FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Vieira, P Wantoch, S Lilley, CJ Chitwood, DJ Atkinson, HJ Kamo, K AF Vieira, Paulo Wantoch, Sarah Lilley, Catherine J. Chitwood, David J. Atkinson, Howard J. Kamo, Kathryn TI Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. 'Nellie White' SO TRANSGENIC RESEARCH LA English DT Article DE Easter lilies; Pratylenchus; Cysteine proteinase inhibitor; Genetic engineering; Flower bulb crop ID AGROBACTERIUM-MEDIATED TRANSFORMATION; PRATYLENCHUS-PENETRANS; HAIRY ROOTS; ORYZACYSTATIN-I; PLANT CYSTATIN; PROTEINASE-INHIBITORS; ARABIDOPSIS-THALIANA; PARASITIC NEMATODES; GLOBODERA-PALLIDA; RICE PLANTS AB Lilium longiflorum cv. 'Nellie White' assumes a great economic importance as cut flowers, being one of the most valuable species (annual pot plants value above $20,000,000) in terms of wholesales in the US. The root lesion nematode Pratylenchus penetrans (RLN) constitutes one of the main pests for lily producers due to the significant root damage it causes. Our efforts have focused on the generation of soybean hairy roots (as a transient test model) and stable transgenic lilies overexpressing a modified rice cystatin (Oc-I Delta D86) transgene and challenged with root lesion nematodes. Lily transformation was achieved by gene gun co-bombardment using both a pBluescript-based vector containing the cystatin gene and pDM307 that contains a bar gene for phosphinothricin selection. Both soybean hairy roots and lilies overexpressing the OcI Delta D86 transgene exhibited enhanced resistance to RLN infection by means of nematode reduction up to 75 +/- A 5 % on the total number of nematodes. In addition, lily plants overexpressing OcI Delta D86 displayed an increase of plant mass and better growth performance in comparison to wild-type plants, thereby demonstrating an alternative strategy for increasing the yield and reducing nematode damage to this important floral crop. C1 [Vieira, Paulo; Wantoch, Sarah; Kamo, Kathryn] ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, USDA,BARC West, Beltsville, MD 20705 USA. [Lilley, Catherine J.; Atkinson, Howard J.] Univ Leeds, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England. [Chitwood, David J.] ARS, Nematol Lab, USDA, Beltsville, MD 20705 USA. RP Vieira, P (reprint author), ARS, Floral & Nursery Plants Res Unit, US Natl Arboretum, USDA,BARC West, 10300 Baltimore Ave,Bldg 010A Room 126, Beltsville, MD 20705 USA. EM paulo.vieira@ars.usda.gov OI Vieira, Paulo/0000-0001-5627-2628 FU Fred C. Gloeckner Foundation; California Department of Food and Agriculture FX This project was funded in part by the Fred C. Gloeckner Foundation and the California Department of Food and Agriculture 2012 Specialty Crop Block Grant awarded to Lee Riddle, Kathryn Kamo, and Rebecca Westerdahl. We thank Dr. Ben Matthews and Margaret McDonald (Soybean and Nematology Laboratory, USDA, Beltsville, MD) for the 'Williams 82' soybean seeds and for their helpful suggestions with the soybean hairy root system and Carol Masler (Nematology Laboratory, USDA, Beltsville, MD) for assisting with the nematode cultures. We thank Dr. Jonathan D. Eisenback for paper reviewing. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 55 TC 10 Z9 10 U1 3 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD JUN PY 2015 VL 24 IS 3 BP 421 EP 432 DI 10.1007/s11248-014-9848-2 PG 12 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CI4LJ UT WOS:000354720100004 PM 25398618 ER PT J AU Shepherd, LVT Alexander, CJ Hackett, CA McRae, D Sungurtas, JA Verrall, SR Morris, JA Hedley, PE Rockhold, D Belknap, W Davies, HV AF Shepherd, Louise Vida Traill Alexander, Colin James Hackett, Christine Anne McRae, Diane Sungurtas, Julia Anne Verrall, Susan Ramsay Morris, Jennifer Anne Hedley, Peter Edward Rockhold, David Belknap, William Davies, Howard Vivian TI Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers SO TRANSGENIC RESEARCH LA English DT Article DE Bruising; Genetic modification; Metabolomics; Potato; PPO; Transgenic ID EXPRESSION; TRANSFORMATION; TISSUE; GENES; CULTIVARS; PATHWAY; LIGHT; MS AB Tubers of potato (Solanum tuberosum L. cv. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography (GC)-MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using two PPO antisense lines (asPPO) and a wild-type (WT) control. Data were analysed using Principal Components Analysis and Analysis of Variance to assess differences between genotypes and temporal changes post-tuber wounding (by slicing). The levels of 15 metabolites (out of a total of 134 that were detected) differed between the WT and asPPO lines in mature tubers at harvest. A considerably higher number (63) of these metabolites changed significantly over a 48 h period following tuber wounding. For individual metabolites the magnitude of the differences between the WT and asPPO lines at harvest were small compared with the impacts of tuber wounding on metabolite levels. Some of the observed metabolite changes are explicable in terms of pathways known to be affected by wound responses. Whilst some statistically significant interactions (11 metabolites) were observed between line and time after wounding, very few profiles were consistent when comparing the WT with both asPPO lines, and the underlying metabolites appeared to be random in terms of the pathways they occupy. Overall, mechanical damage to tubers has a considerably greater impact on the metabolite profile than any potential unintended effects resulting from the down-regulation of PPO gene expression. C1 [Shepherd, Louise Vida Traill; McRae, Diane; Sungurtas, Julia Anne; Davies, Howard Vivian] James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. [Alexander, Colin James; Hackett, Christine Anne] Biomath & Stat Scotland, Dundee DD2 5DA, Scotland. [Verrall, Susan Ramsay] James Hutton Inst, Informat & Computat Sci Grp, Dundee DD2 5DA, Scotland. [Morris, Jennifer Anne; Hedley, Peter Edward] James Hutton Inst, Cell & Mol Sci Grp, Dundee DD2 5DA, Scotland. [Rockhold, David; Belknap, William] ARS, Crop Improvement & Utilizat Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Shepherd, LVT (reprint author), James Hutton Inst, Environm & Biochem Sci Grp, Dundee DD2 5DA, Scotland. EM louise.shepherd@hutton.ac.uk RI Alexander, Colin/C-5802-2009 OI Alexander, Colin/0000-0003-4995-1678 FU SAFEFOODS (EU) [Food-CT-2004-506446]; QualityLow InputFood (QLIF; EUFP6) [CT-2004-506358]; Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division FX This work was supported by SAFEFOODS (EU FP6 Contract No. Food-CT-2004-506446); QualityLow InputFood (QLIF; EUFP6 Contract No CT-2004-506358) and by the Scottish Government's Rural and Environment Science and Analytical Services (RESAS) Division. The authors would like to thank James W McNicol (Biomathematics and Statistics Scotland, Invergowrie, Dundee) for general statistical advice. The authors would also like to thank Sean Connor, Gary Dobson and Tom Shepherd for advice with data processing and metabolite identification. NR 38 TC 0 Z9 1 U1 3 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD JUN PY 2015 VL 24 IS 3 BP 447 EP 461 DI 10.1007/s11248-014-9850-8 PG 15 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CI4LJ UT WOS:000354720100006 PM 25417184 ER PT J AU Vail, AW Wang, P Uefuji, H Samac, DA Vance, CP Wackett, LP Sadowsky, MJ AF Vail, Andrew W. Wang, Ping Uefuji, Hirotaka Samac, Deborah A. Vance, Carroll P. Wackett, Lawrence P. Sadowsky, Michael J. TI Biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene SO TRANSGENIC RESEARCH LA English DT Article DE Atrazine; Transformation; Grasses; Transgenic plants; Biodegradation ID CONTAMINATED SOIL; PSEUDOMONAS SP; HUMAN CYP1A1; STRAIN ADP; AGROBACTERIUM-TUMEFACIENS; HERBICIDE ATRAZINE; PLANTS; PHYTOREMEDIATION; METABOLISM; SYSTEM AB The widespread use of atrazine and other s-triazine herbicides to control weeds in agricultural production fields has impacted surface and groundwater in the United States and elsewhere. We previously reported the cloning, sequencing, and expression of six genes involved in the atrazine biodegradation pathway of Pseudomonas sp. strain ADP, which is initiated by atzA, encoding atrazine chlorohydrolase. Here we explored the use of enhanced expression of a modified bacterial atrazine chlorohydrolase, p-AtzA, in transgenic grasses (tall fescue, perennial ryegrass, and switchgrass) and the legume alfalfa for the biodegradation of atrazine. Enhanced expression of p-AtzA was obtained by using combinations of the badnavirus promoter, the maize alcohol dehydrogenase first intron, and the maize ubiquitin promoter. For alfalfa, we used the first intron of the 5'-untranslated region tobacco alcohol dehydrogenase gene and the cassava vein mosaic virus promoter. Resistance of plants to atrazine in agar-based and hydroponic growth assays was correlated with in vivo levels of gene expression and atrazine degradation. The in planta expression of p-atzA enabled transgenic tall fescue to transform atrazine into hydroxyatrazine and other metabolites. Results of our studies highlight the potential use of transgenic plants for bioremediating atrazine in the environment. C1 [Vail, Andrew W.; Wang, Ping; Uefuji, Hirotaka; Wackett, Lawrence P.; Sadowsky, Michael J.] Univ Minnesota, BioTechnol Inst, St Paul, MN 55108 USA. [Samac, Deborah A.; Vance, Carroll P.] USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [Wackett, Lawrence P.] Univ Minnesota, Dept Biochem Mol Biol & Biophys, St Paul, MN 55108 USA. [Sadowsky, Michael J.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Sadowsky, MJ (reprint author), Univ Minnesota, BioTechnol Inst, 1479 Gortner Ave,140 Gortner Labs, St Paul, MN 55108 USA. EM Sadowsky@umn.edu RI Sadowsky, Michael/J-2507-2016 OI Sadowsky, Michael/0000-0001-8779-2781 FU Consortium for Plant Biotechnology Research; Syngenta Crop Protection FX This study was supported, in part, from Grants from The Consortium for Plant Biotechnology Research, and Syngenta Crop Protection. We would like to thank Melinda Dornbusch for watering plants in the greenhouse and William Koskinen and Brian Barber for help with HPLC and LC-MS analyses. The mention of a trade-mark, proprietary product or vendor does not constitute a guarantee or warranty of the product by the United States Department of Agriculture (USDA), and does not imply its approval to the exclusion of other products and vendors that might also be suitable. NR 51 TC 2 Z9 2 U1 3 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD JUN PY 2015 VL 24 IS 3 BP 475 EP 488 DI 10.1007/s11248-014-9851-7 PG 14 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CI4LJ UT WOS:000354720100008 PM 25432082 ER PT J AU Schneider, MJ Lehotay, SJ Lightfield, AR AF Schneider, Marilyn J. Lehotay, Steven J. Lightfield, Alan R. TI Validation of a streamlined multiclass, multiresidue method for determination of veterinary drug residues in bovine muscle by liquid chromatography-tandem mass spectrometry SO ANALYTICAL AND BIOANALYTICAL CHEMISTRY LA English DT Article DE Veterinary drug residue analysis; Filter-vial dispersive solid-phase extraction; Liquid chromatography-tandem mass spectrometry; Bovine muscle ID ELECTROSPRAY-IONIZATION; PERFORMANCE; EXTRACTION; KIDNEY; MS/MS; CLEANUP; FOODS AB Multiclass, multiresidue methods are becoming increasingly popular in regulatory monitoring programs due to their increased analytical scope and laboratory efficiency. In this work, we report the development and validation of a new high-throughput analytical method to monitor up to 131 veterinary drug residues, representing at least 13 different classes, in bovine muscle. This novel method streamlined sample preparation to < 15 min/sample/analyst, or a batch of 40-60 pre-homogenized samples in < 3 h/analyst, through the combination of dispersive solid-phase extraction with in-vial filtration (a new technique known as filter-vial d-SPE). The use of an enhanced sensitivity state-of-the-art tandem mass spectrometer led to < 10 ng/g limits of quantification for nearly all drug analytes with injection of 0.17 mg of equivalent sample. Positive and negative switching in electrospray ionization was applied to cover all analytes in an 11-min liquid chromatographic separation. In the 3-day validation study, 100 of the drugs met quantification criteria of 70-120 % recoveries and Horwitz Ratio a parts per thousand currency sign1.0, and the remaining analytes could still be screened at regulatory target levels. In the validation study involving > 11,400 analyte results for spiked samples, the rate of false negatives for identification purposes was < 5 %, and no false positives occurred at appreciable concentrations. C1 [Schneider, Marilyn J.; Lehotay, Steven J.; Lightfield, Alan R.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov NR 24 TC 8 Z9 8 U1 3 U2 28 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1618-2642 EI 1618-2650 J9 ANAL BIOANAL CHEM JI Anal. Bioanal. Chem. PD JUN PY 2015 VL 407 IS 15 BP 4423 EP 4435 DI 10.1007/s00216-014-8386-3 PG 13 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA CI2ZO UT WOS:000354617100020 PM 25542573 ER PT J AU Todd, RW Cole, NA Hagevoort, GR Casey, KD Auvermann, BW AF Todd, Richard W. Cole, N. Andy Hagevoort, G. Robert Casey, Kenneth D. Auvermann, Brent W. TI Ammonia losses and nitrogen partitioning at a southern High Plains open tot dairy SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Ammonia; Emissions; Dairy; Milk cows; Open lot; Inverse dispersion analysis ID BEEF-CATTLE FEEDYARD; OPEN-LOT DAIRIES; EMISSION FACTORS; CRUDE PROTEIN; WISCONSIN; METHANE; SUMMER; MANURE; IDAHO; FARMS AB Animal agriculture is a significant source of ammonia (NH3). Cattle excrete most ingested nitrogen (N); most urinary N is converted to NH3, volatilized and lost to the atmosphere. Open lot dairies on the southern High Plains are a growing industry and face environmental challenges as well as reporting requirements for NH3 emissions. We quantified NH3 emissions from the open lot and wastewater lagoons of a commercial New Mexico dairy during a nine-day summer campaign. The 3500-cow dairy consisted of open lot, manure-surfaced corrals (22.5 ha area). Lactating cows comprised 80% of the herd. A flush system using recycled wastewater intermittently removed manure from feeding alleys to three lagoons (1.8 ha area). Open path lasers measured atmospheric NH3 concentration, sonic anemometers characterized turbulence, and inverse dispersion analysis was used to quantify emissions. Ammonia fluxes (15-min) averaged 56 and 37 mu g m(-2) s(-1) at the open lot and lagoons, respectively. Ammonia emission rate averaged 1061 kg d(-1) at the open lot and 59 kg d-1 at the lagoons; 95% of NH3 was emitted from the open lot. The per capita emission rate of NH3 was 304 g cow(-1) d(-1) from the open lot (41% of N intake) and 17 g cow(-1) d(-1) from lagoons (2% of N intake). Daily N input at the dairy was 2139 kg d(-1), with 43, 36, 19 and 2% of the N partitioned to NH3 emission, manure/lagoons, milk, and cows, respectively. Published by Elsevier Ltd. C1 [Todd, Richard W.; Cole, N. Andy] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Hagevoort, G. Robert] New Mexico State Univ, Clovis, NM 88101 USA. [Casey, Kenneth D.; Auvermann, Brent W.] Texas A&M AgriLife Res, Amarillo, TX 79106 USA. RP Todd, RW (reprint author), USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. EM richard.todd@ars.usda.gov RI Casey, Kenneth/F-4409-2010; OI Casey, Kenneth/0000-0003-0819-8376; Hagevoort, G. Robert/0000-0002-1359-3912 FU USDA-CSREES Special Research [AG09-4288]; USDA-CSREES Special Research through the Southern Great Plains Dairy Consortium [2010-02674] FX This research was partially funded with USDA-CSREES Special Research Grant AG09-4288 and USDA-CSREES Special Research Grant 2010-02674 through the Southern Great Plains Dairy Consortium. NR 27 TC 4 Z9 4 U1 1 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD JUN PY 2015 VL 110 BP 75 EP 83 DI 10.1016/j.atmosenv.2015.02.069 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CI2QG UT WOS:000354591500009 ER PT J AU Hopken, MW Lum, TM Meyers, PM Piaggio, AJ AF Hopken, Matthew W. Lum, Tod M. Meyers, Paul M. Piaggio, Antoinette J. TI Molecular assessment of translocation and management of an endangered subspecies of white-tailed deer (Odocoileus virginianus) SO CONSERVATION GENETICS LA English DT Article DE Translocations; Genetic diversity; Subspecies; Introgression; Pacific Northwest ID MITOCHONDRIAL-DNA ANALYSIS; MULE DEER; INTRASPECIFIC VARIATION; GENETIC-RELATIONSHIPS; POPULATION-STRUCTURE; LOCAL ADAPTATION; WEST TEXAS; CONSERVATION; HYBRIDIZATION; RESTORATION AB Translocations are an effectual management strategy for the reestablishment and reconnection of endangered populations and species. However, knowledge about the evolution and ecology of the populations and species of interest are critical so that informed decisions can be made about source populations and reestablishment areas. We employed 614 base pairs of the mitochondrial control region and 15 microsatellite loci to investigate genetic variation, contemporary connectivity, and interspecific hybridization in the two remaining populations of the endangered Columbian white-tailed deer (Odocoileus virginianus leucurus) through comparisons with the closest subspecies, O. v. ochrourus. Our data revealed the dubious taxonomic status of O. v. leucurus, and that O. virginianus in the Pacific Northwest originated from a single historic gene pool. Further the results identified that populations are currently genetically isolated and depauperate, and uncovered historic introgression with O. hemionus columbianus. These results suggest that translocations are a viable approach for reestablishing populations throughout the historic range to increase genetic diversity in the fragmented populations. Despite the taxonomic ambiguity, our study revealed the presence of unique genetic variation within each population which supports ongoing conservation efforts. C1 [Hopken, Matthew W.; Piaggio, Antoinette J.] USDA APHIS WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Lum, Tod M.] Oregon Dept Fish & Wildlife, Roseburg, OR 97471 USA. [Meyers, Paul M.] US Fish & Wildlife Serv, Julia Butler Hansen Natl Wildlife Refuge, Cathlamet, WA 98612 USA. RP Hopken, MW (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM matt.w.hopken@aphis.usda.gov FU Oregon Department of Fish and Wildlife FX We would like to thank Shannon Wills and the Cowlitz Indian Tribe for samples, support, and consultation. At the Oregon Department of Fish and Wildlife we thank Hansel Hayden, Nick Leonetti, Martha Albritten, Leonard Erickson, and Richard Green for samples and funding. We also thank Kurt VerCauteren, Scott Hygnstrom of the United States Department of Agriculture National Wildlife Research Center, and Kenneth Warheit of the Washington Department of Fish and Wildlife for samples. Brett Coghlan, Julia Figueroa, Doreen Griffin, and Melissa Neubaum all contributed to the lab work. We would also like to exress our gratitude to two anonymous reviewers as they greatly improved this manuscript. NR 76 TC 0 Z9 0 U1 7 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD JUN PY 2015 VL 16 IS 3 BP 635 EP 647 DI 10.1007/s10592-014-0689-6 PG 13 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA CH9SC UT WOS:000354375500012 ER PT J AU Dolph, CL Eggert, SL Magner, J Ferrington, LC Vondracek, B AF Dolph, Christine L. Eggert, Susan L. Magner, Joe Ferrington, Leonard C., Jr. Vondracek, Bruce TI Reach-scale stream restoration in agricultural streams of southern Minnesota alters structural and functional responses of macroinvertebrates SO FRESHWATER SCIENCE LA English DT Article DE secondary production; biodiversity; richness; EPT; habitat heterogeneity; impaired waters; biomonitoring; dominant taxa ID SECONDARY PRODUCTION; LAND-USE; RIVER RESTORATION; MOUNTAIN STREAMS; ECOLOGY; COMMUNITIES; BIODIVERSITY; WATERSHEDS; MANAGEMENT; BOOTSTRAP AB Recent studies suggest that stream restoration at the reach scale may not increase stream biodiversity, raising concerns about the utility of this conservation practice. We examined whether reach-scale restoration in disturbed agricultural streams was associated with changes in macroinvertebrate community structure (total macroinvertebrate taxon richness, total macroinvertebrate density, Ephemeroptera, Plecoptera, Trichoptera [EPT] taxon richness, % abundance of EPT taxa) or secondary production (macroinvertebrate biomass over time). We collected macroinvertebrate samples over the course of 1 y from restored and unrestored reaches of 3 streams in southern Minnesota and used generalized least-square (GLS) models to assess whether measures of community structure were related to reach type, stream site, or sampling month. After accounting for effects of stream site and time, we found no significant difference in total taxon richness or % abundance of EPT taxa between restored and unrestored reaches. However, the number of EPT taxa and macroinvertebrate density were significantly higher in restored than in unrestored reaches. We compared secondary production estimates among study reaches based on 95th-percentile confidence intervals generated via bootstrapping. In each study stream, secondary production was significantly (2-3x) higher in the restored than in the unrestored reach. Higher productivity in the restored reaches was largely a result of the disproportionate success of a few dominant, tolerant taxa. Our findings suggest that reach-scale restoration may have ecological effects that are not detected by measures of total taxon richness alone. C1 [Dolph, Christine L.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Eggert, Susan L.] USDA ARS, No Res Stn, Grand Rapids, MN 55744 USA. [Magner, Joe] Univ Minnesota, Dept Bioprod & Biosyst Engn, Biosyst & Agr Engn 200, St Paul, MN 55108 USA. [Ferrington, Leonard C., Jr.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Vondracek, Bruce] Univ Minnesota, US Geol Survey, Minnesota Fish & Wildlife Cooperat Res Unit, St Paul, MN 55108 USA. RP Dolph, CL (reprint author), Univ Minnesota, Dept Ecol Evolut & Behav, 100 Ecol Bldg,1987 Upper Buford Circle, St Paul, MN 55108 USA. EM dolph008@umn.edu; seggert@fs.fed.us; magne027@umn.edu; ferri016@umn.edu; bvondrac@umn.edu FU Environmental Protection Agency Science To Achieve Results (STAR) Graduate Fellowship; University of Minnesota Butler and Jessen Water Resources Science Fellowship; MPCA 319 grant FX We thank Sally Entrekin, Denise Walther, and 2 anonymous referees for helpful suggestions that improved this manuscript. We thank Rochelle Roche for extensive field and laboratory assistance. This study was supported by an Environmental Protection Agency Science To Achieve Results (STAR) Graduate Fellowship, a University of Minnesota Butler and Jessen Water Resources Science Fellowship, and an MPCA 319 grant. Rhithron Associates, Inc. provided identification for a large portion of macroinvertebrate specimens collected in this study, including all Chironomidae. The use of trade, product, industry or firm names or products or software or models, whether commercially available or not, is for informative purposes only and does not constitute an endorsement by the US Government or the US Geological Survey. NR 49 TC 0 Z9 0 U1 8 U2 32 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2161-9549 EI 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD JUN PY 2015 VL 34 IS 2 BP 535 EP 546 DI 10.1086/680984 PG 12 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA CH9WR UT WOS:000354387700012 ER PT J AU Li, H Pan, XY Chen, LZ Zhang, A Wang, DX Wang, MQ AF Li, H. Pan, X. -Y. Chen, L. -Z. Zhang, A. Wang, D. -X. Wang, M. -Q. TI Expression profile and ligand-binding characterization of odorant-binding protein 2 from Batocera horsfieldi (Hope) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Batocera horsfieldi; expression pattern; fluorescence competition assay; odorant-binding proteins; volatiles ID MOLECULAR CHARACTERIZATION; CHEMOSENSORY PROTEINS; PHEROMONE-BINDING; GENES; INSECTS; IDENTIFICATION; RECEPTORS; PATTERNS; BEHAVIOR; TOOLS AB Odorant-binding proteins (OBPs) are important components in insect olfactory systems that transport semiochemicals through the aqueous sensillum lymph to surface of olfactory receptor neurons. In this study, we cloned the cDNA of odorant-binding protein 2 (BhorOBP2) in Batocera horsfieldi (Hope) and measured the level of the BhorOBP2 mRNA in a variety of tissues using quantitative reverse transcription and real-time polymerase chain reaction. We found that the BhorOBP2 mRNA was primarily expressed in the antennae, the level of BhorOBP2 mRNA was higher in males than in females, and correlated with age and mating status. The BhorOBP2 mRNA was also expressed in the labial palps at relatively high level, suggesting that it might have a role in gestation as well. To better understand the functions of the BhorOBP2 protein in odorant reception, recombinant BhorOBP2 was expressed in Escherichia coli, and the binding specificity of the protein for 18 volatile developmentally relevant chemicals and host-plant-related metabolites were assessed using N-phenyl-1-naphthylamine as a fluorescent probe. Competitive fluorescence binding assays showed that BhorOBP2 bound a broad range of host-plant-related odorants, including (E)-2-hexenal, 2-methyl-butanal, salicylaldehyde, (1S)-(-)-verbenone, (Z)-3-hexen-1-ol, (E)-2-hexen-1-ol, -pinene and ethyl acetate. Our results imply that BhorOBP2 might play an important role in the selectivity and specificity of odorant reception. C1 [Li, H.; Pan, X. -Y.; Chen, L. -Z.; Wang, M. -Q.] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Li, H.; Wang, D. -X.] Henan Univ Technol, Collaborat Innovat Ctr Henan Grain Crops, Henan Collaborat Innovat Ctr Grain Storage & Secu, Coll Food Sci & Technol, Zhengzhou 450000, Peoples R China. [Zhang, A.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, Beltsville, MD USA. RP Wang, MQ (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National Natural Science Foundation of China [30972374, 31170615]; Special Fund for Agro-Scientific Research in the Public Interest of China [201203036]; Fundamental Research Funds for the Central Universities program [2013PY046]; doctoral Foundation of Henan University of Technology [2014BS010] FX This study was funded by grants from the National Natural Science Foundation of China (grant nos. 30972374 and 31170615), the Special Fund for Agro-Scientific Research in the Public Interest of China (grant no. 201203036), the Fundamental Research Funds for the Central Universities program (grant no. 2013PY046) and the doctoral Foundation of Henan University of Technology (grant no. 2014BS010). NR 40 TC 1 Z9 1 U1 4 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2015 VL 139 IS 5 BP 361 EP 369 DI 10.1111/jen.12177 PG 9 WC Entomology SC Entomology GA CI2HI UT WOS:000354566300005 ER PT J AU Wells, JE Berry, ED Kalchayanand, N Rempel, LA Kim, M Oliver, WT AF Wells, J. E. Berry, E. D. Kalchayanand, N. Rempel, L. A. Kim, M. Oliver, W. T. TI Effect of lysozyme or antibiotics on faecal zoonotic pathogens in nursery pigs SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE alternative to antibiotics; antibiotics; lysozyme; pigs; swine ID IMPROVES GROWTH-PERFORMANCE; SMALL-INTESTINAL MORPHOLOGY; ESCHERICHIA-COLI; CAMPYLOBACTER-JEJUNI; MULTIPLEX PCR; UNITED-STATES; CATTLE FECES; GROWING-PIGS; MILK; PREVALENCE AB AimsThe objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. Methods and ResultsTwo replicates of approximately 650 pigs each were weaned and randomly assigned to one of 24 pens in either a nursery room that had been fully disinfected or a nursery room left unclean. Pigs were randomly assigned to control diet (Control), control diet+antibiotics (Antibiotic; chlortetracycline and tiamulin), or control diet+lysozyme (Lysozyme; 100mgkg(-1) diet). Rectal swab samples were collected on day 0 and 28 of treatment, and enriched and cultured for Campylobacter spp. and shiga-toxigenic Escherichia coli (STEC). Enrichments from rectal swab samples also were analysed for presence of enterohaemorrhagic E.coli (EHEC) virulence genes (hlyA, eae, stx1 and stx2). Room hygiene had little effect on day 28 results. Percentage of samples culture positive for Campylobacter spp. was lowest for lysozyme diets (P<001), but similar for control and antibiotic diets (432, 837, and 848 respectively). Diet had little effect on the EHEC virulence genes hlyA or eae (P>01), but there was a tendency for fewer samples positive for stx1/stx2 in antibiotic or lysozyme diet groups (P<007) compared to control diet (12, 21 and 58% respectively). Salmonella spp. and specific STEC types tested were rarely detected in the study. ConclusionsIn nursery swine, room hygiene had little effect on pathogen shedding. Dietary chlortetracycline and tiamulin did not reduce pathogen shedding but dietary lysozyme reduced faecal shedding of Campylobacter. Significance and Impact of the StudyLysozyme can effectively replace antibiotics in the diet of nursery swine and can be effective for pathogen control. C1 [Wells, J. E.; Berry, E. D.; Kalchayanand, N.; Rempel, L. A.; Kim, M.; Oliver, W. T.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Wells, JE (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM jim.wells@ars.usda.gov FU National Pork Board FX The thank Dee Kucera, Shannon Ostdiek, Shanda Watts, Bruce Jasch and Kathy Koch for technical assistance, Drs. Larry Kuehn and Kristin Hale for assistance with statistical analyses, and the USMARC Swine Operations for animal care and assistance with sample collection. The project was funded in part by the National Pork Board. NR 42 TC 2 Z9 3 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUN PY 2015 VL 118 IS 6 BP 1489 EP 1497 DI 10.1111/jam.12803 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CI2IT UT WOS:000354570600023 PM 25799945 ER PT J AU Dabney, SM Vieira, DAN Yoder, DC Langendoen, EJ Wells, RR Ursic, ME AF Dabney, S. M. Vieira, D. A. N. Yoder, D. C. Langendoen, E. J. Wells, R. R. Ursic, M. E. TI Spatially Distributed Sheet, Rill, and Ephemeral Gully Erosion SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Erosion; Deposition; Runoff; Revised universal soil loss equation; version 2 (RUSLE2); Sediment yield; Grassed waterway; Gully ID UPLAND CONCENTRATED FLOWS; SEDIMENT YIELD; SOIL-EROSION; RUNOFF; USLE; MORPHODYNAMICS; WEPP AB Ephemeral gully erosion seriously degrades agricultural soils, but few conservation planning tools adequately account for this form of erosion. To address this deficiency, this paper describes a spatially distributed adaptation of version 2 of the Revised Universal Soil Loss Equation and a new ephemeral gully erosion estimator. The modeled results were compared to runoff and sediment yield measured from 1975 to 1991 on a 6.3-ha instrumented watershed near Treynor, Iowa, managed with conventional tillage corn and containing a grassed waterway. Using a 3-m rectangular grid, this investigation determined surface drainage patterns and delineated concentrated flow channels where contributing areas exceeded 600m2. Computed gully evolution based on soil properties, runoff, and sediment transport contributed approximately one-fourth of the total erosion, with the rest contributed by sheet and rill erosion. More than half of the eroded sediment was deposited within the grassed waterway. Without local calibration, simulated runoff of 67mmyear-1 was 34% larger than the observed 50mmyear-1, and simulated sediment yield of 17.5Mgha-1year-1 was 20% larger than the measured 14.6Mgha-1year-1. C1 [Dabney, S. M.; Langendoen, E. J.; Wells, R. R.; Ursic, M. E.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Vieira, D. A. N.] USDA ARS, Natl Sedimentat Lab, State Univ, AR 72467 USA. [Yoder, D. C.] Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA. RP Dabney, SM (reprint author), USDA ARS, Natl Sedimentat Lab, Box 1157, Oxford, MS 38655 USA. EM seth.dabney@ars.usda.gov; dalmo.vieira@ars.usda.gov; dyoder@utk.edu; eddy.langendoen@ars.usda.gov; robert.wells@ars.usda.gov; mick.ursic@ars.usda.gov OI Langendoen, Eddy/0000-0002-2215-4989 NR 48 TC 2 Z9 2 U1 3 U2 33 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD JUN PY 2015 VL 20 IS 6 SI SI AR C4014009 DI 10.1061/(ASCE)HE.1943-5584.0001120 PG 12 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA CI2AU UT WOS:000354547700019 ER PT J AU Domingue, MJ Pulsifer, DP Lakhtakia, A Berkebile, J Steiner, KC Lelito, JP Hall, LP Baker, TC AF Domingue, Michael J. Pulsifer, Drew P. Lakhtakia, Akhlesh Berkebile, Jennifer Steiner, Kim C. Lelito, Jonathan P. Hall, Loyal P. Baker, Thomas C. TI Detecting emerald ash borers (Agrilus planipennis) using branch traps baited with 3D-printed beetle decoys SO JOURNAL OF PEST SCIENCE LA English DT Article DE 3D printing; Visual decoy; Forest pest; Invasive species; Visual attraction ID COLEOPTERA BUPRESTIDAE; MULTIFUNNEL TRAPS; MATING-BEHAVIOR; STICKY TRAPS; ATTRACTION; EFFICACY; COLOR; (Z)-3-HEXENOL; PLACEMENT; PHEROMONE AB Small visual-decoy-baited traps for the emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), have been researched as an alternative to current technologies, but have relied on real beetles rather than synthetic materials. We hypothesized that visual decoys created by three-dimensional (3D) printing can provide such a substitute. Branch traps displaying decoys consisting of real EAB females or 3D-printed decoys were compared to controls without decoys. Traps of the three varieties were placed on neighboring branches along with one (Z)-3-hexen-1-ol lure per tree and checked daily. Both real and 3D-printed decoys similarly increased EAB trap captures compared to controls. The numbers of both sexes were higher on the decoy-baited traps, but the increase in male captures was more pronounced. Males were also ensnared closer to the decoys than females. Daily trap-capture patterns showed sparse activity of EAB adults before June 18, 2013 followed by a peak in captures of both males and females until June 28, 2013. Beginning at approximately July 1, 2013, there was a second peak of EAB captures, which consisted almost entirely of males caught on the decoy-baited traps. The native ash borer Agrilus subcinctus was found earlier in the season and was also significantly attracted to both the real EABs and the 3D-printed decoys compared with control traps. Four purple prism traps were also deployed concurrently and captures tallied on three different days within the season. The results demonstrate efficacy of a small, inexpensive, and fully synthetic decoy-based branch trap system for EAB. C1 [Domingue, Michael J.; Hall, Loyal P.; Baker, Thomas C.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Pulsifer, Drew P.; Lakhtakia, Akhlesh] Penn State Univ, Dept Engn Sci & Mech, University Pk, PA 16802 USA. [Berkebile, Jennifer; Steiner, Kim C.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Lelito, Jonathan P.] USDA, Plant Protect & Quarantine Anim & Plant Hlth Insp, Brighton, MI USA. RP Domingue, MJ (reprint author), Penn State Univ, Dept Entomol, 119 Chem Ecol Lab, University Pk, PA 16802 USA. EM mjd29@psu.edu FU USDA-APHIS program [12-8130-1430-CA]; National Science Foundation [ECS-0335765] FX Funding was provided in part by the USDA-APHIS program supporting the Development of Detection Tools for Exotic Buprestid Beetles (12-8130-1430-CA). Jenna Ferraraccio and Dr. Justin George of Penn State University assisted in the collection of traps. Reflectance spectra were obtained by Josh Stapleton of the Pennsylvania State University Materials Research Institute Materials Characterization Laboratory, which is supported by the National Science Foundation Cooperative Agreement No. ECS-0335765. Dr. Leland Engel of Penn State University suggested the use of 3D printing for rapid production of inexpensive decoys. NR 39 TC 7 Z9 7 U1 9 U2 30 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2015 VL 88 IS 2 BP 267 EP 279 DI 10.1007/s10340-014-0598-y PG 13 WC Entomology SC Entomology GA CI3BD UT WOS:000354621500006 ER PT J AU Kraus, F Stahl, R Pitt, W AF Kraus, Fred Stahl, Randal Pitt, William TI Thermal fumigation provides a simple and effective solution for sanitizing cargo from invasive snakes SO JOURNAL OF PEST SCIENCE LA English DT Article DE Alien species; Boiga irregularis; Brown treesnake; Hot air ID BROWN TREE SNAKES; BOIGA-IRREGULARIS; GUAM; COLUBRIDAE; REPTILIA; ECOLOGY; BITES AB The Brown Treesnake (Boiga irregularis) is invasive in Guam and threatens to be dispersed by military and civilian transportation activities to other islands in the Pacific, where it could be expected to inflict similar damages. Prevention of inadvertent export of snakes in cargo and vehicles currently relies on trained canine detection teams, which are expensive to use and unable to detect all snakes. Hence, there has long been interest in developing effective and cheaper means of fumigating cargo to remove snakes. A companion study has shown that chemical fumigation is unlikely to be readily developed into a practical tool. Here, we demonstrate that these snakes are readily induced to quit test refugia by application of streams of heated air. Many parameters affect snake response times, but we find that application of relatively low temperatures (48-52 A degrees C) at moderate delivery rates (3.4 m(3)/min) is sufficient to induce exit of these snakes within 5 min. Development of a portable heat-delivery system based on these findings has great potential to ensure snakes do not unintentionally stow away to other locations in cargo, munitions, vehicles, or airplane wheelwells. Application of such technology can be done on Guam as well as at locations receiving cargo or vehicles from that source, providing an additional layer of security in ensuring these snakes do not colonize additional locations outside their native range. C1 [Kraus, Fred] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Stahl, Randal] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Pitt, William] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Hilo, HI 96721 USA. RP Kraus, F (reprint author), Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. EM fkraus@umich.edu FU Department of Interior's Office for Insular Affairs; Mississippi State University's Department of Wildlife, Fisheries Aquaculture FX This study was approved as NWRC Protocol QA-1837 and funded by the Department of Interior's Office for Insular Affairs. Support for initial work on this project was provided to FK by Mississippi State University's Department of Wildlife, Fisheries & Aquaculture. We thank C. Clark, J. Guerrero, T. Salas, and D. Vice for assistance on Guam, and the AAFB snake crew for providing us with test animals and for their kind tolerance of our invasion of their work space. NR 28 TC 1 Z9 1 U1 5 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2015 VL 88 IS 2 BP 331 EP 341 DI 10.1007/s10340-014-0627-x PG 11 WC Entomology SC Entomology GA CI3BD UT WOS:000354621500012 ER PT J AU Said, JI Song, MZ Wang, HT Lin, ZX Zhang, XL Fang, DD Zhang, JF AF Said, Joseph I. Song, Mingzhou Wang, Hantao Lin, Zhongxu Zhang, Xianlong Fang, David D. Zhang, Jinfa TI A comparative meta-analysis of QTL between intraspecific Gossypium hirsutum and interspecific G. hirsutum x G. barbadense populations SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Cotton; Gossypium hirsutum; Gossypium barbadense; Meta analysis; Quantitative trait loci ID QUANTITATIVE TRAIT LOCI; VERTICILLIUM WILT RESISTANCE; BACKCROSS-SELF APPROACH; COTTON FIBER QUALITY; 1ST FRUITING BRANCH; UPLAND COTTON; MOLECULAR DISSECTION; TETRAPLOID COTTON; RIL POPULATION; SSR MARKERS AB Based on 1075 and 1059 QTL from intraspecific Upland and interspecific Upland x Pima populations, respectively, the identification of QTL clusters and hotspots provides a useful resource for cotton breeding. Mapping of quantitative trait loci (QTL) is a pre-requisite of marker-assisted selection for crop yield and quality. Recent meta-analysis of QTL in tetraploid cotton (Gossypium spp.) has identified regions of the genome with high concentrations of QTL for various traits called clusters and specific trait QTL called hotspots or meta-QTL (mQTL). However, the meta-analysis included all population types of Gossypium mixing both intraspecific G. hirsutum and interspecific G. hirsutum x G. barbadense populations. This study used 1,075 QTL from 58 publications on intraspecific G. hirsutum and 1,059 QTL from 30 publications on G. hirsutum x G. barbadense populations to perform a comprehensive comparative analysis of QTL clusters and hotspots between the two populations for yield, fiber and seed quality, and biotic and abiotic stress tolerance. QTL hotspots were further analyzed for mQTL within the hotspots using Biomercator V3 software. The ratio of QTL between the two population types was proportional yet differences in hotspot type and placement were observed between the two population types. However, on some chromosomes QTL clusters and hotspots were similar between the two populations. This shows that there are some universal QTL regions in the cultivated tetraploid cotton which remain consistent and some regions which differ between population types. This study for the first time elucidates the similarities and differences in QTL clusters and hotspots between intraspecific and interspecific populations, providing an important resource to cotton breeding programs in marker-assisted selection . C1 [Said, Joseph I.; Zhang, Jinfa] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Song, Mingzhou] New Mexico State Univ, Dept Comp Sci, Las Cruces, NM 88003 USA. [Wang, Hantao; Lin, Zhongxu; Zhang, Xianlong] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Wang, Hantao; Lin, Zhongxu; Zhang, Xianlong] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China. [Fang, David D.] USDA ARS SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM joesaid@nmsu.edu; joemsong@nmsu.edu; w.wanghantao@163.com; linzhongxu@mail.hzau.edu.cn; xlzhang@mail.hzau.edu.cn; david.fang@ars.usda.gov; jinzhang@nmsu.edu NR 98 TC 24 Z9 24 U1 6 U2 39 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD JUN PY 2015 VL 290 IS 3 BP 1003 EP 1025 DI 10.1007/s00438-014-0963-9 PG 23 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA CI3CP UT WOS:000354625500014 PM 25501533 ER PT J AU Chopra, R Burow, G Farmer, A Mudge, J Simpson, CE Wilkins, TA Baring, MR Puppala, N Chamberlin, KD Burow, MD AF Chopra, Ratan Burow, Gloria Farmer, Andrew Mudge, Joann Simpson, Charles E. Wilkins, Thea A. Baring, Michael R. Puppala, Naveen Chamberlin, Kelly D. Burow, Mark D. TI Next-generation transcriptome sequencing, SNP discovery and validation in four market classes of peanut, Arachis hypogaea L. SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Peanut; Groundnut; Arachis; Transcriptome; SNP; KASP ID GENETIC-LINKAGE MAP; DELTA(12)-FATTY ACID DESATURASE; MINI CORE COLLECTION; HIGH OLEATE TRAIT; CULTIVATED PEANUT; RNA-SEQ; GERMPLASM COLLECTION; MESSENGER-RNA; FAD2 GENE; REGISTRATION AB Single-nucleotide polymorphisms, which can be identified in the thousands or millions from comparisons of transcriptome or genome sequences, are ideally suited for making high-resolution genetic maps, investigating population evolutionary history, and discovering marker-trait linkages. Despite significant results from their use in human genetics, progress in identification and use in plants, and particularly polyploid plants, has lagged. As part of a long-term project to identify and use SNPs suitable for these purposes in cultivated peanut, which is tetraploid, we generated transcriptome sequences of four peanut cultivars, namely OLin, New Mexico Valencia C, Tamrun OL07 and Jupiter, which represent the four major market classes of peanut grown in the world, and which are important economically to the US southwest peanut growing region. CopyDNA libraries of each genotype were used to generate 2 x 54 paired-end reads using an Illumina GAIIx sequencer. Raw reads were mapped to a custom reference consisting of Tifrunner 454 sequences plus peanut ESTs in GenBank, compromising 43,108 contigs; 263,840 SNP and indel variants were identified among four genotypes compared to the reference. A subset of 6 variants was assayed across 24 genotypes representing four market types using KASP chemistry to assess the criteria for SNP selection. Results demonstrated that transcriptome sequencing can identify SNPs usable as selectable DNA-based markers in complex polyploid species such as peanut. Criteria for effective use of SNPs as markers are discussed in this context. C1 [Chopra, Ratan; Wilkins, Thea A.; Burow, Mark D.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Burow, Gloria] ARS, USDA, CSRL, Lubbock, TX 79415 USA. [Farmer, Andrew; Mudge, Joann] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Simpson, Charles E.] Texas A&M AgriLife Res, Stephenville, TX 76401 USA. [Baring, Michael R.] Texas A&M AgriLife Res, Heep Ctr, College Stn, TX 77843 USA. [Puppala, Naveen] New Mexico State Univ, Agr Sci Ctr, Clovis, NM 88001 USA. [Chamberlin, Kelly D.] ARS, USDA, Stillwater, OK 74075 USA. [Burow, Mark D.] Texas A&M AgriLife Res, Lubbock, TX 79403 USA. RP Burow, MD (reprint author), Texas A&M AgriLife Res, 1102 East FM 1294, Lubbock, TX 79403 USA. EM mburow@tamu.edu OI CHOPRA, RATAN/0000-0003-2088-3341 FU Texas Peanut Producers Board; National Peanut Board [332/TX-99/1139, 332/TX-99/1213]; Peanut Foundation [04-810-08]; Ogallala Aquifer Initiative award [IPM12.06]; United States Department of Agriculture/National Institute of Food and Agriculture Hatch Act award [TEX08835] FX The authors wish to thank Jennifer Chagoya at Texas A&M AgriLife Research, and Halee Hughes and Nancy Layland at the USDA-ARS, Lubbock for technical support. This work was funded by grants from the Texas Peanut Producers Board award CY2008-Burow-TTU-Development to MDB and CES, and 2009-TTU-Burow-Genotyping to MDB, National Peanut Board grant #332/TX-99/1139 to MDB, and #332/TX-99/1213 to MDB and CES, Peanut Foundation grant 04-810-08 to MDB, Ogallala Aquifer Initiative award IPM12.06 to MDB, and United States Department of Agriculture/National Institute of Food and Agriculture Hatch Act award TEX08835 to MDB. NR 66 TC 6 Z9 6 U1 4 U2 50 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD JUN PY 2015 VL 290 IS 3 BP 1169 EP 1180 DI 10.1007/s00438-014-0976-4 PG 12 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA CI3CP UT WOS:000354625500026 PM 25663138 ER PT J AU Nachman, RJ AF Nachman, Ronald J. TI Introduction: Invertebrate Neuropeptides XV SO PEPTIDES LA English DT Editorial Material C1 USDA, Insect Neuropeptide Lab, Insect Control & Cotton Dis Res, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Nachman, RJ (reprint author), USDA, Insect Neuropeptide Lab, Insect Control & Cotton Dis Res, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. EM nachman@tamu.