FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Vieira, P Eves-van den Akker, S Verma, R Wantoch, S Eisenback, JD Kamo, K AF Vieira, Paulo Eves-van den Akker, Sebastian Verma, Ruchi Wantoch, Sarah Eisenback, Jonathan D. Kamo, Kathryn TI The Pratylenchus penetrans Transcriptome as a Source for the Development of Alternative Control Strategies: Mining for Putative Genes Involved in Parasitism and Evaluation of in planta RNAi SO PLOS ONE LA English DT Article ID ROOT-KNOT NEMATODE; CYST-NEMATODE; MELOIDOGYNE-INCOGNITA; LESION NEMATODE; FUNCTIONAL CLASSIFICATION; CAENORHABDITIS-ELEGANS; EXPRESSION PATTERN; RADOPHOLUS-SIMILIS; GLOBODERA-PALLIDA; REFERENCE GENOME AB The root lesion nematode Pratylenchus penetrans is considered one of the most economically important species within the genus. Host range studies have shown that nearly 400 plant species can be parasitized by this species. To obtain insight into the transcriptome of this migratory plant-parasitic nematode, we used Illumina mRNA sequencing analysis of a mixed population, as well as nematode reads detected in infected soybean roots 3 and 7 days after nematode infection. Over 140 million paired end reads were obtained for this species, and de novo assembly resulted in a total of 23,715 transcripts. Homology searches showed significant hit matches to 58% of the total number of transcripts using different protein and EST databases. In general, the transcriptome of P. penetrans follows common features reported for other root lesion nematode species. We also explored the efficacy of RNAi, delivered from the host, as a strategy to control P. penetrans, by targeted knock-down of selected nematode genes. Different comparisons were performed to identify putative nematode genes with a role in parasitism, resulting in the identification of transcripts with similarities to other nematode parasitism genes. Focusing on the predicted nematode secreted proteins found in this transcriptome, we observed specific members to be up-regulated at the early time points of infection. In the present study, we observed an enrichment of predicted secreted proteins along the early time points of parasitism by this species, with a significant number being pioneer candidate genes. A representative set of genes examined using RT-PCR confirms their expression during the host infection. The expression patterns of the different candidate genes raise the possibility that they might be involved in critical steps of P. penetrans parasitism. This analysis sheds light on the transcriptional changes that accompany plant infection by P. penetrans, and will aid in identifying potential gene targets for selection and use to design effective control strategies against root lesion nematodes. C1 [Vieira, Paulo; Eisenback, Jonathan D.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Vieira, Paulo; Verma, Ruchi; Wantoch, Sarah; Kamo, Kathryn] US Natl Arboretum, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD 20705 USA. [Eves-van den Akker, Sebastian] Univ Dundee, Sch Life Sci, Dundee DD1 5EH, Scotland. RP Vieira, P (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM pvieira@vt.edu OI Vieira, Paulo/0000-0001-5627-2628 FU National Institute of Food and Agriculture, U.S. Department of Agriculture [11588909]; Fred C. Gloeckner Foundation; California Department of Food and Agriculture Specialty Crops Block Grant FX This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 11588909. This work was also partially funded by the Fred C. Gloeckner Foundation and the California Department of Food and Agriculture 2012 Specialty Crops Block Grant.; We thank Dr. David Chitwood (Nematology Laboratory, USDA, Beltsville, MD) for providing the Pratylenchus penetrans isolate, Dr. Benjamin Matthews (Soybean Genomics and Improvement Laboratory, USDA, Beltsville, MD) for providing the pRAP17 vector and the Agrobacterium tumefaciens strain K599. We thank Prof. Godelieve Gheysen, Prof. Mike Jones and Dr. John Fosu-Nyarko for providing the transcriptome assembly of P. coffeae and P. zeae, respectively. This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 11588909. This work was also partially funded by the Fred C. Gloeckner Foundation and the California Department of Food and Agriculture 2012 Specialty Crops Block Grant. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 82 TC 2 Z9 2 U1 3 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 14 PY 2015 VL 10 IS 12 AR e0144674 DI 10.1371/journal.pone.0144674 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CY9GR UT WOS:000366715900085 PM 26658731 ER PT J AU Appell, M Jackson, MA Wang, LJC Bosma, WB AF Appell, Michael Jackson, Michael A. Wang, Lijuan C. Bosma, Wayne B. TI Determination of Citrinin Using Molecularly Imprinted Solid Phase Extraction Purification, HPLC Separation, and Fluorescence Detection SO JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES LA English DT Article DE food safety; HPLC; MIP; MISPE; molecularly imprinted polymer; mycotoxin ID RED YEAST RICE; MYCOTOXIN CITRININ; IN-VITRO; OCHRATOXIN; PRODUCTS; CHROMATOGRAPHY; COMBINATION; RESIDUES; POLYMERS; CLEANUP AB A liquid chromatography based method to detect citrinin in corn was developed using molecularly imprinted solid phase extraction (MISPE) sample clean-up. Molecularly imprinted polymers were synthesized using 1,4-dihydroxy-2-naphthoic acid as the template and an amine functional monomer. Density functional calculations suggest the mimic template interacts with the functional monomer in a similar manner as citrinin. Freundlich isotherm analysis indicated the template provided a significant imprinting effect for citrinin binding. A high performance liquid chromatography with fluorescence detection (HPLC-FD) method to detect citrinin in maize was developed utilizing the imprinted polymers for sample clean-up (excitation at 330nm; emission at 500nm). Recoveries of citrinin in spiked corn samples (0.03-3 mu gg(-1)) were between 82.3-91.5%. This study demonstrates that molecularly imprinted polymers are applicable in the solid phase extraction clean-up of corn samples for citrinin determination by HPLC-FD. C1 [Appell, Michael; Wang, Lijuan C.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Jackson, Michael A.] ARS, Renewable Prod Technol Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Bosma, Wayne B.] Bradley Univ, Mund Lagowski Dept Chem & Biochem, Peoria, IL 61625 USA. RP Appell, M (reprint author), USDA ARS NCAUR, Mycotoxin Prevent & Appl Microbiol Res, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.appell@ars.usda.gov NR 26 TC 1 Z9 1 U1 6 U2 20 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1082-6076 EI 1520-572X J9 J LIQ CHROMATOGR R T JI J. Liq. Chromatogr. Relat. Technol. PD DEC 14 PY 2015 VL 38 IS 20 BP 1815 EP 1819 DI 10.1080/10826076.2015.1113546 PG 5 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CY1ZR UT WOS:000366208300007 ER PT J AU Thessen, AE Bunker, DE Buttigieg, PL Cooper, LD Dahdul, WM Domisch, S Franz, NM Jaiswal, P Lawrence-Dill, CJ Midford, PE Mungall, CJ Ramirez, MJ Specht, CD Vogt, L Vos, RA Walls, RL White, JW Zhang, GY Deans, AR Huala, E Lewis, SE Mabee, PM AF Thessen, Anne E. Bunker, Daniel E. Buttigieg, Pier Luigi Cooper, Laurel D. Dahdul, Wasila M. Domisch, Sami Franz, Nico M. Jaiswal, Pankaj Lawrence-Dill, Carolyn J. Midford, Peter E. Mungall, Christopher J. Ramirez, Martin J. Specht, Chelsea D. Vogt, Lars Vos, Rutger Aldo Walls, Ramona L. White, Jeffrey W. Zhang, Guanyang Deans, Andrew R. Huala, Eva Lewis, Suzanna E. Mabee, Paula M. TI Emerging semantics to link phenotype and environment SO PEERJ LA English DT Article DE Phenotype; Environment; Ontology; Semantic web; Biodiversity; Data integration ID PHIDIPPUS-CLARUS; SORGHUM-BICOLOR; JUMPING SPIDER; ONTOLOGY; BIODIVERSITY; INFORMATION; BIOLOGY; ANATOMY; UNIFICATION; VERTEBRATE AB Understanding the interplay between environmental conditions and phenotypes is a fundamental goal of biology. Unfortunately, data that include observations on phenotype and environment are highly heterogeneous and thus difficult to find and integrate. One approach that is likely to improve the status quo involves the use of ontologies to standardize and link data about phenotypes and environments. Specifying and linking data through ontologies will allow researchers to increase the scope and flexibility of large-scale analyses aided by modern computing methods. Investments in this area would advance diverse fields such as ecology, phylogenetics, and conservation biology. While several biological ontologies are well-developed, using them to link phenotypes and environments is rare because of gaps in ontological coverage and limits to interoperability among ontologies and disciplines. In this manuscript, we present (1) use cases from diverse disciplines to illustrate questions that could be answered more efficiently using a robust linkage between phenotypes and environments, (2) two proof-of-concept analyses that show the value of linking phenotypes to environments in fishes and amphibians, and (3) two proposed example data models for linking phenotypes and environments using the extensible observation ontology (OBOE) and the Biological Collections Ontology (BCO); these provide a starting point for the development of a data model linking phenotypes and environments. C1 [Thessen, Anne E.] Ronin Inst Independent Scholarship, Monclair, NJ 07043 USA. [Thessen, Anne E.] Data Detektiv, Waltham, MA USA. [Bunker, Daniel E.] New Jersey Inst Technol, Dept Biol Sci, Newark, NJ 07102 USA. [Buttigieg, Pier Luigi] Helmholtz Zentrum Polar & Meeresforsch, Alfred Wegener Inst, HGF MPG Grp Deep Sea Ecol & Technol, Bremerhaven, Germany. [Cooper, Laurel D.; Jaiswal, Pankaj] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Dahdul, Wasila M.; Mabee, Paula M.] Univ S Dakota, Dept Biol, Vermillion, SD 57069 USA. [Domisch, Sami] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT USA. [Franz, Nico M.; Zhang, Guanyang] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Lawrence-Dill, Carolyn J.] Iowa State Univ, Dept Genet, Ames, IA USA. [Lawrence-Dill, Carolyn J.] Iowa State Univ, Dept Dev & Cell Biol, Ames, IA USA. [Lawrence-Dill, Carolyn J.] Iowa State Univ, Dept Agron, Ames, IA USA. [Mungall, Christopher J.; Lewis, Suzanna E.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Genom Div, Berkeley, CA 94720 USA. [Ramirez, Martin J.] Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Natr, Div Arachnol, RA-1033 Buenos Aires, DF, Argentina. [Specht, Chelsea D.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Specht, Chelsea D.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Vogt, Lars] Univ Bonn, Inst Evolutionsbiol & Okol, Bonn, Germany. [Vos, Rutger Aldo] Nat Biodivers Ctr, Leiden, Netherlands. [Walls, Ramona L.] Univ Arizona, iPlant Collaborat, Tucson, AZ USA. [White, Jeffrey W.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Deans, Andrew R.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Huala, Eva] Phoenix Bioinformat, Redwood City, CA USA. RP Thessen, AE (reprint author), Ronin Inst Independent Scholarship, Monclair, NJ 07043 USA. EM annethessen@gmail.com RI Jaiswal, Pankaj/H-7599-2016; OI Jaiswal, Pankaj/0000-0002-1005-8383; Thessen, Anne/0000-0002-2908-3327; Lewis, Suzanna/0000-0002-8343-612X; Dahdul, Wasila/0000-0003-3162-7490; Buttigieg, Pier Luigi/0000-0002-4366-3088 FU US National Science Foundation [DEB-0956049, NSF IOS:0822201, IOS:1127112, IOS:1340112, DEB 1208666]; Micro B3 project - European Union [287589]; German Research Foundation DFG [DO 1880/1-1]; iPlant collaborative as part of the National Science Foundation Award [DBI-0735191, DBI-1265383]; Office of Science, Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231]; Phenoscape project (NSF grants) [DBI-1062404, DBI-1062542]; [R24OD011883] FX The scientific meeting from which this review arose was organized by the Phenotype Research Coordination Network, which is funded by the US National Science Foundation, grant number DEB-0956049. PLB's work on this project is supported through the Micro B3 project, funded by the European Union's Seventh Framework Programme (Joint Call OCEAN.2011-2: marine microbial diversity-new insights into marine ecosystems functioning and its biotechnological potential) under the grant agreement no 287589. SD received funding from the German Research Foundation DFG (grant DO 1880/1-1). PJ and LDC received funding from the US National Science Foundation (NSF IOS:0822201, IOS:1127112, IOS:1340112). CDS received funding from the US National Science Foundation grant number DEB 1208666. RLW was supported by the iPlant collaborative as part of the National Science Foundation Award Numbers DBI-0735191 and DBI-1265383. CJM and SEL were supported by R24OD011883 and by the Director, Office of Science, Office of Basic Energy Sciences, of the US Department of Energy under Contract No. DE-AC02-05CH11231. PMM and WMD were supported through by Phenoscape project (NSF grants DBI-1062404 and DBI-1062542). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 118 TC 2 Z9 2 U1 5 U2 16 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD DEC 14 PY 2015 VL 3 AR UNSP e1470 DI 10.7717/peerj.1470 PG 39 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CY4XY UT WOS:000366413400001 PM 26713234 ER PT J AU Wang, HF Fox, CS Troy, LM Mckeown, NM Jacques, PF AF Wang, Huifen Fox, Caroline S. Troy, Lisa M. Mckeown, Nicola M. Jacques, Paul F. TI Longitudinal association of dairy consumption with the changes in blood pressure and the risk of incident hypertension: the Framingham Heart Study SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Dairy intake; Blood pressure; Hypertension; Milk products; Yoghurt ID FOOD FREQUENCY QUESTIONNAIRE; JOINT NATIONAL COMMITTEE; MILK-FAT; ARTERIAL STIFFNESS; DIETARY PATTERNS; 7TH REPORT; WOMEN; MEN; HEALTH; REPRODUCIBILITY AB We aimed to examine the longitudinal association of dairy consumption with the changes in blood pressure (BP) and the risk of incident hypertension (HTN) among adults. This study included 2636 Framingham Heart Study Offspring Cohort members who participated in the 5th through 8th examinations (1991-2008) and were free of HTN at their first examination during the follow-up. Data collected at each examination included dietary intake (by a validated FFQ), BP (following standardised procedures) and anti-hypertensive medication use (by physician-elicited self-report). HTN was defined as systolic BP (SBP)140 mmHg, or diastolic BP (DBP)90 mmHg or anti-hypertensive medication use. We used repeated-measure and discrete-time hazard regressions to examine the associations of dairy consumption with the annualised BP change (n 2075) and incident HTN (n 2340; cases=1026), respectively. Covariates included demographic, lifestyle, overall diet quality, metabolic factors and medication use. Greater intakes of total dairy foods, total low-fat/fat-free dairy foods, low-fat/skimmed milk and yoghurt were associated with smaller annualised increments in SBP and a lower risk of projected HTN incidence. However, with the exception of total dairy foods and yoghurt, these inverse associations with HTN risk were attenuated as the follow-up time increased. For yoghurt, each additional serving was associated with 6 (95 % CI 1, 10) % reduced risk of incident HTN. Total dairy and total low-fat/fat-free dairy intakes were found to be inversely related to changes in DBP. Dairy consumption, as part of a nutritious and energy-balanced diet pattern, may benefit BP control and prevent or delay the onset of HTN. C1 [Wang, Huifen; Mckeown, Nicola M.; Jacques, Paul F.] Tufts Univ, Jean Mayer US Dept Agr USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fox, Caroline S.] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA. [Troy, Lisa M.] Univ Massachusetts, Chenoweth Lab, Dept Nutr, Amherst, MA 01003 USA. RP Jacques, PF (reprint author), Tufts Univ, Jean Mayer US Dept Agr USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM Paul.jacques@tufts.edu FU NHLBI [NO1-HC-25195]; US Department of Agriculture [58-1950-0-104]; Dannon Company Inc.; General Mills Bell Institute of Health and Nutrition FX This work was supported by NHLBI contract no. NO1-HC-25195, US Department of Agriculture Agreement 58-1950-0-104 and research grants from The Dannon Company Inc. and General Mills Bell Institute of Health and Nutrition. Any opinions, findings, conclusions or recommendations expressed in this paper are those of the authors and do not necessarily represent those of the NHLBI, the National Institutes of Health, the US Department of Health and Human Services or the US Department of Agriculture. The Dannon Company Inc. and General Mills Bell Institute of Health and Nutrition had no role in the design, analysis or writing of this article. NR 51 TC 7 Z9 7 U1 3 U2 18 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD DEC 14 PY 2015 VL 114 IS 11 BP 1887 EP 1899 DI 10.1017/S0007114515003578 PG 13 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CW7HC UT WOS:000365167800015 PM 26395861 ER PT J AU Ma, YJ Sun, XH Xu, XY Zhao, Y Pan, YJ Hwang, CA Wu, VCH AF Ma, Yue-jiao Sun, Xiao-hong Xu, Xiao-yan Zhao, Yong Pan, Ying-jie Hwang, Cheng-An Wu, Vivian C. H. TI Investigation of Reference Genes in Vibrio parahaemolyticus for Gene Expression Analysis Using Quantitative RT-PCR SO PLOS ONE LA English DT Article ID REVERSE TRANSCRIPTION-PCR; REAL-TIME PCR; HOUSEKEEPING GENES; TEMPERATURE REGULATION; VIRULENCE; QUANTIFICATION; NORMALIZATION; SELECTION; CONTRIBUTES; INTEGRITY AB Vibrio parahaemolyticus is a significant human pathogen capable of causing foodborne gastroenteritis associated with the consumption of contaminated raw or undercooked seafood. Quantitative RT-PCR (qRT-PCR) is a useful tool for studying gene expression in V. parahaemolyticus to characterize its virulence factors and understand the effect of environmental conditions on its pathogenicity. However, there is not a stable gene in V. parahaemolyticus that has been identified for use as a reference gene for qRT-PCR. This study evaluated the stability of 6 reference genes (16S rRNA, recA, rpoS, pvsA, pvuA, and gapdh) in 5 V. parahaemolyticus strains (O3:K6-clinical strain-tdh(+), ATCC33846-tdh(+), ATCC33847-tdh(+), ATCC17802-trh(+), and F13-environmental strain-tdh(+)) cultured at 4 different temperatures (15, 25, 37 and 42 degrees C). Stability values were calculated using GeNorm, NormFinder, BestKeeper, and Delta CT algorithms. The results indicated that recA was the most stably expressed gene in the V. parahaemolyticus strains cultured at different temperatures. This study examined multiple V. parahaemolyticus strains and growth temperatures, hence the finding provided stronger evidence that recA can be used as a reference gene for gene expression studies in V. parahaemolyticus. C1 [Ma, Yue-jiao; Sun, Xiao-hong; Zhao, Yong; Pan, Ying-jie] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China. [Ma, Yue-jiao; Sun, Xiao-hong; Zhao, Yong; Pan, Ying-jie] Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Shanghai 201306, Peoples R China. [Xu, Xiao-yan] Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai, Peoples R China. [Hwang, Cheng-An] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Wu, Vivian C. H.] Univ Maine, Pathogen Microbiol Lab, Sch Food & Agr, Orono, ME 04469 USA. [Wu, Vivian C. H.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Sun, XH (reprint author), Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai, Peoples R China. EM xhsun@shou.edu.cn; Vivian.Wu@ARS.USDA.GOV FU Shanghai Ocean University Doctoral Research Foundation; Key Project of Shanghai Agriculture Prosperity through Science and Technology [4-5]; Hatch Grant from the USDA National Institute of Food and Agriculture [ME08562-10] FX This work was supported by grants from Shanghai Ocean University Doctoral Research Foundation, Key Project of Shanghai Agriculture Prosperity through Science and Technology (2015, 4-5). This work is based upon research supported in part by Hatch Grant number ME08562-10 from the USDA National Institute of Food and Agriculture. NR 35 TC 2 Z9 2 U1 7 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 11 PY 2015 VL 10 IS 12 AR e0144362 DI 10.1371/journal.pone.0144362 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CZ1ZA UT WOS:000366903600038 PM 26659406 ER PT J AU Zeng, FF Zhao, ZF Yan, MJ Zhou, W Zhang, Z Zhang, AJ Lu, ZX Wang, MQ AF Zeng, Fang-Fang Zhao, Zhen-Fei Yan, Miao-Jun Zhou, Wen Zhang, Zan Zhang, Aijun Lu, Zhong-Xian Wang, Man-Qun TI Identification and Comparative Expression Profiles of Chemoreception Genes Revealed from Major Chemoreception Organs of the Rice Leaf Folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) SO PLOS ONE LA English DT Article ID ODORANT-BINDING PROTEINS; IONOTROPIC GLUTAMATE RECEPTORS; MOTH SPODOPTERA-LITTORALIS; SEX-PHEROMONE RECEPTOR; SILKMOTH BOMBYX-MORI; CHEMOSENSORY PROTEINS; FUNCTIONAL-CHARACTERIZATION; APIS-MELLIFERA; DROSOPHILA-MELANOGASTER; MEMBRANE-PROTEINS AB To better understand the olfactory mechanisms in the rice leaf folder, Cnaphalocrocis medinalis (Guene), a serious pest of rice in Asia, we established six partial transcriptomes from antennae, protarsus, and reproductive organs of male and female adults. A total of 102 transcripts were identified, including 29 odorant receptors (ORs), 15 ionotropic receptors (IRs), 30 odorant-binding proteins (OBPs), 26 chemosensory proteins (CSPs), and 2 sensory neuron membrane proteins (SNMPs). The expression patterns of these genes were calculated by fragments per kilobase of exon per million fragments mapped (FPKM) and validated by real-time quantitative PCR (RT-qPCR). Some transcripts were exclusively expressed in specific organs, such as female protarsus, whereas others were universally expressed, this varied expression profile may provide insights into the specific functions mediated by chemoreception proteins in insects. To the best of our knowledge, among the 102 identified transcripts, 81 are novel and have never been reported before. In addition, it also is the first time that ORs and IRs are identified in C. medinalis. Our findings significantly enhance the currently limited understanding olfactory mechanisms of the olfactory mechanisms underlying the chemoreception system in C. medinalis. C1 [Zeng, Fang-Fang; Zhao, Zhen-Fei; Yan, Miao-Jun; Zhou, Wen; Zhang, Zan; Wang, Man-Qun] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Zhang, Aijun] ARS, Invas Insect Biocontrol & Behav Lab, BARC West, USDA, Beltsville, MD 20705 USA. [Lu, Zhong-Xian] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National High Technology Research and Development Program of China (863 Program) [2014AA10A605]; China Agriculture Research System [CARS-01-17]; Fundamental Research Funds for the Central Universities [2013PY046] FX This study was supported and funded by the National High Technology Research and Development Program of China (863 Program) (No. 2014AA10A605), the China Agriculture Research System (Grant No. CARS-01-17) and the Fundamental Research Funds for the Central Universities (2013PY046). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 63 TC 1 Z9 1 U1 7 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 11 PY 2015 VL 10 IS 12 AR e0144267 DI 10.1371/journal.pone.0144267 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CZ1ZA UT WOS:000366903600033 PM 26657286 ER PT J AU Clayton, KA Gall, CA Mason, KL Scoles, GA Brayton, KA AF Clayton, Katie A. Gall, Cory A. Mason, Katheen L. Scoles, Glen A. Brayton, Kelly A. TI The characterization and manipulation of the bacterial microbiome of the Rocky Mountain wood tick, Dermacentor andersoni SO PARASITES & VECTORS LA English DT Article DE Ticks; Microbiome; Endosymbiont ID FRANCISELLA-LIKE ENDOSYMBIONTS; AMBLYOMMA-AMERICANUM; WESTERN MONTANA; RICKETTSIAE; ARTHROPOD; DIVERSITY; DISEASES; GENES; HOST AB Background: In North America, ticks are the most economically impactful vectors of human and animal pathogens. The Rocky Mountain wood tick, Dermacentor andersoni (Acari: Ixodidae), transmits Rickettsia rickettsii and Anaplasma marginale to humans and cattle, respectively. In recent years, studies have shown that symbiotic organisms are involved in a number of biochemical and physiological functions. Characterizing the bacterial microbiome of D. andersoni is a pivotal step towards understanding symbiont-host interactions. Findings: In this study, we have shown by high-throughput sequence analysis that the composition of endosymbionts in the midgut and salivary glands in adult ticks is dynamic over three generations. Four Proteobacteria genera, Rickettsia, Francisella, Arsenophonus, and Acinetobacter, were identified as predominant symbionts in these two tissues. Exposure to therapeutic doses of the broad-spectrum antibiotic, oxytetracycline, affected both proportions of predominant genera and significantly reduced reproductive fitness. Additionally, Acinetobacter, a free-living ubiquitous microbe, invaded the bacterial microbiome at different proportions based on antibiotic treatment status suggesting that microbiome composition may have a role in susceptibility to environmental contaminants. Conclusions: This study characterized the bacterial microbiome in D. andersoni and determined the generational variability within this tick. Furthermore, this study confirmed that microbiome manipulation is associated with tick fitness and may be a potential method for biocontrol. C1 [Clayton, Katie A.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Gall, Cory A.; Brayton, Kelly A.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Mason, Katheen L.; Scoles, Glen A.] Washington State Univ, ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Brayton, KA (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM kbrayton@vetmed.wsu.edu OI Gall, Cory/0000-0001-6973-3649 FU NIH [AI4405]; NIH/NIGMS [T32 GM008336]; NSF ADVANCE transitions grant; CVM intramural funds; [USDA-ARS-CRIS5348-32000-033-00D] FX We would like to thank Ralph Horn, James Allison, Xiaoya Cheng, and Sara Davis for technical assistance. This work was supported by NIH AI4405, USDA-ARS-CRIS5348-32000-033-00D, NIH/NIGMS-funded predoctoral fellowship (T32 GM008336), an NSF ADVANCE transitions grant, and CVM intramural funds. NR 25 TC 2 Z9 2 U1 4 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD DEC 10 PY 2015 VL 8 AR 632 DI 10.1186/s13071-015-1245-z PG 5 WC Parasitology SC Parasitology GA CY0OX UT WOS:000366106500002 PM 26653035 ER PT J AU Muthukrishnan, R West, NM Davis, AS Jordan, NR Forester, JD AF Muthukrishnan, Ranjan West, Natalie M. Davis, Adam S. Jordan, Nicholas R. Forester, James D. TI Evaluating the role of landscape in the spread of invasive species: The case of the biomass crop Miscanthus x giganteus SO ECOLOGICAL MODELLING LA English DT Article DE Invasive species; Biofuel; Landscape; Integro-difference equation model; Fragmentation ID LONG-DISTANCE DISPERSAL; ECOLOGICAL EXPLANATION; FRAGMENTED LANDSCAPES; PLANT INVASIONS; MANAGEMENT; BIOFUELS; MODELS; CONSEQUENCES; RESISTANCE; SECURITY AB The introduction and spread of potentially invasive species present profound ecological challenges with major consequences for natural and cultivated ecosystems. The spread of invasive species is driven by both invader traits and the landscapes they are colonizing and there is a pressing need for objective and quantitative methods that integrate landscape details into predictions of biological invasions. Here we develop a new spatially explicit integro-difference equation model to predict the spread of invasive species over real landscapes. We use this model to evaluate the spread of a potentially invasive biomass crop, namely a fertile variety of Miscanthus giganteus, as a case study of how such models can be used to aid decision making when managing agricultural landscapes. We show that M. giganteus has the ability to invade large landscapes, but the rate and extent of that spread is strongly dependent on the landscape, including composition, spatial structure and the presence of dispersal corridors. Lastly, we discuss the potential application and value of spatial models in risk analysis and management of novel agricultural production systems that include potentially invasive crops. (C) 2015 Elsevier B.V. All rights reserved. C1 [Muthukrishnan, Ranjan; Forester, James D.] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA. [West, Natalie M.; Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Washington, DC 20250 USA. [Jordan, Nicholas R.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. RP Muthukrishnan, R (reprint author), Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, 135 Skok Hall,2003 Upper Buford Cr, St Paul, MN 55108 USA. EM mrunj@umn.edu; nmwest@illinois.edu; asdavis1@illinois.edu; jorda020@umn.edu; jdforest@umn.edu FU USDA-NIFA [2012-67013] FX We are grateful for resources from the University of Minnesota Supercomputing Institute. We also appreciate the assistance of Jeff Cardille with the METALAND database and the comments of two anonymous reviewers, which improved the manuscript. This work was funded by USDA-NIFA grant #2012-67013 to NRJ, ASD and JDF. NR 57 TC 2 Z9 2 U1 8 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD DEC 10 PY 2015 VL 317 BP 6 EP 15 DI 10.1016/j.ecolmodel.2015.08.022 PG 10 WC Ecology SC Environmental Sciences & Ecology GA CW5UZ UT WOS:000365063400002 ER PT J AU Shirk, PD Perera, OP Shelby, KS Furlong, RB LoVullo, ED Popham, HJR AF Shirk, Paul D. Perera, Omaththage P. Shelby, Kent S. Furlong, Richard B. LoVullo, Eric D. Popham, Holly J. R. TI Unique synteny and alternate splicing of the chitin synthases in closely related heliothine moths SO GENE LA English DT Article DE Genetic linkage; Corn earworm; Cotton bollworm; Tobacco budworm; Cuticle; Peritrophic membrane ID ECDYSONE RESPONSE ELEMENTS; INSECT OSTRINIA-FURNACALIS; RED FLOUR BEETLE; PERITROPHIC MATRIX; TRIBOLIUM-CASTANEUM; GENE-EXPRESSION; MANDUCA-SEXTA; PHYSIOLOGICAL SIGNIFICANCE; DROSOPHILA-MELANOGASTER; EMBRYONIC-DEVELOPMENT AB Chitin is an extracellular biopolymer that contributes to the cuticular structural matrix in arthropods. As a consequence of its rigid structure, the chitinous cuticle must be shed and replaced to accommodate growth. Two chitin synthase genes that encode for chitin synthase A (ChSA), which produces cuticular exoskeleton, and chitin synthase B (ChSB), which produces peritrophic membrane, were characterized in the genomes of two heliothine moths: the corn earworm/cotton bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) and the cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). In both moths, the two genes were arranged in tandem with the same orientation on the same strand with ChSB located 5' of ChSA. Sequence comparisons showed that the coding sequences were highly conserved with homologues from other species but that the tandem juxtaposed genomic arrangement of the two genes was unique in these insects. The mechanism that has led to this arrangement is unclear but is most likely a recent recombinational event. Transcript mapping of HzChSB and HzChSA in H. zea demonstrated that both transcripts were differentially spliced in various tissues and larval stages. The identification of the HzChSB-E12b alternate spliced transcript is the first report of alternate splicing for the ChSB group. The importance of this splice form is not clear because the protein produced would lack any enzymatic activity but retain the membrane insertion motifs. As for other insects, these genes provide an important target for potential control through RNAi but also provide a subject for broad scale genomic recombinational events. Published by Elsevier B.V. C1 [Shirk, Paul D.; Furlong, Richard B.; LoVullo, Eric D.] USDA ARS, CMAVE, Gainesville, FL 32608 USA. [Perera, Omaththage P.] USDA ARS, SIMRU, Stoneville, MI 38776 USA. [Shelby, Kent S.; Popham, Holly J. R.] USDA ARS, BCIRL, Columbia, MO 65203 USA. RP Shirk, PD (reprint author), USDA ARS, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM Paul.Shirk@ars.usda.gov; op.perera@ars.usda.gov; kent.shelby@ars.usda.gov; richard.furlong@ars.usda.gov; eric.lovullo@ars.usda.gov; hpopham@agbitech.com NR 66 TC 0 Z9 0 U1 3 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD DEC 10 PY 2015 VL 574 IS 1 BP 121 EP 139 DI 10.1016/j.gene.2015.08.001 PG 19 WC Genetics & Heredity SC Genetics & Heredity GA CV4OR UT WOS:000364246900017 PM 26253161 ER PT J AU do Nascimento, CAC Pagliari, PH Schmitt, D He, ZQ Waldrip, H AF do Nascimento, Carlos A. C. Pagliari, Paulo H. Schmitt, Djalma He, Zhongqi Waldrip, Heidi TI Phosphorus Concentrations in Sequentially Fractionated Soil Samples as Affected by Digestion Methods SO SCIENTIFIC REPORTS LA English DT Article ID ATOMIC EMISSION-SPECTROMETRY; TOTAL DISSOLVED PHOSPHORUS; ORGANIC PHOSPHORUS; ENZYMATIC-HYDROLYSIS; HEDLEY FRACTIONATION; POULTRY MANURE; ANIMAL MANURE; SWINE MANURE; DYNAMICS; FORMS AB Sequential fractionation has helped improving our understanding of the lability and bioavailability of P in soil. Nevertheless, there have been no reports on how manipulation of the different fractions prior to analyses affects the total P (TP) concentrations measured. This study investigated the effects of sample digestion, filtration, and acidification on the TP concentrations determined by ICP-OES in 20 soil samples. Total P in extracts were either determined without digestion by ICP-OES, or ICP-OES following block digestion, or autoclave digestion. The effects of sample filtration, and acidification on undigested alkaline extracts prior to ICP-OES were also evaluated. Results showed that, TP concentrations were greatest in the block-digested extracts, though the variability introduced by the block-digestion was the highest. Acidification of NaHCO3 extracts resulted in lower TP concentrations, while acidification of NaOH randomly increased or decreased TP concentrations. The precision observed with ICP-OES of undigested extracts suggests this should be the preferred method for TP determination in sequentially extracted samples. Thus, observations reported in this work would be helpful in appropriate sample handling for P determination, thereby improving the precision of P determination. The results are also useful for literature data comparison and discussion when there are differences in sample treatments. C1 [do Nascimento, Carlos A. C.] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, BR-13418900 Piracicaba, SP, Brazil. [Pagliari, Paulo H.] Univ Minnesota, Southwest Res & Outreach Ctr, Dept Soil Water & Climate, Minneapolis, MN 56152 USA. [Schmitt, Djalma] Santa Catarina State Univ, Lages, SC, Brazil. [He, Zhongqi] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Waldrip, Heidi] ARS, USDA, Conservat & Prod Res Lab, Bushland, TX 79012 USA. RP Pagliari, PH (reprint author), Univ Minnesota, Southwest Res & Outreach Ctr, Dept Soil Water & Climate, 23669 130th St Lamberton, Minneapolis, MN 56152 USA. EM pagli005@umn.edu RI Schmitt, Djalma/E-6471-2013; Costa do Nascimento, Carlos Antonio/K-8598-2012 OI Schmitt, Djalma/0000-0001-9665-9704; NR 43 TC 5 Z9 5 U1 6 U2 26 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 9 PY 2015 VL 5 AR 17967 DI 10.1038/srep17967 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX9MY UT WOS:000366030900001 PM 26647644 ER PT J AU Lopez, JMP Johnson, PJ Gagne, RJ Boe, A AF Lopez, J. Manuel Perilla Johnson, Paul J. Gagne, Raymond J. Boe, Arvid TI A new species of Stenodiplosis (Diptera: Cecidomyiidae) on Spartina grasses (Poaceae) with notes on its biology and its parasitoid Tetrastichus bromi (Hymenoptera: Eulophidae) SO ZOOTAXA LA English DT Article DE taxonomy; prairie cordgrass; seed predator; introduced species; parasitoid guild ID PRAIRIE CORDGRASS; BIG BLUESTEM; SEED MIDGE; GENOTYPES; SORGHUM; SET AB Stenodiplosis spartinae Gagne new species (Diptera: Cecidomyiidae) is described from eastern South Dakota and coastal North Carolina, and compared with other American congeners. The known host plants are Spartina alterniflora and S. pectinata. The larva is a seed predator of the ovule and immature caryopsis of the host plant. Adult activity is from the early emergence of the host inflorescence through anthesis. Oviposition occurs in the floret with eggs laid under the edges of the palea and lemma. The larva apparently overwinters in dehisced spikelets in the soil among rhizomes of S. pectinata, with pupation in late spring. Laboratory emergence and field activity of the adults suggest a potential second or third generation developing on late emerging inflorescences. Larval feeding does not induce external color or shape changes in the spikelet. Apparently all three instars are ectoparasitized by Tetrastichus bromi Kostyukov (Hymenoptera: Eulophidae) that was probably introduced to North America in the late 1800's and is inculcated into parasitoid guilds of several Stenodiplosis species. Resource partitioning appears to occur between the gall midge and early instars of Aethes spartinana Barnes and McDunnough (Lepidoptera: Tortricidae) that feed on maturing caryopses. The feeding of this gall midge and the moth probably account for most of the reduced seed production in both natural and agronomic populations of S. pectinata. C1 [Lopez, J. Manuel Perilla; Johnson, Paul J.; Boe, Arvid] S Dakota State Univ, Insect Biodivers Lab, Brookings, SD 57007 USA. [Gagne, Raymond J.] ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst, Washington, DC 20013 USA. RP Gagne, RJ (reprint author), ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. EM juan.perilla@sdstate.edu; paul.johnson@sdstate.edu; raymond.gagne@ars.usda.gov; arvid.boe@sdstate.edu FU Oak Lake Research Fund; North Central Regional Sun Grant Initiative at South Dakota State University through grant from the U.S. Department of Energy Bioenergy Technologies Office [DE-FG36-08GO88073]; North Central Regional Sun Grant Initiative at South Dakota State University through grant from the U.S. Department of Agriculture [2010-38502-21861] FX We are grateful to Bradley J. Sinclair, Canadian National Collection of Insects and Ottawa Plant Laboratory, Ottawa, Ontario, for a loan of Stenodiplosis phragmicola; Mike Althaus, Silver Spring, MD, for arrangement and labeling of Figures 1-13; and Michael Gates, USDA-ARS-SEL, National Museum of Natural History, and Zoya Yefremova, Tel Aviv University for taxonomic assistance with Aprostocetus. Sally Gagne is thanked for her assistance in proofreading the penultimate manuscript draft. Netta Dorchin, Tel Aviv University, Israel, and Junichi Yukawa, Kyushu University, Japan, provided useful comments on a previous version of this manuscript. Fieldwork was done at the South Dakota State University Oak Lake Field Station, the Aurora Crop Improvement Research Farm and the South Dakota Agricultural Experiment Station Felt Family Farm, all in Brookings County, South Dakota. The research leading to this report was supported by the Oak Lake Research Fund, and the North Central Regional Sun Grant Initiative at South Dakota State University through grants from the U.S. Department of Energy Bioenergy Technologies Office under award number DE-FG36-08GO88073, and the U.S. Department of Agriculture under award number 2010-38502-21861. NR 42 TC 1 Z9 1 U1 2 U2 8 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD DEC 9 PY 2015 VL 4057 IS 1 BP 115 EP 124 PG 10 WC Zoology SC Zoology GA CY1YU UT WOS:000366205300007 PM 26701469 ER PT J AU Chopra, R Burow, G Hayes, C Emendack, Y Xin, ZG Burke, J AF Chopra, Ratan Burow, Gloria Hayes, Chad Emendack, Yves Xin, Zhanguo Burke, John TI Transcriptome profiling and validation of gene based single nucleotide polymorphisms (SNPs) in sorghum genotypes with contrasting responses to cold stress SO BMC GENOMICS LA English DT Article DE Cold stress; Sorghum; Transcriptome profiling; RNAseq; Single nucleotide polymorphism; Gene based variants ID HEAT-SHOCK PROTEINS; RNA-SEQ; TOLERANCE; EXPRESSION; RICE; DROUGHT; OVEREXPRESSION; IDENTIFICATION; ARABIDOPSIS; BICOLOR AB Background: Sorghum is a versatile cereal crop, with excellent heat and drought tolerance. However, it is susceptible to early-season cold stress (12-15 degrees C) which limits stand-establishment and seedling growth. To gain further insights on the molecular mechanism of cold tolerance in sorghum we performed transcriptome profiling between known cold sensitive and tolerant sorghum lines using RNA sequencing technology under control and cold stress treatments. Results: Here we report on the identification of differentially expressed genes (DEGs) between contrasting sorghum genotypes, HongkeZi (cold tolerant) and BTx623 (cold sensitive) under cool and control temperatures using RNAseq approach to elucidate the molecular basis of sorghum response to cold stress. Furthermore, we validated bi-allelic variants in the form of single nucleotide polymorphism (SNPs) between the cold susceptible and tolerant lines of sorghum. An analysis of transcriptome profile showed that in response to cold, a total of 1910 DEGs were detected under cold and control temperatures in both genotypes. We identified a subset of genes under cold stress for downstream analysis, including transcription factors that exhibit differential abundance between the sensitive and tolerant genotypes. We identified transcription factors including Dehydration-responsive element-binding factors, C-repeat binding factors, and Ethylene responsive transcription factors as significantly upregulated during cold stress in cold tolerant HKZ. Additionally, specific genes such as plant cytochromes, glutathione s-transferases, and heat shock proteins were found differentially regulated under cold stress between cold tolerant and susceptible genotype of sorghum. A total of 41,603 SNP were identified between the cold sensitive and tolerant genotypes with minimum read of four. Approximately 89 % of the 114 SNP sites selected for evaluation were validated using endpoint genotyping technology. Conclusion: A new strategy which involved an integrated analysis of differential gene expression and identification of bi-allelic single nucleotide polymorphism (SNP) was conducted to determine and analyze differentially expressed genes and variation involved in cold stress response of sorghum. The results gathered provide an insight into the complex mechanisms associated with cold response in sorghum, which involve an array of transcription factors and genes which were previously related to abiotic stress response. This study also offers resource for gene based SNP that can be applied towards targeted genomic studies of cold tolerance in sorghum and other cereal crops. C1 [Chopra, Ratan; Burow, Gloria; Hayes, Chad; Emendack, Yves; Xin, Zhanguo; Burke, John] ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, USDA, Lubbock, TX 79415 USA. RP Burow, G (reprint author), ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, USDA, Lubbock, TX 79415 USA. EM Gloria.burow@ars.usda.gov OI CHOPRA, RATAN/0000-0003-2088-3341 FU United Sorghum Checkoff program through the project: "Genetic Enhancement of Sorghums" FX We would like to thank United Sorghum Checkoff program for funding through the project: "Genetic Enhancement of Sorghums". We also acknowledge National Center for Genome Resources, Santa Fe, NM including Ms. Faye Schilkey and Andrew Farmer. The excellent technical support of Ms. Halee Huhges and Ms. Kayla Kersh are appreciated. NR 37 TC 3 Z9 3 U1 8 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 9 PY 2015 VL 16 AR 1040 DI 10.1186/s12864-015-2268-8 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CY0MI UT WOS:000366099800001 PM 26645959 ER PT J AU Yue, JY Liu, J Ban, RJ Tang, W Deng, L Fei, ZJ Liu, YS AF Yue, Junyang Liu, Jian Ban, Rongjun Tang, Wei Deng, Lin Fei, Zhangjun Liu, Yongsheng TI Kiwifruit Information Resource (KIR): a comparative platform for kiwifruit genomics SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID PROTEIN FAMILIES DATABASE; TRANSCRIPTION FACTORS; ACTINIDIA-CHINENSIS; CLASSIFICATION; SEQUENCE; NETWORK; ANNOTATION; SYSTEM; EVOLUTIONARY; PHOSPHATASES AB The Kiwifruit Information Resource (KIR) is dedicated to maintain and integrate comprehensive datasets on genomics, functional genomics and transcriptomics of kiwifruit (Actinidiaceae). KIR serves as a central access point for existing/new genomic and genetic data. KIR also provides researchers with a variety of visualization and analysis tools. Current developments include the updated genome structure of Actinidia chinensis cv. Hongyang and its newest genome annotation, putative transcripts, gene expression, physical markers of genetic traits as well as relevant publications based on the latest genome assembly. Nine thousand five hundred and forty-seven new transcripts are detected and 21 132 old transcripts are changed. At the present release, the next-generation transcriptome sequencing data has been incorporated into gene models and splice variants. Protein-protein interactions are also identified based on experimentally determined orthologous interactions. Furthermore, the experimental results reported in peer-reviewed literature are manually extracted and integrated within a well-developed query page. In total, 122 identifications are currently associated, including commonly used gene names and symbols. All KIR datasets are helpful to facilitate a broad range of kiwifruit research topics and freely available to the research community. C1 [Yue, Junyang; Liu, Jian; Tang, Wei; Liu, Yongsheng] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. [Ban, Rongjun] Univ Sci & Technol China, Sch Informat Sci & Technol, Hefei 230009, Peoples R China. [Deng, Lin] Hefei Univ Technol, Informat & Network Ctr, Hefei 230009, Peoples R China. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA ARS, Robert W Holley Ctr, Ithaca, NY 14853 USA. [Liu, Yongsheng] Sichuan Univ, Coll Life Sci, Minist Educ Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Peoples R China. [Liu, Yongsheng] Sichuan Univ, State Key Lab Hydraul & Mt River Engn, Chengdu 610064, Peoples R China. RP Liu, YS (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. EM liuyongsheng1122@hfut.edu.cn FU National Natural Science Foundation of China [31171179, 31471157, 31461143008]; National Science and Technology Key Project of China [2011CB100401]; National Science Fund for Distinguished Young Scholars [30825030]; Advanced Program of Doctoral Fund of Ministry of Education of China [20110181130009]; Government of Sichuan Province [2013NZ0014]; Government of Anhui Province [2012AKKG0739] FX This work was supported by funds from the National Natural Science Foundation of China (31171179, 31471157 and 31461143008); the National Science and Technology Key Project of China (2011CB100401); the National Science Fund for Distinguished Young Scholars (30825030); Advanced Program of Doctoral Fund of Ministry of Education of China (20110181130009); a Key Project from the Government of Sichuan Province (2013NZ0014); a Project from the Government of Anhui Province (2012AKKG0739). Funding for open access charge: the National Natural Science Foundation of China (31461143008). NR 44 TC 1 Z9 1 U1 3 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD DEC 9 PY 2015 AR bav113 DI 10.1093/database/bav113 PG 8 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA CX9TF UT WOS:000366047800001 ER PT J AU Steffan, SA Chikaraishi, Y Currie, CR Horn, H Gaines-Day, HR Pauli, JN Zalapa, JE Ohkouchi, N AF Steffan, Shawn A. Chikaraishi, Yoshito Currie, Cameron R. Horn, Heidi Gaines-Day, Hannah R. Pauli, Jonathan N. Zalapa, Juan E. Ohkouchi, Naohiko TI Microbes are trophic analogs of animals SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE compound specific; food chain; leaf-cutter ant; microbe; stable isotope ID NITROGEN ISOTOPIC COMPOSITION; FUNGUS-GROWING ANTS; AQUATIC FOOD-WEB; AMINO-ACIDS; DIET; BIODIVERSITY; ECOSYSTEM; BACTERIA; EARTH AB In most ecosystems, microbes are the dominant consumers, commandeering much of the heterotrophic biomass circulating through food webs. Characterizing functional diversity within the microbiome, therefore, is critical to understanding ecosystem functioning, particularly in an era of global biodiversity loss. Using isotopic fingerprinting, we investigated the trophic positions of a broad diversity of heterotrophic organisms. Specifically, we examined the naturally occurring stable isotopes of nitrogen (N-15: N-14) within amino acids extracted from proteobacteria, actinomycetes, ascomycetes, and basidiomycetes, as well as from vertebrate and invertebrate macrofauna (crustaceans, fish, insects, and mammals). Here, we report that patterns of intertrophic N-15-discrimination were remarkably similar among bacteria, fungi, and animals, which permitted unambiguous measurement of consumer trophic position, independent of phylogeny or ecosystem type. The observed similarities among bacterial, fungal, and animal consumers suggest that within a trophic hierarchy, microbiota are equivalent to, and can be interdigitated with, macrobiota. To further test the universality of this finding, we examined Neotropical fungus gardens, communities in which bacteria, fungi, and animals are entwined in an ancient, quadripartite symbiosis. We reveal that this symbiosis is a discrete four-level food chain, wherein bacteria function as the apex carnivores, animals and fungi are meso-consumers, and the sole herbivores are fungi. Together, our findings demonstrate that bacteria, fungi, and animals can be integrated within a food chain, effectively uniting the macro-and microbiome in food web ecology and facilitating greater inclusion of the microbiome in studies of functional diversity. C1 [Steffan, Shawn A.; Gaines-Day, Hannah R.] Univ Wisconsin, Dept Entomol, Madison, WI 53705 USA. [Steffan, Shawn A.; Zalapa, Juan E.] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA. [Chikaraishi, Yoshito; Ohkouchi, Naohiko] Japan Agcy Marine Earth Sci & Technol, Dept Biogeochem, Yokosuka, Kanagawa 2370061, Japan. [Currie, Cameron R.; Horn, Heidi] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Pauli, Jonathan N.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. RP Steffan, SA (reprint author), Univ Wisconsin, Dept Entomol, Madison, WI 53705 USA. EM steffan@entomology.wisc.edu FU University of Wisconsin Vilas Lifecycle Professorship; US Department of Agriculture-Agricultural Research Service [3655-21220-001]; Japan Agency for Marine-Earth Science and Technology FX We thank Janet van Zoeren, Rachel Arango, Sacha Horn, Lindsay Wells, Brian Hudelson, Christopher Watson, Merritt Singleton, and Drs. Patricia McManus, Bhadriraju Subramanyam, Tess Killpack, and Bill Karasov for assistance with animal and microbial cultures. Leaf-cutter ant photos appear courtesy of Don Parsons. Drs. Prarthana Dharampal, Peggy Ostrom, Stephen Carpenter, and Elissa Chasen provided helpful suggestions on earlier manuscript drafts. This work was supported by the University of Wisconsin Vilas Lifecycle Professorship (awarded to S.A.S.), the US Department of Agriculture-Agricultural Research Service (Current Research Information System 3655-21220-001, awarded to S.A.S. and J.E.Z.), and the Japan Agency for Marine-Earth Science and Technology. NR 34 TC 6 Z9 6 U1 23 U2 75 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD DEC 8 PY 2015 VL 112 IS 49 BP 15119 EP 15124 DI 10.1073/pnas.1508782112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX8YA UT WOS:000365989800044 PM 26598691 ER PT J AU Wang, L Zeng, L Chen, J AF Wang, Lei Zeng, Ling Chen, Jian TI Impact of imidacloprid on new queens of imported fire ants, Solenopsis invicta (Hymenoptera: Formicidae) SO SCIENTIFIC REPORTS LA English DT Article ID NEONICOTINOID PESTICIDES; INCIPIENT COLONIES; EGG-PRODUCTION; EXPOSURE; INSECTICIDES; HONEYBEES; FIPRONIL; INVERTEBRATES; MEMORY; ACARI AB Neonicotinoid insecticides are commonly used in managing pest insects, including the imported fire ant, Solenopsis invicta Buren. There is increasing evidence that neonicotinoid insecticides at sublethal concentrations have profound effects on social insects. However, the sublethal effect of neonicotinoids on S. invicta has never been investigated. In this study, the newly mated queens were fed with water containing 0.01 or 0.25 mu g/ml imidacloprid. Imidacloprid at both concentrations did not cause any increase in queen mortality during the founding stage; however, it significantly reduced queens' brood tending ability. In the 0.25 mu g/ml imidacloprid treatment, the time to larval emergence was significantly delayed and no pupae or adult workers were produced. This study provides clear evidence that imidacloprid at sublethal concentrations has a significant detrimental impact on S. invicta queens and the development of incipient colonies. C1 [Wang, Lei; Zeng, Ling] South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China. [Chen, Jian] ARS, Nat Biol Control Lab, Southeast Area, USDA, Stoneville, MS 38776 USA. RP Chen, J (reprint author), ARS, Nat Biol Control Lab, Southeast Area, USDA, 59 Lee Rd, Stoneville, MS 38776 USA. EM jian.chen@ars.usda.gov FU Hesheng Zhujiang Education Foundation FX We thank Dr. Walker A. Jones, USDA-ARS, Stoneville, MS and Dr. Beverly Wiltz, Delgado Community College, New Orleans, LA for critical reviews of the manuscript. We thank Mr. Leon Hicks for his technical assistance. This project is supported by the Hesheng Zhujiang Education Foundation. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 48 TC 2 Z9 2 U1 1 U2 26 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 8 PY 2015 VL 5 AR 17938 DI 10.1038/srep17938 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX8PF UT WOS:000365965300001 PM 26643971 ER PT J AU Zheljazkov, VD Gawde, A Cantrell, CL Astatkie, T Schlegel, V AF Zheljazkov, Valtcho D. Gawde, Archana Cantrell, Charles L. Astatkie, Tess Schlegel, Vicki TI Distillation Time as Tool for Improved Antimalarial Activity and Differential Oil Composition of Cumin Seed Oil SO PLOS ONE LA English DT Article ID ARTEMISIA-ANNUA L.; ANTIOXIDANT CAPACITY; YIELD; ASSAY AB A steam distillation extraction kinetics experiment was conducted to estimate essential oil yield, composition, antimalarial, and antioxidant capacity of cumin (Cuminum cyminum L.) seed (fruits). Furthermore, regression models were developed to predict essential oil yield and composition for a given duration of the steam distillation time (DT). Ten DT durations were tested in this study: 5, 7.5, 15, 30, 60, 120, 240, 360, 480, and 600 min. Oil yields increased with an increase in the DT. Maximum oil yield (content, 2.3 g/100 seed), was achieved at 480 min; longer DT did not increase oil yields. The concentrations of the major oil constituents alpha-pinene (0.14-0.5% concentration range), beta-pinene (3.7-10.3% range),gamma-cymene (5-7.3% range),gamma-terpinene (1.8-7.2% range), cumin aldehyde (50-66% range), alpha-terpinen-7-al (3.8-16% range), and beta-terpinen-7-al (12-20% range) varied as a function of the DT. The concentrations of alpha-pinene, beta-pinene, gamma-cymene, gamma-terpinene in the oil increased with the increase of the duration of the DT; alpha-pinene was highest in the oil obtained at 600 min DT, beta-pinene and alpha-terpinene reached maximum concentrations in the oil at 360 min DT; gamma-cymene reached a maximum in the oil at 60 min DT, cumin aldehyde was high in the oils obtained at 5-60 min DT, and low in the oils obtained at 240-600 min DT, gamma-terpinen-7-al reached maximum in the oils obtained at 480 or 600 min DT, whereas beta-terpinen-7-al reached a maximum concentration in the oil at 60 min DT. The yield of individual oil constituents (calculated from the oil yields and the concentration of a given compound at a particular DT) increased and reached a maximum at 480 or 600 min DT. The antimalarial activity of the cumin seed oil obtained during the 0-5 and at 5-7.5 min DT time-frames was twice higher than the antimalarial activity of the oils obtained at the other DT. This study opens the possibility for distinct marketing and utilization for these improved oils. The antioxidant capacity of the oil was highest in the oil obtained at 30 min DT and lowest in the oil from 360 min DT. The Michaelis-Menton and the Power nonlinear regression models developed in this study can be utilized to predict essential oil yield and composition of cumin seed at any given duration of DT and may also be useful to compare previous reports on cumin oil yield and composition. DT can be utilized to obtain cumin seed oil with improved antimalarial activity, improved antioxidant capacity, and with various compositions. C1 [Zheljazkov, Valtcho D.] Oregon State Univ, Columbia Basin Agr Res Ctr, Adams, OR 97810 USA. [Gawde, Archana; Cantrell, Charles L.] USDA, Nat Prod Utilizat Res Unit, Agr Res Serv, University, MS 38677 USA. [Astatkie, Tess] Dalhousie Univ, Fac Agr, Truro, NS B2N 5E3, Canada. [Schlegel, Vicki] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. RP Zheljazkov, VD (reprint author), Oregon State Univ, Columbia Basin Agr Res Ctr, 48037 Cty 788 Rd, Adams, OR 97810 USA. EM Valtcho.jeliazkov@oregonstate.edu RI Astatkie, Tess/J-7231-2012; OI Astatkie, Tess/0000-0002-9779-8789; Gawde, Archana/0000-0002-6495-3850 FU University of Wyoming Outreach School; Department of Plant Science FX Financial support was provided in part by the University of Wyoming Outreach School and the Department of Plant Science allocations to Valtcho D. Jeliazkov (V.D. Zheljazkov).; The authors thank Jeremiah Vardiman from the University of Wyoming Sheridan Research and Extension Center for their help with the laboratory work, the extraction and measurements of the essential oils, and Rochelle Koltiska for proofreading the manuscript. The authors also thank Solomon Green III for technical assistance at the National Center for Natural Products Research in Oxford, MS and USDA-ARS. Financial support was provided in part by the University of Wyoming Outreach School and the Department of Plant Science allocations to Valtcho D. Jeliazkov (V.D. Zheljazkov). NR 24 TC 0 Z9 0 U1 1 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 7 PY 2015 VL 10 IS 12 AR e0144120 DI 10.1371/journal.pone.0144120 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CZ1YS UT WOS:000366902700058 PM 26641276 ER PT J AU Yuan, XJ Yan, CH Wu, ZJ Ren, FH Zhang, H Baker, B Chen, JJ Kuang, HH AF Yuan, Xinjie Yan, Chenghuan Wu, Zhujun Ren, Feihong Zhang, Hui Baker, Barbara Chen, Jiongjiong Kuang, Hanhui TI Frequent Gain and Loss of Resistance against Tobacco Mosaic Virus in Nicotiana Species SO MOLECULAR PLANT LA English DT Letter ID EVOLUTION; FAMILY; GENES C1 [Yuan, Xinjie; Yan, Chenghuan; Wu, Zhujun; Ren, Feihong; Zhang, Hui; Chen, Jiongjiong; Kuang, Hanhui] Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ,MOA,Coll Hort & Forestry Sci, Key Lab Hort Crop Biol & Genet Improvement Cent R, Wuhan 430070, Peoples R China. [Baker, Barbara] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Baker, Barbara] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Kuang, HH (reprint author), Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ,MOA,Coll Hort & Forestry Sci, Key Lab Hort Crop Biol & Genet Improvement Cent R, Wuhan 430070, Peoples R China. EM kuangfile@mail.hzau.edu.cn RI Baker, Barbara/L-7198-2016 OI Baker, Barbara/0000-0002-1276-971X NR 9 TC 1 Z9 1 U1 3 U2 12 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1674-2052 EI 1752-9867 J9 MOL PLANT JI Mol. Plant. PD DEC 7 PY 2015 VL 8 IS 12 BP 1813 EP 1815 DI 10.1016/j.molp.2015.09.001 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CY3PD UT WOS:000366320900011 PM 26363271 ER PT J AU Jiang, ZL Dong, H Zheng, XB Marjani, SL Donovan, DM Chen, JB Tian, XC AF Jiang, Zongliang Dong, Hong Zheng, Xinbao Marjani, Sadie L. Donovan, David M. Chen, Jingbo Tian, Xiuchun (Cindy) TI mRNA Levels of Imprinted Genes in Bovine In Vivo Oocytes, Embryos and Cross Species Comparisons with Humans, Mice and Pigs SO SCIENTIFIC REPORTS LA English DT Article ID PORCINE PARTHENOGENETIC FETUSES; X-CHROMOSOME INACTIVATION; PREIMPLANTATION DEVELOPMENT; METHYLATION STATUS; EXPRESSION; CATTLE; SHEEP; SNRPN; SEQ; GENOME AB Twenty-six imprinted genes were quantified in bovine in vivo produced oocytes and embryos using RNA-seq. Eighteen were detectable and their transcriptional patterns were: largely decreased (MEST and PLAGL1); first decreased and then increased (CDKN1C and IGF2R); peaked at a specific stage (PHLDA2, SGCE, PEG10, PEG3, GNAS, MEG3, DGAT1, ASCL2, NNAT, and NAP1L5); or constantly low (DIRAS3, IGF2, H19 and RTL1). These patterns reflect mRNAs that are primarily degraded, important at a specific stage, or only required at low quantities. The mRNAs for several genes were surprisingly abundant. For instance, transcripts for the maternally imprinted MEST and PLAGL1, were high in oocytes and could only be expressed from the maternal allele suggesting that their genomic imprints were not yet established/recognized. Although the mRNAs detected here were likely biallelically transcribed before the establishment of imprinted expression, the levels of mRNA during these critical stages of development have important functional consequences. Lastly, we compared these genes to their counterparts in mice, humans and pigs. Apart from previously known differences in the imprinting status, the mRNA levels were different among these four species. The data presented here provide a solid reference for expression profiles of imprinted genes in embryos produced using assisted reproductive biotechnologies. C1 [Jiang, Zongliang; Tian, Xiuchun (Cindy)] Univ Connecticut, Dept Anim Sci, Ctr Regenerat Biol, Storrs, CT 06269 USA. [Dong, Hong; Zheng, Xinbao; Chen, Jingbo] Xinjiang Acad Anim Sci, Inst Anim Sci, Urumqi, Xinjiang, Peoples R China. [Marjani, Sadie L.] Cent Connecticut State Univ, Dept Biol, New Britain, CT 06050 USA. [Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. RP Chen, JB (reprint author), Xinjiang Acad Anim Sci, Inst Anim Sci, Urumqi, Xinjiang, Peoples R China. EM chenjb126@126.com; xiuchun.tian@uconn.edu FU USDA-ARS [1265-31000-091-02S]; USDA regional collaboration project [W2171] FX This project was supported by a grant from the USDA-ARS (1265-31000-091-02S) and the USDA regional collaboration project W2171. NR 46 TC 0 Z9 0 U1 1 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 7 PY 2015 VL 5 AR 17898 DI 10.1038/srep17898 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX8FI UT WOS:000365937700001 PM 26638780 ER PT J AU Woodall, CW Walters, BF Coulston, JW D'Amato, AW Domke, GM Russell, MB Sowers, PA AF Woodall, C. W. Walters, B. F. Coulston, J. W. D'Amato, A. W. Domke, G. M. Russell, M. B. Sowers, P. A. TI Monitoring Network Confirms Land Use Change is a Substantial Component of the Forest Carbon Sink in the eastern United States SO SCIENTIFIC REPORTS LA English DT Article ID USE HISTORY; CLIMATE-CHANGE; MANAGEMENT; VEGETATION; ACCUMULATION; DYNAMICS; POLICY AB Quantifying forest carbon (C) stocks and stock change within a matrix of land use (LU) and LU change is a central component of large-scale forest C monitoring and reporting practices prescribed by the Intergovernmental Panel on Climate Change (IPCC). Using a region-wide, repeated forest inventory, forest C stocks and stock change by pool were examined by LU categories. In eastern US forests, LU change is a substantial component of C sink strength (similar to 37% of forest sink strength) only secondary to that of C accumulation in forests remaining forest where their comingling with other LUs does not substantially reduce sink strength. The strongest sinks of forest C were study areas not completely dominated by forests, even when there was some loss of forest to agriculture/settlement/other LUs. Long-term LU planning exercises and policy development that seeks to maintain and/or enhance regional C sinks should explicitly recognize the importance of maximizing non-forest to forest LU changes and not overlook management and conservation of forests located in landscapes not currently dominated by forests. C1 [Woodall, C. W.; Walters, B. F.; Domke, G. M.; Sowers, P. A.] US Forest Serv, USDA, No Res Stn, Forest Inventory & Anal Program, St Paul, MN 55108 USA. [Coulston, J. W.] US Forest Serv, USDA, Southern Res Stn, Forest Inventory & Anal Program, Blacksburg, VA USA. [D'Amato, A. W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT USA. [Russell, M. B.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. RP Woodall, CW (reprint author), US Forest Serv, USDA, No Res Stn, Forest Inventory & Anal Program, St Paul, MN 55108 USA. EM cwoodall@fs.fed.us OI Domke, Grant/0000-0003-0485-0355 NR 29 TC 3 Z9 3 U1 1 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 7 PY 2015 VL 5 AR 17028 DI 10.1038/srep17028 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX6YP UT WOS:000365847900001 PM 26639409 ER PT J AU Xu, CX Jiao, C Zheng, Y Sun, HH Liu, WL Cai, XF Wang, XL Liu, S Xu, YM Mou, BQ Dai, SJ Fei, ZJ Wang, QH AF Xu, Chenxi Jiao, Chen Zheng, Yi Sun, Honghe Liu, Wenli Cai, Xiaofeng Wang, Xiaoli Liu, Shuang Xu, Yimin Mou, Beiquan Dai, Shaojun Fei, Zhangjun Wang, Quanhua TI De novo and comparative transcriptome analysis of cultivated and wild spinach SO SCIENTIFIC REPORTS LA English DT Article ID LONG NONCODING RNAS; GENE; GENOME; RESISTANCE; ANNOTATION; DISCOVERY; SEQUENCES; RESOURCE; OLERACEA; INFORMATION AB Spinach (Spinacia oleracea L.) is an economically important green leafy vegetable crop. In this study, we performed deep transcriptome sequencing for nine spinach accessions: three from cultivated S. oleracea, three from wild S. turkestanica and three from wild S. tetrandra. A total of approximately 100 million high-quality reads were generated, which were de novo assembled into 72,151 unigenes with a total length of 46.5 Mb. By comparing sequences of these unigenes against different protein databases, nearly 60% of them were annotated and 50% could be assigned with Gene Ontology terms. A total of 387 metabolic pathways were predicted from the assembled spinach unigenes. From the transcriptome sequencing data, we were able to identify a total of similar to 320,000 high-quality single nucleotide polymorphisms (SNPs). Phylogenetic analyses using SNPs as well as gene expression profiles indicated that S. turkestanica was more closely related to the cultivated S. oleracea than S. tetrandra. A large number of genes involved in responses to biotic and abiotic stresses were found to be differentially expressed between the cultivated and wild spinach. Finally, an interactive online database (http://www.spinachbase.org) was developed to allow the research community to efficiently retrieve, query, mine and analyze our transcriptome dataset. C1 [Xu, Chenxi; Cai, Xiaofeng; Wang, Xiaoli; Liu, Shuang; Dai, Shaojun; Wang, Quanhua] Shanghai Normal Univ, Dev & Collaborat Innovat Ctr Plant Germplasm Reso, Coll Life & Environm Sci, Shanghai 200234, Peoples R China. [Jiao, Chen; Zheng, Yi; Sun, Honghe; Liu, Wenli; Xu, Yimin; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Jiao, Chen] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Sun, Honghe] Natl Engn Res Ctr Vegetables, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China. [Mou, Beiquan] USDA, Agr Res Stn, Salinas, CA 93905 USA. [Fei, Zhangjun] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Fei, ZJ (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM zf25@cornell.edu; wangquanhua@shnu.edu.cn RI Zheng, Yi/F-6150-2016; OI Zheng, Yi/0000-0002-8042-7770; Fei, Zhangjun/0000-0001-9684-1450 FU Development and Collaborative Innovation Center of Shanghai [ZF1205]; Shanghai Set-Sail program [14YF1409400] FX This work was supported by Development and Collaborative Innovation Center of Shanghai [ZF1205] and Shanghai Set-Sail program [14YF1409400]. NR 44 TC 5 Z9 5 U1 2 U2 25 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 4 PY 2015 VL 5 AR 17706 DI 10.1038/srep17706 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX6OX UT WOS:000365821500003 PM 26635144 ER PT J AU Li, ZF Liu, YJ Feng, ZL Feng, HJ Klosterman, SJ Zhou, FF Zhao, LH Shi, YQ Zhu, HQ AF Li, Zhi-Fang Liu, Yi-Jie Feng, Zi-Li Feng, Hong-Jie Klosterman, Steven J. Zhou, Fang-Fang Zhao, Li-Hong Shi, Yong-Qiang Zhu, He-Qin TI VdCYC8, Encoding CYC8 Glucose Repression Mediator Protein, Is Required for Microsclerotia Formation and Full Virulence in Verticillium dahliae SO PLOS ONE LA English DT Article ID GENE-EXPRESSION; TRANSCRIPTIONAL REPRESSION; CANDIDA-ALBICANS; HYDROPHOBIN GENE; CROP-ROTATION; KINASE GENE; WILT; GROWTH; PATHOGENICITY; SSN6 AB Verticillium dahliae is the primary causal agent for Verticillium wilt disease on a diverse array of economically important crops, including cotton. In previous research, we obtained the low-pathogenicity mutant T286 from the T-DNA insertional mutant library of the highly virulent isolate Vd080 derived from cotton. In this study, the target disrupted gene VdCYC8 was identified by TAIL-PCR, encoding a homolog of CYC8 proteins involved in glucose repression. The deletion mutant Delta CYC8 exhibited several developmental deficiencies, including reduced microsclerotia formation, reduced sporulation, and slower growth. Moreover, compared with the wild type strain Vd080, the pathogenicity of strain Delta CYC8 was significantly decreased on cotton seedlings. However, the complementary mutants Delta CYC8-C led to restoration of the wild type phenotype or near wild type levels of virulence on cotton. Interestingly, pathogenicity of the strains was correlated with VdCYC8 gene expression levels in complemented mutants. Gene expression analyses in the wild type strain Vd080, the Delta CYC8-45 strain, and complemented strain Delta CYC8-C26 indicated that VdCYC8 regulates the transcription levels of several genes in V. dahliae that have roles in melanin and production. C1 [Li, Zhi-Fang; Liu, Yi-Jie; Feng, Zi-Li; Feng, Hong-Jie; Zhou, Fang-Fang; Zhao, Li-Hong; Shi, Yong-Qiang; Zhu, He-Qin] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. [Klosterman, Steven J.] ARS, USDA, Salinas, CA 93905 USA. RP Zhu, HQ (reprint author), Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. EM zhuheqin2012@163.com FU National Natural Science Foundation of China [31201466]; National High-Tech Program [2013AA102601] FX This work was financially supported by the National Natural Science Foundation of China (No. 31201466) and the National High-Tech Program (No. 2013AA102601).; This work was financially supported by the National Natural Science Foundation of China (No. 31201466) and the National High-tech program (No. 2013AA102601). We thank Prof. Dai Xiaofeng for providing A. tumefaciens strain AGL1 and plasmid pCTHyg. NR 52 TC 1 Z9 1 U1 7 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 3 PY 2015 VL 10 IS 12 AR e0144020 DI 10.1371/journal.pone.0144020 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX9QI UT WOS:000366040000044 PM 26633180 ER PT J AU Yu, M Ji, LX Neumann, DA Chung, DH Groom, J Westpheling, J He, C Schmitz, RJ AF Yu, Miao Ji, Lexiang Neumann, Drexel A. Chung, Dae-hwan Groom, Joseph Westpheling, Janet He, Chuan Schmitz, Robert J. TI Base-resolution detection of N-4-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite-sequencing SO NUCLEIC ACIDS RESEARCH LA English DT Article ID SINGLE-MOLECULE; METHYLATION VARIANTS; CYTOSINE METHYLATION; 5-METHYLCYTOSINE; RESTRICTION; N4-METHYLCYTOSINE; 5-HYDROXYMETHYLCYTOSINE; 5-CARBOXYLCYTOSINE; ARABIDOPSIS; METHYLOMES AB Restriction-modification (R-M) systems pose a major barrier to DNA transformation and genetic engineering of bacterial species. Systematic identification of DNA methylation in R-M systems, including N-6-methyladenine (6mA), 5-methylcytosine (5mC) and N-4-methylcytosine (4mC), will enable strategies to make these species genetically tractable. Although single-molecule, real time (SMRT) sequencing technology is capable of detecting 4mC directly for any bacterial species regardless of whether an assembled genome exists or not, it is not as scalable to profiling hundreds to thousands of samples compared with the commonly used next-generation sequencing technologies. Here, we present 4mC-Tet- assisted bisulfite-sequencing (4mC-TAB-seq), a next-generation sequencing method that rapidly and cost efficiently reveals the genome-wide locations of 4mC for bacterial species with an available assembled reference genome. In 4mC-TAB-seq, both cytosines and 5mCs are read out as thymines, whereas only 4mCs are read out as cytosines, revealing their specific positions throughout the genome. We applied 4mC-TAB-seq to study the methylation of a member of the hyperthermophilc genus, Caldicelluiosiruptor, inwhich 4mC-related restriction is a major barrier to DNA transformation from other species. In combination with MethylC-seq, both 4mC- and 5mC-containing motifs are identified which can assist in rapid and efficient genetic engineering of these bacteria in the future. C1 [Yu, Miao; He, Chuan] Univ Chicago, Howard Hughes Med Inst, Dept Chem, Chicago, IL 60637 USA. [Yu, Miao; He, Chuan] Univ Chicago, Howard Hughes Med Inst, Inst Biophys Dynam, Chicago, IL 60637 USA. [Ji, Lexiang] Univ Georgia, Inst Bioinformat, Athens, GA 30602 USA. [Neumann, Drexel A.; Chung, Dae-hwan; Groom, Joseph; Westpheling, Janet; Schmitz, Robert J.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Westpheling, Janet] USDA, BioEnergy Sci Ctr, Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA. RP Schmitz, RJ (reprint author), Univ Georgia, Dept Genet, Athens, GA 30602 USA. EM chuanhe@uchicago.edu; schmitz@uga.edu FU University of Georgia; National Institutes of Health [R01 HG006827]; BioEnergy Science Center, a U.S. Department of Energy Bioenergy Research Center - Office of Biological and Environmental Research in the DOE Office of Science FX University of Georgia [to R.J.S.]; National Institutes of Health [R01 HG006827 to C.H.]; The BioEnergy Science Center, a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science [to JW]; and M.Y. is a Howard Hughes Medical Institute predoctoral fellow; C.H. is an investigator of the Howard Hughes Medical Institute. Funding for open access charge: University of Georgia [to R.J.S.] NR 31 TC 2 Z9 2 U1 8 U2 20 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD DEC 2 PY 2015 VL 43 IS 21 AR e148 DI 10.1093/nar/gkv738 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CY4WZ UT WOS:000366410900011 PM 26184871 ER PT J AU Poly, F Serichantalergs, O Kuroiwa, J Pootong, P Mason, C Guerry, P Parker, CT AF Poly, Frederic Serichantalergs, Oralak Kuroiwa, Janelle Pootong, Piyarat Mason, Carl Guerry, Patricia Parker, Craig T. TI Updated Campylobacter jejuni Capsule PCR Multiplex Typing System and Its Application to Clinical Isolates from South and Southeast Asia SO PLOS ONE LA English DT Article ID POLYSACCHARIDE CONJUGATE VACCINE; IRRITABLE-BOWEL-SYNDROME; GENOME SEQUENCE; DIVERSITY; SEROTYPE; IDENTIFICATION; OUTBREAK; CHILDREN; THAILAND; STRAIN AB Campylobacter jejuni produces a polysaccharide capsule that is the major determinant of the Penner serotyping scheme. This passive slide agglutination typing system was developed in the early 1980's and was recognized for over two decades as the gold standard for C. jejuni typing. A preliminary multiplex PCR technique covering 17 serotypes was previously developed in order to replace this classic serotyping scheme. Here we report the completion of the multiplex PCR technology that is able to identify all the 47 Penner serotypes types known for C. jejuni. The number of capsule types represented within the 47 serotypes is 35. We have applied this method to a collection of 996 clinical isolates from Thailand, Cambodia and Nepal and were able to successfully determine capsule types of 98% of these. C1 [Poly, Frederic; Kuroiwa, Janelle; Guerry, Patricia] Naval Med Res Ctr, Enter Dis Dept, Silver Spring, MD 20910 USA. [Serichantalergs, Oralak; Pootong, Piyarat; Mason, Carl] Armed Forces Res Inst Med Sci, Bangkok 10400, Thailand. [Parker, Craig T.] ARS, USDA, Albany, CA USA. RP Poly, F (reprint author), Naval Med Res Ctr, Enter Dis Dept, Silver Spring, MD 20910 USA. EM frederic.m.poly.ctr@mail.mil OI MASON, CARL/0000-0002-3676-2811 FU U.S. Naval Medical Research and Development Command Work Unit [6000.RAD1.DA3.A0308]; US department of agriculture-Agricultural Research Service [CRIS 5325-42000-047]; Military Infectious Diseases Research Program, Walter Reed Army Institute of Research, Washington, DC FX This work was supported by U.S. Naval Medical Research and Development Command Work Unit 6000.RAD1.DA3.A0308 (FP JK PP PG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. US department of agriculture-Agricultural Research Service, CRIS 5325-42000-047 (CTP). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Military Infectious Diseases Research Program, Walter Reed Army Institute of Research, Washington, DC (OS PP CM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 2 Z9 2 U1 0 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 2 PY 2015 VL 10 IS 12 AR e0144349 DI 10.1371/journal.pone.0144349 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX8BI UT WOS:000365926300181 PM 26630669 ER PT J AU Lehotay, SJ Sapozhnikova, Y Han, LJ Johnston, JJ AF Lehotay, Steven J. Sapozhnikova, Yelena Han, Lijun Johnston, John J. TI Analysis of Nitrosamines in Cooked Bacon by QuEChERS Sample Preparation and Gas Chromatography-Tandem Mass Spectrometry with Backflushing SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE nitrosamines; bacon; QuEChERS; GC-MS/MS; dispersive-SPE ID VOLATILE N-NITROSAMINES; SOLID-PHASE EXTRACTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRESSURE CHEMICAL-IONIZATION; PROCESSED MEAT-PRODUCTS; NITROGEN CHEMILUMINESCENCE DETECTION; PESTICIDE-RESIDUES; FRIED BACON; DRINKING-WATER; WASTE-WATER AB Nitrites are added as a preservative to a variety of cured meats, including bacon, to kill bacteria, extend shelf life, and improve quality. During cooking, nitrites in the meat can be converted to carcinogenic nitrosamines (NAs), the formation of which is mitigated by the addition of antioxidants. In the past, the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS) monitored NAs in pan-fried bacon, but FSIS terminated monitoring of NAs in the 1990s due to the very low levels found. PSIS recently chose to conduct a risk assessment of NAs in cooked bacon to determine if current levels warrant routine monitoring of NM again. To meet FSIS needs, we developed, validated, and implemented a new method of sample preparation and analysis to test cooked bacon for five NAs of most concern, which consist of N-nitroso-dimethylamine, -diethylamine, -dibutylamine, -piperidine, and -pyrrolidine. Sample preparation was based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) approach and analysis by gas chromatography-tandem mass spectrometry. Ruggedness was improved markedly in the analysis of the complex fatty extracts by backflushing the guard column, injection liner, and half of the analytical column after every injection. Validation results were acceptable with recoveries of 70-120% and <20% RSDs for the five NM, with a reporting limit of 0.1 ng/g. NA concentrations in 48 samples were all <15 ng/g, with most <1 ng/g and many <0.1 ng/g. Also, microwave cooking of bacon gave slightly lower concentrations overall compared to pan-frying. C1 [Lehotay, Steven J.; Sapozhnikova, Yelena; Han, Lijun] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Han, Lijun] China Agr Univ, Coll Sci, Beijing 100193, Peoples R China. [Johnston, John J.] USDA, Food Safety & Inspect Serv, Off Publ Hlth Sci, Ft Collins, CO 80526 USA. RP Lehotay, SJ (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM Steven.Lehotay@ars.usda.gov FU National Natural Science Foundation of China [21177155] FX L.H.'s sabbatical funding was provided by the National Natural Science Foundation of China (No. 21177155). Mention of brand or firm names does not constitute an endorsement by the U.S. Department of Agriculture above others of a similar nature not mentioned. NR 54 TC 2 Z9 2 U1 12 U2 48 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 2 PY 2015 VL 63 IS 47 BP 10341 EP 10351 DI 10.1021/acs.jafc.5b04527 PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CX8CK UT WOS:000365930000018 PM 26542769 ER PT J AU Hussein, HE Scoles, GA Ueti, MW Suarez, CE Adham, FK Guerrero, FD Bastos, RG AF Hussein, Hala E. Scoles, Glen A. Ueti, Massaro W. Suarez, Carlos E. Adham, Fatma K. Guerrero, Felix D. Bastos, Reginaldo G. TI Targeted silencing of the Aquaporin 2 gene of Rhipicephalus (Boophilus) microplus reduces tick fitness SO PARASITES & VECTORS LA English DT Article DE Rhipicephalus (Boophilus) microplus; Aquaporin; Tick fitness; RNA interference; Gene silencing; Babesia bovis ID BABESIA-BOVIS; CATTLE TICK; VACCINE; INFESTATIONS; INFORMATION; IXODIDAE; GAMMA; ACARI AB Background: Ticks are blood-feeding arthropods that can affect human and animal health both directly by blood-feeding and indirectly by transmitting pathogens. The cattle tick Rhipicephalus (Boophilus) microplus is one of the most economically important ectoparasites of bovines worldwide and it is responsible for the transmission of the protozoan Babesia bovis, the etiological agent of bovine babesiosis. Aquaporins (AQPs) are water channel proteins implicated in physiological mechanisms of osmoregulation. Members of the AQP family are critical for blood-feeding arthropods considering the extreme osmoregulatory changes that occur during their feeding. We investigated the pattern of expression of a newly identified AQP2 gene of R. microplus (RmAQP2) in different tick tissues and stages. We also examined in vivo the biological implications of silencing expression of RmAQP2 silencing during tick feeding on either uninfected or B. bovis-infected cattle. Methods: In silico gene analyses were performed by multiple alignments of amino acid sequences and topology prediction. Levels of RmAQP2 transcripts in different tick tissues and stages were analyzed by reverse transcriptase quantitative PCR. Patterns of expression of RmAQP2 protein were investigated by immunoblots. Gene silencing was performed by RNA interference and in vivo functional analyses carried out by feeding ticks on either uninfected or B. bovis-infected cattle. Results: RmAQP2 transcripts were found in unfed larvae, engorged nymphs, and salivary glands and guts of partially engorged females; however, of all tick tissues and stages examined, RmAQP2 protein was found only in salivary glands of partially engorged females. RmAQP2 silencing significantly reduced tick fitness and completely abrogated protein expression. The effect of RmAQP2 silencing on fitness was more pronounced in females fed on a B. bovis-infected calf than in ticks fed on an uninfected calf and none of their larval progeny survived. Conclusions: Collectively, considering the gene expression and tick fitness data, we conclude that RmAQP2 is critical for tick blood feeding and may be a suitable candidate target for the development of novel strategies to control R. microplus and tick-borne parasites. C1 [Hussein, Hala E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Hussein, Hala E.; Adham, Fatma K.] Cairo Univ, Fac Sci, Dept Entomol, Giza 12613, Egypt. [Scoles, Glen A.; Ueti, Massaro W.; Suarez, Carlos E.] Washington State Univ, ARS, USDA, Anim Dis Res Unit, Pullman, WA 99164 USA. [Guerrero, Felix D.] ARS, USDA, Knipling Bushland US Livestock Insect Res Lab, Kerrville, TX 78028 USA. [Guerrero, Felix D.] ARS, USDA, Vet Pest Genom Ctr, Kerrville, TX 78028 USA. [Bastos, Reginaldo G.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. RP Bastos, RG (reprint author), Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. EM rgbastos@vetmed.wsu.edu FU USDA-ARS-CRIS [5348-32000-034-00D]; Egyptian Ministry of High Education FX We thank Kathy Mason, Ralph Horn and James Allison for excellent technical assistance. This work was supported by USDA-ARS-CRIS project number 5348-32000-034-00D. H. E. Hussein was financially supported by the Egyptian Ministry of High Education. NR 38 TC 5 Z9 5 U1 1 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD DEC 2 PY 2015 VL 8 AR 618 DI 10.1186/s13071-015-1226-2 PG 12 WC Parasitology SC Parasitology GA CX2DS UT WOS:000365506200002 PM 26626727 ER PT J AU Cutts, BB Munoz-Erickson, TA Shutters, ST AF Cutts, Bethany B. Munoz-Erickson, Tischa A. Shutters, Shade T. TI Public Representation in Water Management-A Network Analysis of Organization and Public Perceptions in Phoenix, Arizona SO SOCIETY & NATURAL RESOURCES LA English DT Article DE collaboration; public information; social network analysis; urban; water resource conservation ID DEPENDENCY; CAMPAIGNS; ISSUES; MODELS AB To better accomplish their mission of an informed public, environmental education organizations often exchange ideas, share financing, and distribute overhead through collaboration. Yet it remains to be seen whether benefits of these collaborations extend to the public. We examine two possible benefits: the ability of the organizations to act as representatives of the public interest, and equitable access to environmental educational materials. We model patterns of public access to water-related education across organizations using two surveys in metropolitan Phoenix, AZ. This enables the study of interorganizational social networks and public outcomes. Results support the idea that environmental education organizations could provide a credible proxy for direct citizen participation. However, not all organizations are equivalently engaged with historically underrepresented groups like women, minority racial and ethnic groups, and those who rent their home. The implications for more inclusive environmental policy decisions are discussed. C1 [Cutts, Bethany B.] Univ llinois Urbana Champaign, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Munoz-Erickson, Tischa A.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR USA. [Shutters, Shade T.] Arizona State Univ, Global Secur Initiat, Tempe, AZ USA. RP Cutts, BB (reprint author), Univ llinois Urbana Champaign, Dept Nat Resources & Environm Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM bcutts@illinois.edu FU National Science Foundation [SES-0951366]; Decision Center for a Desert City II: Urban Climate Adaptation; USDA through Networks and Just Sustainability in Urban and Rural Agricultural Landscapes [ILLU-875-919: 7] FX This material is based upon work supported by the National Science Foundation under grant SES-0951366, Decision Center for a Desert City II: Urban Climate Adaptation, and by the USDA through ILLU-875-919: 7 Networks and Just Sustainability in Urban and Rural Agricultural Landscapes. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation or U.S. Department of Agriculture. NR 44 TC 0 Z9 0 U1 4 U2 15 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0894-1920 EI 1521-0723 J9 SOC NATUR RESOUR JI Soc. Nat. Resour. PD DEC 2 PY 2015 VL 28 IS 12 BP 1340 EP 1357 DI 10.1080/08941920.2015.1020581 PG 18 WC Environmental Studies; Planning & Development; Sociology SC Environmental Sciences & Ecology; Public Administration; Sociology GA CV5TJ UT WOS:000364333800006 ER PT J AU George, AD Thompson, FR Faaborg, J AF George, Andrew D. Thompson, Frank R., III Faaborg, John TI Using LiDAR and remote microclimate loggers to downscale near-surface air temperatures for site-level studies SO REMOTE SENSING LETTERS LA English DT Article ID CLIMATE-CHANGE IMPACTS; COMPLEX TERRAIN; BIODIVERSITY; CONSERVATION; PARAMETERS; PATTERNS AB A spatial mismatch exists between regional climate models and conditions experienced by individual organisms. We demonstrate an approach to downscaling air temperatures for site-level studies using airborne LiDAR data and remote microclimate loggers. In 2012-2013, we established a temperature logger network in the forested region of central Missouri, USA, and obtained sub-hourly meteorological measurements from a centrally located weather station. We then used linear mixed models within an information theoretic approach to evaluate hourly and seasonal effects of insolation, vegetation structure, elevation, and meteorological measurements on near-surface air temperatures. The best-supported models predicted fine-scale temperatures with high accuracy during both the winter and growing seasons. We recommend that researchers consider the scales relevant to specific applications when using our approach to develop site-specific spatio-temporal models. C1 [George, Andrew D.; Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] USDA, No Res Stn, Forest Serv, Columbia, MO USA. RP George, AD (reprint author), Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. EM adgggb@mail.missouri.edu FU U.S.D.A. Forest Service Northern Research Station; University of Missouri FX Funding for this project was provided by the U.S.D.A. Forest Service Northern Research Station and the University of Missouri. NR 32 TC 0 Z9 0 U1 3 U2 18 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 2150-704X EI 2150-7058 J9 REMOTE SENS LETT JI Remote Sens. Lett. PD DEC 2 PY 2015 VL 6 IS 12 BP 924 EP 932 DI 10.1080/2150704X.2015.1088671 PG 9 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA CS9YX UT WOS:000362451300002 ER PT J AU Ayele, GK Gessess, AA Addisie, MB Tilahun, SA Tenessa, DB Langendoen, EJ Steenhuis, TS Nicholson, CF AF Ayele, Getaneh K. Gessess, Azalu A. Addisie, Meseret B. Tilahun, Seifu A. Tenessa, Daregot B. Langendoen, Eddy J. Steenhuis, Tammo S. Nicholson, Charles F. TI The economic cost of upland and gully erosion on subsistence agriculture for a watershed in the Ethiopian highlands SO AFRICAN JOURNAL OF AGRICULTURAL AND RESOURCE ECONOMICS-AFJARE LA English DT Article DE Erosion; replacement cost; gully erosion; Ethiopia; East Africa ID SOIL-EROSION; SEDIMENT; NITROGEN AB This study quantifies the costs of erosion in a watershed of the Ethiopian highlands over a two-year period, accounting for topsoil nutrient losses, time costs due to disrupted travel networks, and the value of lost animals and trees. We use a nutrient replacement cost to value topsoil nutrient depletion, daily wage rate to monetise the opportunity cost of labour due to gully erosion, and local market prices to quantify the lost animals and cash crop trees. The total cost of soil erosion in the watershed during the two years was over $18 000, a cost of $22 per ha per year, $17 per person per year or about 19% of per capita income. The nutrient depletion from topsoil comprised only 42% of total costs. Given these large costs, remediation measures focused on participatory community-based efforts should be evaluated to reduce the severity of soil erosion and its associated effects. C1 [Nicholson, Charles F.] Penn State Univ, Dept Supply Chain & Informat Syst, University Pk, PA 16802 USA. [Ayele, Getaneh K.; Gessess, Azalu A.; Addisie, Meseret B.; Tilahun, Seifu A.] Bahir Dar Univ, Fac Civil & Water Resources Engn, Bahir Dar, Ethiopia. [Tenessa, Daregot B.] Bahir Dar Univ, Coll Business & Econ, Dept Econ, Bahir Dar, Ethiopia. [Langendoen, Eddy J.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Steenhuis, Tammo S.] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. RP Nicholson, CF (reprint author), Penn State Univ, Dept Supply Chain & Informat Syst, University Pk, PA 16802 USA. EM getanehk89@yahoo.com; Azalualebachew@gmail.com; meseret.belachew21@gmail.com; sat86@cornell.edu; daregot21@gmail.com; eddy.langendoen@ars.usda.gov; tss1@cornell.edu; cfn10@psu.edu NR 38 TC 1 Z9 1 U1 6 U2 8 PU AFRICAN ASSOC AGRICULTURAL ECONOMISTS PI NAIROBI PA PO BOX 62882-00200, NAIROBI, 00000, KENYA SN 1993-3738 J9 AFR J AGRIC RESOUR E JI Afr. J. Agric. Resour. Econ.-AFJARE PD DEC PY 2015 VL 10 IS 4 BP 265 EP 278 PG 14 WC Agricultural Economics & Policy SC Agriculture GA DT8HR UT WOS:000381731300001 ER PT J AU Verburg, PH Crossman, N Ellis, EC Heinimann, A Hostert, P Mertz, O Nagendra, H Sikor, T Erb, KH Golubiewski, N Grau, R Grove, M Konate, S Meyfroidt, P Parker, DC Chowdhury, RR Shibata, H Thomson, A Zhen, L AF Verburg, Peter H. Crossman, Neville Ellis, Erle C. Heinimann, Andreas Hostert, Patrick Mertz, Ole Nagendra, Harini Sikor, Thomas Erb, Karl-Heinz Golubiewski, Nancy Grau, Ricardo Grove, Morgan Konate, Souleymane Meyfroidt, Patrick Parker, Dawn C. Chowdhury, Rinku Roy Shibata, Hideaki Thomson, Allison Zhen, Lin TI Land system science and sustainable development of the earth system: A global land project perspective SO ANTHROPOCENE LA English DT Article DE Land use; Land cover; Land systems; Ecosystem; Human-environment systems; Global Land Project ID ECOSYSTEM SERVICES; COVER CHANGE; SOCIOECOLOGICAL SYSTEMS; ENVIRONMENTAL-CHANGE; FOREST TRANSITION; CLIMATE-CHANGE; FOOD SECURITY; USE INTENSITY; TRADE-OFFS; USE MODELS AB Land systems are the result of human interactions with the natural environment. Understanding the drivers, state, trends and impacts of different land systems on social and natural processes helps to reveal how changes in the land system affect the functioning of the socio-ecological system as a whole and the tradeoff these changes may represent. The Global Land Project has led advances by synthesizing land systems research across different scales and providing concepts to further understand the feedbacks between social-and environmental systems, between urban and rural environments and between distant world regions. Land system science has moved from a focus on observation of change and understanding the drivers of these changes to a focus on using this understanding to design sustainable transformations through stakeholder engagement and through the concept of land governance. As land use can be seen as the largest geo-engineering project in which mankind has engaged, land system science can act as a platform for integration of insights from different disciplines and for translation of knowledge into action. (C) 2015 The Authors. Published by Elsevier Ltd. C1 [Verburg, Peter H.] Vrije Univ Amsterdam, Environm Geog Grp, de Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands. [Crossman, Neville] CSIRO Land & Water, Private Bag 2, Glen Osmond, SA 5064, Australia. [Ellis, Erle C.] Univ Maryland Baltimore Cty, Dept Geog & Environm Syst, Baltimore, MD 21250 USA. [Heinimann, Andreas] Univ Bern, CDE, Hallerstr 10, CH-3012 Bern, Switzerland. [Heinimann, Andreas] Univ Bern, Inst Geog, Hallerstr 10, CH-3012 Bern, Switzerland. [Hostert, Patrick] Humboldt Univ, Dept Geog, Berlin, Germany. [Hostert, Patrick] Humboldt Univ, Integrat Res Inst Transformat Human Environm Syst, Berlin, Germany. [Mertz, Ole] Univ Copenhagen, Dept Geosci & Nat Resource Management, Copenhagen, Denmark. [Nagendra, Harini] Azim Premji Univ, Sch Dev, Bangalore, Karnataka, India. [Sikor, Thomas] Univ East Anglia, Sch Int Dev, Norwich, Norfolk, England. [Erb, Karl-Heinz] Alpen Adria Univ Klagenfurt Vienna Graz, Inst Social Ecol Vienna SEC, Schottenfeldgasse 29, A-1070 Vienna, Austria. [Golubiewski, Nancy] Minist Environment Manatu Mo Te Taiao, 23 Kate Sheppard Pl,POB 10362, Wellington 6143, New Zealand. [Grau, Ricardo] Univ Nacl Tucuman, CONICET, Inst Ecol Reg, CC34, RA-4107 San Miguel De Tucuman, Argentina. [Grove, Morgan] US Forest Serv, Northern Res Stn, USDA, 5200 Westland Blvd,TRC 171, Baltimore, MD 21227 USA. [Konate, Souleymane] Univ Abobo Adjame, UFR SN CRE, IUCN Cent & West Africa, 02 BP 801, Abidjan 02, Cote Ivoire. [Meyfroidt, Patrick] FRS FNRS, Louvain, Belgium. [Meyfroidt, Patrick] Catholic Univ Louvain, Earth & Life Inst, Georges Lemaitre Ctr Earth & Climate Res TECLIM, Louvain, Belgium. [Parker, Dawn C.] Univ Waterloo, Sch Planning, 200 Univ Ave West, Waterloo, ON, Canada. [Chowdhury, Rinku Roy] Clark Univ, Grad Sch Geog, 950 Main St, Worcester, MA 01610 USA. [Shibata, Hideaki] Hokkaido Univ, Field Sci Ctr No Biosphere, Kita Ku, Kita 9,Nishi 9, Sapporo, Hokkaido 0600809, Japan. [Thomson, Allison] Alliance Sustainable Agr, Field Market, 777 N Capitol St NE,Suite 803, Washington, DC 20002 USA. [Zhen, Lin] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, 11A Datun Rd, Beijing 100101, Peoples R China. RP Verburg, PH (reprint author), Vrije Univ Amsterdam, Environm Geog Grp, de Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands. EM Peter.Verburg@vu.nl RI Verburg, Peter/A-8469-2010; Crossman, Neville/G-5433-2010; OI Verburg, Peter/0000-0002-6977-7104; Meyfroidt, Patrick/0000-0002-1047-9794; Heinimann, Andreas/0000-0001-8905-8169; Crossman, Neville/0000-0002-8002-3450; Mertz, Ole/0000-0002-3876-6779 NR 142 TC 10 Z9 11 U1 11 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 2213-3054 J9 ANTHROPOCENE JI Anthropocene PD DEC PY 2015 VL 12 BP 29 EP 41 DI 10.1016/j.ancene.2015.09.004 PG 13 WC Geosciences, Multidisciplinary SC Geology GA DU0OE UT WOS:000381903600004 ER PT J AU Baldwin, RA Meinerz, R Jantz, HE Witmer, GW AF Baldwin, Roger A. Meinerz, Ryan Jantz, Holly E. Witmer, Gary W. TI IMPACT OF CAPTURE AND TRANSPORTATION METHODS ON SURVIVAL OF SMALL RODENTS DURING RELOCATION EVENTS SO SOUTHWESTERN NATURALIST LA English DT Article ID NORTHERN POCKET GOPHERS; STRESS; NESTS; VOLE; MICE AB Capture and transportation of wild rodents is needed to supply study animals for laboratory or enclosure studies and for translocation of threatened and endangered species. Stress of captured rodents must be minimized to maximize survival. Methods to limit stress include minimizing capture and transportation durations, providing sufficiently sized housing with adequate nesting materials and foods, and ensuring that animals are maintained in comfortable environmental conditions. We utilized these techniques to capture and transport California voles (Microtus californicus) and pocket gophers (Thomomys bottae) from California to Colorado to determine their rate of survival during this process. We captured pocket gophers through livetrapping; burrow excavation substantially reduced capture and holding times for voles. All 50 voles and 88 of 91 pocket gophers were still alive and in good condition 2 weeks postarrival. The techniques and materials described should provide a useful framework for other wild rodents as well. C1 [Baldwin, Roger A.; Meinerz, Ryan] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Jantz, Holly E.] Univ Calif Cooperat Extens, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. [Witmer, Gary W.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Baldwin, RA (reprint author), Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. EM rabaldwin@ucdavis.edu FU Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture; University of California Statewide Integrated Pest Management Program FX Research was funded by the Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture, and the University of California Statewide Integrated Pest Management Program. We extend a special thank you to F. Castaneda and numerous farm workers for Sea Mist/Ocean Mist Farms for providing access to their property and field assistance for the project. We also thank J. Castro and the Pala Band of Mission Indians, and R. Weinstock and Gallo Family Vineyards for access to property for pocket gopher trapping. Numerous individuals provided very valuable insight into potential transportation methods for small rodents. We also thank R. Moulton for translating the abstract into Spanish. NR 10 TC 0 Z9 0 U1 0 U2 0 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 EI 1943-6262 J9 SOUTHWEST NAT JI Southw. Natural. PD DEC PY 2015 VL 60 IS 4 BP 385 EP U156 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DO0BF UT WOS:000377443700016 ER PT J AU Triplehorn, CA Thomas, DB AF Triplehorn, Charles A. Thomas, Donald B. TI A REVISION OF ELEODES SUBGENUS LITHELEODES BLAISDELL (COLEOPTERA: TENEBRIONIDAE) SO COLEOPTERISTS BULLETIN LA English DT Article DE taxonomy; darkling beetles; Nearctic; Chuck Triplehorn ID ESCHSCHOLTZ COLEOPTERA; BEETLES COLEOPTERA AB Litheleodes Blaisdell, a subgenus of Eleodes Eschscholtz, is reviewed with diagnoses, distributions, and nomenclatural notes for the nine included species. A type species, Blaps extricata Say, is herein designated, and the subgeneric definition is updated to differentiate it from related subgenera. The species, distributed over the western USA and adjacent parts of Canada and Mexico, are keyed and illustrated. C1 [Triplehorn, Charles A.] Ohio State Univ, Museum Biol Div, 1315 Kinnear Rd, Columbus, OH 43212 USA. [Thomas, Donald B.] USDA ARS, Cattle Fever Tick Res Lab, Edinburg, TX 78596 USA. RP Triplehorn, CA (reprint author), Ohio State Univ, Museum Biol Div, 1315 Kinnear Rd, Columbus, OH 43212 USA. EM donald.thomas@ars.usda.gov FU National Science Foundation FX The authors are grateful to the curators of the mentioned collections for access to the material in their care. The senior author wishes to acknowledge the support of the National Science Foundation. Aaron Smith provided a very helpful and constructive criticism of the manuscript. We especially thank our colleague Melinda May for preparation of the maps. Mention of a proprietary product is for informational purposes only and does not constitute endorsement by the USDA. NR 36 TC 0 Z9 0 U1 1 U2 1 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD DEC PY 2015 VL 69 BP 11 EP 21 DI 10.1649/0010-065X-69.mo4.11 PG 11 WC Entomology SC Entomology GA DE8OH UT WOS:000370895200002 ER PT J AU Thomas, DB AF Thomas, Donald B. TI CHASELEODES THOMAS: A NEW SUBGENUS OF ELEODES ESCHSCHOLTZ (COLEOPTERA: TENEBRIONIDAE) FROM THE CENTRAL PLATEAU OF MEXICO SO COLEOPTERISTS BULLETIN LA English DT Article DE taxonomy; darkling beetles; Eleodes connatus; Eleodes curtus; Chuck Triplehorn AB Chaseleodes Thomas, a new subgenus of Eleodes Eschscholtz, is described and proposed to hold two species from central Mexico, Eleodes connatus Solier and Eleodes curtus Champion (designated as type species). A key, map, illustrations, and diagnoses are provided for the species. Characters that in combination define the new subgenus include the wedge-shaped elytron, broadly based epipleuron, and the female valvifers. The subgenus is named in honor of Dr. Chas. A. Triplehorn. C1 [Thomas, Donald B.] USDA ARS, Cattle Fever Tick Res Lab, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. RP Thomas, DB (reprint author), USDA ARS, Cattle Fever Tick Res Lab, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM donald.thomas@ars.usda.gov NR 6 TC 0 Z9 0 U1 0 U2 0 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD DEC PY 2015 VL 69 BP 122 EP 126 DI 10.1649/0010-065X-69.mo4.122 PG 5 WC Entomology SC Entomology GA DE8OH UT WOS:000370895200013 ER PT J AU Hoagland, BW Buthod, A Arbour, D AF Hoagland, Bruce W. Buthod, Amy Arbour, David TI Additions to the Vascular Flora of the Red Slough Wildlife Management Area, McCurtain County, Oklahoma SO CASTANEA LA English DT Article DE Flora; inventory; Oklahoma; rare taxa; Upper Gulf Coastal Plain ID CONSERVATION AB The Red Slough Wildlife Management Area (7,800 ha) is located on the West Gulf Coastal Plain in southeastern Oklahoma. The inventory was motivated by land acquisitions and is intended to augment a 1999 inventory. We report an additional 202 taxa of vascular plants, with 186 species, 16 infraspecific taxa, 158 genera, and 68 families. This is a 62% increase in the number of taxa previously listed for the site. The largest families were the Poaceae (with 35 taxa), Asteraceae (20 taxa), and Fabaceae (18 taxa). Thirty-one nonnative taxa and 19 taxa tracked by the Oklahoma Natural Heritage Inventory were encountered. C1 [Hoagland, Bruce W.; Buthod, Amy] Oklahoma Biol Survey, 111 East Chesapeake St, Norman, OK 73019 USA. [Hoagland, Bruce W.] Univ Oklahoma, Dept Geog & Environm Sustainabil, Norman, OK 73019 USA. [Arbour, David] US Forest Serv, Broken Bow, OK 74728 USA. RP Hoagland, BW (reprint author), Oklahoma Biol Survey, 111 East Chesapeake St, Norman, OK 73019 USA. EM bhoagland@ou.edu FU United States Forest Service FX This project was funded by the United States Forest Service. NR 20 TC 0 Z9 0 U1 2 U2 2 PU SOUTHERN APPALACHIAN BOTANICAL SOC, NEWBERRY COLL PI NEWBERRY PA DEPT BIOLOGY, C/O CHARLES N HORN, SECRETARY-TREASURER, 2100 COLLEGE ST, NEWBERRY, SC 29108 USA SN 0008-7475 EI 1938-4386 J9 CASTANEA JI Castanea PD DEC PY 2015 VL 80 IS 4 BP 253 EP 261 DI 10.2179/15-061 PG 9 WC Plant Sciences SC Plant Sciences GA DI7CM UT WOS:000373656700003 ER PT J AU Li, DY Zhao, TJ Shi, JC Bindlish, R Jackson, TJ Peng, B An, M Han, B AF Li, Dongyang Zhao, Tianjie Shi, Jiancheng Bindlish, Rajat Jackson, Thomas J. Peng, Bin An, Meng Han, Bo TI First Evaluation of Aquarius Soil Moisture Products Using In Situ Observations and GLDAS Model Simulations SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Aquarius; global land data assimilation system (GLDAS); international soil moisture network (ISMN); soil climate analysis network (SCAN); soil moisture (SM); validation ID MICROWAVE SCANNING RADIOMETER; DATA ASSIMILATION SYSTEM; AMSR-E; LAND-SURFACE; SATELLITE-OBSERVATIONS; MEDICAL STATISTICS; TIBETAN PLATEAU; GLOBAL-SCALE; WATER CYCLE; NETWORK AB L-band satellite remote sensing is one of the most promising techniques for global monitoring of soil moisture (SM). In addition to soil moisture and ocean salinity (SMOS) SM products, another global SM product has been developed using Aquarius, which is the first operational active/passive L-band satellite sensor. The spatial resolution of Aquarius SM products is about 100 km, which presents more challenges to the ground-based validation. This study explores approaches to validate and evaluate the Aquarius SM products in terms of their spatial and temporal distributions, through synergistic use of in situ measurements and model products from the global land data assimilation system (GLDAS). A dense soil moisture/temperature monitoring network over the central Tibetan plateau (CTP-SMTMN) and sparse stations from the soil climate analysis network (SCAN) over United States are used for the reliability assessment of Aquarius SM products. Results show that the Aquarius SM captures the spatial-temporal variability of CTP-SMTMN reference dataset with an overall RMSD of 0.078 m(3) . m(-3) and correlation coefficient of 0.767. The comparison results with reference to SCAN datasets suggest that the RMSD can reach to the target value of 0.04 m(3) . m(-3) over specific stations, but the impacts from different orbits, seasons, and land cover types are also found to be significant. The comparison between Aquarius retrievals and GLDAS/common land model (CLM) simulations presents a general well statistical agreement with correlation coefficients above 0.5 for most terrestrial areas. These results are considered to support the use of Aquarius SM products in future applications. C1 [Li, Dongyang; Zhao, Tianjie; Shi, Jiancheng; Peng, Bin] Chinese Acad Sci, State Key Lab Remote Sensing Sci, Inst Remote Sensing & Digital Earth RADI, Beijing 100101, Peoples R China. [Li, Dongyang; Zhao, Tianjie; Shi, Jiancheng; Peng, Bin] Joint Ctr Global Change Studies, Beijing 100875, Peoples R China. [Bindlish, Rajat; Jackson, Thomas J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [An, Meng; Han, Bo] China Acad Space Technol, Beijing Inst Spacecraft Syst Engn, Beijing 100094, Peoples R China. RP Zhao, TJ (reprint author), Chinese Acad Sci, State Key Lab Remote Sensing Sci, Inst Remote Sensing & Digital Earth RADI, Beijing 100101, Peoples R China. EM lidy@radi.ac.cn; zhaotj@radi.ac.cn; shijc@radi.ac.cn; rajat.bindlish@ars.usda.gov; tom.jackson@ars.usda.gov; pengbin@radi.ac.cn FU CAS/SAFEA International Partnership Program for Creative Research Teams [KZZD-EW-TZ-09]; National Natural Science Foundation of China [41301396]; National Key Basic Research Program [2015CB953701]; Young Scholar Fund of Director of Institute of Remote Sensing and Digital Earth FX This work was supported in part by the CAS/SAFEA International Partnership Program for Creative Research Teams under Grant KZZD-EW-TZ-09 and in part by the National Natural Science Foundation of China (41301396), the National Key Basic Research Program (2015CB953701) and the Young Scholar Fund of Director of Institute of Remote Sensing and Digital Earth. (Corresponding author: Tianjie Zhao.) NR 79 TC 2 Z9 2 U1 11 U2 14 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD DEC PY 2015 VL 8 IS 12 BP 5511 EP 5525 DI 10.1109/JSTARS.2015.2452955 PG 15 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA DE3HY UT WOS:000370519700013 ER PT J AU Bellugi, D Milledge, DG Dietrich, WE Perron, JT McKean, J AF Bellugi, Dino Milledge, David G. Dietrich, William E. Perron, J. Taylor McKean, Jim TI Predicting shallow landslide size and location across a natural landscape: Application of a spectral clustering search algorithm SO JOURNAL OF GEOPHYSICAL RESEARCH-EARTH SURFACE LA English DT Article ID OREGON COAST RANGE; SURFACE HYDROLOGIC RESPONSE; SLOPE-STABILITY; DEBRIS FLOWS; UNCHANNELED CATCHMENT; CHANNEL INITIATION; FOREST MANAGEMENT; RUNOFF GENERATION; HAZARD ASSESSMENT; COOS-BAY AB Predicting shallow landslide size and location across landscapes is important for understanding landscape form and evolution and for hazard identification. We test a recently developed model that couples a search algorithm with 3-D slope stability analysis that predicts these two key attributes in an intensively studied landscape with a 10 year landslide inventory. We use process-based submodels to estimate soil depth, root strength, and pore pressure for a sequence of landslide-triggering rainstorms. We parameterize submodels with field measurements independently of the slope stability model, without calibrating predictions to observations. The model generally reproduces observed landslide size and location distributions, overlaps 65% of observed landslides, and of these predicts size to within factors of 2 and 1.5 in 55% and 28% of cases, respectively. Five percent of the landscape is predicted unstable, compared to 2% recorded landslide area. Missed landslides are not due to the search algorithm but to the formulation and parameterization of the slope stability model and inaccuracy of observed landslide maps. Our model does not improve location prediction relative to infinite-slope methods but predicts landslide size, improves process representation, and reduces reliance on effective parameters. Increasing rainfall intensity or root cohesion generally increases landslide size and shifts locations down hollow axes, while increasing cohesion restricts unstable locations to areas with deepest soils. Our findings suggest that shallow landslide abundance, location, and size are ultimately controlled by covarying topographic, material, and hydrologic properties. Estimating the spatiotemporal patterns of root strength, pore pressure, and soil depth across a landscape may be the greatest remaining challenge. C1 [Bellugi, Dino; Perron, J. Taylor] MIT, Dept Earth Atmospher & Planetary Sci, Cambridge, MA USA. [Milledge, David G.] Univ Durham, Dept Geog, Durham, England. [Dietrich, William E.] Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 USA. [McKean, Jim] US Forest Serv, Rocky Mt Res Stn, USDA, Boise, ID USA. RP Bellugi, D (reprint author), MIT, Dept Earth Atmospher & Planetary Sci, Cambridge, MA USA. EM dinob@mit.edu OI Milledge, David/0000-0003-4077-4898 FU United States Forest Service [09-JV-11221634-233]; NSF [NSF-EAR-0828047]; NASA [ROSES 09-IDS09-0049]; NERC [NER/S/A/2004/12248] FX This research was financially supported by grants from the United States Forest Service (09-JV-11221634-233), NSF (NSF-EAR-0828047), NASA (ROSES 09-IDS09-0049), and NERC (NER/S/A/2004/12248). We thank Kevin Schmidt for providing root cohesion and soil depth data, Dave Montgomery for providing landslide dimensions data, and Maria Cristina Rulli for providing assistance with the hydrological modeling. All data sets used in this study have been previously published, please see citations in the text. Reviews by Alex Densmore, John Buffington, Katerina Michaelides, Oliver Korup, and four anonymous referees considerably improved this manuscript. NR 117 TC 1 Z9 1 U1 7 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-9003 EI 2169-9011 J9 J GEOPHYS RES-EARTH JI J. Geophys. Res.-Earth Surf. PD DEC PY 2015 VL 120 IS 12 BP 2552 EP 2585 DI 10.1002/2015JF003520 PG 34 WC Geosciences, Multidisciplinary SC Geology GA DD5EH UT WOS:000369944900006 ER PT J AU Phillips, WS Kieran, SR Zasada, IA AF Phillips, Wendy S. Kieran, Shannon Rose Zasada, Inga A. TI The Relationship between Temperature and Development in Globodera ellingtonae SO JOURNAL OF NEMATOLOGY LA English DT Article DE degree-day; development; Globodera; potato; soil temperature ID POTATO-CYST-NEMATODE; ROSTOCHIENSIS; POPULATIONS; HETERODERIDAE; PALLIDA; OREGON AB A new cyst nematode species, Globodera ellingtonae, was recently described from populations in Oregon and Idaho. This nematode has been shown to reproduce on potato. Because of this nematode's close relationship to the potato cyst nematodes, G. rostochiensis and G. pallida, an understanding of the risk of its potential spread, including prediction of potential geographical distribution, is required. To determine the development of G. ellingtonae under different temperatures, we conducted growth chamber experiments over a range of temperatures (10.0 degrees C to 26.5 degrees C) and tracked length of time to various developmental stages, including adult females bearing the next generation of eggs. Both the time to peak population densities of G. ellingtonae life stages and their duration in roots generally increased with decreasing temperature. Regression of growth rate to second-stage (J2) and third-stage (J3) juveniles on temperature yielded different base temperatures: 6.3 degrees C and 4.4 degrees C for J2 and J3, respectively. Setting a base temperature of 6 degrees C allowed calculation of the degree-days (DD6) over which different life stages occurred. The largest population densities of J2 were found in roots between 50 and 200 DD6. Population densities of J3 peaked between 200 and 300 DD6. Adultmales were detected in soil starting at 300 to 400 DD6 and remained detectable for approximately 500 DD6. By 784 to 884 DD6, half of the eggs in adult females contained vermiform juveniles. Given the similarity in temperature ranges for successful development between G. ellingtonae and G. rostochiensis, G. ellingtonae populations likely could survive in the same geographic range in which G. rostochiensis now occurs. C1 [Phillips, Wendy S.; Kieran, Shannon Rose; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Phillips, WS (reprint author), USDA ARS, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM wendy.phillips@ars.usda.gov NR 27 TC 1 Z9 1 U1 3 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2015 VL 47 IS 4 BP 283 EP 289 PG 7 WC Zoology SC Zoology GA DF2HV UT WOS:000371164100001 PM 26941455 ER PT J AU Stetina, SR AF Stetina, Salliana R. TI Postinfection Development of Rotylenchulus reniformis on Resistant Gossypium barbadense Accessions SO JOURNAL OF NEMATOLOGY LA English DT Article DE cotton; Gossypium; reniform nematode; resistance; Rotylenchulus reniformis ID UPLAND COTTON RESISTANT; ROOT-KNOT NEMATODES; MELOIDOGYNE-ARENARIA RACE-1; GERMPLASM LINES; REGISTRATION; HISTOPATHOLOGY; HIRSUTUM; IDENTIFICATION; REPRODUCTION; PENETRATION AB The reniform nematode (Rotylenchulus reniformis) causes significant cotton (Gossypium hirsutum) losses in the southeastern United States. The research objective was to describe the effects of two resistant G. barbadense lines (cultivar TX 110 and accession GB 713) on development and fecundity of reniform nematode. Nematode development and fecundity were evaluated on the resistant lines and susceptible G. hirsutum cultivar Deltapine 16 in three repeated growth chamber experiments. Nematode development on roots early and late in the infection cycle was measured at set intervals from 1 to 25 d after inoculation (DAI) and genotypes were compared based on the number of nematodes in four developmental stages (vermiform, swelling, reniform, and gravid). At 15, 20, and 25 DAI, egg production by individual females parasitizing each genotype was measured. Unique reniform nematode developmental patterns were noted on each of the cotton genotypes. During the early stages of infection, infection and development occurred 1 d faster on susceptible cotton than on the resistant genotypes. Later, progression to the reniform and gravid stages of development occurred first on the susceptible genotype, followed by G. barbadense cultivar TX 110, and finally G. barbadense accession GB 713. Egg production by individual nematodes infecting the three genotypes was similar. This study corroborates delayed development previously reported on G. barbadense cultivar TX 110 and is the first report of delayed infection and development associated with G. barbadense accession GB 713. The different developmental patterns in the resistant genotypes suggest that unique or additional loci may confer resistance in these two lines. C1 [Stetina, Salliana R.] ARS, USDA, Crop Genet Res Unit, POB 345, Stoneville, MS 38776 USA. RP Stetina, SR (reprint author), ARS, USDA, Crop Genet Res Unit, POB 345, Stoneville, MS 38776 USA. EM Sally.Stetina@ars.usda.gov NR 41 TC 2 Z9 2 U1 1 U2 4 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2015 VL 47 IS 4 BP 302 EP 309 PG 8 WC Zoology SC Zoology GA DF2HV UT WOS:000371164100004 PM 26941458 ER PT J AU Morris, KA Langston, DB Dickson, DW Davis, RF Timper, P Noe, JP AF Morris, Kelly A. Langston, David B. Dickson, Donald W. Davis, Richard F. Timper, Patricia Noe, James P. TI Efficacy of Fluensulfone in a Tomato-Cucumber Double Cropping System SO JOURNAL OF NEMATOLOGY LA English DT Article DE cucumber; drip application; fluensulfone; management; Meloidogyne spp.; nematicide; root-knot nematodes; tomato ID ROOT-KNOT NEMATODE; MELOIDOGYNE-INCOGNITA; METHYL-BROMIDE; MANAGEMENT; RESISTANT; PEPPER; ALTERNATIVES; NEMATICIDE AB Vegetable crops in the southeastern United States are commonly grown on plastic mulch with two crop cycles produced on a single mulch application. Field trials were conducted in 2013 and 2014 in two locations to evaluate the efficacy of fluensulfone for controlling Meloidogyne spp. when applied through drip irrigation to cucumber in a tomato-cucumber double-cropping system. In the spring tomato crop, 1,3-dichloropropene (1,3-D), fluensulfone, and a resistant cultivar significantly decreased root galling by 91%, 73%, and 97%, respectively, compared to the untreated control. Tomato plots from the spring were divided into split plots for the fall where themain plots were the spring treatment and the subplots were cucumber either treated with fluensulfone (3.0 kg a.i./ha. via drip irrigation) or left untreated. The fall application of fluensulfone improved cucumber vigor and reduced gall ratings compared to untreated subplots. Fluensulfone reduced damage from root-knot nematodes when applied to the first crop as well as provided additional protection to the second crop when it was applied through a drip system. C1 [Morris, Kelly A.; Langston, David B.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Dickson, Donald W.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Davis, Richard F.; Timper, Patricia] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA USA. [Noe, James P.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. RP Morris, KA (reprint author), Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. EM dblangston@vt.edu NR 26 TC 2 Z9 2 U1 0 U2 0 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2015 VL 47 IS 4 BP 310 EP 315 PG 6 WC Zoology SC Zoology GA DF2HV UT WOS:000371164100005 PM 26941459 ER PT J AU Turgeon, JJ Orr, M Grant, C Wu, YK Gasman, B AF Turgeon, Jean J. Orr, Mary Grant, Cara Wu, Yunke Gasman, Ben TI DECADE-OLD SATELLITE INFESTATION OF ANOPLOPHORA GLABRIPENNIS MOTSCHULSKY (COLEOPTERA: CERAMBYCIDAE) FOUND IN ONTARIO, CANADA OUTSIDE REGULATED AREA OF FOUNDER POPULATION SO COLEOPTERISTS BULLETIN LA English DT Editorial Material ID ASIAN LONGHORNED BEETLE C1 [Turgeon, Jean J.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. [Orr, Mary; Grant, Cara] Canadian Food Inspect Agcy, Toronto, ON M3J 2E2, Canada. [Wu, Yunke] USDA, Otis Ctr Plant Hlth Sci Technol Lab, Joint Base Cape Cod, Buzzards Bay, MA 02542 USA. [Gasman, Ben] Canadian Food Inspect Agcy, Toronto, ON M3J 2E2, Canada. RP Turgeon, JJ (reprint author), Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM jean.turgeon@canada.ca; mary.orr@inspection.gc.ca; cara.grant@inspection.gc.ca; ben.gasman@inspection.gc.ca NR 12 TC 2 Z9 2 U1 0 U2 4 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD DEC PY 2015 VL 69 IS 4 BP 674 EP 678 DI 10.1649/0010-065X-69.4.674 PG 5 WC Entomology SC Entomology GA DE8OK UT WOS:000370895600010 ER PT J AU Hava, J Brown, R AF Hava, Jiri Brown, Robert TI A NEW RECORD OF MEGATOMA (PSEUDOHADROTOMA) GRAESERI (REITTER, 1887) (COLEOPTERA: DERMESTIDAE: MEGATOMINAE) FROM WISCONSIN, USA SO COLEOPTERISTS BULLETIN LA English DT Editorial Material C1 [Hava, Jiri] Czech Univ Life Sci, Fac Forestry & Wood Sci, Dept Forest Protect & Entomol, Kamycka 1176, CZ-16521 Prague 6, Suchdol, Czech Republic. [Brown, Robert] Purdue Univ, Dept Entomol, USDA APHIS PPQ, W Lafayette, IN 47907 USA. RP Hava, J (reprint author), Czech Univ Life Sci, Fac Forestry & Wood Sci, Dept Forest Protect & Entomol, Kamycka 1176, CZ-16521 Prague 6, Suchdol, Czech Republic. EM jh.dermestidae@volny.cz; Robert.C.Brown@aphis.usda.gov NR 3 TC 0 Z9 1 U1 1 U2 1 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD DEC PY 2015 VL 69 IS 4 BP 828 EP 829 DI 10.1649/0010-065X-69.4.828 PG 2 WC Entomology SC Entomology GA DE8OK UT WOS:000370895600034 ER PT J AU Chips, MJ Yerger, EH Hervanek, A Nuttle, T Royo, AA Pruitt, JN McGlynn, TP Riggall, CL Carson, WP AF Chips, Michael J. Yerger, Ellen H. Hervanek, Arpad Nuttle, Tim Royo, Alejandro A. Pruitt, Jonathan N. McGlynn, Terrence P. Riggall, Cynthia L. Carson, Walter P. TI The Indirect Impact of Long-Term Overbrowsing on Insects in the Allegheny National Forest Region of Pennsylvania SO NORTHEASTERN NATURALIST LA English DT Article ID WHITE-TAILED DEER; PLANT-COMMUNITIES; ARTHROPOD DIVERSITY; EXPERIMENTAL TESTS; VEGETATION; UNDERSTORY; ECOSYSTEM; DYNAMICS; LITTER; PSEUDOREPLICATION AB Overbrowsing has created depauperate plant communities throughout the eastern deciduous forest. We hypothesized these low-diversity plant communities are associated with lower insect diversity. We compared insects inside and outside a 60-year-old fenced deer exclosure where plant species richness is 5x higher inside versus outside. We sampled aboveground and litter insects using sweep nets and pitfall traps and identified specimens to family. Aboveground insect abundance, richness, and diversity were up to 50% higher inside the fenced exclosure versus outside. Conversely, litter insect abundance and diversity were consistently higher outside the exclosure. Community composition of aboveground insects differed throughout the summer (P < 0.05), but litter insects differed only in late summer. Our results demonstrate that the indirect effects of long-term overbrowsing can reduce aboveground insect diversity and abundance, and change composition even when plant communities are in close proximity. C1 [Chips, Michael J.; Hervanek, Arpad; Pruitt, Jonathan N.; Carson, Walter P.] Univ Pittsburgh, Dept Biol Sci, A234 Langley Hall, Pittsburgh, PA 15260 USA. [Yerger, Ellen H.; Nuttle, Tim] Indiana Univ Penn, Dept Biol, Indiana, PA 15705 USA. [Nuttle, Tim] Civil & Environm Consultants Inc, Ecol Serv Div, Pittsburgh, PA 15205 USA. [Royo, Alejandro A.] US Forest Serv, USDA, No Res Stn, Forestry Sci Lab, Irvine, PA 16329 USA. [McGlynn, Terrence P.; Riggall, Cynthia L.] Calif State Univ Dominguez Hills, Dept Biol, Carson, CA 90747 USA. RP Chips, MJ (reprint author), Univ Pittsburgh, Dept Biol Sci, A234 Langley Hall, Pittsburgh, PA 15260 USA. EM mjc119@pitt.edu FU Biology Department and the School of Graduate Studies and Research at the Indiana University of Pennsylvania FX Special thanks to R. Androw, R. Davidson, and J. Rawlins, of the Carnegie Museum of Natural History for aid in insect identification. N. Brouwer, A. Domic, E. Griffin, J. Hua, S.C. Pasquini, J. Slyder, and A. Stoler as well as 4 anonymous reviewers provided helpful comments on the manuscript. Thanks to J. Dzemyan and the Pennsylvania Game Commission for providing access to the study site, and S. Williamson and the 2011 Forest Ecology and Field Techniques in Ecology and Conservation classes at the University of Pittsburgh Pymatuning Laboratory of Ecology for help with data collection. We apppreciate the support of the Biology Department and the School of Graduate Studies and Research at the Indiana University of Pennsylvania. NR 57 TC 1 Z9 1 U1 5 U2 8 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1092-6194 EI 1938-5307 J9 NORTHEAST NAT JI Northeast. Nat PD DEC PY 2015 VL 22 IS 4 BP 782 EP 797 DI 10.1656/045.022.0412 PG 16 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8PP UT WOS:000370899000010 ER PT J AU Manee, C Rankin, WT Kauffman, G Adkison, G AF Manee, Christina Rankin, W. T. Duke Kauffman, Gary Adkison, Greg TI Association between Roads and the Distribution of Microstegium vimineum in Appalachian Forests of North Carolina SO SOUTHEASTERN NATURALIST LA English DT Article ID TRIN. A.-CAMUS; INVASIVE GRASS; JAPANESE STILTGRASS; HARDWOOD FOREST; SHADE-TOLERANT; VARIABLE LIGHT; C-4 GRASS; SPREAD; DIVERSITY; ENVIRONMENTS AB Microstegium vimineum (Japanese Stiltgrass) is an invasive, annual C4 grass that frequently forms dense populations along roads in the eastern US. We examined data from a survey that included 768 forested sites in western North Carolina, and carried out a transplant experiment to test (1) if the distribution of Japanese Stiltgrass is associated with roads and (2) if roadsides differ from forest interiors in terms of the frequency, abundance, and individual vigor of the species. Japanese Stiltgrass abundance was positively associated with total road length within watersheds. The species was much more common and abundant on roadsides than in forest interiors. Greenhouse-established individuals of Japanese Stiltgrass that we transplanted onto roadsides grew larger than those we transplanted in forest interiors. The 2 groups had similar survival rates. Our results suggest that roads promote the spread of Japanese Stiltgrass and that individuals and populations are more robust on roadsides than in forest interiors. However, the species can grow in forest interiors, suggesting its lower abundance and size there may result from limitations in dispersal, germination, or resource acquisition. C1 [Manee, Christina; Adkison, Greg] Western Carolina Univ, Dept Biol, Cullowhee, NC 28723 USA. [Manee, Christina] Asheville Buncombe Tech Community Coll, Dept Biol, Asheville, NC 28801 USA. [Rankin, W. T. Duke] US Forest Serv, USDA, Atlanta, GA 30309 USA. [Kauffman, Gary] US Forest Serv, USDA, Asheville, NC 28801 USA. RP Adkison, G (reprint author), Western Carolina Univ, Dept Biol, Cullowhee, NC 28723 USA. EM gadkison@wcu.edu NR 26 TC 0 Z9 1 U1 4 U2 7 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD DEC PY 2015 VL 14 IS 4 BP 602 EP 611 DI 10.1656/058.014.0402 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NL UT WOS:000370892600001 ER PT J AU Cameron, RS Hanula, J Fraedrich, S Bates, C AF Cameron, R. Scott Hanula, James Fraedrich, Stephen Bates, Chip TI Progression and Impact of Laurel Wilt Disease within Redbay and Sassafras Populations in Southeast Georgia SO SOUTHEASTERN NATURALIST LA English DT Article ID AMBROSIA BEETLE COLEOPTERA; XYLEBORUS-GLABRATUS COLEOPTERA; CURCULIONIDAE SCOLYTINAE; RAFFAELEA-LAURICOLA; 1ST REPORT; UNITED-STATES; INVASION; FLORIDA; TREES; DIAMETER AB Laurel wilt disease (LWD), caused by the fungus Raffaelea lauricola and transmitted by Xyleborus glabratus (Redbay Ambrosia Beetle [RAB]), has killed millions of Persea borbonia (Redbay) trees throughout the southeastern Coastal Plain. Laurel wilt also has been detected in Sassafras albidum (Sassafras) in widely dispersed locations across the southeastern US. We established long-term laurel wilt disease-progression plots in Redbay and Sassafras stands in southeastern Georgia and monitored them through 4 years to document mortality rates and investigate long-term effects of LWD on Redbay and Sassafras survival and regeneration. Laurel wilt disease killed 87.3% of Redbay and 79.5% of Sassafras trees in the plots. The time from initial LWD detection to inactivity (no new mortality) in Redbay stands ranged from 1.1 to 3.6 years, with rate of disease progression positively related to host-tree size and abundance. Larger trees died at a higher rate in both Redbay and Sassafras stands, and mortality curves were similar for both species. All diseased Redbay trees died to the ground level, but the majority produced persistent below-ground basal sprouts, rapidly providing potential replacement stems. Few below-ground basal sprouts were observed on Sassafras trees killed by LWD, but over a quarter had epicormic shoots that survived up to several years after infection, and small trees remained alive on most sites, suggesting some level of tolerance to LWD. Substantial numbers of RAB were only captured in baited traps located adjacent to plots in an advanced-active stage of disease progression with abundant infested trees, both in Redbay and Sassafras stands. However, lingering presence of small numbers of RAB in post-epidemic areas and scattered LWD mortality in small-sized Redbay regeneration sprouts and seedlings suggest that secondary disease cycles may occur as Redbay trees there reach greater numbers and size in the future. Documentation of RAB and LWD spreading in Sassafras in the absence of Redbay supports concern that LWD will continue to spread into areas with abundant, large Sassafras trees, which would increase the probability that RAB and LWD will expand into extensive populations of other laurel species present in the western US and Central and South America. C1 [Cameron, R. Scott; Bates, Chip] Georgia Forestry Commiss, Statesboro, GA 30641 USA. [Hanula, James; Fraedrich, Stephen] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Hanula, J (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM jim.hanula@gmail.com FU USDA Forest Service, Forest Health Protection, Southern Region; Georgia Forestry Commission FX Funding for this Evaluation Monitoring (EM) Project was provided by the USDA Forest Service, Forest Health Protection, Southern Region and the Georgia Forestry Commission. We thank Dr. Joel Gramling (The Citadel) for help with plot design and sampling protocol, and Susan Best and Mike Cody for assistance in the laboratory. NR 35 TC 3 Z9 3 U1 2 U2 5 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD DEC PY 2015 VL 14 IS 4 BP 650 EP 674 DI 10.1656/058.014.0408 PG 25 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NL UT WOS:000370892600006 ER PT J AU Bragg, DC AF Bragg, Don C. TI Clarifying Details on a 1930s-era Pine-Hardwood Stand in Arkansas SO SOUTHEASTERN NATURALIST LA English DT Article AB Data from recently discovered daily-work logs of US Forest Service (USFS) researcher Russell R. Reynolds enabled me to clarify a study I published a decade ago on a 1930s-vintage unmanaged, second-growth Pinus (pine)-hardwood stand in southeastern Arkansas. Though still too vague to reveal every detail, Reynolds' work logs confirmed a number of assumptions in the original paper and provided me with the background information to herein describe a more precise sampling framework for this 1930s-era study plot. C1 [Bragg, Don C.] Univ Arkansas Monticello, US Forest Serv, USDA, Southern Res Stn, POB 3516, Monticello, AR 71656 USA. RP Bragg, DC (reprint author), Univ Arkansas Monticello, US Forest Serv, USDA, Southern Res Stn, POB 3516, Monticello, AR 71656 USA. EM dbragg@fs.fed.us FU US Forest Service's Southern Research Station FX First, I would like to recognize the foresight of Russ Reynolds to record his activities many decades ago, which made this paper and the previous one possible. I am also grateful to the following for their assistance on this effort: O.H. "Doogie" Darling, Mike Shelton, Jim Guldin, and 2 anonymous reviewers. This work was supported by the US Forest Service's Southern Research Station. NR 5 TC 0 Z9 0 U1 1 U2 1 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD DEC PY 2015 VL 14 IS 4 BP N50 EP N54 DI 10.1656/058.014.0410 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NL UT WOS:000370892600016 ER PT J AU Centi, AJ Booth, SL Gundberg, CM Saltzman, E Nicklas, B Shea, MK AF Centi, Amanda J. Booth, Sarah L. Gundberg, Caren M. Saltzman, Edward Nicklas, Barbara Shea, M. Kyla TI Osteocalcin carboxylation is not associated with body weight or percent fat changes during weight loss in post-menopausal women SO ENDOCRINE LA English DT Article DE Osteocalcin; Vitamin K; Weight loss; Body fat ID VITAMIN-K STATUS; UNDERCARBOXYLATED OSTEOCALCIN; SERUM OSTEOCALCIN; ENERGY-METABOLISM; BONE TURNOVER; GLUCOSE-HOMEOSTASIS; INSULIN-RESISTANCE; PLASMA OSTEOCALCIN; PHYLLOQUINONE; EXERCISE AB Osteocalcin (OC) is a vitamin K-dependent bone protein used as a marker of bone formation. Mouse models have demonstrated a role for the uncarboxylated form of OC (ucOC) in energy metabolism, including energy expenditure and adiposity, but human data are equivocal. The purpose of this study was to determine the associations between changes in measures of OC and changes in body weight and percent body fat in obese, but otherwise healthy post-menopausal women undergoing a 20-week weight loss program. All participants received supplemental vitamins K and D and calcium. Body weight and body fat percentage (% BF) were assessed before and after the intervention. Serum OC [(total (tOC), ucOC, percent uncarboxylated (% ucOC)], and procollagen type 1N-terminal propeptide (P1NP; a measure of bone formation) were measured. Women lost an average of 10.9 +/- 3.9 kg and 4 % BF. Serum concentrations of tOC, ucOC, % ucOC, and P1NP did not significantly change over the twenty-week intervention, nor were these measures associated with changes in weight (all p > 0.27) or % BF (all p > 0.54). Our data do not support an association between any serum measure of OC and weight or % BF loss in post-menopausal women supplemented with nutrients implicated in bone health. C1 [Centi, Amanda J.; Booth, Sarah L.; Saltzman, Edward; Shea, M. Kyla] Tufts Univ, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Gundberg, Caren M.] Yale Univ, Sch Med, New Haven, CT USA. [Nicklas, Barbara] Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC USA. RP Booth, SL (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM sarah.booth@tufts.edu FU NIH/NIDDK [R01AGDK20583]; Wake Forest University Claude D. Pepper Older Americans Independence Center [P30-AG21332]; Wake Forest University General Clinical Research Center [M01-RR07122]; USDA Agricultural Research Service [58-1950-7-707] FX Supported by NIH/NIDDK Grant No. R01AGDK20583, Wake Forest University Claude D. Pepper Older Americans Independence Center (P30-AG21332), Wake Forest University General Clinical Research Center (M01-RR07122) and USDA Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707. NR 51 TC 1 Z9 1 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1355-008X EI 1559-0100 J9 ENDOCRINE JI Endocrine PD DEC PY 2015 VL 50 IS 3 BP 627 EP 632 DI 10.1007/s12020-015-0618-6 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DD7CX UT WOS:000370082400013 PM 25963022 ER PT J AU Bauermann, FV Falkenberg, SM Decaro, N Flores, EF Ridpath, JF AF Bauermann, F. V. Falkenberg, S. M. Decaro, N. Flores, E. F. Ridpath, J. F. TI Experimental infection of calves, sheep, goats and pigs with HoBi-like viruses by direct inoculation or exposure to persistently infected calves SO VETERINARY MICROBIOLOGY LA English DT Article DE Bovine viral diarrhea virus; Clinical signs; Epidemiology; Pestivirus; Transmission ID VIRAL DIARRHEA VIRUS; PESTIVIRUS; CATTLE; SERUM AB HoBi-like viruses are an emerging species of pestiviruses associated with respiratory and reproductive disease in cattle and in water buffaloes. Although cattle appear to be the main natural hosts, little is know about the potential for HoBi-like viruses to be transmitted to other livestock. In this study, seronegative calves, goats and pigs, and sheep harboring pestivirus antibodies (probably due to previous exposure to BVDV) were exposed to HoBi-like viruses either by direct inoculation (Gin) or by contact with calves persistently infected with HoBi-like viruses (GEx). Both Gin and GEx groups were monitored for clinical signs, lymphocyte count, virus in buffy coats and nasal swabs up to day 18 post-inoculation (pi). Evidence of transmission of HoBi-like virus by PI calves was observed in all studied species. No difference in clinical presentation was observed between animals in the Gin or GEx groups. Evidence of infection, depending on the species included lymphocyte depletion, fever, viral RNA detection, and/or seroconversion. Depletion of lymphocytes was observed in calves and goats (35% and 50%, respectively) but not in pigs. Seroconversion was observed in at least one animal of each group and for all exposed species. The rate of seroconversion was higher in animals in the GIn experimental groups. In sheep, pre-existing moderate to high neutralizing titers against BVDV did not prevent viral replication and shed. The study demonstrated that naive cattle, goats and pigs, in addition to antibody positive sheep, can be infected by HoBi-like virus via persistently infected calf and potentially transmit the virus. Published by Elsevier B.V. C1 [Bauermann, F. V.; Ridpath, J. F.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Bauermann, F. V.; Flores, E. F.] Univ Fed Santa Maria, BR-97015040 Santa Maria, RS, Brazil. [Falkenberg, S. M.] Elanco Anim Hlth, Vaccine Dev, Greenfield, IN 46160 USA. [Decaro, N.] Univ Bari, Dept Vet Med, Valenzano, Italy. RP Bauermann, FV (reprint author), ARS, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM Fernando.bauermann@ars.usda.gov RI flores, eduardo/H-5125-2016; OI Decaro, Nicola/0000-0001-6348-7969 NR 13 TC 3 Z9 3 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD DEC PY 2015 VL 181 IS 3-4 BP 289 EP 293 DI 10.1016/j.vetmic.2015.10.011 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA DE2MD UT WOS:000370460500014 PM 26525738 ER PT J AU Dinarti, D Susilo, AW Meinhardt, LW Ji, K Motilal, LA Mischke, S Zhang, DP AF Dinarti, Diny Susilo, Agung W. Meinhardt, Lyndel W. Ji, Kun Motilal, Lambert A. Mischke, Sue Zhang, Dapeng TI Genetic diversity and parentage in farmer selections of cacao from Southern Sulawesi, Indonesia revealed by microsatellite markers SO BREEDING SCIENCE LA English DT Article DE chocolate; molecular markers; participatory breeding; rehabilitation; Southeast Asia; tropical plant ID THEOBROMA-CACAO; COCOA; INFERENCE; SOFTWARE AB Indonesia is the third largest cocoa-producing country in the world. Knowledge of genetic diversity and parentage of farmer selections is important for effective selection and rational deployment of superior cacao clones in farmers' fields. We assessed genetic diversity and parentage of 53 farmer selections of cacao in Sulawesi, Indonesia, using 152 international clones as references. Cluster analysis, based on 15 microsatellite markers, showed that these Sulawesi farmer selections are mainly comprised of hybrids derived from Trinitario and two Upper Amazon Forastero groups. Bayesian assignment and likelihood-based parentage analysis further demonstrated that only a small number of germplasm groups, dominantly Trinitario and Parinari, contributed to these fanner selections, in spite of diverse parental clones having been used in the breeding program and seed gardens in Indonesia since the 1950s. The narrow parentage predicts a less durable host resistance to cacao diseases. Limited access of the farmers to diverse planting materials or the strong preference for large pods and large bean size by local farmers, may have affected the selection outcome. Diverse sources of resistance, harbored in different cacao germplasm groups, need to be effectively incorporated to broaden the on-farm diversity and ensure sustainable cacao production in Sulawesi. C1 [Dinarti, Diny] Bogor Agr Univ, Fac Agr, PMBL, Dept Agron & Hort, Bogor 16680, Indonesia. [Susilo, Agung W.] ICCRI, Jember 68118, Indonesia. [Meinhardt, Lyndel W.; Ji, Kun; Mischke, Sue; Zhang, Dapeng] USDA ARS, Sustainable Perennial Crops Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Ji, Kun] Southwest Univ, Coll Hort & Landscape, Chongqing 400715, Peoples R China. [Motilal, Lambert A.] Univ W Indies, Cocoa Res Ctr, St Augustine, Trinidad, Trinid & Tobago. RP Zhang, DP (reprint author), USDA ARS, Sustainable Perennial Crops Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Dapeng.Zhang@ars.usda.gov FU USDA-FAS; World Cocoa Foundation FX The authors would like to thank Stephen Pinney and Yan Mei-Li of USDA-ARS, SPCL for assistance in DNA sample preparation and SSR genotyping, and Virginia Sopyla of the World Cocoa Foundation for logistical support. The authors would also like to acknowledge USDA-FAS and the World Cocoa Foundation for the Borlaug Cocoa Fellowship program. NR 43 TC 0 Z9 0 U1 1 U2 5 PU JAPANESE SOC BREEDING PI TOKYO PA UNIV TOKYO, GRADUATE SCHOOL AGRICULTURAL LIFE SCIENCES, BUNKYO-KU, TOKYO, 113-8657, JAPAN SN 1344-7610 EI 1347-3735 J9 BREEDING SCI JI Breed. Sci. PD DEC PY 2015 VL 65 IS 5 BP 438 EP 446 DI 10.1270/jsbbs.65.438 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DD2TU UT WOS:000369775900010 PM 26719747 ER PT J AU Arimond, M Zeilani, M Jungjohann, S Brown, KH Ashorn, P Allen, LH Dewey, KG AF Arimond, Mary Zeilani, Mamane Jungjohann, Svenja Brown, Kenneth H. Ashorn, Per Allen, Lindsay H. Dewey, Kathryn G. TI Considerations in developing lipid-based nutrient supplements for prevention of undernutrition: experience from the International Lipid-Based Nutrient Supplements (iLiNS) Project SO MATERNAL AND CHILD NUTRITION LA English DT Article DE home fortification; undernutrition; prevention; infant; young child; pregnancy; lactation; micronutrients; essential fatty acids; Africa ID LOW-INCOME COUNTRIES; USE THERAPEUTIC FOOD; MODERATELY MALNOURISHED CHILDREN; DENSE COMPLEMENTARY FOOD; BREAST-MILK INTAKE; FATTY-ACID STATUS; CORN-SOY BLEND; TO-USE FOOD; MALAWIAN CHILDREN; YOUNG-CHILDREN AB The International Lipid-Based Nutrient Supplements (iLiNS) Project began in 2009 with the goal of contributing to the evidence base regarding the potential of lipid-based nutrient supplements (LNS) to prevent undernutrition in vulnerable populations. The first project objective was the development of acceptable LNS products for infants 6-24 months and for pregnant and lactating women, for use in studies in three countries (Burkina Faso, Ghana and Malawi). This paper shares the rationale for a series of decisions in supplement formulation and design, including those related to ration size, ingredients, nutrient content, safety and quality, and packaging. Most iLiNS supplements have a daily ration size of 20 g and are intended for home fortification of local diets. For infants, this ration size is designed to avoid displacement of breast milk and to allow for dietary diversity including any locally available and accessible nutrient-dense foods. Selection of ingredients depends on acceptability of flavour, micronutrient, anti-nutrient and essential fatty acid contents. The nutrient content of LNS designed to prevent undernutrition reflects the likelihood that in many resource-poor settings, diets of the most nutritionally vulnerable individuals (infants, young children, and pregnant and lactating women) are likely to be deficient in multiple micronutrients and, possibly, in essential fatty acids. During ingredient procurement and LNS production, safety and quality control procedures are required to prevent contamination with toxins or pathogens and to ensure that the product remains stable and palatable over time. Packaging design decisions must include consideration of product protection, stability, convenience and portion control. C1 [Arimond, Mary; Brown, Kenneth H.; Allen, Lindsay H.; Dewey, Kathryn G.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Arimond, Mary; Brown, Kenneth H.; Allen, Lindsay H.; Dewey, Kathryn G.] Univ Calif Davis, Program Int & Community Nutr, Davis, CA 95616 USA. [Zeilani, Mamane] Nutriset SAS, Malaunay, France. [Jungjohann, Svenja] Global Alliance Improved Nutr, Geneva, Switzerland. [Ashorn, Per] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland. [Allen, Lindsay H.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. Care of Dewey K, Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Arimond, M (reprint author), Care of Dewey K, Univ Calif Davis, Dept Nutr, One Shields Ave, Davis, CA 95616 USA. EM marimond@ucdavis.edu FU Bill & Melinda Gates Foundation FX This publication is based on research funded in part by the Bill & Melinda Gates Foundation. The findings and conclusions contained within are those of the authors and do not necessarily reflect positions or policies of the Bill & Melinda Gates Foundation. Nutriset partially covered the cost of product development and provided products for the acceptability trials. NR 103 TC 22 Z9 22 U1 4 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1740-8695 EI 1740-8709 J9 MATERN CHILD NUTR JI Matern. Child Nutr. PD DEC PY 2015 VL 11 SU 4 BP 31 EP 61 DI 10.1111/mcn.12049 PG 31 WC Nutrition & Dietetics; Pediatrics SC Nutrition & Dietetics; Pediatrics GA DD5DO UT WOS:000369943000004 PM 23647784 ER PT J AU Will-Wolf, S Makholm, MM Nelsen, MP Trest, MT Reis, AH Jovan, S AF Will-Wolf, Susan Makholm, Martha M. Nelsen, Matthew P. Trest, Marie T. Reis, Anne H. Jovan, Sarah TI Element analysis of two common macrolichens supports bioindication of air pollution and lichen response in rural midwestern USA SO BRYOLOGIST LA English DT Article DE Air quality; bioindicator; eastern USA; Flavoparmelia caperata; forest cover; air pollution; Punctelia rudecta ID WESTERN UNITED-STATES; EPIPHYTIC LICHENS; PARMELIA-SULCATA; FLAVOPARMELIA-CAPERATA; ATMOSPHERIC POLLUTANTS; AIRBORNE POLLUTANTS; NITROGEN DEPOSITION; LANDSCAPE PATTERN; CRITICAL LOADS; HEAVY-METALS AB Element analysis was conducted on naturally-growing Flavoparmelia caperata (L.) Hale and Punctelia rudecta (Ach.) Krog (26 sites) in 2003-05 for a 30-year resurvey of forest lichen communities near a power plant in Wisconsin. Cu, Cr, N, and S increased strongly with power plant impact (66 samples, both species) and are good candidate bioindicators for local pollution; Al and Fe increased weakly and are not recommended. Hg is a candidate pollution indicator from comparison with a background site (only F. caperata data: 39 samples, 23 sites). Only N and S were correlated with lichen species abundance and are thus candidate bioindicators for lichen response. Abundance of P. rudecta was lower and that of Phaeophyscia pusilloides (Zahlbr.) Essl. was higher with more N or S in lichens; abundances of F. caperata and large foliose species as a group were lower with higher modeled SO2 from the power plant (no response from four other tested lichen species or groups). Sites in more forested landscapes to the west of the power plant had more lichen species, including disturbance-sensitive taxa. Heathier lichens there may have led to higher concentrations of Al, Cr, and S, plus Li (only F. caperata data). Univariate general linear modeling (GLM) was more useful than regression to test species effect at 7 sites. Element analysis confirmed earlier records of minimal power plant impact on lichens in this area, where no truly pollution-sensitive lichen species have been recorded for decades. C1 [Will-Wolf, Susan; Trest, Marie T.] Univ Wisconsin, Dept Bot, 430 Lincoln Dr, Madison, WI 53706 USA. [Makholm, Martha M.] Bur Air Management, Wisconsin Dept Nat Resources, Madison, WI 53707 USA. [Nelsen, Matthew P.] Stanford Univ, Stanford, CA 94305 USA. [Reis, Anne H.] Urban Ecol Ctr, Milwaukee, WI 53211 USA. [Jovan, Sarah] US Forest Serv, USDA, Portland Forestry Sci Lab, Portland, OR 97205 USA. RP Will-Wolf, S (reprint author), Univ Wisconsin, Dept Bot, 430 Lincoln Dr, Madison, WI 53706 USA. EM swwolf@wisc.edu FU State of Wisconsin, Focus on Energy Environmental Research Program [FY02-03] FX This project was funded by the State of Wisconsin, Focus on Energy Environmental Research Program, Research Agreement Grant #FY02-03 to University of Wisconsin-Madison, U.S.A., for research by Susan Will-Wolf. Students in Professor Brian Yandell's Spring 2007 University of Wisconsin-Madison Statistics 998 (Statistical Consulting) seminar pointed out the importance of year effect for some elements and made other suggestions that helped improve our analyses. James Bennett provided lichen element data from several U.S. National Park Service internal reports. We thank Emily Holt and two anonymous reviewers for critical comments that helped us improve the manuscript. NR 60 TC 0 Z9 0 U1 11 U2 27 PU AMER BRYOLOGICAL LICHENOLOGICAL SOC INC PI OMAHA PA C/O DR ROBERT S EGAN, SEC-TRES, ABLS, UNIV NEBRASKA OMAHA, DEPT BIOLOGY, OMAHA, NE 68182-0040 USA SN 0007-2745 EI 1938-4378 J9 BRYOLOGIST JI Bryologist PD WIN PY 2015 VL 118 IS 4 BP 371 EP 384 DI 10.1639/0007-2745-118.4.371 PG 14 WC Plant Sciences SC Plant Sciences GA DD2TV UT WOS:000369776000003 ER PT J AU Sachdeva, S Jordan, J Mazar, N AF Sachdeva, Sonya Jordan, Jennifer Mazar, Nina TI Green consumerism: moral motivations to a sustainable future SO CURRENT OPINION IN PSYCHOLOGY LA English DT Article ID CONSPICUOUS CONSERVATION; ENVIRONMENTAL BEHAVIOR; CLIMATE-CHANGE; SOCIAL NORMS; CONSUMPTION; PRODUCTS; ENERGY; WORLD; NUDGES; HOPE AB Green consumerism embodies a dilemma inherent in many prosocial and moral actions -foregoing personal gain in favor of a more abstract, somewhat intangible gain to someone or something else. In addition, as in the case of purchasing more expensive green products, there is sometimes a very literal cost that may act as a barrier to engaging in green consumerism. The current review examines endogenous, exogenous, and structural factors that promote green consumerism. We also discuss its potential positive and negative spillover effects. We close by discussing areas of research on green consumerism that are lacking -such as the moral framing of green consumerism and the expansion of the cultural context in which it is defined and studied. C1 [Sachdeva, Sonya] US Forest Serv, Evanston, IL 60201 USA. [Jordan, Jennifer] Univ Groningen, NL-9700 AB Groningen, Netherlands. [Mazar, Nina] Univ Toronto, Toronto, ON M5S 1A1, Canada. RP Sachdeva, S (reprint author), US Forest Serv, Evanston, IL 60201 USA. EM sonyasachdeva@fs.fed.us RI Mazar, Nina/B-7697-2011 OI Mazar, Nina/0000-0001-8248-654X NR 63 TC 2 Z9 2 U1 24 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2352-250X J9 CURR OPIN PSYCHOL JI Curr. Opin. Psychol. PD DEC PY 2015 VL 6 BP 60 EP 65 DI 10.1016/j.copsyc.2015.03.029 PG 6 WC Psychology, Multidisciplinary SC Psychology GA DC9XW UT WOS:000369575900014 ER PT J AU Cornelius, ML Gallatin, EM AF Cornelius, Mary L. Gallatin, Erin M. TI Task allocation in the tunneling behavior of workers of the Formosan subterranean termite, Coptoterrnes formosanus Shiraki SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Tunnel; Excavation; Gallery system; Polyethism ID DIVISION-OF-LABOR; ISOPTERA RHINOTERMITIDAE; RETICULITERMES FUKIENENSIS; SIZE; FOOD; COLONIES AB This study provided a detailed analysis of the tunneling behavior of 30 marked individuals by examining the role of each individual in tunnel excavation on three consecutive days. The objective of this study was to identify the specific individuals that were the most active in the initiation of a new tunnel on the first day in order to determine if these specific individuals continued to play a key role in tunnel excavation as the tunnel expanded on the second day. In addition, this study examined the behavior of the same group of individuals in the initiation of a new tunnel on the third day. Overall, there was an average of three individuals transporting >20 loads of sand, 13 individuals transporting between 1 and 20 loads, and 14 individuals that did not transport any loads during each 2-h observation period. Top excavators during tunnel initiation were significantly more likely to continue to be top excavators during tunnel expansion than other individuals. These top excavators were also more likely to perform the most work in the excavation of a new tunnel on the third day. These results demonstrate that specific individuals remain highly active for at least 3 days which provides further evidence that top excavators act as organizers in determining the orientation and branching patterns in the tunneling networks of C. formosanus. Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. C1 [Cornelius, Mary L.; Gallatin, Erin M.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Cornelius, ML (reprint author), ARS, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Mary.Cornelius@ars.usda.gov NR 20 TC 0 Z9 0 U1 387 U2 388 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD DEC PY 2015 VL 18 IS 4 BP 637 EP 642 DI 10.1016/j.aspen.2015.07.017 PG 6 WC Entomology SC Entomology GA DC6SJ UT WOS:000369349600001 ER PT J AU Folta, SC Seguin, RA Chui, KKH Clark, V Corbin, MA Goldberg, JP Heidkamp-Young, E Lichtenstein, AH Wiker, N Nelson, ME AF Folta, Sara C. Seguin, Rebecca A. Chui, Kenneth K. H. Clark, Valerie Corbin, Marilyn A. Goldberg, Jeanne P. Heidkamp-Young, Eleanor Lichtenstein, Alice H. Wiker, Nancy Nelson, Miriam E. TI National Dissemination of Strong Women-Healthy Hearts: A Community-Based Program to Reduce Risk of Cardiovascular Disease Among Midlife and Older Women SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID PHYSICAL-ACTIVITY QUESTIONNAIRE; AFRICAN-AMERICAN WOMEN; LIFE-STYLE; INTERVENTION; PROMOTION; IMPROVE; ADULTS; DIET; ASSOCIATION; PREVENTION AB Objectives. We describe the national dissemination of an evidence-based community cardiovascular disease prevention program for midlife and older women using the RE-AIM (reach effectiveness adoption implementation maintenance) framework and share key lessons learned during translation. Methods. In a 2010 to 2014 collaboration between the Strong Women program and the National Extension Association of Family and Consumer Sciences, we assessed reach, adoption, implementation, and maintenance using survey methods, and we assessed effectiveness using a pretest-posttest within-participants design, with weight change as the primary outcome. Results. Overall reach into the population was 15 per 10 000. Of 85 trained leaders, 41 (48%) adopted the program. During the 12-week intervention, weight decreased by 0.5 kilograms, fruit and vegetable intake increased by 2.1 servings per day, and physical activity increased by 1238 metabolic equivalent (MET)minutes per week (all P < .001). Average fidelity score was 4.7 (out of possible 5). Eleven of 41 adopting leaders (27%) maintained the program. Conclusions. The Strong Women-Healthy Hearts program can be implemented with high fidelity in a variety of settings while remaining effective. These data provide direction for program modification to improve impact as dissemination continues. C1 [Folta, Sara C.; Clark, Valerie; Goldberg, Jeanne P.; Heidkamp-Young, Eleanor; Nelson, Miriam E.] Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Boston, MA 02111 USA. [Chui, Kenneth K. H.] Tufts Univ, Dept Publ Hlth & Community Med, Sch Med, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Seguin, Rebecca A.] Cornell Univ, Div Nutr Sci, Ithaca, NY USA. [Corbin, Marilyn A.] Penn State Extens, University Pk, PA USA. [Wiker, Nancy] Penn State Cooperat Extens, Lancaster, PA USA. RP Folta, SC (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Boston, MA 02111 USA. EM sara.folta@tufts.edu FU Centers for Disease Control and Prevention, Atlanta, GA [1R18DP002144] FX This study was funded by supported by Cooperative Agreement No. 1R18DP002144 from the Centers for Disease Control and Prevention, Atlanta, GA. NR 42 TC 2 Z9 2 U1 2 U2 3 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD DEC PY 2015 VL 105 IS 12 BP 2578 EP 2585 DI 10.2105/AJPH.2015.302866 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DC4BB UT WOS:000369163600043 PM 26469644 ER PT J AU Saenz-Romero, C Rehfeldt, GE Ortega-Rodriguez, JM Marin-Togo, MC Madrigal-Sanchez, X AF Saenz-Romero, Cuauhtemoc Rehfeldt, Gerald E. Manuel Ortega-Rodriguez, Juan Consuelo Marin-Togo, Maria Madrigal-Sanchez, Xavier TI PINUS LEIOPHYLLA SUITABLE HABITAT FOR 1961-1990 AND FUTURE CLIMATE SO BOTANICAL SCIENCES LA English DT Article DE assisted migration; climate change impacts; Random Forests classification tree; responses to climate ID GENETIC-VARIATION; UNITED-STATES; GLOBAL-CHANGE; FORESTS; POPULATIONS; SCENARIOS; IMPACTS; MEXICO; RESPONSES; MODEL AB Our objectives were to predict and map the climatic niche for Pinus leiophylla for a period of normalization (years 1961-1990) and future (2030, 2060 and 2090) climates, and to suggest management strategies to accommodate climate changes, and discuss implications for conservation. A bioclimate model predicting the presence or absence of P. leiophylla (lumped with its putative variety P. leiophylla var. chihuahuana) was developed by using the Random Forests classification tree on Mexican and Unites States of America forest inventory data. The bioclimatic model had an average error of prediction of 4.6 %. The model used six predictor variables, dominated by precipitation variables. Projecting the 1961-1990 climate niche into future climates provided by three general circulation models and two greenhouse-effect gas emission scenarios, suggested that the area occupied by the niche should diminish rapidly over the course of the century: a decrease of 35 % by the decade surrounding 2030, 50 % for 2060, and 76 % for 2090. The most serious habitat reduction occurs at both latitudinal extremes of the species distribution: Chiricagua Mountains, Arizona, Unites States of America in the northern extreme, and at Oaxaca State, Mexico, in the southernmost extreme. There is no indication at all of expansion of suitable climatic habitat northwards. We urge establishing seed banks encompassing seed from provenances sampled from the largest part possible of the natural distribution, and start assisted migration tests, to realign the natural populations with the climate for which they are adapted and that will occur at higher altitudes. C1 [Saenz-Romero, Cuauhtemoc] Univ Michoacana, Inst Invest Agr & Forestales, Morelia, Michoacan, Mexico. [Rehfeldt, Gerald E.] US Forest Serv, Forestry Sci Lab, Mt Res Stn, USDA, Moscow, ID USA. [Manuel Ortega-Rodriguez, Juan; Consuelo Marin-Togo, Maria; Madrigal-Sanchez, Xavier] Univ Michoacana, Fac Biol, Morelia, Michoacan, Mexico. RP Saenz-Romero, C (reprint author), Univ Michoacana, Inst Invest Agr & Forestales, Morelia, Michoacan, Mexico. EM csaenzromero@gmail.com FU Mexican Commission for the Study and Use of the Biodiversity (CONABIO) [JM036]; Coordination for Scientific Research of the University of Michoacan (CIC, UMSNH); Mexican Integral Program for Institutional Strengthening Fund [PIFI-2012] FX Financial support to CSR was provided by a grant from Mexican Commission for the Study and Use of the Biodiversity (CONABIO, project JM036), the Coordination for Scientific Research of the University of Michoacan (CIC, UMSNH), and the Mexican Integral Program for Institutional Strengthening Fund (PIFI-2012). We thank Miriam Vargas-Llamas, Rigoberto Palafox-Rivas, and Octavio Magalia-Torres, Mexican National Forestry Commission (CONAFOR) for providing Mexican forest inventory data; Nicholas Crookston (USDA-Forest Service, Moscow, Idaho, for technical support; Jorge Alberto Perez-de-la Rosa (Universidad de Guadalajara), Rebeca Aldana-Barajas (CONAFOR), Celestino Flores-Lopez (Universidad Autonoma Agraria Antonio Narro), Christian Wehenkel (Universidad Juarez del Estado de Durango), Socorro Gonzales-Elizondo (CIIDIR-Durango, Instituto Politecnico Nacional), Javier Lopez-Upton (Colegio de Postgraduados) and Antonio Plancarte-Barrera (Alen Mexico), for valuable comments about quality of data input, taxonomic status and preliminary maps. Two anonymous reviewers made valuable comments that helped to improve the manuscript. NR 49 TC 3 Z9 3 U1 1 U2 8 PU SOC BOTANICA MEXICO PI MEXICO PA APARTADO POSTAL 70-385 DELEGACION COYOACAN, CIUDAD UNIV, MEXICO, D F 00000, MEXICO SN 2007-4298 EI 2007-4476 J9 BOT SCI JI Bot. Sci. PD DEC PY 2015 VL 93 IS 4 BP 709 EP 718 PG 10 WC Plant Sciences SC Plant Sciences GA DC2KZ UT WOS:000369046900003 ER PT J AU DuPraw, ME Bedell-Loucks, A Enzer, MJ Lyon, KM Silvas, D Thorpe, LA Williams, PB AF DuPraw, Marcelle E. Bedell-Loucks, Andrea Enzer, Maia J. Lyon, Katie M. Silvas, Daniel Thorpe, Laurie A. Williams, Peter B. TI From Personality-Driven to Institutionally Driven Collaboration by the US Forest Service SO CONFLICT RESOLUTION QUARTERLY LA English DT Article ID MANAGEMENT; FRAMEWORK; CAPACITY AB This article describes an approach being used by the US Department of Agriculture's Forest Service to assess and strengthen agency-wide capacity to collaborate effectively with external stakeholders, as well as strategic reasons for investing resources in this endeavor in an era of fiscal austerity. The article examines the role of the third-party neutral in helping agency stakeholders chart the path forward, in the near term and the longer term, and offers reflections on the characteristics of that role thataremost important to the efficacy of the over-all effort in this particular case. C1 [DuPraw, Marcelle E.] Calif State Univ Sacramento, Ctr Collaborat Policy, Sacramento, CA 95819 USA. [DuPraw, Marcelle E.; Bedell-Loucks, Andrea] US Forest Serv, Cooperat Forestry, Washington, DC USA. [Enzer, Maia J.] US Forest Serv, Pacific Northwest Reg, Washington, DC USA. [Lyon, Katie M.] Colorado State Univ, Human Dimens Nat Resources, Ft Collins, CO 80523 USA. [Silvas, Daniel] US Forest Serv, Washington, DC 20250 USA. [Thorpe, Laurie A.] US Forest Serv, Enterprise Unit, Washington, DC USA. [Williams, Peter B.] Udall Fdn, US Inst Environm Conflict Resolut, Tucson, AZ USA. RP DuPraw, ME (reprint author), Calif State Univ Sacramento, Ctr Collaborat Policy, Sacramento, CA 95819 USA. NR 28 TC 0 Z9 0 U1 1 U2 1 PU WILEY PERIODICALS, INC PI SAN FRANCISCO PA ONE MONTGOMERY ST, SUITE 1200, SAN FRANCISCO, CA 94104 USA SN 1536-5581 EI 1541-1508 J9 CONFL RESOLUT Q JI Confl. Resol. Q. PD WIN PY 2015 VL 33 IS 2 BP 149 EP 176 DI 10.1002/crq.21148 PG 28 WC Social Sciences, Interdisciplinary SC Social Sciences - Other Topics GA DB7NJ UT WOS:000368702000003 ER PT J AU Straub, L Williams, GR Pettis, J Fries, I Neumann, P AF Straub, Lars Williams, Geoffrey R. Pettis, Jeff Fries, Ingemar Neumann, Peter TI Superorganism resilience: eusociality and susceptibility of ecosystem service providing insects to stressors SO CURRENT OPINION IN INSECT SCIENCE LA English DT Article ID AETHIOPICUS SMITH HYMENOPTERA; BEE COLONY LOSSES; NEONICOTINOID PESTICIDES; POPULATION DECLINES; HONEYBEE COLONIES; ANT; QUEENS; CONSERVATION; FORMICIDAE; INCREASES AB Insects provide crucial ecosystem services for human food security and maintenance of biodiversity. It is therefore not surprising that major declines in wild insects, combined with losses of managed bees, have raised great concern. Recent data suggest that honey bees appear to be less susceptible to stressors compared to other species. Here we argue that eusociality plays a key role for the susceptibility of insects to environmental stressors due to what we call superorganism resilience, which can be defined as the ability to tolerate the loss of somatic cells (=workers) as long as the germ line (=reproduction) is maintained. Life history and colony size appear critical for such resilience. Future conservation efforts should take superorganism resilience into account to safeguard ecosystem services by insects. C1 [Straub, Lars; Williams, Geoffrey R.; Neumann, Peter] Univ Bern, Vetsuisse Fac, Inst Bee Hlth, Bern, Switzerland. [Straub, Lars; Williams, Geoffrey R.; Neumann, Peter] Agroscope, Swiss Bee Res Ctr, Bern, Switzerland. [Pettis, Jeff] ARS, USDA, Bee Res Lab, Beltsville, MD USA. [Fries, Ingemar] Swedish Univ Agr Sci, Dept Ecol, S-75007 Uppsala, Sweden. RP Straub, L (reprint author), Univ Bern, Vetsuisse Fac, Inst Bee Hlth, Bern, Switzerland. EM lars.straub@vetsuisse.unibe.ch FU Bundesamt fur Umwelt (BAFU); Agroscope; Vinetum Foundation FX Financial support was granted to LS, GW, and PN by the Bundesamt fur Umwelt (BAFU), by Agroscope to GW and by the Vinetum Foundation to PN. Sincere thanks to the reviewers who provided valuable suggestions. NR 41 TC 6 Z9 6 U1 11 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2214-5745 EI 2214-5753 J9 CURR OPIN INSECT SCI JI Curr. Opin. Insect Sci. PD DEC PY 2015 VL 12 BP 109 EP 112 DI 10.1016/j.cois.2015.10.010 PG 4 WC Biology; Ecology; Entomology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Entomology GA DC1ZV UT WOS:000369017900016 ER PT J AU Caldwell, PV Kennen, JG Sun, G Kiang, JE Butcher, JB Eddy, MC Hay, LE LaFontaine, JH Hain, EF Nelson, SAC McNulty, SG AF Caldwell, Peter V. Kennen, Jonathan G. Sun, Ge Kiang, Julie E. Butcher, Jon B. Eddy, Michele C. Hay, Lauren E. LaFontaine, Jacob H. Hain, Ernie F. Nelson, Stacy A. C. McNulty, Steve G. TI A comparison of hydrologic models for ecological flows and water availability SO ECOHYDROLOGY LA English DT Article DE hydrologic models; environmental flow; calibration; uncertainty; ecosystem health; ELOHA; water supply ID CONTERMINOUS UNITED-STATES; LAND-COVER; REGIME; CONSEQUENCES; BIODIVERSITY; IMPACTS; CLIMATE; STREAM; DAMS AB Robust hydrologic models are needed to help manage water resources for healthy aquatic ecosystems and reliable water supplies for people, but there is a lack of comprehensive model comparison studies that quantify differences in streamflow predictions among model applications developed to answer management questions. We assessed differences in daily streamflow predictions by four fine-scale models and two regional-scale monthly time step models by comparing model fit statistics and bias in ecologically relevant flow statistics (ERFSs) at five sites in the Southeastern USA. Models were calibrated to different extents, including uncalibrated (level A), calibrated to a downstream site (level B), calibrated specifically for the site (level C) and calibrated for the site with adjusted precipitation and temperature inputs (level D). All models generally captured the magnitude and variability of observed streamflows at the five study sites, and increasing level of model calibration generally improved performance. All models had at least 1 of 14 ERFSs falling outside a +/-30% range of hydrologic uncertainty at every site, and ERFSs related to low flows were frequently over-predicted. Our results do not indicate that any specific hydrologic model is superior to the others evaluated at all sites and for all measures of model performance. Instead, we provide evidence that (1) model performance is as likely to be related to calibration strategy as it is to model structure and (2) simple, regional-scale models have comparable performance to the more complex, fine-scale models at a monthly time step. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Caldwell, Peter V.] US Forest Serv, USDA, Ctr Forest Watershed Sci, Otto, NC USA. [Kennen, Jonathan G.] US Geol Survey, Lawrenceville, NJ USA. [Sun, Ge; McNulty, Steve G.] US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC USA. [Kiang, Julie E.] US Geol Survey, Off Surface Water, Reston, VA USA. [Butcher, Jon B.] Tetra Tech, Res Triangle Pk, NC USA. [Eddy, Michele C.] RTI Int, Res Triangle Pk, NC USA. [Hay, Lauren E.] US Geol Survey, Lakewood, CO USA. [LaFontaine, Jacob H.] US Geol Survey, Norcross, GA USA. [Hain, Ernie F.; Nelson, Stacy A. C.] N Carolina State Univ, Dept Forestry & Environm Resources, Ctr Geospatial Analyt, Raleigh, NC 27695 USA. RP Caldwell, PV (reprint author), US Forest Serv, USDA, Ctr Forest Watershed Sci, Coweeta Hydrol Lab, 3160 Coweeta Lab Rd, Otto, NC 28763 USA. EM pcaldwell02@fs.fed.us FU US Department of Agriculture Forest Service Eastern Forest Environmental Threat Assessment Center; Coweeta Hydrologic Laboratory; US Department of Interior, Southeast Climate Science Center; USGS National Water Census was greatly appreciated FX We are indebted to the many individuals and agencies that willingly participated in this modelling study and provided the data and flow model output that was integral to the comparisons. We thank Jerry McMahon for his guidance throughout this project and anonymous reviewers for providing timely reviews that greatly improved the final version of this manuscript. We thank North Carolina State University for providing the facilities for a model comparison workshop among agencies and modellers participating in this study. Jeremy Wyss (Tetra Tech) and Pushpa Tuppad (Texas A&M University) were responsible for calibrating the basin-scale HSPF and SWAT models, respectively. The HSPF and SWAT model applications were supported by the US EPA Office of Research and Development National Center for Environmental Assessment under the guidance of Dr Thomas Johnson; however, the views expressed in this paper represent those of the authors and do not necessarily reflect the views or policies of the US Environmental Protection Agency. Funding for the WaSSI model analysis was provided by the US Department of Agriculture Forest Service Eastern Forest Environmental Threat Assessment Center and the Coweeta Hydrologic Laboratory. Funding for this study and for a model comparison workshop was provided by the US Department of Interior, Southeast Climate Science Center. Additional support from the USGS National Water Census was greatly appreciated. This manuscript has benefitted from peer review from C. Andrew Dolloff (US Forest Service, Center for Forest Watershed Science) and Rodney R. Knight (USGS, Tennessee Water Science Center). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 84 TC 4 Z9 4 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD DEC PY 2015 VL 8 IS 8 BP 1525 EP 1546 DI 10.1002/eco.1602 PG 22 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DB7OS UT WOS:000368705800011 ER PT J AU Scott, RL Biederman, JA Hamerlynck, EP Barron-Gafford, GA AF Scott, Russell L. Biederman, Joel A. Hamerlynck, Erik P. Barron-Gafford, Greg A. TI The carbon balance pivot point of southwestern US semiarid ecosystems: Insights from the 21st century drought SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE drought; semiarid; carbon dioxide; water; ecosystem; net ecosystem production ID DIOXIDE EXCHANGE; INTERANNUAL VARIABILITY; EDDY COVARIANCE; RESOURCE PULSES; PLANT MORTALITY; USE EFFICIENCY; SOIL-MOISTURE; UNITED-STATES; NORTH-AMERICA; GLOBAL-CHANGE AB Global-scale studies indicate that semiarid regions strongly regulate the terrestrial carbon sink. However, we lack understanding of how climatic shifts, such as decadal drought, impact carbon sequestration across the wide range of structural diversity in semiarid ecosystems. Therefore, we used eddy covariance measurements to quantify how net ecosystem production of carbon dioxide (NEP) differed with relative grass and woody plant abundance over the last decade of drought in four Southwest U.S. ecosystems. We identified a precipitation pivot point in the carbon balance for each ecosystem where annual NEP switched from negative to positive. Ecosystems with grass had pivot points closer to the drought period precipitation than the predrought average, making them more likely to be carbon sinks (and a grass-free shrubland, a carbon source) during the current drought. One reason for this is that the grassland located closest to the shrubland supported higher leaf area and photosynthesis at the same water availability. Higher leaf area was associated with a greater proportion of evapotranspiration being transpiration (T/ET), and therefore with higher ecosystem water use efficiency (gross ecosystem photosynthesis/ET). Our findings strongly show that water availability is a primary driver of both gross and net semiarid productivity and illustrate that structural differences may contribute to the speed at which ecosystem carbon cycling adjusts to climatic shifts. C1 [Scott, Russell L.; Biederman, Joel A.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [Hamerlynck, Erik P.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR USA. [Barron-Gafford, Greg A.] Univ Arizona, Sch Geog & Dev, Tucson, AZ USA. [Barron-Gafford, Greg A.] Univ Arizona, Earthsci B2, Biosphere 2, Tucson, AZ USA. RP Scott, RL (reprint author), USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. EM russ.scott@ars.usda.gov FU USDA-ARS; U.S. Department of Energy Berkeley National Labs; University of Arizona FX Data used in this paper are available at the Ameriflux Data Repository (http://ameriflux.lbl.gov/) or upon request to the corresponding author. This work was supported by USDA-ARS and funding for these Ameriflux Core Sites was provided by the U.S. Department of Energy Berkeley National Labs. Funding for these data sets and maintaining the research infrastructure on Walnut Gulch and the Santa Rita Experimental Range was provided by the USDA-ARS and the University of Arizona. We thank R. Bryant for his expert technical assistance in maintaining the eddy covariance sites. USDA-ARS is an equal opportunity employer. NR 73 TC 12 Z9 12 U1 11 U2 24 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD DEC PY 2015 VL 120 IS 12 BP 2612 EP 2624 DI 10.1002/2015JG003181 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DC0WL UT WOS:000368938500009 ER PT J AU Yang, J Tian, HQ Tao, B Ren, W Pan, SF Liu, YQ Wang, YH AF Yang, Jia Tian, Hanqin Tao, Bo Ren, Wei Pan, Shufen Liu, Yongqiang Wang, Yuhang TI A growing importance of large fires in conterminous United States during 1984-2012 SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE burned area; remote-sensed burn severity; fire emissions; climate change; combustion completeness ID NORMALIZED BURN RATIO; PYROGENIC CARBON EMISSIONS; CANADIAN BOREAL FOREST; BLACK SPRUCE FORESTS; GULF-OF-MEXICO; ECOSYSTEM CARBON; TERRESTRIAL ECOSYSTEMS; TEMPORAL PATTERNS; MISSISSIPPI RIVER; LANDSAT IMAGERY AB Fire frequency, extent, and size exhibit a strong linkage with climate conditions and play a vital role in the climate system. Previous studies have shown that the frequency of large fires in the western United States increased significantly since the mid-1980s due to climate warming and frequent droughts. However, less work has been conducted to examine burned area and fire emissions of large fires at a national scale, and the underlying mechanisms accounting for the increases in the frequency of large fires are far from clear. In this study, we integrated remote-sensed fire perimeter and burn severity data sets into the Dynamic Land Ecosystem Model to estimate carbon emissions from large fires (i.e., fires with size larger than 1000acres or 4.05km(2)) in conterminous United States from 1984 to 2012. The results show that average area burned by large fires was 1.44x10(4)km(2)yr(-1) and carbon emissions from large fires were 17.65TgCyr(-1) during the study period. According to the Mann-Kendall trend test, annual burned area and pyrogenic carbon emissions presented significant upward trends at the rates of 810km(2)yr(-1) and 0.87TgCyr(-1), respectively. Characteristic fire size (fire size with the largest contribution to the total burned area) in the period of 2004-2012 increased by 176.1% compared to the period of 1984-1993. We further found that the larger fires were associated with higher burn severity and occurred more frequently in the warmer and drier conditions. This finding implies that the continued warming and drying trends in the 21st century would enhance the total burned area and fire emissions due to the contributions of larger and more severe wildfires. C1 [Yang, Jia; Tian, Hanqin; Tao, Bo; Ren, Wei; Pan, Shufen] Auburn Univ, Int Ctr Climate & Global Change Res, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Tao, Bo; Ren, Wei] Univ Kentucky, Coll Agr Food & Environm, Dept Plant & Soil Sci, Lexington, KY USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Athens, GA USA. [Wang, Yuhang] Georgia Inst Technol, Sch Earth & Atmospher Sci, Atlanta, GA 30332 USA. RP Tian, HQ (reprint author), Auburn Univ, Int Ctr Climate & Global Change Res, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. EM tianhan@auburn.edu RI Ren, Wei/G-8317-2016; Ren, Wei/I-4048-2014; Yang, Jia/A-6483-2012; OI Ren, Wei/0000-0002-4840-4835; Yang, Jia/0000-0003-2019-9603; Tian, Hanqin/0000-0002-1806-4091 FU NSF/USDA/DOE [AGS-1243232, AGS-1243220, NIFC2013-35100-20516]; USDA/USDI [JFSP 11172]; NASA [NNX14AO73G] FX This research has been supported by NSF/USDA/DOE Decadal and Regional Climate Prediction using Earth System Models (AGS-1243232, AGS-1243220, and NIFC2013-35100-20516), USDA/USDI Joint Fire Science Program (JFSP 11172), and NASA Carbon Monitoring System Project (NNX14AO73G). We appreciate the assistance of Brad Quayle at USDA Forest Service and Steve Howard at U.S. Geological Survey with MTBS data. We also thank the valuable and constructive comments from the three anonymous reviewers. The availability of MTBS fire data, climate data, and other driving forces is described in sections 3 and 6. The data sets to validate the model simulation are described in the supporting information. NR 85 TC 2 Z9 2 U1 9 U2 23 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD DEC PY 2015 VL 120 IS 12 BP 2625 EP 2640 DI 10.1002/2015JG002965 PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DC0WL UT WOS:000368938500010 ER PT J AU Reidy, JL O'Donnell, L Thompson, FR AF Reidy, Jennifer L. O'Donnell, Lisa Thompson, Frank R., III TI Evaluation of a Reproductive Index for Estimating Songbird Productivity: Case Study of the Golden-Cheeked Warbler SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE breeding success; color-banded birds; forest songbird; intensive monitoring; pairing success; territory mapping; Vickery index ID NEST SURVIVAL; SUCCESS; DENSITY; FOREST; CONSERVATION; LANDSCAPE; SPARROWS AB It is critically important to determine and understand relationships between endangered species populations and landscape and habitat features to effectively manage and conserve populations and the habitats they rely on. Several recent studies focused on the golden-cheeked warbler (Setophaga chrysoparia), an endangered songbird that breeds exclusively in central Texas, USA, have used a reproductive index to link pairing and breeding success to various factors of interest. However, no field test has evaluated the performance of a reproductive index at estimating productivity of golden-cheeked warblers or similar forest songbirds. We computed reproductive index ranks for 5 observers from multiple plots during 2013 and 2014 and compared these ranks with actual reproductive success determined from territory mapping and nest monitoring of color-banded adults. At the territory level, we found no significant correlation between 84 matched territories delineated by the reproductive index and territories delineated by intensive monitoring. At the plot level, index monitoring underestimated the total number of territorial males, but density from index monitoring was weakly correlated with actual density. We found no significant relationship for pairing success estimated from the reproductive index and actual pairing success, or with breeding success from the reproductive index with nest survival or actual breeding success, when controlling for observer. The reproductive index did not produce reliable estimates of reproductive performance at either the territory or plot level and we recommend intensive monitoring of color-banded individuals when absolute estimates of reproductive success rates or other demographic measures are required, such as in species viability or threat assessments. (C) 2015 The Wildlife Society. C1 [Reidy, Jennifer L.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Nat Resources Bldg, Columbia, MO 65211 USA. [O'Donnell, Lisa] City Austin, Wildland Conservat Div, Austin, TX 78738 USA. [Thompson, Frank R., III] US Forest Serv, No Res Stn, USDA, Columbia, MO 65211 USA. RP Reidy, JL (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 Nat Resources Bldg, Columbia, MO 65211 USA. EM jennifer.reidy@gmail.edu FU U.S. Fish and Wildlife Service; City of Austin, Travis County; U.S. Department of Agriculture Forest Service Northern Research Station; University of Missouri FX We thank the numerous field biologists and volunteers who assisted with data collection. The U.S. Fish and Wildlife Service provided funding for the study. Additional funding, in-kind, and logistical support were provided by the City of Austin, Travis County, the U.S. Department of Agriculture Forest Service Northern Research Station, and the University of Missouri. O. Bocanegra, T. Benson, and 2 anonymous reviewers provided constructive feedback on an earlier version of this manuscript. NR 43 TC 1 Z9 1 U1 3 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD DEC PY 2015 VL 39 IS 4 BP 721 EP 731 DI 10.1002/wsb.576 PG 11 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0FK UT WOS:000368892400006 ER PT J AU Jachowski, DS Washburn, BE Millspaugh, JJ AF Jachowski, David S. Washburn, Brian E. Millspaugh, Joshua J. TI Revisiting the Importance of Accounting for Seasonal and Diel Rhythms in Fecal Stress Hormone Studies SO WILDLIFE SOCIETY BULLETIN LA English DT Article DE fecal glucocorticoid metabolites; mourning dove; Odocoileus virginianus; physiology; stress hormones; white-tailed deer; Zenaida macroura ID WHITE-TAILED DEER; MOURNING DOVES; GLUCOCORTICOIDS; EXCRETION; TIME; CORTICOSTERONE; METABOLITES; SQUIRRELS; RESPONSES; SPARROWS AB Measurement of fecal glucocorticoid metabolites (FGMs) has been used to quantify stress responses by a wide variety of species; yet, few studies attempt to conduct baseline research prior to investigating effects of disturbances on FGM levels. We evaluated the potential confounding effect of seasonal and diel rhythms on the interpretation of FGM values, by studying long-term patterns of FGMs in white-tailed deer (Odocoileus virginianus) and mourning doves (Zenaida macroura) between 2001 and 2002 in a controlled environment at facilities located at the Charles W. Green Conservation Area, near Ashland, Missouri, USA. For 1 year, we collected fresh fecal samples (<1 hr old) on a weekly basis from 17 captive deer and 8 captive doves. We also conducted hourly sampling over discrete 48-hour periods to evaluate diel rhythms. In deer, we observed that FGMs were nearly 2 times higher during spring months of April and May compared with late winter (i.e., Jan and Feb) and late summer (i.e., Jul and Aug). Dove FGMs were 50% higher during the late spring (i.e., May and Jun) compared with early spring and winter months (Jan-Apr), and twice as high as during late summer, autumn, and early winter months of August through December. In both species, we observed considerable variation in diel rhythms that was inconsistent across individuals. Collectively, our findings emphasize the need for researchers to design sampling schemes that account for high levels of individual and temporal variability in baseline FGMs- and consistently ask: What are "normal" FGM values in the context of the animal's environment and prevailing stressors. (C) 2015 The Wildlife Society. C1 [Jachowski, David S.] Clemson Univ, Dept Forestry & Environm Conservat, Clemson, SC 29634 USA. [Washburn, Brian E.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. RP Jachowski, DS (reprint author), Univ KwaZulu Natal, Sch Life Sci, Westville Campus,Private Bag X54001, Durban, South Africa. EM djachowski@gmail.com FU University of Missouri (MU) Department of Fisheries and Wildlife Sciences; MU Life Science Mission Enhancement Postdoctoral Fellowship; MU Research Board Grant; Missouri Department of Conservation (Federal Aid in Wildlife Restoration Project) [W-13-R]; Webless Migratory Game Bird Research Program (U.S. Fish and Wildlife Service and the U.S. Geological Survey-Biological Resources Division) FX Financial and logistical support for this project was provided by the University of Missouri (MU) Department of Fisheries and Wildlife Sciences, a MU Life Science Mission Enhancement Postdoctoral Fellowship, a MU Research Board Grant, the Missouri Department of Conservation (Federal Aid in Wildlife Restoration Project W-13-R), and the 2001 Webless Migratory Game Bird Research Program (U.S. Fish and Wildlife Service and the U.S. Geological Survey-Biological Resources Division). Fecal glucocorticoid assays were conducted in the Wildlife Stress Physiology Laboratory in the Department of Fisheries and Wildlife Sciences at the University of Missouri-Columbia. We thank B. Crampton, S. Kistner, B. Hoenes, T. Bonnot, T. Mong, R. Woods, T. Meyer, L. Miller, J. Schulz, R. Woeck, and C. Rittenhouse for their assistance in pen construction and/or feces collection. We thank the Associate Editor E. Rominger and 2 anonymous reviewers for helpful comments that improved this manuscript. NR 46 TC 2 Z9 2 U1 5 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1938-5463 J9 WILDLIFE SOC B JI Wildl. Soc. Bull. PD DEC PY 2015 VL 39 IS 4 BP 738 EP 745 DI 10.1002/wsb.592 PG 8 WC Biodiversity Conservation SC Biodiversity & Conservation GA DC0FK UT WOS:000368892400008 ER PT J AU Timmermans, WJ Kustas, WP Andreu, A AF Timmermans, Wim J. Kustas, William P. Andreu, Ana TI Utility of an Automated Thermal-Based Approach for Monitoring Evapotranspiration SO ACTA GEOPHYSICA LA English DT Article DE remote sensing; water use monitoring; temperature index scheme; automated; operational ID SURFACE-ENERGY BALANCE; LARGE-APERTURE SCINTILLOMETER; EDDY COVARIANCE MEASUREMENTS; ATMOSPHERIC BOUNDARY-LAYER; REMOTELY-SENSED DATA; HEAT-FLUX; EVAPORATIVE FRACTION; VEGETATION INDEXES; AIR-TEMPERATURE; 2-SOURCE MODEL AB A very simple remote sensing-based model for water use monitoring is presented. The model acronym DATTUTDUT (Deriving Atmosphere Turbulent Transport Useful To Dummies Using Temperature) is a Dutch word which loosely translates as "it's unbelievable that it works". DATTUTDUT is fully automated and only requires a surface temperature map, making it simple to use and providing a rapid estimate of spatially-distributed fluxes. The algorithm is first tested over a range of environmental and land-cover conditions using data from four short-term field experiments and then evaluated over a growing season in an agricultural region. Flux model output is in satisfactory agreement with observations and established remote sensing-based models, except under dry and partial canopy cover conditions. This suggests that DATTUTDUT has utility in identifying relative water use and as an operational tool providing initial estimates of ET anomalies in data-poor regions that would be confirmed using more robust modeling techniques. C1 [Timmermans, Wim J.] Univ Twente, Fac Geoinformat Sci & Earth Observat, Dept Water Resources, POB 217, NL-7500 AE Enschede, Netherlands. [Kustas, William P.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Andreu, Ana] Inst Invest & Formac Agr & Pesquera IFAPA, Cordoba, Spain. RP Timmermans, WJ (reprint author), Univ Twente, Fac Geoinformat Sci & Earth Observat, Dept Water Resources, POB 217, NL-7500 AE Enschede, Netherlands. EM w.j.timmermans@utwente.nl FU NASA Interdisciplinary Research Program in Earth Sciences; EOS/Land Surface Hydrology Program; Terrestrial Hydrology Program; USDA-ARS; European Community's 7th Framework Programme (FP7) under EUFAR [227159]; Cost Action [ES0903-EUROSPEC]; ESA [D/EOP/rp/2012/48] FX Funding from NASA Interdisciplinary Research Program in Earth Sciences, EOS/Land Surface Hydrology Program and the Terrestrial Hydrology Program as well as USDA-ARS funding made possible the experimental data sets from Monsoon'90, SGP'97, and SMACEX'02, respectively. Funding from the European Community's 7th Framework Programme (FP7/2008-2013) under EUFAR contract no. 227159, Cost Action ES0903-EUROSPEC and ESA Grant D/EOP/rp/2012/48 made possible the experimental dataset from the REFLEX'12 Campaign. We would like to thank Dr. Wouter Meijninger and Dr. Ambro Gieske for providing the Gediz data, which made it possible to conduct this study. We also thank Dr. Henk de Bruin and Dr. Martha Anderson for their critical and helpful comments and suggestions. USDA is an equal opportunity provider and employer. NR 80 TC 1 Z9 1 U1 1 U2 7 PU DE GRUYTER OPEN LTD PI WARSAW PA BOGUMILA ZUGA 32A ST, 01-811 WARSAW, POLAND EI 1895-7455 J9 ACTA GEOPHYS JI Acta Geophys. PD DEC PY 2015 VL 63 IS 6 BP 1571 EP 1608 DI 10.1515/acgeo-2015-0016 PG 38 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA DB3RD UT WOS:000368428100007 ER PT J AU Urbina, JA McKerrow, JH AF Urbina, Julio A. McKerrow, James H. TI Drug Susceptibility of Genetically Engineered Trypanosoma cruzi Strains and Sterile Cure in Animal Models as a Criterion for Potential Clinical Efficacy of Anti-T. cruzi Drugs SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Letter ID CHRONIC CHAGAS-DISEASE; IN-VIVO; ETIOLOGIC TREATMENT; INFECTION; POSACONAZOLE C1 [Urbina, Julio A.] Inst Venezolano Invest Cient, Caracas, Venezuela. [McKerrow, James H.] USDA, Skaggs Sch Pharm & Pharmaceut Sci, San Diego, CA USA. RP Urbina, JA (reprint author), Inst Venezolano Invest Cient, Caracas, Venezuela. EM jaurbina@mac.com NR 14 TC 2 Z9 2 U1 1 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0066-4804 EI 1098-6596 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD DEC PY 2015 VL 59 IS 12 BP 7923 EP 7924 DI 10.1128/AAC.01714-15 PG 2 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA DB2KO UT WOS:000368337300110 PM 26578701 ER PT J AU Howard, JK Furnish, JL Box, JB Jepsen, S AF Howard, Jeanette K. Furnish, Joseph L. Box, Jayne Brim Jepsen, Sarina TI The decline of native freshwater mussels (Bivalvia: Unionoida) in California as determined from historical and current surveys SO CALIFORNIA FISH AND GAME LA English DT Article DE Anodonta; California; freshwater mussels; Gonidea angulata; historical and current distribution; Margaritifera falcata ID WESTERN NORTH-AMERICA; UNITED-STATES; MARGARITIFERA-FALCATA; CONSERVATION STATUS; POPULATION; ANODONTA; MOLLUSKS; SEARCH; EXTINCTIONS; STREAMS AB Freshwater mussels are increasingly recognized as important components of aquatic ecosystems but paradoxically are one of the most critically imperiled faunal groups in North America. In California the conservation status of all three native genera had not been comprehensively evaluated in over 30 years. We determined the current distribution of freshwater mussels in California by resurveying historical sites of known occurrences and evaluating the relative change between historical and contemporary surveys. A total of 450 historical records were compiled and represented 116 unique, locatable sites. Nearly 70% of the historical sites were resurveyed, and freshwater mussels were found at 47% of the resurveyed sites. Of the three mussel genera (Anodonta, Gonidea and Margaritifera) known from California, Anodonta was historically the most commonly observed genus, but was only found at 33% of the resurveyed sites. Although Margaritifera and Gonidea were historically found at fewer sites than Anodonta, they were extant at 65% and 55% of the resurveyed sites, respectively. Mussel losses were especially apparent in southern California, with mussels extirpated from 13 of 14 resurveyed sites. The absence of mussels from many historical sites, especially in southern California, parallels the on-going decline of freshwater mussel populations nationally. C1 [Howard, Jeanette K.] Nature Conservancy, 201 Mission St,4th Floor, San Francisco, CA 94105 USA. [Furnish, Joseph L.] US Forest Serv, Pacific Southwest Reg, Vallejo, CA 94592 USA. [Box, Jayne Brim] Umatilla Indian Reservat, Confederated Tribes, Pendleton, OR 97801 USA. [Jepsen, Sarina] Xerces Soc Invertebrate Conservat, Portland, OR 97232 USA. RP Howard, JK (reprint author), Nature Conservancy, 201 Mission St,4th Floor, San Francisco, CA 94105 USA. EM jeanette_howard@tnc.org FU Confederated Tribes of the Umatilla Indian Reservation; USFS PSW Regional Office, Vallejo, California [AG-91S8-C-05-0020, AG-91S8-P-07-0080] FX We thank the Confederated Tribes of the Umatilla Indian Reservation for providing the funding and staff to conduct museum site visits to collect historical records. We are very grateful to Lorrie Haley (Spring Rivers Ecological Sciences) and Steve Holdeman (USFS) for providing site data and survey results, and Laurie Bushman and Christine O'Brien for their work in the field to make this study possible. This study was funded by the USFS PSW Regional Office, Vallejo, California under contracts AG-91S8-C-05-0020 and AG-91S8--P-07-0080. NR 72 TC 0 Z9 0 U1 3 U2 8 PU CALIFORNIA FISH AND GAME EDITOR PI SACRAMENTO PA 1416 NINTH ST, SACRAMENTO, CA 95814 USA SN 0008-1078 J9 CALIF FISH GAME JI Calif. Fish Game PD WIN PY 2015 VL 101 IS 1 BP 8 EP 23 PG 16 WC Fisheries; Zoology SC Fisheries; Zoology GA DB5QK UT WOS:000368568500002 ER PT J AU Walter, JA Johnson, DM Tobin, PC Haynes, KJ AF Walter, Jonathan A. Johnson, Derek M. Tobin, Patrick C. Haynes, Kyle J. TI Population cycles produce periodic range boundary pulses SO ECOGRAPHY LA English DT Article ID GYPSY-MOTH LEPIDOPTERA; FOREST-DEFOLIATING INSECT; INVADING ORGANISMS; BIOLOGICAL-CONTROL; LYMANTRIA-DISPAR; NORTH-AMERICA; GEOGRAPHICAL VARIATION; INVASION THEORY; MATING SUCCESS; OUTBREAKS AB Classical theories of biological invasions predict constant rates of spread that can be estimated from measurable life history parameters, but such outcomes depend strongly on assumptions that are often unmet in nature. Subsequent advances have demonstrated how relaxing assumptions of these foundational models results in other spread patterns seen in nature, including invasions that accelerate through time, or that alternate among periods of expansion, retraction, and stasis of range boundaries. In this paper, we examine how periodic population fluctuations affect temporal patterns of range expansion by coupling empirical data on the gypsy moth invasion in North America with insights from a model incorporating population cycles, Allee effects, and stratified diffusion. In an analysis of field data, we found that gypsy moth spread exhibits pulses with a period of 6 yr, which field data and model simulations suggest is the result of a 6-yr population cycle in established populations near the invasion front. Model simulations show that the development of periodic behavior in range expansion depends primarily on the period length of population cycles. The period length of invasion pulses corresponded to the population cycle length, and the regularity of invasion pulses tended to decline with increases in population cycle length. A key insight of this research is that dynamics of established populations, behind the invasion front, can have strong effects on spread. Our findings suggest that coordination between separate management programs targeting low-density spreading and established outbreaking populations, respectively, could increase the efficacy of efforts to mitigate gypsy moth impacts. Given the variety of species experiencing population fluctuations, Allee effects, and stratified diffusion, insights from this study are potentially important to understanding how the range boundaries of many species change. C1 [Walter, Jonathan A.] Univ Virginia, Dept Environm Sci, Clark Hall, Charlottesville, VA 22903 USA. [Walter, Jonathan A.; Haynes, Kyle J.] Univ Virginia, Blandy Expt Farm, Boyce, VA USA. [Walter, Jonathan A.; Johnson, Derek M.