; Kim, Seon Woo; Karns, Jeffrey S.; Van Kessel, Jo Ann S.] ARS, Environm Microbial & Food Safety Lab, Beltsville Area Res Ctr, USDA, Beltsville, MD 20705 USA. [Pettengill, James; Luo, Yan] US FDA, Off Analyt & Outreach, Ctr Food Safety & Appl Nutr, College Pk, MD USA. RP Van Kessel, JAS (reprint author), ARS, Environm Microbial & Food Safety Lab, Beltsville Area Res Ctr, USDA, Beltsville, MD 20705 USA. EM joann.vankessel@ars.usda.gov FU internal USDA/ARS research funding FX This project was supported by internal USDA/ARS research funding.; We gratefully acknowledge Jakeitha Sonnier for laboratory assistance. This project was supported by internal USDA/ARS research funding. The mention of a trade name, proprietary product, or specific equipment does not constitute guarantee or warranty by the USDA and does not imply approval to the exclusion of other products that might be suitable. NR 62 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD OCT 3 PY 2016 VL 11 IS 10 AR e0161225 DI 10.1371/journal.pone.0161225 PG 36 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DZ0TR UT WOS:000385553100008 PM 27695032 ER PT J AU Li, W Katin-Grazzini, L Krishnan, S Thammina, C El-Tanbouly, R Yer, H Merewitz, E Guillard, K Inguagiato, J McAvoy, RJ Liu, ZR Li, Y AF Li, Wei Katin-Grazzini, Lorenzo Krishnan, Sanalkumar Thammina, Chandra El-Tanbouly, Rania Yer, Huseyin Merewitz, Emily Guillard, Karl Inguagiato, John McAvoy, Richard J. Liu, Zongrang Li, Yi TI A Novel Two-Step Method for Screening Shade Tolerant Mutant Plants via Dwarfism SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE mutation breeding; screening method; dwarfism; gibberellins; shade tolerance ID PERENNIAL RYEGRASS; TRINEXAPAC-ETHYL; TALL FESCUE; LIGHT; BERMUDAGRASS; RESPONSES; GROWTH; TURFGRASS; DROUGHT; STRESS AB When subjected to shade, plants undergo rapid shoot elongation, which often makes them more prone to disease and mechanical damage. Shade-tolerant plants can be difficult to breed; however, they offer a substantial benefit over other varieties in low light areas. Although perennial ryegrass (Lolium perenne L.) is a popular species of turf grasses because of their good appearance and fast establishment, the plant normally does not perform well under shade conditions. It has been reported that, in turfgrass, induced dwarfism can enhance shade tolerance. Here we describe a two-step procedure for isolating shade tolerant mutants of perennial ryegrass by first screening for dominant dwarf mutants, and then screening dwarf plants for shade tolerance. The two-step screening process to isolate shade tolerant mutants can be done efficiently with limited space at early seedling stages, which enables quick and efficient isolation of shade tolerant mutants, and thus facilitates development of shade tolerant new cultivars of turfgrasses. Using the method, we isolated 136 dwarf mutants from 300,000 mutagenized seeds, with 65 being shade tolerant (0.022%). When screened directly for shade tolerance, we recovered only four mutants from a population of 150,000 (0.003%) mutagenized seeds. One shade tolerant mutant, shadow-1, was characterized in detail. In addition to dwarfism, shadow-1 and its sexual progeny displayed high degrees of tolerance to both natural and artificial shade. We showed that endogenous gibberellin (GA) content in shadow-1 was higher than wild-type controls, and shadow-1 was also partially GA insensitive. Our novel, simple and effective two-step screening method should be applicable to breeding shade tolerant cultivars of turfgrasses, ground covers, and other economically important crop plants that can be used under canopies of existing vegetation to increase productivity per unit area of land. C1 [Li, Wei; Katin-Grazzini, Lorenzo; Thammina, Chandra; El-Tanbouly, Rania; Yer, Huseyin; Guillard, Karl; Inguagiato, John; McAvoy, Richard J.; Li, Yi] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. [Krishnan, Sanalkumar; Merewitz, Emily] Michigan State Univ, Dept Crop Sci, E Lansing, MI 48824 USA. [El-Tanbouly, Rania] Univ Alexandria, Fac Agr, Dept Floriculture Omamental Hort & Landscape Gard, Alexandria, Egypt. [Liu, Zongrang] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV USA. RP Li, Y (reprint author), Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. EM yi.li@uconn.edu FU Storrs Agricultural Experiment Station FX We thank the personnel at the University of Massachusetts for their kind assistance in gamma-ray irradiation of seed materials. We also thank Mr. Steve Olsen and his crew at the University of Connecticut, Plant Science Research and Education Facility for their help in growing and maintaining mutant plant populations. This project is financially supported by the Storrs Agricultural Experiment Station to YL. NR 40 TC 0 Z9 0 U1 16 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD OCT 3 PY 2016 VL 7 AR 1495 DI 10.3389/fpls.2016.01495 PG 12 WC Plant Sciences SC Plant Sciences GA DX6OV UT WOS:000384503900001 PM 27752260 ER PT J AU Nyarko, E Kniel, KE Millner, PD Luo, YG Handy, ET Reynnells, R East, C Sharma, M AF Nyarko, Esmond Kniel, Kalmia E. Millner, Patricia D. Luo, Yaguang Handy, Eric T. Reynnells, Russell East, Cheryl Sharma, Manan TI Survival and growth of Listeria monocytogenes on whole cantaloupes is dependent on site of contamination and storage temperature SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Listeria monocytogenes; Listeriosis; United States (US); Cantaloupes; Multistate; Outbreak ID FRESH-CUT CANTALOUPE; UNITED-STATES; MULTISTATE OUTBREAK; PREVENTION; SURFACES; QUALITY; PIECES; RIND AB Whole cantaloupes (Cucumis melo L.), marketed as 'Rocky Ford', were implicated in a large multi-state outbreak of listeriosis in the United States in 2011; however, survival and growth of Listeria monocytogenes on whole cantaloupes remains relatively unexplored. The research presented here evaluated three different storage temperatures, two sites of contamination of cantaloupes, and two cantaloupe varieties to determine their effect on the survival of L. monocytogenes. 'Athena' and 'Rocky Ford' cantaloupe cultivars were grown in soil and harvested, and individual melons subsequently received a multi-strain inoculum of L. monocytogenes (6 log CFU/melon), which were then stored at 4 degrees C, 10 degrees C, and 25 degrees C. Changes in L monocytogenes populations on the rinds and stem scars of cantaloupes stored at each temperature were determined at selected times for up to 15 days. An analysis of variance revealed that inoculation site and storage temperature significantly affected survival of L monocytogenes on cantaloupes during storage (p < 0.05), but cultivar did not influence L monocytogenes (p > 0.05). Populations of L monocytogenes on stem scars of cantaloupes stored at 25 degrees C increased by 1-2 log CFU/melon on day 1, and were significantly greater than those on cantaloupes stored at 4 degrees C or 10 degrees C (p < 0.05), which remained constant or increased by approximately 03 log CFU/melon, respectively, over the same time period. A decrease of 2-5 log CFU/melon of L. monocytogenes occurred on the rinds of cantaloupes during storage by day 7, and were not significantly different at the three different storage temperatures (p > 0.05). In trials performed in rind juice extracts, populations of L. monocytogenes decreased by 3 log CFU/mL when stored at 25 degrees C by day 3, but grew by 3-4 log CFU/mL when stored at 4 degrees C over 7 days. Overall, site of contamination and storage temperature influenced the survival of L. monocytogenes on cantaloupes more than cantaloupe cultivar type. Published by Elsevier B.V. C1 [Nyarko, Esmond; Kniel, Kalmia E.] Univ Delaware, Dept Anim & Food Sci, 044 Townsend Hall 531 S Coll Ave, Newark, DE 19716 USA. [Millner, Patricia D.; Luo, Yaguang; Handy, Eric T.; Reynnells, Russell; East, Cheryl; Sharma, Manan] United States Dept Agr, Agr Res Serv, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Sharma, M (reprint author), United States Dept Agr, Agr Res Serv, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM esmond.nyarko@ars.usda.gov; kniel@udel.edu; pat.millner@ars.usda.gov; yaguang.luo@ars.usda.gov; eric.handy@ars.usda.gov; cheryl.east@ars.usda.gov; manan.sharma@ars.usda.gov FU FDA [224-11-2046]; USDA ARS [224-11-2046, 58-1245-4-111, 8042-32420-005-00]; University of Delaware [58-1245-4-111] FX The authors would like to thank Patrick Spanninger and Dr. Gordon Johnson at the University of Delaware, Newark and Georgetown, Delaware; Kate White, Dave Clark, and Richard Stonebraker at the Environmental Microbial and Food Safety Laboratory, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, Maryland; and Yi Chen, Dumitru Macarisin, Michael Mahovic, Annemarie Buchholz, and Mickey Parish at the Center for Food Safety and Applied Nutrition (CFSAN), U.S. Food and Drug Administration (U.S. FDA) for their assistance with this study. This study was funded by Inter-Agency Agreement 224-11-2046 between FDA and USDA ARS, Specific Cooperative Agreement 58-1245-4-111 between USDA ARS and University of Delaware, and USDA ARS Project number 8042-32420-005-00 "Microbial Ecology and Safety of Fresh Produce". NR 22 TC 2 Z9 2 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD OCT 3 PY 2016 VL 234 BP 65 EP 70 DI 10.1016/j.ijfoodmicro.2016.06.030 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DV0IE UT WOS:000382600400009 PM 27376678 ER PT J AU Sabo, R Yermakov, A Law, CT Elhajjar, R AF Sabo, Ronald Yermakov, Aleksey Law, Chiu Tai Elhajjar, Rani TI Nanocellulose-Enabled Electronics, Energy Harvesting Devices, Smart Materials and Sensors: A Review SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Cellulose nanofibrils; cellulose nanocrystals; flexible electronics; photovoltaics; batteries; magnetostrictive composites ID OPTICALLY TRANSPARENT COMPOSITES; LI-ION BATTERIES; BACTERIAL CELLULOSE; THIN-FILMS; TERFENOL-D; MICROFIBRILLATED CELLULOSE; CARBON NANOTUBE; MAGNETOSTRICTIVE ACTUATORS; BIOMEDICAL APPLICATIONS; FLEXIBLE ELECTRONICS AB Cellulose nanomaterials have a number of interesting and unique properties that make them well-suited for use in electronics applications such as energy harvesting devices, actuators and sensors. Cellulose nanofibrils and nanocrystals have good mechanical properties, high transparency, and low coefficient of thermal expansion, among other properties that facilitate both active and inactive roles in electronics and related devices. For example, these nanomaterials have been demonstrated to operate as substrates for flexible electronics and displays, to improve the efficiency of photovoltaics, to work as a component of magnetostrictive composites and to act as a suitable lithium ion battery separator membrane. A discussion and overview of additional potential applications and of previously published research using cellulose nanomaterials for these advanced applications is provided in this article. The concept of using cellulose nanofibrils in stimuli-responsive materials is illustrated with highlights of preliminary results from magnetostrictive nanocellulose membranes actuated using magnetic fields. C1 [Sabo, Ronald] US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Yermakov, Aleksey] Univ Wisconsin, Dept Mech Engn, Milwaukee, WI 53211 USA. [Law, Chiu Tai] Univ Wisconsin, Dept Elect Engn & Comp Sci, Milwaukee, WI 53211 USA. [Elhajjar, Rani] Univ Wisconsin, Dept Civil & Environm Engn, Milwaukee, WI 53211 USA. RP Sabo, R (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM rsabo@fs.fed.us NR 134 TC 0 Z9 0 U1 2 U2 2 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 297 EP 312 DI 10.7569/JRM.2016.634114 PG 16 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EL6RX UT WOS:000394750900001 ER PT J AU Lara, PN Plets, M Tangen, C Gertz, E Vogelzang, NJ Quinn, DI Thompson, I van Loan, M AF Lara, P. N. Plets, M. Tangen, C. Gertz, E. Vogelzang, N. J. Quinn, D. I. Thompson, I. van Loan, M. TI Bone biomarkers and overall survival (OS) in men with castrate resistant prostate cancer (CRPC) and skeletal metastases: Updated results from SWOG 0421, a phase III trial of docetaxel +/- atrasentan SO ANNALS OF ONCOLOGY LA English DT Meeting Abstract CT 41st Congress of the European-Society-for-Medical-Oncology (ESMO) CY OCT 07-11, 2016 CL Copenhagen, DENMARK SP European Soc Med Oncol C1 [Lara, P. N.] Univ Calif Davis, Ctr Canc, Internal Med Hematol Oncol, Sacramento, CA 95817 USA. [Plets, M.; Tangen, C.] Fred Hutchinson Canc Res Ctr, Stat, 1124 Columbia St, Seattle, WA 98104 USA. [Gertz, E.; van Loan, M.] Univ Calif Davis, USDA, Davis, CA 95616 USA. [Vogelzang, N. J.] Comprehens Canc Crts NV, US Oncol Res, Med Oncol, Las Vegas, NV USA. [Quinn, D. I.] Univ Southern Calif, Norris Comprehens Canc Ctr, Med, Los Angeles, CA USA. [Thompson, I.] Univ Texas Hlth Sci Ctr San Antonio, Urol, San Antonio, TX 78229 USA. FU National Cancer Institute (USA) FX National Cancer Institute (USA) NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0923-7534 EI 1569-8041 J9 ANN ONCOL JI Ann. Oncol. PD OCT 1 PY 2016 VL 27 SU 6 MA 754P DI 10.1093/annonc/mdw372.38 PG 1 WC Oncology SC Oncology GA EK4QS UT WOS:000393912500751 ER PT J AU Preece, JE Moersfelder, J AF Preece, John E. Moersfelder, Jeff TI Pomegranate: The Grainy Apple SO JOURNAL OF THE AMERICAN POMOLOGICAL SOCIETY LA English DT Article DE Punica granatum; fruit quality; germplasm C1 [Preece, John E.; Moersfelder, Jeff] Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, One Shields Ave, Davis, CA 95616 USA. RP Preece, JE (reprint author), Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, One Shields Ave, Davis, CA 95616 USA. EM John.Preece@ars.usda.gov NR 23 TC 0 Z9 0 U1 1 U2 1 PU AMER POMOLOGICAL SOC PI UNIVERSITY PK PA 102 TYSON BUILDING, UNIVERSITY PK, PA 16802 USA SN 1527-3741 J9 J AM POMOL SOC JI J. Amer. Pomolog. Soc. PD OCT PY 2016 VL 70 IS 4 BP 187 EP 193 PG 7 WC Agronomy; Horticulture SC Agriculture GA EJ9ZE UT WOS:000393585300003 ER PT J AU Kaur, N Hasegawa, DK Ling, KS Wintermantel, WM AF Kaur, Navneet Hasegawa, Daniel K. Ling, Kai-Shu Wintermantel, William M. TI Application of Genomics for Understanding Plant Virus-Insect Vector Interactions and Insect Vector Control SO PHYTOPATHOLOGY LA English DT Article ID WHITEFLY BEMISIA-TABACI; INFECTIOUS-YELLOWS-VIRUS; DEEP SEQUENCING ANALYSIS; TOMATO-CHLOROSIS-VIRUS; LEAF-CURL-VIRUS; RNA INTERFERENCE; FRANKLINIELLA-OCCIDENTALIS; SPECIES COMPLEX; AEDES-AEGYPTI; COAT PROTEIN AB The relationships between plant viruses and their vectors have evolved over the millennia, and yet, studies on viruses began <150 years ago and investigations into the virus and vector interactions even more recently. The advent of next generation sequencing, including rapid genome and transcriptome analysis, methods for evaluation of small RNAs, and the related disciplines of proteomics and metabolomics offer a significant shift in the ability to elucidate molecular mechanisms involved in virus infection and transmission by insect vectors. Genomic technologies offer an unprecedented opportunity to examine the response of insect vectors to the presence of ingested viruses through gene expression changes and altered biochemical pathways. This review focuses on the interactions between viruses and their whitefly or thrips vectors and on potential applications of genomics-driven control of the insect vectors. Recent studies have evaluated gene expression in vectors during feeding on plants infected with begomoviruses, criniviruses, and tospoviruses, which exhibit very different types of virus-vector interactions. These studies demonstrate the advantages of genomics and the potential complementary studies that rapidly advance our understanding of the biology of virus transmission by insect vectors and offer additional opportunities to design novel genetic strategies to manage insect vectors and the viruses they transmit. C1 [Kaur, Navneet; Wintermantel, William M.] USDA ARS, Crop Improvement & Protect Res, Salinas, CA 93905 USA. [Hasegawa, Daniel K.; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Hasegawa, Daniel K.] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14853 USA. RP Wintermantel, WM (reprint author), USDA ARS, Crop Improvement & Protect Res, Salinas, CA 93905 USA. EM bill.wintermantel@ars.usda.gov FU USDA-ARS Area-Wide project grant; U.S. Agency for International Development (USAID) Feed-the-Future program [58-0210-3-012] FX This work was supported in part by a USDA-ARS Area-Wide project grant to K.-S. Ling and W. M. Wintermantel, and a grant from the U.S. Agency for International Development (USAID) Feed-the-Future program (58-0210-3-012) coordinated by the USDA-ARS Office of International Research Programs. NR 108 TC 1 Z9 1 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1213 EP 1222 DI 10.1094/PHYTO-02-16-0111-FI PG 10 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100016 ER PT J AU Clemons, C Pilla, S AF Clemons, Craig Pilla, Srikanth TI Introduction to the Special Issue on Nanocellulose Composites SO JOURNAL OF RENEWABLE MATERIALS LA English DT Editorial Material C1 [Clemons, Craig] US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. [Pilla, Srikanth] Clemson Univ, Clemson, SC 29631 USA. RP Clemons, C (reprint author), US Forest Serv, USDA, Forest Prod Lab, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 295 EP 296 DI 10.7569/JRM.2016.634133 PG 2 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400001 ER PT J AU Stark, NM AF Stark, Nicole M. TI Opportunities for Cellulose Nanomaterials in Packaging Films: A Review and Future Trends SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Cellulose nanocrystals (CNCs); cellulose nanofibrils (CNFs); cellulose nanomaterials; packaging; films; composites; mechanical properties; barrier properties; water vapor transmission rate; oxygen transmission rate; modification ID WATER-VAPOR BARRIER; MICROFIBRILLATED CELLULOSE; NANOCOMPOSITE FILMS; POLY(LACTIC ACID); NANOCRYSTALLINE CELLULOSE; POSIDONIA-OCEANICA; PLA NANOCOMPOSITES; OPTICAL-PROPERTIES; POLYVINYL-ALCOHOL; COMPOSITE FILMS AB Performance requirements for packaging films may include barrier properties, transparency, flexibility, and tensile strength. Conventional packaging materials, such as plastic films and laminates, are typically made from petroleum-based polymers. Currently, there is a drive to develop sustainable packaging materials. These alternative materials must be able to be manufactured economically and on a commercial scale, exhibit barrier properties and transparency, and provide adequate mechanical performance. As a biobased, renewable material, cellulose nanomaterials (CNs) are ideally suited to be used in sustainable packaging applications. CNs include cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) and each can provide benefit to packaging films. Manufactured CNF films can be used as packaging films or laminates and have been shown to have good strength properties and good barrier properties, particularly at low humidity. Both CNCs and CNFs can be added to other polymers to improve strength and barrier properties. The flexibility of CNs to be used in a variety of ways in packaging applications has resulted in considerable attention and research activity. This article summarizes the current applicability for CNs in packaging films and discusses the future trends and opportunities for these materials. C1 [Stark, Nicole M.] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Stark, NM (reprint author), US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM nstark@fs.fed.us NR 96 TC 0 Z9 0 U1 8 U2 8 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 313 EP 326 DI 10.7569/JRM.2016.634115 PG 14 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400003 ER PT J AU Clemons, C AF Clemons, Craig TI Nanocellulose in Spun Continuous Fibers: A Review and Future Outlook SO JOURNAL OF RENEWABLE MATERIALS LA English DT Review DE Nanocellulose; cellulose nanofibrils; cellulose nanocrystals; fiber; spinning ID REGENERATED CELLULOSE FIBERS; POLYVINYL-ALCOHOL; ELECTROSPUN NANOFIBERS; REINFORCED COMPOSITES; POLY(ETHYLENE OXIDE); POLY(VINYL ALCOHOL); NANOCRYSTALS; NANOCOMPOSITES; WHISKERS; NANOWHISKERS AB Continuous fibers are commonly manufactured for a wide variety of uses such as filters, textiles, and composites. For example, most fibrous reinforcements (e.g., carbon fiber, glass fiber) for advanced composites are continuous fibers or yarns, fabrics, and preforms made from them. This allows broad flexibility in design and manufacturing approaches by controlling fiber orientation and architecture. However, there has been growing interest in preparing continuous fibers from biobased materials such as plants. Of particular recent interest are nanocelluloses, which are projected to be less expensive than many other nanomaterials and have the potential to be produced in large volumes. They also have an impressive strength-to-weight ratio and have so far shown few environmental, health, and safety concerns in their unmodified state. However, efficient and effective use of nanocellulose in continuous fibers is challenging and a variety of approaches have been explored in which nanocellulose dispersions are either spun directly or in combination with polymers. Methods such as wet spinning, dry spinning, melt spinning, and electrospinning have been investigated. To better understand the body of knowledge of this new and growing area, various approaches are reviewed and a perspective on what the future holds is provided. C1 [Clemons, Craig] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Clemons, C (reprint author), US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM cclemons@fs.fed.us NR 71 TC 1 Z9 1 U1 12 U2 12 PU SCRIVENER PUBLISHING LLC PI BEVERLY PA 100 CUMMINGS CENTER, SUITE 541J, BEVERLY, MA 01915-6106 USA SN 2164-6325 EI 2164-6341 J9 J RENEW MATER JI J. Renew. Mater. PD OCT PY 2016 VL 4 IS 5 BP 327 EP 339 DI 10.7569/JRM.2016.634112 PG 13 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Composites; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA EJ3SH UT WOS:000393131400004 ER PT J AU Eguiguren-Velepucha, PA Chamba, JAM Mendoza, NAA Ojeda-Luna, TL Samaniego-Rojas, NS Furniss, MJ Howe, C Mendoza, ZHA AF Alexander Eguiguren-Velepucha, Paul Maita Chamba, Juan Armando Aguirre Mendoza, Nikolay Arturo Lizbeth Ojeda-Luna, Tatiana Soledad Samaniego-Rojas, Natalia Furniss, Michael J. Howe, Carol Aguirre Mendoza, Zhofre Huberto TI Tropical ecosystems vulnerability to climate change in southern Ecuador SO TROPICAL CONSERVATION SCIENCE LA English DT Article DE biodiversity; drivers of change; exposure; sensitivity; adaptive capacity ID CHANGE IMPACTS; BIODIVERSITY; CONSERVATION; DISTRIBUTIONS; COMMUNITIES; PREDICTIONS; CHALLENGES; MODELS; FUTURE; GROWTH AB Tropical ecosystems are among the most vulnerable to climate change. Understanding climate impacts on these ecosystems is a primary challenge for policy makers, ecologists, and conservationists today. We analyzed the vulnerability of ecosystems in a very heterogeneous tropical region in southern Ecuador, selected because of its exceptional biodiversity and its ecosystem services provided to people of southern Ecuador and northern Peru. The vulnerability assessment focused on three components: exposure, sensitivity, and adaptive capacity. For the first two components, we identified stressors or drivers of change that negatively influence ecosystems. For the third component, we identified existing and potential buffers that reduce impacts. This process was developed in workshops and by expert elicitation. Representative Concentration Pathway (RCP) scenarios were used, considering RCP 2.6 and RCP 8.5 for a time horizon to 2050. Under the RCP 2.6 scenario, the components of overall vulnerability in the southern region of Ecuador showed very low to moderate vulnerability for most areas, particularly in semi-deciduous forest ecosystems, Amazon semi-deciduous forest, Amazon rainforest, and mangrove forests. These areas had high vulnerability under the RCP 8.5 scenario. A variety of conservation strategies (e.g., protected areas) were shown to increase the adaptive capacity of ecosystems and reduce their vulnerability. We therefore recommend improving these conservation initiatives in ecosystems like dry forests, where the greatest vulnerability is evident. C1 [Alexander Eguiguren-Velepucha, Paul; Maita Chamba, Juan Armando; Aguirre Mendoza, Nikolay Arturo; Lizbeth Ojeda-Luna, Tatiana; Soledad Samaniego-Rojas, Natalia] Univ Nacl Loja, Biodivers & Ecosyst Serv Res Program, EC-110103 Loja, Ecuador. [Furniss, Michael J.] Humboldt State Univ, Dept Environm Engn, Arcata, CA 95521 USA. [Furniss, Michael J.; Howe, Carol] US Forest Serv, USDA, Washington, DC 20250 USA. [Aguirre Mendoza, Zhofre Huberto] Univ Nacl Loja, Herbarium Loja Reinaldo Espinosa, Loja, Ecuador. RP Eguiguren-Velepucha, PA (reprint author), Univ Nacl Loja, Biodivers & Ecosyst Serv Res Program, EC-110103 Loja, Ecuador. EM pauleguiguren@gmail.com FU United States Agency for International Development FX We thank Universidad Nacional de Loja, the United States Forest Service for the scientific advisor; and the United States Agency for International Development for the financial support. NR 80 TC 0 Z9 0 U1 5 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1940-0829 J9 TROP CONSERV SCI JI Trop. Conserv. Sci. PD OCT-DEC PY 2016 VL 9 IS 4 DI 10.1177/1940082916668007 PG 17 WC Biodiversity Conservation SC Biodiversity & Conservation GA EJ4SS UT WOS:000393207800004 ER PT J AU Burley, JD Bytnerowicz, A Buhler, M Zielinska, B Schweizer, D Cisneros, R Schilling, S Varela, JC McDaniel, M Horn, M Dulen, D AF Burley, Joel D. Bytnerowicz, Andrzej Buhler, Monica Zielinska, Barbara Schweizer, Donald Cisneros, Ricardo Schilling, Susan Varela, Jennifer Chapman McDaniel, Mark Horn, Michelle Dulen, Deanna TI Air Quality at Devils Postpile National Monument, Sierra Nevada Mountains, California, USA SO AEROSOL AND AIR QUALITY RESEARCH LA English DT Article DE Ozone; Passive samplers; PM2.5; VOC; Nitrogen oxides ID SURFACE OZONE; PARTICULATE MATTER; DRY DEPOSITION; WILDLAND FIRE; VARIABILITY; ENVIRONMENT; POLLUTION; FORESTS; DESERT; HEALTH AB Ambient concentrations of O-3, PM2.5, NH3, NO, NO2, HNO3, SO2 and VOCs were measured at Devils Postpile National Monument (DEPO) during the summer seasons of 2013 and 2014. The measurements were impacted by the Aspen and Rim Fires in 2013, and the French and King Fires in 2014. While O-3 concentrations were not discernibly perturbed by the fire events, the 70 ppb threshold (8-hour average) corresponding to both the current California Ambient Air Quality Standard (CAAQS) and the new National Ambient Air Quality Standard (NAAQS) was exceeded on five days during 2013, and on 16 days during 2014. The older NAAQS of 75 ppb (8-hour average) was exceeded once in 2013, and six times in 2014. Exceedances of the CAAQS or NAAQS occurred when background sources of O-3 were augmented by regional-scale transport, at higher altitudes, of polluted air masses that had passed through the San Joaquin Valley before arriving at the DEPO site. The 2013 Aspen Fire elevated PM2.5 to a maximum hourly concentration of 214 mu g m(-3) and a maximum 24 h mean of 92.7 mu g m(-3), and resulted in 13 exceedances of the 35 mu g m(-3) (24 h average) NAAQS for PM2.5. The 2013 Rim Fire increased PM2.5 to a maximum hourly concentration of 132 mu g m(-3) and a maximum 24 h mean of 69.6 mu g m(-3), and resulted in two exceedances of the 24 h NAAQS. Concentrations of NH3 increased during all fires, as did those of NO2 during the Aspen and Rim Fires. Concentrations of benzene increased substantially during the French Fire. C1 [Burley, Joel D.; Varela, Jennifer Chapman] St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. [Bytnerowicz, Andrzej; Schilling, Susan] USDA Forest Serv, PSW Res Stn, Riverside, CA 92507 USA. [Buhler, Monica; Dulen, Deanna] Devils Postpile Natl Monument, Mammoth Lakes, CA 93546 USA. [Zielinska, Barbara; McDaniel, Mark] Desert Res Inst, Reno, NV 89512 USA. [Schweizer, Donald; Cisneros, Ricardo] Univ Calif, Merced, CA 95340 USA. [Horn, Michelle] Tech Univ Munich, D-85354 Freising Weihenstephan, Germany. RP Burley, JD (reprint author), St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. EM jburley@stmarys-ca.edu FU USDA Forest Service Pacific Southwest Research Station; National Park Service FX This study was funded by the USDA Forest Service Pacific Southwest Research Station and the National Park Service. We thank Diane Alexander, Reginald King, Mark Williams, Diana Lopez and Pegdwende Kologo for preparing and analyzing passive samplers, and Catherine Fong and Andrew Osborne for passive sampler deployment and changes. The authors gratefully acknowledge the NOAA Air Resources Laboratory (ARL) for the provision of the HYSPLIT transport and dispersion model and/or READY website (http://www.ready.noaa.gov) used in this publication. NR 52 TC 0 Z9 0 U1 0 U2 0 PU TAIWAN ASSOC AEROSOL RES-TAAR PI TAICHUNG COUNTY PA CHAOYANG UNIV TECH, DEPT ENV ENG & MGMT, PROD CTR AAQR, NO 168, JIFONG E RD, WUFONG TOWNSHIP, TAICHUNG COUNTY, 41349, TAIWAN SN 1680-8584 EI 2071-1409 J9 AEROSOL AIR QUAL RES JI Aerosol Air Qual. Res. PD OCT PY 2016 VL 16 IS 10 BP 2315 EP 2332 DI 10.4209/aaqr.2016.02.0069 PG 18 WC Environmental Sciences SC Environmental Sciences & Ecology GA EI2GK UT WOS:000392304700001 ER PT J AU Kesoju, SR Boydston, RA Greene, SL AF Kesoju, Sandya R. Boydston, Rick A. Greene, Stephanie L. TI Effect of Synthetic Auxin Herbicides on Seed Development and Viability in Genetically Engineered Glyphosate-Resistant Alfalfa SO WEED TECHNOLOGY LA English DT Article DE Adventitious presence; feral; gene flow; genetically engineered; glyphosate resistant; synthetic auxin herbicides ID MEDICAGO-SATIVA L.; NO-TILLAGE CORN; GENE FLOW; MANAGEMENT; FECUNDITY; ECOLOGY; PROGENY; TRAITS; GROWTH; 2,4-D AB Feral populations of cultivated crops have the potential to function as bridges and reservoirs that contribute to the unwanted movement of novel genetically engineered (GE) traits. Recognizing that feral alfalfa has the potential to lower genetic purity in alfalfa seed production fields when it is growing in the vicinity of foraging pollinators in alfalfa seed fields, industry has established production standards to control feral plants. However, with the commercialization of GE glyphosate-resistant (GR) alfalfa and the need to support the coexistence of both GE and conventional production, effective methods to control transgenic feral alfalfa need to be developed. Therefore, a study was conducted in 2012, 2013, and 2014 to determine the effect of several synthetic auxin herbicides on seed development in GR alfalfa. GR alfalfa, var. Genuity (R44BD16), was treated with dicamba, 2,4-D, triclopyr, and aminopyralid when alfalfa plants contained green seed pods. Two weeks after herbicide application, plants were harvested, air dried, and seed yield, seed germination, and seedling emergence from the soil were determined. In 2013, dicamba, triclopyr, and 2,4-D decreased alfalfa seed yield per plant compared wih nontreated plants, whereas in 2014, all four herbicides decreased alfalfa seed yield per plant 24 to 49% (by weight) compared with nontreated plants. The same trend was evident in 2012, but seed yield was variable and was not significantly different among treatments. Seed germination averaged 43, 50, and 72% in 2012, 2013, and 2014, respectively, and was not affected by the four herbicides applied at early pod-fill stage. However, seeds harvested from plants treated with dicamba, 2,4-D, and triclopyr often produced deformed and abnormal seedlings, and when planted in soil, frequently failed to emerge. The combined effects of dicamba, 2,4-D, and triclopyr in reducing seed yield, seedling emergence, and seedling growth could contribute to managing feral alfalfa populations. C1 [Kesoju, Sandya R.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Boydston, Rick A.] ARS, Legume Genet & Physiol Res Unit, USDA, Prosser, WA 99350 USA. [Greene, Stephanie L.] ARS, Plant & Anim Genet Resources Preservat Unit, USDA, Ft Collins, CO 80521 USA. RP Kesoju, SR (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. EM sandya.kesoju@wsu.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture [2011-33522-30733] FX The authors are grateful to Biotechnology Risk Assessment Grant Program # 2011-33522-30733 from the U.S. Department of Agriculture-National Institute of Food and Agriculture for funding of this research. We thank Treva Anderson and Encarnacion Rivera for their technical assistance and Bernardo Chaves-Cordoba for statistical analysis of the data. NR 42 TC 0 Z9 0 U1 1 U2 1 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD OCT-DEC PY 2016 VL 30 IS 4 BP 860 EP 868 DI 10.1614/WT-D-16-00045.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA EI4EZ UT WOS:000392447200004 ER PT J AU Solaiman, DKY Ashby, RD Crocker, NV AF Solaiman, Daniel K. Y. Ashby, Richard D. Crocker, Nicole V. TI Genetic construction of recombinant Pseudomonas chlororaphis for improved glycerol utilization SO BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY LA English DT Article DE Glycerol metabolism; Biodegradable polymer; Biosurfactant ID AERUGINOSA; METABOLISM AB This study is to use genetic engineering to improve the glycerol metabolic capability of Pseudomonas chlororaphis which is capable of producing commercially valuable biodegradable poly(hydroxyalkanoate) (PHA) biopolymers and biosurfactant rhamnolipids (RLs). In the study, the glycerol uptake facilitator or aquaglyceroporin gene (glpF) and the glycerol kinase (g1pK) gene were PCR-cloned from E. coli, inserted into a shuttle vector pBS29P2-gfp, and expressed in P. chlororaphis by a Pseudomonas promoter P2. The P. chlororaphis recombinants were then tested for cell growth and glycerol metabolism in chemically defined medium containing 0.5% and 1.0% (v/v) glycerol. The simultaneous expression of glpF and glpK resulted in a shorter lag time for cell growth and a more immediate glycerol consumption by P. chlororaphis. In conclusion, the recombinant P. chlororaphis that grows more efficiently in glycerol is expected to improve the technoeconomics of PHA and RL production using the surplus bioglycerol byproduct stream from biodiesel production. (C) 2016 Published by Elsevier Ltd. C1 [Solaiman, Daniel K. Y.; Ashby, Richard D.; Crocker, Nicole V.] ARS, Eastem Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Solaiman, DKY (reprint author), ARS, Eastem Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM dan.solaiman@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1878-8181 J9 BIOCATAL AGRIC BIOTE JI Biocatal. Agric. Biotechnol. PD OCT PY 2016 VL 8 BP 45 EP 49 DI 10.1016/j.bcab.2016.08.001 PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA EG2NP UT WOS:000390880700008 ER PT J AU Qu, WC Robert, CAM Erb, M Hibbard, BE Paven, M Gleede, T Riehl, B Kersting, L Cankaya, AS Kunert, AT Xu, Y Schueller, MJ Shea, C Alexoff, D Lee, SJ Fowler, JS Ferrieri, RA AF Qu, Wenchao Robert, Christelle A. M. Erb, Matthias Hibbard, Bruce E. Paven, Maxim Gleede, Tassilo Riehl, Barbara Kersting, Lena Cankaya, Aylin S. Kunert, Anna T. Xu, Youwen Schueller, Michael J. Shea, Colleen Alexoff, David Lee, So Jeong Fowler, Joanna S. Ferrieri, Richard A. TI Dynamic Precision Phenotyping Reveals Mechanism of Crop Tolerance to Root Herbivory SO PLANT PHYSIOLOGY LA English DT Article ID WESTERN CORN-ROOTWORM; AUXIN BIOSYNTHESIS; RAPID CHANGES; ARABIDOPSIS-THALIANA; CARBON TRANSPORT; MICROPET R4; ACID; PLANTS; TRYPTOPHAN; DAMAGE AB The western corn rootworm (WCR; Diabrotica virgifera virgifera LeConte) is a major pest of maize (Zea mays) that is well adapted to most crop management strategies. Breeding for tolerance is a promising alternative to combat WCR but is currently constrained by a lack of physiological understanding and phenotyping tools. We developed dynamic precision phenotyping approaches using C-11 with positron emission tomography, root autoradiography, and radiometabolite flux analysis to understand maize tolerance to WCR. Our results reveal that WCR attack induces specific patterns of lateral root growth that are associated with a shift in auxin biosynthesis from indole-3-pyruvic acid to indole-3-acetonitrile. WCR attack also increases transport of newly synthesized amino acids to the roots, including the accumulation of Gln. Finally, the regrowth zones of WCR-attacked roots show an increase in Gln turnover, which strongly correlates with the induction of indole-3-acetonitrile-dependent auxin biosynthesis. In summary, our findings identify local changes in the auxin biosynthesis flux network as a promising marker for induced WCR tolerance. C1 [Qu, Wenchao; Xu, Youwen; Schueller, Michael J.; Shea, Colleen; Alexoff, David; Lee, So Jeong; Fowler, Joanna S.; Ferrieri, Richard A.] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. [Robert, Christelle A. M.; Erb, Matthias] Univ Bern, Inst Plant Sci, Biot Interact, CH-3013 Bern, Switzerland. [Hibbard, Bruce E.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Paven, Maxim; Gleede, Tassilo; Riehl, Barbara; Kersting, Lena; Cankaya, Aylin S.; Kunert, Anna T.] Johannes Gutenberg Univ Mainz, Inst Kernchem, D-55128 Mainz, Germany. [Qu, Wenchao] Cornell Univ, Weill Cornell Med, New York, NY 10065 USA. [Paven, Maxim] Max Planck Inst Polymer Res, Ackermannweg 10, D-55128 Mainz, Germany. [Kunert, Anna T.] Max Planck Inst Chem, Hahn Meitner Weg 1, D-55128 Mainz, Germany. [Ferrieri, Richard A.] Univ Missouri, Dept Chem, MURR, 1513 Res Pk Dr, Columbia, MO 65211 USA. [Lee, So Jeong] Univ Michigan, Dept Radiol, Med Ctr Dr, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA. RP Ferrieri, RA (reprint author), Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA.; Ferrieri, RA (reprint author), Univ Missouri, Dept Chem, MURR, 1513 Res Pk Dr, Columbia, MO 65211 USA. EM ferrierir@missouri.edu FU United States Department of Energy, Office of Biological and Environmental Research [DE-AC02-98CH10886]; German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn; Marie Curie Intra-European Fellowship program; Swiss National Foundation Fellowship program; United States Government FX This article has been authored by Brookhaven Science Associates, LLC, under contract number DE-AC02-98CH10886 with the United States Department of Energy, Office of Biological and Environmental Research. Additional support was provided by the German Academic Exchange Service (Deutscher Akademischer Austauschdienst, DAAD) Bonn, the Marie Curie Intra-European Fellowship program, and the Swiss National Foundation Fellowship program. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a nonexclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. NR 54 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD OCT PY 2016 VL 172 IS 2 BP 776 EP 788 DI 10.1104/pp.16.00735 PG 13 WC Plant Sciences SC Plant Sciences GA EG6II UT WOS:000391147700013 PM 27406166 ER PT J AU Bo, KL Wang, H Pan, YP Behera, TK Pandey, S Wen, CL Wang, YH Simon, PW Li, YH Chen, JF Weng, YQ AF Bo, Kailiang Wang, Hui Pan, Yupeng Behera, Tusar K. Pandey, Sudhakar Wen, Changlong Wang, Yuhui Simon, Philipp W. Li, Yuhong Chen, Jinfeng Weng, Yiqun TI SHORT HYPOCOTYL1 Encodes a SMARCA3-Like Chromatin Remodeling Factor Regulating Elongation SO PLANT PHYSIOLOGY LA English DT Article ID TRANSCRIPTION FACTOR HY5; LIGHT SIGNAL-TRANSDUCTION; B PHOTORECEPTOR UVR8; SOLAR ULTRAVIOLET-RADIATION; SYNTHASE GENE-EXPRESSION; CUCUMBER CUCUMIS-SATIVUS; QUANTITATIVE TRAIT LOCI; ARABIDOPSIS-THALIANA; BLUE-LIGHT; HYPOCOTYL GROWTH AB In Arabidopsis (Arabidopsis thaliana), the UVR8-mediated signaling pathway is employed to attain UVB protection and acclimation to deal with low-dosage UVB (LDUVB)-induced stresses. Here, we identified SHORT HYPOCOTYL1 (SH1) in cucumber (Cucumis sativus), which regulates LDUVB-dependent hypocotyl elongation by modulating the UVR8 signaling pathway. We showed that hypocotyl elongation in cucumbers carrying the recessive sh1 allele was LDUVB insensitive and that Sh1 encoded a human SMARCA3-like chromatin remodeling factor. The allele frequency and distribution pattern at this locus among natural populations supported the wild cucumber origin of sh1 for local adaptation, which was under selection during domestication. The cultivated cucumber carries predominantly the Sh1 allele; the sh1 allele is nearly fixed in the semiwild Xishuangbanna cucumber, and the wild cucumber population is largely at Hardy-Weinberg equilibrium for the two alleles. The SH1 protein sequence was highly conserved among eukaryotic organisms, but its regulation of hypocotyl elongation in cucumber seems to be a novel function. While Sh1 expression was inhibited by LDUVB, its transcript abundance was highly correlated with hypocotyl elongation rate and the expression level of cell-elongation-related genes. Expression profiling of key regulators in the UVR8 signaling pathway revealed significant differential expression of CsHY5 between two near isogenic lines of Sh1. Sh1 and CsHY5 acted antagonistically at transcriptional level. A working model was proposed in which Sh1 regulates LDUVB-dependent hypocotyl elongation in cucumber through changing the chromatin states and thus the accessibility of CsHY5 in the UVR8 signaling pathway to promoters of LDUVB-responsive genes for hypocotyl elongation. C1 [Bo, Kailiang; Pan, Yupeng; Wang, Yuhui; Simon, Philipp W.; Weng, Yiqun] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Bo, Kailiang; Chen, Jinfeng] Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Hui; Pan, Yupeng; Li, Yuhong] Northwest A&F Univ, Hort Coll, Yangling 712100, Peoples R China. [Behera, Tusar K.] Indian Agr Res Inst, Div Vegetable Sci, New Delhi 10012, India. [Pandey, Sudhakar] Indian Inst Vegetable Res, Indian Council Agr Res, Div Crop Improvement, Varanasi 221305, Uttar Pradesh, India. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Simon, Philipp W.; Weng, Yiqun] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Chen, JF (reprint author), Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China.; Weng, YQ (reprint author), ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. EM jfchen@njau.edu.cn; yiqun.weng@wisc.edu FU United States Department of Agriculture Specialty Crop Research Initiative grant (SCRI) [2011-51181-30661]; National Natural Science Foundation of China [31171955, 31471891, 31430075] FX This work was supported by a United States Department of Agriculture Specialty Crop Research Initiative grant (SCRI, project no. 2011-51181-30661) to Y.We. The work in Y.L.'s lab was supported by the National Natural Science Foundation of China (31171955 and 31471891). J.C.'s work was supported by the National Natural Science Foundation of China (31430075). NR 108 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD OCT PY 2016 VL 172 IS 2 BP 1273 EP 1292 DI 10.1104/pp.16.00501 PG 20 WC Plant Sciences SC Plant Sciences GA EG6II UT WOS:000391147700049 PM 27559036 ER PT J AU Neto, TDN Gardner, D Hallwass, F Leite, AJM de Almeida, CG Silva, LN Roque, AD de Bitencourt, FG Barbosa, EG Tasca, T Macedo, AJ de Almeida, MV Giordani, RB AF Negreiros Neto, Themistocles da Silva Gardner, Dale Hallwass, Fernando Matias Leite, Ana Jessica de Almeida, Camila Guimaraes Silva, Laura Nunes Roque, Alan de Araujo de Bitencourt, Fernanda Gobbi Barbosa, Euzebio Guimaraes Tasca, Tiana Macedo, Alexandre Jose de Almeida, Mauro Vieira Giordani, Raquel Brandt TI Activity of pyrrolizidine alkaloids against biofilm formation and Trichomonas vaginalis SO BIOMEDICINE & PHARMACOTHERAPY LA English DT Article DE Usaramine; Monocrotaline; Pyrrolizidine alkaloids; Biological activity ID BRAZILIAN SEMIARID REGION; BACTERIAL BIOFILMS; DOUBLE-BLIND; INFECTIONS; VIRULENCE; OINTMENT; SYMPHYTI; EFFICACY; CAATINGA AB Crotalaria genus belongs to the subfamily Papilionoideae comprising about 600 species spread throughout tropical, neotropical and subtropical regions. In this study, seeds of Crolatalaria pallida were used to the isolation of usaramine, a pyrrolizidine alkaloid. Thus, Pseudomonas aeruginosa and Staphylococcus epidermidis were utilized as strains to test some activities of this alkaloid, such as antibiofilm and antibacterial. Meanwhile, monocrotaline obtained from Crotalaria retusa seeds, was used as the starting material for synthesis of necine base derivatives with anti-Trichomonas vaginalis potential. Alkaloids were characterized by 1D and 2D NMR techniques and GC-MS analysis. Usaramine demonstrated a highlighted antibiofilm activity against S. epidermidis by reducing more than 50% of biofilm formation without killing the bacteria, thus it could be assumed as a prototype for the development of new antibiofilm molecules for pharmaceutical and industrial purposes. Monocrotaline activity against T. vaginalis was evaluated and results indicated inhibition of 80% on parasite growth at 1 mg/mL, in addition, neither cytotoxicity against vaginal epithelial cells nor hemolytic activity were observed. On the other hand, retronecine showed no anti-T. vaginalis activity while azido-retronecine was more active than monocrotaline killing 85% of the parasites at 1 mg/mL. In conclusion, pyrrolizidine alkaloids are suggested as promising prototypes for new drugs especially for topical use. (C) 2016 Elsevier Masson SAS. All rights reserved. C1 [Negreiros Neto, Themistocles da Silva; Matias Leite, Ana Jessica; Barbosa, Euzebio Guimaraes; Giordani, Raquel Brandt] Univ Fed Rio Grande do Norte, Dept Farm, Gustavo Cordeiro de Faria SN, BR-59010180 Natal, RN, Brazil. [Gardner, Dale] ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Hallwass, Fernando] Univ Fed Pernambuco, Dept Quim Fundamental, Ave Prof Moraes Rego 1235, BR-50670901 Recife, PE, Brazil. [de Almeida, Camila Guimaraes; de Almeida, Mauro Vieira] Univ Fed Juiz de Fora, Inst Ciencias Exatas, Dept Quim, Campus Martelos, BR-36036330 Juiz De Fora, MG, Brazil. [Silva, Laura Nunes; Macedo, Alexandre Jose] Univ Fed Rio Grande do Sul, Ctr Biotecnol, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. [Silva, Laura Nunes; Macedo, Alexandre Jose] Univ Fed Rio Grande do Sul, Fac Farm, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. [Roque, Alan de Araujo] Univ Fed Rio Grande do Norte, Herbario UFRN, Campus Univ Lagoa Nova, BR-59078970 Natal, RN, Brazil. [de Bitencourt, Fernanda Gobbi; Tasca, Tiana] Univ Fed Rio Grande do Sul, Fac Farm, Lab Pesquisa Parasitol, Ave Ipiranga 2752, BR-90610000 Porto Alegre, RS, Brazil. RP Giordani, RB (reprint author), Univ Fed Rio Grande do Norte, Dept Farm, Gustavo Cordeiro de Faria SN, BR-59010180 Natal, RN, Brazil. EM theminegreiros@yahoo.com.br; dale.gardner@ars.usda.gov; hallwass@ufpe.br; ana@analeite.org; camilagdealmeida@gmail.com; lauransilva@gmail.com; alan.ufrn@gmail.com; fe_gobbi@hotmail.com; euzebiobg@gmail.com; tiana.tasca@ufrgs.br; alexandre.macedo@ufrgs.br; mauro.almeida@ufjf.edu.br; raquebg@hotmail.com FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil; CNPq (Brazil) [471209/2011-2]; FAPEMIG; NANOBIOTEC-Brazil program from CAPES; FAPERGS; CNPq [307447/2014-6] FX T.S.N.N. acknowledgethe Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil, for research fellowship. This work was supported by a CNPq (Brazil) grant [471209/2011-2] awarded to R.B.G., FAPEMIG and by the NANOBIOTEC-Brazil program from CAPES and FAPERGS awarded to A.J.M. T.T. thanks CNPq for researcher fellowship (grant 307447/2014-6). NR 35 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0753-3322 EI 1950-6007 J9 BIOMED PHARMACOTHER JI Biomed. Pharmacother. PD OCT PY 2016 VL 83 BP 323 EP 329 DI 10.1016/j.biopha.2016.06.033 PG 7 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA EF6IC UT WOS:000390433400040 ER PT J AU Njoroge, AW Affognon, H Mutungi, C Rohde, B Richter, U Hensel, O Mankin, RW AF Njoroge, A. W. Affognon, H. Mutungi, C. Rohde, B. Richter, U. Hensel, O. Mankin, R. W. TI Frequency and time pattern differences in acoustic signals produced by Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae) and Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) in stored maize SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Detection; Sensor; Pest management; Postharvest ID RHYNCHOPHORUS-FERRUGINEUS; GRAIN; INFESTATION; ORYZAE; PESTS; WHEAT; DRYOPHTHORIDAE; DETECTABILITY; OVIPOSITION; POPULATIONS AB Frequency spectra and timing patterns of brief, 1-10 ms broadband sound impulses produced by movement and feeding activities of Prostephanus truncatus and Sitophilus zeamais last instars and adults in maize were investigated to find spectral and temporal pattern information useful for distinguishing among these species and stages. The impulse spectra were categorized into five different types of frequency patterns (profiles), designated Broadband, HighF, MidF1, MidF2 and LowF to indicate differences in their peak energies and broadness of frequency range. Groups (trains) of three or more closely spaced impulses, termed bursts, were observed to occur frequently in all recordings, as has been reported for sounds produced by other insects. Mean rates of bursts, mean counts of impulses per burst, and mean rates of impulses in bursts were calculated and compared among the two species and stages. The counts of broadband and MidF2 impulses per burst and the rates of broadband and MidF2 impulses in bursts were significantly different for adult than for 4th instar S. zeamais and either stage of P. truncatus. These findings can be useful in developing an acoustic sensor system for automated detection of hidden insects including P. truncatus and S. zeamais in bulk storage warehouses. The findings are discussed in relation to different movement and feeding behavior patterns that have been identified in these important pests. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Njoroge, A. W.; Mutungi, C.] Int Ctr Insect Physiol & Ecol, POB 30772, Nairobi 00100, Kenya. [Njoroge, A. W.; Richter, U.; Hensel, O.] Univ Kassel, Fac Agr Engn, Nordbahnhofstr 1a, D-37213 Witzenhausen, Germany. [Affognon, H.] Int Crops Res Inst Semi Arid Trop, BP 320, Bamako, Mali. [Rohde, B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. [Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Njoroge, AW (reprint author), POB 30772, Nairobi 00100, Kenya. EM anjoroge@icipe.org NR 43 TC 1 Z9 1 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD OCT PY 2016 VL 69 BP 31 EP 40 DI 10.1016/j.jspr.2016.06.005 PG 10 WC Entomology SC Entomology GA EF7MY UT WOS:000390514900006 ER PT J AU Yee, WL Goughnour, RB AF Yee, Wee L. Goughnour, Robert B. TI Peach is an occasional host for Rhagoletis pomonella (Walsh, 1867) (Diptera: Tephritidae) larvae in western Washington state, USA SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE apple maggot; Prunus persica; Malus domestica ID CHERRY FRUIT-FLY; APPLE MAGGOT; PLANT USE AB Peach, Prunus persica (L.) Batsch (Rosaceae), has been reported to be a host of the apple maggot fly, Rhagoletis pomonella (Walsh, 1867) (Diptera: Tephritidae), an important quarantine pest of apple (Malus domestica Borkhausen) (Rosaceae) in the western U.S.A. However, all reports of peach as a host appear unreliable. Here, we tested the hypothesis that apple maggot does not utilize peaches in western Washington state. In 2013, unbaited sticky yellow rectangle traps were hung in a peach orchard similar to 50 m from neglected apple trees in Vancouver, Washington. During 2013-2015, peaches in the orchard were collected, peaches were hung in infested apple trees, and peaches were exposed to flies in the laboratory. In 2013, 24 flies were caught on 200 traps in the peach orchard, but no larvae emerged from 4,243 'Alberta' or 'Red-haven' peaches collected under similar to 200 trees in 2013 and 2014 or from peaches hung in apple trees in 2013. In 2015, however, 24 apple maggot larvae emerged from 804 `Starfire' or `PF 23' peaches, specifically from collections under two of 13 trees. In addition, in one of four laboratory tests, 11 larvae emerged from 'Redhaven' peaches exposed to apple maggot flies. Contrary to our prediction, results definitively show that peach can be an occasional developmental host for apple maggot larvae in the field and that apple maggot flies can be successfully reared using peach as a host in the laboratory. C1 [Yee, Wee L.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Goughnour, Robert B.] Washington State Univ, CAHNRS Extens, Vancouver, WA 98665 USA. RP Yee, WL (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov; Robert.Goughnour@clark.wa.gov FU USDA-Foreign Agricultural Service FX We thank Doug Stienbarger at Washington State University Vancouver for providing research facilities, Peter Chapman for taking the photographs of the puparia, Gene Miliczky for obtaining walnut husk fly puparia, Michael Klaus (Washington State Department of Agriculture) and Juan Rull (PROIMI Biotecnologia-CONICET, LIEMEN-Division Control Biologico de Plagas, Tucuman, Argentina) for reviewing earlier drafts of the manuscript, Brad Sinclair (Canadian National Collection of Insects and Ottawa Plant Laboratory - Entomology, Canadian Food Inspection Agency, Ottawa, Ontario, Canada) and an anonymous reviewer for valuable comments on the manuscript, and the USDA-Foreign Agricultural Service for partially funding this work. NR 27 TC 0 Z9 0 U1 1 U2 1 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD OCT PY 2016 VL 92 IS 4 BP 189 EP 199 PG 11 WC Entomology SC Entomology GA EG3XD UT WOS:000390977100002 ER PT J AU Pratt, PD Herdocia, K Valentin, V Makinson, J Purcell, MF Mattison, E Rayamajhi, MB Moran, P Raghu, S AF Pratt, P. D. Herdocia, K. Valentin, V. Makinson, J. Purcell, M. F. Mattison, E. Rayamajhi, M. B. Moran, P. Raghu, S. TI Development rate, consumption, and host fidelity of Neostauropus alternus (Walker, 1855) (Lepidoptera: Notodontidae) SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Host use; weed biological control; assimilation; Rhodomyrtus tomentosa ID RHODOMYRTUS-TOMENTOSA MYRTACEAE; WHEAT CURL MITE; BIOLOGICAL-CONTROL; COMPLEX; WEED; SPECIATION; INSECTS; ACARI AB The Asian shrub Rhodomyrtus tomentosa (Aiton) Hasskarl (Myrtaceae) is an invasive weed in Florida and Hawaii, U.S.A. Surveys for natural enemies of this exotic shrub in Hong Kong, China resulted in the development of a laboratory colony and host range testing of Neostauropus alternus (Walker, 1855) as a potential biological control agent of R. tomentosa. Twelve critical test plant species were presented to larvae of N alternus. Complete development was limited to R. tomentosa, the ornamentally important Myrtus communis Linneaus (Myrtaceae), and the Florida natives Myrcianthes fragrans (Small) (K.A. Wilson) (Myrtaceae) and Morella cerifera (Linneaus) Small (Myricaceae). Total development time was more than ten days faster with R tomentosa versus M. communis and M. cerifera, with the latter species requiring an extra larval instar to reach the pupal stage. Consumption rates were similar among R. tomentosa and M. cerifera, but due to the longer development time, larvae consumed two-fold more leaf material on M. cerifera. Despite an apparent larval survival and development preference for R. tomentosa, it is clear that the physiological host range of N alternus includes M. cerifera and M. communis. The generalized feeding patterns exhibited in this research indicate that additional resources dedicated to the development of N alternus as a biological control agent of R. tomentosa in Florida are unwarranted. C1 [Pratt, P. D.; Moran, P.] USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Herdocia, K.; Valentin, V.] St Thomas Univ, Dept Biol, 16401 NW 37th Ave, Miami Gardens, FL 33054 USA. [Makinson, J.; Purcell, M. F.; Raghu, S.] USDA ARS, Australian Biol Control Lab, CSIRO, GPO Box 2583, Brisbane, Qld 4001, Australia. [Mattison, E.; Rayamajhi, M. B.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. RP Pratt, PD (reprint author), USDA ARS, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM Paul.Pratt@ars.usda.gov; kherdocia@stu.edu; vvalentin@stu.edu; Makinson@csiro.au; Matthew.Purcell@csiro.au; Elizabeth.Mattison@ars.usda.gov; Min.Raymajhi@ars.usda.gov; Patrick.Moran@ars.usda.gov; Raghu.Sathyamurthy@csiro.au FU St. Thomas University; USDA-HSI FX We thank two anonymous reviewers for comments on earlier versions of the manuscript. The authors thank the support of St. Thomas University and the USDA-HSI funded grant consortium Florida-Caribbean Consortium for Agriculture Education, its staff, and the USDA agency directors and supervisors. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity employer and provider. NR 34 TC 0 Z9 0 U1 2 U2 2 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD OCT PY 2016 VL 92 IS 4 BP 200 EP 209 PG 10 WC Entomology SC Entomology GA EG3XD UT WOS:000390977100003 ER PT J AU Carvalho, LB Duke, SO Messa, JR Costa, FR Bianco, S AF Carvalho, L. B. Duke, S. O. Messa, J. R. Costa, F. R. Bianco, S. TI PLANT GROWTH RESPONSES OF APPLE AND PEAR TREES TO DOSES OF GLYPHOSATE SO PLANTA DANINHA LA English DT Article DE N-phosphonomethyl-glycine; Malus domestica; Pyrus communis; spray drift ID RYEGRASS LOLIUM-MULTIFLORUM; EPICUTICULAR WAX; RESISTANCE; TOLERANCE; MECHANISMS; ROOTSTOCKS; CULTIVARS; BIOTYPES; ARABICA; CROPS AB Glyphosate is commonly used for intra-row weed management in perennial plantations, where unintended crop exposure to this herbicide can cause growth reduction. The objective of this research was to analyze the initial plant growth behavior of young apple and pear plants exposed to glyphosate. Glyphosate was sprayed on 2-year-old 'Gala' apple and 'Abbe Fetel' pear plants at doses from 18 to 720 g per hectare of acid equivalent (a.e.). The plant height of neither species was not significantly reduced (less than 1%) by any glyphosate dose at 240 days after spraying, whereas the stem diameter and the dry mass of stem and leaves were reduced by 720 g a.e. ha(-1). The glyphosate dose required to reduce the aboveground dry mass by 50% was 162 and 148 g a.e. ha-1 for apple and pear, respectively. Aboveground dry mass was reduced 2% and 6% for apple and pear plants, respectively, at 720 g a.e. ha(-1). Hormesis was not observed in either species at doses down to 18 g a.e. ha(-1). Both species showed low susceptibility to glyphosate; however apple was less susceptible than pear. C1 [Carvalho, L. B.; Messa, J. R.; Costa, F. R.] Univ Estado Santa Catarina, Lages, SC, Brazil. [Duke, S. O.] Univ Mississippi, USDA, Oxford, MS USA. [Bianco, S.] Univ Estadual Paulista, Jaboticabal, SP, Brazil. RP Carvalho, LB (reprint author), Univ Estado Santa Catarina, Lages, SC, Brazil. EM lbcarvalho@dracena.unesp.br FU CNPq (PIBIC/CNPq Scholarship Program) from the Brazilian Government; CAPES (Master Scholarship Program) from the Brazilian Government FX The authors thank CNPq (PIBIC/CNPq Scholarship Program) and CAPES (Master Scholarship Program) from the Brazilian Government for supporting this research. NR 42 TC 0 Z9 0 U1 9 U2 9 PU UNIV FEDERAL VICOSA PI VICOSA PA CAIXA POSTAL 270, VICOSA, MG CEP 36571-00, BRAZIL SN 0100-8358 EI 1806-9681 J9 PLANTA DANINHA JI Planta Daninha PD OCT-DEC PY 2016 VL 34 IS 4 BP 815 EP 822 DI 10.1590/S0100-83582016340400022 PG 8 WC Plant Sciences SC Plant Sciences GA EF6JE UT WOS:000390436500022 ER PT J AU Miyazawa, Y Dudley, BD Hughes, RF Vandemark, J Cordell, S Nullet, MA Ostertag, R Giambelluca, TW AF Miyazawa, Yoshiyuki Dudley, Bruce D. Hughes, R. Flint Vandemark, Joshua Cordell, Susan Nullet, Michael A. Ostertag, Rebecca Giambelluca, Thomas W. TI Non-native tree in a dry coastal area in Hawai'i has high transpiration but restricts water use despite phreatophytic trait SO ECOHYDROLOGY LA English DT Article DE coastal groundwater; carbon assimilation; invasive species; sap flow; stomatal control ID SAP FLOW MEASUREMENTS; STOMATAL CONDUCTANCE; PHOTOSYNTHETIC CAPACITY; EUCALYPTUS-GLOBULUS; SEASONAL-VARIATION; CO2 ASSIMILATION; PROSOPIS-PALLIDA; HYDRAULIC LIMITS; VEGETATION TYPES; CARBON-DIOXIDE AB In arid systems, phreatophytes are often among the most effective invaders because of their capacity to access and exploit groundwater resources otherwise unavailable to native vegetation. On Hawai'i Island, a non-native phreatophyte, Prosopis pallida (kiawe), has invaded extensive dry lowland areas following its introduction in the 19th century. To better understand the influence of this invader on the host ecosystem, and vice versa, we determined transpiration characteristics of P. pallida by monitoring sap flux of mature individuals over an 18-month period on the arid leeward coast of Hawai'i Island. Daily sap flux increased with increasing atmospheric vapour pressure deficit (D) and exhibited no clear response related to rainfall events or prolonged drought. Annual transpiration (i.e. 308 mm) was 80% higher than rainfall. Stomatal conductance decreased with increasing vapour pressure deficits more slowly than the theoretical stomatal conductance-D relationship that assumes stomatal regulation of leaf water potential above a critical level. While daily peak stomatal conductance was relatively high, it nonetheless appeared limited by high intrinsic water use efficiency (net photosynthetic rate per stomatal conductance), suggesting a constraint to unlimited groundwater absorption. In this young volcanic environment where rainfall is low and quite episodic, the success of this species likely stems from its anisohydric strategy even at the risk of cavitation, and it is altering the hydrological cycling of the areas where it is dominant. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Miyazawa, Yoshiyuki; Nullet, Michael A.; Giambelluca, Thomas W.] Univ Hawaii Manoa, Dept Geog, Honolulu, HI 96822 USA. [Miyazawa, Yoshiyuki] Kyushu Univ, Res Inst East Asia Environm, 744 Motooka, Fukuoka 8190395, Japan. [Dudley, Bruce D.; Hughes, R. Flint; Vandemark, Joshua; Cordell, Susan] US Forest Serv, Inst Pacific Isl Forestry, USDA, 60 Nowelo St, Hilo, HI 96720 USA. [Dudley, Bruce D.; Vandemark, Joshua; Ostertag, Rebecca] Univ Hawaii, Dept Biol, 200 W Kawili St, Hilo, HI 96720 USA. [Dudley, Bruce D.] Natl Inst Water & Atmospher Res, 10 Kyle St, Christchurch, New Zealand. RP Miyazawa, Y (reprint author), Kyushu Univ, Res Inst East Asia Environm, 744 Motooka, Fukuoka 8190395, Japan. EM sclerophyll@gmail.com OI Giambelluca, Thomas/0000-0002-6798-3780 FU NSF - Hawai'i EPSCoR [EPS-0903833]; USDA Forest Service Pacific Southwest Research Station; JSPS [20-7278]; Excellent Young Researchers Overseas Visit Program for research at the University of Hawai'i at Manoa FX We thank Dr Creighton M. Litton (University of Hawai'i at Manoa) for allowing us to use instruments for field measurements, Hui Aloha Kiholo community group for their support of this research and the Hawai'i Department of Land and Natural Resources staff for providing access to Kiholo State Park. This study was supported by funding from NSF - Hawai'i EPSCoR grant no. EPS-0903833 and received in-kind support from the USDA Forest Service Pacific Southwest Research Station. This study was partly supported by the JSPS to Y. Miyazawa (20-7278) and Excellent Young Researchers Overseas Visit Program for research at the University of Hawai'i at Manoa. NR 64 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD OCT PY 2016 VL 9 IS 7 BP 1166 EP 1176 DI 10.1002/eco.1715 PG 11 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA EF0NF UT WOS:000390021700003 ER PT J AU Lopez-Martinez, G Meagher, RL Jeffers, LA Bailey, WD Hahn, DA AF Lopez-Martinez, Giancarlo Meagher, Robert L. Jeffers, Laura A. Bailey, Woodward D. Hahn, Daniel A. TI Low oxygen atmosphere enhances post-irradiation survival of Trichoplusia ni (Lepidoptera: Noctuidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE cross-tolerance; fertility; oxygen-effect; phytosanitary irradiation; radiotolerance ID FLY DIPTERA TEPHRITIDAE; IONIZING-RADIATION; FRUIT-FLY; PHYTOSANITARY TREATMENTS; GAMMA-IRRADIATION; INDUCED STERILITY; MOTH LEPIDOPTERA; NITROGEN; MALES; PUPAE AB Phytosanitary irradiation (PI) is increasingly being used for disinfesting fresh commodities from insect pests of quarantine concern. The development of generic doses of irradiation that can be applied to broadly control all pests within a particular family, order, or even all insects across commodities and packaging types could facilitate greater use of PI. Many commodities are stored in controlled or modified atmospheres that are low in oxygen to preserve commodity quality and extend shelf life, but low-oxygen environments have been shown to affect radiotolerance in some insects and more work is needed to understand the impact of this oxygen-effect on radiotolerance for both the development of generic doses and to increase the acceptance of irradiation as a treatment by the fresh commodity industry. Here we show that irradiation of cabbage looper, Trichoplusia ni (Hubner) (Lepidoptera: Noctuidae), in anoxic atmospheres (0 kPa aPO(2)) increases radiotolerance compared to irradiation in normoxic atmospheres (21 kPa aPO(2)) last instar larvae, true pupae and mature pupae (pharate adults) when considering the emergence of healthy-looking, viable adults as the critical metric. Of the 3 stages irradiated, the last instar larvae were most susceptible to irradiation, followed by pupae, with pharate adults 24-48 h prior to emergence (sometimes referred to as late pupae) being the most tolerant stage. When pharate adults were irradiated in anoxia, healthy-looking adults emerged at absorbed doses of 784-789 Gy whereas no healthy-looking adults emerged at the same doses in normoxia. Effects of anoxia on reproduction of irradiated female pharate adults were subtle. A few F-1 larvae hatched at doses estimated to be 585-591 Gy, suggesting that more work is needed to determine whether 400 Gy is an adequate generic dose to control late pupae of T. ni. C1 [Lopez-Martinez, Giancarlo; Hahn, Daniel A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Lopez-Martinez, Giancarlo] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Meagher, Robert L.] USDA ARS, CMAVF, Gainesville, FL 32608 USA. [Jeffers, Laura A.] USDA, APHIS, PPQ Ctr Plant Hlth Sci & Technol, Raleigh, NC USA. [Bailey, Woodward D.] USDA, APHIS, PPQ Subtrop Quarantine Inspect, Coral Gables, FL USA. RP Lopez-Martinez, G (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA.; Lopez-Martinez, G (reprint author), New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. EM gclopez@nmsu.edu FU FAO/IAEA Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments [D62008]; USDA-APHIS [13-8130-0159-CA]; Florida Agricultural Experiment Station FX This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. The research was funded by USDA-APHIS 13-8130-0159-CA to DAH and supported by the Florida Agricultural Experiment Station. The authors wish to thank Carl Gillis from the Florida Department of Agriculture and Consumer Services (FDACS-DPI) for providing us irradiation assistance and Andrea Beam USDA-APHIS-Miami and Catriona Condon UFL for help with alanine dosimetry. We thank Amy Rowley for rearing assistance and advice on the construction of the mating cages, Lyle Buss for taking photographs of our treated moths, and Sabrina White and Theodore Cogley for help in setup, husbandry of treated animals, and data collection. An IAEA/FAO Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments supported this work intellectually. Our research has benefited greatly from advice and support from members of the above group, and additional comments on this manuscript from Andrew Parker and Guy Hallman were most appreciated. NR 32 TC 1 Z9 1 U1 2 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 24 EP 33 PG 10 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700004 ER PT J AU Hallman, GJ Chapa, DL AF Hallman, Guy J. Chapa, Deanna L. TI Phytosanitary Irradiation of Diaphorina citri (Hemiptera: Liviidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE citrus greening disease; huanglongbing; acute lethal dose; prevent disease transmission; radiotolerance; sterilizing dose ID DISEASE AB Asian citrus psyllid (Diaphorina citri Kuwayama; Hemiptera: Liviidae) adults reared on orange jasmine (Murraya paniculata (L.) Jack; Sapindales: Rutaceae) were evaluated for acute mortality and prevention of reproduction when adult insects were irradiated for phytosanitary purposes. At 2 kGy all adults (n = 60) were dead by 72 h post irradiation. A dose of 150 Gy did not prevent oviposition (n = 1,200 adults), but no nymphs were observed to emerge from the eggs. At the next lowest dose (100 Gy) some nymphs developed. These results indicate that a minimum PI treatment dose significantly greater than 1.5 kGy but less than 2 kGy is required for total mortality within 72 h and the minimum PI treatment dose to prevent egg hatch is greater than 100 Gy, but less than 150 Gy. C1 [Hallman, Guy J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. [Chapa, Deanna L.] ARS, USDA, Anim Parasit Dis Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Hallman, GJ (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM G.J.Hallman@iaea.org FU FAO/IAEA Coordinated Research Project [D62008] FX Maura Rodriguez, Roberto Rivas, Faith Banda, Elvia Landero, Yadira Blanco and Marc Villalobos formerly of USDA-ARS, Weslaco, Texas are thanked for technical help. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 14 TC 3 Z9 3 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 150 EP 152 PG 3 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700024 ER PT J AU Hallman, GJ AF Hallman, Guy J. TI Phytosanitary irradiation of the invasive herbivorous terrestrial snail Cornu aspersum (Stylommatophora: Helicidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE escargot; invasive species; prevention of reproduction; radiosensitivity; regulated articles ID AUSTRALORBIS GLABRATUS; POPULATION KINETICS; IONIZING-RADIATION; EXPOSURE; AGE AB Phytosanitary measures are needed to prevent the transport of snails and slugs during trade in regulated articles. Ionizing radiation is increasing in use as a phytosanitary treatment against insect pests. Irradiation has the potential to be a treatment against snails and slugs, but little research has been done on radiosensitivity of terrestrial herbivorous gastropods. Adult Cornu aspersum (Muller) (Stylommatophora: Helicidae) were subjected to ionizing radiation at 25-100 Gy. Irradiation decreased the number of eggs laid, and those laid by adults irradiated with >= 75 Gy did not hatch. This research suggests that gastropod reproduction can be prevented with relatively low doses of radiation. A phytosanitary irradiation dose for C. aspersum should also serve to allow export of live snail species as food to areas that quarantine against them. C1 [Hallman, Guy J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. [Hallman, Guy J.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. RP Hallman, GJ (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Stored Prod Insect Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA.; Hallman, GJ (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. EM G.J.Hallman@iaea.org FU FAO/IAEA Coordinated Research Project [D62008] FX Beth Grafton-Cardwell and Janet McLain, Univ. of California, Kearney, are thanked for providing C. aspersum. Crissy Gutierrez and Maura Rodriguez, formerly with USDA-ARS, Weslaco, TX, are thanked for technical support. John Diaz-Montano, USDA-ARS, Manhattan, KS, is thanked for correcting the resumen. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 15 TC 1 Z9 1 U1 5 U2 5 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 156 EP 158 PG 3 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700026 ER PT J AU Hallman, GJ AF Hallman, Guy J. TI Phytosanitary Irradiation of Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE radiation; quarantine treatment; tobacco budworm; corn earworm ID IONIZING-RADIATION; QUARANTINE TREATMENT; TORTRICIDAE; DIPTERA; AMBIENT; LARVAE; EGGS AB Phytosanitary irradiation is being increasingly used as a phytosanitary treatment to disinfest fresh commodities of invasive species. Optimum irradiation treatment doses for a number of invasive species groups, such as the family Noctuidae, are required. This research was designed to provide data for developing an optimum generic irradiation dose suitable against all eggs and larvae of the Noctuidae. Last instars (the most radiotolerant stage normally found on exported commodities) of 2 noctuid species were studied, i.e., Heliothis virescens (F.) and Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae). There was no significant difference in emergence of normal-looking adults from H. virescens last instars reared on either a natural or an artificial host, i.e., mature pods of Phaseolus vulgaris L. (Fabales: Fabaceae) or a meridic diet, respectively. Two laboratory colonies of H. virescens were studied, one appeared to be more radiotolerant than the other and it was used for large-scale confirmatory testing of a putative phytosanitary irradiation dose treatment. A total of 14,366 last instars were irradiated with 150 Gy (actual measured dose distribution was 145-166 Gy) with none emerging as normal-looking adults. A total of 2,197 last instar H. zea reared on meridic diet were irradiated with the same dose and no normal-looking adults emerged. This research indicates that a treatment dose of approximately 150 Gy is sufficient as a phytosanitary treatment for these 2 noctuid species and adds to the body of research evidence that a generic dose treatment of 250 Gy is adequate to control eggs and larvae of Lepidoptera that infest fresh commodities. C1 [Hallman, Guy J.] ARS, USDA, Weslaco, TX 78596 USA. [Hallman, Guy J.] Vienna Int Ctr, Joint FAO IAEA Programme, Nucl Tech Food & Agr, Vienna, Austria. RP Hallman, GJ (reprint author), ARS, USDA, Weslaco, TX 78596 USA.; Hallman, GJ (reprint author), Vienna Int Ctr, Joint FAO IAEA Programme, Nucl Tech Food & Agr, Vienna, Austria. EM G.J.Hallman@iaea.org FU FAO/IAEA [D62008] FX Carlos Blanco, USDA-APHIS, is thanked for providing insects when he was formerly employed by USDA-ARS in Stoneville, Mississippi, USA. This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 22 TC 2 Z9 2 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 178 EP 181 PG 4 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700030 ER PT J AU Hallman, GJ Legaspi, JC Darmawi AF Hallman, Guy J. Legaspi, Jesusa Crisostomo Darmawi TI Phytosanitary irradiation of Diatraea saccharalis, D. grandiosella, and Eoreuma loftini (Lepidoptera: Crambidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE generic treatment dose; ionizing radiation; quarantine treatment; invasive species; phytosanitation; Diaprepes abbreviatus ID GY AB Phytosanitary irradiation (PI) is increasingly being used to disinfest horticultural commodities of invasive quarantine pests. Most disinfestation is done with generic treatments, where the same dose is applied against a group of pest species and/or commodities. The current generic treatment used by the Animal and Plant Health Inspection Service of the United States Department of Agriculture (USDA-APHIS) against Class Insecta-i.e., 400 Gy-does not include pupae of any species of Lepidoptera. More data are needed to develop a generic dose for those Lepidoptera that pupate on or within shipped commodities, such as Crambidae species. Tests were done with the crambids Diatraea grandiosella Dyar, D. saccharalis (F.), and Eoreuma loftini (Dyar) (Lepidoptera: Crambidae) to seek doses that would provide quarantine security no only against late pupae but also against last instar larvae, and that would possibly contribute to the development of a generic PI dose that is less than the current 400 Gy. The results of this research support proposed generic doses of 250 and 400 Gy for larvae and pupae of Lepidoptera species, respectively, and suggest lower specific doses for the individual insect species. C1 [Hallman, Guy J.] IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. [Legaspi, Jesusa Crisostomo] Florida A&M Univ, USDA, ARS, CMAVE,Ctr Biol Control, 6383 Mahan Dr, Tallahassee, FL 32608 USA. [Darmawi] CAIR BATAN, Jakarta, Indonesia. RP Hallman, GJ (reprint author), IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. EM g.j.hallman@iaea.org FU FAO/IAEA Coordinated Research Project on Development of Generic Irradiation Doses for Quarantine Treatments [D62008] FX This work was part of the FAO/IAEA Coordinated Research Project D62008 on Development of Generic Irradiation Doses for Quarantine Treatments. NR 15 TC 0 Z9 0 U1 0 U2 0 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD OCT PY 2016 VL 99 SI 2 BP 182 EP 185 PG 4 WC Entomology SC Entomology GA EF2HF UT WOS:000390144700031 ER PT J AU Marshall, CM Tartaglio, V Duarte, M Harmon, FG AF Marshall, Carine M. Tartaglio, Virginia Duarte, Maritza Harmon, Frank G. TI The Arabidopsis sickle Mutant Exhibits Altered Circadian Clock Responses to Cool Temperatures and Temperature-Dependent Alternative Splicing SO PLANT CELL LA English DT Article ID MESSENGER-RNA DECAY; TRANSCRIPTION FACTOR; HIGH-THROUGHPUT; PLANT; PROTEIN; GENES; THALIANA; NONSENSE; EXPRESSION; RHYTHMS AB The circadian clock allows plants to anticipate and respond to daily changes in ambient temperature. Mechanisms establishing the timing of circadian rhythms in Arabidopsis thaliana through temperature entrainment remain unclear. Also incompletely understood is the temperature compensation mechanism that maintains consistent period length within a range of ambient temperatures. A genetic screen for Arabidopsis mutants affecting temperature regulation of the PSEUDO-RESPONSE REGULATOR7 promoter yielded a novel allele of the SICKLE (SIC) gene. This mutant, sic-3, and the existing sic-1 mutant both exhibit low-amplitude or arrhythmic expression of core circadian clock genes under cool ambient temperature cycles, but not under light-dark entrainment. sic mutants also lengthen free running period in a manner consistent with impaired temperature compensation. sic mutant alleles accumulate LATE ELONGATED HYPOCOTYL (LHY) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1) splice variants, among other alternatively spliced transcripts, which is exacerbated by cool temperatures. The cca1-1 lhy-20 double mutant is epistatic to sic-3, indicating the LHY and CCA1 splice variants are needed for sic-3 circadian clock phenotypes. It is not expected that SIC is directly involved in the circadian clock mechanism; instead, SIC likely contributes to pre-mRNA metabolism, and the splice variants that accumulate in sic mutants likely affect the circadian clock response to cool ambient temperature. C1 [Marshall, Carine M.; Tartaglio, Virginia; Duarte, Maritza; Harmon, Frank G.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Marshall, Carine M.; Tartaglio, Virginia; Duarte, Maritza; Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Harmon, FG (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA.; Harmon, FG (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM fharmon@berkeley.edu OI Marshall, Carine/0000-0001-7178-4664 FU USDA [2030-21000-039-00D]; National Science Foundation [IOS1238048]; University of California, Berkeley FX We are thank C. Robertson McClung (Dartmouth College) for the ProPRR7: LUC reporter line, Bryan Thines (University of California, Berkeley) for creating the EMS-mutagenized population, Michael Covington (University of California, Davis) for sharing his polar plot script for R, and Michael Koontz (University of California, Davis) for help modifying the polar plot script. We thank Riva Bruenn (University of California, Berkeley) for her advice and experience in phylogenetic analysis. We thank Mark Mullan, Cody Schaaf, and Samantha Nguyen for research assistance. We thank University of California, Berkeley Summer Undergraduate Fellowships/Rose Hills for supporting Mark Mullan. We thank Claire Bendix, Emma Kovak, Dominica Rohozinski, Dominik Brilhaus, and Achim Werner for valuable feedback on early drafts of the manuscript. This work is supported by the USDA (2030-21000-039-00D to F.G.H.) and by the National Science Foundation (IOS1238048 to F.G.H.). NR 89 TC 3 Z9 3 U1 7 U2 7 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2560 EP 2575 DI 10.1105/tpc.16.00223 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400019 PM 27624757 ER PT J AU Kumar, S Kempinski, C Zhuang, X Norris, A Mafu, S Zi, JC Bell, SA Nybo, SE Kinison, SE Jiang, ZD Goklany, S Linscott, KB Chen, XL Jia, QD Brown, SD Bowman, JL Babbitt, PC Peters, RJ Chen, F Chappell, J AF Kumar, Santosh Kempinski, Chase Zhuang, Xun Norris, Ayla Mafu, Sibongile Zi, Jiachen Bell, Stephen A. Nybo, Stephen Eric Kinison, Scott E. Jiang, Zuodong Goklany, Sheba Linscott, Kristin B. Chen, Xinlu Jia, Qidong Brown, Shoshana D. Bowman, John L. Babbitt, Patricia C. Peters, Reuben J. Chen, Feng Chappell, Joe TI Molecular Diversity of Terpene Synthases in the Liverwort Marchantia polymorpha SO PLANT CELL LA English DT Article ID ENT-KAURENE SYNTHASE; MOSS PHYSCOMITRELLA-PATENS; BIFUNCTIONAL DITERPENE CYCLASE; COPALYL DIPHOSPHATE SYNTHASE; FUNCTION LINKAGE DATABASE; PRE-STEADY-STATE; LAND PLANTS; FUNCTIONAL-CHARACTERIZATION; GIBBERELLIN BIOSYNTHESIS; NATURAL-PRODUCTS AB Marchantia polymorpha is a basal terrestrial land plant, which like most liverworts accumulates structurally diverse terpenes believed to serve in deterring disease and herbivory. Previous studies have suggested that the mevalonate and methylerythritol phosphate pathways, present in evolutionarily diverged plants, are also operative in liverworts. However, the genes and enzymes responsible for the chemical diversity of terpenes have yet to be described. In this study, we resorted to a HMMER search tool to identify 17 putative terpene synthase genes from M. polymorpha transcriptomes. Functional characterization identified four diterpene synthase genes phylogenetically related to those found in diverged plants and nine rather unusual monoterpene and sesquiterpene synthase-like genes. The presence of separate monofunctional diterpene synthases for ent-copalyl diphosphate and ent-kaurene biosynthesis is similar to orthologs found in vascular plants, pushing the date of the underlying gene duplication and neofunctionalization of the ancestral diterpene synthase gene family to >400 million years ago. By contrast, the mono-and sesquiterpene synthases represent a distinct class of enzymes, not related to previously described plant terpene synthases and only distantly so to microbial-type terpene synthases. The absence of a Mg2+ binding, aspartate-rich, DDXXD motif places these enzymes in a noncanonical family of terpene synthases. C1 [Kumar, Santosh; Kempinski, Chase; Zhuang, Xun; Bell, Stephen A.; Nybo, Stephen Eric; Kinison, Scott E.; Jiang, Zuodong; Goklany, Sheba; Linscott, Kristin B.; Chappell, Joe] Univ Kentucky, Plant Biol Program, Lexington, KY 40536 USA. [Kumar, Santosh; Kempinski, Chase; Zhuang, Xun; Bell, Stephen A.; Nybo, Stephen Eric; Kinison, Scott E.; Jiang, Zuodong; Goklany, Sheba; Linscott, Kristin B.; Chappell, Joe] Univ Kentucky, Dept Pharmaceut Sci, Lexington, KY 40536 USA. [Norris, Ayla; Jia, Qidong; Chen, Feng] Univ Tennessee, Gradaute Sch Genome Sci & Technol, Knoxville, TN 37996 USA. [Mafu, Sibongile; Zi, Jiachen; Peters, Reuben J.] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Chen, Xinlu; Chen, Feng] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Brown, Shoshana D.; Babbitt, Patricia C.] Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Bioengn, San Francisco, CA 94158 USA. [Brown, Shoshana D.; Babbitt, Patricia C.] Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Therapeut Sci & Pharmaceut Chem, San Francisco, CA 94158 USA. [Bowman, John L.] Monash Univ, Sch Biol Sci, Melbourne, Vic 3800, Australia. [Zhuang, Xun] Sandia Natl Labs, Livermore, CA 94551 USA. [Norris, Ayla] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Mafu, Sibongile] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Zi, Jiachen] Jinan Univ, Biotechnol Inst Chinese Mat Med, Guangzhou 510632, Guangdong, Peoples R China. [Bell, Stephen A.] Univ Utah, Dept Med Chem, Salt Lake City, UT 84112 USA. [Nybo, Stephen Eric] Ferris State Univ, Med Chem, Coll Pharm, Big Rapids, MI 49307 USA. [Goklany, Sheba] Arizona State Univ, Sch Engn Matter Transport & Energy, Tempe, AZ 85287 USA. RP Chappell, J (reprint author), Univ Kentucky, Plant Biol Program, Lexington, KY 40536 USA.; Chappell, J (reprint author), Univ Kentucky, Dept Pharmaceut Sci, Lexington, KY 40536 USA. EM chappell@uky.edu RI Peters, Reuben/D-1196-2010; OI Peters, Reuben/0000-0003-4691-8477; Chen, Xinlu/0000-0002-7560-6125 FU National Institutes of Health [1RC2GM092521, GM076324, GM60595]; University of Tennessee Institute of Agriculture; Australian Research Council [DP160100892]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX We thank Tobias G. Kollner and Jonathan Gershenzon for their contributions in the early stage of this study. This work was supported, in part, by Grants 1RC2GM092521 from the National Institutes of Health to JC, GM076324 from the National Institutes of Health to RJP, an Innovation Grant from the University of Tennessee Institute of Agriculture to F.C., GM60595 from the National Institutes of Health to P.C.B., and DP160100892 from the Australian Research Council to J.L.B. We also thank our colleagues at Joint Genome Institute for access to the M. polymorpha genome sequence information, work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, supported by the Office of Science of the U.S. Department of Energy under Contract DE-AC02-05CH11231. NR 88 TC 0 Z9 0 U1 10 U2 10 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2632 EP 2650 DI 10.1105/tpc.16.00062 PG 19 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400024 PM 27650333 ER PT J AU Richter, A Schaff, C Zhang, ZW Lipka, AE Tian, F Kollner, TG Schnee, C Preiss, S Irmisch, S Jander, G Boland, W Gershenzon, J Buckler, ES Degenhardt, J AF Richter, Annett Schaff, Claudia Zhang, Zhiwu Lipka, Alexander E. Tian, Feng Kollner, Tobias G. Schnee, Christiane Preiss, Susanne Irmisch, Sandra Jander, Georg Boland, Willhelm Gershenzon, Jonathan Buckler, Edward S. Degenhardt, Jorg TI Characterization of Biosynthetic Pathways for the Production of the Volatile Homoterpenes DMNT and TMTT in Zea mays SO PLANT CELL LA English DT Article ID ASSOCIATION MAPPING POPULATION; GENOME-WIDE ASSOCIATION; INDUCED PLANT VOLATILES; ISOPRENOID BIOSYNTHESIS; FARNESYL DIPHOSPHATE; GENETIC ARCHITECTURE; MEDICAGO-TRUNCATULA; HERBIVORE ENEMIES; CHEMICAL ECOLOGY; INDIRECT DEFENSE AB Plant volatiles not only have multiple defense functions against herbivores, fungi, and bacteria, but also have been implicated in signaling within the plant and toward other organisms. Elucidating the function of individual plant volatiles will require more knowledge of their biosynthesis and regulation in response to external stimuli. By exploiting the variation of herbivore-induced volatiles among 26 maize (Zea mays) inbred lines, we conducted a nested association mapping and genome-wide association study (GWAS) to identify a set of quantitative trait loci (QTLs) for investigating the pathways of volatile terpene production. The most significant identified QTL affects the emission of (E)-nerolidol, linalool, and the two homoterpenes (E)-3,8-dimethyl-1,4,7-nonatriene (DMNT) and (E, E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). GWAS associated a single nucleotide polymorphism in the promoter of the gene encoding the terpene synthase TPS2 with this QTL. Biochemical characterization of TPS2 verified that this plastid-localized enzyme forms linalool, (E)-nerolidol, and (E, E)-geranyllinalool. The subsequent conversion of (E)-nerolidol into DMNT maps to a P450 monooxygenase, CYP92C5, which is capable of converting nerolidol into DMNT by oxidative degradation. A QTL influencing TMTT accumulation corresponds to a similar monooxygenase, CYP92C6, which is specific for the conversion of (E, E)-geranyllinalool to TMTT. The DMNT biosynthetic pathway and both monooxygenases are distinct from those previously characterized for DMNT and TMTT synthesis in Arabidopsis thaliana, suggesting independent evolution of these enzymatic activities. C1 [Richter, Annett; Schaff, Claudia; Preiss, Susanne; Degenhardt, Jorg] Martin Luther Univ Halle Wittenberg, Inst Pharm, D-06120 Halle, Germany. [Zhang, Zhiwu; Lipka, Alexander E.; Tian, Feng; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Kollner, Tobias G.; Schnee, Christiane; Irmisch, Sandra; Boland, Willhelm; Gershenzon, Jonathan] Max Planck Inst Chem Ecol, D-07745 Jena, Germany. [Richter, Annett; Jander, Georg] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. [Zhang, Zhiwu] Washington State Univ, Dept Crops & Soil Sci, Pullman, WA 99164 USA. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Tian, Feng] China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100193, Peoples R China. [Irmisch, Sandra] Univ British Columbia, Vancouver, BC V6T 1Z4, Canada. RP Degenhardt, J (reprint author), Martin Luther Univ Halle Wittenberg, Inst Pharm, D-06120 Halle, Germany. EM joerg.degenhardt@pharmazie.uni-halle.de RI Gershenzon, Jonathan/K-1331-2013; Boland, Wilhelm/K-7762-2012; OI Gershenzon, Jonathan/0000-0002-1812-1551; Kollner, Tobias/0000-0002-7037-904X FU Collaborative Research Center 648 of the German Research Foundation (DFG); U.S. National Science Foundation [IOS-0820619, IOS-1238014, IOS-1139329, IOS-1339237]; USDA-Agricultural Research Service FX Wethank Jens Wurlitzer for plant collection, Michael Gore for processing of samples from the field, and Peter Lindemann for help with enzyme characterization. Wealso thank KWS Seeds for maize B73 seeds. A.R. and J.D. were supported by project B7 of the Collaborative Research Center 648 of the German Research Foundation (DFG). Germplasm, field, and mapping analysis were supported by grants from the U.S. National Science Foundation (IOS-0820619 and IOS-1238014) and the USDA-Agricultural Research Service. G.J. was supported by U.S. National Science Foundation Awards IOS-1139329 and IOS-1339237. NR 78 TC 1 Z9 1 U1 9 U2 9 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD OCT PY 2016 VL 28 IS 10 BP 2651 EP 2665 DI 10.1105/tpc.15.00919 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA EF2DR UT WOS:000390135400025 PM 27662898 ER PT J AU Djanibekov, U Villamor, GB Dzhakypbekova, K Chamberlain, J Xu, JC AF Djanibekov, Utkur Villamor, Grace B. Dzhakypbekova, Klara Chamberlain, James Xu, Jianchu TI Adoption of Sustainable Land Uses in Post-Soviet Central Asia: The Case for Agroforestry SO SUSTAINABILITY LA English DT Article DE agroforestry; coevolution; sustainable land use; socio-ecological systems; transition countries ID AMU-DARYA; CLIMATE-CHANGE; UZBEKISTAN; SYSTEMS; PRODUCTIVITY; AGRICULTURE; PERFORMANCE; GOVERNANCE; SERVICES; IMPACTS AB We examine constraints and opportunities to enhance adoption of agroforestry for ecosystem and livelihood improvement in post-Soviet economies, using Central Asian countries as examples. Using a coevolutionary socio-ecological systems framework, we describe how development efforts, especially agricultural policies, under centrally planned regimes and under transition to market economies have changed environmental conditions, and how they affect peoples' welfare. We then discuss agroforestry as a sustainable land use practice to address these issues. We present regional-specific and -suitable agroforestry practices, and discuss their potential. We found that legacies from the previous period of central planning shape current land uses, institutions, infrastructure and decisions of farmers, and constrain development of agroforestry. By identifying opportunities and constraints of agroforestry, we provide recommendations for enhancing the use of agroforestry in Central Asia. C1 [Djanibekov, Utkur] Univ Bonn, Inst Food & Resource Econ ILR, Meckenheimer Allee 174, D-53115 Bonn, Germany. [Villamor, Grace B.] Univ Bonn, Ctr Dev Res ZEF, Walter Flex Str 3, D-53113 Bonn, Germany. [Dzhakypbekova, Klara] Univ Bonn, Agr Sci & Resource Management Trop & Subtrop, Nussallee 1, D-53115 Bonn, Germany. [Chamberlain, James] US Forest Serv, Southern Res Stn, FIA, 1710 Res Ctr Dr, Blacksburg, VA 24060 USA. [Xu, Jianchu] Kunming Inst Bot, World Agroforestry Ctr East & Cent Asia, Kunming 650201, Peoples R China. RP Djanibekov, U (reprint author), Univ Bonn, Inst Food & Resource Econ ILR, Meckenheimer Allee 174, D-53115 Bonn, Germany. EM u.djanibekov@ilr.uni-bonn.de; gracev@uni-bonn.de; klara.dzhakypbekova@uni-bonn.de; jchamberlain@fs.fed.us; j.c.xu@cgiar.org NR 94 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD OCT PY 2016 VL 8 IS 10 AR 1030 DI 10.3390/su8101030 PG 16 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA EE1BE UT WOS:000389314600070 ER PT J AU Tani, M Horvath, KV Lamarche, B Couture, P Burnett, JR Schaefer, EJ Asztalos, BF AF Tani, Mariko Horvath, Katalin V. Lamarche, Benoit Couture, Patrick Burnett, John R. Schaefer, Ernst J. Asztalos, Bela F. TI High-density lipoprotein subpopulation profiles in lipoprotein lipase and hepatic lipase deficiency SO ATHEROSCLEROSIS LA English DT Article DE Apolipoproteins; Lipoprotein metabolism; HDL particles; HDL remodeling; Reverse cholesterol transport ID APOLIPOPROTEIN-A-I; CORONARY-ARTERY-DISEASE; HDL SUBPOPULATIONS; APOA-I; ENDOTHELIAL LIPASE; HEART-DISEASE; METABOLISM; HUMANS; PLASMA; CHOLESTEROL AB Background and aims: Our aim was to gain insight into the role that lipoprotein lipase (LPL) and hepatic lipase (HL) plays in HDL metabolism and to better understand LPL- and HL-deficiency states. Methods: We examined the apolipoprotein (apo) A-I-, A-II-, A-IV-, C-I-, C-III-, and E-containing HDL subpopulation profiles, assessed by native 2-dimensional gel-electrophoresis and immunoblotting, in 6 homozygous and 11 heterozygous LPL- deficient, 6 homozygous and 4 heterozygous HL-deficient, and 50 control subjects. Results: LPL- deficient homozygotes had marked hypertriglyceridemia and significant decreases in LDL-C, HDL-C, and apoA-I. Their apoA-I-containing HDL subpopulation profile was shifted toward small HDL particles compared to controls. HL-deficient homozygotes had moderate hypertriglyceridemia, modest increases in LDL-C and HDL-C level, but normal apoA-I concentration. HL-deficient homozygotes had a unique distribution of apoA-I-containing HDL particles. The normally apoA-I: A-II, intermediate-size (alpha-2 and alpha-3) particles were significantly decreased, while the normally apoA-I only ( very large alpha-1, small alpha-4, and very small pre beta-1) particles were significantly elevated. In contrast to control subjects, the very large alpha-1 particles of HL-deficient homozygotes were enriched in apoA-II. Homozygous LPL- and HL-deficient subjects also had abnormal distributions of apo C-I, C-III, and E in HDL particles. Values for all measured parameters in LPL- and HL-deficient heterozygotes were closer to values measured in controls than in homozygotes. Conclusions: Our data are consistent with the concept that LPL is important for the maturation of small discoidal HDL particles into large spherical HDL particles, while HL is important for HDL remodeling of very large HDL particles into intermediate-size HDL particles. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Tani, Mariko; Horvath, Katalin V.; Schaefer, Ernst J.; Asztalos, Bela F.] Tufts Univ, Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Lamarche, Benoit; Couture, Patrick] Univ Laval, Inst Nutraceut & Funct Foods, Quebec City, PQ, Canada. [Burnett, John R.] Univ Western Australia, Royal Perth & Fiona Stanley Hosp Network, Path West Lab Med, Dept Clin Biochem, Perth, WA, Australia. [Burnett, John R.] Univ Western Australia, Sch Med & Pharmacol, Perth, WA, Australia. RP Asztalos, BF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr, Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM bela.asztalos@tufts.edu FU National Heart, Lung, and Blood Institute [HL117933]; US Department of Agriculture [53-3K06-5-10]; Royal Perth Hospital Medical Research Foundation FX This research was supported by grant (HL117933) from the National Heart, Lung, and Blood Institute to BFA and by contract (53-3K06-5-10) from the US Department of Agriculture to EJS. JRB was supported by a Practitioner Fellowship from the Royal Perth Hospital Medical Research Foundation. NR 38 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD OCT PY 2016 VL 253 BP 7 EP 14 DI 10.1016/j.atherosclerosis.2016.08.014 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA EE2GG UT WOS:000389401200003 PM 27573733 ER PT J AU Yubero-Serrano, EM Delgado-Lista, J Alcala-Diaz, JF Garcia-Rios, A Perez-Caballero, AI Blanco-Rojo, R Gomez-Delgado, F Marin, C Tinahones, FJ Caballero, J Ordovas, JM van Ommen, B Perez-Jimenez, F Perez-Martinez, P Lopez-Miranda, J AF Yubero-Serrano, Elena M. Delgado-Lista, Javier Alcala-Diaz, Juan F. Garcia-Rios, Antonio Perez-Caballero, Ana I. Blanco-Rojo, Ruth Gomez-Delgado, Francisco Marin, Carmen Tinahones, Francisco J. Caballero, Javier Ordovas, Jose M. van Ommen, Ben Perez-Jimenez, Francisco Perez-Martinez, Pablo Lopez-Miranda, Jose TI A dysregulation of glucose metabolism control is associated with carotid atherosclerosis in patients with coronary heart disease (CORDIOPREV-DIAB study) SO ATHEROSCLEROSIS LA English DT Article DE Coronary heart disease; Intima media thickness; Glycemic control; OGTT; Posprandial glycemia; Age ID INTIMA-MEDIA THICKNESS; PRIOR MYOCARDIAL-INFARCTION; FASTING PLASMA-GLUCOSE; CARDIOVASCULAR-DISEASE; INSULIN-RESISTANCE; ARTERY INTIMA; SUBCLINICAL ATHEROSCLEROSIS; GLYCEMIC CONTROL; RISK-FACTORS; LIFE-STYLE AB Background and aims: Patients with coexisting coronary heart disease (CHD) and type 2 diabetes mellitus (T2DM) are at high risk of cardiovascular recurrence, however, it is not well established whether they exhibit an increased intima-media thickness of both common carotid arteries (IMT-CC). Furthermore, whether this relationship is inherent to T2DM or depends on glycemic control has not been tested in large cohorts. Our aim was to determine whether clinical categories and/or analytical markers of glucose metabolism control were associated with IMT-CC in CHD patients. Methods: 1002 patients aged 20-75 years, categorized into normal glucose tolerance (NGT), impaired fasting glucose (IFG), impaired glucose tolerance (IGT) or T2DM, underwent an oral glucose tolerance test (OGTT) and an IMT-CC measurement. Results: IMT-CC was higher in T2DM patients with HbA1c > 6.5% compared to T2DM patients with HbA1c < 6.5% (p - 0.001), patients with IFG or IGT (p < 0.001) and NGT (p < 0.001). When age was considered, IMT-CC was influenced by glucose metabolism control only in e patients with age < 61 years (p < 0.01). In a multiple linear regression analysis, glucose concentration at 120 min, but not other OGTT time-points appeared as a significant independent contributor of IMT-CC (p < 0.001). Moreover, a multiple logistic regression and the area under curve (AUC) of the ROC curve analysis showed a predictive power of glucose at 120 min to detect those CHD patients at the highest risk, defined as IMT-CC >= 0.7 mm (R-2 = 0.221; AUC = 0.761). Conclusions: Our results highlight the importance of properly controlling glucose metabolism in CHD patients, in younger populations in particular, providing an easy way of categorizing patients with an increased IMT-CC. Moreover, glucose concentration at 120 min could contribute to CVD risk and its determination could be used as a predictive tool to identify those CHD patients at the highest risk. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Yubero-Serrano, Elena M.; Delgado-Lista, Javier; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Perez-Caballero, Ana I.; Blanco-Rojo, Ruth; Gomez-Delgado, Francisco; Marin, Carmen; Tinahones, Francisco J.; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Univ Cordoba, Reina Sofia Univ Hosp, Maimonides Biomed Res Inst Cordoba IMIBIC, Lipids & Atherosclerosis Unit, E-14071 Cordoba, Spain. [Yubero-Serrano, Elena M.; Delgado-Lista, Javier; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Perez-Caballero, Ana I.; Blanco-Rojo, Ruth; Gomez-Delgado, Francisco; Marin, Carmen; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain. [Tinahones, Francisco J.] Univ Malaga, Virgen de la Victoria Hosp, Biomed Res Inst Malaga IBIMA, Malaga, Spain. [Caballero, Javier] Reina Sofia Univ Hosp, Clin Anal Serv, Cordoba, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [van Ommen, Ben] TNO, Zeist, Netherlands. RP Delgado-Lista, J; Lopez-Miranda, J (reprint author), Reina Sofia Univ Hosp, IMIBIC, Lipids & Atherosclerosis Res Unit, Avda Menendez Pidal S-N, Cordoba 14004, Spain. EM delgadolista@gmail.com; jlopezmir@uco.es RI Yubero-Serrano, Elena /H-4832-2013; OI Blanco-Rojo, Ruth/0000-0002-6159-3597; Gomez Delgado, Francisco/0000-0002-0216-2084 FU Fundacion Patrimonio Comunal Olivarero; Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa); Diputaciones de Jaen y Cordoba; Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Ministerio de Medio Ambiente, MedioRural y Marino, Gobierno de Espana; Consejeria de Salud, Junta de Andalucia [PI0193/09, PI-0252/09, PI-0058/10]; Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo [CVI-7450]; Merck Serono and Fundacion; Ministerio de Ciencia e Innovacion [AGL2009-122270]; Ministerio de Economia y Competitividad [AGL2012/39615, FIS PI10/01041, FIS PI13/00023]; Instituto de Salud Carlos III (ISCIII) of Spain; Directorate General for Assessment and Promotion of Research; EU's European Regional Development Fund (FEDER); European Community [289511]; ISCIII (Programa Rio-Hortega) FX The CordioPrev study is supported by the Fundacion Patrimonio Comunal Olivarero, by Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, MedioRural y Marino, Gobierno de Espana. The CordioPrev study also received research grants from Consejeria de Salud, Junta de Andalucia (PI0193/09 to J L-M, PI-0252/09 to J D-L, and PI-0058/10 to P P-M); Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo (CVI-7450 to J L-M); Merck Serono and Fundacion 2000 (Clinical research in Cardiometabolic to P P-M), and from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to J L-M); Ministerio de Economia y Competitividad (AGL2012/39615 to J L-M, FIS PI10/01041 to P P-M, FIS PI13/00023 to J D-L), integrated into the framework of the National Plan for Scientific Research, Technological Development and Innovation 2008-2011 and 2013-2016, co-financed by the Instituto de Salud Carlos III (ISCIII) of Spain and also by the Directorate General for Assessment and Promotion of Research and the EU's European Regional Development Fund (FEDER). It was also partly supported by a Research Grant from the European Community (NUTRITECH European Integrated Project-289511). F.G.D. is supported by a research contract of ISCIII (Programa Rio-Hortega). The CIBEROBN is an initiative of the ISCIII. The contents of this paper are not intended for any commercial use. NR 55 TC 1 Z9 1 U1 2 U2 2 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD OCT PY 2016 VL 253 BP 178 EP 185 DI 10.1016/j.atherosclerosis.2016.07.903 PG 8 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA EE2GG UT WOS:000389401200027 PM 27451413 ER PT J AU El-Sabaawi, RW Frauendorf, TC Marques, PS Mackenzie, RA Manna, LR Mazzoni, R Phillip, DAT Warbanski, ML Zandona, E AF El-Sabaawi, Rana W. Frauendorf, Therese C. Marques, Piata S. Mackenzie, Richard A. Manna, Luisa R. Mazzoni, Rosana Phillip, Dawn A. T. Warbanski, Misha L. Zandona, Eugenia TI Biodiversity and ecosystem risks arising from using guppies to control mosquitoes SO BIOLOGY LETTERS LA English DT Article DE dengue; ecosystem service; invasion; malaria; Zika ID LIFE-HISTORY EVOLUTION; POECILIA-RETICULATA; TRINIDADIAN GUPPIES; RIVULUS-HARTII; LARVAE; WATER; ADAPTATION; STREAMS; SPREAD; FISH AB Deploying mosquito predators such as the guppy (Poecilia reticulata) into bodies of water where mosquitoes breed is a common strategy for limiting the spread of disease-carrying mosquitoes. Here, we draw on studies from epidemiology, conservation, ecology and evolution to show that the evidence for the effectiveness of guppies in controlling mosquitoes is weak, that the chances of accidental guppy introduction into local ecosystems are large, and that guppies can easily establish populations and damage these aquatic ecosystems. We highlight several knowledge and implementation gaps, and urge that this approach is either abandoned in favour of more effective strategies or that it is used much more rigorously. Controlling mosquitoes does not need to come at the expense of freshwater biodiversity. C1 [El-Sabaawi, Rana W.; Frauendorf, Therese C.; Marques, Piata S.; Warbanski, Misha L.] Univ Victoria, Dept Biol, Stn CSC, POB 1700, Victoria, BC V8W 2Y2, Canada. [Mackenzie, Richard A.] USDA Forest Serv, Inst Pacific Isl, Pacific Southwest Res Stn, Hilo, HI 96720 USA. [Manna, Luisa R.; Mazzoni, Rosana; Zandona, Eugenia] Univ Estado Rio de Janeiro, Dept Ecol, Rua Sao Francisco Xavier 524, BR-20550900 Rio De Janeiro, Brazil. [Phillip, Dawn A. T.] Univ West Indies, Dept Life Sci, St Augustine, Trinid & Tobago. RP El-Sabaawi, RW (reprint author), Univ Victoria, Dept Biol, Stn CSC, POB 1700, Victoria, BC V8W 2Y2, Canada. EM rana@uvic.ca FU IDRC/AUCC; CNPq; Science without Borders (CAPES) fellowship; University of Victoria FX We have received no funding for this study. Past and ongoing work on guppies in our groups has been funded by a number of sources (IDRC/AUCC, CNPq, etc) that are acknowledged in the primary papers we cite in this article. PSM is supported by a Science without Borders (CAPES) fellowship. TCF is supported by funding from the University of Victoria. NR 44 TC 0 Z9 0 U1 14 U2 14 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1744-9561 EI 1744-957X J9 BIOL LETTERS JI Biol. Lett. PD OCT 1 PY 2016 VL 12 IS 10 AR 20160590 DI 10.1098/rsbl.2016.0590 PG 4 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA EE5HM UT WOS:000389636800011 ER PT J AU Gill, TA Li, J Saenger, M Scofield, SR AF Gill, T. A. Li, J. Saenger, M. Scofield, S. R. TI Thymol-based submicron emulsions exhibit antifungal activity against Fusarium graminearum and inhibit Fusarium head blight in wheat SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE antifungal; biocide; Fusarium graminearum; thymol; wheat ID ESSENTIAL OILS; ANTIMICROBIAL EFFICACY; DEOXYNIVALENOL ACCUMULATION; MYCOTOXIN PRODUCTION; MEMBRANE INTEGRITY; PHENOLIC-COMPOUNDS; F-GRAMINEARUM; FUNGI; NANOEMULSIONS; RESISTANCE AB AimsFusarium graminearum is a very destructive fungal pathogen that leads to Fusarium head blight (FHB) in wheat, a disease which costs growers millions of dollars annually both in crop losses and in remediation efforts. Current countermeasures include the deployment of wheat varieties with some resistance to FHB in conjunction with timed fungicide treatments. In this article, we introduce a fungicide based on thymol, a naturally occurring plant phenolic derived from essential oils. To overcome the hydrophobicity of thymol, the thymol active was incorporated into a low-surfactant submicron emulsion with and without a carrier oil. Methods and ResultsThe minimum fungicidal concentration of F.graminearum was found to be both 002% for thymol emulsions with and without an oil component. Time-to-kill experiments showed that thymol emulsions were able to inactivate F.graminearum in as little as 10s at concentrations above 006%. Spraying the thymol emulsions (similar to 01% range) on the wheat variety Bobwhite demonstrated significant reductions in FHB infection rate (number of infected spikelets). However, with 05% thymol, the wheat heads exhibited premature senescence. Transmission and scanning electron micrographs suggest that the mechanism of antifungal action is membrane mediated, as conidia exposed to thymol showed complete organelle disorganization and evidence of lipid emulsification. ConclusionThe collective experimental data suggest that thymol emulsions may be an effective naturally derived alternative to the current thymol treatments, and chemical fungicides in ameliorating FHB. Significance and Impact of the StudyThis is the first thymol-derived nanoemulsion particles resuspended into water and not DMSO, exhibiting the same antibacterial/antifungal activity as previously described thymol and thyme oil treatments. This drastically reduces the environmental footprint thymol will leave if utilized as a fungicide treatment on field crops. C1 [Gill, T. A.; Scofield, S. R.] Purdue Univ, USDA ARS, Coll Agr, Dept Agron, W Lafayette, IN 47907 USA. [Li, J.; Saenger, M.] Purdue Univ, Coll Vet Med, Dept Basic Med Sci, W Lafayette, IN 47907 USA. RP Gill, TA (reprint author), Univ Florida, Dept Entomol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM tagill@ufl.edu NR 47 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD OCT PY 2016 VL 121 IS 4 BP 1103 EP 1116 DI 10.1111/jam.13195 PG 14 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DW3XG UT WOS:000383575800019 PM 27253757 ER PT J AU Spurgeon, DW Brent, CS AF Spurgeon, D. W. Brent, C. S. TI Development, Survival, and Hatching Periodicity of Lygus hesperus (Hemiptera: Miridae) Eggs under Constant and Variable Temperatures SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Lygus hesperus; western tarnished plant bug; thermoperiod; development ID WATER-STRESS; CANOPY TEMPERATURE; COTTON; INDICATOR; NYMPHS; IRRIGATION; INSECTS; BUG AB The microclimate within western cotton (Gossypium spp.) seems favorable for the western tarnished plant bug (Lygus hesperus Knight) (Hemiptera: Miridae), provided adequate soil moisture is available. Diminishing water supplies and increasing costs in the West will likely change irrigation practices and induce at least periodically unfavorable conditions for L. hesperus. Knowledge of L. hesperus thermal ecology has been limited to constant temperatures for which relevance to variable temperature environments is unknown. Eggs of L. hesperus were reared under low (mean = 15 degrees C), medium (mean = 22 degrees C), or high (mean = 29 degrees C) constant (+/-<0.5 degrees C) or diurnally fluctuating (+/- 8 degrees C) temperatures. Developmental time and survival were similar between constant and variable regimes at the medium temperature. In contrast, variable low temperatures hastened egg development and increased survival, whereas variable high temperatures delayed development and reduced survival compared with constant regimes. Within the studied temperature range, the relationship between temperature and egg developmental rate was linear for constant temperatures, but a quadratic term was needed to describe this relationship under variable temperatures. Under medium variable temperatures, egg hatch was disproportionately high during the warmest period of the day (1300-1900 h) compared with the constant regime. Differences between regimes were less pronounced at high temperatures, except for the conspicuous absence of hatch between 1300 and 1900 h in the variable regime when temperatures were always >32 degrees C. These results indicate the limited utility of constant temperature data for understanding L. hesperus thermal ecology and provide baseline information to better plan and interpret applied studies of L. hesperus thermal ecology. C1 [Spurgeon, D. W.; Brent, C. S.] ARS, USDA, Pest Management & Biocontrol Res Unit, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. RP Spurgeon, DW (reprint author), ARS, USDA, Pest Management & Biocontrol Res Unit, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM dale.spurgeon@ars.usda.gov NR 35 TC 0 Z9 0 U1 5 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD OCT PY 2016 VL 51 IS 4 BP 292 EP 304 PG 13 WC Entomology SC Entomology GA EE4GT UT WOS:000389561000003 ER PT J AU Suh, CPC Spurgeon, DW AF Suh, C. P. -C. Spurgeon, D. W. TI Continued Pheromone Release by Boll Weevils (Coleoptera: Curculionidae) Following Host Removal SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE boll weevil; pheromone; traps; cotton; food removal C1 [Suh, C. P. -C.; Spurgeon, D. W.] USDA ARS, Insect Control & Cotton Dis Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. [Spurgeon, D. W.] USDA ARS, Pest Management & Biocontrol Res Unit, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. RP Suh, CPC (reprint author), USDA ARS, Insect Control & Cotton Dis Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. EM charles.suh@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD OCT PY 2016 VL 51 IS 4 BP 332 EP 335 PG 4 WC Entomology SC Entomology GA EE4GT UT WOS:000389561000009 ER PT J AU Petersen, MK Muscha, JM Mulliniks, JT Roberts, AJ AF Petersen, M. K. Muscha, J. M. Mulliniks, J. T. Roberts, A. J. TI Water temperature impacts water consumption by range cattle in winter SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE in vitro; neutral detergent fiber disappearance; range cows; rumen temperature; water intake; water temperature ID FEED-INTAKE; COWS; RUMEN; SHEEP; SNOW AB Water consumption and DMI have been found to be positively correlated, and both may interact with ingestion of cold water or grazed frozen forage due to transitory reductions in the temperature of ruminal contents. The hypothesis underpinning the study explores the potential that cows provided warm drinking water would have increased in situ NDF and OM disappearances and a more stable rumen temperature, drink more water, and lose less BW during the winter. This hypothesis was tested in 3 experiments. In Exp. 1, ruminal extrusa (93.1% DM, 90.2% OM, 81.1% NDF [DM], and 4.9% CP [DM]) were randomly allocated to 1 of 5 in vitro incubation temperatures. In 2 independent trials, temperatures evaluated were 39, 37, or 35 degrees C (trial 1) and 39, 33, or 31 degrees C (trial 2). In Exp. 2, 4 pregnant rumen cannulated cows grazing in January were fitted with Kahne (KB1000) temperature continuous recording boluses for 22 d. Two grazed in a paddock provided cold water (8.2 degrees C) and 2 in a paddock provided warm water (31.1 degrees C). Two in situ trials were conducted placing 6 in situ bags containing 2 g of winter range ruminal extrusa in each of the 4 ruminally cannulated cows and incubating bags for 72 h for measurement of NDF disappearance. In Exp. 3, 6 paddocks (n = 3/water treatment) were grazed by 10 to 13 pregnant crossbred Angus cows from December through February across 3 yr from 2009 to 2012. Water intake per paddock was measured daily and ambient temperature was recorded. Motion-activated cameras were used to determine the time of day water was consumed and the number of cow appearances at water. In Exp. 1, rate and total gas production (P < 0.05) and NDF disappearance (P < 0.001) at 48 h was reduced by each incubation temperature below 39 degrees C. In Exp. 2, ruminal temperature for cows supplied with warm water dropped below 38 degrees C 1.5% of the time whereas ruminal temperature for cows provided cold water dropped below 38 degrees C 9.4% of the time (P < 0.01). Drinking water temperature did not alter in situ OM or NDF disappearance. In Exp. 3, cows with access to warm water consumed 30% (P < 0.05) more water than cows provided cold water. In this study, there were energetic costs to range cows proportional to consumption of water at temperatures less than body temperature. The magnitude of these costs were found to be less than the heat increment because no improvement to BW gain, BCS change, or calf birth weight were found for cows consuming warmed water. C1 [Petersen, M. K.; Muscha, J. M.; Roberts, A. J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Mulliniks, J. T.] Univ Tennessee, Dept Anim Sci, Crossville, TN 38571 USA. RP Petersen, MK (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM Mark.Petersen@ars.usda.gov NR 23 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4297 EP 4306 DI 10.2527/jas2015-0155 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400025 PM 27898880 ER PT J AU Walter, LJ Cole, NA Jennings, JS Hutcheson, JP Meyer, BE Schmitz, AN Reed, DD Lawrence, TE AF Walter, L. J. Cole, N. A. Jennings, J. S. Hutcheson, J. P. Meyer, B. E. Schmitz, A. N. Reed, D. D. Lawrence, T. E. TI The effect of zilpaterol hydrochloride supplementation on energy metabolism and nitrogen and carbon retention of steers fed at maintenance and fasting intake levels SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef; energy metabolism; nutrient digestibility; zilpaterol hydrochloride ID BETA-ADRENERGIC AGONISTS; WET DISTILLERS GRAINS; CORN-BASED DIETS; CARCASS CHARACTERISTICS; METHANE EMISSIONS; FEEDLOT CATTLE; PROTEIN-TURNOVER; FINISHING DIET; MEAT QUALITY; BEEF STEERS AB An indirect calorimetry trial examined energy metabolism, apparent nutrient digestibility, C retention (CR), and N retention (NR) of cattle supplemented with zilpaterol hydrochloride (ZH). Beef steers (n = 20; 463 +/- 14 kg) blocked (n = 5) by weight and source were individually fed and adapted to maintenance energy intake for 21 d before allotment to ZH (90 mg/steerd) or no beta(2)-adrenergic agonist treatment (control [CONT]) for 20 d (455 +/- 14 kg at the start of treatment). Respiration chambers = 4 were used to quantify heat production (HP) during maintenance (d 12 to 16 of the ZH period) and fasting heat production (FHP; d 19 to 20 of ZH period; total 4 d of fast). Steers were harvested after a 6-d ZH withdrawal and carcasses were graded 24 h after harvest. Control cattle lost more BW (P < 0.01; 9 kg for CONT and 2 kg for ZH-treated) during maintenance whereas the BW loss of ZH-treated steers was greater (P < 0.01; 9 kg for ZH-treated and vs. 4 kg, for CONT) during FHP; no differences (P >= 0.76) were detected for G: F, ADG, and end BW. No differences in DMI, apparent nutrient digestibility, O-2 consumption, or CH4 production (P >= 0.12) were detected; however, ZH-treated cattle had greater CO2 production during maintenance (P = 0.04; 23.6 L/kgBW for ZH-treated and 22.4 L/kg BW0.75 for CONT). Digestible energy and ME did not differ (P >= 0.19); however, urinary energy was greater (P = 0.05; 0.091 Mcal for CONT and 0.074 Mcal for ZH-treated) in CONT cattle. Steers treated with ZH tended to have greater HP (P = 0.09; 12.44 Mcal for ZH-treated and 11.69 Mcal for CONT), but the effect was reduced on a BW0.75 basis (P = 0.12; 0.126 Mcal/kg BW0.75 for ZH-treated and 0.120 Mcal/kg BW0.75 for CONT vs. 0.120 Mcal/kg BW0.75). No treatment difference in FHP was observed (P >= 0.32) although CO2 production (L/steer) increased with ZH treatment (P = 0.04; 1,423 L/steer for ZH-treated and 1,338 L/steer for CONT). Control cattle excreted more (P = 0.05) N in urine (39.8 g/d for CONT and 32.4 g/d for ZH-treated); therefore, NR (P = 0.07; 22.14 g/d for ZH-treated and 14.12 g/d for CONT steers) tended to be greater for ZH-fed steers. Steers treated with ZH lost more C via CO2 (P = 0.04; 1,036.9 g/d for ZH-treated and 974.3 g/d for CONT) although total CR did not differ (P >= 0.23). Empty BW, HCW, and harvest yields (g/kg empty BW) were not different (P >= 0.13), whereas ZH increased dressed yield (P = 0.02; 62.12 % for ZH-treated and 60.65% for CONT) and LM area (P = 0.02; 77.81 cm(2) for ZH-treated and vs. 70.90 cm(2) for CONT). Separable carcass lean and actual skeletal muscle protein (SMP) were increased with ZH (P <= 0.04; 201.6 and 41.2 kg, respectively for ZH-treated and 196.0 and 38.4 kg, respectively for CONT). Results from this trial indicate that ZH treatment increased (P = 0.03) SMP and tended (P >= 0.07) to increase NR and modify HP during maintenance by increasing CO2 production. C1 [Walter, L. J.; Schmitz, A. N.; Reed, D. D.; Lawrence, T. E.] West Texas A&M Univ, Beef Carcass Res Ctr, Dept Agr Sci, Canyon, TX 79016 USA. [Cole, N. A.; Meyer, B. E.] USDA ARS, CPRL, Bushland, TX 79012 USA. [Jennings, J. S.] Texas A&M AgriLife Res, Amarillo, TX 79106 USA. [Hutcheson, J. P.] Merck Anim Hlth, Summit, NJ 07901 USA. RP Lawrence, TE (reprint author), West Texas A&M Univ, Beef Carcass Res Ctr, Dept Agr Sci, Canyon, TX 79016 USA. EM tlawrence@wtamu.edu FU Merck Animal Health FX Financial support for this project was provided by Merck Animal Health. J. P. Hutcheson is employed by Merck Animal Health. NR 40 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4401 EP 4414 DI 10.2527/jas2016-0612 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400035 PM 27898856 ER PT J AU Overholt, MF Arkfeld, EK Mohrhauser, DA King, DA Wheeler, TL Dilger, AC Shackelford, SD Boler, DD AF Overholt, M. F. Arkfeld, E. K. Mohrhauser, D. A. King, D. A. Wheeler, T. L. Dilger, A. C. Shackelford, S. D. Boler, D. D. TI Comparison of variability in pork carcass composition and quality between barrows and gilts SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE barrow; composition; gilt; quality; variability ID DISTILLERS DRIED GRAINS; IMMUNOLOGICAL CASTRATION IMPROVEST; FINISHING PIGS; SENSORY CHARACTERISTICS; GROWTH-PERFORMANCE; FAT QUALITY; RACTOPAMINE HYDROCHLORIDE; BELLY CHARACTERISTICS; MEAT QUALITY; SEX AB Pigs (n = 8,042) raised in 8 different barns representing 2 seasons (cold and hot) and 2 production focuses (lean growth and meat quality) were used to characterize variability of carcass composition and quality traits between barrows and gilts. Data were collected on 7,684 pigs at the abattoir. Carcass characteristics, subjective loin quality, and fresh ham face color (muscles) were measured on a targeted 100% of carcasses. Fresh belly characteristics, boneless loin weight, instrumental loin color, and ultimate loin pH measurements were collected from 50% of the carcasses each slaughter day. Adipose tissue iodine value (IV), 30-min loin pH, LM slice shear force, and fresh ham muscle characteristic measurements were recorded on 10% of carcasses each slaughter day. Data were analyzed using the MIXED procedure of SAS as a 1-way ANOVA in a randomized complete block design with 2 levels (barrows and gilts). Barn (block), marketing group, production focus, and season were random variables. A 2-variance model was fit using the REPEATED statement of the MIXED procedure, grouped by sex for analysis of least squares means. Homogeneity of variance was tested on raw data using Levene's test of the GLM procedure. Hot carcass weight of pigs (94.6 kg) in this study was similar to U. S. industry average HCW (93.1 kg). Therefore, these data are representative of typical U. S. pork carcasses. There was no difference (P >= 0.09) in variability of HCW or loin depth between barrow and gilt carcasses. Back fat depth and estimated carcass lean were more variable (P <= 0.0001) and IV was less variable (P = 0.05) in carcasses from barrows than in carcasses from gilts. Fresh belly weight and thickness were more variable (P <= 0.01) for bellies of barrows than bellies of gilts, but there was no difference in variability for belly length, width, or flop distance (P >= 0.06). Fresh loin subjective color was less variable (P < 0.01) and subjective marbling was more variable (P < 0.0001) in loins from barrows than in those from gilts, but there were no differences (P >= 0.08) in variability for any other loin traits or fresh ham traits. Overall, traits associated with carcass fatness, including back fat depth, belly thickness, and marbling, but not IV, were more variable in carcasses from barrows than in carcasses from gilts, whereas minimal differences in variability existed between carcasses of barrows and carcasses of gilts for traits associated with carcass muscling and lean quality. C1 [Overholt, M. F.; Arkfeld, E. K.; Dilger, A. C.; Boler, D. D.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Mohrhauser, D. A.] Smithfield Foods, Denison, IA 51442 USA. [King, D. A.; Wheeler, T. L.; Shackelford, S. D.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Boler, DD (reprint author), Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. EM dboler2@illinois.edu FU National Pork Board FX Funding, wholly or in part, was provided by The National Pork Board. NR 35 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD OCT PY 2016 VL 94 IS 10 BP 4415 EP 4426 DI 10.2527/jas2016-0702 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA ED6GZ UT WOS:000388955400036 PM 27898864 ER PT J AU Fombong, AT Mutunga, JM Teal, PEA Torto, B AF Fombong, Ayuka T. Mutunga, Jacqueline M. Teal, Peter E. A. Torto, Baldwyn TI Behavioral Evidence for Olfactory-Based Location of Honeybee Colonies by the Scarab Oplostomus haroldi SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Coleoptera; Scarabaeidae; Large hive beetle; Esters; Honeybee; Kairomone; Cetoniid ID BEETLE AETHINA-TUMIDA; APIS-MELLIFERA; COLEOPTERA SCARABAEIDAE; LABORATORY CONDITIONS; PACHNODA-INTERRUPTA; RECEPTOR NEURONS; VOLATILES; NITIDULIDAE; DIVERSITY; PHEROMONE AB The Afro-tropical scarab Oplostomus haroldi (Witte) is a pest of honeybees in East Africa with little information available on its chemical ecology. Recently, we identified a female-produced contact sex pheromone, (Z)-9-pentacosene, from the cuticular lipids that attracted males. Here, we investigated the kairomonal basis of host location in O. haroldi. We used coupled gas chromatography/electroantennographic detection (GC/EAD) and GC/mass spectrometry to identify antennally-active compounds from volatiles collected from honeybee colonies. Antennae of both sexes of the beetle consistently detected seven components, which were identified as 3-hydroxy-2-butanone, 2,3-butanediol, butyl acetate, isopentyl acetate, butyl butyrate, hexyl acetate, and methyl benzoate. In olfactometer bioassays, both sexes responded to the full seven-component synthetic blend over solvent controls, but chose honeybee colony odors over the blend. These findings suggest that the seven compounds are components of a kairomone from honeybee colonies used by O. haroldi. C1 [Fombong, Ayuka T.; Mutunga, Jacqueline M.; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol Icipe, POB 30772-00100, Nairobi, Kenya. [Teal, Peter E. A.] USDA ARS, CMAVE, 1600-1700 SW23rd Dr, Gainesville, FL 32608 USA. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol Icipe, POB 30772-00100, Nairobi, Kenya. EM btorto@icipe.org FU German Academic Exchange Service (DAAD) from United States Department of Agriculture - Agricultural Research Service (USDA-ARS) [SCA-586615-7-119F] FX The authors are grateful to Dr. D. Salifu for statistical advice; R. Herisolo for the olfactometer drawing; J. Kilonzo, J. Ngang'a, and A Mwajeve for field assistance with bee odors, frame, and beetle collections from the apiary. The authors are grateful to two anonymous reviewers for comments on an earlier version of the manuscript. The German Academic Exchange Service (DAAD) funded ATF while project funding came from the United States Department of Agriculture - Agricultural Research Service (USDA-ARS Project No. SCA-586615-7-119F). NR 33 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD OCT PY 2016 VL 42 IS 10 BP 1063 EP 1069 DI 10.1007/s10886-016-0748-1 PG 7 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA ED4HB UT WOS:000388807500009 PM 27534749 ER PT J AU Goodrich, BA Waring, KM Kolb, TE AF Goodrich, Betsy A. Waring, Kristen M. Kolb, Thomas E. TI Genetic variation in Pinus strobiformis growth and drought tolerance from southwestern US populations SO TREE PHYSIOLOGY LA English DT Article DE adaptive traits; carbon isotope ratio; five-needle pine; southwestern white pine ID CLIMATE-CHANGE; BLISTER RUST; DOUGLAS-FIR; TREE POPULATIONS; NORTH-AMERICA; GAS-EXCHANGE; FOREST; RESPONSES; PONDEROSA; SEEDLINGS AB The persistence of some tree species is threatened by combinations of novel abiotic and biotic stressors. To examine the hypothesis that Pinus strobiformis Engelm., a tree threatened by an invasive forest pathogen and a changing climate, exhibits intraspecific genetic variation in adaptive traits, we conducted a common garden study of seedlings at one location with two watering regimes using 24 populations. Four key findings emerged: (i) growth and physiological traits were low to moderately differentiated among populations but differentiation was high for some traits in water-stressed populations; (ii) seedlings from warmer climates grew larger, had higher stomatal density and were more water-use efficient (as measured by the carbon isotope ratio) than populations from colder climates; (iii) seedlings from the northern edge of the species' distribution had lower water-use efficiency, higher stomatal conductance, slower growth and longer survival in a lethal drought experiment compared with seedlings from more southern populations; and (iv) based on non-metric multidimensional scaling analyses, populations clustered into southern and northern groups, which did not correspond to current seed transfer zones. Our discovery of a clinal geographic pattern of genetic variation in adaptive traits of P. strobiformis seedlings will be useful in developing strategies to maintain the species during ongoing climate change and in the face of an invasive pathogen. C1 [Goodrich, Betsy A.; Waring, Kristen M.; Kolb, Thomas E.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Goodrich, Betsy A.] US Forest Serv, Forest Hlth Protect, USDA, Wenatchee Forestry Sci Lab, 1133 N Western Ave, Wenatchee, WA 98801 USA. RP Goodrich, BA (reprint author), No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA.; Goodrich, BA (reprint author), US Forest Serv, Forest Hlth Protect, USDA, Wenatchee Forestry Sci Lab, 1133 N Western Ave, Wenatchee, WA 98801 USA. EM agoodrich@fs.fed.us FU McIntire-Stennis; State of Arizona, a Genes to Environment Fellowship through NAU Department of Biology; USDA USFS Forest Health Protection FX Funding for this project was provided by McIntire-Stennis appropriations to NAU and the State of Arizona, a Genes to Environment Fellowship through NAU Department of Biology, and the USDA USFS Forest Health Protection. NR 62 TC 0 Z9 0 U1 8 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD OCT PY 2016 VL 36 IS 10 BP 1219 EP 1235 DI 10.1093/treephys/tpw052 PG 17 WC Forestry SC Forestry GA ED6CK UT WOS:000388942100004 PM 27344065 ER PT J AU Royo, AA Kramer, DW Miller, KV Nibbelink, NP Stout, SL AF Royo, Alejandro A. Kramer, David W. Miller, Karl V. Nibbelink, Nathan P. Stout, Susan L. TI The canary in the coal mine: Sprouts as a rapid indicator of browse impact in managed forests SO ECOLOGICAL INDICATORS LA English DT Article DE Odocoileus virginianus; Deer; Herbivory; Stump sprout; Disturbance ID WHITE-TAILED DEER; EASTERN DECIDUOUS FOREST; PELLET-GROUP COUNTS; ECOLOGICAL IMPACTS; HERBIVORE DENSITY; SHADE TOLERANCE; TREE GROWTH; VEGETATION; REGENERATION; DISTURBANCE AB Forest managers are frequently confronted with sustaining vegetation diversity and structure in landscapes experiencing high ungulate browsing pressure. Often, managers monitor browse damage and risk to plant communities using vegetation as indicators (i.e., phytoindicators). Although useful, the efficacy of traditional phytoindicators is sometimes hampered by limited distribution and abundance, variable browse susceptibility, and lagged responses. In contrast, sprouts possess traits which make them readily available and attractive to browsers, yet fairly resilient to tissue loss. Here, we experimentally evaluate whether hardwood tree stump sprouts are effective and sensitive phytoindicators of deer browse pressure. We measured sprout abundance and height in fenced and unfenced plots at 17 shelterwood harvested sites scattered across a 6500 km(2) region where deer densities varied by nearly an order of magnitude. We found browsing did not alter the proportion of stumps sprouting and sprout density; however, browse pressure varied among the four most abundant species. Acer rubrum and Acer saccharum were heavily browsed, although browse pressure on A. saccharum decreased in areas with greater canopy openness. Fagus grandifolia and Prunus serotina were less preferred. Differences in palatability altered size hierarchies. Averaged across all species, browsing reduced sprout height by 39%, relative to protected sprouts. Under ambient browsing, P. serotina was 60-100% taller than other species and significantly taller than A. saccharum and F. grandifolia. However, within fences A. saccharum and A. rubrum doubled in size, relative to browsed individuals, and were as tall as P. serotina. Deer impact on sprout height within unfenced forest stands was negatively correlated with estimated deer densities (R-2=0.46). Thus, we suggest sprout surveys can provide a measure of impact across much larger areas. Our results demonstrate that sprouts, particularly those of Acer species, offer an abundant, easily measured, and reliable indicator of browse pressure. Moreover, browse impacts on sprouts emerged before impacts were detected on seedling abundance, height, or biomass. We argue sprouts can warn of imminent browse risk to seedlings (and perhaps non-woody vegetation) and thereby allow managers to take actions to mitigate or avert losses to the regenerating seedling cohort. Published by Elsevier Ltd. C1 [Royo, Alejandro A.; Stout, Susan L.] USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. [Kramer, David W.; Miller, Karl V.; Nibbelink, Nathan P.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Royo, AA (reprint author), USDA Forest Serv, Northern Res Stn, POB 267, Irvine, PA 16329 USA. EM aroyo@fs.fed.us FU USDA Forest Service Northern Research Station; University of Georgia Warnell School of Forestry and Natural Resources; USDA-AFRI Award [12-IA-11242302-093] FX This work was supported by the USDA Forest Service Northern Research Station, the University of Georgia Warnell School of Forestry and Natural Resources, and USDA-AFRI Award #12-IA-11242302-093. We are indebted to the Allegheny National Forest, the Pennsylvania Bureau of Forestry, Bradford Water Authority, Forest Investment Associates, Generations Forestry, Hancock Forest Management, Landvest, and Kane Hardwoods for field sites. We thank Charles Vandever for assisting with data collection and Todd Ristau, Scott Stoleson, Patricia Raymond, Tara Keyser, and two anonymous reviewers for valuable comments on prior versions of this manuscript. NR 77 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD OCT PY 2016 VL 69 BP 269 EP 275 DI 10.1016/j.ecolind.2016.04.030 PG 7 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YT UT WOS:000388785100027 ER PT J AU Williamson, JC Bestelmeyer, BT McClaran, MP Robinett, D Briske, DD Wu, XB Fernandez-Gimenez, ME AF Williamson, Jeb C. Bestelmeyer, Brandon T. McClaran, Mitchel P. Robinett, Dan Briske, David D. Wu, X. Ben Fernandez-Gimenez, Maria E. TI Can ecological land classification increase the utility of vegetation monitoring data? SO ECOLOGICAL INDICATORS LA English DT Article DE Biological invasion; Land classification; Monitoring; Soil texture; Time series; Vegetation indicator ID TRANSITION MODELS; DESERT GRASSLAND; SOIL TEXTURE; DYNAMICS; ENCROACHMENT; MANAGEMENT; RANGELANDS; SITES; AUSTRALIA; ECOSYSTEM AB Vegetation dynamics in rangelands and other ecosystems are known to be mediated by topoedaphic properties. Vegetation monitoring programs, however, often do not consider the impact of soils and other sources of landscape heterogeneity on the temporal patterns observed. Ecological sites (ES) comprise a land classification system based on soil, topographic, and climate variations that can be readily applied by land managers to classify topoedaphic properties at monitoring locations. We used a long-term (>40y) vegetation record from southeastern Arizona, USA to test the utility of an ES classification for refining interpretations of monitoring data in an area of relatively subtle soil differences. We focused on two phenomena important to rangeland management in the southeastern Arizona region: expansion of the native tree velvet mesquite (Prosopis velutina Woot.) and spread of the introduced perennial grass Lehmann lovegrass (Eragrostis lehmanniana Nees). Specifically, we sought to determine if a quantitative, ES-specific analysis of the long-term record would (1) improve detection of changes in plant species having heightened ecological or management importance and (2) further clarify topoedaphic effects on vegetation trajectories. We found that ES class membership was a significant factor explaining spatiotemporal variation in velvet mesquite canopy cover, Lehmann lovegrass basal cover, and Lehmann lovegrass density measurements. In addition, we observed that the potential magnitude of velvet mesquite and Lehmann lovegrass increases varied substantially among ES classes. Our study brings attention to a practical land management tool that might be called upon to increase the effectiveness of vegetation-based indicators of ecosystem change. Published by Elsevier Ltd. C1 [Williamson, Jeb C.; Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. [McClaran, Mitchel P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Robinett, Dan] Robinett Rangeland Resources LLC, Elgin, AZ USA. [Briske, David D.; Wu, X. Ben] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX USA. [Fernandez-Gimenez, Maria E.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. RP Williamson, JC (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM jcwill@nmsu.edu FU USDA CSREES [2007-38415-18637, 2008-51130-19567] FX This research was supported in part by USDA CSREES grants 2007-38415-18637 and 2008-51130-19567. NR 49 TC 1 Z9 1 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD OCT PY 2016 VL 69 BP 657 EP 666 DI 10.1016/j.ecolind.2016.05.030 PG 10 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YT UT WOS:000388785100064 ER PT J AU Clifford, JC Buchanan, A Vining, O Kidarsa, TA Chang, JH McPhail, KL Loper, JE AF Clifford, Jennifer C. Buchanan, Alex Vining, Oliver Kidarsa, Teresa A. Chang, Jeff H. McPhail, Kerry L. Loper, Joyce E. TI Phloroglucinol functions as an intracellular and intercellular chemical messenger influencing gene expression in Pseudomonas protegens SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ANTIFUNGAL METABOLITE PRODUCTION; SIGMA-FACTOR SIGMA(S); PYTHIUM DAMPING-OFF; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; ESCHERICHIA-COLI; PYOLUTEORIN PRODUCTION; ANTIBIOTIC PRODUCTION; CELL COMMUNICATION; COTTON SEEDLINGS AB Bacteria can be both highly communicative and highly competitive in natural habitats and antibiotics are thought to play a role in both of these processes. The soil bacterium Pseudomonas protegens Pf-5 produces a spectrum of antibiotics, two of which, pyoluteorin and 2,4-diacetylphloroglucinol (DAPG), function in intracellular and intercellular communication, both as autoinducers of their own production. Here, we demonstrate that phloroglucinol, an intermediate in DAPG biosynthesis, can serve as an intercellular signal influencing the expression of pyoluteorin biosynthesis genes, the production of pyoluteorin, and inhibition of Pythium ultimum, a phytopathogenic oomycete sensitive to pyoluteorin. Through analysis of RNAseq data sets, we show that phloroglucinol had broad effects on the transcriptome of Pf-5, significantly altering the transcription of more than two hundred genes. The effects of nanomolar versus micromolar concentrations of phloroglucinol differed both quantitatively and qualitatively, influencing the expression of distinct sets of genes or having opposite effects on transcript abundance of certain genes. Therefore, our results support the concept of hormesis, a phenomenon associated with signalling molecules that elicit distinct responses at different concentrations. Phloroglucinol is the first example of an intermediate of antibiotic biosynthesis that functions as a chemical messenger influencing gene expression in P. protegens. C1 [Clifford, Jennifer C.; Kidarsa, Teresa A.; Loper, Joyce E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Buchanan, Alex; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Buchanan, Alex; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Vining, Oliver; McPhail, Kerry L.] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. RP Loper, JE (reprint author), ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA.; Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Loper, JE (reprint author), Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. EM joyce.loper@ars.usda.gov OI Loper, Joyce/0000-0003-3501-5969 FU National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67019-30192] FX We are grateful to Jeff Kimbrel, Allison Creason, Brenda Shaffer, Ashley Bixenstein and Marcella Henkels for their assistance; Jerry Weiland for advice on the assays evaluating inhibition of Pythium ultimum; and Mark Desenko, Matthew Peterson and Chris Sullivan of the Center for Genomics Research and Biocomputing at Oregon State University for sequencing expertise and services. This project was supported by National Research Initiative Competitive Grant 2011-67019-30192 from the USDA National Institute of Food and Agriculture. NR 73 TC 2 Z9 2 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3296 EP 3308 DI 10.1111/1462-2920.13043 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200009 PM 26337778 ER PT J AU Song, CX Kidarsa, TA van de Mortel, JE Loper, JE Raaijmakers, JM AF Song, Chunxu Kidarsa, Teresa A. van de Mortel, Judith E. Loper, Joyce E. Raaijmakers, Jos M. TI Living on the edge: emergence of spontaneous gac mutations in Pseudomonas protegens during swarming motility SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FLUORESCENS PF-5; CELL-DIFFERENTIATION; PROTEUS-MIRABILIS; PATHOGENIC BACTERIA; MYXOCOCCUS-XANTHUS; PHASE VARIATION; AERUGINOSA; MUTANTS; COLONIZATION; RECOGNITION AB Swarming motility is a flagella-driven multicellular behaviour that allows bacteria to colonize new niches and escape competition. Here, we investigated the evolution of specific mutations in the GacS/GacA two-component regulatory system in swarming colonies of Pseudomonas protegens Pf-5. Experimental evolution assays showed that repeated rounds of swarming by wildtype Pf-5 drives the accumulation of gacS/gacA spontaneous mutants on the swarming edge. These mutants cannot swarm on their own because they lack production of the biosurfactant orfamide A, but they do co-swarm with orfamide-producing wildtype Pf-5. These co-swarming assays further demonstrated that DgacA mutant cells indeed predominate on the edge and that initial DgacA: wildtype Pf-5 ratios of at least 2: 1 lead to a collapse of the swarming colony. Subsequent whole-genome transcriptome analyses revealed that genes associated with motility, resource acquisition, chemotaxis and efflux were significantly upregulated in DgacA mutant on swarming medium. Moreover, transmission electron microscopy showed that DgacA mutant cells were longer and more flagellated than wildtype cells, which may explain their predominance on the swarming edge. We postulate that adaptive evolution through point mutations is a common feature of range-expanding microbial populations and that the putative fitness benefits of these mutations during dispersal of bacteria into new territories are frequency-dependent. C1 [Song, Chunxu; Raaijmakers, Jos M.] Netherlands Inst Ecol, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands. [Song, Chunxu; van de Mortel, Judith E.] Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. [Kidarsa, Teresa A.; Loper, Joyce E.] ARS, USDA, Corvallis, OR 97330 USA. [Raaijmakers, Jos M.] Leiden Univ, Microbial Biotechnol Dept, Inst Biol IBL, Leiden, Netherlands. [van de Mortel, Judith E.] HAS Univ Appl Sci, NL-5911 KJ Venlo, Netherlands. RP Raaijmakers, JM (reprint author), Netherlands Inst Ecol, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands.; Raaijmakers, JM (reprint author), Leiden Univ, Microbial Biotechnol Dept, Inst Biol IBL, Leiden, Netherlands. EM j.raaijmakers@nioo.knaw.nl RI Raaijmakers, Jos/D-1574-2014; OI Raaijmakers, Jos/0000-0003-1608-6614; KNAW, NIOO-KNAW/0000-0002-3835-159X; Loper, Joyce/0000-0003-3501-5969 FU Graduate School of Experimental Plant Sciences (EPS); Organisation for Economic Cooperation and Development (OECD) FX We are very grateful to the Graduate School of Experimental Plant Sciences (EPS) and the Organisation for Economic Cooperation and Development (OECD) for partially financing this project. We thank the Dutch Genomics Service & Support Provider for conducting the microarray analysis. We thank Marcel Giesbers (Wageningen Electron Microscopy Centre, The Netherlands) for conducting the transmission electron microscopy, Ester Dekkers (Phytopathology Department, Wageningen University) for genetically characterizing the mutations in the spontaneous mutants, Stephen Heeb and Dieter Haas for providing pME6000, and Dr. Irene de Bruijn, Dr. Daniel Roozen and Dr. Arjan de Visser for critically reading the manuscript and excellent suggestions. This publication is no. 6041 of The Netherlands Institute of Ecology (NIOO-KNAW). NR 46 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3453 EP 3465 DI 10.1111/1462-2920.13288 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200019 PM 26945503 ER PT J AU Loper, JE Henkels, MD Rangel, LI Olcott, MH Walker, FL Bond, KL Kidarsa, TA Hesse, CN Sneh, B Stockwell, VO Taylor, BJ AF Loper, Joyce E. Henkels, Marcella D. Rangel, Lorena I. Olcott, Marika H. Walker, Francesca L. Bond, Kise L. Kidarsa, Teresa A. Hesse, Cedar N. Sneh, Baruch Stockwell, Virginia O. Taylor, Barbara J. TI Rhizoxin analogs, orfamide A and chitinase production contribute to the toxicity of Pseudomonas protegens strain Pf-5 to Drosophila melanogaster SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ANTIFUNGAL METABOLITE PRODUCTION; PLANT-ASSOCIATED PSEUDOMONADS; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; GENE-CLUSTER; PERITROPHIC MATRIX; COTTON SEEDLINGS; INSECT TOXIN; DAMPING-OFF; IDENTIFICATION AB Pseudomonas protegens strain Pf-5 is a soil bacterium that was first described for its capacity to suppress plant diseases and has since been shown to be lethal to certain insects. Among these is the common fruit fly Drosophila melanogaster, a well-established model organism for studies evaluating the molecular and cellular basis of the immune response to bacterial challenge. Pf-5 produces the insect toxin FitD, but a Delta fitD mutant of Pf-5 retained full toxicity against D. melanogaster in a noninvasive feeding assay, indicating that FitD is not a major determinant of Pf-5's oral toxicity against this insect. Pf-5 also produces a broad spectrum of exoenzymes and natural products with antibiotic activity, whereas a mutant with a deletion in the global regulatory gene gacA produces none of these exoproducts and also lacks toxicity to D. melanogaster. In this study, we made use of a panel of Pf-5 mutants having single or multiple mutations in the biosynthetic gene clusters for seven natural products and two exoenzymes that are produced by the bacterium under the control of gacA. Our results demonstrate that the production of rhizoxin analogs, orfamide A, and chitinase are required for full oral toxicity of Pf-5 against D. melanogaster, with rhizoxins being the primary determinant. C1 [Loper, Joyce E.; Henkels, Marcella D.; Kidarsa, Teresa A.; Hesse, Cedar N.] ARS, USDA, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Loper, Joyce E.; Henkels, Marcella D.; Rangel, Lorena I.; Kidarsa, Teresa A.; Stockwell, Virginia O.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Olcott, Marika H.; Walker, Francesca L.; Bond, Kise L.; Taylor, Barbara J.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Sneh, Baruch] Tel Aviv Univ, Dept Mol Biol & Ecol Plants, George S Wise Fac Life Sci, Tel Aviv, Israel. RP Loper, JE (reprint author), ARS, USDA, 3420 NW Orchard Ave, Corvallis, OR 97330 USA.; Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM loperj@science.oregonstate.edu OI Loper, Joyce/0000-0003-3501-5969 FU Agriculture and Food Research Initiative Competitive Grants from the United States Department of Agriculture National Institute of Food and Agriculture [2006-35319-17427, 2011-67019-30192] FX We gratefully acknowledge the assistance of Brenda Shaffer, Ashley Bixenstein and Max Kohen in carrying out the experiments described herein. We also thank Donald Kobayashi of Rutgers, the State University of New Jersey, for providing a chiC mutant of Pf-5 for this study. This work was supported by Agriculture and Food Research Initiative Competitive Grants 2006-35319-17427 and 2011-67019-30192 from the United States Department of Agriculture National Institute of Food and Agriculture. NR 69 TC 3 Z9 3 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD OCT PY 2016 VL 18 IS 10 SI SI BP 3509 EP 3521 DI 10.1111/1462-2920.13369 PG 13 WC Microbiology SC Microbiology GA ED1NW UT WOS:000388613200023 PM 27130686 ER PT J AU Barnett, K Parks, SA Miller, C Naughton, HT AF Barnett, Kevin Parks, Sean A. Miller, Carol Naughton, Helen T. TI Beyond Fuel Treatment Effectiveness: Characterizing Interactions between Fire and Treatments in the US SO FORESTS LA English DT Article DE encounter rate; treatment maintenance; treatment longevity; MTBS; LANDFIRE; wildland fire ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; SOUTHERN SIERRA-NEVADA; MIXED-CONIFER FOREST; BURNED AREA; WILDFIRE; CALIFORNIA; LANDSCAPE; SEVERITY; PROBABILITY AB In the United States, fuel reduction treatments are a standard land management tool to restore the structure and composition of forests that have been degraded by past management. Although treatments can have multiple purposes, their principal objective is to create landscape conditions where wildland fire can be safely managed to help achieve long-term land management goals. One critique is that fuel treatment benefits are unlikely to transpire due to the low probability that treated areas will be burned by a subsequent fire within a treatment's lifespan, but little quantitative information exists to corroborate this argument. We summarized the frequency, extent, and geographic variation of fire and fuel treatment interactions on federal lands within the conterminous United States (CONUS). We also assessed how the encounters between fuel treatments and fires varied with treatment size, treatment age, and number of times treated. Overall, 6.8% of treatment units evaluated were encountered by a subsequent fire during the study period, though this rate varied among ecoregions across the CONUS. Larger treatment units were more likely to be encountered by a fire, and treatment units were most frequently burned within one year of the most recent treatment, the latter of which is likely because of ongoing maintenance of existing treatments. Our results highlight the need to identify and prioritize additional opportunities to reduce fuel loading and fire risk on the millions of hectares of federal lands in the CONUS that are in need of restoration. C1 [Barnett, Kevin; Naughton, Helen T.] Univ Montana, Dept Econ, Missoula, MT 59701 USA. [Parks, Sean A.; Miller, Carol] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. RP Barnett, K (reprint author), Univ Montana, Dept Econ, Missoula, MT 59701 USA. EM kevin.barnett@umontana.edu; sean_parks@fs.fed.us; cmiller04@fs.fed.us; helen.naughton@mso.umt.edu FU Joint Fire Science Program [14-5-01-25] FX We thank two anonymous reviewers for thoughtful comments that significantly improved the manuscript. Funding for this research was provided by the Joint Fire Science Program under Project 14-5-01-25. NR 55 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 237 DI 10.3390/f7100237 PG 12 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800028 ER PT J AU Dang, M Zhang, T Hu, YH Zhou, HJ Woeste, KE Zhao, P AF Dang, Meng Zhang, Tian Hu, Yiheng Zhou, Huijuan Woeste, Keith E. Zhao, Peng TI De Novo Assembly and Characterization of Bud, Leaf and Flowers Transcriptome from Juglans Regia L. for the Identification and Characterization of New EST-SSRs SO FORESTS LA English DT Article DE microsatellites; transcriptome; next-generation sequencing; genetic diversity; English walnut ID RNA-SEQ DATA; DATA PROVIDES INSIGHT; MICROSATELLITE MARKERS; GENETIC DIVERSITY; DROUGHT RESPONSE; WALNUT CULTIVARS; ANNOTATION; DISCOVERY; SOFTWARE; PROGRAM AB Persian walnut (Juglans regia L.), valued for both its nut and wood, is an ecologically important temperate tree species native to the mountainous regions of central Asia. Despite its importance, there are still few transcriptomic resources in public databases for J. regia, limiting gene discovery and breeding. Here, more than 49.9 million sequencing reads were generated using Illumina sequencing technology in the characterization of the transcriptome of four J. regia organs (bud, leaf, female flowers, and male flowers). De novo assembly yielded 117,229 unigenes with an N50 of 1955 bp. Based on sequence similarity searches against known proteins, a total of 20,413 (17.41%) genes were identified and annotated. A set of 27,584 unigenes with SSR (simple sequence repeats) motifs were identified as potential molecular markers, and a sample of 77 of these EST-SSRs (express sequence tags) were further evaluated to validate their amplification and assess their polymorphism. Next, we developed 39 polymorphic microsatellite markers to screen 88 Persian walnut individuals collected from 11 populations. These markers and transcriptomic resources will be useful for future studies of population genetic structure, evolutionary ecology, and breeding of Persian walnut and other Juglans species. C1 [Dang, Meng; Zhang, Tian; Hu, Yiheng; Zhou, Huijuan; Zhao, Peng] Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, Xian 710069, Shanxi, Peoples R China. [Woeste, Keith E.] Purdue Univ, USDA, Forest Serv, HTIRC,Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. RP Zhao, P (reprint author), Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Minist Educ, Coll Life Sci, Xian 710069, Shanxi, Peoples R China.; Woeste, KE (reprint author), Purdue Univ, USDA, Forest Serv, HTIRC,Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. EM 15339260798@163.com; 13239166791@163.com; berlin@stumail.nwu.edu.cn; nandehuturen@163.com; woeste@purdue.edu; pengzhao@nwu.edu.cn FU National Natural Science Foundation of China [31200500, 41471038, J1210063]; Changjiang Scholars and Innovative Research Team in University [IRT1174]; Program for Excellent Young Academic Backbones - Northwest University [338050070]; Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates [2015159, 2016171] FX The authors wish to thank Jia Yang, Li Feng, Hailong Xia, Qiang Zhang, and Tao Zhou for sample collection. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. This work was supported by the National Natural Science Foundation of China (Grant No. 31200500; Grant No. 41471038; Grant No. J1210063), Changjiang Scholars and Innovative Research Team in University (No. IRT1174), the Program for Excellent Young Academic Backbones funding by Northwest University (Grant No. 338050070), and the Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates (Grant No. 2015159 and Grand No. 2016171). NR 61 TC 0 Z9 0 U1 13 U2 13 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 247 DI 10.3390/f7100247 PG 17 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800037 ER PT J AU Marschall, JM Stambaugh, MC Jones, BC Guyette, RP Brose, PH Dey, DC AF Marschall, Joseph M. Stambaugh, Michael C. Jones, Benjamin C. Guyette, Richard P. Brose, Patrick H. Dey, Daniel C. TI Fire Regimes of Remnant Pitch Pine Communities in the Ridge and Valley Region of Central Pennsylvania, USA SO FORESTS LA English DT Article DE Pennsylvania; dendrochronology; fire scars; fire severity; humans ID EASTERN UNITED-STATES; NORTH-CENTRAL PENNSYLVANIA; LAND-USE; AMERICAN FORESTS; PRESCRIBED FIRE; OAK FORESTS; HISTORY; CLIMATE; MOUNTAINS; DYNAMICS AB Many fire-adapted ecosystems in the northeastern U.S. are converting to fire-intolerant vegetation communities due to fire suppression in the 20th century. Prescribed fire and other vegetation management activities that increase resilience and resistance to global changes are increasingly being implemented, particularly on public lands. For many fire-dependent communities, there is little quantitative data describing historical fire regime attributes such as frequency, severity, and seasonality, or how these varied through time. Where available, fire-scarred live and remnant trees, including stumps and snags, offer valuable insights into historical fire regimes through tree-ring and fire-scar analyses. In this study, we dated fire scars from 66 trees at two sites in the Ridge and Valley Province of the Appalachian Mountains in central Pennsylvania, and described fire frequency, severity, and seasonality from the mid-17th century to 2013. Fires were historically frequent, of low to moderate severity, occurred mostly during the dormant season, and were influenced by aspect and topography. The current extended fire-free interval is unprecedented in the previous 250-300 years at both sites. C1 [Marschall, Joseph M.; Stambaugh, Michael C.; Guyette, Richard P.] Univ Missouri, Sch Nat Resources, Missouri Tree Ring Lab, 203 ABNR Bldg, Columbia, MO 65211 USA. [Jones, Benjamin C.] Penn Game Commiss, Habitat Planning & Dev Div, Harrisburg, PA 17110 USA. [Brose, Patrick H.; Dey, Daniel C.] US Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. RP Marschall, JM (reprint author), Univ Missouri, Sch Nat Resources, Missouri Tree Ring Lab, 203 ABNR Bldg, Columbia, MO 65211 USA. EM marschallj@missouri.edu; stambaughm@missouri.edu; benjjones@pa.gov; guyetter@missouri.edu; pbrose@fs.fed.us; ddey@fs.fed.us FU U.S. Forest Service, Northern Research Station; Pennsylvania Game Commission FX Identification of study sites was supported by the U.S. Forest Service, Northern Research Station. This research was funded by the Pennsylvania Game Commission. We thank David Matthew Bourscheidt for data collection assistance in the field, Erin Abadir for help identifying cultural and land-use periods, and Chris Bobryk for cartography assistance. We are grateful for contributions from two anonymous reviewers, which improved the manuscript. NR 80 TC 0 Z9 0 U1 6 U2 6 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD OCT PY 2016 VL 7 IS 10 AR 224 DI 10.3390/f7100224 PG 16 WC Forestry SC Forestry GA ED2JQ UT WOS:000388670800015 ER PT J AU Corson-Rikert, HA Wondzell, SM Haggerty, R Santelmann, MV AF Corson-Rikert, Hayley A. Wondzell, Steven M. Haggerty, Roy Santelmann, Mary V. TI Carbon dynamics in the hyporheic zone of a headwater mountain stream in the Cascade Mountains, Oregon SO WATER RESOURCES RESEARCH LA English DT Article DE hyporheic; dissolved inorganic carbon; dissolved organic carbon ID DISSOLVED ORGANIC-CARBON; VERTICAL HYDROLOGIC EXCHANGE; INORGANIC CARBON; WATER CHEMISTRY; METABOLISM; FLUXES; FOREST; FLOW; SOIL; GROUNDWATER AB We investigated carbon dynamics in the hyporheic zone of a steep, forested, headwater catchment western Oregon, USA. Water samples were collected monthly from the stream and a well network during base flow periods. We examined the potential for mixing of different source waters to explain concentrations of DOC and DIC. We did not find convincing evidence that either inputs of deep groundwater or lateral inputs of shallow soil water influenced carbon dynamics. Rather, carbon dynamics appeared to be controlled by local processes in the hyporheic zone and overlying riparian soils. DOC concentrations were low in stream water (0.04-0.09 mM), and decreased with nominal travel time through the hyporheic zone (0.02-0.04 mM lost over 100 h). Conversely, stream water DIC concentrations were much greater than DOC (0.35-0.7 mM) and increased with nominal travel time through the hyporheic zone (0.2-0.4 mM gained over 100 h). DOC in stream water could only account for 10% of the observed increase in DIC. In situ metabolic processing of buried particulate organic matter as well as advection of CO2 from the vadose zone likely accounted for the remaining 90% of the increase in DIC. Overall, the hyporheic zone was a source of DIC to the stream. We suggest that, in mountain stream networks, hyporheic exchange facilitates the transformation of particulate organic carbon buried in floodplains and transports the DIC that is produced back to the stream where it can be evaded to the atmosphere. C1 [Corson-Rikert, Hayley A.; Haggerty, Roy; Santelmann, Mary V.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR USA. [Wondzell, Steven M.] US Forest Serv, Pacific Northwest Res Stn, Corvallis, OR USA. RP Corson-Rikert, HA (reprint author), Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR USA. EM hacorrik@gmail.com RI Haggerty, Roy/A-5863-2009 FU U.S. Forest Service Pacific Northwest Research Station; Water Resources Graduate Program; Oregon State University Provost's Distinguished Fellowship; National Science Foundation [EAR 1417603]; National Science Foundation's Long-Term Ecological Research Program [DEB 0823380]; Oregon State University; [10-JV-11261991-055-PNW] FX Following publication, all data will be made available through the H.J. Andrews Experimental Forest data catalog, which can be accessed via the following url: http://andrewsforest.oregonstate.edu/lter/data.cfm?frameURL=8. We also cited unpublished data collected by Serchan and Pennington. Satish Serchan can be reached at satish.serchan@gmail.com, and Robert Pennington can be reached at robertopennington@gmail.com. Direct support for this work was provided by agreement 10-JV-11261991-055-PNW. Additional funding and support for this research was provided by the U.S. Forest Service Pacific Northwest Research Station, the Water Resources Graduate Program, the Oregon State University Provost's Distinguished Fellowship, and the National Science Foundation (EAR 1417603). Data and research facilities were provided by the H.J. Andrews Experimental Forest research program, which is funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 0823380), the U.S. Forest Service Pacific Northwest Research Station, and Oregon State University. We thank Nick Dosch for help with fieldwork, Lisa Ganio for help with data analysis, Noah Schmadel and Adam Ward who provided analyses of watershed drainage areas and UAA, both the H.J. Andrews staff and the LTER research network for data and field support, and the IWW Collaboratory and Kathy Motter for laboratory equipment and training, as well as technical support. NR 66 TC 0 Z9 0 U1 14 U2 14 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD OCT PY 2016 VL 52 IS 10 BP 7556 EP 7576 DI 10.1002/2016WR019303 PG 21 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA EC9VW UT WOS:000388493400002 ER PT J AU Miller, MP Boyer, EW McKnight, DM Brown, MG Gabor, RS Hunsaker, CT Iavorivska, L Inamdar, S Johnson, DW Kaplan, LA Lin, H McDowell, WH Perdrial, JN AF Miller, Matthew P. Boyer, Elizabeth W. McKnight, Diane M. Brown, Michael G. Gabor, Rachel S. Hunsaker, Carolyn T. Iavorivska, Lidiia Inamdar, Shreeram Johnson, Dale W. Kaplan, Louis A. Lin, Henry McDowell, William H. Perdrial, Julia N. TI Variation of organic matter quantity and quality in streams at Critical Zone Observatory watersheds SO WATER RESOURCES RESEARCH LA English DT Article DE DOM; critical zone; atmospheric deposition; fluorescence ID NORTHEASTERN UNITED-STATES; ECOSYSTEM METABOLISM; CHEMICAL-COMPOSITION; ACID DEPOSITION; CARBON-CYCLE; FULVIC-ACIDS; SOILS; CATCHMENT; LAKES; BIODEGRADABILITY AB The quantity and chemical composition of dissolved organic matter (DOM) in surface waters influence ecosystem processes and anthropogenic use of freshwater. However, despite the importance of understanding spatial and temporal patterns in DOM, measures of DOM quality are not routinely included as part of large-scale ecosystem monitoring programs and variations in analytical procedures can introduce artifacts. In this study, we used consistent sampling and analytical methods to meet the objective of defining variability in DOM quantity and quality and other measures of water quality in streamflow issuing from small forested watersheds located within five Critical Zone Observatory sites representing contrasting environmental conditions. Results show distinct separations among sites as a function of water quality constituents. Relationships among rates of atmospheric deposition, water quality conditions, and stream DOM quantity and quality are consistent with the notion that areas with relatively high rates of atmospheric nitrogen and sulfur deposition and high concentrations of divalent cations result in selective transport of DOM derived from microbial sources, including in-stream microbial phototrophs. We suggest that the critical zone as a whole strongly influences the origin, composition, and fate of DOM in streams. This study highlights the value of consistent DOM characterization methods included as part of long-term monitoring programs for improving our understanding of interactions among ecosystem processes as controls on DOM biogeochemistry. C1 [Miller, Matthew P.; Boyer, Elizabeth W.; Brown, Michael G.; Iavorivska, Lidiia; Lin, Henry] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Miller, Matthew P.] US Geol Survey, Utah Water Sci Ctr, Salt Lake City, UT USA. [McKnight, Diane M.; Gabor, Rachel S.] Univ Colorado, Dept Civil Environm & Architectural Engn, Boulder, CO 80309 USA. [Hunsaker, Carolyn T.] US Forest Serv, USDA, Pacific Southwest Res Stn, Fresno, CA USA. [Inamdar, Shreeram] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19717 USA. [Johnson, Dale W.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Kaplan, Louis A.] Stroud Water Res Ctr, Avondale, PA USA. [McDowell, William H.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Perdrial, Julia N.] Univ Vermont, Dept Geol, Burlington, VT USA. RP Miller, MP (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA.; Miller, MP (reprint author), US Geol Survey, Utah Water Sci Ctr, Salt Lake City, UT USA. EM mamiller@usgs.gov RI McDowell, William/E-9767-2010; Lin, Henry/E-8234-2011; OI McDowell, William/0000-0002-8739-9047; Iavorivska, Lidiia/0000-0002-6224-6271 FU National Science Foundation [Southern Sierra: EAR-0725097, Boulder: EAR-0724960, Shale Hills: EAR-0725019, EAR12-39285, EAR-1331726, Christina River EAR-0724971, EAR-0809205, Luquillo: EAR-0722476, EAR-1331841] FX We thank Eric Parrish, Matt Meadows, Shatrughan Singh, John Bithorn, Jeff Grimm, and Tim White for technical assistance. Helpful comments on an earlier draft of this manuscript were provided by Douglas Burns and three anonymous reviewers. This work was supported in part by the National Science Foundation (Southern Sierra: EAR-0725097; Boulder: EAR-0724960; Shale Hills: EAR-0725019, EAR12-39285, EAR-1331726; Christina River EAR-0724971, EAR-0809205; Luquillo: EAR-0722476, EAR-1331841). All data from this paper are included here as Supporting Information. NR 83 TC 0 Z9 0 U1 12 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD OCT PY 2016 VL 52 IS 10 BP 8202 EP 8216 DI 10.1002/2016WR018970 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA EC9VW UT WOS:000388493400038 ER PT J AU Hernandez-Garcia, AD Columbus, DA Manjarin, R Nguyen, HV Suryawan, A Orellana, RA Davis, TA AF Hernandez-Garcia, Adriana D. Columbus, Daniel A. Manjarin, Rodrigo Nguyen, Hanh V. Suryawan, Agus Orellana, Renan A. Davis, Teresa A. TI Leucine supplementation stimulates protein synthesis and reduces degradation signal activation in muscle of newborn pigs during acute endotoxemia SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE leucine; skeletal muscle; protein synthesis; protein degradation; autophagy ID AMINO-ACID AVAILABILITY; PEDIATRIC SEVERE SEPSIS; SKELETAL-MUSCLE; NEONATAL PIGS; TRANSLATION INITIATION; DIFFERENTIAL REGULATION; POSTABSORPTIVE RATS; MAMMALIAN TARGET; IGF-I; INSULIN AB Sepsis disrupts skeletal muscle proteostasis and mitigates the anabolic response to leucine (Leu) in muscle of mature animals. We have shown that Leu stimulates muscle protein synthesis (PS) in healthy neonatal piglets. To determine if supplemental Leu can stimulate PS and reduce protein degradation (PD) signaling in neonatal muscle during endotoxemia, overnight-fasted neonatal pigs were infused for 8 h with LPS or saline while plasma amino acids, glucose, and insulin were maintained at fasting levels during pancreatic-substrate clamps. Leu or saline was infused during the last hour. Markers of PS and PD were determined in skeletal muscle. Compared with controls, Leu increased PS in longissimus dorsi (LD), gastrocnemius, and soleus muscles. LPS decreased PS in these three muscles by 36%, 28%, and 38%, but Leu antagonized that reduction by increasing PS by 84%, 81%, and 83%, respectively, when supplemented to LPS. Leu increased eukaryotic translation initiation factor (eIF)3b-raptor interactions, eIF4E-binding protein-1, and S6 kinase 1 phosphorylation as well as eIF4E-eIF4G complex formation in LD, gastrocnemius, and soleus muscles of control and LPS-treated pigs. In LD muscle, LPS increased the light chain (LC)3-II-to-LC3 ratio and muscle-specific RING finger (MuRF-1) abundance but not atrogin-1 abundance or AMP-activated protein kinase-alpha phosphorylation. Leu supplementation to LPS-treated pigs reduced the LC3-II-to-LC3 ratio, MuRF-1 abundance, and AMP-activated protein kinase-alpha phosphorylation compared with LPS alone. In conclusion, parenteral Leu supplementation attenuates the LPS-induced reduction in PS by stimulating mammalian target of rapamycin complex 1-dependent translation and may reduce PD by attenuating autophagy-lysosome and MuRF-1 signaling in neonatal skeletal muscle. C1 [Hernandez-Garcia, Adriana D.; Columbus, Daniel A.; Manjarin, Rodrigo; Nguyen, Hanh V.; Suryawan, Agus; Orellana, Renan A.; Davis, Teresa A.] USDA ARS, Childrens Nutr Res Ctr, Houston, TX USA. [Hernandez-Garcia, Adriana D.; Orellana, Renan A.] Baylor Coll Med, Dept Pediat, Crit Care Sect, Houston, TX 77030 USA. RP Davis, TA (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institutes of Health [HD-072891, AR-044474, HD-085573]; USDA NIFA [2013-67015-20438]; USDA CRIS [6250-51000-055] FX This work is a publication of the Children's Nutrition Research Center, United States Department of Agriculture (USDA)/Agricultural Research Service, and Department of Pediatrics, Baylor College of Medicine. Financial support for this project was provided by National Institutes of Health Grants HD-072891 (to T. A. Davis), AR-044474 (to T. A. Davis), and HD-085573 (to T. A. Davis) as well as USDA NIFA 2013-67015-20438 (to T. A. Davis) and USDA CRIS 6250-51000-055 (to T. A. Davis). NR 53 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD OCT 1 PY 2016 VL 311 IS 4 BP E791 EP E801 DI 10.1152/ajpendo.00217.2016 PG 11 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA EC9IB UT WOS:000388457000015 PM 27624100 ER PT J AU Broadhurst, CL Schmidt, WF Kim, MS Nguyen, JK Qin, JW Chao, KL Bauchan, GL Shelton, DR AF Broadhurst, C. Leigh Schmidt, Walter F. Kim, Moon S. Nguyen, Julie K. Qin, Jianwei Chao, Kuanglin Bauchan, Gary L. Shelton, Daniel R. TI Continuous gradient temperature Raman spectroscopy of N-6DPA and DHA from-100 to 20 degrees C SO CHEMISTRY AND PHYSICS OF LIPIDS LA English DT Article DE Gradient temperature Raman spectroscopy; n-6DPA; DHA; n-6 Docosapentaenoic acid; Docosahexaenoic acid; Raman spectroscopy ID DOCOSAHEXAENOIC ACID; DOCOSAPENTAENOIC ACID; BRAIN-DEVELOPMENT; OLEIC-ACID; DPA N-3; OMEGA-3-FATTY-ACIDS; CHOLESTEROL; EVOLUTION; SPECTRA; RETINA AB One of the great unanswered questions with respect to biological science in general is the absolute necessity of docosahexaenoic acid (DHA, 22:6n-3) in fast signal processing tissues. N-6 docosapentaenoic acid (n-6DPA, 22:5n-6), with just one less double bond, group, is fairly abundant in terrestrial food chains yet cannot substitute for DHA. Gradient temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry (DSC) to Raman spectroscopy, providing a straightforward technique to identify molecular rearrangements that occur near and at phase transitions. Herein we apply GTRS and DSC to n-6DPA and DHA from -100 to 20 degrees C. 20 Mb three-dimensional data arrays with 0.2 degrees C increments and first/second derivatives allowed complete assignment of solid, liquid and transition state vibrational modes, including low intensity/frequency vibrations that cannot be readily analyzed with conventional Raman. N-6DPA and DHA show significant spectral changes with premelting (-33 and -60 degrees C, respectively) and melting (-27 and -44 degrees C, respectively). The CH2-(HC=CH)-CH2 moieties are not identical in the second half of the DHA and DPA structures. DPA has bending (1450 cm(-1)) over almost the entire temperature range. In contrast, DHA contains major CH2 twisting (1265 cm(-1)) with no noticeable CH2 bending, consistent with a flat helical structure with a small pitch. Further modeling of neuronal membrane phospholipids must take into account torsion present in the DHA structure, which essential in determining whether the lipid chain is configured more parallel or perpendicular to the hydrophilic head group. (C) 2016 Published by Elsevier Ireland Ltd. C1 [Broadhurst, C. Leigh; Schmidt, Walter F.; Kim, Moon S.; Nguyen, Julie K.; Qin, Jianwei; Chao, Kuanglin; Shelton, Daniel R.] USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Broadhurst, C. Leigh] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Bauchan, Gary L.] USDA ARS, Electron & Confocal Microscopy Unit, Inst Plant Sci, Beltsville, MD USA. RP Broadhurst, CL (reprint author), USDA ARS, Environm Microbiol & Food Safety Lab, Bldg 161 Rm 116,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM leigh.broadhurst@ars.usda.gov NR 33 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0009-3084 EI 1873-2941 J9 CHEM PHYS LIPIDS JI Chem. Phys. Lipids PD OCT PY 2016 VL 200 BP 1 EP 10 DI 10.1016/j.chemphyslip.2016.06.003 PG 10 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA EC5DI UT WOS:000388152900001 PM 27326703 ER PT J AU Asiimwe, P Ellsworth, PC Naranjo, SE AF Asiimwe, Peter Ellsworth, Peter C. Naranjo, Steven E. TI Natural enemy impacts on Bemisia tabaci (MEAM1) dominate plant quality effects in the cotton system SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Arthropod predators; biological control; life tables; parasitoids; plant stress; whiteflies ID BOTTOM-UP FORCES; LIFE-TABLE ANALYSIS; ARGENTIFOLII HOMOPTERA ALEYRODIDAE; CONTEMPORANEOUS MORTALITY FACTORS; TOP-DOWN CONTROL; INSECT POPULATIONS; PARASITOID WASP; RELATIVE ROLES; WATER-STRESS; KEY FACTORS AB 1. Plant quality (bottom-up effects) and natural enemies (top-down effects) affect herbivore performance. Furthermore, plant quality can also influence the impact of natural enemies. 2. Lower plant quality through reduced irrigation increased the abundance of the cryptic species from the Bemisia tabaci complex [hereafter B. tabaci Middle East Asia Minor 1 (MEAM1)], but not its natural enemies on cotton. It was therefore predicted that lower plant quality would diminish the impact of natural enemies in regulating this herbivore. 3. Over three cotton seasons, plant quality was manipulated via differential irrigation and natural enemy abundance with insecticides. Life tables were used to evaluate the impact of these factors on mortality of immature B. tabaci (MEAM1) over nine generations. 4. Mortality of B. tabaci (MEAM1) was consistently affected by natural enemies but not by plant quality. This pattern was driven by high levels of sucking predation, which was the primary (key) factor associated with changes in immature mortality across all irrigation and natural enemy treatments. Dislodgement (chewing predation and weather) and parasitism contributed as key factors in some cases. Analyses also showed that elimination of sucking predation and dislodgement would have the greatest effect on overall mortality. 5. The top-down effects of natural enemies had dominant effects on populations of B. tabaci (MEAM1) relative to the bottom-up effects of plant quality. Effects were primarily due to native generalist arthropod predators and not more host-specific aphelinid parasitoids. The findings of this study demonstrate the important role of arthropod predators in population suppression and validate the importance of conservation biological control in this system for effective pest control. C1 [Asiimwe, Peter; Ellsworth, Peter C.] Univ Arizona, Maricopa Agr Ctr, Dept Entomol, Maricopa, AZ USA. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Asiimwe, Peter] Monsanto Co, 800 N Lindbergh Blvd, St Louis, MO 63167 USA. RP Naranjo, SE (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM naranjo@ars.usda.gov OI Ellsworth, Peter/0000-0002-2485-0830 FU Maricopa Agricultural Center; Arizona Pest Management Center; USDA-National Institute for Food and Agriculture Extension IPM program; USDA-ARS; Cotton Incorporated; Arizona Cotton Growers Association; Cotton Foundations FX We thank Y. Carriere, G. Wang and R. Kaggwa for helpful comments on earlier drafts of this manuscript. Ed Martin provided guidance on establishing the irrigation regimes. M. Stefanek, V. Barkley, G. Castro, F. Bojorquez, B. Stuart, J. Trejo, A. Slade, L. Rodarte and A. Sonoqui provided expert field and laboratory technical assistance. We also appreciate the support of the Maricopa Agricultural Center, the Arizona Pest Management Center including a grant from the USDA-National Institute for Food and Agriculture Extension IPM program, the USDA-ARS, Cotton Incorporated, the Arizona Cotton Growers Association, and the Cotton Foundations. NR 91 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD OCT PY 2016 VL 41 IS 5 BP 642 EP 652 DI 10.1111/een.12340 PG 11 WC Entomology SC Entomology GA EC7HU UT WOS:000388308600016 ER PT J AU Swift, SM Rowley, DT Young, C Franks, A Hyman, P Donovan, DM AF Swift, Steven M. Rowley, D. Treva Young, Carly Franks, Ashley Hyman, Paul Donovan, David M. TI The endolysin from the Enterococcus faecalis bacteriophage VD13 and conditions stimulating its lytic activity SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE VD13; endolysin; Enterococcus faecalis; peptidoglycan hydrolase ID LEVEL GLYCOPEPTIDE RESISTANCE; COMPLETE GENOME SEQUENCE; WALL BINDING DOMAIN; CRYSTAL-STRUCTURE; CLOSTRIDIUM-PERFRINGENS; ANIMAL HUSBANDRY; FECAL SAMPLES; FAECIUM; POULTRY; LYSIS AB Bacteriophages produce endolysins (peptidoglycan hydrolases) to lyse the host cell from within and release nascent bacteriophage particles. Recombinant endolysins can lyse Gram-positive bacteria when added exogenously. As a potential alternative antimicrobial, we cloned and expressed the enterococcal VD13 bacteriophage endolysin. VD13 endolysin has a CHAP catalytic domain with 92% identity with the bacteriophage IME-EF1 endolysin. The predicted size of VD13 endolysin is similar to 27 kDa as verified by SDS-PAGE. The VD13 endolysin lyses Enterococcus faecalis strains, but not E. faecium or other non-enterococci. VD13 endolysin has activity from pH 4 to pH 8, with peak activity at pH 5, and exhibits greater activity in the presence of calcium. Optimum activity at pH 5 occurs in the absence of NaCl. VD13 endolysin, in ammonium acetate (C2H3O2NH4) calcium chloride (CaCl2) buffer pH 5, is stimulated to higher activity upon heating at temperatures up to 65 degrees C for 30 min, whereas activity is lost upon heating to 42 degrees C, in pH 7 buffer. C1 [Swift, Steven M.; Rowley, D. Treva; Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, NEA, USDA, 10300 Baltimore Ave,Bldg 230 Barc East, Beltsville, MD 20705 USA. [Young, Carly; Franks, Ashley; Hyman, Paul] Ashland Univ, Biol & Toxicol Dept, Ashland, OH 44805 USA. RP Donovan, DM (reprint author), ARS, Anim Biosci & Biotechnol Lab, NEA, USDA, 10300 Baltimore Ave,Bldg 230 Barc East, Beltsville, MD 20705 USA. EM david.donovan@ars.usda.gov FU Choose Ohio First; AU Provost Student Research grant; BARD Grant [IS-4573-12R] FX Carly Young was partly supported by Choose Ohio First funding and an AU Provost Student Research grant. D. Treva Rowley was supported by BARD Grant no. IS-4573-12R to DD. NOTE: Mention of trade names or commercial products is solely for providing specific information and does not imply recommendation or endorsement by the ARS, USDA. NR 39 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD OCT PY 2016 VL 363 IS 19 AR fnw216 DI 10.1093/femsle/fnw216 PG 8 WC Microbiology SC Microbiology GA EC8HI UT WOS:000388381800008 ER PT J AU Bowman, KD Faulkner, L Kesinger, M AF Bowman, Kim D. Faulkner, Lynn Kesinger, Mike TI New Citrus Rootstocks Released by USDA 2001-2010: Field Performance and Nursery Characteristics SO HORTSCIENCE LA English DT Article DE orange; grapefruit; mandarin; lemon; lime; huanglongbing; Citrus tristeza virus; phytophthora; diaprepes; disease; cold; cultivar; US-802; US-812; US-897; US-942 ID SINENSIS L. OSBECK; LIBERIBACTER ASIATICUS; VALENCIA ORANGE; CENTRAL FLORIDA; TOLERANCE; HYBRIDS; BLIGHT; TREE AB Four new citrus rootstocks developed by USDA, ARS and released between 2001 and 2010 have gained considerable commercial popularity in Florida and have been used for propagation of more than 2 million trees over the last 2 years. Results from three new field trials are presented comparing these rootstocks with other important rootstocks, and discussion is presented to summarize field performance in these trials and from numerous other sources. The rootstocks vary widely in their effect on tree vigor. When used as a rootstock for sweet orange, 'US-802' typically supports strong vigor and development of a large tree, 'US-812' and 'US-942' provide moderate vigor, and 'US-897' induces a relatively dwarf tree. Other characteristics and attributes of the four rootstocks are also discussed, including effects on cropping, fruit quality, disease and pest resistance, and tolerance of abiotic factors. Of special relevance, relative tolerance of these rootstocks to huanglongbing (HLB) disease can be surmised from a combination of data from several sources, with 'US-942' and 'US-802' typically providing the best yields per tree in trials containing many rootstocks and affected by HLB. 'US-812' and 'US-897' have appeared to have most utility in locations where HLB is not present or effectively managed, or where trees are being planted at high density to optimize production per unit area. To facilitate continuing expansion of commercial use, information on seed production, and the relative ease of nursery propagation is also presented. C1 [Bowman, Kim D.; Faulkner, Lynn] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Kesinger, Mike] Florida Dept Agr & Consumer Serv, DPI, Winter Haven, FL 33881 USA. RP Bowman, KD (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM kim.bowman@ars.usda.gov FU Florida Citrus Production Research Advisory Council; Florida Citrus Research and Development Foundation FX This research was supported in part by grants from the Florida Citrus Production Research Advisory Council and Florida Citrus Research and Development Foundation. NR 32 TC 0 Z9 0 U1 12 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1208 EP 1214 DI 10.21273/HORTSC10970-16 PG 7 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100001 ER PT J AU Stover, E Shatters, RG Gruber, B Kumar, P Moore, GA AF Stover, Ed Shatters, Robert G., Jr. Gruber, Barrett Kumar, Prem Moore, Gloria A. TI Influence of Photoperiod Duration and Phloem Disruption through Scoring on Growth, Disease Symptoms, and Bacterial Titer in Citrus Graft Inoculated with Candidatus Liberibacter asiaticus SO HORTSCIENCE LA English DT Article DE citrus greening; disease resistance; HLB; huanglongbing; disease tolerance; stress interaction; SPAD ID GREENING-DISEASE; ROUGH LEMON; INFECTION; GENOTYPES; THAILAND; FLORIDA AB Plants inoculated with the huanglongbing (HLB)-associated bacterium, Candidatus Liberibacter asiaticus (CLas) typically must be monitored for 8-10 months to identify differences in susceptibility between genotypes. Continuous light is reported to accelerate development of HLB symptoms and field observations suggest that trees girdled by tags or tree ties showed greater symptoms. Therefore, an experiment was conducted assessing HLB susceptibility as influenced by light/dark periods of 12 hours: 12 hours and 24 hours: 0 hours, in combination with scoring tree trunks to disrupt phloem. Sixty trees of each of three citrus genotypes ('Kuharske', previously shown to be HLB resistant; rough lemon, previously shown to be HLB tolerant; and 'Valencia', highly HLB susceptible) were bud grafted using two CLas-infected buds (rough lemon and citron) per tree on 26Mar. 2012, and were placed in controlled growth rooms (one 12 hour light: 12 hour dark and one constant light) on 4 June 2012. Ten trees of each genotype in each growth room were scored 10 cm above the soil (cutting through the bark but not the wood) with a knife on 18 July 2012 and the scoring was repeated at the same scoring wounds on 30 Aug. 2012. Trees were removed from growth rooms on 12 Dec. 2012 and subsequently maintained in a greenhouse. At two to three month intervals between June 2012 and May 2013, HLB symptoms and stem diameter at 5 cm above the soil were assessed, and three leaves per tree were collected for quantitative polymerase chain reaction (qPCR) determination of CLas titer. Six months after inoculation and 3 months following imposition of treatments, the 'Valencia' scored in the 12 hour light: 12 hour dark regime, the 'Valencia' non scored trees in 24 hours of light and the 'Kuharske' scored trees in 24 hours of light displayed higher CLas titers than most other trees. After an additional two months, both scored and non-scored trees of all three genotypes in 24 hours of light had significantly elevated CLas titers compared with trees in 12 hour light: 12 hour dark regime, but within most treatments all three genotypes had titers which were not statistically different from each other. Growth of 'Kuharske' and rough lemon was enhanced; whereas 'Valencia' growth was reduced when graft-inoculated plants were maintained in continuous light. Scoring enhanced early CLas development in 'Kuharske' when combined with continuous light, had no effect in rough lemon, and showed inconsistent effects in 'Valencia'. Although continuous lighting enhanced disease progression, it did not reveal differences in HLB susceptibility. C1 [Stover, Ed; Shatters, Robert G., Jr.] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Gruber, Barrett; Kumar, Prem] Univ Florida, Indian River Res & Educ Ctr, 2199 South Rock Rd, Ft Pierce, FL 34945 USA. [Moore, Gloria A.] Univ Florida, Dept Hort Sci, 1111 Fifield Hall,Box 110690, Gainesville, FL 32611 USA. RP Stover, E (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM ed.stover@ars.usda.gov FU USDA/APHIS FX This project was funded in part through USDA/APHIS. NR 25 TC 0 Z9 0 U1 6 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1215 EP 1219 DI 10.21273/HORTSCI11084-16 PG 5 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100002 ER PT J AU Reeve, AL Skinkis, PA Vance, AJ Lee, J Tarara, JM AF Reeve, Alison L. Skinkis, Patricia A. Vance, Amanda J. Lee, Jungmin Tarara, Julie M. TI Vineyard Floor Management Influences 'Pinot noir' Vine Growth and Productivity More than Cluster Thinning SO HORTSCIENCE LA English DT Article DE Vitis vinifera; nitrogen; solar radiation environment; cover crop; photoassimilation; cool climate viticulture ID VITIS-VINIFERA L.; REGULATED DEFICIT-IRRIGATION; SAUVIGNON BLANC GRAPEVINES; LEAF GAS-EXCHANGE; FRUIT COMPOSITION; CROP LOAD; COVER CROP; CABERNET-SAUVIGNON; WINE QUALITY; WATER-USE AB Vigor and crop level management are important practices for premium wine grape production. The implications of crop thinning 'Pinot noir'(Vitis vinifera L.) vines of varying vigor were investigated in the Willamette Valley of Oregon in 2011 to 2013 to better understand the relationship between canopy size and yield within the framework of a cool-climate, premium production wine grape vineyard. To manipulate vigor, a competitive grass cover crop (Festuca rubra L.) was grown in both (Grass), alternating (Alternate), or neither side of the flanking alleyways (Tilled). Vines within each vineyard floor treatment had two crop levels applied, including cluster thinning to one cluster per shoot (Half Crop) or no crop thinning (Full Crop). Grass treatment had reduced leaf area and leaf nitrogen (N) concentrations during all years compared with Tilled treatments. Leaf photosynthesis was also lower in Grass treatments despite more light in the canopy interior. Grass treatments had lower yield than Tilled treatments in 2 of 3 years and lower yeast assimilable nitrogen (YAN) concentrations in fruit every year. There was limited impact of floor treatments on total soluble solids (TSS) and pH. Reduced yields through cluster thinning had limited impact on vegetative growth but increased TSS and pH, in 2 of 3 years. There were few floor management by crop level interactions in any year. Grass effectively reduced vegetative growth to moderate vigor levels with cane weights between 20 and 40 g. Using a competitive grass cover crop may be an effective strategy to reduce excessive vine growth and require less labor in canopy management and crop thinning without compromising basic fruit ripeness, although YAN levels need to be monitored. C1 [Reeve, Alison L.; Skinkis, Patricia A.; Vance, Amanda J.] Oregon State Univ, Dept Hort, 4017 ALS Bldg, Corvallis, OR 97331 USA. [Lee, Jungmin] ARS, USDA, Hort Crops Res Unit Corvallis OR Worksite, 29603 U 1 Lane, Parma, ID 83660 USA. [Tarara, Julie M.] Ste Michelle Wine Estates, 660 Frontier Rd, Prosser, WA 99350 USA. RP Skinkis, PA (reprint author), Oregon State Univ, Dept Hort, 4017 ALS Bldg, Corvallis, OR 97331 USA. EM patricia.skinkis@oregonstate.edu FU Northwest Center for Small Fruits Research (NCSFR); USDA-ARS CRIS (Current Research Information System) project [2072-21000-047-00D]; Oregon Wine Board FX This research project was led by PI (Skinkis) and two sequential graduate students (Vance 2011) and Reeve (2012 onward). Project co-PIs (Tarara and Lee) provided expertise in methodology and data interpretation and partial funding. This project was supported, in part, by funds from the Northwest Center for Small Fruits Research (NCSFR), USDA-ARS CRIS (Current Research Information System) project number 2072-21000-047-00D, and the Oregon Wine Board. The authors greatly appreciate Rob Schultz, Bill Stoller, and others of Stoller Family Estate Vineyards (Dayton, OR) for their cooperation in conducting this research on-site. NR 77 TC 0 Z9 0 U1 8 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1233 EP 1244 DI 10.21273/HORTSC10998-16 PG 12 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100005 ER PT J AU Tong, CBS Chang, HY Boldt, JK Ma, YZB DeEll, JR Moran, RE Bourgeois, G Plouffe, D AF Tong, Cindy B. S. Chang, Hsueh-Yuan Boldt, Jennifer K. Ma, Yizhou B. DeEll, Jennifer R. Moran, Renae E. Bourgeois, Gaetan Plouffe, Dominique TI Diffuse Flesh Browning in 'Honeycrisp' Apple Fruit is Associated with Low Temperatures during Fruit Growth SO HORTSCIENCE LA English DT Article DE Malus xdomestica; disorder; storage; sugars; weather ID STORAGE DISORDERS; MINERAL-COMPOSITION; HARVEST MATURITY; SOFT SCALD; BITTER PIT; QUALITY; SPRAYS; ANTIOXIDANT; PHOSPHORUS; ATMOSPHERE AB Multiple types of flesh browning can occur as storage disorders in 'Honeycrisp' apple (Malus x domestica Borkh.) fruit. Predicting its occurrence is hindered by differing definitions of the types of browning, incomplete understanding of their etiologies, and difficulty in assessing harvest maturity of 'Honeycrisp' fruit. In 2013, of 'Honeycrisp' fruit grown, harvested over multiple weeks, and stored in Maine, Minnesota, Ontario, and Quebec, only the Quebec fruit developed diffuse flesh browning. A detailed comparison showed that the Quebec fruit differed in size, but not in other quality attributes, from fruit of the other locations. The Quebec fruit experienced lower temperatures during active fruit growth and were increasing in cell size up to harvest. Analyses of climate data from 2009 to 2015 indicated that accumulated growing degree-days (GDD) 50-60 day after full bloom (DAFB) could account for 31% of the variation in diffuse flesh browning, and seasonal GDD <500 are associated with a greater likelihood of injury. Fruit that exhibited diffuse flesh browning had higher magnesium and lower fructose levels than unaffected fruit. As these measurements were made after browning was assessed, the timing of the onset of these characteristics in relation to browning cannot be determined. C1 [Tong, Cindy B. S.; Chang, Hsueh-Yuan; Boldt, Jennifer K.; Ma, Yizhou B.] Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. [DeEll, Jennifer R.] Ontario Minist Agr Food & Rural Affairs, Simcoe, ON N3Y 4N5, Canada. [Moran, Renae E.] Univ Maine, Dept Plant Soil & Environm Sci, POB 179, Monmouth, ME 04259 USA. [Bourgeois, Gaetan; Plouffe, Dominique] Agr & Agri Food Canada, Hort Res & Dev Ctr, 430 Blvd Gouin, St Jean, PQ J3B 3E6, Canada. [Boldt, Jennifer K.] ARS, USDA, Greenhouse Prod Res Grp, 2801 W Bancroft St,Mail Stop 604, Toledo, OH 43606 USA. RP Tong, CBS (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM c-tong@umn.edu FU Minnesota Agricultural Experiment Station for MN [21-065]; University of Minnesota Undergraduate Research Opportunity Program; Maine Agricultural and Forest Experiment Station Project [ME031404] FX This project received funding from the Minnesota Agricultural Experiment Station for MN 21-065, University of Minnesota Undergraduate Research Opportunity Program, and Maine Agricultural and Forest Experiment Station Project ME031404. NR 33 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1256 EP 1264 DI 10.21273/HORTSCI11179-16 PG 9 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100008 ER PT J AU Shock, CC Feibert, EBG Rivera, A Saunders, LD Shaw, N Kilkenny, FF AF Shock, Clinton C. Feibert, Erik B. G. Rivera, Alicia Saunders, Lamont D. Shaw, Nancy Kilkenny, Francis F. TI Irrigation Requirements for Seed Production of Five Lomatium Species in a Semiarid Environment SO HORTSCIENCE LA English DT Article DE subsurface drip irrigation; medicinal plant; rangeland restoration ID FERTILIZATION; ESTABLISHMENT; DISSECTUM; GROWTH; OREGON AB Seeds of native plants are needed for rangeland restoration in the Intermountain West. Many of these plants are rarely cultivated and relatively little is known about the cultural practices required for their seed production. Irrigation trials were conducted for five perennial Lomatium species over multiple years. Lomatium species grown at the Oregon State University Malheur Experiment Station, Ontario, OR received 0, 100, or 200 mm of irrigation per year. Seed yield responses to irrigation were evaluated by linear and quadratic regression. In general, seed yields from the three species grown for 10 years responded linearly or quadratically to irrigation. To improve the accuracy of estimated irrigation water requirements, regressions were also run on seed yield responses to irrigation plus precipitation during the previous spring; spring and winter; and spring, winter, and fall. Over multiple years, Lomatium dissectum (Nutt.) Mathias & Constance and L. triternatum (Pursh) J.M. Coult. & Rose seed yields were best estimated by a quadratic response to irrigation plus spring precipitation with highest yields at 243 and 255 mm, respectively. Lomatium grayi (J.M. Coult. & Rose) J.M. Coult. & Rose seed yields were best estimated by a quadratic response to irrigation plus precipitation during the fall, winter, and spring with highest yields at 358 mm. Two of the Lomatium species were grown for the last 6 years. The seed yields of L. nudicaule (Pursh) J.M. Coult. & Rose did not respond to irrigation. Seed yields of Lomatium suksdorfii (S. Watson) J.M. Coult. & Rose responded linearly to irrigation in 2015. C1 [Shock, Clinton C.; Feibert, Erik B. G.; Rivera, Alicia; Saunders, Lamont D.] Oregon State Univ, Malheur Expt Stn, 595 Onion Ave, Ontario, OR 97914 USA. [Shaw, Nancy; Kilkenny, Francis F.] US Forest Serv, Rocky Mt Res Stn, 322 E Front St,Suite 401, Boise, ID 83702 USA. RP Shock, CC (reprint author), Oregon State Univ, Malheur Expt Stn, 595 Onion Ave, Ontario, OR 97914 USA. EM clinton.shock@oregonstate.edu FU USDA Forest Service; Rocky Mountain Research Station's Great Basin Native Plant Project; USDI Bureau of Land Management; Oregon State University; Malheur County Education Service District; USDA National Institute of Food and Agriculture [2015-31100-06041, 2015-31200-06041] FX This project was partially funded by the USDA Forest Service, Rocky Mountain Research Station's Great Basin Native Plant Project, USDI Bureau of Land Management, Oregon State University, Malheur County Education Service District, and by formula grants 2015-31100-06041 and 2015-31200-06041 from the USDA National Institute of Food and Agriculture. NR 21 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1270 EP 1277 DI 10.21273/HORTSCI11150-16 PG 8 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100010 ER PT J AU Hugie, KL Watkins, E AF Hugie, Kari L. Watkins, Eric TI Performance of Low-input Turfgrass Species as Affected by Mowing and Nitrogen Fertilization in Minnesota SO HORTSCIENCE LA English DT Article DE hard fescue; prairie junegrass; tufted hairgrass; colonial bentgrass; nitrogen fertilizer; mowing ID JUNEGRASS GERMPLASM ACCESSIONS; MANAGEMENT; QUALITY; GRASSES; RESPONSES AB In Minnesota, most lawns and higher cut turfgrass areas consist primarily of species such as kentucky bluegrass (Poa pratensis L.) and perennial ryegrass (Lolium perenne L.) that require significant management inputs such as frequent mowing and nitrogen fertility. Several studies have shown that other species have the potential to be used more widely on home lawns in Minnesota; however, little is known about the management requirements of these species. In this study, we evaluated the performance of several alternative grass species under varying mowing and nitrogen fertility regimes at two sites in Minnesota in 2010 and 2011. Hard fescue [Festuca trachyphylla (Hackel) Krajina] showed the most consistent performance across management regimes, seasons, and locations. Colonial bentgrass (Agrostis tenuis Sibth.) showed good spring and fall turf quality, but suffered from excess thatch development and disease incidence. 'Barkoel' prairie junegrass [Koeleria macrantha (Ledeb.) Schult] maintained acceptable turf cover throughout the trial, whereas unimproved native prairie junegrass populations did poorly regardless of management level. Tufted hairgrass [Deschampsia cespitosa (L.) P. Beauv.] did not perform consistently in the trial due to summer stress. Our results show that hard fescue, colonial bentgrass, and 'Barkoel' prairie junegrass performed well regardless of mowing height or fertility treatment and could be used to a greater degree as low-input turfgrasses in Minnesota. C1 [Hugie, Kari L.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC 29501 USA. [Watkins, Eric] Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. RP Watkins, E (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM ewatkins@umn.edu FU Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture [2009-34381-20044] FX This material is based on research supported by the Cooperative State Research, Education, and Extension Service, U.S. Department of Agriculture, under agreement no. 2009-34381-20044. NR 31 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD OCT PY 2016 VL 51 IS 10 BP 1278 EP 1286 DI 10.21273/HORTSCI11007-16 PG 9 WC Horticulture SC Agriculture GA EC5NZ UT WOS:000388184100011 ER PT J AU Miao, H Zhang, SP Wang, M Wang, Y Weng, YQ Gu, XF AF Miao, Han Zhang, Shengping Wang, Min Wang, Ye Weng, Yiqun Gu, Xingfang TI Fine Mapping of Virescent Leaf Gene v-1 in Cucumber (Cucumis sativus L.) SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE cucumber; virescent leaf; chloroplast development; map-based cloning ID BURROWS-WHEELER TRANSFORM; ARABIDOPSIS-THALIANA; CHLOROPLAST DEVELOPMENT; CHLOROPHYLL BIOSYNTHESIS; MUTANT REVEALS; NUCLEAR GENE; RICE; PROTEIN; GENOME; WHEAT AB Leaf color mutants are common in higher plants that can be used as markers in crop breeding or as an important tool in understanding regulatory mechanisms in chlorophyll biosynthesis and chloroplast development. In virescent leaf mutants, young leaves are yellow in color, which gradually return to normal green when the seedlings grow large. In the present study, we conducted phenotypic characterization and genetic mapping of the cucumber virescent leaf mutant 9110Gt conferred by the v-1 locus. Total chlorophyll and carotenoid content in 9110Gt was reduced by 44% and 21%, respectively, as compared with its wild type parental line 9110G. Electron microscopic investigation revealed fewer chloroplasts per cell and thylakoids per chloroplast in 9110Gt than in 9110G. Fine genetic mapping allowed for the assignment of the v-1 locus to a 50.4 kb genomic DNA region in chromosome 6 with two flanking markers that were 0.14 and 0.16 cM away from v-1, respectively. Multiple lines of evidence supported CsaCNGCs as the only candidate gene for the v-1 locus, which encoded a cyclic-nucleotide-gated ion channel protein. A single nucleotide change in the promoter region of v-1 seemed to be associated with the virescent color change in 9110Gt. Real-time PCR revealed significantly lower expression of CsaCNGCs in the true leaves of 9110Gt than in 9110G. This was the first report that connected the CsaCNGCs gene to virescent leaf color change, which provided a useful tool to establish linkages among virescent leaf color change, chloroplast development, chlorophyll biosynthesis, and the functions of the CsaCNGCs gene. C1 [Miao, Han; Zhang, Shengping; Wang, Min; Wang, Ye; Gu, Xingfang] Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China. [Miao, Han; Weng, Yiqun] Univ Wisconsin, Dept Hort, USDA ARS Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Gu, XF (reprint author), Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China.; Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, USDA ARS Vegetable Crops Res Unit, Madison, WI 53706 USA. EM miaohan@caas.cn; zhangshengping@caas.cn; w.jsun@163.com; wangye@caas.cn; yiqun.weng@ars.usda.gov; guxingfang@mail.caas.cn FU China Scholarship Council; Natural Science Foundation of China [31101549]; China Agriculture Research System [CARS-25]; Science and Technology Innovation Program of the Chinese Academy of Agricultural Science (CAAS-ASTIP-IVFCAAS); Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China FX The authors thank Caiqing Xu and Kristin Haider for technical assistance and Graham Collins (Roseworthy Campus of the University of Adelaide in South Australia) for revision of the manuscript. HM's work in YW's laboratory was partially funded by the China Scholarship Council. This research was supported by the Natural Science Foundation of China (31101549), China Agriculture Research System (CARS-25), Science and Technology Innovation Program of the Chinese Academy of Agricultural Science (CAAS-ASTIP-IVFCAAS), and the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China. NR 66 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD OCT PY 2016 VL 17 IS 10 AR 1602 DI 10.3390/ijms17101602 PG 12 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA EC0DO UT WOS:000387768300182 ER PT J AU Rinella, MJ Espeland, EK Moffatt, BJ AF Rinella, Matthew J. Espeland, Erin K. Moffatt, Bruce J. TI Studying long-term, large-scale grassland restoration outcomes to improve seeding methods and reveal knowledge gaps SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE adaptive management; Agropvron cristatum; Bromus tectorum; compositional data analysis; crested wheatgrass; Great Plains; rangeland; reclamation; seed rate; shrubs ID ADAPTIVE-MANAGEMENT; PERENNIAL GRASSES; PLANT-COMMUNITIES; ECOLOGICAL RESTORATION; POPULATION-DYNAMICS; MODELING APPROACH; COMPETITION; ESTABLISHMENT; RANGELAND; FERTILIZATION AB 1. Studies are increasingly investigating effects of large-scale management activities on grassland restoration outcomes. These studies are providing useful comparisons among currently used management strategies, but not the novel strategies needed to rapidly improve restoration efforts. Here we illustrate how managing restoration projects adaptively can allow promising management innovations to be identified and tested. 2. We studied 327 Great Plains fields seeded after coal mining. We modelled plant responses to management strategies to identify the most effective previously used strategies for constraining weeds and establishing desired plants. Then, we used the model to predict responses to new strategies our analysis identified as potentially more effective. 3. Where established, the weed crested wheatgrass (Agropyron cristatum L.) increased through time, indicating a need to manage establishment of this grass. Seeding particular grasses reduced annual weed cover, and because these grasses appeared to become similarly abundant whether sown at low or high rates, low rates could likely be safely used to reduce seeding costs. More importantly, lower than average grass seed rates increased cover of shrubs, the plants most difficult to restore to many grassland ecosystems. After identifying grass seed rates as a driver, we formulated model predictions for rates below the range managers typically use. These predictions require testing but indicated atypically low grass seed rates would further increase shrubs without hindering long-term grass stand development. 4. Synthesis and applications. Designing management around empirically based predictions is a logical next step towards improving ecological restoration efforts. Our predictions are that reducing grass seed rates to atypically low levels will boost shrubs without compromising grasses. Because these predictions derive from the fitted model, they represent quantitative hypotheses based on current understanding of the system. Generating data needed to test and update these hypotheses will require monitoring responses to shifts in management, specifically shifts to lower grass seed rates. A paucity of data for confronting hypotheses has been a major sticking point hindering adaptive management of most natural resources, but this need not he the case with degraded grasslands, because ongoing restoration efforts around the globe are providing continuous opportunities to monitor and manage processes regulating grassland restoration outcomes. C1 [Rinella, Matthew J.] USDA ARS, Rangeland Ecol, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. [Espeland, Erin K.] USDA ARS, Pest Management Unit, Northern Plains Agr Res Lab, Sidney, MT USA. [Moffatt, Bruce J.] Oregon State Univ, Dept Soil Sci, Corvallis, OR 97331 USA. RP Rinella, MJ (reprint author), USDA ARS, Rangeland Ecol, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM Matt.Rinella@ars.usda.gov NR 66 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2016 VL 53 IS 5 BP 1565 EP 1574 DI 10.1111/1365-2664.12722 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0FE UT WOS:000387773000028 ER PT J AU Livingston, AC Varner, JM Jules, ES Kane, JM Arguello, LA AF Livingston, Amy C. Varner, J. Morgan Jules, Erik S. Kane, Jeffrey M. Arguello, Leonel A. TI Prescribed fire and conifer removal promote positive understorey vegetation responses in oak woodlands SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE conifer encroachment; ecological restoration; non-metric multidimensional scaling; non-native species; oak woodlands; Oregon white oak; prescribed fire; Pseudotsuga menziesii; Quercus garryana; understorey vegetation ID WOODY PLANT ENCROACHMENT; PACIFIC-NORTHWEST; SPECIES RICHNESS; MESIC GRASSLAND; WESTERN OREGON; FORT-LEWIS; SAVANNA; WASHINGTON; PRAIRIES; FOREST AB 1. Fire-prone woodlands and savannas world-wide face management challenges resulting from fire exclusion and subsequent encroachment of fire sensitivetrees. In the Pacific Northwest (USA), Quercus garryana oak woodlands and savannas are threatened by encroachment from the native conifer Pseudotsuga menziesii in the absence of fire. 2. In the Bald Hills of Redwood National Park (California, USA), prescribed fire and conifer removal have been used to restore encroached woodlands. We examined the effects of encroachment and restoration on understorey vegetation, comparing four treatments: prescribed fire, prescribed fire and conifer removal, conifer removal, and encroached (control). 3. Treatments including prescribed fire had the greatest native species richness. These two treatments also had the greatest non-native species richness, at both the site level and the treatment level. Woodlands treated with conifer removal and no prescribed lire were intermediate in species richness and diversity compared to burned treatments and encroached woodlands. Encroached woodlands had diminished richness and diversity compared to all restoration treatments. Non-metric multidimensional scaling (NMS) ordination demonstrated that conifer basal area, conifer litter and fine wood were associated with low species richness and diversity and that elevation and thatch were associated with higher species richness and diversity. Indicator species analysis identified that most native species and non-native species were associated with burned woodlands that were never encroached. 4. Synthesis and applications. Our results suggest that both prescribed fire and conifer removal have benefits for understorey plant communities, increasing species richness, diversity and cover in oak woodlands and shifting understorey communities from forest-associated species to more woodland-associated species. Restoration of remnant Quercus garryana oak woodlands is complicated by the persistence and abundance of non-native herbaceous plants. C1 [Livingston, Amy C.; Varner, J. Morgan; Kane, Jeffrey M.] Humboldt State Univ, Dept Forestry & Wildland Resources, Wildland Fire Lab, Arcata, CA 95521 USA. [Varner, J. Morgan] US Forest Serv, USDA, Pacific Northwest Res Stn, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. [Jules, Erik S.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. [Arguello, Leonel A.] Redwood Natl Park, Orick, CA 95555 USA. RP Livingston, AC (reprint author), Trinity Cty Resource Conservat Dist, POB 1450, Weaverville, CA 96093 USA. EM acl15@humboldt.edu FU USDA McIntire-Stennis Program; University of California Agriculture and Natural Resources Program FX Funding was provided by the USDA McIntire-Stennis Program and the University of California Agriculture and Natural Resources Program. Advice on statistical analysis was provided by R. Van Kirk. Discussions with E. Engber, S. Samuels, J. McClelland, E. Asarian and J. Deshais improved this work. K. Eldridge, P. Nguyen, A. Lottes, N. Hancock, E. Nichols, A. Shackelford, K. Belief, A. Geritz, L. Lascheck and M. Schriver assisted with data collection. The paper benefitted from thoughtful comments and criticisms provided by anonymous reviewers. NR 60 TC 0 Z9 0 U1 19 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD OCT PY 2016 VL 53 IS 5 BP 1604 EP 1612 DI 10.1111/1365-2664.12703 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EC0FE UT WOS:000387773000032 ER PT J AU Cameron, RG Chau, HK Manthey, JA AF Cameron, Randall G. Chau, Hoa K. Manthey, John A. TI Continuous process for enhanced release and recovery of pectic hydrocolloids and phenolics from citrus biomass SO JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY LA English DT Article DE citrus; pectin; phenolics; steam-explosion; agricultural waste valorization ID SUPERCRITICAL-FLUID EXTRACTION; SIMULTANEOUS SACCHARIFICATION; ORANGE PEEL; WASTE; ACID; FERMENTATION; PRETREATMENT; LIMONOIDS; ETHANOL AB BACKGROUND: During the 2012/2013 harvest season the Florida citrus juice industry produced 0.55x10(6) metric tons (MT) of dried citrus pellets from citrus processing waste (CPW). The citrus pellets were marketed as a low value animal feed which typically sold for $0.22 per kg or less. Yet, this biomass also contained nearly 110 000MT of pectic hydrocolloids and 25 000MT of flavonoids and related phenolics as potential value-added co-products. A continuous process was explored to release and recover these co-products in a manner that enables their desired functionalities. RESULTS: Injecting steam into the flow of citrus peel through a pipe at 150 degrees C released pectic hydrocolloids and phenolic compounds, as well as several other classes of secondary metabolites from the waste biomass. The resulting steam-exploded peel was washed with water and pectic hydrocolloids and several major classes of phenolic compounds were recovered in the wash water. Over two seasons an average of 72% of the pectic hydrocolloids were recovered. In addition, 41.1% of the polymethoxylated flavones, 11.4% of the flavanone glycosides, 85.2% of the limonoids and nearly 100% of hydroxycinnamates were recovered with the water wash. CONCLUSION: The continuous steam treatment of citrus processing waste provides an enhanced, environmentally friendly method for release and recovery of valuable pectic hydrocolloids and phenolic compounds. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Cameron, Randall G.; Manthey, John A.] ARS, USDA, US Hort Res Lab, Citrus & Other Subtrop Prod Res Unit, Ft Pierce, FL 34952 USA. [Chau, Hoa K.] ARS, USDA, Eastern Reg Res Ctr, Dairy & Funct Foods Res Unit, Wyndmoor, PA 19038 USA. RP Cameron, RG (reprint author), ARS, USDA, US Hort Res Lab, Citrus & Other Subtrop Prod Res Unit, Ft Pierce, FL 34952 USA. EM Randall.cameron@ars.usda.gov NR 42 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-2575 EI 1097-4660 J9 J CHEM TECHNOL BIOT JI J. Chem. Technol. Biotechnol. PD OCT PY 2016 VL 91 IS 10 BP 2597 EP 2606 DI 10.1002/jctb.4854 PG 10 WC Biotechnology & Applied Microbiology; Chemistry, Multidisciplinary; Engineering, Environmental; Engineering, Chemical SC Biotechnology & Applied Microbiology; Chemistry; Engineering GA EC7BX UT WOS:000388291900006 ER PT J AU Castro, P Lewers, KS AF Castro, P. Lewers, K. S. TI Identification of quantitative trait loci (QTL) for fruit-quality traits and number of weeks of flowering in the cultivated strawberry SO MOLECULAR BREEDING LA English DT Article DE Fragaria x ananassa; Flowering habit; Fruit-quality traits; QTL analysis; Molecular markers ID OCTOPLOID FRAGARIA-VIRGINIANA; ASCORBIC-ACID CONTENT; X-ANANASSA DUCH; DIPLOID FRAGARIA; DAY-NEUTRALITY; LINKAGE MAPS; XANANASSA DUCH.; SEX-CHROMOSOME; HUMAN HEALTH; INHERITANCE AB Fruit quality and repeat flowering are two major foci of several strawberry breeding programs. The identification of quantitative trait loci (QTL) and molecular markers linked to these traits could improve breeding efficiency. In this work, an F1 population derived from the cross 'Delmarvel' 9 'Selva' was used to develop a genetic linkage map for QTL analyses of fruit-quality traits and number of weeks of flowering. Some QTL for fruit-quality traits were identified on the same homoeologous groups found in previous studies, supporting trait association in multiple genetic backgrounds and utility in multiple breeding programs. None of the QTL for soluble solids colocated with a QTL for titratable acids, and, although the total soluble solid contents were significantly and positively correlated with titratable acids, the correlation coefficient value of 0.2452 and independence of QTL indicate that selection for high soluble solids can be practiced independently of selection for low acidity. One genomic region associated with the total number of weeks of flowering was identified quantitatively on LG IV-S-1. The most significant marker, FxaACAO2I8C-145S, explained 43.3 % of the phenotypic variation. The repeat-flowering trait, scored qualitatively, mapped to the same region as the QTL. Dominance of the repeat-flowering allele was demonstrated by the determination that the repeat-flowering parent was heterozygous. This genomic region appears to be the same region identified in multiple mapping populations and testing environments. Markers linked in multiple populations and testing environments to fruit-quality traits and repeat flowering should be tested widely for use in marker-assisted breeding. C1 [Castro, P.; Lewers, K. S.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Bldg 010A BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Lewers, KS (reprint author), ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Bldg 010A BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Kim.Lewers@ars.usda.gov FU USDA-ARS [8042-21220-254-00] FX This project was funded by USDA-ARS Project 8042-21220-254-00. The authors wish to thank Drs. Stan Hokanson and Sarah Nourse who generated the population before moving on to other research opportunities, Dr. Show Wang who collected the anthocyanin, phenolics and ORAC data before retiring and passing on, and the many former USDA-ARS technicians and undergraduate students who worked on this population at some point while associated with the project. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture or any of the other agencies involved in this research. NR 69 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD OCT PY 2016 VL 36 IS 10 AR 138 DI 10.1007/s11032-016-0559-7 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA EC5YA UT WOS:000388212000003 ER PT J AU Pankratov, I McQuinn, R Schwartz, J Bar, E Fei, ZJ Lewinsohn, E Zamir, D Giovannoni, JJ Hirschberg, J AF Pankratov, Ilya McQuinn, Ryan Schwartz, Jochanan Bar, Einat Fei, Zhangjun Lewinsohn, Efraim Zamir, Dani Giovannoni, James J. Hirschberg, Joseph TI Fruit carotenoid-deficient mutants in tomato reveal a function of the plastidial isopentenyl diphosphate isomerase (IDI1) in carotenoid biosynthesis SO PLANT JOURNAL LA English DT Article DE isoprenoid biosynthesis; carotenoids; fruit development; Solanum lycopersicum; plastid; de-etiolation ID METHYLERYTHRITOL PHOSPHATE-PATHWAY; ISOPRENOID BIOSYNTHESIS; ARABIDOPSIS-THALIANA; PHYTOENE SYNTHASE; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; MEVALONATE PATHWAY; HIGHER-PLANTS; SWEET BASIL; GENE AB Isoprenoids consist of a large class of compounds that are present in all living organisms. They are derived from the 5C building blocks isopentenyl diphosphate (IDP) and its isomer dimethylallyl diphosphate (DMADP). In plants, IDP is synthesized in the cytoplasm from mevalonic acid via the MVA pathway, and in plastids from 2-C-methyl-D-erythritol-4-phosphate through the MEP pathway. The enzyme IDP isomerase (IDI) catalyzes the interconversion between IDP and DMADP. Most plants contain two IDI enzymes, the functions of which are characteristically compartmentalized in the cells. Carotenoids are isoprenoids that play essential roles in photosynthesis and provide colors to flowers and fruits. They are synthesized in the plastids via the MEP pathway. Fruits of Solanum lycopersicum (tomato) accumulate high levels of the red carotene lycopene. We have identified mutations in tomato that reduce overall carotenoid accumulation in fruits. Four alleles of a locus named FRUIT CAROTENOID DEFICIENT 1 (fcd1) were characterized. Map-based cloning of fcd1 indicated that this gene encodes the plastidial enzyme IDI1. Lack of IDI1 reduced the concentration of carotenoids in fruits, flowers and cotyledons, but not in mature leaves. These results indicate that the plastidial IDI plays an important function in carotenoid biosynthesis, thus highlighting its role in optimizing the ratio between IDP and DMADP as precursors for different downstream isoprenoid pathways. C1 [Pankratov, Ilya; Schwartz, Jochanan; Hirschberg, Joseph] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel. [McQuinn, Ryan; Fei, Zhangjun; Giovannoni, James J.] Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [McQuinn, Ryan; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Bar, Einat; Lewinsohn, Efraim] Agr Res Org, Inst Field & Vegetable Crops, Newe Yaar Res Ctr, POB 1021, Ramat Yishay, Israel. [Zamir, Dani] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet, Fac Agr, IL-91904 Jerusalem, Israel. RP Hirschberg, J (reprint author), Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel. EM hirschu@mail.huji.ac.il FU Israel Science Foundation [1685/09]; EU-FP7 METAPRO [244348] FX This research was supported by grants 1685/09, from the Israel Science Foundation, and EU-FP7 METAPRO 244348. NR 72 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD OCT PY 2016 VL 88 IS 1 BP 82 EP 94 DI 10.1111/tpj.13232 PG 13 WC Plant Sciences SC Plant Sciences GA EC9CS UT WOS:000388442100007 PM 27288653 ER PT J AU Schreiner, RP AF Schreiner, R. Paul TI Nutrient Uptake and Distribution in Young Pinot noir Grapevines over Two Seasons SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE biomass; micronutrients; nitrogen; nutrient reserves; phosphorus; potassium ID THOMPSON SEEDLESS GRAPEVINES; VITIS-VINIFERA L.; DRY-MATTER; ACCUMULATION; NITROGEN; GROWTH; EFFICIENCY; BERRIES; ROOTS; YIELD AB The seasonal timing of biomass and nutrient distribution among different vine organs was determined over two growing seasons in four-year-old Pinot noir grapevines grown in field microplots. Vines were fertilized in spring, and biomass and nutrient contents of nine separate vine parts were measured at six phenological stages (budbreak, bloom, veraison, harvest, leaf fall, and dormancy) each year. The uptake and distribution of four micronutrients-boron (B), zinc (Zn), Manganese (Mn), and copper (Cu)-was determined for the first time in field-grown grapevines. Whole-vine nitrogen (N) uptake was maximal early in the season, with most N uptake occurring before bloom. Uptake of phosphorus (P) and sulfur (S) was also early compared to other nutrients, with similar quantities of these elements taken up between budbreak and bloom and between bloom and veraison. All other macronutrients-potassium (K), calcium (Ca), and magnesium (Mg)-and micronutrients (B, Zn, Mn, Cu) had peak uptake between bloom and veraison. Remobilization of nutrients from permanent vine structures helped supply early season canopy needs for N, K, and S. More N was remobilized from reserves and this lasted until veraison, supplying similar to 35% of the canopy N between budbreak and veraison. Remobilization of K and S occurred only until bloom and contributed similar to 30% of the canopy increase in these elements up to that time. The small root fraction was the main source of remobilized nutrients for N, K, and S. A net annual gain of nutrients in the permanent vine parts occurred for P, K, Ca, Mg, Zn, and Cu. The quantities of each nutrient required by young Pinot noir grapevines carrying a typical crop yield for the region are presented and discussed. C1 [Schreiner, R. Paul] ARS, USDA, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Schreiner, RP (reprint author), ARS, USDA, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM paul.schreiner@ars.usda.gov NR 31 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD OCT PY 2016 VL 67 IS 4 BP 436 EP 448 DI 10.5344/ajev.2016.16019 PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA EC4KF UT WOS:000388097000009 ER PT J AU Tognella, MMP Soares, MLG Cuevas, E Medina, E AF Tognella, M. M. P. Soares, M. L. G. Cuevas, E. Medina, E. TI Heterogeneity of elemental composition and natural abundance of stables isotopes of C and N in soils and leaves of mangroves at their southernmost West Atlantic range SO BRAZILIAN JOURNAL OF BIOLOGY LA English DT Article DE mangroves; soils; leaves; nutrients; stable isotopes; southwestern Atlantic latitudinal limit ID RHIZOPHORA-MANGLE; NUTRIENT RELATIONS; ORGANIC-CARBON; RIVER ESTUARY; SEPETIBA BAY; HEAVY-METALS; PUERTO-RICO; VARIABILITY; FORESTS; FLORIDA AB Mangrove communities were selected in the state of Santa Catarina, Brazil, near their southernmost limit of distribution, to study mineral nutrient relation in soils and plants. Communities included three true mangrove species, Rhizophora mangle, Laguncularia racemosa and Avicennia germinans, and two associated species, the fern Acrostichum danaeifolium, and the grass Spartina densiflora. The sites included communities in the lower Rio Tavares near Florianopolis city, Sonho beach near Palhoca city, and the Santo Antonio lagoon. These sites included a full range of mangroves under humid climate where winter temperatures, instead of salinity, may be the main factor regulating their productive capacity and species composition. Soil salinity was determined by the concentration of soluble Na, and soil C and N were linearly correlated indicating their association in organic matter. Tavares site showed higher specific conductivity, and concentrations of Na and Mg in the soil layer below 40 cm depth, indicating larger influence of marine water. Isotopic signature of C increased with soil depth suggesting that microorganisms decomposing organic matter are releasing C-13 depleted CO2. Nitrogen isotopic signature decreased with soil depth, indicating enrichment in N-15 possibly as a result of denitrification in the upper soil layers. Mineral elements in leaf tissues showed A. schaueriana with higher concentrations of N, P, Na, K, Cu, Zn, and Na/Ca ratio. Spartina densiflora was characterized by the lowest N and K concentrations, and the highest concentrations of A1 and Fe. Rhizophora mangle and L. racemosa had the highest Ca concentrations. Carbon isotopic signatures identified S. densiflora as a C4 plant, and A. schaueriana as the mangrove species occupying comparatively more water stressed microsites than the rest. Leaf nitrogen isotopic signatures were positive, in correspondence with the soil values. The results support the hypothesis that sites sampled were comparatively fertile, because sediment transport through fresh water run-off is predominant in humid coasts, and therefore plants were not limited by nutrient supply, nor particularly stressed by soil salinity. C1 [Tognella, M. M. P.] UFES, Ctr Univ Norte Espirito Santo CEUNES, Dept Ciencias Agr & Biol, Lab Gestao Manguezal GEMA, Rod BR 101 Norte,Km 60, BR-29934430 Sao Mateus, ES, Brazil. [Soares, M. L. G.] Univ Estado Rio De Janeiro, Nucleo Estudos Manguezais NEMA, Dept Oceanog Biol, Fac Oceanog FAOC, Rua Sao Francisco Xavier,524,Sala 4023-E, BR-22420020 Rio De Janeiro, RJ, Brazil. [Cuevas, E.] Univ Puerto Rico UPR Rio Piedras, Dept Biol, Fac Nat Sci, Ctr Appl Trop Ecol & Conservat, Facundo Bueso Bldg,301-A,POB 23360, San Juan, PR 00931 USA. [Medina, E.] IVIC, Ctr Ecol, Carretera Panamer,Km 11,Edo Miranda,Aptdo 20632, Caracas 1020A, Venezuela. [Medina, E.] USDA Forest Serv, Int Inst Trop Forestry, 1201,Calle Ceiba,Jardin Bot Sur, San Juan, PR 00926 USA. RP Medina, E (reprint author), IVIC, Ctr Ecol, Carretera Panamer,Km 11,Edo Miranda,Aptdo 20632, Caracas 1020A, Venezuela.; Medina, E (reprint author), USDA Forest Serv, Int Inst Trop Forestry, 1201,Calle Ceiba,Jardin Bot Sur, San Juan, PR 00926 USA. EM medinage@gmail.com NR 41 TC 0 Z9 0 U1 13 U2 13 PU INT INST ECOLOGY PI SAO CARLOS PA RUA BENTO CARLOS, 750 - CENTRO, SAO CARLOS, SP 00000, BRAZIL SN 1519-6984 EI 1678-4375 J9 BRAZ J BIOL JI Braz. J. Biol. PD OCT-DEC PY 2016 VL 76 IS 4 BP 994 EP 1003 DI 10.1590/1519-6984.05915 PG 10 WC Biology SC Life Sciences & Biomedicine - Other Topics GA EB8QU UT WOS:000387656700024 PM 27191466 ER PT J AU Santiago, S Roll, DM Ray, C Williams, C Moravcik, P Knopf, A AF Santiago, Sergio Roll, Deborah M. Ray, Chittaranjan Williams, Clinton Moravcik, Philip Knopf, Allan TI Effects of soil moisture depletion on vegetable crop uptake of pharmaceuticals and personal care products (PPCPs) SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Pharmaceuticals and personal care products; PPCPs; Soil moisture depletion; Crop uptake ID WATER TREATMENT PLANTS; ENDOCRINE DISRUPTING COMPOUNDS; ANTIBIOTIC-RESISTANCE GENES; SEWAGE-TREATMENT PLANT; MUNICIPAL WASTE-WATER; RECYCLED WATER; ORGANIC-COMPOUNDS; TERRESTRIAL ENVIRONMENT; AQUATIC CONTAMINATION; RECLAIMED WATER AB Agricultural crops have a long history of being irrigated with recycled wastewater (RW). However, its use on vegetable crops has been of concern due to the potential prevalence of microcontaminants, such as pharmaceuticals and personal care products (PPCPs) in the latter, which represents a possible health hazard to consumers. We investigated the uptake of three PPCPs (atenolol, diclofenac, and ofloxacin), at three different concentrations in irrigation water (0.5, 5, and 25 mu g L-1) in relation to three varying volumetric soil moisture depletion levels of 14 % (-4.26 kPa), 10 % (-8.66 kPa), and 7 % (-18.37 kPa) by various vegetable crop species. Experiments were conducted in a split-split block completely randomized design. PPCPs were extracted using a developed method of accelerated solvent extraction and solid phase extraction and analyzed via liquid chromatography mass spectrometry (LCMS). Results indicate that all treated crops were capable of PPCP uptake at nanogram per gram concentrations independent of the applied soil moisture depletion levels and PPCP concentrations. Ofloxacin was the chemical with the highest uptake amounts, followed by atenolol and then diclofenac. Although the results were not statistically significant, higher concentrations of PPCPs were detected in plants maintained under higher soil moisture levels of 14 % (-4.26 kPa). C1 [Santiago, Sergio; Ray, Chittaranjan] Univ Hawaii, Dept Civil & Environm Engn, Honolulu, HI 96822 USA. [Santiago, Sergio; Ray, Chittaranjan; Moravcik, Philip] Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA. [Roll, Deborah M.; Williams, Clinton; Knopf, Allan] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Ray, Chittaranjan] Nebraska Water Ctr, Nebraska Innovat Campus, Lincoln, NE 68588 USA. RP Ray, C (reprint author), Univ Hawaii, Dept Civil & Environm Engn, Honolulu, HI 96822 USA.; Ray, C (reprint author), Univ Hawaii, Water Resources Res Ctr, Honolulu, HI 96822 USA.; Ray, C (reprint author), Nebraska Water Ctr, Nebraska Innovat Campus, Lincoln, NE 68588 USA. EM cray@nebraska.edu FU US Department of Agriculture (USDA) Agriculture and Food Research Initiative (AFRI) Grant Accession [224076, HAWR-2010-03561] FX This study was funded by the US Department of Agriculture (USDA) Agriculture and Food Research Initiative (AFRI) Grant Accession No. 224076 (Project No. HAWR-2010-03561) and received institutional support from the University of Hawaii at Manoa, College of Tropical Agriculture and Human Resources (CTAHR). The authors would like to thank Travis Idol, Kheng Cheah, Theodore Radovich, and Halina Zaleski for their substantial contributions throughout the project. NR 102 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD OCT PY 2016 VL 23 IS 20 BP 20257 EP 20268 DI 10.1007/s11356-016-7194-z PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA EB7VM UT WOS:000387599100019 PM 27447471 ER PT J AU Klick, J Yang, WQ Lee, JC Bruck, DJ AF Klick, Jimmy Yang, Wei Q. Lee, Jana C. Bruck, Denny J. TI Reduced spray programs for Drosophila suzukii management in berry crops SO INTERNATIONAL JOURNAL OF PEST MANAGEMENT LA English DT Article DE Border spray; alternate row middle spray; blueberry; raspberry; natural enemies; fruit knockdown ID SPOTTED-WING DROSOPHILA; COMMERCIAL APPLE ORCHARDS; DIPTERA DROSOPHILIDAE; PLUM CURCULIO; WEEVIL COLEOPTERA; PEST-MANAGEMENT; BORDER SPRAYS; FLIES DIPTERA; CODLING MOTH; INSECTICIDES AB Since the arrival of Drosophila suzukii M. (Diptera: Drosophilidae), field applications of broad-spectrum insecticides have increased in berry crop production to protect susceptible fruits from infestation. Field studies were conducted from 2011 to 2013 to determine whether alternate row middle or border spray programs could manage D. suzukii as well as complete sprays and have less of an impact on non-target arthropods. Four raspberry sites and one blueberry site were alternate row middle and border sprayed, respectively. Adult and larva counts of D. suzukii were compared to conventional complete spray programs. Non-target arthropods were evaluated 7 d post-harvest. In both reduced spray trials, no differences in mean adult numbers and larvae of D. suzukii were detected between treatments. Mean counts immediately after sprays (i.e. 3-12 d after treatment) were also similar in complete and alternate row sprays. Both reduced spray strategies had significantly more Stethorus spp.; additionally, alternate row sprays had significantly more Psyllobora spp. These reduced pesticide strategies are additional tools to consider in D. suzukii IPM programs that can reduce the area sprayed, application time, and input costs while conserving natural enemies. C1 [Klick, Jimmy] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yang, Wei Q.] Oregon State Univ, Dept Hort, North Willamette Res & Extens Ctr, Aurora, OR USA. [Lee, Jana C.; Bruck, Denny J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. [Klick, Jimmy] Driscoll Strawberry Associates, Oxnard, CA 93033 USA. [Bruck, Denny J.] DuPont Pioneer, Johnston, IA USA. RP Klick, J (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA.; Klick, J (reprint author), Driscoll Strawberry Associates, Oxnard, CA 93033 USA. EM jimmy.klick@driscolls.com FU Oregon State University Agricultural Research Foundation [ARF 4748A]; Oregon Blueberry Commission; WSARE [GW13-014]; USDA SCRI [2010-51181-21167]; USDA CRIS [5358-22000-037-00D] FX Funding was provided by Oregon State University Agricultural Research Foundation [grant number ARF 4748A]; Oregon Blueberry Commission; WSARE [grant number GW13-014]; USDA SCRI [grant number 2010-51181-21167]; and USDA CRIS [grant number 5358-22000-037-00D]. NR 45 TC 0 Z9 0 U1 15 U2 15 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0967-0874 EI 1366-5863 J9 INT J PEST MANAGE JI Int. J. Pest Manage. PD OCT-DEC PY 2016 VL 62 IS 4 BP 368 EP 377 DI 10.1080/09670874.2016.1222105 PG 10 WC Entomology SC Entomology GA DZ3WY UT WOS:000385781500014 ER PT J AU Li, F Zhuang, H Qiao, WW Zhang, JH Wang, YL AF Li, Feng Zhuang, Hong Qiao, Weiwei Zhang, Jianhao Wang, Yongli TI Effect of partial substitution of NaCl by KCl on physicochemical properties, biogenic amines and N-nitrosamines during ripening and storage of dry-cured bacon SO JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE LA English DT Article DE Dry-cured bacon; Sodium chloride; Potassium chloride; Physicochemical property; Biogenic amines; Nitrosodimethylamine ID PARTIAL SODIUM REPLACEMENT; MEAT-PRODUCTS; FERMENTED SAUSAGES; SENSORY PROPERTIES; POTASSIUM; CHLORIDE; SALTS; MICROBIOLOGY; PROTEOLYSIS; MAGNESIUM AB Effects of three salting treatments (Formulation II: 80 % NaCl + 20 % KCl; Formulation III: 60 % NaCl + 40 % KCl and Formulation IV: 40 % NaCl + 60 % KCl) on physicochemical properties, residual nitrite, N-nitrosamines and biogenic amines were compared with those of control bacons (Formulation I: 100 % NaCl) during processing and storage. Results showed that there were no significant differences among treatments for moisture, pH, and total volatile basic nitrogen (TVBN) content in dry-cured bacons during processing. The replacement of 40 % or less NaCl by KCl had no negative effects on the sensory quality of bacons during processing. Formulation III significantly reduced putrescine, cadaverine and histamine contents and enhanced nitrite residues compared with the control. After 12-day ripening and during storage, the substitution of NaCl by 60 % KCl significantly increased the N-nitrosodimethylamine (NDMA) content than the control. Principal component analysis showed that there were positive correlations between TVBN, biogenic amines (putrescine, cadaverine, histamine and tyramine) and NDMA, and negative correlation between NDMA and nitrite. These findings suggested the partial substitution of NaCl by KCl could be utilized for producing reduced-sodium dry-cured bacons to improve safety of finished products. C1 [Li, Feng; Wang, Yongli] Shandong Agr Univ, Coll Food Sci & Engn, 61 Daizong St, Tai An 271018, Shandong, Peoples R China. [Zhuang, Hong] ARS, Qual & Safety Assessment Res Unit, USDA, Athens, GA 30605 USA. [Qiao, Weiwei; Zhang, Jianhao; Wang, Yongli] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. RP Wang, YL (reprint author), Shandong Agr Univ, Coll Food Sci & Engn, 61 Daizong St, Tai An 271018, Shandong, Peoples R China.; Wang, YL (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang Rd, Nanjing 210095, Jiangsu, Peoples R China. EM wylily@sdau.edu.cn FU National Key Technology R&D Program in the 12th Five Year Plan of China [2012BAD28B01]; Science and Technology Foundation in Higher Education of Shandong Province [J16LE18] FX This study was supported by the National Key Technology R&D Program in the 12th Five Year Plan of China (2012BAD28B01) and Science and Technology Foundation in Higher Education of Shandong Province (J16LE18). NR 36 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0022-1155 EI 0975-8402 J9 J FOOD SCI TECH MYS JI J. Food Sci. Technol.-Mysore PD OCT PY 2016 VL 53 IS 10 BP 3795 EP 3805 DI 10.1007/s13197-016-2366-x PG 11 WC Food Science & Technology SC Food Science & Technology GA EB8TB UT WOS:000387662800018 PM 28017995 ER PT J AU Lu, AS Baranowski, T Hong, SL Buday, R Thompson, D Beltran, A Dadabhoy, HR Chen, TA AF Lu, Amy Shirong Baranowski, Tom Hong, S. Lee Buday, Richard Thompson, Debbe Beltran, Alicia Dadabhoy, Hafza Razak Chen, Tzu-An TI The Narrative Impact of Active Video Games on Physical Activity Among Children: A Feasibility Study SO JOURNAL OF MEDICAL INTERNET RESEARCH LA English DT Article DE video games; narration; behavior; child health; child obesity; motivation ID CHILDHOOD OBESITY PREVENTION; ENERGY-EXPENDITURE; BODY-COMPOSITION; HEALTH; INTERVENTIONS; ADOLESCENTS; PREVALENCE; MULTIMEDIA; ADULTHOOD; INSULIN AB Background: Active video games (AVGs) capable of inducing physical activity offer an innovative approach to combating childhood obesity. Unfortunately, children's AVG game play decreases quickly, underscoring the need to identify novel methods for player engagement. Narratives have been demonstrated to influence behaviors. Objective: The objective of this study was to test the hypothesis that a narrative would motivate increased AVG play, though a feasibility study that investigated the motivational effect of adding a previously developed narrative cutscene to an originally nonnarrative AVG, Nintendo Wii Sports Resort: Swordplay Showdown. Methods: A total of 40 overweight and obese 8-to 11-year-olds equally divided by sex played the AVG. Half (n=20) were randomly assigned to a narrative group that watched the narrative cutscene before game play. The other half played the game without watching it. Results: Children in the narrative group had significantly (P<.05) more steps per 10-second period (mean 3.2, SD 0.7) and overall (mean 523, SD 203) during game play compared with the nonnarrative group (10-second period: mean 2.7, SD 0.7; overall: mean 366, SD 172). Conclusions: The AVG with narrative induced increased physical activity. Additional research is needed to understand the mechanisms through which narrative increases physical activity during AVG game play. C1 [Lu, Amy Shirong] Northeastern Univ, Dept Commun Studies, Coll Arts Media & Design, 360 Huntington Ave,204 Lake Hall, Boston, MA 02115 USA. [Lu, Amy Shirong] Northeastern Univ, Dept Hlth Sci, Bouve Coll Hlth Sci, Boston, MA 02115 USA. [Baranowski, Tom; Thompson, Debbe; Beltran, Alicia; Dadabhoy, Hafza Razak] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Hong, S. Lee] Informat Control Co, Columbus, OH USA. [Buday, Richard] Archimage Inc, Houston, TX USA. [Chen, Tzu-An] Univ Texas Hlth Sci Ctr Houston, McGovern Med Sch, Houston, TX 77030 USA. RP Lu, AS (reprint author), Northeastern Univ, Dept Commun Studies, Coll Arts Media & Design, 360 Huntington Ave,204 Lake Hall, Boston, MA 02115 USA. EM a.lu@northeastern.edu OI Baranowski, Tom/0000-0002-0653-2222; Lu, Amy Shirong/0000-0002-8230-9049 FU National Cancer Institute [1R21CA158917-01A1]; US Department of Agriculture's Agricultural Research Service [58-6250-0-008] FX Leia Butler, Leopoldo Castillo, Robert Dolezal, Sakina Kazmi, and Kimberlyn Shirrels helped with data collection in the narrative development stage. This project was funded by a grant from the National Cancer Institute (1R21CA158917-01A1), "The Narrative Impact of Active Video Games on Physical Activity," and federal funds from the US Department of Agriculture's Agricultural Research Service under Cooperative Agreement No. 58-6250-0-008. NR 66 TC 0 Z9 0 U1 14 U2 14 PU JMIR PUBLICATIONS, INC PI TORONTO PA 59 WINNERS CIRCLE, TORONTO, ON M4L 3Y7, CANADA SN 1438-8871 J9 J MED INTERNET RES JI J. Med. Internet Res. PD OCT PY 2016 VL 18 IS 10 BP 101 EP 110 AR e272 DI 10.2196/jmir.6538 PG 10 WC Health Care Sciences & Services; Medical Informatics SC Health Care Sciences & Services; Medical Informatics GA EC2WT UT WOS:000387985700008 PM 27742605 ER PT J AU Roemmich, JN AF Roemmich, James N. TI Height-Adjustable Desks: Energy Expenditure, Liking, and Preference of Sitting and Standing SO JOURNAL OF PHYSICAL ACTIVITY & HEALTH LA English DT Article DE posture; word processing; workplace; workstation; liking ID PHYSICAL-ACTIVITY; NONEXERCISE ACTIVITY; METABOLIC SYNDROME; OBESITY; WORK; EPIDEMIC; BEHAVIOR; TIME AB Background: Breaking periods of sitting with standing may prevent chronic diseases and increase energy expenditure (EE). Sit-to-stand height adjustable desks may promote workplace standing, but workers have to be willing to stand for portions of the workday. Methods: For studies 1 and 2, EE was measured during word processing while sitting in a chair and while standing. Subjects scored their liking of each posture and time they would be willing to work in each posture during an 8-hour workday. Study 2 included an intervention of replacing subjects' sitting desks with a height adjustable desk. Liking of and willingness to work in each posture were measured before and after the 12-month intervention. Results: EE was 7.5 kcal/h greater when standing than when sitting. Subjects liked sitting more than standing in study 1. In study 2, liking of postures did not differ or change across 12 months use of height adjustable desks. Perceived willingness to stand decreased from 4.5 h/d at baseline to 3.4 h/d after 12 months. Conclusions: Standing rather than sitting increased EE by 7.5 kcal/h. Use of a height adjustable desk for 12 months did not alter the hedonic value of standing or sitting, which is promising for long-term increases in standing. C1 [Roemmich, James N.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Roemmich, JN (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM james.roemmich@ars.usda.gov FU USDA/Agricultural Research Service [USDA 5450-51000-049-00D, USDA 5450-51000-051-00D] FX The author thanks Joley Beeler, BS, for assistance with writing of the manuscript. Parts of this research were supported by the USDA/Agricultural Research Service, USDA 5450-51000-049-00D and USDA 5450-51000-051-00D. The contents of this publication do not necessarily reflect the views or policies of the USDA or the Agricultural Research Service, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. No financial disclosures were reported by the authors of this paper. This study is registered at www.clinicaltrials.gov (NCT01787643). NR 29 TC 0 Z9 0 U1 5 U2 5 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1543-3080 EI 1543-5474 J9 J PHYS ACT HEALTH JI J. Phys. Act. Health PD OCT PY 2016 VL 13 IS 10 BP 1094 EP 1099 DI 10.1123/jpah.2015-0397 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EB8ID UT WOS:000387633800010 PM 27256708 ER PT J AU Yu, XQ Li, XR Guo, TT Zhu, CS Wu, YY Mitchell, SE Roozeboom, KL Wang, DH Wang, ML Pederson, GA Tesso, TT Schnable, PS Bernardo, R Yu, JM AF Yu, Xiaoqing Li, Xianran Guo, Tingting Zhu, Chengsong Wu, Yuye Mitchell, Sharon E. Roozeboom, Kraig L. Wang, Donghai Wang, Ming Li Pederson, Gary A. Tesso, Tesfaye T. Schnable, Patrick S. Bernardo, Rex Yu, Jianming TI Genomic prediction contributing to a promising global strategy to turbocharge gene banks SO NATURE PLANTS LA English DT Article ID QUANTITATIVE TRAITS; COMPLEX TRAITS; SELECTION; PRODUCTIVITY; ENVIRONMENT; REGRESSION; MARKERS AB The 7.4 million plant accessions in gene banks are largely underutilized due to various resource constraints, but current genomic and analytic technologies are enabling us to mine this natural heritage. Here we report a proof-of-concept study to integrate genomic prediction into a broad germplasm evaluation process. First, a set of 962 biomass sorghum accessions were chosen as a reference set by germplasm curators. With high throughput genotyping-by-sequencing (GBS), we genetically characterized this reference set with 340,496 single nucleotide polymorphisms (SNPs). A set of 299 accessions was selected as the training set to represent the overall diversity of the reference set, and we phenotypically characterized the training set for biomass yield and other related traits. Cross-validation with multiple analytical methods using the data of this training set indicated high prediction accuracy for biomass yield. Empirical experiments with a 200-accession validation set chosen from the reference set confirmed high prediction accuracy. The potential to apply the prediction model to broader genetic contexts was also examined with an independent population. Detailed analyses on prediction reliability provided new insights into strategy optimization. The success of this project illustrates that a global, cost-effective strategy may be designed to assess the vast amount of valuable germplasm archived in 1,750 gene banks. C1 [Yu, Xiaoqing; Li, Xianran; Guo, Tingting; Zhu, Chengsong; Schnable, Patrick S.; Yu, Jianming] Iowa State Univ, Ames, IA 50011 USA. [Wu, Yuye; Roozeboom, Kraig L.; Wang, Donghai; Tesso, Tesfaye T.] Kansas State Univ, Manhattan, KS 66506 USA. [Mitchell, Sharon E.] Cornell Univ, Ithaca, NY 14853 USA. [Wang, Ming Li; Pederson, Gary A.] ARS, USDA, Griffin, GA 30223 USA. [Bernardo, Rex] Univ Minnesota, St Paul, MN 55108 USA. RP Yu, JM (reprint author), Iowa State Univ, Ames, IA 50011 USA. EM jmyu@iastate.edu FU Agriculture and Food Research Initiative competitive grant from the USDA National Institute of Food and Agriculture [2011-03587]; National Science Foundation [IOS-1238142]; Kansas State University Center for Sorghum Improvement; Iowa State University Raymond F. Baker Center for Plant Breeding; Iowa State University Plant Science Institute FX This work was supported by the Agriculture and Food Research Initiative competitive grant (2011-03587) from the USDA National Institute of Food and Agriculture, by the National Science Foundation grant IOS-1238142, by the Kansas State University Center for Sorghum Improvement, by the Iowa State University Raymond F. Baker Center for Plant Breeding and by the Iowa State University Plant Science Institute. We appreciate K. Mayfield, L. Lambright and S. Staggenborg from Chromatin for conducting experiments at Lubbock, Texas. NR 50 TC 1 Z9 1 U1 13 U2 13 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD OCT PY 2016 VL 2 IS 10 AR 16150 DI 10.1038/NPLANTS.2016.150 PG 7 WC Plant Sciences SC Plant Sciences GA EB4AW UT WOS:000387312400003 PM 27694945 ER PT J AU Henry, TJ Howard, SZ AF Henry, Thomas J. Howard, Samuel Z. TI REVISION OF THE NEOTROPICAL PLANT BUG GENUS SINERVUS STAL (HETEROPTERA: MIRIDAE: BRYOCORINAE: ECCRITOTARSINI), WITH THE DESCRIPTION OF FOUR NEW SPECIES AND A CLOSELY RELATED NEW GENUS SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Insecta; Hemiptera; Miridae; Bryocorinae; new genus; new species; new combinations; Neotropics AB The eccritotarsine plant bug genus Sinervus Stal is revised and the species S. apicalis, n. sp. from Honduras and S. cunealis, n. sp. and S. egeri, n. sp. from Brazil are described, bringing the total species in the genus to 11. Sinervus and the nine previously known species are diagnosed and the three new species are described. The genus Sinervaspartus, n. gen. is described to accommodate Spartacus discovittatus Carvalho, new combination (as the type species), Sinervus espartacoides Carvalho and Gomes, new combination, Spartacus venezuelanus Carvalho, new combination, and Sinervaspartus marginalis, n. sp. from Peru. Color dorsal images for all species, male genitalia for the new species, selected SEM micrographs, and keys to help distinguish Sinervus and Sinervaspartus and all included species are provided. Relationships with the New World genus Spartacus Distant and certain Old World genera are discussed. C1 [Henry, Thomas J.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012, Washington, DC 20013 USA. [Howard, Samuel Z.] Smithsonian Inst, Natl Museum Nat Hist, Dept Entomol, Washington, DC 20013 USA. [Howard, Samuel Z.] 42 Castro St,Apt 5, San Francisco, CA 94114 USA. RP Henry, TJ (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012, Washington, DC 20013 USA. EM thomas.henry@ars.usda.gov NR 21 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 533 EP 554 DI 10.4289/0013-8797.118.4.533 PG 22 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500005 ER PT J AU Smith, DR Dolan, AC AF Smith, David R. Dolan, Amelia C. TI A NEW SPECIES OF HUCKLEBERRY SAWFLY OF THE FORMER TRIBE PRISTOLINI (HYMENOPTERA: TENTHREDINIDAE) FROM MONTANA SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Symphyta; Nematinae; Pristolini; Melastola; Pristola; fruit feeders AB Pristiphora fructicola Smith and Dolan, n. sp., is described from Montana. It would belong to the former genus Melastola Wong of the former tribe Pristolini, recently synonymized with Pristiphora Latreille. Adults were numerous around Vaccinium globulare Rydberg (Ericaceae), the presumed host, where larvae feed in the fruits. The species is distinguished from the other two species previously placed in Melastola, P. resinicolor (Marlatt) and P. ferruginosa (Wong) (n. comb.). Several new distribution records and host plant associations are given for the species of "Pristolini." C1 [Smith, David R.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Dolan, Amelia C.] Montana State Univ, Montana Entomol Collect, Marsh Labs, Room 50,POB 173145, Bozeman, MT 59717 USA. RP Smith, DR (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com; amelia.clare1229@gmail.com FU Montana Department of Agriculture's Specialty Crop Block Grant Program FX We thank Chris Grinter, Illinois Natural History Survey, Champaign, IL, for loan of the type slide of Melastola ferruginosa and Derek Sikes, UAM, for loan of specimens. Michael Ivie, Montana State University, assisted in the determination of this species and loaned specimens for study. Paloma Amaral assisted with field work. This work was conducted with funding to Michael Ivie from a grant by the Montana Department of Agriculture's Specialty Crop Block Grant Program titled "Huckleberry Challenges: Pollinator Mysteries, Pests and New Invasive Threats." Work in the Custer Gallatin National Forest was conducted with permission of Mary C. Erickson, Forest Supervisor, U. S. National Forest Service, Bozeman. This is a contribution of the Montana Agricultural Experiment Station. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 7 TC 0 Z9 0 U1 1 U2 1 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 594 EP 601 DI 10.4289/0013-8797.118.4.594 PG 8 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500010 ER PT J AU Smith, DR Strazanac, JS AF Smith, David R. Strazanac, John S. TI ANNOTATED LIST OF WEST VIRGINIA SAWFLIES (HYMENOPTERA: SYMPHYTA) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE species list; Xyelidae; Pamphiliidae; Pergidae; Argidae; Cimbicidae; Diprionidae; Tenthredinidae; Siricidae; Xiphydriidae; Orussidae; Cephidae ID EASTERN UNITED-STATES; QUERCUSCOCCINEAE DYAR HYMENOPTERA; NEMATUS PANZER HYMENOPTERA; NORTH-AMERICA; CENTRAL KENTUCKY; HOST PLANTS; TENTHREDINIDAE; GENUS; WASHINGTON; ARGIDAE AB Records for 281 species of sawflies in 11 families are listed for West Virginia. Results are based on extensive Malaise trap collections at several sites in Hardy, Tucker, and Pocohantas counties and data from museum specimens. County records and recorded host plants are given for each species. C1 [Smith, David R.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Strazanac, John S.] West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. RP Smith, DR (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com; fritzstraz@gmail.com NR 73 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 602 EP 616 DI 10.4289/0013-8797.118.4.602 PG 15 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500011 ER PT J AU Adaime, R de Jesus-Barros, CR Uramoto, K Norrbom, AL Zucchi, RA AF Adaime, Ricardo de Jesus-Barros, Cristiane Ramos Uramoto, Keiko Norrbom, Allen L. Zucchi, Roberto Antonio TI First Record of Anastrepha zacharyi Norrbom (Diptera, Tephritidae) in Brazil, and Notes on its Host Plant and Parasitoid SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material ID HYMENOPTERA; STATE C1 [Adaime, Ricardo; de Jesus-Barros, Cristiane Ramos] Embrapa Amapa, BR-68903419 Macapa, Amapa, Brazil. [Uramoto, Keiko; Zucchi, Roberto Antonio] Univ Sao Paulo, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, BR-13418900 Sao Paulo, Brazil. [Norrbom, Allen L.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. RP Adaime, R (reprint author), Embrapa Amapa, BR-68903419 Macapa, Amapa, Brazil. EM ricardo.adaime@embrapa.br NR 15 TC 0 Z9 0 U1 2 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD OCT PY 2016 VL 118 IS 4 BP 636 EP 640 DI 10.4289/0013-8797.118.4.636 PG 5 WC Entomology SC Entomology GA EB9SX UT WOS:000387738500014 ER PT J AU Karlen, SD Zhang, CC Peck, ML Smith, RA Padmakshan, D Helmich, KE Free, HCA Lee, S Smith, BG Lu, FC Sedbrook, JC Sibout, R Grabber, JH Runge, TM Mysore, KS Harris, PJ Bartley, LE Ralph, J AF Karlen, Steven D. Zhang, Chengcheng Peck, Matthew L. Smith, Rebecca A. Padmakshan, Dharshana Helmich, Kate E. Free, Heather C. A. Lee, Seonghee Smith, Bronwen G. Lu, Fachuang Sedbrook, John C. Sibout, Richard Grabber, John H. Runge, Troy M. Mysore, Kirankumar S. Harris, Philip J. Bartley, Laura E. Ralph, John TI Monolignol ferulate conjugates are naturally incorporated into plant lignins SO SCIENCE ADVANCES LA English DT Article ID CELL-WALLS; STRUCTURAL-CHARACTERIZATION; TRANSFERASE PMT; DFRC METHOD; RICE; LIGNIFICATION; OVEREXPRESSION; IDENTIFICATION; BIOSYNTHESIS; PERSPECTIVE AB Angiosperms represent most of the terrestrial plants and are the primary research focus for the conversion of biomass to liquid fuels and coproducts. Lignin limits our access to fibers and represents a large fraction of the chemical energy stored in plant cell walls. Recently, the incorporation of monolignol ferulates into lignin polymers was accomplished via the engineering of an exotic transferase into commercially relevant poplar. We report that various angiosperm species might have convergently evolved to natively produce lignins that incorporate monolignol ferulate conjugates. We show that this activity may be accomplished by a BAHD feruloyl-coenzyme A monolignol transferase, OsFMT1 (AT5), in rice and its orthologs in other monocots. C1 [Karlen, Steven D.; Smith, Rebecca A.; Padmakshan, Dharshana; Helmich, Kate E.; Harris, Philip J.; Ralph, John] Univ Wisconsin, Wisconsin Energy Inst, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA. [Karlen, Steven D.; Smith, Rebecca A.; Helmich, Kate E.; Lu, Fachuang; Ralph, John] Univ Wisconsin, Dept Bio Chem, Madison, WI 53706 USA. [Zhang, Chengcheng; Peck, Matthew L.; Bartley, Laura E.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Free, Heather C. A.; Harris, Philip J.] Univ Auckland, Sch Biol Sci, Auckland, New Zealand. [Free, Heather C. A.; Smith, Bronwen G.] Univ Auckland, Sch Chem Sci, Auckland, New Zealand. [Lee, Seonghee] Univ Florida, IFAS, Dept Hort Sci, Gulf Coast Res & Educ Ctr, 14625 Cty Rd 672, Wimauma, FL 33598 USA. [Grabber, John H.] Illinois State Univ, Sch Biol Sci, Dept Energy Great Lakes Bioenergy Res Ctr, Normal, IL 61790 USA. [Smith, Bronwen G.; Sedbrook, John C.; Grabber, John H.] AgroParisTech, Inst Jean Pierre Bourgin, UMR 1318, Saclay Plant Sci, F-78000 Versailles, France. [Peck, Matthew L.; Free, Heather C. A.; Sibout, Richard; Grabber, John H.] Inst Natl Rech Agronom, F-78000 Versailles, France. [Helmich, Kate E.; Free, Heather C. A.; Lu, Fachuang; Grabber, John H.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Free, Heather C. A.; Sibout, Richard] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Smith, Rebecca A.; Lee, Seonghee] Samuel Roberts Noble Fdn Inc, Plant Biol Div, Ardmore, OK 73401 USA. RP Ralph, J (reprint author), Univ Wisconsin, Wisconsin Energy Inst, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA.; Ralph, J (reprint author), Univ Wisconsin, Dept Bio Chem, Madison, WI 53706 USA.; Bartley, LE (reprint author), Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. EM lbartley@ou.edu; jralph@wisc.edu NR 43 TC 1 Z9 1 U1 6 U2 6 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 2375-2548 J9 SCI ADV JI Sci. Adv. PD OCT PY 2016 VL 2 IS 10 AR e1600393 DI 10.1126/sciadv.1600393 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC2YV UT WOS:000387991500008 ER PT J AU Shi, GJ Zhang, ZC Friesen, TL Raats, D Fahima, T Brueggeman, RS Lu, SW Trick, HN Liu, ZH Chao, W Frenkel, Z Xu, SS Rasmussen, JB Faris, JD AF Shi, Gongjun Zhang, Zengcui Friesen, Timothy L. Raats, Dina Fahima, Tzion Brueggeman, Robert S. Lu, Shunwen Trick, Harold N. Liu, Zhaohui Chao, Wun Frenkel, Zeev Xu, Steven S. Rasmussen, Jack B. Faris, Justin D. TI The hijacking of a receptor kinase-driven pathway by a wheat fungal pathogen leads to disease SO SCIENCE ADVANCES LA English DT Article ID PYRENOPHORA-TRITICI-REPENTIS; RESISTANCE GENE; PROTEIN; SYSTEM; TOXA; SUSCEPTIBILITY; INFECTION; EFFECTOR; REVEALS; PLANTS AB Necrotrophic pathogens live and feed on dying tissue, but their interactions with plants are not well understood compared to biotrophic pathogens. The wheat Snn1 gene confers susceptibility to strains of the necrotrophic pathogen Parastagonospora nodorum that produce the SnTox1 protein. We report the positional cloning of Snn1, a member of the wall-associated kinase class of receptors, which are known to drive pathways for biotrophic pathogen resistance. Recognition of SnTox1 by Snn1 activates programmed cell death, which allows this necrotroph to gain nutrients and sporulate. These results demonstrate that necrotrophic pathogens such as P. nodorum hijack host molecular pathways that are typically involved in resistance to biotrophic pathogens, revealing the complex nature of susceptibility and resistance in necrotrophic and biotrophic pathogen interactions with plants. C1 [Shi, Gongjun; Friesen, Timothy L.; Brueggeman, Robert S.; Liu, Zhaohui; Rasmussen, Jack B.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Zhang, Zengcui; Friesen, Timothy L.; Lu, Shunwen; Xu, Steven S.; Faris, Justin D.] ARS, USDA, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. [Raats, Dina; Fahima, Tzion; Frenkel, Zeev] Univ Haifa, Inst Evolut, IL-3498838 Haifa, Israel. [Raats, Dina; Fahima, Tzion; Frenkel, Zeev] Univ Haifa, Dept Evolutionary & Environm Biol, IL-3498838 Haifa, Israel. [Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Chao, Wun] ARS, USDA, Sunflower & Plant Biol Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. RP Faris, JD (reprint author), ARS, USDA, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. EM justin.faris@ars.usda.gov NR 36 TC 2 Z9 2 U1 5 U2 5 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 2375-2548 J9 SCI ADV JI Sci. Adv. PD OCT PY 2016 VL 2 IS 10 AR e1600822 DI 10.1126/sciadv.1600822 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EC2YV UT WOS:000387991500018 PM 27819043 ER PT J AU Berstis, L Elder, T Crowley, M Beckham, GT AF Berstis, Laura Elder, Thomas Crowley, Michael Beckham, Gregg T. TI Radical Nature of C-Lignin SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Lignin biosynthesis; Lignin valorization; Caffeoyl alcohol; Caffeyl alcohol; Bond dissociation enthalpy ID BOND-DISSOCIATION ENTHALPIES; MODEL COMPOUNDS; LIGNOCELLULOSIC BIOMASS; BETA-GUAIACYL; NONCOVALENT INTERACTIONS; FUNCTIONAL MATERIALS; PHYSICAL-PROPERTIES; CRYSTAL-STRUCTURE; KRAFT LIGNIN; BIOSYNTHESIS AB The recently discovered lignin composed of caffeoyl alcohol monolignols or C-lignin is particularly intriguing given its homogeneous, linear polymeric structure and exclusive benzodioxane linkage between monomers. By virtue of this simplified chemistry, the potential emerges for improved valorization strategies with C-lignin relative to other natural heterogeneous lignins. To better understand caffeoyl alcohol polymers, we characterize the thermodynamics of the radical recombination dimerization reactions forming the benzodioxane linkage and the bond dissociation into radical monolignol products. These properties are also predicted for the cross-coupling of caffeoyl alcohol with the natural monolignols, coniferyl alcohol, sinapyl alcohol, and p-coumaryl alcohol, in anticipation of polymers potentially enabled by genetic modification. The average BDEs for the C-lignin benzodioxane alpha- and beta-bonds are 56.5 and 63.4 kcal/mol, respectively, with similar enthalpies for heterodimers. The BDE of the alpha-bond within the benzodioxane linkage is consistently greater than that of the beta-bond in all dimers of each stereochemical arrangement, explained by the ability the alpha-carbon radical generated to delocalize onto the adjacent phenyl ring. Relative thermodynamics of the heterodimers demonstrates that the substituents on the phenyl ring directly neighboring the bond coupling the monolignols more strongly impact the dimer bond strengths and product stability, compared to the substituents present on the terminal phenyl ring. Enthalpy comparisons furthermore demonstrate that the erythro stereochemical configurations of the benzodioxane bond are slightly less thermodynamically stable than the threo configurations. The overall differences in strength of bonds and reaction enthalpies between stereoisomers are generally found to be insignificant, supporting that postcoupling rearomatization is under kinetic control. Projecting the lowest-energy stereoisomer internal coordinates to longer polymer C-lignin strands highlights how significantly the stereochemical outcomes in polymerization may impact the macromolecular structure and in turn material and chemical properties. Through these comparisons of geometry, bond strengths, and reaction enthalpies, we shed light on the distinctive properties of C-lignin's radical recombination and decomposition chemistry, and its potential as a natural lignin solution for biorefinery feedstocks and unique materials science applications. C1 [Berstis, Laura; Crowley, Michael; Beckham, Gregg T.] Natl Bioenergy Ctr, Natl Renewable Energy Lab, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Elder, Thomas] USDA, Forest Serv, Southern Res Stn, 521 Devall Dr, Auburn, AL 36849 USA. RP Crowley, M; Beckham, GT (reprint author), Natl Bioenergy Ctr, Natl Renewable Energy Lab, 15013 Denver West Pkwy, Golden, CO 80401 USA. EM Michael.Crowley@nrel.gov; Gregg.Beckham@nrel.gov FU US Department of Energy Bioenergy Technologies Office; DOE Office of EERE [DE-AC36-08GO28308] FX This work was supported by the US Department of Energy Bioenergy Technologies Office. We are grateful for supercomputer time on Stampede provided by the Texas Advanced Computing Center (TACC) at the University of Texas at Austin through MCB-09159 and the NREL Computational Sciences Center, which is supported by the DOE Office of EERE under Contract No. DE-AC36-08GO28308. NR 63 TC 0 Z9 0 U1 5 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD OCT PY 2016 VL 4 IS 10 BP 5327 EP 5335 DI 10.1021/acssuschemeng.6b00520 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DY0MS UT WOS:000384791500024 ER PT J AU Abernethy, EF Turner, KL Beasley, JC DeVault, TL Pitt, WC Rhodes, OE AF Abernethy, Erin F. Turner, Kelsey L. Beasley, James C. DeVault, Travis L. Pitt, William C. Rhodes, Olin E., Jr. TI Carcasses of invasive species are predominantly utilized by invasive scavengers in an island ecosystem SO ECOSPHERE LA English DT Article DE cane toads; cannibalism; carrion; ecosystem function; Hawai'i; invasive species; mongoose; scavenging ID UNGULATE CARCASSES; AVIAN SCAVENGERS; CARRION; DECOMPOSITION; IMPACTS; SUCCESSION; PREDATORS; AUSTRALIA; NITROGEN; BIOLOGY AB Invasive species have significantly affected ecosystems, particularly islands, and species invasions continue with increasing globalization. Largely unstudied, the influence of invasive species on island ecosystem functions, especially scavenging and decomposition, could be substantive. Quantifying carcass utilization by different scavengers and shifts in community dynamics in the presence of invasive animals is of particular interest for understanding impacts on nutrient recycling. Invasive species could benefit greatly from carcass resources within highly invaded island ecosystems, through increased invasion success and population growth, subsequently exacerbating their impacts on native species. We quantified how experimentally placed invasive amphibian, reptile, small mammal, and bird carcasses were utilized by vertebrate and invertebrate scavengers on the Big Island of Hawai'i in three island habitats: a barren lava field, a vegetated lava field, and a rainforest. We used camera traps to record vertebrate scavengers removing carcasses and elapsed time until removal. We evaluated differences in scavenging between vertebrates and invertebrates and within the vertebrate community across different habitats and carcass types. Despite the small carcass sizes (<1 kg) used in this study, 55% of carcasses were removed by vertebrate scavengers, all invasive: mongoose, rodents, cats, pigs, and a common myna. Our data indicate that invasive vertebrate scavengers in this island ecosystem are highly efficient at assimilating a range of carrion resources across a variety of habitats. Carcasses of invasive animals could contribute substantially to energy budgets of other invasive vertebrate species. This may be a critical component contributing to successful invasions especially on islands and subsequent impacts on ecosystem function. C1 [Abernethy, Erin F.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Abernethy, Erin F.; Turner, Kelsey L.; Beasley, James C.; Rhodes, Olin E., Jr.] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA. [Turner, Kelsey L.; Beasley, James C.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [DeVault, Travis L.] USDA APHIS, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Pitt, William C.] USDA APHIS, Natl Wildlife Res Ctr, Hilo, HI 96720 USA. [Abernethy, Erin F.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Pitt, William C.] Natl Zool Pk, Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. RP Abernethy, EF (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA.; Abernethy, EF (reprint author), Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA.; Abernethy, EF (reprint author), Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. EM efabernethy@gmail.com FU University of Georgia Research Foundation; USDA NWRC Hilo Field Station [12-7415-0936-CA]; US Department of Energy [DE-FC09-07SR22506]; agency of the US Government FX We appreciate the field assistance of Shem Unger. We sincerely thank Kelton Kotake, Bob Sugihara, Dean Foster, Tom McAuliffe, and Aaron Shiels at the USDA National Wildlife Research Center Hilo Field Station for making this study possible. We thank Hawai'i Volcanoes National Park and the Natural Area Reserve System of HI DLNR for giving us a location and permit to conduct our study. This work was supported through Cooperative Agreements among the University of Georgia Research Foundation, the USDA NWRC Hilo Field Station (No. 12-7415-0936-CA), and the US Department of Energy (No. DE-FC09-07SR22506). This paper was prepared as an account of work sponsored by an agency of the US Government. Neither the US Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the US Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the US Government or any agency thereof. NR 61 TC 0 Z9 0 U1 15 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01496 DI 10.1002/ecs2.1496 PG 15 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300034 ER PT J AU Conner, MM Keane, JJ Gallagher, CV Munton, TE Shaklee, PA AF Conner, Mary M. Keane, John J. Gallagher, Claire V. Munton, Thomas E. Shaklee, Paula A. TI Comparing estimates of population change from occupancy and mark-recapture models for a territorial species SO ECOSPHERE LA English DT Article DE Bayesian MCMC approach; California spotted owl; mark-resight; monitoring; occupancy model; Pradel's temporal symmetry model; realized population change; Sierra Nevada; Strix occidentalis occidentalis ID ESTIMATING SITE OCCUPANCY; CALIFORNIA SPOTTED OWL; ESTIMATING ABUNDANCE; BAYESIAN-APPROACH; LOCAL EXTINCTION; GROWTH RATE; DYNAMICS; TRENDS; COLONIZATION; INFERENCE AB Monitoring studies often use marked animals to estimate population abundance at small spatial scales. However, at smaller scales, occupancy sampling, which uses detection/nondetection data, may be useful where sites are approximately territories, and occupancy dynamics should be strongly correlated with population dynamics. Occupancy monitoring has advantages in that it is less expensive and invasive, and marked animals are not needed. Here, we used empirical data to determine whether and when change in occupancy is a good proxy for population change for a territorial species. As part of this overall goal, we also compared maximum-likelihood estimates using a model-averaging approach with a Bayesian MCMC approach. We used field data collected from 1993 to 2013 on three study areas for California spotted owls (Strix occidentalis occidentalis), a territorial species. Although correlations for trajectories of realized population change (Delta(t)) between territory occupancy and Pradel models were moderate to high for Bayesian MCMC-based estimates and high for model-averaged estimates, magnitudes of the trajectories were different with the Pradel model reporting greater magnitudes of change. For the two areas showing a decline, Delta(t) for the Pradel model was approximately 20-30% lower than for the occupancy model, and 25% higher in the area showing an increase. These differences can arise because the occupancy model is less sensitive, in that if two owls share a territory, the loss of one may be reflected in survival and, consequently in Delta(t) by the Pradel model, but because the territory remains occupied it is not reflected by the occupancy model. Bayesian MCMC-based and model-averaged estimates of Delta(t) were in close agreement in pattern (correlation >= 0.74) and magnitude (relative differences of last Delta(t) were <= 5%) for both occupancy and mark-resight models. Results from the Pradel model may lead to conservation actions necessary to avoid high extinction or extirpation risk for small populations, while results from the territory occupancy model may result in status quo management. We found both Bayesian MCMC-based and model-averaged estimates of Delta(t) robust approaches to evaluate population trends. However, we recommend the Bayesian MCMC approach for estimating risk (e.g., probability of declines) for retrospective analyses. C1 [Conner, Mary M.; Keane, John J.; Gallagher, Claire V.; Shaklee, Paula A.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. [Munton, Thomas E.] US Forest Serv, USDA, Pacific Southwest Res Stn, 2081 East Sierra Ave, Fresno, CA 93710 USA. [Conner, Mary M.] Utah State Univ, Dept Wildland Resources, 5320 Old Main Hill, Logan, UT 84322 USA. RP Conner, MM (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA.; Conner, MM (reprint author), Utah State Univ, Dept Wildland Resources, 5320 Old Main Hill, Logan, UT 84322 USA. EM mary.conner@usu.edu FU U.S. Forest Service Region 5, the Pacific Southwest Research Station FX We thank the many field technicians who carefully collected data over the 20 yr of the study. In particular, we thank those who ran field projects were involved for the long haul (> 7 yr), or helped with data analyses including George Steger, Jennifer Blakesley, Dan Shaw, and Rick Scherer, Susan Sutton-Mazzocco, Lori Werner, Mason Werner, Kevin Mazzocco, Kenneth Johnson, Rachel Kussow, M. Warner, and Alfred Peet for their efforts in the field and out, which resulted in such a high-quality and long-term data set. We thank Michael Ebinger for providing invaluable help with the MATLAB code we used to generate our encounter histories. We thank Angela White, Seth Bigelow, Matthew Bokach, Ken Hubbert, and Tray Biasiolli, for helpful comments on drafts of the manuscript. Finally, we thank Brian Halstead for an exceptionally insightful review. Funding for our analysis was provided primarily for U.S. Forest Service Region 5, the Pacific Southwest Research Station. NR 60 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01538 DI 10.1002/ecs2.1538 PG 20 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300062 ER PT J AU Endress, BA Naylor, BJ Pekin, BK Wisdom, MJ AF Endress, Bryan A. Naylor, Bridgett J. Pekin, Burak K. Wisdom, Michael J. TI Aboveground and belowground mammalian herbivores regulate the demography of deciduous woody species in conifer forests SO ECOSPHERE LA English DT Article DE cattle; elk; grazing; North America; Oregon; plant population dynamics; plant-herbivore interactions; Populus trichocarpa; Salix scouleriana; trophic cascades; ungulate herbivory ID UNGULATE HERBIVORY; PLANT-COMMUNITIES; PRIMARY PRODUCTIVITY; INSECT HERBIVORY; BOTTOM-UP; TOP-DOWN; RECRUITMENT; VEGETATION; ECOSYSTEMS; RESPONSES AB Mammalian herbivory can have profound impacts on plant population and community dynamics. However, our understanding of specific herbivore effects remains limited, even in regions with high densities of domestic and wild herbivores, such as the semiarid conifer forests of western North America. We conducted a seven-year manipulative experiment to evaluate the effects of herbivory by two common ungulates, Cervus elaphus Rocky Mountain elk) and cattle Bos taurus (domestic cattle) on growth and survival of two woody deciduous species, Populus trichocarpa (cottonwood) and Salix scouleriana (Scouler's willow) in postfire early-successional forest stands. Additionally, we monitored belowground herbivory by Thomomys talpoides (pocket gopher) and explored effects of both aboveground and belowground herbivory on plant vital rates. Three, approximately 7 ha exclosures were constructed, and each was divided into 1-ha plots. Seven herbivory treatments were then randomly assigned to the plots: three levels of herbivory (low, moderate, and high) for both cattle and elk, and one complete ungulate exclusion treatment. Treatments were implemented for seven years. Results showed that cattle and elk substantially reduced height and growth of both cottonwood and willow. Elk had a larger effect on growth and subsequent plant height than cattle, especially for cottonwood, and elk effects occurred even at low herbivore densities. Pocket gophers had a strong effect on survival of both plant species while herbivory by ungulates did not. However, we documented significant interaction effects of aboveground and belowground herbivory on survival. Our study is one of the first to evaluate top-down regulation by multiple herbivore species at varying densities. Results suggest that traditional exclosure studies that treat herbivory as a binary factor (either present or absent) may not be sufficient to characterize top-down regulation on plant demography. Rather, the strength of top-down regulation varies depending on a number of factors including herbivore species, herbivore density, interactions among multiple herbivore species, and varying tolerance levels of different plant species to herbivory. C1 [Endress, Bryan A.] Oregon State Univ, Dept Anim & Rangeland Sci, Eastern Oregon Agr & Nat Resource Program, La Grande, OR 97850 USA. [Naylor, Bridgett J.; Wisdom, Michael J.] US Forest Serv, USDA, Pacific Northwest Res Stn, La Grande, OR 97850 USA. [Pekin, Burak K.] Istanbul Tech Univ, Eurasia Inst Earth Sci, TR-34469 Istanbul, Turkey. RP Endress, BA (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Eastern Oregon Agr & Nat Resource Program, La Grande, OR 97850 USA. EM bryan.endress@oregonstate.edu FU USDA Forest Service Pacific Norwest Research Station, Blue Mountains Demonstration Project and Oregon State University FX We are grateful for the 12 summer technicians who helped collect field data for our research. We also thank Jennifer Hafer, Kent Coe, Brian Dick, Ryan Kennedy, and Dennis Rea for support on all aspects of the research and Tim DelCurto, Martin Vavra, Abe Clark, John Cook, and Rachael Cook for designing and implementing the cattle and elk herbivory treatments. Research was funded by the USDA Forest Service Pacific Norwest Research Station, Blue Mountains Demonstration Project and Oregon State University. Research was conducted under approval and guidance by an Institutional Animal Care and Use Committee (IACUC 92-F-0004), as required by the United States Animal Welfare Act of 1985 and its regulations. We followed protocols established specifically by the IACUC for conducting cattle and elk research in our study area (Wisdom et al. 1993). NR 71 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01530 DI 10.1002/ecs2.1530 PG 18 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300056 ER PT J AU Fay, PA Guntenspergen, GR Olker, JH Johnson, WC AF Fay, Philip A. Guntenspergen, Glenn R. Olker, Jennifer H. Johnson, W. Carter TI Climate change impacts on freshwater wetland hydrology and vegetation cover cycling along a regional aridity gradient SO ECOSPHERE LA English DT Article DE ecosystem models; ecosystem services; grasslands; Prairie Pothole Region; precipitation gradient; wetland complexes; wetland conservation; WETLANDSCAPE ID PRAIRIE POTHOLE REGION; DYNAMICS AB Global mean temperature may increase up to 6 degrees C by the end of this century and together with precipitation change may steepen regional aridity gradients. The hydrology, productivity, and ecosystem services from freshwater wetlands depend on their future water balance. We simulated the hydrology and vegetation dynamics of wetland complexes in the North American Prairie Pothole Region with the WETLANDSCAPE model. Simulations for 63 precipitation x temperature combinations spanning 6 degrees C warming and - 20% to + 20% annual precipitation change at 19 locations along a mid-continental aridity gradient showed that aridity explained up to 99% of the variation in wetland stage and hydroperiod for all wetland permanence types, and in vegetation cycling for semipermanent wetlands. The magnitude and direction of hydrologic responses depended on whether climate changes increased or decreased water deficits. Warming to 6 degrees C and 20% less precipitation increased wetland water deficits and more strongly decreased wetland stage and hydroperiod from historic levels at low aridity, especially in semipermanent wetlands, where peak vegetation cycling (Cover Cycle Index, CCI) also shifted to lower aridity. In contrast, 20% more precipitation decreased water deficits, increasing wetland stage and hydroperiod most strongly in shallow wetlands at high aridity, but filling semipermanent wetlands and reducing CCI at low aridity. All climate changes narrowed the range of aridity favorable to high productivity. Climate changes that reduce water deficits may help maintain wetlands at high aridity at the expense of those at low aridity, but with warming certain, increased deficits are more likely and will help maintain wetlands at lower aridity but exacerbate loss of wetlands at high aridity. Thus, there is likely not a universally applicable approach to mitigating climate change impacts on freshwater wetlands across regional aridity gradients. Conservation strategies need to account for aridity-specific effects of climate change on freshwater wetland ecosystems. C1 [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. [Guntenspergen, Glenn R.] Patuxent Wildlife Res Ctr, USGS, Laurel, MD 20708 USA. [Olker, Jennifer H.] Univ Minnesota Duluth, Nat Resources Res Inst, 5013 Miller Trunk Highway, Duluth, MN 55811 USA. [Johnson, W. Carter] South Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. RP Fay, PA (reprint author), USDA ARS, Grassland Soil & Water Res Lab, 808 E Blackland Rd, Temple, TX 76502 USA. EM philip.fay@ars.usda.gov FU National Science Foundation [1339944, 1340413]; U.S. Geological Survey Climate and Land-Use Research and Development Program; USDA-Agricultural Research Service Climate; Air and Soils National Program; USDA-NIFA [2010-12865615-20632] FX We thank Anne Gibson for generating the weather scenarios and acknowledge funding from the National Science Foundation (Behavioral and Cognitive Sciences 1339944, Emerging Frontiers 1340413), U.S. Geological Survey Climate and Land-Use Research and Development Program, USDA-Agricultural Research Service Climate, Air and Soils National Program, and USDA-NIFA (2010-12865615-20632). Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. USDA is an Equal Opportunity Employer. The authors declare no conflict of interests. NR 29 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01504 DI 10.1002/ecs2.1504 PG 12 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300042 ER PT J AU Monahan, WB Rosemartin, A Gerst, KL Fisichelli, NA Ault, T Schwartz, MD Gross, JE Weltzin, JF AF Monahan, William B. Rosemartin, Alyssa Gerst, Katharine L. Fisichelli, Nicholas A. Ault, Toby Schwartz, Mark D. Gross, John E. Weltzin, Jake F. TI Climate change is advancing spring onset across the US national park system SO ECOSPHERE LA English DT Article DE climate change; landscape context; monitoring; national parks; phenology; protected areas; Special Feature: Science for Our National Parks' Second Century; spring index; United States ID UNITED-STATES; PHENOLOGICAL RESPONSE; FLOWERING PHENOLOGY; PLANT PHENOLOGY; MANAGEMENT; INDEXES; DESERT; TRENDS; FROST AB Many U.S. national parks are already at the extreme warm end of their historical temperature distributions. With rapidly warming conditions, park resource management will be enhanced by information on seasonality of climate that supports adjustments in the timing of activities such as treating invasive species, operating visitor facilities, and scheduling climate-related events (e.g., flower festivals and fall leaf-viewing). Seasonal changes in vegetation, such as pollen, seed, and fruit production, are important drivers of ecological processes in parks, and phenology has thus been identified as a key indicator for park monitoring. Phenology is also one of the most proximate biological responses to climate change. Here, we use estimates of start of spring based on climatically modeled dates of first leaf and first bloom derived from indicator plant species to evaluate the recent timing of spring onset (past 10-30 yr) in each U.S. natural resource park relative to its historical range of variability across the past 112 yr (1901-2012). Of the 276 high latitude to subtropical parks examined, spring is advancing in approximately three-quarters of parks (76%), and 53% of parks are experiencing "extreme" early springs that exceed 95% of historical conditions. Our results demonstrate how changes in climate seasonality are important for understanding ecological responses to climate change, and further how spatial variability in effects of climate change necessitates different approaches to management. We discuss how our results inform climate change adaptation challenges and opportunities facing parks, with implications for other protected areas, by exploring consequences for resource management and planning. C1 [Monahan, William B.] Natl Pk Serv, Inventory & Monitoring Div, Nat Resource Stewardship & Sci, 1201 Oakridge Dr, Ft Collins, CO 80525 USA. [Monahan, William B.] Forest Serv, Forest Hlth Technol Enterprise Team, USDA, 2150A Ctr Ave,Suite 331, Ft Collins, CO 80526 USA. [Rosemartin, Alyssa; Gerst, Katharine L.] USA Natl Phenol Network, Natl Coordinating Off, 1311 E 4th St, Tucson, AZ 85721 USA. [Rosemartin, Alyssa; Gerst, Katharine L.] Univ Arizona, Sch Nat Resources & Environm, 1311 E 4th St, Tucson, AZ 85721 USA. [Fisichelli, Nicholas A.; Gross, John E.] Natl Pk Serv, Climate Change Response Program, Nat Resource Stewardship & Sci, 1201 Oakridge Dr, Ft Collins, CO 80525 USA. [Fisichelli, Nicholas A.] Acad Natl Pk, Schood Inst, Forest Ecol Program, POB 277, Winter Harbor, ME 04693 USA. [Ault, Toby] Cornell Univ, Dept Earth & Atmospher Sci, 1113 Bradfield, Ithaca, NY 14853 USA. [Schwartz, Mark D.] Univ Wisconsin, Dept Geog, POB 413, Milwaukee, WI 53201 USA. [Weltzin, Jake F.] US Geol Survey, Tucson, AZ 85721 USA. RP Monahan, WB (reprint author), Natl Pk Serv, Inventory & Monitoring Div, Nat Resource Stewardship & Sci, 1201 Oakridge Dr, Ft Collins, CO 80525 USA.; Monahan, WB (reprint author), Forest Serv, Forest Hlth Technol Enterprise Team, USDA, 2150A Ctr Ave,Suite 331, Ft Collins, CO 80526 USA. EM wmonahan@fs.fed.us FU NPS landscape dynamics monitoring project, NPScape; NASA-NPS Landscape Climate Change Vulnerability Project (NASA Applied Sciences program) [10-BIOCLIM10-0034]; United States Geological Survey [G14AC00405] FX This work was supported by the NPS landscape dynamics monitoring project, NPScape, the NASA-NPS Landscape Climate Change Vulnerability Project (NASA Applied Sciences program award number 10-BIOCLIM10-0034), and by Cooperative Agreement (G14AC00405) from the United States Geological Survey to the University of Arizona. The SI-x models were developed using phenological data that are now available from the National Phenology Database at the USA National Phenology Network. We thank reviewers and colleagues who provided comments that greatly improved an earlier version of this manuscript: Timothy Assal, Jherime Kellermann, Abraham Miller-Rushing, Jeff Morisette, David Thoma, and John Paul Schmit. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. government. NR 88 TC 1 Z9 1 U1 28 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01465 DI 10.1002/ecs2.1465 PG 17 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300008 ER PT J AU Moran, MS Heilman, P Peters, DPC Collins, CH AF Moran, M. Susan Heilman, Philip Peters, Debra P. C. Collins, Chandra Holifield TI Agroecosystem research with big data and a modified scientific method using machine learning concepts SO ECOSPHERE LA English DT Article DE big data; experimental watersheds, ranges, and forests; Long-term Agro-ecosystem-Research (LTAR); long-term data; network science; regional-scale analyses ID EARLY 21ST-CENTURY; USE EFFICIENCY; PATTERNS; ECOLOGY; COLLECTION; DROUGHT; IMPACT; BIOMES AB Long-term studies of agroecosystems distributed across the North American continent are providing an extraordinary understanding of regional environmental dynamics. The new Long-Term Agro-ecosystem Research (LTAR) network (organized in 2012) has designed an explicit cross-site research program with multiple U.S. Department of Agriculture (USDA) experimental watersheds, ranges, and forests. Here, we report results from studies using a modified scientific method that includes learning through time that was implemented over the past five years with long-term data from USDA experimental sites in coordination with other networks. The results offer a compelling argument for the LTAR concept of combining site-based expertise with network-wide coordination and collaboration to arrive at more accurate scientific conclusions than possible from individual researchers working alone. Simply put, without site-based expertise and cross-site communication working in parallel to provide input, feedback, and refinement to each subsequent step, similar to the way machine learning works, the interpretations and conclusions of these studies would have been incomplete, if not incorrect. Further, the up-front time commitment to data processing and analytics above the time dedicated to place-based studies increased the productivity of the team and the impact of the research, unlike the common perception that cross-site research is often less efficient. In turn, this approach supported a non-traditional system of credit for co-authors based on citation impact of the journal selected as the publication outlet with less regard for author order. The LTAR network has embraced this modified scientific method in its shared research strategy and common experiment to address the problematic issues of mixed data quality across studies and sites, co-author credit, research efficiency, and scientific impact on data-intensive research. This approach can be combined with other types of collaborative and social media approaches, such as crowdsourcing, to take advantage of the wide range of expertise in the agroecological community as well as other disciplines to move science forward in the time of big data. C1 [Moran, M. Susan; Heilman, Philip; Collins, Chandra Holifield] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Peters, Debra P. C.] New Mexico State Univ, USDA ARS, Jornada Expt Range & Jornada Basin Long Term Ecol, Las Cruces, NM 88003 USA. RP Moran, MS (reprint author), USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. EM sumoran54@gmail.com NR 33 TC 0 Z9 0 U1 21 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01493 DI 10.1002/ecs2.1493 PG 11 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300032 ER PT J AU Riley, KL Grenfell, IC Finney, MA AF Riley, Karin L. Grenfell, Isaac C. Finney, Mark A. TI Mapping forest vegetation for the western United States using modified random forests imputation of FIA forest plots SO ECOSPHERE LA English DT Article DE Forest Inventory Analysis; imputation; LANDFIRE; random forests; tree list ID NEAREST-NEIGHBOR IMPUTATION; LIDAR DATA; WILDLAND FUELS; INVENTORY DATA; LEVEL; BIOMASS; USA; INFORMATION; SIMULATION; MANAGEMENT AB Maps of the number, size, and species of trees in forests across the western United States are desirable for many applications such as estimating terrestrial carbon resources, predicting tree mortality following wildfires, and for forest inventory. However, detailed mapping of trees for large areas is not feasible with current technologies, but statistical methods for matching the forest plot data with biophysical characteristics of the landscape offer a practical means to populate landscapes with a limited set of forest plot inventory data. We used a modified random forests approach with Landscape Fire and Resource Management Planning Tools (LANDFIRE) vegetation and biophysical predictors to impute plot data collected by the US Forest Service's Forest Inventory Analysis (FIA) to the landscape at 30-m grid resolution. This method imputes the plot with the best statistical match, according to a "forest" of decision trees, to each pixel of gridded landscape data. In this work, we used the LANDFIRE data set for gridded input because it is publicly available, offers seamless coverage of variables needed for fire models, and is consistent with other data sets, including burn probabilities and flame length probabilities generated for the continental United States. The main output of this project is a map of imputed plot identifiers at 30 x 30 m spatial resolution for the western United States that can be linked to the FIA databases to produce tree-level maps or to map other plot attributes. In addition, we used the imputed inventory data to generate maps of forest cover, forest height, and vegetation group at 30 x 30 m resolution for all forested pixels in the western United States, as a means of assessing the accuracy of our methodology. The results showed good correspondence between the target LANDFIRE data and the imputed plot data, with an overall within-class agreement of 79% for forest cover, 96% for forest height, and 92% for vegetation group. C1 [Riley, Karin L.] US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, 800 East Beckwith, Missoula, MT 59801 USA. [Grenfell, Isaac C.; Finney, Mark A.] US Forest Serv, Rocky Mt Res Stn, Missoula Fire Sci Lab, 5775 Highway 10 West, Missoula, MT 59801 USA. RP Riley, KL (reprint author), US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, 800 East Beckwith, Missoula, MT 59801 USA. EM kriley@fs.fed.us FU Rocky Mountain Research Station; National Fire Decision Support Center FX The Rocky Mountain Research Station and the National Fire Decision Support Center supported this effort. We are grateful to Nicholas Crookston for assistance in understanding and using his yaImpute package in R. Elizabeth Burrill at FIA and Chris Toney with the USFS Fire Laboratory in Missoula assisted us with obtaining FIA and LFRDB data via a Memorandum of Cooperation. We would also like to thank two anonymous reviewers for their comments on a previous draft. NR 36 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD OCT PY 2016 VL 7 IS 10 AR e01472 DI 10.1002/ecs2.1472 PG 22 WC Ecology SC Environmental Sciences & Ecology GA EB2TU UT WOS:000387216300014 ER PT J AU Kramer, MH Paparozzi, ET Stroup, AW AF Kramer, Matthew H. Paparozzi, Ellen T. Stroup, Andwalter W. TI Statistics in a Horticultural Journal: Problems and Solutions SO HORTTECHNOLOGY LA English DT Article DE experiment design; analysis; research planning; manuscript preparation AB We examined all articles in volume 139 and the first issue of volume 140 of the Journal of the American Society for Horticultural Science (JASHS) for statistical problems. Slightly fewer than half appeared to have problems. This is consistent with what has been found for other biological journals. Problems ranged from inappropriate analyses and statistical procedures to insufficient ( or complete lack of) information on how the analyses were performed. A common problem arose from taking many measurements from the same plant, which leads to correlated test results, ignored when declaring significance at P = 0.05 for each test. In this case, experiment-wise error control is lacking. We believe that many of these problems could and should have been caught in the writing or review process; i.e., identifying them did not require an extensive statistics background. This suggests that authors and reviewers have not absorbed nor kept current with many of the statistical basics needed for understanding their own data, for conducting proper statistical analyses, and for communicating their results. For a variety of reasons, graduate training in statistics for horticulture majors appears inadequate; we suggest that researchers in this field actively seek out opportunities to improve and update their statistical knowledge throughout their careers and engage a statistician as a collaborator early when unfamiliar methods are needed to design or analyze a research study. In addition, the ASHS, which publishes three journals, should assist authors, reviewers, and editors by recognizing and supporting the need for continuing education in quantitative literacy. C1 [Kramer, Matthew H.] ARS, USDA, Stat Grp, Bldg 005,Room 130,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Paparozzi, Ellen T.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Stroup, Andwalter W.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. RP Kramer, MH (reprint author), ARS, USDA, Stat Grp, Bldg 005,Room 130,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM matt.kramer@ars.usda.gov NR 18 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD OCT PY 2016 VL 26 IS 5 BP 558 EP 564 PG 7 WC Horticulture SC Agriculture GA EB5DH UT WOS:000387393100001 ER PT J AU Altland, JE Jeong, KY AF Altland, James E. Jeong, Kay Yeon TI Dolomitic Lime Amendment Affects Pine Bark Substrate pH, Nutrient Availability, and Plant Growth: A Review SO HORTTECHNOLOGY LA English DT Review DE calcium; magnesium; nitrogen; nitrate; ammonium; micronutrients ID DOUGLAS-FIR BARK; PEACH SEEDLINGS; LIMING RATE; LIMESTONE; CALCIUM AB Dolomitic lime (DL) is one of the most commonly used fertilizer amendments in nursery container substrates. It is used to adjust pH of pine bark substrates from their native pH, 4.1 to 5.1, up to about pH 6. However, additions of DL have been shown to be beneficial, inconsequential, or detrimental depending on the crop to which it is applied and irrigation water quality. Carbonate ions from DL cause a rate-dependent change in pH. Dolomitic lime can adjust pH of pine bark up to approximate to 6.5, after which there is little change regardless of how much additional DL is added. Changes in pH affect the rate of nitrification in pine bark substrates. The rate of nitrification can impact the quality of some plants that are sensitive to ammonium toxicity, as well as affect nitrogen leaching from containers. Changes in pH also affect micronutrient availability in pine bark substrates. Dolomitic lime provides an abundant source of calcium (Ca) and magnesium (Mg) for plant uptake. However, the additional Ca and Mg might also suppress potassium uptake in plants. C1 [Altland, James E.] ARS, USDA, Applicat Technol Res Unit, 1680 Madison Ave, Wooster, OH 44691 USA. [Jeong, Kay Yeon] Scotts Miracle Gro Co, 14111 Scottslawn Rd, Marysville, OH 43041 USA. RP Altland, JE (reprint author), ARS, USDA, Applicat Technol Res Unit, 1680 Madison Ave, Wooster, OH 44691 USA. EM james.altland@ars.usda.gov NR 40 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD OCT PY 2016 VL 26 IS 5 BP 565 EP 573 DI 10.21273/HORTTECH03465-16 PG 9 WC Horticulture SC Agriculture GA EB5DH UT WOS:000387393100002 ER PT J AU Russell, ES Liu, HP Gao, ZM Lamb, B Wagenbrenner, N AF Russell, Eric S. Liu, Heping Gao, Zhongming Lamb, Brian Wagenbrenner, Natalie TI Turbulence dependence on winds and stability in a weak-wind canopy sublayer over complex terrain SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE canopy sublayer turbulence; weak-wind conditions; canopy flow over complex terrain ID NOCTURNAL BOUNDARY-LAYER; PINE FOREST; INTERMITTENT TURBULENCE; COHERENT STRUCTURES; FLUX MEASUREMENTS; HARDWOOD FOREST; FLOWS; CASES-99; DENSITY; REGIMES AB The daytime and nighttime turbulence profiles within a weak-wind forest canopy were investigated by using data collected within a temperate mixed conifer canopy in northern Idaho, USA. Turbulence measurements made at three heights on a single tower within a Douglas fir canopy were compared. Data were split between the daytime and nighttime to determine the relationships among the local temperature gradient, wind direction, wind speed, and turbulence levels. The total flow field distributions and vertical statistical profiles were determined for the overnight and daytime periods to observe how the overall flow changed with time of day. During the day, the wind probability distribution function was consistent between heights but depended on the canopy depth overnight. The skewness changed with the dominant wind direction. The kurtosis increased with depth into the canopy and from during the day to overnight. The range of wind speeds observed was higher under unstable conditions than stable conditions. Daytime turbulence had no dependence on wind direction. Overnight, the relationship between turbulence and wind speed changed with wind direction and canopy depth. The highest turbulence values were associated with downslope winds near the canopy top, but the wind direction for the highest turbulence was variable within the trunk space. C1 [Russell, Eric S.; Liu, Heping; Gao, Zhongming; Lamb, Brian] Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. [Wagenbrenner, Natalie] US Forest Serv, Rocky Mt Res Stn, Missoula Fire Sci Lab, Missoula, MT USA. RP Liu, HP (reprint author), Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. EM heping.liu@wsu.edu FU National Science Foundation AGS [1419614, 1112938] FX We thank Qianyu Zhang, Yulong Ma, and Alejandro Pietro for their assistance with the field work. We also thank Bob Denner, USFS, the manager and care-taker of PREF during the study period. Comments from Jielun Sun, Justine Missik, and two anonymous reviewers greatly improved this work. We acknowledge support by National Science Foundation AGS under grants 1419614 and 1112938. The data used in this study are available for free when requested to H. L (heping.liu@wsu.edu). NR 60 TC 0 Z9 0 U1 7 U2 7 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD OCT PY 2016 VL 121 IS 19 BP 11502 EP 11515 DI 10.1002/2016JD025057 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA EA9PK UT WOS:000386976100038 ER PT J AU Rice, CP Bialek, K Hapeman, CJ McCarty, GW AF Rice, Clif'ford P. Bialek, Krystyna Hapeman, Cathleen J. McCarty, Gregory W. TI ROLE OF RIPARIAN AREAS IN ATMOSPHERIC PESTICIDE DEPOSITION AND ITS POTENTIAL EFFECT ON WATER QUALITY SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE pesticide fate and transport; precipitation; riparian buffers; environmental sampling; volatility; throughfall; stemflow ID CHESAPEAKE BAY; THROUGHFALL DEPOSITION; ORGANIC POLLUTANTS; COASTAL-PLAIN; SPRAY DRIFT; FOREST; BUFFERS; VOLATILIZATION; RAINFALL; CANOPY AB Riparian buffers are known to mitigate hydrologic losses of nutrients and other contaminants as they exit agricultural fields. The vegetation of riparian buffers can also trap atmospheric contaminants, and these pollutants can subsequently be delivered via rain to the riparian buffer floor. These processes, however, are poorly understood especially for pesticide residues. Therefore, we conducted a four-year study examining stemflow and throughfall to a riparian buffer which was adjacent a cultured Zea mays field treated with atrazine and metolachlor. Stemflow is rain contacting the tree canopy traveling down smaller to larger branches and down the tree trunk, whereas throughfall is rain that may or may not contact leaves and branches and reaches the earth. Stemflow concentrations of the herbicides were larger than throughfall concentrations and accounted for 5-15% of the atrazine and 6-66% of the metolachlor depositional fluxes under the canopy. Larger depositional fluxes were measured when leaves were more fully emerged and temperatures and humidity were elevated. Rain collected outside the riparian buffer on the field side and on the back side revealed the trees trapped the herbicide residues. Herbicide loading to the riparian buffer stream was found to be linked to tree canopy deposition and subsequent washoff during rain events. These results indicate that in agricultural areas canopy washoff can be an important source of pesticides to surface waters. C1 [Rice, Clif'ford P.; Bialek, Krystyna] ARS, Sustainable Agr Syst Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Hapeman, Cathleen J.; McCarty, Gregory W.] ARS, Hydrol & Remote Sensing Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Rice, CP (reprint author), ARS, Sustainable Agr Syst Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM clifford.rice@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service FX Mention of specific products is for identification and does not imply endorsement by the U.S. Department of Agriculture to the exclusion of other suitable products or suppliers. This work was supported financially by U.S. Department of Agriculture, Agricultural Research Service intramural projects in National Programs 211: Water Availability and Water Management, and 212: Soil and Air. NR 45 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD OCT PY 2016 VL 52 IS 5 BP 1109 EP 1120 DI 10.1111/1752-1688.12444 PG 12 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2EL UT WOS:000387170400008 ER PT J AU Tullos, DD Collins, MJ Bellmore, JR Bountry, JA Connolly, PJ Shafroth, PB Wilcox, AC AF Tullos, Desiree D. Collins, MathMathias J. Bellmore, J. Ryan Bountry, Jennifer A. Connolly, Patrick J. Shafroth, Patrick B. Wilcox, Andrew C. TI SYNTHESIS OF COMMON MANAGEMENT CONCERNS ASSOCIATED WITH DAM REMOVAL SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE sediment management; headcut; aggradation; reservoir erosion; reservoir drawdown; wells; turbidity; nonnative plants; invasive fish; dam removal; river restoration ID LOW-HEAD DAM; WHITE SALMON RIVER; FRESH-WATER FISH; ELWHA RIVER; VEGETATION DEVELOPMENT; PACIFIC-NORTHWEST; CONDIT DAM; SEDIMENT; CHANNEL; WASHINGTON AB Managers make decisions regarding if and how to remove dams in spite of uncertainty surrounding physical and ecological responses, and stakeholders often raise concerns about certain negative effects, regardless of whether these concerns are warranted at a particular site. We used a dam-removal science database supplemented with other information sources to explore seven frequently raised concerns, herein Common Management Concerns (CMCs). We investigate the occurrence of these concerns and the contributing biophysical controls. The CMCs addressed are the following: degree and rate of reservoir sediment erosion, excessive channel incision upstream of reservoirs, downstream sediment aggradation, elevated downstream turbidity, drawdown impacts on local water infrastructure, colonization of reservoir sediments by nonnative plants, and expansion of invasive fish. Biophysical controls emerged for some of the concerns, providing managers with information to assess whether a given concern is likely to occur at a site. To fully assess CMC risk, managers should concurrently evaluate site conditions and identify the ecosystem or human uses that will be negatively affected if the biophysical phenomenon producing the CMC occurs. We show how many CMCs have one or more controls in common, facilitating the identification of multiple risks at a site, and demonstrate why CMC risks should be considered in the context of other factors such as natural watershed variability and disturbance history. C1 [Tullos, Desiree D.] Oregon State Univ, Biol & Ecol Engn Dept, 116 Gilmore Hall, Corvallis, OR 97331 USA. [Collins, MathMathias J.] NOAA, Restorat Ctr, Natl Marine Fisheries Serv, Gloucester, MA 01930 USA. [Bellmore, J. Ryan] US Forest Serv, Pacific Northwest Res Stn, Juneau, AK 99801 USA. [Bountry, Jennifer A.] US Bur Reclamat, Sedimentat & River Hydraul Grp, Lakewood, CO 80225 USA. [Connolly, Patrick J.] US Geol Survey, Western Fisheries Res Ctr, Cook, WA 98605 USA. [Shafroth, Patrick B.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Wilcox, Andrew C.] Univ Montana, Dept Geosci, Missoula, MT 59812 USA. RP Tullos, DD (reprint author), Oregon State Univ, Biol & Ecol Engn Dept, 116 Gilmore Hall, Corvallis, OR 97331 USA. EM desiree.tullos@oregonstate.edu OI Collins, Mathias/0000-0003-4238-2038; Wilcox, Andrew C./0000-0002-6241-8977 NR 138 TC 0 Z9 0 U1 33 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD OCT PY 2016 VL 52 IS 5 BP 1179 EP 1206 DI 10.1111/1752-1688.12450 PG 28 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2EL UT WOS:000387170400013 ER PT J AU Harmel, DR Christianson, LE McBroom, MW Smith, DR Higgs, KD AF Harmel, Daren R. Christianson, Laura E. McBroom, Matthew W. Smith, Douglas R. Higgs, Kori D. TI EXPANSION OF THE MANAGE DATABASE WITH FOREST AND DRAINAGE STUDIES SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE nitrogen; phosphorus; water quality; nonpoint source pollution; drainage; forest hydrology ID WATER-QUALITY; NUTRIENT; NITROGEN; IMPACTS; LOAD AB The "Measured Annual Nutrient loads from AGricultural Environments" (MANAGE) database was published in 2006 to expand an early 1980s compilation of nutrient export (load) data from cultivated and pasture/range land at the field or farm scale. Then in 2008, MANAGE was updated with 15 additional studies, and nitrogen (N) and phosphorus (P) concentrations in runoff were added. Since then, MANAGE has undergone significant expansion adding N and P water quality along with relevant management and site characteristic data from: (1) 30 runoff studies from forested land uses, (2) 91 drainage water quality studies from drained land, and (3) 12 additional runoff studies from cultivated and pasture/range land uses. In this expansion, an application timing category was added to the existing fertilizer data categories (rate, placement, formulation) to facilitate analysis of 4R Nutrient Stewardship, which emphasizes right fertilizer source, rate, time, and place. In addition, crop yield and N and P uptake data were added, although this information was only available for 21 and 7% of studies, respectively. Inclusion of these additional data from cultivated, pasture/range, and forest land uses as well as artificially drained agricultural land should facilitate expanded spatial analyses and improved understanding of regional differences, management practice effectiveness, and impacts of land use conversions and management techniques. The current version is available at www.ars.usda.gov/spa/manage-nutrient. C1 [Harmel, Daren R.; Smith, Douglas R.] ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. [Christianson, Laura E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [McBroom, Matthew W.] Stephen F Austin Univ, Coll Forestry & Agr, Nacogdoches, TX 75925 USA. [Higgs, Kori D.] North Carolina A&T Univ, Biol Engn Dept, Greensboro, NC 27411 USA. RP Harmel, DR (reprint author), ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. EM daren.harmel@ars.usda.gov FU Texas State Soil and Water Conservation Board; 4R Research Fund FX Rehanon Pampell was a tremendous help preparing this manuscript, for publication. The Texas State Soil and Water Conservation Board is recognized for funding the original development of MANAGE and for its dedicated commitment to put effective conservation on the ground. The authors also gratefully acknowledge the 4R Research Fund for supporting a portion of this work. NR 14 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD OCT PY 2016 VL 52 IS 5 BP 1275 EP 1279 DI 10.1111/1752-1688.12438 PG 5 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2EL UT WOS:000387170400018 ER PT J AU Gaguancela, OA Zuniga, LP Arias, AV Halterman, D Flores, FJ Johansen, IE Wang, A Yamaji, Y Verchot, J AF Gaguancela, Omar Arias Zuniga, Lizbeth Pena Arias, Alexis Vela Halterman, Dennis Flores, Francisco Javier Johansen, Ida Elisabeth Wang, Aiming Yamaji, Yasuyuki Verchot, Jeanmarie TI The IRE1/bZIP60 Pathway and Bax Inhibitor 1 Suppress Systemic Accumulation of Potyviruses and Potexviruses in Arabidopsis and Nicotiana benthamiana Plants SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID UNFOLDED PROTEIN RESPONSE; ENDOPLASMIC-RETICULUM STRESS; PROGRAMMED CELL-DEATH; TURNIP-MOSAIC-VIRUS; REGULATED IRE1-DEPENDENT DECAY; DEPENDENT RNA-POLYMERASE; F-BOX PROTEINS; ER STRESS; VIRAL REPLICATION; MESSENGER-RNA AB The inositol requiring enzyme (IRE1) is an endoplasmic reticulum (ER) stress sensor. When activated, it splices the bZIP60 mRNA, producing a truncated transcription factor that upregulates genes involved in the unfolded protein response. Bax inhibitor 1 (BI-1) is another ER stress sensor that regulates cell death in response to environmental assaults. The potyvirus 6K2 and potexvirus TGB3 proteins are known to reside in the ER, serving, respectively, as anchors for the viral replicase and movement protein complex. This study used green fluorescent protein (GFP)-tagged Turnip mosaic virus (TuMV), Plantago asiatica mosaic virus (PIAMV), Potato virus Y (PVY), and Potato virus X (PVX) to determine that the IRE1/bZIP60 pathway and BI-1 machinery are induced early in virus infection in Arabidopsis thaliana, Nicotiana benthamiana, and Solanum tuberosum. Agrodelivery of only the potyvirus 6K2 or TGB3 genes into plant cells activated bZIP60 and BI-1 expression in Arabidopsis thaliana, N. benthamiana, and S. tuberosum. Homozygous ire1a-2, ire1b-4, and ire1a-2/ire1b-4 mutant Arabidopsis plants were inoculated with TuMV-GFP or PlAMV-GFP. PIAMV accumulates to a higher level in ire1a-2 or ire1a-2/ire1b-4 mutant plants than in ire1b-4 or wild-type plants. TuMV-GFP accumulates to a higher level in ire1a-2, ire1b-4, or ire1a-2/ire1b-4 compared with wild-type plants, suggesting that both isoforms contribute to TuMV-GFP infection. Gene silencing was used to knock down bZIP60 and BI-1 expression in N. benthamiana. PVX-GFP and PVY-GFP accumulation was significantly elevated in these silenced plants compared with control plants. This study demonstrates that two ER stress pathways, namely IRE1/bZIP60 and the BI-1 pathway, limit systemic accumulation of potyvirus and potexvirus infection. Silencing BI-1 expression also resulted in systemic necrosis. These data suggest that ER stress activated pathways, led by IRE1 and BI-1, respond to invading potyvirus and potexviruses to restrict virus infection and enable physiological changes enabling plants to tolerate virus assault. C1 [Gaguancela, Omar Arias; Zuniga, Lizbeth Pena; Verchot, Jeanmarie] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Arias, Alexis Vela; Flores, Francisco Javier] Univ Fuerzas Armadas ESPE, Dept Ciencias Vida & Agr, Ave Gen Ruminahui S-N, Sangolqui, Pichincha, Ecuador. [Halterman, Dennis] ARS, Vegetable Crops Res Unit, USDA, Madison, WI USA. [Johansen, Ida Elisabeth] Univ Copenhagen, Dept Plant & Environm Sci, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. [Wang, Aiming] AAFC, Southern Crop Protect & Food Res Ctr, 1391 Sandford St, London, ON N5V 4T3, Canada. [Yamaji, Yasuyuki] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Agr & Environm Biol, Lab Plant Pathol,Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan. RP Verchot, J (reprint author), Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. EM Verchot.lubicz@okstate.edu FU National Institute of Food and Agriculture Plant Associated Microbes and Plant-Microbe Interactions [2014-67014-21715] FX Special thanks to S. Namba for valuable conversations. This research was funded by the National Institute of Food and Agriculture Plant Associated Microbes and Plant-Microbe Interactions award number 2014-67014-21715. NR 74 TC 1 Z9 1 U1 11 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD OCT PY 2016 VL 29 IS 10 BP 750 EP 766 DI 10.1094/MPMI-07-16-0147-R PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA EB3XA UT WOS:000387301200002 PM 27578623 ER PT J AU Tundo, S Janni, M Moscetti, I Mandala, G Savatin, D Blechl, A Favaron, F D'Ovidio, R AF Tundo, Silvio Janni, Michela Moscetti, Ilaria Mandala, Giulia Savatin, Daniel Blechl, Ann Favaron, Francesco D'Ovidio, Renato TI PvPGIP2 Accumulation in Specific Floral Tissues But Not in the Endosperm Limits Fusarium graminearum Infection in Wheat SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID TRANSGENIC WHEAT; SPRING WHEAT; EXPRESSION; RESISTANCE; BARLEY; PROMOTER; GENE; PROTEIN; SPIKES; PLANTS AB Fusarium head blight (FHB) caused by Fusarium graminearum is one of the most destructive fungal diseases of wheat worldwide. The pathogen infects the spike at flowering time and causes severe yield losses, deterioration of grain quality, and accumulation of mycotoxins. The understanding of the precise means of pathogen entry and colonization of floral tissue is crucial to providing effective protection against FHB. Polygalacturonase (PG) inhibiting proteins (PGIPs) are cell-wall proteins that inhibit the activity of PGs, a class of pectin-depolymerizing enzymes secreted by microbial pathogens, including Fusarium spp. The constitutive expression of a bean PGIP (PvPGIP2) limits FHB symptoms and reduces mycotoxin accumulation in wheat grain. To better understand which spike tissues play major roles in limiting F. graminearum infection, we explored the use of PvPGIP2 to defend specific spike tissues. We show here that the simultaneous expression of PvPGIP2 in lemma, palea, rachis, and anthers reduced FHB symptoms caused by F. graminearum compared with symptoms in infected nontransgenic plants. However, the expression of PvPGIP2 only in the endosperm did not affect FHB symptom development, indicating that once the pathogen has reached the endosperm, inhibition of the pathogen's PG activity is not effective in preventing its further spread. C1 [Tundo, Silvio; Janni, Michela; Moscetti, Ilaria; Mandala, Giulia; D'Ovidio, Renato] Univ Tuscia, Dipartimento Sci Agr & Forestali DAFNE, Via S Camillo de Lellis Snc, I-01100 Viterbo, Italy. [Savatin, Daniel] Univ Roma La Sapienza, Dipartimento Biol & Biotecnol Charles Darwin, Piazzale Aldo Moro 5, I-00185 Rome, Italy. [Blechl, Ann] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Favaron, Francesco] Univ Padua, Dipartimento Terr & Sistemi AgroForestali TeSAF, Res Grp Plant Pathol, Viale Univ 16, I-35020 Legnaro, PD, Italy. [Janni, Michela] CNR, IMEM, Parco Area Sci 37-A, I-43124 Parma, Italy. [Moscetti, Ilaria] Univ Tuscia, Dipartimento Sci Ecol & Biol DEB, I-01100 Viterbo, Italy. RP D'Ovidio, R (reprint author), Univ Tuscia, Dipartimento Sci Agr & Forestali DAFNE, Via S Camillo de Lellis Snc, I-01100 Viterbo, Italy. EM dovidio@unitus.it OI Savatin, Daniel-Valentin/0000-0002-2165-1369 FU Italian Ministry of University and Research; Italian Ministry of Agricultural, Food and Forestry Policies; Regione Lazio FX Research was supported by the Italian Ministry of University and Research (grant Prin 2010-2011) and the Italian Ministry of Agricultural, Food and Forestry Policies (grant Progetto Internazionale di Sequenziamento del Genoma di Frumenti, Mappa fisica del Cromosoma 5A). M. Janni and S. Tundo were supported by 'Regione Lazio' (grant 'Ricercatori a Tempo Determinato'). The United States Department of Agriculture (USDA) is an equal opportunity provider and employer. Mention of a specific product name by the USDA does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 32 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD OCT PY 2016 VL 29 IS 10 BP 815 EP 821 DI 10.1094/MPMI-07-16-0148-R PG 7 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA EB3XA UT WOS:000387301200008 PM 27671121 ER PT J AU Cao, HP Sethumadhavan, K AF Cao, Heping Sethumadhavan, Kandan TI Phosphopeptide mapping and mass spectrometry analysis of human tristetraprolin SO PROTEIN SCIENCE LA English DT Meeting Abstract CT 30th Anniversary Symposium of the Protein-Society CY JUL 16-19, 2016 CL Baltimore, MD SP Protein Soc C1 [Cao, Heping; Sethumadhavan, Kandan] ARS, USDA, Southern Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 EI 1469-896X J9 PROTEIN SCI JI Protein Sci. PD OCT PY 2016 VL 25 SU S1 BP 170 EP 170 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EB1YJ UT WOS:000387152400302 ER PT J AU Cao, HP Deterding, LJ Blackshear, PJ AF Cao, Heping Deterding, Leesa J. Blackshear, Perry J. TI Characterization of protein compositions of glanded and glandless cotton seeds SO PROTEIN SCIENCE LA English DT Meeting Abstract CT 30th Anniversary Symposium of the Protein-Society CY JUL 16-19, 2016 CL Baltimore, MD SP Protein Soc C1 [Cao, Heping] ARS, USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Deterding, Leesa J.] NIEHS, Lab Struct Biol, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Blackshear, Perry J.] NIEHS, Lab Signal Transduct, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. [Blackshear, Perry J.] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA. [Blackshear, Perry J.] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 EI 1469-896X J9 PROTEIN SCI JI Protein Sci. PD OCT PY 2016 VL 25 SU S1 BP 174 EP 174 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA EB1YJ UT WOS:000387152400309 ER PT J AU Sato, LY Gomes, VCF Shimabukuro, YE Keller, M Arai, E Nara dos-Santos, M Brown, IF de Aragao, LEOEC AF Sato, Luciane Yumie Faria Gomes, Vitor Conrado Shimabukuro, Yosio Edemir Keller, Michael Arai, Egidio Nara dos-Santos, Maiza Brown, Irving Foster Oliveira e Cruz de Aragao, Luiz Eduardo TI Post-Fire Changes in Forest Biomass Retrieved by Airborne LiDAR in Amazonia SO REMOTE SENSING LA English DT Article DE light detection and ranging; Amazon; aboveground biomass; tropical forest; fire; LiDAR; degradation ID TREE REGENERATION RESPONSES; SMALL-FOOTPRINT LIDAR; ABOVEGROUND BIOMASS; BRAZILIAN AMAZON; NEOTROPICAL FOREST; FIRE BEHAVIOR; TROPICAL FORESTS; BAMBOO DIEBACK; CARBON STOCKS; DEFORESTATION AB Fire is one of the main factors directly impacting Amazonian forest biomass and dynamics. Because of Amazonia's large geographical extent, remote sensing techniques are required for comprehensively assessing forest fire impacts at the landscape level. In this context, Light Detection and Ranging (LiDAR) stands out as a technology capable of retrieving direct measurements of vegetation vertical arrangement, which can be directly associated with aboveground biomass. This work aims, for the first time, to quantify post-fire changes in forest canopy height and biomass using airborne LiDAR in western Amazonia. For this, the present study evaluated four areas located in the state of Acre, called Rio Branco, Humaita, Bonal and Talisma. Rio Branco and Humaita burned in 2005 and Bonal and Talisma burned in 2010. In these areas, we inventoried a total of 25 plots (0.25 ha each) in 2014. Humaita and Talisma are located in an open forest with bamboo and Bonal and Rio Branco are located in a dense forest. Our results showed that even ten years after the fire event, there was no complete recovery of the height and biomass of the burned areas (p < 0.05). The percentage difference in height between control and burned sites was 2.23% for Rio Branco, 9.26% for Humaita, 10.03% for Talisma and 20.25% for Bonal. All burned sites had significantly lower biomass values than control sites. In Rio Branco (ten years after fire), Humaita (nine years after fire), Bonal (four years after fire) and Talisma (five years after fire) biomass was 6.71%, 13.66%, 17.89% and 22.69% lower than control sites, respectively. The total amount of biomass lost for the studied sites was 16,706.3 Mg, with an average loss of 4176.6 Mg for sites burned in 2005 and 2890 Mg for sites burned in 2010, with an average loss of 3615 Mg. Fire impact associated with tree mortality was clearly detected using LiDAR data up to ten years after the fire event. This study indicates that fire disturbance in the Amazon region can cause persistent above-ground biomass loss and subsequent reduction of forest carbon stocks. Continuous monitoring of burned forests is required for depicting the long-term recovery trajectory of fire-affected Amazonian forests. C1 [Sato, Luciane Yumie; Shimabukuro, Yosio Edemir; Arai, Egidio; Oliveira e Cruz de Aragao, Luiz Eduardo] Natl Inst Space Res, Remote Sensing Div, Ave Astronautas 1758, BR-12227010 Sao Paulo, Brazil. [Faria Gomes, Vitor Conrado] Trevo Coronel Aviador Jose Alberto Albano Amarant, Dept Sci & Aerosp Technol, BR-12228001 Sao Paulo, Brazil. [Keller, Michael] US Forest Serv, USDA, Int Inst Trop Forestry, 1201 Calle Ceiba Jardin Bot Sur, Rio Piedra, PR 00926 USA. [Keller, Michael; Nara dos-Santos, Maiza] Embrapa Informat Agr, Ave Doutor Andre Tosello,209 Cidade Univ, BR-13083886 Sao Paulo, Brazil. [Brown, Irving Foster] Woods Hole Res Ctr, Parque Zoobot, BR-69915900 Rio Branco, Acre, Brazil. [Brown, Irving Foster] Univ Fed Acre UFAC, Parque Zoobot, BR-69915900 Rio Branco, Acre, Brazil. [Oliveira e Cruz de Aragao, Luiz Eduardo] Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4SB, Devon, England. RP Sato, LY; de Aragao, LEOEC (reprint author), Natl Inst Space Res, Remote Sensing Div, Ave Astronautas 1758, BR-12227010 Sao Paulo, Brazil.; de Aragao, LEOEC (reprint author), Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4SB, Devon, England. EM lucianesato@gmail.com; vitor@ieav.cta.br; yosio@dsr.inpe.br; mkeller.co2@gmail.com; egidio@dsr.inpe.br; maizanara@gmail.com; fbrown@uol.com.br; laragao@dsr.inpe.br FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) through the National Institute for Space Research (INPE); UK Natural Environment Research Council (NERC) [NE/F015356/2, NE/l018123/1]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [304425/2013-3, 458022/2013-6]; Brazilian Agricultural Research Corporation (EMBRAPA); US Forest Service, USAID; US Department of State FX L.Y.S. thanks the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the graduate scholarship offered through the National Institute for Space Research (INPE). L.E.O.C.A. acknowledges the support of the UK Natural Environment Research Council (NERC) grants (NE/F015356/2 and NE/l018123/1) and the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) grants (304425/2013-3 and 458022/2013-6) Data were acquired by the Sustainable Landscapes Brazil project supported by the Brazilian Agricultural Research Corporation (EMBRAPA), the US Forest Service, USAID, and the US Department of State. NR 77 TC 0 Z9 0 U1 10 U2 10 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD OCT PY 2016 VL 8 IS 10 AR 839 DI 10.3390/rs8100839 PG 15 WC Remote Sensing SC Remote Sensing GA EB4QI UT WOS:000387357300053 ER PT J AU Klotz, JL Aiken, GE Bussard, JR Foote, AP Harmon, DL Goff, BM Schrick, FN Strickland, JR AF Klotz, James L. Aiken, Glen E. Bussard, Jessica R. Foote, Andrew P. Harmon, David L. Goff, Ben M. Schrick, F. Neal Strickland, James R. TI Vasoactivity and Vasoconstriction Changes in Cattle Related to Time off Toxic Endophyte-Infected Tall Fescue SO TOXINS LA English DT Article DE bovine; ergot alkaloids; tall fescue; vasoconstriction ID LATERAL SAPHENOUS VEINS; SEROTONIN RECEPTORS; CAUDAL ARTERY; ERGOPEPTINE ALKALOIDS; ERGOT ALKALOIDS; SERUM PROLACTIN; BEEF HEIFERS; WILD-TYPE; IN-VITRO; STEERS AB Previous research has indicated that serotonergic and ff -adrenergic receptors in peripheral vasculature are affected by exposure of cattle grazing toxic endophyte-infected (E+; Epichle coenophialia) tall fescue (Lolium arundinaceum). The objective of this experiment was to determine the period of time necessary for the vascular effects of ergot alkaloids to subside. Two experiments were conducted to investigate changes in vascular contractile response and vasoconstriction over time relative to removal from an ergot alkaloid-containing E+ tall fescue pasture. In Experiment 1, lateral saphenous vein biopsies were conducted on 21 predominantly Angus steers (357 +/- 3 kg body weight) at 0 (n = 6), 7 (n = 6), 14 (n = 5), or 28 days (n = 4) after removal from grazing pasture (3.0 ha; endpoint ergovaline + ergovalinine = 1.35 mg/kg DM) for 126 days. In Experiment 2, lateral saphenous veins were biopsied from 24 Angus-cross steers (361 +/- 4 kg body weight) at 0, 21, 42, and 63 days (n = 6 per time point) following removal from grazing tall fescue pastures (3.0 ha; first 88 days endpoint ergovaline + ergovalinine = 0.15 mg/kg DM; last 18 days endpoint ergovaline + ergovalinine = 0.57 mg/kg DM) for 106 total days. Six steers (370 +/- 18 kg body weight) off of bermudagrass pasture for the same time interval were also biopsied on Day 0 and Day 63 (n = 3 per time point). Additionally, in Experiment 2, cross-sectional ultrasound scans of caudal artery at the fourth coccygeal vertebra were taken on Days 0, 8, 15, 21, 29, 36, 42, and 45 to determine mean artery luminal area to evaluate vasoconstriction. In both experiments, steers were removed from pasture and housed in a dry lot and fed a corn silage diet for the duration of biopsies and ultrasound scans. Biopsied vessels used to evaluate vasoactivity were cleaned, incubated in a multimyograph, and exposed to increasing concentrations of 4-Bromo-3,6-dimethoxybenzocyclobuten-1-yl) methylamine hydrobromide (TCB2; 5HT(2A) agonist), guanfacine (GF; alpha(2A)-adrenergic agonist), and (R)-(+)-m-nitrobiphenyline oxalate (NBP; alpha(2C)-adrenergic agonist) in both experiments and ergovaline (ERV) and ergotamine (ERT) in Experiments 1 and 2, respectively. In Experiment 1, days off pasture x agonist concentration was not significant (p > 0.1) for all four compounds tested. In Experiment 2, GF, NBP, TCB2 and ERT were significant for days off pasture x agonist concentration interaction (p < 0.02) and vasoactivity increased over time. Vasoactivity to agonists was reduced (p < 0.05) when steers were initially removed from E+ tall fescue pasture compared to bermudagrass, but did not differ by Day 63 for any variable. Luminal areas of caudal arteries in steers grazed on E+ tall fescue relaxed and were similar to steers that had grazed bermudagrass for 36 days on non-toxic diet (p = 0.15). These data demonstrate changes in peripheral vasoactivity and recovery from vasoconstriction occur beyond five weeks off toxic pasture and 5HT2A receptors appear to be more dramatically affected in the lateral saphenous vein by grazing E+ tall fescue pasture than adrenergic receptors. C1 [Klotz, James L.; Aiken, Glen E.; Strickland, James R.] USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. [Bussard, Jessica R.; Goff, Ben M.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Foote, Andrew P.; Harmon, David L.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Schrick, F. Neal] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. [Foote, Andrew P.] Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Strickland, James R.] Clemson Univ, Dept Anim & Vet Sci, Clemson, SC 29634 USA. RP Klotz, JL (reprint author), USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. EM james.klotz@ars.usda.gov; glen.aiken@ars.usda.gov; jrb5218@gmail.com; andrew.foote@ars.usda.gov; dharmon@uky.edu; ben.goff@uky.edu; fschrick@utk.edu; jrstric@clemson.edu NR 49 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD OCT PY 2016 VL 8 IS 10 AR 271 DI 10.3390/toxins8100271 PG 19 WC Toxicology SC Toxicology GA EB4MZ UT WOS:000387347300003 ER PT J AU Sun, HL Xue, JL Bailey, E Xu, YF Hu, GL Baroch, J Zhang, Y Pace, L DeLiberto, TJ Wan, XF AF Sun, Hailiang Xue, Jian-Li Bailey, Elizabeth Xu, Yifei Hu, Guoliang Baroch, John Zhang, Yi Pace, Lanny DeLiberto, Thomas J. Wan, Xiu-Feng TI A quantitative RT-PCR assay for rapid detection of Eurasian-lineage H10 subtype influenza A virus SO VIROLOGICA SINICA LA English DT Letter ID UNITED-STATES; INFECTION; BIRDS; GENE; H5 C1 [Sun, Hailiang; Xue, Jian-Li; Bailey, Elizabeth; Xu, Yifei; Wan, Xiu-Feng] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Hu, Guoliang] Jiangxi Agr Univ, Coll Anim Sci & Technol, Nanchang 330045, Peoples R China. [Baroch, John; DeLiberto, Thomas J.] USDA, Anim & Plant Hlth Inspect Serv, Ft Collins, CO 80526 USA. [Zhang, Yi; Pace, Lanny] Mississippi State Univ, Coll Vet Med, Mississippi Vet Res & Diagnost Lab, Mississippi State, MS 39762 USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu FU NIGMS NIH HHS [P20 GM103646] NR 16 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1674-0769 EI 1995-820X J9 VIROL SIN JI Virol. Sin. PD OCT PY 2016 VL 31 IS 5 BP 444 EP 447 DI 10.1007/s12250-016-3826-1 PG 4 WC Virology SC Virology GA EB7ME UT WOS:000387570900009 PM 27699612 ER PT J AU Carlson, J O'Donnell, V Alfano, M Velazquez Salinas, L Holinka, LG Krug, PW Gladue, DP Higgs, S Borca, MV AF Carlson, Jolene O'Donnell, Vivian Alfano, Marialexia Velazquez Salinas, Lauro Holinka, Lauren G. Krug, Peter W. Gladue, Douglas P. Higgs, Stephen Borca, Manuel V. TI Association of the Host Immune Response with Protection Using a Live Attenuated African Swine Fever Virus Model SO VIRUSES-BASEL LA English DT Article DE ASF; ASFV; immune response ID VIRULENCE-ASSOCIATED GENE; DOMESTIC PIGS; CHALLENGE; ISOLATE; IMMUNIZATION; MACROPHAGES; ANTIBODIES; INFECTION; LEADS; SERA AB African swine fever (ASF) is a lethal hemorrhagic disease of swine caused by a double-stranded DNA virus, ASF virus (ASFV). There is no vaccine to prevent the disease and current control measures are limited to culling and restricting animal movement. Swine infected with attenuated strains are protected against challenge with a homologous virulent virus, but there is limited knowledge of the host immune mechanisms generating that protection. Swine infected with Pretoriuskop/96/4 (Pret4) virus develop a fatal severe disease, while a derivative strain lacking virulence-associated gene 9GL (Pret49GL virus) is completely attenuated. Swine infected with Pret49GL virus and challenged with the virulent parental virus at 7, 10, 14, 21, and 28 days post infection (dpi) showed a progressive acquisition of protection (from 40% at 7 dpi to 80% at 21 and 28 dpi). This animal model was used to associate the presence of host immune response (ASFV-specific antibody and interferon (IFN)- responses, or specific cytokine profiles) and protection against challenge. With the exception of ASFV-specific antibodies in survivors challenged at 21 and 28 dpi, no association between the parameters assessed and protection could be established. These results, encompassing data from 65 immunized swine, underscore the complexity of the system under study, suggesting that protection relies on the concurrence of different host immune mechanisms. C1 [Carlson, Jolene; O'Donnell, Vivian; Alfano, Marialexia; Velazquez Salinas, Lauro; Holinka, Lauren G.; Krug, Peter W.; Gladue, Douglas P.; Borca, Manuel V.] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Carlson, Jolene; Higgs, Stephen] Kansas State Univ, Biosecur Res Inst, Coll Vet Med, Manhattan, KS 66506 USA. [Carlson, Jolene; Higgs, Stephen] Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA. [O'Donnell, Vivian] Univ Connecticut, Dept Pathobiol, Storrs, CT 06269 USA. RP Borca, MV (reprint author), ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM Jolene.Carlson@ars.usda.gov; Vivian.odonnell@ars.usda.gov; Marialexia.Alfano@ars.usda.gov; Lauro.Velazquez@ars.usda.gov; Lauren.Holinka@ars.usda.gov; Peter.Krug@ars.usda.gov; Douglas.Gladue@ars.usda.gov; shiggs@bri.ksu.edu; manuel.borca@ars.usda.gov OI Gladue, Douglas/0000-0002-7894-0233 FU State of Kansas National Bio and Agro-Defense Facility Fund (NBAF); Institute for Infectious Animal Diseases (IIAD) Career Development Program; Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-11-X-00077, HSHQPM-12-X-00005]; ARS/USDA-University of Connecticut SCA [58-1940-1-190] FX We thank the Plum Island Animal Disease Center Animal Resource Branch for excellent technical assistance. We thank Michael Eschbaumer for his help with creating several of the figures in R. This project was partially funded through the State of Kansas National Bio and Agro-Defense Facility Fund (NBAF) and Institute for Infectious Animal Diseases (IIAD) Career Development Program, and an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Numbers HSHQDC-11-X-00077 and HSHQPM-12-X-00005. We also thank ARS/USDA-University of Connecticut SCA# 58-1940-1-190 for partially supporting this work. NR 29 TC 0 Z9 0 U1 0 U2 0 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4915 J9 VIRUSES-BASEL JI Viruses-Basel PD OCT PY 2016 VL 8 IS 10 AR 291 DI 10.3390/v8100291 PG 15 WC Virology SC Virology GA EB6HI UT WOS:000387482200026 ER PT J AU Yacoob, C Lange, M Cohen, K Lathia, K Feng, JL Glenn, J Davidson, J Carbonetti, S Oliver, B Vigdorovich, V Sather, DN Stamatatos, L AF Yacoob, Christina Lange, Miles Cohen, Kristen Lathia, Kanan Feng, Junli Glenn, Jolene Davidson, Jerry Carbonetti, Sara Oliver, Brian Vigdorovich, Vladimir Sather, D. Noah Stamatatos, Leonidas TI Envelope Immunogen Properties Direct Selective B Cell Expansion and Neutralizing Antibodies in HIV-1 Immunized Rhesus Macaques SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Meeting Abstract CT Conference on HIV Research for Prevention (HIV R4P) CY OCT 17-20, 2016 CL Chicago, IL C1 [Yacoob, Christina; Cohen, Kristen; Lathia, Kanan; Feng, Junli; Glenn, Jolene; Davidson, Jerry; Stamatatos, Leonidas] Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA. [Lange, Miles] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Washington, DC 20250 USA. [Carbonetti, Sara; Oliver, Brian; Vigdorovich, Vladimir; Sather, D. Noah] Ctr Infect Dis Res, Rockville, MD USA. [Stamatatos, Leonidas] Univ Washington, Seattle, WA 98195 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 0889-2229 EI 1931-8405 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD OCT PY 2016 VL 32 SU 1 MA P01.04 BP 133 EP 133 PG 1 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA EA6YH UT WOS:000386774600234 ER PT J AU Clark, LV Dzyubenko, E Dzyubenko, N Bagmet, L Sabitov, A Chebukin, P Johnson, DA Kjeldsen, JB Petersen, KK Jorgensen, U Yoo, JH Heo, K Yu, CY Zhao, H Jin, XL Peng, JH Yamada, T Sacks, EJ AF Clark, Lindsay V. Dzyubenko, Elena Dzyubenko, Nikolay Bagmet, Larisa Sabitov, Andrey Chebukin, Pavel Johnson, Douglas A. Kjeldsen, Jens Bonderup Petersen, Karen Koefoed Jorgensen, Uffe Yoo, Ji Hye Heo, Kweon Yu, Chang Yeon Zhao, Hua Jin, Xiaoli Peng, Junhua Yamada, Toshihiko Sacks, Erik J. TI Ecological characteristics and in situ genetic associations for yield-component traits of wild Miscanthus from eastern Russia SO ANNALS OF BOTANY LA English DT Article DE Chloroplast; genome-wide association analysis (GWAS); germplasm; Miscanthus sacchariflorus; Miscanthus sinensis; population genetics; restriction site-associated DNA sequencing (RAD-seq); Russia; single nucleotide polymorphism (SNP) ID GENOME-WIDE ASSOCIATION; POPULATION-STRUCTURE; GENOTYPIC VARIATION; M.-SACCHARIFLORUS; SINENSIS POACEAE; COLD TOLERANCE; R PACKAGE; MARKERS; DIVERSITY; GRASS AB Background and aims Miscanthus is a genus of perennial C-4 grasses native to East Asia. It includes the emerging ligno-cellulosic biomass crop M. x giganteus, a hybrid between M. sinensis and M. sacchariflorus. Biomass yield and cold tolerance are of particular interest in Miscanthus, given that this crop is more temperate adapted than its C4 relatives maize, sorghum and sugarcane. Methods A plant exploration was conducted in eastern Russia, at the northern extreme of the native range for Miscanthus, with collections including 174 clonal germplasm accessions (160 M. sacchariflorus and 14 M. sinensis) from 47 sites. Accessions were genotyped by restriction site-associated DNA sequencing (RAD-seq) and plastid microsatellites. Key Results Miscanthus sinensis was found in maritime climates near Vladivostok (43.6 degrees N) and on southern Sakhalin Island (46.6 degrees N). Miscanthus sacchariflorus was found inland at latitudes as high as 49.3 degrees N, where M. sinensis was absent. Most M. sacchariflorus accessions were diploid, but approx. 2% were tetraploids. Molecular markers revealed little population structure (Jost's D < 0.007 among diploid groups) but high genetic diversity (expected heterozygosity = 0.14) within the collection of Russian M. sacchariflorus. Genome-wide association (GWA) analysis for traits measured at the collection sites revealed three M. sacchariflorus single nucleotide polymorphisms (SNPs) significantly associated with the number of stems per unit area, one with height and one with basal stem diameter; three were near or within previously described sorghum quantitative trait loci for related traits. Conclusions This new Miscanthus germplasm collection from eastern Russia will be useful for breeding Miscanthus and sugarcane cultivars with improved adaptation to cold. Moreover, a strategy is proposed to facilitate the rapid utilization of new germplasm collections: by implementing low-cost SNP genotyping to conduct GWA studies of phenotypic data obtained at collection sites, plant breeders can be provided with actionable information on which accessions have desirable traits and alleles. C1 [Clark, Lindsay V.; Sacks, Erik J.] Univ Illinois, Dept Crop Sci, 1201 W Gregory Dr, Urbana, IL 61802 USA. [Dzyubenko, Elena; Dzyubenko, Nikolay; Bagmet, Larisa; Sabitov, Andrey; Chebukin, Pavel] Vavilov All Russian Inst Plant Genet Resources, 42-44 Bolshaya Morskaya St, St Petersburg 190000, Russia. [Johnson, Douglas A.] Utah State Univ, USDA ARS Forage & Range Res Lab, Logan, UT 84322 USA. [Kjeldsen, Jens Bonderup; Jorgensen, Uffe] Aarhus Univ, Dept Agroecol, Blichers Alle 20, DK-8830 Tjele, Denmark. [Petersen, Karen Koefoed] Aarhus Univ, Dept Food Sci, Kirstinebjergvej 10, DK-5792 Arslev, Denmark. [Yoo, Ji Hye; Heo, Kweon; Yu, Chang Yeon] Kangwon Natl Univ, Chunchon 200701, Gangwon, South Korea. [Zhao, Hua] Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China. [Jin, Xiaoli] Zhejiang Univ, Dept Agron, Key Lab Crop Germplasm Resource Zhejiang Prov, Hangzhou 310058, Zhejiang, Peoples R China. [Peng, Junhua] China Seed Grp Co Ltd, Ctr Sci & Technol, Wuhan 430040, Hubei, Peoples R China. [Yamada, Toshihiko] Hokkaido Univ, Field Sci Ctr Northern Biosphere, Sapporo, Hokkaido 0600810, Japan. RP Sacks, EJ (reprint author), Univ Illinois, Dept Crop Sci, 1201 W Gregory Dr, Urbana, IL 61802 USA. EM esacks@illinois.edu RI Yamada, Toshihiko/A-5111-2012 OI Yamada, Toshihiko/0000-0002-7845-6556 FU USDA-ARS NPGS Plant Exploration Program [59-1275-1-338]; DOE Office of Science, Office of Biological and Environmental Research (BER) [DE-SC0006634, DE-SC0012379] FX We gratefully acknowledge Inga Kotlyarskaya and Andrey Kseudz for their expert guiding and driving during the collection expedition, and Melina Salgado for assisting with DNA extraction and PCR. Karen Williams, Melanie Harrison, the editor Susanne Barth and two anonymous reviewers provided valuable feedback on earlier drafts of this manuscript. This work was supported by the USDA-ARS NPGS Plant Exploration Program [Cooperative Agreement #59-1275-1-338] and the DOE Office of Science, Office of Biological and Environmental Research (BER) [grant nos DE-SC0006634 and DE-SC0012379]. NR 76 TC 1 Z9 1 U1 9 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7364 EI 1095-8290 J9 ANN BOT-LONDON JI Ann. Bot. PD OCT PY 2016 VL 118 IS 5 BP 941 EP 955 DI 10.1093/aob/mcw137 PG 15 WC Plant Sciences SC Plant Sciences GA EA3GJ UT WOS:000386489600006 ER PT J AU Curzon, MT D'Amato, AW Palik, BJ AF Curzon, Miranda T. D'Amato, Anthony W. Palik, Brian J. TI Bioenergy harvest impacts to biodiversity and resilience vary across aspen-dominated forest ecosystems in the Lake States region, USA SO APPLIED VEGETATION SCIENCE LA English DT Article DE Aspen; Biodiversity; Bioenergy harvest; Community composition; Disturbance; Functional diversity; Populus tremuloides; Recovery; Resilience ID LAND-USE INTENSIFICATION; TERM SOIL PRODUCTIVITY; FUNCTIONAL DIVERSITY; SPECIES RICHNESS; RESPONSE DIVERSITY; CLIMATE-CHANGE; DISTURBANCE; TRAITS; AVAILABILITY; FRAMEWORK AB QuestionsDoes the increase in disturbance associated with removing harvest residues negatively impact biodiversity and resilience in aspen-dominated forest ecosystems? How do responses of functional diversity measures relate to community recovery and standing biomass? LocationAspen (Populus tremuloides, Michx.) mixedwood forests in Minnesota and Michigan, USA. MethodsThree levels for two factors, organic matter removal and compaction, were fully crossed, resulting in nine experimental treatments that spanned a range of disturbance severity. Each treatment was replicated three times at each of three sites dominated by the same tree species but having different soil textures (clay, silty loam, sandy). Community composition and taxonomic diversity (species richness, species evenness, Shannon diversity index) were quantified based on woody species abundance sampled 5, 10 and 15yr after disturbance. Community composition response was assessed using non-metric multidimensional scaling. Functional diversity (functional richness, evenness, dispersion and divergence) was also estimated using eight species effect and response traits. Finally, we examined community recovery as well as responses of species and functional diversity to disturbance severity over time using repeated measures ANOVA. ResultsTwo responses indicated a potentially negative impact of whole-tree harvest relative to conventional, stem-only harvest: functional richness on silty loam soils and species evenness on clayey soils. Otherwise, negative impacts were restricted to forest floor removal or increased compaction. Recovery in community composition was reduced by the most severe treatments, particularly forest floor removal, across the study, but the responses of functional and taxonomic diversity varied among sites, with some measures increasing as a result of severe disturbance. ConclusionsMaximization of standing biomass may mean a short-term sacrifice in species and functional diversity. Also, examinations of forest management impacts on species and functional diversity and composition should apply multiple metrics and indices to ensure potential impacts are not obscured by the reliance on a single approach. C1 [Curzon, Miranda T.; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, 1530 Cleveland Ave North, St Paul, MN 55108 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, 81 Carrigan Dr, Burlington, VT 05405 USA. [Palik, Brian J.] US Forest Serv, USDA, Northern Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. RP Curzon, MT (reprint author), Univ Minnesota, Dept Forest Resources, 1530 Cleveland Ave North, St Paul, MN 55108 USA. EM mcurzon@umn.edu; awdamato@uvm.edu; bpalik@fs.fed.us OI Curzon, Miranda /0000-0002-4102-7352 FU DOE/USDA Biomass Research and Development Initiative; USDA Forest Service; Northern Research Station; Doctoral Dissertation Fellowship from the University of Minnesota FX The authors thank J. Elioff, D. Kastendick, J. Kragthorpe and many other USDA Forest Service scientists and technicians for their work in starting and maintaining the LTSP study. M. Carson, F. Falzone and S. Graves provided technical assistance. S. Fraver and M. Cornett provided helpful comments. D. Waller and G. Sonnier shared species trait data. Funding was provided by the DOE/USDA Biomass Research and Development Initiative, the USDA Forest Service, Northern Research Station, and a Doctoral Dissertation Fellowship from the University of Minnesota to M. Curzon. NR 55 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD OCT PY 2016 VL 19 IS 4 BP 667 EP 678 DI 10.1111/avsc.12256 PG 12 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DZ8QT UT WOS:000386136600013 ER PT J AU Golay, MG Thompson, J Kolka, R AF Golay, Michaeleen Gerken Thompson, Janette Kolka, Randall TI Carbon, nitrogen and phosphorus storage across a growing season by the herbaceous layer in urban and preserved temperate hardwood forests SO APPLIED VEGETATION SCIENCE LA English DT Article DE Autumn; Ecosystem services; Forest understorey community composition; Herbaceous biomass; Nutrient storage; Soil nutrients; Urban forest ID DECIDUOUS-FOREST; ERYTHRONIUM-AMERICANUM; UNDERSTORY VEGETATION; NUTRIENT RESORPTION; TERRESTRIAL PLANTS; SOIL; ECOSYSTEMS; DYNAMICS; DIVERSITY; PHENOLOGY AB QuestionHerbaceous plant communities in hardwood forests are important for maintaining biodiversity and associated ecosystem services, such as nutrient storage. Are there differences in herbaceous layer nutrient storage for urban park and state preserve forests, and is there seasonal variation? LocationUnited States, Midwestern region (Iowa). MethodsWe examined the herbaceous layer in three 20-m(2) plots at six forest sites, in urban parks (city parks with high human visitation) and state preserves (under permanent protection as state preserves). We harvested herbaceous plants from quadrats in each plot in spring, summer and autumn to analyse above- and below-ground tissues for total C, N and P concentrations. Biomass and tissue nutrient concentration data were used to estimate nutrient storage per plot and per hectare. We also collected soil samples at each plot in each season to determine soil C, N and P content. ResultsState preserve and urban park forests did not differ in herbaceous species richness, although state preserve forests were qualitatively distinct. State preserve forests had relatively higher biomass and nutrients than urban park forests. In both forest types, above-ground concentration for N and P were higher in spring than in autumn, whereas below-ground concentration of these nutrients was consistently higher in autumn. In urban parks, total soil N was higher in spring compared to summer and autumn. However, soil nutrient content did not appear to drive differences in plant tissue nutrient content in urban park forests. ConclusionsSubtle qualitative differences in herbaceous layer composition affected seasonal biomass quantities and nutrient concentrations in urban park and state preserve forests. These differences influenced C, N and P storage and led to consistent trends for relatively higher biomass and nutrient storage in state preserve forests. Above-ground plant tissue provides important storage of N and P in spring, and below-ground plant tissue provides important storage of N and P in autumn. Since spring and autumn are seasons of limited vegetative cover in the regional landscape, with subsequently higher potential for nutrient loss from terrestrial systems, this finding is crucial for provision of ecosystem services. C1 [Golay, Michaeleen Gerken] Wartburg Coll, Dept Biol, 100 Wartburg Blvd, Waverly, IA 50677 USA. [Thompson, Janette] Iowa State Univ, Dept Nat Resource Ecol & Management, Sci 2, Ames, IA 50011 USA. [Kolka, Randall] US Forest Serv, Northern Res Stn, 1831 Hwy 169 East, Grand Rapids, MN 55744 USA. RP Golay, MG (reprint author), Wartburg Coll, Dept Biol, 100 Wartburg Blvd, Waverly, IA 50677 USA. EM michaeleen.golay@wartburg.edu; jrrt@iastate.edu; rkolka@fs.fed.us FU US Forest Service Northern Research Station; Leopold Center for Sustainable Agriculture; McIntire-Stennis funding; Iowa State University Department of Natural Resource Ecology and Management FX This study was funded by the US Forest Service Northern Research Station and the Leopold Center for Sustainable Agriculture, with additional support from McIntire-Stennis funding and the Iowa State University Department of Natural Resource Ecology and Management. We thank departmental graduate assistants Jiayu Wu, Alister Olson, Zachary Keninger and Em Kapler, in addition to undergraduate assistants Joe Bolton, Justin Landhuis, Greg Martin and Rob Manatt for help with field data collection. We also thank John Larson, USFS Northern Research Station, for assistance with laboratory analyses and Sr.Nancy Kinate, for editorial review. NR 49 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD OCT PY 2016 VL 19 IS 4 BP 689 EP 699 DI 10.1111/avsc.12253 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DZ8QT UT WOS:000386136600015 ER PT J AU Bell, KC Calhoun, KL Hoberg, EP Demboski, JR Cook, JA AF Bell, Kayce C. Calhoun, Kendall L. Hoberg, Eric P. Demboski, John R. Cook, Joseph A. TI Temporal and spatial mosaics: deep host association and shallow geographic drivers shape genetic structure in a widespread pinworm, Rauschtineria eutamii (Nematoda: Oxyuridae) SO BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Article DE chipmunk; ecological fitting; parasite; phylogeography; Tamias; taxon pulse; western North America ID NORTH-AMERICAN CHIPMUNKS; CLIMATE-CHANGE; HISTORICAL BIOGEOGRAPHY; METAZOAN PARASITES; DICROSTONYX-SPP.; TAMIAS; PHYLOGEOGRAPHY; DIVERSIFICATION; PHYLOGENY; ANOPLOCEPHALIDAE AB Climate and host demographic cycling often shape both parasite genetic diversity and host distributions, processes that transcend a history of strict host-parasite association. We explored host associations and histories based on an evaluation of mitochondrial and nuclear sequences to reveal the underlying history and genetic structure of a pinworm, Rauschtineria eutamii, infecting ten species of western North American chipmunks (Rodentia: Tamias, subgenus Neotamias). Rauschtineria eutamii contains divergent lineages influenced by the diversity of hosts and variation across the complex topography of western North America. We recovered six reciprocally monophyletic R. eutamii mitochondrial clades, largely supported by a multilocus concordance tree, exhibiting divergence levels comparable with intraspecific variation reported for other nematodes. Phylogenetic relationships among pinworm clades suggest that R. eutamii colonized an ancestral lineage of western chipmunks and lineages persisted during historical isolation in diverging Neotamias species or species groups. Pinworm diversification, however, is incongruent and asynchronous relative to host diversification. Secondarily, patterns of shallow divergence were shaped by geography through events of episodic colonization reflecting an interaction of taxon pulses and ecological fitting among assemblages in recurrent sympatry. Pinworms occasionally infect geographically proximal host species; however, host switching may be unstable or ephemeral, as there is no signal of host switching in the deeper history of R. eutamii. (c) 2016 The Linnean Society of London, Biological Journal of the Linnean Society, 2016, 119, 397-413. C1 [Bell, Kayce C.; Cook, Joseph A.] Univ New Mexico, Dept Biol, Museum Southwestern Biol, Albuquerque, NM 87108 USA. [Calhoun, Kendall L.] Univ Calif Berkeley, Environm Sci Policy & Management, Berkeley, CA USA. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, USDA, Beltsville Area Res Ctr, BARC East 1180,10300 Baltimore Ave, Beltsville, MD 20715 USA. [Demboski, John R.] Denver Museum Nat & Sci, Dept Zool, 2001 Colorado Blvd, Denver, CO 80205 USA. RP Bell, KC (reprint author), Univ New Mexico, Dept Biol, Museum Southwestern Biol, Albuquerque, NM 87108 USA. EM kbell01@unm.edu FU Department of Biology at UNM; Biology Graduate Student Association at UNM; American Society of Parasitologists; American Society of Mammalogists; Society of Systematic Biologists; Lloyd David and Carlye Cannon Wattis Foundation; National Science Foundation [DEB-0716200, 0956129, 1258010, 1311076]; University of California, Berkeley [NIH NIGMS T34GM092702] FX Three anonymous reviewers provided valuable input and helpful comments. We thank J. L. Patton, D. S. Rogers, E. A. Rickart, J. Bradley, and A. D. Hornsby for contributing samples. B. S. McLean, R. McCain, S. W. Liphardt, L. F. Alexander, I. A. Demboski, W. Bell, J. Campbell, and P. McDonald assisted with fieldwork and A. Hope helped with analyses. The Department of Biology and Biology Graduate Student Association at UNM, the American Society of Parasitologists, the American Society of Mammalogists, the Society of Systematic Biologists, the Lloyd David and Carlye Cannon Wattis Foundation, and National Science Foundation (DEB-0716200, 0956129, 1258010, 1311076) funded aspects of this research. The Maximizing Access to Research Careers Program at University of California, Berkeley (NIH NIGMS T34GM092702) funded K. Calhoun's time to work on this project. State wildlife agencies granted permits for specimen collection. NR 88 TC 1 Z9 1 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4066 EI 1095-8312 J9 BIOL J LINN SOC JI Biol. J. Linnean Soc. PD OCT PY 2016 VL 119 IS 2 BP 397 EP 413 DI 10.1111/bij.12833 PG 17 WC Evolutionary Biology SC Evolutionary Biology GA EA8VY UT WOS:000386919000011 PM 27725785 ER PT J AU Musavi, T Migliavacca, M van de Weg, MJ Kattge, J Wohlfahrt, G van Bodegom, PM Reichstein, M Bahn, M Carrara, A Domingues, TF Gavazzi, M Gianelle, D Gimeno, C Granier, A Gruening, C Havrankova, K Herbst, M Hrynkiw, C Kalhori, A Kaminski, T Klumpp, K Kolari, P Longdoz, B Minerbi, S Montagnani, L Moors, E Oechel, WC Reich, PB Rohatyn, S Rossi, A Rotenberg, E Varlagin, A Wilkinson, M Wirth, C Mahecha, MD AF Musavi, Talie Migliavacca, Mirco van de Weg, Martine Janet Kattge, Jens Wohlfahrt, Georg van Bodegom, Peter M. Reichstein, Markus Bahn, Michael Carrara, Arnaud Domingues, Tomas F. Gavazzi, Michael Gianelle, Damiano Gimeno, Cristina Granier, Andre Gruening, Carsten Havrankova, Katerina Herbst, Mathias Hrynkiw, Charmaine Kalhori, Aram Kaminski, Thomas Klumpp, Katja Kolari, Pasi Longdoz, Bernard Minerbi, Stefano Montagnani, Leonardo Moors, Eddy Oechel, Walter C. Reich, Peter B. Rohatyn, Shani Rossi, Alessandra Rotenberg, Eyal Varlagin, Andrej Wilkinson, Matthew Wirth, Christian Mahecha, Miguel D. TI Potential and limitations of inferring ecosystem photosynthetic capacity from leaf functional traits SO ECOLOGY AND EVOLUTION LA English DT Article DE ecosystem functional property; eddy covariance; FLUXNET; interannual variability; photosynthetic capacity; plant traits; spatiotemporal variability; TRY database ID GROSS PRIMARY PRODUCTION; CROSS-BIOME ANALYSIS; RELATIVE GROWTH-RATE; PLANT TRAITS; CARBON-DIOXIDE; FOREST PRODUCTIVITY; WIDE-RANGE; ENVIRONMENTAL VARIATION; NUTRIENT CONCENTRATIONS; TERRESTRIAL BIOSPHERE AB The aim of this study was to systematically analyze the potential and limitations of using plant functional trait observations from global databases versus in situ data to improve our understanding of vegetation impacts on ecosystem functional properties (EFPs). Using ecosystem photosynthetic capacity as an example, we first provide an objective approach to derive robust EFP estimates from gross primary productivity (GPP) obtained from eddy covariance flux measurements. Second, we investigate the impact of synchronizing EFPs and plant functional traits in time and space to evaluate their relationships, and the extent to which we can benefit from global plant trait databases to explain the variability of ecosystem photosynthetic capacity. Finally, we identify a set of plant functional traits controlling ecosystem photosynthetic capacity at selected sites. Suitable estimates of the ecosystem photosynthetic capacity can be derived from light response curve of GPP responding to radiation (photosynthetically active radiation or absorbed photosynthetically active radiation). Although the effect of climate is minimized in these calculations, the estimates indicate substantial interannual variation of the photosynthetic capacity, even after removing site-years with confounding factors like disturbance such as fire events. The relationships between foliar nitrogen concentration and ecosystem photosynthetic capacity are tighter when both of the measurements are synchronized in space and time. When using multiple plant traits simultaneously as predictors for ecosystem photosynthetic capacity variation, the combination of leaf carbon to nitrogen ratio with leaf phosphorus content explains the variance of ecosystem photosynthetic capacity best (adjusted R-2 = 0.55). Overall, this study provides an objective approach to identify links between leaf level traits and canopy level processes and highlights the relevance of the dynamic nature of ecosystems. Synchronizing measurements of eddy covariance fluxes and plant traits in time and space is shown to be highly relevant to better understand the importance of intra-and interspecific trait variation on ecosystem functioning. C1 [Musavi, Talie; Migliavacca, Mirco; Kattge, Jens; Reichstein, Markus; Wirth, Christian; Mahecha, Miguel D.] Max Planck Inst Biogeochem, Jena, Germany. [van de Weg, Martine Janet] Vrije Univ Amsterdam, Amsterdam Global Change Inst, Amsterdam, Netherlands. [Kattge, Jens; Reichstein, Markus; Wirth, Christian; Mahecha, Miguel D.] German Ctr Integrat Biodivers Res IDiv, Leipzig, Germany. [Wohlfahrt, Georg; Bahn, Michael] Univ Innsbruck, Inst Ecol, Innsbruck, Austria. [van Bodegom, Peter M.] Leiden Univ, Inst Environm Sci, Leiden, Netherlands. [Carrara, Arnaud; Gimeno, Cristina] Fdn CEAM, Mediterranean Ctr Environm Studies, Valencia, Spain. [Domingues, Tomas F.] FFCLRP USP, Ribeirao Preto, Brazil. [Gavazzi, Michael] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC USA. [Gianelle, Damiano] Fdn Edmund Mach, Res & Innovat Ctr, Dept Sustainable Agroecosyst & Bioresources, Trento, Italy. [Gianelle, Damiano] Foxlab Joint CNR FEM Initiat, Trento, Italy. [Granier, Andre; Longdoz, Bernard] INRA, UMR Ecol & Ecophysiol Forestieres 1137, Champenoux, France. [Gruening, Carsten] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, Ispra, Italy. [Havrankova, Katerina] Global Change Res Inst CAS, Dept Matters & Energy Fluxes, Brno, Czech Republic. [Herbst, Mathias] Fed Res Inst Rural Areas Forestry & Fisheries, Johann Heinrich von Thunen Inst, Braunschweig, Germany. [Hrynkiw, Charmaine] Natl Hydrol Res Ctr NHRC, Saskatoon, SK, Canada. [Kalhori, Aram; Oechel, Walter C.; Rossi, Alessandra] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Kaminski, Thomas] Invers Lab, Hamburg, Germany. [Klumpp, Katja] INRA, Grassland Ecosyst Res UR874, Clermont Ferrand, France. [Kolari, Pasi] Univ Helsinki, Dept Phys, Helsinki, Finland. [Minerbi, Stefano; Montagnani, Leonardo] Prov Autonoma Bolzano Serv Forestali, Bolzano, Italy. [Montagnani, Leonardo] Free Univ Bolzano, Fac Sci & Technol, Bolzano, Italy. [Moors, Eddy] Alterra Green World Res, Wageningen, Netherlands. [Oechel, Walter C.] Open Univ, Dept Environm Earth & Ecosyst, Milton Keynes, Bucks, England. [Reich, Peter B.] Univ Minnesota Twin Cities, Dept Forest Resources, St Paul, MN USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW, Australia. [Rohatyn, Shani] Hebrew Univ Jerusalem, Fac Agr Food & Environm Qual Sci, Soil & Water Dept, Rehovot, Israel. [Rohatyn, Shani; Rotenberg, Eyal] Weizmann Inst Sci, Dept Earth & Planetary Sci, Rehovot, Israel. [Varlagin, Andrej] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow, Russia. [Wilkinson, Matthew] Alice Holt Lodge, Forest Res, Environm & Human Sci Div, Farnham, Surrey, England. [Wirth, Christian] Univ Leipzig, Inst Special Bot & Funct Biodivers, Leipzig, Germany. RP Musavi, T (reprint author), Max Planck Inst Biogeochem, Jena, Germany. EM tmusavi@bgc-jena.mpg.de RI Gianelle, Damiano/G-9437-2011; Wohlfahrt, Georg/D-2409-2009; OI Gianelle, Damiano/0000-0001-7697-5793; Wohlfahrt, Georg/0000-0003-3080-6702; Kolari, Pasi/0000-0001-7271-633X; Montagnani, Leonardo/0000-0003-2957-9071 FU European Union [640176] FX European Union's Horizon 2020 project BACI under grant agreement No. 640176. NR 131 TC 0 Z9 0 U1 25 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD OCT PY 2016 VL 6 IS 20 BP 7352 EP 7366 DI 10.1002/ece3.2479 PG 15 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA EA2NA UT WOS:000386429200018 ER PT J AU Nevill, PG Tomlinson, S Elliott, CP Espeland, EK Dixon, KW Merritt, DJ AF Nevill, Paul G. Tomlinson, Sean Elliott, Carole P. Espeland, Erin K. Dixon, Kingsley W. Merritt, David J. TI Seed production areas for the global restoration challenge SO ECOLOGY AND EVOLUTION LA English DT Editorial Material ID ECOLOGICAL RESTORATION; GREAT-BASIN; MANAGEMENT IMPLICATIONS; PLANT MATERIALS; AUSTRALIA; POLLINATION; SYSTEMS; FRAMEWORK; MANGROVES; PATTERNS C1 [Nevill, Paul G.; Tomlinson, Sean; Elliott, Carole P.; Dixon, Kingsley W.; Merritt, David J.] Kings Pk & Bot Gardens, Kings Pk, WA, Australia. [Nevill, Paul G.; Dixon, Kingsley W.; Merritt, David J.] Univ Western Australia, Sch Plant Biol, Nedlands, WA, Australia. [Elliott, Carole P.] Murdoch Univ, Sch Vet & Life Sci Environm & Conservat Sci, Murdoch, WA, Australia. [Espeland, Erin K.] USDA ARS, Pest Management Res Unit, Sidney, MT USA. [Nevill, Paul G.; Dixon, Kingsley W.] Curtin Univ, ARC Ctr Mine Restorat, Dept Environm & Agr, Bentley, WA 6102, Australia. RP Nevill, PG (reprint author), Curtin Univ, Dept Environm & Agr, Perth, WA, Australia. EM paul.nevill@curtin.edu.au NR 71 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD OCT PY 2016 VL 6 IS 20 BP 7490 EP 7497 DI 10.1002/ece3.2455 PG 8 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA EA2NA UT WOS:000386429200030 ER PT J AU Smith, JB Turner, KL Beasley, JC DeVault, TL Pitt, WC Rhodes, OE AF Smith, Joshua B. Turner, Kelsey L. Beasley, James C. DeVault, Travis L. Pitt, William C. Rhodes, Olin E., Jr. TI Brown tree snake (Boiga irregularis) population density and carcass locations following exposure to acetaminophen SO ECOTOXICOLOGY LA English DT Article DE Acetaminophen; Carcass location; Brown tree snake; Indirect toxicant transfer; Scavenging ID RESOURCE PULSES; GUAM; ISLAND; TREESNAKES; CARRION; DECOMPOSITION; SCAVENGERS; REPTILIA; EFFICACY; IMPACTS AB Mass aerial delivery of dead mouse baits treated with acetaminophen has been evaluated as a means to reduce brown tree snake (Boiga irregularis) populations over large areas, increasing the likelihood of wide-scale eradication on Guam. Given the high density of snakes in some areas of their invasive range, eradication efforts could result in a resource pulse that may influence food web dynamics and the indirect transport of acetaminophen among trophic levels. We evaluated abundance, habitat type, and snake size (i.e., age) within two study sites on Guam, a secondary limestone forest (upland) and an abandoned coconut plantation (coastal), to determine how experimentally dosing snakes with acetaminophen is likely to influence carrion availability. We found snakes trapped in 3.24 ha plots occurred in greater abundance (population size = 72.5 snakes; SE = 8.8) and were significantly larger (978.6 mm, SE = 14.9) in the coastal than in the upland site (population size = 26.9, SE = 21.5; length = 903.0 mm, SE = 15.9). Despite these differences, carcasses of snakes that died after consuming acetaminophen-laced mice (80 mg) were recovered in consistent locations between sites, with 92 % located on the ground, 4 % in trees, and 4 % found in rock cavities at both sites. Given that most snakes were found on the ground rather than in the tree canopy, our results suggest that many poisoned snake carcasses will be accessible to a wide range of potential scavengers, possibly influencing food web dynamics and potentially contributing to indirect toxicant transfer within affected ecosystems. C1 [Smith, Joshua B.; Turner, Kelsey L.; Beasley, James C.; Rhodes, Olin E., Jr.] Savannah River Ecol Lab, PO Drawer E, Aiken, SC 29802 USA. [Turner, Kelsey L.; Beasley, James C.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [DeVault, Travis L.] USDA, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH USA. [Pitt, William C.] Smithsonian Conservat Biol Inst, 1500 Remount Rd, Front Royal, VA 22630 USA. [Rhodes, Olin E., Jr.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. RP Smith, JB (reprint author), Savannah River Ecol Lab, PO Drawer E, Aiken, SC 29802 USA. EM jsmith77@uga.edu FU University of Georgia Research Foundation; United Stated Department of the Navy via the United States Department of Agriculture National Wildlife Research Center [14-7439-1099-CA]; United States Department of Energy [DE-FC09-07SR22506] FX We thank M. Hall, S. Mosher, D. Lujan, and the staff at the United States Department of Agriculture Wildlife Service Andersen Air Force Base, Guam for logistical support and capture assistance. This work was supported through Cooperative Agreements between the University of Georgia Research Foundation and the United Stated Department of the Navy via the United States Department of Agriculture National Wildlife Research Center (No. 14-7439-1099-CA) and the United States Department of Energy (No. DE-FC09-07SR22506). NR 51 TC 0 Z9 0 U1 10 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0963-9292 EI 1573-3017 J9 ECOTOXICOLOGY JI Ecotoxicology PD OCT PY 2016 VL 25 IS 8 BP 1556 EP 1562 DI 10.1007/s10646-016-1711-1 PG 7 WC Ecology; Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA EA6BU UT WOS:000386710800010 PM 27604786 ER PT J AU Baranowski, T AF Baranowski, Tom TI Pokemon Go, go, go, gone? SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material ID CHILDHOOD OBESITY; PHYSICAL-ACTIVITY; VIDEO GAMES C1 [Baranowski, Tom] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Coll Med, Pediat Behav Nutr & Phys Act, Houston, TX 77030 USA. EM Thomas.Baranowski@bcm.edu NR 17 TC 0 Z9 0 U1 19 U2 19 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD OCT PY 2016 VL 5 IS 5 BP 293 EP 294 DI 10.1089/g4h.2016.01055.tbp PG 2 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA EA8AS UT WOS:000386856900001 ER PT J AU Thompson, D Cantu, D Rajendran, M Rajendran, M Bhargava, T Zhang, YT Chen, C Liu, Y Deng, ZG AF Thompson, Debbe Cantu, Dora Rajendran, Madhur Rajendran, Mayur Bhargava, Tanay Zhang, Yuting Chen, Cheng Liu, Yan Deng, Zhigang TI Development of a Teen-Focused Exergame SO GAMES FOR HEALTH JOURNAL LA English DT Article DE Physical activity; Virtual world; Exergame; Avatars; Self Determination Theory; Adolescents ID PHYSICAL-ACTIVITY; VIDEO GAMES; ENERGY-EXPENDITURE; BEHAVIOR-CHANGE; CHILDREN; SELF; HEALTH; OBESITY; WEIGHT; YOUTH AB Introduction: Exergames require body movement to play and may be an effective method for enhancing teen physical activity (PA). However, results have been mixed. Innovative methods are needed to develop Exergames that increase and maintain PA. Self-representational avatars, or avatars created from a digital image of an individual, may increase PA (e.g., intensity, duration) during Exergame play. This article addresses this novel idea by describing the development of an Exergame played with a self-representational avatar. Materials and Methods: Twelve- to 14-year-olds, stratified by gender, body mass index, and PA, were invited to participate in two rounds of data collection. Each round consisted of an online survey, followed by a telephone interview to ensure comprehension of survey responses. After the first round, an Exergame prototype and the system for creating the self-representational avatar were created. A second round of data was collected to obtain information with which to create a fully functional Exergame and the avatar creation system. Results: Forty-eight teens were recruited. The sample was multi-ethnic (41.7% White, 37.5% Black, 18.8% Hispanic, 2.1% Mixed/Other). Complete data were obtained on 48 teens in the first round of data collection and on 43 teens in the second round. Teens provided important information regarding preferences and expectations. Gender similarities and differences were observed. Conclusion: This research contributes to the body of knowledge regarding how to design an appealing Exergame for teens navigated by a self-representational avatar. C1 [Thompson, Debbe; Cantu, Dora; Liu, Yan] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Rajendran, Madhur; Rajendran, Mayur; Bhargava, Tanay; Zhang, Yuting; Chen, Cheng; Deng, Zhigang] Univ Houston, Dept Comp Sci, 4800 Calhoun Rd, Houston, TX 77204 USA. RP Deng, ZG (reprint author), Univ Houston, Dept Comp Sci, 4800 Calhoun Rd, Houston, TX 77204 USA. EM zdeng4@uh.edu NR 39 TC 0 Z9 0 U1 3 U2 3 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD OCT PY 2016 VL 5 IS 5 BP 342 EP 356 DI 10.1089/g4h.2016.0035 PG 15 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA EA8AS UT WOS:000386856900008 ER PT J AU Moisen, GG Meyer, MC Schroeder, TA Liao, XY Schleeweis, KG Freeman, EA Toney, C AF Moisen, Gretchen G. Meyer, Mary C. Schroeder, Todd A. Liao, Xiyue Schleeweis, Karen G. Freeman, Elizabeth A. Toney, Chris TI Shape selection in Landsat time series: a tool for monitoring forest dynamics SO GLOBAL CHANGE BIOLOGY LA English DT Article DE attribution; canopy change activities; change agents; forest disturbance; landcover change; R package; regression splines; tree canopy cover ID CONSTRAINED REGRESSION; DISTURBANCE HISTORY; DETECTING TRENDS; COVER; IMAGERY AB We present a new methodology for fitting nonparametric shape-restricted regression splines to time series of Landsat imagery for the purpose of modeling, mapping, and monitoring annual forest disturbance dynamics over nearly three decades. For each pixel and spectral band or index of choice in temporal Landsat data, our method delivers a smoothed rendition of the trajectory constrained to behave in an ecologically sensible manner, reflecting one of seven possible 'shapes'. It also provides parameters summarizing the patterns of each change including year of onset, duration, magnitude, and pre- and postchange rates of growth or recovery. Through a case study featuring fire, harvest, and bark beetle outbreak, we illustrate how resultant fitted values and parameters can be fed into empirical models to map disturbance causal agent and tree canopy cover changes coincident with disturbance events through time. We provide our code in the R package ShapeSelectForest on the Comprehensive R Archival Network and describe our computational approaches for running the method over large geographic areas. We also discuss how this methodology is currently being used for forest disturbance and attribute mapping across the conterminous United States. C1 [Moisen, Gretchen G.; Schroeder, Todd A.; Schleeweis, Karen G.; Freeman, Elizabeth A.; Toney, Chris] US Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. [Meyer, Mary C.; Liao, Xiyue] Colorado State Univ, Dept Stat, 212 Stat Bldg, Ft Collins, CO 80523 USA. [Schroeder, Todd A.] US Geol Survey, ASRC Fed InuTeq, Earth Resources Observat & Sci Ctr, Sioux Falls, SD 57198 USA. RP Moisen, GG (reprint author), US Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM gmoisen@fs.fed.us FU NASA's Carbon Cycle and Applied Science programs [NNX11AJ78G, NNH14AY63I]; US Forest Service, FIA Program FX The authors would like to thank NASA's Carbon Cycle and Applied Science programs (grant numbers NNX11AJ78G and NNH14AY63I) as well as the very talented scientists and staff at NASA's Earth Exchange, University of Maryland, and the US Forest Service, FIA Program. Thanks also go out to the members of the LCMS team for their efforts pushing change mapping forward in the US. In addition, we are grateful to the anonymous reviewers whose thoughtful comments helped in our revisions. NR 40 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD OCT PY 2016 VL 22 IS 10 BP 3518 EP 3528 DI 10.1111/gcb.13358 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA EA5RQ UT WOS:000386680600023 PM 27185612 ER PT J AU Ralston, J DeLuca, WV Feldman, RE King, DI AF Ralston, Joel DeLuca, William V. Feldman, Richard E. King, David I. TI Realized climate niche breadth varies with population trend and distribution in North American birds SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE Abundance; Breeding Bird Survey; Grinnellian niche; Maxent; species distribution ID ENVELOPE MODELS; RANGE SIZE; SPECIES DISTRIBUTIONS; MIGRATORY SONGBIRD; HABITAT SELECTION; ABUNDANCE; DECLINES; MAXENT; PERFORMANCE; SOFTWARE AB AimEcological niche theory states that realized niche breadth should increase with population growth. This relationship has been studied extensively in the context of density-dependent habitat selection, and there is evidence that animal populations at higher density occupy a wider range of vegetation types. To our knowledge, no previous studies have investigated the relationship between population growth and climate niche breadth (i.e. the range of climatic conditions occupied). Here we aim to estimate the influence of population trend, as well as changes in distribution, on realized climate niche breadth. LocationNorth America. MethodsWe estimated changes in realized climate niche breadth and distribution between 1980 and 2012 for 46 bird species using data from the North American Breeding Bird Survey (BBS) and standard ecological niche modelling techniques. We analysed changes in niche breadth in relation to population trends and distributional changes from the BBS for these same species. ResultsChanges in realized climate niche breadth were significantly and positively associated with population growth, as reflected by BBS population trends, and with changes in distributional extent. Using variance partitioning, we showed that 44.2% of the variation in change in niche breadth can be explained by population trend, and that roughly half of this was independent of changes in distribution. ConclusionsRealized climate niche breadth is variable on an ecological time-scale as a function of population trend. Mechanisms associated with changes in distribution and those acting within current species range limits appear to be equally important in driving this relationship. Observed changes in niche breadth may violate distribution modelling assumptions of niche conservatism. Studying how population growth influences realized climate niche breadth is therefore important for understanding dynamic species distributions, responses to climate change and our ability to model future species distributions. C1 [Ralston, Joel; DeLuca, William V.; Feldman, Richard E.; King, David I.