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 EI 1873-5169 J9 PEPTIDES JI Peptides PD JUN PY 2015 VL 68 BP 1 EP 2 DI 10.1016/j.peptides.2015.04.005 PG 2 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA CI2JF UT WOS:000354572200001 PM 25981410 ER PT J AU Jiang, HB Wei, ZJ Nachman, RJ Kaczmarek, K Zabrocki, J Park, YS AF Jiang, Hongbo Wei, Zhaojun Nachman, Ronald J. Kaczmarek, Krzysztof Zabrocki, Janusz Park, Yoonseong TI Functional characterization of five different PRXamide receptors of the red flour beetle Tribolium castaneum with peptidomimetics and identification of agonists and antagonists SO PEPTIDES LA English DT Article DE GPCR; Pyrokinin; CAPA; PBAN; ETH; pharmacology ID BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE; PROTEIN-COUPLED RECEPTOR; SEX-PHEROMONE PRODUCTION; DIAPAUSE HORMONE; MOLECULAR-CLONING; HELICOVERPA-ZEA; PUPAL DIAPAUSE; CORN-EARWORM; PK/PBAN ANALOG; AEDES-AEGYPTI AB The neuropeptidergic system in insects is an excellent target for pest control strategies. One promising biorational approach is the use of peptidomimetics modified from endogenous ligands to enhance biostability and bioavailability. In this study, we functionally characterized five different G protein-coupled receptors in a phylogenetic cluster, containing receptors for PRXamide in the red flour beetle Tribolium castaneum, by evaluating a series of 70 different peptides and peptidomimetics. Three pyrokinin receptors (TcPKr-A, -B, and -C), cardioacceleratory peptide receptor (TcCAPAr) and ecdysis triggering hormone receptor (TcETHr) were included in the study. Strong agonistic or antagonistic peptidomimetics were identified, and included beta-proline (beta P-3) modification of the core amino acid residue proline and also a cyclo-peptide. It is common for a ligand to act on multiple receptors. In a number of cases, a ligand acting as an agonist on one receptor was an efficient antagonist on another receptor, suggesting complex outcomes of a peptidomimetic in a biological system. Interestingly, TcPK-A was highly promiscuous with a high number of agonists, while TcPK-C and TcCAPAr had a lower number of agonists, but a higher number of compounds acting as an antagonist. This observation suggests that a target GPCR with more promiscuity will provide better success for peptidomimetic approaches. This study is the first description of peptidomimetics on a CAPA receptor and resulted in the identification of peptidomimetic analogs that demonstrate antagonism of CAPA ligands. The PRXamide receptor assays with peptidomimetics provide useful insights into the biochemical properties of receptors. (C) 2014 Elsevier Inc. All rights reserved. C1 [Jiang, Hongbo] Southwest Univ, Coll Plant Protect, Lab Entomol & Pest Control Engn, Chongqing 400715, Peoples R China. [Jiang, Hongbo; Wei, Zhaojun; Park, Yoonseong] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Nachman, Ronald J.; Kaczmarek, Krzysztof; Zabrocki, Janusz] USDA, Southern Plains Agr Res Ctr, Insect Control & Cotton Dis Res Unit, College Stn, TX 77845 USA. [Kaczmarek, Krzysztof; Zabrocki, Janusz] Lodz Univ Technol, Inst Organ Chem, PL-90924 Lodz, Poland. RP Park, YS (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM ypark@ksu.edu OI Wei, Zhao-Jun/0000-0003-1729-209X FU Kansas Agricultural Experiment Station [15-122-J]; National Institutes of Health [R01AI090062]; National Nature Science Foundation of China [31201508]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20120182120019]; USDA/DOD DWFP Initiative [6202-22000-029-00D]; United States-Israel Binational Agricultural Research and Development Fund (BARD) [IS-4205-09C] FX This paper is contribution no. 15-122-J from the Kansas Agricultural Experiment Station. HJ and YP were supported in part by National Institutes of Health; Grant Number: R01AI090062, National Nature Science Foundation of China (31201508) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20120182120019). RJN, KK and JZ were supported in part by the USDA/DOD DWFP Initiative (#6202-22000-029-00D) and United States-Israel Binational Agricultural Research and Development Fund (BARD) (IS-4205-09C). NR 44 TC 5 Z9 5 U1 3 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 EI 1873-5169 J9 PEPTIDES JI Peptides PD JUN PY 2015 VL 68 BP 246 EP 252 DI 10.1016/j.peptides.2014.11.004 PG 7 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA CI2JF UT WOS:000354572200035 PM 25447413 ER PT J AU Gealy, DR AF Gealy, D. R. TI Deep phosphorus fertiliser placement and reduced irrigation methods for rice (Oryza sativa L.) combine to knock-out competition from its nemesis, barnyard grass (Echinochloa crus-galli (L.) P. Beauv) SO PLANT AND SOIL LA English DT Editorial Material DE C-3; C-4; Nutrient-poor soil; Phosphorus fertilizer placement; Barnyardgrass; Echinochloa crus-galli; Rice; Oryza sativa; Weed competition; Root and plant growth; Alternating wetting and drying; AWD; Topsoil drying; TSD; Irrigation ID DIRECT-SEEDED RICE; ISOTOPE DISCRIMINATION ANALYSIS; WEED-SUPPRESSIVE RICE; RELATIVE IMPORTANCE; ROOT SYSTEMS; C-4 PHOTOSYNTHESIS; SHOOT COMPETITION; UPLAND RICE; WATER; GROWTH C1 ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Gealy, DR (reprint author), ARS, USDA, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 East, Stuttgart, AR 72160 USA. EM david.gealy@ars.usda.gov NR 71 TC 0 Z9 2 U1 7 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD JUN PY 2015 VL 391 IS 1-2 BP 427 EP 431 DI 10.1007/s11104-015-2478-5 PG 5 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA CH7DZ UT WOS:000354197100030 ER PT J AU Maccaferri, M Ricci, A Salvi, S Milner, SG Noli, E Martelli, PL Casadio, R Akhunov, E Scalabrin, S Vendramin, V Ammar, K Blanco, A Desiderio, F Distelfeld, A Dubcovsky, J Fahima, T Faris, J Korol, A Massi, A Mastrangelo, AM Morgante, M Pozniak, C N'Diaye, A Xu, S Tuberosa, R AF Maccaferri, Marco Ricci, Andrea Salvi, Silvio Milner, Sara Giulia Noli, Enrico Martelli, Pier Luigi Casadio, Rita Akhunov, Eduard Scalabrin, Simone Vendramin, Vera Ammar, Karim Blanco, Antonio Desiderio, Francesca Distelfeld, Assaf Dubcovsky, Jorge Fahima, Tzion Faris, Justin Korol, Abraham Massi, Andrea Mastrangelo, Anna Maria Morgante, Michele Pozniak, Curtis N'Diaye, Amidou Xu, Steven Tuberosa, Roberto TI A high-density, SNP-based consensus map of tetraploid wheat as a bridge to integrate durum and bread wheat genomics and breeding SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE durum wheat; single nucleotide polymorphism; consensus map; genomics-assisted breeding; homeologous loci; chromosome translocation events; anchor markers ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; NUCLEOTIDE POLYMORPHISM MARKERS; GENETIC-LINKAGE MAP; HEXAPLOID WHEAT; POLYPLOID WHEAT; GRAIN-YIELD; DART MARKERS; DOMESTICATION; MICROSATELLITE AB Consensus linkage maps are important tools in crop genomics. We have assembled a high-density tetraploid wheat consensus map by integrating 13 data sets from independent biparental populations involving durum wheat cultivars (Triticum turgidum ssp.durum), cultivated emmer (T.turgidum ssp.dicoccum) and their ancestor (wild emmer, T.turgidum ssp.dicoccoides). The consensus map harboured 30144 markers (including 26626 SNPs and 791 SSRs) half of which were present in at least two component maps. The final map spanned 2631cM of all 14 durum wheat chromosomes and, differently from the individual component maps, all markers fell within the 14 linkage groups. Marker density per genetic distance unit peaked at centromeric regions, likely due to a combination of low recombination rate in the centromeric regions and even gene distribution along the chromosomes. Comparisons with bread wheat indicated fewer regions with recombination suppression, making this consensus map valuable for mapping in the A and B genomes of both durum and bread wheat. Sequence similarity analysis allowed us to relate mapped gene-derived SNPs to chromosome-specific transcripts. Dense patterns of homeologous relationships have been established between the A- and B-genome maps and between nonsyntenic homeologous chromosome regions as well, the latter tracing to ancient translocation events. The gene-based homeologous relationships are valuable to infer the map location of homeologs of target loci/QTLs. Because most SNP and SSR markers were previously mapped in bread wheat, this consensus map will facilitate a more effective integration and exploitation of genes and QTL for wheat breeding purposes. C1 [Maccaferri, Marco; Ricci, Andrea; Salvi, Silvio; Milner, Sara Giulia; Noli, Enrico; Tuberosa, Roberto] Univ Bologna, Dept Agr Sci DipSA, Bologna, Italy. [Martelli, Pier Luigi; Casadio, Rita] Univ Bologna, Biocomp Grp, Bologna, Italy. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Scalabrin, Simone; Vendramin, Vera; Morgante, Michele] Ist Genom Applicata, Udine, Italy. [Scalabrin, Simone; Vendramin, Vera; Morgante, Michele] Univ Udine, Dipartimento Sci Agr & Ambientali, I-33100 Udine, Italy. [Ammar, Karim] CIMMYT Carretera Mexico, Texcoco, Mexico. [Blanco, Antonio] Univ Bari, Dipartimento Biol Chim Agroforestale & Ambientale, Bari, Italy. [Desiderio, Francesca] Consiglio Ric & Sperimentaz Agr, Genom Res Ctr, Fiorenzuola Darda, Italy. [Distelfeld, Assaf] Tel Aviv Univ, Fac Life Sci, Dept Mol Biol & Ecol Plants, IL-69978 Tel Aviv, Israel. [Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD USA. [Fahima, Tzion; Korol, Abraham] Univ Haifa, Inst Evolut, Dept Evolutionary & Environm Biol, Fac Sci & Sci Educ, IL-31999 Haifa, Israel. [Faris, Justin; Xu, Steven] USDA ARS, Cereal Crops Res Unit, Fargo, ND USA. [Massi, Andrea] Soc Produttori Sementi Bologna PSB, Argelato, Italy. [Mastrangelo, Anna Maria] Consiglio Ric & Sperimentaz Agr, Cereal Res Ctr, Foggia, Italy. [Pozniak, Curtis; N'Diaye, Amidou] Univ Saskatchewan, Ctr Crop Dev, Saskatoon, SK S7N 0W0, Canada. [Pozniak, Curtis; N'Diaye, Amidou] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK, Canada. RP Maccaferri, M (reprint author), Univ Bologna, Dept Agr Sci DipSA, Bologna, Italy. EM marco.maccaferri@unibo.it RI Casadio, Rita/K-4814-2015; Tuberosa, Roberto/A-2781-2016; Dubcovsky, Jorge/A-4969-2008; OI Casadio, Rita/0000-0002-7462-7039; Tuberosa, Roberto/0000-0001-9143-9569; Dubcovsky, Jorge/0000-0002-7571-4345; BLANCO, ANTONIO/0000-0002-2222-1133; Mastrangelo, Anna Maria/0000-0002-3618-0301 FU AGER Agroalimentare e Ricerca-Project 'From seed to pasta-Multidisciplinary approaches for a more sustainable and high-quality durum wheat production; Agriculture and Agri-Food Canada; Genome Canada; Genome Prairie; Saskatchewan Ministry of Agriculture; Western Grains Research Foundation; National Research Initiative Competitive Grants [2011-68002-30029]; special grant ISCOCEM FX We wish to thank Maria C. Sanguineti for her help and suggestions in the statistical analysis of the data sets. Research supported by the AGER Agroalimentare e Ricerca-Project 'From seed to pasta-Multidisciplinary approaches for a more sustainable and high-quality durum wheat production. CJP acknowledges the financial support of Agriculture and Agri-Food Canada, Genome Canada, Genome Prairie, The Saskatchewan Ministry of Agriculture and the Western Grains Research Foundation. EA and JD acknowledge the financial support of the National Research Initiative Competitive Grants 2011-68002-30029 (Triticeae-CAP). AMM acknowledges the financial support of the special grant ISCOCEM. NR 82 TC 30 Z9 30 U1 7 U2 66 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD JUN PY 2015 VL 13 IS 5 BP 648 EP 663 DI 10.1111/pbi.12288 PG 16 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CI2GD UT WOS:000354562700005 PM 25424506 ER PT J AU Stevanato, P Trebbi, D Panella, L Richardson, K Broccanello, C Pakish, L Fenwick, AL Saccomani, M AF Stevanato, Piergiorgio Trebbi, Daniele Panella, Lee Richardson, Kelley Broccanello, Chiara Pakish, Linda Fenwick, Ann L. Saccomani, Massimo TI Identification and Validation of a SNP Marker Linked to the Gene HsBvm-1 for Nematode Resistance in Sugar Beet SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE HsBvm-1; Beta vulgaris ssp maritima; Biotic stresses; Beet-cyst nematode; WB242 genetic tolerance; SNP ID BULKED SEGREGANT ANALYSIS; RHIZOMANIA RESISTANCE; DISCOVERY; GENOME AB The beet-cyst nematode (Heterodera schachtii Schmidt) is one of the major pests of sugar beet. The identification of molecular markers associated with nematode tolerance would be helpful for developing tolerant varieties. The aim of this study was to identify single nucleotide polymorphism (SNP) markers linked to nematode tolerance from the Beta vulgaris ssp. maritima source WB242. A WB242-derived F-2 population was phenotyped for host-plant nematode reaction revealing a 3:1 segregation ratio of the tolerant and susceptible phenotypes and suggesting the action of a gene designated as HsBvm-1. Bulked segregant analysis (BSA) was used. The most tolerant and susceptible individuals were pooled and subjected to restriction site associated DNA sequencing (RAD-Seq) analysis, which identified 7,241 SNPs. A subset of 384 candidate SNPs segregating between bulks were genotyped on the 20 most-tolerant and most-susceptible individuals, identifying a single marker (SNP192) showing complete association with nematode tolerance. Segregation of SNP192 confirmed the inheritance of tolerance by a single gene. This association was further validated on a set of 26 commercial tolerant and susceptible varieties, showing the presence of the SNP192 WB242-type allele only in the tolerant varieties. We identified and mapped on chromosome 5 the first nematode tolerance gene (HsBvm-1) from Beta vulgaris ssp. maritima and released information on SNP192, a linked marker valuable for high-throughput, marker-assisted breeding of nematode tolerance in sugar beet. C1 [Stevanato, Piergiorgio; Trebbi, Daniele; Broccanello, Chiara; Saccomani, Massimo] Univ Padua, DAFNAE, Dipartimento Agron Anim Alimenti Risorse Nat & Am, I-35020 Legnaro, PD, Italy. [Panella, Lee] USDA ARS, NPA, Sugarbeet Res Unit, Crops Res Lab, Ft Collins, CO 80526 USA. [Richardson, Kelley; Pakish, Linda] USDA ARS, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. [Fenwick, Ann L.] Beet Sugar Dev Fdn, Crops Res Lab, Ft Collins, CO 80526 USA. RP Stevanato, P (reprint author), Univ Padua, DAFNAE, Dipartimento Agron Anim Alimenti Risorse Nat & Am, Viale Univ 16, I-35020 Legnaro, PD, Italy. EM stevanato@unipd.it NR 23 TC 2 Z9 3 U1 5 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2015 VL 33 IS 3 BP 474 EP 479 DI 10.1007/s11105-014-0763-8 PG 6 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CI1HV UT WOS:000354495500012 ER PT J AU Ali, A Bang, SW Chung, SM Staub, JE AF Ali, Asjad Bang, Sun Woong Chung, Sang-Min Staub, Jack E. TI Plant Transformation via Pollen Tube-Mediated Gene Transfer SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Molecular breeding; Ovary drip; Plant improvement; Pollen tube pathway ID ZEA-MAYS L.; AGROBACTERIUM-TUMEFACIENS; TRITICUM-AESTIVUM; SELECTABLE MARKER; EXOGENOUS DNA; FLORAL DIP; PATHWAY; MAIZE; CASSETTE; COTTON AB Genetic transformation using foreign genes and the subsequent development of transgenic plants has been employed to develop enhanced elite germplasm. Although some skepticism exits regarding pollen tube-mediated gene transfer (PTT), reports demonstrating improved transformation efficiency with PTT systems are increasing and encouraging and the adoption of increasingly refined pollen-mediated methodologies may lead to species-dependent improvements in breeding. Here, we highlight PTT technology as an alternative to genetic transformation. C1 [Ali, Asjad; Bang, Sun Woong; Chung, Sang-Min] Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. [Staub, Jack E.] ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. RP Chung, SM (reprint author), Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. EM smchung@dongguk.edu FU Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry, and Fisheries, Republic of Korea [114078031HD020] FX This work was supported by grants from the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry, and Fisheries [grant number 114078031HD020], Republic of Korea. NR 49 TC 0 Z9 0 U1 4 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2015 VL 33 IS 3 BP 742 EP 747 DI 10.1007/s11105-014-0839-5 PG 6 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CI1HV UT WOS:000354495500033 ER PT J AU Yu, XQ Pijut, PM Byrne, S Asp, T Bai, GH Jiang, YW AF Yu, Xiaoqing Pijut, Paula M. Byrne, Stephen Asp, Torben Bai, Guihua Jiang, Yiwei TI Candidate gene association mapping for winter survival and spring regrowth in perennial ryegrass SO PLANT SCIENCE LA English DT Article DE Association mapping; Candidate gene; Lolium perenne; Winter survival; Regrowth ID GENOME-WIDE ASSOCIATION; COLD-ACCLIMATION; FREEZING TOLERANCE; LOLIUM-PERENNE; NATURAL VARIATION; ARABIDOPSIS-THALIANA; POPULATION-STRUCTURE; FROST TOLERANCE; WATER-DEFICIT; TRANSCRIPTOME ANALYSIS AB Perennial iyegrass (Lolium perenne L) is a widely cultivated cool-season grass species because of its high quality for forage and turf. Susceptibility to freezing damage limits its further use in temperate zones. The objective of this study was to identify candidate genes significantly associated with winter survival and spring regrowth in a global collection of 192 perennial ryegrass accessions. Significant differences in winter survival (WS), percentage of canopy green cover (CGC), chlorophyll index (Chl), and normalized difference vegetation index (NDVI) were found among accessions. After controlling population structure, LpLEA3 encoding a late embryogenesis abundant group 3 protein and LpCAT encoding a catalase were associated with CGC and Chl, while LpMnSOD encoding a magnesium superoxide dismutase and LpChl Cu-ZnSOD encoding a chlorophyll copper zinc superoxide dismutase were associated with NDVI or Chl. Significant association was also discovered between C-repeat binding factor LpCBF1b and WS. Three sequence variations identified in LpCAT, LpMnSOD, and LpChl Cu-ZnSOD were synonymous substitutions, whereas one pair of adjacent single nucleotide polymorphisms (SNPs) in LpLEA3 and one SNP in LpCBF1b resulted in amino acid change. The results demonstrated that allelic variation in LpLEA3 and LpCBF1b was closely related to winter survival and spring regrowth in perennial ryegrass. (C) 2015 Elsevier Ireland Ltd. All rights reserved. C1 [Yu, Xiaoqing] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Pijut, Paula M.] USDAForest Serv, No Res Stn, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Byrne, Stephen; Asp, Torben] Aarhus Univ, Res Ctr Flakkebjerg, Fac Sci & Technol, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark. [Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Jiang, Yiwei] Purdue Univ, Dept Agron, W Lafayette, IN 47927 USA. RP Jiang, YW (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47927 USA. EM yjiang@purdue.edu RI Pijut, Paula/N-6789-2015; OI Byrne, Stephen/0000-0002-1179-2272 FU O.J. Noer Research Foundation; Midwest Regional Turfgrass Foundation; Purdue University Research Foundation FX The authors would like to thank Drs. Zijian Sun, Shuwei Liu, Na Luo, Ying Wang, and Jinchi Tang for assisting in gene sequencing, molecular marker analysis and phenotypic trait evaluation. This work was supported by the O.J. Noer Research Foundation, The Midwest Regional Turfgrass Foundation, and Purdue University Research Foundation. NR 69 TC 5 Z9 5 U1 4 U2 38 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD JUN PY 2015 VL 235 BP 37 EP 45 DI 10.1016/j.plantsci.2015.03.003 PG 9 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CI2QI UT WOS:000354591700004 PM 25900564 ER PT J AU Konter, O Esper, J Liebhold, A Kyncl, T Schneider, L Duthorn, E Buntgen, U AF Konter, Oliver Esper, Jan Liebhold, Andrew Kyncl, Tomas Schneider, Lea Duethorn, Elisabeth Buentgen, Ulf TI Tree-ring evidence for the historical absence of cyclic larch budmoth outbreaks in the Tatra Mountains SO TREES-STRUCTURE AND FUNCTION LA English DT Article DE Dendroecology; Forest entomology; Host abundance; Paleoclimatology; Population ecology; Trophic interaction ID BUD-MOTH CYCLE; ZEIRAPHERA-DINIANA; POPULATION FLUCTUATIONS; INSECT OUTBREAKS; TIME-SERIES; HOST RACES; CLIMATE; RECONSTRUCTIONS; ALPS AB Key message The absence of larch budmoth outbreaks and subsequent consequences on tree rings together with a distinct climate-growth relationship enhance the dendroclimatic potential of larch ring width data from the Tatra Mountains. Regular population oscillations are generally considered to arise from trophic interactions, though it is unclear how such cycles are affected by biotic and abiotic factors. Cyclic outbreaks of the larch budmoth (LBM; Zeiraphera diniana), perhaps the most prominent example of periodic insect population dynamics, leave distinct "fingerprints' in the annual rings of host trees, and have been reconstructed over 1,200 years in the European Alps. Although LBM individuals are known to exist in other regions, it is unclear whether recurrent mass outbreaks historically occurred elsewhere. Here, we present new larch (Larix decidua) host and pine (Pinus cembra) non-host chronologies from the Slovakian Tatra that comprise 323 ring width samples dating back to 1612 AD. May-June and May-July temperatures control larch and pine growth (r(1951-2011) = 0.63 and 0.57; p < 0.001), respectively. LBM outbreak-induced defoliation patterns and subsequent ring width reductions were absent over the past three centuries, during which larch (host) and pine (non-host) growth was significantly synchronized (r(1725-2012) = 0.48; p < 0.001). Spatially limited host forests of overall low stand densities along the northwestern Carpathian arc together with a relatively warm climate envelope are most likely responsible for the absence of cyclic LBM outbreaks. Tree-ring chronologies from these ecotones, free of pulsed disruptions, therefore, represent unique paleoclimatic archives ideal for reconstructing interannual to multi-centennial variations in Eastern European summer temperature. C1 [Konter, Oliver; Esper, Jan; Schneider, Lea; Duethorn, Elisabeth] Johannes Gutenberg Univ Mainz, Dept Geog, D-55099 Mainz, Germany. [Liebhold, Andrew] US Forest Serv, No Res Stn, USDA, Morgantown, WV USA. [Kyncl, Tomas] Moravian Dendro Labor, Brno, Czech Republic. [Buentgen, Ulf] Swiss Fed Res Inst WSL, Birmensdorf, Switzerland. [Buentgen, Ulf] Oeschger Ctr Climate Change Res, Bern, Switzerland. [Buentgen, Ulf] Global Change Res Ctr AS CR, Brno, Czech Republic. RP Konter, O (reprint author), Johannes Gutenberg Univ Mainz, Dept Geog, Becherweg 21, D-55099 Mainz, Germany. EM o.konter@geo.uni-mainz.de RI buentgen, ulf/J-6952-2013 FU Operational Programme of Education for Competitiveness of Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.07/2.3.00/20.0248] FX Ulf Buntgen was supported by the Operational Programme of Education for Competitiveness of Ministry of Education, Youth and Sports of the Czech Republic (Project: Building up a multidisciplinary scientific team focused on drought, No. CZ.1.07/2.3.00/20.0248). Dr. Peter Fleischer from the State forests of TANAP kindly organized and contributed to fieldwork. We thank Marek Turcani for information about historical occurrence of insect outbreaks in the Tatra Mountains. NR 29 TC 2 Z9 2 U1 3 U2 22 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0931-1890 EI 1432-2285 J9 TREES-STRUCT FUNCT JI Trees-Struct. Funct. PD JUN PY 2015 VL 29 IS 3 BP 809 EP 814 DI 10.1007/s00468-015-1160-0 PG 6 WC Forestry SC Forestry GA CI1EF UT WOS:000354484000017 ER PT J AU Elliott, TF Trappe, JM Weise, A AF Elliott, Todd F. Trappe, James M. Weise, Armin TI Australasian Sequestrate Fungi 19: Hysterangium colossum sp nov. SO IMA FUNGUS LA English DT Article DE Basidiomycota; Hysterangiales; Hysterangiaceae hypogeous fungus; Eucalyptus mycorrhizal fungus AB Hysterangium colossum sp. nov., with extraordinarily large basidiomata for the genus, is described from dry Eucalyptus woodlands in the Australian Capital Territory and southeastern New South Wales. It typically grows in confluent clusters and has a thick peridium often invaginated into the gleba. C1 [Elliott, Todd F.] Warren Wilson Coll, Dept Integrat Studies, POB 9000, Asheville, NC 28815 USA. [Trappe, James M.] Oregon State Univ, Dept Forest Sci, Corvallis, OR 97331 USA. [Trappe, James M.] US Forest Serv, Pacific Northwest Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. [Weise, Armin] Warren Wilson Coll, Dept Environm Studies, Asheville, NC 28815 USA. RP Elliott, TF (reprint author), Warren Wilson Coll, Dept Integrat Studies, POB 9000, Asheville, NC 28815 USA. EM toddfelliott@gmail.com NR 4 TC 0 Z9 0 U1 1 U2 5 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2015 VL 6 IS 1 BP 115 EP 117 DI 10.5598/imafungus.2015.06.01.05 PG 3 WC Mycology SC Mycology GA V46UL UT WOS:000209909000017 PM 26203416 ER PT J AU Rossman, AY Adams, GC Cannon, PF Castlebury, LA Crous, PW Gryzenhout, M Jaklitsch, WM Mejia, LC Stoykov, D Udayanga, D Voglmayr, H Walker, DM AF Rossman, Amy Y. Adams, Gerard C. Cannon, Paul F. Castlebury, Lisa A. Crous, Pedro W. Gryzenhout, Marieka Jaklitsch, Walter M. Mejia, Luis C. Stoykov, Dmitar Udayanga, Dhanushka Voglmayr, Hermann Walker, Donald M. TI Recommendations of generic names in Diaporthales competing for protection or use SO IMA FUNGUS LA English DT Article DE Article 59; Ascomycetes; Fungi; nomenclature; one fungus-one name; pleomorphic fungi; taxonomy; unit nomenclature AB In advancing to one name for fungi, this paper treats generic names competing for use in the order Diaporthales (Ascomycota, Sordariomycetes) and makes a recommendation for the use or protection of one generic name among synonymous names that may be either sexually or asexually typified. A table is presented that summarizes these recommendations. Among the genera most commonly encountered in this order, Cytospora is recommended over Valsa and Diaporthe over Phomopsis. New combinations are introduced for the oldest epithet of important species in the recommended genus. These include Amphiporthe tiliae, Coryneum lanciforme, Cytospora brevispora, C. ceratosperma, C. cinereostroma, C. eugeniae, C. fallax, C. myrtagena, Diaporthe amaranthophila, D. annonacearum, D. bougainvilleicola, D. caricae- papayae, D. cocoina, D. cucurbitae, D. juniperivora, D. leptostromiformis, D. pterophila, D. theae, D. vitimegaspora, Mastigosporella georgiana, Pilidiella angustispora, P. calamicola, P. pseudogranati, P. stromatica, and P. terminaliae. C1 [Rossman, Amy Y.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Adams, Gerard C.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68503 USA. [Cannon, Paul F.] Royal Bot Gardens, Surrey TW9 3AB, England. [Castlebury, Lisa A.; Udayanga, Dhanushka] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Crous, Pedro W.] CBS KNAW Fungal Biodivers Inst, NL-3584 CT Utrecht, Netherlands. [Gryzenhout, Marieka] Univ Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. [Jaklitsch, Walter M.] Univ Vienna, Dept Bot & Biodivers Res, Div Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Jaklitsch, Walter M.] BOKU Univ Nat Resources & Life Sci, Dept Forest & Soil Sci, Inst Forest Entomol Forest Pathol & Forest, A-1190 Vienna, Austria. [Mejia, Luis C.] Inst Sci Res & High Technol Serv INDICASAT AIP, Ctr Cellular & Mol Biol Dis, Panama City, Panama. [Mejia, Luis C.] Smithsonian Trop Res Inst, Balboa, Panama. [Stoykov, Dmitar] Bulgarian Acad Sci, Inst Biodivers & Ecosyst Res, Dept Plant & Fungal Divers & Resources, Sofia 113, Bulgaria. [Voglmayr, Hermann] Univ Vienna, Dept Bot & Biodivers Res, Div Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Walker, Donald M.] Findlay Univ, Dept Nat Sci, Findlay, OH 45840 USA. RP Rossman, AY (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM amydianer@yahoo.com FU Austrian Science Fund (FWF) [P27645-B16] FX Hermann Voglmayr acknowledges financial support by the Austrian Science Fund (FWF; project P27645-B16). NR 64 TC 15 Z9 15 U1 2 U2 6 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2015 VL 6 IS 1 BP 145 EP 154 DI 10.5598/imafungus.2015.06.01.09 PG 10 WC Mycology SC Mycology GA V46UL UT WOS:000209909000021 PM 26203420 ER PT J AU Wingfield, BD Ades, PK Al-Naemi, FA Beirn, LA Bihon, W Crouch, JA de Beer, ZW De Vos, L Duong, TA Fields, CJ Fourie, G Kanzi, AM Malapi-Wight, M Pethybridge, SJ Radwan, O Rendon, G Slippers, B Santana, QC Steenkamp, ET Taylor, PWJ Vaghefi, N van der Merwe, NA Veltri, D Wingfield, MJ AF Wingfield, Brenda D. Ades, Peter K. Al-Naemi, Fatima A. Beirn, Lisa A. Bihon, Wubetu Crouch, Jo Anne de Beer, Z. Wilhelm De Vos, Lieschen Duong, Tuan A. Fields, Christopher J. Fourie, Gerda Kanzi, Aquillah M. Malapi-Wight, Martha Pethybridge, Sarah J. Radwan, Osman Rendon, Gloria Slippers, Bernard Santana, Quentin C. Steenkamp, Emma T. Taylor, Paul W. J. Vaghefi, Niloofar van der Merwe, Nicolaas A. Veltri, Daniel Wingfield, Michael J. TI Draft genome sequences of Chrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygamai, Leptographium lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, and Thielaviopsis punctulata SO IMA FUNGUS LA English DT Article DE basal stalk and root rot; black scorch disease; blue stain; canker pathogen; pink patch disease; ray blight AB The genomes of Chrysoporthe austroafricana, Diplodia scrobiculata, Fusarium nygami, Leptographium lundbergii, Limonomyces culmigenus, Stagonosporopsis tanaceti, and Thielaviopsis punctulata are presented in this genome announcement. These seven genomes are from endophytes, plant pathogens and economically important fungal species. The genome sizes range from 26.6 Mb in the case of Leptographium lundbergii to 44 Mb for Chrysoporthe austroafricana. The availability of these genome data will provide opportunities to resolve longstanding questions regarding the taxonomy of species in these genera, and may contribute to our understanding of the lifestyles through comparative studies with closely related organisms. C1 [Wingfield, Brenda D.; De Vos, Lieschen; Duong, Tuan A.; Kanzi, Aquillah M.; Slippers, Bernard; Santana, Quentin C.; van der Merwe, Nicolaas A.] Univ Pretoria, Forestry & Agr Biotechnol Inst, Dept Genet, P Bag X20, ZA-0028 Pretoria, South Africa. [Ades, Peter K.] Univ Melbourne, Dept Forest & Ecosyst Sci, Melbourne, Vic 3010, Australia. [Al-Naemi, Fatima A.] Qatar Univ, Coll Arts & Sci, Dept Biol & Environm Sci, Doha, Qatar. [Beirn, Lisa A.] Rutgers State Univ, Dept Plant Biol & Pathol, 59 Dudley Rd, New Brunswick, NJ 08901 USA. [Bihon, Wubetu; de Beer, Z. Wilhelm; Fourie, Gerda; Steenkamp, Emma T.; Wingfield, Michael J.] Univ Pretoria, Forestry & Agr Biotechnol Inst, Dept Microbiol & Plant Pathol, ZA-0028 Pretoria, South Africa. [Bihon, Wubetu] Agr Res Council, Vegetable & Ornamental Plant Inst, ZA-0001 Pretoria, South Africa. [Crouch, Jo Anne; Malapi-Wight, Martha] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. [Fields, Christopher J.; Rendon, Gloria] Univ Illinois, Roy J Carver Biotechnol Ctr, WM Keck Ctr, High Performance Biol Comp Grp, Champaign, IL USA. [Pethybridge, Sarah J.; Vaghefi, Niloofar] Cornell Univ, Pathol & Plant Microbe Biol Sect, Sch Integrat Plant Sci, Geneva, NY 14456 USA. [Radwan, Osman] Univ Illinois, Dept Nat Resources & Environm Sci, Champaign, IL USA. [Radwan, Osman] Zagazig Univ, Coll Technol, Dept Plant Prod, Sharkia, Egypt. [Taylor, Paul W. J.] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia. [Veltri, Daniel] USDA ARS, Oak Ridge Labs, ARS Res Participat Program, Beltsville, MD 20705 USA. RP Wingfield, BD (reprint author), Univ Pretoria, Forestry & Agr Biotechnol Inst, Dept Genet, P Bag X20, ZA-0028 Pretoria, South Africa. EM brenda.wingfield@fabi.up.ac.za RI Slippers, Bernard/A-9351-2008; Wingfield, Michael/A-9473-2008; Wingfield, Brenda/B-1376-2008; De Vos, Lieschen/G-2328-2016; Steenkamp, Emma/B-7958-2009; de Beer, Z. Wilhelm/B-6353-2008; OI Slippers, Bernard/0000-0003-1491-3858; Wingfield, Brenda/0000-0002-6189-1519; Steenkamp, Emma/0000-0003-0217-8219; de Beer, Z. Wilhelm/0000-0001-9758-8987; Malapi-Wight, Martha/0000-0003-2249-6257; van der Merwe, Albe/0000-0003-3185-348X FU Genomics Research Institute (University of Pretoria); DST-NRF Centre of Excellence in Tree Health Biotechnology (CTHB) at the Forestry and Agricultural Biotechnology Institute (FABI); National Research Foundation (NRF); University of Pretoria, Pretoria, South Africa; Botanical Resources Australia, Agricultural Services Pty Ltd.; Agricultural Services Pty Ltd.; USDA-NIFA Hatch project [NYG-625424]; US Department of Agriculture (USDA), Agricultural Research Service (ARS); USDA-ARS Headquarters Research Associate Award; ARS Research Participation Program; ORAU under DOE [DE-AC05-06OR23100]; Qatar National Research Fund (QNRF) under National Priorities Research Program [NPRP-5-1002-4-010]; Claude Leon Foundation, South Africa FX The genome sequencing of Diplodia scrobiculata, Chrysoporthe austroafricana, Fusarium nygamai, Leptographium lundbergii was co-funded by the Genomics Research Institute (University of Pretoria), Tree Protection Co-operative Programme (TPCP) and the DST-NRF Centre of Excellence in Tree Health Biotechnology (CTHB) at the Forestry and Agricultural Biotechnology Institute (FABI), together with the National Research Foundation (NRF) and the University of Pretoria, Pretoria, South Africa. W.B. is grateful to the Claude Leon Foundation, South Africa for a Post-Doctoral Fellowship. The research on Stagonosporopsis tanaceti was supported by Botanical Resources Australia, Agricultural Services Pty Ltd., and USDA-NIFA Hatch project NYG-625424, managed by The New York Agricultural Experiment Station, Cornell University (Geneva, NY).; The genome sequencing of Limonomyces culmigenus (CBS 661.85) was funded by the US Department of Agriculture (USDA), Agricultural Research Service (ARS). M.M.W. is supported by a Class of 2013 USDA-ARS Headquarters Research Associate Award to J.A.C. This research was supported in part by the appointment of D.V. to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the US Department of Energy (DOE) and the USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. All opinions expressed in this paper are the author's and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer.; The research on Thielaviopsis punctulata was made possible by a grant from the Qatar National Research Fund (QNRF) under National Priorities Research Program (NPRP-5-1002-4-010). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the QNRF. We thank Alvaro Hernandez (Roy J. Carver Biotechnology Center/W.M. Keck Center, University of Illinois at Urbana-Champaign, IL) for generation of the sequence libraries used for this genome assembly. We also would like to thank Arshdeep Saroa (Department of Natural Resources and Environmental Sciences, University of Illinois at Urbana-Champaign, IL) for technical assistance in this project. NR 141 TC 6 Z9 6 U1 0 U2 5 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2015 VL 6 IS 1 BP 233 EP 248 DI 10.5598/imafungus.2015.06.01.15 PG 16 WC Mycology SC Mycology GA V46UL UT WOS:000209909000027 PM 26203426 ER PT J AU Meter, K AF Meter, Ken TI Allowing ethnic heritage to emerge in farm and food policy SO Journal of Agriculture Food Systems and Community Development LA English DT Editorial Material C1 [Meter, Ken] Agr Mkt Serv, USDA, Econ Impacts Local & Reg Food Syst Toolkit, Washington, DC 20250 USA. RP Meter, K (reprint author), Agr Mkt Serv, USDA, Econ Impacts Local & Reg Food Syst Toolkit, Washington, DC 20250 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU NEW LEAF ASSOC, INC PI ITHACA PA 295 HOOK PL, ITHACA, NY 14850 USA SN 2152-0798 EI 2152-0801 J9 J AGRIC FOOD SYST CO JI J. Agric. Food Syst. Community Dev. PD SUM PY 2015 VL 5 IS 4 BP 11 EP 13 DI 10.5304/jafscd.2015.054.032 PG 3 WC Agronomy SC Agriculture GA ED9XU UT WOS:000389227700003 ER PT J AU Bengston, DN Olson, RL AF Bengston, David N. Olson, Robert L. TI Asynchronous Online Foresight Panels: The Case of Wildfire Management SO JOURNAL OF FUTURES STUDIES LA English DT Article DE Foresight panel; asynchronous online conferencing; wildfire management ID DELPHI AB Text-based asynchronous online conferencing involves structured online discussion and deliberation among multiple participants from multiple sites in which there is a delay in interaction between contributors. This method has been widely used for a variety of purposes in higher education and other settings, but has not been commonly used in futures research. This paper describes an asynchronous online foresight panel process. The method is illustrated with the case of a recent foresight panel on the future of wildland fire management. C1 US Forest Serv, St Paul, MN 55108 USA. [Olson, Robert L.] Inst Alternat Futures, Alexandria, VA 22314 USA. RP Bengston, DN (reprint author), US Forest Serv, Environm Futurist Northern Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM dbengston@fs.fed.us; bobolson2020@gmail.com NR 32 TC 1 Z9 1 U1 0 U2 0 PU TAMKANG UNIV PI TAMSUI PA GRAD SCHOOL LANG LIT, TAMSUI, TAIPEI, TAIWAN SN 1027-6084 J9 J FUTURES STUD JI J. Futures Stud. PD JUN PY 2015 VL 19 IS 4 BP 1 EP 11 PG 11 WC Planning & Development SC Public Administration GA DP9SM UT WOS:000378837300001 ER PT J AU Hallema, DW Periard, Y Lafond, JA Gumiere, SJ Caron, J AF Hallema, Dennis W. Periard, Yann Lafond, Jonathan A. Gumiere, Silvio J. Caron, Jean TI Characterization of Water Retention Curves for a Series of Cultivated Histosols SO VADOSE ZONE JOURNAL LA English DT Article ID UNSATURATED HYDRAULIC CONDUCTIVITY; FUNCTION MINIMIZATION; PEAT SOILS; MOISTURE RETENTION; MODEL; ALGORITHMS AB Water retention curves are essential for the parameterization of soil water models such as HYDRUS. Although hydraulic parameters are known for a large number of mineral and natural organic soils, our knowledge on the hydraulic behavior of cultivated Histosols is rather limited. The objective of this study was to derive characteristic water retention curves for a large cultivated peatland with lettuce ( Lactuca sativa L.) and vegetable farming in southern Quebec, Canada. A comparison showed that the van Genuchten model fits better to the water retention data obtained with a Tempe pressure cell experiment than the Groenevelt-Grant model in terms of residual sum of squares; however, the difference in performance was quite small due to the high number of iterations used for fitting. Finally, an agglomerative cluster analysis of 85 peat samples allowed us to define two distinct water retention curves, where the first water retention curve described samples of relatively shallow (< 150 cm) Histosols with an organic content < 0.89 and a bulk density > 0.3 g cm(-3), and the second curve characterized samples of the deepest ( depth 150-230 cm) Histosols with an organic content of up to 0.97 and a bulk density > 0.3 g cm(-3), which are the soils that suffered a more dramatic transformation as a result of agriculture. This characterization allows for a multitude of applications, including parameterization of the HYDRUS model for soil water movement, and presents an essential tool for the optimization of water management in cultivated peatlands. C1 [Hallema, Dennis W.; Periard, Yann; Lafond, Jonathan A.; Gumiere, Silvio J.; Caron, Jean] Univ Laval, Dept Sols & Genie Agroalimentaire, Quebec City, PQ G1V 0A6, Canada. [Hallema, Dennis W.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. RP Hallema, DW (reprint author), Univ Laval, Dept Sols & Genie Agroalimentaire, 2480 Blvd Hochelaga, Quebec City, PQ G1V 0A6, Canada. EM dwhallem@ncsu.edu FU Natural Sciences and Engineering Research Council of Canada; Delfland Inc.; Les Fermes Hotte et Van Winden Inc.; Maraichers J.P.L. Guerin et Fils Inc.; Production Horticole Van Winden Inc.; Vert Nature Inc. FX We want to thank the Natural Sciences and Engineering Research Council of Canada and our farming partners, Delfland Inc., Les Fermes Hotte et Van Winden Inc., Maraichers J.P.L. Guerin et Fils Inc., Production Horticole Van Winden Inc., and Vert Nature Inc., for funding the research that resulted in this paper. Our gratitude extends to G. Sun, G. Sauvageau, and S.C. Vanlandeghem for their respective contributions. NR 38 TC 2 Z9 2 U1 2 U2 2 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD JUN PY 2015 VL 14 IS 6 DI 10.2136/vzj2014.10.0148 PG 8 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CS4RZ UT WOS:000362064900011 ER PT J AU Hallema, DW Lafond, JA Periard, Y Gumiere, SJ Sun, G Caron, J AF Hallema, Dennis W. Lafond, Jonathan A. Periard, Yann Gumiere, Silvio J. Sun, Ge Caron, Jean TI Long-Term Effects of Peatland Cultivation on Soil Physical and Hydraulic Properties: Case Study in Canada SO VADOSE ZONE JOURNAL LA English DT Article ID WATER MANAGEMENT; ORGANIC SOILS; SPATIAL INTERPOLATION; FIELD; EVAPOTRANSPIRATION; CONDUCTIVITY; LETTUCE; QUEBEC AB Organic soils are an excellent substrate for commercial lettuce (Lactuca sativa L.) farming; however, drainage accelerates oxidation of the surface layer and reduces the water holding capacity, which is often lethal for crops that are sensitive to water stress. In this case study, we analyzed 942 peat samples from a large cultivated peatland complex (18.7 km(2)) in southern Quebec, Canada, and demonstrated from spatial and temporal patterns that agriculture resulted in a compacted layer below the root zone. We grouped the samples based on the year in which the corresponding fields were created on the previously undisturbed peatland (cutoff years 1970, 1980, 1990, and 2000) and discovered that bulk density has continued to increase, partly due to the overburden pressure, while organic matter has continued to decline since the fields were reclaimed and drained in phases between 1955 and 2006. Saturated hydraulic conductivity (K-s) in the upper 20 cm was remarkably lower on fields older than 10 yr (p = 0.0973 for Wilcoxon rank test), with more samples having a Ks < 2.0 x 10(-3) yr. Soil water available capacity (SWAC) was between approximately 5 and 33 cm on fields reclaimed after 2000, while samples from fields reclaimed before 2000 had a lower SWAC between 2 and 23 cm (groups discernable at p = 0.0203). It is possible, however, that the greatest rate of change in Ks and SWAC occurred within even a year of reclamation. The results of this study call for active measures to reduce organic soil degradation such as reducing tillage and on-field traffic or following a crop rotation scheme. C1 [Hallema, Dennis W.; Lafond, Jonathan A.; Periard, Yann; Gumiere, Silvio J.; Caron, Jean] Univ Laval, Dept Sols & Genie Agroalimentaire, Quebec City G1V 0A6, PQ, Canada. [Hallema, Dennis W.; Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC 27606 USA. RP Hallema, DW (reprint author), Univ Laval, Dept Sols & Genie Agroalimentaire, 2480 Blvd Hochelaga, Quebec City G1V 0A6, PQ, Canada. EM dwhallem@ncsu.edu FU Natural Sciences and Engineering Research Council of Canada; Delfland Inc.; Les Fermes Hotte et Van Winden Inc.; Maraichers J.P.L. Guerin et Fils Inc.; Production Horticole Van Winden Inc.; Vert Nature Inc. FX We want to thank the Natural Sciences and Engineering Research Council of Canada and our farming partners Delfland Inc., Les Fermes Hotte et Van Winden Inc., Maraichers J.P.L. Guerin et Fils Inc., Production Horticole Van Winden Inc., and Vert Nature Inc. for funding the research that resulted in this paper. Our gratitude extends to P.H. Hiemstra for his advice on TPS interpolation, and we also thank S. Boudreault, J. Corriveau Boulay, S. Jutras, P.Y. Pettigrew-Blanchet, V. Premont, G. Sauvageau, and S.C. Vanlandeghem for their respective contributions. NR 36 TC 2 Z9 2 U1 2 U2 2 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD JUN PY 2015 VL 14 IS 6 DI 10.2136/vzj2014.10.0147 PG 12 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CS4RZ UT WOS:000362064900010 ER PT J AU Sun, J Sonstegard, TS Li, C Huang, Y Li, Z Lan, X Zhang, C Lei, C Zhao, X Chen, H AF Sun, J. Sonstegard, T. S. Li, C. Huang, Y. Li, Z. Lan, X. Zhang, C. Lei, C. Zhao, X. Chen, H. TI Altered microRNA expression in bovine skeletal muscle with age SO ANIMAL GENETICS LA English DT Article DE ANGPT1; growth and development; miRNA target prediction ID TARGET RECOGNITION; AXON GUIDANCE; DIFFERENTIATION; PROLIFERATION; MIR-206; CELLS; TRANSCRIPTION; RESTRICTION; MYOGENESIS; SARCOPENIA AB Age-dependent decline in skeletal muscle function leads to several inherited and acquired muscular disorders in elderly individuals. The levels of microRNAs (miRNAs) could be altered during muscle maintenance and repair. We therefore performed a comprehensive investigation for miRNAs from five different periods of bovine skeletal muscle development using next-generation small RNA sequencing. In total, 511 miRNAs, including one putatively novel miRNA, were identified. Thirty-six miRNAs were differentially expressed between prenatal and postnatal stages of muscle development including several myomiRs (miR-1, miR-206 and let-7 families). Compared with miRNA expression between different muscle tissues, 14 miRNAs were up-regulated and 22 miRNAs were down-regulated in the muscle of postnatal stage. In addition, a novel miRNA was predicted and submitted to the miRBase database as bta-mir-10020. A dual luciferase reporter assay was used to demonstrate that bta-mir-10020 directly targeted the 3-UTR of the bovine ANGPT1 gene. The overexpression of bta-mir-10020 significantly decreased the DsRed fluorescence in the wild-type expression cassette compared to the mutant type. Using three computational approaches - miranda, pita and rnahybrid - these differentially expressed miRNAs were also predicted to target 3609 bovine genes. Disease and biological function analyses and the KEGG pathway analysis revealed that these targets were statistically enriched in functionality for muscle growth and disease. Our miRNA expression analysis findings from different states of muscle development and aging significantly expand the repertoire of bovine miRNAs now shown to be expressed in muscle and could contribute to further studies on growth and developmental disorders in this tissue type. C1 [Sun, J.; Huang, Y.; Li, Z.; Lan, X.; Lei, C.; Zhao, X.; Chen, H.] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. [Sun, J.; Sonstegard, T. S.; Li, C.] USDA ARS, Anim Genom & Improvement Lab, BARC East, Beltsville, MD 20705 USA. [Zhang, C.] Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China. [Zhao, X.] McGill Univ, Dept Anim Sci, Ste Anne De Bellevue, PQ H9X 3V9, Canada. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. EM chenhong1212@263.net FU National Natural Science Foundation of China [31272408]; National 863 Program of China [2013AA102505]; Program of National Beef Cattle Industrial Technology System [CARS-38] FX This study was supported by the National Natural Science Foundation of China (Grant No. 31272408), National 863 Program of China (Grant No. 2013AA102505) and the Program of National Beef Cattle Industrial Technology System (CARS-38). NR 57 TC 4 Z9 4 U1 0 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD JUN PY 2015 VL 46 IS 3 BP 227 EP 238 DI 10.1111/age.12272 PG 12 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CH4HX UT WOS:000353995700001 PM 25703017 ER PT J AU Dassanayake, RP White, SN Madsen-Bouterse, SA Schneider, DA O'Rourke, KI AF Dassanayake, Rohana P. White, Stephen N. Madsen-Bouterse, Sally A. Schneider, David A. O'Rourke, Katherine I. TI Role of the PRNP S127 allele in experimental infection of goats with classical caprine scrapie SO ANIMAL GENETICS LA English DT Editorial Material ID EXPERIMENTALLY CHALLENGED GOATS; GENE; RESISTANCE; BREEDS C1 [Dassanayake, Rohana P.; White, Stephen N.; Madsen-Bouterse, Sally A.; Schneider, David A.; O'Rourke, Katherine I.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [White, Stephen N.; Schneider, David A.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Dassanayake, RP (reprint author), Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM rohana1@vetmed.wsu.edu NR 8 TC 3 Z9 4 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD JUN PY 2015 VL 46 IS 3 BP 341 EP 341 DI 10.1111/age.12291 PG 1 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CH4HX UT WOS:000353995700019 PM 25917307 ER PT J AU Dong, SZ Zhang, HW Chen, X Stanley, D Yu, XP Song, QS AF Dong, Shengzhang Zhang, Hongwei Chen, Xi Stanley, David Yu, Xiaoping Song, Qisheng TI THE NEUROPEPTIDE BURSICON ACTS IN CUTICLE METABOLISM SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE bursicon; cuticular protein; chitin-binding protein; transcriptome ID DROSOPHILA-MELANOGASTER; RECEPTOR; HORMONE; BEHAVIOR AB Bursicon is a heterodimeric neuropeptide formed of bursicon alpha (burs alpha) and bursicon beta (burs beta) that controls cuticle tanning and wing expansion in insects. Burs alpha-alpha and burs beta-beta homodimers are also formed; they act via an unknown receptor to induce expression of prophylactic immune and stress genes during molting. Based on the hypothesis that burs beta-beta and/or bursicon influence expression of additional genes acting after the molt, we prepared and sequenced six Drosophila cDNA libraries from groups of flies separately injected with burs beta-beta, bursicon, or blank control. Compared to the control, the burs beta-beta treatments led to upregulation (by at least 1.5-fold) of 262 genes at 0.5 h postinjection (PI) and 298 genes at 1 h PI; 323 genes at 0.5 h PI and 269 genes at 1h PI were downregulated (by at least 0.67). Similar changes were recorded following bursicon injections. Of these genes, expression of seven transcripts encoding cuticle proteins was upregulated and three downregulated by burs beta-beta; expression of nine transcripts encoding cuticle proteins were upregulated and four downregulated following bursicon treatments. Expression of dozens of genes involved in chitin metabolism was altered by the experimental treatments. We recorded parallel changes in expression of selected genes by transcriptome and qPCR analysis. These findings support our hypothesis that burs beta-beta and bursicon influence expression of additional genes acting after the molt. We report that burs beta-beta and bursicon act in cuticle synthesis and degradation by regulating the expression of cuticular protein and chitin metabolizing related genes. (C) 2015 Wiley Periodicals, Inc. C1 [Dong, Shengzhang; Zhang, Hongwei; Chen, Xi; Song, Qisheng] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Dong, Shengzhang; Yu, Xiaoping] China Jiliang Univ, Coll Life Sci, Zhejiang Prov Key Lab Biometrol & Inspect & Quara, Hangzhou, Zhejiang, Peoples R China. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. RP Song, QS (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM songq@missouri.edu OI Dong, Shengzhang/0000-0002-8218-4688 NR 17 TC 3 Z9 3 U1 3 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD JUN PY 2015 VL 89 IS 2 BP 87 EP 97 DI 10.1002/arch.21227 PG 11 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA CH9RK UT WOS:000354373700003 PM 25821138 ER PT J AU Stoof, CR Richards, BK Woodbury, PB Fabio, ES Brumbach, AR Cherney, J Das, S Geohring, L Hansen, J Hornesky, J Mayton, H Mason, C Ruestow, G Smart, LB Volk, TA Steenhuis, TS AF Stoof, Cathelijne R. Richards, Brian K. Woodbury, Peter B. Fabio, Eric S. Brumbach, Alice R. Cherney, Jerry Das, Srabani Geohring, Larry Hansen, Julie Hornesky, Josh Mayton, Hilary Mason, Cedric Ruestow, Gerry Smart, Lawrence B. Volk, Timothy A. Steenhuis, Tammo S. TI Untapped Potential: Opportunities and Challenges for Sustainable Bioenergy Production from Marginal Lands in the Northeast USA SO BIOENERGY RESEARCH LA English DT Article DE Second-generation bioenergy feedstocks; Marginal land; Perennial grass; Short-rotation woody crops; Impacts; Production; Policy; Northeast USA ID SOIL ORGANIC-CARBON; SWITCHGRASS PANICUM-VIRGATUM; CONTERMINOUS UNITED-STATES; SHORT-ROTATION PLANTATIONS; SCALE COMBUSTION SYSTEMS; REPUBLIC-OF-GERMANY; FORMER ARABLE LAND; BIOMASS PRODUCTION; ENERGY CROPS; NEW-YORK AB Over two million hectares of marginal land in the Northeast USA no longer used for agriculture may be suitable and available for production of second-generation cellulosic bioenergy crops, offering the potential for increased regional bioenergy production without competing with food production on prime farmland. Current yields of perennial bioenergy grasses and short-rotation woody crops range from 2.3 to 17.4 and 4.5 to 15.5 Mg/ha, respectively, and there is great potential for increased yields. Regional advantages for bioenergy development include abundant water resources, close proximity between production and markets, and compatibility of bioenergy cropping systems with existing agriculture. As New York and New England (a subset of the Northeast region) account for similar to 85 % of the nation's heating oil consumption, production of bioheat, biopower, and combined heat and power could substantially reduce the region's dependence on imported petroleum. While numerous grassroots efforts are underway in the region across supply chains, bioenergy development faces several challenges and unknowns in terms of environmental impact, production, yields, socioeconomics, and policy. We explore the opportunities for second-generation bioenergy production on the unused marginal lands of the Northeast USA and discuss the challenges to be addressed to promote sustainable bioenergy production on the region's underutilized marginal land base. C1 [Stoof, Cathelijne R.; Richards, Brian K.; Das, Srabani; Geohring, Larry; Mason, Cedric; Steenhuis, Tammo S.] Cornell Univ, Dept Biol & Environm Engn, Ithaca, NY 14853 USA. [Woodbury, Peter B.; Cherney, Jerry] Cornell Univ, Soil & Crop Sci Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Fabio, Eric S.; Smart, Lawrence B.] Cornell Univ, Sch Integrat Plant Sci, Hort Sect, New York State Agr Expt Stn, Geneva, NY 14456 USA. [Brumbach, Alice R.] New York Bioenergy Assoc, Rensselaer, NY 12144 USA. [Hansen, Julie; Mayton, Hilary] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Hornesky, Josh] Nat Resources Conservat Serv, USDA, Mexico, NY USA. [Mayton, Hilary] Cornell Univ, Ctr Teaching Excellence, Ithaca, NY 14853 USA. [Ruestow, Gerry] Fermata Consulting, Unadilla, NY 13849 USA. [Volk, Timothy A.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. RP Stoof, CR (reprint author), Wageningen Univ, Soil Geog & Landscape Grp, POB 47, NL-6700 AA Wageningen, Netherlands. EM cathelijne.stoof@wur.nl; bkr2@cornell.edu OI Woodbury, Peter/0000-0003-3954-7639 FU USDA-NIFA [2010-03869]; AFRI [2012-68005-19703]; Cornell University Agricultural Experiment Station [125-7832]; USDOE Energy Bioenergy Technologies Office through the North Central Regional Sun Grant Center at South Dakota State University [DE-FC36-05GO85041] FX This paper arose from a collaborative multidisciplinary seminar series "Untapped Potential: Sustainable Bioenergy Production on Marginal Lands of New York and the Northeast" held at Cornell University in 2012, 2013, and 2014. The seminar brought together speakers from inside and outside of academia, focusing on the region's marginal land bioenergy potential. We thank all seminar participants for discussion, and Ellen Demey and Valerie Podolec for their helpful comments. This work was supported in part by the USDA-NIFA through Sustainable Bioenergy Grant No. 2010-03869, AFRI Competitive Grant No. 2012-68005-19703, and Cornell University Agricultural Experiment Station federal formula funds Project No. 125-7832; as well as by the USDOE Energy Bioenergy Technologies Office through the North Central Regional Sun Grant Center at South Dakota State University (Award No. DE-FC36-05GO85041). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the USDA, NIFA, USDA-NRCS, USDA-FSA, or the New York Bioenergy Association. NR 153 TC 7 Z9 7 U1 4 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2015 VL 8 IS 2 BP 482 EP 501 DI 10.1007/s12155-014-9515-8 PG 20 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA CH4UK UT WOS:000354028200002 ER PT J AU Murphy, KA Unruh, TR Zhou, LM Zalom, FG Shearer, PW Beers, EH Walton, VM Miller, B Chiu, JC AF Murphy, K. A. Unruh, T. R. Zhou, L. M. Zalom, F. G. Shearer, P. W. Beers, E. H. Walton, V. M. Miller, B. Chiu, J. C. TI Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE spotted wing drosophila; Drosophila suzukii; species-specific PCR; invasive pest; comparative genomics ID SPOTTED-WING DROSOPHILA; DIPTERA DROSOPHILIDAE; PCR; DNA; IMPROVEMENTS; INVASION; MARKERS AB Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species. C1 [Murphy, K. A.; Zhou, L. M.; Zalom, F. G.; Chiu, J. C.] Univ Calif Davis, Coll Agr & Environm Sci, Dept Entomol & Nematol, Davis, CA 95616 USA. [Unruh, T. R.] USDA ARS, Wapato, WA 98951 USA. [Shearer, P. W.] Oregon State Univ, Mid Columbia Agr & Extens Ctr, Hood River, OR 97031 USA. [Beers, E. H.] Washington State Univ, Tree Fruit Res & Extens Ctr, Wenatchee, WA 98801 USA. [Walton, V. M.; Miller, B.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Chiu, JC (reprint author), Univ Calif Davis, Coll Agr & Environm Sci, Dept Entomol & Nematol, Davis, CA 95616 USA. EM jcchiu@ucdavis.edu FU Washington Tree Fruit Research Commission; UC Davis Provost's Undergraduate Fellowship; McBeth Memorial Fellowship FX We thank the laboratories of David Begun (UC Davis Department of Evolution and Ecology), Artyom Kopp (UC Davis Department of Evolution and Ecology), and Kelly Hamby (University of Maryland) for sharing Drosophila strains. D. suzukii from Watsonville, CA, USA. were collected with permission by Garroutte Farms, Inc. (Watsonville, CA). We also thank Claudio loriatti from the Center for Technology Transfer, FEM-IASMA for D. suzukii collected from Italy. We thank Ernest K. Lee (AMNH, New York, NY) as well as the editor and reviewers for their valuable input into this manuscript. This project is supported by a Washington Tree Fruit Research Commission grant awarded to JCC and UC Davis Provost's Undergraduate Fellowship and McBeth Memorial Fellowship awarded to KAM. NR 22 TC 3 Z9 3 U1 2 U2 36 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD JUN PY 2015 VL 105 IS 3 BP 364 EP 372 DI 10.1017/S0007485315000218 PG 9 WC Entomology SC Entomology GA CH5VQ UT WOS:000354104000011 PM 25804294 ER PT J AU Hadziabdic, D Wadl, PA Staton, ME Klingeman, WE Moulton, JK Pscheidt, JW Wiggins, GJ Grant, JF Lambdin, PL Windham, MT Faccoli, M Merten, PR Trigiano, RN AF Hadziabdic, Denita Wadl, Phillip A. Staton, Margaret E. Klingeman, William E. Moulton, John K. Pscheidt, Jay W. Wiggins, Gregory J. Grant, Jerome F. Lambdin, Paris L. Windham, Mark T. Faccoli, Massimo Merten, Paul R. Trigiano, Robert N. TI Development of microsatellite loci in Pityophthorus juglandis, a vector of thousand cankers disease in Juglans spp. SO CONSERVATION GENETICS RESOURCES LA English DT Article DE Pityophthorus juglandis; Walnut twig beetle; Juglans cinerea; Juglans nigra; Thousand cankers disease; Microsatellite loci; Genetic diversity ID GEOSMITHIA-MORBIDA; CAUSAL AGENT; NIGRA AB Using next-generation sequencing, 18 microsatellite loci were developed and characterized for walnut twig beetle, Pityophthorus juglandis, a vector of thousand cankers disease (TCD) affecting Juglans spp. Although all Juglans species are susceptible to TCD infection, native populations of J. nigra and J. cinerea, which is endangered in Canada, are most susceptible and threatened by habitat loss. Novel primers amplified di-, tri-, and tetra nucleotide repeats and detected 4-14 alleles per locus. Averaged observed and expected heterozygosity was 0.22 and 0.67, respectively. Our results indicate that P. juglandis microsatellite loci can be used to investigate genetic diversity and population structure of this vector across a widespread geography. These markers will be useful tools for evaluating genetic structure of P. juglandis population outbreaks and developing appropriate conservation strategies. Microsatellite loci obtained in this study can also be utilized to determine relationships of P. juglandis to other closely related Pityophthorus spp. C1 [Hadziabdic, Denita; Wadl, Phillip A.; Staton, Margaret E.; Moulton, John K.; Wiggins, Gregory J.; Grant, Jerome F.; Lambdin, Paris L.; Windham, Mark T.; Trigiano, Robert N.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Klingeman, William E.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Pscheidt, Jay W.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Faccoli, Massimo] Univ Padua, Dept Agron Food Nat Resources Animals & Environm, I-35020 Legnaro, PD, Italy. [Merten, Paul R.] USDA Forest Serv, Forest Hlth Protect, Asheville, NC 28804 USA. RP Hadziabdic, D (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 370 Plant Biotechnol Bldg,2505 EJ Chapman Dr, Knoxville, TN 37996 USA. EM dhadziab@utk.edu FU USFS [13-DG-11083150-033]; USFS-Special Technology Development Program [13-DG-11083150-039] FX The authors thank the USFS (Grant # 13-DG-11083150-033) and USFS-Special Technology Development Program (Grant # 13-DG-11083150-039) for financial support, and Sujata Agarwal of the The University of Tennessee Genomics Hub for assistance with Ion Torrent PGM sequencing. NR 5 TC 2 Z9 2 U1 1 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-7252 EI 1877-7260 J9 CONSERV GENET RESOUR JI Conserv. Genet. Resour. PD JUN PY 2015 VL 7 IS 2 BP 431 EP 433 DI 10.1007/s12686-014-0388-0 PG 3 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA CH7JW UT WOS:000354213300029 ER PT J AU Martin, DE Latheef, MA Lopez, JD AF Martin, Daniel E. Latheef, Mohamed A. Lopez, Juan D. TI Evaluation of selected acaricides against twospotted spider mite (Acari: Tetranychidae) on greenhouse cotton using multispectral data SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Remote sensing; Spectral reflectance; Normalized difference vegetation index; Twospotted spider mite ID BIOLOGICAL EFFICIENCY; TRANSLAMINAR ACTIVITY; RESIDUAL ACTIVITY; INFESTED COTTON; AVERMECTIN B-1; URTICAE ACARI; SPRAY DRIFT; MORTALITY; ABAMECTIN; MODE AB Twospotted spider mite (TSSM), Tetranychus urticae Koch, is an early season pest of cotton in the mid-southern USA and causes reduction in yield, fiber quality and impaired seed germination. Objectives of this study were to investigate the efficacy of abamectin and spiromesifen with two divergent LC50 values against TSSM in a computer-operated spray table which simulated aerial application parameters. Combined with a pressure of 276 kPa and a speed of 8 km/h, a 650033 nozzle delivered a spray rate of 18.7 L/ha. The active ingredient rates were 1/8, 1/4, 1/2 and the lowest label recommended rates for early season cotton. The intent was to study efficacy relative to deposition characteristics at active ingredient rates equal to and lower than those recommended by the label. Spectral reflectance values from a multispectral optical sensor were used to calculate the Normalized Difference Vegetation Index which numerically described the surface reflectance characteristics of cotton canopies concomitant to damage caused by T. urticae in the greenhouse. Water sensitive paper samplers described spray droplet spectra parameters (D-v0.1, D-v0.5 and D-v0.9, A mu m) and percent spray coverage. The volume median diameter (D-v0.5, A mu m) for abamectin and spiromesifen were respectively, 218 and 258 at one-half rate of the lowest label rate. These spray droplets were well above the driftable portions of the spray volume (< 141 A mu m) for both abamectin and spiromesifen. Efficacy evaluations indicated that spiromesifen was more effective than abamectin in controlling T. urticae on early season cotton at one-half rate of the lowest label rate. Results reported herein demonstrate that the multispectral optical sensor in lieu of manually counting T. urticae appears to be a promising tool for efficacy evaluations against acaricides for early season plants grown in greenhouses. C1 [Martin, Daniel E.; Latheef, Mohamed A.; Lopez, Juan D.] USDA ARS, Aerial Applicat Technol Res Unit, College Stn, TX 77845 USA. RP Martin, DE (reprint author), USDA ARS, Aerial Applicat Technol Res Unit, College Stn, TX 77845 USA. EM daniel.martin@ars.usda.gov NR 67 TC 0 Z9 0 U1 3 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD JUN PY 2015 VL 66 IS 2 BP 227 EP 245 DI 10.1007/s10493-015-9903-6 PG 19 WC Entomology SC Entomology GA CH7CL UT WOS:000354193100005 PM 25863789 ER PT J AU Abdullaev, AA Salakhutdinov, IB Egamberdiev, SS Kuryazov, Z Glukhova, LA Adilova, AT Rizaeva, SM Ulloa, M Abdurakhmonov, IY AF Abdullaev, Alisher A. Salakhutdinov, Ilkhom B. Egamberdiev, Sharof Sh. Kuryazov, Zarif Glukhova, Ludmila A. Adilova, Azoda T. Rizaeva, Sofiya M. Ulloa, Mauricio Abdurakhmonov, Ibrokhim Y. TI Analyses of Fusarium wilt race 3 resistance in Upland cotton (Gossypium hirsutum L.) SO GENETICA LA English DT Article DE Fusarium; Wilt; Host-plant interaction; Resistance; Environment; Population; Disease severity ID F-SP VASINFECTUM; OXYSPORUM; INHERITANCE; CALIFORNIA; NEMATODES; TANZANIA; PLANTS AB Fusarium wilt [Fusarium oxysporum f.sp. vasinfectum (FOV) Atk. Sny & Hans] represents a serious threat to cotton (Gossypium spp.) production. For the last few decades, the FOV pathogen has become a significant problem in Uzbekistan causing severe wilt disease and yield losses of G. hirsutum L. cultivars. We present the first genetic analyses of FOV race 3 resistance on Uzbek Cotton Germplasm with a series of field and greenhouse artificial inoculation-evaluations and inheritance studies. The field experiments were conducted in two different sites: the experimental station in Zangiota region-Environment (Env) 1 and the Institute of Cotton Breeding (Env-2, Tashkent province). The Env-1 was known to be free of FOV while the Env-2 was known to be a heavily FOV infested soil. In both (Env-1 and Env-2) of these sites, field soil was inoculated with FOV race 3. F-2 and an F-3 Upland populations ("Mebane B1" x "11970") were observed with a large phenotypic variance for plant survival and FOV disease severity within populations and among control or check Upland accessions. Wilt symptoms among studied F-2 individuals and F-3 families significantly differed depending on test type and evaluation site. Distribution of Mendelian rations of susceptible (S) and resistant (R) phenotypes were 1S:1R field Env-1 and 3S:1R field Env-2 in the F-2 population, and 1S:3R greenhouse site in the F-3 population. The different segregation distribution of the Uzbek populations may be explained by differences in FOV inoculum level and environmental conditions during assays. However, genetic analysis indicated a recessive single gene action under high inoculum levels or disease pressure for FOV race 3 resistance. Uzbek germplasm may be more susceptible than expected to FOV race 3, and sources of resistance to FOV may be limited under the FOV inoculum levels present in highly-infested fields making the breeding process more complex. C1 [Abdullaev, Alisher A.; Salakhutdinov, Ilkhom B.; Egamberdiev, Sharof Sh.; Adilova, Azoda T.; Abdurakhmonov, Ibrokhim Y.] Minist Agr & Water Resources Uzbekistan, Acad Sci Uzbekistan, Ctr Genom & Bioinformat, Tashkent 111215, Qibray Region, Uzbekistan. [Abdullaev, Alisher A.; Salakhutdinov, Ilkhom B.; Egamberdiev, Sharof Sh.; Adilova, Azoda T.; Abdurakhmonov, Ibrokhim Y.] Uzpakhtasanoat Assoc, Tashkent Dist 111215, Qibray Region, Uzbekistan. [Abdullaev, Alisher A.; Kuryazov, Zarif; Glukhova, Ludmila A.; Rizaeva, Sofiya M.] Acad Sci Uzbek, Inst Genet & Plant Expt Biol, Tashkent 111226, Qibray Region, Uzbekistan. [Ulloa, Mauricio] ARS, USDA, PA, Cropping Syst Res Lab,Plant Stress & Germplasm De, Lubbock, TX 79415 USA. RP Abdurakhmonov, IY (reprint author), Minist Agr & Water Resources Uzbekistan, Acad Sci Uzbekistan, Ctr Genom & Bioinformat, Univ St 2, Tashkent 111215, Qibray Region, Uzbekistan. EM genomics@uzsci.net RI Abdurakhmonov, Ibrokhim/F-5588-2015 OI Abdurakhmonov, Ibrokhim/0000-0001-9563-0686 FU Office of International Research Programs, US Department of Agriculture (USDA) [UZB2-31016-TA-09] FX The authors thank the Office of International Research Programs, US Department of Agriculture (USDA) for funding this study under research grant UZB2-31016-TA-09 and U.S. Civilian Research & Development Foundation (CRDF) for project coordination. We thank also the Academy of Sciences of Uzbekistan and Committee for Coordination of Science and Technology Development of Uzbekistan, Cabinet of Ministers of the Republic of Uzbekistan for continual in-house support of the reserach projects. The USDA is an equal opportunity employer. NR 57 TC 1 Z9 1 U1 2 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD JUN PY 2015 VL 143 IS 3 BP 385 EP 392 DI 10.1007/s10709-015-9837-2 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA CH5FK UT WOS:000354060500013 PM 25896369 ER PT J AU Pederson, N D'Amato, AW Dyer, JM Foster, DR Goldblum, D Hart, JL Hessl, AE Iverson, LR Jackson, ST Martin-Benito, D McCarthy, BC McEwan, RW Mladenoff, DJ Parker, AJ Shuman, B Williams, JW AF Pederson, Neil D'Amato, Anthony W. Dyer, James M. Foster, David R. Goldblum, David Hart, Justin L. Hessl, Amy E. Iverson, Louis R. Jackson, Stephen T. Martin-Benito, Dario McCarthy, Brian C. McEwan, Ryan W. Mladenoff, David J. Parker, Albert J. Shuman, Bryan Williams, John W. TI Climate remains an important driver of post-European vegetation change in the eastern United States SO GLOBAL CHANGE BIOLOGY LA English DT Letter ID NORTHERN RED OAK; WHITE OAK; RADIAL GROWTH; HARDWOOD FOREST; TREE MORTALITY; HABITAT MODELS; QUERCUS-ALBA; DROUGHT; VARIABILITY; RESPONSES C1 [Pederson, Neil] Lamont Doherty Earth Observ, Tree Ring Lab, Palisades, NY 10964 USA. [D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Dyer, James M.] Ohio Univ, Dept Geog, Athens, OH 45701 USA. [Foster, David R.] Harvard Univ, Harvard Forest, Petersham, MA 01366 USA. [Goldblum, David] No Illinois Univ, Dept Geog, De Kalb, IL 60115 USA. [Hart, Justin L.] Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. [Hessl, Amy E.] W Virginia Univ, Dept Geol & Geog, Morgantown, WV 26506 USA. [Iverson, Louis R.] US Forest Serv, Delaware, OH 43015 USA. [Jackson, Stephen T.] US Geol Survey, DOI Southwest Climate Sci Ctr, Tucson, AZ 85719 USA. [Jackson, Stephen T.] Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Martin-Benito, Dario] ETH, CHN G 63, Inst Terr Ecosyst, CH-8092 Zurich, Switzerland. [McCarthy, Brian C.] Ohio Univ, Dept Plant Biol, Athens, OH 45701 USA. [McEwan, Ryan W.] Univ Dayton, Dept Biol, Dayton, OH 45469 USA. [Mladenoff, David J.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Parker, Albert J.] Univ Georgia, Dept Geog, Athens, GA 30602 USA. [Shuman, Bryan] Univ Wyoming, Dept Geol & Geophys, Laramie, WY 82071 USA. [Williams, John W.] Univ Wisconsin, Dept Geog, Madison, WI 53706 USA. RP Pederson, N (reprint author), Harvard Univ, Harvard Forest, 324 North Main St, Petersham, MA 01366 USA. RI Hart, Justin/B-2535-2010; OI Hart, Justin/0000-0001-6580-0189; Martin-Benito, Dario/0000-0002-6738-3312 NR 64 TC 12 Z9 12 U1 7 U2 65 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2015 VL 21 IS 6 BP 2105 EP 2110 DI 10.1111/gcb.12779 PG 6 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CH4BZ UT WOS:000353977500001 PM 25477234 ER PT J AU Cavaleri, MA Reed, SC Smith, WK Wood, TE AF Cavaleri, Molly A. Reed, Sasha C. Smith, W. Kolby Wood, Tana E. TI Urgent need for warming experiments in tropical forests SO GLOBAL CHANGE BIOLOGY LA English DT Editorial Material DE carbon flux; CMIP5; ecosystem processes; global warming; net ecosystem production; temperature; temperature threshold; tipping point; tropics; warming manipulation ID CLIMATE-CHANGE EXPERIMENTS; INFRARED HEATER ARRAYS; GLOBAL CHANGE RESEARCH; DRY-SEASON IRRIGATION; AMAZONIAN RAIN-FOREST; CARBON-DIOXIDE UPTAKE; TERRESTRIAL ECOSYSTEMS; TEMPERATURE SENSITIVITY; EXPERIMENTAL DROUGHT; SOIL RESPIRATION AB Although tropical forests account for only a fraction of the planet's terrestrial surface, they exchange more carbon dioxide with the atmosphere than any other biome on Earth, and thus play a disproportionate role in the global climate. In the next 20 years, the tropics will experience unprecedented warming, yet there is exceedingly high uncertainty about their potential responses to this imminent climatic change. Here, we prioritize research approaches given both funding and logistical constraints in order to resolve major uncertainties about how tropical forests function and also to improve predictive capacity of earth system models. We investigate overall model uncertainty of tropical latitudes and explore the scientific benefits and inevitable trade-offs inherent in large-scale manipulative field experiments. With a Coupled Model Intercomparison Project Phase 5 analysis, we found that model variability in projected net ecosystem production was nearly 3 times greater in the tropics than for any other latitude. Through a review of the most current literature, we concluded that manipulative warming experiments are vital to accurately predict future tropical forest carbon balance, and we further recommend the establishment of a network of comparable studies spanning gradients of precipitation, edaphic qualities, plant types, and/or land use change. We provide arguments for long-term, single-factor warming experiments that incorporate warming of the most biogeochemically active ecosystem components (i.e. leaves, roots, soil microbes). Hypothesis testing of underlying mechanisms should be a priority, along with improving model parameterization and constraints. No single tropical forest is representative of all tropical forests; therefore logistical feasibility should be the most important consideration for locating large-scale manipulative experiments. Above all, we advocate for multi-faceted research programs, and we offer arguments for what we consider the most powerful and urgent way forward in order to improve our understanding of tropical forest responses to climate change. C1 [Cavaleri, Molly A.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Reed, Sasha C.] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. [Smith, W. Kolby] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. [Wood, Tana E.] US Forest Serv, USDA, Int Inst Trop Forestry, Jardin Bot Sur, Rio Piedras, PR 00926 USA. [Wood, Tana E.] Fdn Puertorriquena Conservac, San Juan, PR 00936 USA. RP Cavaleri, MA (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. EM macavale@mtu.edu OI Smith, William/0000-0002-5785-6489 NR 126 TC 23 Z9 24 U1 16 U2 96 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2015 VL 21 IS 6 BP 2111 EP 2121 DI 10.1111/gcb.12860 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CH4BZ UT WOS:000353977500002 PM 25641092 ER PT J AU Rich, RL Stefanski, A Montgomery, RA Hobbie, SE Kimball, BA Reich, PB AF Rich, Roy L. Stefanski, Artur Montgomery, Rebecca A. Hobbie, Sarah E. Kimball, Bruce A. Reich, Peter B. TI Design and performance of combined infrared canopy and belowground warming in the B4WarmED (Boreal Forest Warming at an Ecotone in Danger) experiment SO GLOBAL CHANGE BIOLOGY LA English DT Article DE canopy temperature; climate change; ecosystem processes; global warming; infrared warming; soil temperature; soil warming; species composition; tree function; trees ID TRACE GAS FLUXES; HEATER ARRAYS; SPRING WHEAT; FIELD PLOTS; SOIL; ECOSYSTEM; TEMPERATURE; MICROCLIMATE; GROWTH; CO2 AB Conducting manipulative climate change experiments in complex vegetation is challenging, given considerable temporal and spatial heterogeneity. One specific challenge involves warming of both plants and soils to depth. We describe the design and performance of an open-air warming experiment called Boreal Forest Warming at an Ecotone in Danger (B4WarmED) that addresses the potential for projected climate warming to alter tree function, species composition, and ecosystem processes at the boreal-temperate ecotone. The experiment includes two forested sites in northern Minnesota, USA, with plots in both open (recently clear-cut) and closed canopy habitats, where seedlings of 11 tree species were planted into native ground vegetation. Treatments include three target levels of plant canopy and soil warming (ambient, +1.7 degrees C, +3.4 degrees C). Warming was achieved by independent feedback control of voltage input to aboveground infrared heaters and belowground buried resistance heating cables in each of 72-7.0m(2) plots. The treatments emulated patterns of observed diurnal, seasonal, and annual temperatures but with superimposed warming. For the 2009 to 2011 field seasons, we achieved temperature elevations near our targets with growing season overall mean differences (T-below) of +1.84 degrees C and +3.66 degrees C at 10cm soil depth and (T-above) of +1.82 degrees C and +3.45 degrees C for the plant canopies. We also achieved measured soil warming to at least 1m depth. Aboveground treatment stability and control were better during nighttime than daytime and in closed vs. open canopy sites in part due to calmer conditions. Heating efficacy in open canopy areas was reduced with increasing canopy complexity and size. Results of this study suggest the warming approach is scalable: it should work well in small-statured vegetation such as grasslands, desert, agricultural crops, and tree saplings (<5m tall). C1 [Rich, Roy L.; Stefanski, Artur; Montgomery, Rebecca A.; Reich, Peter B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Hobbie, Sarah E.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Kimball, Bruce A.] ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2753, Australia. RP Rich, RL (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM rich0475@umn.edu OI Hobbie, Sarah/0000-0001-5159-031X FU U.S. Department of Energy Program on Ecological Research 385 Grant [DE-FG02-07ER64456]; University of Minnesota: College of Food, Agricultural, and Natural Resources Sciences; Wilderness Research Foundation; Minnesota Department of Natural Resources FX Gratitude to the myriad and multitude of assistants, students, and colleagues who make large projects work and special thanks to Karen Rice, Kyle Gill, Kirk Wythers, Jacek Oleksyn, John Blanchard, Ron Severs, and Nate Russart. Funding provided by: U.S. Department of Energy Program on Ecological Research 385 Grant No. DE-FG02-07ER64456; University of Minnesota: College of Food, Agricultural, and Natural Resources Sciences; Wilderness Research Foundation; and Minnesota Department of Natural Resources. NR 45 TC 11 Z9 12 U1 13 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2015 VL 21 IS 6 BP 2334 EP 2348 DI 10.1111/gcb.12855 PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CH4BZ UT WOS:000353977500020 PM 25640748 ER PT J AU Ogut, F Bian, Y Bradbury, PJ Holland, JB AF Ogut, F. Bian, Y. Bradbury, P. J. Holland, J. B. TI Joint-multiple family linkage analysis predicts within-family variation better than single-family analysis of the maize nested association mapping population SO HEREDITY LA English DT Article ID QUANTITATIVE TRAIT LOCI; MARKER-ASSISTED SELECTION; GENOME-WIDE ASSOCIATION; SOUTHERN LEAF-BLIGHT; GENETIC ARCHITECTURE; FLOWERING-TIME; COMPLEX TRAITS; POSTDOMESTICATION SPREAD; PHOTOPERIOD SENSITIVITY; CONNECTED POPULATIONS AB Quantitative trait locus (QTL) mapping has been used to dissect the genetic architecture of complex traits and predict phenotypes for marker-assisted selection. Many QTL mapping studies in plants have been limited to one biparental family population. Joint analysis of multiple biparental families offers an alternative approach to QTL mapping with a wider scope of inference. Joint-multiple population analysis should have higher power to detect QTL shared among multiple families, but may have lower power to detect rare QTL. We compared prediction ability of single-family and joint-family QTL analysis methods with fivefold cross-validation for 6 diverse traits using the maize nested association mapping population, which comprises 25 biparental recombinant inbred families. Joint-family QTL analysis had higher mean prediction abilities than single-family QTL analysis for all traits at most significance thresholds, and was always better at more stringent significance thresholds. Most robust QTL (detected in 450% of data samples) were restricted to one family and were often not detected at high frequency by joint-family analysis, implying substantial genetic heterogeneity among families for complex traits in maize. The superior predictive ability of joint-family QTL models despite important genetic differences among families suggests that joint-family models capture sufficient smaller effect QTL that are shared across families to compensate for missing some rare large-effect QTL. C1 [Ogut, F.; Bian, Y.; Holland, J. B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Bradbury, P. J.] ARS, USDA, Plant Soil & Nutr Res Unit, Ithaca, NY USA. [Holland, J. B.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC USA. RP Holland, JB (reprint author), N Carolina State Univ, Dept Crop Sci, Campus Box 7620, Raleigh, NC 27695 USA. EM james_holland@ncsu.edu OI Holland, James/0000-0002-4341-9675 FU US Department of Agriculture, Agricultural Research Service; National Science Foundation [IOS-1238014, IOS-0820619] FX Research supported by US Department of Agriculture, Agricultural Research Service and the National Science Foundation grants IOS-1238014 and IOS-0820619. NR 40 TC 7 Z9 7 U1 1 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD JUN PY 2015 VL 114 IS 6 BP 552 EP 563 DI 10.1038/hdy.2014.123 PG 12 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA CH9WI UT WOS:000354386700004 PM 25585918 ER PT J AU Chapman, NC Beekman, M Allsopp, MH Rinderer, TE Lim, J Oxley, PR Oldroyd, BP AF Chapman, N. C. Beekman, M. Allsopp, M. H. Rinderer, T. E. Lim, J. Oxley, P. R. Oldroyd, B. P. TI Inheritance of thelytoky in the honey bee Apis mellifera capensis SO HEREDITY LA English DT Article ID REPRODUCTIVE DOMINANCE; POPULATION-STRUCTURE; WORKER REPRODUCTION; PARTHENOGENESIS; SCUTELLATA; AFRICA; TRAITS; DNA; L.; MICROSATELLITE AB Asexual reproduction via thelytokous parthenogenesis is widespread in the Hymenoptera, but its genetic underpinnings have been described only twice. In the wasp Lysiphlebus fabarum and the Cape honey bee Apis mellifera capensis the origin of thelytoky have each been traced to a single recessive locus. In the Cape honey bee it has been argued that thelytoky (th) controls the thelytoky phenotype and that a deletion of 9 bp in the flanking intron downstream of exon 5 (tae) of the gemini gene switches parthenogenesis from arrhenotoky to thelytoky. To further explore the mode of inheritance of thelytoky, we generated reciprocal backcrosses between thelytokous A. m. capensis and the arrhenotokous A. m. scutellata. Ten genetic markers were used to identify 108 thelytokously produced offspring and 225 arrhenotokously produced offspring from 14 colonies. Patterns of appearance of thelytokous parthenogenesis were inconsistent with a single locus, either th or tae, controlling thelytoky. We further show that the 9 bp deletion is present in the arrhenotokous A. m. scutellata population in South Africa, in A. m. intermissa in Morocco and in Africanized bees from Brazil and Texas, USA, where thelytoky has not been reported. Thus the 9 bp deletion cannot be the cause of thelytoky. Further, we found two novel tae alleles. One contains the previously described 9 bp deletion and an additional deletion of 7 bp nearby. The second carries a single base insertion with respect to the wild type. Our data are consistent with the putative th locus increasing reproductive capacity. C1 [Chapman, N. C.; Beekman, M.; Lim, J.; Oxley, P. R.; Oldroyd, B. P.] Univ Sydney, Behav & Genet Social Insects Lab, Sch Biol Sci A12, Sydney, NSW 2006, Australia. [Allsopp, M. H.] ARC Plant Protect Res Inst, Stellenbosch, South Africa. [Rinderer, T. E.] USDA ARS, Honey Bee Breeding Genet & Physiol Res Lab, Baton Rouge, LA 70820 USA. RP Chapman, NC (reprint author), Univ Sydney, Behav & Genet Social Insects Lab, Sch Biol Sci, Macleay Bldg A12, Sydney, NSW 2006, Australia. EM nadine.chapman@sydney.edu.au RI Beekman, Madeleine/A-3983-2011 FU Australian Research Council; honey bee program of the Rural Industries Research and Development Corporation FX BPO and MB are supported by the Australian Research Council. NC and BPO are supported by the honey bee program of the Rural Industries Research and Development Corporation. We thank members of the Behaviour and Genetics of Social Insects Lab and four anonymous reviewers for constructive criticism that improved the manuscript. NR 63 TC 5 Z9 5 U1 6 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD JUN PY 2015 VL 114 IS 6 BP 584 EP 592 DI 10.1038/hdy.2014.127 PG 9 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA CH9WI UT WOS:000354386700007 PM 25585920 ER PT J AU Meissen, JK Hirahatake, KM Adams, SH Fiehn, O AF Meissen, John K. Hirahatake, Kristin M. Adams, Sean H. Fiehn, Oliver TI Temporal metabolomic responses of cultured HepG2 liver cells to high fructose and high glucose exposures SO METABOLOMICS LA English DT Article DE High fructose corn syrup; Metabolic networks; Time-of-flight mass spectrometry; Chromatography; Lipidomics ID FATTY-ACID OXIDATION; INSULIN SENSITIVITY; CARBOHYDRATE-METABOLISM; LIPOGENIC ENZYMES; DIETARY FRUCTOSE; RATS; NETWORKS; PHOSPHATIDYLETHANOLAMINE; DEHYDROGENASE; CONSUMPTION AB High fructose consumption has been implicated with deleterious effects on human health, including hyperlipidemia elicited through de novo lipogenesis. However, more global effects of fructose on cellular metabolism have not been elucidated. In order to explore the metabolic impact of fructose-containing nutrients, we applied both GC-TOF and HILIC-QTOF mass spectrometry metabolomic strategies using extracts from cultured HepG2 cells exposed to fructose, glucose, or fructose + glucose. Cellular responses were analyzed in a time-dependent manner, incubated in media containing 5.5 mM glucose + 5.0 mM fructose in comparison to controls incubated in media containing either 5.5 mM glucose or 10.5 mM glucose. Mass spectrometry identified 156 unique known metabolites and a large number of unknown compounds, which revealed metabolite changes due to both utilization of fructose and high-carbohydrate loads independent of hexose structure. Fructose was shown to be partially converted to sorbitol, and generated higher levels of fructose-1-phosphate as a precursor for glycolytic intermediates. Differentially regulated ratios of 3-phosphoglycerate to serine pathway intermediates in high fructose media indicated a diversion of carbon backbones away from energy metabolism. Additionally, high fructose conditions changed levels of complex lipids toward phosphatidylethanolamines. Patterns of acylcarnitines in response to high hexose exposure (10.5 mM glucose or glucose/fructose combination) suggested a reduction in mitochondrial beta-oxidation. C1 [Meissen, John K.; Fiehn, Oliver] Univ Calif Davis, UC Davis West Coast Metabol Ctr, Davis, CA 95616 USA. [Hirahatake, Kristin M.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Hirahatake, Kristin M.; Adams, Sean H.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Fiehn, Oliver] King Abdulaziz Univ, Dept Biochem, Fac Sci, Jeddah 21589, Saudi Arabia. RP Fiehn, O (reprint author), Univ Calif Davis, UC Davis West Coast Metabol Ctr, 451 Hlth Sci Dr, Davis, CA 95616 USA. EM ofiehn@ucdavis.edu FU National Institutes of Health [U24 DK097154, R01 DK078328, P20 HL113452]; Diabetes Action and Research Education Fund [U24 DK097154]; USDA-ARS [51530-016-00D, 5306-51530-019-00] FX We thank Wan Tan and Gert Wohlgemuth for their assistance with the GC-TOF platform. Financial support was provided by the National Institutes of Health U24 DK097154 (O.F.), R01 DK078328 (S.H.A., O.F.) and P20 HL113452 (O.F.), the Diabetes Action and Research Education Fund U24 DK097154 (S.H.A), and USDA-ARS Intramural Projects 51530-016-00D and 5306-51530-019-00 (S.H.A.). NR 57 TC 1 Z9 1 U1 2 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD JUN PY 2015 VL 11 IS 3 BP 707 EP 721 DI 10.1007/s11306-014-0729-8 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CH6HL UT WOS:000354137100016 PM 26190955 ER PT J AU Yocum, GD Rinehart, JP Horvath, DP Kemp, WP Bosch, J Alroobi, R Salem, S AF Yocum, George D. Rinehart, Joseph P. Horvath, David P. Kemp, William P. Bosch, Jordi Alroobi, Rami Salem, Saeed TI Key molecular processes of the diapause to post-diapause quiescence transition in the alfalfa leafcutting bee Megachile rotundata identified by comparative transcriptome analysis SO PHYSIOLOGICAL ENTOMOLOGY LA English DT Article DE Development; diapause; gene expression; KEGG pathways; pollinator ID FLY SARCOPHAGA-CRASSIPALPIS; MOSQUITO CULEX-PIPIENS; ASIAN TIGER MOSQUITO; CELL-CYCLE ARREST; FLESH FLY; GENE-EXPRESSION; DROSOPHILA-MELANOGASTER; PUPAL DIAPAUSE; MANDUCA-SEXTA; SOLITARY BEE AB Insect diapause (dormancy) synchronizes an insect's life cycle to seasonal changes in the abiotic and biotic resources required for development and reproduction. Transcription analysis of diapause to post-diapause quiescent transition in the alfalfa leafcutting bee Megachile rotundataFabricius identifies 643 post-diapause up-regulated gene transcripts and 242 post-diapause down-regulated transcripts. The log(2) fold change in gene expression levels ranges from -5 to 7. Transcripts from several pivotal diapause-related processes, including chromatin remodelling, cellular signalling pathways, microRNA processing, anaerobic glycolysis, cell cycle arrest and neuroendocrine control, are identified as being differentially expressed during the diapause to post-diapause transition. In conjunction with studies from other insect species, the data indicate that there are several common mechanisms of diapause control and maintenance. C1 [Yocum, George D.; Rinehart, Joseph P.; Horvath, David P.; Kemp, William P.] ARS, Biosci Res Lab, USDA, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. [Bosch, Jordi] Univ Autonoma Barcelona, CREAF, Bellaterra, Spain. [Alroobi, Rami; Salem, Saeed] N Dakota State Univ, Dept Comp Sci, Fargo, ND 58105 USA. RP Yocum, GD (reprint author), ARS, Biosci Res Lab, USDA, Red River Valley Agr Res Ctr, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM george.yocum@ars.usda.gov OI Bosch , Jordi/0000-0002-8088-9457 NR 79 TC 4 Z9 4 U1 3 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6962 EI 1365-3032 J9 PHYSIOL ENTOMOL JI Physiol. Entomol. PD JUN PY 2015 VL 40 IS 2 BP 103 EP 112 DI 10.1111/phen.12093 PG 10 WC Entomology SC Entomology GA CH6FM UT WOS:000354131800001 ER PT J AU Vinterhalter, B Krstic-Milosevic, D Jankovic, T Pljevljakusic, D Ninkovic, S Smigocki, A Vinterhalter, D AF Vinterhalter, Branka Krstic-Milosevic, Dijana Jankovic, Teodora Pljevljakusic, Dejan Ninkovic, Slavica Smigocki, Ann Vinterhalter, Dragan TI Gentiana dinarica Beck. hairy root cultures and evaluation of factors affecting growth and xanthone production SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Gentiana dinarica; Agrobacterium rhizogenes; Carbohydrates; Xanthone; Total phenolics; DPPH ID RHIZOGENES-MEDIATED TRANSFORMATION; AGROBACTERIUM-RHIZOGENES; GENETIC-TRANSFORMATION; SWERTIA-MUSSOTII; IN-VITRO; HIGHER-PLANTS; GENTIOPICROSIDE; ESTABLISHMENT; SECOIRIDOIDS; REGENERATION AB The induction and establishment of hairy root cultures of Gentiana dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and 15834/PI) is reported for the first time. Strain 15834/PI had higher induction rate of hairy roots (32.15 %) than strain A4M70GUS (6.12 %). Transgenic nature of the roots was confirmed by GUS assay and PCR analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and -3) with marked differences in general morphology and growth rate were further studied. The methanol extracts of hairy root clones were analyzed for xanthones content using HPLC method. The effects of the type of carbohydrate source (sucrose, fructose or glucose) at different concentrations on the growth parameters (growth index, dry weight, fresh/dry weight ratio), phenolic and xanthone production, and free radical scavenging activity of the transgenic clones were evaluated. Statistical two level factorial design was used to define optimal conditions for growth and successful secondary metabolite production in G. dinarica hairy root clones. The results showed that clones A4M70GUS-D and 15834/PI-3 were the superior ones. These two clones had the highest dry weight on 116.8 mM sucrose, producing up to threefold higher amounts of total phenolics and norswertianin-1-O-primeveroside than other clones, untransformed roots and roots of wild-grown plants. C1 [Vinterhalter, Branka; Krstic-Milosevic, Dijana; Ninkovic, Slavica; Vinterhalter, Dragan] Univ Belgrade, Inst Biol Res Sinisa Stankovic, Belgrade 11060, Serbia. [Jankovic, Teodora; Pljevljakusic, Dejan] Inst Med Plant Res Dr Josif Pancic, Belgrade 11000, Serbia. [Smigocki, Ann] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Jankovic, T (reprint author), Inst Med Plant Res Dr Josif Pancic, Tadeusa Koscuska 1, Belgrade 11000, Serbia. EM tjankovic@mocbilja.rs OI Krstic-Milosevic, Dijana/0000-0001-6820-4809; Pljevljakusic, Dejan/0000-0003-2362-8290 FU Ministry of Education, Science and Technological Development of the Republic of Serbia [173015] FX Bacterial strain A4M70GUS was obtained by courtesy of Dr. Landre, Univ. Pierre and Marie Curie, Paris, France. The present work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Grant No. 173015. NR 51 TC 2 Z9 2 U1 0 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD JUN PY 2015 VL 121 IS 3 BP 667 EP 679 DI 10.1007/s11240-015-0737-z PG 13 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CH5TR UT WOS:000354098700013 ER PT J AU Zhang, JX Yuan, H Fei, ZJ Pogson, BJ Zhang, LG Li, L AF Zhang, Junxiang Yuan, Hui Fei, Zhangjun Pogson, Barry J. Zhang, Lugang Li, Li TI Molecular characterization and transcriptome analysis of orange head Chinese cabbage (Brassica rapa L. ssp pekinensis) SO PLANTA LA English DT Article DE Chinese cabbage; Orange head; BrCRTISO; Transcriptome; Gene-specific marker ID BETA-CAROTENE ACCUMULATION; LEAF DEVELOPMENT; ARABIDOPSIS; GENE; PLANTS; METABOLISM; EXPRESSION; MARKERS; IDENTIFICATION; BIOSYNTHESIS AB The orange head phenotype of Br - or resulted from a large insertion in carotenoid isomerase (BrCRTISO) . Comparative transcriptome analysis revealed that the mutation affected the expression of abundant transcription factor genes. A new orange trait-specific marker was developed for marker-assisted breeding. Orange head leaves are a desirable quality trait for Chinese cabbage. Our previous fine mapping identified BrCRTISO as the Br-or candidate gene for the orange Chinese cabbage mutant. Here, we examined the BrCRTISO gene from white and orange head Chinese cabbage. While BrCRTISO from the white control plant was able to complement the Arabidopsis Atcrtiso mutant phenotype, Brcrtiso with a large insertion from the orange head Chinese cabbage failed to rescue the Arabidopsis mutant phenotype. The results show that Brcrtiso was non-functional, concomitant with the accumulation of prolycopene in Br-or to yield orange head. Comparative transcriptome analysis by RNA-seq identified 372 differentially expressed genes between the control and Br-or mutant using two near-isogenic lines with white and orange inner leaves. The mutation in BrCRTISO specifically affected many genes in the functional groups involved in RNA, protein, transport, and signaling. Particularly, expressions of many transcription factor genes were dramatically altered in Br-or, suggesting a potential role of BrCRTISO or carotenoid metabolites in affecting transcription. A novel co-dominant gene-specific marker was developed that co-segregated with orange color phenotype and would be useful for marker-assisted selection with enhanced selection efficiency. Our study provides new insights into understanding of the molecular basis of Br-or in mediating head leaf color and depicts a global view of the effect of BrCRTISO on cellular processes in plant. It also provides a molecular tool to accelerate breeding new Chinese cabbage cultivars with unique health quality and visual appearance. C1 [Zhang, Junxiang; Zhang, Lugang] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Area, Yangling 712100, Peoples R China. [Zhang, Junxiang; Yuan, Hui; Li, Li] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Pogson, Barry J.] Australian Natl Univ, Australian Res Council, Ctr Excellence Plant Energy Biol, Canberra, ACT 0200, Australia. [Li, Li] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. RP Zhang, LG (reprint author), Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Area, Yangling 712100, Peoples R China. EM lugangzh@163.com; ll37@cornell.edu RI Pogson, Barry/C-9953-2009; Yuan, Hui/H-6723-2016 OI Pogson, Barry/0000-0003-1869-2423; FU China Scholarship Council (CSC); USDA-ARS; National Natural Science Foundation of China [31171965]; 863 plan [2012AA100105]; Science and technology support program of China [2012BAD02B01] FX We thank Drs. Xiangjun Zhou, Mingke Zhang, and Huamin Zhang for their help and technical advice, Dr. Jiping Liu for kindly providing the pCAMBIA1305.1 vector. JZ thanks the China Scholarship Council (CSC) for support. This work was partially supported by USDA-ARS-based fund and by the National Natural Science Foundation of China (No. 31171965), 863 plan (2012AA100105), and Science and technology support program of China (2012BAD02B01). NR 53 TC 3 Z9 3 U1 4 U2 49 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD JUN PY 2015 VL 241 IS 6 BP 1381 EP 1394 DI 10.1007/s00425-015-2262-z PG 14 WC Plant Sciences SC Plant Sciences GA CH7VM UT WOS:000354244700007 PM 25686795 ER PT J AU Zhang, YL Yao, SH Mao, JD Olk, DC Cao, XY Zhang, B AF Zhang, Yueling Yao, Shuihong Mao, Jingdong Olk, Daniel C. Cao, Xiaoyan Zhang, Bin TI Chemical composition of organic matter in a deep soil changed with a positive priming effect due to glucose addition as investigated by C-13 NMR spectroscopy SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE C-13 labeling; Priming effect; Deep soil; Soil organic carbon; C-13-CP/TOSS NMR; Dipolar dephasing ID SOLID-STATE NMR; NUCLEAR-MAGNETIC-RESONANCE; FOREST SOIL; MICROBIAL COMMUNITY; VERTICAL-DISTRIBUTION; HUMIC SUBSTANCES; SIZE FRACTIONS; CARBON INPUT; ACID; DECOMPOSITION AB Fresh organic carbon becomes more accessible to deep soil following losses of surface soil and deep intentional incorporation of crop residues, which can cause the priming effect and influence the quality and quantity of SOC in deep soil. This study determined the priming effect due to addition of water-dissolved C-13-labeled glucose (0.4 g C kg(-1) soil) to a soil taken from 1.00 to 1.20 m depth. The changes in chemical compositions of SOC in soils without (G(0)) and with (G(0.4)) glucose addition during a 31-d incubation were investigated with solid-state C-13 cross polarization/total sideband suppression (C-13-CP/TOSS) and CP/TOSS with dipolar dephasing nuclear magnetic resonance (NMR) techniques. No glucose remained in the soil after 21 days of incubation, with 48% being completely mineralized into CO2 emission and 52% being incorporated into SOC. The native SOC was decomposed by 0.23% more in G(0.4) than in Go. The NMR spectra demonstrated that both labile and recalcitrant organic compounds in SOC changed during the incubation, but in different manners in G(0) and G(0.4). During the incubation, the -(CH2)(n)-abundance in Go did not change over time, but in G(0.4) it decreased from Day 0 to Day 21 and then increased from Day 21 to Day 31, suggesting shifts of soil microbial communities only in G(0.4). After the incubation, in Go the abundances of ketones/aldehydes and nonpolar alkyl C increased, but those of aromatic C C and protonated O-alkyl C (OCH) decreased; In G(0.4), the abundances of NCH and protonated O-alkyl C (OCH) increased, but those of nonpolar alkyl C and nonprotonated aromatic C-O and ketones/aldehydes decreased. Such inconsistent changes in recalcitrant compounds between Go and G(0.4) indicated that glucose addition likely primed the decomposition of aromatic C-O and suppressed the formation of ketones/aldehydes. We have demonstrated for the first time that the priming effect of SOC decomposition in the deep soil was involved with larger notable changes in both labile and recalcitrant structures of native SOC due to glucose addition compared with that without glucose addition. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Zhang, Yueling; Yao, Shuihong; Zhang, Bin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. [Zhang, Yueling] China Agr Univ, Coll Resources & Environm Sci, Beijing 100083, Peoples R China. [Zhang, Bin] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Natl Field Observat & Res Stn Hailun Agroecosyst, Key Lab Mollisols Agroecol, Harbin 150081, Peoples R China. [Mao, Jingdong; Cao, Xiaoyan] Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. [Olk, Daniel C.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Zhang, B (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. EM zhangbin01@caas.cn RI Cao, Xiaoyan/E-3492-2012 OI Cao, Xiaoyan/0000-0001-7571-6482 FU National Natural Science Foundation of China [41330855]; seed fund program of Chinese Academy of Agricultural Sciences (CAAS [2011-06]; Hundred Talents Program of the Chinese Academy of Sciences FX This study was funded by the National Natural Science Foundation of China (41330855) and the seed fund program of Chinese Academy of Agricultural Sciences (CAAS, 2011-06). We thank Tusheng Ren for his suggestions to the experiment. Bin Zhang was supported by the Hundred Talents Program of the Chinese Academy of Sciences. NR 69 TC 3 Z9 4 U1 16 U2 92 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2015 VL 85 BP 137 EP 144 DI 10.1016/j.soilbio.2015.03.013 PG 8 WC Soil Science SC Agriculture GA CH9GV UT WOS:000354344900017 ER PT J AU Bell, CW Asao, S Calderon, F Wolk, B Wallenstein, MD AF Bell, Colin W. Asao, Shinichi Calderon, Francisco Wolk, Brett Wallenstein, Matthew D. TI Plant nitrogen uptake drives rhizosphere bacterial community assembly during plant growth SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Rhizosphere bacterial assembly; Plant nitrogen uptake; Nutrient stoichiometry; Microbial biomass; Bromus tectorum; C3 native grasses ID SOIL MICROBIAL COMMUNITIES; CHIHUAHUAN DESERT GRASSLAND; N-P STOICHIOMETRY; TERRESTRIAL ECOSYSTEMS; ENZYME-ACTIVITIES; ORGANIC-MATTER; BIOMASS-C; DIVERSITY; CARBON; FEEDBACKS AB When plants establish in novel environments, they can modify soil microbial community structure and functional properties in ways that enhance their own success. Although soil microbial communities are influenced by abiotic environmental variability, rhizosphere microbial communities may also be affected by plant activities such as nutrient uptake during the growing season. We predicted that during the growing season, plant N uptake would explain much of the variation in rhizosphere microbial community assembly and functional traits. We grew the invasive C-3 grass Bromus tectorum and three commonly co-occurring native C-3 grasses in a controlled greenhouse environment, and examined rhizosphere bacterial community structural and functional characteristics at three different plant growth stages. We found that soil N availability and plant tissue N levels strongly correlated with shifts in rhizosphere bacterial community structure. It also appeared that the rapid drawdown of soil nutrients in the rhizosphere during the plant growing season triggered a selection event whereby only those microbes able to tolerate the changing nutrient conditions were able to persist. Plant N uptake rates inversely corresponded to microbial biomass N levels during periods of peak plant growth. Mechanisms which enable plants to influence rhizosphere bacterial community structure and function are likely to affect their competitive ability and fitness. Our study suggests that plants can alter their rhizosphere microbiomes through influencing nutrient availability. The ways in which plants establish their rhizosphere bacterial communities may now be viewed as a selection trait related to intrinsic plant species nutrient demands. Published by Elsevier Ltd. Published by Elsevier Ltd. C1 [Bell, Colin W.; Asao, Shinichi; Wallenstein, Matthew D.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80524 USA. [Calderon, Francisco] USDA ARS, Cent Great Plains Resources Management Res, Akron, CO 80720 USA. [Wolk, Brett] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Wallenstein, Matthew D.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. RP Bell, CW (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80524 USA. EM colin.bell@colostate.edu RI Wallenstein, Matthew/C-6441-2008; Asao, Shinichi/R-9514-2016 OI Wallenstein, Matthew/0000-0002-6219-1442; Asao, Shinichi/0000-0002-0334-5464 FU Warner College of Natural Resources; National Science Foundation [1020540] FX This research was funded by a Warner College of Natural Resources (CSU) mini grant awarded to C.B. and award 1020540 to M.W. from the National Science Foundation. We would like to thank the Mark Paschke and his Restoration Ecology Lab members for help with the extensive greenhouse setup and operations required to accomplish this research effort. NR 100 TC 4 Z9 6 U1 7 U2 134 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD JUN PY 2015 VL 85 BP 170 EP 182 DI 10.1016/j.soilbio.2015.03.006 PG 13 WC Soil Science SC Agriculture GA CH9GV UT WOS:000354344900021 ER PT J AU Bakst, MR Bauchan, G AF Bakst, Murray R. Bauchan, Gary TI Apical blebs on sperm storage tubule epithelial cell microvilli: Their release and interaction with resident sperm in the turkey hen oviduct SO THERIOGENOLOGY LA English DT Article DE Bird; Poultry; Oviductal sperm storage; Sperm storage tubule ID GALLUS-DOMESTICUS; SPERMATOZOA; GLANDS; EPIDIDYMOSOMES; MATURATION; PHYSIOLOGY; MECHANISM; VESICLES; MODEL AB Located at the anterior end of the turkey hen's vagina are numerous discrete tubular invaginations of the surface epithelium, collectively referred to as the sperm storage tubules (SSTs). After mating or artificial insemination, sperm ascend the vagina, enter the SSTs, and over the ensuing days and weeks, gradually exit the SSTs and are transported to the anterior end of the oviduct to fertilize a daily succession of ova. Little is known regarding the cellular and molecular mechanisms responsible for sperm subsistence in the lumen of the SST. In this study, the origin of microvillus blebs (MvBs) on the apical tips of SST epithelial cells was examined, and their possible role in sperm survival was discussed. Regardless, if sperm are present or not, transmission electron microscopy revealed two types of microvilli differentiated by the presence or absence of pleomorphic unilaminar MvBs localized to their apical tips. Although some MvBs appeared to be discharging their contents into the SST lumen, others appeared to have pinched off the microvillus stem. When SSTs contained clusters of densely packed sperm, the sperm heads of those sperm adjacent to the SST epithelial cell surface were surrounded by the microvilli. Associated with the plasmalemma of sperm throughout the SST lumina were membrane fragments and small vesicles (30-130 nm in diameter), some of which appeared to have fused with sperm. It is concluded that the MvBs are a form of shedding vesicle released from the SST epithelial cell microvilli by apocrine secretion. On the basis of observations described herein and those of other authors, it is suggested that the MvBs contribute to sustained sperm storage in the SSTs by (1) supplying metabolic substrates used by resident sperm, (2) serving as fusogenic vehicles providing exogenous macromolecules that reversibly suppress sperm functions associated with fertilization (decapacitation?) and stabilize the sperm plasmalemma, and (3) acting as transport vesicles actively transporting fluid from the SST epithelial cells to the SST lumen. Published by Elsevier Inc. C1 [Bakst, Murray R.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Bauchan, Gary] ARS, Electron & Confocal Microscopy Unit, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. RP Bakst, MR (reprint author), ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM murray.bakst@ars.usda.gov NR 25 TC 9 Z9 9 U1 1 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD JUN PY 2015 VL 83 IS 9 BP 1438 EP 1444 DI 10.1016/j.theriogenology.2015.01.016 PG 7 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA CH3LE UT WOS:000353931400008 PM 25754776 ER PT J AU Ziska, LH Tomecek, MB Valerio, M Thompson, JP AF Ziska, L. H. Tomecek, M. B. Valerio, M. Thompson, J. P. TI Evidence for recent evolution in an invasive species, Microstegium vimineum, Japanese stiltgrass SO WEED RESEARCH LA English DT Article DE local adaptation; invasive species; nitrogen; light ID PHENOTYPIC PLASTICITY; ADAPTIVE EVOLUTION; RANGE EXPANSION; UNITED-STATES; C-4 GRASS; PLANT; ENVIRONMENTS; POPULATIONS; ADAPTATION; BREADTH AB The establishment and spread of invasive plants are often associated with a general-purpose genotype', with a corresponding high degree of phenotypic plasticity when introduced to a new environment. Evolutionary potential of invasive species following introduction should also be considered, however, as such changes can facilitate rapid range expansion. In this study, we utilised seed from three geographically distinct mid-Atlantic populations of an invasive species, Microstegium vimineum (Japanese stiltgrass), to assess whether populations varied in their biomass and reproductive responses to light and nitrogen under neutral glasshouse conditions. To assess evolutionary adaptation, we quantified and correlated the abiotic environment from each collection location with thefinal biomass and seed production of that population under common glasshouse conditions. For M.vimineum, growth and reproductive responses to a common set of abiotic parameters (e.g. light) under these conditions indicated considerable phenotypic variation between and within populations. Interestingly, the degree of variation of seed production and final biomass among populations in the glasshouse was strongly correlated with growing season length and cumulative degree days recorded near each population location. These latter data indicated that M.vimineum, introduced into this region <100years ago, may be undergoing adaptive evolution. Although additional populations of M.vimineum need to be examined, this study suggests that local evolutionary adaptation may be occurring rapidly and could, potentially, be an important aspect in the establishment and spread of this invasive species. C1 [Ziska, L. H.; Tomecek, M. B.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Valerio, M.] Univ Basilicata, Sch Agr Forest Food & Environm Sci, I-85100 Potenza, Italy. [Thompson, J. P.] United States Natl Invas Species Council Advisory, Washington, DC USA. RP Ziska, LH (reprint author), USDA ARS, Bldg 1,Room 323,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM l.ziska@ars.usda.gov NR 35 TC 2 Z9 2 U1 5 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD JUN PY 2015 VL 55 IS 3 BP 260 EP 267 DI 10.1111/wre.12138 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CH7XC UT WOS:000354248900005 ER PT J AU Watson, AM Barrows, FT Place, AR AF Watson, Aaron M. Barrows, Frederic T. Place, Allen R. TI Leaching of taurine from commercial type aquaculture feeds SO AQUACULTURE RESEARCH LA English DT Article DE taurine; leaching; feeds; amino acids ID FLOUNDER PARALICHTHYS-OLIVACEUS; CASEIN-BASED DIET; GROWTH-PERFORMANCE; RACHYCENTRON-CANADUM; ENHANCES GROWTH; AMINO-ACID; SUPPLEMENTATION; JUVENILE; PROTEIN; FISH AB Leaching of soluble compounds from pelleted feeds is an issue for the aquaculture industry through increased environmental impact and reduced ingestion essential components. This study was undertaken to examine the leaching rates of taurine, a non-protein amino acid with critical physiological roles in teleosts. To this end we adapted a new liquid chromatography mass spectrometry (LC-MS) method for quantifying taurine. Twelve different feeds (4mm dia.) varying in protein source and taurine levels were examined. Fishmeal content ranged from 0.0% to 45.5% with taurine supplementation ranging from 0.0% to 5.0%. Taurine was extracted and quantified from individual pellets in triplicate at six time points (0, 1, 5, 10, 20 and 40min). Leaching rates ranged from 0.026 +/- 0.005 to 0.826 +/- 0.121mgmin(-1) over 40min at 27 degrees C and were strongly correlated to initial taurine content of the feeds (for distilled water n=12, P<0.001, R-2=0.91 for artificial seawater, 25 ppt, n=4, P=0.020, R-2=0.96). Loss of taurine from feeds was 59.5 +/- 16.5% after 40min. This study shows that a significant amount of taurine is lost over time from uneaten feed and that taurine supplementation should exceed requirement levels for slow consumers or feed being delivered as multiple additions. C1 [Watson, Aaron M.; Place, Allen R.] Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21202 USA. [Barrows, Frederic T.] ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID USA. RP Watson, AM (reprint author), Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, 701 East Pratt St, Baltimore, MD 21202 USA. EM watsona@umces.edu FU NOAA-EPP FX AW was partially supported by the NOAA-EPP funded LMRCSC. The authors would like to thank Schillinger Genetics, Inc. for donating seven of the feeds used in this study and Dr. Michael Rust and Dr. Ron Johnson for comments on the development of the manuscript. This is a contribution #4819 from the University of Maryland Center for Environmental Science, and a contribution #13-112 from the Institute of Marine and Environmental Technology. NR 23 TC 3 Z9 3 U1 2 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD JUN PY 2015 VL 46 IS 6 BP 1510 EP 1517 DI 10.1111/are.12309 PG 8 WC Fisheries SC Fisheries GA CH3ZU UT WOS:000353970800022 ER PT J AU Tipping, PW Martin, MR Pratt, PD Rayamajhi, MB Gettys, LA AF Tipping, Philip W. Martin, Melissa R. Pratt, Paul D. Rayamajhi, M. B. Gettys, L. A. TI Resource regulation of an invasive tree by a classical biological control agent SO BIOLOGICAL CONTROL LA English DT Article DE Oxyops vitiosa; Melaleuca quinquenervia; Classical biological control; Resource Regulation Hypothesis; Plant compensation; Herbivory ID OXYOPS-VITIOSA COLEOPTERA; MELALEUCA-QUINQUENERVIA; FIELD COLONIZATION; AUSTRALIAN WEEVIL; LIFE-HISTORY; HERBIVORE; GROWTH; CURCULIONIDAE; INSECTS; QUALITY AB The invasive tree Melaleuca quinquenervia experienced substantial declines in growth and reproduction in response to chronic herbivory by the defoliating weevil Oxyops vitiosa. Plants subjected to unrestricted defoliation replaced leaves that were more suitable for feeding by the next generation, a process envisioned by the Resource Regulation Hypothesis which posits that attack by one generation increases the amount of the preferred host resources for the next, resulting in a positive feedback loop for the herbivore. The production of juvenile replacement leaves stimulated additional bouts of oviposition and feeding by O. vitiosa, which ultimately produced positive effects for the herbivore with negative consequences for the plant. The addition of water resources to the plant prolonged the positive feedback loop such that more than twice as many insects were produced on irrigated versus non-irrigated trees. In a more simple, reassembled food web on M. quinquenervia, the lack of biotic constraints like parasitoids may have prevented the earlier termination of the feedback loop and thus increased the impact of the biological control agent on the target. The overall effectiveness of this classical biological control program can be attributed, in part, to the phenomenon of the target plant's induced susceptible response to a herbivore. Published by Elsevier Inc. C1 [Tipping, Philip W.; Martin, Melissa R.; Pratt, Paul D.; Rayamajhi, M. B.] ARS, USDA, Invas Plant Res Lab, Davie, FL 33314 USA. [Martin, Melissa R.] US Fish & Wildlife Serv, ARM Loxahatchee Wildlife Refuge, Boynton Beach, FL USA. [Gettys, L. A.] Univ Florida, Ft Lauderdale Res & Educ Ctr, Davie, FL USA. RP Tipping, PW (reprint author), ARS, USDA, Invas Plant Res Lab, Davie, FL 33314 USA. EM philip.tipping@ars.usda.gov NR 33 TC 0 Z9 0 U1 1 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2015 VL 85 BP 12 EP 17 DI 10.1016/j.biocontrol.2015.03.001 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CH1QH UT WOS:000353795900002 ER PT J AU Lake, EC Smith, MC Dray, FA Pratt, PD AF Lake, Ellen C. Smith, Melissa C. Dray, F. A., Jr. Pratt, Paul D. TI Ecological host-range of Lilioceris cheni (Coleoptera: Chrysomelidae), a biological control agent of Dioscorea bulbifera SO BIOLOGICAL CONTROL LA English DT Article DE Field host-specificity; Herbivory; Dioscorea bulbifera; Lilioceris cheni; Spillover damage; Air potato ID LOOSESTRIFE LYTHRUM-SALICARIA; AIR-POTATO; GALERUCELLA-CALMARIENSIS; NONTARGET PLANTS; FIELD-TEST; SPECIFICITY; SELECTION; L.; BEHAVIOR; INSECTS AB Open-field host-specificity testing assesses the host-range of a biological control agent in a setting that permits the agent to use its full complement of host-seeking behaviors. This form of testing, particularly when it includes a no-choice phase in which the target weed is killed, may provide the most accurate assessment of the ecological host-range of an agent. We conducted a two-phase field host-specificity test with experienced and naive adults of Lilioceris cheni Gressitt and Kimoto (Coleoptera: Chrysomelidae), a biological control agent of Dioscorea bulbifera L. (Dioscoreales: Dioscoreaceae). We followed field tests with a no-choice laboratory consumption study with the congeneric plant species that received test feeding in the field, and an additional field evaluation of spillover risk. Both experienced and naive adults strongly preferred D. bulbifera to non-targets in the field. Within 47 h post-release, 90% of the released beetles that remained in the plots were found on D. bulbifera. In the laboratory no-choice test, the beetles consumed significantly more D. bulbifera and survived longer on this plant than the non-targets. All naive beetles in the Dioscorea sansibarensis and Dioscorea villosa treatments and 75% of naive beetles on Dioscorea floridana died within 7 d. Potted plants of the native D. floridana experienced minor test feeding in the spillover experiment when surrounded by large populations of L. cheni in the field. At the end of this experiment, L. cheni eggs and/or larvae were present on 83% of D. bulbifera plants but none of the D. floridana plants. We conclude that L. cheni is host-specific to D. bulbifera and does not pose a spillover risk to the native D. floridana. Published by Elsevier Inc. C1 [Lake, Ellen C.; Smith, Melissa C.; Dray, F. A., Jr.; Pratt, Paul D.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. RP Lake, EC (reprint author), ARS, USDA, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM ellen.lake@ars.usda.gov NR 39 TC 0 Z9 0 U1 2 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2015 VL 85 BP 18 EP 24 DI 10.1016/j.biocontrol.2014.12.021 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CH1QH UT WOS:000353795900003 ER PT J AU Behmke, S Fallon, J Duerr, AE Lehner, A Buchweitz, J Katzner, T AF Behmke, Shannon Fallon, Jesse Duerr, Adam E. Lehner, Andreas Buchweitz, John Katzner, Todd TI Chronic lead exposure is epidemic in obligate scavenger populations in eastern North America SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Chronic lead exposure; Lead ammunition; Scavenger; Vulture ID TURKEY VULTURES; AMMUNITION; CHILDREN; ELEMENTS; MERCURY; DISEASE; BLACK; USA AB Lead is a prominent and highly toxic contaminant with important impacts to wildlife. To understand the degree to which wildlife populations are chronically exposed, we quantified lead levels within American black vultures (Coragyps atratus; BLVU) and turkey vultures (Cathartes aura; TUVU), two species that are useful as environmental sentinels in eastern North America. Every individual sampled (n = 108) had bone lead levels indicative of chronic exposure to anthropogenic lead (BLVU:(x) over bar = 36.99 +/- 55.21 mg Pb/kg tissue (+/- SD); TUVU: (x) over bar = 23.02 +/- 18.77 mg/kg). Only a few showed evidence of recent lead exposure (BLVU liver: (x) over bar = 0.78 +/- 0.93 mg/kg; TUVU liver:(x) over bar = 0.55 +/- 0.34 mg/kg). Isotopic ratios suggested multiple potential sources of lead including ammunition, gasoline, coal-fired power plants, and zinc smelting. Black and turkey vultures range across eastern North America, from Quebec to Florida and individuals may traverse thousands of kilometers annually. The extent to which vultures are exposed suggests that anthropogenic lead permeates eastern North American ecosystems to a previously unrecognized degree. Discovery of an epidemic of chronic lead exposure in such widespread and common species and the failure of soft-tissue sampling to diagnose this pattern has dramatic implications for understanding modern wildlife and human health concerns. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Behmke, Shannon; Duerr, Adam E.; Katzner, Todd] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Fallon, Jesse] Virginia Polytech Univ, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Lehner, Andreas; Buchweitz, John] Michigan State Univ, Dept Pathobiol & Diagnost Invest, Lansing, MI 48910 USA. [Katzner, Todd] US Forest Serv, USDA, No Res Stn, Elkins, WV 26287 USA. RP Behmke, S (reprint author), W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM sbehmke@mix.wvu.edu; tkatzner@usgs.gov OI Katzner, Todd/0000-0003-4503-8435 FU Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Restoration grant from the U.S. Fish and Wildlife Service [2013-14308]; West Virginia University - Peace Corps Master's International Program FX Vulture carcasses were graciously provided by the Virginia State Office of USDA Wildlife Services. Pat Mazik and Scott Barras provided helpful reviews of the manuscript and Glenna Schmidt and Erin Katzner assisted with necropsies. This publication was completed with funds in part provided by the Virginia Department of Game and Inland Fisheries through a Federal Aid in Wildlife Restoration grant from the U.S. Fish and Wildlife Service (contract #2013-14308) and the West Virginia University - Peace Corps Master's International Program. This Scientific Article No. 3241 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. NR 33 TC 4 Z9 5 U1 1 U2 37 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 EI 1873-6750 J9 ENVIRON INT JI Environ. Int. PD JUN PY 2015 VL 79 BP 51 EP 55 DI 10.1016/j.envint.2015.03.010 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA CH2JZ UT WOS:000353852800006 PM 25795925 ER PT J AU Cabrera, AR Shirk, PD Evans, JD Hung, K Sims, J Alborn, H Teal, PEA AF Cabrera, A. R. Shirk, P. D. Evans, J. D. Hung, K. Sims, J. Alborn, H. Teal, P. E. A. TI Three Halloween genes from the Varroa mite, Varroa destructor (Anderson & Trueman) and their expression during reproduction SO INSECT MOLECULAR BIOLOGY LA English DT Article DE Apis mellifera; 20-hydroxyecdysone; ponasterone A; ecdysteroids; spook; disembodied; shade; CYP307a1; CYP302a1; CYP314a1 ID DROSOPHILA-MELANOGASTER; ECDYSTEROID BIOSYNTHESIS; ECDYSONE BIOSYNTHESIS; AMBLYOMMA-HEBRAEUM; MOLTING HORMONE; LARVAL CUTICLE; ZYGOTIC LOCI; P450 ENZYMES; HONEY-BEE; CYTOCHROME-P450 ENZYME AB The ecdysteroid biosynthetic pathway involves sequential enzymatic hydroxylations by a group of enzymes collectively known as Halloween gene proteins. Complete sequences for three Halloween genes, spook (Vdspo), disembodied (Vddib) and shade (Vdshd), were identified in varroa mites and sequenced. Phylogenetic analyses of predicted amino acid sequences for Halloween orthologues showed that the acarine orthologues were distantly associated with insect and crustacean clades indicating that acarine genes had more ancestral characters. The lack of orthologues or pseudogenes for remaining genes suggests these pathway elements had not evolved in ancestral arthropods. Vdspo transcript levels were highest in gut tissues, while Vddib transcript levels were highest in ovary-lyrate organs. In contrast, Vdshd transcript levels were lower overall but present in both gut and ovary-lyrate organs. All three transcripts were present in eggs removed from gravid female mites. A brood cell invasion assay was developed for acquiring synchronously staged mites. Mites within 4h of entering a brood cell had transcript levels of all three that were not significantly different from mites on adult bees. These analyses suggest that varroa mites may be capable of modifying 7-dehydro-cholesterol precursor and hydroxylations of other steroid precursors, but whether the mites directly produce ecdysteroid precursors and products remains undetermined. C1 [Cabrera, A. R.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Shirk, P. D.; Hung, K.; Sims, J.; Alborn, H.; Teal, P. E. A.] USDA ARS, CMAVE, Gainesville, FL 32608 USA. [Evans, J. D.] USDA ARS, BRL, BARC E, Beltsville, MD 20705 USA. RP Shirk, PD (reprint author), USDA ARS, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM paul.shirk@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 NR 58 TC 6 Z9 6 U1 4 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD JUN PY 2015 VL 24 IS 3 BP 277 EP 292 DI 10.1111/imb.12155 PG 16 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CG9NQ UT WOS:000353643000001 PM 25488435 ER PT J AU Raphael, MG Shirk, AJ Falxa, GA Pearson, SF AF Raphael, Martin G. Shirk, Andrew J. Falxa, Gary A. Pearson, Scott F. TI Habitat associations of marbled murrelets during the nesting season in nearshore waters along the Washington to California coast SO JOURNAL OF MARINE SYSTEMS LA English DT Article DE Boosted regression; Central place foraging; Foraging habitat; Habitat selection; Hotspot; Marbled murrelet; Nesting habitat; Pacific Northwest; USA; Sea surface temperature ID UPWELLING SYSTEM; BRACHYRAMPHUS-MARMORATUS; NORTHERN CALIFORNIA; SOUTHEAST ALASKA; SEABIRD; PACIFIC; CLIMATE; OCEAN; PREY; PREDATORS AB The marbled murrelet (Brachyramphus marmoratus) is a seabird in the family Alcidae that forages in nearshore waters of the Pacific Northwest, and nests in adjacent older-forest conifers within 80 km offshore. The species is of conservation concern due to habitat loss and declining numbers, and is listed as Threatened in British Columbia, Canada and in the United States portion of its range south of Canada. Recent monitoring in the United States indicated that murrelet numbers continued to decline there, especially in the waters of Washington State. To better understand this decline, and to inform conservation planning for the species, we evaluated how terrestrial and marine factors influence the distribution and abundance of the murrelet in coastal waters, including whether at-sea hotspots of murrelet abundance exist. Murrelet at-sea abundance and distribution were determined by surveys conducted annually from 2000 to 2012 in coastal waters from the United States-Canada border south to San Francisco Bay. We summarized mean and variance of murrelet density at the scale of 5-km segments of coastal waters throughout this area. We used a boosted regression tree analysis to investigate the contributions of a suite of marine and terrestrial attributes to at-sea murrelet abundance in each segment. We observed several regional hotspots of higher murrelet abundance at sea. Terrestrial attributes made the strongest contribution, especially the amount and cohesiveness of suitable nesting habitat in proximity to each segment, whereas marine attributes explained less of the spatial and temporal variations in murrelet abundance. At-sea hotspots of murrelet abundance therefore reflect not only suitable marine foraging habitat but primarily the proximity of suitable inland nesting habitat. Published by Elsevier B.V. C1 [Raphael, Martin G.] USDA, Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. [Shirk, Andrew J.] Univ Washington, Coll Environm, Climate Impacts Grp, Seattle, WA 98195 USA. [Falxa, Gary A.] US Fish & Wildlife Serv, Arcata Fish & Wildlife Off, Arcata, CA 95521 USA. [Pearson, Scott F.] Washington Dept Fish & Wildlife, Wildlife Sci Div, Olympia, WA 98501 USA. RP Raphael, MG (reprint author), USDA, Forest Serv, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM mraphael@fs.fed.us FU U.S. Fish and Wildlife Service; Bureau of Land Management; U.S. Forest Service; Pacific Northwest Research Station; federal Northwest Forest Plan Effectiveness Monitoring Program FX This study evolved from a larger effort to investigate the status and trend of the murrelet under the auspices of the federal Northwest Forest Plan Effectiveness Monitoring Program sponsored by the U.S. Fish and Wildlife Service, Bureau of Land Management, and U.S. Forest Service. We are indebted to other members of the monitoring team for insights and suggestions leading to this work, including M. Lance, L Long, D. Lynch, S.L. Miller, S.K. Nelson, C.J. Ralph, C.S. Strong, and R.D. Young. Funding for this study was contributed by the Pacific Northwest Research Station and the U.S. Fish and Wildlife Service. We thank the many field assistants who collected the data reported herein, as well as to S. Miller, C. Strong, L. Long, M. Lance, and T. Bloxton who led the field crews. We benefitted from statistical advice from J. Baldwin, A. Steele, and N. Povak. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service. NR 67 TC 5 Z9 6 U1 4 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0924-7963 EI 1879-1573 J9 J MARINE SYST JI J. Mar. Syst. PD JUN PY 2015 VL 146 SI SI BP 17 EP 25 DI 10.1016/j.jmarsys.2014.06.010 PG 9 WC Geosciences, Multidisciplinary; Marine & Freshwater Biology; Oceanography SC Geology; Marine & Freshwater Biology; Oceanography GA CG8ZP UT WOS:000353604500003 ER PT J AU O'Donnell, V Holinka, LG Gladue, DP Sanford, B Krug, PW Lu, XQ Arzt, J Reese, B Carrillo, C Risatti, GR Borca, MV AF O'Donnell, Vivian Holinka, Lauren G. Gladue, Douglas P. Sanford, Brenton Krug, Peter W. Lu, Xiqiang Arzt, Jonathan Reese, Bo Carrillo, Consuelo Risatti, Guillermo R. Borca, Manuel V. TI African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus SO JOURNAL OF VIROLOGY LA English DT Article ID MULTIGENE FAMILIES; DOMESTIC SWINE; GENOME; FAMILY-360; SEQUENCE; REPLICATION; DETERMINANT; MACROPHAGES; HOMOLOG; GROWTH AB African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. The control of African swine fever (ASF) has been hampered by the unavailability of vaccines. Experimental vaccines have been developed using genetically modified live attenuated ASFVs where viral genes involved in virus virulence were removed from the genome. Multigene family 360 (MGF360) and MGF505 represent a group of genes sharing partial sequence and structural identities that have been connected with ASFV host range specificity, blocking of the host innate response, and virus virulence. Here we report the construction of a recombinant virus (ASFV-G-Delta MGF) derived from the highly virulent ASFV Georgia 2007 isolate (ASFV-G) by specifically deleting six genes belonging to MGF360 or MGF505: MGF505-1R, MGF360-12L, MGF360-13L, MGF360-14L, MGF505-2R, and MGF505-3R. ASFV-G-Delta MGF replicates as efficiently in primary swine macrophage cell cultures as the parental virus. In vivo, ASFV-G-Delta MGF is completely attenuated in swine, since pigs inoculated intramuscularly (i.m.) with either 102 or 104 50% hemadsorbing doses (HAD(50)) remained healthy, without signs of the disease. Importantly, when these animals were subsequently exposed to highly virulent parental ASFV-G, no signs of the disease were observed, although a proportion of these animals harbored the challenge virus. This is the first report demonstrating the role of MGF genes acting as independent determinants of ASFV virulence. Additionally, ASFV-G-Delta MGF is the first experimental vaccine reported to induce protection in pigs challenged with highly virulent and epidemiologically relevant ASFV-G. IMPORTANCE The main problem for controlling ASF is the lack of vaccines. Studies focusing on understanding ASFV virulence led to the production of genetically modified recombinant viruses that, while attenuated, are able to confer protection in pigs challenged with homologous viruses. Here we have produced an attenuated recombinant ASFV derived from highly virulent ASFV strain Georgia (ASFV-G) lacking only six of the multigene family 360 (MGF360) and MGF505 genes (ASFV-G-Delta MGF). It is demonstrated, by first time, that deleting specific MGF genes alone can completely attenuate a highly virulent field ASFV isolate. Recombinant virus ASFV-G-Delta MGF effectively confers protection in pigs against challenge with ASFV-G when delivered once via the intramuscular (i.m.) route. The protection against ASFV-G is highly effective by 28 days postvaccination. This is the first report of an experimental vaccine that induces solid protection against virulent ASFV-G. C1 [O'Donnell, Vivian; Holinka, Lauren G.; Gladue, Douglas P.; Sanford, Brenton; Krug, Peter W.; Arzt, Jonathan; Borca, Manuel V.] USDA, Plum Isl Anim Dis Ctr, Agr Res Serv, Greenport, NY 11944 USA. [Carrillo, Consuelo] USDA, Plum Isl Anim Dis Ctr, APHIS, Greenport, NY USA. [Lu, Xiqiang] USDA ARS, Plum Isl Anim Dis Ctr, DHS, Greenport, NY 11944 USA. [O'Donnell, Vivian; Gladue, Douglas P.; Risatti, Guillermo R.] Univ Connecticut, CAHNR, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Reese, Bo] Univ Connecticut, Ctr Genome Innovat, Storrs, CT USA. RP Borca, MV (reprint author), USDA, Plum Isl Anim Dis Ctr, Agr Res Serv, Greenport, NY 11944 USA. EM manuel.borca@ars.usda.gov OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178; Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of U.S. Department of Homeland Security [HSHQDC-11-X-00077, HSHQPM-12-X-00005]; ARS/USDA-University of Connecticut (SCA) [58-1940-1-190] FX This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under award no. HSHQDC-11-X-00077 and HSHQPM-12-X-00005. We thank ARS/USDA-University of Connecticut (SCA no. 58-1940-1-190) for partially supporting this work. NR 37 TC 9 Z9 9 U1 0 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD JUN PY 2015 VL 89 IS 11 BP 6048 EP 6056 DI 10.1128/JVI.00554-15 PG 9 WC Virology SC Virology GA CH3DU UT WOS:000353907900026 PM 25810553 ER PT J AU Abdeen, Z Ramlawi, A Qaswari, R Abu Alrub, A Dary, O Rambeloson, Z Shahab-Ferdows, S Dror, D Allen, LH Carriquiry, A Salman, R Dkeidek, S AF Abdeen, Ziad Ramlawi, Asa'd Qaswari, Radwan Abu Alrub, Ala' Dary, Omar Rambeloson, Zo Shahab-Ferdows, Setareh Dror, Daphna Allen, Lindsay H. Carriquiry, Alicia Salman, Rand Dkeidek, Sahar TI Predicted efficacy of the Palestinian wheat flour fortification programme: complementary analysis of biochemical and dietary data SO PUBLIC HEALTH NUTRITION LA English DT Article DE Flour fortification; Nutritional status; Gaza; West Bank; Palestinian Authority ID VITAMIN-A; INTAKE DISTRIBUTIONS; FORTIFIED FLOURS; BREAD; POPULATION; DEFICIENCY; PROBABILITY; RIBOFLAVIN; STABILITY; THIAMINE AB Objective To utilize complementary biochemical and dietary data collected before the initiation of national flour fortification to (i) identify micronutrient insufficiencies or deficiencies and dietary inadequacies in Palestinian women and children in vulnerable communities and (ii) assess the suitability of the current wheat flour fortification formula. Design Quantitative dietary intake questionnaires were administered and fasting venous blood samples collected in randomly selected households in Gaza City and Hebron. The impact of fortification was simulated by estimating the additional micronutrient content of fortified wheat flour. Setting Households in Gaza City and Hebron that were not receiving food aid from social programmes. Subjects Non-pregnant women (18-49 years) and children aged 36-83 months. Results The micronutrients with highest prevalence of insufficiency were vitamin D in women (84-97 % with serum 25-hydroxyvitamin D<50 nmol/l) and vitamin B-12 in women and children (43-82 % with serum B-12<221 pmol/l). Deficiencies of vitamin A, Fe and Zn were also of public health concern. Current levels of wheat flour fortificants were predicted to improve, but not eliminate, micronutrient intake inadequacies. Modification of fortificant concentrations of vitamin D, thiamin, vitamin B-12, Zn and folic acid may be indicated. Conclusions Micronutrient insufficiencies or deficiencies and intake inadequacies were prevalent based on either biochemical or dietary intake criteria. Adjustments to the current fortification formula for wheat flour are necessary to better meet the nutrient needs of Palestinian women and children. C1 [Abdeen, Ziad; Qaswari, Radwan; Dkeidek, Sahar] Al Quds Univ, Nutr & Hlth Res Inst, Jerusalem, Israel. [Ramlawi, Asa'd; Abu Alrub, Ala'] Minist Hlth, Palestinian Author, Ramallah, West Bank, Israel. [Dary, Omar; Rambeloson, Zo; Salman, Rand] A2Z USAID Project Micronutrients & Child Blindnes, Washington, DC USA. [Shahab-Ferdows, Setareh; Dror, Daphna; Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Carriquiry, Alicia] Iowa State Univ, Dept Stat, Ames, IA USA. RP Dary, O (reprint author), US Agcy Int Dev, Bur Global Hlth HIDN, Ronald Reagan Bldg 3-7-40,1300 Penn Ave NW, Washington, DC 20523 USA. EM DaryPHN@gmail.com FU US Agency for International Development (USAID) [GHS-A-00-05-00012-00] FX This article was made possible by the generous support of the US Agency for International Development (USAID) under the terms of Cooperative Agreement No. GHS-A-00-05-00012-00 to FHI-360 (and before to AED). USAID had no role in the design, analysis, or writing of this article. NR 30 TC 1 Z9 1 U1 1 U2 7 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD JUN PY 2015 VL 18 IS 8 BP 1358 EP 1368 DI 10.1017/S1368980014001554 PG 11 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CG7WW UT WOS:000353518800004 PM 25171194 ER PT J AU Diep, CS Beltran, A Chen, TA Thompson, D O'Connor, T Hughes, S Baranowski, J Baranowski, T AF Diep, Cassandra S. Beltran, Alicia Chen, Tzu-An Thompson, Debbe O'Connor, Teresia Hughes, Sheryl Baranowski, Janice Baranowski, Tom TI Predicting use of effective vegetable parenting practices with the Model of Goal Directed Behavior SO PUBLIC HEALTH NUTRITION LA English DT Article DE Vegetables; Parenting practices; Theory; Pre-school child ID PLANNED BEHAVIOR; CHILDRENS FRUIT; SCALE DEVELOPMENT; SELF-EFFICACY; CONSUMPTION; PREVENTION; VALIDITY; CANCER; METAANALYSIS; ADOLESCENTS AB Objective:To model effective vegetable parenting practices using the Model of Goal Directed Vegetable Parenting Practices construct scales. Design: An Internet survey was conducted with parents of pre-school children to assess their agreement with effective vegetable parenting practices and Model of Goal Directed Vegetable Parenting Practices items. Block regression modelling was conducted using the composite score of effective vegetable parenting practices scales as the outcome variable and the Model of Goal Directed Vegetable Parenting Practices constructs as predictors in separate and sequential blocks: demographics, intention, desire (intrinsic motivation), perceived barriers, autonomy, relatedness, self-efficacy, habit, anticipated emotions, perceived behavioural control, attitudes and lastly norms. Backward deletion was employed at the end for any variable not significant at P<0.05. Setting: Houston, TX, USA. Subjects: Three hundred and seven parents (mostly mothers) of pre-school children. Results: Significant predictors in the final model in order of relationship strength included habit of active child involvement in vegetable selection, habit of positive vegetable communications, respondent not liking vegetables, habit of keeping a positive vegetable environment and perceived behavioural control of having a positive influence on child's vegetable consumption. The final model's adjusted R-2 was 0.486. Conclusions: This was the first study to test scales from a behavioural model to predict effective vegetable parenting practices. Further research needs to assess these Model of Goal Directed Vegetable Parenting Practices scales for their (i) predictiveness of child consumption of vegetables in longitudinal samples and (ii) utility in guiding design of vegetable parenting practices interventions. C1 [Diep, Cassandra S.; Beltran, Alicia; Chen, Tzu-An; Thompson, Debbe; O'Connor, Teresia; Hughes, Sheryl; Baranowski, Janice; Baranowski, Tom] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. [Diep, Cassandra S.] Baylor Coll Med, Dept Family & Community Med, Houston, TX 77030 USA. RP Diep, CS (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM cassandra.diep@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Institute of Child Health and Human Development [HD058175]; USDA/ARS [58-6250-6001]; National Research Service Award [T32 HP10031] FX This research was funded by the National Institute of Child Health and Human Development (grant number HD058175). This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and was funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-6001. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products or organizations imply endorsement from the US government. C.S.D. was supported fully by a Primary Care Research Training Grant from National Research Service Award (#T32 HP10031). No funder had a role in the design, analysis or writing of this article. NR 53 TC 1 Z9 1 U1 4 U2 9 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD JUN PY 2015 VL 18 IS 8 BP 1389 EP 1396 DI 10.1017/S1368980014002079 PG 8 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA CG7WW UT WOS:000353518800007 PM 25234656 ER PT J AU Zhang, JZ Gu, F Zhu, JY Zalesny, RS AF Zhang, Jingzhi Gu, Feng Zhu, J. Y. Zalesny, Ronald S., Jr. TI Using a combined hydrolysis factor to optimize high titer ethanol production from sulfite-pretreated poplar without detoxification SO BIORESOURCE TECHNOLOGY LA English DT Article DE Poplar woods; Cellulosic biofuel; High solids processing; Fermentation inhibition; Pretreatment optimization ID ENHANCE ENZYMATIC SACCHARIFICATION; GENETICALLY-ENGINEERED STRAIN; DILUTE-ACID PRETREATMENTS; ACETIC-ACID; SACCHAROMYCES-CEREVISIAE; OVERCOME RECALCITRANCE; INHIBITORY COMPOUNDS; SPORL PRETREATMENTS; LODGEPOLE PINE; HIGH SOLIDS AB Sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL) was applied to poplar NE222 chips in a range of chemical loadings, temperatures, and times. The combined hydrolysis factor (CHF) as a pretreatment severity accurately predicted xylan dissolution by SPORL. Good correlations between CHF and pretreated solids enzymatic digestibility, sugar yield, and the formations of furfural and acetic acid were obtained. Therefore, CHF was used to balance sugar yield with the formation of fermentation inhibitors for high titer ethanol production without detoxification. The results indicated that optimal sugar yield can be achieved at CHF = 3.1, however, fermentation using un-detoxified whole slurries of NE222 pretreated at different severities by SPORL indicated CHF approximate to 2 produced best results. An ethanol titer of 41 g/L was achieved at total solids of approximately 20 wt% without detoxification with a low cellulase loading of 15 FPU/g glucan (27 mL/kg untreated wood). Published by Elsevier Ltd. C1 [Zhang, Jingzhi] Beijing Univ Chem Technol, Sch Life Sci Technol, Beijing 100029, Peoples R China. [Zhang, Jingzhi; Gu, Feng; Zhu, J. Y.] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Gu, Feng] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing, Jiangsu, Peoples R China. [Zalesny, Ronald S., Jr.] USDA Forest Serv, No Res Stn, Rhinelander, WI USA. RP Zhu, JY (reprint author), USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU USDA Small Business Innovative Research (SBIR) Phase II project [2010-33610-21589]; Chinese Scholarship Council (CSC); USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (AFRI) Competitive grant through the Northwest Advanced Renewables Alliance (NARA) [2011-68005-30416] FX This work was supported by a USDA Small Business Innovative Research (SBIR) Phase II project (Contract No.: 2010-33610-21589) to Biopulping International, the Chinese Scholarship Council (CSC), and USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (AFRI) Competitive grant (No. 2011-68005-30416) through the Northwest Advanced Renewables Alliance (NARA). The funding from these programs made the visiting appointment of Zhang at the USDA Forest Products Laboratory (FPL) possible. We would like to acknowledge Fred Matt of US Forest Service Forest Products Laboratory for conducting carbohydrate analyses, Drs. Xuejun Pan of University of Wisconsin-Madison and Bruce Dien of USDA Agricultural Research Service, National Center for Agriculture Utilization Research, for providing insightful discussions. NR 33 TC 8 Z9 8 U1 3 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUN PY 2015 VL 186 BP 223 EP 231 DI 10.1016/j.biortech.2015.03.080 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA CG3PA UT WOS:000353190900029 PM 25817033 ER PT J AU Worqlul, AW Collick, AS Rossiter, DG Langan, S Steenhuis, TS AF Worqlul, Abeyou W. Collick, Amy S. Rossiter, David G. Langan, Simon Steenhuis, Tammo S. TI Assessment of surface water irrigation potential in the Ethiopian highlands: The Lake Tana Basin SO CATENA LA English DT Article DE Low flows; East Africa; Horn of Africa; Surface water availability; Ethiopia; Surface irrigation ID BALANCE AB Although Ethiopia has a large potential to develop surface irrigation, only 5% of the 30 to 70 million hectares (ha) potentially available has been developed. To examine the underlying causes of this lack of irrigation development, this study evaluates the suitability of surface water irrigation for the Lake Tana Basin development corridor. Surface water availability and land potentially suitable for irrigation development were considered. Surface water potential was examined by analyzing long-term daily historical river discharges. Land suitable for irrigation was determined with a GIS-based multi-criteria evaluation, which considers the interaction of various factors such as climate, river proximity, soil type, land cover, topography/slope, and market outlets. The results indicate that nearly 20% of the Lake Tana Basin is suitable for surface irrigation. However, after analyzing 27 years of river discharge, less than 3% of the potential irrigable area (or less than 0.25% of the basin area) could be irrigated consistently with runoff from the river systems. Thus, the irrigation potential in the Lake Tana Basin can be met by increasing dry season flows, by improving upland infiltration, by supplying water from reservoirs, or by pumping water directly from Lake Tana. Published by Elsevier B.V. C1 [Worqlul, Abeyou W.; Rossiter, David G.; Steenhuis, Tammo S.] Cornell Univ, Ithaca, NY USA. [Worqlul, Abeyou W.; Steenhuis, Tammo S.] Bahir Dar Univ, Sch Civil & Water Resource Engn, Bahir Dar, Ethiopia. [Worqlul, Abeyou W.; Langan, Simon] IWMI, Addis Ababa, Ethiopia. [Collick, Amy S.