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Tobin, Patrick C.] USDA, No Res Stn, Morgantown, WV USA. RP Walter, JA (reprint author), Univ Virginia, Dept Environm Sci, Clark Hall, Charlottesville, VA 22903 USA. EM jwalter4@vcu.edu RI Johnson, Derek/B-6409-2012 FU Univ. of Virginia Dept of Environmental Sciences; Blandy Experimental Farm; USDA Forest Service, Northeastern Area, State and Private Forestry [13-CA-11420004-231] FX The authors would like to thank Laura Blackburn (USDA Forest Service) for technical assistance and the gypsy moth Slow the Spread Foundation, for access to data. This work was supported by the Univ. of Virginia Dept of Environmental Sciences, Blandy Experimental Farm, and Domestic Cooperative Agreement 13-CA-11420004-231 award by the USDA Forest Service, Northeastern Area, State and Private Forestry to DMJ. NR 73 TC 1 Z9 1 U1 8 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-7590 EI 1600-0587 J9 ECOGRAPHY JI Ecography PD DEC PY 2015 VL 38 IS 12 BP 1200 EP 1211 DI 10.1111/ecog.01364 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DB3WS UT WOS:000368444600004 ER PT J AU Valles, SM Wetterer, JK Porter, SD AF Valles, Steven M. Wetterer, James K. Porter, Sanford D. TI The red imported fire ant (Hymenoptera: Formicidae) in the West Indies: distribution of natural enemies and a possible test bed for release of self-sustaining biocontrol agents SO FLORIDA ENTOMOLOGIST LA English DT Article DE Kneallhazia solenopsae; Vairimorpha invictae; SINV; SiDNV; Pseudacteon; pathogen; parasite ID SOLENOPSIS-INVICTA HYMENOPTERA; VIRUS 3; RHINOCEROS BEETLE; SUCCESSFUL TRANSMISSION; BIOLOGICAL-CONTROL; HOST-SPECIFICITY; MULTIPLEX PCR; POLYGYNE; MICROSPORIDIA; THELOHANIIDAE AB Sample collections of Solenopsis invicta Buren (Hymenoptera: Formicidae) were taken from 20 islands of the West Indies and evaluated for the presence of key pathogens and parasites of this invasive pest ant. We hypothesized that bottleneck events during the introduction of this ant species in the West Indies would have resulted in populations devoid, or nearly so, of natural enemies. Monogyne and polygyne social forms were found throughout the islands surveyed with monogyny being more prevalent (65%) compared with polygyny (35%). Among 254 samples, only 25 (similar to 10%) tested positive for the presence of pathogens or parasites. The microsporidian Kneallhazia solenopsae was the most prevalent pathogen detected; it was found in 20 colonies. A second microsporidian species, Vairimorpha invictae, was shown to be present in a polygyne sample collected from St. Croix-the first detection of this pathogen outside South America. Similarly, Solenopsis invicta densovirus (SiDNV) was detected in one polygyne sample from Anguilla. SiDNV is not found in S. invicta U. S. populations, so this detection also represents the first geographic discovery outside of South America. Two species of Pseudacteon decapitating flies were found to have dispersed into the Bahamas. Utilization of the islands of the West Indies for release, establishment, and impact assessment of S. invicta natural enemies is discussed. C1 [Valles, Steven M.; Porter, Sanford D.] USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. [Wetterer, James K.] Florida Atlantic Univ, Wilkes Honors Coll, Jupiter, FL 33458 USA. RP Valles, SM (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 49 TC 1 Z9 1 U1 7 U2 19 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1101 EP 1105 PG 5 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000013 ER PT J AU Johnson, CW MacRae, TC Brownie, C Virgets, W Allison, JD AF Johnson, C. Wood MacRae, Ted C. Brownie, Cavell Virgets, Warren, III Allison, Jeremy D. TI Observations of Cerceris fumipennis (Hymenoptera: Crabronidae) phenology and variation in its buprestid prey in Louisiana SO FLORIDA ENTOMOLOGIST LA English DT Article DE biosurveillance; Buprestidae; Agrilus planipennis; Coleoptera ID EMERALD ASH BORER; AGRILUS-PLANIPENNIS; NORTH-AMERICA; COLEOPTERA BUPRESTIDAE; DIGGER WASP; SPHECIDAE; NESTS; SOLITARY; INVASION; ECOLOGY AB The non-native emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), threatens extirpation of susceptible ash (Fraxinus species; Lamiales: Oleaceae) in North America. Cerceris fumipennis Say (Hymenoptera: Crabronidae), a ground-nesting wasp that preys on Buprestidae in eastern North America, is used as a survey tool for the emerald ash borer in the northeastern U.S. and Canada. The recent detection of the emerald ash borer in Louisiana provides an opportunity to complement trapping surveys with the use of C. fumipennis, but knowledge of C. fumipennis in the region is lacking. From 2011 to 2014, we conducted searches at 155 sites and located C. fumipennis aggregations at 25% (n = 39) of these sites; 36% (n = 14) of these were located at forest harvests, an aggregation habitat not previously reported in the literature. We collected 1,559 buprestids representing 35 species from 2 aggregations in Louisiana between May and Aug 2012. Buprestid collections at these aggregations and observations of C. fumipennis activity at a 3rd aggregation indicated the number of buprestid species and individuals collected declined significantly from May to Jul. We collected significantly more Agrilus difficilis Gory (Coleoptera: Buprestidae) in the afternoon than morning hours and observed similar diurnal patterns among other buprestid species during the early weeks following aggregation activation. We also discuss evidence suggesting a portion of the regional C. fumipennis population is bivoltine. Although A. planipennis was not collected during this study, our results suggest that C. fumipennis is a feasible sampling tool and a useful addition to ongoing emerald ash borer surveys in the region. C1 [Johnson, C. Wood] US Forest Serv, USDA, Forest Hlth Protect, 2500 Shreveport Hwy, Pineville, LA 71360 USA. [MacRae, Ted C.] Monsanto Co, Chesterfield, MO 63017 USA. [Brownie, Cavell] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. [Virgets, Warren, III] Louisiana Dept Wildlife & Fisheries, Hammond Field Off, Hammond, LA 70403 USA. [Allison, Jeremy D.] Great Lakes Forestry Ctr, Nat Resources Canada Canadian Forest Serv, Marie, ON P6A 2E5, Canada. RP Johnson, CW (reprint author), US Forest Serv, USDA, Forest Hlth Protect, 2500 Shreveport Hwy, Pineville, LA 71360 USA. EM woodjohnson@fs.fed.us FU USDA Forest Service, Forest Health Technology Enterprise Team [10-DG-11083150-014] FX The authors thank Chris Carlton and Victoria Bayless, Louisiana State Arthropod Museum, Baton Rouge, Louisiana, USA, for support, insect identification assistance, and specimen curation. We also thank the Recreation and Parks Commission of East Baton Rouge Parish, Paxton Tedley of Verda Elementary School and Brent Deen with Roy O. Martin Corporation, Chopin, Louisiana, for the use of their properties to conduct this research. We also are indebted to Steve Clarke (United States Department of Agriculture [USDA], Forest Service, Forest Health Protection) for facilitating the cooperation with Texas Master Naturalists Jennifer Hess, Phillip Cuneo, and Teri MacArthur (Spring Creek Chapter), Kathleen Applebaum and Susan Tullos (Piney Wood Lakes Chapter), and Lori Horne ( Longleaf Ridge Chapter) in monitoring aggregations in eastern Texas. Last, but not least, we thank US Forest Service employees Valli Peacher, Susan Stanley, Ron Kertz, Jim Meeker, Alex Mangini, Chris Steiner, and Billy Bruce for assistance with field work. We thank the USDA Forest Service, Forest Health Technology Enterprise Team for providing funding for this work through Special Technology Development Project Grant 10-DG-11083150-014. NR 41 TC 0 Z9 0 U1 1 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1106 EP 1113 PG 8 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000014 ER PT J AU Tillman, PG Greenstone, MH Hu, JS AF Tillman, P. Glynn Greenstone, Matthew H. Hu, Jing S. TI Predation of stink bugs (Hemiptera: Pentatomidae) by a complex of predators in cotton and adjoining soybean habitats in Georgia, USA SO FLORIDA ENTOMOLOGIST LA English DT Article DE PCR-based gut-content analysis; Euschistus; Nezara; Geocoris; Orius; crop-to-crop dispersal ID HETEROPTERA-PENTATOMIDAE; NATURAL ENEMIES; NEZARA-VIRIDULA; PODISUS-MACULIVENTRIS; GEOCORIS-PUNCTIPES; RUBIDIUM MARKING; PEST; EGGS; PREY; INSECTICIDES AB Stink bugs (Hemiptera: Pentatomidae) are economic pests of cotton and soybean. This study was conducted to examine predation on stink bugs by arthropod predators in cotton and adjoining soybean habitats. Gut-content analysis based on polymerase chain reaction (PCR) was used to detect stink bug deoxyribonucleic acid (DNA) in predators collected from both crops over a 5 wk period. Nezara viridula (L.), Euschistus servus (Say), Chinavia hilaris (Say), and Euschistus quadrator Rolston were detected on soybean and cotton. Piezodorus guildinii (Westwood) and Thyanta custator custator (F.) were detected only on soybean whereas Euschistus tristigmus (Say) was detected only on cotton. Over both crops, 13 predators screened positive for a variety of stink bug species DNA by PCR analysis: Geocoris punctipes (Say) and Geocoris uliginosus (Say) (Hemiptera: Geocoridae), Orius insidiosus (Say) (Hemiptera: Anthocoridae), Hippodamia convergens Guerin-Meneville, Harmonia axyridis (Pallas) (cotton), and Scymnus sp. (cotton) (Coleoptera: Coccinellidae), Oxyopes salticus Hentz and Peucetia viridans (Hentz) (cotton) (Araneae: Oxyopidae), Solenopsis invicta Buren (Hymenoptera: Formicidae), Podisus maculiventris (Say) (Hemiptera: Pentatomidae), Mecaphesa asperata (Hentz) (Araneae: Thomisidae), Zelus renardii Kolenati (Hemiptera: Reduviidae), and Notoxus monodon (F.) (cotton) (Coleoptera: Anthicidae). In soybean, the percentage of G. punctipes and G. uliginosus screening positive for N. viridula was high, 87.3%, whereas the percentage screening positive for E. servus was moderately high, 60.3%. In cotton, the percentage of N. viridula DNA in gut-contents of O. insidiosus was high, 91.6%. Detection of P. guildinii and/or T.c. custator DNA in predators in cotton and of E. tristigmus DNA in predators in soybean demonstrated predator dispersal between soybean and cotton. In soybean, the percentage of P. guildinii DNA in gut contents of G. punctipes, G. uliginosus, and O. insidiosus, including those individuals in cotton that dispersed from soybean, was high. We conclude that a complex of arthropod predators prey on a complex of stink bugs in both cotton and adjoining soybean while foraging in and between these crops. C1 [Tillman, P. Glynn] ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. [Greenstone, Matthew H.; Hu, Jing S.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Tillman, PG (reprint author), ARS, USDA, Crop Protect & Management Res Lab, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 52 TC 1 Z9 1 U1 7 U2 27 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1114 EP 1126 PG 13 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000015 ER PT J AU Shelly, TE Epsky, ND AF Shelly, Todd E. Epsky, Nancy D. TI Exposure to tea tree oil enhances the mating success of male Mediterranean fruit flies (Diptera: Tephritidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE Ceratitis capitata; plant-insect interaction; female choice; pheromone calling ID GINGER ROOT OIL; CERATITIS-CAPITATA DIPTERA; MELALEUCA-ALTERNIFOLIA; ALPHA-COPAENE; FLY DIPTERA; COMPETITIVENESS; PERFORMANCE; ATTRACTION; DORSALIS AB The aroma of various plant essential oils has been shown to enhance the mating competitiveness of males of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Laboratory observations revealed that male medflies show strong short-range (<10 cm) attraction to tea tree oil (TTO hereafter) derived from leaves of the Australian plant Melaleuca alternifolia (Maiden & Betch) Cheel (Myrtales: Melastomataceae). The present study was undertaken to i) compare the attractiveness of TTO with that of trimedlure (the male lure routinely used in detection surveys) in field and field cage tests and ii) assess the influence of TTO exposure on male mating success under conditions of varying dose, duration of post-exposure (i.e., pre-test) interval, and access (contact possible or not) to the TTO source. Results showed that TTO-baited traps captured 50% as many males as trimedlure-baited traps in field cages but only 8% as many males as trimedlure-baited traps in the open field. Males exposed to pure TTO or dilutions of 50% and 5% TTO in hexane had higher mating success than non-exposed control males in tests conducted 1 d after exposure. TTO-exposed males also had a mating advantage when tested 3 d after exposure and when physical contact with the TTO source was prevented. In an additional experiment, TTO exposure was found to enhance the mating competitiveness of mass-reared, sterile males in competition against wild males for copulations with wild females in tests conducted 1 or 3 d after exposure. C1 [Shelly, Todd E.] US Anim & Plant Hlth Inspect Serv, USDA, 41-650 Ahiki St, Waimanalo, HI 96795 USA. [Epsky, Nancy D.] ARS, Subtrop Hort Res Stn, USDA, Miami, FL 33158 USA. RP Shelly, TE (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 33 TC 0 Z9 0 U1 3 U2 13 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1127 EP 1133 PG 7 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000016 ER PT J AU Pena, JE Santos, K Baez, I Carrillo, D AF Pena, Jorge E. Santos, Katia Baez, Ignacio Carrillo, Daniel TI Physical post-harvest techniques as potential quarantine treatments against Brevipalpus yothersi (Acarina: Tenuipalpidae) SO FLORIDA ENTOMOLOGIST LA English DT Article DE citrus leprosis virus; CiLV; mite reduction; infested citrus ID CITRUS LEPROSIS; RESISTANCE; FUMIGANT; FRUIT AB Brevipalpus mites (Acari: Tenuipalpidae) carrying citrus leprosis virus are considered serious quarantine pests. The objective of this research was to clarify the effectiveness of commonly used fruit cleaners, soaps, waxes, and mechanical brushing techniques (alone and in combination) on removal and/or mortality of mites (percentage of density reduction) from infested citrus fruits. Six bioassays were conducted with infested lemons, Citrus limon (L.) Burm. f. (Sapindales: Rutaceae), using non-virulent Brevipalpus yothersi Baker as a model species. In each bioassay, all stages (eggs, nymphs, and adults) of B. yothersi were recorded before and after treatment. Results indicated that none of the treatments provided 100% reduction of all stages of mites, as would be required for quarantine treatments. In general, mite reduction following single treatments (soap rinse, brushing, or waxing alone) was not significantly different from reduction obtained with a water drench control. However, several combination treatments were successful in achieving similar to 90% reduction of mites, particularly those that included application of a food-grade wax coating. Therefore, a combination of treatments, including a soap wash and mechanical brushing followed by a wax coating, may be the most effective method to achieve significant reduction of all stages of Brevipalpus mites from infested citrus. C1 [Pena, Jorge E.; Santos, Katia; Carrillo, Daniel] Univ Florida, Trop Res & Educ Ctr, 18905 SW 280th St, Homestead, FL 33031 USA. [Baez, Ignacio] USDA, APHIS CPHST AQI Lab, Raleigh, NC 27606 USA. RP Pena, JE (reprint author), Univ Florida, Trop Res & Educ Ctr, 18905 SW 280th St, Homestead, FL 33031 USA. EM jepena@ufl.edu FU APHIS PPQ grant FX We thank A. Roda (APHIS-PPQ), Paul Kendra (USDA-ARS), and J. C. Rodrigues-Verle (University of Puerto Rico) for their suggestions to improve this manuscript. This work was supported by an APHIS PPQ grant to J. E. P. Note: Mention of proprietary products does not constitute endorsement for their exclusive use by the University of Florida and USDA. NR 16 TC 0 Z9 0 U1 4 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1169 EP 1174 PG 6 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000021 ER PT J AU Lake, EC Gates, MW Smith, MC Witkus, GL Pratt, PD AF Lake, Ellen C. Gates, Michael W. Smith, Melissa C. Witkus, Gloria L. Pratt, Paul D. TI First report of an egg parasitoid reared from Neomusotima conspurcatalis (Lepidoptera: Crambidae), a biological control agent of Lygodium microphyllum (Schizaeales: Lygodiaceae) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID WORLD CLIMBING FERN; INVASIVE WEED; TRICHOGRAMMA-MINUTUM; NATURAL ENEMIES; FLORIDA; HYMENOPTERA; AUSTRALIA; LESSONS; ASIA C1 [Lake, Ellen C.; Smith, Melissa C.] USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. [Gates, Michael W.] USDA ARS, Systemat Entomol Lab, Natl Museum Nat Hist, Washington, DC 20013 USA. [Pratt, Paul D.] USDA ARS, Exot & Invas Weeds Res Unit, Albany, CA 94710 USA. RP Lake, EC (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM Ellen.Lake@ars.usda.gov NR 31 TC 1 Z9 1 U1 3 U2 10 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1244 EP 1246 PG 3 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000035 ER PT J AU Gillett-Kaufman, JL Allan, SA Buss, LJ AF Gillett-Kaufman, Jennifer L. Allan, Sandra A. Buss, Lyle J. TI Manduca rustica (Lepidoptera: Sphingidae) damage on olive (Olea europaea; Lamiales: Oleaceae) trees in Florida SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE rustic sphinx; feeding damage; Cotesia congregata AB The rustic sphinx Manduca rustica (F.) (Lepidoptera: Sphingidae) has been identified as an occasional pest for olive Olea europaea L. (Lamiales: Oleaceae) in Florida, USA. Reports of this pest's distribution on olive trees in Florida range from the northern Panhandle as far south as Polk County. C1 [Gillett-Kaufman, Jennifer L.; Buss, Lyle J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Allan, Sandra A.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Gillett-Kaufman, JL (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM gillett@ufl.edu NR 7 TC 0 Z9 0 U1 1 U2 1 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1260 EP 1261 PG 2 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000041 ER PT J AU Fraedrich, SW Johnson, CW Menard, RD Harrington, TC Olatinwo, R Best, GS AF Fraedrich, Stephen W. Johnson, C. Wood Menard, Roger D. Harrington, Thomas C. Olatinwo, Rabiu Best, G. Susan TI First report of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) and laurel wilt in Louisiana, USA: the disease continues westward on sassafras SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Raffaelea lauricola; redbay ambrosia beetle ID REDBAY AMBROSIA BEETLE; SOUTHEASTERN UNITED-STATES; RAFFAELEA-LAURICOLA; MANUKA OIL; LAURACEAE; SYMBIONT; PATHOGEN; VECTOR; TREES AB Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae) and sassafras trees that died from laurel wilt were discovered in a 3-parish area of northern Louisiana, USA, in Sep 2014. Redbay, a species that has been severely affected by the disease in the coastal plains forests of the southeastern USA, is not found in this area of northern Louisiana, suggesting that sassafras is attractive to X. glabratus and an adequate reproductive host for the beetle. This is the first report of X. glabratus and laurel wilt in Louisiana and the first report of the beetle and disease west of the Mississippi River. C1 [Fraedrich, Stephen W.; Best, G. Susan] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Johnson, C. Wood; Menard, Roger D.] US Forest Serv, USDA, Forest Hlth Protect, Pineville, LA 71360 USA. [Harrington, Thomas C.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Olatinwo, Rabiu] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. RP Fraedrich, SW (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM sfraedrich@fs.fed.us NR 21 TC 3 Z9 3 U1 1 U2 10 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD DEC PY 2015 VL 98 IS 4 BP 1266 EP 1268 PG 3 WC Entomology SC Entomology GA DB7VO UT WOS:000368725000043 ER PT J AU Ding, W Zhang, Y Kou, LP Jurick, WM AF Ding, Wu Zhang, Yao Kou, Liping Jurick, Wayne M., II TI ELECTRONIC NOSE APPLICATION FOR THE DETERMINATION OF PENICILLIN G IN SAANEN GOAT MILK WITH FISHER DISCRIMINATE AND MULTILAYER PERCEPTRON NEURAL NETWORK ANALYSES SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; VOLATILE COMPOUNDS; CHICKEN MUSCLE; PREDICTION; RESIDUES; DOXYCYCLINE; EVOLUTION; QUALITY; RIVER AB Antibiotics are routinely added to milk products and pose potential harm to public health. The objective of this study was to use an innovative and nondestructive application of an electronic nose instrument for rapid detection of penicillin G in goat milk. The PEN3 electronic nose system was utilized to detect volatile substances in goat milk after the addition of penicillin G sodium salt at concentrations of 0, 50, 100 and 200 mu g/L. The data were extracted at 60 s to carry out a linear discriminant analysis. Additional statistical analysis was conducted using neural networks to predict the penicillin G concentration in goat milk samples. Accuracy rates for the two methods were 98.0 and 96.7% for training samples, and 97.0 and 94.9% for testing samples, respectively. The results from this study show that the electronic nose system can be utilized to predict the penicillin G concentrations in goat milk samples. C1 [Ding, Wu; Zhang, Yao; Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Peoples R China. [Jurick, Wayne M., II] USDA ARS, Food Qual Lab, Beltsville, MD USA. RP Ding, W (reprint author), Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Peoples R China. EM dingwu10142000@hotmail.com FU Shaanxi Provincial Natural Science Foundation of China [K332020916] FX This study was supported by Shaanxi Provincial Natural Science Foundation of China under Grant No. K332020916. NR 34 TC 0 Z9 0 U1 6 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 927 EP 932 DI 10.1111/jfpp.12305 PG 6 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300043 ER PT J AU Sang, WG Shao, XF Jin, ZT AF Sang, Weiguo Shao, Xingfeng Jin, Z. Tony TI TEXTURE ATTRIBUTES, RETROGRADATION PROPERTIES AND MICROBIOLOGICAL SHELF LIFE OF INSTANT RICE CAKE SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID DIFFERENTIAL SCANNING CALORIMETRY; STARCH; KINETICS AB Texture attributes, retrogradation properties and microbiological shelf life of instant rice cake were studied. Moisture content, pH, total plate counts, and mold and yeast counts were monitored at 20 and 30C for 90 days, and texture profiles and differential scanning calorimetry (DSC) characteristics were determined at 4, 20 and 30C for 21 days. No significant change in moisture content of rice cake was observed, but the pH values decreased significantly (P < 0.05) during storage. The microbiological shelf life of rice cake was over 50 days at 20C and over 30 days at 30C. Lowering storage temperature increased the hardness and chewiness and decreased the adhesiveness, but did not affect the springiness of rice cake. The DSC data indicated an increase in the enthalpy of the endotherms during storage and lower storage temperature resulted in more change in the enthalpy of the endotherms, indicating different degrees of starch retrogradation during storage. C1 [Sang, Weiguo; Shao, Xingfeng] Ningbo Univ, Dept Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China. [Jin, Z. Tony] USDA, Eastern Reg Res Ctr, Agr Res Serv, Wyndmoor, PA USA. RP Sang, WG (reprint author), Ningbo Univ, Dept Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China. EM sangweiguo@nbu.edu.cn RI Shao, Xingfeng/I-4150-2014; OI Shao, Xingfeng/0000-0002-0724-8186; Jin, Tony/0000-0003-0504-5817 FU Ningbo Science and Technology Bureau of China [200603C1011045] FX This study was part of a research project sponsored by the Ningbo Science and Technology Bureau of China ( Project No. 200603C1011045). The financial support is greatly appreciated. The authors thank Dr. James Smith for his thoughtful review of this manuscript. NR 20 TC 0 Z9 0 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 1832 EP 1838 DI 10.1111/jfpp.12418 PG 7 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300140 ER PT J AU Patel, J Keelara, S Kumar, V AF Patel, Jitendra Keelara, Shivaramu Kumar, Venkitanarayanan TI REDUCTION OF ESCHERICHIA COLI O157:H7 AND SALMONELLA ON FRESH-CUT PRODUCE BY CAPRYLIC ACID SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID LISTERIA-MONOCYTOGENES; FOODBORNE ILLNESS; UNITED-STATES; NONCULTURABLE STATE; ALFALFA SEEDS; MILK LIPIDS; FATTY-ACIDS; O157/H7; INACTIVATION; PATHOGENS AB Caprylic acid (CA) was evaluated for reducing Escherichia coli O157:H7 and Salmonella on fresh produce. Spinach, romaine lettuce and iceberg lettuce were inoculated with a cocktail of five E. coli O157:H7 or Salmonella strains, air dried for 30 min and then dipped in CA (10, 25 and 50 ppm) or chlorine (50 ppm) for 60 s. Treated leaves were analyzed for E. coli O157:H7 and Salmonella following treatment and during storage at 4C for 14 days. The CA treatment significantly reduced these pathogens on fresh produce compared to treatment with water (control) and chlorine. E. coli O157:H7 and Salmonella were undetectable in 25 and 50 ppm CA- treated leaves. E. coli O157:H7 and Salmonella were reduced further during storage. The antibacterial activity of CA (5 ppm) was dependent on exposure time. The CA could be used to kill E. coli O157:H7 and Salmonella on fresh produce. C1 [Patel, Jitendra] USDA ARS, Environm Microbial & Food Safety Lab, BARC, Beltsville, MD 20705 USA. [Keelara, Shivaramu] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Kumar, Venkitanarayanan] Univ Connecticut, Dept Anim Sci, Storrs, CT USA. RP Patel, J (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, BARC, 10300 Baltimore Ave,Bldg 201 BARC East, Beltsville, MD 20705 USA. EM jitu.patel@ars.usda.gov NR 31 TC 0 Z9 0 U1 3 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 2234 EP 2239 DI 10.1111/jfpp.12468 PG 6 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300186 ER PT J AU Bassinello, PZ Carvalho, AV Rios, AD Maciel, RD Berrios, JD AF Bassinello, Priscila Zaczuk Carvalho, Ana Vania Rios, Alessandro De Oliveira Maciel, Renan De Almeida Berrios, Jose De J. TI EXPANDED GLUTEN-FREE EXTRUDATES MADE FROM RICE GRITS AND BANDINHA (BEAN) FLOUR MIXES: MAIN QUALITY PROPERTIES SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID EXTRUSION-COOKING; DIETARY FIBER; EXPANSION; STARCH; PRODUCTS; MODEL AB The effect of extrusion operating conditions on main quality properties of glutenfree expanded snack-type products, developed from rice grits and bandinha flour formulations, was studied. The protein content in the extrudate varied from 9.44 to 14.74 g/100 g for formulations containing 13 and 47% bandinha flour, respectively, processed at 85C and 15% feed moisture. Apparent density and the radial expansion index of the extrudate were significantly (P < 0.05) affected by bean flour content, moisture content, temperature and the interaction between moisture content x temperature. The most suitable extrusion conditions were 30 g/100 g bean flour content, 14 g/100 g moisture content and 80C die temperature, which resulted in a product with a high radial expansion index and crude protein of 9.5 and 13 g/100 g, respectively, and a low apparent density of 0.20 g/cm(3). C1 [Bassinello, Priscila Zaczuk] Empresa Brasileira Pesquisa Agr EMBRAPA, Rice & Beans Res Ctr, Santo Antonio De Gois, Goias, Brazil. [Carvalho, Ana Vania] Embrapa Eastern Amazon Res Ctr, Food Proc Lab, Belem, Para, Brazil. [Rios, Alessandro De Oliveira] Univ Fed Rio Grande do Sul, Food Sci, Porto Alegre, RS, Brazil. [Maciel, Renan De Almeida] Fed Univ Para, Pharm, BR-66059 Belem, Para, Brazil. [Berrios, Jose De J.] USDA ARS, WRRC, Hlth Processed Foods Res Unit, Albany, CA 94710 USA. RP Berrios, JD (reprint author), USDA ARS, WRRC, Hlth Processed Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM jose.berrios@ars.usda.gov NR 27 TC 1 Z9 1 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 2267 EP 2275 DI 10.1111/jfpp.12472 PG 9 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300190 ER PT J AU Zhang, QQ Mukhopadhyay, S Hwang, CA Xu, XL Juneja, VK AF Zhang, Qiuqin Mukhopadhyay, S. Hwang, C. A. Xu, Xinglian Juneja, V. K. TI MODELING THE SURVIVAL OF SALMONELLA ON SLICE COOKED HAM AS A FUNCTION OF APPLE SKIN POLYPHENOLS, ACETIC ACID, OREGANO ESSENTIAL OIL AND CARVACROL SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID HIGH HYDROSTATIC-PRESSURE; ESCHERICHIA-COLI O157-H7; GRAPE SEED EXTRACT; L. ESSENTIAL OIL; LISTERIA-MONOCYTOGENES; STAPHYLOCOCCUS-AUREUS; ANTIMICROBIAL ACTIVITY; PREDICTIVE MODEL; SODIUM-CHLORIDE; ORGANIC-ACIDS AB Response surface methodology was applied to investigate the combined effect of apple skin polyphenols (ASP), acetic acid (AA), oregano essential oil (O) and carvacrol (C) on the inactivation of Salmonella on sliced cooked ham. A full factorial experimental design was employed with control variables of ASP (0-10%), AA (0-4%), O (0-0.6%) and C (0-0.8%). AA, O and C were more effective in reducing Salmonella population densities on sliced cooked ham as compared with ASP; the reductions ranged from 1.2 and 4.4 log colony-forming unit (cfu)/cm(2) for 1 and 4% AA, respectively, to virtually no reduction for 5 and 10% ASP. The interaction between ASP and AA, ASP and C, AA and C also had a significant influence on Salmonella reduction on sliced cooked ham. The highest reduction (6.9 log cfu/cm(2)) was achieved with the use of AA (4%) and C (0.8%) and the least effective was the use of a combination of ASP (10%) and O (0.6%) when the reduction was minimal, i.e., 1.7 log cfu/cm(2). A second-order response surface model developed to predict Salmonella survival was found to be significant (P < 0.0001) with regression coefficients of 0.858 and an insignificant lack of fit (P = 0.4266). Results of this study will assist food processors and regulators in developing guidelines applicable to reducing Salmonella on ready-to-eat foods by combined use of ASP, AA, O and C. C1 [Zhang, Qiuqin; Xu, Xinglian] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China. [Mukhopadhyay, S.; Hwang, C. A.; Juneja, V. K.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Juneja, VK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM vijay.juneja@ars.usda.gov NR 33 TC 3 Z9 3 U1 5 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 2371 EP 2378 DI 10.1111/jfpp.12486 PG 8 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300202 ER PT J AU Denton, JJ Ravishankar, S Friedman, M Jaroni, D AF Denton, Jordan J. Ravishankar, Sadhana Friedman, Mendel Jaroni, Divya TI EFFICACY OF PLANT-DERIVED COMPOUNDS AGAINST ESCHERICHIA COLI O157:H7 DURING FLUME-WASHING AND STORAGE OF ORGANIC LEAFY GREENS SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID RESISTANT SALMONELLA-ENTERICA; LISTERIA-MONOCYTOGENES; ANTIMICROBIAL ACTIVITY; ESSENTIAL OILS; CAMPYLOBACTER-JEJUNI; CHLORINATED WATER; O157-H7; CARVACROL; LETTUCE; CINNAMALDEHYDE AB The antimicrobial efficacy of plant-derived compounds (cinnamaldehyde, carvacrol and citral) against Escherichia coli O157:H7 was evaluated during the flume-tank-washing process and subsequent storage of organic leafy greens. Organic baby and mature spinach, and romaine and iceberg lettuce, inoculated with a cocktail of E. coli O157:H7, were washed for 1 or 2 min in 0.1, 0.3 and 0.5% concentrations of the compounds and stored at 4C. Pathogen survivors were enumerated on days 0, 1 and 3. Significant (P < 0.05) reductions in E. coli O157:H7 populations were observed with all three compounds compared with the controls. Carvacrol at 0.5% was most effective on each leafy green, reducing pathogen populations to undetectable levels on day 0. All of the compounds showed better antimicrobial efficacy at higher concentrations and continued this effect during the 3-day storage. Washing the leafy greens for more than 1 min did not significantly affect the E. coli O157:H7 populations. C1 [Denton, Jordan J.; Jaroni, Divya] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA. [Ravishankar, Sadhana] Univ Arizona, Sch Anim & Comparat Biomed Sci, Tucson, AZ USA. [Friedman, Mendel] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Jaroni, D (reprint author), Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA. EM divya.jaroni@okstate.edu OI Friedman, Mendel/0000-0003-2582-7517 FU United States Department of Agriculture-National Institute of Food and Agriculture-Organic Research and Extension Initiative Competitive Grant [2010-513000-21760] FX This research was supported by the United States Department of Agriculture-National Institute of Food and Agriculture-Organic Research and Extension Initiative Competitive Grant No. 2010-513000-21760. NR 29 TC 1 Z9 1 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD DEC PY 2015 VL 39 IS 6 BP 2728 EP 2737 DI 10.1111/jfpp.12523 PG 10 WC Food Science & Technology SC Food Science & Technology GA DA9JZ UT WOS:000368126300238 ER PT J AU Helfenstein, J Pawlowski, ML Hill, CB Stewart, J Lagos-Kutz, D Bowen, CR Frossard, E Hartman, GL AF Helfenstein, Julian Pawlowski, Michelle L. Hill, Curtis B. Stewart, Jessica Lagos-Kutz, Doris Bowen, Charles Roger Frossard, Emmanuel Hartman, Glen L. TI Zinc deficiency alters soybean susceptibility to pathogens and pests SO JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE LA English DT Article DE Aphis glycines; Glycine max; Phakopsora pachyrhizi; Sclerotinia sclerotiorum; Xanthomonas axonopodis ID BIOTIC STRESS; PLANTS; RESISTANCE; NUTRIENTS; LEAVES; APHID; REGISTRATION; RESPONSES; NITROGEN; DISEASES AB Inadequate plant nutrition and biotic stress are key threats to current and future crop yields. Zinc (Zn) deficiency and toxicity in major crop plants have been documented, but there is limited information on how pathogen and pest damage may be affected by differing plant Zn levels. In our study, we used soybean plants as a host, a soybean pest, and three soybean pathogens to determine whether plant Zn levels change pest and disease assessments. Two soybean cultivars were grown in sand culture with a soluble nutrient solution that ranged from Zn-deficient to toxic. Detached leaves from these plants were either inoculated with Aphis glycines, the soybean aphid, Xanthomonas axonopodis pv. glycines, a bacterium that causes bacterial pustule, Sclerotinia sclerotiorum, the necrotrophic fungus responsible for stem rot, or Phakopsora pachyrhizi, a biotrophic obligate pathogen that causes soybean rust. There were significant (P < 5%) effects on aphid colonization, positive counts for bacterial pustule, S. sclerotiorum leaf area affected, and numbers of rust lesions associated with the Zn treatments. Plants grown with the physiologically optimal levels of Zn (2 mu M) had less (P < 5%) soybean aphids cm(-2) leaflet than plants grown without Zn, at 0.1 x Zn (0.2 mu M), or at 100x Zn fertilization (200 mu M). Plants grown with the normal fertilization of Zn or 100x Zn had fewer (P < 5%) positive counts for bacterial pustule and less lesion area affected by S. sclerotiorum than plants grown without Zn or fertilized with 0.1x Zn. For soybean rust, plants grown with the physiologically optimal fertilization of Zn or 100x Zn had higher (P < 5%) lesions cm(-2) on leaflets from plants grown without Zn or fertilized with 0.1x Zn. These results indicate different Zn nutrition levels in soybean significantly affected aphid and disease development. C1 [Helfenstein, Julian; Frossard, Emmanuel] ETH, Swiss Fed Inst Technol, Dept Environm Syst Sci, Zurich, Switzerland. [Pawlowski, Michelle L.; Hill, Curtis B.; Stewart, Jessica; Lagos-Kutz, Doris; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Bowen, Charles Roger; Hartman, Glen L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Helfenstein, Julian/0000-0002-5012-2589 NR 36 TC 0 Z9 0 U1 5 U2 14 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1436-8730 EI 1522-2624 J9 J PLANT NUTR SOIL SC JI J. Plant Nutr. Soil Sci. PD DEC PY 2015 VL 178 IS 6 BP 896 EP 903 DI 10.1002/jpln.201500146 PG 8 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA DB3TZ UT WOS:000368436100010 ER PT J AU Arias, RS Dang, PM Sobolev, VS AF Arias, Renee S. Dang, Phat M. Sobolev, Victor S. TI RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 106; RNA interference; silencing; groundnut; seed; transgenic; Aspergillus ID DOUBLE-STRANDED-RNA; HEPATOCELLULAR-CARCINOMA; ASPERGILLUS-FLAVUS; PHYTOALEXIN PRODUCTION; LIQUID-CHROMATOGRAPHY; GENE; PLANTS; EXPOSURE; PATHWAY; CELLS AB The Food and Agriculture Organization of the United Nations estimates that 25% of the food crops in the world are contaminated with aflatoxins. That represents 100 million tons of food being destroyed or diverted to non-human consumption each year. Aflatoxins are powerful carcinogens normally accumulated by the fungi Aspergillus flavus and A. parasiticus in cereals, nuts, root crops and other agricultural products. Silencing of five aflatoxin-synthesis genes by RNA interference (RNAi) in peanut plants was used to control aflatoxin accumulation following inoculation with A. flavus. Previously, no method existed to analyze the effectiveness of RNAi in individual peanut transgenic events, as these usually produce few seeds, and traditional methods of large field experiments under aflatoxin-conducive conditions were not an option. In the field, the probability of finding naturally contaminated seeds is often 1/100 to 1/1,000. In addition, aflatoxin contamination is not uniformly distributed. Our method uses few seeds per transgenic event, with small pieces processed for real-time PCR (RT-PCR) or small RNA sequencing, and for analysis of aflatoxin accumulation by ultra-performance liquid chromatography (UPLC). RNAi-expressing peanut lines 288-72 and 288-74, showed up to 100% reduction (p=0.01) in aflatoxin B-1 and B-2 compared to the control that accumulated up to 14,000 ng g(-1) of aflatoxin B-1 when inoculated with aflatoxigenic A. flavus. As reference, the maximum total of aflatoxins allowable for human consumption in the United States is 20 ng. g-1. This protocol describes the application of RNAi-mediated control of aflatoxins in transgenic peanut seeds and methods for its evaluation. We believe that its application in breeding of peanut and other crops will bring rapid advancement in this important area of science, medicine and human nutrition, and will significantly contribute to the international effort to control aflatoxins, and potentially other mycotoxins in major food crops. C1 [Arias, Renee S.; Dang, Phat M.; Sobolev, Victor S.] ARS, USDA, Natl Peanut Res Lab, Washington, DC 20250 USA. RP Arias, RS (reprint author), ARS, USDA, Natl Peanut Res Lab, Washington, DC 20250 USA. EM renee.arias@ars.usda.gov FU USDA-ARS [6604-21000-004-00D, 6604-42000-008-00D]; USAID Feed-the-Future program [58-0210-3-012] FX This work received the financial support of USDA-ARS CRIS project 6604-21000-004-00D, CRIS project 6604-42000-008-00D, and USAID Feed-the-Future program Agreement number 58-0210-3-012. We thank Valerie Orner, LaTanya Johnson, Joseph Powell and Kathy Gray for their technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 64 TC 0 Z9 0 U1 3 U2 10 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD DEC PY 2015 IS 106 AR e53398 DI 10.3791/53398 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DB5SR UT WOS:000368574400047 PM 26709851 ER PT J AU Hagler, JR Blackmer, F Spurgeon, DW AF Hagler, James R. Blackmer, Felisa Spurgeon, Dale W. TI Accuracy of a prey-specific DNA assay and a generic prey-immunomarking assay for detecting predation SO METHODS IN ECOLOGY AND EVOLUTION LA English DT Article DE Chrysoperla carnea; ELISA; gut analysis; PCR; predator-prey interactions; statistical analysis ID GUT-CONTENT-ANALYSIS; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; BEMISIA-TABACI; IMMUNOLOGICAL APPROACH; SCAR MARKERS; HEMIPTERA; IDENTIFICATION; DETECTABILITY; PARASITOIDS AB 1 Predator gut examinations are useful for detecting arthropod predation events. The accuracy and reproducibility of two different gut assays are tested on various predator species that consumed Chrysoperla carnea (Stephens) that was externally labelled with rabbit immunoglobulin (IgG). 2 Each predator homogenate was examined in triplicate for prey remains by both a conventional PCR assay to detect for C. carnea DNA and a generic ELISA to detect for rabbit IgG-marked prey. The ability of each method to detect predation over time was compared among predators, and between assay types were determined using a novel three-dimensional contingency table approach. 3 Both assays reliably detected prior predation (e.g. at least one of the three subsamples yielded a positive reaction) for 6-12 h after feeding. However, the generic ELISA was more reproducible (e.g. all three subsamples yielded the same outcome) than the PCR. 4 This shows that it was important to assay the predators in triplicate by PCR to avoid a high occurrence of false-negative reactions. Conversely, reproducible results from the ELISA procedure were not dependent on duplicate subsamples. Overall, the generic immunomarking gut assay procedure proved an effective method to assess predation. C1 [Hagler, James R.; Blackmer, Felisa; Spurgeon, Dale W.] USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. RP Hagler, JR (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov FU USDA CRIS [5347-22620-021-00D] FX Funding was provided by USDA CRIS 5347-22620-021-00D. We are grateful for the technical support of Scott Machtley. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 46 TC 2 Z9 2 U1 3 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2041-210X EI 2041-2096 J9 METHODS ECOL EVOL JI Methods Ecol. Evol. PD DEC PY 2015 VL 6 IS 12 BP 1426 EP 1434 DI 10.1111/2041-210X.12436 PG 9 WC Ecology SC Environmental Sciences & Ecology GA DB4XU UT WOS:000368517700006 ER PT J AU Picklo, MJ Long, EK Vomhof-DeKrey, EE AF Picklo, Matthew J. Long, Eric K. Vomhof-DeKrey, Emilie E. TI Glutathionyl systems and metabolic dysfunction in obesity SO NUTRITION REVIEWS LA English DT Review DE obesity; glutathione; glutathionylation; glutathione peroxidase; glutathione S-transferase; insulin; inflammation ID PROTEIN S-GLUTATHIONYLATION; CORONARY-ARTERY-DISEASE; FACTOR-KAPPA-B; ALPHA-KETOGLUTARATE DEHYDROGENASE; TYPE-2 DIABETES SUSCEPTIBILITY; TRANSCRIPTION FACTOR NRF2; OXIDATIVE STRESS SENSOR; DIET-INDUCED OBESITY; ADIPOSE-TISSUE; LIPID-PEROXIDATION AB Oxidative stress is associated with obesity. However, glutathione (GSH), one of the body's most abundant antioxidants, plays dual and seemingly contradictory roles in the development of obesity and its comorbidities. Glutathione has complex metabolic and biochemical fates and is a cofactor for several enzymes that function in modifying obesity-related responses. For example, depletion of GSH increases energy metabolism and reduces adipose accretion, while elevation of GSH peroxidase activity induces insulin resistance. This review summarizes the literature linking GSH and its related enzymes, GSH peroxidase, glutaredoxins, and glutathione S-transferases, to obesity and its pertinent endpoints (e.g., energy metabolism, inflammation, and insulin resistance). C1 [Picklo, Matthew J.; Vomhof-DeKrey, Emilie E.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Picklo, Matthew J.] Univ N Dakota, Dept Chem, Grand Forks, ND 58202 USA. [Long, Eric K.] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA. RP Picklo, MJ (reprint author), USDA ARS, Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. EM matthew.picklo@ars.usda.gov FU USDA-ARS [5450-51000-048-00D] FX Funding was provided through USDA-ARS Project 5450-51000-048-00D. The US Department of Agriculture, Agricultural Research Service, Plains Area, is an equal opportunity/affirmative action employer, and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 151 TC 6 Z9 7 U1 2 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD DEC PY 2015 VL 73 IS 12 BP 858 EP 868 DI 10.1093/nutrit/nuv042 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DB3NC UT WOS:000368417400004 PM 26493322 ER PT J AU Erb, M Robert, CAM Marti, G Lu, J Doyen, GR Villard, N Barriere, Y French, BW Wolfender, JL Turlings, TCJ Gershenzon, J AF Erb, Matthias Robert, Christelle A. M. Marti, Guillaume Lu, Jing Doyen, Gwladys R. Villard, Neil Barriere, Yves French, B. Wade Wolfender, Jean-Luc Turlings, Ted C. J. Gershenzon, Jonathan TI A Physiological and Behavioral Mechanism for Leaf Herbivore-Induced Systemic Root Resistance SO PLANT PHYSIOLOGY LA English DT Article ID WESTERN CORN-ROOTWORM; BELOW-GROUND HERBIVORY; PLANT-MEDIATED INTERACTIONS; P-COUMARIC ACID; CELL-WALL; INTERSPECIFIC INTERACTIONS; PHYTOPHAGOUS INSECTS; FEEDING STIMULANTS; LARVAE COLEOPTERA; DEFENSE RESPONSES AB Indirect plant-mediated interactions between herbivores are important drivers of community composition in terrestrial ecosystems. Among the most striking examples are the strong indirect interactions between spatially separated leaf-and root-feeding insects sharing a host plant. Although leaf feeders generally reduce the performance of root herbivores, little is known about the underlying systemic changes in root physiology and the associated behavioral responses of the root feeders. We investigated the consequences of maize (Zea mays) leaf infestation by Spodoptera littoralis caterpillars for the root-feeding larvae of the beetle Diabrotica virgifera virgifera, a major pest of maize. D. virgifera strongly avoided leaf-infested plants by recognizing systemic changes in soluble root components. The avoidance response occurred within 12 h and was induced by real and mimicked herbivory, but not wounding alone. Roots of leaf-infested plants showed altered patterns in soluble free and soluble conjugated phenolic acids. Biochemical inhibition and genetic manipulation of phenolic acid biosynthesis led to a complete disappearance of the avoidance response of D. virgifera. Furthermore, bioactivity-guided fractionation revealed a direct link between the avoidance response of D. virgifera and changes in soluble conjugated phenolic acids in the roots of leaf-attacked plants. Our study provides a physiological mechanism for a behavioral pattern that explains the negative effect of leaf attack on a root-feeding insect. Furthermore, it opens up the possibility to control D. virgifera in the field by genetically mimicking leaf herbivore-induced changes in root phenylpropanoid patterns. C1 [Erb, Matthias; Robert, Christelle A. M.] Univ Bern, Inst Plant Sci, Altenbergrain 21, CH-3013 Bern, Switzerland. [Erb, Matthias; Robert, Christelle A. M.; Lu, Jing] Max Planck Inst Chem Ecol, Dept Biochem, Root Herbivore Interact Grp, DE-07745 Jena, Germany. [Gershenzon, Jonathan] Max Planck Inst Chem Ecol, Dept Biochem, DE-07745 Jena, Germany. [Erb, Matthias; Robert, Christelle A. M.; Doyen, Gwladys R.; Villard, Neil; Turlings, Ted C. J.] Univ Neuchatel, Lab Fundamental & Appl Res Chem Ecol, CH-2009 Neuchatel, Switzerland. [Marti, Guillaume; Wolfender, Jean-Luc] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, Phytochem & Bioact Nat Prod, CH-1211 Geneva 4, Switzerland. [Barriere, Yves] INRA, Unite Genet & Ameliorat Plantes Fourrageres, F-86600 Lusignan, France. [French, B. Wade] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Erb, M (reprint author), Univ Bern, Inst Plant Sci, Altenbergrain 21, CH-3013 Bern, Switzerland. EM matthias.erb@ips.unibe.ch RI Marti, Guillaume/B-4395-2016; Gershenzon, Jonathan/K-1331-2013; Turlings, Ted/E-8671-2012; OI Marti, Guillaume/0000-0002-6321-9005; Gershenzon, Jonathan/0000-0002-1812-1551; Turlings, Ted/0000-0002-8315-785X; Erb, Matthias/0000-0002-4446-9834 FU Swiss National Science Foundation [FN 107974, FN 152613]; National Centre of Competence in Research "Plant Survival," a research program of the Swiss National Science Foundation; Max Planck Society FX This work was supported by the Swiss National Science Foundation (grant nos. FN 107974 and FN 152613 to M.E., G.R.D., N.V., and T.C.J.T.); the National Centre of Competence in Research "Plant Survival," a research program of the Swiss National Science Foundation; and the Max Planck Society. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal-opportunity provider and employer. NR 51 TC 4 Z9 5 U1 11 U2 44 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD DEC PY 2015 VL 169 IS 4 BP 2884 EP 2894 DI 10.1104/pp.15.00759 PG 11 WC Plant Sciences SC Plant Sciences GA DB4GY UT WOS:000368472700039 PM 26430225 ER PT J AU Perochon, A Jia, JG Kahla, A Arunachalam, C Scofield, SR Bowden, S Wallington, E Doohan, FM AF Perochon, Alexandre Jia Jianguang Kahla, Amal Arunachalam, Chanemougasoundharam Scofield, Steven R. Bowden, Sarah Wallington, Emma Doohan, Fiona M. TI TaFROG Encodes a Pooideae Orphan Protein That Interacts with SnRK1 and Enhances Resistance to the Mycotoxigenic Fungus Fusarium graminearum SO PLANT PHYSIOLOGY LA English DT Article ID HEAD BLIGHT RESISTANCE; DEFENSE RESPONSE GENES; ARABIDOPSIS-THALIANA; TRANSCRIPTOME ANALYSIS; WHEAT SPIKES; KINASE 1; DEOXYNIVALENOL; EXPRESSION; STRESS; TRANSFORMATION AB All genomes encode taxonomically restricted orphan genes, and the vast majority are of unknown function. There is growing evidence that such genes play an important role in the environmental adaptation of taxa. We report the functional characterization of an orphan gene (Triticum aestivum Fusarium Resistance Orphan Gene [TaFROG]) as a component of resistance to the globally important wheat (T. aestivum) disease, Fusarium head blight. TaFROG is taxonomically restricted to the grass subfamily Pooideae. Gene expression studies showed that it is a component of the early wheat response to the mycotoxin deoxynivalenol (DON), which is a virulence factor produced by the causal fungal agent of Fusarium head blight, Fusarium graminearum. The temporal induction of TaFROG by F. graminearum in wheat spikelets correlated with the activation of the defense Triticum aestivum Pathogenesis-Related-1 (TaPR1) gene. But unlike TaPR1, TaFROG induction by F. graminearum was toxin dependent, as determined via comparative analysis of the effects of wild-type fungus and a DON minus mutant derivative. Using virus-induced gene silencing and overexpressing transgenic wheat lines, we present evidence that TaFROG contributes to host resistance to both DON and F. graminearum. TaFROG is an intrinsically disordered protein, and it localized to the nucleus. A wheat alpha subunit of the Sucrose Non-Fermenting1-Related Kinase1 was identified as a TaFROG-interacting protein based on a yeast two-hybrid study. In planta bimolecular fluorescence complementation assays confirmed the interaction. Thus, we conclude that TaFROG encodes a new Sucrose Non-Fermenting1-Related Kinase1-interacting protein and enhances biotic stress resistance. C1 [Perochon, Alexandre; Jia Jianguang; Kahla, Amal; Arunachalam, Chanemougasoundharam; Doohan, Fiona M.] Univ Coll Dublin, Earth Inst, Dublin 4, Ireland. [Perochon, Alexandre; Jia Jianguang; Kahla, Amal; Arunachalam, Chanemougasoundharam; Doohan, Fiona M.] Univ Coll Dublin, Coll Sci, Sch Biol & Environm Sci, Dublin 4, Ireland. [Scofield, Steven R.] ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47904 USA. [Scofield, Steven R.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Bowden, Sarah; Wallington, Emma] Natl Inst Agr Bot, Cambridge CB3 0LE, England. RP Doohan, FM (reprint author), Univ Coll Dublin, Earth Inst, Dublin 4, Ireland. EM fiona.doohan@ucd.ie RI Scofield, Steven/C-3868-2016 FU Science Foundation Ireland [10/IN.1/B3028, 14/1A/2508]; National Institute of Agricultural Botany Trust FX This work was supported by the Science Foundation Ireland (project nos. 10/IN.1/B3028 and 14/1A/2508) and the National Institute of Agricultural Botany Trust. The authors have a patent pending related to this material. NR 67 TC 2 Z9 2 U1 6 U2 14 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD DEC PY 2015 VL 169 IS 4 BP 2895 EP 2906 DI 10.1104/pp.15.01056 PG 12 WC Plant Sciences SC Plant Sciences GA DB4GY UT WOS:000368472700040 PM 26508775 ER PT J AU Folta, SC Koomas, A Metayer, N Fullerton, KJ Hubbard, KL Anzman-Frasca, S Hofer, T Nelson, M Newman, M Sacheck, J Economos, C AF Folta, Sara C. Koomas, Alyssa Metayer, Nesly Fullerton, Karen J. Hubbard, Kristie L. Anzman-Frasca, Stephanie Hofer, Teresa Nelson, Miriam Newman, Molly Sacheck, Jennifer Economos, Christina TI Engaging Stakeholders From Volunteer-Led Out-of-School Time Programs in the Dissemination of Guiding Principles for Healthy Snacking and Physical Activity SO PREVENTING CHRONIC DISEASE LA English DT Article AB Background Little effort has focused on the role of volunteer-led out-of-school time (OST) programs (ie, enrichment and sports programs) as key environments for the promotion of healthy eating and physical activity habits among school-aged children. The Healthy Kids Out of School (HKOS) initiative developed evidence-based, practical guiding principles for healthy snacks, beverages, and physical activity. The goal of this case study was to describe the methods used to engage regional partners to understand how successful implementation and dissemination of these principles could be accomplished. Community Context HKOS partnered with volunteer-led programs from 5 OST organizations in Maine, Massachusetts, and New Hampshire to create a regional "learning laboratory." Methods We engaged partners in phases. In the first phase, we conducted focus groups with local volunteer program leaders; during the second phase, we held roundtable meetings with regional and state program administrators; and in the final phase, we conducted additional outreach to refine and finalize implementation strategies. Outcomes Implementation strategies were developed based on themes and information that emerged. For enrichment programs, strategies included new patch and pin programs that were consistent with the organizations' infrastructure and usual practices. For sports programs, the main strategy was integration with online trainings for coaches. Interpretation Through the engagement process, we learned that dissemination of the guiding principles in these large and complex OST organizations was best accomplished by using implementation strategies that were customized, integrated, and aligned with goals and usual practices. The lessons learned can benefit future efforts to prevent obesity in complex environments. C1 [Folta, Sara C.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Koomas, Alyssa; Metayer, Nesly; Fullerton, Karen J.; Anzman-Frasca, Stephanie; Nelson, Miriam; Sacheck, Jennifer; Economos, Christina] Tufts Univ, Boston, MA 02111 USA. [Hubbard, Kristie L.] USDA, Food & Nutr Serv, Western Reg Off, San Francisco, CA USA. [Hofer, Teresa] City Hope Natl Med Ctr, Monrovia, CA USA. RP Folta, SC (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Boston, MA 02111 USA. EM sara.folta@tufts.edu FU Harvard Pilgrim Health Care Foundation; Robert Wood Johnson Foundation; JPB Foundation FX Regional support for the HKOS initiative in Maine, Massachusetts, and New Hampshire is provided by the program's core funder, the Harvard Pilgrim Health Care Foundation. Strategic funding for ChildObesity180 is provided by the Robert Wood Johnson Foundation and The JPB Foundation. We thank the volunteer program leaders and program administrators for their willingness to engage in this process. We also acknowledge Rebecca Rottapel and Julianne Heck for assistance with manuscript editing prior to submission. NR 17 TC 0 Z9 0 U1 2 U2 6 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD DEC PY 2015 VL 12 AR 150270 DI 10.5888/pcd12.150270 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DB7AC UT WOS:000368665800015 ER PT J AU Powell, MR AF Powell, Mark R. TI Risk-Based Sampling: I Don't Want to Weight in Vain SO RISK ANALYSIS LA English DT Article DE Risk-based sampling; sanitary and phytosanitary risk ID VARIANCE-EFFICIENT PORTFOLIOS; NAIVE DIVERSIFICATION; UNCERTAIN MEANS; COVARIANCES; SELECTION; CHOICE; STRATEGIES AB Recently, there has been considerable interest in developing risk-based sampling for food safety and animal and plant health for efficient allocation of inspection and surveillance resources. The problem of risk-based sampling allocation presents a challenge similar to financial portfolio analysis. Markowitz (1952) laid the foundation for modern portfolio theory based on mean-variance optimization. However, a persistent challenge in implementing portfolio optimization is the problem of estimation error, leading to false "optimal" portfolios and unstable asset weights. In some cases, portfolio diversification based on simple heuristics (e.g., equal allocation) has better out-of-sample performance than complex portfolio optimization methods due to estimation uncertainty. Even for portfolios with a modest number of assets, the estimation window required for true optimization may imply an implausibly long stationary period. The implications for risk-based sampling are illustrated by a simple simulation model of lot inspection for a small, heterogeneous group of producers. C1 [Powell, Mark R.] USDA, Off Risk Assessment & Cost Benefit Anal, Washington, DC 20250 USA. RP Powell, MR (reprint author), USDA, Off Risk Assessment & Cost Benefit Anal, 1400 Independence Ave SW,MS 3811, Washington, DC 20250 USA. EM mpowell@oce.usda.gov NR 70 TC 0 Z9 0 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD DEC PY 2015 VL 35 IS 12 BP 2172 EP 2182 DI 10.1111/risa.12415 PG 11 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DB2MW UT WOS:000368344000007 PM 26033352 ER PT J AU Biederman, JA Somor, AJ Harpold, AA Gutmann, ED Breshears, DD Troch, PA Gochis, DJ Scott, RL Meddens, AJH Brooks, PD AF Biederman, Joel A. Somor, Andrew J. Harpold, Adrian A. Gutmann, Ethan D. Breshears, David D. Troch, Peter A. Gochis, David J. Scott, Russell L. Meddens, Arjan J. H. Brooks, Paul D. TI Recent tree die-off has little effect on streamflow in contrast to expected increases from historical studies SO WATER RESOURCES RESEARCH LA English DT Article ID LODGEPOLE PINE FORESTS; WESTERN UNITED-STATES; BRITISH-COLUMBIA; WATER YIELD; SNOW ACCUMULATION; BEETLE INFESTATION; ROCKY-MOUNTAINS; TIMBER HARVEST; NEW-MEXICO; MORTALITY AB Recent bark beetle epidemics have caused regional-scale tree mortality in many snowmelt-dominated headwater catchments of western North America. Initial expectations of increased streamflow have not been supported by observations, and the basin-scale response of annual streamflow is largely unknown. Here we quantified annual streamflow responses during the decade following tree die-off in eight infested catchments in the Colorado River headwaters and one nearby control catchment. We employed three alternative empirical methods: (i) double-mass comparison between impacted and control catchments, (ii) runoff ratio comparison before and after die-off, and (iii) time-trend analysis using climate-driven linear models. In contrast to streamflow increases predicted by historical paired catchment studies and recent modeling, we did not detect streamflow changes in most basins following die-off, while one basin consistently showed decreased streamflow. The three analysis methods produced generally consistent results, with time-trend analysis showing precipitation was the strongest predictor of streamflow variability (R2574-96%). Time-trend analysis revealed post-die-off streamflow decreased in three catchments by 11-29%, with no change in the other five catchments. Although counter to initial expectations, these results are consistent with increased transpiration by surviving vegetation and the growing body of literature documenting increased snow sublimation and evaporation from the subcanopy following die-off in water-limited, snow-dominated forests. The observations presented here challenge the widespread expectation that streamflow will increase following beetle-induced forest die-off and highlight the need to better understand the processes driving hydrologic response to forest disturbance. C1 [Biederman, Joel A.; Scott, Russell L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [Somor, Andrew J.; Troch, Peter A.] Univ Arizona, Dept Hydrol & Water Resources, Tucson, AZ 85721 USA. [Harpold, Adrian A.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Gutmann, Ethan D.; Gochis, David J.] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Breshears, David D.] Univ Arizona, Dept Ecol & Evolut Biol, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Meddens, Arjan J. H.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Brooks, Paul D.] Univ Utah, Dept Geol & Geophys, Salt Lake City, UT 84112 USA. RP Biederman, JA (reprint author), USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. EM joel.biederman@ars.usda.gov RI Gutmann, Ethan/I-5728-2012; OI Gutmann, Ethan/0000-0003-4077-3430; Harpold, Adrian/0000-0002-2566-9574 FU National Science Foundation (NSF) [EAR9876800, EAR0910831, EAR0724958, EAR1144894, AGS-0753581]; USDA NIFA [NEV05293]; DOE TCC [10-287] FX All of the data sets used herein were downloaded from publicly available sources. The gridded temperature forcing data set [Livneh et al., 2013] is available at ftp://ftp.hydro.washington.edu/pub/blivneh/CONUS/. SNOTEL data are available at http://www.wcc.nrcs.usda.gov/snow for the six stations used: 335, 415, 485, 505, 802, and 842. Streamflow data are available at http://waterdata.usgs.gov/nwis for the gauge numbers listed in Table 1. Land cover data for the Southwest Regional Gap Analysis are available at http://earth.gis.usu.edu/swgap. Catchment boundaries are available from www.horizon-systems.com/nhdplus. Aerial survey forest health data are available from http://www.fs.usda.gov/detail/r2/forest-grasslandhealth/?cid5fsbdev3_041 629. The authors wish to thank those individuals and organizations who collected and made these data available. This work was supported by the following awards: NSF EAR9876800, NSF EAR0910831, NSF EAR0724958, and DOE TCC 10-287. A. Harpold was supported by NSF EAR1144894 and USDA NIFA NEV05293. The National Center for Atmospheric Research is sponsored by the National Science Foundation (NSF AGS-0753581). We thank Younes Alila and three anonymous reviewers for their thoughtful comments. NR 78 TC 11 Z9 11 U1 6 U2 25 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD DEC PY 2015 VL 51 IS 12 BP 9775 EP 9789 DI 10.1002/2015WR017401 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DB3OR UT WOS:000368421500022 ER PT J AU Twedt, DJ Linz, GM AF Twedt, Daniel J. Linz, George M. TI FLIGHT FEATHER MOLT IN YELLOW-HEADED BLACKBIRDS (XANTHOCEPHALUS XANTHOCEPHALUS) IN NORTH DAKOTA SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article DE blackbirds; crops; feathers; Icteridae; molt; North Dakota; Xanthocephalus xanthocephalus; Yellow-headed Blackbird ID RED-WINGED BLACKBIRDS; 1ST BASIC PROBLEM; GROWTH AB Yellow-headed Blackbirds (Xanthocephalus xanthocephalus) in central North Dakota undergo prebasic molt or prejuvenile molt during late summer. Nestling Yellow-headed Blackbirds initiate a complete prejuvenile molt, grow their primary and secondary regimes in about 40 days, completing molt after they leave the nest by the first week in August. Remiges are not replaced during the subsequent preformative molt, being retained until the second prebasic molt. Nonlinear (logistic) regression of primary remex growth during definitive prebasic molts of Yellow-headed Blackbirds indicated 38 days were required to complete the linear phase of growth (between 10% and 90% of total primary length). Males added 19.5 mm/d and females added 15.7 mm/d to the total length of all primaries during this linear growth phase; an average of 4-5 mm per primary remex per day. Definitive prebasic molting of primary remiges in males and females was initiated in late June, after nesting and brood rearing were completed. Molts of Yellow-headed Blackbirds were completed by early September, before birds emigrated from North Dakota during mid-September. Because of their comparatively early completion of molt and emigration from the state, as well as their more diverse diet, agricultural depredation caused by Yellow-headed Blackbirds in North Dakota is likely less than that of Red-winged Blackbirds and Common Grackles. C1 [Twedt, Daniel J.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58105 USA. [Linz, George M.] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. RP Twedt, DJ (reprint author), Univ Memphis, US Geol Survey, Patuxent Wildlife Res Ctr, South Campus,950 Getwell Rd, Memphis, TN 38152 USA. EM dtwedt@usgs.gov OI Twedt, Daniel/0000-0003-1223-5045 FU National Wildlife Research Center; USDA-APHIS; Colorado Cooperative Fish and Wildlife Research Unit; Colorado State University; North Dakota State University FX We thank B. Osborne and W. Bleier for providing invaluable field and lab assistance. J. Lindlauf, J. L. Cummings, J. E. Davis, and C. E. Knittle provided additional assistance in the lab. S. Bergrud graciously allowed us to place decoy traps on his property. We thank the numerous landowners who granted access to their fields and wetlands which greatly facilitated our study. The assistance of state and federal wildlife agencies was greatly appreciated. W. Bleier, G. Nuechterlein, F. Mallet-Rodrigues, L Moulton, B. Peer, and K. Yasukawa reviewed a draft of this manuscript. The Denver Wildlife Research Center (now National Wildlife Research Center), USDA-APHIS; Colorado Cooperative Fish and Wildlife Research Unit, Colorado State University; and North Dakota State University provided financial support. NR 37 TC 0 Z9 0 U1 0 U2 0 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 EI 1938-5447 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD DEC PY 2015 VL 127 IS 4 BP 622 EP 629 PG 8 WC Ornithology SC Zoology GA DB2LL UT WOS:000368340000006 ER PT J AU Niederwerder, MC Bawa, B Serao, NVL Trible, BR Kerrigan, MA Lunney, JK Dekkers, JCM Rowland, RRR AF Niederwerder, Megan C. Bawa, Bhupinder Serao, Nick V. L. Trible, Benjamin R. Kerrigan, Maureen A. Lunney, Joan K. Dekkers, Jack C. M. Rowland, Raymond R. R. TI Vaccination with a Porcine Reproductive and Respiratory Syndrome (PRRS) Modified Live Virus Vaccine Followed by Challenge with PRRS Virus and Porcine Circovirus Type 2 (PCV2) Protects against PRRS but Enhances PCV2 Replication and Pathogenesis Compared to Results for Nonvaccinated Cochallenged Controls SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID MULTISYSTEMIC WASTING SYNDROME; NONSTRUCTURAL PROTEIN-1 NSP1; COLOSTRUM-DEPRIVED PIGLETS; CONVENTIONAL PIGS; EXPERIMENTAL-INFECTION; GNOTOBIOTIC SWINE; DISEASE; PMWS; RECOGNITION; PARVOVIRUS AB Coinfections involving porcine reproductive and respiratory syndrome virus ( PRRSV) and porcine circovirus type 2 (PCV2) contribute to a group of disease syndromes known as porcine circovirus-associated disease (PCVAD). Presumably, PRRSV infection enhances PCV2 replication as a result of modulation of host immunity. The purpose of this study was to evaluate PCV2 replication and pathogenesis in pigs vaccinated with a PRRS modified live virus (MLV) vaccine and subsequently challenged with a combination of PRRSV and PCV2. During the early postchallenge period, the number of pigs with PRRSV-associated clinical signs was decreased, and average daily gain (ADG) was increased, in the vaccinated group, demonstrating the protective effect of PRRS vaccination. However, during the later postchallenge period, more pigs in the vaccinated group showed increased PCV2 viremia, decreased ADG, increased PCVAD clinical signs, and increased mortality. In this disease model, the early benefits of PRRSV vaccination were outweighed by the later amplification of PCVAD. C1 [Niederwerder, Megan C.; Bawa, Bhupinder; Trible, Benjamin R.; Kerrigan, Maureen A.; Rowland, Raymond R. R.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Serao, Nick V. L.; Dekkers, Jack C. M.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Lunney, Joan K.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD USA. RP Niederwerder, MC (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. EM mniederwerder@vet.k-state.edu FU USDA NIFA award [2013-68004-20362] FX This work was supported by USDA NIFA award 2013-68004-20362. NR 55 TC 3 Z9 3 U1 2 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 EI 1556-679X J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD DEC PY 2015 VL 22 IS 12 BP 1244 EP 1254 DI 10.1128/CVI.00434-15 PG 11 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DA8EI UT WOS:000368037500004 PM 26446422 ER PT J AU Williams, MM Bradley, CA Duke, SO Maul, JE Reddy, KN AF Williams, Martin M., II Bradley, Carl A. Duke, Stephen O. Maul, Jude E. Reddy, Krishna N. TI Goss's Wilt Incidence in Sweet Corn Is Independent of Transgenic Traits and Glyphosate SO HORTSCIENCE LA English DT Article DE Clavibacter michiganensis ssp nebraskensis; cp4 epsp; cry3 Bb1; weed management; Zea mays ID MICHIGANENSIS SUBSP NEBRASKENSIS; LEAF-BLIGHT; RESISTANT CROPS; BACTERIAL WILT; 1ST REPORT; DISEASE; HERBICIDE; BENEFITS; HYBRIDS AB Recently, claims have been made that the use of glyphosate and transgenic crop traits increases plant susceptibility to pathogens. Transgenic traits used widely for years in dent corn are now available in commercial sweet corn cultivars, specifically, the combination of glyphosate resistance (GR) and Lepidoptera control (Bt). The objective was to assess the interactions of the GR+Bt trait, glyphosate, and Goss's wilt on sweet corn. Nine treatments were tested under weed-free conditions at two sites in 2013 and 2014. Treatments included two isogenic cultivars differing only in the presence or absence of GR+Bt, with and without postemergence application of glyphosate, and inoculation with the causal agent of Goss's wilt (Clavibacter michiganensis ssp. nebraskensis) before glyphosate application, after glyphosate application, or no inoculation. Results failed to show glyphosate or the GR+Bt trait influenced sweet corn susceptibility to Goss's wilt. The only factor affecting Goss's wilt incidence was whether plants were inoculated with C. michiganensis ssp. nebraskensis. In the absence of glyphosate application, yet under weed-free conditions, several yield traits were higher in sweet corn with the GR+Bt trait. Results showed that the GR transgene confers the same level of tolerance to glyphosate in sweet corn as observed previously in dent corn. If true, recent claims about glyphosate and transgenic traits increasing plant disease would be of major concern in sweet corn; however, no relationships were found between the GR+Bt trait and/or glyphosate to Goss's wilt incidence in sweet corn. C1 [Williams, Martin M., II] USDA ARS, Global Change & Photosynthesis Res, Urbana, IL 61801 USA. [Bradley, Carl A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Duke, Stephen O.] USDA ARS, Nat Prod Utilizat Res, University, MS 38677 USA. [Maul, Jude E.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Reddy, Krishna N.] USDA ARS, Crop Prod Syst Res, Stoneville, MS 38776 USA. RP Williams, MM (reprint author), USDA ARS, Global Change & Photosynthesis Res, 1102 South Goodwin Ave, Urbana, IL 61801 USA. EM martin.williams@ars.usda.gov NR 29 TC 2 Z9 2 U1 2 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2015 VL 50 IS 12 BP 1791 EP 1794 PG 4 WC Horticulture SC Agriculture GA DB2BX UT WOS:000368314400008 ER PT J AU Kou, LP Yang, TB Liu, XJ Luo, YG AF Kou, Liping Yang, Tianbao Liu, Xianjin Luo, Yaguang TI Effects of Pre-and Postharvest Calcium Treatments on Shelf Life and Postharvest Quality of Broccoli Microgreens SO HORTSCIENCE LA English DT Article DE broccoli microgreens; calcium chloride; calcium lactate; calcium amino acid; quality; shelf life ID MICROBIAL-POPULATION; APPLE FRUIT; BROWN-ROT; STORAGE; ATTRIBUTES; STABILITY; SENESCENCE; PARAMETERS; CHLORINE; LACTATE AB We reported previously that the preharvest treatment of broccoli microgreens with 10 mmol.L-1 calcium chloride (CaCl2) increased the yield and postharvest quality. The objective of this study was to investigate whether other calcium forms have the similar effect, in particular, after postharvest dip in calcium solution. Our results are as follows: 1) Preharvest spray without postharvest dip: Both 20 mmol.L-1 calcium lactate (Ca lactate) and calcium amino acid (Ca AA) chelate significantly improved broccoli microgreens quality and inhibited microbial populations as compared with the water-only control during storage at 5 degrees C for 21 days. However, they were less effective than 10 mmol.L-1 CaCl2. 2) Postharvest dip without preharvest spray: The microgreens sprayed with water-only control were dipped in 0, 25, 50, or 100 mmol.L-1 Ca lactate solution containing 100 mu L.L-1 chlorine immediately after harvest. During storage at 5 degrees C for 14 days, 50 mmol.L-1 Ca lactate dip showed the highest overall quality and lowest tissue electrolyte leakage. 3) Preharvest spray and postharvest dip: Combined preharvest 10 mmol.L-1 CaCl2 spray and postharvest 50 mmol.L-1 Ca lactate dip resulted in better postharvest quality than individual pre- or postharvest calcium treatments. However, the preharvest 10 mmol.L-1 CaCl2 spray without postharvest dip displayed a best overall visual quality and longest storage life. Our data indicate that pre- and postharvest calcium treatments have positive effect on maintaining the microgreens quality and extending shelf life. However, current postharvest dip/spinning/drying method profoundly reduces the shelf life due to mechanical damages. Technologies to optimize microgreens wash are needed to provide ready-to-eat product. Alternatively, the wash step can be avoided when the microgreens are grown under controlled settings. C1 [Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Yang, Tianbao] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Liu, Xianjin] Jiangsu Acad Agr Sci, Inst Food Qual & Safety, Nanjing 210014, Jiangsu, Peoples R China. [Luo, Yaguang] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. RP Yang, TB (reprint author), USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. EM tianbao.yang@ars.usda.gov NR 37 TC 1 Z9 1 U1 4 U2 21 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2015 VL 50 IS 12 BP 1801 EP 1808 PG 8 WC Horticulture SC Agriculture GA DB2BX UT WOS:000368314400010 ER PT J AU Ehlenfeldt, MK Martin, RB Rowland, LJ AF Ehlenfeldt, Mark K. Martin, Robert B. Rowland, Lisa J. TI 'Nocturne' Hybrid Blueberry: A Winter-hardy, Mixed-species Hexaploid with Ornamental Landscape Interest and Novel Fruit Quality SO HORTSCIENCE LA English DT Editorial Material DE cold hardiness; rabbiteye; Vaccinium ashei; Vaccinium constablaei; Vaccinium virgatum ID COLD-HARDINESS C1 [Ehlenfeldt, Mark K.; Martin, Robert B.] ARS, Marucci Ctr Blueberry & Cranberry Res & Extens, USDA, Chatsworth, NJ 08019 USA. [Ehlenfeldt, Mark K.; Martin, Robert B.; Rowland, Lisa J.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. RP Ehlenfeldt, MK (reprint author), ARS, Marucci Ctr Blueberry & Cranberry Res & Extens, USDA, Chatsworth, NJ 08019 USA. EM mark.ehlenfeldt@ars.usda.gov NR 9 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2015 VL 50 IS 12 BP 1825 EP 1827 PG 3 WC Horticulture SC Agriculture GA DB2BX UT WOS:000368314400015 ER PT J AU Finn, CE Strik, BC Mackey, TA Hummer, KE Martin, RR AF Finn, Chad E. Strik, Bernadine C. Mackey, Theodore A. Hummer, Kim E. Martin, Robert R. TI 'Perpetua' Ornamental Reflowering Blueberry SO HORTSCIENCE LA English DT Editorial Material DE remontant; off-season flowering; perpetual flowering; Vaccinium corymbosum; Vacciniunz angustifolium C1 [Finn, Chad E.; Mackey, Theodore A.; Martin, Robert R.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Hummer, Kim E.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97330 USA. RP Finn, CE (reprint author), USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. EM Chad.Finn@ARS.USDA.GOV NR 9 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2015 VL 50 IS 12 BP 1828 EP 1829 PG 2 WC Horticulture SC Agriculture GA DB2BX UT WOS:000368314400016 ER PT J AU Rinehart, T Shockey, J Edwards, N Spiers, JM Klasson, T AF Rinehart, Timothy Shockey, Jay Edwards, Ned Spiers, James M. Klasson, Thomas TI Vernicia fordii 'Spiers', a New Tung Tree for Commercial Tung Oil Production in the Gulf Coast Region SO HORTSCIENCE LA English DT Editorial Material DE Aleurites fordii; tung oil tree; tung nut ID SUBDOMAINS C1 [Rinehart, Timothy; Edwards, Ned; Spiers, James M.] USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Shockey, Jay; Klasson, Thomas] USDA ARS, Commod Utilizat Res Unit, New Orleans, LA 70124 USA. RP Rinehart, T (reprint author), USDA ARS, Thad Cochran Southern Hort Lab, 810 Highway 26 West, Poplarville, MS 39470 USA. EM Tim.Rinehart@ars.usda.gov NR 10 TC 0 Z9 0 U1 2 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2015 VL 50 IS 12 BP 1830 EP 1832 PG 3 WC Horticulture SC Agriculture GA DB2BX UT WOS:000368314400017 ER PT J AU Charnley, S Poe, MR Ager, AA Spies, TA Platt, EK Olsen, KA AF Charnley, Susan Poe, Melissa R. Ager, Alan A. Spies, Thomas A. Platt, Emily K. Olsen, Keith A. TI A Burning Problem: Social Dynamics of Disaster Risk Reduction through Wildfire Mitigation SO HUMAN ORGANIZATION LA English DT Article DE natural hazard mitigation; Forest Service; United States ID WILDLAND-URBAN INTERFACE; UNITED-STATES; INLAND NORTHWEST; FUEL TREATMENTS; MANAGEMENT; FORESTS; VULNERABILITY; LANDSCAPES; HAZARDS; POLICY AB Disasters result from hazards affecting vulnerable people. Most disasters research by anthropologists focuses on vulnerability; this article focuses on natural hazards. We use the case of wildfire mitigation on United States Forest Service lands in the northwestern United States to examine social, political, and economic variables at multiple scales that influence fire hazard and risk reduction treatments and their effectiveness. Variables highlighted include policy direction to prioritize wildfire risk reduction in the wildland-urban interface, laws and policies that make treating fuels in some national forest land management allocations challenging, social and political constraints on using prescribed fire, agency budget and target pressures, and integrating fire hazard reduction into forest management projects having multiple objectives. These variables compromise the effectiveness of wildfire mitigation treatments. Understanding the social dynamics of natural hazard mitigation is important because they affect its outcomes, creating differential exposure to natural hazards-one component of social vulnerability. Interdisciplinary research to identify how the social dynamics of natural hazard mitigation influence hazard reduction outcomes can contribute to more informed and effective approaches to disaster risk reduction. C1 [Charnley, Susan; Ager, Alan A.; Spies, Thomas A.] US Forest Serv, Pacific NW Res Stn, Washington, DC 20252 USA. [Poe, Melissa R.] Univ Washington, Sea Grant Program, Seattle, WA 98195 USA. [Poe, Melissa R.] NOAA, Northwest Fisheries Sci tr, Silver Spring, MD USA. [Platt, Emily K.] US Forest Serv, Reg 6, Washington, DC USA. [Olsen, Keith A.] Oregon State Univ, Corvallis, OR 97331 USA. RP Charnley, S (reprint author), US Forest Serv, Pacific NW Res Stn, Washington, DC 20252 USA. FU National Science Foundation; Coupled Human and Natural Systems Program Grant [CHH-1013296]; National Fire Plan from the United States Forest Service FX Susan Charnley is a Research Social Scientist with the United States Forest Service's Pacific Northwest Research Station. Melissa R. Poe is an Environmental Social Scientist with the University of Washington Sea Grant Program and liaison with NOAA's Northwest Fisheries Science Center. Alan A. Ager is an Operations Research Analyst with the United States Forest Service's Pacific Northwest Research Station. Thomas A. Spies is a Senior Scientist with the United States Forest Service's Pacific Northwest Research Station. Emily K. Platt is a Planning Specialist with United States Forest Service Region 6. Keith A. Olsen is a Geographic Information Systems specialist at Oregon State University. This research was supported by a National Science Foundation, Coupled Human and Natural Systems Program Grant (CHH-1013296) and National Fire Plan funding from the United States Forest Service. The authors thank Kendra Wendel who provided essential help with data analysis and manuscript preparation. A.J. Faas, Roberto Barrios, and three anonymous reviewers provided excellent comments that greatly improved the article. NR 51 TC 3 Z9 3 U1 4 U2 16 PU SOC APPLIED ANTHROPOLOGY PI OKLAHOMA CITY PA 3000 UNITED FOUNDERS BLVD, STE 148, OKLAHOMA CITY, OK 73112 USA SN 0018-7259 EI 1938-3525 J9 HUM ORGAN JI Hum. Organ. PD WIN PY 2015 VL 74 IS 4 BP 329 EP 340 PG 12 WC Anthropology; Social Sciences, Interdisciplinary SC Anthropology; Social Sciences - Other Topics GA DA5WR UT WOS:000367873800005 ER PT J AU Schemerhorn, BJ Crane, YM Crane, CF AF Schemerhorn, Brandon J. Crane, Yan Ma Crane, Charles F. TI The evolution of Hessian fly from the Old World to the New World: Evidence from molecular markers SO INSECT SCIENCE LA English DT Article DE allele frequency; Hessian fly; microsatellite; population structure; SNP; SSR ID SOUTHEASTERN UNITED-STATES; DIPTERA CECIDOMYIIDAE; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; SOFTWARE; WHEAT; DNA; POLYMORPHISMS; LENGTH AB Eighteen polymorphic microsatellite loci and 11 single-nucleotide polymorphisms were genotyped in 1 095 individual Hessian fly specimens representing 23 populations from North America, southern Europe, and southwest Asia. The genotypes were used to assess genetic diversity and interrelationship of Hessian fly populations. While phylogenetic analysis indicates that the American populations most similar to Eurasian populations come from the east coast of the United States, genetic distance is least between (Alabama and California) and (Kazakhstan and Spain). Allelic diversity and frequency vary across North America, but they are not correlated with distance from the historically documented point of introduction in New York City or with temperature or precipitation. Instead, the greatest allelic diversity mostly occurs in areas with Mediterranean climates. The microsatellite data indicate a general deficiency for heterozygotes in Hessian fly. The North American population structure is consistent with multiple introductions, isolation by distance, and human-abetted dispersal by bulk transport of puparia in infested straw or on harvesting equipment. C1 [Schemerhorn, Brandon J.; Crane, Yan Ma; Crane, Charles F.] Purdue Univ, USDA ARS, W Lafayette, IN 47907 USA. [Schemerhorn, Brandon J.; Crane, Yan Ma] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Crane, Charles F.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. RP Schemerhorn, BJ (reprint author), Purdue Univ, USDA ARS, Dept Entomol, W Lafayette, IN 47906 USA. EM bschemer@purdue.edu FU USDA-ARS CRIS [3602-22000-1] FX We would like to thank Sue Cambron and Richard Smith for greenhouse assistance. This work was supported by USDA-ARS CRIS 3602-22000-1. NR 37 TC 1 Z9 1 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD DEC PY 2015 VL 22 IS 6 BP 768 EP 784 DI 10.1111/1744-7917.12175 PG 17 WC Entomology SC Entomology GA DB1LR UT WOS:000368270200007 PM 25263747 ER PT J AU Pawlowski, M Hill, CB Voegtlin, DJ Hartman, GL AF Pawlowski, Michelle Hill, Curtis B. Voegtlin, David J. Hartman, Glen L. TI Soybean aphid intrabiotype variability based on colonization of specific soybean genotypes SO INSECT SCIENCE LA English DT Article DE Aphis glycines; biotype; biotype variant; soybean; soybean aphid resistance ID SINGLE DOMINANT GENE; INSECT BIOTYPES; RESISTANCE; HOMOPTERA; EVOLUTIONARY; INHERITANCE; DISCOVERY; GERMPLASM; RESPONSES; CLONES AB The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most destructive insect pests on soybeans in the United States. One method for managing this pest is through host plant resistance. Since its arrival in 2000, 4 aphid biotypes have been identified that are able to overcome soybean aphid resistance (Rag) genes. A soybean aphid isolate collected from Moline, Illinois readily colonized soybean plants with the soybean aphid resistance gene Rag2, unlike biotypes 1 and 2, but similar to soybean aphid biotype 3. Two no-choice experiments compared the virulence of the Moline isolate with biotype 3. In both experiments, differences in aphid population counts were not significant (P > 0.05) on soybean genotypes LD08-12957a (Rag2) and LD11-5413a (Rag2), but the aphid counts for the Moline isolate were significantly (P < 0.05) lower than the aphid counts for the biotype 3 isolate on the soybean genotypes Dowling (Rag1), LD05-16611 (Rag1), LD11-4576a (Rag1), and PI 567598B (rag1b and rag3). The Moline isolate was a variant of aphid biotype 3, which is the first report showing that soybean aphid isolates classified as the same biotype, based on virulence against specific Rag genes, can differ in aggressiveness or ability to colonize specific host genotypes. C1 [Pawlowski, Michelle; Hill, Curtis B.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Natl Soybean Res Ctr, Urbana, IL 61801 USA. [Voegtlin, David J.] Univ Illinois, Illinois Nat Hist Survey, Ctr Econ Entomol, Champaign, IL 61820 USA. [Hartman, Glen L.] Univ Illinois, USDA ARS, Natl Soybean Res Ctr, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Natl Soybean Res Ctr, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU United Soybean Board FX We are grateful for the help of Anitha Chirumamilla, Theresa Herman, Lauren Johnson, and Nicole Westfall for aiding in conducting and rating experiments, and to the United Soybean Board for providing funds for this research. NR 41 TC 1 Z9 1 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD DEC PY 2015 VL 22 IS 6 BP 785 EP 792 DI 10.1111/1744-7917.12169 PG 8 WC Entomology SC Entomology GA DB1LR UT WOS:000368270200008 PM 25183413 ER PT J AU Woltz, JM Donahue, KM Bruck, DJ Lee, JC AF Woltz, J. M. Donahue, K. M. Bruck, D. J. Lee, J. C. TI Efficacy of commercially available predators, nematodes and fungal entomopathogens for augmentative control of Drosophila suzukii SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE biocontrol; Drosophila suzukii; entomopathogenic fungi; entomopathogenic nematodes; Metarhizium anisopliae; Orius insidiosus ID MATSUMURA DIPTERA DROSOPHILIDAE; SPOTTED WING DROSOPHILA; INTEGRATED PEST-MANAGEMENT; BIOLOGICAL-CONTROL AGENTS; LARVAL PARASITOIDS; NATURAL ENEMIES; ATHETA-CORIARIA; UNITED-STATES; INSECTICIDES; PROGRAMS AB The recent arrival of Drosophila suzukii, an invasive pest of soft-skinned fruit with a wide host range, has resulted in increased production costs for growers and the need for additional insecticide applications each growing season. There are few effective organic insecticides for D. suzukii, and insecticide use in conventional farms may be disruptive to natural enemies, suggesting a need for effective biological control to combat D. suzukii. Commercially available natural enemies were evaluated for their potential use in augmentative releases, including: the predators Orius insidiosus and Dalotia coriaria; the entomopathogenic fungi Metarhizium anisopliae, Beauveria bassiana and Paecilomyces fumosoroseus; and the entomopathogenic nematodes Heterorhabditis bacteriophora, Steinernema feltiae and S. carpocapsae. This suite of natural enemies was chosen to target D. suzukii adults as well as larvae in hanging or dropped fruit. Of the cultured fungal strains tested, only M. anisopliae significantly decreased D. suzukii survival, but it had low residual activity and no effect on D. suzukii fecundity. O. insidiosus decreased D. suzukii survival in simple laboratory arenas but not on potted blueberries or bagged blueberry branches outdoors. D. coriaria did not decrease D. suzukii survival in infested blueberries in simple laboratory arenas. The nematodes tested showed low infection rates and were not able to affect D. suzukii survival. Although this suite of natural enemies showed limited ability to suppress D. suzukii under the tested conditions, these and related natural enemies are present as part of the endemic natural enemy community in agricultural fields, where they may contribute to D. suzukii suppression. C1 [Woltz, J. M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Woltz, J. M.; Donahue, K. M.; Bruck, D. J.; Lee, J. C.] USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. RP Woltz, JM (reprint author), Oregon State Univ, Dept Hort, 4017 ALS, Corvallis, OR 97331 USA. EM woltzj@onid.orst.edu FU Northwest Center for Small Fruit Research, USDA SCRI Grant [2010-51181-21167]; Northwest Center for Small Fruit Research, USDA CRIS [5358-22000-037-00D] FX We are grateful to thank Austin Cuenca, Hannah Curry, Christina Fieland, Joe Kleiber, Jimmy Klick, Amanda Lake and Jesse Mindolovich for assistance with assays, and Jon Umbel at Fall Creek Nursery for providing blueberry plants for our potted plant trials. Funding was provided by the Northwest Center for Small Fruit Research, USDA SCRI Grant 2010-51181-21167 and USDA CRIS 5358-22000-037-00D. NR 35 TC 10 Z9 11 U1 14 U2 61 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD DEC PY 2015 VL 139 IS 10 BP 759 EP 770 DI 10.1111/jen.12200 PG 12 WC Entomology SC Entomology GA DA9QN UT WOS:000368144700005 ER PT J AU John, KMM Luthria, D AF John, K. M. Maria Luthria, Devanand TI Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties SO JOURNAL OF FOOD SCIENCE LA English DT Article DE amino acid; dry beans; gas and ion chromatography mass spectrometry; metabolic profiles; sugars ID NORTHEAST RED BEANS; BIOACTIVE COMPOUNDS; ASPERGILLUS-SOJAE; EXTRACTION; FERMENTATION; FRACTIONS; RESPONSES; LIQUID; LEAVES; CELLS AB In this study, we compared the amino acid, organic acid and sugar profiles of 3 different varieties of dry beans (black bean [BB], dark red bean [DRB], and cranberry bean [CB]). The efficiency of the 2 commonly used extraction solvents (water and methanol: chloroform: water [2.5:1:1, v/v/v/]) for cultivar differentiation based on their metabolic profile was also investigated. The results showed that the BB contained the highest concentration of amino acids followed by DRB and CB samples. Phenylalanine, a precursor for the biosynthesis of phenolic secondary metabolites was detected at low concentration in CB samples and correlated with the reduced anthocyanins content in CB extract as documented in the published literature. Comparing the extractability of 2 extraction solvents, methanol: chloroform: water (2.5: 1: 1, v/v/v/) showed higher recoveries of amino acids from 3 beans, whereas, sugars were extracted in higher concentration with water. Analytically, gas chromatography detected sugars (9), amino acids (11), and organic acids (3) in a single run after derivatization of the extracts. In comparison, ion chromatography detected only sugars in a single run without any derivatization step with the tested procedure. Bean samples are better differentiated by the sugar content extracted with water as compared to the aqueous organic solvent extracts using partial least-square discriminant analysis. C1 [John, K. M. Maria; Luthria, Devanand] USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit Methods Dev Lab, Beltsville, MD 20705 USA. RP Luthria, D (reprint author), USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit Methods Dev Lab, Beltsville, MD 20705 USA. EM Dave.Luthria@ars.usda.gov NR 32 TC 0 Z9 0 U1 5 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD DEC PY 2015 VL 80 IS 12 BP C2662 EP C2669 DI 10.1111/1750-3841.13115 PG 8 WC Food Science & Technology SC Food Science & Technology GA DA9MI UT WOS:000368132800005 PM 26509787 ER PT J AU Caldwell, JM Perez-Diaz, IM Harris, K Hassan, HM Simunovic, J Sandeep, KP AF Caldwell, Jane M. Perez-Diaz, Ilenys M. Harris, Keith Hassan, Hosni M. Simunovic, Josip Sandeep, K. P. TI Mitochondrial DNA Fragmentation to Monitor Processing Parameters in High Acid, Plant-Derived Foods SO JOURNAL OF FOOD SCIENCE LA English DT Article DE high-acid foods; mitochondrial DNA; quantitative PCR; thermal processing ID ESCHERICHIA-COLI O157-H7; APPLE CIDER; HEAT-RESISTANCE; TOMATO JUICE; TOLERANCE; SURVIVAL; SPORES; PCR AB Mitochondrial DNA (mtDNA) fragmentation was assessed in acidified foods. Using quantitative polymerase chain reaction, Ct values measured from fresh, fermented, pasteurized, and stored cucumber mtDNA were determined to be significantly different (P > 0.05) based on processing and shelf-life. This indicated that the combination of lower temperature thermal processes (hot-fill at 75 degrees C for 15 min) and acidified conditions (pH = 3.8) was sufficient to cause mtDNA fragmentation. In studies modeling high acid juices, pasteurization (96 degrees C, 0 to 24 min) of tomato serum produced Ct values which had high correlation to time-temperature treatment. Primers producing longer amplicons (approximately 1 kb) targeting the same mitochondrial gene gave greater sensitivity in correlating time-temperature treatments to Ct values. Lab-scale pasteurization studies using Ct values derived from the longer amplicon differentiated between heat treatments of tomato serum (95 degrees C for < 2 min). MtDNA fragmentation was shown to be a potential new tool to characterize low temperature (< 100 degrees C) high acid processes (pH < 4.6), nonthermal processes such as vegetable fermentation and holding times of acidified, plant-derived products. C1 [Caldwell, Jane M.; Harris, Keith; Simunovic, Josip; Sandeep, K. P.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Perez-Diaz, Ilenys M.] USDA ARS, SAA, Food Sci Res Unit, Raleigh, NC 27695 USA. [Hassan, Hosni M.] N Carolina State Univ, Prestage Dept Poultry Sci, Raleigh, NC 27695 USA. RP Caldwell, JM (reprint author), N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. EM jane.caldwell@transagra.com FU USDA-NIFA grant [2012-67017-30179]; Center for Advanced Processing and Packaging Studies (CAPPS) [0968960]; National Science Foundation FX The authors would like to acknowledge Janet Hayes for lab management, ordering supplies, and troubleshooting equipment; Sandra Parker for secretarial, grant, and travel assistance; Bernard Eckhardt for computer support; Dr. Suzanne Johanningsmeier, Emily Thorpe, and Lisa Rosenberg for pickle samples; and Joy Smith for statistical analysis. This research was funded by USDA-NIFA grant (2012-67017-30179) and a grant funded by the Center for Advanced Processing and Packaging Studies (CAPPS; 0968960), which is a center funded by the National Science Foundation. The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service of the products named nor criticism of similar ones not mentioned. An international and United States patent application has been filed for this process. NR 22 TC 1 Z9 2 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD DEC PY 2015 VL 80 IS 12 BP M2892 EP M2898 DI 10.1111/1750-3841.13139 PG 7 WC Food Science & Technology SC Food Science & Technology GA DA9MI UT WOS:000368132800032 PM 26556214 ER PT J AU Huang, LH AF Huang, Lihan TI Growth of Staphylococcus aureus in Cooked Potato and Potato Salad-A One-Step Kinetic Analysis SO JOURNAL OF FOOD SCIENCE LA English DT Article DE cooked potato; modelling; potato salad; Staphylococcus aureus ID UNITED-STATES; TEMPERATURE; MAYONNAISE; MODELS AB Staphylococcus aureus is a Gram-positive spherically-shaped bacterium capable of producing heat-stable enterotoxins that cause acute gastrointestinal diseases. The growth of this pathogen in food is a major threat to public health worldwide. Potato salad is a frequent vehicle for infection and food poisoning caused by S. aureus. Therefore, the objective of this study was to investigate the growth kinetics of S. aureus in cooked potato and potato salad. Samples of potato cubes and potato salad inoculated with S. aureus were incubated at temperatures between 8 and 43 degrees C to observe its growth for developing growth models. No growth was observed at 8 degrees C. The experimental results showed that the growth curves did not exhibit lag phases, and can be described by a 3-parameter logistic model. A one-step kinetic analysis approach was used to simultaneously analyze all growth curves by direct construction of both the primary and secondary (Ratkowsky square root) models using nonlinear regression to minimize the global residual error. The estimated nominal minimum growth temperature of S. aureus was 6.12 degrees C in potato cubes and 8.80 degrees C in potato salad. The estimated maximum growth temperatures of S. aureus in potato cubes and potato salad were very close to each other (46.3 and 46.8 degrees C, respectively). On the average, the specific growth rates of S. aureus in potato cubes were approximately 70% higher than those in potato salad. This study suggests that cooked potato and potato salad should be stored below 6 degrees C or above 47 degrees C to prevent the growth of S. aureus. The mathematical models and kinetic parameters can be used to accurately evaluate the effect of temperature abuse on the growth of S. aureus and conduct risk assessments of S. aureus in cooked potato and potato salad. C1 [Huang, Lihan] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 11 TC 1 Z9 1 U1 7 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD DEC PY 2015 VL 80 IS 12 BP M2837 EP M2844 DI 10.1111/1750-3841.13110 PG 8 WC Food Science & Technology SC Food Science & Technology GA DA9MI UT WOS:000368132800025 PM 26539902 ER PT J AU Perez-Diaz, IM McFeeters, RF Moeller, L Johanningsmeier, SD Hayes, J Fornea, DS Rosenberg, L Gilbert, C Custis, N Beene, K Bass, D AF Perez-Diaz, I. M. McFeeters, R. F. Moeller, L. Johanningsmeier, S. D. Hayes, J. Fornea, D. S. Rosenberg, L. Gilbert, C. Custis, N. Beene, K. Bass, D. TI Commercial Scale Cucumber Fermentations Brined with Calcium Chloride Instead of Sodium Chloride SO JOURNAL OF FOOD SCIENCE LA English DT Article DE sustainable processing; cucumber fermentation; low salt; vegetable preservation; chloride waste reduction ID LACTIC-ACID BACTERIA; FERMENTED CUCUMBER; SPOILAGE; YEASTS; PRESERVATION; CRISPNESS; CULTURE; STORAGE; FRUIT AB Development of low salt cucumber fermentation processes present opportunities to reduce the amount of sodium chloride (NaCl) that reaches fresh water streams from industrial activities. The objective of this research was to translate cucumber fermentation brined with calcium chloride (CaCl2) instead of NaCl to commercial scale production. Although CaCl2 brined cucumber fermentations were stable in laboratory experiments, commercial scale trials using 6440 L open-top tanks rapidly underwent secondary cucumber fermentation. It was understood that a limited air purging routine, use of a starter culture and addition of preservatives to the cover brine aids in achieving the desired complete cucumber fermentation. The modified process was used for subsequent commercial trials using 12490 and 28400 L opentop tanks packed with variable size cucumbers and from multiple lots, and cover brines containing CaCl2 and potassium sorbate to equilibrated concentrations of 100 and 6 mM, respectively. Lactobacillus plantarum LA0045 was inoculated to 106 CFU/mL, and air purging was applied for two 2-3 h periods per day for the first 10 d of fermentation and one 2-3 h period per day between days 11 and 14. All fermentations were completed, as evidenced by the full conversion of sugars to lactic acid, decrease in pH to 3.0, and presented microbiological stability for a minimum of 21 d. This CaCl2 process may be used to produce fermented cucumbers intended to be stored short term in a manner that reduces pollution and waste removal costs. C1 [Perez-Diaz, I. M.; McFeeters, R. F.; Johanningsmeier, S. D.; Hayes, J.; Fornea, D. S.] N Carolina State Univ, USDA, ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. [Moeller, L.; Beene, K.; Bass, D.] Mt Olive Pickle Co Inc, Mt Olive, NC 28365 USA. [Rosenberg, L.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Gilbert, C.; Custis, N.] B&G Foods Inc, Hurlock, MD 21643 USA. RP Perez-Diaz, IM (reprint author), N Carolina State Univ, USDA, ARS, SAA Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM ilenys.perez-diaz@ars.usda.gov FU B & G Foods, Inc., Sharptown, Md., U.S.A.; Mount Olive Pickle Co., Mount Olive, N.C., U.S.A; Gedney Foods, Chaska Minn., U.S.A. FX The authors would like to thank Mrs. Sandra Parker with the USDA-Agriculture Research Service, Food Science Research Unit for excellent administrative assistance. We also thank B & G Foods, Inc., Sharptown, Md., U.S.A.; Mount Olive Pickle Co., Mount Olive, N.C., U.S.A; and Gedney Foods, Chaska Minn., U.S.A. who supported this research in various ways. NR 26 TC 1 Z9 1 U1 7 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD DEC PY 2015 VL 80 IS 12 BP M2827 EP M2836 DI 10.1111/1750-3841.13107 PG 10 WC Food Science & Technology SC Food Science & Technology GA DA9MI UT WOS:000368132800024 PM 26512798 ER PT J AU Sman, AD Hackett, D Singh, MF Fornusek, C Menezes, MP Burns, J AF Sman, Amy D. Hackett, Daniel Singh, Maria Fiatarone Fornusek, Che Menezes, Manoj P. Burns, Joshua TI Systematic review of exercise for Charcot-Marie-Tooth disease SO JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM LA English DT Review DE Charcot-Marie-Tooth disease; exercise; hereditary motor and sensory neuropathy; strength; systematic review ID OVERWORK WEAKNESS; NEUROMUSCULAR DISEASE; ADULTS; NEUROPATHY; MUSCULOSKELETAL; INTERVENTIONS; HYPOTHESIS; CREATINE; QUALITY; FATIGUE AB Charcot-Marie-Tooth disease (CMT) is a slowly progressive hereditary degenerative disease and one of the most common neuromuscular disorders. Exercise may be beneficial to maintain strength and function for people with CMT, however, no comprehensive evaluation of the benefits and risks of exercise have been conducted. A systematic review was completed searching numerous electronic databases from earliest records to February 2015. Studies of any design including participants of any age with confirmed diagnosis of CMT that investigated the effects of exercise were eligible for inclusion. Of 13,301 articles identified following removal of duplicates, 11 articles including 9 unique studies met the criteria. Methodological quality of studies was moderate, sample sizes were small, and interventions and outcome measures used varied widely. Although the majority of the studies identified changes in one or more outcome measurements across exercise modalities, themajoritywere non-significant, possibly due to Type II errors. Significant effects described included improvements in strength, functional activities, and physiological adaptations following exercise. Despite many studies showing changes in strength and function following exercise, findings of this review should be met with caution due to the few studies available and moderate quality of evidence. Well-powered studies, harmonisation of outcome measures, and clearly described interventions across studies would improve the quality and comparability of the evidence base. The optimal exercise modality and intensity for people with CMT as well as the long-term safety of exercise remain unclear. C1 [Sman, Amy D.; Menezes, Manoj P.; Burns, Joshua] Childrens Hosp Westmead, Inst Neurosci & Muscle Res, Westmead, NSW 2145, Australia. [Sman, Amy D.; Burns, Joshua] Univ Sydney, Fac Hlth Sci, Discipline Physiotherapy, Lidcombe, Australia. [Sman, Amy D.; Burns, Joshua] Sydney Childrens Hosp Network Randwick & Westmead, Paediat Gait Anal Serv New South Wales, Sydney, NSW, Australia. [Hackett, Daniel; Singh, Maria Fiatarone; Fornusek, Che] Univ Sydney, Fac Hlth Sci, Discipline Exercise & Sport Sci, Lidcombe, Australia. [Singh, Maria Fiatarone] Univ Sydney, Sydney Med Sch, Camperdown, NSW, Australia. [Singh, Maria Fiatarone] Univ Sydney, Charles Perkins Ctr, Camperdown, NSW, Australia. [Singh, Maria Fiatarone] Hebrew Senior Life, Boston, MA 02111 USA. [Singh, Maria Fiatarone] Tufts Univ, Jean Meyer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Menezes, Manoj P.] Childrens Hosp Westmead, TY Nelson Dept Neurol & Neurosurg, Westmead, NSW, Australia. RP Sman, AD (reprint author), Childrens Hosp Westmead, Inst Neurosci & Muscle Res, Locked Bag 4001, Westmead, NSW 2145, Australia. EM amy.sman@sydney.edu.au OI Hackett, Daniel/0000-0002-2504-3942 NR 34 TC 4 Z9 4 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1085-9489 EI 1529-8027 J9 J PERIPHER NERV SYST JI J. Peripher. Nerv. Syst. PD DEC PY 2015 VL 20 IS 4 BP 347 EP 362 DI 10.1111/jns.12116 PG 16 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA DA9NK UT WOS:000368136000001 PM 26010435 ER PT J AU Cosentino, BJ Schooley, RL Bestelmeyer, BT McCarthy, AJ Sierzega, K AF Cosentino, Bradley J. Schooley, Robert L. Bestelmeyer, Brandon T. McCarthy, Alison J. Sierzega, Kevin TI Rapid genetic restoration of a keystone species exhibiting delayed demographic response SO MOLECULAR ECOLOGY LA English DT Article DE conservation genetics; density dependence; Dipodomys spectabilis; dispersal; ecological restoration; founder effects ID TAILED KANGAROO RATS; PLEISTOCENE CLIMATE CHANGES; MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; DIPODOMYS-SPECTABILIS; CHIHUAHUAN DESERT; CONNECTIVITY MEASURES; PRECAPTURE DISPERSAL; DENSITY-FLUCTUATIONS; COMMUNITY STRUCTURE AB Genetic founder effects are often expected when animals colonize restored habitat in fragmented landscapes, but empirical data on genetic responses to restoration are limited. We examined the genetic response of banner-tailed kangaroo rats (Dipodomys spectabilis) to landscape-scale grassland restoration in the Chihuahuan Desert of New Mexico, USA. Dipodomys spectabilis is a grassland specialist and keystone species. At sites treated with herbicide to remove shrubs, colonization by D. spectabilis is slow and populations persist at low density for >= 10 years (>= 6 generations). Persistence at low density and low gene flow may cause strong founder effects. We compared genetic structure of D. spectabilis populations between treated sites and remnant grasslands, and we examined how the genetic response to restoration depended on treatment age, area, and connectivity to source populations. Allelic richness and heterozygosity were similar between treated sites and remnant grasslands. Allelic richness at treated sites was greatest early in the restoration trajectory, and genetic divergence did not differ between recently colonized and established populations. These results indicated that founder effects during colonization of treated sites were weak or absent. Moreover, our results suggested founder effects were not mitigated by treatment area or connectivity. Dispersal is negatively density-dependent in D. spectabilis, and we hypothesize that high gene flow may occur early in the restoration trajectory when density is low. Our study shows genetic diversity can be recovered more rapidly than demographic components of populations after habitat restoration and that founder effects are not inevitable for animals colonizing restored habitat in fragmented landscapes. C1 [Cosentino, Bradley J.; McCarthy, Alison J.] Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. [Schooley, Robert L.; Sierzega, Kevin] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. RP Cosentino, BJ (reprint author), Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. EM cosentino@hws.edu FU USDA-AFRI Managed Ecosystems program; BLM; Hobart and William Smith Colleges FX This research was funded by the USDA-AFRI Managed Ecosystems program, the BLM, and Hobart and William Smith Colleges. We thank P. Waser, A. DeWoody and J. Busch for providing helpful guidance on PCR conditions, for the microsatellites, and B. Dehond and J. Kurbs, for assistance in the laboratory. L. Burkett and R. Lister were instrumental for providing logistical support in the field. Animal care and handling was covered by a collection permit from the New Mexico Department of Game and Fish (3501) and a University of Illinois Institutional Animal Care and Use Committee protocol (09259). NR 94 TC 0 Z9 0 U1 7 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD DEC PY 2015 VL 24 IS 24 BP 6120 EP 6133 DI 10.1111/mec.13469 PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DA6IM UT WOS:000367906700009 PM 26577599 ER PT J AU Meyer, SE Masi, M Clement, S Davis, TL Beckstead, J AF Meyer, S. E. Masi, M. Clement, S. Davis, T. L. Beckstead, J. TI Mycelial growth rate and toxin production in the seed pathogen Pyrenophora semeniperda: resource trade-offs and temporally varying selection SO PLANT PATHOLOGY LA English DT Article DE Bromus tectorum; co-inoculation; competition; cytochalasin B; seed bank; seed dormancy ID BROMUS-TECTORUM; PLANT PATHOGEN; POTENTIAL MYCOHERBICIDE; LATENT PERIOD; VIRULENCE; EVOLUTION; CONSEQUENCES; AGGRESSIVENESS; COEVOLUTION; POPULATION AB Pyrenophora semeniperda, an important pathogen in Bromus tectorum seed banks in semi-arid western North America, exhibits >4-fold variation in mycelial growth rate. Host seeds exhibit seasonal changes in dormancy that affect the risk of pathogen-caused mortality. The hypothesis tested is that contrasting seed dormancy phenotypes select for contrasting strategies for increasing pathogen fitness, and that increased fitness on nondormant seeds involves a resource trade-off between toxin production and growth. The strategy for successfully attacking rapidly germinating nondormant seeds at high inoculum loads in autumn involves increased post-infection aggressiveness to prevent seed escape through germination. An earlier study demonstrated that slow-growing strains caused higher mortality than faster-growing strains on nondormant host seeds at high inoculum loads. In this study, production of the toxin cytochalasin B was significantly higher in slower-growing strains, and was induced only in seeds or in seed-constituent-containing media. Its production was reduced in vivo by Bromus tectorum seeds, suggesting direct involvement in pathogenesis on seeds. Fast-growing strains caused significantly higher mortality than slow-growing strains at low inoculum loads on dormant seeds, which apparently have resistance that is overcome at high loads or through rapid mycelial proliferation. In a co-inoculation study, the fast-growing isolate produced 3 9 more stromata than the slow-growing isolate on dormant seeds, whereas the slow-growing isolate was twice as successful on nondormant seeds. These results provide evidence that mycelial growth rate variation and associated variation in cytochalasin B production represent a trade-off maintained through temporally varying selection resulting from seasonal variation in host seed dormancy status. C1 [Meyer, S. E.; Clement, S.] US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, Provo, UT 84606 USA. [Masi, M.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84606 USA. [Davis, T. L.; Beckstead, J.] Gonzaga Univ, Dept Biol, Spokane, WA 99258 USA. RP Meyer, SE (reprint author), US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, Provo, UT 84606 USA. EM smeyer@fs.fed.us RI Masi, Marco/J-7742-2016 OI Masi, Marco/0000-0003-0609-8902 FU US Department of Agriculture, Cooperative State Research, Extension, and Education Service, National Research Initiative, Biology of Weedy and Invasive Species Program [2008-35320-18677]; Joint Fire Sciences Program of the US Departments of Agriculture and Interior [JFSP-11-S-2-6]; US Forest Service State and Private Forestry BCIP (Biological Control of Invasive Plants) Program; Weed Science Society of America Undergraduate Research Grant FX Thanks to Joshua Nicholson and Megan Taylor for help with the ring microcosm study, to Steven Harrison, Erin Heckaman, and Kristina Bair for assistance with microsatellite-genotyping samples for the co-inoculation study, and to Beth Black for help with sample preparation for the HPLC analyses. Logistical support at Brigham Young University was provided to M. Masi by Dr P. Savage and Dr J. Price of the Chemistry Department. The authors also gratefully acknowledge M. Smith and P. Laurence, who assisted with the HPLC analyses carried out at Brigham Young University. This research was funded in part by grant no. 2008-35320-18677 to S.E.M. and J.B. from the US Department of Agriculture, Cooperative State Research, Extension, and Education Service, National Research Initiative, Biology of Weedy and Invasive Species Program, by grant no. JFSP-11-S-2-6 to S.E.M. and J.B. from the Joint Fire Sciences Program of the US Departments of Agriculture and Interior, and by a grant to S.E.M. from the US Forest Service State and Private Forestry BCIP (Biological Control of Invasive Plants) Program. T.L.D. received support from a Weed Science Society of America Undergraduate Research Grant in 2009. NR 35 TC 1 Z9 1 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD DEC PY 2015 VL 64 IS 6 BP 1450 EP 1460 DI 10.1111/ppa.12377 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DA7AH UT WOS:000367956500019 ER PT J AU Gray, MJ Lewis, JP Nanjappa, P Klocke, B Pasmans, F Martel, A Stephen, C Olea, GP Smith, SA Sacerdote-Velat, A Christman, MR Williams, JM Olson, DH AF Gray, Matthew J. Lewis, James P. Nanjappa, Priya Klocke, Blake Pasmans, Frank Martel, An Stephen, Craig Parra Olea, Gabriela Smith, Scott A. Sacerdote-Velat, Allison Christman, Michelle R. Williams, Jennifer M. Olson, Deanna H. TI Batrachochytrium salamandrivorans: The North American Response and a Call for Action SO PLOS PATHOGENS LA English DT Article ID EMERGING DISEASE; AMPHIBIANS; CHYTRIDIOMYCOSIS; SALAMANDERS; FORESTS C1 [Gray, Matthew J.] Univ Tennessee, Ctr Wildlife Hlth, Knoxville, TN USA. [Lewis, James P.; Klocke, Blake] Amphibian Survival Alliance, Austin, TX USA. [Nanjappa, Priya] Assoc Fish & Wildlife Agencies, Washington, DC USA. [Klocke, Blake] George Mason Univ, Dept Environm Sci & Policy, Fairfax, VA 22030 USA. [Pasmans, Frank; Martel, An] Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Avian Dis, Merelbeke, Belgium. [Stephen, Craig] Canadian Wildlife Hlth Cooperat, Saskatoon, SK, Canada. [Parra Olea, Gabriela] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico. [Smith, Scott A.] Maryland Dept Nat Resources, Wye Mills, MD USA. [Sacerdote-Velat, Allison] Lincoln Pk Zoo, Chicago, IL USA. [Christman, Michelle R.] US Fish & Wildlife Serv, New Mexico Ecol Serv Field Off, Albuquerque, NM USA. [Williams, Jennifer M.] Partners Amphibian & Reptile Conservat, Ft Collins, CO USA. [Olson, Deanna H.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Gray, MJ (reprint author), Univ Tennessee, Ctr Wildlife Hlth, Knoxville, TN USA. EM mgray11@utk.edu RI Pasmans, Frank/D-3667-2014 NR 26 TC 5 Z9 5 U1 16 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD DEC PY 2015 VL 11 IS 12 AR e1005251 DI 10.1371/journal.ppat.1005251 PG 9 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA DB2IZ UT WOS:000368332800006 PM 26662103 ER PT J AU Diebel, MW Fedora, M Cogswell, S O'Hanley, JR AF Diebel, M. W. Fedora, M. Cogswell, S. O'Hanley, J. R. TI EFFECTS OF ROAD CROSSINGS ON HABITAT CONNECTIVITY FOR STREAM-RESIDENT FISH SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE road crossings; fish passage; stream connectivity; barrier removal; prioritization ID PASSAGE BARRIERS; MOVEMENT PATTERNS; TROUT; FRAGMENTATION; REMOVAL; RESTORATION; MANAGEMENT; CULVERTS; ASSEMBLAGES; IMPACT AB Road crossings can act as barriers to the movement of stream fishes, resulting in habitat fragmentation, reduced population resilience to environmental disturbance and higher risks of extinction. Strategic barrier removal has the potential to improve connectivity in stream networks, but managers lack a consistent framework for determining which projects will most benefit target species. The objective of this study is to develop a method for identifying and prioritizing action on road crossings in order to restore stream network connectivity. We demonstrate the method using a case study from the Pine-Popple watershed in Wisconsin. First, we propose a new metric for quantifying stream connectivity status for stream-resident fish. The metric quantifies the individual and cumulative effects of barriers on reach and watershed level connectivity, while accounting for natural barriers, distance-based dispersal limitations and variation in habitat type and quality. We conducted a comprehensive field survey of road crossings in the watershed to identify barriers and estimate replacement costs. Of the 190 surveyed road crossings, 74% were determined to be barriers to the movement of at least one species or life stage of fish, primarily due to high water velocity, low water depth or outlet drops. The results of the barrier removal prioritization show that initial projects targeted for mitigation create much greater improvements in connectivity per unit cost than later projects. Benefit-cost curves from this type of analysis can be used to evaluate potential projects within and among watersheds and minimize overall expenditures for specified restoration targets. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Diebel, M. W.] Wisconsin Dept Nat Resources, Madison, WI 53711 USA. [Fedora, M.] US Forest Serv, USDA, Ironwood, MI USA. [Cogswell, S.] US Fish & Wildlife Serv, Green Bay, WI USA. [O'Hanley, J. R.] Univ Kent, Kent Business Sch, Canterbury, Kent, England. RP Diebel, MW (reprint author), Wisconsin Dept Nat Resources, 2801 Progress Rd, Madison, WI 53711 USA. EM matthew.diebel@wisconsin.gov FU The Nature Conservancy; US Fish & Wildlife Service FX This research was funded by grants from The Nature Conservancy and the US Fish & Wildlife Service, which helped to support M. W. D. The authors appreciate assistance by J. Maxted with the GIS analysis and J. Simonsen, D. Higgins, T. Pearson, M. Steiger and Y. Malca with field surveys. NR 41 TC 4 Z9 4 U1 11 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD DEC PY 2015 VL 31 IS 10 BP 1251 EP 1261 DI 10.1002/rra.2822 PG 11 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DA7UR UT WOS:000368010500005 ER PT J AU Wheeler, CA Bettaso, JB Ashton, DT Welsh, HH AF Wheeler, C. A. Bettaso, J. B. Ashton, D. T. Welsh, H. H., Jr. TI EFFECTS OF WATER TEMPERATURE ON BREEDING PHENOLOGY, GROWTH, AND METAMORPHOSIS OF FOOTHILL YELLOW-LEGGED FROGS (RANA BOYLII): A CASE STUDY OF THE REGULATED MAINSTEM AND UNREGULATED TRIBUTARIES OF CALIFORNIA'S TRINITY RIVER SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE Anuran development; body condition; water thermal regime; stream anuran ID ANURAN METAMORPHOSIS; FOOD LEVEL; GREEN FROG; LARVAL; POPULATION; DENSITY; SYLVATICA; HISTORY; SIZE; RECRUITMENT AB Many riverine organisms are well adapted to seasonally dynamic environments, but extreme changes in flow and thermal regimes can threaten sustainability of their populations in regulated rivers. Altered thermal regimes may limit recruitment to populations by shifting the timing of breeding activities and affecting the growth and development of early life stages. Stream-dwelling anurans such as the foothill yellow-legged frog (Rana boylii) in the Trinity River of northern California are model subjects for examining associations between water temperature and the timing of oviposition, hatching, and metamorphosis, and body condition and size of tadpoles and metamorphs. Breeding activity, hatching success, and metamorphosis occurred later, and metamorphs were smaller and leaner along the regulated and colder mainstem relative to six unregulated tributaries of the Trinity River. Persistently depressed summer water temperatures appear to play a seminal role in inhibited tadpole growth on the regulated mainstem and may be a causative factor in the pronounced decline of this population. Environmental flow assessments should account for the influence of the thermal regime on the development of vulnerable embryonic and larval life stages to improve outcomes for declining amphibian populations. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Wheeler, C. A.; Ashton, D. T.; Welsh, H. H., Jr.] USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. [Bettaso, J. B.] US Fish & Wildlife Serv, E Lansing, MI USA. RP Wheeler, CA (reprint author), USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM cawheeler@fs.fed.us NR 46 TC 5 Z9 5 U1 10 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD DEC PY 2015 VL 31 IS 10 BP 1276 EP 1286 DI 10.1002/rra.2820 PG 11 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DA7UR UT WOS:000368010500007 ER PT J AU Bassett, CL Fisher, KM Farrell, RE AF Bassett, Carole Leavel Fisher, Kirsten M. Farrell, Robert E., Jr. TI The complete peach dehydrin family: characterization of three recently recognized genes SO TREE GENETICS & GENOMES LA English DT Article DE Abiotic stress; Gene expression; Promoter comparison; Woody plants ID ACTING REGULATORY ELEMENTS; LOW-TEMPERATURE; ARABIDOPSIS-THALIANA; PRUNUS-PERSICA; SUBCELLULAR-LOCALIZATION; PREDICTION SERVER; WATER-DEFICIT; ION-BINDING; DNA-BINDING; IN-VIVO AB Three genes encoding dehydrins have been previously described from peach. In the course of searching the peach genome, three additional members of this stress-associated family were recognized, PpDhn4-6. PpDhn1 and 6 have no introns, whereas the remaining four genes have a single intron located near the 3 ' end of the serine (S) tract. PpDHN2 was the only dehydrin with a predicted basic pI; pI predictions for the other dehydrins ranged from about 5.3 to about 6.3. None of the peach dehydrins have tryptophan residues, but, in contrast to most dehydrins, three (PpDHN1, 3, and 4) have one or more cysteine residues. Phylogenetic analysis indicated a close relationship between PpDhn1 and 6 and PpDhn3 and 4. Expression analysis under low temperature and dehydration confirmed that PpDhn2 is the major responder to drought, while both PpDhn1 and 6 respond exclusively to cold. Comparison of the first 500 base pairs upstream of the translation start site revealed the presence of cis-elements associated with low temperature and drought/osmotic/salt and hormone response regulation. C1 [Bassett, Carole Leavel] ARS, USDA, Appalachian Fruit Res Stn, Keedysville, MD 21756 USA. [Fisher, Kirsten M.] Calif State Univ Los Angeles, Dept Biol Sci, Los Angeles, CA 90032 USA. [Farrell, Robert E., Jr.] Penn State Univ, Dept Biol, York, PA 17403 USA. RP Bassett, CL (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 18933 Planters Lane, Keedysville, MD 21756 USA. EM clbnhb@yahoo.com; kfisher2@calstatela.edu; jrf10@psu.edu FU USDA, the Agricultural Research Service, CRIS project [8080-21000-022-00D] FX We appreciate the expert technical assistance of Ms. Jing Ma in the expression studies. This work was supported in its entirety by the USDA, the Agricultural Research Service, CRIS project 8080-21000-022-00D. NR 74 TC 1 Z9 1 U1 4 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD DEC PY 2015 VL 11 IS 6 AR 126 DI 10.1007/s11295-015-0923-5 PG 14 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA DA6FM UT WOS:000367898000002 ER PT J AU He, XH Quinones, B Te Loo, M Loos, S Scavia, G Brigotti, M Levtchenko, E Monnens, L AF He, Xiaohua Quinones, Beatriz Te Loo, Maroeska Loos, Sebastian Scavia, Gaia Brigotti, Maurizio Levtchenko, Elena Monnens, Leo TI Serum Shiga toxin 2 values in patients during acute phase of diarrhoea-associated haemolytic uraemic syndrome SO ACTA PAEDIATRICA LA English DT Article DE Foodborne pathogen; Functional assay of Stx-2; Haemolytic uraemic syndrome; Shiga toxin ID AMYLOID-P COMPONENT; ESCHERICHIA-COLI; MONOCLONAL-ANTIBODIES; ENDOTHELIAL-CELLS; IN-VIVO; BLOOD; MODEL AB Aim: Shiga toxins are delivered via systemic circulation and are considered to be the cause of diarrhoea-associated haemolytic uraemic syndrome (HUS), as they injure endothelial cells, particularly in the glomeruli. This study measured Shiga toxin 2 (Stx2) in the serum of children affected in by HUS due to Stx2 producing Escherichia coli. Methods: The concentration of free Stx2 was measured in the serum of 16 children, collected immediately after admission to the clinic in the acute phase of HUS, using a sandwich enzyme-linked immunosorbent assay. The family members of two children were also investigated, with the relative toxicity of Stx2 assessed by a Vero cell-based fluorescent assay. Results: Stx2 was found in the serum of eight of the 16 children who were investigated. It was also detected in four of the six family members not showing symptomatic HUS, with an extremely high level in two. Conclusion: An absent or rather low concentration of Stx2 was found in the serum of children admitted to the clinic with diarrhoea-associated HUS. The high concentration of Stx2 in family members without HUS, but mostly with watery diarrhoea and raised functional activity, was in line with the concept of early injury by Stx2. C1 [He, Xiaohua] ARS, USDA, WRRC, Foodborne Toxin Detect & Prevent Unit, Albany, NY USA. [Quinones, Beatriz] ARS, USDA, WRRC, Produce Safety & Microbiol Unit, Albany, NY USA. [Te Loo, Maroeska] Radboud Univ Nijmegen, Med Ctr, Dept Pediat, NL-6500H Nijmegen, Netherlands. [Loos, Sebastian] Univ Med Ctr, Div Pediat Nephrol, Dept Pediat, Hamburg, Germany. [Scavia, Gaia] Inst Super Sanita, EU Reference Lab E Coli, Rome, Italy. [Brigotti, Maurizio] Univ Bologna, Dipartimento Med Specialist Diagnost & Sperimenta, Sede Patol Gen, Bologna, Italy. [Levtchenko, Elena] Univ Hosp Leuven, Dept Pediat Nephrol & Growth & Regenerat, Leuven, Belgium. [Monnens, Leo] Radboud Univ Nijmegen, Nijmegen Med Ctr, Dept Physiol, NL-6500H Nijmegen, Netherlands. RP Monnens, L (reprint author), Radboud Univ Nijmegen, Nijmegen Med Ctr, Dept Physiol, POB 9101, NL-6500H Nijmegen, Netherlands. EM leo.monnens@radboudumc.nl NR 22 TC 1 Z9 1 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0803-5253 EI 1651-2227 J9 ACTA PAEDIATR JI Acta Paediatr. PD DEC PY 2015 VL 104 IS 12 BP E564 EP E568 DI 10.1111/apa.13211 PG 5 WC Pediatrics SC Pediatrics GA DA1DH UT WOS:000367536000009 PM 26361237 ER PT J AU Ro, KS Lima, IM Reddy, GB Jackson, MA Gao, B AF Ro, Kyoung S. Lima, Isabel M. Reddy, Guidqopuram B. Jackson, Michael A. Gao, Bin TI Removing Gaseous NH3 Using Biochar as an Adsorbent SO AGRICULTURE-BASEL LA English DT Article DE gaseous ammonia; biochar; adsorption; activation; regeneration ID ACTIVATED-CARBON; AMMONIA ADSORPTION; TEMPERATURE; PYROLYSIS; CHARCOAL; MANURE AB Ammonia is a major fugitive gas emitted from livestock operations and fertilization production. This study tested the potential of various biochars in removing gaseous ammonia via adsorption processes. Gaseous ammonia adsorption capacities of various biochars made from wood shaving and chicken litter with different thermal conditions and activation techniques were determined using laboratory adsorption column tests. Ammonia adsorption capacities of non-activated biochars ranged from 0.15 to 5.09mgN/g, which were comparable to that of other commercial activated carbon and natural zeolite. There were no significant differences in ammonia adsorption capacities of steam activated and non-activated biochars even if the surface areas of the steam activated biochars were about two orders of magnitude greater than that of non-activated biochars. In contrast, phosphoric acid activation greatly increased the biochar ammonia adsorption capacity. This suggests that the surface area of biochar did not readily control gaseous NH3 adsorption. Ammonia adsorption capacities were more or less linearly increased with acidic oxygen surface groups of non-activated and steam-activated biochars. Phosphoric acid bound to the acid activated biochars is suspected to contribute to the exceptionally high ammonia adsorption capacity. The sorption capacities of virgin and water-washed biochar samples were not different, suggesting the potential to regenerate spent biochar simply with water instead of energy- and capital-intensive steam. The results of this study suggest that non-activated biochars can successfully replace commercial activated carbon in removing gaseous ammonia and the removal efficiency will greatly increase if the biochars are activated with phosphoric acid. C1 [Ro, Kyoung S.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Lima, Isabel M.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Reddy, Guidqopuram B.] North Carolina Agr & Tech State Univ, Dept Nat Resources, Greensboro, NC 27411 USA. [Jackson, Michael A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Gao, Bin] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. RP Ro, KS (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. EM kyoung.ro@ars.usda.gov; isabel.lima@ars.usda.gov; reddyg@ncat.edu; michael.jackson@ars.usda.gov; bg55@ufl.edu RI Gao, Bin/B-8027-2010 OI Gao, Bin/0000-0003-3769-0191 NR 25 TC 3 Z9 3 U1 6 U2 21 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD DEC PY 2015 VL 5 IS 4 BP 991 EP 1002 DI 10.3390/agriculture5040991 PG 12 WC Agronomy SC Agriculture GA DA2CM UT WOS:000367602900004 ER PT J AU Ducey, TF Novak, JM Johnson, MG AF Ducey, Thomas F. Novak, Jeffrey M. Johnson, Mark G. TI Effects of Biochar Blends on Microbial Community Composition in Two Coastal Plain Soils SO AGRICULTURE-BASEL LA English DT Article DE biochar; soil microbiology; southeastern United States ID FATTY-ACID PROFILES; PHOSPHORUS LIMITATION; SWITCHGRASS BIOCHAR; TEMPERATE SOILS; PLANT-GROWTH; WHEAT CROP; NITROGEN; MANAGEMENT; BIOMASS; FIELD AB The amendment of soil with biochar has been demonstrated to have an effect not only on the soil physicochemical properties, but also on soil microbial community composition and activity. Previous reports have demonstrated significant impacts on soil microbial community structure. These impacts are modulated not only by the biochar composition, but also on the soil's physicochemical characteristics. This indicates that soil characteristics must be considered prior to biochar amendment. A significant portion of the soils of the southeastern coastal plain are severely degraded and, therefore, candidates for biochar amendment to strengthen soil fertility. In this study we focused on two common soil series in the southeastern coastal plain, utilizing feedstocks endemic to the area. We chose feedstocks in four ratios (100% pine chip; 80:20 mixture of pine chip to poultry litter; 50:50 mixture of pine chip to poultry litter; 100% poultry litter) prior to pyrolysis and soil amendment as a biochar product. Soil was analyzed for bioavailable nutrients via Mehlich-1 extractions, as well as microbial community composition using phospholipid fatty acid analysis (PLFA). Our results demonstrated significant shifts in microbial community composition in response to biochar amendment, the effects of which were greatest with 100% poultry litter biochar. Strong relationships between PLFAs and several Mehlich-1 extractable nutrients (Al, Cu, Fe, and P) were observed. C1 [Ducey, Thomas F.; Novak, Jeffrey M.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Johnson, Mark G.] US EPA, Western Ecol Div, Natl Hlth & Environm Effects Res Lab, Corvallis, OR 97333 USA. RP Ducey, TF (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC 29501 USA. EM thomas.ducey@ars.usda.gov; jeff.novak@ars.usda.gov; johnson.mark@epa.gov NR 40 TC 2 Z9 2 U1 7 U2 8 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD DEC PY 2015 VL 5 IS 4 BP 1060 EP 1075 DI 10.3390/agriculture5041060 PG 16 WC Agronomy SC Agriculture GA DA2CM UT WOS:000367602900009 ER PT J AU Soti, P Fleurissaint, A Reed, S Jayachandran, K AF Soti, Pushpa Fleurissaint, Angie Reed, Stewart Jayachandran, Krish TI Effects of Control Release Fertilizers on Nutrient Leaching, Palm Growth and Production Cost SO AGRICULTURE-BASEL LA English DT Article DE slow release fertilizers; palm growth; nutrient leaching; horticulture ID PHOSPHORUS; NITROGEN; PATTERNS AB Objective of this study was to evaluate the effect of different controlled release fertilizer technologies on nutrient leaching and plant growth parameters of two palm species, Chinese Fan (Livistona chinensis) and Queen (Syagrus romanzoffiana). We compared Nutri-Pak (12-4-12 controlled release packet) and Harrell's (12-4-12 controlled release polymer coated urea) against Atlantic (8-4-12 controlled release polymer coated urea, coated sulfate of potash), the most commonly used palm fertilizer in South Florida. Plants were grown in 25 cm (11 L) pots under 70% shade, watered weekly, with pest and weed control done as required. Plant growth parameters: number of leaves, leaf length and width, and basal diameter, were measured every two months. Leachate was collected weekly after irrigation and a two-month composite sample was analyzed for nutrient concentrations. There was no difference in the growth parameters among the three fertilizers for Chinese Fan plants. However for Queen, Atlantic and Harrell's had significantly thicker basal diameter than Nutri-Pak. Significant difference in the concentration of nutrients in the leachate was observed among the fertilizer types. Throughout the study period, Nutri-Pak had a lower concentration of nutrients in the leachate than Atlantic and Harrell's. Our research indicates that Nutri-Pak control release fertilizer is comparable to other commercial fertilizers in Chinese Fan growth, but the larger Queen palms likely require an additional packet. Nutri-Pak fertilizer resulted in less nutrient leaching and could be a better environmental choice. C1 [Soti, Pushpa] Univ Texas Rio Grande Valley, Dept Biol, Edinburg, TX 78539 USA. [Fleurissaint, Angie; Jayachandran, Krish] Florida Int Univ, Dept Earth & Environm, Agroecol Program, Miami, FL 33199 USA. [Reed, Stewart] ARS, USDA, Miami, FL 33158 USA. RP Reed, S (reprint author), ARS, USDA, Miami, FL 33158 USA. EM psoti001@fiu.edu; afleu005@fiu.edu; stewart.reed@ars.usda.gov; jayachan@fiu.edu NR 16 TC 0 Z9 0 U1 2 U2 7 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD DEC PY 2015 VL 5 IS 4 BP 1135 EP 1145 DI 10.3390/agriculture5041135 PG 11 WC Agronomy SC Agriculture GA DA2CM UT WOS:000367602900012 ER PT J AU Boyette, CD Hoagland, RE Stetina, KC AF Boyette, Clyde D. Hoagland, Robert E. Stetina, Kenneth C. TI Biological Control of Spreading Dayflower (Commelina diffusa) with the Fungal Pathogen Phoma commelinicola SO AGRONOMY-BASEL LA English DT Article DE bioherbicide; biocontrol; dayflower; fungal pathogen; weed control ID WEED MANAGEMENT; FIELD BINDWEED; UNITED-STATES; CONTROL AGENT; PROBOSCIS; TEMPERATURE; GLYPHOSATE; HERBARUM; EFFICACY; DISEASE AB Greenhouse and field experiments showed that conidia of the fungal pathogen, Phoma commelinicola, exhibited bioherbicidal activity against spreading dayflower ( Commelina diffusa) seedlings when applied at concentrations of 106 to 109 conidia center dot mL-1. Greenhouse tests determined an optimal temperature for conidial germination of 25 degrees C- 30 degrees C, and that sporulation occurred on several solid growth media. A dew period of >= 12 h was required to achieve 60% control of cotyledonary-first leaf growth stage seedlings when applications of 108 conidia center dot mL-1 were applied. Maximal control ( 80%) required longer dew periods ( 21 h) and 90% plant dry weight reduction occurred at this dew period duration. More efficacious control occurred on younger plants ( cotyledonary- first leaf growth stage) than older, larger plants. Mortality and dry weight reduction values in field experiments were similar to 70% and > 80%, respectively, when cotyledonary- third leaf growth stage seedlings were sprayed with 108 or 109 conidia center dot mL-1. These results indicate that this fungus has potential as a biological control agent for controlling this problematic weed that is tolerant to the herbicide glyphosate. C1 [Boyette, Clyde D.; Stetina, Kenneth C.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Hoagland, Robert E.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Boyette, CD (reprint author), USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM doug.boyette@ars.usda.gov; bob.hoagland@ars.usda.gov; kenneth.stetina@ars.usda.gov NR 54 TC 0 Z9 0 U1 5 U2 5 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4395 J9 AGRONOMY-BASEL JI Agronomy-Basel PD DEC PY 2015 VL 5 IS 4 BP 519 EP 536 DI 10.3390/agronomy5040519 PG 18 WC Agronomy SC Agriculture GA DA2HE UT WOS:000367615100004 ER PT J AU Eisenman, D McCaffrey, S Donatello, I Marshal, G AF Eisenman, David McCaffrey, Sarah Donatello, Ian Marshal, Grant TI An Ecosystems and Vulnerable Populations Perspective on Solastalgia and Psychological Distress After a Wildfire SO ECOHEALTH LA English DT Article DE wildfire; mental health; solastalgia; disaster; resilience; climate change ID WILDLAND-URBAN INTERFACE; SERIOUS MENTAL-ILLNESS; GENERAL-POPULATION; SCREENING SCALES; CLIMATE-CHANGE; UNITED-STATES; HEALTH; DISASTER; CHILDREN; K10 AB We studied the relationship between psychological distress and relative resource and risk predictors, including loss of solace from the landscape (solastalgia), one year after the Wallow Fire, in Arizona, United States. Solastalgia refers to the distress caused by damage to the surrounding natural environment and it has not been examined for its relationship to psychological health. Doing so opens avenues of research that inquire into how land management might be able to support improved community resilience and psychological health outcomes after a wildfire. In 2012, we conducted a household survey mailed to all 1387 households in the five communities surrounding the fire. The Kessler Psychological Distress Scale assessed psychological distress. In the multivariate analysis, higher solastalgia score and an adverse financial impact of the fire were associated with clinically significant psychological distress. Annual household income a parts per thousand yen $80,000 and a higher family functioning score were associated with less psychological distress. Part-time residents were no more likely to have psychological distress than full-time residents. We conclude that dramatic transformation of a landscape by an environmental event such as a wildfire can reduce its value as a source of solace. These results call for novel post-wildfire community recovery interventions that wed forest management and community psychology. C1 [Eisenman, David] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Gen Internal Med & Hlth Serv Res, Los Angeles, CA 90024 USA. [Eisenman, David; Donatello, Ian] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Ctr Publ Hlth & Disasters, Los Angeles, CA 90095 USA. [McCaffrey, Sarah] US Forest Serv, USDA, No Res Stn, Evanston, IL 60201 USA. [Marshal, Grant] RAND Corp, Santa Monica, CA 90407 USA. RP Eisenman, D (reprint author), Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Gen Internal Med & Hlth Serv Res, 911 Broxton Plaza, Los Angeles, CA 90024 USA. EM deisenman@mednet.ucla.edu; smccaffrey@fs.fed.us; idonatello@gmail.com; grantm@rand.org FU U.S. Department of Agriculture/Forest Service [09-JV-11242309-042] FX Funding provided by the U.S. Department of Agriculture/Forest Service, Award #09-JV-11242309-042. We also thank Greg Winter, Cornerstone Strategies Inc. and Kellie Monterrosa, M.B.A., Apache County Department of Public Health. NR 40 TC 0 Z9 0 U1 3 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD DEC PY 2015 VL 12 IS 4 BP 602 EP 610 DI 10.1007/s10393-015-1052-1 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DA2LW UT WOS:000367627300009 PM 26302957 ER PT J AU Dudley, JP Hoberg, EP Jenkins, EJ Parkinson, AJ AF Dudley, Joseph P. Hoberg, Eric P. Jenkins, Emily J. Parkinson, Alan J. TI Climate Change in the North American Arctic: A One Health Perspective SO ECOHEALTH LA English DT Review DE climate change; cultural resilience; environmental pollutants; indigenous peoples; One Health; parasites; wildlife diseases; zoonosis ID PERSISTENT ORGANIC POLLUTANTS; INFLUENZA-A VIRUSES; WEST-NILE-VIRUS; BEARS URSUS-MARITIMUS; AVIAN INFLUENZA; POLAR BEARS; ENVIRONMENTAL CONTAMINANTS; POLYCHLORINATED-BIPHENYLS; TOXOPLASMA-GONDII; ECOSYSTEM HEALTH AB Climate change is expected to increase the prevalence of acute and chronic diseases among human and animal populations within the Arctic and subarctic latitudes of North America. Warmer temperatures are expected to increase disease risks from food-borne pathogens, water-borne diseases, and vector-borne zoonoses in human and animal populations of Arctic landscapes. Existing high levels of mercury and persistent organic pollutant chemicals circulating within terrestrial and aquatic ecosystems in Arctic latitudes are a major concern for the reproductive health of humans and other mammals, and climate warming will accelerate the mobilization and biological amplification of toxic environmental contaminants. The adverse health impacts of Arctic warming will be especially important for wildlife populations and indigenous peoples dependent upon subsistence food resources from wild plants and animals. Additional research is needed to identify and monitor changes in the prevalence of zoonotic pathogens in humans, domestic dogs, and wildlife species of critical subsistence, cultural, and economic importance to Arctic peoples. The long-term effects of climate warming in the Arctic cannot be adequately predicted or mitigated without a comprehensive understanding of the interactive and synergistic effects between environmental contaminants and pathogens in the health of wildlife and human communities in Arctic ecosystems. The complexity and magnitude of the documented impacts of climate change on Arctic ecosystems, and the intimacy of connections between their human and wildlife communities, makes this region an appropriate area for development of One Health approaches to identify and mitigate the effects of climate warming at the community, ecosystem, and landscape scales. C1 [Dudley, Joseph P.] Leidos Inc, Germantown, MD 20874 USA. [Dudley, Joseph P.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Hoberg, Eric P.] USDA ARS, US Natl Parasite Collect, Beltsville, MD 20705 USA. [Jenkins, Emily J.] Univ Saskatchewan, Dept Vet Microbiol, Saskatoon, SK S7N 5B4, Canada. [Parkinson, Alan J.] Ctr Dis Control & Prevent, Arctic Invest Program, Div Preparedness & Emerging Infect, Natl Ctr Emerging & Zoonot Infect Dis, Anchorage, AK 99508 USA. RP Dudley, JP (reprint author), Leidos Inc, 20201 Century Blvd,Suite 105, Germantown, MD 20874 USA. EM jpdudley@alaska.edu FU Public Health Agency of Canada; Natural Sciences and Engineering Research Council of Canada; National Science Foundation (DEB-Biodiversity, Discovery and Analysis Program) [125810] FX This article is a contribution of the Beringian Coevolution Project and the Integrated Inventories of Biomes of the Arctic, supported by grants from the National Science Foundation (DEB-Biodiversity, Discovery and Analysis Program-125810) to J. A. Cook (University of New Mexico), E. P. Hoberg (U.S. National Parasite Collection), K. E. Galbreath (Northern Michigan University), and E. Dechaine (Western Washington University). Emily Jenkins' contribution was derived in part from research funded by the Public Health Agency of Canada and the Natural Sciences and Engineering Research Council of Canada. The findings and conclusions in this article are those of the authors and do not necessarily represent those of any government agency or funding institution. NR 139 TC 3 Z9 4 U1 49 U2 132 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 EI 1612-9210 J9 ECOHEALTH JI EcoHealth PD DEC PY 2015 VL 12 IS 4 BP 713 EP 725 DI 10.1007/s10393-015-1036-1 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DA2LW UT WOS:000367627300020 PM 26070525 ER PT J AU Juneja, VK Cadavez, V Gonzales-Barron, U Mukhopadhyay, S AF Juneja, Vijay K. Cadavez, Vasco Gonzales-Barron, Ursula Mukhopadhyay, Sudarsan TI Effect of pH, sodium chloride and sodium pyrophosphate on the thermal resistance of Escherichia coli O157:H7 in ground beef SO FOOD RESEARCH INTERNATIONAL LA English DT Letter C1 [Juneja, Vijay K.; Mukhopadhyay, Sudarsan] USDA, Agr Res Serv, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Cadavez, Vasco; Gonzales-Barron, Ursula] Polytechn Inst Braganza, Sch Agr, CIMO Mt Res Ctr, Braganza, Portugal. RP Juneja, VK (reprint author), USDA, Agr Res Serv, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RI Cadavez, Vasco/A-3958-2010 OI Cadavez, Vasco/0000-0002-3077-7414 NR 0 TC 0 Z9 0 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD DEC PY 2015 VL 78 BP 482 EP 482 DI 10.1016/j.foodres.2015.09.036 PG 1 WC Food Science & Technology SC Food Science & Technology GA DA4MU UT WOS:000367774800054 ER PT J AU Erdman, JW Smith, JW Kuchan, MJ Mohn, ES Johnson, EJ Rubakhin, SS Wang, L Sweedler, JV Neuringer, M AF Erdman, John W., Jr. Smith, Joshua W. Kuchan, Matthew J. Mohn, Emily S. Johnson, Elizabeth J. Rubakhin, Stanislav S. Wang, Lin Sweedler, Jonathan V. Neuringer, Martha TI Lutein and Brain Function SO FOODS LA English DT Article ID PIGMENT OPTICAL-DENSITY; IMAGING MASS-SPECTROMETRY; MACULAR CAROTENOID PIGMENT; HUMAN RETINA; NUTRITIONAL MANIPULATION; COGNITIVE FUNCTION; PRIMATE RETINAS; DOCOSAHEXAENOIC ACID; SINGLE CELLS; OLDER-ADULTS C1 [Erdman, John W., Jr.; Smith, Joshua W.] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA. [Kuchan, Matthew J.] Abbott Nutr, Discovery Res, Columbus, OH 43219 USA. [Mohn, Emily S.; Johnson, Elizabeth J.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rubakhin, Stanislav S.; Wang, Lin; Sweedler, Jonathan V.] Univ Illinois, Dept Chem, Urbana, IL 61801 USA. [Rubakhin, Stanislav S.; Wang, Lin; Sweedler, Jonathan V.] Univ Illinois, Beckman Inst, Urbana, IL 61801 USA. [Neuringer, Martha] Oregon Hlth & Sci Univ, Oregon Natl Primate Res Ctr, Beaverton, OR 97006 USA. RP Erdman, JW (reprint author), Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA. EM jwerdman@illinois.edu; jwsmith5@illinois.edu; Matthew.Kuchan@abbott.com; Emily.Mohn@tufts.edu; Elizabeth.johnson@tufts.edu; roubakhi@illinois.edu; linwang@illinois.edu; jsweedle@illinois.edu; neuringe@ohsu.edu RI Sweedler, Jonathan/A-9405-2009; Rubakhin, Stanislav/E-6685-2017; OI Sweedler, Jonathan/0000-0003-3107-9922; Rubakhin, Stanislav/0000-0003-0437-1493; Smith, Joshua/0000-0002-8636-8097 FU NIH HHS [P51 OD011092] NR 80 TC 4 Z9 4 U1 3 U2 6 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2304-8158 J9 FOODS JI Foods PD DEC PY 2015 VL 4 IS 4 BP 547 EP 564 PG 18 WC Food Science & Technology SC Food Science & Technology GA DA4TW UT WOS:000367794900003 PM 26566524 ER PT J AU Vogler, KC Ager, AA Day, MA Jennings, M Bailey, JD AF Vogler, Kevin C. Ager, Alan A. Day, Michelle A. Jennings, Michael Bailey, John D. TI Prioritization of Forest Restoration Projects: Tradeoffs between Wildfire Protection, Ecological Restoration and Economic Objectives SO FORESTS LA English DT Article DE production possibility frontiers; restoration prioritization; ecosystem services; restoration tradeoffs ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; EASTERN OREGON; MANAGEMENT; FIRE; USA; FRAMEWORK; CONSERVATION; SIMULATION; PRINCIPLES AB The implementation of US federal forest restoration programs on national forests is a complex process that requires balancing diverse socioecological goals with project economics. Despite both the large geographic scope and substantial investments in restoration projects, a quantitative decision support framework to locate optimal project areas and examine tradeoffs among alternative restoration strategies is lacking. We developed and demonstrated a new prioritization approach for restoration projects using optimization and the framework of production possibility frontiers. The study area was a 914,657 ha national forest in eastern Oregon, US that was identified as a national priority for restoration with the goal of increasing fire resiliency and sustaining ecosystem services. The results illustrated sharp tradeoffs among the various restoration goals due to weak spatial correlation of forest stressors and provisional ecosystem services. The sharpest tradeoffs were found in simulated projects that addressed either wildfire risk to the urban interface or wildfire hazard, highlighting the challenges associated with meeting both economic and fire protection goals. Understanding the nature of tradeoffs between restoration objectives and communicating them to forest stakeholders will allow forest managers to more effectively design and implement economically feasible restoration projects. C1 [Vogler, Kevin C.; Bailey, John D.] Oregon State Univ, Dept Forest Engn Resources & Management, Coll Forestry, Corvallis, OR 97331 USA. [Ager, Alan A.] USDA, Forest Serv, Rocky Mt Res Stn, Pendleton, OR 97801 USA. [Day, Michelle A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Coll Forestry, Corvallis, OR 97331 USA. [Jennings, Michael] USDA, Forest Serv, La Grande Forestry & Range Sci Lab, La Grande, OR 97850 USA. RP Vogler, KC (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Coll Forestry, 043 Peavy Hall, Corvallis, OR 97331 USA. EM kevin.vogler@oregonstate.edu; aager@fs.fed.us; michelle.day@oregonstate.edu; michaeldjennings@fs.fed.us; john.bailey@oregonstate.edu OI Vogler, Kevin/0000-0002-7080-2557 FU USDA Forest Service, Pacific Northwest Region FX We thank Kate Marcille for providing comments and suggestions on an earlier draft. We also thank Ken Bunzel for geospatial assistance, and Stephen Hawkins and Larry Sandoval for their input and local expertise. Stu Britain provided coding and technical support for the LTD model. This study was supported by funding from the USDA Forest Service, Pacific Northwest Region. NR 52 TC 6 Z9 6 U1 11 U2 42 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD DEC PY 2015 VL 6 IS 12 BP 4403 EP 4420 DI 10.3390/f6124375 PG 18 WC Forestry SC Forestry GA DA1BS UT WOS:000367531900003 ER PT J AU Altland, JE Morris, L Boldt, J Fisher, P Raudales, R AF Altland, James E. Morris, Leslie Boldt, Jennifer Fisher, Paul Raudales, Rosa TI Sample Container and Storage Temperature for Paclobutrazol Monitoring in Irrigation Water SO HORTTECHNOLOGY LA English DT Article DE greenhouse; nursery; water storage; retention pond; plant growth regulators; sampling methods; triazole AB Padobutrazol is a plant growth retardant commonly used on greenhouse crops. Residues from padobutrazol applications can accumulate in recirculated irrigation water. Given that paclobutrazol has a long half-life and potential biological activity in parts per billion concentrations, it would be desirable to measure padobutrazol concentration in captured irrigation supplies. However, there are no standard protocols for collecting this type of sample. The objective of this research was to determine if sample container material or storage temperature affect padobutrazol stability over time. In two experiments, padobutrazol was mixed in concentrations ranging from 0.04 to 0.2 mg.L-1 and stored in polyethylene, clear glass, or amber glass containers at temperatures of either 4 or 20 degrees C. Padobutrazol concentration was measured at 3, 14, and 30 days after the start of each experiment. Across the two experiments, there were no consistent trends in reduction of padobutrazol concentration with respect to container material or storage temperature. In the first experiment, there was an average of 5% reduction across all treatments from day 0 to 30, whereas in the second experiment, concentration did not decrease over the 30-day time period. These data suggest that padobutrazol is stable in collected water samples for at least 30 days, and that either glass or polyethylene containers are suitable for collecting greenhouse water samples for analysis of padobutrazol concentration. A minimum volume of 100 mL was determined to be the optimum to analyze water samples with diverse padobutrazol concentrations. C1 [Altland, James E.] USDA ARS, Res Horticulturist, Washington, DC 20250 USA. [Fisher, Paul] Univ Florida, Dept Environm Hort, Gainesville, FL 32611 USA. [Raudales, Rosa] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA. RP Altland, JE (reprint author), USDA ARS, Res Horticulturist, Washington, DC 20250 USA. EM james.altland@ars.usda.gov FU Floriculture and Nursery Research Initiative FX This research was funded in part by the Floriculture and Nursery Research Initiative. NR 12 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD DEC PY 2015 VL 25 IS 6 BP 769 EP 773 PG 5 WC Horticulture SC Agriculture GA DA4HK UT WOS:000367760700010 ER PT J AU Albrigo, LG Stover, EW AF Albrigo, Leo G. Stover, Ed W. TI Effect of Plant Growth Regulators and Fungicides on Huanglongbing-related Preharvest Fruit Drop of Citrus SO HORTTECHNOLOGY LA English DT Article DE PGR; GA; 2,4-D; strobilurin fungicides; sweet orange; grapefruit; murcott AB The severe citrus (Citrus sp.) disease Huanglongbing (HLB), associated with Candidatus Liberibacter asiaticus, has resulted in widespread tree decline in Florida and overall citrus production is now the lowest it has been in 50 years. More than 80% of Florida citrus trees are BIB affected, and most growers attempt to sustain production on infected trees through good asian citrus psyllid (Diaphorina citri) control and enhanced fertilization and irrigation management. Although production appears to benefit from these treatments, preharvest fruit drop is considerably greater than on uninfected trees. U.S. Department of Agriculture (USDA) data indicate that Florida statewide fruit drop has increased by 10% to 20% of the entire crop in the last three growing seasons, essentially doubling the historical levels. Extensive research is underway to identify solutions to HLB, but it is essential to maintain production on existing trees to sustain the industry in the near term. For decades, several plant growth regulators (PGRs) have been labeled to reduce preharvest fruit drop in commercial citrus. Trials of these materials, other nonlabeled PGRs, and some fungicides were conducted in two seasons to determine if fruit drop could be reduced. Randomized complete block design experiments were established using four to six replications of four- to six-tree groups as experimental units, blocked spatially. In 2013-14, sprays of gibberellic acid (GA), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), S-abscisic acid (S-ABA), aminoethoxyvinylglycine (AVG), and 1-methykydopropene (1-MCP) were applied once or twice alone or in some combinations at standard rates to trees in various mature blocks of 'Valencia' and 'Pineapple' sweet orange ( Citrus sinensis), 'Star Ruby' grapefruit ( Citrusparadisi), or 'Murcote tangor ( Citrus reticulata x C. sinensis) in central Florida in the Indian River area. Only 1 of the 10 individual trials had treatments with significantly lower drop rates than controls; and when pooled across all experiments, GA + 2,4-D reduced number of fruit dropped per tree 4%, but only at P = 0.10. NAA, S-ABA, AVG, and 1-MCP had no effect and were not used the following year. Starting in 2014, treatments were initiated earlier in the season with greater effort to minimize variability: GA; 2,4-D; GA + 2,4-D; a natural GA, indolebutyric acid, cytokinin mix; and strobilurin fungicides were applied to 22 mature blocks of 'Hamlin' and 'Valencia' sweet orange trees. In 2014-15, only three of the 11 individual 'Hamlin' trials and one of the 11 'Valencia' trials included a treatment with significant drop reduction compared with controls. However, when all the tests on 'Hamlin' were pooled, there was a significant 5% reduction in total crop drop for GA + 2,4-D and significant reductions with many of these PGRs alone, but in only one case with fungicide treatments. When all tests on 'Valencia' were combined, 2,4-D reduced drop significantly but only by 2% of the total crop (14% drop vs. 16% drop), but fruit drop in 'Valencia' blocks was near the historical average in control trees. Soil conditions and tree conditions were similar across all test sites and there were no apparent relationships between product efficacy and observed tree condition or any other grove characteristics. In addition, four 'Hamlin' and four 'Valencia' blocks were treated with 1/ 4 rates of 2,4-D + 6-benzyladenine every 45 days during the growing season (six sprays) and three of the eight individual trials showed significant reductions in drop: when pooled, these treatments reduced drop by 3% in 'Valencia' and 6% in 'Hamlin'. At this time, PGRs cannot be recommended as a consistent way to reduce fruit drop related to BIB, but further work needs to be conducted to refine the most promising treatments. C1 [Albrigo, Leo G.] Univ Florida, Citrus Res & Educ Ctr, IFAS, Lake Alfred, FL 33850 USA. [Stover, Ed W.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Albrigo, LG (reprint author), Univ Florida, Citrus Res & Educ Ctr, IFAS, Lake Alfred, FL 33850 USA. EM albrigo@ufl.edu NR 9 TC 0 Z9 0 U1 4 U2 15 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD DEC PY 2015 VL 25 IS 6 BP 785 EP 790 PG 6 WC Horticulture SC Agriculture GA DA4HK UT WOS:000367760700012 ER PT J AU Dikmen, S Wang, XZ Ortega, MS Cole, JB Null, DJ Hansen, PJ AF Dikmen, S. Wang, X. -z. Ortega, M. S. Cole, J. B. Null, D. J. Hansen, P. J. TI Single nucleotide polymorphisms associated with thermoregulation in lactating dairy cows exposed to heat stress SO JOURNAL OF ANIMAL BREEDING AND GENETICS LA English DT Article DE allele; cattle; heat stress; Single nucleotide polymorphism; thermotolerance ID RECTAL TEMPERATURE; PRODUCTION TRAITS; TROPICAL CLIMATE; CANDIDATE GENES; MILK-PRODUCTION; THERMAL-STRESS; CATTLE; HOLSTEIN; IDENTIFICATION; SOMATOTROPIN AB Dairy cows with increased rectal temperature experience lower milk yield and fertility. Rectal temperature during heat stress is heritable, so genetic selection for body temperature regulation could reduce effects of heat stress on production. One aim of the study was to validate the relationship between genotype and heat tolerance for single nucleotide polymorphisms (SNPs) previously associated with resistance to heat stress. A second aim was to identify new SNPs associated with heat stress resistance. Thermotolerance was assessed in lactating Holsteins during the summer by measuring rectal temperature (a direct measurement of body temperature regulation; n = 435), respiration rate (an indirect measurement of body temperature regulation, n = 450) and sweating rate (the major evaporative cooling mechanism in cattle, n = 455). The association between genotype and thermotolerance was evaluated for 19 SNPs previously associated with rectal temperature from a genomewide analysis study (GWAS), four SNPs previously associated with change in milk yield during heat stress from GWAS, 2 candidate gene SNPs previously associated with rectal temperature and respiration rate during heat stress (ATPA1A and HSP70A) and 66 SNPs in genes previously shown to be associated with reproduction, production or health traits in Holsteins. For SNPs previously associated with heat tolerance, regions of BTA4, BTA6 and BTA24 were associated with rectal temperature; regions of BTA6 and BTA24 were associated with respiration rate; and regions of BTA5, BTA26 and BTA29 were associated with sweating rate. New SNPs were identified for rectal temperature (n = 12), respiration rate (n = 8) and sweating rate (n = 3) from among those previously associated with production, reproduction or health traits. The SNP that explained the most variation were PGR and ASL for rectal temperature, ACAT2 and HSD17B7 for respiration rate, and ARL6IP1 and SERPINE2 for sweating rate. ARL6IP1 was associated with all three thermotolerance traits. In conclusion, specific genetic markers responsible for genetic variation in thermoregulation during heat stress in Holsteins were identified. These markers may prove useful in genetic selection for heat tolerance in Holstein cattle. C1 [Dikmen, S.] Uludag Univ, Fac Vet Med, Dept Anim Sci, Bursa, Turkey. [Wang, X. -z.] Southwest Univ, Coll Anim Sci & Technol, Chongqing, Peoples R China. [Ortega, M. S.; Hansen, P. J.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Cole, J. B.; Null, D. J.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA. RP Hansen, PJ (reprint author), Univ Florida, Dept Anim Sci, POB 110910, Gainesville, FL 32611 USA. EM hansen@animal.ufl.edu RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2010-85122-20623, 2013-68004-20365]; Southeast Milk Inc. FX Research was supported by Agriculture and Food Research Initiative Competitive Grants No. 2010-85122-20623 and 2013-68004-20365 from the USDA National Institute of Food and Agriculture and a grant from the Southeast Milk Inc. Milk Checkoff Program. The authors thank GeneSeek for cooperation in completion of the study and Candace Webb, James Moss, Veronica Negron Perez, Paula Tribulo and Adriana Zolini for assistance with data collection. NR 32 TC 2 Z9 2 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2668 EI 1439-0388 J9 J ANIM BREED GENET JI J. Anim. Breed. Genet. PD DEC PY 2015 VL 132 IS 6 BP 409 EP 419 DI 10.1111/jbg.12176 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DA3FP UT WOS:000367682700002 PM 26198991 ER PT J AU Brown, TC Morrison, MD Benfield, JA Rainbolt, GN Bell, PA AF Brown, Thomas C. Morrison, Mark D. Benfield, Jacob A. Rainbolt, Gretchen Nurse Bell, Paul A. TI Exchange asymmetry in experimental settings SO JOURNAL OF ECONOMIC BEHAVIOR & ORGANIZATION LA English DT Article DE Loss aversion; Endowment effect; Herding; Preference indifference; Incomplete preferences; Regret ID REFERENCE-DEPENDENT PREFERENCES; PROSPECT-THEORY; INDIFFERENCE CURVES; EXPERIMENTAL TESTS; MARKET EXPERIENCE; SOCIAL-INFLUENCE; ENDOWMENT; VALUATION; EXPECTATIONS; DECISION AB We review past trading experiments and present 11 new experiments designed to show how the trading rate responds to alterations of the experimental procedure. In agreement with earlier studies, results show that if the trade decision is converted to one resembling a choice between goods the exchange asymmetry disappears, but otherwise the asymmetry is remarkably robust. Results also indicate that when trading is public herding can occur, which may have caused some of the more extreme examples of the exchange asymmetry, and that some of the lack of trade may result from preference indifference. Nevertheless, some form of status quo bias, which may consist of a combination of loss aversion, gain attraction, regret avoidance, and dislike of trading, and which may be enhanced by indifference between the goods offered, probably remains as an important influence on the trading rate. Published by Elsevier B.V. C1 [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. [Morrison, Mark D.] Charles Sturt Univ, Fac Business, Bathurst, NSW, Australia. [Benfield, Jacob A.] Penn State Univ, Dept Psychol & Social Sci, Abington, PA USA. [Rainbolt, Gretchen Nurse; Bell, Paul A.] Colorado State Univ, Dept Psychol, Ft Collins, CO 80523 USA. RP Brown, TC (reprint author), Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80526 USA. EM thomas.brown@colostate.edu; mmorrison@csu.edu.au; jab908@psu.edu; gretchen402@gmail.com; paul.bell@colostate.edu NR 33 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-2681 EI 1879-1751 J9 J ECON BEHAV ORGAN JI J. Econ. Behav. Organ. PD DEC PY 2015 VL 120 BP 104 EP 116 DI 10.1016/j.jebo.2015.09.006 PG 13 WC Economics SC Business & Economics GA DA0LJ UT WOS:000367488300007 ER PT J AU Yendrek, CR Koester, RP Ainsworth, EA AF Yendrek, Craig R. Koester, Robert P. Ainsworth, Elizabeth A. TI A comparative analysis of transcriptomic, biochemical, and physiological responses to elevated ozone identifies species-specific mechanisms of resilience in legume crops SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Ascorbate-glutathione cycle; air pollution; Glycine max; Phaseolus vulgaris; photosynthesis; Pisum sativum; RNA-Seq ID OXIDATIVE STRESS; SOYBEAN CULTIVARS; ASCORBIC-ACID; ANTIOXIDANT CAPACITY; GLUTATHIONE-REDUCTASE; TRANSGENIC TOBACCO; ABIOTIC STRESSES; CARBON-DIOXIDE; AMBIENT OZONE; PLANTS AB Current concentrations of tropospheric ozone ([O-3]) pollution negatively impact plant metabolism, which can result in decreased crop yields. Interspecific variation in the physiological response of plants to elevated [O-3] exists; however, the underlying cellular responses explaining species-specific differences are largely unknown. Here, a physiological screen has been performed on multiple varieties of legume species. Three varieties of garden pea (Pisum sativum L.) were resilient to elevated [O-3]. Garden pea showed no change in photosynthetic capacity or leaf longevity when exposed to elevated [O-3], in contrast to varieties of soybean (Glycine max (L.) Merr.) and common bean (Phaseolus vulgaris L.). Global transcriptomic and targeted biochemical analyses were then done to examine the mechanistic differences in legume responses to elevated [O-3]. In all three species, there was an O-3-mediated reduction in specific leaf weight and total non-structural carbohydrate content, as well as increased abundance of respiration-related transcripts. Differences specific to garden pea included a pronounced increase in the abundance of GLUTATHIONE REDUCTASE transcript, as well as greater contents of foliar glutathione, apoplastic ascorbate, and sucrose in elevated [O-3]. These results suggest that garden pea may have had greater capacity for detoxification, which prevented net losses in CO2 fixation in an elevated [O-3] environment. C1 [Yendrek, Craig R.; Ainsworth, Elizabeth A.] Univ Illinois, Inst Genom Biol, Urbana, IL 61802 USA. [Koester, Robert P.; Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Ainsworth, Elizabeth A.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Yendrek, CR (reprint author), Univ Illinois, Inst Genom Biol, Urbana, IL 61802 USA. EM Lisa.Ainsworth@ars.usda.gov FU National Soybean Research Laboratory's Soybean Disease Biotechnology Center; United States Department of Agriculture Agricultural Research Service FX The authors would like to thank Sara Kammlade and Chris Montes for technical assistance and Kiran Donthu, Jyothi Thimmapuram, and Courtney Leisner for advice with bioinformatics analysis. We thank Steve Huber for assistance with analysis of glutathionylated proteins. This work was funded by the National Soybean Research Laboratory's Soybean Disease Biotechnology Center and the United States Department of Agriculture Agricultural Research Service. NR 81 TC 3 Z9 3 U1 7 U2 22 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD DEC PY 2015 VL 66 IS 22 BP 7101 EP 7112 DI 10.1093/jxb/erv404 PG 12 WC Plant Sciences SC Plant Sciences GA DA5AR UT WOS:000367815000011 PM 26324463 ER PT J AU Vukovich, M Turner, KL Grazia, TE Mims, T Beasley, JC Kilgo, JC AF Vukovich, Mark Turner, Kelsey L. Grazia, Tracy E. Mims, Thomas Beasley, James C. Kilgo, John C. TI Wintering Golden Eagles on the coastal plain of South Carolina SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE Aquila chrysaetos; camera trap; carcass; open pine forest; southeast; wild pig ID AQUILA-CHRYSAETOS; POPULATION; MODELS; SIZE; LEAD AB Golden Eagles (Aquila chrysaetos) are rare winter residents in eastern North America, with most found along the Appalachian Mountains and few reported on the coastal plain of the Carolinas. We used remote cameras baited with wild pig (Sus scrofa) and white-tailed deer (Odocoileus virginianus) carcasses to detect, age, and individually identify Golden Eagles on the U.S. Department of Energy's Savannah River Site on the coastal plain of South Carolina. We identified eight individual Golden Eagles during the winters of 2013-2014 and 2014-2015, with one detected during both winters. We detected eagles for 19 and 66 calendar days during the winters of 2013-2014 and 2014-2015, respectively, with two adult eagles detected for 30 and 31 calendar days in 2014-2015. Eagles typically scavenged on carcasses for a few days, left, and then returned when cameras were baited with another carcass, suggesting they had remained in the area. These observations suggest that large tracts of forests on the coastal plain may be important wintering areas for some Golden Eagles and, further, that other areas in the coastal plain of the southeastern United States may also harbor wintering eagles. Identification of wintering areas of Golden Eagles in the east will be an important step in the conservation of this protected species, and camera traps baited with carcasses can be an effective tool for such work. C1 [Vukovich, Mark; Kilgo, John C.] USDA Forest Serv, Southern Res Stn, New Ellenton, SC 29809 USA. [Turner, Kelsey L.; Beasley, James C.] Univ Georgia, Savannah River Ecol Lab, Warnell Sch Forestry & Nat Resources, Aiken, SC 29802 USA. [Grazia, Tracy E.; Mims, Thomas] USDA Forest Serv Savannah River, New Ellenton, SC 29809 USA. RP Vukovich, M (reprint author), USDA Forest Serv, Southern Res Stn, POB 700, New Ellenton, SC 29809 USA. EM mvukovich@fs.fed.us FU United States Department of Energy - Savannah River Operations Office through the USDA Forest Service - Savannah River [DE-AI09-00SR22188, DE-FC09-07SR22506] FX We thank R. Hamilton, D. Hammett, and J. Nance for outstanding work in the field, and P. Johns for supplying road-killed carcasses. We also thank C. Sutton and B. Wheeler for assistance with individual identification and ageing of Golden Eagles, D. Forsythe for facilitating identification, and T. Katzner and J. Petranka for reviewing the manuscript. Funding was provided by the United States Department of Energy - Savannah River Operations Office through the USDA Forest Service - Savannah River under Interagency Agreement DE-AI09-00SR22188 and Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. NR 27 TC 0 Z9 0 U1 3 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 EI 1557-9263 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD DEC PY 2015 VL 86 IS 4 BP 337 EP 344 DI 10.1111/jofo.12127 PG 8 WC Ornithology SC Zoology GA DA3AB UT WOS:000367667200007 ER PT J AU Khrimian, A Shirali, S Guzman, F AF Khrimian, Ashot Shirali, Shyam Guzman, Filadelfo TI Absolute Configurations of Zingiberenols Isolated from Ginger (Zingiber officinale) Rhizomes SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID AGGREGATION PHEROMONE; MURGANTIA-HISTRIONICA; HARLEQUIN BUG; ESSENTIAL OIL; STINK BUG; VOLATILE CONSTITUENTS; IDENTIFICATION; ANTIOXIDANT; EXTRACT; GC AB Two stereoisomeric zingiberenols in ginger were identified as (3R,6R,7S)-1,10-bisaboladien-3-ol (2) and (3S,6R,7S)-1,10-bisaboladien-3-ol (5). Absolute configurations were assigned by utilizing 1,10-bisaboladien-3-ol stereoisomers and two gas-chromatography columns: a 25 m Hydrodex-beta-6TBDM and 60 m DB-5MS. The C-6 and C-7 absolute configurations in both zingiberenols match those of zingiberene present abundantly in ginger rhizomes. Interestingly, zingiberenol 2 has recently been identified as a male-produced sex pheromone of the rice stink bug, Oebalus poecilus, thus indicating that ginger plants may be a potential source of the sex pheromone of this bug. C1 [Khrimian, Ashot; Shirali, Shyam; Guzman, Filadelfo] USDA, Invas Insect Biocontrol & Behav Lab, Agr Res Serv, Northeast Area, Beltsville, MD 20705 USA. RP Khrimian, A (reprint author), USDA, Invas Insect Biocontrol & Behav Lab, Agr Res Serv, Northeast Area, Bldg 007,Rm 301,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM khrimian@ars.usda.gov FU USDA NIFA SCRI grant [2011-51181-30937] FX A.K. and S.S. received funding from USDA NIFA SCRI grant #2011-51181-30937. Mention of trade names or commercial products in this publication is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 26 TC 0 Z9 0 U1 4 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD DEC PY 2015 VL 78 IS 12 BP 3071 EP 3074 DI 10.1021/acs.jnatprod.5b00638 PG 4 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA DA1NN UT WOS:000367562600026 PM 26606508 ER PT J AU Sullivan, EM Moon, RJ Kalaitzidou, K AF Sullivan, Erin M. Moon, Robert J. Kalaitzidou, Kyriaki TI Processing and Characterization of Cellulose Nanocrystals/Polylactic Acid Nanocomposite Films SO MATERIALS LA English DT Article DE cellulose nanocrystals; polylactic acid; nanocomposite film ID MECHANICAL-PROPERTIES; CRYSTALLIZATION BEHAVIOR; COMPOSITES; POLYLACTIDE; MORPHOLOGY; PLA; FIBERS AB The focus of this study is to examine the effect of cellulose nanocrystals (CNC) on the properties of polylactic acid (PLA) films. The films are fabricated via melt compounding and melt fiber spinning followed by compression molding. Film fracture morphology, thermal properties, crystallization behavior, thermo-mechanical behavior, and mechanical behavior were determined as a function of CNC content using scanning electron microscopy, differential scanning calorimetry, X-ray diffraction, dynamic mechanical analysis, and tensile testing. Film crystallinity increases with increasing CNC content indicating CNC act as nucleating agents, promoting crystallization. Furthermore, the addition of CNC increased the film storage modulus and slightly broadened the glass transition region. C1 [Sullivan, Erin M.; Moon, Robert J.; Kalaitzidou, Kyriaki] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Kalaitzidou, Kyriaki] Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA. RP Kalaitzidou, K (reprint author), Georgia Inst Technol, Sch Mat Sci & Engn, 771 Ferst Dr NW, Atlanta, GA 30332 USA. EM erin.sullivan@gatech.edu; robertmoon@fs.fed.us; kyriaki.kalaitzidou@me.gatech.edu FU Georgia Tech Manufacturing Institute; Jewell Family Fellowship; P3Nano Forestry Endowment, Cytec Engineered Materials FX The authors would like to thank and acknowledge financial support provided through the P3Nano Forestry Endowment, Cytec Engineered Materials, the Georgia Tech Manufacturing Institute, and the Jewell Family Fellowship. The authors would also like to thank the undergraduate research assistant Vanessa Prema from the School of Materials Science & Engineering at Georgia Institute of Technology for helping with the fabrication of the films. NR 36 TC 5 Z9 5 U1 6 U2 22 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1996-1944 J9 MATERIALS JI Materials PD DEC PY 2015 VL 8 IS 12 BP 8106 EP 8116 DI 10.3390/ma8125447 PG 11 WC Materials Science, Multidisciplinary SC Materials Science GA DA2HN UT WOS:000367616000009 ER PT J AU Xie, JL Zhai, XL Hse, CY Shupe, TF Pan, H AF Xie, Jiulong Zhai, Xianglin Hse, Chung Yun Shupe, Todd F. Pan, Hui TI Polyols from Microwave Liquefied Bagasse and Its Application to Rigid Polyurethane Foam SO MATERIALS LA English DT Article DE liquefaction; bagasse; microwave; polyhydric alcohol; polyurethane foams ID ACID-CATALYZED LIQUEFACTION; SUGAR-CANE BAGASSE; ASSISTED LIQUEFACTION; LIGNOCELLULOSIC BIOMASS; WHEAT-STRAW; ETHYLENE-GLYCOL; CORN STOVER; WOOD; PRODUCTS; CELLULOSE AB Bagasse flour (BF) was liquefied using bi-component polyhydric alcohol (PA) as a solvent and phosphoric acid as a catalyst in a microwave reactor. The effect of BF to solvent ratio and reaction temperatures on the liquefaction extent and characteristics of liquefied products were evaluated. The results revealed that almost 75% of the raw bagasse was converted into liquid products within 9 min at 150 degrees C with a BF to solvent ratio of 1/4. The hydroxyl and acid values of the liquefied bagasse (LB) varied with the liquefied conditions. High reaction temperature combining with low BF to solvent ratio resulted in a low hydroxyl number for the LB. The molecular weight and polydispersity of the LB from reactions of 150 degrees C was lower compared to that from 125 degrees C. Rigid polyurethane (PU) foams were prepared from LB and methylene diphenyl diisocyanate (MDI), and the structural, mechanical and thermal properties of the PU foam were evaluated. The PU foams prepared using the LB from high reaction temperature showed better physical and mechanical performance in comparison to those from low reaction temperature. The amount of PA in the LB has the ability of increasing thermal stability of LB-PU foams. The results in this study may provide fundamental information on integrated utilizations of sugarcane bagasse via microwave liquefaction process. C1 [Xie, Jiulong; Zhai, Xianglin; Shupe, Todd F.] Louisiana State Univ, Sch Renewable Nat Resources, Ctr Agr, Baton Rouge, LA 70803 USA. [Hse, Chung Yun] USDA Forest Serv, Southern Res Stn, Pineville, LA 71360 USA. [Pan, Hui] Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. RP Pan, H (reprint author), Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. EM jxie6@lsu.edu; xzhai1@lsu.edu; chse@fs.fed.us; tshupe@agcenter.lsu.edu; hpan@njfu.edu.cn FU Special Fund for Forest Scientific Research in Public Welfare of China [201504602]; Jiangsu Specially-Appointed Professor program of State Minister of Education of Jiangsu Province, P.R. China FX This work has been funded by the Special Fund for Forest Scientific Research in the Public Welfare (201504602) of China and the Jiangsu Specially-Appointed Professor program of the State Minister of Education of Jiangsu Province, P.R. China. NR 42 TC 2 Z9 2 U1 5 U2 20 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1996-1944 J9 MATERIALS JI Materials PD DEC PY 2015 VL 8 IS 12 BP 8496 EP 8509 DI 10.3390/ma8125472 PG 14 WC Materials Science, Multidisciplinary SC Materials Science GA DA2HN UT WOS:000367616000034 ER PT J AU Fu, JS Zhu, YD Yerke, A Wise, ML Johnson, J Chu, YF Sang, SM AF Fu, Junsheng Zhu, Yingdong Yerke, Aaron Wise, Mitchell L. Johnson, Jodee Chu, YiFang Sang, Shengmin TI Oat avenanthramides induce heme oxygenase-1 expression via Nrf2-mediated signaling in HK-2 cells SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Antioxidant; Avenanthramides; HO-1; Nrf2; Oats ID SMOOTH-MUSCLE-CELLS; IN-VITRO; ANTIOXIDANT ACTIVITY; EPITHELIAL-CELLS; ACTIVATION; NRF2; NEPHROTOXICITY; PROLIFERATION; RESISTANCE; POLYPHENOL AB Scope: Numerous studies have shown that avenanthramides (AVAs), unique compounds found in oats, are strong antioxidants, though the mechanism of action remains unclear. Here, we investigated whether AVAs affect heme oxygenase-1 (HO-1) expression through the activation of Nrf2 translocation. Methods and results: We investigated the effects AVA 2c, 2f, and 2p on HK-2 cells, and found that AVAs could significantly increase HO-1 expression in both a dose- and time-dependent manner. Furthermore, we found that AVA-induced HO-1 expression is mediated by Nrf2 translocation. The addition of N-acetylcysteine (NAC), but not specific inhibitors of p38 (SB202190), PI3K (LY294002), and MEK1 (PD098059) attenuated AVA-induced HO-1 expression, demonstrating an important role for reactive oxygen species, but not PI3K or MAPK activation, in activating the HO-1 pathway. Moreover, hydrogenation of the double bond of the functional alpha,beta-unsaturated carbonyl group of AVAs eliminated their effects on HO-1 expression, suggesting that this group is crucial for the antioxidant activity of AVAs. Conclusion: Our results suggest a novel mechanism whereby AVAs exert an antioxidant function on human health. Further investigation of these markers in human is warranted to explore the beneficial health effects of whole grain oat intake. C1 [Fu, Junsheng] Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou, Peoples R China. [Fu, Junsheng; Zhu, Yingdong; Yerke, Aaron; Sang, Shengmin] North Carolina Agr & Tech State Univ, Ctr Excellence Post Harvest Technol, Kannapolis, NC USA. [Wise, Mitchell L.] USDA, Cereal Crops Res Unit, Madison, WI USA. [Johnson, Jodee; Chu, YiFang] PepsiCo R&D Nutr, Quaker Oats Ctr Excellence, Barrington, IL USA. [Sang, Shengmin] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA. RP Sang, SM (reprint author), North Carolina Agr & Tech State Univ, Ctr Excellence Post Harvest Technol, North Carolina Res Campus, Kannapolis, NC USA. EM ssang@ncat.edu FU Quaker Oats Center of Excellence, PepsiCo, Inc.; PepsiCo, Inc. FX This research was partially funded by Quaker Oats Center of Excellence, PepsiCo, Inc. The funding agency, PepsiCo, Inc., has had no influence of the interpretation of data and the final conclusions drawn. NR 35 TC 2 Z9 2 U1 2 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD DEC PY 2015 VL 59 IS 12 BP 2471 EP 2479 DI 10.1002/mnfr.201500250 PG 9 WC Food Science & Technology SC Food Science & Technology GA DA3WJ UT WOS:000367730600011 PM 26331632 ER PT J AU Evans, EW Jacques, PF Dallal, GE Sacheck, J Must, A AF Evans, E. W. Jacques, P. F. Dallal, G. E. Sacheck, J. Must, A. TI The role of eating frequency on relative weight in urban school-age children SO PEDIATRIC OBESITY LA English DT Article DE Body mass index (BMI) z-score; childhood obesity; eating frequency; snacking ID BODY-MASS INDEX; MEAL FREQUENCY; CHILDHOOD OBESITY; UNITED-STATES; ENERGY-INTAKE; ADOLESCENTS; BMI; OVERWEIGHT; PATTERN; CONSUMPTION AB Background: The role of eating frequency on relative weight in childhood is not well understood. Objective: To clarify this relationship by assessing the cross-sectional and prospective relationships of weekday eating frequency with BMI z-score (BMIz) and change in BMIz in a sample of schoolchildren. Methods: Eating frequency, the average number of reported daily eating occasions, was assessed using two weekday 24-h diet recalls. BMIz was measured at baseline, 6 months and 1 year in 155 urban schoolchildren, ages 9-15 years. Multiple linear regression models were used. Results: Cross-sectional analyses at baseline suggest that BMIz was 0.23 units lower for each additional reported eating occasion (regression coefficient = -0.23; 95% confidence interval [CI]: -0.44, -0.07). From baseline to 6 months, BMIz increased by 0.03 units for each additional reported eating occasion (regression coefficient = 0.03; 95% CI: 0.01, 0.05). This relationship was no longer statistically significant at 1 year (regression coefficient = 0.01; 95% CI: -0.01, 0.03). Conclusions: The findings suggest that the relationship of eating frequency with BMIz differs from that of change in BMIz. This difference may be due to methodological deficiencies of cross-sectional studies, challenges of dietary assessment or differences in eating patterns among normal and overweight youth. Controlled trials are needed to further clarify this relationship. C1 [Evans, E. W.] Brown Univ, Sch Med, Weight Control & Diabet Res Ctr, Providence, RI 02912 USA. [Jacques, P. F.; Dallal, G. E.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, P. F.; Dallal, G. E.; Sacheck, J.; Must, A.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Must, A.] Tufts Univ, Sch Med, Boston, MA 02111 USA. RP Evans, EW (reprint author), Weight Control & Diabet Res Ctr, 196 Richmond St, Providence, RI 02903 USA. EM whitney_evans@brown.edu FU NIH [R01HL106160]; Boston Nutrition Obesity Research Center [NIDDK46200]; National Research Service Award for the NIDDK T32 Research Training Program in Nutrition and Chronic Disease (EWE) FX This study was funded, in part, by NIH R01HL106160, NIDDK46200 (Boston Nutrition Obesity Research Center) and a National Research Service Award for the NIDDK T32 Research Training Program in Nutrition and Chronic Disease (EWE). EWE developed the research questions, coordinated and supervised data collection at all four schools, analyzed and interpreted the data and wrote the initial draft of the manuscript. PFJ and GED contributed to the study design, statistical analysis and manuscript preparation. JS was the PI of the Daily D Study and contributed to the study design, helped with manuscript preparation and approved the final draft of the manuscript as written. AM contributed to the study design, data interpretation, manuscript preparation and provided study oversight. All authors approved the final version of the manuscript. The authors would like to acknowledge the contribution of the Daily D Co-I team of Drs. E. Goodman, C. Gordon, C. Economos, V. Chomitz, and M.F. Holick. NR 30 TC 2 Z9 2 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-6310 EI 2047-6302 J9 PEDIATR OBES JI Pediatr. Obes. PD DEC PY 2015 VL 10 IS 6 BP 442 EP 447 DI 10.1111/ijpo.12004 PG 6 WC Pediatrics SC Pediatrics GA DA3AK UT WOS:000367668100007 PM 25565335 ER PT J AU Harris-Shultz, KR Davis, RF Knoll, JE Anderson, W Wang, HL AF Harris-Shultz, Karen R. Davis, Richard F. Knoll, Joseph E. Anderson, William Wang, Hongliang TI Inheritance and Identification of a Major Quantitative Trait Locus (QTL) that Confers Resistance to Meloidogyne incognita and a Novel QTL for Plant Height in Sweet Sorghum SO PHYTOPATHOLOGY LA English DT Article DE dominance ID ROOT-KNOT NEMATODES; NUCLEOTIDE-BINDING; SUGAR-BEET; GENE; L.; POPULATION; PATHOGENS; GENOTYPES; MARKERS; CLONING AB Southern root-knot nematodes (Meloidogyne incognita) are a pest on many economically important row crop and vegetable species and management relies on chemicals, plant resistance, and cultural practices such as crop rotation. Little is known about the inheritance of resistance to M. incognita or the genomic regions associated with resistance in sorghum (Sorghum bicolor). In this study, an F-2 population (n = 130) was developed between the resistant sweet sorghum cultivar 'Honey Drip' and the susceptible sweet cultivar 'Collier'. Each F-2 plant was phenotyped for stalk weight, height, juice Brix, root weight, total eggs, and eggs per gram of root. Strong correlations were observed between eggs per gram of root and total eggs, height and stalk weight, and between two measurements of Brix. Genotyping-by-sequencing was used to generate single nucleotide polymorphism markers. The G-Model, single marker analysis, interval mapping, and composite interval mapping were used to identify a major quantitative trait locus (QTL) on chromosome 3 for total eggs and eggs per gram of root. Furthermore, a new QTL for plant height was also discovered on chromosome 3. Simple sequence repeat markers were developed in the total eggs and eggs per gram of root QTL region and the markers flanking the resistance gene are 4.7 and 2.4 cM away. These markers can be utilized to move the southern root-knot nematode resistance gene from Honey Drip to any sorghum line. C1 [Harris-Shultz, Karen R.; Knoll, Joseph E.; Anderson, William; Wang, Hongliang] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Davis, Richard F.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. RP Harris-Shultz, KR (reprint author), USDA ARS, Crop Genet & Breeding Res Unit, 115 Coastal Way, Tifton, GA 31793 USA. EM Karen.Harris@ars.usda.gov FU USDA-ARS FX We thank D. Boykin, N. Flader, D. Bunphan, D. Clements, S. Pelc, S. Drawdy, and the anonymous reviewers for their reviews and technical help. This project was funded by the USDA-ARS. NR 51 TC 0 Z9 0 U1 10 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD DEC PY 2015 VL 105 IS 12 BP 1522 EP 1528 DI 10.1094/PHYTO-06-15-0136-R PG 7 WC Plant Sciences SC Plant Sciences GA DA2PW UT WOS:000367639300002 PM 26574655 ER PT J AU Zhu, XQ Xiao, CL AF Zhu, X. Q. Xiao, C. L. TI Phylogenetic, Morphological, and Pathogenic Characterization of Alternaria Species Associated with Fruit Rot of Blueberry in California SO PHYTOPATHOLOGY LA English DT Article ID CHINA; AZOXYSTROBIN; TENUISSIMA; PISTACHIO; BLIGHT; FUNGI AB Fruit rot caused by Alternaria spp. is one of the most important factors affecting the postharvest quality and shelf life of blueberry fruit. The aims of this study were to characterize Alternaria isolates using morphological and molecular approaches and test their pathogenicity to blueberry fruit. Alternaria spp. isolates were collected from decayed blueberry fruit in the Central Valley of California during 2012 and 2013. In total, 283 isolates were obtained and five species of Alternaria, including Alternaria alternata, A. tenuissima, A. arborescens, A. infectoria, and A. rosae, were identified based on DNA sequences of the plasma membrane ATPase, Alt a1 and Calmodulin gene regions in combination with morphological characters of the culture and sporulation. Of the 283 isolates, 61.5% were identified as A. alternata, 32.9% were A. arborescens, 5.0% were A. tenuissima, and only one isolate of A. infectoria and one isolate of A. rosae were found. These fungi were able to grow at temperatures from 0 to 35 degrees C, and mycelial growth was arrested at 40 degrees C. Optimal radial growth occurred between 20 to 30 degrees C. Pathogenicity tests showed that all five Alternaria spp. were pathogenic on blueberry fruit at 0, 4, and 20 degrees C, with A. alternata, A. arborescens, and A. tenuissima being the most virulent species, followed by A. infectoria and A. rosae. Previously A. tenuissima has been reported to be the primary cause of Alternaria fruit rot of blueberry worldwide. Our results indicated that the species composition of Alternaria responsible for Alternaria fruit rot in blueberry can be dependent on geographical region. A. alternata, A. arborescens, A. infectoria, and A. rosae are reported for the first time on blueberry in California. This is also the first report of A. infectoria and A. rosae infecting blueberry fruit. C1 [Zhu, X. Q.] China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China. [Zhu, X. Q.; Xiao, C. L.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov FU China Scholarship Council [201206355002] FX The first author thanks the China Scholarship Council for the financial support (201206355002) during the one-year visit in the United States. We thank S. Pelham for collection of samples and isolation of the fungi, D. Margosan for assisting with microscopy, G. Phillips for assisting with molecular work, and T. Michailides for providing Alternaria reference isolates. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 34 TC 1 Z9 1 U1 3 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD DEC PY 2015 VL 105 IS 12 BP 1555 EP 1567 DI 10.1094/PHYTO-05-15-0122-R PG 13 WC Plant Sciences SC Plant Sciences GA DA2PW UT WOS:000367639300005 PM 26267542 ER PT J AU Wallis, CM Munyaneza, JE Chen, J Novy, R Bester, G Buchman, JL Nordgaard, J van Hest, P AF Wallis, C. M. Munyaneza, J. E. Chen, J. Novy, R. Bester, G. Buchman, J. L. Nordgaard, J. van Hest, P. TI 'Candidatus Liberibacter solanacearum' Titers in and Infection Effects on Potato Tuber Chemistry of Promising Germplasm Exhibiting Tolerance to Zebra Chip Disease SO PHYTOPATHOLOGY LA English DT Article DE carbohydrates; phenolics; plant defense responses; terpenoids; tomato-potato psyllid ID COCKERELLI HEMIPTERA TRIOZIDAE; 1ST REPORT; RESISTANCE; TRANSMISSION; INOCULATION; ASSOCIATION; MANAGEMENT; ACRYLAMIDE; SYMPTOMS; IMPACT AB Long-term sustainable management of zebra chip (ZC) disease of potato requires development of tolerant or resistant germplasm. To this end, 283 potato varieties and breeding clones were infected with the ZC putative causal agent 'Candidatus Liberibacter solanacearum' (Lso) by potato psyllid vector inoculations in 2010, 2011, 2012, and 2013. Potato germplasm was then examined for development of fresh and fried ZC symptoms. Over multiple years 29 breeding clones exhibited little to no symptoms in freshly cut tuber slices, and five exhibited little to no symptoms in fried slices. These five presumed tolerant breeding clones were chosen for further screening to determine whether the lack of physiological responses to Lso infection was the cause of observed tolerance. To this end, tuber amino acid, sugar, and phenolic levels were compared between noninfected and Lso-infected plants. The five putative tolerant clones had less dramatic shifts in host physiology following Lso infection than the susceptible Atlantic cultivar. This suggested lack of host responses to Lso infection that result in major changes in tuber biochemistry is a potential mechanism of ZC resistance. However, the susceptible Atlantic cultivar did have consistently greater Lso titers compared with two of the tolerant entries, so for these reductions in Lso pathogen progression also might be a factor. Regardless, lack of host responses could still remain one trait that could be used to aid in selection of ZC-resistant potato varieties, as other tolerant lines had infection levels consistent with susceptible Atlantic cultivar. These results also suggest that germplasm derived from relatives of cultivated potato plants are viable sources of ZC disease resistance. C1 [Wallis, C. M.; Chen, J.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. [Munyaneza, J. E.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Novy, R.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Bester, G.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Bester, G.] Frito Lay Inc, Rhinelander, WI 54501 USA. [Buchman, J. L.; Nordgaard, J.] Black Gold Farms, Grand Forks, ND 58201 USA. [van Hest, P.] Bejo Seeds Inc, Oceano, CA 93445 USA. RP Wallis, CM (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM christopher.wallis@ars.usda.gov FU USDA-NIFA-Specialty Crop Research Initiative [2009-51181-20176]; USDA-NIFA-RAMP [2009-51101-05892]; Frito-Lay Inc.; Texas Department of Agriculture FX We thank B Heilman, V. Sengoda, M. Heidt, F. de la Rosa, J. Dixon, S. Delp, J. Gefre, M. Henning, G. Aguilar, A. Fite, N. Goodell, A. Wallingford, N. Fonseca, and J. Pedraza for their technical help in this work. Special thanks to S. Jansky, USDA-ARS, Madison, WI, from the ARS clones used in this study. The USDA-NIFA-Specialty Crop Research Initiative (grant 2009-51181-20176) and associated Zebra Chip SCRI/Industry-Funded Mini-Grant Program, USDA-NIFA-RAMP (Project 2009-51101-05892), Frito-Lay Inc., and Texas Department of Agriculture funded this research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 34 TC 1 Z9 1 U1 7 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD DEC PY 2015 VL 105 IS 12 BP 1573 EP 1584 DI 10.1094/PHYTO-02-15-0040-R PG 12 WC Plant Sciences SC Plant Sciences GA DA2PW UT WOS:000367639300007 PM 26312966 ER PT J AU Kandel, YR Haudenshield, JS Srour, AY Islam, KT Fakhoury, AM Santos, P Wang, J Chilvers, MI Hartman, GL Malvick, DK Floyd, CM Mueller, DS Leandro, LFS AF Kandel, Yuba R. Haudenshield, James S. Srour, Ali Y. Islam, Kazi Tariqul Fakhoury, Ahmad M. Santos, Patricia Wang, Jie Chilvers, Martin I. Hartman, Glen L. Malvick, Dean K. Floyd, Crystal M. Mueller, Daren S. Leandro, Leonor F. S. TI Multilaboratory Comparison of Quantitative PCR Assays for Detection and Quantification of Fusarium virguliforme from Soybean Roots and Soil SO PHYTOPATHOLOGY LA English DT Article ID SUDDEN-DEATH SYNDROME; F-SP GLYCINES; POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; SYNDROME PATHOGEN; SOUTH-AMERICA; UNITED-STATES; CAUSAL AGENT; 1ST REPORT; RT-PCR AB The ability to accurately detect and quantify Fusarium virguliforme, the cause of sudden death syndrome (SDS) in soybean, in samples such as plant root tissue and soil is extremely valuable for accurate disease diagnoses and to address research questions. Numerous quantitative real-time polymerase chain reaction (qPCR) assays have been developed for this pathogen but their sensitivity and specificity for F. virguliforme have not been compared. In this study, six qPCR assays were compared in five independent laboratories using the same set of DNA samples from fungi, plants, and soil. Multicopy gene-based assays targeting the ribosomal DNA intergenic spacer (IGS) or the mitochondrial small subunit (mtSSU) showed relatively high sensitivity (limit of detection [LOD] = 0.05 to 5 pg) compared with a single-copy gene (FvTox1)-based assay (LOD = 5 to 50 pg). Specificity varied greatly among assays, with the FvTox1 assay ranking the highest (100%) and two IGS assays being slightly less specific (95 to 96%). Another IGS assay targeting four SDS-causing fusaria showed lower specificity (70%), while the two mtSSU assays were lowest (41 and 47%). An IGS-based assay showed consistently highest sensitivity (LOD = 0.05 pg) and specificity and inclusivity above 94% and, thus, is suggested as the most useful qPCR assay for F. virguliforme diagnosis and quantification. However, specificity was also above 94% in two other assays and their selection for diagnostics and research will depend on objectives, samples, and materials used. These results will facilitate both fundamental and disease management research pertinent to SDS. C1 [Kandel, Yuba R.; Mueller, Daren S.; Leandro, Leonor F. S.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Haudenshield, James S.; Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. [Srour, Ali Y.; Islam, Kazi Tariqul; Fakhoury, Ahmad M.] So Illinois Univ, Dept Plant Soil & Ag Syst, Carbondale, IL 62901 USA. [Santos, Patricia] Dept Biochem & Mol Biol, Reno, NV USA. [Wang, Jie; Chilvers, Martin I.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Malvick, Dean K.; Floyd, Crystal M.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. RP Leandro, LFS (reprint author), Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. EM lleandro@iastate.edu FU North Central Soybean Research Program FX Support for this study was partially provided by the North Central Soybean Research Program. We thank G. Munkvold and M. Ellis for providing isolates of F. solani, F oxysporum F. accuminatum, F. sprotrichoides, and F. graminearum; J. Banta at the University of Illinois, Urbana; and C. Anderson and N. Abdelsamad from ISU for assistance in the laboratory. NR 51 TC 2 Z9 2 U1 2 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD DEC PY 2015 VL 105 IS 12 BP 1601 EP 1611 DI 10.1094/PHYTO-04-15-0096-R PG 11 WC Plant Sciences SC Plant Sciences GA DA2PW UT WOS:000367639300010 PM 26368513 ER PT J AU Romanowicz, KJ Kane, ES Potvin, LR Daniels, AL Kolka, RK Lilleskov, EA AF Romanowicz, Karl J. Kane, Evan S. Potvin, Lynette R. Daniels, Aleta L. Kolka, Randall K. Lilleskov, Erik A. TI Understanding drivers of peatland extracellular enzyme activity in the PEATcosm experiment: mixed evidence for enzymic latch hypothesis SO PLANT AND SOIL LA English DT Article DE Peat; Porewater; Climate change; Extracellular enzyme; Ericaceae; Sedge ID ORGANIC-MATTER DECOMPOSITION; ERICOID MYCORRHIZAL FUNGI; PHENOL OXIDASE ACTIVITY; WATER-TABLE; PEROXIDASE-ACTIVITIES; NORTHERN PEATLANDS; MICROBIAL BIOMASS; BOREAL PEATLANDS; PLANT COMMUNITY; LITTER QUALITY AB Our objective was to assess the impacts of water table position and plant functional groups on peatland extracellular enzyme activity (EEA) framed within the context of the enzymic latch hypothesis. We utilized a full factorial experiment with 2 water table (WT) treatments (high and low) and 3 plant functional groups (PFG: Ericaceae, sedge, Ericaceae and sedge unmanipulated) in twenty-four 1 m(3) intact peatland mesocosms. We measured bulk peat and porewater phase oxidative and hydrolytic enzyme activities monthly from June - October 2012. We also measured physical and porewater chemical constituents in tandem to analyze environmental influences on seasonal enzyme activities. No PFG effects on EEA with WT affecting only acid-phosphatase activity in porewater. Strong seasonal dynamics in EEAs overshadowed our manipulations. Analyses indicated phenolic concentrations were influenced by peat redox potential and negatively correlated with phenol oxidase activity as expected from enzymic latch hypothesis. However, no hydrolytic EEA was influenced by total phenolics, but driven largely by seasonal changes in soil temperature and increasing DOC concentrations in porewater. Our results suggest no support for final step in enzymic latch, in which phenolics are posited to regulate hydrolytic EEAs. Mechanisms regulating seasonal influences remain to be elucidated. C1 [Romanowicz, Karl J.; Kane, Evan S.; Daniels, Aleta L.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Kane, Evan S.; Potvin, Lynette R.; Lilleskov, Erik A.] US Forest Serv, USDA, No Res Stn, Houghton, MI 49931 USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Romanowicz, KJ (reprint author), Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. EM kjromano@umich.edu; eskane@mtu.edu; lrpotvin@fs.fed.us; aldaniel@mtu.edu; rkolka@fs.fed.us; elilleskov@fs.fed.us OI Romanowicz, Karl/0000-0001-8732-3272; Potvin, Lynette/0000-0001-5029-6266 FU USDA Forest Service Northern Research Station Climate Change Program; National Science Foundation [DEB-1146149] FX This research was supported primarily by the USDA Forest Service Northern Research Station Climate Change Program and the National Science Foundation (DEB-1146149). The authors would like to thank Carley J. Kratz, L. Jamie Lamit, and John A. Hribljan for their helpful contributions towards this work. We would also like to thank Todd Ontl, Magdalena Wiedermann, and two anonymous reviewers for their helpful comments and suggestions. The authors declare that they have no conflict of interest. NR 90 TC 1 Z9 1 U1 11 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD DEC PY 2015 VL 397 IS 1-2 BP 371 EP 386 DI 10.1007/s11104-015-2746-4 PG 16 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA DA3GC UT WOS:000367684200027 ER PT J AU Sung, J Lee, S Lee, Y Ha, S Song, B Kim, T Waters, BM Krishnan, HB AF Sung, Jwakyung Lee, Suyeon Lee, Yejin Ha, Sangkeun Song, Beomheon Kim, Taewan Waters, Brian M. Krishnan, Hari B. TI Metabolomic profiling from leaves and roots of tomato (Solanum lycopersicum L.) plants grown under nitrogen, phosphorus or potassium-deficient condition SO PLANT SCIENCE LA English DT Article DE Metabolomics; Mineral deficiency; Amino acids; Organic acids; Carbohydrates; Polyamines ID ORGANIC-ACID EXUDATION; ARABIDOPSIS-THALIANA; CARBOHYDRATE-METABOLISM; SECONDARY METABOLISM; BARLEY PLANTS; RICE PLANTS; NITRATE; CARBON; STRESS; NUTRIENT AB Specific metabolic network responses to mineral deficiencies are not well-defined. Here, we conducted a detailed broad-scale identification of metabolic responses of tomato leaves and roots to N, P or K deficiency. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and deficient (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Based on these results, deficiency of any of these three minerals affected energy production and amino acid metabolism. N deficiency generally led to decreased amino acids and organic acids, and increased soluble sugars. P deficiency resulted in increased amino acids and organic acids in roots, and decreased soluble sugars. K deficiency caused accumulation of soluble sugars and amino acids in roots, and decreased organic acids and amino acids in leaves. Notable metabolic pathway alterations included; (1) increased levels of a-ketoglutarate and raffinose family oligosaccharides in N, P or K-deficient tomato roots, and (2) increased putrescine in K-deficient roots. These findings provide new knowledge of metabolic changes in response to mineral deficiencies. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Sung, Jwakyung; Lee, Suyeon; Lee, Yejin; Ha, Sangkeun] Natl Acad Agr Sci, Div Soil & Fertilizer, RDA, Wonju 565851, North Jeolla Pr, South Korea. [Song, Beomheon] Chungbuk Natl Univ, Dept Agron, Cheongju 361763, North Chungcheo, South Korea. [Kim, Taewan] Hankyong Natl Univ, Dept Plant Life & Environm Sci, Anseong 456749, Gyeonggi Provin, South Korea. [Waters, Brian M.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Krishnan, Hari B.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Krishnan, Hari B.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), ARS, Plant Genet Res Unit, USDA, 205 Curtis Hall, Columbia, MO 65211 USA. EM KrishnanH@missouri.edu RI Waters, Brian/H-4805-2015 OI Waters, Brian/0000-0002-1422-238X FU International Collaborative Research project of National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ008596] FX This study was supported financially by a grant from the International Collaborative Research project (PJ008596) of National Academy of Agricultural Science, Rural Development Administration, Republic of Korea. Metabolon, Inc., Research Triangle Park, NC, performed metabolite analysis reported in this study. Product names are necessary to report factually on available data; however, the University of Missouri and the USDA neither guarantees nor warrants the standard of product, and the use of the name by the University of Missouri and the USDA implies no approval of the product to the exclusion of others that may be suitable. NR 78 TC 2 Z9 2 U1 37 U2 82 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD DEC PY 2015 VL 241 BP 55 EP 64 DI 10.1016/j.plantsci.2015.09.027 PG 10 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DA0LB UT WOS:000367487500006 PM 26706058 ER PT J AU Mittal, A Jiang, YW Ritchie, GL Burke, JJ Rock, CD AF Mittal, Amandeep Jiang, Yingwen Ritchie, Glen L. Burke, John J. Rock, Christopher D. TI AtRAV1 and AtRAV2 overexpression in cotton increases fiber length differentially under drought stress and delays flowering SO PLANT SCIENCE LA English DT Article DE Ovule development; Trichome; Florigen; Fiber length; AtRAV ID GOSSYPIUM-HIRSUTUM L.; EPIDERMAL-CELL DIFFERENTIATION; RAV1 TRANSCRIPTION FACTOR; INITIATION-FACTOR 5A; ABSCISIC-ACID; LOCUS-T; LEAF SENESCENCE; UPLAND COTTON; TRICHOME DEVELOPMENT; FUNCTIONAL-ANALYSIS AB There is a longstanding problem of an inverse relationship between cotton fiber qualities versus high yields. To better understand drought stress signaling and adaptation in cotton (Gossypium hirsutum) fiber development, we expressed the Arabidopsis transcription factors RELATED_TO_ABA_INSENSITIVE3/VIVIPAROUS1/(RAV1) and AtRAV2, which encode APETALA2-Basic3 domain proteins shown to repress transcription of FLOWERING_LOCUS_T(FT) and to promote stomatal opening cell-autonomously. In three years of field trials, we show that AtRAV1 and AtRAV2-overexpressing cotton had similar to 5% significantly longer fibers with only marginal decreases in yields under well-watered or drought stress conditions that resulted in 40-60% yield penalties and 3-7% fiber length penalties in control plants. The longer transgenic fibers from drought-stressed transgenics could be spun into yarn which was measurably stronger and more uniform than that from well-watered control fibers. The transgenic AtRAV1 and AtRAV2 lines flowered later and retained bolls at higher nodes, which correlated with repression of endogenous GhFT-Like (FTL) transcript accumulation. Elevated expression early in development of ovules was observed for GhRAV2L, GhMYB25-Like (MYB25L) involved in fiber initiation, and GhMYB2 and GhMYB25 involved in fiber elongation. Altered expression of RAVs controlling critical nodes in developmental and environmental signaling hierarchies has the potential for phenotypic modification of crops. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Mittal, Amandeep; Jiang, Yingwen; Rock, Christopher D.] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Ritchie, Glen L.] Texas Tech Univ, Dept Plant & Soils Sci, Lubbock, TX 79409 USA. [Burke, John J.] USDA ARS, Plant Stress & Germplasm Lab, Lubbock, TX 79415 USA. RP Rock, CD (reprint author), Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. EM amandeepamittal@gmail.com; nevin.jiang@ttu.edu; glen.ritchie@ttu.edu; jburke@lbk.ars.usda.gov; chris.rock@ttu.edu OI , Christopher/0000-0002-2090-0650 FU TTU-USDA International Cotton Research Center; TX State Support Committee of Cotton Incorporated; USDA Ogallala Aquifer Program FX This work was supported by the TTU-USDA International Cotton Research Center, the TX State Support Committee of Cotton Incorporated, and the USDA Ogallala Aquifer Program. The sponsors had no roles in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the article for publication. Competing financial interest: a patent application for this work has been filed by Texas Tech University in the US Patent Office (USPTO: 62089567). A.M, J.J.B., and C.D.R are listed as co-inventors on the application entitled "Transcription factors and method for increased fiber length of cotton". The authors thank DeeDee Laumbauch and Kay McCrary for transformations, Venu Mendu for helpful comments on the manuscript, Jennifer Smith for greenhouse maintenance, Eric Hequet and the TTU-FBRI for lint and yarn analyses, Tyler Painter and Paul Green for tractor/stripper implementations, and Phil Brown for subsurface drip irrigation management. NR 150 TC 1 Z9 2 U1 8 U2 30 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD DEC PY 2015 VL 241 BP 78 EP 95 DI 10.1016/j.plantsci.2015.09.013 PG 18 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DA0LB UT WOS:000367487500009 PM 26706061 ER PT J AU Schmitz, AJ Begcy, K Sarath, G Walia, H AF Schmitz, Aaron J. Begcy, Kevin Sarath, Gautam Walia, Harkamal TI Rice Ovate Family Protein 2 (OFP2) alters hormonal homeostasis and vasculature development SO PLANT SCIENCE LA English DT Article DE Rice; Ovate Family Protein; OFP; Vasculature; Gibberellin; KNOX; BELL ID CELL-WALL FORMATION; OVATE FAMILY PROTEINS; ARABIDOPSIS-THALIANA; CYTOKININ BIOSYNTHESIS; ECTOPIC EXPRESSION; GENE FAMILY; GENOME-WIDE; SUBCELLULAR-LOCALIZATION; GIBBERELLIN METABOLISM; GRAIN WIDTH AB OFP (Ovate Family Protein) is a transcription factor family found only in plants. In dicots, OFPs control fruit shape and secondary cell wall biosynthesis. OFPs are also thought to function through interactions with KNOX and BELL transcription factors. Here, we have functionally characterized OsOFP2, a member of the OFP subgroup associated with regulating fruit shape. OsOFP2 was found to localize to the nucleus and to the cytosol. A putative nuclear export signal was identified within the OVATE domain and was required for the localization of OsOFP2 to distinct cytosolic spots. Rice plants overexpressing OsOFP2 were reduced in height and exhibited altered leaf morphology, seed shape, and positioning of vascular bundles in stems. Transcriptome analysis indicated disruptions of genes associated with vasculature development, lignin biosynthesis, and hormone homeostasis. Reduced expression of the gibberellin biosynthesis gene GA 20-oxidase 7 coincided with lower gibberellin content in OsOFP2 overexpression lines. Also, we found that OsOFP2 was expressed in plant vasculature and determined that putative vascular development KNOX and BELL proteins interact with OsOFP2. KNOX and BELL genes are known to suppress gibberellin biosynthesis through GA20ox gene regulation and can restrict lignin biosynthesis. We propose that O5OFP2 could modulate KNOX-BELL function to control diverse aspects of development including vasculature development. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Schmitz, Aaron J.; Begcy, Kevin; Walia, Harkamal] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Sarath, Gautam] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RP Walia, H (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. EM hwalia2@unl.edu OI Begcy, Kevin/0000-0002-5046-8029 NR 60 TC 1 Z9 1 U1 3 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD DEC PY 2015 VL 241 BP 177 EP 188 DI 10.1016/j.plantsci.2015.10.011 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DA0LB UT WOS:000367487500017 PM 26706069 ER PT J AU Bontemps, S Arias, M Cara, C Dedieu, G Guzzonato, E Hagolle, O Inglada, J Matton, N Morin, D Popescu, R Rabaute, T Savinaud, M Sepulcre, G Valero, S Ahmad, I Begue, A Wu, BF de Abelleyra, D Diarra, A Dupuy, S French, A Akhtar, IU Kussul, N Lebourgeois, V Le Page, M Newby, T Savin, I Veron, SR Koetz, B Defourny, P AF Bontemps, Sophie Arias, Marcela Cara, Cosmin Dedieu, Gerard Guzzonato, Eric Hagolle, Olivier Inglada, Jordi Matton, Nicolas Morin, David Popescu, Ramona Rabaute, Thierry Savinaud, Mickael Sepulcre, Guadalupe Valero, Silvia Ahmad, Ijaz Begue, Agnes Wu, Bingfang de Abelleyra, Diego Diarra, Alhousseine Dupuy, Stephane French, Andrew Akhtar, Ibrar ul Hassan Kussul, Nataliia Lebourgeois, Valentine Le Page, Michel Newby, Terrence Savin, Igor Veron, Santiago R. Koetz, Benjamin Defourny, Pierre TI Building a Data Set over 12 Globally Distributed Sites to Support the Development of Agriculture Monitoring Applications with Sentinel-2 SO REMOTE SENSING LA English DT Article DE agriculture monitoring; satellite time series; in situ data; Sentinel-2; SPOT4 (Take 5); Landsat 8; JECAM; GEOGLAM ID EARTH OBSERVATION REQUIREMENTS; TIME-SERIES; RESOLUTION; FORMOSAT-2; MISSION; LANDSAT; IMAGES; SEASON AB Developing better agricultural monitoring capabilities based on Earth Observation data is critical for strengthening food production information and market transparency. The Sentinel-2 mission has the optimal capacity for regional to global agriculture monitoring in terms of resolution (10-20 meter), revisit frequency (five days) and coverage (global). In this context, the European Space Agency launched in 2014 the "Sentinel-2 for Agriculture" project, which aims to prepare the exploitation of Sentinel-2 data for agriculture monitoring through the development of open source processing chains for relevant products. The project generated an unprecedented dataset, made of Sentinel-2 like time series and in situ data acquired in 2013 over 12 globally distributed sites. Earth Observation time series were mostly built on the SPOT4 (Take 5) data set, which was specifically designed to simulate Sentinel-2. They also included Landsat 8 and RapidEye imagery as complementary data sources. Images were pre-processed to Level 2A and the quality of the resulting time series was assessed. In situ data about cropland, crop type and biophysical variables were shared by site managers, most of them belonging to the Joint Experiment for Crop Assessment and Monitoring network. This data set allowed testing and comparing across sites the methodologies that will be at the core of the future "Sentinel-2 for Agriculture" system. C1 [Bontemps, Sophie; Matton, Nicolas; Sepulcre, Guadalupe; Defourny, Pierre] Catholic Univ Louvain, Earth & Life Inst, B-1348 Louvain La Neuve, Belgium. [Arias, Marcela; Dedieu, Gerard; Hagolle, Olivier; Inglada, Jordi; Morin, David; Valero, Silvia; Le Page, Michel] Univ Toulouse, CNES CNRS IRD UPS, Ctr Etud Spatiales BIOsphere CESBIO, F-31401 Toulouse, France. [Cara, Cosmin; Popescu, Ramona] CS Romania SA, Craiova 200692, Romania. [Guzzonato, Eric; Rabaute, Thierry; Savinaud, Mickael] CS Syst Informat, F-31506 Toulouse, France. [Ahmad, Ijaz; Akhtar, Ibrar ul Hassan] Pakistan Space & Upper Atmosphere Res Commiss, Natl Agr Informat Ctr Directorate, Islamabad 44000, Pakistan. [Begue, Agnes; Dupuy, Stephane; Lebourgeois, Valentine] Maison Teledetect CIRAD UMR TETIS, F-34093 Montpellier, France. [Wu, Bingfang] Chinese Acad Sci, Inst Remote Sensing & Digital Earth, Beijing 100101, Peoples R China. [de Abelleyra, Diego; Veron, Santiago R.] Inst Nacl Tecnol Agr, Inst Clima & Agua, RA-1686 Hurlingham, Argentina. [Diarra, Alhousseine] Univ Cadi Ayyad, Fac Sci Semlalia, Marrakech 40000, Morocco. [Diarra, Alhousseine; Le Page, Michel] Fac Sci Semlalia, Ctr Geber, Lab Mixte Int TREMA, Marrakech 40000, Morocco. [French, Andrew] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Kussul, Nataliia] Natl Acad Sci Ukraine, Space Res Inst, UA-03680 Kiev, Ukraine. [Kussul, Nataliia] State Space Agcy Ukraine, UA-03680 Kiev, Ukraine. [Newby, Terrence] Agr Res Council South Africa, ZA-0001 Pretoria, South Africa. [Savin, Igor] PFUR, VV Dokuchaev Soil Sci Inst, Moscow 119017, Russia. [Koetz, Benjamin] European Space Agcy, European Space Res Inst, I-00044 Rome, Italy. RP Bontemps, S (reprint author), Catholic Univ Louvain, Earth & Life Inst, 2 Croix Sud Bte L7-05-16, B-1348 Louvain La Neuve, Belgium. EM sophie.bontemps@uclouvain.be; marcela.arias@cesbio.cnes.fr; kraftek@c-s.ro; gerard.dedieu@cesbio.cnes.fr; eric.guzzonato@c-s.fr; olivier.hagolle@cesbio.cnes.fr; jordi.inglada@cesbio.eu; nicolas.matton@uclouvain.be; morind@cesbio.cnes.fr; ramona.popescu@c-s.ro; thierry.rabaute@c-s.fr; mickael.savinaud@c-s.fr; guadalupe.sepulcre@outlook.com; silvia.valero@cesbio.cnes.fr; ijazbhutta@hotmail.com; agnes.begue@cirad.fr; wubf@radi.ac.cn; deabelleyra.diego@inta.gob.ar; recofgi@gmail.com; stephane.dupuy@cirad.fr; andrew.french@ars.usda.gov; ibrar.space@gmail.com; nataliia.kussul@gmail.com; valentine.lebourgeois@cirad.fr; michel.lepage@ird.fr; terry@arc.agric.za; savin_iyu@esoil.ru; veron.santiago@inta.gob.ar; benjamin.koetz@esa.int; pierre.defourny@uclouvain.be RI Kussul, Nataliia/N-8649-2014; begue, agnes/A-5718-2011; SAVIN, IGOR/H-8117-2013 OI Kussul, Nataliia/0000-0002-9704-9702; begue, agnes/0000-0002-9289-1052; SAVIN, IGOR/0000-0002-8739-5441 FU Data User Element of the European Space Agency FX This study has been conducted in the frame of the Sentinel-2 for Agriculture project funded by the Data User Element of the European Space Agency. This work has been possible thanks to the SPOT4 (Take 5) time series, which is the main source of the data set presented here. SPOT4 (Take5) data were provided by the Centre National d'Etudes spatiales (CNES, France). Finally, the project acknowledges all site managers for sharing their data from the 2013 field campaign. NR 28 TC 3 Z9 3 U1 8 U2 26 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD DEC PY 2015 VL 7 IS 12 BP 16062 EP 16090 DI 10.3390/rs71215815 PG 29 WC Remote Sensing SC Remote Sensing GA DA1CN UT WOS:000367534000014 ER PT J AU Shapiro, AC Trettin, CC Kuchly, H Alavinapanah, S Bandeira, S AF Shapiro, Aurelie C. Trettin, Carl C. Kuechly, Helga Alavinapanah, Sadroddin Bandeira, Salomao TI The Mangroves of the Zambezi Delta: Increase in Extent Observed via Satellite from 1994 to 2013 SO REMOTE SENSING LA English DT Article DE mangrove mapping; Landsat; monitoring; deforestation; land use change detection; REDD plus; blue carbon; remote sensing ID FORESTS; ECOSYSTEMS AB Mangroves are recognized for their valued ecosystem services provision while having the highest carbon density among forested ecosystems. Yet they are increasingly threatened by deforestation, conversion to agriculture and development, reducing the benefits they provide for local livelihoods, coastal protection and climate change mitigation. Accordingly, accurate estimates of mangrove area and change are fundamental for developing strategies for sustainable use, conservation and Reducing Emissions from Deforestation and Degradation (REDD+). The Zambezi River Delta in Mozambique contains one of the largest mangrove forests in Africa, and deforestation has been reported to be substantial, however these estimates vary widely. We used Landsat imagery from 1994, 2000 and 2013, to estimate a total current mangrove area of 37,034 ha, which is a net increase of 3723 ha over 19 years. The land cover change assessment was also used to provide perspective on ecosystem carbon stocks, showing that the Zambezi Delta mangrove ecosystem acts as a large carbon sink. Our findings reinforce the importance of conducting land cover change assessments using coherent data and analytical models, coupled with field validation. Broader application of our approach could help quantify the rates of natural change from erosion and land aggradation contrasted with anthropogenic causes. C1 [Shapiro, Aurelie C.] World Wide Fund Nat WWF Germany, D-10117 Berlin, Germany. [Trettin, Carl C.] US Forest Serv, Cordesville, SC 29434 USA. [Kuechly, Helga] Luftbild Umwelt Planung LUP GmbH, D-14469 Potsdam, Germany. [Alavinapanah, Sadroddin] Humboldt Univ, Dept Geog, D-12489 Berlin, Germany. [Bandeira, Salomao] Univ Eduardo Mondlane, Dept Ciencias Biol, Maputo 257, Mozambique. RP Shapiro, AC (reprint author), World Wide Fund Nat WWF Germany, Reinhardstr 18, D-10117 Berlin, Germany. EM Aurelie.shapiro@wwf.de; ctrettin@fs.fed.us; helga.kuechly@lup-umwelt.de; s.alavipanah@hu-berlin.de; salomao.bandeira4@gmail.com FU United States Agency for International Development (USAID) Mozambique Mission FX We wish to thank the United States Agency for International Development (USAID) Mozambique Mission for their support through the Mozambique Global Climate Change Sustainable Landscape Program, in collaboration with the National Directorate for Land and Forests of the Government of Mozambique for the recent biomass inventory and map developed using satellite and airborne technologies combined with data collected during several field surveys. The US Forest Service International Programs provided project management support. Additional thanks to the dedicated field validation team: Hugo Mabilana, Semo Mapai and Dorsia Marinela Langa, and coordination from Celia Macamo; and the invaluable logistical support in Zambezi Delta by Italvino Cunat. NR 34 TC 3 Z9 3 U1 12 U2 25 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD DEC PY 2015 VL 7 IS 12 BP 16504 EP 16518 DI 10.3390/rs71215838 PG 15 WC Remote Sensing SC Remote Sensing GA DA1CN UT WOS:000367534000036 ER PT J AU Heindel, JJ Newbold, RR Bucher, JR Camacho, L Delclos, KB Lewis, SM Vanlandingham, M Churchwell, MI Twaddle, NC McLellen, M Chidambaram, M Bryant, M Woodling, K da Costa, GG Ferguson, SA Flaws, J Howard, PC Walker, NJ Zoeller, RT Fostel, J Favaro, C Schug, TT AF Heindel, Jerrold J. Newbold, Retha R. Bucher, John R. Camacho, Luisa Delclos, K. Barry Lewis, Sherry M. Vanlandingham, Michelle Churchwell, Mona I. Twaddle, Nathan C. McLellen, Michelle Chidambaram, Mani Bryant, Matthew Woodling, Kellie da Costa, Goncalo Gamboa Ferguson, Sherry A. Flaws, Jodi Howard, Paul C. Walker, Nigel J. Zoeller, R. Thomas Fostel, Jennifer Favaro, Carolyn Schug, Thaddeus T. TI NIEHS/FDA CLARITY-BPA research program update SO REPRODUCTIVE TOXICOLOGY LA English DT Review DE Bisphenol A; NIEHS; FDA; NTP; CLARITY-BPA; Consortium; Endocrine disruptors ID SPRAGUE-DAWLEY RATS; ESTROGEN-RECEPTOR EXPRESSION; BISPHENOL-A; ETHINYL ESTRADIOL; THYROID-HORMONES; DNA METHYLATION; ORAL-EXPOSURE; CELL BIOLOGY; FEMALE; TOXICITY AB Bisphenol A (BPA) is a chemical used in the production of numerous consumer products resulting in potential daily human exposure to this chemical. The FDA previously evaluated the body of BPA toxicology data and determined that BPA is safe at current exposure levels. Although consistent with the assessment of some other regulatory agencies around the world, this determination of BPA safety continues to be debated in scientific and popular publications, resulting in conflicting messages to the public. Thus, the National Toxicology Program (NTP), National Institute of Environmental Health Sciences (NIEHS), and U.S. Food and Drug Administration (FDA) developed a consortium-based research program to link more effectively a variety of hypothesis-based research investigations and guideline-compliant safety testing with BPA. This collaboration is known as the Consortium Linking Academic and Regulatory Insights on BPA Toxicity (CLARITY-BPA). This paper provides a detailed description of the conduct of the study and a midterm update on progress of the CLARITY-BPA research program. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). C1 [Heindel, Jerrold J.; Schug, Thaddeus T.] NIEHS, NIH, Div Extramural Res & Training, Res Triangle Pk, NC 27709 USA. [Newbold, Retha R.; Bucher, John R.; Walker, Nigel J.; Fostel, Jennifer] NIEHS, NIH, Div Natl Toxicol Program, Res Triangle Pk, NC 27709 USA. [Camacho, Luisa; Delclos, K. Barry; Vanlandingham, Michelle; Churchwell, Mona I.; Twaddle, Nathan C.; McLellen, Michelle; Chidambaram, Mani; Bryant, Matthew; Woodling, Kellie; da Costa, Goncalo Gamboa] USDA, Div Biochem Toxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. [Ferguson, Sherry A.] USDA, Div Neurotoxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. [Flaws, Jodi] Univ Illinois, Dept Comparat Biosci, Urbana, IL 61802 USA. [Lewis, Sherry M.; Howard, Paul C.] USDA, Off Sci Coordinat, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. [Zoeller, R. Thomas] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. [Favaro, Carolyn] NIEHS, Team Vistronix, NTP Comp & User Support, NIH,Div Natl Toxicol Program, Res Triangle Pk, NC 27709 USA. RP Heindel, JJ (reprint author), NIEHS, NIH, Div Extramural Res & Training, POB 12233, Res Triangle Pk, NC 27709 USA. EM heindelj@niehs.nih.gov RI Walker, Nigel/D-6583-2012 OI Walker, Nigel/0000-0002-9111-6855 FU National Toxicology Program; Food and Drug Administration; National Institute of Environmental Health Sciences/National Institutes of Health [FDA IAG: 224-12-0003, NIEHS IAG: AES12013] FX The NCTR portion of the study was conducted under the auspices of the National Toxicology Program and funded by an Interagency agreement (JAG) between the Food and Drug Administration and the National Institute of Environmental Health Sciences/National Institutes of Health (FDA IAG: 224-12-0003; NIEHS IAG: AES12013). We are grateful for the extraordinary efforts of the NCTR Animal Care, Diet Preparation, Information Technology, Microbiology, Pathology, Quality Assurance, and Veterinary Services staffs, the NIP Statistical Support Team of the Division of Bioinformatics and Biostatistics, as well as Ms. Kathy Carroll of the Office of Scientific Coordination, in the planning and conduct of this study. This manuscript was reviewed in accordance with USFDA and NIEHS procedures prior to submission. The opinions expressed in this paper do not necessarily reflect those of the USFDA. NR 34 TC 7 Z9 7 U1 8 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0890-6238 J9 REPROD TOXICOL JI Reprod. Toxicol. PD DEC PY 2015 VL 58 BP 33 EP 44 DI 10.1016/j.reprotox.2015.07.075 PG 12 WC Reproductive Biology; Toxicology SC Reproductive Biology; Toxicology GA DA1MJ UT WOS:000367559600005 PM 26232693 ER PT J AU Zhang, ML Wen, ZB Hao, XL Byalt, VV Sukhorukov, AP Sanderson, SC AF Zhang, Ming-Li Wen, Zhi-Bin Hao, Xiao-Li Byalt, Vyacheslav V. Sukhorukov, Alexander P. Sanderson, Stewart C. TI Taxonomy, phylogenetics and biogeography of Chesneya (Fabaceae), evidenced from data of three sequences, ITS, trnS-trnG, and rbcL SO BIOCHEMICAL SYSTEMATICS AND ECOLOGY LA English DT Article DE Chesneya; Biogeography; Classification; Evolution; Molecular clock; Phylogeny ID DISPERSAL-VICARIANCE ANALYSIS; CHROMOSOME-NUMBERS; GEOGRAPHIC RANGE; TIBETAN PLATEAU; EVOLUTION; INFERENCE; DNA; DIVERSIFICATION; LEGUMINOSAE; CONFIDENCE AB Plants of Central Asia have played a significant role in the origin of floras of Eurasia and the Northern Hemisphere. Chesneya, a small leguminous genus occurring in Central Asia, western Asia, and Tibet, is used to establish phylogenetic relationships and discuss the evolutionary and biogeographical history based on sequence data of ITS and trnS-trnG and rbcL. We employed BEAST Bayesian inference for dating, and S-DIVA, Lagrange and BBM for ancestral area reconstruction. Our results indicate that Chesniella should be a separate genus, while Spongiocarpella should be included in Chesneya. A classification system within Chesneya comprising five sections is presented. The diversification of Chesneya (crown age ca. 16.56 Ma) is speculated to have been associated with Qinghai-Tibetan Plateau (QTP) uplift. The following aridification process resulted in the Pliocene diversification of four sections of Chesneya during 4.8-2.06 Ma. Ancestral area reconstruction indicates the Himalayas is the ancestral area of Chesneya and Chesniella, but within Central Asia, the western lowlands, can be inferred as the cradle of most dispersals. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Ming-Li; Wen, Zhi-Bin; Hao, Xiao-Li] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. [Zhang, Ming-Li] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Byalt, Vyacheslav V.] Russian Acad Sci, Komarov Bot Inst, RU-197376 St Petersburg, Russia. [Sukhorukov, Alexander P.] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119234, Russia. [Sanderson, Stewart C.] USDA, Forest Serv, Intermt Res Stn, Shrub Sci Lab, Provo, UT 84601 USA. RP Zhang, ML (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Urumqi 830011, Peoples R China. EM zhangml@ibcas.ac.cn RI Sukhorukov, Alexander/D-2084-2013 OI Sukhorukov, Alexander/0000-0003-2220-826X FU China National Key Basic Research Program [2014CB954201]; Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences FX We are grateful to the herbaria staffs of the Komarov Botanical Institute, Russian Academy of Sciences (St. Petersburg), Moscow University (Moscow), Main Botanical Garden, Russian Academy of Sciences (Moscow), and Institute of Botany, Chinese Academy of Sciences (Beijing) for our convenience in checking of specimens. Many thanks to Dr. James I. Cohen working at Texas A&M International University for his English improvements to the manuscript, and to Prof. Matt Lavin, Prof. Richard M. K. Saunders, and Dr. Michael D. Pine for their valuable and constructive comments and suggestions to the manuscript. Funding was provided by the China National Key Basic Research Program (2014CB954201), and the Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences. NR 57 TC 0 Z9 0 U1 3 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0305-1978 EI 1873-2925 J9 BIOCHEM SYST ECOL JI Biochem. Syst. Ecol. PD DEC PY 2015 VL 63 BP 80 EP 89 DI 10.1016/j.bse.2015.09.017 PG 10 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DA0KL UT WOS:000367485900014 ER PT J AU Risch, AC Schutz, M Vandegehuchte, ML van der Putten, WH Duyts, H Raschein, U Gwiazdowicz, DJ Busse, MD Page-Dumroese, DS Zimmermann, S AF Risch, Anita C. Schuetz, Martin Vandegehuchte, Martijn L. van der Putten, Wim H. Duyts, Henk Raschein, Ursina Gwiazdowicz, Dariusz J. Busse, Matt D. Page-Dumroese, Deborah S. Zimmermann, Stephan TI Aboveground vertebrate and invertebrate herbivore impact on net N mineralization in subalpine grasslands SO ECOLOGY LA English DT Article DE above-belowground interactions; exclosure types; functionally different herbivores; herbivory; nutrient cycling; plant biomass; plant properties; soil arthropods; soil mites; soil properties; subalpine grasslands; Switzerland ID NATIONAL-PARK; NITROGEN MINERALIZATION; PLANT-PRODUCTION; RED DEER; LINKAGES; ECOSYSTEM; BIOMASS; SOILS; DECOMPOSITION; COMMUNITIES AB Aboveground herbivores have strong effects on grassland nitrogen (N) cycling. They can accelerate or slow down soil net N mineralization depending on ecosystem productivity and grazing intensity. Yet, most studies only consider either ungulates or invertebrate herbivores, but not the combined effect of several functionally different vertebrate and invertebrate herbivore species or guilds. We assessed how a diverse herbivore community affects net N mineralization in subalpine grasslands. By using size-selective fences, we progressively excluded large, medium, and small mammals, as well as invertebrates from two vegetation types, and assessed how the exclosure types (ET) affected net N mineralization. The two vegetation types differed in long-term management (centuries), forage quality, and grazing history and intensity. To gain a more mechanistic understanding of how herbivores affect net N mineralization, we linked mineralization to soil abiotic (temperature; moisture; NO3-, NH4+, and total inorganic N concentrations/pools; C, N, P concentrations; pH; bulk density), soil biotic (microbial biomass; abundance of collembolans, mites, and nematodes) and plant (shoot and root biomass; consumption; plant C, N, and fiber content; plant N pool) properties. Net N mineralization differed between ET, but not between vegetation types. Thus, short-term changes in herbivore community composition and, therefore, in grazing intensity had a stronger effect on net N mineralization than long-term management and grazing history. We found highest N mineralization values when only invertebrates were present, suggesting that mammals had a negative effect on net N mineralization. Of the variables included in our analyses, only mite abundance and aboveground plant biomass explained variation in net N mineralization among ET. Abundances of both mites and leaf-sucking invertebrates were positively correlated with aboveground plant biomass, and biomass increased with progressive exclusion. The negative impact of mammals on net N mineralization may be related partially to (1) differences in the amount of plant material (litter) returned to the belowground subsystem, which induced a positive bottom-up effect on mite abundance, and (2) alterations in the amount and/or distribution of dung, urine, and food waste. Thus, our results clearly show that short-term alterations of the aboveground herbivore community can strongly impact nutrient cycling within ecosystems independent of long-term management and grazing history. C1 [Risch, Anita C.; Schuetz, Martin; Vandegehuchte, Martijn L.; Raschein, Ursina; Zimmermann, Stephan] Swiss Fed Inst Forest Snow & Landscape Res, CH-8903 Birmensdorf, Switzerland. [van der Putten, Wim H.; Duyts, Henk] Netherlands Inst Ecol, NL-6708 PB Wageningen, Netherlands. [van der Putten, Wim H.] Wageningen Univ, Nematol Lab, NL-6700 ES Wageningen, Netherlands. [Raschein, Ursina] Nat Plan, CH-8614 Sulzbach, Switzerland. [Gwiazdowicz, Dariusz J.] Poznan Univ Life Sci, Fac Forestry, PL-60625 Poznan, Poland. [Busse, Matt D.] USDA Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Page-Dumroese, Deborah S.] USDA Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. RP Risch, AC (reprint author), Swiss Fed Inst Forest Snow & Landscape Res, Zuercherstr 111, CH-8903 Birmensdorf, Switzerland. EM anita.risch@wsl.ch RI Risch, Anita/A-9836-2012; van der Putten, Wim/C-3707-2011 OI Risch, Anita/0000-0003-0531-8336; van der Putten, Wim/0000-0002-9341-4442 FU Swiss National Science Foundation [31003A_122009/1, 31003A_140939/1] FX We thank various employees, interns, and volunteers of WSL (Swiss Federal Institute for Forest, Snow and Landscape Research) and the Swiss National Park (SNP) for their help with fence construction, data collection, and laboratory work. We are grateful to the SNP for administrative support. We thank Douglas A. Frank and an anonymous reviewer for critical remarks and constructive comments on previous versions of the manuscript. The study was funded by the Swiss National Science Foundation grant-no 31003A_122009/1 and grant-no 31003A_140939/1. NR 49 TC 3 Z9 3 U1 24 U2 73 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD DEC PY 2015 VL 96 IS 12 BP 3312 EP 3322 DI 10.1890/15-0300.1 PG 11 WC Ecology SC Environmental Sciences & Ecology GA CZ7OE UT WOS:000367287900018 PM 26909436 ER PT J AU Xu, X Polley, HW Hofmockel, K Daneshgar, PP Wilsey, BJ AF Xu, Xia Polley, H. Wayne Hofmockel, Kirsten Daneshgar, Pedram P. Wilsey, Brian J. TI Plant invasions differentially affected by diversity and dominant species in native- and exotic-dominated grasslands SO ECOLOGY AND EVOLUTION LA English DT Article DE Climate change; dominant species; irrigation; precipitation pattern; native and exotic grasslands; phenology; plant invasions; species richness ID POTENTIAL DISTRIBUTION; TALLGRASS PRAIRIE; UNITED-STATES; BIODIVERSITY; COMMUNITIES; STABILITY; PRECIPITATION; PHENOLOGY; ECOSYSTEMS; PRODUCTIVITY AB Plant invasions are an increasingly serious global concern, especially as the climate changes. Here, we explored how plant invasions differed between native- and novel exotic-dominated grasslands with experimental addition of summer precipitation in Texas in 2009. Exotic species greened up earlier than natives by an average of 18days. This was associated with a lower invasion rate early in the growing season compared to native communities. However, invasion rate did not differ significantly between native and exotic communities across all sampling times. The predictors of invasion rate differed between native and exotic communities, with invasion being negatively influenced by species richness in natives and by dominant species in exotics. Interestingly, plant invasions matched the bimodal pattern of precipitation in Temple, Texas, and did not respond to the pulse of precipitation during the summer. Our results suggest that we will need to take different approaches in understanding of invasion between native and exotic grasslands. Moreover, with anticipated increasing variability in precipitation under global climate change, plant invasions may be constrained in their response if the precipitation pulses fall outside the normal growing period of invaders. C1 [Xu, Xia; Hofmockel, Kirsten; Wilsey, Brian J.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Polley, H. Wayne] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Daneshgar, Pedram P.] Monmouth Univ, Dept Biol, West Long Branch, NJ 07764 USA. RP Xu, X (reprint author), Iowa State Univ, Dept Ecol Evolut & Organismal Biol, 251 Bessey Hall, Ames, IA 50011 USA. EM xuxia@iastate.edu OI Wilsey, Brian J./0000-0002-0628-5006 FU National Science Foundation (NSF) [DEB-0639417]; USDA-NIFA [2014-67003-22067] FX The study was financially supported by National Science Foundation (NSF) DEB-0639417 and USDA-NIFA grant 2014-67003-22067. NR 45 TC 1 Z9 1 U1 6 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD DEC PY 2015 VL 5 IS 23 BP 5662 EP 5670 DI 10.1002/ece3.1830 PG 9 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA CZ9QV UT WOS:000367433000020 PM 27069615 ER PT J AU Dangl, GS Mendum, ML Yang, J Walker, MA Preece, JE AF Dangl, Gerald S. Mendum, Mary Lou Yang, Judy Walker, M. Andrew Preece, John E. TI Hybridization of cultivated Vitis vinifera with wild V.californica and V.girdiana in California SO ECOLOGY AND EVOLUTION LA English DT Article DE California wild grape; domesticated plan introduction; genetic diversity; mixed-species ancestry; natural hybrids; plant conservation genetics ID MULTILOCUS GENOTYPE DATA; MICROSATELLITE REPEATS; DNA POLYMORPHISMS; GENETIC-STRUCTURE; GRAPE CULTIVARS; MARKERS; L.; DIFFERENTIATION; INTROGRESSION; POPULATIONS AB Hybridization of introduced domesticates and closely related natives is well documented in annual crops. The widespread introduction of the domesticated grapevine, Vitis vinifera, into California where it overlaps with two native congenerics, with which it is interfertile, provides opportunity to investigate hybridization between woody perennials. Although geographically widespread, the introduction over the past two centuries has been limited to a few elite clonal cultivars, providing a unique opportunity to study the effects of hybridization on the native species. The amount of hybridization with V.vinifera and the genetic diversity of wild-growing Vitis californica and Vitis girdiana were examined using nineteen microsatellite markers. STRUCTURE analysis was used to define hybrid and introgressed individuals and to analyze genetic structure of the native species. FAMOZ software was used to identify which V.vinifera cultivars served as parents of F-1 hybrids. The three species were clearly distinguished by STRUCTURE analysis. Thirty percent of 119V.californica vines were hybrids. The domesticated parent was identified for 16F(1) hybrid vines; the original California cultivar, Mission', was the parent of eight. Backcrosses were also found, showing introgression into subsequent generations. Similar results were obtained for a small sample of V.girdiana. Removing hybrids greatly reduced the genetic variation of the presumed pure species, among which there was essentially no genetic structure. Limited genetic variability indicates the California natives may be threatened by genetic erosion. The discovery of F-1 hybrids of Mission', a cultivar not grown in the areas for similar to 100years, suggests long generation times for wild vines that, often, grow into expansive liana and propagate by layering, all factors that limit recruitment in populations already disjunct by habitat lose. Hermaphroditic flowers and fruit that is more attractive to birds may favor the production of backcross seed and establishment of introgressed individuals. C1 [Dangl, Gerald S.; Yang, Judy] Univ Calif Davis, Fdn Plant Serv, Davis, CA 95616 USA. [Mendum, Mary Lou] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Walker, M. Andrew] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Preece, John E.] Univ Calif Davis, USDA ARS, Natl Clonal Germplasm Repository, Davis, CA 95616 USA. RP Dangl, GS (reprint author), Univ Calif Davis, Fdn Plant Serv, One Shields Ave, Davis, CA 95616 USA. EM gsdangl@ucdavis.edu FU United States Department of Agriculture/Agricultural Research Service [5306-21000-020-00D] FX This work was supported by the United States Department of Agriculture/Agricultural Research Service [research project 5306-21000-020-00D]. NR 53 TC 3 Z9 3 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD DEC PY 2015 VL 5 IS 23 BP 5671 EP 5684 DI 10.1002/ece3.1797 PG 14 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA CZ9QV UT WOS:000367433000021 PM 27069616 ER PT J AU Kitchen, SG Meyer, SE Carlson, SL AF Kitchen, Stanley G. Meyer, Susan E. Carlson, Stephanie L. TI Mechanisms for maintenance of dominance in a nonclonal desert shrub SO ECOSPHERE LA English DT Article DE age structure; blackbrush; Coleogyne ramosissima; Colorado Plateau; dendrochronology; growth rate; life-history strategies; masting; Mojave Desert; seedling bank; stress tolerance ID BLACKBRUSH COLEOGYNE-RAMOSISSIMA; MOJAVE-DESERT; PLANT-COMMUNITIES; SOUTHWESTERN UTAH; SEED-GERMINATION; PERENNIAL PLANTS; COLORADO PLATEAU; SONORAN DESERT; UNITED-STATES; COMPETITION AB Blackbrush (Coleogyne ramosissima: Rosaceae) is a slow-growing, non-clonal shrub that is regionally dominant on xeric, shallow soils in the North American Mojave Desert-Great Basin transition zone and southern Colorado Plateau. Blackbrush seed production is concentrated in mast years, and most seeds are cached and later consumed by heteromyid rodents. Vegetation histories show that blackbrush stands can persist apparently unchanged for over a century. We used dendrochronological techniques to examine plant age distributions, recruitment patterns and growth rates, to ascertain how blackbrush achieves this long-term population stability. Our study addressed the following questions: (1) What is the role of within-clump recruitment in long-term patterns of clump persistence? Do blackbrush clumps accrue new cohorts through time? (2) How does recruitment vary temporally, specifically in relation to years of mast seed production and climate variability? (3) What impact does intra-specific competition have on plant growth rates? To address these questions, we aged stems from 208 clumps in five Mojave Desert and four Colorado Plateau populations. Individual plant age estimates ranged from 3 to 122 years. Clumps comprised of multiple-aged cohorts were ubiquitous. Within clumps, plant and cohort number increased with clump age, suggesting a steady accumulation of new cohorts over time. Clumps in Colorado Plateau populations accumulated cohorts at a significantly faster rate than clumps in Mojave Desert populations. Recruitment occurred in relatively frequent pulses. It was only partially synchronized with mast years, with some seedling establishment following years of low seed production. Individuals that recruited into established clumps averaged half the radial growth rate of individuals that recruited into openings. Blackbrush recruitment is bimodal, with initial colonization of open spaces from rodent caches but with long-term clump persistence a product of periodic, within-clump recruitment of new plants. This dual recruitment strategy provides a mechanism for continued community dominance in an abiotically stressful environment under low levels of disturbance. C1 [Kitchen, Stanley G.; Meyer, Susan E.; Carlson, Stephanie L.] US Forest Serv, USDA, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. RP Kitchen, SG (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. EM skitchen@fs.fed.us NR 68 TC 0 Z9 0 U1 5 U2 17 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 252 DI 10.1890/ES15-00083.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800005 ER PT J AU Parks, SA Miller, C Parisien, MA Holsinger, LM Dobrowski, SZ Abatzoglou, J AF Parks, Sean A. Miller, Carol Parisien, Marc-Andre Holsinger, Lisa M. Dobrowski, Solomon Z. Abatzoglou, John TI Wildland fire deficit and surplus in the western United States, 1984-2012 SO ECOSPHERE LA English DT Article DE climate; fire deficit; fire departure; fire exclusion; fire occurrence; fire suppression; fire surplus; invasive species; protected areas; wilderness; wildland fire ID SOUTHERN SIERRA-NEVADA; PONDEROSA PINE FOREST; CLIMATE-CHANGE; RED BROME; ECOLOGICAL RESTORATION; VEGETATION HISTORY; NORTHERN ROCKIES; RESTORING FIRE; MOJAVE DESERT; GREAT-BASIN AB Wildland fire is an important disturbance agent in the western US and globally. However, the natural role of fire has been disrupted in many regions due to the influence of human activities, which have the potential to either exclude or promote fire, resulting in a "fire deficit'' or "fire surplus'', respectively. In this study, we developed a model of expected area burned for the western US as a function of climate from 1984 to 2012. We then quantified departures from expected area burned to identify geographic regions with fire deficit or surplus. We developed our model of area burned as a function of several climatic variables from reference areas with low human influence; the relationship between climate and fire is strong in these areas. We then quantified the degree of fire deficit or surplus for all areas of the western US as the difference between expected (as predicted with the model) and observed area burned from 1984 to 2012. Results indicate that many forested areas in the western US experienced a fire deficit from 1984 to 2012, likely due to fire exclusion by human activities. We also found that large expanses of non-forested regions experienced a fire surplus, presumably due to introduced annual grasses and the prevalence of anthropogenic ignitions. The heterogeneity in patterns of fire deficit and surplus among ecoregions emphasizes fundamentally different ecosystem sensitivities to human influences and suggests that large-scale adaptation and mitigation strategies will be necessary in order to restore and maintain resilient, healthy, and naturally functioning ecosystems. C1 [Parks, Sean A.; Miller, Carol; Holsinger, Lisa M.] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Parisien, Marc-Andre] Nat Resources Canada, Northern Forestry Ctr, Canadian Forest Serv, Edmonton, AB T5H 3S5, Canada. [Dobrowski, Solomon Z.] Univ Montana, Dept Forest Management, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Abatzoglou, John] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. RP Parks, SA (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. EM sean_parks@fs.fed.us OI Abatzoglou, John/0000-0001-7599-9750 FU Rocky Mountain Research Station FX We thank two anonymous reviewers for thoughtful comments that significantly improved this manuscript. We acknowledge National Fire Plan funding from the Rocky Mountain Research Station. NR 89 TC 9 Z9 9 U1 9 U2 26 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 275 DI 10.1890/ES15-00294.1 PG 13 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800028 ER PT J AU Tempel, DJ Gutierrez, RJ Battles, JJ Fry, DL Su, YJ Guo, QH Reetz, MJ Whitmore, SA Jones, GM Collins, BM Stephens, SL Kelly, M Berigan, WJ Peery, MZ AF Tempel, Douglas J. Gutierrez, R. J. Battles, John J. Fry, Danny L. Su, Yanjun Guo, Qinghua Reetz, Matthew J. Whitmore, Sheila A. Jones, Gavin M. Collins, Brandon M. Stephens, Scott L. Kelly, Maggi Berigan, William J. Peery, M. Zachariah TI Evaluating short- and long-term impacts of fuels treatments and simulated wildfire on an old-forest species SO ECOSPHERE LA English DT Article DE California Spotted Owl; fuels treatment; habitat; Sierra Nevada; Strix occidentalis occidentalis; territory fitness; territory occupancy; wildfire ID CALIFORNIA SPOTTED OWLS; CENTRAL SIERRA-NEVADA; WESTERN UNITED-STATES; HABITAT SELECTION; HOME-RANGE; INDIVIDUAL TREES; SITE OCCUPANCY; CLIMATE-CHANGE; FIRE SEVERITY; LANDSCAPE AB Fuels-reduction treatments are commonly implemented in the western U.S. to reduce the risk of high-severity fire, but they may have negative short-term impacts on species associated with older forests. Therefore, we modeled the effects of a completed fuels-reduction project on fire behavior and California Spotted Owl (Strix occidentalis occidentalis) habitat and demography in the Sierra Nevada to assess the potential short-and long-term trade-offs. We combined field-collected vegetation data and LiDAR data to develop detailed maps of forest structure needed to parameterize our fire and forest-growth models. We simulated wildfires under extreme weather conditions (both with and without fuels treatments), then simulated forest growth 30 years into the future under four combinations of treatment and fire: treated with fire, untreated with fire, treated without fire, and untreated without fire. We compared spotted owl habitat and population parameters under the four scenarios using a habitat suitability index developed from canopy cover and large-tree measurements at nest sites and from previously derived statistical relationships between forest structure and fitness (lambda) and equilibrium occupancy at the territory scale. Treatments had a positive effect on owl nesting habitat and demographic rates up to 30 years after simulated fire, but they had a persistently negative effect throughout the 30-year period in the absence of fire. We conclude that fuels-reduction treatments in the Sierra Nevada may provide long-term benefits to spotted owls if fire occurs under extreme weather conditions, but can have long-term negative effects on owls if fire does not occur. However, we only simulated one fire under the treated and untreated scenarios and therefore had no measures of variation and uncertainty. In addition, the net benefits of fuels treatments on spotted owl habitat and demography depends on the future probability that fire will occur under similar weather and ignition conditions, and such probabilities remain difficult to quantify. Therefore, we recommend a landscape approach that restricts timber harvest within territory core areas of use (similar to 125 ha in size) that contain critical owl nesting and roosting habitat and locates fuels treatments in the surrounding areas to reduce the potential for high-severity fire in territory core areas. C1 [Tempel, Douglas J.; Reetz, Matthew J.; Whitmore, Sheila A.; Jones, Gavin M.; Berigan, William J.; Peery, M. Zachariah] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Gutierrez, R. J.] Univ Minnesota, Dept Fisheries Wildlife & Conservat Biol, St Paul, MN 55108 USA. [Battles, John J.; Fry, Danny L.; Stephens, Scott L.; Kelly, Maggi] Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Su, Yanjun; Guo, Qinghua] Univ Calif, Sch Engn, Sierra Nevada Res Inst, Merced, CA 95343 USA. [Collins, Brandon M.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP Tempel, DJ (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM dtempel@wisc.edu RI Battles, John/G-8233-2012; OI Battles, John/0000-0001-7124-7893; Jones, Gavin/0000-0002-5102-1229 FU University of Wisconsin; University of California; University of Minnesota; University of Minnesota's Agriculture Experiment Station Project [MN-41036]; USDA Forest Service Region 5; USDA Forest Service Pacific Southwest Research Station; US Fish and Wildlife Service; Sierra Nevada Conservancy; California Department of Water Resources; California Department of Fish and Protection FX This paper is SNAMP Publication Number 39. The Sierra Nevada Adaptive Management Project is funded by USDA Forest Service Region 5, USDA Forest Service Pacific Southwest Research Station, US Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Protection, and the Sierra Nevada Conservancy. We thank the Universities of Wisconsin, California, and Minnesota for funding and administrative support and the University of California's Blodgett Forest Research Station for providing field accommodations. R. J. Gutierrez was supported by the University of Minnesota's Agriculture Experiment Station Project MN-41036. We greatly appreciate the hard work of the field technicians who collected the data for this research. NR 69 TC 8 Z9 8 U1 9 U2 31 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 261 DI 10.1890/ES15-00234.1 PG 19 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800014 ER PT J AU Vaughn, NR Asner, GP Giardina, CP AF Vaughn, Nicholas R. Asner, Gregory P. Giardina, Christian P. TI Long-term fragmentation effects on the distribution and dynamics of canopy gaps in a tropical montane forest SO ECOSPHERE LA English DT Article DE Carnegie Airborne Observatory; edge effects; forest degradation; gap-phase dynamics; Hawaii; power-law distribution ID RAIN-FOREST; BRAZILIAN AMAZON; HABITAT FRAGMENTATION; ECOSYSTEM DEVELOPMENT; NEOTROPICAL FOREST; SPECIES RICHNESS; TREE DIVERSITY; WET FOREST; DISTURBANCES; BIODIVERSITY AB Fragmentation alters forest canopy structure through various mechanisms, which in turn drive subsequent changes to biogeochemical processes and biological diversity. Using repeated airborne LiDAR (Light Detection and Ranging) mappings, we investigated the size distribution and dynamics of forest canopy gaps across a topical montane forest landscape in Hawaii naturally fragmented by lava >100 yr ago. The combined effects of long-term isolation were evaluated using edge-proximity patterns in the distribution of gaps identified in year 1 ("existing gaps''). In addition, using the data from year 2, we investigated patterns in the loss of canopy ("new gaps'') and regrowth of canopy ("filled gaps''). The size distributions of the three gap types were modelled using the power-law exponent, lambda. We found that fragmentation has resulted in some large changes in gap dynamics, with both the total area of gaps and the relative proportion of large existing gaps increased with distance to fragment edge. In models of power-law distribution scaling, lambda estimates decreased to asymptotic values within 20 m of fragment edges for existing gaps. The size distributions of new and filled gaps were surprisingly similar. However, the total area of filled gaps was less than that of new gaps, and this difference was greatest near fragment edges. From these results, we conclude that fragmentation may be capable of continuously altering gap-phase dynamics of a forest for more than a century. C1 [Vaughn, Nicholas R.; Asner, Gregory P.] Carnegie Inst Sci, Dept Global Ecol, Stanford, CA 94306 USA. [Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. RP Vaughn, NR (reprint author), Carnegie Inst Sci, Dept Global Ecol, 260 Panama St, Stanford, CA 94306 USA. EM nvaughn@carnegiescience.edu RI Giardina, Christian/C-3120-2011 OI Giardina, Christian/0000-0002-3431-5073 FU John D. and Catherine T. MacArthur Foundation; Carnegie Institution for Science; National Science Foundation [DEB-0715593, DEB-1020412, DEB-1020007, DEB-1019928]; USDA Forest Service Pacific Southwest Research Station FX This study was supported by the John D. and Catherine T. MacArthur Foundation, the endowment of the Carnegie Institution for Science, National Science Foundation grant DEB-0715593 (G. P. Asner), and the USDA Forest Service Pacific Southwest Research Station, as well as support from National Science Foundation grants DEB-1020412 (T. Fukami, C. P. Giardina, G. P. Asner), DEB-1020007 (D. S. Gruner), and DEB-1019928 (D. J. Flaspohler). We thank C. Baldeck, R. Martin and D. Marvin for comments that improved the manuscript. The Carnegie Airborne Observatory is made possible by the Avatar Alliance Foundation, Margaret A. Cargill Foundation, John D. and Catherine T. MacArthur Foundation, Grantham Foundation for the Protection of the Environment, W. M. Keck Foundation, Gordon and Betty Moore Foundation, Mary Anne Nyburg Baker and G. Leonard Baker Jr., and William R. Hearst III. NR 63 TC 1 Z9 1 U1 7 U2 26 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 271 DI 10.1890/ES15-00235.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800024 ER PT J AU Wang, WJ He, HS Thompson, FR Fraser, JS Hanberry, BB Dijak, WD AF Wang, Wen J. He, Hong S. Thompson, Frank R., III Fraser, Jacob S. Hanberry, Brice B. Dijak, William D. TI Importance of succession, harvest, and climate change in determining future composition in US Central Hardwood Forests SO ECOSPHERE LA English DT Article DE assemblage; competition; dispersal; distribution; forest landscape model; LANDIS PRO; LINKAGES II; regional scales; site scales; species importance value ID SPECIES RANGE SHIFTS; LANDSCAPE MODEL; UNITED-STATES; SPATIALLY EXPLICIT; VEGETATION MODEL; HABITAT MODELS; LANDIS PRO; DYNAMICS; BIOMASS; DISTURBANCE AB Most temperate forests in U.S. are recovering from heavy exploitation and are in intermediate successional stages where partial tree harvest is the primary disturbance. Changes in regional forest composition in response to climate change are often predicted for plant functional types using biophysical process models. These models usually simplify the simulation of succession and harvest and may not consider important species-specific demographic processes driving forests changes. We determined the relative importance of succession, harvest, and climate change to forest composition changes in a 125-million ha area of the Central Hardwood Forest Region of U.S. We used a forest landscape modeling approach to project changes in density and basal area of 23 tree species due to succession, harvest, and four climate scenarios from 2000 to 2300. On average, succession, harvest, and climate change explained 78, 17, and 1% of the variation in species importance values (IV) at 2050, respectively, but their contribution changed to 46, 26, and 20% by 2300. Climate change led to substantial increases in the importance of red maple and southern species (e.g., yellow-poplar) and decreases in northern species (e.g., sugar maple) and most of widely distributed species (e.g., white oak). Harvest interacted with climate change and accelerated changes in some species (e.g., increasing southern red oak and decreasing American beech) while ameliorated the changes for others (e.g., increasing red maple and decreasing white ash). Succession was the primary driver of forest composition change over the next 300 years. The effects of harvest on composition were more important than climate change in the short term but climate change became more important than harvest in the long term. Our results show that it is important to model species-specific responses when predicting changes in forest composition and structure in response to succession, harvest, and climate change. C1 [Wang, Wen J.; He, Hong S.; Fraser, Jacob S.; Hanberry, Brice B.] Univ Missouri, Sch Nat Resources, Columbia, MO 65211 USA. [Thompson, Frank R., III; Dijak, William D.] US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP Wang, WJ (reprint author), Univ Missouri, Sch Nat Resources, Columbia, MO 65211 USA. EM heh@missouri.edu FU Climate Change Response Framework; U.S.D.A. Forest Service Northern Research Station and Eastern Region; Department of Interior USGS Northeast Climate Science Center; University of Missouri-Columbia FX We thank Leslie Brandt, Patricia Butler, Chris Swanston, and Stephen Shifley and the Climate Change Response Framework for their encouragement and support of this effort. This project was funded by the U.S.D.A. Forest Service Northern Research Station and Eastern Region, the Department of Interior USGS Northeast Climate Science Center graduate and postdoctoral fellowships, and the University of Missouri-Columbia. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the views of the United States Government. Author contributions: W. J. Wang, H. S. He, and F. R. Thompson conceived the ideas; J. S. Fraser, B. R. Hanberry, and W. D. Dijak contributed and prepared the data; W. J. Wang performed the analyses and led the writing. All authors contributed to revising the text. NR 63 TC 3 Z9 3 U1 9 U2 34 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 277 DI 10.1890/ES15-00238.1 PG 18 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800030 ER PT J AU Wolfe, JD Stouffer, PC Mokross, K Powell, LL Anciaes, MM AF Wolfe, Jared D. Stouffer, Philip C. Mokross, Karl Powell, Luke L. Anciaes, Marina M. TI Island vs. countryside biogeography: an examination of how Amazonian birds respond to forest clearing and fragmentation SO ECOSPHERE LA English DT Article DE Balbina; central Amazon; continuous forest; countryside biogeography; forest fragment; island biogeography; second growth matrix; species richness; tropical bird ID RAIN-FOREST; SECONDARY; AREA; CONSERVATION; ABUNDANCE; MOVEMENT AB Avian diversity in fragmented Amazonian landscapes depends on a balance between extinction and colonization in cleared and disturbed areas. Regenerating forest facilitates bird dispersal within degraded Amazonian landscapes and may tip the balance in favor of persistence in habitat patches. Determining the response of Amazonian birds to fragmentation may be hindered because many species use adjacent second growth matrices thereby limiting the applicability of island biogeography to predict species loss; alternatively, a countryside biogeographic framework to evaluate the value of regenerating forest may be more appropriate. Here, we used point-count and capture data to compare Amazonian bird communities among continuous forest, 100 ha forest fragments with adjacent second growth, young and older second growth plots, and 100 ha forested islands bounded by water, to test the applicability of island biogeography on the mainland and to assess the ecological value of a regenerating matrix. Among foraging guilds, understory insectivores and flocking species were nearly absent on true islands. Fragments surrounded by young second growth were species rich, suggesting that a developing matrix may mitigate extinction associated with fragmentation. Our findings reinforce that true islands are often extinction-driven systems with distinct, depauperate communities. In contrast, succession of bird communities in second growth facilitates recolonization of forest fragments, permitting fragments as small as 100 ha to support bird communities similar to continuous forest. C1 [Wolfe, Jared D.; Stouffer, Philip C.; Mokross, Karl; Powell, Luke L.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Wolfe, Jared D.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redwood Sci Lab, Arcata, CA 95521 USA. [Wolfe, Jared D.] Klamath Bird Observ, Ashland, OR 97520 USA. [Wolfe, Jared D.; Stouffer, Philip C.; Mokross, Karl; Powell, Luke L.] Inst Nacl de Pesquisas da Amazonia, Biol Dynam Forest Fragments Project, BR-69011 Manaus, Amazonas, Brazil. [Anciaes, Marina M.] Inst Nacl de Pesquisas da Amazonia, Coordenacao Pesquisa Ecol, BR-69011 Manaus, Amazonas, Brazil. RP Wolfe, JD (reprint author), Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM jdw@klamathbird.org FU US National Science Foundation [LTREB 0545491]; American Ornithologists' Union FX Thanks to the multitude of banders, assistants and mateiros who helped collect the data. Logistical support from the Biological Dynamics of Forest Fragmentation Project, Brazil's Instituto Nacional de Pesquisas da Amazonia, and the Smithsonian Institution made this research possible. The quality of the manuscript was greatly improved by Maria Uriarte and two anonymous reviewers. This project would not have been possible without the dedicated help of G. N. Klein at REBIO Uatuma. Funding was provided by a US National Science Foundation (LTREB 0545491) grant and American Ornithologists' Union research grant. Thanks to committee members and field collaborators: Bruce Williamson, Robb Brumfield and Goncalo Ferraz. This article was approved for publication by the Director of the Louisiana Agricultural Experimental Station as manuscript no. 2015-241-22665. This is publication 678 of the Biological Dynamics of Forest Fragments Project Technical Series. NR 38 TC 3 Z9 3 U1 19 U2 64 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD DEC PY 2015 VL 6 IS 12 AR 295 DI 10.1890/ES15-00322.1 PG 14 WC Ecology SC Environmental Sciences & Ecology GA CZ7WS UT WOS:000367311800048 ER PT J AU Cooper, WR Horton, DR AF Cooper, W. Rodney Horton, David R. TI Effects of elicitors of host plant defenses on pear psylla, Cacopsylla pyricola SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE systemic acquired resistance; SAR; induced defenses; salicylic acid; phloem feeder; Hemiptera; Psyllidae; Pyrus communis; Rosaceae; Actigard; Employ; ODC ID ACIBENZOLAR-S-METHYL; APHID RESISTANCE; OCTADECANOID PATHWAY; MYZUS-PERSICAE; SALICYLIC-ACID; POTATO APHID; TOMATO; GENE; HOMOPTERA; RESPONSES AB Pear psylla, Cacopsylla pyricola (Foerster) (Hemiptera: Psyllidae), is a key pest of cultivated pear [Pyrus communis L. (Rosaceae)] in North America and Europe. We examined the effects of foliar applications of three commercially available chemical elicitors of host-plant defenses - Actigard (acibenzolar-S-methyl), Employ (harpin protein), andODC (chitosan)-on survival, development, feeding, and egg laying of C. pyricola. All three defense elicitors reduced the number of nymphs present on pear (cvs. Bartlett or D'Anjou) 30 days after releasing 10 adults on the trees. Choice assays showed that females settled and oviposited on untreated trees more often than on trees treated with any of the three defense elicitors. Results of no-choice assays confirmed that the effects of Actigard, Employ, and ODC on C. pyricola were due to activation of systemic plant responses that led to reduced oviposition preference and nymph survival. However, results did not provide evidence that plant responses to elicitors led to reduced nymphal feeding rates or development. Results of our laboratory studies suggest that commercial defense elicitors may be useful in the integrated management of pear psylla once the effects of elicitors at an ecological scale are better understood. C1 [Cooper, W. Rodney; Horton, David R.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Cooper, WR (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU Fresh and Processed Pear Research Committees FX Pauline Anderson and Heather Headrick provided technical assistance. Funding was provided by the Fresh and Processed Pear Research Committees. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 23 TC 1 Z9 1 U1 6 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD DEC PY 2015 VL 157 IS 3 BP 300 EP 306 DI 10.1111/eea.12360 PG 7 WC Entomology SC Entomology GA CZ8LS UT WOS:000367351800006 ER PT J AU Emanuel, RE Buckley, JJ Caldwell, PV McNulty, SG Sun, G AF Emanuel, Ryan E. Buckley, John J. Caldwell, Peter V. McNulty, Steven G. Sun, Ge TI Influence of basin characteristics on the effectiveness and downstream reach of interbasin water transfers: displacing a problem SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE water resources; global change; hydrological cycles; water management; climate ID ECOLOGICAL IMPACTS; RIVER FLOWS; MANAGEMENT; RESOURCES; SCIENCE; WORLD AB Interbasin water transfers are globally important water management strategies, yet little is known about their role in the hydrologic cycle at regional and continental scales. Specifically, there is a dearth of centralized information on transfer locations and characteristics, and few analyses place transfers into a relevant hydrological context. We assessed hydrological characteristics of interbasin transfers (IBTs) in the conterminous US using a nationwide inventory of transfers together with historical climate data and hydrological modeling. Supplying and receiving drainage basins share similar hydroclimatological conditions, suggesting that climatological drivers of water shortages in receiving basins likely have similar effects on supplying basins. This result calls into question the effectiveness of transfers as a strategy to mitigate climate-driven water shortages, as the water shortage may be displaced but not resolved. We also identified hydrologically advantageous and disadvantageous IBTs by comparing the water balances of supplying and receiving basins. Transfer magnitudes did not vary between the two categories, confirming that factors driving individual IBTs, such as patterns of human water demand or engineering constraints, also influence the continental-scale distribution of transfers. Some IBTs impact streamflow for hundreds of kilometers downstream. Transfer magnitude, hydroclimate and organization of downstream river networks mediate downstream impacts, and these impacts have the potential to expand downstream nonlinearly during years of drought. This work sheds new light on IBTs and emphasizes the need for updated inventories and analyses that place IBTs in an appropriate hydrological context. C1 [Emanuel, Ryan E.; Buckley, John J.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Caldwell, Peter V.] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC USA. [McNulty, Steven G.; Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC USA. RP Emanuel, RE (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. EM ryan_emanuel@ncsu.edu RI Emanuel, Ryan/C-3796-2012 OI Emanuel, Ryan/0000-0002-2166-1698 FU USDA Forest Service Eastern Forest Environmental Threat Assessment Center FX The USDA Forest Service Eastern Forest Environmental Threat Assessment Center is responsible for WaSSI and supported J J B during the course of this work. Dr Pat YEH (National University of Singapore) and an anonymous reviewer provided valuable input on an earlier version of this manuscript. Datasets and new geospatial processing tools presented in this work are included in the supplementary materials. NR 27 TC 2 Z9 2 U1 7 U2 16 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD DEC PY 2015 VL 10 IS 12 AR 124005 DI 10.1088/1748-9326/10/12/124005 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CZ7NO UT WOS:000367286300012 ER PT J AU Sullivan, PF Mulvey, RL Brownlee, AH Barrett, TM Pattison, RR AF Sullivan, Patrick F. Mulvey, Robin L. Brownlee, Annalis H. Barrett, Tara M. Pattison, Robert R. TI Warm summer nights and the growth decline of shore pine in Southeast Alaska SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate change; dendrochronology; diurnal temperature range; divergence; Dothistroma; Pinus contorta contorta ID TEMPERATE FOREST; BRITISH-COLUMBIA; YELLOW-CEDAR; DYNAMICS; CLIMATE; VARIABILITY AB Shore pine, which is a subspecies of lodgepole pine, was a widespread and dominant tree species in Southeast Alaska during the early Holocene. At present, the distribution of shore pine in Alaska is restricted to coastal bogs and fens, likely by competition with Sitka spruce and Western hemlock. Monitoring of permanent plots as part of the United States Forest Service Forest Inventory and Analysis program identified a recent loss of shore pine biomass in Southeast Alaska. The apparent loss of shore pine is concerning, because its presence adds a vertical dimension to coastal wetlands, which are the richest plant communities of the coastal temperate rainforest in Alaska. In this study, we examined the shore pine tree-ring record from a newly established plot network throughout Southeast Alaska and explored climate-growth relationships. We found a steep decline in shore pine growth from the early 1960s to the present. Random Forest regression revealed a strong correlation between the decline in shore pine growth and the rise in growing season diurnal minimum air temperature. Warm summer nights, cool daytime temperatures and a reduced diurnal temperature range are associated with greater cloud cover in Southeast Alaska. This suite of conditions could lead to unfavorable tree carbon budgets (reduced daytime photosynthesis and greater nighttime respiration) and/or favor infection by foliar pathogens, such as Dothistroma needle blight, which has recently caused widespread tree mortality on lodgepole pine plantations in British Columbia. Further field study that includes experimental manipulation (e.g., fungicide application) will be necessary to identify the proximal cause(s) of the growth decline. In the meantime, we anticipate continuation of the shore pine growth decline in Southeast Alaska. C1 [Sullivan, Patrick F.; Brownlee, Annalis H.] Univ Alaska Anchorage, Environm & Nat Resources Inst, Anchorage, AK 99508 USA. [Mulvey, Robin L.] US Forest Serv, Forest Hlth Protect State & Private Forestry, USDA, Juneau, AK 99801 USA. [Barrett, Tara M.] US Forest Serv, Pacific NW Res Stn, USDA, Wenatchee, WA 98801 USA. [Pattison, Robert R.] US Forest Serv, Pacific NW Res Stn, USDA, Anchorage, AK 99503 USA. RP Sullivan, PF (reprint author), Univ Alaska Anchorage, Environm & Nat Resources Inst, Anchorage, AK 99508 USA. EM pfsullivan@uaa.alaska.edu FU USDA Pacific Northwest Research Station [PNW 11-JV-112619-17-100]; University of Alaska Anchorage [PNW 11-JV-112619-17-100]; Western Wildlands Environmental Threat Assessment Center FX This project was supported by Joint Venture Agreement #PNW 11-JV-112619-17-100 between the USDA Pacific Northwest Research Station and the University of Alaska Anchorage, with partial funding from the Western Wildlands Environmental Threat Assessment Center. K Nielsen assisted with increment core processing in the laboratory. Installation of the shore pine monitoring network was funded by the United States Forest Service Forest Health Monitoring Program. NR 37 TC 2 Z9 2 U1 4 U2 24 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD DEC PY 2015 VL 10 IS 12 AR 124007 DI 10.1088/1748-9326/10/12/124007 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CZ7NO UT WOS:000367286300014 ER PT J AU Okagaki, LH Nunes, CC Sailsbery, J Clay, B Brown, D John, T Oh, Y Young, N Fitzgerald, M Haas, BJ Zeng, QD Young, S Adiconis, X Fan, L Levin, JZ Mitchell, TK Okubara, PA Farman, ML Kohn, LM Birren, B Ma, LJ Dean, RA AF Okagaki, Laura H. Nunes, Cristiano C. Sailsbery, Joshua Clay, Brent Brown, Doug John, Titus Oh, Yeonyee Young, Nelson Fitzgerald, Michael Haas, Brian J. Zeng, Qiandong Young, Sarah Adiconis, Xian Fan, Lin Levin, Joshua Z. Mitchell, Thomas K. Okubara, Patricia A. Farman, Mark L. Kohn, Linda M. Birren, Bruce Ma, Li-Jun Dean, Ralph A. TI Genome Sequences of Three Phytopathogenic Species of the Magnaporthaceae Family of Fungi SO G3-GENES GENOMES GENETICS LA English DT Article DE Magnaporthe; Gaeumannomyces; sequence; repetitive DNA; synteny ID RICE BLAST FUNGUS; TRANSPOSABLE ELEMENTS; PATHOGENIC FUNGUS; PLANT-PATHOGENS; SMALL RNAS; ORYZAE; EVOLUTION; REVEALS; GRISEA; IMPACT AB Magnaporthaceae is a family of ascomycetes that includes three fungi of great economic importance: Magnaporthe oryzae, Gaeumannomyces graminis var. tritici, and Magnaporthe poae. These three fungi cause widespread disease and loss in cereal and grass crops, including rice blast disease (M. oryzae), take-all disease in wheat and other grasses (G. graminis), and summer patch disease in turf grasses (M. poae). Here, we present the finished genome sequence for M. oryzae and draft sequences for M. poae and G. graminis var. tritici. We used multiple technologies to sequence and annotate the genomes of M. oryzae, M. poae, and G. graminis var. tritici. The M. oryzae genome is now finished to seven chromosomes whereas M. poae and G. graminis var. tritici are sequenced to 40.0x and 25.0x coverage respectively. Gene models were developed by the use of multiple computational techniques and further supported by RNAseq data. In addition, we performed preliminary analysis of genome architecture and repetitive element DNA. C1 [Okagaki, Laura H.; Nunes, Cristiano C.; Sailsbery, Joshua; Clay, Brent; Brown, Doug; John, Titus; Oh, Yeonyee; Dean, Ralph A.] N Carolina State Univ, Ctr Integrated Fungal Res, Raleigh, NC 27606 USA. [Okagaki, Laura H.; Nunes, Cristiano C.; Sailsbery, Joshua; Oh, Yeonyee; Dean, Ralph A.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27606 USA. [Young, Nelson; Ma, Li-Jun] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. [Fitzgerald, Michael; Haas, Brian J.; Zeng, Qiandong; Young, Sarah; Adiconis, Xian; Fan, Lin; Levin, Joshua Z.; Birren, Bruce] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Mitchell, Thomas K.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Okubara, Patricia A.] USDA ARS, Root Dis & Biol Control, Pullman, WA 99164 USA. [Farman, Mark L.] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Kohn, Linda M.] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. RP Dean, RA (reprint author), N Carolina State Univ, Ctr Integrated Fungal Res, Raleigh, NC 27606 USA. EM lijun@biochem.umass.edu; radean2@ncsu.edu FU Tri-institutional Molecular Mycology and Pathogenesis training grant (National Institutes of Health) [5T32-AI052080-09]; National Science Foundation [MCB-0333269]; Current Research Information System Project [5348-22000-012-00D, MCB-0731808] FX We thank Eric Lyons at the University of Arizona for his technical help with GeVo/CoGe. Laura Okagaki was supported by the Tri-institutional Molecular Mycology and Pathogenesis training grant (National Institutes of Health 5T32-AI052080-09). Funding was provided by National Science Foundation MCB-0333269 (Birren and Dean), Current Research Information System Project 5348-22000-012-00D (Okubara), and MCB-0731808 (Dean, Ma, Kohn, Okubara, and Mitchell). NR 35 TC 1 Z9 1 U1 6 U2 18 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD DEC PY 2015 VL 5 IS 12 BP 2539 EP 2545 DI 10.1534/g3.115.020057 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CZ7CM UT WOS:000367257500003 PM 26416668 ER PT J AU Edae, EA Bowden, RL Poland, J AF Edae, Erena A. Bowden, Robert L. Poland, Jesse TI Application of Population Sequencing (POPSEQ) for Ordering and Imputing Genotyping-by-Sequencing Markers in Hexaploid Wheat SO G3-GENES GENOMES GENETICS LA English DT Article DE GBS; WGS; POPSEQ; linkage map; imputation ID SINGLE-NUCLEOTIDE POLYMORPHISMS; TRITICUM-AESTIVUM L.; GENOMIC SELECTION; GENOMEWIDE SELECTION; AEGILOPS-TAUSCHII; MAP; PREDICTION; DISCOVERY; BARLEY; MAIZE AB The advancement of next-generation sequencing technologies in conjunction with new bioinformatics tools enabled fine-tuning of sequence-based, high-resolution mapping strategies for complex genomes. Although genotyping-by-sequencing (GBS) provides a large number of markers, its application for association mapping and genomics-assisted breeding is limited by a large proportion of missing data per marker. For species with a reference genomic sequence, markers can be ordered on the physical map. However, in the absence of reference marker order, the use and imputation of GBS markers is challenging. Here, we demonstrate how the population sequencing (POPSEQ) approach can be used to provide marker context for GBS in wheat. The utility of a POPSEQ-based genetic map as a reference map to create genetically ordered markers on a chromosome for hexaploid wheat was validated by constructing an independent de novo linkage map of GBS markers from a Synthetic W7984 x Opata M85 recombinant inbred line (SynOpRIL) population. The results indicated that there is strong agreement between the independent de novo linkage map and the POPSEQ mapping approach in mapping and ordering GBS markers for hexaploid wheat. After ordering, a large number of GBS markers were imputed, thus providing a high-quality reference map that can be used for QTL mapping for different traits. The POPSEQ-based reference map and whole-genome sequence assemblies are valuable resources that can be used to order GBS markers and enable the application of highly accurate imputation methods to leverage the application GBS markers in wheat. C1 [Edae, Erena A.; Bowden, Robert L.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Edae, Erena A.; Poland, Jesse] Kansas State Univ, Wheat Genet Resource Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA. [Edae, Erena A.; Poland, Jesse] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. RP Edae, EA (reprint author), Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM Erena.Edae@ars.usda.gov; jpoland@ksu.edu OI Poland, Jesse/0000-0002-7856-1399 FU United States Department of Agriculture (USDA) - Agricultural Research Service [3020-21000-010-00D]; National Research Initiative Competitive Grants CAP project from the USDA National Institute of Food and Agriculture [2011-68002-30029]; US Agency for International Development (USAID) [AID-OAA-A-13-0005]; National Science Foundation (NSF) Plant Genome Research Program [IOS-1339389]; Wheat Genetics Resource Center (WGRC) Industry/University Collaborative Research Center (I/UCRC) - NSF [IIP-1338897]; NSF [CNS-1006860, EPS-1006860, EPS-0919443] FX This project is funded by the United States Department of Agriculture (USDA) - Agricultural Research Service (Appropriation #3020-21000-010-00D) and National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, the US Agency for International Development (USAID Cooperative Agreement No. AID-OAA-A-13-0005), and the National Science Foundation (NSF) Plant Genome Research Program (IOS-1339389). This work was completed under the auspices of the Wheat Genetics Resource Center (WGRC) Industry/University Collaborative Research Center (I/UCRC) supported by NSF grant contract (IIP-1338897) and industry partners. Computational resources for this project were through Beocat HPC at Kansas State University, which is funded in part by NSF grants CNS-1006860, EPS-1006860, and EPS-0919443. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. This work represents contribution number 15-459-J from the Kansas Agricultural Experiment Station. NR 39 TC 2 Z9 2 U1 6 U2 24 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD DEC PY 2015 VL 5 IS 12 BP 2547 EP 2553 DI 10.1534/g3.115.020362 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CZ7CM UT WOS:000367257500004 PM 26530417 ER PT J AU Franssen, F Bilska-Zajac, E Deksne, G Sprong, H Pozio, E Rosenthal, B Rozycki, M van der Giessen, J AF Franssen, Frits Bilska-Zajac, Ewa Deksne, Gunita Sprong, Hein Pozio, Edoardo Rosenthal, Benjamin Rozycki, Mirek van der Giessen, Joke TI Genetic evidence of interspecies introgression of mitochondrial genomes between Trichinella spiralis and Trichinella britovi under natural conditions SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Trichinella britovi; Trichinella spiralis; Haplotypes; Natural hybrids; Wild boar; Red fox; Poland ID MIXED INFECTION; IDENTIFICATION; HYBRIDIZATION; EPIDEMIOLOGY; NATIVA; POPULATION; DIVERGENCE; NEMATODES; GENOTYPES; REVEALS AB Trichinellosis is a zoonotic disease caused by Trichinella muscle larvae (ML) through ingestion of raw or undercooked meat. To date, 12 taxa are recognized in this genus, of which four are circulating in Europe (Trichinella spiralis, Trichinella nativa, Trichinella britovi and Trichinella pseudospiralis). T. spiralis and T. britovi circulate in European wildlife and occur simultaneously in the same host species. The possibility of hybrid formation between T. britovi and T. spiralis has hardly been addressed and so far, results of experimental hybridisation attempts between T. britovi and T. spiralis are inconclusive. The aim of the present study was to analyse molecular polymorphisms of single T. spiralis and T. britovi ML from natural infections based on nuclear 5S rDNA intergenic spacer region (5S rDNA-ISR) and mitochondrial cytochrome c oxidase 1 (CO1) gene sequences. Six haplotypes of the 5S rDNA intergenic spacer region (5S rDNA-ISR) and 14 of the cytochrome c oxidase 1 (CO1) gene were demonstrated in 89 individual T. britovi ML from Latvia and Poland. In contrast, only two haplotypes were observed at both 5S rDNA-ISR and CO1 of 57 individual T. spiralis ML from Polish wild boar and red foxes. Moreover, this study demonstrates hybridisation in eight individual ML between T. britovi and T. spiralis under natural conditions in four Polish wild boar and two red foxes, revealed by combining 5S rDNA-ISR and CO1 sequence information of individual Trichinella ML. To our knowledge, this is the first report of interspecies hybridisation between T. spiralis and T. britovi under field conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Franssen, Frits; Sprong, Hein; van der Giessen, Joke] Natl Inst Publ Hlth & Environm RIVM, NL-3721 MA Bilthoven, Netherlands. [Bilska-Zajac, Ewa; Rozycki, Mirek] Natl Vet Res Inst Pulawy PIWet, Pulawy, Poland. [Deksne, Gunita] Inst Food Safety Anim Hlth & Environm BIOR, Riga, Latvia. [Pozio, Edoardo] ISS, Rome, Italy. [Rosenthal, Benjamin] ARS, USDA, Beltsville, MD USA. RP Franssen, F (reprint author), Natl Inst Publ Hlth & Environm RIVM, Antonie van Leeuwenhoeklaan 9, NL-3721 MA Bilthoven, Netherlands. EM frits.franssen@rivm.nl; ewa.bilska@piwet.pulawy.pl; gunita.deksne@bior.lv; hein.sprong@rivm.nl; edoardo.pozio@iss.it; Benjamin.Rosenthal@ARS.USDA.GOV; mrozycki@piwet.pulawy.pl; joke.van.der.giessen@rivm.nl OI Rosenthal, Benjamin/0000-0002-0224-3773; Sprong, Hein/0000-0002-0218-4320 FU European MED-VET-NET Association [STM2013-9]; Dutch Food and Product Safety Authority (NVWA) [V/330310/07/TR]; DG SANCO of the European Commission FX This study was financed by the European MED-VET-NET Association (STM2013-9) and by the Dutch Food and Product Safety Authority (NVWA) (V/330310/07/TR). The Trichinella biobank was in part financed by the DG SANCO of the European Commission from 2006 to 2014. The authors thank Harry Vennema for critically reviewing sequence analyses results. NR 28 TC 1 Z9 2 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD DEC PY 2015 VL 36 BP 323 EP 332 DI 10.1016/j.meegid.2015.10.005 PG 10 WC Infectious Diseases SC Infectious Diseases GA DA1IA UT WOS:000367548300042 PM 26458526 ER PT J AU Paine, TD Millar, JG Hanks, LM Gould, J Wang, Q Daane, K Dahlsten, DL Mcpherson, EG AF Paine, T. D. Millar, J. G. Hanks, L. M. Gould, J. Wang, Q. Daane, K. Dahlsten, D. L. Mcpherson, E. G. TI Cost-Benefit Analysis for Biological Control Programs That Targeted Insect Pests of Eucalypts in Urban Landscapes of California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE classical biological control; street tree; urban landscape; natural enemy; Eucalyptus ID LONGHORNED BORER COLEOPTERA; PARASITOID AVETIANELLA-LONGOI; HYMENOPTERA EULOPHIDAE; PSYLLAEPHAGUS-BLITEUS; GALL WASP; SEMIPUNCTATA; CERAMBYCIDAE; PREFERENCES; ENCYRTIDAE; MANAGEMENT AB As well as being planted for wind breaks, landscape trees, and fuel wood, eucalypts are also widely used as urban street trees in California. They now are besieged by exotic insect herbivores of four different feeding guilds. The objective of the current analysis was to determine the return on investment from biological control programs that have targeted these pests. Independent estimates of the total number of eucalypt street trees in California ranged from a high of 476,527 trees (based on tree inventories from 135 California cities) to a low of 190,666 trees (based on 49 tree inventories). Based on a survey of 3,512 trees, the estimated mean value of an individual eucalypt was US$5,978. Thus, the total value of eucalypt street trees in California ranged from more than US$1.0 billion to more than US$2.8 billion. Biological control programs that targeted pests of eucalypts in California have cost US$2,663,097 in extramural grants and University of California salaries. Consequently, the return derived from protecting the value of this resource through the biological control efforts, per dollar expended, ranged from US$1,070 for the high estimated number of trees to US$428 for the lower estimate. The analyses demonstrate both the tremendous value of urban street trees, and the benefits that stem from successful biological control programs aimed at preserving these trees. Economic analyses such as this, which demonstrate the substantial rates of return from successful biological control of invasive pests, may play a key role in developing both grass-roots and governmental support for future urban biological control efforts. C1 [Paine, T. D.; Millar, J. G.; Hanks, L. M.; Gould, J.; Wang, Q.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Wang, Q.] Massey Univ, Inst Agr & Environm, Palmerston North, New Zealand. [Daane, K.; Dahlsten, D. L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Mcpherson, E. G.] US Forest Serv, Pacific NW Res Stn, USDA, Davis, CA USA. RP Paine, TD (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM timothy.paine@ucr.edu NR 53 TC 0 Z9 0 U1 9 U2 37 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2497 EP 2504 DI 10.1093/jee/tov224 PG 8 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600001 PM 26470370 ER PT J AU Degrandi-Hoffman, G Chen, YP Dejong, EW Chambers, ML Hidalgo, G AF Degrandi-Hoffman, Gloria Chen, Yanping Dejong, Emily Watkins Chambers, Mona L. Hidalgo, Geoffrey TI Effects of Oral Exposure to Fungicides on Honey Bee Nutrition and Virus Levels SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE nutrition; deformed wing virus; immune function; boscalid; pyraclostrobin ID APIS-MELLIFERA L.; DEFORMED WING VIRUS; PESTICIDE EXPOSURE; PROTEIN DIETS; POLLEN; BEHAVIOR; NOSEMA; INFECTION; IPRODIONE; PATHOGEN AB Sublethal exposure to fungicides can affect honey bees ( Apis mellifera L.) in ways that resemble malnutrition. These include reduced brood rearing, queen loss, and increased pathogen levels. We examined the effects of oral exposure to the fungicides boscalid and pyraclostrobin on factors affecting colony nutrition and immune function including pollen consumption, protein digestion, hemolymph protein titers, and changes in virus levels. Because the fungicides are respiratory inhibitors, we also measured ATP concentrations in flight muscle. The effects were evaluated in 3- and 7- d- old worker bees at high fungicide concentrations in cage studies, and at field- relevant concentrations in colony studies. Though fungicide levels differed greatly between the cage and colony studies, similar effects were observed. Hemolymph protein concentrations were comparable between bees feeding on pollen with and without added fungicides. However, in both cage and colony studies, bees consumed less pollen containing fungicides and digested less of the protein. Bees fed fungicide- treated pollen also had lower ATP concentrations and higher virus titers. The combination of effects we detected could produce symptoms that are similar to those from poor nutrition and weaken colonies making them more vulnerable to loss from additional stressors such as parasites and pathogens. C1 [Degrandi-Hoffman, Gloria; Dejong, Emily Watkins; Chambers, Mona L.; Hidalgo, Geoffrey] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Chen, Yanping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. RP Degrandi-Hoffman, G (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. EM gloria.hoffman@ars.usda.gov NR 49 TC 1 Z9 1 U1 7 U2 34 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2518 EP 2528 DI 10.1093/jee/tov251 PG 11 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600004 PM 26318004 ER PT J AU Mustafa, T Alvarez, JM Munyaneza, JE AF Mustafa, T. Alvarez, J. M. Munyaneza, J. E. TI Effect of Cyantraniliprole on Probing Behavior of the Potato Psyllid (Hemiptera: Triozidae) as Measured by the Electrical Penetration Graph Technique SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bactericera cockerelli; Liberibacter; zebra chip; cyantraniliprole; abamectin ID ANTHRANILIC DIAMIDE INSECTICIDE; ZEBRA CHIP DISEASE; BACTERICERA-COCKERELLI; FEEDING-BEHAVIOR; DETERMINE REDUCTION; CAPSICUM-ANNUUM; MYZUS-PERSICAE; ASIAN CITRUS; TRANSMISSION; APHID AB Zebra chip (ZC), an economically important disease of potato, is caused by 'Candidatus Liberibacter solanacearum' (Lso) transmitted by the potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae). Currently, using insecticides against potato psyllid is the only means to manage ZC. However, the ability of the potato psyllid to rapidly transmit Lso represents a substantial challenge in preventing the spread of ZC. Cyantraniliprole, a novel second-generation anthranilic diamide insecticide has been shown to deter insect feeding and reduce disease transmission. During this study, the effect of cyantraniliprole on potato psyllid probing behavior was assessed using electrical penetration graph technology and compared with abamectin, a commonly used insecticide to control potato psyllid. Results showed that both cyantraniliprole and abamectin significantly deterred probing behavior of the potato psyllid. Average duration of intercellular stylet penetration on cyantraniliprole- and abamectin-treated and untreated control plants was 2.36, 1.80, and 9.15 h, respectively. It took psyllids 1.82, 1.10, and 2.42 h to reach the xylem of cyantraniliprole- and abamectin-treated and untreated plants, respectively. Xylem sap ingestion duration averaged 0.53, 0.57, and 3.66 h on cyantraniliprole- and abamectin-treated and untreated controls, respectively. None of the psyllids exposed to insecticide-treated plants reached the phloem tissue, except one that bypassed the xylem. The insects completely ceased probing after 4.44 and 3.64 h on cyantraniliprole- and abamectin-treated plants, respectively, in contrast with those on untreated plants that probed throughout the entire 24-h experiment duration. These results indicate that cyantraniliprole is as effective as abamectin in deterring potato psyllid feeding and could significantly reduce transmission of Lso and the spread of ZC. C1 [Mustafa, T.; Munyaneza, J. E.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Mustafa, T.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Alvarez, J. M.] Stine Haskell Res Ctr, DuPont Crop Protect, Newark, DE 19711 USA. RP Munyaneza, JE (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Joseph.Munyaneza@ars.usda.gov FU DuPont Crop Protection FX We thank Millie Heidt and Francisco de la Rosa for their technical assistance. We are also grateful to W. Rodney Cooper for his help with statistical analysis. Financial support for this research was partially provided by DuPont Crop Protection. NR 36 TC 4 Z9 4 U1 2 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2529 EP 2535 DI 10.1093/jee/tov259 PG 7 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600005 PM 26340224 ER PT J AU Sampson, BJ Rinehart, TA Kirker, GT Stringer, SJ Werle, CT AF Sampson, B. J. Rinehart, T. A. Kirker, G. T. Stringer, S. J. Werle, C. T. TI Phenotypic Variation in Fitness Traits of a Managed Solitary Bee, Osmia ribifloris (Hymenoptera: Megachilidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE mtDNA; conservation; usurpation; captivity; pollination ID LOCAL ADAPTATION; OUTBREEDING DEPRESSION; INBREEDING DEPRESSION; ROTUNDATA HYMENOPTERA; ECOLOGICAL GENETICS; NESTING SUCCESS; LEAFCUTTER BEE; SEX-RATIOS; MASON BEE; BODY-SIZE AB We investigated fitness in natural populations of a managed solitary bee Osmia ribifloris Cockerell (Hymenoptera: Megachilidae) from sites separated from 400 to 2,700 km. Parental wild bees originated in central Texas (TX), central-northern Utah (UT), and central California (CA). They were then intercrossed and raised inside a mesh enclosure in southern Mississippi (MS). Females from all possible mated pairs of O. ribifloris produced F-1 broods with 30-40% female cocoons and outcrossed progeny were 30% heavier. Mitochondrial (COI) genomes of the four populations revealed three distinct clades, a TX-CA clade, a UT clade, and an MS clade, the latter (MS) representing captive progeny of CA and UT bees. Although classified as separate subspecies, TX and CA populations from 30A degrees N to 38A degrees N latitude shared 98% similarity in COI genomes and the greatest brood biomass per nest straw (600- to 700-mg brood). Thus, TX and CA bees show greater adaptation for southern U.S. sites. In contrast, UT-sourced bees were more distantly related to TX and CA bees and also produced similar to 50% fewer brood. These results, taken together, confirm that adult O. ribifloris from all trap-nest sites are genetically compatible, but some phenotypic variation exists that could affect this species performance as a commercial blueberry pollinator. Males, their sperm, or perhaps a substance in their sperm helped stabilize our captive bee population by promoting legitimate nesting over nest usurpation. Otherwise, without insemination, 50% fewer females nested (they nested 14 d late) and 20% usurped nests, killing 33-67% of brood in affected nests. C1 [Sampson, B. J.; Rinehart, T. A.; Stringer, S. J.; Werle, C. T.] USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Kirker, G. T.] USDA FS Forest Prod Lab, Madison, WI 53726 USA. RP Sampson, BJ (reprint author), USDA ARS, Thad Cochran Southern Hort Lab, 810 Hwy 26 West, Poplarville, MS 39470 USA. EM blair.sampson@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service CRIS [6404-21430-001-00D] FX We are grateful to Jim Cane and Jack Neff for providing bees and Ray Langlois for field assistance. We also thank anonymous reviewers for greatly improving the manuscript. This research was funded by the United States Department of Agriculture, Agricultural Research Service CRIS Project number 6404-21430-001-00D. NR 60 TC 0 Z9 0 U1 4 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2589 EP 2598 DI 10.1093/jee/tov233 PG 10 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600012 PM 26470379 ER PT J AU Zhu, YC Adamczyk, J Rinderer, T Yao, JX Danka, R Luttrell, R Gore, J AF Zhu, Yu Cheng Adamczyk, John Rinderer, Thomas Yao, Jianxiu Danka, Robert Luttrell, Randall Gore, Jeff TI Spray Toxicity and Risk Potential of 42 Commonly Used Formulations of Row Crop Pesticides to Adult Honey Bees (Hymenoptera: Apidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE insecticide; spray; toxicity; honey bee; risk assessment ID TARNISHED PLANT BUG; SEED-TREATED CANOLA; APIS-MELLIFERA; HEMIPTERA; MIRIDAE; COTTON; REPLICATION; EXPOSURE; HEALTH AB To combat an increasing abundance of sucking insect pests, > 40 pesticides are currently recommended and frequently used as foliar sprays on row crops, especially cotton. Foraging honey bees may be killed when they are directly exposed to foliar sprays, or they may take contaminated pollen back to hives that maybe toxic to other adult bees and larvae. To assess acute toxicity against the honey bee, we used a modified spray tower to simulate field spray conditions to include direct whole-body exposure, inhalation, and continuing tarsal contact and oral licking after a field spray. A total of 42 formulated pesticides, including one herbicide and one fungicide, were assayed for acute spray toxicity to 4-6-d-old workers. Results showed significantly variable toxicities among pesticides, with LC(50)s ranging from 25 to thousands of mg/liter. Further risk assessment using the field application concentration to LC1 or LC99 ratios revealed the risk potential of the 42 pesticides. Three pesticides killed less than 1% of the worker bees, including the herbicide, a miticide, and a neonicotinoid. Twenty-six insecticides killed more than 99% of the bees, including commonly used organophosphates and neonicotinoids. The remainder of the 13 chemicals killed from 1-99% of the bees at field application rates. This study reveals a realistic acute toxicity of 42 commonly used foliar pesticides. The information is valuable for guiding insecticide selection to minimize direct killing of foraging honey bees, while maintaining effective control of field crop pests. C1 [Zhu, Yu Cheng; Yao, Jianxiu; Luttrell, Randall] USDA ARS, Stoneville, MS 38776 USA. [Adamczyk, John] USDA ARS, Poplarville, MS 39470 USA. [Rinderer, Thomas; Danka, Robert] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. [Gore, Jeff] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. RP Zhu, YC (reprint author), USDA ARS, Stoneville, MS 38776 USA. EM yc.zhu@ars.usda.gov NR 36 TC 2 Z9 2 U1 24 U2 57 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2640 EP 2647 DI 10.1093/jee/tov269 PG 8 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600017 PM 26352753 ER PT J AU Bredeson, MM Lundgren, JG AF Bredeson, Michael M. Lundgren, Jonathan G. TI Thiamethoxam Seed Treatments Have No Impact on Pest Numbers or Yield in Cultivated Sunflowers SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Helianthus annuus; herbivore; neonicotinoid; metabolite; clothianidin ID NEONICOTINOID INSECTICIDES; NATURAL ENEMIES; APIS-MELLIFERA; PLANTING DATE; SOYBEAN APHID; MANAGEMENT; CLOTHIANIDIN; COLEOPTERA; TOXICITY AB The use of neonicotinoid seed treatments is a nearly ubiquitous practice in sunflower (Helianthus annuus) pest management. Sunflowers have a speciose pest complex, but also harbor a diverse and abundant community of beneficial, nontarget organisms which may be negatively affected by pest management practices. Here, we investigate how the foliar and subterranean arthropod pest communities in sunflower fields were affected by a thiamethoxam seed treatment over three site years (two years on one farm, and another year at an additional field in the second year). Thiamethoxam and its metabolite clothianidin in leaf tissue were quantified throughout the growing season, and yield differences between treatments were measured. Across site years, foliar herbivores and key pests of sunflowers were unaffected by the seed treatment. Likewise, subterranean herbivores were unaffected. Thiamethoxam was measurable in leaf tissue through the R1 plant stage, while its metabolite clothianidin was detected throughout flowering (R6). No difference in sunflower yield was observed between treatments across site years. This research suggests that neonicotinoid seed treatments in sunflowers do not always provide economic benefits to farmers in the form of pest reductions or yield improvements. Future research should focus on sunflower integrated pest management strategies that limit nontarget effects of agrochemicals, while providing greater economic returns to farmers. C1 [Bredeson, Michael M.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. EM jgl.entomology@gmail.com NR 39 TC 1 Z9 1 U1 6 U2 22 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2665 EP 2671 DI 10.1093/jee/tov249 PG 7 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600020 PM 26340223 ER PT J AU Demkovich, M Dana, CE Siegel, JP Berenbaum, MR AF Demkovich, Mark Dana, Catherine E. Siegel, Joel P. Berenbaum, May R. TI Effect of Piperonyl Butoxide on the Toxicity of Four Classes of Insecticides to Navel Orangeworm (Amyelois transitella) (Lepidoptera: Pyralidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Amyelois transitella; cytochrome P450 monooxygenase; detoxification; insecticide; piperonyl butoxide ID LEPTINOTARSA-DECEMLINEATA SAY; PYRETHROID INSECTICIDES; RESISTANCE MECHANISMS; SUSCEPTIBILITY LEVEL; HUBNER LEPIDOPTERA; ALMOND; NOCTUIDAE; SYNERGISM; ORGANOPHOSPHATE; TORTRICIDAE AB Amyelois transitella (Walker) (Lepidoptera: Pyralidae), the navel orangeworm, is a highly polyphagous economic pest of almond, pistachio, and walnut crops in California. Increasing demand for these crops and their rising economic value has resulted in substantial increases of insecticide applications to reduce damage to acceptable levels. The effects of piperonyl butoxide (PBO), a methylenedioxyphenyl compound that can act as a synergist by inhibiting cytochrome P450-mediated detoxification on insecticide metabolism by A. transitella, were examined in a series of feeding bioassays with first-instar A. transitella larvae from a laboratory strain. PBO, however, can have a variety of effects on metabolism, including inhibition of glutathione-S-transferases and esterases and induction of P450s. In our study, PBO synergized the toxicity of acetamiprid, lambda-cyhalothrin, and spinosad, suggesting possible involvement of P450s in their detoxification. In contrast, PBO interacted antagonistically with the organophosphate insecticide chlorpyrifos, reducing its toxicity, an effect consistent with inhibition of P450-mediated bioactivation of this pesticide. The toxicity of the anthranilic diamide insecticide chlorantraniliprole was not altered by PBO, suggestive of little or no involvement of P450-mediated metabolism in its detoxification. Because a population of navel orangeworm in Kern County, CA, has already acquired resistance to the pyrethroid insecticide bifenthrin through enhanced P450 activity, determining the effect of adding a synergist such as PBO on detoxification of all insecticide classes registered for use in navel orangeworm management can help to develop rotation practices that may delay resistance acquisition or to implement alternative management practices where resistance is likely to evolve. C1 [Demkovich, Mark; Dana, Catherine E.; Berenbaum, May R.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Siegel, Joel P.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Demkovich, M (reprint author), Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. EM mdemkov3@illinois.edu FU Almond Board of California; California Pistachio Research Board FX We thank the Almond Board of California and the California Pistachio Research Board for funding this research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or the University of Illinois. USDA is an equal opportunity provider and employer. NR 39 TC 2 Z9 2 U1 6 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2753 EP 2760 DI 10.1093/jee/tov237 PG 8 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600030 PM 26470383 ER PT J AU Tilley, DR Casada, ME Langemeier, MR Subramanyam, B Arthur, FH AF Tilley, Dennis R. Casada, Mark E. Langemeier, Michael R. Subramanyam, Bhadriraju Arthur, Frank H. TI Economic Analysis for Commingling Effects of Insect Activity in the Elevator Boot Area SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE partial budget; stochastic dominance; risk; pest management ID GRAIN; WHEAT AB Boot areas in commercial grain elevators and feed mills contribute to commingling of insects with grain that moves through the elevator leg. A partial budget and stochastic dominance model were developed to improve pest management decision-making and risk analysis assessment from commingling effects of insect activity in the boot area. Modified pilot-scale bucket elevator legs, containing residual wheat or corn, were infested with varying insect pest densities prior to clean grain transfers. Appropriate grain discounts were applied to grain samples obtained from clean grain transfers over either: 1) insect-free and untreated boots, 2) infested and untreated boots, or 3) infested and chemical-treated (beta-cyfluthrin) boots. The insect-free boots simulated performing clean-out of the boot area. Partial budget analysis and stochastic dominance modeling indicated that boot sanitation (cleanout) about every 30 d, avoiding costly grain discounts from insect commingling, is the preferred choice. Although chemical spray treatments of the empty boot may reduce insect populations of some boot residual grains, boot cleanout always had lower and usually zero insect pest populations in the boot residual grain, providing higher facility operational net income without the use of chemicals. C1 [Tilley, Dennis R.; Casada, Mark E.; Arthur, Frank H.] USDA ARS, CGAHR, Manhattan, KS 66502 USA. [Casada, Mark E.] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. [Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. EM mark.casada@ars.usda.gov FU Andersons Research Grant Program; USDA Agricultural Research Service (CRIS) [5430-43440-007]; Kansas Agricultural Experiment Station [15-225-J] FX The research was supported by the Andersons Research Grant Program, by the USDA Agricultural Research Service (CRIS No. 5430-43440-007), and by the Kansas Agricultural Experiment Station (contribution number 15-225-J). NR 22 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD DEC PY 2015 VL 108 IS 6 BP 2800 EP 2807 DI 10.1093/jee/tov222 PG 8 WC Entomology SC Entomology GA CZ6AD UT WOS:000367182600035 PM 26470368 ER PT J AU Marcarelli, AM Huckins, CJ Eggert, SL AF Marcarelli, Amy M. Huckins, Casey J. Eggert, Susan L. TI Sand aggradation alters biofilm standing crop and metabolism in a low-gradient Lake Superior tributary SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Biofilm; Fine sediment; Lake Superior; Metabolism; Stream habitat ID COASTER BROOK TROUT; HEADWATER STREAMS; FINE SEDIMENT; ORGANIC-MATTER; LAND-USE; INVERTEBRATE COLONIZATION; BENTHIC INVERTEBRATES; ECOSYSTEM METABOLISM; PERIPHYTON BIOMASS; WATER-QUALITY AB Sediment deposition changes the physical characteristics of river beds, and may alter the production and/or processing of allochthonous and autochthonous organic matter, with potential consequences for stream food webs. We conducted a comparative study of biofilm standing crop and metabolism in the Salmon Trout River, a tributary of Lake Superior where watershed disturbances have led to 3-fold increases in streambed fine sediments, predominately sand, in the past decade. We compared biofilm standing crop and metabolism rates using light-dark chambers in reaches where substrate consisted of predominately exposed rock or sand substrates. Biofilm chlorophyll a was 4.2-fold greater on rock substrates, but ash-free dry mass was 8-fold greater on sand substrates. Rates of gross primary production were 2 to 25-fold greater on rock versus sand substrates, and differences persisted whether rates were scaled for area or biofilm standing crop. In contrast, rates of respiration were similar on rock and sand substrates when scaled per unit area but 7-13 times lower on sand when scaled for biofilm standing crop. Together, these results suggest that sand aggradation in this river alters organic matter processing during the summer from net production to net consumption and storage of organic matter. Sand aggradation may alter the availability and processing of both allochthonous and autochthonous food resources in this forested river, with potential far-reaching impacts on the food web. (C) 2015 International Association for Great Lakes Research. Published by Elsevier B.V. All rights reserved. C1 [Marcarelli, Amy M.; Huckins, Casey J.] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA. [Eggert, Susan L.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MI 55744 USA. RP Marcarelli, AM (reprint author), Michigan Technol Univ, Dept Biol Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. EM ammarcar@mtu.edu OI Marcarelli, Amy/0000-0002-4175-9211 FU Huron Mountain Wildlife Foundation; Michigan Tech's Research Excellence Fund-Research Seed program; USDA Forest Service Northern Research Station FX This research was supported by funding from the Huron Mountain Wildlife Foundation, Michigan Tech's Research Excellence Fund-Research Seed program, and the USDA Forest Service Northern Research Station. Jamey Anderson, Jonathan Ebel, Kyle Forgette, Michelle Kelly, Jeffery Kiiskila, Tony Matthys, Phillip Mercier, Mike Nagel, Stephani Nelson, Shane Rasley, Liz Turoz, Ryan Van Goethem, Tim Veverica, and Travis Wuori all provided valuable assistance in the field and in the laboratory. Jamey Anderson prepared Fig. 2. This manuscript benefitted from critical reviews by Ashley Coble and Tony Matthys. This is Contribution No. 25 of the Great Lakes Research Center at Michigan Tech. NR 69 TC 0 Z9 0 U1 7 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD DEC PY 2015 VL 41 IS 4 BP 1052 EP 1059 DI 10.1016/j.jglr.2015.09.004 PG 8 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CZ8OU UT WOS:000367359800010 ER PT J AU Becker, C Lord, SR Studenski, SA Warden, SJ Fielding, RA Recknor, CP Hochberg, MC Ferrari, SL Blain, H Binder, EF Rolland, Y Poiraudeau, S Benson, CT Myers, SL Hu, LJ Ahmad, QI Pacuch, KR Gomez, EV Benichou, O AF Becker, Clemens Lord, Stephen R. Studenski, Stephanie A. Warden, Stuart J. Fielding, Roger A. Recknor, Christopher P. Hochberg, Marc C. Ferrari, Serge L. Blain, Hubert Binder, Ellen F. Rolland, Yves Poiraudeau, Serge Benson, Charles T. Myers, Stephen L. Hu, Leijun Ahmad, Qasim I. Pacuch, Kelli R. Gomez, Elisa V. Benichou, Olivier CA STEADY Grp TI Myostatin antibody (LY2495655) in older weak fallers: a proof-of-concept, randomised, phase 2 trial SO LANCET DIABETES & ENDOCRINOLOGY LA English DT Article ID 6-MINUTE WALK TEST; LEAN BODY-MASS; PHYSICAL FUNCTION; MUSCLE STRENGTH; PERFORMANCE; ADULTS; EXERCISE; MOBILITY; PEOPLE; POWER AB Background Myostatin inhibits skeletal muscle growth. The humanised monoclonal antibody LY2495655 (LY) binds and neutralises myostatin. We aimed to test whether LY increases appendicular lean body mass (aLBM) and improves physical performance in older individuals who have had recent falls and low muscle strength and power. Methods In this proof-of-concept, randomised, placebo-controlled, double-blind, parallel, multicentre, phase 2 study, we recruited patients aged 75 years or older who had fallen in the past year from 21 investigator sites across Argentina, Australia, France, Germany, Sweden, and the USA. Eligible patients had low performance on hand grip strength and chair rise tests, tested with the procedure described by Guralnik and colleagues. Participants were stratified by country, age, hand grip strength, and performance on the chair rise test, and were randomly assigned (1: 1) by a computer-generated random sequence to receive subcutaneous injections of placebo or 315 mg LY at weeks 0 (randomisation visit), 4, 8, 12, 16, and 20, followed by 16 weeks observation. The primary outcome was change in aLBM from baseline to 24 weeks. We measured physical performance as secondary outcomes (four-step stair climbing time, usual gait speed, and time to rise five times from a chair without arms, or with arms for participants unable to do it without arms) and exploratory outcomes (12-step stair climbing test, 6-min walking distance, fast gait speed, hand grip strength, and isometric leg extension strength). Efficacy analyses included all randomly assigned patients who received at least one dose and had a baseline and at least one subsequent measure. The primary analysis and all other tests of treatment effect (except physical performance tests) were done at a two-sided alpha level of 0.05. Tests of treatment effect on physical performance tests were done at a pre-specified two-sided alpha level of 0.1. This trial is registered with ClinicalTrials.gov, number NCT01604408. Findings Between June 19, 2012, and Dec 12, 2013, we screened 365 patients. 99 were randomly assigned to receive placebo and 102 to receive LY. Treatment was completed in 85 (86%) of patients given placebo and in 82 (80%) given LY. At 24 weeks, the least-squares mean change in aLBM was -0.123 kg (95% CI -0.287 to 0.040) in the placebo group and 0.303 kg (0.135 to 0.470) in the LY group, a difference of 0.43 kg (95% CI 0.192 to 0.660; p<0.0001). Stair climbing time (four-step and 12-step tests), chair rise with arms, and fast gait speed improved significantly from baseline to week 24 with differences between LY and placebo of respectively -0.46 s (p=0.093), -1.28 s (p=0.011), -4.15 s (p=0.054), and 0.05 m/s (p=0.088). No effect was detected for other performance-based measures. Injection site reactions were recorded in nine (9%) patients given placebo and in 31 (30%) patients given LY (p<0.0001), and were generally mild, and led to treatment discontinuation in two patients given LY. Interpretation Our findings show LY treatment increases lean mass and might improve functional measures of muscle power. Although additional studies are needed to confirm these results, our data suggest LY should be tested for its potential ability to reduce the risk of falls or physical dependency in older weak fallers. C1 [Becker, Clemens] Robert Bosch Krankenhaus, Stuttgart, Germany. [Lord, Stephen R.] UNSW, NeuRA, Sydney, NSW, Australia. [Studenski, Stephanie A.] Univ Pittsburgh, Pittsburgh, PA USA. [Warden, Stuart J.] Indiana Univ, Sch Hlth & Rehabil Sci, Indianapolis, IN 46204 USA. [Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Recknor, Christopher P.] United Osteoporosis Ctr, Gainesville, GA USA. [Hochberg, Marc C.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. [Ferrari, Serge L.] Univ Hosp Geneva, Geneva, Switzerland. [Blain, Hubert] Univ Montpellier, Montpellier Univ Hosp, MACVIA LR, Dept Internal Med & Geriatr,M2H, F-34059 Montpellier, France. [Binder, Ellen F.] Washington Univ, Sch Med, St Louis, MO USA. [Rolland, Yves] Ctr Hosp Univ Toulouse, Gerontopole Toulouse, Toulouse, France. [Rolland, Yves] INSERM 1027, Toulouse, France. [Poiraudeau, Serge] Cochin Hosp, Dept Phys Rehabil Musculoskeletal & Spinal Disord, Paris, France. [Poiraudeau, Serge] Paris Descartes Univ, Paris, France. [Poiraudeau, Serge] INSERM U1153, Paris, France. [Benson, Charles T.; Myers, Stephen L.; Hu, Leijun; Ahmad, Qasim I.; Pacuch, Kelli R.; Gomez, Elisa V.] Eli Lilly & Co, Indianapolis, IN 46285 USA. [Benichou, Olivier] Eli Lilly & Co, Neuilly, France. RP Benichou, O (reprint author), Eli Lilly & Co, Neuilly, France. EM benichou_olivier@lilly.com RI Buehring, Bjoern/L-5581-2013; POIRAUDEAU, SERGE/P-5301-2016; OI Buehring, Bjoern/0000-0003-3841-624X; Warden, Stuart/0000-0002-6415-4936 FU Eli Lilly and Company FX This study was funded by Eli Lilly and Company. We thank all the patients who participated in this research; all the trial investigators who are listed in the appendix (p 1); Barbara M Coffey for her diligent and effective medical writing support; and Dylan Kirn and Kieran Reid for their help in training and certification of study personnel for physical performance assessment. SAS' authorship relates to work done before her federal employment, and the views expressed in the publication do not necessarily represent the views of the National Institutes of Health or the USA. NR 40 TC 19 Z9 19 U1 7 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2213-8587 J9 LANCET DIABETES ENDO JI Lancet Diabetes Endocrinol. PD DEC PY 2015 VL 3 IS 12 BP 948 EP 957 DI 10.1016/S2213-8587(15)00298-3 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CZ8ZM UT WOS:000367387600023 PM 26516121 ER PT J AU Pigeot, I Baranowski, T De Henauw, S AF Pigeot, I. Baranowski, T. De Henauw, S. CA IDEFICS Intervention Study Grp IDEFICS Consortium TI The IDEFICS intervention trial to prevent childhood obesity: design and study methods SO OBESITY REVIEWS LA English DT Article DE Childhood obesity; Europe; evaluation; primary prevention ID COMMUNITY-BASED INTERVENTION; FOCUS GROUPS; NUTRITION; CHILDREN; DEVELOP; PARENTS; PROGRAM; MODEL AB IntroductionOne of the major research dimensions of the Identification and prevention of Dietary- and lifestyle-induced health EFfects In Children and infantS (IDEFICS) study involved the development, implementation and evaluation of a setting-based community-oriented intervention programme for primary prevention of childhood obesity. In this supplement of Obesity Reviews, a compilation of key results of the IDEFICS intervention is packaged in a series of complementary papers. ObjectiveThis paper describes the overall design and methods of the IDEFICS intervention in order to facilitate a comprehensive reading of the supplement. In addition, some best practice' examples are described. ResultsThe IDEFICS intervention trial was conducted to assess whether the IDEFICS intervention prevented obesity in young children aged 2 to 9.9years. The study was a non-randomized, quasi-experimental trial with one intervention matched to one control region in each of eight participating countries. The intervention was designed following the intervention mapping framework, using a socio-ecological theoretical approach. The intervention was designed to address several key obesity-related behaviours in children, parents, schools and community actors; the primary outcome was the prevalence of overweight/obesity according to the IOTF criteria based on body mass index. The aim was to achieve a reduction of overweight/obesity prevalence in the intervention regions. The intervention was delivered in school and community settings over a 2-year period. Data were collected in the intervention and control cohort regions at baseline and 2 years later. ConclusionThis paper offers an introductory framework for a comprehensive reading of this supplement on IDEFICS intervention key results. C1 [Pigeot, I.] Leibniz Inst Prevent Res & Epidemiol BIPS, Bremen, Germany. [Pigeot, I.] Univ Bremen, Fac Math & Comp Sci, Inst Stat, D-28359 Bremen, Germany. [Baranowski, T.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [De Henauw, S.] Univ Ghent, Fac Med & Hlth Sci, Dept Publ Hlth, B-9000 Ghent, Belgium. RP De Henauw, S (reprint author), Univ Ghent, Fac Med & Hlth Sci, Dept Publ Hlth, B-9000 Ghent, Belgium. EM Stefaan.DeHenauw@UGent.be OI Baranowski, Tom/0000-0002-0653-2222; Fernandez-Alvira, Juan Miguel/0000-0002-4145-5103; Ahrens, Wolfgang/0000-0003-3777-570X FU European Community [016181]; Federal Centre for Health Education, Germany FX This work was done as part of the IDEFICS study (www.idefics.eu). We gratefully acknowledge the financial support of the European Community within the Sixth RTD Framework Programme Contract No. 016181 (FOOD). We are grateful for the support by school boards, headmasters and communities. The authors wish to thank the IDEFICS children and their parents for participating in this extensive examination. The authors are also grateful to the Federal Centre for Health Education, Germany, that financially supported the production of this supplement. NR 38 TC 17 Z9 17 U1 4 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7881 EI 1467-789X J9 OBES REV JI Obes. Rev. PD DEC PY 2015 VL 16 SU 2 SI SI BP 4 EP 15 DI 10.1111/obr.12345 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CZ8NM UT WOS:000367356400003 PM 26707012 ER PT J AU Baranowski, T Lytle, L AF Baranowski, T. Lytle, L. TI Should the IDEFICS outcomes have been expected? SO OBESITY REVIEWS LA English DT Article DE Obesity prevention; children; mediating variables ID CHILDHOOD OBESITY PREVENTION; SCHOOL-BASED INTERVENTION; COMMUNITY-BASED INTERVENTION; BODY-MASS INDEX; PHYSICAL-ACTIVITY; SLEEP DURATION; ENERGY-BALANCE; SEDENTARY BEHAVIOR; EUROPEAN CHILDREN; FAMILY DINNER AB The Identification and prevention of Dietary- and lifestyle-induced health EFfects In Children and infantS (IDEFICS) study evaluated with a large sample a comprehensive carefully planned obesity prevention intervention targeting multiple levels of influence that were culturally adapted to the situations in eight European countries. Despite the great effort and attention to detail, the IDEFICS study did not achieve its targeted adiposity or behaviour change objectives. Should we be surprised that the IDEFICS trial did not have its intended effects? We think not, and would have been surprised if it did. Recent research has revealed the lack of consistent meaningful relationships between several apparently obesogenic behaviours and adiposity, weak or no relationships among behavioural change procedures, mediating variables and targeted behaviours and inadequate attention to moderating effects. Future obesity prevention interventions would benefit from a more thorough understanding of the complex relationships that have been hypothesized and the interrelationships with biological factors. While systems modelling has been proposed as providing the solution, important less complex identification of new constructs, new relationships and community interventions are still needed, both to find innovative solutions and to provide input into the systems models. We should question results from cross-sectional studies and be satisfied only with longitudinal or experimental tests of relationships. C1 [Baranowski, T.] Baylor Univ, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr,Baylor Coll Med, Houston, TX 77030 USA. [Lytle, L.] Univ N Carolina, Dept Hlth Behav, Sch Global Publ Hlth, Chapel Hill, NC USA. RP Baranowski, T (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU USDA/ARS [58-6250-0-008]; Federal Centre for Health Education, Germany FX This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA, and had been funded in part with federal funds from the USDA/ARS under cooperative agreement no. 58-6250-0-008. The authors are also grateful to the Federal Centre for Health Education, Germany, which financially supported the production of this supplement. NR 140 TC 7 Z9 7 U1 4 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7881 EI 1467-789X J9 OBES REV JI Obes. Rev. PD DEC PY 2015 VL 16 SU 2 SI SI BP 162 EP 172 DI 10.1111/obr.12359 PG 11 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CZ8NM UT WOS:000367356400016 PM 26707025 ER PT J AU Mroz, T Havey, MJ Bartoszewski, G AF Mroz, Tomasz L. Havey, Michael J. Bartoszewski, Grzegorz TI Cucumber Possesses a Single Terminal Alternative Oxidase Gene That is Upregulated by Cold Stress and in the Mosaic (MSC) Mitochondrial Mutants SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Alternative oxidase; Cucumis sativus; Gene structure; Mitochondrial mutants ID HIGHER-PLANT MITOCHONDRIA; NAC TRANSCRIPTION FACTOR; RETROGRADE REGULATION; ARABIDOPSIS-THALIANA; GENOME REARRANGEMENT; OXIDATIVE STRESS; BIOTIC STRESS; EXPRESSION; SATIVUS; PROTEIN AB Alternative oxidase (AOX) is a mitochondrial terminal oxidase which is responsible for an alternative route of electron transport in the respiratory chain. This nuclear-encoded enzyme is involved in a major path of survival under adverse conditions by transfer of electrons from ubiquinol instead of the main cytochrome pathway. AOX protects against unexpected inhibition of the cytochrome c oxidase pathway and plays an important role in stress tolerance. Two AOX subfamilies (AOX1 and AOX2) exist in higher plants and are usually encoded by small gene families. In this study, genome-wide searches and cloning were completed to identify and characterize AOX genes in cucumber (Cucumis sativus L.). Our results revealed that cucumber possesses no AOX1 gene(s) and only a single AOX2 gene located on chromosome 4. Expression studies showed that AOX2 in wild-type cucumber is constitutively expressed at low levels and is upregulated by cold stress. AOX2 transcripts and protein were detected in leaves and flowers of wild-type plants, with higher levels in the three independently derived mosaic (MSC) mitochondrial mutants. Because cucumber possesses a single AOX gene and its expression increases under cold stress and in the MSC mutants, this plant is a unique and intriguing model to study AOX expression and regulation particularly in the context of mitochondria-to-nucleus retrograde signaling. C1 [Mroz, Tomasz L.; Bartoszewski, Grzegorz] Warsaw Univ Life Sci, Dept Plant Genet Breeding & Biotechnol, Fac Hort Biotechnol & Landscape Architecture, PL-02776 Warsaw, Poland. [Havey, Michael J.] Univ Wisconsin, ARS, USDA, Vegetable Crops Unit,Dept Hort, Madison, WI 53706 USA. RP Bartoszewski, G (reprint author), Warsaw Univ Life Sci, Dept Plant Genet Breeding & Biotechnol, Fac Hort Biotechnol & Landscape Architecture, Ul Nowoursynowska 159, PL-02776 Warsaw, Poland. EM grzegorz_bartoszewski@sggw.pl RI Bartoszewski, Grzegorz/M-8458-2016 OI Bartoszewski, Grzegorz/0000-0002-6197-770X FU Polish National Science Centre NCN project [N N310 107740] FX We thank Prof. Thomas Elthon (University of Nebraska) for providing us anti-AOX and anti-porin mouse monoclonal antibodies and Dr. Emilia J. Orzechowska (University of Warsaw, Poland) for consultation on the optimization of protein detection. This work was supported by Polish National Science Centre NCN project N N310 107740. The authors declare that there is no conflict of interest. NR 70 TC 1 Z9 1 U1 1 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD DEC PY 2015 VL 33 IS 6 BP 1893 EP 1906 DI 10.1007/s11105-015-0883-9 PG 14 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DA0CJ UT WOS:000367464000024 PM 26752808 ER PT J AU Sewell, F Ragan, I Marczylo, T Anderson, B Braun, A Casey, W Dennison, N Griffiths, D Guest, R Holmes, T van Huygevoort, T Indans, I Kenny, T Kojima, H Lee, K Prieto, P Smith, P Smedley, J Stokes, WS Wnorowski, G Horgan, G AF Sewell, Fiona Ragan, Ian Marczylo, Tim Anderson, Brian Braun, Anne Casey, Warren Dennison, Ngaire Griffiths, David Guest, Robert Holmes, Tom van Huygevoort, Ton Indans, Ian Kenny, Terry Kojima, Hajime Lee, Kyuhong Prieto, Pilar Smith, Paul Smedley, Jason Stokes, William S. Wnorowski, Gary Horgan, Graham TI A global initiative to refine acute inhalation studies through the use of 'evident toxicity' as an endpoint: Towards adoption of the fixed concentration procedure SO REGULATORY TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Acute inhalation studies; 3Rs; Evident toxicity; Fixed concentration procedure (FCP); Refinement; Regulatory toxicology; TG4303; TG436; TG433 ID GENDER-DIFFERENCES AB Acute inhalation studies are conducted in animals as part of chemical hazard identification and characterisation, including for classification and labelling purposes. Current accepted methods use death as an endpoint (OECD TG403 and TG436), whereas the fixed concentration procedure (FCP) (draft OECD TG433) uses fewer animals and replaces lethality as an endpoint with 'evident toxicity.' Evident toxicity is defined as clear signs of toxicity that predict exposure to the next highest concentration will cause severe toxicity or death in most animals. A global initiative including 20 organisations, led by the National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) has shared data on the clinical signs recorded during acute inhalation studies for 172 substances (primarily dusts or mists) with the aim of making evident toxicity more objective and transferable between laboratories. Pairs of studies (5 male or 5 female rats) with at least a two-fold change in concentration were analysed to determine if there are any signs at the lower dose that could have predicted severe toxicity or death at the higher concentration. The results show that signs such as body weight loss (>10% pre-dosing weight), irregular respiration, tremors and hypoactivity, seen at least once in at least one animal after the day of dosing are highly predictive (positive predictive value > 90%) of severe toxicity or death at the next highest concentration. The working group has used these data to propose changes to TG433 that incorporate a clear indication of the clinical signs that define evident toxicity. (C) 2015 The Authors. Published by Elsevier Inc. C1 [Prieto, Pilar] Commiss European Communities, Joint Res Ctr, Inst Hlth & Consumer Protect, EURL ECVAM,Syst Toxicol Unit, I-21020 Ispra, Italy. [Smith, Paul] Charles River Labs, Edinburgh, Midlothian, Scotland. [Smedley, Jason] Charles River Labs, Spencerville, OH 45887 USA. [Stokes, William S.] USDA, Anim & Plant Hlth Inspect Serv, Anim Care, Washington, DC USA. EM fiona.sewell@nc3rs.org.uk NR 5 TC 1 Z9 1 U1 1 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0273-2300 EI 1096-0295 J9 REGUL TOXICOL PHARM JI Regul. Toxicol. Pharmacol. PD DEC PY 2015 VL 73 IS 3 BP 770 EP 779 DI 10.1016/j.yrtph.2015.10.018 PG 10 WC Medicine, Legal; Pharmacology & Pharmacy; Toxicology SC Legal Medicine; Pharmacology & Pharmacy; Toxicology GA CZ7KX UT WOS:000367279400012 PM 26505531 ER PT J AU Raithore, S Bai, JH Plotto, A Manthey, J Irey, M Baldwin, E AF Raithore, Smita Bai, Jinhe Plotto, Anne Manthey, John Irey, Mike Baldwin, Elizabeth TI Electronic Tongue Response to Chemicals in Orange Juice that Change Concentration in Relation to Harvest Maturity and Citrus Greening or Huanglongbing (HLB) Disease SO SENSORS LA English DT Article DE sensor; quality; citrus; sugars; acids; limonoids; flavonoids; huanglongbing; harvest maturity; sensory ID FLAVOR QUALITY; LIMONOIDS; RECOGNITION; PERCEPTION; BEVERAGES; LIMONIN; TASTE AB In an earlier study, an electronic tongue system (e-tongue) has been used to differentiate between orange juice made from healthy fruit and from fruit affected by the citrus greening or Huanglongbing (HLB) disease. This study investigated the reaction of an e-tongue system to the main chemicals in orange juice that impact flavor and health benefits and are also impacted by HLB. Orange juice was spiked with sucrose (0.2-5.0 g/100 mL), citric acid (0.1%-3.0% g/100 mL) and potassium chloride (0.1-3.0 g/100 mL) as well as the secondary metabolites nomilin (1-30 mu g/mL), limonin (1-30 mu g/mL), limonin glucoside (30-200 mu g/mL), hesperidin (30-400 mu g/mL) and hesperetin (30-400 mu g/mL). Performance of Alpha MOS sensor sets #1 (pharmaceutical) and #5 (food) were compared for the same samples, with sensor set #1 generally giving better separation than sensor set #5 for sucrose, sensor set #5 giving better separation for nomilin and limonin, both sets being efficient at separating citric acid, potassium chloride, hesperitin and limonin glucoside, and neither set discriminating hesperidin efficiently. Orange juice made from fruit over the harvest season and from fruit harvested from healthy or HLB-affected trees were separated by harvest maturity, disease state and disease severity. C1 [Raithore, Smita; Bai, Jinhe; Plotto, Anne; Manthey, John; Baldwin, Elizabeth] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Irey, Mike] US Sugar Corp, Clewiston, FL 33400 USA. RP Baldwin, E (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM smitare@gmail.com; jinhe.bai@ars.usda.gov; anne.plotto@ars.usda.gov; john.manthey@ars.usda.gov; mirey@ussugar.com; liz.baldwin@ars.usda.gov NR 32 TC 1 Z9 2 U1 9 U2 20 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD DEC PY 2015 VL 15 IS 12 BP 30062 EP 30075 DI 10.3390/s151229787 PG 14 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA DA1EM UT WOS:000367539100022 PM 26633411 ER PT J AU Ferraz, TM Netto, AT Reis, FD Pecanha, AL De Sousa, EF Machado, JA Rodrigues, WP Glenn, DM Campostrini, E AF Ferraz, Tiago Massi Netto, Alena Torres Reis, Fabricio De Oliveira Pecanha, Anderson Lopes De Sousa, Elias Fernandes Machado Filho, Jose Altino Rodrigues, Weverton Pereira Glenn, David Michael Campostrini, Eliemar TI Relationships between sap-flow measurements, whole-canopy transpiration and reference evapotranspiration in field-grown papaya (Carica papaya L.) SO THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY LA English DT Article DE Gas exchange; Transpiration; Temperature; Water relations ID GAS-EXCHANGE SYSTEM; WATER RELATIONS; PLANT; YIELD; IRRIGATION; STRESS; TREES; LEAF; EQUATION; STEMS AB Whole-canopy gas exchange measurement in papaya can provide a scientific basis to optimize irrigation and fruit yield and quality. The objectives of this study were to: (1) verify the relationship between xylem sap flow measured by the heat coefficient method, and whole canopy transpiration in 'Gran Golden' papaya (Carica papaya L.), (2) examine the relationship between xylem sap flow determined from calculations based on forcing water flow through a stem section, and whole canopy transpiration, and (3) verify the relationship between transpiration measured in a plant chamber and calculated reference evapotranspiration (ET0). The experiment was conducted in a commercial planting at the Caliman Agricola SA farm, Sooretama, ES, Brazil in summer and winter. Whole-canopy gas exchange was measured in custom-built, flow-through whole-canopy Mylar (R) chambers. There was a correlation (r(2) = 0.65) between xylem sap flow measured with the heat coefficient (K) method and whole-canopy transpiration rate measured in the chambers. However, sap flow methodology could not convert this correlated response into an accurate estimate of papaya transpiration measured with whole canopy chambers. The sap flow methodologies were linearly correlated with whole canopy transpiration but each would require additional calibration to the crop. ET0 was correlated with whole canopy transpiration in winter but not in summer likely due to stomatal control of transpiration, as a result of the high leaf temperature and ET0 in summer. The concept of sap flow to measure transpiration has value in papaya production but supplementary studies will be necessary to improve the accuracy and estimation of the whole-canopy transpiration in the papaya plant. C1 [Ferraz, Tiago Massi; Reis, Fabricio De Oliveira] Univ Estadual Maranhao, CESI, Dept Biol & Chem, BR-65901480 Imperatriz, MA, Brazil. [Netto, Alena Torres; Pecanha, Anderson Lopes; Rodrigues, Weverton Pereira; Campostrini, Eliemar] State Univ North Fluminense, Agr Sci & Technol Ctr, LMGV, Plant Physiol Lab, BR-28013602 Campos Dos Goytacazes, RJ, Brazil. [De Sousa, Elias Fernandes] State Univ North Fluminense, Agr Sci & Technol Ctr, Irrigat Sect, BR-28013602 Campos Dos Goytacazes, RJ, Brazil. [Machado Filho, Jose Altino] Inst Pesquisa Assistencia Tecn & Extensa Rural Es, BR-29915140 Linhares, ES, Brazil. [Glenn, David Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Campostrini, E (reprint author), State Univ North Fluminense, Agr Sci & Technol Ctr, LMGV, Plant Physiol Lab, Ave Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil. EM ferraztm@gmail.com; campostenator@gmail.com RI Rodrigues, Weverton/H-4296-2016; OI Rodrigues, Weverton/0000-0002-9137-474X; Campostrini, Eliemar/0000-0002-1329-1084 FU Caliman Agricola S/A; Financiadora de Estudos e Projetos (FINEP, Brazil); Fundacao Carlos Chagas de Apoio a Pesquisa no Estado do Rio de Janeiro (FAPERJ, Brazil); National Council for Scientific and Technological Development (CNPq, Brazil); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil) FX We acknowledge the support of Caliman Agricola S/A, Financiadora de Estudos e Projetos (FINEP, Brazil), Fundacao Carlos Chagas de Apoio a Pesquisa no Estado do Rio de Janeiro (FAPERJ, Brazil), National Council for Scientific and Technological Development (CNPq, Brazil) and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES, Brazil). We are also thankful to Dynamax (Houston, Texas, USA) for providing the probes to measure the xylem sap flow. We are also thankful to Dr. Bruce Schaffer, Tropical Research & Education Center (TREC), University of Florida, USA for helpful grammar corrections. NR 49 TC 1 Z9 1 U1 2 U2 16 PU BRAZILIAN SOC PLANT PHYSIOLOGY PI CAMPINAS PA DEPT FISIOLOGIA VEGETAL - IB, UNICAMP, CP 6109, CAMPINAS, SP 00000, BRAZIL SN 2197-0025 J9 THEOR EXP PLANT PHYS JI Theor. Exp. Plant Physiol. PD DEC PY 2015 VL 27 IS 3-4 BP 251 EP 262 DI 10.1007/s40626-015-0049-z PG 12 WC Plant Sciences SC Plant Sciences GA CZ8XA UT WOS:000367381200008 ER PT J AU Shao, YP Nickling, W Bergametti, G Butler, H Chappell, A Findlater, P Gillies, J Ishizuka, M Klose, M Kok, JF Leys, J Lu, H Marticorena, B McTainsh, G McKenna-Neuman, C Okin, GS Strong, C Webb, N AF Shao, Yaping Nickling, William Bergametti, Gilles Butler, Harry Chappell, Adrian Findlater, Paul Gillies, John Ishizuka, Masahide Klose, Martina Kok, Jasper F. Leys, John Lu, Hua Marticorena, Beatrice McTainsh, Grant McKenna-Neuman, Cheryl Okin, Gregory S. Strong, Craig Webb, Nicholas TI A tribute to Michael R. Raupach for contributions to aeolian fluid dynamics SO AEOLIAN RESEARCH LA English DT Review DE Michael R. Raupach; Drag partition; Roughness; Dust emission; Threshold friction velocity; Carbon cycle ID ZERO-PLANE DISPLACEMENT; ATMOSPHERIC INERTIAL SUBLAYER; SOIL SPECTRAL REFLECTANCE; TURBULENT DUST EMISSION; WIND EROSION THRESHOLD; AERODYNAMIC ROUGHNESS; DRAG PARTITION; MINERAL DUST; SIZE DISTRIBUTIONS; SALTATION BOMBARDMENT AB Since the pioneering work of Bagnold in the 1940s, aeolian research has grown to become an integral part of earth-system science. Many individuals have contributed to this development, and Dr. Michael R. Raupach (1950-2015) has played a pivotal role. Raupach worked intensively on wind erosion problems for about a decade (1985-1995), during which time he applied his deep knowledge of turbulence to aeolian research problems and made profound contributions with far-reaching impact. The beauty of Raupach's work lies in his clear conceptual thinking and his ability to reduce complex problems to their bare essentials. The results of his work are fundamentally important and have many practical applications. In this review we reflect on Raupach's contribution to a number of important aspects of aeolian research, summarise developments since his inspirational work and place Raupach's efforts in the context of aeolian science. We also demonstrate how Raupach's work provided a foundation for new developments in aeolian research. In this tribute, we concentrate on five areas of research: (1) drag partition theory; (2) saltation roughness length; (3) saltation bombardment; (4) threshold friction velocity and (5) the carbon cycle. (C) 2015 Elsevier B.V. All rights reserved. C1 [Shao, Yaping; Klose, Martina] Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany. [Nickling, William] Univ Guelph, Dept Geog, Wind Eros Lab, Guelph, ON N1G 2W1, Canada. [Bergametti, Gilles; Marticorena, Beatrice] Univ Paris Diderot, Lab Interuniv Syst Atmospher, Paris, France. [Bergametti, Gilles; Marticorena, Beatrice] Univ Paris Est, Lab Interuniv Syst Atmospher, Creteil, France. [Butler, Harry] Univ So Queensland, Dept Math & Comp, Toowoomba, Qld 4350, Australia. [Chappell, Adrian] CSIRO Land & Water Natl Res Flagship, Canberra, ACT, Australia. [Findlater, Paul] WA Dept Agr & Food, Geraldton, Australia. [Gillies, John] Univ Nevada, Desert Res Inst, Div Atmospher Sci, Reno, NV 89506 USA. [Ishizuka, Masahide] Kagawa Univ, Fac Engn, Takamatsu, Kagawa 760, Japan. [Kok, Jasper F.] Univ Calif Los Angeles, Dept Atmospher & Ocean Sci, Los Angeles, CA USA. [Leys, John] NSW Off Environm & Heritage, Gunnedah, Australia. [Lu, Hua] British Antarctic Survey, Cambridge CB3 0ET, England. [McTainsh, Grant] Griffith Univ, Griffith Sch Environm, Nathan, Qld 4111, Australia. [McKenna-Neuman, Cheryl] Trent Univ, Dept Geog, Peterborough, ON K9J 7B8, Canada. [Okin, Gregory S.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Strong, Craig] Australian Natl Univ, Canberra, ACT, Australia. [Webb, Nicholas] USDA ARS Jornada Expt Range, Las Cruces, NM USA. RP Shao, YP (reprint author), Univ Cologne, Inst Geophys & Meteorol, Cologne, Germany. EM yshao@uni-koeln.de; nickling@uoguelph.ca; Gilles.Bergametti@lisa.u-pec.fr; butler@usq.edu.au; adrian.chappell@csiro.au; paul.findlater@agric.wa.gov.au; Jack.Gillies@dri.edu; ishizuka@eng.kagawa-u.ac.jp; mklose@uni-koeln.de; jfltok@ucla.edu; John.Leys@environment.nsw.gov.au; hlu@bas.ac.uk; Beatrice.Marticorena@lisa.u-pec.fr; g.mctainsh@griffith.edu.au; cmckneuman@trentu.ca; okin@ucla.edu; craig.strong@anu.edu.au; nwebb@nmsu.edu RI Chappell, Adrian/A-7058-2011; Shao, Yaping/G-3606-2013; Kok, Jasper/A-9698-2008; OI Chappell, Adrian/0000-0002-0694-7348; Shao, Yaping/0000-0002-2041-5479; Kok, Jasper/0000-0003-0464-8325; Klose, Martina/0000-0001-8190-3700 NR 135 TC 4 Z9 4 U1 7 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD DEC PY 2015 VL 19 BP 37 EP 54 DI 10.1016/j.aeolia.2015.09.004 PN A PG 18 WC Geography, Physical SC Physical Geography GA CZ3RY UT WOS:000367022900004 ER PT J AU Li, HL Tatarko, J Kucharski, M Dong, Z AF Li, Hongli Tatarko, John Kucharski, Matthew Dong, Zhi TI PM2.5 and PM10 emissions from agricultural soils by wind erosion SO AEOLIAN RESEARCH LA English DT Article DE Dust emission; PM2.5/PM10 ratio; Wind tunnel; Conventional tillage; No-till ID RESUSPENSION TEST CHAMBER; PREDICTION SYSTEM WEPS; DAILY MORTALITY; SEDIMENT TRANSPORT; COLUMBIA PLATEAU; FINE PARTICLES; AIR-POLLUTION; VISIBILITY; SUSPENSION; CALIFORNIA AB Soil tillage and wind erosion are two major emission sources of particulate matter less than 2.5 and 10 mu m in size (PM2.5 and PM10) from cultivated soils. Samples from fifteen cultivated soils from five states of the US, both crushed (<2.0 mm) and uncrushed (natural aggregation), were tested for PM2.5 and PM10 emissions in a wind tunnel, at 8, 10, and 13 m s(-1) wind velocities. In addition, 10 soils were paired as conventional vs. no-till. Results showed that: (1) PM2.5 and PM10 emissions of crushed and uncrushed samples increased with wind speed, with a more rapid and greater emissions trend for crushed samples; after three wind speeds, mean PM2.5 and PM10 emissions from crushed soils were 1.3-8.5 and 1.9-10 times that of uncrushed soils; (2) PM2.5/PM10 ratios of crushed and uncrushed samples were, respectively, 0.11-0.45 and 0.13-0.46, and the mean PM2.5/PM10 ratio of uncnshed samples was higher; (3) PM2.5 and PM10 emissions of tested soils showed a significant negative power function relationship with clay content and PM2.5 and PM10 fractions of the dispersed soil samples, whereas the sand content and <0.42-mm aggregate content of the samples showed a significant linear positive correlation with emissions; and (4) although not significant, no-till soils had consistently lower PM2.5 and PM10 emissions than paired conventional tilled soils for uncrushed samples. Published by Elsevier B.V. C1 [Li, Hongli; Dong, Zhi] Shandong Agr Univ, Coll Forestry, Tai An, Shandong, Peoples R China. [Tatarko, John] ARS, USDA, Agr Syst Res Unit, Ft Collins, CO USA. [Kucharski, Matthew] ARS, USDA, Agr Syst Res Unit, Manhattan, KS USA. RP Tatarko, J (reprint author), 2150 Ctr Ave,Bldg D,Suite 200, Ft Collins, CO 80526 USA. EM John.Tatarko@ars.usda.gov NR 46 TC 0 Z9 0 U1 10 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD DEC PY 2015 VL 19 SI SI BP 171 EP 182 DI 10.1016/j.aeolia.2015.02.003 PN B PG 12 WC Geography, Physical SC Physical Geography GA CZ3RZ UT WOS:000367023000004 ER PT J AU Gadde, U Rathinam, T Lillehoj, HS AF Gadde, U. Rathinam, T. Lillehoj, Hyun S. TI Passive immunization with hyperimmune egg-yolk IgY as prophylaxis and therapy for poultry diseases - A review SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Article DE hyperimmune IgY; egg yolk antibodies; enteric infections; poultry ID ENTEROTOXIGENIC ESCHERICHIA-COLI; INFECTIOUS BURSAL DISEASE; CHICKEN EGG; SALMONELLA-ENTERITIDIS; CAMPYLOBACTER-JEJUNI; ANTIBODY IGY; IMMUNOGLOBULIN-Y; IN-VITRO; EIMERIA-ACERVULINA; BROILER-CHICKENS AB Passive immunization with pathogen-specific egg yolk antibodies (IgY) is emerging as a potential alternative to antibiotics for the treatment and prevention of various human and animal diseases. Laying hens are an excellent source of high-quality polyclonal antibodies, which can be collected noninvasively from egg yolks. The use of IgY offers several advantages in that it is environmentally friendly, nontoxic, and reduces the numbers of animals required for antibody production. This paper reviews the use of IgY antibodies in the treatment and prevention of enteric pathogen infections in poultry. Brief descriptions of the production, structure, and properties of IgY are also presented. Some limitations of the technology and future perspectives are discussed. C1 [Gadde, U.; Lillehoj, Hyun S.] USDA ARS, Beltsville Agr Res Ctr, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Gadde, U.] USDA ARS, Beltsville Agr Res Ctr, ORISE Res Participat Program, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. RP Lillehoj, HS (reprint author), USDA ARS, Beltsville Agr Res Ctr, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. EM hyun.lillehoj@ars.usda.gov FU ARS-USDA CRIS in Animal Health (NP103) [8042-32000-097-00D] FX This project was supported by ARS-USDA CRIS in Animal Health (NP103) # 8042-32000-097-00D. NR 149 TC 0 Z9 0 U1 9 U2 20 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 EI 1475-2654 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD DEC PY 2015 VL 16 IS 2 BP 163 EP 176 DI 10.1017/S1466252315000195 PG 14 WC Veterinary Sciences SC Veterinary Sciences GA CZ5YI UT WOS:000367177900006 PM 26568433 ER PT J AU Yin, ZW Shi, FC Jiang, HM Roberts, DP Chen, SF Fan, BQ AF Yin, Zhongwei Shi, Fachao Jiang, Hongmei Roberts, Daniel P. Chen, Sanfeng Fan, Bingquan TI Phosphate solubilization and promotion of maize growth by Penicillium oxalicum P4 and Aspergillus niger P85 in a calcareous soil SO CANADIAN JOURNAL OF MICROBIOLOGY LA English DT Article DE Aspergillus niger; calcareous soil; Penicillium oxalicum; phosphate-solubilizing fungi; plant growth promotion ID CALCIUM-PHOSPHATE; PHOSPHORUS UPTAKE; MICROORGANISMS; FUNGI; PLANT; BACTERIA; STRAIN; ACIDS AB Alternative tactics for improving phosphorus nutrition in crop production are needed in China and elsewhere, as the overapplication of phosphatic fertilizers can adversely impact agricultural sustainability. Penicillium oxalicum P4 and Aspergillus niger P85 were isolated from a calcareous soil in China that had been exposed to excessive application of phosphatic fertilizer for decades. Each isolate excreted a number of organic acids into, acidified, and solubilized phosphorus in a synthetic broth containing insoluble tricalcium phosphate or rock phosphate. Isolate P4, applied as a seed treatment, increased maize fresh mass per plant when rock phosphate was added to the calcareous soil in greenhouse pot studies. Isolate P85 did not increase maize fresh mass per plant but did significantly increase total phosphorus per plant when rock phosphate was added. Significant increases in 7 and 4 organic acids were detected in soil in association with isolates P4 and P85, respectively, relative to the soil-only control. The quantity and (or) number of organic acids produced by these isolates increased when rock phosphate was added to the soil. Both isolates also significantly increased available phosphorus in soil in the presence of added rock phosphate and effectively colonized the maize rhizosphere. Studies reported here indicate that isolate P4 is adapted to and capable of promoting maize growth in a calcareous soil. Plant-growth promotion by this isolate is likely due, at least in part, to increased phosphorus availability resulting from the excretion of organic acids into, and the resulting acidification of, this soil. C1 [Yin, Zhongwei; Shi, Fachao; Jiang, Hongmei; Fan, Bingquan] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. [Yin, Zhongwei; Chen, Sanfeng] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China. [Roberts, Daniel P.] USDA ARS, Sustainable Agr Syst Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Roberts, DP (reprint author), USDA ARS, Sustainable Agr Syst Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM dan.roberts@ars.usda.gov; bqfan@caas.ac.cn FU National High Technology Research and Development of China [2013AA102802]; Key Projects in National Science and Technology Pillar Program during the 12th Five-year Plan Period [2011BAD11B03]; 948 Agricultural Project [2011-G25] FX We thank Laurie McKenna, Sustainable Agricultural Systems Laboratory, USDA-ARS, Beltsville, Maryland, for generation of the figures, and Bryan Vinyard, Biometrics Unit, USDA-ARS, Beltsville, Maryland, for statistical analysis. Work reported here was supported by grants from the National High Technology Research and Development of China program (2013AA102802), the Key Projects in National Science and Technology Pillar Program during the 12th Five-year Plan Period (2011BAD11B03), and the 948 Agricultural Project (2011-G25). NR 40 TC 3 Z9 3 U1 8 U2 33 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0008-4166 EI 1480-3275 J9 CAN J MICROBIOL JI Can. J. Microbiol. PD DEC PY 2015 VL 61 IS 12 BP 913 EP 923 DI 10.1139/cjm-2015-0358 PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology GA CZ3CW UT WOS:000366982800004 PM 26469739 ER PT J AU Hittinger, CT Rokas, A Bai, FY Boekhout, T Goncalves, P Jeffries, TW Kominek, J Lachance, MA Libkind, D Rosa, CA Sampaio, JP Kurtzman, CP AF Hittinger, Chris Todd Rokas, Antonis Bai, Feng-Yan Boekhout, Teun Goncalves, Paula Jeffries, Thomas W. Kominek, Jacek Lachance, Marc-Andre Libkind, Diego Rosa, Carlos A. Sampaio, Jose Paulo Kurtzman, Cletus P. TI Genomics and the making of yeast biodiversity SO CURRENT OPINION IN GENETICS & DEVELOPMENT LA English DT Article ID HORIZONTAL GENE-TRANSFER; SACCHAROMYCES-CEREVISIAE GENOME; LAGER-BREWING YEAST; POPULATION GENOMICS; DRAFT GENOME; INTERSPECIES HYBRID; CANDIDA-ALBICANS; SEQUENCE; EVOLUTION; REVEALS AB Yeasts are unicellular fungi that do not form fruiting bodies. Although the yeast lifestyle has evolved multiple times, most known species belong to the subphylum Saccharomycotina (syn. Hemiascomycota, hereafter yeasts). This diverse group includes the premier eukaryotic model system, Saccharomyces cerevisiae; the common human commensal and opportunistic pathogen, Candida albicans; and over 1000 other known species (with more continuing to be discovered). Yeasts are found in every biome and continent and are more genetically diverse than angiosperms or chordates. Ease of culture, simple life cycles, and small genomes (similar to 10-20 Mbp) have made yeasts exceptional models for molecular genetics, biotechnology, and evolutionary genomics. Here we discuss recent developments in understanding the genomic underpinnings of the making of yeast biodiversity, comparing and contrasting natural and human-associated evolutionary processes. Only a tiny fraction of yeast biodiversity and metabolic capabilities has been tapped by industry and science. Expanding the taxonomic breadth of deep genomic investigations will further illuminate how genome function evolves to encode their diverse metabolisms and ecologies. C1 [Hittinger, Chris Todd; Kominek, Jacek] Univ Wisconsin, Genet Lab, Genome Ctr Wisconsin, Wisconsin Energy Inst,JF Crow Inst Study Evolut, Madison, WI 53706 USA. [Hittinger, Chris Todd] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Bai, Feng-Yan; Boekhout, Teun] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China. [Boekhout, Teun] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands. [Boekhout, Teun] Second Mil Med Univ, Shanghai Key Lab Mol Med Mycol, Changzheng Hosp, Shanghai, Peoples R China. [Goncalves, Paula; Sampaio, Jose Paulo] Univ Nova Lisboa, Fac Ciencias & Tecnol, Dept Ciencias Vida, UCIBIO REQUIMTE, P-2829516 Caparica, Portugal. [Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Lachance, Marc-Andre] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada. [Libkind, Diego] Consejo Nacl Invest Cient & Tecn, UNComahue, Inst Invest Biodiversidad & Medioambiente INIBIOM, Lab Microbiol Aplicada & Biotecnol, San Carlos De Bariloche, Rio Negro, Argentina. [Rosa, Carlos A.] Univ Fed Minas Gerais, ICB, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Kurtzman, Cletus P.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. RP Hittinger, CT (reprint author), Univ Wisconsin, Genet Lab, Genome Ctr Wisconsin, Wisconsin Energy Inst,JF Crow Inst Study Evolut, Madison, WI 53706 USA. EM cthittinger@wisc.edu RI Sampaio, Jose Paulo/C-5532-2011; Goncalves, Paula/B-4016-2010; OI Goncalves, Paula/0000-0003-2103-1060; Kominek, Jacek/0000-0002-1916-0122; Sampaio, Jose/0000-0001-8145-5274; Rokas, Antonis/0000-0002-7248-6551 FU National Science Foundation [DEB-1442148, DEB-1253634, DEB-1442113]; DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494]; USDA National Institute of Food and Agriculture [1003258]; Alexander von Humboldt Foundation; Pew Charitable Trusts; National Institutes of Health (NIAID) [AI105619]; March of Dimes; Natural Science and Engineering Research Council of Canada; CONICET; Universidad Nacional del Comahue [B171]; FONCyT [PICT 2014-2542]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)-Brazil FX We thank the many colleagues who alerted us to relevant literature and apologize to those whose work was excluded due to space limitations or oversight. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. Research in the Hittinger Lab is supported by the National Science Foundation (Grant Nos. DEB-1442148 to CTH and CPK, DEB-1253634 to CTH); the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494); the USDA National Institute of Food and Agriculture (Hatch project 1003258); the Alexander von Humboldt Foundation (CTH is an Alfred Toepfer Faculty Fellow); and the Pew Charitable Trusts (CTH is a Pew Scholar in the Biomedical Sciences). Research in the Rokas Lab is supported by the National Science Foundation (DEB-1442113); the National Institutes of Health (NIAID, AI105619); and the March of Dimes. Research in the Lachance Lab is supported by the Natural Science and Engineering Research Council of Canada. Research in the Libkind Lab is supported by CONICET, Universidad Nacional del Comahue (B171), and FONCyT (PICT 2014-2542). Research in the Rosa Lab is supported by the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)-Brazil. NR 105 TC 13 Z9 13 U1 10 U2 33 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0959-437X EI 1879-0380 J9 CURR OPIN GENET DEV JI Curr. Opin. Genet. Dev. PD DEC PY 2015 VL 35 BP 100 EP 109 DI 10.1016/j.gde.2015.10.008 PG 10 WC Cell Biology; Genetics & Heredity SC Cell Biology; Genetics & Heredity GA CZ1XZ UT WOS:000366900600015 PM 26649756 ER PT J AU Fortin, M Beromi, MM Lai, A Tarves, PC Mullen, CA Boateng, AA West, NM AF Fortin, Michael Beromi, Megan Mohadjer Lai, Amy Tarves, Paul C. Mullen, Charles A. Boateng, Akwasi A. West, Nathan M. TI Structural Analysis of Pyrolytic Lignins Isolated from Switchgrass Fast-Pyrolysis Oil SO ENERGY & FUELS LA English DT Article ID WATER-INSOLUBLE FRACTION; NMR ANALYSIS; BIO-OIL; INFRARED SPECTRA; BIOMASS; WOOD; PRETREATMENT; PLANTS; TEMPERATURE; OLIGOMERS AB Structural characterization of lignin extracted from the bio-oil produced by fast pyrolysis of switchgrass (Panicum virgatum) is reported. This is important for understanding the utility of lignin as a chemical feedstock in a pyrolysis-based biorefinery scheme. Pyrolysis induces a variety of structural changes to lignin in addition to reduction in molecular weight. The guaiacol structural units remain largely intact, and some hemicellulose stays covalently linked to the lignin. However, two-dimensional H-1-C-13 HSQC NMR analysis shows an absence of y-methylene hydrogens from beta-O-4 linkages, implying that rearrangements in the propyl linking chains have occurred. Peculate and hydroxyl phenol esters are still present in the pyrolyzed lignin, but at lower concentrations than in unpyrolyzed switchgrass lignin. C1 [Fortin, Michael; Beromi, Megan Mohadjer; Lai, Amy; Tarves, Paul C.; West, Nathan M.] Allegheny Univ Hlth Sci, Dept Chem & Biochem, Philadelphia, PA 19104 USA. [Mullen, Charles A.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP West, NM (reprint author), Eastman Chem Co, 200 S Wilcox Dr, Kingsport, TN 37660 USA. EM nedwest@eastman.com FU U.S. Department of Agriculture Biomass Research and Development Initiative (BRDI) [USDA-NIFA 2012-10008-20271] FX We acknowledge funding support from U.S. Department of Agriculture Biomass Research and Development Initiative (BRDI) grant (USDA-NIFA 2012-10008-20271). NR 51 TC 5 Z9 5 U1 5 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD DEC PY 2015 VL 29 IS 12 BP 8017 EP 8026 DI 10.1021/acs.energyfuels.5b01726 PG 10 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA CZ1PO UT WOS:000366878000029 ER PT J AU Jones, K Ramakrishnan, G Uchimiya, M Orlov, A Castaldi, MJ LeBlanc, J Hiradate, S AF Jones, Keith Ramakrishnan, Girish Uchimiya, Minori Orlov, Alexander Castaldi, Marco J. LeBlanc, Jeffrey Hiradate, Syuntaro TI Fate of Higher-Mass Elements and Surface Functional Groups during the Pyrolysis of Waste Pecan Shell SO ENERGY & FUELS LA English DT Article ID CARBON-DIOXIDE; ORGANIC-COMPOUNDS; BIOCHAR; SOIL; MICROTOMOGRAPHY; SEQUESTRATION; SPECTROSCOPY; TEMPERATURE; FEEDSTOCK; CHAR AB Thermochemical conversion of agricultural wastes to bioenergy has a potential to play forefront roles within the context of the food, energy, and water nexus. The biochar solid product of pyrolysis is a promising tool to manage food crop production and water resources by means of soil amendment. The goal of this study was to understand the fate of surface functional groups and higher-atomic-mass elements during the pyrolysis of pecan shell, which is known to accumulate calcium oxalate. Pecan shell feedstock and biochars were analyzed ex situ using X-ray computed microtomography and solid-state C-13 cross-polarization and magic-angle-spinning NMR spectroscopy; the pyrolysis kinetics was monitored in situ by thermogravimetric analysis gas chromatography (TGA-GC). The NMR spectra indicated the greatest (i) reduction in O/N alkyl functionality and (ii) increase in the aromatic peak between 300 and 500 degrees C. Primary physical transformation was observed near 400 degrees C in the tomography slice images and corresponding attenuation coefficients. Key changes in physical structure (microtomography) as well as chemical constituents (solid-state NMR) of pecan shell at 300-500 degrees C coincided with the evolution of gaseous products (hydrogen, methane, carbon monoxide, carbon dioxide, ethylene, and ethane, as monitored in situ by TGA-GC) occurring at 200-500 degrees C. These observations followed the reported (0 formation and removal of carboxyl surface functional groups of biochar and (ii) conversion of calcium oxalate to carbonate, both occurring at the key transition temperature near 400 degrees C. Combined with the mass balance (99.7%) obtained for gas-, liquid-, and solid-phase products, these findings will facilitate reactor design to optimize syngas and bio-oil yields and manipulate the surface reactivity of biochar soil amendment. C1 [Jones, Keith; Ramakrishnan, Girish; Uchimiya, Minori; Orlov, Alexander] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA. [Jones, Keith] Brookhaven Natl Lab, Biol Environm & Climate Sci Dept, Upton, NY 11973 USA. [Uchimiya, Minori] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Castaldi, Marco J.; LeBlanc, Jeffrey] CUNY City Coll, Dept Chem Engn, New York, NY 10031 USA. [Hiradate, Syuntaro] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan. RP Uchimiya, M (reprint author), SUNY Stony Brook, Dept Mat Sci & Engn, Room 314 Old Engn, Stony Brook, NY 11794 USA. EM sophie.uchimiya@ars.usda.gov NR 40 TC 2 Z9 2 U1 6 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD DEC PY 2015 VL 29 IS 12 BP 8095 EP 8101 DI 10.1021/acs.energyfuels.5b02428 PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA CZ1PO UT WOS:000366878000039 ER PT J AU Reed, SE Juzwik, J English, JT Ginzel, MD AF Reed, Sharon E. Juzwik, Jennifer English, James T. Ginzel, Matthew D. TI Colonization of Artificially Stressed Black Walnut Trees by Ambrosia Beetle, Bark Beetle, and Other Weevil Species (Coleoptera: Curculionidae) in Indiana and Missouri SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE scolytine; platypodine; Geosmithia morbida; Juglans nigra; thousand cankers disease ID XYLOSANDRUS-GERMANUS COLEOPTERA; SOUTHEASTERN UNITED-STATES; SCOLYTIDAE COLEOPTERA; FLIGHT PATTERNS; NORTH-AMERICA; ETHANOL; ATTACK; PINE; US; PLATYPODIDAE AB Thousand cankers disease (TCD) is a new disease of black walnut (Juglans nigra L.) in the eastern United States. The disease is caused by the interaction of the aggressive bark beetle Pityophthorus juglandis Blackman and the canker-forming fungus, Geosmithia morbida M. Kolarik, E. Freeland, C. Utley & Tisserat, carried by the beetle. Other insects also colonize TCD-symptomatic trees and may also carry pathogens. A trap tree survey was conducted in Indiana and Missouri to characterize the assemblage of ambrosia beetles, bark beetles, and other weevils attracted to the main stems and crowns of stressed black walnut. More than 100 trees were girdled and treated with glyphosate (Riverdale Razor Pro, Burr Ridge, Illinois) at 27 locations. Nearly 17,000 insects were collected from logs harvested from girdled walnut trees. These insects represented 15 ambrosia beetle, four bark beetle, and seven other weevil species. The most abundant species included Xyleborinus saxeseni Ratzburg, Xylosandrus crassiusculus Motschulsky, Xylosandrus germanus Blandford, Xyleborus affinis Eichhoff, and Stenomimus pallidus Boheman. These species differed in their association with the stems or crowns of stressed trees. Multiple species of insects were collected from individual trees and likely colonized tissues near each other. At least three of the abundant species found (S. pallidus, X. crassiusculus, and X. germanus) are known to carry propagules of canker-causing fungi of black walnut. In summary, a large number of ambrosia beetles, bark beetles, and other weevils are attracted to stressed walnut trees in Indiana and Missouri. Several of these species have the potential to introduce walnut canker pathogens during colonization. C1 [Reed, Sharon E.; English, James T.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Juzwik, Jennifer] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Ginzel, Matthew D.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Reed, SE (reprint author), Univ Missouri, Plant Sci Div, 108 Waters Hall, Columbia, MO 65211 USA. EM reedsh@missouri.edu FU USDA-Forest Service Forest Health Protection Special Projects grant [11-CR-11242310-062] FX We thank Simeon Wright (Missouri Department of Conservation), Jerry Van Sambeek (USDA-Forest Service), Harlan Palm, and other Walnut Council members, Jim Licklider, Keith Brown, and numerous members of the Purdue Hardwood Tree Improvement and Regeneration Center (Jim McKenna, Lenny Farlee, Brian Beheler) for assistance with site selection, collection of wood, and facilities. We also thank Megan Shawgo, Matt Paschen, and Gary Frasier for assistance with collection and sorting of emerged insects. In addition, Bill Dijak of the USDA-Forest Service provided mapping support, and Mark Ellersieck of the University of Missouri provided statistical support. This project was funded by a USDA-Forest Service Forest Health Protection Special Projects grant (11-CR-11242310-062). NR 65 TC 2 Z9 2 U1 5 U2 21 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD DEC PY 2015 VL 44 IS 6 BP 1455 EP 1464 DI 10.1093/ee/nvv126 PG 10 WC Entomology SC Entomology GA CZ1AS UT WOS:000366838700001 PM 26314028 ER PT J AU Peterson, DL Duan, JJ Yaninek, JS Ginzel, MD Sadof, CS AF Peterson, Donnie L. Duan, Jian J. Yaninek, J. S. Ginzel, Matthew D. Sadof, Clifford S. TI Growth of Larval Agrilus planipennis (Coleoptera: Buprestidae) and Fitness of Tetrastichus planipennisi (Hymenoptera: Eulophidae) in Blue Ash (Fraxinus quadrangulata) and Green Ash (F. pennsylvanica) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Agrilus planipennis; Fraxinus; Tetrastichus planipennisi; blue ash; green ash ID PARASITOID COTESIA-MELANOSCELA; EMERALD ASH; BORER COLEOPTERA; NORTH-AMERICAN; LIFE-HISTORY; LEAF BEETLE; FAIRMAIRE COLEOPTERA; POPULATION-DYNAMICS; SPECIES RESISTANT; NATURAL ENEMIES AB Emerald ash borer (Agrilus planipennis Fairmaire) is an invasive primary pest of North American ash (Fraxinus spp.) trees. Blue ash (F. quadrangulata) is less susceptible to emerald ash borer infestations in the forest than other species of North American ash. Whereas other studies have examined adult host preferences, we compared the capacity of emerald ash borer larvae reared from emerald ash borer eggs in the field and in the laboratory to survive and grow in blue ash and the more susceptible green ash (F. pennsylvanica). Emerald ash borer larval survivorship was the same on both ash species. Mortality due to wound periderm formation was only observed in living field grown trees, but was low (< 4%) in both green and blue ash. No difference in larval mortality in the absence of natural enemies suggests that both green and blue ash can support the development of emerald ash borer. Larvae reared from eggs on blue ash were smaller than on green ash growing in the field and also in bolts that were infested under laboratory conditions. In a laboratory study, parasitism rates of confined Tetrastichus planipennisi were similar on emerald ash borer larvae reared in blue and green ash bolts, as were fitness measures of the parasitoid including brood size, sex ratio, and adult female size. Thus, we postulate that emerald ash borer larvae infesting blue ash could support populations of T. planipennisi and serve as a potential reservoir for this introduced natural enemy after most of the other native ash trees have been killed. C1 [Peterson, Donnie L.; Yaninek, J. S.; Ginzel, Matthew D.; Sadof, Clifford S.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. RP Sadof, CS (reprint author), Purdue Univ, Dept Entomol, 901 W State St, W Lafayette, IN 47907 USA. EM csadof@purdue.edu FU USDA APHIS [13-8218-0871-CA-270] FX Thanks to Adam Witte, Carlos Quesada Machiqua, and Octavio Menocal for experiment set-up and data collection. Thanks to Leah Bauer for providing emerald ash borer eggs. The city of West Lafayette and Indiana Department of Natural Resources provided field sites. The parasitoids were produced and supplied by the USDA Emerald Ash Borer Parasitoid Rearing Facility in Brighton, MI. This research was in partial fulfillment of a MS degree for D.L.P. from Purdue University. Funding for this project was provided in part by USDA APHIS Cooperative Agreement 13-8218-0871-CA-270 to CS. NR 60 TC 1 Z9 1 U1 1 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD DEC PY 2015 VL 44 IS 6 BP 1512 EP 1521 DI 10.1093/ee/nvv122 PG 10 WC Entomology SC Entomology GA CZ1AS UT WOS:000366838700006 PM 26314024 ER PT J AU Wang, L Zeng, L Chen, J AF Wang, Lei Zeng, Ling Chen, Jian TI Sublethal Effect of Imidacloprid on Solenopsis invicta (Hymenoptera: Formicidae) Feeding, Digging, and Foraging Behavior SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Solenopsis invicta; neonicotinoid; sublethal concentration; feeding; recruitment ID EXPOSURE; SOILS; BEES; RHINOTERMITIDAE; CHLORDIMEFORM; PERSISTENCE; MORTALITY; HONEYBEES; TOXICITY; ISOPTERA AB There is increasing evidence that exposure to neonicotinoid insecticides at sublethal levels impairs colonies of honeybees and other pollinators. Recently, it was found that sublethal contamination with neonicotinoids also affect growth and behavior of ants. In this study, we exposed red imported fire ants, Solenopsis invicta Buren, to sublethal dosages of dietary imidacloprid and investigated its effect on ant feeding, digging, and foraging behavior. S. invicta consumed significantly more sugar water containing 0.01 mu g/ml imidacloprid than untreated sugar water. Ants fed with 0.01 mu g/ml imidacloprid also showed significantly increased digging activity than ants fed with untreated sugar water. However, imidacloprid at >= 0.25 mu g/ml significantly suppressed sugar water consumption, digging, and foraging behavior. These results indicate that imidacloprid at sublethal concentrations may have a significant and complicated effect on S. invicta. C1 [Wang, Lei; Zeng, Ling] South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China. [Chen, Jian] ARS, Natl Biol Control Lab, Southeast Area, USDA, Stoneville, MS 38776 USA. RP Chen, J (reprint author), ARS, Natl Biol Control Lab, Southeast Area, USDA, 59 Lee Rd, Stoneville, MS 38776 USA. EM jian.chen@ars.usda.gov FU Hesheng Zhujiang Education Foundation; South China Agricultural University; Post-graduates Study Abroad Program, China Scholarship Council FX We thank Dr. Abbas Ali, School of Pharmacy, The University of Mississippi, Oxford, MS, and Dr. Beverly Wiltz, Delgado Community College, New Orleans, LA, for critical reviews of the manuscript. We thank Mr. Leon Hicks and Ms. Weihua Jiang for their technical assistance. This project is supported by the Hesheng Zhujiang Education Foundation, South China Agricultural University and the Post-graduates Study Abroad Program, China Scholarship Council. NR 34 TC 1 Z9 1 U1 10 U2 24 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD DEC PY 2015 VL 44 IS 6 BP 1544 EP 1552 DI 10.1093/ee/nvv127 PG 9 WC Entomology SC Entomology GA CZ1AS UT WOS:000366838700010 PM 26314029 ER PT J AU Arsenault, AL Havill, NP Mayfield, AE Wallin, KF AF Arsenault, Arielle L. Havill, Nathan P. Mayfield, Albert E. Wallin, Kimberly F. TI Behavioral Responses of Laricobius spp. and Hybrids (Coleoptera: Derodontidae) to Hemlock Woolly Adelgid and Adelgid Host Tree Odors in an Olfactometer SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE biological control; host location behavior; Laricobius spp.; hybridization; Tsuga canadensis ID BIOLOGICAL-CONTROL AGENT; EASTERN NORTH-AMERICA; LONGITARSUS-JACOBAEAE; HEMIPTERA ADELGIDAE; PACIFIC-NORTHWEST; NIGRINUS; PREDATOR; HYBRIDIZATION; PARASITOIDS; HERBIVORES AB The predatory species Laricobius nigrinus (Fender) and Laricobius osakensis (Shiyake and Montgomery) (Coleoptera: Derodontidae) have been released for biological control of hemlock woolly adelgid (Adelges tsugae; Hemiptera: Adelgidae) in eastern North America. L. osakensis is native to Japan, whereas L. nigrinus is endemic to the Pacific Northwest of the United States and Canada. After release, L. nigrinus was found to hybridize with the native eastern species, Laricobius rubidus (LeConte). The purpose of this study is to observe prey location behaviors of these three Laricobius species and L. nigrinus x L. rubidus (Ln x Lr) hybrids. Olfactometer bioassays were used to test response to host odors of adelgid-infested eastern hemlock, uninfested eastern hemlock, and uninfested eastern white pine. Predators reacted in the olfactometer more quickly when adelgid-infested foliage was included as a choice. L. nigrinus preferred infested eastern hemlock over uninfested eastern white pine, and L. rubidus preferred uninfested eastern white pine over uninfested eastern hemlock. Laricobius hybrids did not show a preference for foliage types known to be primary adelgid hosts (eastern hemlock and eastern white pine). Unequal preference by species of Laricobius for host trees of different adelgid prey could therefore be maintaining Laricobius species barriers despite hybridization. L. osakensis for this study were reared in the laboratory, whereas other species in this study were collected from the field, yet still were attracted to infested and uninfested eastern hemlock. This species also responded most quickly in the olfactometer, which is encouraging for successful biological control with this species. C1 [Arsenault, Arielle L.; Wallin, Kimberly F.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Havill, Nathan P.; Wallin, Kimberly F.] US Forest Serv, USDA, No Res Stn, Hamden, CT 06513 USA. [Mayfield, Albert E.] US Forest Serv, USDA, Southern Res Stn, Asheville, NC 28804 USA. RP Wallin, KF (reprint author), Univ Vermont, Rubenstein Sch Environm & Nat Resources, 81 Carrigan Dr, Burlington, VT 05405 USA. EM kwallin@uvm.edu FU University of Vermont; USDA Forest Service Northern Research Station; USDA Forest Service, Forest Health Technology Enterprise Team, Morgantown, WV; USDA Forest Service, Southern Research Station FX We gratefully acknowledge support from The University of Vermont and USDA Forest Service Northern Research Station. Funding for this research was from USDA Forest Service, Forest Health Technology Enterprise Team, Morgantown, WV, and USDA Forest Service, Southern Research Station. We thank Dr. Richard McDonald (Symbiont Biological Pest Management), Mark Dulusky (University of Georgia), Andrew Tait and Barbara Reynolds (University of North Carolina Asheville), Carrie Jubb (Virginia Tech), and Dr. Darrell Ross (Oregon State University) for their assistance in coordinating, collecting, and shipping insect and plant material. We thank DeAndra Newman for technical assistance. We thank Drs. Lori Stevens and Jon Erickson (University of Vermont) and five reviewers for providing useful comments that strengthened the manuscript. NR 58 TC 1 Z9 1 U1 6 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD DEC PY 2015 VL 44 IS 6 BP 1562 EP 1570 DI 10.1093/ee/nvv135 PG 9 WC Entomology SC Entomology GA CZ1AS UT WOS:000366838700012 PM 26314036 ER PT J AU Stephens, AR Asplen, MK Hutchison, WD Venette, RC AF Stephens, A. R. Asplen, M. K. Hutchison, W. D. Venette, R. C. TI Cold Hardiness of Winter-Acclimated Drosophila suzukii (Diptera: Drosophilidae) Adults SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE spotted wing drosophila; Drosophila melanogaster; cold acclimation; cold tolerance ID SPOTTED-WING DROSOPHILA; UNITED-STATES; PHENOTYPIC PLASTICITY; MELANOGASTER; TOLERANCE; INFESTATION; POPULATION; ADAPTATION; EVOLUTION; FRUITS AB Drosophila suzukii Matsumura, often called spotted wing drosophila, is an exotic vinegar fly that is native to Southeast Asia and was first detected in the continental United States in 2008. Previous modeling studies have suggested that D. suzukii might not survive in portions of the northern United States or southern Canada due to the effects of cold. As a result, we measured two aspects of insect cold tolerance, the supercooling point and lower lethal temperature, for D. suzukii summer-morph pupae and adults and winter-morph adults. Supercooling points were compared to adults of Drosophila melanogaster Meigen. The lower lethal temperature of D. suzukii winter-morph adults was significantly colder than that for D. suzukii summer-morph adults, while supercooling points of D. suzukii winter-morph adults were actually warmer than that for D. suzukii summer-morph adults and pupae. D. suzukii summer-morph adult supercooling points were not significantly different than those for D. melanogaster adults. These measures indicate that D. suzukii is a chill intolerant insect, and winter-morph adults are the most cold-tolerant life stage. These results can be used to improve predictions of where D. suzukii might be able to establish overwintering populations and cause extensive damage to spring fruit crops. C1 [Stephens, A. R.; Hutchison, W. D.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Asplen, M. K.] Metropolitan State Univ, Dept Nat Sci, St Paul, MN 55106 USA. [Venette, R. C.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. RP Venette, RC (reprint author), US Forest Serv, USDA, No Res Stn, 1561 Lindig St, St Paul, MN 55108 USA. EM rvenette@fs.fed.us FU Minnesota Agricultural Experiment Station Rapid Agricultural Response Fund FX We thank T. Neufeld and R. Isaacs lab group for supplying D. melanogaster and D. suzukii, respectively. We thank James R. Venette for the design and construction of the dowels to support thermocouples and confine insects. We also thank Emily Hoover, Chris Phillips, and Eric Burkness for constructive conversations. This work was funded by Minnesota Agricultural Experiment Station Rapid Agricultural Response Fund. NR 40 TC 10 Z9 10 U1 9 U2 56 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD DEC PY 2015 VL 44 IS 6 BP 1619 EP 1626 DI 10.1093/ee/nvv134 PG 8 WC Entomology SC Entomology GA CZ1AS UT WOS:000366838700018 PM 26317777 ER PT J AU Robinson, BR Sathuvalli, V Bamberg, J Goyer, A AF Robinson, Bruce R. Sathuvalli, Vidyasagar Bamberg, John Goyer, Aymeric TI Exploring Folate Diversity in Wild and Primitive Potatoes for Modern Crop Improvement SO GENES LA English DT Article DE vitamin B-9; folate; biofortification; potato; Solanum tuberosum; andigenum; vernei ID ONE-CARBON METABOLISM; LIQUID-CHROMATOGRAPHY; FOLIC-ACID; MICROBIOLOGICAL ASSAY; TRIENZYME EXTRACTION; POPULATION; BIOFORTIFICATION; DERIVATIVES; DEPRESSION; PLANTS AB Malnutrition is one of the world's largest health concerns. Folate (also known as vitamin B-9) is essential in the human diet, and without adequate folate intake, several serious health concerns, such as congenital birth defects and an increased risk of stroke and heart disease, can occur. Most people's folate intake remains sub-optimal, even in countries that have a folic acid food fortification program in place. Staple crops, such as potatoes, represent an appropriate organism for biofortification through traditional breeding based on their worldwide consumption and the fact that modern cultivars only contain about 6% of the daily recommended intake of folate. To start breeding potatoes with enhanced folate content, high folate potato material must be identified. In this study, 250 individual plants from 77 accessions and 10 Solanum species were screened for their folate content using a tri-enzyme extraction and microbial assay. There was a 10-fold range of folate concentrations among individuals. Certain individuals within the species Solanum tuberosum subsp. andigenum, Solanum vernei and Solanum boliviense have the potential to produce more than double the folate concentrations of commercial cultivars, such as Russet Burbank. Our results show that tapping into the genetic diversity of potato is a promising approach to increase the folate content of this important crop. C1 [Robinson, Bruce R.; Sathuvalli, Vidyasagar; Goyer, Aymeric] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [Robinson, Bruce R.; Sathuvalli, Vidyasagar] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97330 USA. [Bamberg, John] USDA ARS, Sturgeon Bay, WI 54235 USA. [Goyer, Aymeric] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. RP Goyer, A (reprint author), Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. EM Bruce.Robinson@oregonstate.edu; Vidyasagar@oregonstate.edu; john.bamberg@ars.usda.gov; aymeric.goyer@oregonstate.edu FU National Needs Graduate Student Fellowship from the USDA National Institute of Food and Agriculture; USDA Western Sustainable Agriculture Research and Education FX We thank Matthew Warman, Mark Barnett and Solomon Yilma for technical help. Bruce Robinson was supported by a National Needs Graduate Student Fellowship from the USDA National Institute of Food and Agriculture and a Fellowship from the USDA Western Sustainable Agriculture Research and Education. NR 27 TC 0 Z9 0 U1 3 U2 15 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4425 J9 GENES-BASEL JI Genes PD DEC PY 2015 VL 6 IS 4 BP 1300 EP 1314 DI 10.3390/genes6041300 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA CZ3OG UT WOS:000367013300020 PM 26670256 ER PT J AU Landry, A Madson, M Thomson, J Zoellner, J Connell, C Yadrick, K AF Landry, Alicia Madson, Michael Thomson, Jessica Zoellner, Jamie Connell, Carol Yadrick, Kathleen TI A randomized trial using motivational interviewing for maintenance of blood pressure improvements in a community-engaged lifestyle intervention: HUB city steps SO HEALTH EDUCATION RESEARCH LA English DT Article ID PHYSICAL-ACTIVITY; AFRICAN-AMERICAN; METAANALYSIS; HYPERTENSION AB Little is known about the effective dose of motivational interviewing for maintaining intervention-induced health outcome improvements. The purpose of this study was to compare effects of two doses of motivational interviewing for maintaining blood pressure improvements in a community-engaged lifestyle intervention conducted with African-Americans. Participants were tracked through a 12-month maintenance phase following a 6-month intervention targeting physical activity and diet. For the maintenance phase, participants were randomized to receive a low (4) or high (10) dose of motivational interviewing delivered via telephone by trained research staff. Generalized linear models were used to test for group differences in blood pressure. Blood pressure significantly increased during the maintenance phase. No differences were apparent between randomized groups. Results suggest that 10 or fewer motivational interviewing calls over a 12-month period may be insufficient to maintain post-intervention improvements in blood pressure. Further research is needed to determine optimal strategies for maintaining changes. C1 [Landry, Alicia] Univ Cent Arkansas, Dept Family & Consumer Sci, Conway, AR 72035 USA. [Madson, Michael] Univ So Mississippi, Dept Psychol, Hattiesburg, MS 39406 USA. [Thomson, Jessica] Louisiana State Univ, Human Nutr & Food, USDA Agr Res Serv, Baton Rouge, LA 70803 USA. [Zoellner, Jamie] Virginia Polytech Inst & State Univ, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. [Connell, Carol; Yadrick, Kathleen] Univ So Mississippi, Dept Nutr & Food Syst, Hattiesburg, MS 39406 USA. RP Landry, A (reprint author), Univ Cent Arkansas, Dept Family & Consumer Sci, 201 Donaghey Ave, Conway, AR 72035 USA. EM alandry@uca.edu FU National Institute on Minority Health and Health Disparities (NIMHD) [R24MD002787] FX National Institute on Minority Health and Health Disparities (NIMHD) (R24MD002787). NR 41 TC 1 Z9 1 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0268-1153 EI 1465-3648 J9 HEALTH EDUC RES JI Health Educ. Res. PD DEC PY 2015 VL 30 IS 6 BP 910 EP 922 DI 10.1093/her/cyv058 PG 13 WC Education & Educational Research; Public, Environmental & Occupational Health SC Education & Educational Research; Public, Environmental & Occupational Health GA CZ5UG UT WOS:000367166900007 PM 26590242 ER PT J AU Singh, A Ramaswamy, HS AF Singh, Ajaypal Ramaswamy, H. S. TI High pressure modification of egg components: Exploration of calorimetric, structural and functional characteristics SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Article DE High pressure; Protein denaturation; Elastic modulus; Viscous modulus; Flow behavior; Foaming ID HIGH-HYDROSTATIC-PRESSURE; LIQUID WHOLE EGG; RHEOLOGICAL CHARACTERISTICS; EMULSIFYING PROPERTIES; WHITE PROTEINS; YOLK; DENATURATION; INACTIVATION; DISSOCIATION; OVALBUMIN AB The effects of high pressure (HP) treatment on the thermal, rheological and functional properties of various egg components (whole liquid egg and liquid egg white) were studied as a function of increasing pressure level and treatment time. Differential scanning calorimetry (DSC) and rheometric analysis were utilized to evaluate the extent of protein denaturation and liquid-gel transformation details (G', G ''). Overall, HP-treated samples exhibited predominantly solid-like (G' > G '') behavior in the frequency range employed (0.1-10 Hz). Pressure level (350-550 MPa) and treatment time (5-15 min) contributed significantly towards modification of liquid samples to partial gel formation. The highest level of pressure treatment (550 MPa for 15 min) was sufficient to cause complete gelatinization. Egg components exhibited a gradual liquid-solid gel transformation as they coagulated/denatured. Corresponding changes were observed in the flow behavior with increasing consistency behavior (m value) and decreasing flow behavior index (n value) showing pseudoplastic behavior. Functional properties like foaming ability, color and viscosity changed with alteration of rheological/structural properties for liquid egg white; however, whole liquid egg showed fewer significant changes (p < 0.05). Industrial relevance: Egg is known as a multifunctional food ingredient, and it plays a significant role in improving the functional profile of other finished foods. High-pressure processing can change the flow behaviour and structure by causing denaturation of proteins. These structural changes further induce transformation in the functional behavior of eggs like color, foaming and viscosity that is of prime importance in the food industry. Hence, evaluation of pressure processing conditions for modifying the egg functionality will help in developing egg and egg products with improved functionality. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Singh, Ajaypal; Ramaswamy, H. S.] McGill Univ, Dept Food Sci & Agr Chem, Montreal, PQ, Canada. RP Singh, A (reprint author), Agr Res Serv USA, USDA, 600 Mermaid Lane, Wyndmoor, PA 19038 USA. EM Ajaypal.singh@mail.mcgill.ca FU Discovery Grants Program of the Natural Sciences and Engineering Research Council of Canada FX This research was partially funded by the Discovery Grants Program of the Natural Sciences and Engineering Research Council of Canada. NR 56 TC 0 Z9 0 U1 8 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD DEC PY 2015 VL 32 BP 45 EP 55 DI 10.1016/j.ifset.2015.09.010 PG 11 WC Food Science & Technology SC Food Science & Technology GA CY9ZF UT WOS:000366764200006 ER PT J AU Zhang, QR Nachman, RJ Kaczmarek, K Kierus, K Zabrocki, J Denlinger, DL AF Zhang, Qirui Nachman, Ronald J. Kaczmarek, Krzysztof Kierus, Krzysztof Zabrocki, Janusz Denlinger, David L. TI Development of neuropeptide analogs capable of traversing the integument: A case study using diapause hormone analogs in Helicoverpa zea SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Diapause hormone; Agonists; Topical application; Diapause prevention ID BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE; CORN-EARWORM; TOPICAL APPLICATION; PROTHORACICOTROPIC HORMONE; INSECT NEUROPEPTIDES; HELIOTHIS-VIRESCENS; TOBACCO BUDWORM; PUPAL DIAPAUSE; EXPRESSION; GENE AB Diapause hormone and its analogs terminate pupal diapause in Helicovetpa zea when injected, but if such agents are to be used as effective diapause disruptors it will be essential to develop simple techniques for administering active compounds that can exert their effect by penetrating the insect epidermis. In the current study, we used two molecules previously shown to have high diapause-terminating activity as lead molecules to rationally design and synthesize new amphiphilic compounds with modified hydrophobic components. An assay for diapause termination identified 13 active compounds With EC50's ranging from 0.9 to 46.0 pmol per pupa. Three compounds, Decyl-1963, Dodecyl-1967, and Heptyl-1965, selected from the 13 compounds most active in breaking diapause following injection, also successfully prevented newly-formed pupae from entering diapause when applied topically. These compounds feature straight-chain, aliphatic hydrocarbons from 7 to 12 carbons in length; DH analogs with either a short-chain length of 4 or an aromatic phenethyl group failed to act topically. Compared to a high diapause incidence of 80-90% in controls, diapause incidence in pupae receiving a 10 nmole topical application of Decyl-1963, Dodecyl-1967, or Heptyl-1965 dropped to 30-45%. Decyl-1963 and Dodecyl-1967 also remained effective when topically applied at the 1 nmole level. These results suggest the feasibility of developing DH agonists that can be applied topically and suggest the identity of new lead molecules for development of additional topically-active DH analogs. The ability to penetrate the insect epidermis and/or midgut lining is critical if such agents are to be considered for future use as pest management tools. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Qirui; Denlinger, David L.] Ohio State Univ, Dept Entomol, Columbus, OH 43210 USA. [Zhang, Qirui; Denlinger, David L.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Nachman, Ronald J.; Kaczmarek, Krzysztof; Kierus, Krzysztof; Zabrocki, Janusz] USDA ARS, Insect Control & Cotton Dis Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Nachman, Ronald J.; Kaczmarek, Krzysztof; Kierus, Krzysztof; Zabrocki, Janusz] Lodz Univ Technol, Inst Organ Chem, PL-90924 Lodz, Poland. RP Nachman, RJ (reprint author), Ohio State Univ, Dept Entomol, 318 West 12th Ave, Columbus, OH 43210 USA. EM Nachman@tamu.edu; Denlinger.1@osu.edu OI Zhang, Qirui/0000-0002-2749-9740 FU USDA-NIFA [2011-67013-30199] FX This project was supported by USDA-NIFA Grant No. 2011-67013-30199. NR 21 TC 2 Z9 2 U1 2 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD DEC PY 2015 VL 67 SI SI BP 87 EP 93 DI 10.1016/j.ibmb.2015.02.015 PG 7 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CZ0BY UT WOS:000366771300011 PM 25753318 ER PT J AU Cho, E Zhang, XH Townsend, MK Selhub, J Paul, L Rosner, B Fuchs, CS Willett, WC Giovannucci, EL AF Cho, Eunyoung Zhang, Xuehong Townsend, Mary K. Selhub, Jacob Paul, Ligi Rosner, Bernard Fuchs, Charles S. Willett, Walter C. Giovannucci, Edward L. TI Unmetabolized Folic Acid in Prediagnostic Plasma and the Risk for Colorectal Cancer SO JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID POSTMENOPAUSAL WOMEN; FOLATE; FORTIFICATION; SERUM; HOMOCYSTEINE; FOOD AB Higher folate has been associated with a reduced colorectal cancer (CRC) risk, but excessive folate may promote tumor progression. The role of unmetabolized folic acid (UFA) from high folic acid consumption in carcinogenesis is largely unexplored. We evaluated prediagnostic plasma levels of UFA in relation to CRC risk in nested case-control studies (618 CRC case patients and 1207 matched control) with blood samples collected prior to folic acid fortification. UFA was detected in 21.4% of control UFA levels were not associated with CRC risk. Compared with undetectable levels, the multivariable relative risks (RRs) of CRC were 1.03 (95% confidence interval [CI] = 0.73 to 1.46) for less than 0.5 nmol/L and 1.12 (95% CI = 0.81 to 1.55) for 0.5 nmol/L or more (P-trend = .32). A positive association between UFA levels and CRC risk was observed among men (RR = 1.57, 95% CI = 0.99 to 2.49 for >= 0.5 nmol/L vs undetectable, P-interaction =.04), and a positive association was also observed among those with the methylene-tetrahydrofolate reductase (MTHFR) CT/TT genotype (RR = 2.20, 95% CI = 1.22 to 3.94 for >= 0.5 nmol/L vs undetectable, P-interaction = 0.02). In conclusion, prediagnostic plasma levels of UFA from the prefortification period were not associated with risk of CRC. C1 [Cho, Eunyoung; Zhang, Xuehong; Townsend, Mary K.; Rosner, Bernard; Willett, Walter C.; Giovannucci, Edward L.] Brigham & Womens Hosp, Dept Med, Channing Div Network Med, Boston, MA 02115 USA. [Cho, Eunyoung; Zhang, Xuehong; Townsend, Mary K.; Rosner, Bernard; Fuchs, Charles S.; Willett, Walter C.; Giovannucci, Edward L.] Harvard Univ, Sch Med, Boston, MA USA. [Cho, Eunyoung] Brown Univ, Warren Alpert Med Sch, Dept Dermatol, Providence, RI 02912 USA. [Cho, Eunyoung] Brown Univ, Sch Publ Hlth, Dept Epidemiol, Providence, RI 02912 USA. [Selhub, Jacob; Paul, Ligi] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rosner, Bernard] Harvard Univ, TH Chan Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Willett, Walter C.; Giovannucci, Edward L.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Willett, Walter C.; Giovannucci, Edward L.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Fuchs, Charles S.] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA. RP Cho, E (reprint author), Brown Univ, Warren Alpert Med Sch, Dept Dermatol, Box G-D, Providence, RI 02912 USA. EM eunyoung_cho@brown.edu FU National Institutes of Health [CA136950, CA87969, CA49449, CA167552] FX This work was supported by research grants CA136950 (to EC), CA87969, CA49449, and CA167552 (to WCW) from the National Institutes of Health. NR 22 TC 4 Z9 4 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0027-8874 EI 1460-2105 J9 JNCI-J NATL CANCER I JI JNCI-J. Natl. Cancer Inst. PD DEC PY 2015 VL 107 IS 12 AR djv260 DI 10.1093/jnci/djv260 PG 4 WC Oncology SC Oncology GA CZ2YN UT WOS:000366970900005 PM 26376686 ER PT J AU Holt, RR Yim, SJ Shearer, GC Hackman, RM Djurica, D Newman, JW Shindel, AW Keen, CL AF Holt, Roberta R. Yim, Sun J. Shearer, Gregory C. Hackman, Robert M. Djurica, Dragana Newman, John W. Shindel, Alan W. Keen, Carl L. TI Effects of short-term walnut consumption on human microvascular function and its relationship to plasma epoxide content SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Walnuts; Vascular function; Oxylipin; alpha-Linolenic acid; Linoleic acid ID CARDIOVASCULAR RISK-FACTORS; FLOW-MEDIATED DILATION; ALPHA-LINOLENIC ACID; HIGH-FAT MEAL; ENDOTHELIAL FUNCTION; VASCULAR FUNCTION; EPOXYEICOSATRIENOIC ACIDS; CROSSOVER TRIAL; NUT CONSUMPTION; DISEASE AB Improved vascular function after the incorporation of walnuts into controlled or high-fat diets has been reported; however, the mechanism(s) underlying this effect of walnuts is(are) poorly defined. The objective of the current study was to evaluate the acute and short-term effects of walnut intake on changes in microvascular function and the relationship of these effects to plasma epoxides, the cytochrome-P450-derived metabolites of fatty acids. Thirty-eight hypercholesterolemic postmenopausal women were randomized to 4 weeks of 5 g or 40 g of daily walnut intake. All outcomes were measured after an overnight fast and 4 h after walnut intake. Microvascular function, assessed as the reactive hyperemia index (RHI), was the primary outcome measure, with serum lipids and plasma epoxides as secondary measures. Compared to 5 g of daily walnut intake, consuming 40 g/d of walnuts for 4 weeks increased the RHI and Framingham RHI. Total cholesterol and low- and high-density cholesterol did not significantly change after walnut intake. The change in RHI after 4 weeks of walnut intake was associated with the change in the sum of plasma epoxides (r=0.65, P=.002) but not with the change in the sum of plasma hydroxyeicosatetraenoic acids. Of the individual plasma epoxides, arachidonic-acid-derived 14(15)-epoxyeicosatrienoic acid was most strongly associated with the change in microvascular function (r=0.72, P<.001). These data support the concept that the intake of walnut-derived fatty acids can favorably affect plasma epoxide production, resulting in improved microvascular function. (C) 2015 Elsevier Inc. All rights reserved. C1 [Holt, Roberta R.; Yim, Sun J.; Hackman, Robert M.; Djurica, Dragana; Newman, John W.; Keen, Carl L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Keen, Carl L.] Univ Calif, Davis Med Ctr, Dept Internal Med, Sacramento, CA 95817 USA. [Shindel, Alan W.] Univ Calif, Dept Urol, Davis Med Ctr, Sacramento, CA 95817 USA. [Shearer, Gregory C.] Sanford Res USD, Cardiovasc Hlth Res Ctr, Sioux Falls, SD 57104 USA. [Shearer, Gregory C.] Univ S Dakota, Sanford Sch Med, Dept Internal Med, Sioux Falls, SD 57105 USA. [Shearer, Gregory C.] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. [Newman, John W.] USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Holt, RR (reprint author), Univ Calif Davis, Dept Nutr, One Shields Ave, Davis, CA 95616 USA. EM rrholt@ucdavis.edu FU California Walnut Commission; USDA Intramural Project [5306-51530-019-00D]; USDA NIFA National Needs Graduate Fellowship; Agriculture and Food Research Initiative Competitive Grant [2012-01370] FX Research funding provided in part by the California Walnut Commission. Partial support was also provided by USDA Intramural Project 5306-51530-019-00D to J.W. Newman, by USDA NIFA National Needs Graduate Fellowship to D. Djurica and by the Agriculture and Food Research Initiative Competitive Grant #2012-01370 to R.R. Holt. Other than the funding source, all authors report no disclosures relevant to the content of this paper. NR 51 TC 0 Z9 0 U1 0 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD DEC PY 2015 VL 26 IS 12 BP 1458 EP 1466 DI 10.1016/j.jnutbio.2015.07.012 PG 9 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CZ0HL UT WOS:000366785600007 PM 26396054 ER PT J AU Davis, TJ Kaufman, PE Hogsette, JA Kline, DL AF Davis, Timothy J. Kaufman, Phillip E. Hogsette, Jerome A. Kline, Daniel L. TI THE EFFECTS OF LARVAL HABITAT QUALITY ON AEDES ALBOPICTUS SKIP OVIPOSITION SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Asian tiger mosquito; container habitat; installment oviposition; mosquito control; mosquito management ID AEGYPTI DIPTERA; TRISERIATUS DIPTERA; FIELD-EVALUATION; LETHAL OVITRAP; CULICIDAE; MOSQUITO; EGGS; DISPERSAL; INFUSIONS; THAILAND AB Aedes albopictus is an invasive mosquito species that transmits human-disease-causing pathogens. It is a container-inhabiting species that oviposits in resource-limited habitats. To mitigate larval competition, Ae. albopictus females may choose to distribute eggs from a single gonotrophic cycle among multiple containers through skip oviposition. With the use of individual females released in indoor and outdoor caged trials, we evaluated the oviposition choices made by gravid Ae. albopictus offered larval habitats with different qualities. Our results demonstrate that Ae. albopictus performs skip oviposition and that the degree of egg distribution is related to the quality of the larval habitat. In a 4-choice arena, individual Ae. albopictus oviposited in fewer containers when presented with ovisites of high-quality larval habitat (uncrowded conditions) compared with oviposition in low-quality (crowded conditions) larval habitats. Additionally, the females selectively oviposited in high-quality habitats when offered both low-and high-quality habitats, but distributed eggs more evenly among multiple high-quality habitats. Our results have important implications for mosquito management plans that include the use of lethal ovitraps, as well as the role of this behavior in distribution of disease-causing pathogens. C1 [Davis, Timothy J.; Kaufman, Phillip E.