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Feldman, Richard E.] Ctr Invest Cient Yucatan, Unidad Recursos Nat, Merida 97200, Yucatan, Mexico. [King, David I.] US Forest Serv, Northern Res Stn, Amherst, MA 01003 USA. [Ralston, Joel] St Marys Coll, Dept Biol, Notre Dame, IN 46556 USA. RP Ralston, J (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM jralston@saintmarys.edu FU Department of Interior Northeast Climate Science Center FX Funding for this project was provided by the Department of Interior Northeast Climate Science Center. We thank M. Akresh for guidance on statistical analyses and B. Bradley, K. Nislow, T. S. Sillett and three anonymous referees for comments on an earlier draft. NR 59 TC 1 Z9 1 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD OCT PY 2016 VL 25 IS 10 BP 1173 EP 1180 DI 10.1111/geb.12490 PG 8 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA DW5IP UT WOS:000383677800003 ER PT J AU Kindiger, B AF Kindiger, Bryan TI Generation of paternal dihaploids in tall fescue SO GRASSLAND SCIENCE LA English DT Article DE dihaploid; Lolium; tall fescue ID VIVO HAPLOID INDUCTION; DOUBLED-HAPLOIDS; FESTUCA COMPLEX; LOLIUM; SELECTION; MAIZE; REGISTRATION; POPULATIONS; IMPROVEMENT; HYBRIDS AB Within the Lolium-Festuca genome complex there is a need for modern breeding approaches that can facilitate the rapid development of improved germplasm or cultivars. Traditional recurrent or mass-selection methods for population or synthetic development are labor intensive and time consuming. The recent development of dihaploid (DH) inducer lines of annual ryegrass (Lolium perenne L. subsp. multiflorum (Lam.) Husnot [syn. Lolium multiflorum Lam.]) that exhibit genome loss when hybridized with tall fescue (Lolium arundinaceum (Schreb.) Darbysh. [syn. Festuca arundinacea Schreb.]) can provide an alternative breeding approach. Hybridizations between the inducer lines and tall fescue result in F-1 hybrids that can lose either their ryegrass or tall fescue genomes. This behavior results in the occasional generation of both ryegrass and tall fescue DH lines through parthenogenic embryo formation within the F-1 inflorescence. C1 [Kindiger, Bryan] USDA ARS, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. RP Kindiger, B (reprint author), USDA ARS, Grazinglands Res Lab, 7207 West Cheyenne St, El Reno, OK 73036 USA. EM bryan.kindiger@ars.usda.gov FU USDA-Agricultural Research Service (ARS), Cooperative Research and Development Agreements with Barenbrug Seeds, Albany, OR, USA FX This study was financially supported by a series of USDA-Agricultural Research Service (ARS), Cooperative Research and Development Agreements with Barenbrug Seeds, Albany, OR, USA. The author wishes to thank Devesh Singh and Joseph Wipff for their support and attention toward the implementation of this project. NR 38 TC 1 Z9 1 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1744-6961 EI 1744-697X J9 GRASSL SCI JI Grassl. Sci. PD OCT PY 2016 VL 62 IS 4 BP 243 EP 247 DI 10.1111/grs.12133 PG 5 WC Agriculture, Multidisciplinary; Agronomy SC Agriculture GA EA8XP UT WOS:000386923600005 ER PT J AU Sharma, S Adams, JP Schuler, JL Ficklin, RL Bragg, DC AF Sharma, Shakuntala Adams, Joshua P. Schuler, Jamie L. Ficklin, Robert L. Bragg, Don C. TI Effect of seedling stock on the early stand development and physiology of improved loblolly pine (Pinus taeda L.) seedlings SO IFOREST-BIOGEOSCIENCES AND FORESTRY LA English DT Article DE Loblolly Pine; Genotype; Leaf Water Potential; Coefficient of Variation ID SOUTHERN UNITED-STATES; SUSTAINED PRODUCTIVITY; GROWTH; FORESTS AB This study assessed the effects of spacing and genotype on the growth and physiology of improved loblolly pine (Pinus taeda L.) seedlings from three distinct genotypes planted in Drew County, Arkansas (USA). Genotype had a significant effect on survival and height. Clone CF Var 1 showed greater height and survival compared to other seedlings. Genotype had significant effects on uniformity in height both years and ground line diameter (GLD) first year. However, genotype had no significant effects on leaf water potential and coefficient variation of leaf water potential. These growth and physiology should be further studied to assess potential genetic differences among seedlings and to determine if they can be identified early for improved growth at later ages. C1 [Sharma, Shakuntala] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Adams, Joshua P.] Louisiana Tech Univ, Sch Forest Resources, Ruston, LA 71270 USA. [Schuler, Jamie L.] West Virginia Univ, Morgantown, WV 26506 USA. [Ficklin, Robert L.] Univ Arkansas, Arkansas Forest Resources Ctr, Monticello, AR USA. [Bragg, Don C.] Forest Serv, USDA, Southern Res Stn, Monticello, AR USA. RP Sharma, S (reprint author), Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM shakun109@gmail.com FU Arkansas Forest Resources Center FX We would like to thank Mr. Nicholas Mustoe and Mr. Allan Humphrey for their help in field and lab work. We would also like to thank Arborgen (c) for information on the clones and the Arkansas Forestry Commission and Western Gulf Tree Improvement Cooperative for information on the 3-star half-sibling seedlings developed by the cooperative and grown by the Commission. Research was funded by the Arkansas Forest Resources Center, co-located with the University of Arkansas at Monticello School of Forestry and Natural Resources, Monticello, AR (USA). NR 28 TC 0 Z9 0 U1 4 U2 4 PU SISEF-SOC ITALIANA SELVICOLTURA ECOL FORESTALE PI POTENZA PA DEPT PROD VEGETALE, VIA ATENEO LUCANO 10, POTENZA, 85100, ITALY SN 1971-7458 J9 IFOREST JI iForest PD OCT PY 2016 VL 9 BP 690 EP 695 DI 10.3832/ifor1725-009 PG 6 WC Forestry SC Forestry GA EA3EC UT WOS:000386481500003 ER PT J AU Chow, A Dunlap, CA Jackson, MA Flores, D Patt, JM Setamou, M AF Chow, Andrew Dunlap, Christopher A. Jackson, Mark A. Flores, Daniel Patt, Joseph M. Setamou, Mamoudou TI Oviposition Behavior and Survival of Tamarixia radiata (Hymenoptera: Eulophidae), an Ectoparasitoid of the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae), on Hosts Exposed to an Entomopathogenic Fungus, Isaria fumosorosea (Hypocreales: Cordycipitaceae), Under Laboratory Conditions SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biological control; Diaphorina citri; entomopathogen; Isaria fumosorosea; Tamarixia radiata ID CANDIDATUS LIBERIBACTER ASIATICUS; BASSIANA ASCOMYCOTA HYPOCREALES; CLASSICAL BIOLOGICAL-CONTROL; PARASITOID ENCARSIA-FORMOSA; NATURAL ENEMY BIODIVERSITY; PAECILOMYCES-FUMOSOROSEUS; BEAUVERIA-BASSIANA; VERTICILLIUM-LECANII; ASCHERSONIA-ALEYRODIS; INTRAGUILD PREDATION AB Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 strain of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae), could disrupt biological control of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Three interactions were evaluated under laboratory conditions at 25 degrees C: 1) parasitoid survival if parasitized hosts were exposed to ARSEF 3581 blastospores before or after host mummification; 2) parasitoid survival if mummies containing larva or pupa were exposed to ARSEF 3581 hyphae; 3) parasitoid oviposition on infected hosts with visible or without visible hyphae. Topical application of blastospore formulation onto the dorsal surfaces of live nymphs parasitized with second-instar wasp larva (3 d after parasitism) reduced host mummification by 50% and parasitoid emergence by 85%. However, parasitoid emergence was not affected by topical application of blastospore formulation onto mummies that contained fourth-instar wasp larva (6 d after parasitism). Parasitoid emergence was reduced by 80% if mummies containing fourth-instar wasp larva were covered with blastospore formulation colonized by fungal hyphae. In comparison, parasitoid emergence was not affected if mummies containing wasp pupa (9 d after parasitism) were covered with formulation colonized by fungal hyphae. Female parasitoids oviposited on infected hosts without visible hyphae but not on infected hosts with visible hyphae. Our findings suggest that I. fumosorosea could detrimentally affect T. radiata, if both natural enemies are simultaneously deployed for biological control of D. citri. However, temporal separation of the fungus and parasitoid could reduce antagonism and enhance control of D. citri. C1 [Chow, Andrew; Setamou, Mamoudou] Texas A&M Univ Kingsville, Citrus Ctr, Weslaco, TX 78596 USA. [Dunlap, Christopher A.; Jackson, Mark A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Flores, Daniel] USDA APHIS PPQ S&T CPHST Mission Lab, Edinburg, TX 78451 USA. [Patt, Joseph M.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Chow, A (reprint author), Texas A&M Univ Kingsville, Citrus Ctr, Weslaco, TX 78596 USA. EM andrew.chow@tamuk.edu; christopher.dunlap@ars.usda.gov; mark.jackson@ars.usda.gov; daniel.flores@aphis.usda.gov; joseph.patt@ars.usda.gov; kums2006@tamuk.edu FU Citrus Research Board [5500-188]; Texas Citrus Producers Board [14405, 14365] FX This research was funded in part by the Citrus Research Board grant no. 5500-188 and the Texas Citrus Producers Board agreement nos. 14405 and 14365. NR 76 TC 1 Z9 1 U1 12 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 1995 EP 2005 DI 10.1093/jee/tow164 PG 11 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700005 PM 27435930 ER PT J AU Bai, GY Xu, H Fu, YQ Wang, XY Shen, GS Ma, HK Feng, X Pan, J Gu, XS Guo, YZ Ruan, WB Shapiro-Ilan, DI AF Bai, Guang-Ying Xu, Hua Fu, Ya-Qi Wang, Xiao-Ying Shen, Guang-Shuang Ma, Hai-Kun Feng, Xiaoling Pan, Jie Gu, Xi-Shu Guo, Yong-Ze Ruan, Wei-Bin Shapiro-Ilan, David I. TI A Comparison of Novel Entomopathogenic Nematode Application Methods for Control of the Chive Gnat, Bradysia odoriphaga (Diptera: Sciaridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE entomopathogenic nematode; nematode suspension; cadaver; biological control; Heterorhabditis bacteriophora ID INFECTED-HOST CADAVERS; ALFALFA SNOUT BEETLE; IN-VIVO PRODUCTION; HETERORHABDITIS-BACTERIOPHORA; STEINERNEMA-CARPOCAPSAE; BIOLOGICAL-CONTROL; AQUEOUS SUSPENSION; RHABDITIDA STEINERNEMATIDAE; DIAPREPES-ABBREVIATUS; WHITE GRUBS AB Bradysia odoriphaga Yang & Zhang (Diptera: Sciaridae) is the most serious pest of Chinese chive, Allium tuberosum. Chemical pesticide application has become a necessary step to control B. odoriphaga after each of the four to six harvests during the growing season. We compared the effects of application type (nematode-infected cadaver, aqueous nematode suspension, and mixture of cadaver and aqueous suspension) and nematode species (Heterorhabditis bacteriophora vs. Steinernema carpocapsae) on B. odoriphaga control. Nematode species combinations and the use of the cadaver method has only been tested in a relatively few studies, and has not been tested for this target pest, B. odoriphaga. Furthermore, this is the first report of combining application methods (aqueous and cadaver). Results indicated that the cadaver treatment produced higher mortality of B. odoriphaga than the aqueous treatment, and H. bacteriophora caused higher mortality of B. odoriphaga than S. carpocapsae. The mortality of B. odoriphaga was 96.7% in H. bacteriophora cadaver treatment and only 27.5% in control treatment without nematode addition. Cadaver treatments caused higher biomass of A. tuberosum than that of aqueous treatment. The plant biomass in H. bacteriophora cadaver treatment was 10 folds as much as that in the control treatment. The mixed aqueous suspension of H. bacteriophora and S. carpocapsae did not increase the mortality in pot trial. Our findings indicate that entomopathogenic nematodes applied as cadavers might be a potential alternative biological agent in the integrated management of B. odoriphaga for Chinese chive production. C1 [Bai, Guang-Ying; Xu, Hua; Fu, Ya-Qi; Wang, Xiao-Ying; Shen, Guang-Shuang; Ma, Hai-Kun; Feng, Xiaoling; Ruan, Wei-Bin] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. [Pan, Jie] Tianjin Inst Agr Resources & Environm Sci, Tianjin 300192, Peoples R China. [Gu, Xi-Shu] Tianjin Inst Plant Protect, Tianjin 300192, Peoples R China. [Guo, Yong-Ze] Inst Agr Qual Stand & Testing Technol, Tianjin 300381, Peoples R China. [Shapiro-Ilan, David I.] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Ruan, WB (reprint author), Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China. EM 337910061@qq.com; 1273782392@qq.com; 542138298@qq.com; 1013983070@qq.com; 76005625@qq.com; 809305111@qq.com; 1053091030@qq.com; 1097778605@qq.com; 13752345430@163.com; guoyz1971@126.com; ruanweibin2004@hotmail.com; David.Shapiro@ars.usda.gov FU Tianjin Natural Science foundation [10JCZDJC7700]; special fund project of Transformation of Scientific and Technological Achievements in Tianjin [201001230]; 111 project [B08011]; funds for national basic science personnel training [J1103503]; Special Fund for Agro-Scientific Research of the Public Interest of PR China [201303088-07] FX This research was jointly supported by the Tianjin Natural Science foundation (10JCZDJC7700) and the special fund project of Transformation of Scientific and Technological Achievements in Tianjin (201001230), and 111 project (B08011), and the funds for national basic science personnel training (J1103503) and Special Fund for Agro-Scientific Research of the Public Interest of PR China (201303088-07). NR 52 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 2006 EP 2013 DI 10.1093/jee/tow162 PG 8 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700006 PM 27480971 ER PT J AU Kumar, S Yee, WL Neven, LG AF Kumar, Sunil Yee, Wee L. Neven, Lisa G. TI Mapping Global Potential Risk of Establishment of Rhagoletis pomonella (Diptera: Tephritidae) Using MaxEnt and CLIMEX Niche Models SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Apple maggot; biosecurity; niche modeling; quarantine pest; pest risk analysis ID APPLE MAGGOT DIPTERA; MOTH LEPIDOPTERA-TORTRICIDAE; SPECIES DISTRIBUTION MODELS; CHERRY FRUIT-FLY; ECOLOGICAL NICHE; GEOGRAPHIC-DISTRIBUTION; SHORT PHOTOPERIOD; CLIMATE SURFACES; ADULT EMERGENCE; PLANT AB The apple maggot, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a major quarantine pest of apples Malus domestica Borkhausen) in the United States. Apple maggot is found only in North America and negatively impacts the apple industry in the western United States by reducing grower access to export markets. To reduce the threat of apple maggot to export countries and to facilitate the movement of commercial apples, an assessment of potential risk of establishment of apple maggot is needed to predict which regions are suitable or unsuitable for the fly. We used a correlative niche model MaxEnt and a mechanistic model CLIMEX to model global potential risk of establishment of apple maggot. The MaxEnt model was developed by integrating apple maggot occurrences with global climatic variables. Apple (a major host of apple maggot) climatic suitability was used as an additional variable to include species interactions in the MaxEnt model. The CLIMEX model was developed using published apple maggot physiological tolerance thresholds. Both the MaxEnt and CLIMEX models correctly predicted the known distribution of apple maggot in North America, met biological expectations when projected to the world, and mostly agreed on climatic suitability worldwide for the fly. Degree-days at 6.7 A degrees C, elevation, precipitation seasonality, and apple climatic suitability were the most important predictors associated with apple maggot distribution in North America. Our results can be used to make science-based international trade decisions by policy makers, and for monitoring apple maggot potential introductions in countries where it currently does not occur. C1 [Kumar, Sunil] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Yee, Wee L.; Neven, Lisa G.] ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Kumar, S (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. EM sunilkrv@gmail.com; Wee.Yee@ars.usda.gov; Lisa.Neven@ars.usda.gov FU Washington Tree Fruit Research Commission (WTFRC) from Foreign Agricultural Service of the United States Department of Agriculture (USDA) FX This research was funded by a grant through the Washington Tree Fruit Research Commission (WTFRC) from the Foreign Agricultural Service of the United States Department of Agriculture (USDA). We thank Mike Willett (Northwest Horticultural Council, WTFRC), Kathy Coffey (WTFRC), Janine Jewett, Pete Chapman, Anne Kenny Chapman, and Shelly Watkins (USDA-Agricultural Research Service) for assistance during the project. We thank Mike Klaus, Brant Carman, and Tom Sandoe of the Washington State Department of Agriculture (WSDA) for providing apple maggot occurrence data. We thank Manuel Colunga (USDA-Animal Plant and Health Inspection Service-Plant Protection and Quarantine-Center for Plant Health Science and Technology-Plant Epidemiology and Risk Analysis Laboratory [ USDA-APHIS-PPQ-CPHST-PERAL]) and Tewodros Wakie (Colorado State University) for their review of the draft manuscript. We also thank the Natural Resource Ecology Laboratory at Colorado State University and Yakima Agricultural Research Laboratory, USDA-ARS, Wapato, Washington, for providing the logistical support. Finally, we are grateful to two anonymous reviewers whose comments improved the manuscript. NR 79 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 2043 EP 2053 DI 10.1093/jee/tow166 PG 11 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700011 PM 27452001 ER PT J AU Nagoshi, RN Gilligan, TM Brambila, J AF Nagoshi, Rodney N. Gilligan, Todd M. Brambila, Julieta TI Combining Tpi and CO1 Genetic Markers to Discriminate Invasive Helicoverpa armigera From Local Helicoverpa zea (Lepidoptera: Noctuidae) Populations in the Southeastern United States SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE cytochrome oxidase 1; triosephosphate isomerase; invasive pest; Helicoverpa armigera; Helicoverpa zea ID FALL ARMYWORM LEPIDOPTERA; PHOSPHATE ISOMERASE GENE; HUBNER LEPIDOPTERA; HELIOTHIS-ZEA; IDENTIFICATION; STRAINS; DIFFERENTIATION; ARGENTINA; BRAZIL AB The recent establishment of the Old World pest Helicoverpa armigera (Hubner) into South America has had significant economic consequences and places the rest of the hemisphere at risk, emphasizing the need for improved methods of monitoring. A major complication is that a sibling species endemic to the New World, Helicoverpa zea (Boddie), is morphologically very similar, with the two species capable of producing fertile hybrids in the laboratory. The consequences of such hybridization in the field are uncertain, but could result in significant and unpredictable changes in the timing, range, and pesticide susceptibilities of Helicoverpa infestations. The objective here is to provide new genetic resources applicable to Helicoverpa populations in northern Florida and neighboring states (a region at risk for H. armigera) that can distinguish the two species and possible hybrids. The genetic variability in segments of the mitochondrial cytochrome oxidase 1 (CO1) and the Z-linked triosephosphate isomerase (Tpi) genes were determined for H. zea from the southeastern United States. These were compared to DNA sequences from H. armigera specimens from Morocco, Australia, and Europe. Phylogenetic network analysis showed a clear demarcation between the two species for all gene segments. These results extend earlier studies establishing CO1 as marker for discriminating the Helicoverpa species complex and introduce a new sex-linked genomic marker. The CO1 and Tpi markers in combination provide a more accurate and sensitive method than existing techniques for identifying hybridization between H. zea and H. armigera and could potentially be used to extrapolate the likely source of invasive H. armigera populations. C1 [Nagoshi, Rodney N.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Gilligan, Todd M.] APHIS, Identificat Technol Program, USDA, PPQ,S&T, 2301 Res Blvd, Ft Collins, CO 80526 USA. [Brambila, Julieta] APHIS, Reg Domest Identifier, Entomol, USDA,PPQ, 1911 SW 34th St, Gainesville, FL 32608 USA. RP Nagoshi, RN (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov; Todd.M.Gilligan@aphis.usda.gov; julieta.brambila@aphis.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209] FX We thank Dr. Steven Valles (USDA-ARS) and Dr. Robert Meagher (USDA-ARS) for important suggestions for the manuscript. We thank Joaquin Baixeras, Michael Pogue, and Marja J. van der Straten for providing specimens of H. armigera used in this study. We recognize the financial support of the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement No. 2011-67003-30209. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 39 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 2115 EP 2124 DI 10.1093/jee/tow177 PG 10 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700020 PM 27551148 ER PT J AU Hansen, EM Munson, AS Blackford, DC Wakarchuk, D Baggett, LS AF Hansen, E. Matthew Munson, A. Steven Blackford, Darren C. Wakarchuk, David Baggett, L. Scott TI Lethal Trap Trees and Semiochemical Repellents as Area Host Protection Strategies for Spruce Beetle (Coleoptera: Curculionidae, Scolytinae) in Utah SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE pheromone; bark beetle management; Dendroctonus rufipennis; carbaryl; MCH ID MOUNTAIN PINE-BEETLE; DENDROCTONUS-PONDEROSAE; ROCKY-MOUNTAINS; VERBENONE; LODGEPOLE; METHYLCYCLOHEXENONE; AGGREGATION; ATTRACTION; REPRESSION; MANAGEMENT AB We tested lethal trap trees and repellent semiochemicals as area treatments to protect host trees from spruce beetle (Dendroctonus rufipennis Kirby) attacks. Lethal trap tree treatments ("spray treatment") combined a spruce beetle bait with carbaryl treatment of the baited spruce. Repellent treatments ("spray-repellent") combined a baited lethal trap tree within a 16-m grid of MCH (3-methylcyclohex-2-en-1-one) and two novel spruce beetle repellents. After beetle flight, we surveyed all trees within 50 m of plot center, stratified by 10-m radius subplots, and compared attack rates to those from baited and unbaited control plots. Compared to the baited controls, spruce in the spray treatment had significantly reduced likelihood of a more severe attack classification (e.g., mass-attacked over strip-attacked or unsuccessful-attacked over unattacked). Because spruce in the spray treatment also had significantly heightened probability of more severe attack classification than those in the unbaited controls, however, we do not recommend lethal trap trees as a stand-alone beetle suppression strategy for epidemic beetle populations. Spruce in the spray-repellent treatment were slightly more likely to be classified as more severely attacked within 30 m of plot center compared to unbaited controls but, overall, had reduced probabilities of beetle attack over the entire 50-m radius plots. The semiochemical repellents deployed in this study were effective at reducing attacks on spruce within treated plots despite the presence of a centrally located spruce beetle bait. Further testing will be required to clarify operational protocols such as dose, elution rate, and release device spacing. C1 [Hansen, E. Matthew] US Forest Serv, Rocky Mt Res Stn, 860 North 1200 East, Logan, UT 84321 USA. [Munson, A. Steven; Blackford, Darren C.] US Forest Serv, Forest Hlth Protect, 4746 South 1900 East, Ogden, UT 84403 USA. [Wakarchuk, David] Synergy Semiochem Corp, Box 50008 South Slope RPO, Burnaby, BC V5J 5G3, Canada. [Baggett, L. Scott] US Forest Serv, Rocky Mt Res Stn, 240 West Prospect St, Ft Collins, CO 80526 USA. RP Hansen, EM (reprint author), US Forest Serv, Rocky Mt Res Stn, 860 North 1200 East, Logan, UT 84321 USA. EM matthansen@fs.fed.us; smunson@fs.fed.us; dblackford@fs.fed.us; david@semiochemical.com; lsbaggett@fs.fed.us FU Forest Service-Pesticide Impact Assessment Program FX Technical help for plot installations and measurements was provided by Amanda Townsend, Jim Vandygriff, Valerie DeBlander, Andrew Giunta, Rob Cruz, and Jason Neumann. Danielle Malesky helped identify suitable study sites. Barbara Bentz and two anonymous reviewers provided helpful comments for previous versions of this manuscript. Thanks to Kathy Johnson and Andrew Orlemann (Fishlake National Forest) and Kristy Groves and Colette Webb (Ashley National Forest) for their cooperation. This study was funded by the Forest Service-Pesticide Impact Assessment Program. Semiochemicals were donated by Synergy Semiochemicals and carbaryl was donated by Chris Olsen, Bayer CropScience. NR 39 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 2137 EP 2144 DI 10.1093/jee/tow172 PG 8 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700022 ER PT J AU Kakkar, G Chouvenc, T Osbrink, W Su, NY AF Kakkar, G. Chouvenc, T. Osbrink, W. Su, N. -Y. TI Temporal Assessment of Molting in Workers of Formosan Subterranean Termites (Isoptera: Rhinotermitidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE stadium; ecdysis; integument ID COPTOTERMES-FORMOSANUS; RETICULITERMES-FLAVIPES; BAIT; NOVIFLUMURON; HEXAFLUMURON; POPULATIONS; SHIRAKI; TEMPERATURES; TERMITICIDES; ELIMINATION AB Molt frequency of workers in laboratory-reared juvenile colonies and foraging population from field colonies of Coptotermes formosanus Shiraki was determined using planar arenas in laboratory. Given that, chitin synthesis inhibitor (CSI)-incorporated baits disrupt the molting process of workers that comprises the major population of a termite colony, temporal assessment of molting frequency in workers can give insights into potential methods of reducing the time to eliminate a CSI-baited colony. In our study the 10-d observation of juvenile colonies of C. formosanus suggested average daily molting incidence of workers in a colony is 1.7 +/- 0.3% (mean +/- SD). The results from a time lapse study on foraging population of workers showed that on average there is a 44-d intermolt period for second-instar workers molting to third instar and 45 d for third-instar workers molting to fourth instar. At low temperature (21 degrees C), molting frequency of workers (0.6% per day) was significantly lower than that of workers at 27 degrees C (2.2% per day). Information from this study suggests that time to molt is an important component of total time for eliminating colonies treated with CSI baits and reduction in time lapse between two consecutive molts may reduce the time required for colony elimination. C1 [Kakkar, G.; Chouvenc, T.; Su, N. -Y.] Univ Florida, Inst Food & Agr Sci, Ft Lauderdale Res & Educ Ctr, Dept Entomol & Nematol, Davie, FL 33314 USA. [Osbrink, W.] USDA ARS, US Livestock Insects Res Lab, SPA Knipling Bushland, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. RP Kakkar, G (reprint author), Univ Florida, Inst Food & Agr Sci, Ft Lauderdale Res & Educ Ctr, Dept Entomol & Nematol, Davie, FL 33314 USA. EM garimaiari@ufl.edu; tomchouv@ufl.edu; weste.osbrink@ars.usda.gov; nysu@ufl.edu FU U.S. Department of Agriculture National Institute of Food and Agriculture, Hatch [FLA-FTL-005342] FX We are grateful to Aaron Mullins and Sarah Bernard (University of Florida) for review of the manuscript and Ronald Pepin (University of Florida) for technical assistance. This work was supported in part by the U.S. Department of Agriculture National Institute of Food and Agriculture, Hatch Project number FLA-FTL-005342. NR 37 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD OCT PY 2016 VL 109 IS 5 BP 2175 EP 2181 DI 10.1093/jee/tow180 PG 7 WC Entomology SC Entomology GA DZ8QU UT WOS:000386136700027 PM 27567218 ER PT J AU Maron, LG Pineros, MA Kochian, LV McCouch, SR AF Maron, Lyza G. Pineros, Miguel A. Kochian, Leon V. McCouch, Susan R. TI Redefining 'stress resistance genes', and why it matters SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Editorial Material DE Aluminum; gene; gene expression; ontology; resistance; stress; stress resistance gene; tolerance ID ALUMINUM-RESISTANCE; ACID SOILS; KNOCKOUT; ADAPTATION; PHENOTYPES; CITRATE; EFFLUX; MICE AB Many plant biologists work on the identification of genes related to abiotic stress resistance, but the term 'stress resistance gene' is widely used without proper definition. Here it is argued that there is a need to update our understanding of this term and for standardization to facilitate integration of research data. C1 [Maron, Lyza G.; McCouch, Susan R.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Pineros, Miguel A.; Kochian, Leon V.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. [Kochian, Leon V.] Univ Saskatchewan, Global Inst Food Secur, Saskatoon, SK S7N 5A8, Canada. RP Maron, LG; McCouch, SR (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. EM lyza.maron@cornell.edu; srm4@cornell.edu RI Maron, Lyza/B-5644-2008; OI Maron, Lyza/0000-0001-6475-2357; Pineros, Miguel/0000-0002-7166-1848 NR 18 TC 1 Z9 1 U1 11 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD OCT PY 2016 VL 67 IS 19 BP 5588 EP 5591 DI 10.1093/jxb/erw323 PG 4 WC Plant Sciences SC Plant Sciences GA EA5JM UT WOS:000386656000002 PM 27702993 ER PT J AU Kale, MS Yadav, MP Hanah, KA AF Kale, Madhuvanti S. Yadav, Madhav P. Hanah, Kyle A. TI Suppression of Psyllium Husk Suspension Viscosity by Addition of Water Soluble Polysaccharides SO JOURNAL OF FOOD SCIENCE LA English DT Article DE dietary fiber; psyllium; viscosity ID LOCUST BEAN GUM; SEED HUSK; GELLING PROPERTIES; HYPERCHOLESTEROLEMIA; IMPROVE; WEIGHT; GEL AB Psyllium seed husk is an insoluble dietary fiber with many health benefits. It can absorb many times its weight in water, forming very viscous suspensions, which have low palatability and consumer acceptance. We report here a novel approach for decreasing its viscosity, involving inclusion of a soluble polysaccharide in the suspension. This leads to a drastic decrease (up to 87%) in viscosity of suspensions, while maintaining the same dosage level of psyllium and also delivering a significant amount of soluble dietary fiber such as corn bio-fiber gum in a single serving. Four soluble polysaccharides with a range of molecular weights and solution viscosities have been studied for their viscosity suppression effect. Besides improving palatability, another advantage of this approach is that it makes it possible to deliver 2 different dietary fibers in significant quantities, thus offering even greater health benefits. Practical Application This study outlines an approach to decrease the viscosity of psyllium husk suspensions in water without chemical, physical or enzymatic modification of the psyllium itself. Addition of a soluble polysaccharide, which may also be a soluble dietary fiber, leads to a drastic decrease in psyllium suspension viscosity, due to reduced swelling of the psyllium particles. Due to this lower viscosity, higher doses of psyllium can be delivered, along with significant amounts of soluble dietary fiber, in a single serving of a psyllium-based dietary fiber supplement, thus offering even greater health benefits than existing products while improving palatability. C1 [Kale, Madhuvanti S.; Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Kale, Madhuvanti S.; Hanah, Kyle A.] Agritech Worldwide Inc, Mundelein, IL 60060 USA. [Kale, Madhuvanti S.] ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94706 USA. RP Kale, MS (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA.; Kale, MS (reprint author), Agritech Worldwide Inc, Mundelein, IL 60060 USA.; Kale, MS (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94706 USA. EM mkale@agrtch.com NR 23 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD OCT PY 2016 VL 81 IS 10 BP E2476 EP E2483 DI 10.1111/1750-3841.13438 PG 8 WC Food Science & Technology SC Food Science & Technology GA DZ3AN UT WOS:000385715500024 PM 27636880 ER PT J AU Milczarek, RR Avena-Mascareno, R Alonzo, J Fichot, MI AF Milczarek, Rebecca R. Avena-Mascareno, Roberto Alonzo, Jerome Fichot, Melissa I. TI Improving the Sun Drying of Apricots (Prunus armeniaca) with Photo-Selective Dryer Cabinet Materials SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antioxidant activity; photo-selective; quality; solar drying; stone fruit ID ANTIOXIDANT CAPACITY; QUALITY CHARACTERISTICS; APPLE SLICES; RADIATION; DEHYDRATION; STORAGE; LEAVES; FRESH; COLOR; HEAT AB Photo-selective materials have been studied for their effects on the preharvest quality of horticultural crops, but little work has been done on potential postharvest processing effects. The aim of this work was to characterize the effects of 5 different photo-selective acrylic materials (used as the lid to a single-layer sun drying cabinet) on the drying rate and quality of apricots (Prunus armeniaca). Photo-selective cabinet materials that transmit light in the visible portion of the solar spectrum accelerate the apricots' drying rate in both the early period of drying and the course of drying as a whole. These materials do not significantly affect the measured quality metrics during the first day of sun drying. However, when drying is taken to completion, some minor but significant quality differences are observed. Infrared-blocking material produces dried apricot with lower red color, compared to clear, opaque black, and ultraviolet-blocking materials. Clear material produced dried apricot with significantly lower antioxidant activity, compared to black and infrared-blocking materials. Using appropriate photo-selective drying cabinet materials can reduce the required sun drying time for apricots by 1 to 2 d, compared with fully shaded drying. Ultraviolet-blocking material is recommended to maximize drying rate and minimize quality degradation. Practical Application This study showed how drying cabinet lid materials that transmitted and blocked different wavelengths from sunlight can speed the drying and improve the quality of dried apricots, increasing the amount of fruit that can be processed in a given length of time without sacrificing nutritional benefits. Apricot drying time could be reduced from 3 to 4 d (shade drying) to 1 to 2 d (drying with clear or ultraviolet-light-blocking materials). The cabinet material did not have an effect on brightness of the fruit or vitamin C content, but it did affect red color and antioxidant activity. C1 [Milczarek, Rebecca R.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Avena-Mascareno, Roberto] Calif State Univ Sacramento, Dept Mech Engn, 6000 J St, Sacramento, CA 95819 USA. [Alonzo, Jerome] AgroSup Dijon, 26 Blvd Petitjean,BP 87999, F-21079 Dijon, France. [Fichot, Melissa I.] INP Purpan, 75 Voie Toec,BP 57611, F-31076 Toulouse 3, France. RP Milczarek, RR (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM rebecca.milczarek@ars.usda.gov NR 27 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD OCT PY 2016 VL 81 IS 10 BP E2466 EP E2475 DI 10.1111/1750-3841.13444 PG 10 WC Food Science & Technology SC Food Science & Technology GA DZ3AN UT WOS:000385715500023 PM 27648527 ER PT J AU Zielin, SB Littlejohn, J de Rivera, CE Smith, WP Jacobson, SL AF Zielin, Sara B. Littlejohn, Jalene de Rivera, Catherine E. Smith, Winston P. Jacobson, Sandra L. TI Ecological investigations to select mitigation options to reduce vehicle-caused mortality of a threatened butterfly SO JOURNAL OF INSECT CONSERVATION LA English DT Article DE Animal-vehicle collisions; Behavior; Habitat connectivity; Mitigation measures; Oregon silverspot butterfly ID ROAD MORTALITY; UNITED-STATES; DIURNAL MOTHS; VERGES; POPULATIONS; MOVEMENT; MANAGEMENT; CONSERVATION; LANDSCAPES; COLLISIONS AB Whereas roads that bisect habitat are known to decrease population size through animal-vehicle collisions or interruption of key life history events, it is not always obvious how to reduce such impacts, especially for flying organisms. We needed a quick, cost-efficient and effective way to determine how best to decrease vehicle-caused mortality while maintaining habitat connectivity for the federally listed Oregon silverspot butterfly, Speyeria zerene hippolyta. Therefore, we gathered targeted ecological information that informed selection of a mitigation option prior to implementation. We sampled butterfly behavior and environmental conditions along a highway and conducted a small-scale experiment along a decommissioned road corridor used by these butterflies. Using our findings, we recommended vegetation management and helped managers eliminate options they were considering that would be ineffective such as increasing shade or wind in the road, and installing fencing or hedgerows aimed at directing flight above traffic. This quick and inexpensive approach of using ecological observations and small-scale experiments to evaluate the likely success of each available mitigation option can be used to determine effective, species-specific solutions for reducing traffic impacts on pollinators and other small, flying organisms of conservation concern. C1 [Zielin, Sara B.; Littlejohn, Jalene; de Rivera, Catherine E.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. [Smith, Winston P.] US Forest Serv, USDA, Pacific Northwest Res Stn, La Grande, OR 97850 USA. [Jacobson, Sandra L.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP de Rivera, CE (reprint author), Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. EM derivera@pdx.edu FU Portland State University FX We thank Martin Bray, Paul Hammond, Randy Miller, Mike Patterson, Melissa Shelley, Paul G. Thomas, and Anne Walker for their generous support and expertise and Chris Parker and Patrick Edwards for help with statistical analyses. Portland State University funded the project (FEA grant to CED) and the USDA Forest Service provided housing as well as field support. NR 61 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1366-638X EI 1572-9753 J9 J INSECT CONSERV JI J. Insect Conserv. PD OCT PY 2016 VL 20 IS 5 BP 845 EP 854 DI 10.1007/s10841-016-9916-4 PG 10 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA EA6DC UT WOS:000386714700010 ER PT J AU Wilson, JS Jahner, JP Starley, L Calvin, CL Ikerd, H Griswold, T AF Wilson, Joseph S. Jahner, Joshua P. Starley, Lisa Calvin, Carmelle L. Ikerd, Harold Griswold, Terry TI Sampling bee communities using pan traps: alternative methods increase sample size SO JOURNAL OF INSECT CONSERVATION LA English DT Article DE Bee declines; Trapping methods; Pan traps; Bowl traps; Nectar guides ID HYMENOPTERA; APIFORMES; DIVERSITY; APOIDEA; MODELS; DESERT; PLANT; FAUNA AB Bees play an important role in natural and agricultural landscapes and increased interest in these pollinators has lead to an increase in studies designed to monitor bee populations across the globe. Many studies investigating bee diversity use pan traps (colored plastic bowls filled with soapy water) as a cost-effective trapping method. Here we investigate how alternative pan trap designs (both the size of the traps, and the addition of "nectar guides") affect the number of specimens collected. We find that larger pan traps collect more specimens than small and medium sized traps, and that the addition of "nectar guides" can significantly increase the number of specimens collected. Increased sample sizes can lead to a better understanding of patterns of bee diversity, which can lead to more informed management decisions. C1 [Wilson, Joseph S.; Starley, Lisa; Calvin, Carmelle L.] Utah State Univ Tooele, Dept Biol, 1021 West Vine St, Tooele, UT 84074 USA. [Jahner, Joshua P.] Univ Nevada, Program Ecol Evolut & Conservat Biol, Dept Biol, Reno, NV 89557 USA. [Ikerd, Harold; Griswold, Terry] USDA ARS, Bee Biol & Systemat Lab, 5310 Old Main Hill, Logan, UT 84322 USA. RP Wilson, JS (reprint author), Utah State Univ Tooele, Dept Biol, 1021 West Vine St, Tooele, UT 84074 USA. EM joeswilson@gmail.com NR 23 TC 0 Z9 0 U1 8 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1366-638X EI 1572-9753 J9 J INSECT CONSERV JI J. Insect Conserv. PD OCT PY 2016 VL 20 IS 5 BP 919 EP 922 DI 10.1007/s10841-016-9914-6 PG 4 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA EA6DC UT WOS:000386714700017 ER PT J AU Smelser, A Gardner, C Blanco, M Lubberstedt, T Frei, U AF Smelser, Andrew Gardner, Candice Blanco, Michael Lubberstedt, Thomas Frei, Ursula TI Germplasm enhancement of maize: a look into haploid induction and chromosomal doubling of haploids from temperate-adapted tropical sources SO PLANT BREEDING LA English DT Article DE doubled haploid; GEM; diversity; maize genetic resources ID PLANTS; LINE AB The allelic diversity (AD) project of the Germplasm Enhancement of Maize (GEM) programme utilized the double haploid (DH) breeding method to expedite development and release of lines derived from 300 exotic maize races. Using 18 races in this study, differential effects on haploid induction rates (HIRs) and doubling rates (DRs) by the recurrent parents PHB47 and PHZ51, the elevation that the race is traditionally grown at, and by the race itself were addressed in this study. Races from the AD project were grouped by elevation of their origin, high, middle or low altitude. Six races per elevation were randomly selected and backcrossed using both recurrent parents to generate 36 populations. Ten replications were randomized in a complete randomized design for two growing seasons. The recurrent parent effect was significant, with PHB47 having a higher HIR than PHZ51. Effect of elevation was significant with higher HIR associated with low-elevation origin, and race also proved to be significant. Effects of elevation, recurrent parent and race were not significant for DR. C1 [Smelser, Andrew; Gardner, Candice; Blanco, Michael] USDA ARS, North Cent Reg Plant Intro Stn, 1305 State Ave, Ames, IA 50014 USA. [Smelser, Andrew; Gardner, Candice; Blanco, Michael] Iowa State Univ, Dept Agron, G212 Agron Hall, Ames, IA 50011 USA. [Lubberstedt, Thomas; Frei, Ursula] Iowa State Univ, Dept Agron, 2101 Agron Hall, Ames, IA 50011 USA. RP Gardner, C (reprint author), USDA ARS, North Cent Reg Plant Intro Stn, 1305 State Ave, Ames, IA 50014 USA.; Gardner, C (reprint author), Iowa State Univ, Dept Agron, G212 Agron Hall, Ames, IA 50011 USA. EM candice.gardner@ars.usda.gov FU USDA-ARS; Hatch Multistate Project NC-7; Maize Curator Mark Millard of the North Central Regional Plant Introduction Station FX The authors thank the Iowa State Doubled Haploid Facility, which offers technology services to public and private sector researchers (http://www.plantbreeding.iastate.edu/DHF/DHF.htm); and the Agriculture Experiment Station Consulting Group from the Department of Statistics at Iowa State University for statistical support. We obtain funding support from USDA-ARS and Hatch Multistate Project NC-7 and thank Maize Curator Mark Millard of the North Central Regional Plant Introduction Station, who provided maize racial germplasm from the NPGS maize collection. Many thanks go out to Fred Engstrom, Nuo Shen and Adam Vanous for their technical support in this project. NR 27 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD OCT PY 2016 VL 135 IS 5 BP 593 EP 597 DI 10.1111/pbr.12397 PG 5 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DZ2JY UT WOS:000385669300010 ER PT J AU Liu, YF Li, SJ Chen, Y Kimberlin, AN Cahoon, EB Yu, B AF Liu, Yunfeng Li, Shengjun Chen, Yuan Kimberlin, Athen N. Cahoon, Edgar B. Yu, Bin TI snRNA 3 ' End Processing by a CPSF73-Containing Complex Essential for Development in Arabidopsis SO PLOS BIOLOGY LA English DT Article ID RNA-POLYMERASE-II; SMALL NUCLEAR; INTEGRATOR COMPLEX; U2 SNRNA; 3'-END FORMATION; BINDING-PROTEIN; U6 SNRNA; GENE-EXPRESSION; TERMINAL DOMAIN; TRANSCRIPTION AB Uridine-rich small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play essential roles in splicing. The biogenesis of the majority of snRNAs involves 3' end endonucleolytic cleavage of the nascent transcript from the elongating DNA-dependent RNA ploymerase II. However, the protein factors responsible for this process remain elusive in plants. Here, we show that DEFECTIVE in snRNA PROCESSING 1 (DSP1) is an essential protein for snRNA 30 end maturation in Arabidopsis. A hypomorphic dsp1-1 mutation causes pleiotropic developmental defects, impairs the 30 end processing of snRNAs, increases the levels of snRNA primary transcripts (pre-snRNAs), and alters the occupancy of Pol II at snRNA loci. In addition, DSP1 binds snRNA loci and interacts with Pol-II in a DNA/RNA-dependent manner. We further show that DSP1 forms a conserved complex, which contains at least four additional proteins, to catalyze snRNA 3' end maturation in Arabidopsis. The catalytic component of this complex is likely the cleavage and polyadenylation specificity factor 73 kDa-I (CSPF73-I), which is the nuclease cleaving the pre-mRNA 3' end. However, the DSP1 complex does not affect pre-mRNA 3' end cleavage, suggesting that plants may use different CPSF73-I-containing complexes to process snRNAs and pre-mRNAs. This study identifies a complex responsible for the snRNA 3' end maturation in plants and uncovers a previously unknown function of CPSF73 in snRNA maturation. C1 [Liu, Yunfeng; Li, Shengjun; Yu, Bin] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA. [Liu, Yunfeng; Li, Shengjun; Yu, Bin] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [Chen, Yuan] Univ Calif Berkeley, USDA, Ctr Plant Gene Express, ARS, Albany, CA 94710 USA. [Kimberlin, Athen N.; Cahoon, Edgar B.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE USA. [Kimberlin, Athen N.; Cahoon, Edgar B.] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. RP Yu, B (reprint author), Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA.; Yu, B (reprint author), Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. EM byu3@unl.edu FU National Science Foundation [MCB-1121193] FX This work is supported by a National Science Foundation Grant MCB-1121193 (to BY). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 0 Z9 0 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1545-7885 J9 PLOS BIOL JI PLoS. Biol. PD OCT PY 2016 VL 14 IS 10 AR e1002571 DI 10.1371/journal.pbio.1002571 PG 22 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA EA7NG UT WOS:000386817100006 PM 27780203 ER PT J AU Huang, YJS Hettenbach, SM Park, SL Higgs, S Barrett, ADT Hsu, WW Harbin, JN Cohnstaedt, LW Vanlandingham, DL AF Huang, Yan-Jang S. Hettenbach, Susan M. Park, So Lee Higgs, Stephen Barrett, Alan D. T. Hsu, Wei-Wen Harbin, Julie N. Cohnstaedt, Lee W. Vanlandingham, Dana L. TI Differential Infectivities among Different Japanese Encephalitis Virus Genotypes in Culex quinquefasciatus Mosquitoes SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Article ID WEST-NILE-VIRUS; PIPIENS-QUINQUEFASCIATUS; VECTOR COMPETENCE; DIPTERA-CULICIDAE; LABORATORY TRANSMISSION; MOLECULAR EPIDEMIOLOGY; INFECTION; DISSEMINATION; DIVERSITY; STRAINS AB During the last 20 years, the epidemiology of Japanese encephalitis virus (JEV) has changed significantly in its endemic regions due to the gradual displacement of the previously dominant genotype III (GIII) with clade b of GI (GI-b). Whilst there is only limited genetic difference distinguishing the two GI clades (GI-a and GI-b), GI-b has shown a significantly wider and more rapid dispersal pattern in several regions in Asia than the GI-a clade, which remains restricted in its geographic distribution since its emergence. Although previously published molecular epidemiological evidence has shown distinct phylodynamic patterns, characterization of the two GI clades has only been limited to in vitro studies. In this study, Culex quinquefasciatus, a known competent JEV mosquito vector species, was orally challenged with three JEV strains each representing GI-a, GI-b, and GIII, respectively. Infection and dissemination were determined based on the detection of infectious viruses in homogenized mosquitoes. Detection of JEV RNA in mosquito saliva at 14 days post infection indicated that Cx. quinquefasciatus can be a competent vector species for both GI and GIII strains. Significantly higher infection rates in mosquitoes exposed to the GI-b and GIII strains than the GI-a strain suggest infectivity in arthropod vectors may lead to the selective advantage of previously and currently dominant genotypes. It could thus play a role in enzootic transmission cycles for the maintenance of JEV if this virus were ever to be introduced into North America. C1 [Huang, Yan-Jang S.; Park, So Lee; Higgs, Stephen; Harbin, Julie N.; Vanlandingham, Dana L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Huang, Yan-Jang S.; Hettenbach, Susan M.; Park, So Lee; Higgs, Stephen; Harbin, Julie N.; Vanlandingham, Dana L.] Kansas State Univ, Biosecur Res Inst, Manhattan, KS 66506 USA. [Barrett, Alan D. T.] Univ Texas Med Branch, Dept Pathol, Galveston, TX 77555 USA. [Barrett, Alan D. T.] Univ Texas Med Branch, Sealy Ctr Vaccine Dev, Galveston, TX 77555 USA. [Hsu, Wei-Wen] Kansas State Univ, Coll Arts & Sci, Dept Stat, Manhattan, KS 66506 USA. [Cohnstaedt, Lee W.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS USA. RP Vanlandingham, DL (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA.; Vanlandingham, DL (reprint author), Kansas State Univ, Biosecur Res Inst, Manhattan, KS 66506 USA. EM dlvanlan@vet.ksu.edu FU National Bio and Agro-Defense Facility fund FX The research was supported by the National Bio and Agro-Defense Facility fund to DLV. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 0 Z9 0 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD OCT PY 2016 VL 10 IS 10 AR e0005038 DI 10.1371/journal.pntd.0005038 PG 11 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA EA5QH UT WOS:000386676200027 PM 27706157 ER PT J AU Sikulu-Lord, MT Milali, MP Henry, M Wirtz, RA Hugo, LE Dowell, FE Devine, GJ AF Sikulu-Lord, Maggy T. Milali, Masabho P. Henry, Michael Wirtz, Robert A. Hugo, Leon E. Dowell, Floyd E. Devine, Gregor J. TI Near-Infrared Spectroscopy, a Rapid Method for Predicting the Age of Male and Female Wild-Type and Wolbachia Infected Aedes aegypti SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Article ID MOSQUITO AGE; TRANSCRIPTIONAL PROFILES; CUTICULAR HYDROCARBONS; ZIKA VIRUS; VECTOR; DENGUE; CHIKUNGUNYA; CULICIDAE; DIPTERA AB Estimating the age distribution of mosquito populations is crucial for assessing their capacity to transmit disease and for evaluating the efficacy of available vector control programs. This study reports on the capacity of the near-infrared spectroscopy (NIRS) technique to rapidly predict the ages of the principal dengue and Zika vector, Aedes aegypti. The age of wild-type males and females, and males and females infected with wMel and wMelPop strains of Wolbachia pipientis were characterized using this method. Calibrations were developed using spectra collected from their heads and thoraces using partial least squares (PLS) regression. A highly significant correlation was found between the true and predicted ages of mosquitoes. The coefficients of determination for wild-type females and males across all age groups were R-2 = 0.84 and 0.78, respectively. The coefficients of determination for the age of wMel and wMelPop infected females were 0.71 and 0.80, respectively (P<0.001 in both instances). The age of wild-type female Ae. aegypti could be identified as < or >= 8 days old with an accuracy of 91% (N = 501), whereas female Ae. aegypti infected with wMel and wMelPop were differentiated into the two age groups with an accuracy of 83% (N = 284) and 78% (N = 229), respectively. Our results also indicate NIRS can distinguish between young and old male wild-type, wMel and wMelPop infected Ae. aegypti with accuracies of 87% (N = 253), 83% (N = 277) and 78% (N = 234), respectively. We have demonstrated the potential of NIRS as a predictor of the age of female and male wild-type and Wolbachia infected Ae. aegypti mosquitoes under laboratory conditions. After field validation, the tool has the potential to offer a cheap and rapid alternative for surveillance of dengue and Zika vector control programs. C1 [Sikulu-Lord, Maggy T.; Hugo, Leon E.; Devine, Gregor J.] QIMR Berghofer Med Res Inst, Mosquito Control Lab, Brisbane, Qld, Australia. [Sikulu-Lord, Maggy T.; Milali, Masabho P.; Henry, Michael] Ifakara Hlth Inst, Environm Hlth & Ecol Sci Themat Grp, Ifakara, Tanzania. [Wirtz, Robert A.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Dowell, Floyd E.] ARS, Stored Prod Insect & Engn Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. RP Sikulu-Lord, MT (reprint author), QIMR Berghofer Med Res Inst, Mosquito Control Lab, Brisbane, Qld, Australia.; Sikulu-Lord, MT (reprint author), Ifakara Hlth Inst, Environm Hlth & Ecol Sci Themat Grp, Ifakara, Tanzania. EM maggy.sikulu@qimrberghofer.edu.au FU Grand Challenges Canada Stars for Global Health - Government of Canada [0439-01] FX This study was supported by Grand Challenges Canada Stars for Global Health funded by the Government of Canada (grant 0439-01) awarded to MTSL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 0 Z9 0 U1 11 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD OCT PY 2016 VL 10 IS 10 AR e0005040 DI 10.1371/journal.pntd.0005040 PG 11 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA EA5QH UT WOS:000386676200028 PM 27768689 ER PT J AU Ochyra, R Garland, M AF Ochyra, Ryszard Garland, Mark TI Nomenclatural history of the generic name Haplocladium (Bryophyta: Leskeaceae) SO TAXON LA English DT Article DE bryophytes; Karl (Carl) Muller of Halle; conserved name; intended basionym; Musci; nomenclature; Thuidiaceae; valid publication AB The nomenclatural history of the moss generic name Haplocladium is discussed. Originally, this name was validly published in 1879 by Karl Muller of Halle as a subsection, Hypnum subsect. Haplocladium. It gained generic status in 1897 when the same author provided a brief diagnosis of the new genus Haplocladium. Under Art. 41.4 Haplocladium is treated as a name at a new rank published without reference to the presumably intended basionym Hypnum subsect. Haplocladium. It is shown that the authorship of Haplocladium microphyllum, which was previously attributed to "(Hedw.)Broth.", should correctly be ascribed to "(Hedw.) Mull.Hal." (in Hedwigia 36: 140. Jun 1897). The confusion associated with H. gracile is clarified and the authorship of this name is correctly attributed to "(Schimp.) Mull. Hal." (in Hedwigia 36: 140. Jun 1897). C1 [Ochyra, Ryszard] Polish Acad Sci, Inst Bot, Lab Bryol, Ul Lubicz 46, PL-31512 Krakow, Poland. [Garland, Mark] USDA, Natl Plant Data Team, Nat Resources Conservat Serv, Greensboro, NC 27401 USA. RP Ochyra, R (reprint author), Polish Acad Sci, Inst Bot, Lab Bryol, Ul Lubicz 46, PL-31512 Krakow, Poland. EM r.ochyra@botany.pl FU W. Szafer Institute of Botany of the Polish Academy of Sciences FX The authors are grateful to John McNeill (Royal Botanic Garden, Edinburgh, U.K.), Werner Greuter and Nicholas J. Turland (Botanischer Garten und "Botanisches Museum Berlin-Dahlem, Berlin, Germany) for their valuable discussion and helpful advice on critical nomenclatural issues on the matter. The work of the first author was financed through the statutory fund of the W. Szafer Institute of Botany of the Polish Academy of Sciences. NR 36 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD OCT PY 2016 VL 65 IS 5 BP 1097 EP 1100 DI 10.12705/655.11 PG 4 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA EA4QE UT WOS:000386597600011 ER PT J AU Ochyra, R Garland, M AF Ochyra, Ryszard Garland, Mark TI (2468) Proposal to conserve the name Haplocladium (Mull. Hal.) Mull. Hal. (Bryophyta : Leskeaceae) against Haplocladium Nageli (Rhodophyceae : Ceramiaceae) SO TAXON LA English DT Editorial Material C1 [Ochyra, Ryszard] Polish Acad Sci, Inst Bot, Lab Bryol, Ul Lubicz 46, PL-31512 Krakow, Poland. [Garland, Mark] USDA, Natl Plant Data Team, Nat Resources Conservat Serv, 2901 East Gate City Blvd,Suite 2100, Greensboro, NC 27401 USA. RP Ochyra, R (reprint author), Polish Acad Sci, Inst Bot, Lab Bryol, Ul Lubicz 46, PL-31512 Krakow, Poland. EM r.ochyra@botany.pl NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD OCT PY 2016 VL 65 IS 5 BP 1172 EP + DI 10.12705/655.21 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA EA4QE UT WOS:000386597600021 ER PT J AU Wiersema, JH AF Wiersema, John H. TI (364) Proposal to provide a more direct definition of the term "gathering" SO TAXON LA English DT Editorial Material C1 [Wiersema, John H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. RP Wiersema, JH (reprint author), ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. EM john.wiersema@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD OCT PY 2016 VL 65 IS 5 BP 1186 EP 1186 DI 10.12705/655.32 PG 1 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA EA4QE UT WOS:000386597600032 ER PT J AU Wiersema, JH Gandhi, KN AF Wiersema, John H. Gandhi, Kanchi N. TI (385-388) Proposals to amend Articles 32.2, 23.5, and 24.2 to clarify the treatment of transcribed Greek terminations of epithets SO TAXON LA English DT Editorial Material C1 [Wiersema, John H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. [Gandhi, Kanchi N.] Harvard Univ, Harvard Univ Herbaria, 22 Divin Ave, Cambridge, MA 02138 USA. RP Wiersema, JH (reprint author), ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. EM john.wiersema@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD OCT PY 2016 VL 65 IS 5 BP 1195 EP + DI 10.12705/655.37 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA EA4QE UT WOS:000386597600037 ER PT J AU McNeill, J Shaw, JMH Wiersema, JH AF McNeill, John Shaw, Julian M. H. Wiersema, John H. TI (390) Proposal to preclude homonymy of generic names with names of intergeneric graft-hybrids (chimaeras) SO TAXON LA English DT Editorial Material C1 [McNeill, John] Royal Bot Garden, 20A Inverleith Row, Edinburgh EH3 5LR, Midlothian, Scotland. [Shaw, Julian M. H.] Royal Hort Soc, Hort Taxon, Woking GU23 6QB, Surrey, England. [Wiersema, John H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. RP McNeill, J (reprint author), Royal Bot Garden, 20A Inverleith Row, Edinburgh EH3 5LR, Midlothian, Scotland. EM J.McNeill@rbge.ac.uk NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD OCT PY 2016 VL 65 IS 5 BP 1198 EP 1199 DI 10.12705/655.39 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA EA4QE UT WOS:000386597600039 ER PT J AU Zhou, YC Giusti, MM Parker, J Salamanca, J Rodriguez-Saona, C AF Zhou, Yucheng Giusti, M. Monica Parker, Joyce Salamanca, Jordano Rodriguez-Saona, Cesar TI Frugivory by Brown Marmorated Stink Bug (Hemiptera: Pentatomidae) Alters Blueberry Fruit Chemistry and Preference by Conspecifics SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Brown marmorated stink bug; Vaccinium corymbosum; anthocyanin; phenolic; induced resistance ID PLANT DEFENSE; HERBIVORES; PHENOLICS; RESPONSES; QUALITY; PEST; PHYSIOLOGY; APPLE; ACID AB The brown marmorated stink bug, Halyomorpha halys (StAyenl), is an invasive pest from Asia that feeds on many agricultural crops in the United States, including blueberries. Yet, the effects of H. halys feeding on fruit chemistry and induced resistance to insects remain unknown. Here we hypothesized that frugivory by H. halys changes fruit chemical composition, which in turn affects insect feeding behavior. In field experiments, blueberry fruit was either mechanically injured or injured by 0 (control), 2, 5, or 10 H. halys. Total soluble solids (A degrees Brix) and anthocyanin and phenolic content in injured and uninjured fruits, as well as their effects on feeding behavior by conspecifics, were measured subsequently in the laboratory. Results showed lower A degrees Brix values in injured fruit as compared with uninjured fruit. Fruit injured by 2 and 5 H. halys also had 32 and 20% higher total phenolics, respectively, than the uninjured controls. The proportions of the anthocyanins derived from delphinidin, cyanidin, and petunidin increased, whereas those from malvidin decreased, in fruit after mechanical wounding and frugivory by H. halys. In dual-choice tests, H. halys fed more often on uninjured fruit than those previously injured by conspecifics. These results show that frugivory by H. halys reduces the amounts of soluble solids, alters anthocyanin ratios, and increases levels of phenolics, and, as a result, injured fruits were a less preferred food source for conspecifics. To our knowledge, this is the first study to investigate the effects of frugivory on fruit chemistry and induced fruit resistance against a fruit-eating herbivore. C1 [Zhou, Yucheng; Giusti, M. Monica] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA. [Parker, Joyce] USDA, Natl Inst Food & Agr, Washington, DC 20250 USA. [Salamanca, Jordano] Univ Fed Lavras, Dept Entomologia, BR-37200 Lavras, MG, Brazil. [Rodriguez-Saona, Cesar] Rutgers State Univ, Dept Entomol, New Brunswick, NJ 08901 USA. RP Rodriguez-Saona, C (reprint author), Rutgers State Univ, Dept Entomol, New Brunswick, NJ 08901 USA. EM zhou.1140@osu.edu; giusti.6@osu.edu; Joyce.Parker@nifa.usda.gov; jordanosalamanca@gmail.com; crodriguez@aesop.rutgers.edu FU USDA NIFA SCRI [2011-51181-30937]; New Jersey Agricultural Experiment Station Multistate funds [NJ08270, NJ08225]; hatch project [NJ08192] FX We thank Patty Giron, Amy Blood, and Chris Reale for laboratory and field assistance. Thanks also to Drs. Johnattan Hernandez-Cumplido and Brett Blaauw, and two anonymous reviewers, for comments on an earlier draft of this manuscript. Funds for this project were provided by a USDA NIFA SCRI grant #2011-51181-30937, the New Jersey Agricultural Experiment Station Multistate funds #NJ08270 and #NJ08225, and a hatch project #NJ08192 to C.R-S. NR 49 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2016 VL 45 IS 5 BP 1227 EP 1234 DI 10.1093/ee/nvw110 PG 8 WC Entomology SC Entomology GA DZ7NP UT WOS:000386053100017 PM 27550164 ER PT J AU Reay-Jones, FPF Greene, JK Bauer, PJ AF Reay-Jones, Francis P. F. Greene, Jeremy K. Bauer, Philip J. TI Stability of Spatial Distributions of Stink Bugs, Boll Injury, and NDVI in Cotton SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE spatial analysis by distance indices; spatial aggregation; Chinavia hilaris; Nezara viridula; Euschistus servus ID INTEGRATED PEST-MANAGEMENT; HEMIPTERA-PENTATOMIDAE; COUNT DATA; NATURAL ENEMIES; SAMPLING PLANS; COASTAL-PLAIN; DYNAMICS; PATTERNS; YIELD; CHRYSOMELIDAE AB A 3-yr study was conducted to determine the degree of aggregation of stink bugs and boll injury in cotton, Gossypium hirsutum L., and their spatial association with a multispectral vegetation index (normalized difference vegetation index [NDVI]). Using the spatial analysis by distance indices analyses, stink bugs were less frequently aggregated (17% for adults and 4% for nymphs) than boll injury (36%). NDVI values were also significantly aggregated within fields in 19 of 48 analyses (40%), with the majority of significant indices occurring in July and August. Paired NDVI datasets from different sampling dates were frequently associated (86.5% for weekly intervals among datasets). Spatial distributions of both stink bugs and boll injury were less stable than for NDVI, with positive associations varying from 12.5 to 25% for adult stink bugs for weekly intervals, depending on species. Spatial distributions of boll injury from stink bug feeding were more stable than stink bugs, with 46% positive associations among paired datasets with weekly intervals. NDVI values were positively associated with boll injury from stink bug feeding in 11 out of 22 analyses, with no significant negative associations. This indicates that NDVI has potential as a component of site-specific management. Future work should continue to examine the value of remote sensing for insect management in cotton, with an aim to develop tools such as risk assessment maps that will help growers to reduce insecticide inputs. C1 [Reay-Jones, Francis P. F.] Clemson Univ, Dept Agr & Environm Sci, Pee Dee Res & Educ Ctr, 2200 Pocket Rd, Florence, SC 29506 USA. [Greene, Jeremy K.] Clemson Univ, Dept Agr & Environm Sci, Edisto Res & Educ Ctr, 64 Res Rd, Blackville, SC 29817 USA. [Bauer, Philip J.] USDA, ARS Coastal Plain Soil, Water & Plant Conservat Res, Florence, SC 29201 USA. RP Reay-Jones, FPF (reprint author), Clemson Univ, Dept Agr & Environm Sci, Pee Dee Res & Educ Ctr, 2200 Pocket Rd, Florence, SC 29506 USA. EM freayjo@clemson.edu; GREENE4@clemson.edu; Phil.Bauer@ARS.USDA.GOV FU Cotton Incorporated; NIFA/USDA [SC-1700470, SC-1700455, SC-1700441] FX We thank William Griggs, Terry Teal, Dan Robinson, and James Smoak (Clemson University) for technical help and Monsanto for providing cotton seed and herbicide. We would also like to thank Cotton Incorporated for partially funding this project. Technical Contribution No. 6438 of the Clemson University Experiment Station. This material is based upon work supported by NIFA/USDA, under project number SC-1700470, SC-1700455, and SC-1700441. NR 58 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2016 VL 45 IS 5 BP 1243 EP 1254 DI 10.1093/ee/nvw084 PG 12 WC Entomology SC Entomology GA DZ7NP UT WOS:000386053100019 PM 27436843 ER PT J AU DiMeglio, AS Wallingford, AK Weber, DC Kuhar, TP Mullins, D AF DiMeglio, Anthony S. Wallingford, Anna K. Weber, Donald C. Kuhar, Thomas P. Mullins, Donald TI Supercooling Points of Murgantia histrionica (Hemiptera: Pentatomidae) and Field Mortality in the Mid-Atlantic United States Following Lethal Low Temperatures SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE supercooling point; lethal low temperature; field survival; polar vortex ID INSECT COLD-HARDINESS; HARLEQUIN BUG; TOLERANCE; HETEROPTERA; CLIMATE; BIOLOGY; HAHN AB The harlequin bug, Murgantia histrionica (Hahn), is a serious pest of brassicaceous vegetables in southern North America. While this insect is limited in its northern range of North America, presumably by severe cold winter temperatures, specific information on its cold hardiness remains unknown. We determined the supercooling points (SCPs) for Maryland and Virginia adult populations and found no significant difference among these populations. SCPs were similar for adults ( = -10.35 A degrees C; 2.54) and early and late instar 11.00 C; rX 4.92) and between adult males and females. However, SCPs for first instars 21.56 C; rX 1.47) and eggs (X 23.24 C; rX1.00) were significantly lower. We also evaluated field survival of overwintering harlequin bug adults during extreme cold episodes of January 2014 and January 2015, which produced widespread air temperatures lower than -15 A degrees C and subfreezing soil temperatures in the Mid-Atlantic Region. After the 48-h episode in 2014, bug mortality in exposed field sites averaged 88%, compared to < 5% mortality of bugs sheltered in an unheated greenhouse (recorded minimum temperature 4.4 A degrees C). After the 2015 episode, 80% of adults that were established in the field the previous November and then sheltered in an unheated garage during the episode, died, in contrast to 96% mortality in exposed field sites. Our results provide new information on M. histrionica overwintering biology, and thermal limitations to its distribution, which leads to improved predictive capabilities to forecast pest severity. C1 [DiMeglio, Anthony S.; Kuhar, Thomas P.; Mullins, Donald] Virginia Tech, Dept Entomol, 170 Drillfield Dr, Blacksburg, VA 24061 USA. [Wallingford, Anna K.] Cornell Univ, Dept Entomol, 630 W North St, Geneva, NY 14456 USA. [Weber, Donald C.] USDA ARS, Invas Insect Biocontrol & Behav Lab, BARC West 007, Beltsville, MD 20705 USA. RP DiMeglio, AS (reprint author), Virginia Tech, Dept Entomol, 170 Drillfield Dr, Blacksburg, VA 24061 USA. EM harlequinbugs@vt.edu; annawllngfrd@gmail.com; Don.Weber@ars.usda.gov; tkuhar@vt.edu; mullinsd@vt.edu NR 46 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD OCT PY 2016 VL 45 IS 5 BP 1294 EP 1299 DI 10.1093/ee/nvw091 PG 6 WC Entomology SC Entomology GA DZ7NP UT WOS:000386053100025 PM 27497116 ER PT J AU Root, HT Dodson, EK AF Root, Heather T. Dodson, Erich Kyle TI Pssst ... pass the algae: succession in lichen soil crusts SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Editorial Material C1 [Root, Heather T.] Weber State Univ, Dept Bot, Ogden, UT 84408 USA. [Dodson, Erich Kyle] US Forest Serv, Rocky Mt Res Stn, USDA, Ogden, UT USA. RP Root, HT (reprint author), Weber State Univ, Dept Bot, Ogden, UT 84408 USA. EM heatherroot@weber.edu NR 0 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD OCT PY 2016 VL 14 IS 8 BP 451 EP 452 DI 10.1002/fee.1419 PG 2 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DZ8MD UT WOS:000386123600017 ER PT J AU Hinchliffe, DJ Condon, BD Thyssen, G Naoumkina, M Madison, CA Reynolds, M Delhom, CD Fang, DD Li, P McCarty, J AF Hinchliffe, Doug J. Condon, Brian D. Thyssen, Gregory Naoumkina, Marina Madison, Crista A. Reynolds, Michael Delhom, Christopher D. Fang, David D. Li, Ping McCarty, Jack TI The GhTT2_A07 gene is linked to the brown colour and natural flame retardancy phenotypes of Lc1 cotton (Gossypium hirsutum L.) fibres SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Lc1 locus; brown fibre; cotton; fame retardant; flavonoid; proanthocyanidin; textiles; transparent testa ID GERMPLASM LINES; UPLAND COTTON; PIGMENTATION; REGISTRATION; VARIANTS; SEQUENCE; QUALITY; FABRICS; LOCUS AB The brown fibre cotton Lc1 locus is linked to a 1.4Mb genomic inversion that activates GhTT2_A07. This mutation upregulates flavonoid biosynthesis and confers natural flame retardancy.Some naturally coloured brown cotton fibres from accessions of Gossypium hirsutum L. can be used to make textiles with enhanced flame retardancy (FR). Several independent brown fibre loci have been identified and mapped to chromosomes, but the underlying genes have not yet been identified, and the mechanism of lint fibre FR is not yet fully understood. In this study, we show that both the brown colour and enhanced FR of the Lc1 lint colour locus are linked to a 1.4Mb inversion on chromosome A07 that is immediately upstream of a gene with similarity to Arabidopsis TRANSPARENT TESTA 2 (TT2). As a result of the alternative upstream sequence, the transcription factor GhTT2_A07 is highly up-regulated in developing fibres. In turn, genes in the phenylpropanoid metabolic pathway are activated, leading to biosynthesis of proanthocyanidins and accumulation of inorganic elements. We show that enhanced FR and anthocyanin precursors appear in developing brown fibres well before the brown colour is detectible, demonstrating for the first time that the polymerized proanthocyanidins that constitute the brown colour are not the source of enhanced FR. Identifying the particular colourless metabolite that provides Lc1 cotton with enhanced FR could help minimize the use of synthetic chemical flame retardant additives in textiles. C1 [Hinchliffe, Doug J.; Condon, Brian D.; Thyssen, Gregory; Madison, Crista A.; Reynolds, Michael] USDA, Agr Res Serv, Southern Reg Res Ctr, Cotton Chem & Utilizat Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Naoumkina, Marina; Fang, David D.; Li, Ping] USDA, Agr Res Serv, Southern Reg Res Ctr, Cotton Fiber Biosci Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Delhom, Christopher D.] USDA, Agr Res Serv, Southern Reg Res Ctr, Cotton Struct & Qual Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [McCarty, Jack] USDA, Agr Res Serv, Genet & Sustainable Agr Res Unit, Mississippi State, MS 39762 USA. RP Hinchliffe, DJ (reprint author), USDA, Agr Res Serv, Southern Reg Res Ctr, Cotton Chem & Utilizat Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM doug.hinchliffe@ars.usda.gov NR 46 TC 0 Z9 0 U1 8 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD OCT PY 2016 VL 67 IS 18 BP 5461 EP 5471 DI 10.1093/jxb/erw312 PG 11 WC Plant Sciences SC Plant Sciences GA DZ7SO UT WOS:000386067000016 PM 27567364 ER PT J AU Reich, RM Lundquist, JE Hughes, K AF Reich, Robin M. Lundquist, John E. Hughes, Kristina TI Host-environment mismatches associated with subalpine fir decline in Colorado SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Abiotic disorder; Climate stress; High elevation forests; Forest disease; Subalpine fir ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; BALSAM BARK BEETLE; CLIMATE-CHANGE; SPATIAL-DISTRIBUTION; FOREST STRUCTURE; ROOT DISEASE; INSECTS; DROUGHT; JALISCO; MEXICO AB Subalpine fir decline (SFD) has killed more trees in Colorado's high elevation forests than any other insect or disease problem. The widespread nature of this disorder suggests that the cause involves climatic factors. We examined the influence of varying combinations of average annual temperature and precipitation on the incidence and distribution of SFD. Climatic transition matrices generated in this study indicate that most healthy trees are found in climatic zones with moderate to low temperatures and high precipitation; whereas, SFD occurs mostly in zones of moderate temperatures and moderate precipitation. The contrasting distributions define an environmental mismatch. Forests matched with favorable climatic conditions thrive; those that are mismatched can become vulnerable to decline disease. C1 [Reich, Robin M.; Hughes, Kristina] Colorado State Univ, Ft Collins, CO 80523 USA. [Lundquist, John E.] US Forest Serv, Anchorage, AK 99501 USA. RP Lundquist, JE (reprint author), US Forest Serv, Anchorage, AK 99501 USA. EM jlundquist@fs.fed.us FU USDA National Institute of Food and Agriculture, McIntire-Stennis FX This work was supported by the USDA National Institute of Food and Agriculture, McIntire-Stennis. NR 57 TC 0 Z9 0 U1 7 U2 7 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD OCT PY 2016 VL 27 IS 5 BP 1177 EP 1189 DI 10.1007/s11676-016-0234-1 PG 13 WC Forestry SC Forestry GA EA1MX UT WOS:000386357500023 ER PT J AU Tao, J Wu, D Gourley, J Zhang, SQ Crow, W Peters-Lidard, C Barros, AP AF Tao, Jing Wu, Di Gourley, Jonathan Zhang, Sara Q. Crow, Wade Peters-Lidard, Christa Barros, Ana P. TI Operational hydrological forecasting during the IPHEx-IOP campaign - Meet the challenge SO JOURNAL OF HYDROLOGY LA English DT Article DE Integrated Precipitation and Hydrology Experiment (IPHEx); Operational flood forecasts; Quantitative Precipitation Estimate (QPE); Quantitative Precipitation Forecast (QPF); Data assimilation; Duke Coupled surface-groundwater; Hydrology Model (DCHM) ID OBSERVING SYSTEM SIMULATION; ENSEMBLE PREDICTION SYSTEMS; SNOW MICROWAVE EMISSIONS; DATA ASSIMILATION SCHEME; FLOOD ALERT SYSTEM; MEDIUM-SIZE BASIN; X-BAND RADAR; MAP D-PHASE; FLASH-FLOOD; SOIL-MOISTURE AB An operational streamflow forecasting testbed was implemented during the Intense Observing Period (IOP) of the Integrated Precipitation and Hydrology Experiment (IPHEx-IOP) in May-June 2014 to characterize flood predictability in complex terrain. Specifically, hydrological forecasts were issued daily for 12 headwater catchments in the Southern Appalachians using the Duke Coupled surface-groundwater Hydrology Model (DCHM) forced by hourly atmospheric fields and QPFs (Quantitative Precipitation Forecasts) produced by the NASA-Unified Weather Research and Forecasting (NU-WRF) model. Previous day hindcasts forced by radar-based QPEs (Quantitative Precipitation Estimates) were used to provide initial conditions for present day forecasts. This manuscript first describes the operational testbed framework and workflow during the IPHEx-IOP including a synthesis of results. Second, various data assimilation approaches are explored a posteriori (post-IOP) to improve operational (flash) flood forecasting. Although all flood events during the MP were predicted by the IPHEx operational testbed with lead times of up to 6 h, significant errors of over-and, or under-prediction were identified that could be traced back to the QPFs and subgrid-scale variability of radar QPEs. To improve operational flood prediction, three data-merging strategies were pursued post-IOP: (1) the spatial patterns of QPFs were improved through assimilation of satellite-based microwave radiances into NU-WRF; (2) QPEs were improved by merging raingauge observations with ground-based radar observations using bias correction methods to produce streamflow hindcasts and associated uncertainty envelope capturing the streamflow observations, and (3) river discharge observations were assimilated into the DCHM to improve streamflow forecasts using the Ensemble Kalman Filter (EnKF), the fixed-lag Ensemble Kalman Smoother (EnKS), and the Asynchronous EnKF (i.e. AEnKF) methods. Both flood hindcasts and forecasts were significantly improved by assimilating discharge observations into the DCHM. Specifically, Nash-Sutcliff Efficiency (NSE) values as high as 0.98, 0.71 and 0.99 at 15-min time-scales were attained for three headwater catchments in the inner mountain region demonstrating that the assimilation of discharge observations at the basin's outlet can reduce the errors and uncertainties in soil moisture at very small scales. Success in operational flood forecasting at lead times of 6, 9, 12 and 15 h was also achieved through discharge assimilation with NSEs of 0.87, 0.78, 0.72 and 0.51, respectively. Analysis of experiments using various data assimilation system configurations indicates that the optimal assimilation time window depends both on basin properties and storm-specific space-time-structure of rainfall, and therefore adaptive, context-aware configurations of the data assimilation system are recommended to address the challenges of flood prediction in headwater basins. (C) 2016 Elsevier B.V. All rights reserved. C1 [Tao, Jing; Barros, Ana P.] Duke Univ, Dept Civil & Environm Engn, Durham, NC 27706 USA. [Peters-Lidard, Christa] NASA, GSFC, Hydrol Sci Lab, Greenbelt, MD USA. [Gourley, Jonathan] NOAA, Natl Severe Storms Lab, Norman, OK 73069 USA. [Wu, Di; Zhang, Sara Q.] NASA, GSFC, Mesoscale Atmospher Proc Lab, Greenbelt, MD USA. [Wu, Di; Zhang, Sara Q.] SAIC, Mclean, VA USA. [Crow, Wade] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Barros, AP (reprint author), Duke Univ, Dept Civil & Environm Engn, Durham, NC 27706 USA. EM barros@duke.edu RI Peters-Lidard, Christa/E-1429-2012; Barros, Ana/A-3562-2011 OI Peters-Lidard, Christa/0000-0003-1255-2876; Barros, Ana/0000-0003-4606-3106 FU NASA's Precipitation Measurement Missions Program; GPM Ground Validation [NNX14AE71G] FX This work was supported by NASA's Precipitation Measurement Missions Program and GPM Ground Validation (Grant Number NNX14AE71G with Ana Barros, the corresponding author). The first author was a Ph.D. student at Duke University in the Barros group, and now is at the Earth System Science Interdisciplinary Center (ESSIC) of the University of Maryland. Barros group members Miguel Nogueira, Lauren Lowman, and Yajuan (Viola) Duan downloaded precipitation and discharge data, and helped with down scaling precipitation and analysis during the IPHEx-IOP. Di Wu and Christa Peters-Lidard provided the NU-WRF forecasting fields including QPFs for the entire IOP. Sara Zhang conducted the NU-WRF data assimilation simulations for the May 15th event. Jonathan Gourley facilitated access to the Q3 data and provided the NOXP QPEs. We thank Manos Anagnostou's group at University of Connecticut for their valuable input, and all participants in the 'GPM-GV Real-time IPHEx Hydrological Modeling Email List' for their participation. NR 147 TC 1 Z9 1 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD OCT PY 2016 VL 541 SI SI BP 434 EP 456 DI 10.1016/j.jhydrol.2016.02.019 PN A PG 23 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EA2KM UT WOS:000386421200033 ER PT J AU Wagenbrenner, JW Robichaud, PR Brown, RE AF Wagenbrenner, J. W. Robichaud, P. R. Brown, R. E. TI Rill erosion in burned and salvage logged western montane forests: Effects of logging equipment type, traffic level, and slash treatment SO JOURNAL OF HYDROLOGY LA English DT Article DE Salvage logging; Wildfire; Runoff; Soil compaction; Soil water repellency; Sediment ID SOIL PHYSICAL-PROPERTIES; COLORADO FRONT RANGE; POSTFIRE MANAGEMENT; VEGETATION RECOVERY; SEDIMENT TRANSPORT; PRESCRIBED-FIRE; ERODING RILLS; UNITED-STATES; WILDFIRE; HILLSLOPE AB Following wildfires, forest managers often consider salvage logging burned trees to recover monetary value of timber, reduce fuel loads, or to meet other objectives.. Relatively little is known about the cumulative hydrologic effects of wildfire and subsequent timber harvest using logging equipment. We used controlled rill experiments in logged and unlogged (control) forests burned at high severity in northern Montana, eastern Washington, and southern British Columbia to quantify rill overland flow and sediment production rates (fluxes) after ground-based salvage logging. We tested different types of logging equipment feller-bunchers, tracked and wheeled skidders, and wheeled forwarders as well as traffic levels and the addition of slash to skid trails as a best management practice. Rill experiments were done at each location in the first year after the fire and repeated in subsequent years. Logging was completed in the first or second post-fire year. We found that ground-based logging using heavy equipment compacted soil, reduced soil water repellency, and reduced vegetation cover. Vegetation recovery rates were slower in most logged areas than the controls. Runoff rates were higher in the skidder and forwarder plots than their respective controls in the Montana and Washington sites in the year that logging occurred, and the difference in runoff between the skidder and control plots at the British Columbia site was nearly significant (p = 0.089). Most of the significant increases in runoff in the logged plots persisted for subsequent years. The type of skidder, the addition of slash, and the amount of forwarder traffic did not significantly affect the runoff rates. Across the three sites, rill sediment fluxes were 5-1900% greater in logged plots than the controls in the year of logging, and the increases were significant for all logging treatments except the low use forwarder trails. There was no difference in the first-year sediment fluxes between the feller-buncher and tracked skidder plots, but the feller-buncher fluxes were lower than the values froth the wheeled skidder plots. Manually adding slash after logging did not affect sediment flux rates. There were no significant changes in the control sediment fluxes over time, and none of the logging equipment impacted plots produced greater sediment fluxes than the controls in the second or third year after logging. Our results indicate that salvage logging increases the risk of sedimentation regardless of equipment type and amount of traffic, and that specific best management practices are needed to mitigate the hydrologic impacts of post-fire salvage logging. (C) 2016 Elsevier B.V. All rights reserved. C1 [Wagenbrenner, J. W.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. [Robichaud, P. R.; Brown, R. E.] US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, 1221 S Main St, Moscow, ID 83843 USA. RP Wagenbrenner, JW (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. EM jwwagenb@mtu.edu FU Joint Fire Science Program [JFSP 06-3-4-21] FX This work was funded in part by the Joint Fire Science Program under Project JFSP 06-3-4-21. We thank the Moscow Forestry Sciences Laboratory personnel for their help in the field and laboratory and Lee MacDonald, Abby Corte, Ryan Lockwood, Maruxa Malvar, Sujung Ahn, Holly Brown, Patrick Robichaud, and Victoria Balfour for their assistance in field data collection. We also thank Louise Ashmun and Sarah Lewis for their input and reviews of an early draft of the manuscript and Sierra Larson and Sarah Lewis for preparing Fig. 1. Two anonymous reviewers and the editor provided suggestions for improving the paper and we appreciate their contributions. NR 82 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD OCT PY 2016 VL 541 BP 889 EP 901 DI 10.1016/j.jhydrol.2016.07.049 PN B PG 13 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EA2GI UT WOS:000386410400018 ER PT J AU Sharifi, A Lang, MW McCarty, GW Sadeghi, AM Lee, S Yen, H Rabenhorst, MC Jeong, J Yeo, IY AF Sharifi, Arnirreza Lang, Megan W. McCarty, Gregory W. Sadeghi, Ali M. Lee, Sangchul Yen, Haw Rabenhorst, Martin C. Jeong, Jaehak Yeo, In-Young TI Improving model prediction reliability through enhanced representation of wetland soil processes and constrained model auto calibration - A paired watershed study SO JOURNAL OF HYDROLOGY LA English DT Article DE Paired watersheds; Denitrification; SWAT-PMCT; Choptank River watershed; Prior-converted cropland; Hydric soils; Historical wetlands ID COASTAL-PLAIN; FORESTED WETLANDS; CHESAPEAKE BAY; SWAT MODEL; LAND-USE; QUALITY; NITRATE; FATE; NUTRIENT; GROUNDWATER AB Process based, distributed watershed models possess a large number of parameters that are not directly measured in field and need to be calibrated, in most cases through matching modeled in-stream fluxes with monitored data. Recently, concern has been raised regarding the reliability of this common calibration practice, because models that are deemed to be adequately calibrated based on commonly used metrics (e.g., Nash Sutcliffe efficiency) may not realistically represent intra-watershed responses or fluxes. Such shortcomings stem from the use of an evaluation criteria that only concerns the global in-stream responses of the model without investigating intra-watershed responses. In this study, we introduce a modification to the Soil and Water Assessment Tool (SWAT) model, and a new calibration technique that collectively reduce the chance of misrepresenting intra-watershed responses. The SWAT model was modified to better represent NO3 cycling in soils with various degrees of water holding capacity. The new calibration tool has the capacity to calibrate paired watersheds simultaneously within a single framework. It Was found that when both proposed methodologies were applied jointly to two paired watersheds on the Delmarva Peninsula, the performance of the models as judged based on conventional metrics suffered, however, the intra-watershed responses (e.g., mass of NO3 lost to denitrification) in the two models automatically converged to realistic sums. This approach also demonstrates the capacity to spatially distinguish areas of high denitrification potential, an ability that has implications for improved management of prior converted wetlands under crop production and for identifying prominent areas for wetland restoration. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sharifi, Arnirreza] Univ Maryland, Dept Environm Sci & Technol, 10300 Baltimore Ave BLDG 007,RM 104,BARC WEST, Beltsville, MD 20705 USA. [Lang, Megan W.] USFW, Natl Wetland Inventory, Branch Geospatial Mapping & Tech Support, 5275 Leesburg Pike, Falls Church, VA 22041 USA. [Lee, Sangchul] Univ Maryland, Dept Geog Sci, 10300 Baltithore Ave BLDG 007,RM 104,BARC WEST, Beltsville, MD 20705 USA. [McCarty, Gregory W.; Sadeghi, Ali M.] USDA ARS, Hydrol & Remote Sensing Lab, 10300 Baltitore Ave BLDG 007,RM 104,BARC WEST, Beltsville, MD 20705 USA. [Yen, Haw; Jeong, Jaehak] Texas A&M Univ, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. [Rabenhorst, Martin C.] Univ Maryland, Dept Environm Sci & Technol, 0208 HJ Patterson Bldg, College Pk, MD 20742 USA. [Yeo, In-Young] Univ Newcastle, Sch Engn, EA 127,Univ Dr, Callaghan, NSW 2308, Australia. RP Sharifi, A (reprint author), Univ Maryland, Dept Environm Sci & Technol, 10300 Baltimore Ave BLDG 007,RM 104,BARC WEST, Beltsville, MD 20705 USA. EM sharifi@umd.edu FU United States Department of Agriculture (USDA) Natural Resources Conservation Service (NRCS) Conservation Effects Assessment Project (CEAP) Wetland and Watershed Components FX This project was funded by the United States Department of Agriculture (USDA) Natural Resources Conservation Service (NRCS) Conservation Effects Assessment Project (CEAP) Wetland and Watershed Components. The authors would like to thank Mari-Vaughn Johnson and William Effland, USDA NRCS, for their support and constructive review of the manuscript. NR 55 TC 2 Z9 2 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD OCT PY 2016 VL 541 BP 1088 EP 1103 DI 10.1016/j.jhydrol.2016.08.022 PN B PG 16 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EA2GI UT WOS:000386410400034 ER PT J AU Lillehoj, HS Lee, SH Park, SS Jeong, M Lim, Y Mathis, GF Lumpkins, B Chi, F Ching, C Cravens, RL AF Lillehoj, Hyun S. Lee, Sung H. Park, Soon S. Jeong, Misun Lim, Yeaseul Mathis, Greg F. Lumpkins, Brett Chi, Fang Ching, Chris Cravens, Ron L. TI Calcium Montmorillonite-Based Dietary Supplement Attenuates Necrotic Enteritis Induced by Eimeria maxima and Clostridium perfringens in Broilers SO JOURNAL OF POULTRY SCIENCE LA English DT Article DE calcium montmorillonite; Clostridium perfringens; coccidiosis; Eimeria maxima; necrotic enteritis; organic acid ID GROWTH-PERFORMANCE; IMMUNE-RESPONSE; ALPHA-TOXIN; CHICKENS; INFECTION; PROTECTION; ANTIBODIES; RESISTANCE; CHALLENGE; LESIONS AB Necrotic enteritis (NE) is a poultry disease caused by Clostridium perfringens and characterized by severe intestinal necrosis. The incidence of avian NE has been progressively increasing following the removal of antibiotics from poultry feed. We evaluated the effect of diets supplemented with the thermally-processed clays, calcium montmorillonite (CaMM) on clinical signs, immunopathology, and cytokine responses in broiler chickens using an experimental model of NE consisting of co-infection with Eimeria maxima and C. perfringens. In Trial 1, Ross/Ross chickens were fed from hatch with a normal basal diet or a CaMM-supplemented diet with or without a fermentable fiber, an organic acid, and/or a plant extract, and co-infected with E. maxima and C. perfringens under conditions simulating clinical infection in the field. Chickens fed a diet supplemented with CaMM plus a fermentable fiber and an organic acid had increased body weight gain, reduced gut lesions, and increased serum antibody levels to C. perfringens a-toxin and NetB toxin compared with chickens fed the basal diet alone. Levels of transcripts for interleukin-1 beta (IL-1 beta), IL-6, inducible nitric oxide synthase, and tumor necrosis factor-a superfamily-15 were significantly altered in the intestine and spleen of CaMM-supplemented chickens compared with unsupplemented controls (p<0.05). In Trial 2, Cobb/Cobb chickens were fed an unsupplemented diet or a diet supplemented with CaMM or Varium, each with a fermentable fiber and an organic acid, and co-infected with E. maxima and C. perfringens under subclinical infection conditions. Compared with unsupplemented controls, broilers fed with CaMM plus a fermentable fiber and an organic acid had increased body weight gain, and reduced feed conversion ratio, mortality, and intestinal lesions, compared with chickens fed an unsupplemented diet (p<0.05). Dietary supplementation of broiler chickens with CaMM plus a fermentable fiber and an organic acid might be useful to control avian NE in the field. C1 [Lillehoj, Hyun S.; Lee, Sung H.; Park, Soon S.; Jeong, Misun; Lim, Yeaseul] ARS, Anim Biosci & Biotechnol Lab, USDA, Bldg 1043,BARC East, Beltsville, MD 20705 USA. [Mathis, Greg F.; Lumpkins, Brett] Southern Poultry Res Inc, Athens, GA 30607 USA. [Chi, Fang; Ching, Chris; Cravens, Ron L.] Amlan Int, Chicago, IL 60611 USA. [Lee, Sung H.] Natl Acad Agr Sci, Rural Dev Adm, Wonju 565851, Jeollabuk Do, South Korea. RP Lillehoj, HS (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Bldg 1043,BARC East, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS; USDA; USA; Rural Development Administration, Republic of Korea [PJ 012088, PJ01049004] FX The authors express their appreciation to Chuck Lowe for assistance, and to Dr. Erik P. Lillehoj for critical review.This study was partially supported by a formal Trust agreement established between ARS, USDA, USA and the Rural Development Administration, Republic of Korea (No. PJ 012088, PJ01049004). NR 36 TC 0 Z9 0 U1 12 U2 12 PU JAPAN POULTRY SCIENCE ASSOC PI TSUKUBA PA C/O NATL INST LIVESTOCK & GLASSLAND SCIENCE, 2 IKENODAI, TSUKUBA, IBARAKI 305-0901, JAPAN SN 1346-7395 J9 J POULT SCI JI J. Poult. Sci. PD OCT PY 2016 VL 53 IS 4 BP 329 EP 340 DI 10.2141/jpsa.0150182 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA EA2LF UT WOS:000386423100011 ER PT J AU Johnson, JS Martin, KL Pohler, KG Stewart, KR AF Johnson, J. S. Martin, K. L. Pohler, K. G. Stewart, K. R. TI Effects of rapid temperature fluctuations prior to breeding on reproductive efficiency in replacement gilts SO JOURNAL OF THERMAL BIOLOGY LA English DT Article DE Breeding; Fetal viability; Pigs; Rapid cooling; Temperature fluctuations ID NECROSIS-FACTOR-ALPHA; HEAT-STRESS; INSULIN-RESISTANCE; TNF-ALPHA; SEASONAL INFERTILITY; INTESTINAL PERMEABILITY; BODY-TEMPERATURE; OVARIAN-FUNCTION; SOWS; PREGNANCY AB Rapidly cooling pigs after heat stress (HS) results in a pathophysiological condition, and because rapid temperature fluctuations may be associated with reduced reproductive success in sows, it lends itself to the hypothesis that these conditions may be linked. Objectives were to determine the effects of rapid cooling on thermal response and future reproductive success in pigs. Thirty-six replacement gifts (137.8 +/- 0.9 kg BW) were estrus synchronized and then 14.1 +/- 0.4 d after estrus confirmation, pigs were exposed to thermoneutral conditions (TN; n=12; 19.7 +/- 0.9 degrees C) for 6 h, or HS (36.3 +/- 0.5 degrees C) for 3 h, followed by 3 h of rapid cooling (HSRC; n=12; immediate TN exposure and water dousing) or gradual cooling (HSGC; n=12; gradual decrease to TN conditions) repeated over 2 d. Vaginal (T-V) and gastrointestinal tract temperatures (T-GI) were obtained every 15 min, and blood was collected on d 1 and d 2 during the HS and recovery periods at 180 and 60 min, respectively. Pigs were bred 8.3 +/- 0.8 d after thermal treatments over 2 d. Reproductive tracts were collected and total fetus number and viability were recorded 28.0 +/- 0.8 d after insemination. HS increased T-V and T-GI (P=0.01; 0.98 degrees C) in HSRC and HSGC compared to TN pigs. During recovery, T-V was reduced from 15 to 105 min (P=0.01; 033 degrees C) in HSRC compared to HSGC pigs, but no overall differences in T-GI were detected (P<0.05; 39.67 degrees C). Rapid cooling increased (P<0.05) TNF alpha compared to HSGC and TN pigs during recovery-d 1 (55.2%), HS-d 2 (35.1%), and recovery-d 2 (64.9%). Viable fetuses tended to be reduced (P=0.08; 10.5%) and moribund fetuses tended to be increased (P=0.09; 159.3%) in HSRC compared to HSGC and TN pigs. In summary, rapid cooling prior to breeding may contribute to reduced fetal viability and reproductive success in pigs. Published by Elsevier Ltd. C1 [Johnson, J. S.] ARS, USDA, Livestock Behav Res Unit, 125 S Russell St,Rm 219C, W Lafayette, IN 47907 USA. [Martin, K. L.; Stewart, K. R.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Pohler, K. G.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. RP Johnson, JS (reprint author), ARS, USDA, Livestock Behav Res Unit, 125 S Russell St,Rm 219C, W Lafayette, IN 47907 USA. EM Jay.Johnson@ars.usda.gov NR 53 TC 1 Z9 1 U1 2 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4565 J9 J THERM BIOL JI J. Therm. Biol. PD OCT PY 2016 VL 61 BP 29 EP 37 DI 10.1016/j.jtherbio.2016.08.003 PG 9 WC Biology; Zoology SC Life Sciences & Biomedicine - Other Topics; Zoology GA EA2FR UT WOS:000386408700004 PM 27712657 ER PT J AU Shea, MK Loeser, RF Hsu, FC Booth, SL Nevitt, M Simonsick, EM Strotmeyer, ES Vermeer, C Kritchevsky, SB AF Shea, M. Kyla Loeser, Richard F. Hsu, Fang-Chi Booth, Sarah L. Nevitt, Michael Simonsick, Eleanor M. Strotmeyer, Elsa S. Vermeer, Cees Kritchevsky, Stephen B. CA Hlth ABC Study TI Vitamin K Status and Lower Extremity Function in Older Adults: The Health Aging and Body Composition Study SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Physical performance; Nutrition; Physical function; Vitamin K ID MATRIX GLA PROTEIN; PHYSICAL PERFORMANCE; KNEE OSTEOARTHRITIS; MOBILITY DISABILITY; MEANINGFUL CHANGE; CLINICAL-TRIAL; ASSOCIATION; CALCIFICATION; WOMEN; INFLAMMATION AB While low vitamin K status has been associated with several chronic diseases that can lead to lower extremity disability, it is not known if low vitamin K status is associated with worse lower extremity function. Vitamin K status was measured according to plasma phylloquinone (vitamin K1) and dephosphorylated-uncarboxylated MGP (dp-ucMGP) in 1,089 community-dwelling older adults (mean +/- SD age =74 +/- 3 years; 67% female). Lower extremity function was assessed using the short physical performance battery (SPPB), gait speed, and isokinetic leg strength. Linear regression and mixed models were used to determine the cross-sectional and longitudinal associations between vitamin K status and functional outcome measures. Cross-sectionally, higher plasma phylloquinone was associated with better SPPB scores and 20-m gait speed (p a parts per thousand currency sign .05). After 4-5 years, those with a parts per thousand yen1.0nM plasma phylloquinone (the concentration achieved when recommended intakes are met) had better SPPB scores (p = .03) and 20-m gait speed (p < .05). Lower plasma dp-ucMGP (reflective of better vitamin K status) was associated with better SPPB scores and leg strength cross-sectionally (p a parts per thousand currency sign .04), but not longitudinally. Neither measure of vitamin K status was associated with walking endurance or with the rate of decline in function. Older adults with higher vitamin K status had better physical performance scores at baseline, but data are less consistent longitudinally. Since lower extremity disability is a common consequence of multiple chronic diseases for which a role of vitamin K has been suggested, future studies are needed to determine if vitamin K supplementation could improve function in those with vitamin K insufficiency and clarify underlying mechanism(s). C1 [Shea, M. Kyla; Booth, Sarah L.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Loeser, Richard F.] Univ N Carolina, Div Rheumatol Allergy & Immunol, Chapel Hill, NC USA. [Hsu, Fang-Chi] Wake Forest Sch Med, Div Publ Hlth Sci, Winston Salem, NC USA. [Nevitt, Michael] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. [Simonsick, Eleanor M.] NIA, NIH, Baltimore, MD 21224 USA. [Strotmeyer, Elsa S.] Univ Pittsburgh, Grad Sch Publ Hlth, Pittsburgh, PA 15260 USA. [Vermeer, Cees] Maastricht Univ, VitaK, NL-6200 MD Maastricht, Netherlands. [Kritchevsky, Stephen B.] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. RP Shea, MK (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM kyla.shea@tufts.edu FU NIH Intramural Research Program; NIA; NINR [R01-NR012459]; NIAMS [R21AR062284, K01AR063167]; Arthritis Foundation New Investigator Grant; USDA, Agricultural Research Service Cooperative Agreement [58-1950-7-707]; [N01-AG-6-2101]; [N01-AG-6-2103]; [N01-AG-6-2106]; [R01-AG028050] FX This study was supported by the NIH Intramural Research Program, the NIA and contracts (N01-AG-6-2101, N01-AG-6-2103, and N01-AG-6-2106; R01-AG028050); the NINR (R01-NR012459); the NIAMS (R21AR062284, K01AR063167); an Arthritis Foundation New Investigator Grant and the USDA, Agricultural Research Service Cooperative Agreement (58-1950-7-707). NR 30 TC 1 Z9 1 U1 1 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD OCT PY 2016 VL 71 IS 10 BP 1348 EP 1355 DI 10.1093/gerona/glv209 PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DZ7WI UT WOS:000386078800014 PM 26576842 ER PT J AU Harlow, BE Bryant, RW Cohen, SD O'Connell, SP Flythe, MD AF Harlow, B. E. Bryant, R. W. Cohen, S. D. O'Connell, S. P. Flythe, M. D. TI Degradation of spent craft brewer's yeast by caprine rumen hyper ammonia-producing bacteria SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE brewer's yeast; bypass protein; goat; hops; micro-organism; phyto-ionophore; ruminant ID ACID; HOPS; LUPULONE AB Spent yeast from craft beers often includes more hops (Humulus lupulus L.) secondary metabolites than traditional recipes. These compounds include alpha- and beta-acids, which are antimicrobial to the rumen hyper ammonia-producing bacteria (HAB) that are major contributors to amino acid degradation. The objective was to determine if the hops acids in spent craft brewer's yeast (CY; similar to 3.5 mg g(-1) hops acids) would protect it from degradation by caprine rumen bacteria and HAB when compared to a baker's yeast (BY; no hops acids). Cell suspensions were prepared by harvesting rumen fluid from fistulated goats, straining and differential centrifugation. The cells were re-suspended in media with BY or CY. After 24 h (39 degrees C), HAB were enumerated and ammonia was measured. Fewer HAB and less ammonia was produced from CY than from BY. Pure culture experiments were conducted with Peptostreptococcus anaerobius BG1 (caprine HAB). Ammonia production by BG1 from BY was greater than from CY. Ammonia production was greater when exogenous amino acids were included, but similar inhibition was observed in CY treatments. These results indicate that rumen micro-organisms deaminated the amino acids in CY to a lesser degree than BY. C1 [Harlow, B. E.; Flythe, M. D.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY USA. [Bryant, R. W.] Asheville Flavor Innovat LLC, Asheville, NC USA. [Cohen, S. D.] Appalachian State Univ, Fermentat Sci, Boone, NC 28608 USA. [O'Connell, S. P.] Western Carolina Univ, Dept Biol, Cullowhee, NC 28723 USA. [Flythe, M. D.] ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY USA. RP Flythe, MD (reprint author), Univ Kentucky, USDA, ARS, N-220 Ag Sci North, Lexington, KY 40546 USA. EM michael.flythe@ars.usda.gov FU USDA-ARS National Program, Food-Animal Production [101] FX The project was funded by USDA-ARS National Program 101, Food-Animal Production. The 'Beta Bio' hops extract was donated by the manufacturer S. S. Steiner, Inc. Spent brewer's yeast slurry was donated by Highland Brewing Company, Asheville, NC. NR 22 TC 1 Z9 1 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD OCT PY 2016 VL 63 IS 4 BP 307 EP 312 DI 10.1111/lam.12623 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DZ7DB UT WOS:000386023300011 PM 27471059 ER PT J AU Tidwell, TL AF Tidwell, Thomas L. TI Introduction by USFS Chief Tidwell - Pollinators and Pollination SO NATURAL AREAS JOURNAL LA English DT Editorial Material C1 [Tidwell, Thomas L.] US Forest Serv, Washington, DC 20250 USA. RP Tidwell, TL (reprint author), US Forest Serv, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 361 EP 361 PG 1 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300002 ER PT J AU Cane, JH Love, B AF Cane, James H. Love, Byron TI Floral Guilds of Bees in Sagebrush Steppe: Comparing Bee Usage of Wildflowers Available for Postfire Restoration SO NATURAL AREAS JOURNAL LA English DT Article DE Apoidea; Asteraceae; Great Basin; oligolecty; restoration ID SAGE GROUSE; ASTRAGALUS; ATTRACTIVENESS; MEGACHILIDAE; HYMENOPTERA; PATTERNS; BIOLOGY AB Healthy plant communities of the American sagebrush steppe consist of mostly wind-pollinated shrubs and grasses interspersed with a diverse mix of mostly spring-blooming, herbaceous perennial wildflowers. Native, nonsocial bees are their common floral visitors, but their floral associations and abundances are poorly known. Extrapolating from the few available pollination studies, bees are the primary pollinators needed for seed production. Bees, therefore, will underpin the success of ambitious seeding efforts to restore native forbs to impoverished sagebrush steppe communities following vast wildfires. This study quantitatively characterized the floral guilds of 17 prevalent wildflower species of the Great Basin that are, or could be, available for restoration seed mixes. More than 3800 bees representing >170 species were sampled from >35,000 plants. Species of Osmia, Andrena, Bombus, Eucera, Halictus, and Lasioglossum bees prevailed. The most thoroughly collected floral guilds, at Balsamorhiza sagittata and Astragalus filipes, comprised 76 and 85 native bee species, respectively. Pollen-specialists dominated guilds at Lomatium dissectum, Penstemon speciosus, and several congenerics. In contrast, the two native wildflowers used most often in sagebrush steppe seeding mixes-Achillea millefolium and Linum lewisii-attracted the fewest bees, most of them unimportant in the other floral guilds. Successfully seeding more of the other wildflowers studied here would greatly improve degraded sagebrush steppe for its diverse native bee communities. C1 [Cane, James H.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84341 USA. [Love, Byron] Utah State Univ, Dept Biol, Logan, UT 84341 USA. RP Cane, JH (reprint author), Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84341 USA. EM jim.cane@usu.edu FU USDA Forest Service; Rocky Mountain Research Station; Great Basin Native Plant Selection and Increase Project; USDI Bureau of Land Management, Great Basin Restoration Initiative FX This research was largely funded by the USDA Forest Service, Rocky Mountain Research Station, Great Basin Native Plant Selection and Increase Project, and the USDI Bureau of Land Management, Great Basin Restoration Initiative. We are grateful to Terry Griswold for his taxonomic assistance with several of the more difficult bee genera, and to Kristal Watrous, Linda Kervin, and Seth Nothsinger for several of the collections. We also appreciate the perennial interest, encouragement, and insights offered by Nancy Shaw (USFS). We dedicate this manuscript to the memory of Dr. Charles Michener, mentor to JHC. NR 34 TC 1 Z9 1 U1 18 U2 18 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 377 EP 391 PG 15 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300005 ER PT J AU Hanula, JL Ulyshen, MD Horn, S AF Hanula, James L. Ulyshen, Michael D. Horn, Scott TI Conserving Pollinators in North American Forests: A Review SO NATURAL AREAS JOURNAL LA English DT Review DE fire; forest management; invasive species; prescribed burning; verges ID NATIVE PLANT-COMMUNITIES; SHRUB LONICERA-MAACKII; CHEATGRASS BROMUS-TECTORUM; TRIN. A. CAMUS; RIPARIAN FORESTS; INVASIVE SHRUB; UNITED-STATES; JAPANESE STILTGRASS; CHINESE PRIVET; ROAD VERGES AB Bees and butterflies generally favor open forest habitats regardless of forest type, geographic region, or methods used to create these habitats. Dense shrub layers of native or nonnative species beneath forest canopies negatively impact herbaceous plant cover and diversity, and pollinators. The presence of nonnative flowers as a source of nectar, pollen, or larval food can have positive or negative effects on pollinators depending on the situation, but in cases where the nonnatives exclude native plants, the results are almost always negative. Roads and roadside corridors offer an opportunity to increase open, pollinator-friendly habitat even in dense forests by thinning the adjacent forest, mowing at appropriate times, and converting to native herbaceous plant communities where nonnative species have been planted or have invaded. Efforts to improve forest conditions for pollinators should consider the needs of specialist species and vulnerable species with small scattered populations. Conservation of bees and butterflies, as well as other pollinating species, in forested areas is important for most forest plant species, and forests may serve as reservoirs of pollinators for recolonization of surrounding habitats. C1 [Hanula, James L.; Ulyshen, Michael D.; Horn, Scott] US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM mulyshen@fs.us NR 172 TC 3 Z9 3 U1 30 U2 30 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 427 EP 439 PG 13 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300009 ER PT J AU DeBano, SJ Roof, SM Rowland, MM Smith, LA AF DeBano, Sandra J. Roof, Samantha M. Rowland, Mary M. Smith, Lauren A. TI Diet Overlap of Mammalian Herbivores and Native Bees: Implications for Managing Co-occurring Grazers and Pollinators SO NATURAL AREAS JOURNAL LA English DT Article DE dietary overlap; livestock; native bees; Starkey Experimental Forest and Range; wild ungulates ID ROCKY-MOUNTAIN ELK; MULE DEER DIETS; NORTHEASTERN OREGON; CATTLE DIETS; GRAZING INTENSITY; BOTANICAL COMPOSITION; BUNCHGRASS PRAIRIE; ECOSYSTEM SERVICES; BLUE MOUNTAINS; NESTING BEES AB Many federal, state, and tribal agencies, as well as nonprofit organizations, have recently increased efforts to understand how natural areas can be managed to enhance native pollinators and the ecosystem services they provide. However, managing this important group must be balanced with other services that natural areas provide including hunting, timber production, and livestock grazing. Significant knowledge gaps exist about how to effectively manage habitats used by large ungulates (e.g., cattle (Bos taurus), elk (Cervus elaphus), mule deer (Odocoileus hemionus)) in ways that also enhance pollinators. One key gap is understanding the degree to which diets of mammalian herbivores overlap with floral resources used by bees, and how this overlap varies spatially and temporally. Invertebrate pollinators, including bees, rely on flowering forbs and shrubs for nectar and pollen. Ungulates also feed on flowering plants, although preferences vary by ungulate species, vegetation community, and season. Here we review existing literature on ungulate diets relative to flowering plants and compare this information with flower preferences of bees, drawing on studies of bee abundance and diversity at the Starkey Experimental Forest and Range in northeastern Oregon. Our review can inform managers about the potential dietary overlap between ungulates and native bees and aid planning efforts aimed at biodiversity conservation of pollinators. We discuss management implications relative to seasonal habitat use and dietary preferences of ungulates and variation in bee phenology, and conclude with guidance about timing and intensity of ungulate grazing when managing for multiple conservation objectives, especially in sensitive habitats like riparian areas. C1 [DeBano, Sandra J.; Smith, Lauren A.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Dept Fisheries & Wildlife, Hermiston, OR 97838 USA. [Roof, Samantha M.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Bioresource Res Program, Hermiston, OR 97838 USA. [Rowland, Mary M.] US Forest Serv, USDA, Pacific Northwest Res Stn, La Grande Forestry & Range Sci Lab, La Grande, OR 97850 USA. RP DeBano, SJ (reprint author), Oregon State Univ, Hermiston Agr Res & Extens Ctr, Dept Fisheries & Wildlife, Hermiston, OR 97838 USA. EM sandy.debano@oregonstate.edu FU USDA Forest Service, Pacific Northwest Research Station; Oregon State University Provost's Branch Experiment Station Experiential Learning Program; Northwest Scientific Association; USDA National Needs Fellowship from the National Institute of Food and Agriculture FX Funding for this project was provided by the USDA Forest Service, Pacific Northwest Research Station. S. Roof received funding from Oregon State University Provost's Branch Experiment Station Experiential Learning Program and a student grant from the Northwest Scientific Association. L. Smith was supported by a USDA National Needs Fellowship from the National Institute of Food and Agriculture. We thank K. Coe for his help in identifying plant species and S. Clark and S. Burrows for help in identifying bees. B. Dick, R. Kennedy, and D. Rea assisted with logistics for housing and other field support. The comments of two anonymous reviewers improved this manuscript. NR 130 TC 1 Z9 1 U1 17 U2 17 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 458 EP 477 PG 20 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300012 ER PT J AU Aslan, CE Liang, CT Galindo, B Hill, K Topete, W AF Aslan, Clare E. Liang, Christina T. Galindo, Ben Hill, Kimberly Topete, Walter TI The Role of Honey Bees as Pollinators in Natural Areas SO NATURAL AREAS JOURNAL LA English DT Article DE Apis mellifera; competition; mutualistic networks; native bees; pollination ID CASHEW ANACARDIUM-OCCIDENTALE; APIS-MELLIFERA; REPRODUCTIVE SUCCESS; INTRODUCED HONEYBEES; FLORAL RESOURCES; NATIVE PLANT; CENTAUREA-SOLSTITIALIS; NORTHEASTERN BRAZIL; ECOLOGICAL IMPACTS; HYMENOPTERA APIDAE AB The western or European honey bee (Apis mellifera) is the primary managed pollinator in US agricultural systems, and its importance for food production is widely recognized. However, the role of A. mellifera as an introduced species in natural areas is potentially more complicated. The impact of A. mellifera on native insect pollinators can depend on broad community context, as can the relative effectiveness of A. mellifera in pollination of both native and nonnative plant species outside of agricultural systems. Apis mellifera is highly generalist and able to interact with hundreds of native plant species following its naturalization. It is unlikely to wholly replace native pollinators as visitors of specialized plant species, and its behavioral characteristics tend to reduce A. mellifera's per-visit efficiency, even when its overall effectiveness is high. Preliminary results of our case study exploring the importance of A. mellifera vs. native bees as pollinators of native plants in Hawai'i indicate that A. mellifera is less important than native Hylaeus bees as a flower visitor of focal native plant species. In light of current global declines in A. mellifera populations, maintenance of a diversity of pollinators and pollinator habitat are critical conservation needs in natural areas. C1 [Aslan, Clare E.] No Arizona Univ, Landscape Conservat Initiat, Flagstaff, AZ 86005 USA. [Liang, Christina T.] US Forest Serv, USDA, Pacific Southwest Res Stn, 60 Nowelo St, Hilo, HI 96720 USA. [Galindo, Ben] Southwestern Univ, Georgetown, TX USA. [Hill, Kimberly] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Topete, Walter] Univ Houston, Houston, TX USA. RP Aslan, CE (reprint author), No Arizona Univ, Landscape Conservat Initiat, Flagstaff, AZ 86005 USA. EM clare.aslan@nau.edu FU Strategic Environmental Research and Development Program; Doris Duke Conservation Scholars Program FX Funding was provided by the Strategic Environmental Research and Development Program and the Doris Duke Conservation Scholars Program. We further thank the Doris Duke Charitable Foundation and Doris Duke Conservation Scholars Program for the enormous logistical and training support provided throughout the field study. Thanks to Heather Coad and Kristen Potter for their invaluable logistical and field help. NR 120 TC 0 Z9 0 U1 72 U2 72 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 478 EP 488 PG 11 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300013 ER PT J AU Dumroese, RK Luna, T Pinto, JR Landis, TD AF Dumroese, R. Kasten Luna, Tara Pinto, Jeremiah R. Landis, Thomas D. TI Forbs: Foundation for Restoration of Monarch Butterflies, Other Pollinators, and Greater Sage-Grouse in the Western United States SO NATURAL AREAS JOURNAL LA English DT Article DE Centrocercus urophasianus; Danaus plexippus; forbs; native plants; wildflowers ID FOREST RESTORATION; SLICKSPOT PEPPERGRASS; LEPIDIUM-PAPILLIFERUM; OUACHITA MOUNTAINS; PLANT-COMMUNITIES; NORTH-AMERICA; BIG SAGEBRUSH; HABITAT USE; OREGON; CONSERVATION AB Monarch butterflies (Danaus plexippus), other pollinators, and Greater Sage-Grouse (Centrocercus urophasianus) are currently the focus of increased conservation efforts. Federal attention on these fauna is encouraging land managers to develop conservation strategies, often without corresponding financial resources. This could foster a myopic approach when allocating resources and setting restoration priorities, and at best, allow for inefficiencies in the usage of land management resources, or, at worst, pit one species (or suite of species, e.g., pollinators) against another (e.g., sage-grouse). Instead, investing holistically by linking conservation of these fauna may provide improved leverage of available resources and more benefit to the landscape. Fortunately, on the western US rangelands, these fauna can all benefit from restoration that increases the abundance and diversity of forbs. Establishing high density islands of outplanted forb seedlings may be a way to expedite restoration. Managers establishing forbs for pollinators (including monarchs) would further increase food availability for greater sage-grouse and vice versa. Adding milkweeds (Asclepias spp.) in appropriate areas to forb mixtures for restoration is warranted because they are excellent nectar sources for pollinators in general and the sole host for monarch larvae in particular. Here, we provide an overview of why forb species are keystone for monarch butterflies, other pollinators, and Greater Sage-Grouse and how seeding and outplanting seedlings of specific forbs are critical to restoration efforts. C1 [Dumroese, R. Kasten; Pinto, Jeremiah R.] US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. [Luna, Tara] POB 447, East Glacier, MT 59434 USA. [Landis, Thomas D.] Native Plant Nursery Consulting, 3248 Sycamore Way, Medford, OR 97504 USA. RP Dumroese, RK (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. EM kdumroese@fs.fed.us FU USDA Forest Service Rocky Mountain Research Station; National Center for Reforestation, Nurseries, and Genetic Resources FX This work was supported by the USDA Forest Service Rocky Mountain Research Station and the National Center for Reforestation, Nurseries, and Genetic Resources. We thank Drs. Deborah Finch, Andrew Hudak, Mee-Sook Kim, Deborah Page-Dumroese, Justin Runyon, Nancy Shaw, two anonymous reviewers, and associate editor Vicki Wojcik for manuscript reviews, and Jim Marin Graphics for line drawings. NR 89 TC 2 Z9 2 U1 30 U2 30 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD OCT PY 2016 VL 36 IS 4 BP 499 EP 511 PG 13 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DZ7NZ UT WOS:000386054300015 ER PT J AU Rothrock, MJ Locatelli, A Glenn, TC Thomas, JC Caudill, AC Kiepper, BH Hiett, KL AF Rothrock, M. J., Jr. Locatelli, A. Glenn, T. C. Thomas, J. C. Caudill, A. C. Kiepper, B. H. Hiett, K. L. TI Assessing the microbiomes of scalder and chiller tank waters throughout a typical commercial poultry processing day SO POULTRY SCIENCE LA English DT Article DE processing; microbiome; food safety; spoilage ID INTESTINAL BACTERIAL COMMUNITY; LISTERIA-MONOCYTOGENES; CAMPYLOBACTER-JEJUNI; CHICKEN MEAT; MOLECULAR CHARACTERIZATION; SALMONELLA-TYPHIMURIUM; ANTIBIOTIC-RESISTANCE; BROILER CARCASSES; FECAL MICROBIOTA; RAW POULTRY AB The commercial poultry processing environment plays a significant role in reducing foodborne pathogens and spoilage organisms from poultry products prior to being supplied to consumers. While understanding the microbiological quality of these products is essential, little is known about the microbiota of processing water tanks within the processing plant. Therefore, the goal of this study was to assess the microbiomes of the scalder and chiller tanks during a typical commercial processing d, and determine how bacterial populations, including foodborne pathogens and spoilage organisms, change during the processing day in relation to the bacterial communities as a whole. Additionally, considering this is the first microbiomic analysis of processing tank waters, 2 water sampling methods also were compared. Results of this study show that Proteobacteria and Firmicutes represented over half of the sequences recovered from both tanks at the phylum level, but the microbiomic profiles needed to be analyzed at the genus level to observe more dynamic population shifts. Bacteria known to predominate in the live production environment were found to increase in the scalder tank and gram negative spoilage-related bacteria were found to decrease in the chiller tank throughout the processing day. Directly sampling the scalder water, as compared to analyzing filtered samples, resulted in significantly different microbiomic profiles dominated by Anoxybacillus species. While no sequences related to major foodborne pathogens were found, further sampling collection and processing optimization should provide researchers and the poultry industry a new tool to understand the ecological role of spoilage and pathogenic bacteria within processing tank waters. C1 [Rothrock, M. J., Jr.; Locatelli, A.] ARS, USDA, US Natl Poultry Res Ctr, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. [Glenn, T. C.; Thomas, J. C.; Caudill, A. C.] Univ Georgia, Dept Publ Hlth, Athens, GA 30602 USA. [Kiepper, B. H.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. [Hiett, K. L.] ARS, USDA, Poultry Microbiol Safety & Proc Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Rothrock, MJ (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Egg Safety & Qual Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM Michael.rothrock@ars.usda.gov FU Agricultural Research Service, USDA CRIS [6612-41420-017-00, 6612-32000-059-00] FX The authors would like to thank J. Gregory Caporaso (Northern Arizona University) for advice on microbiomic analysis using the QIIME pipeline, as well as for performing the initial sequence analyses that were utilized as a point of comparison for the subsequent analyses described in this paper. The authors would also like to acknowledge the expert technical assistance of Latoya Wiggins, Nicole Bartenfeld, and Kathy Tate for their assistance in sampling and cultural work, as well as John Gamble and Laura Lee Rutherford for their assistance in sampling and molecular analyses. These investigations were supported equally by the Agricultural Research Service, USDA CRIS Projects "Pathogen Reduction and Processing Parameters in Poultry Processing Systems" #6612-41420-017-00, and "Molecular Approaches for the Characterization of Foodborne Pathogens in Poultry" #6612-32000-059-00. NR 69 TC 1 Z9 1 U1 5 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD OCT PY 2016 VL 95 IS 10 BP 2372 EP 2382 DI 10.3382/ps/pew234 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DZ9OF UT WOS:000386205600019 PM 27444443 ER PT J AU Chatterjee, D Zhuang, H Bowker, BC Rincon, AM Sanchez-Brambila, G AF Chatterjee, D. Zhuang, H. Bowker, B. C. Rincon, A. M. Sanchez-Brambila, G. TI Instrumental texture characteristics of broiler pectoralis major with the wooden breast condition(1) SO POULTRY SCIENCE LA English DT Article DE wooden breast condition; broiler; MORS; TPA; texture ID WARNER-BRATZLER SHEAR; INTRAMUSCULAR CONNECTIVE-TISSUE; ALLO-KRAMER SHEAR; MEAT QUALITY; COOKED MEAT; TENDERNESS; PROFILE; RAW; CHICKENS; FILLETS AB The objective was to characterize texture properties of raw and cooked broiler fillets (>Pectoralis major) with the wooden breast condition (WBC) using the instrumental texture techniques of Meullenet-Owens Razor Shear (MORS) and Texture Profile Analysis (TPA). Deboned (3 h postmortem) broiler fillets were collected from a commercial plant and categorized as normal, moderate, or severe WBC based on the incidence and severity of diffuse hardened areas throughout fillets and the degree of palpable hardness. The fillets were then either stored at 4A degrees C overnight or in a -20A degrees C freezer. The MORS and TPA of the raw samples were determined at 24 h postmortem for fresh samples and after thawing overnight for frozen samples. The same measurements were also taken after the samples were cooked to 78A degrees C. Regardless of freshness (fresh vs. frozen-thawed), cooking (raw vs. cooked), and degree of WBC, both MORS force and energy of the WBC samples were higher than that of the normal samples (>P < 0.05). For TPA adhesiveness and resilience, there were no differences between normal and WBC samples (>P > 0.05). However, average TPA hardness and chewiness measurements of the fillets with WBC were higher than the normal fillets (>P < 0.05). Regardless of texture measurement, there were no interactions between freshness and the wooden condition or no differences between moderate and severe WBC fillets (>P > 0.05). These results demonstrate that there are significant differences in instrumental texture properties between normal fillets and those exhibiting the WBC. The WBC fillets required more force to cut through, harder, and chewier than normal breast muscles. These results suggest that cooked WBC meat would likely be tougher than cooked normal meat. C1 [Chatterjee, D.; Zhuang, H.; Bowker, B. C.; Sanchez-Brambila, G.] ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. Univ Georgia, Dept Poultry Sci, Poultry Sci Bldg, Athens, GA 30602 USA. RP Zhuang, H (reprint author), ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hong.zhuang@ars.usda.gov NR 25 TC 0 Z9 0 U1 7 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD OCT PY 2016 VL 95 IS 10 BP 2449 EP 2454 DI 10.3382/ps/pew204 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA DZ9OF UT WOS:000386205600029 PM 27418659 ER PT J AU Brambila, GS Bowker, BC Zhuang, H AF Brambila, G. Sanchez Bowker, B. C. Zhuang, H. TI Comparison of sensory texture attributes of broiler breast fillets with different degrees of white striping SO POULTRY SCIENCE LA English DT Article DE broiler breast fillet; white striping; sensory; texture ID MEAT QUALITY; EATING QUALITY; SHEAR FORCE; TENDERNESS; TRAITS; GROWTH; MUSCLE; AGE AB The white striping (WS) condition in broiler meat results in increased intramuscular fat, connective tissue, and moisture loss during cooking and negatively affects product appearance and consumer acceptance of skinless chicken meat. The effect of WS on the human perception of cooked meat texture is unknown. The objective of this study was to evaluate the effects of WS on sensory texture attributes of cooked chicken breast fillets (Pectoralis major). Over three separate trial days, a total of 105 breast butterfly fillets were collected from the deboning line of a commercial broiler processing plant. Fillets were classified according to the degree of WS (normal, moderate, severe) and stored at -20A degrees C until use. Fourteen representative fillets from each category were cooked directly from the frozen state to an endpoint temperature of 78A degrees C and evaluated by a 7-member trained panel for five texture attributes: cohesiveness, hardness, juiciness, rate of breakdown, and chewiness. There were no differences (P > 0.05) for juiciness or the rate of breakdown between the fillets based on the degree of WS. Among the three WS groups, however, differences (P < 0.05) in cohesiveness, hardness, and chewiness were observed. For these attributes, the mean intensity scores of fillets with severe WS were consistently highest among the groups. There were no differences (P > 0.05) between the normal and moderate WS fillets. These data suggest that the severe WS condition was perceived to be harder, more cohesive, and chewier than either normal or moderate WS fillets by panelists. C1 [Brambila, G. Sanchez; Bowker, B. C.; Zhuang, H.] ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Brambila, GS (reprint author), ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM gabriela.sbrambila@ars.usda.gov NR 26 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD OCT PY 2016 VL 95 IS 10 BP 2472 EP 2476 DI 10.3382/ps/pew165 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA DZ9OF UT WOS:000386205600032 ER PT J AU Li, M Jordan, NR Koide, RT Yannarell, AC Davis, AS AF Li, Meng Jordan, Nicholas R. Koide, Roger T. Yannarell, Anthony C. Davis, Adam S. TI Meta-Analysis of Crop and Weed Growth Responses to Arbuscular Mycorrhizal Fungi: Implications for Integrated Weed Management SO WEED SCIENCE LA English DT Article DE Integrated weed management; mycorrhizal growth response; mycorrhizal host status ID PLANT COMMUNITY STRUCTURE; DIFFERENTIAL RESPONSE; DROUGHT STRESS; COVER CROPS; SOIL; DIVERSITY; IMPACT; PHOSPHORUS; SYMBIOSIS; NITROGEN AB Integrated weed management (IWM) relies upon multiple chemical, physical, or biological weed management techniques to achieve an acceptable level of weed control. Agents that selectively suppress weeds but not crops and that can be manipulated in agriculture will be promising components for inclusion in IWM. We used a meta-analytic approach to investigate the potential of arbuscular mycorrhizal fungi (AMF) to contribute to IWM. We quantified the effect of crop and weed host status (strong and weak AMF hosts are divided in this study by a 10% root length colonization threshold), AMF diversity (single vs. mixed), and soil N and P fertility management on plant mycorrhizal growth responses (MGRs). Our results indicated that weak host weeds had consistently lower MGRs than strong host crops in both controlled and field conditions. Moreover, these differences in MGRs between weak host weeds and strong host crops were more pronounced under mixed AMF inoculum and low N and P nutrient availability. In contrast, MGR of strong host weeds was not different from strong host crops in general. However, we observed a wide range of MGRs among strong host weeds, some of which had much lower MGRs than strong host crops. In addition, in the presence of N and P fertilizers, strong host crops had a stronger positive response to AMF than strong host weeds. Thus, our meta-analysis indicates that AMF have potential to contribute to weed control by direct and indirect pathways: directly suppress weak host weeds, and indirectly suppress some strong host weeds mediating by competitive effects exerted by strong host crops. We suggest that management practices affecting AMF diversity and crop and weed mycorrhizal responses could be chosen to improve the contribution of AMF to IWM. Better understanding is needed of crop-weed-AMF interactions and management practices that enhance this form of weed management. C1 [Li, Meng] Univ Illinois Champaign Urbana, Dept Crop Sci, Urbana, IL 61801 USA. [Jordan, Nicholas R.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Koide, Roger T.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Yannarell, Anthony C.] Univ Illinois Champaign Urbana, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Davis, Adam S.] ARS, USDA, Urbana, IL 61801 USA. RP Li, M (reprint author), Univ Illinois Champaign Urbana, Dept Crop Sci, Urbana, IL 61801 USA. EM mengli5@illinois.edu FU USDA-ARS; USDA-NIFA [2011-6703-30343] FX We thank Drs. J. Dalling and D. Eastburn for the time they took to review preliminary drafts of this manuscript, and two anonymous reviewers for their constructive comments. This work was supported by USDA-ARS and USDA-NIFA Project #2011-6703-30343. NR 74 TC 0 Z9 0 U1 18 U2 18 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD OCT-DEC PY 2016 VL 64 IS 4 BP 642 EP 652 DI 10.1614/WS-D-16-00050.1 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DZ7OH UT WOS:000386055100011 ER PT J AU Grey, TL Rucker, K Webster, TM Luo, XL AF Grey, Timothy L. Rucker, Keith Webster, Theodore M. Luo, Xuelin TI High-Density Plantings of Olive Trees Are Tolerant to Repeated Applications of Indaziflam SO WEED SCIENCE LA English DT Article DE Soil residual herbicide; crop injury ID SOIL; PREEMERGENCE; HERBICIDES AB Olive production in the southeastern United States has recently begun to increase from demand for locally produced virgin olive oil. With no established commercial production as a reference, information about the effects of indaziflam residual herbicide on newly established trees was evaluated over time for up to 3 yr on loamy sand soils. Multiple spring and autumn applications of indaziflam at different rates were applied to the same newly planted or 1-yr-old olive trees in different experiments in consecutive years. Visual injury, height, and caliper diameter measures were taken monthly during the growing season up to six times. Regression analysis of treatments over time indicated no differences in olive tree growth for plots treated with indaziflam at 38, 75, or 150 g ai ha(-1) up to five times in 3 yr, compared with nontreated controls. This information will be beneficial as olive growers seek viable weed control options when establishing new groves in the region. C1 [Grey, Timothy L.; Luo, Xuelin] Univ Georgia, Tifton Campus,2360 Rainwater Rd, Tifton, GA 31793 USA. [Rucker, Keith] Bayer Crop Sci, Tifton, GA 31794 USA. [Webster, Theodore M.] USDA ARS, Davis Rd, Tifton, GA 31793 USA. RP Grey, TL (reprint author), Univ Georgia, Tifton Campus,2360 Rainwater Rd, Tifton, GA 31793 USA. EM tgrey@uga.edu FU University of Georgia College of Agriculture; Bayer Crop Science FX The authors thank Kevin Shaw, Jason Shaw, and Sam Shaw of Georgia Olive Farms in Lakeland, GA, for providing materials for this research trial. This research was partially funded by the University of Georgia College of Agriculture and Bayer Crop Science. Technical support was provided by Sidney Cromer, Brian Blanchett, Fritz Turpin II, and Haydon Davis, with additional statistical support by Jerry Davis, Experimental Statistics Department, University of Georgia, Griffin Campus. NR 30 TC 0 Z9 0 U1 3 U2 3 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD OCT-DEC PY 2016 VL 64 IS 4 BP 766 EP 771 DI 10.1614/WS-D-16-00025.1 PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DZ7OH UT WOS:000386055100023 ER PT J AU Blackwell, BF DeVault, TL Fernandez-Juricic, E Gese, EM Gilbert-Norton, L Breck, SW AF Blackwell, Bradley F. DeVault, Travis L. Fernandez-Juricic, Esteban Gese, Eric M. Gilbert-Norton, Lynne Breck, Stewart W. TI No single solution: application of behavioural principles in mitigating human-wildlife conflict SO ANIMAL BEHAVIOUR LA English DT Article DE animal-vehicle collisions; anthropogenic disturbance; antipredator behaviour; carnivore-livestock depredation; human-wildlife conflict; perceived risk; sensory ecology ID ROAD MORTALITY; WOLF DEPREDATION; DIFFERENTIAL RESPONSES; CARNIVORE ATTACKS; COYOTE PREDATION; DOMESTIC SHEEP; UNITED-STATES; VULPES-VULPES; VISUAL-ACUITY; BLACK BEARS AB There is no proverbial silver bullet for mitigating human-wildlife conflict, but the study of animal behaviour is foundational to solving issues of coexistence between people and wild animals. Our purpose is to examine the theoretical and applied role that behavioural principles play in understanding and mitigating human-wildlife conflict, and delineate gaps in behavioural theory relative to mitigating these conflicts. Specifically, we consider two different, yet contemporary, examples of human-wildlife conflict: animal-vehicle collisions and carnivore depredation of livestock. Although ostensibly unrelated, both conflict areas share common themes relative to animal behavioural responses to disturbance and perception of risk. We first place the effects on wildlife due to these conflicts in the scope of population sustainability, and then examine current research relative to the following three questions. How is behavioural ecology relevant to these particular areas of conflict? Are advances toward understanding the mechanisms by which animals process information and make decisions being translated into management methods? How might management efforts be affected over time by individual behaviours, method integration and habituation/sensitization? Regarding animal-vehicle collisions, only in the last decade have researchers applied an antipredator theoretical framework with sensory ecology to understand aspects of marine mammal, terrestrial mammal and bird responses to vehicle approach, speed and associated stimuli. However, the size and speeds of modern vehicles demand that we improve economic models and possibly develop novel theoretical frameworks to better predict animal responses to vehicle approach. Within the context of carnivore-livestock depredation, our understanding of individual predator behaviour relative to perceived risk and factors contributing to the development of problem individuals will influence the efficacy of the most promising, nonlethal management approaches (e.g. distractive techniques, reproductive inhibition and olfactory barriers). In both cases, successful management is contingent upon a mechanistic understanding of how animals respond to disturbance and the information utilized to assess risk. Published by Elsevier Ltd on behalf of The Association for the Study of Animal Behaviour. C1 [Blackwell, Bradley F.; DeVault, Travis L.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Ohio Field Stn, Sandusky, OH 44870 USA. [Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Gese, Eric M.; Breck, Stewart W.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Gilbert-Norton, Lynne] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. RP Blackwell, BF (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Ohio Field Stn, Sandusky, OH 44870 USA. EM bradley.f.blackwell@aphis.usda.gov FU U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center; Department of Biological Sciences, Purdue University; Department of Wildland Resources, Utah State University FX We thank T. Katzner and T. W. Seamans for comments on earlier versions of this manuscript. Support for B.F.B., T.L.D., E.M.G. and S.W.B. was provided by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center. Support for E.F.-J. was provided by the Department of Biological Sciences, Purdue University. Support for L.G.-N. was provided by the Department of Wildland Resources, Utah State University. NR 171 TC 0 Z9 0 U1 52 U2 52 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0003-3472 EI 1095-8282 J9 ANIM BEHAV JI Anim. Behav. PD OCT PY 2016 VL 120 BP 245 EP 254 DI 10.1016/j.anbehav.2016.07.013 PG 10 WC Behavioral Sciences; Zoology SC Behavioral Sciences; Zoology GA DY8JL UT WOS:000385375900026 ER PT J AU Amundson, OL Larimore, EL McNeel, AK Chase, CC Cushman, RA Freetly, HC Perry, GA AF Amundson, Olivia L. Larimore, Erin L. McNeel, Anthony K. Chase, Chad C., Jr. Cushman, Robert A. Freetly, Harvey C. Perry, George A. TI Uterine environment and pregnancy rate of heifers with elevated plasma urea nitrogen SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Reproductive efficiency; Beef heifers; Fertility; Urea; Uterine pH ID DIETARY CRUDE-PROTEIN; LACTATING DAIRY-COWS; BEEF-COWS; REPRODUCTIVE-PERFORMANCE; ARTIFICIAL-INSEMINATION; DEGRADABLE PROTEIN; EMBRYO DEVELOPMENT; STANDING ESTRUS; PH; CATTLE AB Diets high in protein are associated with lower reproductive performance and changes in the uterine environment. The objective of this study was to determine the effect of elevated systemic concentrations of urea nitrogen on the uterine environment and pregnancy success in beef heifers. Heifers (n=150) were matched by breed, age, and body weight then randomly assigned to one of two dietary treatments: 1) Control (10% CP) or 2) High protein (14% CP) over three replicates (n = 40/replicate). Estrus was synchronized with an injection of PGF(2 alpha). Uterine pH, plasma urea nitrogen (PUN), ammonia, and glucose concentrations were determined on d 7 of the estrous cycle. Pregnancy status was determined by ultrasonography 30 d following the breeding season. In vitro fertilization was performed on heifers precluded from uterine analysis (n = 15/diet) to determine the effect of a High Protein diet on oocyte quality. Plasma urea concentrations were greater in the High Protein diet compared to Control (P<0.001). There was no effect of diet on plasma ammonia (P = 0.12), plasma glucose (P=0.40), uterine pH (P=0.67), interval to estrus (P=0.54), duration of estrus (P=0.38), or pregnancy rate (P=0.83). There was no effect of diet (P>0.40) on the number of oocytes collected, number of oocytes cleaved, amount of blastocysts, percentage of oocytes cleaved and percentage of blastocysts present. In summary, high nitrogen diets increased PUN concentrations in heifers; however, there were no deleterious effects on reproduction. (C) 2016 Elsevier B.V. All rights reserved. C1 [Amundson, Olivia L.; Larimore, Erin L.; Perry, George A.] South Dakota State Univ, Dept Anim Sci, Box 2170,ASC 214, Brookings, SD 57007 USA. [McNeel, Anthony K.; Chase, Chad C., Jr.; Cushman, Robert A.; Freetly, Harvey C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Perry, GA (reprint author), South Dakota State Univ, Dept Anim Sci, Box 2170,ASC 214, Brookings, SD 57007 USA. EM George.Perry@sdstate.edu OI Perry, George/0000-0002-0102-9046 FU South Dakota Agriculture Experiment Station Hatch funds; ARS Project Plan entitled "Strategies to Improve Heifer Selection and Heifer Development" [3040-31000-093-00D] FX Mention of a proprietary product does not constitute a guarantee or warranty of the product by South Dakota AES, or the authors, and does not imply its approval to the exclusion of other products that may also be suitable. This project was funded by the South Dakota Agriculture Experiment Station Hatch funds and ARS Project Plan number 3040-31000-093-00D entitled "Strategies to Improve Heifer Selection and Heifer Development". NR 43 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD OCT PY 2016 VL 173 BP 56 EP 62 DI 10.1016/j.anireprosci.2016.08.011 PG 7 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA DY7SJ UT WOS:000385329300010 PM 27609357 ER PT J AU Filatova, LY Donovan, DM Ishnazarova, NT Foster-Frey, JA Becker, SC Pugachev, VG Balabushevich, NG Dmitrieva, NF Klyachko, NL AF Filatova, Lyubov Y. Donovan, David M. Ishnazarova, Nadiya T. Foster-Frey, Juli A. Becker, Stephen C. Pugachev, Vladimir G. Balabushevich, Nadezda G. Dmitrieva, Natalia F. Klyachko, Natalia L. TI A Chimeric LysK-Lysostaphin Fusion Enzyme Lysing Staphylococcus aureus Cells: a Study of Both Kinetics of Inactivation and Specifics of Interaction with Anionic Polymers SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Fusion enzyme; Staphylolytic activity; Enzyme kinetics; Applied biocatalysis ID ANTI-INFECTIVES; ANTIMICROBIALS; ENDOLYSINS AB A staphylolytic fusion protein (chimeric enzyme K-L) was created, harboring three unique lytic activities composed of the LysK CHAP endopeptidase, and amidase domains, and the lysostaphin glycyl-glycine endopeptidase domain. To assess the potential of possible therapeutic applications, the kinetic behavior of chimeric enzyme K-L was investigated. As a protein antimicrobial, with potential antigenic properties, the biophysical effect of including chimeric enzyme K-L in anionic polymer matrices that might help reduce the immunogenicity of the enzyme was tested. Chimeric enzyme K-L reveals a high lytic activity under the following optimal ((opt)) conditions: pH(opt) 6.0-10.0, t(opt) 20-30 A degrees C, NaClopt 400-800 mM. At the working temperature of 37 A degrees C, chimeric enzyme K-L is inactivated by a monomolecular mechanism and possesses a high half-inactivation time of 12.7 A +/- 3.0 h. At storage temperatures of 22 and 4 A degrees C, a complex mechanism (combination of monomolecular and bimolecular mechanisms) is involved in the chimeric enzyme K-L inactivation. The optimal storage conditions under which the enzyme retains 100 % activity after 140 days of incubation (4 A degrees C, the enzyme concentration of 0.8 mg/mL, pH 6.0 or 7.5) were established. Chimeric enzyme K-L is included in complexes with block-copolymers of poly-l-glutamic acid and polyethylene glycol, while the enzyme activity and stability are retained, thus suggesting methods to improve the application of this fusion as an effective antimicrobial agent. C1 [Filatova, Lyubov Y.; Ishnazarova, Nadiya T.; Balabushevich, Nadezda G.; Klyachko, Natalia L.] Moscow MV Lomonosov State Univ, Dept Chem Enzymol, Fac Chem, Moscow, Russia. [Donovan, David M.; Foster-Frey, Juli A.; Becker, Stephen C.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, NEA,USDA, Beltsville, MD USA. [Pugachev, Vladimir G.] State Res Ctr Virol & Bioengn Vector, Fed Budget Inst Sci, Novosibirsk, Russia. [Dmitrieva, Natalia F.] IM Sechenov First Moscow State Med Univ, Moscow, Russia. [Klyachko, Natalia L.] Univ North Carolina Chapel Hill, UNC Eshelman Sch Pharm, Div Mol Pharmaceut, Ctr Nanotechnol Drug Delivery, Chapel Hill, NC USA. RP Filatova, LY (reprint author), Moscow MV Lomonosov State Univ, Dept Chem Enzymol, Fac Chem, Moscow, Russia. EM luboff.filatova@gmail.com FU BARD [IS-4573-12R/58-1265-2-132] FX The study was carried out by SkolTech N 182-MRA and was supported by BARD grant no. IS-4573-12R/58-1265-2-132. NR 29 TC 0 Z9 0 U1 2 U2 2 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD OCT PY 2016 VL 180 IS 3 BP 544 EP 557 DI 10.1007/s12010-016-2115-7 PG 14 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DY5JM UT WOS:000385135900011 PM 27168405 ER PT J AU Freitas, C Meerow, AW Pintaud, JC Henderson, A Noblick, L Costa, FRC Barbosa, CE Barrington, D AF Freitas, Cintia Meerow, Alan W. Pintaud, Jean-Christophe Henderson, Andrew Noblick, Larry Costa, Flavia R. C. Barbosa, Carlos E. Barrington, David TI Phylogenetic analysis of Attalea (Arecaceae): insights into the historical biogeography of a recently diversified Neotropical plant group SO BOTANICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Article; Proceedings Paper CT World Palms Sympsoium CY 2015 CL Quindio, COLOMBIA DE Bayesian inference; molecular phylogeny; neotropics; palm phylogeny; South America ID PALMS ARECACEAE; CLIMATE-CHANGE; ANDEAN UPLIFT; SOUTH-AMERICA; DRAKE PASSAGE; PATTERNS; EVOLUTION; BIODIVERSITY; FAMILY; RECOMBINATION AB We present a dated phylogenetic tree for the Neotropical genus Attalea (Arecaceae). We used six orthologues from the nuclear WRKY gene family across 98 accessions to address relationships among species and biogeographical hypotheses. We found that the formerly recognized groups within Attalea are not monophyletic and therefore there is no support for multiple genera as previously thought. Species of Attalea-like palms from the Atlantic forest form a well-supported clade sister to the Attalea species from Amazonia, the Andean valleys and Mesoamerica. Dates for the main divergence events suggest a relationship with the development of the dry forests that now span eastern South America and the now-lost Pebas Lake region in the western Amazon. Attalea crassispatha possibly colonized Hispaniola by a long-distance dispersal event, not via the land bridge Great Antilles Avian Ridge (GAAR), before the Panama channel closed. The common ancestor of Attalea appears to have been an Atlantic forest clade c. 30 Mya. The early split between the Atlantic-forest clade and the two Amazonian-northern Andean clades was probably the result of climatic changes that caused an increase in aridity in South America. (C) 2016 The Linnean Society of London. C1 [Freitas, Cintia; Henderson, Andrew] New York Bot Garden, 2900 Southern Blvd, Bronx, NY 10458 USA. [Freitas, Cintia; Barrington, David] Univ Vermont, Dept Plant Biol, 63 Carrigan Dr, Burlington, VT 05405 USA. [Meerow, Alan W.] ARS, USDA, Natl Germplasm Repository, 13601 Old Cutler Rd, Miami, FL 33158 USA. [Pintaud, Jean-Christophe] IRD, UMR 232, DIADE, DYNADIV, BP 64501,911 Ave Agropolis, F-34394 Montpellier, France. [Noblick, Larry] Montgomery Bot Ctr, 11901 Old Cutler Rd, Coral Gables, FL 33156 USA. [Costa, Flavia R. C.; Barbosa, Carlos E.] Inst Pesquisas Amazonia, Ave Ephigenio Sales 2239, BR-69011970 Manaus, Amazonas, Brazil. RP Freitas, C (reprint author), New York Bot Garden, 2900 Southern Blvd, Bronx, NY 10458 USA.; Freitas, C (reprint author), Univ Vermont, Dept Plant Biol, 63 Carrigan Dr, Burlington, VT 05405 USA. EM cintia.freitas@ufg.edu.br OI Freitas, Cintia/0000-0003-3094-8364 NR 81 TC 1 Z9 1 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4074 EI 1095-8339 J9 BOT J LINN SOC JI Bot. J. Linnean Soc. PD OCT PY 2016 VL 182 IS 2 SI SI BP 287 EP 302 DI 10.1111/boj.12466 PG 16 WC Plant Sciences SC Plant Sciences GA DZ3LL UT WOS:000385748500008 ER PT J AU Xue, XY Lan, YB Sun, Z Chang, C Hoffmann, WC AF Xue, Xinyu Lan, Yubin Sun, Zhu Chang, Chun Hoffmann, W. Clint TI Develop an unmanned aerial vehicle based automatic aerial spraying system SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE UAV; Aerial spraying; GPS navigation; Spray uniformity AB To perform plant-protection operations, an unmanned aerial vehicle (UAV) based automatic control spraying system was designed in China. The system used a highly integrated and ultra-low power MSP430 single-chip micro-computer with an independent functional module. This allowed route planning software to direct the UAV to the desired spray area. The test results of route precision showed that in a 3-4 m/s crosswind, route deviations were around 0.2 m. The result of multiple-spraying swath uniformity tests showed a minimum coefficient of variation of 25% when flying at a height of 5 m with a spraying swath of 7 m and a wind speed of 0-2 m/s. When the spraying swath was 9 m or 5 m, the coefficients were 34% and 41%, respectively. Spray uniformity for these UAV tests were superior to the Standard Requirement for ultra-low volume spraying variation coefficient, 60%. (C) 2016 Elsevier B.V. All rights reserved. C1 [Xue, Xinyu; Sun, Zhu; Chang, Chun] Nanjing Res Inst Agr Mechanizat, Minist Agr, Nanjing 210014, Jiangsu, Peoples R China. [Lan, Yubin] South China Agr Univ, Coll Engn, Guangzhou, Guangdong, Peoples R China. [Hoffmann, W. Clint] ARS, USDA, SPARC, AATRU, College Stn, TX 77845 USA. RP Lan, YB (reprint author), South China Agr Univ, Coll Engn, Guangzhou, Guangdong, Peoples R China. EM ylan@scau.edu.cn FU National High Technology Research and Development Program [2013AA10230303]; Special Fund for Agro-scientific Research in the Public Interest [201203025] FX The authors gratefully acknowledge the financial support provided by:; (1) National High Technology Research and Development Program (2013AA10230303).; (2) Special Fund for Agro-scientific Research in the Public Interest (201203025). NR 15 TC 0 Z9 0 U1 26 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD OCT PY 2016 VL 128 BP 58 EP 66 DI 10.1016/j.compag.2016.07.022 PG 9 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DY9RR UT WOS:000385473300008 ER PT J AU Ferguson, JC Chechetto, RG O'Donnell, CC Fritz, BK Hoffmann, WC Coleman, CE Chauhan, BS Adkins, SW Kruger, GR Hewitt, AJ AF Ferguson, J. Connor Chechetto, Rodolfo G. O'Donnell, Chris C. Fritz, Brad K. Hoffmann, W. Clint Coleman, Chet E. Chauhan, Bhagirath S. Adkins, Steve W. Kruger, Greg R. Hewitt, Andrew J. TI Assessing a novel smartphone application - SnapCard, compared to five imaging systems to quantify droplet deposition on artificial collectors SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Image analysis; Kromekote; Water-sensitive paper; SnapCard; Smartphone App; Sprays; Droplet size; Spray quality ID WATER-SENSITIVE PAPERS; SPRAY DROPLETS; INSECTICIDES; SIZE; IMAGES; VOLUME; FILM AB Previous work sought to compare the results from imaging software for characterising droplet coverage, but none exists examining these five software programs: Droplet Scan (R), Swath Kit (R), Deposit Scan, Image J, and Drop Vision (R)-Ag. Additionally, a freely available smartphone application (App), SnapCard was developed to provide an extension tool for in-field analysis of spray collectors, but nothing has been published regarding its comparison to other imaging software systems. The present study was conducted to compare five existing imaging software types against the new App, SnapCard. Six nozzles producing different spray qualities were selected to spray a water + Brilliant Blue Dye solution over two artificial collector types (water sensitive paper and Kromekote (R)). Each collector was assessed for percent coverage using the five imaging systems and SnapCard. Objectives of this study were: 1. To establish a baseline dataset using the sprayed cards and five commonly used imaging systems, and compare the coverage results from each. 2. Use the baseline data from Objective 1 as a measurement of precision to judge the results from SnapCard. 3. Make an assessment of SnapCard against the other imaging software type data in the study. Results showed that SnapCard has similar measured coverage means compared to other image analysis systems. For both collector types, SnapCard measured coverage within one standard deviation of the means across nozzle types. SnapCard is able to provide an immediate answer without expensive software or needing a laboratory to measure sprayed collector coverage with precise results, which further underscores its value. The other software types were not all similar for coverage, but the data followed the same trends for droplet size. Increasing the droplet size consistently decreased the coverage, across both collector types. Droplet Scan reported the highest coverage while Drop Vision-Ag and Swath Kit gave lower coverage values on water sensitive paper and Kromekote (R) collectors, respectively. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ferguson, J. Connor; Chechetto, Rodolfo G.; O'Donnell, Chris C.; Adkins, Steve W.; Hewitt, Andrew J.] Univ Queensland, Gatton, Qld 4343, Australia. [Ferguson, J. Connor] Northwest Missouri State Univ, Maryville, MO 64468 USA. [Chechetto, Rodolfo G.] Sao Paulo State Univ FCA, Dept Rural Engn, BR-18610 Sao Paulo, Brazil. [Fritz, Brad K.; Hoffmann, W. Clint; Coleman, Chet E.] ARS, USDA, 2771 F&B Rd, College Stn, TX 77845 USA. [Chauhan, Bhagirath S.] Univ Queensland, Queensland Alliance Agr & Food Innovat, Toowoomba, Qld 4350, Australia. [Kruger, Greg R.; Hewitt, Andrew J.] Univ Nebraska Lincoln, North Platte, NE 69101 USA. RP Ferguson, JC (reprint author), Northwest Missouri State Univ, Maryville, MO 64468 USA. EM cferguson@nwmissouri.edu FU Grains Research and Development Corporation of Australia (GRDC) FX The authors acknowledge the Grains Research and Development Corporation of Australia (GRDC) for their support of this work through the project titled "Options for improved, insecticide and fungicide use and canopy penetration in cereals and canola." The authors also would like to thank John Moore of the Department of Agriculture and Food WA for his support of travel in this study. NR 31 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD OCT PY 2016 VL 128 BP 193 EP 198 DI 10.1016/j.compag.2016.08.022 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DY9RR UT WOS:000385473300021 ER PT J AU Fletcher, RS Reddy, KN AF Fletcher, Reginald S. Reddy, Krishna N. TI Random forest and leaf multispectral reflectance data to differentiate three soybean varieties from two pigweeds SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Light reflectance; Machine learning; Palmer amaranth; Redroot pigweed ID LAND-COVER CLASSIFICATION; GLYCINE-MAX; HYPERSPECTRAL REFLECTANCE; VARIABLE IMPORTANCE; PALMER AMARANTH; INTERFERENCE; CLASSIFIERS; CROP; DISCRIMINATION; ACCURACY AB Accurate weed identification is a prerequisite for implementing site-specific weed management in crop production. Palmer amaranth (Amaranthus palmeri S. Wats.) and redroot pigweed (Amaranthus retroflexus L.) are two common pigweeds that reduce soybean [Glycine max (L.) Men.] yields in the southeastern United States. The objective of this study was to evaluate leaf multispectral reflectance data as input into the random forest machine learning algorithm to differentiate three soybean varieties (Progeny 4928, Progeny 5160, and Progeny 5460) from Palmer amaranth and redroot pigweed. Leaf reflectance measurements of soybean, Palmer amaranth, and redroot pigweed plants grown in a greenhouse were collected with a plant contact probe attached to a hyperspectral spectroradiometer. Data were obtained at the vegetative growth stage of the plants on two dates, June 30, 2014, and September 17, 2014. The hyperspectral data were aggregated to sixteen multispectral bands (viz. coastal, blue, green, yellow, red, red-edge, near-infrared 1 and 2, and shortwave-infrared 1-8) mimicking those recorded by the WorldView-3 satellite sensor. Classifications were binary, meaning one soybean variety versus one weed tested per classification. Random forest classification accuracies were determined with a confusion matrix, incorporating user's, producer's, and overall accuracies and the kappa coefficient. User's, producer's, and overall accuracies of the soybean weed classifications ranged from 93.8% to 100%. Kappa results (values of 0.93-0.97) indicated an excellent agreement between the classes predicted by the models and the actual reference data. Shortwave-infrared bands were ranked the most important variables for distinguishing the pigweeds from the soybean varieties. These results suggest that random forest and leaf multispectral reflectance data could be used as tools to differentiate soybean from two pig weeds with a potential application of this technology in site-specific weed management programs. Published by Elsevier B.V. C1 [Fletcher, Reginald S.; Reddy, Krishna N.] ARS, USDA, Crop Prod Syst Res Unit, POB 350, Stoneville, MS 38776 USA. RP Fletcher, RS (reprint author), ARS, USDA, Crop Prod Syst Res Unit, POB 350, Stoneville, MS 38776 USA. EM reginald.fletcher@ars.usda.gov NR 46 TC 1 Z9 1 U1 12 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD OCT PY 2016 VL 128 BP 199 EP 206 DI 10.1016/j.compag.2016.09.004 PG 8 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DY9RR UT WOS:000385473300022 ER PT J AU Iannone, BV Potter, KM Guo, QF Liebhold, AM Pijanowski, BC Oswalt, CM Fei, SL AF Iannone, Basil V., III Potter, Kevin M. Guo, Qinfeng Liebhold, Andrew M. Pijanowski, Bryan C. Oswalt, Christopher M. Fei, Songlin TI Biological invasion hotspots: a trait-based perspective reveals new sub-continental patterns SO ECOGRAPHY LA English DT Article ID FOREST PLANT INVASIONS; FUNCTIONAL DIVERSITY; ECOSYSTEM PROCESSES; COMMUNITY ECOLOGY; SPECIES RICHNESS; NEW-ENGLAND; IMPACTS; CLIMATE; INTRODUCTIONS; ASSOCIATION AB Invader traits (including plant growth form) may play an important, and perhaps overlooked, role in determining macroscale patterns of biological invasions and therefore warrant greater consideration in future investigations aimed at understanding these patterns. To assess this need, we used empirical data from a national-level survey of forest in the contiguous 48 states of the USA to identify geographic hotspots of forest plant invasion for three distinct invasion characteristics: invasive species richness, trait richness (defined as the number of the five following plant growth forms represented by the invasive plants present at a given location: forbs, grasses, shrubs, trees, and vines), and species richness within each growth form. Three key findings emerged. 1) The hotspots identified encompassed from 9 to 23% of the total area of our study region, thereby revealing many forests to be not only invaded, but highly invaded. 2) Substantial spatial disagreement among hotspots of invasive species richness, invasive trait richness, and species richness of invasive plants within each growth form revealed many locations to be hotspots for invader traits, or for particular growth forms of invasive plants, rather than for invasive plants in general. 3) Despite eastern forests exhibiting higher levels of plant invasion than western forests, species richness for invasive forbs and grasses in the west were respectively greater than and equivalent to levels found in the east. Contrasting patterns between eastern and western forests in the number of invasive species detected for each growth form combined with the spatial disagreement found among hotspot types suggests trait-based variability in invasion drivers. Our findings reveal invader traits to be an important contributor to macroscale invasion patterns. C1 [Iannone, Basil V., III; Pijanowski, Bryan C.; Fei, Songlin] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Potter, Kevin M.] North Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC USA. [Guo, Qinfeng] US Forest Serv, USDA, Southern Res Stn, Asheville, NC USA. [Liebhold, Andrew M.] US Forest Serv, USDA, Northern Res Stn, Morgantown, WV USA. [Oswalt, Christopher M.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN USA. RP Fei, SL (reprint author), Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. EM sfei@purdue.edu OI Potter, Kevin/0000-0002-7330-5345; Fei, Songlin/0000-0003-2772-0166 FU NSF [1241932]; USDA Forest Service [14-CS-11330110-042]; North Carolina State Univ. [14-CS-11330110-042] FX This research was supported by NSF MacroSystems Biology grant # 1241932 and through a Cost Share Agreement 14-CS-11330110-042 between the USDA Forest Service and North Carolina State Univ. Thanks to all who collected the FIA data used here, as well as to Richard A. McCullough, Elizabeth LaPoint, Beth Schultz, Andrew Gray, and Chris Witt from USFS who assisted with data compilation and acquisition, to Jarrod Doucette and Johanna Desprez who provided advice regarding GIS procedures. NR 63 TC 0 Z9 0 U1 22 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-7590 EI 1600-0587 J9 ECOGRAPHY JI Ecography PD OCT PY 2016 VL 39 IS 10 BP 961 EP 969 DI 10.1111/ecog.01973 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DZ0XR UT WOS:000385563500006 ER PT J AU Hood, SM Baker, S Sala, A AF Hood, Sharon M. Baker, Stephen Sala, Anna TI Fortifying the forest: thinning and burning increase resistance to a bark beetle outbreak and promote forest resilience SO ECOLOGICAL APPLICATIONS LA English DT Article DE delayed mortality; Dendroctonus ponderosae; fire-adaptations; fuel treatment; Montana, USA; oleoresin; Pinus ponderosa; plant defense; silviculture; succession ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; FUEL-REDUCTION TREATMENTS; PINE/DOUGLAS-FIR FORESTS; SUPPORT CURRENT POLICY; COLORADO FRONT RANGE; PONDEROSA PINE; RESIN DEFENSES; CLIMATE-CHANGE; RESTORATION TREATMENTS AB Fire frequency in low-elevation coniferous forests in western North America has greatly declined since the late 1800s. In many areas, this has increased tree density and the proportion of shade-tolerant species, reduced resource availability, and increased forest susceptibility to forest insect pests and high-severity wildfire. In response, treatments are often implemented with the goal of increasing ecosystem resilience by increasing resistance to disturbance. We capitalized on an existing replicated study of fire and stand density treatments in a ponderosa pine (Pinus ponderosa)-Douglas-fir (Pseudotsuga menziesii) forest in western Montana, USA, that experienced a naturally occurring mountain pine beetle (MPB; Dendroctonus ponderosae) outbreak 5 yr after implementation of fuels treatments. We explored whether treatment effects on tree-level defense and stand structure affected resistance to MPB. Mortality from MPB was highest in the denser, untreated control and burn-only treatments, with approximately 50% and 39%, respectively, of ponderosa pine killed during the outbreak, compared to almost no mortality in the thin-only and thin-burn treatments. Thinning treatments, with or without fire, dramatically increased tree growth and resin ducts relative to control and burn-only treatments. Prescribed burning did not increase resin ducts but did cause changes in resin chemistry that may have affected MPB communication and lowered attack success. While ponderosa pine remained dominant in the thin and thin-burn treatments after the outbreak, the high pine mortality in the control and burn-only treatment caused a shift in species dominance to Douglas-fir. The high Douglas-fir component in the control and burn-only treatments due to 20th century fire exclusion, coupled with high pine mortality from MPB, has likely reduced resilience of this forest beyond the ability to return to a ponderosa pine-dominated system in the absence of further fire or mechanical treatment. Our results show treatments designed to increase resistance to high-severity fire in ponderosa pine-dominated forests in the Northern Rockies can also increase resistance to MPB, even during an outbreak. This study suggests that fuel and restoration treatments in fire-dependent ponderosa pine forests that reduce tree density increase ecosystem resilience in the short term, while the reintroduction of fire is important for long-term resilience. C1 [Hood, Sharon M.; Baker, Stephen] US Forest Serv, Fire Fuel & Smoke Sci Program, Rocky Mt Res Stn, USDA, 5775 Highway 10 W, Missoula, MT 59808 USA. [Hood, Sharon M.; Sala, Anna] Univ Montana, Div Biol Sci, 32 Campus Dr, Missoula, MT 59812 USA. [Hood, Sharon M.] 5775 Highway 10 W, Missoula, MT 59808 USA. RP Hood, SM (reprint author), US Forest Serv, Fire Fuel & Smoke Sci Program, Rocky Mt Res Stn, USDA, 5775 Highway 10 W, Missoula, MT 59808 USA.; Hood, SM (reprint author), Univ Montana, Div Biol Sci, 32 Campus Dr, Missoula, MT 59812 USA.; Hood, SM (reprint author), 5775 Highway 10 W, Missoula, MT 59808 USA. EM sharonmhood@fs.fed.us OI Hood, Sharon/0000-0002-9544-8208 FU USDA Forest Service, Rocky Mountain Research Station, Fire, Fuel, and Smoke Science Program; IM-SURE Program of National Science Foundation [0755560, 1157101]; National Science Foundation EPSCoR Track-1 [EPS-1101342, EPS-IIA-1443108]; National Fire and Fire Surrogate Project [FFS 99-S-01]; U.S. Joint Fire Science Program FX Funding for this work was provided by the USDA Forest Service, Rocky Mountain Research Station, Fire, Fuel, and Smoke Science Program, the IM-SURE Program of National Science Foundation Award 0755560 and 1157101, and National Science Foundation EPSCoR Track-1 EPS-1101342 and EPS-IIA-1443108 (INSTEP 3). This is Contribution Number 223 of the National Fire and Fire Surrogate Project (FFS 99-S-01), funded by the U.S. Joint Fire Science Program. We thank Alexandra Ginter, Megan Richardson, Alexaida Echevarria, and numerous additional field crews for their hard work. Celia Boone provided critical advice on monoterpene extraction techniques. The comments of John Maron, Cara Nelson, Barbara Bentz, Ragan Callaway, Carolyn Sieg, and three anonymous reviewers improved the final manuscript. NR 123 TC 1 Z9 1 U1 36 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD OCT PY 2016 VL 26 IS 7 BP 1984 EP 2000 DI 10.1002/eap.1363 PG 17 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DZ1TR UT WOS:000385623900003 PM 27755724 ER PT J AU Kline, JD Harmon, ME Spies, TA Morzillo, AT Pabst, RJ McComb, BC Schnekenburger, F Olsen, KA Csuti, B Vogeler, JC AF Kline, Jeffrey D. Harmon, Mark E. Spies, Thomas A. Morzillo, Anita T. Pabst, Robert J. McComb, Brenda C. Schnekenburger, Frank Olsen, Keith A. Csuti, Blair Vogeler, Jody C. TI Evaluating carbon storage, timber harvest, and habitat possibilities for a Western Cascades (USA) forest landscape SO ECOLOGICAL APPLICATIONS LA English DT Article DE ecosystem services; forest management; landscape analysis; trade-offs ID MULTI-OWNERSHIP PROVINCE; OREGON COAST RANGE; PACIFIC-NORTHWEST; SILVICULTURAL PRACTICES; WILDLIFE HABITAT; JOINT PRODUCTION; HEMLOCK FORESTS; UNITED-STATES; BIODIVERSITY; STORES AB Forest policymakers and managers have long sought ways to evaluate the capability of forest landscapes to jointly produce timber, habitat, and other ecosystem services in response to forest management. Currently, carbon is of particular interest as policies for increasing carbon storage on federal lands are being proposed. However, a challenge in joint production analysis of forest management is adequately representing ecological conditions and processes that influence joint production relationships. We used simulation models of vegetation structure, forest sector carbon, and potential wildlife habitat to characterize landscape-level joint production possibilities for carbon storage, timber harvest, and habitat for seven wildlife species across a range of forest management regimes. We sought to (1) characterize the general relationships of production possibilities for combinations of carbon storage, timber, and habitat, and (2) identify management variables that most influence joint production relationships. Our 160 000-ha study landscape featured environmental conditions typical of forests in the Western Cascade Mountains of Oregon (USA). Our results indicate that managing forests for carbon storage involves trade-offs among timber harvest and habitat for focal wildlife species, depending on the disturbance interval and utilization intensity followed. Joint production possibilities for wildlife species varied in shape, ranging from competitive to complementary to compound, reflecting niche breadth and habitat component needs of species examined. Managing Pacific Northwest forests to store forest sector carbon can be roughly complementary with habitat for Northern Spotted Owl, Olive-sided Flycatcher, and red tree vole. However, managing forests to increase carbon storage potentially can be competitive with timber production and habitat for Pacific marten, Pileated Woodpecker, and Western Bluebird, depending on the disturbance interval and harvest intensity chosen. Our analysis suggests that joint production possibilities under forest management regimes currently typical on industrial forest lands (e.g., 40- to 80-yr rotations with some tree retention for wildlife) represent but a small fraction of joint production outcomes possible in the region. Although the theoretical boundaries of the production possibilities sets we developed are probably unachievable in the current management environment, they arguably define the long-term potential of managing forests to produce multiple ecosystem services within and across multiple forest ownerships. C1 [Kline, Jeffrey D.; Spies, Thomas A.] US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Harmon, Mark E.; Pabst, Robert J.; Schnekenburger, Frank; Olsen, Keith A.] Oregon State Univ, Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Morzillo, Anita T.] Univ Connecticut, Dept Nat Resources & Environm, 1376 Storrs Rd, Storrs, CT 06269 USA. [McComb, Brenda C.] Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. [Csuti, Blair] Oregon State Univ, Inst Nat Resources, POB 751, Portland, OR 97331 USA. [Vogeler, Jody C.] US Forest Serv, Lab Applicat Remote Sensing Ecol, USDA, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Vogeler, Jody C.] Oregon State Univ, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Kline, JD (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM JKline@fs.fed.us FU National Aeronautical and Space Administration ROSES grant [10-CARBON 10-45]; National Science Foundation [LTER DEB-0823380]; USDA Forest Service, Pacific Northwest Research Station FX Funding was provided by the National Aeronautical and Space Administration ROSES grant (10-CARBON 10-45), the National Science Foundation (Andrews LTER DEB-0823380), and the USDA Forest Service, Pacific Northwest Research Station. NR 57 TC 0 Z9 0 U1 16 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD OCT PY 2016 VL 26 IS 7 BP 2044 EP 2059 DI 10.1002/eap.1358 PG 16 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DZ1TR UT WOS:000385623900007 PM 27755743 ER PT J AU Alexandre, PM Stewart, SI Keuler, NS Clayton, MK Mockrin, MH Bar-Massada, A Syphard, AD Radeloff, VC AF Alexandre, Patricia M. Stewart, Susan I. Keuler, Nicholas S. Clayton, Murray K. Mockrin, Miranda H. Bar-Massada, Avi Syphard, Alexandra D. Radeloff, Volker C. TI Factors related to building loss due to wildfires in the conterminous United States SO ECOLOGICAL APPLICATIONS LA English DT Article DE building loss; ecoregions; linear and logistic regression; national analysis; wildfires; wildland-urban interface ID WILDLAND-URBAN INTERFACE; FOREST STRUCTURE; FIRE FREQUENCY; FUEL BREAKS; WESTERN US; PATTERNS; CALIFORNIA; IGNITION; MODEL; MANAGEMENT AB Wildfire is globally an important ecological disturbance affecting biochemical cycles and vegetation composition, but also puts people and their homes at risk. Suppressing wildfires has detrimental ecological effects and can promote larger and more intense wildfires when fuels accumulate, which increases the threat to buildings in the wildland-urban interface (WUI). Yet, when wildfires occur, typically only a small proportion of the buildings within the fire perimeter are lost, and the question is what determines which buildings burn. Our goal was to examine which factors are related to building loss when a wildfire occurs throughout the United States. We were particularly interested in the relative roles of vegetation, topography, and the spatial arrangement of buildings, and how their respective roles vary among ecoregions. We analyzed all fires that occurred within the conterminous United States from 2000 to 2010 and digitized which buildings were lost and which survived according to Google Earth historical imagery. We modeled the occurrence as well as the percentage of buildings lost within clusters using logistic and linear regression. Overall, variables related to topography and the spatial arrangement of buildings were more frequently present in the best 20 regression models than vegetation-related variables. In other words, specific locations in the landscape have a higher fire risk, and certain development patterns can exacerbate that risk. Fire policies and prevention efforts focused on vegetation management are important, but insufficient to solve current wildfire problems. Furthermore, the factors associated with building loss varied considerably among ecoregions suggesting that fire policy applied uniformly across the United States will not work equally well in all regions and that efforts to adapt communities to wildfires must be regionally tailored. C1 [Alexandre, Patricia M.; Stewart, Susan I.; Radeloff, Volker C.] Univ Wisconsin, SILVIS Lab, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. [Keuler, Nicholas S.; Clayton, Murray K.] Univ Wisconsin, Dept Stat, 1300 Univ Ave, Madison, WI 53706 USA. [Mockrin, Miranda H.] US Forest Serv, Rocky Mt Res Stn, USDA, 2253 Res Pk Blvd,Suite 350, Baltimore, MD 21228 USA. [Bar-Massada, Avi] Univ Haifa, Dept Biol & Environm, Haifa, Israel. [Syphard, Alexandra D.] Conservat Biol Inst, 10423 Sierra Vista Ave, La Mesa, CA 91941 USA. RP Alexandre, PM (reprint author), Univ Wisconsin, SILVIS Lab, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM patmalico@fulbrightmail.org OI Alexandre, Patricia/0000-0003-1242-1710 FU Rocky Mountain Research Station; Northern Research Station of the USDA Forest Service; Fulbright Exchange program fellowship; Foundation for Science and Technology - POPH - QREN - Tipology 4.1 - European Social Fund [SFRH/BD/92960/2013]; MEC National Fund FX This work was supported by a research joint venture agreement with the Rocky Mountain Research Station and Northern Research Station of the USDA Forest Service, by a Fulbright Exchange program fellowship awarded to Patricia Alexandre, and by a PhD fellowship provided by the Foundation for Science and Technology to Patricia Alexandre in 2014 (FCT - Portugal - reference: SFRH/BD/92960/2013, financed by POPH - QREN - Tipology 4.1 - Advanced formation funded by the European Social Fund and by the MEC National Fund). We thank J. Jenness for his help with the Topographic Position Index tool extension for ArcGis, D. Helmers, C. Munteanu, P. Culbert, and M. Beighley for their advice and suggestions; J. Orestes and T. Henriques for their support with glmulti R package, and C. Frederick, S. Roberts, P. LaPhillip, A. Ciurro, and A. Bontje for their help with data collection. We also thank Dr. Wilcox and two anonymous reviewers who provided constructive feedback that greatly improved our manuscript. NR 71 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD OCT PY 2016 VL 26 IS 7 BP 2323 EP 2338 DI 10.1002/eap.1376 PG 16 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DZ1TR UT WOS:000385623900027 PM 27755741 ER PT J AU Davis, AJ Hooten, MB Miller, RS Farnsworth, ML Lewis, J Moxcey, M Pepin, KM AF Davis, Amy J. Hooten, Mevin B. Miller, Ryan S. Farnsworth, Matthew L. Lewis, Jesse Moxcey, Michael Pepin, Kim M. TI Inferring invasive species abundance using removal data from management actions SO ECOLOGICAL APPLICATIONS LA English DT Article DE Bayesian hierarchical model; catch-effort method; feral swine; invasive species; population monitoring; removal sampling; Sus scrofa ID RANGE EXPANSION; FERAL PIGS; CALIFORNIA; ERADICATION; POPULATIONS; WILDLIFE; MODELS; SIZES AB Evaluation of the progress of management programs for invasive species is crucial for demonstrating impacts to stakeholders and strategic planning of resource allocation. Estimates of abundance before and after management activities can serve as a useful metric of population management programs. However, many methods of estimating population size are too labor intensive and costly to implement, posing restrictive levels of burden on operational programs. Removal models are a reliable method for estimating abundance before and after management using data from the removal activities exclusively, thus requiring no work in addition to management. We developed a Bayesian hierarchical model to estimate abundance from removal data accounting for varying levels of effort, and used simulations to assess the conditions under which reliable population estimates are obtained. We applied this model to estimate site-specific abundance of an invasive species, feral swine (Sus scrofa), using removal data from aerial gunning in 59 site/time-frame combinations (480-19,600 acres) throughout Oklahoma and Texas, USA. Simulations showed that abundance estimates were generally accurate when effective removal rates (removal rate accounting for total effort) were above 0.40. However, when abundances were small (<50) the effective removal rate needed to accurately estimates abundances was considerably higher (0.70). Based on our post-validation method, 78% of our site/time frame estimates were accurate. To use this modeling framework it is important to have multiple removals (more than three) within a time frame during which demographic changes are minimized (i.e., a closed population; <= 3 months for feral swine). Our results show that the probability of accurately estimating abundance from this model improves with increased sampling effort (8+ flight hours across the 3-month window is best) and increased removal rate. Based on the inverse relationship between inaccurate abundances and inaccurate removal rates, we suggest auxiliary information that could be collected and included in the model as covariates (e.g., habitat effects, differences between pilots) to improve accuracy of removal rates and hence abundance estimates. C1 [Davis, Amy J.; Pepin, Kim M.] USDA, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. [Hooten, Mevin B.] Colorado State Univ, US Geol Survey, Colorado Cooperat Fish & Wildlife Res Unit, Ft Collins, CO 80523 USA. [Hooten, Mevin B.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Hooten, Mevin B.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Miller, Ryan S.] USDA, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave, Ft Collins, CO 80526 USA. [Farnsworth, Matthew L.; Lewis, Jesse] Conservat Sci Partners, 5 Old Town Sq,Suite 205, Ft Collins, CO 80524 USA. [Moxcey, Michael] USDA, Wildlife Serv, 2150 Ctr Ave, Ft Collins, CO 80526 USA. RP Davis, AJ (reprint author), USDA, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM amy.j.davis@aphis.usda.gov OI Miller, Ryan/0000-0003-3892-0251 FU U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services division FX Funding was provided by the U.S. Department of Agriculture Animal and Plant Health Inspection Service Wildlife Services division. Also, thanks to Mark Lutman and Michael Marlow for help with obtaining MIS data and discussions about techniques for removing feral swine. Many thanks to Dale Nolte, Mike Bodenchuk, and Kevin Grant for helpful discussions about the MIS data and control activities. We would also like to thank two anonymous reviewers and the subject matter editor for their constructive reviews. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the United States government. NR 27 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD OCT PY 2016 VL 26 IS 7 BP 2339 EP 2346 DI 10.1002/eap.1383 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DZ1TR UT WOS:000385623900028 PM 27755739 ER PT J AU Davis, AS Fu, XH Schutte, BJ Berhow, MA Dalling, JW AF Davis, Adam S. Fu, Xianhui Schutte, Brian J. Berhow, Mark A. Dalling, James W. TI Interspecific variation in persistence of buried weed seeds follows trade-offs among physiological, chemical, and physical seed defenses SO ECOLOGY AND EVOLUTION LA English DT Article DE chemical defense; dormancy; phenolic compounds; seed coat strength; seed defense syndromes; seed half-life; seed trait covariance; soil seed bank; weed management; weed seedbank ecology ID VELVETLEAF ABUTILON-THEOPHRASTI; COMMON WATERHEMP; SETARIA-FABERI; GIANT FOXTAIL; SOIL; EMERGENCE; DORMANCY; DEPTH; VIABILITY; CROP AB 1. Soil seedbanks drive infestations of annual weeds, yet weed management focuses largely on seedling mortality. As weed seedbanks increasingly become reservoirs of herbicide resistance, species-specific seedbank management approaches will be essential to weed control. However, the development of seedbank management strategies can only develop from an understanding of how seed traits affect persistence. 2. We quantified interspecific trade-offs among physiological, chemical, and physical traits of weed seeds and their persistence in the soil seedbank in a common garden study. Seeds of 11 annual weed species were buried in Savoy, IL, from 2007 through 2012. Seedling recruitment was measured weekly and seed viability measured annually. Seed physiological (dormancy), chemical (phenolic compound diversity and concentration; invertebrate toxicity), and physical traits (seed coat mass, thickness, and rupture resistance) were measured. 3. Seed half-life in the soil (t(0.5)) showed strong interspecific variation (F-10,F-30 = 15, p < .0001), ranging from 0.25 years (Bassia scoparia) to 2.22 years (Abutilon theophrasti). Modeling covariances among seed traits and seedbank persistence quantified support for two putative defense syndromes (physiological-chemical and physical-chemical) and highlighted the central role of seed dormancy in controlling seed persistence. 4. A quantitative comparison between our results and other published work indicated that weed seed dormancy and seedbank persistence are linked across diverse environments and agroecosystems. Moreover, among seedbank-forming early successional plant species, relative investment in chemical and physical seed defense varies with seedbank persistence. 5. Synthesis and applications. Strong covariance among weed seed traits and persistence in the soil seedbank indicates potential for seedbank management practices tailored to specific weed species. In particular, species with high t(0.5) values tend to invest less in chemical defenses. This makes them highly vulnerable to physical harvest weed seed control strategies, with small amounts of damage resulting in their full decay. C1 [Davis, Adam S.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Fu, Xianhui] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Schutte, Brian J.] New Mexico State Univ, Las Cruces, NM 88003 USA. [Berhow, Mark A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Dalling, James W.] Univ Illinois, Dept Plant Biol, Urbana, IL USA. RP Davis, AS (reprint author), ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM asdavis1@illinois.edu FU USDA-ARS FX Funding for this work was provided by USDA-ARS (ASD, MAB). NR 45 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD OCT PY 2016 VL 6 IS 19 BP 6836 EP 6845 DI 10.1002/ece3.2415 PG 10 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DZ1UJ UT WOS:000385626100007 ER PT J AU Thomashow, LS AF Thomashow, Linda S. TI Induced systemic resistance: a delicate balance SO ENVIRONMENTAL MICROBIOLOGY REPORTS LA English DT Article ID FLUORESCENT PSEUDOMONAS; BIOLOGICAL-CONTROL; CMR12A; BIOSYNTHESIS; LIPOPEPTIDES; BIOCONTROL; PHENAZINES; ANTIBIOTICS; RHIZOSPHERE; INDUCTION C1 [Thomashow, Linda S.] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, 365 Johnson Hall, Pullman, WA 99164 USA. RP Thomashow, LS (reprint author), Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, 365 Johnson Hall, Pullman, WA 99164 USA. EM linda.thomashow@ars.usda.gov NR 34 TC 0 Z9 0 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1758-2229 J9 ENV MICROBIOL REP JI Environ. Microbiol. Rep. PD OCT PY 2016 VL 8 IS 5 BP 560 EP 563 DI 10.1111/1758-2229.12474 PG 4 WC Environmental Sciences; Microbiology SC Environmental Sciences & Ecology; Microbiology GA DZ5AY UT WOS:000385875300001 PM 27656865 ER PT J AU McHugh, T AF McHugh, Tara TI Microwave Processing Heats Up SO FOOD TECHNOLOGY LA English DT Article C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD OCT PY 2016 VL 70 IS 10 BP 63 EP 65 PG 3 WC Food Science & Technology SC Food Science & Technology GA DZ2TH UT WOS:000385694000015 ER PT J AU Berg, EC Morgan, TA Simmons, EA Zarnoch, SJ Scudder, MG AF Berg, Erik C. Morgan, Todd A. Simmons, Eric A. Zarnoch, Stanley J. Scudder, Micah G. TI Predicting Logging Residue Volumes in the Pacific Northwest SO FOREST SCIENCE LA English DT Article DE growing-stock removals; residue ratio; timber harvest ID SPRUCE AB Pacific Northwest forest managers seek estimates of post-timber-harvest woody residue volumes and biomass that can be related to readily available site- and tree-level attributes. To better predict residue production, researchers investigated variability in residue ratios, growing-stock residue volume per mill-delivered volume, across Idaho, Montana, Oregon, and Washington. This project presented unique sample design challenges, and the authors adopted model-based sampling to calculate the growing-stock logging residue ratio for the four-state region and produced models that relate the residue ratio to individual tree- and stand-level variables meaningful to land managers. The regionwide residue ratio was 0.0269, i.e., 26.9 ft(3) of growing-stock logging residue per 1,000 ft(3) (26.9 m(3) per 1,000 m(3)) of mill-delivered volume. Residue ratios were related to tree- and site-level variables with predictive models. Residue ratios were predicted to increase with larger small-end used diameter and decline exponentially with increasing dbh. Ratios were predicted to drop when pulp logs were removed and when timber was mechanically felled. Results from this study could be used to produce or improve residue prediction tools for land managers. C1 [Berg, Erik C.] Univ Montana, Bur Business & Econ Res, Missoula, MT 59812 USA. [Morgan, Todd A.; Simmons, Eric A.; Scudder, Micah G.] Univ Montana, Missoula, MT 59812 USA. [Zarnoch, Stanley J.] US Forest Serv, USDA, Southern Res Stn, Washington, DC 20250 USA. RP Berg, EC (reprint author), Univ Montana, Bur Business & Econ Res, Missoula, MT 59812 USA. EM erik.berg@business.umt.edu; todd.morgan@business.umt.edu; eric.simmons@business.umt.edu; szarnoch@fs.fed.us; micah.scudder@business.umt.edu FU USDA Forest Service; Northwest Advanced Renewables Alliance (NARA) - Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68005-30416]; Rocky Mountain Research Station; Forest Inventory Analysis Program, Ogden, UT FX We gratefully acknowledge the financial support of the USDA Forest Service, Rocky Mountain Research Station, Forest Inventory Analysis Program, Ogden, UT, and the Northwest Advanced Renewables Alliance (NARA) supported by Agriculture and Food Research Initiative Competitive Grant 2011-68005-30416 from the USDA National Institute of Food and Agriculture. We are indebted to Drs. John Coulston, Kevin Boston, and Grant Domke for their substantive and helpful reviews of an earlier version of the article. NR 33 TC 0 Z9 0 U1 4 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD OCT PY 2016 VL 62 IS 5 BP 564 EP 573 DI 10.5849/forsci.15-176 PG 10 WC Forestry SC Forestry GA DZ5MU UT WOS:000385907000011 ER PT J AU Bragg, DC AF Bragg, Don C. TI Initial Mortality Rates and Extent of Damage to Loblolly and Longleaf Pine Plantations Affected by an Ice Storm in South Carolina SO FOREST SCIENCE LA English DT Article DE glaze; Pinus palustris; Pinus taeda; Savannah River Site ID UNITED-STATES; FREEZING RAIN; MANAGEMENT; IMPACTS AB A major ice storm struck Georgia and the Carolinas in February of 2014, damaging or destroying hundreds of thousands of hectares of timber worth hundreds of millions of dollars. Losses were particularly severe in pine plantations in west-central South Carolina, including many on the Savannah River Site (SRS). An array of paired, mid-rotation loblolly (Pinus taeda L.) and longleaf (Pinus palustris Mill.) pine plantations on the SRS provided an opportunity to evaluate species- and size-based tree responses to this storm. A preliminary assessment of these recently thinned plantations found that longleaf pines experienced higher mortality rates than loblolly pines; in part, this result was confounded with tree size (dbh). A more detailed analysis found that longleaf pines, even when controlled for dbh, experienced higher mortality rates and a greater degree of certain types of injuries than comparably sized loblolly pines. These results suggest that longleaf pine planted in glaze-prone regions of the southeastern United States may need to be managed with different planting densities, thinning regimes, and/or rotation ages than loblolly pine. C1 [Bragg, Don C.] US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. RP Bragg, DC (reprint author), US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. EM dbragg@fs.fed.us FU USDA Forest Service's Southern Research Station; Savannah River Site FX I thank the following for their assistance on this project: John Blake, Ed Olson, Steven Butler, and Elizabeth Brooks (all of the USDA Forest Service's Savannah River Site); Timothy Harrington (Pacific Northwest Research Station, USDA Forest Service); James M. Guldin and Nancy Koerth (Southern Research Station, USDA Forest Service); and Tim Slay (consulting forester). Financial support for this project was provided by the USDA Forest Service's Southern Research Station and the Savannah River Site. NR 54 TC 0 Z9 0 U1 4 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD OCT PY 2016 VL 62 IS 5 BP 574 EP 585 DI 10.5849/forsci.15-177 PG 12 WC Forestry SC Forestry GA DZ5MU UT WOS:000385907000012 ER PT J AU Van Ekert, E Chauvigne, F Finn, RN Mathew, LG Hull, JJ Cerda, J Fabrick, JA AF Van Ekert, Evelien Chauvigne, Francois Finn, Roderick Nigel Mathew, Lolita G. Hull, J. Joe Cerda, Joan Fabrick, Jeffrey A. TI Molecular and functional characterization of Bemisia tabaci aquaporins reveals the water channel diversity of hemipteran insects SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Bemisia tabaci; Whitefly; Osmoregulation; Aquaporin; Entomoglyceroporin; Major intrinsic protein family ID BOMBYX-MORI; TRANSMEMBRANE TOPOLOGY; HOMOPTERAN INSECTS; PROTEIN TOPOLOGY; ANTARCTIC MIDGE; FILTER CHAMBER; AEDES-AEGYPTI; CDNA CLONING; EXPRESSION; PREDICTION AB The Middle East-Asia Minor 1 (MEAM1) whitefly, Bemisia tabaci (Gennadius) is an economically important pest of food, fiber, and ornamental crops. This pest has evolved a number of adaptations to overcome physiological challenges, including 1) the ability to regulate osmotic stress between gut lumen and hemolymph after imbibing large quantities of a low nitrogen, sugar-rich liquid diet; 2) the ability to avoid or prevent dehydration and desiccation, particularly during egg hatching and molting; and 3) to be adapted for survival at elevated temperatures. One superfamily of proteins involved in the maintenance of fluid homeostasis in many organisms includes the aquaporins, which are integral membrane channel proteins that aid in the rapid flux of water and other small solutes across biological membranes. Here, we show that B. tabaci has eight aquaporins (BtAqps), of which seven belong to the classical aquaporin 4 related grade of channels, including Bib, Drip, Prip, and Eglps and one that belongs to the unorthodox grade of aquaporin 12-like channels. B. tabaci has further expanded its repertoire of water channels through the expression of three BtDrip2 amino-terminal splice variants, while other hemipteran species express amino- or carboxyl-terminal isoforms of Drip, Prip, and Eglps. Each BtAqp has unique transcript expression profiles, cellular localization, and/or substrate preference. Our phylogenetic and functional data reveal that hemipteran insects lost the classical glp genes, but have compensated for this by duplicating the eglp genes early in their evolution to comprise at least three separate clades of glycerol transporters. Published by Elsevier Ltd. C1 [Van Ekert, Evelien; Mathew, Lolita G.; Hull, J. Joe; Fabrick, Jeffrey A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Chauvigne, Francois; Finn, Roderick Nigel] Univ Bergen, Bergen High Technol Ctr, Dept Biol, N-5020 Bergen, Norway. [Chauvigne, Francois; Cerda, Joan] CSIC, Inst Ciencies Mar, Inst Recerca & Tecnol Agroalimentaries, E-08003 Barcelona, Spain. [Finn, Roderick Nigel] Nordnes, Inst Marine Res, N-5817 Bergen, Norway. RP Fabrick, JA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM jeff.fabrick@ars.usda.gov RI Cerda, Joan/M-1099-2016; Chauvigne, Francois/D-6854-2017 OI Cerda, Joan/0000-0003-2568-6398; Chauvigne, Francois/0000-0001-5571-7517 FU base CRIS; National Program - Crop Protection Quarantine [304, 2020-22620-022-00D]; Research Council of Norway [204813/F20, 254872/E40]; Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2013-41196-R]; USDA-ARS Research Associate Program FX This work was supported by base CRIS funding to USDA-ARS, National Program 304 - Crop Protection & Quarantine [Project #2020-22620-022-00D)] to J.F., and partially funded by the Research Council of Norway Grants 204813/F20 and 254872/E40 to R.N.F. and the Spanish Ministry of Economy and Competitiveness (MINECO), project AGL2013-41196-R to J.C. E.V.E. was supported by the USDA-ARS Research Associate Program to J.F. NR 81 TC 0 Z9 0 U1 11 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD OCT PY 2016 VL 77 BP 39 EP 51 DI 10.1016/j.ibmb.2016.07.010 PG 13 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DY7PN UT WOS:000385321700005 PM 27491441 ER PT J AU Polejaeva, IA Ranjan, R Davies, CJ Regouski, M Hall, J Olsen, AL Meng, QG Rutigliano, HM Dosdall, DJ Angel, NA Sachse, FB Seidel, T Thomas, AJ Stott, R Panter, KE Lee, PM Van Wettere, AJ Stevens, JR Wang, ZD Macleod, RS Marrouche, NF White, KL AF Polejaeva, Irina A. Ranjan, Ravi Davies, Christopher J. Regouski, Misha Hall, Justin Olsen, Aaron L. Meng, Qinggang Rutigliano, Heloisa M. Dosdall, Derek J. Angel, Nathan A. Sachse, Frank B. Seidel, Thomas Thomas, Aaron J. Stott, Rusty Panter, Kip E. Lee, Pamela M. Van Wettere, Arnaud J. Stevens, John R. Wang, Zhongde Macleod, Rob S. Marrouche, Nassir F. White, Kenneth L. TI Increased Susceptibility to Atrial Fibrillation Secondary to Atrial Fibrosis in Transgenic Goats Expressing Transforming Growth Factor-1 SO JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY LA English DT Article DE atrial fibrillation; fibrosis; genetics; TGF-1 transgenic goat model ID HEART-FAILURE; STRUCTURAL ABNORMALITIES; QUANTITATIVE-ANALYSIS; CARDIAC FIBROSIS; NUCLEAR TRANSFER; CANINE MODEL; MECHANISMS; ABLATION; FIBROBLASTS; CONDUCTION AB AF Susceptibility in TGF-1 Transgenic Goats IntroductionLarge animal models of progressive atrial fibrosis would provide an attractive platform to study relationship between structural and electrical remodeling in atrial fibrillation (AF). Here we established a new transgenic goat model of AF with cardiac specific overexpression of TGF-1 and investigated the changes in the cardiac structure and function leading to AF. Methods and ResultsTransgenic goats with cardiac specific overexpression of constitutively active TGF-1 were generated by somatic cell nuclear transfer. We examined myocardial tissue, ECGs, echocardiographic data, and AF susceptibility in transgenic and wild-type control goats. Transgenic goats exhibited significant increase in fibrosis and myocyte diameters in the atria compared to controls, but not in the ventricles. P-wave duration was significantly greater in transgenic animals starting at 12 months of age, but no significant chamber enlargement was detected, suggesting conduction slowing in the atria. Furthermore, this transgenic goat model exhibited a significant increase in AF vulnerability. Six of 8 transgenic goats (75%) were susceptible to AF induction and exhibited sustained AF (>2 minutes), whereas none of 6 controls displayed sustained AF (P < 0.01). Length of induced AF episodes was also significantly greater in the transgenic group compared to controls (687 212.02 seconds vs. 2.50 +/- 0.88 seconds, P < 0.0001), but no persistent or permanent AF was observed. ConclusionA novel transgenic goat model with a substrate for AF was generated. In this model, cardiac overexpression of TGF-1 led to an increase in fibrosis and myocyte size in the atria, and to progressive P-wave prolongation. We suggest that these factors underlie increased AF susceptibility. C1 [Polejaeva, Irina A.; Davies, Christopher J.; Regouski, Misha; Hall, Justin; Olsen, Aaron L.; Meng, Qinggang; Rutigliano, Heloisa M.; Thomas, Aaron J.; Stott, Rusty; Van Wettere, Arnaud J.; Wang, Zhongde; White, Kenneth L.] Utah State Univ, Dept Anim Dairy & Vet Sci, 4815 Old Main Hill, Logan, UT 84322 USA. [Panter, Kip E.] USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84321 USA. [Ranjan, Ravi; Angel, Nathan A.; Macleod, Rob S.; Marrouche, Nassir F.] Univ Utah, Div Cardiol, CARMA Ctr, Salt Lake City, UT 84112 USA. [Ranjan, Ravi; Sachse, Frank B.] Univ Utah, Dept Bioengn, Salt Lake City, UT 84112 USA. [Dosdall, Derek J.] Univ Utah, Ctr Engn Innovat, Salt Lake City, UT USA. [Sachse, Frank B.; Seidel, Thomas; Macleod, Rob S.] Univ Utah, Nora Eccles Harrison Cardiovasc Res & Training In, Salt Lake City, UT USA. [Lee, Pamela M.] Washington State Univ, Coll Vet Med, Pullman, WA 99164 USA. [Stevens, John R.] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. RP Polejaeva, IA (reprint author), Utah State Univ, Dept Anim Dairy & Vet Sci, 4815 Old Main Hill, Logan, UT 84322 USA. EM irina.polejaeva@usu.edu FU Utah Science Technology and Research agency; American Heart Association [13GRNT16850082]; National Institute of Health [K23HL115084, R01HL128752]; Utah Agricultural Experimental Station; Nora Eccles Treadwell Foundation; [8786] FX This work was supported by a grant from the Utah Science Technology and Research agency provided to the Utah Multidisciplinary Arrhythmia Consortium (to I.A.P., R.R., C.J.D., D.J.D., R.S.M., N.M., A.L.O., and K.L.W.); the American Heart Association (13GRNT16850082 to I.A.P.); and the National Institute of Health (K23HL115084 to R.R. and R01HL128752 to D.J.D). This research was also supported by the Utah Agricultural Experimental Station (to I.A.P.) and approved as journal paper #8786. We also acknowledge funding by the Nora Eccles Treadwell Foundation (to F.B.S. and T.S). NR 46 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1045-3873 EI 1540-8167 J9 J CARDIOVASC ELECTR JI J. Cardiovasc. Electrophysiol. PD OCT PY 2016 VL 27 IS 10 BP 1220 EP 1229 DI 10.1111/jce.13049 PG 10 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA DZ4PN UT WOS:000385840900014 PM 27447370 ER PT J AU Perez-Martinez, P Alcala-Diaz, JF Kabagambe, EK Garcia-Rios, A Tsai, MY Delgado-Lista, J Kolovou, G Straka, RJ Gomez-Delgado, F Hopkins, PN Marin, C Borecki, I Yubero-Serrano, EM Hixson, JE Camargo, A Province, MA Lopez-Moreno, J Rodriguez-Cantalejo, F Tinahones, FJ Mikhailidis, DP Perez-Jimenez, F Arnett, DK Ordovas, JM Lopez-Miranda, J AF Perez-Martinez, Pablo Alcala-Diaz, Juan F. Kabagambe, Edmon K. Garcia-Rios, Antonio Tsai, Michael Y. Delgado-Lista, Javier Kolovou, Genovefa Straka, Robert J. Gomez-Delgado, Francisco Hopkins, Paul N. Marin, Carmen Borecki, Ingrid Yubero-Serrano, Elena M. Hixson, James E. Camargo, Antonio Province, Michael A. Lopez-Moreno, Javier Rodriguez-Cantalejo, Fernando Tinahones, Francisco J. Mikhailidis, Dimitri P. Perez-Jimenez, Francisco Arnett, Donna K. Ordovas, Jose M. Lopez-Miranda, Jose TI Assessment of postprandial triglycerides in clinical practice: Validation in a general population and coronary heart disease patients SO JOURNAL OF CLINICAL LIPIDOLOGY LA English DT Article DE Triglycerides; Postprandial lipemia; Oral-fat tolerance test; Coronary heart disease; CORDIOPREV study; GOLDN study ID CARDIOVASCULAR-DISEASE; NONFASTING TRIGLYCERIDES; LIPOPROTEIN METABOLISM; RICH LIPOPROTEINS; PATERNAL HISTORY; LIPID-METABOLISM; ARTERY-DISEASE; RISK; LIPEMIA; PLASMA AB BACKGROUND: Previous studies have suggested that for clinical purposes, subjects with fasting triglycerides (TGs) between 89-180 mg/dl (1-2 mmol/l) would benefit from postprandial TGs testing. OBJECTIVE: To determine the postprandial TG response in 2 independent studies and validate who should benefit diagnostically from an oral-fat tolerance test (OFTT) in clinical practice. METHODS: A population of 1002 patients with coronary heart disease (CHD) from the CORDIOPREV clinical trial and 1115 white US subjects from the GOLDN study underwent OFTTs. Subjects were classified into 3 groups according to fasting cut points of TGs to predict the usefulness of OFTT: (1) TG < 89 mg/dl (<1 mmol/l); (2) TG, 89-180 mg/dl (1-2 mmol/l); and (3) TG > 180 mg/dl (>2 mmol/l). Postprandial TG concentration at any point > 220 mg/dl (>2.5 mmol/l) has been preestablished as an undesirable postprandial response. RESULTS: Of the total, 49% patients with CHD and 42% from the general population showed an undesirable response after the OFTT. The prevalence of undesirable postprandial TG in the CORDIOPREV clinical trial was 12.8, 50.3, and 89.7%, in group 1, 2, and 3, respectively (P <.001) and 11.2, 58.1, and 97.5% in group 1, 2, and 3, respectively (P <.001) in the GOLDN study. CONCLUSIONS: These two studies validate the predictive values reported in a previous consensus. Moreover, the findings of the CORDIOPREV and GOLDN studies show that an OFF! is useful to identify postprandial hyperlipidemia in subjects with fasting TG between 1-2 mmol/l (89-180 mg/dL), because approximately half of them have hidden postprandial hyperlipidemia, which may influence treatment. An OFTT does not provide additional information regarding postprandial hyperlipidemia in subjects with low TG (<1 mmolll, <89 mg/dL) or increased TG (>2 mmol/l, >180 mg/dl). (C) 2016 National Lipid Association. All rights reserved. C1 [Perez-Martinez, Pablo; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Delgado-Lista, Javier; Gomez-Delgado, Francisco; Marin, Carmen; Yubero-Serrano, Elena M.; Camargo, Antonio; Lopez-Moreno, Javier; Perez-Jimenez, Francisco; Lopez-Miranda, Jose] Univ Cordoba, Reina Sofia Univ Hosp, IMIBIC, Lipid & Atherosclerosis Unit,Dept Internal Med, Cordoba, Spain. [Perez-Martinez, Pablo; Alcala-Diaz, Juan F.; Garcia-Rios, Antonio; Delgado-Lista, Javier; Gomez-Delgado, Francisco; Marin, Carmen; Yubero-Serrano, Elena M.; Camargo, Antonio; Lopez-Moreno, Javier; Tinahones, Francisco J.; Perez-Jimenez, Francisco; Lopez-Miranda, Jose] Inst Salud Carlos III, GIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain. [Kabagambe, Edmon K.] Vanderbilt Univ, Med Ctr, Dept Med, Div Epidemiol, Nashville, TN USA. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Kolovou, Genovefa] Onassis Cardiac Surg Ctr, Cardiol Dept 1, Athens, Greece. [Straka, Robert J.] Univ Minnesota, Coll Pharm, Dept Expt & Clin Pharmacol, Minneapolis, MN 55455 USA. [Hopkins, Paul N.] Univ Utah, Hlth Sci Ctr, Dept Internal Med, Salt Lake City, UT USA. [Borecki, Ingrid] Washington Univ, Ctr Genome Sci, Div Stat Genom, St Louis, MO USA. [Hixson, James E.] Univ Texas Hlth Sci Ctr Houston, Ctr Human Genet, Houston, TX 77030 USA. [Province, Michael A.] Washington Univ, Sch Med, Dept Genet, Div Stat Genom, St Louis, MO 63110 USA. [Rodriguez-Cantalejo, Fernando] Hosp Univ Reina Sofia, Biochem Lab, Cordoba, Spain. [Tinahones, Francisco J.] Hosp Clin Virgen de la Victoria, Serv Endocrinol & Nutr, Malaga, Spain. [Mikhailidis, Dimitri P.] UCL, Sch Med, Dept Clin Biochem, Royal Free Hosp Campus, London, England. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Ordovas, Jose M.] Tufts Univ, Sch Med, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [Ordovas, Jose M.] Natl Ctr Cardiovasc Invest, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Madrid Inst Adv Studies Food, Madrid, Spain. RP Perez-Martinez, P (reprint author), Reina Sofia Univ Hosp, Lipids & Atherosclerosis Res Unit, Avda Menendez Pidal S-N, Cordoba 14004, Spain. EM pablopermar@yahoo.es RI Yubero-Serrano, Elena /H-4832-2013 FU Fundacion Patrimonio Comunal Olivarero; CITOLIVA; CEAS; Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa); Diputaciones de Jaen y Cordoba; Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Ministerio de Medio Ambiente, Medio Rural y Marino, Spanish Government; Ministerio de Ciencia e Innovacion [AGL2009-122270, FIS PI13/00185, FIS PI13/00023]; Ministerio de Economia y Competitividad [AGL2012/39615]; Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo [CVI-7450]; European Community (NUTRITECH European Integrated Project) [289511]; NIH Heart,Lung and Blood Institute [U 01 HL72524]; Genetic and Environmental Determinants of Triglycerides; ISCIII research contract (Programa Rio-Hortega) FX The CORDIOPREV study is supported by the Fundacion Patrimonio Comunal Olivarero. We also received additional funding from CITOLIVA, CEAS, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud, and Ministerio de Medio Ambiente, Medio Rural y Marino, Spanish Government. It was also partly supported by research grants from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to Jose Lopez-Miranda, FIS PI13/00185 to Dr Perez-Martinez, FIS PI13/00023 to Dr Delgado-Lista); Ministerio de Economia y Competitividad (AGL2012/39615 to Jose Lopez-Miranda); Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo (CVI-7450 to Jose Lopez-Miranda); by a Research Grant from the European Community (NUTRITECH European Integrated Project-289511). The GOLDN study was supported by NIH Heart,Lung and Blood Institute grant U 01 HL72524, Genetic and Environmental Determinants of Triglycerides. Dr Gomez-Delgado is supported by an ISCIII research contract (Programa Rio-Hortega). The CIBER-OBN is an initiative of the Institute de Salud Carlos Madrid, Spain. NR 35 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1933-2874 EI 1876-4789 J9 J CLIN LIPIDOL JI J. Clin. Lipidol. PD OCT PY 2016 VL 10 IS 5 BP 1163 EP 1171 DI 10.1016/j.jacl.2016.05.009 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DZ5LN UT WOS:000385903700014 PM 27678433 ER PT J AU Hajek, AE Gryganskyi, A Bittner, T Liebherr, JK Liebherr, JH Jensen, AB Moulton, JK Humber, RA AF Hajek, Ann E. Gryganskyi, Andrii Bittner, Tonya Liebherr, James K. Liebherr, Jonathan H. Jensen, Annette Bruun Moulton, John K. Humber, Richard A. TI Phylogenetic placement of two species known only from resting spores: Zoophthora independentia sp nov and Z. porteri comb nov (Entomophthorales: Entomophthoraceae) SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Entomopathogenic fungi; Tipula; Entomophthorales; Resting spores ID FORMATION IN-VIVO; NESTED-PCR; FUNGI; COMPLEX; GENUS; RDNA AB Molecular methods were used to determine the generic placement of two species of Entomophthorales known only from resting spores. Historically, these species would belong in the form-genus Tarichium, but this classification provides no information about phylogenetic relationships. Using DNA from resting spores, Zoophthora independentia, infecting Tipula (Lunatipula) submaculata in New York State, is now described as a new species and Tarichium porteri, described in 1942, which infects Tipula(Triplicitipula) colei in Tennessee, is transferred to the genus Zoophthora. We have shown that use of molecular methods can assist with determination of the phylogenetic relations of specimens within the form-genus Tarichium for an already described species and a new species for which only resting spores are available. (C) 2016 Elsevier Inc. All rights reserved. C1 [Hajek, Ann E.; Bittner, Tonya; Liebherr, James K.; Liebherr, Jonathan H.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Gryganskyi, Andrii] Duke Univ, Dept Biol, Durham, NC 27708 USA. [Jensen, Annette Bruun] Univ Copenhagen, Dept Agr & Ecol, DK-1871 Frederiksberg, Denmark. [Moulton, John K.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Humber, Richard A.] ARS, USDA, Robert W Holley Ctr, Tower Rd, Ithaca, NY 14853 USA. RP Hajek, AE (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM aeh4@cornell.edu RI Jensen, Annette/G-7558-2014 OI Jensen, Annette/0000-0002-2044-2274 NR 34 TC 0 Z9 0 U1 4 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD OCT PY 2016 VL 140 BP 68 EP 74 DI 10.1016/j.jip.2016.09.002 PG 7 WC Zoology SC Zoology GA DZ5ML UT WOS:000385906100011 PM 27637932 ER PT J AU Geng, P Sun, JH Zhang, ML Li, XN Harnly, JM Chen, P AF Geng, Ping Sun, Jianghao Zhang, Mengliang Li, Xingnuo Harnly, James M. Chen, Pei TI Comprehensive characterization of C-glycosyl flavones in wheat (Triticum aestivum L.) germ using UPLC-PDA-ESI/HRMSn and mass defect filtering SO JOURNAL OF MASS SPECTROMETRY LA English DT Article DE di-C-glycosyl flavones; O-acylated-C-glycosyl flavones; mass defect filter; wheat germ; high resolution mass spectrometry ID PERFORMANCE LIQUID-CHROMATOGRAPHY; FAST-ATOM-BOMBARDMENT; ELECTROSPRAY-IONIZATION; STRUCTURAL-CHARACTERIZATION; SPECTROMETRY; LEAVES; DIFFERENTIATION; FRAGMENTATION; MS; IDENTIFICATION AB A comprehensive characterization of C-glycosyl flavones in wheat germ has been conducted using multi-stage high resolution mass spectrometry (HRMSn) in combination with a mass defect filtering (MDF) technique. MDF performed the initial search of raw data with defined C-glycosyl flavone mass windows and mass defect windows to generate the noise-reduced data focusing on targeted flavonoids. The high specificity of the exact mass measurement permits the unambiguous discrimination of acyl groups (nominal masses of 146, 162 and 176.) from sugar moieties (rhamnose, glucose or galactose and glucuronic acid). A total of 72 flavone C-glycosyl derivatives, including 2 mono-C-glycosides, 34 di-C-glycosides, 15 tri-glycosides, 14 acyl di-C-glycosides and 7 acyl tri-glycosides, were characterized in wheat germ, some of which were considered to be important marker compounds for differentiation of whole grain and refined wheat products. The 7 acylated mono-O-glycosyl-di-C-glycosyl flavones and some acylated di-C-glycosyl flavones are reported in wheat for the first time. The frequent occurrence of numerous isomers is a remarkable feature of wheat germ flavones. Both UV and mass spectra are needed to maximize the structure information obtained for data interpretation. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Geng, Ping; Sun, Jianghao; Zhang, Mengliang; Li, Xingnuo; Harnly, James M.; Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Li, Xingnuo] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China. RP Chen, P (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA,BARC East, Bldg 161,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov RI Li, Xiaonian/G-2026-2011 FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 42 TC 0 Z9 0 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1076-5174 EI 1096-9888 J9 J MASS SPECTROM JI J. Mass Spectrom. PD OCT PY 2016 VL 51 IS 10 BP 914 EP 930 DI 10.1002/jms.3803 PG 17 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA DZ4UI UT WOS:000385855400007 PM 27373213 ER PT J AU Bai, Y Gilbert, A Fox, K Osikowicz, L Kosoy, M AF Bai, Ying Gilbert, Amy Fox, Karen Osikowicz, Lynn Kosoy, Michael TI BARTONELLA ROCHALIMAE AND B. VINSONII SUBSP BERKHOFFII IN WILD CARNIVORES FROM COLORADO, USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Bartonella vinsonii subsp berkhoffii; Bartonella rochalimae; Colorado; wild carnivores; wildlife diseases ID CITRATE SYNTHASE GENE; PHYLOGENETIC ANALYSIS; GRAY FOXES; CALIFORNIA; INFECTION; SEQUENCES; DOGS; DIFFERENTIATION; ENDOCARDITIS; RESERVOIR AB Spleen samples from 292 wild carnivores from Colorado, US were screened for Bartonella infection. Bartonella DNA was detected in coyotes (Canis latrans) (28%), striped skunks (Mephitis mephitis) (23%), red foxes (Vulpes vulpes) (27%), and raccoons (Procyon lotor) (8%) but not in black bears (Ursus americanus), gray foxes (Urocyon cinereoargenteus), and mountain lions (Puma concolor). Two Bartonella species, B. vinsonii subsp. berkhoffii and B. rochalimae, were identified. All 10 infected striped skunks exclusively carried B. rochalimae while coyotes, red foxes, and raccoons could be infected with both Bartonella species. Five of seven infected coyotes carried B. v. berkhoffii whereas five of seven infected red foxes and 11 of 14 infected raccoons carried B. rochalimae. Further studies are needed to understand relationships between Bartonella species, wild carnivores, and their ectoparasites. C1 [Bai, Ying; Osikowicz, Lynn; Kosoy, Michael] Ctr Dis Control & Prevent, Bacterial Dis Branch, Div Vector Borne Dis, 3156 Rampart Rd, Ft Collins, CO 80521 USA. [Gilbert, Amy] APHIS, Natl Wildlife Res Ctr, USDA, Wildlife Serv, 4101 Laporte Ave, Ft Collins, CO 80521 USA. [Fox, Karen] Colorado Pk & Wildlife, 317 W Prospect Rd, Ft Collins, CO 80525 USA. RP Bai, Y (reprint author), Ctr Dis Control & Prevent, Bacterial Dis Branch, Div Vector Borne Dis, 3156 Rampart Rd, Ft Collins, CO 80521 USA. EM bby5@cdc.gov NR 26 TC 0 Z9 0 U1 3 U2 3 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2016 VL 52 IS 4 BP 844 EP 849 DI 10.7589/2016-01-015 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DZ4RG UT WOS:000385846300009 PM 27529290 ER PT J AU Rhyan, J McCollum, M Gidlewski, T Shalev, M Ward, G Donahue, B Arzt, J Stenfeldt, C Mohamed, F Nol, P Deng, M Metwally, S McKenna, T Salman, M AF Rhyan, Jack McCollum, Matthew Gidlewski, Thomas Shalev, Moshe Ward, Gordon Donahue, Brenda Arzt, Jonathan Stenfeldt, Carolina Mohamed, Fawzi Nol, Pauline Deng, Ming Metwally, Samia McKenna, Thomas Salman, Mo TI FOOT-AND-MOUTH DISEASE IN A SMALL SAMPLE OF EXPERIMENTALLY INFECTED PRONGHORN (ANTILOCAPRA AMERICANA) SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Antilocapra americana; foot-and-mouth disease; FMD; pathology; PCR; pronghorn; ungulate; wildlife ID BRITISH DEER; VIRUS; TRANSMISSION; WILDLIFE; DIFFERENTIATION; BACULOVIRUS; ANTIBODIES; AFRICA; 3ABC AB There is limited information on the pathogenesis and epidemiology of foot-and-mouth disease (FMD) in North American wildlife and none concerning pronghorn (Antilocapra americana). In an experimental study of 13 pronghorn and six steers (Bos taurus), we compared the susceptibility of pronghorn to FMD virus (FMDV) strain O, with that of cattle (Bos taurus). We also determined the potential for intra-and interspecies transmission of FMDV strain O in pronghorn and cattle, assessed the application of conventional laboratory tests in their suitability to detect FMDV infection in pronghorn, and evaluated the potential role of pronghorn as efficient long-term carriers of FMDV. After acclimation to containment at Plum Island Animal Disease Center, two pronghorn and one steer were each infected by intraepithelial tongue inoculation with 10,000 bovine tongue infective doses of FMDV, strain O1 Manisa. Inoculated animals were housed with contact animals. When contact-exposed animals developed fever they were placed in rooms with previously unexposed animals. All inoculated and exposed cattle and pronghorn developed clinical disease typical of FMD. Pronghorn developed severe foot lesions and mild to moderate oral lesions, primarily on the tongue. Duration of clinical signs in both species was 2-3 wk with foot abnormalities evident to the end of the study (51 d postexposure). Other lesions included pancreatitis, myositis of the tongue, and secondary lesions including pleuritis, pneumonia, decubital ulcers, and tenosynovitis. Virus transmission occurred between pronghorn, from cattle to pronghorn, and from pronghorn to cattle. Conventional laboratory tests detected virus and antibodies against nonstructural and structural FMDV proteins in pronghorn and cattle. Virus was present in some animals for 1 wk but was not detectable by virus isolation or PCR at 3 wk postinfection or afterward. C1 [Rhyan, Jack; McCollum, Matthew; Gidlewski, Thomas; Nol, Pauline] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Shalev, Moshe] Plum Isl Anim Dis Ctr, Dept Homeland Secur, Long Isl City, NY 11957 USA. [Ward, Gordon; Donahue, Brenda; Mohamed, Fawzi; Deng, Ming; Metwally, Samia; McKenna, Thomas] US Anim & Plant Hlth Inspect Serv, Foreign Anim Dis Diagnost Lab, USDA, Long Isl City, NY 11957 USA. [Arzt, Jonathan; Stenfeldt, Carolina] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Long Isl City, NY 11944 USA. [Stenfeldt, Carolina] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN 37831 USA. [Salman, Mo] Colorado State Univ, Coll Vet Med & Biomed Sci, 300 W Drake Rd, Ft Collins, CO 80523 USA. [Metwally, Samia] UN, Food & Agr Org, Viale Terme Carracalla, I-00153 Rome, Italy. [McKenna, Thomas] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, 160 Worcester Providence Rd, Sutton, MA 01590 USA. RP Rhyan, J (reprint author), US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM jack.c.rhyan@aphis.usda.gov OI Arzt, Jonathan/0000-0002-7517-7893 FU US Department of Agriculture (USDA); National Institute of Food and Agriculture (NIFA) through National Research Initiative; Colorado State University Program of Economically Important Infectious Animal Diseases - USDA: NIFA FX We thank Temple Grandin, Mark Deesing, Lauren Harris, and Melissa Syndergaard for excellent assistance in acclimating pronghorn to captivity and handling, and Jeffrey Babcock and his dedicated Animal Care Staff at PIADC for their exceptional handling and care of these challenging animals in biocontainment. Luis Rodriguez and his staff at PIADC, Dave Miller. Elizabeth Clark, and Kathleen Apicelli, provided valuable technical assistance to the study. This study was partially supported by a grant from US Department of Agriculture (USDA): National Institute of Food and Agriculture (NIFA; formerly USDA: Cooperative State Research, Education, and Extension Service) through National Research Initiative and the Colorado State University Program of Economically Important Infectious Animal Diseases funded by a special grant from USDA: NIFA. NR 23 TC 0 Z9 0 U1 1 U2 1 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2016 VL 52 IS 4 BP 862 EP 873 DI 10.7589/2015-11-312 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA DZ4RG UT WOS:000385846300011 PM 27525593 ER PT J AU Ferguson, SD Wellehan, JFX Frasca, S Innis, CJ Harris, HS Miller, M Weber, ES Walden, HS Greiner, EC Merigo, C Stacy, BA AF Ferguson, Sara D. Wellehan, James F. X., Jr. Frasca, Salvatore, Jr. Innis, Charles J. Harris, Heather S. Miller, Melissa Weber, E. Scott Walden, Heather Stockdale Greiner, Ellis C. Merigo, Constance Stacy, Brian A. TI COCCIDIAL INFECTION OF THE ADRENAL GLANDS OF LEATHERBACK SEA TURTLES (DERMOCHELYS CORIACEA) SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Eimeriidae; mortality; oocysts; protozoa; qPCR; 18S rRNA; sea turtle ID ELECTRON-MICROSCOPY; INTRANUCLEAR COCCIDIOSIS; CHELONIA-MYDAS; TESTUDINES; MICROWAVE; DIAGNOSIS; BOOTSTRAP; TORTOISES AB Histologic lesions incidental to the cause of death were observed in the adrenal glands of 17 subadult and adult leatherback sea turtles (Dermochelys coriacea) found dead or moribund on or near shore in North America. Round bodies, 250-300 mu m in diameter composed of an outer capsule and large multinucleated cells surrounding a central mass of acellular material were distributed throughout the affected glands. Protozoal etiology was suspected based on some resemblance to coccidia; however, features diagnostic for coccidial infection were lacking in all but one case, which had a focal area of adrenalitis containing zoites. A novel eucoccidian partial 18S rRNA genetic sequence was consistently detected in adrenal glands with lesions. With the use of quantitative PCR, a specific area of the V4 region of the coccidian 18S gene was quantified in affected adrenal glands and correlated significantly with density of the histologic lesions. A second distinct, but closely related, 18S sequence was also amplified from the adrenal gland of one turtle and from a fecal sample containing unsporulated coccidian oocysts. The two 18S sequences identified from leatherback sea turtles form a clade within the family Eimeriidae. Further investigation is required to understand better the morphology of the life stages, life cycle, and potential effects of this coccidian parasite on adrenal function. C1 [Ferguson, Sara D.; Wellehan, James F. X., Jr.; Walden, Heather Stockdale; Greiner, Ellis C.] Univ Florida, Coll Vet Med, POB 110126,2015 SW 16th Ave, Gainesville, FL 32608 USA. [Frasca, Salvatore, Jr.] Univ Connecticut, Connecticut Vet Med Diagnost Lab, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. [Innis, Charles J.; Weber, E. Scott; Merigo, Constance] New England Aquarium, Cent Wharf, Boston, MA 02110 USA. [Harris, Heather S.] Natl Marine Fisheries Serv, Southwest Fisheries Sci Ctr, 8901 La Jolla Shores Dr, La Jolla, CA 92037 USA. [Miller, Melissa] Marine Wildlife Vet Care & Res Ctr, Calif Dept Fish & Wildlife, Off Spill Prevent & Response, 1451 Shaffer Rd, Santa Cruz, CA 95060 USA. [Stacy, Brian A.] Univ Florida, Natl Marine Fisheries Serv, Natl Ocean & Atmospher Adm, POB 110885,2187 Mowry Rd, Gainesville, FL 32611 USA. [Weber, E. Scott] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, 400 N 8th St,Suite 726, Richmond, VA 23219 USA. RP Stacy, BA (reprint author), Univ Florida, Natl Marine Fisheries Serv, Natl Ocean & Atmospher Adm, POB 110885,2187 Mowry Rd, Gainesville, FL 32611 USA. EM Brian.Stacy@noaa.gov FU Morris Animal Foundation Summer Student Scholar Program; US Department of Commerce, National Oceanic and Atmospheric Administration, National Marine Fisheries Service [NA10NMF4720028] FX We thank participants in the Sea Turtle Stranding and Salvage Network (US) and the Canadian Sea Turtle Network (Kathleen Martin), Department of Fisheries and Oceans Canada, Population Ecology Division (Mike James and officers of the Conservation and Protection Division), Florida Fish and Wildlife Conservation Commission, staff and volunteers of New England Aquarium, the Marine Mammal Center, :Moss Landing Marine Laboratories Marine Operations, Jennifer Keene, Robert Prescott, Kara Dodge, Erin Burke, Michael Dodge, Mark Leach, George Purmont, Molly Lutcavage, Sea Rogers Williams, Bridget Dunnigan, Don Lewis, Michael Moore, Darlene Ketten, Betty Lintell, Kate Sampson, Scott Benson, Craig Harms, Emily Christiansen, Sue Barco, Matthew Godfrey, International Fund for Animal Welfare, Massachusetts Department of Marine Fisheries, Massachusetts Audubon Society, Provincetown Center for Coastal Studies, Paul Doshkov and staff of the Cape Hatteras National Seashore, and the California Department of Fish and Wildlife, We thank Chris Gardiner for review of earlier manuscript drafts and Stephen Daniels for the electron microscopy services. S.D.F. was supported by the Morris Animal Foundation Summer Student Scholar Program. Partial funding was provided by US Department of Commerce, National Oceanic and Atmospheric Administration, National Marine Fisheries Service (NA10NMF4720028). Live turthe capture was conducted under the authority of the National Marine Fisheries Service Endangered Species Act Section 10 Permit 15672, and approved by the Institutional Animal Care and Use Committees of the Ness England Aquarium (06-03) and the University of Massachusetts (2010-0019). NR 25 TC 1 Z9 1 U1 10 U2 10 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2016 VL 52 IS 4 BP 874 EP 882 DI 10.7589/2015-11-310 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA DZ4RG UT WOS:000385846300012 PM 27455196 ER PT J AU Johnson, SR Crider, NJ Weyer, GA Tosh, RD VerCauteren, KC AF Johnson, Shylo R. Crider, Nikki J. Weyer, Grant A. Tosh, Randall D. VerCauteren, Kurt C. TI BAIT DEVELOPMENT FOR ORAL DELIVERY OF PHARMACEUTICALS TO RACCOONS (PROCYON LOTOR) AND STRIPED SKUNKS (MEPHITIS MEPHITIS) SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Bait; oral vaccination; raccoon; rabies; skunk ID RABIES VACCINE; UNITED-STATES; ATTRACTANTS; RESPONSES; COYOTES; ONTARIO; CANADA AB Oral vaccination is one tool used to control wildlife diseases. A challenge to oral vaccination is identifying baits specific to target species. The US has been conducting oral vaccination against rabies since the 1990s. Improvements in bait development will hasten disease elimination. In Colorado, we examined a novel bait for oral vaccination and offered two different flavors, sweet and fish, to captive raccoons (Procyon lotor) and striped skunks (Mephitis mephitis) to assess consumption and flavor preference and observed bait removal by target and nontarget species in the field. During captive trials, raccoons and skunks consumed 98% and 87% of offered baits, respectively. Baits contained a sachet to simulate a vaccine package. Raccoons and skunks consumed 98% and 94% of the sachets, respectively. All unconsumed sachets were punctured, suggesting that animals had oral exposure to the contents. Raccoons preferred fish-flavored bait, but skunks did not have a preference. In the field, raccoons consumed the most baits, followed by fox squirrels (Sciurus niger). Other rabies host species (striped skunks, red foxes [Vulpes vulpes], coyotes [Canis latrans]) had very low visitation and were never observed consuming baits. High consumption rates by raccoons and skunks in captivity and observance of raccoons consuming baits in the field suggest that these baits may be useful for oral delivery of pharmaceuticals. Further field research is warranted to determine how to best optimize bait delivery. C1 [Johnson, Shylo R.; Crider, Nikki J.; VerCauteren, Kurt C.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Weyer, Grant A.; Tosh, Randall D.] SmartVet Holdings Inc, 22201 W Innovat Dr,170A, Olathe, KS 66061 USA. RP Johnson, SR (reprint author), US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM shylo.r.johnson@aphis.usda.gov FU NWRC FX We thank everyone that assisted in acquiring animals, setting up bait stations and cameras, providing computer and camera technical help, and caring for the animals, especially T. Rigg, C. Ellis, R. Moulton, J. Fischer, and J. Haley. Special thanks to the businesses and private landowners that granted access to land. The baits tested were manufactured by Smart Vet USA Inc., International Animal Health and Food Safety Institute, and this company provided funding for acquiring animals and technician support. All other funding was provided by the NWRC. NR 34 TC 0 Z9 0 U1 7 U2 7 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD OCT PY 2016 VL 52 IS 4 BP 893 EP 901 DI 10.7589/2015-12-322 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA DZ4RG UT WOS:000385846300014 PM 27505038 ER PT J AU Agga, GE Schmidt, JW Arthur, TM AF Agga, Getahun E. Schmidt, John W. Arthur, Terrance M. TI Antimicrobial-Resistant Fecal Bacteria from Ceftiofur-Treated and Nonantimicrobial-Treated Comingled Beef Cows at a Cow-Calf Operation SO MICROBIAL DRUG RESISTANCE LA English DT Article DE antimicrobial resistance; ceftiofur; beef cows; enterococcus; E; coli; Salmonella; cephalosporin ID POLYMERASE-CHAIN-REACTION; ESCHERICHIA-COLI; DAIRY-CATTLE; SALMONELLA-ENTERICA; FEEDLOT CATTLE; COPPER SUPPLEMENTATION; UNITED-STATES; WEANED PIGS; CHLORTETRACYCLINE; PREVALENCE AB We compared the occurrences of 3rd-generation cephalosporin-resistant (3GC(r)), tetracycline-resistant (TETr), and trimethoprim-sulfamethoxazole-resistant (COTr) Escherichia coli, 3GC(r) and nalidixic acid-resistant (NAL(r)) Salmonella enterica, and erythromycin-resistant (ERYr) enterococci from the fecal samples of ceftiofur-treated (n=162) and nonantimicrobial-treated (n=207) comingled beef cows 8 years old, for which complete antimicrobial treatment records were available. The prevalence of 3GC(r) (17%; n=369), TETr (88%), (COTE)-E-r. coli (22%), and ERYr enterococci (69%) was not significantly (p>0.05) associated with ceftiofur treatment, prior history of other antimicrobial treatments, or duration of time between last antimicrobial treatment and sampling. 3GC(r) and NAL(r)S. enterica were not detected. The prevalence of tetB was significantly (p<0.05) higher compared with tetA among (TETE)-E-r. coli. However, the prevalence of tetA was significantly (p<0.05) higher than tetB among 3GC(r) and (COTE)-E-r. coli. There was a significant (p<0.05) association between tetM and ermB among ERYr enterococci. In conclusion, occurrences of 3GC(r), TETr, and (COTE)-E-r. coli and ERYr enterococci in comingled antimicrobial-treated and nonantimicrobial-treated beef cows were not associated with ceftiofur or other antimicrobial use, indicating that other factors influenced the observed levels of antimicrobial-resistant bacteria in feces of beef cows. C1 [Agga, Getahun E.; Schmidt, John W.; Arthur, Terrance M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Arthur, TM (reprint author), ARS, USDA, US Meat Anim Res Ctr, Meat Safety & Qual Res Unit, POB 166,State Spur 18 D, Clay Ctr, NE 68933 USA. EM terrance.arthur@ars.usda.gov NR 39 TC 0 Z9 0 U1 4 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 EI 1931-8448 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD OCT PY 2016 VL 22 IS 7 BP 598 EP 608 DI 10.1089/mdr.2015.0259 PG 11 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA DZ4XL UT WOS:000385864800014 PM 26954009 ER PT J AU Moreno, JP Johnston, CA Hernandez, DC LeNoble, J Papaioannou, MA Foreyt, JP AF Moreno, J. P. Johnston, C. A. Hernandez, D. C. LeNoble, J. Papaioannou, M. A. Foreyt, J. P. TI Impact of parental weight status on a school-based weight management programme designed for Mexican-American children SO PEDIATRIC OBESITY LA English DT Article DE Childhood obesity; Mexican American; parents; school based ID BODY-MASS INDEX; PREDICTING OBESITY; CHILDHOOD; OVERWEIGHT; STYLE; ENVIRONMENT; ADIPOSITY; ADULTHOOD; INCREASES; YOUTH AB BackgroundWhile overweight and obese children are more likely to have overweight or obese parents, less is known about the effect of parental weight status on children's success in weight management programmes. ObjectivesThis study was a secondary data analysis of a randomized controlled trial and investigated the impact of having zero, one or two obese parents on children's success in a school-based weight management programme. MethodsSixty-one Mexican-American children participated in a 24-week school-based weight management intervention which took place in 2005-2006. Children's heights and weights were measured at baseline, 3, 6 and 12 months. Parental weight status was assessed at baseline. Repeated measures anova and ancova were conducted to compare changes in children's weight within and between groups, respectively. ResultsWithin-group comparisons revealed that the intervention led to significant decreases in standardized body mass index (zBMI) for children with zero (F=23.16, P<.001) or one obese (F=4.99, P<.05) parent. Between-group comparisons indicated that children with zero and one obese parents demonstrated greater decreases in zBMI compared to children with two obese parents at every time point. ConclusionsThe school-based weight management programme appears to be most efficacious for children with one or no obese parents compared to children with two obese parents. These results demonstrate the need to consider parental weight status when engaging in childhood weight management efforts. C1 [Moreno, J. P.; Papaioannou, M. A.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat Nutr, 1100 Bates Ave, Houston, TX 77030 USA. [Foreyt, J. P.] Baylor Coll Med, Dept Med, 1100 Bates Ave, Houston, TX 77030 USA. [Johnston, C. A.; Hernandez, D. C.] Univ Houston, Dept Hlth & Human Performance, Houston, TX USA. [LeNoble, J.] Baylor Coll Med, Dept Psychiat, 1100 Bates Ave, Houston, TX 77030 USA. [LeNoble, J.] Univ Wisconsin Hosp & Clin, Dept Orthoped & Rehabil, Madison, WI 53792 USA. RP Moreno, JP (reprint author), Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat Nutr, 1100 Bates Ave, Houston, TX 77030 USA. EM palcic@bcm.edu FU USDA/ARS [6250-51000] FX This manuscript has been supported in part by a gift from the Kellogg Company and with federal funds from the USDA/ARS 6250-51000. We are extremely grateful to the staff of YES Preparatory Academy for their dedication to improving the lives of children. JPF and CAJ conceived and designed the study and supervised the conduct of the study. JPM and CAJ conceived the concept and design of the secondary analysis of the data and conducted the statistical analyses. JPM, CAJ and DCH participated in interpretation of the findings. JPM, CAJ, DCH, JL, MAP and JPF drafted and critically reviewed the manuscript and approved the final manuscript version. We would also like to acknowledge the work of Steven Canales in helping to prepare this manuscript for publication. NR 30 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-6310 EI 2047-6302 J9 PEDIATR OBES JI Pediatr. Obes. PD OCT PY 2016 VL 11 IS 5 BP 354 EP 360 DI 10.1111/ijpo.12066 PG 7 WC Pediatrics SC Pediatrics GA DY8KM UT WOS:000385378800009 PM 26437922 ER PT J AU Kheirkhahan, M Tudor-Locke, C Axtell, R Buman, MP Fielding, RA Glynn, NW Guralnik, JM King, AC White, DK Miller, ME Siddique, J Brubaker, P Rejeski, WJ Ranshous, S Pahor, M Ranka, S Manini, TM AF Kheirkhahan, Matin Tudor-Locke, Catrine Axtell, Robert Buman, Matthew P. Fielding, Roger A. Glynn, Nancy W. Guralnik, Jack M. King, Abby C. White, Daniel K. Miller, Michael E. Siddique, Juned Brubaker, Peter Rejeski, W. Jack Ranshous, Stephen Pahor, Marco Ranka, Sanjay Manini, Todd M. TI Actigraphy features for predicting mobility disability in older adults SO PHYSIOLOGICAL MEASUREMENT LA English DT Article DE aging; disability; physical activity; sedentary; machine learning; data mining ID ARTIFICIAL NEURAL-NETWORK; LIFE-STYLE INTERVENTIONS; PHYSICAL-ACTIVITY; ENERGY-EXPENDITURE; PARKINSONS-DISEASE; ACCELEROMETER DATA; GAIT; SEDENTARY; SELECTION; RISK AB Actigraphy has attracted much attention for assessing physical activity in the past decade. Many algorithms have been developed to automate the analysis process, but none has targeted a general model to discover related features for detecting or predicting mobility function, or more specifically, mobility impairment and major mobility disability (MMD). Men (N = 357) and women (N = 778) aged 70-89 years wore a tri-axial accelerometer (Actigraph GT3X) on the right hip during free-living conditions for 8.4 +/- 3.0 d. One-second epoch data were summarized into 67 features. Several machine learning techniques were used to select features from the free-living condition to predict mobility impairment, defined as 400 m walking speed <0.80 m s(-1). Selected features were also included in a model to predict the first occurrence of MMD-defined as the loss in the ability to walk 400 m. Each method yielded a similar estimate of 400 m walking speed with a root mean square error of similar to 0.07 m s(-1) and an R-squared values ranging from 0.37-0.41. Sensitivity and specificity of identifying slow walkers was approximately 70% and 80% for all methods, respectively. The top five features, which were related to movement pace and amount (activity counts and steps), length in activity engagement (bout length), accumulation patterns of activity, and movement variability significantly improved the prediction of MMD beyond that found with common covariates (age, diseases, anthropometry, etc). This study identified a subset of actigraphy features collected in free-living conditions that are moderately accurate in identifying persons with clinically-assessed mobility impaired and significantly improve the prediction of MMD. These findings suggest that the combination of features as opposed to a specific feature is important to consider when choosing features and/or combinations of features for prediction of mobility phenotypes in older adults. C1 [Kheirkhahan, Matin; Pahor, Marco; Manini, Todd M.] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL 32611 USA. [Tudor-Locke, Catrine] Univ Massachusetts, Dept Kinesiol, Amherst, MA 01003 USA. [Axtell, Robert] Southern Connecticut State Univ, Dept Exercise Sci, New Haven, CT USA. [Buman, Matthew P.] Arizona State Univ, Sch Nutr & Hlth Promot, Tempe, AZ USA. [Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Glynn, Nancy W.] Univ Pittsburgh, Dept Epidemiol, Ctr Aging & Populat Hlth, Pittsburgh, PA 15261 USA. [Guralnik, Jack M.] Univ Maryland, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Hlth Res & Policy, Stanford, CA 94305 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [White, Daniel K.] Boston Univ, Dept Phys Therapy & Athlet Training, Boston, MA 02215 USA. [Miller, Michael E.] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Biostat Sci, Winston Salem, NC USA. [Siddique, Juned] Northwestern Univ, Dept Prevent Med, Chicago, IL 60611 USA. [Brubaker, Peter; Rejeski, W. Jack] Wake Forest Univ, Dept Hlth & Exercise Sci, Winston Salem, NC 27109 USA. [Kheirkhahan, Matin; Ranshous, Stephen; Ranka, Sanjay] Univ Florida, Dept Comp & Informat Sci & Engn, Gainesville, FL 32611 USA. RP Kheirkhahan, M (reprint author), Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL 32611 USA.; Kheirkhahan, M (reprint author), Univ Florida, Dept Comp & Informat Sci & Engn, Gainesville, FL 32611 USA. EM matin@cise.ufl.edu FU National Institutes of Health/National Institute on Aging [UO1 AG22376]; Intramural Research Program, National Institute on Aging, NIH; Claude D Pepper Older Americans Independence Centers at the University of Florida [P30 AG028740]; Wake Forest University [P30 AG21332]; Tufts University [1P30AG031679]; University of Pittsburgh [P30 AG024827]; Yale University [P30AG021342]; NIH/NCRR CTSA at Stanford University [UL1 RR025744]; Boston Rehabilitation Outcomes Center [1R24HD065688-01A1]; NIH [HL121023, AG042525, DK097364, HL075451, U54EB020404, 1R01AG049024, 1R21HD073807, 1R21OH010785]; Academic Leadership Award from the National Institute on Aging [K07AG3587]; Department of Veterans Affairs; US Department of Agriculture [58-1950-0-014]; AHA [14SFRN20740001]; National Heart, Lung and Blood Institute [3U01AG022376-05A2S] FX The Lifestyle Interventions and Independence for Elders Study is funded by a National Institutes of Health/National Institute on Aging Cooperative Agreement #UO1 AG22376 and a supplement from the National Heart, Lung and Blood Institute 3U01AG022376-05A2S, and sponsored in part by the Intramural Research Program, National Institute on Aging, NIH. The research is partially supported by the Claude D Pepper Older Americans Independence Centers at the University of Florida (P30 AG028740), Wake Forest University (P30 AG21332), Tufts University (1P30AG031679), University of Pittsburgh (P30 AG024827), and Yale University (P30AG021342) and the NIH/NCRR CTSA at Stanford University (UL1 RR025744).; Tufts University is also supported by the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1).; LIFE investigators are also partially supported by the following:; Dr Todd Manini (University of Florida) is partially supported by NIH HL121023 and AG042525.; Dr Thomas Gill (Yale University) is the recipient of an Academic Leadership Award (K07AG3587) from the National Institute on Aging.; Dr Carlos Fragoso (Spirometry Reading Center, Yale University) is the recipient of a Career Development Award from the Department of Veterans Affairs.; Dr Roger A Fielding (Tufts University) is partially supported by the US Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture.; Dr Bonnie Spring is partially supported by NIH DK097364, HL075451, U54EB020404, and AHA 14SFRN20740001; Dr Catrine Tudor-Locke is partially supported by NIH 1R01AG049024, 1R21HD073807, and 1R21OH010785. NR 50 TC 1 Z9 1 U1 7 U2 7 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0967-3334 EI 1361-6579 J9 PHYSIOL MEAS JI Physiol. Meas. PD OCT PY 2016 VL 37 IS 10 BP 1813 EP 1833 DI 10.1088/0967-3334/37/10/1813 PG 21 WC Biophysics; Engineering, Biomedical; Physiology SC Biophysics; Engineering; Physiology GA DY9ZW UT WOS:000385497200013 PM 27653966 ER PT J AU Li, JY Zhu, LZ Hull, JJ Liang, SJ Daniell, H Jin, SX Zhang, XL AF Li, Jianying Zhu, Lizhen Hull, J. Joe Liang, Sijia Daniell, Henry Jin, Shuangxia Zhang, Xianlong TI Transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect Bemisia tabaci (whitefly) SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE cotton; whitefly; RNA-Seq; DEGs; co-expression; transcription factor ID PLANT DEFENSE; VERTICILLIUM-DAHLIAE; NICOTIANA-ATTENUATA; EXPRESSION ANALYSIS; MOLECULAR-PATTERNS; SPODOPTERA-LITURA; NETWORK ANALYSIS; ARABIDOPSIS; GENES; HERBIVORES AB The whitefly (Bemisia tabaci) causes tremendous damage to cotton production worldwide. However, very limited information is available about how plants perceive and defend themselves from this destructive pest. In this study, the transcriptomic differences between two cotton cultivars that exhibit either strong resistance (HR) or sensitivity (ZS) to whitefly were compared at different time points (0, 12, 24 and 48h after infection) using RNA-Seq. Approximately one billion paired-end reads were obtained by Illumina sequencing technology. Gene ontology and KEGG pathway analysis indicated that the cotton transcriptional response to whitefly infestation involves genes encoding protein kinases, transcription factors, metabolite synthesis, and phytohormone signalling. Furthermore, a weighted gene co-expression network constructed from RNA-Seq datasets showed that WRKY40 and copper transport protein are hub genes that may regulate cotton defenses to whitefly infestation. Silencing GhMPK3 by virus-induced gene silencing (VIGS) resulted in suppression of the MPK-WRKY-JA and ET pathways and lead to enhanced whitefly susceptibility, suggesting that the candidate insect resistant genes identified in this RNA-Seq analysis are credible and offer significant utility. Taken together, this study provides comprehensive insights into the cotton defense system to whitefly infestation and has identified several candidate genes for control of phloem-feeding pests. C1 [Li, Jianying; Zhu, Lizhen; Liang, Sijia; Jin, Shuangxia; Zhang, Xianlong] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China. [Hull, J. Joe] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Daniell, Henry] Univ Penn, Sch Dent Med, Dept Biochem, Philadelphia, PA 19104 USA. RP Jin, SX (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China. EM jsx@mail.hzau.edu.cn FU National Natural Science Foundation of China [C130406]; Ministry of Agriculture [CRAS-18-09]; Fundamental Research Funds for the Central Universities [2013PY064] FX We thank Mr. Qinghua Zhang from the National Key Laboratory of Crop Genetic Improvement at Huazhong Agricultural University for help in RNA library construction and sequencing. This work was supported by grants from National Natural Science Foundation of China (C130406), Ministry of Agriculture grant (No. CRAS-18-09), and Fundamental Research Funds for the Central Universities (2013PY064). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 81 TC 2 Z9 3 U1 26 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD OCT PY 2016 VL 14 IS 10 BP 1956 EP 1975 DI 10.1111/pbi.12554 PG 20 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DW4PI UT WOS:000383625100002 PM 26923339 ER PT J AU Blackman, CJ Pfautsch, S Choat, B Delzon, S Gleason, SM Duursma, RA AF Blackman, Chris J. Pfautsch, Sebastian Choat, Brendan Delzon, Sylvain Gleason, Sean M. Duursma, Remko A. TI Toward an index of desiccation time to tree mortality under drought SO PLANT CELL AND ENVIRONMENT LA English DT Article ID HYDRAULIC FAILURE; WATER-STRESS; VEGETATION MORTALITY; WOODY ANGIOSPERMS; RADIAL TRANSFER; SUMMER DROUGHT; XYLEM; CAVITATION; VULNERABILITY; CAPACITANCE AB Research in plant hydraulics has provided important insights into plant responses to drought and species absolute drought tolerance. However, our ability to predict when plants will die from hydraulic failure under extreme drought is limited by a lack of knowledge with regards to the dynamics of plant desiccation following stomatal closure. Thus, we develop a simple hydraulics model based on branch-level traits that incorporates key aspects of allometry, rates of water loss and resistance to embolism thresholds in order to define species differences in the time it takes plants to desiccate from stomatal closure to lethal levels of drought stress. C1 [Blackman, Chris J.; Pfautsch, Sebastian; Choat, Brendan; Duursma, Remko A.] Univ Western Sydney, Hawkesbury Inst Environm, Locked Bag 1797, Penrith, NSW 2751, Australia. [Delzon, Sylvain] Univ Bordeaux, INRA, BIOGECO, F-33610 Cestas, France. [Gleason, Sean M.] USDA ARS, Water Management Res, 2150 Ctr Ave,Build D,Suite 320, Ft Collins, CO 80526 USA. RP Blackman, CJ (reprint author), Univ Western Sydney, Hawkesbury Inst Environm, Locked Bag 1797, Penrith, NSW 2751, Australia. EM c.blackman@westernsydney.edu.au RI Pfautsch, Sebastian/A-2008-2016; Pfautsch, Sebastian/J-8676-2012 OI Pfautsch, Sebastian/0000-0002-4390-4195; Pfautsch, Sebastian/0000-0002-4390-4195 NR 45 TC 0 Z9 0 U1 20 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD OCT PY 2016 VL 39 IS 10 BP 2342 EP 2345 DI 10.1111/pce.12758 PG 4 WC Plant Sciences SC Plant Sciences GA DZ4RE UT WOS:000385846100018 PM 27093688 ER PT J AU Des Marais, DL Razzaque, S Hernandez, KM Garvin, DF Juenger, TE AF Des Marais, David L. Razzaque, Samsad Hernandez, Kyle M. Garvin, David F. Juenger, Thomas E. TI Quantitative trait loci associated with natural diversity in water-use efficiency and response to soil drying in Brachypodium distachyon SO PLANT SCIENCE LA English DT Article DE Water Use Efficiency; GxE; QTL mapping; Epistasis; Abiotic stress; Brachypodium distachyon ID CARBON-ISOTOPE DISCRIMINATION; ARABIDOPSIS-THALIANA; DROUGHT ADAPTATION; GENETIC-VARIATION; INBRED LINES; TRANSPIRATION EFFICIENCY; ECOPHYSIOLOGICAL TRAITS; BREAD WHEAT; PLANT; QTL AB All plants must optimize their growth with finite resources. Water use efficiency (WUE) measures the relationship between biomass acquisition and transpired water. In the present study, we performed two experiments to understand the genetic basis of WUE and other parameters of plant-water interaction under control and water-limited conditions. Our study used two inbred natural accessions of Brachy-podium distachyon, a model grass species with close phylogenetic affinity to temperate forage and cereal crops. First, we identify the soil water content which causes a reduction in leaf relative water content and an increase in WUE. Second, we present results from a large phenotyping experiment utilizing a recombinant inbred line mapping population derived from these same two natural accessions. We identify QTLs associated with environmentally-insensitive genetic variation in WUE, including a pair of epistatically interacting loci. We also identify QTLs associated with constitutive differences in biomass and a QTL describing an environmentally-sensitive difference in leaf carbon content. Finally, we present a new linkage map for this mapping population based on new SNP markers as well as updated genomic positions for previously described markers. Our studies provide an initial characterization of plant-water relations in B. distachyon and identify candidate genomic regions involved in WUE. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Des Marais, David L.; Razzaque, Samsad; Hernandez, Kyle M.; Juenger, Thomas E.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [Des Marais, David L.; Razzaque, Samsad; Hernandez, Kyle M.; Juenger, Thomas E.] Univ Texas Austin, Inst Cell & Mol Biol, Austin, TX 78712 USA. [Garvin, David F.] ARS, USDA, Plant Sci Res Unit, St Paul, MN USA. [Des Marais, David L.] Harvard Univ, Arnold Arboretum, Cambridge, MA 02138 USA. [Des Marais, David L.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Hernandez, Kyle M.] Univ Chicago, Ctr Res Informat, Chicago, IL 60637 USA. RP Des Marais, DL (reprint author), 1300 Ctr St, Roslindale, MA 02131 USA.; Des Marais, DL (reprint author), Harvard Univ, Arnold Arboretum, Cambridge, MA 02138 USA.; Des Marais, DL (reprint author), Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. EM desmarais@fas.harvard.edu FU USDA NIFA award [2011-67012-30663]; NSF Postdoctoral Research Fellowship [DBI-1103668]; USDA CRIS Project [5062-21000-030-00D]; NSF Plant Genome Research [IOS-0922457] FX Colin Purmal, Kathleen Burns, Emeline Sukamtoh, Omar Gonzales, and Erin Atkinson assisted with RIL phenotyping. Tierney Logan extracted DNA and prepared the SOLD libraries for sequencing. Comments from E. Blumwald and two anonymous reviewers greatly improved the manuscript. This work was supported by a USDA NIFA award (2011-67012-30663) to D.L.D., an NSF Postdoctoral Research Fellowship (DBI-1103668) to K.M.H, USDA CRIS Project Award (5062-21000-030-00D) to D.F.G., and an NSF Plant Genome Research Program award (IOS-0922457) to T.E.J. NR 74 TC 2 Z9 2 U1 18 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD OCT PY 2016 VL 251 SI SI BP 2 EP 11 DI 10.1016/j.plantsci.2016.03.010 PG 10 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DY7SH UT WOS:000385329100002 PM 27593458 ER PT J AU Costa, MCD Farrant, JM Oliver, MJ Ligterink, W Buitink, J Hilhorst, HMW AF Costa, Maria Cecilia D. Farrant, Jill M. Oliver, Melvin J. Ligterink, Wilco Buitink, Julia Hilhorst, Henk M. W. TI Key genes involved in desiccation tolerance and dormancy across life forms SO PLANT SCIENCE LA English DT Article DE AB13; Arabidopsis thaliana; Conserved genes; Desiccation tolerance; Dormancy ID EMBRYOGENESIS-ABUNDANT PROTEIN; ARABIDOPSIS-THALIANA; RESURRECTION PLANTS; SEEDS; EXPRESSION; EVOLUTION; MECHANISM; REESTABLISHMENT; DEHYDRATION; CONSTRAINTS AB Desiccation tolerance (DT, the ability of certain organisms to survive severe dehydration) was a key trait in the evolution of life in terrestrial environments. Likely, the development of desiccation-tolerant life forms was accompanied by the acquisition of dormancy or a dormancy-like stage as a second powerful adaptation to cope with variations in the terrestrial environment. These naturally stress tolerant life forms may be a good source of genetic information to generate stress tolerant crops to face a future with predicted higher occurrence of drought. By mining for key genes and mechanisms related to DT and dormancy conserved across different species and life forms, unique candidate key genes may be identified. Here we identify several of these putative key genes, shared among multiple organisms, encoding for proteins involved in protection, growth and energy metabolism. Mutating a selection of these genes in the model plant Arabidopsis thaliana resulted in clear DT-, dormancy- and other seed-associated phenotypes, showing the efficiency and power of our approach and paves the way for the development of drought stress tolerant crops. Our analysis supports a co-evolution of DT and dormancy by shared mechanisms that favour survival and adaptation to ever-changing environments with strong seasonal fluctuations. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Costa, Maria Cecilia D.; Ligterink, Wilco; Hilhorst, Henk M. W.] Wageningen Univ, Lab Plant Physiol, Wageningen Seed Lab, NL-6708 PB Wageningen, Netherlands. [Farrant, Jill M.] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa. [Oliver, Melvin J.] Univ Missouri, USDA, ARS, MWA,PGRU, 205 Curtis Hall, Columbia, MO 65211 USA. [Buitink, Julia] INRA, UMR 1345, Inst Rech Hort & Semences, SFR Qual & Sante Vegetal 4207, F-49045 Angers, France. RP Costa, MCD (reprint author), Wageningen Univ, Lab Plant Physiol, Wageningen Seed Lab, NL-6708 PB Wageningen, Netherlands. EM macecilia@ymail.com RI Hilhorst, Henk/K-1658-2013; Ligterink, Wilco/C-8103-2009; Costa, Maria Cecilia/C-8980-2016 OI Ligterink, Wilco/0000-0002-0228-169X; Costa, Maria Cecilia/0000-0003-0083-0904 FU 'Conselho Nacional de Desenvolvimento Cientifico e Tecnologico' (CNPq, Brazil) FX This work was supported by the 'Conselho Nacional de Desenvolvimento Cientifico e Tecnologico' (CNPq, Brazil). NR 55 TC 1 Z9 1 U1 18 U2 19 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD OCT PY 2016 VL 251 SI SI BP 162 EP 168 DI 10.1016/j.plantsci.2016.02.001 PG 7 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DY7SH UT WOS:000385329100018 PM 27593474 ER PT J AU Crook, EK O'Brien, DJ Howell, SB Storey, BE Whitley, NC Burke, JM Kaplan, RM AF Crook, E. K. O'Brien, D. J. Howell, S. B. Storey, B. E. Whitley, N. C. Burke, J. M. Kaplan, R. M. TI Prevalence of anthelmintic resistance on sheep and goat farms in the mid-Atlantic region and comparison of in vivo and in vitro detection methods SO SMALL RUMINANT RESEARCH LA English DT Article DE Anthelmintic resistance; FECRT; Gastrointestinal nematode; Goats; Larval developmental assay; Sheep ID LARVAL DEVELOPMENT ASSAY; GASTROINTESTINAL NEMATODES; VETERINARY IMPORTANCE; UNITED-STATES; MONEPANTEL; EFFICACY AB Despite strong economic opportunities and incentives for small ruminant production, their health and productivity are often severely affected by parasitic disease. To combat these effects, most farms administer anthelmintics to their animals at frequent intervals, and without consideration to principles of sustainable integrated parasite management (SIPM). This has led to growing problems caused by the development of drug-resistant populations of gastrointestinal nematodes (GIN) in much of the world, particularly in Haemonchus contortus. The objectives of this research were to characterize levels of anthelmintic resistance on small ruminant farms located in the mid-Atlantic US and to compare the fecal egg count reduction test (FECRT) and larval development assay (LDA) for detecting resistance. To achieve these objectives, the DrenchRite (R) LDA was used to evaluate resistance status to benzimidazoles, ivermectin, moxidectin, and levamisole on 20 goat and 14 sheep farms in the Mid-Atlantic US over a 2-year period. A FECRT was also conducted on 14 of the same farms and on 2 additional farms in which the LDA was not completed. For the LDA and coprocultures, fecal samples were collected rectally from a minimum of 10 individual animals, pooled, and express-mailed to the University of Georgia for analysis. For the FECRT, albendazole, ivermectin, moxidectin, and/or levamisole were tested on each farm. Animals were allocated randomly based on FAMACHA (c) scores to 2-5 treatment groups, which included an untreated control group. The number of treatment groups on a farm depended on the number of qualified animals present. Haemonchus contortus was the most common parasite recovered from fecal cultures; the mean level across all farms was 79%. Results of the LDA indicated resistance to benzimidazoles, ivermectin, moxidectin, and levamisole on 100%, 82%, 47%, and 24% of farms, respectively. Multi-drug resistance to all 3 drug classes was detected for H. contortus on 18% of farms (1 sheep and 5 goat farms). Of the 16 farms tested by FECRT, resistance to albendazole was present on 8/10 farms, to ivermectin on 4/4 farms, to moxidectin on 7/9 farms and to levamisole on 2/5 farms tested. Results obtained from the FECRT and the LDA (p = 0.51) were similar. The prevalence of resistance found in this study in the mid Atlantic region of the US is very similar to that reported in an earlier survey of resistance performed in the Southern US, demonstrating that anthelmintic resistance in GIN is a serious problem on small ruminant farms throughout the Eastern US. (C) 2016 Elsevier B.V. All rights reserved. C1 [Crook, E. K.; O'Brien, D. J.] Delaware State Univ, Dept Agr, 1200 N Dupont Hwy, Dover, DE 19901 USA. [Howell, S. B.; Storey, B. E.; Kaplan, R. M.] Univ Georgia, Dept Infect Dis, Coll Vet Med, Athens, GA 30602 USA. [Whitley, N. C.] Ft Valley State Univ, Cooperat Extens Program, Ft Valley, GA 31030 USA. [Burke, J. M.] ARS, Dale Bumpers Small Farms Res Ctr, USDA, Booneville, AR 72927 USA. [O'Brien, D. J.] Virginia State Univ, Cooperat Extens, 1 Hayden Dr, Petersburg, VA 23806 USA. [Crook, E. K.] Shore Large Anim Vet Serv LLC, POB 223, Centreville, MD 21617 USA. RP O'Brien, DJ (reprint author), Delaware State Univ, Dept Agr, 1200 N Dupont Hwy, Dover, DE 19901 USA.; O'Brien, DJ (reprint author), Virginia State Univ, Cooperat Extens, 1 Hayden Dr, Petersburg, VA 23806 USA. EM dobrien@vsu.edu FU USDA-CSREES Capacity Building Grant [DELX-2007-03467] FX Funding was supported by the USDA-CSREES Capacity Building Grant (DELX-2007-03467). The authors thank Susan Schoenian, Sheep and Goat Specialist in Western Maryland for promoting the project and assisting in the identification of participating farms as well as Delaware State University graduate student, Kwame Matthews, and farm technician, Lee Dulin, for assisting with this research project. NR 23 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-4488 EI 1879-0941 J9 SMALL RUMINANT RES JI Small Ruminant Res. PD OCT PY 2016 VL 143 BP 89 EP 96 DI 10.1016/j.smallrumres.2016.09.006 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DZ1MQ UT WOS:000385602900013 ER PT J AU Huang, YJS Ayers, VB Lyons, AC Unlu, I Alto, BW Cohnstaedt, LW Higgs, S Vanlandingham, DL AF Huang, Yan-Jang S. Ayers, Victoria B. Lyons, Amy C. Unlu, Isik Alto, Barry W. Cohnstaedt, Lee W. Higgs, Stephen Vanlandingham, Dana L. TI Culex Species Mosquitoes and Zika Virus SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Culex species mosquitoes; refractoriness; Zika virus ID AEDES-ALBOPICTUS; ISOLATIONS; AEGYPTI; THREAT; IMPACT AB Recent reports of Zika virus (ZIKV) isolates from Culex species mosquitoes have resulted in concern regarding a lack of knowledge on the number of competent vector species for ZIKV transmission in the new world. Although observations in the field have demonstrated that ZIKV isolation can be made from Culex species mosquitoes, the detection of ZIKV in these mosquitoes is not proof of their involvement in a ZIKV transmission cycle. Detection may be due to recent feeding on a viremic vertebrate, and is not indicative of replication in the mosquito. In this study, susceptibility of recently colonized Culex species mosquitoes was investigated. The results showed a high degree of refractoriness among members of Culex pipiens complex to ZIKV even when exposed to high-titer bloodmeals. Our finding suggests that the likelihood of Culex species mosquitoes serving as secondary vectors for ZIKV is very low, therefore vector control strategies for ZIKV should remain focused on Aedes species mosquitoes. Our demonstration that Culex quinquefasciatus from Vero Beach, FL, is refractory to infection with ZIKV is especially important and timely. Based on our data, we would conclude that the autochthonous cases of Zika in Florida are not due to transmission by C. quinquefasciatus, and so control efforts should focus on other species, logically Aedes aegypti and Aedes albopictus. C1 [Huang, Yan-Jang S.; Ayers, Victoria B.; Lyons, Amy C.; Higgs, Stephen; Vanlandingham, Dana L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Huang, Yan-Jang S.; Ayers, Victoria B.; Lyons, Amy C.; Higgs, Stephen; Vanlandingham, Dana L.] Kansas State Univ, Biosecur Res Inst, Manhattan, KS 66506 USA. [Unlu, Isik] Rutgers State Univ, Ctr Vector Biol, New Brunswick, NJ USA. [Unlu, Isik] Mercer Cty Mosquito Control, Trenton, NJ USA. [Alto, Barry W.] Univ Florida, Florida Med Entomol Lab, Vero Beach, FL USA. [Cohnstaedt, Lee W.] ARS, Arthropod Borne Anim Dis Res Unit, USDA, Manhattan, KS USA. RP Vanlandingham, DL (reprint author), Kansas State Univ, Dept Diagnost Med Pathobiol, K-221 Mosier Hall, Manhattan, KS 66506 USA. EM dlvanlan@vet.ksu.edu FU Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University FX The authors would like to thank Christopher Clarkston, Ryan Dajczak, Susan Hettenbach, Brittany Hilfiker, Nick Indelicato, Sara Ortiz, Sydney Rathjen, and Kathryn Ryan for their technical assistance. The research is supported by the startup fund of the Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University (PI: D.L.V.). The funding body has no role in the design of the study, collection, analysis, and interpretation of data or writing of the article. NR 22 TC 5 Z9 5 U1 114 U2 114 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD OCT PY 2016 VL 16 IS 10 BP 673 EP 676 DI 10.1089/vbz.2016.2058 PG 4 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DY8QO UT WOS:000385395100008 PM 27556838 ER PT J AU Faust, DR Kroger, R Miranda, LE Rush, SA AF Faust, Derek R. Kroger, Robert Miranda, Leandro E. Rush, Scott A. TI Nitrate Removal from Agricultural Drainage Ditch Sediments with Amendments of Organic Carbon: Potential for an Innovative Best Management Practice SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Agriculture; Amendments; Microcosms; Nitrate; Nutrients; Organic carbon ID LOW-GRADE WEIRS; MISSISSIPPI RIVER-BASIN; HYDROLOGICAL VARIABILITY; PHOSPHORUS MANAGEMENT; NUTRIENT REMOVAL; DENITRIFICATION; REDUCTION; ECOSYSTEM; STREAM; AQUACULTURE AB Agricultural fertilizer applications have resulted in loading of nutrients to agricultural drainage ditches in the Lower Mississippi Alluvial Valley. The purpose of this study was to determine effects of dissolved organic carbon (DOC) and particulate organic carbon (POC) amendments on nitrate-nitrogen (NO3--N) removal from overlying water, pore water, and sediment of an agricultural drainage ditch. Two experiments were conducted. In experiment 1, control (i.e., no amendment), DOC, and POC treatments were applied in laboratory microcosms for time intervals of 3, 7, 14, and 28 days. In experiment 2, control, DOC, and POC treatments were applied in microcosms at C/N ratios of 5:1, 10:1, 15:1, and 20:1. There were statistically significant effects of organic carbon amendments in experiment 1 (F-2,F-71 = 27.1, P < 0.001) and experiment 2 (F-2,F-53 = 39.1, P < 0.001), time (F-1,F-71 = 14.5, P < 0.001) in experiment 1, and C/N ratio (F-1,F-53 = 36.5, P < 0.001) in experiment 2. NO3--N removal varied from 60 to 100 % in overlying water among all treatments. The lowest NO3--N removals in experiment 1 were observed in the control at 14 and 28 days, which were significantly less than in DOC and POC 14- and 28-day treatments. In experiment 2, significantly less NO3--N was removed in overlying water of the control compared to DOC and POC treatments at all C/N ratios. Amendments of DOC and POC made to drainage ditch sediment: (1) increased NO3--N removal, especially over longer time intervals (14 to 28 days); (2) increased NO3--N removal, regardless of C/N ratio; and (3) NO3--N removal was best at a 5:1 C/N ratio. This study provides support for continued investigation on the use of organic carbon amendments as a best management practice for NO3--N removal in agricultural drainage ditches. C1 [Faust, Derek R.; Rush, Scott A.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Box 9690, Mississippi State, MS 39762 USA. [Kroger, Robert] Covington Civil & Environm LLC, 2510 14th St,Ste 1010, Gulfport, MS 39501 USA. [Miranda, Leandro E.] US Geol Survey, Mississippi Cooperat Fish andWildlife Res Unit, Box 9691, Mississippi State, MS 39762 USA. [Faust, Derek R.] ARS, Northern Great Plains Res Lab, USDA, POB 459, Mandan, ND 58554 USA. RP Faust, DR (reprint author), Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Box 9690, Mississippi State, MS 39762 USA.; Faust, DR (reprint author), ARS, Northern Great Plains Res Lab, USDA, POB 459, Mandan, ND 58554 USA. EM derek.faust@ars.usda.gov FU Forest and Wildlife Research Center of Mississippi State University; Mississippi Agricultural and Forestry Experiment Station of Mississippi State University FX We thank the Forest and Wildlife Research Center and Mississippi Agricultural and Forestry Experiment Station of Mississippi State University for financial support. We also extend gratitude to past and present members of the Water Quality Laboratory at Mississippi State University for their valuable contributions to this project. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 47 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD OCT PY 2016 VL 227 IS 10 AR 378 DI 10.1007/s11270-016-3075-9 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA DY8YT UT WOS:000385419200021 ER PT J AU Jiang, XL Kang, SZ Li, FS Du, TS Tong, L Comas, L AF Jiang, Xuelian Kang, Shaozhong Li, Fusheng Du, Taisheng Tong, Ling Comas, Louise TI Evapotranspiration partitioning and variation of sap flow in female and male parents of maize for hybrid seed production in arid region SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Evapotranspiration; Transpiration; Soil evaporation; Compensatory effect; Meteorological variable; Soil moisture ID NORTHWEST CHINA; CROP COEFFICIENT; MICROLYSIMETRY TECHNIQUES; ENVIRONMENTAL-FACTORS; COMPENSATORY GROWTH; FIELD CONDITIONS; EDDY COVARIANCE; TRANSPIRATION; WATER; BALANCE AB Understanding the variation of sap flow in female and male parents of maize for hybrid seed production and evapotranspiration (ET) partitioning is useful in accurately determining water use of the female and male parents and improving irrigation management of maize for hybrid seed production. Sap flow rate, ET, soil evaporation (E), meteorological variable, soil water content (SWC) and morphological parameters in female and male parents were measured during April to September in both 2013 and 2014 in the arid region of northwest China. Sap flow rates in female and male parents and E were respectively monitored using heat-balance technique and micro-lysimeter. We found that the variations of sap flow rates in female and male parents were similar during the vegetative growth stage, but the female parents had higher sap flow rate per plant than the male parents after the detasseling, with the maximum difference of daily sap flow rate per plant of 0.28-0.33 L d(-1). Hourly sap flow rates in female and male parents were highly correlated with net radiation, while daily sap flow rates in female and male parents were highly correlated with net radiation, SWC, leaf area index, but not correlated with crop height. Daily E increased exponentially with SWC. Moreover, daily sap flow rates in the male parents were positively correlated with stem diameter. The total ET was 363.31-384.15 mm over the whole growth stage of maize, and the transpiration (T) of female parents per ground area accounted for 63-80% of the total ET, while the T of male parents per ground area accounted for 4-14% of the total ET, primarily due to different planting quantities between the female and male parents. And the soil evaporation was 47.77-62.21 mm over the whole growth stage, accounting for 13-16% of the total ET. Our results can provide scientific basis for accurate determination of water use of female and male plants and developing precise irrigation scheduling of maize for hybrid seed production in the arid region. (C) 2016 Published by Elsevier B.V. C1 [Jiang, Xuelian; Kang, Shaozhong; Du, Taisheng; Tong, Ling] China Agr Univ, Ctr Agr Water Res China, Beijing 100083, Peoples R China. [Li, Fusheng] Guangxi Univ, Coll Agr, Nanning 530005, Guangxi, Peoples R China. [Comas, Louise] USDA ARS, Water Management Res Unit, Ft Collins, CO 80522 USA. RP Tong, L (reprint author), China Agr Univ, Ctr Agr Water Res China, Beijing 100083, Peoples R China. EM tongling2001@cau.edu.cn RI kang, Shaozhong/C-5434-2011; Li, Fusheng/E-4914-2011 OI Li, Fusheng/0000-0003-4189-3354 FU National Natural Science Foundation of China [91425302, 51321001, 51379208]; Discipline Innovative Engineering Plan (111 Program) [B14002] FX We greatly appreciate the research grants from the National Natural Science Foundation of China (91425302, 51321001, 51379208), and The Discipline Innovative Engineering Plan (111 Program, B14002). NR 49 TC 1 Z9 1 U1 14 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD OCT PY 2016 VL 176 BP 132 EP 141 DI 10.1016/j.agwat.2016.05.022 PG 10 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DY1OY UT WOS:000384865500013 ER PT J AU Matthan, NR Ausman, LM Meng, HC Tighiouart, H Lichtenstein, AH AF Matthan, Nirupa R. Ausman, Lynne M. Meng, Huicui Tighiouart, Hocine Lichtenstein, Alice H. TI Estimating the reliability of glycemic index values and potential sources of methodological and biological variability SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE glycemic index; variability; healthy volunteers; insulin index; glycated hemoglobin ID RISK-FACTORS; INSULINEMIC RESPONSES; PROSPECTIVE COHORT; LOAD; FOODS; CARBOHYDRATE; DISEASE; METAANALYSIS; BREAKFAST; SIMILARITY AB Background: The utility of glycemic index (GI) values for chronic disease risk management remains controversial. Although absolute GI value determinations for individual foods have been shown to vary significantly in individuals with diabetes, there is a dearth of data on the reliability of GI value determinations and potential sources of variability among healthy adults. Objective: We examined the intra- and inter-individual variability in glycemic response to a single food challenge and methodologic and biological factors that potentially mediate this response. Design: The GI value for white bread was determined by using standardized methodology in 63 volunteers free from chronic disease and recruited to differ by sex, age (18-85 y), and body mass index [BMI (in kg/m(2)): 20-35]. Volunteers randomly underwent 3 sets of food challenges involving glucose (reference) and white bread (test food), both providing 50 g available carbohydrates. Serum glucose and insulin were monitored for 5 h postingestion, and GI values were calculated by using different area under the curve (AUC) methods. Biochemical variables were measured by using standard assays and body composition by dual-energy X-ray absorptiometry. Results: The mean +/- SD GI value for white bread was 62 +/- 15 when calculated by using the recommended method. Mean intra- and interindividual CVs were 20% and 25%, respectively. Increasing sample size, replication of reference and test foods, and length of blood sampling, as well as AUC calculation method, did not improve the CVs. Among the biological factors assessed, insulin index and glycated hemoglobin values explained 15% and 16% of the variability in mean GI value for white bread, respectively. Conclusions: These data indicate that there is substantial variability in individual responses to GI value determinations, demonstrating that it is unlikely to be a good approach to guiding food choices. Additionally, even in healthy individuals, glycemic status significantly contributes to the variability in GI value estimates. C1 [Matthan, Nirupa R.; Ausman, Lynne M.; Meng, Huicui; Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tighiouart, Hocine] Tufts Clin & Translat Sci Inst, Tufts Med Ctr, Boston, MA USA. RP Matthan, NR (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM nirupa.matthan@tufts.edu RI Meng, Huicui/E-9497-2017 OI Meng, Huicui/0000-0002-6090-8622 FU National Institute of Diabetes and Digestive and Kidney Diseases/NIH [DK 0733221]; National Center for Advancing Translational Sciences, NIH [UL1TR001064]; USDA [58-1950-4-401] FX This work was supported by grants DK 0733221 from the National Institute of Diabetes and Digestive and Kidney Diseases/NIH, UL1TR001064 from the National Center for Advancing Translational Sciences, NIH, and the USDA under agreement no. 58-1950-4-401. NR 46 TC 4 Z9 4 U1 10 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2016 VL 104 IS 4 BP 1004 EP 1013 DI 10.3945/ajcn.116.137208 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DY1SO UT WOS:000384874900011 PM 27604773 ER PT J AU Switkowski, KM Jacques, PF Must, A Kleinman, KP Gillman, MW Oken, E AF Switkowski, Karen M. Jacques, Paul F. Must, Aviva Kleinman, Ken P. Gillman, Matthew W. Oken, Emily TI Maternal protein intake during pregnancy and linear growth in the offspring SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE linear growth; pregnancy; protein; cohort; maternal diet; programming ID FOOD FREQUENCY QUESTIONNAIRE; IGF-I AXIS; DIETARY-PROTEIN; BIRTH-WEIGHT; FETAL-GROWTH; MULTIPLE IMPUTATION; PROSPECTIVE COHORT; MILK CONSUMPTION; GESTATIONAL-AGE; INFANT AB Background: Observational and experimental evidence demonstrates that protein intake in infancy programs linear growth. To our knowledge, few studies have examined prenatal maternal protein intake. Objective: Our objective was to examine associations of maternal protein intake during pregnancy with offspring linear growth. Design: We analyzed data from 1961 mother-child pairs in Project Viva. We assessed first- and second-trimester diet with the use of food frequency questionnaires and analyzed protein intake as grams per kilogram prepregnancy weight per day. We used research measures of offspring length at birth and in infancy (similar to 6 mo), early childhood (similar to 3 y), and midchildhood (similar to 7 y), as well as clinical growth measures obtained from after birth through midchildhood. We calculated sex specific birth length z scores for gestational age with the use of international reference data. We used mixed models with repeated length measures to predict individual length gain velocities for birth to <6 mo and 6 mo to 7 y of age, then used these velocities as outcomes in adjusted linear regression models with maternal protein intake as the main predictor. Results: Mean (range) second-trimester protein intake was 1.4 g . kg(-1) . d(-1) (0.3-3.1 g . kg(-1) . d(-1)). After adjusting for maternal sociodemographics, gestational weight gain, maternal and paternal height, and (for postdelivery outcomes) child sex, gestational age, and breastfeeding duration, each 1-SD (0.36 g . kg(-1) . d(-1)) increment in second-trimester protein intake corresponded to a-0.10 (95% CI:-0.18,-0.03) change in birth length z score, a-0.03 cm/mo (95% CI:-0.05,-0.01 cm/mo) change in slope of length growth from birth to <6mo, and a-0.09 cm/y (95% CI:-0.14,-0.05 cm/y) change in slope of length growth from 6 mo to midchildhood. Results were similar for first-trimester intake. Conclusions: In a population with relatively high protein intake during pregnancy, higher protein intake was associated with shorter offspring birth length and slower linear growth into midchildhood. Results suggest that higher protein intake during pregnancy does not increase fetal and child growth and may even reduce early length growth. Project Viva was registered at clinicaltrials.gov as NCT02820402. C1 [Switkowski, Karen M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Jacques, Paul F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Must, Aviva] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. [Switkowski, Karen M.; Kleinman, Ken P.; Gillman, Matthew W.; Oken, Emily] Harvard Med Sch, Dept Populat Med, Obes Prevent Program, Boston, MA USA. [Switkowski, Karen M.; Kleinman, Ken P.; Gillman, Matthew W.; Oken, Emily] Harvard Pilgrim Hlth Care Inst, Boston, MA USA. RP Switkowski, KM (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA.; Switkowski, KM (reprint author), Harvard Med Sch, Dept Populat Med, Obes Prevent Program, Boston, MA USA.; Switkowski, KM (reprint author), Harvard Pilgrim Hlth Care Inst, Boston, MA USA. EM karen_switkowski@harvardpilgrim.org FU NIH [R37 HD034568-09]; [K24 HD069408]; [P30 DK092924] FX Supported by the NIH (grant R37 HD034568-09). EO was supported by grants K24 HD069408 and P30 DK092924. NR 49 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2016 VL 104 IS 4 BP 1128 EP 1136 DI 10.3945/ajcn.115.128421 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DY1SO UT WOS:000384874900024 PM 27581477 ER PT J AU Garaulet, M Vera, B Bonnet-Rubio, G Gomez-Abellan, P Lee, YC Ordovas, JM AF Garaulet, Marta Vera, Beatriz Bonnet-Rubio, Gemma Gomez-Abellan, Purificacion Lee, Yu-Chi Ordovas, Jose M. TI Lunch eating predicts weight-loss effectiveness in carriers of the common allele at PERILIPIN1: the ONTIME (Obesity, Nutrigenetics, Timing, Mediterranean) study SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE late eating; Perilipin; food timing; weight loss; obesity; personalized nutrition ID GENETIC RISK SCORE; BODY-MASS INDEX; METABOLIC SYNDROME; BEHAVIORAL TREATMENT; FAT INTAKE; PLIN; DIET; POLYMORPHISMS; ASSOCIATION; POPULATION AB Background: We propose that eating lunch late impairs the mobilization of fat from adipose tissue, particularly in carriers of PERILIPIN1 (PLIN1) variants. Objective: The aim was to test the hypothesis that PLINI, a circadian lipid-stabilizing protein in the adipocyte, interacts with the timing of food intake to affect weight loss. Design: A total of 1287 overweight and obese subjects [229 men and 1058 women; mean +/- SD body mass index (in kg/m(2)): 31 +/- 5] who attended outpatient obesity clinics were enrolled in the ONTIME (Obesity, Nutrigenetics, Timing, Mediterranean) study. Timing of food intake was estimated with a validated questionnaire. Anthropometric variables and PLINI genotypes were analyzed, including 6209T>C (rs2289487), 11482G>A (rs894160), 13041A>G (rs2304795), and 14995A>T (rs1052700). The main outcomes were effectiveness of the program and weight-loss progression during 28 wk of treatment. Results: The PLINI locus was associated with variability in response to a weight-loss program. Specifically, carrying the minor C allele at the PLIN1 6209T>C was associated with better weight-loss response (P = 0.035). The probability of being a better responder [percentage of weight loss >= 7.5% (median)] was 33% higher among C than among TT carriers (OR: 1.32; 95% CI: 1.05, 1.67; P = 0.017). We found an interaction of PUN] X food timing between the 14995A>T variant and timing of lunch eating for total weight loss (P = 0.035). Among AA carriers, eating late was associated with less weight loss (P < 0.001), whereas time of eating did not influence weight loss among TT carriers (P = 0.326). Conclusions: Variability at the PLINI locus is associated with variability in weight loss. Moreover, eating late is related to lower weight-loss effectiveness among carriers of the AA genotype at the PLIN1 14995A>T variant. These results contribute to our ability to implement more precise and successful obesity treatments. The ONTIME study was registered at clinicaltrials.gov as NCT02829619. C1 [Garaulet, Marta; Vera, Beatriz; Bonnet-Rubio, Gemma; Gomez-Abellan, Purificacion] Univ Murcia, Dept Physiol, Chronobiol Lab, Murcia, Spain. [Garaulet, Marta; Vera, Beatriz; Bonnet-Rubio, Gemma; Gomez-Abellan, Purificacion] Res Biomed Inst Murcia IMIB, Murcia, Spain. [Lee, Yu-Chi; Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] Ctr Nacl Invest Cardiovasc, Dept Clin Invest, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac, Dept Nutr Genom, Madrid, Spain. RP Garaulet, M (reprint author), Univ Murcia, Dept Physiol, Chronobiol Lab, Murcia, Spain.; Garaulet, M (reprint author), Res Biomed Inst Murcia IMIB, Murcia, Spain. EM garaulet@um.es FU Spanish Government of Economy and Competitiveness [SAF2014-52480-R]; European Regional Development Fund; USDA Agricultural Research Service [58-1950-4-003]; Spanish Ministry of Economy and Competitiveness (MINECO); Pro-CNIC Foundation; MINECO [SEV-2015-0505] FX Supported by the Spanish Government of Economy and Competitiveness (SAF2014-52480-R) and the European Regional Development Fund (to MG) and the USDA Agricultural Research Service, under agreement 58-1950-4-003 (to JMO). The Centro Nacional Investigaciones Cardiovasculares (CNIC) is supported by the Spanish Ministry of Economy and Competitiveness (MINECO) and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). NR 47 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2016 VL 104 IS 4 BP 1160 EP 1166 DI 10.3945/ajcn.116.134528 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DY1SO UT WOS:000384874900028 PM 27629052 ER PT J AU Ahluwalia, N Herrick, KA Rossen, LM Rhodes, D Kit, B Moshfegh, A Dodd, KW AF Ahluwalia, Namanjeet Herrick, Kirsten A. Rossen, Lauren M. Rhodes, Donna Kit, Brian Moshfegh, Alanna Dodd, Kevin W. TI Usual nutrient intakes of US infants and toddlers generally meet or exceed Dietary Reference Intakes: findings from NHANES 2009-2012 SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE infants and toddlers; nationally representative sample; nutrient adequacy; recommended intakes; usual nutrient intake ID NATIONAL-HEALTH; PRESCHOOL-CHILDREN; FEEDING INFANTS; TOTAL-ENERGY; NUTRITION; COLLECTION; AMERICA; BIRTH; FOODS; B-24 AB Background: To our knowledge, few studies have described the usual nutrient intakes of US children aged <2 y or assessed the nutrient adequacy of their diets relative to the recommended Dietary Reference Intakes (DRIs). Objective: We estimated the usual nutrient intake of US children aged 6-23 mo examined in NHANES 2009-2012 and compared them to age-specific DRIs as applicable. Design: Dietary intake was assessed with two 24-h recalls for infants aged 6-11 mo (n = 381) and toddlers aged 12-23 mo (n = 516) with the use of the USDA's Automated Multiple-Pass Method. Estimates of usual nutrient intakes from food and beverages were obtained with the use of the National Cancer Institute method. The proportions of children with intakes below and above the DRI were also estimated. Results: The estimated usual intakes of infants were adequate for most nutrients; however, 10% had an iron intake below the Estimated Average Requirement (EAR), and only 21% had a vitamin D intake that met or exceeded the recommended Adequate Intake (AI). More nutrient inadequacies were noted among toddlers; 1 in 4 had a lower-than-recommended fat intake (percentage of energy), and most had intakes that were below the EAR for vitamins E (82%) and D (74%). Few toddlers (<1%) met or exceeded the AI for fiber and potassium. In contrast, 1 in 2 had sodium intakes that exceeded the Tolerable Upper Intake Level (UL); >= 16% and 41% of the children had excessive intakes (greater than the ULs) of vitamin A and zinc, respectively. Conclusions: The estimated usual intakes of infants were adequate for most nutrients. Most toddlers were at risk for inadequate intakes of vitamins D and E and had diets low in fiber and potassium. The sources contributing to excessive intakes of vitamin A and zinc among infants and toddlers may need further evaluation. C1 [Ahluwalia, Namanjeet; Herrick, Kirsten A.; Kit, Brian] CDC, Div Hlth & Nutr Examinat Survey, Natl Ctr Hlth Stat, Hyattsville, MD 20782 USA. [Rossen, Lauren M.] CDC, Off Anal & Epidemiol, Natl Ctr Hlth Stat, Hyattsville, MD USA. [Rhodes, Donna; Moshfegh, Alanna] USDA, Food Surveys Res Grp, Beltsville, MD 20705 USA. [Dodd, Kevin W.] NCI, NIH, Rockville, MD USA. RP Ahluwalia, N (reprint author), CDC, Div Hlth & Nutr Examinat Survey, Natl Ctr Hlth Stat, Hyattsville, MD 20782 USA. EM n.ahluwalia@cdc.gov NR 40 TC 1 Z9 1 U1 8 U2 8 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD OCT PY 2016 VL 104 IS 4 BP 1167 EP 1174 DI 10.3945/ajcn.116.137752 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DY1SO UT WOS:000384874900029 PM 27629049 ER PT J AU Bamberg, J del Rio, A AF Bamberg, John del Rio, Alfonso TI Accumulation of Genetic Diversity in the US Potato Genebank SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE AFLP; Accumulation; Genebank; Diversity ID WILD POTATO; MUTANT AB Efficient assessment of genetic diversity in a potato genebank includes understanding how much of the available genetic diversity has been captured in the genebank (from the wild or from other ex situ sources). We used existing datasets from three of the genebank's 93 species to model the increase of diversity over time, as measured by the addition of AFLP bands. DNA samples of a total of 411 populations of S. microdontum (mcd), S. jamesii (jam), and S. fendleri (fen) were analyzed. Assuming the first (oldest) populations as the baseline, the number of AFLP markers have increased by 128 % (mcd), 154 % (jam) and 111 % (fen) with addition of populations to the genebank. The pattern of actual observed chronological gain in diversity was similar to that which one would expect from random additions of populations except for fen, suggesting recent additions were somewhat redundant. All species continued to accumulate new loci at least up to 100 populations, jam particularly so. Much effort has been made over many years to thoroughly collect jam, so its 125 populations could serve as a model for the number of samples one might practically hope to acquire for the average species. Since 100 jam populations captured 95 % of its polymorphic loci, 100 populations might be set as an optimal benchmark. The average species in the genebank now has about 54 populations, so the size of the genebank would need to roughly double its capacity to optimize diversity according to the jam model. That would be a challenge, but probably not as limiting as the great time and resources needed to collect the germplasm. C1 [Bamberg, John; del Rio, Alfonso] USDA Agr Res Serv, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. RP Bamberg, J (reprint author), USDA Agr Res Serv, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov OI Del Rio, Alfonso/0000-0001-8780-747X NR 17 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD OCT PY 2016 VL 93 IS 5 BP 430 EP 435 DI 10.1007/s12230-016-9519-3 PG 6 WC Agronomy SC Agriculture GA DY5YL UT WOS:000385180800002 ER PT J AU Stulberg, MJ Rascoe, J Li, WB Yan, ZH Nakhla, MK Huang, Q AF Stulberg, Michael J. Rascoe, John Li, Wenbin Yan, Zonghe Nakhla, Mark K. Huang, Qi TI Development and Comparison of TaqMan-Based Real-Time PCR Assays for Detection and Differentiation of Ralstonia solanacearum strains SO CURRENT MICROBIOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; MULTIPLEX AB Bacterial wilt caused by Ralstonia solanacearum is destructive to many plant species worldwide. The race 3 biovar 2 (r3b2) strains of R. solanacearum infect potatoes in temperate climates and are listed as select agents by the U.S. government. TaqMan-based real-time quantitative PCR (qPCR) is commonly used in federal and state diagnostic laboratories over conventional PCR due to its speed and sensitivity. We developed the Rs16S primers and probe set and compared it with a widely used set (RS) for detecting R. solanacearum species complex strains. We also developed the RsSA3 primers and probe set and compared it with the previously published B2 and RsSA2 sets for specific detection of r3b2 strains. Both comparisons were done under standardized qPCR master mix and cycling conditions. The Rs16S and RS assays detected all 90 R. solanacearum species complex strains and none of the five outgroups, but the former was more sensitive than the latter. For r3b2 strain detection, the RsSA2 and RsSA3 sets specifically detected the 34 r3b2 strains and none of the 56 R. solanacearum non-r3b2 strains or out-group strains. The B2 set, however, detected five non-r3b2 R. solanacearum strains and was less sensitive than the other two sets under the same testing conditions. We conclude that the Rs16S, RsSA2, and RsSA3 sets are best suited under the standardized conditions for the detection of R. solanacearum species complex and r3b2 strains by TaqMan-based qPCR assays. C1 [Stulberg, Michael J.; Huang, Qi] ARS, Floral & Nursery Plants Res Unit, USDA, US Natl Arboretum, Beltsville, MD 20705 USA. [Stulberg, Michael J.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Rascoe, John; Li, Wenbin; Yan, Zonghe; Nakhla, Mark K.] Anim & Plant Hlth Inspect Serv, Ctr Plant Hlth Sci & Technol, USDA, Plant Protect & Quarantine, Beltsville, MD USA. RP Huang, Q (reprint author), ARS, Floral & Nursery Plants Res Unit, USDA, US Natl Arboretum, Beltsville, MD 20705 USA. EM qi.huang@ars.usda.gov FU U.S. Department of Agriculture (USDA), Agriculture Research Service (ARS); Animal and Plant Health Inspection Service (APHIS); U.S. Department of Energy (DOE); USDA; DOE [DE-AC05-06OR23100] FX This research was financially supported by the U.S. Department of Agriculture (USDA), Agriculture Research Service (ARS) and Animal and Plant Health Inspection Service (APHIS). It was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. NR 13 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 EI 1432-0991 J9 CURR MICROBIOL JI Curr. Microbiol. PD OCT PY 2016 VL 73 IS 4 BP 542 EP 549 DI 10.1007/s00284-016-1091-z PG 8 WC Microbiology SC Microbiology GA DY5ZJ UT WOS:000385184100012 PM 27402488 ER PT J AU Chang, PK Scharfenstein, LL Ehrlich, KC Diana Di Mavungu, J AF Chang, Perng-Kuang Scharfenstein, Leslie L. Ehrlich, Kenneth C. Diana Di Mavungu, Jose TI The Aspergillus flavus fluP-associated metabolite promotes sclerotial production SO FUNGAL BIOLOGY LA English DT Article DE Aflatoxin; Gene cluster; Polyketide synthase; Sclerotia ID FUNGAL SECONDARY METABOLISM; TRANSCRIPTION FACTOR FLBB; POLYKETIDE SYNTHASE GENE; ASEXUAL DEVELOPMENT; NIDULANS; AFLATOXIN; BIOSYNTHESIS; CLUSTERS; CONIDIATION; SPORULATION AB Aspergillus flavus is able to synthesize a variety of polyketide-derived secondary metabolites including the hepatocarcinogen, aflatoxin B-1. The fungus reproduces and disseminates predominantly by production of conidia. It also produces hardened mycelial aggregates called sclerotia that are used to cope with unfavourable growth environments. In the present study, we examined the role of A. flavus fluP, the backbone polyketide synthase gene of secondary metabolite gene cluster 41, on fungal development. The A. flavus CA14 fluP deletion mutant (Af Delta fluP) grew and accumulated aflatoxin normally but produced a lower amount of sclerotia than the parental strain. This was also true for the Aspergillus parasiticus BN9 fluP deletion mutant (Ap Delta fluP). The A. flavus fluP gene was positively regulated by developmental regulators of VeA and VelB but not by the global regulator of secondary metabolism, LaeA. Overexpression of fluP in Af Delta fluP (OEfluP) elevated its ability to produce sclerotia compared to that of the parental strain. Coculture of OEfluP with CA14, Af Delta fluP, Ap Delta fluP, or an A. flavus pptA deletion mutant incapable of producing functional polyketide synthases also allowed increased sclerotial production of the respective strains at edges where colonies made contact. Acetone extracts of OEfluP but not of Af Delta fluP exhibited the same effect in promoting sclerotial production of Af Delta fluP. These results suggest that FluP polyketide synthase is involved in the synthesis of a diffusible metabolite that could serve as a signal molecule to regulate sclerotiogenesis. Published by Elsevier Ltd on behalf of British Mycological Society. C1 [Chang, Perng-Kuang; Scharfenstein, Leslie L.; Ehrlich, Kenneth C.] ARS, Southern Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Diana Di Mavungu, Jose] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, 460 Ottergemsesteenweg, B-9000 Ghent, Belgium. RP Chang, PK (reprint author), ARS, Southern Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM perngkuang.chang@ars.usda.gov NR 48 TC 0 Z9 0 U1 9 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD OCT PY 2016 VL 120 IS 10 BP 1258 EP 1268 DI 10.1016/j.funbio.2016.07.010 PG 11 WC Mycology SC Mycology GA DY1LO UT WOS:000384856700009 PM 27647242 ER PT J AU Li, RJ Erpelding, JE AF Li, Ruijuan Erpelding, John E. TI Genetic diversity analysis of Gossypium arboreum germplasm accessions using genotyping-by-sequencing SO GENETICA LA English DT Article DE Genotyping-by-sequencing; Gossypium arboreum; Single nucleotide polymorphism; Genetic diversity ID GENOME-WIDE ASSOCIATION; POPULATION-STRUCTURE; COTTON; L.; HIRSUTUM; TOOL; MARKERS; RESISTANCE; SOFTWARE; PROGRAM AB The diploid cotton species Gossypium arboreum possesses many favorable agronomic traits such as drought tolerance and disease resistance, which can be utilized in the development of improved upland cotton cultivars. The USDA National Plant Germplasm System maintains more than 1600 G. arboreum accessions. Little information is available on the genetic diversity of the collection thereby limiting the utilization of this cotton species. The genetic diversity and population structure of the G. arboreum germplasm collection were assessed by genotyping-by-sequencing of 375 accessions. Using genome-wide single nucleotide polymorphism sequence data, two major clusters were inferred with 302 accessions in Cluster 1, 64 accessions in Cluster 2, and nine accessions unassigned due to their nearly equal membership to each cluster. These two clusters were further evaluated independently resulting in the identification of two sub-clusters for the 302 Cluster 1 accessions and three sub-clusters for the 64 Cluster 2 accessions. Low to moderate genetic diversity between clusters and sub-clusters were observed indicating a narrow genetic base. Cluster 2 accessions were more genetically diverse and the majority of the accessions in this cluster were landraces. In contrast, Cluster 1 is composed of varieties or breeding lines more recently added to the collection. The majority of the accessions had kinship values ranging from 0.6 to 0.8. Eight pairs of accessions were identified as potential redundancies due to their high kinship relatedness. The genetic diversity and genotype data from this study are essential to enhance germplasm utilization to identify genetically diverse accessions for the detection of quantitative trait loci associated with important traits that would benefit upland cotton improvement. C1 [Li, Ruijuan; Erpelding, John E.] USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd,POB 345, Stoneville, MS 38776 USA. RP Erpelding, JE (reprint author), USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd,POB 345, Stoneville, MS 38776 USA. EM John.Erpelding@ARS.USDA.GOV FU USDA-ARS [6066-22000-074-00D]; DOE [DE-AC05-06OR23100]; US Department of Energy (DOE); US Department of Agriculture (USDA) FX This research was funded by the USDA-ARS project 6066-22000-074-00D. This research was supported in part by an appointment to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the US Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 50 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD OCT PY 2016 VL 144 IS 5 BP 535 EP 545 DI 10.1007/s10709-016-9921-2 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DY2FV UT WOS:000384909400004 PM 27604991 ER PT J AU Petrini, J Iung, LHS Rodriguez, MAP Salvian, M Pertille, F Rovadoscki, GA Cassoli, LD Coutinho, LL Machado, PF Wiggans, GR Mourao, GB AF Petrini, J. Iung, L. H. S. Rodriguez, M. A. P. Salvian, M. Pertille, F. Rovadoscki, G. A. Cassoli, L. D. Coutinho, L. L. Machado, P. F. Wiggans, G. R. Mourao, G. B. TI Genetic parameters for milk fatty acids, milk yield and quality traits of a Holstein cattle population reared under tropical conditions SO JOURNAL OF ANIMAL BREEDING AND GENETICS LA English DT Article DE Correlation; dairy cattle; genomics; heritability; single nucleotide polymorphism; somatic cell score ID CONJUGATED LINOLEIC-ACID; DAIRY-CATTLE; BOVINE-MILK; GENOMIC PREDICTIONS; UNITED-STATES; FINAL SCORE; COWS; PEDIGREE; MASTITIS; HEALTH AB Information about genetic parameters is essential for selection decisions and genetic evaluation. These estimates are population specific; however, there are few studies with dairy cattle populations reared under tropical and sub-tropical conditions. Thus, the aim was to obtain estimates of heritability and genetic correlations for milk yield and quality traits using pedigree and genomic information from a Holstein population maintained in a tropical environment. Phenotypic records (n=36457) of 4203 cows as well as the genotypes for 57368 single nucleotide polymorphisms from 755 of these cows were used. Covariance components were estimated using the restricted maximum likelihood method under a mixed animal model, considering a pedigree-based relationship matrix or a combined pedigree-genomic matrix. High heritabilities (around 0.30) were estimated for lactose and protein content in milk whereas moderate values (between 0.19 and 0.26) were obtained for percentages of fat, saturated fatty acids and palmitic acid in milk. Genetic correlations ranging from -0.38 to -0.13 were determined between milk yield and composition traits. The smaller estimates compared to other similar studies can be due to poor environmental conditions, which may reduce genetic variability. These results highlight the importance in using genetic parameters estimated in the population under evaluation for selection decisions. C1 [Petrini, J.; Iung, L. H. S.; Rodriguez, M. A. P.; Salvian, M.; Pertille, F.; Rovadoscki, G. A.; Cassoli, L. D.; Coutinho, L. L.; Machado, P. F.; Mourao, G. B.] Univ Sao Paulo, Dept Anim Sci, Piracicaba, Brazil. [Wiggans, G. R.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA. RP Mourao, GB (reprint author), Univ Sao Paulo, Dept Anim Sci, ESALQ, BR-13418900 Piracicaba, SP, Brazil. EM gbmourao@usp.br RI Mourao, Gerson/D-2064-2012; Petrini, Juliana/R-6062-2016 OI Mourao, Gerson/0000-0002-0990-4108; FU FAPESP [2010/12929-6, 2012/15948-7]; CNPq; CAPES; Clinica do Leite; Department of Animal Science, Escola Superior de Agricultura 'Luiz de Queiroz' (University of Sao Paulo, Piracicaba - SP, Brazil) FX This research received the financial support of FAPESP (Proc 2010/12929-6 and Proc 2012/15948-7), CNPq and CAPES. The authors thank the 'Clinica do Leite' and Department of Animal Science, Escola Superior de Agricultura 'Luiz de Queiroz' (University of Sao Paulo, Piracicaba - SP, Brazil) for their support and for providing the database, and the Cooperative Dairy DNA Repository (CDDR) for providing the genotypes used as reference population in imputation analysis. NR 56 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2668 EI 1439-0388 J9 J ANIM BREED GENET JI J. Anim. Breed. Genet. PD OCT PY 2016 VL 133 IS 5 BP 384 EP 395 DI 10.1111/jbg.12205 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA DW4AT UT WOS:000383585000007 PM 26968150 ER PT J AU Hang, L Blum, AM Kumar, S Urban, JF Mitreva, M Geary, TG Jardim, A Stevenson, MM Lowell, CA Weinstock, JV AF Hang, Long Blum, Arthur M. Kumar, Sangeeta Urban, Joseph F., Jr. Mitreva, Makedonka Geary, Timothy G. Jardim, Armando Stevenson, Mary M. Lowell, Clifford A. Weinstock, Joel V. TI Downregulation of the Syk Signaling Pathway in Intestinal Dendritic Cells Is Sufficient To Induce Dendritic Cells That Inhibit Colitis SO JOURNAL OF IMMUNOLOGY LA English DT Article ID HELIGMOSOMOIDES-POLYGYRUS-BAKERI; EXCRETORY-SECRETORY PRODUCTS; ADAPTIVE IMMUNE-RESPONSES; T-CELLS; INFLAMMATORY RESPONSES; IL-10-DEFICIENT MICE; DEPENDENT PATHWAY; TYROSINE KINASE; INNATE IMMUNITY; CUTTING EDGE AB Helminthic infections modulate host immunity and may protect people in less-developed countries from developing immunological diseases. In a murine colitis model, the helminth Heligmosomoides polygyrus bakeri prevents colitis via induction of regulatory dendritic cells (DCs). The mechanism driving the development of these regulatory DCs is unexplored. There is decreased expression of the intracellular signaling pathway spleen tyrosine kinase (Syk) in intestinal DCs from H. polygyrus bakeri-infected mice. To explore the importance of this observation, it was shown that intestinal DCs from DC-specific Syk(-/-) mice were powerful inhibitors of murine colitis, suggesting that loss of Syk was sufficient to convert these cells into their regulatory phenotype. DCs sense gut flora and damaged epithelium via expression of C-type lectin receptors, many of which signal through the Syk signaling pathway. It was observed that gut DCs express mRNA encoding for C-type lectin (CLEC) 7A, CLEC9A, CLEC12A, and CLEC4N. H. polygyrus bakeri infection downmodulated CLEC mRNA expression in these cells. Focusing on CLEC7A, which encodes for the dectin-1 receptor, flow analysis showed that H. polygyrus bakeri decreases dectin-1 expression on the intestinal DC subsets that drive Th1/Th17 development. DCs become unresponsive to the dectin-1 agonist curdlan and fail to phosphorylate Syk after agonist stimulation. Soluble worm products can block CLEC7A and Syk mRNA expression in gut DCs from uninfected mice after a brief in vitro exposure. Thus, downmodulation of Syk expression and phosphorylation in intestinal DCs could be important mechanisms through which helminths induce regulatory DCs that limit colitis. C1 [Hang, Long; Blum, Arthur M.; Kumar, Sangeeta; Weinstock, Joel V.] Tufts Med Ctr, Div Gastroenterol Hepatol, Dept Internal Med, Boston, MA 02111 USA. [Urban, Joseph F., Jr.] ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, USDA, Beltsville, MD 20705 USA. [Mitreva, Makedonka] Washington Univ, Sch Med, Genome Inst, St Louis, MO 63108 USA. [Geary, Timothy G.; Jardim, Armando] McGill Univ, Inst Parasitol, Ste Anne De Bellevue, PQ H9X 3V9, Canada. [Stevenson, Mary M.] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada. [Stevenson, Mary M.] McGill Univ, Dept Med, Montreal, PQ H4A 3J1, Canada. [Lowell, Clifford A.] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA. RP Weinstock, JV (reprint author), Tufts Med Ctr, Div Gastroenterol Hepatol, 800 Washington St,233, Boston, MA 02111 USA. EM jweinstock2@tuftsmedicalcenter.org FU NIAID NIH HHS [R01 AI065495, R01 AI068150, R01 AI113272]; NIDDK NIH HHS [R01 DK038327, R01 DK058755, R01 DK091987, R56 DK058755] NR 63 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD OCT 1 PY 2016 VL 197 IS 7 BP 2948 EP 2957 DI 10.4049/jimmunol.1600063 PG 10 WC Immunology SC Immunology GA DY3OX UT WOS:000385004600043 PM 27559049 ER PT J AU Jansky, S Fajardo, D AF Jansky, Shelley Fajardo, Diego TI Amylose content decreases during tuber development in potato SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE Solanum tuberosum; tuber starch; potato; amylose ID METABOLIC VARIABLES; AMYLOPECTIN STARCH; CULTIVARS; GROWTH; QUALITY; HEALTH; SIZE; L. AB BACKGROUND: Potato starch is composed primarily of amylopectin and amylose in an approximately 3:1 ratio. Amylose is considered to be nutritionally desirable in North American and European markets, so there is interest in finding strategies to increase the amylose content of potato starch. There is also interest in marketing 'baby' potatoes, which are harvested when they are physiologically immature. This study was carried out to determine weekly changes in amylose content in potato tubers of 11 North American cultivars during the growing season. The trial was repeated across 3 years. RESULTS: We determined that amylose content is highest early and it decreases in a linear fashion as the growing season progresses. Mean amylose content across cultivars and years declined from 30.0% in late June to 26.8% in late August. The rate of decrease varied across years, with slopes of linear regression plots ranging from -0.17 in 2012 to -0.74 in 2011. Amylose content in tuber starch varied among cultivars, with the highest levels observed in Ranger Russet (30.7%) and White Pearl (31.6%); it was lowest in Kennebec (25.7%) and Langlade (25.6%). CONCLUSIONS: This study adds to a growing body of literature on the nutritional value of immature potato tubers. In addition to having higher levels of some phytonutrients, as reported in other studies, immature tubers have a higher proportion of amylose in the starch. This is nutritionally desirable in affluent regions where high fiber content is more important than calories from carbohydrates. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Jansky, Shelley; Fajardo, Diego] USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. [Jansky, Shelley] USDA ARS, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. RP Jansky, S (reprint author), USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov FU USDA-ARS Research Associate Program; Crop germplasm Committee of the National Plant Germ plasm System FX Partial funding for the senior author's salary was provided by the USDA-ARS Research Associate Program and the Crop germplasm Committee of the National Plant Germ plasm System. NR 33 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD OCT PY 2016 VL 96 IS 13 BP 4560 EP 4564 DI 10.1002/jsfa.7673 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DV3QO UT WOS:000382838100033 PM 26931799 ER PT J AU Yoo, CG Nghiem, NP Kim, TH AF Yoo, Chang Geun Nghiem, Nhuan P. Kim, Tae Hyun TI Production of fermentable sugars from corn fiber using soaking in aqueous ammonia (SAA) pretreatment and fermentation to succinic acid using Escherichia coli AFP184 SO KOREAN JOURNAL OF CHEMICAL ENGINEERING LA English DT Article DE Corn Fiber; Ammonia Pretreatment; Succinic Acid; Fermentable Sugars ID FUEL ETHANOL; BARLEY STRAW; FRACTIONATION AB Conversion of corn fiber (CF), a by-product from the corn-to-ethanol conversion process, into fermentable sugar and succinic acid was investigated using soaking in aqueous ammonia (SAA) pretreatment followed by biological conversions, including enzymatic hydrolysis and fermentation using genetically engineered E. coli (AFP184). The SAA pretreatment (using a 15% w/w NH4OH solution at a solid-to-liquid ratio of 1: 10 at 60 A degrees C for 24 h) removed 20-38% of lignin and significantly improved the digestibility of the treated solid (85-99% of glucan digestibility). Following the enzymatic hydrolysis, the sugar-rich hydrolysate was subjected to dilute sulfuric acid treatment (1 wt% sulfuric acid and 120 A degrees C for 1 h), which hydrolyzed the oligosaccharides in the hydrolysate into fermentable monomeric sugars. The mixed sugar hydrolysates containing hexose and pentose obtained from the two-step hydrolysis and SAA pretreatment were fermented to succinic acid using a genetically engineered microorganism, Escherichia coli AFP184, for evaluating the fermentability. Engineered E. coli AFP184 effectively converted soluble sugars in the hydrolysate to succinic acid (20.7 g/L), and the production rate and yield were further enhanced with additional nutrients; the highest concentration of succinic acid was 26.3 g/L for 48 h of fermentation. C1 [Yoo, Chang Geun] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Nghiem, Nhuan P.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Kim, Tae Hyun] Kongju Natl Univ, Dept Environm Engn, Cheonan 31080, Chungnam, South Korea. RP Kim, TH (reprint author), Kongju Natl Univ, Dept Environm Engn, Cheonan 31080, Chungnam, South Korea. EM thkim@kongju.ac.kr FU Kongju National University FX This work was supported by a research grant from Kongju National University in 2015. Gerrard Senske and Justin Montanti provided invaluable assistance to the experimental efforts on succinic acid fermentation. NR 17 TC 0 Z9 0 U1 12 U2 12 PU KOREAN INSTITUTE CHEMICAL ENGINEERS PI SEOUL PA F.5, 119, ANAM-RO, SEONGBUK-GU, SEOUL 136-075, SOUTH KOREA SN 0256-1115 EI 1975-7220 J9 KOREAN J CHEM ENG JI Korean J. Chem. Eng. PD OCT PY 2016 VL 33 IS 10 BP 2863 EP 2868 DI 10.1007/s11814-016-0139-x PG 6 WC Chemistry, Multidisciplinary; Engineering, Chemical SC Chemistry; Engineering GA DY5LG UT WOS:000385140600011 ER PT J AU Koroglu, A Baldemir, A Ozek, G Bedir, E Tabanca, N Ali, A Khan, IA Baser, KHC Ozek, T AF Koroglu, Aysegul Baldemir, Ayse Ozek, Gulmira Bedir, Erdal Tabanca, Nurhayat Ali, Abbas Khan, Ikhlas A. Baser, Kemal Husnu Can Ozek, Temel TI 11-Hydroxy-2,4-cycloeudesmane from the Leaf Oil of Juglans regia and Evaluation of its Larvicidal Activity SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Juglans regia; Essential oil; Larvicidal activity; Fractionation; Preparative gas chromatography; NMR ID AEDES-AEGYPTI; TRADITIONAL MEDICINE; WALNUT; LEAVES; PLANTS; CONSTITUENTS; ANTIOXIDANT; EXTRACTION; TURKEY; L. AB Leaves of Juglans regia L. collected from two different locations [Adana (A) and Ankara (BA from Turkey were subjected to hydrodistillation in a Clevenger type apparatus to yield the essential oils (EOs). GC/MS and GC-FID analyses revealed that the A EO was rich in thymol (23.1%), while caryophyllene oxide (33.8%) was found as the main constituent of B EO. Both contained beta-eudesmol (1.4% - 9.5%), (E)-geranyl acetone (3.7% - 5.8%) and the eudesmane type constituent juglaterpene A (3.1% - 11.0%). Using a HP Innowax preparative capillary column connected to a preparative fraction collector, an unknown constituent, juglaterpene A (1, 11-hydroxy-2,4-cycloeudesmane), was isolated in a rapid one-step manner with > 94.0% purity. Structure determination was accomplished from 1D- and 2D-NMR spectroscopic data. Oil B demonstrated significant larvicidal activity against 1st instar Aedes aegypti L. C1 [Koroglu, Aysegul] Ankara Univ, Fac Pharm, Dept Pharmaceut Bot, TR-06100 Ankara, Turkey. [Baldemir, Ayse] Erciyes Univ, Fac Pharm, Dept Pharmaceut Bot, TR-38039 Melikgazi Kayseri, Turkey. [Ozek, Gulmira; Ozek, Temel] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. [Bedir, Erdal] Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Izmir, Turkey. [Tabanca, Nurhayat; Ali, Abbas; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Tabanca, Nurhayat] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Tabanca, Nurhayat] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Baser, Kemal Husnu Can] Near East Univ, Fac Pharm, Deta Pharmacognosy, Nicosia, Cyprus. RP Ozek, G (reprint author), Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. EM gulmiraozek@gmail.com RI Baser, K. Husnu Can/C-3109-2008 FU Anadolu University Scientific Research Project (BAP project) [090322]; Deployed War-Fighter Protection Research Program Grant; U.S. Department of Defense through the Armed Forces Pest Management Board FX Authors thank Anadolu University Scientific Research Project (BAP project No090322) for supporting this research. This study, in part by the Deployed War-Fighter Protection Research Program Grant, was funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Dr Bharathi Avula (NCNPR, University of Mississippi, University, MS) for HR ESI-TOF-MS analysis and Dr James J. Becnel, (USDA-ARS, CMAVE, Gainesville FL), for supplying Ae. aegypti eggs. NR 24 TC 0 Z9 0 U1 6 U2 6 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1421 EP 1424 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900005 ER PT J AU Tabanca, N AF Tabanca, Nurhayat TI Chemical Composition of Fruit Essential Oil of Endemic Malabaila pastinacifolia SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Malabaila pastinacifolia; Aliphatic esters; Hexyl butyrate; Hexyl hexanoate ID MEDICINAL-PLANTS; BOISS.; TURKEY AB The water distilled essential oil (EO) from the fruits of Malabaila pastinacifolia Boiss & Balansa (Apiaceae), an endemic species from Turkey, was analyzed using GC-FID and GC-MS systems. The analysis revealed 38 compounds accounting for 97.6% of the EO. Hexyl butyrate (47.2 %), hexyl hexanoate (22.7%) and a-terpinyl acetate (11.1 %) were found as the main constitutes. This is the first report on the EO composition of M. pastinacifolia. C1 [Tabanca, Nurhayat] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Tabanca, Nurhayat] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Tabanca, N (reprint author), Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA.; Tabanca, N (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM ntabanca@ufl.edu NR 17 TC 0 Z9 0 U1 9 U2 9 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1511 EP 1512 PG 2 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900024 ER PT J AU Tabanca, N Nalbantsoy, A Bernier, UR Agramonte, NM Ali, A Li, AY Yalcin, HT Gucel, S Demirci, B AF Tabanca, Nurhayat Nalbantsoy, Ayse Bernier, Ulrich R. Agramonte, Natasha M. Ali, Abbas Li, Andrew Y. Yalcin, Husniye Tansel Gucel, Salih Demirci, Betul TI Essential Oil Composition of Pimpinella cypria and its Insecticidal, Cytotoxic, and Antimicrobial Activity SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Pimpinella cypria; Phenylpropanoid; Aedes aegypti; Amblyomma americanum; Cytotoxicity; Antimicrobial ID MASS SPECTROMETRIC ANALYSIS; CANCER-CELLS; TURKEY; REPELLENT; TOXICITY AB A water-distilled essential oil (EO) from the aerial parts of Pimpinella cypria Boiss. (Apiaceae), an endemic species in northern Cyprus, was analyzed by GC-FID and GC-MS. Forty-five compounds were identified in the oil, which comprised 81.7% of the total composition. The compound classes in the oil were oxygenated sesquiterpenes (33.9%), sesquiterpenes (22.0%), monoterpenes (11.4%), oxygenated monoterpenes (2.6%), and phenylpropanoids (7.5%). The main components of the oil were (Z)-beta-farnesene (6.0%), spathulenol (5.9%), ar-curcumene (4.3%), and 1,5-epoxy-salvial(4)14-ene (3.8%). The P. cypria EO deterred yellow fever mosquitoes (Aedes aegypti) from biting at a concentration of 10 mu g/cm(2) in in vitro bioassays. The oil was tested for repellency in assays using human volunteers. The oil had a minimum effective dosage (MED) for repellency of 47 +/- 41 mu g/cm(2) against Ae. aegypti, which was less efficacious than the positive control N,N-diethyl-3-methylbenzamide (DEET). In larval bioassays, P. cypria EO showed an LC50 value of 28.3 ppm against 1st instar Ae. aegypti larvae. P. cypria EO demonstrated dose dependent repellency against nymphs of the lone star tick, Amblyomma americanum. Between 45.0% and 85.0% repellency was observed at concentrations ranging from 26 to 208 mu g/cm(2). However, P. cypria EO was less effective compared with DEET in the tick bioassays. Cytotoxicity assays showed that the P. cypria EO did not exhibit significant effects up to the maximum treatment concentration of 50 mu g/mL on HEK293, PC3, U87MG, and MCF cells. P. cypria EO also demonstrated moderate antimicrobial activity against Gram-negative and-positive bacteria with MICs ranging from 15.6 to 62.5 mu g/mL, except for Candida albicans, which showed the same MIC value of 7.8 mu g/mL as the positive control, flucytosine. This is the first report on the chemical composition of P. cypria EO and its insecticidal, toxicant, cytotoxic, and antimicrobial activity. C1 [Tabanca, Nurhayat; Ali, Abbas] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Tabanca, Nurhayat; Agramonte, Natasha M.] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Nalbantsoy, Ayse] Ege Univ, Fac Engn, Dept Bioengn, TR-35100 Izmir, Turkey. [Tabanca, Nurhayat; Bernier, Ulrich R.; Agramonte, Natasha M.] USDA ARS, Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Li, Andrew Y.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Yalcin, Husniye Tansel] Ege Univ, Fac Sci, Dept Biol, Basic & Ind Microbiol Sect, TR-35100 Izmir, Turkey. [Gucel, Salih] Near East Univ, Sci Educ Dept, CY-33010 Nicosia, Northern Cyprus, Cyprus. [Demirci, Betul] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26470 Eskisehir, Turkey. RP Tabanca, N (reprint author), Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA.; Tabanca, N (reprint author), Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA.; Tabanca, N; Bernier, UR (reprint author), USDA ARS, Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM ntabanca@ufl.edu; uli.bernier@ars.usda.gov FU Deployed War-Fighter Protection Research Program Grant by the U.S. Department of Defense through the Armed Forces Pest Management Board FX We would like to thank AREL (Ege University School of Medicine Research and Education Laboratory) for carrying out the cell culture studies. This study was in part funded by a Deployed War-Fighter Protection Research Program Grant by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Dr James J. Becnel (USDA-ARS, CMAVE, Gainesville FL) for supplying Ae. aegypti eggs, and Greg Allen (USDA-ARS, CMAVE, Gainesville FL) and James McCrary (USDA, ARS, IIBBL) for mosquito and tick repellency bioassays. NR 25 TC 0 Z9 0 U1 3 U2 3 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1531 EP 1534 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900030 ER PT J AU Ali, A Tabanca, N Amin, E Demirci, B Khan, IA AF Ali, Abbas Tabanca, Nurhayat Amin, Elham Demirci, Betul Khan, Ikhlas A. TI Chemical Composition and Biting Deterrent Activity of Essential Oil of Tagetes patula (Marigold) against Aedes aegypti SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Asteraceae; Tagetes panda; beta-Caryophyllene; Caryophyllene oxide; Spathulenol; Aedes aegypti; Mosquito ID DIPTERA-CULICIDAE; ANTIFUNGAL ACTIVITY; LARVICIDAL ACTIVITY; ROOT CULTURES; MOSQUITO; ASTERACEAE; LARVAE; PLANT; BITHIOPHENES; BENZOFURANS AB The hydrodistilled leaf essential oil (EO) of Tagetes panda L. was analyzed by GC-FID and GC-MS. The main components of the oil were characterized as caryophyllene oxide (18.4%), beta-caryophyllene (18.0%) and spathulenol (9.1%). The EO was screened for its biting deterrent activity against Aedes aegypti L. using the in vitro K&D module system. T. panda EO exhibited good biting deterrent activity. The results suggest that these sesquitervenes may contribute to biting deterrent activity, but the role of minor components cannot be excluded. T. panda EO also showed 100, 90 and 10% mortality at dosages of 125, 62.5 and 31.25 ppm, respectively, in 1-day-old larvae of Ae. aegypti. C1 [Ali, Abbas; Tabanca, Nurhayat; Amin, Elham; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Tabanca, Nurhayat] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Tabanca, Nurhayat] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Amin, Elham] Beni Suef Univ, Fac Pharm, Bani Suwayf, Egypt. [Amin, Elham] AL Jouf Univ, Fac Pharm, Sakakah, Saudi Arabia. [Demirci, Betul] Anadolu Univ, Fac Pharm, Dept Pharmacognosy, TR-26140 Eskisehir, Turkey. RP Ali, A (reprint author), Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. EM aali@olemiss.edu FU USDA/ARS [56-6402-1-612]; U.S. Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by USDA/ARS grant No. 56-6402-1-612 and a Deployed War-Fighter Protection research program grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Dr James J. Becnel, Mosquito and Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, for supplying Ae. aegypti eggs. NR 35 TC 0 Z9 0 U1 2 U2 2 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1535 EP 1538 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900031 ER PT J AU Ozek, G Tabanca, N Radwan, MM Shatar, S Altantsetseg, A Baatar, D Baser, KHC Becnel, JJ Ozek, T AF Ozek, Gulmira Tabanca, Nurhayat Radwan, Mohammed M. Shatar, Sanduin Altantsetseg, Altaa Baatar, Dumaajav Baser, Kemal H. C. Becnel, James J. Ozek, Temel TI Preparative Capillary GC for Characterization of Five Dracocephalum Essential Oils from Mongolia, and their Mosquito Larvicidal Activity SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Dracocephalum essential oils; Preparative gas chromatography; NMR; Limonen-10-al; Aedes aegypti; Mosquito control ID CHEMICAL-COMPOSITION; BIOLOGICAL-ACTIVITY; HETEROPHYLLUM; KOTSCHYI; IRAN; SUBCAPITATUM; CONSTITUENTS AB The chemical composition and mosquito larvicidal and adulticidal activity of five essential oils (EOs),Dracocephalum ruyschiana L. (DR), D. foetidum Bunge (DF), D. moldavica L. (DM), D. fruticztlosum Steph. ex Willd. (DFr) and D. peregrinum L. (DP) were evaluated. Simultaneous GC-FID and GC-MS analyses revealed in the EOs of DR, DF and DM an unidentified compound (1) ([M(+)122], 5.4%, 57.9% and 74.0%, respectively). Therefore, we aimed to isolate compound 1 using Preparative Capillary GC (PCGC) connected to a Preparative Fraction Collector (PFC) system. Structure determination of 1 was determined by H-1- and C-13-NMR as p-mentha-1,8-dien-10-al (1, limonen-10-a1). Other detected major constituents were thymol (34.0%) and carvacrol (6.1%) in DR; limonene (28.8%) in DF; cis-chrysanthenol acetate (29.1%) and trans-verbenol (5.0%) in DFr; and linalool (17.9%), trans-beta-bergamotene (7.9%), (E)-nerolidol (7.7%) and eugenol (5.5%) in DP. The DFr, DP, DR and DM EOs produced 100% mortality to 1st instar larvae of Aedes aegypti L. at 250 ppm and DR and DM EO exhibited the strongest activity and killed 100% 1st instar larvae at 62.5 ppm. No adulticidal activity was observed against female Ae. aegypti. In the scope of the present study, for the first time isolation of limonen-10-al (1) from DR, DM, and DP EOs and larvicidal activity of five Dracocephalum EOs were reported. C1 [Ozek, Gulmira; Baser, Kemal H. C.; Ozek, Temel] Anadolu Univ, Dept Pharmacognosy, Fac Pharm, TR-26470 Eskisehir, Turkey. [Tabanca, Nurhayat; Radwan, Mohammed M.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Tabanca, Nurhayat] Univ Florida, Emerging Pathogens Inst, Dept Entomol & Nematol, Gainesville, FL 32610 USA. [Tabanca, Nurhayat; Becnel, James J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Shatar, Sanduin; Altantsetseg, Altaa] MAS, Inst Chem & Chem Technol, Ulaanbaatar 211051, Mongol Peo Rep. [Baatar, Dumaajav] Mongolian Acad Sci, Inst Biol, Ulaanbaatar 211051, Mongol Peo Rep. [Baser, Kemal H. C.] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia. [Baser, Kemal H. C.] Near East Univ, Fac Pharm, Dept Pharmacognosy, Nicosia, Cyprus. RP Ozek, G (reprint author), Anadolu Univ, Dept Pharmacognosy, Fac Pharm, TR-26470 Eskisehir, Turkey. EM gulmiraozek@gmail.com RI Baser, K. Husnu Can/C-3109-2008 FU Anadolu University Scientific Research Project (BAP) [090322]; Mongolian Academy of Sciences; Deployed War-Fighter Protection Research Program Grant - U.S. Department of Defense through the Armed Forces Pest Management Board FX We thank Anadolu University Scientific Research Project (BAP project No 090322) and the Mongolian Academy of Sciences for supporting this research. This study was partly supported by the Deployed War-Fighter Protection Research Program Grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. Thanks to Dr William Reid (USDA-ARS, CMAVE) for the mosquito bioassays. NR 22 TC 0 Z9 0 U1 4 U2 4 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1541 EP 1544 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900033 ER PT J AU Wang, XN Wedge, DE Cutler, SJ AF Wang, Xiaoning Wedge, David E. Cutler, Stephen J. TI Chemical and Biological Study of Cladosporin, an Antimicrobial Inhibitor: A Review SO NATURAL PRODUCT COMMUNICATIONS LA English DT Review DE Cladosporium cladosporioides; Cladosporin; Antifungal; Antimalarial; Plasmodium falciparum; Lysyl-tRNA Synthetase; Herbicides ID TRANSFER-RNA SYNTHETASE; PLASMODIUM-FALCIPARUM; POLYKETIDE BIOSYNTHESIS; STRUCTURAL BASIS; FATTY-ACID; METABOLITES; DERIVATIVES; FUNGUS; STEREOCHEMISTRY; ISOCLADOSPORIN AB Natural antifungal agents are generally broad-spectrum compounds with low mammalian and environmental toxicity. Cladosporin is a naturally occurring fungal metabolite mainly isolated from the endophytic fungus Cladosporium cladosporioides. This review article summarizes the chemistry and biological properties of cladosporin covering references published from 1971-2016, including the source, phytochemical characterization, biosynthesis, total synthesis, structure and activity (SAR), and biological activity of cladosporin. Cladosporin exhibited potent antibacterial, antifungal, insecticidal, and anti-inflammatory activities, as well as plant growth regulatory effects. More importantly, cladosporin was identified as having potent, nanomolar, antiparasitic activity against both Plasmodium falciparum blood and liver stages via specific inhibition of protein synthesis. This provides a new approach for the design of isocoumarin-based compounds for the treatment of malaria. Herbicidal activity and antifungal activity against Oyptococcus neoformans (IC50 value of 17.7 mu g/mL) of cladosporin are also described here in the review for the first time. Cladosporin selectively inhibited the growth of a monocot (agostis) and showed no activity against a dicot (lettuce), which indicates its great potential as a selective herbicide for monocots in agriculture use. The above data suggest that cladosporin has great potential utility as a lead compound in the development of agrochemicals against certain plant pathogens and pharmaceuticals against drug-resistant bacteria and parasites. C1 [Wang, Xiaoning] Univ Notre Dame, Chem Synth & Drug Discovery Facil, Notre Dame, IN 46556 USA. [Wang, Xiaoning] Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA. [Wedge, David E.] Univ Mississippi, ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Cutler, Stephen J.] Univ Mississippi, Dept BioMol Sci, University, MS 38677 USA. [Cutler, Stephen J.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. RP Wang, XN (reprint author), Univ Notre Dame, Chem Synth & Drug Discovery Facil, Notre Dame, IN 46556 USA.; Wang, XN (reprint author), Univ Notre Dame, Dept Chem & Biochem, Notre Dame, IN 46556 USA. EM xwang33@nd.edu NR 50 TC 0 Z9 0 U1 9 U2 9 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X EI 1555-9475 J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD OCT PY 2016 VL 11 IS 10 SI SI BP 1595 EP 1600 PG 6 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA DX6SR UT WOS:000384513900042 ER PT J AU Bochkov, AV Valim, MP Ochoa, R OConnor, BM Averianov, AO AF Bochkov, Andre V. Valim, Michel P. Ochoa, Ronald OConnor, Barry M. Averianov, Alexander O. TI Origin of host-parasite associations of Marsupialges misonnei (Acariformes: Psoroptidae)-a parasitological detective story SO PARASITOLOGY RESEARCH LA English DT Article DE Acari; Bare-tailed woody opossum; Coatis; Ectoparasites; Host shifts; Mites ID ACARIFORMES ASTIGMATA; MORPHOLOGY; PHYLOGENY AB Host associations of permanent ectoparasitic mite Marsupialges misonnei Fain, 1963 (Acariformes: Psoroptidae: Marsupialginae) are analyzed. This species was first recorded from an ethanol-preserved museum specimen of Caluromys philander (Linnaeus, 1758) (Didelphimorphia: Didelphidae) originating from French Guiana. We discovered specimens of M. misonnei from both species known in the carnivore genus Nasua (Carnivora: Procyonidae): N. narica (Linnaeus, 1766) from Panama (collected in the field) and N. nasua (Linnaeus, 1766) from Brazil (collected from dry museum specimen). Two alternative hypotheses about an initial host of this mite (bare-tailed woody opossum or coatis) are discussed. We argue that M. misonnei was originally parasitic on Nasua spp. and occasionally contaminated C. philander from these hosts in the collecting process. C1 [Bochkov, Andre V.; Averianov, Alexander O.] Russian Acad Sci, Inst Zool, Univ Skaya Embankment 1, St Petersburg 199034, Russia. [Bochkov, Andre V.; OConnor, Barry M.] Univ Michigan, Museum Zool, 1109 Geddes Ave, Ann Arbor, MI 48109 USA. [Valim, Michel P.] Univ Sao Paulo, Museu Zool, Ave Nazare 481, BR-04263000 Sao Paulo, SP, Brazil. [Ochoa, Ronald] USDA ARS, BARC West, 10300 Baltimore Ave,Bldg 012,5th St, Beltsville, MD 20705 USA. RP Bochkov, AV (reprint author), Russian Acad Sci, Inst Zool, Univ Skaya Embankment 1, St Petersburg 199034, Russia.; Bochkov, AV (reprint author), Univ Michigan, Museum Zool, 1109 Geddes Ave, Ann Arbor, MI 48109 USA. EM andrevbochkov@gmail.com RI Museu de Zoologia da USP, MZ-USP/Q-2192-2016 FU Russian Foundation for Basic Research (RFBR) [15-29-02533_ophiN_M]; Sao Paulo Research Foundation-FAPESP [2011/11420-5, 2012/06951-4] FX We thank Dr. Alexander Stekolnikov (Zoological Institute RAS, St. Petersburg, Russia) for valuable comments and the curator Mario de Vivo and his technician Juliana Gualda for permitting the access of MPV for search fur mites on skins in the mastozoological collection of the MZUSP. This research was supported by a grant from the Russian Foundation for Basic Research (RFBR No 15-29-02533_o phi Nu_M) to AVB and by a grant from the Sao Paulo Research Foundation-FAPESP (2011/11420-5; 2012/06951-4) to MPV. NR 9 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD OCT PY 2016 VL 115 IS 10 BP 3695 EP 3698 DI 10.1007/s00436-016-5192-1 PG 4 WC Parasitology SC Parasitology GA DY5ME UT WOS:000385143100004 PM 27465562 ER PT J AU Boyd, JN Raymond, GA Call, GP Pistrang, MJ AF Boyd, Jennifer N. Raymond, Gregory A. Call, Geoff P. Pistrang, Mark J. TI Ecophysiological performance of the rare terrestrial orchid Platanthera integrilabia across contrasting habitats SO PLANT ECOLOGY LA English DT Article DE Orchid; Ecophysiology; Photosynthesis; Platanthera integrilabia; Rare species ID LOCAL ADAPTATION; SHADE TOLERANCE; USE EFFICIENCY; PLANTS; FOREST; PHOTOSYNTHESIS; POPULATIONS; TRAITS; SEEDS; TAXA AB Platanthera integrilabia is a rare terrestrial orchid species generally associated with semiopen forested wetlands in the southeastern US. It has been suggested that P. integrilabia has restrictive abiotic resource requirements; however, these requirements have not been implicitly studied despite their potential application to species conservation. We investigated the influence of light and soil moisture on P. integrilabia at landscape and local scales and population and organismal levels across and within four occurrences with contrasting canopy openness. We also evaluated the potential for leaf-level physiological responses to light and soil moisture to reflect habitat suitability and influence performance. At landscape scales, light and soil moisture were not associated with P. integrilabia density or individual size. Across sites, measures of photosynthetic light response and water-use efficiency indicated that P. integrilabia can maximize photosynthetic efficiency and energy gain in contrasting light and soil moisture environments. Minimal associations of these measures with abiotic variations within sites suggested that the capacity for adjustments across small spatial and/or temporal scales may be limited. Preservation of existing habitats associated with viable P. integrilabia occurrences is warranted, but the leaf-level ecophysiology of this species indicates that its habitat suitability also could include more open and drier sites. For populations of P. integrilabia experiencing declines in habitats with recent disturbance, we suggest the possibility that well-managed modifications to canopy cover may be beneficial. C1 [Boyd, Jennifer N.; Raymond, Gregory A.] Univ Tennessee, Dept Biol Geol & Environm Sci, 615 McCallie Ave, Chattanooga, TN 37403 USA. [Call, Geoff P.] US Fish & Wildlife Serv, US Dept Interior, Tennessee Ecol Serv Field Off, 446 Neal St, Cookeville, TN 38501 USA. [Pistrang, Mark J.] US Forest Serv, USDA, Cherokee Natl Forest, Cleveland, TN 37312 USA. RP Boyd, JN (reprint author), Univ Tennessee, Dept Biol Geol & Environm Sci, 615 McCallie Ave, Chattanooga, TN 37403 USA. EM jennifer-boyd@utc.edu FU U.S. Fish and Wildlife Service; University of Tennessee at Chattanooga FX The U.S. Fish and Wildlife Service provided the primary funding support for this project. The University of Tennessee at Chattanooga provided supplemental funding and logistical support. We thank Adam Dattilo, Tara Littlefield, and Marie Tackett for orienting us to the study sites, assisting us with site access permissions, and providing invaluable information about our study species from their personnel experiences. We also appreciate Matt Richards for sharing his experience with ongoing species conservation efforts. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service or the U.S. Forest Service. NR 40 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD OCT PY 2016 VL 217 IS 10 BP 1259 EP 1272 DI 10.1007/s11258-016-0653-2 PG 14 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DY6DX UT WOS:000385197700008 ER PT J AU Cho, IS Igori, D Lim, S Choi, GS Hammond, J Lim, HS Moon, JS AF Cho, In-Sook Igori, Davaajargal Lim, Seungmo Choi, Gug-Seoun Hammond, John Lim, Hyoun-Sub Moon, Jae Sun TI Deep Sequencing Analysis of Apple Infecting Viruses in Korea SO PLANT PATHOLOGY JOURNAL LA English DT Article DE apple viruses; deep sequencing; virome ID MOSAIC-VIRUS; FRUIT-TREES; RNAS; IDENTIFICATION; FOVEAVIRUS; POLYMERASE; DIVERSITY; DISEASE; ISOLATE; GENOME AB Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes. Sequences of ASPV and ASGV were the most abundantly represented by the 52 contigs assembled. The presence of the five viruses in the samples was confirmed by RT-PCR using specific primers based on the sequences of each assembled contig. All five viruses were detected in three of the samples, whereas all samples had mixed infections with at least two viruses. The most frequently detected virus was ASPV, followed by ASGV, ApLV, ACLSV, and AGCaV which were withal found in mixed infections in the tested samples. AGCaV was identified in assembled contigs ID 1012480 and 93549, which showed 82% and 78% nt sequence identity with ORF1 of AGCaV isolate Aurora-1. ApLV was identified in three assembled contigs, ID 65587, 1802365, and 116777, which showed 77%, 78%, and 76% nt sequence identity respectively with ORF1 of ApLV isolate LA2. Deep sequencing assay was shown to be a valuable and powerful tool for detection and identification of known and unknown virome in infected apple trees, here identifying ApLV and AGCaV in commercial orchards in Korea for the first time. C1 [Cho, In-Sook; Choi, Gug-Seoun] Natl Inst Hort & Herbal Sci, Hort & Herbal Crop Environm Div, Rural Dev Adm, Wonju 55365, South Korea. [Igori, Davaajargal; Lim, Seungmo; Moon, Jae Sun] Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 34141, South Korea. [Igori, Davaajargal; Lim, Seungmo; Moon, Jae Sun] Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 34113, South Korea. [Hammond, John] USDA ARS, Floral & Nursery Plants Unit, US Natl Arboretum, Beltsville, MD 20705 USA. [Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Daejeon 34134, South Korea. RP Moon, JS (reprint author), Korea Res Inst Biosci & Biotechnol, Mol Biofarming Res Ctr, Daejeon 34141, South Korea.; Moon, JS (reprint author), Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 34113, South Korea.; Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Daejeon 34134, South Korea. EM hyounlim@cnu.ac.kr; jsmoon@kribb.re.kr FU "Cooperative Research Program for Agriculture Science & Technology Development" Rural Development Administration, Republic of Korea [PJ01007702] FX This research was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01007702)" Rural Development Administration, Republic of Korea. NR 33 TC 0 Z9 0 U1 15 U2 15 PU KOREAN SOC PLANT PATHOLOGY PI SUWON PA NATIONAL INST AGRICULTURAL SCIENCE & TECHNOLOGY, PLANT PATHOLOGY DIVISION, SUWON, 441-707, SOUTH KOREA SN 1598-2254 EI 2093-9280 J9 PLANT PATHOLOGY J JI Plant Pathol. J. PD OCT PY 2016 VL 32 IS 5 BP 441 EP 451 DI 10.5423/PPJ.OA.04.2016.0104 PG 11 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA DY1RA UT WOS:000384870900008 PM 27721694 ER PT J AU Cosme, S Cuevas, HE Zhang, D Oleksyk, TK Irish, BM AF Cosme, S. Cuevas, H. E. Zhang, D. Oleksyk, T. K. Irish, B. M. TI Genetic diversity of naturalized cacao (Theobroma cacao L.) in Puerto Rico SO TREE GENETICS & GENOMES LA English DT Article DE Chocolate; Germplasm; Cocoa; Tropics; STRUCTURE; SNP ID POPULATION-STRUCTURE; MORPHOLOGICAL VARIATION; IDENTIFICATION; DESCRIPTORS; COLLECTION; COMPONENTS; GERMPLASM; TRINIDAD; PROGRAM; MARKERS AB Identification of genetically diverse cacao with disease resistance, high productivity, and desirable organoleptic traits is vitally important to the agricultural crop's long-term sustainability. Environmental changes, pests, and diseases as well as nation's sovereign property rights have led to a decrease in accessibility and exchange of germplasm of interest. Having been introduced during colonial times, naturalized cacao in Puerto Rico could serve as an unexplored source of genetic diversity in improvement programs. An island-wide survey was carried out to identify naturalized trees and to determine their genetic associations to reference cacao accessions. Samples were genotyped with Expressed Sequence Tag-derived single nucleotide polymorphism (SNP) markers. Principal coordinate, cluster, and population structure analysis using the genotype data for both local and reference samples assigned individuals into five distinct genetic backgrounds: Criollo, Trinitario, Amelonado, Upper Amazon Forastero (UAF), and Nacional. Puerto Rican cacao fit into four (Criollo, Trinitario, Amelonado and UAF) of the five genetic backgrounds, being mainly composed of individuals of Criollo ancestry. Based on historical evidence, cacao of Criollo background was probably brought to Puerto Rico from Venezuela and/or Central America during colonial times. Trinitario, Amelonado, and UAF genetic backgrounds are most likely products of more modern introductions. Genotyping cacao in Puerto Rico provides information on the history and possible origin of the naturalized trees on the island. In addition, the assessment has allowed the targeting of material for incorporation and long-term conservation filling gaps in the existing collection and providing new germplasm to be evaluated for agronomic performance. C1 [Cosme, S.; Cuevas, H. E.; Irish, B. M.] ARS, USDA, Trop Agr Res Stn, 2200 Pedro Albizu Campos Ave, Mayaguez, PR 00680 USA. [Cosme, S.; Oleksyk, T. K.] Univ Puerto Rico, Dept Biol, Carr 108,Barrio Miradero Km 1-3, Mayaguez, PR USA. [Zhang, D.] ARS, USDA, Sustainable Perennial Crops Lab, BARC West, 1300 Baltimore Ave,Bldg 001,Rm 223, Beltsville, MD USA. RP Irish, BM (reprint author), ARS, USDA, Trop Agr Res Stn, 2200 Pedro Albizu Campos Ave, Mayaguez, PR 00680 USA. EM Brian.Irish@ars.usda.gov FU Chocolove Inc., a Boulder FX The authors thank Chocolove Inc., a Boulder, Colorado-based Chocolate company, for providing funding to support research conducted and for continued efforts in cacao conservation and use. In addition, the authors thank Drs. David Kuhn, Timothy Porch, and Chris Richards for their editorial comments. Lastly, authors thank the general public and all of the farmers (especially Mr. Juan Echevarria) who were instrumental in identifying cacao trees thought out the island and helped secure leaf and pod samples. NR 57 TC 0 Z9 0 U1 13 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD OCT PY 2016 VL 12 IS 5 AR 88 DI 10.1007/s11295-016-1045-4 PG 13 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA DY5MG UT WOS:000385143300006 ER PT J AU Stewart, JF Will, RE Crane, BS Nelson, CD AF Stewart, John F. Will, Rodney E. Crane, Barbara S. Nelson, C. Dana TI The genetics of shortleaf pine (Pinus echinata mill.) with implications for restoration and management SO TREE GENETICS & GENOMES LA English DT Review DE Shortleaf pine; Genetics; Conservation; Silviculture ID LOBLOLLY-PINE; TAEDA L.; HYBRIDS; POPULATIONS; HYBRIDIZATION; GENOME; MAP; DNA; INTROGRESSION; INHERITANCE AB Shortleaf pine (Pinus echinata Mill.) is an important commercial timber resource and forest ecosystem component in the southeastern USA. The species occurs in mainly drier sites as an early-to mid-successional species, is fire-adapted, and it plays an important role in the fire ecology of the region. However, shortleaf pine genetics are not well-studied, especially in this era of molecular genetics and genomics. Most genetics research about the species has focused on provenance testing. Generally, shortleaf pine performs well in colder areas, when compared to loblolly pine (Pinus taeda L.), a close relative, which is faster growing and the most common plantation species in the region. Though not as advanced in genetic improvement as loblolly pine, tree breeders have improved shortleaf pine in one to two generations of selection, and diverse, genetically improved shortleaf pine materials are available to foresters and landowners throughout the southeastern USA. Researchers have also studied the genetic variation of shortleaf pine using various molecular markers and have found that shortleaf pine is generally a prolific outcrosser, a trait it shares with other non-isolated members of the family Pinaceae. In recent years, however, it has shared less genetic material across long ranges, probably because of habitat fragmentation. Various anthropogenic factors also affect shortleaf pine's future, as recent studies show that shortleaf pine introgression with loblolly pine puts the species- and the resiliency of southeastern forests-at risk. Importantly, fire exclusion is a likely cause of the increase in introgression. Herein, we provide further details and up-to-date genetic information and resources for foresters and ecologists interested in the restoration and management of shortleaf pine. C1 [Stewart, John F.] Oklahoma State Univ, Dept Integrat Biol, Stillwater, OK 74078 USA. [Will, Rodney E.] Oklahoma State Univ, Dept Nat Resources Ecol & Management, Stillwater, OK 74078 USA. [Crane, Barbara S.] US Forest Serv, Southern Reg Off, USDA, Atlanta, GA USA. [Nelson, C. Dana] US Forest Serv, Southern Inst Forest Genet, Southern Res Stn, USDA, Saucier, MS USA. [Nelson, C. Dana] Univ Kentucky, Dept Forestry, Forest Hlth Res & Educ Ctr, Lexington, KY 40546 USA. RP Stewart, JF (reprint author), Oklahoma State Univ, Dept Integrat Biol, Stillwater, OK 74078 USA. EM john.stewart@okstate.edu RI Will, Rodney/G-8111-2011 NR 93 TC 0 Z9 0 U1 14 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD OCT PY 2016 VL 12 IS 5 AR 98 DI 10.1007/s11295-016-1052-5 PG 15 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA DY5MG UT WOS:000385143300016 ER PT J AU Jordan, N Schut, M Graham, S Barney, JN Childs, DZ Christensen, S Cousens, RD Davis, AS Eizenberg, H Ervin, DE Fernandez-Quintanilla, C Harrison, LJ Harsch, MA Heijting, S Liebman, M Loddo, D Mirsky, SB Riemens, M Neve, P Peltzer, DA Renton, M Williams, M Recasens, J Sonderskov, M AF Jordan, N. Schut, M. Graham, S. Barney, J. N. Childs, D. Z. Christensen, S. Cousens, R. D. Davis, A. S. Eizenberg, H. Ervin, D. E. Fernandez-Quintanilla, C. Harrison, L. J. Harsch, M. A. Heijting, S. Liebman, M. Loddo, D. Mirsky, S. B. Riemens, M. Neve, P. Peltzer, D. A. Renton, M. Williams, M. Recasens, J. Sonderskov, M. TI Transdisciplinary weed research: new leverage on challenging weed problems? SO WEED RESEARCH LA English DT Article DE agroecosystem processes; systems research; ecosystem services; crop protection; interdisciplinary research; multistakeholder processes ID AGRICULTURAL INNOVATION SYSTEMS; RAAIS RAPID APPRAISAL; SUSTAINABLE INTENSIFICATION; INTEGRATED ANALYSIS; ECOSYSTEM SERVICES; PEST-MANAGEMENT; PARASITIC WEEDS; SCIENCE; GOVERNANCE; RICE AB Transdisciplinary weed research (TWR) is a promising path to more effective management of challenging weed problems. We define TWR as an integrated process of inquiry and action that addresses complex weed problems in the context of broader efforts to improve economic, environmental and social aspects of ecosystem sustainability. TWR seeks to integrate scholarly and practical knowledge across many stakeholder groups (e.g. scientists, private sector, farmers and extension officers) and levels (e.g. local, regional and landscape). Furthermore, TWR features democratic and iterative processes of decision-making and collective action that aims to align the interests, viewpoints and agendas of a wide range of stakeholders. The fundamental rationale for TWR is that many challenging weed problems (e.g. herbicide resistance or extensive plant invasions in natural areas) are better addressed systemically, as a part of broad-based efforts to advance ecosystem sustainability, rather than as isolated problems. Addressing challenging weed problems systemically can offer important new leverage on such problems, by creating new opportunities to manage their root causes and by improving complementarity between weed management and other activities. While promising, this approach is complicated by the multidimensional, multilevel, diversely defined and unpredictable nature of ecosystem sustainability. In practice, TWR can be undertaken as a cyclic process of (i) initial problem formulation, (ii) 'broadening' of the problem formulation and recruitment of stakeholder participants, (iii) deliberation, negotiation and design of an action agenda for systemic change, (iv) implementation action, (v) monitoring and assessment of outcomes and (vi) reformulation of the problem situation and renegotiation of further actions. Notably, 'purposive' disciplines (design, humanities and arts) have central, critical and recurrent roles in this process, as do integrative analyses of relevant multidimensional and multilevel factors, via multiple natural and social science disciplines. We exemplify this process in prospect and retrospect. Importantly TWR is not a replacement for current weed research; rather, the intent is to powerfully leverage current efforts. C1 [Jordan, N.] Univ Minnesota, Agron & Plant Genet Dept, St Paul, MN 55108 USA. [Schut, M.] Wageningen Univ, Knowledge Technol & Innovat, NL-6700 EW Wageningen, Netherlands. [Schut, M.] Int Inst Trop Agr, POB 1269, Kigali, Rwanda. [Graham, S.] Univ New South Wales, Sch Social Sci, Sydney, NSW, Australia. [Barney, J. N.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA USA. [Childs, D. Z.] Univ Sheffield, Dept Anim & Plant Sci, Sheffield, S Yorkshire, England. [Christensen, S.] Univ Copenhagen, Dept Plant & Environm Sci, Frederiksberg C, Denmark. [Cousens, R. D.] Univ Melbourne, Sch BioSci, Melbourne, Vic, Australia. [Davis, A. S.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. [Eizenberg, H.] Agr Res Org, Dept Plant Pathol & Weed Res, Newe Yaar Res Ctr, Ramat Yishay, Israel. [Ervin, D. E.] Portland State Univ, Inst Sustainable Solut, Portland, OR 97207 USA. [Fernandez-Quintanilla, C.] CSIC Inst Agr Sci, Madrid, Spain. [Harrison, L. J.] Univ York, Dept Environm, York, N Yorkshire, England. [Harsch, M. A.] Univ Washington, Dept Biol, Seattle, WA 98195 USA. [Heijting, S.] Univ Wageningen & Res Ctr, Agrosyst Res, Wageningen, Netherlands. [Liebman, M.] Iowa State Univ, Dept Agron, Ames, IA USA. [Loddo, D.] CNR, Inst Agroenvironm & Forest Biol, Legnaro, Italy. [Mirsky, S. B.] ARS, Sustainable Agr Syst Lab, USDA, Beltsville, MD USA. [Riemens, M.] Univ Wageningen & Res Ctr, Appl Plant Res, Lelystad, Netherlands. [Neve, P.] Rothamsted Res, Agroecol, Harpenden, Herts, England. [Peltzer, D. A.] Landcare Res, Ecosyst & Global Change, Lincoln, New Zealand. [Renton, M.] Univ Western Australia, Sch Plant Biol, Crawley, WA, Australia. [Williams, M.] Michael Williams & Associates Pty Ltd, Sydney, NSW, Australia. [Recasens, J.] Univ Lleida Agrotecnio, ETSEA, Dept Hort Bot & Landscaping, Lleida, Spain. [Sonderskov, M.] Aarhus Univ, Agroecol, Slagelse, Denmark. RP Schut, M (reprint author), Wageningen Univ, Knowledge Technol & Innovat, NL-6700 EW Wageningen, Netherlands.; Schut, M (reprint author), Int Inst Trop Agr, POB 1269, Kigali, Rwanda. EM m.schut@cgiar.org RI Peltzer, Duane/A-9463-2009; Recasens, Jordi/B-3970-2011; Neve, Paul/P-8726-2015; Christensen, Svend/B-9831-2014; OI Peltzer, Duane/0000-0001-7724-3738; Recasens, Jordi/0000-0002-8799-9958; Neve, Paul/0000-0002-3136-5286; Christensen, Svend/0000-0002-1112-1954; Schut, Marc/0000-0002-3361-4581; Harrison, Laura/0000-0003-3923-7083; Barney, Jacob/0000-0003-2949-5003 NR 79 TC 0 Z9 0 U1 17 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD OCT PY 2016 VL 56 IS 5 BP 345 EP 358 DI 10.1111/wre.12219 PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY3VU UT WOS:000385024600003 ER PT J AU Gonzalez-Andujar, JL Francisco-Fernandez, M Cao, R Reyes, M Urbano, JM Forcella, F Bastida, F AF Gonzalez-Andujar, J. L. Francisco-Fernandez, M. Cao, R. Reyes, M. Urbano, J. M. Forcella, F. Bastida, F. TI A comparative study between nonlinear regression and nonparametric approaches for modelling Phalaris paradoxa seedling emergence SO WEED RESEARCH LA English DT Article DE awned canary grass; hood canary grass; hydrothermal time; weed emergence model; distribution functions; Weibull; Gompertz; Logistic ID PREDICTING WEED EMERGENCE; RAGWEED AMBROSIA-TRIFIDA; AVENA-FATUA; BROMUS-DIANDRUS; WINTER CEREALS; GERMINATION; DORMANCY; MAIZE; CROPS; TIME AB Parametric nonlinear regression (PNR) models are used widely to fit weed seedling emergence patterns to soil microclimatic indices. However, such approximation has been questioned, mainly due to several statistical limitations. Alternatively, nonparametric approaches can be used to overcome the problems presented by PNR models. Here, we used an emergence data set of Phalaris paradoxa to compare both approaches. Mean squared error and correlation results indicated higher accuracy for the descriptive ability but similar poor performance for predictive ability of the nonparametric approach in comparison with the PNR approach. These results suggest that our nonparametric cumulative distribution function approach is a valuable alternative to the classical parametric nonlinear regression models to describe complex emergence patterns for P. paradoxa, but not to predict them. C1 [Gonzalez-Andujar, J. L.] Inst Agr Sostenible CSIC, Apartado 4084, Cordoba 14080, Spain. [Francisco-Fernandez, M.; Cao, R.; Reyes, M.] Univ A Coruna, Fac Informat, Dept Matemat, La Coruna, Spain. [Urbano, J. M.] Univ Seville, ETSIA, Seville, Spain. [Forcella, F.] ARS, North Cent Soil Conservat Res Lab, USDA, Morris, MN USA. [Bastida, F.] Univ Huelva, ETSI, Dept Ciencias Agroforestales, Huelva, Spain. RP Gonzalez-Andujar, JL (reprint author), Inst Agr Sostenible CSIC, Apartado 4084, Cordoba 14080, Spain. EM andujar@cica.es RI GONZALEZ-ANDUJAR, JOSE /G-5671-2013 OI GONZALEZ-ANDUJAR, JOSE /0000-0003-2356-4098 FU Spanish Ministry of Science and Innovation [MTM2011-22392, MTM2014-52876-R]; FEDER (European Regional Development Fund); Spanish Ministry of Economy and Competitiveness [AGL2012-33736] FX This research has been partially supported by the Spanish Ministry of Science and Innovation Grant MTM2011-22392 and MTM2014-52876-R for the second, third and fourth authors and by FEDER (European Regional Development Fund) and the Spanish Ministry of Economy and Competitiveness Grant AGL2012-33736 for the first and last authors. NR 34 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD OCT PY 2016 VL 56 IS 5 BP 367 EP 376 DI 10.1111/wre.12216 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY3VU UT WOS:000385024600005 ER PT J AU Smith, D Zamora, DS Lazarus, W AF Smith, David Zamora, Diomides S. Lazarus, William TI The economics of planting and producing biomass from willow (Salix spp.) living snow fences SO AGROFORESTRY SYSTEMS LA English DT Article DE Windbreak; Enterprise budgets; Transportation; Snow ID MINNESOTA; IRELAND; YIELDS; CROPS AB Blowing snow adversely affects winter transportation by reducing driver's visibility, creating icy roads, and depositing snow drifts in the travel lane. Blowing snow is prevalent in snowy and windy climates where landscapes lack sufficient vegetation to trap snow. Maintaining safe driving conditions on roads with blowing snow can be a costly challenge for transportation agencies. Living snow fences (LSFs) are semi-permanent living structures that can reduce blowing and drifting snow and offer environmental benefits, such as carbon sequestration and wildlife habitat. Recently, shrub willow cultivars (Salix spp.) have been evaluated as a potential LSF due to the relative ease of planting, reduction in plant material costs, fast growth, and ability to establish well given proper site preparation, planting techniques and maintenance. To evaluate the potential of willow for LSFs this study analyzes the costs of planting and establishing a willow LSF and the viability of harvesting biomass. This study finds that the costs of planting and establishing a willow LSF is $8.11 m(-1) for a two row snow fence. Biomass harvest is prohibitively expensive for the typical willow LSF due to the small scale of production. However, corridor length willow LSFs, in which planting and establishment costs are defrayed due to payments for the transportation benefits, can produce biomass at a cost of $30 dry-Mg-1.. C1 [Smith, David] Econ Res Serv, USDA, 355 E St SW, Washington, DC 20024 USA. [Zamora, Diomides S.] Univ Minnesota Extens, 1530 Cleveland Ave N, St Paul, MN 55108 USA. [Lazarus, William] Univ Minnesota, Appl Econ, 1994 Buford Ave, St Paul, MN 55108 USA. RP Zamora, DS (reprint author), Univ Minnesota Extens, 1530 Cleveland Ave N, St Paul, MN 55108 USA. EM zamor015@umn.edu NR 24 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD OCT PY 2016 VL 90 IS 5 SI SI BP 737 EP 746 DI 10.1007/s10457-016-9935-x PG 10 WC Agronomy; Forestry SC Agriculture; Forestry GA DX7JK UT WOS:000384562700004 ER PT J AU Ziegler, J Easter, M Swan, A Brandle, J Ballesteros, W Domke, G Chambers, A Eve, M Paustian, K AF Ziegler, Justin Easter, Mark Swan, Amy Brandle, James Ballesteros, William Domke, Grant Chambers, Adam Eve, Marlen Paustian, Keith TI A model for estimating windbreak carbon within COMET-Farm (TM) SO AGROFORESTRY SYSTEMS LA English DT Article DE Greenhouse gas accounting; COMET-Farm (TM); Windbreak; Carbon sequestration ID INCREMENT MODEL; PONDEROSA PINE; HEIGHT; AGE; TREES; FIR AB Agroforestry as a land management practice presents a method for partially offsetting greenhouse gas emissions from agricultural land. Of all agroforestry practices in the United States, windbreaks in particular are used throughout the United States providing a useful starting point for deriving a modelling system which could quantify the amount of carbon sequestered on U.S. agricultural land and provide for broad usability. We present our first approximation to this end by presenting a model that estimates current and future stocks within multiple carbon pools of windbreak systems such as live trees, the O horizon, downed woody debris and standing dead trees. In this article, we describe each modelled process driving carbon fluxes within carbon pools including novel windbreak tree growth and mortality models. Our model is generalized by region and species group allowing us to run scenarios for any common tree species in any location within the contiguous United States. Integrated into the agricultural greenhouse gas accounting tool, COMET-Farm (TM), the windbreak component gives landowners and land managers power to view agroforestry systems in the same context as agricultural operations and provides an alternative to intensive biomass inventories. C1 [Ziegler, Justin; Easter, Mark; Swan, Amy] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Brandle, James] Univ Nebraska, Sch Nat Resources, Lincoln, NE USA. [Ballesteros, William] Univ Narino, Fac Crop Sci, Pasto, Narino, Colombia. [Domke, Grant] US Forest Serv, Northern Res Stn, USDA, St Paul, MN USA. [Chambers, Adam] Nat Resources Conservat Serv, West Natl Technol Support Ctr, USDA, Portland, OR USA. [Eve, Marlen] ARS, USDA, Washington, DC USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Paustian, Keith] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Ziegler, J (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. EM Justin.Ziegler@colostate.edu NR 36 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD OCT PY 2016 VL 90 IS 5 SI SI BP 875 EP 887 DI 10.1007/s10457-016-9977-0 PG 13 WC Agronomy; Forestry SC Agriculture; Forestry GA DX7JK UT WOS:000384562700014 ER PT J AU Possu, WB Brandle, JR Domke, GM Schoeneberger, M Blankenship, E AF Ballesteros Possu, William Brandle, James R. Domke, Grant M. Schoeneberger, Michele Blankenship, Erin TI Estimating carbon storage in windbreak trees on US agricultural lands SO AGROFORESTRY SYSTEMS LA English DT Article DE Climate change; Agroforestry; Allometric models; Tree biomass; Carbon storage; Open-grown trees ID ALLOMETRIC RELATIONSHIPS; ABOVEGROUND BIOMASS; LODGEPOLE PINE; FORESTS; AGROFORESTRY; EQUATIONS; STANDS; STOCKS; SITES AB Assessing carbon (C) capture and storage potential by the agroforestry practice of windbreaks has been limited. This is due, in part, to a lack of suitable data and associated models for estimating tree biomass and C for species growing under more open-grown conditions such as windbreaks in the Central Plains region of the United States (U.S.). We evaluated 15 allometric models using destructively sampled Pinus ponderosa (Lawson & C. Lawson) data from field windbreaks in Nebraska and Montana. Several goodness-of-fit metrics were used to select the optimal model. The Jenkins' et al. model was then used to estimate biomass for 16 tree species in windbreaks projected over a 50 year time horizon in nine continental U.S. regions. Carbon storage potential in the windbreak scenarios ranged from 1.07 +/- 0.21 to 3.84 +/- 0.04 Mg C ha(-1) year(-1) for conifer species and from 0.99 +/- 0.16 to 13.6 +/- 7.72 Mg C ha(-1) year(-1) for broadleaved deciduous species during the 50 year period. Estimated mean C storage potentials across species and regions were 2.45 +/- 0.42 and 4.39 +/- 1.74 Mg C ha(-1) year(-1) for conifer and broadleaved deciduous species, respectively. Such information enhances our capacity to better predict the C sequestration potential of windbreaks associated with whole farm/ranch operations in the U.S. C1 [Ballesteros Possu, William] Univ Narino, Fac Agr Sci, San Juan De Pasto, Narino, Colombia. [Brandle, James R.] Univ Nebraska, Sch Nat Resources, 3310 Holdrege St, Lincoln, NE 68583 USA. [Domke, Grant M.] US Forest Serv, USDA, 1992 Folwell Ave, St Paul, MN 55108 USA. [Schoeneberger, Michele] Forest Serv, Natl Agroforestry Ctr, USDA, East Campus UNL, Lincoln, NE 68583 USA. [Blankenship, Erin] Univ Nebraska, Dept Stat, 340 Hardin Hall, Lincoln, NE 68585 USA. RP Possu, WB (reprint author), Univ Narino, Fac Agr Sci, San Juan De Pasto, Narino, Colombia. EM wballesterosp@gmail.com OI Ballesteros, William/0000-0001-6633-2460; Domke, Grant/0000-0003-0485-0355 NR 71 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD OCT PY 2016 VL 90 IS 5 SI SI BP 889 EP 904 DI 10.1007/s10457-016-9896-0 PG 16 WC Agronomy; Forestry SC Agriculture; Forestry GA DX7JK UT WOS:000384562700015 ER PT J AU Runyon, JB Birdsall, JL AF Runyon, Justin B. Birdsall, Jennifer L. TI Costs of induced defenses for the invasive plant houndstongue (Cynoglossum officinale L.) and the potential importance for weed biocontrol SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Induced plant defenses; Costs; Biological control; Efficacy; Invasive plant; Herbivore; Cynoglossum officinale ID BIOLOGICAL-CONTROL; ARABIDOPSIS-THALIANA; NICOTIANA-ATTENUATA; INDUCED RESPONSES; FITNESS COSTS; TRICHOME PRODUCTION; METHYL JASMONATE; GALL INSECTS; COMPETITION; HERBIVORES AB Inducible plant defenses-those produced in response to herbivore feeding-are thought to have evolved as a cost-saving tactic that allows plants to enact defenses only when needed. The costs of defense can be significant, and loss of plant fitness due to commitment of resources to induced defenses could affect plant populations and play a role in determining the success or failure of weed biocontrol. We used methyl jasmonate (MeJA) to experimentally induce defenses without herbivores in invasive houndstongue plants (Cynoglossum officinale L.) in the field and measured resulting growth and fitness (plant size, seed number, and seed weight). MeJA-treated plants emitted large amounts of plant volatiles and produced leaves with twice as many trichomes as untreated plants. Plants with activated defenses had fewer leaves, were smaller, and produced nutlets that weighed less than plants not investing in defenses. These data indicate that herbivore-induced defenses are costly for houndstongue plants in their invaded range and represent significant indirect costs of herbivory beyond direct feeding damage (e.g., loss of photosynthetic tissue). Notably, the magnitude of defenses elicited upon feeding varies greatly by herbivore species and a better understanding of the costs of defense could help us predict which potential biocontrol herbivores are most likely to be effective. C1 [Runyon, Justin B.; Birdsall, Jennifer L.] US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, USDA, Bozeman, MT 59718 USA. RP Runyon, JB (reprint author), US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, USDA, Bozeman, MT 59718 USA. EM jrunyon@fs.fed.us FU PECASE (President's Early Career Award in Science and Engineering); Rocky Mountain Research Station, USDA Forest Service FX We thank Thomas O'Neil and Casey M. Delphia for assistance in the field and lab. Casey Delphia, Deb Finch, and three anonymous reviewers provided helpful comments on the manuscript. Reggie Clark and John Councilman (Gallatin National Forest) generously helped find and allowed access to the field sites. This project was supported by funding from PECASE (President's Early Career Award in Science and Engineering) to Justin Runyon and the Rocky Mountain Research Station, USDA Forest Service. NR 57 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD OCT PY 2016 VL 10 IS 5 BP 383 EP 391 DI 10.1007/s11829-016-9449-5 PG 9 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA DW4DE UT WOS:000383591700003 ER PT J AU Zhang, XY Wan, SW Hao, JJ Hu, JG Yang, T Zong, XX AF Zhang, Xiaoyan Wan, Shuwei Hao, Junjie Hu, Jinguo Yang, Tao Zong, Xuxiao TI Large-scale evaluation of pea (Pisum sativum L.) germplasm for cold tolerance in the field during winter in Qingdao SO CROP JOURNAL LA English DT Article DE Pisum sativum L.; Cold tolerance; Germplasm evaluation; Open-field experiment ID SEASON FOOD LEGUMES; FREEZING RESISTANCE; FABA BEANS; SCREENING TECHNIQUES; ABIOTIC STRESSES; LENTIL; HARDINESS; POPULATIONS AB As a cool-season crop, pea (Pisum sativum L.) can tolerate frost at the vegetative stage but experiences yield loss when freezing stress occurs at the reproductive stage. Cold-tolerance improvement of pea varieties is important for stable yield and expansion of the winter pea planting area. Under natural low-temperature conditions during winter in Qingdao, Shandong, China, we evaluated the cold tolerance of 3672 pea germplasm accessions in the field and categorized them as displaying high resistance (214), moderate resistance (835), or susceptibility (2623). The highly and moderately resistant genotypes were validated in the following year. We found that genotypes from the winter production region showed higher cold tolerance than genotypes from the spring production region. The accessions identified as having high levels of cold tolerance are recommended as potential genetic resources in cold-tolerance breeding of pea. (C) 2016 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Zhang, Xiaoyan; Wan, Shuwei; Hao, Junjie] Qingdao Acad Agr Sci, Qingdao 266100, Peoples R China. [Yang, Tao; Zong, Xuxiao] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China. [Hu, Jinguo] Washington State Univ, Western Reg Plant Intro Stn, ARS, USDA, Pullman, WA 99164 USA. RP Zong, XX (reprint author), Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China. EM zongxuxiao@caas.cn NR 29 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD OCT PY 2016 VL 4 IS 5 BP 377 EP 383 DI 10.1016/j.cj.2016.06.016 PG 7 WC Agronomy SC Agriculture GA DX7BG UT WOS:000384540300006 ER PT J AU Sen Gupta, D Cheng, P Sablok, G Thavarajah, D Thavarajah, P Coyne, CJ Kumar, S Baum, M McGee, RJ AF Sen Gupta, Debjyoti Cheng, Peng Sablok, Gaurav Thavarajah, Dil Thavarajah, Pushparajah Coyne, Clarice J. Kumar, Shiv Baum, Michael McGee, Rebecca J. TI Development of a panel of unigene-derived polymorphic EST-SSR markers in lentil using public database information SO CROP JOURNAL LA English DT Article DE Lens culinaris; EST-SSRs; Functional annotation; Unigene sequences; EST database; Genetic resources ID LENS-CULINARIS MEDIK.; PISUM-SATIVUM L.; MICROSATELLITE MARKERS; GENETIC DIVERSITY; MEDICAGO-TRUNCATULA; WINTER HARDINESS; SNP DISCOVERY; REGISTRATION; TRANSCRIPTOME; IDENTIFICATION AB Lentil (Lens culinaris Medik.), a diploid (2n = 14) with a genome size greater than 4000 Mbp, is an important cool season food legume grown worldwide. The availability of genomic resources is limited in this crop species. The objective of this study was to develop polymorphic markers in lentil using publicly available curated expressed sequence tag information (ESTs). In this study, 9513 ESTs were downloaded from the National Center for Biotechnology Information (NCBI) database to develop unigene-based simple sequence repeat (SSR) markers. The ESTs were assembled into 4053 unigenes and then analyzed to identify 374 SSRs using the MISA microsatellite identification tool. Among the 374 SSRs, 26 compound SSRs were observed. Primer pairs for these SSRs were designed using Primer3 version 1.14. To classify the functional annotation of ESTs and EST-SSRs, BLASTx searches (using E-value 1 x 10(-5)) against the public UniProt (http://www.uniprot.org/) and NCBI (http://www.ncbi.nlh.nih.gov/) databases were performed. Further functional annotation was performed using PLAZA (version 3.0) comparative genomics and GO annotation was summarized using the Plant GO slim category. Among the synthesized 312 primers, 219 successfully amplified Lens DNA. A diverse panel of 24 Lens genotypes was used to identify polymorphic markers. A polymorphic set of 57 markers successfully discriminated the test genotypes. This set of polymorphicmarkers with functional annotation data could be used as molecular tools in lentil breeding. Production and hosting by Elsevier B.V. on behalf of Crop Science Society of China and Institute of Crop Science, CAAS. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Sen Gupta, Debjyoti] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Sen Gupta, Debjyoti] Indian Inst Pulses Res, ICAR ARS, Kanpur 208024, Uttar Pradesh, India. [Cheng, Peng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Sablok, Gaurav] Fdn Edmund Mach, Res & Innovat Ctr, Dept Biodivers & Mol Ecol, Via E Mach 1, I-38010 San Michele All Adige, Trento, Italy. [Thavarajah, Dil] Clemson Univ, Agr & Environm Sci, Clemson, SC 29634 USA. [Thavarajah, Pushparajah] China Agr Univ, Int Coll Beijing, Beijing, Peoples R China. [Coyne, Clarice J.] Washington State Univ, Western Reg Plant Intro Stn, USDA ARS, Pullman, WA 99164 USA. [Kumar, Shiv] ICARDA, Rabat Inst, BIGM Program, Rabat, Morocco. [Baum, Michael] ICARDA, BIGM Program, Beirut, Lebanon. [McGee, Rebecca J.] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. RP McGee, RJ (reprint author), Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. EM rjmcgee@wsu.edu NR 43 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD OCT PY 2016 VL 4 IS 5 BP 425 EP 433 DI 10.1016/j.cj.2016.06.012 PG 9 WC Agronomy SC Agriculture GA DX7BG UT WOS:000384540300012 ER PT J AU Zhao, BL Zhang, JE Yan, WB Kang, XW Cheng, CG Ouyang, Y AF Zhao, Benliang Zhang, Jia-en Yan, Wenbin Kang, Xiaowu Cheng, Chaogang Ouyang, Ying TI Removal of cadmium from aqueous solution using waste shells of golden apple snail SO DESALINATION AND WATER TREATMENT LA English DT Article DE Cadmium removal; Precipitation; Golden apple snail shell; Waste water; Sorption isotherm ID HEAVY-METAL IONS; COAL FLY-ASH; POMACEA-CANALICULATA; SACCHAROMYCES-CEREVISIAE; ADSORPTION; BIOSORPTION; WATER; CD(II); PB(II); CU(II) AB Golden apple snail (GAS) is one of the 100 worst invasive alien species. With the application of molluscicides to kill and control the spreading of these snails, a large amount of dead GAS shells are remained in many farms. This study ascertained the characteristics and removal of cadmium (Cd) by the GAS shell (GASS) powders and the associate mechanisms. Results showed that when the concentration of shell powder increased from 0.05 to 10.00g/L, pH from 1.5 to 5.5, contact time from 2 to 100min, and temperature from 10 to 50 degrees C, the Cd removal efficiency from the solution increased gradually and reached an equilibrium condition. The Cd removal efficiency decreased as the initial Cd concentration increased from 5 to 500mg/L. The changes in the amount of Cd removal conformed to the Langmuir model with a capacity of 81.301mg/g. A thermodynamic analysis indicated that the Cd removal by the GASS powder was an endothermic and spontaneous process. Our study also revealed that the solution pH was affected greatly by the amount of GASS powder. The powder X-ray diffractometer (XRD) and scanning electron microscopy indicated that the CdCO3 precipitated on the surface of GAS shells. The Cd removal was related to the ion exchange and the micro-precipitation with a depletion of aragonite and calcite. This study suggests that shells of GAS could be used as an effective biomaterial for Cd removal from contaminated water. C1 [Zhao, Benliang; Zhang, Jia-en; Yan, Wenbin; Kang, Xiaowu; Cheng, Chaogang] South China Agr Univ, Inst Trop & Subtrop Ecol, Guangzhou 510642, Guangdong, Peoples R China. [Zhao, Benliang; Zhang, Jia-en] Guangdong Engn Res Ctr Modern Ecoagr & Circular A, Key Lab Agroecol & Rural Environm, Guangdong Regular Higher Educ Inst, Key Lab Ecol Agr,Minist Agr Peoples Republ China, Guangzhou 510642, Guangdong, Peoples R China. [Ouyang, Ying] US Forest Serv, USDA, 775 Stone Blvd,Thompson Hall,Room 309, Starkville, MS 39762 USA. RP Zhang, JE (reprint author), South China Agr Univ, Inst Trop & Subtrop Ecol, Guangzhou 510642, Guangdong, Peoples R China.; Zhang, JE (reprint author), Guangdong Engn Res Ctr Modern Ecoagr & Circular A, Key Lab Agroecol & Rural Environm, Guangdong Regular Higher Educ Inst, Key Lab Ecol Agr,Minist Agr Peoples Republ China, Guangzhou 510642, Guangdong, Peoples R China. EM blzhao@scau.edu.cn; jeanzh@scau.edu.cn; 229051655@qq.com; 281475493@qq.com; 812968527@qq.com; youyang@fs.fed.us FU National Natural Science Foundation of China [U1131006, 30770403, 30900187]; Training Project for High-level Talents of Guangdong Regular Higher Education Institutions; Science and Technology Program of Guangdong province, China [2015B090903077]; President Foundation of South China Agricultural University [2009K029]; China Scholarship Council FX This work is supported by the National Natural Science Foundation of China (Nos. U1131006, 30770403, and 30900187), Training Project for High-level Talents of Guangdong Regular Higher Education Institutions, Science and Technology Program of Guangdong province, China (No. 2015B090903077), the President Foundation of South China Agricultural University (No. 2009K029), and China Scholarship Council. NR 41 TC 0 Z9 0 U1 12 U2 12 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1944-3994 EI 1944-3986 J9 DESALIN WATER TREAT JI Desalin. Water Treat. PD OCT PY 2016 VL 57 IS 50 BP 23987 EP 24003 DI 10.1080/19443994.2016.1140078 PG 17 WC Engineering, Chemical; Water Resources SC Engineering; Water Resources GA DX8XM UT WOS:000384675100049 ER PT J AU Quesada-Pineda, H Wiedenbeck, J Bond, B AF Quesada-Pineda, Henry Wiedenbeck, Jan Bond, Brian TI Analysis of electricity consumption: a study in the wood products industry SO ENERGY EFFICIENCY LA English DT Article DE Energy usage; Wood products; Energy performance metrics ID US HARDWOOD INDUSTRY; ENERGY-CONSUMPTION; EFFICIENCY; SAWMILLS; PRICES AB This paper evaluates the effect of industry segment, year, and US region on electricity consumption per employee, per dollar sales, and per square foot of plant area for wood products industries. Data was extracted from the Industrial Assessment Center (IAC) database and imported into MS Excel. The extracted dataset was examined for outliers and abnormalities with outliers outside the quantile range 0.5-99.5 dropped from the analysis. A logarithmic transformation was applied to eliminate the skewness of the original data distributions. Correlation measurements indicated a moderate association between the response variables; therefore, a multivariate analysis of variance test was performed to measure the impact of the three factors: industry type, year, and region, simultaneously on all response variables. The results indicated some effect associated with all three factors on the three measures of electricity consumption. Subsequently, univariate ANOVA tests were conducted to determine the levels of the factors that were different. Most levels of industry type were associated with significantly different energy consumption, an expected result since some of the industries are more energy intensive than others. The industries in Standard Industry Code (SIC) 2493 (reconstituted wood products) are the groups with the highest electricity consumption with means of 38,096.28 kWh/employee, 0.86 kWh/sales, and 154.14 kWh/plant area while industries grouped in SIC 2451 (mobile homes) have the smallest consumption with means of 6811.01 kWh/employee, 0.05 kWh/sales, and 9.45 kWh/plant area. Interestingly, differences in regional consumption were found to be linked to the proportion of industry types by region. Data analysis also indicated differences in electricity consumption per employee for the factor year, but for the other response variables, no differences were found. These main results indicate that industries in the wood products sector have different electricity consumption rates depending on the type of manufacturing processes they use. Therefore, industries in this sector can use these comparisons and metrics to benchmark their electricity consumption as well to understand better how electricity costs might vary depending on the region they are located. C1 [Quesada-Pineda, Henry; Bond, Brian] Virginia Tech, 1650 Res Tech Dr, Blacksburg, VA 24061 USA. [Wiedenbeck, Jan] US Forest Serv, 241 Mercer Spring Rd, Princeton, WV 24740 USA. RP Quesada-Pineda, H (reprint author), Virginia Tech, 1650 Res Tech Dr, Blacksburg, VA 24061 USA. EM quesada@vt.edu FU US Forest Service [13-JV-11242301-080]; Department of Sustainable Biomaterials at Virginia Tech FX This work was funded through a cooperative agreement (13-JV-11242301-080) with the US Forest Service and The Department of Sustainable Biomaterials at Virginia Tech NR 30 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1570-646X EI 1570-6478 J9 ENERG EFFIC JI Energy Effic. PD OCT PY 2016 VL 9 IS 5 BP 1193 EP 1206 DI 10.1007/s12053-015-9417-4 PG 14 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels; Environmental Studies SC Science & Technology - Other Topics; Energy & Fuels; Environmental Sciences & Ecology GA DX7MU UT WOS:000384572600013 ER PT J AU Westfall, JA McRoberts, RE Radtke, PJ Weiskittel, AR AF Westfall, James A. McRoberts, Ronald E. Radtke, Philip J. Weiskittel, Aaron R. TI Effects of uncertainty in upper-stem diameter information on tree volume estimates SO EUROPEAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Error propagation; Measurement variability; Taper model; Quality assurance; Forest inventory ID NATIONAL FOREST INVENTORY; MODEL PREDICTIONS; TAPER EQUATION; UNITED-STATES AB Almost all relevant data in forestry databases arise from either field measurement or model prediction. In either case, these values have some amount of uncertainty that is often overlooked when doing analyses. In this study, the uncertainty associated with both measured and predicted data was quantified for upper-stem diameter at 5.27 m. This uncertainty was propagated through a tree taper model into predictions of individual-tree volume. The effects of uncertainty on individual-tree volume predictions and population estimates of total volume were assessed. Generally, when little or no systematic measurement deviation was present, less uncertainty was associated with field-measured diameters compared to model predictions. However, diameters predicted from a model were preferred when systematic deviations in field measurement exceeded approximately 0.2 cm. Comparisons of results obtained from an alternative taper model showed that more precise estimates of population totals might be obtained without upper-stem diameter information. Upper-stem diameter information increases the prediction accuracy of individual-tree volume, and thus, models using this information may be preferable in applications such as timber sales containing high-value trees. Due to the various factors that influence measurement and modeling uncertainty, foresters are encouraged to make similar evaluations in the context of their specific activities. C1 [Westfall, James A.] US Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. [McRoberts, Ronald E.] US Forest Serv, Northern Res Stn, St Paul, MN USA. [Radtke, Philip J.] Virginia Tech, Blacksburg, VA USA. [Weiskittel, Aaron R.] Univ Maine, Orono, ME USA. RP Westfall, JA (reprint author), US Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. EM jameswestfall@fs.fed.us; rmcroberts@fs.fed.us; pradtke@vt.edu; aaron.weiskittel@umit.maine.edu NR 28 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4669 EI 1612-4677 J9 EUR J FOREST RES JI Eur. J. For. Res. PD OCT PY 2016 VL 135 IS 5 BP 937 EP 947 DI 10.1007/s10342-016-0985-4 PG 11 WC Forestry SC Forestry GA DX7MM UT WOS:000384571700012 ER PT J AU Preuett, JA Collins, DJ Luster, DG Widmer, TL AF Preuett, Jason A. Collins, Daniel J. Luster, Douglas G. Widmer, Timothy L. TI The effect of salinity on the survival, growth, sporulation and infection of Phytophthora ramorum SO FUNGAL ECOLOGY LA English DT Article DE Brackish water; Epidemiology; Phytophthora ramorum; Ramorum blight; Salinity ID SOIL-SALINITY; WATER MOLDS; ROOT-ROT; NURSERIES; PH; HALOPHYTOPHTHORA; TEMPERATURE; PARASITICA; CINNAMOMI; ZOOSPORES AB Phytophthora ramorum has been found in waterways outside infested nurseries, but little is known about its behavior in water. This study examined the effect of salinity on survival, growth, sporulation, and infection. P. ramorum survival and growth was negatively correlated with salt concentration (range of 0 - 45 g l(-1)), but showed a level of tolerance even at 45 g l(-1). No sporangia were observed in cultures with higher than 20 g l(-1) of salt and zoospores were not released from sporangia above 14 g l(-1). Water sources with different salinity were used to understand the environment where P. ramorum can survive and infect host material. Water from natural bodies and water amended with different salt concentrations were added to P. ramorum-infested sand and baited with rhododendron leaf disks. Infection decreased with increasing salt concentration and increased with higher initial concentration of P. ramorum. This research helps to better understand the effects of water quality on survival and infectivity of P. ramorum, expanding the potential survey range. Published by Elsevier Ltd. C1 [Preuett, Jason A.; Collins, Daniel J.] Southern Univ & A&M Coll, Urban Forestry Program, Baton Rouge, LA 70813 USA. [Luster, Douglas G.; Widmer, Timothy L.] ARS, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. [Preuett, Jason A.] Eagle Environm Serv Inc, Baton Rouge, LA 70809 USA. [Collins, Daniel J.] Alcorn State Univ, Dept Agr, Alcorn State, MS 39096 USA. RP Widmer, TL (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM tim.widmer@ars.usda.gov FU United States Department of Agriculture-National Institute of Food and Agriculture 1890 Capacity Building Grant [2010-38821-21539] FX The authors wish to thank Ms. Bridget Cantwell for her technical assistance. This research was partially funded by the United States Department of Agriculture-National Institute of Food and Agriculture 1890 Capacity Building Grant 2010-38821-21539. NR 39 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 EI 1878-0083 J9 FUNGAL ECOL JI Fungal Ecol. PD OCT PY 2016 VL 23 BP 123 EP 130 DI 10.1016/j.funeco.2016.08.002 PG 8 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA DY0KY UT WOS:000384786900014 ER PT J AU Kelly, A Proctor, RH Belzile, F Chulze, SN Clear, RM Cowger, C Elmer, W Lee, T Obanor, F Waalwijk, C Ward, TJ AF Kelly, Amy Proctor, Robert H. Belzile, Francois Chulze, Sofia N. Clear, Randall M. Cowger, Christina Elmer, Wade Lee, Theresa Obanor, Friday Waalwijk, Cees Ward, Todd J. TI The geographic distribution and complex evolutionary history of the NX-2 trichothecene chemotype from Fusarium graminearum SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Fusarium head blight; Trichothecene; NX-2; TRI1; Phylogenetics; Molecular evolution ID MIDWESTERN UNITED-STATES; CODON-SUBSTITUTION MODELS; HEAD BLIGHT PATHOGENS; AMINO-ACID SITES; SPECIES COMPLEX; PHYLOGENETIC ANALYSIS; POPULATION SUBDIVISION; NUCLEOTIDE-SEQUENCES; REGIONAL DIFFERENCES; MAXIMUM-LIKELIHOOD AB Fusarium graminearum and 21 related species comprising the F. sambucinum species complex lineage 1 (FSAMSC-1) are the most important Fusarium Head Blight pathogens of cereal crops world-wide. FSAMSC-1 species typically produce type B trichothecenes. However, some F. graminearum strains were recently found to produce a novel type A trichothecene (NX-2) resulting from functional variation in the trichothecene biosynthetic enzyme Tril. We used a PCR-RFLP assay targeting the TRI1 gene to identify the NX-2 allele among a global collection of 2515 F. graminearum. NX-2 isolates were only found in southern Canada and the northern U.S., where they were observed at low frequency (1.8%), but over a broader geographic range and set of cereal hosts than previously recognized. Phylogenetic analyses of TRI1 and adjacent genes produced gene trees that were incongruent with the history of species divergence within FSAMSC-1, indicating trans-species evolution of ancestral polymorphism. In addition, placement of NX-2 strains in the TRI1 gene tree was influenced by the accumulation of nonsynonymous substitutions associated with the evolution of the NX-2 chemotype, and a significant (A < 0.001) change in selection pressure was observed along the NX-2 branch (co = 1.16) in comparison to other branches (omega = 0.17) in the TRI1 phylogeny. Parameter estimates were consistent with positive selection for specific amino acid changes during the evolution of NX-2, but direct tests of positive selection were not significant. Phylogenetic analyses of fourfold degenerate sites and intron sequences in TRI1 indicated the NX-2 chemotype had a single evolutionary origin and evolved recently from a type B ancestor. Our results indicate the NX-2 chemotype may be indigenous, and possibly endemic, to southern Canada and the northern U.S. In addition, we demonstrate that the evolution of TRH within FSAMSC-1 has been complex, with evidence of trans-species evolution and chemotype-specific shifts in selective constraint. Published by Elsevier Inc. C1 [Kelly, Amy; Proctor, Robert H.; Ward, Todd J.] ARS, USDA, 1815 North Univ St, Peoria, IL 61604 USA. [Belzile, Francois] Univ Laval, 1030 Ave Med, Quebec City, PQ G1V 0A6, Canada. [Chulze, Sofia N.] Univ Nacl Rio Cuarto, CONICET, Rutas 8 & 36 Km 601, RA-5800 Cordoba, Argentina. [Clear, Randall M.] 23 Healy Crescent, Winnipeg, MB R2N 2S6, Canada. [Cowger, Christina] North Carolina State Univ, ARS, USDA, 3411 Gardner Hall, Raleigh, NC 27695 USA. [Elmer, Wade] Connecticut Agr Expt Stn, 123 Huntington St, New Haven, CT 06504 USA. [Lee, Theresa] Natl Inst Agr Sci, 166 Nongsaengmyeong Ro, Wanju Gun 55365, Jeollabuk Do, South Korea. [Obanor, Friday] Commonwealth Sci & Ind Res Org, 306 Carmody Rd, St Lucia, Qld 4067, Australia. [Waalwijk, Cees] Plant Res Int, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. RP Ward, TJ (reprint author), ARS, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM todd.ward@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service National Program for Food Safety FX We thank N. Orwig and T. Usgaard for excellent technical support. We also are indebted to Kerry O'Donnell, Sukumar Chakraborty, Emerson Del Ponte, H. Corby Kistler, David Geiser, the Pennsylvania State University Fusarium Research Center, and the ARS Culture Collection (NRRL) for assistance in developing the isolate collections used in this study. This study was supported by the United States Department of Agriculture-Agricultural Research Service National Program for Food Safety. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 66 TC 0 Z9 0 U1 10 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD OCT PY 2016 VL 95 BP 39 EP 48 DI 10.1016/j.fgb.2016.08.003 PG 10 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DY1RJ UT WOS:000384871800005 PM 27497828 ER PT J AU Chan, DW Green, S Fiatarone Singh, M Barnard, R Cheema, BS AF Chan, Danwin Green, Simon Fiatarone Singh, Maria Barnard, Robert Cheema, Birinder S. TI Development, feasibility, and efficacy of a customized exercise device to deliver intradialytic resistance training in patients with end stage renal disease: Non-randomized controlled crossover trial SO HEMODIALYSIS INTERNATIONAL LA English DT Article DE Exercise; intradialytic; resistance training ID QUALITY-OF-LIFE; CHRONIC KIDNEY-DISEASE; RANDOMIZED CONTROLLED-TRIAL; HEMODIALYSIS-PATIENTS; PHYSICAL FUNCTION; MAINTENANCE HEMODIALYSIS; PHYSIOLOGICAL FACTORS; PROGRESSIVE EXERCISE; BODY-COMPOSITION; STRENGTH AB Introduction This study assessed the feasibility and efficacy of a novel resistance training device used within an intradialytic progressive resistance training (PRT) intervention. Methods Non-randomized, within-subjects crossover design with outcomes assessed at baseline (week 0), postcontrol (week 13) and post-PRT intervention (week 26). Twenty-two hemodialysis patients (59% men, 71 +/- 11 years) performed PRT three sessions per week for 12 weeks. The resistance training device was developed to enable the performance of 2 upper body and 3 lower body exercises, unilaterally and bilaterally, both before and during dialysis, with loads of 2.5 to 59 kg. Feasibility outcomes included adverse events, adherence and training load progression. Changes in upper and lower body muscular strength, six-minute walk, aspects of health-related quality of life (HRQoL) and depression were evaluated. Findings The PRT intervention was delivered without serious adverse events, resulted in 71.2% +/- 23.3% adherence and significant adaptation of all training loads from pre to mid to post training (83.8%-185.6%, all P < 0.05). Lower body strength (P < 0.001) and HRQoL subscales (Role-Physical, Social Functioning, Role-Emotional) significantly increased (all P < 0.01) and a trend toward reduced depression was noted (P = 0.06). No significant changes were noted in other outcomes. Discussion PRT using the novel resistance training device was feasible and improved measures of physical and psychological health. This device can be utilized in most dialysis centers. Future studies are required to evaluate dose-response effects of PRT prescriptions in subpopulations, and the translation of PRT to standard dialysis practice. C1 [Chan, Danwin; Green, Simon; Cheema, Birinder S.] Univ Western Sydney, Sch Sci & Hlth, Locked Bag 1797, Penrith, NSW 2751, Australia. [Fiatarone Singh, Maria] Univ Sydney, Fac Hlth Sci, Exercise Hlth & Performance Fac Res Grp, Sydney, NSW, Australia. [Fiatarone Singh, Maria] Univ Sydney, Sydney Med Sch, Sydney, NSW, Australia. [Fiatarone Singh, Maria] Tufts Univ, Hebrew SeniorLife & Jean Mayer USDA Human Nutr Ct, Boston, MA 02111 USA. [Barnard, Robert] Ctr Phys Act Ageing, Northfield, SA, Australia. [Cheema, Birinder S.] Natl Inst Complementary Med, Penrith, NSW, Australia. RP Chan, DW (reprint author), Univ Western Sydney, Sch Sci & Hlth, Locked Bag 1797, Penrith, NSW 2751, Australia. EM danwinchan@gmail.com OI Chan, Danwin/0000-0002-1833-4661 FU Western Sydney University; Australian Postgraduate Award FX This research was supported by postgraduate student funding provided by Western Sydney University. Danwin Chan was recipient of an Australian Postgraduate Award. NR 51 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1492-7535 EI 1542-4758 J9 HEMODIAL INT JI Hemodial. Int. PD OCT PY 2016 VL 20 IS 4 BP 650 EP 660 DI 10.1111/hdi.12432 PG 11 WC Urology & Nephrology SC Urology & Nephrology GA DY0UN UT WOS:000384811800020 PM 27283780 ER PT J AU Chen, YG Ulyshen, MD Poland, TM AF Chen, Yigen Ulyshen, Michael D. Poland, Therese M. TI Abundance of volatile organic compounds in white ash phloem and emerald ash borer larval frass does not attract Tetrastichus planipennisi in a Y-tube olfactometer SO INSECT SCIENCE LA English DT Article DE Agrilus planipennis; biological control; Fraxinus; invasive species; semiochemical ID GOSSYPIUM-HIRSUTUM L.; HOST LOCATION; AGRILUS-PLANIPENNIS; NITROGEN-FERTILIZATION; COLEOPTERA BUPRESTIDAE; FEEDING PREFERENCE; COTTON PLANT; PARASITOIDS; HYMENOPTERA; PHEROMONE AB Many natural enemies employ plant- and/or herbivore-derived signals for host/prey location. The larval parasitoid Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) is 1 of 3 biocontrol agents currently being released in an effort to control the emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coloeptera: Burprestidae) in NorthAmerica. To enhance its efficiency, allelochemicals that attract it need to be assessed. In this study, ash phloem volatile organic compounds (VOCs) of black, green, and white ash, and EAB larval frass were compared. Foraging behavior of T. planipennisi females in response to VOCs of white ash or frass from EAB larvae feeding on white ash phloem was tested using a Y-tube olfactometer. Results indicated that the 3 ash species had similar VOC profiles. EAB larval frass generally contained greater levels of VOCs than phloem. Factor analysis indicated that the 11 VOCs could be broadly divided into 2 groups, with alpha-bisabolol, beta-caryophyllene, (E)-2-hexenal, (Z)-3-hexenal, limonene, methyl benzoate, methyl indole-3-acetic acid, methyl jasmonate, methyl salicylate as the first group and the rest (i.e., methyl linoleate and methyl linolenate) as a second. Abundance of VOCs in white ash phloem tissue and frass, nevertheless, did not attract T. planipennisi females. The concealed feeding of EAB larvae might explain the selection for detectable and reliable virbrational signals, instead of undetectable and relatively unreliable VOC cues from phloem and frass, in short-range foraging by T. planipennisi. Alternatively, it is possible that T. planipennisi is not amenable to the Y-tube olfactometer assay employed. C1 [Chen, Yigen; Ulyshen, Michael D.] Michigan State Univ, Dept Entomol, 288 Farm Lane,Nat Sci Bldg,Room 243, E Lansing, MI 48824 USA. [Chen, Yigen] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. [Ulyshen, Michael D.] US Forest Serv, USDA, 320 Green St, Athens, GA 30602 USA. [Poland, Therese M.] US Forest Serv, USDA, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. RP Chen, YG (reprint author), Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. EM ygchen2007@gmail.com FU USDA Forest Service, NA FHP FX We thank Tina Ciaramitaro and Deborah Miller for maintenance of the EAB colony and trees in the greenhouse. We are indebted to Allard Cosse and Peter Silk for their insightful comments that have improved an early draft of the manuscript. We also thank two anonymous reviewers for their comments on the manuscript. The research was supported by the USDA Forest Service, NA FHP. NR 29 TC 1 Z9 1 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD OCT PY 2016 VL 23 IS 5 BP 712 EP 719 DI 10.1111/1744-7917.12227 PG 8 WC Entomology SC Entomology GA DX2DV UT WOS:000384178400006 PM 25879864 ER PT J AU Wren, DG Langendoen, EJ Kuhnle, RA AF Wren, Daniel G. Langendoen, Eddy J. Kuhnle, Roger A. TI Bed Topography and Sand Transport Responses to a Step Change in Discharge and Water Depth SO JOURNAL OF HYDRAULIC ENGINEERING LA English DT Article ID UNSTEADY FLOWS; SEDIMENT; RIVER; MORPHOLOGY; ROUGHNESS; DUNES; SEMIVARIOGRAM; FORMULATION; TURBULENCE; EVOLUTION AB At base-flow conditions, ephemeral streams with mobile beds may inherit bed topography caused by previous higher flow events, resulting in bed topography that is not in equilibrium with flow conditions. Major flow events can generate large bedforms that result in hydrodynamics and sediment transport that are not in equilibrium with subsequent lower flow conditions. To simulate a large storm runoff-driven stream flow followed by lower base-flow conditions, changes in sand bed topography and sand transport caused by a simultaneous reduction in discharge and flow depth in a laboratory flume with an equilibrium sand bed and steady, approximately uniform, flow were investigated. Bed topography was evaluated using second-order structure functions, semivariograms, and statistical analyses. Sediment transport responded rapidly to a near-instantaneous negative step in mean flow depth and discharge. The relative contributions of reduced bedform height and celerity to the decreased transport rate are quantified, and bedform celerity was the primary contributor to reductions in transport rate. To evaluate the effect of initial bed topography on equilibrium topography, a flat bed initial condition was subjected to the same flow as was applied after the negative step. The equilibrium bed topography for the flat bed initial condition was similar to the topography that was formed after the negative step. Semivariogram analysis provided a reliable estimate of mean bedform height without the need for detecting individual bedforms. Total sediment load following a negative step in discharge and flow depth followed an exponential decay model that resulted from the combined exponential decay of bedform celerity and bedform height. C1 [Wren, Daniel G.; Langendoen, Eddy J.; Kuhnle, Roger A.] USDA ARS, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. RP Wren, DG (reprint author), USDA ARS, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. EM Daniel.Wren@ars.usda.gov NR 34 TC 0 Z9 0 U1 7 U2 7 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 EI 1943-7900 J9 J HYDRAUL ENG JI J. Hydraul. Eng.-ASCE PD OCT PY 2016 VL 142 IS 10 AR 04016040 DI 10.1061/(ASCE)HY.1943-7900.0001172 PG 12 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA DX3CI UT WOS:000384250100011 ER PT J AU Burke, MP Martini, LH Cayir, E Hartline-Grafton, HL Meade, RL AF Burke, Michael P. Martini, Lauren H. Cayir, Ebru Hartline-Grafton, Heather L. Meade, Randa L. TI Severity of Household Food Insecurity Is Positively Associated with Mental Disorders among Children and Adolescents in the United States SO JOURNAL OF NUTRITION LA English DT Article DE food insecurity; mental disorder; children; adolescents; National Health Interview Survey; NHIS ID DIFFICULTIES QUESTIONNAIRE; U.S. ADOLESCENTS; NATIONAL SAMPLE; HEALTH; DEPRESSION; BEHAVIOR; VIOLENCE; SUICIDE; FAMILY; INSUFFICIENCY AB Background: Household food insecurity and mental disorders are both prevalent conditions among children and adolescents (i.e., youth) in the United States. Although some research has examined the association between the 2 conditions, it is not known whether more severe food insecurity is differently associated with mental disorders in youth. Objective: We investigated the association between severity of household food insecurity and mental disorders among children (aged 4-11 y) and adolescents (aged 12-17 y) using valid and reliable measures of both household food security status and mental disorders. Methods: We analyzed cross-sectional data on 16,918 children and 14,143 adolescents whose families participated in the 20112014 National Health Interview Survey. The brief Strengths and Difficulties Questionnaire and the 10-item USDA Household Food Security Survey Module were used to measure mental disorders and food security status, respectively. Multinomial logistic regressions were used to test the association between household food security status and mental disorders in youth. Results: There was a significant linear trend in ORs, such that as severity of household food insecurity increased so did the odds of youth having a mental disorder (P < 0.001). Other selected results included the following: compared with food secure households, youth in marginally food-secure households had higher odds of having a mental disorder with impairment [child OR: 1.26 (95% CI: 1.05, 1.52); adolescent OR: 1.33 (95% CI: 1.05, 1.68)]. In addition, compared with food-secure households, youth in very-low-food-secure households had higher odds of having a mental disorder with severe impairment [child OR: 2.55 (95% CI: 1.90, 3.43); adolescent OR: 3.44 (95% CI: 2.50, 4.75)]. Conclusions: The severity of household food insecurity is positively associated with mental disorders among both children and adolescents in the United States. These results suggest that improving household food security status has the potential to reduce mental disorders among US youth. C1 [Burke, Michael P.] USDA, Food & Nutr Serv, Alexandria, VA 22302 USA. [Burke, Michael P.; Martini, Lauren H.] Univ South Carolina, Arnold Sch Publ Hlth, Ctr Res Nutr & Hlth Dispar, Columbia, SC 29208 USA. [Martini, Lauren H.] Univ South Carolina, Arnold Sch Publ Hlth, Dept Epidemiol & Biostat, Columbia, SC USA. [Cayir, Ebru] Univ South Carolina, Arnold Sch Publ Hlth, Dept Hlth Promot Educ & Behav, Columbia, SC USA. [Hartline-Grafton, Heather L.] Food Res & Act Ctr, Washington, DC USA. [Meade, Randa L.] Univ Tennessee, Dept Nutr, Knoxville, TN 37996 USA. RP Burke, MP (reprint author), USDA, Food & Nutr Serv, Alexandria, VA 22302 USA.; Burke, MP (reprint author), Univ South Carolina, Arnold Sch Publ Hlth, Ctr Res Nutr & Hlth Dispar, Columbia, SC 29208 USA. EM michael.burke@fnsusda.gov NR 42 TC 0 Z9 0 U1 7 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD OCT PY 2016 VL 146 IS 10 BP 2019 EP 2026 DI 10.3945/jn.116.232298 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DY0KE UT WOS:000384784900012 PM 27581581 ER PT J AU Abdelrahman, HM Olk, DC Dinnes, D Ventrella, D Miano, T Cocozza, C AF Abdelrahman, Hamada M. Olk, Dan C. Dinnes, Dana Ventrella, Domenico Miano, Teodoro Cocozza, Claudio TI Occurrence and abundance of carbohydrates and amino compounds in sequentially extracted labile soil organic matter fractions SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Light fraction; Mobile humic acid; Organic farming; Particulate organic matter; SOM sequential extraction ID CHROMATOGRAPHY-PULSED AMPEROMETRY; SOLID-STATE NMR; HUMIC ACIDS; CHEMICAL-COMPOSITION; MICROBIAL BIOMASS; PLANT MATERIALS; COMPONENTS; CARBON; MANAGEMENT; QUALITY AB The study aimed to describe the carbohydrates and amino compounds content in soil, the light fraction (LF), the > 53 mu m particulate organic matter (POM), and the mobile humic acid (MHA) fraction and to find out whether the carbohydrates and amino compounds can be used to explain the origin of SOM fractions. Soil samples were collected from two agricultural fields managed under organic farming in southern Italy. The LF, the POM, and the MHA were sequentially extracted from each soil sample then characterized. Seven neutral sugars and 19 amino compounds (amino acids and amino sugars) were determined in each soil sample and its correspondent fractions. The MHA contained less carbohydrate than the LF or the POM but its carbohydrates, although dominated by arabinose, were relatively with larger microbial contribution as revealed by the mannose/xylose ratio. The amino compounds were generally less in the LF or the POM than in the MHA, while the fungal (aspartic and serine) and bacterial (alanine and glycine) amino acids were larger in the MHA than in the LF or the POM, underlining the microbial contribution to the MHA. Results from both sites indicated that total carbohydrates content decreased moving from the LF (younger fraction) to the MHA (older fraction), which seems to follow a decomposition continuum of organic matter in the soil-plant system. The study showed that the MHA is a labile humified fraction of soil C due to its content of carbohydrates and concluded that the content of carbohydrates and amino compounds in the LF, the POM and the MHA can depict the nature of these fractions and their cycling pattern and response to land management. C1 [Abdelrahman, Hamada M.; Olk, Dan C.; Dinnes, Dana] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Abdelrahman, Hamada M.] Cairo Univ, Dept Soil Sci, Fac Agr, Giza 12613, Egypt. [Ventrella, Domenico] CREA SCA, Unita Ric Sistemi Colturali Ambienti Caldo Aridi, Bari, Italy. [Miano, Teodoro; Cocozza, Claudio] Univ Bari Aldo Moro, DiSSPA, I-70126 Bari, Italy. RP Abdelrahman, HM (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA.; Abdelrahman, HM (reprint author), Cairo Univ, Dept Soil Sci, Fac Agr, Giza 12613, Egypt. EM hamada@uniba.it RI Abdelrahman, Hamada /D-2137-2009 OI Abdelrahman, Hamada /0000-0002-6069-7239 FU Ministero delle Politiche Agricole Alimentari e Forestali, Italy FX This work was carried out in the framework of the BIO.INNOVA project financed by the Ministero delle Politiche Agricole Alimentari e Forestali, Italy. The authors acknowledge the Apulia Region for using scientific instrumentation acquired with RELA-VALBIOR project. The authors are grateful to anonymous reviewers for their valuable comments and suggestions on the manuscript draft. NR 40 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD OCT PY 2016 VL 16 IS 10 BP 2375 EP 2384 DI 10.1007/s11368-016-1437-y PG 10 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA DX7NZ UT WOS:000384576200005 ER PT J AU Zerofsky, M Ryder, M Bhatia, S Stephensen, CB King, J Fung, EB AF Zerofsky, Melissa Ryder, Mark Bhatia, Suruchi Stephensen, Charles B. King, Janet Fung, Ellen B. TI Effects of early vitamin D deficiency rickets on bone and dental health, growth and immunity SO MATERNAL AND CHILD NUTRITION LA English DT Article DE rickets; vitamin D; bone mineral density; dental health; gingival infection; bone fracture ID NUTRITIONAL RICKETS; D SUPPLEMENTATION; EARLY-CHILDHOOD; BIRTH-COHORT; PREGNANCY; RISK; CHILDREN; INFANTS; ASTHMA; INFECTION AB Vitamin D deficiency is associated with adverse health outcomes, including impaired bone growth, gingival inflammation and increased risk for autoimmune disease, but the relationship between vitamin D deficiency rickets in childhood and long-term health has not been studied. In this study, we assessed the effect of early vitamin D deficiency on growth, bone density, dental health and immune function in later childhood to determine if children previously diagnosed with rickets were at greater risk of adverse health outcomes compared with healthy children. We measured serum 25-hydroxyvitamin D, calcium, parathyroid hormone, bone mineral density, anthropometric measures, dietary habits, dental health, general health history, and markers of inflammation in 14 previously diagnosed rickets case children at Children's Hospital Oakland Research Center. We compared the findings in the rickets cases with 11 healthy children selected from the population of CHO staff families. Fourteen mothers of the rickets cases, five siblings of the rickets cases, and seven mothers of healthy children also participated. Children diagnosed with vitamin D deficiency rickets had a greater risk of fracture, greater prevalence of asthma, and more dental enamel defects compared with healthy children. Given the widespread actions of vitamin D, it is likely that early-life vitamin D deficiency may increase the risk of disease later in childhood. Further assessment of the long-term health effects of early deficiency is necessary to make appropriate dietary recommendations for infants at risk of deficiency. C1 [Zerofsky, Melissa] UC Davis Grad Grp Nutr Biol, Davis, CA USA. [Zerofsky, Melissa; Stephensen, Charles B.] USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. [Ryder, Mark] UC San Francisco Sch Dent, Div Periodontol, San Francisco, CA USA. [Bhatia, Suruchi; King, Janet; Fung, Ellen B.] Childrens Hosp Oakland, Res Inst, Oakland, CA 94609 USA. RP Zerofsky, M (reprint author), USDA, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM mszerofsky@ucdavis.edu FU National Center for Advancing Translational Sciences, National Institutes of Health, through UCSF-CTSI [UL1 TR000004] FX This project was supported by the National Center for Advancing Translational Sciences, National Institutes of Health, through UCSF-CTSI Grant Number UL1 TR000004. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 36 TC 2 Z9 2 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1740-8695 EI 1740-8709 J9 MATERN CHILD NUTR JI Matern. Child Nutr. PD OCT PY 2016 VL 12 IS 4 BP 898 EP 907 DI 10.1111/mcn.12187 PG 10 WC Nutrition & Dietetics; Pediatrics SC Nutrition & Dietetics; Pediatrics GA DX8QA UT WOS:000384652400021 PM 25850574 ER PT J AU Haas, M Menke, J Chao, S Steffenson, BJ AF Haas, Matthew Menke, Jon Chao, Shiaoman Steffenson, Brian J. TI Mapping quantitative trait loci conferring resistance to a widely virulent isolate of Cochliobolus sativus in wild barley accession PI 466423 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MULTIPLE DISEASE RESISTANCE; SPOT BLOTCH RESISTANCE; NORTH-DAKOTA; QTL-ANALYSIS; DIVERSITY; REGISTRATION; POPULATION; SPONTANEUM; PATHOTYPES; RESPONSES AB This research characterized the genetics of resistance of wild barley accession PI 466423 to a widely virulent pathotype of Cochliobolus sativus . Breeding lines were identified that combine the Midwest Six-rowed Durable Resistance Haplotype and resistance to the virulent isolate ND4008. Spot blotch, caused by Cochliobolus sativus, is a historically important foliar disease of barley (Hordeum vulgare L.) in the Upper Midwest region of the USA. However, for the last 50 years this disease has been of little consequence due to the deployment of resistant six-rowed malting cultivars. These durably resistant cultivars carry the Midwest Six-rowed Durable Resistant Haplotype (MSDRH) comprised of three Quantitative Trait Loci (QTL) on chromosomes 1H, 3H and 7H, originally contributed by breeding line NDB112. Recent reports of C. sativus isolates (e.g. ND4008) with virulence on NDB112 indicate that widely grown cultivars of the region are vulnerable to spot blotch epidemics. Wild barley (H. vulgare ssp. spontaneum), the progenitor of cultivated barley, is a rich source of novel alleles, especially for disease resistance. Wild barley accession PI 466423 is highly resistant to C. sativus isolate ND4008. To determine the genetic architecture of resistance to isolate ND4008 in PI 466423, we phenotyped and genotyped an advanced backcross population (N = 244) derived from the wild accession and the recurrent parent 'Rasmusson', a Minnesota cultivar with the MSDRH. Disease phenotyping was done on BC2F4 seedlings in the greenhouse using isolate ND4008. The Rasmusson/PI 466423 population was genotyped with 7842 single nucleotide polymorphic markers. QTL analysis using composite interval mapping revealed four resistance loci on chromosomes 1H, 2H, 4H and 5H explaining 10.3, 7.4, 6.4 and 8.4 % of the variance, respectively. Resistance alleles on chromosomes 1H, 4H and 5H were contributed by PI 466423, whereas the one on chromosome 2H was contributed by Rasmusson. All four resistance QTL are likely coincident with previously identified QTL. Agronomically advanced two- and six-rowed lines combining the MSDRH and resistance alleles to isolate ND4008 have been identified and are being utilized in breeding. These results reaffirm the value of using wild relatives as a source of novel resistance alleles. C1 [Haas, Matthew; Menke, Jon; Steffenson, Brian J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Chao, Shiaoman] USDA ARS, Cereal Crop Res Unit, Fargo, ND 58102 USA. RP Steffenson, BJ (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM bsteffen@umn.edu FU Lieberman-Okinow Endowment at the University of Minnesota; USDA-NIFA [2011-68002-30029]; Minnesota Discovery, Research and Innovation Economy (MnDRIVE) graduate student fellowship through the University of Minnesota Informatics Institute FX This work was supported by funding from the Lieberman-Okinow Endowment at the University of Minnesota, the USDA-NIFA sponsored Triticeae-CAP project (2011-68002-30029) and the Minnesota Discovery, Research and Innovation Economy (MnDRIVE) graduate student fellowship to M.H. through the University of Minnesota Informatics Institute. We thank the Minnesota Supercomputing Institute for hosting software utilized in analysis of data included in this publication. The authors would also like to thank Ben Alsop, Stephanie Dahl, Pablo Olivera, and Tamas Szinyei for population development. NR 32 TC 0 Z9 0 U1 8 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD OCT PY 2016 VL 129 IS 10 BP 1831 EP 1842 DI 10.1007/s00122-016-2742-y PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DW5UG UT WOS:000383711900002 PM 27316436 ER PT J AU Del Valle-Echevarria, AR Sanseverino, W Garcia-Mas, J Havey, MJ AF Del Valle-Echevarria, A. R. Sanseverino, W. Garcia-Mas, J. Havey, M. J. TI Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GENOME; CHLOROPLAST; REARRANGEMENTS; RECOMBINATION; TRANSMISSION; CONTRIBUTE; SEQUENCES; REVEALS; DNA AB Pentatricopeptide repeat (PPR) 336 was identified as the candidate gene for Paternal Sorting of Mitochondria ( Psm ), a nuclear locus that affects the predominant mitochondria transmitted to progenies. Cucumber (Cucumis sativus L.) is a useful plant to study organellar-nuclear interactions because its organelles show differential transmission, maternal for chloroplasts and paternal for mitochondria. The mitochondrial DNA (mtDNA) of cucumber is relatively large due in part to accumulation of repetitive DNAs and recombination among these repetitive regions produces structurally polymorphic mtDNAs associated with paternally transmitted mosaic (MSC) phenotypes. The mitochondrial mutant MSC16 possesses an under-representation of ribosomal protein S7 (rps7), a key component of the small ribosomal subunit in the mitochondrion. A nuclear locus, Paternal Sorting of Mitochondria (Psm), affects the predominant mitochondria transmitted to progenies generated from crosses with MSC16 as the male parent. Using single nucleotide polymorphisms, Psm was mapped to a 170 kb region on chromosome 3 of cucumber and pentatricopeptide repeat (PPR) 336 was identified as the likely candidate gene. PPR336 stabilizes mitochondrial ribosomes in Arabidopsis thaliana and because MSC16 shows reduced transcription of rps7, the cucumber homolog of PPR336 (CsPPR336) as the candidate for Psm is consistent with a nuclear effect on ribosome assembly or stability in the mitochondrion. We used polymorphisms in CsPPR336 to genotype progenies segregating at Psm and recovered only one Psm -/- plant with the MSC phenotype, indicating that the combination of the Psm- allele with mitochondria from MSC16 is almost always lethal. This research illustrates the usefulness of the MSC mutants of cucumber to reveal and study unique interactions between the mitochondrion and nucleus. C1 [Del Valle-Echevarria, A. R.; Havey, M. J.] Univ Wisconsin Madison, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Sanseverino, W.; Garcia-Mas, J.] CSIC IRTA UAB UB, CRAG, IRTA, Barcelona 08193, Spain. [Havey, M. J.] Univ Wisconsin Madison, USDA ARS, 1575 Linden Dr, Madison, WI 53706 USA. RP Havey, MJ (reprint author), Univ Wisconsin Madison, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Havey, MJ (reprint author), Univ Wisconsin Madison, USDA ARS, 1575 Linden Dr, Madison, WI 53706 USA. EM mjhavey@wisc.edu OI Sanseverino, Walter/0000-0003-3324-5912 FU Agricultural Research Service of the United States Department of Agriculture; Science and Medicine Graduate Research Scholars Fellowship; Advance Opportunity Fellowship of the Graduate School of the University of Wisconsin-Madison; National Institutes of Health (NIH) Biotechnology Predoctoral Training Program (BTP) of the University of Wisconsin-Madison (NIH) [5 T32 GM08349] FX This research was supported by the Agricultural Research Service of the United States Department of Agriculture, a Science and Medicine Graduate Research Scholars Fellowship, an Advance Opportunity Fellowship of the Graduate School of the University of Wisconsin-Madison, and the National Institutes of Health (NIH) Biotechnology Predoctoral Training Program (BTP) of the University of Wisconsin-Madison (NIH Grant 5 T32 GM08349). NR 38 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD OCT PY 2016 VL 129 IS 10 BP 1951 EP 1959 DI 10.1007/s00122-016-2751-x PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DW5UG UT WOS:000383711900010 PM 27423873 ER PT J AU Gao, ML Hu, LL Li, YH Weng, YQ AF Gao, Meiling Hu, Liangliang Li, Yuhong Weng, Yiqun TI The chlorophyll-deficient golden leaf mutation in cucumber is due to a single nucleotide substitution in CsChlI for magnesium chelatase I subunit SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MG-CHELATASE; TETRAPYRROLE BIOSYNTHESIS; ARABIDOPSIS-THALIANA; CHLOROPLAST DEVELOPMENT; LIGHT INDUCTION; CHLD SUBUNITS; GENE; BARLEY; MUTANT; RICE AB The cucumber chlorophyll-deficient golden leaf mutation is due to a single nucleotide substitution in the CsChlI gene for magnesium chelatase I subunit which plays important roles in the chlorophyll biosynthesis pathway. The Mg-chelatase catalyzes the insertion of Mg2+ into the protoporphyrin IX in the chlorophyll biosynthesis pathway, which is a protein complex encompassing three subunits CHLI, CHLD, and CHLH. Chlorophyll-deficient mutations in genes encoding the three subunits have played important roles in understanding the structure, function and regulation of this important enzyme. In an EMS mutagenesis population, we identified a chlorophyll-deficient mutant C528 with golden leaf color throughout its development which was viable and able to set fruits and seeds. Segregation analysis in multiple populations indicated that this leaf color mutation was recessively inherited and the green color showed complete dominance over golden color. Map-based cloning identified CsChlI as the candidate gene for this mutation which encoded the CHLI subunit of cucumber Mg-chelatase. The 1757-bp CsChlI gene had three exons and a single nucleotide change (G to A) in its third exon resulted in an amino acid substitution (G269R) and the golden leaf color in C528. This mutation occurred in the highly conserved nucleotide-binding domain of the CHLI protein in which chlorophyll-deficient mutations have been frequently identified. The mutant phenotype, CsChlI expression pattern and the mutated residue in the CHLI protein suggested the mutant allele in C528 is unique among mutations identified so far in different species. This golden leaf mutant not only has its potential in cucumber breeding, but also provides a useful tool in understanding the CHLI function and its regulation in the chlorophyll biosynthesis pathway as well as chloroplast development. C1 [Gao, Meiling; Weng, Yiqun] Qiqihar Univ, Coll Life Sci Agr & Forestry, Qiqihar 161006, Peoples R China. [Gao, Meiling] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Hu, Liangliang; Li, Yuhong] Northwest A&F Univ, Hort Coll, Yangling 712100, Peoples R China. [Weng, Yiqun] USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. RP Gao, ML; Weng, YQ (reprint author), Qiqihar Univ, Coll Life Sci Agr & Forestry, Qiqihar 161006, Peoples R China.; Gao, ML (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Weng, YQ (reprint author), USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM gaomeiling0539@163.com; yiqun.weng@ars.usda.gov FU National Natural Science Foundation of China [31401891, 31171955, 31471891]; Natural Science Foundation of Heilongjiang Province [C201330]; Young Outstanding Faculty Support Program in General Higher Education Institutions of Heilongjiang Province [1155G65]; US Department of Agriculture Specialty Crop Research Initiative Grant [2011-51181-30661] FX The authors thank Kristin Haider for technical help. MG's work was supported by the National Natural Science Foundation of China (No. 31401891), the Natural Science Foundation of Heilongjiang Province (C201330) and Young Outstanding Faculty Support Program in General Higher Education Institutions of Heilongjiang Province (1155G65). The work in YL's lab was supported by the National Natural Science Foundation of China (31171955 and 31471891). The research in YW's lab was supported by US Department of Agriculture Specialty Crop Research Initiative Grant (Project Number 2011-51181-30661). NR 61 TC 0 Z9 0 U1 28 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD OCT PY 2016 VL 129 IS 10 BP 1961 EP 1973 DI 10.1007/s00122-016-2752-9 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DW5UG UT WOS:000383711900011 PM 27435733 ER PT J AU Lou, FF DiCaprio, E Li, XH Dai, XJ Ma, YM Hughes, J Chen, HQ Kingsley, DH Li, JR AF Lou, Fangfei DiCaprio, Erin Li, Xinhui Dai, Xianjun Ma, Yuanmei Hughes, John Chen, Haiqiang Kingsley, David H. Li, Jianrong TI Variable High-Pressure-Processing Sensitivities for Genogroup II Human Noroviruses SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HIGH HYDROSTATIC-PRESSURE; HEPATITIS-A VIRUS; PORCINE GASTRIC MUCIN; FELINE CALICIVIRUS; UNITED-STATES; INACTIVATION; TEMPERATURE; GASTROENTERITIS; SURROGATE; PH AB Human norovirus (HuNoV) is a leading cause of foodborne diseases worldwide. High-pressure processing (HPP) is one of the most promising nonthermal technologies for the decontamination of viral pathogens in foods. However, the survival of HuNoVs after HPP is poorly understood because these viruses cannot be propagated in vitro. In this study, we estimated the survival of different HuNoV strains within genogroup II (GII) after HPP treatment using viral receptor-binding ability as an indicator. Four HuNoV strains (one GII genotype 1 [GII. 1] strain, two GII. 4 strains, and one GII. 6 strain) were treated at high pressures ranging from 200 to 600 MPa. After treatment, the intact viral particles were captured by porcine gastric mucin-conjugated magnetic beads (PGM-MBs) that contained histo-blood group antigens, the functional receptors for HuNoVs. The genomic RNA copies of the captured HuNoVs were quantified by real-time reverse transcriptase PCR (RT-PCR). Two GII. 4 HuNoVs had similar sensitivities to HPP. The resistance of HuNoV strains against HPP ranked as follows: GII. 1 > GII. 6 > GII. 4, with GII. 4 being the most sensitive. Evaluation of temperature and matrix effects on HPP-mediated inactivation of HuNoV GII. 4, GII. 1, and GII. 6 strains showed that HuNoV was more easily inactivated at lower temperatures and at a neutral pH. In addition, phosphate-buffered saline (PBS) and minimal essential medium (MEM) can provide protective effects against HuNoV inactivation compared to H2O. Collectively, this study demonstrated that (i) different HuNoV strains within GII exhibited different sensitivities to high pressure, and (ii) HPP is capable of inactivating HuNoV GII strains by optimizing pressure parameters. C1 [Lou, Fangfei; DiCaprio, Erin; Ma, Yuanmei; Li, Jianrong] Ohio State Univ, Coll Vet Med, Dept Vet Biosci, Columbus, OH 43210 USA. [Lou, Fangfei] Ohio State Univ, Program Food Sci & Technol, Columbus, OH 43210 USA. [Hughes, John] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA. [Li, Xinhui; Chen, Haiqiang] Univ Delaware, Dept Anim & Food Sci, Newark, DE USA. [Dai, Xianjun] China Jiliang Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China. [Kingsley, David H.] Delaware State Univ, USDA ARS, Dover, DE USA. [DiCaprio, Erin] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Li, Xinhui] Univ Wisconsin, Dept Microbiol, La Crosse, WI 54601 USA. RP Li, JR (reprint author), Ohio State Univ, Coll Vet Med, Dept Vet Biosci, Columbus, OH 43210 USA. EM li.926@osu.edu RI Lou, Fangfei/J-9563-2014; OI Lou, Fangfei/0000-0002-9335-8209; Li, Xinhui/0000-0003-1568-1999 FU U.S. Department of Agriculture (USDA) [2015-69003-23410, 2011-68003-30005, 2011-68003-30395] FX This work, including the efforts of Jianrong Li, was funded by U.S. Department of Agriculture (USDA) (2015-69003-23410, 2011-68003-30005, and 2011-68003-30395). NR 36 TC 0 Z9 0 U1 9 U2 9 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD OCT PY 2016 VL 82 IS 19 BP 6037 EP 6045 DI 10.1128/AEM.01575-16 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DX0JX UT WOS:000384048700031 PM 27474724 ER PT J AU Li, XJ Lin, WC Chang, S Zhao, P Zhang, XH Liu, Y Chen, WG Li, BH Shu, DM Zhang, HM Chen, F Xie, QM AF Li, Xinjian Lin, Wencheng Chang, Shuang Zhao, Peng Zhang, Xinheng Liu, Yang Chen, Weiguo Li, Baohong Shu, Dingming Zhang, Huanmin Chen, Feng Xie, Qingmei TI Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China SO ARCHIVES OF VIROLOGY LA English DT Article ID MAREKS-DISEASE VACCINES; LONG TERMINAL REPEAT; COMPLETE GENOME SEQUENCE; GLIOMA-INDUCING VIRUS; ROUS-SARCOMA VIRUS; FOWL GLIOMA; CELL-LINE; CHICKENS; INFECTION; STRAIN AB Avian leukosis virus (ALV) causes high mortality associated with tumor formation and decreased fertility, and results in major economic losses in the poultry industry worldwide. Recently, a putative novel ALV subgroup virus named ALV-K was observed in Chinese local chickens. In this study, a novel ALV strain named GD14LZ was isolated from a Chinese local yellow broiler in 2014. The proviral genome was sequenced and phylogenetically analyzed. The replication ability and pathogenicity of this virus were also evaluated. The complete proviral genome sequence of GD14LZ was 7482 nt in length, with a genetic organization typical of replication-competent type C retroviruses lacking viral oncogenes. Sequence analysis showed that the gag, pol and gp37 genes of GD14LZ have high sequence similarity to those of other ALV strains (A-E subgroups), especially to those of ALV-E. The gp85 gene of the GD14LZ isolate showed a low sequence similarity to those other ALV strains (A-E subgroups) but showed high similarity to strains previously described as ALV-K. Phylogenetic analysis of gp85 also suggested that the GD14LZ isolate was related to ALV-K strains. Further study showed that this isolate replicated more slowly and was less pathogenic than other ALV strains. These results indicate that the GD14LZ isolate belongs to the novel subgroup ALV-K and probably arose by recombination of ALV-K with endogenous viruses with low replication and pathogenicity. This virus might have existed in local Chinese chickens for a long time. C1 [Li, Xinjian; Lin, Wencheng; Zhang, Xinheng; Liu, Yang; Chen, Weiguo; Chen, Feng; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, 483 Wushan Rd, Guangzhou, Guangdong, Peoples R China. [Li, Xinjian; Lin, Wencheng; Zhang, Xinheng; Liu, Yang; Chen, Weiguo; Chen, Feng; Xie, Qingmei] Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China. [Li, Xinjian; Lin, Wencheng; Zhang, Xinheng; Liu, Yang; Chen, Weiguo; Chen, Feng; Xie, Qingmei] Minist Agr, Key Lab Chicken Genet, Guangzhou 510642, Guangdong, Peoples R China. [Chang, Shuang; Zhao, Peng] Shandong Agr Univ, Coll Vet Med, Tai An 271018, Shandong, Peoples R China. [Li, Baohong; Shu, Dingming] Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Guangdong, Peoples R China. [Zhang, Huanmin] USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. [Lin, Wencheng; Chen, Feng; Xie, Qingmei] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China. [Lin, Wencheng; Xie, Qingmei] South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, 483 Wushan Rd, Guangzhou, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Minist Agr, Key Lab Chicken Genet, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou 510642, Guangdong, Peoples R China.; Xie, QM (reprint author), South China Collaborat Innovat Ctr Poultry Dis Co, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU Natural Science Foundation of Guangdong Province [S2013030013313]; National Natural Science Foundation of China [31472217]; Guangdong Province Science and Technology Plan Project [2012B020306002, 2012B091100078] FX This study was supported by the Natural Science Foundation of Guangdong Province (Grant No. S2013030013313), the National Natural Science Foundation of China (Grant No. 31472217), and the Guangdong Province Science and Technology Plan Project (Grant No. 2012B020306002, 2012B091100078). NR 35 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD OCT PY 2016 VL 161 IS 10 BP 2717 EP 2725 DI 10.1007/s00705-016-2965-x PG 9 WC Virology SC Virology GA DV1LE UT WOS:000382681800008 PM 27422398 ER PT J AU Adams, MJ Lefkowitz, EJ King, AMQ Harrach, B Harrison, RL Knowles, NJ Kropinski, AM Krupovic, M Kuhn, JH Mushegian, AR Nibert, M Sabanadzovic, S Sanfacon, H Siddell, SG Simmonds, P Varsani, A Zerbini, FM Gorbalenya, AE Davison, AJ AF Adams, Michael J. Lefkowitz, Elliot J. King, Andrew M. Q. Harrach, Balazs Harrison, Robert L. Knowles, Nick J. Kropinski, Andrew M. Krupovic, Mart Kuhn, Jens H. Mushegian, Arcady R. Nibert, Max Sabanadzovic, Sead Sanfacon, Helene Siddell, Stuart G. Simmonds, Peter Varsani, Arvind Zerbini, Francisco Murilo Gorbalenya, Alexander E. Davison, Andrew J. TI Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016) SO ARCHIVES OF VIROLOGY LA English DT Article AB This article lists the changes to virus taxonomy approved and ratified by the International Committee on Taxonomy of Viruses (ICTV) in April 2016. Changes to virus taxonomy (the Universal Scheme of Virus Classification of the International Committee on Taxonomy of Viruses [ICTV]) now take place annually and are the result of a multi-stage process. In accordance with the ICTV Statutes http://www.ictvonline.org/statutes.asp), proposals submitted to the ICTV Executive Committee (EC) undergo a review process that involves input from the ICTV Study Groups (SGs) and Subcommittees (SCs), other interested virologists, and the EC. After final approval by the EC, proposals are then presented for ratification to the full ICTV membership by publication on an ICTV web site (http://www.ictvonline.org/) followed by an electronic vote. The latest set of proposals approved by the EC was made available on the ICTV website by January 2016 (https://talk.ictvonline.org/files/proposals/). A list of these proposals was then emailed on 28 March 2016 to the 148 members of ICTV, namely the EC Members, Life Members, ICTV Subcommittee Members (including the SG chairs) and ICTV National Representatives. Members were then requested to vote on whether to ratify the taxonomic proposals (voting closed on 29 April 2016). C1 [Adams, Michael J.] 24 Woodland Way, Stevenage SG2 8BT, Herts, England. [Lefkowitz, Elliot J.] UAB, Dept Microbiol, BBRB 276,845 19th ST South, Birmingham, AL 35294 USA. [King, Andrew M. Q.] Sunfield, Dawney Hill, Woking GU24 0JB, Surrey, England. [Harrach, Balazs] Hungarian Acad Sci, Vet Med Res Inst, Hungaria Krt 21, H-1143 Budapest, Hungary. [Harrison, Robert L.] USDA, Invas Insect Biocontrol & Behav Lab, 10300 Baltimore Ave,Bldg 007 Barc West, Beltsville, MD 20705 USA. [Knowles, Nick J.] Pirbright Inst, Ash Rd, Pirbright GU24 0NF, Surrey, England. [Kropinski, Andrew M.] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Kropinski, Andrew M.] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. [Krupovic, Mart] Inst Pasteur, Dept Microbiol, 25 Rue Dr Roux, F-75015 Paris, France. [Kuhn, Jens H.] NIAID, NIH, DCR Integrated Res Facil, Ft Detrick IRF Frederick, B-8200 Res Plaza, Frederick, MD 21702 USA. [Mushegian, Arcady R.] Natl Sci Fdn, Div Mol & Cellular Biosci, 4201 Wilson Blvd, Arlington, VA 22230 USA. [Nibert, Max] Harvard Med Sch, Dept Microbiol & Immunobiol, 77 Ave Louis Pasteur, Boston, MA 02115 USA. [Sabanadzovic, Sead] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, 100 Old Hwy 12 Mail Stop 9775, Mississippi State, MS 39762 USA. [Sanfacon, Helene] Agr & Agri Food Canada, Summerland Res & Dev Ctr, 4200 Highway 97, Summerland, BC V0H 1Z0, Canada. [Siddell, Stuart G.] Univ Bristol, Dept Cellular & Mol Med, Fac Biomed Sci, Univ Walk, Bristol BS8 1TD, Avon, England. [Simmonds, Peter] Univ Oxford, Nuffield Dept Expt Med, Peter Medawar Bldg,South Pk Rd, Oxford OX1 3PS, England. [Varsani, Arvind] Univ Canterbury, Sch Biol Sci, Private Bag 4800, Christchurch 8140, New Zealand. [Varsani, Arvind] Univ Canterbury, Biomol Interact Ctr, Private Bag 4800, Christchurch 8140, New Zealand. [Zerbini, Francisco Murilo] Univ Fed Vicosa, Dept Fitopatol, BIOAGRO, BR-36570900 Vicosa, MG, Brazil. [Gorbalenya, Alexander E.] Leiden Univ, Dept Med Microbiol, Med Ctr, POB 9600,E4-P,Rm E4-72, NL-2300 RC Leiden, Netherlands. [Davison, Andrew J.] Univ Glasgow, Ctr Virus Res, MRC, Sir Michael Stoker Bldg,464 Bearsden Rd, Glasgow G61 1QH, Lanark, Scotland. RP Adams, MJ (reprint author), 24 Woodland Way, Stevenage SG2 8BT, Herts, England. EM mike.adams.ictv@gmail.com; elliotl@uab.edu; amqking@gmail.com; harrach@vmri.hu; robert.l.harrison@ars.usda.gov; nick.knowles@pirbright.ac.uk; Phage.Canada@gmail.com; mart.krupovic@pasteur.fr; kuhnjens@mail.nih.gov; mushegian2@gmail.com; mnibert@hms.harvard.edu; ssabanadzovic@entomology.msstate.edu; helene.sanfacon@agr.gc.ca; stuart.siddell@bristol.ac.uk; peter.simmonds@ndm.ox.ac.uk; Arvind.varsani@canterbury.ac.nz; zerbini@ufv.br; a.e.gorbalenya@lumc.nl; andrew.davison@glasgow.ac.uk RI Harrach, Balazs/A-3680-2008; Institute, Pirbright/K-4476-2014; Krupovic, Mart/I-4209-2012; Gorbalenya, Alexander/J-4818-2012; Zerbini, Francisco Murilo/N-7359-2013; OI Harrach, Balazs/0000-0002-1410-6469; Krupovic, Mart/0000-0001-5486-0098; Gorbalenya, Alexander/0000-0002-4967-7341; Zerbini, Francisco Murilo/0000-0001-8617-0200; Siddell, Stuart/0000-0002-8702-7868; Sabanadzovic, Sead/0000-0002-2995-2633 FU Medical Research Council [MC_UU_12014/3] NR 69 TC 8 Z9 8 U1 10 U2 10 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD OCT PY 2016 VL 161 IS 10 BP 2921 EP 2949 DI 10.1007/s00705-016-2977-6 PG 29 WC Virology SC Virology GA DV1LE UT WOS:000382681800036 PM 27424026 ER PT J AU Eldredge, KT Center, TD Mattison, ED AF Eldredge, K. T. Center, T. D. Mattison, E. D. TI Larval morphology and preliminary ecological observations of the uniquely phytophagous rove beetle Himalusa thailandensis (Coleoptera: Staphylinidae: Aleocharinae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID LIFE-HISTORY; NATURAL-HISTORY; PHYLOGENY AB Immature stages of the uniquely phytophagous staphylinid, Himalusa thailandensis Pace, Klimaszewski, and Center, 2010 (Coleoptera: Staphylinidae: Aleocharinae) are described. Adults chew or scrape leaf surfaces while larvae bore into leaf petioles where they mature, and then drop to the ground to pupate in silken cocoons. Compared to other aleocharines, the larval body form is unusually compact and rotund, and may be an adaptation to living within the confines of plant tissue. Mandible and maxilla morphology is unique among known larval aleocharines in being robust and dentate, and we suggest this is adaptive for scraping and squeezing plant tissue. The larval defensive gland is ribbed and gland sac ducts are kinked and apically bifid. We suggest that H. thailandensis may share this derived gland morphology with Autaliini, Bolitocharina, Gyrophaenina, Homalotina, Leptusina, and Silusina of Homalotini, and possibly Liparocephalini. The morphology and ecology detailed here for H. thailandensis is the first comprehensive description of immature stages among aleocharines where adults feed externally on plants. C1 [Eldredge, K. T.] Univ Kansas, Biodivers Inst, Div Entomol, Dept Ecol & Evolutionary Biol, 1501 Crestline Dr,Suite 140, Lawrence, KS 66045 USA. [Center, T. D.; Mattison, E. D.] ARS, Invas Plant Res Lab, USDA, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. RP Eldredge, KT (reprint author), Univ Kansas, Biodivers Inst, Div Entomol, Dept Ecol & Evolutionary Biol, 1501 Crestline Dr,Suite 140, Lawrence, KS 66045 USA. EM taroeldredge@ku.edu FU A.E.Z. Short (University of Kansas) FX The first author thanks A.E.Z. Short (University of Kansas) for financial support. The authors thank J. Parker (Columbia University) for critical review and advice on the manuscript, M. Maruyama (Kyushu University Museum) for sharing unpublished observations on related genera and helpful discussions on characters and taxonomy, J.H. Frank (University of Florida) for constructive discussion about aleocharine larvae, and last but not least, T. Thery (Universite de Montreal) for graciously providing a French translation for the abstract. NR 43 TC 0 Z9 0 U1 7 U2 7 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD OCT PY 2016 VL 148 IS 5 BP 499 EP 511 DI 10.4039/tce.2016.1 PG 13 WC Entomology SC Entomology GA DX4BQ UT WOS:000384324100001 ER PT J AU Sarvary, MA Hajek, AE Boroczky, K Raguso, RA Cooperband, MF AF Sarvary, Mark A. Hajek, Ann E. Boroczky, Katalin Raguso, Robert A. Cooperband, Miriam F. TI Investigating the effects of symbiotic fungi on the flight behaviour of Sirex noctilio (Hymenoptera: Siricidae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID WOODWASP; RESPONSES; EUROPE AB The invasive woodwasp Sirex noctilio Fabricius (Hymenoptera: Siricidae) is obligately associated with the symbiotic white rot fungus Amylostereum areolatum (Chaillet ex Fries) Boidin (Basidiomycota: Amylosteraceae), and shows positive chemotaxis to volatiles emitted by this symbiont. After introduction to North America, S. noctilio was collected carrying another fungus species Amylostereum chailletii (Persoon) Boidin, used symbiotically by native North American Sirex Linnaeus. We conducted flight behaviour studies in a walk-in flight tunnel to evaluate specificity of the attraction of mated and unmated S. noctilio to its primary symbiont, A. areolatum, versus the alternative symbiont, A. chailletii. Fewer unmated than mated S. noctilio females responded to either of the fungi. Unmated females showed no landing preference but mated S. noctilio females were attracted to A. areolatum although avoidance of A. chailletii was not complete. Chemical analysis demonstrated major differences in the volatile profiles of the two fungal species. Sesquiterpenes dominated the A. areolatum samples, whereas only two aromatic volatiles were consistently present in the native A. chailletii. C1 [Sarvary, Mark A.; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Boroczky, Katalin; Raguso, Robert A.] Cornell Univ, Dept Neurobiol & Behav, Ithaca, NY 14853 USA. [Cooperband, Miriam F.] Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol,Otis Lab, 1398W Truck Rd, Buzzards Bay, MA 02542 USA. RP Sarvary, MA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM mas245@cornell.edu FU United States Department of Agriculture-Animal and Plant Health Inspection Service FX The authors thank Sirex-wrangler S. Long, E. Morris for growing fungus; I. Caetano for mating woodwasps; and A. Anderson, K. Peters, and S. Andrews for wind tunnel trials. A. Hartness assisted in the original construction of the wind tunnel, and R. Kepler and J. Liebherr assisted with reassembly and modification. They also thank the anonymous reviewers for their thoughtful comments. Funding for this study was provided through a cooperative agreement with United States Department of Agriculture-Animal and Plant Health Inspection Service. Mention of a commercial product does not constitute an endorsement or recommendation for its use by the United States Department of Agriculture. NR 39 TC 1 Z9 1 U1 2 U2 2 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD OCT PY 2016 VL 148 IS 5 BP 543 EP 551 DI 10.4039/tce.2016.10 PG 9 WC Entomology SC Entomology GA DX4BQ UT WOS:000384324100005 ER PT J AU Turgeon, JJ Jones, C Smith, MT Orr, M Scarr, TA Gasman, B AF Turgeon, Jean J. Jones, Chuck Smith, Michael T. Orr, Mary Scarr, Taylor A. Gasman, Ben TI Records of unsuccessful attack by Anoplophora glabripennis (Coleoptera: Cerambycidae) on broadleaf trees of questionable suitability in Canada SO CANADIAN ENTOMOLOGIST LA English DT Article ID ASIAN LONGHORNED BEETLE; PERSPECTIVE; POPULATION; MANAGEMENT AB Discovery of the non-native Anoplophora glabripennis Motschulsky (Coleoptera: Cerambycidae) in Ontario, Canada, in 2003 led to the implementation of an eradication programme. The plan consisted of removing all infested trees and all trees belonging to a genus considered suitable for complete development of this wood-borer that were found within 400 m of an infested tree; however, many of the trees within that 400 m belonged to genera for which suitability for development of A. glabripennis was questionable or unknown. We visually inspected over 3000 such trees annually for the three years following removal of infested trees. All but one tree were unattacked: an ash (Fraxinus excelsior Linnaeus (Oleaceae)) tree had signs of oviposition and early-instar development, but not of adult emergence. Before that survey, we had found only one other species with questionable suitability, a little leaf linden (Tilia cordata Miller (Malvaceae)) that had many signs of oviposition, but no evidence of full development, suggesting resistance to A. glabripennis. Both of these trees were within 200 m of the most heavily infested maple (Acer platanoides Linnaeus (Sapindaceae)) tree found in that infestation, suggesting that colonisation of trees with questionable or unknown suitability might occur mostly where population pressure is high. C1 [Turgeon, Jean J.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. [Smith, Michael T.] ARS, USDA, Beneficial Insects Intro Res Lab, 501 S Chapel St, Newark, DE 19713 USA. [Orr, Mary; Gasman, Ben] Canadian Food Inspect Agcy, 1124 Finch Ave West,Unit 2, Toronto, ON M3J 2E2, Canada. [Scarr, Taylor A.] Minist Nat Resources & Forestry, 70 Foster Dr,Suite 400, Sault Ste Marie, ON P6A 6V5, Canada. RP Turgeon, JJ (reprint author), Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM jean.turgeon@canada.ca NR 39 TC 0 Z9 0 U1 9 U2 9 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD OCT PY 2016 VL 148 IS 5 BP 569 EP 578 DI 10.4039/tce.2016.2 PG 10 WC Entomology SC Entomology GA DX4BQ UT WOS:000384324100008 ER PT J AU Ingersoll, TE Sewall, BJ Amelon, SK AF Ingersoll, Thomas E. Sewall, Brent J. Amelon, Sybill K. TI Effects of white-nose syndrome on regional population patterns of 3 hibernating bat species SO CONSERVATION BIOLOGY LA English DT Article DE emerging infectious disease of wildlife; generalized additive mixed models; Myotis lucifugus; Myotis septentrionalis; Perimyotis subflavus; population monitoring; Pseudogymnoascus destructans; enfermedades infecciosas emergentes de la vida silvestre; modelos aditivos mixtos generalizados; monitoreo de poblaciones; Myotis lucifigus; Myotis septentrionalis; Perimyotis subflavus; Pseudogymnoascus destructans ID GENERALIZED ADDITIVE-MODELS; ENDANGERED INDIANA BAT; GEOMYCES-DESTRUCTANS; BROWN BATS; DISEASE; ECOLOGY; CLIMATE; SPREAD; HIBERNACULA; PERSISTENCE AB Hibernating bats have undergone severe recent declines across the eastern United States, but the cause of these regional-scale declines has not been systematically evaluated. We assessed the influence of white-nose syndrome (an emerging bat disease caused by the fungus Pseudogymnoascus destructans, formerly Geomyces destructans) on large-scale, long-term population patterns in the little brown myotis (Myotis lucifugus), the northern myotis (Myotis septentrionalis), and the tricolored bat (Perimyotis subflavus). We modeled population trajectories for each species on the basis of an extensive data set of winter hibernacula counts of more than 1 million individual bats from a 4-state region over 13 years and with data on locations of hibernacula and first detections of white-nose syndrome at each hibernaculum. We used generalized additive mixed models to determine population change relative to expectations, that is, how population trajectories differed with a colony's infection status, how trajectories differed with distance from the point of introduction of white-nose syndrome, and whether declines were concordant with first local observation of the disease. Population trajectories in all species met at least one of the 3 expectations, but none met all 3. Our results suggest, therefore, that white-nose syndrome has affected regional populations differently than was previously understood and has not been the sole cause of declines. Specifically, our results suggest that in some areas and species, threats other than white-nose syndrome are also contributing to population declines, declines linked to white-nose syndrome have spread across large geographic areas with unexpected speed, and the disease or other threats led to declines in bat populations for years prior to disease detection. Effective conservation will require further research to mitigate impacts of white-nose syndrome, renewed attention to other threats to bats, and improved surveillance efforts to ensure early detection of white-nose syndrome. Los Efectos del Si-ndrome de Nariz Blanca sobre los Patrones Regionales de Poblacin de Tres Especies de Murcielagos que Hibernan Los murcielagos que hibernan han experimentado declinaciones severas recientes a lo largo del este de los Estados Unidos pero la causa de estas declinaciones a escala regional no ha sido evaluada sistematicamente. Valoramos la influencia del sindrome de nariz blanca (una enfermedad emergente de los murcielagos causada por el hongo Pseudogymnoascus destructans, antes Geomyces destructans) en el myotis cafe (Myotis lucifugs), el myotis del norte (M. septentrionalis) y el murcielago tricolor (Perimyotis subflavus). Modelamos las trayectorias poblacionales para cada especie con base en un conjunto extenso de datos de conteos de sitios de hibernacion de mas de un millon de murcielagos individuales en una region de cuatro estados durante mas de 13 anos y con datos sobre las localidades de los sitios de hibernacion y las primeras detecciones del sindrome de nariz blanca en cada sitio de hibernacion. Usamos modelos aditivos mixtos generalizados para determinar el cambio poblacional en relacion a las expectativas, es decir, como las trayectorias poblacionales difirieron con el estado de infeccion de una colonia, como difirieron con la distancia desde el punto de introduccion del sindrome de nariz blanca y si las declinaciones fueron concordantes con la primera observacion local de la enfermedad. Las trayectorias poblacionales de todas las especies cumplieron con al menos una de las tres expectativas, pero ninguna cumplio con las tres. Nuestros resultados sugieren que el sindrome de nariz blanca ha afectado a las poblaciones regionales de maneras diferentes a como se entendia previamente y no ha sido la causa unica de las declinaciones. Especificamente, nuestros resultados sugieren que en algunas areas y en algunas especies, otras amenazas ademas del sindrome de nariz blanca tambien estan contribuyendo a las declinaciones poblacionales, que las declinaciones conectadas al sindrome de nariz blanca se han esparcido en extensas areas geograficas con una velocidad inesperada, y que la enfermedad y otras amenazas resultaron en declinaciones de la poblacion de murcielagos durante anos previos a la deteccion. La conservacion efectiva requerira de una investigacion mas profunda para mitigar los impactos del sindrome de nariz blanca, de una atencion renovada a las otras amenazas y de esfuerzos mejorados de vigilancia para asegurar la deteccion temprana del sindrome de nariz blanca. Resumen C1 [Ingersoll, Thomas E.] Univ Tennessee, Natl Inst Math & Biol Synth, Claxton Bldg,1122 Volunteer Blvd,Suite 106, Knoxville, TN 37996 USA. [Sewall, Brent J.] Temple Univ, Dept Biol, 1900 North 12th St, Philadelphia, PA 19122 USA. [Amelon, Sybill K.] Univ Missouri, US Forest Serv, Northern Res Stn, Columbia, MO 65211 USA. RP Ingersoll, TE (reprint author), Univ Tennessee, Natl Inst Math & Biol Synth, Claxton Bldg,1122 Volunteer Blvd,Suite 106, Knoxville, TN 37996 USA.; Sewall, BJ (reprint author), Temple Univ, Dept Biol, 1900 North 12th St, Philadelphia, PA 19122 USA. EM tomingersoll@yahoo.com; bjsewall@temple.edu OI Amelon, Sybill/0000-0003-3940-8153 FU National Institute for Mathematical and Biological Synthesis - National Science Foundation, Department of Homeland Security, and Department of Agriculture through NSF [EF-0832858]; University of Tennessee, Knoxville FX We thank the New York Department of Environmental Conservation, Pennsylvania Game Commission, West Virginia Department of Natural Resources, Tennessee Natural Heritage Program, and The Nature Conservancy, particularly A. Hicks, C. Butchkoski, G. Turner, C. Stihler, C. Herzog, K. O'Connor, and C. Holliday for sharing data. We also thank K. Rodriguez-Clark and 3 anonymous reviewers for comments on previous versions of this manuscript. Funding was provided to T.E.I. from the National Institute for Mathematical and Biological Synthesis (sponsored by the National Science Foundation, Department of Homeland Security, and Department of Agriculture through NSF Award EF-0832858, with support from The University of Tennessee, Knoxville). NR 55 TC 0 Z9 0 U1 83 U2 83 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD OCT PY 2016 VL 30 IS 5 BP 1048 EP 1059 DI 10.1111/cobi.12690 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DW9TX UT WOS:000384006700014 PM 26872411 ER PT J AU Brunet, J Zalapa, J Guries, R AF Brunet, Johanne Zalapa, Juan Guries, Raymond TI Conservation of genetic diversity in slippery elm (Ulmus rubra) in Wisconsin despite the devastating impact of Dutch elm disease SO CONSERVATION GENETICS LA English DT Article DE Dutch elm disease; Slippery elm; Ulmus rubra; Genetic diversity; Genetic structure; Bottleneck ID RECENT POPULATION BOTTLENECKS; ALLELE FREQUENCY DATA; MICROSATELLITE MARKERS; FOREST TREES; PUMILA ULMACEAE; PATTERNS; HYBRIDIZATION; FRAGMENTATION; LANDSCAPE; SOFTWARE AB Forest pest epidemics are responsible for many population declines reported in forest trees. While forest tree populations tend to be genetically diverse, in principle mortality resulting from disease could diminish that genetic diversity and alter the genetic structure of the remnant populations with consequences for the ability of a species to adapt to changing environments. Slippery elm (Ulmus rubra Muhl.) is a long-lived, wind-pollinated forest tree with a native range covering essentially all of eastern North America. Dutch elm disease (DED) caused by an introduced fungal pathogen (Ophiostoma ulmi) devastated North American elm populations, including slippery elm, beginning in the 1930s. Estimates of the numbers of elms lost to DED are unknown but range into the hundreds of millions of trees given their former abundance. In this study, the genotypes of 77 herbarium specimens collected between 1890 and 2004 in Wisconsin, and of 100 slippery elm trees from five wild Wisconsin populations, were characterized using 13 microsatellite loci. Levels of genetic diversity were compared between the herbarium specimens collected pre- and post-DED spread in Wisconsin. In addition, the levels of genetic diversity and degree of genetic differentiation were quantified in the five wild populations. The allelic diversity and expected levels of heterozygosity were similar between the pre- and post-DED herbarium specimens. The five wild populations were only slightly differentiated and no genetic bottleneck was detected for any population. At least in Wisconsin, slippery elm apparently has maintained levels of genetic diversity that could facilitate adaptation to future climatic and environmental changes. C1 [Brunet, Johanne] Univ Wisconsin, Dept Entomol, VCRU, USDA ARS, Madison, WI 53706 USA. [Zalapa, Juan] Univ Wisconsin, Dept Hort, USDA ARS, VCRU, Madison, WI 53706 USA. [Guries, Raymond] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Brunet, J (reprint author), Univ Wisconsin, Dept Entomol, VCRU, USDA ARS, Madison, WI 53706 USA. EM jbrunet@wisc.edu FU National Science Foundation Minority Post-doctoral Fellowship (NSF award) [0409651]; USDA-ARS FX Giovanny Covarrubias-Pazaran and Robert Phetteplace helped with figures. We gratefully acknowledge the National Science Foundation Minority Post-doctoral Fellowship (NSF award #0409651) provided to J.E. Zalapa and funding from the USDA-ARS to J. Brunet. NR 54 TC 0 Z9 0 U1 19 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD OCT PY 2016 VL 17 IS 5 BP 1001 EP 1010 DI 10.1007/s10592-016-0838-1 PG 10 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA DV4ZK UT WOS:000382934400002 ER PT J AU Vonhof, MJ Amelon, SK Currie, RR McCracken, GF AF Vonhof, Maarten J. Amelon, Sybill K. Currie, Robert R. McCracken, Gary F. TI Genetic structure of winter populations of the endangered Indiana bat (Myotis sodalis) prior to the white nose syndrome epidemic: implications for the risk of disease spread SO CONSERVATION GENETICS LA English DT Article DE Bats; Disease; Myotis sodalis; Phylogeography; Units of conservation; White nose syndrome ID MULTILOCUS GENOTYPE DATA; SEX-BIASED DISPERSAL; INFECTIOUS-DISEASE; MITOCHONDRIAL-DNA; SWARMING SITES; LANDSCAPE GENETICS; CONSERVATION UNITS; PLECOTUS-AURITUS; BECHSTEINS BAT; F-STATISTICS AB The spread of white nose syndrome raises serious concerns about the long-term viability of affected bat species. Here we examine the geographic distribution of genetic variation, levels of population connectivity that may influence the spatial spread of WNS, and the likelihood that recent population declines in regions affected by WNS have led to the loss of unique genetic variation for the endangered Indiana bat (Myotis sodalis). We amplified a fragment of the mitochondrial control region for 375 individuals and genotyped 445 individuals at 10 microsatellite loci from 18 sampling sites distributed across the majority of the species' range. Analysis of mitochondrial DNA indicated the presence of at least five distinct matrilineal clusters, with the most pronounced differences between northeastern sites and those in the rest of the range. The majority of individuals in the Ozark-Central, Midwest, and Appalachian recovery units fell into a single cluster. Significant differentiation also was observed between one Appalachian and one Midwestern site and the majority of other sites. However, using nuclear microsatellites we observed the absence of differentiation and widespread gene flow among all hibernacula, suggesting the occurrence of extensive gene flow through dispersal and mating. The absence of genetically distinct populations within the range of Indiana bats indicates a lack of barriers to WNS transmission, and it is unlikely that significant portions of the hibernating population of Indiana bats will remain disease free into the future. Further, while matrilineal gene flow was restricted among some sites and regions, we found no genetic evidence to support the division of Indiana bats into separate recovery units. C1 [Vonhof, Maarten J.] Western Michigan Univ, Dept Biol Sci, Kalamazoo, MI 49008 USA. [Vonhof, Maarten J.] Western Michigan Univ, Environm & Sustainabil Studies Program, Kalamazoo, MI 49008 USA. [Amelon, Sybill K.] Univ Missouri Columbia, US Forest Serv, Northern Res Stn, Columbia, MO USA. [Currie, Robert R.] POB 1357, Hendersonville, NC USA. [McCracken, Gary F.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN USA. RP Vonhof, MJ (reprint author), Western Michigan Univ, Dept Biol Sci, Kalamazoo, MI 49008 USA.; Vonhof, MJ (reprint author), Western Michigan Univ, Environm & Sustainabil Studies Program, Kalamazoo, MI 49008 USA. EM maarten.vonhof@wmich.edu OI Amelon, Sybill/0000-0003-3940-8153 FU US Fish and Wildlife Service; US Forest Service; University of Missouri FX This study was funded by the US Fish and Wildlife Service, the US Forest Service, and the University of Missouri. NR 106 TC 0 Z9 0 U1 36 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD OCT PY 2016 VL 17 IS 5 BP 1025 EP 1040 DI 10.1007/s10592-016-0841-6 PG 16 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA DV4ZK UT WOS:000382934400004 ER PT J AU Chapman, DJ Bishop, RC Hanemann, WM Kanninen, BJ Krosnick, JA Morey, ER Tourangeau, R AF Chapman, David J. Bishop, Richard C. Hanemann, W. Michael Kanninen, Barbara J. Krosnick, Jon A. Morey, Edward R. Tourangeau, Roger TI On the adequacy of scope test results: Comments on Desvousges, Mathews, and Train SO ECOLOGICAL ECONOMICS LA English DT Editorial Material DE Contingent valuation; Scope test; Water pollution ID CONTINGENT VALUATION AB Desrvousges et al. (2012) investigate criteria for judging the adequacy of scope test differences in contingent valuation studies. They focus particular attention on our study (Chapman et al. 2009), arguing that, while it demonstrated a statistically significant scope effect, the effect is too small. Unfortunately, DMT misinterpreted Chapman et al., an error that makes DMT's criticisms of our study invalid. (C) 2016 Elsevier B.V. All rights reserved. C1 [Chapman, David J.] Stratus Consulting, 1881 Ninth St,Suite 201, Boulder, CO 80302 USA. [Bishop, Richard C.] Univ Wisconsin, 2112 Regent St, Madison, WI 53726 USA. [Hanemann, W. Michael] Arizona State Univ, POB 875502, Tempe, AZ 85287 USA. [Hanemann, W. Michael] Univ Calif Berkeley, Dept Agr & Resource Econ, Berkeley, CA 94720 USA. [Kanninen, Barbara J.] BK Econometr LLC, Arlington, VA 22207 USA. [Krosnick, Jon A.] Stanford Univ, Polit Sci & Psychol, 434 McClatchy Hall,450 Serra Mall, Palo Alto, CA 94305 USA. [Morey, Edward R.] Univ Colorado, Dept Econ, Campus Box 256, Boulder, CO 80309 USA. [Tourangeau, Roger] Westat Corp, 1600 Res Blvd, Rockville, MD 20850 USA. [Chapman, David J.] US Forest Serv, Human Dimens Sci Program Manager, Rocky Mt Res Stn, USDA, 2150-A Ctr Ave,Bldg A 350, Ft Collins, CO 80526 USA. RP Bishop, RC (reprint author), Univ Wisconsin, 2112 Regent St, Madison, WI 53726 USA. EM rcbishop@wisc.edu; Michael.Hanemann@asu.edu; barbkann@verizon.net; krosnick@stanford.edu; Edward.Morey@Colorado.edu; RogerTourangeau@westat.com NR 4 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD OCT PY 2016 VL 130 BP 356 EP 360 DI 10.1016/j.ecolecon.2016.05.022 PG 5 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA DW8ZU UT WOS:000383944800034 ER PT J AU Okagawa, T Konnai, S Deringer, JR Ueti, MW Scoles, GA Murata, S Ohashi, K Brown, WC AF Okagawa, Tomohiro Konnai, Satoru Deringer, James R. Ueti, Massaro W. Scoles, Glen A. Murata, Shiro Ohashi, Kazuhiko Brown, Wendy C. TI Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle SO INFECTION AND IMMUNITY LA English DT Article ID BOVINE LEUKEMIA-VIRUS; OUTER-MEMBRANE PROTEINS; IN-VITRO; IMMUNE-RESPONSES; A-MARGINALE; LYMPHOCYTE; SURFACE; EXPRESSION; BLOCKADE; PERSISTENCE AB The CD4(+) T-cell response is central for the control of Anaplasma marginale infection in cattle. However, the infection induces a functional exhaustion of antigen-specific CD4(+) T cells in cattle immunized with A. marginale outer membrane proteins or purified outer membranes (OMs), which presumably facilitates the persistence of this rickettsia. In the present study, we hypothesize that T-cell exhaustion following infection is induced by the upregulation of immunoinhibitory receptors on T cells, such as programmed death 1 (PD-1) and lymphocyte activation gene 3 (LAG-3). OM-specific T-cell responses and the kinetics of PD-1-positive (PD-1(+)) LAG-3(+) exhausted T cells were monitored in A. marginale-challenged cattle previously immunized with OMs. Consistent with data from previous studies, OM-specific proliferation of peripheral blood mononuclear cells (PBMCs) and interferon gamma (IFN-gamma) production were significantly suppressed in challenged animals by 5 weeks postinfection (wpi). In addition, bacteremia and anemia also peaked in these animals at 5 wpi. Flow cytometric analysis revealed that the percentage of PD-1(+) LAG-3(+) T cells in the CD4(+), CD8(+), and delta gamma T-cell populations gradually increased and also peaked at 5 wpi. A large increase in the percentage of LAG-3(+) gamma delta T cells was also observed. Importantly, in vitro, the combined blockade of the PD-1 and LAG-3 pathways partially restored OM-specific PBMC proliferation and IFN-gamma production at 5 wpi. Taken together, these results indicate that coexpression of PD-1 and LAG-3 on T cells contributes to the rapid exhaustion of A. marginale-specific T cells following infection and that these immunoinhibitory receptors regulate T-cell responses during bovine anaplasmosis. C1 [Okagawa, Tomohiro; Konnai, Satoru; Murata, Shiro; Ohashi, Kazuhiko] Hokkaido Univ, Grad Sch Vet Med, Dept Dis Control, Sapporo, Hokkaido, Japan. [Deringer, James R.; Brown, Wendy C.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Ueti, Massaro W.; Scoles, Glen A.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. RP Brown, WC (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM wbrown@vetmed.wsu.edu FU United States Department of Agriculture-NIFA [2010-65119-20456]; USDA-ARS CRIS [5348-32000-033-00D]; USDA-ARS-CRIS [5348-32000-033-00D]; JSPS KAKENHI [25257415]; Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry, Japan [26058B]; HHS \ National Institutes of Health (NIH) [AI053692] FX This work, including the efforts of Wendy C. Brown, was funded by United States Department of Agriculture-NIFA (2010-65119-20456). This work, including the efforts of Massaro W. Ueti, was funded by USDA-ARS CRIS (5348-32000-033-00D). This work, including the efforts of Glen A. Scoles, was funded by USDA-ARS-CRIS (5348-32000-033-00D). This work, including the efforts of Satoru Konnai, was funded by JSPS KAKENHI (25257415). This work, including the efforts of Satoru Konnai, was funded by Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries, and Food Industry, Japan (26058B). This work, including the efforts of Wendy C. Brown, was funded by HHS vertical bar National Institutes of Health (NIH) (AI053692). NR 42 TC 1 Z9 1 U1 3 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD OCT PY 2016 VL 84 IS 10 BP 2779 EP 2790 DI 10.1128/IAI.00278-16 PG 12 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DX0TK UT WOS:000384077800006 PM 27430272 ER PT J AU Hammond, RW Zhang, SL AF Hammond, Rosemarie W. Zhang, Shulu TI Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE AmplifyRP (R) acceler8 (TM); Tomato; Petunia; Detection; TCDVd; Seed; Tomato chlorotic dwarf viroid; RT-RPA ID TIME RT-PCR; 1ST REPORT; GREENHOUSE TOMATOES; GEL ELECTROPHORESIS; UNITED-STATES; PLANTS; POSPIVIROIDS; SEQUENCE; DISEASE; JAPAN AB A molecular diagnostic assay utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 39 degrees C and target-specific primers and probe were developed for the rapid, sensitive, and specific detection of tomato chlorotic dwarf viroid (TCDVd) in infected leaf and seed tissues. The performance of the AmplifyRP (R) AccelerB (TM) RT-RPA diagnostic assay, utilizing a lateral flow strip contained within an amplicon detection chamber, was evaluated and the results were compared with a standard RT-PCR assay. The AmplifyRP (R) Acceler8 (TM) assay was specific for TCDVd in leaf and seed tissues, its sensitivity was comparable to conventional RT-PCR in leaf tissues, and it does not require extensive sample purification, specialized equipment, or technical expertise. This is the first report utilizing an RT-RPA assay to detect viroids and the assay can be used both in the laboratory and in the field for TCDVd detection. Published by Elsevier B.V. C1 [Hammond, Rosemarie W.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Zhang, Shulu] Agdia Inc, 52642 Cty Rd 1, Elkhart, IN 46514 USA. RP Hammond, RW (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM rose.hammond@ars.usda.gov NR 43 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD OCT PY 2016 VL 236 BP 62 EP 67 DI 10.1016/j.jviromet.2016.06.013 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DW8YR UT WOS:000383941900011 PM 27427473 ER PT J AU Shi, XJ Liu, XM Wang, Q Das, A Ma, GP Xu, L Sun, Q Peddireddi, L Jia, W Liu, YH Anderson, G Bai, JF Shi, JS AF Shi, Xiju Liu, Xuming Wang, Qin Das, Amaresh Ma, Guiping Xu, Lu Sun, Qing Peddireddi, Lalitha Jia, Wei Liu, Yanhua Anderson, Gary Bai, Jianfa Shi, Jishu TI A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Real-time PCR; RT-PCR; Diagnostic; Panel assay; Swine viruses ID POLYMERASE-CHAIN-REACTION; RESPIRATORY SYNDROME VIRUS; PORCINE CIRCOVIRUS TYPE-2; ESCHERICHIA-COLI O157; INFLUENZA-A-VIRUS; RT-PCR; FEVER VIRUS; PSEUDORABIES VIRUS; RAPID DETECTION; MOUTH-DISEASE AB Mixed infection with different pathogens is common in swine production systems especially under intensive production conditions. Quick and accurate detection and differentiation of different pathogens are necessary for epidemiological surveillance, disease management and import and export controls. In this study, we developed and validated a panel of multiplex real-time PCR/RT-PCR assays composed of four subpanels, each detects three common swine pathogens. The panel detects 12 viruses or viral serotypes, namely, VSV-IN, VSV-NJ, SVDV, CSFV, ASFV, FMDV, PCV2, PPV, PRV, PRRSV-NA, PRRSV-EU and SIV. Correlation coefficients (R-2) and PCR amplification efficiencies of all singular and triplex real-time PCR reactions are within the acceptable range. Comparison between singular and triplex real-time PCR assays of each subpanel indicates that there is no significant interference on assay sensitivities caused by multiplexing. Specificity tests on 226 target clinical samples or 4 viral strains and 91 non-target clinical samples revealed that the real-time PCR panel is 100% specific, and there is no cross amplification observed. The limit of detection of each triplex real-time PCR is less than 10 copies per reaction for DNA, and less than 16 copies per reaction for RNA viruses. The newly developed multiplex real-time PCR panel also detected different combinations of co-infections as confirmed by other means of detections. (C) 2016 Elsevier B.V. All rights reserved. C1 [Shi, Xiju; Ma, Guiping; Liu, Yanhua] Beijing Entry Exit Inspect & Quarantine Bur, Beijing, Peoples R China. [Shi, Xiju; Shi, Jishu] Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA. [Liu, Xuming; Sun, Qing; Peddireddi, Lalitha; Anderson, Gary; Bai, Jianfa] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, Manhattan, KS 66506 USA. [Wang, Qin; Xu, Lu] China Inst Vet Drug Control, Beijing, Peoples R China. [Das, Amaresh; Jia, Wei] USDA, Foreign Anim Dis Diagnost Lab, NVSL, APHIS, Greenport, NY USA. RP Shi, JS (reprint author), Kansas State Univ, Coll Vet Med, Dept Anat & Physiol, Manhattan, KS 66506 USA.; Bai, JF (reprint author), Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, Manhattan, KS 66506 USA. EM jbai@vet.ksu.edu; jshi@ksu.edu FU National Bio and Agro-Defense Facility Transition Fund [KBA-CBRI 611310]; China National Key Research and Development Programs [2016YFD0500705, 2016YFD0500901]; Kansas State Veterinary Diagnostic Laboratory revenue FX This research was supported in part by an award from the National Bio and Agro-Defense Facility Transition Fund (X. Shi and J. Shi), KBA-CBRI 611310 (J. Shi), China National Key Research and Development Programs2016YFD0500705, and 2016YFD0500901 (X. Shi), and Kansas State Veterinary Diagnostic Laboratory revenue (J. Bai). NR 32 TC 1 Z9 1 U1 15 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD OCT PY 2016 VL 236 BP 258 EP 265 DI 10.1016/j.jviromet.2016.08.005 PG 8 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DW8YR UT WOS:000383941900037 PM 27506582 ER PT J AU Porensky, LM Young, TP AF Porensky, Lauren M. Young, Truman P. TI Development of edge effects around experimental ecosystem hotspots is affected by hotspot density and matrix type SO LANDSCAPE ECOLOGY LA English DT Article DE Fragmentation; Boma; Kraal; Grazing lawn; Edge effect interactions; Multiple edges; Legacy effects; Laikipia; Ol Pejeta; Matrix quality ID ABANDONED PASTORAL SETTLEMENTS; AMAZONIAN FOREST FRAGMENTS; EAST-AFRICAN SAVANNA; LARGE HERBIVORES; LANDSCAPE HETEROGENEITY; HABITAT SELECTION; PLANT-COMMUNITIES; CERVUS-ELAPHUS; PATCH SHAPE; LONG-TERM AB Ecological edge effects are sensitive to landscape context, including matrix type and the presence of other nearby edges. In sub-Saharan Africa, temporary cattle corrals (bomas) develop into productive nutrient hotspots (glades) that attract diverse wildlife and persist for decades. Building on previous descriptive work, we experimentally altered boma configurations in an African savanna and asked how boma density and matrix type (open plains vs. bushland) influence the development of edge effects around abandoned bomas. We randomly assigned eleven plots to three treatments: one boma, two bomas 200 m apart, or two bomas 100 m apart. Before boma establishment and aeyen18 months after boma abandonment, we sampled soil nutrients, foliar nutrients, plant communities, and large herbivore use at each plot. Bomas developed into glade hotspots with elevated nutrient concentrations, altered vegetation, and elevated use by large herbivores. Few edge effects were detectable at distances aeyen50 m. Both glade density and matrix type affected the development of edge effects around experimental glades. When compared to patterns around single glades, the presence of a second glade 100 m away strengthened glade edge effects (more difference between glade and matrix), but the presence of a second glade 200 m away weakened edge effects. Vegetation edge effects were stronger in bushland areas than open plains, while wildlife shifts were strongest along the bushland-plain interface. Our results highlight the potential for edge effect variability in complex landscapes, and show that manipulative experiments can help illuminate causes and consequences of that variability. C1 [Porensky, Lauren M.; Young, Truman P.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Porensky, Lauren M.; Young, Truman P.] Mpala Res Ctr, POB 555, Nanyuki 10400, Kenya. [Porensky, Lauren M.] USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. RP Porensky, LM (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA.; Porensky, LM (reprint author), Mpala Res Ctr, POB 555, Nanyuki 10400, Kenya.; Porensky, LM (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov FU National Science Foundation (NSF) GRF; NSF DDIG [09-09539]; UC Davis Plant Sciences Departmental Fellowships; Jastro-Shields and Benjamin Madson Research Scholarships; NSF LTREB [DEB-08-16453] FX We thank Ol Pejeta Conservancy for the opportunity to conduct this research. Fredrick Erii, John Lochukuya, Mathew Namoni, Jackson Ekadeli, James Wambogo, Wilson Murithi, Solveig Franziska Bucher, Michael Ellis, John Porensky, Johnathon Choge, Solomon Kipngeno Mutai, and Robert Osusu provided invaluable field assistance. We thank Giles Prettejohn, Nathan Gichohi, Samuel Mutisya, Joseph Mathenge, Batian Craig, Martin Mulama, Douglas Kamaru, Irene Anyango, and Caroline Ngweno for advice, assistance with experimental setup, and logistical support. We received helpful advice on experimental design and comments on the manuscript from V. Eviner, M. L. Cadenasso, K. E. Veblen, and five anonymous reviewers. This research complies with Kenyan law and was approved by the Kenyan Ministry of Science and Technology. Financial support came from National Science Foundation (NSF) GRF, NSF DDIG (09-09539), and UC Davis Plant Sciences Departmental Fellowships, Jastro-Shields and Benjamin Madson Research Scholarships (to LEM), and NSF LTREB DEB-08-16453 (to TPY). NR 65 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD OCT PY 2016 VL 31 IS 8 BP 1663 EP 1680 DI 10.1007/s10980-016-0344-3 PG 18 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DV4PD UT WOS:000382906600003 ER PT J AU Cruz, CD Magarey, RD Christie, DN Fowler, GA Fernandes, JM Bockus, WW Valent, B Stack, JP AF Cruz, Christian D. Magarey, Roger D. Christie, David N. Fowler, Glenn A. Fernandes, Jose M. Bockus, William W. Valent, Barbara Stack, James P. TI Climate Suitability for Magnaporthe oryzae Triticum Pathotype in the United States SO PLANT DISEASE LA English DT Article ID GRAY LEAF-SPOT; PERENNIAL RYEGRASS TURF; PYRICULARIA-GRISEA; INTEGRATED PEST; PLANT-PATHOGENS; RICE BLAST; RISK MAPS; WHEAT; SYSTEM; DISEASE AB Wheat blast, caused by the Triticum pathotype of Magnaporthe oryzae, is an emerging disease considered to be a limiting factor to wheat production in various countries. Given the importance of wheat blast as a high-consequence plant disease, weather-based infection models were used to estimate the probabilities of M. oryzae Triticum establishment and wheat blast outbreaks in the United States. The models identified significant disease risk in some areas. With the threshold levels used, the models predicted that the climate was adequate for maintaining M. oryzae Triticum populations in 40% of winter wheat production areas of the United States. Disease outbreak threshold levels were only reached in 25% of the country. In Louisiana, Mississippi, and Florida, the probability of years suitable for outbreaks was greater than 70%. The models generated in this study should provide the foundation for more advanced models in the future, and the results reported could be used to prioritize research efforts regarding the biology of M. oryzae Triticum and the epidemiology of the wheat blast disease. C1 [Cruz, Christian D.; Bockus, William W.; Valent, Barbara; Stack, James P.] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Magarey, Roger D.; Christie, David N.] North Carolina State Univ, Ctr IPM, Raleigh, NC 27606 USA. [Fowler, Glenn A.] Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol,Plant Epidemiol & Ri, Raleigh, NC 27606 USA. [Fernandes, Jose M.] Embrapa Trigo, BR-99001970 Passo Fundo, RS, Brazil. RP Stack, JP (reprint author), Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. EM jstack@ksu.edu FU Agriculture and Food Research Initiative from the United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) [2009-55605-05201, 2013-68004-20378]; Animal and Plant Health Inspection Service Plant Protection and Quarantine; USDA-NIFA AFRI [2015-68004-23179] FX The project has been funded by Agriculture and Food Research Initiative Competitive Grants 2009-55605-05201 and 2013-68004-20378 from the United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA). Contribution number 15-438-J from the Kansas Agricultural Experiment Station. NAPPFAST was formerly supported by the Animal and Plant Health Inspection Service Plant Protection and Quarantine. The work was also supported by the USDA-NIFA AFRI Competitive Grants Program Food Security Challenge Area grant 2015-68004-23179. NR 65 TC 0 Z9 0 U1 12 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 1979 EP 1987 DI 10.1094/PDIS-09-15-1006-RE PG 9 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500002 ER PT J AU Goncalves-Zuliani, AMO Nanami, DSY Barbieri, BR Guedes, TA Zanutto, CA Bock, CH Nunes, WMC AF Goncalves-Zuliani, Aline M. O. Nanami, Danielle S. Y. Barbieri, Bruna R. Guedes, Terezinha A. Zanutto, Carlos A. Bock, Clive H. Nunes, William M. C. TI Evaluation of Resistance to Asiatic Citrus Canker among Selections of Pera Sweet Orange (Citrus sinensis) SO PLANT DISEASE LA English DT Article ID AXONOPODIS PV. CITRI; XANTHOMONAS-CAMPESTRIS; BACTERIAL SPOT; FLORIDA; STRAINS; SEVERITY; DISEASES; AGGRESSIVENESS; SUSCEPTIBILITY; INOCULATION AB Asiatic citrus canker (ACC, caused by the bacterium Xanthonionas citri subsp. citri) is a destructive disease of citrus in Brazil and in several other citrus-producing countries. ACC management is problematic, and bactericides such as copper can be reasonably efficacious but do not completely control the disease. Furthermore, injury by citrus leaf miner (CLM) can exacerbate severity of ACC. Host resistance is the most desirable solution for management of ACC; however, evaluations of germplasm indicate that resistance is limited in many popular species and cultivars that are grown commercially. Limited evaluations have been made of sweet orange (Citrus sinensis) selections. We evaluated resistance of 25 Pera sweet orange selections to X citri subsp. citri by wound inoculation and measuring lesion diameter under greenhouse conditions (wound inoculation indicates mesophyll resistance which will be valuable in areas where CLM exists). ACC severity was assessed on the same 25 selections at three locations in the field in Brazil, relying on natural inoculum and conditions to cause disease. In the greenhouse experiments, the selections EEL, Bianchi/CC, Ipigua, Olimpia, IAC 2000/1, and Ovale Siracusa consistently had the smallest diameter lesions, indicating greatest resistance, although differences in lesion diameter were small. Results from the field experiments were less conclusive, although EEL and Ovale Siracusa were consistently numerically least affected by ACC. These results indicate selections of sweet orange that might be preferable to consider in canker-prone areas in Brazil and elsewhere. C1 [Goncalves-Zuliani, Aline M. O.; Nanami, Danielle S. Y.; Barbieri, Bruna R.; Zanutto, Carlos A.; Nunes, William M. C.] Univ Estadual Maringa, Nucleo Pesquisa Biotecnol Aplicada, BR-87020900 Maringa, Parana, Brazil. [Guedes, Terezinha A.] Univ Estadual Maringa, Dept Estat, BR-87020900 Maringa, Parana, Brazil. [Bock, Clive H.] ARS, USDA, Southeastern Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Nunes, WMC (reprint author), Univ Estadual Maringa, Nucleo Pesquisa Biotecnol Aplicada, BR-87020900 Maringa, Parana, Brazil. EM wmcnunes@uem.br FU National Council for Scientific and Technological Development; Coordination of Improvement of Higher Education Personnel FX We thank The National Council for Scientific and Technological Development for financial aid provided and granted to W. M. C. Nunes, Coordination of Improvement of Higher Education Personnel for financial assistance granted to A. M. O. Goncalves-Zuliani and D. S. Y. Nanami through a scholarship, J. A. Remolli for substantial assistance in plant maintenance and data collection, and J. Belasque, Jr. for help with the evaluation method of greenhouse plants. NR 42 TC 0 Z9 0 U1 6 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 1994 EP 2000 DI 10.1094/PDIS-04-16-0502-RE PG 7 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500004 ER PT J AU Shishkoff, N Camp, MJ AF Shishkoff, Nina Camp, Mary J. TI The Effect of Different Temperatures and Moisture Levels on Survival of Calonectria pseudonaviculata in Boxwood Leaves and Twigs and as Microsclerotia Produced in Culture SO PLANT DISEASE LA English DT Article ID CYLINDROCLADIUM-CROTALARIAE MICROSCLEROTIA; 1ST REPORT; SOIL; ROOT; GERMINABILITY; RESISTANCE; BUXICOLA AB Leaves and twig sections of boxwood infected with Calonectria pseudonaviculata were incubated in sand at two moisture levels (36% [carrying capacity] and 5% water [vol/vol]) and at five temperatures (-10, 0, 10, 20, and 30 degrees C). Percent sporulation from monthly tissue samples plated on glucose yeast-extract tyrosine media declined to zero after 5 months at 30 degrees C and after 7 months at 10 degrees C. At 0, 10, and 20 degrees C, sporulation was observed through 30 months. Statistical analysis of data collected over 16 months of sampling showed a significant effect for temperature in all sample types, with maximum survival at 10 degrees C. For discrete microsclerotia grown on cellophane sheets, sporulation was not observed after 2 months at 30 degrees C or after 7 months at -0 degrees C. At all other temperatures, they continued to germinate over the 30 months of sampling. Statistical analysis showed significant effects for temperature and moisture level, with maximum survival at 0 degrees C in moist soil over 16 months. These results suggest that extremes of heat and cold will kill the pathogen in plant debris but, at moderate temperatures, it will remain in soil for long periods, making replanting in affected sites in well-moderated climates difficult. C1 [Shishkoff, Nina] USDA ARS, Foreign Dis Weed Sci Res Unit, Frederick, MD 21771 USA. [Camp, Mary J.] USDA ARS, Northeast Area Stat Grp, Beltsville, MD 20705 USA. RP Shishkoff, N (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, Frederick, MD 21771 USA. EM nina.shishkoff@ars.usda.gov FU Farm Bill project through the Animal and Plant Health Inspection Service FX We thank F. Eskandari and K. Johnson for technical assistance. Funding support was provided from the 2012-2013 Farm Bill project through the Animal and Plant Health Inspection Service. NR 28 TC 0 Z9 0 U1 6 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 2018 EP 2024 DI 10.1094/PDIS-09-15-1098-RE PG 7 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500007 ER PT J AU Uppala, SS Wu, BM Alderman, SC AF Uppala, Sai Sree Wu, B. M. Alderman, S. C. TI Effects of Temperature and Duration of Preconditioning Cold Treatment on Sclerotial Germination of Claviceps purpurea SO PLANT DISEASE LA English DT Article ID MALE-STERILE BARLEY; KENTUCKY BLUEGRASS; ERGOT; SEED; OREGON; SUSCEPTIBILITY; FUNGICIDES; RESISTANCE; SORGHUM AB Claviceps purpurea is an important ovary-infecting pathogen that replaces seed with sclerotia in Kentucky bluegrass grown for seed. Sclerotia over winter in the soil and geminate in the spring to produce ascospores that infect grass seed ovaries. To better understand environmental conditions affecting ascospore production, the effects of preconditioning cold treatment and subsequent incubation temperature on germination of sclerotia were determined in growth chambers under controlled conditions. Preconditioning cold treatment was essential for germination only in treatments where the incubation temperature was high (at least higher than 20 degrees C). At lower incubation temperatures (10 to 20 degrees C), preconditioning also played a role in improving sclerotial germination. Preconditioning at 4 degrees C (in darkness) for 4 to 8 weeks followed by incubation at 10 and 20 degrees C (cycle of 12 h each of darkness and light), or constant 15 degrees C (cycle of 12 h each of darkness and light), was optimal for ergot germination. When sclerotia were preconditioned for 4 weeks or longer, number of incubation days required for initiation of germination was not affected by temperature in the range from 10 to 25 degrees C (cycle of 12 h each of darkness and light), although the duration of germination (or the progress speed of germination) was still affected by temperature. A simple model was developed based on laboratory results and validated with historic spore trap data collected from various Kentucky bluegrass fields in Oregon (Willamette Valley, central Oregon, and Grande Ronde Valley). The prediction model could predict ascospore onset well and explained 55% of variation in the data. C1 [Uppala, Sai Sree] Cent Oregon Agr Res Ctr, Madras, OR 97741 USA. [Wu, B. M.] China Agr Univ, Dept Plant Pathol, Beijing, Peoples R China. [Alderman, S. C.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Wu, BM (reprint author), China Agr Univ, Dept Plant Pathol, Beijing, Peoples R China. EM bmwu@cau.edu.cn FU Agricultural Research Foundation, Oregon State University FX This study was funded by the Agricultural Research Foundation, Oregon State University. NR 41 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 2080 EP 2086 DI 10.1094/PDIS-02-16-0215-RE PG 7 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500015 ER PT J AU Saito, S Michailides, TJ Xiao, CL AF Saito, S. Michailides, T. J. Xiao, C. L. TI Fungicide Resistance Profiling in Botrytis cinerea Populations from Blueberry in California and Washington and Their Impact on Control of Gray Mold SO PLANT DISEASE LA English DT Article ID CYTOCHROME-B GENE; CONTROLLED-ATMOSPHERE STORAGE; BASE-LINE SENSITIVITY; MOLECULAR CHARACTERIZATION; HIGHBUSH BLUEBERRIES; VEGETABLE CROPS; FRUIT; PYRACLOSTROBIN; STRAWBERRY; APPLE AB Gray mold caused by Botrytis cinerea is a major postharvest disease of blueberry grown in the Central Valley of California and western Washington State. Sensitivities to boscalid, cyprodinil, fenhexamid, fludioxonil, and pyraclostrobin, representing five different fungicide classes, were examined for 249 (California) and 106 (Washington) B. cinerea isolates recovered from decayed blueberry fruit or flowers. In California and Washington, 7 and 17 fungicide-resistant phenotypes, respectively, were detected: 66 and 49% of the isolates were resistant to boscalid, 20 and 29% were moderately resistant to cyprodinil, 29 and 29% were resistant to fenhexamid, and 66 and 55% were resistant to pyraclostrobin. All isolates from California were sensitive to fludioxonil, whereas 70% of the isolates from Washington showed reduced sensitivity to fludioxonil. In California, 26 and 30% of the isolates were resistant to two and three classes of fungicides, respectively. In Washington, 31, 14, 16, and 9% of the isolates were resistant to two, three, four, and five classes of fungicides, respectively. Inherent risk of the development of resistance to quinone outside inhibitor (QoI) fungicides was assessed by detecting the presence of the Bcbi-143/144 intron in gene cytb. The intron was detected in 11.8 and 40% of the isolates in California and Washington, respectively, suggesting that the risk of QoI resistance is higher in California than in Washington. On detached blueberry fruit inoculated with 11 isolates exhibiting different fungicide-resistant phenotypes, most fungicides failed to control gray mold on fruit inoculated with the respective resistant phenotypes but the mixture of cyprodinil and fludioxonil was effective against all fungicide-resistant phenotypes tested. Our findings would be useful in designing and implementing fungicide resistance management spray programs for control of gray mold in blueberry. C1 [Saito, S.; Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Michailides, T. J.] Univ Calif, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov NR 50 TC 0 Z9 0 U1 14 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 2087 EP 2093 DI 10.1094/PDIS-02-16-0229-RE PG 7 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500016 ER PT J AU Rivera, Y Stonunel, J Dumm, J Ismaiel, A Wyenandt, CA Crouch, JA AF Rivera, Y. Stonunel, J. Dumm, J. Ismaiel, A. Wyenandt, C. A. Crouch, J. A. TI First Report of Colletotrichum nigrum Causing Anthracnose Disease on Tomato Fruit in New Jersey SO PLANT DISEASE LA English DT News Item C1 [Rivera, Y.; Ismaiel, A.; Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Rivera, Y.; Wyenandt, C. A.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Stonunel, J.; Dumm, J.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. RP Rivera, Y (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA.; Rivera, Y (reprint author), Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. OI Crouch, Jo Anne/0000-0001-6886-8090 NR 3 TC 0 Z9 0 U1 4 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 2162 EP 2162 DI 10.1094/PDIS-02-16-0174-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500036 ER PT J AU Hansen, MA Demers, J Sutphin, M Yoder, K Bush, E Castlebury, L AF Hansen, M. A. Demers, J. Sutphin, M. Yoder, K. Bush, E. Castlebury, L. TI First Report of European Pear Rust (Pear Trellis Rust) Caused by Gymnosporangium sabinae on Ornamental Pear (Pyrus calleryana) in Virginia SO PLANT DISEASE LA English DT News Item C1 [Hansen, M. A.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Demers, J.; Castlebury, L.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Sutphin, M.] Virginia Cooperat Extens, Winchester, VA USA. [Yoder, K.; Bush, E.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. RP Hansen, MA (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. NR 3 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD OCT PY 2016 VL 100 IS 10 BP 2166 EP 2166 DI 10.1094/PDIS-03-16-0396-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DW4VG UT WOS:000383640500047 ER PT J AU Baehr, A Holland, T Biala, K Margolis, GS Wiebe, DJ Carr, BG AF Baehr, Avi Holland, Tara Biala, Karen Margolis, Gregg S. Wiebe, Douglas J. Carr, Brendan G. TI Describing Total Population Health: A Review and Critique of Existing Units SO POPULATION HEALTH MANAGEMENT LA English DT Review ID CARE COALITIONS; STATES; PREPAREDNESS; HOSPITALS; SERVICES; ACCESS; STROKE AB Total population health is a key tenet of health care reform efforts, evident in initiatives such as the National Quality Strategy, shifts toward population-based payments, and community benefit requirements for tax-exempt hospitals. Representing total population health in a way that guides best practices and establishes shared accountability for geographic communities, however, remains a challenge in part because of differences in how stakeholders define populations. To better understand the landscape of potential denominators for population health, this study examined a selection of relevant geographic units. The approach included a comprehensive review of health services and public health research literature as well as recent pertinent health policy documents. Units were characterized based on whether they: exhibit "breadth" of coverage across the whole US population; are "accurate" or grounded in health care utilization patterns; are "actionable" with mechanisms for implementing funding and regulation; and promote "synergism" or effective coordination of public health and health care activities. Although other key components of a total population health unit may exist and no single identified unit possesses all of the aforementioned features, several promising candidates were identified. Specifically, healthcare coalitions link health care and public health domains to care for a geographic community, but their connection to utilization is not empiric and limited funding exists at the coalition level. Although Accountable Care Organizations do not uniformly incorporate public health or facilitate coordination across all payers or providers, they represent an effective mechanism to increase collaboration within health care systems and represent a potential building block to influence total population health. C1 [Baehr, Avi] US Dept HHS, Off Dis Prevent & Hlth Promot, Rockville, MD USA. [Baehr, Avi; Wiebe, Douglas J.] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA. [Holland, Tara] GAP Solut Inc, Washington, DC USA. [Biala, Karen; Margolis, Gregg S.; Carr, Brendan G.] US Dept HHS, Emergency Care Coordinat Ctr, Off Assistant Secretary Preparedness & Response, Washington, DC 20201 USA. [Biala, Karen] Food & Nutr Serv, USDA, Alexandria, VA USA. [Wiebe, Douglas J.] Univ Penn, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Carr, Brendan G.] Thomas Jefferson Univ, Sidney Kimmel Med Coll, Philadelphia, PA 19107 USA. RP Baehr, A (reprint author), Jordan Med Educ Ctr, Perelman Sch Med, 6th Floor,3400 Civ Ctr Blvd,Bldg 421, Philadelphia, PA 19104 USA. EM avi.baehr@gmail.com NR 35 TC 1 Z9 1 U1 5 U2 5 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1942-7891 EI 1942-7905 J9 POPUL HEALTH MANAG JI Popul. Health Manag. PD OCT PY 2016 VL 19 IS 5 BP 306 EP 314 DI 10.1089/pop.2015.0105 PG 9 WC Health Care Sciences & Services SC Health Care Sciences & Services GA DW9VB UT WOS:000384009700003 PM 26824267 ER PT J AU Hoy, MK Goldman, JD Sebastian, RS AF Hoy, M. Katherine Goldman, Joseph D. Sebastian, Rhonda S. TI Fruit and vegetable intake of US adults estimated by two methods: What We Eat In America, National Health and Nutrition Examination Survey 2009-2012 SO PUBLIC HEALTH NUTRITION LA English DT Article DE Fruit and vegetable intake; Adults; What We Eat in America; National Health and Nutrition Examination Survey; Dietary Guidelines for Americans ID CONSUMPTION; ISSUES AB Objective: Estimates of fruit and vegetable (FV) consumption vary depending on intake definition, which may be determined by research purpose. Researchers have used two methods to evaluate intake: epidemiological and behavioural. The present study describes FV intake by adults using epidemiological v. behavioural approaches. Design: One-day dietary intake data from What We Eat In America, National Health and Nutrition Examination Survey 2009-2012 were used. Sample weights were used to produce nationally representative estimates. FV intake (in cupequivalents (CE)) was estimated using the Food Patterns Equivalents Database. The epidemiological method considered all FV after disaggregating foods and beverages. The behavioural method included foods that provided at least 0.2 CE FV per 100 g, and excluded sources high in fat, added sugar and Na. Setting: Nationally representative survey of the US population. Subjects: Adults (n 10 563) aged >= 20 years. Results: For epidemiological v. behavioural, fruit intake was 1.1 v. 1.0 CE for males and 1.0 v. 0.9 CE for females. Vegetable intake was 1.8 v. 1.1 CE for males and 1.5 v. 1.0 CE for females. Conclusions: The definition of FV intake affects estimates of consumption by the population and is an important consideration when planning and comparing research studies. The method used should align with research goals to assure accurate interpretation and validity of results. C1 [Hoy, M. Katherine; Goldman, Joseph D.; Sebastian, Rhonda S.] ARS, USDA, Food Surveys Res Grp, Beltsville Human Nutr Res Ctr, 10300 Baltimore Ave Bldg 005, Beltsville, MD 20705 USA. RP Hoy, MK (reprint author), ARS, USDA, Food Surveys Res Grp, Beltsville Human Nutr Res Ctr, 10300 Baltimore Ave Bldg 005, Beltsville, MD 20705 USA. EM Kathy.hoy@ars.usda.gov NR 16 TC 1 Z9 1 U1 10 U2 10 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD OCT PY 2016 VL 19 IS 14 BP 2508 EP 2512 DI 10.1017/S1368980016000628 PG 5 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA DX5IP UT WOS:000384414900003 PM 27029618 ER PT J AU Mascarin, GM Jackson, MA Behle, RW Kobori, NN Delalibera, I AF Mascarin, Gabriel Moura Jackson, Mark A. Behle, Robert W. Kobori, Nilce N. Delalibera Junior, Italo TI Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Mycoinsecticides; Liquid fermentation; Air drying; Spray drying; Storage stability; Oxygen scavengers; Desiccants ID FUNGUS PAECILOMYCES-FUMOSOROSEUS; ENTOMOPATHOGENIC FUNGI; DESICCATION-TOLERANCE; LIQUID CULTURES; CONIDIA; TEMPERATURES; MEDIA; FERMENTATION; SURVIVAL; OXYGEN AB The yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to < 5 % water content equivalent to a(w) < 0.3. The viability of B. bassiana blastospores after air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 A degrees C compared with 28 A degrees C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 A degrees C, viability was significantly prolonged for both air- and spray-dried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (similar to twofold higher half-life) when stored at 28 A degrees C. After storage for 4 months at 28 A degrees C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low a(w) and oxygen availability) that improved blastospore storage stability at 28 A degrees C and will facilitate the commercial development of blastospores-based bioinsecticides. C1 [Mascarin, Gabriel Moura] Embrapa Arroz & Feijao, Rodovia GO 462,Km 12,Zona Rural,CP 179, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Mascarin, Gabriel Moura; Jackson, Mark A.; Behle, Robert W.; Kobori, Nilce N.] ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Mascarin, Gabriel Moura; Delalibera Junior, Italo] Univ Sao Paulo ESALQ USP, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11,CP 9, BR-13418900 Piracicaba, SP, Brazil. RP Mascarin, GM (reprint author), Embrapa Arroz & Feijao, Rodovia GO 462,Km 12,Zona Rural,CP 179, BR-75375000 Santo Antonio De Goias, Go, Brazil.; Mascarin, GM; Jackson, MA (reprint author), ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA.; Mascarin, GM (reprint author), Univ Sao Paulo ESALQ USP, Dept Entomol & Acarol, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11,CP 9, BR-13418900 Piracicaba, SP, Brazil. EM gabriel.mascarin@embrapa.br; mark.jackson@ars.usda.gov RI Mascarin, Gabriel/E-4646-2015 OI Mascarin, Gabriel/0000-0003-3517-6826 FU EMBRAPA (Empresa Brasileira de Pesquisa Agropecuaria) FX We thank EMBRAPA (Empresa Brasileira de Pesquisa Agropecuaria) for the scholarship to the first author. The technical support provided by Angela Payne (USDA, Peoria-IL) throughout these studies is much appreciated. NR 38 TC 1 Z9 1 U1 9 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD OCT PY 2016 VL 100 IS 19 BP 8359 EP 8370 DI 10.1007/s00253-016-7597-2 PG 12 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DV9EI UT WOS:000383242500009 PM 27198727 ER PT J AU Webster, CD Rawles, SD Koch, JF Thompson, KR Kobayashi, Y Gannam, AL Twibell, RG Hyde, NM AF Webster, C. D. Rawles, S. D. Koch, J. F. Thompson, K. R. Kobayashi, Y. Gannam, A. L. Twibell, R. G. Hyde, N. M. TI Bio-Ag reutilization of distiller's dried grains with solubles (DDGS) as a substrate for black soldier fly larvae, Hermetia illucens, along with poultry by-product meal and soybean meal, as total replacement of fish meal in diets for Nile tilapia, Oreochromis niloticus SO AQUACULTURE NUTRITION LA English DT Article DE distiller's dried grains with solubles; poultry by-product meal; Nile tilapia; Oreochromis niloticus ID X MORONE-SAXATILIS; TROUT ONCORHYNCHUS-MYKISS; CRAYFISH CHERAX-QUADRICARINATUS; AMINO-ACID AVAILABILITY; FED PRACTICAL DIETS; CHANNEL CATFISH; PROTEIN-SOURCES; RAINBOW-TROUT; ICTALURUS-PUNCTATUS; FEED INGREDIENTS AB A feeding trial was conducted in a closed system with Nile tilapia, Oreochromis niloticus, juveniles (mean initial weight, 2.66 g) to examine total replacement of menhaden fish meal (FM) with distiller's dried grains with solubles (DDGS), which had been used as substrate for the production of black soldier fly larvae, Hermetia illucens, in combination with soybean meal (SBM) and poultry by-product meal (PBM), with or without supplementation of the amino acids (AA) DL-methionine (Met), L-lysine (Lys) and a commercial non-amylaceous polysaccharide enzyme (Enz) product. Fish were fed seven isoenergetic [available energy (AE) = 4.0 kcal g(-1) of diet] and isonitrogenous (350 g kg(-1) protein as-fed basis) practical diets formulated with equivalent digestible protein levels. Diet 1 was formulated to be similar to a commercial, high-quality, tilapia diet containing 200 g kg(-1) FM. Diets 2-5 were formulated as a 2 9 2 factorial to replace FM with similar contributions from DDGS (45%), PBM (25%) and SBM (2.1-2.9%), but to differ in supplementation of AA and/or Enz preparation. Diets 6 and 7 were formulated to investigate the effects of a 2/3 and 1/3 reduction, respectively, in DDGS contribution to the replacement protein mix, with concomitant increases in SBM, with respect to diet 3, and were balanced with Lys and Met. After 6 weeks, growth responses were slightly attenuated (P <= 0.05) and average daily intake (ADI) and feed conversion ratio (FCR) were slightly higher in tilapia fed DDGS diets 2-5 compared to those of fish fed the FM control diet 1. Growth responses were not significantly affected by the presence or absence of AA or Enz (diets 2-5), or the level of DDGS (diets 3, 7 and 6). Whole-body proximate composition was not different among treatments. Amino acid profiles of fish fed DDGS diets were not significantly different from those of fish fed the FM control. Evidence of interaction between AA and Enz supplementation was detected in whole-body amino acid concentrations such that AA content was higher with AA or Enz addition alone, but lower when both were added to the diet. Results suggest that DDGS replacement of FM in tilapia diets can be substantial when diets are formulated on a digestible protein basis and DDGS is combined with highly digestible animal (PBM) and plant proteins (SBM). C1 [Webster, C. D.; Rawles, S. D.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. [Koch, J. F.] Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil. [Thompson, K. R.; Kobayashi, Y.] Kentucky State Univ, Aquaculture Res Ctr, Frankfort, KY USA. [Gannam, A. L.; Twibell, R. G.; Hyde, N. M.] US Fish & Wildlife Serv, Abernathy Fish Technol Ctr, Longview, WA USA. RP Webster, CD (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM carl.webster@ars.usda.gov FU Kentucky Soybean Board; USDA 1890 Institution Capacity Building Grant; USDA [KYX-80-09-18A] FX The authors thank N. Ann, K.C., K.N. Dee, B.R. Lee, E.M. Maa, Cathy Rhin, B. Rett, M.S. Tee, D.R. Wynne and Sam Wise for technical assistance; this research project was partially funded by a grant from the Kentucky Soybean Board, a USDA 1890 Institution Capacity Building Grant and a USDA grant under agreement KYX-80-09-18A to Kentucky State University. NR 53 TC 0 Z9 0 U1 27 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD OCT PY 2016 VL 22 IS 5 BP 976 EP 988 DI 10.1111/anu.12316 PG 13 WC Fisheries SC Fisheries GA DW0RV UT WOS:000383350500004 ER PT J AU Kimball, BA AF Kimball, Bruce A. TI Volatile metabolome: problems and prospects SO BIOANALYSIS LA English DT Editorial Material DE biomarkers; disease; microbiome; odor ID MASS-SPECTROMETRY; QUANTIFICATION; MICROBIOME; BEHAVIOR C1 [Kimball, Bruce A.] Monell Chem Senses Ctr, USDA APHIS WS NWRC, 3500 Market St, Philadelphia, PA 19104 USA. RP Kimball, BA (reprint author), Monell Chem Senses Ctr, USDA APHIS WS NWRC, 3500 Market St, Philadelphia, PA 19104 USA. EM bruce.a.kimball@aphis.usda.gov NR 22 TC 0 Z9 0 U1 6 U2 6 PU FUTURE SCI LTD PI LONDON PA UNITED HOUSE, 2 ALBERT PL, LONDON, N3 1QB, ENGLAND SN 1757-6180 EI 1757-6199 J9 BIOANALYSIS JI Bioanalysis PD OCT PY 2016 VL 8 IS 19 BP 1987 EP 1991 DI 10.4155/bio-2016-0203 PG 5 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA DW2XO UT WOS:000383505800001 PM 27532599 ER PT J AU Brent, CS Penick, CA Trobaugh, B Moore, D Liebig, J AF Brent, Colin S. Penick, Clint A. Trobaugh, Beth Moore, Dani Liebig, Jurgen TI Induction of a reproductive-specific cuticular hydrocarbon profile by a juvenile hormone analog in the termite Zootermopsis nevadensis SO CHEMOECOLOGY LA English DT Article DE Cuticular hydrocarbons; Fertility signaling; Gonads; Juvenile hormone; Termites ID ANGUSTICOLLIS HAGEN ISOPTERA; DAMP-WOOD TERMITE; RETICULITERMES-FLAVIPES; CASTE DIFFERENTIATION; DIPLOPTERA-PUNCTATA; BLATTELLA-GERMANICA; SECONDARY REPRODUCTIVES; NEOTENIC REPRODUCTIVES; OVARIAN MATURATION; QUEEN PHEROMONES AB Establishment and maintenance of the reproductive division of labor within social insect colonies relies on clear communication between nestmates. Fertile members convey their status to prevent others from becoming reproductively active. Recent findings in some basal termites indicate that cuticular hydrocarbon profiles may indicate reproductive state, but there is little evidence to show a direct link between reproductive status and hydrocarbon production-a prerequisite for an "honest" fertility signal. Here, we report that the putative signaling mechanism is influenced by juvenile hormone (JH), a primary regulator of gonadal development and activity in insects. Topical application of a JH-analog (pyriproxyfen) to reproductively inactive alates of the basal dampwood termite Zootermopsis nevadensis induced both females and males to express significantly more of a reproductive-specific hydrocarbon (6,9,17-tritriacontatriene). However, the JH-analog did not significantly enhance gonadal development or activity in treated termites beyond what is usually observed in maturing alates released from the inhibitory stimuli of their natal nest. These results suggest that a rise in JH following disinhibition drives the expression of reproductive-specific hydrocarbons, but that an individual's hydrocarbon profile is not directly linked to its gonadal state. Rather than directly driving the expression of reproductive-specific hydrocarbons, the gonads may act indirectly through their influence on circulating JH. C1 [Brent, Colin S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Penick, Clint A.; Trobaugh, Beth; Moore, Dani; Liebig, Jurgen] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Penick, Clint A.] North Carolina State Univ, Dept Biol, Raleigh, NC 27695 USA. RP Brent, CS (reprint author), USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM colin.brent@ars.usda.gov OI Brent, Colin/0000-0003-2078-1417 FU Agriculture and Food Research Initiative Competitive Grant from the United States Department of Agriculture National Institute of Food and Agriculture [2007-35302-18172] FX We thank the administrators of the Pebble Beach Company for permission to collect termites. We would also like to thank Kevin Haight for assisting with colony collection and maintenance, and Steven Prager for assisting with colony maintenance and JHA treatments. All experiments were conducted in accordance with American statutes governing research. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This project was supported by the Agriculture and Food Research Initiative Competitive Grant No. 2007-35302-18172 from the United States Department of Agriculture National Institute of Food and Agriculture. NR 92 TC 0 Z9 0 U1 46 U2 46 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0937-7409 EI 1423-0445 J9 CHEMOECOLOGY JI Chemoecology PD OCT PY 2016 VL 26 IS 5 BP 195 EP 203 DI 10.1007/s00049-016-0219-8 PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DV7HF UT WOS:000383106300004 ER PT J AU Sato, K Okajima, F Miyashita, K Imamura, S Kobayashi, J Stanhope, KL Havel, PJ Machida, T Sumino, H Murakami, M Schaefer, E Nakajima, K AF Sato, Koichi Okajima, Fumikazu Miyashita, Kazuya Imamura, Shigeyuki Kobayashi, Junji Stanhope, Kimber L. Havel, Peter J. Machida, Tetsuo Sumino, Hiroyuki Murakami, Masami Schaefer, Ernst Nakajima, Katsuyuki TI The majority of lipoprotein lipase in plasma is bound to remnant lipoproteins: A new definition of remnant lipoproteins SO CLINICA CHIMICA ACTA LA English DT Article DE Lipoprotein lipase (LPL); Tetrahydrolipstatin (THL); Chylomicron remnants (CM-R); Very low density lipoprotein remnants (VLDL-R); Remnant-like lipoprotein particles-cholesterol (RLP-C) ID TRIGLYCERIDE-RICH LIPOPROTEINS; RECEPTOR-RELATED PROTEIN; LOW-DENSITY LIPOPROTEINS; APOLIPOPROTEINS C-I; FREE FATTY-ACIDS; APO A-I; SEVERE HYPERTRIGLYCERIDEMIA; CARDIOVASCULAR-DISEASE; MONOCLONAL-ANTIBODIES; CHYLOMICRON REMNANTS AB Background: Lipoprotein lipase (LPL) is a multifunctional protein and a key enzyme involved in the regulation of lipoprotein metabolism. We determined the lipoproteins to which LPL is bound in the pre-heparin and post heparin plasma. Methods: Tetrahydrolipstatin (THL), a potent inhibitor of serine lipases, was used to block the lipolytic activity of LPL, thereby preventing changes in the plasma lipoproteins due to ex vivo lipolysis. Gel filtration was performed to obtain the LPL elution profiles in plasma and the isolated remnant lipoproteins (RLP). Results: When ex vivo lipolytic activity was inhibited by THL in the post-heparin plasma, majority of the LPL was found in the VLDL elution range, specifically in the RLP as inactive dimers. However, in the absence of THL, most of the LPL was found in the HDL elution range as active dimers. Furthermore, majority of the LPL in the pre-heparin plasma was found in the RLP as inactive form, with broadly diffused lipoprotein profiles in the presence and absence of THL. Conclusions: It is suggested that during lipolysis in vivo, the endothelial bound LPL dimers generates RLP, forming circulating RLP-LPL complexes in an inactive form that subsequently binds and initiates receptor-mediated catabolism. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sato, Koichi; Okajima, Fumikazu] Gunma Univ, Inst Mol & Cellular Regulat, Maebashi, Gumma, Japan. [Miyashita, Kazuya] Immunobiol Labs, Fujioka, Gunma, Japan. [Imamura, Shigeyuki] Imamura Enzyme Technol Lab, Shizuoka, Japan. [Kobayashi, Junji] Kanazawa Med Univ, Dept Gen Med, Kanazawa, Ishikawa, Japan. [Stanhope, Kimber L.; Havel, Peter J.; Nakajima, Katsuyuki] Univ Calif Davis, Dept Nutr, Sch Vet Med, Dept Mol Biosci, Davis, CA 95616 USA. [Machida, Tetsuo; Sumino, Hiroyuki; Murakami, Masami; Nakajima, Katsuyuki] Gunma Univ, Grad Sch Med, Dept Clin Lab Med, 3-39-15 Showa Machi, Maebashi, Gumma, Japan. [Schaefer, Ernst; Nakajima, Katsuyuki] Tufts Univ, Sch Med, Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. RP Nakajima, K (reprint author), Gunma Univ, Grad Sch Med, Dept Clin Lab Med, 3-39-15 Showa Machi, Maebashi, Gumma, Japan. EM nakajimak05@ybb.ne.jp FU Ministry of Education, Culture, Sports, Sciences and Technology of Japan [26460640]; National Institutes of Health (NIH)/National Heart, Lung and Blood Institute; National Center for Research Resources, a component of the NIH; NIH Roadmap for Medical Research; Building Interdisciplinary Research Careers in Women's Health award - National Institute of Child Health and Human Development; Office of Research on Women's Health; Office of Dietary Supplements; National Institute of Aging FX This work was supported in part by a Grant-in-Aid (26460640) for General Scientific Research from the Ministry of Education, Culture, Sports, Sciences and Technology of Japan. The clinical trial (clinicaltrials.gov Identifier: NCT01103921) conducted at UC Davis was funded by National Institutes of Health (NIH)/National Heart, Lung and Blood Institute; National Center for Research Resources, a component of the NIH, and NIH Roadmap for Medical Research. Kimber Stanhope was supported by a Building Interdisciplinary Research Careers in Women's Health award funded by the National Institute of Child Health and Human Development, Office of Research on Women's Health, Office of Dietary Supplements, and the National Institute of Aging. The authors would like thank Dr. John Brunzell, the late professor of University of Washington, Seattle and Dr. Richard Havel, the late professor of University of California San Francisco, Cardiovascular Research Institute for their valuable discussions of concepts and leadership on remnant lipoprotein research during the last several decades. NR 56 TC 2 Z9 2 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0009-8981 EI 1873-3492 J9 CLIN CHIM ACTA JI Clin. Chim. Acta PD OCT 1 PY 2016 VL 461 BP 114 EP 125 DI 10.1016/j.cca.2016.06.020 PG 12 WC Medical Laboratory Technology SC Medical Laboratory Technology GA DW8ZM UT WOS:000383944000019 PM 27342999 ER PT J AU Larimore, EL Amundson, OL Bridges, GA McNeel, AK Cushman, RA Perry, GA AF Larimore, E. L. Amundson, O. L. Bridges, G. A. McNeel, A. K. Cushman, R. A. Perry, G. A. TI Changes in ovarian function associated with circulating concentrations of estradiol before a GnRH-induced ovulation in beef cows SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Estradiol; Steroidogenesis; Bovine; Follicle; Fixed-time ID GONADOTROPIN-RELEASING-HORMONE; FOLLICLE-STIMULATING-HORMONE; TIME ARTIFICIAL-INSEMINATION; RAT GRANULOSA-CELLS; LUTEINIZING-HORMONE; DAIRY-COWS; PREGNANCY ESTABLISHMENT; PREOVULATORY ESTRADIOL; EMBRYONIC-DEVELOPMENT; REPRODUCTIVE-TRACT AB These studies were conducted to evaluate causes for differences in circulating concentrations of estradiol before a GnRH-induced ovulation. Beef cows were synchronized by an injection of GnRH on day -7 and an injection of prostaglandin F2 alpha (PGF(2 alpha)) on day 0. In experiment 1, blood samples were collected every 3 h from PGF(2 alpha), on day 0 to hour 33 after PGF(2 alpha), and at slaughter (hour 36 to 42; n = 10). Cows were assigned to treatment group based on circulating concentrations of estradiol (E2): HighE2 vs LowE2. At slaughter, follicular fluid (FF) and granulosa cells were collected from the dominant follicle. In experiment 2, blood samples (n = 30) were collected every 8 h from PGF(2 alpha) until the dominant follicle was aspirated via ultrasound-guided follicular aspiration to collect FF and granulosa cells (hour 38 to 46). In experiment 1, HighE2 had increased abundance of 3 beta-hydroxysteroid dehydrogenase, cytochrome P450 aromatase, and LHR (P <= 0.02), and greater concentrations of estradiol and androstenedione (P <= 0.02) in the FF. In experiment 2, HighE2 had increased abundance of CYP11A1, 3 beta-hydroxysteroid dehydrogenase, cytochrome P450 aromatase, and LHR (P <= 0.03) vs either LowE2 or GnRHLowE2. There was a tendency (P = 0.07) for LH pulse frequency to be increased in both the GnRHLowE2 and HighE2 compared with LowE2. HighE2 cows experienced increas in circulating concentrations of estradiol compared with LowE2. In conclusion, animals with greater concentrations of circulating estradiol before fixed-time AI experienced an upregulation of the steroidogenic pathway during the preovulatory period. (C) 2016 Elsevier Inc. All rights reserved. C1 [Larimore, E. L.; Amundson, O. L.; Perry, G. A.] South Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. [Bridges, G. A.] Univ Minnesota, North Cent Res & Outreach Ctr, Grand Rapids, MI USA. [McNeel, A. K.; Cushman, R. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Bridges, G. A.] GAB Elanco Anim Hlth, Greenfield, IN USA. [McNeel, A. K.] Zoetis Anim Genet, Kalamazoo, MI USA. RP Perry, GA (reprint author), South Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. EM george.perry@sdstate.edu FU South Dakota Agricultural Experiment Station Hatch Funds; ARS project [5438-31000-093-00D] FX This research was funded in part by the South Dakota Agricultural Experiment Station Hatch Funds and by ARS project plan number 5438-31000-093-00D entitled "Strategies to Improve Heifer Selection and Heifer Development" (RAC). The authors gratefully acknowledge Zoetis for the donation of Factrel and Lutalyse, and Western Point Inc for the donation of EstroTect patches. NR 43 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD OCT PY 2016 VL 57 BP 71 EP 79 DI 10.1016/j.domaniend.2016.06.001 PG 9 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DX0GD UT WOS:000384038900009 PM 27565233 ER PT J AU Foote, AP Hales, KE Freetly, HC AF Foote, A. P. Hales, K. E. Freetly, H. C. TI Changes in acyl and total ghrelin concentrations and their association with dry matter intake, average daily gain, and feed efficiency of finishing beef steers and heifers SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Beef cattle; Feed efficiency; Feed intake regulation; Gut peptide ID GROWTH-HORMONE SECRETAGOGUE; PLASMA GHRELIN; CIRCULATING GHRELIN; LEPTIN CONCENTRATIONS; NUTRIENT RESTRICTION; CATTLE; RECEPTOR; PEPTIDE; ACTIVATION; STOMACH AB Ghrelin is a peptide hormone produced in the gut that is implicated in signaling appetite and regulating dry matter intake (DMI). The objective of this experiment was to determine the change in acyl ghrelin, total ghrelin, and the ghrelin ratio (acyl ghrelin/total ghrelin) over an 84-d DMI and average daily BW gain (ADG) measurement period and to determine the association of those ghrelin measurements with DMI, ADG, ADG:DMI ratio (G:F), and residual feed intake in finishing beef steers and heifers. Blood samples were collected on day 0 and day 83 before feeding and between 0730 h and 1130 h. Samples were analyzed for acyl and total ghrelin using commercially available RIA. DMI in steers was greater during the last 35-d period of the experiment compared with the first 35 d (P < 0.01) and was greater than heifers regardless of period (P < 0.01). Steers had greater acyl ghrelin concentrations on day 0 than heifers, but concentrations decreased by day 83 to equal concentrations in heifers (P < 0.01). Total ghrelin concentrations were lower on day 0 in heifers but increased by day 83 and did not differ from steers on day 83 (P < 0.01). A mixed model analysis was used to determine the association of ghrelin concentrations and ratio with production traits, independent of breed and sire effects. There was an interaction of day 0 acyl ghrelin concentrations with time of sample collection for 84-d DMI (P < 0.01), ADG (P < 0.01), and G:F (P = 0.09), indicating a general positive association of acyl ghrelin with production traits, but the association weakened as time of sample collection increased. The mean ghrelin ratio tended (P = 0.08) to be positively associated with DMI in the last 35-d period. The ghrelin ratio on day 0 interacted with time of sample collection for ADG and G:F (P < 0.05), indicating an overall positive association of the ghrelin ratio with ADG and G:F. Results indicate that ghrelin is associated with DMI, ADG, and feed efficiency of finishing beef cattle, and data lend more evidence that ghrelin is involved in appetite regulation of ad libitum fed cattle. Published by Elsevier Inc. C1 [Foote, A. P.; Hales, K. E.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Foote, AP (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Andrew.Foote@ars.usda.gov FU NIFA grant through the National Program for Genetic Improvement of Feed Efficiency in Beef Cattle [2011-68004-30214] FX This project was partially funded by NIFA grant 2011-68004-30214 through the National Program for Genetic Improvement of Feed Efficiency in Beef Cattle. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 23 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD OCT PY 2016 VL 57 BP 100 EP 107 DI 10.1016/j.domaniend.2016.05.004 PG 8 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DX0GD UT WOS:000384038900012 PM 27565236 ER PT J AU Sanchez, JJ Baerenklau, K Gonzalez-Caban, A AF Sanchez, Jose J. Baerenklau, Ken Gonzalez-Caban, Armando TI Valuing hypothetical wildfire impacts with a Kuhn-Tucker model of recreation demand SO FOREST POLICY AND ECONOMICS LA English DT Article DE Kuhn-Tucker demand system model; Forest recreation value; Hypothetical burn scenarios; Web-based survey; Nonmarket valuation ID BIKING DEMAND; FIRE; SYSTEMS; HIKING; VALUES AB This study uses a nonmarket valuation method to-investigate the recreation values of the San Jacinto Wilderness in southern California. The analysis utilizes survey data from a stated-choice experiment involving backcountiy visitors who responded to questions about hypothetical wildfire burn scenarios. Benefits of landscape preservation are derived using a Kuhn-Tucker (KT) demand system. Model results suggest that recreationists are attracted to sites with recent wildfires that can be viewed up-close. For example, recreational welfare estimates increased for sites that were partially affected by different types of wildfires, with the greatest gains being observed for the most recent wildfires. Per person mean seasonal willingness-to-pay varied from a low of $10 to a high of $48, for total gains ranging from $62,223 to $635,286. However, wildfires that cause trail closures create welfare losses. Seasonal losses per person for complete closure of particular sites range from $3 to $221, for total losses ranging from $29,600 to $2.9 million. Published by Elsevier B.V. C1 [Sanchez, Jose J.; Gonzalez-Caban, Armando] US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. [Baerenklau, Ken] Univ Calif Riverside, Riverside, CA 92521 USA. RP Sanchez, JJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM jsanchez@fs.fed.us FU Western Wildland Environmental Threat Assessment Center FX This work is based on the main author's PhD dissertation. An abbreviated version of this paper was presented at the XXIV IUFRO World Congress, Salt Lake City, Utah, 5-11 October 2014. Generous research funding was provided by the Western Wildland Environmental Threat Assessment Center through a 2011-2013 grant. We thank Andy Smith and the staff at San Bernardino National Forest San Jacinto Ranger District, and Bart Grant and staff at Mount San Jacinto State Park & Wilderness for their assistance in distributing recruitment flyers and providing forest usage data. We also thank UC Riverside students Noemi Villegas-Valadez and Yi-Chia Chu, and Hispanic Association of Colleges and Universities intern Jose Roman for field work assistance; and Rich Alldredge, John Loomis, Pete Schuhmann, and Javier Suarez for their useful comments provided in developing the survey design. We also want to thank Dan Phaneuf for providing the Matlab program to derive parameter and welfare estimates. Finally, we would like to thank two unanimous referees for their helpful comments in clarifying presentation of the material. NR 31 TC 1 Z9 1 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD OCT PY 2016 VL 71 BP 63 EP 70 DI 10.1016/j.forpol.2015.08.001 PG 8 WC Forestry SC Forestry GA DW7IZ UT WOS:000383825700008 ER PT J AU Walton, ZL Poudyal, NC Hepinstall-Cymerman, J Gaither, CJ Boley, BB AF Walton, Z. L. Poudyal, N. C. Hepinstall-Cymerman, J. Gaither, C. Johnson Boley, B. B. TI Exploring the role of forest resources in reducing community vulnerability to the heat effects of climate change SO FOREST POLICY AND ECONOMICS LA English DT Article DE Ecosystem services; Public health; Mortality; Adaptation; Counties; Land use; Heat index; Poisson Model ID WILDLAND FIRE RISK; SOCIAL VULNERABILITY; ECOSYSTEM SERVICES; UNITED-STATES; ISLAND; FLOOD AB While the growing literature on forest ecosystem services has examined the value and significance of a range of services, our understanding of the health-related benefits of ecosystem services from forests is still limited. To characterize the role of forest resources in reducing community vulnerability to the heat effects of climate change, a general index of heat vulnerability (HEVI) was developed through Principle Components Analysis (PCA) and subsequently used within ANVOA and Poisson regression to assess the relationship between the amount and type of forest resources (species, management regime, spatial pattern) and a county's vulnerability to the heat effects of climate change. Results of the ANOVA showed significant differences in the extent and characteristics of forests among counties experiencing different levels of heat vulnerability. The Poisson regression using county heat mortality as the dependent variable found forest characteristics to have a significant influence on heat mortality when other determinants of vulnerability were controlled. A negative and significant relationship was specifically found between forest area and heat related mortality, which supports the hypothesis that the extent of forest coverage helps to alleviate vulnerability associated with heat effects. These findings have important implications for understanding the role of forest ecosystem services in reducing a community's vulnerability to the heat effects of climate change. Findings will also be useful in guiding land use planning and preserving desirable forest characteristics to help communities adapt to climate change. (C) 2015 Elsevier B.V. All rights reserved. C1 [Hepinstall-Cymerman, J.; Boley, B. B.] Univ Georgia, Warne Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Poudyal, N. C.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Gaither, C. Johnson] US Forest Serv, USDA, Southern Res Stn, Athens, GA USA. [Walton, Z. L.] Dougherty & Dougherty Forest Serv Inc, Danielsville, GA USA. RP Poudyal, NC (reprint author), Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. EM npoudyal@utk.edu OI Poudyal, Neelam/0000-0003-1230-0679 FU USDA Forest Service, Southern Research Station [11-CA-11330144-049] FX Funding for this study was provided by the USDA Forest Service, Southern Research Station, Award # 11-CA-11330144-049. The authors are thankful to Dudley Hartell for his input and guidance during the initial designing of this study. NR 48 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD OCT PY 2016 VL 71 BP 94 EP 102 DI 10.1016/j.forpol.2015.09.001 PG 9 WC Forestry SC Forestry GA DW7IZ UT WOS:000383825700012 ER PT J AU Van Ekert, E Wang, M Miao, YG Brent, CS Hull, JJ AF Van Ekert, E. Wang, M. Miao, Y. -G. Brent, C. S. Hull, J. J. TI RNA interference-mediated knockdown of the Halloween gene Spookiest (CYP307B1) impedes adult eclosion in the western tarnished plant bug, Lygus hesperus SO INSECT MOLECULAR BIOLOGY LA English DT Article DE western tarnished plant bug; Lygus hesperus; Halloween gene; Spookiest; CYP307B1; RNA interference ID PLANTHOPPER LAODELPHAX-STRIATELLUS; INSECT BLATTELLA-GERMANICA; MISSISSIPPI RIVER DELTA; STRIPED STEM BORER; DROSOPHILA-MELANOGASTER; CYTOCHROME P450S; ECDYSTEROID BIOSYNTHESIS; SOGATELLA-FURCIFERA; NYMPHAL DEVELOPMENT; CHILO-SUPPRESSALIS AB Ecdysteroids play a critical role in coordinating insect growth, development and reproduction. A suite of cytochrome P450 monooxygenases coded by what are collectively termed Halloween genes mediate ecdysteroid biosynthesis. In this study, we describe cloning and RNA interference (RNAi)-mediated knockdown of the CYP307B1 Halloween gene (Spookiest) in the western tarnished plant bug, Lygus hesperus. Transcripts for Ly. hesperus Spookiest (LhSpot) were amplified from all life stages and correlated well with timing of the pre-moult ecdysteroid pulse. In adults, LhSpot was amplified from heads of both genders as well as female reproductive tissues. Heterologous expression of a LhSpot fluorescent chimera in cultured insect cells co-localized with a fluorescent marker of the endoplasmic reticulum/secretory pathway. RNAi-mediated knockdown of LhSpot in fifth instars reduced expression of ecdysone-responsive genes E74 and E75, and prevented adult development. This developmental defect was rescued following application of exogenous 20-hydroxyecdysone but not exogenous 7-dehydrocholesterol. The unequivocal RNAi effects on Ly. hesperus development and the phenotypic rescue by 20-hydroxyecdysone are causal proof of the involvement of LhSpot in ecdysteroid bio-synthesis and related developmental processes, and may provide an avenue for development of new control measures against Ly. hesperus. C1 [Van Ekert, E.; Brent, C. S.; Hull, J. J.] USDA ARS Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. [Wang, M.; Miao, Y. -G.] Zhejiang Univ, Hangzhou, Zhejiang, Peoples R China. RP Hull, JJ (reprint author), USDA ARS Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM joe.hull@ars.usda.gov FU China Scholarship Council; Cotton Inc. [12-373] FX The authors thank Daniel Langhorst for maintaining the Ly. hesperus colony and Lynn Forlow Jech for assistance with tissue dissections and insect cell culture maintenance. Funding was provided by the China Scholarship Council to M.W. and by Cotton Inc. (12-373) to C.S.B. and J.J.H. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 64 TC 0 Z9 0 U1 12 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD OCT PY 2016 VL 25 IS 5 BP 550 EP 565 DI 10.1111/imb.12242 PG 16 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DW0QC UT WOS:000383345000004 PM 27189651 ER PT J AU Shappell, NW AF Shappell, Nancy W. TI A Transformational Approach to Improve Interpretation of Environmental Data SO INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT LA English DT Letter C1 [Shappell, Nancy W.] ARS, Biosci Res Lab, USDA, Fargo, ND USA. RP Shappell, NW (reprint author), ARS, Biosci Res Lab, USDA, Fargo, ND USA. EM nancy.shappell@ars.usda.gov NR 1 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1551-3777 EI 1551-3793 J9 INTEGR ENVIRON ASSES JI Integr. Environ. Assess. Manag. PD OCT PY 2016 VL 12 IS 4 BP 613 EP 614 DI 10.1002/ieam.1810 PG 2 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DW0XH UT WOS:000383365800003 PM 27640408 ER PT J AU Kirker, GT Bishell, AB Zelinka, SL AF Kirker, Grant T. Bishell, Amy B. Zelinka, Samuel L. TI Electrical properties of wood colonized by Gloeophyllum trabeum SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Wood decay; Nondestructive evaluation; Electrical resistance measurements; Wood moisture content; Brown rot fungus; Ionic conduction ID MOISTURE-CONTENT; DECAY; TRANSLOCATION; FUNGI AB This paper examines how wood decay fungi affect the electrical resistance (resistivity) of wood to determine whether an electrical resistance probe could be deployed as a remote sensor in a wall to detect wood decay. Electrical resistance measurements were taken on wood blocks exposed to Gloeophyllum trabeum in a standard soil bottle tests (AWPA E10) as a function of time. To understand how and why resistance changed with time, results were compared against resistance measurements taken in sterilized, un-inoculated wood at high moisture contents; concentrations of certain mineral ions in the wood were also measured. Results indicate that most of the changes in electrical resistance in the soil bottle test could be described by changes in moisture content alone. When the moisture effects were removed, decay fungi decreased the resistance at small amounts of weight loss but increased the resistance at high weight loss. The results presented herein help to explain the mechanism by which empirical, electrical resistance nondestructive testing devices work and also the limitations on using resistance measurements to detect wood decay. Published by Elsevier Ltd. C1 [Kirker, Grant T.; Bishell, Amy B.; Zelinka, Samuel L.] US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Zelinka, SL (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM szelinka@fs.fed.us OI Zelinka, Samuel/0000-0002-1348-7730 FU Presidential Early Career Award for Scientists and Engineers (PECASE); Forest Products Laboratory of the USDA Forest Service FX SLZ acknowledges funding from the Presidential Early Career Award for Scientists and Engineers (PECASE). Research was funded by the Forest Products Laboratory of the USDA Forest Service. The authors acknowledge the contributions of Kolby Hirth, who performed the ICP-AES measurements. This paper was written and prepared by U.S. Government employees on official time and therefore is in the public domain and not subject to copyright in the United States. NR 30 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD OCT PY 2016 VL 114 BP 110 EP 115 DI 10.1016/j.ibiod.2016.06.004 PG 6 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA DW3FR UT WOS:000383528000015 ER PT J AU Bari, E Taghiyari, HR Naji, HR Schmidt, O Ohno, KM Clausen, CA Bakar, ES AF Bari, Ehsan Taghiyari, Hamid Reza Naji, Hamid Reza Schmidt, Olaf Ohno, Katie M. Clausen, Carol A. Bakar, Edi Suhaimi TI Assessing the destructive behaviors of two white-rot fungi on beech wood SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE Chemical composition; Mass loss; Mechanical properties; Destructive behavior; Pleurotus ostreatus; Trametes versicolor; White-rot ID TRAMETES-VERSICOLOR; PLEUROTUS-OSTREATUS; SELECTIVE DELIGNIFICATION; MECHANICAL-PROPERTIES; CHEMICAL-COMPOSITION; LIGNIN DEGRADATION; BROWN-ROT; STRENGTH; DECAY; LIGNOCELLULOSICS AB This research assessed the destructive behaviors of two white-rot fungi, Pleurotus ostreatus and Trametes versicolor and compared their degradation capabilities on solid oriental beech wood (Fagus orientalis Lipsky). Beech wood specimens were exposed to both fungi for a period of 120 days based on the specifications in the EN-113 standard. Mechanical properties and chemical composition of the specimens were measured every 15 days. Mass loss (ML) caused by the two fungi were not significantly different at the last stage of exposure, although T. versicolor showed higher ML at the middle exposure intervals. Compression strength parallel to grain, hardness, and impact bending values were higher in specimens exposed to P. ostreatus, although differences were not statistically significant. Microscopic images showed nearly the same decay patterns for both fungi. The results of the chemical assessment indicated that degradation of cell wall components was approximately the same for both fungi, but in some case T versicolor showed a slightly higher potential to decay compared to P. ostreatus, especially in the early stages of exposure. The two fungi differed in their ability to reduce degree of polymerization (DP) holocellulose of beech wood. It was concluded that the destructive behaviors ability of both fungi was considerable. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Bari, Ehsan] Tech Fac Sari 2, Dept Wood Sci & Engn, Sari, Iran. [Bari, Ehsan] Univ Agr & Nat Resources, Dept Wood & Paper Sci Nat Resources, Sari, Iran. [Taghiyari, Hamid Reza] SRTTU, Fac Civil Engn, Wood Sci & Technol Dept, Tehran, Iran. [Naji, Hamid Reza] Univ Ilam, Dept Forest Sci, Ilam, Iran. [Bakar, Edi Suhaimi] Univ Putra Malaysia, Inst Trop Forestry & Forest Prod, Serdang 43400, Selangor, Malaysia. [Schmidt, Olaf] Univ Hamburg, Dept Wood Biol, Leuschnerstr 91, D-21031 Hamburg, Germany. [Ohno, Katie M.; Clausen, Carol A.] USDA Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Bari, E (reprint author), Tech Fac Sari 2, Dept Wood Sci & Engn, Sari, Iran.; Taghiyari, HR (reprint author), SRTTU, Fac Civil Engn, Wood Sci & Technol Dept, Tehran, Iran. EM bari_lenzites@yahoo.com; htaghiyari@srttu.edu; hrnaji2000@gmail.corn; olaf.schmidt@uni-hamburg.de; kohno@fs.fed.us; cclausen@fs.fed.us OI Bari, Ehsan/0000-0002-9144-0663 NR 62 TC 1 Z9 1 U1 10 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD OCT PY 2016 VL 114 BP 129 EP 140 DI 10.1016/j.ibiod.2016.06.010 PG 12 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA DW3FR UT WOS:000383528000018 ER PT J AU Yelle, DJ Ralph, J AF Yelle, Daniel J. Ralph, John TI Characterizing phenol-formaldehyde adhesive cure chemistry within the wood cell wall SO INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES LA English DT Article DE Adhesives for wood; Phenolic; Wood and wood composites; Adhesion by chemical bonding; Arylglycerol-beta-aryl ether ID SOLUTION-STATE NMR; MODEL EXPERIMENTS; ALKALINE-DEGRADATION; TECHNICAL LIGNINS; ETHER STRUCTURES; KRAFT LIGNINS; DURCH ALKALI; WHITE LIQUOR; GLUE LINES; SPECTROSCOPY AB Adhesive bonding of wood using phenol-formaldehyde remains the industrial standard in wood product bond durability. Not only does this adhesive infiltrate the cell wall, it also is believed to form primary bonds with wood cell wall polymers, particularly guaiacyl lignin. However, the mechanism by which phenol-formaldehyde adhesive integrally interacts and bonds to lignin within the cell wall remains unclear. We used recently developed solubilization methodologies in conjunction with two-dimensional H-1-C-13 solution-state NMR spectroscopy of ball-milled pine earlywood and latewood bonded assemblies to characterize the chemical modification of wood cell wall polymers after phenol-formaldehyde curing at various cooking times. The results showed that the highly alkaline resin at 140 degrees C decreased the frequency of the principal arylglycerol-beta-aryl ether interunit linkage by eighty percent in earlywood and by twenty percent in latewood. The presence of newly formed diarylmethanes between guaiacyl lignin units and phenolic methylols was confirmed via NMR spectra of the aliphatic methylene and aromatic regions. The phenol-formaldehyde cure chemistry showed that o-p methylene bridges dominated in both earlywood and latewood cell walls, but the propensity of p-p substitution is higher in the latewood cell wall. Our results provide evidence for a simultaneous wood polymer degradation and guaiacyl unit C5 bond formation that occurs during phenol-formaldehyde curing. This competition may be necessary for developing good bond durability between the adhesive and wood. Published by Elsevier Ltd. C1 [Yelle, Daniel J.] USDA Forest Serv, US Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Ralph, John] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Dept Biochem, 1552 Univ Ave, Madison, WI 53726 USA. [Ralph, John] Univ Wisconsin, Wisconsin Energy Inst, 1552 Univ Ave, Madison, WI 53726 USA. RP Yelle, DJ (reprint author), USDA Forest Serv, US Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM dyelle@fs.fed.us FU DOE Great Lakes Bioenergy Research Center (DOE Office of Science) [BER DE-FC02-07ER64494] FX We thank Prof. Chip Frazier for very insightful discussions. JR was funded in part by the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). NR 65 TC 0 Z9 0 U1 28 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0143-7496 EI 1879-0127 J9 INT J ADHES ADHES JI Int. J. Adhes. Adhes. PD OCT PY 2016 VL 70 BP 26 EP 36 DI 10.1016/j.ijadhadh.2016.05.002 PG 11 WC Engineering, Chemical; Materials Science, Multidisciplinary SC Engineering; Materials Science GA DV9YF UT WOS:000383297400004 ER PT J AU Broderick, GA Colombini, S Costa, S Karsli, MA Faciola, AP AF Broderick, Glen A. Colombini, Stefania Costa, Sara Karsli, Mehmet A. Faciola, Antonio P. TI Chemical and ruminal in vitro evaluation of Canadian canola meals produced over 4 years SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE canola meal; chemical composition; ruminal degradation; rumen-undegraded protein ID LACTATING DAIRY-COWS; DIFFERENT PROTEIN SUPPLEMENTS; DRIED DISTILLERS GRAINS; SOYBEAN-MEAL; AMINO-ACIDS; HEAT-TREATMENT; DETERGENT FIBER; MILK-PRODUCTION; CRUDE PROTEIN; DRY-MATTER AB To test the effects of year and processing plant on the nutritional value of canola meal (CM), 3 CM samples/yr were collected from each of 12 Canadian production plants over 4 yr (total = 144). Samples of CM were analyzed for differences in chemical composition and for in vitro ruminal protein degradability using the Michaelis-Menten inhibitor in vitro (MMIIV) method. In the MMIIV method, protein degradation rate (k(d)) was estimated by 2 methods: from net release (i.e., blank corrected) of (1) ammonia plus AA determined by o-phthaldialdehyde fluorescence (OPA(F)) assay or (2) ammonia, AA, plus oligopeptides determined by o-phthaldialdehyde absorbance (OPA(A)) assay; rumen-undegradable protein (RUP) was computed assuming passage rates of 0.16 and 0.06/h for, respectively, soluble and insoluble protein. Casein, solvent soybean meal (SSBM), and expeller soybean meal (ESBM) were included in all incubations as standard proteins. Differences among years and plants were assessed using the mixed procedures of SAS. Small but significant differences were found in CM among years for chemical composition, including N solubility; some of these differences may have been related to changes in our analytical methods over time. However, adjustment of degradation activity of individual in vitro incubations based on the mean degradation activity over all incubations yielded k(d) and RUP that did not differ by year using either assay. Simultaneously incubating CM samples from 2 yr in the same in vitro runs confirmed that no year effects existed for k(d) or RUP. Differences existed in chemical composition of CM among the 12 processing plants over the 4 yr of sample collection. Moreover, consistent differences in k(d) and RUP were observed among plants: k(d) ranged from 0.069 to 0.113/h (OPA(A) assay) and 0.075 to 0.120/h (OPA(F) assay), and RUP estimates ranged from 51 to 43% (OPA(A) assay) and 49 to 41% (OPA(F) assay). Regression of k(d) on insoluble N content of CM yielded correlation coefficients (R-2) = 0.40 (OPA(A) assay) and 0.42 (OPA(F) assay), and regressions of k(d) on NDIN and N-fraction B-3 yielded R-2 < 0.02. Mean estimates from both OPAA and OPAF assays for casein, SSBM, ESBM, and CM were, respectively, k(d) = 0.764, 0.161, 0.050, and 0.093/h and RUP = 18, 33, 56, and 45%. A range of 8 percentage units from lowest to highest RUP suggests that substantial differences exist in metabolizable protein content of CM produced by different processing plants. C1 [Broderick, Glen A.] ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. [Colombini, Stefania; Costa, Sara] Univ Milan, Dipartimento Sci Anim, I-20133 Milan, Italy. [Karsli, Mehmet A.] Kirikkale Univ, Fac Vet, Dept Anim Nutr, TR-71451 Kirikkale, Turkey. [Faciola, Antonio P.] Univ Nevada, Dept Agr Nutr & Vet Sci, Reno, NV 89557 USA. [Broderick, Glen A.] Broderick Nutr & Res LLC, 221 Glen Hollow Rd, Madison, WI 53705 USA. RP Broderick, GA (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA.; Broderick, GA (reprint author), Broderick Nutr & Res LLC, 221 Glen Hollow Rd, Madison, WI 53705 USA. EM gbroderi@wisc.edu FU Canola Council of Canada FX The authors gratefully acknowledge the of the barn crew at the University of Wisconsin-Madison Dairy Cattle Center and the US Dairy Forage Research Center Farm (Prairie du Sac, WI) for care and feeding of the rumen donor cows; Wendy Radloff, Mary Becker, Eduardo Marostegan de Paula, and Ian Kraus for assisting with sampling and laboratory analyses; Peter Crump of the University of Wisconsin-Madison for assisting with statistical analyses; and the Canola Council of Canada for partial funding of this project. NR 45 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD OCT PY 2016 VL 99 IS 10 BP 7956 EP 7970 DI 10.3168/jds.2016-11000 PG 15 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DW0KB UT WOS:000383328200025 PM 27639102 ER PT J AU Kaniyamattam, K Elzo, MA Cole, JB De Vries, A AF Kaniyamattam, K. Elzo, M. A. Cole, J. B. De Vries, A. TI Stochastic dynamic simulation modeling including multitrait genetics to estimate genetic, technical, and financial consequences of dairy farm reproduction and selection strategies SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE multitrait genetics; modeling; selection; profit ID CATTLE BREEDING SCHEMES; GENOMIC SELECTION; SEXED SEMEN; ARTIFICIAL-INSEMINATION; PROGRAMS; HERD; LACTATION; TECHNOLOGIES; IMPROVEMENT; CONCEPTION AB The objective of this study was to develop a daily stochastic dynamic dairy simulation model that included multitrait genetics and to evaluate the effects of reduced genetic models and various reproduction and selection strategies on the genetic, technical, and financial performance of a dairy herd. The 12 correlated genetic traits included in the 2014 lifetime net merit (NM$) index were modeled for each animal. For each animal, a true breeding value (TBV) for each trait was calculated as the average of the sire's and dam's TBV, plus a fraction of the inbreeding and Mendelian sampling variability. Similarly, an environmental component for each trait was calculated and was partitioned into a permanent and a daily (temporary) effect. The combined TBV and environmental effects were converted into the phenotypic performance of each animal. Hence, genetics and phenotypic performances were associated. Estimated breeding values (EBV) were also simulated. Genetic trends for each trait for the service sire were based on expected trends in US Holsteins. Surplus heifers were culled based on various ranking criteria to maintain a herd size of 1,000 milking cows. In the first 8 scenarios, culling of surplus heifers was either random or based on the EBV of NM$. Four different genetic models, depending on the presence or absence of genetic trends or genetic and environmental correlations, or both, were evaluated to measure the effect of excluding multitrait genetics on animal performance. In the last 5 scenarios, the full genetic model was used and culling of surplus heifers was either random or based on the EBV of NM$ or the EBV of milk. Sexed semen use and reliability of the EBV were also varied. Each scenario was simulated for 15 yr into the future. Results showed that genetic models without all 12 genetic trends and genetic and environmental correlations provided biased estimates of the genetic, technical, and financial performance of the dairy herd. Average TBV of NM$ of all cows in the herd was $263 greater in yr 15 in a scenario that combined sexed semen use in heifers and culling of surplus heifers with the lowest EBV of NM$, compared with a scenario that used only conventional semen and surplus heifers were culled randomly. The average TBV of daughter pregnancy rate of all cows in the herd was 1.25 percentage points greater in yr 15 in a scenario that combined using sexed semen in heifers as well as culling of surplus heifers ranked by EBV of NM$, compared with a scenario using conventional semen only as well as culling surplus heifers ranked by EBV of milk. In conclusion, the multitrait genetics model resulted in improved estimates of genetic, technical, and financial effects and appears useful to evaluate consequences of various reproduction and selection strategies within a dairy farm. C1 [Kaniyamattam, K.; Elzo, M. A.; De Vries, A.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Cole, J. B.] USDA ARS, Anim Improvement Program, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP De Vries, A (reprint author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. EM devries@ufl.edu OI Cole, John/0000-0003-1242-4401 FU USDA-National Institute of Food and Agriculture (NIFA; Washington, DC) Agriculture and Food Research Initiative (AFRI) titled "Improving Fertility of Dairy Cattle Using Translational Genomics" [2013-68004-20365]; "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the Agricultural Research Service of the USDA [1265-31000-096-00] FX This study was financially supported by USDA-National Institute of Food and Agriculture (NIFA; Washington, DC) Agriculture and Food Research Initiative (AFRI) grant award 2013-68004-20365 titled "Improving Fertility of Dairy Cattle Using Translational Genomics." J. B. Cole was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the Agricultural Research Service of the USDA. NR 42 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD OCT PY 2016 VL 99 IS 10 BP 8187 EP 8202 DI 10.3168/jds.2016-11136 PG 16 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DW0KB UT WOS:000383328200045 PM 27497901 ER PT J AU Register, KB Nicholson, TL Brunelle, BW AF Register, Karen B. Nicholson, Tracy L. Brunelle, Brian W. TI Comparison of ribotyping and sequence-based typing for discriminating among isolates of Bordetella bronchiseptica SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Bordetella bronchiseptica; MLST; Ribotyping; Pertactin; pubMLST ID CYSTIC-FIBROSIS; PERTACTIN; INFECTION; PERTUSSIS; IDENTIFICATION; PARAPERTUSSIS; TRANSMISSION; PNEUMONIA; VACCINES; PATIENT AB Pvull ribotyping and MLST are each highly discriminatory methods for genotyping Bordetella bronchiseptica, but a direct comparison between these approaches has not been undertaken. The goal of this study was to directly compare the discriminatory power of Pvull ribotyping and MLST, using a single set of geographically and genetically diverse strains, and to determine whether subtyping based on repeat region sequences of the pertactin gene (pm) provides additional resolution. One hundred twenty-two isolates were analyzed, representing 11 mammalian or avian hosts, sourced from the United States, Europe, Israel and Australia. Thirty-two ribotype patterns were identified; one isolate could not be typed. In comparison, all isolates were typeable by MLST and a total of 30 sequence types was identified. An analysis based on Simpson's Index of Diversity (SID) revealed that ribotyping and MLST are nearly equally discriminatory, with SIDs of 0.920 for ribotyping and 0.919 for MIST. Nonetheless, for ten ribotypes and eight MLST sequence types, the alternative method discriminates among isolates that otherwise type identically. Pairing pm repeat region typing with ribotyping yielded 54 genotypes and increased the SID to 0.954. Repeat region typing combined with MIST resulted in 47 genotypes and an SID of 0.944. Given the technical and practical advantages of MLST over ribotyping, and the nominal difference in their SIDs, we conclude MLST is the preferred primary typing tool. We recommend the combination of MIST and pm repeat region typing as a high-resolution, objective and standardized approach valuable for investigating the population structure and epidemiology of B. bronchiseptica. Published by Elsevier B.V. C1 [Register, Karen B.] ARS, USDA, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. [Nicholson, Tracy L.] ARS, USDA, Virus & Prion Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. [Brunelle, Brian W.] ARS, USDA, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. RP Register, KB (reprint author), ARS, USDA, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM karen.register@ars.usda.gov FU Wellcome Trust FX The authors gratefully acknowledge the excellent technical assistance of William Boatwright, Michael Mullins and Sarah Shore and thank Lea Ann Hobbs and David Alt at the NADC Genomics Unit for DNA sequence data. We are indebted to Geoff Foster, Ronald Welsh and Andy Tomlinson for sharing isolates from their private collections. This publication made use of the Bordetella Multilocus Sequence Typing website (http://pubmlst.org/bordetella/) developed by Keith Jolley and sited at the University of Oxford (Jolley and Maiden, 2010, BMC Bioinformatics, 11:595). The development of this site has been funded by the Wellcome Trust. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 36 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD OCT PY 2016 VL 129 BP 117 EP 126 DI 10.1016/j.mimet.2016.08.008 PG 10 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DW8YN UT WOS:000383941500020 PM 27542997 ER PT J AU Ramirez-Carvajal, L Diaz-San Segundo, F Ramirez-Medina, E Rodriguez, LL de los Santos, T AF Ramirez-Carvajal, Lisbeth Diaz-San Segundo, Fayna Ramirez-Medina, Elizabeth Rodriguez, Luis L. de los Santos, Teresa TI Constitutively Active IRF7/IRF3 Fusion Protein Completely Protects Swine against Foot-and-Mouth Disease SO JOURNAL OF VIROLOGY LA English DT Article ID I INTERFERON; VIRUS; VACCINES; COMBINATION; ALPHA; DIFFERENTIATION; INFECTIVITY; VALIDATION; EXPRESSION; CHALLENGE AB Foot-and-mouth disease (FMD) remains one of the most devastating livestock diseases around the world. Several serotype-specific vaccine formulations exist, but they require about 5 to 7 days to induce protective immunity. Our previous studies have shown that a constitutively active fusion protein of porcine interferon (IFN) regulatory factors (IRF) 7 and 3 [IRF7/3(5D)] strongly induced type I IFN and antiviral genes in vitro and prevented mortality in an FMD mouse model when delivered with a replication-defective adenoviral vector [Ad5-poIRF7/3(5D)]. Here, we demonstrate that pigs treated with 10(8), 10(9), or 10(10) PFU of Ad5-poIRF7/3(5D) 24 h before FMDV challenge were fully protected from FMD clinical signs and did not develop viremia, virus shedding or antibodies against FMDV nonstructural proteins. Pigs treated with Ad5-poIRF7/3(5D) had higher levels of IFN and antiviral activity in serum, and upregulated expression of several IFN-stimulated genes in peripheral blood mononuclear cells, compared to pigs treated with Ad5-Blue vector control. Importantly, treatment of porcine cultured cells with Ad5-poIRF7/3(5D) inhibited the replication of all 7 FMDV serotypes. In vitro experiments using cultured embryonic fibroblasts derived from IFN receptor knockout mice suggested that the antiviral response induced by Ad5-poIRF7/3(5D) was dependent on type I and III IFN pathways; however, experiments with mice demonstrated that a functional type I IFN pathway mediates Ad5-poIRF7/3(5D) protection conferred in vivo. Our studies demonstrate that inoculation with Ad5-poIRF7/3(5D) completely protects swine against FMD by inducing a strong type I IFN response and highlights its potential application to rapidly and effectively prevent FMDV replication and dissemination. IMPORTANCE Foot-and-mouth disease virus (FMDV) causes a fast-spreading disease that affects farm animals, with economically and socially devastating consequences. Our study shows that inoculation with a constitutively active transcription factor, namely, a fusion protein of porcine interferon (IFN) regulatory factors (IRF) 7 and 3 delivered by an adenovirus vector [Ad5-poIRF7/3(5D)], is a new effective treatment to prevent FMD in swine. Animals pretreated with Ad5-poIRF7/3(5D) 1 day before being exposed to FMDV were completely protected from viral replication and clinical disease. It is noteworthy that the doses of Ad5-poIRF7/3(5D) required for protection are lower than those previously reported for similar approaches using Ad5 vectors delivering type I, II, or III IFN, suggesting that this novel strategy would be economically appealing to counteract FMD. Our results also indicate that a dynamic interplay among different components of pigs' innate immune defenses allows potent antiviral effects after Ad5-poIF7/3(5D) administration. C1 [Ramirez-Carvajal, Lisbeth; Diaz-San Segundo, Fayna; Ramirez-Medina, Elizabeth; Rodriguez, Luis L.; de los Santos, Teresa] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Ramirez-Carvajal, Lisbeth; Ramirez-Medina, Elizabeth] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. [Diaz-San Segundo, Fayna] Univ Connecticut, CANR, Dept Pathobiol & Vet Sci, Storrs, CT USA. RP de los Santos, T (reprint author), ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM teresa.delossantos@ars.usda.gov FU U.S. Department of Agriculture (USDA) [Cris 1940-32000-057-00D]; U.S. Department of Homeland Security (DHS) [HSHQDC-11-X-00189, HSHQPM-13-X-00113] FX This work, including the efforts of Teresa de los Santos, Lisbeth Ramirez-Carvajal, Fayna Diaz San Segundo, Elizabeth Ramirez-Medina, and Luis Rodriguez, was funded by U.S. Department of Agriculture (USDA) (Cris 1940-32000-057-00D). This work, including the efforts of Fayna Diaz San Segundo and Elizabeth Ramirez-Medina, was funded by U.S. Department of Homeland Security (DHS) (HSHQDC-11-X-00189 and HSHQPM-13-X-00113). NR 51 TC 0 Z9 0 U1 10 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD OCT PY 2016 VL 90 IS 19 BP 8809 EP 8821 DI 10.1128/JVI.00800-16 PG 13 WC Virology SC Virology GA DW6LH UT WOS:000383761900036 PM 27466421 ER PT J AU Klosterman, SJ Rollins, JR Sudarshana, MR Vinatzer, BA AF Klosterman, S. J. Rollins, J. R. Sudarshana, M. R. Vinatzer, B. A. TI Disease Management in the Genomics Era-Summaries of Focus Issue Papers SO PHYTOPATHOLOGY LA English DT Editorial Material C1 [Klosterman, S. J.] USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. [Rollins, J. R.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Sudarshana, M. R.] USDA ARS, One Shields Ave, Davis, CA 95616 USA. [Vinatzer, B. A.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. RP Klosterman, SJ (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM steve.klosterman@ars.usda.gov NR 17 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1068 EP 1070 DI 10.1094/PHYTO-07-16-0276-FI PG 3 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100001 ER PT J AU Poudel, R Jumpponen, A Schlatter, DC Paulitz, TC Gardener, BBM Kinkel, LL Garrett, KA AF Poudel, R. Jumpponen, A. Schlatter, D. C. Paulitz, T. C. Gardener, B. B. McSpadden Kinkel, L. L. Garrett, K. A. TI Microbiome Networks: A Systems Framework for Identifying Candidate Microbial Assemblages for Disease Management SO PHYTOPATHOLOGY LA English DT Article DE biocontrol; networks; phytobiome; Quercus macrocarpa; Triticum aestivum ID QUERCUS-MACROCARPA PHYLLOSPHERE; RHIZOSPHERE MICROBIOME; BACTERIAL COMMUNITIES; NATURAL SUPPRESSION; ECOLOGICAL NETWORKS; FUNGAL COMMUNITIES; CROPPING SYSTEMS; ROOT MICROBIOME; PLANT HEALTH; BARE PATCH AB Network models of soil and plant microbiomes provide new opportunities for enhancing disease management, but also challenges for interpretation. We present a framework for interpreting microbiome networks, illustrating how observed network structures can be used to generate testable hypotheses about candidate microbes affecting plant health. The framework includes four types of network analyses. "General network analysis" identifies candidate taxa for maintaining an existing microbial community. "Host-focused analysis" includes a node representing a plant response such as yield, identifying taxa with direct or indirect associations with that node. "Pathogen focused analysis" identifies taxa with direct or indirect associations with taxa known a priori as pathogens. "Disease-focused analysis" identifies taxa associated with disease. Positive direct or indirect associations with desirable outcomes, or negative associations with undesirable outcomes, indicate candidate taxa. Network analysis provides characterization not only of taxa with direct associations with important outcomes such as disease suppression, biofertilization, or expression of plant host resistance, but also taxa with indirect associations via their association with other key taxa. We illustrate the interpretation of network structure with analyses of microbiomes in the oak phyllosphere, and in wheat rhizosphere and bulk soil associated with the presence or absence of infection by Rhizoctonia solani. C1 [Poudel, R.; Garrett, K. A.] Univ Florida, Dept Plant Pathol, Inst Sustainable Food Syst, Gainesville, FL 32611 USA. [Poudel, R.; Garrett, K. A.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Jumpponen, A.] Kansas State Univ, Div Biol, Ackert Hall, Manhattan, KS 66506 USA. [Jumpponen, A.] Kansas State Univ, Ecol Genom Inst, Manhattan, KS 66506 USA. [Schlatter, D. C.; Paulitz, T. C.] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. [Gardener, B. B. McSpadden] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Kinkel, L. L.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Gardener, B. B. McSpadden] Sustane Nat Fertilizers, Cannon Falls, MN 55009 USA. RP Poudel, R; Garrett, KA (reprint author), Univ Florida, Dept Plant Pathol, Inst Sustainable Food Syst, Gainesville, FL 32611 USA.; Poudel, R; Garrett, KA (reprint author), Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. EM rp3448@ufl.edu; karengarrett@ufl.edu OI Poudel, Ravin/0000-0003-2622-3889 FU Ceres Trust; USDA NCR SARE Research and Education [LNC13-355]; CGIAR Research Program for Roots, Tubers and Bananas; USDA NIFA Grant [2015-51181-24257]; US NSF Grant [DBI-1300426]; University of Tennessee, Knoxville; US NSF as part of the joint NSF-NIH Ecology of Infectious Disease program [EF-0525712]; US NSF [DEB-0516046]; University of Florida FX KAG and RP developed the framework with input from all authors; AJ and TCP provided case study data; RP and DCS analyzed the case study data; RR KAG, AJ, DCS, TCP, BMG, and LLK wrote the manuscript. We appreciate the helpful reviews provided by Phytopathology reviewers. We appreciate support for this work from The Ceres Trust, USDA NCR SARE Research and Education Grant LNC13-355, CGIAR Research Program for Roots, Tubers and Bananas, USDA NIFA Grant 2015-51181-24257, US NSF Grant DBI-1300426 to NIMBioS with additional support from The University of Tennessee, Knoxville (Current Issues in Statistical Ecology Workshop), US NSF Grant EF-0525712 as part of the joint NSF-NIH Ecology of Infectious Disease program, US NSF Grant DEB-0516046, and the University of Florida. NR 85 TC 2 Z9 2 U1 52 U2 52 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1083 EP 1096 DI 10.1094/PHYTO-02-16-0058-FI PG 14 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100003 ER PT J AU Timilsina, S Abrahamian, P Potnis, N Minsavage, GV White, FF Staskawicz, BJ Jones, JB Vallad, GE Goss, EM AF Timilsina, Sujan Abrahamian, Peter Potnis, Neha Minsavage, Gerald V. White, Frank F. Staskawicz, Brian J. Jones, Jeffrey B. Vallad, Gary E. Goss, Erica M. TI Analysis of Sequenced Genomes of Xanthomonas perforans Identifies Candidate Targets for Resistance Breeding in Tomato SO PHYTOPATHOLOGY LA English DT Article DE type III effectors; avirulence; recombination ID CAMPESTRIS PV. VESICATORIA; BACTERIAL SPOT DISEASE; REVEALS DIVERSITY; EFFECTOR AVRBST; FIELD STRAINS; CELL-DEATH; RACE T3; PEPPER; VIRULENCE; PENNELLII AB Bacterial disease management is a challenge for modern agriculture due to rapid changes in pathogen populations. Genome sequences for hosts and pathogens provide detailed information that facilitates effector based breeding strategies. Tomato genotypes have gene-for-gene resistance to the bacterial spot pathogen Xanthomonas perforans. The bacterial spot populations in Florida shifted from tomato race 3 to 4, such that the corresponding tomato resistance gene no longer recognizes the effector protein AvrXv3. Genome sequencing showed variation in effector profiles among race 4 strains collected in 2006 and 2012 and compared with a race 3 strain collected in 1991. We examined variation in putative targets of resistance among Florida strains of X. perforans collected from 1991 to 2006. Consistent with race change, avrXv3 was present in race 3 strains but nonfunctional in race 4 strains due to multiple independent mutations. Effectors xopJ4 and avrBs2 were unchanged in all strains. The effector avrBsT was absent in race 3 strains collected in the 1990s but present in race 3 strains collected in 2006 and nearly all race 4 strains. These changes in effector profiles suggest that xopJ4 and avrBsT are currently the best targets for resistance breeding against bacterial spot in tomato. C1 [Timilsina, Sujan; Minsavage, Gerald V.; White, Frank F.; Jones, Jeffrey B.; Goss, Erica M.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Timilsina, Sujan; Abrahamian, Peter; Vallad, Gary E.] Univ Florida, Gulf Coast Res & Educ Ctr, Wimauma, FL USA. [Potnis, Neha] USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC USA. [Staskawicz, Brian J.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Goss, Erica M.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA. RP Goss, EM (reprint author), Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA.; Goss, EM (reprint author), Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA. EM emgoss@ufl.edu FU United States Department of Agriculture National Institute of Food and Agriculture Specialty Crop Research Initiative program; Florida Department of Agriculture and Consumer Services Specialty Crops block grant program FX We thank B. Vinatzer and three anonymous reviewers for their comments on the manuscript. This research was supported in part with funds from the United States Department of Agriculture National Institute of Food and Agriculture Specialty Crop Research Initiative program and the Florida Department of Agriculture and Consumer Services Specialty Crops block grant program. NR 31 TC 1 Z9 1 U1 12 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1097 EP 1104 DI 10.1094/PHYTO-03-16-0119-FI PG 8 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100004 ER PT J AU Withers, S Gongora-Castillo, E Gent, D Thomas, A Ojiambo, PS Quesada-Ocampo, LM AF Withers, S. Gongora-Castillo, E. Gent, D. Thomas, A. Ojiambo, P. S. Quesada-Ocampo, L. M. TI Using Next-Generation Sequencing to Develop Molecular Diagnostics for Pseudoperonospora cubensis, the Cucurbit Downy Mildew Pathogen SO PHYTOPATHOLOGY LA English DT Article ID REAL-TIME PCR; LINEAGE-SPECIFIC GENES; PLASMOPARA-HALSTEDII; SWEET BASIL; 1ST REPORT; P-HUMULI; GENOME; DNA; PLANT; TOOL AB Advances in next-generation sequencing (NGS) allow for rapid development of genomics resources needed to generate molecular diagnostics assays for infectious agents. NGS approaches are particularly helpful for organisms that cannot be cultured, such as the downy mildew pathogens, a group of biotrophic obligate oomycetes that infect crops of economic importance. Unlike most downy mildew pathogens that are highly host-specific, Pseudoperonospora cubensis causes disease on a broad range of crops belonging to the family Cucurbitaceae. In this study, we identified candidate diagnostic markers for P. cubensis by comparing NGS data from a diverse panel of P. cubensis and P. humuli isolates, two very closely related oomycete species. P cubensis isolates from diverse hosts and geographical regions in the United States were selected for sequencing to ensure that candidates were conserved in P cubensis isolates infecting different cucurbit hosts. Genomic regions unique to and conserved in P cubensis isolates were identified through bioinformatics. These candidate regions were then validated using PCR against a larger collection of isolates from P cubensis, P. humuli, and other oomycetes. Overall seven diagnostic markers were found to be specific to P. cubensis. These markers could be used for pathogen diagnostics on infected tissue, or adapted for monitoring airborne inoculum with real-time PCR and spore traps. C1 [Withers, S.; Gongora-Castillo, E.; Thomas, A.; Ojiambo, P. S.; Quesada-Ocampo, L. M.] North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Gent, D.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D.] Oregon State Univ, Corvallis, OR 97331 USA. [Thomas, A.; Ojiambo, P. S.] North Carolina State Univ, Ctr Integrated Fungal Res, Raleigh, NC 27695 USA. RP Quesada-Ocampo, LM (reprint author), North Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. EM lmquesad@ncsu.edu RI Gongora, Elsa/R-8854-2016; OI Gongora, Elsa/0000-0001-6327-6993; Quesada-Ocampo, Lina/0000-0002-9072-7531 FU U.S. Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS) Awards [13-8130-0254-CA, 13-8130-0274-CA]; USDA North Carolina Department of Agriculture (NCDA) Specialty Crop Block Grant Program (SCBGP) Award [12-25-B-16-88]; USDA-Agricultural Research Service (ARS) [NC02418, CRIS 2072-21000-046-00] FX We thank all the members of the Quesada lab for their valuable help, especially E. Wallace, M. Adams, C. Parada, and A. Rahman for technical assistance. We would like to acknowledge the technical assistance of N. Adair. This work was supported by the U.S. Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS) Awards 13-8130-0254-CA and 13-8130-0274-CA, the USDA North Carolina Department of Agriculture (NCDA) Specialty Crop Block Grant Program (SCBGP) Award 12-25-B-16-88, and USDA-Agricultural Research Service (ARS) under project numbers NC02418 and CRIS 2072-21000-046-00. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA or the ARS of any product or service to the exclusion of others that may be suitable. NR 61 TC 1 Z9 1 U1 18 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1105 EP 1116 DI 10.1094/PHYTO-10-15-0260-FI PG 12 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100005 ER PT J AU Chang, HX Lipka, AE Domier, LL Hartman, GL AF Chang, Hao-Xun Lipka, Alexander E. Domier, Leslie L. Hartman, Glen L. TI Characterization of Disease Resistance Loci in the USDA Soybean Germplasm Collection Using Genome-Wide Association Studies SO PHYTOPATHOLOGY LA English DT Article ID SUDDEN-DEATH SYNDROME; CYST-NEMATODE RESISTANCE; STEM ROT RESISTANCE; RUST PHAKOPSORA-PACHYRHIZI; MOSAIC-VIRUS; PHYTOPHTHORA-SOJAE; GENETIC-ANALYSIS; DIAPORTHE-PHASEOLORUM; UNDERLYING RESISTANCE; CONFERS RESISTANCE AB Genetic resistance is a key strategy for disease management in soybean. Over the last 50 years, soybean germplasm has been phenotyped for resistance to many pathogens, resulting in the development of disease resistant elite breeding lines and commercial cultivars. While biparental linkage mapping has been used to identify disease resistance loci, genome-wide association studies (GWAS) using high-density and high quality markers such as single nucleotide polymorphisms (SNPs) has become a powerful tool to associate molecular markers and phenotypes. The objective of our study was to provide a comprehensive understanding of disease resistance in the United States Department of Agriculture Agricultural Research Service Soybean Germplasm Collection by using phenotypic data in the public Germplasm Resources Information Network and public SNP data (SoySNP50K). We identified SNPs significantly associated with disease ratings from one bacterial disease, five fungal diseases, two diseases caused by nematodes, and three viral diseases. We show that leucine-rich repeat (LRR) receptor-like kinases and nucleotide binding site-LRR candidate resistance genes were enriched within the linkage disequilibrium regions of the significant SNPs. We review and present a global view of soybean resistance loci against multiple diseases and discuss the power and the challenges of using GWAS to discover disease resistance in soybean. C1 [Chang, Hao-Xun; Lipka, Alexander E.; Domier, Leslie L.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Domier, Leslie L.; Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA.; Hartman, GL (reprint author), USDA ARS, Urbana, IL 61801 USA. EM glen.hartman@ars.usda.gov OI Chang, Hao-Xun/0000-0002-4667-7741 FU USDA ARS FX We thank the curators of the USDA-ARS GRIN (ars-grin.gov) and SoyBase and the Soybean Breeder's Toolbox (soybase.org) along with funding support from USDA ARS. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by USDA-ARS or the University of Illinois and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 101 TC 2 Z9 2 U1 21 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1139 EP 1151 DI 10.1094/PHYTO-01-16-0042-FI PG 13 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100008 ER PT J AU Cadle-Davidson, L Gadoury, D Fresnedo-Ramirez, J Yang, SS Barba, P Sun, Q Demmings, EM Seem, R Schaub, M Nowogrodzki, A Kasinathan, H Ledbetter, C Reisch, BI AF Cadle-Davidson, Lance Gadoury, David Fresnedo-Ramirez, Jonathan Yang, Shanshan Barba, Paola Sun, Qi Demmings, Elizabeth M. Seem, Robert Schaub, Michelle Nowogrodzki, Anna Kasinathan, Hema Ledbetter, Craig Reisch, Bruce I. TI Lessons from a Phenotyping Center Revealed by the Genome-Guided Mapping of Powdery Mildew Resistance Loci SO PHYTOPATHOLOGY LA English DT Article ID MARKER-ASSISTED SELECTION; ERYSIPHE-NECATOR; VITIS SPP.; ENVIRONMENTAL COVARIABLES; ONTOGENIC RESISTANCE; HYPHAL GROWTH; GRAPEVINE; QTL; DEPLOYMENT; CROSSES AB The genomics era brought unprecedented opportunities for genetic analysis of host resistance, but it came with the challenge that accurate and reproducible phenotypes are needed so that genomic results appropriately reflect biology. Phenotyping host resistance by natural infection in the field can produce variable results due to the uncontrolled environment, uneven distribution and genetics of the pathogen, and developmentally regulated resistance among other factors. To address these challenges, we developed highly controlled, standardized methodologies for phenotyping powdery mildew resistance in the context of a phenotyping center, receiving samples of up to 140 grapevine progeny per F-1 family. We applied these methodologies to F1 families segregating for REN1- or REN2-mediated resistance and validated that some but not all bioassays identified the REN1 or REN2 locus. A point-intercept method (hyphal transects) to quantify colony density objectively at 8 or 9 days postinoculation proved to be the phenotypic response most reproducibly predicted by these resistance loci. Quantitative trait locus (QTL) mapping with genotyping-by-sequencing maps defined the REN1 and REN2 loci at relatively high resolution. In the reference PN40024 genome under each QTL, nucleotide-binding site-leucine-rich repeat candidate resistance genes were identified-one gene for REN1 and two genes for REN2. The methods described here for centralized resistance phenotyping and high resolution genetic mapping can inform strategies for breeding resistance to powdery mildews and other pathogens on diverse, highly heterozygous hosts. C1 [Cadle-Davidson, Lance; Schaub, Michelle] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. [Gadoury, David; Yang, Shanshan; Barba, Paola; Demmings, Elizabeth M.; Seem, Robert; Nowogrodzki, Anna; Kasinathan, Hema; Reisch, Bruce I.] Cornell Univ, Sch Integrat Plant Sci, New York State Agr Expt Stn, Geneva, NY 14456 USA. [Fresnedo-Ramirez, Jonathan; Sun, Qi] Cornell Univ, Bioinformat Facil, Ithaca, NY 14853 USA. [Ledbetter, Craig] USDA ARS, Crop Dis Pests & Genet Unit, Parlier, CA 93648 USA. [Barba, Paola] Inst Invest Agr INIA, La Platina Res Stn, Ave Santa Rosa 11610, Santiago 8831314, Chile. RP Cadle-Davidson, L (reprint author), USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. EM Lance.CadleDavidson@ars.usda.gov OI Fresnedo Ramirez, Jonathan/0000-0003-1985-2513 FU U.S. Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative [2011-51181-30635]; National Grape and Wine Initiative FX We thank P. Appleton, J. Lillis, B. Weldon, M. J. Welser, and numerous summer scholars, students, and visiting scientists for phenotyping support; K. Hyma for previously published contributions to the NY09.0805 parental maps; N. Ambriz and J. Andresen for 11-3527 vine maintenance and sampling; and S. Luce and A. Green for NY09.0805 vine maintenance and sampling. The U.S. Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative provided funding for this project (award 2011-51181-30635). The National Grape and Wine Initiative provided funding for S. Yang. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 43 TC 2 Z9 2 U1 14 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1159 EP 1169 DI 10.1094/PHYTO-02-16-0080-FI PG 11 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100010 ER PT J AU Mahoney, AK Babiker, EM Paulitz, TC See, D Okubara, PA Hulbert, SH AF Mahoney, A. K. Babiker, E. M. Paulitz, T. C. See, D. Okubara, P. A. Hulbert, S. H. TI Characterizing and Mapping Resistance in Synthetic-Derived Wheat to Rhizoctonia Root Rot in a Green Bridge Environment SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; HEXAPLOID WHEAT; PACIFIC-NORTHWEST; SOLANI AG-8; TAKE-ALL; REDUCED TILLAGE; PYTHIUM SPP.; R. ORYZAE; BARLEY; LINES AB Root rot caused by Rhizoctonia spp. is an economically important soil borne disease of spring-planted wheat in growing regions of the Pacific Northwest (PNW). The main method of controlling the disease currently is through tillage, which deters fanners from adopting the benefits of minimal tillage. Genetic resistance to this disease would provide an economic and environmentally sustainable resource for farmers. In this study, a collection of synthetic-derived genotypes was screened in high-inoculum and low-inoculum field environments. Six genotypes were found to have varying levels of resistance and tolerance to Rhizoctonia root rot. One of the lines, SPBC-3104 ('Vorobey'), exhibited good tolerance in the field and was crossed to susceptible PNW-adapted 'Louise' to examine the inheritance of the trait. A population of 190 BC1-derived recombinant inbred lines was assessed in two field green bridge environments and in soils artificially infested with Rhizoctonia solani AG8. Genotyping by sequencing and composite interval mapping identified three quantitative trait loci (QTL) controlling tolerance. Beneficial alleles of all three QTL were contributed by the synthetic-derived genotype SPCB-3104. C1 [Mahoney, A. K.; Babiker, E. M.; Paulitz, T. C.; See, D.; Okubara, P. A.; Hulbert, S. H.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Paulitz, T. C.; See, D.; Okubara, P. A.] USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. RP Hulbert, SH (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM scot_hulbert@wsu.edu OI Paulitz, Timothy/0000-0002-8885-3803 FU Washington State University [WNP00663] FX We thank the Washington Grains Commission for their continued support. PPNS number 0717, Department of Plant Pathology, College of Agriculture, Human and Natural Resource Sciences, Agricultural Research Center, Hatch Project Number WNP00663, Washington State University, Pullman 99164-6430. NR 49 TC 1 Z9 1 U1 9 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1170 EP 1176 DI 10.1094/PHYTO-02-16-0055-FI PG 7 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100011 ER PT J AU Gao, YY Liu, ZH Faris, JD Richards, J Brueggeman, RS Li, XH Oliver, RP McDonald, BA Friesen, TL AF Gao, Yuanyuan Liu, Zhaohui Faris, Justin D. Richards, Jonathan Brueggeman, Robert S. Li, Xuehui Oliver, Richard P. McDonald, Bruce A. Friesen, Timothy L. TI Validation of Genome-Wide Association Studies as a Tool to Identify Virulence Factors in Parastagonospora nodorum SO PHYTOPATHOLOGY LA English DT Article ID QUANTITATIVE TRAIT LOCI; STAGONOSPORA-NODORUM; LINKAGE DISEQUILIBRIUM; SACCHAROMYCES-CEREVISIAE; NECROTROPHIC EFFECTORS; SEEDLING RESISTANCE; WHEAT; GENE; BLOTCH; DISCOVERY AB Parastagonospora nodorum is a necrotrophic fungal pathogen causing Septoria nodorum blotch on wheat. We have identified nine necrotrophic effector host dominant sensitivity gene interactions, and we have cloned three of the necrotrophic effector genes, including SnToxA, SnTox1, and SnTox3. Because sexual populations of P. nodorum are difficult to develop under lab conditions, genome-wide association study (GWAS) is the best population genomic approach to identify genomic regions associated with traits using natural populations. In this article, we used a global collection of 191 P. nodorum isolates from which we identified 2,983 single nucleotide polymorphism (SNP) markers and gene markers for SnToxA and SnTox3 to evaluate the power of GWAS on two popular wheat breeding lines that were sensitive to SnToxA and SnTox3. Strong marker trait associations (MTA) with P. nodorum virulence that mapped to SnTox3 and SnToxA were first identified using the marker set described above. A novel locus in the P. nodorum genome associated with virulence was also identified as a result of this analysis. To evaluate whether a sufficient level of marker saturation was available, we designed a set of primers every 1 kb in the genomic regions containing SnToxA and SnTox3. Polymerase chain reaction amplification was performed across the 191 isolates and the presence/absence polymorphism was scored and used as the genotype. The marker proximity necessary to identify MTA flanking SnToxA and SnTox3 ranged from 4 to 5 and 1 to 7 kb, respectively. Similar analysis was performed on the novel locus. Using a 45% missing data threshold, two more SNP were identified spanning a 4.6-kb genomic region at the novel locus. These results showed that the rate of linkage disequilibrium (LD) decay in P. nodorum and, likely, other fungi is high compared with plants and animals. The fast LD decay in P. nodorum is an advantage only if sufficient marker density is attained. Based on our results with the SnToxA and SnTox3 regions, markers are needed every 9 or 8 kb, respectively, or in every gene, to guarantee that genes associated with a quantitative trait such as virulence are not missed. C1 [Gao, Yuanyuan; Liu, Zhaohui; Richards, Jonathan; Brueggeman, Robert S.; Friesen, Timothy L.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Faris, Justin D.; Friesen, Timothy L.] USDA ARS, Northern Crop Sci Lab, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Li, Xuehui] North Dakota State Univ, Dept Plant Sci, Fargo, ND USA. [Oliver, Richard P.] Curtin Univ, Ctr Crop & Dis Management, Dept Environm & Agr, Sch Sci, Perth, WA 6102, Australia. [McDonald, Bruce A.] ETH Zentrum, Swiss Fed Inst Technol, Plant Pathol Grp, Inst Integrat Biol, CH-8092 Zurich, Switzerland. RP Friesen, TL (reprint author), North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA.; Friesen, TL (reprint author), USDA ARS, Northern Crop Sci Lab, Cereal Crops Res Unit, Fargo, ND 58102 USA. EM Timothy.Friesen@ars.usda.gov RI McDonald, Bruce/A-2748-2008 OI McDonald, Bruce/0000-0002-5332-2172 FU United States Department of Agriculture (USDA) National Institute of Food and Agriculture, Agriculture and Food Research Initiative Competitive Grant [2016-67013-24813] FX We thank D. Holmes for technical assistance; C. Cowger, A. Ficke, J. Krupinsky, S. Ali, and P. Lipps for supplying P. nodorum isolates; M. Bolton and S. Solanki for their critical review of the manuscript; and the United States Department of Agriculture (USDA) National Institute of Food and Agriculture, Agriculture and Food Research Initiative Competitive Grant number 2016-67013-24813 for funding. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 49 TC 2 Z9 3 U1 14 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1177 EP 1185 DI 10.1094/PHYTO-02-16-0113-FI PG 9 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100012 ER PT J AU Xiang, C Feng, JY Wang, MN Chen, XM See, DR Wan, AM Wang, T AF Xiang, C. Feng, J. Y. Wang, M. N. Chen, X. M. See, D. R. Wan, A. M. Wang, T. TI Molecular Mapping of Stripe Rust Resistance Gene Yr76 in Winter Club Wheat Cultivar Tyee SO PHYTOPATHOLOGY LA English DT Article DE Triticum aestivum sp compactwn; yellow rust ID F-SP TRITICI; ADULT-PLANT RESISTANCE; MARKER-ASSISTED SELECTION; PUCCINIA-STRIIFORMIS; HIGH-TEMPERATURE; UNITED-STATES; VIRULENCE CHARACTERIZATION; DURABLE RESISTANCE; LINKAGE MAPS; AESTIVUM L. AB Tyee, one of the wheat cultivars used to differentiate races of Puccinia striiformis f. sp. tritici in the United States, was identified to have a single gene for all-stage resistance, tentatively named YrTye. To map the gene, Tyee was crossed with 'Avocet Susceptible' (AvS). Genetic analysis of the F-1, F-2, F-2:3, and BC1 progenies confirmed a single dominant gene for resistance to race PSTv-37 that is avirulent to YrTye. A mapping population of 135 F-2 plants was phenotyped with PSTv-37 and the derived F-2:3 lines were tested with races PSTv-37, PSTv-40, and PSTv-79. The F-2 mapping population was genotyped with simple sequence repeat (SSR) markers. A genetic map comprising 13 SSR markers located YrTye in chromosome 3AS flanked distally by SSR marker wmc11 and proximally by wmc532 at 2.6 and 3.4 cM, respectively. Amplification of Chinese Spring 3A deletion lines placed the gene in the distal bin 3AS4-0.45 to 1.00. Because YrTye is different from all formally named Yr genes in chromosomal location, we permanently name the gene Yr76. A near-isogenic line of spring common wheat was developed and selected by testing F-3 lines derived from a AvS*4/Tyee cross with Tyee-avirulent and virulent races and the flanking markers. The specific SSR alleles flanking Yr76 were validated using cultivars and breeding lines with and without the gene, and showed high polymorphisms. The specificity of Yr76 is useful in differentiating P. striiformis f. sp. tritici races, and its tightly linked markers will be useful in developing resistant cultivars when combining the gene with other genes for resistance to stripe rust. C1 [Xiang, C.; Wang, T.] Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Sichuan, Peoples R China. [Xiang, C.; Feng, J. Y.; Wang, M. N.; Chen, X. M.; See, D. R.; Wan, A. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Feng, J. Y.] Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu 610061, Sichuan, Peoples R China. [Chen, X. M.; See, D. R.] ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. RP Chen, XM (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA.; Chen, XM (reprint author), ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. EM xianming@wsu.edu FU U.S. Department of Agriculture, Agricultural Research Service [5348-22000-015-00D]; Washington State University [13C-3061-5665, 13Z-3061-6665, WNP00663, 99164-6430]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center [PPNS 0710]; China Scholarship Council FX This research was supported by the U.S. Department of Agriculture, Agricultural Research Service (Project 5348-22000-015-00D) and Washington State University (Project 13C-3061-5665 and 13Z-3061-6665) PPNS 0710, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH Project WNP00663, Washington State University, Pullman 99164-6430. The China Scholarship Council scholarships to C. Xiang and J. Feng are highly appreciated. We thank R. McIntosh and K. Garland-Campbell for critical review of the manuscript and R. McIntosh for approving the gene symbol. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 55 TC 2 Z9 2 U1 20 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1186 EP 1193 DI 10.1094/PHYTO-01-16-0045-FI PG 8 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100013 ER PT J AU Bander, BW Zalom, FG Jayanth, M Sudarshana, MR AF Bander, Brian W. Zalom, Frank G. Jayanth, Maya Sudarshana, Mysore R. TI Phylogeny of Geminivirus Coat Protein Sequences and Digital PCR Aid in Identifying Spissistilus festinus as a Vector of Grapevine red blotch-associated virus SO PHYTOPATHOLOGY LA English DT Article DE Erythroneura elegantula; E. variabilis; E. ziczac ID POLYMERASE-CHAIN-REACTION; FAMILY GEMINIVIRIDAE; ALFALFA HOPPER; DNA VIRUS; CALIFORNIA; TRANSMISSION; SPECIFICITY AB Grapevine red blotch-associated virus (GRBaV) is a single-stranded DNA virus, proposed to be a member of the family Geminiviridae, and is associated with grapevines showing red blotch symptoms in North America. The existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. We investigated if a vector exists that can transmit GRBaV in vineyards. Phylogenetic analysis of the predicted amino acid sequence of coat protein (CP) of GRBaV with the CP of 23 geminiviruses representing all seven genera of the family Geminiviridae revealed that GRBaV-CP was most similar to that of Tomato pseudo-curly top virus, a geminivirus known to be transmitted by a treehopper (Membracidae), a family that is closely related to leafhoppers (Cicadellidae). To identify vectors of GRBaV, hemipteran species within and nearby wine grape vineyards where virus spread was suspected were collected and transmission assays were conducted. Among the species tested, the three-cornered alfalfa hopper Spissistilus festinus (Hemiptera: Membracidae) was able to both acquire the virus from a grapevine infected with GRBaV and transmit the virus to healthy grapevines in the laboratory. In commercial vineyards, lateral shoots of grapevines girdled due to feeding injury by the adult three-cornered alfalfa hopper also tested positive for the virus using digital PCR. These findings represent an important step in understanding the biology of GRBaV and develop management guidelines. C1 [Bander, Brian W.; Jayanth, Maya; Sudarshana, Mysore R.] Univ Calif Davis, USDA ARS, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. [Bander, Brian W.; Zalom, Frank G.] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA. RP Sudarshana, MR (reprint author), Univ Calif Davis, USDA ARS, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. EM mysore.sudarshana@ars.usda.gov FU USDA-ARS CRIS [5306-220000-014-00D]; ARS Project, National Plant Disease Recovery System [0500-00082-001-00D]; CDFA Specialty Crops Block Grant Program; California Grapevine Rootstock Improvement Commission; California Tree Fruit and Nut Crops and Grapevine Industry Advisory Board FX This work was supported by USDA-ARS CRIS 5306-220000-014-00D. We thank D. Fravel, National Program Leader-Plant Health, for providing assistance from ARS Project 0500-00082-001-00D, National Plant Disease Recovery System to M. R. Sudarshana. Additional funding for this research was provided by grants from the CDFA Specialty Crops Block Grant Program, California Grapevine Rootstock Improvement Commission, and the California Tree Fruit and Nut Crops and Grapevine Industry Advisory Board to M. R. Sudarshana and F. G. Zalom. We thank J. Jackson, Casa Cristal Nursery, Inc., Delano, CA, for providing potted Cabernet Sauvignon grapevines for the transmission studies; M. Castillo and M. Bollinger for assistance in the field and laboratory; and our collaborators L. Wunderlich, UC Cooperative Extension Central Sierra Region, M. Anderson, UC Davis Department of Viticulture and Enology, and R. Smith, UC Cooperative Extension Sonoma County, for help in identifying vineyards for the study. We appreciate helpful suggestions and comments on the manuscript made by D. Kluepfel, Research Leader, USDA-ARS, Davis, CA. Mention of a trademark, proprietary product, or vendor does not constitute guarantee or warranty of the product by the USDA, and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 26 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD OCT PY 2016 VL 106 IS 10 BP 1223 EP 1230 DI 10.1094/PHYTO-03-16-0125-FI PG 8 WC Plant Sciences SC Plant Sciences GA DW7GV UT WOS:000383820100017 ER PT J AU Kerns, BK Kim, JB Kline, JD Day, MA AF Kerns, Becky K. Kim, John B. Kline, Jeffrey D. Day, Michelle A. TI US exposure to multiple landscape stressors and climate change SO REGIONAL ENVIRONMENTAL CHANGE LA English DT Article DE Environmental and ecological monitoring; Vulnerability and risk assessment; Stressors; Forest health; Wildfire; Insects and disease; Urban and exurban development ID WILDLAND-URBAN INTERFACE; QUANTITATIVE VULNERABILITY ASSESSMENT; UNITED-STATES; SPATIALLY EXPLICIT; WILDFIRE RISK; DISTURBANCE INTERACTIONS; ENVIRONMENTAL-CHANGE; TREE MORTALITY; BARK BEETLES; NEW-MEXICO AB We examined landscape exposure to wildfire potential, insects and disease risk, and urban and exurban development for the conterminous US (CONUS). Our analysis relied on spatial data used by federal agencies to evaluate these stressors nationally. We combined stressor data with a climate change exposure metric to identify when temperature is likely to depart from historical conditions and become "unprecedented." We used a neighborhood analysis procedure based on key stressor thresholds within a geographic information system to examine the extent of landscape exposure to our set of individual and coinciding stressors. Our focus is on identifying large contiguous areas of stress exposure which would be of national concern to identify potential locations most vulnerable to resulting ecological and social disruption. The arrival of record-setting temperatures may be both rapid and widespread within the CONUS under RCP8.5. By 2060, 91 % of the CONUS could depart from the climate of the last century. While much of the CONUS may be impacted by at least one of the landscape stressors we examined, multiple coinciding stressors occurred for less than 9 % of the CONUS. The two most prevalent coinciding stressors were (1) wildfire potential combined with insects and disease risk, and (2) climate departure combined with urban and exurban development. Combined exposure to three or more stressors was rare, but we did identify several localized high-population areas that may be vulnerable to future change. Additional assessment and research for these areas may provide early and proactive approaches to mitigating multiple stressor exposure. C1 [Kerns, Becky K.; Kim, John B.; Kline, Jeffrey D.] US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Day, Michelle A.] Oregon State Univ, Coll Forestry, Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. RP Kerns, BK (reprint author), US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM bkerns@fs.fed.us; jbkim@fs.fed.us; jkline@fs.fed.us; michelle.day@oregonstate.edu FU USDA Forest Service Pacific Northwest Research Station; National Fire Plan FX Climate scenarios used were from the NEX-DCP30 dataset, prepared by the Climate Analytics Group and NASA Ames Research Center using the NASA Earth Exchange and distributed by the NASA Center for Climate Simulation (NCCS). Funding for this research was provided in part by the USDA Forest Service Pacific Northwest Research Station and the National Fire Plan. NR 81 TC 0 Z9 0 U1 12 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-3798 EI 1436-378X J9 REG ENVIRON CHANGE JI Reg. Envir. Chang. PD OCT PY 2016 VL 16 IS 7 SI SI BP 2129 EP 2140 DI 10.1007/s10113-016-0934-2 PG 12 WC Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA DW6OZ UT WOS:000383772100023 ER PT J AU Colliander, A Njoku, EG Jackson, TJ Chazanoff, S McNairn, H Powers, J Cosh, MH AF Colliander, Andreas Njoku, Eni G. Jackson, Thomas J. Chazanoff, Seth McNairn, Heather Powers, Jarrett Cosh, Michael H. TI Retrieving soil moisture for non-forested areas using PALS radiometer measurements in SMAPVEX12 field campaign SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE SMAPVEXI2; PALS; SMAP; Soil moisture ID L-BAND; MICROWAVE EMISSION; WATER-CONTENT; SMAP MISSION; MODEL; ALGORITHM; FREQUENCY; SALINITY; SENSOR; COVER AB In this paper, soil moisture retrievals of surface soil moisture was investigated using L-band brightness temperature under diverse conditions and land cover types. The study focused on the PALS (Passive Active L-band System) radiometer data collected during the SMAPVEXI2 (Soil Moisture Active Passive Validation Experiment 2012) field experiment which took place in southern Manitoba, Canada in 2012. The experiment domain covers croplands with high clay content as well as croplands and grasslands with sandy soils. A retrieval algorithm was parameterized for these specific land types. The formulation of the retrieval algorithm is based on a traditional surface scattering assumption. Based on this data set we found that for the clayey croplands the surface scattering assumption is inadequate, and that the algorithm needed significant tuning for the sandy soils. Empirically-based parameters for retrieving soil moisture under these conditions were developed. We also applied the parameterized algorithm to the retrieval of soil moisture for the entire experiment domain. We found that the use of sub grid modeling improves the retrieval performance to a satisfactory level despite the challenging land types encountered. (C) 2016 Elsevier Inc. All rights reserved. C1 [Colliander, Andreas; Njoku, Eni G.; Chazanoff, Seth] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. [Jackson, Thomas J.; Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [McNairn, Heather; Powers, Jarrett] Agr & Agri Food Canada, Ottawa, ON, Canada. RP Colliander, A (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. EM andreas.colliander@jpl.nasa.gov FU National Aeronautics and Space Administration FX The research described in this publication was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. The SMAPVEX12 experiment data is archived at the National Snow and Ice Data Center (NSIDC), http://nsidc.org/data/smap. NR 66 TC 1 Z9 1 U1 13 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 86 EP 100 DI 10.1016/j.rse.2016.06.001 PG 15 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800007 ER PT J AU Painter, TH Berisford, DF Boardman, JW Bormann, KJ Deems, JS Gehrke, F Hedrick, A Joyce, M Laidlaw, R Marks, D Mattmann, C McGurk, B Ramirez, P Richardson, M Skiles, SM Seidel, FC Winstral, A AF Painter, Thomas H. Berisford, Daniel F. Boardman, Joseph W. Bormann, Kathryn J. Deems, Jeffrey S. Gehrke, Frank Hedrick, Andrew Joyce, Michael Laidlaw, Ross Marks, Danny Mattmann, Chris McGurk, Bruce Ramirez, Paul Richardson, Megan Skiles, S. McKenzie Seidel, Felix C. Winstral, Adam TI The Airborne Snow Observatory: Fusion of scanning lidar, imaging spectrometer, and physically-based modeling for mapping snow water equivalent and snow albedo SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Snow water equivalent; Snow albedo; Water resources; Scanning lidar; Imaging spectrometer ID SEMIARID MOUNTAIN CATCHMENT; WESTERN UNITED-STATES; COLORADO RIVER-BASIN; SIR-C/X-SAR; GRAIN-SIZE; ENERGY-BALANCE; DEPTH MEASUREMENTS; CLIMATE-CHANGE; MASS-BALANCE; COVERED AREA AB Snow cover and its melt dominate regional climate and water resources in many of the world's mountainous regions. Snowmelt timing and magnitude in mountains are controlled predominantly by absorption of solar radiation and the distribution of snow water equivalent (SWE), and yet both of these are very poorly known even in the best-instrumented mountain regions of the globe. Here we describe and present results from the Airborne Snow Observatory (ASO), a coupled imaging spectrometer and scanning lidar, combined with distributed snow modeling, developed for the measurement of snow spectral albedo/broadband albedo and snow depth/SWE. Snow density is simulated over the domain to convert snow depth to SWE. The result presented in this paper is the first operational application of remotely sensed snow albedo and depth/SWE to quantify the volume of water stored in the seasonal snow cover. The weekly values of SWE volume provided by the ASO program represent a critical increase in the information available to hydrologic scientists and resource managers in mountain regions. (C) 2016 Elsevier Inc. All rights reserved. C1 [Painter, Thomas H.; Berisford, Daniel F.; Bormann, Kathryn J.; Joyce, Michael; Laidlaw, Ross; Mattmann, Chris; Ramirez, Paul; Richardson, Megan; Skiles, S. McKenzie; Seidel, Felix C.] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. [Boardman, Joseph W.] Analyt Imaging & Geophys LLC, Boulder, CO USA. [Deems, Jeffrey S.] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Deems, Jeffrey S.] Natl Snow & Ice Data Ctr, Boulder, CO USA. [Gehrke, Frank] Calif Dept Water Resources, Sacramento, CA USA. [Hedrick, Andrew; Marks, Danny; Winstral, Adam] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [McGurk, Bruce] McGurk Hydrol, Orinda, CA USA. RP Painter, TH (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. EM thomas.painter@jpl.nasa.gov RI Painter, Thomas/B-7806-2016; Deems, Jeffrey/E-6484-2016 OI Deems, Jeffrey/0000-0002-3265-8670 FU NASA; California Department of Water Resources; JPL investments; Colorado Water Conservation Board; City of San Francisco Public Utilities Commission; Turlock Irrigation District; Modesto Irrigation District; USDA Agricultural Research Service FX Funding for the Airborne Snow Observatory was provided by NASA, the California Department of Water Resources, JPL investments, Colorado Water Conservation Board, City of San Francisco Public Utilities Commission, Turlock Irrigation District, and Modesto Irrigation District, and USDA Agricultural Research Service. Modeling and density simulations were conducted at the Northwest Watershed Research Center in Boise, Idaho. Part of this work was performed at the Jet Propulsion Laboratory, California Institute of Technology under a contract with NASA. NR 87 TC 7 Z9 7 U1 22 U2 22 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 139 EP 152 DI 10.1016/j.rse.2016.06.018 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800011 ER PT J AU Sulik, JJ Long, DS AF Sulik, John J. Long, Dan S. TI Spectral considerations for modeling yield of canola SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Canola; Brassica oilseed; Yield estimation; Optical remote sensing ID BRASSICA-NAPUS L.; HIGH-TEMPERATURE STRESS; HEAT-STRESS; CROP YIELD; MODIS DATA; RAPE; FLOWER; VEGETATION; NDVI; REFLECTANCE AB Prominent yellow flowers that are present in a Brassica oilseed crop such as canola require careful consideration when selecting a spectral index for yield estimation. This study evaluated spectral indices for multispectral sensors that correlate with the seed yield of Brassica oilseed crops. A small-plot experiment was conducted near Adams, Oregon in which spring canola was grown under varying water regimes and nitrogen treatments to create a wide range in oilseed yield. Plot measurements consisted of canopy reflectance at flowering using a hand-held spectroradiometer and seed yield at physiological maturity. Spectroradiometric measurements were converted to MODIS band equivalent reflectance. Selected indices were computed from spectra obtained with the radiometer and correlated with seed yield. A normalized difference yellowness index (NDYI), computed from the green and blue wavebands, overcame limitations of the normalized difference vegetation index (NDVI) during flowering and best modeled variability in relative yield potential. NDYI was more linear and correlated with county-wide oilseed yield data and MODIS satellite data from North Dakota (r(2) <= 0.72) than NDVI (r(2) <= 0.66). NDYI only requires wavebands in the visible region of the spectrum and can be applied to any satellite or aerial sensor that has blue and green channels. These findings highlight the benefit of using a spectral index that is sensitive to reproductive growth of vegetation instead of vegetative growth for crops with spectrally prominent reproductive canopy elements. Our results indicate that NDYI is a better indicator of yield potential than NDVI during mid-season development stages, especially peak flowering. Published by Elsevier Inc. C1 [Sulik, John J.; Long, Dan S.] USDA ARS, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. RP Sulik, JJ (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM sulikjj@gmail.com; dan.long@ars.usda.gov OI Sulik, John/0000-0003-4637-0886 FU USDA National Institute of Food and Agriculture [2012-10008-19727] FX This research was supported by Research Grant Award No. 2012-10008-19727 from the USDA National Institute of Food and Agriculture. Grateful appreciation to John McCallum, Steve Umbarger, Aron Boettcher, and Mandy Wuest for assistance in the field and laboratory. Use of trade names does not constitute official endorsement by the USDA. NR 49 TC 0 Z9 0 U1 18 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 161 EP 174 DI 10.1016/j.rse.2016.06.016 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800013 ER PT J AU Chen, Q McRoberts, RE Wang, CW Radtke, PJ AF Chen, Qi McRoberts, Ronald E. Wang, Changwei Radtke, Philip J. TI Forest aboveground biomass mapping and estimation across multiple spatial scales using model-based inference SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Biomass; Uncertainty; Lidar; Inventory plots; Destructive tree AGB measurements; Model-based inference ID INDIVIDUAL-TREE VOLUME; NATIONAL FOREST; HEDMARK COUNTY; CARBON SEQUESTRATION; ASSISTED ESTIMATION; MEASUREMENT ERRORS; AIRBORNE LIDAR; INVENTORY; DEFORESTATION; INFORMATION AB Remotely sensed data have been widely used in recent years for mapping and estimating biomass. However, the characterization of the uncertainty of mapped or estimated biomass in previous studies was either based on ad-hoc approaches (e.g., using model fitting statistics such root mean square errors derived from purposive samples) or mostly limited to the analysis of mean biomass for the whole study area. This study proposed a novel uncertainty analysis method that can characterize biomass uncertainty across multiple spatial scales and multiple spatial resolutions. The uncertainty analysis method built on model-based inference and can propagate errors from trees to field plots, individual pixels, and small areas or large regions that consist of multiple pixels (up to all pixels within a study area). We developed and tested this method over northern Minnesota forest areas of approximately 69,508 km(2) via a unique combination of several datasets for biomass mapping and estimation: wall-to-wall airborne lidar data, national forest inventory (NFI) plots, and destructive measurements of tree aboveground biomass (AGB). We found that the pixel-level AGB prediction error is dominated by lidar-based AGB model residual errors when the spatial resolution is near 380 m or finer and by model parameter estimate errors when the spatial resolution is coarser. We also found that the relative error of AGB predicted from lidar can be reduced to approximately 11% (or mean 5.1 Mg/ha; max 43.6 Mg/ha) at one-hectare scale (or at 100 m spatial resolution) over our study area. Because our uncertainty analysis method uses model-based inference and does not require probability samples of field plots, our methodology has potential applications worldwide, especially over tropics and developing countries where NFI systems are not well-established. (C) 2016 Elsevier Inc. All rights reserved. C1 [Chen, Qi; Wang, Changwei] Univ Hawaii Manoa, Dept Geog, 422 Saunders Hall,2424 Maile Way, Honolulu, HI USA. [McRoberts, Ronald E.] US Forest Serv, Northern Res Stn, St Paul, MN USA. [Wang, Changwei] South China Agr Univ, Coll Nat Resources & Environm, Guangzhou, Guangdong, Peoples R China. [Radtke, Philip J.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. RP Chen, Q (reprint author), Univ Hawaii Manoa, Dept Geog, 422 Saunders Hall,2424 Maile Way, Honolulu, HI USA. EM qichen@hawaii.edu FU College of Social Sciences (CSS) Research Support Award at the University of Hawaii, Manoa; science and technology project of Guangdong Province [2013B020314016] FX This research has been supported by the College of Social Sciences (CSS) Research Support Award at the University of Hawaii, Manoa and the science and technology project of Guangdong Province (2013B020314016). NR 60 TC 3 Z9 3 U1 22 U2 22 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 350 EP 360 DI 10.1016/j.rse.2016.07.023 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800027 ER PT J AU McRoberts, RE Domke, GM Chen, Q Naesset, E Gobakken, T AF McRoberts, Ronald E. Domke, Grant M. Chen, Qi Naesset, Erik Gobakken, Terje TI Using genetic algorithms to optimize k-Nearest Neighbors configurations for use with airborne laser scanning data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Inference; Spatial estimation; National forest inventory ID ESTIMATING BASAL AREA; FOREST VARIABLES; BIOMASS; LIDAR; VOLUME; INFERENCE; ATTRIBUTES; PREDICTION; INTENSITY AB The relatively small sampling intensities used by national forest inventories are often insufficient to produce the desired precision for estimates of population parameters unless the estimation process is augmented with auxiliary information, usually in the form of remotely sensed data. The k-Nearest Neighbors (k-NN) technique is a non-parametric, multivariate approach to prediction that has emerged as particularly popular for use with forest inventory and remotely sensed data and has been shown to contribute substantially to increasing precision. k-NN predictions are calculated as linear combinations of observations for sample units that are nearest in a space of auxiliary variables to the population unit for which a prediction is desired. Implementation of a nearest neighbors algorithm requires four choices: (i) a distance metric, (ii) specific auxiliary variables to be used with the distance metric, (iii) the number of nearest neighbors, and a (iv) scheme for weighting the nearest neighbors. Regardless of the choices for a distance metric and weighting scheme, emerging evidence suggests that optimization of the technique, including selection of an optimal subset of auxiliary variables, greatly enhances prediction. However, optimization can be computationally intensive and time-consuming. A promising approach that is gaining favor is based on genetic algorithms, a technique that uses search heuristics that mimic natural selection to solve optimization problems. The objective of the study was to compare optimized k-NN configurations with respect to inferences for mean volume per unit area using airborne laser scanning variables as auxiliary information. For two study areas, one in Norway and one in Minnesota, USA, the analyses focused on optimizing k-NN configurations that used the weighted Euclidean and canonical correlation distance metrics and two neighbor weighting schemes. Novel features of the study include introduction of a neighbor weighting scheme that has not previously been used for forestry applications, simultaneous optimization of all four k-NN choices, and basing comparisons on confidence intervals, rather than intermediate products such as prediction accuracies. Two conclusions were primary: (1) optimized selection of feature variables produced greater precision than using all feature variables, and (2) computational intensity necessary to optimize the weighted Euclidean metric was considerably greater than for the canonical correlation analysis metric. Specific findings were that optimization produced pseudo-R-2 as large as 0.87 for the Norwegian dataset and as large as 0.89 for the Minnesota dataset. For the optimized canonical correlation distance metric, widths of approximate 95% confidence intervals as proportions of the estimated means were as small as 0.13 for the Norwegian dataset and as small as 0.15 for the Minnesota dataset. Published by Elsevier Inc. C1 [McRoberts, Ronald E.; Domke, Grant M.] US Forest Serv, Northern Res Stn, St Paul, MN 55108 USA. [Chen, Qi] Univ Hawaii Manoa, Dept Geog, Honolulu, HI 96822 USA. [Naesset, Erik; Gobakken, Terje] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, As, Norway. RP McRoberts, RE (reprint author), US Forest Serv, Northern Res Stn, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us OI Domke, Grant/0000-0003-0485-0355 NR 42 TC 0 Z9 0 U1 8 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 387 EP 395 DI 10.1016/j.rse.2016.07.007 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800030 ER PT J AU Koltunov, A Ustin, SL Quayle, B Schwind, B Ambrosia, VG Li, W AF Koltunov, Alexander Ustin, Susan L. Quayle, Brad Schwind, Brian Ambrosia, Vincent G. Li, Wei TI The development and first validation of the GOES Early Fire Detection (GOES-EFD) algorithm SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Geostationary; Satellite; Fire detection; Wildfire detection; Early wildfire detection; Wildfire; Detection timeliness; Fire monitoring; Remote sensing; GOES Early Fire Detection; GOES-EFD ID PRODUCT; SOUTH; AMERICA; IMAGERY; MODIS AB Decades of successful active fire mapping from space, have led to global informational products of growing importance to scientific community and operational agencies. In contrast, detecting fires from space faster than current conventional capabilities in the continental U.S. has not been considered attainable, except in remote, sparsely populated areas. We present a research prototype version of the GOES Early Fire Detection (GOES-EFD) algorithm focused on minimizing the time to first detection of a wildfire incident. The algorithm is designed for regional-scale surveillance and combines multitemporal anomaly tests developed in our previous work, contextual hot-spot tests, and dynamic event classification and tracking. The GOES-EFD version 0.4 was initially tested with 40-day summer 2006 data over central California. The algorithm identified most of large (final size> 2 ha) wildfires within 30 min and 31% of the wildfires were detected before they were reported by the public. Under identical operation conditions, GOES-EFD 0.4 provided quicker initial detection than the temporally filtered operational WF-ABBA algorithm (version 6.1) and committed fewer false alarms. There is a substantial potential for further reducing detection latency and increasing reliability. Following the ongoing optimizations, tests, and integration in collaboration with the fire management agencies and first responders, GOES-EFD could be deployed for regional scale real-time surveillance to complement existing fire identification methods. (C) 2016 Elsevier Inc. All rights reserved. C1 [Koltunov, Alexander; Ustin, Susan L.; Li, Wei] Univ Calif Davis, Ctr Spatial Technol & Remote Sensing, Veihmeyer Hall,One Shields Ave, Davis, CA 95616 USA. [Quayle, Brad; Schwind, Brian] Forest Serv, USDA, RSAC, 2222 West 2300 South, Salt Lake City, UT 84119 USA. [Ambrosia, Vincent G.] Calif State Univ Monterey Bay, NASA, Ames Res Ctr, Moffett Field, CA 94035 USA. [Li, Wei] Beijing Univ Chem Technol, Coll Informat Sci & Technol, Beijing 100029, Peoples R China. RP Koltunov, A (reprint author), Univ Calif Davis, Ctr Spatial Technol & Remote Sensing, Veihmeyer Hall,One Shields Ave, Davis, CA 95616 USA. EM akoltunov@ucdavis.edu FU US Forest Service; University of California, Davis "Evaluating operational potential of geostationary early fire detection capabilities at regional level" [10-IA-11130400-009]; U.S. Department of Homeland Security [RSID-11-00096, HSHQDC-11-C-00158] FX This work was supported by US Forest Service, as the main sponsor, and University of California, Davis under Cost Share Agreement 10-IA-11130400-009 "Evaluating operational potential of geostationary early fire detection capabilities at regional level", with contribution by the U.S. Department of Homeland Security (Project No. RSID-11-00096; Contract No. HSHQDC-11-C-00158). We thank Ahmad Hakim-Elahi (UC Davis, Sponsored Program Office) and Bruce A. Davis (Dept. Homeland Security, Science & Technology Directorate) for their dedication to support GOES-EFD development. The GOES Imager data readout capability and technical assistance was provided by the CIMIS (California Irrigation Management Information System) program. We also thank Quinn Hart (UC Davis) for his assistance and advice that helped us preprocess GOES GVAR data; Mark Rosenberg (California Department of Forestry and Fire Protection, CAL FIRE) for providing geospatial wildfire data, support, and useful discussions; Mui Lay (UC Davis) for her great help with Landsat image interpretation; George Scheer (UC Davis) for computation and data management support; Mark Finco and Linda R. Smith (RedCastle Resources, Inc. /USDA Forest Service, RSAC) for assistance with graphics design; Mark Ruminski (NOAA, NESDIS) for helpful discussions of the algorithm. We are grateful to Elaine Prins (UW-Madison, CIMSS) for her highly valuable insights into operational geostationary wildfire detection and aspects of WF-ABBA algorithm, and thank two anonymous reviewers for useful suggestions and constructive criticism that significantly improved the manuscript. NR 40 TC 0 Z9 0 U1 10 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 436 EP 453 DI 10.1016/j.rse.2016.07.021 PG 18 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800034 ER PT J AU Hall, JV Loboda, TV Giglio, L McCarty, GW AF Hall, Joanne V. Loboda, Tatiana V. Giglio, Louis McCarty, Gregory W. TI A MODIS-based burned area assessment for Russian croplands: Mapping requirements and challenges SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Burned area; Cropland residue burning; Russian Federation; Remote sensing; MODIS ID IMAGING-SPECTRORADIOMETER MODIS; AGRICULTURAL FIRES; AIR-POLLUTION; BLACK CARBON; ALGORITHM; RESOLUTION; FEDERATION; EMISSIONS; IMPACTS; EUROPE AB Although agricultural burning is banned in Russia, it is still a widespread practice. Accurately monitoring cropland burned area is an important task as these estimates are used in the calculation of cropland burning emissions, which are ultimately utilized in policy making decisions. In this paper we developed an independent estimate of cropland burning in Russia through assessing the capabilities of global burned area products (MCD45A1; Roy et al., 2008 and MCD64A1; Giglio et al., 2009) and our own custom Moderate Resolution Imaging Spectroradiometer (MODIS) based Cropland Regional Area Burned (CRAB) product. An archive of cropland field state samples was generated from very high resolution (<5 m) imagery allowing us a unique perspective into the challenges of mapping cropland burned area through detailed analysis of the Russian agricultural practices. Our analysis showed all three burned area products were unable to map approximately 95% of burn validation samples, demonstrating that the current coarse resolution (defined here as >= 500 m) satellite capabilities are not adequate for accurately mapping burned area associated with agricultural fires. The transient nature of cropland burns and the climatological conditions of the region require multiple subsequent daily overpasses; however, the small spatial scale of the burns requires moderate (defined here as 10-50 m) spatial resolutions. Future opportunities to accurately map cropland burned area may arise with the Sentinel-2 and Landsat constellation missions. (C) 2016 The Authors. Published by Elsevier Inc. C1 [Hall, Joanne V.; Loboda, Tatiana V.; Giglio, Louis] Univ Maryland, Dept Geog Sci, 2181 Samuel J LeFrak Hall,7251 Preinkert Dr, College Pk, MD 20742 USA. [McCarty, Gregory W.] Beltsville Agr Res Serv, USDA, 10300 Baltimore Ave 302, Beltsville, MD 20705 USA. RP Hall, JV (reprint author), Univ Maryland, Dept Geog Sci, 2181 Samuel J LeFrak Hall,7251 Preinkert Dr, College Pk, MD 20742 USA. EM jhall1@umd.edu FU U.S. Department of Agriculture: Black Carbon Initiative - U.S. Department of State [S-OES-10-IAA-0025] FX This project was supported by the U.S. Department of Agriculture: Black Carbon Initiative funded by a grant from the U.S. Department of State (S-OES-10-IAA-0025) under the oversight of Mr. B Kinder (USDA Forest Service). The authors would like to thank the National Geospatial Intelligence Agency for providing us with the very high resolution imagery and the image analysts; K. Ageh, R. Crawford, A. Culp-Cano, A. Hoffman-Hall, P. McDonough and, L. Wang from the University of Maryland and S. Devereaux from the USDA ARS for assisting with data download and digitizing the VHR archive. NR 55 TC 0 Z9 0 U1 12 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 506 EP 521 DI 10.1016/j.rse.2016.07.022 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800039 ER PT J AU Kustas, WP Nieto, H Morillas, L Anderson, MC Alfieri, JG Hipps, LE Villagarcia, L Domingo, F Garcia, M AF Kustas, William P. Nieto, Hector Morillas, Laura Anderson, Martha C. Alfieri, Joseph G. Hipps, Lawrence E. Villagarcia, Luis Domingo, Francisco Garcia, Monica TI Revisiting the paper "Using radiometric surface temperature for surface energy flux estimation in Mediterranean drylands from a two-source perspective" SO REMOTE SENSING OF ENVIRONMENT LA English DT Article ID SENSIBLE HEAT-FLUX; SPARSE SAHELIAN VEGETATION; DUAL ANGLE OBSERVATIONS; PARTIAL CANOPY COVER; BALANCE MODEL; KB(-1) PARAMETER; ROUGHNESS HEIGHT; PRIESTLEY-TAYLOR; SEMIARID AREAS; VAPOR TRANSFER AB The recent paper by Morillas et al. [Morillas, L et al. Using radiometric surface temperature for surface energy flux estimation in Mediterranean drylands from a two-source perspective, Remote Sens. Environ. 136, 234-246, 2013] evaluates the two-source model (TSM) of Norman et al. (1995) with revisions by Kustas and Norman (1999) over a semiarid tussock grassland site in southeastern Spain. The TSM - in its current incarnation, the two-source energy balance model (TSEB) - was applied to this landscape using ground-based infrared radiometer sensors to estimate both the composite surface radiometric temperature and component soil and canopy temperatures. Morillas et al. (2013) found the TSEB model substantially underestimated the sensible H (and overestimated the latent heat LE) fluxes. Using the same data set from Morillas et al. (2013), we were able to confirm their results. We also found energy transport and exchange behavior derived from primarily the observations themselves to differ significantly from a number of prior studies using land surface temperature for estimating heat fluxes with one-source modeling approaches in semi-arid landscapes. However, revisions to key vegetation inputs to TSEB and the soil resistance formulation resulted in a significant reduction in the bias and root mean square error (RMSE) between model output of H and LE and the measurements compared to the prior results from Morillas et al. (2013). These included more representative ground-based vegetation greenness and local leaf area index values as well as modifications to the coefficients of the soil resistance formulation to account for the very rough (rocky) soil surface conditions with a clumped canopy. This indicates that both limitations in remote estimates of biophysical indicators of the canopy at the site and the lack of adjustment in soil resistance formulation to account for site specific characteristics, contributed to the earlier findings of Morillas et al. (2013). This suggests further studies need to be conducted to reduce the uncertainties in the vegetation and land surface temperature input data in order to more accurately assess the effects of the transport exchange processes of this Mediterranean landscape on TSEB formulations. Published by Elsevier Inc. C1 [Kustas, William P.; Anderson, Martha C.; Alfieri, Joseph G.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Nieto, Hector] Spanish Res Council, Inst Sustainable Agr, Cordoba, Spain. [Morillas, Laura] Univ British Columbia, Dept Earth Ocean & Atmospher Sci, Vancouver, BC, Canada. [Hipps, Lawrence E.] Utah State Univ, Dept Biometeorol & Climatol, Logan, UT 84322 USA. [Villagarcia, Luis] Univ Pablo de Olavide, Dept Phys Chem & Nat Syst, ES-1013 Seville, Spain. [Domingo, Francisco] CSIC, EEZA, Almeria, Spain. [Garcia, Monica] Tech Univ Denmark, Dept Environm Engn, Lyngby, Denmark. [Garcia, Monica] Columbia Univ, Int Res Inst Climate & Soc IRI, New York, NY 10027 USA. RP Kustas, WP (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM Bill.Kustas@ars.usda.gov RI Garcia, Monica/N-1206-2014; Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU Andalusia Regional Government projects [P06-RNM-01732, P08-RNM-3721]; European Union (ERDF funds); Spanish Ministry of Science and Innovation [CGL2011-27493]; Danish Council for Independent Research \ Technology and Production Sciences [09-070382] FX Dr. Laura Morillas, Professor Monica Garcia, Dr. Luis Villagarcia and Dr. Francisco Domingo would like to thank funding by Andalusia Regional Government projects (P06-RNM-01732, P08-RNM-3721), European Union (ERDF funds) with support from the Spanish Ministry of Science and Innovation (CGL2011-27493) and the Danish Council for Independent Research vertical bar Technology and Production Sciences (Grant 09-070382) USDA is an equal opportunity provider and employer. NR 46 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD OCT PY 2016 VL 184 BP 645 EP 653 DI 10.1016/j.rse.2016.07.024 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DW7JU UT WOS:000383827800048 ER PT J AU Dutta, T Kim, KH Uchimiya, M Kwon, EE Jeon, BH Deep, A Yun, ST AF Dutta, Tanushree Kim, Ki-Hyun Uchimiya, Minori Kwon, Eilhann E. Jeon, Byong-Hun Deep, Akash Yun, Seong-Taek TI Global demand for rare earth resources and strategies for green mining SO ENVIRONMENTAL RESEARCH LA English DT Review DE Rare earth ore deposits; Ion-adsorption; Urban mining; Preservation; Recycling ID SEDIMENTARY PHOSPHATE DEPOSITS; BREAKDOWN SPECTROSCOPY LIBS; SAN-FRANCISCO BAY; PROCESS OPTIMIZATION; SOLVENT-EXTRACTION; FLUORESCENT LAMPS; FLY-ASH; ELEMENTS; RECOVERY; METALS AB Rare earth elements (REEs) are essential raw materials for emerging renewable energy resources and 'smart' electronic devices. Global REE demand is slated to grow at an annual rate of 5% by 2020. This high growth rate will require a steady supply base of REEs in the long run. At present, China is responsible for 85% of global rare earth oxide (REO) production. To overcome this monopolistic supply situation, new strategies and investments are necessary to satisfy domestic supply demands. Concurrently, environmental, economic, and social problems arising from REE mining must be addressed. There is an urgent need to develop efficient REE recycling techniques from end-of-life products, technologies to minimize the amount of REEs required per unit device, and methods to recover them from fly ash or fossil fuel burning wastes. (C) 2016 Elsevier Inc. All rights reserved. C1 [Dutta, Tanushree; Kim, Ki-Hyun] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. [Uchimiya, Minori] USDA ARS, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Kwon, Eilhann E.] Sejong Univ, Environm & Energy Dept, Seoul 05006, South Korea. [Jeon, Byong-Hun] Hanyang Univ, Dept Nat Resources & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. [Deep, Akash] CSIR, CSIO, Sect 30C, Chandigarh 160030, India. [Yun, Seong-Taek] Korea Univ, Dept Earth & Environm Sci, Seoul 02841, South Korea. [Yun, Seong-Taek] Korea Univ, KU KIST Green Sch, Seoul 02841, South Korea. RP Kim, KH (reprint author), Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea. EM kkim61@hanyang.ac.kr FU National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) [2009-0093848]; National Research Foundation of Korea (NRF) Grant - Korean Government (MSIP) [2914RA1A004893] FX This study was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (No. 2009-0093848). E Kwon also acknowledges the support made by a National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. 2914RA1A004893). NR 75 TC 5 Z9 5 U1 66 U2 66 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0013-9351 EI 1096-0953 J9 ENVIRON RES JI Environ. Res. PD OCT PY 2016 VL 150 BP 182 EP 190 DI 10.1016/j.envres.2016.05.052 PG 9 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA DV4NU UT WOS:000382903100024 PM 27295408 ER PT J AU Wang, JM Zhuang, H Zhang, JH AF Wang, Jiamei Zhuang, Hong Zhang, Jianhao TI Inactivation of Spoilage Bacteria in Package by Dielectric Barrier Discharge Atmospheric Cold Plasma-Treatment Time Effects SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Non-thermal plasma; Ozone; pH; Psuedomonas; Macrococcus ID SYSTEM AB The objective was to investigate the effect of treatment time of dielectric barrier discharge atmospheric cold plasma (DBD-ACP) on inactivation of spoilage bacteria, Pseudomonas fluorescens and Macrococcus caseolyticus. P. fluorescens and M. caseolyticus were isolated from spoiled chicken carcasses and suspended in liquid media in packages before the samples were treated with DBD-ACP at 55 kV for different times. Ozone concentrations in package headspace and pH changes in bacterial suspensions were measured to assess plasma formation and antimicrobial activity inside packages. Colony formation was used to indicate the inactivation efficacy. Ozone concentrations reached 1000 ppm after a 3-min treatment. pH dropped by more than 0.25 units in bacterial suspensions immediately after a 6-min treatment and 0.70 units after the 6-min treatment and 24-h storage at 4 A degrees C. Populations of both bacteria significantly reduced as the DBD-ACP treatment time increased. The treatment for less than 1.5 min resulted in more than 1 log reductions in both bacteria, which were similar to each other. However, treatments for additional 1.5 min reduced P. fluorescens and M. caseolyticus populations by more than 4 and 2.5 log cycles, respectively. The difference in bacterial kills between the two types of bacteria extended to the 6-min treatments. Our results demonstrate that treatment times significantly affect the inactivation of spoilage bacteria by DBD-ACP system. After the treatment times are extended for more than 1.5 min, the in-package DBD-ACP treatment has more effects on Gram-negative spoilage bacteria P. fluorescens than Gram-positive spoilage bacteria M. caseolyticus. C1 [Wang, Jiamei] Hainan Univ, Coll Food Sci & Technol, Haikou 570228, Peoples R China. [Wang, Jiamei; Zhang, Jianhao] Nanjing Agr Univ, Coll Food Sci & Technol, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Zhuang, Hong] USDA ARS, US Natl Poultry Res Ctr, Athens, GA 30605 USA. RP Zhang, JH (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China.; Zhuang, H (reprint author), USDA ARS, US Natl Poultry Res Ctr, Athens, GA 30605 USA. EM hong.zhuang@ars.usda.gov; nau_zjh@njau.edu.cn FU Agriculture Science and Technology Innovation Fund of Jiangsu province; Scientific Research Funding of Hainan University FX The authors are grateful to Hinton Arthur Jr., Nicole Bartenfield, and Kimberly Ingram, US National Poultry Research Center, USDA-ARS, Athens, GA 30605, for their help with bacteria measure and technology support. The research has received funding from Agriculture Science and Technology Innovation Fund of Jiangsu province and Scientific Research Funding of Hainan University. NR 13 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD OCT PY 2016 VL 9 IS 10 BP 1648 EP 1652 DI 10.1007/s11947-016-1746-6 PG 5 WC Food Science & Technology SC Food Science & Technology GA DV5MR UT WOS:000382972000003 ER PT J AU Johnson, DM AF Johnson, David M. TI A comprehensive assessment of the correlations between field crop yields and commonly used MODIS products SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article ID LAND-SURFACE TEMPERATURE; REMOTE-SENSING DATA; WHEAT YIELD; VEGETATION INDEXES; GRAIN-YIELD; ESTIMATION MODEL; SPECTRAL DATA; GREAT-PLAINS; NDVI DATA; PREDICTION AB An exploratory assessment was undertaken to determine the correlation strength and optimal timing of several commonly used Moderate Resolution Imaging Spectroradiometer (MODIS) composited imagery products against crop yields for 10 globally significant agricultural commodities. The crops analyzed included barley, canola, corn, cotton, potatoes, rice, sorghum, soybeans, sugarbeets, and wheat. The MODIS data investigated included the Normalized Difference Vegetation Index (NDVI), Fraction of Photosynthetically Active Radiation (FPAR), Leaf Area Index (LAI), and Gross Primary Production (GPP), in addition to daytime Land Surface Temperature (DLST) and nighttime LST (NLST). The imagery utilized all had 8-day time intervals, but NDVI had a 250 m spatial resolution while the other products were 1000 m. These MODIS datasets were also assessed from both the Terra and Aqua satellites, with their differing overpass times, to document any differences. A follow-on analysis, using the Terra 250 m NDVI data as a benchmark, looked at the yield prediction utility of NDVI at two spatial scales (250 m vs. 1000 m), two time precisions (8-day vs. 16-day), and also assessed the Enhanced Vegetation Index (EVI, at 250 m, 16-day). The analyses spanned the major farming areas of the United States (US) from the summers of 2008-2013 and used annual county-level average crop yield data from the US Department of Agriculture as a basis. All crops, except rice, showed at least some positive correlations to each of the vegetation related indices in the middle of the growing season, with NDVI performing slightly better than FPAR. LAI was somewhat less strongly correlated and GPP weak overall. Conversely, some of the crops, particularly canola, corn, and soybeans, also showed negative correlations to DLST mid-summer. NLST, however, was never correlated to crop yield, regardless of the crop or seasonal timing. Differences between the Terra and Aqua results were found to be minimal. The 1000 m resolution NDVI showed somewhat poorer performance than the 250 m and suggests spatial resolution is helpful but not a necessity. The 8-day versus 16-day NDVI relationships to yields were very similar other than for the temporal precision. Finally, the EVI often showed the very best performance of all the variables, all things considered. Published by Elsevier B.V. C1 [Johnson, David M.] Natl Agr Stat Serv, USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. RP Johnson, DM (reprint author), Natl Agr Stat Serv, USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. EM dave.johnson@nass.usda.gov NR 56 TC 0 Z9 0 U1 35 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD OCT PY 2016 VL 52 BP 65 EP 81 DI 10.1016/j.jag.2016.05.010 PG 17 WC Remote Sensing SC Remote Sensing GA DV5XH UT WOS:000383003500007 ER PT J AU Coopersmith, EJ Cosh, MH Bell, JE Kelly, V Hall, M Palecki, MA Temimi, M AF Coopersmith, Evan J. Cosh, Michael H. Bell, Jesse E. Kelly, Victoria Hall, Mark Palecki, Michael A. Temimi, Marouane TI Deploying temporary networks for upscaling of sparse network stations SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Soil moisture; Temporary networks; In-situ observation; Upscaling; Data mining ID CLIMATE REFERENCE NETWORK; SOIL-MOISTURE; STABILITY AB Soil observations networks at the national scale play an integral role in hydrologic modeling, drought assessment, agricultural decision support, and our ability to understand climate change. Understanding soil moisture variability is necessary to apply these measurements to model calibration, business and consumer applications, or even human health issues. The installation of soil moisture sensors as sparse, national networks is necessitated by limited financial resources. However, this results in the incomplete sampling of the local heterogeneity of soil type, vegetation cover, topography, and the fine spatial distribution of precipitation events. To this end, temporary networks can be installed in the areas surrounding a permanent installation within a sparse network. The temporary networks deployed in this study provide a more representative average at the 3 km and 9 km scales, localized about the permanent gauge. The value of such temporary networks is demonstrated at test sites in Millbrook, New York and Crossville, Tennessee. The capacity of a single U.S. Climate Reference Network (USCRN) sensor set to approximate the average of a temporary network at the 3 km and 9 km scales using a simple linear scaling function is tested. The capacity of a temporary network to provide reliable estimates with diminishing numbers of sensors, the temporal stability of those networks, and ultimately, the relationship of the variability of those networks to soil moisture conditions at the permanent sensor are investigated. In this manner, this work demonstrates the single-season installation of a temporary network as a mechanism to characterize the soil moisture variability at a permanent gauge within a sparse network. (C) 2016 Elsevier B.V. All rights reserved. C1 [Coopersmith, Evan J.; Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Bell, Jesse E.] Cooperat Inst Climate & Satellites NC, Asheville, NC 28801 USA. [Kelly, Victoria] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Hall, Mark] Oak Ridge Associated Univ, NOAA ATDD, Oak Ridge, TN 37830 USA. [Bell, Jesse E.; Palecki, Michael A.] NOAA, Natl Ctr Environm Informat, Asheville, NC 28801 USA. [Temimi, Marouane] CUNY, New York, NY 10021 USA. [Temimi, Marouane] Masdar Inst Sci & Technol, Abu Dhabi, U Arab Emirates. RP Coopersmith, EJ (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. EM evan@prognosticdatasolutions.com OI Palecki, Michael/0000-0002-1557-9866; Coopersmith, Evan/0000-0002-6223-4828 FU NOAA through the Cooperative Institute for Climate and Satellites - North Carolina [NA09NES4400006]; NASA Terrestrial Hydrology Program [NNH10ZDA001N-THP]; USDA Agricultural Research Service FX This work was supported by NOAA through the Cooperative Institute for Climate and Satellites - North Carolina under Cooperative Agreement NA09NES4400006. This work was also supported by the NASA Terrestrial Hydrology Program (NNH10ZDA001N-THP) and USDA Agricultural Research Service. USDA is an equal opportunity provider and employer. Additional thanks are owed to Howard Diamond and NOAA's Atmospheric Turbulence and Diffusion Division (ATDD). NR 29 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD OCT PY 2016 VL 52 BP 433 EP 444 DI 10.1016/j.jag.2016.07.013 PG 12 WC Remote Sensing SC Remote Sensing GA DV5XH UT WOS:000383003500040 ER EF