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Collick, AS (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702, University Pk, PA 16802 USA. EM amy.collick@ars.usda.gov FU Norman E. Borlaug Leadership Enhancement in Agriculture Program - USAID [016258-68]; International Water Management Institute [SAP REF: 45-16214]; IWMI; Partnership for Enhanced Engagement in Research (PEER) Science [AID-OAA-A-11-00012] FX This research was supported by a graduate student fellowship for the senior author by the Norman E. Borlaug Leadership Enhancement in Agriculture Program (Award Number 016258-68) funded by the USAID and the International Water Management Institute (SAP REF: 45-16214). The National Meteorological Agency of Ethiopia and Ethiopian Ministry Water and Energy provided daily rainfall and discharge data free of charge. We would like to acknowledge IWMI's financial support for the research. The Partnership for Enhanced Engagement in Research (PEER) Science (grant number AID-OAA-A-11-00012) financed part of the research cost as well. NR 23 TC 1 Z9 1 U1 3 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD JUN PY 2015 VL 129 BP 76 EP 85 DI 10.1016/j.catena.2015.02.020 PG 10 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA CG2DS UT WOS:000353085700008 ER PT J AU Shrestha, B Anderson, TA Acosta-Martinez, V Payton, P Canas-Carrell, JE AF Shrestha, Babina Anderson, Todd A. Acosta-Martinez, Veronica Payton, Paxton Canas-Carrell, Jaclyn E. TI The influence of multiwalled carbon nanotubes on polycyclic aromatic hydrocarbon (PAH) bioavailability and toxicity to soil microbial communities in alfalfa rhizosphere SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Carbon nanotubes; Nanomaterials; Microbial toxicity; Polycyclic aromatic hydrocarbons ID CHIRONOMUS-PLUMOSUS LARVAE; BOUND PESTICIDE-RESIDUES; AMENDED ACTIVATED CARBON; BLACK CARBON; MANUFACTURED NANOPARTICLES; ORGANIC CONTAMINANTS; REDUCE PCB; SORPTION; ADSORPTION; BIOACCUMULATION AB Carbon nanotubes (CNTs) may affect bioavailability and toxicity of organic contaminants due to their adsorption properties. Recent studies have observed the influence of multiwalled carbon nanotubes (MWNTs) on the fate of polycyclic aromatic hydrocarbons (PAHs) and other organic contaminants. Greenhouse studies (49 d) were conducted with alfalfa plants in two different soil types. Four treatment conditions (0, 25, 50, or 100 mg/kg MWNTs + 100 mg/kg PAHs mixture-pyrene and phenanthrene) were tested in order to determine their effects on soil microbial community composition and PAH residues. Microbial community structure in the two highest treatments (50 mg/kg and 100 mg/kg MWNTs) showed a dramatic shift in the presence of MWNTs in sandy loam soil (1% organic matter) in comparison to the control (0 mg/kg MWNTs). Many microbial fatty acid methyl ester (FAMEs) markers (i15:0, 16:1 omega 5c, 10Me17:0, 10Me16:0) were missing in the control soil. However, there was a lower abundance of these FAMEs in the 25 mg/kg MWNT treatment (except 10Me17:0) and a higher presence of these FAMES in the 50 mg/kg and 100 mg/kg MWNT treatments compared to control. In contrast, microbial community composition was not influenced by the MWNT treatments in sandy clay loam soil (5.9% organic matter). However, pyrene degradation in sandy clay loam soil significantly increased by 21% in the highest MWNT treatment group (100 mg/kg) and 9.34% in 50 mg/kg MWNT treatment. Under the conditions tested in this study, MWNTs significantly impacted the soil microbial community distribution and PAH degradation and effects were dependent on soil types, specifically organic matter content. (C) 2015 Elsevier Inc. All rights reserved. C1 [Shrestha, Babina; Anderson, Todd A.; Canas-Carrell, Jaclyn E.] Texas Tech Univ, Dept Environm Toxicol, Inst Environm & Human Hlth TIEHH, Lubbock, TX 79409 USA. [Acosta-Martinez, Veronica; Payton, Paxton] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX USA. [Shrestha, Babina] Pegasus Tech Serv Inc, Cincinnati, OH 45219 USA. RP Shrestha, B (reprint author), Pegasus Tech Serv Inc, 46 E Hollister St, Cincinnati, OH 45219 USA. EM shrestha.babina@epa.gov OI Anderson, Todd/0000-0001-8176-6339 NR 62 TC 4 Z9 8 U1 13 U2 87 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD JUN PY 2015 VL 116 BP 143 EP 149 DI 10.1016/j.ecoenv.2015.03.005 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA CG5EK UT WOS:000353313700019 PM 25800986 ER PT J AU Cheng, MY Dong, JH Lee, IM Bottner-Parker, KD Zhao, Y Davis, RE Laski, PJ Zhang, ZK McBeath, JH AF Cheng, Mingyuan Dong, Jiahong Lee, Ing-Ming Bottner-Parker, Kristi D. Zhao, Yan Davis, Robert E. Laski, Paul J. Zhang, Zhongkai McBeath, Jenifer Huang TI Group 16SrXII phytoplasma strains, including subgroup 16SrXII-E ('Candidatus Phytoplasma fragariae') and a new subgroup, 16SrXII-I, are associated with diseased potatoes (Solanum tuberosum) in the Yunnan and Inner Mongolia regions of China SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE "stolbur"; Genetic diversity; Phytoplasma classification ID PURPLE TOP DISEASE; 16S RIBOSOMAL-RNA; SECY GENE-SEQUENCES; MOLECULAR CHARACTERIZATION; 1ST REPORT; FINER DIFFERENTIATION; RFLP ANALYSIS; CLASSIFICATION; YELLOWS; IDENTIFICATION AB In China, potato is widely cultivated economic crop. Recently, potato diseases with characteristic symptoms of phytoplasma infections were found in potato fields. In 2006 and 2007, samples exhibiting symptoms including rosette and upright growth, upward rolling, yellowing and purpling of leaves, shortened and thickened internodes and formation of aerial tubers were collected from plants in Yunnan and Inner Mongolia and analyzed for the presence of phytoplasmas. DNA was extracted from tissues of 63 symptomatic and 10 asymptomatic plants. Phytoplasma 16S rRNA was amplified by PCR with primer pair P1/P7, followed by nested PCR with P1A/P7A, P1A/16S-SR or R16F2n/R16R2n. Twenty nine symptomatic plants (46 %), but no asymptomatic plants, tested positive for phytoplasmas. Nested PCR products were cloned and sequenced. Sequence analysis indicated that the phytoplasmas from diseased potatoes shared 98.1-99.8 % similarity with 'Candidatus Phytoplasma fragariae' (16SrXII-E) and other strains in 16SrXII subgroups. RFLP and phylogenetic analyses also indicated that phytoplasmas of group 16SrXII were associated with phytoplasma infected potatoes in China; these strains are most closely related to subgroup 16SrXII-E. Our results showed that five strains belonged to 16SrXII-E; 11 strains were designated as a new 16SrXII subgroup, 16SrXII-I; and subgroup affiliations of all others were not determined. The genetic diversity of the strains was corroborated by sequence analysis of ribosomal protein genes, the elongation factor Tu gene (tuf) and the pre-protein translocase membrane subunit gene (secY). The results illustrated the complexity and diversity of phytoplasmas associated with potatoes in China. C1 [Cheng, Mingyuan; Laski, Paul J.; McBeath, Jenifer Huang] Univ Alaska Fairbanks, Plant Pathol & Biotechnol Lab, Agr & Forestry Expt Stn, Fairbanks, AK 99775 USA. [Dong, Jiahong; Zhang, Zhongkai] Yunnan Acad Agr Sci, Biotechnol & Genet Germplasm Inst, Yunnan Key Lab Agr Biotechnol, Kunming 650223, Peoples R China. [Lee, Ing-Ming; Bottner-Parker, Kristi D.; Zhao, Yan; Davis, Robert E.] USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP McBeath, JH (reprint author), Univ Alaska Fairbanks, Plant Pathol & Biotechnol Lab, Agr & Forestry Expt Stn, Fairbanks, AK 99775 USA. EM jhmcbeath@alaska.edu FU USDA-FAS; Technical Assistance for Specialty Crops Program; Yunnan Provincial Modern Agriculture for Potatoes Industrialization System [[2011]191]; Key Scientific and Technological Plan Project of Yunnan Province [2013ZA006] FX This project was supported by the USDA-FAS, Technical Assistance for Specialty Crops Program, the Yunnan Provincial Modern Agriculture for Potatoes Industrialization System ([2011]191), and Key Scientific and Technological Plan Project of Yunnan Province (2013ZA006). NR 44 TC 2 Z9 2 U1 0 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUN PY 2015 VL 142 IS 2 BP 305 EP 318 DI 10.1007/s10658-015-0616-9 PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CG7GP UT WOS:000353471200009 ER PT J AU Hickey, ME Richards, GP Lee, JL AF Hickey, M. E. Richards, G. P. Lee, J-L TI Development of a two-step, non-probed multiplex real- time PCR for surveilling Vibrio anguillarum in seawater SO JOURNAL OF FISH DISEASES LA English DT Article DE non-probed multiplex qPCR; seawater; thermal factors; Vibrio anguillarum; vibriosis ID POLYMERASE-CHAIN-REACTION; HEMOLYSIN GENES; FISH PATHOGEN; VIRULENCE; IDENTIFICATION; AMPLIFICATION; EMPA; FILLETS; CELLS; RPON AB Vibrio anguillarum is an aggressive and halophilic bacterial pathogen most commonly originating from seawater. Vibrio anguillarum presence in fisheries and aquaculture facilities causes significant morbidity and mortality among aquaculture species primarily from haemorrhaging of the body and skin of the infected fish that eventually leads to death, collectively recognized as the disease vibriosis. This study served to develop a non-probe, multiplex real-time PCR assay to rapidly detect V.anguillarum presence in seawater. Specific primers targeting genes vah1, empA and rpoN of V.anguillarum were selected for multiplex reaction among 11 different primer sets and the extension step was eliminated. Primer concentration, denaturation time as well as annealing time and temperature of DNA amplification were optimized, thus reducing reaction duration. The two-step, non-probed multiplex real-time PCR set forth by this study detects as little as 3CFUmL(-1) of V.anguillarum presence in sea water, without enrichment cultivation, in 70min with molecular precision and includes melting curve confirmation. C1 [Hickey, M. E.; Lee, J-L] Delaware State Univ, Dept Human Ecol, Coll Agr & Related Sci, Food Sci Program, Dover, DE 19901 USA. [Richards, G. P.] USDA ARS, Dover, DE USA. RP Lee, JL (reprint author), Delaware State Univ, Dept Human Ecol, Coll Agr & Related Sci, Food Sci Program, Dover, DE 19901 USA. EM jlee@desu.edu FU USDA National Institute of Food and Agriculture [2011-38821-30923] FX This project was supported by the 1890 Capacity Building Grants Program (Grant no. 2011-38821-30923) from the USDA National Institute of Food and Agriculture. NR 34 TC 0 Z9 0 U1 4 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD JUN PY 2015 VL 38 IS 6 BP 551 EP 559 DI 10.1111/jfd.12264 PG 9 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA CG4GC UT WOS:000353242400006 PM 25039502 ER PT J AU Di Giacomo, S Brito, BP Perez, AM Bucafusco, D Pega, J Rodriguez, L Borca, MV Perez-Filgueira, M AF Di Giacomo, S. Brito, B. P. Perez, A. M. Bucafusco, D. Pega, J. Rodriguez, L. Borca, M. V. Perez-Filgueira, M. TI Heterogeneity in the Antibody Response to Foot-and-Mouth Disease Primo-vaccinated Calves SO Transboundary and Emerging Diseases LA English DT Article DE foot-and-mouth disease; antibody response; vaccine; cattle; breed ID DIRECT-CONTACT CHALLENGE; T-CELL DETERMINANTS; IMMUNE-RESPONSE; FMDV PEPTIDES; CORRELATION-COEFFICIENT; VIRUS PEPTIDE; CARRIER STATE; CATTLE; PROTECTION; POTENCY AB Foot-and-mouth disease (FMD) vaccines are routinely used as effective control tools in large regions worldwide and to limit outbreaks during epidemics. Vaccine-induced protection in cattle has been largely correlated with the FMD virus (FMDV)-specific antibodies. Genetic control of cattle immune adaptive responses has been demonstrated only for peptide antigens derived from FMDV structural proteins. Here, we quantify the heterogeneity in the antibody response of cattle primo-vaccinated against FMD and study its association with the genetic background in Holstein and Jersey sires. A total of 377 FMDV-seronegative calves (122 and 255 calves from 16 and 15 Holstein and Jersey sires, respectively) were included in the study. Samples were taken the day prior to primo-vaccination and 45days post-vaccination (dpv). Animals received commercial tetravalent FMD single emulsion oil vaccines formulated with inactivated FMDV. Total FMDV-specific antibody responses were studied against three viral strains included in the vaccine, and antibody titres were determined by liquid-phase blocking ELISA. Three linear hierarchical mixed regression models, one for each strain, were formulated to assess the heterogeneity in the immune responses to vaccination. The dependent variables were the antibody titres induced against each FMDV strain at 45dpv, whereas sire's breed' was included as a fixed effect, sire' was included as a random effect, and farm' was considered as a hierarchical factor to account for lack of independence of within herd measurements. A significant association was found between anti-FMDV antibody responses and sire's breed, with lower immune responses found in the Jersey sires' offspring compared with those from Holstein sires. No significant intrabreed variation was detected. In addition, farm management practices were similar in this study, and results of the serological assays were shown to be repeatable. It therefore seems plausible that differences in the immune response may be expected in the event of a mass vaccination campaigns. C1 [Di Giacomo, S.; Bucafusco, D.; Pega, J.; Perez-Filgueira, M.] Inst Nacl Tecnol Agr, CICVyA, Inst Virol, RA-1686 Hurlingham, Argentina. [Brito, B. P.; Perez, A. M.] Univ Calif Davis, Sch Vet Med, Ctr Anim Dis Modeling & Surveillance, Dept Med & Epidemiol, Davis, CA 95616 USA. [Perez, A. M.; Bucafusco, D.; Pega, J.; Perez-Filgueira, M.] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina. [Rodriguez, L.; Borca, M. V.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. RP Perez-Filgueira, M (reprint author), Inst Nacl Tecnol Agr, CICVyA, Inst Virol, N Repetto & De Los Reseros S-N, RA-1686 Hurlingham, Argentina. EM mperez@cnia.inta.gov.ar OI Borca, Manuel/0000-0002-0888-1178 FU Agricultural Research Service [58-1940-8-111F]; Instituto Nacional de Tecnologia Agropecuaria FMD Project [AESA-201721] FX This work was funded by the Agricultural Research Service Collaborative Agreement No. 58-1940-8-111F and the Instituto Nacional de Tecnologia Agropecuaria FMD Project No. AESA-201721. Authors want to acknowledge to Dr Sol Juncos for her expert veterinary assistance along the project, Dr Martin Peluca Ygartua for his assistance during sampling procedures and Dr Alejandra Capozzo for helpful discussions. Authors also thank Ms Melanie Prarat for critical reading and editing of the manuscript. Finally, our extended thanks to Mr Mariano Tuculet and Mr Francisco Furlong for their support and interest towards the project. NR 47 TC 1 Z9 1 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2015 VL 62 IS 3 BP 280 EP 287 DI 10.1111/tbed.12130 PG 8 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA CG8ML UT WOS:000353562000007 PM 23895140 ER PT J AU Root, HT Geiser, LH Jovan, S Neitlich, P AF Root, Heather T. Geiser, Linda H. Jovan, Sarah Neitlich, Peter TI Epiphytic macrolichen indication of air quality and climate in interior forested mountains of the Pacific Northwest, USA SO ECOLOGICAL INDICATORS LA English DT Article DE Air quality; Bioindicators; Climate change; Critical level; Lichen community; N deposition ID ATMOSPHERIC NITROGEN DEPOSITION; WESTERN UNITED-STATES; CRITICAL LOADS; LICHENS RESPOND; POLLUTION; WASHINGTON; OREGON; COMMUNITIES; MANAGEMENT; CALIFORNIA AB Biomonitoring can provide cost-effective and practical information about the distribution of nitrogen (N) deposition, particularly in regions with complex topography and sparse instrumented monitoring sites. Because of their unique biology, lichens are very sensitive bioindicators of air quality. Lichens lack a cuticle to control absorption or leaching of nutrients and they dynamically concentrate nutrients roughly in proportion to the abundance in the atmosphere. As N deposition increases, nitrogen-loving eutrophic lichens become dominant over oligotrophic lichens that thrive in nutrient-poor habitats. We capitalize on these characteristics to develop two lichen-based indicators of air-borne and depositional N for interior forested mountain ecosystems of the Pacific Northwest and calibrate them with N concentration measured in PM2.5 at 12 IMPROVE air quality monitoring sites in the study area. The two lichen indices and peak frequencies of individual species exhibited continuous relationships with inorganic N pollution throughout the range of N in ambient PM2.5, suggesting that the designation of a critical level or critical load is somewhat arbitrary because at any level above background, some species are likely to experience adverse impacts. The concentration of N in PM2.5 near the city of Spokane, Washington was the lowest measured at an instrumented monitoring site near known N pollution sources. This level, 0.37 mu g/m(3)/year, served as a critical level, corresponding to a concentration of 1.02% N in the lichen Letharia vulpina, which is similar to the upper end of background lichen N concentrations measured elsewhere in the western United States. Based on this level, we estimate critical loads to be 1.54 and 2.51 kg/ha/year of through-fall dissolved inorganic N deposition for lichen communities and lichen N concentration, respectively. We map estimated fine-particulate (PM2.5) N in ambient air based on lichen community and lichen N concentration indices to identify hotspots in the region. We also develop and map an independent lichen community-based bioclimatic index, which is strongly related to gradients in moisture availability and temperature variability. Lichen communities in the driest climates were more eutrophic than those in wetter climates at the same levels of N air pollution. Published by Elsevier Ltd. C1 [Root, Heather T.] Weber State Univ, Dept Bot, Ogden, UT 84408 USA. [Geiser, Linda H.] US Forest Serv Air Resource Management Program, Washington, DC 20250 USA. [Jovan, Sarah] USDA, Forest Serv, Portland Forestry Sci Lab, Portland, OR 97205 USA. [Neitlich, Peter] Natl Pk Serv, Winthrop, WA 98862 USA. RP Root, HT (reprint author), Weber State Univ, Dept Bot, 2504 Univ Circle, Ogden, UT 84408 USA. EM heatherroot@weber.edu; lgeiser@fs.fed.us; sjovan@fs.fed.us; peter.neitlich@gmail.com FU US Forest Service PNW Air Resource Management; Forest Inventory and Assessment FX We thank our field crews and numerous experts that assisted with fieldwork, identifications, and sample processing, especially Jim and Dottie Riley, Doug Glavich, Mark Boyll, Jennifer Ferriel, Larissa Lasselle, and Dave Lebo. We thank Roger Eliason and the UMN Research Analytical Lab for elemental analyses. FIA staff Andrew Gray, Karen Waddell, James Menlove, and Rhonda Mathison assisted with FIA data acquisition. The US Forest Service PNW Air Resource Management and Forest Inventory and Assessment programs funded sampling and analysis. NR 51 TC 4 Z9 4 U1 5 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD JUN PY 2015 VL 53 BP 95 EP 105 DI 10.1016/j.ecolind.2015.01.029 PG 11 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CF6JB UT WOS:000352661900011 ER PT J AU James, AM Burdett, C Mccool, MJ Fox, A Riggs, P AF James, A. M. Burdett, C. Mccool, M. J. Fox, A. Riggs, P. TI The geographic distribution and ecological preferences of the American dog tick, Dermacentor variabilis (Say), in the USA SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Dermacentor variabilis; ecological modelling; equine piroplasmosis; habitat suitability; landscape; maximum entropy; Rocky Mountain spotted fever ID HABITAT SUITABILITY; POPULATION-DYNAMICS; CLIMATE; MODELS; RANGE; NICHE; PREDICTION; IXODIDAE; SYSTEMS; EASTERN AB Equine piroplasmosis (EP), caused by two parasitic organisms, Theileria equi and Babesia caballi, is a tick-borne disease of recent concern in horses in the U.S.A. Outbreaks of EP have been detected in Florida, Missouri, Kansas and Texas. In 2009, EP transmission in Texas occurred through the adults of two tick species, Amblyomma mixtum [formerly known as Amblyomma cajennense (Fabricius, 1787)] Koch (Ixodida: Ixodidae) and Dermacentor variabilis (Say) (Ixodida: Ixodidae), the American dog tick (ADT). In this study, we developed a continent-scale map for the distribution of the EP vector species D.variabilis, using a presence-only modelling approach to assess the habitat preferences of this tick. We used identification records from our tick geodatabase of locations in which the presence of the ADT had been noted. The potential distribution of the ADT in the U.S.A. was estimated from environmental factors using the maximum entropy approach based on localities in which there is a high probability of occurrence according to habitat suitability. Elevation and temperature were found to be biologically significant environmental variables influencing the presence of this tick species. Properly designed and constructed probability surfaces using maximum entropy offer one useful approach to the mapping of distribution ranges of tick species based on suitable habitat in the U.S.A. C1 [James, A. M.; Mccool, M. J.; Fox, A.; Riggs, P.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ft Collins, CO 80524 USA. [Burdett, C.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. RP James, AM (reprint author), US Anim & Plant Hlth Inspect Serv, Ctr Epidemiol & Anim Hlth, USDA, Vet Serv, Bldg B,2150 Ctr Ave, Ft Collins, CO 80524 USA. EM Angela.M.James@aphis.usda.gov NR 34 TC 4 Z9 4 U1 6 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD JUN PY 2015 VL 29 IS 2 BP 178 EP 188 DI 10.1111/mve.12099 PG 11 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CF8FH UT WOS:000352791000008 PM 25684582 ER PT J AU Barber, LB Loyo-Rosales, JE Rice, CP Minarik, TA Oskouie, AK AF Barber, Larry B. Loyo-Rosales, Jorge E. Rice, Clifford P. Minarik, Thomas A. Oskouie, Ali K. TI Endocrine disrupting alkylphenolic chemicals and other contaminants in wastewater treatment plant effluents, urban streams, and fish in the Great Lakes and Upper Mississippi River Regions SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Ethylenediaminetetraacetic acid; 4-Nonylphenol compounds; 4-Tert-octylphenol compounds; Bisphenol A; Triclosan; Trace elements; Gadolinium anomaly; Fish endocrine disruption ID SEWAGE-TREATMENT PLANT; MALE FATHEAD MINNOWS; CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY; WIDESPREAD SEXUAL DISRUPTION; TROUT ONCORHYNCHUS-MYKISS; CARP CYPRINUS-CARPIO; NONYLPHENOL-ETHOXYLATES; LIQUID-CHROMATOGRAPHY; ESTROGENIC ACTIVITY; RAINBOW-TROUT AB Urban streams are an integral part of the municipal water cycle and provide a point of discharge for wastewater treatment plant (WWTP) effluents, allowing additional attenuation through dilution and transformation processes, as well as a conduit for transporting contaminants to downstream water supplies. Domestic and commercial activities dispose of wastes down-the-drain, resulting in wastewater containing complex chemical mixtures that are only partially removed during treatment A key issue associated with WWTP effluent discharge into streams is the potential to cause endocrine disruption in fish. This study provides a long-term (1999-2009) evaluation of the occurrence of alkylphenolic endocrine disrupting chemicals (EDCs) and other contaminants discharged from WWTPs into streams in the Great Lakes and Upper Mississippi River Regions (Indiana, Illinois, Michigan, Minnesota, and Ohio). The Greater Metropolitan Chicago Area Waterways, Illinois, were evaluated to determine contaminant concentrations in the major WWTP effluents and receiving streams, and assess the behavior of EDCs from their sources within the sewer collection system, through the major treatment unit processes at a WWTP, to their persistence and transport in the receiving stream. Water samples were analyzed for alkylphenolic EDCs and other contaminants, including 4-nonylphenol (NP), 4-nonylphenolpolyethoxylates (NPEO), 4-nonylphenolethoxycarboxylic acids (NPEC), 4-tert-octylphenol (OP), 4-tert-octylphenolpolyethoxylates (OPEO), bisphenol A, triclosan, ethylenediaminetetraacetic acid (EDTA), and trace elements. All of the compounds were detected in all of the WWTP effluents, with EDTA and NPEC having the greatest concentrations. The compounds also were detected in the WWTP effluent dominated rivers. Multiple fish species were collected from river and lake sites and analyzed for NP, NPEO, NPEC, OP, and OPEO. Whole-body fish tissue analysis indicated widespread occurrence of alkylphenolic compounds, with the highest concentrations occurring in streams with the greatest WWTP effluent content. Biomarkers of endocrine disruption in the fish indicated long-term exposure to estrogenic chemicals in the wastewater impacted urban waterways. Published by Elsevier B.V. C1 [Barber, Larry B.] US Geol Survey, Boulder, CO 80303 USA. [Loyo-Rosales, Jorge E.] Ryerson Univ, Dept Biol & Chem, Toronto, ON M5B 2K3, Canada. [Rice, Clifford P.] USDA ARS, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [Minarik, Thomas A.; Oskouie, Ali K.] Metropolitan Water Reclamat Dist Greater Chicago, Cicero, IL 60804 USA. [Oskouie, Ali K.] IIT, Dept Civil Architectural & Environm Engn, Chicago, IL 60616 USA. RP Barber, LB (reprint author), US Geol Survey, 3215 Marine St, Boulder, CO 80303 USA. FU US Environmental Protection Agency under RARE [DW14921854-01-0] FX The authors gratefully acknowledge Peter Howe, Al Alwan, and Todd Nettesheim (US Environmental Protection Agency, Region V) for helping make the various studies possible and Dr. David Lordi (Metropolitan Water Reclamation District of Greater Chicago) for providing site access to the Calumet WWTP. Thanks to Greg Brown, Howard Taylor, and Steffanie Keefe (US Geological Survey) for technical assistance with chemical and data analyses. We thank all of the WWTP operators for providing site access and assistance with sampling. This research was partially supported by the US Environmental Protection Agency under RARE Grant DW14921854-01-0. Use of trade or product names is for identification only and does not imply endorsement by the US Government. NR 79 TC 13 Z9 15 U1 10 U2 112 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUN 1 PY 2015 VL 517 BP 195 EP 206 DI 10.1016/j.scitotenv.2015.02.035 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CF6JU UT WOS:000352663800019 PM 25727675 ER PT J AU Hughes, SO Power, TG O'Connor, TM Fisher, JO AF Hughes, Sheryl O. Power, Thomas G. O'Connor, Teresia M. Fisher, Jennifer Orlet TI Executive functioning, emotion regulation, eating self-regulation, and weight status in low-income preschool children: How do they relate? SO APPETITE LA English DT Article DE Hispanic preschoolers; Child eating self-regulation; Child weight status; Executive functioning; Emotional regulation; Delay of gratification ID BEHAVIOR QUESTIONNAIRE; INHIBITORY CONTROL; REGULATION SKILLS; EFFORTFUL CONTROL; EARLY-CHILDHOOD; YOUNG-CHILDREN; EXCESS WEIGHT; OBESE HUMANS; OVERWEIGHT; ABILITY AB The purpose of the present study was to examine relationships between child eating self-regulation, child non-eating self-regulation, and child BMIz in a low-income sample of Hispanic families with preschoolers. The eating in the absence of hunger task as well as parent-report of child satiety responsiveness and food responsiveness were used to assess child eating self-regulation. Two laboratory tasks assessing executive functioning, a parent questionnaire assessing child effortful control (a temperament dimension related to executive functioning), and the delay of gratification and gift delay tasks assessing child emotion regulation were used to assess child non-eating self-regulation. Bivariate correlations were run among all variables in the study. Hierarchical linear regression analyses assessed: (1) child eating selfregulation associations with the demographic, executive functioning, effortful control, and emotion regulation measures; and (2) child BMI z-score associations with executive functioning, effortful control, emotion regulation measures, and eating self-regulation measures. Within child eating self-regulation, only the two parent-report measures were related. Low to moderate positive correlations were found between measures of executive functioning, effortful control, and emotion regulation. Only three relationships were found between child eating self-regulation and other forms of child self-regulation: eating in the absence of hunger was positively associated with delay of gratification, and poor regulation on the gift delay task was associated positively with maternal reports of food responsiveness and negatively with parent-reports of satiety responsiveness. Regression analyses showed that child eating selfregulation was associated with child BMIz but other forms of child self-regulation were not. Implications for understanding the role of self-regulation in the development of child obesity are discussed. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Hughes, Sheryl O.; O'Connor, Teresia M.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Power, Thomas G.] Washington State Univ, Pullman, WA 99164 USA. [Fisher, Jennifer Orlet] Temple Univ, Ctr Obes Res & Educ, Philadelphia, PA 19140 USA. RP Hughes, SO (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates Ave, Houston, TX 77030 USA. EM shughes@bcm.edu FU National Institute of Child Health and Human Development [R01 HD062567]; US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, DO, USDA/ARS) [6250-51000] FX This research was supported by funds from the National Institute of Child Health and Human Development (Grant R01 HD062567). This work is also a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, DO, funded in part by the USDA/ARS (Cooperative Agreement 6250-51000). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. We would like to acknowledge the following people who were instrumental in collecting and coding the data for this study: Nilda Micheli, Monica Lopez, Ashley Beck, Veronica Bonilla-Pacheco, Rachael Hill, Yadi Olivera, Kayla Weinmann, and Noemi Aguilar. NR 69 TC 7 Z9 7 U1 15 U2 74 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD JUN 1 PY 2015 VL 89 BP 1 EP 9 DI 10.1016/j.appet.2015.01.009 PG 9 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA CF1SO UT WOS:000352328400001 PM 25596501 ER PT J AU Mueller-Warrant, GW Whittaker, GW Banowetz, GM Griffith, SM Barnhart, BL AF Mueller-Warrant, George W. Whittaker, Gerald W. Banowetz, Gary M. Griffith, Stephen M. Barnhart, Bradley L. TI Methods for improving accuracy and extending results beyond periods covered by traditional ground-truth in remote sensing classification of a complex landscape SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Agriculture; Ground-truth data; Landuse; Land cover; Object-based; Pixel-based; Majority-rule ID WESTERN OREGON; LANDSAT; NORTHWEST; DYNAMICS AB Successful development of approaches to quantify impacts of diverse landuse and associated agricultural management practices on ecosystem services is frequently limited by lack of historical and contemporary landuse data. We hypothesized that ground truth data from one year could be used to extrapolate previous or future landuse in a complex landscape where cropping systems do not generally change greatly from year to year because the majority of crops are established perennials or the same annual crops grown on the same fields over multiple years. Prior to testing this hypothesis, it was first necessary to classify 57 major landuses in the Willamette Valley of western Oregon from 2005 to 2011 using normal same year ground-truth, elaborating on previously published work and traditional sources such as Cropland Data Layers (CDL) to more fully include minor crops grown in the region. Available remote sensing data included Landsat, MODIS 16-day composites, and National Aerial Imagery Program (NAIP) imagery, all of which were resampled to a common 30 m resolution. The frequent presence of clouds and Landsat7 scan line gaps forced us to conduct of series of separate classifications in each year, which were then merged by choosing whichever classification used the highest number of cloud- and gap-free bands at any given pixel. Procedures adopted to improve accuracy beyond that achieved by maximum likelihood pixel classification included majority-rule reclassification of pixels within 91,442 Common Land Unit (CLU) polygons, smoothing and aggregation of areas outside the CLU polygons, and majority-rule reclassification over time of forest and urban development areas. Final classifications in all seven years separated annually disturbed agriculture, established perennial crops, forest, and urban development from each other at 90 to 95% overall 4-class validation accuracy. In the most successful use of subsequent year ground-truth data to classify prior year landuse, an overall 57-class accuracy of 75% was achieved despite the omission of 10 entire classes, most of which were annually disturbed or perennial crops grown on very few fields. Synthetic ground-truth data for the 2004 harvest year based on the most common landuse classes over the following 7 years classified 49 of 57 categories at an overall accuracy of 96% in a final version that included CLU polygon majority rule, default smoothing and aggregation, and forcing of urban development and forest from multi-year majority-rule. Published by Elsevier B.V. C1 [Mueller-Warrant, George W.; Whittaker, Gerald W.; Banowetz, Gary M.; Griffith, Stephen M.; Barnhart, Bradley L.] Agr Res Serv, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Mueller-Warrant, GW (reprint author), Agr Res Serv, USDA, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM George.Mueller-Warrant@ARS.USDA.GOV; whittakg@onid.orst.edu; banowetg@onid.orst.edu; griffits@onid.orst.edu; barnharb@onid.orst.edu FU USDA Cooperative State Research, Education, and Extension Service [2006-51130-03705] FX Contribution of USDA-ARS. The use of trade, firm, or corporation names in this publication (or page) is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. The authors wish to express their thanks to the USDA Cooperative State Research, Education, and Extension Service for funding of CEAP Special Grant No. 2006-51130-03705 "Assessing Trade-Offs Between Crop Production and Ecological Services: The Calapooia Basin" which was instrumental in our ability to conduct the research. NR 25 TC 5 Z9 5 U1 3 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD JUN PY 2015 VL 38 BP 115 EP 128 DI 10.1016/j.jag.2015.01.001 PG 14 WC Remote Sensing SC Remote Sensing GA CE6TC UT WOS:000351970100013 ER PT J AU Byars, JA Singh, M AF Byars, Jeffrey A. Singh, Mukti TI Properties of extruded chia-corn meal puffs SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Chia; Extrusion; Corn meal ID LIPID COMPLEX-FORMATION; SALVIA-HISPANICA L; EXTRUSION-COOKING; SUNFLOWER OILS; MODEL SYSTEM; WHEAT-STARCH; FLOUR; SNACKS; ACID; ARGENTINA AB This study investigated the properties of extruded corn meal puffs containing chia. Mixtures of corn meal and chia seeds (0-20 g/100 g) were processed in a laboratory-scale twin-screw extruder at different moisture contents (18-22 g/100 g) and final heating zone temperatures (120-160 degrees C). Extrusion processing provides a simple method for grinding the seeds, which is necessary for making the fatty acids available. The expansion of cylindrical extrudates decreased with increasing chia content, increasing moisture and increasing final heating zone temperature. The hardness of the extrudates increased with increasing moisture content and decreased at intermediate chia levels. The specific mechanical energy of the extrusion process decreased with increasing chia content. Published by Elsevier Ltd. C1 [Byars, Jeffrey A.; Singh, Mukti] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Byars, JA (reprint author), ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM jeffrey.byars@ars.usda.gov NR 21 TC 1 Z9 1 U1 4 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2015 VL 62 IS 1 SI SI BP 506 EP 510 DI 10.1016/j.lwt.2014.06.036 PN 2 PG 5 WC Food Science & Technology SC Food Science & Technology GA CE8SX UT WOS:000352115500019 ER PT J AU Raithore, S Dea, S Plotto, A Bai, JH Manthey, J Narciso, J Irey, M Baldwin, E AF Raithore, Smita Dea, Sharon Plotto, Anne Bai, Jinhe Manthey, John Narciso, Jan Irey, Mike Baldwin, Elizabeth TI Effect of blending Huanglongbing (HLB) disease affected orange juice with juice from healthy orange on flavor quality SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Huanglongbing; Sensory; Blending; Electronic tongue ID CITRUS; LIBERIBACTER; TEMPERATURE; THRESHOLDS; LIMONIN AB Huanglongbing, or citrus greening disease, progressively damages and ultimately kills citrus trees. While this disease does not affect human health, it is associated with bitter off-flavor for orange juice. The objective of this study was to determine the level of HLB-symptomatic (HLBs) juice (made from fruit symptomatic for the disease, harvested from diseased trees) that can be added to healthy juice (extracted from fruit harvested from healthy trees) so that the off-flavor associated with such juice is not perceived. Varying amounts of Hamlin or Valencia HLBs orange juices were blended with respective healthy juices to make 6.25, 12.5, 25, and 50 mL HLBs/100 mL blends. Chemical and sensorial differences were found for both varieties. HLBs juice had lower soluble solids but higher acidity, and limonin and nomilin levels than healthy juice. However, only about 27% of the panelists were able to recognize taste differences in 25 mL HLBS/100 mL blend and less than 9% could recognize taste differences with 6.25 and 12.5 mL HLBs/100 mL blends. Electronic tongue differentiation of blends supported the chemical and sensory differences. Results showed that adding small amounts of HLBs juice to healthy juice does not compromise taste quality. Published by Elsevier Ltd. C1 [Raithore, Smita; Dea, Sharon; Plotto, Anne; Bai, Jinhe; Manthey, John; Narciso, Jan; Baldwin, Elizabeth] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Irey, Mike] US Sugar Corp, Clewiston, FL 33440 USA. RP Baldwin, E (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM liz.baldwin@ars.usda.gov NR 26 TC 4 Z9 6 U1 2 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2015 VL 62 IS 1 SI SI BP 868 EP 874 DI 10.1016/j.lwt.2014.06.020 PN 2 PG 7 WC Food Science & Technology SC Food Science & Technology GA CE8SX UT WOS:000352115500069 ER PT J AU Glenn, EP Scott, RL Nguyen, U Nagler, PL AF Glenn, Edward P. Scott, Russell L. Uyen Nguyen Nagler, Pamela L. TI Wide-area ratios of evapotranspiration to precipitation in monsoon-dependent semiarid vegetation communities SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Remote sensing; Eddy covariance flux; MODIS; MODIS ET product; Enhanced vegetation index; Rangeland ecology; Shrub encroachment; Invasive grasses ID WOODY PLANT ENCROACHMENT; RITA-EXPERIMENTAL-RANGE; WATER-BALANCE; SHRUBLAND ECOSYSTEMS; CROP COEFFICIENTS; EDDY-COVARIANCE; WALNUT GULCH; FLUX TOWERS; GRASSLAND; MODIS AB Evapotranspiration (ET) and the ratio of ET to precipitation (PPT) are important factors in the water budget of semiarid rangelands and are in part determined by the dominant plant communities. Our goal was to see if landscape changes such as tree or shrub encroachment and replacement of native grasses by invasive grasses impacted ET and ET/PPT and therefore watershed hydrology in this biome. We determined ET and ET/PPT for shrublands, grasslands and mesquite savannas in southern Arizona at five moisture flux towers and determined the environmental factors controlling ET in each plant community. We then scaled ET over areas of 4-36 km(2), representing homogeneous patches of each plant community, using the Enhanced Vegetation Index (EVI) from MODIS sensors on the Terra satellite. Over wide areas, estimated ET/PPT projected from MODIS EVI ranged from 0.71 for a sparsely-vegetated shrub site to 1.00 for grasslands and mesquite savannas. The results did not support hypotheses that encroachment of mesquites into grasslands or that replacement of native grasses with introduced Eragrostis lehmanniana (lehmann lovegrass) have increased rangeland ET. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Glenn, Edward P.; Uyen Nguyen] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Scott, Russell L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Nagler, Pamela L.] US Geol Survey, Southwest Biol Sci Ctr, Sonoran Desert Res Stn, Tucson, AZ 85721 USA. RP Glenn, EP (reprint author), Univ Arizona, Dept Soil Water & Environm Sci, 1177 E 4th St,POB 210038, Tucson, AZ 85721 USA. EM eglenn@ag.arizona.edu; russ.scott@ars.usda.gov; uyenn@email.arizona.edu; pnagler@usgs.gov NR 75 TC 0 Z9 0 U1 3 U2 24 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD JUN PY 2015 VL 117 BP 84 EP 95 DI 10.1016/j.jaridenv.2015.02.010 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CE1GD UT WOS:000351559200010 ER PT J AU Collins, CDH Stone, JJ Cratic, L AF Collins, Chandra D. Holifield Stone, Jeffry J. Cratic, Leonard, III TI Runoff and sediment yield relationships with soil aggregate stability for a state-and-transition model in southeastern Arizona SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Soil aggregate stability; Erosion; Runoff; Ecological site; State-and-transition model ID EROSION; MANAGEMENT; ENVIRONMENT; RANGELANDS; LANDSCAPE; REMOVAL; QUALITY AB Soil erosion has been identified as the primary abiotic driver of site degradation on many semiarid rangelands. A key indicator of erosion potential that is being increasingly implemented in rangeland evaluations is soil aggregate stability (AS) as measured by a field soil slake test. However, there have been few studies that test if decreasing AS is an indication of increasing soil erosion. A rainfall simulator experiment was conducted in southeastern Arizona to measure runoff and erosion, aggregate stability, and cover attributes on three vegetation states of the state-and-transition model (STM) of the Loamy Upland ecological site (R041XC313AZ). The states included the reference state (RS), a site encroached by mesquite (MN), and a site invaded by Eragrostis lehmanniana (ML). Within the context of the STM, runoff was only different between very high and low cover states. Erosion and AS values differentiated among states, particularly between the RS and MN states. Relationships between runoff and erosion with canopy cover and interspace bare soil suggest that certain cover levels exist where runoff and erosion have the potential to increase. The results also indicated that for this ecological site, AS < 4 may represent an increased risk of erosion occurrence. Published by Elsevier Ltd. C1 [Collins, Chandra D. Holifield; Stone, Jeffry J.