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Hogsette, Jerome A.; Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Kaufman, PE (reprint author), Univ Florida, Dept Entomol & Nematol, POB 110620, Gainesville, FL 32611 USA. NR 39 TC 2 Z9 2 U1 4 U2 17 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD DEC PY 2015 VL 31 IS 4 BP 321 EP 328 PG 8 WC Entomology SC Entomology GA CZ5VL UT WOS:000367170300003 PM 26675453 ER PT J AU Reed, S Neuman, H Moscovich, S Glahn, RP Koren, O Tako, E AF Reed, Spenser Neuman, Hadar Moscovich, Sharon Glahn, Raymond P. Koren, Omry Tako, Elad TI Chronic Zinc Deficiency Alters Chick Gut Microbiota Composition and Function SO NUTRIENTS LA English DT Article DE zinc deficiency; microbiota; dysbiosis; cecal microbiome; zinc biomarker ID HIGH DIETARY ZINC; CHAIN FATTY-ACIDS; BROILER-CHICKENS; WEANED PIGLETS; GALLUS-GALLUS; INTESTINAL MICROBIOTA; BACTERIAL COMMUNITIES; DEVELOPING-COUNTRIES; ESCHERICHIA-COLI; RESISTANT STARCH AB Zinc (Zn) deficiency is a prevalent micronutrient insufficiency. Although the gut is a vital organ for Zn utilization, and Zn deficiency is associated with impaired intestinal permeability and a global decrease in gastrointestinal health, alterations in the gut microbial ecology of the host under conditions of Zn deficiency have yet to be studied. Using the broiler chicken (Gallus gallus) model, the aim of this study was to characterize distinct cecal microbiota shifts induced by chronic dietary Zn depletion. We demonstrate that Zn deficiency induces significant taxonomic alterations and decreases overall species richness and diversity, establishing a microbial profile resembling that of various other pathological states. Through metagenomic analysis, we show that predicted Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways responsible for macro- and micronutrient uptake are significantly depleted under Zn deficiency; along with concomitant decreases in beneficial short chain fatty acids, such depletions may further preclude optimal host Zn availability. We also identify several candidate microbes that may play a significant role in modulating the bioavailability and utilization of dietary Zn during prolonged deficiency. Our results are the first to characterize a unique and dysbiotic cecal microbiota during Zn deficiency, and provide evidence for such microbial perturbations as potential effectors of the Zn deficient phenotype. C1 [Reed, Spenser; Glahn, Raymond P.; Tako, Elad] USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Reed, Spenser] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Reed, Spenser] Univ Arizona, Coll Med, Tucson, AZ 85724 USA. [Neuman, Hadar; Moscovich, Sharon; Koren, Omry] Bar Ilan Univ, Fac Med, IL-1311502 Safed, Israel. RP Tako, E (reprint author), USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM smr2@cornell.edu; hadarneuman@gmail.com; sharonmosc@gmail.com; rpg3@cornell.edu; Omry.Koren@biu.ac.il; et79@cornell.edu OI Koren, Omry/0000-0002-7738-1337 FU NIDDK NIH HHS [U24 DK097153] NR 94 TC 7 Z9 8 U1 7 U2 18 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD DEC PY 2015 VL 7 IS 12 BP 9768 EP 9784 DI 10.3390/nu7125497 PG 17 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CZ4DA UT WOS:000367052200005 PM 26633470 ER PT J AU Blumberg, JB Vita, JA Chen, CYO AF Blumberg, Jeffrey B. Vita, Joseph A. Chen, C. -Y. Oliver TI Concord Grape Juice Polyphenols and Cardiovascular Risk Factors: Dose-Response Relationships SO NUTRIENTS LA English DT Article DE concord grape juice; polyphenols; flavonoids; cardiovascular risk factors; blood pressure; platelet aggregation; flow-mediated dilation; LDL oxidation ID IMPROVES ENDOTHELIAL FUNCTION; CORONARY-ARTERY-DISEASE; RANDOMIZED CONTROLLED-TRIAL; LOW-DENSITY-LIPOPROTEIN; FLOW-MEDIATED DILATION; AMBULATORY BLOOD-PRESSURE; 100-PERCENT FRUIT JUICE; DARK CHOCOLATE; FLAVONOID INTAKE; PLATELET-AGGREGATION AB Pure fruit juices provide nutritional value with evidence suggesting some of their benefits on biomarkers of cardiovascular disease risk may be derived from their constituent polyphenols, particularly flavonoids. However, few data from clinical trials are available on the dose-response relationship of fruit juice flavonoids to these outcomes. Utilizing the results of clinical trials testing single doses, we have analyzed data from studies of 100% Concord grape juice by placing its flavonoid content in the context of results from randomized clinical trials of other polyphenol-rich foods and beverages describing the same outcomes but covering a broader range of intake. We selected established biomarkers determined by similar methods for measuring flow-mediated vasodilation (FMD), blood pressure, platelet aggregation, and the resistance of low density lipoprotein cholesterol (LDL) to oxidation. Despite differences among the clinical trials in the treatment, subjects, and duration, correlations were observed between the dose and FMD. Inverse dose-response relationships, albeit with lower correlation coefficients, were also noted for the other outcomes. These results suggest a clear relationship between consumption of even modest serving sizes of Concord grape juice, flavonoid intake, and effects on risk factors for cardiovascular disease. This approach to dose-response relationships may prove useful for testing other individual foods and beverages. C1 [Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Antioxidants Res Lab, Boston, MA 02111 USA. [Vita, Joseph A.] Boston Univ, Sch Med, Evans Dept Med, Boston, MA 02118 USA. [Vita, Joseph A.] Boston Univ, Sch Med, Whitaker Cardiovasc Inst, Boston, MA 02118 USA. RP Blumberg, JB (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Antioxidants Res Lab, Boston, MA 02111 USA. EM jeffrey.blumberg@tufts.edu; jvita@bu.edu; oliver.chen@tufts.edu OI Blumberg, Jeffrey/0000-0003-3871-8635 FU Welch Foods, Inc. FX This work was carried out through the USDA Agricultural Research Service under Cooperative Agreement No. 58-1950-014. Welch Foods, Inc. offered support for this article by providing an honorarium to Jeffrey B. Blumberg and C-Y. Oliver Chen. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 99 TC 4 Z9 4 U1 3 U2 13 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD DEC PY 2015 VL 7 IS 12 BP 10032 EP 10052 DI 10.3390/nu7125519 PG 21 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CZ4DA UT WOS:000367052200023 PM 26633488 ER PT J AU Thangthaeng, N Miller, MG Gomes, SM Shukitt-Hale, B AF Thangthaeng, Nopporn Miller, Marshall G. Gomes, Stacey M. Shukitt-Hale, Barbara TI Daily supplementation with mushroom (Agaricus bisporus) improves balance and working memory in aged rats SO NUTRITION RESEARCH LA English DT Article DE Agaricus bisporus; White button mushroom; Diet; Aging; Memory; Balance ID VITAMIN-D; PSYCHOMOTOR; PERFORMANCE; COGNITION; IMPAIRMENT; BEHAVIOR; CELLS AB Decline in brain function during normal aging is partly due to the long-term effects of oxidative stress and inflammation. Several fruits and vegetables have been shown to possess antioxidant and anti-inflammatory properties. The present study investigated the effects of dietary mushroom intervention on mobility and memory in aged Fischer 344 rats. We hypothesized that daily supplementation of mushroom would have beneficial effects on behavioral outcomes in a dose-dependent manner. Rats were randomly assigned to receive a diet containing either 0%, 0.5%, 1%, 2%, or 5% lyophilized white button mushroom (Agaricus bisporus); after 8 weeks on the diet, a battery of behavioral tasks was given to assess balance, coordination, and cognition. Rats on the 2% or 5% mushroom-supplemented diet consumed more food, without gaining weight, than rats in the other diet groups. Rats in the 0.5% and 1% group stayed on a narrow beam longer, indicating an improvement in balance. Only rats on the 0.5% mushroom diet showed improved performance in a working memory version of the Morris water maze. When taken together, the most effective mushroom dose that produced improvements in both balance and working memory was 0.5%, equivalent to about 1.5 ounces of fresh mushrooms for humans. Therefore, the results suggest that the inclusion of mushroom in the daily diet may have beneficial effects on age-related deficits in cognitive and motor function. Published by Elsevier Inc. C1 [Thangthaeng, Nopporn; Miller, Marshall G.; Gomes, Stacey M.; Shukitt-Hale, Barbara] Tufts Univ, USDA ARS, HNRCA, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA ARS, HNRCA, 711 Washington St, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov FU USDA intramural funds; Mushroom Council (San Jose, CA, USA) FX This work was supported by USDA intramural funds and the Mushroom Council (San Jose, CA, USA). The authors would like to acknowledge the contributions of the late James A. Joseph, who initiated the work on this project, and the helpful advice of Mary Jo Feeney throughout the project. NR 28 TC 0 Z9 2 U1 4 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD DEC PY 2015 VL 35 IS 12 BP 1079 EP 1084 DI 10.1016/j.nutres.2015.09.012 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CZ1RQ UT WOS:000366883400006 PM 26475179 ER PT J AU Shen, CL Han, J Wang, S Chung, EH Chyu, MC Cao, JJ AF Shen, Chwan-Li Han, Jia Wang, Shu Chung, Eunhee Chyu, Ming-Chien Cao, Jay J. TI Green tea supplementation benefits body composition and improves bone properties in obese female rats fed with high-fat diet and caloric restricted diet SO NUTRITION RESEARCH LA English DT Article DE Tea; Obesity; Caloric restriction; Body composition; Osteoporosis; Rat ID BROWN ADIPOSE-TISSUE; ACTIVATED-RECEPTOR-GAMMA; MINERAL DENSITY; CORTICAL BONE; MECHANICAL-PROPERTIES; METABOLIC SYNDROME; ANOREXIA-NERVOSA; YOUNG-WOMEN; WEIGHT-LOSS; MARROW FAT AB This study investigated the effects of green tea polyphenols (GTP) supplementation on body composition, bone properties, and serum markers in obese rats fed a high-fat diet (HFD) or a caloric restricted diet (CRD). Forty-eight female rats were fed an HFD ad libitum for 4 months, and then either continued on the HFD or the CRD with or without 0.5% GTP in water. Body composition, bone efficacy, and serum markers were measured. We hypothesized that GTP supplementation would improve body composition, mitigate bone loss, and restore bone microstructure in obese animals fed either HFD or CRD. CRD lowered percent fat mass; bone mass and trabecular number of tibia, femur and lumbar vertebrae; femoral strength; trabecular and cortical thickness of tibia; insulin-like growth factor-I and leptin. CRD also increased percent fat-free mass; trabecular separation of tibia and femur; eroded surface of tibia; bone formation rate and erosion rate at tibia shaft; and adiponectin. GTP supplementation increased femoral mass and strength (P =.026), trabecular thickness (P =.012) and number (P =.019), and cortical thickness of tibia (P <.001), and decreased trabecular separation (P =.021), formation rate (P <.001), and eroded surface (P <.001) at proximal tibia, and insulin-like growth factor-I and leptin. There were significant interactions (diet type x GTP) on osteoblast surface/bone surface, mineral apposition rate at periosteal and endocortical bones, periosteal bone formation rate, and trabecular thickness at femur and lumbar vertebrate (P <.05). This study demonstrates that GTP supplementation for 4 months benefited body composition and improved bone microstructure and strength in obese rats fed with HFD or HFD followed by CRD diet. (C) 2015 Elsevier Inc. All rights reserved. C1 [Shen, Chwan-Li; Han, Jia; Chyu, Ming-Chien] Texas Tech Univ, Hlth Sci Ctr, Dept Pathol, Lubbock, TX 79430 USA. [Shen, Chwan-Li] Texas Tech Univ, Hlth Sci Ctr, Laura W Bush Inst Womens Hlth, Lubbock, TX 79430 USA. [Wang, Shu] Texas Tech Univ, Dept Nutr Sci, Lubbock, TX 79430 USA. [Chung, Eunhee] Texas Tech Univ, Hlth Exercise & Sport Sci, Lubbock, TX 79430 USA. [Chyu, Ming-Chien] Texas Tech Univ, Healthcare Engn Grad Program, Lubbock, TX 79430 USA. [Cao, Jay J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Shen, CL (reprint author), Texas Tech Univ, Hlth Sci Ctr, Dept Pathol, 1A092,3601 4th St, Lubbock, TX 79430 USA. EM leslie.shen@ttuhsc.edu OI Chung, Eunhee/0000-0002-4501-9333 FU Laura W. Bush Institute for Women's Health; USDA ARS program "Food Factors to Prevent Obesity and Related Diseases", CRIS [5450-51000-048-00D] FX This study was supported by Laura W. Bush Institute for Women's Health to CLS and the USDA ARS program "Food Factors to Prevent Obesity and Related Diseases", CRIS no. 5450-51000-048-00D to JJC. NR 73 TC 1 Z9 1 U1 5 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD DEC PY 2015 VL 35 IS 12 BP 1095 EP 1105 DI 10.1016/j.nutres.2015.09.014 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CZ1RQ UT WOS:000366883400008 PM 26525915 ER PT J AU Hu, XP Gurung, S Short, DPG Sandoya, GV Shang, WJ Hayes, RJ Davis, RM Subbarao, KV AF Hu, Xiao-Ping Gurung, Suraj Short, Dylan P. G. Sandoya, German V. Shang, Wen-Jing Hayes, Ryan J. Davis, R. Michael Subbarao, Krishna V. TI Nondefoliating and Defoliating Strains from Cotton Correlate with Races 1 and 2 of Verticillium dahliae SO PLANT DISEASE LA English DT Article ID VEGETATIVE COMPATIBILITY; MOLECULAR VARIATION; RESISTANCE GENE; GOSSYPIUM-HIRSUTUM; INOCULUM DENSITY; ALBO-ATRUM; TOMATO VE1; WILT; PATHOGENICITY; DIFFERENTIATION AB Verticillium wilt, caused by Verticillium dahliae, is an important disease of cotton worldwide. Isolates of V. dahliae can be characterized as race 1 or race 2 based on the responses of differential cultivars of tomato and lettuce, or as defoliating or nondefoliating based on symptom expression in cotton. To investigate the frequency and distribution of races and defoliation phenotypes of cotton-associated V. dahliae, 317 isolates from China, Israel, Turkey, and the United States were tested by polymerase chain reaction (PCR) using defoliating, nondefoliating, and race 1- and race 2-specific primers DF/DR, NDF/NDR, VdAve1F/VdAve1R, and VdR2F/VdR2R, respectively. Of the total, 97.2% of isolates genotyped as defoliating were also characterized as race 2, while 90.8% of isolates genotyped as nondefoliating were also genotyped as race 1. To verify these results, three cotton cultivars -'FM 2484B2F' (highly resistant), '98M-2983' (highly susceptible), and 'CA4002' (partially resistant)-used as differentials were each inoculated with 10 isolates characterized by PCR: six defoliating/race 2 strains (GH1005, GH1021, FIN, XJ2008, XJ592, and reference strain Ls17) and four nondefoliating/race 1 strains (GH1015, GH1016, GH1020, and reference strain Ls16). All defoliating/race 2 isolates except for Ls17 caused defoliation on 98M-2983 and CA4002. Isolate Ls17 caused defoliation on 98M-2983 only. The nondefoliating/race 1 isolates caused Verticillium wilt symptoms devoid of defoliation on 98M-2983. The greenhouse assays confirmed the molecular identification of race and defoliation phenotype. Although the existence of races has not been previously established among V. dahliae isolates from cotton, the long-established nondefoliating and defoliating population structure corresponded with V. dahliae races 1 and 2, respectively. C1 [Hu, Xiao-Ping; Shang, Wen-Jing] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China. [Hu, Xiao-Ping; Shang, Wen-Jing] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Peoples R China. [Gurung, Suraj; Short, Dylan P. G.; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, Y Salinas, CA 93905 USA. [Sandoya, German V.] Univ Calif Davis, Genome Ctr, Salinas, CA 93905 USA. [Sandoya, German V.] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Hayes, Ryan J.] ARS, USDA, Salinas, CA 93905 USA. [Davis, R. Michael] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, Y Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU NSFC [31371888]; 111 project from Education Ministry of China [B07049]; Hatch Funds FX The study was supported partially by NSFC (31371888) and the 111 project from Education Ministry of China (number B07049) and Hatch Funds allocated to the University of California, Davis. We thank H. Q. Zhu for providing isolates for this study and T A. Wheeler for providing cotton cultivars evaluated in this study. NR 51 TC 3 Z9 3 U1 1 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2015 VL 99 IS 12 BP 1713 EP 1720 DI 10.1094/PDIS-03-15-0261-RE PG 8 WC Plant Sciences SC Plant Sciences GA CZ0GJ UT WOS:000366782800006 ER PT J AU O'Keefe, G Davis, DD AF O'Keefe, G. Davis, D. D. TI Morphology of Puccinia horiana, Causal Agent of Chrysanthemum White Rust, Sampled From Naturally Infected Plants SO PLANT DISEASE LA English DT Article ID VASCULAR INFECTION; SYSTEMIC INFECTION; X MORIFOLIUM; GERMINATION; FUNGI; PENNSYLVANIA; VULGARIS AB Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum x morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur. C1 [Davis, D. D.] Penn State Univ, USDA, Anim & Plant Hlth Inspect Serv Plant Protect & Qu, University Pk, PA 16802 USA. Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Davis, DD (reprint author), Penn State Univ, USDA, Anim & Plant Hlth Inspect Serv Plant Protect & Qu, University Pk, PA 16802 USA. EM ddd2@psu.edu FU Pennsylvania State University, College of Agricultural Sciences, Department of Plant Pathology and Environmental Microbiology FX This research was made possible by funding from The Pennsylvania State University, College of Agricultural Sciences, Department of Plant Pathology and Environmental Microbiology. We thank M. Hazen and J. Cantolina of the Microscopy and Cytometry Facility, Huck Institute of the Life Sciences, The Pennsylvania State University for their SEM technical expertise. NR 32 TC 0 Z9 0 U1 9 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2015 VL 99 IS 12 BP 1738 EP 1743 DI 10.1094/PDIS-02-15-0239-RE PG 6 WC Plant Sciences SC Plant Sciences GA CZ0GJ UT WOS:000366782800010 ER PT J AU Moore, MJ Ostry, ME AF Moore, M. J. Ostry, M. E. TI Influence of Temperature and Humidity on the Viability of Ophiognomonia clavigignenti-juglandacearum Conidia SO PLANT DISEASE LA English DT Article ID BUTTERNUT CANKER FUNGUS; GERMINATION; LONGEVITY; SURVIVAL; EXTRACTS; TREES AB Butternut canker, caused by the fungus Ophiognomonia clavigignenti-juglandacearum, primarily kills butternut (Juglans cinerea). Rain splash and local air currents are the primary means of conidia dispersal but that does not explain its long-distance spread and infection of isolated trees. Dispersal by insect or animal vectors or plant material likely necessitates the ability for conidia to tolerate drying for a period of time over variable temperature and humidity conditions. The objective of this study was to determine the influence of temperature and humidity on conidial germination and survival of air-dried conidia. Conidia collected from 1-month-old cultures germinated on water agar over a wide range of temperatures (4 to 32 degrees C) and were viable after brief periods at 36 degrees C when returned to a lower temperature. Viability of air-dried conidia held on nylon membranes at various temperatures and humidities varied from less than a day at 28 degrees C and 90% relative humidity (RH) to a mean of 15 days at 20 degrees C and 80% RH. RH had the least effect on viability at 12 degrees C, with conidia remaining viable for 7 days at most humidity levels tested. Conidia held at 100% RH began germinating on the membranes after 21 days. Conidia in a water suspension remained viable for 168 days at all temperatures tested. These results suggest that O. clavigignenti-juglandacearum conidia may remain viable on the surface of a vector or plant material and seed for over 2 weeks, given the proper conditions, or for much longer if in water or in an environment of saturated humidity. This potential may, in part, explain the frequent presence of the disease on isolated trees. C1 [Moore, M. J.; Ostry, M. E.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. RP Moore, MJ (reprint author), US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. EM melaniemoore@fs.fed.us NR 27 TC 0 Z9 0 U1 2 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2015 VL 99 IS 12 BP 1841 EP 1846 DI 10.1094/PDIS-09-14-0976-RE PG 6 WC Plant Sciences SC Plant Sciences GA CZ0GJ UT WOS:000366782800023 ER PT J AU Machado, FJ Moller, PA Nicolli, CP Del Ponte, EM Ward, TJ AF Machado, F. J. Moeller, P. A. Nicolli, C. P. Del Ponte, E. M. Ward, T. J. TI First Report of Fusarium graminearum, F. asiaticum, and F. cortaderiae as Head Blight Pathogens of Annual Ryegrass in Brazil SO PLANT DISEASE LA English DT News Item C1 [Machado, F. J.; Moeller, P. A.; Nicolli, C. P.; Del Ponte, E. M.] Univ Fed Vicosa, Dept Fitopatol, BR-36570000 Vicosa, MG, Brazil. [Ward, T. J.] USDA ARS, Peoria, IL USA. RP Machado, FJ (reprint author), Univ Fed Vicosa, Dept Fitopatol, BR-36570000 Vicosa, MG, Brazil. NR 5 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2015 VL 99 IS 12 BP 1859 EP 1859 DI 10.1094/PDIS-04-15-0376-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CZ0GJ UT WOS:000366782800040 ER PT J AU Browne, GT Schmidt, LS Brar, G AF Browne, G. T. Schmidt, L. S. Brar, G. TI First Report of Phytophthora niederhauserii Causing Crown Rot of Almond (Prunus dulcis) in California SO PLANT DISEASE LA English DT News Item ID TREES C1 [Browne, G. T.; Schmidt, L. S.] Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, Dept Plant Pathol, Davis, CA 95616 USA. [Brar, G.] Univ Calif Cooperat Extens, Fresno, CA 93710 USA. RP Browne, GT (reprint author), Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, Dept Plant Pathol, Davis, CA 95616 USA. NR 3 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2015 VL 99 IS 12 BP 1863 EP 1863 DI 10.1094/PDIS-09-14-0995-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CZ0GJ UT WOS:000366782800050 ER PT J AU Bjork, KE Kopral, CA Wagner, BA Dargatz, DA AF Bjork, K. E. Kopral, C. A. Wagner, B. A. Dargatz, D. A. TI Comparison of mixed effects models of antimicrobial resistance metrics of livestock and poultry Salmonella isolates from a national monitoring system SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Antimicrobial resistance; Monitoring; Livestock; Mixed effects modeling; Multidrug resistance AB Antimicrobial use in agriculture is considered a pathway for the selection and dissemination of resistance determinants among animal and human populations. From 1997 through 2003 the U.S. National Antimicrobial Resistance Monitoring System (NARMS) tested clinical Salmonella isolates from multiple animal and environmental sources throughout the United States for resistance to panels of 16-19 antimicrobials. In this study we applied two mixed effects models, the generalized linear mixed model (GLMM) and accelerated failure time frailty (AFT-frailty) model, to susceptible/resistant and interval-censored minimum inhibitory concentration (MIC) metrics, respectively, from Salmonella enterica subspecies enterica serovar Typhimurium isolates from livestock and poultry. Objectives were to compare characteristics of the two models and to examine the effects of time, species, and multidrug resistance (MDR) on the resistance of isolates to individual antimicrobials, as revealed by the models. Fixed effects were year of sample collection, isolate source species and MDR indicators; laboratory study site was included as a random effect. MDR indicators were significant for every antimicrobial and were dominant effects in multivariable models. Temporal trends and source species influences varied by antimicrobial. In GLMMs, the intra-class correlation coefficient ranged up to 0.8, indicating that the proportion of variance accounted for by laboratory study site could be high. AFT models tended to be more sensitive, detecting more curvilinear temporal trends and species differences; however, high levels of left- or right-censoring made some models unstable and results uninterpretable. Results from GLMMs may be biased by cutoff criteria used to collapse MIC data into binary categories, and may miss signaling important trends or shifts if the series of antibiotic dilutions tested does not span a resistance threshold. Our findings demonstrate the challenges of measuring the AMR ecosystem and the complexity of interacting factors, and have implications for future monitoring. We include suggestions for future data collection and analyses, including alternative modeling approaches. Published by Elsevier B.V. C1 [Bjork, K. E.; Kopral, C. A.; Wagner, B. A.; Dargatz, D. A.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. RP Bjork, KE (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B,MS 2E7, Ft Collins, CO 80526 USA. EM Kathe.e.bjork@aphis.usda.gov NR 30 TC 2 Z9 2 U1 2 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD DEC 1 PY 2015 VL 122 IS 3 BP 265 EP 272 DI 10.1016/j.prevetmed.2015.10.010 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CZ0FJ UT WOS:000366780200003 PM 26597093 ER PT J AU Coudron, TA Meeds, A Bailey, C Meihls, LN AF Coudron, Thomas A. Meeds, Andrew Bailey, Cornell Meihls, Lisa N. TI Viable Progeny from Crosses between Geographically Isolated Populations of Podisus (Say)(1) Indicate a Single Species SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID SPINED SOLDIER BUG; MACULIVENTRIS HEMIPTERA; PENTATOMIDAE; HETEROPTERA; FIELD AB Discrepancies in reports on the presence of spined soldier bug, Podisus maculiventris (Say) (Hemiptera: Pentatomidae), in the western region of the USA, and morphological variations of the species brought into question whether the species existed west of the Rocky Mountains. In this study, morphological variations in color and size were observed between two geographically isolated populations from Missouri and California. Differences were determined in color, weight, and size measurements. However, all crosses and backcrosses produced viable progeny of both genders and the crosses were maintained as inbred lines for 16 generations. These results indicated the two populations are closely related. C1 [Coudron, Thomas A.] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Meeds, Andrew] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Bailey, Cornell] Lincoln Univ, Jefferson City, MO 65101 USA. [Meihls, Lisa N.] Univ Missouri, USDA, ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Coudron, TA (reprint author), ARS, USDA, Biol Control Insects Res Lab, 1503 South Providence Rd,Res Pk, Columbia, MO 65203 USA. EM tom.coudron@ars.usda.gov NR 17 TC 0 Z9 0 U1 0 U2 0 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD DEC PY 2015 VL 40 IS 4 BP 677 EP 689 PG 13 WC Entomology SC Entomology GA CY8SS UT WOS:000366679200001 ER PT J AU Cheng, LW Henderson, TD Lam, TI Stanker, LH AF Cheng, Luisa W. Henderson, Thomas D., II Lam, Tina I. Stanker, Larry H. TI Use of Monoclonal Antibodies in the Sensitive Detection and Neutralization of Botulinum Neurotoxin Serotype B SO TOXINS LA English DT Article DE monoclonal antibodies; mouse models; botulinum neurotoxins; toxin neutralization; toxicokinetics ID LINKED-IMMUNOSORBENT-ASSAY; DOMAIN-BASED ASSAYS; MOUSE BIOASSAY; TOXIN; FOOD; SUBUNIT; VACCINE; PROTEIN; SAMPLES; ELISA AB Botulinum neurotoxins (BoNT) are some of nature's most potent toxins. Due to potential food contamination, and bioterrorism concerns, the development of detection reagents, therapeutics and countermeasures are of urgent interest. Recently, we have developed a sensitive electrochemiluminescent (ECL) immunoassay for BoNT/B, using monoclonal antibodies (mAbs) MCS6-27 and anti-BoNT/B rabbit polyclonal antibodies as the capture and detector. The ECL assay detected as little as 1 pg/mL BoNT/B in the buffer matrix, surpassing the detection sensitivities of the gold standard mouse bioassays. The ECL assay also allowed detection of BoNT/B in sera matrices of up to 100% sera with negligible matrix effects. This highly-sensitive assay allowed the determination of the biological half-lives of BoNT/B holotoxin in vivo. We further tested the toxin neutralization potential of our monoclonal antibodies using the mouse systemic and oral intoxication models. A combination of mAbs protected mice in both pre- and post-exposure models to lethal doses of BoNT/B. MAbs were capable of increasing survival of animals when administered even 10 h post-intoxication in an oral model, suggesting a likely time for BoNT/B complexes to reach the blood stream. More sensitive detection assays and treatments against BoNT intoxication will greatly enhance efforts to combat botulism. C1 [Cheng, Luisa W.; Henderson, Thomas D., II; Stanker, Larry H.] ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Lam, Tina I.] Gilead Sci Inc, Foster City, CA 94404 USA. RP Cheng, LW (reprint author), ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM luisa.cheng@ars.usda.gov; thomas.henderson@ars.usda.gov; tinaiunsanlam@gmail.com; larry.stanker@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service, National Program [NP108, CRIS 5325-42000-048-00D]; National Institute of Allergy And Infectious Diseases Service [U54 AI065359]; Department of Homeland Security [40768] FX The authors would like to acknowledge Christina Tam, Reuven Rassooly and Wallace Yokoyama for critical review of this manuscript; Wanless Hatcher and Zeke Martinez for their help with animal care and handling. This work was funded by the United States Department of Agriculture, Agricultural Research Service, National Program project NP108, CRIS 5325-42000-048-00D. L.W.C. was also funded by the National Institute of Allergy And Infectious Diseases Service Grant U54 AI065359 and L.H.S. was also funded by interagency agreement IAA#40768 with the Department of Homeland Security. NR 36 TC 1 Z9 1 U1 5 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD DEC PY 2015 VL 7 IS 12 BP 5068 EP 5078 DI 10.3390/toxins7124863 PG 11 WC Toxicology SC Toxicology GA CZ3OI UT WOS:000367013500012 PM 26633496 ER PT J AU Zhu, Y Ye, XH Liu, Y Yan, ZC Stanley, D Ye, GY Fang, Q AF Zhu, Yu Ye, Xin-Hai Liu, Yang Yan, Zhi-Chao Stanley, David Ye, Gong-Yin Fang, Qi TI A Venom Gland Extracellular Chitin-Binding-Like Protein from Pupal Endoparasitoid Wasps, Pteromalus Puparum, Selectively Binds Chitin SO TOXINS LA English DT Article DE parasitoid; chitin binding protein; venom apparatus; venom proteins ID PERITROPHIC MATRIX PROTEIN; PIERIS-RAPAE LEPIDOPTERA; CUTICULAR PROTEINS; NASONIA-VITRIPENNIS; SERRATIA-MARCESCENS; ANOPHELES-GAMBIAE; GENE-EXPRESSION; HYMENOPTERA; HOST; DOMAIN AB Chitin-binding proteins (CBPs) are present in many species and they act in a variety of biological processes. We analyzed a Pteromalus puparum venom apparatus proteome and transcriptome and identified a partial gene encoding a possible CBP. Here, we report cloning a full-length cDNA of a sequence encoding a chitin-binding-like protein (PpCBP) from P. puparum, a pupal endoparasitoid of Pieris rapae. The cDNA encoded a 96-amino-acid protein, including a secretory signal peptide and a chitin-binding peritrophin-A domain. Phylogenetic analysis of chitin binding domains (CBDs) of cuticle proteins and peritrophic matrix proteins in selected insects revealed that the CBD of PpCBP clustered with the CBD of Nasonia vitripennis. The PpCBP is specifically expressed in the venom apparatus of P. puparum, mostly in the venom gland. PpCBP expression was highest at day one after adult eclosion and much lower for the following five days. We produced a recombinant PpCBP and binding assays showed the recombinant protein selectively binds chitin but not cellulose in vitro. We infer that PpCBP serves a structural role in the venom reservoir, or may be injected into the host to help wound healing of the host exoskeleton. C1 [Zhu, Yu; Liu, Yang; Yan, Zhi-Chao; Ye, Gong-Yin; Fang, Qi] Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China. [Zhu, Yu; Liu, Yang; Yan, Zhi-Chao; Ye, Gong-Yin; Fang, Qi] Zhejiang Univ, Inst Insect Sci, Key Lab Agr Entomol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China. [Ye, Xin-Hai] South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Fang, Q (reprint author), Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China. EM yuzhu@zju.edu.cn; yexinhai1204@hotmail.com; sammie90@126.com; yan_zc@126.com; stanleyd@missouri.edu; chu@zju.edu.cn; fangqi@zju.edu.cn FU National Program on Key Basic Research Projects (973 Program) [2013CB127600]; National Nature Science Foundation of China [31272098]; Research Fund for the Doctoral Program of Higher Education of China [2012010113004]; Zhejiang Provincial Natural Science Foundation of China [Y14C140006]; Fundamental Research Funds for the Central Universities [2014FZA6014] FX This study was supported by National Program on Key Basic Research Projects (973 Program, 2013CB127600), National Nature Science Foundation of China (Grant number: 31272098), Research Fund for the Doctoral Program of Higher Education of China (Grant number: 2012010113004), Zhejiang Provincial Natural Science Foundation of China (Grant number: Y14C140006), and the Fundamental Research Funds for the Central Universities (Grant number: 2014FZA6014). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 41 TC 0 Z9 0 U1 6 U2 11 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD DEC PY 2015 VL 7 IS 12 BP 5098 EP 5113 DI 10.3390/toxins7124867 PG 16 WC Toxicology SC Toxicology GA CZ3OI UT WOS:000367013500016 PM 26633500 ER PT J AU Silva, CJ Erickson-Beltran, ML Skinner, CB Patfield, SA He, XH AF Silva, Christopher J. Erickson-Beltran, Melissa L. Skinner, Craig B. Patfield, Stephanie A. He, Xiaohua TI Mass Spectrometry-Based Method of Detecting and Distinguishing Type 1 and Type 2 Shiga-Like Toxins in Human Serum SO TOXINS LA English DT Article DE Keywords: mass spectrometry; Shiga toxins; verotoxins; multiple reaction monitoring method (MRM); STEC; serum; shigatoxigenic and enterohemorrhagic strains of Escherichia coli; EHEC; Enterobacter ID HEMOLYTIC-UREMIC SYNDROME; AMYLOID-P COMPONENT; ENTEROHEMORRHAGIC ESCHERICHIA-COLI; ENTEROBACTER-CLOACAE; ATTOMOLE AMOUNTS; PRION PROTEIN; IDENTIFICATION; BACTERIOPHAGE; PATHOGENESIS; STABILITY AB Shiga-like toxins (verotoxins) are responsible for the virulence associated with a variety of foodborne bacterial pathogens. Direct detection of toxins requires a specific and sensitive technique. In this study, we describe a mass spectrometry-based method of analyzing the tryptic decapeptides derived from the non-toxic B subunits. A gene encoding a single protein that yields a set of relevant peptides upon digestion with trypsin was designed. The N-15-labeled protein was prepared by growing the expressing bacteria in minimal medium supplemented with (NH4Cl)-N-15. Trypsin digestion of the N-15-labeled protein yields a set of N-15-labeled peptides for use as internal standards to identify and quantify Shiga or Shiga-like toxins. We determined that this approach can be used to detect, quantify and distinguish among the known Shiga toxins (Stx) and Shiga-like toxins (Stx1 and Stx2) in the low attomole range (per injection) in complex media, including human serum. Furthermore, Stx1a could be detected and distinguished from the newly identified Stx1e in complex media. As new Shiga-like toxins are identified, this approach can be readily modified to detect them. Since intact toxins are digested with trypsin prior to analysis, the handling of intact Shiga toxins is minimized. The analysis can be accomplished within 5 h. C1 [Silva, Christopher J.; Erickson-Beltran, Melissa L.; Skinner, Craig B.; Patfield, Stephanie A.; He, Xiaohua] USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Silva, CJ (reprint author), USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov; melissa.erickson@ars.usda.gov; craig.skinner@ars.usda.gov; stephanie.patfield@ars.usda.gov; xiaohua.he@ars.usda.gov OI Silva, Christopher/0000-0003-4521-6377 NR 44 TC 0 Z9 0 U1 1 U2 5 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD DEC PY 2015 VL 7 IS 12 BP 5236 EP 5253 DI 10.3390/toxins7124875 PG 18 WC Toxicology SC Toxicology GA CZ3OI UT WOS:000367013500026 PM 26633510 ER PT J AU Manoli, G Bonetti, S Scudiero, E Morari, F Putti, M Teatini, P AF Manoli, G. Bonetti, S. Scudiero, E. Morari, F. Putti, M. Teatini, P. TI Modeling Soil-Plant Dynamics: Assessing Simulation Accuracy by Comparison with Spatially Distributed Crop Yield Measurements SO VADOSE ZONE JOURNAL LA English DT Article ID ELECTRICAL-CONDUCTIVITY; BOUNDARY-CONDITION; ROOT SYSTEMS; WATER; MOISTURE; GROWTH; PHOTOSYNTHESIS; ATMOSPHERE; FARMLAND; CLIMATE AB Coupling hydrological models with plant physiology is crucial to capture the feedback mechanisms occurring within the soil-plant-atmosphere continuum. However, the ability of such models to describe the spatial variability of plant responses to different environmental factors remains to be proven, especially at large scales (field or watershed). We used an innovative three-dimensional soil-plant model to quantify temporal and spatial variability of crop productivity at the field scale, and we assessed simulation accuracy by comparison with spatially distributed crop yield measurements. A 25-ha field located in the Venice coastland, Italy, cultivated with a maize (Zea mays L.) crop and characterized by a highly heterogeneous soil subject to salt contamination, has been extensively studied by soil sampling, geophysical surveys, and hydrological monitoring. Based on these observations, field-scale simulations of soil moisture dynamics coupled with plant transpiration, photosynthesis, and growth were run and compared with crop yield maps of different growing seasons. The model captured the observed crop productivity (grain yield varying between 2 and 15 Mg ha(-1)), but the accuracy of the predicted spatial patterns was limited by the available information on soil heterogeneities. Further model uncertainties are related to the characterization of the rooting systems and their responses to environmental factors (soil characteristics, precipitation) that were shown to be crucial to describe the effect of drought conditions on growth processes. These results demonstrate that large-scale mechanistic simulations of soil-plant systems require a trade-off between site characterization, model processes, and computational efficiency, offering an open challenge for future ecohydrological research. C1 [Manoli, G.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Bonetti, S.] Duke Univ, Pratt Sch Engn, Durham, NC 27708 USA. [Scudiero, E.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Morari, F.] Univ Padua, Dep Agron Food Nat Resources Anim & Environm, I-35121 Padua, Italy. [Putti, M.] Univ Padua, Dep Math, I-35121 Padua, Italy. [Teatini, P.] Univ Padua, Dep Civil Architectural & Environm Engn, I-35131 Padua, Italy. RP Manoli, G (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM gabriele.manoli@duke.edu RI Putti, Mario/I-5996-2014; Scudiero, Elia/K-3580-2013; Putti, Mario/A-5148-2012; OI Putti, Mario/0000-0002-0382-0202; Putti, Mario/0000-0002-0382-0202; Manoli, Gabriele/0000-0002-9245-2877; Scudiero, Elia/0000-0003-4944-721X FU National Science Foundation [NSF-EAR-1344703]; University of Padova, Italy [WP4] FX We acknowledge support from the University of Padova, Italy, within the Research Programme "GEO-RISKS: Geological, morphological and hydrological processes: monitoring, modelling and impact in the north-eastern Italy," WP4; G. Manoli acknowledges support from the National Science Foundation (NSF-EAR-1344703). The data used in this study are available on request to the corresponding author. NR 61 TC 1 Z9 1 U1 3 U2 11 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD DEC PY 2015 VL 14 IS 12 DI 10.2136/vzj2015.05.0069 PG 13 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CZ6QT UT WOS:000367226500002 ER PT J AU Ledig, FT Smouse, PE Hom, JL AF Ledig, F. Thomas Smouse, Peter E. Hom, John L. TI Postglacial migration and adaptation for dispersal in pitch pine (Pinaceae) SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE clutch size; fecundity; glacial refugia; Pinaceae; Pinus rigida; precocity; seed mass; serotiny ID LAST GLACIAL MAXIMUM; EASTERN NORTH-AMERICA; CLIMATE-CHANGE; GENETIC-VARIATION; GEOGRAPHIC PATTERN; PLANT MIGRATION; SEED WEIGHT; GULF-STREAM; CLUTCH SIZE; NEW-ENGLAND AB PREMISE OF THE STUDY: Variation in a species is a blend of adaptive, random, and migratory responses. Pitch pine (Pinus rigida), a highly variable eastern conifer, has occupied multiple glacial refugia, whose harsh conditions favored adaptations enhancing subsequent dispersal and recolonization of newly deglaciated sites. We assessed phenotypic diversity in long-term growth trials to elucidate both the adaptations and likely refugia. METHODS: Pitch pine progeny from 31 areas were grown in common gardens in six locations, from eastern Massachusetts to Korea. KEY RESULTS: Survival increased with source latitude, but seedlings from southern latitudes were tallest in the first (postplanting) year, but that advantage dissipated in later years. Progeny from northern latitudes were precocious, highly fecund, had smaller seeds, and more seeds per cone. Seed mass decreased with latitude in both parents and progeny. Serotinous cones were notably common in the New Jersey Pine Plains and Acadia National Park. Various disease agents and frost burn exhibited latitudinal trends that were nonlinear, with a break in the regression slope at about 40 degrees N latitude. Cluster analysis identified both northern and southern groups, largely split between unglaciated and deglaciated terrain, but with Acadia and the Pine Plains as unique outliers. Within the southern group, provenances were organized into contiguous subgroups, but geographic structure was less evident in the northern group. CONCLUSIONS: The present range of pitch pine was colonized by migrants from at least three different refugia, including at least one on the exposed continental shelf during the Last Glacial Maximum. C1 [Ledig, F. Thomas] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Smouse, Peter E.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [Hom, John L.] US Forest Serv, No Res Stn, USDA, Newtown Sq, PA 19073 USA. RP Smouse, PE (reprint author), Rutgers State Univ, Dept Ecol Evolut & Nat Resources, 14 Coll Farm Rd, New Brunswick, NJ 08901 USA. EM smouse@aesop.rutgers.edu FU American Philosophical Society; Michaux Fund [30, 38]; NSF [GB 42245x, BMS 74-11794, DEB 74-11794]; USDA Forest Service FX Any study of this scope and duration represents the support and cooperation of a great many people. This study was supported with initial funding from the American Philosophical Society, Michaux Fund grants No. 30 and 38 and continued with NSF grants GB 42245x, BMS 74-11794, and DEB 74-11794 to F.T.L. A Franklin Research Grant from the American Philosophical Society enabled F.T.L. to travel to the test sites in 2007 to begin final measurements. Grants to J.L.H. from the USDA Forest Service and to P.E.S. from the New Jersey Agricultural Experiment Station USDA/NJAES-17111 supported measurements in 2008. We are grateful to the late Drs. John H. Fryer, Silas Little, John E. Kuser, Sin-Kyu Hyun, Allan P. Drew, Francois Mergen, and Ralph A. Read for their help on many aspects of this study. All would have been co-authors had they lived. We appreciate the assistance of the Yale University students, graduate students, and postdoctorates who helped plant, maintain, and measure the test sites, including Daniel I. H. Linzer, Diddahally R. Govindaraju, Donald R. Korbobo, Richard C. Beck, Raymond P. Guries, James W. Adams, Clements C. Lambeth, Michael L. Shea, Sharon T. Friedman, and Thomas M. Marino, and to the Rutgers University students who participated in measurements, including Carolyn Haines and Donald R. Knezick. We are especially grateful to Edward C. "Ted" Childs and Starling W. Childs II and their Great Mountain Forest for providing and maintaining one of the most important test sites and to Darrell F. Russ and the Great Mountain Forest crew for measurements over many years. The late Santiago "Sonny" Porcella III of the New Jersey Department of Environmental Protection and the late James J. Toffling of the Massachusetts Department of Natural Resources were indispensable in obtaining and preparing test sites in New Jersey and Massachusetts. The State of Connecticut Department of Environmental Protection grew the seedlings for the tests, and we especially thank nurseryman C. G. "Pete" Merrill. We are grateful to Christian M. Bethmann and other staff of New Jersey Parks and Forestry for their time, advice, and assistance in working with the Pinelands Commission. Dr. Jae-Woo Hwang and other scientists and technicians at the Institute of Forest Genetics, Korea, established and measured the Suwon plantings and subsequently analyzed the results. Finally, thanks to Nicholas S. Skowronski, Matthew M. Patterson, Jason A. Cole, Michael R. Gallagher, and Andrea T. Kornbluh of the USDA Forest Service Northern Research Station; Roger A. Stutts of the Pacific Southwest Research Station; and Linda Lux for their help in the field. Thanks to James A. Baldwin for helpful discussions and for the cluster analyses. Jean Beaulieu, Stephen T. Jackson, Gerald E. Rehfeldt, and two anonymous reviewers made many helpful comments on the manuscript, for which we are very grateful. NR 112 TC 0 Z9 0 U1 9 U2 28 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 EI 1537-2197 J9 AM J BOT JI Am. J. Bot. PD DEC PY 2015 VL 102 IS 12 BP 2074 EP 2091 DI 10.3732/ajb.1500009 PG 18 WC Plant Sciences SC Plant Sciences GA CY8WG UT WOS:000366688800012 PM 26656127 ER PT J AU Scholl, MA Shanley, JB Murphy, SF Willenbring, JK Occhi, M Gonzalez, G AF Scholl, Martha A. Shanley, James B. Murphy, Sheila F. Willenbring, Jane K. Occhi, Marcie Gonzalez, Grizelle TI Stable-isotope and solute-chemistry approaches to flow characterization in a forested tropical watershed, Luquillo Mountains, Puerto Rico SO APPLIED GEOCHEMISTRY LA English DT Article ID RAIN-FOREST; HYDROGRAPH SEPARATION; DEUTERIUM EXCESS; LONG-TERM; RUNOFF; PRECIPITATION; CATCHMENTS; GENERATION; PATTERNS; NITROGEN AB The prospect of changing climate has led to uncertainty about the resilience of forested mountain watersheds in the tropics. In watersheds where frequent, high rainfall provides ample runoff, we often lack understanding of how the system will respond under conditions of decreased rainfall or drought. Factors that govern water supply, such as recharge rates and groundwater storage capacity, may be poorly quantified. This paper describes 8-year data sets of water stable isotope composition (delta H-2 and delta O-18) of precipitation (4 sites) and a stream (1 site), and four contemporaneous stream sample sets of solute chemistry and isotopes, used to investigate watershed response to precipitation inputs in the 1780-ha Rio Mameyes basin in the Luquillo Mountains of northeastern Puerto Rico. Extreme delta H-2 and delta O-18 values from low-pressure storm systems and the deuterium excess (d-excess) were useful tracers of watershed response in this tropical system. A hydrograph separation experiment performed in June 2011 yielded different but complementary information from stable isotope and solute chemistry data. The hydrograph separation results indicated that 36% of the storm rain that reached the soil surface left the watershed in a very short time as runoff. Weathering-derived solutes indicated near-stream groundwater was displaced into the stream at the beginning of the event, followed by significant dilution. The more biologically active solutes exhibited a net flushing behavior. The d-excess analysis suggested that streamflow typically has a recent rainfall component (similar to 25%) with transit time less than the sampling resolution of 7 days, and a more well-mixed groundwater component (similar to 75%). The contemporaneous stream sample sets showed an overall increase in dissolved solute concentrations with decreasing elevation that may be related to groundwater inputs, different geology, and slope position. A considerable amount of water from rain events runs off as quickflow and bypasses subsurface watershed flow-paths, and better understanding of shallow hillslope and deeper groundwater processes in the watershed will require sub-weekly data and detailed transit time modeling. A combined isotopic and solute chemistry approach can guide further studies to a more comprehensive model of the hydrology, and inform decisions for managing water supply with future changes in climate and land use. Published by Elsevier Ltd. C1 [Scholl, Martha A.] US Geol Survey, Natl Res Program, Reston, VA 22092 USA. [Shanley, James B.] US Geol Survey, New Hampshire Vermont Water Sci Ctr, Montpelier, VT USA. [Murphy, Sheila F.] US Geol Survey, Natl Res Program, Boulder, CO USA. [Willenbring, Jane K.; Occhi, Marcie] Univ Penn, Dept Earth & Environm Sci, Philadelphia, PA 19104 USA. [Occhi, Marcie] Virginia Dept Mines Minerals & Energy, Charlottesville, VA USA. [Gonzalez, Grizelle] US Forest Serv, USDA, Int Inst Trop Forestry, Rio Piedras, PR USA. RP Scholl, MA (reprint author), US Geol Survey, Natl Res Program, 959 Natl Ctr, Reston, VA 22092 USA. EM mascholl@usgs.gov RI Willenbring, Jane/B-6431-2011; OI Willenbring, Jane/0000-0003-2722-9537; Scholl, Martha/0000-0001-6994-4614; Gonzalez, Grizelle /0000-0003-3007-5540 FU USGS Climate and Land Use Change Program; NSF Critical Zone Observatories Program [NSF EAR-0722476] FX This work was supported by the USGS Climate and Land Use Change Program, and the NSF Critical Zone Observatories Program Grant NSF EAR-0722476. Carlos Estrada (USFS) and Manuel Rosario and Angel Torres (USGS) helped with field work. We thank Haiping Qi, Jennifer Lorenz and Lauren Tarbox of the USGS Reston Stable Isotope Laboratory for analysis of isotope samples. Michael Doughten, Peggy Widman and Brett Uhle of the USGS analyzed solute chemistry for the contemporaneous sample sets. We thank Bill McDowell and Jody Potter, UNH, for the chemical analyses of the hydrograph separation samples. Comments from M. Alisa Mast of the USGS, and two anonymous reviewers improved this manuscript substantially. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 61 TC 1 Z9 2 U1 5 U2 28 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0883-2927 J9 APPL GEOCHEM JI Appl. Geochem. PD DEC PY 2015 VL 63 BP 484 EP 497 DI 10.1016/j.apgeochem.2015.03.008 PG 14 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA CY2DU UT WOS:000366219800041 ER PT J AU Gese, EM Terletzky, PA AF Gese, Eric M. Terletzky, Patricia A. TI Using the "placeholder" concept to reduce genetic introgression of an endangered carnivore SO BIOLOGICAL CONSERVATION LA English DT Article DE Coyote; Hybrid; Introgression; Placeholder; Red wolf; Sterilization ID WOLF CANIS-RUFUS; NORTH-CAROLINA; HOME-RANGE; SURGICAL STERILIZATION; TERRITORY FIDELITY; SURVIVAL RATES; WILD COYOTES; HABITAT USE; SPACE USE; HYBRIDIZATION AB One of the most endangered species is the red wolf, Canis rufus. Reintroduction of the red wolf began in 1987, but in 1993 hybridization between coyotes (Canis latrans) and wolves was documented. To,reduce genetic introgression, coyotes and coyote-wolf hybrids were captured, sterilized, and released as "placeholders". Placeholders held territories until either displaced or killed by a wolf, or management personnel removed them before releasing a wolf. We evaluated the placeholder concept by examining the number of animals sterilized and released, likelihood of displacement by a wolf, factors influencing displacements, territory fidelity of placeholders, and survival rates and causes of mortality of placeholders and wolves. Of the 182 placeholders, 125 were coyotes and 57 were hybrids. From 1999 to 2013, 51 placeholders were displaced or killed by wolves, and 16 were removed by management personnel. Thus, 37% of the placeholders were displaced leading to occupancy by a wolf. Most displacements occurred in winter (43%) and were always by the same sex. Males were more likely to be displaced than females. Home range characteristics influencing the probability of displacement included home-range size (i.e., more placeholders displaced from larger home ranges) and road density (i.e., more placeholders displaced from home ranges with lower road density). Annual survival of placeholders was higher than wolves in 12 of 14 years, with cause-specific mortality similar among wolves and placeholders. Placeholders provided territories for wolves to colonize, yet reduced the production of hybrid litters, thereby limiting genetic introgression to <4% coyote ancestry in the wolf population. Published by Elsevier Ltd. C1 [Gese, Eric M.] Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. [Terletzky, Patricia A.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. RP Gese, EM (reprint author), Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. EM eric.gese@usu.edu FU U.S. Fish and Wildlife Service FX Funding for all field aspects and data collection was provided by the U.S. Fish and Wildlife Service. Additional support for data analysis and manuscript preparation provided by Point Defiance Zoo and Aquarium, Tacoma, Washington, and the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field Station, Logan, Utah. We gratefully acknowledge U.S. Fish and Wildlife Service personnel associated with the red wolf recovery effort for their diligence in documenting their efforts, locating the animals, and providing access to their data, including A. Beyer, B. Fazio, R. Harrison, D. Hendry, B. Kelly, C. Lucash, F. Mauney, S. McLellan, M. Morse, D. Rabon, L. Schutte, and K. Whidbee. NR 59 TC 5 Z9 5 U1 8 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD DEC PY 2015 VL 192 BP 11 EP 19 DI 10.1016/j.biocon.2015.09.003 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CY6TB UT WOS:000366540600002 ER PT J AU Keith, D Akcakaya, HR Butchart, SHM Collen, B Dulvy, NK Holmes, EE Hutchings, JA Keinath, D Schwartz, MK Shelton, AO Waples, RS AF Keith, David Akcakaya, H. Resit Butchart, Stuart H. M. Collen, Ben Dulvy, Nicholas K. Holmes, Elizabeth E. Hutchings, Jeffrey A. Keinath, Doug Schwartz, Michael K. Shelton, Andrew O. Waples, Robin S. TI Temporal correlations in population trends: Conservation implications from time-series analysis of diverse animal taxa SO BIOLOGICAL CONSERVATION LA English DT Article DE Population growth rate; Population trend; Endangered species; Time series; Vertebrates ID CLIMATE-CHANGE; MARINE FISHES; EXTINCTION; ABUNDANCE; RECOVERY; OSCILLATION; ECOSYSTEM; DENSITY; BIOLOGY AB Population trends play a large role in species risk assessments and conservation planning, and species are often considered threatened if their recent rate of decline meets certain thresholds, regardless how large the population is. But how reliable an indicator of extinction risk is a single estimate of population trend? Given the integral role this decline-based approach has played in setting conservation priorities, it is surprising that it has undergone little empirical scrutiny. We compile an extensive global dataset of time series of abundance data for over 1300 vertebrate populations to provide the first major test of the predictability of population growth rates in nature. We divided each time series into assessment and response periods and examined the correlation between growth rates in the two time periods. In birds, population declines tended to be followed by further declines, but mammals, salmon, and other bony fishes showed the opposite pattern: past declines were associated with subsequent population increases, and vice versa. Furthermore, in these taxa subsequent growth rates were higher when initial declines were more severe. These patterns agreed with data simulated under a null model for a dynamically stable population experiencing density dependence. However, this type of result could also occur if conservation actions positively affected the population following initial declines a scenario that our data were too limited to rigorously evaluate. This ambiguity emphasizes the importance of understanding the underlying causes of population trajectories in drawing inferences about rates of decline in abundance. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Keith, David; Hutchings, Jeffrey A.] Dalhousie Univ, Dept Biol, Halifax, NS B3H 4R2, Canada. [Akcakaya, H. Resit] SUNY Stony Brook, Dept Ecol & Evolut, Stony Brook, NY 11794 USA. [Butchart, Stuart H. M.] BirdLife Int, Wellbrook Court, Cambridge CB30NA, England. [Collen, Ben] UCL, Ctr Biodivers & Environm Res, Dept Genet Evolut & Environm, London WC1E 6BT, England. [Dulvy, Nicholas K.] Simon Fraser Univ, Earth Ocean Res Grp, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada. [Holmes, Elizabeth E.] NOAA Fisheries, Conservat Biol Div, NW Fisheries Sci Ctr, Seattle, WA USA. [Hutchings, Jeffrey A.] Univ Oslo, Dept Biosci, Ctr Ecol & Evolutionary Synth, NO-0316 Oslo, Norway. [Keinath, Doug] Univ Wyoming, Wyoming Nat Div Database, Laramie, WY 82071 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Waples, Robin S.] NOAA Fisheries, NW Fisheries Sci Ctr, Seattle, WA 98112 USA. RP Waples, RS (reprint author), NOAA Fisheries, NW Fisheries Sci Ctr, 2725 Montlake Blvd E, Seattle, WA 98112 USA. EM robin.waples@noaa.gov RI Collen, Ben/F-2543-2016; Waples, Robin/K-1126-2016; OI Collen, Ben/0000-0003-2564-4243; Dulvy, Nicholas/0000-0002-4295-9725 FU National Center for Ecological Analysis and Synthesis (NCEAS), a Center - NSF [EF-0553768]; University of California, Santa Barbara; State of California FX This is a contribution from the 'Red Flags and Species Endangerment' working group, sponsored by the National Center for Ecological Analysis and Synthesis (NCEAS), a Center funded by NSF (Grant #EF-0553768), the University of California, Santa Barbara, and the State of California. We thank the other participants of the Working Group (Priyanga Amarasekare, Jean Cochrane, and Marta Nammack), as well as Brendan Connors, Russ Lande, and Georgina Mace, for their useful discussions and information. NR 53 TC 4 Z9 4 U1 6 U2 35 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD DEC PY 2015 VL 192 BP 247 EP 257 DI 10.1016/j.biocon.2015.09.021 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CY6TB UT WOS:000366540600028 ER PT J AU Oakes, LE Hennon, PE Ardoin, NM D'Amore, DV Ferguson, AJ Steel, EA Wittwer, DT Lambin, EF AF Oakes, Lauren E. Hennon, Paul E. Ardoin, Nicole M. D'Amore, David V. Ferguson, Akida J. Steel, E. Ashley Wittwer, Dustin T. Lambin, Eric F. TI Conservation in a social-ecological system experiencing climate-induced tree mortality SO BIOLOGICAL CONSERVATION LA English DT Article DE Climate change adaptation; Forest dieback; Perspectives on intervention; Conservation triage; Protected areas; Callitropsis nootkatensis ID YELLOW-CEDAR DECLINE; BIODIVERSITY CONSERVATION; SOUTHEASTERN ALASKA; ADAPTATION; RESILIENCE; WILDERNESS; MANAGEMENT; TOLERANCE; FRAMEWORK; RESERVES AB We present a social-ecological framework to provide insight into climate adaptation strategies and diverse perspectives on interventions in protected areas for species experiencing climate-induced impacts. To develop this framework, we examined the current ecological condition of a culturally and commercially valuable species, considered the predicted future effects of climate change on that species in a protected area, and assessed the perspectives held by forest users and managers on future adaptive practices. We mapped the distribution of yellow-cedar (Callitropsis nootkatensis) and examined its health status in Glacier Bay National Park and Preserve by comparing forest structure, tree stress-indicators, and associated thermal regimes between forests inside the park and forests at the current latitudinal limit of the species dieback. Yellow-cedar trees inside the park were healthy and relatively unstressed compared to trees outside the park that exhibited reduced crown fullness and increased foliar damage. Considering risk factors for mortality under future climate scenarios, our vulnerability model indicated future expected dieback occurring within park boundaries. Interviews with forest users and managers revealed strong support for increasing monitoring to inform interventions outside protected areas, improving management collaboration across land designations, and using a portfolio of interventions on actively managed lands. Study participants who perceived humans as separate from nature were more opposed to interventions in protected areas. Linking social and ecological analyses, our study provides an interdisciplinary approach to identify system-specific metrics (e.g., stress indicators) that can better connect monitoring with management, and adaptation strategies for species impacted by climate change. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Oakes, Lauren E.] Stanford Univ, Emmett Interdisciplinary Program Environm & Resou, Stanford, CA 94305 USA. [Hennon, Paul E.; D'Amore, David V.] US Forest Serv, Forestry Sci Lab, Pacific NW Res Stn, USDA, Juneau, AK 99801 USA. [Ardoin, Nicole M.] Stanford Univ, Stanford Woods Inst Environm, Stanford, CA 94305 USA. [Ardoin, Nicole M.] Stanford Univ, Sch Educ, Stanford, CA 94305 USA. [Ferguson, Akida J.; Steel, E. Ashley] US Forest Serv, Pacific NW Res Stn, USDA, Seattle, WA 98103 USA. [Wittwer, Dustin T.] US Forest Serv, USDA, Alaska Reg, Juneau, AK 99801 USA. [Lambin, Eric F.] Stanford Univ, Sch Earth Energy & Environm Sci, Stanford, CA 94305 USA. [Lambin, Eric F.] Stanford Univ, Woods Inst Environm, Stanford, CA 94305 USA. RP Oakes, LE (reprint author), Stanford Univ, Emmett Interdisciplinary Program Environm & Resou, 473 Via Ortega Way,Suite 226, Stanford, CA 94305 USA. EM leoakes@stanford.edu; phennon@fs.fed.us; nmardoin@stanford.edu; ddamore@fs.fed.us; akidajferguson@fs.fed.us; asteel@fs.fed.us; dwittwer@fs.fed.us; elambin@stanford.edu FU US Department of Interior [P11AC90970, J8W07110001]; University of Washington [P11AC90970, J8W07110001]; Morrison Institute for Population and Resource Studies at Stanford University; School of Earth, Energy, and Environmental Sciences at Stanford University; Wilderness Society Gloria Barron Fellowship [10017-32155]; National Forest Foundation [WSC BD-001, WSC BE-002, WSC BF-002]; USDA Forest Service Pacific Northwest Research Station [11-JV-11261937-083]; Forest Health Protection; NSF FX Financial support was provided by the George W. Wright Climate Change Fellowship (Task Agreement #P11AC90970, under Cooperative Agreement J8W07110001, US Department of Interior and the University of Washington); the Morrison Institute for Population and Resource Studies and the School of Earth, Energy, and Environmental Sciences at Stanford University; the Wilderness Society Gloria Barron Fellowship (10017-32155); the National Forest Foundation (WSC BD-001, WSC BE-002, WSC BF-002); and USDA Forest Service Pacific Northwest Research Station (Joint Venture Agreement 11-JV-11261937-083) and Forest Health Protection. The lead author was supported by an NSF Graduate Research Fellowship. Research in GLBA was authorized by the National Park Service under permits GLBA-00192 and 00161. Valuable local knowledge of the study area and assistance with remote logistics were provided by L. Sharman, P. Johnson, and G. Streveler, who also reviewed our yellow-cedar distribution mapping. We thank Kevin O'Hara for his feedback on plot data analysis and the application of our organizing framework to other systems; field assistants K. Cahill, P. Fischer, O. Miller, T. Ward, C. Radis, and G. Treinish; coding assistants C. Woolsey and R. Malczynski; and many community members throughout southeast Alaska whose support made this study possible. Adventurers and Scientists for Conservation reclaimed sensors in WCYW with support from S. Harris and the Sitka Conservation Society. We thank Captain Z. Stenson and his volunteers who reclaimed temperature sensors in GLBA. F. Biles, USDA Forest Service geographer at the Pacific Northwest Research Station in Juneau, Alaska, developed the topographic wetness index. We are grateful for the interviewees who generously shared their perspectives, time, and hospitality. K. Cahill contributed tree drawing to Fig. I. Comments from A. Cravens and F. Moore improved this manuscript, and W. Hoover provided copyediting assistance. NR 55 TC 2 Z9 2 U1 5 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD DEC PY 2015 VL 192 BP 276 EP 285 DI 10.1016/j.biocon.2015.09.018 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CY6TB UT WOS:000366540600031 ER PT J AU Yu, N Laurenz, R Siler, L Ng, PKW Souza, E Lewis, JM AF Yu, N. Laurenz, R. Siler, L. Ng, P. K. W. Souza, E. Lewis, J. M. TI Evaluation of alpha-Amylase Activity and Falling Number around Maturity for Soft White and Soft Red Wheat Varieties in Michigan SO CEREAL RESEARCH COMMUNICATIONS LA English DT Article DE falling number; wheat; alpha-amylase; Michigan; pre-harvest sprouting; PHS; red wheat; white wheat; quality; breeding ID PREHARVEST SPROUTING RESISTANCE; GRAIN DORMANCY; WINTER-WHEAT; ASSAY; EMBRYO; DAMAGE; L. AB White wheat is, categorically, more susceptible to pre-harvest sprouting (PHS) than red wheat. Physiological maturity (PM), defined as when the seeds reach their maximum dry weight, is a critical time before harvesting. The objective of this study was to determine a reference level of alpha-amylase activity and the corresponding Falling Number (FN) value near the time of PM of selected red and white cultivars in the absence of PHS inducing conditions. Twenty-four soft winter wheat genotypes (12 red and 12 white) adapted to Michigan with varying historic levels of susceptibility to PHS were planted in an alpha-lattice design in two locations from 2008 to 2010. Spikes were collected three days before PM, at PM, and three days post PM. Samples were freeze-dried, threshed, milled and evaluated for alpha-amylase activity and FN value using high throughput method. Within genotype, clear trends were observed in the reduction of alpha-amylase activity and the increase of FN value during the physiological maturation. A nonlinear relationship between alpha-amylase activity and FN value was fit with an r(2) of 0.801. Significant differences were observed for genotype for both alpha-amylase activity and FN value for all collection time points. No significant differences were found between red and white wheat, categorically, at any of the three time-points in the absence of PHS. The evaluation results provide a critical reference prior to induction of PHS. The alpha-amylase activity and FN tests show different advantages in analyzing PHS samples as the relationship between alpha-amylase activity and FN value is not linear over wide-ranging results. C1 [Yu, N.; Laurenz, R.; Siler, L.; Lewis, J. M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Ng, P. K. W.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Souza, E.] USDA ARS, Soft Wheat Qual Lab, Wooster, OH 44691 USA. RP Lewis, JM (reprint author), Bayer CropSci LP, Lincoln, NE 68521 USA. EM janet.lewis@bayer.com FU Eastern Soft White Wheat Endowment Committee; Michigan State Miller's Association; Michigan Crop Improvement Association FX This work was supported by Eastern Soft White Wheat Endowment Committee, Michigan State Miller's Association and Michigan Crop Improvement Association. NR 25 TC 0 Z9 0 U1 9 U2 24 PU AKADEMIAI KIADO RT PI BUDAPEST PA PRIELLE K U 19, PO BOX 245,, H-1117 BUDAPEST, HUNGARY SN 0133-3720 EI 1788-9170 J9 CEREAL RES COMMUN JI Cereal Res. Commun. PD DEC PY 2015 VL 43 IS 4 BP 672 EP 681 DI 10.1556/0806.43.2015.026 PG 10 WC Agronomy SC Agriculture GA CY4UO UT WOS:000366404300013 ER PT J AU Power, TG O'Connor, TM Fisher, JO Hughes, SO AF Power, Thomas G. O'Connor, Teresia M. Fisher, Jennifer Orlet Hughes, Sheryl O. TI Obesity Risk in Children: The Role of Acculturation in the Feeding Practices and Styles of Low- Income Hispanic Families SO CHILDHOOD OBESITY LA English DT Article ID WEIGHT STATUS; GREATER ACCULTURATION; CHILDHOOD OBESITY; ADOLESCENT HEALTH; SCHOOL-CHILDREN; MEXICAN-ORIGIN; US; OVERWEIGHT; BEHAVIORS; AMERICAN AB Background: Parent feeding has been associated with child overweight/obesity in low-income families. Because acculturation to the United States has been associated with increased adult obesity, our study aim was to determine whether acculturation was associated with feeding in these populations. Methods: Low-income Hispanic mothers of preschoolers were recruited to participate in a longitudinal study examining child eating behaviors. At baseline, mothers completed questionnaires on feeding styles, feeding practices, and acculturation. Regression analyses compared feeding styles and food parenting practices of first-generation, immigrant mothers born outside the United States (n=138) and mothers born in the United States (n=31). The correlates of acculturation with these same constructs were also examined. Results: Immigrant mothers reported using highly directive food parenting practices more often than mothers born in the United States, including pressuring their child to consume more food, using food as a reward, and controlling child food intake by limiting less-healthy foods. First-generation mothers were more likely to show authoritarian, and less likely to show indulgent, feeding styles. Greater maternal acculturation was associated with less restriction of food for weight reasons. Conclusions: Although first-generation, immigrant mothers reported using highly controlling food parenting practices with their children, those born in the United States were more indulgent with their children in the feeding context. Mechanisms that promote greater indulgence in more-acculturated mothers need to be identified. C1 [Power, Thomas G.] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. [O'Connor, Teresia M.; Hughes, Sheryl O.] Baylor Univ, Dept Pediat, USDA ARS, Baylor Coll Med,Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Fisher, Jennifer Orlet] Temple Univ, Dept Publ Hlth, Ctr Obes Res & Educ, Philadelphia, PA 19122 USA. RP Hughes, SO (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, Pediat, 1100 Bates St, Houston, TX 77030 USA. EM shughes@bcm.edu FU National Institute of Child Health and Human Development [R01 HD062567]; USDA (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) [58-6250-0-008] FX This research was supported by funds from the National Institute of Child Health and Human Development (grant no.: R01 HD062567). This work is also a publication of the USDA (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) funded, in part, by the USDA/ARS (Cooperative Agreement 58-6250-0-008). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. The authors acknowledge the following people who were instrumental in collecting and the data for this study: Nilda Micheli and Monica Lopez. NR 42 TC 2 Z9 2 U1 6 U2 18 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD DEC 1 PY 2015 VL 11 IS 6 BP 715 EP 721 DI 10.1089/chi.2015.0036 PG 7 WC Pediatrics SC Pediatrics GA CY5PP UT WOS:000366459900008 PM 26584157 ER PT J AU Richardson, K Schnitzler, GR Lai, CQ Ordovas, JM AF Richardson, Kris Schnitzler, Gavin R. Lai, Chao-Qiang Ordovas, Jose M. TI Functional Genomics Analysis of Big Data Identifies Novel Peroxisome Proliferator-Activated Receptor. Target Single Nucleotide Polymorphisms Showing Association With Cardiometabolic Outcomes SO CIRCULATION-CARDIOVASCULAR GENETICS LA English DT Article DE adipocytes; cardiovascular disease; genetics; genetic polymorphism; genomics; type 2 diabetes mellitus ID CORONARY-ARTERY-DISEASE; PPAR-GAMMA; NATURAL-SELECTION; CANDIDATE GENES; BINDING-SITES; VARIANTS; DNA; METABOLISM; ELEMENTS; BIOLOGY AB Background Cardiovascular disease and type 2 diabetes mellitus represent overlapping diseases where a large portion of the variation attributable to genetics remains unexplained. An important player in their pathogenesis is peroxisome proliferator-activated receptor (PPAR) that is involved in lipid and glucose metabolism and maintenance of metabolic homeostasis. We used a functional genomics methodology to interrogate human chromatin immunoprecipitation-sequencing, genome-wide association studies, and expression quantitative trait locus data to inform selection of candidate functional single nucleotide polymorphisms (SNPs) falling in PPAR motifs. Methods and Results We derived 27328 chromatin immunoprecipitation-sequencing peaks for PPAR in human adipocytes through meta-analysis of 3 data sets. The PPAR consensus motif showed greatest enrichment and mapped to 8637 peaks. We identified 146 SNPs in these motifs. This number was significantly less than would be expected by chance, and Inference of Natural Selection from Interspersed Genomically coHerent elemenTs analysis indicated that these motifs are under weak negative selection. A screen of these SNPs against genome-wide association studies for cardiometabolic traits revealed significant enrichment with 16 SNPs. A screen against the MuTHER expression quantitative trait locus data revealed 8 of these were significantly associated with altered gene expression in human adipose, more than would be expected by chance. Several SNPs fall close, or are linked by expression quantitative trait locus to lipid-metabolism loci including CYP26A1. Conclusions We demonstrated the use of functional genomics to identify SNPs of potential function. Specifically, that SNPs within PPAR motifs that bind PPAR in adipocytes are significantly associated with cardiometabolic disease and with the regulation of transcription in adipose. This method may be used to uncover functional SNPs that do not reach significance thresholds in the agnostic approach of genome-wide association studies. C1 [Richardson, Kris; Lai, Chao-Qiang; Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer United States Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Schnitzler, Gavin R.] Tufts Med Ctr, Mol Cardiol Res Inst, Boston, MA USA. [Ordovas, Jose M.] Ctr Nacl Invest Cardiovasc, Dept Clin Invest, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac, Dept Nutr Genom, Madrid, Spain. RP Richardson, K (reprint author), Tufts Univ, Nutr & Genom Lab, JM USDA HNRCA, 711 Washington St, Boston, MA 02111 USA. EM kris.richardson@tufts.edu FU US Department of Agriculture Research [53-K06-5-10, 58-1950-9-001] FX This work has been funded by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research. NR 52 TC 0 Z9 0 U1 3 U2 15 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1942-325X EI 1942-3268 J9 CIRC-CARDIOVASC GENE JI Circ.-Cardiovasc. Genet. PD DEC PY 2015 VL 8 IS 6 BP 842 EP 851 DI 10.1161/CIRCGENETICS.115.001174 PG 10 WC Cardiac & Cardiovascular Systems; Genetics & Heredity SC Cardiovascular System & Cardiology; Genetics & Heredity GA CY7QX UT WOS:000366604900011 PM 26518621 ER PT J AU Dai, Z Johnson, KD Birdsey, RA Hernandez-Stefanoni, JL Dupuy, JM AF Dai, Z. Johnson, K. D. Birdsey, R. A. Hernandez-Stefanoni, J. L. Dupuy, J. M. TI Assessing the effect of climate change on carbon sequestration in a Mexican dry forest in the Yucatan Peninsula SO ECOLOGICAL COMPLEXITY LA English DT Article DE Secondary forest; Karstic landscape; Biomass; Soil carbon pool; Forest-DNDC; Biome-BGC ID CLASSIC MAYA CIVILIZATION; PROCESS-ORIENTED MODEL; SENSITIVITY-ANALYSIS; EUCALYPTUS-GLOBULUS; BIOME-BGC; WETLAND ECOSYSTEMS; NO EMISSIONS; DYNAMICS; BALANCE; WATER AB Assessing the effect of climate change on carbon sequestration in tropical forest ecosystems is important to inform monitoring, reporting, and verification (MRV) for reducing deforestation and forest degradation (REDD), and to effectively assess forest management options under climate change. Two process-based models, Forest-DNDC and Biome-BGC, with different spatial modeling scales were evaluated to estimate the potential effect of climate change on carbon sequestration in a tropical dry semi-deciduous forest in the Yucatan Peninsula of Mexico. The results from the simulations using the two models show that carbon sequestration in this dry forest is highly sensitive to warming. Carbon uptake in this forest may increase or decrease slightly with a corresponding increase or decrease in precipitation; however, with an increase in temperature, carbon uptake may decrease significantly, showing that warming may be the main climate factor that impacts carbon storage in this tropical dry forest. Model performance evaluation indicates that both models may be used to estimate C stocks, but DNDC may be better than BGC for assessing the effect of climate change on C dynamics. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dai, Z.; Johnson, K. D.; Birdsey, R. A.] USDA Forest Serv, Newtown Sq, PA 19073 USA. [Dai, Z.] Commiss Environm Cooperat, Montreal, PQ H2Y 1N9, Canada. [Hernandez-Stefanoni, J. L.; Dupuy, J. M.] Ctr Invest Cient Yucatan AC, Unidad Recursos Nat, Merida, Yucatan, Mexico. RP Dai, Z (reprint author), USDA Forest Serv, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM zdai@fs.fed.us NR 65 TC 0 Z9 0 U1 6 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1476-945X EI 1476-9840 J9 ECOL COMPLEX JI Ecol. Complex. PD DEC PY 2015 VL 24 BP 46 EP 56 DI 10.1016/j.ecocom.2015.09.004 PG 11 WC Ecology SC Environmental Sciences & Ecology GA CZ0GI UT WOS:000366782700005 ER PT J AU Pham, TT Loft, L Bennett, K Phuong, VT Dung, LN Brunner, J AF Thu Thuy Pham Loft, Lasse Bennett, Karen Vu Tan Phuong Le Ngoc Dung Brunner, Jake TI Monitoring and evaluation of Payment for Forest Environmental Services in Vietnam: From myth to reality SO ECOSYSTEM SERVICES LA English DT Article DE Ecosystem services; Monitoring and evaluation; Payments for Environmental Services; Vietnam ID ECOSYSTEM SERVICES; COUNTRIES; LANDSCAPES; CHALLENGE; EFFICIENT; LESSONS; ISSUES; CARBON; MEXICO; REDD AB Payments for Ecosystem Services schemes, or PES, offer a useful approach to account for the largely unrecognized value of ecosystem services provided by the forestry sector. However, the actual impacts of PES schemes, both in effectively protecting the environmental quality of an ecosystem, such as the water filtration capacity of a forest, and on improving local livelihoods, often remain unknown. Policy evaluation in general, and for newly established policies in particular, plays a critical role in providing essential feedback about what is actually happening on the ground. Thus, for PES to generate outcomes that are effective, efficient and equitable, policy makers must develop a functional evaluation system. As PES schemes around the world face similar problems in monitoring and evaluation, we draw on the case of Vietnam the first country in Asia to introduce a nationwide PES scheme and analyze the effectiveness of the monitoring and evaluation activities of the Payment for Forest Environmental Services program (PFES). We also offer practical policy recommendations for future PEES implementation. We find that monitoring and evaluation of PEES in Vietnam is still in its infancy. Although there is a strong accounting of revenues generated from ecosystem services buyers, there is a discernible lack of ability to assess the quantity and quality of ecosystem services being enhanced by the program; fulfillment of contractual obligations; the appropriateness of financial flows; or socio-economic impacts of the program. We argue that a functioning PES evaluation system must include an accessible grievance mechanism to ensure transparency and accountability in the distribution of PES revenues from central to local levels. (C) 2015 Elsevier B.V. All rights reserved. C1 [Thu Thuy Pham] Ctr Int Forestry Res, Hanoi 10000, Vietnam. [Bennett, Karen] USDA Forest Serv, Reg Off, Portland, OR 97204 USA. [Vu Tan Phuong] Vietnamese Acad Forest Sci Dong Ngac Commune, Hanoi 10000, Vietnam. [Brunner, Jake] Int Union Conservat Nat, Hanoi 10000, Vietnam. [Loft, Lasse] Leibniz Ctr Agr Landscape Res ZALF, Inst Socioecon, D-15374 Muncheberg, Germany. RP Pham, TT (reprint author), Ctr Int Forestry Res, 17A Nguyen Khang St, Hanoi 10000, Vietnam. EM t.pham@cgiar.org; lasse.loft@t-online.de; kabennett@fs.fed.us; phuong.vt@vafs.gov.vn; t.pham@cgiar.org; jake.brunner@iucn.org NR 54 TC 1 Z9 1 U1 11 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-0416 J9 ECOSYST SERV JI Ecosyst. Serv. PD DEC PY 2015 VL 16 BP 220 EP 229 DI 10.1016/j.ecoser.2015.10.016 PG 10 WC Ecology; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA CY7HA UT WOS:000366578000024 ER PT J AU Alloatti, J Chen, PY Zeng, AL Li, SX Rupe, J Florez-Palacios, L Orazaly, M AF Alloatti, Julieta Chen, Pengyin Zeng, Ailan Li, Shuxian Rupe, John Florez-Palacios, Liliana Orazaly, Moldir TI Inheritance of and molecular markers for purple seed stain resistance in soybean SO EUPHYTICA LA English DT Article DE Soybean; Purple seed stain; Simple sequence repeat; Resistance gene ID QUANTITATIVE TRAIT LOCI; CERCOSPORA-KIKUCHII; TEMPERATURE; GERMINATION; HERITABILITY; INFECTION; DURATION; RICE AB Purple seed stain (PSS), caused by Cercospora kikuchii, is a disease that causes seed quality deterioration in soybean. Breeding for genetic resistance is a cost-efficient and practical way to control the disease. The objectives of this research were to investigate the inheritance of resistance to PSS and identify SSR markers tightly linked to the major resistance gene Rpss1. Two populations were developed by crossing a PSS resistant line, PI 80837, to PSS susceptible lines AP 350 andMO/PSD-0259. F-2:5 lines from each cross were grown at Kibler, AR in a randomized complete block design with three replications. Each plot was harvested and seed samples were taken to evaluate the percentages of visual PSS (% PSS) and C. kikuchii infection (% C. kikuchii). Ranges, LSMeans, and confidence intervals of the parents were used to classify resistant and susceptible reactions in the F-2:5 lines for the two variables evaluated. Sixteen SSR markers in a 17.1 cM region on chromosome (Chr.) 18 were used to screen the parents and both F-2:5 populations. Significant differences in % PSS and % C. kikuchii were observed for the parents and both F-2:5 populations. For the two variables evaluated, both populations showed a good fit to a ratio of 15:1 (resistant:susceptible), indicating two dominant gene action. One chromosomal region in the vicinity of Satt115 and Satt340 on Chr. 18 was determined to be associated with the resistance gene Rpss1 in both populations. These results confirm the presence of a major resistance gene, Rpss1, in PI 80837 and also indicate an additional putative gene for PSS resistance. C1 [Alloatti, Julieta; Chen, Pengyin; Zeng, Ailan; Florez-Palacios, Liliana; Orazaly, Moldir] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Li, Shuxian] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Rupe, John] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. RP Chen, PY (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. EM pchen@uark.edu NR 29 TC 0 Z9 0 U1 3 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD DEC PY 2015 VL 206 IS 3 BP 701 EP 709 DI 10.1007/s10681-015-1492-2 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CY8BK UT WOS:000366633200013 ER PT J AU Bondalapati, KD Jenkins, JN McCarty, JC Wu, JX AF Bondalapati, Krishna D. Jenkins, Johnie N. McCarty, Jack C. Wu, Jixiang TI Field experimental design comparisons to detect field effects associated with agronomic traits in upland cotton SO EUPHYTICA LA English DT Article DE Field variation; Linear mixed model approaches; Variance components; Heritability; Upland cotton ID STATISTICAL-ANALYSIS; INCOMPLETE BLOCK; NEIGHBOR MODEL; VARIETY TRIALS; YIELD; DOMINANCE; WHEAT; LINES AB Field variation is one of the important factors that can have a significant impact on genetic data analysis. Ineffective control of field variation may result in an inflated residual variance and/or biased estimation of genetic variations and/or effects. In this study, we addressed this problem by merging genetic models with the information from a rectangular cotton field layout (referred to row and column directions). Data from a genetic mapping study in Upland cotton (Gossypium hirsutum L.) was used to validate the proposed methodology. This study included model evaluation based on simulations and actual data analysis on four agronomic traits (seed yield, lint yield, lint percentage, and boll weight) in cotton. Results based on simulations suggested that when there were no row and column effects, the conventional and the extended genetic models yielded similar results. However, when either field row and/or column effects were significant, the conventional genetic model yielded biased estimates for residual variance component with larger mean square error whereas the extended genetic models yielded more unbiased estimates. Actual data analysis revealed that lint yield and seed yield were significantly influenced by the systematic variation present in the field. With the extended model, the residual variance associated with these traits was reduced approximately 65 % compared to the conventional block model. Accordingly, the averaged heritability estimate increased by about 18 % for these traits. Thus, the results suggested that genetic data analysis can be improved when field variation is considered. C1 [Bondalapati, Krishna D.] S Dakota State Univ, Dept Math & Stat, Brookings, SD 57007 USA. [Jenkins, Johnie N.; McCarty, Jack C.] USDA ARS, Crop Sci Res Lab, Mississippi State, MS 39762 USA. [Wu, Jixiang] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. RP Wu, JX (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM jixiang.wu@sdstate.edu FU USDA-NIFA [1005459]; South Dakota Agricultural Experiment Station FX This research was partially supported by funding USDA-NIFA Hatch project 1005459 and the South Dakota Agricultural Experiment Station. NR 28 TC 0 Z9 0 U1 1 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD DEC PY 2015 VL 206 IS 3 BP 747 EP 757 DI 10.1007/s10681-015-1512-2 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CY8BK UT WOS:000366633200017 ER PT J AU Jones, ZG Goodman, MM Krakowsky, MD AF Jones, Zachary G. Goodman, Major M. Krakowsky, Matthew D. TI Identification of resistance to the Ga1-m gametophyte factor in maize SO EUPHYTICA LA English DT Article DE Gametophyte Factors; Ga1; Maize; Popcorn ID REPRODUCTIVE ISOLATION AB Due to maize's wind-driven pollination, non-target pollen contamination is problematic for producers and breeders. Maize gametophyte factors, specifically gametophyte factor 1 (ga1), have long been used to produce selectively pollinating phenotypes. The use of these factors is a cornerstone of commercial popcorn production, and they are used for a large range of other purposes, including preventing contamination by genetically modified pollen in organic production. However this system is at great risk from another allele at the ga1 locus, Ga1-m, which overcomes the selectively pollinating phenotypes. To further complicate this problem, the risk posed by this allele has been under-assessed. Here we reinterpret the key study on Ga1-s and report genetic resistance to the Ga1-m allele in maize lines that carry dominant gametophyte factors. We identified genetic resistance to the allele segregating in lines derived from four landraces, showed the resistance is heritable, and that it acts in full-strength and attenuated versions. Additionally, we have suggested the validity of evolutionary-based inquiry into our plant genetic resources, and provided some validation of this effort. Our results provide the first report of effective genetic resistance to pollination by the Ga1-m allele, providing an option to continue the use of genetic barriers to non-target pollination. A source of resistance to the Ga1-m allele allows research to be conducted about the allele itself, allowing for research into the possible existence of multiple versions of the allele and their distributions. We anticipate our research will be a starting point for identification of additional sources of resistance to the Ga1-m allele, specifically in popcorn production, where it is most immediately needed to prevent pollen contamination, as well as the eventual localization and mapping of the resistance alleles. We also believe the suggestion of evolutionary-based inquiry into plant genetic resources will provide a highly effective method for identification of specific traits, but will need more extensive validation. C1 [Jones, Zachary G.; Goodman, Major M.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Krakowsky, Matthew D.] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. RP Jones, ZG (reprint author), N Carolina State Univ, Dept Crop Sci, 101 Derieux Pl,1236 Williams Hall,Box 7620, Raleigh, NC 27695 USA. EM zgjones@ncsu.edu FU Clif Bar Family Foundation FX National Institute for Food and Agriculture ( NIFA) (#2012-51300-20024). Clif Bar Family Foundation for their support of the Seed Matters Fellowship in Plant Breeding supporting Zachary. Jose de Jesus Sanchez, University of Guadalajara. Jerry Kermicle, University of Wisconsin. NR 16 TC 2 Z9 2 U1 1 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD DEC PY 2015 VL 206 IS 3 BP 785 EP 791 DI 10.1007/s10681-015-1518-9 PG 7 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CY8BK UT WOS:000366633200020 ER PT J AU Zeng, LH Campbell, BT Bechere, E Dever, JK Zhang, JF Jones, AS Raper, TB Hague, S Smith, W Myers, GO Bourland, FM AF Zeng, Linghe Campbell, B. Todd Bechere, Efrem Dever, Jane K. Zhang, Jinfa Jones, Andrea S. Raper, Tyson B. Hague, Steve Smith, Wayne Myers, Gerald O. Bourland, Fred M. TI Genotypic and environmental effects on cottonseed oil, nitrogen, and gossypol contents in 18 years of regional high quality tests SO EUPHYTICA LA English DT Article DE Cotton; Cotton seed traits; Gossypol; Nitrogen content; Oil content ID (+)-GOSSYPOL; (-)-GOSSYPOL; INHERITANCE AB Determination of environmental influence on seed traits is critical for genetic improvement of seed quality in upland cotton (Gossypium hirsutum L.). The objective of this study was to analyze the relative contribution of environment and genotype (G) for seed oil, nitrogen (N), and gossypol contents using historical data from the regional high quality (RHQ) tests conducted from 1996 through 2013. The 18-year tests of RHQ were divided into six 3-year cycles with an average of about 20 genotypes and 7-10 testing locations (loc) in each cycle. Variance components of oil, N, and gossypol contents were estimated in each cycle and expressed as percentages of the total variance. Highly significant G x loc effects were identified for all seed quality traits in each cycle. For oil content, variance estimates of G to the total variance ranged from 20 to 57 % in different cycles. For N content, loc was the main source of variance with variance estimates of loc to the total variance ranging from 44 to 73 % in different cycles. In most cycles, loc and G were the main source of variance for free-gossypol content. For most seed quality traits, there was not a clear trend of changes among testing cycles for the variance estimates of G to the total variance. Broad-sense heritability for oil content, N content, and free-gossypol ranged from 0.79 to 0.96, 0.65 to 0.86, and 0.28 to 0.93, respectively. Highly significant G x loc interactions indicate that multiple location trials for testing seed quality traits are necessary. However, heritability estimates for these seed traits indicate stability across environments as well as the potential for genetic improvement. Significant reduction in seed index was observed in half of the testing cycles with a range of 10.4-9.52 within cycles. Correlation between seed index and oil content was positive with r values ranging from 0.23 to 0.77 in different cycles. C1 [Zeng, Linghe; Bechere, Efrem] USDA ARS, Delta Res Ctr, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Campbell, B. Todd] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Dever, Jane K.] Texas A&M Univ, Texas A&M Aglife Ctr, Lubbock, TX 79403 USA. [Zhang, Jinfa] New Mexico State Univ, Las Cruces, NM 88003 USA. [Jones, Andrea S.] Univ Missouri, Coll Agr Food & Nat Resources, Agr Expt Stn, Portageville, MO 63873 USA. [Raper, Tyson B.] Univ Tennessee, West TN Res & Educ Ctr, Dept Plant Sci, Jackson, TN 38301 USA. [Hague, Steve; Smith, Wayne] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Myers, Gerald O.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Bourland, Fred M.] Univ Arkansas, Northeast Res & Extens Ctr, Keiser, AR 72351 USA. RP Zeng, LH (reprint author), USDA ARS, Delta Res Ctr, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM Linghe.zeng@ars.usda.gov FU USDA-ARS [6402-21000-051-00D] FX This research was funded by USDA-ARS, Project No. 6402-21000-051-00D. USDA is an equal opportunity provider and employer. We thank Ellen Keene and Patricia Maugh, Crop Genetics Research Unit at Stoneville, MS for their excellent technical assistance. NR 29 TC 2 Z9 3 U1 4 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD DEC PY 2015 VL 206 IS 3 BP 815 EP 824 DI 10.1007/s10681-015-1523-z PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CY8BK UT WOS:000366633200023 ER PT J AU McHugh, T AF McHugh, Tara TI How Yogurt Is Processed SO FOOD TECHNOLOGY LA English DT Article C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 4 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD DEC PY 2015 VL 69 IS 12 BP 70 EP 72 PG 3 WC Food Science & Technology SC Food Science & Technology GA CY5JN UT WOS:000366444000014 ER PT J AU Picklo, MJ Newman, JW AF Picklo, Matthew J., Sr. Newman, John W. TI Antioxidant supplementation and obesity have independent effects on hepatic oxylipin profiles in insulin-resistant, obesity-prone rats SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE Insulin resistance; alpha-Tocopherol; Obesity; Epoxide hydrolase; Lipoxygenase; Vitamin C; Oxylipins; Cytochrome P450 ID EPOXIDE HYDROLASE DEFICIENCY; VASCULAR ENDOTHELIAL-CELLS; VITAMIN-E; OXIDATIVE STRESS; KINETIC INVESTIGATIONS; GLUCOSE-HOMEOSTASIS; METABOLIC SYNDROME; DIABETIC-PATIENTS; ARACHIDONIC-ACID; OXIDANT STRESS AB Obesity-induced changes in lipid metabolism are mechanistically associated with the development of insulin resistance and prediabetes. Recent studies have focused on the extent to which obesity-induced insulin resistance is mediated through oxylipins, derived from enzymatic and nonenzymatic lipid peroxidation. Vitamin E and vitamin C are widely used antioxidant supplements, but conflicting data exist as to whether supplementation with vitamins E and C reduces insulin resistance. The purpose of this work is (1) to test the hypothesis that supplementation with vitamin E and vitamin C prevents the development of insulin resistance and (2) to determine the extent to which antioxidant supplementation modifies obesity-induced changes in hepatic oxylipins. Using obesity-prone Sprague-Dawley rats fed a high-fat, hypercaloric diet, we found that vitamin E and C supplementation did not block the development of insulin resistance, despite increased plasma levels of these antioxidants and decreased hepatic F-2-isoprostane (F-2-lsoP) concentrations. The obese phenotype was associated with increased hepatic concentrations of cytochrome P450 (CYP450)-dependent linoleic acid and alpha-linolenic acid-derived epoxides. Antioxidant supplementation, but not obesity, decreased levels of the lipoxygenase (LOX)-dependent, arachidonic acid-derived products lipoxin A4 (LXA(4)), 8,15-dihydroxtetraenoate (8,15-DiHETE), and 5,15-DiHETE. Our data demonstrate that antioxidant supplementation and obesity impact hepatic LOX- and CYP450-dependent oxylipin metabolism. Published by Elsevier Inc. C1 [Picklo, Matthew J., Sr.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Picklo, Matthew J., Sr.] Univ N Dakota, Dept Chem, Grand Forks, ND 58201 USA. [Newman, John W.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Picklo, MJ (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. EM matthew.picklo@ars.usda.gov FU USDA-ARS [5450-51000-048-00D, 2032-51530-022-00D] FX The authors thank Maret Traber (Linus Pauling Institute) for her advice regarding interpretation of the plasma alpha-tocopherol data, and Joseph Idso, Kim Michelsen, and Karan Agrawal for their excellent technical assistance. Funding was provided through USDA-ARS Projects 5450-51000-048-00D (MJP) and 2032-51530-022-00D OWN). The U.S. Department of Agriculture, Agricultural Research Service, Plains Area and Pacific West Area, are equal opportunity/affirmative action employers and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 52 TC 1 Z9 1 U1 1 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0891-5849 EI 1873-4596 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PD DEC PY 2015 VL 89 BP 182 EP 191 DI 10.1016/j.freeradbiomed.2015.07.152 PG 10 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA CY4CI UT WOS:000366355800017 PM 26398714 ER PT J AU Gillette, GL Reese, KP Connelly, JW Colt, CJ Knetter, JM AF Gillette, Gifford L. Reese, Kerry P. Connelly, John W. Colt, Chris J. Knetter, Jeffrey M. TI Evaluating the Potential of Aerial Infrared as a Lek Count Method for Prairie Grouse SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE aerial infrared; aerial surveys; Columbian sharp-tailed grouse; infrared camera; lek counts; management program; prairie grouse ID GREATER SAGE-GROUSE; CHICKEN LEKS; POPULATIONS; ABUNDANCE; CANADA AB Wildlife biologists use counts of birds attending traditional breeding grounds (i.e., leks) to develop indices of population trends in several grouse species. Standardized lek counts for sage-grouse Centrocercus spp. provide information about population trends by allowing comparison of counts across their range. In contrast, biologists lack a standard lek-count method for prairie grouse Tympanuchus spp.. The lack of a standard lek-count method limits our ability to make rigorous spatial and temporal comparisons or to estimate population trends. Recent use of cooled infrared cameras in aerial surveys and their increased affordability make this technology attractive for monitoring prairie grouse populations. Our objective was to evaluate the efficacy of aerial infrared (AIR) technology for estimating abundance of prairie grouse by comparing AIR lek counts with ground-based lek counts of Columbian sharp-tailed grouse T. phasianellus columbianus in Idaho. We used both methods simultaneously to count sharp-tailed grouse at 25 leks to compare method consistency. We also used both methods to count sharp-tailed grouse at 88 other leks to quantify and compare the resources required for both methods. The average count obtained with the AIR method (12.3 grouse +/- 1.5 SE) and the ground-based method (12.9 grouse +/- 1.8 SE) was similar, and we were unable to detect a statistical difference between methods when conducting a negative binomial regression (z = 0.165, P = 0.87). Aerial infrared was twice the cost of the ground-based method, but AIR surveyed more leks in less time (88 leks during 4 d) compared with the ground-based method (88 leks during 29 d). Aerial infrared improves population monitoring by counting leks inaccessible by ground. The time efficiency of AIR and the ability to obtain counts consistent with ground-based methods suggests that AIR may be an effective and efficient lek-count method. C1 [Gillette, Gifford L.; Reese, Kerry P.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Connelly, John W.] Univ Idaho, Idaho Cooperat Wildlife Res Unit, Moscow, ID 83844 USA. [Colt, Chris J.] US Forest Serv, Curlew Natl Grasslands, Pocatello, ID 83204 USA. [Knetter, Jeffrey M.] Idaho Dept Fish & Game, Boise, ID 83707 USA. RP Gillette, GL (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, 875 Perimeter Dr,MS 1136, Moscow, ID 83844 USA. EM gill8676@vandals.uidaho.edu FU Idaho Department of Fish and Game (IDFG); U.S. Forest Service (USFS); National Wild Turkey Federation; Conservation Innovation Grant - Idaho Natural Resources Conservation Service FX This research was funded by the Idaho Department of Fish and Game (IDFG), U.S. Forest Service (USFS), the National Wild Turkey Federation, and a Conservation Innovation Grant awarded by the Idaho Natural Resources Conservation Service. NR 40 TC 1 Z9 1 U1 2 U2 6 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD DEC PY 2015 VL 6 IS 2 BP 486 EP 497 DI 10.3996/022015-JFWM-008 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CY8TT UT WOS:000366681900021 ER PT J AU Dyer, J Mercer, A Rigby, JR Grimes, A AF Dyer, Jamie Mercer, AndreW Rigby, James R. Grimes, Alexandria TI Identification of recharge zones in the Lower Mississippi River alluvial aquifer using high-resolution precipitation estimates SO JOURNAL OF HYDROLOGY LA English DT Article DE Precipitation; Lower Mississippi River alluvial aquifer; Recharge; Principal component analysis ID SMAR CONCEPTUAL-MODEL; GROUNDWATER RECHARGE; UNITED-STATES; RAINFALL; HYDROGRAPHS; FLUCTUATION; ALGORITHM AB Water resources in the lower Mississippi River alluvial valley play a critical role in agricultural productivity due to the widespread use of irrigation during the growing season. However, the unknown specifics of surface atmosphere feedbacks in the region, along with diminishing groundwater availability and the non-sustainable trend in irrigation draws from the alluvial aquifer, makes it difficult for water resource managers to make sound decisions for future water sustainability. As a result, it is crucial to identify spatial and temporal associations between local rainfall patterns and groundwater levels to determine the influence of precipitation on regional aquifer recharge. Specifically, it is critical to define the recharge zones of the aquifer so that rainfall distribution can be used to assess potential groundwater recovery. This project addresses the issue of defining areas of recharge in the lower Mississippi River alluvial aquifer (LMRAA) through an assessment of historical precipitation variability using high-resolution radar-derived precipitation estimates. A rotated principal component analysis (RPCA) of both groundwater and precipitation data from October through April is used to define locations where aquifer levels show the greatest variability, with a stepwise regression approach used to define areas where rainfall and groundwater levels show the strongest association. Results show that the greatest recharge through direct rainfall is along the Tallahatchie River basin in the northeastern Mississippi Delta, with recharge along the periphery of the LMRAA likely a result of direct water flux from surface hydrologic features. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dyer, Jamie; Mercer, AndreW; Grimes, Alexandria] Mississippi State Univ, Dept Geosci, Mississippi State, MS 39762 USA. [Rigby, James R.] ARS, USDA, Oxford, MS 38655 USA. RP Dyer, J (reprint author), Mississippi State Univ, Dept Geosci, 355 E Lee Blvd,108 Hilbun Hall, Mississippi State, MS 39762 USA. EM jamie.dyer@msstate.edu FU Mississippi Water Resources Research Institute [G11AP20088]; US Geological Survey [G11AP20088]; National Science Foundation FX The authors would like to thank the Mississippi Water Resources Research Institute and the US Geological Survey for funding to complete this research through award number G11AP20088. The authors would also like to thank the anonymous reviewers for their insightful comments and suggestions that helped to improve the final manuscript. The radar-based multi-sensor precipitation estimates used in this study were provided by NCAR/EOL under sponsorship of the National Science Foundation, and can be obtained free-of-charge at http://data.eol.ucar.edu. The groundwater data were obtained from the Yazoo Mississippi Delta Joint Water management District (http://www.ymd.org). NR 30 TC 0 Z9 0 U1 9 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD DEC PY 2015 VL 531 SI SI BP 360 EP 369 DI 10.1016/j.jhydrol.2015.07.016 PN 2 PG 10 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CZ0BF UT WOS:000366769400012 ER PT J AU Yen, H Su, YW Wolfe, JE Chen, ST Hsu, YC Tseng, WH Brady, DM Jeong, J Arnold, JG AF Yen, Haw Su, Yu-Wen Wolfe, June E., III Chen, Shien-Tsung Hsu, Yu-Chao Tseng, Wen-Hsiao Brady, Dawn M. Jeong, Jaehak Arnold, Jeffrey G. TI Assessment of input uncertainty by seasonally categorized latent variables using SWAT SO JOURNAL OF HYDROLOGY LA English DT Article DE Input uncertainty; Latent variables; Model calibration; IPEAT; SWAT ID WATER ASSESSMENT-TOOL; TRANSFERABILITY; CALIBRATION; MODELS; SOIL AB Watershed processes have been explored with sophisticated simulation models for the past few decades. It has been stated that uncertainty attributed to alternative sources such as model parameters, forcing inputs, and measured data should be incorporated during the simulation process. Among varying uncertainty sources, input uncertainty attributed to precipitation data exhibits a dominant role, as it is the source driving most hydrologically-related processes. In previous studies, latent variables (normally distributed random noise) have been implemented to explicitly incorporate input uncertainty from precipitation data. However, it may not be appropriate to apply the same set of latent variables throughout temporal series without considering seasonal effects. In this study, seasonally categorized latent variables were defined to investigate potential effects on model predictions and associated predictive uncertainty. Results show that the incorporation of seasonal latent variables resulted in better statistical solutions (NSE, Nash-Sutcliffe Efficiency coefficient) for both calibration (0.58([streamflow])/0.73([sediment])/0.59([ammonia])) and validation (0.57([streamflow])/0.45([sediment])/0.53([ammonia])) periods. Alternative definitions of Dry/Wet seasonality (two definitions are defined in this study) also affected model predictions. In addition, it was determined that predictive uncertainty can be enhanced by incorporating more latent variables during model calibration. The implementations of proposed seasonal latent variables have further substantiated the importance of incorporating seasonal effects when conducting comparable approaches. Applications of latent variables on future work should evaluate potential effects on model predictions before performing associated scientific studies or relevant decision making processes. (C) 2015 Elsevier B.V. All rights reserved. C1 [Yen, Haw; Wolfe, June E., III; Jeong, Jaehak] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Su, Yu-Wen; Hsu, Yu-Chao] Natl Cheng Kung Univ, Dept Hydraul & Ocean Engn, Tainan 70101, Taiwan. [Chen, Shien-Tsung] Feng Chia Univ, Dept Water Resources Engn & Conservat, Taichung 40724, Taiwan. [Tseng, Wen-Hsiao] Minist Econ Affairs, Water Resources Agcy, Water Resources Planning Inst, Taipei, Taiwan. [Brady, Dawn M.] Aqua Aerob Syst Inc, Loves Pk, IL 61111 USA. [Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Yen, H (reprint author), Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com RI Chen , Shien-Tsung/F-3552-2011 OI Chen , Shien-Tsung/0000-0002-6978-3985 FU United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components; Texas Commission on Environmental Quality [582-13-30048]; Ministry of Science and Technology in Taiwan, Republic of China [MOST 103-2811-M-006-050] FX This project was funded by grants from the United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components, and it was in part supported by Texas Commission on Environmental Quality (Award Number: 582-13-30048). The authors also appreciate for the continuous support from the Ministry of Science and Technology in Taiwan, Republic of China (MOST 103-2811-M-006-050). Please remember that USDA is an equal opportunity employer and provider! NR 35 TC 0 Z9 0 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD DEC PY 2015 VL 531 BP 685 EP 695 DI 10.1016/j.jhydrol.2015.10.058 PN 3 PG 11 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CZ0BJ UT WOS:000366769800014 ER PT J AU Fleming, GM Wunderle, JM Ewert, DN O'Brien, JJ Helmer, EH AF Fleming, Genie M. Wunderle, Joseph M., Jr. Ewert, David N. O'Brien, Joseph J. Helmer, Eileen H. TI Functional attributes of two subtropical shrubs and implications for the distribution and management of endangered bird habitat SO JOURNAL OF PLANT ECOLOGY LA English DT Article DE anthropogenic disturbance; black torch; plant functional type; vital attributes; wild sage ID TROPICAL DRY FOREST; KIRTLANDS-WARBLERS; PLANT-COMMUNITIES; CAPRA-HIRCUS; TRAITS; VEGETATION; BAHAMAS; ISLAND; ECOSYSTEMS; CLASSIFICATION AB The fruits of Erithalis fruticosa L. and Lantana involucrata L. are important in the diet of US federally endangered Kirtland's Warblers (Setophaga kirtlandii) wintering in the Bahamas archipelago. These two shrubs occur in tropical and subtropical dry forests, including forests that have been subjected to recent disturbance. Despite their importance to the endangered warbler, the disturbance ecology of these shrubs is poorly understood. We sought to determine, based on functional characteristics of the plants, whether their presence is favored by a particular type or regime of disturbance. We used data from field experiments (seed broadcasting and shrub cutting) conducted on the island of Eleuthera, The Bahamas to determine mechanisms of and conditions favoring establishment and persistence ('vital attributes') of E. fruticosa and L. involucrata, which enabled categorization according to the plant functional types defined by Noble and Slatyer (1980). We then compared hypothesized distributions of these plant functional types among different anthropogenic disturbance regimes to observed distributions of E. fruticosa and L. involucrata in order to identify disturbance regimes most likely to produce habitat used by Kirtland's Warblers. E. fruticosa and L. involucrata were functionally categorized as widely dispersed but largely shade intolerant species capable of establishing or regenerating individuals after disturbance via both seeds and vegetative mechanisms. Both hypothesized and observed distribution patterns indicated the shrubs were favored by a regime of frequent disturbance producing open canopy and ground layers. Among the anthropogenic disturbances we examined, areas of large-scale land clearing combined with subsequent goat grazing most often supported E. fruticosa and L. involucrata, while the shrubs were relatively rare in burned areas. Utilizing the plant functional type framework in combination with field data to evaluate predictions of species occurrence among different disturbances regimes provides a strong theoretical basis for conservation strategies. Understanding which disturbance types favor a habitat of concern and the mechanisms by which they do so can aid the prioritization of areas for protection or the design of habitat management protocols. C1 [Fleming, Genie M.] Puerto Rican Conservat Fdn, San Juan, PR 00936 USA. [Wunderle, Joseph M., Jr.; Helmer, Eileen H.] US Forest Serv, Int Inst Trop Forestry, USDA, Sabana Field Res Stn, Luquillo, PR 00773 USA. [Ewert, David N.] Nature Conservancy, Lansing, MI 48906 USA. [O'Brien, Joseph J.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Athens, GA 30602 USA. RP Fleming, GM (reprint author), Puerto Rican Conservat Fdn, POB 362495, San Juan, PR 00936 USA. EM fleming.gm@gmail.com FU International Programs of the US Department of Agriculture Forest Service; Nature Conservancy; Puerto Rican Conservation Foundation FX International Programs of the US Department of Agriculture Forest Service with grants to The Nature Conservancy and the Puerto Rican Conservation Foundation. NR 50 TC 1 Z9 1 U1 3 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1752-9921 EI 1752-993X J9 J PLANT ECOL JI J. Plant Ecol. PD DEC PY 2015 VL 8 IS 6 BP 578 EP 592 DI 10.1093/jpe/rtu036 PG 15 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA CY4PL UT WOS:000366390400003 ER PT J AU Engelhardt, BM Chambers, JC Weisberg, PJ AF Engelhardt, Blake M. Chambers, Jeanne C. Weisberg, Peter J. TI Geomorphic predictors of riparian vegetation in small mountain watersheds SO JOURNAL OF PLANT ECOLOGY LA English DT Article DE Great Basin; multiscale; plant community analysis; process zone; riparian ID BEGA CATCHMENT; RIVER; LANDSCAPE; BASIN; USA; DISTURBANCE; SCALE; TRANSVERSE; CALIFORNIA; AUSTRALIA AB Hydrogeomorphic processes operating at watershed, process zone and site scales influence the distribution of riparian vegetation. However, most studies examining the relationships between hydrogeomorphic processes and riparian vegetation are conducted at site scales. We quantified the relative importance of watershed, process zone and site geomorphic characteristics for predicting riparian plant community types and plant species abundances in four small mountain watersheds in central Nevada, USA. We mapped riparian vegetation types and identified process zones (based on dominant geomorphic process and valley fill material) within the watersheds. We sampled sites in each combination of vegetation type and process zone (n = 184 sites) and collected data on watershed scale factors, valley and stream geomorphic characteristics and on plant cover of each geomorphic surface. Plant community types were defined by cluster and indicator species analyses of plant cover data, and related to geomorphic variables using ordination analysis (nonmetric multidimensional scaling). Linear mixed effects models were used to predict abundances of indicator species. Variables describing position in the watershed (elevation, contributing area) that are related to gradients of temperature, moisture and stream discharge were of primary importance in predicting plant community types. Variables describing local geomorphic setting (valley width, stream gradient, channel sediments, geomorphic surface height) were of secondary importance, but accurately described the geomorphic setting of indicator species. The process zone classification did not include position in the watershed or channel characteristics and only predicted plant community types with unique geomorphic settings. In small mountain watersheds, predicting riparian vegetation distribution requires explicit consideration of scale and geomorphic context within and among watersheds in addition to site variables. C1 [Engelhardt, Blake M.; Weisberg, Peter J.] Univ Nevada, Nat Resources & Environm Sci Dept, Reno, NV 89557 USA. [Chambers, Jeanne C.] US Forest Serv, USDA, Rocky Mt Res Stn, Reno, NV 89512 USA. RP Weisberg, PJ (reprint author), Univ Nevada, Nat Resources & Environm Sci Dept, MS 186,1664 North Virginia St, Reno, NV 89557 USA. EM pweisberg@cabnr.unr.edu FU USDA Forest Service, Rocky Mountain Research Station [08-JV-11221632-196] FX USDA Forest Service, Rocky Mountain Research Station (Agreement Number: 08-JV-11221632-196). NR 45 TC 0 Z9 0 U1 5 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1752-9921 EI 1752-993X J9 J PLANT ECOL JI J. Plant Ecol. PD DEC PY 2015 VL 8 IS 6 BP 593 EP 604 DI 10.1093/jpe/rtv002 PG 12 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA CY4PL UT WOS:000366390400004 ER PT J AU Maccabe, AT Crawford, L Heider, LE Hooper, B Mann, CJ Pappaioanou, M AF Maccabe, Andrew T. Crawford, Lester Heider, Lawrence E. Hooper, Billy Mann, Curt J. Pappaioanou, Marguerite TI Association of American Veterinary Medical Colleges (AAVMC): 50 Years of History and Service SO JOURNAL OF VETERINARY MEDICAL EDUCATION LA English DT Article DE Association of American Veterinary Medical Colleges (AAVMC); federal investment in veterinary medical education; diversity in veterinary medicine; Veterinary Medical College Application Service (VMCAS); Veterinary Medical Caucus; veterinary medical workforce AB The mission of the Association of American Veterinary Medical Colleges (AAVMC) is to advance the quality of academic veterinary medicine. Founded in 1966 by the 18 US colleges of veterinary medicine and 3 Canadian colleges of veterinary medicine then in existence, the AAVMC is celebrating 50 years of public service. Initially, the AAVMC comprised the Council of Deans, the Council of Educators, and the Council of Chairs. In 1984, the tri-cameral structure was abandoned and a new governing structure with a board of directors was created. In 1997, the AAVMC was incorporated in Washington, DC and a common application service was created. Matters such as workforce issues and the cost of veterinary medical education have persisted for decades. The AAVMC is a champion of diversity in the veterinary profession and a strong advocate for One Health. The AAVMC has adopted a global perspective as more international colleges of veterinary medicine have earned COE accreditation and become members. C1 [Maccabe, Andrew T.] Assoc Amer Vet Med Coll, Washington, DC 20005 USA. [Crawford, Lester] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Crawford, Lester] Georgetown Univ, Washington, DC 20057 USA. [Crawford, Lester] Univ Georgia, Athens, GA 30602 USA. [Heider, Lawrence E.] Ohio State Univ, Columbus, OH 43210 USA. [Heider, Lawrence E.] Univ Prince Edward Isl, Charlottetown, PE C1A 4P3, Canada. [Hooper, Billy] Amer Assoc Vet Pathologists, New York, NY USA. [Mann, Curt J.] USDA, Washington, DC USA. RP Maccabe, AT (reprint author), Assoc Amer Vet Med Coll, Washington, DC 20005 USA. EM amaccabe@aavmc.org; crawfordlm@aol.com; heider.1@osu.edu; bill1hooper@gmail.com; cjm@empryse.net; pappam@comcast.net NR 7 TC 0 Z9 0 U1 2 U2 3 PU UNIV TORONTO PRESS INC PI TORONTO PA JOURNALS DIVISION, 5201 DUFFERIN ST, DOWNSVIEW, TORONTO, ON M3H 5T8, CANADA SN 0748-321X EI 1943-7218 J9 J VET MED EDUC JI J. Vet. Med. Educ. PD WIN PY 2015 VL 42 IS 5 BP 395 EP 402 DI 10.3138/jvme.0615-089R PG 8 WC Education, Scientific Disciplines; Veterinary Sciences SC Education & Educational Research; Veterinary Sciences GA CY8ND UT WOS:000366664600002 PM 26673207 ER PT J AU Roberts, R Zhang, JC Mayberry, LK Tatineni, S Browning, KS Rakotondrafara, AM AF Roberts, Robyn Zhang, Jincan Mayberry, Laura K. Tatineni, Satyanarayana Browning, Karen S. Rakotondrafara, Aurelie M. TI A Unique 5 ' Translation Element Discovered in Triticum Mosaic Virus SO JOURNAL OF VIROLOGY LA English DT Article ID CAP-INDEPENDENT TRANSLATION; TOBACCO ETCH VIRUS; RIBOSOME ENTRY SITE; PLANT VIRAL RNAS; ENCEPHALOMYOCARDITIS VIRUS; MESSENGER-RNAS; INTERNAL INITIATION; POLY(A) TAIL; SECONDARY STRUCTURE; FAMILY POTYVIRIDAE AB Several plant viruses encode elements at the 5' end of their RNAs, which, unlike most cellular mRNAs, can initiate translation in the absence of a 5' m7GpppG cap. Here, we describe an exceptionally long (739-nucleotide [nt]) leader sequence in triticum mosaic virus (TriMV), a recently emerged wheat pathogen that belongs to the Potyviridae family of positive-strand RNA viruses. We demonstrate that the TriMV 5' leader drives strong cap-independent translation in both wheat germ extract and oat protoplasts through a novel, noncanonical translation mechanism. Translation preferentially initiates at the 13th start codon within the leader sequence independently of eIF4E but involves eIF4G. We truncated the 5' leader to a 300-nucleotide sequence that drives cap-independent translation from the 5' end. We show that within this sequence, translation activity relies on a stem-loop structure identified at nucleotide positions 469 to 490. The disruption of the stem significantly impairs the function of the 5' untranslated region (UTR) in driving translation and competing against a capped RNA. Additionally, the TriMV 5' UTR can direct translation from an internal position of a bicistronic mRNA, and unlike cap-driven translation, it is unimpaired when the 5' end is blocked by a strong hairpin in a monocistronic reporter. However, the disruption of the identified stem structure eliminates such a translational advantage. Our results reveal a potent and uniquely controlled translation enhancer that may provide new insights into mechanisms of plant virus translational regulation. IMPORTANCE Many members of the Potyviridae family rely on their 5' end for translation. Here, we show that the 739-nucleotide-long triticum mosaic virus 5' leader bears a powerful translation element with features distinct from those described for other plant viruses. Despite the presence of 12 AUG start codons within the TriMV 5' UTR, translation initiates primarily at the 13th AUG codon. The TriMV 5' UTR is capable of driving cap-independent translation in vitro and in vivo, is independent of eIF4E, and can drive internal translation initiation. A hairpin structure at nucleotide positions 469 to 490 is required for the cap-independent translation and internal translation initiation abilities of the element and plays a role in the ability of the TriMV UTR to compete against a capped RNA in vitro. Our results reveal a novel translation enhancer that may provide new insights into the large diversity of plant virus translation mechanisms. C1 [Roberts, Robyn; Zhang, Jincan; Tatineni, Satyanarayana; Rakotondrafara, Aurelie M.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Tatineni, Satyanarayana] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Mayberry, Laura K.; Browning, Karen S.] Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA. RP Rakotondrafara, AM (reprint author), Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. EM rakotondrafa@wisc.edu FU HATCH-ACT Formula funds [WIS01654, WIS01796]; National Science Foundation [MCB1052530, Arabidopsis 2010 S-0000335] FX This work was supported by HATCH-ACT Formula funds (WIS01654 and WIS01796 to A.R.) and by the National Science Foundation (MCB1052530 and Arabidopsis 2010 S-0000335 to K.S.B.). NR 53 TC 1 Z9 1 U1 1 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD DEC PY 2015 VL 89 IS 24 BP 12427 EP 12440 DI 10.1128/JVI.02099-15 PG 14 WC Virology SC Virology GA CZ1WS UT WOS:000366897200019 PM 26423954 ER PT J AU Whitcomb, EA Chiu, CJ Taylor, A AF Whitcomb, Elizabeth A. Chiu, Chung-Jung Taylor, Allen TI Dietary glycemia as a determinant of health and longevity SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE Glycemic index; Glycation; Ubiquitin; Proteasome; Autophagy; Eye disease ID UBIQUITIN-PROTEASOME PATHWAY; MACULAR DEGENERATION; CALORIC RESTRICTION; EYE DISEASE; METABOLIC SYNDROME; DAMAGED PROTEINS; RHESUS-MONKEYS; LIFE-SPAN; INDEX; CARBOHYDRATE AB The role of diet in extending lifespan and healthspan has been the subject of much research and debate. Our recent epidemiological and in vivo data suggest that carbohydrate quality can be a major determinant in prolonging eye health. Additionally, excessive carbohydrate intake can contribute to the exacerbation of many different diseases. The metabolic diversity of the tissues that are affected by excessive carbohydrate intake suggests that dietary carbohydrate quality may affect cellular homeostasis. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Whitcomb, Elizabeth A.; Chiu, Chung-Jung; Taylor, Allen] Tufts Univ, USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. RP Taylor, A (reprint author), Tufts Univ, USDA Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. EM allen.taylor@tufts.edu FU U.S. Department of Agriculture - Agricultural Research Service (ARS) [58-1950-4-003]; National Eye Institute [EY-13250, EY-21212] FX This material is based on work supported by the U.S. Department of Agriculture - Agricultural Research Service (ARS), under Agreement No. 58-1950-4-003. Additional funding from the National Eye Institute to AT EY-13250, EY-21212. We also thank Dr. Sheldon Rowan for work on Figure 2. NR 52 TC 2 Z9 3 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 EI 1872-9452 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD DEC PY 2015 VL 46 BP 14 EP 20 DI 10.1016/j.mam.2015.08.005 PG 7 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA CY6QP UT WOS:000366534200004 PM 26282832 ER PT J AU Murdiyarso, D Purbopuspito, J Kauffman, JB Warren, MW Sasmito, SD Donato, DC Manuri, S Krisnawati, H Taberima, S Kurnianto, S AF Murdiyarso, Daniel Purbopuspito, Joko Kauffman, J. Boone Warren, Matthew W. Sasmito, Sigit D. Donato, Daniel C. Manuri, Solichin Krisnawati, Haruni Taberima, Sartji Kurnianto, Sofyan TI The potential of Indonesian mangrove forests for global climate change mitigation SO NATURE CLIMATE CHANGE LA English DT Article ID BLUE CARBON; LAND-USE; PHILIPPINES; EMISSIONS; BIOMASS AB Mangroves provide a wide range of ecosystem services, including nutrient cycling, soil formation, wood production, fish spawning grounds, ecotourism and carbon (C) storage(1). High rates of tree and plant growth, coupled with anaerobic, water-logged soils that slow decomposition, result in large long-term C storage. Given their global significance as large sinks of C, preventing mangrove loss would be an effective climate change adaptation and mitigation strategy. It has been reported that C stocks in the Indo-Pacific region contain on average 1,023MgC ha(-1) (ref. 2). Here, we estimate that Indonesian mangrove C stocks are 1,083 +/- 378MgC ha(-1). Scaled up to the country-level mangrove extent of 2.9 Mha (ref. 3), Indonesia's mangroves contained on average 3.14 PgC. In three decades Indonesia has lost 40% of its mangroves(4), mainly as a result of aquaculture development(5). This has resulted in annual emissions of 0.07-0.21 Pg CO(2)e. Annual mangrove deforestation in Indonesia is only 6% of its total forest loss(6); however, if this were halted, total emissions would be reduced by an amount equal to 10-31% of estimated annual emissions from land-use sectors at present. Conservation of carbon-rich mangroves in the Indonesian archipelago should be a high-priority component of strategies to mitigate climate change. C1 [Murdiyarso, Daniel; Purbopuspito, Joko; Sasmito, Sigit D.; Kurnianto, Sofyan] Ctr Int Forestry Res CIFOR, Bogor 16115, Indonesia. [Murdiyarso, Daniel] Bogor Agr Univ, Dept Geophys & Meteorol, Bogor 16680, Indonesia. [Purbopuspito, Joko] Sam Ratulangi Univ, Dept Soil Sci, Manado 95115, Indonesia. [Kauffman, J. Boone; Kurnianto, Sofyan] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Warren, Matthew W.] US Forest Serv, USDA, Durham, NH 03824 USA. [Donato, Daniel C.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Manuri, Solichin] Australian Natl Univ, Fenner Sch Environm & Soc, Canberra, ACT 0200, Australia. [Krisnawati, Haruni] Forestry Res & Dev Agcy, Res & Dev Ctr Conservat & Rehabil, Bogor 16610, Indonesia. [Taberima, Sartji] Univ Papua New Guinea, Manokwari 98314, Indonesia. RP Murdiyarso, D (reprint author), Ctr Int Forestry Res CIFOR, Bogor 16115, Indonesia. EM d.murdiyarso@cgiar.org RI Sasmito, Sigit D./E-8715-2013; Purbopuspito, Joko/P-1165-2015 OI Sasmito, Sigit D./0000-0001-5864-8596; Purbopuspito, Joko/0000-0002-4631-596X FU US Agency for International Development (USAID) FX This work is supported by the US Agency for International Development (USAID). D. Sheil provided useful comments on an earlier draft. We are also grateful to officials from Sembilang, Bunaken and Tanjung Puting National Parks for their assistance, without which the field work could have not been performed. We would also like to thank the many technicians, students and villagers who assisted in data collection in the field. NR 29 TC 25 Z9 25 U1 22 U2 77 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD DEC PY 2015 VL 5 IS 12 BP 1089 EP 1092 DI 10.1038/NCLIMATE2734 PG 4 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CY4UA UT WOS:000366402900019 ER PT J AU Potter, TL Olson, DM Ni, XZ Rains, GC AF Potter, Thomas L. Olson, Dawn M. Ni, Xinzhi Rains, Glen C. TI A re-examination of corn (Zea mays L.) ear volatiles SO PHYTOCHEMISTRY LETTERS LA English DT Article DE SPME; Volatiles; Alcohol acetates; Corn earworm ID HOST-PLANT; EARWORM LEPIDOPTERA; WORM ATTRACTANTS; LEAF VOLATILES; HELIOTHIS; BEHAVIOR; MAIZE; IDENTIFICATION; CONSTITUENTS; OVIPOSITION AB Helicoverpa zea (Boddie) is a major insect pest of corn and other agricultural crops. An improved understanding of semiochemcials that control adult behavior is needed to develop alternative control measures. In this study, overnight SPME collection of volatiles from corn ears enclosed in Teflon bags in the field at two stages of development were made. C-8-C-10 aldehydes, a C-8-alcohol, C-6-C-9 alcohol acetates, and numerous monoterpenes, sesquiterpenes, sequiterpene alcohols, and geosmin were identified after thermal desorption and GC/MS. Structural assignments of the alcohol acetates, Z-3-hexenyl acetate, 2-heptyl acetate, 2-nonyl acetate, and 4-nonyl acetate, the monoterpenes, alpha- and beta-ocimene, and geosmin were made by analysis of standards that were purchased or prepared in the laboratory. All other assignments were based on published Kovat's retention time indices (KI) and mass spectra. Pair-wise comparison of the relative amounts of each component between two groups of corn ears defined by silk weight did not identify significant differences, thus it is unknown whether or not silk weight impacted volatile emission composition and rate. To our knowledge three compounds detected in SPME collections, 2-heptyl acetate, 2-nonyl acetate, and 4-nonyl acetate have not been previously reported in corn ear or silk volatiles. Their impact on the flight response of gravid earworm females was evaluated in a flight chamber. No significant response to the individual compounds or a blend of all three was observed. Thus, their impact on moth behavior remains uncertain. Published by Elsevier B.V. on behalf of Phytochemical Society of Europe. C1 [Potter, Thomas L.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. [Olson, Dawn M.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Ni, Xinzhi] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Rains, Glen C.] Univ Georgia, Dept Entomol, Tifton, GA USA. RP Potter, TL (reprint author), USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. EM tom.potter@ars.usda.gov OI Potter, Thomas/0000-0003-2663-0054 FU USDA Agricultural Research Service; University of Georgia Coastal Plain Experiment Station, Tifton, GA FX J. C. Mullis, P. M. Tapp, and L. Walters (USDA-ARS, Tifton), and B. Berry and J. Sparks (University of Georgia, Tifton) maintained experimental plots. The USDA Agricultural Research Service and the University of Georgia Coastal Plain Experiment Station, Tifton, GA provided all financial support. NR 37 TC 0 Z9 0 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3900 EI 1876-7486 J9 PHYTOCHEM LETT JI Phytochem. Lett. PD DEC PY 2015 VL 14 BP 280 EP 286 DI 10.1016/j.phytol.2015.10.026 PG 7 WC Plant Sciences; Oncology SC Plant Sciences; Oncology GA CY7DV UT WOS:000366569300049 ER PT J AU Peebles, ED Jacob, R Branton, SL Evans, JD Leigh, SA Gerard, PD AF Peebles, E. D. Jacob, R. Branton, S. L. Evans, J. D. Leigh, S. A. Gerard, P. D. TI Effects of different vaccine combinations against Mycoplasma gallisepticum on the digestive and reproductive organ characteristics of commercial egg-laying hens SO POULTRY SCIENCE LA English DT Article DE bacterin; commercial layer; digestive and reproductive organs; Mycoplasma gallisepticum; vaccine ID LAYER CHICKENS; BLOOD CHARACTERISTICS; INOCULATION OVERLAYS; QUALITY PARAMETERS; SIZE DISTRIBUTION; AVIAN MYCOPLASMA; DUAL INFECTION; STRAIN; AGE; PERFORMANCE AB Mycoplasma gallisepticum (MG) is a major and economically significant pathogen of avian species. When administered before lay, F-strain MG (FMG) can reduce egg production during lay, but the ts-11 strain of MG (ts11MG) does not exert this effect. Two trials were conducted to determine the effects of pre-lay vaccinations of ts11MG, MG-Bacterin (MGBac), or their combination, in conjunction with an FMG vaccination overlay after peak production on the digestive and reproductive organ characteristics of Hy-Line W-36 layers housed in biological isolation units (4 units per treatment, 10 birds per unit). The following vaccination treatments were administered at 10 wk of age (woa): 1) Control (no vaccinations); 2) MG-Bac; 3) ts11MG; and 4) ts11MG and MGBac combination (ts11MG+MGBac). At 45 woa, half of the birds were vaccinated with a laboratory stock of high passage FMG. In both trials, parameters determined in 4 birds per unit at 55 woa included: BW; fatty liver hemorrhagic syndrome incidence; mean number of mature ovarian follicles; ovarian, oviduct, and small intestine weights; and the weights and lengths of the various portions of the oviduct and small intestine. Treatment effects were observed for the weights of the entire small intestine and the duodenum, jejunum, and ileum, as percentages of BW; and for vagina weight as a percentage of total oviduct weight. In general, the weights of the small intestine and its 3 components were increased in response to the FMG vaccine that was administered at 45 woa. An FMG vaccination at 45 woa may increase relative intestine weight in layers; however, use of a prelay MGBac vaccine alone or in combination with ts11MG, with or without an FMG overlay, does not affect the gross characteristics of their digestive and reproductive organs, and may be used without having an adverse effect on their performance, as was observed in a previous companion study. C1 [Peebles, E. D.; Jacob, R.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. [Branton, S. L.; Leigh, S. A.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. [Evans, J. D.; Gerard, P. D.] Clemson Univ, Dept Math Sci, Clemson, SC 29634 USA. RP Peebles, ED (reprint author), Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. EM d.peebles@msstate.edu FU United States Department of Agriculture (USDA) [58-6406-4-016] FX This work was funded by grant No. 58-6406-4-016 from the United States Department of Agriculture (USDA). NR 36 TC 1 Z9 1 U1 1 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD DEC PY 2015 VL 94 IS 12 BP 2898 EP 2904 DI 10.3382/ps/pev269 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY9VJ UT WOS:000366754100006 PM 26467015 ER PT J AU Park, SH Dowd, SE McReynolds, JL Byrd, JA Nisbet, DJ Ricke, SC AF Park, Si Hong Dowd, Scot E. McReynolds, Jack L. Byrd, James A. Nisbet, David J. Ricke, Steven C. TI Evaluation of feed grade sodium bisulfate impact on gastrointestinal tract microbiota ecology in broilers via a pyrosequencing platform SO POULTRY SCIENCE LA English DT Article DE sodium bisulfate; microbiota; pyrosequencing; broiler chicken ID BACTERIAL DIVERSITY; PROBIOTIC BACTERIA; SPECIES RICHNESS; PROPIONIC-ACID; CHICKENS; ILEUM; CECUM; LACTOBACILLUS; COMMUNITY; CROP AB The gastrointestinal microbial community in broiler chickens consists of many different species of bacteria, and the overall microbiota can vary from bird to bird. To control pathogenic bacteria in broilers and improve gut health, numerous potential dietary amendments have been used. In this study, we used a pyrosequencing platform to evaluate the effect of sodium bisulfate on microbiota of the crop, cecum, and ileum of broiler chickens grown over several weeks. The diversity information in each digestive organ sample exhibited considerable variation and was clearly separable, suggesting distinct bacterial populations. Although no apparent microbial clustering occurred between the control and the dietary treatments, we did observe shifts in overall microbiota populations in the crop, ileum, and ceca as well as changes in specific microorganisms such as Bacteroides, Clostridium, and Lactobacillus species that were identified as birds became older. C1 [Park, Si Hong; Ricke, Steven C.] Univ Arkansas, Dept Food Sci, Ctr Food Safety, Fayetteville, AR 72703 USA. [Dowd, Scot E.] MR DNA Mol Res LP, Shallowater, TX USA. [McReynolds, Jack L.; Byrd, James A.; Nisbet, David J.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX USA. RP Ricke, SC (reprint author), Univ Arkansas, Dept Food Sci, Ctr Food Safety, Fayetteville, AR 72703 USA. EM sricke@uark.edu FU Jones-Hamilton Co. (Walbridge, OH) FX Jones-Hamilton Co. (Walbridge, OH) supported Scot E. Dowd's research on this project. NR 43 TC 0 Z9 0 U1 2 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD DEC PY 2015 VL 94 IS 12 BP 3040 EP 3047 DI 10.3382/ps/pev274 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY9VJ UT WOS:000366754100023 PM 26467017 ER PT J AU Berrang, ME Cosby, DE Cox, NA Cason, JA Richardson, KE AF Berrang, M. E. Cosby, D. E. Cox, N. A. Cason, J. A. Richardson, K. E. TI Optimizing buffering chemistry to maintain near neutral pH of broiler feed during pre-enrichment for Salmonella SO POULTRY SCIENCE LA English DT Article DE broiler feed; buffer; pH; pre-enrichment; Salmonella ID POULTRY FEEDS; ENTEROBACTERIACEAE; RECOVERY; MEDIA AB Salmonella is a human pathogen that can accompany live broilers to the slaughter plant, contaminating fully processed carcasses. Feed is one potential source of Salmonella to growing broilers. Monitoring feed for the presence of Salmonella is part of good agricultural practice. The first step in culturing feed for Salmonella (which may be at low numbers and sub-lethally stressed) is to add it to a pre-enrichment broth which is incubated for 24 h. During the course of pre-enrichment, extraneous bacteria metabolize carbohydrates in some feed and excrete acidic byproducts, causing the pH to drop dramatically. An acidic pre-enrichment pH can injure or kill Salmonella resulting in a failure to detect, even if it is present and available to infect chickens. The objective of this study was to test an array of buffering chemistries to prevent formation of an injurious acidic environment during pre-enrichment of feed in peptone water. Five grams of feed were added to 45 mL of peptone water buffered with carbonate, Tris pH 8, and phosphate buffering ingredients individually and in combination. Feed was subjected to a pre-enrichment at 35 degrees C for 24 h; pH was measured at 0, 18, and 24 h. Standard phosphate buffering ingredients at concentrations up to 4 times the normal formulation were unable to fully prevent acidic conditions. Likewise, carbonate and Tris pH 8 were not fully effective. The combination of phosphate, carbonate, and Tris pH 8 was the most effective buffer tested. It is recommended that a highly buffered pre-enrichment broth be used to examine feed for the presence of Salmonella. C1 [Berrang, M. E.; Cosby, D. E.; Cox, N. A.] ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30605 USA. [Richardson, K. E.] Anitox Corp, Lawrenceville, GA 30043 USA. RP Berrang, ME (reprint author), ARS, USDA, US Natl Poultry Res Ctr, Athens, GA 30605 USA. EM mark.berrang@ars.usda.gov NR 11 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD DEC PY 2015 VL 94 IS 12 BP 3048 EP 3051 DI 10.3382/ps/pev277 PG 4 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY9VJ UT WOS:000366754100024 PM 26467004 ER PT J AU Wang, L Elliott, B Jin, XX Zeng, L Chen, J AF Wang, Lei Elliott, Brad Jin, Xixuan Zeng, Ling Chen, Jian TI Antimicrobial properties of nest volatiles in red imported fire ants, Solenopsis invicta (hymenoptera: formicidae) SO SCIENCE OF NATURE LA English DT Article DE Antimicrobial volatiles; Beauveria bassiana; Germination; Social immunity ID IMMUNITY; COLONIES; VENOM AB The antimicrobial property of volatiles produced by red imported fire ants, Solenopsis invicta, against Beauveria bassiana, a common entomopathogenic fungus, was demonstrated. The germination rate of B. bassiana spores was significantly reduced after they were exposed to volatiles within an artificial ant nest. Since the air that contained the same level of O-2 and CO2 as that in artificial fire ant nests did not suppress the germination rate of B. bassiana, the observed reduction of germination rate must be caused by the toxicity of nest volatiles. Nest fumigation may be an important component of the social immune system in S. invicta. C1 [Wang, Lei; Zeng, Ling] South China Agr Univ, Red Imported Fire Ant Res Ctr, Guangzhou 510642, Guangdong, Peoples R China. [Elliott, Brad; Jin, Xixuan; Chen, Jian] ARS, Natl Biol Control Lab, Southeast Area, USDA, Stoneville, MS 38776 USA. RP Chen, J (reprint author), ARS, Natl Biol Control Lab, Southeast Area, USDA, 59 Lee Rd, Stoneville, MS 38776 USA. EM jian.chen@ars.usda.gov NR 20 TC 0 Z9 0 U1 4 U2 16 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0028-1042 EI 1432-1904 J9 SCI NAT-HEIDELBERG JI Sci. Nat. PD DEC PY 2015 VL 102 IS 11-12 AR 66 DI 10.1007/s00114-015-1316-1 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CZ0WZ UT WOS:000366828400001 ER PT J AU Lee, K Lam, KH Kruel, AM Mahrhold, S Perry, K Cheng, LW Rummel, A Jin, RS AF Lee, Kwangkook Lam, Kwok-Ho Kruel, Anna-Magdalena Mahrhold, Stefan Perry, Kay Cheng, Luisa W. Rummel, Andreas Jin, Rongsheng TI Inhibiting oral intoxication of botulinum neurotoxin A complex by carbohydrate receptor mimics SO TOXICON LA English DT Article; Proceedings Paper CT TOXINS 2015 Basic Science and Clinical Aspects of Botulinum and Other Neurotoxins CY JAN 14-17, 2015 CL Lisbon, PORTUGAL SP Intl Neurotoxin Assoc (INA) DE Botulinum neurotoxin; Hemagglutinin; Progenitor toxin complex; Lactulose; Carbohydrate receptor; Inhibitor ID PROGENITOR TOXIN COMPLEX; CRYSTAL-STRUCTURE; E-CADHERIN; BINDING; PURIFICATION; TOXICITIES; AGENTS; CELLS; HOST AB Botulinum neurotoxins (BoNTs) cause the disease botulism manifested by flaccid paralysis that could be fatal to humans and animals. Oral ingestion of the toxin with contaminated food is one of the most common routes for botulism. BoNT assembles with several auxiliary proteins to survive in the gastrointestinal tract and is subsequently transported through the intestinal epithelium into the general circulation. Several hemagglutinin proteins form a multi-protein complex (HA complex) that recognizes host glycans on the intestinal epithelial cell surface to facilitate BoNT absorption. Blocking carbohydrate binding to the HA complex could significantly inhibit the oral toxicity of BoNT. Here, we identify lactulose, a galactose-containing non-digestible sugar commonly used to treat constipation, as a prototype inhibitor against oral BoNT/A intoxication. As revealed by a crystal structure, lactulose binds to the HA complex at the same site where the host galactose-containing carbohydrate receptors bind. In vitro assays using intestinal Caco-2 cells demonstrated that lactulose inhibits HA from compromising the integrity of the epithelial cell monolayers and blocks the internalization of HA. Furthermore, co-administration of lactulose significantly protected mice against BoNT/A oral intoxication in vivo. Taken together, these data encourage the development of carbohydrate receptor mimics as a therapeutic intervention to prevent BoNT oral intoxication. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Lee, Kwangkook; Lam, Kwok-Ho; Jin, Rongsheng] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA. [Kruel, Anna-Magdalena; Mahrhold, Stefan; Rummel, Andreas] Hannover Med Sch, Inst Toxikol, D-30623 Hannover, Germany. [Perry, Kay] Cornell Univ, Argonne Natl Lab, NE CAT, Argonne, IL 60439 USA. [Perry, Kay] Cornell Univ, Argonne Natl Lab, Dept Chem & Chem Biol, Argonne, IL 60439 USA. [Cheng, Luisa W.] ARS, Foodbome Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Rummel, A (reprint author), Hannover Med Sch, Inst Toxikol, D-30623 Hannover, Germany. EM rummel.andreas@mh-hannover.de; r.jin@uci.edu OI Jin, Rongsheng/0000-0003-0348-7363 FU National Institute of Allergy and Infectious Diseases (NIAID) [R01AI091823]; Swiss Federal Office for Civil Protection BABS [353003325]; United States Department of Agriculture, Agricultural Research Service, National Program project [NP108, CRIS 5325-42000-048-00D]; National Institute of General Medical Sciences [P41 GM103403]; U.S. DOE [DE-AC02-06CH11357] FX This work was partly supported by National Institute of Allergy and Infectious Diseases (NIAID) grant R01AI091823 to R.J. and by the Swiss Federal Office for Civil Protection BABS #353003325 to A.R.; L.W.C. was funded by the United States Department of Agriculture, Agricultural Research Service, National Program project NP108, CRIS 5325-42000-048-00D. NECAT at the Advanced Photon Source (APS) is supported by a grant from the National Institute of General Medical Sciences (P41 GM103403). Use of the APS, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory, was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357. The atomic coordinates and structure factors of the HA17-HA33-lactulose and HA17-HA33-IPTG complexes have been deposited in the Protein Data Bank under the accession codes 5BQU and 5BP5, respectively. NR 37 TC 1 Z9 1 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD DEC 1 PY 2015 VL 107 SI SI BP 43 EP 49 DI 10.1016/j.toxicon.2015.08.003 PN A PG 7 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA CY6SP UT WOS:000366539400007 PM 26272706 ER PT J AU Jaheer, M Chopra, R Kunder, KR Bhat, D Rashmi, KV Sathyanarayana, N AF Jaheer, M. Chopra, R. Kunder, K. R. Bhat, Deepika Rashmi, K. V. Sathyanarayana, N. TI Cytogenetic and ITS-psbA-trnH Sequence Analysis for Phylogenetic Inference in Mucuna sp of India SO TROPICAL PLANT BIOLOGY LA English DT Article DE Mucuna sp.; Karyomorphological analysis; Taxonomy; Phylogeny; nrITS; cp-psbA-trnH ID CHLOROPLAST-DNA; RETENTION INDEX; INVERTED REPEAT; LEGUMINOSAE; EVOLUTION; REGIONS; PLANT AB This paper reports new chromosomal information and earliest proof on efficacy of nrITS and cp-psbA-trnH gene sequences for barcoding and phylogenetic studies in Mucuna sp. First cytological evidence on M. sempervirens and M. bracteata revealed symmetrical karyotype in both the species. Meiotic anomalies evident from the formation of chromosomal laggards and bridges in M. gigantea and M. atropurpurea and unreduced pollens in M. bracteata suggest evolutionary events that might have shaped annual species in this genus. Molecular investigations using nrITS and cp-psbA-trnH sequences revealed them to be phylogenetically informative. Vis-a-vis individual genes, combined sequences of ITS and psbA-trnH sequences offered reliable data for species delineation. The results summarized here are expected to galvanize molecular taxonomic studies and open-up newer means for answering phylogenetic questions in this genus. C1 [Jaheer, M.; Bhat, Deepika; Rashmi, K. V.] Sir M Visvesvaraya Inst Technol, Dept Biotechnol, Bangalore, Karnataka, India. [Chopra, R.] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Kunder, K. R.] Rubicon Genom Inc, Ann Arbor, MI USA. [Sathyanarayana, N.] Sikkim Univ, Dept Bot, Tadong 737102, Gangtok, India. RP Sathyanarayana, N (reprint author), Sikkim Univ, Dept Bot, 6th Mile, Tadong 737102, Gangtok, India. EM nsathyanarayana@cus.ac.in OI CHOPRA, RATAN/0000-0003-2088-3341 FU Department of Science and Technology (DST), GOI [SR/SO/PS/0028/2011]; Dept. of Biotechnology (DBT), GOI [BT/PR3489/PBD/16/945/2011]; Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore; Sikkim University, Gangtok FX The authors acknowledge the financial support from; 1. Department of Science and Technology (DST), GOI; Grant number: SR/SO/PS/0028/2011.; 2. Dept. of Biotechnology (DBT), GOI. Grant number: BT/PR3489/PBD/16/945/2011; 3. Sri Krishnadevaraya Educational Trust (Sri KET), Bangalore,; 4. Sikkim University, Gangtok. NR 41 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-9756 EI 1935-9764 J9 TROP PLANT BIOL JI Trop. Plant Biol. PD DEC PY 2015 VL 8 IS 3-4 BP 108 EP 116 DI 10.1007/s12042-015-9154-z PG 9 WC Plant Sciences SC Plant Sciences GA CY4IS UT WOS:000366372400006 ER PT J AU Silverstein, J Cheng, PY Ruedy, KJ Kollman, C Beck, RW Klingensmith, GJ Wood, JR Willi, S Bacha, F Lee, J Cengiz, E Redondo, MJ Tamborlane, WV AF Silverstein, Janet Cheng, Peiyao Ruedy, Katrina J. Kollman, Craig Beck, Roy W. Klingensmith, Georgeanna J. Wood, Jamie R. Willi, Steven Bacha, Fida Lee, Joyce Cengiz, Eda Redondo, Maria J. Tamborlane, William V. CA Pediat Diabet Consortium TI Depressive Symptoms in Youth With Type 1 or Type 2 Diabetes: Results of the Pediatric Diabetes Consortium Screening Assessment of Depression in Diabetes Study SO DIABETES CARE LA English DT Article ID GLYCEMIC CONTROL; ADOLESCENTS; CHILDREN; CARE AB OBJECTIVETo evaluate the frequency of depressive symptoms and the diagnosis and management of depression in youth with type 1 diabetes (T1D) and type 2 diabetes (T2D) enrolled in the Pediatric Diabetes Consortium T1D and T2D registries.RESEARCH DESIGN AND METHODSThe Children's Depression Inventory (CDI) 2 Self-Report (Short) version was completed by 261 T1D and 339 T2D youth aged 10-17 years.RESULTSSymptoms of depression were identified in 13% of T1D and 22% of T2D (P = 0.007) participants; of these, only 4% of T1D and 9% of T2D youth were treated by a therapist within the prior 12 months. Depressive symptoms were associated with lower family income (P = 0.006) and obesity (P = 0.002) in T1D but not T2D youth.CONCLUSIONSDepressive symptoms are more frequent than diagnosed depression in youth with T1D or T2D. These results underscore the need for regular depression screening and appropriate referral for youth with diabetes. C1 [Silverstein, Janet] Univ Florida, Pediat Endocrinol, Gainesville, FL USA. [Cheng, Peiyao; Ruedy, Katrina J.; Kollman, Craig; Beck, Roy W.] Jaeb Ctr Hlth Res, Tampa, FL 33647 USA. [Klingensmith, Georgeanna J.] Univ Colorado, Barbara Davis Ctr Childhood Diabet, Dept Pediat, Aurora, CO USA. [Wood, Jamie R.] Childrens Hosp Los Angeles, Ctr Endocrinol Diabet & Metab, Los Angeles, CA 90027 USA. [Willi, Steven] Univ Penn, Perelman Sch Med, Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA. [Bacha, Fida] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Bacha, Fida; Redondo, Maria J.] Texas Childrens Hosp, Baylor Coll Med, Pediat Diabet & Endocrinol, Houston, TX 77030 USA. [Lee, Joyce] Univ Michigan, CS Mott Childrens Hosp, Dept Pediat Endocrinol, Ann Arbor, MI 48109 USA. [Cengiz, Eda; Tamborlane, William V.] Yale Univ, Pediat Endocrinol, New Haven, CT USA. RP Ruedy, KJ (reprint author), Jaeb Ctr Hlth Res, Tampa, FL 33647 USA. EM pdc@jaeb.org FU Novo Nordisk; Veroscience; AstraZeneca; Boehringer Ingelheim; Takeda; Janssen FX The PDC and its activities are supported by an unrestricted grant from Novo Nordisk. G.J.K. reports payment for educational consultation to Eli Lilly as the planner and participant in a continuing medical education course for the Eli Lilly Company and also is a paid consultant for Novo Nordisk. S.W. has grant funding from Novo Nordisk and Veroscience. W.V.T. is a paid consultant for Novo Nordisk, AstraZeneca, Boehringer Ingelheim, Takeda, and Janssen. No other potential conflicts of interest relevant to this article were reported. NR 15 TC 2 Z9 2 U1 3 U2 8 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0149-5992 EI 1935-5548 J9 DIABETES CARE JI Diabetes Care PD DEC PY 2015 VL 38 IS 12 BP 2341 EP 2343 DI 10.2337/dc15-0982 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CY0TK UT WOS:000366118800035 PM 26459274 ER PT J AU Taylor, AL Murphree, R Ingram, LA Garman, K Solomon, D Coffey, E Walker, D Rogers, M Marder, E Bottomley, M Woron, A Thomas, L Roberts, S Hardin, H Arjmandi, P Green, A Simmons, L Cornell, A Dunn, J AF Taylor, Amanda L. Murphree, Rendi Ingram, L. Amanda Garman, Katie Solomon, Deborah Coffey, Eric Walker, Deborah Rogers, Marsha Marder, Ellyn Bottomley, Marie Woron, Amy Thomas, Linda Roberts, Sheri Hardin, Henrietta Arjmandi, Parvin Green, Alice Simmons, Latoya Cornell, Allyson Dunn, John TI Multidrug-Resistant Salmonella Heidelberg Associated with Mechanically Separated Chicken at a Correctional Facility SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID OUTCOMES; SEROTYPE AB We describe multidrug-resistant (MDR) Salmonella Heidelberg infections associated with mechanically separated chicken (MSC) served at a county correctional facility. Twenty-three inmates met the case definition. All reported diarrhea, 19 (83%) reported fever, 16 (70%) reported vomiting, 4 (17%) had fever 103 degrees F, and 3 (13%) were hospitalized. A case-control study found no single food item significantly associated with illness. Salmonella Heidelberg with an indistinguishable pulsed-field gel electrophoresis pattern was isolated from nine stool specimens; two isolates displayed resistance to a total of five drug classes, including the third-generation cephalosporin, ceftriaxone. MDR Salmonella Heidelberg might have contributed to the severity of illness. Salmonella Heidelberg indistinguishable from the outbreak subtype was isolated from unopened MSC. The environmental health assessment identified cross-contamination through poor food-handling practices as a possible contributing factor. Proper hand-washing techniques and safe food-handling practices were reviewed with the kitchen supervisor. C1 [Taylor, Amanda L.; Solomon, Deborah; Coffey, Eric; Walker, Deborah; Rogers, Marsha; Cornell, Allyson] Tennessee Dept Hlth, Chattanooga, TN USA. [Murphree, Rendi; Marder, Ellyn; Simmons, Latoya] Ctr Dis Control & Prevent, Atlanta, GA USA. [Ingram, L. Amanda; Garman, Katie; Bottomley, Marie; Woron, Amy; Thomas, Linda; Roberts, Sheri; Hardin, Henrietta; Arjmandi, Parvin; Dunn, John] Tennessee Dept Hlth, Nashville, TN USA. [Green, Alice] USDA, Food Safety & Inspect Serv, Minneapolis, MN USA. RP Taylor, AL (reprint author), Georgia Dept Publ Hlth, 2 Peachtree St NW,16th Flr, Atlanta, GA 30303 USA. EM ataylo47@gmail.com FU TDH; CDC Epidemiology and Laboratory Capacity (ELC) FX The authors gratefully acknowledge the assistance and coordination between staff from TDH, USDA/FSIS, and CDC. We would also like to thank the MSC manufacturer for their decision to voluntarily recall the potentially contaminated product in an effort to prevent the spread of further illness. Funding for this outbreak investigation was provided by TDH. Salmonella surveillance and prevention was supported through a CDC Epidemiology and Laboratory Capacity (ELC) cooperative agreement. NR 13 TC 1 Z9 1 U1 3 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD DEC 1 PY 2015 VL 12 IS 12 BP 950 EP 952 DI 10.1089/fpd.2015.2008 PG 3 WC Food Science & Technology SC Food Science & Technology GA CY3SW UT WOS:000366331200004 PM 26540115 ER PT J AU Dargatz, DA Marshall, KL Fedorka-Cray, PJ Erdman, MM Kopral, CA AF Dargatz, David A. Marshall, Katherine L. Fedorka-Cray, Paula J. Erdman, Matthew M. Kopral, Christine A. TI Salmonella Prevalence and Antimicrobial Susceptibility from the National Animal Health Monitoring System Sheep 2011 Study SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID UNITED-STATES; ARIZONAE AB Salmonella is a major cause of foodborne illness and can cause clinical disease in animals. Understanding the on-farm ecology of Salmonella will be helpful in decreasing the risk of foodborne transmission. An objective of this study was to determine the prevalence of Salmonella among fecal samples collected on sheep operations in the United States. Another objective was to compare the use of composite fecal samples with fecal samples collected from individual sheep as a tool for screening sheep flocks for Salmonella. Sheep fecal samples (individual and composite) were collected on operations in 22 states. Salmonella isolates were characterized with regard to species, serotype, and antimicrobial susceptibility profile. Most operations (72.1%) had at least one positive sample and overall 26.9% of samples were positive. The percentage of positive samples varied by animal age class. Composite and individual samples gave similar results. The majority of the isolates (94%) were Salmonella enterica subspecies diarizonae serotype 61:-:1,5,7. Nearly all of the isolates (91.2%) tested for antimicrobial susceptibility were susceptible to all antimicrobials in the panel. The findings suggest that salmonellae typically associated with foodborne disease transmission are infrequently found on sheep operations in the United States. C1 [Dargatz, David A.; Marshall, Katherine L.; Kopral, Christine A.] USDA, Ctr Epidemiol & Anim Hlth, Anim & Plant Hlth Inspect Serv, Ft Collins, CO 80525 USA. [Fedorka-Cray, Paula J.] ARS, USDA, Bacterial Epidemiol & Antimicrobial Resistance Un, Athens, GA USA. [Erdman, Matthew M.] USDA, Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, Ames, IA USA. RP Dargatz, DA (reprint author), USDA, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80525 USA. EM david.a.dargatz@aphis.usda.gov NR 16 TC 1 Z9 1 U1 4 U2 8 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD DEC 1 PY 2015 VL 12 IS 12 BP 953 EP 957 DI 10.1089/fpd.2015.2016 PG 5 WC Food Science & Technology SC Food Science & Technology GA CY3SW UT WOS:000366331200005 PM 26540254 ER PT J AU Rebbeck, J Kloss, A Bowden, M Coon, C Hutchinson, TF Iverson, L Guess, G AF Rebbeck, Joanne Kloss, Aaron Bowden, Michael Coon, Cheryl Hutchinson, Todd F. Iverson, Louis Guess, Greg TI Aerial Detection of Seed-Bearing Female Ailanthus altissima: A Cost-Effective Method to Map an Invasive Tree in Forested Landscapes SO FOREST SCIENCE LA English DT Article DE woody invasive control; digital mapping technology; forest management ID VIRGINIA; GROWTH AB We present an aerial mapping method to efficiently and effectively identify seed clusters of the invasive tree, Ailanthus altissima (Mill.) Swingle across deciduous forest landscapes in the eastern United States. We found that the ideal time to conduct aerial digital surveys is early to middle winter, when Ailanthus seed clusters persist and there is no interfering leaf cover. Because of the color, quantity, and seedpod arrangement, female seed-bearing Ailanthus trees are very conspicuous from the air. With use of digital sketchmapping technology from helicopters, seed-bearing Ailanthus trees were mapped at a rate of 2,000-4,000 acres/hour (809-1,618 ha/hour). We estimated mapping costs at approximately $0.40/acre ($1.00/ha). We were able to relocate, within 100-200 ft, 88-95% of the aerially mapped seed-bearing Ailanthus trees using handheld consumer-grade global positioning systems (GPS) units. This provided enough accuracy to locate seed-bearing Ailanthus for single-stem injection herbicide treatments. To apply these methods to map seed-bearing Ailanthus, land management agencies that already use digital mapping technology (equipment and software) for surveys of insect and disease outbreaks will have minimal costs beyond helicopter time. C1 [Rebbeck, Joanne; Hutchinson, Todd F.; Iverson, Louis] USDA, Forest Serv, No Res Stn, Delaware, OH 43015 USA. [Kloss, Aaron; Guess, Greg] Ohio Dept Nat Resources, Div Forestry, Delaware, OH USA. [Bowden, Michael] Ohio Dept Nat Resources, Div Mineral Resources Management, Delaware, OH USA. [Coon, Cheryl] USDA, Forest Serv, Wayne Natl Forest, Delaware, OH USA. RP Rebbeck, J (reprint author), USDA, Forest Serv, No Res Stn, Delaware, OH 43015 USA. EM jrebbeck@fs.fed.us; aaron.kloss@dnr.state.oh.us; michael.bowden@dnr.state.oh.us; ccoon@fs.fed.us; thutchinson@fs.fed.us; liverson@fs.fed.us; Gregory.Guess@dnr.state.oh.us FU USDA Forest Service, Wayne National Forest; Joint Fire Science Program; USDA Forest Service, Northern Research Station FX We thank Joe Barber, Buster Keaton, Tom Shuman, Danzil Walker, and Courtney Streithorst, ODNR Divisions of Forestry and Wildlife, for assistance in the aerial mapping. We greatly appreciate the support of William Borovicka, Levi Miller, Timothy Fox, and Richard Craig for assistance in the field. We thank Tim Fox and Joan Jolliff for assistance with data entry. We thank Aurora Roemmich and Nicole Stump, USDA Forest Service, Wayne National Forest for their support and cooperation. We appreciate the support and cooperation from Robert Boyles, Dan Balser, and Chad Sanders of the Ohio Department of Natural Resources, Division of Forestry. We thank Robert Long and William Oldland for technical reviews and John Stanovick for biometrics review. Funding was provided by the Joint Fire Science Program and the USDA Forest Service, Northern Research Station. NR 23 TC 0 Z9 0 U1 2 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD DEC PY 2015 VL 61 IS 6 BP 1068 EP 1078 DI 10.5849/forsci.14-223 PG 11 WC Forestry SC Forestry GA CY1DJ UT WOS:000366146600009 ER PT J AU Klotz, JL AF Klotz, J. L. TI BILL E. KUNKLE INTERDISCIPLINARY BEEF SYMPOSIUM: Physiologic effects of ergot alkaloids: What happens when excretion does not equal consumption? SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; ergot alkaloids; physiology ID INFECTED TALL FESCUE; LATERAL SAPHENOUS VEINS; PERFORMANCE LIQUID-CHROMATOGRAPHY; SEROTONIN RECEPTORS; IN-VITRO; FLUOROMETRIC DETECTION; ERGOPEPTINE ALKALOIDS; DOPAMINE-RECEPTORS; MASS-SPECTROMETRY; ENDOPHYTE-FREE AB Increased persistence of tall fescue (Lolium arundinaceum) infested with an endophytic fungus, Epichloe coenophiala (formerly Neotyphodium coenophialum), in forage-based agriculture has led to increased effort in understanding the negative effects caused by consumption of ergot alkaloids by animals consuming this forage. Ergot alkaloids have been shown to have an extremely short plasma half-life, but this does not necessarily equate to total clearance. Studies that measured consumption and excretion of alkaloids have demonstrated that in the case of ergovaline, less is excreted than is consumed. The fate of ergot alkaloids that leave circulation but are not excreted is not well understood. Consequently, these "alkaloid balance studies" have led to speculation that ergovaline might bioaccumulate in the animal. Unfortunately, few data indisputably support this outcome. Progress has been slowed by the fact that the fungus produces a multitude of different ergot alkaloids that can bind to a variety of different receptors. Binding studies have shown that ergot alkaloids have unusually slow receptor dissociation rates that have been described as irreversible and contribute to a persistent signaling effect. In vitro analyses have revealed a potential for accumulation of ergot alkaloids through repetitive exposures to low concentrations creating a "depot" of alkaloids available to interact with receptors. The specific high binding affinity of ergot alkaloids combined with the potential turnover of alkaloids bound nonspecifically could extend residual effects of these compounds. Interestingly, cattle exposed to ergot alkaloids in vivo have a consistently lower vascular response to agonists that target receptors known to bind ergot alkaloids. If these same receptors are blocked with an antagonist, contractile response to ergopeptine alkaloids is also reduced significantly (>60% reduction). This observation that alkaloid exposure interrupts normal function of a receptor can persist 5 to 6 wk after animals have been removed from an ergot alkaloid source (and prolactin levels have long since returned to normal). Thus, clearance of ergot alkaloids from cattle grazing pasture with ergot alkaloid-producing endophytes may occur in a similar gradual manner. Studies that improve the understanding of how cattle process ergot alkaloids will help answer the question of whether ergot alkaloids bioaccumulate. C1 [Klotz, J. L.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. RP Klotz, JL (reprint author), Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. EM james.klotz@ars.usda.gov NR 63 TC 1 Z9 1 U1 2 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD DEC PY 2015 VL 93 IS 12 BP 5512 EP 5521 DI 10.2527/jas.2015-9261 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY3RI UT WOS:000366327000005 PM 26641161 ER PT J AU Rubio, YLB Duarte, JLG Bates, RO Ernst, CW Nonneman, D Rohrer, GA King, DA Shackelford, SD Wheeler, TL Cantet, RJC Steibel, JP AF Bernal Rubio, Y. L. Gualdron Duarte, J. L. Bates, R. O. Ernst, C. W. Nonneman, D. Rohrer, G. A. King, D. A. Shackelford, S. D. Wheeler, T. L. Cantet, R. J. C. Steibel, J. P. TI Implementing meta-analysis from genome-wide association studies for pork quality traits SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE candidate genes; genome-wide association; meat quality; meta-analysis; pigs ID SINGLE NUCLEOTIDE POLYMORPHISM; UNFOLDED PROTEIN RESPONSE; BRATZLER SHEAR FORCE; PIG SKELETAL-MUSCLE; MEAT QUALITY; ENDOPLASMIC-RETICULUM; BEEF-CATTLE; MU-CALPAIN; IMPUTATION ACCURACY; GENOTYPE IMPUTATION AB Pork quality plays an important role in the meat processing industry. Thus, different methodologies have been implemented to elucidate the genetic architecture of traits affecting meat quality. One of the most common and widely used approaches is to perform genome-wide association (GWA) studies. However, a limitation of many GWA in animal breeding is the limited power due to small sample sizes in animal populations. One alternative is to implement a meta-analysis of GWA (MA-GWA) combining results from independent association studies. The objective of this study was to identify significant genomic regions associated with meat quality traits by performing MA-GWA for 8 different traits in 3 independent pig populations. Results from MA-GWA were used to search for genes possibly associated with the set of evaluated traits. Data from 3 pig data sets (U.S. Meat Animal Research Center, commercial, and Michigan State University Pig Resource Population) were used. A MA was implemented by combining z-scores derived for each SNP in every population and then weighting them using the inverse of estimated variance of SNP effects. A search for annotated genes retrieved genes previously reported as candidates for shear force (calpain-1 catalytic subunit [CAPN1] and calpastatin [CAST]), as well as for ultimate pH, purge loss, and cook loss (protein kinase, AMP-activated,. 3 noncatalytic subunit [PRKAG3]). In addition, novel candidate genes were identified for intramuscular fat and cook loss (acyl-CoA synthetase family member 3 mitochondrial [ACSF3]) and for the objective measure of muscle redness, CIEa* (glycogen synthase 1, muscle [GYS1] and ferritin, light polypeptide [FTL]). Thus, implementation of MA-GWA allowed integration of results for economically relevant traits and identified novel genes to be tested as candidates for meat quality traits in pig populations. C1 [Bernal Rubio, Y. L.; Gualdron Duarte, J. L.; Cantet, R. J. C.] UBA, Fac Agron, Dept Prod Anim, Buenos Aires, DF, Argentina. [Bernal Rubio, Y. L.; Bates, R. O.; Ernst, C. W.; Steibel, J. P.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Nonneman, D.; Rohrer, G. A.; King, D. A.; Shackelford, S. D.; Wheeler, T. L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Cantet, R. J. C.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Steibel, J. P.] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA. RP Steibel, JP (reprint author), Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. EM steibelj@msu.edu OI Cantet, Rodolfo/0000-0001-6282-146X FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2010-65205-20342]; National Pork Board [11-042]; US Pig Genome Coordination funds; Michigan Animal Initiative Coalition Grant FX This work was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010-65205-20342 from the USDA National Institute of Food and Agriculture, and by funding from the National Pork Board Grant no. 11-042. Partial support was also provided by US Pig Genome Coordination funds and a Michigan Animal Initiative Coalition Grant. Computer resources were provided by the Michigan State University High Performance Computing Center (HPCC). USDA is an equal opportunity provider and employer. NR 78 TC 3 Z9 3 U1 1 U2 9 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD DEC PY 2015 VL 93 IS 12 BP 5607 EP 5617 DI 10.2527/jas.2015-9502 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY3RI UT WOS:000366327000014 ER PT J AU Adeola, O Azain, MJ Carter, SD Crenshaw, TD Estienne, MJ Kerr, BJ Lindemann, MD Maxwell, CV Miller, PS Shannon, MC van Heugten, E AF Adeola, O. Azain, M. J. Carter, S. D. Crenshaw, T. D. Estienne, M. J. Kerr, B. J. Lindemann, M. D. Maxwell, C. V. Miller, P. S. Shannon, M. C. van Heugten, E. CA North Cent Coordinating Comm Swine TI A cooperative study on the standardized total-tract digestible phosphorus requirement of twenty-kilogram pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE phosphorus; pigs; requirement; standardized total-tract digestible ID GROWING-FINISHING PIGS; RELATIVE BIOAVAILABILITY; DIETARY CALCIUM; MINERAL BALANCE; YOUNG-PIGS; PERFORMANCE; PHYTASE; VALUES; LEVEL; SWINE AB A cooperative study comprising growth performance, bone mineralization, and nutrient balance experiments was conducted at 11 stations to determine the standardized total-tract digestible (STTD) P requirement of 20-kg pigs using broken-line regression analysis. Monocalcium phosphate and limestone were added to a corn-soybean meal-based diet at the expense of cornstarch to establish 6 concentrations of STTD P from 1.54 to 5.15 g/kg in increments of 0.62 g/kg at a constant Ca:total P of 1.52:1.0. Diets were fed to 936 pigs (average initial BW of 19 kg) in 240 pens for 20 replicate pens of barrows and 20 replicate pens of gilts per diet. As STTD P increased from 1.54 to 5.15 g/kg of the diet for d 0 to 14, 14 to 28, and 0 to 28, the ADG, ADFI, and G:F increased (P < 0.01). Barrows gained and ate more (P < 0.05) than gilts during d 14 to 28 and 0 to 28. There was no interaction between sex and STTD P concentration for any of the growth performance response criteria. There were both linear and quadratic increases (P < 0.05) in mineral density and content of ash, Ca, and P in the femur expressed as a percentage of dry, fat-free metacarpal as dietary STTD P increased. Furthermore, the maximum load of the femur and mineral density and content and maximum load as well as the Ca and P expressed as a percentage of metacarpal ash linearly increased (P < 0.01) with increasing dietary concentrations of STTD P. There were both linear and quadratic increases (P < 0.01) in apparent digestibility and retention of P with increasing concentrations of STTD P in the diets. Digestibility and retention of Ca linearly (P < 0.01) increased with increasing dietary concentrations of STTD P. Breakpoints determined from nonlinear broken-line regression analyses revealed estimates of 4.20 +/- 0.102, 3.20 +/- 0.036, or 3.87 +/- 0.090 g/kg for ADG during d 0 to 14, 14 to 28, or 0 to 28, respectively. Corresponding estimates using G: F as the response criterion were 4.34 +/- 0.146, 3.38 +/- 0.139, or 4.08 +/- 0.195 g/kg. When mineralization of the femur was used as criteria of response, estimates of STTD P requirement were 4.28, 4.28, or 4.34, g/kg for mineral density, mineral content, or maximum load, respectively. Using mineralization of the metacarpal as criteria of response, estimates of STTD P requirement ranged from 3.5 to 5.0 g/kg depending on the metacarpal response criteria. The study provided empirical estimates of STTD P requirements of 20- to 40-kg pigs. C1 [Adeola, O.] Purdue Univ, W Lafayette, IN 47907 USA. [Azain, M. J.] Univ Georgia, Athens, GA 30602 USA. [Carter, S. D.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Crenshaw, T. D.] Univ Wisconsin, Madison, WI 53706 USA. [Estienne, M. J.] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA. [Kerr, B. J.] USDA ARS, Ames, IA 50011 USA. [Lindemann, M. D.] Univ Kentucky, Lexington, KY 40546 USA. [Maxwell, C. V.] Univ Arkansas, Fayetteville, AR 72701 USA. [Miller, P. S.] Univ Nebraska, Lincoln, NE 68583 USA. [Shannon, M. C.] Univ Missouri, Columbia, MO 65201 USA. [van Heugten, E.] N Carolina State Univ, Raleigh, NC 27695 USA. RP Adeola, O (reprint author), Purdue Univ, W Lafayette, IN 47907 USA. EM ladeola@purdue.edu NR 29 TC 2 Z9 2 U1 7 U2 13 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD DEC PY 2015 VL 93 IS 12 BP 5743 EP 5753 DI 10.2527/jas.2015-9509 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY3RI UT WOS:000366327000028 PM 26641184 ER PT J AU Cribbs, JT Bernhard, BC Young, TR Jennings, MA Sanchez, NCB Carroll, JA Callaway, TR Schmidt, TB Johnson, BJ Rathmann, RJ AF Cribbs, J. T. Bernhard, B. C. Young, T. R. Jennings, M. A. Sanchez, N. C. Burdick Carroll, J. A. Callaway, T. R. Schmidt, T. B. Johnson, B. J. Rathmann, R. J. TI Dehydrated citrus pulp alters feedlot performance of crossbred heifers during the receiving period and modulates serum metabolite concentrations before and after an endotoxin challenge SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE acute phase response; dehydrated citrus pulp; feedlot cattle; receiving cattle ID RUMINAL MICROORGANISMS; ENERGY-SOURCE; SUPPLEMENTATION; PRODUCTS; CATTLE; STEERS; DIETS; DIGESTIBILITY; POPULATIONS; MOLASSES AB English x Continental heifers (n = 180) were sourced in 2 loads (219.3 +/- 16.0 and 221.4 +/- 16.4 kg, respectively) from commercial auction barns to study the effects of feeding dehydrated citrus pulp (DCP) on feedlot performance of newly received heifers. A completely randomized block design was used with BW nested within arrival load and blocked by BW into 3 dietary treatments (36 pens, 5 heifers/pen, 12 blocks, 3 pens/block, and 12 pens/treatment). Treatment diets contained 1) 0% DCP (control diet [CON]), 2) 10% DCP, or 3) 20% DCP on a DM basis. Diets containing DCP were exchanged with steam-flaked corn on a 1: 1 basis. Cattle were fed a 63, 73, and 83% concentrate diet from d 0 to 28, d 28 to 42, and d 42 to 56, respectively. Over the 56-d trial period, as the amount of dietary DCP increased, DMI decreased (P = 0.01), ADG decreased (P < 0.01), and G:F decreased (P = 0.02). From d 0 to 28, there was no difference in the observed minus the predicted NEg of the diet (P = 0.73); from d 28 to 42, there was a linear increase in NEg favoring DCP treatments (P < 0.01); and from d 42 to 56, there was a linear decrease in NEg against the DCP treatments (P < 0.01). At the conclusion of the trial, a subset of heifers (n = 22; 307.89 +/- 3.32 kg on d 63) were used to evaluate blood metabolite concentrations before and after a lipopolysaccharide (LPS) challenge. On d 63, heifers were fitted with jugular catheters and moved into individual stalls. On d 64, heifers were intravenously challenged with LPS (0.5 mu g/kg BW), and blood samples were collected every 0.5 h from -2 to 8 h and at 24 h relative to the LPS challenge (0 h). Serum glucose, serum urea nitrogen (SUN), and NEFA concentrations were determined. Cattle lost less weight at both 24 and 72 h after the LPS challenge with increasing DCP percentage (P < 0.01). Glucose (P = 0.12) and NEFA (P = 0.13) concentrations did not differ before the LPS challenge; however, there was a treatment effect for SUN, with elevated concentrations of SUN in CON cattle (P < 0.01). After the LPS challenge, DCP-fed cattle had reduced glucose, elevated NEFA, and reduced SUN concentrations (P = 0.01). Results indicate that dietary DCP modulated metabolite concentrations in heifers following an endotoxin challenge and affected feedlot performance when incorporated in receiving diets in replacement of corn. Future studies will need to address strategies to increase DMI or explore levels of DCP less than 10% in the diet of newly received heifer calves. C1 [Cribbs, J. T.; Bernhard, B. C.; Young, T. R.; Jennings, M. A.; Johnson, B. J.; Rathmann, R. J.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Sanchez, N. C. Burdick; Carroll, J. A.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Callaway, T. R.] USDA ARS, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Schmidt, T. B.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. RP Rathmann, RJ (reprint author), Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. EM ryan.rathmann@ttu.edu NR 30 TC 0 Z9 0 U1 4 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD DEC PY 2015 VL 93 IS 12 BP 5791 EP 5800 DI 10.2527/jas.2015-9571 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY3RI UT WOS:000366327000033 PM 26641189 ER PT J AU Boyd, BM Shackelford, SD Hales, KE Brown-Brandl, TM Bremer, ML Spangler, ML Wheeler, TL King, DA Erickson, GE AF Boyd, B. M. Shackelford, S. D. Hales, K. E. Brown-Brandl, T. M. Bremer, M. L. Spangler, M. L. Wheeler, T. L. King, D. A. Erickson, G. E. TI Effects of shade and feeding zilpaterol hydrochloride to finishing steers on performance, carcass quality, heat stress, mobility, and body temperature SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE body temperature; mobility; respiration rate; shade; zilpaterol hydrochloride ID FEEDLOT CATTLE PERFORMANCE; DURATION; BEHAVIOR; AGONISTS; TRAITS AB Steers (n = 480; 22% with black hides and 78% with red hides) were used to study the effects of shade and feeding zilpaterol hydrochloride (ZH) on performance, carcass quality, heat stress, mobility, and body temperature (BT). A randomized block design with a 2 x 2 factorial treatment arrangement was used with 4 replicates per treatment. Factors included housing type (open or shaded pens) and the feeding of ZH (0 or 8.33 mg/kg DM) the last 21 d on feed with a 3-d withdrawal. Cattle were blocked by BW into a heavy or light block and randomly assigned to pen within each block. Rumen boluses to record BT were inserted before ZH feeding. Respiration rate and panting scores were recorded daily during the ZH feeding period. Mobility scores were collected at various time points from before ZH feeding through harvest. Interactions between ZH and housing type were not significant (P > 0.26) for animal performance, carcass characteristics, and respiration or panting score. No differences (P > 0.44) were observed for DMI, ADG, or G:F on a live basis due to ZH; however, cattle fed in open pens tended (P = 0.08) to have a greater ADG than cattle in shaded pens. Cattle fed ZH had 14 kg heavier carcasses with larger LM area (P < 0.01) than control cattle. Respiration rates for cattle fed ZH were greater (P = 0.05) with no differences (P = 0.88) due to housing. Time affected (P < 0.01) mobility scores, with observations on the morning of harvest at the abattoir being the worst for all groups of cattle. An interaction (P < 0.01) was observed between ZH and housing type for BT. Cattle fed ZH, in both shaded and open pens, had lower (P < 0.05) average, maximum, and area under the curve BT than control cattle fed in the same housing type. However, the observed reduction in BT due to ZH was greater for cattle fed ZH in open pens than for cattle fed ZH in shaded pens. From these results, we conclude that ZH improved HCW with little impact on heat stress or mobility, suggesting that animal welfare was not affected by feeding ZH for 21 d at the end of the feeding period. C1 [Boyd, B. M.; Bremer, M. L.; Spangler, M. L.; Erickson, G. E.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Shackelford, S. D.; Hales, K. E.; Brown-Brandl, T. M.; Wheeler, T. L.; King, D. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Hales, KE (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov; gerickson4@unl.edu OI Spangler, Matthew/0000-0001-5184-501X FU Hatch Act; Nebraska Beef Council FX A contribution of the University of Nebraska Agricultural Research Division, supported in part by funds provided through the Hatch Act. Research partially supported by a grant from the Nebraska Beef Council. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. The authors have no conflict of interest. The efforts of C. Engle, B. Johnson, L. McPhillips, C. Felber, D. Janssen, A. Menke, C. C. Row, J. Buntyn, R. Oglesbee, Z. Carlson, and the entire feedlot crew at U.S. MARC are greatly appreciated. NR 27 TC 3 Z9 3 U1 0 U2 9 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD DEC PY 2015 VL 93 IS 12 BP 5801 EP 5811 DI 10.2527/jas.2015-9613 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CY3RI UT WOS:000366327000034 PM 26641190 ER PT J AU Calle, A Porto-Fett, ACS Shoyer, BA Luchansky, JB Thippareddi, H AF Calle, Alexandra Porto-Fett, Anna C. S. Shoyer, Bradley A. Luchansky, John B. Thippareddi, Harshavardhan TI Microbiological Safety of Commercial Prime Rib Preparation Methods: Thermal Inactivation of Salmonella in Mechanically Tenderized Rib Eye SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157H7; TOXIN-PRODUCING O157/H7; FLAME GAS GRILL; UNITED-STATES; BEEF STEAKS; COOKING; PREVALENCE; O157-H7; FATE; CONTAMINANT AB Boneless beef rib eye roasts were surface inoculated on the fat side with ca. 5.7 log CFU/g of a five-strain cocktail of Salmonella for subsequent searing, cooking, and warm holding using preparation methods practiced by restaurants surveyed in a medium-size Midwestern city. A portion of the inoculated roasts was then passed once through a mechanical blade tenderizer. For both intact and nonintact roasts, searing for 15 min at 260 degrees C resulted in reductions in Salmonella populations of ca. 0.3 to 1.3 log CFU/g. For intact (nontenderized) rib eye roasts, cooking to internal temperatures of 37.8 or 48.9 degrees C resulted in additional reductions of ca. 3.4 log CFU/g. For tenderized (nonintact) rib eye roasts, cooking to internal temperatures of 37.8 or 48.9 degrees C resulted in additional reductions of ca. 3.1 or.3.4 log CFU/g, respectively. Pathogen populations remained relatively unchanged for intact roasts cooked to 37.8 or 48.9 degrees C and for nonintact roasts cooked to 48.9 degrees C when held at 60.0 degrees C for up to 8 h. In contrast, pathogen populations increased ca. 2.0 log CFU/g in nonintact rib eye cooked to 37.8 degrees C when held at 60.0 degrees C for 8 h. Thus, cooking at low temperatures and extended holding at relatively low temperatures as evaluated herein may pose a food safety risk to consumers in terms of inadequate lethality and/or subsequent outgrowth of Salmonella, especially if nonintact rib eye is used in the preparation of prime rib, if on occasion appreciable populations of Salmonella are present in or on the meat, and/or if the meat is not cooked adequately throughout. C1 [Calle, Alexandra; Thippareddi, Harshavardhan] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. [Porto-Fett, Anna C. S.; Shoyer, Bradley A.; Luchansky, John B.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Thippareddi, H (reprint author), Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. EM harsha.thippareddi@uga.edu NR 45 TC 1 Z9 1 U1 4 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD DEC PY 2015 VL 78 IS 12 BP 2126 EP 2135 DI 10.4315/0362-028X.JFP-15-154 PG 10 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CY0AB UT WOS:000366066900003 PM 26613906 ER PT J AU Guo, M Buchanan, RL Dubey, JP Hill, DE Lambertini, E Ying, YQ Gamble, HR Jones, JL Pradhan, AK AF Guo, Miao Buchanan, Robert L. Dubey, Jitender P. Hill, Dolores E. Lambertini, Elisabetta Ying, Yuqing Gamble, H. Ray Jones, Jeffrey L. Pradhan, Abani K. TI Qualitative Assessment for Toxoplasma gondii Exposure Risk Associated with Meat Products in the United States SO JOURNAL OF FOOD PROTECTION LA English DT Article ID REAL-TIME-PCR; TISSUE CYSTS; DIFFERENT STRAINS; PORK; INFECTIVITY; PREVALENCE; ANIMALS; SAFETY; PIG; QUANTIFICATION AB Toxoplasma gondii is a global protozoan parasite capable of infecting most warm-blooded animals. Although healthy adult humans generally have no symptoms, severe illness does occur in certain groups, including congenitally infected fetuses and newborns, immunocompromised individuals including transplant patients. Epidemiological studies have demonstrated that consumption of raw or undercooked meat products is one of the major sources of infection with T. gondii. The goal of this study was to develop a framework to qualitatively estimate the exposure risk to T. gondii from various meat products consumed in the United States. Risk estimates of various meats were analyzed by a farm-to-retail qualitative assessment that included evaluation of farm, abattoir, storage and transportation, meat processing, packaging, and retail modules. It was found that exposure risks associated with meats from free-range chickens, nonconfinement-raised pigs, goats, and lamb are higher than those from confinement-raised pigs, cattle, and caged chickens. For fresh meat products, risk at the retail level was similar to that at the farm level unless meats had been frozen or moisture enhanced. Our results showed that meat processing, such as salting, freezing, commercial hot air drying, long fermentation times, hot smoking, and cooking, are able to reduce T. gondii levels in meat products. whereas nitrite and/or nitrate, spice, low pH, and cold storage have no effect on the viability of T. gondii tissue cysts. Raw-fermented sausage, cured raw meat, meat that is not hot-air dried, and fresh processed meat were associated with higher exposure risks compared with cooked meat and frozen meat. This study provides a reference for meat management control programs to determine critical control points and serves as the foundation for future quantitative risk assessments. C1 [Guo, Miao; Buchanan, Robert L.; Lambertini, Elisabetta; Ying, Yuqing; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Buchanan, Robert L.; Lambertini, Elisabetta; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Dubey, Jitender P.; Hill, Dolores E.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Gamble, H. Ray] Natl Acad Sci, Washington, DC 20001 USA. [Jones, Jeffrey L.] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM akp@umd.edu FU U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative [2012-67005-19611] FX This work was supported through a grant from the U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (award 2012-67005-19611). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the NIFA, the Centers for Disease Control and Prevention, or the USDA. NR 80 TC 1 Z9 1 U1 5 U2 22 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD DEC PY 2015 VL 78 IS 12 BP 2207 EP 2219 DI 10.4315/0362-028X.JFP-15-270 PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CY0AB UT WOS:000366066900013 PM 26613916 ER PT J AU Ferreira, RB Beard, KH Choi, RT Pitt, WC AF Ferreira, Rodrigo B. Beard, Karen H. Choi, Ryan T. Pitt, William C. TI Diet of the Nonnative Greenhouse Frog (Eleutherodactylus planirostris) in Maui, Hawaii SO JOURNAL OF HERPETOLOGY LA English DT Article ID PACIFIC ISLAND; INVERTEBRATE COMMUNITIES; ANURANS; COQUI; VEGETATION; SELECTION; ECOLOGY; LITTER AB The Greenhouse Frog (Eleutherodactylus planirostris) is one of the most widespread frog species in the world. Because of its high population densities, widespread distribution, and consumption of native invertebrates in some invaded sites, understanding its impacts in Hawaii is important. We analyzed stomach contents of 397 frogs from 10 study sites in Maui. Results suggest Greenhouse Frogs are active, ant-specialist predators in the leaf litter. Ants (Formicidae) were the dominant prey found in stomachs in both number and volume. Furthermore, only ants were consumed in a higher proportion than they were sampled in the environment. Because ants dominated their diets, and because all ants are nonnative to Hawaii, this means Greenhouse Frogs consumed primarily nonnative invertebrates (>80%) in the areas sampled. Although results suggest that most native taxa are not at risk from Greenhouse Frog predation, the only locations where we could currently find Greenhouse Frogs were in human-dominated lowlands, which have a lower proportion of native species. Greenhouse Frogs may consume more native species if they invade more native-dominated habitat. Alternatively, nonnative ants are known to impact negatively many native invertebrates in Hawaii, and their possible reduction through Greenhouse Frog predation could affect other species positively. Our research highlights the need to understand better the effects of Greenhouse Frog predation on both native and nonnative invertebrates in Hawaii. C1 [Ferreira, Rodrigo B.; Beard, Karen H.; Choi, Ryan T.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Ferreira, Rodrigo B.; Beard, Karen H.; Choi, Ryan T.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Pitt, William C.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Hawaii Field Stn, Hilo, HI 96721 USA. RP Beard, KH (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM karen.beard@usu.edu RI Beard, Karen/B-7177-2011 FU Jack Berryman Institute, USDA Wildlife Services NWRC Hawaii Field Station, and USU Ecology Center; Utah Agricultural Experiment Station, Utah State University; Institute Animal Care and Use Committee approval [1402] FX We thank the Jack Berryman Institute, USDA Wildlife Services NWRC Hawaii Field Station, and USU Ecology Center for funding. This research was supported by the Utah Agricultural Experiment Station, Utah State University, and approved as journal paper number 8687. We thank S. Rodrigues for assistance during fieldwork, and J. Gonzalez for sorting invertebrates from environment samples. Research was conducted under Institute Animal Care and Use Committee approval (Protocol 1402) and the following State of Hawaii permits: Injurious Wildlife Export and DLNR/DSP Scientific Research, DLNR/DOFAW Access to Land and Native Invertebrate. NR 44 TC 1 Z9 1 U1 1 U2 12 PU SOC STUDY AMPHIBIANS REPTILES PI ST LOUIS PA C/O ROBERT D ALDRIDGE, ST LOUIS UNIV, DEPT BIOLOGY, 3507 LACLEDE, ST LOUIS, MO 63103 USA SN 0022-1511 EI 1937-2418 J9 J HERPETOL JI J. Herpetol. PD DEC PY 2015 VL 49 IS 4 BP 586 EP 593 DI 10.1670/14-103 PG 8 WC Zoology SC Zoology GA CY1PY UT WOS:000366181100011 ER PT J AU Tagtow, A Nguyen, J Johnson-Bailey, D Schap, TE AF Tagtow, Angle Nguyen, Jimmy Johnson-Bailey, Donna Schap, TusaRebecca E. TI Food Waste Reduction Efforts at the USDA SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article C1 [Tagtow, Angle; Johnson-Bailey, Donna; Schap, TusaRebecca E.] USDA Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. [Nguyen, Jimmy] USDA Food & Nutr Serv, Alexandria, VA USA. RP Tagtow, A (reprint author), USDA Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 1 TC 0 Z9 0 U1 4 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2015 VL 115 IS 12 BP 1914 EP 1918 DI 10.1016/j.jand.2015.10.008 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX9DF UT WOS:000366004400002 PM 26613647 ER PT J AU Zizza, CA Sebastian, RS Enns, CW Isik, Z Goldman, JD Moshfegh, AJ AF Zizza, Claire A. Sebastian, Rhonda S. Enns, Cecilia Wilkinson Isik, Zeynep Goldman, Joseph D. Moshfegh, Alanna J. TI The Contribution of Beverages to Intakes of Energy and MyPlate Components by Current, Former, and Never Smokers in the United States SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Dietary assessment; Beverages; Smoking; United States ID ALCOHOL-CONSUMPTION; CIGARETTE-SMOKING; HEALTH BEHAVIORS; NATIONAL-HEALTH; DIETARY-INTAKE; US ADULTS; RISK; LIFE; FOOD; PREVALENCE AB Background Although beverage intake patterns have been shown to differ by smoking status, it is unknown whether the contributions of beverages to intakes of energy and MyPlate components also differ. Objective The purpose of this study was to compare beverage intakes and contributions of energy and MyPlate components by source (food alone, beverages alone, and food and beverages together) in diets of adult current, former, and never smokers. Design and participants Dietary data from 4,823 men and 4,672 women aged >= 20 years who participated in What We Eat in America, National Health and Nutrition Examination Survey 2005-2008, were analyzed. Main outcome measures Beverage intake and the contributions to energy and MyPlate components by beverages. Statistical analysis Regression analyses identified differences in intake among groups. Results Current smokers consumed more total beverages, coffee, and sugar-sweetened beverages than never and former smokers (P<0.001). Male current smokers drank more alcoholic beverages than never and former smokers, whereas female current and former smokers both consumed more alcoholic beverages than never smokers. Current smokers obtained more energy from beverages than their nonsmoking counterparts, although total energy intake did not differ. Intakes of added sugars, alcohol, and empty calories were higher for current than never smokers, and differences were accounted for by current smokers' beverage choices. Conclusions This study adds to the body of research on smoking and dietary behavior by showing that not only do smokers consume a higher volume of beverages, but they also have a higher intake of energy provided by beverages, mainly empty calories from added sugars and alcohol. Our findings highlight the importance of assessing beverages' contribution to the total diet. Recognizing the common co-occurrence of smoking and specific beverage choices can help target health promotion and disease prevention efforts for this subpopulation. C1 [Zizza, Claire A.; Isik, Zeynep] Auburn Univ, Dept Nutr Dietet & Hospitality Management, Auburn, AL 36849 USA. [Sebastian, Rhonda S.; Enns, Cecilia Wilkinson; Goldman, Joseph D.; Moshfegh, Alanna J.] ARS, Food Surveys Res Grp, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Sebastian, RS (reprint author), USDA Food Surveys Res Grp, BARC West,Bldg 005,Room 102,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Rhonda.Sebastian@ars.usda.gov FU Alabama Agricultural Experiment Station FX C. A. Zizza is supported in part by the Alabama Agricultural Experiment Station. NR 34 TC 0 Z9 0 U1 2 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2015 VL 115 IS 12 BP 1939 EP 1949 DI 10.1016/j.jand.2015.07.015 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX9DF UT WOS:000366004400005 PM 26362079 ER PT J AU Thompson, FE Kirkpatrick, SI Subar, AF Reedy, J Schap, TE Wilson, MM Krebs-Smith, SM AF Thompson, Frances E. Kirkpatrick, Sharon I. Subar, Amy F. Reedy, Jill Schap, TusaRebecca E. Wilson, Magdalena M. Krebs-Smith, Susan M. TI The National Cancer Institute's Dietary Assessment Primer: A Resource for Diet Research SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Dietary assessment; Diet instrument; 24-Hour dietary recall; Food record; Food frequency questionnaire ID FOOD FREQUENCY QUESTIONNAIRE; DOUBLY LABELED WATER; RECOVERY BIOMARKERS; MEASUREMENT ERROR; ASSESSMENT INSTRUMENTS; ENERGY; RECORDS; VALIDATION; POTASSIUM; VALIDITY AB This monograph describes the National Cancer Institute's Dietary Assessment Primer, a web resource developed to help researchers choose the best available dietary assessment approach to achieve their research objective. All self-report instruments have error, but understanding the nature of that error can lead to better assessment, analysis, and interpretation of results. The Primer includes profiles of the major self-report dietary assessment instruments, including guidance on the best uses of each instrument; discussion of validation and measurement error generally and with respect to each instrument; guidance for choosing a dietary assessment approach for different research questions; and additional resources, such as a glossary, references, and overviews of specific/important issues in the field. This monograph also describes some future research needs in the field of dietary assessment. C1 [Thompson, Frances E.; Subar, Amy F.; Reedy, Jill; Schap, TusaRebecca E.; Wilson, Magdalena M.; Krebs-Smith, Susan M.] NCI, Risk Factor Assessment Branch, Epidemiol & Genom Res Program, Div Canc Control & Populat Sci,NIH, Bethesda, MD 20892 USA. [Kirkpatrick, Sharon I.] Univ Waterloo, Sch Publ Hlth & Hlth Syst, Waterloo, ON N2L 3G1, Canada. [Schap, TusaRebecca E.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. RP Thompson, FE (reprint author), NCI, Risk Factor Assessment Branch, Epidemiol & Genom Res Program, Div Canc Control & Populat Sci,NIH, 9609 Med Ctr Dr,MSC 9762, Bethesda, MD 20892 USA. EM thompsof@mail.nih.gov OI Kirkpatrick, Sharon/0000-0001-9896-5975 FU Intramural NIH HHS [Z99 CA999999] NR 42 TC 7 Z9 7 U1 2 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD DEC PY 2015 VL 115 IS 12 BP 1986 EP 1995 DI 10.1016/j.jand.2015.08.016 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX9DF UT WOS:000366004400010 PM 26422452 ER PT J AU Ming, R VanBuren, R Wai, CM Tang, HB Schatz, MC Bowers, JE Lyons, E Wang, ML Chen, J Biggers, E Zhang, JS Huang, LX Zhang, LM Miao, WJ Zhang, J Ye, ZY Miao, CY Lin, ZC Wang, H Zhou, HY Yim, WC Priest, HD Zheng, CF Woodhouse, M Edger, PP Guyot, R Guo, HB Guo, H Zheng, GY Singh, R Sharma, A Min, XJ Zheng, Y Lee, H Gurtowski, J Sedlazeck, FJ Harkess, A McKain, MR Liao, ZY Fang, JP Liu, J Zhang, XD Zhang, Q Hu, WC Qin, Y Wang, K Chen, LY Shirley, N Lin, YR Liu, LY Hernandez, AG Wright, CL Bulone, V Tuskan, GA Heath, K Zee, F Moore, PH Sunkar, R Leebens-Mack, JH Mockler, T Bennetzen, JL Freeling, M Sankoff, D Paterson, AH Zhu, XG Yang, XH Smith, JAC Cushman, JC Paull, RE Yu, QY AF Ming, Ray VanBuren, Robert Wai, Ching Man Tang, Haibao Schatz, Michael C. Bowers, John E. Lyons, Eric Wang, Ming-Li Chen, Jung Biggers, Eric Zhang, Jisen Huang, Lixian Zhang, Lingmao Miao, Wenjing Zhang, Jian Ye, Zhangyao Miao, Chenyong Lin, Zhicong Wang, Hao Zhou, Hongye Yim, Won C. Priest, Henry D. Zheng, Chunfang Woodhouse, Margaret Edger, Patrick P. Guyot, Romain Guo, Hao-Bo Guo, Hong Zheng, Guangyong Singh, Ratnesh Sharma, Anupma Min, Xiangjia Zheng, Yun Lee, Hayan Gurtowski, James Sedlazeck, Fritz J. Harkess, Alex McKain, Michael R. Liao, Zhenyang Fang, Jingping Liu, Juan Zhang, Xiaodan Zhang, Qing Hu, Weichang Qin, Yuan Wang, Kai Chen, Li-Yu Shirley, Neil Lin, Yann-Rong Liu, Li-Yu Hernandez, Alvaro G. Wright, Chris L. Bulone, Vincent Tuskan, Gerald A. Heath, Katy Zee, Francis Moore, Paul H. Sunkar, Ramanjulu Leebens-Mack, James H. Mockler, Todd Bennetzen, Jeffrey L. Freeling, Michael Sankoff, David Paterson, Andrew H. Zhu, Xinguang Yang, Xiaohan Smith, J. Andrew C. Cushman, John C. Paull, Robert E. Yu, Qingyi TI The pineapple genome and the evolution of CAM photosynthesis SO NATURE GENETICS LA English DT Article ID CRASSULACEAN ACID METABOLISM; LTR RETROTRANSPOSONS; EXPRESSION ANALYSIS; ANANAS-COMOSUS; GENE; SEQUENCE; PLANTS; ARABIDOPSIS; IDENTIFICATION; DIVERGENCE AB Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 and MD2 and a wild pineapple relative, Ananas bracteatus accession CB5. The pineapple genome has one fewer ancient whole-genome duplication event than sequenced grass genomes and a conserved karyotype with seven chromosomes from before the. duplication event. The pineapple lineage has transitioned from C-3 photosynthesis to CAM, with CAM-related genes exhibiting a diel expression pattern in photosynthetic tissues. CAM pathway genes were enriched with cis-regulatory elements associated with the regulation of circadian clock genes, providing the first cis-regulatory link between CAM and circadian clock regulation. Pineapple CAM photosynthesis evolved by the reconfiguration of pathways in C-3 plants, through the regulatory neofunctionalization of preexisting genes and not through the acquisition of neofunctionalized genes via whole-genome or tandem gene duplication. C1 [Ming, Ray; VanBuren, Robert; Wai, Ching Man; Tang, Haibao; Zhang, Jisen; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Zhang, Jian; Ye, Zhangyao; Miao, Chenyong; Lin, Zhicong; Liao, Zhenyang; Fang, Jingping; Liu, Juan; Zhang, Xiaodan; Zhang, Qing; Hu, Weichang; Qin, Yuan; Wang, Kai; Chen, Li-Yu] Fujian Agr & Forestry Univ, Fujian Agr & Forestry Univ & Univ Illinois Urbana, Fuzhou, Peoples R China. [Ming, Ray; VanBuren, Robert; Wai, Ching Man; Tang, Haibao; Zhang, Jisen; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Zhang, Jian; Ye, Zhangyao; Miao, Chenyong; Lin, Zhicong; Liao, Zhenyang; Fang, Jingping; Liu, Juan; Zhang, Xiaodan; Zhang, Qing; Hu, Weichang; Qin, Yuan; Wang, Kai; Chen, Li-Yu] Fujian Agr & Forestry Univ, Fujian Taiwan Joint Ctr Ecol Control Crop Pests, Fuzhou, Peoples R China. [Ming, Ray; VanBuren, Robert; Wai, Ching Man; Heath, Katy] Univ Illinois, Dept Plant Biol, Urbana, IL USA. [VanBuren, Robert; Priest, Henry D.; McKain, Michael R.; Mockler, Todd] Donald Danforth Plant Sci Ctr, St Louis, MO USA. [Tang, Haibao; Lyons, Eric] Univ Arizona, iPlant Collaborat, Tucson, AZ USA. [Schatz, Michael C.; Biggers, Eric; Lee, Hayan; Gurtowski, James; Sedlazeck, Fritz J.] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Bowers, John E.; Wang, Hao; Zhou, Hongye; Harkess, Alex; Leebens-Mack, James H.; Bennetzen, Jeffrey L.] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA. [Wang, Ming-Li; Moore, Paul H.] Hawaii Agr Res Ctr, Kunia, HI USA. [Chen, Jung; Paull, Robert E.] Univ Hawaii, Dept Trop Plant & Soil Sci, Honolulu, HI 96822 USA. [Yim, Won C.; Cushman, John C.] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA. [Zheng, Chunfang; Sankoff, David] Univ Ottawa, Dept Math & Stat, Ottawa, ON, Canada. [Woodhouse, Margaret; Edger, Patrick P.; Freeling, Michael] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Guyot, Romain] IRD, Divers Adaptat & Dev Plantes, Montpellier, France. [Guo, Hao-Bo; Guo, Hong] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN USA. [Zheng, Guangyong; Zhu, Xinguang] Chinese Acad Sci, Shanghai Inst Biol Sci, Max Planck Gesell Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China. [Singh, Ratnesh; Sharma, Anupma; Yu, Qingyi] Texas A&M Univ Syst, Dept Plant Pathol & Microbiol, Texas A&M AgriLife Res, Dallas, TX USA. [Min, Xiangjia] Youngstown State Univ, Dept Biol Sci, Youngstown, OH 44555 USA. [Zheng, Yun] Kunming Univ Sci & Technol, Fac Life Sci & Technol, Kunming, Peoples R China. [Shirley, Neil; Bulone, Vincent] Univ Adelaide, Sch Agr Food & Wine, Australian Res Council ARC Ctr Excellence Plant C, Adelaide, SA, Australia. [Lin, Yann-Rong; Liu, Li-Yu] Natl Taiwan Univ, Dept Agron, Taipei, Taiwan. [Hernandez, Alvaro G.; Wright, Chris L.] Univ Illinois, WM Keck Ctr, Urbana, IL USA. [Tuskan, Gerald A.; Yang, Xiaohan] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA. [Zee, Francis] USDA ARS, Pacific Basin Agr Res Ctr, Hilo, HI USA. [Sunkar, Ramanjulu] Oklahoma State Univ, Dept Biochem & Mol Biol, Noble Res Ctr, Stillwater, OK 74078 USA. [Paterson, Andrew H.] Univ Georgia, Plant Genome Mapping Lab, Athens, GA 30602 USA. [Smith, J. Andrew C.] Univ Oxford, Dept Plant Sci, Oxford, England. RP Ming, R (reprint author), Fujian Agr & Forestry Univ, Fujian Agr & Forestry Univ & Univ Illinois Urbana, Fuzhou, Peoples R China. EM rming@life.uiuc.edu; qyu@ag.tamu.edu RI Yim, Won Cheol/K-9100-2016; Bowers, John/B-9245-2009; Yang, Xiaohan/A-6975-2011; Mockler, Todd/L-2609-2013; Tuskan, Gerald/A-6225-2011; Zheng, Yun/I-7011-2012; Bulone, Vincent/D-7469-2013; guyot, romain/M-1118-2015; Guo, Hao-Bo/B-7486-2009; OI Yim, Won Cheol/0000-0002-7489-0435; Yang, Xiaohan/0000-0001-5207-4210; Mockler, Todd/0000-0002-0462-5775; Tuskan, Gerald/0000-0003-0106-1289; Zheng, Yun/0000-0003-4292-9806; Lin, Yann-rong/0000-0001-6833-8276; Liu, Li-yu/0000-0001-6997-8101; guyot, romain/0000-0002-7016-7485; Guo, Hao-Bo/0000-0003-1321-1758; Min, Xiangjia/0000-0001-7978-0596; Sedlazeck, Fritz/0000-0001-6040-2691 FU Fujian Agriculture and Forestry University; USDA T-START grant through the University of Hawaii; University of Illinois at Urbana-Champaign; Fujian provincial government; US National Science Foundation (NSF) Plant Genome Program [0922545]; NSF [DBI-1401572, IOS-1444567]; US National Institutes of Health [R01-HG006677]; US NSF [DBI-1350041, DBI-1265383]; US Department of Energy, Office of Science, Genomic Science Program [DE-SC0008834] FX We thank R. Kai and C. Mayo Riley for maintaining the pineapple plants and the collection of leaf tissues; M. Conway at Dole Plantation for assistance in time-course leaf sample collection; G. Sanewski for providing the MD2 pedigree; and M. Cushman for providing clarifying comments on the manuscript. This project is supported by funding from the Fujian Agriculture and Forestry University to R. M.; a USDA T-START grant through the University of Hawaii to Q.Y., R.M., P.H.M. and R.E.P.; and funding from the University of Illinois at Urbana-Champaign to R.M. H.T. is supported by the 100 Talent Plan award from the Fujian provincial government. Analyses of the pineapple genome are supported by the following funding sources: US National Science Foundation (NSF) Plant Genome Program grant 0922545 to R.M., P.H.M. and Q.Y. and NSF grant DBI-1401572 to R.V.; NSF grant IOS-1444567 to J.H.L.-M.; and US National Institutes of Health award R01-HG006677 and US NSF awards DBI-1350041 and DBI-1265383 to M.C.S. W.C.Y., H.-B.G., H.G., G.A.T., X.Y. and J.C.C. acknowledge support from the US Department of Energy, Office of Science, Genomic Science Program, under award DE-SC0008834. NR 66 TC 33 Z9 35 U1 16 U2 89 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD DEC PY 2015 VL 47 IS 12 BP 1435 EP + DI 10.1038/ng.3435 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA CX6LT UT WOS:000365813200015 PM 26523774 ER PT J AU Sosso, D Luo, DP Li, QB Sasse, J Yang, JL Gendrot, G Suzuki, M Koch, KE McCarty, DR Chourey, PS Rogowsky, PM Ross-Ibarra, J Yang, B Frommer, WB AF Sosso, Davide Luo, Dangping Li, Qin-Bao Sasse, Joelle Yang, Jinliang Gendrot, Ghislaine Suzuki, Masaharu Koch, Karen E. McCarty, Donald R. Chourey, Prem S. Rogowsky, Peter M. Ross-Ibarra, Jeffrey Yang, Bing Frommer, Wolf B. TI Seed filling in domesticated maize and rice depends on SWEET-mediated hexose transport SO NATURE GENETICS LA English DT Article ID CELL-WALL INVERTASE; ZEA-MAYS-L; GENE-EXPRESSION; ARABIDOPSIS; ENDOSPERM; GENOME; CARBON; DIFFERENTIATION; TRANSCRIPTION; METABOLISM AB Carbohydrate import into seeds directly determines seed size and must have been increased through domestication. However, evidence of the domestication of sugar translocation and the identities of seed-filling transporters have been elusive. Maize ZmSWEET4c, as opposed to its sucrose-transporting homologs, mediates transepithelial hexose transport across the basal endosperm transfer layer (BETL), the entry point of nutrients into the seed, and shows signatures indicative of selection during domestication. Mutants of both maize ZmSWEET4c and its rice ortholog OsSWEET4 are defective in seed filling, indicating that a lack of hexose transport at the BETL impairs further transfer of sugars imported from the maternal phloem. In both maize and rice, SWEET4 was likely recruited during domestication to enhance sugar import into the endosperm. C1 [Sosso, Davide; Sasse, Joelle; Frommer, Wolf B.] Carnegie Sci, Dept Plant Biol, Stanford, CA 94305 USA. [Luo, Dangping; Yang, Bing] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA. [Li, Qin-Bao; Chourey, Prem S.] USDA ARS, Gainesville, FL USA. [Yang, Jinliang] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Gendrot, Ghislaine; Rogowsky, Peter M.] Univ Lyon 1, Ecole Normale Super Lyon, Unite Reprod & Dev Plantes, F-69365 Lyon, France. [Suzuki, Masaharu; Koch, Karen E.; McCarty, Donald R.; Chourey, Prem S.] Univ Florida, Dept Agron, Plant Mol & Cellular Biol, Gainesville, FL 32611 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Ctr Populat Biol, Dept Plant Sci, Davis, CA 95616 USA. [Ross-Ibarra, Jeffrey] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. RP Sosso, D (reprint author), Carnegie Sci, Dept Plant Biol, Stanford, CA 94305 USA. EM dsosso@carnegiescience.edu OI Rogowsky, Peter/0000-0003-4822-3783 FU Office of Basic Energy Sciences of the US Department of Energy [DE-FG02-04ER15542]; National Science Foundation [IOS-1258018, IOS-1116561, IOS-1025976, IOS-1238014]; USDA-NIFA [2010-04228] FX We are grateful to D. Ehrhardt and H. Cartwright for confocal microscopy. For the rice experiments, we are grateful to T. Li for constructing the TALEN vector targeting OsSWEET4, B. Liu for rice transgenics, C. Ji for the isolation and transfection of rice protoplasts in the laboratory of B. Y. and X. Li for help with domestication analysis of OsSWEET4. We thank M. Greenfield, A. Grimault and K.M. Wong for plant care, Y. Gong for the yeast complementation assay, M. Evans for providing teosinte plant material and C. Stefan for renaming ZmSWEET4 with "c" for her initial. Work performed on maize in the laboratory of W.B.F. was made possible by support from the Office of Basic Energy Sciences of the US Department of Energy under grant DE-FG02-04ER15542, and work on rice was supported by the National Science Foundation under grant IOS-1258018 (B.Y. and W.B.F.); the other laboratories were supported by the National Science Foundation (IOS-1116561 to D.R.M. and K.E.K., IOS-1025976 to K.E.K. and IOS-1238014 to J.R.-I.), as well as USDA-NIFA 2010-04228 (K.E.K., D.R.M. and M.S.). NR 61 TC 13 Z9 14 U1 13 U2 49 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD DEC PY 2015 VL 47 IS 12 BP 1489 EP + DI 10.1038/ng.3422 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA CX6LT UT WOS:000365813200023 PM 26523777 ER PT J AU Muller, AC Muller, DL AF Muller, Andrew C. Muller, Diana Lynn TI Forecasting future estuarine hypoxia using a wavelet based neural network model SO OCEAN MODELLING LA English DT Article DE Hypoxia; Estuarine dynamics; Neural network models; El Nino; Wavelet coherence; Chesapeake Bay ID WIND-INDUCED DESTRATIFICATION; BAYESIAN HIERARCHICAL MODEL; PARTIALLY-MIXED ESTUARY; NEUSE RIVER ESTUARY; CHESAPEAKE-BAY; WATER-QUALITY; AQUATIC VEGETATION; NORTH-CAROLINA; DEAD ZONES; ECOSYSTEM AB Ecosystem based modeling and predictions of hypoxia in estuaries and their adjacent coastal areas have become increasingly of interest to researchers and coastal zone managers. Although progress has been made in modeling oxygen dynamics and short-term predictions, there is still a lack of long-term forecasts that incorporate multiple inputs including climatological effects such as El Nino-Southern Oscillation (ENSO) events. In this study, we first develop a hypoxic volume index (HV1) using 26-years of hypoxic volume (<62.5 mu m gl(-1)) measurements from the main-stem of the Chesapeake Bay. Then a cross-wavelet analysis is used to identify and weight input parameters in order to build a neural network model of future hypoxic volume. The time-forward dynamic model uses cross-bay winds along with the Oceanic Nino Index (ONI), and Susquehanna River flow indexes to predict a hypoxic volume index over the next several years. Wavelet analysis indicates an anti-phase relationship between southwesterly winds and hypoxic volume index, and an 18-month phase lag between Susquehanna River index and hypoxic volume index. The neural network model results yield R-values of 0.99, and 0.91 for training, and validation and an R-2 of 0.68 for predictions illustrating the usefulness and promise of these types of models for long-term predictions of hypoxic volume. Model results could be used as a climatologically based hypoxic volume baseline for comparing actual hypoxic volume response to nutrient load reductions. Published by Elsevier Ltd. C1 [Muller, Andrew C.] USDA, Dept Oceanog, Annapolis, MD 21402 USA. RP Muller, AC (reprint author), USDA, Dept Oceanog, Annapolis, MD 21402 USA. EM amuller@usna.edu; captdianalynn@gmail.com FU Naval Academy Research Council; United States Naval Academy Oceanography Department; Paul and Maxine Frohring Foundation FX We would like to thank the Naval Academy Research Council, the United States Naval Academy Oceanography Department, and the Paul and Maxine Frohring Foundation for their support on this project. We would also like to thank the two anonymous reviewers for helping to greatly improve the manuscript. NR 94 TC 0 Z9 0 U1 3 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1463-5003 EI 1463-5011 J9 OCEAN MODEL JI Ocean Model. PD DEC PY 2015 VL 96 BP 314 EP 323 DI 10.1016/j.ocemod.2015.11.003 PN 2 PG 10 WC Meteorology & Atmospheric Sciences; Oceanography SC Meteorology & Atmospheric Sciences; Oceanography GA CX9XA UT WOS:000366058600011 ER PT J AU Bernardo, A Wang, S St Amand, P Bai, GH AF Bernardo, Amy Wang, Shan St Amand, Paul Bai, Guihua TI Using Next Generation Sequencing for Multiplexed Trait-Linked Markers in Wheat SO PLOS ONE LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISM; PREHARVEST SPROUTING RESISTANCE; HARD WINTER-WHEAT; HEXAPLOID WHEAT; MOLECULAR MARKERS; GENES; IDENTIFICATION; VALIDATION; CLONING; ARRAY AB With the advent of next generation sequencing (NGS) technologies, single nucleotide polymorphisms (SNPs) have become the major type of marker for genotyping in many crops. However, the availability of SNP markers for important traits of bread wheat (Triticum aestivum L.) that can be effectively used in marker-assisted selection (MAS) is still limited and SNP assays for MAS are usually uniplex. A shift from uniplex to multiplex assays will allow the simultaneous analysis of multiple markers and increase MAS efficiency. We designed 33 locus-specific markers from SNP or indel-based marker sequences that linked to 20 different quantitative trait loci (QTL) or genes of agronomic importance in wheat and analyzed the amplicon sequences using an Ion Torrent Proton Sequencer and a custom allele detection pipeline to determine the genotypes of 24 selected germplasm accessions. Among the 33 markers, 27 were successfully multiplexed and 23 had 100% SNP call rates. Results from analysis of "kompetitive allele-specific PCR" (KASP) and sequence tagged site (STS) markers developed from the same loci fully verified the genotype calls of 23 markers. The NGS-based multiplexed assay developed in this study is suitable for rapid and high-throughput screening of SNPs and some indel-based markers in wheat. C1 [Bernardo, Amy] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Wang, Shan; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [St Amand, Paul; Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS USA. RP Bai, GH (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. EM guihua.bai@ars.usda.gov FU National Research Initiative Competitive Grants CAP from the USDA National Institute of Food and Agriculture [2011-68002-30029]; US Wheat and Barley Scab Initiative FX This project is partly funded by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and US Wheat and Barley Scab Initiative. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 1 Z9 1 U1 8 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 1 PY 2015 VL 10 IS 12 AR e0143890 DI 10.1371/journal.pone.0143890 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7OL UT WOS:000365891600069 PM 26625271 ER PT J AU Hashemian, M Ryu, D Crow, WT Kustas, WP AF Hashemian, Minoo Ryu, Dongryeol Crow, Wade T. Kustas, William P. TI Improving root-zone soil moisture estimations using dynamic root growth and crop phenology SO ADVANCES IN WATER RESOURCES LA English DT Article DE Soil moisture; Land surface modelling; Root distribution; Transpiration; Soil vegetation atmosphere transfer (SVAT) ID WATER-UPTAKE MODEL; LAND-SURFACE MODEL; VEGETATION INDEXES; BALANCE MODEL; ASSIMILATION; WHEAT; PERFORMANCE; SIMULATION AB Water Energy Balance (WEB) Soil Vegetation Atmosphere Transfer (SVAT) modelling can be used to estimate soil moisture by forcing the model with observed data such as precipitation and solar radiation. Recently, an innovative approach that assimilates remotely sensed thermal infrared (TIR) observations into WEB-SVAT to improve the results has been proposed. However, the efficacy of the model observation integration relies on the model's realistic representation of soil water processes. Here, we explore methods to fin prove the soil water processes of a simple WEB-SVAT model by adopting and incorporating an exponential root water uptake model with water stress compensation and establishing a more appropriate soil-biophysical linkage between root-zone moisture content, above-ground states and biophysical indices. The existing WEB-SVAT model is extended to a new Multi-layer WEB-SVAT with Dynamic Root distribution (MWSDR) that has five soil layers. Impacts of plant root depth variations, growth stages and phenological cycle of the vegetation on transpiration are considered in developing stages. Hydrometeorological and biogeophysical measurements collected from two experimental sites, one in Dookie, Victoria, Australia and the other in Ponca, Oklahoma, USA, are used to validate the new model. Results demonstrate that MWSDR provides improved soil moisture, transpiration and evaporation predictions which, in turn, can provide an improved physical basis for assimilating remotely sensed data into the model. Results also show the importance of having an adequate representation of vegetation-related transpiration process for an appropriate simulation of water transfer in a complicated system of soil, plants and atmosphere. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hashemian, Minoo; Ryu, Dongryeol] Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic 3010, Australia. [Crow, Wade T.; Kustas, William P.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Hashemian, M (reprint author), Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic 3010, Australia. EM minooh@student.unimelb.edu.au OI Ryu, Dongryeol/0000-0002-5335-6209 NR 42 TC 2 Z9 2 U1 2 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD DEC PY 2015 VL 86 BP 170 EP 183 DI 10.1016/j.advwatres.2015.10.001 PN A PG 14 WC Water Resources SC Water Resources GA CX3TT UT WOS:000365623000013 ER PT J AU Gebauer, SK Destaillats, F Dionisi, F Krauss, RM Baer, DJ AF Gebauer, Sarah K. Destaillats, Frederic Dionisi, Fabiola Krauss, Ronald M. Baer, David J. TI Vaccenic acid and trans fatty acid isomers from partially hydrogenated oil both adversely affect LDL cholesterol: a double-blind, randomized controlled trial SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE cardiovascular disease risk; industrial trans fatty acids; partially hydrogenated vegetable oil; ruminant trans fatty acids; vaccenic acid ID CONJUGATED LINOLEIC-ACID; CORONARY-HEART-DISEASE; DENSITY LIPOPROTEIN CHOLESTEROL; APOLIPOPROTEIN-A-I; C-REACTIVE PROTEIN; CARDIOVASCULAR-DISEASE; RISK-FACTORS; RUMINANT TRANS; BLOOD-LIPIDS; ENZYMATIC DETERMINATION AB Background: Adverse effects of industrially produced trans fatty acids (iTFAs) on the risk of coronary artery disease are well documented in the scientific literature; however, effects of naturally occurring trans fatty acids (TFAs) from ruminant animals (rTFA), such as vaccenic acid (VA) and cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA), are less clear. Although animal and cell studies suggest that VA and c9, t11-CLA may be hypocholesterolemic and antiatherogenic, epidemiologic data comparing rTFAs and iTFAs are inconsistent, and human intervention studies have been limited, underpowered, and not well controlled. Objective: We determined the effects of VA, c9, t11-CLA, and iTFA, in the context of highly controlled diets (24 d each), on lipoprotein risk factors compared with a control diet. Results: We conducted a double-blind, randomized, crossover feeding trial in 106 healthy adults [mean +/- SD age: 47 +/- 10.8 y; body mass index (in kg/m(2)): 28.5 +/- 4.0; low-density lipoprotein (LDL) cholesterol: 3.24 +/- 0.63 mmol/L]. Diets were designed to have stearic acid replaced with the following TFA isomers (percentage of energy): 0.1% mixed isomers of TFA (control), similar to 3% VA, similar to 3% iTFA, or 1% c9, t11-CLA. Total dietary fat (34% of energy) and other macronutrients were matched. Total cholesterol (TC), LDL cholesterol, triacylglycerol, lipoprotein(a), and apolipoprotein B were higher after VA than after iTFA; high-density lipoprotein (HDL) cholesterol and apolipoprotein AI also were higher after VA. Compared with control, VA and iTFA both increased TC, LDL cholesterol, ratio of TC to HDL cholesterol, and apolipoprotein B (2-6% change; P < 0.05); VA also increased HDL cholesterol, apolipoprotein AI, apolipoprotein B, and lipoprotein(a) (2-6% change; P < 0.05), whereas iTFA did not. c9, t11-CLA lowered triacylglycerol (P <= 0.01) and had no effect on other lipoprotein risk factors. Conclusions: With respect to risk of cardiovascular disease, these results are consistent with current nutrition labeling guidelines, with the requirement of VA, but not c9, t11-CLA, to be listed under TFA on the Nutrition Facts Panel. C1 [Gebauer, Sarah K.; Baer, David J.] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Destaillats, Frederic; Dionisi, Fabiola] Nestle Res Ctr, CH-1000 Lausanne, Switzerland. [Krauss, Ronald M.] Childrens Hosp Oakland, Res Inst, Oakland, CA 94609 USA. RP Baer, DJ (reprint author), ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. EM david.baer@ars.usda.gov FU USDA; Dairy Management Inc.; Nestle; Dairy Australia FX Supported by USDA, Dairy Management Inc., Nestle, and Dairy Australia. The funding organizations had no role in the conduct of the study; collection, management, analysis, and interpretation of the data; or decision to submit the manuscript for publication. All four funding organizations reviewed and approved the manuscript. NR 48 TC 8 Z9 8 U1 7 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD DEC PY 2015 VL 102 IS 6 BP 1339 EP 1346 DI 10.3945/ajcn.115.116129 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX5BY UT WOS:000365717300007 PM 26561632 ER PT J AU Moran, NE Cichon, MJ Riedl, KM Grainger, EM Schwartz, SJ Novotny, JA Erdman, JW Clinton, SK AF Moran, Nancy E. Cichon, Morgan J. Riedl, Kenneth M. Grainger, Elizabeth M. Schwartz, Steven J. Novotny, Janet A. Erdman, John W., Jr. Clinton, Steven K. TI Compartmental and noncompartmental modeling of C-13-lycopene absorption, isomerization, and distribution kinetics in healthy adults SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE compartmental modeling; isomers; kinetics; lycopene; tracers ID BETA-CAROTENE; IN-VITRO; LYCOPENE ISOMERIZATION; PROSTATE-CANCER; GEOMETRICAL-ISOMERS; TOMATO PRODUCTS; TRANS LYCOPENE; HUMANS; BIOAVAILABILITY; METABOLISM AB Background: Lycopene, which is a red carotenoid in tomatoes, has been hypothesized to mediate disease-preventive effects associated with tomato consumption. Lycopene is consumed primarily as the all-trans geometric isomer in foods, whereas human plasma and tissues show greater proportions of cis isomers. Objective: With the use of compartmental modeling and stable isotope technology, we determined whether endogenous all-trans-to-cis-lycopene isomerization or isomeric-bioavailability differences underlie the greater proportion of lycopene cis isomers in human tissues than in tomato foods. Design: Healthy men (n = 4) and women (n = 4) consumed C-13-lycopene (10.2 mg; 82% all-trans and 18% cis), and plasma was collected over 28 d. Unlabeled and C-13-labeled total lycopene and lycopene-isomer plasma concentrations, which were measured with the use of high-performance liquid chromatography-mass spectrometry, were fit to a 7-compartment model. Results: Subjects absorbed a mean 6 SEM of 23% +/- 6% of the lycopene. The proportion of plasma cis-C-13-lycopene isomers increased over time, and all-trans had a shorter half-life than that of cis isomers (5.3 +/- 0.3 and 8.8 +/- 0.6 d, respectively; P < 0.001) and an earlier time to reach maximal plasma concentration than that of cis isomers (28 +/- 7 and 48 +/- 9 h, respectively). A compartmental model that allowed for interindividual differences in cis- and all-trans-lycopene bioavailability and endogenous trans-to-cis-lycopene isomerization was predictive of plasma C-13 and unlabeled cis- and all-trans-lycopene concentrations. Although the bioavailability of cis (24.5% +/- 6%) and all-trans (23.2% +/- 8%) isomers did not differ, endogenous isomerization (0.97 +/- 0.25 mu mol/d in the fast-turnover tissue lycopene pool) drove tissue and plasma isomeric profiles. Conclusion: C-13-Lycopene combined with physiologic compartmental modeling provides a strategy for following complex in vivo metabolic processes in humans and reveals that postabsorptive trans-to-cis-lycopene isomerization, and not the differential bioavailability of isomers, drives tissue and plasma enrichment of cis-lycopene. C1 [Moran, Nancy E.; Riedl, Kenneth M.; Grainger, Elizabeth M.; Schwartz, Steven J.; Clinton, Steven K.] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA. [Cichon, Morgan J.; Riedl, Kenneth M.; Schwartz, Steven J.] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA. [Clinton, Steven K.] Ohio State Univ, Internal Med Div Med Oncol, Columbus, OH 43210 USA. [Clinton, Steven K.] Ohio State Univ, James Canc Hosp, Columbus, OH 43210 USA. [Novotny, Janet A.] USDA, Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Erdman, John W., Jr.] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Erdman, John W., Jr.] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA. RP Clinton, SK (reprint author), Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA. EM steven.clinton@osumc.edu RI Riedl, Ken/G-8621-2014 OI Riedl, Ken/0000-0002-9020-3471 FU NIH National Center for Complementary and Alternative Medicine [5R21AT005166]; James Cancer Hospital's Bionutrition and Chemoprevention Fund [310684]; Ohio State University Center for Clinical and Translational Science (from the National Center for Advancing Translational Sciences) [UL1TR001070]; Ohio State University Comprehensive Cancer Center and its Nutrient and Phytochemical Analytic Shared Resource [P30CA016058]; Pelotonia postdoctoral fellowship FX Supported by a grant from the NIH National Center for Complementary and Alternative Medicine (5R21AT005166), The James Cancer Hospital's Bionutrition and Chemoprevention Fund (310684), The Ohio State University Center for Clinical and Translational Science (grant UL1TR001070 from the National Center for Advancing Translational Sciences), The Ohio State University Comprehensive Cancer Center and its Nutrient and Phytochemical Analytic Shared Resource (grant P30CA016058), and a Pelotonia postdoctoral fellowship (to NEM). Some carotenoid standards were donated by BASF and DSM. NR 55 TC 5 Z9 5 U1 1 U2 6 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD DEC PY 2015 VL 102 IS 6 BP 1436 EP 1449 DI 10.3945/ajcn.114.103143 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX5BY UT WOS:000365717300018 PM 26561629 ER PT J AU Allen, LH Hampel, D Shahab-Ferdows, S York, ER Adair, LS Flax, VL Tegha, G Chasela, CS Kamwendo, D Jamieson, DJ Bentley, ME AF Allen, Lindsay H. Hampel, Daniela Shahab-Ferdows, Setareh York, Emily R. Adair, Linda S. Flax, Valerie L. Tegha, Gerald Chasela, Charles S. Kamwendo, Debbie Jamieson, Denise J. Bentley, Margaret E. TI Antiretroviral therapy provided to HIV-infected Malawian women in a randomized trial diminishes the positive effects of lipid-based nutrient supplements on breast-milk B vitamins SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE antiretrovirals; B vitamins; breast milk; human milk; ultraperformance liquid chromatography tandem mass spectrometry ID TO-CHILD TRANSMISSION; HUMAN-IMMUNODEFICIENCY-VIRUS; FEEDING INFANTS; PREVENTION; BOTSWANA; PLUS; BAN; PROPHYLAXIS; PREGNANCY; REGIMENS AB Background: Little information is available on B vitamin concentrations in human milk or on how they are affected by maternal B vitamin deficiencies, antiretroviral therapy, or maternal supplementation. Objective: The objective was to evaluate the effects of antiretroviral therapy and/or lipid-based nutrient supplements (LNSs) on B vitamin concentrations in breast milk from HIV-infected women in Malawi. Design: Breast milk was collected from 537 women recruited within the Breastfeeding, Antiretrovirals, and Nutrition study at 2 or 6 wk and 24 wk postpartum. Women were assigned to receive antiretrovirals and LNSs, antiretrovirals only, LNSs only, or a control. Antiretrovirals and LNSs were given to the mothers from weeks 0 to 28. The antiretrovirals were zidovudine/lamivudine and nelfinavir or lopinavir/ritonavir. LNSs provided 93-118% of the Recommended Dietary Allowances of thiamin, riboflavin, niacin, pyridoxine, and vitamin B-12. Infants were exclusively breastfed. Results: LNSs increased milk concentrations of all vitamins except thiamin, whereas antiretrovirals lowered concentrations of nicotinamide, pyridoxal, and vitamin B-12. Although antiretrovirals alone had no significant effect on riboflavin concentrations, they negatively affected the LNS-induced increase in this vitamin. Thiamin was not influenced by the study interventions. Concentrations of all B vitamins were much lower than usually accepted values. Conclusions: All B vitamins were low in milk, and all but thiamin were increased by maternal supplementation with LNSs. Antiretrovirals alone decreased concentrations of some B vitamins in milk. When LNS was given in addition to antiretrovirals, the negative effect of antiretrovirals offset the positive effect of LNSs for all vitamins except thiamin. C1 [Allen, Lindsay H.; Hampel, Daniela; Shahab-Ferdows, Setareh; York, Emily R.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Allen, Lindsay H.; Hampel, Daniela; York, Emily R.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Adair, Linda S.; Flax, Valerie L.; Bentley, Margaret E.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Nutr, Chapel Hill, NC USA. [Jamieson, Denise J.] CDC, Atlanta, GA 30333 USA. [Tegha, Gerald; Chasela, Charles S.; Kamwendo, Debbie] UNC Project, Lilongwe, Malawi. [Chasela, Charles S.] Univ Witwatersrand, Sch Publ Hlth, Div Epidemiol & Biostat, Johannesburg, South Africa. RP Allen, LH (reprint author), ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. EM lindsay.allen@ars.usda.gov FU Prevention Research Centers Special Interest Project of the CDC [SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, SIP 22-09 U48-DP001944-01]; Bill & Melinda Gates Foundation [OPP53107, OPP1061055]; National Institute of Allergy and Infectious Diseases; University of North Carolina Center for AIDS Research [P30-AI50410]; Carolina Population Center [R24 HD050924]; NIH Fogarty AIDS International Training and Research Program [DHHS/NIH/FIC 2-D43 Tw01039-06, R24 Tw00798]; NIH Fogarty AIDS International Training and Research Program (American Recovery and Reinvestment Act); intramural USDA-ARS Project [5306-51000-003-00D]; Elizabeth Glaser Pediatric AIDS Foundation; United Nations Children's Fund; World Food Program; Malawi Ministry of Health and Population; Johnson Johnson; US Agency for International Development FX The Breastfeeding, Antiretrovirals, and Nutrition Study was supported by grants from the Prevention Research Centers Special Interest Project of the CDC (SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, and SIP 22-09 U48-DP001944-01); the Bill & Melinda Gates Foundation (OPP53107 and OPP1061055); the National Institute of Allergy and Infectious Diseases; the University of North Carolina Center for AIDS Research (P30-AI50410); the Carolina Population Center (R24 HD050924); the NIH Fogarty AIDS International Training and Research Program (DHHS/NIH/FIC 2-D43 Tw01039-06 and R24 Tw00798; the American Recovery and Reinvestment Act); and the intramural USDA-ARS Project (5306-51000-003-00D). The antiretrovirals used in the BAN study were donated by Abbott Laboratories, GlaxoSmithKline, Boehringer Ingelheim, Roche Pharmaceuticals, and Bristol-Myers Squibb. The Call to Action PMTCT program was supported by the Elizabeth Glaser Pediatric AIDS Foundation, the United Nations Children's Fund, the World Food Program, the Malawi Ministry of Health and Population, Johnson & Johnson, and the US Agency for International Development. NR 38 TC 4 Z9 4 U1 0 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD DEC PY 2015 VL 102 IS 6 BP 1468 EP 1474 DI 10.3945/ajcn.114.105106 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CX5BY UT WOS:000365717300021 PM 26537941 ER PT J AU Reid, KF Price, LL Harvey, WF Driban, JB Hau, C Fielding, RA Wang, CC AF Reid, Kieran F. Price, Lori Lyn Harvey, William F. Driban, Jeffrey B. Hau, Cynthia Fielding, Roger A. Wang, Chenchen TI Muscle Power Is an Independent Determinant of Pain and Quality of Life in Knee Osteoarthritis SO ARTHRITIS & RHEUMATOLOGY LA English DT Article ID HEALTH SURVEY SF-36; QUADRICEPS STRENGTH; OLDER-ADULTS; WOMEN; PERFORMANCE; PROGRESSION; DISABILITY; INCIDENT; VELOCITY; THERAPY AB Objective. This study examined the relationships between leg muscle strength, power, and perceived disease severity in subjects with knee osteoarthritis (OA) in order to determine whether dynamic leg extensor muscle power would be associated with pain and quality of life in knee OA. Methods. Baseline data on 190 subjects with knee OA (mean +/- SD age 60.2 +/- 10.4 years, body mass index 32.7 +/- 7.2 kg/m(2)) were obtained from a randomized controlled trial. Knee pain was measured using the Western Ontario and McMaster Universities Osteoarthritis Index, and health-related quality of life was assessed using the Short Form 36 (SF-36). One-repetition maximum (1RM) strength was assessed using the bilateral leg press, and peak muscle power was measured during 5 maximum voluntary velocity repetitions at 40% and 70% of 1RM. Results. In univariate analysis, greater muscle power was significantly associated with pain (r = -0.17, P < 0.02) and also significantly and positively associated with SF-36 physical component summary (PCS) scores (r = 0.16, P < 0.05). After adjustment for multiple covariates, muscle power was a significant independent predictor of pain (P <= 0.05) and PCS scores (P <= 0.04). However, muscle strength was not an independent determinant of pain or quality of life (P >= 0.06). Conclusion. Muscle power is an independent determinant of pain and quality of life in knee OA. Compared to strength, muscle power may be a more clinically important measure of muscle function within this population. New trials to systematically examine the impact of muscle power training interventions on disease severity in knee OA are particularly warranted. C1 [Reid, Kieran F.; Price, Lori Lyn; Harvey, William F.; Driban, Jeffrey B.; Hau, Cynthia; Fielding, Roger A.; Wang, Chenchen] Tufts Univ, Boston, MA 02111 USA. [Price, Lori Lyn; Harvey, William F.; Driban, Jeffrey B.; Wang, Chenchen] Tufts Med Ctr, Boston, MA USA. RP Reid, KF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM kieran.reid@tufts.edu FU National Center for Complementary and Integrative Health at the NIH [R01-AT-005521, K24-AT-007323, UL1-RR-025752, UL1-TR-000073, UL1-TR-001064]; Boston Claude D. Pepper Older Americans Independence Center (through NIH) [P30-AG-031679]; Boston Rehabilitation Outcomes Center Grant (through NIH) [R24-HD-065688-01A1]; US Department of Agriculture [58-1950-4-003]; Nestle; Regeneron; Merck; Eli Lilly; Essentient; Dairy Management Inc.; Astellas; Cytokinetics; Pfizer; Pronutria Biosciences FX Supported by the National Center for Complementary and Integrative Health at the NIH (grants R01-AT-005521, K24-AT-007323, UL1-RR-025752, UL1-TR-000073, and UL1-TR-001064), the Boston Claude D. Pepper Older Americans Independence Center (through NIH grant P30-AG-031679), and the Boston Rehabilitation Outcomes Center Grant (through NIH grant R24-HD-065688-01A1). The research was also based upon work supported by the US Department of Agriculture under agreement 58-1950-4-003.; Dr. Fielding has received consulting fees and/or honoraria from Nestle, Regeneron, Merck, Eli Lilly, Essentient, Dairy Management Inc., Astellas, Cytokinetics, and Pfizer (less than $10,000 each) and from Pronutria Biosciences (more than $10,000); he owns stock or stock options in Ammonett Pharma, Segterra, MyoSyntax, and Pronutria Biosciences. NR 36 TC 2 Z9 2 U1 3 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2326-5191 EI 2326-5205 J9 ARTHRITIS RHEUMATOL JI Arthritis Rheumatol. PD DEC PY 2015 VL 67 IS 12 BP 3166 EP 3173 DI 10.1002/art.39336 PG 8 WC Rheumatology SC Rheumatology GA CX4SV UT WOS:000365691500010 PM 26315282 ER PT J AU Kern, CC Kenefic, LS Stout, SL AF Kern, Christel C. Kenefic, Laura S. Stout, Susan L. TI Bridging the Gender Gap: The Demographics of Scientists in the USDA Forest Service and Academia SO BIOSCIENCE LA English DT Article DE workforce diversity; inclusivity; natural resources; promotion; tenure ID WOMEN; SCIENCE; DIVERSIFICATION; ORGANIZATIONS; DEPARTMENTS; PERFORMANCE; CHALLENGES; KNOWLEDGE; PIPELINE; CAREERS AB Past research has established that diverse scientific communities foster innovation and problem solving more effectively than communities with a narrow range of knowledge, skills, and experience. However, gender diversity among scientists is limited, particularly in natural-resource fields. We compared data on scientist gender and rank from the US Department of Agriculture Forest Service Research and Development (FSR&D, a hierarchical organization) with data on faculty gender and tenure status from universities (loosely coupled systems) with comparable areas of study. We found that the representation of women was greater among FSR&D scientists than among university faculty but declined with seniority in both institutions. Within FSR&D, data showed demographic inertia, suggesting that the representation of women in senior scientist positions will increase. Although many mechanisms affect gender representation, our findings suggest that organizational structure affects the diversity of the scientific workforce. C1 [Kern, Christel C.] US Forest Serv, USDA, No Res Stn, Rhinelander, WI 54501 USA. [Kenefic, Laura S.] US Forest Serv, USDA, No Res Stn, Bradley, ME USA. [Stout, Susan L.] US Forest Serv, USDA, No Res Stn, Irvine, PA USA. RP Kern, CC (reprint author), US Forest Serv, USDA, No Res Stn, Rhinelander, WI 54501 USA. EM cckern@fs.fed.us FU USDA Forest Service, Northern Research Station, Civil Rights Special Project Fund FX We thank Cherie LeBlanc Fisher, Emma Schultz, and Cori Weldon for their technical assistance and Lynne Westphal, Laura Leites, and the four anonymous reviewers for their invaluable comments on earlier drafts of this manuscript. This project was funded by the USDA Forest Service, Northern Research Station, Civil Rights Special Project Fund. NR 53 TC 2 Z9 2 U1 1 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD DEC PY 2015 VL 65 IS 12 BP 1165 EP 1172 DI 10.1093/biosci/biv144 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CX6RN UT WOS:000365829200008 ER PT J AU Apostol, KG Dumroese, RK Pinto, JR Davis, AS AF Apostol, Kent G. Dumroese, R. Kasten Pinto, Jeremiah R. Davis, Anthony S. TI Response of conifer species from three latitudinal populations to light spectra generated by light-emitting diodes and high-pressure sodium lamps SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE PAR supplemental lighting; energy savings; gas exchange; light response curve; chlorophyll; container nursery ID CHLOROPHYLL FLUORESCENCE; MOUNTAIN HEMLOCK; ENGELMANN SPRUCE; SEEDLING GROWTH; WHITE SPRUCE; BLUE-LIGHT; CONTROLLING PHOTOPERIOD; EXTENDED PHOTOPERIOD; SUPPLEMENTAL LIGHT; NORWAY SPRUCE AB Light-emitting diode (LED) technology shows promise for supplementing photosynthetically active radiation (PAR) in forest nurseries because of the potential reduction in energy consumption and an ability to supply discrete wavelengths to optimize seedling growth. Our objective was to examine the effects of light spectra supplied by LED and traditional high-pressure sodium (HPS) lamps on growth and physiology of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) and Engelmann spruce (Picea engelmannii Parry ex Engelm.) seedlings. We used three latitudinal sources for each species: British Columbia (BC), Idaho (ID), and New Mexico (NM). Container seedlings were grown for 17 weeks in the greenhouse under an 18 h photoperiod of ambient solar light supplemented with light delivered from HPS or LED. In general, seedlings grown under LED had significantly greater growth, gas exchange rates, and chlorophyll contents than those seedlings grown under HPS. The growth and physiological responses to supplemental lighting varied greatly among species and seed sources. Generally, LED-grown seedlings from BC had the greatest growth and tissue dry mass followed by ID and NM populations. Compared with HPS, the significant increase in seedling growth and concomitant energy savings with LED (29% energy consumption relative to HPS) demonstrates the promise of using LED as PAR supplemental lighting for container seedling production. C1 [Apostol, Kent G.] Univ Arizona, Coll Agr & Life Sci, Cooperat Extens, Payson, AZ USA. [Dumroese, R. Kasten; Pinto, Jeremiah R.] USDA Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. [Davis, Anthony S.] Univ Idaho, Ctr Forest Nursery & Seedling Res, Moscow, ID 83843 USA. RP Apostol, KG (reprint author), Univ Arizona, Coll Agr & Life Sci, Cooperat Extens, Payson, AZ USA. EM kapostol@uidaho.edu FU USDA Forest Service Rocky Mountain Research Station; National Center for Reforestation, Nurseries, and Genetic Resources; University of Idaho, College of Natural Resources Center for Forest Nursery and Seedling Research FX This study was funded by the USDA Forest Service Rocky Mountain Research Station and the National Center for Reforestation, Nurseries, and Genetic Resources and the University of Idaho, College of Natural Resources Center for Forest Nursery and Seedling Research. We thank Don Regan, Brian Meyers, Craig Kingsbury, Lori Mackey, Jesse Paul, Garrett Hanson, Rebecca Sheridan, Layla Dunlap, and Kenneth Pete for filling containers and sowing and Katherine McBurney for greenhouse and technical support. We also thank Dr. Owen Burney and the USDA Forest Service Coeur d'Alene Nursery for providing seeds and David St. Germain for assistance in data collection. NR 60 TC 3 Z9 3 U1 6 U2 15 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD DEC PY 2015 VL 45 IS 12 BP 1711 EP 1719 DI 10.1139/cjfr-2015-0106 PG 9 WC Forestry SC Forestry GA CW9RJ UT WOS:000365335600005 ER PT J AU Looney, CE D'Amato, AW Palik, BJ Slesak, RA AF Looney, Christopher E. D'Amato, Anthony W. Palik, Brian J. Slesak, Robert A. TI Overstory treatment and planting season affect survival of replacement tree species in emerald ash borer threatened Fraxinus nigra forests in Minnesota, USA SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Fraxinus nigra; emerald ash borer; alternative tree species; artificial regeneration; canopy treatments ID BLACK ASH; NORTH-AMERICA; BOTTOMLAND RESTORATION; ASSISTED MIGRATION; WATER-TABLE; GROWTH; MORTALITY; INVASION; CONSEQUENCES; BIODIVERSITY AB Fraxinus nigra Marsh. (black ash) wetland forests in northern Minnesota, USA, are threatened by the invasive insect, emerald ash borer (Agrilus planipennis Fairmaire (EAB)). A potential management option is promoting regeneration of tree species that are not EAB hosts to maintain ecosystem functions. Using an operational-scale field experiment, we examined the survival of 12 alternative tree species in response to different canopy treatments. We planted the seedlings in 1.6 ha plots assigned to four replicated canopy treatments: untreated control, group selection (0.04 ha gaps, 20% of stand), black ash girdling to emulate EAB-induced mortality, and clearcut. Fall and spring plantings were used to compare the effects of spring ponding. Control (32.9%), group selection (34.5%), and girdling (33.3%) treatments had comparable overall seedling survival. Survival in the clear-cut treatments was significantly lower (22%). Species selection, overstory treatment, and season of planting together resulted in survival rates ranging from 0.08% to 94.1%. Conifer species had low overall rates of survival (10.7%), whereas some species with native ranges not presently overlapping with northern F. nigra forests, e.g., Quercus bicolor Willd. (75.5%), had high survival rates. If growth is light-limited, group selection may be effective in promoting recruitment and supporting a larger variety of species. C1 [Looney, Christopher E.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05095 USA. [Palik, Brian J.] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Slesak, Robert A.] Minnesota Forest Resources Council, St Paul, MN 51088 USA. RP Looney, CE (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM loone016@umn.edu FU Minnesota Environmental and Natural Resources Trust Fund; Frederick and Philip Noel Knorr and Northwest Paper Foundation Fellowships through the University of Minnesota, Department of Forest Resources; USDA Forest Service, Northern Research Station; Department of Interior Northeast Climate Science Center FX Funding was provided by the Minnesota Environmental and Natural Resources Trust Fund; the Frederick and Philip Noel Knorr and Northwest Paper Foundation Fellowships through the University of Minnesota, Department of Forest Resources; the USDA Forest Service, Northern Research Station; and the Department of Interior Northeast Climate Science Center. We thank Gary Swanson of the Chippewa National Forest for initially suggesting this project and the Chippewa National Forest staff who provided logistical support. Doug Kastendick of the USDA Forest Service, Northern Research Station, laid out harvest treatments and, along with Mike Reinikainen, Kyle Gill, and Nick Jensen of the University of Minnesota, assisted with tree planting. We are especially grateful to Mitch Slater of the Northern Research Station for coordinating timber sales and tree planting, maintaining measurement subplots, and tree survival data. Finally, we thank the field assistants who helped with planting and data collection. NR 56 TC 2 Z9 2 U1 14 U2 39 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD DEC PY 2015 VL 45 IS 12 BP 1728 EP 1738 DI 10.1139/cjfr-2015-0129 PG 11 WC Forestry SC Forestry GA CW9RJ UT WOS:000365335600007 ER PT J AU Chhin, S O'Brien, J AF Chhin, Sophan O'Brien, Joseph TI Dendroclimatic analysis of red pine affected by Diplodia shoot blight in different latitudinal regions in Michigan SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE climatic resiliency; dendrochronology; Diplodia pinea; forest pathology; Pinus resinosa; shoot blight; stand density ID LODGEPOLE PINE; SPHAEROPSIS-SAPINEA; CATASTROPHIC WIND; CLIMATE-CHANGE; GREAT-LAKES; TREE GROWTH; FOREST; SEEDLINGS; PRECIPITATION; TEMPERATURE AB Drought and intraspecific competition in red pine (Pinus resinosa Ait.) stands in Michigan have been implicated as predisposing factors for increased shoot blight risk caused by Diplodia pinea Desm. The overall objective of this study is to examine the interactive effects of drought, other climatic factors, and biotic factors (intraspecific competition) on productivity and growth of red pine forests affected by Diplodia shoot blight. This study incorporates a dendroclimatic approach that allows the retrospective examination of past annual diameter growth in relation to historical climate and the assessment of the potential impacts of future climate change. A total of 20 red pine stands were sampled in two latitudinal regions (the Upper Peninsula (UP) and the Lower Peninsula (LP) regions) of Michigan across two levels of initial stand density (low vs. high) and two levels of forest health condition (healthy vs. D. pinea affected). The full dendroclimatic relationships revealed in this study indicated that other climatic factors, in addition to summer drought stress, impacted red pine radial growth. Overall, red pine radial growth was generally more affected by precipitation and moisture index than solely by temperature variables. The radial growth response to climate depended on latitudinal region: summer moisture stress was more influential in the LP; cold spring and early summer temperatures negatively impacted growth more in the UP; and the degree of winter harshness was more of a factor in the UP. Crown damage caused by winter damage may have predisposed red pine stands to D. pinea affection by providing an easier entry point for fungal infection. Negative relations with precipitation in D. pinea affected stands may be due to increased dispersal of spores in D. pinea affected stands with significant rain and any increased storm and wind activity. Projections of radial growth under future scenarios of climate change indicate that climate warming has the potential to increase growth mainly in red pine stands in the UP region where growth has historically been limited by cool temperatures early in the growing season. Under the moisture index model, growth of red pine under the drier (A2) climate change scenario, only the D. pinea impacted high-density stands in the LP region are projected to show a significant decrease in growth by the middle (2041-2070) and final (2071-2100) projection periods. Model-based projections of forest growth in Michigan generally do not account for forest health issues such as invasive pathogens. The current study therefore provides a new understanding of the role of forest pathogens under future climate change. C1 [Chhin, Sophan] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [O'Brien, Joseph] USDA, Forest Serv, Forest Hlth Protect, St Paul, MN 55108 USA. RP Chhin, S (reprint author), Michigan State Univ, Dept Forestry, Nat Resources Bldg,480 Wilson Rd,Room 126, E Lansing, MI 48824 USA. EM chhin@msu.edu FU USDA Forest Service [11-DG-11420004-148]; Michigan State University (MSU) AgBioResearch [MICL06002] FX This study was supported by a grant from the USDA Forest Service (Forest Health Protection, Grant No. 11-DG-11420004-148) and through Michigan State University (MSU) AgBioResearch (Project No. MICL06002). We thank K. Lazda for his logistical support and GIS data for sampling Huron National Forest. We also thank D. Berry, E. David, A. Djoko, K. Finley, M. Magruder, K. Minnix, A. Monks, and M. Rooney for their assistance in field and laboratory data collection. NR 52 TC 0 Z9 0 U1 2 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD DEC PY 2015 VL 45 IS 12 BP 1757 EP 1767 DI 10.1139/cjfr-2015-0245 PG 11 WC Forestry SC Forestry GA CW9RJ UT WOS:000365335600010 ER PT J AU Worrall, JJ Keck, AG Marchetti, SB AF Worrall, James J. Keck, Andrew G. Marchetti, Suzanne B. TI Populus tremuloides stands continue to deteriorate after drought-incited sudden aspen decline SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE decline disease; climate change; regeneration; recent crown loss ID SOUTHWESTERN COLORADO; NORTH-AMERICA; WESTERN COLORADO; MORTALITY; FORESTS; LANDSCAPE; CLIMATE; EDGE; USA AB Reports of forest damage have increased with the frequency of climatic extremes, but longer term impacts of such events on population dynamics of forest trees are generally unknown. Incited by the turn-of-the-century drought, sudden aspen decline (SAD) damaged 535 000 ha of Populus tremuloides Michx. in the Southern Rockies ecoregion of western North America. Although spread of the disease stopped in about 2009, most of the affected stands continued to deteriorate. Remeasurement of plots in southwestern Colorado showed that, since the peak of the epidemic, live basal area in sick plots decreased by an additional 28% to only 38% of that in healthy plots. Sick plots had much more recent damage than healthy plots, with almost three times as much recently dead basal area, over twice the density of recently dead trees, and almost four times as much recent crown loss. The important contributing agents in SAD were still active in sick stands in 2013. Density of small regeneration showed opposite trends, increasing in healthy plots and decreasing in sick plots. Timely regeneration treatments may be needed in some such stands to facilitate recovery. In addition to acute damage from climatic extremes, long-term decline diseases like SAD will likely be a common signature of forest damage from climate change. C1 [Worrall, James J.; Marchetti, Suzanne B.] US Forest Serv, Forest Hlth Protect, Gunnison, CO 81230 USA. [Keck, Andrew G.] Western State Colorado Univ, Dept Math & Comp Sci, Gunnison, CO 81231 USA. RP Worrall, JJ (reprint author), US Forest Serv, Forest Hlth Protect, 216 N Colorado St, Gunnison, CO 81230 USA. EM jworrall@fs.fed.us NR 31 TC 0 Z9 0 U1 15 U2 19 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD DEC PY 2015 VL 45 IS 12 BP 1768 EP 1774 DI 10.1139/cjfr-2015-0225 PG 7 WC Forestry SC Forestry GA CW9RJ UT WOS:000365335600011 ER PT J AU Zhang, FF Liu, SS John, EM Must, A Demark-Wahnefried, W AF Zhang, Fang Fang Liu, Shanshan John, Esther M. Must, Aviva Demark-Wahnefried, Wendy TI Diet quality of cancer survivors and noncancer individuals: Results from a national survey SO CANCER LA English DT Article DE cancer survivors; diet quality; National Health and Nutrition Examination Survey; nutrition; quality of life ID INVASIVE BREAST-CANCER; PHYSICAL-ACTIVITY; HEALTH; PREVALENCE; FAMILY; IMPACT; WOMEN; RISK AB BACKGROUNDPatterns of poor nutritional intake may exacerbate the elevated morbidity experienced by cancer survivors. It remains unclear whether cancer survivors adhere to existing dietary guidelines and whether survivors' diets differ from those of individuals without cancer over the long term. METHODSThe authors evaluated dietary intake and quality in 1533 adult cancer survivors who participated in the National Health and Nutrition Examination Survey from 1999 to 2010 compared with dietary intake and quality in 3075 individuals who had no history of cancer and were matched to the cancer survivors by age, sex, and race/ethnicity. Dietary intake was assessed using 24-hour dietary recalls. The 2010 Healthy Eating Index (HEI-2010) was used to evaluate diet quality. RESULTSThe meanstandard deviation HEI-2010 total score was 47.20.5 in the cancer survivors and 48.3 +/- 0.4 in the noncancer group (P=.03). Compared with the noncancer group, cancer survivors had a significantly lower score for empty calories (13.6 vs 14.4; P=.001), which corresponded to worse adherence to dietary intake of calories from solid fats, alcohol, and added sugars. Cancer survivors also had significantly lower dietary intake of fiber than the noncancer group (15.0 vs 15.9 g per day; P=.02). In relation to recommended intake, survivors' mean dietary intake of vitamin D, vitamin E, potassium, fiber, and calcium was 31%, 47%, 55%, 60%, and 73%, respectively; whereas their mean dietary intake of saturated fat and sodium was 112% and 133%, respectively, of the recommended intake. CONCLUSIONSCancer survivors had poor adherence to the US Department of Agriculture 2010 Dietary Guidelines for Americans, and their intake patterns were worse than those in the general population for empty calories and fiber. Cancer 2015;121:4212-4221. (c) 2015 American Cancer Society. Cancer survivors in a national US survey have poor adherence to the 2010 dietary guidelines for Americans, and their intake patterns are worse than those in the general population for empty calories and fiber. Identifying nutritional patterns in cancer survivors is a priority for improving the survival and long-term health of this population. C1 [Zhang, Fang Fang; Liu, Shanshan] Tufts Univ, Friedman Sch Nutr Sci & Policy, Dept Nutr Sci, Boston, MA 20111 USA. [Zhang, Fang Fang] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. [John, Esther M.] Canc Prevent Inst Calif, San Francisco, CA USA. [Must, Aviva] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. [Demark-Wahnefried, Wendy] Univ Alabama Birmingham, Dept Nutr Sci, Birmingham, AL 35294 USA. RP Zhang, FF (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Dept Nutr Sci, 150 Harrison Ave, Boston, MA 20111 USA. EM fang_fang.zhang@tufts.edu FU Boston Nutrition Obesity Research Center [P30DK46200]; National Center for Research Resources [UL1 RR025752]; National Center for Advancing Translational Sciences; National Institutes of Health [UL1 TR000073]; Tufts Collaborates Grant FX All phases of this study were supported by a Boston Nutrition Obesity Research Center grant (P30DK46200), a grant from the National Center for Research Resources (UL1 RR025752), the National Center for Advancing Translational Sciences, the National Institutes of Health (UL1 TR000073), and Tufts Collaborates Grant. The funding sources had no role in the design, conduct, or analysis of this study or the decision to submit the article for publication. NR 27 TC 3 Z9 3 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0008-543X EI 1097-0142 J9 CANCER-AM CANCER SOC JI Cancer PD DEC 1 PY 2015 VL 121 IS 23 BP 4212 EP 4221 DI 10.1002/cncr.29488 PG 10 WC Oncology SC Oncology GA CX3TJ UT WOS:000365621900017 PM 26624564 ER PT J AU Chung, ST Chacko, SK Sunehag, AL Haymond, MW AF Chung, Stephanie T. Chacko, Shaji K. Sunehag, Agneta L. Haymond, Morey W. TI Measurements of Gluconeogenesis and Glycogenolysis: A Methodological Review SO DIABETES LA English DT Review ID HEPATIC GLUCOSE-PRODUCTION; ISOTOPOMER DISTRIBUTION ANALYSIS; NUCLEAR-MAGNETIC-RESONANCE; DOSE-RESPONSE CHARACTERISTICS; DEPENDENT DIABETES-MELLITUS; TOTAL PARENTERAL-NUTRITION; IMPAIRED FASTING GLUCOSE; MASS ISOTOPOMER; LIVER-GLYCOGEN; C-13 NMR AB Gluconeogenesis is a complex metabolic process that involves multiple enzymatic steps regulated by myriad factors, including substrate concentrations, the redox state, activation and inhibition of specific enzyme steps, and hormonal modulation. At present, the most widely accepted technique to determine gluconeogenesis is by measuring the incorporation of deuterium from the body water pool into newly formed glucose. However, several techniques using radioactive and stable-labeled isotopes have been used to quantitate the contribution and regulation of gluconeogenesis in humans. Each method has its advantages, methodological assumptions, and set of propagated errors. In this review, we examine the strengths and weaknesses of the most commonly used stable isotopes methods to measure gluconeogenesis in vivo. We discuss the advantages and limitations of each method and summarize the applicability of these measurements in understanding normal and pathophysiological conditions. C1 [Chung, Stephanie T.] NIDDKD, NIH, Bethesda, MD USA. [Chacko, Shaji K.; Sunehag, Agneta L.; Haymond, Morey W.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Haymond, MW (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM mhaymond@bcm.edu FU U.S. Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine; intramural department at the National Institute of Diabetes and Digestive and Kidney Diseases at the National Inititutes of Health; Marilyn Fishman Endocrine Fellows Foundation [2R01-HD-037957, 1R01-HD-044609, 5T32-DK-063873, 5R01-DK-055478]; U.S. Department of Agriculture [CRIS 6250-51000] FX This work is a publication of the U.S. Department of Agriculture/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine. S.T.C. is supported by the intramural department at the National Institute of Diabetes and Digestive and Kidney Diseases at the National Inititutes of Health. The authors' studies reported in this review were supported by the Marilyn Fishman Endocrine Fellows Foundation (grant to S.T.C.), 2R01-HD-037957 (A.L.S.), 1R01-HD-044609 (A.L.S.), 5T32-DK-063873 (M.W.H.), 5R01-DK-055478 (M.W.H.), and the U.S. Department of Agriculture (CRIS 6250-51000 to M.W.H.). NR 123 TC 2 Z9 2 U1 2 U2 13 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0012-1797 EI 1939-327X J9 DIABETES JI Diabetes PD DEC PY 2015 VL 64 IS 12 BP 3996 EP 4010 DI 10.2337/db15-0640 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CX8DN UT WOS:000365932900008 PM 26604176 ER PT J AU Arriola, CS Nelson, DI Deliberto, TJ Blanton, L Kniss, K Levine, MZ Trock, SC Finelli, L Jhung, MA AF Arriola, Carmen S. Nelson, Deborah I. Deliberto, Thomas J. Blanton, Lenee Kniss, Krista Levine, Min Z. Trock, Susan C. Finelli, Lyn Jhung, Michael A. CA H5 Invest Grp TI Infection Risk for Persons Exposed to Highly Pathogenic Avian Influenza A H5 Virus-Infected Birds, United States, December 2014-March 2015 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID POULTRY WORKERS; CHINA; OUTBREAKS; HUMANS; H7N9 AB Newly emerged highly pathogenic avian influenza (HPAI) A H5 viruses have caused outbreaks among birds in the United States. These viruses differ genetically from HPAI H5 viruses that previously caused human illness, most notably in Asia and Africa. To assess the risk for animal-to-human HPAI H5 virus transmission in the United States, we determined the number of persons with self-reported exposure to infected birds, the number with an acute respiratory infection (ARI) during a 10-day postexposure period, and the number with ARI who tested positive for influenza by real-time reverse transcription PCR or serologic testing for each outbreak during December 15, 2014 March 31, 2015. During 60 outbreaks in 13 states, a total of 164 persons were exposed to infected birds. ARI developed in 5 of these persons within 10 days of exposure. H5 influenza virus infection was not identified in any persons with ARI, suggesting a low risk for animal-to-human HPAI H5 virus transmission. C1 [Arriola, Carmen S.; Blanton, Lenee; Kniss, Krista; Levine, Min Z.; Trock, Susan C.; Finelli, Lyn; Jhung, Michael A.] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. [Nelson, Deborah I.] USDA, Washington, DC 20250 USA. [Deliberto, Thomas J.] USDA, Ft Collins, CO USA. RP Arriola, CS (reprint author), Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mailstop A32, Atlanta, GA 30329 USA. EM wus3@cdc.gov NR 25 TC 2 Z9 2 U1 0 U2 7 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD DEC PY 2015 VL 21 IS 12 BP 2135 EP 2140 DI 10.3201/eid2112.150904 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CX1MT UT WOS:000365461000004 PM 26583382 ER PT J AU Stokes, WS AF Stokes, W. S. TI Animals and the 3Rs in toxicology research and testing: The way forward SO HUMAN & EXPERIMENTAL TOXICOLOGY LA English DT Article DE Animal welfare; alternative methods; humane endpoints; refinement; reduction; replacement AB Despite efforts to eliminate the use of animals in testing and the availability of many accepted alternative methods, animals are still widely used for toxicological research and testing. While research using in vitro and computational models has dramatically increased in recent years, such efforts have not yet measurably impacted animal use for regulatory testing and are not likely to do so for many years or even decades. Until regulatory authorities have accepted test methods that can totally replace animals and these are fully implemented, large numbers of animals will continue to be used and many will continue to experience significant pain and distress. In order to positively impact the welfare of these animals, accepted alternatives must be implemented, and efforts must be directed at eliminating pain and distress and reducing animal numbers. Animal pain and distress can be reduced by earlier predictive humane endpoints, pain-relieving medications, and supportive clinical care, while sequential testing and routine use of integrated testing and decision strategies can reduce animal numbers. Applying advances in science and technology to the development of scientifically sound alternative testing models and strategies can improve animal welfare and further reduce and replace animal use. C1 [Stokes, W. S.] USDA, Anim & Plant Hlth Inspect, Anim Care, Raleigh, NC USA. RP Stokes, WS (reprint author), USDA APHIS Anim Care, 920 Main Campus Dr,Suite 200, Raleigh, NC 27606 USA. EM william.s.stokes@aphis.usda.gov NR 28 TC 3 Z9 3 U1 9 U2 21 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0960-3271 EI 1477-0903 J9 HUM EXP TOXICOL JI Hum. Exp. Toxicol. PD DEC PY 2015 VL 34 IS 12 BP 1297 EP 1303 DI 10.1177/0960327115598410 PG 7 WC Toxicology SC Toxicology GA CX5ME UT WOS:000365745000014 PM 26614819 ER PT J AU Pratt, AJ DiDonato, M Shin, DS Cabelli, DE Bruns, CK Belzer, CA Gorringe, AR Langford, PR Tabatabai, LB Kroll, JS Tainer, JA Getzoff, ED AF Pratt, Ashley J. DiDonato, Michael Shin, David S. Cabelli, Diane E. Bruns, Cami K. Belzer, Carol A. Gorringe, Andrew R. Langford, Paul R. Tabatabai, Louisa B. Kroll, J. Simon Tainer, John A. Getzoff, Elizabeth D. TI Structural, Functional, and Immunogenic Insights on Cu,Zn Superoxide Dismutase Pathogenic Virulence Factors from Neisseria meningitidis and Brucella abortus SO JOURNAL OF BACTERIOLOGY LA English DT Article ID SMALL-ANGLE SCATTERING; X-RAY-SCATTERING; ESCHERICHIA-COLI; HAEMOPHILUS-DUCREYI; ACTIVE-SITE; CU,ZN-SUPEROXIDE DISMUTASE; ELECTROSTATIC RECOGNITION; SALMONELLA-TYPHIMURIUM; MOLECULAR REPLACEMENT; PROTECTIVE IMMUNITY AB Bacterial pathogens Neisseria meningitidis and Brucella abortus pose threats to human and animal health worldwide, causing meningococcal disease and brucellosis, respectively. Mortality from acute N. meningitidis infections remains high despite antibiotics, and brucellosis presents alimentary and health consequences. Superoxide dismutases are master regulators of reactive oxygen and general pathogenicity factors and are therefore therapeutic targets. Cu,Zn superoxide dismutases (SODs) localized to the periplasm promote survival by detoxifying superoxide radicals generated by major host antimicrobial immune responses. We discovered that passive immunization with an antibody directed at N. meningitidis SOD (NmSOD) was protective in a mouse infection model. To define the relevant atomic details and solution assembly states of this important virulence factor, we report high-resolution and X-ray scattering analyses of NmSOD and of SOD from B. abortus (BaSOD). The NmSOD structures revealed an auxiliary tetrahedral Cu-binding site bridging the dimer interface; mutational analyses suggested that this metal site contributes to protein stability, with implications for bacterial defense mechanisms. Biochemical and structural analyses informed us about electrostatic substrate guidance, dimer assembly, and an exposed C-terminal epitope in the NmSOD dimer. In contrast, the monomeric BaSOD structure provided insights for extending immunogenic peptide epitopes derived from the protein. These collective results reveal unique contributions of SOD to pathogenic virulence, refine predictive motifs for distinguishing SOD classes, and suggest general targets for antibacterial immune responses. The identified functional contributions, motifs, and targets distinguishing bacterial and eukaryotic SOD assemblies presented here provide a foundation for efforts to develop SOD-specific inhibitors of or vaccines against these harmful pathogens. IMPORTANCE By protecting microbes against reactive oxygen insults, SODs aid survival of many bacteria within their hosts. Despite the ubiquity and conservation of these key enzymes, notable species-specific differences relevant to pathogenesis remain undefined. To probe mechanisms that govern the functioning of Neisseria meningitidis and Brucella abortus SODs, we used X-ray structures, enzymology, modeling, and murine infection experiments. We identified virulence determinants common to the two homologs, assembly differences, and a unique metal reservoir within meningococcal SOD that stabilizes the enzyme and may provide a safeguard against copper toxicity. The insights reported here provide a rationale and a basis for SOD-specific drug design and an extension of immunogen design to target two important pathogens that continue to pose global health threats. C1 [Pratt, Ashley J.; DiDonato, Michael; Shin, David S.; Bruns, Cami K.; Getzoff, Elizabeth D.] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA. [Pratt, Ashley J.; DiDonato, Michael; Shin, David S.; Bruns, Cami K.; Getzoff, Elizabeth D.] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA. [Pratt, Ashley J.; Shin, David S.; Tainer, John A.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA. [Cabelli, Diane E.] Brookhaven Natl Lab, Dept Chem, Upton, NY 11973 USA. [Belzer, Carol A.; Tabatabai, Louisa B.] Natl Anim Dis Ctr, Ruminant Dis & Immunol, Ames, IA USA. [Gorringe, Andrew R.] Publ Hlth England, Salisbury, Wilts, England. [Langford, Paul R.] Univ London Imperial Coll Sci Technol & Med, Dept Med, Paediat Sect, London, England. [Tainer, John A.] Univ Texas MD Anderson Canc Ctr, Dept Mol & Cellular Oncol, Houston, TX 77030 USA. RP Getzoff, ED (reprint author), Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA. EM edg@scripps.edu FU NIH [R01GM039345, T32AG000266, R01GM105404]; Robert A. Welch Distinguished Chair in Chemistry; George John and Sheilah Livanos Charitable Trust; NSF; Skaggs Institute for Chemical Biology; Canadian Institutes of Health Research; Integrated Diffraction Analysis Technologies (IDAT) program - Department of Energy [BL12.3.1]; U.S. Department of Energy [DE-AC02-98CH10886] FX This work was funded by NIH grant R01GM039345 (to E.D.G. and J.A.T.). J.A.T. is supported by a Robert A. Welch Distinguished Chair in Chemistry. Work in the Kroll laboratory was supported by The George John and Sheilah Livanos Charitable Trust. A.J.P. was supported in part through predoctoral NSF and Skaggs Institute for Chemical Biology fellowships and an NIH T32AG000266 postdoctoral training grant. M.D. was supported by a Canadian Institutes of Health Research postdoctoral fellowship.; We thank the beamline staff at the Stanford Synchrotron Radiation Laboratory (SSRL) for their help during data collection and for the use of the Structurally Integrated BiologY for Life Sciences (SIBYLS) beamline at the Advanced Light Source at Lawrence Berkeley National Laboratory. The SIBYLS beamline (BL12.3.1) is funded through the Integrated Diffraction Analysis Technologies (IDAT) program, supported by the Department of Energy and by NIH grant R01GM105404. Pulse radiolysis studies were carried out at the Center for Radiation Chemical Research, Brookhaven National Laboratory, which was supported under contract DE-AC02-98CH10886 with the U.S. Department of Energy and by its Division of Chemical Sciences, Office of Basic Energy Sciences. The contributions and technical assistance provided by Chiharu Hitomi, Andrew S. Arvai, Carey Kassman, and Zhujin Cao are greatly appreciated. NR 123 TC 1 Z9 1 U1 0 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 EI 1098-5530 J9 J BACTERIOL JI J. Bacteriol. PD DEC PY 2015 VL 197 IS 24 BP 3834 EP 3847 DI 10.1128/JB.00343-15 PG 14 WC Microbiology SC Microbiology GA CX3TX UT WOS:000365623400010 PM 26459556 ER PT J AU Woodbury, B Eigenberg, R Franz, TE AF Woodbury, Bryan Eigenberg, Roger Franz, Trenton E. TI Resistivity Arrays as an Early Warning System for Monitoring Runoff Holding Ponds SO JOURNAL OF ENVIRONMENTAL AND ENGINEERING GEOPHYSICS LA English DT Article ID SEAL FORMATION; MANURE; SOIL; SEEPAGE; CONDUCTIVITY; WATER AB Monitoring wells are installed to intercept contaminants inadvertently discharged from inground structures designed to retain salt-affected wastewaters; however, several difficulties with collection and data interpretation limit their effectiveness. Therefore, improved monitoring methods are needed. The objective of this study was to evaluate the effectiveness of resistivity array technology as an early warning system to monitor for unintended basin discharge. Subsurface resistivity arrays were installed at two Nebraska sites: a beef cattle feedyard located at the U.S. Meat Animal Research Center, Clay Center, Nebraska (FyA) and a commercial cattle feeding operation (FyB). Monitoring well data did not identify any unintended discharge events during the study period. However, the resistivity array (RA) system detected a discharge event that was localized in the non-saturated zone adjacent to the pond at FyB within one day following a precipitation event. Monitoring the unsaturated portion allows the RA system a capacity beyond traditional monitoring wells, which can only intercept discharge carried in groundwater. Also, the RA system effectively measured a larger area (i.e., a virtual curtain) compared to the point measure typical of monitoring wells. Therefore, RA technology provides broader coverage and is more tolerant to placement issues for intercepting discharge. Finally, the capacity to automate the RA system provides a means to continuously monitor unintended subsurface discharge from runoff holding ponds. This continuous monitoring system is more likely to detect discharge events than the bi-annual sampling typically required for monitoring wells. Automatic and continuous monitoring provides feedyard operators options to better manage environmental impacts associated with runoff holding ponds. C1 [Woodbury, Bryan; Eigenberg, Roger] USDA ARS, Nutr & Environm Management Res Unit, USMARC, Clay Ctr, NE 68933 USA. [Franz, Trenton E.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. RP Woodbury, B (reprint author), USDA ARS, Nutr & Environm Management Res Unit, USMARC, State Spur 18D, Clay Ctr, NE 68933 USA. EM bryan.woodbury@ars.usda.gov; roger.eigenberg@ars.usda.gov; tfranz2@unl.edu FU University of Nebraska; AgraTek Corp. FX The research was conducted in cooperation with the Nebraska Department of Environmental Quality, The Nebraska Cattlemen Association and with the support of University of Nebraska and AgraTek Corp. NR 30 TC 1 Z9 1 U1 2 U2 7 PU ENVIRONMENTAL ENGINEERING GEOPHYSICAL SOC PI DENVER PA 1720 SOUTH BELLAIRE, STE 110, DENVER, CO 80222-433 USA SN 1083-1363 J9 J ENVIRON ENG GEOPH JI J. Environ. Eng. Geophys. PD DEC PY 2015 VL 20 IS 4 BP 319 EP 335 DI 10.2113/JEEG20.4.319 PG 17 WC Geochemistry & Geophysics; Engineering, Geological SC Geochemistry & Geophysics; Engineering GA CX8DV UT WOS:000365933700004 ER PT J AU Qi, J Li, Y Yokoyama, W Majeed, H Masamba, KG Zhong, F Ma, JG AF Qi, Jing Li, Yue Yokoyama, Wallace Majeed, Hamid Masamba, Kingsley George Zhong, Fang Ma, Jianguo TI Cellulosic fraction of rice bran fibre alters the conformation and inhibits the activity of porcine pancreatic lipase SO JOURNAL OF FUNCTIONAL FOODS LA English DT Article DE Rice bran; Insoluble dietary fibre; Physicochemical properties; Lipase; Circular dichroism; Conformation ID BOVINE SERUM-ALBUMIN; DIETARY FIBER; FUNCTIONAL-PROPERTIES; HYDRATION PROPERTIES; BINDING INTERACTION; SUGARCANE BAGASSE; WHEAT BRAN; ADSORPTION; PROTEIN; METABOLISM AB A cellulosic rice bran insoluble dietary fibre (RBIDF) with different structures and physicochemical properties is shown to inhibit the activity of pancreatic lipase (PL) at different levels. The adsorption rate and saturation level to PL were greatest for RBIDF-2.0 (modified with 2.0% H2SO4 + 1.25% KOH), which had the highest specific surface area and oil holding capacity. The conformational deformation of unbound PL in the supernatant of PL-RBIDF confirmed by circular dichroism (CD) suggested that not only bound but also unbound PL activities were altered. Free energy calculations using data from fluorescence spectroscopy revealed that binding of PL to fibre depends primarily on electrostatic interactions, and the binding process is spontaneous and exothermic. This study indicates that dietary intake of cellulosic fractions of rice bran may be useful in reducing the bioavailability of dietary fat by altering the conformation and activity of PL that might be useful to control obesity. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Qi, Jing; Li, Yue; Majeed, Hamid; Masamba, Kingsley George; Zhong, Fang; Ma, Jianguo] Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. [Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 125 Program [2011BAD23B02, 2013AA102207]; NSFC [31171686]; Natural Science Foundation of Jiangsu Province [BK2012556]; 111 Project [B0702] FX The authors are grateful to Dayang Rice Company of China (Jiangsu, China) for the provision of the rice bran used in this study. This work was financially supported by National 125 Program 2011BAD23B02, 2013AA102207; NSFC 31171686; Natural Science Foundation of Jiangsu Province-BK2012556; 111 Project B0702. NR 45 TC 2 Z9 2 U1 8 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1756-4646 J9 J FUNCT FOODS JI J. Funct. Food. PD DEC PY 2015 VL 19 BP 39 EP 48 DI 10.1016/j.jff.2015.09.012 PN A PG 10 WC Food Science & Technology SC Food Science & Technology GA CX3XZ UT WOS:000365634100005 ER PT J AU Morales, P Cebadera-Miranda, L Camara, RM Reis, FS Barros, L Berrios, JD Ferreira, ICFR Camara, M AF Morales, Patricia Cebadera-Miranda, Laura Camara, Rosa M. Reis, Filipa S. Barros, Lillian Berrios, Jose De J. Ferreira, Isabel C. F. R. Camara, Montana TI Lentil flour formulations to develop new snack-type products by extrusion processing: Phytochemicals and antioxidant capacity SO JOURNAL OF FUNCTIONAL FOODS LA English DT Article DE Functional snacks; Fibre enriched pulse flours; Extrusion process; Antioxidants; Bioactivity ID CONTROLLED PRESSURE-DROP; PHENOLIC-COMPOUNDS; DIETARY SOURCES; FRUIT POWDERS; FIBER; BIOAVAILABILITY; COOKING; PEA; BIOACCESSIBILITY; POLYPHENOLS AB The effects of extrusion processing on fibre (soluble and insoluble), total available carbohydrates, tocopherols, organic acids, total phenolics, flavonols, hydroxycinnamic and hydroxybenzoic acids, as well as on the antioxidant capacity of different fibre-enriched lentil flours, were evaluated before and after extrusion process. Total dietary fibre was partially decreased after extrusion, which correlated with a significant increase in the soluble fibre fraction. gamma-tocopherol was the major isoform, before and after extrusion. Additionally, a marked decrease of 83-94% in total tocopherols content after extrusion was observed. Conversely, an increase in most polyphenolic fractions was found, probably due to the effect of extrusion in the hydrolysis of polyphenols bound to fibre and proteins, with an increase in antioxidant activity. Only flavonols presented an extensive decrease (62-82%) after treatment. The novel pulse-based flours, enriched with gluten-free soluble and insoluble fibres, provide snack-type products with a balanced nutritional and antioxidants composition. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Morales, Patricia; Cebadera-Miranda, Laura; Camara, Rosa M.; Camara, Montana] Univ Complutense Madrid, Fac Farm, Dept Nutr & Bromatol 2, E-28040 Madrid, Spain. [Reis, Filipa S.; Barros, Lillian; Ferreira, Isabel C. F. R.] Inst Politecn Braganca, ESA, Ctr Invest Montanha CIMO, P-5301855 Braganca, Portugal. [Berrios, Jose De J.] USDA ARS WRRC, Albany, CA 94710 USA. RP Camara, M (reprint author), Univ Complutense Madrid, Fac Farm, Dept Nutr & Bromatol 2, Pza Ramon y Cajal S-N, E-28040 Madrid, Spain. EM mcamara@ucm.es RI Ferreira, Isabel/E-8500-2013; Reis, Filipa /I-2093-2013; Morales Gomez, Patricia/L-9267-2013; Camara, Montana/A-3520-2009 OI Ferreira, Isabel/0000-0003-4910-4882; Reis, Filipa /0000-0002-9159-0530; Morales Gomez, Patricia/0000-0002-4963-2696; Camara, Montana/0000-0001-9232-0703 FU ALIMNOVA research group [UCM-951505/2012]; CIMO research centre [Pest-OE/AGR/UI0690/2014]; L. Barros' research contract ("Compromisso para a Ciencia") FX The authors are grateful to ALIMNOVA research group (UCM-951505/2012) and to the CIMO research centre (Pest-OE/AGR/UI0690/2014) for financial support, and L. Barros' research contract ("Compromisso para a Ciencia 2008"). NR 43 TC 2 Z9 2 U1 9 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1756-4646 J9 J FUNCT FOODS JI J. Funct. Food. PD DEC PY 2015 VL 19 BP 537 EP 544 DI 10.1016/j.jff.2015.09.044 PN A PG 8 WC Food Science & Technology SC Food Science & Technology GA CX3XZ UT WOS:000365634100050 ER PT J AU Olson, LE Oakleaf, RJ Squires, JR Wallace, ZP Kennedy, PL AF Olson, Lucretia E. Oakleaf, Robert J. Squires, John R. Wallace, Zachary P. Kennedy, Patricia L. TI NESTING PAIR DENSITY AND ABUNDANCE OF FERRUGINOUS HAWKS (BUTEO REGALIS) AND GOLDEN EAGLES (AQUILA CHRYSAETOS) FROM AERIAL SURVEYS IN WYOMING SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Ferruginous Hawk; Buteo regalis; Golden Eagle; Aquila chrysaetos; distance sampling; double-observer sampling; grassland ecosystems; nesting density; sagebrush steppe; stratified random sampling; survey efficiency ID ESTIMATING DETECTION PROBABILITY; DISTANCE SAMPLING SURVEYS; GREATER SAGE-GROUSE; LINE-TRANSECT; MARK-RECAPTURE; POPULATION-SIZE; CONSERVATION; SAGEBRUSH; COMBINATION; HABITATS AB Raptors that inhabit sagebrush steppe and grassland ecosystems in the western United States may be threatened by continued loss and modification of their habitat due to energy development, conversion to agriculture, and human encroachment. Actions to protect these species are hampered by a lack of reliable data on such basic information as population size and density. We estimated density and abundance of nesting pairs of Ferruginous Hawks (Buteo regalis) and Golden Eagles (Aquila chrysaetos) in sagebrush steppe and grassland regions of Wyoming, based on aerial line transect surveys of randomly selected townships. In 2010 and 2011, we surveyed 99 townships and located 62 occupied Ferruginous Hawk nests and 36 occupied Golden Eagle nests. We used distance sampling to estimate a nesting pair density of 94.7 km(2) per pair (95% CI: 69.9-139.8 km(2)) for Ferruginous Hawks, and 165.9 km(2) per pair (95% CI: 126.8-230.8 km(2)) for Golden Eagles. Our estimates were similar to or lower than those from other studies in similar locations in previous years; thus, we recommend continued monitoring to determine trends in nesting pair density over time. Additionally, we performed double-observer surveys on a subset of transects with a helicopter as the second observation aircraft. We estimated probability of detecting occupied nests from fixed-wing plane versus helicopter, as well as time and expense of each survey mode. Although observers surveying from helicopters were 1.19 and 1.12 times more likely to detect Ferruginous Hawk and Golden Eagle occupied nests, respectively, the helicopter survey was 4.55 times costlier due to longer flight time and the higher hourly costs. Thus, when systematically surveying large areas, we found cost and time of the helicopter surveys outweighed the increase in nest detection. C1 [Olson, Lucretia E.; Squires, John R.] USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Oakleaf, Robert J.] Wyoming Dept Game & Fish, Lander, WY 82520 USA. [Wallace, Zachary P.; Kennedy, Patricia L.] Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, Union, OR 97331 USA. [Wallace, Zachary P.; Kennedy, Patricia L.] Oregon State Univ, Dept Fisheries & Wildlife, Union, OR 97331 USA. RP Olson, LE (reprint author), USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. EM lucretiaolson@fs.fed.us FU U.S.D.A. Rocky Mountain Research Station; Bureau of Land Management; Wyoming Game and Fish Department; Wyoming Wildlife Heritage Foundation; PacifiCorp; Pathfinder Renewable Wind Energy, LLC; Wyoming Governor's Office FX Primary research support was provided by the U.S.D.A. Rocky Mountain Research Station, Bureau of Land Management, and Wyoming Game and Fish Department. Additional funding sources include Wyoming Wildlife Heritage Foundation; PacifiCorp; Pathfinder Renewable Wind Energy, LLC; and the Wyoming Governor's Office. Elizabeth Furtaw provided essential GIS support. Pilots Dave Savage (Savage Air Service, Inc.), Ken Overfield (Sky Aviation, Corp.), Dave Stinson (Sky Aviation, Corp.), and Neil Cadwell (Elkhorn Aviation, LLC) were pivotal in project completion. Bob Oakleaf served as primary observer in the helicopter, Zach Wallace and Matt Wilson as primary observers for fixed-wing aerial surveys of transects and leaders of the field crews, which included Roderick Lawrence, Jon Lipke, and Ray Buchheit. NR 74 TC 1 Z9 1 U1 3 U2 15 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD DEC PY 2015 VL 49 IS 4 BP 400 EP 412 PG 13 WC Ornithology SC Zoology GA CX7EO UT WOS:000365864900006 ER PT J AU Carlson, JE Piirto, DD Keane, JJ Gill, SJ AF Carlson, Jennifer E. Piirto, Douglas D. Keane, John J. Gill, Samantha J. TI ESTIMATING SITE OCCUPANCY AND DETECTION PROBABILITIES FOR COOPER'S AND SHARP-SHINNED HAWKS IN THE SOUTHERN SIERRA NEVADA SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Sharp-shinned Hawk; Accipiter striatus; Cooper's Hawk; Accipiter cooperii; detection probability; long-term monitoring; site occupancy; survey techniques ID NORTHERN GOSHAWKS; NEST SITES; VOCALIZATIONS; BROADCAST; CALLS AB Long-term monitoring programs that can detect a population change over time can be useful for managers interested in assessing population trends in response to forest management activities for a particular species. Such long-term monitoring programs have been designed for the Northern Goshawk (Accipiter gentilis), but not for the more elusive Sharp-shinned (A. striatus) and Cooper's hawks (A. cooperii). The objectives of this study were to (1) determine if it was possible to survey for these two Accipiters at the same time successfully using a new survey technique, and (2) estimate occupancy rate and detection probabilities for both species. We used broadcast surveys (BSM) to determine presence/absence for nesting Sharp-shinned and Cooper's hawks at Mountain Home Demonstration State Forest located in the southern Sierra Nevada mountain range in California. We surveyed 34 sampling units that were defined as the average home-range size (1000 m(2)) of the smallest target species, the Sharp-shinned Hawk. The sampling units were surveyed twice in 2003 and 3-4 times in 2004 during the breeding season. We used program PRESENCE to estimate detection probabilities and model occupancy rates for Sharp-shinned and Cooper's hawks. Our results indicated that the BSM using both Accipiter vocalizations in sequence was valuable for surveying both Sharp-shinned and Cooper's hawks. Proportions of the study area occupied for Sharp-shinned and Cooper's hawks were 0.25 (SE = 0.079), and 0.40 (SE = 0.098), respectively. The probabilities of detecting Cooper's and Sharp-shinned hawks at any given site were 0.56 (SE = 0.098) and 0.47 (SE = 0.086), respectively. There were no published occupancy estimates or detection probabilities in the literature to directly compare to our study. Because these species are elusive and difficult to survey, it is imperative future studies that address occupancy estimation for Sharp-shinned and Cooper's hawk incorporate detection probabilities into their model. Incorporating other variables into an occupancy model, such as habitat type, timber harvest, forest health, and/or climatic variables will also improve occupancy estimates. C1 [Carlson, Jennifer E.] Calif Polytech State Univ San Luis Obispo, Dept Nat Resources Management, San Luis Obispo, CA 93407 USA. [Piirto, Douglas D.; Gill, Samantha J.] Calif Polytech State Univ San Luis Obispo, Dept Nat Resources Management & Environm Sci, San Luis Obispo, CA 93407 USA. [Keane, John J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Sierra Nevada Res Ctr, Davis, CA 95618 USA. RP Carlson, JE (reprint author), Dept Fish & Wildlife, 601 Locust St, Redding, CA 96001 USA. EM jennifer.carlson@wildlife.ca.gov FU CalFire; Cal Poly State University Agriculture Research and Initiative (ARI) FX We thank the CalFire and the Cal Poly State University Agriculture Research and Initiative (ARI) grant for funding this project. Also a special thanks to Jose Medina, Alan Frame, and Megan Bidart from Mountain Home Demonstration State Forest for providing their expertise and knowledge of the study area. Also many thanks to field crew members: S. Littlefield, G. Cameron, S. Blake, D. Kahrs, A. Kavalunas, T. Larsen, and C. Michell. NR 23 TC 0 Z9 0 U1 4 U2 24 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD DEC PY 2015 VL 49 IS 4 BP 450 EP 457 PG 8 WC Ornithology SC Zoology GA CX7EO UT WOS:000365864900010 ER PT J AU Taylor, MM Bumanlag, LP Brown, EM Liu, CK AF Taylor, M. M. Bumanlag, L. P. Brown, E. M. Liu, Cheng-Kung TI BIOPOLYMERS PRODUCED FROM GELATIN AND CHITOSAN USING POLYPHENOLS SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID FISH GELATIN; SHELF-LIFE; FILMS; ACID; COMPOSITE; HYDROGEL; QUALITY AB Chitin, and its derivative chitosan, is an abundant waste product derived from crustaceans (e.g. crab). It has unique properties, which enable its use in, but not limited to, cosmetic, medical and food applications. Chitosan has recently been studied, in conjunction with other waste carbohydrates and proteins, for the purpose of making biopolymer products with unique functional properties. Furthermore use of renewable polyphenols to assist in these reactions is a topic of growing interest. In prior research, we investigated the use of polyphenols, specifically gallic acid and the vegetable tannins quebracho and tara, to modify gelatin. We improved the physical properties of gelatin and were able to demonstrate that these products could be used effectively as fillers. At present, gelatin is scarce and becoming increasingly more expensive so we produced biopolymers from modification of gelatin and whey using the vegetable tannin tara, and made products to augment the gelatin; we further demonstrated that the resulting product could be used as a filler for leather. This present study investigated the preparation of a biopolymer using gelatin and chitosan, modified with the vegetable tannin tara, to make products that could be used either in leather making process, e.g. as fillers or coatings or could have other potential uses such as in film-making or as flocculants. Optimal conditions necessary for polyphenols to react with gelatin and chitosan were determined and physical properties showed that unique products were produced. The gels were examined for thermal stability and for fluorescence. We thus demonstrated that gelatin/chitosan/tara products are feasible and that gelatin products could be supplemented using an inexpensive abundant waste product, chitosan. C1 [Taylor, M. M.; Bumanlag, L. P.; Brown, E. M.; Liu, Cheng-Kung] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Taylor, MM (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM maryann.taylor@ars.usda.gov NR 32 TC 0 Z9 0 U1 7 U2 11 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD DEC PY 2015 VL 110 IS 12 BP 392 EP 400 PG 9 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA CX6SP UT WOS:000365832100002 ER PT J AU Byrdwell, WC AF Byrdwell, William Craig TI The Updated Bottom Up Solution Applied to Atmospheric Pressure Photoionization and Electrospray Ionization Mass Spectrometry SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE APPI-MS; ESI-MS; Triacylglycerols; Regioisomers; Lipidomics ID PERFORMANCE LIQUID-CHROMATOGRAPHY; ENANTIOMERIC TRIACYLGLYCEROLS; DIETARY-SUPPLEMENT; VEGETABLE-OILS; FATTY-ACIDS; VITAMIN-D; TRIGLYCERIDES; MS AB The Updated Bottom Up Solution (UBUS) was recently applied to atmospheric pressure chemical ionization (APCI) mass spectrometry (MS) of triacylglycerols (TAG). This report demonstrates that the UBUS applies equally well to atmospheric pressure photoionization (APPI) MS and to electrospray ionization (ESI) MS. Critical Ratio 1 (CR1), the [MH](+)/I [DAG](+) pound or [MNH4](+)/I [DAG](+) pound ratio, does not exhibit the same strongly sigmoidal shape as it does by APCI-MS. CR1 varies more widely for APPI-MS than by APCI-MS, having a maximum value of 11.8, indicating a much greater effect of unsaturation on ion ratios in APPI-MS than APCI-MS. Critical Ratio 2, the [AA](+)/[AB](+) ratio for Type II TAG or [AC](+)/([AB](+)+[BC](+)) ratio for Type III TAG, allows quantification of regioisomers of TAG, and shows good agreement for APPI-MS to regioisomer quantification determined by APCI-MS. Critical Ratio 3, the [BC](+)/[AB](+) ratio for Type III TAG, reveals new trends relating the degree of unsaturation by APPI-MS, and shows that structural assignments made by ESI-MS are in good agreement to APCI-MS data. In addition to providing valuable structural information, the Critical Ratios also constitute a reduced data set that allows APPI-MS or ESI-MS mass spectra to be reconstructed when processed through the UBUS. Quantification by APPI-MS of vitamin D in the gelcaps gave values of 42.90 +/- A 0.83 mu g, or 1716 +/- A 33 international units, in good agreement with APCI-MS. C1 [Byrdwell, William Craig] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Byrdwell, WC (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM C.Byrdwell@ars.usda.gov FU USDA Agricultural Research Service FX The work of Dr. Robert Goldschmidt to conduct fatty acid methyl ester analysis by GC-FID is gratefully acknowledged. This work was supported by the USDA Agricultural Research Service. Mention or use of specific products or brands do not represent or imply endorsement by the USDA. NR 25 TC 1 Z9 1 U1 4 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD DEC PY 2015 VL 92 IS 11-12 BP 1533 EP 1547 DI 10.1007/s11746-015-2735-z PG 15 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CX5TJ UT WOS:000365765100001 ER PT J AU White, M Gambone, M Yen, H Arnold, J Harmel, D Santhi, C Haney, R AF White, Michael Gambone, Marilyn Yen, Haw Arnold, Jeff Harmel, Daren Santhi, Chinnasamy Haney, Richard TI Regional Blue and Green Water Balances and Use by Selected Crops in the US SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE modeling; water use; SWAT; blue water; green water ID VIRTUAL WATER; GROUNDWATER FOOTPRINT; CENTRAL VALLEY; NUTRIENT LOAD; UNITED-STATES; HIGH-PLAINS; RESOURCES; TRADE; PRODUCTIVITY; RIVER AB The availability of freshwater is a prerequisite for municipal development and agricultural production, especially in the arid and semiarid portions of the western United States (U.S.). Agriculture is the leading user of water in the U.S. Agricultural water use can be partitioned into green (derived from rainfall) and blue water (irrigation). Blue water can be further subdivided by source. In this research, we develop a hydrologic balance by 8-Digit Hydrologic Unit Code using a combination of Soil and Water Assessment Tool simulations and available human water use estimates. These data are used to partition agricultural groundwater usage by sustainability and surface water usage by local source or importation. These predictions coupled with reported agricultural yield data are used to predict the virtual water contained in each ton of corn, wheat, sorghum, and soybeans produced and its source. We estimate that these four crops consume 480km(3) of green water annually and 23km(3) of blue water, 12km(3) of which is from groundwater withdrawal. Regional trends in blue water use from groundwater depletion highlight heavy usage in the High Plains, and small pockets throughout the western U.S. This information is presented to inform water resources debate by estimating the cost of agricultural production in terms of water regionally. This research illustrates the variable water content of the crops we consume and export, and the source of that water. C1 [White, Michael; Arnold, Jeff; Harmel, Daren; Haney, Richard] ARS, USDA, Temple, TX 76502 USA. [Gambone, Marilyn; Yen, Haw; Santhi, Chinnasamy] Texas A&M Univ, AgriLife Res, Temple, TX 76502 USA. RP White, M (reprint author), ARS, USDA, 808 E Blackland Rd, Temple, TX 76502 USA. EM mike.white@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service FX This work was funded by the U.S. Department of Agriculture, Agricultural Research Service. NR 52 TC 2 Z9 2 U1 10 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD DEC PY 2015 VL 51 IS 6 BP 1626 EP 1642 DI 10.1111/1752-1688.12344 PG 17 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CX6FX UT WOS:000365797700011 ER PT J AU Mago, R Zhang, P Vautrin, S Simkova, H Bansal, U Luo, MC Rouse, M Karaoglu, H Periyannan, S Kolmer, J Jin, Y Ayliffe, MA Bariana, H Park, RF McIntosh, R Dolezel, J Berges, H Spielmeyer, W Lagudah, ES Ellis, JG Dodds, PN AF Mago, Rohit Zhang, Peng Vautrin, Sonia Simkova, Hana Bansal, Urmil Luo, Ming-Cheng Rouse, Matthew Karaoglu, Haydar Periyannan, Sambasivam Kolmer, James Jin, Yue Ayliffe, Michael A. Bariana, Harbans Park, Robert F. McIntosh, Robert Dolezel, Jaroslav Berges, Helene Spielmeyer, Wolfgang Lagudah, Evans S. Ellis, Jeff G. Dodds, Peter N. TI The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus SO NATURE PLANTS LA English DT Article ID POWDERY MILDEW; SHORT ARM; BARLEY; MLA; CHROMOSOME; SELECTION; UG99; SR31; 1R AB We identify the wheat stem rust resistance gene Sr50 (using physical mapping, mutation and complementation) as homologous to barley Mla, encoding a coiled-coil nucleotide-binding leucine-rich repeat (CC-NB-LRR) protein. We show that Sr50 confers a unique resistance specificity different from Sr31 and other genes on rye chromosome 1RS, and is effective against the broadly virulent Ug99 race lineage. Extensive haplotype diversity at the rye Sr50 locus holds promise for mining effective resistance genes. C1 [Mago, Rohit; Periyannan, Sambasivam; Ayliffe, Michael A.; Spielmeyer, Wolfgang; Lagudah, Evans S.; Ellis, Jeff G.; Dodds, Peter N.] CSIRO Agr, Canberra, ACT 2601, Australia. [Zhang, Peng; Bansal, Urmil; Karaoglu, Haydar; Bariana, Harbans; Park, Robert F.; McIntosh, Robert] Univ Sydney, Plant Breeding Inst, Narellan, NSW 2567, Australia. [Vautrin, Sonia; Berges, Helene] INRA CNRGV, F-31326 Castanet Tolosan, France. [Simkova, Hana; Dolezel, Jaroslav] Ctr Reg Hana Biotechnol & Agr Res, Inst Expt Bot, CZ-78371 Olomouc, Czech Republic. [Luo, Ming-Cheng] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Rouse, Matthew; Kolmer, James; Jin, Yue] Univ Minnesota, USDA, ARS Cereal Dis Lab, St Paul, MN 55108 USA. RP Dodds, PN (reprint author), CSIRO Agr, GPO Box 1600, Canberra, ACT 2601, Australia. EM peter.dodds@csiro.au RI Dodds, Peter/D-1181-2009; Periyannan, Sambasivam/C-1722-2014; Rouse, Matthew/G-5474-2011; Ellis, Jeffrey/A-1999-2010; Mago, Rohit/D-3927-2009 OI Dodds, Peter/0000-0003-0620-5923; FU Grains Research and Development Corporation [CSP00161, US00063]; National Program of Sustainability I [LO1204] FX This work was supported by funds provided through Grains Research and Development Corporation (CSP00161 and US00063). H.S. and J.D. are supported by the National Program of Sustainability I (award LO1204). We are grateful to T. Richardson, D. Bhatt, S. Louis, X. Xia (CSIRO Agriculture, Australia) H. Miah and S. Hoxha (The University of Sydney, Australia) for technical assistance. NR 20 TC 10 Z9 10 U1 6 U2 23 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD DEC PY 2015 VL 1 IS 12 AR 15186 DI 10.1038/NPLANTS.2015.186 PG 3 WC Plant Sciences SC Plant Sciences GA CX4XS UT WOS:000365704700005 PM 27251721 ER PT J AU Mitchell, PJ Cooper, C Dawson-Hughes, B Gordon, CM Rizzoli, R AF Mitchell, P. J. Cooper, C. Dawson-Hughes, B. Gordon, C. M. Rizzoli, R. TI Life-course approach to nutrition SO OSTEOPOROSIS INTERNATIONAL LA English DT Review DE Calcium; Life-course; Nutrition; Osteoporosis; Protein; Vitamin D ID RANDOMIZED CONTROLLED-TRIALS; VITAMIN-D SUPPLEMENTATION; BONE-MINERAL DENSITY; MATERNAL DIETARY PATTERNS; CALCIUM SUPPLEMENTATION; FRACTURE RISK; HIP FRACTURE; POSTMENOPAUSAL WOMEN; PROTEIN SUPPLEMENTS; 25-HYDROXYVITAMIN D AB This narrative review summarizes the role that nutrition plays in the development and maintenance of a healthy skeleton throughout the life-course. Nutrition has a significant influence on bone health throughout the life cycle. This narrative review summarizes current knowledge and guidance pertaining to the development and maintenance of a healthy skeleton. The primary objectives proposed for good bone health at the various stages of life are the following: Children and adolescents: achieve genetic potential for peak bone mass Adults: avoid premature bone loss and maintain a healthy skeleton Seniors: prevention and treatment of osteoporosis Findings from cohort studies, randomized controlled trials, systematic reviews and meta-analyses, in addition to current dietary guidelines, are summarized with the intention of providing clear nutritional guidance for these populations and pregnant women. C1 [Mitchell, P. J.] Synth Med NZ Ltd, Auckland, New Zealand. [Mitchell, P. J.] Univ Notre Dame Australia, Sydney, NSW, Australia. [Cooper, C.] Univ Oxford, Nuffield Dept Orthopaed Rheumatol & Musculoskelet, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. [Cooper, C.] Univ Southampton, Southampton Gen Hosp, MRC Lifecourse Epidemiol Unit, Southampton, Hants, England. [Dawson-Hughes, B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gordon, C. M.] Brown Univ, Alpert Med Sch, Hasbro Childrens Hosp, Div Adolescent Med, Providence, RI 02912 USA. [Gordon, C. M.] Brown Univ, Alpert Med Sch, Hasbro Childrens Hosp, Div Endocrinol, Providence, RI 02912 USA. [Rizzoli, R.] Univ Hosp Geneva, Div Bone Dis, Geneva, Switzerland. [Rizzoli, R.] Fac Med, Geneva, Switzerland. RP Cooper, C (reprint author), Univ Oxford, Nuffield Dept Orthopaed Rheumatol & Musculoskelet, NIHR Musculoskeletal Biomed Res Unit, Oxford, England. EM cc@mrc.soton.ac.uk OI Mitchell, Paul/0000-0002-7439-0369 FU Medical Research Council [MC_UP_A620_1014, MC_U147585819, MC_UU_12011/1] NR 130 TC 5 Z9 5 U1 2 U2 13 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD DEC PY 2015 VL 26 IS 12 BP 2723 EP 2742 DI 10.1007/s00198-015-3288-6 PG 20 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CX2GS UT WOS:000365515600001 PM 26412214 ER PT J AU Bischoff-Ferrari, HA Orav, JE Kanis, JA Rizzoli, R Schlogl, M Staehelin, HB Willett, WC Dawson-Hughes, B AF Bischoff-Ferrari, H. A. Orav, J. E. Kanis, J. A. Rizzoli, R. Schloegl, M. Staehelin, H. B. Willett, W. C. Dawson-Hughes, B. TI Comparative performance of current definitions of sarcopenia against the prospective incidence of falls among community-dwelling seniors age 65 and older SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE Community-dwelling seniors; Comparative performance; Falls; Prevalence; Sarcopenia ID SKELETAL-MUSCLE MASS; LOWER-EXTREMITY PERFORMANCE; ALTERNATIVE DEFINITIONS; FUNCTIONAL IMPAIRMENT; REFERENCE VALUES; ADULTS; WOMEN; RISK; MEN; RECOMMENDATIONS AB In this study, we compare the extent to which seven available definitions of sarcopenia and two related definitions predict the rate of falling. Our results suggest that the definitions of Baumgartner and Cruz-Jentoft best predict the rate of falls among sarcopenic versus non-sarcopenic community-dwelling seniors. Introduction The purpose of the study is to compare the extent to which seven available definitions of sarcopenia and two related definitions predict the prospective rate of falling. Methods We studied a cohort of 445 seniors (mean age 71 years, 45 % men) living in the community who were followed with a detailed fall assessment for 3 years. For comparing the rate of falls in sarcopenic versus non-sarcopenic individuals, we used multivariate Poisson regression analyses adjusting for gender and treatment (original intervention tested vitamin D plus calcium against placebo). Of the seven available definitions, three were based on low lean mass alone (Baumgartner, Delmonico 1 and 2) and four required both low muscle mass and decreased performance in a functional test (Fielding, Cruz-Jentoft, Morley, Muscaritoli). The two related definitions were based on low lean mass alone (Studenski 1) and low lean mass contributing to weakness (Studenski 2). Results Among 445 participants, 231 fell, sustaining 514 falls over the 3-year follow-up. The prospective rate of falls in sarcopenic versus non-sarcopenic individuals was best predicted by the Baumgartner definition based on low lean mass alone (RR = 1.54; 95% CI 1.09-2.18) with 11% prevalence of sarcopenia and the Cruz-Jentoft definition based on low lean mass plus decreased functional performance (RR = 1.82; 95 % CI 1.24-2.69) with 7.1 % prevalence of sarcopenia. Consistently, fall rate was non-significantly higher in sarcopenic versus non-sarcopenic individuals based on the definitions of Delmonico 1, Fielding, and Morley. Conclusion Among the definitions investigated, the Baumgartner definition and the Cruz-Jentoft definition had the highest validity for predicting the rate of falls. C1 [Bischoff-Ferrari, H. A.; Schloegl, M.] Univ Zurich Hosp, Dept Geriatr & Aging Res, CH-8091 Zurich, Switzerland. [Bischoff-Ferrari, H. A.; Schloegl, M.] Univ Zurich, Ctr Aging & Mobil, Zurich, Switzerland. [Orav, J. E.] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Kanis, J. A.] Univ Sheffield, Ctr Metab Bone Dis, Sheffield, S Yorkshire, England. [Rizzoli, R.] Univ Hosp Geneva, Serv Bone Dis, Geneva, Switzerland. [Rizzoli, R.] Fac Med, Geneva, Switzerland. [Staehelin, H. B.] Univ Basel, Dept Geriatr, Basel, Switzerland. [Willett, W. C.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Dawson-Hughes, B.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Bischoff-Ferrari, HA (reprint author), Univ Zurich Hosp, Dept Geriatr & Aging Res, Raemistr 101, CH-8091 Zurich, Switzerland. EM Heike.Bischoff@usz.ch FU Baugarten Foundation; International Foundation for the Promotion of Nutrition Research and Nutrition Education FX The study was funded by the Baugarten Foundation and the International Foundation for the Promotion of Nutrition Research and Nutrition Education. NR 49 TC 13 Z9 13 U1 1 U2 5 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD DEC PY 2015 VL 26 IS 12 BP 2793 EP 2802 DI 10.1007/s00198-015-3194-y PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CX2GS UT WOS:000365515600007 PM 26068298 ER PT J AU Sanz-Saez, A Heath, KD Burke, PV Ainsworth, EA AF Sanz-Saez, Alvaro Heath, Katy D. Burke, Patricia V. Ainsworth, Elizabeth A. TI Inoculation with an enhanced N-2-fixing Bradyrhizobium japonicum strain (USDA110) does not alter soybean (Glycine maxMerr.) response to elevated [CO2] SO PLANT CELL AND ENVIRONMENT LA English DT Article DE elevated carbon dioxide; free air CO2 enrichment; N-2 fixation; nodulation; rhizobia ID BIOLOGICAL NITROGEN-FIXATION; POPULATION GENETIC-STRUCTURE; NODULATED LEGUMINOUS PLANTS; ATMOSPHERIC CARBON-DIOXIDE; ENRICHMENT FACE; SYMBIOTIC EFFECTIVENESS; LIMITED PHOTOSYNTHESIS; HYDROGEN REACTIONS; RHIZOBIAL STRAIN; DOWN-REGULATION AB This study tested the hypothesis that inoculation of soybean (Glycine maxMerr.) with a Bradyrhizobium japonicum strain (USDA110) with greater N-2 fixation rates would enhance soybean response to elevated [CO2]. In field experiments at the Soybean Free Air CO2 Enrichment facility, inoculation of soybean with USDA110 increased nodule occupancy from 5% in native soil to 54% in elevated [CO2] and 34% at ambient [CO2]. Despite this success, inoculation with USDA110 did not result in greater photosynthesis, growth or seed yield at ambient or elevated [CO2] in the field, presumably due to competition from native rhizobia. In a growth chamber experiment designed to study the effects of inoculation in the absence of competition, inoculation with USDA110 in sterilized soil resulted in nodule occupation of >90%, significantly greater N-15(2) fixation, photosynthetic capacity, leaf N and total plant biomass compared with plants grown with native soil bacteria. However, there was no interaction of rhizobium fertilization with elevated [CO2]; inoculation with USDA110 was equally beneficial at ambient and elevated [CO2]. These results suggest that selected rhizobia could potentially stimulate soybean yield in soils with little or no history of prior soybean production, but that better quality rhizobia do not enhance soybean responses to elevated [CO2]. C1 [Sanz-Saez, Alvaro; Heath, Katy D.; Burke, Patricia V.; Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Sanz-Saez, Alvaro] Univ Basque Country UPV EHU, Dept Plant Biol & Ecol, Fac Pharm, Vitoria 01007, Alava, Spain. [Ainsworth, Elizabeth A.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Ainsworth, EA (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM lisa.ainsworth@ars.usda.gov FU Education, Linguistic Policy, and Education Department of the Basque Country, Spain FX We thank Kristen Bishop, Brad Dalsing, Robert Koester, Mike Masters, Anna Molineaux, Chris Montes, Brittany Nohl, Taylor Pederson, Kannan Puthuval, and Sang-In Shim for technical and field support, Craig Yendrek for helpful comments, and Professor Michael Sadowsky for providing B. japonicum USDA110. A.S.-S. was the recipient of a postdoctoral fellowship granted by the Education, Linguistic Policy, and Education Department of the Basque Country, Spain. NR 78 TC 0 Z9 0 U1 4 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD DEC PY 2015 VL 38 IS 12 BP 2589 EP 2602 DI 10.1111/pce.12577 PG 14 WC Plant Sciences SC Plant Sciences GA CX6EV UT WOS:000365794900010 PM 26012898 ER PT J AU Jimenez-Aguilar, DM Grusak, MA AF Jimenez-Aguilar, Dulce M. Grusak, Michael A. TI Evaluation of Minerals, Phytochemical Compounds and Antioxidant Activity of Mexican, Central American, and African Green Leafy Vegetables SO PLANT FOODS FOR HUMAN NUTRITION LA English DT Article DE Leafy vegetables; Minerals; Vitamin C; Phenolics; Flavonoids; Antioxidant activity ID NUTRIENT CONTENT; BOUND PHENOLICS; HERBS; CAPACITY; FOODS AB The green leafy vegetables Cnidoscolus aconitifolius and Crotalaria longirostrata are native to Mexico and Central America, while Solanum scabrum and Gynandropsis gynandra are native to Africa. They are consumed in both rural and urban areas in those places as a main food, food ingredient or traditional medicine. Currently, there is limited information about their nutritional and phytochemical composition. Therefore, mineral, vitamin C, phenolic and flavonoid concentration, and antioxidant activity were evaluated in multiple accessions of these leafy vegetables, and their mineral and vitamin C contribution per serving was calculated. The concentrations of Ca, K, Mg and P in these leafy vegetables were 0.82-2.32, 1.61-7.29, 0.61-1.48 and 0.27-1.44 mg/g fresh weight (FW), respectively. The flavonoid concentration in S. scabrum accessions was up to 1413 mu g catechin equivalents/g FW, while the highest antioxidant activities were obtained in C. longirostrata accessions (52-60 mu mol Trolox equivalents/g FW). According to guidelines established by the US Food and Drug Administration, a serving size (30 g FW) of C. longirostrata would be considered an excellent source of Mo (20 % or more of the daily value), and a serving of any of these green leafy vegetables would be an excellent source of vitamin C. Considering the importance of the minerals, phytochemicals and antioxidants in human health and their presence in these indigenous green leafy vegetables, efforts to promote their consumption should be implemented. C1 [Jimenez-Aguilar, Dulce M.; Grusak, Michael A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Grusak, MA (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates Ave, Houston, TX 77030 USA. EM mike.grusak@ars.usda.gov FU CONACYT Postdoctoral Fellowship [234764]; USDA Agricultural Research Service [58-6250-0-008] FX This research has been supported by CONACYT Postdoctoral Fellowship Grant No 234764 to DMJA and by the USDA Agricultural Research Service through Cooperative Agreement No. 58-6250-0-008 to MAG. Authors thank Chee-Ming Li, David Dworak and Gretchen Kroh for providing excellent technical assistance. The contents of this publication do not necessarily reflect the view or policies of the U.S. Department of Agriculture, nor does mention of trade names, commercial products or organizations imply endorsement by the U.S. government. NR 34 TC 1 Z9 1 U1 10 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-9668 EI 1573-9104 J9 PLANT FOOD HUM NUTR JI Plant Food Hum. Nutr. PD DEC PY 2015 VL 70 IS 4 BP 357 EP 364 DI 10.1007/s11130-015-0512-7 PG 8 WC Plant Sciences; Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Plant Sciences; Chemistry; Food Science & Technology; Nutrition & Dietetics GA CX2FI UT WOS:000365510900001 PM 26490448 ER PT J AU Ayaz, FA Colak, N Topuz, M Tarkowski, P Jaworek, P Seiler, G Inceer, H AF Ayaz, Faik Ahmet Colak, Nesrin Topuz, Mujgan Tarkowski, Petr Jaworek, Pavel Seiler, Gerald Inceer, Huseyin TI Comparison of Nutrient Content in Fruit of Commercial Cultivars of Eggplant (Solanum melongena L.) SO POLISH JOURNAL OF FOOD AND NUTRITION SCIENCES LA English DT Article DE eggplant; fatty acid; mineral; organic acid; amino acid; polyamine ID DIETARY POLYAMINES; AMINO-ACIDS; EXCHANGE CHROMATOGRAPHY; MINERAL-COMPOSITION; VARIABILITY; PHENOLICS; GROWTH; SUGARS AB Eggplant (Solanum melongena L.) is one of the most popular common major vegetable crops worldwide. This study evaluated the nutritional content of seven commercial eggplant fruits in terms of fatty acid, mineral, sugar, organic acid, amino acid and polyamine contents. The most abundant fatty acid was linoleic acid (range, 39.14-53.81%, ave. 45%), and the most abundant mineral was K (range, 1556.2-3171.6 mg/kg fw, ave. 2331.9). The major organic acid was malic acid (range, 129.87-387.01 mg/g fw, ave. 157.49), and the major sugar was fructose (range, 1242.81-1379.77 mg/100 g fw, ave. 1350.88). The major polyamine was putrescine (11.54 and 25.70 nmol/g fw, ave. 17.86), and the major amino acid was glutamine (148.4 and 298.75 mg/100 g fw, ave. 219.74). Overall, taking into account the export potential of eggplants, these results may contribute to further studies aiming to improve other nutrient-rich varieties of eggplant in breeding programs. C1 [Ayaz, Faik Ahmet; Colak, Nesrin; Topuz, Mujgan; Inceer, Huseyin] Karadeniz Tech Univ, Fac Sci, Dept Biol, TR-61080 Trabzon, Turkey. [Tarkowski, Petr; Jaworek, Pavel] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, Cent Labs, Olomouc 78371, Czech Republic. [Tarkowski, Petr; Jaworek, Pavel] Palacky Univ, Res Support Fac Sci, Olomouc 78371, Czech Republic. [Tarkowski, Petr] Crop Res Inst, Dept Genet Resources Vegetables Med & Special Pla, Ctr Reg Hana Biotechnol & Agr Res, Olomouc 78371, Czech Republic. [Seiler, Gerald] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Ayaz, FA (reprint author), Karadeniz Tech Univ, Fac Sci, Dept Biol, TR-61080 Trabzon, Turkey. EM faa@ktu.edu.tr OI Tarkowski, Petr/0000-0002-7581-7686 FU Research Fund of Karadeniz Technical University [2005.111.004.02]; Council of Higher Education of Turkey (CoHE); (Sustainable development of research in the Centre of Region Hana) from National Program of Sustainability I, Ministry of Education, Youth and Sports, Czech Republic [LO1204] FX Financial support was provided by the Research Fund of Karadeniz Technical University (Project No. No: 2005.111.004.02). The authors Faik Ahmet AYAZ and Huse-yin INCEER also greatly appreciate support from The Council of Higher Education of Turkey (CoHE). This work was also supported, in part, by grant No. LO1204 (Sustainable development of research in the Centre of Region Hana) from the National Program of Sustainability I, Ministry of Education, Youth and Sports, Czech Republic. The authors declare no conflict of interest NR 36 TC 1 Z9 1 U1 6 U2 19 PU DE GRUYTER OPEN LTD PI WARSAW PA BOGUMILA ZUGA 32A ST, 01-811 WARSAW, POLAND SN 1230-0322 EI 2083-6007 J9 POL J FOOD NUTR SCI JI Pol. J. food Nutr. Sci. PD DEC PY 2015 VL 65 IS 4 BP 251 EP 259 DI 10.1515/pjfns-2015-0035 PG 9 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CX1NV UT WOS:000365463800003 ER PT J AU Samaniuk, JR Scott, CT Root, TW Klingenberg, DJ AF Samaniuk, Joseph R. Scott, C. Tim Root, Thatcher W. Klingenberg, Daniel J. TI Effects of process variables on the yield stress of rheologically modified biomass SO RHEOLOGICA ACTA LA English DT Article DE Lignocellulosic biomass; Rheology; Rheological modifier; Mixing; Yield stress; Corn stover ID PULP FIBER SUSPENSIONS; HIGH-SOLIDS BIOMASS; CELLULOSE; POLYMERS; DISSOLUTION; SLURRIES; KINETICS; SORPTION; WOOD AB Additives that alter the rheology of lignocellulosic biomass suspensions were tested under conditions of variable pH, temperature, and solid concentration. The effects of certain ions, biomass type, and time after the addition of rheological modifier were also examined. Torque and vane rheometry were used to measure the yield stress of samples. It was found that the effectiveness of rheological modifiers depends on pH over a range of 1.5 to 6, biomass type, concentration of certain ions, and time after addition. The time-dependent properties of rheologically modified biomass are sensitive to the type of rheological modifier, and also to mixtures of these additives, which can result in unexpected behavior. We show that time-dependent rheology is not correlated with time-dependent changes of the water-soluble polymer (WSP) in the aqueous environment, such as slow polymer hydration, suggesting that time-dependent changes in the polymer-fiber interaction may play a more significant role. C1 [Samaniuk, Joseph R.; Root, Thatcher W.; Klingenberg, Daniel J.] Univ Wisconsin, Madison, WI 53706 USA. [Scott, C. Tim] USDA Forest Serv, Madison, WI 53726 USA. RP Samaniuk, JR (reprint author), Univ Wisconsin, 1415 Engn Dr, Madison, WI 53706 USA. EM jsamaniu@gmail.com; Klingen@engr.wisc.edu FU US Department of Agriculture (NRI) [2006-35504-17401]; US Department of Agriculture (AFRI) [2010-65504-20406] FX This project was supported by the US Department of Agriculture (NRI award number 2006-35504-17401 and AFRI award number 2010-65504-20406). NR 38 TC 0 Z9 0 U1 5 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0035-4511 EI 1435-1528 J9 RHEOL ACTA JI Rheol. Acta PD DEC PY 2015 VL 54 IS 11-12 BP 941 EP 949 DI 10.1007/s00397-015-0884-5 PG 9 WC Mechanics SC Mechanics GA CX5LQ UT WOS:000365743500006 ER PT J AU Castagneri, D Bottero, A Motta, R Vacchiano, G AF Castagneri, D. Bottero, A. Motta, R. Vacchiano, G. TI Repeated spring precipitation shortage alters individual growth patterns in Scots pine forests in the Western Alps SO TREES-STRUCTURE AND FUNCTION LA English DT Article DE Climate change; Competition; Crown; Decline; Pinus sylvestris; Tree ring ID DROUGHT-INDUCED MORTALITY; INNER ALPINE ENVIRONMENT; MIXED-CONIFER FORESTS; ALBUM SSP AUSTRIACUM; CLIMATE-CHANGE; RADIAL GROWTH; NORWAY SPRUCE; TREE-GROWTH; INTRASPECIFIC COMPETITION; COOCCURRING CONIFERS AB In the inner-Alpine Aosta Valley, severe growth reductions occurred in Scots pine stands during 1987-1993 and 2003-2005. Trees more sensitive to May precipitation exhibited stronger growth decline during these periods. Over the last decades, Scots pine (Pinus sylvestris L.) decline has involved large areas in the European Alps. Although the species is supposed to be drought resistant, increased temperatures and droughts are often indicated as predisposing causes of the decline. Nevertheless, the exact climate conditions that initiate the decline, and the reasons why they differentially affect individual trees, are largely unknown. Our aims were to identify climate constraints on Scots pine growth, and elucidate the effect of individual characteristics, such as tree age, size, crown condition, mistletoe occurrence, competition, and sensitivity to climate, on tree growth decline and recovery after repeated climatic stress. We analysed 232 trees in four second-growth stands located at 985-1350 m a.s.l. in the inner-Alpine Aosta Valley, NW Italy. Multi-year growth declines, occurred in 1987-1993 and 2003-2005, were related to repeated May precipitation shortage, while temperatures and summer precipitations played a minor role. Growth decline was stronger in the stand at lower elevation and with higher competition intensity. At the individual scale, trees more sensitive to May precipitation exhibited lower growth rates during both dry periods, but not a faster recovery. In the lower elevation stand, tree growth decline was significantly related to both crown transparency and mistletoe abundance, which was almost absent in the other stands. We conclude that future variations in the spring precipitation regime could threaten Scots pine more than warming per se, in inner-Alpine valleys around 1000-1400 m a.s.l. Still, different individual sensitivity to precipitation will likely result in patchy patterns of healthy and declining trees within the same stand. C1 [Castagneri, D.] Univ Padua, Dept TeSAF, I-35020 Legnaro, PD, Italy. [Bottero, A.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Bottero, A.] USDA, Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Motta, R.; Vacchiano, G.] Univ Turin, Dept DISAFA, I-10095 Grugliasco, TO, Italy. RP Castagneri, D (reprint author), Univ Padua, Dept TeSAF, Viale Univ 16, I-35020 Legnaro, PD, Italy. EM daniele.castagneri@unipd.it RI Motta, Renzo/B-5542-2008; OI Motta, Renzo/0000-0002-1631-3840; Bottero, Alessandra/0000-0002-0410-2675; Vacchiano, Giorgio/0000-0001-8100-0659 FU Fondazione CRT-Progetto Alfieri "Le foreste di protezione della Valle d'Aosta''; European Commission; Piedmont Region (Direzione Opere Pubbliche, Difesa del Suolo, Economia Montana e Foreste); Aosta Valley Region (Direzione Foreste); Canton Valais (Services des Forets et du Paysage)-Progetto EU Interreg IIIA Italy-Switzerland; University of Padua [D320.PRGR13001]; USDA Forest Service Northern Research Station, University of Minnesota; NE Climate Science Center-US Department of the Interior FX We thank Anna Battiston, Roberta Berretti, Fabio Meloni, Emanuele Lingua, Luana Giordano, Cinzia Saponeri, Enrico Mazzetto, and Matthias Dobbertin, whom sadly passed away in 2012, for all suggestions, assistance with data collection, and processing of tree ring samples. Funding and logistic support was provided by Fondazione CRT-Progetto Alfieri 2004-2005 "Le foreste di protezione della Valle d'Aosta'', European Commission, Piedmont Region (Direzione Opere Pubbliche, Difesa del Suolo, Economia Montana e Foreste), Aosta Valley Region (Direzione Foreste) and Canton Valais (Services des Forets et du Paysage)-Progetto EU Interreg IIIA 2000-2006 Italy-Switzerland "Le pinete delle vallate alpine: un elemento del paesaggio in mutazione''. D.C. was supported by the University of Padua (Research Project D320.PRGR13001, Senior Research Grants 2012). A.B. was supported by USDA Forest Service Northern Research Station, University of Minnesota, and NE Climate Science Center-US Department of the Interior. NR 86 TC 1 Z9 1 U1 4 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0931-1890 EI 1432-2285 J9 TREES-STRUCT FUNCT JI Trees-Struct. Funct. PD DEC PY 2015 VL 29 IS 6 BP 1699 EP 1712 DI 10.1007/s00468-015-1250-z PG 14 WC Forestry SC Forestry GA CX5AE UT WOS:000365711700006 ER PT J AU Eberhardt, TL Labbe, N So, CL Kim, K Reed, KG Leduc, DJ Warren, JM AF Eberhardt, Thomas L. Labbe, Nicole So, Chi-Leung Kim, Keonhee Reed, Karen G. Leduc, Daniel J. Warren, Jeffrey M. TI Effects of long-term elevated CO2 treatment on the inner and outer bark chemistry of sweetgum (Liquidambar styraciflua L.) trees SO TREES-STRUCTURE AND FUNCTION LA English DT Article DE Ash; Climate change; Extractives; Lignin; Phloem; Rhytidome ID RISING ATMOSPHERIC CO2; PRINCIPAL COMPONENT ANALYSIS; STEM WOOD PROPERTIES; CARBON-DIOXIDE; ENRICHMENT FACE; PELLET QUALITY; NORWAY SPRUCE; PICEA-ABIES; PINE BARK; RESPONSES AB Long-term exposure of sweetgum trees to elevated atmospheric CO (2) concentrations significantly shifted inner bark (phloem) and outer bark (rhytidome) chemical compositions, having implications for both defense and nutrient cycling. Changes in plant tissue chemistry due to increasing atmospheric carbon dioxide (CO2) concentrations have direct implications for tissue resistance to abiotic and biotic stress while living, and soil nutrient cycling when senesced as litter. Although the effects of elevated CO2 concentrations on tree foliar chemistry are well documented, the effects on tree bark chemistry are largely unknown. The objective of this study was to determine the effects of a long-term elevated CO2 treatment on the contents of individual elements, extractives, ash, lignin, and polysaccharide sugars of sweetgum (Liquidambar styraciflua L.) bark. Trees were harvested from sweetgum plots equipped with the Free-Air CO2 Enrichment (FACE) apparatus, receiving either elevated or ambient CO2 treatments over a 12-year period. Whole bark sections were partitioned into inner bark (phloem) and outer bark (rhytidome) samples before analysis. Principal component analysis, coupled with either Fourier transform infrared spectroscopy or pyrolysis-gas chromatography-mass spectrometry data, was also used to screen for differences. Elevated CO2 reduced the N content (0.42 vs. 0.35 %) and increased the C:N ratio (109 vs. 136 %) of the outer bark. For the inner bark, elevated CO2 increased the Mn content (470 vs. 815 mg kg(-1)), total extractives (13.0 vs. 15.6 %), and residual ash content (8.1 vs. 10.8 %) as compared to ambient CO2; differences were also observed for some hemicellulosic sugars, but not lignin. Shifts in bark chemistry can affect the success of herbivores and pathogens in living trees, and as litter, bark can affect the biogeochemical cycling of nutrients within the forest floor. Results demonstrate that increasing atmospheric CO2 concentrations have the potential to impact the chemistry of temperate, deciduous tree bark such as sweetgum. C1 [Eberhardt, Thomas L.; Reed, Karen G.; Leduc, Daniel J.; Warren, Jeffrey M.] USDA, Forest Serv, Southern Res Stn, Pineville, LA USA. [Labbe, Nicole; Kim, Keonhee] Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN USA. [So, Chi-Leung] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Warren, Jeffrey M.] Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN USA. [Warren, Jeffrey M.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. RP Eberhardt, TL (reprint author), USDA, Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM teberhardt@fs.fed.us RI Warren, Jeffrey/B-9375-2012 OI Warren, Jeffrey/0000-0002-0680-4697 FU U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC05-00OR22725] FX This material is based upon work supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, under contract DE-AC05-00OR22725. The authors are grateful to Fred J. Matt, USDA Forest Service, Forest Products Laboratory, for the lignin and sugar analyses; Joanne Childs and Holly Vander Stel at ORNL carried out the total phenolic content analysis. NR 61 TC 0 Z9 0 U1 2 U2 20 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0931-1890 EI 1432-2285 J9 TREES-STRUCT FUNCT JI Trees-Struct. Funct. PD DEC PY 2015 VL 29 IS 6 BP 1735 EP 1747 DI 10.1007/s00468-015-1254-8 PG 13 WC Forestry SC Forestry GA CX5AE UT WOS:000365711700009 ER PT J AU Enloe, HA Lockaby, BG Zipperer, WC Somers, GL AF Enloe, Heather A. Lockaby, B. Graeme Zipperer, Wayne C. Somers, Greg L. TI Urbanization effects on leaf litter decomposition, foliar nutrient dynamics and aboveground net primary productivity in the subtropics SO URBAN ECOSYSTEMS LA English DT Article DE Decomposition; Litter quality; Urbanization; Net primary productivity; Soil carbon; Forest ID PINUS-ELLIOTTII PLANTATIONS; ORGANIC-MATTER; PHOSPHORUS DYNAMICS; NITROGEN DYNAMICS; FOREST-FLOOR; URBAN SOILS; CARBON; GRADIENT; FLORIDA; GROWTH AB Urbanization can alter nutrient cycling. This research evaluated how urbanization affected nutrient dynamics in the subtropics. We established 17-0.04 ha plots in five different land cover types-slash pine (Pinus elliottii) plantations (n = 3), rural natural pine forests (n = 3), rural natural oak forests (n = 4), urban pine forests (n = 3) and urban oak forests (n = 4) in the Florida panhandle, a subtropical region that has experienced rapid urbanization. On each plot, we measured the decomposition of mixed species foliar litter, the nutrient release patterns in decomposing litter, foliar litter quality, and forest floor temperatures. Aboveground net primary productivity and soil carbon and nitrogen contents were also measured to characterize the carbon and nitrogen stocks and fluxes in the urban and rural sites. Litter decay rates, liter quality indices and nutrient release patterns in decomposing litter did not differ among urban and rural forests despite differences in forest floor temperatures between urban and rural sites. Urban forest floor temperatures are on average warmer by 0.63 A degrees C in the winter (p = 0.005) and tend to have a more narrow temperature range than those of the rural forested sites. Foliar mass was measured over an 82 week period that was characterized by drought, which may have masked an urbanization effect. Urban forest land covers had higher aboveground net primary productivity and foliar productivity compared to rural land covers. This increased input of foliar carbon is not reflected in statistically different forest floor or surface soil (0-7.5 cm) carbon contents between urban and rural sites. Understanding how drought interacts with other drivers of change in urban systems may be a necessary component of city specific ecological knowledge. C1 [Enloe, Heather A.] Univ Florida, Soil & Water Sci Dept, Gainesville, FL 32611 USA. [Lockaby, B. Graeme; Somers, Greg L.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Zipperer, Wayne C.] USDA Forest Serv, Southern Res Stn, Gainesville, FL 32611 USA. RP Enloe, HA (reprint author), Univ Florida, Soil & Water Sci Dept, POB 110290, Gainesville, FL 32611 USA. EM heather.enloe@ufl.edu FU USDA Forest Service [G00004859]; Center for Forest Sustainability at Auburn University FX Funding for this research was provided by the USDA Forest Service (grant number G00004859) and the Center for Forest Sustainability at Auburn University. We would like to thank Tate's Hell State Park and Jerry Pitts, Florida Fish and Wildlife Box R Wildlife Management Area, for access to study sites. We would also like to thank Joey Shaw for a review of an earlier version of the manuscript. Andrew Williams, USDA NRCS, provided critical knowledge of soil formation in the study area and assisted in field site selection. Robin Governo provided key assistance in the lab. Jennifer Trusty, Nancy Loewenstein, Lisa Samuelson and Curtis Hansen provided help with plant identification. We would also like to thank Russ Muntifering and John Lin for help with lignin analysis and Jake Blackstock, Megan Bloodworth, Andrew Parsons, Camilla Melanie Nova Costa and Catherine Justice for additional help in the field and lab. NR 56 TC 3 Z9 3 U1 15 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD DEC PY 2015 VL 18 IS 4 BP 1285 EP 1303 DI 10.1007/s11252-015-0444-x PG 19 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA CX6NK UT WOS:000365817600014 ER PT J AU Arjunan, NK Kadarkarai, M Kumar, S Pari, M Thiyagarajan, N Vincent, CT Barnard, DR AF Arjunan, Naresh Kumar Kadarkarai, Murugan Kumar, Shobana Pari, Madhiyazhagan Thiyagarajan, Nataraj Vincent, C. Thomas Barnard, Donald R. TI Factors influencing the spatial distribution of Anopheles larvae in Coimbatore District, Tamil Nadu, India SO ACTA TROPICA LA English DT Article DE ArcGIS; Anopheles; Larval density; Larval habitats; Mapping ID MALARIA; VECTOR; SURVEILLANCE; HABITATS; FRANCE AB Malaria causes extensive morbidity and mortality in humans and results in significant economic losses in India. The distribution of immature malaria-transmitting Anopheles mosquitoes was studied in 17 villages in Coimbatore District as a prelude to the development and implementation of vector control strategies that are intended to reduce the risk of human exposure to potentially infectious mosquitoes. Eight Anopheles species were recorded. The most numerous species were Anopheles vagus, Anopheles subpictus, and Anopheles hyrcanus. The location of mosquito development sites and the density of larvae in each village was evaluated for correlation with selected demographic, biologic, and land use parameters using remote sensing and geographic information systems (GIS) technology. We found the number of mosquito development sites in a village and the density of larvae in such sites to be positively correlated with human population density but not the surface area (km(2)) of the village. The number of mosquito development sites and the density of larvae in each site were not correlated. Data from this study are being used to construct a GIS-based mapping system that will enable the location of aquatic habitats with Anopheles larvae in the Coimbatore District, Tamil Nadu, India as target sites for the application of vector control. (C) 2015 Elsevier B.V. All rights reserved. C1 [Arjunan, Naresh Kumar] Periyar Univ, Sch Life Sci, Dept Zool, Salem 636011, India. [Kadarkarai, Murugan; Pari, Madhiyazhagan; Thiyagarajan, Nataraj] Bharathiar Univ, Sch Life Sci, Dept Zool, Coimbatore 641046, Tamil Nadu, India. [Kumar, Shobana] Sri GVG Visalakshi Coll Women, Dept Zool, Udumalpet, India. [Vincent, C. Thomas] Zonal Entomol Team, Dept Publ Hlth & Prevent Med, Coimbatore 641018, Tamil Nadu, India. [Barnard, Donald R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Arjunan, NK (reprint author), Periyar Univ, Sch Life Sci, Dept Zool, Salem 636011, India. EM nareshphd@gmail.com FU Department of Science and Technology, Science&Engineering Research Board (SERB), New Delhi, India [SERB/LS-1074/2013] FX We thank the Department of Science and Technology, Science&Engineering Research Board (SERB), New Delhi-110070, India for providing funds (Award Registration No. SERB/LS-1074/2013) to carry out the project. NR 36 TC 0 Z9 0 U1 2 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD DEC PY 2015 VL 152 BP 121 EP 130 DI 10.1016/j.actatropica.2015.09.007 PG 10 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA CW5SW UT WOS:000365057900017 PM 26364718 ER PT J AU Junnila, A Revay, EE Mueller, GC Kravchenko, V Qualls, WA Xue, RD Allen, SA Beier, JC Schlein, Y AF Junnila, Amy Revay, Edita E. Mueller, Gunter C. Kravchenko, Vasiliy Qualls, Whitney A. Xue, Rui-de Allen, Sandra A. Beier, John C. Schlein, Yosef TI Efficacy of attractive toxic sugar baits (ATSB) against Aedes albopictus with garlic oil encapsulated in beta-cyclodextrin as the active ingredient SO ACTA TROPICA LA English DT Article DE Culicidae; Aedes albopictus; Sugar feeding; ATSB; Mosquito control; Israel ID NONTARGET ORGANISMS; MOSQUITO-CONTROL; ALLIUM-SATIVUM; CULEX-PIPIENS; PHLEBOTOMUS-PAPATASI; LARVICIDAL ACTIVITY; LEISHMANIA-MAJOR; ADULT MOSQUITOS; MALARIA VECTORS; FLORIDA AB We tested the efficacy of attractive toxic sugar bait (ATSB) with garlic oil microencapsulated in beta-cyclodextrin as active ingredient against Aedes albopictus in suburban Haifa, Israel. Two three-acre gardens with high numbers of Ae. albopictus were selected for perimeter spray treatment with ATSB and ASB (bait containing no active ingredient). Baits were colored with food dye to verify feeding of the mosquitoes. The mosquito population was monitored by human landing catches and sweep net catches in the surrounding vegetation. Experiments lasted for 44 days. Treatment occurred on day 13. The mosquito population collapsed about 4 days after treatment and continued to drop steadily for 27 days until the end of the study. At the experimental site the average pre-treatment landing rate was 17.2 per 5 mins. Two days post-treatment, the landing rate dropped to 11.4, and continued to drop to an average of 2.6 during the following 26 days. During the same period, the control population was stable. Few sugar fed females (8-10%) approached a human bait and anthrone tests showed relatively small amounts of sugar within their crop/gut. Around 60-70 % of males caught near our human bait were sugar positive which may indicate that the males were feeding on sugar for mating related behavior. From the vegetation treated with the toxic bait, we recovered significantly fewer (about 10-14%) males and females stained by ATSB than at the ASB-treated control. This may indicate that the toxic baits alter the resting behavior of the poisoned mosquitoes within the vegetation. Almost no Ae. albopictus females (5.2 +/- 1.4) approached human bait after treatment with ATSB. It therefore appears that microencapsulated garlic oil is an effective pesticide against Ae. albopictus when used in an ATSB system. (C) 2015 Elsevier B.V. All rights reserved. C1 [Junnila, Amy; Mueller, Gunter C.; Schlein, Yosef] Hebrew Univ Jerusalem, Fac Med, Kuvin Ctr Study Infect & Trop Dis, Dept Microbiol & Mol Genet,IMRIC, IL-91120 Jerusalem, Israel. [Revay, Edita E.] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-34995 Haifa, Israel. [Kravchenko, Vasiliy] Tel Aviv Univ, Dept Zool, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel. [Qualls, Whitney A.; Beier, John C.] Univ Miami, Dept Publ Hlth Sci, Miller Sch Med, Miami, FL 33136 USA. [Xue, Rui-de] Anastasia Mosquito Control Dist, St Augustine, FL 32080 USA. [Allen, Sandra A.] USDA, Ctr Med & Vet Entomol, Gainesville, FL 32608 USA. RP Junnila, A (reprint author), Hebrew Univ Jerusalem, Fac Med, Kuvin Ctr Study Infect & Trop Dis, Dept Microbiol & Mol Genet,IMRIC, IL-91120 Jerusalem, Israel. EM amyjunnila@gmail.com FU NIAID NIH HHS [R01 AI100968] NR 42 TC 1 Z9 1 U1 3 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD DEC PY 2015 VL 152 BP 195 EP 200 DI 10.1016/j.actatropica.2015.09.006 PG 6 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA CW5SW UT WOS:000365057900028 PM 26403337 ER PT J AU Skory, CD Cote, GL AF Skory, Christopher D. Cote, Gregory L. TI Secreted expression of Leuconostoc mesenteroides glucansucrase in Lactococcus lactis for the production of insoluble glucans SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Glucansucrase; Dextransucrase; Dextran; Mutan; Insoluble polysaccharide ID CONTROLLED GENE-EXPRESSION; ACID BACTERIA; SYSTEM NICE; STREPTOCOCCUS-SOBRINUS; HETEROLOGOUS PROTEIN; ACCEPTOR REACTIONS; NRRL B-1118; NISIN; DEXTRAN; LACTOBACILLUS AB We expressed a glucansucrase, DsrI, from Leuconostoc mesenteroides that catalyzes formation of water-insoluble glucans from sucrose using a nisin-controlled gene expression system in Lactococcus lactis. These polymers have potential for production of biodegradable gels, fibers, and films. We optimized production of DsrI using several different background vectors, signal peptides, strains, induction conditions, and bioreactor parameters to increase extracellular accumulation. Optimal production of the enzyme utilized a high-copy plasmid, pMSP3535H3, which contains a nisin immunity gene, L. lactis LM0230, and bioreactors maintained at pH 6.0 to stabilize the enzyme. We were able to significantly improve growth using the lactic acid inhibitor heme and by continuous removal of lactic acid with anion exchange resins, but enzyme production was less than the controls. The recombinant enzyme under optimized conditions accumulated in the culture medium to approximately 380 mg/L, which was over 150-fold higher compared to the native L. mesenteroides strain. Methods are also included for purification of DsrI utilizing the glucan-binding domain of the enzyme. C1 [Skory, Christopher D.; Cote, Gregory L.] USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. RP Skory, CD (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Prod Technol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM chris.skory@ars.usda.gov NR 54 TC 1 Z9 1 U1 4 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD DEC PY 2015 VL 99 IS 23 BP 10001 EP 10010 DI 10.1007/s00253-015-6854-0 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CW7IL UT WOS:000365171600011 PM 26239071 ER PT J AU Guo, T Engel, BA Shao, G Arnold, JG Srinivasan, R Kiniry, JR AF Guo, Tian Engel, Bernard A. Shao, Gang Arnold, Jeffrey G. Srinivasan, Raghavan Kiniry, James R. TI Functional Approach to Simulating Short-Rotation Woody Crops in Process-Based Models SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Short-rotation woody crops; Hybrid poplar; Cottonwood; Process-based models; Biofuel production modeling ID RADIATION-USE EFFICIENCY; INTENSIVELY CULTURED POPULUS; WATER-QUALITY IMPACTS; SOUTHERN GREAT-PLAINS; ESTABLISHMENT YEAR; CONTRASTING MORPHOLOGY; BIOMASS ACCUMULATION; LEAF WEIGHT; POPLAR; SWITCHGRASS AB Short-rotation woody crops (SRWCs) such as Populus have great potential as biofuel feedstocks. Biomass yields and yield stability at potential sites are important considerations when SRWCs are widely planted. The process-based, daily time-step simulation model Agricultural Land Management Alternative with Numerical Assessment Criteria (ALMANAC) offers promise as a useful tool to evaluate tree growth over large ranges of conditions. The objective of this study was to develop algorithms and growth parameters of hybrid poplar 'Tristis #1' (Populus balsamifera L. x Populus tristis Fisch) and eastern cottonwood (Populus deltoides Bartr.) in ALMANAC and to improve simulation of leaf area index (LAI) and plant biomass as well as biomass partitioning. ALMANAC with the improved algorithms for LAI and weight of falling leaves was applied to hybrid poplar plots in Wisconsin and cottonwood plots in Mississippi, and the modeled biomass yield and LAI were compared with measured data to modify and evaluate the location-specific ALMANAC models. Improved algorithms for LAI and biomass simulation and suggested values and potential parameter ranges for hybrid poplar and cottonwood were reasonable (Nash-Sutcliffe model efficiency (NSE) 0.81 +/- 0.99 and R (2) 0.76 +/- 0.99). ALMANAC with modified algorithms and parameters for Populus growth realistically simulated LAI, aboveground woody biomass, and root biomass of Populus. Thus, this model can be used for biofeedstock production modeling for Populus. The improved algorithms of LAI and biomass simulation for tree growth should also be useful for other process-based models, such as Soil and Water Assessment Tool (SWAT), Environmental Policy Integrated Climate (EPIC), and Agricultural Policy/Environmental eXtender (APEX). C1 [Guo, Tian; Engel, Bernard A.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Shao, Gang] Purdue Univ, Dept Forest & Nat Resources, W Lafayette, IN 47907 USA. [Arnold, Jeffrey G.; Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, Coll Stn, Temple, TX 76502 USA. RP Guo, T (reprint author), Purdue Univ, Dept Agr & Biol Engn, 225 South Univ St, W Lafayette, IN 47907 USA. EM guo190@purdue.edu RI Srinivasan, R/D-3937-2009 NR 59 TC 1 Z9 1 U1 1 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD DEC PY 2015 VL 8 IS 4 BP 1598 EP 1613 DI 10.1007/s12155-015-9615-0 PG 16 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CW5HP UT WOS:000365026600013 ER PT J AU Lowry, DB Taylor, SH Bonnette, J Aspinwall, MJ Asmus, AL Keitt, TH Tobias, CM Juenger, TE AF Lowry, David B. Taylor, Samuel H. Bonnette, Jason Aspinwall, Michael J. Asmus, Ashley L. Keitt, Tim H. Tobias, Christian M. Juenger, Thomas E. TI QTLs for Biomass and Developmental Traits in Switchgrass (Panicum virgatum) SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Biomass; Genetics; Linkagemap; Panicum virgatum; QTL ID EST-SSR MARKERS; GENETIC DIVERSITY; LINKAGE MAPS; ECOTYPES; ARABIDOPSIS; EXPRESSION; LOWLAND; ETHANOL; UPLAND; YIELD AB Genetic and genomic resources have recently been developed for the bioenergy crop switchgrass (Panicum virgatum). Despite these advances, little research has been focused on identifying genetic loci involved in natural variation of important bioenergy traits, including biomass. Quantitative trait locus (QTL) mapping is typically used to discover loci that contribute to trait variation. Once identified, QTLs can be used to improve agronomically important traits through marker-assisted selection. In this study, we conducted QTL mapping in Austin, TX, USA, with a full-sib mapping population derived from a cross between tetraploid clones of two major switchgrass cultivars (Alamo-A4 and Kanlow-K5). We observed significant among-genotype variation for the vast majority of growth, morphological, and phenological traits measured on the mapping population. Overall, we discovered 27 significant QTLs across 23 traits. QTLs for biomass production colocalized on linkage group 9b across years, as well as with a major biomass QTL discovered in another recent switchgrass QTL study. The experiment was conducted under a rainout shelter, which allowed us to examine the effects of differential irrigation on trait values. We found very minimal effects of the reduced watering treatment on traits, with no significant effect on biomass production. Overall, the results of our study set the stage for future crop improvement through marker-assisted selection breeding. C1 [Lowry, David B.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Taylor, Samuel H.] Bowdoin Coll, Dept Biol, Brunswick, ME 04011 USA. [Bonnette, Jason; Keitt, Tim H.; Juenger, Thomas E.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [Bonnette, Jason; Keitt, Tim H.; Juenger, Thomas E.] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. [Aspinwall, Michael J.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Asmus, Ashley L.] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. [Tobias, Christian M.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Lowry, DB (reprint author), Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. EM dlowry@msu.edu RI Tobias, Christian/B-6602-2009; OI Tobias, Christian/0000-0002-7881-750X; Taylor, Samuel/0000-0001-9714-0656 FU University of Texas Freshman Research Initiative; National Science Foundation [IOS-0922457]; US Department of Agriculture National Institute of Food and Agriculture-Agriculture and Food Research Initiative [2011-67012-30696]; California State University, Monterey Bay; Michigan State University FX We would like to especially thank the many volunteers and students that helped to conduct the fieldwork for this project. We also are grateful to John Crutchfield, who made the experiment possible. Charles Swanson (Texas A&M) designed our irrigation system and along with Guy Fipps (Texas A&M) developed our differential irrigation treatment plan. The University of Texas Freshman Research Initiative provided funding for training of undergraduate students that worked on this project. The National Science Foundation provided funding through a Plant Genome Research Program Award (IOS-0922457) to TJ. A US Department of Agriculture National Institute of Food and Agriculture-Agriculture and Food Research Initiative Postdoctoral Fellowship (2011-67012-30696) supported DL during the time that the experiment was conducted. California State University, Monterey Bay, and Michigan State University provided financial support to DL during the period of data analysis and writing of the manuscript. NR 38 TC 5 Z9 5 U1 2 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD DEC PY 2015 VL 8 IS 4 BP 1856 EP 1867 DI 10.1007/s12155-015-9629-7 PG 12 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CW5HP UT WOS:000365026600036 ER PT J AU Steffan, SA Chikaraishi, Y Horton, DR Miliczky, E Zalapa, JE Jones, VP Ohkouchi, N AF Steffan, Shawn A. Chikaraishi, Yoshito Horton, David R. Miliczky, Eugene Zalapa, Juan E. Jones, Vincent P. Ohkouchi, Naohiko TI Beneficial or not? Decoding carnivore roles in plant protection SO BIOLOGICAL CONTROL LA English DT Article DE N-15; Compound-specific; CSIA; Intraguild predation; Omnivore; Predator; Stable isotope fingerprinting; Trophic function ID INTRAGUILD PREDATION; FOOD WEBS; LABORATORY EXPERIMENTS; ISOTOPIC ECOLOGY; STABLE-ISOTOPES; AMINO-ACIDS; ASSUMPTIONS; DIVERSITY; OMNIVORY; INSECTS AB Carnivores indirectly protect plants by reducing herbivory. This important ecosystem service can be undermined, however, as carnivores feed upon fellow carnivores. Such intraguild predation is exceedingly common, yet measurement of the degree to which this occurs has remained elusive due to difficulties in measuring the trophic tendencies of free-roaming animals. Conventional molecular methods, such as bulk-isotopic analyses, do not produce reliably accurate trophic position estimates, and often the inaccuracy is substantial. With the advent of compound-specific isotopic analysis (CSIA), it is now possible to accurately quantify the lifetime trophic tendencies of wild carnivore populations. Unfortunately, CSIA is extraordinarily expensive and time-consuming, limiting the number of samples that can be analyzed. The need for high-quality trophic information has to be balanced with the inaccessibility of CSIA. Here, we propose coupling CSIA-derived trophic position estimates with conventional bulk-N-15 analysis, effectively calibrating site-specific bulk-N-15 data and thereby allowing for trophic position estimation using bulk data alone. We also create a framework that uses trophic position as a basis to characterize carnivores as beneficial for crop protection. Within an agricultural field, we demonstrated the utility of this new approach by measuring the trophic positions of six common arthropod species. We then compare these trophic position estimates to those deriving from conventional bulk-N-15 analysis. Our hybrid approach produced more accurate trophic position estimates than the stand-alone bulk-N-15 method. Ultimately, we were able to examine enough specimens to determine which carnivore populations were likely beneficial for plant protection, and which were not. Published by Elsevier Inc. C1 [Steffan, Shawn A.; Zalapa, Juan E.] USDA ARS, Madison, WI USA. [Steffan, Shawn A.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Chikaraishi, Yoshito; Ohkouchi, Naohiko] Japan Agcy Marine Earth Sci & Technol, Dept Biogeochem, Yokosuka, Kanagawa 2370061, Japan. [Horton, David R.; Miliczky, Eugene] USDA ARS, Yakima Agr Res Lab, Wapato, WA USA. [Jones, Vincent P.] Washington State Univ, Fruit Res & Extens Ctr, Dept Entomol, Wenatchee, WA USA. RP Steffan, SA (reprint author), Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM steffan@entomology.wisc.edu FU USDA-ARS [3655-21220-001, 5352-22430-001] FX This project was supported by USDA-ARS appropriated funds (3655-21220-001 and 5352-22430-001). Assistance with rearing and curating specimens was provided by M. Singleton, S. Horn, E. Wiesman, and C. Baker. Gratitude is extended to Drs. H. Gaines-Day, E. Chasen, and W. Snyder for helpful reviews of early manuscript drafts. NR 40 TC 0 Z9 0 U1 5 U2 25 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2015 VL 91 BP 34 EP 41 DI 10.1016/j.biocontrol.2015.07.002 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CW2EA UT WOS:000364802700006 ER PT J AU Layman, ML Lundgren, JG AF Layman, Marissa L. Lundgren, Jonathan G. TI Mutualistic and antagonistic trophic interactions in canola: The role of aphids in shaping pest and predator populations SO BIOLOGICAL CONTROL LA English DT Article DE Myzus persicae; Predation; Canola; Diversity; Community; Oilseed rape ID LIFE-HISTORY TRAITS; ENEMY-FREE SPACE; HOST-PLANT; INSECT HERBIVORES; SUGAR COMPOSITION; PIERIS-RAPAE; ANTS; HOMOPTERA; HONEYDEW; DNA AB Aphids have important effects on the abundance and occurrence of tending ants, predators, and pests in agronomic systems, and DNA-based gut content analysis can aid in establishing predator-prey interactions. The purpose of this study was to determine how the presence of aphids, ants, and pest individuals interact within canola (syn. oilseed rape) fields. Using seasonal data from canola fields, the relationships among ants, aphids and pest individuals were determined, along with the use of PCR techniques in order to amplify aphid DNA and confirm food web links on predators who consume aphids. We determined that aphid presence positively influences the number of ants and predators in a community, and diminishing aphid populations over the growing season were associated with declines in both ants and predators. These reduced populations of predators and aphids may have provided the opportunity for a key pest, Pieris rapae to build populations as the season ensued. This research suggests that complex interactions among herbivores and shared predators contribute to pest outbreaks. Published by Elsevier Inc. C1 [Layman, Marissa L.] S Dakota State Univ, Biol Microbiol Dept, Brookings, SD 57007 USA. [Lundgren, Jonathan G.] USDA ARS, NCARL, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM jonathan.lundgren@ars.usda.gov NR 61 TC 1 Z9 1 U1 4 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD DEC PY 2015 VL 91 BP 62 EP 70 DI 10.1016/j.biocontro1.2015.07.008 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CW2EA UT WOS:000364802700010 ER PT J AU Samac, DA Bucciarelli, B Miller, SS Yang, SS O'Rourke, JA Shin, S Vance, CP AF Samac, Deborah A. Bucciarelli, Bruna Miller, Susan S. Yang, S. Samuel O'Rourke, Jamie A. Shin, Sanghyun Vance, Carroll P. TI Transgene silencing of sucrose synthase in alfalfa (Medicago sativa L.) stem vascular tissue suggests a role for invertase in cell wall cellulose synthesis SO BMC PLANT BIOLOGY LA English DT Article DE Biofuels; Cell wall biosynthesis; Cellulose; Gene silencing; Phloem; Xylem ID MEMBRANE H+-ATPASE; CYTOSOLIC INVERTASE; STATISTICAL-MODEL; LOTUS-JAPONICUS; NORMAL GROWTH; ROOT-NODULES; GENE; LOCALIZATION; PLANTS; MAIZE AB Background: Alfalfa (Medicago sativa L.) is a widely adapted perennial forage crop that has high biomass production potential. Enhanced cellulose content in alfalfa stems would increase the value of the crop as a bioenergy feedstock. We examined if increased expression of sucrose synthase (SUS; EC 2.4.1.13) would increase cellulose in stem cell walls. Results: Alfalfa plants were transformed with a truncated alfalfa phosphoenolpyruvate carboxylase gene promoter (PEPC7-P4) fused to an alfalfa nodule-enhanced SUS cDNA (MsSUS1) or the beta-glucuronidase (GUS) gene. Strong GUS expression was detected in xylem and phloem indicating that the PEPC7-P4 promoter was active in stem vascular tissue. In contrast to expectations, MsSUS1 transcript accumulation was reduced 75-90 % in alfalfa plants containing the PEPC7-P4::MsSUS1 transgene compared to controls. Enzyme assays indicated that SUS activity in stems of selected down-regulated transformants was reduced by greater than 95 % compared to the controls. Although SUS activity was detected in xylem and phloem of control plants by in situ enzyme assays, plants with the PEPC7-P4::MsSUS1 transgene lacked detectable SUS activity in post-elongation stem (PES) internodes and had very low SUS activity in elongating stem (ES) internodes. Loss of SUS protein in PES internodes of down-regulated lines was confirmed by immunoblots. Down-regulation of SUS expression and activity in stem tissue resulted in no obvious phenotype or significant change in cell wall sugar composition. However, alkaline/neutral (A/N) invertase activity increased in SUS down-regulated lines and high levels of acid invertase activity were observed. In situ enzyme assays of stem tissue showed localization of neutral invertase in vascular tissues of ES and PES internodes. Conclusions: These results suggest that invertases play a primary role in providing glucose for cellulose biosynthesis or compensate for the loss of SUS1 activity in stem vascular tissue. C1 [Samac, Deborah A.; Bucciarelli, Bruna; Yang, S. Samuel; O'Rourke, Jamie A.; Vance, Carroll P.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. [Samac, Deborah A.; Miller, Susan S.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Shin, Sanghyun; Vance, Carroll P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. RP Samac, DA (reprint author), ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. EM debby.samac@ars.usda.gov FU USDA-ARS CRIS [2640-12210-002-00D] FX We are grateful to Prem Chourey, USDA-ARS, University of Florida, for providing the maize sucrose synthase (SS2) antibody. The authors would also like to thank Ted Jeo, USDA-ARS-Plant Science Research Unit, St. Paul, MN, for performing the cell wall analysis, Melinda Dornbusch for production of transgenic plants, and Jon Mack for plant maintenance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Funding for this research was provided by USDA-ARS CRIS Project 2640-12210-002-00D. NR 54 TC 0 Z9 0 U1 6 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD DEC 1 PY 2015 VL 15 AR 283 DI 10.1186/s12870-015-0649-4 PG 13 WC Plant Sciences SC Plant Sciences GA CX4GA UT WOS:000365655900001 PM 26627884 ER PT J AU Yuan, X Cao, J Liu, T Li, YP Scannapieco, F He, X Oursler, MJ Zhang, X Vacher, J Li, C Olson, D Yang, S AF Yuan, X. Cao, J. Liu, T. Li, Y-P Scannapieco, F. He, X. Oursler, M. J. Zhang, X. Vacher, J. Li, C. Olson, D. Yang, S. TI Regulators of G protein signaling 12 promotes osteoclastogenesis in bone remodeling and pathological bone loss SO CELL DEATH AND DIFFERENTIATION LA English DT Article ID TERMINAL DIFFERENTIATION; BINDING-SPECIFICITY; MOLECULAR-CLONING; NUCLEAR-FACTOR; RGS PROTEINS; MICE DISPLAY; NULL MICE; IN-VITRO; RECEPTOR; ACTIVATION AB Regulators of G protein signaling (Rgs) have pivotal roles in controlling various cellular processes, such as cell differentiation. How Rgs proteins regulate osteoclast (OC) differentiation, function and bone homeostasis is poorly understood. It was previously demonstrated that Rgs12, the largest protein in the Rgs family, is predominantly expressed in OCs and regulates OC differentiation in vitro. To further understand the role and mechanism of Rgs12 in OC differentiation and bone diseases in vivo, we created OC-targeted Rgs12 knockout mice by using inducible Mx1-Cre and CD11b-Cre. Deletion of Rgs12 in hematopoietic cells or specifically in OC precursors resulted in increased bone mass with decreased OC numbers. Loss of Rgs12 impaired OC differentiation and function with impaired Ca2+ oscillations and reduced nuclear factor of activated T cells (NFAT) 2 expression. The introduction of wild-type osteoblasts did not rescue the defective osteoclastogenesis. Ectopic expression of NFAT2 rescued defective OC differentiation in CD11b; Rgs12(fl/fl) cells and promoted normal OC differentiation. Moreover, deletion of Rgs12 significantly inhibited pathological osteoclastogenesis and bone destruction in Rgs12-deficient mice that were subjected to ovariectomy and lipodysaccharide for bone loss. Thus our findings demonstrate that Rgs12 is an important regulator in OC differentiation and function and identify Rgs12 as a potential therapeutic target for osteoporosis and inflammation-induced bone loss. C1 [Yuan, X.; Liu, T.; Scannapieco, F.; Li, C.; Olson, D.; Yang, S.] SUNY Buffalo, Dept Oral Biol, Sch Dent Med, Buffalo, NY 14214 USA. [Cao, J.] USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Li, Y-P] Univ Alabama Birmingham, Dept Pathol, Birmingham, AL 35294 USA. [Scannapieco, F.; Yang, S.] SUNY Buffalo, Dev Genom Grp, New York State Ctr Excellence Bioinformat & Life, Buffalo, NY 14203 USA. [He, X.] China Med Univ, Affiliated Hosp 4, Dept Stomatol, Shenyang 110032, Liaoning, Peoples R China. [Oursler, M. J.] Mayo Clin, Dept Med, Endocrine Res Unit, Rochester, MN 55905 USA. [Zhang, X.] Univ Rochester, Med Ctr, Sch Med & Dent, Dept Orthoped,Ctr Musculoskeletal Res, Rochester, NY 14642 USA. [Vacher, J.] Univ Montreal, Dept Med, Div Expt Med, Inst Rech Clin Montreal, Montreal, PQ H2W 1R7, Canada. RP Yang, S (reprint author), SUNY Buffalo, Dept Oral Biol, Sch Dent Med, Buffalo, NY 14214 USA. EM sy47@buffalo.edu FU National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR061052, AR066101, AG048388]; National Institute of Aging [AR061052, AR066101, AG048388]; USDA Agricultural Research Service program 'Bone Metabolism in Obesity,' Current Research Information System [5450-51000-046-00D] FX We thank Dr Kristina Wasson-Blader and Miss Stacy Scheuneman for editing the manuscript, Dr Wade J Sigurdson, the director of the Flow Cytometry Facility at the School of Medicine and Biomedical Sciences, University at Buffalo, for assistance with the fluorescence microscope and Dr Peter J Bush for the technical assistance in using scanning electron microscope. This work was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases and National Institute of Aging under Award Numbers AR061052, AR066101 and AG048388 (to SY) and the USDA Agricultural Research Service program 'Bone Metabolism in Obesity,' Current Research Information System no. 5450-51000-046-00D to JC. NR 61 TC 6 Z9 6 U1 1 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1350-9047 EI 1476-5403 J9 CELL DEATH DIFFER JI Cell Death Differ. PD DEC PY 2015 VL 22 IS 12 BP 2046 EP 2057 DI 10.1038/cdd.2015.45 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA CW2UU UT WOS:000364849400014 PM 25909889 ER PT J AU Bair, EH Simenhois, R van Hervvijnen, A Birkeland, KW AF Bair, Edward H. Simenhois, Ron van Hervvijnen, Alec Birkeland, Karl W. TI Using 2 m Extended Column Tests to assess slope stability SO COLD REGIONS SCIENCE AND TECHNOLOGY LA English DT Article DE Snow; Avalanche; Stability tests; Extended Column Tests ID PROPAGATION; FRACTURE AB Extended Column Tests (ECTs) are used to assess crack initiation and propagation. Previous research shows that tests 90 cm in length may propagate, suggesting instability, while tests 2 m in length may not propagate, suggesting stability, for identical snowpacks. A practical question is: are 90 cm ECTs optimal for assessing stability? To test the added value of 2 m ECTs for stability evaluation, we collected data on 220 ECTs, with 136 side-by-side standard length ECTPs (full propagation indicating instability) followed by 2 m ECTs. We only performed 2 m ECTs after a standard ECT propagated because we assumed 2 m ECTs would not propagate if standard length tests did not. These tests were preceded by an a priori stability assessment. Our results show imbalances for both tests. The ECT had a similar probability of detection (0.88-0.92, POD), i.e. the ability to detect unstable conditions, as in previous studies, but a much lower probability of null events (0.54-0.75, PON), i.e. the ability to detect stable conditions, with variation due to the binary classification of "Fair" stability as stable or unstable. Adding a 2 m test after an ECTP result lowered the POD (0.49-0.58), but substantially raised the PON (0.88-0.98) of the combined tests. The proportion of tests in agreement, i.e. ECTP and 2 m ECTP, increases with decreasing stability. We conclude that an ECTP followed by a 2 m ECTP is a clear red flag, indicating instability. The interpretation of an ECTP followed by a 2 m ECTN/X (no propagation) is not clear. Though this result suggests stability, a high potential for a false stable result means we cannot recommend the 2 m ECT for binary stability assessments. (C) 2015 Elsevier B.V. All rights reserved. C1 [Bair, Edward H.] Univ Calif Santa Barbara, Earth Res Inst, Santa Barbara, CA 93106 USA. [Bair, Edward H.] US Army Corps, Engineers Cold Reg Res & Engn Lab, Hanover, NH USA. [Simenhois, Ron] Coeur, Juneau, AK USA. [van Hervvijnen, Alec] WSL Inst Snow & Avalanche Res SLF, Davos, Switzerland. [Birkeland, Karl W.] US Forest Serv, Natl Avalanche Ctr, Bozeman, MT USA. RP Bair, EH (reprint author), Univ Calif Santa Barbara, 6832 Ellison Hall, Santa Barbara, CA 93106 USA. EM nbair@eri.ucsb.edu RI van Herwijnen, Alec/D-5768-2015 FU US Army Corps of Engineers Cold Regions Research and Engineering fellowship FX We thank two anonymous reviewers for their helpful comments. We thank Brandon Schwartz and Andy Anderson at the Sierra Avalanche Center, Sue Burak at the Eastern Sierra Avalanche Center, and Mike Janes at Alaska Electric Light and Power for their stability tests. The first author was partly supported by a US Army Corps of Engineers Cold Regions Research and Engineering fellowship administered by ORISE/ORAU. NR 20 TC 1 Z9 1 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-232X EI 1872-7441 J9 COLD REG SCI TECHNOL JI Cold Reg. Sci. Tech. PD DEC PY 2015 VL 120 SI SI BP 191 EP 196 DI 10.1016/j.coldregions.2015.06.021 PG 6 WC Engineering, Environmental; Engineering, Civil; Geosciences, Multidisciplinary SC Engineering; Geology GA CW5RX UT WOS:000365055400020 ER PT J AU Deems, JS Gadomski, PJ Vellone, D Evanczyk, R LeWinter, AL Birkeland, KW Finnegan, DC AF Deems, Jeffrey S. Gadomski, Peter J. Vellone, Dominic Evanczyk, Ryan LeWinter, Adam L. Birkeland, Karl W. Finnegan, David C. TI Mapping starting zone snow depth with a ground-based lidar to assist avalanche control and forecasting SO COLD REGIONS SCIENCE AND TECHNOLOGY LA English DT Article DE Spatial variability; Snow depth; Lidar; Avalanche; Avalanche control; Laser scanning ID SPATIAL VARIABILITY; SLOPES AB The distribution of snow depth in avalanche starting zones exerts a strong influence on avalanche potential and character. Extreme depth changes over short distances are common, especially in wind-affected, above-treeline environments. Snow depth also affects the ease of avalanche triggering. Experience shows that avalanche reduction efforts are often more successful when targeting shallow trigger point areas near deeper slabs with explosives or ski cutting. Our paper explores the use of high-resolution (cm scale) snow depth and snow depth change maps from terrestrial laser scanning (TLS) data to quantify loading patterns for use in both pre-control planning and in post-control assessment. We present results from a pilot study in three study areas at the Arapahoe Basin ski area in Colorado, USA. A snow-free reference data set was collected in a summer TLS survey. Mapping multiple times during the snow season allowed us to produce time series maps of snow depth and snow depth change at high resolution to explore depth and slab thickness variations due to wind redistribution. We conducted surveys before and after loading events and control work, allowing the exploration of loading patterns, slab thickness, shot and ski cut locations, bed surfaces, entrainment, and avalanche characteristics. We also evaluate the state of TLS for use in operational avalanche control settings. (C) 2015 Elsevier B.V. All rights reserved. C1 [Deems, Jeffrey S.] Univ Colorado, Natl Snow & Ice Data Ctr, UCB 449, Boulder, CO 80309 USA. [Gadomski, Peter J.; LeWinter, Adam L.; Finnegan, David C.] US Army Corps, Engineers Cold Reg Res & Engn Lab, Hanover, NH 03755 USA. [Vellone, Dominic; Evanczyk, Ryan] Arapahoe Basin Ski Area, Dillon, CO 80435 USA. [Birkeland, Karl W.] US Forest Serv, USDA, Natl Avalanche Ctr, Bozeman, MT 59771 USA. RP Deems, JS (reprint author), Univ Colorado, Natl Snow & Ice Data Ctr, UCB 449, Boulder, CO 80309 USA. EM deems@nsidc.org RI Deems, Jeffrey/E-6484-2016 OI Deems, Jeffrey/0000-0002-3265-8670 FU CIRES Innovative Research Grant; Theo Meiners Research Grant from the American Avalanche Association FX We would like to acknowledge the Arapahoe Basin ski patrol, mountain operations, and management for enabling the data collection and collaborating towards operational integration. We are also grateful to Riegl Laser Measurement Systems GmbH for their innovation and leadership - their new long-range TLS systems make this project possible. Thanks to our two reviewers, whose comments and suggestions greatly improved this manuscript. This work was partially supported by a CIRES Innovative Research Grant, and by a Theo Meiners Research Grant from the American Avalanche Association - we are indebted to Theo for his energy and inspiration to incorporate new science and tools into operational avalanche decision-making. NR 25 TC 5 Z9 5 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-232X EI 1872-7441 J9 COLD REG SCI TECHNOL JI Cold Reg. Sci. Tech. PD DEC PY 2015 VL 120 SI SI BP 197 EP 204 DI 10.1016/j.coldregions.2015.09.002 PG 8 WC Engineering, Environmental; Engineering, Civil; Geosciences, Multidisciplinary SC Engineering; Geology GA CW5RX UT WOS:000365055400021 ER PT J AU Marienthal, A Hendrikx, J Birkeland, K Irvine, KM AF Marienthal, Alex Hendrikx, Jordy Birkeland, Karl Irvine, Kathryn M. TI Meteorological variables to aid forecasting deep slab avalanches on persistent weak layers SO COLD REGIONS SCIENCE AND TECHNOLOGY LA English DT Article DE Deep slabs; Avalanche forecasting; Classification trees; Random forest; Persistent weak layers ID CLASSIFICATION TREES; SNOW; PREDICTION; STABILITY; MOUNTAIN; FRACTURE AB Deep slab avalanches are particularly challenging to forecast. These avalanches are difficult to trigger, yet when they release they tend to propagate far and can result in large and destructive avalanches. We utilized a 44-year record of avalanche control and meteorological data from Bridger Bowl ski area in southwest Montana to test the usefulness of meteorological variables for predicting seasons and days with deep slab avalanches. We defined deep slab avalanches as those that failed on persistent weak layers deeper than 0.9 m, and that occurred after February 1st. Previous studies often used meteorological variables from days prior to avalanches, but we also considered meteorological variables over the early months of the season. We used classification trees and random forests for our analyses. Our results showed seasons with either dry or wet deep slabs on persistent weak layers typically had less precipitation from November through January than seasons without deep slabs on persistent weak layers. Days with deep slab avalanches on persistent weak layers often had warmer minimum 24-hour air temperatures, and more precipitation over the prior seven days, than days without deep slabs on persistent weak layers. Days with deep wet slab avalanches on persistent weak layers were typically preceded by three days of above freezing air temperatures. Seasonal and daily meteorological variables were found useful to aid forecasting dry and wet deep slab avalanches on persistent weak layers, and should be used in combination with continuous observation of the snowpack and avalanche activity. (C) 2015 Elsevier B.V. All rights reserved. C1 [Marienthal, Alex; Hendrikx, Jordy; Birkeland, Karl] Montana State Univ, Snow & Avalanche Lab, Bozeman, MT 59717 USA. [Birkeland, Karl] US Forest Serv, USDA, Natl Avalanche Ctr, Bozeman, MT USA. [Irvine, Kathryn M.] US Geol Survey, Northern Rocky Mt Sci Ctr, Reston, VA USA. RP Marienthal, A (reprint author), POB 6337, Bozeman, MT 59771 USA. EM alexmarienthal1@gmail.com OI Hendrikx, Jordy/0000-0001-6194-3596 FU American Avalanche Association Graduate Student Research Grant; Montana State University; Bridger Bowl; Gallatin National Forest Avalanche Center; Snow and Avalanche Laboratory at Montana State University FX The authors would like to acknowledge the American Avalanche Association Graduate Student Research Grant, Montana State University, and Bridger Bowl for financial support of this research. We also thank the Bridger Bowl ski patrol for collection and upkeep of weather and avalanche control records. Additional input and support that was crucial to this research came from Doug Richmond and Randy Elliot of Bridger Bowl, Scotty Savage and the Big Sky ski patrol, the Gallatin National Forest Avalanche Center, and the Snow and Avalanche Laboratory at Montana State University. We also thank Mark Greenwood and two anonymous reviewers for reviewing this manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government NR 44 TC 2 Z9 2 U1 3 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-232X EI 1872-7441 J9 COLD REG SCI TECHNOL JI Cold Reg. Sci. Tech. PD DEC PY 2015 VL 120 SI SI BP 227 EP 236 DI 10.1016/j.coldregions.2015.08.007 PG 10 WC Engineering, Environmental; Engineering, Civil; Geosciences, Multidisciplinary SC Engineering; Geology GA CW5RX UT WOS:000365055400024 ER PT J AU Horak, KE Volker, SF Campton, CM AF Horak, Katherine E. Volker, Steven F. Campton, Christopher M. TI Increased diphacinone and chlorophacinone metabolism in previously exposed wild caught voles, Microtus californicus SO CROP PROTECTION LA English DT Article DE Anticoagulant resistance; Voles; Castroville; CA; Metabolism; Chlorophacinone; Diphacinone ID SUSCEPTIBLE NORWAY RATS; RATTUS-NORVEGICUS; WARFARIN RESISTANCE; BROWN-RAT; EXPRESSION; CYTOCHROME-P450; ANTICOAGULANTS; RODENTICIDES; GENES AB Rodents have been noteworthy pests in agricultural areas for decades. Because rodents impact diverse ecosystems, anticoagulant rodenticides have been heavily used throughout the world to control rodent populations. This continued use has led to the development of resistance to anticoagulant rodenticides in some populations of targeted rodents. Although many studies have investigated the genetic and molecular basis of anticoagulant resistance, few have focused on potential changes in metabolic function of resistant animals. In this study, vole (Microtus californicus, Peale) liver microsome preparations were made from unexposed animals living in areas that had never used anticoagulant rodenticides for either crop protection or for the control of commensal rodents and exposed voles living in artichoke fields that have used anticoagulant rodenticides since the mid-1990s. Using these microsome preparations, the metabolism of diphacinone and chlorophacinone was tested. Microsomes from both male and female voles from exposed areas metabolized significantly more anticoagulant than unexposed animals. Also, both exposed and unexposed animals metabolized more diphacinone than chlorophacinone. These findings suggest that alterations in metabolic function may play a role in anticoagulant resistance. Published by Elsevier Ltd. C1 [Horak, Katherine E.; Volker, Steven F.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Campton, Christopher M.] Natl Resources Conservat Serv, USDA, Wheatland, WY 82201 USA. RP Horak, KE (reprint author), APHIS Wildlife Serv, Natl Wildlife Res Ctr, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM katherine.e.horak@aphis.usda.gov FU Vertebrate Pest Control Research Advisory Committee of California FX The authors gratefully acknowledge Dale Huss and Ocean Mist Farms for allowing trapping activates on their agricultural lands. This research was supported by Vertebrate Pest Control Research Advisory Committee of California. NR 27 TC 2 Z9 2 U1 3 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD DEC PY 2015 VL 78 BP 35 EP 39 DI 10.1016/j.cropro.2015.08.011 PG 5 WC Agronomy SC Agriculture GA CW3IR UT WOS:000364886300006 ER PT J AU Hupert, N Biala, K Holland, T Baehr, A Hasan, A Harvey, M AF Hupert, Nathaniel Biala, Karen Holland, Tara Baehr, Avi Hasan, Aisha Harvey, Melissa TI Optimizing Health Care Coalitions: Conceptual Frameworks and a Research Agenda SO DISASTER MEDICINE AND PUBLIC HEALTH PREPAREDNESS LA English DT Article DE disaster planning; health care coalitions; emergency preparedness; delivery of health care ID HIGH-RELIABILITY ORGANIZATIONS; PREPAREDNESS; CHALLENGES AB The US health care system has maintained an objective of preparedness for natural or manmade catastrophic events as part of its larger charge to deliver health services for the American population. In 2002, support for hospital-based preparedness activities was bolstered by the creation of the National Bioterrorism Hospital Preparedness Program, now called the Hospital Preparedness Program, in the US Department of Health and Human Services. Since 2012, this program has promoted linking health care facilities into health care coalitions that build key preparedness and emergency response capabilities. Recognizing that well-functioning health care coalitions can have a positive impact on the health outcomes of the populations they serve, this article informs efforts to optimize health care coalition activity. We first review the landscape of health care coalitions in the United States. Then, using principles from supply chain management and high-reliability organization theory, we present 2 frameworks extending beyond the Office of the Assistant Secretary for Preparedness and Response's current guidance in a way that may help health care coalition leaders gain conceptual insight into how different enterprises achieve similar ends relevant to emergency response. We conclude with a proposed research agenda to advance understanding of how coalitions can contribute to the day-to-day functioning of health care systems and disaster preparedness. (Disaster Med Public Health Preparedness. 2015;9:717-723) C1 [Hupert, Nathaniel; Biala, Karen; Hasan, Aisha; Harvey, Melissa] US Dept HHS, Off Assistant Secretary Preparedness & Response, Washington, DC 20201 USA. [Hupert, Nathaniel] Weill Cornell Med Coll, Dept Healthcare Policy & Res, New York, NY USA. [Biala, Karen] Food & Nutr Serv, USDA, Alexandria, VA USA. [Holland, Tara] Gap Solut Inc, US Dept HHS, Off Assistant Secretary Preparedness & Response, Washington, DC USA. [Baehr, Avi] US Dept HHS, Off Dis Prevent & Hlth Promot, Washington, DC 20201 USA. [Baehr, Avi] Univ Penn, Perelman Sch Med, Philadelphia, PA 19104 USA. RP Holland, T (reprint author), 200 Independence Ave SW, Washington, DC 20201 USA. EM tara.holland@hhs.gov NR 24 TC 0 Z9 0 U1 1 U2 3 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1935-7893 EI 1938-744X J9 DISASTER MED PUBLIC JI Dis. Med. Public Health Prep. PD DEC PY 2015 VL 9 IS 6 BP 717 EP 723 DI 10.1017/dmp.2015.144 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CW9MP UT WOS:000365323100020 PM 26545194 ER PT J AU Caipo, M Cahill, S Kenny, M Wachsmuth, K Toyofuku, H Hielm, S Carolissen, V Bruno, A Mulholland, C Kojima, M Esteban, E AF Caipo, M. Cahill, S. Kenny, M. Wachsmuth, K. Toyofuku, H. Hielm, S. Carolissen, V. Bruno, A. Mulholland, C. Kojima, M. Esteban, E. TI The development of illustrative examples for the establishment and application of microbiological criteria for foods and their role in international standard development SO FOOD CONTROL LA English DT Editorial Material C1 [Caipo, M.] Reg Off Latin Amer & Caribbean, Food & Agr Org United Nat, Santiago, Chile. [Cahill, S.; Kenny, M.] Food & Agr Org United Nat, Milan, Italy. [Toyofuku, H.] Yamaguchi Univ, Joint Fac Vet Med, Yamaguchi, Japan. [Hielm, S.; Carolissen, V.; Bruno, A.] Food & Agr Org United Nat, Joint FAO WHO Food Stand Programme, Milan, Italy. [Mulholland, C.] WHO, Geneva, Switzerland. [Kojima, M.] Minist Hlth, Dept Food Safety, Labour & Welf, Tokyo, Japan. [Esteban, E.] USDA, FSIS OPHS, New York, NY USA. RP Caipo, M (reprint author), Reg Off Latin Amer & Caribbean, Food & Agr Org United Nat, Santiago, Chile. EM Marisa.Caipo@fao.org NR 8 TC 1 Z9 1 U1 0 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD DEC PY 2015 VL 58 SI SI BP 1 EP 6 DI 10.1016/j.foodcont.2015.06.043 PG 6 WC Food Science & Technology SC Food Science & Technology GA CW5TI UT WOS:000365059100001 ER PT J AU Scott, VN Powell, M Cabrera, J Carullo, ME Martinez, I Lohachoompol, V AF Scott, Virginia N. Powell, Mark Cabrera, Josefina Carullo, Maria E. Martinez, Ines Lohachoompol, Virachnee TI Development of microbiological criteria to assess the acceptability of a food lot - An example for milk powder SO FOOD CONTROL LA English DT Article DE Microbiological criterion; Sampling plans; Lot acceptability; Verification; Milk powder ID PERFORMANCE AB Milk powder to be consumed without further treatment to inactivate microorganisms was selected to illustrate the process for establishing and applying a microbiological criterion to assess the acceptability of a food lot. Example criteria (size of analytical unit, sampling plan and limits) were specified for mesophilic aerobic colony count and Enterobacteriaceae as indicators of the adequacy of Good Hygienic Practices and for Salmonella as a food safety criterion. Performance characteristics were determined for each criterion using four values for standard deviation of the microbial counts to illustrate how sampling plan performance depends on the within-lot standard deviation, which is uncertain for any given lot and varies among lots. Methods of analysis were specified. A description of how to interpret the results and examples of actions that could be taken by food business operators and competent authorities are provided. (C) 2014 Published by Elsevier Ltd. C1 [Scott, Virginia N.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. [Powell, Mark] USDA, Off Risk Assessment & Cost Benefit Anal, Washington, DC 20250 USA. [Cabrera, Josefina] Minist Hlth, Natl Food Inst, INAL ANMAT, Microbiol, Buenos Aires, DF, Argentina. [Carullo, Maria E.] Agri Food SENASA, Natl Serv Hlth & Qual, RA-1063 Caba, Argentina. [Martinez, Ines] Technol Lab Uruguay Latu, Montevideo 11500, Uruguay. [Lohachoompol, Virachnee] Minist Agr & Cooperat, Natl Bur Agr Commod & Food Stand, Bangkok 10900, Thailand. RP Scott, VN (reprint author), US FDA, Ctr Food Safety & Appl Nutr, 5100 Paint Branch Pkwy,HFS-300, College Pk, MD 20740 USA. EM jennyscott@verizon.net NR 12 TC 2 Z9 2 U1 1 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD DEC PY 2015 VL 58 SI SI BP 12 EP 16 DI 10.1016/j.foodcont.2014.09.026 PG 5 WC Food Science & Technology SC Food Science & Technology GA CW5TI UT WOS:000365059100003 ER PT J AU Powell, MR AF Powell, Mark R. TI The food safety impact of the Codex sampling plans for Listeria monocytogenes in ready-to-eat foods: An empirical case study applying the FAO/WHO sampling plan tool SO FOOD CONTROL LA English DT Article DE Sampling plan; Microbiological criteria; Listeria monocytogenes; Codex Alimentarius ID INSPECTION POLICY; ISO-METHOD-11290; VALIDATION; PENALTIES; HAZARD AB Sampling plans are specified by the Codex Alimentarius Commission microbiological criteria for Listeria monocytogenes in ready-to-eat foods. This case study evaluates the direct food safety impact of the Codex sampling plans as estimated by the FAO/WHO web-based microbiological sampling plan analysis tool under different assumptions about the pathogen distribution, test procedures, and the fraction of lots tested. The case study uses L. monocytogenes concentration data available for deli-type salads to empirically illustrate application of the sampling tool. The results indicate that the estimated impact of the sampling plans is dependent on the partitioning of total observed variance into its within- and between-lot components. The presence-absence based sampling plan is relatively insensitive to the substantial uncertainty and variability of the sensitivity of the reference method for detection of L. monocytogenes. The analytical sample size for enumeration impacts the ability of the concentration-based sampling plan to discriminate between compliant and non-compliant lots. Reducing the frequency of lot testing dramatically changes the statistical properties of the sampling schemes. Skip-lot sampling places greater importance on compliance assurance than on the direct, curative impact of lot acceptance sampling. Published by Elsevier Ltd. C1 [Powell, Mark R.] USDA, Off Risk Assessment & Cost Benefit Anal, Washington, DC 20250 USA. RP Powell, MR (reprint author), USDA, Off Risk Assessment & Cost Benefit Anal, 1400 Independence Ave SW,Rm 4032, Washington, DC 20250 USA. EM mpowell@oce.usda.gov NR 30 TC 1 Z9 1 U1 3 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD DEC PY 2015 VL 58 SI SI BP 43 EP 50 DI 10.1016/j.foodcont.2014.04.033 PG 8 WC Food Science & Technology SC Food Science & Technology GA CW5TI UT WOS:000365059100008 ER PT J AU Martinez, OJA Ackerman, EJM Montiel, DG Parrotta, JA AF Martinez, Oscar J. Abelleira Ackerman, Elvia J. Melendez Montiel, Diana Garcia Parrotta, John A. TI Seed dispersal turns an experimental plantation on degraded land into a novel forest in urban northern Puerto Rico SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Albizia lebbek; Animal seed dispersal; Introduced N-fixing tree monocultures; Leucaena leucocephala; Native species restoration; Tropical coastal pasture reforestation ID SUBTROPICAL DRY FOREST; TROPICAL RAIN-FOREST; LEUCAENA-LEUCOCEPHALA; ABANDONED PASTURE; REGENERATION; SUCCESSION; BIODIVERSITY; ECOSYSTEMS; RESTORATION; LANDSCAPE AB Planting tree species with desirable traits may catalyze forest regeneration in increasingly common degraded lands by restoring soil properties and attracting seed dispersers. We sampled forest regeneration in an experimental plantation of Albizia lebbek, an introduced N-fixing species, on a degraded pasture in northern Puerto Rico, 27 years after its establishment. We hypothesized that the abundance of animal-dispersed tree species within this unmanaged plantation would be (1) greater than in adjacent pastures; (2) higher at distances closer to secondary forest; and (3) increased over the 20 years since floristic surveys were last conducted in the site. We sampled forest structure and tree species composition, and compared the abundance of animal-dispersed tree species on plots located within the adjacent pasture and the A. lebbek plantation, within the plantation in plots distanced at 10, 35, and 60 m from secondary forest, and within the plantation at 7 vs. 27 years after plantation establishment. We found higher abundance of animal-dispersed tree species in the A. lebbek plantation than in the pasture. Distance from secondary forest had no effect on the abundance of animal-dispersed species. The abundance of animal-dispersed species increased by 14% between 7 and 27 years after plantation establishment. In all, these results indicate that A. lebbek trees facilitated the establishment of native animal-dispersed species by shading out tall grasses and acting as perches for animals throughout the plantation. We found that A. lebbek lost its dominance in the site to another introduced N-fixing species, Leucaena leucocephala, possibly due to unfavorable traits such as susceptibility to pathogens. Our findings document a transition from an experimental plantation to a novel urban forest that harbors an array of native tree species, largely due to the digiersal of seeds by animals. A noteworthy example is the presence of Guaiacum officinale, an animal-dispersed and endangered tree species native to the dry forests of southern Puerto Rico. Our results highlight how monocultures of introduced tree species with desirable traits can catalyze the regeneration of native tree species in chronically degraded lands. Published by Elsevier B.V. C1 [Martinez, Oscar J. Abelleira] US Forest Serv, Int Inst Trop Forestry, USDA, San Juan, PR 00926 USA. [Martinez, Oscar J. Abelleira] Univ Puerto Rico, Coll Agr Sci, Dept Agroenvironm Sci, Mayaguez, PR 00681 USA. [Ackerman, Elvia J. Melendez; Montiel, Diana Garcia] Univ Puerto Rico, Coll Nat Sci, Dept Environm Sci, San Juan, PR 00936 USA. [Parrotta, John A.] US Forest Serv, USDA, Res & Dev, Washington, DC 20024 USA. RP Martinez, OJA (reprint author), Univ Puerto Rico, Coll Agr Sci, Dept Agroenvironm Sci, Call Box 9000, Mayaguez, PR 00681 USA. EM ojabelleira@gmail.com NR 52 TC 0 Z9 0 U1 3 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 1 PY 2015 VL 357 BP 68 EP 75 DI 10.1016/j.foreco.2015.08.008 PG 8 WC Forestry SC Forestry GA CW3KY UT WOS:000364892400008 ER PT J AU Brockway, DG Outcalt, KW AF Brockway, Dale G. Outcalt, Kenneth W. TI Influence of selection systems and shelterwood methods on understory plant communities of longleaf pine forests in flatwoods and uplands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pinus palustris Mill.; Foliar cover; Species diversity; Even-aged silviculture; Uneven-aged silviculture; Pro-B method ID SOUTHEASTERN UNITED-STATES; PRESCRIBED FIRE; ECOSYSTEMS; PALUSTRIS; RESTORATION; FLORIDA; SILVICULTURE; MANAGEMENT; VEGETATION; REDUCTION AB Although longleaf pine (Pinus palustris Mill.) forests have mostly been managed with even-aged methods, interest has been rising in uneven-aged systems, as a means of achieving a broader range of stewardship objectives. Selection silviculture has been practiced on a limited scale in longleaf pine, but difficulty of using traditional approaches and absence of an evaluation across a range of site types has left managers in doubt about its suitability. This study was conducted to quantify the effects on understory plant communities of applying single-tree selection, group selection, irregular shelterwood and uniform shelterwood in longleaf pine forests on flatwoods and uplands of the southeastern United States. Wiregrass (Aristida beyrichiana Trin. & Rupr.) and numerous other graminoids are highly desirable understory species, because they facilitate the essential ecological process of recurrent surface fire that sustains longleaf pine ecosystems. Forbs such as partridge pea (Chamaecrista fasciculata (Michx.) Greene) and low shrubs such as gopherapple (Licania michauxii Prance), blueberries (Vaccinium spp. L) and huckleberries (Gaylussacia spp. Kunth) are also desirable as components of good wildlife habitat. Selection treatments reduced stand basal area to similar to 11.5 m(2) ha(-1) and shelterwood treatments left a basal area of similar to 5.8 m(2) ha(-1). While higher levels of logging traffic from shelterwood treatment caused a significant decline in sawpalmetto (Serenoa repens W. Bartram) cover and increases in wiregrass at the flatwoods site, on the upland site it resulted in a sharp decline in wiregrass and silverthread goldaster (Pityopsis graminifolia (Michx.) Nutt.). Absence of prescribed fire during the post-treatment years led to progressive increases for shrub cover broadly across the flatwoods. Group selection caused modest understory change in flatwoods (temporary decrease in shrubs and increase in wiregrass), but resulted in a doubling of understory plant cover on uplands, with significant increases for hardwood tree seedlings, shrubs, vines, wiregrass, forbs and ferns. Single-tree selection caused no lasting impact on saw-palmetto, a decline in gallberry (Ilex glabra (L.) A. Gray) and increase in wiregrass in flatwoods and was related on uplands to increases in oak (Quercus spp. L.), dangleberry (Gaylusaccia frondosa (L.) Torr & A. Gray ex. Torr.), broomsedge blue-stem (Andropogon virginicus L.) and several forbs. Single-tree selection produced less change in the forest than group selection, which caused less alteration than shelterwood treatment. Selection silviculture appears to be a lower risk option for guiding longleaf pine forests along a trajectory of gradual improvement, with adjustments provided by frequent surface fires and periodic tree harvest. Long-term observation is needed to verify that selection can sustain diverse plant communities on sites characterized by differing environments. Published by Elsevier B.V. C1 [Brockway, Dale G.] US Forest Serv, Southern Res Stn, USDA, Auburn, AL 36849 USA. [Outcalt, Kenneth W.] US Forest Serv, Southern Res Stn, USDA, Athens, GA 30602 USA. RP Brockway, DG (reprint author), US Forest Serv, Southern Res Stn, USDA, 521 Devall Dr, Auburn, AL 36849 USA. EM dbrockway@fs.fed.us FU State of Florida, Division of Forestry [SRS-04-CO-11330123-043] FX We thank David Dyson, Matt Reilly, David Combs, Jeremy Waites, David Jones, Bryan Bolger, Ron Tucker, Chris Colburn, Andy Lamborn, Eric Neiswanger, Jason O'Shell, Mike Allen and Tom Phillips for field assistance and two anonymous reviewers for comments which improved this manuscript. Funding for this study was provided by the State of Florida, Division of Forestry [agreement SRS-04-CO-11330123-043]. NR 52 TC 0 Z9 0 U1 10 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 1 PY 2015 VL 357 BP 138 EP 150 DI 10.1016/j.foreco.2015.08.020 PG 13 WC Forestry SC Forestry GA CW3KY UT WOS:000364892400015 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI Increased CaCl2, MgSO4, and KH2PO4 improve the growth of micropropagated red raspberries SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Calcium; Growth medium; Magnesium; Phosphorus; Rubus ID TOBACCO TISSUE CULTURES; MINERAL-NUTRITION; IN-VITRO; MULTIPLICATION; INVITRO; SHOOT AB The genetic variation present in red raspberries makes it difficult to successfully apply a standard in vitro growth medium. An initial study modeling Murashige and Skoog (MS) medium minerals concluded that the mesos (CaCl2, MgSO4, and KH2PO4) components significantly affected red raspberry (Rubus idaeus L.) shoot quality and growth. To determine the effects of the individual mesos components, a three-dimensional design based on response surface methodology was employed. Shoot cultures of five cultivars were evaluated for quality, multiplication, shoot length, leaf characteristics, and mineral content. The resulting model indicated that improved growth and quality of all cultivars required significantly higher (p a parts per thousand currency signaEuro parts per thousand 0.05) concentrations of some or all of the three mesos compared to MS medium. Higher concentrations of CaCl2 and KH2PO4 significantly increased shoot length for most cultivars. MgSO4 had the greatest effect on leaf characteristics. Shoot mineral content was directly related to the mineral content of the medium. Although individual cultivars varied in optimal amounts of each component, high-quality shoots could be produced for all cultivars with 2.5-3.0x the MS concentrations of all three salts. C1 [Poothong, Sukalya] Univ Phayao, Sch Agr & Nat Resources, Muang Dist Phayao 56000, Thailand. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Reed, BM (reprint author), USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM sukalya_p@hotmail.com; reedba@oregonstate.edu FU US Department of Agriculture, Agricultural Research Service CRIS project [5358-21000-033D]; Royal Thai Government FX This project was funded by the US Department of Agriculture, Agricultural Research Service CRIS project 5358-21000-033D. Sukalya Poothong acknowledges the financial support of a Royal Thai Government Scholarship during her Ph.D. studies at Oregon State University. NR 30 TC 1 Z9 1 U1 3 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD DEC PY 2015 VL 51 IS 6 BP 648 EP 658 DI 10.1007/s11627-015-9720-y PG 11 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA CW8UG UT WOS:000365274200007 ER PT J AU Hughes, SR Cox, EJ Bang, SS Pinkelman, RJ Lopez-Nunez, JC Saha, BC Qureshi, N Gibbons, WR Fry, MR Moser, BR Bischoff, KM Liu, SQ Sterner, DE Butt, TR Riedmuller, SB Jones, MA Riano-Herrera, NM AF Hughes, Stephen R. Cox, Elby J. Bang, Sookie S. Pinkelman, Rebecca J. Carlos Lopez-Nunez, Juan Saha, Badal C. Qureshi, Nasib Gibbons, William R. Fry, Michelle R. Moser, Bryan R. Bischoff, Kenneth M. Liu, Siqing Sterner, David E. Butt, Tauseef R. Riedmuller, Steven B. Jones, Marjorie A. Riano-Herrera, Nestor M. TI Process for Assembly and Transformation into Saccharomyces cerevisiae of a Synthetic Yeast Artificial Chromosome Containing a Multigene Cassette to Express Enzymes That Enhance Xylose Utilization Designed for an Automated Platform SO JALA LA English DT Article DE synthetic yeast chromosome assembly; stable cellulosic yeast; yeast artificial expression chromosome; multigene expression cassette; synthetic biology platform ID ENZYMATIC SACCHARIFICATION; ETHANOL-PRODUCTION; CORN FIBER; FERMENTATION; STRAIN; GLUCOSE; INDUSTRIAL; ISOMERASE; ACID; PRETREATMENT AB A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed and transformed into Saccharomyces cerevisiae to demonstrate feasibility as a stable protein expression system in yeast and to design an assembly process suitable for an automated platform. Expression of XI and XKS from the YAC was confirmed by Western blot and PCR analyses. The recombinant and wild-type strains showed similar growth on plates containing hexose sugars, but only recombinant grew on D-xylose and L-arabinose plates. In glucose fermentation, doubling time (4.6 h) and ethanol yield (0.44 g ethanol/g glucose) of recombinant were comparable to wild type (4.9 h and 0.44 g/g). In whole-corn hydrolysate, ethanol yield (0.55 g ethanol/g [glucose + xylose]) and xylose utilization (38%) for recombinant were higher than for wild type (0.47 g/g and 12%). In hydrolysate from spent coffee grounds, yield was 0.46 g ethanol/g (glucose + xylose), and xylose utilization was 93% for recombinant. These results indicate introducing a YAC expressing XI and XKS enhanced xylose utilization without affecting integrity of the host strain, and the process provides a potential platform for automated synthesis of a YAC for expression of multiple optimized genes to improve yeast strains. C1 [Hughes, Stephen R.; Cox, Elby J.; Bischoff, Kenneth M.; Liu, Siqing] ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. [Cox, Elby J.; Fry, Michelle R.] Bradley Univ, Dept Chem & Biochem, Peoria, IL 61625 USA. [Bang, Sookie S.; Pinkelman, Rebecca J.] South Dakota Sch Mines & Technol, Chem & Biol Engn, Rapid City, SD USA. [Carlos Lopez-Nunez, Juan; Riano-Herrera, Nestor M.] Natl Federat Coffee Growers Colombia FNC, Natl Coffee Res Ctr Cenicafe, Manizales, Caldas, Colombia. [Saha, Badal C.; Qureshi, Nasib] ARS, USDA, NCAUR, Bioenergy Res Unit, Peoria, IL 61604 USA. [Gibbons, William R.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Moser, Bryan R.] ARS, USDA, NCAUR, Biooils Res Unit, Peoria, IL 61604 USA. [Sterner, David E.] Progenra Inc, Malvern, PA USA. [Butt, Tauseef R.] LifeSensors Inc, Malvern, PA USA. [Riedmuller, Steven B.] Hudson Control Grp Inc, Springfield, NJ USA. [Jones, Marjorie A.] Illinois State Univ, Dept Chem, Normal, IL 61761 USA. RP Hughes, SR (reprint author), ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. EM stephen.hughes@ars.usda.gov NR 41 TC 1 Z9 1 U1 5 U2 20 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 2211-0682 EI 1540-2452 J9 JALA-J LAB AUTOM JI JALA PD DEC PY 2015 VL 20 IS 6 BP 621 EP 635 DI 10.1177/2211068215573188 PG 15 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CX1AZ UT WOS:000365429900002 PM 25720598 ER PT J AU Bautista, E Warrick, AW AF Bautista, E. Warrick, A. W. TI Closure to "New Results for an Approximate Method for Calculating Two-Dimensional Furrow Infiltration" by E. Bautista, A. W. Warrick, and T. S. Strelkoff SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Editorial Material ID BROOKS-COREY C1 [Bautista, E.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Warrick, A. W.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. RP Bautista, E (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM Eduardo.Bautista@ars.usda.gov; aww@cals.arizona.edu NR 4 TC 0 Z9 0 U1 0 U2 0 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD DEC PY 2015 VL 141 IS 12 AR UNSP 07015028 DI 10.1061/(ASCE)IR.1943-4774.0000753 PG 2 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA CW6RV UT WOS:000365126100011 ER PT J AU Booker, JD Lascano, RJ Molling, CC Zartman, RE Acosta-Martinez, V AF Booker, J. D. Lascano, R. J. Molling, C. C. Zartman, R. E. Acosta-Martinez, V. TI Temporal and spatial simulation of production-scale irrigated cotton systems SO PRECISION AGRICULTURE LA English DT Article DE Precision agriculture; Spatial and temporal simulation; Center pivot irrigation; Cotton; Soil water content; Plant height ID TOPOGRAPHICALLY COMPLEX LANDSCAPES; SOUTHERN HIGH-PLAINS; TEXAS HIGH-PLAINS; SOIL-WATER STATUS; LINT YIELD; MANAGEMENT STRATEGIES; MODEL; PRECISION; ACCURACY; CONDUCTIVITY AB Site-specific management of cotton (Gossypium hirsutum) cropping systems at the production-scale requires information regarding environmental interactions across the landscape. Landscape-scale cotton models could track these interactions and be integrated into future decision support tools designed to manage variable inputs; however, modeling of cotton systems across the landscape has not been evaluated. Cotton production in the Southern Texas High Plains is dependent on irrigation from the Ogallala Aquifer, and thus tracking soil water content across fields could help producers plan their use of diminishing aquifer resources. Our hypothesis was that the PALMScot model, a grid-based landscape-scale cotton model, would capture spatial and temporal variability and environmental interactions affecting soil water and plant growth within a 70-ha field throughout two contrasting growing seasons, without adjustment of input parameters for the model. Thus, our objective was to compare values of soil water content and crop height calculated by the PALMScot model with corresponding field measured values at multiple locations across a fine textured, pivot irrigated production cotton field during two growing seasons. The PALMScot model calculated values of soil water and crop height across the field with a root mean squared deviation (RMSD) for soil water content in the 1.0-m profile a parts per thousand currency sign0.032 m(3)/m(3) and most Nash-Sutcliffe efficiency (NSE) values a parts per thousand yen0.48. Values of RMSD for crop height were a parts per thousand currency sign0.10 m at all locations in 2010 and 2011. We conclude that PALMScot correctly and efficiently calculated soil water content and crop height across the field, throughout each season, and the model has potential as a site-specific management tool for cotton cropping systems. C1 [Booker, J. D.; Zartman, R. E.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Lascano, R. J.; Acosta-Martinez, V.] USDA ARS, Cropping Syst Res Lab, Wind Eros & Water Conservat, Lubbock, TX 79415 USA. [Molling, C. C.] Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, Madison, WI 53706 USA. RP Lascano, RJ (reprint author), USDA ARS, Cropping Syst Res Lab, Wind Eros & Water Conservat, 3810 4th St, Lubbock, TX 79415 USA. EM robert.lascano@ars.usda.gov OI Lascano, Robert/0000-0003-1520-7154 FU Ogallala Aquifer Program FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 75 TC 0 Z9 0 U1 4 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD DEC PY 2015 VL 16 IS 6 BP 630 EP 653 DI 10.1007/s11119-015-9397-6 PG 24 WC Agriculture, Multidisciplinary SC Agriculture GA CW7ZM UT WOS:000365218100003 ER PT J AU Huang, Q Chen, YP Wang, RW Schwarz, RS Evans, JD AF Huang, Qiang Chen, Yanping Wang, Rui Wu Schwarz, Ryan S. Evans, Jay D. TI Honey bee microRNAs respond to infection by the microsporidian parasite Nosema ceranae SO SCIENTIFIC REPORTS LA English DT Article ID RNA INTERFERENCE PATHWAYS; APIS-MELLIFERA; DIFFERENTIAL EXPRESSION; PROTOZOAN PARASITES; GENES; DISTINCT; PACKAGE AB In order to study the effects of Nosema ceranae infection on honey bee microRNA (miRNA) expression, we deep-sequenced honey bee miRNAs daily across a full 6-day parasite reproduction cycle. Seventeen miRNAs were differentially expressed in honey bees infected by N. ceranae that potentially target over 400 genes predicted to primarily involve ion binding, signaling, the nucleus, transmembrane transport, and DNA binding. Based on Enzyme Code analysis, nine biological pathways were identified by screening target genes against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, seven of which involved metabolism. Our results suggest that differentially expressed miRNAs regulate metabolism related genes of host honey bees in response to N. ceranae infection. C1 [Huang, Qiang; Wang, Rui Wu] Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China. [Huang, Qiang; Chen, Yanping; Schwarz, Ryan S.; Evans, Jay D.] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. RP Huang, Q (reprint author), Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Peoples R China. EM qiang-huang@live.com; wangrw@mail.kiz.ac.cn; Jay.Evans@ARS.USDA.GOV OI Evans, Jay/0000-0002-0036-4651 FU USDA-NIFA [2014-67013-21784] FX We thank Mrs. Dawn Lopez and Michele Hamilton for technical support. We appreciate Mr. Jiajie Sun for statistical support. The work is supported by USDA-NIFA grant 2014-67013-21784. NR 29 TC 0 Z9 0 U1 5 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD DEC 1 PY 2015 VL 5 AR 17494 DI 10.1038/srep17494 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX2BF UT WOS:000365499200003 PM 26620304 ER PT J AU Han, YH Blechl, A Wang, DW AF Han, Yonghua Blechl, Ann Wang, Daowen TI The distribution of cotransformed transgenes in particle bombardment-mediated transformed wheat SO TRANSGENIC RESEARCH LA English DT Article DE FISH; Transgene distribution; Particle bombardment-mediated co-transformation; Transgenic wheat ID IN-SITU HYBRIDIZATION; INTEGRATION SITES; CELL-CYCLE; CHROMOSOME REARRANGEMENTS; DNA TRANSFER; BREAD WHEAT; D-GENOME; EXPRESSION; GENE; INTERPHASE AB Although particle bombardment is the predominant method of foreign DNA direct transfer, whether transgene is integrated randomly into the genome has not been determined. In this study, we identified the distribution of transgene loci in 45 transgenic wheat (Triticum aestivum L.) lines containing co-transformed high molecular weight glutenin subunit genes and the selectable marker bar using fluorescence in situ hybridization. Transgene loci were shown to distribute unevenly throughout the genome and incorporate into different locations along individual chromosomes. There was only a slight tendency towards the localization of transgenes in distal chromosome regions. High proportions of transgenes in separate plasmids integrated at the same site and only 7 lines had 2 or 3 loci. Such loci may not segregate frequently in subsequent generations so it is difficult to remove selectable markers from transgenic lines after regeneration. We also found that three transgene lines were associated with rearranged chromosomes, suggesting a the close relationship between particle bombardment-mediated transgene integration and chromosomal rearrangements. C1 [Han, Yonghua] Jiangsu Normal Univ, Sch Life Sci, Xuzhou 221116, Peoples R China. [Han, Yonghua; Wang, Daowen] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China. [Blechl, Ann] USDA ARS, Western Reg Res Ctr, Crop Improvement & Genet, Albany, CA 94710 USA. RP Han, YH (reprint author), Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China. EM hanyonghua@jsnu.edu.cn; dwwang@genetics.ac.cn FU China Postdoctoral Science Foundation [20090460049, 201003181]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) FX This research was supported by the financial support from China Postdoctoral Science Foundation to Yonghua Han (20090460049 and 201003181) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). NR 40 TC 0 Z9 0 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD DEC PY 2015 VL 24 IS 6 BP 1055 EP 1063 DI 10.1007/s11248-015-9906-4 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CW5AM UT WOS:000365007800011 PM 26405007 ER PT J AU Bettez, ND Duncan, JM Groffman, PM Band, LE O'Neil-Dunne, J Kaushal, SS Belt, KT Law, N AF Bettez, Neil D. Duncan, Jonathan M. Groffman, Peter M. Band, Lawrence E. O'Neil-Dunne, Jarlath Kaushal, Sujay S. Belt, Kenneth T. Law, Neely TI Climate Variation Overwhelms Efforts to Reduce Nitrogen Delivery to Coastal Waters SO ECOSYSTEMS LA English DT Article DE Nitrogen cycling; Urban systems; Watershed; Total maximum daily load ID NORTHEASTERN UNITED-STATES; ARID URBAN WATERSHEDS; LAND-USE; STREAM RESTORATION; NUTRIENT EXPORT; RETENTION; DENITRIFICATION; VARIABILITY; GRADIENT; BALANCE AB We calculated watershed nitrogen (N) retention (inputs-outputs)/inputs) each year from 1999-2013 for nine sub-watersheds along an urban-rural gradient near Baltimore MD to determine how land use and climate influence watershed N flux. Retention is critical to efforts to control coastal eutrophication through regulatory efforts that mandate reductions in the total maximum daily load (TMDL) of N that specific water bodies can receive. Retention decreased with urbanization as well as with increases in precipitation with retention decreasing from an average of 91% in the forested sub-watershed to 16% in the most urban sub-watershed. Export was 23% higher, and retention was 7% lower in winter (November-April) than during the growing season. Total N delivery to Baltimore Harbor varied almost threefold between wet and dry years, which is significant relative to the total annual export allowed for all non-point sources to the harbor under the TMDL. These results suggest that expectations for TMDLs should consider watershed land use and climate variability, and their potential for change if they are to result in improvements in receiving water quality. C1 [Bettez, Neil D.; Groffman, Peter M.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Duncan, Jonathan M.; Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC 27599 USA. [Duncan, Jonathan M.; Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC 27599 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Spatial Anal Lab, Burlington, VT 05405 USA. [Kaushal, Sujay S.] Univ Maryland, Dept Geol, College Pk, MD 20742 USA. [Kaushal, Sujay S.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Belt, Kenneth T.] US Forest Serv, USDA, Baltimore, MD 21228 USA. [Law, Neely] Ctr Watershed Protect, Ellicott City, MD 21043 USA. RP Bettez, ND (reprint author), Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. EM bettezn@caryinstitute.org FU U.S. National Science Foundation: NSF-EAR award [0847838]; U.S. National Science Foundation: NSF-DEB award [1027188] FX The data for this paper are available at the Baltimore Ecosystem Web site (http://www.beslter.org/). This work was funded by the U.S. National Science Foundation: NSF-EAR award #0847838 to N.D.B and NSF-DEB award #1027188 (Baltimore LTER). Special thanks to Dan Dillon, who provided valuable assistance and advice in the field, Lisa Martel, Robin Schmidt, Kate Shepard, and Amanda Irish who processed and analyzed samples in the laboratory and two anonymous reviewers who provided two rounds of valuable and constructive comments and suggestions. NR 58 TC 2 Z9 2 U1 4 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD DEC PY 2015 VL 18 IS 8 BP 1319 EP 1331 DI 10.1007/s10021-015-9902-9 PG 13 WC Ecology SC Environmental Sciences & Ecology GA CW6GS UT WOS:000365096400002 ER PT J AU Martinuzzi, S Gavier-Pizarro, GI Lugo, AE Radeloff, VC AF Martinuzzi, Sebastian Gavier-Pizarro, Gregorio I. Lugo, Ariel E. Radeloff, Volker C. TI Future Land-Use Changes and the Potential for Novelty in Ecosystems of the United States SO ECOSYSTEMS LA English DT Article DE novel ecosystems; land-use change; global change; biodiversity; Anthropocene; scenarios ID GLOBAL CHANGE; SOUTHERN WISCONSIN; FOREST COMPOSITION; SPECIES RICHNESS; PUERTO-RICO; PLANT; INVASION; CONSERVATION; MANAGEMENT; TRANSFORMATION AB Rapid global changes due to changing land use, climate, and non-native species are altering environmental conditions, resulting in more novel communities with unprecedented species combinations. Understanding how future anthropogenic changes may affect novelty in ecosystems is important to advance environmental management and ecological research in the Anthropocene. The main goal of this study was to understand how alternative scenarios of future land-use change may affect novelty in ecosystems throughout the conterminous United States. We used five spatially explicit scenarios of future land-use changes, reflecting different land-use policies and changes in agricultural markets, to quantify and map potential drivers of novelty. Our results showed large areas where future land-use changes may increase novelty in ecosystems. The major land-use changes known to increase novelty, including land abandonment and land-use expansion, were widespread in all scenarios (73 million to 95 million ha), especially in the eastern U.S. and along the West Coast. Our scenarios revealed that, at broad scales, future land-use changes will increase novelty in ecosystems, and that traditional conservation policies may have limited ability to prevent the process. In places such as the eastern U.S., conserving and maintaining historical conditions and associated biological diversity may become increasingly difficult due to future land-use changes and related ecological factors. Successful biodiversity conservation and environmental management in the Anthropocene will require novel conservation approaches to be relevant in areas with high levels of novelty in ecosystems. C1 [Martinuzzi, Sebastian; Radeloff, Volker C.] Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, Madison, WI 53706 USA. [Gavier-Pizarro, Gregorio I.] Inst Nacl Tecnol Agr, Inst Recursos Biol, Ctr Invest Recursos Nat, Buenos Aires, DF, Argentina. [Lugo, Ariel E.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. RP Martinuzzi, S (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, SILVIS Lab, 1630 Linden Dr, Madison, WI 53706 USA. EM martinuzzi@wisc.edu RI Radeloff, Volker/B-6124-2016 OI Radeloff, Volker/0000-0001-9004-221X FU National Science Foundation's Coupled Natural-Human Systems Program; Integrative Graduate Education and Research Traineeship (IGERT) award FX We gratefully acknowledge support for this research from the National Science Foundation's Coupled Natural-Human Systems Program and an Integrative Graduate Education and Research Traineeship (IGERT) award to the University of Wisconsin-Madison, and the comments from two anonymous reviewers, which greatly improved this manuscript. Work at the U.S. Forest Service in Puerto Rico was done in collaboration with the University of Puerto Rico. NR 52 TC 3 Z9 3 U1 11 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD DEC PY 2015 VL 18 IS 8 BP 1332 EP 1342 DI 10.1007/s10021-015-9901-x PG 11 WC Ecology SC Environmental Sciences & Ecology GA CW6GS UT WOS:000365096400003 ER PT J AU Bixby, RJ Cooper, SD Gresswell, RE Brown, LE Dahm, CN Dwire, KA AF Bixby, Rebecca J. Cooper, Scott D. Gresswell, Robert E. Brown, Lee E. Dahm, Clifford N. Dwire, Kathleen A. TI Fire effects on aquatic ecosystems: an assessment of the current state of the science SO FRESHWATER SCIENCE LA English DT Article DE wildfire; aquatic ecosystems; streams; rivers; wetlands; ecosystem; biota; prescribed burns ID AFRICAN MOUNTAIN STREAM; WESTERN UNITED-STATES; NORTH-AMERICA; RIPARIAN AREAS; WATER-QUALITY; NATIONAL-PARK; WILDFIRE; USA; CALIFORNIA; MANAGEMENT AB Fire is a prevalent feature of many landscapes and has numerous and complex effects on geological, hydrological, ecological, and economic systems. In some regions, the frequency and intensity of wildfire have increased in recent years and are projected to escalate with predicted climatic and landuse changes. In addition, prescribed burns continue to be used in many parts of the world to clear vegetation for development projects, encourage desired vegetation, and reduce fuel loads. Given the prevalence of fire on the landscape, authors of papers in this special series examine the complexities of fire as a disturbance shaping freshwater ecosystems and highlight the state of the science. These papers cover key aspects of fire effects that range from vegetation loss and recovery in watersheds to effects on hydrology and water quality with consequences for communities (from algae to fish), food webs, and ecosystem processes (e.g., organic matter subsidies, nutrient cycling) across a range of scales. The results presented in this special series of articles expand our knowledge of fire effects in different biomes, water bodies, and geographic regions, encompassing aquatic population, community, and ecosystem responses. In this overview, we summarize each paper and emphasize its contributions to knowledge on fire ecology and freshwater ecosystems. This overview concludes with a list of 7 research foci that are needed to further our knowledge of fire effects on aquatic ecosystems, including research on: 1) additional biomes and geographic regions; 2) additional habitats, including wetlands and lacustrine ecosystems; 3) different fire severities, sizes, and spatial configurations; and 4) additional response variables (e.g., ecosystem processes) 5) over long (> 5 y) time scales 6) with more rigorous study designs and data analyses, and 7) consideration of the effects of fire management practices and policies on aquatic ecosystems. C1 [Bixby, Rebecca J.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Bixby, Rebecca J.] Univ New Mexico, Museum Southwestern Biol, Albuquerque, NM 87131 USA. [Cooper, Scott D.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Cooper, Scott D.] Univ Calif Santa Barbara, Inst Marine Sci, Santa Barbara, CA 93106 USA. [Gresswell, Robert E.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Brown, Lee E.] Univ Leeds, Sch Geog, Leeds LS2 9JT, W Yorkshire, England. [Brown, Lee E.] Univ Leeds, Water Leeds, Leeds LS2 9JT, W Yorkshire, England. [Dahm, Clifford N.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Dwire, Kathleen A.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Bixby, RJ (reprint author), Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. EM bbixby@unm.edu; scott.cooper@lifesci.ucsb.edu; bgresswell@usgs.gov; l.brown@leeds.ac.uk; cdahm@sevilleta.unm.edu; kadwire@fs.fed.us OI Brown, Lee/0000-0002-2420-0088 FU New Mexico Experimental Program to Stimulate Competitive Research (National Science Foundation [NSF]); NSF's Rapid Response and Long-Term Ecological Research programs; EMBER (Effects of Moorland Burning on the Ecohydrology of River basins) project - UK's Natural Environment Research Council [NE/G00224X/1] FX Our gratitude is extended to Editor Pamela Silver and Editorial Assistant Sheila Storms for their invaluable assistance in organizing this special issue on fire effects. Blake Hossack provided valuable comments. We thank Sheila Wiseman for drafting the figure. RJB and CND acknowledge funding through the New Mexico Experimental Program to Stimulate Competitive Research (National Science Foundation [NSF]). SDC was supported by funds from the NSF's Rapid Response and Long-Term Ecological Research programs. LEB's contribution was supported via the EMBER (Effects of Moorland Burning on the Ecohydrology of River basins) project funded by the UK's Natural Environment Research Council (NE/G00224X/1). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 93 TC 1 Z9 1 U1 19 U2 90 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD DEC PY 2015 VL 34 IS 4 BP 1340 EP 1350 DI 10.1086/684073 PG 11 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CW2IM UT WOS:000364815000013 ER PT J AU Rapicavoli, JN Kinsinger, N Perring, TM Backus, EA Shugart, HJ Walker, S Roper, MC AF Rapicavoli, Jeannette N. Kinsinger, Nichola Perring, Thomas M. Backus, Elaine A. Shugart, Holly J. Walker, Sharon Roper, M. Caroline TI O Antigen Modulates Insect Vector Acquisition of the Bacterial Plant Pathogen Xylella fastidiosa SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ESCHERICHIA-COLI; POROUS-MEDIA; ADHESION; TRANSMISSION; COLONIZATION; VIRULENCE; TRANSPORT; LIPOPOLYSACCHARIDES; EXOPOLYSACCHARIDE; MECHANISMS AB Hemipteran insect vectors transmit the majority of plant pathogens. Acquisition of pathogenic bacteria by these piercing/sucking insects requires intimate associations between the bacterial cells and insect surfaces. Lipopolysaccharide (LPS) is the predominant macromolecule displayed on the cell surface of Gram-negative bacteria and thus mediates bacterial interactions with the environment and potential hosts. We hypothesized that bacterial cell surface properties mediated by LPS would be important in modulating vector-pathogen interactions required for acquisition of the bacterial plant pathogen Xylella fastidiosa, the causative agent of Pierce's disease of grapevines. Utilizing a mutant that produces truncated O antigen (the terminal portion of the LPS molecule), we present results that link this LPS structural alteration to a significant decrease in the attachment of X. fastidiosa to blue-green sharpshooter foreguts. Scanning electron microscopy confirmed that this defect in initial attachment compromised subsequent biofilm formation within vector foreguts, thus impairing pathogen acquisition. We also establish a relationship between O antigen truncation and significant changes in the physiochemical properties of the cell, which in turn affect the dynamics of X. fastidiosa adhesion to the vector foregut. Lastly, we couple measurements of the physiochemical properties of the cell with hydrodynamic fluid shear rates to produce a Comsol model that predicts primary areas of bacterial colonization within blue-green sharpshooter foreguts, and we present experimental data that support the model. These results demonstrate that, in addition to reported protein adhesin-ligand interactions, O antigen is crucial for vector-pathogen interactions, specifically in the acquisition of this destructive agricultural pathogen. C1 [Rapicavoli, Jeannette N.; Roper, M. Caroline] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Kinsinger, Nichola; Walker, Sharon] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA. [Perring, Thomas M.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Backus, Elaine A.] ARS, USDA, USDA ARS San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. [Shugart, Holly J.] Univ Florida, Dept Entomol, Citrus Res & Educ Ctr, Lake Alfred, FL USA. RP Roper, MC (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. EM mcroper@ucr.edu FU California Department of Food and Agriculture Pierce's Disease; Glassy-Winged Sharpshooter Program; National Institute of Food and Agriculture, U.S. Department of Agriculture [2013-67012-21277] FX Funding for this project was provided by the California Department of Food and Agriculture Pierce's Disease and Glassy-Winged Sharpshooter Program. This material is based on work supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award 2013-67012-21277 (awarded to Nichola Kinsinger). NR 44 TC 2 Z9 2 U1 0 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD DEC PY 2015 VL 81 IS 23 BP 8145 EP 8154 DI 10.1128/AEM.02383-15 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CW1TY UT WOS:000364775700019 PM 26386068 ER PT J AU Miller, B Anfora, G Buffington, M Daane, KM Dalton, DT Hoelmer, KM Stacconi, MVR Grassi, A Ioriatti, C Loni, A Miller, JC Ouantar, M Wang, XG Wiman, NG Walton, VM AF Miller, Betsey Anfora, Gianfranco Buffington, Matt Daane, Kent M. Dalton, Daniel T. Hoelmer, Kim M. Stacconi, M. Valerio Rossi Grassi, Alberto Ioriatti, Claudio Loni, Augusto Miller, Jeffrey C. Ouantar, M'bark Wang, Xingeng Wiman, Nik G. Walton, Vaughn M. TI Seasonal occurrence of resident parasitoids associated with Drosophila suzukii in two small fruit production regions of Italy and the USA SO BULLETIN OF INSECTOLOGY LA English DT Article DE Pachycrepoideus vindemiae; Trichopria drosophilae; Leptopilina heterotoma; parasitism; biological control; invasive pest ID SPOTTED-WING DROSOPHILA; DIPTERA DROSOPHILIDAE; LARVAL PARASITOIDS; ASOBARA-JAPONICA; UNITED-STATES; PEST; HYMENOPTERA; INFESTATION; INVASION; FLIES AB For the first time we report the results of a survey to determine the presence, seasonal phenology and biological control status of indigenous parasitoid populations utilizing Drosophila suzukii (Matsumura) and Drosophila melanogaster Meigen (Diptera Drosophilidae) as hosts in Trento Province, Northern Italy, and the Willamette Valley, Oregon, USA. Larval and pupal parasitoids were sampled using sentinel traps baited with larvae of D. suzukii or D. melanogaster, or traps baited with fruit or yeast-based host substrates. Two generalist parasitoids, Pachycrepoideus vindemiae (Rondani) (Hymenoptera Pteromalidae) and Leptopilina heterotoma (Thomson) (Hymenoptera Figitidae) emerged from the sentinel traps in both regions, and a third generalist parasitoid, Trichopria drosophilae Perkins (Hymenoptera Diapriidae), was found in Italy. L. heterotoma was present during the early portion of the season in Italy while P. vindemiae was found throughout the growing season in both production regions. Low numbers of parasitoids relative to initial larval load in baits suggest a limited effect of indigenous parasitoids on D. suzukii in these two important fruit production regions. These findings highlight the need for improved biological control of D. suzukii through introduction or augmentation of specialist parasitoids from the native range of D. suzukii. This report provides baseline data on the current status of biological control of D. suzukii in Italy and Oregon. C1 [Miller, Betsey; Dalton, Daniel T.; Miller, Jeffrey C.; Wiman, Nik G.; Walton, Vaughn M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Anfora, Gianfranco; Stacconi, M. Valerio Rossi; Grassi, Alberto; Ioriatti, Claudio; Ouantar, M'bark] Fdn E Mach, Res & Innovat Ctr, I-38010 San Michele All Adige, TN, Italy. [Anfora, Gianfranco; Stacconi, M. Valerio Rossi; Grassi, Alberto; Ioriatti, Claudio; Ouantar, M'bark] Fdn E Mach, Technol Transfer Ctr, I-38010 San Michele All Adige, TN, Italy. [Buffington, Matt] USDA ARS, Systemat Entomol Lab, Washington, DC 20013 USA. [Daane, Kent M.; Wang, Xingeng] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Hoelmer, Kim M.] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Loni, Augusto] Univ Pisa, Dept Agr Food Resources & Environm, I-56124 Pisa, Italy. [Ouantar, M'bark] CIHEAM, Mediterranean Agron Inst Bari, Plant Protect Organ Agr, I-70010 Valenzano, BA, Italy. RP Walton, VM (reprint author), Oregon State Univ, Dept Hort, 4017 ALS Bldg, Corvallis, OR 97331 USA. EM vaughn.walton@oregonstate.edu OI ANFORA, Gianfranco/0000-0003-2545-1409 FU USDA-NIFA [2010-51181-21167]; Autonomous Province of Trento (Italy) FX Funding for research in the US was provided from the USDA-NIFA award # 2010-51181-21167 and in Italy this research was partially funded by the Autonomous Province of Trento (Italy). Research funds for Grandi Progetti, Project LExEM (Laboratory of excellence for epidemiology and modeling, http://www.lexem.eu). Riki York provided technical assistance. USDA is an equal opportunity employer. NR 41 TC 9 Z9 9 U1 4 U2 49 PU ALMA MATER STUDIORUM, UNIV BOLOGNA PI BOLOGNA PA DEPT AGROENVIRONMENTAL SCIENCES & TECHNOLOGY, VIALE G FANIN, 42, BOLOGNA, 40127, ITALY SN 1721-8861 J9 B INSECTOL JI Bull. Insectology PD DEC PY 2015 VL 68 IS 2 BP 255 EP 263 PG 9 WC Entomology SC Entomology GA CV7XZ UT WOS:000364491800012 ER PT J AU Rodhouse, TJ Ormsbee, PC Irvine, KM Vierling, LA Szewczak, JM Vierling, KT AF Rodhouse, Thomas J. Ormsbee, Patricia C. Irvine, Kathryn M. Vierling, Lee A. Szewczak, Joseph M. Vierling, Kerri T. TI Establishing conservation baselines with dynamic distribution models for bat populations facing imminent decline SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE Bayesian hierarchical model; Chiroptera; keystone structures; life history; spatio-temporal variation; species distribution modelling; species-energy theory; trend; turnover ID WIND ENERGY FACILITIES; WHITE-NOSE SYNDROME; LONG-LEGGED MYOTIS; PACIFIC-NORTHWEST; UNITED-STATES; DAY ROOSTS; NORTHERN CALIFORNIA; OCCUPANCY MODELS; EXTINCTION RISK; CENTRAL OREGON AB Aim Bat mortality rates from white-nose syndrome and wind power development are unprecedented. Cryptic and wide-ranging behaviours of bats make them difficult to survey, and population estimation is often intractable. We advance a model-based framework for making spatially explicit predictions about summertime distributions of bats from capture and acoustic surveys. Motivated by species-energy and life-history theory, our models describe hypotheses about spatio-temporal variation in bat distributions along environmental gradients and life-history attributes, providing a statistical basis for conservation decision-making. Location Oregon and Washington, USA. Methods We developed Bayesian hierarchical models for 14 bat species from an 8-year monitoring dataset across a similar to 430,000km(2) study area. Models accounted for imperfect detection and were temporally dynamic. We mapped predicted occurrence probabilities and prediction uncertainties as baselines for assessing future declines. Results Forest cover, snag abundance and cliffs were important predictors for most species. Species occurrence patterns varied along elevation and precipitation gradients, suggesting a potential hump-shaped diversity-productivity relationship. Annual turnover in occurrence was generally low, and occurrence probabilities were stable among most species. We found modest evidence that turnover covaried with the relative riskiness of bat roosting and migration. The fringed myotis (Myotis thysanodes), canyon bat (Parastrellus hesperus) and pallid bat (Antrozous pallidus) were rare; fringed myotis occurrence probabilities declined over the study period. We simulated anticipated declines to demonstrate that mapped occurrence probabilities, updated over time, provide an intuitive way to assess bat conservation status for a broad audience. Main conclusions Landscape keystone structures associated with roosting habitat emerged as regionally important predictors of bat distributions. The challenges of bat monitoring have constrained previous species distribution modelling efforts to temporally static presence-only approaches. Our approach extends to broader spatial and temporal scales than has been possible in the past for bats, making a substantial increase in capacity for bat conservation. C1 [Rodhouse, Thomas J.] Upper Columbia Basin Network, Natl Pk Serv, Bend, OR 97701 USA. [Ormsbee, Patricia C.] US Forest Serv, Bur Land Management Reg, Springfield, OR 97477 USA. [Irvine, Kathryn M.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Vierling, Lee A.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Szewczak, Joseph M.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. [Vierling, Kerri T.] Univ Idaho, Dept Fish & Wildlife, Moscow, ID 83844 USA. RP Rodhouse, TJ (reprint author), Upper Columbia Basin Network Inventory & Monitori, Natl Pk Serv, 63095 Deschutes Market Rd, Bend, OR 97701 USA. EM tom_rodhouse@nps.gov RI Vierling, Kerri/N-6653-2016; OI Rodhouse, Thomas/0000-0001-5953-9113 FU U.S. Forest Service; Bureau of Land Management; Department of Defense Legacy Program; National Park Service FX Funding for the Bat Grid was provided by the U.S. Forest Service, Bureau of Land Management, and Department of Defense Legacy Program. The National Park Service provided additional funding and support. We thank the contributors to the Bat Grid. L. Cousineau provided data management support. J. Hobson provided GIS support. J. Sauer provided helpful comments during preparation of this manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 64 TC 2 Z9 2 U1 17 U2 104 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD DEC PY 2015 VL 21 IS 12 BP 1401 EP 1413 DI 10.1111/ddi.12372 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CV5TM UT WOS:000364334100004 ER PT J AU Gaither, CJ AF Gaither, Cassandra Johnson TI Smokestacks, Parkland, and Community Composition: Examining Environmental Burdens and Benefits in Hall County, Georgia, USA SO ENVIRONMENT AND BEHAVIOR LA English DT Article DE environmental justice; equity; Latino migration; parkland access ID ECOSYSTEM SERVICES; GREEN SPACE; JUSTICE; SOUTH; POLLUTION; REGION; IMPACT; RACE AB This case study addresses environmental equity, in terms of African American, Latino, White, and poor communities' proximity to both industrial facilities and parkland in Hall County, Georgia, USA. The project's two primary goals are to (a) expand environmental justice analyses to account for both environmental burdens (industrial sites) and benefits (parkland acreage), and (b) extend this broader investigation to the county's emergent Latino populations. Results show that both Blacks and Latinos are overrepresented in census block groups (CBGs) within 1 mile of industrial facilities, while Whites are underrepresented. Conversely, Latinos and those near or below poverty are, on average, underrepresented in communities within one-quarter mile of parkland, but Whites are overrepresented. This article discusses the environmental justice and planning implications of these findings in terms of converting existing land uses to urban green space and fuller participation of minorities in such decision making. C1 [Gaither, Cassandra Johnson] US Forest Serv, Southern Res Stn, Athens, GA 30602 USA. RP Gaither, CJ (reprint author), US Forest Serv, Forestry Sci Lab, 320 Green St, Athens, GA 30602 USA. EM cjohnson09@fs.fed.us NR 38 TC 0 Z9 0 U1 7 U2 8 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0013-9165 EI 1552-390X J9 ENVIRON BEHAV JI Environ. Behav. PD DEC PY 2015 VL 47 IS 10 BP 1127 EP 1146 DI 10.1177/0013916514546744 PG 20 WC Environmental Studies; Psychology, Multidisciplinary SC Environmental Sciences & Ecology; Psychology GA CW1MC UT WOS:000364753300004 ER PT J AU Peng, W Zheng, WP Handler, AM Zhang, HY AF Peng, Wei Zheng, Wenping Handler, Alfred M. Zhang, Hongyu TI The role of the transformer gene in sex determination and reproduction in the tephritid fruit fly, Bactrocera dorsalis (Hendel) SO GENETICA LA English DT Article DE Bactrocera dorsalis (Hendel); Transformer gene; Transformer-2 gene; Bdtra RNAi; Tephritid sex determination ID DROSOPHILA-MELANOGASTER; CERATITIS-CAPITATA; ANASTREPHA-SUSPENSA; DIPTERAN INSECTS; RNA INTERFERENCE; DIFFERENTIATION; DOUBLESEX; EXPRESSION; EVOLUTION; ENHANCER AB Transformer (tra) is a switch gene in the somatic sex-determination hierarchy that regulates sexual dimorphism based on RNA splicing in many insects. In tephritids, a Y-linked male determining gene (M) controls sex in the sex-determination pathway. Here, homologues of Drosophila tra and transformer-2 (tra-2) genes were isolated and characterized in Bactrocera dorsalis (Hendel), one of the most destructive agricultural insect pests in many Asian countries. Two male-specific and one female-specific isoforms of B. dorsalis transformer (Bdtra) were identified. The presence of multiple TRA/TRA-2 binding sites in Bdtra suggests that the TRA/TRA-2 proteins are splicing regulators promoting and maintaining, epigenetically, female sex determination by a tra positive feedback loop in XX individuals during development. The expression patterns of female-specific Bdtra transcripts during early embryogenesis shows that a peak appears at 15 h after egg laying. Using dsRNA to knock-down Bdtra expression in the embryo and adult stages, we showed that sexual formation is determined early in the embryo stage and that parental RNAi does not lead to the production of all male progeny as in Tribolium castaneum. RNAi results from adult abdominal dsRNA injections show that Bdtra has a positive influence on female yolk protein gene (Bdyp1) expression and fecundity. C1 [Peng, Wei; Zheng, Wenping; Zhang, Hongyu] Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Hubei Key Lab Insect Resource Applicat & Sustaina, Wuhan 430070, Peoples R China. [Peng, Wei; Zheng, Wenping; Zhang, Hongyu] Huazhong Agr Univ, Coll Plant Sci & Technol, Inst Urban & Hort Entomol, Wuhan 430070, Peoples R China. [Handler, Alfred M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Zhang, HY (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, State Key Lab Agr Microbiol, Hubei Key Lab Insect Resource Applicat & Sustaina, Wuhan 430070, Peoples R China. EM hongyu.zhang@mail.hzau.edu.cn FU earmarked fund for the China Agricultural Research System [CARS-27]; Fundamental Research Funds for the Central Universities [2014PY005] FX This research was supported by the earmarked fund for the China Agricultural Research System (No. CARS-27) and Fundamental Research Funds for the Central Universities (No. 2014PY005). NR 44 TC 3 Z9 3 U1 8 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD DEC PY 2015 VL 143 IS 6 BP 717 EP 727 DI 10.1007/s10709-015-9869-7 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA CV8VE UT WOS:000364565300008 PM 26481008 ER PT J AU Elliott, KJ Miniat, CF Pederson, N Laseter, SH AF Elliott, Katherine J. Miniat, Chelcy F. Pederson, Neil Laseter, Stephanie H. TI Forest tree growth response to hydroclimate variability in the southern Appalachians SO GLOBAL CHANGE BIOLOGY LA English DT Article DE diffuse-porous; hydroclimate; productivity; radial growth; ring-porous; small storms; structural equation modeling; tree-rings ID NORTHERN RED OAK; DROUGHT SEVERITY INDEX; VAPOR-PRESSURE DEFICIT; EASTERN UNITED-STATES; CLIMATE-CHANGE; WATER TRANSPORT; RADIAL GROWTH; STOMATAL SENSITIVITY; DECIDUOUS FOREST; PINUS-ELLIOTTII AB Climate change will affect tree species growth and distribution; however, under the same climatic conditions species may differ in their response according to site conditions. We evaluated the climate-driven patterns of growth for six dominant deciduous tree species in the southern Appalachians. We categorized species into two functional groups based on their stomatal regulation and xylem architecture: isohydric, diffuse porous and anisohydric, ring porous. We hypothesized that within the same climatic regime: (i) species-specific differences in growth will be conditional on topographically mediated soil moisture availability; (ii) in extreme drought years, functional groups will have markedly different growth responses; and (iii) multiple hydroclimate variables will have direct and indirect effects on growth for each functional group. We used standardized tree-ring chronologies to examine growth of diffuse-porous (Acer, Liriodendron, and Betula) and ring-porous (Quercus) species vs. on-site climatic data from 1935 to 2003. Quercus species growing on upslope sites had higher basal area increment (BAI) than Quercus species growing on mesic, cove sites; whereas, Acer and Liriodendron had lower BAI on upslope compared to cove sites. Diffuse-porous species were more sensitive to climate than ring porous, especially during extreme drought years. Across functional groups, radial growth was more sensitive to precipitation distribution, such as small storms and dry spell length (DSL), rather than the total amount of precipitation. Based on structural equation modeling, diffuse-porous species on upslope sites were the most sensitive to multiple hydroclimate variables (r(2)=0.46), while ring-porous species on upslope sites were the least sensitive (r(2)=0.32). Spring precipitation, vapor pressure deficit, and summer storms had direct effects on summer AET/P, and summer AET/P, growing season small storms and DSL partially explained growth. Decreasing numbers of small storms and extending the days between rainfall events will result in significant growth reduction, even in regions with relatively high total annual rainfall. C1 [Elliott, Katherine J.; Miniat, Chelcy F.; Laseter, Stephanie H.] US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC 28763 USA. [Pederson, Neil] Harvard Univ, Harvard Forest, Petersham, MA 01366 USA. RP Elliott, KJ (reprint author), US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC 28763 USA. EM kelliott@fs.fed.us OI Miniat, Chelcy/0000-0002-3266-9783 FU Coweeta Hydrologic Laboratory, USDA Forest Service, Southern Research Station; Coweeta LTER project - National Science Foundation [DEB-0823293]; Columbia University [7919]; Tree Ring Laboratory of Lamont-Doherty Earth Observatory FX We thank Patsy Clinton, Sarah Butler, Jessie Fly, Jess Riddle, Grace Livingston, and Kristina Liles for assistance in field sampling. Drs. K. Novick and T. Hwang and four anonymous reviewers provided helpful comments on the manuscript. This research was supported by Coweeta Hydrologic Laboratory, USDA Forest Service, Southern Research Station; the Coweeta LTER project funded by National Science Foundation grant DEB-0823293; and the Tree Ring Laboratory of Lamont-Doherty Earth Observatory and Columbia University contribution 7919. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the U.S Department of Agriculture of any product or service. The authors have no conflict of interests as defined under Global Change Biology guidelines. NR 106 TC 5 Z9 5 U1 12 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD DEC PY 2015 VL 21 IS 12 BP 4627 EP 4641 DI 10.1111/gcb.13045 PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CW1UO UT WOS:000364777400028 PM 26195014 ER PT J AU Carvalho, M Baranowski, T Foster, E Santos, O Cardoso, B Rito, A Miguel, JP AF Carvalho, M. A. Baranowski, T. Foster, E. Santos, O. Cardoso, B. Rito, A. Miguel, J. Pereira TI Validation of the Portuguese self-administered computerised 24-hour dietary recall among second-, third- and fourth-grade children SO JOURNAL OF HUMAN NUTRITION AND DIETETICS LA English DT Article DE 24-h dietary recall; children; computer; dietary assessment; lunch observations ID SCHOOL LUNCH RECALLS; 5TH-GRADE STUDENTS; ACCURACY; FOOD; ADOLESCENTS; RELIABILITY; NUTRITION; BREAKFAST AB BackgroundCurrent methods for assessing children's dietary intake, such as interviewer-administered 24-h dietary recall (24-h DR), are time consuming and resource intensive. Self-administered instruments offer a low-cost diet assessment method for use with children. The present study assessed the validity of the Portuguese self-administered, computerised, 24-h DR (PAC24) against the observation of school lunch. MethodsForty-one, 7-10-year-old children from two elementary schools, in Lisbon, were observed during school lunch followed by completion of the PAC24 the next day. Accuracy for reporting items was measured in terms of matches, intrusions and omissions; accuracy for reporting amounts was measured in terms of arithmetic and absolute differences for matches and amounts for omissions and intrusions; and accuracy for reporting items and amounts combined was measured in terms of total inaccuracy. The ratio of the estimated weight of food consumed with the actual weight consumed was calculated along with the limits of agreement using the method of Bland and Altman. ResultsComparison of PAC24 against observations at the food level resulted in values of 67.0% for matches, 11.5% for intrusions and 21.5% for omissions. The mean for total inaccuracy was 3.44 servings. For amounts, accuracy was high for matches (-0.17 and 0.23 servings for arithmetic and absolute differences, respectively) and lower for omissions (0.61 servings) and intrusions (0.55 servings). PAC24 was found to under-estimate the weight of food on average by 32% of actual intake. ConclusionsPAC24 is a lower-burden procedure for both respondents and researchers and, with slight modification, comprises a promising method for assessing diet among children. C1 [Carvalho, M. A.; Santos, O.; Miguel, J. Pereira] Univ Lisbon, Fac Med, Inst Prevent Med & Publ Hlth, P-1649028 Lisbon, Portugal. [Baranowski, T.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Foster, E.] Newcastle Univ, Human Nutr Res Ctr, Inst Hlth & Soc, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Cardoso, B.] Univ Nova Lisboa, Fac Sci & Technol, Ctr Informat & Informat Technol, Lisbon, Portugal. [Rito, A.] Natl Inst Hlth Doutor Ricardo Jorge, Lisbon, Portugal. RP Carvalho, M (reprint author), Univ Lisbon, Fac Med, Inst Med Prevent & Saude Publ, Edificio Egas Moniz,Av Prof Egas Moniz, P-1649028 Lisbon, Portugal. EM mariaanacarvalho@gmail.com OI Baranowski, Tom/0000-0002-0653-2222 FU Department of Health [PDA/03/07/022] NR 30 TC 4 Z9 4 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-3871 EI 1365-277X J9 J HUM NUTR DIET JI J. Hum. Nutr. Diet. PD DEC PY 2015 VL 28 IS 6 BP 666 EP 674 DI 10.1111/jhn.12280 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CV8FQ UT WOS:000364516700013 PM 25420921 ER PT J AU Abernathy, J Panserat, S Welker, T Plagne-Juan, E Sakhrani, D Higgs, DA Audouin, F Devlin, RH Overturf, K AF Abernathy, Jason Panserat, Stephane Welker, Thomas Plagne-Juan, Elisabeth Sakhrani, Dionne Higgs, David A. Audouin, Florence Devlin, Robert H. Overturf, Ken TI Food Shortage Causes Differential Effects on Body Composition and Tissue-Specific Gene Expression in Salmon Modified for Increased Growth Hormone Production SO MARINE BIOTECHNOLOGY LA English DT Article DE Oncorhynchus kisutch; Coho salmon; Transgenic; Liver; Metabolism; Muscle; Proximate analysis; Gene expression; Network analysis ID TROUT ONCORHYNCHUS-MYKISS; TRANSGENIC COHO SALMON; NECROSIS-FACTOR-ALPHA; RAINBOW-TROUT; ATLANTIC SALMON; PROXIMATE COMPOSITION; TNF-ALPHA; FACTOR-I; PROTEIN-DEGRADATION; ENERGY-UTILIZATION AB Growth hormone (GH) transgenic salmon possesses markedly increased metabolic rate, appetite, and feed conversion efficiency, as well as an increased ability to compete for food resources. Thus, the ability of GH-transgenic fish to withstand periods of food deprivation as occurs in nature is potentially different than that of nontransgenic fish. However, the physiological and genetic effects of transgenic GH production over long periods of food deprivation remain largely unknown. Here, GH-transgenic coho salmon (Oncorhynchus kisutch) and nontransgenic, wild-type coho salmon were subjected to a 3-month food deprivation trial, during which time performance characteristics related to growth were measured along with proximate compositions. To examine potential genetic effects of GH-transgenesis on long-term food deprivation, a group of genes related to muscle development and liver metabolism was selected for quantitative PCR analysis. Results showed that GH-transgenic fish lose weight at an increased rate compared to wild-type even though proximate compositions remained relatively similar between the groups. A total of nine genes related to muscle physiology (cathepsin, cee, insulin-like growth factor, myostatin, murf-1, myosin, myogenin, proteasome delta, tumor necrosis factor) and five genes related to liver metabolism (carnitine palmitoyltransferase, fatty acid synthase, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, glucokinase) were shown to be differentially regulated between GH-transgenic and wild-type coho salmon over time. These genetic and physiological responses assist in identifying differences between GH-transgenic and wild-type salmon in relation to fitness effects arising from elevated growth hormone during periods of long-term food shortage. C1 [Abernathy, Jason; Welker, Thomas; Overturf, Ken] USDA ARS, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Panserat, Stephane; Plagne-Juan, Elisabeth] INRA, Nutr Metab Aquaculture UR1067, F-64310 St Pee Sur Nivelle, France. [Sakhrani, Dionne; Higgs, David A.; Audouin, Florence; Devlin, Robert H.] Fisheries & Oceans Canada, W Vancouver, BC V7V 1N6, Canada. RP Overturf, K (reprint author), USDA ARS, Hagerman Fish Culture Expt Stn, 3059F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. EM Ken.Overturf@ars.usda.gov OI Abernathy, Jason/0000-0001-6084-8239; Panserat, Stephane/0000-0002-4479-9868 FU USDA-Agricultural Research Service; INRA-National Institute of Agronomic Research; Canadian Biotechnology Strategy FX This work was in part funded by the USDA-Agricultural Research Service to KO, by INRA-National Institute of Agronomic Research to SP and from the Canadian Biotechnology Strategy to RHD. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 66 TC 1 Z9 1 U1 5 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-2228 EI 1436-2236 J9 MAR BIOTECHNOL JI Mar. Biotechnol. PD DEC PY 2015 VL 17 IS 6 BP 753 EP 767 DI 10.1007/s10126-015-9654-8 PG 15 WC Biotechnology & Applied Microbiology; Marine & Freshwater Biology SC Biotechnology & Applied Microbiology; Marine & Freshwater Biology GA CV8EJ UT WOS:000364512500006 PM 26265485 ER PT J AU Ren, L Zhang, D Chen, GQ Reed, BM Shen, XH Chen, HY AF Ren, Li Zhang, Di Chen, Guan-qun Reed, Barbara M. Shen, Xiao-hui Chen, Huo-ying TI Transcriptomic profiling revealed the regulatory mechanism of Arabidopsis seedlings response to oxidative stress from cryopreservation SO PLANT CELL REPORTS LA English DT Article DE Arabidopsis thaliana; Cryopreservation; Stress physiology; Transcriptomics; Oxidative stress; Reactive oxygen species ID PROGRAMMED CELL-DEATH; ANTISTRESS COMPOUNDS IMPROVE; REACTIVE OXYGEN PRODUCTION; ABIOTIC STRESS; ABSCISIC-ACID; PLANT CRYOPRESERVATION; ANTIOXIDANT RESPONSE; TISSUE CULTURES; SOMATIC EMBRYOS; GENE-EXPRESSION AB Elevated antioxidant status and positive abiotic stress response in dehydration enhance cell resistance to cryoinjury, and controlling oxidative damage via reactive oxygen species homeostasis maintenance leads to high survival. Cryoprotectants are important for cell survival in cryopreservation, but high concentrations can also cause oxidative stress. Adding vitamin C to the cryoprotectant doubled the survival ratio in Arabidopsis thaliana 60-h seedlings (seedlings after 60-h germination) cryopreservation. In this study, the metabolites and transcriptional profiling of 60-h seedlings were analyzed in both the control cryopreservation procedure (CCP) and an improved cryopreservation procedure (ICP) to reveal the mechanism of plant cell response to oxidative stress from cryopreservation. Reactive oxygen species (ROS) and peroxidation levels reached a peak after rapid cooling-warming in CCP, which were higher than that in ICP. In addition, gene regulation was significantly increased in CCP and decreased in ICP during rapid cooling-warming. Before cryogenic treatment, the number of specifically regulated genes was nearly 10 times higher in ICP dehydration than CCP dehydration. Among these genes, DREBs/CBFs were beneficial to cope with cryoinjury, and calcium-binding protein, OXI1, WRKY and MYB family members as key factors in ROS signal transduction activated the ROS-producing and ROS-scavenging networks including AsA-GSH and GPX cycles involved in scavenging H2O2. Finally, elevated antioxidant status and oxidative stress response in the improved dehydration enhanced seedling resistance to cryogenic treatment, maintained ROS homeostasis and improved cell recovery after cryopreservation. C1 [Ren, Li; Zhang, Di; Chen, Guan-qun; Shen, Xiao-hui; Chen, Huo-ying] Shanghai Jiao Tong Univ, Sch Agr & Biol, Minist Agr, Key Lab Urban Agr South, Shanghai, Peoples R China. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Shen, XH (reprint author), Shanghai Jiao Tong Univ, Sch Agr & Biol, Minist Agr, Key Lab Urban Agr South, 800 Rd Dong Chuan, Shanghai, Peoples R China. EM shenxh62@sjtu.edu.cn; chhy@sjtu.edu.cn FU National Natural Science Foundation of China [31170655, 31300580]; Shanghai Jiao Tong University 'Agri-X' Interdisciplinary Research Foundation [2015003] FX This work was supported by the National Natural Science Foundation of China (No. 31170655 and No. 31300580) and Shanghai Jiao Tong University 'Agri-X' Interdisciplinary Research Foundation (No. 2015003). The authors thank Prof. Hong-Quan Yang (Fudan University, Shanghai, China) for experimental materials. NR 68 TC 3 Z9 3 U1 4 U2 54 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0721-7714 EI 1432-203X J9 PLANT CELL REP JI Plant Cell Reports PD DEC PY 2015 VL 34 IS 12 BP 2161 EP 2178 DI 10.1007/s00299-015-1859-9 PG 18 WC Plant Sciences SC Plant Sciences GA CV8JM UT WOS:000364530300012 PM 26373654 ER PT J AU Mura, M McRoberts, RE Chirici, G Marchetti, M AF Mura, Matteo McRoberts, Ronald E. Chirici, Gherardo Marchetti, Marco TI Estimating and mapping forest structural diversity using airborne laser scanning data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Airborne laser scanning; Forest structural diversity; GREG estimator; Model-assisted estimator ID BIRD SPECIES RICHNESS; STAND STRUCTURE; MONITORING BIODIVERSITY; INVENTORY DATA; LIDAR; ASSEMBLAGES; COMPLEXITY; MANAGEMENT; INDICATORS; ECOSYSTEM AB Among the wide array of terrestrial habitats, forest and wooded lands are the richest from both biological and genetic points of view because of their inherent structural and compositional complexity and diversity. Although species composition is an important biodiversity feature, forest structure may be even more relevant for biodiversity assessments because a diversified structure is likely to have more niches, which in turn, host more species and contribute to a more efficient use of available resources. Structure plays a major role as a diversity indicator for management purposes where maps of forest structural diversity are of great utility when planning conservation strategies. Airborne laser scanning (ALS) data have been demonstrated to be a reliable and valid source of information for describing the three-dimensional structure of forests. Using ALS metrics as predictor variables, we developed regression models for predicting indices of forest structural diversity for a study area in Molise, Italy. The study had two primary objectives: (i) to estimate indices of structural diversity for the entire study area, and (ii) to construct maps depicting the spatial pattern of the structural diversity indices. Our results demonstrate the utility of simple linear models using ALS data for improving areal estimates of mean structural diversity, and the resulting maps capture the patterns of structural diversity in the study area. (C) 2015 Elsevier Inc. All rights reserved. C1 [Mura, Matteo; Marchetti, Marco] Univ Molise, Dipartimento Biosci & Terr, I-86090 Pesche, IS, Italy. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Chirici, Gherardo] Univ Florence, Dept Agr Food & Forestry Syst, I-50145 Florence, Italy. RP Mura, M (reprint author), Univ Molise, Dipartimento Biosci & Terr, I-86090 Pesche, IS, Italy. EM mur.teo@gmail.com NR 100 TC 5 Z9 5 U1 4 U2 28 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD DEC 1 PY 2015 VL 170 BP 133 EP 142 DI 10.1016/j.rse.2015.09.016 PG 10 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CW1CB UT WOS:000364726100012 ER PT J AU Yu, GT Klindworth, DL Friesen, TL Faris, JD Zhong, SB Rasmussen, JB Xu, SS AF Yu, Guotai Klindworth, Daryl L. Friesen, Timothy L. Faris, Justin D. Zhong, Shaobin Rasmussen, Jack B. Xu, Steven S. TI Development of a diagnostic co-dominant marker for stem rust resistance gene Sr47 introgressed from Aegilops speltoides into durum wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID TRITICUM-AESTIVUM L.; CONFERRING RESISTANCE; ASSISTED SELECTION; CONSENSUS MAP; UG99; IDENTIFICATION; LINES; SR2 AB A robust and diagnostic STS marker for stem rust resistance gene Sr47 was developed and validated for marker-assisted selection. Stem rust (caused by Puccinia graminis f. sp. tritici, Pgt) resistance gene Sr47, originally transferred from Aegilops speltoides to durum wheat (Triticum turgidum subsp. durum) line DAS15, confers a high level of resistance to Pgt race TTKSK (Ug99). Recently, the durum Rusty 5D(5B) substitution line was used to reduce the Ae. speltoides segment, and the resulting lines had Sr47 on small Ae. speltoides segments on wheat chromosome arm 2BL. The objective of this study was to develop a robust marker for marker-assisted selection of Sr47. A 200-kb segment of the Brachypodium distachyon genome syntenic with the Sr47 region was used to identify wheat expressed sequence tags (ESTs) homologous to the B. distachyon genes. The wheat EST sequences were then used to develop sequence-tagged site (STS) markers. By analyzing the markers for polymorphism between Rusty and DAS15, we identified a co-dominant STS marker, designated as Xrwgs38, which amplified 175 and 187 bp fragments from wheat chromosome 2B and Ae. speltoides chromosome 2S segments, respectively. The marker co-segregated with the Ae. speltoides segments carrying Sr47 in the families from four BC2F1 plants, including the parent plants for durum lines RWG35 and RWG36 with the pedigree of Rusty/3/Rusty 5D(5B)/DAS15//47-1 5D(5B). Analysis of 62 durum and common wheat cultivars/lines lacking the Sr47 segment indicated that they all possessed the 175-bp allele of Xrwgs38, indicating that it was diagnostic for the small Ae. speltoides segment carrying Sr47. This study demonstrated that Xrwgs38 will facilitate the selection of Sr47 in durum and common wheat breeding. C1 [Yu, Guotai; Zhong, Shaobin; Rasmussen, Jack B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Klindworth, Daryl L.; Friesen, Timothy L.; Faris, Justin D.; Xu, Steven S.] USDA ARS, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. RP Xu, SS (reprint author), USDA ARS, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM steven.xu@ars.usda.gov FU Bill & Melinda Gates Foundation; USDA-ARS CRIS Project [3060-520-037-00D] FX We thank Drs. Chao-Chien Jan and G. Francois Marais for critically reviewing the manuscript. The authors also thank Danielle Holmes for technical support. This research was supported in part by funds to S. S. X. provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project No. 3060-520-037-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 32 TC 1 Z9 1 U1 1 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2015 VL 128 IS 12 BP 2367 EP 2374 DI 10.1007/s00122-015-2590-1 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CV8KJ UT WOS:000364533500002 PM 26260850 ER PT J AU Pirseyedi, SM Somo, M Poudel, RS Cai, XW McCallum, B Saville, B Fetch, T Chao, SM Marais, F AF Pirseyedi, Seyed-Mostafa Somo, Mohamed Poudel, Roshan Sharma Cai, Xiwen McCallum, Brent Saville, Barry Fetch, Thomas Chao, Shiaoman Marais, Francois TI Characterization of recombinants of the Aegilops peregrina-derived Lr59 translocation of common wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID LEAF-RUST RESISTANCE; STEM RUST; TRITICUM-AESTIVUM; AGROPYRON; GENE; CHROMOSOMES; THINOPYRUM AB A compensating, recombined Lr59 translocation with greatly reduced alien chromatin was identified. Microsatellite locus Xdupw217 occurs within the remaining segment and can be used as a co-dominant marker for Lr59. In earlier studies, leaf rust (caused by Puccinia triticina Eriks.) resistance gene Lr59 was transferred from Aegilops peregrina (Hackel) Maire et Weiler to chromosome arm 1AL of common wheat (Triticum aestivum L.). The resistance gene was then genetically mapped on the translocated chromosome segment following homoeologous pairing induction. Eight recombinants that retained the least alien chromatin apparently resulted from crossover within a terminal region of the translocation that was structurally different from 1AL. These recombinants could not be differentiated by size, and it was not clear whether they were compensating in nature. The present study determined that the distal part of the original translocation has group 6 chromosome homoeology and a 6BS telomere (with the constitution of the full translocation chromosome being 1AS center dot 1L(P)center dot 6S(P) center dot 6BS). During the allosyndetic pairing induction experiment to map and shorten the full size translocation, a low frequency of quadrivalents involving 1A, the 1A translocation, and two 6B chromosomes was likely formed. Crossover within such quadrivalents apparently produced comparatively small compensating alien chromatin inserts within the 6BS satellite region on chromosome 6B of seven of the eight recombinants. It appears that the Gli-B2 storage protein locus on 6BS has not been affected by the recombination events, and the translocations are therefore not expected to affect baking quality. Simple sequence repeat marker results showed that Lr59-151 is the shortest recombinant, and it will therefore be used in breeding. Marker DUPW217 detects a homoeo-allele within the remaining alien chromatin that can be used for marker-assisted selection of Lr59. C1 [Pirseyedi, Seyed-Mostafa; Somo, Mohamed; Poudel, Roshan Sharma; Cai, Xiwen; Marais, Francois] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [McCallum, Brent] CRC AAFC, Morden, MB R6M 1Y5, Canada. [Saville, Barry] Trent Univ, Peterborough, ON K9J 7B8C, Canada. [Fetch, Thomas] BRC AAFC, Brandon, MB R7A 5Y3, Canada. [Chao, Shiaoman] ARS, Res Lab, USDA, Fargo, ND 58102 USA. RP Marais, F (reprint author), N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM gideon.marais@ndsu.edu FU North Dakota Wheat Commission; Minnesota Wheat Research and Promotion Council FX We thank the North Dakota Wheat Commission and Minnesota Wheat Research and Promotion Council for financial support. Ms Mary Osenga (US Department of Agriculture, Agricultural Research Service Biosciences Research Laboratory, Fargo, ND 58102) performed the SNP analyses on the F2 mapping population while Mr J Zurn (Plant Pathology department, NDSU) provided valuable advice with the mapping analysis. NR 31 TC 3 Z9 3 U1 0 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2015 VL 128 IS 12 BP 2403 EP 2414 DI 10.1007/s00122-015-2594-x PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CV8KJ UT WOS:000364533500005 PM 26239411 ER PT J AU Wen, CL Mao, AJ Dong, CJ Liu, HY Yu, SC Guo, YD Weng, YQ Xu, Y AF Wen, Changlong Mao, Aijun Dong, Congjuan Liu, Huyu Yu, Shuancang Guo, Yang-Dong Weng, Yiqun Xu, Yong TI Fine genetic mapping of target leaf spot resistance gene cca-3 in cucumber, Cucumis sativus L. SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GENOME-WIDE ANALYSIS; CORYNESPORA-CASSIICOLA; MOLECULAR CHARACTERIZATION; ENCODING GENES; QTL ANALYSIS; 1ST REPORT; MAP; DOMESTICATION; DIVERSITY; CHINA AB The cucumber target leaf spot resistance gene cca - 3 was fine mapped in a 79-kb region harboring a CC-NB-ARC type R gene that may be responsible for the hypersensitive responses to infection of the target leaf spot pathogen in cucumber. The target leaf spot (TLS) is one of the most important foliar diseases in cucumber (Cucumis sativus L.). In this study, we conducted fine genetic mapping of a simply inherited recessive resistance gene, cca-3 against TLS with 193 F-2:3 families and 890 F-2 plants derived from the resistant cucumber inbred line D31 and the susceptible line D5. Initial mapping with microsatellite markers and bulked segregant analysis placed cca-3 in a 2.5-Mbp region of cucumber chromosome 6. The D5 and D31 lines were re-sequenced at 10x genome coverage to explore new markers in the target region. Genetic mapping in the large F-2 population delimited the cca-3 locus in a 79-kb region with flanking markers Indel16874230 and Indel16953846. Additional fine mapping and gene annotation in this region revealed that a CC-NB-ARC type resistance gene analog, Csa6M375730, seems to be the candidate gene for cca-3. One single nucleotide polymorphism (SNP) was found in the NB-ARC domain of this candidate gene sequence between D31 and D5 that may lead to amino acid change, thus altering the function of the conserved NB-ARC motif. This SNP was validated in the segregating population as well as 24 independent cucumber lines. There was significantly higher level of cca-3 expression in the leaves of D5 (susceptible) than in D31 (resistant), and the expression level was positively correlated with the areas of necrotic spots on leaves after inoculation. It seems the cca-3 resistance gene was able to induce hypersensitive responses to the infection by TLS pathogen. C1 [Wen, Changlong; Mao, Aijun; Dong, Congjuan; Liu, Huyu; Yu, Shuancang; Xu, Yong] Beijing Acad Agr & Forestry Sci, BVRC, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Wen, Changlong; Mao, Aijun; Dong, Congjuan; Liu, Huyu; Yu, Shuancang; Xu, Yong] Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China. [Guo, Yang-Dong] China Agr Univ, Coll Agr & Biotechnol, Beijing 100193, Peoples R China. [Weng, Yiqun] Univ Wisconsin, Dept Hort, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Wen, CL (reprint author), Beijing Acad Agr & Forestry Sci, BVRC, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. EM wenchanglong@nercv.org; yaguo@cau.edu.cn FU National Natural Science Foundation of China [31401873]; National Science & Technology Pillar Program [2014BAD01B09, 2012BAD02B03]; National High Technology Research and Development Program of China [2012AA100103]; Beijing Municipal Science and Technology Commission [Z131100003113012]; Beijing Academy of Agricultural and Forestry Sciences [CXJJ201305/QNJJ201501] FX This work was supported in part by grants from the National Natural Science Foundation of China (No. 31401873 to CW), the National Science & Technology Pillar Program (2014BAD01B09 and 2012BAD02B03), the National High Technology Research and Development Program of China (2012AA100103), the Beijing Municipal Science and Technology Commission(Z131100003113012), and Beijing Academy of Agricultural and Forestry Sciences (CXJJ201305/QNJJ201501). NR 47 TC 2 Z9 2 U1 6 U2 41 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD DEC PY 2015 VL 128 IS 12 BP 2495 EP 2506 DI 10.1007/s00122-015-2604-z PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CV8KJ UT WOS:000364533500013 PM 26385372 ER PT J AU Carrera-Martinez, R Aponte-Diaz, L Ruiz-Arocho, J Jenkins, DA AF Carrera-Martinez, Roberto Aponte-Diaz, Laura Ruiz-Arocho, Jorge Jenkins, David A. TI SYMPTOMATOLOGY OF INFESTATION BY HYPOGEOCOCCUS PUNGENS: CONTRASTS BETWEEN HOST SPECIES SO HASELTONIA LA English DT Article DE Hypogeococcus pungens; Harrisia Cactus Mealybug; Puerto Rico; biological invasion; Pilosocereus royenii; symptomatology ID COLUMNAR CACTI AB The Harrisia cactus mealybug (HCM), Hypogeococcus pungens (Hemiptera: Pseucococcidae), is known to be an effective biological control agent for invasive columnar cacti in Australia and South Africa. In the Caribbean, Central and North America, HCM is an alien invasive species that is threatening native cactus. Despite their negative impacts on ecosystems where cacti are native, no publications have described the symptomatology associated with HCM infestation. This article describes the morphology of the galls produced by HCM on seven host cactus species occurring in Puerto Rico. C1 [Carrera-Martinez, Roberto; Aponte-Diaz, Laura; Ruiz-Arocho, Jorge] Univ Puerto Rico, Dept Biol, Mayaguez Campus, Mayaguez, PR 00682 USA. [Jenkins, David A.] USDA ARS, TARS, Mayaguez, PR USA. [Jenkins, David A.] South Carolina Forestry Commiss, Columbia, SC USA. RP Carrera-Martinez, R (reprint author), Univ Puerto Rico, Dept Biol, Mayaguez Campus, Mayaguez, PR 00682 USA. EM roberto.carrera@upr.edu; djenkins@scfc.gov NR 14 TC 0 Z9 0 U1 0 U2 0 PU CACTUS SUCCULENT SOC AMER INC PI PAHRUMP PA 2391 E CACTUS ST, PAHRUMP, NV 89048 USA SN 1070-0048 EI 1938-2898 J9 HASELTONIA JI Haseltonia PD DEC PY 2015 IS 21 BP 14 EP 18 PG 5 WC Plant Sciences SC Plant Sciences GA EE6KW UT WOS:000389720500003 ER PT J AU Vellinga, EC Kuyper, TW Ammirati, J Desjardin, DE Halling, RE Justo, A Laessoe, T Lebel, T Lodge, DJ Matheny, PB Methven, AS Moreau, PA Mueller, GM Noordeloos, ME Nuytinck, J Ovrebo, CL Verbeken, A AF Vellinga, Else C. Kuyper, Thomas W. Ammirati, Joe Desjardin, Dennis E. Halling, Roy E. Justo, Alfredo Laessoe, Thomas Lebel, Teresa Lodge, D. Jean Matheny, P. Brandon Methven, Andrew S. Moreau, Pierre-Arthur Mueller, Gregory M. Noordeloos, Machiel E. Nuytinck, Jorinde Ovrebo, Clark L. Verbeken, Annemieke TI Six simple guidelines for introducing new genera of fungi SO IMA FUNGUS LA English DT Editorial Material DE basidiomycetes; molecular systematics; nomenclature; phylogenetics; taxonomy AB We formulate five guidelines for introducing new genera, plus one recommendation how to publish the results of scientific research. We recommend that reviewers and editors adhere to these guidelines. We propose that the underlying research is solid, and that the results and the final solutions are properly discussed. The six criteria are: (1) all genera that are recognized should be monophyletic; (2) the coverage of the phylogenetic tree should be wide in number of species, geographic coverage, and type species of the genera under study; (3) the branching of the phylogenetic trees has to have sufficient statistical support; (4) different options for the translation of the phylogenetic tree into a formal classification should be discussed and the final decision justified; (5) the phylogenetic evidence should be based on more than one gene; and (6) all supporting evidence and background information should be included in the publication in which the new taxa are proposed, and this publication should be peer-reviewed. C1 [Vellinga, Else C.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Kuyper, Thomas W.] Wageningen Univ, Dept Soil Qual, POB 47, NL-6700 AA Wageningen, Netherlands. [Ammirati, Joe] Univ Washington, Dept Biol, Seattle, WA 98195 USA. [Desjardin, Dennis E.] San Francisco State Univ, Dept Biol, 1600 Holloway Ave, San Francisco, CA 94132 USA. [Halling, Roy E.] New York Bot Garden, Inst Systemat Bot, 2900 Southern Blvd, Bronx, NY 10458 USA. [Justo, Alfredo] Univ Nacl Autonoma Mexico, Inst Biol, Dept Bot, Mexico City 04510, DF, Mexico. [Laessoe, Thomas] Univ Copenhagen, Nat Hist Museum Denmark, Dept Biol, Univ Pk 15, DK-2100 Copenhagen O, Denmark. [Lebel, Teresa] Royal Bot Gardens Victoria, Birdwood Ave, Melbourne, Vic 3004, Australia. [Lodge, D. Jean] USDA Forest Serv, Ctr Forest Mycol Res, Northern Res Stn, Luquillo, PR 00773 USA. [Matheny, P. Brandon] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Methven, Andrew S.] Eastern Illinois Univ, Dept Biol Sci, Charleston, IL 61920 USA. [Moreau, Pierre-Arthur] Univ Lille, Fac Sci Pharmaceut & Biol, Dept Sci Vegetales & Fong, F-59006 Lille, France. [Mueller, Gregory M.] Chicago Bot Garden, 1000 Lake Cook Rd, Glencoe, IL 60022 USA. [Noordeloos, Machiel E.; Nuytinck, Jorinde] Naturalis Biodivers Ctr, POB 9517, NL-2300 RA Leiden, Netherlands. [Ovrebo, Clark L.] Univ Cent Oklahoma, Dept Biol, Edmond, OK 73034 USA. [Verbeken, Annemieke] Univ Ghent, Dept Biol, KL Ledeganckst 35, B-9000 Ghent, Belgium. RP Kuyper, TW (reprint author), Wageningen Univ, Dept Soil Qual, POB 47, NL-6700 AA Wageningen, Netherlands. EM ecvellinga@comcast.net; thom.kuyper@wur.nl NR 38 TC 15 Z9 15 U1 0 U2 0 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD DEC PY 2015 VL 6 IS 2 BP 65 EP 68 PG 4 WC Mycology SC Mycology GA V46UM UT WOS:000209909100018 ER PT J AU Bissett, J Gams, W Jaklitsch, W Samuels, GJ AF Bissett, John Gams, Walter Jaklitsch, Walter Samuels, Gary J. TI Accepted Trichoderma names in the year 2015 SO IMA FUNGUS LA English DT Article DE Hypocrea; Hypocreales; Lists of Protected Names; Nomenclature; Pleomorphic fungi AB A list of 254 names of species and two names of varieties in Trichoderma with name or names against which they are to be protected, following the ICN (Melbourne Code, Art. 14.13), is presented for consideration by the General Committee established by the Congress, which then will refer them to the Nomenclature Committee for Fungi (NCF). This list includes 252 species, one variety and one form. Two new names are proposed: T. neocrassum Samuel (syn. Hypocrea crassa P. Chaverri & Samuels), T. patellotropicum Samuels (syn. Hypocrea patella f. tropica Yoshim. Doi). The following new combinations in Trichoderma are proposed: T. brevipes (Mont.) Samuels, T. cerebriforme (Berk.) Samuels, T. latizonatum (Peck) Samuels, and T. poronioideum (A. Moller) Samuels. The following species are lectotypified: T. americanum (Canham) Jaklitsch & Voglmayr, Gliocladium flavofuscum J.H. Miller, Giddens & A. A. Foster, T. inhamatum Veerkamp & W. Gams, T. konilangbra Samuels, O. Petrini & C.P. Kubicek, T. koningii Oudem., T. pezizoides (Berk. & Broome) Jaklitsch & Voglmayr, T. sulphureum (Schwein.) Jaklitsch & Voglmayr and T. virens (J.H. Miller, Giddens & A. A. Foster) Arx. Epitypes are proposed for the following species: T. albocorneum (Yoshim. Doi) Jaklitsch & Voglmayr, T. albofulvum (Berk. & Broome) Jaklitsch & Voglmayr, T. atrogelatinosum (Dingley) Jaklitsch & Voglmayr, T. corneum (Pat.) Jaklitsch & Voglmayr, T. cornu-damae (Pat.) Z.X. Zhu & W.Y. Zhuang, T. flaviconidium (P. Chaverri, Druzhinina & Samuels) Jaklitsch & Voglmayr, T. hamatum (Bonord.) Bain., T. hunua (Dingley) Jaklitsch & Voglmayr, T. patella (Cooke & Peck) Jaklitsch & Voglmayr, Hypocrea patella f. tropica Yoshim. Doi, T. polysporum (Link) Rifai, T. poronioideum (A. Moller) Samuels T. semiorbis (Berk.) Jaklitsch & Voglmayr, T. sulphureum (Schwein.) Jaklitsch & Voglmayr, and T. tropicosinense (P.G. Liu) P.G. Liu, Z.X. Zhu & W. Y. Zhuang. C1 [Bissett, John] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. [Jaklitsch, Walter] Univ Vienna, Fac Ctr Biodivers, Dept Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Gams, Walter] BOKU Univ Nat Resources & Life Sci, Dept Forest & Soil Sci, Inst Forest Entomol Forest Pathol & Forest Protec, A-1190 Vienna, Austria. [Samuels, Gary J.] ARS, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Samuels, GJ (reprint author), 321 Hedgehog Mt Rd, Deering, NH 03244 USA. EM samuelspatty@gmail.com FU Austrian Science Fund FWF [P 22081] NR 49 TC 10 Z9 10 U1 3 U2 6 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD DEC PY 2015 VL 6 IS 2 BP 263 EP 295 DI 10.5598/imafungus.2015.06.02.02 PG 33 WC Mycology SC Mycology GA V46UM UT WOS:000209909100022 PM 26734542 ER PT J AU Simmons, DR Kepler, RM Rehner, SA Groden, E AF Simmons, D. Rabern Kepler, Ryan M. Rehner, Stephen A. Groden, Eleanor TI Phylogeny of Hirsutella species (Ophiocordycipitaceae) from the USA: remedying the paucity of Hirsutella sequence data SO IMA FUNGUS LA English DT Article DE 18S rDNA; biocontrol; entomopathogenic fungi; Hypocreales; pleomorphic fungi; rpb1; tef1 AB Hirsutella (Ophiocordycipitaceae: Hypocreales) is a genus of insect, mite, and nematode pathogens with an asexual morph, which generally produce a mucilaginous cluster of one or several conidia on phialides that are basally subulate and taper to a fine neck. The generic name Hirsutella has been proposed for suppression in favour of Ophiocordyceps as a consequence of the ending of dual nomenclature for different morphs of pleomorphic fungi in 2011. Though the generic name is well established, geographically dispersed, and speciose, exceptionally few sequences are available in online databases. We examined 46 isolates of 23 Hirsutella species from the USA, curated by the USDA-ARS Collection of Entomopathogenic Fungal Cultures (ARSEF Culture Collection), that previously had not been molecularly characterized and produced a phylogeny of these organisms; we included previously published Hirsutella and Ophiocordyceps taxa. In producing the largest phylogeny of Hirsutella isolates so far, we provide: (1) context for discussing previously-hypothesized relationships; (2) evidence for revisions as taxonomic transitions move forward; and (3) available molecular data to be incorporated into further evolutionary studies of Ophiocordycipitaceae. C1 [Simmons, D. Rabern] Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. [Kepler, Ryan M.; Rehner, Stephen A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Groden, Eleanor] Univ Maine, Sch Biol & Ecol, Orono, ME 04473 USA. RP Simmons, DR (reprint author), Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. EM rabernsimmons@ufl.edu FU Agricultural and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2013-67012-21115]; Maine Agriculture and Forestry Experiment Station at the University of Maine FX We thank Richard A Humber for access to the ARSEF Culture Collection and his expert advice; Patty Singer and Dave Cox of the DNA Sequencing Facility at the University of Maine for their services. We also thank Joyce E Longcore for participation in taxonomic and phylogenetic discussions, and Jerry R Longcore for critical consultation in preparing the manuscript. This project was supported by the Agricultural and Food Research Initiative Competitive Grant No. 2013-67012-21115 from the USDA National Institute of Food and Agriculture, and the Maine Agriculture and Forestry Experiment Station at the University of Maine. This is MAFES Publication No. 3438. NR 50 TC 1 Z9 2 U1 2 U2 2 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD DEC PY 2015 VL 6 IS 2 BP 345 EP 356 DI 10.5598/imafungus.2015.06.02.06 PG 12 WC Mycology SC Mycology GA V46UM UT WOS:000209909100026 PM 26734545 ER PT J AU Spatafora, JW Quandt, CA Kepler, RM Sung, GH Shrestha, B Hywel-Jones, NL Luangsa-ard, JJ AF Spatafora, Joseph W. Quandt, C. Alisha Kepler, Ryan M. Sung, Gi-Ho Shrestha, Bhushan Hywel-Jones, Nigel L. Luangsa-ard, J. Jennifer TI New 1F1N Species Combinations in Ophiocordycipitaceae (Hypocreales) SO IMA FUNGUS LA English DT Article DE Cordyceps; Drechmeria; Harposporium; nomenclature; Ophiocordyceps; Purpureocillium AB Based on the taxonomic and nomenclatural recommendations of Quandt et al. (2014) new species combinations are made for Ophiocordycipitaceae. These new combinations are compliant with recent changes in the International Code of Nomenclature for algae, fungi, and plants (ICN) and the abolition of the dual system of nomenclature for fungi. These changes include 10 new combinations into Drechmeria, four new combinations into Harposporium, 23 new combinations and 15 synonymies in Ophiocordyceps, and one new combination into Purpureocillium. C1 [Spatafora, Joseph W.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Quandt, C. Alisha] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Kepler, Ryan M.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Sung, Gi-Ho] Catholic Kwandong Univ, Inst Biomed Convergence & Integrat Med, Int St Marys Hosp, Incheon 404834, South Korea. [Sung, Gi-Ho] Catholic Kwandong Univ, Coll Med, Incheon 404834, South Korea. [Shrestha, Bhushan] Sungkyunkwan Univ, Inst Life Sci & Biotechnol, Suwon 440746, South Korea. [Hywel-Jones, Nigel L.] Milton Biotech Co Ltd, Khlong Luang 12120, Phathum Thani, Thailand. [Luangsa-ard, J. Jennifer] Natl Ctr Genet Engn & Biotechnol BIOTEC, Microbe Interact Lab, Khlong Luang 12120, Phathum Thani, Thailand. RP Spatafora, JW (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM joseph.spatafora@oregonstate.edu FU Cooperative Research Program for Agricultural and Technology Development of Rural Development Administration, Korea [PJ009241]; BIOTEC insect-fungus programme; National Science Foundation [DEB-1258162] FX G H Sung and B Shrestha acknowledge the financial support of the Cooperative Research Program for Agricultural and Technology Development (PJ009241) of Rural Development Administration, Korea. N L H-J would like to thank Morakot Tanticharoen for her continued support of the BIOTEC insect-fungus programme over many years. J W S acknowledges financial support (DEB-1258162) from the National Science Foundation. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. NR 15 TC 3 Z9 4 U1 0 U2 1 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD DEC PY 2015 VL 6 IS 2 BP 357 EP 362 DI 10.5598/imafungus.2015.06.02.07 PG 6 WC Mycology SC Mycology GA V46UM UT WOS:000209909100027 PM 26734546 ER PT J AU Demers, JE Romberg, MK Castlebury, LA AF Demers, Jill E. Romberg, Megan K. Castlebury, Lisa A. TI Microcyclic rusts of hollyhock (Alcea rosea) SO IMA FUNGUS LA English DT Article DE Pucciniales; Puccinia; pathogen interceptions; identification; taxonomy AB Rust fungi infecting hollyhock and other plants in Malveae are frequently intercepted at ports of entry to the USA, particularly Puccinia malvacearum and P. heterogenea. These two species can be difficult to distinguish and can be further confused with other, less common species of microcyclic rust fungi infecting hollyhock: P. heterospora, P. lobata, P. platyspora, and P. sherardiana. Molecular phylogenetic analysis revealed that P. malvacearum and P. heterogenea are closely related, along with P. sherardiana and P. platyspora. A key to the six microcyclic Puccinia species infecting hollyhock is presented. C1 [Demers, Jill E.; Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Romberg, Megan K.] USDA APHIS PPQ Natl Identificat Serv, Beltsville, MD 20705 USA. RP Demers, JE (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM Jill.Demers@ARS.USDA.GOV NR 12 TC 0 Z9 0 U1 0 U2 1 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD DEC PY 2015 VL 6 IS 2 BP 477 EP 482 DI 10.5598/imafungus.2015.06.02.11 PG 6 WC Mycology SC Mycology GA V46UM UT WOS:000209909100031 PM 26734550 ER PT J AU Gollany, HT Titus, BD Scott, DA Asbjornsen, H Resh, SC Chimner, RA Kaczmarek, DJ Leite, LFC Ferreira, ACC Rod, KA Hilbert, J Galdos, MV Cisz, ME AF Gollany, Hero T. Titus, Brian D. Scott, D. Andrew Asbjornsen, Heidi Resh, Sigrid C. Chimner, Rodney A. Kaczmarek, Donald J. Leite, Luiz F. C. Ferreira, Ana C. C. Rod, Kenton A. Hilbert, Jorge Galdos, Marcelo V. Cisz, Michelle E. TI Biogeochemical Research Priorities for Sustainable Biofuel and Bioenergy Feedstock Production in the Americas SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Agroecosystem; Bioenergy feedstock; Carbon; Forestry; Soil; Sustainability ID SOIL ORGANIC-CARBON; GREENHOUSE-GAS EMISSIONS; LONG-TERM EXPERIMENTS; CORN STOVER REMOVAL; LOBLOLLY-PINE PLANTATIONS; LIFE-CYCLE ASSESSMENT; PROCESS-BASED MODEL; MIXED OAK FOREST; LAND-USE CHANGE; ZEA-MAYS L. AB Rapid expansion in biomass production for biofuels and bioenergy in the Americas is increasing demand on the ecosystem resources required to sustain soil and site productivity. We review the current state of knowledge and highlight gaps in research on biogeochemical processes and ecosystem sustainability related to biomass production. Biomass production systems incrementally remove greater quantities of organic matter, which in turn affects soil organic matter and associated carbon and nutrient storage (and hence long-term soil productivity) and off-site impacts. While these consequences have been extensively studied for some crops and sites, the ongoing and impending impacts of biomass removal require management strategies for ensuring that soil properties and functions are sustained for all combinations of crops, soils, sites, climates, and management systems, and that impacts of biomass management (including off-site impacts) are environmentally acceptable. In a changing global environment, knowledge of cumulative impacts will also become increasingly important. Long-term experiments are essential for key crops, soils, and management systems because short-term results do not necessarily reflect long-term impacts, although improved modeling capability may help to predict these impacts. Identification and validation of soil sustainability indicators for both site prescriptions and spatial applications would better inform commercial and policy decisions. In an increasingly inter-related but constrained global context, researchers should engage across inter-disciplinary, inter-agency, and international lines to better ensure the long-term soil productivity across a range of scales, from site to landscape. C1 [Gollany, Hero T.] USDA ARS, Columbia Plateau Conservat Res Ctr, Adams, OR 97810 USA. [Titus, Brian D.] Canadian Forest Serv, Nat Resources Canada, Pacific Forestry Ctr, Victoria, BC V8Z IM5, Canada. [Scott, D. Andrew] USDA Forest Serv, Agr Res Ctr, Southern Res Stn, Normal, AL 35762 USA. [Asbjornsen, Heidi] Univ New Hampshire, Inst Earth Oceans & Space, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Asbjornsen, Heidi] Univ New Hampshire, Inst Earth Oceans & Space, Earth Syst Res Ctr, Durham, NH 03824 USA. [Resh, Sigrid C.; Chimner, Rodney A.; Cisz, Michelle E.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Kaczmarek, Donald J.] Oregon Dept Forestry, St Paul, OR 97137 USA. [Leite, Luiz F. C.] Empresa Brasileira Pesquisa Agr EMBRAPA, BR-64006220 Teresina, PI, Brazil. [Ferreira, Ana C. C.] Climate Change Adaptat Consultant, BR-71925540 Taguatinga, Brazil. [Rod, Kenton A.] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. [Hilbert, Jorge] INTA, CIA, RA-1712 Castelar, Buenos Aires, Argentina. [Galdos, Marcelo V.] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, BR-13083100 Campinas, SP, Brazil. RP Gollany, HT (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM hero.gollany@ars.usda.gov RI Galdos, Marcelo/C-3137-2012; OI Galdos, Marcelo/0000-0002-6080-0726; Scott, D. Andrew/0000-0002-2592-1522 FU U.S. National Science Foundation [CBET-1140152]; U.S. Department of Agriculture-Agricultural Research Service under the ARS-GRACEnet project; U.S. Department of Agriculture-Agricultural Research Service under the REAP project FX The authors gratefully acknowledge support in part by the U.S. National Science Foundation grant CBET-1140152 "RCN-SEES: A Research Coordination Network on Pan American Biofuels and Bioenergy Sustainability''. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. We thank reviewers for helpful comments and suggestions. This publication is based upon work supported by the U.S. Department of Agriculture-Agricultural Research Service under the ARS-GRACEnet and REAP projects. NR 283 TC 1 Z9 1 U1 3 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD DEC PY 2015 VL 56 IS 6 BP 1330 EP 1355 DI 10.1007/s00267-015-0536-7 PG 26 WC Environmental Sciences SC Environmental Sciences & Ecology GA CV0SC UT WOS:000363961900005 PM 26006220 ER PT J AU Baranowski, T AF Baranowski, Tom TI Fun and Games SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material ID ENJOYMENT; PLAY C1 Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, Pediat Behav Nutr & Phys Activ,USDA ARS, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, Pediat Behav Nutr & Phys Activ,USDA ARS, Houston, TX 77030 USA. OI Baranowski, Tom/0000-0002-0653-2222 NR 8 TC 0 Z9 0 U1 0 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD DEC 1 PY 2015 VL 4 IS 6 BP 421 EP 422 DI 10.1089/g4h.2015.0070 PG 2 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CU9TF UT WOS:000363887400001 PM 26509939 ER PT J AU Graham, ER Parekh, A Devassy, RK Sanders, RW AF Graham, Erin R. Parekh, Amy Devassy, Roni K. Sanders, Robert W. TI Carbonic anhydrase activity changes in response to increased temperature and pCO(2) in Symbiodinium-zoanthid associations SO JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY LA English DT Article DE Symbiodinium; Carbonic anhydrase; Climate change; Ocean acidification; Cnidarian; Zoanthid ID CO2 CONCENTRATING MECHANISMS; ANEMONE AIPTASIA-PULCHELLA; DISSOLVED INORGANIC CARBON; SEA-ANEMONE; SCLERACTINIAN CORAL; OCEAN ACIDIFICATION; SYNTHETIC SEAWATER; BORIC-ACID; PHOTOSYNTHESIS; CALCIFICATION AB Carbon dioxide (CO2) makes up less than 1% of dissolved inorganic carbon (DIC) in the ocean. To acquire carbon dioxide for photosynthesis, many marine autotrophs rely on the enzyme carbonic anhydrase (CA) to catalyze the conversion of bicarbonate ions, (HCO3-) to CO2. In zoanthids and other cnidarians with Symbiodinium spp. endosymbionts, CA is essential for transporting CO2 to symbionts for photosynthesis. Temperature and ambient DIC affect CA activity, therefore, increased sea water temperatures and ocean acidification (OA) will alter CO2 transport in symbiotic cnidarians. However, these effects are likely to be species specific for both host and symbiont, as different cnidarians and Symbiodinium spp. vary in their mechanisms of DIC transport and utilization of CA. In this study, host and symbiont CA activity in the zoanthids Palythoa sp. and Zoanthus sp. varied with thermal stress and low pH. Increased temperature inhibited algal, but not host CA activity in Zoanthus sp. polyps with A4 Symbiodinium, while temperature had no effect on CA activity in Palythoa sp. with Cl Symbiodinium. High pCO(2)/low pH altered algal CA activity in both zoanthid species, but host CA activity changed in Zoanthus sp. polyps only. This study shows that thermal stress and OA induce species-specific changes in CA activity, and thus DIC transport in symbiotic zoanthids. These observations suggest that CA activity in symbiotic cnidarians will be altered by climate conditions predicted for the future, and for some cnidarians, changes in CA activity may inhibit photosynthesis. (C) 2015 Elsevier B.V. All rights reserved. C1 [Graham, Erin R.; Parekh, Amy; Devassy, Roni K.; Sanders, Robert W.] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA. [Graham, Erin R.] USDA ARS, Hatfield Marine Sci Ctr, Newport, OR USA. RP Graham, ER (reprint author), USDA ARS, Hatfield Marine Sci Ctr, 2030 SE Marine Sci Dr, Newport, OR USA. EM erin.graham@oregonstate.edu RI Sanders, Robert/C-1116-2011 OI Sanders, Robert/0000-0001-7264-1059 FU National Science Foundation [0838847] FX The authors wish to thank T. LaJeunesse, A. Lewis and J. Reimer for their help with symbiont and host species identification and J. Lunden for his assistance in manipulating carbonate chemistry. Zoanthid collection was permitted under the NOAA Office of National Marine Sanctuaries (no. FKNMS-2009-093). This work was partially supported by the National Science Foundation grant 0838847 to RWS. [SS] NR 52 TC 1 Z9 1 U1 10 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-0981 EI 1879-1697 J9 J EXP MAR BIOL ECOL JI J. Exp. Mar. Biol. Ecol. PD DEC PY 2015 VL 473 BP 218 EP 226 DI 10.1016/j.jembe.2015.08.017 PG 9 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CV4TI UT WOS:000364259000026 ER PT J AU Haye, T Gariepy, T Hoelmer, K Rossi, JP Streito, JC Tassus, X Desneux, N AF Haye, Tim Gariepy, Tara Hoelmer, Kim Rossi, Jean-Pierre Streito, Jean-Claude Tassus, Xavier Desneux, Nicolas TI Range expansion of the invasive brown marmorated stinkbug, Halyomorpha halys: an increasing threat to field, fruit and vegetable crops worldwide SO JOURNAL OF PEST SCIENCE LA English DT Review DE Agricultural pest; Invasion scenarios; Distribution; Europe; Asia; North America ID BUG HEMIPTERA-PENTATOMIDAE; HETEROPTERA-PENTATOMIDAE; GENETIC DIVERSITY; STAL; PEST; ONTARIO; CANADA; INJURY; EUROPE; APPLE AB The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), has emerged as a harmful invasive insect pest in North America and Europe in the 1990s and 2000s, respectively. Native to eastern Asia, this highly polyphagous pest (> 120 different host plants) is spreading rapidly worldwide, notably through human activities. The increasing global importance of the pest suggests that more coordinated actions are needed to slow its spread and mitigate negative effects in invaded areas. Prevention of large-scale outbreaks will require accurate identification and effective mitigation tools to be rapidly developed and widely implemented. In this short review, we update the current distribution of H. halys, discuss potential geographic range expansion based on passive and active dispersal and provide insight on the economic, environmental and social impact associated with H. halys. C1 [Haye, Tim] CABI, CH-2800 Delemont, Switzerland. [Gariepy, Tara] Agr & Agri Food Canada, Southern Crop Protect & Food Res Ctr, London, ON N5V 4T3, Canada. [Hoelmer, Kim] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Rossi, Jean-Pierre; Streito, Jean-Claude] INRA, CBGP, F-34988 Montferrier Sur Lez, France. [Tassus, Xavier] ANSES French Agcy Food Environm & Occupat Hlth &, Plant Hlth Lab, Expertise & Biol Risk Unit, F-49044 Angers 01, France. [Desneux, Nicolas] INRA, French Natl Inst Agr Res, F-06903 Sophia Antipolis, France. [Haye, Tim] Chinese Acad Agr Sci, Inst Plant Protect, MoA CABI Joint Lab Biosafety, Beijing 100193, Peoples R China. RP Haye, T (reprint author), CABI, Rue Grillons 1, CH-2800 Delemont, Switzerland. EM t.haye@cabi.org OI Tassus, Xavier/0000-0002-2796-7081 FU French Agency for Food, Environmental and Occupational Health Safety (ANSES) FX We would like to thank the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) for supporting this review. NR 63 TC 12 Z9 13 U1 16 U2 95 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD DEC PY 2015 VL 88 IS 4 BP 665 EP 673 DI 10.1007/s10340-015-0670-2 PG 9 WC Entomology SC Entomology GA CV0ZI UT WOS:000363982200001 ER PT J AU Tooley, PW Browning, M AF Tooley, Paul W. Browning, Marsha TI Temperature Effects on the Onset of Sporulation by Phytophthora ramorum on Rhododendron "Cunningham's White' SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE sudden oak death; sporangia ID POTATO LATE BLIGHT; LEAF WETNESS; IN-VITRO; INFECTION; SUSCEPTIBILITY; PATHOGEN; FOREST; PERIOD; HOST; SIMULATION AB The effect of temperature and moist period on the onset of sporangia production by Phytophthora ramorum on Rhododendron Cunningham's White' was examined with misted detached leaves held in humid chambers. Following wound inoculation with sporangia, leaves were pre-incubated at 20 degrees C for either 24 or 72h prior to placement at six different temperatures (4, 10, 15, 20, 25 and 30 degrees C). The overall mean moist period required for first occurrence of sporulation over all six temperatures was 3.24days with the 24-h pre-incubation time, compared with 1.49days for the 72-h pre-incubation time. Following 24h pre-incubation at 20 degrees C and at an incubation temperature of 15 degrees C, sporangia were first collected from leaves following a 24h incubation. At 10 and 20 degrees C, sporangia were first collected after 48h, whereas at 4, 25 and 30 degrees C, sporangia were first collected after 3days. Following 72h pre-incubation at 20 degrees C, sporulation generally occurred within 1day, even at temperatures such at 4 and 30 degrees C that are suboptimal for sporulation. The highest levels of P.ramorum sporulation were observed at 20 degrees C. P.ramorum formed sporangia on host tissue under moist conditions within the same time frame reported for P.phaseoli, P.palmivora and P.nicotianae, but substantially more slowly than certain other species such as P.infestans. Quantifying moisture and temperature conditions for initiation of sporangia production provides knowledge which leads to a greater understanding of the epidemic potential of P.ramorum. C1 [Tooley, Paul W.; Browning, Marsha] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Tooley, PW (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM paul.tooley@ars.usda.gov NR 44 TC 0 Z9 0 U1 5 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD DEC PY 2015 VL 163 IS 11-12 BP 908 EP 914 DI 10.1111/jph.12390 PG 7 WC Plant Sciences SC Plant Sciences GA CU7OE UT WOS:000363730100004 ER PT J AU Wang, MQ Li, F Zhou, GH Lan, PX Xu, DL Li, RH AF Wang, Mingqiang Li, Fan Zhou, Guohui Lan, Pingxiu Xu, Donglin Li, Ruhui TI Molecular Detection and Characterization of Chinese Yam Mild Mosaic Virus Isolates SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE genetic divergence; genomic sequence; RT-PCR detection; Yam mild mosaic virus ID COMPLETE NUCLEOTIDE-SEQUENCE; GENOMIC RNA; WATER YAM; POTYVIRUSES; NIGERIA; PCR AB An improved RT-PCR was developed and validated for the detection of Yam mild mosaic virus (YMMV). Sequences of the coat protein core region of 19 Chinese isolates were obtained, and analysis indicated the presence of different genetic variants. Phylogenetic analyses showed that the Chinese isolates were divided into two distinct clusters. Complete genomic sequences of two distinct Chinese variants were determined to be 9527 and 9529 nucleotides long, excluding the 3 poly (A) tail. Their genomic structure and organization were virtually identical to that of a Brazilian isolate. The two variants shared identity of 87.3% to one another and 83.9-84.6% to the Brazilian variant at the genomic sequence level. Phylogenetic analyses supported that they represented two distinct YMMV lineages. C1 [Wang, Mingqiang; Lan, Pingxiu; Xu, Donglin; Li, Ruhui] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Wang, Mingqiang; Zhou, Guohui; Xu, Donglin] South China Agr Univ, Lab Plant Virol, Guangzhou 510642, Guangdong, Peoples R China. [Li, Fan; Lan, Pingxiu] Yunnan Agr Univ, China Educ Minist, Key Lab Agr Biodivers Pest Management, Kunming 650201, Peoples R China. RP Li, RH (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. EM Ruhui.Li@ars.usda.gov OI Xu, Dongin/0000-0002-5719-2950 NR 22 TC 0 Z9 0 U1 2 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD DEC PY 2015 VL 163 IS 11-12 BP 1036 EP 1040 DI 10.1111/jph.12337 PG 5 WC Plant Sciences SC Plant Sciences GA CU7OE UT WOS:000363730100021 ER PT J AU Gordon, SH Mohamed, AA Harry-Okuru, RE Biresaw, G AF Gordon, S. H. Mohamed, A. A. Harry-Okuru, R. E. Biresaw, G. TI Identification and Measurement of Intermolecular Interaction in Polyester/Polystyrene Blends by FTIR-Photoacoustic Spectrometry SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Biodegradable plastic; Polyester/polystyrene blends; Intermolecular interaction; n-pi interaction; Infrared spectral deconvolution ID TRANSFORM INFRARED-SPECTROSCOPY; POLYMER BLENDS; INTERFACIAL-TENSION; COMPATIBILITY; POLYSTYRENE; GENTAMICIN; PROTEIN; SAMPLES; ACID) AB Fourier transform infrared photoacoustic spectrometry was used to reveal and identify n-pi type intermolecular interaction formed in plastic comprising binary blends of polystyrene and a biodegradable polymer, either polylactic acid, polycaprolactone or poly(tetramethyleneadipate-co-terephthalate). This was the first reported direct and definitive experimental evidence of n-pi type bond formation in thermoplastic blends of these polyesters with polystyrene. Also, an infrared method was devised that permits quantitative measurement of the degree of interaction between the two polymers at various concentrations in the blends. The method employs spectral deconvolution by least squares curve fitting of the polymer carbonyl band into its underlying peaks. In a new algorithm the method compares deconvoluted Gaussian/Lorentzian peaks of the polymer blends with deconvoluted peaks in the neat polymers and computes both the magnitude and direction of change in the n-pi bond formation with change in polymer concentration. The results indicated that the degree of interaction was dependent on the type and concentration of the biodegradable polymer in the blend. These findings are supported by differential scanning calorimetry and thermogravimetric analyses. Unlike conventional spectral deconvolution methods, this technique with its new algorithm approximates infrared absorptivities of all the underlying peaks, and is thus a superior method that should be applicable to multicomponent polymer blends in general. C1 [Gordon, S. H.] ARS, Plant Polymer Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Mohamed, A. A.] King Saud Univ, Dept Food Sci & Nutr, Riyadh 11451, Saudi Arabia. [Harry-Okuru, R. E.; Biresaw, G.] ARS, Biooils Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Gordon, SH (reprint author), ARS, Plant Polymer Res, Natl Ctr Agr Utilizat Res, USDA, 1815N Univ St, Peoria, IL 61604 USA. EM sherald.gordon@ars.usda.gov NR 40 TC 0 Z9 0 U1 4 U2 16 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD DEC PY 2015 VL 23 IS 4 BP 459 EP 469 DI 10.1007/s10924-015-0731-x PG 11 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA CV1AA UT WOS:000363984000004 ER PT J AU Jong, L AF Jong, Lei TI Toughness of Natural Rubber Composites Reinforced with Hydrolyzed and Modified Wheat Gluten Aggregates SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Mechanical properties; Natural rubber; Rubber filler; Wheat gluten ID VISCOELASTIC PROPERTIES; MECHANICAL-PROPERTIES; FILLER INTERACTIONS; STARCH; NANOCRYSTALS; ENHANCEMENT; PROTEIN AB The toughness of natural rubber can be improved by using fillers for various rubber applications. Dry wheat gluten is a protein from wheat flour and is sufficiently rigid for rubber reinforcement. The wheat gluten was hydrolyzed to reduce its particle size and microfluidized to reduce and homogenize the particle size and size distribution. The processed wheat gluten aggregates with a size of similar to 500 nm were used to improve the toughness of natural rubber. The crosslinked rubber composites showed an improvement in moduli, tensile strength and toughness compared to natural rubber; had a greater toughness and elongation than the carbon black filled composites at higher filler fractions. For the composites prepared under the alkali condition, the modified particles increased the moduli in the larger strain region. For the composites prepared under the acidic condition, the modified particles improved the tensile strength, elongation, and toughness. The reinforced composites were characterized with stress-strain, dynamic frequency sweep, and swelling experiments to understand their structure-properties. C1 USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, Peoria, IL 61604 USA. RP Jong, L (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, 1815 N Univ St, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 27 TC 1 Z9 1 U1 1 U2 4 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD DEC PY 2015 VL 23 IS 4 BP 541 EP 550 DI 10.1007/s10924-015-0728-5 PG 10 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA CV1AA UT WOS:000363984000013 ER PT J AU Wang, WX Sabo, RC Mozuch, MD Kersten, P Zhu, JY Jin, YC AF Wang, Wangxia Sabo, Ronald C. Mozuch, Michael D. Kersten, Phil Zhu, J. Y. Jin, Yongcan TI Physical and Mechanical Properties of Cellulose Nanofibril Films from Bleached Eucalyptus Pulp by Endoglucanase Treatment and Microfluidization SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Endoglucanase treatment; Microfluidization; Cellulose nanofibrils (CNF); CNF films; Eucalyptus pulp ID ENERGY-CONSUMPTION; HYDROLYSIS; NANOPAPER; FIBER AB A GH5 hyperthermostable endoglucanase (Ph-GH5) from the archaeon Pyrococcus horikoshii and a commercial endoglucanase (FR) were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNF) and subsequently to CNF films. TEM imaging indicated that Ph-GH5 produced longer and more entangled CNF than FR with the same number of microfluidization passes. Physical and mechanical properties of CNF films were characterized. Optical opacity of CNF films from FR (10 mg/g) at 40 passes through the microfluidizer can be as low as 3.7 %, compared with 18.2 % from untreated BEP at the same number of passes. CNF films exhibited similar thermal stability with untreated BEP. Highest specific modulus of CNF films was also obtained from FR (10 mg/g), reaching 56 MNm/kg, approximately 271 % of the CNF films from untreated BEP at 40 passes through the microfluidizer. CNF film from Ph-GH5 (1 mg/g) at 40 passes provided the highest specific maximum tensile strength at 120 kNm/kg. C1 [Wang, Wangxia; Jin, Yongcan] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China. [Wang, Wangxia; Sabo, Ronald C.; Mozuch, Michael D.; Kersten, Phil; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Kersten, P (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM pkersten@fs.fed.us; jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; Chinese Scholarship Council (CSC); National Natural Science Foundation of China [31070512, 31370571] FX We acknowledge the financial supports by a USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), National Natural Science Foundation of China (Grant Nos. 31070512 and 31370571). The funding from these programs made the visiting appointment of Wang at the USDA Forest Products Laboratory (FPL) possible. We also acknowledge Thomas Kuster of the Analytical Chemistry and Microscopy Lab of FPL for SEM imaging and Debra Sherman of DS imaging LLC, West Lafayette, IN, for TEM imaging. This work was conducted on official government time of Zhu, Kersten, Mozuch, and Sabo while Wang was a visiting student at the US Forest Service, Forest Products Lab. NR 24 TC 6 Z9 6 U1 6 U2 25 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD DEC PY 2015 VL 23 IS 4 BP 551 EP 558 DI 10.1007/s10924-015-0726-7 PG 8 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA CV1AA UT WOS:000363984000014 ER PT J AU Tworkoski, T Webb, K Callahan, A AF Tworkoski, Thomas Webb, Kevin Callahan, Ann TI Auxin levels and MAX1-4 and TAC1 gene expression in different growth habits of peach SO PLANT GROWTH REGULATION LA English DT Article DE Auxin; Cytokinin; Gene expression; Hormone; Indole-3-acetic acid; Strigolactone; Tree architecture ID TANDEM MASS-SPECTROMETRY; APPLE-TREES; APICAL DOMINANCE; HORMONAL-CONTROL; PLANT HORMONES; BUD OUTGROWTH; ABSCISIC-ACID; SHOOT GROWTH; GRAVITROPISM; CYTOKININ AB Branch orientation and distribution determine tree architecture that can influence orchard design and management. Peach [Prunus persica L. (Batch)] trees with three different branching genotypes were evaluated: fewer, nearly vertical branches (pillar), less vertical and more spreading branches (upright), and more abundant, least vertical branches (standard). Auxin concentrations and expression of genes that regulate branch development in herbaceous species, MAX1, 2, 3, 4 and TAC1 were determined. Shoots and roots of peach trees in the field and greenhouse were studied following pruning and during periods of growth when bud break and branch spatial orientation develop. Expression of MAX3 and MAX4 decreased in stems of field-grown peach trees that remained on the tree following pruning. In the greenhouse elevated auxin concentrations and higher gene expression of MAX3 in roots and MAX4 in stems were found in pillar rather than standard trees. Upright trees had auxin and MAX1-4 expression that was intermediate between pillar and standard trees. Temporal differences were found with MAX1-4 expression being greater in April or May but auxin concentrations were greater only in shoots in May. Expression of TAC1 was inversely related with auxin concentrations in shoots and was greatest in standard and least in pillar trees. The current work indicates that in stems, auxin, MAX3-4 genes, and TAC1 genes may influence regulatory processes that affect growth and development of peach trees with different growth habits. In addition to breeding, new plant growth regulators that affect the modes of action of root-originating signals may provide new cultural tools for managing tree growth and development. C1 [Tworkoski, Thomas; Webb, Kevin; Callahan, Ann] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Tworkoski, T (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM tom.tworkoski@ars.usda.gov NR 47 TC 1 Z9 1 U1 9 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6903 EI 1573-5087 J9 PLANT GROWTH REGUL JI Plant Growth Regul. PD DEC PY 2015 VL 77 IS 3 BP 279 EP 288 DI 10.1007/s10725-015-0062-x PG 10 WC Plant Sciences SC Plant Sciences GA CV1MW UT WOS:000364023400002 ER PT J AU Jirsa, D Barrows, FT Hardy, RW Drawbridge, M AF Jirsa, D. Barrows, F. T. Hardy, R. W. Drawbridge, M. TI Alternative protein blends as a replacement for fish meal in diets for white seabass, Atractoscion nobilis SO AQUACULTURE NUTRITION LA English DT Article DE alternative proteins; atractoscion nobilis; diet; finfish; fish meal replacement; nutrition ID TROUT ONCORHYNCHUS-MYKISS; DRUM SCIAENOPS-OCELLATUS; SALMON SALMO-SALAR; BY-PRODUCT MEALS; RAINBOW-TROUT; SOYBEAN-MEAL; APPARENT DIGESTIBILITY; RED DRUM; ATLANTIC SALMON; PRACTICAL DIETS AB An 8-week feeding trial was conducted to determine the effectiveness of replacing fish meal (FM) with blends of alternative proteins in diets for white seabass (WSB, Atractoscion nobilis) at a starting weight of 5.6g. Five diets were formulated with 400-440gkg(-1) crude protein (380gkg(-1) digestible). These included a high 520gkg(-1) FM control diet, a series of three diets with a sequential replacement of FM containing 410gkg(-1), 510gkg(-1) and 630gkg(-1) of a soy-based protein blend (SPC) and 200gkg(-1), 100gkg(-1) and 0gkg(-1) FM, respectively and a fifth diet containing 550gkg(-1) of a corn-based protein blend (CGM) and 100gkg(-1) FM. Survival was highest in the FM control group at 99% but all other performance measures (weight gain, feed conversion ratio, specific growth rate and protein retention efficiency) were worse than the other treatment groups. Weight gain reached a maximum of 595% in the SPC 200gkg(-1) FM treatment group. Performance decreased as inclusion of the soy-based protein blend increased. The CGM treatment performed comparably to the SPC 100g kg(-1) FM treatment among all measures, except for survival, which was higher in the CGM 100gkg(-1) FM treatment. With nutrient levels and alternative protein blends used in this study, FM can be reduced to 100gkg(-1) of the diet for WSB without reductions in performance. C1 [Jirsa, D.; Drawbridge, M.] Hubbs SeaWorld Res Inst, San Diego, CA 92109 USA. [Barrows, F. T.] ARS, USDA, Bozeman, MT USA. [Hardy, R. W.] Univ Idaho, Inst Aquaculture Res, Hagerman, ID USA. RP Jirsa, D (reprint author), Hubbs SeaWorld Res Inst, 2595 Ingraham St, San Diego, CA 92109 USA. EM djirsa@hswri.org FU California Sea Grant [R/AQ-130]; National Oceanographic and Atmospheric Association [NA08OAR4170669]; California's Ocean Resources Enhancement and Hatchery Programme FX The authors would like to thank Andrea Marino, Jose Velazquez, Karla Aguero and the staff at HSWRI's marine fish hatchery and Jason Frost at United States Department of Agriculture - Agriculture Research Service for feed production. This research was funded by California Sea Grant project number R/AQ-130, National Oceanographic and Atmospheric Association grant number NA08OAR4170669, with support from California's Ocean Resources Enhancement and Hatchery Programme. NR 53 TC 1 Z9 1 U1 1 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD DEC PY 2015 VL 21 IS 6 BP 861 EP 867 DI 10.1111/anu.12212 PG 7 WC Fisheries SC Fisheries GA CU8BM UT WOS:000363766100011 ER PT J AU Trotter, RT Hull-Sanders, HM AF Trotter, R. Talbot, III Hull-Sanders, Helen M. TI Quantifying Dispersal of the Asian longhorned beetle (Anoplophora glabripennis, Coleoptera) with incomplete data and behavioral knowledge SO BIOLOGICAL INVASIONS LA English DT Article DE Invasion dynamics; Population spread; Dispersal kernel; Host adjacency ID UNITED-STATES; ECONOMIC COSTS; CERAMBYCIDAE; ERADICATION; MANAGEMENT; INVASIONS; SPREAD AB Eradication programs for invasive species can benefit from tools that delineate infestations and identify patterns of spread to guide eradication priorities and activities. However, identifying these patterns in cryptic organisms such the Asian longhorned beetle can be complicated by the sometimes conflicting needs of rapid eradication and research. Here, we describe the use of a simple approach based on tools and concepts used in graph theory to infer beetle movement, using infested tree records collected by the Asian Longhorned Beetle Eradication Program in Worcester, MA, the largest infestation yet found in the U.S. Analyses included two sets of assumptions about beetle dispersal (representing a gap in knowledge of beetle biology), and two data sets of varying completeness, which were combined to develop and compare four scenarios of beetle dispersal in Worcester, MA. Together, these four scenarios suggest that the shape of the beetle dispersal-distance probability curve or dispersal kernel is more sensitive to assumptions about the predilection of beetles to disperse than to the size and completeness of the infested tree database, though both impacted inferred patterns of dispersal. The four scenarios are used to produce empirical estimates of dispersal risk around the current infestation, which can inform eradication efforts while recognizing the limits of data availability in a rapidly evolving eradication program. These estimates of dispersal also highlight the importance of continuing to integrate data collection into eradication programs, and the need to expand our understanding of beetle behavior and biology, as the data shown suggest that differences in dispersal behavior could dictate different eradication strategies. C1 [Trotter, R. Talbot, III] USDA, Forest Serv, No Res Stn, Hamden, CT 06514 USA. [Hull-Sanders, Helen M.] USDA, Anim & Plant Hlth Inspect Serv, Ctr Plant Hlth Sci & Technol, Otis Lab, Buzzards Bay, MA USA. RP Trotter, RT (reprint author), USDA, Forest Serv, No Res Stn, 51 Mill Pond Rd, Hamden, CT 06514 USA. EM rttrotter@fs.fed.us; hhullsanders@gmail.com FU USDA Forest Service, Northern Research Station; USDA APHIS Center for Plant Health Science and Technology FX We thank Clint McFarland, Ryan Vazquez, and Eugene Pepper with the United States Department of Agriculture Animal and Plant Health Inspection Service Plant Pest Quarantine Asian Longhorned Beetle Eradication Program and Audra Baker and William Panagakos with the United States Department of Agriculture Animal and Plant Health Inspection Service Center for Plant Health Science and Technology for their help in accessing and interpreting the infested tree databases, and we thank the numerous field surveyors who have surveyed the 5 million-plus trees in the regulated areas. We also thank three anonymous reviewers for their comments, which greatly improved the clarity and structure of the paper. Support for this work was provided by the USDA Forest Service, Northern Research Station, and the USDA APHIS Center for Plant Health Science and Technology. NR 36 TC 0 Z9 0 U1 4 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD DEC PY 2015 VL 17 IS 12 BP 3359 EP 3369 DI 10.1007/s10530-015-0961-9 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CU4FM UT WOS:000363482600002 ER PT J AU Hogg, BN Daane, KM AF Hogg, Brian N. Daane, Kent M. TI Cascading effects of cannibalism in a top predator SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Araneae; cannibalism; Cheiracanthium; herbivore suppression; intra-guild predation; predator diversity; predator interactions; size structure ID INTRAGUILD PREDATION; STRUCTURED POPULATIONS; BODY-SIZE; INTERMEDIATE PREDATORS; TROPHIC INTERACTIONS; EMERGENT IMPACTS; INVASION SUCCESS; NICHE OVERLAP; PREY SYSTEMS; WOLF SPIDERS AB 1. Predator interactions are often driven by size differences, with larger predators consuming smaller ones. Cannibalism is widespread among predators, and can be more intense than antagonistic interactions between different predator species. 2. In a series of greenhouse experiments and a field experiment, we investigated the role of size structure in shaping the impacts of a top predator (the wandering spider Cheiracanthium mildeiL. Koch) on an insect herbivore in vineyards of Napa County, California. 3. In greenhouse cages, survival of small C. mildei was lower when large C. mildei were present, and the combined effects of small and large C. mildei on leafhopper numbers and grape leaf damage were non-additive. The frequency of cannibalism in C. mildei did not change when the abundance of leafhoppers was manipulated. However, in the field experiment small and large C. mildei combined additively to reduce leafhopper numbers. 4. Results show that cannibalism has the potential to dampen the cascading effects of C. mildei in a vineyard food web, but that it may be less important than other mortality factors in the field. Nonetheless, C. mildei substantially impacted leafhoppers in both the greenhouse and the field, suggesting that mortality caused by cannibalism or other factors may not compromise its overall predatory impacts. C1 [Hogg, Brian N.; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Hogg, BN (reprint author), USDA ARS, 800 Buchanan St, Albany, CA 94710 USA. EM hoggbrian@yahoo.com FU American Vineyard Foundation FX We wish to thank the American Vineyard Foundation for funding, vineyard managers for use of their farm, and John Hutchins, Brandy Chavez, Rosemarie Radford, and Sharon Lebenkoff for help in the laboratory and field. We are also grateful for the insightful comments of the associate editor and two anonymous referees. B.N.H. designed and conducted the experiments and analysed the data input from K.M.D. B.N.H. and K.M.D. wrote the manuscript. NR 43 TC 0 Z9 0 U1 5 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD DEC PY 2015 VL 40 IS 6 BP 805 EP 813 DI 10.1111/een.12273 PG 9 WC Entomology SC Entomology GA CU8MU UT WOS:000363797900018 ER PT J AU Acheson, RJ Woerner, DR Martin, JN Belk, KE Engle, TE Brown, TR Brooks, JC Luna, AM Thompson, LD Grimes, HL Arnold, AN Savell, JW Gehring, KB Douglass, LW Howe, JC Patterson, KY Roseland, JM Williams, JR Cifelli, A Leheska, JM McNeill, SH AF Acheson, R. J. Woerner, D. R. Martin, J. N. Belk, K. E. Engle, T. E. Brown, T. R. Brooks, J. C. Luna, A. M. Thompson, L. D. Grimes, H. L. Arnold, A. N. Savell, J. W. Gehring, K. B. Douglass, L. W. Howe, J. C. Patterson, K. Y. Roseland, J. M. Williams, J. R. Cifelli, A. Leheska, J. M. McNeill, S. H. TI Nutrient database improvement project: Separable components and proximate composition of raw and cooked retail cuts from the beef loin and round SO MEAT SCIENCE LA English DT Article DE Beef; Composition; Loin; Nutrition; Retail; Round ID USDA QUALITY GRADE; EXTERNAL FAT; YIELD GRADE; CHOLESTEROL CONTENT; MEAT CONSUMPTION; HEART-DISEASE; CARCASS; MUSCLES; STEAKS; STEERS AB Beef nutrition research has become increasingly important domestically and internationally for the beef industry and its consumers. The objective of this study was to analyze the nutrient composition of ten beef loin and round cuts to update the nutrient data in the USDA National Nutrient Database for Standard Reference. Seventy-two carcasses representing a national composite of Yield Grade, Quality Grade, sex classification, and genetic type were identified from six regions across the U.S. Beef short loins, strip loins, tenderloins, inside rounds, and eye of rounds (NAMP # 173, 175, 190A, 169A, and 171C) were collected from the selected carcasses and shipped to three university meat laboratories for storage, retail fabrication, and raw/cooked analysis of nutrients. Sample homogenates from each animal were analyzed for proximate composition. These data provide updated information regarding the nutrient status of beef, in addition, to determining the influence of Quality Grade, Yield Grade, and sex classification on nutrient composition. (C) 2015 Published by Elsevier Ltd. C1 [Acheson, R. J.; Woerner, D. R.; Martin, J. N.; Belk, K. E.; Engle, T. E.] Colorado State Univ, Dept Anim Sci, Ctr Meat Safety & Qual, Ft Collins, CO 80523 USA. [Brown, T. R.; Brooks, J. C.; Thompson, L. D.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Grimes, H. L.; Arnold, A. N.; Savell, J. W.; Gehring, K. B.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Howe, J. C.; Patterson, K. Y.; Roseland, J. M.; Williams, J. R.] USDA, Beltsville, MD 20705 USA. [Cifelli, A.; McNeill, S. H.] Natl Cattlemens Beef Assoc, Centennial, CO 80112 USA. [Luna, A. M.] Land OLakes Purina Mills, Lubbock, TX 79403 USA. RP Woerner, DR (reprint author), Colorado State Univ, Dept Anim Sci, Campus Delivery 1170, Ft Collins, CO 80523 USA. EM dale.woerner@colostae.edu RI Savell, Jeffrey/C-2434-2008 OI Savell, Jeffrey/0000-0003-0354-1286 NR 33 TC 3 Z9 3 U1 3 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD DEC PY 2015 VL 110 BP 236 EP 244 DI 10.1016/j.meatsci.2015.06.001 PG 9 WC Food Science & Technology SC Food Science & Technology GA CU8XP UT WOS:000363827700032 PM 26280470 ER PT J AU Liang, GH Jang, EB Heller, WP Chang, CL Chen, JH Zhang, FP Geib, SM AF Liang, Guang Hong Jang, Eric B. Heller, Wade P. Chang, Chiou Ling Chen, Jia Hua Zhang, Fei Ping Geib, Scott M. TI A qPCR-based method for detecting parasitism of Fopius arisanus (Sonan) in oriental fruit flies, Bactrocera dorsalis (Hendel) SO PEST MANAGEMENT SCIENCE LA English DT Article DE qPCR; parasitism rate; Fopius arisanus (Sonan); Bactrocera dorsalis (Hendel) ID COMPLEX HYMENOPTERA-BRACONIDAE; COPTERA-HAYWARDI HYMENOPTERA; DIPTERA TEPHRITIDAE; BIOLOGICAL-CONTROL; MOLECULAR MARKERS; OLIVE FLY; ANASTREPHA-FRATERCULUS; PSYTTALIA-CONCOLOR; CEREAL APHIDS; NORTH-AMERICA AB BACKGROUNDParasitism detection and species identification are necessary in fruit fly biological control. Currently, release of mass-reared Fopius arisanus is practiced worldwide, as it is effective in controlling Bactrocera dorsalis and Ceratitis capitata. To detect and assess parasitism in parasitoid mass-rearing colonies and parasitism levels in field populations across all life stages of hosts, the development of a rapid, specific and sensitive method is important. RESULTSA species-specific probe was designed for F. arisanus, as well as a universal tephritid probe. Utilizing rapid DNA extraction techniques coupled with quantitative-PCR, a simple and fast assay has been developed to detect parasitism of F. arisanus that is sensitive enough to detect the parasitoid across all developmental stages, including a single egg per host egg or 0.25ng of parasitoid DNA in 40ng of host DNA. The qPCR methods also detect a higher parasitism rate when compared with rearing-based methods where parasitism rate is based on wasp emergence and where unemerged wasps are not included. CONCLUSIONThis method is a rapid, sensitive and specific technique to determine the parasitism rate of F. arisanus across all life stages of B. dorsalis, which will be useful to predict parasitoid output from mass rearing and evaluate the outcome of pest suppression after mass release in the field. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Liang, Guang Hong; Zhang, Fei Ping] Fujian Agr & Forestry Univ, Coll Forestry, Fuzhou, Peoples R China. [Jang, Eric B.; Heller, Wade P.; Chang, Chiou Ling; Geib, Scott M.] USDA ARS, DKI PBARC, Hilo, HI 96720 USA. [Chen, Jia Hua] Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou, Peoples R China. RP Geib, SM (reprint author), USDA ARS, DKI PBARC, 64 Nowelo St, Hilo, HI 96720 USA. EM scott.geib@ars.usda.gov OI Heller, Wade/0000-0002-5964-9715; Geib, Scott/0000-0002-9511-5139 FU China Scholarship Council; Funding of State Forestry Administration of China [201304401]; USDA-ARS Daniel K Inouye US Pacific Basin Agricultural Research Center in Hilo, Hawaii FX This study was financially supported by the China Scholarship Council, the Funding of State Forestry Administration of China (201304401) and the USDA-ARS Daniel K Inouye US Pacific Basin Agricultural Research Center in Hilo, Hawaii. We are grateful to Michael P McKenney and Keith Shigetani for providing lab strains of parasitoids and fruit flies, and to Thomas E Mangine for providing wild strains. We also thank Nicholas Manoukis, Steven Tam, Sheina Sim, Bernarda Calla, Yang Yu and Mathew Siderhurst for technical and statistical support. USDA is an equal opportunity provider and employer. NR 53 TC 0 Z9 0 U1 3 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD DEC PY 2015 VL 71 IS 12 BP 1666 EP 1674 DI 10.1002/ps.3976 PG 9 WC Agronomy; Entomology SC Agriculture; Entomology GA CU7BH UT WOS:000363689400012 PM 25641860 ER PT J AU Miller, RS Sweeney, SJ Akkina, JE Saito, EK AF Miller, R. S. Sweeney, S. J. Akkina, J. E. Saito, E. K. TI Potential Intercontinental Movement of Influenza A(H7N9) Virus into North America by Wild Birds: Application of a Rapid Assessment Framework SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE H7N9; wild bird; movement; intercontinental; avian influenza; qualitative; assessment; pathway; emerging diseases; zoonosis ID MALLARDS ANAS-PLATYRHYNCHOS; GENE SEGMENT REASSORTMENT; AVIAN INFLUENZA; A VIRUSES; MIGRATORY BIRDS; EXPERIMENTAL-INFECTION; H5N1 VIRUS; HPAI H5N1; DUCKS; WATERFOWL AB A critical question surrounding emergence of novel strains of avian influenza viruses (AIV) is the ability for wild migratory birds to translocate a complete (unreassorted whole genome) AIV intercontinentally. Virus translocation via migratory birds is suspected in outbreaks of highly pathogenic strain A(H5N1) in Asia, Africa and Europe. As a result, the potential intercontinental translocation of newly emerging AIV such as A(H7N9) from Eurasia to North America via migratory movements of birds remains a concern. An estimated 2.91 million aquatic birds move annually between Eurasia and North America with an estimated AIV prevalence as high as 32.2%. Here, we present a rapid assessment to address the likelihood of whole (unreassorted)-genome translocation of Eurasian strain AIV into North America. The scope of this assessment was limited specifically to assess the weight of evidence to support the movement of an unreassorted AIV intercontinentally by migratory aquatic birds. We developed a rapid assessment framework to assess the potential for intercontinental movement of avian influenzas by aquatic birds. This framework was iteratively reviewed by a multidisciplinary panel of scientific experts until a consensus was established. Our assessment framework identified four factors that may contribute to the potential for introduction of any AIV intercontinentally into North America by wild aquatic birds. These factors, in aggregate, provide a framework for evaluating the likelihood of new forms of AIV from Eurasia to be introduced by aquatic birds into North America. Based on our assessment, we determined that the potential for introduction of A(H7N9) into North America through aquatic migratory birds is possible, but the likelihood ranges from extremely low to low. C1 [Miller, R. S.; Sweeney, S. J.; Akkina, J. E.; Saito, E. K.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. RP Miller, RS (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Epidemiol & Anim Hlth, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Ryan.Miller@rsmiller.net OI Miller, Ryan/0000-0003-3892-0251 NR 111 TC 3 Z9 3 U1 5 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD DEC PY 2015 VL 62 IS 6 BP 650 EP 668 DI 10.1111/tbed.12213 PG 19 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA CU7NF UT WOS:000363726300011 PM 24589158 ER PT J AU Velasquez, A Pohlenz, C Barrows, FT Gaylord, TG Gatlin, DM AF Velasquez, Alejandro Pohlenz, Camilo Barrows, Frederic T. Gaylord, T. Gibson Gatlin, Delbert M., III TI Assessment of taurine bioavailability in pelleted and extruded diets with red drum Sciaenops ocellatus SO AQUACULTURE LA English DT Article; Proceedings Paper CT 16th International Symposium on Fish Nutrition and Feeding (ISFNF) CY MAY 25-30, 2014 CL Cairns, AUSTRALIA DE Red drum (Sciaenops ocellatus); Taurine; Bioavailability; Extrusion processing ID FLOUNDER PARALICHTHYS-OLIVACEUS; DISTILLERS DRIED GRAINS; TROUT ONCORHYNCHUS-MYKISS; BREAM PAGRUS-MAJOR; JAPANESE FLOUNDER; GROWTH-PERFORMANCE; RAINBOW-TROUT; SERIOLA-QUINQUERADIATA; ESSENTIAL NUTRIENT; GROWING PIGS AB Taurine has been reported to be efficacious in supporting growth of carnivorous fish species, particularly when supplemented to diets primarily containing plant feedstuffs. Although taurine may become unavailable to some extent by heat and moisture, and is susceptible to theMaillard reaction with reducing sugars, information is lacking on the stability and bioavailability of taurine subjected to extrusion processing in the manufacture of compounded fish feeds. Therefore, a feeding trial was conducted with juvenile red drum to determine the bioavailability of synthetic taurine in diets processed by cold pelleting and extrusion technology. Experimental diets were formulated without animal ingredients to contain 38% digestible protein and 20% lipid. Diets were supplemented with taurine at either 0, 0.5, 1, 2 or 4% of diet. Half of each diet was processed using conventional cooking extrusion conditions, and the other half of each diet was processed at cold temperatures, with minimal shear and pressure, resulting in 10 experimental diets. Taurine was very stable to manufacturing by cold extrusion or extrusion cooking. Post- extrusion taurine concentration was 98.9% of target values with a coefficient of variation of 2.4%. Groups of 12 juvenile red drum (averaging 4.3 g/fish) were sorted by size and stocked into each of 30, 38-L aquaria as part of an indoor brackish water (6-7 ppt) recirculating system. Each diet was fed to three replicate groups of fish twice daily at rates close to apparent satiation for 6 weeks. Fish were group weighed weekly to adjust feeding rations. At the end of the trial, three fish per aquarium were collected for determination of body condition indices and whole-body proximate composition, as well as basal taurine concentrations in plasma and muscle. Three additional fish per aquarium were bled at 5 h post-feeding for determination of post-prandial plasma taurine concentrations. Best performance of red drum in weight gain and feed efficiency was observed with the supplementation of taurine up to 1% of diet while proximate composition and condition indices were not affected by dietary taurine. Plasma and muscle taurine concentrations showed that extrusion processing significantly reduced the bioavailability of taurine. Slope ratio analysis of plasma taurine data indicated cooking extrusion reduced taurine bioavailability by 36.6%. This study, demonstrated that red drum gained more weight when fed plant-based diets supplemented with taurine regardless of diet processing technique. Statement of Relevance Tuarine supplementation to plant-based diets for carnivorous fish species is becoming more common. This study assessed the bioavailability of taurine in cold pelleted and extrusion processed diets to red drum, and found that extrusion processing reduced taurine bioavailability by approximately 37% compared to cold pelleting. (C) 2015 Elsevier B.V. All rights reserved. C1 [Velasquez, Alejandro; Pohlenz, Camilo; Gatlin, Delbert M., III] Texas A&M Univ Syst, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. [Gaylord, T. Gibson] US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Barrows, Frederic T.] ARS, USDA, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. RP Gatlin, DM (reprint author), Texas A&M Univ Syst, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. EM d-gatlin@tamu.edu FU Soy Aquaculture Alliance FX The research reported here was funded by a grant from the Soy Aquaculture Alliance. That financial support is gratefully acknowledged. NR 40 TC 1 Z9 1 U1 4 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD DEC 1 PY 2015 VL 449 BP 2 EP 7 DI 10.1016/j.aquaculture.2015.03.034 PG 6 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CU4FU UT WOS:000363483400002 ER PT J AU Stegelmeier, BL Brown, AW Welch, KD AF Stegelmeier, Bryan L. Brown, Ammon W. Welch, Kevin D. TI Safety concerns of herbal products and traditional Chinese herbal medicines: dehydropyrrolizidine alkaloids and aristolochic acid SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Review DE dehydropyrrolizidine alkaloids; pyrrolizidine alkaloids; aristolochic acid; herb; herbal products; herbal medicines; traditional Chinese medicines ID PYRROLIZIDINE ALKALOIDS; VENOOCCLUSIVE DISEASE; CARCINOGENIC ACTIVITY; DIETARY-SUPPLEMENTS; TOXICITY; PLANTS; RATS; FOOD; METABOLISM; EPIDEMIC AB In many countries, including the United States, herbal supplements, tisanes and vegetable products, including traditional Chinese medicines, are largely unregulated and their content is not registered, monitored or verified. Consequently, potent plant toxins including dehydropyrrolizidine alkaloids and other potential carcinogens can contaminate these products. As herbal and food supplement producers are left to their own means to determine the safety and purity of their products prior to marketing, disturbingly often good marketing practices currently in place are ignored and content is largely undocumented. Historical examples of poisoning and health issues relating to plant material containing dehydopyrrolizidine alkaloids and aristolochic acids were used as examples to demonstrate the risk and potential toxicity of herbal products, food supplements, or traditional medicines. More work is needed to educate consumers of the potential risk and require the industry to be more responsible to verify the content and insure the safety of their products. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. In many countries, herbal supplements, tisanes and vegetable products, including traditional Chinese medicines, are unregulated and their content is not registered, monitored or verified. Consequently, plant toxins including dehydropyrrolizidine alkaloids can contaminate these products. Dehydopyrrolizidine alkaloids and aristolochic acids are examples that demonstrate the risk and potential toxicity of contaminated, mislabeled or misused products. Work is, therefore, needed to educate consumers of the potential risk and to require the industry to be more responsible to verify content and insure product safety. C1 [Stegelmeier, Bryan L.; Brown, Ammon W.; Welch, Kevin D.] USDA ARS, Poisonous Plant Res Lab, North Logan, UT 84341 USA. RP Stegelmeier, BL (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 East 1400, North Logan, UT 84341 USA. EM Bryan.stegelmeier@ars.usda.gov NR 52 TC 1 Z9 1 U1 8 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD DEC PY 2015 VL 35 IS 12 BP 1433 EP 1437 DI 10.1002/jat.3192 PG 5 WC Toxicology SC Toxicology GA CU2HE UT WOS:000363343800002 PM 26152912 ER PT J AU Brown, AW Stegelmeier, BL Colegate, SM Panter, KE Knoppel, EL Hall, JO AF Brown, Ammon W. Stegelmeier, Bryan L. Colegate, Steven M. Panter, Kip E. Knoppel, Edward L. Hall, Jeffery O. TI Heterozygous p53 knockout mouse model for dehydropyrrolizidine alkaloid-induced carcinogenesis SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE pyrrolizidine alkaloid; p53 knockout; carcinogenesis; angiectasis; carcinogenesis; rodent model; hemangiosarcoma; riddelliine ID GROUNDSEL SENECIO-RIDDELLII; BIG BLUE RATS; PYRROLIZIDINE ALKALOIDS; SYMPHYTUM-OFFICINALE; ISLET-CELL; TUMORS; LIVER; DEHYDRORETRONECINE; TOXICITY; MICE AB Dehydropyrrolizidine alkaloids (DHPA) are a large, structurally diverse group of plant-derived protoxins that are potentially carcinogenic. With worldwide significance, these alkaloids can contaminate or be naturally present in the human food supply. To develop a small animal model that may be used to compare the carcinogenic potential of the various DHPAs, male heterozygous p53 knockout mice were administered a short-term treatment of riddelliine 5, 15 or 45 mg kg(-1) bodyweight day(-1) by oral gavage for 14 days, or dosed a long-term treatment of riddelliine 1 mg kg(-1) bodyweight day(-1) in pelleted feed for 12 months. Exposure to riddelliine increased the odds of tumor development in a dose-responsive manner (odds ratio 2.05 and Wald 95% confidence limits between 1.2 and 3.4). The most common neoplastic process was hepatic hemangiosarcoma, which is consistent with published lifetime rodent riddelliine carcinogenesis studies. Angiectasis (peliosis hepatis) and other previously unreported lesions were also identified. The results of this research demonstrate the utility of the heterozygous p53 knockout mouse model for further investigation of comparative carcinogenesis of structurally and toxicologically different DHPAs and their N-oxides. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. Dehydropyrrolizidine alkaloids (DHPAs) are a large, structurally diverse, potentially carcinogenic group of plant-derived protoxins that are common food contaminates. We utilized a heterozygous p53 knockout mouse model to compare the carcinogenic potential of various DHPAs. Exposure to riddelliine, a model DHPA, increased the odds of tumor development (odds ratio 2.05 and Wald 95% confidence limits between 1.2 and 3.4). Our research demonstrates the utility of this model for investigation of comparative carcinogenesis of different DHPAs and their N-oxides. C1 [Brown, Ammon W.; Stegelmeier, Bryan L.; Colegate, Steven M.; Panter, Kip E.; Knoppel, Edward L.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Hall, Jeffery O.] Utah State Univ, Utah Vet Diagnost Lab, Logan, UT 84322 USA. RP Brown, AW (reprint author), USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. EM brown.ammon@gmail.com NR 35 TC 3 Z9 3 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD DEC PY 2015 VL 35 IS 12 BP 1557 EP 1563 DI 10.1002/jat.3120 PG 7 WC Toxicology SC Toxicology GA CU2HE UT WOS:000363343800015 PM 25690669 ER PT J AU Moehninsi Navarre, DA Brown, CR AF Moehninsi Navarre, Duroy A. Brown, Chuck R. TI Phytonutrient content of Solanum sisymbriifolium Lam. berries SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Food analysis; Food composition; Solanum sysymbrifollium (Lam.); Litchi tomato; Potato cyst nematode; Phytonutrients; Antioxidants; Chlorogenic acid; Carotenoids ID POTATO CYST NEMATODES; CHLOROGENIC ACIDS; ANTIOXIDANT ACTIVITIES; PHYSALIS-IXOCARPA; TOTAL PHENOLICS; TRAP CROPS; FRUITS; CAROTENOIDS; TOMATOES; CULTIVARS AB Solanum sisymbriifolium Lam. (Litchi tomato) is grown ornamentally, and in Europe it is used as a trap crop for management of the potato cyst nematode (PCN). Its berries are edible, but little is known about their nutritional content. If more was known about their nutritional value this could provide incentive to grow it as a food crop. Phytonutrient content was characterized in berries from four varieties and four synthetic breeding lines developed to have reduced spininess. Litchi tomatoes contained 6.8-10.4 mg of total phenolics per g dry weight. Antioxidants measured by FRAP ranged from 148 to 242 mu mol TE/g DW. HPLC analysis showed chlorogenic acid (1856-4385 mu g/g DW) was the most abundant phenylpropanoid. Ascorbic acid ranged from 2042 to 4511 mu g/g DW. The yellow/orange flesh color was due to carotenoids, with beta-carotene the most abundant (204-633 mu g/g DW). Soluble protein in Litchi tomato ranged from 86.9 to 120.9 mg/g. Of the cultivated Litchi tomato varieties analyzed, SS91 had the highest amount of antioxidant activity, ascorbic acid, chlorogenic acid and beta-carotene. These results suggest that Litchi tomato fruits can be a good source of phytonutrients, expanding the plant's functionality beyond its use as a PCN trap crop. Published by Elsevier Inc. C1 [Moehninsi] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. [Navarre, Duroy A.; Brown, Chuck R.] USDA ARS, Prosser, WA 99350 USA. [Navarre, Duroy A.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Navarre, DA (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. EM navarrer@wsu.edu FU USDA-APHIS FX The authors thank USDA-APHIS for financial support. NR 54 TC 0 Z9 0 U1 2 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD DEC PY 2015 VL 44 BP 73 EP 79 DI 10.1016/j.jfca.2015.07.004 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CU2JA UT WOS:000363348600008 ER PT J AU Johnson, CR Thavarajah, D Thavarajah, P Fenlason, A Mcgee, R Kumar, S Combs, GF AF Johnson, Casey R. Thavarajah, Dil Thavarajah, Pushparajah Fenlason, Abby Mcgee, Rebecca Kumar, Shiv Combs, Gerald F., Jr. TI A global survey of low-molecular weight carbohydrates in lentils SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Food analysis; Food composition; Lens culinaris (lentil); Complex carbohydrates; Raffinose-family oligosaccharides; Fructooligosaccharides; Water-deficit stress; Nutrition quality ID DESICCATION TOLERANCE; GENOTYPES; ARABIDOPSIS; RESPONSES; SUGARS; SEEDS; MEDIK AB Lentils contain a range of low-molecular weight carbohydrates (LMWC); however, those have not been well characterized. The objectives of this study were to (1) determine the concentrations of LMWC in lentils grown in six locations, and (2) identify any genetic and environmental effects on those LMWC concentrations. We analyzed 335 samples from 10 locations throughout 6 countries using high-performance liquid chromatography for sugar alcohols and various mono-, di-, and oligo-saccharides, including raffinose-family oligosaccharides (RFO) and fructooligosaccharides (FOS). Mean LMWC concentrations from each country varied widely: sorbitol, 1250-1824 mg/100 g; mannitol, 57-132 mg/100 g; galactinol, 46-89 mg/100 g; sucrose, 1750-2355 mg/100 g; raffinose + stachyose, 3314-4802 mg/100 g; verbascose, 1907-2453 mg/100 g; nystose, 8-450 mg/100 g; and kestose, from not detected to 244 mg/100 g. The concentrations of many of these LMWC varied with average temperature and precipitation of the region/country of origin. Significant genotype and genotype x location effects contributed to the variability in the concentrations of several LMWC, a feature that could prove useful in breeding lentils for novel agro-ecological environments and for consumer preference. The range of LMWC in lentil may contribute to its survival as a crop and its organoleptic and nutritional properties as a whole food. (C) 2015 Elsevier Inc. All rights reserved. C1 [Johnson, Casey R.] Mayo Clin & Mayo Grad Sch Med, Rochester, MN 55905 USA. [Thavarajah, Dil; Thavarajah, Pushparajah] Clemson Univ, Poole Agr Ctr 270, Dept Agr & Environm Sci, Clemson, SC 29634 USA. [Fenlason, Abby] N Dakota State Univ, Sch Food Syst, Fargo, ND 58108 USA. [Mcgee, Rebecca] Washington State Univ, USDA ARS, Grain Legume Genet & Physiol Unit, Pullman, WA 99164 USA. [Kumar, Shiv] ICARDA, Biodivers & Integrated Gene Management Program, Rabat, Morocco. [Combs, Gerald F., Jr.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Thavarajah, D (reprint author), Clemson Univ, Poole Agr Ctr 270, Dept Agr & Environm Sci, Clemson, SC 29634 USA. EM dthavar@clemson.edu FU Northern Pulse Growers Association, ND, USA; USA Dry Pea Lentil Council, USA FX Support for this research was provided by the Northern Pulse Growers Association, ND, USA and USA Dry Pea Lentil Council, USA. NR 27 TC 2 Z9 2 U1 3 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD DEC PY 2015 VL 44 BP 178 EP 185 DI 10.1016/j.jfca.2015.08.005 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CU2JA UT WOS:000363348600021 ER PT J AU Li, GY Hse, CY Qin, TF AF Li, Gaiyun Hse, Chungyun Qin, Tefu TI Wood liquefaction with phenol by microwave heating and FTIR evaluation SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Wood liquefaction; Phenol; Microwave heating; Oil bath heating; Fourier transform infrared spectroscopy (FTIR) ID LIQUEFIED WOOD; ACID; CELLULOSE; SPECTROSCOPY; PYROLYSIS; RESIN; TIME AB We examined wood liquefaction using phenol and mixed acid catalysts with microwave heating, and compared that with similar processes that use oil bath heating. The reaction time for microwave heating to achieve a residue content was one sixth, one eighteenth, and one twenty-fourth of that from oil bath heating, respectively, for phenol to wood (P/W) ratios of 2.5/1, 2/1 and 1.5/1. A low P/W ratio tended to result in carbonization of liquefied wood due to an insufficient amount of phenol and localized microwave superheating. Fourier transform infrared spectroscopic (FTIR) evaluation of the liquefied residue, showed that the liquefaction rates of wood components differed. Hemicellulose was most susceptible to liquefaction, crystalline cellulose was most recalcitrant, and guaiacyl units the most prone to re-condensation. From FTIR, the chemical components and substitution patterns of bonded phenol were similar for both methods. C1 [Li, Gaiyun; Qin, Tefu] Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China. [Hse, Chungyun] USDA, Forest Serv, Southern Res Stn, Pineville, LA 71360 USA. RP Li, GY (reprint author), Chinese Acad Forestry, Res Inst Wood Ind, Xiangshan Rd, Beijing 100091, Peoples R China. EM ligy@caf.ac.cn FU "948 Project" of State Forestry Administration [2012-4-28] FX The authors gratefully acknowledge the Southern Research Station, USDA Forest Service, USA for kindly providing the experimental facilities for this study. The financial support of "948 Project" of State Forestry Administration (2012-4-28) is gratefully acknowledged. NR 23 TC 2 Z9 2 U1 1 U2 8 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD DEC PY 2015 VL 26 IS 4 BP 1043 EP 1048 DI 10.1007/s11676-015-0114-0 PG 6 WC Forestry SC Forestry GA CU1BW UT WOS:000363255100028 ER PT J AU Hessburg, PF Churchill, DJ Larson, AJ Haugo, RD Miller, C Spies, TA North, MP Povak, NA Belote, RT Singleton, PH Gaines, WL Keane, RE Aplet, GH Stephens, SL Morgan, P Bisson, PA Rieman, BE Salter, RB Reeves, GH AF Hessburg, Paul F. Churchill, Derek J. Larson, Andrew J. Haugo, Ryan D. Miller, Carol Spies, Thomas A. North, Malcolm P. Povak, Nicholas A. Belote, R. Travis Singleton, Peter H. Gaines, William L. Keane, Robert E. Aplet, Gregory H. Stephens, Scott L. Morgan, Penelope Bisson, Peter A. Rieman, Bruce E. Salter, R. Brion Reeves, Gordon H. TI Restoring fire-prone Inland Pacific landscapes: seven core principles SO LANDSCAPE ECOLOGY LA English DT Review DE Forest and rangeland restoration; Hierarchical organization; Large fires; Patch size distributions; Successional patches; Topographic controls ID MIXED-CONIFER FORESTS; WESTERN UNITED-STATES; PONDEROSA PINE FOREST; NORTHERN ROCKY-MOUNTAINS; CLIMATE-CHANGE; SPATIAL-PATTERNS; SIERRA-NEVADA; ECOLOGICAL-SYSTEMS; SILVICULTURAL PRESCRIPTIONS; VEGETATION DISTRIBUTION AB Context More than a century of forest and fire management of Inland Pacific landscapes has transformed their successional and disturbance dynamics. Regional connectivity of many terrestrial and aquatic habitats is fragmented, flows of some ecological and physical processes have been altered in space and time, and the frequency, size and intensity of many disturbances that configure these habitats have been altered. Current efforts to address these impacts yield a small footprint in comparison to wildfires and insect outbreaks. Moreover, many current projects emphasize thinning and fuels reduction within individual forest stands, while overlooking large-scale habitat connectivity and disturbance flow issues. Methods We provide a framework for landscape restoration, offering seven principles. We discuss their implication for management, and illustrate their application with examples. Results Historical forests were spatially heterogeneous at multiple scales. Heterogeneity was the result of variability and interactions among native ecological patterns and processes, including successional and disturbance processes regulated by climatic and topographic drivers. Native flora and fauna were adapted to these conditions, which conferred a measure of resilience to variability in climate and recurrent contagious disturbances. Conclusions To restore key characteristics of this resilience to current landscapes, planning and management are needed at ecoregion, local landscape, successional patch, and tree neighborhood scales. Restoration that works effectively across ownerships and allocations will require active thinking about landscapes as socio-ecological systems that provide services to people within the finite capacities of ecosystems. We focus attention on landscape-level prescriptions as foundational to restoration planning and execution. C1 [Hessburg, Paul F.; Povak, Nicholas A.; Singleton, Peter H.; Salter, R. Brion] USDA Forest Serv, Pacific NW Res Stn, Wenatchee, WA 98801 USA. [Churchill, Derek J.] Univ Washington, Coll Environm, Seattle, WA 98195 USA. [Larson, Andrew J.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Haugo, Ryan D.] Nature Conservancy, Yakima, WA 98901 USA. [Miller, Carol] USDA Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Spies, Thomas A.; Reeves, Gordon H.] USDA Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [North, Malcolm P.] USDA Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Belote, R. Travis] Wilderness Soc, Bozeman, MT 59715 USA. [Gaines, William L.] Washington Conservat Sci Inst, Leavenworth, WA 98826 USA. [Keane, Robert E.] USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59808 USA. [Aplet, Gregory H.] Wilderness Soc, Denver, CO 80202 USA. [Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Morgan, Penelope] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Bisson, Peter A.] USDA Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Hessburg, PF (reprint author), USDA Forest Serv, Pacific NW Res Stn, 1133 N Western Ave, Wenatchee, WA 98801 USA. EM phessburg@fs.fed.us FU PSW Research Stations; Joint Fire Sciences Program; National Science Foundation [1256819]; National Fire Plan FX The authors thank Keith Reynolds, Cameron Thomas, Richy Harrod, Rachel White, and the anonymous reviewers for helpful reviews. We thank James Pass and the staff from the Three Rivers Ranger District on the Colville NF for working with us on the landscape evaluation and prescription for the Orient Watershed. We also thank the PNW, Rocky Mountain, and PSW Research Stations, the Joint Fire Sciences Program, National Science Foundation (Award #1256819), and the National Fire Plan for generous support of the many research studies synthesized in this review. NR 196 TC 24 Z9 23 U1 15 U2 74 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 1805 EP 1835 DI 10.1007/s10980-015-0218-0 PG 31 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900001 ER PT J AU Shirk, AJ Schroeder, MA Robb, LA Cushman, SA AF Shirk, Andrew J. Schroeder, Michael A. Robb, Leslie A. Cushman, Samuel A. TI Empirical validation of landscape resistance models: insights from the Greater Sage-Grouse (Centrocercus urophasianus) SO LANDSCAPE ECOLOGY LA English DT Article DE Centrocercus urophasianus; Greater Sage-Grouse; Landscape genetics; Lek; Resistance; Validation ID MANTEL TEST; GENE FLOW; REGRESSION; DISPERSAL; INFERENCE; PATTERNS; SCALE; TESTS AB Context The ability of landscapes to impede species' movement or gene flow may be quantified by resistance models. Few studies have assessed the performance of resistance models parameterized by expert opinion. In addition, resistance models differ in terms of spatial and thematic resolution as well as their focus on the ecology of a particular species or more generally on the degree of human modification of the landscape (i.e. landscape integrity). The effect of these design decisions on model accuracy is poorly understood. Objectives We sought to understand the influence of expert parameterization, resolution, and specificity (i.e. species-specific or landscape integrity) on the fit of resistance model predictions to empirical landscape patterns. Methods With genetic and observational data collected from Greater Sage-Grouse (Centrocercus urophasianus) in Washington State, USA, we used landscape genetic analysis and logistic regression to evaluate a range of resistance models in terms of their ability to predict empirical patterns of genetic differentiation and lek occupancy. Results We found that species-specific, fine resolution resistance models generally had stronger relationships to empirical patterns than coarse resolution or landscape integrity models, and that the expert models were less predictive than alternative parameterizations. Conclusions Our study offers an empirical framework to validate expert resistance models, suggests the need to match the grain of the data to the scale at which the species responds to landscape heterogeneity, and underscores the limitations of landscape integrity models when the species under study does not meet their assumptions. C1 [Shirk, Andrew J.] Univ Washington, Climate Impacts Grp, Seattle, WA 98195 USA. [Schroeder, Michael A.] Washington Dept Fish & Wildlife, Bridgeport, WA 98813 USA. [Cushman, Samuel A.] USDA Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Shirk, AJ (reprint author), Univ Washington, Climate Impacts Grp, POB 355672, Seattle, WA 98195 USA. EM ashirk@uw.edu; michael.schroeder@dfw.wa.gov; robblar@homenetnw.net; scushman@fs.fed.us NR 36 TC 3 Z9 3 U1 0 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 1837 EP 1850 DI 10.1007/s10980-015-0214-4 PG 14 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900002 ER PT J AU Moriarty, KM Epps, CW Betts, MG Hance, DJ Bailey, JD Zielinski, WJ AF Moriarty, Katie M. Epps, Clinton W. Betts, Matthew G. Hance, Dalton J. Bailey, J. D. Zielinski, William J. TI Experimental evidence that simplified forest structure interacts with snow cover to influence functional connectivity for Pacific martens SO LANDSCAPE ECOLOGY LA English DT Article DE Connectivity; Forest management; Fuel reduction; Functional connectivity; Landscape fragmentation; Martes americana; Martes caurina; Marten; Movement; Titration experiment ID AMERICAN MARTEN; HABITAT FRAGMENTATION; PREDATION RISK; LANDSCAPE; CALIFORNIA; WISCONSIN; SELECTION; ECOLOGY; BIRDS; FIRE AB Context Functional connectivity-the facilitation of individual movements among habitat patches-is essential for species' persistence in fragmented landscapes. Evaluating functional connectivity is critical for predicting range shifts, developing conservation plans, and anticipating effects of disturbance, especially for species affected by climate change. Objectives We examined whether simplifying forest structure influenced animal movements and whether an experimental approach to quantifying functional connectivity reflects normal behavior, which is often assumed but remains untested. Methods We evaluated functional connectivity for Pacific marten (Martes caurina) across a gradient in forest structural complexity using two novel methods for this species: incentivized food-titration experiments and non-incentivized locations collected via GPS telemetry (24 individuals). Results Food titration experiments revealed martens selected complex stands, and martens entered and crossed areas with reduced forest cover when motivated by bait, particularly in the winter. However, our telemetry data showed that without such incentive, martens avoided openings and simple stands and selected complex forest stands equally during summer and winter. Conclusions Detections at baited stations may not represent typical habitat preferences during winter, and incentivized experiments reflect the capacity of martens to enter non-preferred stand types under high motivation (e.g., hunger, curiosity, dispersal). We hypothesize snow cover facilitates connectivity across openings when such motivation is present; thus, snow cover may benefit dispersing animals and increase population connectivity. Landscapes with joined networks of complex stands are crucial for maintaining functional connectivity for marten, particularly during summer. C1 [Moriarty, Katie M.; Epps, Clinton W.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Betts, Matthew G.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Hance, Dalton J.] Anchor QEA LLC, Nat Resources, Wenatchee, WA 98801 USA. [Bailey, J. D.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Zielinski, William J.] US Forest Serv, Pacific Southwest Res Stn, Arcata, CA 95521 USA. RP Moriarty, KM (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. EM ktmoriarty22@gmail.com FU Lassen National Forest and Almanor Ranger District, California; Oregon State University (OSU) Laurels Block Grant; Western Section of the Wildlife Society (TWS); OSU Department of Fisheries and Wildlife; Oregon Chapter-TWS; Mountain Hardwear; Madshus Skis; OSU Fisheries and Wildlife; Thomas G. Scott Fund FX Funding and field support were provided by the Lassen National Forest and Almanor Ranger District, California, as well as Oregon State University (OSU) Laurels Block Grant, the Western Section of the Wildlife Society (TWS), the OSU Department of Fisheries and Wildlife, and the Oregon Chapter-TWS, Mountain Hardwear, and Madshus Skis. Publication fees were supported by the OSU Fisheries and Wildlife Thomas G. Scott Fund. We thank M. Williams and T. Frolli and the staff at Almanor Ranger District, and C. Dillingham, R. Martinez, and S. VanPetten for assistance with mapping management activities. We thank A. Moffett, the Epps and Betts labs, the staff at OSU for logistics support. We appreciated assistance improving this manuscript from anonymous reviewers. Lastly, we thank our field technicians (M. Linnell, M. Delheimer, L. Kreiensieck, P. Tweedy, R. Adamczyk, K. Mansfield, B. Barry, B. Peterson, D. Hamilton, C. Wood, M. Dao, M. Cokeley), and the many volunteers on this project. NR 69 TC 2 Z9 2 U1 5 U2 53 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 1865 EP 1877 DI 10.1007/s10980-015-0216-2 PG 13 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900004 ER PT J AU Schneiderman, JE He, HS Thompson, FR Dijak, WD Fraser, JS AF Schneiderman, Jeffrey E. He, Hong S. Thompson, Frank R., III Dijak, William D. Fraser, Jacob S. TI Comparison of a species distribution model and a process model from a hierarchical perspective to quantify effects of projected climate change on tree species SO LANDSCAPE ECOLOGY LA English DT Article DE Climate change; LINKAGES 2.2; Climate Change Tree Atlas; Hierarchical; Process model; Species distribution model ID EASTERN UNITED-STATES; CHANGE IMPACTS; LANDSCAPE ECOLOGY; RANGE SHIFTS; FORESTS; UNCERTAINTY; ABUNDANCE; PREDICTIONS; SUCCESSION; PATTERNS AB Context Tree species distribution and abundance are affected by forces operating across a hierarchy of ecological scales. Process and species distribution models have been developed emphasizing forces at different scales. Understanding model agreement across hierarchical scales provides perspective on prediction uncertainty and ultimately enables policy makers and managers to make better decisions. Objective Our objective was to test the hypothesis that agreement between process and species distribution models varies by hierarchical level. Due to the top-down approach of species distribution models and the bottom-up approach of process models, the most agreement will occur at the mid-level of the hierarchical analysis, the ecological subsection level, capturing the effects of soil variables. Methods We compared projections of a species distribution model, Climate Change Tree Atlas, and a process model, LINKAGES 2.2. We conducted a correlation analysis between the models at regional, ecological subsection, and species level hierarchical scales. Results Both models had significant positive correlation (rho = 0.53, P < 0.001) on the regional scale. The majority of the ecological subsections had greater model correlation than on the regional level when all climate scenarios were pooled. Correlation was poorest for the analysis of individual species. Models had the greatest correlation at the regional scale for the GFDL-A1fi scenario (the scenario with the most climate change). Species near their range edge generally had stronger correlation (loblolly pine, northern red oak, black oak). Conclusion Our general hypothesis was partly accepted. This suggests that uncertainties are relatively low when interpreting model results at subsection level. C1 [Schneiderman, Jeffrey E.; He, Hong S.; Fraser, Jacob S.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Thompson, Frank R., III; Dijak, William D.] Univ Missouri, USDA Forest Serv, No Res Stn, Columbia, MO 65211 USA. RP Schneiderman, JE (reprint author), Univ Missouri, Dept Forestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM jesg37@mail.missouri.edu FU U.S.D.A. Forest Service Northern Research Station; University of Missouri GIS Mission Enhancement Program; Department of Interior U.S.G.S. Northeast Climate Science Center FX We would like to thank Louis R. Iverson and Matthew P. Peters for providing us with Climate Change Tree Atlas raw data for our analysis. We would like to thank Stan D. Wullschleger for input and clarification regarding LINKAGES 2.2. We would like to thank Wilfred M. Post for input regarding the LINKAGES model. We would also like to thank two anonymous reviewers. Funding for this project was provided by the U.S.D.A. Forest Service Northern Research Station, the University of Missouri GIS Mission Enhancement Program, and the Department of Interior U.S.G.S. Northeast Climate Science Center. The contents of this paper are solely the responsibility of the authors and do not necessarily represent the views of the United States Government. NR 53 TC 0 Z9 0 U1 4 U2 42 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 1879 EP 1892 DI 10.1007/s10980-015-0217-1 PG 14 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900005 ER PT J AU Behrman, KD Juenger, TE Kiniry, JR Keitt, TH AF Behrman, Kathrine D. Juenger, Thomas E. Kiniry, James R. Keitt, Timothy H. TI Spatial land use trade-offs for maintenance of biodiversity, biofuel, and agriculture SO LANDSCAPE ECOLOGY LA English DT Article DE Species richness; Ecosystem services; Switchgrass; Spatial optimization; ALMANAC model; Panicum virgatum L. ID CLIMATE-CHANGE; UNITED-STATES; SWITCHGRASS; BIOENERGY; ENERGY; CONSEQUENCES; DIVERSITY; SCENARIOS; RESPONSES; BENEFITS AB Expansion of bioenergy production is part of a global effort to reduce greenhouse gas emissions and mitigate climate change. Dedicated biomass crops will compete with other land uses as most high quality arable land is already used for agriculture, urban development, and biodiversity conservation. First, we explore the trade-offs between converting land enrolled in the U.S. Conservation Reserve Program (CRP) to switchgrass for biofuel production or preserving it for biodiversity. Next, we examine the trade-offs between agriculture, biodiversity, and biofuel across the central and eastern U.S. We compiled measures of biodiversity, agriculture, and biofuel from land cover classifications, species range maps, and mechanistic model output of switchgrass yield. We used a spatially-explicit optimization algorithm to analyze the impacts of small-to-large scale biomass production by identifying locations that maximize biofuel produced from switchgrass and minimize negative impacts on biodiversity and agriculture. Using CRP land for switchgrass production increases the land area required to meet biomass goals and the species range area altered for birds, amphibians, mammals, and reptiles. When conversion is not limited to CRP, conversion scenarios including biodiversity and agriculture trade-offs require greater than 100 % more area for switchgrass to reach the same production goals. When land conversion scenarios do not include biodiversity, twice the range area for reptiles and amphibians could be altered. Land-use trade-offs between biofuel production, agriculture, and biodiversity exist and alter optimum location of land conversion for low-to-high biofuel levels. This highlights the need for systematic land-use planning for the future. C1 [Behrman, Kathrine D.; Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Behrman, Kathrine D.; Juenger, Thomas E.; Keitt, Timothy H.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. RP Behrman, KD (reprint author), USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. EM kate.behrman@ars.usda.gov FU National Science Foundation Plant Genome Research Program [NSF IOS-0922457] FX We thank Daren Harmel, Mari-Vaughn Johnson, Norman Meki, and Wayne Polley for helpful comments. Funding for this works was provided by The National Science Foundation Plant Genome Research Program award number NSF IOS-0922457. USDA is an equal opportunity provider and employer. NR 49 TC 0 Z9 0 U1 8 U2 81 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 1987 EP 1999 DI 10.1007/s10980-015-0225-1 PG 13 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900012 ER PT J AU Gutzwiller, KJ Riffell, SK Flather, CH AF Gutzwiller, Kevin J. Riffell, Samuel K. Flather, Curtis H. TI Avian abundance thresholds, human-altered landscapes, and the challenge of assemblage-level conservation SO LANDSCAPE ECOLOGY LA English DT Article DE Abrupt spatial changes; Bird-landscape thresholds; Geographic ranges; Internal structure; Landscape planning and management; Threshold pervasiveness and diversity ID ECOLOGICAL THRESHOLDS; HABITAT LOSS; RELATIVE INFLUENCE; TERRESTRIAL BIRDS; UNITED-STATES; FRAGMENTATION; FOREST; PATTERNS; OCCUPANCY; DIVERSITY AB Land-use change is a global phenomenon with potential to generate abrupt spatial changes in species' distributions. We assessed whether theory about the internal structure of bird species' geographic ranges can be refined to reflect abrupt changes in distribution and abundance associated with human influences on landscapes, and whether the prevalence and diversity of bird-landscape threshold relationships may significantly complicate assemblage-level avian conservation. For three large regions in the United States, we used the North American Breeding Bird Survey, U.S. National Land Cover Data, and multivariate adaptive regression splines to assess whether land bird species' abundances were associated with landscape composition and configuration in a threshold fashion. Threshold relationships between abundance and landscape characteristics were exhibited by 42-60 % of the species studied. The relationships were evident for five land types and five habitat guilds. We observed threshold relationships for more taxonomically diverse groups of bird species, a broader set of land types, and larger geographic extents than have been considered to date. Avian distribution and abundance theory can be refined by articulating that characteristics of human-altered landscapes have the potential to be widespread and biologically important contributors to abrupt spatial change in species' abundances. Our findings also expose bird-landscape threshold relationships as pervasive and diverse patterns that impose a much more complicated set of circumstances for assemblage-level conservation of birds than has been widely recognized. To cope with these complications, landscape planners and managers can use optimization analyses, multispecies frameworks, regulatory limits, and multivariate change-point analyses. C1 [Gutzwiller, Kevin J.] Baylor Univ, Dept Biol, Waco, TX 76798 USA. [Riffell, Samuel K.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Flather, Curtis H.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. RP Gutzwiller, KJ (reprint author), Baylor Univ, Dept Biol, Waco, TX 76798 USA. EM kevin_gutzwiller@baylor.edu FU Rocky Mountain Research Station, Forest Service, U.S. Department of Agriculture [09-JV-11221636-208]; Baylor University FX This research was supported in part by funds provided by the Rocky Mountain Research Station, Forest Service, U.S. Department of Agriculture (Agreement 09-JV-11221636-208), and by funds provided by Baylor University. Kevin J. Gutzwiller's work on this project was supported by these funds. NR 69 TC 2 Z9 2 U1 8 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 2095 EP 2110 DI 10.1007/s10980-015-0233-1 PG 16 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900019 ER PT J AU Krosby, M Breckheimer, I Pierce, DJ Singleton, PH Hall, SA Halupka, KC Gaines, WL Long, RA Mcrae, BH Cosentino, BL Schuett-Hames, JP AF Krosby, Meade Breckheimer, Ian Pierce, D. John Singleton, Peter H. Hall, Sonia A. Halupka, Karl C. Gaines, William L. Long, Robert A. McRae, Brad H. Cosentino, Brian L. Schuett-Hames, Joanne P. TI Focal species and landscape "naturalness" corridor models offer complementary approaches for connectivity conservation planning SO LANDSCAPE ECOLOGY LA English DT Article DE Coarse-filter; Connectivity; Corridors; Fine-filter; Focal-species; Landscape integrity ID TIGER SALAMANDERS; HUMAN FOOTPRINT; CLIMATE-CHANGE; SAGE GROUSE; HABITAT USE; HOME-RANGE; DISPERSAL; PRIORITIZATION; POPULATION; MANAGEMENT AB The dual threats of habitat fragmentation and climate change have led to a proliferation of approaches for connectivity conservation planning. Corridor analyses have traditionally taken a focal species approach, but the landscape "naturalness" approach of modeling connectivity among areas of low human modification has gained popularity as a less analytically intensive alternative. We compared focal species and naturalness-based corridor networks to ask whether they identify similar areas, whether a naturalness-based approach is in fact more analytically efficient, and whether agreement between the two approaches varies with focal species vagility. We compared focal-species and naturalness-based connectivity models at two nested spatial extents: greater Washington State, USA, and, within it, the Columbia Plateau ecoregion. We assessed complementarity between the two approaches by examining the spatial overlap of predicted corridors, and regressing organism traits against the amount of modeled corridor overlap. A single naturalness-based corridor network represented connectivity for a large (> 10) number of focal species as effectively as a group of between 3 and 4 randomly selected focal species. The naturalness-based approach showed only moderate spatial agreement with composite corridor networks for large numbers of focal species, and better agreed with corridor networks of large-bodied, far-dispersing species in the larger scale analysis. Naturalness-based corridor models may offer an efficient proxy for focal species models, but a multi-focal species approach may better represent the movement needs of diverse taxa. Consideration of trade-offs between the two approaches may enhance the effectiveness of their application to connectivity conservation planning. C1 [Krosby, Meade] Univ Washington, Coll Environm, Climate Impacts Grp, Seattle, WA 98195 USA. [Breckheimer, Ian] Univ Washington, Dept Biol, Seattle, WA 98195 USA. [Pierce, D. John; Cosentino, Brian L.; Schuett-Hames, Joanne P.] Washington Dept Fish & Wildlife, Olympia, WA 98516 USA. [Singleton, Peter H.] US Forest Serv, USDA, Pacific NW Res Stn, Wenatchee, WA 98801 USA. [Hall, Sonia A.] SAH Ecol LLC, Wenatchee, WA 98801 USA. [Halupka, Karl C.] US Fish & Wildlife Serv, Cent Washington Field Off, Wenatchee, WA 98801 USA. [Gaines, William L.] Washington Conservat Sci Inst, Leavenworth, WA 98826 USA. [Long, Robert A.] Woodland Pk Zoo, Seattle, WA 98103 USA. [McRae, Brad H.] Nature Conservancy, Ft Collins, CO 80524 USA. RP Krosby, M (reprint author), Univ Washington, Coll Environm, Climate Impacts Grp, Box 355674, Seattle, WA 98195 USA. EM mkrosby@uw.edu FU Wilburforce Foundation FX We would like to acknowledge the full membership of the Washington Wildlife Habitat Connectivity Working Group, especially the modelers and species leads who completed the connectivity models used in our analysis. MK received support for this analysis from the Wilburforce Foundation. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service. NR 56 TC 6 Z9 6 U1 11 U2 59 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD DEC PY 2015 VL 30 IS 10 BP 2121 EP 2132 DI 10.1007/s10980-015-0235-z PG 12 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA CU1IR UT WOS:000363274900021 ER PT J AU Cheung, WHK Pasamontes, A Peirano, DJ Zhao, WX Grafton-Cardwell, EE Kapaun, T Yokomi, RK Simmons, J Doll, M Fiehn, O Dandekar, AM Davis, CE AF Cheung, William H. K. Pasamontes, Alberto Peirano, Daniel J. Zhao, Weixiang Grafton-Cardwell, Elizabeth E. Kapaun, Therese Yokomi, Raymond. K. Simmons, Jason Doll, Mimi Fiehn, Oliver Dandekar, Abhaya M. Davis, Cristina E. TI Volatile organic compound (VOC) profiling of citrus tristeza virus infection in sweet orange citrus varietals using thermal desorption gas chromatography time of flight mass spectrometry (TD-GC/TOF-MS) SO METABOLOMICS LA English DT Article DE Volatile organic compounds (VOCs); Citrus tristeza virus (CTV); Mass spectrometry; Gas chromatography; Biomarker discovery ID BAR SORPTIVE EXTRACTION; TERPENE DOWN-REGULATION; CAPILLARY GC-MS; PLANT VOLATILES; RT-PCR; PATHOGENS; CALIFORNIA; DIVERSITY; REVEALS; DISEASE AB Citrus tristeza virus (CTV) (genus Closterovirus) is a plant pathogen which infects economically important citrus crops such as sweet oranges, mandarins, limes and grapefruit varietals. Within the last 70 years, an estimated 100 million citrus trees have been destroyed due to CTV infection worldwide. Present measures to contain CTV infection include scouts for visual assessment, and molecular analysis methods such as enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction. Volatile organic compound (VOC) profiling may offer an alternative method of disease detection. In this study, we used a "Twister (TM) aEuroe sorbent system for in-field VOC sampling. Chemical analysis was performed with thermal desorption gas chromatography time-of-flight mass spectrometry, and data were subjected to unsupervised and supervised analysis. Samples were collected from healthy trees, those with asymptomatic CTV, and those with CTV that were coinfected with a secondary unrelated bacterial infection of Spiroplasma citri, the causal agent of citrus stubborn disease (Stubborn). A total of 383 VOCs were detected across three classes: healthy control trees, CTV infected, and CTV coinfected with Stubborn. Mathematical models of this data were built to successfully differentiate: (a) healthy trees from CTV infected trees; (b) healthy trees from both CTV and CTV coinfected with Stubborn; and (c) to effectively differentiate between healthy trees and CTV infected trees, without consideration of Stubborn coinfection (the model would work on both singly or coinfected trees). The putative CTV biomarkers observed were terpenoid based (myrcene, carene, ocimene, bulnesene), two alcohols (n-undecanol, surfynol) and two acetones (geranyl acetone and neryl acetate). C1 [Cheung, William H. K.; Pasamontes, Alberto; Peirano, Daniel J.; Zhao, Weixiang; Simmons, Jason; Davis, Cristina E.] Univ Calif Davis, Dept Mech & Aerosp Engn, Davis, CA 95616 USA. [Grafton-Cardwell, Elizabeth E.; Kapaun, Therese] Univ Calif Exeter, Lindcove Res & Extens Ctr, Exeter, CA 93221 USA. [Yokomi, Raymond. K.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Doll, Mimi; Fiehn, Oliver] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Dandekar, Abhaya M.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Davis, CE (reprint author), Univ Calif Davis, Dept Mech & Aerosp Engn, One Shields Ave, Davis, CA 95616 USA. EM cedavis@ucdavis.edu RI Pasamontes Funez, Alberto/M-8515-2016; OI Pasamontes Funez, Alberto/0000-0002-4142-6337; Dandekar, Abhaya/0000-0001-7925-4086 FU California Citrus Research Board; Industry-University Cooperative Research Program; Florida Citrus Production Advisory Council; National Science Foundation [MCB 1139644]; National Institute of Food and Agriculture; US Department of Veterans Affairs [Post-9/11 G.I. Bill]; UC ANR at Lindcove Research and Extension Center (Exeter, CA) FX This manuscript is based upon work supported by the California Citrus Research Board (CED, OF, AMD), the Industry-University Cooperative Research Program (CED, OF, AMD), the Florida Citrus Production Advisory Council (CED), the National Science Foundation MCB 1139644 (OF) and the National Institute of Food and Agriculture (RKY). Student support was partially provided by the US Department of Veterans Affairs, Post-9/11 G.I. Bill (DJP). The research was supported by UC ANR at Lindcove Research and Extension Center (Exeter, CA). Opinions expressed in this publication are those of the authors and do not necessarily reflect the view of the funding agencies. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 51 TC 4 Z9 4 U1 8 U2 56 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD DEC PY 2015 VL 11 IS 6 BP 1514 EP 1525 DI 10.1007/s11306-015-0807-6 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CT8BS UT WOS:000363040600004 ER PT J AU Lokvam, J Metz, MR Takeoka, GR Nguyen, L Fine, PVA AF Lokvam, John Metz, Margaret R. Takeoka, Gary R. Lien Nguyen Fine, Paul V. A. TI Habitat-specific divergence of procyanidins in Protium subserratum (Burseraceae) SO CHEMOECOLOGY LA English DT Article DE Protium subserratum; Procyanidin; Defense chemistry; Herbivory; Habitat specialization; Speciation ID CONDENSED TANNINS; HERBIVORY; DEFENSE; LEAVES; FORESTS; PLANTS; PRECIPITATION; FRACTIONATION; RESISTANCE; PROTEINS AB In Amazonian Peru, the neotropical tree Protium subserratum Engl. (Burseraceae) occurs as distinct ecotypes on low nutrient white-sand (WS), intermediate fertility brown-sand (BS), and nutrient-rich clay (CS) soils. Genetic analysis indicates that these ecotypes are undergoing incipient speciation. Possible drivers of this divergence are habitat-specific herbivore faunas and differing resource availabilities. Protium subserratum, therefore, provides an ideal opportunity to investigate how defense chemistry evolves during lineage divergence. WS and BS races of P. subserratum are host to largely non-overlapping herbivore communities and they differ in chlorogenic acid, flavonoid, and oxidized terpene chemistry. Here, we investigate how another important class of anti-herbivore chemicals, procyanidins (PCs), varies among the ecotypes. We isolated the PCs from leaves of juvenile and adult trees from each ecotype and used spectroscopic and chemical techniques to characterize the chemical structures of their component monomers. We found that WS, BS, and CS ecotypes accumulate ca. 17 % of leaf dry weight as PCs. Within ecotypes, we found very little difference in PC type, neither by site nor by life stage. Among ecotypes, however, we observed a marked divergence in PC composition that arose at least in part from differences in their terminal and extension subunits. In addition, the average polymer length of BS and CS PCs was significantly greater than in WS ecotypes. We conclude that phenotypic differences in PCs in the WS versus BS and CS ecotypes of P. subserratum are consistent with selection by herbivores in different soil types that differ strongly in nutrient availability and may contribute to the evolution of habitat specialization. C1 [Lokvam, John; Lien Nguyen; Fine, Paul V. A.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Metz, Margaret R.] Lewis & Clark Coll, Dept Biol, Portland, OR 97219 USA. [Takeoka, Gary R.] ARS, USDA, Albany, CA 94710 USA. RP Lokvam, J (reprint author), Univ Calif Berkeley, Dept Integrat Biol, 1005 Valley Life Sci Bldg 3140, Berkeley, CA 94720 USA. EM lokvam@berkeley.edu OI Fine, Paul/0000-0002-0550-5628 FU NSF DEB [1254214] FX We thank the Ministerio del Ambiente of Peru for providing research and export permits. We thank Carlos Rivera of SERNANP-Allpahuayo-Mishana and the Instituto de Investigaciones de la Amazonia Peruana (IIAP) for institutional and logistical support. We would like to thank Italo Mesones, Magno Vasquez Pilco, J. Milagros Ayarza Zuniga, Julio Sanchez for field assistance, and Leslie Harden for high-resolution mass spectrometry. We also thank Diego Salazar Amoretti for helpful suggestions to the manuscript. Funding was provided by NSF DEB 1254214 to PVAF. NR 35 TC 2 Z9 2 U1 1 U2 15 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0937-7409 EI 1423-0445 J9 CHEMOECOLOGY JI Chemoecology PD DEC PY 2015 VL 25 IS 6 BP 293 EP 302 DI 10.1007/s00049-015-0198-1 PG 10 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CT5WT UT WOS:000362882500002 ER PT J AU Oster, M Beck, JJ Furrow, RE Yeung, K Field, CB AF Oster, Marina Beck, John J. Furrow, Robert E. Yeung, Kara Field, Christopher B. TI In-field yellow starthistle (Centaurea solstitialis) volatile composition under elevated temperature and CO2 and implications for future control SO CHEMOECOLOGY LA English DT Article DE Centaurea solstitialis (yellow starthistle); Abiotic stressors; Invasive species; Biogenic volatile organic compound (BVOC); Elevated CO2; Elevated temperature ID GLOBAL ENVIRONMENTAL-CHANGES; ISOPRENE EMISSION; BIOLOGICAL-CONTROL; NATURAL ENEMIES; CLIMATE-CHANGE; SESQUITERPENE SYNTHASES; MEDITERRANEAN SHRUBLAND; TERRESTRIAL ECOSYSTEMS; ORIENTATION BEHAVIOR; MONOTERPENE EMISSION AB Abiotic stressors may affect biogenic volatile organic compounds (BVOCs) involved in plant communication. We examined how certain environmental conditions affect plant signaling via BVOC emission. Specifically, we investigated the effects of elevated CO2 and temperature in situ on BVOCs produced by mechanically damaged leaves of Centaurea solstitialis L. (yellow starthistle), a major invasive weed in western North America, grown in grassland plots in the foothills of northern California. The headspace BVOCs of C. solstitialis were collected in situ by a customized Teflon(A (R)) bag and solid-phase microextraction and analyzed by gas chromatography-mass spectroscopy. Damaging leaves led to the release of 14 volatile compounds, predominantly sesquiterpenes. The co-occurrence of five compound pairs was highly significant across all treatments, which may be explained through synthesis by the same enzyme. We found no significant effect of treatment on the levels of individual or total volatiles. The stability of volatile profile for this invasive under future conditions should therefore (1) not alter indirect defense signaling and (2) support the selection of biological controls on the basis of their specificity to the identified in-field host plant BVOCs. C1 [Oster, Marina; Furrow, Robert E.; Yeung, Kara] Stanford Univ, Dept Biol, Stanford, CA 94305 USA. [Oster, Marina; Field, Christopher B.] Carnegie Inst Sci, Dept Global Ecol, Stanford, CA 94305 USA. [Beck, John J.] ARS, USDA, Albany, CA 94710 USA. RP Oster, M (reprint author), Stanford Univ, Dept Biol, Stanford, CA 94305 USA. EM mrus@stanford.edu FU National Science Foundation [091817]; A. W. Mellon Foundation; Carnegie Institution for Science and Stanford University FX We would like to express gratitude to Nona Chiariello of Jasper Ridge Biological Preserve at Stanford University, and Michael Dini and Todd Tobeck of the Carnegie Institution for Science for providing field advice and support. We also thank Wai Gee of the USDA Agricultural Research Service for providing technical knowledge. This Jasper Ridge Global Change experiment was supported by the National Science Foundation (Grant 091817), A. W. Mellon Foundation, Carnegie Institution for Science, and Stanford University. NR 97 TC 1 Z9 1 U1 8 U2 37 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0937-7409 EI 1423-0445 J9 CHEMOECOLOGY JI Chemoecology PD DEC PY 2015 VL 25 IS 6 BP 313 EP 323 DI 10.1007/s00049-015-0200-y PG 11 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CT5WT UT WOS:000362882500004 ER PT J AU Rehfeldt, GE Worrall, JJ Marchetti, SB Crookston, NL AF Rehfeldt, Gerald E. Worrall, James J. Marchetti, Suzanne B. Crookston, Nicholas L. TI Adapting forest management to climate change using bioclimate models with topographic drivers SO FORESTRY LA English DT Article ID POPULUS-TREMULOIDES; SEEDLING ESTABLISHMENT; COLORADO; USA; DISTRIBUTIONS; LANDSCAPE; MORTALITY; RESPONSES; DYNAMICS; ECOLOGY AB Bioclimate models incorporating topographic predictors as surrogates for microclimate effects are developed for Populus tremuloides and Picea engelmannii to provide the fine-grained specificity to local terrain required for adapting management of three Colorado (USA) national forests (1.28 million ha) and their periphery to climate change. Models were built with the Random Forests classification tree using presence-absence observations obtained by overlaying species distribution maps on data points gridded at similar to 225 m within the forests and from ground plot observations from adjacent areas. Topographic effects derived from 90-m elevation grids were expressed by weighting aspect by slope angle. Climate estimates were obtained from spline surfaces. Out-of-bag errors were similar to 17 per cent, and classification errors for an independent sample from within the forest were similar to 13 per cent. Topographic variables were second in importance to climate variables for predicting species distributions; their inclusion captured well-known topographic effects on vegetation in mountainous terrain. Predictions made for future climates described by three General Circulation Models and three emissions scenarios were used to map on 90-m grids the habitat expected to be lost, threatened, persistent or emergent. The habitat categories are used to identify those areas where treatments should have highest likelihood of success. C1 [Rehfeldt, Gerald E.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Worrall, James J.; Marchetti, Suzanne B.] US Forest Serv, Gunnison, CO 81230 USA. [Crookston, Nicholas L.] Forestry Res Consultant, Moscow, ID 83843 USA. RP Rehfeldt, GE (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. EM jrehfeldt@gmail.com NR 49 TC 1 Z9 1 U1 2 U2 36 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD DEC PY 2015 VL 88 IS 5 BP 528 EP 539 DI 10.1093/forestry/cpv019 PG 12 WC Forestry SC Forestry GA CT2XP UT WOS:000362669500003 ER PT J AU Massie, BJ Sanders, TH Dean, LL AF Massie, B. J. Sanders, T. H. Dean, L. L. TI REMOVAL OF HEAVY METAL CONTAMINATION FROM PEANUT SKIN EXTRACTS BY WASTE BIOMASS ADSORPTION SO JOURNAL OF FOOD PROCESS ENGINEERING LA English DT Article ID HULL PELLETS; EQUILIBRIUM; CHITOSAN; CAPACITY; CU(II); BEADS; IONS; HUSK AB Peanut skins are a processing waste product with potential as a low-cost source of polyphenols for nutraceutical or functional food ingredient use. Aqueous extractions of peanut skins and subsequent concentration of these extracts can result in normally innocuous levels of heavy metals present to be increased to concentrations of human health concern. Adsorption utilizing waste biomasses is a promising method of removing these contaminants from extracts. Peanut hulls and chitosan cross-linked beads were evaluated as possible adsorbents. The Langmuir adsorption model determined that peanut hulls were the more effective material. Peanut hulls removed 88.6 +/- 1.9% of the cadmium present. Apparent removal of arsenic (21.7 +/- 9.5%) showed no correlation to adsorbent dosage. Successful removal of cadmium without reduction of the phenolic content of the extracts showed that this strategy is effective for heavy metal remediation of peanut skin extracts, making them a viable source of antioxidants in food applications. C1 [Massie, B. J.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Sanders, T. H.; Dean, L. L.] ARS, Market Qual & Handling Res Unit, USDA, Raleigh, NC 27606 USA. RP Dean, LL (reprint author), ARS, Market Qual & Handling Res Unit, USDA, Raleigh, NC 27606 USA. EM lisa.dean@ars.usda.gov NR 22 TC 0 Z9 1 U1 5 U2 71 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8876 EI 1745-4530 J9 J FOOD PROCESS ENG JI J. Food Process Eng. PD DEC PY 2015 VL 38 IS 6 BP 555 EP 561 DI 10.1111/jfpe.12185 PG 7 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA CT6ZK UT WOS:000362962400004 ER PT J AU Calero-Bernal, R Verma, SK Cerqueira-Cezar, CK Schafer, LM Van Wilpe, E Dubey, JP AF Calero-Bernal, Rafael Verma, Shiv K. Cerqueira-Cezar, Camila K. Schafer, Laurence M. Van Wilpe, Erna Dubey, Jitender P. TI Sarcocystis mehlhorni, n. sp (Apicomplexa: Sarcocystidae) from the black-tailed deer (Odocoileus hemionus columbianus) SO PARASITOLOGY RESEARCH LA English DT Article DE Black-tailed deer; Odocoileus hemionus columbianus; Sarcocystis; Ultrastructure; Phylogeny ID REINDEER RANGIFER-TARANDUS; MULE DEER; SKELETAL-MUSCLE; HEMIONILATRANTIS; ULTRASTRUCTURE; VIRGINIANUS; PREVALENCE; MONTANA; COYOTES; NORWAY AB Infection with Sarcocystis is common in many species of wild cervids but none is reported from the black-tailed deer (Odocoileus hemionus columbianus). Here, we report Sarcocystis infection in two black-tailed deer from northwest USA for the first time. Sarcocysts were microscopic, up to 556 mu m long and mature. The sarcocyst wall was up to 1.39 mu m thick and had rectangular 1.17-mu m-long villar protrusions, type 17, with thin (230 nm) electron dense ground substance layer. Molecular characterization and phylogenetic analysis indicated that Sarcocystis in the black-tailed deer is related to structurally distinct Sarcocystis species in cervids. A new name, Sarcocystis mehlhorni, is proposed for the Sarcocystis species in black-tailed deer. C1 [Calero-Bernal, Rafael; Verma, Shiv K.; Cerqueira-Cezar, Camila K.; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Schafer, Laurence M.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Olympia, WA 98502 USA. [Van Wilpe, Erna] Univ Pretoria, Fac Vet Sci, Dept Anat & Physiol, ZA-0110 Onderstepoort, South Africa. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 22 TC 3 Z9 3 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD DEC PY 2015 VL 114 IS 12 BP 4397 EP 4403 DI 10.1007/s00436-015-4679-5 PG 7 WC Parasitology SC Parasitology GA CT6BM UT WOS:000362895700006 PM 26346449 ER PT J AU Staggs, SE Keely, SP Ware, MW Schable, N See, MJ Gregorio, D Zou, X Su, CL Dubey, JP Villegas, EN AF Staggs, Sarah E. Keely, Scott P. Ware, Michael W. Schable, Nancy See, Mary Jean Gregorio, Dominic Zou, Xuan Su, Chunlei Dubey, J. P. Villegas, Eric N. TI The development and implementation of a method using blue mussels (Mytilus spp.) as biosentinels of Cryptosporidium spp. and Toxoplasma gondii contamination in marine aquatic environments SO PARASITOLOGY RESEARCH LA English DT Article DE Biosentinel; Cryptosporidium; Toxoplasma gondii; Water quality; Mytilus spp. ID ENHYDRA-LUTRIS-NEREIS; CRASSOSTREA-VIRGINICA; UNITED-STATES; SEA OTTERS; DREISSENA-POLYMORPHA; GENOME AMPLIFICATION; CHESAPEAKE BAY; WATER; OOCYSTS; PARVUM AB Surveillance monitoring for microbial water quality typically involves collecting single discrete grab samples for analyzing only one contaminant. While informative, current approaches suffer from poor recoveries and only provide a limited snapshot of the microbial contaminants only at the time of collection. To overcome these limitations, bivalves have been proposed as effective biosentinels of water quality particularly for their ability to efficiently concentrate and retain microbial contaminants for long periods of time. In this study, we examined the use of indigenous blue mussels (Mytilus spp.) as biosentinels to monitor for the presence of Toxoplasma gondii and Cryptosporidium water. An efficient method to extract oocyst DNA from various mussel tissues followed by PCR-based detection of these pathogens was developed, which resulted in the detection down to 10 oocysts. This method was then used to conduct a small survey in Point Lobos and Morro Bay, California to determine prevalence T. gondii and Cryptosporidium. Results revealed that mussels from Morro Bay were contaminated with T. gondii (33 %), while mussels from Point Lobos were contaminated with T. gondii (54 %) and Cryptosporidium (26.9 %) oocysts. Phylogenetic analysis using the SSU rRNA gene identified two novel Cryptosporidium parvum-like genotypes. Overall, this study demonstrated the application of using native California Mytilus spp. as biosentinels for pathogen contamination along the central California shorelines. More importantly, T. gondii and Cryptosporidium were found at higher prevalence rates in Morro Bay and in Point Lobos, an area not previously reported to be contaminated with these pathogens. C1 [Staggs, Sarah E.; Keely, Scott P.; Ware, Michael W.; Villegas, Eric N.] US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Schable, Nancy; See, Mary Jean] Dynamac Corp, Cincinnati, OH 45268 USA. [Gregorio, Dominic] State Water Resources Control Board, Sacramento, CA 95814 USA. [Zou, Xuan; Su, Chunlei] Univ Tennessee, Div Biol, Knoxville, TN 37996 USA. [Dubey, J. P.] USDA, Anim Res Serv, Beltsville, MD 20705 USA. [Villegas, Eric N.] US EPA, Microbiol & Chem Exposure Assessment Res Div, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. RP Villegas, EN (reprint author), US EPA, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. EM villegas.eric@epa.gov RI Villegas, Eric/A-7373-2015 OI Villegas, Eric/0000-0002-8059-8588 FU United States Environmental Protection Agency through its Office of Research and Development FX We would like to thank Jim Lazorchak and Ken Fritz for technical guidance and Nichole Brinkman and Shannon Griffin for technical review. The United States Environmental Protection Agency through its Office of Research and Development funded and managed the research described here. It has been subjected to the agency's administrative review and approved for publication. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. This work was conducted, in part, under a USEPA Region 3 Regional Methods Initiative Project. SES is an Oak Ridge Institute for Science and Education postdoctoral fellow. NR 44 TC 3 Z9 3 U1 1 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD DEC PY 2015 VL 114 IS 12 BP 4655 EP 4667 DI 10.1007/s00436-015-4711-9 PG 13 WC Parasitology SC Parasitology GA CT6BM UT WOS:000362895700033 PM 26358104 ER PT J AU Wang, Y Bai, JH Long, LE AF Wang, Yan Bai, Jinhe Long, Lynn E. TI Quality and physiological responses of two late-season sweet cherry cultivars 'Lapins' and 'Skeena' to modified atmosphere packaging (MAP) during simulated long distance ocean shipping SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Prunus avium L; MAP; Flavor loss; Off-flavor; Skin darkening; Postharvest disorders; Lipid peroxidation ID PRUNUS-AVIUM L.; COLD-STORAGE; LIPID-PEROXIDATION; CARBON-DIOXIDE; FRUIT-QUALITY; BING CHERRIES; TEMPERATURE; ANTHOCYANIN; ATTRIBUTES; METABOLISM AB The production and export of late season sweet cherry cultivars continues to increase in the US Pacific Northwest (PNW). Major postharvest quality deterioration during long distance ocean shipping include flavor loss, off-flavor development, skin darkening, pedicel browning, pitting, and decay. In this research, three modified atmosphere packaging (MAP) liners with varied gas permeability were evaluated for their effect on quality deterioration and physiological changes of 'Lapins' and 'Skeena' during a simulated transit of 6 weeks at 0 degrees C. Results showed that MAP2 (O-2 6.5-7.5%, CO2 8.0-10.0%) reduced ascorbic acid (AsA) loss and lipid peroxidation, maintained flavor by retarding titratable acid loss and bitter taste formation, and kept brighter color by retarding anthocyanin synthesis compared to the macro-perforated polyethylene liner after 4 and 6 weeks. In contrast, MAP1 (O-2 12.0-13.5%, CO2 5.0-7.0%) had little benefit on maintaining fruit flavor and skin color. MAP3 (O-2 0.5-1.5%, CO2 similar to 10%), on the other hand, showed greater benefits in most of the quality attributes; however, fruit exhibited anaerobic off-flavor from a significant accumulation of ethanol, especially in 'Skeena'. All three MAP liners reduced pedicel browning and decay but did not affect pitting and splitting. In conclusion, only the MAP with the most appropriate gas permeability, which maintained O-2 6.5-7.5% and CO2 8.0-10.0%, slowed down fruit senescence and maintained quality with respect to flavor and skin color of the late season sweet cherry cultivars after long distance ocean shipping. (C) 2015 Elsevier B.V. All rights reserved. C1 [Wang, Yan] Oregon State Univ, Dept Hort, Midcolumbia Agr Res & Extens Ctr, Hood River, OR 97031 USA. [Bai, Jinhe] ARS, USDA, Hort Res Lab, Ft Pierce, FL 34945 USA. [Long, Lynn E.] Oregon State Univ Extens, The Dalles, OR 97058 USA. RP Wang, Y (reprint author), Oregon State Univ, Dept Hort, Midcolumbia Agr Res & Extens Ctr, 3005 Expt Stn Dr, Hood River, OR 97031 USA. EM yan.wang@oregonstate.edu FU Oregon Sweet Cherry Commission FX We are grateful to the Oregon Sweet Cherry Commission for their financial support of this research. NR 43 TC 0 Z9 1 U1 6 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 1 EP 8 DI 10.1016/j.postharvbio.2015.07.009 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300001 ER PT J AU Dong, TT Shi, JY Jiang, CZ Feng, YY Cao, Y Wang, QG AF Dong, Tiantian Shi, Jingying Jiang, Cai-Zhong Feng, Yanyan Cao, Yu Wang, Qingguo TI A short-term carbon dioxide treatment inhibits the browning of fresh-cut burdock SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Burdock; Fresh-cut; Carbon dioxide; Browning ID PHENYLALANINE AMMONIA-LYASE; ANTIOXIDANT ACTIVITIES; POLYPHENOL OXIDASE; LIPID-PEROXIDATION; VEGETABLES; LETTUCE; QUALITY; FRUITS; ACCUMULATION; PURIFICATION AB Fresh-cut burdock is susceptible to browning. The effect of short-term carbon dioxide (CO2) treatment on inhibiting browning of fresh-cut burdock during storage at 2-4 degrees C was investigated. The results showed that the burdock slices treated with CO2 for 4 h, 6 h and 8 h exhibited better visual quality during 8 d storage, compared with the ones treated with air. CO2 treatment for 6 h on the fresh-cut burdock slices reduced the respiration rate, lowered the activity of PPO and PAL, and the content of total phenolic compound's. On the other hand, CO2 treatment increased the content of H2O2, enhanced the activity of CAT, POD, and SOD, maintained DPPH inhibition rate and decreased the content of MDA. The results indicate that the short-term pure CO2 treatment can extend the shelf life of fresh-cut burdock by inhibiting its browning and improving its quality. (C) 2015 Elsevier B.V. All rights reserved. C1 [Dong, Tiantian; Shi, Jingying; Feng, Yanyan; Cao, Yu; Wang, Qingguo] Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China. [Jiang, Cai-Zhong] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. RP Wang, QG (reprint author), Shandong Agr Univ, Coll Food Sci & Engn, Room 304,Bldg 9,61 Daizong St, Tai An 271018, Shandong, Peoples R China. EM wqgyyy@126.com FU Shandong Provincial Research Center for Engineering and Technology of Food Safety of Fruits and Vegetables, Jinan, China FX This work was partially supported by Shandong Provincial Research Center for Engineering and Technology of Food Safety of Fruits and Vegetables, Jinan, China. NR 49 TC 1 Z9 4 U1 3 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 96 EP 102 DI 10.1016/j.postharvbio.2015.07.014 PG 7 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300013 ER PT J AU Xu, R Takeda, F Krewer, G Li, CY AF Xu, Rui Takeda, Fumiomi Krewer, Gerard Li, Changying TI Measure of mechanical impacts in commercial blueberry packing lines and potential damage to blueberry fruit SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Packing line; Blueberry; Instrumented sphere; Impact recording device; Bruising; Mechanical damage; Vaccinium ID INSTRUMENTED SPHERE; PUNCTURE INJURY; ONIONS; ROTARY AB Blueberry fruit is susceptible to bruising from mechanical impact. Bruised fruit has shorter postharvest shelf life and softens rapidly in cold storage than non-bruised fruit. A blueberry packing line consists of a hopper for transferring fruit in field containers onto a conveyor line that moves fruit into trash removal equipment, electronic sorter, inspection line, and finally onto clamshell-filling equipment. Blueberry fruit drops as it is transferred from one equipment to the next on the packing line. The mechanical impacts that occur on blueberry packing line equipment were measured quantitatively with a miniature, instrumented sphere called the blueberry impact recording device (BIRD) at 11 packing houses in the United States in 2013 and 2014. The BIRD sensor recorded impacts at transfer points or wherever there was a vertical drop on the packing line. The potential for impact damage was determined in four cultivars ('Farthing', 'O'Neal', 'Reveille' and 'Star') by dropping fruit from different heights. The measured data revealed that the largest impacts (similar to 230 g) were recorded when the sensor dropped into the hopper above the clamshell filler on eight empty lines. The cumulative peakG data showed strong correlation with overall drop height, indicating that reducing the overall drop height on a packing line could reduce the impact level. When the transfer points were padded with Poron foam sheet, significantly lower levels of impact were recorded by the sensor. The BIRD sensor also recorded lower impacts when it was run with fruit on the packing line. The severity of bruise damage resulting from fruit being dropped was related to the impact data recorded by the BIRD sensor. Using peakG-velocity change plot and the fruit bruising rate, several large impacts sufficient to cause bruising were identified, (e.g., >20% of cut surface area indicating bruise damage in 76% of 'Reveille' fruit). This paper quantitatively measured the mechanical impact on blueberry packing lines for the first time and the information will assist in improving the design and configuration of blueberry packing line equipment. These changes should result in reducing the magnitude and frequency of mechanical impacts and bruise damage in blueberry fruit. (C) 2015 Elsevier B.V. All rights reserved. C1 [Xu, Rui; Li, Changying] Univ Georgia, Coll Engn, Athens, GA 30602 USA. [Takeda, Fumiomi] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV 25430 USA. [Krewer, Gerard] Univ Georgia, Woodbine, GA 31569 USA. RP Li, CY (reprint author), Univ Georgia, Coll Engn, 200 DW Brooks Dr, Athens, GA 30602 USA. EM cyli@uga.edu FU United States Highbush Blueberry Council; USDA National Institute for Food and Agriculture Specialty Crops Research Initiative [2014-51181-22383] FX The authors would like to acknowledge funding support from United States Highbush Blueberry Council and USDA National Institute for Food and Agriculture Specialty Crops Research Initiative (Award No: 2014-51181-22383). We also thank Mr. W. O. Cline from North Carolina State University and Ms. Ann Rose from USDA ARS for their assistance in data collection and analysis. NR 27 TC 2 Z9 2 U1 7 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 103 EP 113 DI 10.1016/j.postharvbio.2015.07.013 PG 11 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300014 ER PT J AU de Frias, JA Luo, YG Kou, LP Zhou, B Wang, Q AF de Frias, J. Atilio Luo, Yaguang Kou, Liping Zhou, Bin Wang, Qin TI Improving spinach quality and reducing energy costs by retrofitting retail open refrigerated cases with doors SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Open refrigerated display cases; Display cases with doors; Baby spinach quality; Energy savings ID ESCHERICHIA-COLI O157H7; TABLE GRAPES; TEMPERATURE; DISPLAY; COMBINATION; CABINETS; STORAGE; TIME AB The prevalence of open-refrigerated display cases is ubiquitous in retail supermarkets, even in the face of the non-uniform temperature conditions present in these cases. In this paper, the temperature variations (Delta T) of packaged ready-to-eat baby spinach were evaluated for an open display case and a display case with glass doors, in order to assess the advantages of this physical barrier in minimizing Delta T and decay rate, and improving the visual quality of the samples after four days of storage. The two 3.66 m display cases were installed in the same room and conditions were constant at 21 degrees C and 60-70% of relative humidity, with a thermostat setting for both cases set at 0.6 degrees C. Results showed that the display case with doors significantly improved temperature uniformity and compliance with the U.S. Food and Drug Administration (FDA) Food Code recommendation of 5 degrees C or less to prevent microbial pathogen growth in packaged leafy greens. Only 1% of the temperature readings over four days in the case with doors were non-compliant with the FDA Food Code, while 24% of the readings in the open case were non-compliant; mostly recorded by the front positions of the case. The lower temperatures and Delta T of the case with doors were consistent with the higher visual quality scores (P < 0.001) for the baby spinach samples recorded by trained panelists, based on a 9-point hedonic scale, at 7.2 and 6.6 for the case with doors and the open case, respectively. Differences in decay rate were significant (P < 0.001) by the front of the case, with mean values of 8.8% for the open case and 5.5% for the case with doors. Furthermore, operational energy costs were 69% less than the open display case and the cost of door retrofits can be recouped in less than two years by energy savings alone. Published by Elsevier B.V. C1 [de Frias, J. Atilio; Luo, Yaguang; Zhou, Bin] ARS, USDA, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. [de Frias, J. Atilio; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. RP Luo, YG (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Food Qual Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov FU USDA-NIFA (Specialty Crops Research Initiative Award) [2010-51181-21230]; USDA-ARS [1275-43440-004-00D] FX This research was supported by USDA-NIFA (Specialty Crops Research Initiative Award No. 2010-51181-21230) and USDA-ARS (Project No. 1275-43440-004-00D). NR 23 TC 0 Z9 0 U1 9 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 114 EP 120 DI 10.1016/j.postharvbio.2015.06.016 PG 7 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300015 ER PT J AU Xiao, ZL Lester, GE Park, E Saftner, RA Luo, YG Wang, Q AF Xiao, Zhenlei Lester, Gene E. Park, Eunhee Saftner, Robert A. Luo, Yaguang Wang, Qin TI Evaluation and correlation of sensory attributes and chemical compositions of emerging fresh produce: Microgreens SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Microgreens; Consumer acceptance; Flavor; Titratable acidity; Phytonurient; Total phenolic content ID ANTIOXIDANT ACTIVITY; TOMATO FLAVOR; ASCORBIC-ACID; QUALITY; VEGETABLES; ACCUMULATION; PHENOLICS; CONSUMER; COOKING; FRUITS AB Microgreens are an emerging food product with scarce information pertaining to their sensory and nutritional properties. In this study, six species of microgreens, including Dijon mustard (Brassica juncea L. Czem.), opal basil (Ocimum basilicum L), bull's blood beet (Beta vulgaris L), red amaranth (Amaranthus tricolor L), peppercress (Lepidium bonariense L.) and China rose radish (Raphanus sativus L), were evaluated for their sensory attributes and chemical compositions. Results showed that bull's blood beet had the highest rating on acceptability of flavor and overall eating quality while peppercress the lowest. Chemical compositions also differed significantly among the six species. China rose radish had the highest titratable acidity and total sugars, while red amaranth had the highest pH value and lowest total sugars. Regarding the phytonutrient concentrations, the highest concentrations of total ascorbic acid, phylloquinone, carotenoids, tocopherols, and total phenolics were found in China rose radish, opal basil, red amaranth, China rose radish, and opal basil, respectively. The relationships between sensory-sensory attributes and sensory-chemical compositions were further studied. It was found that overall eating quality of microgreens was best correlated with flavor score and microgreen's pH value and total phenolic content were strongly correlated with flavor attributes, e.g., sourness, astringency, and bitterness. In general, despite the differences among individual microgreens, all of the microgreens evaluated in this study demonstrated "good" to "excellent" consumer acceptance and nutritional quality. (C) 2015 Elsevier B.V. All rights reserved. C1 [Xiao, Zhenlei; Lester, Gene E.; Park, Eunhee; Saftner, Robert A.; Luo, Yaguang] ARS, Food Qual Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Xiao, Zhenlei; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Wang, Q (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM gene.lester@ars.usda.gov; wangqin@umd.edu FU USDA-ARS Project [1265-43440-004-00] FX The authors would like to thank Dr. Karen Bret-Garber for critical reading and valuable suggestions of the manuscript. The authors are also very thankful to the donation of microgreens by Fresh Origins, LLC (San Marcos, CA, USA) and the participation of our sensory panel. Use of a company or product name by the U.S. Department of Agriculture does not imply approval or recommendation of the product to the exclusion of others that may also be suitable. This study was supported by USDA-ARS Project 1265-43440-004-00. NR 25 TC 1 Z9 1 U1 6 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 140 EP 148 DI 10.1016/j.postharvbio.2015.07.021 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300018 ER PT J AU Arpaia, ML Collin, S Sievert, J Obenland, D AF Arpaia, Mary Lu Collin, Sue Sievert, James Obenland, David TI Influence of cold storage prior to and after ripening on quality factors and sensory attributes of 'Hass' avocados SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Sensory; Hedonic score; Maturity; Ripening ID MESOCARP DISCOLORATION; FRUIT; TEMPERATURES; MATURITY; INJURY AB Partially-ripened avocados are often held in cold storage in an attempt to enable the consistent delivery of ripe fruit to food service or retail outlets. It is also common to hold fruit without any prior ripening for a few days to several weeks prior to ethylene treatment and final ripening. The purpose of this study was to determine the impact of these practices on ripening time and subsequent fruit quality. 