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Cratic, Leonard, III] Univ Arizona, Tucson, AZ 85721 USA. RP Collins, CDH (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM chandra.holifield@ars.usda.gov FU USDA-ARS Southwest Watershed Research Center FX Funding for this research was provided by the USDA-ARS Southwest Watershed Research Center. The authors would like to express sincere thanks to Bill Flack and all of the undergraduate and graduate students from the University of Arizona who served on the WG Rainfall Simulator crews over the years making it possible to accrue the extensive data sets used in this study, Emilio Carrillo from USDA-NRCS, the Empire Ranch managers, and the personnel at the USDA-ARS Southwest Watershed Research Center, Tucson, and the USDA-ARS Tombstone facility for their cooperation and assistance. NR 36 TC 5 Z9 5 U1 2 U2 23 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD JUN PY 2015 VL 117 BP 96 EP 103 DI 10.1016/j.jaridenv.2015.02.016 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CE1GD UT WOS:000351559200011 ER PT J AU Moser, BR Evangelista, RL Jham, G AF Moser, Bryan R. Evangelista, Roque L. Jham, Gulab TI Fuel properties of Brassica juncea oil methyl esters blended with ultra-low sulfur diesel fuel SO RENEWABLE ENERGY LA English DT Article DE Biodiesel; Blends; Brassica juncea; Diesel; Fatty acid methyl esters; Properties ID L. SEED OIL; BIODIESEL PRODUCTION; FATTY-ACIDS; MUSTARD; MEAL; SUPPRESSION; SOIL; FEEDSTOCKS; COSTS; YIELD AB Brassica juncea is a drought-tolerant member of the Brassicaceae plant family with high oil content and a short growing season that is tolerant of low quality soils. It was investigated as a feedstock for production of biodiesel along with evaluation of subsequent fuel properties, both neat and in blends with petroleum diesel fuel. These results were compared against relevant fuel standards such as ASTM D6751, EN 14214, ASTM D975, EN 590, and ASTM D7467. Crude B. juncea oil was extracted from unconditioned seeds utilizing a continuous tubular radial expeller. The oil was then chemically refined via degumming, neutralization and bleaching to render it amenable to direct homogeneous sodium methoxide-catalyzed transesterification. The principal fatty acid detected in B. juncea oil was erucic acid (44.1%). The resulting biodiesel yielded fuel properties compliant with the biodiesel standards with the exception of oxidative stability and kinematic viscosity in the case of EN 14214. Addition of tert-butylhydroquinone and blending with soybean oil-derived biodiesel ameliorated these deficiencies. The fuel properties of B5 and B20 blends of B. juncea oil methyl esters (BJME) in ultra-low sulfur (<15 ppm S) diesel (ULSD) fuel were within the ranges specified in the petrodiesel standards ASTM D975, EN 590 and ASTM D7467 with the exception of derived cetane number in the case of EN 590. This deficiency was attributed to the inherently low cetane number of the certification-grade ULSD, as it did not contain performance-enhancing additives. In summary, this study reports new fuel property data for BJME along with properties of B5 and B20 blends in ULSD. Such results will be useful for the development of B. juncea as an alternative source of biodiesel fuel. Published by Elsevier Ltd. C1 [Moser, Bryan R.; Evangelista, Roque L.] ARS, USDA, Natl Ctr Agr Utilizat Res, Biooils Res Unit, Peoria, IL 61604 USA. [Jham, Gulab] Univ Fed Vicosa, BR-36570000 Vicosa, MG, Brazil. RP Moser, BR (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov FU Brazilian government (CNPq - Conselho Nacional de Pesquisa) FX Kim Ascherl, Benetria Banks, Billy Deadmond, and Jeff Forrester are acknowledged for technical assistance. GJ acknowledges the Brazilian government (CNPq - Conselho Nacional de Pesquisa) for financial assistance. NR 30 TC 2 Z9 2 U1 2 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD JUN PY 2015 VL 78 BP 82 EP 88 DI 10.1016/j.renene.2015.01.016 PG 7 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA CE2LF UT WOS:000351646400010 ER PT J AU Rodgers, J Naylor, GRS Cui, XL Delhom, C Hinchliffe, D AF Rodgers, James Naylor, Geoffrey R. S. Cui, Xiaoliang Delhom, Chris Hinchliffe, Doug TI Cottonscope fiber maturity, fineness, and ribbon width measurements with different sample sizes SO TEXTILE RESEARCH JOURNAL LA English DT Article DE fiber; yarn; measurement; materials; properties; quality; cotton; image analysis ID LINEAR DENSITY FINENESS; IMAGE-ANALYSIS; LINT SAMPLES; INSTRUMENT AB The accurate and precise measurement of cotton maturity and fineness is often difficult, laborious, and/or expensive to perform. The Cottonscope (R) rapidly, accurately, and precisely measures fiber maturity and fineness with small quantities of sample (approximately 50mg). Interest has been expressed in the use of different sample weights of fiber, especially lower sample weights by geneticists. A program was implemented to establish the capabilities of the Cottonscope to rapidly and accurately measure maturity (maturity ratio (MR), original polarized light microscopy maturity ratio (MRBF)), fineness, and ribbon width with different sample weights of fiber. The change in sample weight significantly impacted the Cottonscope results for MR, fineness, and ribbon width. Fineness was most impacted by varied sample weight. The impacts of sample weights on the cotton results were removed by re-calibrating the instrument at each sample weight. Decreasing the sample weight below 50mg increased sample measurement times, especially at 10mg sample weight. These results demonstrated that the Cottonscope can accurately and precisely measure the fiber's maturity, fineness, and ribbon width with cotton fiber samples as small as 10mg. C1 [Rodgers, James; Cui, Xiaoliang; Delhom, Chris; Hinchliffe, Doug] ARS, SSRC, USDA, New Orleans, LA 70124 USA. [Naylor, Geoffrey R. S.] CSIRO, Mat Sci & Engn, Geelong, Vic, Australia. RP Rodgers, J (reprint author), ARS, SSRC, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM james.rodgers@ars.usda.gov FU Cotton Incorporated (Cary, NC) [58-6435-2-671] FX This research was partially supported by Cotton Incorporated (Cary, NC) under project number 58-6435-2-671. NR 27 TC 0 Z9 0 U1 4 U2 5 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 EI 1746-7748 J9 TEXT RES J JI Text. Res. J. PD JUN PY 2015 VL 85 IS 9 BP 897 EP 911 DI 10.1177/0040517514548809 PG 15 WC Materials Science, Textiles SC Materials Science GA CE3CZ UT WOS:000351703600001 ER PT J AU Hsu, H Sheen, S Sites, J Cassidy, J Scullen, B Sommers, C AF Hsu, HsinYun Sheen, Shiowshuh Sites, Joseph Cassidy, Jennifer Scullen, Butch Sommers, Christopher TI Effect of High Pressure Processing on the survival of Shiga Toxin-Producing Escherichia coli (Big Six vs. O157:H7) in ground beef SO FOOD MICROBIOLOGY LA English DT Article DE High pressure; Shiga toxin-producing Escherichia. coli; 'Big six' and O157:H7 STEC; Ground beef ID HIGH HYDROSTATIC-PRESSURE; LISTERIA-MONOCYTOGENES; SENSORY EVALUATION; MEAT-PRODUCTS; UNITED-STATES; INACTIVATION; PATTIES; O157-H7; PATHOGENS; BEHAVIOR AB High pressure processing (HPP) is a safe and effective technology for improving food safety. Non-O157:H7 Shiga Toxin-producing Escherichia coli (STEC) have been increasingly implicated in foodborne illness outbreaks and recalls, and the USDA Food Safety Inspection Service (FSIS) has designated them as adulterants in meat (e.g. ground beef). In this study we compared the inactivation of multi-isolate cocktails of E. coli O157:H7 versus the non-O157:H7 STEC "Big Six" (i.e. O26, O45, O103, O111, O121, and O145) in ground beef (83% lean) using HPP at refrigeration temperature (4-7 degrees C). A > 5-log CFU/g inactivation of both the Big Six and O157:H7 cocktails were observed at 450 MPa for 15 min. In general, the Big Six cocktail was found more sensitive to pressure stress (p < 0.05). In contrast, HPP treatment at 250 MPa (30 min) inactivated only 2.3 log of the Big Six versus 1.0 log of O157:H7. HPP treatment at 350 MPa (30 min) inactivated 4.7 log of the Big Six vs. 3.2 log of O157:H7. Multiple-cycle HPP cycles (250 or 350 MPa, three 5 min treatments) did not result in a 5 log reduction of the non-O157:H7 or O157:H7 STEC. Our results indicate that HPP inactivation parameters which are effective for O157:H7 STEC can be used for the non-O157:H7 Big Six isolates in ground beef. Published by Elsevier Ltd. C1 [Hsu, HsinYun] Natl Taiwan Univ, Taipei 10764, Taiwan. [Sheen, Shiowshuh; Sites, Joseph; Cassidy, Jennifer; Scullen, Butch; Sommers, Christopher] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov FU USDA/ARS/ERRC; National Science Council of Taiwan FX This project was supported by USDA/ARS/ERRC and National Science Council of Taiwan. NR 37 TC 10 Z9 10 U1 2 U2 57 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2015 VL 48 BP 1 EP 7 DI 10.1016/j.fm.2014.12.002 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CD7UQ UT WOS:000351299900001 PM 25790984 ER PT J AU Xu, SX Wang, DP Yang, D Liu, HS Tian, P AF Xu, Shuxia Wang, Dapeng Yang, David Liu, Huashan Tian, Peng TI Alternative methods to determine infectivity of Tulane virus: A surrogate for human nororvirus SO FOOD MICROBIOLOGY LA English DT Article DE Rapid assay; Tulane virus (TV); Human norovirus (HuNoV); Cellular culture mediated amplification qRT-PCR (CMA-qRT-PCR); TCID50; RNase exposure assay; In situ capture qRT-PCR (ISC-qRT-PCR); Inactivation status ID REVERSE TRANSCRIPTION-PCR; MURINE NOROVIRUS; FELINE CALICIVIRUS; INACTIVATION; TRANSFECTION; EXPOSURE; NORWALK AB Culturable animal caliciviruses are widely-used as surrogates for human norovirus (HuNoV). The infectivity of a culturable virus was traditionally determined by plaque assay and/or 50% tissue culture infectious dose (TCID50) assay, both of which are time-consuming and labor-intensive. Molecular approaches, such as quantitative real time RT-PCR (qRT-PCR) and RT-PCR, could be used for detection of the viral genome but yet fail to determine the infectivity of a virus. In this study, we evaluated different assays for determination of infectivity of Tulane virus (TV), a surrogate for HuNoV. The infectivity of TV was measured by RNase exposure assay, RT-PCR assays, cellular-receptor-mediated capture qRT-PCR assay, receptor-mediated in situ capture qRT-PCR assay, cell-culture-mediated amplification qRT-PCR, and confirmed by TCID50 assay. RNase exposure assay was only useful for measuring TV inactivation caused by heat. Short template RT-PCR assay did not reflect inactivation status of TV. Partial reduction in viral RNA signal could be measured by long-template RT-PCR only when TV was inactivated by thermal or chlorine treatments at full-inactivation levels. Cellular-receptor-mediated capture qRT-PCR exhibited low sensitivity and specificity for the evaluation of virus infectivity. The in situ capture qRT-PCR assay could be used to evaluate virus inactivation deriving from damage to viral capsid caused by heat and chlorine. The cell-culture-mediated amplification qRT-PCR could be used as an alternative method to rapidly determine the infectivity of TV. Published by Elsevier Ltd. C1 [Xu, Shuxia] Henan Agr Univ, Coll Agron, Zhengzhou 450002, Peoples R China. [Xu, Shuxia; Wang, Dapeng; Yang, David; Tian, Peng] USDA, ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. [Wang, Dapeng] Shanghai Jiao Tong Univ, Dept Food Sci & Technol, Shanghai 200240, Peoples R China. RP Xu, SX (reprint author), Henan Agr Univ, Coll Agron, 63 Nongye Rd, Zhengzhou 450002, Peoples R China. EM shuxiaxu97@gmail.com; peng.tian@ars.usda.gov FU USDA Agricultural Research Service CRIS project [5325-42000-046] FX This work was supported by USDA Agricultural Research Service CRIS project 5325-42000-046. USDA is an equal opportunity provider and employer. NR 12 TC 3 Z9 3 U1 3 U2 19 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2015 VL 48 BP 22 EP 27 DI 10.1016/j.fm.2014.12.004 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA CD7UQ UT WOS:000351299900004 PM 25790987 ER PT J AU Oliver, AK Callaham, MA Jumpponen, A AF Oliver, Alena K. Callaham, Mac A., Jr. Jumpponen, Ari TI Soil fungal communities respond compositionally to recurring frequent prescribed burning in a managed southeastern US forest ecosystem SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire management; Pinus taeda; Prescribed fire; Soil fungi; Loblolly pine ID ALASKAN BOREAL FORESTS; PONDEROSA PINE FOREST; ECTOMYCORRHIZAL FUNGI; SCLEROPHYLL FOREST; RESISTANT PROPAGULES; MICROBIAL BIOMASS; HOST-SPECIFICITY; INTERIOR ALASKA; ORGANIC-MATTER; UNITED-STATES AB Prescribed fire is an important management tool to reduce fuel loads, to remove non-fire adapted species and to sustain fire-adapted taxa in many forested ecosystems of the southeastern USA. Yet, the long-term effects of recurring prescribed fires on soil fungi and their communities in these ecosystems remain unclear. We Illumina MiSeq sequenced and analyzed fungal Internal Transcribed Spacer (ITS2) amplicons from a long-term prescribed burn experiment that has implemented different regimes for nearly a quarter century to evaluate the effects of differing prescribed fire intervals and the season of their implementation on soil-inhabiting fungal communities. Unburned plots were used as a reference to represent the compositional state resulting from fire suppression. Our data show that while the recurring burning or the season of the prescribed burning do not affect richness and diversity of the fungal communities, the frequent (two and three year interval) fires maintain a fire-adapted community that is distinct from those in unburned reference plots. Subsequent indicator taxon analyses identified a total of 37 Operational Taxonomic Units (OTUs) that were more frequent in the frequently implemented burns and 26 OTUs that were more frequent in the fire suppressed treatment. We conclude that frequent prescribed burning maintains fire selected soil fungal communities that may support plant communities that are composed of desired fire adapted or fire tolerant species that dominate the frequently burned areas. (C) 2015 Elsevier B.V. All rights reserved. C1 [Oliver, Alena K.; Jumpponen, Ari] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Callaham, Mac A., Jr.] USDA, Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, Athens, GA 30602 USA. RP Jumpponen, A (reprint author), Kansas State Univ, Div Biol, 421 Ackert Hall, Manhattan, KS 66506 USA. FU United States Department of Agriculture Forest Service [11-CA-11330136-126] FX This work was supported by United States Department of Agriculture Forest Service Cooperative Agreement 11-CA-11330136-126 to A.J. We would like to thank Evelyn Wenk for coordinating the sampling and Alina Akhunova and Hanquan Xao for assistance during sequencing. Dr. Shawn Brown provided numerous insights and valuable suggestions on the sequence data analyses. The use of trade names in this contribution does not constitute official endorsement of these products by the United States Department of Agriculture or any of its subsidiary agencies. This is contribution no. 15-307-J from the Kansas Agricultural Experiment Station. NR 68 TC 7 Z9 8 U1 5 U2 103 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2015 VL 345 BP 1 EP 9 DI 10.1016/j.foreco.2015.02.020 PG 9 WC Forestry SC Forestry GA CE4OH UT WOS:000351809400001 ER PT J AU Peters, MP Iverson, LR Matthews, SN AF Peters, Matthew P. Iverson, Louis R. Matthews, Stephen N. TI Long-term droughtiness and drought tolerance of eastern US forests over five decades SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Cumulative drought severity index; Forest composition; Palmer Drought Severity Index; SDM ID CLIMATE-CHANGE; SEVERITY INDEX; UNITED-STATES; MORTALITY; TEMPERATURE; HABITAT; MODELS; TREES AB Droughts can influence forest composition directly by limiting water or indirectly by intensifying other stressors that affect establishment, growth, and mortality. Using community assemblages of eastern US tree species and drought tolerance characteristics assessed from literature, we examine recent drought conditions in relation to the spatial distribution of species and their tolerance to drought. First we calculate and compare a cumulative drought severity index (CDSI) for the conterminous US for the periods 1960-1986 and 1987-2013 using climate division Palmer Drought Severity Index (PDSI) values and a gridded self-calibrated PDSI dataset. This comparison indicates that drought conditions in the East tend to be less frequent and generally less severe than those in the West, and that the West has had a large increase in CDSI values in the latter period. Then we focus on the past and potential future role of droughtiness in eastern forests, which are relatively more diverse than western forests but have individual species that are uniquely affected by drought conditions. We found that eastern US forests tend to be relatively balanced in the composition of drought-tolerant and -intolerant species and that drought conditions are relatively uncommon in the East. Understanding the composition and distribution of drought tolerance levels within forests is crucial when managing for the impacts of drought (e.g., managing for survival), especially given the expected rise of drought in the future. (C) 2015 Published by Elsevier B.V. C1 [Peters, Matthew P.; Iverson, Louis R.; Matthews, Stephen N.] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. [Matthews, Stephen N.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. RP Peters, MP (reprint author), US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. EM matthewpeters@fs.fed.us RI Matthews, Stephen/D-1050-2012 NR 34 TC 9 Z9 9 U1 6 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 1 PY 2015 VL 345 BP 56 EP 64 DI 10.1016/j.foreco.2015.02.022 PG 9 WC Forestry SC Forestry GA CE4OH UT WOS:000351809400007 ER PT J AU Olanya, OM Niemira, BA Phillips, JG AF Olanya, Ocen M. Niemira, Brendan A. Phillips, John G. TI Effects of gamma irradiation on the survival of Pseudomonas fluorescens inoculated on romaine lettuce and baby spinach SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Irradiation sensitivity; Pseudomonas fluorescens survival; Doses; Leafy greens; Surface inoculations ID ESCHERICHIA-COLI O157-H7; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; RADIATION SENSITIVITY; MODIFIED ATMOSPHERE; FRESH PRODUCE; INACTIVATION; BIOCONTROL; PATHOGENS; BACTERIAL AB Irradiation of fresh fruits and vegetables has been utilized as a post-harvest intervention measure to inactivate microbial pathogen on produce. The objectives of this research were to determine the survival (D-10 values) of Pseudomonas fluorescens (Pf) strains 2-79, Q8R1, Q287, surface inoculated on baby spinach and romaine lettuce; and Pf strains suspended in buffer peptone water (BPW). Gamma irradiation doses of 0 (control), 0.25, 0.50, 0.75 and 1.0 kGy were applied on P. fluorescens inoculated on produce and stored for 24 h at 4 degrees C. On spinach and romaine lettuce, the D10 values of P. fluorescens ranged from 0.04 +/- 0.01 to 0.05 +/- 0.02 and from 0.05 +/- 0.03 to 0.06 +/- 0.03 kGy, respectively. Pf had high sensitivity to irradiation and its populations varied on spinach and on romaine lettuce. These results indicate that low irradiation values are required to inactivate Pf on produce resulting with low bacterial survival. Published by Elsevier Ltd. C1 [Olanya, Ocen M.; Niemira, Brendan A.] USDA ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Phillips, John G.] USDA, North Atlantic Area, Off Area, Wyndmoor, PA 19038 USA. RP Olanya, OM (reprint author), USDA ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov NR 26 TC 3 Z9 3 U1 6 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2015 VL 62 IS 1 BP 55 EP 61 DI 10.1016/j.lwt.2014.12.031 PN 1 PG 7 WC Food Science & Technology SC Food Science & Technology GA CE2KZ UT WOS:000351645800009 ER PT J AU Wang, LB Baldwin, EA Zhao, W Plotto, A Sun, XX Wang, Z Brecht, JK Bai, JH Yu, ZF AF Wang, Libin Baldwin, Elizabeth A. Zhao, Wei Plotto, Anne Sun, Xiuxiu Wang, Zhe Brecht, Jeffrey K. Bai, Jinhe Yu, Zhifang TI Suppression of volatile production in tomato fruit exposed to chilling temperature and alleviation of chilling injury by a pre-chilling heat treatment SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Solanum lycopersicum; Aroma; Ripening; Low temperature; Hot water ID FLAVOR VOLATILES; FRESH TOMATOES; STORAGE-TEMPERATURE; AROMA; QUALITY; PERCEPTION; GUAIACOL; WATER; BIOCHEMISTRY; DESCRIPTORS AB Chilling exposure of tomato fruit to 5 degrees C for less than 5 days at mature green stage does not cause visual symptom of chilling injury (Cl), however, it is unknown whether such conditions would impact flavour quality (internal Cl) after ripening, and if a pre-chilling heat treatment could alleviate internal Cl. In this experiment, mature green 'FL 47' tomatoes were gassed with ethylene to initiate ripening before heating and/or chilling treatment, and fruits were ripened at 20 degrees C after short exposure to the high or low temperature. Volatile analysis of the fruits were conducted after ripening. Chilling treatment generally suppressed production of aldehyde, alcohol, ketone, ester, acid and terpene volatile compounds, including the following abundant and/or important volatiles: hexanal, trans-2-hexenal, 6-methy1-5hepten-2-one, beta-ionone, 2-methylbutanal, 2-phenylethanol, guaiacol and 2-isobutylthiazole. Heat treatment alone did not affect most volatile levels after ripening. Heat treatment prior to chilling exposure alleviated the reduction of some key volatile compounds caused by chilling exposure, which agreed with sensory panel results in that panellists perceived more tomato flavour in "heating + chilling" treated fruit than fruit that were chilled only. Published by Elsevier Ltd. C1 [Wang, Libin; Yu, Zhifang] Nanjing Agr Univ, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Libin; Baldwin, Elizabeth A.; Zhao, Wei; Plotto, Anne; Sun, Xiuxiu; Wang, Zhe; Bai, Jinhe] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Brecht, Jeffrey K.] Univ Florida, IFAS, Dept Hort Sci, Gainesville, FL USA. RP Bai, JH (reprint author), USDA ARS, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov; yuzhifang@njau.edu.cn FU China Scholarship Council [201306850049]; Postgraduate Program in Jiangsu Province [CXLX13_267]; Big Red Tomato Packers, Fort Pierce, FL FX We wish to thank the financial support to the experiment from China Scholarship Council (201306850049), Postgraduate Program in Jiangsu Province (CXLX13_267) and the Big Red Tomato Packers, Fort Pierce, FL. NR 39 TC 8 Z9 8 U1 7 U2 60 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2015 VL 62 IS 1 BP 115 EP 121 DI 10.1016/j.lwt.2014.12.062 PN 1 PG 7 WC Food Science & Technology SC Food Science & Technology GA CE2KZ UT WOS:000351645800018 ER PT J AU Ahmed, OM Pangloli, P Hwang, CA Zivanovic, S Wu, T D'Souza, D Draughon, FA AF Ahmed, Omaima M. Pangloli, Philipus Hwang, Cheng-An Zivanovic, Svetlana Wu, Tao D'Souza, Doris Draughon, F. Ann TI The occurrence of Listeria monocytogenes in retail ready-to-eat meat and poultry products related to the levels of acetate and lactate in the products SO FOOD CONTROL LA English DT Article DE Listeria monocytogenes; Ready-to-eat; Meats; Lactate; Acetate ID LACTIC-ACID; UNITED-STATES; FOODS; FRESH; FILMS; STEAM; BEEF AB Listeria monocytogenes is a psychrotrophic foodborne pathogen that has been isolated from ready-to-eat meat and poultry products (RTE meats). The purpose of this study was to quantify lactate and acetate levels in retail RTE meats that had been tested in a previous study for the presence of L. monocytogenes to correlate the occurrence of L. monocytogenes to the acid levels. Products were extracted after blending 50 g of each sample with de-ionized water, and the extracts were quantified for lactate and acetate using HPLC. In general, the concentrations of both acids in samples varied with product types and manufacturers (p <0.05). The mean concentrations of lactate and acetate ranged from 10.71 to 23.03 mg/g (1.07-2.30%) and 0.66-1.56 mg/g (0.066-0.156%), respectively. The mean concentrations of lactate and acetate in L. monocytogenes-positive samples were 1.13-24.05 mg/g (0.11-2.4%) and 0-5.74 mg/g (0-0.574%), respectively. Results of this study indicate that RTE meats containing low levels of lactate were more likely to be positive for L monocytogenes while samples with higher concentrations of lactate and acetate were less likely to be positive for the pathogen. Therefore, the addition of lactate and acetate as antimicrobials is helpful as part of an overall Listeria control program. However, a rigorous sanitation and an effective HACCP program are also essential for control of Listeria. Published by Elsevier Ltd. C1 [Ahmed, Omaima M.; Pangloli, Philipus; Zivanovic, Svetlana; Draughon, F. Ann] Univ Tennessee, Dept Food Sci & Technol, Knoxville, TN 37996 USA. [Hwang, Cheng-An] USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wu, Tao] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China. RP Hwang, CA (reprint author), USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM andy.hwang@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service [1935-42000-057-11S]; National Alliance for Food Safety and Security; USDA/ARS FX This research was funded and provided by a grant from the United States Department of Agriculture-Agricultural Research Service, agreement no. 1935-42000-057-11S, and the National Alliance for Food Safety and Security. Appreciation is expressed to Drs. Elliot Ryser, Dean Cliver, and Omar Oryzabal for providing meat samples obtained in a USDA/ARS-funded project. NR 24 TC 8 Z9 9 U1 5 U2 35 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUN PY 2015 VL 52 BP 43 EP 48 DI 10.1016/j.foodcont.2014.12.015 PG 6 WC Food Science & Technology SC Food Science & Technology GA CC1CX UT WOS:000350079900007 ER PT J AU Liu, HQ Lu, LQ Pan, YJ Sun, XH Hwang, CA Zhao, Y Wu, VCH AF Liu, Haiquan Lu, Liqun Pan, Yingjie Sun, Xiaohong Hwang, Cheng-An Zhao, Yong Wu, Vivian C. H. TI Rapid detection and differentiation of Listeria monocytogenes and Listeria species in deli meats by a new multiplex PCR method SO FOOD CONTROL LA English DT Article DE Detection; Listeria monocytogenes; Listeria spp.; Multiplex PCR; Deli meat ID REAL-TIME PCR; RIBOSOMAL-RNA; GENUS LISTERIA; UNITED-STATES; SPP.; FOOD; IDENTIFICATION; VIRULENCE; DNA; STRAINS AB Listeria monocytogenes is an important foodborne pathogen. To effectively control this pathogen, it is necessary to have a method that can detect and differentiate L. monocytogenes from other Listeria species in food, environmental, and clinical samples. A new multiplex PCR method using new primers targeting the iap gene was developed to detect and differentiate L. monocytogenes and other Listeria species, and the method was applied to examine deli meat samples. Sixteen Listeria strains (8 L. monocytogenes, 2 Listeria innocua, 1 Listeria seeligeri, 3 Listeria welshimeri, 1 Listeria ivanovii, and 1 Listeria grayi) from the American Type Culture Collection were tested using the new PCR method in comparison to 16S rRNA sequencing assay and a previously described PCR method based on the detection of iap gene with other primers. The new PCR method was able to differentiate L. monocytogenes from the other Listeria species, while the 16S rRNA sequencing assay could not distinguish L. welshimeri from L. monocytogenes and the previously published multiplex PCR assay was not able to distinguish L. monocytogenes from L. innocua or L. ivanovii. Furthermore, 150 deli meat samples were analyzed by the newly developed multiplex PCR in comparison to ISO11290-1 method and 16S rRNA sequencing assay to detect L. monocytogenes and differentiate it from the other 5 species of Listeria. The developed PCR provided the same results as the ISO method. Out of 150 samples, 16 (10.7%) samples and 5 (33%) samples were tested positive and confirmed for Listeria spp. and L. monocytogenes, respectively. The multiplex PCR method is capable of discriminating between L. monogtogenes and other Listeria species. The method is simple and specific when combined with simplified enrichment procedures. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Liu, Haiquan; Pan, Yingjie; Sun, Xiaohong; Zhao, Yong] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China. [Liu, Haiquan; Lu, Liqun] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Natl Pathogen Collect Ctr Aquat Anim, Shanghai 201306, Peoples R China. [Liu, Haiquan; Pan, Yingjie; Sun, Xiaohong; Zhao, Yong] Shanghai Ocean Univ, Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Shanghai 201306, Peoples R China. [Hwang, Cheng-An] USDA ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wu, Vivian C. H.] Univ Maine, Sch Food & Agr, Orono, ME 04469 USA. RP Wu, VCH (reprint author), Univ Maine, Sch Food & Agr, 5735 Hitchner Hall, Orono, ME 04469 USA. EM yzhao@shou.edu.cn; vivian.wu@umitmaine.edu FU National Natural Science Foundation of China [31271870]; Science and Technology Commission of Shanghai Municipality [14DZ1205100, 14320502100, 12391901300]; Key Project of Shanghai Agriculture Prosperity through Science and Technology [3-5]; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation [11DZ2280300]; Ph.D. Programs Foundation of Ministry of Education of China [20103104110004]; Maine Agricultural and Forest Experiment Station at the University of Maine, USA [3398]; Hatch Grant from the USDA National Institute of Food and Agriculture [ME08562-10] FX This study was jointly supported by the National Natural Science Foundation of China (31271870), the project of Science and Technology Commission of Shanghai Municipality (14DZ1205100, 14320502100, 12391901300), Key Project of Shanghai Agriculture Prosperity through Science and Technology (2014, 3-5), Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation (11DZ2280300), the Ph.D. Programs Foundation of Ministry of Education of China (Project No. 20103104110004). This work was also supported by the Maine Agricultural and Forest Experiment Station at the University of Maine, USA, with external publication number 3398. This work is based upon research supported in part by Hatch Grant number ME08562-10 from the USDA National Institute of Food and Agriculture. The authors would like to thank Alison Lacombe (University of Maine) and the writing center of University of Maine for providing language help. NR 33 TC 6 Z9 6 U1 15 U2 58 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUN PY 2015 VL 52 BP 78 EP 84 DI 10.1016/j.foodcont.2014.12.017 PG 7 WC Food Science & Technology SC Food Science & Technology GA CC1CX UT WOS:000350079900012 ER PT J AU Smykal, P Coyne, CJ Ambrose, MJ Maxted, N Schaefer, H Blair, MW Berger, J Greene, SL Nelson, MN Besharat, N Vymyslicky, T Toker, C Saxena, RK Roorkiwal, M Pandey, MK Hu, JG Li, YH Wang, LX Guo, Y Qiu, LJ Redden, RJ Varshney, RK AF Smykal, Petr Coyne, Clarice J. Ambrose, Mike J. Maxted, Nigel Schaefer, Hanno Blair, Matthew W. Berger, Jens Greene, Stephanie L. Nelson, Matthew N. Besharat, Naghmeh Vymyslicky, Tomas Toker, Cengiz Saxena, Rachit K. Roorkiwal, Manish Pandey, Manish K. Hu, Jinguo Li, Ying H. Wang, Li X. Guo, Yong Qiu, Li J. Redden, Robert J. Varshney, Rajeev K. TI Legume Crops Phylogeny and Genetic Diversity for Science and Breeding SO CRITICAL REVIEWS IN PLANT SCIENCES LA English DT Review DE domestication; genetic diversity; legumes; Fabaceae; introgression; phylogeny; crop wild relatives ID CHICKPEA CICER-ARIETINUM; PISUM-SATIVUM L.; BEAN VICIA-FABA; PHASEOLUS-VULGARIS L.; NARROW-LEAFED LUPIN; BROOMRAPE OROBANCHE-CRENATA; LENS-CULINARIS MEDIK.; SOYBEAN GLYCINE-MAX; CHLOROPLAST-DNA PHYLOGENY; ALFALFA MEDICAGO-SATIVA AB Economically, legumes (Fabaceae) represent the second most important family of crop plants after the grass family, Poaceae. Grain legumes account for 27% of world crop production and provide 33% of the dietary protein consumed by humans, while pasture and forage legumes provide vital part of animal feed. Fabaceae, the third largest family of flowering plants, has traditionally been divided into the following three subfamilies: Caesalpinioideae, Mimosoideae, and Papilionoideae, all together with 800 genera and 20,000 species. The latter subfamily contains most of the major cultivated food and feed crops. Among the grain legumes are some of mankind's earliest crop plants, whose domestication parallelled that of cereals: Soybean in China; faba bean, lentil, chickpea and pea in the Fertile Crescent of the Near East; cowpeas and bambara groundnut in Africa; soybean and mungbeans in East Asia; pigeonpea and the grams in South Asia; and common bean, lima bean, scarlet runner bean, tepary bean and lupin in Central and South America. The importance of legumes is evidenced by their high representation in ex situ germplasm collections, with more than 1,000,000 accessions worldwide. A detailed knowledge of the phylogenetic relationships of the Fabaceae is essential for understanding the origin and diversification of this economically and ecologically important family of angiosperms. This review aims to combine the phylogenetic and genetic diversity approaches to better illustrate the origin, domestication history and preserved germplasm of major legume crops from 13 genera of six tribes and to indicate further potential both for science and agriculture. C1 [Smykal, Petr] Palacky Univ, Dept Bot, Olomouc 78371, Czech Republic. [Coyne, Clarice J.; Hu, Jinguo] WSU Pullman, USDA ARS, Pullman, WA 99164 USA. [Ambrose, Mike J.] John Innes Ctr, Norwich, Norfolk, England. [Maxted, Nigel] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England. [Schaefer, Hanno] Tech Univ Munich, Freising Weihenstephan, Germany. [Blair, Matthew W.] Tennessee State Univ, Dept Agr & Nat Sci, Nashville, TN 37203 USA. [Berger, Jens] CSIRO Plant Ind, Ctr Environm & Life Sci, Perth, WA, Australia. [Greene, Stephanie L.] USDA ARS, Prosser, WA 99350 USA. [Nelson, Matthew N.; Besharat, Naghmeh; Varshney, Rajeev K.] Univ Western Australia, Sch Plant Biol, Crawley, Australia. [Nelson, Matthew N.; Besharat, Naghmeh; Varshney, Rajeev K.] Univ Western Australia, UWA Inst Agr, Crawley, Australia. [Vymyslicky, Tomas] Agr Res Ltd, Troubsko, Czech Republic. [Toker, Cengiz] Akdeniz Univ, Dept Field Crops, TR-07058 Antalya, Turkey. [Saxena, Rachit K.; Roorkiwal, Manish; Pandey, Manish K.; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Li, Ying H.; Wang, Li X.; Guo, Yong; Qiu, Li J.] Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China. [Redden, Robert J.] Australian Grains Genebank, Horsham, Australia. RP Smykal, P (reprint author), Palacky Univ, Fac Sci, Dept Bot, Slechtitelu 11, Olomouc 78371, Czech Republic. EM petr.smykal@upol.cz RI Varshney, Rajeev/C-5295-2014; Nelson, Matthew/A-1421-2008; Toker, Cengiz/C-3681-2016; Berger, Jens/A-9768-2011 OI Varshney, Rajeev/0000-0002-4562-9131; Nelson, Matthew/0000-0001-6766-4117; FU Grant Agency of Palacky University [IGA PrF-2013-003]; Agricultural Research, Ltd.; MSM project [2629608001]; US Aid for International Development (USAID); Australian Indo Strategic Research Fund (AISRF) of Ministry of Science Technology; CoE Grant from the Department of Biotechnology, Government of India FX P.S. acknowledges fruitful discussions with numerous colleagues worldwide and financial support from the Grant Agency of Palacky University, IGA PrF-2013-003. T.M.'s work is funded as part of a long-term project by Agricultural Research, Ltd., and by MSM 2629608001 project. R.K.V. would like to thank US Aid for International Development (USAID), the Australian Indo Strategic Research Fund (AISRF) of Ministry of Science & Technology and the CoE Grant from the Department of Biotechnology, Government of India, for sponsoring research related to the topics discussed in this article at ICRISAT. NR 714 TC 29 Z9 30 U1 41 U2 740 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0735-2689 EI 1549-7836 J9 CRIT REV PLANT SCI JI Crit. Rev. Plant Sci. PD JUN 1 PY 2015 VL 34 IS 1-3 SI SI BP 43 EP 104 DI 10.1080/07352689.2014.897904 PG 62 WC Plant Sciences SC Plant Sciences GA AR5DK UT WOS:000343605200005 ER PT J AU Rubiales, D Fondevilla, S Chen, WD Gentzbittel, L Higgins, TJV Castillejo, MA Singh, KB Rispail, N AF Rubiales, Diego Fondevilla, Sara Chen, Weidong Gentzbittel, Laurent Higgins, Thomas J. V. Castillejo, Maria A. Singh, Karam B. Rispail, Nicolas TI Achievements and Challenges in Legume Breeding for Pest and Disease Resistance SO CRITICAL REVIEWS IN PLANT SCIENCES LA English DT Review DE marker assisted selection; insect; genetics; virus; parasitic weed; transcriptomics; fungus; disease resistance; proteomics; bacteria; metabolomics; oomycete; nematode ID PEA PISUM-SATIVUM; QUANTITATIVE TRAIT LOCI; CHICKPEA CICER-ARIETINUM; BROOMRAPE OROBANCHE-CRENATA; BEAN PHASEOLUS-VULGARIS; ALFALFA MEDICAGO-SATIVA; ROOT-KNOT NEMATODE; ARACHIS-HYPOGAEA L.; ASCOCHYTA BLIGHT RESISTANCE; POWDERY MILDEW RESISTANCE AB Yield stability of legume crops is constrained by a number of pest and diseases. Major diseases are rusts, powdery and downy mildews, ascochyta blights, botrytis gray molds, anthracnoses, damping-off, root rots, collar rots, vascular wilts and white molds. Parasitic weeds, viruses, bacteria, nematodes and damages caused by chewing and sap-sucking insects add to this long list of constraints for legume production. Their incidence and relative importance together with current understanding of their interactions with the host plants are presented. State of the art of current achievements and limitations for breeding for biotic stress resistance are listed and critically discussed. The recent development of large scale phenotyping, genome sequencing and analysis of gene, protein and metabolite expressions can be of great help to further decipher plant-pathogen interactions and identify key resistance components that may be introgressed into crop plants through breeding. C1 [Rubiales, Diego; Rispail, Nicolas] CSIC, Inst Sustainable Agr, Cordoba 14080, Spain. [Fondevilla, Sara] Goethe Univ Frankfurt, Inst Mol Biosci, D-60438 Frankfurt, Germany. [Chen, Weidong] Washington State Univ, Dept Plant Pathol, USDA ARS, Pullman, WA 99164 USA. [Gentzbittel, Laurent] Univ Toulouse, EcoLab, ENSAT, F-31326 Castanet Tolosan, France. [Higgins, Thomas J. V.] CSIRO Plant Ind, Canberra, ACT 2601, Australia. [Castillejo, Maria A.] Univ Vienna, Dept Mol Syst Biol, Vienna, Austria. [Singh, Karam B.] Univ Western Australia, CSIRO, Wembley, WA 6913, Australia. RP Rubiales, D (reprint author), CSIC, Inst Sustainable Agr, Apdo 4084, Cordoba 14080, Spain. EM diego.rubiales@ias.csic.es RI Rispail, Nicolas/I-4269-2014; Singh, Karam/C-3235-2012; OI Rispail, Nicolas/0000-0001-8730-0273; Singh, Karam/0000-0002-2777-7448; Castillejo, Maria Angeles/0000-0003-2017-1029; Fondevilla, Sara/0000-0003-0682-8135 NR 733 TC 17 Z9 17 U1 28 U2 545 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0735-2689 EI 1549-7836 J9 CRIT REV PLANT SCI JI Crit. Rev. Plant Sci. PD JUN 1 PY 2015 VL 34 IS 1-3 SI SI BP 195 EP 236 DI 10.1080/07352689.2014.898445 PG 42 WC Plant Sciences SC Plant Sciences GA AR5DK UT WOS:000343605200009 ER PT J AU Calero-Bernal, R Verma, SK Seaton, CT Sinnett, D Ball, E Dunams, D Rosenthal, BM Dubey, JP AF Calero-Bernal, Rafael Verma, Shiv K. Seaton, C. Tom Sinnett, David Ball, Erin Dunams, Detiger Rosenthal, Benjamin M. Dubey, Jitender P. TI Sarcocystis cruzi infection in wood bison (Bison bison athabascae) SO VETERINARY PARASITOLOGY LA English DT Article DE Conservation; PCR-RFLP; Protozoa; Sarcocystis cruzi; Wood bison; Bison bison athabascae ID BUFFALO BUBALUS-BUBALIS; INTERMEDIATE HOST; CATTLE; SEQUENCES; PROTOZOA AB Endangered wood bison (Bison bison athabascae) is the largest terrestrial mammal in the American continent. Animal health is an important issue in their conservation, and Sarcocystis cruzi may be a cause of clinical disease in Bovidae. Hearts of eight wood bison from Alaska, USA were examined for sarcocysts by histology, transmission electron microscopy, pepsin digestion, and molecularly. Sarcocystis bradyzoites were found in pepsin digests of all eight and sarcocysts were found in histologic sections of myocardium of four bison. Sarcocysts were thin-walled and ultrastructurally consistent with S. cruzi. Characterization of DNA obtained from lysis of pepsin liberated bradyzoites by PCR-RFLP and subsequent phylogenetic analyses matched with that previously reported for S. cruzi infecting cattle in the USA. Collectively, data indicate that wood bison is a natural intermediate host for S. cruzi. Published by Elsevier B.V. C1 [Calero-Bernal, Rafael; Verma, Shiv K.; Dunams, Detiger; Rosenthal, Benjamin M.; Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Seaton, C. Tom] Alaska Dept Fish & Game, Fairbanks, AK 99701 USA. [Sinnett, David] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Palmer, AK 99645 USA. [Ball, Erin] Joint Pathol Ctr, Vet Pathol Serv, Silver Spring, MD 20910 USA. RP Dubey, JP (reprint author), USDA, Anim Parasit Dis Lab, BARC, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov OI Rosenthal, Benjamin/0000-0002-0224-3773 FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund FX The authors thank Mr. Efrain Perez, Joint Pathology Center, Veterinary Services, U.S. Army, Silver Spring, Maryland for excellent technical help with electron microscopy. Rafael Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund. NR 20 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD MAY 30 PY 2015 VL 210 IS 1-2 BP 102 EP 105 DI 10.1016/j.vetpar.2015.03.007 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CI8UF UT WOS:000355046500014 PM 25868849 ER PT J AU Johnson, L Chen, TA Hughes, SO O'Connor, TM AF Johnson, Lauren Chen, Tzu-An Hughes, Sheryl O. O'Connor, Teresia M. TI The association of parent's outcome expectations for child TV viewing with parenting practices and child TV viewing: an examination using path analysis SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Television; Screen media; Parenting; Outcome expectations; Child ID PHYSICAL-ACTIVITY; SCREEN-TIME; SEXUAL-BEHAVIOR; TELEVISION EXPOSURE; FOOD ADVERTISEMENTS; NUTRITIONAL CONTENT; PRESCHOOL-CHILDREN; YOUNG-CHILDREN; UNITED-STATES; WATCHING SEX AB Background: Television (TV) viewing has been associated with many undesirable outcomes for children, such as increased risk of obesity, but TV viewing can also have benefits. Although restrictive parenting practices are effective in reducing children's TV viewing, not all parents use them and it is currently unclear why. The current study examined parenting practices related to TV viewing in the context of social-cognitive theory. Specifically, we hypothesized that positive and negative Parental Outcome Expectations for child's TV Viewing (POETV) would be associated with social co-viewing and restrictive parenting practices, and that POETV and parenting practices influence the amount of TV viewed by child. Method: Data were collected from an internet survey of 287 multi-ethnic parents and their 6-12 year old children on participants' sociodemographic information, parenting practices related to TV use, POETV, and parent and child TV viewing. Path analysis was used to examine the relationship amongst variables in separate models for weekday and weekend TV viewing. controlling for child age, household education, and parental TV viewing. Results: The results provided partial support for the hypotheses, with notable differences between weekday and weekend viewing. The models explained 13.6 % and 23.4 % of the variance in children's TV viewing on weekdays and weekends respectively. Neither positive nor negative POETV were associated with restrictive TV parenting in either model. One subscale each from positive and negative POETV were associated with social co-viewing parenting on both weekends and weekdays in the expected direction. Restrictive parenting practices were directly negatively associated with children's TV viewing on weekdays, but not weekends. Social co-viewing parenting was directly positively associated with children's TV viewing on weekends, but not weekdays. The strongest influence on children's TV viewing was having a TV in the child's bedroom. Negative POETV was weakly associated with having a TV in the child's room. Conclusions: These findings suggest that POETV and parenting may have a greater impact on weekend TV viewing, when children tend to watch more TV, than weekday. The models suggest that POETV, parenting and especially removing the TV from children's rooms may be promising targets for interventions. C1 [Johnson, Lauren] Baylor Coll Med, Menninger Dept Psychiat & Behav Sci, Houston, TX 77030 USA. [Johnson, Lauren] Childrens Hosp, New Orleans, LA USA. [Chen, Tzu-An; Hughes, Sheryl O.; O'Connor, Teresia M.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. [O'Connor, Teresia M.] Baylor Coll Med, Dept Pediat, Acad Gen Pediat, Houston, TX 77030 USA. RP O'Connor, TM (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. EM teresiao@bcm.edu FU Texas Children's Hospital Pilot Grant; US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM [USDA/ARS 6250-51000-053] FX This study was funded by a Texas Children's Hospital Pilot Grant. Texas Children's Hospital had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication. This work is a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM through a cooperative agreement (USDA/ARS 6250-51000-053). The contents of this publication do not necessarily reflect the views or policies of the USDA or Texas Children's Hospital, nor does mention of organizations imply endorsement from the US government. NR 55 TC 2 Z9 2 U1 6 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD MAY 28 PY 2015 VL 12 AR 70 DI 10.1186/s12966-015-0232-2 PG 9 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA CK1MH UT WOS:000355970900001 PM 26013560 ER PT J AU Koltes, JE Fritz-Waters, E Eisley, CJ Choi, I Bao, H Kommadath, A Serao, NVL Boddicker, NJ Abrams, SM Schroyen, M Loyd, H Tuggle, CK Plastow, GS Guan, LL Stothard, P Lunney, JK Liu, P Carpenter, S Rowland, RRR Dekkers, JCM Reecy, JM AF Koltes, James E. Fritz-Waters, Eric Eisley, Chris J. Choi, Igseo Bao, Hua Kommadath, Arun Serao, Nick V. L. Boddicker, Nicholas J. Abrams, Sam M. Schroyen, Martine Loyd, Hyelee Tuggle, Chris K. Plastow, Graham S. Guan, Leluo Stothard, Paul Lunney, Joan K. Liu, Peng Carpenter, Susan Rowland, Robert R. R. Dekkers, Jack C. M. Reecy, James M. TI Identification of a putative quantitative trait nucleotide in guanylate binding protein 5 for host response to PRRS virus infection SO BMC GENOMICS LA English DT Article ID RESPIRATORY SYNDROME VIRUS; DIFFERENTIAL EXPRESSION ANALYSIS; RNA-SEQ; ALIGNMENT; REPLICATION; PYROPTOSIS; DISCOVERY; OUTBREAK; DISEASE AB Background: Previously, we identified a major quantitative trait locus (QTL) for host response to Porcine Respiratory and Reproductive Syndrome virus (PRRSV) infection in high linkage disequilibrium (LD) with SNP rs80800372 on Sus scrofa chromosome 4 (SSC4). Results: Within this QTL, guanylate binding protein 5 (GBP5) was differentially expressed (DE) (p < 0.05) in blood from AA versus AB rs80800372 genotyped pigs at 7,11, and 14 days post PRRSV infection. All variants within the GBP5 transcript in LD with rs80800372 exhibited allele specific expression (ASE) in AB individuals (p < 0.0001). A transcript re-assembly revealed three alternatively spliced transcripts for GBP5. An intronic SNP in GBP5, rs340943904, introduces a splice acceptor site that inserts five nucleotides into the transcript. Individuals homozygous for the unfavorable AA genotype predominantly produced this transcript, with a shifted reading frame and early stop codon that truncates the 88 C-terminal amino acids of the protein. RNA-seq analysis confirmed this SNP was associated with differential splicing by QTL genotype (p < 0.0001) and this was validated by quantitative capillary electrophoresis (p < 0.0001). The wild-type transcript was expressed at a higher level in AB versus AA individuals, whereas the five-nucleotide insertion transcript was the dominant form in AA individuals. Splicing and ASE results are consistent with the observed dominant nature of the favorable QTL allele. The rs340943904 SNP was also 100 % concordant with rs80800372 in a validation population that possessed an alternate form of the favorable B QTL haplotype. Conclusions: GBP5 is known to play a role in inflammasome assembly during immune response. However, the role of GBP5 host genetic variation in viral immunity is novel. These findings demonstrate that rs340943904 is a strong candidate causal mutation for the SSC4 QTL that controls variation in host response to PRRSV. C1 [Koltes, James E.; Fritz-Waters, Eric; Eisley, Chris J.; Serao, Nick V. L.; Schroyen, Martine; Loyd, Hyelee; Tuggle, Chris K.; Carpenter, Susan; Dekkers, Jack C. M.; Reecy, James M.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Eisley, Chris J.; Liu, Peng] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Choi, Igseo; Abrams, Sam M.; Lunney, Joan K.] USDA ARS, BARC, APDL, Beltsville, MD 20705 USA. [Bao, Hua; Kommadath, Arun; Plastow, Graham S.; Guan, Leluo; Stothard, Paul] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Boddicker, Nicholas J.] Genesus Inc, Oakville, MB R0H 0Y0, Canada. [Rowland, Robert R. R.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. RP Reecy, JM (reprint author), Iowa State Univ, Dept Anim Sci, 2255 Kildee Hall, Ames, IA 50011 USA. EM jreecy@iastate.edu OI Plastow, Graham/0000-0002-3774-3110 FU USDA NIFA PRRS CAP [2008-55620-19132]; USDA NIFA [2012-38420-19286]; Genome Canada; Genome Alberta; Alberta Livestock and Meat Agency; PigGen Canada; National Pork Board; NRSP-8 Swine Genome and Bioinformatics Coordination projects; PHGC, Choice Genetics; PHGC, Fast Genetics; PHGC, Genesus; PHGC, PIC/Genus; PHGC, Topigs FX The authors acknowledge partial funding for this project provided by USDA NIFA PRRS CAP Award 2008-55620-19132, USDA NIFA award 2012-38420-19286, Genome Canada, Genome Alberta, the Alberta Livestock and Meat Agency and PigGen Canada. The samples for the RNA-seq analysis were provided by the PRRS Host Genetics Consortium (PHGC), which has been supported by the USDA NIFA PRRS CAP Award 2008-55620-19132, the National Pork Board, the NRSP-8 Swine Genome and Bioinformatics Coordination projects, and breeding companies associated with the PHGC, including Choice Genetics, Fast Genetics, Genesus, PIC/Genus, and Topigs. While members of the PRRS Host Genetics Consortium played important roles in the development of the original design for the PHGC studies, none of these funders had a role in the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication. NR 36 TC 10 Z9 10 U1 2 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 28 PY 2015 VL 16 AR 412 DI 10.1186/s12864-015-1635-9 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CJ0ED UT WOS:000355146100004 PM 26016888 ER PT J AU Huynh, MD Page, JT Richardson, BA Udall, JA AF Huynh, Mark D. Page, Justin T. Richardson, Bryce A. Udall, Joshua A. TI Insights into Transcriptomes of Big and Low Sagebrush SO PLOS ONE LA English DT Article ID SUBGENUS TRIDENTATAE; AGE DISTRIBUTIONS; EVOLUTION; ARTEMISIA; ASTERACEAE; DIVERSIFICATION; DUPLICATION; SELECTION AB We report the sequencing and assembly of three transcriptomes from Big (Artemisia tridentatassp. wyomingensis and Artemisia tridentatassp. tridentata) and Low (A. arbuscula ssp. arbuscula) sagebrush. The sequence reads are available in the Sequence Read Archive of NCBI. We demonstrate the utilities of these transcriptomes for gene discovery and phylogenomic analysis. An assembly of 61,883 transcripts followed by transcript identification by the program TRAPID revealed 16 transcripts directly related to terpene synthases, proteins critical to the production of multiple secondary metabolites in sagebrush. A putative terpene synthase was identified in two of our sagebrush samples. Using paralogs with synonymous mutations we reconstructed an evolutionary time line of ancient genome duplications. By applying a constant mutation rate to the data we estimate that these three ancient duplications occurred about 18, 34 and 60 million years ago. These transcriptomes offer a foundation for future studies of sagebrush, including inferences in chemical defense and the identification of species and subspecies of sagebrush for restoration and preservation of the threatened sage-grouse. C1 [Huynh, Mark D.; Page, Justin T.; Udall, Joshua A.] Brigham Young Univ, Plant & Wildlife Sci Dept, Provo, UT 84602 USA. [Richardson, Bryce A.] USDA, Forest Serv, Rocky Mt Res Stn, Provo, UT 84606 USA. RP Udall, JA (reprint author), Brigham Young Univ, Plant & Wildlife Sci Dept, Provo, UT 84602 USA. EM jaudall@byu.edu FU USDA Forest Service National Fire Plan; Great Basin Native Plant Program FX This study was supported by USDA Forest Service National Fire Plan, (http://www.forestsandrangelands.gov/resources/overview/nfp_research.sht ml), Great Basin Native Plant Program, (http://www.fs.fed.us/rm/boise/research/shrub/greatbasin.shtml). NR 40 TC 0 Z9 0 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 28 PY 2015 VL 10 IS 5 AR e0127593 DI 10.1371/journal.pone.0127593 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ0SC UT WOS:000355187300047 PM 26020526 ER PT J AU Thom, MD Daniels, JC Kobziar, LN Colburn, JR AF Thom, Matthew D. Daniels, Jaret C. Kobziar, Leda N. Colburn, Jonathan R. TI Can Butterflies Evade Fire? Pupa Location and Heat Tolerance in Fire Prone Habitats of Florida SO PLOS ONE LA English DT Article ID PRESCRIBED FIRE; PRAIRIE BUTTERFLIES; TALLGRASS PRAIRIE; PINUS-PALUSTRIS; MANAGEMENT; ABUNDANCE; FORESTS; CONSERVATION; HERPETOFAUNA; TEPHRITIDAE AB Butterflies such as the atala hairstreak, Eumaeus atala Poey, and the frosted elfin, Callophrys irus Godart, are restricted to frequently disturbed habitats where their larval host plants occur. Pupae of these butterflies are noted to reside at the base of host plants or in the leaf litter and soil, which may allow them to escape direct mortality by fire, a prominent disturbance in many areas they inhabit. The capacity of these species to cope with fire is a critical consideration for land management and conservation strategies in the locations where they are found. Survival of E. atala pupae in relation to temperature and duration of heat pulse was tested using controlled water bath experiments and a series of prescribed fire field experiments. Survival of E. atala pupae was correlated to peak temperature and heat exposure in both laboratory and field trials. In addition, E. atala survival following field trials was correlated to depth of burial; complete mortality was observed for pupae at the soil surface. Fifty percent of E. atala survived the heat generated by prescribed fire when experimentally placed at depths >= 1.75cm, suggesting that pupation of butterflies in the soil at depth can protect from fatal temperatures caused by fire. For a species such as E. atala that pupates above ground, a population reduction from a burn event is a significant loss, and so decreasing the impact of prescribed fire on populations is critical. C1 [Thom, Matthew D.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Daniels, Jaret C.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Daniels, Jaret C.; Colburn, Jonathan R.] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA. [Kobziar, Leda N.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. RP Thom, MD (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. EM matt.thom@ars.usda.gov FU UF Alumni Assistantship FX This paper was part of MDT's Ph.D. dissertation conducted at the University of Florida (UF) Entomology and Nematology Department, funded in part by a UF Alumni Assistantship. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 1 Z9 1 U1 7 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 27 PY 2015 VL 10 IS 5 AR e0126755 DI 10.1371/journal.pone.0126755 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ0RO UT WOS:000355185600059 PM 26016779 ER PT J AU Brar, S Tsui, CKM Dhillon, B Bergeron, MJ Joly, DL Zambino, PJ El-Kassaby, YA Hamelin, RC AF Brar, Simren Tsui, Clement K. M. Dhillon, Braham Bergeron, Marie-Josee Joly, David L. Zambino, P. J. El-Kassaby, Yousry A. Hamelin, Richard C. TI Colonization History, Host Distribution, Anthropogenic Influence and Landscape Features Shape Populations of White Pine Blister Rust, an Invasive Alien Tree Pathogen SO PLOS ONE LA English DT Article ID CRONARTIUM-RIBICOLA; NORTH-AMERICA; BIOLOGICAL INVASIONS; AERIAL DISPERSAL; PLANT-DISEASE; LIMBER PINE; 1ST REPORT; GENETICS; EPIDEMIOLOGY; RESISTANCE AB White pine blister rust is caused by the fungal pathogen Cronartium ribicola J.C. Fisch (Basidiomycota, Pucciniales). This invasive alien pathogen was introduced into North America at the beginning of the 20th century on pine seedlings imported from Europe and has caused serious economic and ecological impacts. In this study, we applied a population and landscape genetics approach to understand the patterns of introduction and colonization as well as population structure and migration of C. ribicola. We characterized 1,292 samples of C. ribicola from 66 geographic locations in North America using single nucleotide polymorphisms (SNPs) and evaluated the effect of landscape features, host distribution, and colonization history on the structure of these pathogen populations. We identified eastern and western genetic populations in North America that are strongly differentiated. Genetic diversity is two to five times higher in eastern populations than in western ones, which can be explained by the repeated accidental introductions of the pathogen into northeastern North America compared with a single documented introduction into western North America. These distinct genetic populations are maintained by a barrier to gene flow that corresponds to a region where host connectivity is interrupted. Furthermore, additional cryptic spatial differentiation was identified in western populations. This differentiation corresponds to landscape features, such as mountain ranges, and also to host connectivity. We also detected genetic differentiation between the pathogen populations in natural stands and plantations, an indication that anthropogenic movement of this pathogen still takes place. These results highlight the importance of monitoring this invasive alien tree pathogen to prevent admixture of eastern and western populations where different pathogen races occur. C1 [Brar, Simren; Tsui, Clement K. M.; Dhillon, Braham; El-Kassaby, Yousry A.; Hamelin, Richard C.] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V5Z 1M9, Canada. [Bergeron, Marie-Josee; Hamelin, Richard C.] Nat Resources Canada, Laurentian Forestry Ctr, Quebec City, PQ, Canada. [Joly, David L.] Univ Moncton, Dept Biol, Moncton, NB E1A 3E9, Canada. [Zambino, P. J.] US Forest Serv, USDA, Coeur dAlene Field Off, Coeur Dalene, ID USA. RP Hamelin, RC (reprint author), Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V5Z 1M9, Canada. EM Richard.hamelin@ubc.ca RI El-Kassaby, Yousry/K-9856-2016; Tsui, Kin-Ming (Clement)/D-3763-2011; Tsui, KM/F-6416-2013 OI El-Kassaby, Yousry/0000-0002-4887-8977; Tsui, KM/0000-0001-5129-1037 FU NSERC; Genome Canada FX This study was funded by an NSERC Discovery grant to RCH that supported a research assistant-ship to SB, and by a Genome Canada Large Scale Applied Genomics grant to RCH that supported BD and CKMT, (http://www.genomecanada.ca/en/portfolio/research/2010-competition.aspx) . NR 56 TC 1 Z9 1 U1 11 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 26 PY 2015 VL 10 IS 5 AR e0127916 DI 10.1371/journal.pone.0127916 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ0QZ UT WOS:000355183900165 PM 26010250 ER PT J AU Baillie, BR Neary, DG AF Baillie, Brenda R. Neary, Daniel G. TI Water quality in New Zealand's planted forests: a review SO NEW ZEALAND JOURNAL OF FORESTRY SCIENCE LA English DT Review DE New Zealand; Planted forest; Water quality; Land use ID HILL-COUNTRY STREAMS; LAND-USE INFLUENCES; PUMICE-BED STREAMS; BENTHIC INVERTEBRATES; RIPARIAN BUFFERS; PINE PLANTATION; MOTUEKA RIVER; NORTH-ISLAND; MACROINVERTEBRATE COMMUNITIES; SOUTH ISLAND AB This paper reviewed the key physical, chemical and biological water quality attributes of surface waters in New Zealand's planted forests. The purpose was to: a) assess the changes in water quality throughout the planted forestry cycle from afforestation through to harvesting; b) compare water quality from planted forests with other land uses in New Zealand; and c) identify knowledge gaps and opportunities for future research. Afforestation of pasture land significantly improved a wide range of water quality attributes such as stream temperature, nutrient and sediment concentrations and microbial contamination within 4-6 years of planting. Water quality in mid-rotation to mature forests, a large proportion of the forestry cycle, was highly variable but characterised by cool water temperatures, low concentrations of sediment and nutrients, with aquatic invertebrate communities indicative of high water quality. Impacts of timber harvesting on water quality attributes were greatest when clear-cut harvesting up to the stream edge. Harvest impacts were mediated by the retention of intact riparian buffers and to a lesser extent by retention of moderate quantities of logging slash across small stream channels. Temporal and spatial factors influenced the magnitude of response to harvesting activities and duration of the recovery period. Land-use comparisons generally showed improving water quality from pasture to planted forest to indigenous forest. Continued research to identify management systems that mitigate impacts on water quality, particularly during harvesting, remains a priority. Consistent approaches to water quality monitoring will improve the ability to report on water quality in planted forests. Future water quality research in New Zealand's planted forests needs to encompass emerging contaminants of national and international concern such as pesticides and pharmaceuticals. As New Zealand moves toward increasing productivity from planted forests, use of potential interventions such as intensified herbicide and fertiliser use, will need to be within sustainable freshwater limits. A future challenge is to quantify the economic value of freshwater ecosystems services provided by New Zealand's planted forests. This review showed that with continued prudent stewardship and ongoing improvements in management practices, particularly during harvesting, New Zealand's planted forests can provide a sustainable source of well-maintained and high quality water resources. C1 [Baillie, Brenda R.] SCION, Rotorua 3046, New Zealand. [Neary, Daniel G.] USDA Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Baillie, BR (reprint author), SCION, Private Bag 3020, Rotorua 3046, New Zealand. EM brenda.baillie@scionresearch.com FU Ministry of Business, Innovation and Employment (MBIE) 'Protecting and enhancing the environment through forestry programme [C04X0806] FX This work was funded by the Ministry of Business, Innovation and Employment (MBIE) 'Protecting and enhancing the environment through forestry programme C04X0806. We would like to thank various authors of papers in this review for assisting with enquiries and information requests. Russell Death and Keith Moser reviewed earlier versions of the manuscript. Duncan Harrison compiled the data on stream kilometres in New Zealand's planted forests. NR 117 TC 4 Z9 7 U1 9 U2 64 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0048-0134 EI 1179-5395 J9 NZ J FORESTRY SCI JI N. Z. J. Forest. Sci. PD MAY 22 PY 2015 VL 45 AR 7 DI 10.1186/s40490-015-0040-0 PG 18 WC Forestry SC Forestry GA CQ6MH UT WOS:000360718000001 ER PT J AU Pacheco, JM Smoliga, GR O'Donnell, V Brito, BP Stenfeldt, C Rodriguez, LL Arzt, J AF Pacheco, Juan M. Smoliga, George R. O'Donnell, Vivian Brito, Barbara P. Stenfeldt, Carolina Rodriguez, Luis L. Arzt, Jonathan TI Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression SO PLOS ONE LA English DT Article ID HEPATITIS-C VIRUS; IMMUNE-RESPONSES; CARRIER STATE; ALPHA/BETA INTERFERON; GAMMA-INTERFERON; EPITHELIAL-CELLS; BOVINE-TISSUES; RNA; INNATE; PATHOGENESIS AB Tissues obtained post-mortem from cattle persistently infected with foot-and-mouth disease virus (FMDV) were analyzed to characterize the tissue-specific localization of FMDV and partial transcriptome profiles for selected immunoregulatory cytokines. Analysis of 28 distinct an-atomic sites from 21 steers infected with FMDV serotype A, O or SAT2, had the highest prevalence of overall viral detection in the dorsal nasopharynx (80.95%) and dorsal soft palate (71.43%). FMDV was less frequently detected in laryngeal mucosal tissues, oropharyngeal mucosal sites, and lymph nodes draining the pharynx. Immunomicroscopy indicated that within persistently infected mucosal tissues, FMDV antigens were rarely detectable within few epithelial cells in regions of mucosa-associated lymphoid tissue (MALT). Transcriptome analysis of persistently infected pharyngeal tissues by qRT-PCR for 14 cytokine genes indicated a general trend of decreased mRNA levels compared to uninfected control animals. Although, statistically significant differences were not observed, greatest suppression of relative expression (RE) was identified for IP-10 (RE = 0.198), IFN-beta (RE = 0.269), IL-12 (RE = 0.275), and IL-2 (RE = 0.312). Increased relative expression was detected for IL-6 (RE = 2.065). Overall, this data demonstrates that during the FMDV carrier state in cattle, viral persistence is associated with epithelial cells of the nasopharynx in the upper respiratory tract and decreased levels of mRNA for several immunoregulatory cytokines in the infected tissues. C1 [Pacheco, Juan M.; Smoliga, George R.; O'Donnell, Vivian; Stenfeldt, Carolina; Rodriguez, Luis L.; Arzt, Jonathan] USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Plum Isl, NY USA. [O'Donnell, Vivian] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Brito, Barbara P.] Univ Calif Davis, Ctr Anim Dis Modeling & Surveillance, Davis, CA 95616 USA. RP Arzt, J (reprint author), USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Plum Isl, NY USA. EM jonathan.arzt@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201; Stenfeldt, Carolina/0000-0002-2074-3886; Arzt, Jonathan/0000-0002-7517-7893 FU USDA-ARS CRIS [8064-32000-057-00D]; Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQPM-13-X-00131]; Plum Island Animal Disease Center Research Participation Program fellowship FX This project was funded by USDA-ARS CRIS project 8064-32000-057-00D and by the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQPM-13-X-00131. CS is a recipient of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an inter-agency agreement with the U.S. Department of Energy. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 71 TC 10 Z9 11 U1 2 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 21 PY 2015 VL 10 IS 5 AR e0125698 DI 10.1371/journal.pone.0125698 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CK7VW UT WOS:000356444000012 PM 25996935 ER PT J AU Elston, R Friedman, C Gustafson, L Meyer, G Rogers, R AF Elston, Ralph Friedman, Carolyn Gustafson, Lori Meyer, Gary Rogers, Russell TI Denman Island disease in Washington State, USA: distribution and prevalence in Pacific and Olympia oysters SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Denman Island disease; Mikrocytos mackini; Crassostrea gigas; Ostrea lurida ID CRASSOSTREA-GIGAS THUNBERG; MIKROCYTOS-MACKINI; CAUSATIVE AGENT; NOCARDIOSIS; RESISTANCE; PARASITES AB We sampled over 2400 wild, feral, and cultured Pacific oysters Crassostrea gigas and Olympia oysters Ostrea lurida in Washington State, USA, from 2002 to 2006 to estimate the prevalence of infection with Mikrocytos mackini, the causative agent of Denman Island disease. Both histology and qualitative PCR methods were used. Estimates of true prevalence of M. mackini in fection in C. gigas, after accounting for imperfect test sensitivity, ranged from mean values of 0 to 10.0% by histology and 0 to 8.4% based on pooled PCR samples. M. mackini was not detected in any of the O. lurida samples. Results suggest a lower prevalence of the pathogen and severity of this oyster disease in Washington than that indicated in previous reports from British Columbia, Canada, potentially attributable to higher seawater temperatures in the Washington sample locations. C1 [Elston, Ralph] AquaTechnics, Sequim, WA 98382 USA. [Friedman, Carolyn] Univ Washington, Sch Aquat & Fisheries Sci, Seattle, WA 98105 USA. [Gustafson, Lori] USDA APHIS Vet Serv, Ft Collins, CO 80526 USA. [Meyer, Gary] Fisheries & Oceans Canada, Pacific Biol Stn, Nanaimo, BC V9T 6N7, Canada. [Rogers, Russell] Washington Dept Fish & Wildlife, Olympia, WA 98501 USA. RP Elston, R (reprint author), AquaTechnics, 455 W Bell St, Sequim, WA 98382 USA. EM ralph@aquatechnics.com FU Shellfish Disease, Pest, and Predator Program of the Washington Department of Fish and Wildlife, Olympia, Washington FX Funding for the study (with the exception of the initial 2002 sample) was provided by the Shellfish Disease, Pest, and Predator Program of the Washington Department of Fish and Wildlife, Olympia, Washington. NR 21 TC 2 Z9 2 U1 1 U2 9 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD MAY 21 PY 2015 VL 114 IS 2 BP 147 EP 154 DI 10.3354/dao02862 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA CJ0FE UT WOS:000355150100006 PM 25993889 ER PT J AU Zanne, AE Tank, DC Cornwell, WK Eastman, JM Smith, SA FitzJohn, RG McGlinn, DJ O'Meara, BC Moles, AT Reich, PB Royer, DL Soltis, DE Stevens, PF Westoby, M Wright, IJ Aarssen, L Bertin, RI Calaminus, A Govaerts, R Hemmings, F Leishman, MR Oleksyn, J Soltis, PS Swenson, NG Warman, L Beaulieu, JM AF Zanne, Amy E. Tank, David C. Cornwell, William K. Eastman, Jonathan M. Smith, Stephen A. FitzJohn, Richard G. McGlinn, Daniel J. O'Meara, Brian C. Moles, Angela T. Reich, Peter B. Royer, Dana L. Soltis, Douglas E. Stevens, Peter F. Westoby, Mark Wright, Ian J. Aarssen, Lonnie Bertin, Robert I. Calaminus, Andre Govaerts, Rafael Hemmings, Frank Leishman, Michelle R. Oleksyn, Jacek Soltis, Pamela S. Swenson, Nathan G. Warman, Laura Beaulieu, Jeremy M. TI Doubtful pathways to cold tolerance in plants reply SO NATURE LA English DT Letter ID EVOLUTION C1 [Zanne, Amy E.] George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA. [Zanne, Amy E.] Missouri Bot Garden, Ctr Conservat & Sustainable Dev, St Louis, MO 63121 USA. [Tank, David C.; Eastman, Jonathan M.] Univ Idaho, Dept Biol Sci, Moscow, ID 83844 USA. [Tank, David C.; Eastman, Jonathan M.] Univ Idaho, Inst Bioinformat & Evolutionary Studies, Moscow, ID 83844 USA. [Cornwell, William K.] Dept Ecol Sci, Syst Ecol, NL-1081 HV Amsterdam, Netherlands. [Cornwell, William K.; Moles, Angela T.; Hemmings, Frank; Warman, Laura] Univ New S Wales, Sch Biol Earth & Environm Sci, Evolut & Ecol Res Ctr, Sydney, NSW 2052, Australia. [Smith, Stephen A.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [FitzJohn, Richard G.] Univ British Columbia, Dept Zool, Vancouver, BC V6T 1Z4, Canada. [FitzJohn, Richard G.] Univ British Columbia, Biodivers Res Ctr, Vancouver, BC V6T 1Z4, Canada. [FitzJohn, Richard G.; Westoby, Mark; Wright, Ian J.; Leishman, Michelle R.] Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia. [McGlinn, Daniel J.] Coll Charleston, Dept Biol, Charleston, SC 29424 USA. [O'Meara, Brian C.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN 37996 USA. [Reich, Peter B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Royer, Dana L.] Wesleyan Univ, Dept Earth & Environm Sci, Middletown, CT 06459 USA. [Soltis, Douglas E.; Calaminus, Andre] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Soltis, Douglas E.; Soltis, Pamela S.] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA. [Soltis, Douglas E.; Soltis, Pamela S.] Univ Florida, Genet Inst, Gainesville, FL 32611 USA. [Stevens, Peter F.] Univ Missouri, Dept Biol, St Louis, MO 63121 USA. [Aarssen, Lonnie] Queens Univ, Dept Biol, Kingston, ON K7L 3N6, Canada. [Bertin, Robert I.] Coll Holy Cross, Dept Biol, Worcester, MA 01610 USA. [Govaerts, Rafael] Royal Bot Gardens, Richmond TW9 3AB, Surrey, England. [Oleksyn, Jacek] Polish Acad Sci, Inst Dendrol, PL-62035 Kornik, Poland. [Swenson, Nathan G.] Michigan State Univ, Dept Plant Biol & Ecol, Evolutionary Biol & Behav, Program, E Lansing, MI 48824 USA. [Warman, Laura] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Beaulieu, Jeremy M.] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. RP Zanne, AE (reprint author), George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA. EM aezanne@gmail.com RI Leishman, Michelle/G-9726-2012; Wright, Ian/G-4979-2012; Soltis, Pamela/L-5184-2015; Warman, Laura/C-6651-2011; Moles, Angela/C-3083-2008 OI Leishman, Michelle/0000-0003-4830-5797; Wright, Ian/0000-0001-8338-9143; Warman, Laura/0000-0001-7339-749X; Moles, Angela/0000-0003-2041-7762 NR 10 TC 0 Z9 1 U1 4 U2 42 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD MAY 21 PY 2015 VL 521 IS 7552 BP E6 EP E7 DI 10.1038/nature14394 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI5RM UT WOS:000354816500016 PM 25993971 ER PT J AU Zhang, FM Li, XR Zhang, YH Coates, B Zhou, XG Cheng, DF AF Zhang, Fangmei Li, Xiangrui Zhang, Yunhui Coates, Brad Zhou, Xuguo Joe Cheng, Dengfa TI Bacterial symbionts, Buchnera, and starvation on wing dimorphism in English grain aphid, Sitobion avenae (F.) (Homoptera: Aphididae) SO FRONTIERS IN PHYSIOLOGY LA English DT Article DE Sitobion avenae; Buchnera; wing dimorphism; starvation; obligate symbiont; nutrition; biotic factors; nutritional requirements ID APOSYMBIOTIC PEA APHIDS; AMINO-ACID-COMPOSITION; ACYRTHOSIPHON-PISUM; MYZUS-PERSICAE; MYCETOCYTE SYMBIOSIS; ENDOSYMBIOTIC BACTERIA; DIET COMPOSITION; MEGOURA-VICIAE; PHLOEM SAP; PLANT AB Wing dimorphism in aphids can be affected by multiple cues, including both biotic (nutrition, crowding, interspecific interactions, the presence of natural enemies, maternal and transgenerational effects, and alarm pheromone) and abiotic factors (temperature, humidity, and photoperiod). The majority of the phloem-feeding aphids carry Buchnera, an obligate symbiotic proteobacteria. Buchnera has a highly reduced genome size, but encode key enzymes in the tryptophan biosynthetic pathway and is crucial for nutritional balance, development and reproduction in aphids. In this study, we investigated the impact of two nutritional-based biotic factors, symbionts and starvation, on the wing dimorphism in the English grain aphid, Sitobion avenae, a devastating insect pest of cereal crops (e.g., wheat) worldwide. Elimination of Buchnera using the antibiotic rifampicin significantly reduced the formation of winged morphs, body mass, and fecundity in S. avenae. Furthermore, the absence of this primary endosymbiont may disrupt the nutrient acquisition in aphids and alter transgenerational phenotypic expression. Similarly, both survival rate and the formation of winged morphs were substantially reduced after neonatal (<24 h old) offspring were starved for a period of time. The combined results shed light on the impact of two nutritional-based biotic factors on the phenotypic plasticity in aphids. A better understanding of the wing dimorphism in aphids will provide the theoretical basis for the prediction and integrated management of these phloem-feeding insect pests. C1 [Zhang, Fangmei; Li, Xiangrui; Zhang, Yunhui; Cheng, Dengfa] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Coates, Brad] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA USA. [Zhou, Xuguo Joe] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. RP Zhou, XG (reprint author), Univ Kentucky, Dept Entomol, S-225 Agr Sci Ctr North, Lexington, KY 40546 USA. EM xuguozhou@uky.edu; dfcheng@ippcaas.cn FU National Natural Science Foundation of China [31301659]; National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China [2012BAD19B04]; China Agriculture Research System [CARS-3]; Kentucky Agricultural Experiment Station [15-08-048] FX The authors are grateful to anonymous reviewers and editor for their constructive criticisms. Special thanks go to Dr. Runzhi Li (Shanxi Agricultural University) for his comments on an earlier draft. This research was supported by the National Natural Science Foundation of China (Award Number: 31301659), National "Twelfth Five-Year" Plan for Science & Technology Support Development Program of China (Award Number: 2012BAD19B04), China Agriculture Research System (Award Number: CARS-3). The information reported in this paper (No. 15-08-048) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. The granting agencies have no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 61 TC 2 Z9 2 U1 5 U2 26 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-042X J9 FRONT PHYSIOL JI Front. Physiol. PD MAY 20 PY 2015 VL 6 AR 155 DI 10.3389/fphys.2015.00155 PG 9 WC Physiology SC Physiology GA CM3OG UT WOS:000357591200001 PM 26042046 ER PT J AU Tschann, JM Martinez, SM Penilla, C Gregorich, SE Pasch, LA de Groat, CL Flores, E Deardorff, J Greenspan, LC Butte, NF AF Tschann, Jeanne M. Martinez, Suzanna M. Penilla, Carlos Gregorich, Steven E. Pasch, Lauri A. de Groat, Cynthia L. Flores, Elena Deardorff, Julianna Greenspan, Louise C. Butte, Nancy F. TI Parental feeding practices and child weight status in Mexican American families: a longitudinal analysis SO INTERNATIONAL JOURNAL OF BEHAVIORAL NUTRITION AND PHYSICAL ACTIVITY LA English DT Article DE Feeding practices; Mexican Americans; Latinos; Child; Bodymass index; Obesity; Parents; Fathers; Longitudinal ID BODY-MASS INDEX; PRESCHOOL-AGED CHILDREN; WAIST CIRCUMFERENCE; EATING BEHAVIORS; OVERWEIGHT CHILDREN; MATERNAL CONCERN; RISK-FACTORS; OBESITY; ADIPOSITY; MOTHERS AB Background: Parental feeding practices are thought to influence children's weight status, through children's eating behavior and nutritional intake. However, because most studies have been cross-sectional, the direction of influence is unclear. Moreover, although obesity rates are high among Latino children, few studies of parental feeding practices have focused on this population. Methods: This 2-year longitudinal study examined mutual influences over time between parental feeding practices and children's weight status, in Mexican American families with children 18 years old at baseline. Mothers (n = 322) and fathers (n = 182) reported on their feeding practices at baseline, 1-year follow-up, and 2-year follow-up. Weight status, defined by waist-height ratio (WHtR) and body mass index (BMI), was ascertained at all assessments. Cross-lagged panel models were used to examine the mutual influences of parental feeding practices and child weight status over time, controlling for covariates. Results: Both mothers' and fathers' restriction of food predicted higher subsequent child weight status at Year 1, and for fathers this effect was also found at Year 2. Mothers' and fathers' pressure to eat predicted lower weight status among boys, but not girls, at Year 1. Child weight status also predicted some parental feeding practices: boys' heavier weight predicted mothers' less pressure to eat at Year 1, less use of food to control behavior at Year 2, and greater restriction at Year 2; and girls' heavier weight at Year 1 predicted fathers' less pressure to eat and less positive involvement in child eating at Year 2. Conclusions: This study provides longitudinal evidence that some parental feeding practices influence Mexican American children's weight status, and that children's weight status also influences some parental feeding practices. Feeding practices of both mothers and fathers were related to children's weight status, underscoring the importance of including fathers in research on parental feeding practices and child obesity. C1 [Tschann, Jeanne M.; Pasch, Lauri A.; de Groat, Cynthia L.] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. [Martinez, Suzanna M.] Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA. [Penilla, Carlos] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA. [Gregorich, Steven E.] Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA. [Flores, Elena] Univ San Francisco, Sch Educ, Counseling Psychol Dept, San Francisco, CA 94118 USA. [Deardorff, Julianna] Univ Calif Berkeley, Sch Publ Hlth, Div Community Hlth & Human Dev, Berkeley, CA 94720 USA. [Greenspan, Louise C.] Kaiser Permanente, San Francisco, CA 94115 USA. [Butte, Nancy F.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Tschann, JM (reprint author), Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. EM tschannj@healthpsych.ucsf.edu FU National Heart, Lung and Blood Institute [R01 HL084404] FX This research was support by grant R01 HL084404 from the National Heart, Lung and Blood Institute awarded to J.M. Tschann. We thank Jennifer Cho, Irene Takahashi, and the Kaiser Foundation Research Institute, which provided access to members of Kaiser. NR 60 TC 1 Z9 1 U1 7 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1479-5868 J9 INT J BEHAV NUTR PHY JI Int. J. Behav. Nutr. Phys. Act. PD MAY 20 PY 2015 VL 12 AR 66 DI 10.1186/s12966-015-0224-2 PG 10 WC Nutrition & Dietetics; Physiology SC Nutrition & Dietetics; Physiology GA CJ6BY UT WOS:000355579300001 PM 25986057 ER PT J AU Smith, DJ Shelver, WL Baynes, RE Tell, L Gehring, R Li, MJ Dutko, T Schroeder, JW Herges, G Riviere, JE AF Smith, David J. Shelver, Weilin L. Baynes, Ronald E. Tell, Lisa Gehring, Ronette Li, Mengjie Dutko, Terry Schroeder, J. W. Herges, Grant Riviere, Jim E. TI Excretory, Secretory, and Tissue Residues after Label and Extra-label Administration of Flunixin Meglumine to Saline- or Lipopolysaccharide-Exposed Dairy Cows SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE dairy; extra-label; flunixin; holstein; liver; metabolite ratio; milk; residue ID DRUG-METABOLIZING-ENZYMES; HEPATIC CYTOCHROME-P450; BOVINE-MILK; PHARMACOKINETICS; INFLAMMATION; CATTLE; PLASMA; TRANSPORTERS; EXPRESSION; MASTITIS AB Twenty lactating dairy cattle were intravenously infused with either lipopolysaccharide (LPS) (n = 10) or sterile saline (n = 10). Five cattle in each group received three doses of flunixin meglumine administered by either intravenous infusion or intramuscular injection at 24 h intervals. Milk, urine, and tissues were collected. Thirty-six hours after the last flunixin administration, milk from six cows contained 5-hydroxyflunixin (5OHF) levels greater than the milk tolerance of 2 ng/mL; by 48 h, milk from two cows, a saline and a LPS-treated animal, had violative milk concentrations of 5OHF. A single animal treated with LPS and intramuscular flunixin contained violative flunixin residues in liver. The ratio of urinary flunixin/5OHF was correlated (P < 0.01; R-2 = 0.946) with liver flunixin residues in LPS-treated animals, but not (P = 0.96; R-2 = 0.003) in cows treated with saline in lieu of LPS. Violative residues of flunixin in dairy cattle may be related to LPS inhibition of flunixin metabolism. C1 [Smith, David J.; Shelver, Weilin L.; Herges, Grant] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. [Baynes, Ronald E.] N Carolina State Univ, Coll Vet Med, Raleigh, NC 27607 USA. [Tell, Lisa] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA. [Gehring, Ronette; Li, Mengjie; Riviere, Jim E.] Kansas State Univ, Inst Computat Comparat Med, Coll Vet Med, Manhattan, KS 66506 USA. [Dutko, Terry] USDA FSIS, Off Publ Hlth Sci, Midwestern Lab, St Louis, MO 63120 USA. [Schroeder, J. W.] N Dakota State Univ, Dept Anim Sci, Fargo, ND 58108 USA. RP Smith, DJ (reprint author), USDA ARS, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM david.j.smith@ars.usda.gov FU USDA-NIFA FX Support for the Food Animal Residue Avoidance Databank program (FARAD) at KSU, UC-Davis, and NCSU was provided by USDA-NIFA. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture, the Agricultural Research Service, or the Food Safety and Inspection Service of any product or service to the exclusion of others that may be suitable. NR 35 TC 4 Z9 4 U1 0 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 20 PY 2015 VL 63 IS 19 BP 4893 EP 4901 DI 10.1021/acs.jafc.5b01509 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CJ0GS UT WOS:000355154400024 PM 25950946 ER PT J AU Downs, CJ Stewart, KM Dick, BL AF Downs, Cynthia J. Stewart, Kelley M. Dick, Brian L. TI Investment in Constitutive Immune Function by North American Elk Experimentally Maintained at Two Different Population Densities SO PLOS ONE LA English DT Article ID LIFE-HISTORY STRATEGIES; REPRODUCTIVE EFFORT; TRADE-OFFS; EVOLUTIONARY ECOLOGY; INFECTIOUS-DISEASES; ANTIBODY RESPONSIVENESS; TEMPORAL VARIATION; ALASKAN MOOSE; TREE SWALLOWS; MULE DEER AB Natural selection favors individuals that respond with effective and appropriate immune responses to macro or microparasites. Animals living in populations close to ecological carrying capacity experience increased intraspecific competition, and as a result are often in poor nutritional condition. Nutritional condition, in turn, affects the amount of endogenous resources that are available for investment in immune function. Our objective was to understand the relationship between immune function and density dependence mediated by trade-offs between immune function, nutritional condition, and reproduction. To determine how immune function relates to density-dependent processes, we quantified bacteria killing ability, hemolytic-complement activity, and nutritional condition of North American elk (Cervus elaphus) from populations maintained at experimentally high-and low-population densities. When compared with elk from the low-density population, those from the high-density population had higher bacteria killing ability and hemolytic-complement activity despite their lower nutritional condition. Similarly, when compared with adults, yearlings had higher bacteria killing ability, higher hemolytic-complement activity, and lower nutritional condition. Pregnancy status and lactational status did not change either measure of constitutive immunity. Density-dependent processes affected both nutritional condition and investment in constitutive immune function. Although the mechanism for how density affects immunity is ambiguous, we hypothesize two possibilities: (i) individuals in higher population densities and in poorer nutritional condition invested more into constitutive immune defenses, or (ii) had higher parasite loads causing higher induced immune responses. Those explanations are not mutually exclusive, and might be synergistic, but overall our results provide stronger support for the hypothesis that animals in poorer nutritional condition invest more in constitutive immune defenses then animals in better nutritional condition. This intriguing hypothesis should be investigated further within the larger framework of the cost and benefit structure of immune responses. C1 [Downs, Cynthia J.; Stewart, Kelley M.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Dick, Brian L.] US Forest Serv, Pacific NW Res Stn, La Grande, OR USA. RP Downs, CJ (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. EM cdowns@unr.edu FU Nevada Agriculture Experimental Station; University of Nevada Reno; US Forest Service FX This study was supported by a Hatch grant awarded by Nevada Agriculture Experimental Station and funding from the University of Nevada Reno, and US Forest Service grant awarded to KMS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 96 TC 2 Z9 2 U1 0 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2015 VL 10 IS 5 AR UNSP e0125586 DI 10.1371/journal.pone.0125586 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI7CV UT WOS:000354921400036 PM 25992627 ER PT J AU Ronquist, F Nieves-Aldrey, JL Buffington, ML Liu, ZW Liljeblad, J Nylander, JAA AF Ronquist, Fredrik Nieves-Aldrey, Jose-Luis Buffington, Matthew L. Liu, Zhiwei Liljeblad, Johan Nylander, Johan A. A. TI Phylogeny, Evolution and Classification of Gall Wasps: The Plot Thickens SO PLOS ONE LA English DT Article ID AUSTRALIAN THRASORINAE HYMENOPTERA; FIGITIDAE HYMENOPTERA; GALLWASPS HYMENOPTERA; OAK GALLWASPS; CYNIPIDAE; CYNIPOIDEA; GENUS; REVISION; SUBFAMILY; INQUILINE AB Gall wasps (Cynipidae) represent the most spectacular radiation of gall-inducing insects. In addition to true gall formers, gall wasps also include phytophagous inquilines, which live inside the galls induced by gall wasps or other insects. Here we present the first comprehensive molecular and total-evidence analyses of higher-level gall wasp relationships. We studied more than 100 taxa representing a rich selection of outgroups and the majority of described cynipid genera outside the diverse oak gall wasps (Cynipini), which were more sparsely sampled. About 5 kb of nucleotide data from one mitochondrial (COI) and four nuclear (28S, LWRh, EF1alpha F1, and EF1alpha F2) markers were analyzed separately and in combination with morphological and life-history data. According to previous morphology-based studies, gall wasps evolved in the Northern Hemisphere and were initially herb gallers. Inquilines originated once from gall inducers that lost the ability to initiate galls. Our results, albeit not conclusive, suggest a different scenario. The first gall wasps were more likely associated with woody host plants, and there must have been multiple origins of gall inducers, inquilines or both. One possibility is that gall inducers arose independently from inquilines in several lineages. Except for these surprising results, our analyses are largely consistent with previous studies. They confirm that gall wasps are conservative in their host-plant preferences, and that herb-galling lineages have radiated repeatedly onto the same set of unrelated host plants. We propose a revised classification of the family into twelve tribes, which are strongly supported as monophyletic across independent datasets. Four are new: Aulacideini, Phanacidini, Diastrophini and Ceroptresini. We present a key to the tribes and discuss their morphological and biological diversity. Until the relationships among the tribes are resolved, the origin and early evolution of gall wasps will remain elusive. C1 [Ronquist, Fredrik; Nylander, Johan A. A.] Swedish Museum Nat Hist, Dept Bioinformat & Genet, S-10405 Stockholm, Sweden. [Nieves-Aldrey, Jose-Luis] CSIC, Museo Nacl Ciencias Nat, Dept Biodiversidad & Biol Evolut, E-28006 Madrid, Spain. [Buffington, Matthew L.] USDA, Smithsonian Inst, Systemat Entomol Lab, Washington, DC 20250 USA. [Liu, Zhiwei] Eastern Illinois Univ, Dept Biol Sci, Charleston, IL 61920 USA. [Liljeblad, Johan] Swedish Univ Agr Sci, Swedish Species Informat Ctr, Uppsala, Sweden. [Nylander, Johan A. A.] Linkoping Univ, Bioinformat Infrastruct Life Sci, Linkoping, Sweden. RP Ronquist, F (reprint author), Swedish Museum Nat Hist, Dept Bioinformat & Genet, S-10405 Stockholm, Sweden. EM fredrik.ronquist@nrm.se RI Ronquist, Fredrik/Q-2013-2015 FU Swedish Research Council [20115622]; Spanish Ministry of Science and Innovation [CGL-2009-10111, CGL-2010-15786]; Kalbfleisch Fellowship, American Museum of Natural History; Boyd Scholarship, The Field Museum; National Science Foundation [1K12 Gm00708]; (HymAToL), National Science Foundation [DEB-0337220] FX This work received support from: 20115622, Swedish Research Council (http://vr.se): FR. CGL-2009-10111 and CGL-2010-15786, Spanish Ministry of Science and Innovation (http://www.idi.mineco.gob.es): JLNA. Kalbfleisch Fellowship, American Museum of Natural History (http://www.amnh.org): ZL. Boyd Scholarship, The Field Museum (http://www.fieldmuseum.org): ZL. Training Grant #1K12 Gm00708, National Science Foundation (http://www.nsf.gov): ZL. DEB-0337220 (HymAToL), National Science Foundation (http://www.nsf.gov): FR MB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 8 Z9 11 U1 3 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2015 VL 10 IS 5 AR e0123301 DI 10.1371/journal.pone.0123301 PG 40 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI7CV UT WOS:000354921400009 PM 25993346 ER PT J AU Thavarajah, D Thavarajah, P Vial, E Gebhardt, M Lacher, C Kumar, S Combs, GF AF Thavarajah, Dil Thavarajah, Pushparajah Vial, Eric Gebhardt, Mary Lacher, Craig Kumar, Shiv Combs, Gerald F. TI Will selenium increase lentil (Lens culinaris Medik) yield and seed quality? SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Lentil (Lens culinaris); selenium; yield increase; fertilization; biofortification; selenoproteins ID ANTIOXIDANT ACTIVITY; HIGHER-PLANTS; HUMAN HEALTH; L.; BIOFORTIFICATION; IRRADIATION; ENVIRONMENT; TOXICITY; GENOTYPE; GROWTH AB Lentil (Lens culinaris Medik), a nutritious traditional pulse crop, has been experiencing a declining area of production in South East Asia, due to lower yields, and marginal soils. The objective of this study was to determine whether selenium (Se) fertilization can increase lentil yield, productivity, and seed quality (both seed Se concentration and speciation). Selenium was provided to five lentil accessions as selenate or selenite by foliar or soil application at rates of 0, 10, 20, or 30 kg Se/ha and the resulting lentil biomass, grain yield, seed Se concentration, and Se speciation was determined. Seed Se concentration was measured using inductively coupled plasma optical emission spectrometry (ICP-OES) after acid digestion. Seed Se speciation was measured using ICP-mass spectrometry with a high performance liquid chromatography (ICP-MS-LC) system. Foliar application of Se significantly increased lentil biomass (5586 vs. 7361 kg/ha), grain yield (1732 vs. 2468 kg/ha), and seed Se concentrations (0.8 vs. 2.4 mu g/g) compared to soil application. In general, both application methods and both forms of Se increased concentrations of organic Se forms (selenocysteine and selenomethionine) in lentil seeds. Not surprisingly, the high yielding CDC Redberry had the highest levels of biomass and grain yield of all varieties evaluated. Eston, ILL505, and CDC Robin had the greatest responses to Se fertilization with respect to both grain yield, seed Se concentration and speciation; thus, use of these varieties in areas with low-Se soils might require Se fertilization to reach yield potentials. C1 [Thavarajah, Dil; Thavarajah, Pushparajah] Clemson Univ, Dept Agr & Environm Sci, Clemson, SC 29634 USA. [Vial, Eric; Gebhardt, Mary] N Dakota State Univ, Fargo, ND 58105 USA. [Lacher, Craig; Combs, Gerald F.