'Hass' avocados were harvested from three different orchards on six harvest dates and placed at either 5 degrees C or 12 degrees C immediately after harvest or after ripening to average firmness values of either 80 N (partially ripe) or 19 N (near ripe). After storage the fruit were then ripened to eating firmness (4.4-6.7 N) and evaluated for quality parameters. A portion of the fruit at harvest firmness were stored for 0 d, 7 d, 14 d or 28 d and then treated with ethylene to ripen the fruit. Continual softening during storage resulted in fruit that had been ripened prior to storage being nearly at eating firmness at the end of 14 d, especially those stored at 12 degrees C. Stem end rot, body rot and pink staining of the vascular tissue occurred more frequently in the previously-ripened fruit after 14 d, the severity being greatest at 12 degrees C where the incidence exceeded 50% for both stem end rot and pink staining. Both harvest date and orchard influenced the amount of pink staining observed. Storage of firm fruit prior to ethylene treatment resulted in higher levels of both stem end rot and pink staining only when the storage time exceeded 14 d. In an additional study to assess the impact of the storage of partially-ripened fruit on both quality and sensory characteristics, 'Hass' avocados were harvested six times at monthly intervals, ripened to approximately 16 N firmness at 20 degrees C and then placed at either 1 degrees C or 5 degrees C for 7 d or 14 d. Following storage the fruit were ripened to eating firmness and evaluated. The occurrence of fruit quality defects was not affected by the timing of ripening. Sensory panelists liked fruit ripened prior to or after cold storage equally well and there were no differences among the ripening treatments, within a storage time, in the level of rich, nutty or grassy attributes that composed the fruit flavor. There were statistically-significant differences in the degree of textural creaminess among the ripening treatments, although these differences were inconsistent and slight. Storing partially ripe 'Hass' avocados for up to 4 d at either 1 degrees C, 5 degrees C or 12 degrees C does not greatly alter avocado quality, while storage for longer periods, such as 14 cl, may be problematic due to enhanced development of decay and pink staining, especially at higher temperatures. Holding unripe avocados for longer than 14 d prior to ethylene treatment may predispose the fruit to the development of pink staining. Published by Elsevier B.V. C1 [Arpaia, Mary Lu; Collin, Sue; Sievert, James] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Obenland, David] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Obenland, D (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM david.obenland@ars.usda.gov FU California Avocado Commission FX This work was partially-funded by a grant from the California Avocado Commission. Thanks to Paul Neipp and Kent Fjeld for their assistance in conducting this project. Thanks also to Victor Tokar and Reuben Hofshi for their practical advice on commercial handling practices for avocado and for suggesting this research topic. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer NR 20 TC 0 Z9 0 U1 7 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD DEC PY 2015 VL 110 BP 149 EP 157 DI 10.1016/j.postharvbio.2015.07.016 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CT6JM UT WOS:000362919300019 ER PT J AU Cuevas, HE Prom, LK Erpelding, JE AF Cuevas, Hugo E. Prom, Louis K. Erpelding, John E. TI Tapping the US Sweet Sorghum Collection to Identify Biofuel Germplasm SO SUGAR TECH LA English DT Article DE Biofuel; Biomass; Brix; Exotic germplasm; Sorghum bicolor; Sweet sorghum ID BICOLOR L. MOENCH; GENETIC DIVERSITY; SUGAR CONTENT; JUICE; REGISTRATION; VARIETIES; MARKERS; BRIX AB The narrow genetic base in sweet sorghum [Sorghum bicolor (L.) Moench] breeding programs is limiting the development of new varieties for biofuel production. Therefore, the identification of genetically diverse sweet sorghum germplasm in the U.S. National Plant Germplasm System (NPGS) collection is imperative for biofuel breeding programs as biofuel production expands to new regions. Nine-hundred twenty-five sweet sorghum accessions from the NPGS collection were agronomically evaluated and a subset of 56 accessions selected for further evaluation. A 2 year replicated trial of this subset together with 17 U.S. sweet sorghum varieties were evaluated for agronomic and biofuel traits flowering time, plant height, fresh and dry weight, brix, juice volume, percent of moisture, and fermentable sugars [dinitrosalicylic (DNS) method] and disease response [anthracnose (Colletotricum sublineolum) and rust (Purcina purpurea)]. Nine accessions from the NPGS collection originally from South Africa, Ethiopia, Sudan, Zimbabwe, and the U.S. showed brix values ranging from 10 to 14, with five accessions having a higher amount of fermentable sugars than U.S. references accessions (DNS = 9.86-11.42). Likewise, the total dry matter content of three accessions originally from Ethiopia and U.S. were higher than the U.S. reference accessions (>156.87 g/plant). Multiple new sources of anthracnose and rust resistance were identified; being PI 156424 from Tanzania resistant to both diseases. The results demonstrated that accessions in the NPGS sorghum collection enclose valuable genes/alleles for biofuel traits that are not being used in U.S. biofuel breeding programs. Thus, the integration of these accessions into these programs will aid to increase genetic diversity and development of new biofuel varieties. C1 [Cuevas, Hugo E.; Erpelding, John E.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Prom, Louis K.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Erpelding, John E.] USDA ARS, Stoneville, MS 38776 USA. RP Cuevas, HE (reprint author), USDA ARS, Trop Agr Res Stn, 2200 Pedro Albizu Campos Ave Ste 201, Mayaguez, PR 00680 USA. EM hugo.cuevas@ars.usda.gov NR 36 TC 1 Z9 1 U1 3 U2 9 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD DEC PY 2015 VL 17 IS 4 BP 428 EP 438 DI 10.1007/s12355-014-0349-7 PG 11 WC Agronomy SC Agriculture GA CS9RU UT WOS:000362429900012 ER PT J AU Howell, TA Evett, SR Tolk, JA Copeland, KS Marek, TH AF Howell, Terry A. Evett, Steven R. Tolk, Judy A. Copeland, Karen S. Marek, Thomas H. TI Evapotranspiration, water productivity and crop coefficients for irrigated sunflower in the US Southern High Plains SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Sunflower; Evapotranspiration; Reference ET; Crop coefficient; Growing degree days; Yield; Water use efficiency; Sprinkler irrigation ID GRAIN-SORGHUM; WEIGHING LYSIMETER; DEFICIT IRRIGATION; LIMITED IRRIGATION; USE EFFICIENCY; GROWTH-STAGES; GREAT-PLAINS; FULL; YIELD; FIELD AB Sunflower is a diverse crop grown for oil or confectionary uses in the U.S. Southern High Plains, often under irrigation, but its water use, water productivity (water use efficiency) and crop coefficients for irrigation scheduling are not well known for the Texas High Plains. Crop water use (evapotranspiration or ET) was measured in 2009 and 2011 in two 4.4-ha fields using two precision 9 m(2) weighing lysimeters containing 2.3-m deep monoliths of Pullman clay loam soil. The fields were irrigated with a lateral move sprinkler system with nozzles about 1.7-1.8 m above the ground and 1.5-m apart. The sunflower ET averaged 638 mm; seed yields averaged 308 g m(-2); and the lysimeter crop water productivity averaged 0.49 kg (dry seed + hull) m(-3). Even in the 2011 record drought season with limited soil water reserves, seed yield and oil content appeared similar to those for the 2009 season with greater precipitation and less irrigation requirement. Also, a month later sowing date in 2011, which might occur following an early cotton crop failure, did not appear to greatly affect ET, crop coefficients, or sunflower seed yields. The basal crop coefficients were 0.15 for the initial period after planting (K-cbini) and 1.22 for the peak water use rate at full cover (K-cbmid) based on the daily ASCE short "grass" reference ET (ETos) and FAO 56 climate adjustment. The K-cbmid based on the ASCE taller, rougher "alfalfa" Reference ET (ETrs) was 0.80. Using a thermal-time base (growing degree day) for the crop coefficient did not greatly improve the representation of the crop coefficient. Comparisons of ASCE reference ET computed using hourly versus daily summary data, and for short and tall reference crops, to each other and to FAO 56 reference ET showed that the relationships between reference ET methods varied significantly from one year to the next. This climate effect means that conversions of crop coefficients from one standard ET formulation to another will not be straightforward. Published by Elsevier B.V. C1 [Howell, Terry A.; Evett, Steven R.; Tolk, Judy A.; Copeland, Karen S.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Marek, Thomas H.] Texas AgriLife Res, Agr Res & Extens Ctr, Amarillo, TX 79106 USA. RP Evett, SR (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM tah1@att.net; steve.evett@ars.usda.gov; judy.tolk@ars.usda.gov; karen.copeland@ars.usda.gov; t-marek@tamu.edu FU USDA-ARS FX We gratefully acknowledge support from the USDA-ARS Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University. Additionally, many Conservation and Production Research Laboratory scientists and technicians contributed to this work and their contributions are gratefully acknowledged. Dr. Gerald Seiler (ARS Fargo N.D.) provided the sunflower oil analyses. NR 61 TC 4 Z9 4 U1 5 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD DEC PY 2015 VL 162 BP 33 EP 46 DI 10.1016/j.agwat.2015.08.008 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CS5UP UT WOS:000362144100003 ER PT J AU Zhang, MP Shan, YJ Kochian, L Strasser, RJ Chen, GX AF Zhang, Meiping Shan, YongJie Kochian, Leon Strasser, Reto J. Chen, GuoXiang TI Photochemical properties in flag leaves of a super-high-yielding hybrid rice and a traditional hybrid rice (Oryza sativa L.) probed by chlorophyll a fluorescence transient SO PHOTOSYNTHESIS RESEARCH LA English DT Article DE Chlorophyll a fluorescence transient; Flag leaf; JIP-test; Hybrid rice (Oryza sativa L.); Photochemistry; Performance index ID J-I-P; CYANOBACTERIUM SPIRULINA-PLATENSIS; PHOTOSYSTEM-II; SOYBEAN GENOTYPES; PROTEIN-PHOSPHORYLATION; LIPID-PEROXIDATION; WHEAT PLANTS; HEAT-STRESS; PHOTOSYNTHESIS; SENESCENCE AB Chlorophyll a fluorescence of flag leaves in a super-high-yielding hybrid rice (Oryza sativa L.) LYPJ, and a traditional hybrid rice SY63 cultivar with lower grain yield, which were grown in the field, were investigated from emergence through senescence of flag leaves. As the flag leaf matured, there was an increasing trend in photosynthetic parameters such as quantum efficiency of primary photochemistry ( (Po)) and efficiency of electron transport from PS II to PS I (I (Eo)). The overall photosynthetic performance index (PIABS) was significantly higher in the high-yielding LYPJ compared to SY63 during the entire reproductive stage of the plant, the same to MDA content. However, (Po)(=F (V)/F (M)), an indicator of the primary photochemistry of the flag leaf, did not display significant changes with leaf age and was not significantly different between the two cultivars, suggesting that PIABS is a more sensitive parameter than (Po) (=F (V)/F (M)) during leaf age for distinguishing between cultivars differing in yield. C1 [Zhang, Meiping] ShanXi Normal Univ, Coll Life Sci, Linfen 041004, Shanxi, Peoples R China. [Shan, YongJie] ShanXi Normal Univ, Coll Geog Sci, Linfen 041004, Shanxi, Peoples R China. [Kochian, Leon] Cornell Univ, USDA ARS, Robert W Holly Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Strasser, Reto J.] Univ Geneva, Bioenerget Lab, CH-1254 Geneva, Switzerland. [Chen, GuoXiang] Nanjing Normal Univ, Coll Life Sci, Key Lab Biodivers & Biotechnol Jiangsu Prov, Nanjing 210097, Jiangsu, Peoples R China. RP Zhang, MP (reprint author), ShanXi Normal Univ, Coll Life Sci, Linfen 041004, Shanxi, Peoples R China. EM mpzhang2006@163.com; gxchen@njnu.edu.cn FU National Natural Sciences Foundation of China [31271621, 31301245]; Natural Sciences Foundation of Shanxi province [2009021030-2]; State Key Laboratory of Crop Biology of Shandong Agricultural University [2014KF03]; Shanxi Scholarship Council of China [2013-067] FX Financial support was provided by the National Natural Sciences Foundation of China (No. 31271621 and No. 31301245); Natural Sciences Foundation of Shanxi province (2009021030-2); Open project funds of State Key Laboratory of Crop Biology of Shandong Agricultural University (2014KF03); and by Shanxi Scholarship Council of China (No. 2013-067). We also want to thank Mr. Lv ChuanGen for his help with materials and Zhang ChengJun for his guide with our experiment. NR 41 TC 1 Z9 1 U1 5 U2 48 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0166-8595 EI 1573-5079 J9 PHOTOSYNTH RES JI Photosynth. Res. PD DEC PY 2015 VL 126 IS 2-3 SI SI BP 275 EP 284 DI 10.1007/s11120-015-0151-8 PG 10 WC Plant Sciences SC Plant Sciences GA CS3WU UT WOS:000362006900008 PM 25972274 ER PT J AU Franzluebbers, AJ AF Franzluebbers, Alan J. TI Farming strategies to fuel bioenergy demands and facilitate essential soil services SO GEODERMA LA English DT Article DE Biofuels; Conservation agricultural systems; Crop residues; Soil management; Soil organic carbon ID NO-TILL CORN; ORGANIC-CARBON SEQUESTRATION; CROP RESIDUE MANAGEMENT; SOUTHERN PIEDMONT USA; UNITED-STATES; SUSTAINABLE INTENSIFICATION; AGRICULTURAL SYSTEMS; NITROGEN MANAGEMENT; BIOFUEL PRODUCTION; NUTRIENT-UPTAKE AB Perennial lignocellulosic biomass and food crop residues have traditionally been important resources used internally on-farm. However given the growing outlook with advanced biofuel conversion technologies, such biomass sources might be of competing greater value if sold off the farm into the bioenergy feedstock stream. Inputs of carbon embedded in above-ground plant biomass are a key biological energy source for the soil surface a zone of great importance in the success of food-feed-fiber production and ecological processes essential to environmental quality. This review of literature looks at how above-ground plant biomass contributes to soil properties and processes, water conservation and quality, on-farm forage availability, and as a harvestable biofuel component. Competing needs for this resource could cause serious environmental or economic consequences without sufficient knowledge of their potential impacts. Perennial forages and crop residues are critical for providing surface cover to protect soils against erosion and for providing the organic inputs to support below-ground ecosystem communities, properties, and processes. The amount of biomass required to maintain soil organic matter and various ecosystem services linked to this key soil property may, in many cases, exceed that needed for simple erosion control. Consequences of continuous residue harvest could be detrimental or minor, depending on the climatic and edaphic conditions, as well the type of cropping system and tillage management employed. Achieving a balanced outcome will require scientific evidence of in-field effects, a collective vision for designing landscapes of appropriate functional capacity, and well-designed government policies for crop residue and perennial biomass utilization schemes to contribute to a sustainable agricultural approach. Published by Elsevier B.V. C1 USDA ARS, Raleigh, NC 27695 USA. RP Franzluebbers, AJ (reprint author), USDA ARS, 3218 Williams Hall,NCSU Campus Box 7619, Raleigh, NC 27695 USA. EM alan.franzluebbers@ars.usda.gov FU US Forest Service [12-IA-11330152-051] FX Thanks are extended to Mr. Steven W. Knapp, Ms. Ellen Leonard, and Ms. Erin L Silva for their technical support in lab and field studies. Recent financial support to develop a research project that spawned some of these ideas was provided by the US Forest Service (Agr. No. 12-IA-11330152-051). NR 89 TC 1 Z9 1 U1 12 U2 116 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD DEC PY 2015 VL 259 BP 251 EP 258 DI 10.1016/j.geoderma.2015.06.007 PG 8 WC Soil Science SC Agriculture GA CQ8OM UT WOS:000360867800026 ER PT J AU Sheen, S Cassidy, J Scullen, B Sommers, C AF Sheen, Shiowshuh Cassidy, Jennifer Scullen, Butch Sommers, Christopher TI Inactivation of a diverse set of shiga toxin-producing Escherichia coli in ground beef by high pressure processing SO FOOD MICROBIOLOGY LA English DT Article DE High pressure processing; Ground beef; Shiga toxin-producing E. coli; Virulence factors; Surrogates ID HIGH HYDROSTATIC-PRESSURE; FOODBORNE ILLNESS; MEAT-PRODUCTS; UNITED-STATES; O157H7; SURVIVAL; IRRADIATION; PATTIES AB Shiga toxin-producing Escherichia coli (STEC) are regularly implicated in foodborne illness outbreaks and recalls of ground beef. In this study we determined the High Pressure Processing (HPP) D-10 value (the processing conditions needed to reduce the microbial population by 1 log) of 39 STEC isolates, including the "big six" serovars, O104 and O157: H7. STEC isolates included those isolated from animals and environmental sources in addition to those associated with illness in humans. Individual STEC were inoculated into 80% lean ground beef and treated with HPP (350 MPa, 4 degrees C, up to 40 min). The mean D-10 was 9.74 min, with a range of 0.89-025.70 min. The D-10 of the STEC involved in human illness was 9.25 vs. 10.40 min for those not involved in human illness (p > 0.05). The presence or absence of genes encoding virulence factors (e.g. Shiga toxin 1 or 2, intimin, or enterohemolysin) had no effect on the HPP D-10 (p > 0.05). The high D-10 of some STEC involved in human illness should be considered in selecting HPP processing parameters for ground beef. This study demonstrates the heterogeneity of STEC resistance to HPP. Risk assessors and the food industry can use this information to provide safer meat products to consumers. Published by Elsevier Ltd. C1 [Sheen, Shiowshuh; Cassidy, Jennifer; Scullen, Butch; Sommers, Christopher] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov NR 32 TC 5 Z9 5 U1 8 U2 65 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD DEC PY 2015 VL 52 BP 84 EP 87 DI 10.1016/j.fm.2015.07.001 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CQ5OA UT WOS:000360653500010 PM 26338120 ER PT J AU Wang, W Ni, XZ Lawrence, KC Yoon, SC Heitschmidt, GW Feldner, P AF Wang, Wei Ni, Xinzhi Lawrence, Kurt C. Yoon, Seung-Chul Heitschmidt, Gerald W. Feldner, Peggy TI Feasibility of detecting Aflatoxin B-1 in single maize kernels using hyperspectral imaging SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Aflatoxin B-1 (AFB(1)); Maize; Hyperspectral imaging (HSI); Spectral angle mapper (SAM) classifier; Score image; Principal component analysis (PCA) ID NEAR-INFRARED SPECTROSCOPY; ASPERGILLUS-FLAVUS; CORN KERNELS; FUSARIUM-VERTICILLIOIDES; REFLECTANCE SPECTROSCOPY; RAPID DETECTION; TRANSMITTANCE; FLUORESCENCE; MYCOTOXINS; FUMONISIN AB The feasibility of detecting Aflatoxin B-1 (AFB(1)) in single maize kernel inoculated with Aspergillus flavus conidia in the field, as well as its spatial distribution in the kernels, was assessed using near-infrared hyperspectral imaging (HSI) technique. Firstly, an image mask was applied to a pixel-based image mosaic to remove background and shading. Secondly, bad lines in spectra imaging caused by inherent defects of Mercury Cadmium Telluride (MCT) detector were removed through an interactive analysis based on principal component analysis (PCA). Then a PCA procedure was carried out again on the cleaned image, key wavelengths such as 1729 and 2344 nm were shown clearly from the loading line plot of the seventh principal component (PC7). And the pixel of AFB(1) extracted from the 5-dimensional scatter plot space formed by five principal components (PCs) from PC4 to PC8 (especially PC7 and PC5) were taken as the input of the spectral angle mapper (SAM) classifier, accuracies of the three varieties of kernels reached 96.15%, 80%, and 82.61% respectively if kernels containing either high (>= 100 ppb) or low (<10 ppb) levels of aflatoxin. A slightly better test result could be got if the kernels placed with different germ orientation. Finally, the repeatability was verified using the fourth variety of kernels. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Wang, Wei] China Agr Univ, Coll Engn, Beijing Key Lab Optimized Design Modern Agr Equip, Beijing 100083, Peoples R China. [Ni, Xinzhi] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Lawrence, Kurt C.; Yoon, Seung-Chul; Heitschmidt, Gerald W.; Feldner, Peggy] USDA ARS, Qual & Safety Assessment Res Unit, Richard B Russell Res Ctr, Athens, GA 30605 USA. RP Wang, W (reprint author), China Agr Univ, Coll Engn, Beijing Key Lab Optimized Design Modern Agr Equip, 17 Tsinghua East Rd, Beijing 100083, Peoples R China. EM playerwxw@cau.edu.cn RI User, Test/G-7821-2015 FU China National Science and Technology Support Program [2012BAK08B04] FX The authors would like to express their gratitude to Vernon Savage, engineering Technician in the Quality & Safety Assessment Research Unit, USDA-ARS, Athens, GA, for his technical assistance in fabricating the sample holders; and to Penny J Tapp and Trevor Perla in the Crop Genetics and Breeding Research Unit, USDA-ARS, Tifton, GA, for inoculation and maize sample collection, and aflatoxin analysis. This work was supported financially by the China National Science and Technology Support Program (2012BAK08B04). NR 35 TC 5 Z9 6 U1 8 U2 67 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD DEC PY 2015 VL 166 BP 182 EP 192 DI 10.1016/j.jfoodeng.2015.06.009 PG 11 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA CO7IB UT WOS:000359331700021 ER PT J AU Geveke, DJ Aubuchon, I Zhang, HQ Boyd, G Sites, JE Bigley, ABW AF Geveke, David J. Aubuchon, Isolde Zhang, Howard Q. Boyd, Glenn Sites, Joseph E. Bigley, Andrew B. W. TI Validation of a pulsed electric field process to pasteurize strawberry puree SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Validation; Data acquisition; Pasteurization; Strawberry pure; Pulsed electric field ID ESCHERICHIA-COLI O157H7; JUICE; INACTIVATION; STABILITY; ANTHOCYANINS AB An inexpensive data acquisition method was developed to validate the exact number and shape of the pulses applied during pulsed electric field (PEF) processing. The novel validation method was evaluated in conjunction with developing a PEF process for pasteurizing strawberry puree. Both buffered peptone water (BPW) and fresh strawberry puree (pH 2.4) were inoculated with Escherichia coli (ATCC 35218) and processed using a pilot plant PEF system at field strengths of 24.0-33.6 kV/cm, outlet temperatures of 45.0-57.5 degrees C and a flow rate of 1001/hr. An accelerated aging storage study was performed on the quality of a strawberry beverage made from the strawberry puree. The populations of E. cob were reduced by 6.5 log in BPW at 30 kV/cm and 57.5 degrees C and 7.3 log in strawberry puree at 24 kV/cm and 52.5 degrees C. The taste and color of strawberry beverage initially made from PEF processed puree was fresh and bright red, respectively. The color remained good for the first 3 months-equivalent of storage and there was only a very slight drop in flavor. The data acquisition system captured the details of every pulse applied at a rate of 400 Hz for 1 h for a total of over 1.4 million pulses. Strawberry puree was pasteurized in a pilot-scale PEF unit. In addition, a data acquisition system was developed to validate the process which should aid in obtaining FDA approval of the PEF process. Published by Elsevier Ltd. C1 [Geveke, David J.; Boyd, Glenn; Sites, Joseph E.; Bigley, Andrew B. W.] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Aubuchon, Isolde] Amer Beverage Marketers, New Albany, IN 47150 USA. [Zhang, Howard Q.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Geveke, DJ (reprint author), ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.geveke@ars.usda.gov NR 21 TC 0 Z9 0 U1 10 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD DEC PY 2015 VL 166 BP 384 EP 389 DI 10.1016/j.jfoodeng.2015.05.008 PG 6 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA CO7IB UT WOS:000359331700045 ER PT J AU Berthe, SCF Derocles, SAP Lunt, DH Kimball, BA Evans, DM AF Berthe, Sophie C. F. Derocles, Stephane A. P. Lunt, David H. Kimball, Bruce A. Evans, Darren M. TI Simulated climate-warming increases Coleoptera activity-densities and reduces community diversity in a cereal crop SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Global warming; Climate change; Carabidae; Biocontrol; Agro-ecosystem; Ecosystem services ID GROUND BEETLES COLEOPTERA; TERRESTRIAL ECOSYSTEMS; GLOBAL CHANGE; TEMPERATURE; MANIPULATION; ARTHROPODS; CARABIDAE; VOLTINISM; MODELS; PESTS AB Simulated climate-warming experiments have provided important insights into the response of terrestrial ecosystems, but few have examined the impacts on agricultural insects, particularly those associated with the ecosystem service of biological pest control. Within a spring-sown wheat crop, we artificially increased temperature by 2 degrees C and precipitation by 10% in a short-term (April to August 2013) replicated open-field experiment and examined the impacts on coleopteran (mainly Carabidae) diversity and 'activity-densities'. Diversity indices decreased as a result of warming but were not affected by extra precipitation. We found a significant increase in activity-densities of the four most trapped species due to warming, which was responsible for observed changes in diversity. However, Staphylinidae beetles were negatively affected by the warming treatments while other, less common species were not affected. We provide the first experimental evidence of climate-driven impacts on an important farmland insect community. We discuss the implications of our results in the context of biological control and top-down effects across trophic levels. (C) 2015 Elsevier B.V. All rights reserved. C1 [Berthe, Sophie C. F.; Derocles, Stephane A. P.; Lunt, David H.; Evans, Darren M.] Univ Hull, Sch Biol Biomed & Environm Sci, Kingston Upon Hull HU6 7RX, N Humberside, England. [Kimball, Bruce A.] ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. RP Derocles, SAP (reprint author), Univ Hull, Sch Biol Biomed & Environm Sci, Kingston Upon Hull HU6 7RX, N Humberside, England. EM berthe.sophie@gmail.com; s.derocles@hull.ac.uk; d.h.lunt@hull.ac.uk; Bruce.Kimball@ARS.USDA.GOV; d.evans@hull.ac.uk OI Lunt, David H/0000-0002-9000-7470; Evans, Darren/0000-0003-4061-6726 FU University of Hull; Higher Education Innovation Fund (UK) FX The project was funded by the University of Hull, with support from The Higher Education Innovation Fund (UK). We thank staff at Stockbridge Technology Centre for help and support. NR 29 TC 1 Z9 1 U1 6 U2 304 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD DEC 1 PY 2015 VL 210 BP 11 EP 14 DI 10.1016/j.agee.2015.05.001 PG 4 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA CL5JT UT WOS:000356996900002 ER PT J AU Galant, AL Kaufman, RC Wilson, JD AF Galant, A. L. Kaufman, R. C. Wilson, J. D. TI Glucose: Detection and analysis SO FOOD CHEMISTRY LA English DT Review DE Glucose; Glucose meters; Iodometry; Detection; Quantification ID PERFORMANCE LIQUID-CHROMATOGRAPHY; ANION-EXCHANGE CHROMATOGRAPHY; PULSED AMPEROMETRIC DETECTION; SITE-DIRECTED MUTAGENESIS; DIRECT UV DETECTION; CAPILLARY-ELECTROPHORESIS; SUBSTRATE-SPECIFICITY; CARBOHYDRATE ANALYSIS; ENZYMATIC METHOD; REDUCING SUGARS AB Glucose is an aldosic monosaccharide that is centrally entrenched in the processes of photosynthesis and respiration, serving as an energy reserve and metabolic fuel in most organisms. As both a monomer and as part of more complex structures such as polysaccharides and glucosides, glucose also plays a major role in modern food products, particularly where flavor and or structure are concerned. Over the years, many diverse methods for detecting and quantifying glucose have been developed; this review presents an overview of the most widely employed and historically significant, including copper iodometry, HPLC, GC, CZE, and enzyme based systems such as glucose meters. The relative strengths and limitations of each method are evaluated, and examples of their recent application in the realm of food chemistry are discussed. Published by Elsevier Ltd. C1 [Galant, A. L.; Kaufman, R. C.; Wilson, J. D.] ARS, USDA, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. RP Wilson, JD (reprint author), ARS, USDA, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA. EM jeff.d.wilson@ars.usda.gov NR 80 TC 11 Z9 11 U1 40 U2 414 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD DEC 1 PY 2015 VL 188 BP 149 EP 160 DI 10.1016/j.foodchem.2015.04.071 PG 12 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CK9GP UT WOS:000356548900022 PM 26041177 ER PT J AU Dubey, JP Lehmann, T Lautner, F Kwok, OCH Gamble, HR AF Dubey, J. P. Lehmann, T. Lautner, F. Kwok, O. C. H. Gamble, H. R. TI Toxoplasmosis in sentinel chickens (Gallus domesticus) in New England farms: Seroconversion, distribution of tissue cysts in brain, heart, and skeletal muscle by bioassay in mice and cats SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Chickens; Gallus domesticus; Seroconversion; Tissue cyst distribution ID GONDII INFECTION; OOCYSTS; STATES AB Free-range chickens are a good indicator of soil contamination with oocysts because they feed from the ground and they are also an important source of infection for cats that in turn shed oocysts after eating tissues of intermediate hosts. Little is known of the epidemiology of toxoplasmosis in chickens. In the present study 90 Toxoplasma gondii seronegative, sentinel chickens were placed on three (30 each) swine farms in New England in November, 2003. Chickens were bled monthly and their sera were tested for T. gondii antibodies by the modified agglutination test (MAT, cut-off 1:25). Chickens that seroconverted were euthanized and their tissues were bioassayed in mice, cats, or both. Over the course of the experiment (7 months), 31 of 71 chickens seroconverted (MAT 1:100 or higher). Tissues of 26 seropositive chickens were bioassayed in both cats and mice; viable T. gondii was isolated, by bioassay in mice, from hearts (whole) of all 26 chickens, brains (whole) of 3 chickens and leg muscles (25 g) of 11 chickens; 21 of 26 cats fed 250 g of muscle from seropositive chickens excreted T. gondii oocysts. Results indicated that the density of T. gondii in poultry muscle is low but heart is the tissue of choice for isolation of viable parasites. Published by Elsevier B.V. C1 [Dubey, J. P.; Kwok, O. C. H.] ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Lehmann, T.] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. [Gamble, H. R.] Natl Acad Sci, Washington, DC 20001 USA. RP Dubey, JP (reprint author), ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov FU Food Safety Initiative, Center for Diseases Control (CDC), Atlanta, Georgia FX This research was supported, in part, by the Food Safety Initiative, Center for Diseases Control (CDC), Atlanta, Georgia. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the Department of Health and Human Services or the Centers for Disease Control and Prevention or the U. S. Department of Agriculture. NR 12 TC 3 Z9 3 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD NOV 30 PY 2015 VL 214 IS 1-2 BP 55 EP 58 DI 10.1016/j.vetpar.2015.09.004 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CY9ZO UT WOS:000366765100009 PM 26391819 ER PT J AU Cole, JB AF Cole, John B. TI A simple strategy for managing many recessive disorders in a dairy cattle breeding program SO GENETICS SELECTION EVOLUTION LA English DT Article ID MATE SELECTION; HOLSTEIN; GENETICS; GENES AB Background: High-density single nucleotide polymorphism genotypes have recently been used to identify a number of novel recessive mutations that adversely affect fertility in dairy cattle, as well as to track other conditions such as red coat color and polled. Most current methods for mate allocation fail to consider this information, and it will become increasingly difficult to manage matings as the number of recessive mutations to be accounted for increases. Methods: A modified version of a mating strategy that constrains inbreeding based on genomics (the Pryce method) was developed that also accounts for the economic effects of Mendelian disorders on overall economic merit (modified Pryce method) and compared with random mating, truncation selection, and the Pryce scheme. Several scenarios were considered, including scenarios with six hypothetical recessive alleles and 12 recessive alleles that are currently segregating in the US Holstein population. Results: The Pryce method and the modified Pryce method showed similar ability to reduce frequencies of recessive alleles, particularly for loci with frequencies greater than 0.30. The modified Pryce method outperformed the Pryce method for low-frequency alleles with small economic value. Cumulative genetic gain for the selection objective was slightly greater when using the Pryce method, but rates of inbreeding were similar across methods. Conclusions: The proposed method reduces allele frequencies faster than other methods, and also can be used to maintain or increase the frequency of desirable recessives. It can be easily implemented in software for mate allocation, and the code used in this study is freely available as a reference implementation. C1 [Cole, John B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Cole, JB (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM john.cole@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Agricultural Research Service of the United States Department of Agriculture [1265-31000-096-00]; Agriculture and Food Research Initiative Competitive [2013-68004-20365] FX The author would like to thank Sophie Eaglen and Paul VanRaden for several suggestions about the study, Kristen Parker Gaddis for computational and editorial assistance, and Daniel Null for technical editing of the manuscript. The editors and two anonymous reviewers provided important advice for improving the quality of the paper. This research was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information", of the Agricultural Research Service of the United States Department of Agriculture, and Agriculture and Food Research Initiative Competitive Grant No. 2013-68004-20365, "Improving Fertility of Dairy Cattle Using Translational Genomics". Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 41 TC 6 Z9 6 U1 2 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD NOV 30 PY 2015 VL 47 AR 94 DI 10.1186/s12711-015-0174-9 PG 13 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CX7DZ UT WOS:000365863100001 PM 26620491 ER PT J AU Magombedze, G Eda, S Stabel, J AF Magombedze, Gesham Eda, Shigetoshi Stabel, Judy TI Predicting the Role of IL-10 in the Regulation of the Adaptive Immune Responses in Mycobacterium avium Subsp paratuberculosis Infections Using Mathematical Models SO PLOS ONE LA English DT Article ID CD4(+) T-CELLS; CYTOKINE GENE-EXPRESSION; BLOOD MONONUCLEAR-CELLS; BOVINE PARATUBERCULOSIS; INTERFERON-GAMMA; IFN-GAMMA; PERIPHERAL-BLOOD; DENDRITIC CELLS; JOHNES-DISEASE; LONG-TERM AB Mycobacterium avium subsp. paratuberculosis (MAP) is an intracellular bacterial pathogen that causes Johne's disease (JD) in cattle and other animals. The hallmark of MAP infection in the early stages is a strong protective cell-mediated immune response (Th1-type), characterized by antigen-specific gamma-interferon (IFN-gamma). The Th1 response wanes with disease progression and is supplanted by a non-protective humoral immune response (Th2-type). Interleukin-10 (IL-10) is believed to play a critical role in the regulation of host immune responses to MAP infection and potentially orchestrate the reversal of Th1/Th2 immune dominance during disease progression. However, how its role correlates with MAP infection remains to be completely deciphered. We developed mathematical models to explain probable mechanisms for IL-10 involvement in MAP infection. We tested our models with IL-4, IL-10, IFN-gamma, and MAP fecal shedding data collected from calves that were experimentally infected and followed over a period of 360 days in the study of Stabel and Robbe-Austerman (2011). Our models predicted that IL-10 can have different roles during MAP infection, (i) it can suppress the Th1 expression, (ii) can enhance Th2 (IL-4) expression, and (iii) can suppress the Th1 expression in synergy with IL-4. In these predicted roles, suppression of Th1 responses was correlated with increased number of MAP. We also predicted that Th1-mediated responses (IFN-gamma) can lead to high expression of IL-10 and that infection burden regulates Th2 suppression by the Th1 response. Our models highlight areas where more experimental data is required to refine our model assumptions, and further test and investigate the role of IL-10 in MAP infection. C1 [Magombedze, Gesham] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. [Magombedze, Gesham] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, MRC Ctr Outbreak Anal & Modelling, London, England. [Eda, Shigetoshi] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Stabel, Judy] USDA ARS, Natl Anim Dis, Ames, IA 50010 USA. RP Magombedze, G (reprint author), Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. EM gmagombedze@nimbios.org OI Magombedze, Gesham/0000-0003-2745-3475 FU National Institute for Mathematical and Biological Synthesis (NIMBioS) - National Science Foundation [EF-0832858]; University of Tennessee, Knoxville FX The authors acknowledge the support of the National Institute for Mathematical and Biological Synthesis (NIMBioS), an Institute sponsored by the National Science Foundation through NSF Award #EF-0832858, with additional support from The University of Tennessee, Knoxville. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 4 Z9 4 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 30 PY 2015 VL 10 IS 11 AR e0141539 DI 10.1371/journal.pone.0141539 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7NW UT WOS:000365889800006 PM 26619346 ER PT J AU Gesch, RW Isbell, TA Oblath, EA Allen, BL Archer, DW Brown, J Hatfield, JL Jabro, JD Kiniry, JR Long, DS Vigil, MF AF Gesch, R. W. Isbell, T. A. Oblath, E. A. Allen, B. L. Archer, D. W. Brown, J. Hatfield, J. L. Jabro, J. D. Kiniry, J. R. Long, D. S. Vigil, M. F. TI Comparison of several Brassica species in the north central US for potential jet fuel feedstock SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article; Proceedings Paper CT AAIC International Conference on Advances in Industrial Crops and Products Worldwide CY SEP, 2014 CL Athens, GREECE DE Brassicaceae; Hydrotreated renewable jet fuel; Oilseeds; Seed yield; Oil yield ID APPLIED NITROGEN; SOWING DATE; SEED YIELD; CORN-BELT; CAMELINA; CARINATA; TILLAGE; GROWTH; SULFUR; WINTER AB Hydrotreated renewable jet fuel (HRJ) derived from crop oils has been commercially demonstrated, but full-scale production has been hindered by feedstock costs that make it more costly than petroleum-based fuels. Maintaining low feedstock costs while developing crops attractive to growers will be key to producing affordable HRJ and creating a dependable supply. Several Brassica oilseed species could potentially serve as feedstock, but genotypes agronomically and economically well suited for a given region will likely vary with environment and current cropping systems. The objectives of this study were to evaluate seed and seed oil yields of 12 summer annual Brassica genotypes representing six different species [Brassica napus L., Brassica rapa L, Brassica juncea L., Brassica carinata L., Sinapis alba L, and Camelina sativa (L.) Crantz.] and identify environmental factors that might limit their growth and oil production. The study was conducted during 2013 and 2014 in west central Minnesota, U.S. on a Barnes loam soil. This study is part of a larger project focused on evaluating the same set of oilseeds across the major wheat (Triticum aestivum L) growing areas of the U.S. Seed yields for the 12 springsown crops in Minnesota ranged from 1058 to 3718 kg ha(-1) in 2013 and 515 to 2020 kg ha(-1) in 2014. The range in seed oil yield was 287-1588 kg ha(-1) in 2013 and 210-885 kg ha(-1) in 2014. Plant lodging was a serious issue in 2013, but it varied widely among genotypes. In 2014, which was characterized by an abnormally wet spring, disease infection {most likely white leaf spot [Pseudocercosporella capsellae (Ellis & Everh.) Deighton 1973]} and flea beetle [Phyllotreta cruciferae (Goeze)] feeding led to plant damage, but these were primarily confined to certain Brassica napus cultivars. In west central Minnesota, certain B. napus canola lines and B. carinata produced the greatest seed and oil yields. B. carinata was the latest maturing species in the study, produced the highest biomass, and tended to have low harvest indices, indicating ample room for yield improvement. For any given ecoregion, striking a balance among crop yield, agricultural input costs, and optimum species/cultivar choice for a particular cropping system will be important for providing a reliable and affordable feedstock for HRJ. Published by Elsevier B.V. C1 [Gesch, R. W.] USDA ARS NCSCRL, Morris, MN 56267 USA. [Isbell, T. A.; Oblath, E. A.] USDA ARS NCAUR, Peoria, IL USA. [Allen, B. L.; Jabro, J. D.] USDA ARS NPARL, Sidney, MT 59270 USA. [Archer, D. W.] USDA ARS NGPRL, Mandan, ND 58554 USA. [Brown, J.] Univ Idaho, Moscow, ID 83844 USA. [Hatfield, J. L.] USDA ARS NLAE, Ames, IA 50011 USA. [Kiniry, J. R.] USDA ARS GSWRL, Temple, TX 76502 USA. [Long, D. S.] USDA ARS CPCRC, Pendleton, OR 97801 USA. [Vigil, M. F.] USDA ARS CGPRL, Akron, CO 80720 USA. RP Gesch, RW (reprint author), USDA ARS NCSCRL, Morris, MN 56267 USA. EM russ.gesch@ars.usda.gov; terry.isbell@ars.usda.gov; emily.oblath@ars.usda.gov; brett.allen@ars.usda.gov; david.archer@ars.udsa.gov; jbrown@uidaho.edu; jerry.hatfield@ars.usda.gov; jay.jabro@ars.usda.gov; jim.kiniry@ars.usda.gov; dan.long@ars.usda.gov; merle.vigil@ars.usda.gov FU USDA-National Institute of Food and Agriculture Biomass Research and Development Initiative [2012-10008-19727] FX The authors would like to thank Joe Boots, Chuck Hennen, and Scott Larsen for their expert field assistance. This work was supported through the USDA-National Institute of Food and Agriculture Biomass Research and Development Initiative award 2012-10008-19727. NR 25 TC 1 Z9 1 U1 5 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 30 PY 2015 VL 75 SI SI BP 2 EP 7 DI 10.1016/j.indcrop.2015.05.084 PN B PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CV4OP UT WOS:000364246700002 ER PT J AU Eberle, CA Thom, MD Nemec, KT Forcella, F Lundgren, JG Gesch, RW Riedell, WE Papiernik, SK Wagner, A Peterson, DH Eklund, JJ AF Eberle, Carrie A. Thom, Matthew D. Nemec, Kristine T. Forcella, Frank Lundgren, Jonathan G. Gesch, Russell W. Riedell, Walter E. Papiernik, Sharon K. Wagner, Angela Peterson, Dean H. Eklund, James J. TI Using pennycress, camelina, and canola cash cover crops to provision pollinators SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article; Proceedings Paper CT AAIC International Conference on Advances in Industrial Crops and Products Worldwide CY SEP, 2014 CL Athens, GREECE DE Thlaspi arvense; Camelina sativa; Brassica napus L.; Pollinators; Cover crops; Oil seeds ID THLASPI-ARVENSE L.; NORTHERN CORN-BELT; WILD BEE ABUNDANCE; HONEY-BEES; COLONY DEVELOPMENT; POLLEN NUTRITION; UNITED-STATES; BIODIESEL; OIL; DECLINE AB As pollinator decline continues, the-need to provide high value forage for insects increases. Finding agricultural crops to diversify the landscape and provide forage is one way to improve pollinator health. Three winter industrial oilseed crops (pennycress, winter camelina, and winter canola) were grown in Morris, Minnesota, and Brookings, South Dakota, during the winters of 2012-2013 and 2013-2014. Each of the three crops has pollinator-friendly flowers and value as winter cover crops and cash seed crops. The crops were evaluated for pollinator use, nectar production, green cover, and yield. Pennycress, camelina, and canola flowers had high insect activity with maximum visitation rates of 67 +/- 11.5, 22 +/- 3.1, and 61 +/- 6.8 insects min(-1). Cumulative nectar produced by pennycress, camelina, and canola was 13, 100, and 82 kg of sugar ha(-1) during the 2014 anthesis period, providing an important food resource to pollinators during early spring when there is little else on the agricultural landscape-that is blooming. Green cover in early spring ranged from 0 to 60% amongst the three crops, with camelina providing >25% green cover across all four site-years. Maximum seed yields were 1.1 +/- 0.04, 1.41 +/- 0.05, and 1.2 +/- 0.19 Mg ha(-1) for pennycress, camelina, and canola, respectively, which are economically viable harvests. Of the three crops, winter camelina provided the highest combined agroecosystem value through pollinator resources, green cover, and seed yields. (C) 2015 Elsevier B.V. All rights reserved. C1 [Eberle, Carrie A.; Thom, Matthew D.; Forcella, Frank; Gesch, Russell W.; Peterson, Dean H.; Eklund, James J.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Nemec, Kristine T.; Lundgren, Jonathan G.; Riedell, Walter E.; Papiernik, Sharon K.; Wagner, Angela] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Eberle, CA (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM carrie.eberle@ars.usda.gov; matt.thom@ars.usda.gov; kristine.nemec@ars.usda.gov; frank.forcella@ars.usda.gov; jonathan.lundgren@ars.usda.gov; russ.gesch@ars.usda.gov; walter.riedell@ars.usda.gov; sharon.papiernik@ars.usda.gov; angela.wagner@ars.usda.gov; dean.peterson@ars.usda.gov; jim.eklund@ars.usda.gov FU USDA-National Institute of Food and Agriculture [2012-67009-20272]; USDA-ARS FX We would like to thank Dave Schneider and Chris Nelson for their work in managing the SD field site. We thank Janet Fergen, Angela Wagner, Ryan Bell, Nicole Berg, Marissa Layman, Jacob Pecenka, and Cally Strobel for assistance with insect surveys. We would like to thank Chuck Hennen and Scott Larson for their input and management of the MN field sites. This work was supported through the USDA-National Institute of Food and Agriculture award 2012-67009-20272, as well as a USDA-ARS post-doctoral research award. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 4 Z9 4 U1 4 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 30 PY 2015 VL 75 SI SI BP 20 EP 25 DI 10.1016/j.indcrop.2015.06.026 PN B PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CV4OP UT WOS:000364246700005 ER PT J AU Berti, M Gesch, R Johnson, B Ji, Y Seames, W Aponte, A AF Berti, Marisol Gesch, Russ Johnson, Burton Ji, Yun Seames, Wayne Aponte, Alfredo TI Double- and relay-cropping of energy crops in the northern Great Plains, USA SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article; Proceedings Paper CT AAIC International Conference on Advances in Industrial Crops and Products Worldwide CY SEP, 2014 CL Athens, GREECE DE Sorghum; Camelina; Seed yield; Biomass; Bioenergy; Energy efficiency ID ECOSYSTEM SERVICES; CORN-BELT; WINTER CAMELINA; SORGHUM; YIELD; SYSTEMS; GROWTH; WHEAT; MONOCULTURES; BIOFUEL AB In a growing developing world, innovative cropping systems are necessary to obtain continuous and sustainable supplies of food, feed, fuel, and bio-based products. Double- and relay-cropping systems are an option to produce biofuels, food, and biomass feedstock in a single season on the same land without sacrificing food security. Field studies were conducted between 2011 and 2013 in Prosper and Carrington, ND, and Morris, MN. Eleven crop sequences composed of double- and relay-cropping of forage sorghum (Sorghum bicolor L.), soybean (Glycine max (L) Merr.), and maize (Zea mays L), following winter camelina (Camelina sativa L) were evaluated and compared with soybean, maize, and sorghum monocultures. Forage sorghum and camelina were used as theoretical feedstocks for energy production from biomass and oil, respectively. Camelina seed yield was 1415 and 940 kg ha(-1) in Prosper and Carrington, respectively, when averaged across years. In Morris, camelina seed yield averaged 278, and 1745 kg ha(-1) in 2012 and 2013, respectively. In the relay-cropping systems, the highest biomass yield (16.2 Mg ha(-1)) was achieved with forage sorghum inter-seeded into standing camelina. As expected, all sorghum, soybean, and maize monocultures had higher biomass yield than the same crops in double- or relay-cropping with camelina. Energy efficiency was evaluated for double- and relay-cropping systems based on energy inputs and outputs. The forage sorghum seeded at a normal seeding date (NSD) and at the same time that it was seeded into the double-crop treatments (DSD) on fallow ground, and soybean seeded at a NSD had energy efficiencies of 17.8, 18.7, and 21.6, respectively, in Carrington. In Morris, forage sorghum and maize seeded at NSD had energy efficiencies of 42.6 and 34.7, respectively. Of the double- and relay-cropping systems, the camelina-forage sorghum relay treatment produced the highest energy efficiency at all three locations in both years. Forage sorghum seeded at NSD was the most energy efficient monocrop at all three locations. Both forage sorghum in monocrop and the camelina-sorghum relay treatment showed good potential for biofuel and energy feedstock production in the northern Great Plains. (C) 2015 Elsevier B.V. All rights reserved. C1 [Berti, Marisol; Johnson, Burton; Aponte, Alfredo] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Gesch, Russ] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Ji, Yun; Seames, Wayne] Univ N Dakota, Dept Chem Engn, Grand Forks, ND 58202 USA. RP Berti, M (reprint author), N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. EM Marisol.berti@ndsu.edu FU North Central Sun Grant Initiative [3TG114] FX This research was funded in part by the North Central Sun Grant Initiative, award number: 3TG114. Authors also wish to thank all technical staff, undergraduate, and graduate students that helped support these studies. NR 44 TC 2 Z9 2 U1 8 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 30 PY 2015 VL 75 SI SI BP 26 EP 34 DI 10.1016/j.indcrop.2015.05.012 PN B PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA CV4OP UT WOS:000364246700006 ER PT J AU Boggs, J Sun, G Domec, JC McNulty, S Treasure, E AF Boggs, Johnny Sun, Ge Domec, Jean-Christophe McNulty, Steven Treasure, Emrys TI Clearcutting upland forest alters transpiration of residual trees in the riparian buffer zone SO HYDROLOGICAL PROCESSES LA English DT Article; Proceedings Paper CT 3rd International Conference on Forests and Water in a Changing Environment CY 2012 CL Fukuoka, JAPAN DE sap flux density; riparian buffer; transpiration; stomatal conductance; Best Management Practice (BMP); North Carolina ID VAPOR-PRESSURE DEFICIT; XYLEM SAP FLOW; STOMATAL CONDUCTANCE; WATER FLUX; CANOPY TRANSPIRATION; DECIDUOUS FOREST; SAPWOOD AREA; PINUS-TAEDA; LEAF-AREA; STAND AB Our objectives are (1) to compare tree sap flux density (J(s) in g cm(-2) d(-1)) and stomatal conductance (G(s) in mmol m(-2) s(-1)) across five dominant species, red maple (Acer rubrum), sweetgum (Liquidambar styraciflua), tulip poplar (Liriodendron tulipifera), loblolly pine (Pinus taeda), and oak species (Quercus spp.), (2) to quantity riparian buffer stand transpiration (E-s in mm d(-1)), and (3) to link riparian buffer E-s of residual trees to stream discharge. In June 2010, the above species were instrumented with sap flow sensors in a pair (HF1 and HF2) of 12 hectare gauged watersheds. HF1 was clearcut, leaving a 15.2-m riparian buffer around the stream, and HF2 was the reference. Trees were harvested in the riparian buffer reducing HF1 riparian buffer basal area by 27%. The riparian buffer growing season net radiation increased from 11.9 W m(-2) preharvest to an average of 24.3 W m(-2) postharvest. HF1 stream growing season discharge increased dramatically (150%) from the preharvest to postharvest period. HF1 2010 preharvest growing season soil moisture was 22.5%. HF1 postharvest growing season soil moisture was 28.5% in 2011, 26.5% in 2012, and 27.2% in 2013. HF2 canopy cover, energy input, and soil moisture showed little change over the same period. From preharvest to postharvest, mean daily growing season J(s) of trees in HF1 increased in all species. A reduction in HF1 Gs was less evident over the study vapour pressure deficit range in loblolly pine, red maple, and tulip poplar than in oak and sweetgum during the postharvest period. HF1 residual trees in the riparian buffer used 43% more water in growing season postharvest (314 mm) than growing preharvest (220 mm) period. This resulted in an 8% reduction in stream discharge because of an increase in riparian buffer E-s. Although clearcutting increased stream discharge, we conclude that the increase in transpiration by the residual trees in the riparian buffer will, at least, partially mitigate the hydrologic effects of forest removal through increased transpiration. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Boggs, Johnny; Sun, Ge; McNulty, Steven; Treasure, Emrys] US Forest Serv, USDA, Raleigh, NC 27695 USA. [Domec, Jean-Christophe] N Carolina State Univ, Raleigh, NC 27695 USA. RP McNulty, S (reprint author), US Forest Serv, USDA, Raleigh, NC 27695 USA. EM smcnulty@fs.fed.us FU US EPA Non-Point Source (NPS) Pollution Control Grant through Section 319(h) of the Clean Water Act, NC Forest Service; US Forest Service Eastern Forest Environmental Threat Assessment Center FX This research was funded by the US EPA Non-Point Source (NPS) Pollution Control Grant through Section 319(h) of the Clean Water Act, NC Forest Service, and US Forest Service Eastern Forest Environmental Threat Assessment Center. We would like to thank the many students and support staff for their diligent work in the field and laboratory over the years. We would also like to especially thank William 'Bill' Swartley, Tom Gerow, and David Jones from the NC Forest Service for their project support and guidance on the Timber Harvest portion of this study. NR 60 TC 3 Z9 3 U1 4 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD NOV 29 PY 2015 VL 29 IS 24 SI SI BP 4979 EP 4992 DI 10.1002/hyp.10474 PG 14 WC Water Resources SC Water Resources GA DB1OC UT WOS:000368276600002 ER PT J AU Sun, G Caldwell, PV McNulty, SG AF Sun, Ge Caldwell, Peter V. McNulty, Steven G. TI Modelling the potential role of forest thinning in maintaining water supplies under a changing climate across the conterminous United States SO HYDROLOGICAL PROCESSES LA English DT Article; Proceedings Paper CT 3rd International Conference on Forests and Water in a Changing Environment CY 2012 CL Fukuoka, JAPAN DE forest thinning; climate change; water yield ID COASTAL-PLAIN; STREAMFLOW; CARBON; VARIABILITY; VEGETATION; IMPACTS; TREES; PINE; US; PRECIPITATION AB The goal of this study was to test the sensitivity of water yield to forest thinning and other forest management/disturbances and climate across the conterminous United States (CONUS). Leaf area index (LAI) was selected as a key parameter linking changes in forest ecosystem structure and functions. We used the Water Supply Stress Index model to examine water yield response under 18 scenarios that combine hypothetical LAI changes (+10%, +/- 20%, -50%, and -80%), uniform increases in temperature (+1 degrees C and +2 degrees C) and precipitation change (+/- 10%), and four climate change scenarios projected by general circulation models (GCMs) for the year 2050. Approximately 2100 large basins produced approximately 2003 billion cubic metres of water annually from 2002 to 2007. Forest lands covered 23% of the land surface area, but contributed 43% of the total water yield for the CONUS. As a whole, water yield increased by 3%, 8%, and 13% when LAI was reduced 20%, 50%, and 80%, respectively, while water yield decreased by 3% when LAI increased by 20%. Temperature increases of 2 degrees C alone could decrease water yield by 11%. A reduction of precipitation by 10% and 20% could result in a decrease of water yield by 20% and 39%, respectively. The direction and magnitude of water yield response to the combinations of LAI (+10%), climate warming (+1 degrees C), and precipitation change (+/- 10%) were dominated by the change in precipitation. Climate change projected by the four GCMs (CSIROMK2 B2, CSIROMK3.5 A1B, HADCM3 B2, and MIROC32 A1B) resulted in a large change in water yield (+18% to -64%) by 2045-2055 when compared with the baseline. A 50% reduction in forest LAI under the four GCMs scenarios could greatly mitigate or exacerbate future climate change impacts on water yield in forest-dominated watersheds with high precipitation. This study provides the first quantitative estimate of the effects of forest thinning options on water yield under future climate across the CONUS. Effective forest water management for climate mitigation should focus on those watersheds identified. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Sun, Ge; McNulty, Steven G.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Caldwell, Peter V.] US Forest Serv, Coweeta Hydrol Lab, Southern Res Stn, USDA, Otto, NC 27606 USA. RP Sun, G (reprint author), US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. EM gesun@fs.fed.us FU Eastern Forest Environmental Threat Assessment Center, Southern Research Station; USDA Forest Service FX The authors thank two anonymous reviewers for their insight that helped improve the early version of this paper. Funding is provided by the Eastern Forest Environmental Threat Assessment Center, Southern Research Station, and USDA Forest Service. Erika Mack and Jennifer Moore Myers provided technical support. NR 71 TC 5 Z9 5 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD NOV 29 PY 2015 VL 29 IS 24 SI SI BP 5016 EP 5030 DI 10.1002/hyp.10469 PG 15 WC Water Resources SC Water Resources GA DB1OC UT WOS:000368276600005 ER PT J AU Il Seo, J Nakamura, F Chun, KW Kim, SW Grant, GE AF Il Seo, Jung Nakamura, Futoshi Chun, Kun Woo Kim, Suk Woo Grant, Gordon E. TI Precipitation patterns control the distribution and export of large wood at the catchment scale SO HYDROLOGICAL PROCESSES LA English DT Article; Proceedings Paper CT 3rd International Conference on Forests and Water in a Changing Environment CY 2012 CL Fukuoka, JAPAN DE large wood distribution; disturbance regime; channel morphology; supply-limited; transport-limited; Japanese archipelago ID MOUNTAIN STREAMS; CHANNEL MORPHOLOGY; FLUVIAL PROCESSES; WESTERN CASCADES; DEBRIS; RIVER; DYNAMICS; OREGON; STORAGE; USA AB Large wood (LW) plays an important role in river ecosystems, but LW-laden floods may cause serious damage to human lives and property. The relationship between precipitation patterns and variations in LW distribution and export at the watershed scale is poorly understood. To explore these linkages, we examined differences in LW distribution as a function of channel morphologies in six watersheds located in southern and northern Japan and analysed the impacts of different precipitation patterns on the fluvial export of LW from river catchments. In southern Japan, intense rainfalls caused by typhoons or localized torrential downpours initiate landslides and debris flows that introduce massive amounts of LW into channels. Gravel bars formed by frequent flood events are widely prevalent, and the LW temporarily stored on these bars is frequently moved and/or broken into smaller pieces by floods. In these systems fluvial export of LW is supply-limited, with smaller accumulations and shorter residence times than in northern Japan. Conversely, in northern Japan, where typhoons and torrential downpours rarely occur, LW is mostly recruited by bank erosion, tree mortality and windthrow into channels, rather than by landslides and debris flows. Recruited pieces accumulate in log jams on valley floors, particularly on floodplains supporting mature forests, resulting in larger accumulations and longer residence times. In these watersheds fluvial export of LW is transport-limited, and the pieces gradually decompose during long-term storage as log jams. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Il Seo, Jung] Kongju Natl Univ, Coll Ind Sci, Dept Forest Resources, Yesan 340702, Chungcheongnamd, South Korea. [Nakamura, Futoshi] Hokkaido Univ, Grad Sch Agr, Dept Forest Sci, Kita Ku, Sapporo, Hokkaido 0608589, Japan. [Chun, Kun Woo; Kim, Suk Woo] Kangwon Natl Univ, Dept Forest Resources, Coll Forest & Environm Sci, Chunchon 200701, Gangwondo, South Korea. [Grant, Gordon E.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Nakamura, F (reprint author), Hokkaido Univ, Grad Sch Agr, Dept Forest Sci, Kita Ku, Kita 9 Nishi 9, Sapporo, Hokkaido 0608589, Japan. EM nakaf@for.agr.hokudai.ac.jp OI Nakamura, Futoshi/0000-0003-4351-2578 FU Water Resources Environment Technology Center; Ministry of Education, Science Culture, Japan [23248021]; Korea Forest Service, Republic of Korea [S111214L050110] FX The authors thank the Water Resources Environment Technology Center and the Japanese Ministry of Land, Infrastructure and Transport for assistance in providing and collecting data on large wood. The authors also thank Yong Suk Kim and Takumi Akasaka for valuable advice in the field. This research was funded by the Water Resources Environment Technology Center and the Grants in Aid for Scientific Research (No. 23248021) from the Ministry of Education, Science & Culture, Japan. In addition, this research was conducted in part with the support of Forest Science & Technology Projects (No. S111214L050110) provided by the Korea Forest Service, Republic of Korea. NR 47 TC 0 Z9 0 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD NOV 29 PY 2015 VL 29 IS 24 SI SI BP 5044 EP 5057 DI 10.1002/hyp.10473 PG 14 WC Water Resources SC Water Resources GA DB1OC UT WOS:000368276600007 ER PT J AU Shukitt-Hale, B Bielinski, DF Lau, FC Willis, LM Carey, AN Joseph, JA AF Shukitt-Hale, Barbara Bielinski, Donna F. Lau, Francis C. Willis, Lauren M. Carey, Amanda N. Joseph, James A. TI The beneficial effects of berries on cognition, motor behaviour and neuronal function in ageing SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Strawberries; Blueberries; Spatial memory; Learning; Stress signalling; Neurogenesis ID OXIDATIVE STRESS; BLUEBERRY SUPPLEMENTATION; DIETARY SUPPLEMENTATION; SIGNAL-TRANSDUCTION; ALZHEIMERS-DISEASE; RADICAL HYPOTHESIS; INDUCED DECREMENTS; FRUIT EXTRACTS; OLDER-ADULTS; COS-7 CELLS AB Previously, it has been shown that strawberry (SB) or blueberry (BB) supplementations, when fed to rats from 19 to 21 months of age, reverse age-related decrements in motor and cognitive performance. We have postulated that these effects may be the result of a number of positive benefits of the berry polyphenols, including decreased stress signalling, increased neurogenesis, and increased signals involved in learning and memory. Thus, the present study was carried out to examine these mechanisms in aged animals by administering a control, 2 % SB-or 2 % BB-supplemented diet to aged Fischer 344 rats for 8 weeks to ascertain their effectiveness in reversing age-related deficits in behavioural and neuronal function. The results showed that rats consuming the berry diets exhibited enhanced motor performance and improved cognition, specifically working memory. In addition, the rats supplemented with BB and SB diets showed increased hippocampal neurogenesis and expression of insulin-like growth factor 1, although the improvements in working memory performance could not solely be explained by these increases. The diverse polyphenolics in these berry fruits may have additional mechanisms of action that could account for their relative differences in efficacy. C1 [Shukitt-Hale, Barbara; Bielinski, Donna F.; Lau, Francis C.; Willis, Lauren M.; Carey, Amanda N.; Joseph, James A.] Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov FU USDA intramural funds; USDA; Wild Blueberry Association of North America (WBANA); US Highbush Blueberry Council (USHBC); California Strawberry Commission FX This research was supported by USDA intramural funds and agreements between the USDA and the Wild Blueberry Association of North America (WBANA), the US Highbush Blueberry Council (USHBC) and the California Strawberry Commission. NR 52 TC 6 Z9 6 U1 12 U2 37 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD NOV 28 PY 2015 VL 114 IS 10 BP 1542 EP 1549 DI 10.1017/S0007114515003451 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CW4JP UT WOS:000364957900002 PM 26392037 ER PT J AU Xue, SS Uppugundla, N Bowman, MJ Cavalier, D Sousa, LDC Dale, BE Balan, V AF Xue, Saisi Uppugundla, Nirmal Bowman, Michael J. Cavalier, David Sousa, Leonardo Da Costa Dale, Bruce. E. Balan, Venkatesh TI Sugar loss and enzyme inhibition due to oligosaccharide accumulation during high solids-loading enzymatic hydrolysis SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Recalcitrant oligosaccharides; High solids-loading; Commercial enzymes; AFEX-CS hydrolysate; Charcoal fractionation; Size exclusion chromatography; Enzyme inhibition ID TRICHODERMA-REESEI CELLULASES; SWITCHGRASS PANICUM-VIRGATUM; FIBER EXPANSION AFEX; LIGNOCELLULOSIC BIOMASS; DILUTE-ACID; CORN STOVER; CELLOBIOHYDROLASE I; ECONOMIC-EVALUATION; IONIC LIQUID; L. XYLAN AB Background: Accumulation of recalcitrant oligosaccharides during high-solids loading enzymatic hydrolysis of cellulosic biomass reduces biofuel yields and increases processing costs for a cellulosic biorefinery. Recalcitrant oligosaccharides in AFEX-pretreated corn stover hydrolysate accumulate to the extent of about 18-25 % of the total soluble sugars in the hydrolysate and 12-18 % of the total polysaccharides in the inlet biomass (untreated), equivalent to a yield loss of about 7-9 kg of monomeric sugars per 100 kg of inlet dry biomass (untreated). These oligosaccharides represent a yield loss and also inhibit commercial hydrolytic enzymes, with both being serious bottlenecks for economical biofuel production from cellulosic biomass. Very little is understood about the nature of these oligomers and why they are recalcitrant to commercial enzymes. This work presents a robust method for separating recalcitrant oligosaccharides from high solid loading hydrolysate in gramme quantities. Composition analysis, recalcitrance study and enzyme inhibition study were performed to understand their chemical nature. Results: Oligosaccharide accumulation occurs during high solid loading enzymatic hydrolysis of corn stover (CS) irrespective of using different pretreated corn stover (dilute acid: DA, ionic liquids: IL, and ammonia fibre expansion: AFEX). The methodology for large-scale separation of recalcitrant oligosaccharides from 25 % solids-loading AFEX-corn stover hydrolysate using charcoal fractionation and size exclusion chromatography is reported for the first time. Oligosaccharides with higher degree of polymerization (DP) were recalcitrant towards commercial enzyme mixtures [Ctec2, Htec2 and Multifect pectinase (MP)] compared to lower DP oligosaccharides. Enzyme inhibition studies using processed substrates (Avicel and xylan) showed that low DP oligosaccharides also inhibit commercial enzymes. Addition of monomeric sugars to oligosaccharides increases the inhibitory effects of oligosaccharides on commercial enzymes. Conclusion: The carbohydrate composition of the recalcitrant oligosaccharides, ratios of different DP oligomers and their distribution profiles were determined. Recalcitrance and enzyme inhibition studies help determine whether the commercial enzyme mixtures lack the enzyme activities required to completely de-polymerize the plant cell wall. Such studies clarify the reasons for oligosaccharide accumulation and contribute to strategies by which oligosaccharides can be converted into fermentable sugars and provide higher biofuel yields with less enzyme. C1 [Xue, Saisi; Uppugundla, Nirmal; Cavalier, David; Sousa, Leonardo Da Costa; Dale, Bruce. E.; Balan, Venkatesh] Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, Biomass Conver Res Lab BCRL, Chem Engn & Mat Sci, Lansing, MI 48910 USA. [Bowman, Michael J.] ARS, USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. [Cavalier, David] Michigan State Univ, DOE Plant Res Lab, E Lansing, MI 48824 USA. RP Xue, SS (reprint author), Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, Biomass Conver Res Lab BCRL, Chem Engn & Mat Sci, 3815 Technol Blvd, Lansing, MI 48910 USA. EM xuesaisi@egr.msu.edu; nirmal@egr.msu.edu; balan@egr.msu.edu OI Xue, Saisi/0000-0002-1973-0666 FU DOE Great Lakes Bioenergy Research Center - US. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-FC02-07ER64494] FX This work was funded by the DOE Great Lakes Bioenergy Research Center (http://www.greatlakesbioenergy.org) supported by the US. Department of Energy, Office of Science, Office of Biological and Environmental Research, through Cooperative Agreement DE-FC02-07ER64494 between The Board of Regents of the University of Wisconsin System and the US. Department of Energy. Special thanks to Novozyme Inc. and DuPont Industrial Biosciences for the generous gift of enzymes. We also thank Lee Alexander for contributing to production of ACSH and oligosaccharides, Pete Donald who helped carry out the AFEX pretreatment, and special thanks to Christa Gunawan for performing HPLC analysis for sugar composition and significant suggestions on revising the manuscript. NR 56 TC 5 Z9 5 U1 13 U2 39 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD NOV 26 PY 2015 VL 8 AR 195 DI 10.1186/s13068-015-0378-9 PG 14 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA CX6AT UT WOS:000365784300002 PM 26617670 ER PT J AU Li, XL Lian, FZ Liu, C Hu, KQ Wang, XD AF Li, Xinli Lian, Fuzhi Liu, Chun Hu, Kang-Quan Wang, Xiang-Dong TI Isocaloric Pair-Fed High-Carbohydrate Diet Induced More Hepatic Steatosis and Inflammation than High-Fat Diet Mediated by miR-34a/SIRT1 Axis in Mice SO SCIENTIFIC REPORTS LA English DT Article ID MESSENGER-RNA LEVELS; Y GASTRIC BYPASS; LIVER-DISEASE; NONALCOHOLIC STEATOHEPATITIS; APO-10'-LYCOPENOIC ACID; OBESE RATS; X RECEPTOR; LYCOPENE METABOLITE; ENERGY-EXPENDITURE; SIRT1 ACTIVITY AB To investigate the different effects of isocaloric high-fat diet (HFD) and high-carbohydrate diet (HCD) on hepatic steatosis and the underlying mechanisms, especially the role of microRNA-34a/silent information regulator T1 (SIRT1) axis, C57BL/6J mice (n = 12/group) were isocaloric pair-fed with Lieber-DeCarli liquid diet containing either high fat (HFLD) or high carbohydrate (HCLD) for 16 weeks. As compared to the HFLD fed mice, despite the similar final body weights, HCLD feeding: (1) induced more severe hepatic steatosis; (2) up-regulated hepatic expression of miR-34a accompanied with significant decrease of SIRT1 and nicotinamide phosphoribosyltransferase (NAMPT), SIRT1 activity and phosphorylation of AMPK; (3) up-regulated de novo lipogenesis (DNL) related proteins expression (ACC, SCD1), and down-regulated expressions of miR-122, miR-370 and miR-33; (4) decreased mRNA expressions of genes Cpt1, Ppara and Pgc1a related to fatty acid oxidation; (5) increased hepatic total cholesterol concentration and decreased expression of cholesterol metabolism related genes Abcg5, Abcg8, Abcg11, Cyp7a1 and Cyp8b1; and (6) induced higher hepatic inflammatory response accompanied with significant increased mRNA expressions of Il1 beta, Tnf alpha and Mcp1. Thus, isocaloric HCLD feeding induced greater severity in hepatic steatosis and inflammatory response than HFLD feeding, potentially through miR-34a/SIRT1 axis mediated promotion of DNL, inhibition of fatty acid oxidation and cholesterol metabolism. C1 [Li, Xinli; Lian, Fuzhi; Liu, Chun; Hu, Kang-Quan; Wang, Xiang-Dong] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Boston, MA 02111 USA. [Li, Xinli] Soochow Univ, Coll Med, Sch Publ Hlth, Suzhou 215123, Jiangsu, Peoples R China. RP Wang, XD (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu RI Lian, Fuzhi/F-9083-2015 OI Lian, Fuzhi/0000-0002-0733-579X FU State Scholarship Fund, China Scholarship Council [2011832272]; NIH [CA176256]; USDA/ARS [58-1950-074] FX The authors would like to thank John Lomartire for his assistance on this manuscript. Dr. Xinli Li was supported by a scholarship (File No. 2011832272) from the State Scholarship Fund by The China Scholarship Council. This paper was supported by the NIH grant (CA176256) and USDA/ARS grant (#58-1950-074). Any opinions, findings, conclusions, and recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the sponsors. NR 58 TC 0 Z9 0 U1 7 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 26 PY 2015 VL 5 AR 16774 DI 10.1038/srep16774 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX0KP UT WOS:000365385600001 PM 26608583 ER PT J AU Heslot, N Jannink, JL AF Heslot, Nicolas Jannink, Jean-Luc TI An alternative covariance estimator to investigate genetic heterogeneity in populations SO GENETICS SELECTION EVOLUTION LA English DT Article ID GENOME-WIDE ASSOCIATION; RELATIONSHIP MATRIX; RIDGE-REGRESSION; BREEDING VALUES; SELECTION; MODEL; PREDICTION; ACCURACY; CATTLE; MAIZE AB Background: For genomic prediction and genome-wide association studies (GWAS) using mixed models, covariance between individuals is estimated using molecular markers. Based on the properties of mixed models, using available molecular data for prediction is optimal if this covariance is known. Under this assumption, adding individuals to the analysis should never be detrimental. However, some empirical studies showed that increasing training population size decreased prediction accuracy. Recently, results from theoretical models indicated that even if marker density is high and the genetic architecture of traits is controlled by many loci with small additive effects, the covariance between individuals, which depends on relationships at causal loci, is not always well estimated by the whole-genome kinship. Results: We propose an alternative covariance estimator named K-kernel, to account for potential genetic heterogeneity between populations that is characterized by a lack of genetic correlation, and to limit the information flow between a priori unknown populations in a trait-specific manner. This is similar to a multi-trait model and parameters are estimated by REML and, in extreme cases, it can allow for an independent genetic architecture between populations. As such, K-kernel is useful to study the problem of the design of training populations. K-kernel was compared to other covariance estimators or kernels to examine its fit to the data, cross-validated accuracy and suitability for GWAS on several datasets. It provides a significantly better fit to the data than the genomic best linear unbiased prediction model and, in some cases it performs better than other kernels such as the Gaussian kernel, as shown by an empirical null distribution. In GWAS simulations, alternative kernels control type I errors as well as or better than the classical whole-genome kinship and increase statistical power. No or small gains were observed in cross-validated prediction accuracy. Conclusions: This alternative covariance estimator can be used to gain insight into trait-specific genetic heterogeneity by identifying relevant sub-populations that lack genetic correlation between them. Genetic correlation can be 0 between identified sub-populations by performing automatic selection of relevant sets of individuals to be included in the training population. It may also increase statistical power in GWAS. C1 [Heslot, Nicolas; Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Heslot, Nicolas] Limagrain Europe, F-63720 Chappes, France. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Heslot, N (reprint author), Cornell Univ, Dept Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. EM nh269@cornell.edu FU USDA-NIFA-AFRI grants [2009-65300-05661, 2011-68002-30029, 2005-05130]; Limagrain Europe FX This research was supported in part by USDA-NIFA-AFRI grants, award numbers 2009-65300-05661, 2011-68002-30029, and 2005-05130. Limagrain Europe provided financial support for N. Heslot. We thank the reviewers and the editor for their comments and suggestions which substantially improved the manuscript. NR 36 TC 2 Z9 2 U1 0 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD NOV 26 PY 2015 VL 47 AR 93 DI 10.1186/s12711-015-0171-z PG 11 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CW9SN UT WOS:000365338600002 PM 26612537 ER PT J AU Zhou, L Matsumoto, T Tan, HW Meinhardt, LW Mischke, S Wang, BY Zhang, DP AF Zhou, Lin Matsumoto, Tracie Tan, Hua-Wei Meinhardt, Lyndel W. Mischke, Sue Wang, Boyi Zhang, Dapeng TI Developing single nucleotide polymorphism markers for the identification of pineapple (Ananas comosus) germplasm SO HORTICULTURE RESEARCH LA English DT Article ID SSR MARKERS; DOMESTICATION; DIVERSITY; CULTIVARS; INFERENCE; PROGRAM AB Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using expressed sequence tag and nucleotide sequences from public databases, we developed 213 single nucleotide polymorphism (SNP) markers and validated 96 SNPs by genotyping the United States Department of Agriculture - Agricultural Research Service pineapple germplasm collection, maintained in Hilo, Hawaii. The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection. Twenty-four groups of duplicates were detected, encompassing 130 of the total 170 A cosmos accessions. The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple. Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties. Parentage analysis further revealed that both A. comosus var. bracteatus and A. comosus var. ananassoides are likely progenitors of pineapple cultivars. However, the traditional classification of cultivated pineapple into horticultural groups (e.g. 'Cayenne', 'Spanish', 'Queen') was not well supported by the present study. These SNP markers provide robust and universally comparable DNA fingerprints; thus, they can serve as an efficient genotyping tool to assist pineapple germplasm management, propagation of planting material, and pineapple cultivar protection. The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops. C1 [Zhou, Lin; Meinhardt, Lyndel W.; Mischke, Sue; Zhang, Dapeng] USDA ARS, Beltsville Agr Res Ctr, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Matsumoto, Tracie] USDA ARS, Daniel K Inouye Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Zhou, Lin; Tan, Hua-Wei] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Zhou, Lin; Tan, Hua-Wei] Yunnan Forestry Technol Coll, Kunming 650224, Yunnan, Peoples R China. RP Zhang, DP (reprint author), USDA ARS, Beltsville Agr Res Ctr, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. EM Dapeng.Zhang@ars.usda.gov NR 57 TC 1 Z9 1 U1 5 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD NOV 25 PY 2015 VL 2 AR UNSP 15056 DI 10.1038/hortres.2015.56 PG 12 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA CY1CB UT WOS:000366143200002 PM 26640697 ER PT J AU Watson, GW Williams, DJ Miller, DR AF Watson, Gillian W. Williams, Douglas J. Miller, Douglass R. TI The identity and distribution of Fiorinia phantasma (Cockerell & Robinson) (Hemiptera: Coccomorpha: Diaspididae), with a new synonym SO ZOOTAXA LA English DT Article DE morphology; synonymy; distribution; host range; natural enemies ID RECORDS; CHINA AB The morphologies of Fiorinia phantasma (Cockerell & Robinson) (Hemiptera: Coccomorpha: Diaspididae) and F. coronata Williams & Watson are reviewed, and the name F. coronata is placed as a junior synonym of the name F. phantasma syn. n. The known geographical distribution and host range of F. phantasma is documented and discussed. An identification key to 12 of the 16 species of Fiorinia known from the Australasian, Nearctic and Neotropical Regions is provided. C1 [Watson, Gillian W.] Calif Dept Food & Agr, Sacramento, CA 95832 USA. [Williams, Douglas J.] Nat Hist Museum, Dept Life Sci Entomol, London SW7 5BD, England. [Miller, Douglass R.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD USA. [Miller, Douglass R.] Dept Plant Ind, Gainesville, FL USA. RP Watson, GW (reprint author), Calif Dept Food & Agr, 3294 Meadowview Rd, Sacramento, CA 95832 USA. EM gillian.watson@cdfa.ca.gov; Douglass.Miller@ARS.USDA.GOV NR 23 TC 1 Z9 1 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD NOV 25 PY 2015 VL 4048 IS 2 BP 291 EP 300 PG 10 WC Zoology SC Zoology GA CX1IZ UT WOS:000365451200009 PM 26624751 ER PT J AU Buffington, ML Copeland, RS AF Buffington, M. L. Copeland, R. S. TI Muhaka icipe, an enigmatic new genus and species of Kleidotomini (Hymenoptera: Figitidae: Eucoilinae) from an East African coastal forest SO JOURNAL OF NATURAL HISTORY LA English DT Article DE Cynipoidea; head morphology; Afrotropical Region ID KENYA; CYNIPOIDEA; REVISION; TANZANIA; CONSERVATION; STENTORCEPS; PATTERNS AB A remarkable new eucoiline genus and species, Muhaka icipe, is described herein. The genus is clearly a Kleidotomini, but is distinguished from other genera in the tribe by a unique head and scutellar morphology. The genus belongs to the wedge-head'-syndrome group of species that, to date, is unique to Afrotropical eucoilines. The new genus and species is reminiscent of Stentorceps Quinlan and Nanocthulhu Buffington, but is readily distinguished from these genera. Muhaka was collected from a threatened kaya (sacred forest) of coastal Kenya. The biological importance of this and other kaya forests, as well as their protection, is discussed.http://www.zoobank.org/urn:lsid:zoobank.org:pub:6918ED2C-69A4-48FC-A1E4-2B5DFF58E876 C1 [Buffington, M. L.] USDA ARS, Systemat Entomol Lab, Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20250 USA. [Copeland, R. S.] Int Ctr Insect Physiol & Ecol, Nairobi, Kenya. [Copeland, R. S.] Natl Museums Kenya, Div Invertebrate Zool, Nairobi, Kenya. RP Buffington, ML (reprint author), USDA ARS, Systemat Entomol Lab, Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20250 USA. EM matt.buffington@ars.usda.gov FU USDA Systematic Entomology Lab FX We thank Juliet Muriuki for help with fieldwork in Muhaka Forest. We are grateful to the former Director General of ICIPE, Dr Christian Borgemeister, for supporting our work on Kenyan insect biodiversity and its conservation. MLB was funded by the USDA Systematic Entomology Lab. We thank Mattias Forshage (Swedish Museum of Natural History) and John Noyes (The Natural History Museum) for comments that greatly improved this manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 30 TC 0 Z9 0 U1 0 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0022-2933 EI 1464-5262 J9 J NAT HIST JI J. Nat. Hist. PD NOV 25 PY 2015 VL 49 IS 43-44 BP 2597 EP 2607 DI 10.1080/00222933.2015.1042411 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CS5FL UT WOS:000362102600002 ER PT J AU Lichtenstein, AH AF Lichtenstein, Alice H. TI Fruits and Vegetables Get a Golden Halo Once Again Is There More to the Story? SO CIRCULATION LA English DT Editorial Material DE Editorials; cardiovascular diseases; diet; risk factors; vascular calcification ID CORONARY-HEART-DISEASE; CARDIOVASCULAR-DISEASE; BETA-CAROTENE; LUNG-CANCER; VITAMIN-E; RISK; CONSUMPTION; METAANALYSIS; COHORT; WOMEN C1 [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Alice.Lichtenstein@Tufts.edu NR 20 TC 0 Z9 0 U1 2 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD NOV 24 PY 2015 VL 132 IS 21 BP 1946 EP 1948 DI 10.1161/CIRCULATIONAHA.115.019326 PG 3 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA CX3DB UT WOS:000365574600003 PM 26503881 ER PT J AU Li, L Zheng, WG Zhu, YB Ye, HX Tang, BY Arendsee, ZW Jones, D Li, RR Ortiz, D Zhao, XF Du, CL Nettleton, D Scott, MP Salas-Fernandez, MG Yin, YH Wurtele, ES AF Li, Ling Zheng, Wenguang Zhu, Yanbing Ye, Huaxun Tang, Buyun Arendsee, Zebulun W. Jones, Dallas Li, Ruoran Ortiz, Diego Zhao, Xuefeng Du, Chuanlong Nettleton, Dan Scott, M. Paul Salas-Fernandez, Maria G. Yin, Yanhai Wurtele, Eve Syrkin TI QQS orphan gene regulates carbon and nitrogen partitioning across species via NF-YC interactions SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE QQS; NF-YC4; carbon allocation; nitrogen allocation; orphan ID TRANSCRIPTION FACTOR; ARABIDOPSIS-THALIANA; DNA-BINDING; RNA-SEQ; PROTEIN; EXPRESSION; METABOLISM; SUBUNITS; ALLOCATION; PATHWAYS AB The allocation of carbon and nitrogen resources to the synthesis of plant proteins, carbohydrates, and lipids is complex and under the control of many genes; much remains to be understood about this process. QQS (Qua-Quine Starch; At3g30720), an orphan gene unique to Arabidopsis thaliana, regulates metabolic processes affecting carbon and nitrogen partitioning among proteins and carbohydrates, modulating leaf and seed composition in Arabidopsis and soybean. Here the universality of QQS function in modulating carbon and nitrogen allocation is exemplified by a series of transgenic experiments. We show that ectopic expression of QQS increases soybean protein independent of the genetic background and original protein content of the cultivar. Furthermore, transgenic QQS expression increases the protein content of maize, a C4 species (a species that uses 4-carbon photosynthesis), and rice, a protein-poor agronomic crop, both highly divergent from Arabidopsis. We determine that QQS protein binds to the transcriptional regulator AtNF-YC4 (Arabidopsis nuclear factor Y, subunit C4). Overexpression of AtNF-YC4 in Arabidopsis mimics the QQS-overexpression phenotype, increasing protein and decreasing starch levels. NF-YC, a component of the NF-Y complex, is conserved across eukaryotes. The NF-YC4 homologs of soybean, rice, and maize also bind to QQS, which provides an explanation of how QQS can act in species where it does not occur endogenously. These findings are, to our knowledge, the first insight into the mechanism of action of QQS in modulating carbon and nitrogen allocation across species. They have major implications for the emergence and function of orphan genes, and identify a nontransgenic strategy for modulating protein levels in crop species, a trait of great agronomic significance. C1 [Li, Ling; Zheng, Wenguang; Zhu, Yanbing; Ye, Huaxun; Tang, Buyun; Arendsee, Zebulun W.; Jones, Dallas; Li, Ruoran; Yin, Yanhai; Wurtele, Eve Syrkin] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Li, Ling; Zheng, Wenguang; Wurtele, Eve Syrkin] Iowa State Univ, Ctr Metab Biol, Ames, IA 50011 USA. [Ortiz, Diego; Scott, M. Paul; Salas-Fernandez, Maria G.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Zhao, Xuefeng] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Du, Chuanlong; Nettleton, Dan] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Scott, M. Paul] ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Li, L (reprint author), Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. EM liling@iastate.edu; mash@iastate.edu OI Wurtele, Eve/0000-0003-1552-9495 FU National Science Foundation [MCB-0951170, IOS-1257631]; United Soybean Board [2287]; ISU Research Foundation; ISU Center for Metabolic Biology FX We thank Asheesh Singh, Mark Westgate, Walter Fehr, and Randy Shoemaker for helpful advice on soybean genetics and breeding; Walter Fehr for the elite soybean lines; Grace Welke for help with soybean crossing; Diane Luth, Marcy Main, Bronwyn Frame, and Kan Wang for introducing QQS into soybean, rice, and corn; Kent Berns for field management; Charles Hurburgh and Glen Rippke for near-infrared spectroscopy analysis of soybean and maize seed composition; and Jack Horner and Randall Den Adel for help using the microscopy equipment. We are grateful to Yan Xiong, Mark Stitt, and Basil Nikolau for helpful discussions. This research was supported by the National Science Foundation (MCB-0951170 to E.S.W. and L.L.; IOS-1257631 to Y.Y.), United Soybean Board (2287 to L.L.), ISU Research Foundation (L.L.), and ISU Center for Metabolic Biology (E.S.W.). NR 51 TC 2 Z9 2 U1 2 U2 28 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 24 PY 2015 VL 112 IS 47 BP 14734 EP 14739 DI 10.1073/pnas.1514670112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW7IY UT WOS:000365173100086 PM 26554020 ER PT J AU Porth, I Klapste, J McKown, AD La Mantia, J Guy, RD Ingvarsson, PK Hamelin, R Mansfield, SD Ehlting, J Douglas, CJ El-Kassaby, YA AF Porth, Ilga Klapste, Jaroslav McKown, Athena D. La Mantia, Jonathan Guy, Robert D. Ingvarsson, Par K. Hamelin, Richard Mansfield, Shawn D. Ehlting, Juergen Douglas, Carl J. El-Kassaby, Yousry A. TI Evolutionary Quantitative Genomics of Populus trichocarpa SO PLOS ONE LA English DT Article ID SINGLE NUCLEOTIDE POLYMORPHISMS; SPRUCE PICEA-SITCHENSIS; LOCAL ADAPTATION; TRAIT VARIATION; GENE FLOW; ARABIDOPSIS-THALIANA; POPULATION-STRUCTURE; NATURAL-POPULATIONS; BLACK COTTONWOOD; CLIMATE-CHANGE AB Forest trees generally show high levels of local adaptation and efforts focusing on understanding adaptation to climate will be crucial for species survival and management. Here, we address fundamental questions regarding the molecular basis of adaptation in undomesticated forest tree populations to past climatic environments by employing an integrative quantitative genetics and landscape genomics approach. Using this comprehensive approach, we studied the molecular basis of climate adaptation in 433 Populus trichocarpa (black cottonwood) genotypes originating across western North America. Variation in 74 field-assessed traits (growth, ecophysiology, phenology, leaf stomata, wood, and disease resistance) was investigated for signatures of selection (comparing Q(ST)-F-ST) using clustering of individuals by climate of origin (temperature and precipitation). 29,354 SNPs were investigated employing three different outlier detection methods and marker-inferred relatedness was estimated to obtain the narrow-sense estimate of population differentiation in wild populations. In addition, we compared our results with previously assessed selection of candidate SNPs using the 25 topographical units (drainages) across the P. trichocarpa sampling range as population groupings. Narrow-sense QST for 53% of distinct field traits was significantly divergent from expectations of neutrality (indicating adaptive trait variation); 2,855 SNPs showed signals of diversifying selection and of these, 118 SNPs (within 81 genes) were associated with adaptive traits (based on significant QST). Many SNPs were putatively pleiotropic for functionally uncorrelated adaptive traits, such as autumn phenology, height, and disease resistance. Evolutionary quantitative genomics in P. trichocarpa provides an enhanced understanding regarding the molecular basis of climate-driven selection in forest trees and we highlight that important loci underlying adaptive trait variation also show relationship to climate of origin. We consider our approach the most comprehensive, as it uncovers the molecular mechanisms of adaptation using multiple methods and tests. We also provide a detailed outline of the required analyses for studying adaptation to the environment in a population genomics context to better understand the species' potential adaptive capacity to future climatic scenarios. C1 [Porth, Ilga; Klapste, Jaroslav; McKown, Athena D.; La Mantia, Jonathan; Guy, Robert D.; Hamelin, Richard; El-Kassaby, Yousry A.] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. [Porth, Ilga] Univ Laval, Fac Foresterie Geog & Geomat, Dept Sci Bois & Foret, Quebec City, PQ G1V 0A6, Canada. [Klapste, Jaroslav] Czech Univ Life Sci, Dept Genet & Physiol Forest Trees, Prague 16521, Czech Republic. [La Mantia, Jonathan] USDA, Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. [Ingvarsson, Par K.] Umea Univ, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden. [Mansfield, Shawn D.] Univ British Columbia, Dept Wood Sci, Vancouver, BC V6T 1Z4, Canada. [Ehlting, Juergen] Univ Victoria, Dept Biol, Victoria, BC V8W 3N5, Canada. [Ehlting, Juergen] Univ Victoria, Ctr Forest Biol, Victoria, BC V8W 3N5, Canada. [Douglas, Carl J.] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. RP El-Kassaby, YA (reprint author), Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. EM y.el-kassaby@ubc.ca RI Ingvarsson, Par/G-2748-2010; Klapste, Jaroslav/B-6668-2016; El-Kassaby, Yousry/K-9856-2016; OI Ingvarsson, Par/0000-0001-9225-7521; Klapste, Jaroslav/0000-0001-5504-3735; El-Kassaby, Yousry/0000-0002-4887-8977; Porth, Ilga/0000-0002-9344-6348 FU Genome British Columbia Applied Genomics Innovation Program [103BIO]; Genome Canada Large-Scale Applied Research Project [168BIO] FX This work was supported by Genome British Columbia Applied Genomics Innovation Program (Project 103BIO) and Genome Canada Large-Scale Applied Research Project (Project 168BIO), funds to RDG, RCH, JE, SDM, CJD, and YE-K. NR 99 TC 0 Z9 0 U1 6 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 23 PY 2015 VL 10 IS 11 AR e0142864 DI 10.1371/journal.pone.0142864 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7AW UT WOS:000365853900043 PM 26599762 ER PT J AU Romanolo, KF Gorski, L Wang, S Lauzon, CR AF Romanolo, K. F. Gorski, L. Wang, S. Lauzon, C. R. TI Rapid Identification and Classification of Listeria spp. and Serotype Assignment of Listeria monocytogenes Using Fourier Transform-Infrared Spectroscopy and Artificial Neural Network Analysis SO PLOS ONE LA English DT Article ID FT-IR SPECTROSCOPY; UNITED-STATES; DISCRIMINATION; PATHOGENS; CULTURES; ASSAY AB The use of Fourier Transform-Infrared Spectroscopy (FT-IR) in conjunction with Artificial Neural Network software NeuroDeveloper (TM) was examined for the rapid identification and classification of Listeria species and serotyping of Listeria monocytogenes. A spectral library was created for 245 strains of Listeria spp. to give a biochemical fingerprint from which identification of unknown samples were made. This technology was able to accurately distinguish the Listeria species with 99.03% accuracy. Eleven serotypes of Listeria monocytogenes including 1/2a, 1/2b, and 4b were identified with 96.58% accuracy. In addition, motile and non-motile forms of Listeria were used to create a more robust model for identification. FT-IR coupled with NeuroDeveloper (TM) appear to be a more accurate and economic choice for rapid identification of pathogenic Listeria spp. than current methods. C1 [Romanolo, K. F.; Lauzon, C. R.] Calif State Univ East Bay, Dept Biol Sci, Hayward, CA 94542 USA. [Romanolo, K. F.; Gorski, L.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. [Wang, S.] Bruker Opt Inc, Fremont, CA USA. RP Lauzon, CR (reprint author), Calif State Univ East Bay, Dept Biol Sci, Hayward, CA 94542 USA. EM Carol.Lauzon@csueastbay.edu FU CSUPERB (CSU Program for Education and Research in Biotechnology) Joint Venture Program [FY 13/14]; Agricultural Research Service CRIS project [2030-42000-046-00D]; Bruker Optics, Inc FX This work was funded by a grant from CSUPERB (CSU Program for Education and Research in Biotechnology) Joint Venture Program FY 13/14 (https://www.calstate.edu/csuperb/) and funds from Agricultural Research Service CRIS project 2030-42000-046-00D. Bruker Optics, Inc provided support in the form of salary for SW, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the 'author contributions' section. NR 20 TC 1 Z9 1 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 23 PY 2015 VL 10 IS 11 AR e0143425 DI 10.1371/journal.pone.0143425 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7AW UT WOS:000365853900117 PM 26600423 ER PT J AU Stenfeldt, C Eschbaumer, M Pacheco, JM Rekant, SI Rodriguez, LL Arzt, J AF Stenfeldt, Carolina Eschbaumer, Michael Pacheco, Juan M. Rekant, Steven I. Rodriguez, Luis L. Arzt, Jonathan TI Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle SO PLOS ONE LA English DT Article ID DIRECT-CONTACT CHALLENGE; KIDNEY-CELL LINE; CARRIER STATE; SECRETORY IGA; SEROTYPE O; IN-VITRO; REPLICATION; INOCULATION; PERSISTENCE; INTERFERON AB A time-course pathogenesis study was performed to compare and contrast primary foot-and-mouth disease virus (FMDV) infection following simulated-natural (intra-nasopharyngeal) virus exposure of cattle that were non-vaccinated or vaccinated using a recombinant adenovirus-vectored FMDV vaccine. FMDV genome and infectious virus were detected during the initial phase of infection in both categories of animals with consistent predilection for the nasopharyngeal mucosa. A rapid progression of infection with viremia and widespread dissemination of virus occurred in non-vaccinated animals whilst vaccinated cattle were protected from viremia and clinical FMD. Analysis of micro-anatomic distribution of virus during early infection by lasercapture microdissection localized FMDV RNA to follicle-associated epithelium of the nasopharyngeal mucosa in both groups of animals, with concurrent detection of viral genome in nasopharyngeal MALT follicles in vaccinated cattle only. FMDV structural and non-structural proteins were detected in epithelial cells of the nasopharyngeal mucosa by immunomicroscopy 24 hours after inoculation in both non-vaccinated and vaccinated steers. Co-localization of CD11c(+)/MHC II+ cells with viral protein occurred early at primary infection sites in vaccinated steers while similar host-virus interactions were observed at later time points in non-vaccinated steers. Additionally, numerous CD8(+)/CD3(-) host cells, representing presumptive natural killer cells, were observed in association with foci of primary FMDV infection in the nasopharyngeal mucosa of vaccinated steers but were absent in non-vaccinated steers. Immunomicroscopic evidence of an activated antiviral response at primary infection sites of vaccinated cattle was corroborated by a relative induction of interferon -alpha, -beta, -gamma and -lambda mRNA in micro-dissected samples of nasopharyngeal mucosa. Although vaccination protected cattle from viremia and clinical FMD, there was subclinical infection of epithelial cells of the nasopharyngeal mucosa that could enable shedding and long-term persistence of infectious virus. Additionally, these data indicate different mechanisms within the immediate host response to infection between non-vaccinated and vaccinated cattle. C1 [Stenfeldt, Carolina; Eschbaumer, Michael; Pacheco, Juan M.; Rekant, Steven I.; Rodriguez, Luis L.; Arzt, Jonathan] ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. [Stenfeldt, Carolina; Eschbaumer, Michael; Rekant, Steven I.] PIADC Res Participat Program, Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. RP Arzt, J (reprint author), ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. EM Jonathan.Arzt@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201; Stenfeldt, Carolina/0000-0002-2074-3886; Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQPM-13-X-00131]; US Department of Agriculture - Agricultural Research Service, Current Research Information System Project [1940-32000-057-00D]; Plum Island Animal Disease Center Research Participation Program fellowship FX This research was funded by an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security (award number HSHQPM-13-X-00131). Additional funding came from US Department of Agriculture - Agricultural Research Service, Current Research Information System Project 1940-32000-057-00D. CS, ME and SIR are recipients of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE, www.orau.org) through an interagency agreement with the US Department of Energy. None of the funding sources had influence upon design or performance of experimental studies, interpretation of results or writing of the manuscript. NR 51 TC 6 Z9 7 U1 4 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 23 PY 2015 VL 10 IS 11 AR e0143666 DI 10.1371/journal.pone.0143666 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7AW UT WOS:000365853900155 PM 26599543 ER PT J AU Summers, CF Gulliford, CM Carlson, CH Lillis, JA Carlson, MO Cadle-Davidson, L Gent, DH Smart, CD AF Summers, Carly F. Gulliford, Colwyn M. Carlson, Craig H. Lillis, Jacquelyn A. Carlson, Maryn O. Cadle-Davidson, Lance Gent, David H. Smart, Christine D. TI Identification of Genetic Variation between Obligate Plant Pathogens Pseudoperonospora cubensis and P-humuli Using RNA Sequencing and Genotyping-By-Sequencing SO PLOS ONE LA English DT Article ID DOWNY MILDEW; DNA-SEQUENCES; CAUSAL AGENT; MATING-TYPE; OOMYCETE; GENOME; SEQ; CUCURBITS; SNPS; SPECIALIZATION AB RNA sequencing (RNA-seq) and genotyping-by-sequencing (GBS) were used for single nucleotide polymorphism (SNP) identification from two economically important obligate plant pathogens, Pseudoperonospora cubensis and P. humuli. Twenty isolates of P. cubensis and 19 isolates of P. humuli were genotyped using RNA-seq and GBS. Principle components analysis (PCA) of each data set showed genetic separation between the two species. Additionally, results supported previous findings that P. cubensis isolates from squash are genetically distinct from cucumber and cantaloupe isolates. A PCA-based procedure was used to identify SNPs correlated with the separation of the two species, with 994 and 4,231 PCA-correlated SNPs found within the RNA-seq and GBS data, respectively. The corresponding unigenes (n = 800) containing these potential species-specific SNPs were then annotated and 135 putative pathogenicity genes, including 3 effectors, were identified. The characterization of genes containing SNPs differentiating these two closely related downy mildew species may contribute to the development of improved detection and diagnosis strategies and improve our understanding of host specificity pathways. C1 [Summers, Carly F.; Carlson, Maryn O.; Smart, Christine D.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. [Gulliford, Colwyn M.] Cornell Univ, Cornell Lab Accelerator Based Sci & Educ, Ithaca, NY USA. [Carlson, Craig H.] Cornell Univ, Hort Sect, Sch Integrat Plant Sci, Geneva, NY USA. [Lillis, Jacquelyn A.; Cadle-Davidson, Lance] USDA ARS, Grape Genet Res Unit, Geneva, NY USA. [Gent, David H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Smart, CD (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. EM cds14@cornell.edu FU United States Department of Agriculture Organic Research and Extension Initiative (NIFA) [2012-51300-20006]; National Institutes of Health [1S10OD010693-01]; NSF Graduate Research Fellowship [DGE-1144153] FX Funding for this work was provided through the United States Department of Agriculture Organic Research and Extension Initiative (http://nifa.usda.gov/funding-opportunity/organic-agriculture-research-a nd-extension-initiative; NIFA contract no. 2012-51300-20006) and National Institutes of Health (http://www.nih.gov/; grant 1S10OD010693-01). Funding for C.F. Summers was also provided through an NSF Graduate Research Fellowship (www.nsfgrfp.org; DGE-1144153). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 1 Z9 1 U1 7 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 23 PY 2015 VL 10 IS 11 AR e0143665 DI 10.1371/journal.pone.0143665 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CX7AW UT WOS:000365853900154 PM 26599440 ER PT J AU Li, BQ Feng, CH Wang, MR Hu, LY Volk, G Wang, QC AF Li, Bai-Quan Feng, Chao-Hong Wang, Min-Rui Hu, Ling-Yun Volk, Gayle Wang, Qiao-Chun TI Recovery patterns, histological observations and genetic integrity in Malus shoot tips cryopreserved using droplet-vitrification and encapsulation-dehydration procedures SO JOURNAL OF BIOTECHNOLOGY LA English DT Article DE Droplet-vitrification; Encapsulation-dehydration; Genetic stability; Histological observation; Malus; Shoot tips ID PATHOGEN ERADICATION; LIQUID-NITROGEN; APPLE BUDS; STABILITY; POTATO; PRECULTURE; RESOURCES; MERISTEMS; CULTURES; GROWTH AB A droplet-vitrification procedure is described for cryopreservation of Mains shoot tips. Survival patterns, recovery types, histological observations, and genetic integrity were compared for Malus shoot tips cryopreserved using this droplet-vitrification procedure and an encapsulation-dehydration procedure that was previously reported by us. In both procedures, three types of shoot tip recovery were observed following cryopreservation: callus formation without shoot regrowth, leaf formation without shoot regrowth, and shoot regrowth. Three categories of histological observations were also identified in cross-sections of shoot tips recovered after cryopreservation using the two cryogenic procedures. In category 1, almost all of the cells (94-95%) in the apical dome (AD) were damaged or killed and only some cells (30-32%) in the leaf primordia (LPs) survived. In category 2, only a few cells (18-20%) in the AD and some cells (30-31%) in the LPs survived. In category 3, majority of the cells (60-62%) in the AD and some cells (30-33%) in the LPs survived. These data suggest that shoot regrowth is correlated to the presence of a majority of surviving cells in the AD after liquid nitrogen exposure. No polymorphic bands were detected by inter-simple sequence repeats or by random amplified polymorphic DNA assessments, and ploidy levels analyzed by flow cytometry were unchanged when plants recovered after cryoexposure were compared to controls. The droplet-vitrification procedure appears to be robust since seven genotypes representing four Malus species and one hybrid recovered shoots following cryopreservation. Mean shoot regrowth levels of these seven genotypes were 48% in the droplet-vitrification method, which were lower than those (61%) in the encapsulation-dehydration procedure reported in our previous study, suggesting the latter may be preferred for routine cryobanking applications for Malus shoot tips. (C) 2015 Elsevier B.V. All rights reserved. C1 [Li, Bai-Quan; Feng, Chao-Hong; Wang, Min-Rui; Hu, Ling-Yun; Wang, Qiao-Chun] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Volk, Gayle] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Wang, QC (reprint author), Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. EM qiaochunwang@nwsuaf.edu.cn FU Department of Science & Technology of Shaanxi Province [2014KTCL02-05] FX The authors acknowledge financial support from the fund provided by Department of Science & Technology of Shaanxi Province (2014KTCL02-05). NR 48 TC 2 Z9 2 U1 5 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1656 EI 1873-4863 J9 J BIOTECHNOL JI J. Biotechnol. PD NOV 20 PY 2015 VL 214 BP 182 EP 191 DI 10.1016/j.jbiotec.2015.09.030 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CX0CQ UT WOS:000365364900035 PM 26432336 ER PT J AU Hermance, JF Augustine, DJ Derner, JD AF Hermance, John F. Augustine, David J. Derner, Justin D. TI Quantifying characteristic growth dynamics in a semi-arid grassland ecosystem by predicting short-term NDVI phenology from daily rainfall: a simple four parameter coupled-reservoir model SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID FORAGE PRODUCTION; AVHRR NDVI; PRODUCTIVITY RESPONSES; HERBACEOUS BIOMASS; VEGETATION INDEXES; PLANT-RESPONSES; SOIL-MOISTURE; TIME-SERIES; NOAA AVHRR; PRECIPITATION AB Predicting impacts on phenology of the magnitude and seasonal timing of rainfall pulses in water-limited grassland ecosystems concerns ecologists, climate scientists, hydrologists, and a variety of stakeholders. This report describes a simple, effective procedure to emulate the seasonal response of grassland biomass, represented by the satellite-based normalized difference vegetation index (NDVI), to daily rainfall. The application is a straightforward adaptation of a staged linear reservoir that simulates the pulse-like entry of rainwater into the soil and its redistribution as soil moisture, the uptake of water by plant roots, short-term biomass development, followed by the subsequent transpiration of water through foliage. The algorithm precludes the need for detailed, site specific information on soil moisture dynamics, plant species, and the local hydroclimate, while providing a direct link between discrete rainfall events and consequential biomass responses throughout the growing season. We applied the algorithm using rainfall data from the Central Plains Experimental Range to predict vegetation growth dynamics in the semi-arid shortgrass steppe of North America. The mean annual rainfall is 342mm, which is strongly bifurcated into a dominantly wet' season, where during the three wettest months (May, June and July) the mean monthly rainfall is approximately 55mm month(-1); and a dry' season, where during the three driest months (December, January and February), the mean monthly rainfall is approximately 7mm month(-1). NDVI data from the Moderate Resolution Imaging Spectroradiometer (MODIS) MOD13Q1 16day, 250 mx250m product were used as a proxy for grassland phenology for the period-of-record 2000-2013. Allowing for temporal changes in basic parameters of the response function over the growing season, the predicted response of the model tracks the observed NDVI metric with correlation coefficients exceeding 0.92. A two-stage series reservoir is preferred, whereby the characteristic time for transfer of a rainfall event to the peak response of NDVI decreases from 24days (early growing season) to 12days (late growing season), while the efficiency of a given volume of rainfall to produce a correspondingly similar amount of aboveground biomass decreases by a factor of 40% from April to October. Behaviours of the characteristic time of greenup and loss of rainfall efficiency with progression of the growing season are consistent with physiological traits of cool-season C-3 grasses versus warm-season C-4 grasses, and with prior research suggesting that early season production by C-3 grasses is more responsive to a given amount of precipitation than mid-summer growth of C-4 shortgrasses. Our model explains >90% of seasonal biomass dynamics. We ascribe a systematic underprediction of observed early season greenup following drought years to a lagged or legacy' effect, as soil inorganic nitrogen, accumulated during drought, becomes available for future plant uptake. C1 [Hermance, John F.] Brown Univ, Dept Earth Environm & Planetary Sci, Providence, RI 02912 USA. [Augustine, David J.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. RP Hermance, JF (reprint author), Brown Univ, Dept Earth Environm & Planetary Sci, Providence, RI 02912 USA. EM John_Hermance@Brown.Edu FU Shortgrass Steppe Long Term Ecological Research (LTER) project (National Science Foundation) [0217631] FX Funding for the ground-based measurements was provided by the Shortgrass Steppe Long Term Ecological Research (LTER) project (National Science Foundation Grant No. [0217631]). NR 70 TC 2 Z9 2 U1 8 U2 31 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD NOV 20 PY 2015 VL 36 IS 22 BP 5637 EP 5663 DI 10.1080/01431161.2015.1103916 PG 27 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA CW6DF UT WOS:000365087000006 ER PT J AU Mueller-Warrant, GW Whittaker, GW AF Mueller-Warrant, George W. Whittaker, Gerald W. TI Distorted spatial warping to compress large, high-resolution rasters for remote-sensing classification SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID LANDSCAPE; INDEXES AB Decisions made early in the data preparation phases of remote-sensing classification projects set fundamental limits on the value to society of the final products. The often-used approach of degrading/down-sampling high-resolution (e.g. 1m pixel size) imagery to match lower-resolution data (e.g. Landsat 30m) through averaging or majority-rule solves the problem of aligning pixels across bands of differing resolution, but does so by forgoing all ability to detect features smaller than 30m in addition to potentially discarding up to 99% of the information content of the high-resolution data. The alternative of up-sampling coarser-resolution data into smaller-sized synthetic pixels creates its own set of problems, including potentially enormous file sizes, likely absence of meaningful variation over small spatial scales (which may generate matrix singularities fatal to the maximum likelihood classifier), and no assurance of meaningful improvement in classification accuracy despite guaranteed increases in computational time and resource requirements. We propose a new warped space compression technique' as a variation of vector quantization that analyses local variability in the finest-resolution data available to define acceptable pixel-based neighbourhood (NxN) sizes over which data can be averaged while minimizing overall information loss. Alternative neighbourhoods are aligned so that nine smaller ones nest within each progressively larger one as 3x3 squares, resulting in local data compression options of 3x3 (ninefold), 9x9 (81-fold), 27x27 (729-fold), and 81x81 (6561-fold). Our transformation process to warped space' created spatially distorted images with jagged east edges and little visually discernible relationship to the original data. We achieved compressions of 48- to 138-fold in disc storage and 292- to 785-fold in actual numbers of non-null pixels through our choice of cut-off values for accepting 3x3, 9x9, 27x27, or 81x81 neighbourhoods of tolerable variability, while otherwise retaining full (1m) resolution data in regions three cells wide by three cells high. Medium-resolution data (e.g. Landsat 30m) can be translated into the warped space defined by high-resolution data and composited with it for conducting remote-sensing classifications. When applied to a 71-band, 55-class remote-sensing classification of a 25,500 km(2) region centred on the Willamette Valley of Oregon, USA, classification accuracy increased from 64.4% in normal space to 71.3% in warped space. Unsupervised classification in warped space identified several additional categories that could be appended to the 55 existing ground-truth classes, leading to further increases in accuracy. Warped-space compression may be particularly beneficial for ecological studies where it could maintain high resolution in features of interest such as riparian buffers without creating exorbitantly large data files. C1 [Mueller-Warrant, George W.; Whittaker, Gerald W.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Mueller-Warrant, GW (reprint author), ARS, USDA, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM George.Mueller-Warrant@ARS.USDA.GOV NR 32 TC 0 Z9 0 U1 4 U2 4 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD NOV 20 PY 2015 VL 36 IS 22 BP 5664 EP 5689 DI 10.1080/01431161.2015.1103917 PG 26 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA CW6DF UT WOS:000365087000007 ER PT J AU Kurtzman, CP AF Kurtzman, Cletus P. TI Identification of food and beverage spoilage yeasts from DNA sequence analyses SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article; Proceedings Paper CT 24th International ICFMH Conference from Single Cells to Functions of Consortia in Food Microbiology CY SEP 01-01, 2014 CL Nantes, FRANCE DE Yeasts; Food spoilage; Species identification; Taxonomy; Systematics ID DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; GRADIENT GEL-ELECTROPHORESIS; INTERNAL TRANSCRIBED SPACER; HUMAN PATHOGENIC YEASTS; IN-SITU HYBRIDIZATION; NUCLEIC-ACID PROBES; LARGE-SUBUNIT RDNA; MALDI-TOF MS; RAPID IDENTIFICATION AB Detection, identification and classification of yeasts have undergone major changes in the last decade and a half following application of gene sequence analyses and genome comparisons. Development of a database (barcode) of easily determined DNA sequences from domains 1 and 2 (D1/D2) of the nuclear large subunit rRNA gene and from ITS now permits many laboratories to identify species quickly and accurately, thus replacing the laborious and often inaccurate phenotypic tests previously used. Phylogenetic analysis of gene sequences has resulted in a major revision of yeast systematics resulting in redefinition of nearly all genera. This new understanding of species relationships has prompted a change of rules for naming and classifying yeasts and other fungi, and these new rules are presented in the recently implemented International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). The use of molecular methods for species identification and the impact of Code changes on classification will be discussed, especially in the context of food and beverage spoilage yeasts. Published by Elsevier B.V. C1 ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 72 TC 2 Z9 2 U1 10 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD NOV 20 PY 2015 VL 213 SI SI BP 71 EP 78 DI 10.1016/j.ijfoodmicro.2015.05.023 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CV4VX UT WOS:000364265700009 PM 26051959 ER PT J AU Biswas, A Appell, M Liu, ZS Cheng, HN AF Biswas, Atanu Appell, Michael Liu, Zengshe Cheng, H. N. TI Microwave-assisted synthesis of cyclodextrin polyurethanes SO CARBOHYDRATE POLYMERS LA English DT Article DE Cyclodextrin; TDI; MDI; HDI; Polyurethane; Microwave; NMR ID DIISOCYANATE; SPECTROSCOPY; REMOVAL AB Cyclodextrin (CD) has often been incorporated into polyurethanes in order to facilitate its use in encapsulation or removal of organic species for various applications. In this work a microwave-assisted method has been developed to produce polyurethanes consisting of alpha-, beta-, and gamma-CD and three common diisocyanates. As compared to conventional heating, this new synthetic method saves energy, significantly reduces reaction time, and gets similar or improved yield. The reaction products have been fully characterized with C-13, H-1, and two-dimensional NMR spectroscopy. With suitable stoichiometry of starting CD and diisocyanate, the resulting CD polyurethane is organic-soluble and water-insoluble and is shown to remove Nile red dye and phenol from water. Possible applications include the removal of undesirable materials from process streams, toxic compounds from the environment, and encapsulation of color or fragrance molecules. Published by Elsevier Ltd. C1 [Biswas, Atanu; Appell, Michael; Liu, Zengshe] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Cheng, H. N.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Biswas, A (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815N Univ St, Peoria, IL 61604 USA. EM atanu.biswas@ars.usda.gov; hn.cheng@ars.usda.gov NR 30 TC 7 Z9 7 U1 10 U2 55 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD NOV 20 PY 2015 VL 133 BP 74 EP 79 DI 10.1016/j.carbpol.2015.06.044 PG 6 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA CS2SC UT WOS:000361920900011 PM 26344257 ER PT J AU Yue, YY Han, JQ Han, GP Zhang, QG French, AD Wu, QL AF Yue, Yiying Han, Jingquan Han, Guangping Zhang, Quanguo French, Alfred D. Wu, Qinglin TI Characterization of cellulose I/II hybrid fibers isolated from energycane bagasse during the delignification process.: Morphology, crystallinity and percentage estimation SO CARBOHYDRATE POLYMERS LA English DT Article DE Cellulose; Hybrid fibers; Defibrillation; Transformation; Crystallinity ID X-RAY-DIFFRACTION; SUGARCANE BAGASSE; THERMAL CHARACTERIZATION; POLYMER COMPOSITES; C-13 NMR; LIGNIN; SIZE; NANOPARTICLES; SPECTROSCOPY; DISSOLUTION AB Cellulose I, cellulose II and cellulose I/II hybrid fibers were prepared from energycane bagasse using NaOH and NaClO2 treatments. The definitive defibrillation effect with an average width of 12 5 pm was observed for the fibers treated with 20 wt% NaOH for 10 h and NaClO2 for 2 h. The ribbon shaped cellulose I fibers were converted to a swollen state with a rougher surface by 20 wt% NaOH treatment for 10 h. The percentage of cellulose I decreased from 100% to 5%, and the corresponding Cl values increased from 58.2% to 68.8% during the conversion from cellulose I to II. After further NaClO2 treatment, the Cl values were decreased because of partial destruction of hydrogen bond network. XRD, NMR and FTIR results present the same trend in the degree of crystallization for all the samples. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Yue, Yiying; Wu, Qinglin] Louisiana State Univ, Sch Renewable Nat Resources, Ctr Agr, Baton Rouge, LA 70803 USA. [Han, Jingquan] Nanjing Forestry Univ, Coll Mat Sci & Engn, Nanjing 210037, Jiangsu, Peoples R China. [Han, Guangping] Northeast Forestry Univ, Key Lab Biobased Mat Sci & Technol, Minist Educ, Harbin 150040, Peoples R China. [Zhang, Quanguo] Henan Agr Univ, Key Lab New Mat & Facil Rural Renewable Energy, Minist Agr, Zhengzhou 45002, Henan, Peoples R China. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Han, GP (reprint author), Northeast Forestry Univ, Key Lab Biobased Mat Sci & Technol, Minist Educ, Harbin 150040, Peoples R China. EM guangpingh@hotmail.com; wuqing@lsu.edu FU State Forestry Bureau 948 project [2013-4-11]; LSU School of Renewable Natural Resources FX The authors gratefully acknowledge the financial support from the State Forestry Bureau 948 project (Grant No. 2013-4-11) and from the LSU School of Renewable Natural Resources. NR 52 TC 19 Z9 19 U1 3 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD NOV 20 PY 2015 VL 133 BP 438 EP 447 DI 10.1016/j.carbpol.2015.07.058 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA CS2SC UT WOS:000361920900054 PM 26344300 ER PT J AU Dong, H Sliozberg, YR Snyder, JF Steele, J Chantawansri, TL Orlicki, JA Walck, SD Reiner, RS Rudie, AW AF Dong, Hong Sliozberg, Yelena R. Snyder, James F. Steele, Joshua Chantawansri, Tanya L. Orlicki, Joshua A. Walck, Scott D. Reiner, Richard S. Rudie, Alan W. TI Highly Transparent and Toughened Poly(methyl methacrylate) Nanocomposite Films Containing Networks of Cellulose Nanofibrils SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE cellulose nanofibrils; poly(methyl methacrylate); nanocomposites; interfacial interactions; mechanical properties; coarse-grained simulation ID MOLECULAR-DYNAMICS; CARBON NANOTUBES; POLYMER; NANOPARTICLES; SIMULATIONS; COMPOSITES; BEHAVIOR; ACID AB Cellulose nanofibrils (CNFs) are a class of cellulosic nanomaterials with high aspect ratios that can be extracted from various natural sources. Their highly crystalline structures provide the nanofibrils with excellent mechanical and thermal properties. The main challenges of CNFs in nanocomposite applications are associated with their high hydrophilicity, which makes CNFs incompatible with hydrophobic polymers. In this study, highly transparent and toughened poly(methyl methacrylate) (PMMA) nanocomposite films were prepared using various percentages of CNFs covered with surface carboxylic acid groups (CNF-COOH). The surface groups make the CNFs interfacial interaction with PMMA favorable, which facilitate the homogeneous dispersion of the hydrophilic nanofibrils in the hydrophobic polymer and the formation of a percolated network of nanofibrils. The controlled dispersion results in high transparency of the nanocomposites. Mechanical analysis of the resulting films demonstrated that a low percentage loading of CNF-COOH worked as effective reinforcing agents, yielding more ductile and therefore tougher films than the neat PMMA film. Toughening mechanisms were investigated through coarse-grained simulations, where the results demonstrated that a favorable polymer-nanofibril interface together with percolation of the nanofibrils, both facilitated through hydrogen bonding interactions, contributed to the toughness improvement in these nanocomposites. C1 [Dong, Hong; Sliozberg, Yelena R.; Snyder, James F.; Steele, Joshua; Chantawansri, Tanya L.; Orlicki, Joshua A.; Walck, Scott D.] US Army, Res Lab, Macromol Sci & Technol Branch, Aberdeen Proving Ground, MD 21005 USA. [Dong, Hong; Sliozberg, Yelena R.; Walck, Scott D.] TKC Global Solut LLC, Aberdeen, MD 21005 USA. [Reiner, Richard S.; Rudie, Alan W.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Dong, H (reprint author), US Army, Res Lab, Biotechnol Branch, Adelphi, MD 20783 USA. EM hong.dong.ctr@mail.mil FU U.S. Army Research Laboratory [W911QX-14-C-0016]; U.S. Army Research Laboratory FX The research reported in this document was performed in connection with contract/instrument W911QX-14-C-0016 with the U.S. Army Research Laboratory. J. Steele was supported in part by an appointment to the Postgraduate Research Participation Program at the U.S. Army Research Laboratory administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and USARL. The authors thank Dr. Randy Mrozek, Dr. Erich Bain, and Mr. Eugene Napadensky at U.S. Army Research Laboratory for useful discussions. NR 32 TC 6 Z9 6 U1 15 U2 60 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD NOV 18 PY 2015 VL 7 IS 45 BP 25464 EP 25472 DI 10.1021/acsami.5b08317 PG 9 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA CW6ZW UT WOS:000365148600057 PM 26513136 ER PT J AU Oppert, B Guedes, RNC Aikins, MJ Perkin, L Chen, Z Phillips, TW Zhu, KY Opit, GP Hoon, K Sun, YM Meredith, G Bramlett, K Hernandez, NS Sanderson, B Taylor, MW Dhingra, D Blakey, B Lorenzen, M Adedipe, F Arthur, F AF Oppert, Brenda Guedes, Raul N. C. Aikins, Michael J. Perkin, Lindsey Chen, Zhaorigetu Phillips, Thomas W. Zhu, Kun Yan Opit, George P. Hoon, Kelly Sun, Yongming Meredith, Gavin Bramlett, Kelli Hernandez, Natalie Supunpong Sanderson, Brian Taylor, Madison W. Dhingra, Dalia Blakey, Brandon Lorenzen, Marce Adedipe, Folukemi Arthur, Frank TI Genes related to mitochondrial functions are differentially expressed in phosphine-resistant and -susceptible Tribolium castaneum SO BMC GENOMICS LA English DT Article DE Anti-diuretic peptide; Cytochrome P450; Deltamethrin; Dihydrolipoamide dehydrogenase; Fumigants; Insecticide resistance; Gene expression; Phosphine resistance; Red flour beetle; RNA-Seq; Stored product pests; Tribolium castaneum ID STORED-PRODUCT INSECTS; ANOPHELES-GAMBIAE; TOXIC ACTION; CYSTEINE; PROTEIN; STRAIN; INJURY AB Background: Phosphine is a valuable fumigant to control pest populations in stored grains and grain products. However, recent studies indicate a substantial increase in phosphine resistance in stored product pests worldwide. Results: To understand the molecular bases of phosphine resistance in insects, we used RNA-Seq to compare gene expression in phosphine-resistant and susceptible laboratory populations of the red flour beetle, Tribolium castaneum. Each population was evaluated as either phosphine-exposed or no phosphine (untreated controls) in triplicate biological replicates (12 samples total). Pairwise analysis indicated there were eight genes differentially expressed between susceptible and resistant insects not exposed to phosphine (i.e., basal expression) or those exposed to phopshine (>8-fold expression and 90 % C.I.). However, 214 genes were differentially expressed among all four treatment groups at a statistically significant level (ANOVA, p < 0.05). Increased expression of 44 cytochrome P450 genes was found in resistant vs. susceptible insects, and phosphine exposure resulted in additional increases of 21 of these genes, five of which were significant among all treatment groups (p < 0.05). Expression of two genes encoding anti-diruetic peptide was 2- to 8-fold reduced in phosphine-resistant insects, and when exposed to phosphine, expression was further reduced 36- to 500-fold compared to susceptible. Phosphine-resistant insects also displayed differential expression of cuticle, carbohydrate, protease, transporter, and many mitochondrial genes, among others. Gene ontology terms associated with mitochondrial functions (oxidation biological processes, monooxygenase and catalytic molecular functions, and iron, heme, and tetrapyyrole binding) were enriched in the significantly differentially expressed dataset. Sequence polymorphism was found in transcripts encoding a known phosphine resistance gene, dihydrolipoamide dehydrogenase, in both susceptible and resistant insects. Phosphine-resistant adults also were resistant to knockdown by the pyrethroid deltamethrin, likely due to the increased cytochrome P450 expression. Conclusions: Overall, genes associated with the mitochondria were differentially expressed in resistant insects, and these differences may contribute to a reduction in overall metabolism and energy production and/or compensation in resistant insects. These data provide the first gene expression data on the response of phosphine-resistant and -susceptible insects to phosphine exposure, and demonstrate that RNA-Seq is a valuable tool to examine differences in insects that respond differentially to environmental stimuli. C1 [Oppert, Brenda; Perkin, Lindsey; Arthur, Frank] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Guedes, Raul N. C.] Univ Fed Vicosa, Dept Entomol, Vicosa, MG, Brazil. [Aikins, Michael J.; Chen, Zhaorigetu; Phillips, Thomas W.; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Opit, George P.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Hoon, Kelly; Sun, Yongming; Meredith, Gavin; Bramlett, Kelli; Hernandez, Natalie Supunpong; Sanderson, Brian; Taylor, Madison W.; Dhingra, Dalia; Blakey, Brandon] LifeTechnol, Carlsbad, CA USA. [Lorenzen, Marce; Adedipe, Folukemi] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. RP Oppert, B (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. EM brenda.oppert@ars.usda.gov FU KAES; Australian Plant Biosecurity-Cooperative Research Center FX We would like to thank Tom Morgan for excellent technical support. This manuscript is contribution no. 14-082-J from the Kansas Agricultural Experiment Station (KAES), Kansas State University, Manhattan, Kansas. Partial financial support for MJA, TWP, and ZC came from the KAES and the Australian Plant Biosecurity-Cooperative Research Center. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture and Universities. All associated U.S. institutions are equal opportunity providers and employers. NR 26 TC 1 Z9 1 U1 7 U2 29 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 18 PY 2015 VL 16 AR 968 DI 10.1186/s12864-015-2121-0 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CW8YE UT WOS:000365284600003 PM 26582239 ER PT J AU Ramsey, JS Johnson, RS Hoki, JS Kruse, A Mahoney, J Hilf, ME Hunter, WB Hall, DG Schroeder, FC MacCoss, MJ Cilia, M AF Ramsey, John S. Johnson, Richard S. Hoki, Jason S. Kruse, Angela Mahoney, Jaclyn Hilf, Mark E. Hunter, Wayne B. Hall, David G. Schroeder, Frank C. MacCoss, Michael J. Cilia, Michelle TI Metabolic Interplay between the Asian Citrus Psyllid and Its Profftella Symbiont: An Achilles' Heel of the Citrus Greening Insect Vector SO PLOS ONE LA English DT Article ID CANDIDATUS LIBERIBACTER ASIATICUS; HEMIPTERA PSYLLIDAE; STATISTICAL-MODEL; DISEASE; IDENTIFICATIONS; BIOSYNTHESIS; INFECTION; DATABASE; GENOME AB 'Candidatus Liberibacter asiaticus' (CLas), the bacterial pathogen associated with citrus greening disease, is transmitted by Diaphorina citri, the Asian citrus psyllid. Interactions among D. citri and its microbial endosymbionts, including 'Candidatus Profftella armatura', are likely to impact transmission of CLas. We used quantitative mass spectrometry to compare the proteomes of CLas(+) and CLas(-) populations of D. citri, and found that proteins involved in polyketide biosynthesis by the endosymbiont Profftella were up-regulated in CLas(+) insects. Mass spectrometry analysis of the Profftella polyketide diaphorin in D. citri metabolite extracts revealed the presence of a novel diaphorin-related polyketide and the ratio of these two polyketides was changed in CLas(+) insects. Insect proteins differentially expressed between CLas(+) and CLas(-) D. citri included defense and immunity proteins, proteins involved in energy storage and utilization, and proteins involved in endocytosis, cellular adhesion, and cytoskeletal remodeling which are associated with microbial invasion of host cells. Insight into the metabolic interdependence between the insect vector, its endosymbionts, and the citrus greening pathogen reveals novel opportunities for control of this disease, which is currently having a devastating impact on citrus production worldwide. C1 [Ramsey, John S.; Hoki, Jason S.; Kruse, Angela; Mahoney, Jaclyn; Schroeder, Frank C.; Cilia, Michelle] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Ramsey, John S.; Mahoney, Jaclyn; Cilia, Michelle] USDA ARS, Robert W Holley Ctr Agr & Hlth, Emerging Pests & Pathogens Res Unit, Ithaca, NY 14853 USA. [Johnson, Richard S.; MacCoss, Michael J.] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Hoki, Jason S.; Schroeder, Frank C.] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY USA. [Kruse, Angela; Cilia, Michelle] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY USA. [Hilf, Mark E.] USDA ARS, US Hort Res Lab, Subtrop Plant Pathol Res Unit, Ft Pierce, FL USA. [Hunter, Wayne B.; Hall, David G.] USDA ARS, US Hort Res Lab, Subtrop Insects & Hort Res Unit, Ft Pierce, FL USA. RP Cilia, M (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM mic68@cornell.edu OI Schroeder, Frank/0000-0002-4420-0237 FU Citrus Research Board [5300-155, 5300-163]; USDA ARS project [6034-22320-001-00, 8062-22000-021-00] FX This study was supported by Citrus Research Board: http://citrusresearch.com/; Grant numbers: 5300-155, 5300-163, USDA ARS project numbers: 6034-22320-001-00 and 8062-22000-021-00. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 30 TC 4 Z9 4 U1 5 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 18 PY 2015 VL 10 IS 11 AR e0140826 DI 10.1371/journal.pone.0140826 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW7CD UT WOS:000365154600008 PM 26580079 ER PT J AU Fu, YW Zhang, QZ Xu, DH Wang, B Liang, JH Lin, DJ AF Fu, Yao-Wu Zhang, Qi-Zhong Xu, De-Hai Wang, Bin Liang, Jing-Han Lin, De-Jie TI Cynatratoside-C efficacy against theronts of Ichthyophthirius multifiliis, and toxicity tests on grass carp and mammal blood cells SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Cynatratoside-C; Cynanchum atratum; Ctenopharyngodon idellus; Antiparasitic activity; Toxicity; Ichthyophthirius multifiliis ID GOLDFISH CARASSIUS-AURATUS; IN-VITRO; ANTIPARASITIC EFFICACY; PSORALEA-CORYLIFOLIA; CYNANCHUM-ATRATUM; MEDICINAL-PLANTS; CHANNEL CATFISH; MORUS-ALBA; ROOT BARK; FISH AB Infection by Ichthyophthirius multifiliis, a ciliated protozoan parasite, results in high fish mortality and causes severe economic losses in aquaculture. To find new, efficient anti-I. multifiliis agents, cynatratoside-C was isolated from Cynanchum atratum by bioassay-guided fractionation in a previous study. The present study investigated the anti-theront activity, determined the toxicity of cynatratoside-C to grass carp Ctenopharyngodon idellus and mammalian blood cells, and evaluated the protection of cynatratoside-C against I. multifiliis theront infection in grass carp. Results showed that all theronts were killed by 0.25 mg l(-1) of cynatratoside-C in 186.7 +/- 5.8 min. Cynatratoside-C at 0.25 mg l(-1) was effective in treating infected grass carp and protecting naive fish from I. multifiliis infestation. The 96 h median lethal concentration (LC50) of cynatratoside-C to grass carp and 4 h median effective concentration (EC50) of cynatratoside-C to theront were 46.8 and 0.088 mg l(-1), respectively. In addition, the hemolysis assay demonstrated that cynatratoside-C had no cytotoxicity to rabbit red blood cells. Therefore, cynatratoside-C could be a safe and effective potential parasiticide for controlling I. multifiliis. C1 [Fu, Yao-Wu; Zhang, Qi-Zhong; Wang, Bin; Liang, Jing-Han; Lin, De-Jie] Jinan Univ, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Inst Hydrobiol,Minist Educ, Guangzhou 510632, Guangdong, Peoples R China. [Xu, De-Hai] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Zhang, QZ (reprint author), Jinan Univ, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Inst Hydrobiol,Minist Educ, Guangzhou 510632, Guangdong, Peoples R China. EM zhangqzdr@126.com FU National High Technology Research and Development Program of China (863 Program) [2011AA10A216]; Marine and Fishery Special Project of Science and Technology in Guangdong Province [A201301B05, A201501B09]; Guangzhou Science and Technology Project [2013-J4100047]; Fundamental Research Funds for the Central Universities [21612111, 21613105] FX This work was supported by the National High Technology Research and Development Program of China (863 Program) (No. 2011AA10A216), the Marine and Fishery Special Project of Science and Technology in Guangdong Province (A201301B05, A201501B09), Guangzhou Science and Technology Project (No. 2013-J4100047), and Fundamental Research Funds for the Central Universities (21612111, 21613105). Usage of grass carp was approved by the Animal Experimentation Ethics Committee at Jinan University in Guangzhou, China. NR 33 TC 1 Z9 1 U1 3 U2 10 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD NOV 17 PY 2015 VL 117 IS 1 BP 13 EP 20 DI 10.3354/dao02925 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA CX1QC UT WOS:000365469700002 PM 26575152 ER PT J AU McGrath, JM Betzelberger, AM Wang, SW Shook, E Zhu, XG Long, SP Ainsworth, EA AF McGrath, Justin M. Betzelberger, Amy M. Wang, Shaowen Shook, Eric Zhu, Xin-Guang Long, Stephen P. Ainsworth, Elizabeth A. TI An analysis of ozone damage to historical maize and soybean yields in the United States SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE ozone; soybean; maize; air pollution; agriculture ID TROPOSPHERIC OZONE; CLIMATE-CHANGE; ATMOSPHERIC CO2; AIR-POLLUTION; CROP YIELDS; CULTIVARS; PLANT; O-3; PHOTOSYNTHESIS; AGRICULTURE AB Numerous controlled experiments find that elevated ground-level ozone concentrations ([O-3]) damage crops and reduce yield. There have been no estimates of the actual yield losses in the field in the United States from [O-3], even though such estimates would be valuable for projections of future food production and for cost-benefit analyses of reducing ground-level [O-3]. Regression analysis of historical yield, climate, and [O-3] data for the United States were used to determine the loss of production due to O-3 formaize (Zeamays) and soybean (Glycine max) from 1980 to 2011, showing that over that period production of rain-fed fields of soybean and maize were reduced by roughly 5% and 10%, respectively, costing approximately $9 billion annually. Maize, thought to be inherently resistant to O-3, was at least as sensitive as soybean to O-3 damage. Overcoming this yield loss with improved emission controls or more tolerant germplasm could substantially increase world food and feed supply at a time when a global yield jump is urgently needed. C1 [McGrath, Justin M.; Zhu, Xin-Guang; Long, Stephen P.; Ainsworth, Elizabeth A.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Betzelberger, Amy M.] Univ Cape Town, Dept Biol Sci, ZA-7701 Rondebosch, South Africa. [Wang, Shaowen] Univ Illinois, Dept Geog & Geog Informat Sci, Urbana, IL 61801 USA. [Wang, Shaowen] Univ Illinois, Natl Ctr Supercomp Applicat, Urbana, IL 61801 USA. [Shook, Eric] Kent State Univ, Dept Geog, Kent, OH 44242 USA. [Zhu, Xin-Guang] Chinese Acad Sci, Partner Inst Computat Biol, Max Planck Soc, Shanghai 200031, Peoples R China. [Long, Stephen P.; Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Long, Stephen P.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Ainsworth, Elizabeth A.] ARS, Global Change & Photosynthesis Res Unit, USDA, Urbana, IL 61801 USA. RP Long, SP (reprint author), Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. EM slong@illinois.edu; lisa.ainsworth@ars.usda.gov OI Long, Stephen/0000-0002-8501-7164 FU National Science Foundation [PGR-1238030, 0846655, 1047916] FX We thank Jesse McGrath and Nick Mangus for discussions about O3 regulations and help acquiring [O3] data from the Environmental Protection Agency. This work was supported in part by the National Science Foundation Grants PGR-1238030 (to E.A.A.) and 0846655 and 1047916 (to S.W.). NR 38 TC 12 Z9 12 U1 14 U2 39 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 17 PY 2015 VL 112 IS 46 BP 14390 EP 14395 DI 10.1073/pnas.1509777112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW7IA UT WOS:000365170400077 PM 26578785 ER PT J AU Lawrence, GB Hazlett, PW Fernandez, IJ Ouimet, R Bailey, SW Shortle, WC Smith, KT Antidormi, MR AF Lawrence, Gregory B. Hazlett, Paul W. Fernandez, Ivan J. Ouimet, Rock Bailey, Scott W. Shortle, Walter C. Smith, Kevin T. Antidormi, Michael R. TI Declining Acidic Deposition Begins Reversal of Forest-Soil Acidification in the Northeastern US and Eastern Canada SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SUGAR MAPLE TREES; UNITED-STATES; RED SPRUCE; NORTH-AMERICA; NEW-YORK; ADIRONDACK MOUNTAINS; CALCIUM ADDITION; CRITICAL LOADS; MINERAL SOIL; BASE CATIONS AB Decreasing trends in acidic deposition levels over the past several decades have led to partial chemical recovery of surface waters. However, depletion of soil Ca from acidic deposition has slowed surface water recovery and led to the impairment of both aquatic and terrestrial ecosystems. Nevertheless, documentation of acidic deposition effects on soils has been limited, and little is known regarding soil responses to ongoing acidic deposition decreases. In this study, resampling of soils in eastern Canada and the northeastern U.S. was done at 27 sites exposed to reductions in wet SO42- deposition of 5.7-76%, over intervals of 8-24 y. Decreases of exchangeable Al in the O horizon and increases in pH in the O and B horizons were seen at most sites. Among all sites, reductions in SO42- deposition were positively correlated with ratios (final sampling/initial sampling) of base saturation (P < 0.01) and negatively correlated with exchangeable Al ratios (P < 0.05) in the 0 horizon. However, base saturation in the B horizon decreased at one-third of the sites, with no increases. These results are unique in showing that the effects of acidic deposition on North American soils have begun to reverse. C1 [Lawrence, Gregory B.; Antidormi, Michael R.] US Geol Survey, New York Water Sci Ctr, Troy, NY 12180 USA. [Hazlett, Paul W.] Nat Resources Canada Canadian Forest Serv, Sault Ste Marie, ON P6A 2E5, Canada. [Fernandez, Ivan J.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Ouimet, Rock] Minist Foret Faune & Parcs Quebec, Direct Rech Forestiere, Quebec City, PQ G1P 3W8, Canada. [Bailey, Scott W.] US Forest Serv, USDA, Hubbard Brook Expt Forest, No Res Stn, Woodstock, NH 03262 USA. [Shortle, Walter C.; Smith, Kevin T.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Lawrence, GB (reprint author), US Geol Survey, New York Water Sci Ctr, 425 Jordan Rd, Troy, NY 12180 USA. EM glawrenc@usgs.gov OI Bailey, Scott/0000-0002-9160-156X FU Northeastern States Research Cooperative [110148]; New York Energy and Research Authority [25522]; Forest Ecosystems Integrity Project of Natural Resources Canada-Canadian Forest Service; U.S. Geological Survey FX Funding was provided by the Northeastern States Research Cooperative (no. 110148), the New York Energy and Research Authority (no. 25522), the Forest Ecosystems Integrity Project of Natural Resources Canada-Canadian Forest Service, and the U.S. Geological Survey. We thank Mark David, University of Illinois, for providing archived soil samples, James Shanley, U.S. Geological Survey, for facilitating sampling at Sleepers River, VT, and Michael McHale for a helpful manuscript review. We also thank the Equinox Preservation Trust for permission to sample. The Hubbard Brook Experimental Forest is maintained and operated by the U.S. Forest Service, Northern Research Station, Newtown Square, PA. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 56 TC 11 Z9 11 U1 6 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD NOV 17 PY 2015 VL 49 IS 22 BP 13103 EP 13111 DI 10.1021/acs.est.5b02904 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CW7AV UT WOS:000365151200003 PM 26495963 ER PT J AU Yi, P Pignatello, JJ Uchimiya, M White, JC AF Yi, Peng Pignatello, Joseph J. Uchimiya, Minori White, Jason C. TI Heteroaggregation of Cerium Oxide Nanoparticles and Nanoparticles of Pyrolyzed Biomass SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID MULTIWALLED CARBON NANOTUBES; FULLERENE C-60 NANOPARTICLES; CHARGED COLLOIDAL PARTICLES; CEO2 NANOPARTICLES; HEMATITE NANOPARTICLES; ENGINEERED NANOPARTICLES; AGGREGATION KINETICS; AQUEOUS ENVIRONMENTS; SOLUTION CHEMISTRY; NATURAL COLLOIDS AB Heteroaggregation with indigenous particles is critical to the environmental mobility of engineered nanomaterials (ENM). We studied heteroaggregation of ceria nanoparticles (n-CeO2), as a model for metal oxide ENM, with nanoparticles of pyrogenic carbonaceous material (n-PCM) derived from pecan shell biochar, a model for natural chars and human-made chars used in soil remediation and agriculture. The TEM and STEM images of n-PCM identify both hard and soft particles, both C-rich and C,O,Ca-containing particles (with CaCO3 crystals), both amorphous and onion-skin C-rich particles, and traces of nanotubes. Heteroaggregation was evaluated at constant n-CeO2, variable n-PCM concentration by monitoring hydrodynamic diameter by dynamic light scattering and zeta-potential under conditions where n-PCM is invisible. At pH 5.3, where n-CeO2 and n-PCM are positively and negatively charged, respectively, and each stable to homoaggregation, heteroaggregation is favorable and occurs by a charge neutralizationcharge reversal mechanism (CNCR): in this mechanism, primary heteroaggregates that form in the initial stage are stable at low or high n-PCM concentration due to electrostatic repulsion, but unstable at intermediate n-PCM concentration, leading to secondary heteroaggregation. The greatest instability coincides with full charge neutralization. At pH 7.1, where n-CeO2 is neutral and unstable alone, and n-PCM is negative and stable alone, heteroaggregation occurs by a charge-accumulation, coreshell stabilization (CACS) mechanism: n-PCM binds to and forms a negatively charged shell on the neutral surface of the nascent n-CeO2 core, stabilizing the coreshell heteraggregate at a size that decreases with n-PCM concentration. The CNCR and CACS mechanisms give fundamental insight into heteroaggregation between oppositely charged, and between neutral and charged nanoparticles. C1 [Yi, Peng; Pignatello, Joseph J.] Connecticut Agr Expt Stn, Dept Environm Sci, New Haven, CT 06511 USA. [Yi, Peng] Florida Atlantic Univ, Dept Civil Environm & Geomat Engn, Boca Raton, FL 33431 USA. [Uchimiya, Minori] USDA ARS, New Orleans, LA 70124 USA. [White, Jason C.] Connecticut Agr Expt Stn, Dept Analyt Chem, New Haven, CT 06511 USA. RP Pignatello, JJ (reprint author), Connecticut Agr Expt Stn, Dept Environm Sci, New Haven, CT 06511 USA. EM joseph.pignatello@ct.gov FU U.S. Department of Agriculture NIFA AFRI, Nanotechnology for Agricultural and Food Systems Program [2012-67021-19300] FX This research was funded by a grant (2012-67021-19300) from the U.S. Department of Agriculture NIFA AFRI, Nanotechnology for Agricultural and Food Systems Program. We thank Michael Rooks, Yale Institute for Nanoscience and Quantum Engineering for assistance in TEM imaging and J. Michael McCaffery, Integrated Imaging Center, Johns Hopkins University for performing the cryo-TEM imaging. NR 61 TC 2 Z9 2 U1 16 U2 62 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD NOV 17 PY 2015 VL 49 IS 22 BP 13294 EP 13303 DI 10.1021/acs.est.5b03541 PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CW7AV UT WOS:000365151200024 PM 26461459 ER PT J AU Xu, Y Shen, ZH Li, DX Guo, QF AF Xu, Yue Shen, Zehao Li, Daoxin Guo, Qinfeng TI Pre-Dispersal Seed Predation in a Species-Rich Forest Community: Patterns and the Interplay with Determinants SO PLOS ONE LA English DT Article ID RAIN-FOREST; 3 GORGES; PREDISPERSAL; SIZE; PLANT; CONSEQUENCES; INSECTS; MODELS; REPRODUCTION; GERMINATION AB Pre-dispersal seed predation (PDSP) is commonly observed in woody plants, and recognized as a driver of seed production variability that is critical for successful regeneration. Earlier studies on PDSP and its determinants were mostly species specific, with community- level PDSP rarely estimated; and the interactions between the temporal variability of seed production and PDSP remain elusive. In this study, the community seed rain of woody plants in a mixed evergreen-deciduous broadleaf forest was monitored for seven years. We examined predation on collected seeds and analyzed the determinants of PDSP. PDSP was recorded in 17 out of 44 woody plant species, and three-quarters of PDSP was due to insect predators. Annual seed production varied substantially at community level, reversely linked with the temporal variation of PDSP rate. The PDSP rate was biased regarding fruit types, and being significantly correlated with seed mass when using phylogenetic independent contrasts (PICs) or without taking into account phylogenetic relations, especially for nuts. PDSP rate was also negatively correlated with seed density, showing a threshold-related predator satiation effect. The community-level PDSP rate was primarily determined by tree height, fruit type, and interannual variation of seed production and seed mass. Our analysis revealed a causal link between seed production and the dynamics of PDSP rate at the community level. The predator satiation effect was primarily contributed by the dominant species, whereas the rare species seemed to apply a distinct "hide-and-seek" strategy to control the risk of PDSP. The mechanistic difference of seed production between the common and rare species can shed new light on species coexistence and community assembly. Long-term monitoring of both seed rain and seed predation is required for understanding the ecological and evolutionary implications of species regeneration strategies in a species-rich forest community. C1 [Xu, Yue; Shen, Zehao] Peking Univ, Coll Urban & Environm Sci, Minist Educ, Key Lab Earth Surface Proc, Beijing 100871, Peoples R China. [Li, Daoxin] Dalaoling Natl Forest Pk, Yichang, Hubei, Peoples R China. [Guo, Qinfeng] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Res Triangle Pk, NC USA. RP Shen, ZH (reprint author), Peking Univ, Coll Urban & Environm Sci, Minist Educ, Key Lab Earth Surface Proc, Beijing 100871, Peoples R China. EM shenzh@urban.pku.edu.cn FU National Natural Science Foundation of China [31170449, 30770386, 31321061] FX This study was supported by grants from the National Natural Science Foundation of China (http://www.nsfc.gov.cn/, Grant numbers: 31170449, 30770386 and 31321061). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 62 TC 0 Z9 0 U1 3 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 17 PY 2015 VL 10 IS 11 AR e0143040 DI 10.1371/journal.pone.0143040 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW7BR UT WOS:000365153400031 PM 26575270 ER PT J AU Burdett, CL Kraus, BR Garza, SJ Miller, RS Bjork, KE AF Burdett, Christopher L. Kraus, Brian R. Garza, Sarah J. Miller, Ryan S. Bjork, Kathe E. TI Simulating the Distribution of Individual Livestock Farms and Their Populations in the United States: An Example Using Domestic Swine (Sus scrofa domesticus) Farms SO PLOS ONE LA English DT Article ID EVOLUTIONARY; DATABASE; AREAS; HOG AB Livestock distribution in the United States (U.S.) can only be mapped at a county-level or worse resolution. We developed a spatial microsimulation model called the Farm Location and Agricultural Production Simulator (FLAPS) that simulated the distribution and populations of individual livestock farms throughout the conterminous U.S. Using domestic pigs (Sus scrofa domesticus) as an example species, we customized iterative proportional-fitting algorithms for the hierarchical structure of the U.S. Census of Agriculture and imputed unpublished state-or county-level livestock population totals that were redacted to ensure confidentiality. We used a weighted sampling design to collect data on the presence and absence of farms and used them to develop a national-scale distribution model that predicted the distribution of individual farms at a 100 m resolution. We implemented microsimulation algorithms that simulated the populations and locations of individual farms using output from our imputed Census of Agriculture dataset and distribution model. Approximately 19% of county-level pig population totals were unpublished in the 2012 Census of Agriculture and needed to be imputed. Using aerial photography, we confirmed the presence or absence of livestock farms at 10,238 locations and found livestock farms were correlated with open areas, cropland, and roads, and also areas with cooler temperatures and gentler topography. The distribution of swine farms was highly variable, but cross-validation of our distribution model produced an area under the receiver-operating characteristics curve value of 0.78, which indicated good predictive performance. Verification analyses showed FLAPS accurately imputed and simulated Census of Agriculture data based on absolute percent difference values of <0.01% at the state-to-national scale, 3.26% for the county-to-state scale, and 0.03% for the individual farm-to-county scale. Our output data have many applications for risk management of agricultural systems including epidemiological studies, food safety, biosecurity issues, emergency-response planning, and conflicts between livestock and other natural resources. C1 [Burdett, Christopher L.; Kraus, Brian R.; Garza, Sarah J.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Miller, Ryan S.; Bjork, Kathe E.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ctr Anim Hlth, Ft Collins, CO USA. [Miller, Ryan S.; Bjork, Kathe E.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ctr Epidemiol, Ft Collins, CO USA. RP Burdett, CL (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. EM Chris.Burdett@colostate.edu OI Miller, Ryan/0000-0003-3892-0251 FU U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Center for Epidemiology and Animal Health; U.S. Department of Health and Human Services, Food and Drug Administration, Center for Food Safety and Applied Nutrition; USDA [11-9208-0301, 13-9208-0336] FX This work was funded by the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Center for Epidemiology and Animal Health (http://www.aphis.usda.gov/wps/portal/?1dmy&urile=wcm:path:/APHIS_Conten t_Library/SA_Our_Focus/SA_Animal_Health/SA_Program_Overview/SA_CEAH). Additional funding was provided by the U.S. Department of Health and Human Services, Food and Drug Administration, Center for Food Safety and Applied Nutrition (http://www.fda.gov/AboutFDA/CentersOffices/OfficeofFoods/CFSAN/) through an interagency grant to APHIS-VS-CEAH. Grant numbers supporting this work include USDA grant numbers 11-9208-0301 (interagency grant from FDA to USDA represented here) and 13-9208-0336. These grants were received by CB, who served as principle investigator at Colorado State University. The co-authors at APHIS-VS-CEAH had subordinate roles in study design, data analysis, publication and manuscript preparation. NR 44 TC 1 Z9 1 U1 6 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 16 PY 2015 VL 10 IS 11 AR e0140338 DI 10.1371/journal.pone.0140338 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW5XP UT WOS:000365070700002 PM 26571497 ER PT J AU Nguyen, P Greene, E Ishola, P Huff, G Donoghue, A Bottje, W Dridi, S AF Phuong Nguyen Greene, Elizabeth Ishola, Peter Huff, Geraldine Donoghue, Annie Bottje, Walter Dridi, Sami TI Chronic Mild Cold Conditioning Modulates the Expression of Hypothalamic Neuropeptide and Intermediary Metabolic-Related Genes and Improves Growth Performances in Young Chicks SO PLOS ONE LA English DT Article ID REGULATES FOOD-INTAKE; MTOR COMPLEX 1; GALLUS-DOMESTICUS; SKELETAL-MUSCLE; EARLY-AGE; NONSHIVERING THERMOGENESIS; TRANSCRIPTION FACTOR; ANTIBODY-RESPONSES; IMMUNE-RESPONSES; MESSENGER-RNA AB Background Low environmental temperatures are among the most challenging stressors in poultry industries. Although landmark studies using acute severe cold exposure have been conducted, still the molecular mechanisms underlying cold-stress responses in birds are not completely defined. In the present study we determine the effect of chronic mild cold conditioning (CMCC) on growth performances and on the expression of key metabolic-related genes in three metabolically important tissues: brain (main site for feed intake control), liver (main site for lipogenesis) and muscle (main site for thermogenesis). Methods 80 one-day old male broiler chicks were divided into two weight-matched groups and maintained in two different temperature floor pen rooms (40 birds/room). The temperature of control room was 32 degrees C, while the cold room temperature started at 26.7 degrees C and gradually reduced every day (1 degrees C/day) to reach 19.7 degrees C at the seventh day of the experiment. At day 7, growth performances were recorded (from all birds) and blood samples and tissues were collected (n = 10). The rest of birds were maintained at the same standard environmental condition for two more weeks and growth performances were measured. Results Although feed intake remained unchanged, body weight gain was significantly increased in CMCC compared to the control chicks resulting in a significant low feed conversion ratio (FCR). Circulating cholesterol and creatine kinase levels were higher in CMCC chicks compared to the control group (P<0.05). CMCC significantly decreased the expression of both the hypothalamic orexigenic neuropeptide Y (NPY) and anorexigenic cocaine and amphetamine regulated transcript (CART) in chick brain which may explain the similar feed intake between the two groups. Compared to the control condition, CMCC increased the mRNA abundance of AMPK alpha 1/alpha 2 and decreased mTOR gene expression (P<0.05), the master energy and nutrient sensors, respectively. It also significantly decreased the expression of fatty acid synthase (FAS) gene in chick brain compared to the control. Although their roles are still unknown in avian species, adiponectin (Adpn) and its related receptors (Adi-poR1 and 2) were down regulated in the brain of CMCC compared to control chicks (P<0.05). In the liver, CMCC significantly down regulated the expression of lipogenic genes namely FAS, acetyl-CoA carboxylase alpha (ACC alpha) and malic enzyme (ME) and their related transcription factors sterol regulatory element binding protein 1/2 (SREBP-1 and 2). Hepatic mTOR mRNA levels and phosphorylated mTOR at Ser(2448) were down regulated (P<0.05), however phosphorylated ACC alpha(Ser79) (inactivation) was up regulated (P<0.05) in CMCC compared to control chicks, indicating that CMCC switch hepatic catabolism on and inhibits hepatic lipogenesis. In the muscle however, CMCC significantly up regulated the expression of carnitine palmitoyltransferase 1 (CPT-1) gene and the mRNA and phosphorylated protein levels of mTOR compared to the control chicks, indicating that CMCC enhanced muscle fatty acid beta-oxidation. Conclusions In conclusion, this is the first report indicating that CMCC may regulate AMPK-mTOR expression in a tissue specific manner and identifying AMPK-mTOR as a potential molecular signature that controls cellular fatty acid utilization (inhibition of hepatic lipogenesis and induction of muscle fatty acid beta-oxidation) to enhance growth performance during mild cold acclimation. C1 [Phuong Nguyen; Greene, Elizabeth; Ishola, Peter; Bottje, Walter; Dridi, Sami] Univ Arkansas, Ctr Excellence Poultry Sci, Fayetteville, AR 72701 USA. [Huff, Geraldine; Donoghue, Annie] ARS, USDA, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. RP Dridi, S (reprint author), Univ Arkansas, Ctr Excellence Poultry Sci, Fayetteville, AR 72701 USA. EM dridi@uark.edu FU Arkansas State FX This study was supported by Arkansas State start-up fundings. NR 73 TC 0 Z9 0 U1 4 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 16 PY 2015 VL 10 IS 11 AR e0142319 DI 10.1371/journal.pone.0142319 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CW5XP UT WOS:000365070700032 PM 26569484 ER PT J AU Norrbom, AL Rodriguez, EJ Steck, GJ Sutton, BA Nolazco, N AF Norrbom, Allen L. Rodriguez, Erick J. Steck, Gary J. Sutton, Bruce A. Nolazco, Norma TI New species and host plants of Anastrepha (Diptera: Tephritidae) primarily from Peru and Bolivia SO ZOOTAXA LA English DT Article DE fruit flies; taxonomy; host plants; Neotropical Region AB Twenty-eight new species of Anastrepha are described and illustrated: A. acca (Bolivia, Peru), A. adami (Peru), A. amplidentata (Bolivia, Peru), A. annonae (Peru), A. breviapex (Peru), A. caballeroi (Peru), A. camba (Bolivia, Peru), A. cicra( Bolivia, Peru), A. disjuncta (Peru), A. durantae (Peru), A. echaratiensis (Peru), A. eminens (Peru), A. ericki (Peru), A. gonzalezi (Bolivia, Peru), A. guevarai (Peru), A. gusi (Peru), A. kimi (Colombia, Peru), A. korytkowskii (Bolivia, Peru), A. latilanceola (Bolivia, Peru), A. melanoptera (Peru), A. mollyae (Bolivia, Peru), A. perezi (Peru), A. psidivora (Peru), A. robynae (Peru), A. rondoniensis (Brazil, Peru), A. tunariensis (Bolivia, Peru), A. villosa (Bolivia), and A. zacharyi (Peru). The following host plant records are reported: A. amplidentata from Spondias mombin L. (Anacardiaceae); A. caballeroi from Quararibea malacocalyx A. Robyns & S. Nilsson (Malvaceae); A. annonae from Annona mucosa Jacq. and Annonasp. (Annonaceae); A. durantae from Duranta peruviana Moldenke (Verbenaceae); and A. psidivora from Psidium guajavaL. (Myrtaceae). C1 [Norrbom, Allen L.] ARS, USDA, Smithsonian Inst, Systemat Entomol Lab, Washington, DC 20013 USA. [Rodriguez, Erick J.; Steck, Gary J.; Sutton, Bruce A.] Florida Dept Agr & Consumer Serv, Div Plant Ind Entomol, Gainesville, FL USA. [Nolazco, Norma] Ctr Diagnost Sanidad Vegetal, Serv Nacl Sanidad Agr, La Molina, Peru. RP Norrbom, AL (reprint author), ARS, USDA, Smithsonian Inst, Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA. EM allen.norrbom@ars.usda.gov FU USDA; APHIS; CPHST; IS; [3.0342]; [13-8131-0291-CA] FX The majority of specimens examined in this study were collected through the USDA Farm Bill project "Enhancement of Fruit Fly Larvae Identification and Taxonomy" (Project Coordinator G.J. Steck, ADODR Norman Barr; project numbers 3.0342 (2012) and 13-8131-0291-CA (2013)). All of the authors contributed to the planning of the project and the collection, rearing, and sorting of the samples; the senior author was responsible for the taxonomic decisions and descriptions. We are grateful to Megan MacDowell, Valerie Peterson and Eileen Rosin of ACA, Laura Samaniego, Patrick Campbell, Adrian Tejedor, Yngrid Espinoza, Fernando Araoz, Norma Fernandez and Oswaldo Coycosi of ACCA, Erick Yabar of UNSAAC, and Erika Paliza and Frank Azorsa of CEBIO, whose collaboration was essential to the success of the project. We also sincerely thank the volunteers, students, and contractors who serviced our traps, particularly Tim Perez, Jorge Caballero, Tito Guevara, Marlene "Charito" Choque, Timo Forster, Jeniffer Alvarez Baca, Jonathan Leon Flores, Hugo Roger Ninantay Rivera, Wendy Coaquira, and Nicolas Castro. The following curators and institutions also kindly loaned or provided specimens for study: Jere Schweikert and Paul H. Arnaud, Jr. (CAS), Juan Jose Lagrava Sanchez and Elizabeth Quisberth (DSASC), Emilio Arevalo Penaranda (ICAB), Manuel Zumbado (INBio), Luis Joly (IZAM), Gerardo Lamas (MHNJP), Julieta Ledesma (MNKM), Freddy Colque (SENASAG), Uwe Kallweit (SMT), and J. Ziegler (ZMHU). William Farfan (Wake Forest University) and Pedro Acevedo and Mark Strong (Smithsonian Institution) kindly identified the host plants. Lucrecia Rodriguez, Taina Litwak, Elizabeth Roberts, George Venable, and Jerrett McCormick helped to produce many of the illustrations, and Terrence Walters (USDA, APHIS, CPHST) secured funding for some of the illustration work. Annabella Reszczynski (USDA, APHIS, IS) helped obtain funding for ALN to travel to Peru which facilitated collaboration with SENASA, and George "Andy" Ball and Cesar Orozco (USDA, APHIS, IS) assisted in multiple ways with travel and sampling efforts in Peru and Bolivia. We also thank Roberto Zucchi and Norman Woodley for their comments on previous drafts of the manuscript. USDA is an equal opportunity provider and employer. NR 16 TC 2 Z9 2 U1 1 U2 8 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD NOV 16 PY 2015 VL 4041 IS 1 BP 1 EP + PG 93 WC Zoology SC Zoology GA CW6WT UT WOS:000365139800001 PM 26624697 ER PT J AU Siddappaji, MH Scholes, DR Krishnankutty, SM Calla, B Clough, SJ Zielinski, RE Paige, KN AF Siddappaji, Madhura H. Scholes, Daniel R. Krishnankutty, Sindhu M. Calla, Bernarda Clough, Steven J. Zielinski, Raymond E. Paige, Ken N. TI The role of invertases in plant compensatory responses to simulated herbivory SO BMC PLANT BIOLOGY LA English DT Article DE Arabidopsis; Invertase; Overcompensation; Herbivory; G6PD1; Sucrose; Oxidative pentose phosphate pathway ID IPOMOPSIS-AGGREGATA; CYTOSOLIC INVERTASE; ARABIDOPSIS-THALIANA; CELL-DEVELOPMENT; BENEFIT PLANTS; SCARLET-GILIA; NORMAL GROWTH; TOLERANCE; OVERCOMPENSATION; PLASTICITY AB Background: The ability of a plant to overcome animal-induced damage is referred to as compensation or tolerance and ranges from undercompensation (decreased fitness when damaged) to overcompensation (increased fitness when damaged). Although it is clear that genetic variation for compensation exists among plants, little is known about the specific genetic underpinnings leading to enhanced fitness. Our previous study identified the enzyme GLUCOSE-6-PHOSPHATE DEHYDROGENASE 1 (G6PD1) as a key regulator contributing to the phenomenon of overcompensation via its role in the oxidative pentose phosphate pathway (OPPP). Apart from G6PD1 we also identified an invertase gene which was up-regulated following damage and that potentially integrates with the OPPP. The invertase family of enzymes hydrolyze sucrose to glucose and fructose, whereby the glucose produced is shunted into the OPPP and presumably supports plant regrowth, development, and ultimately compensation. In the current study, we measured the relative expression of 12 invertase genes over the course of plant development in the Arabidopsis thaliana genotypes Columbia-4 and Landsberg erecta, which typically overcompensate and undercompensate, respectively, when damaged. We also compared the compensatory performances of a set of invertase knockout mutants to the Columbia-4 wild type. Results: We report that Columbia-4 significantly up-regulated 9 of 12 invertase genes when damaged relative to when undamaged, and ultimately overcompensated for fruit production. Landsberg erecta, in contrast, down-regulated two invertase genes following damage and suffered reduced fitness. Knockout mutants of two invertase genes both exhibited significant undercompensation for fruit production, exhibiting a complete reversal of the wild type Col-4's overcompensation. Conclusion: Collectively, these results confirm that invertases are essential for not only normal plant growth and development, but also plants' abilities to regrow and ultimately compensate for fitness following apical damage. C1 [Siddappaji, Madhura H.; Scholes, Daniel R.; Paige, Ken N.] Univ Illinois, Dept Anim Biol, Urbana, IL 61801 USA. [Scholes, Daniel R.] Univ Indianapolis, Dept Biol, Indianapolis, IN 46227 USA. [Krishnankutty, Sindhu M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Calla, Bernarda] USDA ARS, Pacific Basin, Agr Res Ctr, Hilo, HI 96720 USA. [Clough, Steven J.] USDA ARS, Urbana, IL 61801 USA. [Clough, Steven J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Zielinski, Raymond E.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. RP Paige, KN (reprint author), Univ Illinois, Dept Anim Biol, Urbana, IL 61801 USA. EM k-paige@illinois.edu FU National Science Foundation [DEB 1010868, DEB-1146085] FX This study was funded in part by grants from the National Science Foundation (DEB 1010868 and DEB-1146085) to KNP. Thanks to Dr. Thomas Newman for assistance in performing gene expression analyses. NR 44 TC 0 Z9 0 U1 5 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD NOV 16 PY 2015 VL 15 AR 278 DI 10.1186/s12870-015-0655-6 PG 12 WC Plant Sciences SC Plant Sciences GA CV9SS UT WOS:000364627500001 PM 26572986 ER PT J AU Wang, AJ Yang, ZH Grinchuk, V Smith, A Qin, BL Lu, NH Wang, D Wang, HB Ramalingam, TR Wynn, TA Urban, JF Shea-Donohue, T Zhao, AP AF Wang, An-Jiang Yang, Zhonghan Grinchuk, Viktoriya Smith, Allen Qin, Bolin Lu, Nonghua Wang, Duan Wang, Hongbing Ramalingam, Thirumalai R. Wynn, Thomas A. Urban, Joseph F., Jr. Shea-Donohue, Terez Zhao, Aiping TI IL-25 or IL-17E Protects against High-Fat Diet-Induced Hepatic Steatosis in Mice Dependent upon IL-13 Activation of STAT6 SO JOURNAL OF IMMUNOLOGY LA English DT Article ID LIVER-DISEASE; TYPE-2 IMMUNITY; KUPFFER CELLS; ALTERNATIVE ACTIVATION; NEMATODE INFECTION; INTERLEUKIN-25; INFLAMMATION; MACROPHAGES; RECEPTOR; PROGRESSION AB IL-25 or IL-17E is a member of IL-17 cytokine family and has immune-modulating activities. The role of IL-25 in maintaining lipid metabolic homeostasis remains unknown. We investigated the effects of exogenous IL-25 or deficiency of IL-25 on hepatic lipid accumulation. IL-25 expression was examined in paraffin-embedded tissue sections of liver from patients or in the livers from mice. Mouse model of steatosis was induced by feeding a high-fat diet (HFD). Extent of steatosis as well as expression of cytokines, key enzymes for lipid metabolic pathways, markers for Kupffer cells/macrophages, and lipid droplet (LD) proteins, were analyzed. Our results show that hepatic steatosis in mice was accompanied by increased LD proteins, but decreased IL-25 in the liver. Decreased hepatic IL-25 was also observed in patients with fatty liver. Administration of IL-25 to HFD-fed wild-type mice led to a significant improvement in hepatic steatosis. This effect was associated with increased expression of IL-13, development of alternatively activated Kupffer cells/macrophages, and decreased expression of LD proteins in the liver. In contrast, administration of IL-25 to HFD-fed mice deficient in STAT6 or IL-13 had no effects. In addition, stimulation of primary hepatocytes with IL-13, but not IL-25, resulted in downregulation of LD proteins. Finally, mice deficient in IL-25 had exacerbated hepatic lipid accumulation when fed the HFD. These data demonstrate that dysregulated IL-25 expression contributes to lipid accumulation, whereas exogenous IL-25 protects against hepatic steatosis through IL-13 activation of STAT6. IL-25 and IL-13 are potential therapeutic agents for hepatic steatosis and associated pathologies. C1 [Wang, An-Jiang; Yang, Zhonghan; Grinchuk, Viktoriya; Qin, Bolin; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Dept Radiat Oncol, Baltimore, MD 21201 USA. [Wang, An-Jiang; Yang, Zhonghan; Grinchuk, Viktoriya; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Wang, An-Jiang; Lu, Nonghua] Nanchang Univ, Affiliated Hosp 1, Dept Gastroenterol & Hepatol, Nanchang 330006, Peoples R China. [Yang, Zhonghan] Sun Yat Sen Univ, Zhongshan Med Sch, Dept Biochem, Guangzhou 510080, Guangdong, Peoples R China. [Smith, Allen; Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Wang, Duan; Wang, Hongbing] Univ Maryland, Sch Med, Dept Pharmaceut Sci, Baltimore, MD 21201 USA. [Ramalingam, Thirumalai R.; Wynn, Thomas A.] NIAID, Div Parasitol, NIH, Bethesda, MD 20892 USA. RP Zhao, AP (reprint author), NIH, Div Physiol & Pathol Sci, Ctr Sci Review, 6701 Rockledge Dr, Bethesda, MD 20892 USA. EM aiping.zhao@nih.gov FU National Institutes of Health [R01-DK083418, R01-AI/DK49316, R01-DK061652]; U.S. Department of Agriculture Current Research Information System project [8040-51000-058-00]; National Natural Science Fund of China [81460122] FX This work was supported by National Institutes of Health Grants R01-DK083418 (to A.Z.), R01-AI/DK49316 (to T.S.-D.), and R01-DK061652 (to H.W.), U.S. Department of Agriculture Current Research Information System project 8040-51000-058-00 (to A.S. and J.F.U.), and National Natural Science Fund of China Grant 81460122 (to A.-J.W.). NR 43 TC 3 Z9 3 U1 1 U2 4 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD NOV 15 PY 2015 VL 195 IS 10 BP 4771 EP 4780 DI 10.4049/jimmunol.1500337 PG 10 WC Immunology SC Immunology GA CW2LC UT WOS:000364822800027 PM 26423151 ER PT J AU Flerchinger, GN Reba, ML Link, TE Marks, D AF Flerchinger, Gerald N. Reba, Michele L. Link, Timothy E. Marks, Danny TI Modeling temperature and humidity profiles within forest canopies SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Multi-layer canopy model; SHAW model; Forest canopy; Micrometeorology ID SURFACE-ENERGY FLUXES; SIMULTANEOUS HEAT; WATER-BALANCE; SHAW MODEL; PLANT CANOPIES; VEGETATION; CARBON; EVAPOTRANSPIRATION; PARAMETERIZATION; DISTRIBUTIONS AB Physically-based models are a powerful tool to help understand interactions of vegetation, atmospheric dynamics, and hydrology, and to test hypotheses regarding the effects of land cover, management, hydrometeorology, and climate variability on ecosystem processes. The purpose of this paper is to evaluate recent modifications and further refinements to a multi-layer plant canopy model for simulating temperature and water vapor within three diverse forest canopies: a 4.5-m tall aspen thicket, a 15-m tall aspen canopy, and a 60-m tall Douglas fir canopy. Performance of the model was strongly related to source strength and profile stability within the canopy. Root mean square deviation (RMSD) between simulated and observed values tended to be higher for the summer periods when there was much more heat and vapor added to the canopy space due to solar warming and transpiration. Conversely, RMSD for vapor pressure was lowest for the winter periods when vapor additions within the canopy space were minimal. RMSD for temperature ranged from 0.1 degrees C for the top of the 15-m aspen canopy during the winter to 1.6 degrees C for the bottom of the 4.5-m aspen thicket during the summer period. RMSD for vapor pressure ranged from 0.002 kPa for the top of the 15-m aspen canopy during winter to 0.141 kPa for the bottom of the 4.5-m aspen thicket during the summer. Unstable profile conditions were simulated better by the model than stable conditions for all sites. RMSD for temperature at the bottom of the 4.5-m aspen, 15-m aspen and 60-m Douglas fir were 0.89, 0.77, and 0.85 degrees C, respectively, for unstable conditions compared to 1.44, 0.89 and 1.16 degrees C for stable conditions. Stable profiles are more challenging to accurately simulate because dispersion within a stable profile is lower thereby creating larger gradients. Temperature differences between the bottom and above canopy sensors were within 3 degrees C for unstable conditions for all sites, but were as much as -10 degrees C under stable conditions. The model exhibited the greatest discrepancies relative to measurements in the 4.5-m aspen thicket under stable conditions, likely due to horizontal ejections from this relatively small patch of vegetation that could not be addressed by the one-dimensional model. At each site, the model performed best near the top of canopy where the air was well mixed and gradients between it the meteorological conditions above the canopy used to force the model were minimal. Published by Elsevier B.V. C1 [Flerchinger, Gerald N.; Marks, Danny] ARS, USDA, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Reba, Michele L.] ARS, USDA, Natl Sedimentat Lab, Jonesboro, AR USA. [Link, Timothy E.] Univ Idaho, Coll Nat Resources, Moscow, ID 83843 USA. RP Flerchinger, GN (reprint author), ARS, USDA, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. EM gerald.flerchinger@ars.usda.gov FU National Science Foundation (CBET Award) [0854553]; USGS Northwest Climate Science Center; Western Regional Center (WESTGEC) of the National Institute for Global Environmental Change (NIGEC) [DE-FC03-90ER61010]; US Forest Service (USFS) FX USDA is an equal opportunity provider and employer. Support for portions of this research was provided by the National Science Foundation (CBET Award 0854553), the USGS Northwest Climate Science Center, the Western Regional Center (WESTGEC) of the National Institute for Global Environmental Change (NIGEC) under Cooperative Agreement No. DE-FC03-90ER61010, and the US Forest Service (USFS). NR 42 TC 3 Z9 4 U1 11 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD NOV 15 PY 2015 VL 213 BP 251 EP 262 DI 10.1016/j.agrformet.2015.07.007 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CR3TG UT WOS:000361255400023 ER PT J AU Fanta, GF Kenar, JA Felker, FC AF Fanta, George F. Kenar, James A. Felker, Frederick C. TI Nanoparticle formation from amylose-fatty acid inclusion complexes prepared by steam jet cooking SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Starch; Amylose; Oleic acid; Spherulite; Inclusion complex; Steam jet cooking; Nanoparticles ID WAXY MAIZE STARCH; NANOCRYSTALS; NANOPRECIPITATION; HYDROLYSIS; ULTRASONICATION; NANOSPHERES; BEHAVIOR; WATER AB Starch-based nanoparticles are of increasing interest for use as biobased fillers in composites with rubber and other polymers. Different methods have been reported for producing them, many requiring lengthy or complicated procedures. The purpose of this study was to determine whether the previously reported formation of spherulites by slowly cooling jet cooked dispersions of amylose inclusion complexes could be modified for nanoparticle synthesis. High-amylose cornstarch combined with oleic acid was jet cooked and then cooled at different rates ranging from 110 mm to 10 s. Dynamic light scattering and SEM analysis showed that nanoparticles with diameters from 63 to 375 nm were obtained. X-ray diffraction analysis confirmed that they were comprised of V-6 amylose complexes. Cooling rate and starch concentration affected yield of nanoparticles and their tendency to aggregate. Large quantities of starch-based nanoparticles can be prepared using this scalable method for further characterization and application development. Published by Elsevier B.V. C1 [Fanta, George F.] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Kenar, James A.; Felker, Frederick C.] ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Felker, FC (reprint author), ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM george.fanta@ars.usda.gov; jim.kenar@ars.usda.gov; frederick.felker@ars.usda.gov NR 36 TC 1 Z9 1 U1 5 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 15 PY 2015 VL 74 BP 36 EP 44 DI 10.1016/j.indcrop.2015.04.046 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA CQ9QD UT WOS:000360948900006 ER PT J AU Cermak, SC Bredsguard, JW Roth, KL Thompson, T Feken, KA Isbell, TA Murray, RE AF Cermak, Steven C. Bredsguard, Jakob W. Roth, Katie L. Thompson, Travis Feken, Kati A. Isbell, Terry A. Murray, Rex E. TI Synthesis and physical properties of new coco-oleic estolide branched esters SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biodegradable oils; Esters; Estolides; Physical properties; Pour points; Viscosities ID OXIDATIVE STABILITY; 2-ETHYLHEXYL ESTERS; FATTY-ACIDS; LESQUERELLA; DERIVATIVES; CASTOR AB Oils derived from vegetable oils tend to not meet the standards for industrial lubricants because of unacceptable low temperature properties, pour point (PP) and/or cloud point (CP). However, a catalytic amount of perchloric acid with oleic and coconut (coco) fatty acids produced a coco-oleic estolide. The resulting coco-oleic estolide was separated into two components based on the extent of oligomerization: coco-oleic dimer estolide and coco-oleic trimer plus estolide. These two estolides were then esterified with a series of different branched alcohols; the coco-oleic dimer estolide esters had the lowest PP = -45 degrees C when with esterified 2-hexyldecanol and PP = -39 degrees C with 2-octyldodecanol. The best CP performer from the same series was the 2-octyldodecanol ester, CP = -37 degrees C. The coco-oleic trimer plus estolide esters had slightly higher PPs (-24 to -39 degrees C) with the same alcohols. The viscosities and viscosity indexes were as expected in terms of trends. The coco-oleic dimer estolide esters ranged 27.5-51.7 cSt @ 40 degrees C and 3.0-9.5 cSt @ 100 degrees C, whereas the coco-oleic trimer plus estolide esters ranged 120.8-227.7 cSt @ 40 degrees C and 17.9-29.4 cSt at 100 degrees C for the same series as the dimer esters. Outside the series tested, an iso-stearyl trimer plus ester had the highest reported viscosity of 417.3 cSt @40 degrees C and 38.9 cSt @ 100 degrees C. Because these new branched estolide esters have excellent viscosity and low temperature physical properties without the addition of other chemicals, they minimize the effect on the environment while replacing nonrenewable products. Published by Elsevier B.V. C1 [Cermak, Steven C.; Isbell, Terry A.; Murray, Rex E.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Bredsguard, Jakob W.; Roth, Katie L.; Thompson, Travis; Feken, Kati A.] Biosynthet Technol, Irvine, CA 92614 USA. RP Cermak, SC (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM steven.cermak@ars.usda.gov NR 30 TC 2 Z9 2 U1 1 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 15 PY 2015 VL 74 BP 171 EP 177 DI 10.1016/j.indcrop.2015.05.011 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA CQ9QD UT WOS:000360948900023 ER PT J AU Bufalo, J Cantrell, CL Astatkie, T Zheljazkov, VD Gawde, A Boar, CSF AF Bufalo, Jennifer Cantrell, Charles L. Astatkie, Tessema Zheljazkov, Valtcho D. Gawde, Archana Fernandes Boar, Carmen Silvia TI Organic versus conventional fertilization effects on sweet basil (Ocimum basilicum L.) growth in a greenhouse system SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Nutrients; Nitrogen; Essential oil; (-)-Linalool; Eugenol ID ESSENTIAL OIL PRODUCTION; CALLOSOBRUCHUS-MACULATUS; CHEMICAL-COMPOSITION; DRYING METHODS; YIELD; ANTIOXIDANT; CULTIVARS; NITROGEN; QUALITY; WATER AB Ocimum basilicum L. (sweet basil) is an essential oil producing crop used in culinary and fragrance applications. The objective of this controlled environment study was to evaluate the effects of organic and conventional fertilization (applied at two nitrogen rates, 150 and 250 kg N/ha) on plant growth, essential oil yield and chemical profile, and tissue nutrient accumulation in sweet basil. Overall, basil plants fertilized with organic fertilizer at a rate of 150 kg N/ha accumulated greater concentrations of potassium (K) and manganese (Mn). The highest fresh weight was obtained from the plants grown with conventional fertilizer at a rate of 250 kg N/ha. Treatments did not affect the oil content in dried biomass (0.23-0.36% range), oil yields (11.4-20.7 mg/pot), nor the concentration of eucalyptol, (-)-linalool, bornyl acetate, eugenol, alpha-trans-bergamotene, germacrene D, gamma-cadinene and epi-alpha-cadinol in basil oil. The results from this study demonstrated that organic or conventional fertilizer can alter fresh or dry weight, and nutrient absorption without modifying essential oil composition. (C) 2015 Elsevier B.V. All rights reserved. C1 [Bufalo, Jennifer; Fernandes Boar, Carmen Silvia] UNESP Univ Estadual Paulista, Inst Biosci IB, Dept Bot, BR-18618970 Sao Paulo, Brazil. [Bufalo, Jennifer; Cantrell, Charles L.; Gawde, Archana] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Astatkie, Tessema] Dalhousie Univ, Fac Agr, Truro, NS B2N 5E3, Canada. [Zheljazkov, Valtcho D.] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. RP Bufalo, J (reprint author), UNESP Univ Estadual Paulista, Inst Biosci IB, Dept Bot, POB 510, BR-18618970 Sao Paulo, Brazil. EM jenniferbufalo@yahoo.com.br RI Astatkie, Tess/J-7231-2012; OI Astatkie, Tess/0000-0002-9779-8789; Gawde, Archana/0000-0002-6495-3850 FU CAPES [4873-13]; CNPq FX Authors thank Ms. Amber Reichley and Mr. Solomon Green III, Natural Products Utilization Research Unit, USDA-ARS, for technical assistance. Thanks go to CAPES (Process 4873-13) and CNPq, for a Doctor's Degree scholarship granted to the first author. NR 50 TC 0 Z9 0 U1 15 U2 63 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 15 PY 2015 VL 74 BP 249 EP 254 DI 10.1016/j.indcrop.2015.04.032 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CQ9QD UT WOS:000360948900033 ER PT J AU White, PM Potter, TL Lima, IM AF White, Paul M., Jr. Potter, Thomas L. Lima, Isabel M. TI Sugarcane and pinewood biochar effects on activity and aerobic soil dissipation of metribuzin and pendimethalin SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Sugarcane bagasse; Pine wood; Biochar; Metribuzin; Pendimethalin; Dissipation ID DIFFERENT PYROLYTIC TEMPERATURES; MOLECULAR-STRUCTURE; ORGANIC-MATTER; SORPTION; CARBON; ADSORPTION; HERBICIDES; GROWTH; DEGRADATION; ATRAZINE AB Biochars were produced by pyrolysis of sugarcane bagasse (350 degrees C and 700 degrees C) and pine wood (400 degrees C) and are abbreviated BC350, BC700, and WC400, respectively. Metribuzin adsorption by batch equilibrium showed that BC700 had the greatest adsorption capacity followed by BC350 and WC400. The bagasse biochars were mixed with clay and a silt loam soil and the pine wood biochar with a loamy sand soil and fortified with the metribuzin and pendimethalin. The soils were incubated at 25 degrees C in the dark and were extracted with methanol after 0, 7, 14, 21, 28, 42, 63, 86, and 111 days. Parent compounds and metribuzin degradates were analyzed in extracts by HPLC- mass spectrometry. Increases in metribuzin halflife(DT50) were indicated for all soil-biochar combinations. BC350 had the greatest impact, doubling the DT50 in the silt loam soil. In contrast, BC700 had minimal impact on the DT50 in the silt loam soil and contributed to a significant DT50 decrease in the clay. The metribuzin degradate, metribuzin-desamino, was detected at rates proportional to metribuzin dissipation. For pendimethalin, biochar additions increased DT50 in all soil-biochar combinations. In contrast to metribuzin, BC700 and WC400 had the greatest impact with the BC700 increasing the DT50 more than 2-fold. In the loamy sand soil, the 1-4% biochar rate contributed to an increased trend in palmer amaranth emergence. At the 8% rate, palmer amaranth emergence was statistically higher than the control (p = 0.072). In sum, how biochar impacted the activity and dissipation of these herbicides depended on soil, herbicide, and biochar properties. Published by Elsevier B.V. C1 [White, Paul M., Jr.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [Potter, Thomas L.] USDA ARS, Southeast Watershed Res Unit, Tifton, GA 31793 USA. [Lima, Isabel M.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP White, PM (reprint author), USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. EM paul.white@ars.usda.gov; tom.potter@ars.usda.gov; isabel.lima@ars.usda.gov FU USDA Agricultural Research Service Sugarcane Research Unit in Houma, LA; Southern Regional Research Unit in New Orleans, LA; Southeast Watershed Research Unit in Tifton, GA FX The authors would like to thank Trevis Olivier, Chris Adams, Renee Bigner, Lorine Lewis, Sally Belflower, and Margie Whittle for greenhouse and laboratory assistance. Funding was provided by the USDA Agricultural Research Service Sugarcane Research Unit in Houma, LA, the Southern Regional Research Unit in New Orleans, LA, and the Southeast Watershed Research Unit in Tifton, GA. The use of trade names does not imply endorsement by the USDA. NR 43 TC 1 Z9 1 U1 8 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 15 PY 2015 VL 74 BP 737 EP 744 DI 10.1016/j.indcrop.2015.04.022 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA CQ9QD UT WOS:000360948900094 ER PT J AU Serapiglia, MJ Mullen, CA Boateng, AA Cortese, LM Bonos, SA Hoffman, L AF Serapiglia, Michelle J. Mullen, Charles A. Boateng, Akwasi A. Cortese, Laura M. Bonos, Stacy A. Hoffman, Lindsey TI Evaluation of the impact of compositional differences in switchgrass genotypes on pyrolysis product yield SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biomass composition; Bio-oil; G x E interactions; Mineral content; Pyrolysis; Switchgrass ID CATALYTIC FAST PYROLYSIS; X ENVIRONMENT INTERACTIONS; BIOMASS; FEEDSTOCK; TRAITS; LIGNIN; USA; VARIABILITY; POPULATIONS; CONVERSION AB As a dedicated bioenergy crop, switchgrass is a potential feedstock within the United States for biofuels production. It can be converted to energy dense bio-oil through fast pyrolysis. Biomass compositional differences can influence the conversion efficiency and bio-oil product yield and quality. In order to understand how improvements in bio-oil quality can be achieved by manipulation of biomass composition, differences in switchgrass biomass composition were evaluated for their impacts on fast pyrolysis product yield. Nine genotypes of switchgrass were grown on one prime and two marginal sites in New Jersey. The results show that biomass composition was affected by genotype, the environment, and genotype x environment interactions. Non-catalytic pyrolysis product yields were largely affected by genotypic differences. The most significant impacts on non-catalytic pyrolysis products were from cellulose content and K content in the biomass. It was found that non-methoxylated phenolics were mainly produced from the breakdown of levoglucosan in the presence of K. Mineral content in the biomass was highly variable by environment and soil variability across the sites examined. These differences in mineral content largely impacted product distribution of HZSM-5-catalyzed pyrolysis, showing that lower mineral uptake in the biomass was beneficial for the production of aromatic hydrocarbons. Significant genotypic and environmental effects among the pyrolysis products demonstrate that breeding for improvements in pyrolysis product yield is conceivable but that growing conditions and soil conditions must also be taken into consideration. Published by Elsevier B.V. C1 [Serapiglia, Michelle J.; Mullen, Charles A.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Cortese, Laura M.; Bonos, Stacy A.; Hoffman, Lindsey] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. RP Boateng, AA (reprint author), ARS, Sustainable Biofuels & Coprod Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov FU USDA-NIFA grant [2012-68005-19703] FX Funding for this project was provided by a USDA-NIFA grant (#2012-68005-19703) to the Northeast Woody/Warm Season Biomass Consortium (NEWBio). The authors would like to thank Mary Cheetham for her excellent technical assistance and data collection and analysis on this project. NR 30 TC 7 Z9 7 U1 4 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD NOV 15 PY 2015 VL 74 BP 957 EP 968 DI 10.1016/j.indcrop.2015.06.024 PG 12 WC Agricultural Engineering; Agronomy SC Agriculture GA CQ9QD UT WOS:000360948900120 ER PT J AU Aldrich, PR El-Zabet, J Hassan, S Briguglio, J Aliaj, E Radcliffe, M Mirza, T Comar, T Nadolski, J Huebner, CD AF Aldrich, Preston R. El-Zabet, Jermeen Hassan, Seerat Briguglio, Joseph Aliaj, Enela Radcliffe, Maria Mirza, Taha Comar, Timothy Nadolski, Jeremy Huebner, Cynthia D. TI Monte Carlo tests of small-world architecture for coarse-grained networks of the United States railroad and highway transportation systems SO PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIONS LA English DT Article DE Highway; Network; Railroad; Small-world; Transportation ID SCALE-FREE; WEIGHTED NETWORKS; RAILWAY NETWORK; GLOBALIZATION; INVASIONS; CHINA; ERA AB Several studies have shown that human transportation networks exhibit small-world structure, meaning they have high local clustering and are easily traversed. However, some have concluded this without statistical evaluations, and others have compared observed structure to globally random rather than planar models. Here, we use Monte Carlo randomizations to test US transportation infrastructure data for small-worldness. Coarse-grained network models were generated from GIS data wherein nodes represent the 3105 contiguous US counties and weighted edges represent the number of highway or railroad links between counties; thus, we focus on linkage topologies and not geodesic distances. We compared railroad and highway transportation networks with a simple planar network based on county edge-sharing, and with networks that were globally randomized and those that were randomized while preserving their planarity. We conclude that terrestrial transportation networks have small-world architecture, as it is classically defined relative to global randomizations. However, this topological structure is sufficiently explained by the planarity of the graphs, and in fact the topological patterns established by the transportation links actually serve to reduce the amount of small-world structure. (C) 2015 Elsevier B.V. All rights reserved. C1 [Aldrich, Preston R.; El-Zabet, Jermeen; Hassan, Seerat; Briguglio, Joseph; Aliaj, Enela; Radcliffe, Maria; Mirza, Taha] Benedictine Univ, Dept Biol Sci, Lisle, IL 60532 USA. [Comar, Timothy; Nadolski, Jeremy] Benedictine Univ, Dept Math & Computat Sci, Lisle, IL USA. [Huebner, Cynthia D.] US Forest Serv, No Res Stn, USDA, Morgantown, WV USA. RP Aldrich, PR (reprint author), Benedictine Univ, Dept Biol Sci, Lisle, IL 60532 USA. EM paldrich@ben.edu FU USDA Forest Products Laboratory [04-JV-11242328-119, 05-JV-11242343-057, 06-JV-11242343-057]; Illinois Bord of Higher Education [05S001270001]; COS Summer Research Program at Benedictine University FX We thank the US Department of Transportation, the Federal Highway Administration, the Federal Railroad Administration, and the US Census Bureau for use of their data. This work was supported in part by grants from the USDA Forest Products Laboratory [04-JV-11242328-119, 05-JV-11242343-057, and 06-JV-11242343-057 to PA and GG], Illinois Bord of Higher Education [05S001270001 to PA], and the COS Summer Research Program at Benedictine University. The authors declare no conflict of interest. NR 42 TC 0 Z9 0 U1 2 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4371 EI 1873-2119 J9 PHYSICA A JI Physica A PD NOV 15 PY 2015 VL 438 BP 32 EP 39 DI 10.1016/j.physa.2015.06.013 PG 8 WC Physics, Multidisciplinary SC Physics GA CQ8PQ UT WOS:000360871200004 ER PT J AU Chavez, E He, ZL Stoffella, PJ Mylavarapu, RS Li, YC Moyano, B Baligar, VC AF Chavez, E. He, Z. L. Stoffella, P. J. Mylavarapu, R. S. Li, Y. C. Moyano, B. Baligar, V. C. TI Concentration of cadmium in cacao beans and its relationship with soil cadmium in southern Ecuador SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Anthropogenic activities; Cadmium distribution; Chemical extraction; Plant-availability; Soil contamination ID TRACE-ELEMENTS; HEAVY-METALS; PHOSPHATE FERTILIZERS; ENVIRONMENTAL RISKS; AGRICULTURAL SOILS; POLLUTED SOILS; LEAD; NICKEL; COPPER; BIOAVAILABILITY AB Cadmium(Cd) content in cacao beans above a critical level (0.6mg kg(-1)) has raised concerns in the consumption of cacao-based chocolate. Little is available regarding Cd concentration in soil and cacao in Ecuador. The aim of this study was to determine the status of Cd in both, soils and cacao plants, in southern Ecuador. Soil samples were collected from 19 farms at 0-5, 5-15, 15-30, and 30-50 cm depths, whereas plant samples were taken from four nearby trees. Total recoverable and extractable Cd were measured at the different soil depths. Total recoverable Cd ranged from 0.88 to 2.45 and 0.06 to 2.59, averaged 1.54 and 0.85 mg kg(-1), respectively in the surface and subsurface soils whereas the corresponding values for M3-extractable Cd were 0.08 to 1.27 and 0.02 to 0.33 with mean values of 0.40 and 0.10 mg kg(-1). Surface soil in all sampling sites had total recoverable Cd above the USEPA critical level for agricultural soils (0.43 mg kg(-1)), indicating that Cd pollution occurs. Since both total recoverable and M3-extractable Cd significantly decreased depth wise, anthropogenic activities are more likely the source of contamination. Cadmium in cacao tissues decreased in the order of beans > shell > > leaves. Cadmium content in cacao beans ranged from 0.02 to 3.00, averaged 0.94 mg kg(-1), and 12 out of 19 sites had bean Cd content above the critical level. Bean Cd concentration was highly correlated with M3- or HCl-extractable Cd at both the 0-5and5-15cmdepths (r=0.80 and 0.82 for M3, and r=0.78 and 0.82 for HCl; P < 0.01). These results indicate that accumulation of Cd in surface layers results in excessive Cd in cacao beans and M3- or HCl-extractable Cd are suitable methods for predicting available Cd in the studied soils. (C) 2015 Elsevier B.V. All rights reserved. C1 [Chavez, E.; He, Z. L.; Stoffella, P. J.] Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. [Mylavarapu, R. S.] Univ Florida, Inst Food & Agr Sci, Soil & Water Sci Dept, Gainesville, FL 33611 USA. [Li, Y. C.] Univ Florida, Inst Food & Agr Sci, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Chavez, E.; Moyano, B.] Escuela Super Politecn Litoral, Ctr Invest Biotecnol Ecuador, Guayaquil, Guayas, Ecuador. [Baligar, V. C.] ARS, USDA, Beltsville, MD 20705 USA. RP He, ZL (reprint author), Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. EM zhe@ufl.edu RI He, Zhenli/R-1494-2016 OI He, Zhenli/0000-0001-7761-2070 NR 60 TC 6 Z9 6 U1 15 U2 98 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD NOV 15 PY 2015 VL 533 BP 205 EP 214 DI 10.1016/j.scitotenv.2015.06.106 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CQ0LV UT WOS:000360288300024 PM 26172587 ER PT J AU Pinto, VZ Vanier, NL Deon, VG Moomand, K El Halal, SLM Zavareze, ED Lim, LT Dias, ARG AF Pinto, Vania Zanella Vanier, Nathan Levien Deon, Vinicius Goncalves Moomand, Khalid Mello El Halal, Shanise Lisie Zavareze, Elessandra da Rosa Lim, Loong-Tak Guerra Dias, Alvaro Renato TI Effects of single and dual physical modifications on pinhao starch SO FOOD CHEMISTRY LA English DT Article DE Annealing; Araucaria angustifolia Bert O. Ktze; Heat-moisture-treatment; Sonication; Starch structure; Relative crystallinity; Viscosity ID HEAT-MOISTURE TREATMENT; DIFFERENT BOTANICAL SOURCES; TREATED POTATO STARCH; PHYSICOCHEMICAL PROPERTIES; MOLECULAR-STRUCTURE; CORN STARCH; THERMAL-PROPERTIES; X-RAY; ULTRASOUND; IMPACT AB Pinhao starch was modified by annealing (ANN), heat-moisture (HMT) or sonication (SNT) treatments. The starch was also modified by a combination of these treatments (ANN-HMT, ANN-SNT, HMT-ANN, HMT-SNT, SNT-ANN, SNT-HMT). Whole starch and debranched starch fractions were analyzed by gel-permeation chromatography. Moreover, crystallinity, morphology, swelling power, solubility, pasting and gelatinization characteristics were evaluated. Native and single ANN and SNT-treated starches exhibited a CA-type crystalline structure while other modified starches showed an A-type structure. The relative crystallinity increased in ANN-treated starches and decreased in single HMT- and SNT-treated starches. The ANN, HMT and SNT did not provide visible cracks, notches or grooves to pinhao starch granule. SNT applied as second treatment was able to increase the peak viscosity of single ANN- and HMT-treated starches. HMT used alone or in dual modifications promoted the strongest effect on gelatinization temperatures and enthalpy. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Pinto, Vania Zanella; Vanier, Nathan Levien; Mello El Halal, Shanise Lisie; Zavareze, Elessandra da Rosa; Guerra Dias, Alvaro Renato] Univ Fed Pelotas, Dept Ciencia & Tecnol Agroind, BR-96010900 Pelotas, RS, Brazil. [Pinto, Vania Zanella; Moomand, Khalid; Lim, Loong-Tak] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada. [Vanier, Nathan Levien] ARS, Hlth Proc Foods Res Unit, WRRC, USDA, Albany, CA 94710 USA. [Deon, Vinicius Goncalves] Univ Fed Pelotas, Ctr Desenvolvimento Tecnol, Engn Mat, BR-96010900 Pelotas, RS, Brazil. RP Pinto, VZ (reprint author), Univ Fed Pelotas, Dept Ciencia & Tecnol Agroind, BR-96010900 Pelotas, RS, Brazil. EM vania_vzp@hotmail.com OI Zanella Pinto, Vania/0000-0002-7081-5446 FU CAPES; FAPERGS; CNPq; SCT-RS; Ciencia sem Fronteiras (CsF) program FX We would like to thank CAPES, FAPERGS, CNPq, SCT-RS and Ciencia sem Fronteiras (CsF) program for financial support and the scholarships awarded. We also would like to thank Dr. Koushik Seetharaman (in memoriam) and his team for starch molecular structure analyses and CEME-Sul-FURG, both for assistance in results interpretation. NR 42 TC 7 Z9 7 U1 5 U2 62 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 15 PY 2015 VL 187 BP 98 EP 105 DI 10.1016/j.foodchem.2015.04.037 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CK0HD UT WOS:000355885400014 PM 25977003 ER PT J AU Shepherd, LVT Hackett, CA Alexander, CJ McNicol, JW Sungurtas, JA Stewart, D Mccue, KF Belknap, WR Davies, HV AF Shepherd, Louise Vida Trail Hackett, Christine Anne Alexander, Colin James McNicol, James William Sungurtas, Julia Anne Stewart, Derek McCue, Kent Frank Belknap, William Richardson Davies, Howard Vivian TI Modifying glycoalkaloid content in transgenic potato - Metabolome impacts SO FOOD CHEMISTRY LA English DT Article DE Genetic modification; Glycoalkaloids; Metabolomics; Potato (Solanum tuberosum L.); SGT; Transgenic ID SOLANIDINE GLUCOSYLTRANSFERASE; SOLANUM-TUBEROSUM; PLANTS; TRANSFORMATION; CHOLESTEROL; EXPRESSION AB Metabolite profiling has been used to assess the potential for unintended composition changes in potato (Solanum tuberosum L. cv. Desiree) tubers, which have been genetically modified (GM) to reduce glycoalkaloid content, via the independent down-regulation of three genes SGT1, SGT2 and SGT3 known to be involved in glycoalkaloid biosynthesis. Differences between the three groups of antisense lines and control lines were assessed using liquid chromatography mass spectrometry (LC-MS) and gas chromatography (GC) MS, and data analysed using principal component analysis and analysis of variance. Compared with the wild-type (WT) control, LC-MS revealed not only the expected changes in specific glycoalkaloid levels in the GM lines, but also significant changes in several other metabolites, some of which were explicable in terms of known pathways. Analysis of polar and non-polar metabolites by GC-MS revealed other significant (unintended) differences between SGT lines and the WT, but also between the WT control and other control lines used. (C) 2015 Published by Elsevier Ltd. C1 [Shepherd, Louise Vida Trail; Sungurtas, Julia Anne; Stewart, Derek; Davies, Howard Vivian] James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. [Hackett, Christine Anne; Alexander, Colin James; McNicol, James William] Biomath & Stat Scotland, Dundee DD2 5DA, Scotland. [Stewart, Derek] Heriot Watt Univ, Sch Life Sci, Edinburgh EH14 4AS, Midlothian, Scotland. [McCue, Kent Frank; Belknap, William Richardson] ARS, USDA, Crop Improvement & Genet, Western Reg Res Ctr, Albany, CA 94710 USA. RP Shepherd, LVT (reprint author), James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. EM louise.shepherd@hutton.ac.uk RI Alexander, Colin/C-5802-2009 OI Alexander, Colin/0000-0003-4995-1678 FU SAFEFOODS (EU FP6) [Food-CT-2004-506446]; Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division FX This work was supported by SAFEFOODS (EU FP6 Contract No Food-CT-2004-506446) and by the Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division. NR 39 TC 4 Z9 4 U1 3 U2 89 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 15 PY 2015 VL 187 BP 437 EP 443 DI 10.1016/j.foodchem.2015.04.111 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CK0HD UT WOS:000355885400059 PM 25977048 ER PT J AU Jang, A Bae, W Hwang, HS Lee, HG Lee, S AF Jang, Areum Bae, Woosung Hwang, Hong-Sik Lee, Hyeon Gyu Lee, Suyong TI Evaluation of canola oil oleogels with candelilla wax as an alternative to shortening in baked goods SO FOOD CHEMISTRY LA English DT Article DE Oleogel; Canola oil; Candelilla wax; Shortening; Cookie ID RHEOLOGICAL PROPERTIES; VEGETABLE-OILS; ORGANOGELS; FAT AB The oleogels of canola oil with candelilla wax were prepared and utilized as a shortening replacer to produce cookies with a high level of unsaturated fatty acids. The incorporation of candelilla wax (3% and 6% by weight) to canola oil produced the oleogels with solid-like properties. The firmness of the oleogels was lower than that of the shortening at room temperature. A more rapid change in the viscosity with temperature was observed with increasing levels of candelilla wax in the steady shear measurements. The replacement of shortening with oleogels in the cookie formulation reduced both viscoelastic parameters (G' and G '') of the cookie doughs. The level of unsaturated fatty acids in the oleogel cookies was distinctly increased up to around 92%, compared to the shortening cookies (47.2%). The cookies with the oleogels showed desirable spreadable property and the replacement of shortening with the oleogels produced cookies with soft eating characteristics. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Jang, Areum; Bae, Woosung; Lee, Suyong] Sejong Univ, Dept Food Sci & Technol, Seoul 143747, South Korea. [Jang, Areum; Bae, Woosung; Lee, Suyong] Sejong Univ, Carbohydrate Bioprod Res Ctr, Seoul 143747, South Korea. [Hwang, Hong-Sik] ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL USA. [Lee, Hyeon Gyu] Hanyang Univ, Dept Food & Nutr, Seoul 133791, South Korea. RP Lee, S (reprint author), Sejong Univ, Dept Food Sci & Technol, 98 Gunja Dong, Seoul 143747, South Korea. EM suyonglee@sejong.ac.kr FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2013R1A1A2A10004640] FX This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A2A10004640). NR 20 TC 13 Z9 13 U1 8 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD NOV 15 PY 2015 VL 187 BP 525 EP 529 DI 10.1016/j.foodchem.2015.04.110 PG 5 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CK0HD UT WOS:000355885400070 PM 25977059 ER PT J AU Jacques, PF Cassidy, A Rogers, G Peterson, JJ Dwyer, JT AF Jacques, Paul F. Cassidy, Aedin Rogers, Gail Peterson, Julia J. Dwyer, Johanna T. TI Dietary flavonoid intakes and CVD incidence in the Framingham Offspring Cohort SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE CVD; Diet; Flavonoid intake; Observational study; Stroke ID CORONARY-HEART-DISEASE; FOOD FREQUENCY QUESTIONNAIRE; MALE HEALTH-PROFESSIONALS; CARDIOVASCULAR-DISEASE; VEGETABLE CONSUMPTION; ANTIOXIDANT FLAVONOLS; MYOCARDIAL-INFARCTION; POSTMENOPAUSAL WOMEN; MEDITERRANEAN DIET; BLOOD-PRESSURE AB This study examines the relationship between long-term intake of six flavonoid classes and incidence of CVD and CHD, using a comprehensive flavonoid database and repeated measures of intake, while accounting for possible confounding by components of a healthy dietary pattern. Flavonoid intakes were assessed using a FFQ among the Framingham Offspring Cohort at baseline and three times during follow-up. Cox proportional hazards regression was used to characterise prospective associations between the natural logarithms of flavonoid intakes and CVD incidence using a time-dependent approach, in which intake data were updated at each examination to represent average intakes from previous examinations. Mean baseline age was 54 years, and 45 % of the population was male. Over an average 14.9 years of follow-up among 2880 participants, there were 518 CVD events and 261 CHD events. After multivariable adjustment, only flavonol intake was significantly associated with lower risk of CVD incidence (hazard ratios (HR) per 2.5-fold flavonol increase=0.86, P-trend=0.05). Additional adjustment for total fruit and vegetable intake and overall diet quality attenuated this observation (HR=0.89, P-trend=0.20 and HR=0.92, P-trend=0.33, respectively). There were no significant associations between flavonoids and CHD incidence after multivariable adjustment. Our findings suggest that the observed association between flavonol intake and CVD risk may be a consequence of better overall diet. However, the strength of this non-significant association was also consistent with relative risks observed in previous meta-analyses, and therefore a modest benefit of flavonol intake on CVD risk cannot be ruled out. C1 [Jacques, Paul F.; Rogers, Gail; Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, Paul F.; Peterson, Julia J.; Dwyer, Johanna T.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Cassidy, Aedin] Univ E Anglia, Norwich Med Sch, Dept Nutr, Norwich NR4 7UQ, Norfolk, England. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Dept Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Dept Community Hlth, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Med Ctr, Frances Stern Nutr Ctr, Boston, MA 02111 USA. RP Jacques, PF (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM paul.jacques@tufts.edu OI Dwyer, Johanna/0000-0002-0783-1769 FU International Life Sciences Institute North America; National Heart Lung and Blood Institute [N01-HC-25195]; Biotechnology and Biological Sciences Research Council (BBSRC), UK [BB/J004545/1]; USDA Agricultural Research Service [58-1950-0-014] FX The present study was supported by grants from the International Life Sciences Institute North America, the National Heart Lung and Blood Institute (contract N01-HC-25195), the Biotechnology and Biological Sciences Research Council (BBSRC), UK (REF: BB/J004545/1), and the USDA Agricultural Research Service (agreement 58-1950-0-014). A. C. is a Royal Society Wolfson Research Merit Award Holder. NR 50 TC 4 Z9 4 U1 0 U2 8 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD NOV 14 PY 2015 VL 114 IS 9 BP 1496 EP 1503 DI 10.1017/S0007114515003141 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CV8IL UT WOS:000364526600019 PM 26334117 ER PT J AU Nussle, S Matthews, KR Carlson, SM AF Nussle, Sebastien Matthews, Kathleen R. Carlson, Stephanie M. TI Mediating Water Temperature Increases Due to Livestock and Global Change in High Elevation Meadow Streams of the Golden Trout Wilderness SO PLOS ONE LA English DT Article ID RECENT CLIMATE-CHANGE; JUVENILE COHO SALMON; EVOLUTIONARY RESPONSES; PACIFIC-NORTHWEST; EXTINCTION RISK; RAINBOW-TROUT; UNITED-STATES; CALIFORNIA; RESTORATION; MANAGEMENT AB Rising temperatures due to climate change are pushing the thermal limits of many species, but how climate warming interacts with other anthropogenic disturbances such as land use remains poorly understood. To understand the interactive effects of climate warming and livestock grazing on water temperature in three high elevation meadow streams in the Golden Trout Wilderness, California, we measured riparian vegetation and monitored water temperature in three meadow streams between 2008 and 2013, including two "resting" meadows and one meadow that is partially grazed. All three meadows have been subject to grazing by cattle and sheep since the 1800s and their streams are home to the imperiled California golden trout (Oncorhynchus mykiss aguabonita). In 1991, a livestock exclosure was constructed in one of the meadows (Mulkey), leaving a portion of stream ungrazed to minimize the negative effects of cattle. In 2001, cattle were removed completely from two other meadows (Big Whitney and Ramshaw), which have been in a "resting" state since that time. Inside the livestock exclosure in Mulkey, we found that riverbank vegetation was both larger and denser than outside the exclosure where cattle were present, resulting in more shaded waters and cooler maximal temperatures inside the exclosure. In addition, between meadows comparisons showed that water temperatures were cooler in the ungrazed meadows compared to the grazed area in the partially grazed meadow. Finally, we found that predicted temperatures under different global warming scenarios were likely to be higher in presence of livestock grazing. Our results highlight that land use can interact with climate change to worsen the local thermal conditions for taxa on the edge and that protecting riparian vegetation is likely to increase the resiliency of these ecosystems to climate change. C1 [Nussle, Sebastien; Carlson, Stephanie M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Matthews, Kathleen R.] US Forest Serv, Pacific Southwest Res Stn, USDA, Albany, CA USA. RP Nussle, S (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM snussle@gmail.com FU USDA Pacific Southwest Research Station; National Fish and Wildlife Foundation [3017]; Sierra Flyfishers; Swiss National Science Foundation [P2LAP3_148434] FX The project and KRM were funded by the USDA Pacific Southwest Research Station (http://www.fs.fed.us/psw/), National Fish and Wildlife Foundation (Bring Back the Natives Grant #3017) (http://www.nfwf.org/bbn), and the Sierra Flyfishers. SN was funded by the Swiss National Science Foundation (P2LAP3_148434) (www.snf.ch). NR 77 TC 0 Z9 0 U1 6 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 13 PY 2015 VL 10 IS 11 AR e0142426 DI 10.1371/journal.pone.0142426 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DA2MI UT WOS:000367628500022 PM 26565706 ER PT J AU Pereira, AE Wang, HC Zukoff, SN Meinke, LJ French, BW Siegfried, BD AF Pereira, Adriano E. Wang, Haichuan Zukoff, Sarah N. Meinke, Lance J. French, B. Wade Siegfried, Blair D. TI Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas SO PLOS ONE LA English DT Article ID CHRYSOMELIDAE LARVAL INJURY; ERROR COMPONENT MODEL; BACILLUS-THURINGIENSIS; METHYL-PARATHION; CROP-ROTATION; COLEOPTERA; INSECTICIDES; SUSCEPTIBILITY; MAIZE; ADAPTATION AB Pyrethroid insecticides have been used to control larvae or adults of the western corn root-worm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and used to test adults of both laboratory and field populations. Larval susceptibility was also tested using both laboratory and field populations. Significant differences were recorded in adult and larval susceptibility among WCR field and laboratory populations. The highest LC50 for WCR adults was observed in populations from Keith 2 and Chase Counties, NE, with LC(50)s of 2.2 and 1.38 mu g/vial, respectively, and Finney County 1, KS, with 1.43 mu g/vial, as compared to a laboratory non-diapause population (0.24 mu g/vial). For larvae, significant differences between WCR field and laboratory populations were also recorded. Significant differences in mortalities at the diagnostic bifenthrin concentration (LC99) were observed among WCR adult populations with western Corn Belt populations exhibiting lower susceptibility to bifenthrin, especially in southwestern Nebraska and southwestern Kansas. This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides. Implications to sustainable rootworm management are discussed. C1 [Pereira, Adriano E.; Wang, Haichuan; Meinke, Lance J.; Siegfried, Blair D.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Zukoff, Sarah N.] Kansas State Univ, Southwest Res & Extens Ctr, Garden City, KS USA. [French, B. Wade] ARS, North Cent Agr Res Lab, USDA, Brookings, SD USA. RP Pereira, AE (reprint author), Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. EM aelias374@yahoo.com.br FU Monsanto Corn Rootworm Knowledge Grant [69292]; Department of Entomology/Institute of Agriculture and Natural Resources FX This work was supported by: 1- Monsanto Corn Rootworm Knowledge Grant (No. 69292): The recipients were BDS and LJM. Monsanto staff provided some field insect collections. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. 2- Department of Entomology/Institute of Agriculture and Natural Resources: The recipient was AEP as an assistantship for the PhD program. NR 54 TC 5 Z9 5 U1 3 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 13 PY 2015 VL 10 IS 11 AR e0142299 DI 10.1371/journal.pone.0142299 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DA2MI UT WOS:000367628500017 PM 26566127 ER PT J AU Joshi, NK Rajotte, EG Myers, CT Krawczyk, G Hull, LA AF Joshi, Neelendra K. Rajotte, Edwin G. Myers, Clayton T. Krawczyk, Greg Hull, Larry A. TI Development of a Susceptibility Index of Apple Cultivars for Codling Moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae) Oviposition SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE apple cultivars; codling moth; oviposition; susceptibility; host preference; Honeycrisp; Gala; Golden Delicious ID LASPEYRESIA-POMONELLA; ALPHA-FARNESENE; CHEMICAL ECOLOGY; INSECT PESTS; FRUIT; WALNUT; OLETHREUTIDAE; RESISTANCE; VARIETIES; BEHAVIOR AB Codling moth (CM), Cydia pomonella (L.) (Lepidoptera: Tortricidae) is a major fruit feeding pest of apples. Understanding susceptibility differences of various apple cultivars to CM oviposition is an important step in developing resistant varieties as well as monitoring and management strategies for this pest in apple orchards planted with mixed-cultivars. In this context, oviposition preferences of CM for the fruits of different apple cultivars were studied in laboratory bioassays using a series of no choice and multiple-choice tests in 2006, 2007, and 2008. In 2006 and 2007, 10 apple cultivars, viz., Arlet, Fuji, Gala, Golden Delicious, Honeycrisp, Pristine, Delicious, Stayman, Sunrise, and York Imperial were evaluated, while in the 2008 tests, Golden Delicious, Honeycrisp, and York Imperial were evaluated. During the 2006 tests, preferred apple cultivars for CM oviposition were Golden Delicious and Fuji, while the least preferred were Arlet, Pristine, Sunrise, and Honeycrisp. Similarly, during the 2007 tests, Golden Delicious, Fuji and Stayman remained the preferred cultivars, while Arlet, Honeycrisp, Pristine, and Sunrise remained the least preferred cultivars. In the 2008 tests, Golden Delicious and Honeycrisp were the most and least preferred cultivars, respectively. Based on the oviposition preferences from these bioassays, a susceptibility index for each cultivar was developed. This index may be used as a standard measure in cultivar evaluations in breeding programs, and may assist fruit growers and crop consultants to select the most appropriate cultivar(s) for monitoring and detecting the initial signs of fruit injury from CM in an apple orchard planted with mixed-cultivars. C1 [Joshi, Neelendra K.; Rajotte, Edwin G.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Joshi, Neelendra K.; Krawczyk, Greg; Hull, Larry A.] Penn State Univ, Entomol Fruit Res & Extens Ctr, Biglerville, PA USA. [Myers, Clayton T.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. RP Joshi, NK (reprint author), Univ Arkansas, Dept Entomol, 319 Agr Bldg, Fayetteville, AR 72701 USA. EM nkj105@psu.edu FU State Horticultural Association of Pennsylvania; Garretson Endowment; Pennsylvania State University Libraries Open Access Publishing Fund FX The authors would like to thank the State Horticultural Association of Pennsylvania and the Garretson Endowment for providing funding, and Teresa Krawczyk, Fruit Research and Extension Center- Penn State, for providing the insect colony. The authors are also thankful to the Statistical Consulting Center, Department of Statistics at Penn State University for their assistance with the statistical analyses and consultation, and Dr. Robert M. Crassweller (Penn State University) and Dr. James Schupp (Penn State Fruit Research and Extension Center) for providing fruits of certain cultivars. The authors are also thankful to Dr. James Schupp and Dr. Dennis Calvin for their insightful comments on the previous version of this manuscript. Publication of this article was funded in part by The Pennsylvania State University Libraries Open Access Publishing Fund. NR 51 TC 0 Z9 0 U1 4 U2 28 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD NOV 13 PY 2015 VL 6 AR 992 DI 10.3389/fpls.2015.00992 PG 13 WC Plant Sciences SC Plant Sciences GA CY2QO UT WOS:000366253400001 PM 26617629 ER PT J AU Liu, LF Dang, PM Chen, CY AF Liu, Lifeng Dang, Phat M. Chen, Charles Y. TI Development and Utilization of InDel Markers to Identify Peanut (Arachis hypogaea) Disease Resistance SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE InDel markers; cultivated peanut; genetic diversity; disease resistances ID MINI-CORE COLLECTION; POLYMORPHIC DNA MARKERS; GENETIC-LINKAGE MAP; HIGH OLEATE TRAIT; CULTIVATED PEANUT; NEMATODE RESISTANCE; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; ASSOCIATION ANALYSIS; L. AB Peanut diseases, such as leaf spot and spotted wilt caused by Tomato Spotted Wilt Virus, can significantly reduce yield and quality. Application of marker assisted plant breeding requires the development and validation of different types of DNA molecular markers. Nearly 10,000 SSR-based molecular markers have been identified by various research groups around the world, but less than 14.5% showed polymorphism in peanut and only 6.4% have been mapped. Low levels of polymorphism limit the application of marker assisted selection (MAS) in peanut breeding programs. Insertion/deletion (InDel) markers have been reported to be more polymorphic than SSRs in some crops. The goals of this study were to identify novel InDel markers and to evaluate the potential use in peanut breeding. Forty-eight InDel markers were developed from conserved sequences of functional genes and tested in a diverse panel of 118 accessions covering six botanical types of cultivated peanut, of which 104 were from the U.S. mini-core. Results showed that 16 InDel markers were polymorphic with polymorphic information content (PIG) among InDels ranged from 0.017 to 0.660. With respect to botanical types, PICs varied from 0.176 for fastigiata var., 0.181 for hypogaea var., 0.306 for vulgaris var., 0.534 for aequatoriana var., 0.556 for peruviana var., to 0.660 for hirsuta var., implying that aequatoriana var., peruviana var., and hirsuta var. have higher genetic diversity than the other types and provide a basis for gene functional studies. Single marker analysis was conducted to associate specific marker to disease resistant traits. Five InDels from functional genes were identified to be significantly correlated to tomato spotted wilt virus (TSWV) infection and leaf spot, and these novel markers will be utilized to identify disease resistant genotype in breeding populations. C1 [Liu, Lifeng; Chen, Charles Y.] Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. [Liu, Lifeng] Agr Univ Hebei, Dept Agron, Baoding, Peoples R China. [Dang, Phat M.] USDA ARS, Natl Peanut Res Lab, Dawson, GA 31742 USA. RP Chen, CY (reprint author), Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. EM cyc0002@auburn.edu FU 948 project [2013-Z65]; Excellent Going Abroad Experts Training Program in Hebei, China FX We are indebted to Brian Gamble and Larry Wells for devoted assistance with management of field experiment research plots at the Wiregrass Research and Extension Center, Auburn University, Headland, Alabama. The contributions and assistances of Sam Hilton, Joseph Powell, Kathy Gray, Lori Riles, Dan Todd, Robin Barfield, Staci Ingram, and Bill Edwards from the USDA-ARS National Peanut Research Laboratory are gratefully acknowledged. The author, LL was sponsored by Grant of 948 project (2013-Z65) and The Excellent Going Abroad Experts Training Program in Hebei, China to conduct this research in Auburn University. NR 56 TC 2 Z9 2 U1 4 U2 12 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD NOV 13 PY 2015 VL 6 AR 988 DI 10.3389/fpls.2015.00988 PG 11 WC Plant Sciences SC Plant Sciences GA CY2QM UT WOS:000366253200002 PM 26617627 ER PT J AU Codling, EE Lewis, J Watts, DB AF Codling, Eton E. Lewis, Jessica Watts, Dexter B. TI Broiler Litter Ash and Flue Gas Desulfurization Gypsum Effects on Peanut Yield and Uptake of Nutrients SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE Ash; by-products; litter; peanuts ID POULTRY LITTER; VEGETATIVE GROWTH; SOIL CALCIUM; PLANT UPTAKE; BY-PRODUCTS; MANURE ASH; PHOSPHORUS; CULTIVARS; IRRIGATION; COMBUSTION AB Peanut (Arachis hypogaea L.) requires calcium (Ca) and phosphorus (P). Flue gas desulfurization gypsum (FGDG) and broiler litter ash (BLA) could be used as Ca and P fertilizer for peanuts. A pot study was conducted to determine the effects of BLA and FGDG on peanut yield and nutrient uptake compared to superphosphate (SP). Peanut kernel yields were 13.5 greater with BLA compared to SP. Kernel Ca concentration was 29% and P was 17% lower in in plants grown on the BLA compared to SP. Because of the high Ca content of the amended soil no significant difference was observed when FGDG was added. Micronutrients in kernel fertilized with BLA and FGDG were similar to superphosphate. The small difference in kernel Ca and P and the low levels of metals in the tissue between the BLA and SP demonstrate that BLA could be used as P source for peanuts. C1 [Codling, Eton E.] USDA ARS, Crop Syst Global Change Lab, Beltsville, MD 20705 USA. [Lewis, Jessica] Univ Maryland, Sch Business, College Pk, MD 20742 USA. [Watts, Dexter B.] USDA ARS, Soil Dynam Lab, Auburn, AL USA. RP Codling, EE (reprint author), USDA ARS, Crop Syst Global Change Lab, Beltsville, MD 20705 USA. EM eton.codling@ars.usda.gov NR 53 TC 0 Z9 0 U1 0 U2 14 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD NOV 13 PY 2015 VL 46 IS 20 BP 2553 EP 2575 DI 10.1080/00103624.2015.1085553 PG 23 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA CV5NF UT WOS:000364316900003 ER PT J AU Ramadugu, C Keremane, ML Hu, XL Karp, D Federici, CT Kahn, T Roose, ML Lee, RF AF Ramadugu, Chandrika Keremane, Manjunath L. Hu, Xulan Karp, David Federici, Claire T. Kahn, Tracy Roose, Mikeal L. Lee, Richard F. TI Genetic analysis of citron (Citrus medica L.) using simple sequence repeats and single nucleotide polymorphisms SO SCIENTIA HORTICULTURAE LA English DT Article DE Citron; Microsatellite analysis; Single nucleotide polymorphism; Population structure ID ORANGE SUBFAMILY AURANTIOIDEAE; MULTILOCUS GENOTYPE DATA; PHYLOGENETIC-RELATIONSHIPS; CHLOROPLAST DNA; MICROSATELLITE MARKERS; POPULATION-STRUCTURE; PCR-RFLP; MOLECULAR PHYLOGENY; DIVERSITY; RUTACEAE AB Citron (Citrus medica L.) is one of the three basic species of the genus Citrus L. that have contributed to the development of cultivated citrus. We analyzed the genetic diversity of 47 citrons (32 from Yunnan Province, China; and 15 of Mediterranean origin) to understand their diversity and relationships within the species. Genetic analysis was conducted using data from microsatellite markers, single nucleotide polymorphisms generated from sequences of a nuclear malate dehydrogenase gene and a chloroplast gene, rps16. Neighbor joining and maximum parsimony analyses were conducted. All three approaches found citron to be monophyletic. Population structure analysis clustered the 47 citrons into three distinct groups. The first group consisted of wild, non-fingered citrons generally having locules, juice sacs and seeds within the fruit. The second cluster consisted mostly of fingered citrons that lacked locules, juice sacs or seeds, and some non-fingered types with smaller locules and vestigial juice sacs, but with seeds. All accessions that clustered in groups I and II originated in China. The third cluster consisted of citrons cultivated in the Mediterranean region. Genetic distance between the clusters from population structure analysis indicated considerable diversity within the species. A citron-specific microsatellite marker was identified and characterized. We observed considerable heterozygosity in certain citrons, contrary to previous reports. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Ramadugu, Chandrika; Karp, David; Federici, Claire T.; Kahn, Tracy; Roose, Mikeal L.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Keremane, Manjunath L.; Lee, Richard F.] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. [Hu, Xulan] Yunnan Prov Dept Agr, Kunming, Yunnan, Peoples R China. RP Ramadugu, C (reprint author), Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. EM chandram@ucr.edu; Manjunath.keremane@ars.usda.gov; xulan.hu@163.com; david.karp@ucr.edu; claire.federici@ucr.edu; tracy.kahn@ucr.edu; Mikeal.roose@ucr.edu; rfleevirus@yahoo.com FU University of California Riverside; United States Department of Agriculture FX Financial support for the study was provided through a specific cooperative agreement between University of California Riverside and United States Department of Agriculture. The sponsors had no involvement in the study design, analysis or interpretation of data. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The authors declare that they do not have any conflict of interest. NR 66 TC 6 Z9 6 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD NOV 12 PY 2015 VL 195 BP 124 EP 137 DI 10.1016/j.scienta.2015.09.004 PG 14 WC Horticulture SC Agriculture GA CW3HZ UT WOS:000364884100015 ER PT J AU Bergmann, GT Craine, JM Robeson, MS Fierer, N AF Bergmann, Gaddy T. Craine, Joseph M. Robeson, Michael S., II Fierer, Noah TI Seasonal Shifts in Diet and Gut Microbiota of the American Bison (Bison bison) SO PLOS ONE LA English DT Article ID PLANT-HERBIVORE INTERACTIONS; MIXED-GRASS PRAIRIE; TALLGRASS PRAIRIE; BACTERIAL COMMUNITIES; SPATIAL-DISTRIBUTION; GEOGRAPHIC-VARIATION; FORAGING ECOLOGY; HOLOCENE BISON; SPANISH GOATS; DAIRY-COWS AB North American bison (Bison bison) are becoming increasingly important to both grassland management and commercial ranching. However, a lack of quantitative data on their diet constrains conservation efforts and the ability to predict bison effects on grasslands. In particular, we know little about the seasonality of the bison diet, the degree to which bison supplement their diet with eudicots, and how changes in diet influence gut microbial communities, all of which play important roles in ungulate performance. To address these knowledge gaps, we quantified seasonal patterns in bison diet and gut microbial community composition for a bison herd in Kansas using DNA sequencing-based analyses of both chloroplast and microbial DNA contained in fecal matter. Across the 11 sampling dates that spanned 166 days, we found that diet shifted continuously over the growing season, allowing bison to take advantage of the seasonal availability of high-protein plant species. Bison consumed more woody shrubs in spring and fall than in summer, when forb and grass intake predominated. In examining gut microbiota, the bacterial phylum Tenericutes shifted significantly in relative abundance over the growing season. This work suggests that North American bison can continuously adjust their diet with a high reliance on non-grasses throughout the year. In addition, we find evidence for seasonal patterns in gut community composition that are likely driven by the observed dietary changes. C1 [Bergmann, Gaddy T.; Fierer, Noah] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Bergmann, Gaddy T.; Fierer, Noah] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA. [Craine, Joseph M.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Robeson, Michael S., II] Colorado State Univ, Fish & Wildlife Conservat Biol, Ft Collins, CO 80523 USA. [Robeson, Michael S., II] Natl Wildlife Res Ctr, USDA, APHIS, Ft Collins, CO USA. RP Bergmann, GT (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. EM Gaddy.Bergmann@colorado.edu RI Craine, Joseph/D-4569-2009; OI Craine, Joseph/0000-0001-6561-3244; Robeson, Michael/0000-0001-7119-6301 NR 108 TC 2 Z9 2 U1 13 U2 44 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 12 PY 2015 VL 10 IS 11 AR e0142409 DI 10.1371/journal.pone.0142409 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7UL UT WOS:000364480900032 PM 26562019 ER PT J AU Huff, ES Leahy, JE Hiebeler, D Weiskittel, AR Noblet, CL AF Huff, Emily Silver Leahy, Jessica E. Hiebeler, David Weiskittel, Aaron R. Noblet, Caroline L. TI An Agent-Based Model of Private Woodland Owner Management Behavior Using Social Interactions, Information Flow, and Peer-To-Peer Networks SO PLOS ONE LA English DT Article ID DECISION-MAKING; FOREST OWNERS; SIMULATION; LANDSCAPE; PROTOCOL; SYSTEMS; TRUST AB Privately owned woodlands are an important source of timber and ecosystem services in North America and worldwide. Impacts of management on these ecosystems and timber supply from these woodlands are difficult to estimate because complex behavioral theory informs the owner's management decisions. The decision-making environment consists of exogenous market factors, internal cognitive processes, and social interactions with fellow landowners, foresters, and other rural community members. This study seeks to understand how social interactions, information flow, and peer-to-peer networks influence timber harvesting behavior using an agent-based model. This theoretical model includes forested polygons in various states of 'harvest readiness' and three types of agents: forest landowners, foresters, and peer leaders (individuals trained in conservation who use peer-to-peer networking). Agent rules, interactions, and characteristics were parameterized with values from existing literature and an empirical survey of forest landowner attitudes, intentions, and demographics. The model demonstrates that as trust in foresters and peer leaders increases, the percentage of the forest that is harvested sustainably increases. Furthermore, peer leaders can serve to increase landowner trust in foresters. Model output and equations will inform forest policy and extension/outreach efforts. The model also serves as an important testing ground for new theories of landowner decision making and behavior. C1 [Huff, Emily Silver] US Forest Serv, USDA, No Res Stn, Amherst, MA USA. [Leahy, Jessica E.; Weiskittel, Aaron R.] Univ Maine, Sch Forest Resources, Orono, ME USA. [Hiebeler, David] Univ Maine, Dept Math & Stat, Orono, ME USA. [Noblet, Caroline L.] Univ Maine, Sch Econ, Orono, ME USA. RP Huff, ES (reprint author), US Forest Serv, USDA, No Res Stn, Amherst, MA USA. EM ehuff@fs.fed.us FU National Science Foundation (NSF) Sustainable Energy Pathways (SEP) [1230908]; NSF [EPS-0904155]; Maine Agricultural and Forest Experiment Station; Northeastern States Research Cooperative, USDA Forest Service; Sustainability Solutions Initiative FX This research was made possible by the National Science Foundation (NSF) Sustainable Energy Pathways (SEP) award #1230908 and as part of the Sustainability Solutions Initiative, supported by NSF award #EPS-0904155 to Maine EPSCoR at the University of Maine to JEL and ESH. This project was also supported by the Maine Agricultural and Forest Experiment Station and the Northeastern States Research Cooperative through funding made available by the USDA Forest Service to JEL and ESH. The conclusions and opinions in this paper are those of the authors and not the NSRC, the Forest Service, or the USDA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The authors would like to thank David B. Kittredge for providing internal validity checks of key model constructs. We also thank the Sustainability Solutions Initiative and Sustainable Energies Pathways team at the University of Maine for providing support and an intellectual environment to complete this work. We would also like to thank Tim Waring and Jared R. Stapp for early comments on the model. NR 55 TC 2 Z9 2 U1 2 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 12 PY 2015 VL 10 IS 11 AR e0142453 DI 10.1371/journal.pone.0142453 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7UL UT WOS:000364480900035 PM 26562429 ER PT J AU Fabrick, JA Unnithan, GC Yelich, AJ DeGain, B Masson, L Zhang, J Carriere, Y Tabashnik, BE AF Fabrick, Jeffrey A. Unnithan, Gopalan C. Yelich, Alex J. DeGain, Ben Masson, Luke Zhang, Jie Carriere, Yves Tabashnik, Bruce E. TI Multi-Toxin Resistance Enables Pink Bollworm Survival on Pyramided Bt Cotton SO SCIENTIFIC REPORTS LA English DT Article ID BACILLUS-THURINGIENSIS TOXINS; PECTINOPHORA-GOSSYPIELLA; INSECT RESISTANCE; TRICHOPLUSIA-NI; HELICOVERPA-ZEA; TRANSGENIC COTTON; DIAMONDBACK MOTH; CRY1AC TOXIN; LEPIDOPTERA; CROPS AB Transgenic crops producing Bacillus thuringiensis (Bt) proteins kill key insect pests, providing economic and environmental benefits. However, the evolution of pest resistance threatens the continued success of such Bt crops. To delay or counter resistance, transgenic plant "pyramids" producing two or more Bt proteins that kill the same pest have been adopted extensively. Field populations of the pink bollworm (Pectinophora gossypiell alpha) in the United States have remained susceptible to Bt toxins Cry1Ac and Cry2Ab, but field-evolved practical resistance to Bt cotton producing Cry1Ac has occurred widely in India. Here we used two rounds of laboratory selection to achieve 18,000-to 150,000-fold resistance to Cry2Ab in pink bollworm. Inheritance of resistance to Cry2Ab was recessive, autosomal, conferred primarily by one locus, and independent of Cry1Ac resistance. We created a strain with high resistance to both toxins by crossing the Cry2Ab-resistant strain with a Cry1Ac-resistant strain, followed by one selection with Cry2Ab. This multi-toxin resistant strain survived on field-collected Bt cotton bolls producing both toxins. The results here demonstrate the risk of evolution of resistance to pyramided Bt plants, particularly when toxins are deployed sequentially and refuges are scarce, as seen with Bt cotton and pink bollworm in India. C1 [Fabrick, Jeffrey A.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Unnithan, Gopalan C.; Yelich, Alex J.; DeGain, Ben; Carriere, Yves; Tabashnik, Bruce E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Masson, Luke] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada. [Zhang, Jie] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Haidian Distric, Peoples R China. RP Fabrick, JA (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM jeff.fabrick@ars.udsa.gov FU DuPont-Pioneer [58-3K95-4-1666]; Bayer CropScience; Dow AgroSciences; Monsanto; Syngenta FX This is a cooperative investigation between USDA ARS, the University of Arizona, and DuPont-Pioneer with J.A.F., B.E.T. and Y.C. receiving partial funding from DuPont-Pioneer to support this work (agreement #58-3K95-4-1666). J.A.F. is coauthor of a patent "Cadherin Receptor Peptide for Potentiating Bt Biopesticides" (patent numbers: US20090175974A1, US8354371, WO2009067487A2, WO2009067487A3). B.E.T. is a coauthor of a patent on modified Bt toxins, "Suppression of Resistance in Insects to Bacillus thuringiensis Cry Toxins, Using Toxins that do not Require the Cadherin Receptor" (patent numbers: CA2690188A1, CN101730712A, EP2184293A2, EP2184293A4, EP2184293B1, WO2008150150A2, WO2008150150A3). Bayer CropScience, Dow AgroSciences, Monsanto, and Syngenta did not provide funding to support this work, but may be affected financially by publication of this paper and have funded other work by some of the authors. NR 57 TC 4 Z9 4 U1 3 U2 43 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 12 PY 2015 VL 5 AR 16554 DI 10.1038/srep16554 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7OB UT WOS:000364462300001 PM 26559899 ER PT J AU Wear, DN Coulston, JW AF Wear, David N. Coulston, John W. TI From sink to source: Regional variation in US forest carbon futures SO Scientific Reports LA English DT Article ID UNITED-STATES; ACCUMULATION; PROJECTIONS; CLIMATE AB The sequestration of atmospheric carbon (C) in forests has partially offset C emissions in the United States (US) and might reduce overall costs of achieving emission targets, especially while transportation and energy sectors are transitioning to lower-carbon technologies. Using detailed forest inventory data for the conterminous US, we estimate forests' current net sequestration of atmospheric C to be 173 Tg yr(-1), offsetting 9.7% of C emissions from transportation and energy sources. Accounting for multiple driving variables, we project a gradual decline in the forest C emission sink over the next 25 years (to 112 Tg yr(-1)) with regional differences. Sequestration in eastern regions declines gradually while sequestration in the Rocky Mountain region declines rapidly and could become a source of atmospheric C due to disturbances such as fire and insect epidemics. C sequestration in the Pacific Coast region stabilizes as forests harvested in previous decades regrow. Scenarios simulating climate-induced productivity enhancement and afforestation policies increase sequestration rates, but would not fully offset declines from aging and forest disturbances. Separating C transfers associated with land use changes from sequestration clarifies forests' role in reducing net emissions and demonstrates that retention of forest land is crucial for protecting or enhancing sink strength. C1 [Wear, David N.] N Carolina State Univ, US Forest Serv, USDA, Raleigh, NC 27695 USA. [Coulston, John W.] US Forest Serv, USDA, Knoxville, TN 37919 USA. RP Wear, DN (reprint author), N Carolina State Univ, US Forest Serv, USDA, POB 8008, Raleigh, NC 27695 USA. EM dwear@fs.fed.us NR 32 TC 1 Z9 1 U1 4 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD NOV 12 PY 2015 VL 5 AR 16518 DI 10.1038/srep16518 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7NX UT WOS:000364461900002 PM 26558439 ER PT J AU Li, XL Liu, C Ip, BC Hu, KQ Smith, DE Greenberg, AS Wang, XD AF Li, Xinli Liu, Chun Ip, Blanche C. Hu, Kang-Quan Smith, Donald E. Greenberg, Andrew S. Wang, Xiang-Dong TI Tumor progression locus 2 ablation suppressed hepatocellular carcinoma development by inhibiting hepatic inflammation and steatosis in mice SO JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH LA English DT Article DE TPL2; HCC; Tumorigenesis; Inflammation; Steatosis ID TRANSCRIPTION FACTOR XBP1; FATTY LIVER-DISEASE; SERINE/THREONINE KINASE; TUMORIGENESIS; EXPRESSION; STRESS; CANCER; PHOSPHORYLATION; OBESITY; CELLS AB Background: Tumor progression locus 2 (TPL2), a serine-threonine kinase, functions as a critical regulator of inflammatory pathways and mediates oncogenic events. The potential role of Tpl2 in nonalcoholic fatty liver disease (NAFLD) associated hepatocellular carcinoma (HCC) development remains unknown. Methods: Both wild-type and Tpl2 knockout male mice were initiated by a hepatic carcinogen (diethylnitrosamine, i.p. with a single dose of 25 mg.kg(-1)) at 2 weeks of age, and then were given the high carbohydrate diet feeding to induce hepatic steatosis, inflammation, adenoma and HCC for 24 weeks. Results: Tpl2 knockout mice had significantly lower incidences of liver tumor and developed hepatocellular adenoma only, which is contrast to wild-type mice where they all developed HCC. Tpl2 knockout mice had significantly down-regulated phosphorylation of JNK and ERK, and levels of mRNA expression of pro-inflammatory cytokines (Il-1 beta, Il-18, Mcp-1 and Nalp3), which correlated with the reduced incidence and number of hepatic inflammatory foci. Furthermore, Tpl2 ablation resulted in decreased hepatic steatosis and expression of de novo lipogenesis related markers (ACC, SCD1, SREBP1C and AKT phosphorylation), as well as reduction of endoplasmic reticulum stress biomarkers PERK and eIF-2a. Conclusion: The study revealed for the first time that Tpl2 plays a significant role in promoting HCC development by its pro-inflammatory effect, which suggested that Tpl2 could be a molecular target for HCC prevention. C1 [Li, Xinli; Liu, Chun; Ip, Blanche C.; Hu, Kang-Quan; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Smith, Donald E.] Comparat Biol Unit, Boston, MA USA. [Greenberg, Andrew S.] Tufts Univ, Obes & Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Li, Xinli] Soochow Univ, Coll Med, Sch Publ Hlth, Suzhou 215123, Jiangsu, Peoples R China. RP Wang, XD (reprint author), Tufts Univ, Nutr & Canc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu OI IP, Blanche C/0000-0003-0063-5535 FU State Scholarship Fund by The China Scholarship Council [2011832272]; NIH [CA176256]; USDA/ARS grant [58-1950-0014]; HNRCA Cancer Cluster funding FX The author thanks Dr. Camilla P. Stice, and the HNRCA Cancer Cluster members for their contributions to this study. Dr. Xinli Li was supported by a scholarship (File NO. 2011832272) from the State Scholarship Fund by The China Scholarship Council.; Financial support was provided by the NIH grant (CA176256), USDA/ARS grant (58-1950-0014), and HNRCA Cancer Cluster funding. NR 42 TC 6 Z9 6 U1 0 U2 0 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-9966 J9 J EXP CLIN CANC RES JI J. Exp. Clin. Cancer Res. PD NOV 11 PY 2015 VL 34 AR 138 DI 10.1186/s13046-015-0254-2 PG 8 WC Oncology SC Oncology GA CY6BC UT WOS:000366491100001 PM 26560698 ER PT J AU Hu, Y Wu, QY Sprague, SA Park, J Oh, M Rajashekar, CB Koiwa, H Nakata, PA Cheng, NH Hirschi, KD White, FF Park, S AF Hu, Ying Wu, Qingyu Sprague, Stuart A. Park, Jungeun Oh, Myungmin Rajashekar, C. B. Koiwa, Hisashi Nakata, Paul A. Cheng, Ninghui Hirschi, Kendal D. White, Frank F. Park, Sunghun TI Tomato expressing Arabidopsis glutaredoxin gene AtGRXS17 confers tolerance to chilling stress via modulating cold responsive components SO HORTICULTURE RESEARCH LA English DT Article ID SHOOT MERISTEM SIZE; RICE ORYZA-SATIVA; OXIDATIVE STRESS; ABIOTIC STRESS; TRANSCRIPTION FACTOR; FREEZING TOLERANCE; PHOTOSYNTHETIC ORGANISMS; MONOTHIOL GLUTAREDOXIN; NUCLEAR TRANSLOCATION; ANTIOXIDANT DEFENSE AB Chilling stress is a production constraint of tomato, a tropical origin, chilling-sensitive horticultural crop. The development of chilling tolerant tomato thus has significant potential to impact tomato production. Glutaredoxins (GRXs) are ubiquitous oxidoreductases, which utilize the reducing power of glutathione to reduce disulfide bonds of substrate proteins and maintain cellular redox homeostasis. Here, we report that tomato expressing Arabidopsis GRX gene AtGRXS17 conferred tolerance to chilling stress without adverse effects on growth and development. AtGRXS17-expressing tomato plants displayed lower ion leakage, higher maximal photochemical efficiency of photosystem II (Fv/Fm) and increased accumulation of soluble sugar compared with wild-type plants after the chilling stress challenge. Furthermore, chilling tolerance was correlated with increased antioxidant enzyme activities and reduced H2O2 accumulation. At the same time, temporal expression patterns of the endogenous C-repeat/DRE-binding factor 1 (SlCBF1) and CBF mediated-cold regulated genes were not altered in AtGRXS17-expressing plants when compared with wild-type plants, and proline concentrations remained unchanged relative to wild-type plants under chilling stress. Green fluorescent protein -AtGRXS17 fusion proteins, which were initially localized in the cytoplasm, migrated into the nucleus during chilling stress, reflecting a possible role of AtGRXS17 in nuclear signaling of chilling stress responses. Together, our findings demonstrate that genetically engineered tomato plants expressing AtGRXS17 can enhance chilling tolerance and suggest a genetic engineering strategy to improve chilling tolerance without yield penalty across different crop species. C1 [Hu, Ying; Wu, Qingyu; Sprague, Stuart A.; Park, Jungeun; Oh, Myungmin; Rajashekar, C. B.; Park, Sunghun] Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. [Koiwa, Hisashi] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Nakata, Paul A.; Cheng, Ninghui; Hirschi, Kendal D.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [White, Frank F.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Park, S (reprint author), Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. EM shpark@ksu.edu RI Wu, Qingyu/E-4636-2017 OI Wu, Qingyu/0000-0003-3064-2445 NR 84 TC 4 Z9 5 U1 3 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD NOV 11 PY 2015 VL 2 AR 15051 DI 10.1038/hortres.2015.51 PG 11 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA CY1BZ UT WOS:000366143000001 PM 26623076 ER PT J AU Agrafioti, P Athanassiou, CG Vassilakos, TN Vlontzos, G Arthur, FH AF Agrafioti, Paraskevi Athanassiou, Christos G. Vassilakos, Thomas N. Vlontzos, George Arthur, Frank H. TI Using a Lethality Index to Assess Susceptibility of Tribolium confusum and Oryzaephilus surinamensis to Insecticides SO PLOS ONE LA English DT Article ID STORED-PRODUCT INSECTS; RESIDUAL EFFICACY; DIFFERENT SURFACES; BETA-CYFLUTHRIN; SHORT EXPOSURES; FOOD SOURCE; CONCRETE; DELTAMETHRIN; CHLORFENAPYR; COLEOPTERA AB We evaluated knockdown caused by four insecticides: alpha-cypermethrin, chlorfenapyr, pirimiphos-methyl and fipronil against adults of Tribolium confusum Jacquelin Duval, the confused flour beetle and Oryzaephilus surinamensis (L.), the sawtoothed grain beetle. Bioassays were conducted on concrete and metal surfaces. Adults of the tested species were exposed on both surfaces treated with the above insecticides at two doses (low and high). Knockdown assessment was done after 15, 30 and 60 min of adult exposure in the treated surfaces. Also, after 1, 3, 5, 7 and 14 d of exposure, a lethality index was calculated with an equation resulting to values from 0 to 100, where 100 indicated complete mortality and 0 complete survival. We also developed a lethality index by ranking each adult on each surface from 0 to 4, 0: adults moved normally, 1: adults were knocked down, but were able to walk for short intervals, 2: adults were knocked down and unable to walk, but with visible movement of antennae etc., 3: adults were knocked down, with very minimal movement of the tarsi and the antennae and 4: adults were dead (no movement). Knockdown of adults immediately after exposure (15-60 min) was higher for pirimiphos-methyl followed by alpha-cypermethrin, for both dose rates tested and species, but only on the metal surface. The lethality index was nearly 100 for all insecticides after 5d of exposure for O. surinamensis, while for T. confusum the adult lethality index was considerably lower for alpha-cypermethrin, suggesting that that recovery from knockdown occurred. Chlorfenapyr was the only insecticide that was more effective on concrete than on metal, while the reverse was noted for the other three insecticides. These results show that knockdown has different levels, which can be used as indicators of insect mortality or recovery. C1 [Agrafioti, Paraskevi; Athanassiou, Christos G.; Vassilakos, Thomas N.; Vlontzos, George] Univ Thessaly, Dept Agr Crop Prod & Rural Environm, Volos, Greece. [Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Athanassiou, CG (reprint author), Univ Thessaly, Dept Agr Crop Prod & Rural Environm, Lab Entomol & Agr Zool, Volos, Greece. EM athanassiou@agr.uth.gr NR 30 TC 0 Z9 0 U1 3 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 11 PY 2015 VL 10 IS 11 AR e0142044 DI 10.1371/journal.pone.0142044 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7DR UT WOS:000364433100051 PM 26560316 ER PT J AU Makkar, S Liyanage, R Kannan, L Packialakshmi, B Lay, JO Rath, NC AF Makkar, Sarbjeet Liyanage, Rohana Kannan, Lakshmi Packialakshmi, Balamurugan Lay, Jack O., Jr. Rath, Narayan C. TI Chicken Egg Shell Membrane Associated Proteins and Peptides SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE egg shell; membrane; mass spectrometry; proteins ID PLASMODIUM-FALCIPARUM; PROTEOMIC ANALYSIS; MATRIX PROTEINS; CALCIFIED LAYER; WHITE PROTEOME; ORGANIC MATRIX; HEN EGG; EGGSHELL; IDENTIFICATION; FAMILY AB Egg shells are poultry industry byproducts with potential for use in various biological and agricultural applications. We have been interested in the membranes underlying the calcareous shell as a feed supplement, which showed potential to improve immunity and performance of post hatch poultry. Therefore, to determine their protein and peptide profiles, we extracted the egg shell membranes (ESM) from fresh unfertilized eggs with methanol and guanidine hydrochloride (GdHCl) to obtain soluble proteins for analysis by mass spectrometry. The methanol extract was subjected to matrix-assisted laser desorption ionization (MALDI), electrospray ionization (ESI), high-performance reverse phase liquid chromatographic separation (HPLC), and tandem mass spectrometry (MS/MS) to determine its peptide and protein profiles. The GdHCl extract was subjected to ESI-HPLC-MS/MS following trypsin digestion of reduced/alkylated proteins. Nine proteins from the methanol extract and >275 proteins from the GdHCl extract were tentatively identified. The results suggested the presence of several abundant proteins from egg whites, such as ovoalbumin, ovotransferrin, and lysozyme as well as many others associated with antimicrobial, biomechanical, cytoskeletal organizational, cell signaling, and enzyme activities. Collagens, keratin, agrin, and laminin were some of the structural proteins present in the ESM. The methanol-soluble fraction contained several clusterin peptides and defensins, particularly, two isoforms of gallin. The ratios of the two isoforms of gallin differed between the membranes obtained from brown and white eggs. The high abundance of several antimicrobial, immunomodulatory, and other bioactive proteins in the ESM along with its potential to entrap various microbes and antigens may make it a suitable vehicle for oral immunization of post hatch poultry and improve their disease resistance. C1 [Makkar, Sarbjeet; Kannan, Lakshmi; Packialakshmi, Balamurugan; Rath, Narayan C.] Univ Arkansas, Poultry Prod & Prod Safety Res Unit, ARS, USDA, Fayetteville, AR 72701 USA. [Makkar, Sarbjeet] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Liyanage, Rohana; Lay, Jack O., Jr.] Univ Arkansas, State Wide Mass Spectrometry Facil, Fayetteville, AR 72701 USA. [Kannan, Lakshmi; Packialakshmi, Balamurugan] Univ Arkansas, Cell & Mol Biol Program, Fayetteville, AR 72701 USA. RP Rath, NC (reprint author), Univ Arkansas, USDA ARS, Poultry Sci Ctr, O-307, Fayetteville, AR 72701 USA. EM Narayan.rath@ars.usda.gov RI Lay, Jackson/G-1007-2011 OI Lay, Jackson/0000-0003-3789-2527 FU Arkansas Bioscience Institute grant; NIH Grant [P30 GM103450] FX This work was carried as a USDA/ARS project and was supported partly by an Arkansas Bioscience Institute grant to R.L. and by the State Wide mass spectrometry facility, supported by NIH Grant P30 GM103450 to the University of Arkansas. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable. NR 51 TC 5 Z9 5 U1 18 U2 67 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 11 PY 2015 VL 63 IS 44 BP 9888 EP 9898 DI 10.1021/acs.jafc.5b04266 PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CW1CO UT WOS:000364727400026 PM 26485361 ER PT J AU Millar, CI Westfall, RD Evenden, A Holmquist, JG Schmidt-Gengenbach, J Franklin, RS Nachlinger, J Delany, DL AF Millar, Constance I. Westfall, Robert D. Evenden, Angela Holmquist, Jeffrey G. Schmidt-Gengenbach, Jutta Franklin, Rebecca S. Nachlinger, Jan Delany, Diane L. TI Potential climatic refugia in semi-arid, temperate mountains: Plant and arthropod assemblages associated with rock glaciers, talus slopes, and their forefield wetlands, Sierra Nevada, California, USA SO QUATERNARY INTERNATIONAL LA English DT Article; Proceedings Paper CT 26th Pacific Climate Workshop (PACLIM) CY MAR 03-06, 2013 CL Pacific Grove, CA ID DEBRIS-COVERED GLACIERS; PIKA OCHOTONA-PRINCEPS; COLORADO FRONT RANGE; THERMAL REGIME; PERIGLACIAL LANDFORMS; BLOCK FIELDS; ACTIVE LAYER; STONY DEBRIS; DIVERSITY; LANDSCAPE AB Unique thermal and hydrologic regimes of rock-glacier and periglacial talus environments support little-studied mountain ecosystems. We report the first studies of vascular plant and arthropod diversity for these habitats in the central Sierra Nevada, California, USA. Surfaces of active rock glaciers develop scattered islands of soil that provide habitat for vegetation. Total plant cover relative to the entire surface area of two rock glaciers was 0.2-1.7%. Vascular plant diversity was high relative to reference sites, with 16-28 species observed on 0.1-1 ha total area of soils patches on each rock glacier. Species had upland-and cold-adapted traits, and were primarily perennial herbs and subshrubs. Complex wetland environments in the forefield of two rock glaciers and two talus slopes supported a high diversity of vascular plants, with sedges and graminoid taxa abundant as well as other cold-environment, wetland-adapted, perennial herbaceous species. Talus forefields were small (mean, 0.6 ha) yet supported 65 vascular plant species each; larger rock-glacier forefields (mean, 11 ha) supported 48-84 species each, with diversity greater at the larger site. Relative to reference sites, taxonomic diversity on the wetlands associated with periglacial landforms overlapped that on reference wetlands. Arthropod diversity at the same four rock-glacier and talus wetlands was significantly higher than at reference wetlands, with overall abundance three times greater on the rock-associated wetlands. Forty-seven of the 60 arthropod families collected were more abundant in rock glacier and talus wetlands than in reference meadows. On average, 17 families (26 morphospecies) were observed on the talus wetlands and 45 families (69 morphospecies) on rock-glacier wetlands. Cicadellid leaf-hoppers and aphids were dominant on the rock-associated wetlands whereas chloropid flies were most abundant on reference sites. Given the known thermal and hydrologic capacity of rock-glacier and talus environments to resist warming, these distinct ecosystems might become increasingly important as mountain refugia for a diversity of biota in the future. (C) 2013 Elsevier Ltd and INQUA. All rights reserved. C1 [Millar, Constance I.; Westfall, Robert D.; Delany, Diane L.] US Forest Serv, USDA, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. [Evenden, Angela] Univ Calif Berkeley, USDI, Calif Cooperat Ecosyst Studies Unit, Berkeley, CA 94720 USA. [Holmquist, Jeffrey G.; Schmidt-Gengenbach, Jutta] Univ Calif Los Angeles, Inst Environm & Sustainabil, White Mt Res Ctr, Bishop, CA 93514 USA. [Franklin, Rebecca S.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Nachlinger, Jan] Nevada Native Plant Soc, Reno, NV 89507 USA. RP Millar, CI (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. EM cmillar@fs.fed.us; bwestfall@fs.fed.us; angela_evenden@nps.gov; jholmquist@ucla.edu; juttasg@ucla.edu; rfranklin@cocc.edu; jangr8basn@charter.net; ddelany@fs.fed.us FU Sierra Nevada Network Inventory and Monitoring Program of the National Park Service through the Great Basin Cooperative Ecosystems Studies Unit [J8R07070006] FX We thank Elizabeth Wenk for help in identifying plant species on the Barney and Gibb rock-glacier surfaces, Jason Love and Jennifer Jones for field assistance in arthropod sampling, and Marie French, Jean Dillingham, Derham Giuliani, and Peter Norquist for sorting faunal samples. The arthropod reference sites were sampled with support from the Sierra Nevada Network Inventory and Monitoring Program of the National Park Service (J8R07070006), which was funded through the Great Basin Cooperative Ecosystems Studies Unit with the assistance of Angela Evenden. NR 97 TC 6 Z9 7 U1 4 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1040-6182 EI 1873-4553 J9 QUATERN INT JI Quat. Int. PD NOV 11 PY 2015 VL 387 BP 106 EP 121 DI 10.1016/j.quaint.2013.11.003 PG 16 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CV8LR UT WOS:000364539500011 ER PT J AU Mou, ZY Hyde, TM Lipska, BK Martinowich, K Wei, P Ong, CJ Hunter, LA Palaguachi, GI Morgun, E Teng, R Lai, C Condarco, TA Demidowich, AP Krause, AJ Marshall, LJ Haack, K Voruganti, VS Cole, SA Butte, NF Comuzzie, AG Nalls, MA Zonderman, AB Singleton, AB Evans, MK Martin, B Maudsley, S Tsao, JW Kleinman, JE Yanovski, JA Han, JC AF Mou, Zongyang Hyde, Thomas M. Lipska, Barbara K. Martinowich, Keri Wei, Peter Ong, Chiew-Jen Hunter, Lindsay A. Palaguachi, Gladys I. Morgun, Eva Teng, Rujia Lai, Chen Condarco, Tania A. Demidowich, Andrew P. Krause, Amanda J. Marshall, Leslie J. Haack, Karin Voruganti, V. Saroja Cole, Shelley A. Butte, Nancy F. Comuzzie, Anthony G. Nalls, Michael A. Zonderman, Alan B. Singleton, Andrew B. Evans, Michele K. Martin, Bronwen Maudsley, Stuart Tsao, Jack W. Kleinman, Joel E. Yanovski, Jack A. Han, Joan C. TI Human Obesity Associated with an Intronic SNP in the Brain-Derived Neurotrophic Factor Locus SO CELL REPORTS LA English DT Article ID HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; BINDING; TRANSCRIPTION; EXPRESSION; GENETICS; COMPLEX; BDNF AB Brain-derived neurotrophic factor (BDNF) plays a key role in energy balance. In population studies, SNPs of the BDNF locus have been linked to obesity, but the mechanism by which these variants cause weight gain is unknown. Here, we examined human hypothalamic BDNF expression in association with 44 BDNF SNPs. We observed that the minor C allele of rs12291063 is associated with lower human ventromedial hypothalamic BDNF expression (p < 0.001) and greater adiposity in both adult and pediatric cohorts (p values < 0.05). We further demonstrated that the major T allele for rs12291063 possesses a binding capacity for the transcriptional regulator, heterogeneous nuclear ribonucleoprotein D0B, knockdown of which disrupts transactivation by the T allele. Binding and transactivation functions are both disrupted by substituting C for T. These findings provide a rationale for BDNF augmentation as a targeted treatment for obesity in individuals who have the rs12291063 CC genotype. C1 [Mou, Zongyang; Wei, Peter; Ong, Chiew-Jen; Hunter, Lindsay A.; Palaguachi, Gladys I.; Morgun, Eva; Teng, Rujia; Lai, Chen; Han, Joan C.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Metab & Neuroendocrinol, NIH, Bethesda, MD 20892 USA. [Mou, Zongyang; Morgun, Eva; Condarco, Tania A.; Demidowich, Andrew P.; Krause, Amanda J.; Tsao, Jack W.; Yanovski, Jack A.; Han, Joan C.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Growth & Obes, NIH, Bethesda, MD 20892 USA. [Hyde, Thomas M.; Martinowich, Keri; Kleinman, Joel E.] Johns Hopkins Univ, Sch Med, Lieber Inst Brain Dev, Baltimore, MD 21205 USA. [Hyde, Thomas M.; Martinowich, Keri; Kleinman, Joel E.] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA. [Hyde, Thomas M.; Kleinman, Joel E.] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA. [Lipska, Barbara K.] NIMH, Human Brain Collect Core, NIH, Bethesda, MD 20892 USA. [Wei, Peter; Ong, Chiew-Jen; Hunter, Lindsay A.; Palaguachi, Gladys I.] Uniformed Serv Univ Hlth Sci, Dept Neurol, Bethesda, MD 20814 USA. [Wei, Peter; Ong, Chiew-Jen; Hunter, Lindsay A.; Palaguachi, Gladys I.; Lai, Chen] Uniformed Serv Univ Hlth Sci, Dept Phys Med & Rehabil, Bethesda, MD 20814 USA. [Marshall, Leslie J.] NIAID, Preclin Microbicide & Prevent Res Branch, NIH, Bethesda, MD 20892 USA. [Haack, Karin; Voruganti, V. Saroja; Cole, Shelley A.; Comuzzie, Anthony G.] Texas Biomed Res Inst, Dept Genet, San Antonio, TX 78245 USA. [Haack, Karin; Voruganti, V. Saroja; Cole, Shelley A.; Comuzzie, Anthony G.] Southwest Natl Primate Res Ctr, San Antonio, TX 78245 USA. [Voruganti, V. Saroja] Univ N Carolina, Dept Nutr, Kannapolis, NC 28081 USA. [Voruganti, V. Saroja] Univ N Carolina, UNC Nutr Res Inst, Kannapolis, NC 28081 USA. [Butte, Nancy F.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Nalls, Michael A.; Singleton, Andrew B.] NIA, Mol Genet Sect, Bethesda, MD 20892 USA. [Zonderman, Alan B.] NIA, Behav Epidemiol Sect, Baltimore, MD 21224 USA. [Evans, Michele K.] NIA, Hlth Dispar Res Sect, Baltimore, MD 21224 USA. [Martin, Bronwen] NIA, Metab Unit, Baltimore, MD 21224 USA. [Maudsley, Stuart] NIA, Receptor Pharmacol Unit, Baltimore, MD 21224 USA. [Maudsley, Stuart] Univ Antwerp, Translat Neurobiol Grp, VIB Dept Mol Genet, B-2610 Antwerp, Belgium. [Maudsley, Stuart] Univ Antwerp, Lab Neurogenet, Inst Born Bunge, B-2610 Antwerp, Belgium. [Tsao, Jack W.] Univ Tennessee, Hlth Sci Ctr, Dept Neurol, Memphis, TN 38163 USA. [Han, Joan C.] Univ Tennessee, Hlth Sci Ctr, Dept Pediat, Memphis, TN 38163 USA. [Han, Joan C.] Le Bonheur Childrens Hosp, Childrens Fdn Res Inst, Memphis, TN 38103 USA. RP Han, JC (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Metab & Neuroendocrinol, NIH, Bethesda, MD 20892 USA. EM jhan14@uthsc.edu RI Singleton, Andrew/C-3010-2009; Martinowich, Keri/F-9841-2012; Lipska, Barbara/E-4569-2017; OI Zonderman, Alan B/0000-0002-6523-4778 FU Intramural Research Program of NICHD; NIH [R01 DK59264, R01 DK080457]; NIMH; National Institute of Minority Health and Health Disparities; National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); NIH Division of Nutrition Research Coordination; National Human Genome Research Institute (NHGRI); CALiCo [U01HG004803]; EAGLE [U01HG004798]; MEC [U01HG004802]; WHI [U01HG004790]; Coordinating Center [U01HG004801] FX This research was supported by the Intramural Research Program of NICHD and NIMH with supplemental funding from the NIH Bench to Bedside Program to J.C.H. and the National Institute of Minority Health and Health Disparities to J.A.Y. The Viva la Familia studies were supported by the NIH (R01 DK59264 and R01 DK080457 to N.F.B.). A.J.K. is supported by the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) and the NIH Division of Nutrition Research Coordination. The PAGE program is funded by National Human Genome Research Institute (NHGRI), supported by U01HG004803 (CALiCo), U01HG004798 (EAGLE), U01HG004802 (MEC), U01HG004790 (WHI), and U01HG004801 (Coordinating Center), and their respective NHGRI American Recovery and Reinvestment Act (ARRA) supplements. We thank Ms. Amy Deep-Soboslay and Drs. Lewellyn B. Bigelow and Mary M. Herman for their contributions in assembling the postmortem human brain cohorts for study, Dr. Amanda Preston for editorial assistance, and the families of the decedents for their generous donation of tissue. J.A.Y. is a Commissioned Officer of the United States Public Health Service. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Public Health Service, Department of the Navy, Department of Defense, or Department of Health and Human Services. NR 20 TC 8 Z9 8 U1 0 U2 3 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 2211-1247 J9 CELL REP JI Cell Reports PD NOV 10 PY 2015 VL 13 IS 6 BP 1073 EP 1080 DI 10.1016/j.celrep.2015.09.065 PG 8 WC Cell Biology SC Cell Biology GA CW4UJ UT WOS:000364990600001 PM 26526993 ER PT J AU Bradford, SA Torkzaban, S AF Bradford, Scott A. Torkzaban, Saeed TI Determining Parameters and Mechanisms of Colloid Retention and Release in Porous Media SO LANGMUIR LA English DT Article ID UNFAVORABLE SURFACE INTERACTIONS; CORRELATION EQUATION; MICROBIAL TRANSPORT; FILTER COEFFICIENT; PRIMARY-MINIMUM; DEPOSITION; PARTICLES; SUBSURFACE; HETEROGENEITY; ATTACHMENT AB A modeling framework is presented to determine fundamental parameters and controlling mechanisms of colloid (microbes, clays, and nanoparticles) retention and release on surfaces of porous media that exhibit wide distributions of nanoscale chemical heterogeneity, nano- to microscale roughness, and pore water velocity. Primary and/or secondary minimum interactions in the zone of electrostatic influence were determined over the heterogeneous solid surface. The Maxwellian kinetic energy model was subsequently employed to determine the probability of immobilization and diffusive release of colloids from each of these minima. In addition, a balance of applied hydrodynamic and resisting adhesive torques was conducted to determine locations of immobilization and hydrodynamic release in the presence of spatially variable water flow and microscopic roughness. Locations for retention had to satisfy both energy and torque balance conditions for immobilization, whereas release could occur either due to diffusion or hydrodynamics Summation of energy and torque balance results over the elementary surface area of the porous medium provided estimates for colloid retention and release parameters that are critical to predicting environmental fate, including the sticking and release efficiencies and the maximum concentration of retained colloids on the solid phase. Nanoscale roughness and chemical heterogeneity produced localized primary minimum interactions that controlled long-term retention, even when mean chemical conditions were unfavorable. Microscopic roughness played a dominant role in colloid retention under low ionic strength and high hydrodynamic conditions, especially for larger colloids. C1 [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Torkzaban, Saeed] CSIRO Land & Water, Glen Osmond, SA 5064, Australia. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. EM scott.bradford@ars.usda.gov RI Torkzaban, Saeed/G-7377-2013 OI Torkzaban, Saeed/0000-0002-5146-9461 FU USDA, ARS [NP 214] FX This research was supported by the USDA, ARS, NP 214. The USDA is an equal opportunity provider and employer. NR 60 TC 13 Z9 13 U1 10 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0743-7463 J9 LANGMUIR JI Langmuir PD NOV 10 PY 2015 VL 31 IS 44 BP 12096 EP 12105 DI 10.1021/acs.langmuir.5b03080 PG 10 WC Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary SC Chemistry; Materials Science GA CV9NY UT WOS:000364615100017 PM 26484563 ER PT J AU Hau, SJ Sun, JS Davies, PR Frana, TS Nicholson, TL AF Hau, Samantha J. Sun, Jisun Davies, Peter R. Frana, Timothy S. Nicholson, Tracy L. TI Comparative Prevalence of Immune Evasion Complex Genes Associated with beta-Hemolysin Converting Bacteriophages in MRSA ST5 Isolates from Swine, Swine Facilities, Humans with Swine Contact, and Humans with No Swine Contact SO PLoS One LA English DT Article ID RESISTANT STAPHYLOCOCCUS-AUREUS; CHEMOTAXIS INHIBITORY PROTEIN; UNITED-STATES; MOLECULAR CHARACTERIZATION; COLONIZATION; STRAINS; HOST; PIGS; STAPHYLOKINASE; EPIDEMIOLOGY AB Livestock associated methicillin-resistant Staphylococcus aureus (LA-MRSA) draws concern from the public health community because in some countries these organisms may represent the largest reservoir of MRSA outside hospital settings. Recent studies indicate LA-MRSA strains from swine are more genetically diverse than the first reported sequence type ST398. In the US, a diverse population of LA-MRSA is found including organisms of the ST398, ST9, and ST5 lineages. Occurrence of ST5 MRSA in swine is of particular concern since ST5 is among the most prevalent lineages causing clinical infections in humans. The prominence of ST5 in clinical disease is believed to result from acquisition of bacterio-phages containing virulence or host-adapted genes including the immune-evasion cluster (IEC) genes carried by beta-hemolysin converting bacteriophages, whose absence in LAMRSA ST398 is thought to contribute to reduced rates of human infection and transmission associated with this lineage. The goal of this study was to investigate the prevalence of IEC genes associated with beta-hemolysin converting bacteriophages in MRSA ST5 isolates obtained from agricultural sources, including swine, swine facilities, and humans with shortor long-term swine exposure. To gain a broader perspective, the prevalence of these genes in LA-MRSA ST5 strains was compared to the prevalence in clinical MRSA ST5 strains from humans with no known exposure to swine. IEC genes were not present in any of the tested MRSA ST5 strains from agricultural sources and the beta-hemolysin gene was intact in these strains, indicating the bacteriophage's absence. In contrast, the prevalence of the beta-hemolysin converting bacteriophage in MRSA ST5 strains from humans with no exposure to swine was 90.4%. The absence of beta-hemolysin converting bacteriophage in LA-MRSA ST5 isolates is consistent with previous reports evaluating ST398 strains and provides genetic evidence indicating LA-MRSA ST5 isolates may harbor a reduced capacity to cause severe disease in immunocompetent humans. C1 [Hau, Samantha J.; Frana, Timothy S.] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Sun, Jisun; Davies, Peter R.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. [Nicholson, Tracy L.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Nicholson, TL (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM tracy.nicholson@ars.usda.gov FU National Pork Board [14-124]; United States Department of Agriculture, Agriculture Research Service [5030-32000-109-00D]; Centers for Disease Control and Prevention [U54OH010170]; Lora and Russ Talbot Graduate Fellowship FX Funding, wholly or in part, was provided by The National Pork Board (#14-124) and by the United States Department of Agriculture, Agriculture Research Service (Project#5030-32000-109-00D). Isolates from swine veterinarians were obtained with funding under cooperative agreement award U54OH010170 (Upper Midwest Agricultural Safety and Health Center) from the Centers for Disease Control and Prevention. SJH is supported by the Lora and Russ Talbot Graduate Fellowship. The funders of the work did not influence study design, data collection and analysis, decision to publish, and preparation of the manuscript. NR 43 TC 3 Z9 3 U1 1 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 10 PY 2015 VL 10 IS 11 AR e0142832 DI 10.1371/journal.pone.0142832 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7CT UT WOS:000364430700178 PM 26554919 ER PT J AU Torres, GL Cooper, WR Horton, DR Swisher, KD Garczynski, SF Munyaneza, JE Barcenas, NM AF Torres, Glenda L. Cooper, W. Rodney Horton, David R. Swisher, Kylie D. Garczynski, Stephen F. Munyaneza, Joseph E. Barcenas, Nina M. TI Horizontal Transmission of "Candidatus Liberibacter solanacearum" by Bactericera cockerelli (Hemiptera: Triozidae) on Convolvulus and Ipomoea (Solanales: Convolvulaceae) SO PLOS ONE LA English DT Article ID UNITED-STATES; PCR ASSAY; POTATO; TOMATO; SULC; HAPLOTYPES; DISEASE; PLANTS; TIME AB "Candidatus Liberibacter solanacearum" (Proteobacteria) is an important pathogen of solanaceous crops (Solanales: Solanaceae) in North America and New Zealand, and is the putative causal agent of zebra chip disease of potato. This phloem-limited pathogen is transmitted to potato and other solanaceous plants by the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae). While some plants in the Convolvulaceae (Solanales) are also known hosts for B. cockerelli, previous efforts to detect Liberibacter in Convolvulaceae have been unsuccessful. Moreover, studies to determine whether Liberibacter can be acquired from these plants by B. cockerelli are lacking. The goal of this study was to determine whether horizontal transmission of Liberibacter occurs among potato psyllids on two species of Convolvulaceae, sweet potato (Ipomoea batatas) and field bindweed (Convolvulus arvensis), which grows abundantly in potato growing regions of the United States. Results indicated that uninfected psyllids acquired Liberibacter from both I. batatas and C. arvensis if infected psyllids were present on plants concurrently with the uninfected psyllids. Uninfected psyllids did not acquire Liberibacter from plants if the infected psyllids were removed from the plants before the uninfected psyllids were allowed access. In contrast with previous reports, PCR did detect the presence of Liberibacter DNA in some plants. However, visible amplicons were faint and did not correspond with acquisition of the pathogen by uninfected psyllids. None of the plants exhibited disease symptoms. Results indicate that horizontal transmission of Liberibacter among potato psyllids can occur on Convolvulaceae, and that the association between Liberibacter and Convolvulaceae merits additional attention. C1 [Torres, Glenda L.; Barcenas, Nina M.] Heritage Univ, Dept Nat Sci, Toppenish, WA 98948 USA. [Cooper, W. Rodney; Horton, David R.; Swisher, Kylie D.; Garczynski, Stephen F.; Munyaneza, Joseph E.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Cooper, WR (reprint author), ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov FU National Science Foundation Research Experience for Undergraduates Award [DBI-1156603]; NSF-REU [DBI-1156603] FX Glenda Torres was supported by National Science Foundation Research Experience for Undergraduates Award DBI-1156603 to NMB. (http://www.nsf.gov/funding/pgm_summ.jsp?pims_id=5517 NMB). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; Heather Headrick and Pauline Anderson provided technical assistance. Glenda Torres was supported by NSF-REU Award DBI-1156603 to Heritage University (PI Nina Barcenas). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 23 TC 0 Z9 0 U1 2 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 10 PY 2015 VL 10 IS 11 AR e0142734 DI 10.1371/journal.pone.0142734 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV7CT UT WOS:000364430700162 PM 26555359 ER PT J AU Simpson, KA Mavros, Y Kay, S Meiklejohn, J de Vos, N Wang, Y Guo, QY Zhao, RR Climstein, M Baune, BT Blair, S O'Sullivan, AJ Simar, D Singh, N Singh, MAF AF Simpson, Kylie A. Mavros, Yorgi Kay, Shelley Meiklejohn, Jacinda de Vos, Nathan Wang, Yi Guo, Qianyu Zhao, Renru Climstein, Mike Baune, Bernard T. Blair, Steven O'Sullivan, Anthony J. Simar, David Singh, Nalin Singh, Maria A. Fiatarone TI Graded Resistance Exercise And Type 2 Diabetes in Older adults (The GREAT2DO study): methods and baseline cohort characteristics of a randomized controlled trial SO TRIALS LA English DT Article DE Type 2 diabetes; Resistance training; Weight lifting; Randomized controlled trial; Power training ID BONE-MINERAL DENSITY; IMPROVES GLYCEMIC CONTROL; QUALITY-OF-LIFE; HIGH-INTENSITY; POSTMENOPAUSAL WOMEN; OVERWEIGHT ADULTS; PHYSICAL-ACTIVITY; FIBER-TYPE; POWER; STRENGTH AB Background: Type 2 diabetes (T2D) is projected to affect 439 million people by 2030. Medical management focuses on controlling blood glucose levels pharmacologically in a disease that is closely related to lifestyle factors such as diet and inactivity. Physical activity guidelines include aerobic exercise at intensities or volumes potentially unreachable for older adults limited by many co-morbidities. We aim to show for the first time the efficacy of a novel exercise modality, power training (high-velocity, high-intensity progressive resistance training or PRT), in older adults with T2D as a means for improving glycemic control and targeting many associated metabolic and physiological outcomes. Eligibility criteria included community-dwelling men and women previously diagnosed with T2D who met the current definition of metabolic syndrome according to the International Diabetes Federation. Participants were randomized to a fully supervised power training intervention or sham exercise control group for 12 months. Intervention group participants performed whole body machine-based power training at 80% 1RM, 3 days per week. The control group undertook the same volume of non-progressive, low-intensity training. Participants were assessed at baseline, 6 months and 12 months and followed for a further 5 years, during which time participants were advised to exercise at moderate-high intensity. Glycemic control (HbA1c) and insulin resistance as measured by the homeostatic model assessment 2 (HOMA2-IR) were the primary outcomes of the trial. Outcome assessors were blinded to group assignment and participants were blinded to the investigators' hypothesis regarding the most effective intervention. Results: We recruited 103 participants (48.5 % women, 71.6 +/- 5.6 years). Participants had 5.1 +/- 1.8 chronic diseases, had been diagnosed with T2D for 8 +/- 6 years and had a body mass index (BMI) of 31.6 +/- 4.0 kg/m(2). Fasting glucose and insulin were 7.3 +/- 2.4 mmol/L and 10.6 +/- 6.3 mU/L, respectively. HbA1c was 54 +/- 12 mmol/mol. Eighty-six participants completed the 12-month assessment and follow-up is ongoing. This cohort had a lower-than-expected dropout (n = 14, 14 %) over the 12-month intervention period. Conclusions: Power training may be a feasible adjunctive therapy for improving glycemic control for the growing epidemic of T2D in older adults. C1 [Simpson, Kylie A.; Mavros, Yorgi; Kay, Shelley; Meiklejohn, Jacinda; Guo, Qianyu; Zhao, Renru; Climstein, Mike; Singh, Nalin; Singh, Maria A. Fiatarone] Univ Sydney, Exercise Hlth & Performance Fac Res Grp, Fac Hlth Sci, Lidcombe, NSW 2750, Australia. [de Vos, Nathan] Balmain Hosp, Ctr STRONG Med, Balmain, NSW 2041, Australia. [Wang, Yi] Univ Calif San Francisco, Ctr Diabet, San Francisco, CA 94143 USA. [Baune, Bernard T.] Univ Adelaide, Royal Adelaide Hosp, Discipline Psychiat, Adelaide, SA 5005, Australia. [Blair, Steven] Univ S Carolina, Dept Exercise Sci, Columbia, SC 29208 USA. [O'Sullivan, Anthony J.] Univ New S Wales, St George Hosp, St George & Sutherland Clin Sch, Dept Med, Kogarah, NSW 2217, Australia. [Simar, David] Univ New S Wales, Metab Disorders Res Grp, Fac Med, Sydney, NSW 2052, Australia. [Singh, Maria A. Fiatarone] Univ Sydney, Sydney Med Sch, Sydney, NSW 2000, Australia. [Singh, Maria A. Fiatarone] Tufts Univ, Hebrew SeniorLife, Boston, MA 02111 USA. [Singh, Maria A. Fiatarone] Tufts Univ, Jean Mayer USDA Human Nutr Ctr Aging, Boston, MA 02111 USA. RP Simpson, KA (reprint author), Univ Sydney, Exercise Hlth & Performance Fac Res Grp, Fac Hlth Sci, 75 East St, Lidcombe, NSW 2750, Australia. EM kylie.simpson@sydney.edu.au OI Mavros, Yorgi/0000-0002-2588-0425; Simar, David/0000-0002-3862-1932 FU National Health and Medical Research Council (NHMRC) [512381]; Rebecca L. Cooper Foundation; Australian Postgraduate Award Scholarship; University of Sydney International Postgraduate Research Scholarship; Australian Diabetes Society; Diabetes Australia FX We would like to thank our participants for their generous contributions of time and spirit. We would like to thank Harbord Diggers' Freshwater Fitness Center and The STRONG Clinic at Balmain Hospital for the use of their gym facilities and Keiser Sports Health Ltd for donations of resistance training equipment. The Graded Resistance Exercise and Type 2 Diabetes in Older adults (GREAT2DO) study was funded by project grant #512381 from the National Health and Medical Research Council (NHMRC), grants from The Australian Diabetes Society, Diabetes Australia and the Rebecca L. Cooper Foundation. Y. Mavros was supported by the Australian Postgraduate Award Scholarship. Y. Wang was supported by the University of Sydney International Postgraduate Research Scholarship. NR 52 TC 1 Z9 1 U1 7 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1745-6215 J9 TRIALS JI Trials PD NOV 10 PY 2015 VL 16 AR 512 DI 10.1186/s13063-015-1037-y PG 14 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA CV7IH UT WOS:000364445100002 PM 26554457 ER PT J AU Wang, JD Gu, LQ Ireland, S Garczynski, SF Knipple, DC AF Wang, Jinda Gu, Liuqi Ireland, Stephen Garczynski, Stephen F. Knipple, Douglas C. TI Phenotypic screen for RNAi effects in the codling moth Cydia pomonella SO GENE LA English DT Article DE Cydia pomonella; Cullin; RNAi; siRNA ID DOUBLE-STRANDED-RNA; INTERFERENCE; MANAGEMENT; TRIBOLIUM; INSECTS; ELEGANS; FAMILY; GENES; DSRNA AB RNAi-based technologies have the potential to augment, or replace existing pest management strategies. However, some insect taxa are less susceptible to the induction of the post-transcriptional gene silencing effect than others, such as the Lepidoptera. Here we describe experiments to investigate the induction of RNAi in the codling moth, Cydia pomonella, a major lepidopteran pest of apple, pear, and walnut. Prior to a knockdown screen, fluorescently labeled small interfering RNA (siRNA) and double-stranded RNA (dsRNA) derived from green fluorescent protein (GFP) coding sequence were delivered to the surface of artificial diet to which neonate larvae were introduced and subsequently examined for the distribution of fluorescence in their tissues. Fluorescence was highly concentrated in the midgut but its presence in other tissues was equivocal. Next, dsRNAs were made for C. pomonella genes orthologous to those that have well defined deleterious phenotypes in Drosophila melanogaster. A screen was conducted using dsRNAs encoding cullin-1 (Cpcul1), maleless (Cpmle), musashi (Cpmsi), a homeobox gene (CpHbx), and pumilio (Cppum). The dsRNAs designed from these target genes were administered to neonate larvae by delivery to the surface of the growth medium. None of the dsRNA treatments affected larval viability, however Cpcul1-dsRNA had a significant effect on larval growth, with the average length of larvae about 3 mm, compared to about 4 mm in the control groups. Measurement of Cpcul1 transcript levels by quantitative real-time PCR (qRT-PCR) revealed a dose-dependent RNAi effect in response to increasing amount of Cpcul1-dsRNA. Despite their reduced size, Cpcul1-dsRNA-treated larvae molted normally and matured to adulthood in a manner similar to controls. In an additional experiment, Cpcul1-siRNA was found to induce similar stunting effect as that induced by Cpcul1-dsRNA. (C) 2015 Elsevier B.V. All rights reserved. C1 [Wang, Jinda; Gu, Liuqi; Ireland, Stephen; Knipple, Douglas C.] Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. [Wang, Jinda] Nanjing Agr Univ, Dept Entomol, Nanjing 210095, Jiangsu, Peoples R China. [Ireland, Stephen] Univ Michigan, Mol Cellular & Dev Biol, Ann Arbor, MI 48109 USA. [Garczynski, Stephen F.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Knipple, DC (reprint author), Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. EM dck2@cornell.edu FU China Scholarship Council [201306850026]; Cornell Department of Entomology [10334669]; Cornell Summer Scholars Internship grant [2011-12-158]; Washington Tree Fruit Research Commission [CP-13-01]; Hatch grants [6217499, 6217449] FX This work was supported by a China Scholarship Council grant (#201306850026) to JW, a Griswold Endowment Grant (#10334669) awarded through the Cornell Department of Entomology to LG, a Cornell Summer Scholars Internship grant (#2011-12-158) awarded to SI, a grant from the Washington Tree Fruit Research Commission (# CP-13-01) awarded to SFG, and Hatch grants (#6217499 and #6217449) to DCK. Thanks to Pofessor Zhaojun Han, advisor of JW at Nanjing Agricultural University, Nanjing, PRC. NR 23 TC 2 Z9 2 U1 0 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD NOV 10 PY 2015 VL 572 IS 2 BP 184 EP 190 DI 10.1016/j.gene.2015.07.006 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CS0SU UT WOS:000361773300005 PM 26162675 ER PT J AU Rinerson, CI Scully, ED Palmer, NA Donze-Reiner, T Rabara, RC Tripathi, P Shen, QXJ Sattler, SE Rohila, JS Sarath, G Rushton, PJ AF Rinerson, Charles I. Scully, Erin D. Palmer, Nathan A. Donze-Reiner, Teresa Rabara, Roel C. Tripathi, Prateek Shen, Qingxi J. Sattler, Scott E. Rohila, Jai S. Sarath, Gautam Rushton, Paul J. TI The WRKY transcription factor family and senescence in switchgrass SO BMC GENOMICS LA English DT Article ID ARABIDOPSIS LEAF SENESCENCE; VEGETATIVE TISSUES; RESPONSE ELEMENTS; PLANT SENESCENCE; NETWORK ANALYSIS; FLAG LEAVES; REVEALS; RICE; MAIZE; GENE AB Background: Early aerial senescence in switchgrass (Panicum virgatum) can significantly limit biomass yields. WRKY transcription factors that can regulate senescence could be used to reprogram senescence and enhance biomass yields. Methods: All potential WRKY genes present in the version 1.0 of the switchgrass genome were identified and curated using manual and bioinformatic methods. Expression profiles of WRKY genes in switchgrass flag leaf RNA-Seq datasets were analyzed using clustering and network analyses tools to identify both WRKY and WRKY-associated gene co-expression networks during leaf development and senescence onset. Results: We identified 240 switchgrass WRKY genes including members of the RW5 and RW6 families of resistance proteins. Weighted gene co-expression network analysis of the flag leaf transcriptomes across development readily separated clusters of co-expressed genes into thirteen modules. A visualization highlighted separation of modules associated with the early and senescence-onset phases of flag leaf growth. The senescence-associated module contained 3000 genes including 23 WRKYs. Putative promoter regions of senescence-associated WRKY genes contained several cis-element-like sequences suggestive of responsiveness to both senescence and stress signaling pathways. A phylogenetic comparison of senescence-associated WRKY genes from switchgrass flag leaf with senescence-associated WRKY genes from other plants revealed notable hotspots in Group I, IIb, and IIe of the phylogenetic tree. Conclusions: We have identified and named 240 WRKY genes in the switchgrass genome. Twenty three of these genes show elevated mRNA levels during the onset of flag leaf senescence. Eleven of the WRKY genes were found in hotspots of related senescence-associated genes from multiple species and thus represent promising targets for future switchgrass genetic improvement. Overall, individual WRKY gene expression profiles could be readily linked to developmental stages of flag leaves. C1 [Rinerson, Charles I.; Rabara, Roel C.; Rushton, Paul J.] Texas A&M AgriLife Res & Extens Ctr, Dallas, TX 75252 USA. [Scully, Erin D.; Palmer, Nathan A.; Sattler, Scott E.; Sarath, Gautam] Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Donze-Reiner, Teresa] West Chester Univ Penn, Dept Biol, W Chester, PA 19382 USA. [Tripathi, Prateek] Univ So Calif, Dana & David Dornsife Coll Letters Arts & Sci, Mol & Computat Biol Sect, Los Angeles, CA USA. [Shen, Qingxi J.] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA. [Rohila, Jai S.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. RP Sarath, G (reprint author), Univ Nebraska, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. EM Gautam.Sarath@ars.usda.gov; prushton@xxiicentury.com RI Tripathi, Prateek/L-2478-2016; TRIPATHI, PRATEEK/C-3883-2009; OI Tripathi, Prateek/0000-0002-0958-2428; TRIPATHI, PRATEEK/0000-0002-0958-2428; Rabara, Roel/0000-0001-8608-7060 FU Office of Science (BER), U.S. Department of Energy [DE-AI02-09ER64829]; USDA-NIFA [2011-67009-30096]; USDA-ARS CRIS [3042-21000-030-00D, 3042-21220-032-00D]; National Research Initiative from the USDA National Institute of Food and Agriculture [2008-35100-04519, 2008-35100-05969]; North Central Regional Sun Grant Center at South Dakota State University through US Department of Agriculture [2010-38502-21861] FX This work was supported in part by the Office of Science (BER), U.S. Department of Energy Grant Number DE-AI02-09ER64829, USDA-NIFA Grant Number 2011-67009-30096, and by the USDA-ARS CRIS projects 3042-21000-030-00D and 3042-21220-032-00D. The U.S. Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. The authors would like to thank Mani Kant Choudhary, Marissa Miller, Naveen Kumar, Malini Rao, Deena Rushton, Ying-Sheng Huang and Nikhil Kesarla in the Rushton lab. This project was supported in part by National Research Initiative grants 2008-35100-04519 and 2008-35100-05969 from the USDA National Institute of Food and Agriculture. This research was supported by funding from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Agriculture under award number 2010-38502-21861. NR 80 TC 5 Z9 6 U1 5 U2 26 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD NOV 9 PY 2015 VL 16 AR 912 DI 10.1186/s12864-015-2057-4 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CW8XD UT WOS:000365281800002 PM 26552372 ER PT J AU Chayut, N Yuan, H Ohali, S Meir, A Yeselson, Y Portnoy, V Zheng, Y Fei, ZJ Lewinsohn, E Katzir, N Schaffer, AA Gepstein, S Burger, J Li, L Tadmor, Y AF Chayut, Noam Yuan, Hui Ohali, Shachar Meir, Ayala Yeselson, Yelena Portnoy, Vitaly Zheng, Yi Fei, Zhangjun Lewinsohn, Efraim Katzir, Nurit Schaffer, Arthur A. Gepstein, Shimon Burger, Joseph Li, Li Tadmor, Yaakov TI A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit beta-carotene accumulation in melon fruit SO BMC PLANT BIOLOGY LA English DT Article DE Melon; Cucumis melo; Carotenoids; Beta-carotene; Bulk segregant analysis; CmOr; Fruit development; Transcriptome ID CUCUMIS-MELO; GENE-EXPRESSION; PHYTOENE SYNTHASE; TOMATO FRUIT; SUCROSE METABOLISM; TRANSGENIC POTATO; GENOMIC REGIONS; FLESH COLOR; CAULIFLOWER; BIOSYNTHESIS AB Background: Melon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of the "Orange" gene, CmOr, which dominantly triggers the accumulation of the pro-vitamin A molecule, beta-carotene, in the fruit mesocarp. The mechanism by which CmOr operates is not fully understood. To identify cellular and metabolic processes associated with CmOr allelic variation, we compared the transcriptome of bulks of developing fruit of homozygous orange and green fruited F-3 families derived from a cross between orange and green fruited parental lines. Results: Pooling together F-3 families that share same fruit flesh color and thus the same CmOr allelic variation, normalized traits unrelated to CmOr allelic variation. RNA sequencing analysis of these bulks enabled the identification of differentially expressed genes. These genes were clustered into functional groups. The relatively enriched functional groups were those involved in photosynthesis, RNA and protein regulation, and response to stress. Conclusions: The differentially expressed genes and the enriched processes identified here by bulk segregant RNA sequencing analysis are likely part of the regulatory network of CmOr. Our study demonstrates the resolution power of bulk segregant RNA sequencing in identifying genes related to commercially important traits and provides a useful tool for better understanding the mode of action of CmOr gene in the mediation of carotenoid accumulation. C1 [Chayut, Noam; Ohali, Shachar; Meir, Ayala; Portnoy, Vitaly; Lewinsohn, Efraim; Katzir, Nurit; Burger, Joseph; Tadmor, Yaakov] Agr Res Org, Plant Sci Inst, Newe Yaar Res Ctr, IL-30095 Ramat Yishay, Israel. [Chayut, Noam; Gepstein, Shimon] Technion Israel Inst Technol, Fac Biol, IL-32000 Haifa, Israel. [Yuan, Hui; Li, Li] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Yeselson, Yelena; Schaffer, Arthur A.] Agr Res Org, Volcani Ctr, Plant Sci Inst, IL-50250 Bet Dagan, Israel. [Li, Li] Cornell Univ, ARS, USDA, Robert W Holly Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Tadmor, Y (reprint author), Agr Res Org, Plant Sci Inst, Newe Yaar Res Ctr, POB 1021, IL-30095 Ramat Yishay, Israel. EM tadmory@agri.gov.il RI Yuan, Hui/H-6723-2016; Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU 'Center for the Improvement of Cucurbit Fruit Quality', ARO, Israel; [BARD US-4423-11] FX We gratefully acknowledge partial support from BARD US-4423-11 and from the 'Center for the Improvement of Cucurbit Fruit Quality', ARO, Israel. Publication No. 110/2015 of the Agricultural Research Organization, Bet Dagan, Israel. The authors greatly appreciate the technical assistance of Fabian Boumkoler, Uzi Sa'ar and Yunnis Ca'abeeya, for field and sampling assistance. NR 73 TC 3 Z9 3 U1 11 U2 40 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD NOV 9 PY 2015 VL 15 AR 274 DI 10.1186/s12870-015-0661-8 PG 18 WC Plant Sciences SC Plant Sciences GA CV5AP UT WOS:000364277900002 PM 26553015 ER PT J AU Katzner, TE Turk, PJ Duerr, AE Miller, TA Lanzone, MJ Cooper, JL Brandes, D Tremblay, JA Lemaitre, J AF Katzner, Todd E. Turk, Philip J. Duerr, Adam E. Miller, Tricia A. Lanzone, Michael J. Cooper, Jeff L. Brandes, David Tremblay, Junior A. Lemaitre, Jerome TI Use of multiple modes of flight subsidy by a soaring terrestrial bird, the golden eagle Aquila chrysaetos, when on migration SO JOURNAL OF THE ROYAL SOCIETY INTERFACE LA English DT Article DE Aquila chrysaetos; golden eagle; flight behaviour; migration; subsidized flight; weighted-k nearest neighbour ID BEHAVIOR; WIND; PERFORMANCE; THERMALS; RAPTORS; RADAR AB Large birds regularly use updrafts to subsidize flight. Although most research on soaring bird flight has focused on use of thermal updrafts, there is evidence suggesting that many species are likely to use multiple modes of subsidy. We tested the degree to which a large soaring species uses multiple modes of subsidy to provide insights into the decision-making that underlies flight behaviour. We statistically classified more than 22 000 global positioning satellite-global system for mobile communications telemetry points collected at 30-s intervals to identify the type of subsidized flight used by 32 migrating golden eagles during spring in eastern North America. Eagles used subsidized flight on 87% of their journey. They spent 41.9% +/- 1.5 ((x) over bar +/- s.e.m:, range: 18-56%) of their subsidized northbound migration using thermal soaring, 45.2%+/-2.1 (12-65%) of time gliding between thermals, and 12.9%+/-2.2 (1-55%) of time using orographic updrafts. Golden eagles responded to the variable local-scale meteorological events they encountered by switching flight behaviour to take advantage of multiple modes of subsidy. Orographic soaring occurred more frequently in morning and evening, earlier in the migration season, and when crosswinds and tail winds were greatest. Switching between flight modes allowed migration for relatively longer periods each day and frequent switching behaviour has implications for a better understanding of avian flight behaviour and of the evolution of use of subsidy in flight. C1 [Katzner, Todd E.; Duerr, Adam E.; Miller, Tricia A.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Katzner, Todd E.] US Forest Serv, USDA, No Res Stn, Parsons, WV 26287 USA. [Turk, Philip J.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Lanzone, Michael J.] Cellular Tracking Technol LLC, Somerset, PA 15501 USA. [Cooper, Jeff L.] Virginia Dept Game & Inland Fisheries, Fredericksburg, VA 22401 USA. [Brandes, David] Lafayette Coll, Dept Civil & Environm Engn, Easton, PA 18042 USA. [Tremblay, Junior A.] Environm Canada, Quebec City, PQ G1J 0C3, Canada. [Lemaitre, Jerome] Minist Forets Faune & Parcs, Quebec City, PQ G1S 4X4, Canada. RP Katzner, TE (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, 970 Lusk St, Boise, ID 83706 USA. EM tkatzner@usgs.gov OI Katzner, Todd/0000-0003-4503-8435 FU PA SWG [T-12, T47-R-1]; U.S. DoE [DE-EE0003538]; Charles A. and Anne Morrow Lindbergh Foundation; Penn State Earth and Environmental Institute Fellowship; Penn State Ecology Fellowship; Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Resources grant from the U.S. Fish and Wildlife Service FX Funding for this work was received from PA SWG grants T-12 and T47-R-1, U.S. DoE grant DE-EE0003538, Charles A. and Anne Morrow Lindbergh Foundation, Penn State Earth and Environmental Institute Fellowship, Penn State Ecology Fellowship, the Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Resources grant from the U.S. Fish and Wildlife Service, and the authors' organizations. NR 43 TC 4 Z9 4 U1 7 U2 20 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 1742-5689 EI 1742-5662 J9 J R SOC INTERFACE JI J. R. Soc. Interface PD NOV 6 PY 2015 VL 12 IS 112 AR 20150530 DI 10.1098/rsif.2015.0530 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV1BL UT WOS:000363987900002 ER PT J AU Schemerhorn, BJ Crane, YM Cambron, SE Crane, CF Shukle, RH AF Schemerhorn, Brandon J. Crane, Yan Ma Cambron, Sue E. Crane, Charles F. Shukle, Richard H. TI Use of Microsatellite and SNP Markers for Biotype Characterization in Hessian Fly SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Hessian fly; biotype; microsatellite; single-nucleotide polymorphism ID CECIDOMYIIDAE POPULATIONS; RESISTANCE GENES; UNITED-STATES; MAYETIOLA-DESTRUCTOR; WHEAT; DIPTERA; GENETICS; SOFTWARE; VIRULENCE; SOUTHEASTERN AB Exploration of the biotype structure of Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae), would improve our knowledge regarding variation in virulence phenotypes and difference in genetic background. Microsatellites (simple sequence repeats) and single-nucleotide polymorphisms (SNPs) are highly variable genetic markers that are widely used in population genetic studies. This study developed and tested a panel of 18 microsatellite and 22 SNP markers to investigate the genetic structure of nine Hessian fly biotypes: B, C, D, E, GP, L, O, vH9, and vH13. The simple sequence repeats were more polymorphic than the SNP markers, and their neighbor-joining trees differed in consequence. Microsatellites suggested a simple geographic association of related biotypes that did not progressively gain virulence with increasing genetic distance from a founder type. Use of the k-means clustering algorithm in the STRUCTURE program shows that the nine biotypes comprise six to eight populations that are related to geography or history within laboratory cultures. C1 [Schemerhorn, Brandon J.; Crane, Yan Ma; Cambron, Sue E.; Crane, Charles F.; Shukle, Richard H.] ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Schemerhorn, Brandon J.; Crane, Yan Ma; Cambron, Sue E.; Shukle, Richard H.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Crane, Charles F.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. RP Schemerhorn, BJ (reprint author), ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. EM bschemer@purdue.edu FU USDA-ARS [3602-22000-018-00D] FX This work was supported by USDA-ARS CRIS no. 3602-22000-018-00D. We would like to acknowledge the assistance of Richard Smith. Mention of a commercial or proprietary product does not constitute an endorsement by the USDA. NR 40 TC 0 Z9 0 U1 2 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD NOV 5 PY 2015 VL 15 AR 158 DI 10.1093/jisesa/iev138 PG 8 WC Entomology SC Entomology GA CV5KU UT WOS:000364310000001 ER PT J AU Yadav, MP Hicks, KB AF Yadav, Madhav P. Hicks, Kevin B. TI Isolation of barley hulls and straw constituents and study of emulsifying properties of their arabinoxylans SO CARBOHYDRATE POLYMERS LA English DT Article DE Barley hulls; Barley straws; Emulsifying activity; Emulsion stability; Emulsifier; Oil-in-water emulsion ID CORN FIBER GUM; CELL-WALL COMPONENTS; EMULSIFICATION; EXTRACTION; HUSKS; WHEAT; HEMICELLULOSES; MATURATION; CULM AB Both barley hulls and straw contain valuable arabinoxylans and other useful carbohydrate and non-carbohydrate components. The functional water soluble non-caloric arabinoxylan (hemicellulose B) fraction was isolated from hot water-extracted and de-starched barley hulls and straws by an alkaline hydrogen peroxide extraction followed by ethanol precipitation. Barley hulls contained comparatively more Hemi. B (20.51%) than barley straws (7.41 to 12.94%). The sugar composition of Hemi. B showed that they were typical arabinoxylans containing (in addition to arabinose and xylose) some galactose, glucose and acidic sugars in the side chains. The hemicellulose B fractions from barley straws were superior oil-in-water emulsifiers than those from barley hulls. These Hemi. B fractions contain protein, which contributes to their emulsions stabilizing property. Published by Elsevier Ltd. C1 [Yadav, Madhav P.; Hicks, Kevin B.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Yadav, MP (reprint author), ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM madhav.yadav@ars.usda.gov NR 38 TC 2 Z9 2 U1 7 U2 27 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD NOV 5 PY 2015 VL 132 BP 529 EP 536 DI 10.1016/j.carbpol.2015.06.049 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA CR2UA UT WOS:000361184900063 PM 26256379 ER PT J AU Maxwell, EG Colquhoun, IJ Chau, HK Hotchkiss, AT Waldron, KW Morris, VJ Belshaw, NJ AF Maxwell, Ellen G. Colquhoun, Ian J. Chau, Hoa K. Hotchkiss, Arland T. Waldron, Keith W. Morris, Victor J. Belshaw, Nigel J. TI Rhamnogalacturonan I containing homogalacturonan inhibits colon cancer cell proliferation by decreasing ICAM1 expression SO CARBOHYDRATE POLYMERS LA English DT Article DE Modified pectin; Rhamnogalacturonan I; Homogalacturonan; Galactan; Colon cancer; ICAM1 ID MODIFIED CITRUS PECTIN; GINSENG PECTIN; GALECTIN-3; POLYSACCHARIDES; INDUCTION; APOPTOSIS; ADHESION; REGIONS AB Pectin modified with pH, heat or enzymes, has previously been shown to exhibit anti-cancer activity. However, the structural requirements for modified pectin bioactivity have rarely been addressed. In this study several pectin extracts representing different structural components of pectin were assessed for effects against colon cancer cells. Rhamnogalacturonan I (RGI) extracts reduced proliferation of DLD1 and HCT116 colon cancer cells in a dose- and time-dependent manner. RGI reduced ICAM1 gene expression and siRNA-mediated knockdown of ICAM1 expression decreased cell proliferation providing a potential novel mechanism for the anti-cancer activity of pectin. Structural analysis of bioactive and non-bioactive RGIs suggested that a homogalacturonan component is maybe essential for the anti-proliferative activity, furthering the understanding of the structural requirements for pectin bioactivity. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Maxwell, Ellen G.; Colquhoun, Ian J.; Waldron, Keith W.; Morris, Victor J.; Belshaw, Nigel J.] Inst Food Res, Norwich NR4 7UA, Norfolk, England. [Chau, Hoa K.; Hotchkiss, Arland T.] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA. RP Belshaw, NJ (reprint author), Inst Food Res, Norwich Res Pk, Norwich NR4 7UA, Norfolk, England. EM ellen.maxwell@ifr.ac.uk; ian.colquhoun@ifr.ac.uk; hoa.chau@ars.usda.gov; arland.hotchkiss@ars.usda.gov; keith.waldron@ifr.ac.uk; vic.morris@ifr.ac.uk; nigel.belshaw@ifr.ac.uk FU International Pectin Producers Association; UK Biotechnology and Biological Sciences Research Council's core strategic grant [BB/J004529/1, BB/J004545/1] FX This work was supported by the International Pectin Producers Association and by the UK Biotechnology and Biological Sciences Research Council's core strategic grant to the Institute of Food Research's Gut Health and Food Safety (BB/J004529/1) and Food and Health (BB/J004545/1) programmes. EGM wishes to thank Dr R Bongaerts for an introduction to flow cytometry techniques. NR 29 TC 6 Z9 6 U1 4 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD NOV 5 PY 2015 VL 132 BP 546 EP 553 DI 10.1016/j.carbpol.2015.06.082 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA CR2UA UT WOS:000361184900065 PM 26256381 ER PT J AU Docherty, KM Borton, HM Espinosa, N Gebhardt, M Gil-Loaiza, J Gutknecht, JLM Maes, PW Mott, BM Parnell, JJ Purdy, G Rodrigues, PAP Stanish, LF Walser, ON Gallery, RE AF Docherty, Kathryn M. Borton, Hannah M. Espinosa, Noelle Gebhardt, Martha Gil-Loaiza, Juliana Gutknecht, Jessica L. M. Maes, Patrick W. Mott, Brendon M. Parnell, John Jacob Purdy, Gayle Rodrigues, Pedro A. P. Stanish, Lee F. Walser, Olivia N. Gallery, Rachel E. TI Key Edaphic Properties Largely Explain Temporal and Geographic Variation in Soil Microbial Communities across Four Biomes SO PLOS ONE LA English DT Article ID BACTERIAL COMMUNITY; DIVERSITY; FORESTS; BIOMASS; BIOGEOGRAPHY; PHYSIOLOGY; SEQUENCES; GRASSLAND; DORMANCY; DYNAMICS AB Soil microbial communities play a critical role in nutrient transformation and storage in all ecosystems. Quantifying the seasonal and long-term temporal extent of genetic and functional variation of soil microorganisms in response to biotic and abiotic changes within and across ecosystems will inform our understanding of the effect of climate change on these processes. We examined spatial and seasonal variation in microbial communities based on 16S rRNA gene sequencing and phospholipid fatty acid (PLFA) composition across four biomes: a tropical broadleaf forest (Hawaii), taiga (Alaska), semiarid grassland-shrubland (Utah), and a subtropical coniferous forest (Florida). In this study, we used a team-based instructional approach leveraging the iPlant Collaborative to examine publicly available National Ecological Observatory Network (NEON) 16S gene and PLFA measurements that quantify microbial diversity, composition, and growth. Both profiling techniques revealed that microbial communities grouped strongly by ecosystem and were predominately influenced by three edaphic factors: pH, soil water content, and cation exchange capacity. Temporal variability of microbial communities differed by profiling technique; 16S-based community measurements showed significant temporal variability only in the subtropical coniferous forest communities, specifically through changes within subgroups of Acidobacteria. Conversely, PLFA-based community measurements showed seasonal shifts in taiga and tropical broadleaf forest systems. These differences may be due to the premise that 16S-based measurements are predominantly influenced by large shifts in the abiotic soil environment, while PLFA-based analyses reflect the metabolically active fraction of the microbial community, which is more sensitive to local disturbances and biotic interactions. C1 [Docherty, Kathryn M.; Borton, Hannah M.; Walser, Olivia N.] Western Michigan Univ, Dept Biol Sci, Kalamazoo, MI 49008 USA. [Espinosa, Noelle; Gebhardt, Martha; Purdy, Gayle; Gallery, Rachel E.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Gil-Loaiza, Juliana] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ USA. [Gutknecht, Jessica L. M.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Maes, Patrick W.; Rodrigues, Pedro A. P.] Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, Tucson, AZ USA. [Mott, Brendon M.] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ USA. [Parnell, John Jacob; Stanish, Lee F.] Natl Ecol Observ Network, Boulder, CO USA. [Gallery, Rachel E.] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ USA. RP Gallery, RE (reprint author), Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. EM rgallery@email.arizona.edu OI Rodrigues, Pedro/0000-0002-3280-7576 FU National Science Foundation-EAR [1331408]; National Institute of Food and Agriculture [ARZT-1360540H12-199] FX The authors received no specific funding for this work. REG acknowledges the National Science Foundation-EAR Award Number 1331408: Transformative Behavior of Energy, Water and Carbon in the Critical Zone II and National Institute of Food and Agriculture Award Number ARZT-1360540H12-199. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 3 Z9 4 U1 7 U2 46 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 4 PY 2015 VL 10 IS 11 AR e0135352 DI 10.1371/journal.pone.0135352 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV5GZ UT WOS:000364298400003 PM 26536666 ER PT J AU Gabriel, MW Woods, LW Wengert, GM Stephenson, N Higley, JM Thompson, C Matthews, SM Sweitzer, RA Purcell, K Barrett, RH Keller, SM Gaffney, P Jones, M Poppenga, R Foley, JE Brown, RN Clifford, DL Sacks, BN AF Gabriel, Mourad W. Woods, Leslie W. Wengert, Greta M. Stephenson, Nicole Higley, J. Mark Thompson, Craig Matthews, Sean M. Sweitzer, Rick A. Purcell, Kathryn Barrett, Reginald H. Keller, Stefan M. Gaffney, Patricia Jones, Megan Poppenga, Robert Foley, Janet E. Brown, Richard N. Clifford, Deana L. Sacks, Benjamin N. TI Patterns of Natural and Human-Caused Mortality Factors of a Rare Forest Carnivore, the Fisher (Pekania pennanti) in California SO PLOS ONE LA English DT Article ID MOUNTAIN LION PREDATION; MARTES-PENNANTI; TOXOPLASMA-GONDII; CANINE-DISTEMPER; DNA; POPULATION; MAMMALS; RISK AB Wildlife populations of conservation concern are limited in distribution, population size and persistence by various factors, including mortality. The fisher (Pekania pennanti), a North American mid-sized carnivore whose range in the western Pacific United States has retracted considerably in the past century, was proposed for threatened status protection in late 2014 under the United States Endangered Species Act by the United States Fish and Wildlife Service in its West Coast Distinct Population Segment. We investigated mortality in 167 fishers from two genetically and geographically distinct sub-populations in California within this West Coast Distinct Population Segment using a combination of gross necropsy, histology, toxicology and molecular methods. Overall, predation (70%), natural disease (16%), toxicant poisoning (10%) and, less commonly, vehicular strike (2%) and other anthropogenic causes (2%) were causes of mortality observed. We documented both an increase in mortality to (57% increase) and exposure (6%) from pesticides in fishers in just the past three years, highlighting further that toxicants from marijuana cultivation still pose a threat. Additionally, exposure to multiple rodenticides significantly increased the likelihood of mortality from rodenticide poisoning. Poisoning was significantly more common in male than female fishers and was 7 times more likely than disease to kill males. Based on necropsy findings, suspected causes of mortality based on field evidence alone tended to underestimate the frequency of disease-related mortalities. This study is the first comprehensive investigation of mortality causes of fishers and provides essential information to assist in the conservation of this species. C1 [Gabriel, Mourad W.; Wengert, Greta M.] Integral Ecol Res Ctr, Blue Lake, CA 95525 USA. [Gabriel, Mourad W.; Sacks, Benjamin N.] Univ Calif Davis, Mammalian Ecol & Conservat Unit, Vet Genet Lab, Davis, CA 95616 USA. [Woods, Leslie W.; Poppenga, Robert] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Davis, CA 95616 USA. [Stephenson, Nicole; Foley, Janet E.] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA. [Higley, J. Mark] Hoopa Tribal Forestry, Dept Wildlife, Hoopa, CA USA. [Thompson, Craig; Purcell, Kathryn] US Forest Serv, Pacific Southwest Res Stn, Sierra Nevada Res Ctr, Fresno, CA USA. [Matthews, Sean M.] Wildlife Conservat Soc, Hoopa, CA USA. [Sweitzer, Rick A.; Barrett, Reginald H.] Univ Calif Berkeley, Sierra Nevada Adapt Management Project, Berkeley, CA 94720 USA. [Keller, Stefan M.; Gaffney, Patricia; Jones, Megan] Univ Calif Davis, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. [Brown, Richard N.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Clifford, Deana L.] Calif Dept Fish & Wildlife, Wildlife Invest Lab, Rancho Cordova, CA USA. [Sacks, Benjamin N.] Univ Calif Davis, Dept Populat Hlth & Reprod, Davis, CA 95616 USA. RP Gabriel, MW (reprint author), Integral Ecol Res Ctr, Blue Lake, CA 95525 USA. EM mgabriel@IERCecology.org RI Purcell, Kathryn/S-2592-2016 FU Integral Ecology Research Center; California Animal Health and Food Safety Laboratory System; Hoopa Valley Tribal Forestry; United States Forest Service; National Park Service; United States Fish and Wildlife Service; California Department of Water Resources; California Department of Fish and Wildlife; California Department of Forestry and Fire Protection; Sierra Nevada Conservancy; Sierra Nevada Adaptive Management Project; Bureau of Indian Affairs FX Funding: This work was supported by the Integral Ecology Research Center, California Animal Health and Food Safety Laboratory System, Hoopa Valley Tribal Forestry, United States Forest Service, National Park Service, United States Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Wildlife, California Department of Forestry and Fire Protection, The Sierra Nevada Conservancy, Sierra Nevada Adaptive Management Project and the Bureau of Indian Affairs. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 1 Z9 1 U1 20 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 4 PY 2015 VL 10 IS 11 AR e0140640 DI 10.1371/journal.pone.0140640 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV5GZ UT WOS:000364298400028 PM 26536481 ER PT J AU Wilcox, TM Carim, KJ McKelvey, KS Young, MK Schwartz, MK AF Wilcox, Taylor M. Carim, Kellie J. McKelvey, Kevin S. Young, Michael K. Schwartz, Michael K. TI The Dual Challenges of Generality and Specificity When Developing Environmental DNA Markers for Species and Subspecies of Oncorhynchus SO PLOS ONE LA English DT Article ID YELLOWSTONE CUTTHROAT TROUT; WESTERN UNITED-STATES; CONSERVATION AB Environmental DNA (eDNA) sampling is a powerful tool for detecting invasive and native aquatic species. Often, species of conservation interest co-occur with other, closely related taxa. Here, we developed qPCR (quantitative PCR) markers which distinguish westslope cutthroat trout (Oncorhynchus clarkii lewsi), Yellowstone cutthroat trout (O. clarkii bouvieri), and rainbow trout (O. mykiss), which are of conservation interest both as native species and as invasive species across each other's native ranges. We found that local polymorphisms within westslope cutthroat trout and rainbow trout posed a challenge to designing assays that are generally applicable across the range of these widely-distributed species. Further, poorly-resolved taxonomies of Yellowstone cutthroat trout and Bonneville cutthroat trout (O. c. utah) prevented design of an assay that distinguishes these recognized taxa. The issues of intraspecific polymorphism and unresolved taxonomy for eDNA assay design addressed in this study are likely to be general problems for closely-related taxa. Prior to field application, we recommend that future studies sample populations and test assays more broadly than has been typical of published eDNA assays to date. C1 [Wilcox, Taylor M.; Carim, Kellie J.; McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K.] US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT 59812 USA. [Wilcox, Taylor M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Wilcox, TM (reprint author), US Forest Serv, USDA, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, Missoula, MT 59812 USA. EM taylor.wilcox@umontana.edu FU National Science Foundation [DGE-1313190] FX Region 1 of the U.S. Forest Service provided funding for this work. TMW is supported by a fellowship from the National Science Foundation (Grant No. DGE-1313190). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 29 TC 7 Z9 7 U1 7 U2 45 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 4 PY 2015 VL 10 IS 11 AR e0142008 DI 10.1371/journal.pone.0142008 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV5GZ UT WOS:000364298400117 PM 26536367 ER PT J AU Rimando, AM Mahattanatawee, K AF Rimando, Agnes M. Mahattanatawee, Kanjana TI The First Joint ACS AGFD and ACS ICSCT Symposium SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Editorial Material C1 [Rimando, Agnes M.] USDA, Agr Res Serv, Thad Cochran Res Ctr, University, MS 38677 USA. [Mahattanatawee, Kanjana] Siam Univ, Fac Sci, Bangkok 10160, Thailand. RP Rimando, AM (reprint author), USDA, Agr Res Serv, Thad Cochran Res Ctr, 106 Univ Ave, University, MS 38677 USA. EM agnes.rimando@ars.usda.gov NR 7 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 4 PY 2015 VL 63 IS 43 BP 9379 EP 9380 DI 10.1021/jf504201z PG 2 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CV6BM UT WOS:000364355700001 PM 26458975 ER PT J AU Tunick, MH Van Hekken, DL AF Tunick, Michael H. Van Hekken, Diane L. TI Dairy Products and Health: Recent Insights SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 1st ACS-AGFD and ACSThailand Chapter Joint Symposium CY MAR 04-05, 2014 CL Bangkok, THAILAND DE dairy; health; nutrition ID CONJUGATED LINOLEIC-ACID; RANDOMIZED CONTROLLED-TRIALS; COLON-CANCER CELLS; WHEY-PROTEIN; RESISTANCE EXERCISE; BODY-COMPOSITION; BLOOD-PRESSURE; MILK-PRODUCTS; VACCENIC ACID; FATTY-ACIDS AB Milk, cheese, yogurt, and other dairy products have long been known to provide good nutrition. Major healthful contributors to the diets of many people include the protein, minerals, vitamins, and fatty acids present in milk. Recent studies have shown that consumption of dairy products appears to be beneficial in muscle building, lowering blood pressure and low-density lipoprotein cholesterol, and preventing tooth decay, diabetes, cancer, and obesity. Additional benefits might be provided by organic milk and by probiotic microorganisms using milk products as a vehicle. New research on dairy products and nutrition will improve our understanding of the connections between these products, the bioactive compounds in them, and their effects on the human body. C1 [Tunick, Michael H.; Van Hekken, Diane L.] USDA, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, Agr Res Serv, Wyndmoor, PA 19038 USA. RP Tunick, MH (reprint author), USDA, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, Agr Res Serv, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM michael.tunick@ars.usda.gov NR 84 TC 6 Z9 6 U1 7 U2 85 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 4 PY 2015 VL 63 IS 43 BP 9381 EP 9388 DI 10.1021/jf5042454 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CV6BM UT WOS:000364355700002 PM 25394286 ER PT J AU Green, PWC Davis, AP Cosse, AA Vega, FE AF Green, Paul W. C. Davis, Aaron P. Cosse, Allard A. Vega, Fernando E. TI Can Coffee Chemical Compounds and Insecticidal Plants Be Harnessed for Control of Major Coffee Pests? SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 1st ACS-AGFD and ACSThailand Chapter Joint Symposium CY MAR 04-05, 2014 CL Bangkok, THAILAND DE Hypothenemus hampei; Coffea arabica; Coffea canephora; coffee chemistry; coffee pests; pest control; pesticidal plants ID HYPOTHENEMUS-HAMPEI COLEOPTERA; TEREBINTHIFOLIUS-RADDI ANACARDIACEAE; NERIUM-OLEANDER LEAVES; BERRY BORER; ESSENTIAL OIL; BEAUVERIA-BASSIANA; GREEN COFFEE; CHLOROGENIC ACIDS; BEVERAGE QUALITY; ARABICA COFFEE AB Pests and pathogens threaten coffee production worldwide and are difficult to control using conventional methods, such as insecticides. We review the literature on the chemistry of coffee, concentrating on compounds most commonly reported from Coffea arabica and Coffea canephora. Differences in chemistry can distinguish coffee species and varieties, and plants grown under different biogeographic conditions exhibit different chemotypes. A number of chemical groups, such as alkaloids and caffeoylquinic acids, are known to be insecticidal, but most studies have investigated their effects on coffee quality and flavor. More research is required to bridge this gap in knowledge, so that coffee can be bred to be more resistant to pests. Furthermore, we report on some pesticidal plants that have been used for control of coffee pests. Locally sourced pesticidal plants have been underutilized and offer a sustainable alternative to conventional insecticides and could be used to augment breeding for resilience of coffee plants. C1 [Green, Paul W. C.; Davis, Aaron P.] Royal Bot Gardens, Richmond TW9 3AB, Surrey, England. [Cosse, Allard A.] ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Vega, Fernando E.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. RP Green, PWC (reprint author), Royal Bot Gardens, Richmond TW9 3AB, Surrey, England. EM paul.green@kew.org RI Green, Paul/G-5116-2012; OI Green, Paul/0000-0001-7140-7121; Vega, Fernando E./0000-0001-8103-5640 NR 144 TC 1 Z9 1 U1 6 U2 33 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 4 PY 2015 VL 63 IS 43 BP 9427 EP 9434 DI 10.1021/acs.jafc.5b03914 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CV6BM UT WOS:000364355700007 PM 26458882 ER PT J AU Glahn, RP Cheng, ZQ Giri, S AF Glahn, Raymond P. Cheng, Zhiqiang Giri, Shree TI Extrinsic Labeling of Staple Food Crops with Isotopic Iron Does Not Consistently Result in Full Equilibration: Revisiting the Methodology SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 1st ACS-AGFD and ACSThailand Chapter Joint Symposium CY MAR 04-05, 2014 CL Bangkok, THAILAND DE extrinsic iron labeling; iron bioavailability; isotopic exchange ID PHASEOLUS-VULGARIS L.; YOUNG-WOMEN; IN-VITRO; ABSORBABLE IRON; RWANDESE WOMEN; PEARL-MILLET; CACO-2 CELL; BIOAVAILABILITY; ABSORPTION; BEANS AB Extrinsic isotopic labeling of food Fe has been used for over 50 years to measure Fe absorption. This method assumes that complete equilibration occurs between the extrinsic and the intrinsic Fe prior to intestinal absorption. The present study tested this assumption via in vitro digestion of varieties of maize, white beans, black beans, red beans, and lentils. Prior to digestion, foods were extrinsically labeled with Fe-58 at concentrations of 1, 10, 50, and 100% of the intrinsic Fe-56. Following an established in vitro digestion protocol, the digest was centrifuged and the Fe solubilities of the extrinsic Fe-58 and the intrinsic Fe-56 were compared as a measure of extrinsic/intrinsic equilibration. In the beans, significantly more of the extrinsic Fe (up to 2-3 times, p < 0.001) partitioned into the supernatant. The effect varied depending upon the seed coat color, the harvest, and the concentration of the extrinsic Fe. For lentils and maize the extrinsic Fe tended to partition into the insoluble fraction and also varied depending on variety and harvest. There was no crop that consistently demonstrated full equilibration of the extrinsic Fe with the intrinsic Fe. These observations challenge the accuracy of Fe absorption studies in which isotopic extrinsic Fe was used to evaluate Fe absorption and bioavailability. C1 [Glahn, Raymond P.] ARS, Robert Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. [Cheng, Zhiqiang; Giri, Shree] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Glahn, RP (reprint author), ARS, Robert Holley Ctr Agr & Hlth, USDA, 538 Tower Rd, Ithaca, NY 14853 USA. EM Raymond.glahn@ars.usda.gov FU USDA-AIRS FX This research was funded by USDA-AIRS. NR 38 TC 2 Z9 2 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 4 PY 2015 VL 63 IS 43 BP 9621 EP 9628 DI 10.1021/acs.jafc.5b03926 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CV6BM UT WOS:000364355700031 PM 26456842 ER PT J AU Smith, DJ Ernst, W Herges, GR AF Smith, D. J. Ernst, W. Herges, G. R. TI Chloroxyanion Residues in Cantaloupe and Tomatoes after Chlorine Dioxide Gas Sanitation SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 1st ACS-AGFD and ACSThailand Chapter Joint Symposium CY MAR 04-05, 2014 CL Bangkok, THAILAND DE chlorine dioxide; chlorate; perchlorate; chlorite; residue; food safety ID TOTAL RADIOACTIVE RESIDUES; ESCHERICHIA-COLI O157-H7; TANDEM MASS-SPECTROMETRY; SODIUM CL-36 CHLORATE; LISTERIA-MONOCYTOGENES; KILLING SALMONELLA; PERCHLORATE ANION; AQUEOUS-SOLUTION; PARENT CHLORATE; DRINKING-WATER AB Chlorine dioxide gas is effective at cleansing fruits and vegetables of bacterial pathogens and(or) rot organisms, but little data are available on chemical residues remaining subsequent to chlorine gas treatment. Therefore, studies were conducted to quantify chlorate and perchlorate residues after tomato and cantaloupe treatment with chlorine dioxide gas. Treatments delivered 50 mg of chlorine dioxide gas per kg of tomato (2-h treatment) and 100 mg of gas per kg of cantaloupe (6-h treatment) in sealed, darkened containers. Chlorate residues in tomato and cantaloupe edible flesh homogenates were less than the LC-MS/MS limit of quantitation (60 and 30 ng/g respectively), but were 1319 +/- 247 ng/g in rind + edible flesh of cantaloupe. Perchlorate residues in all fractions of chlorine dioxide-treated tomatoes and cantaloupe were not different (P > 0.05) than perchlorate residues in similar fractions of untreated tomatoes and cantaloupe. Data from this study suggest that chlorine dioxide sanitation of edible vegetables and melons can be conducted without the formation of unwanted residues in edible fractions. C1 [Smith, D. J.; Herges, G. R.] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. [Ernst, W.] ICA Trinova Corp LLC, Newnan, GA 30263 USA. RP Smith, DJ (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM david.j.smith@ars.usda.gov NR 71 TC 1 Z9 1 U1 5 U2 14 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD NOV 4 PY 2015 VL 63 IS 43 BP 9640 EP 9649 DI 10.1021/acs.jafc.5b04153 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CV6BM UT WOS:000364355700033 PM 26496046 ER PT J AU Samayoa, LF Malvar, RA McMullen, MD Butron, A AF Fernando Samayoa, Luis Ana Malvar, Rosa McMullen, Michael D. Butron, Ana TI Identification of QTL for resistance to Mediterranean corn borer in a maize tropical line to improve temperate germplasm SO BMC PLANT BIOLOGY LA English DT Article DE Maize; Corn borer; Quantitative trait loci; Insect resistance; Cross validation; Sesamia nonagrioides; Marker assisted selection ID QUANTITATIVE TRAIT LOCI; OSTRINIA-NUBILALIS HB.; MAYS L. GERMPLASM; PINK STEM BORER; SESAMIA-NONAGRIOIDES; INDEPENDENT SAMPLES; GENETIC-VARIATION; CROSS-VALIDATION; GENOMIC REGIONS; GRAIN-YIELD AB Background: A QTL mapping study for maize resistance to the Mediterranean corn borer (MCB) was performed with a RIL population derived from the cross B73 x CML103. To develop commercial inbreds of maize resistant to the MCB for use in Europe, it would be useful to transfer resistance from tropical germplasm like the subtropical inbred CML103 to temperate lines. The inbred B73 was chosen as representative of the Stiff Stock heterotic group, a major heterotic group used in hybrid grown in both North American and Europe. The objectives were to study the architecture of genetic factors for resistance to MCB and to check the feasibility of using marker-assisted selection (MAS) for transferring those genetic factors. Results: Eight quantitative trait loci (QTL) were declared significant for resistance traits and eight QTL were located for agronomic traits. Alleles from CML103 at QTL significant for tunnel length could reduce tunnel length made for MCB in inbred B73 in more than 8 cm; favorable alleles for yield were also found in CML103 and no genetic correlation coefficient between tunnel length and yield was detected. Conclusions: MAS for transferring resistance genes to corn borer attack from CML103 to B73 could be successful based on cross validation results and a negative effect on yield would not be expected. C1 [Fernando Samayoa, Luis; Ana Malvar, Rosa; Butron, Ana] CSIC, Mision Biol Galicia, Pontevedra 36080, Spain. [McMullen, Michael D.] Univ Missouri, Plant Sci Res Unit, USDA Agr Res Serv, Columbia, MO 65211 USA. [McMullen, Michael D.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Samayoa, LF (reprint author), CSIC, Mision Biol Galicia, POB 28, Pontevedra 36080, Spain. EM fsamayoa@mbg.csic.es FU National Plan for Research and Development of Spain - European Regional Development Fund [AGL2009-09611, AGL2012-33415]; Spanish Council for Scientific Research (CSIC); USDA-ARS FX This work was supported by the National Plan for Research and Development of Spain (projects AGL2009-09611 and AGL2012-33415, both funded by the European Regional Development Fund). L.F. Samayoa acknowledges a contract JAE-Predoc from the Spanish Council for Scientific Research (CSIC). MDM acknowledges research support by USDA-ARS. NR 51 TC 0 Z9 0 U1 15 U2 47 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD NOV 4 PY 2015 VL 15 AR 265 DI 10.1186/s12870-015-0652-9 PG 9 WC Plant Sciences SC Plant Sciences GA CV5AJ UT WOS:000364277300001 PM 26530038 ER PT J AU Harris, C Abubeker, S Yu, MM Leskey, T Zhang, AJ AF Harris, Christina Abubeker, Sitra Yu, Mengmeng Leskey, Tracy Zhang, Aijun TI Semiochemical Production and Laboratory Behavior Response of the Brown Marmorated Stink Bug, Halyomorpha Halys SO PLOS ONE LA English DT Article ID SUBSTRATE-BORNE SIGNALS; HETEROPTERA-PENTATOMIDAE; SEX-PHEROMONE; AGGREGATION PHEROMONE; MOTH; IDENTIFICATION; COMMUNICATION; ATTRACTION; HEMIPTERA AB Background The brown marmorated stink bug (BMSB) is an exotic insect pest that was first recognized in the United States in 2001. As of today, it has been found in more than 42 states. BMSB has a very broad host plant range and damage to crops in mid-Atlantic States has reached a critical level. A reliable and accurate tool for infestation detection and population monitoring is urgently needed to provide better and more timely interventions. Pheromones produced by male BMSB have been previously identified and are currently used in BMSB infestation detection. However, the conditions affecting BMSB production of these pheromones were unknown. Methodology/Principal Findings In this study, we collected headspace volatiles from male BMSB under laboratory conditions, measured the temporal patterns of release of these pheromones, and assayed the attractiveness to conspecifics. In addition to the pheromone components, tridecane (C13) and E-2-decenal (an alarm compound) were observed in headspace collections of males, as well as in females and nymphs. Exposure of pheromone-emitting adult males to synthetic C13 greatly reduced pheromone emission. Conclusions/Significance This information should lead to a better understanding of the biology, physiology, and chemical ecology of BMSB, which will help scientists and growers develop more efficient strategies based on natural products to manage BMSB population, therefore, reducing pesticide usage and protecting the crops from BMSB damage. C1 [Harris, Christina; Abubeker, Sitra; Yu, Mengmeng; Zhang, Aijun] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. [Harris, Christina] Virginia Tech Univ, Dept Entomol, Blacksburg, VA 24060 USA. [Leskey, Tracy] ARS, Appalachian Fruit Res Stn, USDA, Kearneysville, WV 25430 USA. RP Zhang, AJ (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. EM aijun.zhang@ars.usda.gov FU USDA FX This research was fully funded by USDA. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 2 Z9 2 U1 5 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD NOV 3 PY 2015 VL 10 IS 11 AR e0140876 DI 10.1371/journal.pone.0140876 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV1QH UT WOS:000364032600016 PM 26528717 ER PT J AU Homann, PS Darbyshire, RL Bormann, BT Morrissette, BA AF Homann, Peter S. Darbyshire, Robyn L. Bormann, Bernard T. Morrissette, Brett A. TI Forest Structure Affects Soil Mercury Losses in the Presence and Absence of Wildfire SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID UNITED-STATES; CONIFEROUS FORESTS; PACIFIC-NORTHWEST; PRESCRIBED FIRE; WET DEPOSITION; SHORT-TERM; NITROGEN; CARBON; CLIMATE; USA AB Soil is an important, dynamic component of regional and global mercury (Hg) cycles. This study evaluated how changes in forest soil Hg masses caused by atmospheric deposition and wildfire are affected by forest structure. Pre and postfire soil Hg measurements were made over two decades on replicate experimental units of three prefire forest structures (mature unthinned, mature thinned, clear-cut) in Douglas-fir dominated forest of southwestern Oregon. In the absence of wildfire, O-horizon Hg decreased by 60% during the 14 years after clearcutting, possibly the result of decreased atmospheric deposition due to the smaller-stature vegetative canopy; in contrast, no change was observed in mature unthinned and thinned forest. Wildfire decreased O-horizon Hg by >88% across all forest structures and decreased mineral-soil (0 to 66 mm depth) Hg by 50% in thinned forest and clear-cut. The wildfire-associated soil Hg loss was positively related to the amount of surface fine wood that burned during the fire, the proportion of area that burned at >700 degrees C, fire severity as indicated by tree mortality, and soil C loss. Loss of soil Hg due to the 200 000 ha wildfire was more than four times the annual atmospheric Hg emissions from human activities in Oregon. C1 [Homann, Peter S.] Western Washington Univ, Huxley Coll Environm, Bellingham, WA 98225 USA. [Darbyshire, Robyn L.] USDA Forest Serv, Pacific NW Reg, Portland, OR 97204 USA. [Bormann, Bernard T.] Univ Washington, Coll Environm, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Morrissette, Brett A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. RP Homann, PS (reprint author), Western Washington Univ, Huxley Coll Environm, Bellingham, WA 98225 USA. EM Peter.Homann@wwu.edu FU Joint Fire Sciences Program, Western Washington University [03-2-3-09, 10-1-10-18]; National Commission for Science on Sustainable Forestry [C4] FX This paper is a contribution of the USDA Forest Service, Pacific Northwest Research Station's Long-Term Ecosystem Productivity Program. Support for pre- and posttreatment, and postwildfire sampling and analysis came from the Research Station, the US Environmental Protection Agency, Environmental Research Laboratory, Corvallis, Oregon (Interagency Agreement DW 12936179), the Joint Fire Sciences Program (Grants 03-2-3-09 and 10-1-10-18), Western Washington University, the National Commission for Science on Sustainable Forestry (Grant C4), and the Rogue River-Siskiyou National Forest. We acknowledge the hard work of many individuals, including former LTEP experiment leaders Susan Little and Mike Amaranthus; agreement leads from Oregon State University, Kermit Cromack Jr. and Mark Harmon. This work would not have happened without the professional field and laboratory assistants from past years: Tom Bell, Aurore Chauvry, Matt Cowall, Colin Edgar, Laura Fabrey Stevens, Nate France, Nick Leahy, Kristina Muscutt, Suzanne Remillard, Vannessa Spini, Chris Stevens, Kyle Swanson, and Dave Woodruff; and from recent years: Amy Barnhart, Dylan Burgess, Nick Daniel, Martyn Davies, Emma Garner, Tim Martin, Kylie Meyer, and Alex Van Loo. We thank Julia Denning for preliminary study of soil mercury and Prof. Paul Gremillion for conducting mercury analyses at the Environmental Mercury Laboratory, Northern Arizona University. NR 70 TC 1 Z9 1 U1 7 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD NOV 3 PY 2015 VL 49 IS 21 BP 12714 EP 12722 DI 10.1021/acs.est.5b03355 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CV6BI UT WOS:000364355300013 PM 26485585 ER PT J AU Hufbauer, RA Szucs, M Kasyon, E Youngberg, C Koontz, MJ Richards, C Tuff, T Melbourne, BA AF Hufbauer, Ruth A. Szucs, Marianna Kasyon, Emily Youngberg, Courtney Koontz, Michael J. Richards, Christopher Tuff, Ty Melbourne, Brett A. TI Reply to Wootton and Pfister: The search for general context should include synthesis with laboratory model systems SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Letter ID EXTINCTION C1 [Hufbauer, Ruth A.; Szucs, Marianna; Kasyon, Emily; Youngberg, Courtney; Koontz, Michael J.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Hufbauer, Ruth A.; Koontz, Michael J.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Koontz, Michael J.] Univ Calif Davis, Grad Grp Ecol, Davis, CA 95616 USA. [Richards, Christopher] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Tuff, Ty; Melbourne, Brett A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. RP Hufbauer, RA (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM ruth.hufbauer@colostate.edu OI Koontz, Michael/0000-0002-8276-210X NR 5 TC 1 Z9 1 U1 4 U2 14 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD NOV 3 PY 2015 VL 112 IS 44 BP E5904 EP E5904 DI 10.1073/pnas.1517210112 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CV3NC UT WOS:000364164900002 PM 26483510 ER PT J AU Castelli, KR Barreto, MG Francesconi, W Dalla Valle, L Mondelli, G Abilio, FM da Silva, AM AF Castelli, Karen Regina Barreto, Mariana Gregorio Francesconi, Wendy Dalla Valle, Leandro Mondelli, Giulliana Abilio, Fernanda Maria da Silva, Alexandre Marco TI Analysis of effectiveness of three forest interventionist techniques and proposal of a new and integrated model of forest restoration SO ENVIRONMENTAL TECHNOLOGY LA English DT Article DE environmental indicator; environmental assessment; forest ecology; forest restoration technology; interventionist practices ID ECOLOGICAL RESTORATION; ATLANTIC FOREST; SEED DISPERSAL; SOIL; BIODIVERSITY; NUCLEATION; PLANT; PLANTATIONS; VEGETATION; BRAZIL AB We assessed the efficacy of three different forest intervention techniques, in terms of phytosociological and edaphic responses, that were implemented in 2007. In a farm where trees are planted and managed for cellulose production as well as set aside for environmental conservation, four stands were analysed: three of them were considered degraded and were managed using different intervention techniques (transposition, perch, and abandonment), and a fourth stand comprising pristine vegetation was considered a control (reference). Floristic and phytosociology data were collected in three 10x10m plots established in each stand. Also, a total of 48 soil samples were collected to analyse physical and chemical attributes of the topsoil for the different stands. In terms of biodiversity, all the treatments showed significantly lower values when compared to the reference area. However, the soils in all the treatment and reference stands are similar in terms of physical and chemical attributes. Taking into account the specificities of each restoration technique, we verified that the integrated use of a set of management practices, constituted by the (1) abandonment of the area and (2) following a selective killing of the eucalyptus, is the most suitable and promising model to provide fast and effective restoration in terms of environmental indicators. C1 [Castelli, Karen Regina; Barreto, Mariana Gregorio; Dalla Valle, Leandro; da Silva, Alexandre Marco] Sao Paulo State Univ, Dept Environm Engn, BR-18087180 Sorocaba, SP, Brazil. [Francesconi, Wendy] Purdue Univ, USDA ARS, NSERL, W Lafayette, IN 47907 USA. [Mondelli, Giulliana] Fed Univ ABC UFABC, Engn Modelling & Appl Social Sci Ctr CECS, BR-09210580 Santo Andre, SP, Brazil. [Abilio, Fernanda Maria] ECTX SA, BR-13323010 Salto, SP, Brazil. RP da Silva, AM (reprint author), Sao Paulo State Univ, Dept Environm Engn, Campus Sorocaba 511,Tres Marco Ave, BR-18087180 Sorocaba, SP, Brazil. EM amsilva@sorocaba.unesp.br RI Mondelli, Giulliana/F-6249-2014 FU Instituto de Pesquisas Tecnologicas do Estado de Sao Paulo (IPT); Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior (CAPES) FX The authors extend thanks to International Eucatex Corporation, for allowing the development of the project, Instituto de Pesquisas Tecnologicas do Estado de Sao Paulo (IPT) and Coordenacao de Aperfeicoamento de Pessoal de Ensino Superior (CAPES) for scholarships provided for students. The IPT also provided the laboratory for some analyses of soil physical attributes. NR 34 TC 0 Z9 0 U1 6 U2 41 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0959-3330 EI 1479-487X J9 ENVIRON TECHNOL JI Environ. Technol. PD NOV 2 PY 2015 VL 36 IS 21 BP 2712 EP 2723 DI 10.1080/09593330.2015.1043957 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CR9UW UT WOS:000361704000004 PM 25970012 ER PT J AU Reid, KF Kowaleski, CM Laussen, J Kirn, DR Hickey, C Curtatone, JA Fielding, R AF Reid, K. F. Kowaleski, C. M. Laussen, J. Kirn, D. R. Hickey, C. Curtatone, J. A. Fielding, R. TI TRANSLATING EXERCISE TO PRESERVE MOBILITY IN OLDER ADULTS: FROM CLINICAL TRIALS TO THE COMMUNITY SO GERONTOLOGIST LA English DT Meeting Abstract C1 [Reid, K. F.; Laussen, J.; Kirn, D. R.; Fielding, R.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Kowaleski, C. M.; Hickey, C.] City Somerville Council Aging, Holland St Senior Ctr, Somerville, MA USA. [Curtatone, J. A.] City Somerville, Somerville, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0016-9013 EI 1758-5341 J9 GERONTOLOGIST JI Gerontologist PD NOV PY 2015 VL 55 SU 2 BP 322 EP 322 PG 1 WC Gerontology SC Geriatrics & Gerontology GA DJ5BV UT WOS:000374222701624 ER PT J AU Kirn, DR Reid, KF Hau, C Phillips, E Fielding, R AF Kirn, D. R. Reid, K. F. Hau, C. Phillips, E. Fielding, R. TI WHAT IS A CLINICALLY MEANINGFUL CHANGE IN LOWER EXTREMITY POWER FOR MOBILITY-LIMITED OLDER ADULTS? SO GERONTOLOGIST LA English DT Meeting Abstract C1 [Kirn, D. R.; Reid, K. F.; Hau, C.; Phillips, E.; Fielding, R.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Phillips, E.] Harvard Univ, Sch Med, Dept Phys Med & Rehabil, Boston, MA USA. [Phillips, E.] Joslin Diabet Ctr, Inst Lifestyle Med, Boston, MA 02215 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0016-9013 EI 1758-5341 J9 GERONTOLOGIST JI Gerontologist PD NOV PY 2015 VL 55 SU 2 BP 734 EP 734 PG 1 WC Gerontology SC Geriatrics & Gerontology GA DJ5BV UT WOS:000374222703440 ER PT J AU Lu, HS Crow, WT Zhu, YH Yu, ZB Sun, JH AF Lu, Haishen Crow, Wade T. Zhu, Yonghua Yu, Zhongbo Sun, Jinhui TI The Impact of Assumed Error Variances on Surface Soil Moisture and Snow Depth Hydrologic Data Assimilation SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Data assimilation (DA); error variance; hydrology model; soil moisture (SM); snow depth (SD) ID ENSEMBLE KALMAN FILTER; STATE-PARAMETER ESTIMATION; IN-SITU OBSERVATIONS; REMOTE-SENSING DATA; COVERED AREA; MODEL; PREDICTION; SCALE; SCATTEROMETER; SIMULATIONS AB Accurate knowledge of antecedent soil moisture (SM) and snow depth (SD) conditions is often important for obtaining reliable hydrological simulations of stream flow. Data assimilation (DA) methods can be used to integrate remotely sensed (RS) SM and SD retrievals into a hydrology model and improve such simulations. In this paper, we examine the impact of assumed model and observation error variance on stream flow estimates obtained by assimilating RS SM and SD data into a lumped hydrological model. The analysis is based on both synthetic and real DA experiments conducted within the Tuotuo River watershed at the headwaters of the Yangtze River. Synthetic experiments demonstrate that, when the true model error variance is small, DA is more sensitive to the overestimation of the error variance than to its underestimation. Conversely, if the true variance is large, DA is sensitive to the assumed model error variance but not the underestimation of the observation error variance. Given this sensitivity, the maximum a posteriori (MAP) estimation method is applied to accurately estimate model and observation error variances. In general, MAP is able to identify model and observation error parameters associated with an accurate stream flow analysis. However, its utility is somewhat limited by equifinality with regard to observation error statistics. C1 [Lu, Haishen; Zhu, Yonghua; Yu, Zhongbo] Hohai Univ, Coll Hydrol & Water Resources, State Key Lab Hydrol Water Resources & Hydraul En, Nanjing 210098, Jiangsu, Peoples R China. [Lu, Haishen; Crow, Wade T.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Sun, Jinhui] China Water Int Engn Consulting Co Ltd, Beijing, Peoples R China. RP Lu, HS (reprint author), Hohai Univ, Coll Hydrol & Water Resources, State Key Lab Hydrol Water Resources & Hydraul En, Nanjing 210098, Jiangsu, Peoples R China. EM wade.crow@ars.usda.gov FU National Basic Research Program of China [2013CBA01806]; NNSF [41371049, 41571015, 41323001, 51190091]; State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering [20145027312]; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography; PAPD FX This work was supported in part by the National Basic Research Program of China under Grant 2013CBA01806, in part by NNSF under Grant 41371049, Grant 41571015, Grant 41323001, and Grant 51190091, in part by the State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering under Grant 20145027312, and in part by the State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography; PAPD. NR 54 TC 3 Z9 3 U1 0 U2 3 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD NOV PY 2015 VL 8 IS 11 SI SI BP 5116 EP 5129 DI 10.1109/JSTARS.2015.2487740 PG 14 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA DD4OE UT WOS:000369901600012 ER PT J AU Heller, WP Kissinger, KR Matsumoto, TK Keith, LM AF Heller, Wade P. Kissinger, Karma R. Matsumoto, Tracie K. Keith, Lisa M. TI Utilization of papaya waste and oil production by Chlorella protothecoides SO ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS LA English DT Article DE Chlorella protothecoides; Lipids; Biofuels; Agricultural waste; Zero-waste ID BIODIESEL PRODUCTION; MICROALGAE; GENOME; GROWTH; FERMENTATION; ACCUMULATION; CULTIVATION; ADAPTATION; MOLASSES; QUALITY AB Microalgae derived oils have outstanding potential for use in biodiesel production. Chlorella protothecoides has been shown to accumulate lipids up to 60% of its cellular dry weight with glucose supplementation under heterotrophic growth conditions. To reduce production costs, alternative carbon feedstocks have been evaluated and show promise as low-cost alternatives. Regional agricultural residues, such as off-quality fruits and vegetables that cannot be used as human food or animal feed, as well as inedible plant byproducts, represent an abundant and underutilized resource as a feedstock for supporting microbial production of biofuels. Here, we present the finding that C. protothecoides isolates are capable of robust cell growth and oil production in growth medium comprised of pH-adjusted puree of culled, waste papaya fruit without any additional growth supplements. Optimization of culture medium and growth conditions was used for lab-scale strain characterization and demonstration of the potential for scale-up to an industrial bioprocess. The most rapid cell doubling time observed was 6.6 h and the maximum oil production rate observed was 1.9 g/l/day. (C) 2015 Elsevier B. V. All rights reserved. C1 [Heller, Wade P.] Univ Hawaii, Coll Agr Forestry & Nat Resource Management, 200 W Kawili St, Hilo, HI 96720 USA. [Kissinger, Karma R.; Matsumoto, Tracie K.; Keith, Lisa M.] ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Trop Plant Genet Resources & Dis Res Unit, Hilo, HI 96720 USA. RP Keith, LM (reprint author), ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Trop Plant Genet Resources & Dis Res Unit, Hilo, HI 96720 USA. EM Lisa.Keith@ars.usda.gov FU Hawaii Department of Agriculture; Agribusiness Development Corporation; Office of Naval Research FX This project was funded by the Hawaii Department of Agriculture, Agribusiness Development Corporation and the Office of Naval Research. The authors thank Dr. Dennis Gonsalves for his vision and scientific discussion on the use of feedstock from Hawaii and Hawaiian Fresh Products, Inc. for the papaya culls. NR 27 TC 0 Z9 0 U1 6 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-9264 J9 ALGAL RES JI Algal Res. PD NOV PY 2015 VL 12 BP 156 EP 160 DI 10.1016/j.algal.2015.08.013 PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DB9IL UT WOS:000368829500020 ER PT J AU Bhima, AN Park, JH Cho, M Yi, YJ Oh, SG Park, YJ Lovanh, N Kamala-Kannan, S Oh, BT AF Bhima, Arun Naidu Park, Jung-Hee Cho, Min Yi, Young-Joo Oh, Sae-Gang Park, Yool-Jin Lovanh, Nanh Kamala-Kannan, Seralathan Oh, Byung-Taek TI Simultaneous utilization of soju industrial waste for silica production and its residue ash as effective cationic dye adsorbent SO E-POLYMERS LA English DT Article DE adsorption; isotherms; kinetic models; methylene blue; nanosilica; soju industrial waste ID DILUTE AQUEOUS-SOLUTIONS; LOW-COST ADSORBENT; RICE HULL ASH; METHYLENE-BLUE; BASIC DYE; ACTIVATED CARBON; AGRICULTURAL WASTE; BATCH ADSORPTION; REMOVAL; EQUILIBRIUM AB Soju industrial waste is an important biomass resource. The present study aimed to utilize soju industrial waste for silica extraction, and residual ash (RA) as a low cost adsorbent for the removal of methylene blue (MB) from aqueous solution. A high percentage of pure amorphous nanosilica was obtained from soju industrial waste ash by the acid dissolution-precipitation process. The synthesized nanosilica and the RA were characterized well using various techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), and field-emission electron microscopy with energy dispersive spectroscopy (EDS). The amorphous nature of nanosilica and its 99% purity were confirmed by XRD and EDS profiles, respectively. Transmission electron microscopy (TEM) micrographs show the nano range (14-18 nm) of synthesized silica. The adsorption capacity of RA was evaluated as a function of initial dye concentration, pH, and contact time. The sorption equilibrium data were modeled with isotherms; the Langmuir isotherm model fits well with maximum monolayer adsorption capacity of 232.5 mg/g at 30 degrees C. The adsorption kinetics was best fitted with the pseudo-second-order model, suggesting that chemisorption plays a significant role in the adsorption process. The results showed that soju industrial waste is a potential waste for silica extraction and that its byproducts are effective adsorbents. C1 [Bhima, Arun Naidu; Park, Jung-Hee; Cho, Min; Yi, Young-Joo; Kamala-Kannan, Seralathan; Oh, Byung-Taek] Chonbuk Natl Univ, Adv Inst Environm & Biosci, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, South Korea. [Oh, Sae-Gang] Mine Reclamat Corp, Seoul 110727, South Korea. [Park, Yool-Jin] Chonbuk Natl Univ, Dept Ecol Landscape Architecture Design, Coll Environm & Bioresource Sci, Iksan 570752, South Korea. [Kamala-Kannan, Seralathan] ARS, USDA, AWMRU, Bowling Green, KY 42104 USA. RP Kamala-Kannan, S (reprint author), Chonbuk Natl Univ, Adv Inst Environm & Biosci, Div Biotechnol, Coll Environm & Bioresource Sci, Iksan 570752, South Korea. EM kannan@jbnu.ac.kr; btoh@jbnu.ac.kr FU Korean National Research Foundation (Korean Ministry of Education, Science and Technology) [NRF-2011-35B-D00020] FX This research was supported by the Korean National Research Foundation (Korean Ministry of Education, Science and Technology, Award NRF-2011-35B-D00020). NR 55 TC 0 Z9 0 U1 2 U2 3 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 1618-7229 J9 E-POLYMERS JI e-Polymers PD NOV PY 2015 VL 15 IS 6 BP 427 EP 437 DI 10.1515/epoly-2015-0108 PG 11 WC Polymer Science SC Polymer Science GA DC0KJ UT WOS:000368905600008 ER PT J AU DeGraff, JV Cannon, SH Gartner, JE AF DeGraff, Jerome V. Cannon, Susan H. Gartner, Joseph E. TI The Timing of Susceptibility to Post-Fire Debris Flows in the Western United States SO ENVIRONMENTAL & ENGINEERING GEOSCIENCE LA English DT Article DE Debris Flow; Timing; Wildfire; Forest Cover; Recovery ID FIRE REGIMES; SOUTHERN CALIFORNIA; BURNED AREAS; NEW-MEXICO; WILDFIRE; USA; MOUNTAIN; COLORADO; FLOODS; GENERATION AB Watersheds recently burned by wildfires can be susceptible to debris flow, although little is known about how long this susceptibility persists and how it changes over time. We use a compilation of 75 debris-flow response and fire-ignition dates, vegetation and bedrock class, rainfall regime, and initiation process from throughout the western United States to address these issues. The great majority (85 percent) of debris flows occurred within the first 12 months following wildfire, with 71 percent occurring within the first 6 months. Seven percent of the debris flows occurred between 1 and 1.5 years after a fire, or during the second rainy season to impact an area. Within the first 1.5 years following fires, all but one of the debris flows initiated through runoff-dominated processes, and debris flows occurred in similar proportions in forested and non-forested landscapes. Underlying geologic materials affected how long debris-flow activity persisted, and the timing of debris flows varied within different rainfall regimes. A second, later period of increased debris flow susceptibility between 2.2 and 10 years after fires is indicated by the remaining 8 percent of events, which occurred primarily in forested terrains and initiated largely through landslide processes. The short time period between fire and debris-flow response within the first 1.5 years after ignition and the longer-term response between 2.2 and 10 years after fire demonstrate the necessity of both rapid and long-term reactions by land managers and emergency-response agencies to mitigate hazards from debris flows from recently burned areas in the western United States. C1 [DeGraff, Jerome V.] US Forest Serv, USDA, 1600 Tollhouse Rd, Clovis, CA 93611 USA. [Cannon, Susan H.] US Geol Survey, Denver, CO 80225 USA. [Gartner, Joseph E.] BGC Engn, Golden, CO 80401 USA. RP DeGraff, JV (reprint author), US Forest Serv, USDA, 1600 Tollhouse Rd, Clovis, CA 93611 USA. NR 67 TC 2 Z9 2 U1 4 U2 10 PU GEOLOGICAL SOC AMER, INC PI BOULDER PA PO BOX 9140, BOULDER, CO 80301-9140 USA SN 1078-7275 EI 1558-9161 J9 ENVIRON ENG GEOSCI JI Environ. Eng. Geosci. PD NOV PY 2015 VL 21 IS 4 BP 277 EP 292 PG 16 WC Engineering, Environmental; Engineering, Geological; Geosciences, Multidisciplinary SC Engineering; Geology GA DC0GT UT WOS:000368895900002 ER PT J AU Fuss, CB Driscoll, CT Campbell, JL AF Fuss, Colin B. Driscoll, Charles T. Campbell, John L. TI Recovery from chronic and snowmelt acidification: Long-term trends in stream and soil water chemistry at the Hubbard Brook Experimental Forest, New Hampshire, USA SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article; Proceedings Paper CT 2nd International Conference on Arctic Research Planning CY NOV 10-12, 2005 CL Copenhagen, DENMARK ID DISSOLVED ORGANIC-CARBON; NORTHERN HARDWOOD FOREST; NORTHEASTERN UNITED-STATES; EPISODIC ACIDIFICATION; ACIDIC DEPOSITION; NEW-YORK; SURFACE WATERS; SUGAR MAPLE; ADIRONDACK MOUNTAINS; LANDSCAPE POSITION AB Atmospheric acid deposition of sulfate and nitrate has declined markedly in the northeastern United States due to emissions controls. We investigated long-term trends in soil water (1984-2011) and stream water (1982-2011) chemistry along an elevation gradient of a forested watershed to evaluate the progress of recovery of drainage waters from acidic deposition at the Hubbard Brook Experimental Forest in the White Mountains of New Hampshire, USA. We found slowed losses of base cations from soil and decreased mobilization of dissolved inorganic aluminum. Stream water pH at the watershed outlet increased at a rate of 0.01 units yr(-1), and the acid neutralizing capacity (ANC) gained 0.88 mu eq L-1 yr(-1). Dissolved organic carbon generally decreased in stream water and soil solutions, contrary to trends observed at many North American and European sites. We compared whole-year hydrochemical trends with those during snowmelt, which is the highest-flow and lowest ANC period of the year, indicative of episodic acidification. Stream water during snowmelt had long-term trends of increasing ANC and pH at a rate very similar to the whole-year record, with closely related steady decreases in sulfate. A more rapid decline in stream water nitrate during snowmelt compared with the whole-year trend may be due, in part, to the marked decrease in atmospheric nitrate deposition during the last decade. The similarity between the whole-year trends and those of the snowmelt period is an important finding that demonstrates a consistency between recovery from chronic acidification during base flow and abatement of snowmelt acidification. C1 [Fuss, Colin B.; Driscoll, Charles T.] Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. [Fuss, Colin B.] Cary Inst Ecosyst Studies, Millbrook, NY USA. [Campbell, John L.] US Forest Serv, Northern Res Stn, Durham, NH USA. RP Fuss, CB (reprint author), Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. EM fussc@caryinstitute.org OI Campbell, John/0000-0003-4956-1696; Driscoll, Charles/0000-0003-2692-2890 FU National Science Foundation [DEB 0949664, DEB 1114804]; Department of Civil and Environmental Engineering at Syracuse University; Wen-Hsiung and Kuan-Ming Li Fellowship from Department of Civil and Environmental Engineering, Syracuse University FX We thank K. Driscoll for the assistance with statistical analysis and M. Montesdeoca and M. Rice for the quality control of laboratory data. C. Johnson, L. Lautz, M. Green, P. Groffman, D. Chandler and several anonymous reviewers provided many useful comments. The HBEF is administered by the U.S. Department of Agriculture Forest Service, Northern Forest Research Station, Newtown Square, PA. This work is a contribution to the Hubbard Brook Ecosystem Study. Hubbard Brook is a National Science Foundation supported Long-Term Ecological Research (LTER) site. The data used in this study are available through the data repository of the Hubbard Brook Ecosystem Study at www.hubbardbrook.org. Support for this project was provided by the National Science Foundation (grants DEB 0949664-Ecosystem Studies and DEB 1114804-Long-Term Ecological Research) and by the Department of Civil and Environmental Engineering at Syracuse University. C. Fuss was supported by the Wen-Hsiung and Kuan-Ming Li Fellowship from the Department of Civil and Environmental Engineering, Syracuse University. NR 95 TC 6 Z9 6 U1 7 U2 22 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD NOV PY 2015 VL 120 IS 11 BP 2360 EP 2374 DI 10.1002/2015JG003063 PG 15 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DC0LM UT WOS:000368908700017 ER PT J AU Papanicolaou, AN Wacha, KM Abban, BK Wilson, CG Hatfield, JL Stanier, CO Filley, TR AF Papanicolaou, A. N. (Thanos) Wacha, Kenneth M. Abban, Benjamin K. Wilson, Christopher G. Hatfield, Jerry L. Stanier, Charles O. Filley, Timothy R. TI From soilscapes to landscapes: A landscape-oriented approach to simulate soil organic carbon dynamics in intensively managed landscapes SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article; Proceedings Paper CT 2nd International Conference on Arctic Research Planning CY NOV 10-12, 2005 CL Copenhagen, DENMARK ID SEDIMENT TRANSPORT CAPACITY; WATER EROSION; HYDRAULIC CONDUCTIVITY; MATTER DYNAMICS; WEPP MODEL; SPATIAL VARIABILITY; MICROBIAL BIOMASS; ENRICHMENT RATIO; STABLE-ISOTOPES; TILLAGE EROSION AB Most available biogeochemical models focus within a soil profile and cannot adequately resolve contributions of the lighter size fractions of organic rich soils for enrichment ratio (ER) estimates, thereby causing unintended errors in soil organic carbon (SOC) storage predictions. These models set ER as constant, usually equal to unity. The goal of this study is to provide spatiotemporal predictions of SOC stocks at the hillslope scale that account for the selective entrainment and deposition of lighter size fractions. It is hypothesized herein that ER values may vary depending on hillslope location, Land Use/Land Cover (LULC) conditions, and magnitude of the hydrologic event. An ER module interlinked with two established models, CENTURY and Watershed Erosion Prediction Project, is developed that considers the effects of changing runoff coefficients, bare soil coverage, tillage depth, fertilization, and soil roughness on SOC redistribution and storage. In this study, a representative hillslope is partitioned into two control volumes (CVs): a net erosional upslope zone and a net depositional downslope zone. We first estimate ER values for both CVs I and II for different hydrologic and LULC conditions. Second, using the improved ER estimates for the two CVs, we evaluate the effects that management practices have on SOC redistribution during different crop rotations. Overall, LULC promoting less runoff generally yielded higher ER values, which ranged between 0.97 and 3.25. Eroded soils in the upland CV were up to 4% more enriched in SOC than eroded soils in the downslope CV due to larger interrill contributions, which were found to be of equal importance to rill contributions. The chronosequence in SOC storage for the erosional zone revealed that conservation tillage and enhanced crop yields begun in the 1980s reversed the downward trend in SOC losses, causing nearly 26% of the lost SOC to be regained. C1 [Papanicolaou, A. N. (Thanos); Abban, Benjamin K.; Wilson, Christopher G.] Univ Tennessee, Dept Civil & Environm Engn, Hydraul & Sedimentat Lab, Knoxville, TN USA. [Wacha, Kenneth M.] Univ Iowa, Dept Civil & Environm Engn, IIHR, Hydrosci & Engn, Iowa City, IA 52242 USA. [Hatfield, Jerry L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Stanier, Charles O.] Univ Iowa, Dept Chem & Biochem Engn, IIHR, Hydrosci & Engn, Iowa City, IA 52242 USA. [Filley, Timothy R.] Purdue Univ, Dep Earth Atmospher & Planetary Sci, W Lafayette, IN 47907 USA. RP Papanicolaou, AN (reprint author), Univ Tennessee, Dept Civil & Environm Engn, Hydraul & Sedimentat Lab, Knoxville, TN USA. EM tpapanic@utk.edu RI Stanier, Charles/D-4307-2016; OI Stanier, Charles/0000-0001-9924-0853; Abban, Benjamin/0000-0001-9507-5440 FU National Science Foundation [EAR1331906]; NASA EPSCoR [NNX10AN28A]; Iowa Space Grant Consortium [NNX10AK63H]; Leopold Center for Sustainable Agriculture grant [XP2010-03]; Graduate Assistance in Areas of National Need (GAANN) fellowship; University of Iowa NSF IGERT program, Geoinformatics for Environmental and Energy Modeling and Prediction FX The modeling component of this research was supported by the National Science Foundation grant EAR1331906 for the Critical Zone Observatory for intensively managed landscapes (IML-CZO), a multiinstitutional collaborative effort. Additionally, this research was partially supported by the NASA EPSCoR Program (grant NNX10AN28A) and the Iowa Space Grant Consortium (grant NNX10AK63H) as well as the Leopold Center for Sustainable Agriculture grant XP2010-03 where data for model calibration and validation have been collected. The second author was partially supported by a Graduate Assistance in Areas of National Need (GAANN) fellowship grant and the University of Iowa NSF IGERT program, Geoinformatics for Environmental and Energy Modeling and Prediction. The authors would like to recognize the help of Cindy Keough from Natural Resources Ecology Laboratory-Colorado State University for technical advice in coupling the CENTURY model with WEPP. As always the authors would like to acknowledge the long-term partnership with the ARS lab at West Lafayette and Dennis Flanagan for working with different versions of the WEPP model. The data of this research are available to the interested reader upon written request to the first author. NR 175 TC 2 Z9 2 U1 4 U2 15 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD NOV PY 2015 VL 120 IS 11 BP 2375 EP 2401 DI 10.1002/2015JG003078 PG 27 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DC0LM UT WOS:000368908700018 ER PT J AU White, JW Alagarswamy, G Ottman, MJ Porter, CH Singh, U Hoogenboom, G AF White, J. W. Alagarswamy, G. Ottman, M. J. Porter, C. H. Singh, U. Hoogenboom, G. TI An Overview of CERES-Sorghum as Implemented in the Cropping System Model Version 4.5 SO AGRONOMY JOURNAL LA English DT Article ID BICOLOR L. MOENCH; ROOT-GROWTH; CARBON-DIOXIDE; CLIMATE-CHANGE; WATER-USE; YIELD; PHOTOSYNTHESIS; TEMPERATURE; SIMULATION; DROUGHT AB Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important grain crop globally. It stands out for its diversity of plant types, end-uses, and roles in cropping systems. This diversity presents opportunities but also complicates evaluation of production options, especially under climate uncertainty. Ecophysiological models can dissect interacting effects of plant genotypes, crop management, and environment. We describe the sorghum module of the Cropping System Model (CSM) as implemented in the Decision Support System for Agrotechnology Transfer (DSSAT) to illustrate potential applications and suggest areas for model improvement. Crop growth is simulated based on radiation use efficiency. Development responds to temperature and photoperiod. Partitioning rules vary with growth stages, respecting mass balance and maintaining functional equilibrium between roots and shoots. Routines for climate, soil, crop management, and model controls are shared with other crops in CSM. Modeled responses for eight real-world and hypothetical cases are presented. These include growth under well-managed conditions, responses to row-spacing, population, sowing date, irrigation, defoliation, and increased atmospheric carbon dioxide concentration ([CO2]), and a long-term sorghum and winter wheat (Triticum aestivum L.) rotation. Among traits and experiments considered, model accuracy was high for phenology (r(2) = 0.96, P < 0.01 for anthesis and r(2) = 0.91, P < 0.01 for maturity), moderate for grain yields (r(2) values from 0.30 to 0.52, P < 0.01), depending on the simulated experiments, and low for unit grain weight (r(2) = 0.02, not significant, NS) and leaf area index for forage sorghum (r(2) = 0.18, NS). C1 [White, J. W.] USDA ARS, Maricopa, AZ 85138 USA. [Alagarswamy, G.] Michigan State Univ, Ctr Global Change & Earth Observ, E Lansing, MI 48824 USA. [Ottman, M. J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Porter, C. H.] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Singh, U.] Int Fertilizer Dev Ctr, Muscle Shoals, AL 35661 USA. [Hoogenboom, G.] Washington State Univ, AgWeatherNet Program, Prosser, WA 99350 USA. RP White, JW (reprint author), USDA ARS, Arid Land,21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM jeffrey.White@ars.usda.gov RI KUMAR, ARVIND/D-7733-2016 OI KUMAR, ARVIND/0000-0001-8818-6222 NR 56 TC 2 Z9 2 U1 3 U2 8 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 1987 EP 2002 DI 10.2134/agronj15.0102 PG 16 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900004 ER PT J AU Liebig, MA Hendrickson, JR Archer, DW Schnner, MA Nichols, KA Tanaka, DL AF Liebig, M. A. Hendrickson, J. R. Archer, D. W. Schnner, M. A. Nichols, K. A. Tanaka, D. L. TI Short-Term Soil Responses to Late-Seeded Cover Crops in a Semi-Arid Environment SO AGRONOMY JOURNAL LA English DT Article ID ECOSYSTEM SERVICES; CROPPING SYSTEM; QUALITY; PRINCIPLES; MIXTURES; BENEFITS; TILLAGE; CORN AB Cover crops can expand ecosystem services, though sound management recommendations for their use within semiarid cropping systems is currently constrained by a lack of information. This study was conducted to determine agroecosystem responses to late-summer seeded cover crops under no-till management, with particular emphasis on soil attributes. Short-term effects of late-summer seeded cover crops on soil water, available N, nearsurface soil quality, and residue cover were investigated during three consecutive years on the Area IV Soil Conservation Districts Research Farm near Mandan, ND. Mean aboveground cover crop biomass was highly variable across years (1430, 96, and 937 kg ha(-1) in 2008, 2009, and 2010, respectively), and was strongly affected by precipitation received within 14 d following cover crop seeding. During years with appreciable biomass production (2008 and 2010), cover crops significantly reduced available N in the 0.9-m depth the following spring (P = 0.0291 and 0.0464, respectively). Cover crop effects on soil water were subtle, and no differences in soil water were found between cover crop treatments and a no cover crop control before seeding cash crops the following spring. Late-summer seeded cover crops did not affect near-surface soil properties or soil coverage by residue. Soil responses to late-summer seeded cover crops did not differ between cover crop mixtures and monocultures. Late-summer seeded cover crops may enhance ecosystem services provided by semiarid cropping systems through biomass production and N conservation, though achieving these benefits in a consistent manner appears dependent on timely precipitation following cover crop seeding. C1 [Liebig, M. A.; Hendrickson, J. R.; Archer, D. W.; Tanaka, D. L.] USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Schnner, M. A.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Nichols, K. A.] Rodale Inst, Kutztown, PA 19530 USA. RP Liebig, MA (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM mark.liebig@ars.usda.gov NR 36 TC 2 Z9 2 U1 5 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2011 EP 2019 DI 10.2134/agronj15.0146 PG 9 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900006 ER PT J AU Thompson, LJ Ferguson, RB Kitchen, N Frazen, DW Mamo, M Yang, H Schepers, JS AF Thompson, L. J. Ferguson, R. B. Kitchen, N. Frazen, D. W. Mamo, M. Yang, H. Schepers, J. S. TI Model and Sensor-Based Recommendation Approaches for In-Season Nitrogen Management in Corn SO AGRONOMY JOURNAL LA English DT Article ID REFLECTANCE MEASUREMENTS; SYSTEMS SIMULATION; YIELD RESPONSE; USE EFFICIENCY; MAIZE; FERTILIZATION; FERTILITY; QUEFTS AB Nitrogen management for corn (Zea mays L.) may be improved by applying a portion of N in-season. This investigation was conducted to evaluate crop modeling (Maize-N) and active crop canopy sensing approaches for recommending in-season N fertilizer rates. These approaches were evaluated during 2012-2013 on 11 field sites, in Missouri, Nebraska, and North Dakota. Nitrogen management also included a no-N treatment (check) and a non-limiting N reference (all at planting). Nitrogen management treatments were assessed for two hybrids and at low and high seeding rates, arranged in a randomized complete block design. In 9 of 11 site-years, the sensor-based approach recommended lower in-season N rates than the model (collectively 59% less N), resulting in trends of higher partial factor productivity of nitrogen (PFPN) and higher agronomic efficiency (AE) than the model. However, yield was better protected by the model-based approach. In some situations, canopy sensing excelled at optimizing the N rate for localized conditions. With abnormally warm and moist soil conditions for the 2012 Nebraska sites and presumed high levels of inorganic N from mineralization, N application was appropriately reduced, resulting in no yield decrease and N savings compared to the non-limiting N reference. Depending on the site, both recommendation approaches were successful; a combination of model and sensor information may optimize in-season decision support for N recommendation. C1 [Thompson, L. J.] Univ Nebraska Lincoln, Southeast Res & Extens Ctr, 1071 CR G, Ithaca, NE 68033 USA. [Ferguson, R. B.; Mamo, M.; Yang, H.] Univ Nebraska Lincoln, Argon & Hort, Lincoln, NE 68583 USA. [Kitchen, N.] USDA, Columbia, MO 65211 USA. [Frazen, D. W.] N Dakota State Univ, Soil Sci, Fargo, ND 58108 USA. [Schepers, J. S.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Schepers, J. S.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RP Thompson, LJ (reprint author), Univ Nebraska Lincoln, Southeast Res & Extens Ctr, 1071 CR G, Ithaca, NE 68033 USA. EM laura.thompson@unl.edu NR 37 TC 1 Z9 1 U1 1 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2020 EP 2030 DI 10.2134/agronj15.0116 PG 11 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900007 ER PT J AU Nielsen, DC Lyon, DJ Hergert, GW Higgins, RK Holman, JD AF Nielsen, David C. Lyon, Drew J. Hergert, Gary W. Higgins, Robert K. Holman, Johnathan D. TI Cover Crop Biomass Production and Water Use in the Central Great Plains SO AGRONOMY JOURNAL LA English DT Article ID USE EFFICIENCY; LIMITED ENVIRONMENTS; WIND EROSION; BARLEY-PEA; WHEAT; YIELD; MANAGEMENT; MIXTURES; SYSTEM; FALLOW AB The water-limited environment of the semiarid Central Great Plains may not produce enough cover crop biomass to generate benefits associated with cover crop use in more humid regions. There have been reports that cover crops grown in mixtures produce more biomass with greater water use efficiency than single-species plantings. This study was conducted to determine differences in cover crop biomass production, water use efficiency, and residue cover between a mixture and single-species plantings. The study was conducted at Akron, CO, and Sidney, NE, during the 2012 and 2013 growing seasons under both rainfed and irrigated conditions. Water use, biomass, and residue cover were measured and water use efficiency was calculated for four single-species cover crops (flax [Linum usitatissimum L.], oat [Avena sativa L.], pea [Pisum sativum ssp. arvense L. Poir], rapeseed [Brassica napus L.]) and a 10-species mixture. The mixture did not produce greater biomass nor exhibit greater water use efficiency than the single-species plantings. The slope of the water-limited yield relationship was not significantly greater for the mixture than for single-species plantings. Water-limited yield relationship slopes were in the order of rapeseed < flax < pea < mixture < oat, which was the expected order based on previously published biomass productivity values generated from values of glucose conversion into carbohydrates, protein, or lipids. Residue cover was not generally greater from the mixture than from single-species plantings. The greater expense associated with a mixture is not justified unless a certain cover crop forage quality is required for grazing or haying. C1 [Nielsen, David C.] USDA ARS, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. [Lyon, Drew J.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Hergert, Gary W.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Higgins, Robert K.] Univ Nebraska, High Plains Ag Lab, Sidney, NE 69162 USA. [Holman, Johnathan D.] Southwest Res & Extens Ctr, Garden City, KS 67846 USA. RP Nielsen, DC (reprint author), USDA ARS, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. EM david.nielsen@ars.usda.gov RI Nielsen, David/A-8044-2009 OI Nielsen, David/0000-0002-8240-7183 NR 53 TC 2 Z9 2 U1 19 U2 70 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2047 EP 2058 DI 10.2134/agronj15.0186 PG 12 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900010 ER PT J AU Wilkens, S Weimer, PJ Lauer, JG AF Wilkens, Steve Weimer, Paul J. Lauer, Joseph G. TI The Effects of Hybrid Relative Maturity on Corn Stover for Ethanol Production and Biomass Composition SO AGRONOMY JOURNAL LA English DT Article ID ZEA-MAYS L.; FORAGE YIELD; MAIZE HYBRIDS; QUALITY; SILAGE; SACCHARIFICATION; TRAITS; GROWTH; FIBER; PARTS AB Full-season corn (Zea mays L.) hybrids take advantage of more of the growing season than shorter-season hybrids oft en leading to greater grain and biomass yield. Many agronomic experiments aimed at corn stover production have been performed at forage harvest rather than later when stover is normally harvested for biofuel measurements. The objective of this research was to evaluate the influence of hybrid relative maturity (days RM) on stover ethanol production, ruminant digestibility, and biomass composition. Hybrids selected were high-yielding commercial grain hybrids grown throughout Wisconsin and ranged from 85 to 115 d RM in 10 d RM increments during 2009, and in 5 d RM increments during 2010. Hybrids were harvested at physiological maturity or aft er a killing frost. Overall, stover and theoretical ethanol yields increased as RM increased at a linear rate of 0.211 Mg ha(-1) RM-1 and 67.1 L ha(-1) RM-1. Stover nutritional and biomass composition improved as RM increased, but yield variability was greater than nutritional and biomass compositional variability. Increasing ethanol yields will likely occur by increasing stover yields rather than by altering stover composition. Therefore, until price premiums for stover composition are made available to farmers for ethanol production, the adoption of full-season or longer maturing hybrids should be implemented for increased stover and ethanol yields. C1 [Wilkens, Steve; Lauer, Joseph G.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Weimer, Paul J.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Lauer, JG (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM jglauer@wisc.edu NR 44 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2303 EP 2311 DI 10.2134/agronj15.0123 PG 9 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900032 ER PT J AU Hartman, GL Bowen, CR Haudenshield, JS Fox, CM Cary, TR Diers, BW AF Hartman, G. L. Bowen, C. R. Haudenshield, J. S. Fox, C. M. Cary, T. R. Diers, B. W. TI Evaluation of Disease and Pest Damage on Soybean Cultivars Released from 1923 through 2008 under Field Conditions in Central Illinois SO AGRONOMY JOURNAL LA English DT Article ID HETERODERA-GLYCINES ICHINOHE; EAST-CENTRAL ILLINOIS; GENETIC GAIN; YIELD; IMPROVEMENT; RESISTANCE; VIRUS AB Diseases and pests of soybean [Glycine max (L.) Merr.] often reduce soybean yields. Targeted breeding that incorporates known genes for resistance and non-targeted breeding that eliminates susceptible plants in breeding populations reduces the impact of soybean pathogens and pests. Maturity group (MG) III soybean cultivars released from 1923 through 2008 were grown in three field environments to determine if disease and insect ratings were associated with year of cultivar release. Disease and pest ratings were evaluated on 40 soybean cultivars at one location (Urbana, IL) planted in two rotation treatments in 2010 and on 59 cultivars in two locations (Urbana and Arthur) in 2011. During the season, foliar disease symptoms and insect foliar feeding damage were recorded. At harvest maturity, stem diseases were assessed. In at least one environment, foliar incidence reached 100% for bacterial diseases, brown spot (Septoria glycines Hemmi), and insect foliar feeding damage and 100% incidence for anthracnose [Colletotrichum truncatum (Schwein.) Andrus & W.D. Moore], Cercospora stem blight (Cercospora kikuchii T. Matsumoto & Tomoy.), and charcoal rot [Macrophomina phaseolina (Tassi) Goid.] on stems for all cultivars. For the nine different disease and pest severity assessments in 2010, seven had a significant (P < 0.05) negative correlation to year of cultivar release indicating that cultivars more recently released had lower severity ratings than cultivars with older release dates. This study demonstrated that incidence and severity of diseases were less pronounced on more newly-released soybean cultivars, showing that decades of breeding has resulted in increased disease resistance in modern soybean cultivars. C1 [Hartman, G. L.; Bowen, C. R.; Haudenshield, J. S.] USDA ARS, 1101 W Peabody Dr, Urbana, IL 61801 USA. [Haudenshield, J. S.; Fox, C. M.; Cary, T. R.; Diers, B. W.] Univ Illinois, Dept Crop Sci, 1101 W Peabody Dr, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), USDA ARS, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU Pioneer Fellowship in Plant Breeding FX We would like to thank the Pioneer Fellowship in Plant Breeding and that of Ellen West for her contribution in data collection. NR 40 TC 2 Z9 2 U1 2 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2373 EP 2380 DI 10.2134/agronj15.0075 PG 8 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900040 ER PT J AU Mirsky, SB Wallace, JM Curran, WS Crockett, BC AF Mirsky, Steven B. Wallace, John M. Curran, William S. Crockett, Benjamin C. TI Hairy Vetch Seedbank Persistence and Implications for Cover Crop Management SO AGRONOMY JOURNAL LA English DT Article ID ORGANIC CORN PRODUCTION; WEED MANAGEMENT; SOIL DISTURBANCE; FARMING SYSTEMS; TILLAGE; EMERGENCE; GERMINATION; DEPTH; SEEDS; TEMPERATURE AB Hairy vetch (Vicia villosa Roth) is a fast-growing, winter-hardy annual legume that is well suited for summer annual grain rotations because it fixes N-2, reduces erosion, and suppresses weeds. Utilization of hairy vetch as a cover crop has been limited due to producer concerns regarding its potential to become a weed in subsequent crops. Hairy vetch weediness is attributed to its hard seed, a physical dormancy trait common to Vicia species. A 2-yr field experiment was conducted in Pennsylvania and Maryland to characterize the effects of mechanical scarification and burial depth on seedbank dynamics using two cultivars. Field and laboratory measurements were conducted to determine seedbank fate: seedling emergence, seedbank persistence, and seedbank loss. Hairy vetch emergence and persistence in the soil seedbank varied considerably across cultivars, years, and study locations. Scarification completely eliminated seedbank persistence 6 mo aft er burial but probably reduced viability and stand establishment in some cases. Among unscarified seed treatments, seedbank persistence ranged from < 1 to 7% 6 mo aft er burial. Burial of seed at 15 cm resulted in decreased seedling emergence and increased seedbank loss in comparison to the 3-cm burial depth. Among treatments, seedling emergence ranged from 36 to 84% at the 3-cm depth and 7 to 46% at the 15-cm depth. These results indicate that mechanical scarification is a potential strategy for managing hairy vetch seedbank persistence and warrants further investigation. Tillage may also be effective for decreasing hairy vetch seedbanks when coupled with other management tactics. C1 [Mirsky, Steven B.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Wallace, John M.; Curran, William S.; Crockett, Benjamin C.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. RP Mirsky, SB (reprint author), USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. EM steven.mirsky@ars.usda.gov NR 41 TC 1 Z9 1 U1 2 U2 9 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD NOV-DEC PY 2015 VL 107 IS 6 BP 2391 EP 2400 DI 10.2134/agronj14.0523 PG 10 WC Agronomy SC Agriculture GA DB2ML UT WOS:000368342900042 ER PT J AU Kornecki, TS AF Kornecki, T. S. TI RYE TERMINATION BY DIFFERENT ROLLERS/CRIMPERS DEVELOPED FOR NO-TILL SMALL-SCALE FARMS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Conservation agriculture; Cover crop; Roller/crimper; Termination rate; Walk-behind-tractors ID CONSERVATION TILLAGE; COVER CROPS; SOIL AB The expansion of locally grown fresh food by small vegetable farms must be associated with sustainable practices that include no-till systems with cover crops. In the Southern United States, the recommended time to plant cash crops into residue cover is typically three weeks after terminating the cover crop to minimize competition for resources between cover and cash crops. Rolling technology has been used to manage cover crops, and small-scale producers need rollers compatible with small tractors already used on farms. To address this need, afield experiment was conducted in 2012, 2013, and 2014 to determine the effectiveness of one commercial (curved) and two experimental rollers/crimpers (powered and two-stage) designed for walk-behind tractors. Rollers were operated at two speeds and rye was rolled one, two, and three times. Rye termination rates were evaluated one, two, and three weeks after rolling. In 2012, three weeks after rolling, all rollers generated similar termination rates (from 98.6% to 99.9%) In 2013, rye termination rates by all rollers were lower compared to 2012 due to higher rye biomass (17%), higher (14%) average soil volumetric moisture content (VMC) during the three week evaluation period (10% in 2012), and in 2013 rye was terminated at an earlier growth stage. In 2013, three weeks after rolling, higher rye termination rates were obtained by the powered roller (89.3%) and the two stage roller (86.6%), compared to the commercial roller (77.1%). In 2014, three weeks after rolling, rye termination rates were near 100% for all three roller types. In three growing seasons, at two weeks after rolling, rolling/crimping three times generated increased termination rates compared to rolling/crimping once or twice. Increasing operating speed generated slightly higher termination rates one week after rolling (2012 and 2014), whereas in 2013 these rates were higher one, two, and three weeks after rolling. In 2012, one week after rolling only, VMC for standing rye was significantly lower (5.4%) vs. 8.5% for rolled rye residue. In 2013, during three weeks of evaluation, an average VMC for standing rye was 9.1%, vs. higher VMC (14.1%) for all rolling treatments. In 2014 one and two weeks after rolling, average VMC was 16.9% for rolled residue vs. 13.6% for standing rye. VMC results from this study indicate that rolled residue better conserved soil moisture by superior soil coverage, due to a mulch effect. Overall, the experimental rollers/crimpers generated higher rye termination rates at one and two weeks after rolling, compared with the commercial roller. This can be applicable if weather delays cash crop planting thereby preventing the recommended three weeks between terminating cover crop and planting cash crop. These higher rates are most likely related to greater dynamic crimping forces of the experimental rollers, where crimping energy is released from springs, rather than relying on the limited weight of the roller as with the commercial roller. C1 [Kornecki, T. S.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL USA. RP Kornecki, TS (reprint author), 411 South Donahue Dr, Auburn, AL 36832 USA. EM ted.kornecki@ars.usda.gov NR 15 TC 0 Z9 0 U1 6 U2 10 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD NOV PY 2015 VL 31 IS 6 BP 849 EP 856 PG 8 WC Agricultural Engineering SC Agriculture GA DB5KS UT WOS:000368553600003 ER PT J AU Oliveira, JA Dominguez, JML Nearing, MA Oliveira, PTS AF Oliveira, J. A. Dominguez, J. M. L. Nearing, M. A. Oliveira, P. T. S. TI A GIS-BASED PROCEDURE FOR AUTOMATICALLY CALCULATING SOIL LOSS FROM THE UNIVERSAL SOIL LOSS EQUATION: GISUS-M SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE DEM; Geographic information system (GIS); NDVI; Soil erosion; USLE ID DIGITAL ELEVATION DATA; LS FACTOR; RUSLE; PREDICTION; MANAGEMENT; EXTRACTION; NETWORKS; EROSION; MODELS; USLE AB The integration of methods for calculating soil loss caused by water erosion using a geoprocessing system is important to enable investigations of soil erosion over large areas. Geographic information system (GIS)-based procedures have been used in soil erosion studies; however in most cases it is difficult to integrate the functionality in a single system to compute all soil loss factors. We developed a system able to combine all factors of the Universal Soil Loss Equation (USLE) with the computer functionality of a GIS. The GISus-M provides tools to compute the topographic (LS-factor) and cover and management (C-factor) from methods using remote sensing data. The other factors necessary to use the USLE, including soil erodibility, rainfall erosivity, and conservation practices, are also integrated in this tool. We describe in detail the GISus-M system and show its application in the Ribeirao do Salto sub-basin. From our new system, it is possible to work with different types of databases, making the GIS-procedure developed here a useful tool for researchers and decision makers to use spatial data and different methods to create future scenarios of soil erosion risk. C1 [Oliveira, J. A.] Univ Fed Reconcavo Bahia, Cruz Das Almas, BA, Brazil. [Dominguez, J. M. L.] Univ Fed Bahia, Res, Salvador, BA, Brazil. [Nearing, M. A.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [Oliveira, P. T. S.] Univ Sao Paulo, Dept Hydraul & Sanit Engn, Res, Sao Paulo, Brazil. RP Oliveira, JA (reprint author), Rui Barbosa 710, BR-44380000 Cruz Das Almas, BA, Brazil. EM joanito@ufba.br RI Oliveira, Paulo Tarso/A-4187-2011 OI Oliveira, Paulo Tarso/0000-0003-2806-0083 FU Fundacao de Amparo a Pesquisa da Bahia- FAPESB; CAPES Foundation, Ministry of Education of Brazil; CAPES; Sao Paulo Research Foundation [2015/05134-0] FX This work was initially supported by the Fundacao de Amparo a Pesquisa da Bahia- FAPESB and the latter part of this work was supported by CAPES Foundation, Ministry of Education of Brazil. In addition, the authors thank current support of the USDA-ARS-SWRC staff in Tucson. The fourth author was supported by CAPES and Sao Paulo Research Foundation (grant 2015/05134-0). We thank the anonymous reviewers for their very helpful comments and suggestions. NR 44 TC 1 Z9 1 U1 1 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD NOV PY 2015 VL 31 IS 6 BP 907 EP 917 PG 11 WC Agricultural Engineering SC Agriculture GA DB5KS UT WOS:000368553600009 ER PT J AU Chen, YHR Seabourn, BW Herald, TJ AF Chen, Yuanhong R. Seabourn, Bradfold W. Herald, Tom J. TI Modified Dough Preparation for Alveograph Analysis with Limited Flour Sample Size SO CEREAL CHEMISTRY LA English DT Article ID WHEAT-FLOUR; RHEOLOGICAL PROPERTIES; BAKING; QUALITY; BREAD AB Dough rheological characteristics obtained by alveograph testing, such as extensibility and resistance to extension, are important traits for determination of wheat and flour quality. A challenging issue that faces wheat breeding programs and some wheat research projects is the relatively large flour sample size of 250 g required for the standard alveograph method (AACCI Approved Method 54-30.02). A modified dough preparation procedure for a small flour sample size was developed for the alveograph test method. A dough was prepared by mixing 80 g of flour with 60% water absorption (2.5% salt solution) for 4 min in a 100 g pin mixer; it was then sheeted and cut into three patties of defined thickness. Data generated by the modified dough preparation method were significantly correlated with the results from the approved alveograph method. The correlation coefficients (r) for each of six alveograph dough characteristics of 40 different advanced breeding lines and wheat varieties were 0.92 for P (mm H2O), 0.73 for L (mm), 0.83 for W (10(-4) J), 0.90 for P/L, 0.90 for le (%), and 0.76 for G. The modified dough preparation was easier and more convenient than the approved method, and test time for the modified dough preparation was shorter by 20-25 min. This modified dough preparation procedure for the alveograph may be useful for wheat breeding programs as well as an alternative to the approved alveograph method for milling and baking industries and wheat quality research. C1 [Chen, Yuanhong R.; Seabourn, Bradfold W.; Herald, Tom J.] ARS, USDA, Hard Winter Wheat Qual Lab, Manhattan, KS 66502 USA. RP Chen, YHR (reprint author), ARS, USDA, Hard Winter Wheat Qual Lab, Manhattan, KS 66502 USA. EM richard.chen@ars.usda.gov NR 21 TC 0 Z9 0 U1 2 U2 6 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD NOV-DEC PY 2015 VL 92 IS 6 BP 565 EP 569 DI 10.1094/CCHEM-09-14-0197-N PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DB4UP UT WOS:000368509400005 ER EF