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Kumar, Shiv] Int Ctr Agr Res Dry Areas, Biodivers & Integrated Gene Management Program, Rabat, Morocco. RP Thavarajah, D (reprint author), Clemson Univ, Dept Agr & Environm Sci, 130 McGinty Court,270 Poole Agr Ctr, Clemson, SC 29634 USA. EM dthavar@clemson.edu FU Northern Pulse Growers Association, Bismarck, ND, USA; Biodiversity and Integrated Gene Management Program, International Centre for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco; Clemson University, Clemson, SC, USA FX Support for this research was provided by the Northern Pulse Growers Association, Bismarck, ND, USA, Biodiversity and Integrated Gene Management Program, International Centre for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco, and Clemson University, Clemson, SC, USA. NR 34 TC 5 Z9 5 U1 1 U2 14 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 19 PY 2015 VL 6 AR 356 DI 10.3389/fpls.2015.00356 PG 9 WC Plant Sciences SC Plant Sciences GA CL4XU UT WOS:000356961500001 PM 26042141 ER PT J AU Zhang, Y Liu, ZL Song, MZ AF Zhang, Yang Liu, Z. Lewis Song, Mingzhou TI ChiNet uncovers rewired transcription subnetworks in tolerant yeast for advanced biofuels conversion SO NUCLEIC ACIDS RESEARCH LA English DT Article ID GENE SET ENRICHMENT; LIGNOCELLULOSIC INHIBITORY COMPOUND; COMPARATIVE PROTEOMIC ANALYSIS; SACCHAROMYCES-CEREVISIAE; ETHANOLOGENIC YEAST; BIOLOGICAL THEMES; SUB-NETWORK; EXPRESSION; PROTEIN; STRESS AB Analysis of rewired upstream subnetworks impacting downstream differential gene expression aids the delineation of evolving molecular mechanisms. Cumulative statistics based on conventional differential correlation are limited for subnetwork rewiring analysis since rewiring is not necessarily equivalent to change in correlation coefficients. Here we present a computational method ChiNet to quantify subnetwork rewiring by statistical heterogeneity that enables detection of potential genotype changes causing altered transcription regulation in evolving organisms. Given a differentially expressed downstream gene set, ChiNet backtracks a rewired upstream subnetwork from a super-network including gene interactions known to occur under various molecular contexts. We benchmarked ChiNet for its high accuracy in distinguishing rewired artificial subnetworks, in silico yeast transcription-metabolic subnetworks, and rewired transcription subnetworks for Candida albicans versus Saccharomyces cere-visiae, against two differential-correlation based subnetwork rewiring approaches. Then, using transcriptome data from tolerant S. cerevisiae strain NRRL Y-50049 and a wild-type intolerant strain, ChiNet identified 44 metabolic pathways affected by rewired transcription subnetworks anchored to major adaptively activated transcription factor genes YAP1, RPN4, SFP1 and ROX1, in response to toxic chemical challenges involved in lignocellulose-to-biofuels conversion. These findings support the use of ChiNet in rewiring analysis of subnetworks where differential interaction patterns resulting from divergent nonlinear dynamics abound. C1 [Zhang, Yang; Song, Mingzhou] New Mexico State Univ, Dept Comp Sci, Las Cruces, NM 88003 USA. [Liu, Z. Lewis] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Song, MZ (reprint author), New Mexico State Univ, Dept Comp Sci, Las Cruces, NM 88003 USA. EM ZLewis.Liu@ars.usda.gov; joemsong@cs.nmsu.edu OI Song, Mingzhou/0000-0002-6883-6547 FU USDA National Research Initiative [2006-35504-17359]; NIH National Cancer Institute [1U54CA132383]; NSF CREST Center for Bioinformatics and Computational Biology [HRD-0420407]; NSF MRI [CNS-1337884]; NIH New Mexico IDeA Networks of Biomedical Research Excellence [2P20GM103451-14]; NIH Mountain West Clinical Translational Research [1U54GM104944-2]; NIH [1U54GM104944-2] FX USDA National Research Initiative [2006-35504-17359, in part], NIH National Cancer Institute [1U54CA132383]; NSF CREST Center for Bioinformatics and Computational Biology [HRD-0420407]; NSF MRI [CNS-1337884]; NIH New Mexico IDeA Networks of Biomedical Research Excellence [2P20GM103451-14]; NIH Mountain West Clinical Translational Research [1U54GM104944-2]. Funding for open access charge: NIH [1U54GM104944-2]. NR 76 TC 2 Z9 2 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD MAY 19 PY 2015 VL 43 IS 9 BP 4393 EP 4407 DI 10.1093/nar/gkv358 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CK0XK UT WOS:000355928800009 PM 25897127 ER PT J AU Pham, AT Harris, DK Buck, J Hoskins, A Serrano, J Abdel-Haleem, H Cregan, P Song, QJ Boerma, HR Li, ZL AF Pham, Anh-Tung Harris, Donna K. Buck, James Hoskins, Aaron Serrano, Jonathan Abdel-Haleem, Hussein Cregan, Perry Song, Qijian Boerma, H. Roger Li, Zenglu TI Fine Mapping and Characterization of Candidate Genes that Control Resistance to Cercospora sojina K. Hara in Two Soybean Germplasm Accessions SO PLOS ONE LA English DT Article ID FROGEYE LEAF-SPOT; INHIBITOR FUNGICIDES; SSR MARKERS; REGION; REGISTRATION; IDENTIFICATION; INHERITANCE; QUINONE; DISEASE; RACE-5 AB Frogeye leaf spot (FLS), caused by the fungus Cercospora sojina K. Hara, may cause a significant yield loss to soybean growers in regions with a warm and humid climate. Two soybean accessions, PI 594891 and PI 594774, were identified to carry a high level of resistance similar to that conditioned by the Rcs3 gene in 'Davis'. Previously, we reported that the resistance to FLS in these two plant introductions (PIs) was controlled by a novel gene (s) on chromosome 13 that is different from Rcs3. To fine-map the novel FLS resistance gene(s) in these two PIs, F-2:3 seeds from the crosses between PI 594891 and PI 594774, and the FLS susceptible genotype 'Blackhawk' were genotyped with SNP markers that were designed based on the SoySNP50k iSelect BeadChip data to identify recombinant events and locate candidate genes. Analysis of lines possessing key recombination events helped narrow down the FLS-resistance genomic region in PI 594891 from 3.3 Mb to a 72.6 kb region with five annotated genes. The resistance gene in PI 594774 was fine-mapped into a 540 kb region that encompasses the 72.6 kb region found in PI 594891. Sequencing five candidate genes in PI 594891 identified three genes that have several mutations in the promoter, intron, 5', and 3' UTR regions. qPCR analysis showed a difference in expression levels of these genes in both lines compared to Blackhawk in the presence of C. sojina. Based on phenotype, genotype and haplotype analysis results, these two soybean accessions might carry different resistance alleles of the same gene or two different gene(s). The identified SNPs were used to develop Kompetitive Allele Specific PCR (KASP) assays to detect the resistance alleles on chromosome 13 from the two PIs for marker-assisted selection. C1 [Pham, Anh-Tung; Harris, Donna K.; Hoskins, Aaron; Serrano, Jonathan; Abdel-Haleem, Hussein; Boerma, H. Roger; Li, Zenglu] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Pham, Anh-Tung; Harris, Donna K.; Hoskins, Aaron; Serrano, Jonathan; Abdel-Haleem, Hussein; Boerma, H. Roger; Li, Zenglu] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Buck, James] Univ Georgia, Dept Plant Pathol, Griffin, GA USA. [Cregan, Perry; Song, Qijian] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville, MD USA. RP Li, ZL (reprint author), Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. EM zli@uga.edu NR 55 TC 2 Z9 2 U1 5 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2015 VL 10 IS 5 AR UNSP e0126753 DI 10.1371/journal.pone.0126753 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI7BT UT WOS:000354918600043 PM 25993056 ER PT J AU Dogramaci, M Foley, ME Horvath, DP Hernandez, AG Khetani, RS Fields, CJ Keating, KM Mikel, MA Anderson, JV AF Dogramaci, Muenevver Foley, Michael E. Horvath, David P. Hernandez, Alvaro G. Khetani, Radhika S. Fields, Christopher J. Keating, Kathleen M. Mikel, Mark A. Anderson, James V. TI Glyphosate's impact on vegetative growth in leafy spurge identifies molecular processes and hormone cross-talk associated with increased branching SO BMC GENOMICS LA English DT Article DE Bud dormancy; Branching; Glyphosate; Phytohormones; Shikimate; Transcriptomics; RNAseq ID UNDERGROUND ADVENTITIOUS BUDS; GRASS ALOPECURUS-MYOSUROIDES; HORSEWEED CONYZA-CANADENSIS; MILKWEED ASCLEPIAS-SYRIACA; EUPHORBIA-ESULA; SHIKIMATE ACCUMULATION; HERBICIDE RESISTANCE; ARABIDOPSIS-THALIANA; AUXIN BIOSYNTHESIS; MASS-SPECTROMETRY AB Background: Leafy spurge (Euphorbia esula) is a perennial weed that is considered glyphosate tolerant, which is partially attributed to escape through establishment of new vegetative shoots from an abundance of underground adventitious buds. Leafy spurge plants treated with sub-lethal concentrations of foliar-applied glyphosate produce new vegetative shoots with reduced main stem elongation and increased branching. Processes associated with the glyphosate-induced phenotype were determined by RNAseq using aerial shoots derived from crown buds of glyphosate-treated and -untreated plants. Comparison between transcript abundance and accumulation of shikimate or phytohormones (abscisic acid, auxin, cytokinins, and gibberellins) from these same samples was also done to reveal correlations. Results: Transcriptome assembly and analyses confirmed differential abundance among 12,918 transcripts (FDR <= 0.05) and highlighted numerous processes associated with shoot apical meristem maintenance and stem growth, which is consistent with the increased number of actively growing meristems in response to glyphosate. Foliar applied glyphosate increased shikimate abundance in crown buds prior to decapitation of aboveground shoots, which induces growth from these buds, indicating that 5-enolpyruvylshikimate 3-phosphate (EPSPS) the target site of glyphosate was inhibited. However, abundance of shikimate was similar in a subsequent generation of aerial shoots derived from crown buds of treated and untreated plants, suggesting EPSPS is no longer inhibited or abundance of shikimate initially observed in crown buds dissipated over time. Overall, auxins, gibberellins (precursors and catabolites of bioactive gibberellins), and cytokinins (precursors and bioactive cytokinins) were more abundant in the aboveground shoots derived from glyphosate treated plants. Conclusion: Based on the overall data, we propose that the glyphosate-induced phenotype resulted from complex interactions involving shoot apical meristem maintenance, hormone biosynthesis and signaling (auxin, cytokinins, gibberellins, and strigolactones), cellular transport, and detoxification mechanisms. C1 [Dogramaci, Muenevver; Foley, Michael E.; Horvath, David P.; Anderson, James V.] ARS, USDA, Sunflower & Plant Biol Res, Fargo, ND 58102 USA. [Hernandez, Alvaro G.; Khetani, Radhika S.; Fields, Christopher J.; Keating, Kathleen M.] Univ Illinois, WM Keck Ctr Comparat & Funct Genom, Urbana, IL 61801 USA. [Mikel, Mark A.] Univ Illinois, Inst Genom Biol 2608, Dept Crop Sci, Urbana, IL 61801 USA. [Mikel, Mark A.] Univ Illinois, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USA. RP Anderson, JV (reprint author), ARS, USDA, Sunflower & Plant Biol Res, Fargo, ND 58102 USA. EM james.anderson@ars.usda.gov OI Keating, Kathleen/0000-0001-8289-0083; Fields, Christopher/0000-0002-7749-5844 NR 97 TC 5 Z9 5 U1 5 U2 39 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 19 PY 2015 VL 16 AR 395 DI 10.1186/s12864-015-1627-9 PG 22 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CI6CH UT WOS:000354845800002 PM 25986459 ER PT J AU Castro, EB Ochoa, R Feres, RJF Beard, JJ Bauchan, GR AF Castro, Elizeu B. Ochoa, Ronald Feres, Reinaldo J. F. Beard, Jennifer J. Bauchan, Gary R. TI Reinstatement of the genus Colopalpus Pritchard and Baker (1958) and re-description of Colopalpus matthyssei Pritchard and Baker (1958), the type species of the genus (Acari, Tenuipalpidae) SO INTERNATIONAL JOURNAL OF ACAROLOGY LA English DT Article DE flat mites; taxonomy; Tenuipalpus; systematics; Brevipalpus; Philippines ID SPIDER-MITES AB We discuss the taxonomic history of the genus Colopalpus Pritchard and Baker 1958, and redescribe the female, male and deutonymph of the type species, C. matthyssei (Pritchard and Baker). We provide previously unknown morphological data for C. matthyssei, in addition to complementary information on the morphology of C. nambii (Castro and Feres) obtained using low temperature scanning electron microscopy (LT-SEM). Our results show that both C. matthyssei and C. nambii share characters with the genera Tenuipalpus and Brevipalpus. Based on these findings, we propose that Colopalpus be reinstated as a valid genus. We discuss the characters shared by Colopalpus, Tenuipalpus and Brevipalpus, and also provide a list of species that are transferred to the genus Colopalpus. [GRAPHICS] C1 [Castro, Elizeu B.] UNESP Univ Estadual Paulista, Programa Pos Grad Biol Anim, Araraquara, SP, Brazil. [Ochoa, Ronald] ARS, SEL, USDA, BARC, Beltsville, MD USA. [Feres, Reinaldo J. F.] UNESP, Dept Zool & Bot, Sao Jose Do Rio Preto, Brazil. [Beard, Jennifer J.] Queensland Museum, South Brisbane, Australia. [Bauchan, Gary R.] ARS, ECMU, USDA, BARC, Beltsville, MD USA. RP Castro, EB (reprint author), UNESP Univ Estadual Paulista, Programa Pos Grad Biol Anim, Campus Sao Jose do Rio Preto, Araraquara, SP, Brazil. EM elizeu_unesp@yahoo.com.br; ron.ochoa@ars.usda.gov; reinaldo@ibilce.unesp.br; jenny.beard@qm.qld.gov.au; gary.bauchan@ars.usda.gov RI Castro, Elizeu/A-9915-2015 OI Castro, Elizeu/0000-0002-8056-6893 FU "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) [BEX 7768/13-3]; "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) [303435/2013-5]; Australian Biological Resources Study (ABRS), Australian Government [RF214-23] FX This study was supported by the "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) [Proc. no BEX 7768/13-3] and "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) [Proc. no 303435/2013-5], by fellowship and research grant to first and third authors, respectively. Fourth author was funded by the Australian Biological Resources Study (ABRS) [grant number RF214-23], Australian Government. NR 20 TC 3 Z9 3 U1 0 U2 3 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0164-7954 EI 1945-3892 J9 INT J ACAROL JI Int. J. Acarol. PD MAY 19 PY 2015 VL 41 IS 4 BP 310 EP 328 DI 10.1080/01647954.2015.1031276 PG 19 WC Entomology SC Entomology GA CH5AH UT WOS:000354045600009 ER PT J AU Temesgen, H Affleck, D Poudel, K Gray, A Sessions, J AF Temesgen, Hailemariam Affleck, David Poudel, Krishna Gray, Andrew Sessions, John TI A review of the challenges and opportunities in estimating above ground forest biomass using tree-level models SO SCANDINAVIAN JOURNAL OF FOREST RESEARCH LA English DT Review DE biomass allometries; uncertainity; sampling error ID ALLOMETRIC RELATIONS; PONDEROSA PINE; DOUGLAS-FIR; EQUATIONS; FERTILIZATION; INVENTORY; STANDS; CARBON; VOLUME AB Accurate biomass measurements and analyses are critical components in quantifying carbon stocks and sequestration rates, assessing potential impacts due to climate change, locating bio-energy processing plants, and mapping and planning fuel treatments. To this end, biomass equations will remain a key component of future carbon measurements and estimation. As researchers in biomass and carbon estimation, we review the present scenario of aboveground biomass estimation, focusing particularly on estimation using tree-level models and identify some cautionary points that we believe will improve the accuracy of biomass and carbon estimates to meet societal needs. In addition, we discuss the critical challenges in developing or calibrating tree biomass models and opportunities for improved biomass. Some of the opportunities to improve biomass estimate include integration of taper and other attributes and combining different data sources. Biomass estimation is a complex process, when possible, we should make use of already available resources such as wood density and forest inventory databases. Combining different data-sets for model development and using independent data-sets for model verification will offer opportunities to improve biomass estimation. Focus should also be made on belowground biomass estimation to accurately estimate the full forest contribution to carbon sequestration. In addition, we suggest developing comprehensive biomass estimation methods that account for differences in site and stand density and improve forest biomass modeling and validation at a range of spatial scales. C1 [Temesgen, Hailemariam; Poudel, Krishna; Sessions, John] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97333 USA. [Affleck, David] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Gray, Andrew] US Forest Serv, Vegetat Monitoring Sci & Applicat Team, Resource Monitoring & Assessment Program, USDA,PNW Res Stn, Corvallis, OR 97331 USA. RP Temesgen, H (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97333 USA. EM Hailemariam.Temesgen@oregonstate.edu FU Forest Inventory and Analysis program, Pacific Northwest Research Station, United States Forest Service (USFS); USFS national office FX We gratefully acknowledge the support provided by the Forest Inventory and Analysis program, Pacific Northwest Research Station, United States Forest Service (USFS). The authors also acknowledge the support of the USFS national office and its regional program directors in instituting a review of FIA biomass sampling and modeling protocols with the collaboration of academic and private-sector cooperators. NR 49 TC 19 Z9 19 U1 10 U2 59 PU TAYLOR & FRANCIS AS PI OSLO PA KARL JOHANS GATE 5, NO-0154 OSLO, NORWAY SN 0282-7581 EI 1651-1891 J9 SCAND J FOREST RES JI Scand. J. Forest Res. PD MAY 19 PY 2015 VL 30 IS 4 BP 326 EP 335 DI 10.1080/02827581.2015.1012114 PG 10 WC Forestry SC Forestry GA CF9OE UT WOS:000352893900008 ER PT J AU D'Aquila, T Sirohi, D Grabowski, JM Hedrick, VE Paul, LN Greenberg, AS Kuhn, RJ Buhman, KK AF D'Aquila, Theresa Sirohi, Devika Grabowski, Jeffrey M. Hedrick, Victoria E. Paul, Lake N. Greenberg, Andrew S. Kuhn, Richard J. Buhman, Kimberly K. TI Characterization of the Proteome of Cytoplasmic Lipid Droplets in Mouse Enterocytes after a Dietary Fat Challenge SO PLOS ONE LA English DT Article ID TRIGLYCERIDE-RICH LIPOPROTEINS; ACYL-COA SYNTHETASES; LARGE GENE LISTS; A-IV EXPRESSION; DIACYLGLYCEROL ACYLTRANSFERASE-2; TRIACYLGLYCEROL SYNTHESIS; ENDOPLASMIC-RETICULUM; PROTEINS; SECRETION; CELLS AB Dietary fat absorption by the small intestine is a multistep process that regulates the uptake and delivery of essential nutrients and energy. One step of this process is the temporary storage of dietary fat in cytoplasmic lipid droplets (CLDs). The storage and mobilization of dietary fat is thought to be regulated by proteins that associate with the CLD; however, mechanistic details of this process are currently unknown. In this study we analyzed the proteome of CLDs isolated from enterocytes harvested from the small intestine of mice following a dietary fat challenge. In this analysis we identified 181 proteins associated with the CLD fraction, of which 37 are associated with known lipid related metabolic pathways. We confirmed the localization of several of these proteins on or around the CLD through confocal and electron microscopy, including perilipin 3, apolipoprotein A-IV, and acyl-CoA synthetase long-chain family member 5. The identification of the enterocyte CLD proteome provides new insight into potential regulators of CLD metabolism and the process of dietary fat absorption. C1 [D'Aquila, Theresa; Buhman, Kimberly K.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Sirohi, Devika; Grabowski, Jeffrey M.; Kuhn, Richard J.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Sirohi, Devika; Hedrick, Victoria E.; Paul, Lake N.; Kuhn, Richard J.] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA. [Grabowski, Jeffrey M.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Greenberg, Andrew S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Buhman, KK (reprint author), Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. EM kbuhman@purdue.edu FU Indiana Clinical and Translational Sciences Institute; National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award [UL1TR001108]; American Diabetes Association, Innovation Award [7-13-IN-05]; National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R01 DK098606]; National Institutes of Health, NIDDK [P30 DK046200]; National Institutes of Health, National Institute of Environmental Health Science [U01 ES020958]; Purdue Research Foundation, Graduate Fellowship Award; U.S. Department of Agriculture [58-1950-4-003] FX This project was supported by the Indiana Clinical and Translational Sciences Institute, by Grant # UL1TR001108 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award (KKB), Grant # 7-13-IN-05 by the American Diabetes Association, Innovation Award (KKB), Grant # R01 DK098606 from the National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) (KKB, ASG), Grant # P30 DK046200 National Institutes of Health, NIDDK (ASG), Grant # U01 ES020958 National Institutes of Health, National Institute of Environmental Health Science (ASG), and the Purdue Research Foundation, Graduate Fellowship Award (KKB, TD). This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-4-003 (ASG). Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 66 TC 7 Z9 7 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 18 PY 2015 VL 10 IS 5 AR e0126823 DI 10.1371/journal.pone.0126823 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI7BG UT WOS:000354917300055 PM 25992653 ER PT J AU Zheng, Y Wang, Y Wu, J Ding, B Fei, ZJ AF Zheng, Yi Wang, Ying Wu, Jian Ding, Biao Fei, Zhangjun TI A dynamic evolutionary and functional landscape of plant phased small interfering RNAs SO BMC BIOLOGY LA English DT Article DE Evolution; Function; Plant phasiRNAs; Protein-coding genes ID TRANS-ACTING SIRNAS; ALGA CHLAMYDOMONAS-REINHARDTII; PHYSCOMITRELLA-PATENS; ARABIDOPSIS-THALIANA; MESSENGER-RNAS; DICER-LIKE; MICRORNA; BIOGENESIS; EXPRESSION; SECONDARY AB Background: Secondary, phased small interfering RNAs (phasiRNAs) derived from protein-coding or noncoding loci (PHAS) are emerging as a new type of regulators of gene expression in plants. However, the evolution and function of these novel siRNAs in plant species remain largely unexplored. Results: We systematically analyzed PHAS loci in 23 plant species covering major phylogenetic groups spanning alga, moss, gymnosperm, basal angiosperm, monocot, and dicot. We identified over 3,300 PHAS loci, among which similar to 1,600 were protein-coding genes. Most of these PHAS loci were novel and clade- or species-specific and showed distinct expression patterns in association with particular development stages, viral infection, or abiotic stresses. Unexpectedly, numerous PHAS loci produced phasiRNAs from introns or exon-intron junction regions. Our comprehensive analysis suggests that phasiRNAs predominantly regulate protein-coding genes from which they are derived and genes from the same families of the phasiRNA-deriving genes, in contrast to the dominant trans-regulatory mode of miRNAs. The stochastic occurrence of many PHAS loci in the plant kingdom suggests their young evolutionary origins. Conclusions: Our study discovered an unprecedented diversity of protein-coding genes that produce phasiRNAs in a wide variety of plants, and set a kingdom-wide foundation for investigating the novel roles of phasiRNAs in shaping phenotype diversities of plants. C1 [Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Wang, Ying; Ding, Biao] Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA. [Wang, Ying; Wu, Jian; Ding, Biao] Ohio State Univ, Ctr RNA Biol, Columbus, OH 43210 USA. [Wu, Jian; Ding, Biao] Ohio State Univ, Mol Cellular & Dev Biol Program, Columbus, OH 43210 USA. [Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Ding, B (reprint author), Ohio State Univ, Dept Mol Genet, Columbus, OH 43210 USA. EM ding.35@osu.edu; zf25@cornell.edu RI Wang, Ying/Q-2205-2015; Zheng, Yi/F-6150-2016; OI Wang, Ying/0000-0002-9659-977X; Zheng, Yi/0000-0002-8042-7770; Fei, Zhangjun/0000-0001-9684-1450 FU Agriculture and Food Research Initiative Competitive Grant of the USDA National Institute of Food and Agriculture [2014-67013-21550]; National Science Foundation [IOS-1051655, IOS-0923312, IOS-1313887]; Ohio Plant Biotechnology Consortium FX We thank Dr Blake Meyers for helpful discussions. We are grateful to Dr Yi Li at Peking University for making the small RNA library data from virus-infected and healthy rice plants available to us. This work was supported by grants from the Agriculture and Food Research Initiative Competitive Grant of the USDA National Institute of Food and Agriculture to BD and ZF (2014-67013-21550), National Science Foundation to BD (IOS-1051655) and to ZF (IOS-0923312 and IOS-1313887), and Ohio Plant Biotechnology Consortium to BD. NR 60 TC 9 Z9 9 U1 0 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1741-7007 J9 BMC BIOL JI BMC Biol. PD MAY 16 PY 2015 VL 13 AR UNSP 32 DI 10.1186/s12915-015-0142-4 PG 15 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CK0NM UT WOS:000355901900001 PM 25980406 ER PT J AU Hayes, TR Lyon, PA Barnes, C Trabue, S Benny, PD AF Hayes, Thomas R. Lyon, Patrice A. Barnes, Charles Trabue, Steven Benny, Paul D. TI Development of ring substituted pyridine based chelates for fac-[M-I(CO)(3)](+) (M=Re, Tc-99m) based radiopharmaceuticals SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Meeting Abstract C1 [Hayes, Thomas R.; Lyon, Patrice A.; Benny, Paul D.] Washignton State Univ, Chem, Pullman, WA USA. [Barnes, Charles] Univ Missouri, Dept Chem, Columbia, MO 65211 USA. [Trabue, Steven] USDA, Natl Soil Tilth Lab, Ames, IA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0362-4803 EI 1099-1344 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD MAY 15 PY 2015 VL 58 SU 1 MA 142 BP S142 EP S142 PG 1 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA DD5GH UT WOS:000369950200143 ER PT J AU Cleveland, BM Weber, GM AF Cleveland, Beth M. Weber, Gregory M. TI Effects of sex steroids on expression of genes regulating growth-related mechanisms in rainbow trout (Oncorhynchus mykiss) SO GENERAL AND COMPARATIVE ENDOCRINOLOGY LA English DT Article DE Fish; Steroid; Multiplex; Insulin-like growth factor; Reproduction ID IGF-BINDING-PROTEINS; SALMON SALMO-SALAR; MUSCLE-CELL-DIFFERENTIATION; ESTROGEN-RECEPTOR SUBTYPES; PARR-SMOLT TRANSFORMATION; MESSENGER-RNA EXPRESSION; FACTOR-I; SKELETAL-MUSCLE; ATLANTIC SALMON; FACTOR SYSTEM AB Effects of a single injection of 17 beta-estradiol (E2), testosterone (T), or 5 beta-dihydrotestosterone (DHT) on expression of genes central to the growth hormone (GH)/insulin-like growth factor (IGF) axis, muscle-regulatory factors, transforming growth factor-beta (TGF beta) superfamily signaling cascade, and estrogen receptors were determined in rainbow trout (Oncorhynchus mykiss) liver and white muscle tissue. In liver in addition to regulating GH sensitivity and IGF production, sex steroids also affected expression of IGF binding proteins, as E2, T, and DHT increased expression of igfbp2b and E2 also increased expression of igfbp2 and igfbp4. Regulation of this system also occurred in white muscle in which E2 increased expression of igf1, igf2, and igfbp5b1, suggesting anabolic capacity may be maintained in white muscle in the presence of E2. In contrast, DHT decreased expression of igfbp5b1. DHT and T decreased expression of myogenin, while other muscle regulatory factors were either not affected or responded similarly for all steroid treatments. Genes within the TGF beta superfamily signaling cascade responded to steroid treatment in both liver and muscle, suggesting a regulatory role for sex steroids in the ability to transmit signals initiated by TGF beta superfamily ligands, with a greater number of genes responding in liver than in muscle. Estrogen receptors were also regulated by sex steroids, with era1 expression increasing for all treatments in muscle, but only E2- and T-treatment in liver. E2 reduced expression of erb2 in liver. Collectively, these data identify how physiological mechanisms are regulated by sex steroids in a manner that promotes the disparate effects of androgens and estrogens on growth in salmonids. Published by Elsevier Inc. C1 [Cleveland, Beth M.; Weber, Gregory M.] USDA ARS, NCCCWA, Kearneysville, WV 25427 USA. RP Cleveland, BM (reprint author), USDA ARS, NCCCWA, 11861 Leetown Rd, Kearneysville, WV 25427 USA. EM beth.cleveland@ars.usda.gov FU Agricultural Research Service Project [1930-31000-010-000D] FX The authors are grateful to Lisa Radler, Jill Birkett, and Aylen Molina for their technical expertise during sample collection and analysis. In addition, we acknowledge technical and animal caretaking contributions from Josh Kretzer, Jenea McGowan, and Kyle Jenkins. Mention of trade names is solely for the purpose of providing accurate information and should not imply product endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. Funding for this study came from the Agricultural Research Service Project 1930-31000-010-000D. NR 101 TC 5 Z9 5 U1 6 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0016-6480 EI 1095-6840 J9 GEN COMP ENDOCR JI Gen. Comp. Endocrinol. PD MAY 15 PY 2015 VL 216 BP 103 EP 115 DI 10.1016/j.ygcen.2014.11.018 PG 13 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CK3KO UT WOS:000356115200013 PM 25482545 ER PT J AU Neill, JD Ridpath, JF Valayudhan, BT AF Neill, John D. Ridpath, Julia F. Valayudhan, Binu T. TI Identification and genome characterization of genotype B and genotype C bovine parainfluenza type 3 viruses isolated in the United States SO BMC VETERINARY RESEARCH LA English DT Article DE Deep sequencing; Virus genome; Paramyxovirus; Virus genotype; Subgenotype ID VIRAL-DIARRHEA-VIRUS; GENE; RNA; EXPRESSION; PROTEIN; RULE AB Background: Bovine parainfluenza 3 viruses (BPI3V) are respiratory pathogens of cattle that cause disease singly but are often associated with bovine respiratory disease complex (BRDC) in conjunction with other viral and bacterial agents. Bovine vaccines currently contain BPI3V to provide protection against the virus, but there is no current information regarding the BPI3V strains that are circulating in the U.S. Results: A project was initiated to sequence archival BPI3V isolates to study viral evolution over time. This was done with a deep sequencing protocol that generated sequences of multiple RNA virus genomes simultaneously. Analysis of the BPI3V sequences revealed that, in addition to the genotype A (BPI3Va) viruses previously described in the United States, there were two additional genotypes of BPI3V circulating that had been described only in Australia (BPI3Vb) and Asia (BPI3Vc). The U.S. BPI3Vb and BPI3Vc isolates showed some divergence from the Australian and Asian strains; the BPI3Vb were 93 % similar to the Australian Q5592 strain and the BPI3Vc viruses were 98 % similar to the 12Q061 strain that was described in South Korea. Overall, the three genotypes were 82 to 84 % identical to each other and 80 % identical to the most similar human PI3V. Cross-neutralization studies using an APHIS/NVSL BPI3V reference serum showed that neutralization titers against the genotype B and C viruses were 4- to >= 16-fold less then the titer against the APHIS BPI3Va reference strain, SF-4. Conclusions: This study clearly demonstrated that BPI3Vb and BPI3Vc strains, previously thought to be foreign to the U.S., are indeed circulating in domestic livestock herds. Based on virus neutralization using polyclonal antisera, there were antigenic differences between viruses from these genotypes and the BPI3Va viruses that are included in currently marketed bovine vaccines. Further study of these viruses is warranted to determine pathogenic potential and cross-protection afforded by vaccination. C1 [Neill, John D.; Ridpath, Julia F.] USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [Valayudhan, Binu T.] Texas A&M Vet Med Diagnost Lab, Amarillo, TX USA. RP Neill, JD (reprint author), USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM john.neill@ars.usda.gov FU USDA; ARS FX This study was financed by USDA, ARS in-house funds. NR 26 TC 4 Z9 5 U1 2 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD MAY 15 PY 2015 VL 11 AR 112 DI 10.1186/s12917-015-0431-8 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CI7AN UT WOS:000354915400001 PM 25976921 ER PT J AU Crim, SM Griffin, PM Tauxe, R Marder, EP Gilliss, D Cronquist, AB Cartter, M Tobin-D'Angelo, M Blythe, D Smith, K Lathrop, S Zansky, S Cieslak, PR Dunn, J Holt, KG Wolpert, B Henao, OL AF Crim, Stacy M. Griffin, Patricia M. Tauxe, Robert Marder, Ellyn P. Gilliss, Debra Cronquist, Alicia B. Cartter, Matthew Tobin-D'Angelo, Melissa Blythe, David Smith, Kirk Lathrop, Sarah Zansky, Shelley Cieslak, Paul R. Dunn, John Holt, Kristin G. Wolpert, Beverly Henao, Olga L. TI Preliminary Incidence and Trends of Infection with Pathogens Transmitted Commonly Through Food - Foodborne Diseases Active Surveillance Network, 10 US Sites, 2006-2014 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID UNITED-STATES C1 [Crim, Stacy M.; Griffin, Patricia M.; Tauxe, Robert; Marder, Ellyn P.; Henao, Olga L.] CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Marder, Ellyn P.] Atlanta Res & Educ Fdn, Atlanta, GA USA. [Gilliss, Debra] Calif Dept Publ Hlth, Richmond, CA USA. [Cronquist, Alicia B.] Colorado Dept Publ Hlth & Environm, Denver, CO USA. [Cartter, Matthew] Connecticut Dept Publ Hlth, Hartford, CT USA. [Tobin-D'Angelo, Melissa] Georgia Dept Publ Hlth, Marietta, GA USA. [Blythe, David] Maryland Dept Hlth & Mental Hyg, Baltimore, MD 21201 USA. [Smith, Kirk] Minnesota Dept Hlth, Minneapolis, MN 55414 USA. [Lathrop, Sarah] Univ New Mexico, Albuquerque, NM 87131 USA. [Zansky, Shelley] New York State Dept Hlth, Albany, NY 12237 USA. [Cieslak, Paul R.] Oregon Hlth Author, Portland, OR USA. [Dunn, John] Tennessee Dept Hlth, Nashville, TN USA. [Holt, Kristin G.] Food Safety & Inspect Serv, USDA, Washington, DC USA. [Wolpert, Beverly] US FDA, Ctr Food Safety & Appl Nutr, Rockville, MD 20857 USA. RP Henao, OL (reprint author), CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. EM ohenao@cdc.gov NR 9 TC 23 Z9 24 U1 1 U2 13 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD MAY 15 PY 2015 VL 64 IS 18 BP 495 EP 499 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CI4AK UT WOS:000354688400003 PM 25974634 ER PT J AU Pan, D Rostagno, MH Ebner, PD Eicher, SD AF Pan, Deng Rostagno, Marcos H. Ebner, Paul D. Eicher, Susan D. TI Differential innate immune responses of bovine peripheral blood leukocytes to Salmonella enterica serovars Dublin, Typhimurium, and Enteritidis SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Bovine; Flow cytometry; Innate immunity; PCR; Pro-inflammatory cytokines ID EPITHELIAL-CELLS; S-TYPHIMURIUM; TNF-ALPHA; INFECTION; EXPRESSION; TRANSPORT; LIVE; INTERLEUKIN-8; CHOLERAESUIS; PRODUCTS AB The majority of Salmonella serovars cause no clinical disease in cattle, while some are associated with severe disease. The objective of the current study was to determine the innate immune responses of bovine peripheral blood leukocytes exposed to Salmonella enterica serovar Dublin (bovine-specific), Salmonella typhimurium (murine adapted, but zoonotic), and Salmonella enteritidis (poultry host-adapted) in 3-week-old calves. All Salmonella exposures increased cell surface CD14 and CD18 regardless of serovar. The greatest CD14 marker mean fluorescence was in monocytes and the greatest mean fluorescent of the marker mean was in neutrophils. Phagocytosis increased with all serovars, but was not different among them. Neutrophils had the greatest marker mean fluorescence for phagocytosis, with all serovars being equal. Oxidative burst increased in all serovars compared to control cells, but were not different among the serovars. Neutrophils and monocytes were similar in the oxidative burst, with limited oxidative burst detected in the primarily lymphocyte population. mRNA expression of TNF-alpha, IL-8, and IL-12, increased above the control cells whereas none of these serovars affected mRNA expression of TLR4. TNF-alpha was greatest in S. enterica and S. typhimurium, compared to Salmonella dublin. In contrast, IL-8 was expressed more in S. dublin than S. typhiurium, with S. Enteriditus intermediary. These results show while cell surface markers, phagocytosis, and oxidative burst were largely unaffected by serovar, cytokine and chemokine expression differed among the Salmonella serovars. It appears that internal responses of the cells differ, rather than cell recognition, creating pathogenicity differences among of the serovars, even in the neonate with developing immunity. Published by Elsevier B.V. C1 [Pan, Deng; Ebner, Paul D.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Pan, Deng; Rostagno, Marcos H.; Eicher, Susan D.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Eicher, SD (reprint author), 125 S Russell St, W Lafayette, IN 47907 USA. EM Susan.Eicher@ars.usda.gov NR 41 TC 1 Z9 1 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD MAY 15 PY 2015 VL 165 IS 1-2 BP 14 EP 21 DI 10.1016/j.vetimm.2015.03.006 PG 8 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA CI8LY UT WOS:000355025000002 PM 25847354 ER PT J AU Griswold, T Herndon, JD Gonzalez, VH AF Griswold, Terry Herndon, James D. Gonzalez, Victor H. TI First record of the orchid bee genus Eufriesea Cockerell (Hymenoptera: Apidae: Euglossini) in the United States SO ZOOTAXA LA English DT Article DE Neotropics; Chihuahuan Desert; range extension; taxonomy ID FLORIDA; FRIESE; MEXICO AB The orchid bee genus Eufriesea Cockerell is documented for the first time from the southwestern United States, extending its apparent range north well beyond its previous tropical/subtropical boundaries. Eufriesea coerulescens (Lepeletier de Saint Fargeau 1841) is recorded from the Guadalupe Mountains of western Texas and southeastern New Mexico, USA. Whether E. coerulescens is resident in the USA, or merely a vagrant from Mexico, remains unclear. Distinctive morphological traits shared with the holotype are enumerated and illustrated. In addition, observations that question the current species concept and distribution of E. coerulescens are provided. C1 [Griswold, Terry] Utah State Univ, USDA ARS Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Gonzalez, Victor H.] Univ Kansas, Undergrad Biol Program, Lawrence, KS 66045 USA. RP Griswold, T (reprint author), Utah State Univ, USDA ARS Bee Biol & Systemat Lab, Logan, UT 84322 USA. EM terry.griswold@ars.usda.gov; james.herndon85@gmail.com; victorgonzab@gmail.com FU Carlsbad Caverns National Park FX We wish to acknowledge the significant support of Carlsbad Caverns National Park and most particularly wildlife biologist Renee West, who initiated our study of the bees of the park, and whose interest and investment made it happen. We thank Harold Ikerd for assistance in implementing the fieldwork, Agniele Touret-Alby (Museum National d'Historie Naturelle, France) for kindly arranging the loan of the type of Eufriesea coerulescens, and Ricardo Ayala and an anonymous reviewer for helpful comments that significantly improved the manuscript. NR 20 TC 1 Z9 1 U1 0 U2 5 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAY 15 PY 2015 VL 3957 IS 3 BP 342 EP 346 DI 10.11646/zootaxa.3957.3.7 PG 5 WC Zoology SC Zoology GA CI8BZ UT WOS:000354993800007 PM 26249081 ER PT J AU Linde, D Ruiz-Duenas, FJ Fernandez-Fueyo, E Guallar, V Hammel, KE Pogni, R Martinez, AT AF Linde, Dolores Ruiz-Duenas, Francisco J. Fernandez-Fueyo, Elena Guallar, Victor Hammel, Kenneth E. Pogni, Rebecca Martinez, Angel T. TI Basidiomycete DyPs: Genomic diversity, structural-functional aspects, reaction mechanism and environmental significance SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Review DE Dye-decolorizing peroxidases; CDE superfamily; Molecular structure; Reaction mechanism; Catalytic tryptophan; Long-range electron transfer; Substituted anthraquinone breakdown; Ligninolysis ID DYE-DECOLORIZING PEROXIDASE; THANATEPHORUS-CUCUMERIS DEC-1; ELECTRON-TRANSFER PATHWAYS; RHODOCOCCUS-JOSTII RHA1; LIGNIN PEROXIDASE; VERSATILE PEROXIDASE; HETEROLOGOUS EXPRESSION; CRYSTAL-STRUCTURES; MARASMIUS-SCORODONIUS; MANGANESE PEROXIDASE AB The first enzyme with dye-decolorizing peroxidase (DyP) activity was described in 1999 from an arthroconidial culture of the fungus Bjerkandera adusta. However, the first DyP sequence had been deposited three years before, as a peroxidase gene from a culture of an unidentified fungus of the family Polyporaceae (probably Irpex lacteus). Since the first description, fewer than ten basidiomycete DyPs have been purified and characterized, but a large number of sequences are available from genomes. DyPs share a general fold and heme location with chlorite dismutases and other DyP-type related proteins (such as Escherichia coli EfeB), forming the CDE superfamily. Taking into account the lack of an evolutionary relationship with the catalase-peroxidase superfamily, the observed heme pocket similarities must be considered as a convergent type of evolution to provide similar reactivity to the enzyme cofactor. Studies on the Auricularia auricula-judae DyP showed that high-turnover oxidation of anthraquinone type and other DyP substrates occurs via long-range electron transfer from an exposed tryptophan (Trp377, conserved in most basidiomycete DyPs), whose catalytic radical was identified in the H2O2-activated enzyme. The existence of accessory oxidation sites in DyP is suggested by the residual activity observed after site-directed mutagenesis of the above tryptophan. DyP degradation of substituted anthraquinone dyes (such as Reactive Blue 5) most probably proceeds via typical one-electron peroxidase oxidations and product breakdown without a DyP-catalyzed hydrolase reaction. Although various DyPs are able to break down phenolic lignin model dimers, and basidiomycete DyPs also present marginal activity on non-phenolic dimers, a significant contribution to lignin degradation is unlikely because of the low activity on high redox-potential substrates. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license C1 [Linde, Dolores; Ruiz-Duenas, Francisco J.; Fernandez-Fueyo, Elena; Martinez, Angel T.] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain. [Guallar, Victor] Barcelona Supercomp Ctr, Res Program Computat Biol, Joint BSC CRG IRB, E-08034 Barcelona, Spain. [Guallar, Victor] Passeig Lluis Co 23, ICREA, E-08010 Barcelona, Spain. [Hammel, Kenneth E.] US Forest Serv, Madison, WI 53726 USA. [Pogni, Rebecca] Univ Siena, Dept Biotechnol Chem & Pharm, I-53100 Siena, Italy. RP Martinez, AT (reprint author), CSIC, Ctr Invest Biol, Ramiro de Maeztu 9, E-28040 Madrid, Spain. EM ATMartinez@cib.csic.es RI Hammel, Kenneth/G-1890-2011; Ruiz-Duenas, Francisco/L-9837-2015; Guallar, Victor/B-1579-2013; OI Hammel, Kenneth/0000-0002-2935-5847; Ruiz-Duenas, Francisco/0000-0002-9837-5665; Guallar, Victor/0000-0002-4580-1114; Martinez, Angel T/0000-0002-1584-2863 FU INDOX European project [KBBE-2013-7-613549]; Spanish Ministry of Economy and Competitiveness (MINECO) [BIO2011-26694, CTQ2013-48287]; Italian Ministry of Education, University and Research (MIUR); MINECO FX This work was supported by the INDOX (KBBE-2013-7-613549; www.indoxproject.eu) European project, the BIO2011-26694 (HIPOP) and CTQ2013-48287 projects of the Spanish Ministry of Economy and Competitiveness (MINECO), and the PRIN 2009-STNWX3 project of the Italian Ministry of Education, University and Research (MIUR). FJR-D thanks a Ramon y Cajal contract of MINECO. The authors thank Veronica Saez-Jimenez for data on Reactive Blue 5 decolorization by VP and its heme-channel variants. NR 89 TC 12 Z9 13 U1 6 U2 40 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 EI 1096-0384 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD MAY 15 PY 2015 VL 574 SI SI BP 66 EP 74 DI 10.1016/j.abb.2015.01.018 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA CH6MA UT WOS:000354149500008 PM 25637654 ER PT J AU Anderson, CM Ralph, JL Johnson, L Scheett, A Wright, ML Taylor, JY Ohm, JE Uthus, E AF Anderson, Cindy M. Ralph, Jody L. Johnson, LuAnn Scheett, Angela Wright, Michelle L. Taylor, Jacquelyn Y. Ohm, Joyce E. Uthus, Eric TI First trimester vitamin D status and placental epigenomics in preeclampsia among Northern Plains primiparas SO LIFE SCIENCES LA English DT Article DE DNA methylation; Gestational hypertension; Vitamin D ID CULTURED HUMAN SYNCYTIOTROPHOBLASTS; GESTATIONAL HYPERTENSION; NULLIPAROUS WOMEN; D SUPPLEMENTATION; FETAL-DEVELOPMENT; D INSUFFICIENCY; PREGNANT-WOMEN; HEALTH; EPIGENETICS; RISK AB Aims: As maternal vitamin D status has been associated with preeclampsia, the purpose of this study was to determine variations in DNA methylation patterns and associated protein expression in placental genes regulating vitamin D metabolism. Main methods: A convenience sample of 48 pregnant nulliparous women, including 11 later diagnosed with preeclampsia, were recruited in this prospective study. Using a case-control design in two groups of women, we administered a food frequency questionnaire to determine vitamin D dietary intake. Laboratory measures included serum vitamin D levels (25[OH]D), DNA methylation patterns and protein expression in placental genes regulating vitamin D metabolism (la-hydroxylase, CYP27B1; vitamin D receptor, VDR; retinoid X receptor, RXR) from placental tissue collected at delivery among those diagnosed with preeclampsia and those who remained normotensive throughout pregnancy. Key findings: There were no significant differences in vitamin D dietary intake or mean serum 25[OH]D levels, although the proportion of women with deficient 25[OH]D levels was higher in the preeclampsia group (46%) than the normotensive group (20%). Placenta samples from women with preeclampsia also had increased DNA methylation of CYP27B1, VDR and RXR genes with lower protein expression levels limited to RXR. Significance: Hypermethylation of key placental genes involved in vitamin D metabolism suggests uncoupling of processes that may interfere with placentation and availability of vitamin Oat the maternal-fetal interface. (C) 2014 Elsevier Inc. All rights reserved. C1 [Anderson, Cindy M.] Ohio State Univ, Coll Nursing, Columbus, OH 43210 USA. [Ralph, Jody L.] Univ N Dakota, Coll Nursing & Profess Disciplines, Grand Forks, ND 58201 USA. [Johnson, LuAnn; Scheett, Angela; Uthus, Eric] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Wright, Michelle L.] Virginia Commonwealth Univ, Richmond, VA USA. [Taylor, Jacquelyn Y.] Yale Univ, Sch Nursing, Orange, CT USA. [Ohm, Joyce E.] Univ N Dakota, Sch Med & Hlth Sci, Grand Forks, ND 58201 USA. RP Anderson, CM (reprint author), Ohio State Univ, Coll Nursing, 1585 Neil Ave,Newton 346, Columbus, OH 43210 USA. EM anderson.2765@osu.edu OI Wright, Michelle/0000-0002-9348-8740 FU American Physiological Society; Robert Wood Johnson Foundation, Nurse Faculty Scholar Award [64202, 64193]; New Faculty Scholar Award, Senate Scholarly Activities Committee; University of North Dakota; University of North Dakota College of Nursing Office of Research; Sigma Theta Tau International FX The authors are grateful to the study participants, local health care facility and the Grand Forks Human Nutrition Center who made this study possible. We would like to acknowledge the contributions in data collection of Christine (Seames) Iseminger and Carole Wheeler, both supported by the American Physiological Society (Undergraduate Research Fellowship & Frontiers in Physiology, respectively) to this project. This project was supported by grants from the Robert Wood Johnson Foundation, Nurse Faculty Scholar Award (Anderson, #64202; Taylor, #64193), New Faculty Scholar Award, Senate Scholarly Activities Committee (Anderson), University of North Dakota, University of North Dakota College of Nursing Office of Research (Anderson), Sigma Theta Tau International (Anderson, #3781), and the American Physiological Society (Anderson). NR 62 TC 3 Z9 3 U1 0 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0024-3205 EI 1879-0631 J9 LIFE SCI JI Life Sci. PD MAY 15 PY 2015 VL 129 SI SI BP 10 EP 15 DI 10.1016/j.lfs.2014.07.012 PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA CH4LE UT WOS:000354004200003 PM 25050465 ER PT J AU Staudhammer, CL Escobedo, FJ Holt, N Young, LJ Brandeis, TJ Zipperer, W AF Staudhammer, Christina L. Escobedo, Francisco J. Holt, Nathan Young, Linda J. Brandeis, Thomas J. Zipperer, Wayne TI Predictors, spatial distribution, and occurrence of woody invasive plants in subtropical urban ecosystems SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Spatial analysis; Urban forest structure; Forest inventory and analysis; Socio-ecological systems; Predictive models ID PUERTO-RICO; NEW-ENGLAND; FOREST; USA; MANAGEMENT; DIVERSITY; RICHNESS; IMPACTS AB We examined the spatial distribution, occurrence, and socioecological predictors of woody invasive plants (WIP) in two subtropical, coastal urban ecosystems: San Juan, Puerto Rico and Miami-Dade, United States. These two cities have similar climates and ecosystems typical of subtropical regions but differ in socioeconomics, topography, and urbanization processes. Using permanent plot data, available forest inventory protocols and statistical analyses of geographic and socioeconomic spatial predictors, we found that landscape level distribution and occurrence of WIPs was not clustered. We also characterized WIP composition and occurrence using logistic models, and found they were strongly related to the proportional area of residential land uses. However, the magnitude and trend of increase depended on median household income and grass cover. In San Juan, WIP occurrence was higher in areas of high residential cover when incomes were low or grass cover was low, whereas the opposite was true in Miami-Dade. Although Miami-Dade had greater invasive shrub cover and numbers of WIP species, San Juan had far greater invasive tree density, basal area and crown cover. This study provides an approach for incorporating field and available census data in geospatial distribution models of WIPs in cities throughout the globe. Findings indicate that identifying spatial predictors of WIPs depends on site-specific factors and the ecological scale of the predictor. Thus, mapping protocols and policies to eradicate urban WIPs should target indicators of a relevant scale specific to the area of interest for their improved and proactive management. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Staudhammer, Christina L.] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35847 USA. [Escobedo, Francisco J.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Holt, Nathan; Young, Linda J.] Univ Florida, Dept Stat, Gainesville, FL 32611 USA. [Brandeis, Thomas J.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37919 USA. [Zipperer, Wayne] US Forest Serv, USDA, Southern Res Stn, Gainesville, FL 32611 USA. RP Escobedo, FJ (reprint author), Univ Florida, Sch Forest Resources & Conservat, 361 Newins Ziegler Hall,POB 110410, Gainesville, FL 32611 USA. EM cstaudhammer@ua.edu; fescobed@ufl.edu; nate.m.holt@gmail.com; ljyoung@ufl.edu; tjbrandeis@fs.fed.us; wzipperer@fs.fed.us RI Escobedo, Francisco /H-1286-2016 OI Escobedo, Francisco /0000-0002-9272-5046 FU USDA Tropical and Subtropical Agricultural Research Program [TSTAR-C FY2008] FX This research was funded by a grant from the USDA Tropical and Subtropical Agricultural Research Program (TSTAR-C FY2008). The authors would like to thank Joy Klein and Henry Mayer in Florida, and Robin Morgan, Terry Hoffman, Olga Ramos, Eileen Helmer, Jeffrey Glogiewicz and Edgardo Gonzalez in Puerto Rico for field logistics and data assistance, and Donald Hagan for his extremely helpful comments on an earlier draft of this manuscript. NR 48 TC 2 Z9 2 U1 2 U2 24 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAY 15 PY 2015 VL 155 BP 97 EP 105 DI 10.1016/j.jenvman.2015.03.012 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9CG UT WOS:000353611400011 PM 25776798 ER PT J AU Zheng, HQ Gong, HR Huang, SK Sohr, A Hu, FL Chen, YP AF Zheng, Huo-Qing Gong, Hong-Ri Huang, Shao-Kang Sohr, Alex Hu, Fu-Liang Chen, Yan Ping TI Evidence of the synergistic interaction of honey bee pathogens Nosema ceranae and Deformed wing virus SO VETERINARY MICROBIOLOGY LA English DT Article DE Apis mellifera; Nosema ceranae; Deformed wing virus; Synergistic effect; Pollen ID APIS-MELLIFERA-L.; COLONY COLLAPSE; MICROSPORIDIAN PARASITE; SPORE LOADS; INFECTION; LOSSES; APIARIES; WORKERS AB Nosema ceranae and Deformed wing virus (DWV) are two of the most prevalent pathogens currently attacking Western honey bees, Apis mellifera, and often simultaneously infect the same hosts. Here we investigated the effect of N. ceranae and Deformed wing virus (DWV) interactions on infected honey bees under lab conditions and at different nutrition statuses. Our results showed that Nosema could accelerate DWV replication in infected bees in a dose-dependent manner at the early stages of DWV infection. When bees were restricted from pollen nutrition, inoculation with 1 x 10(4) and 1 x 10(5) spores/bee could cause a significant increase in DWV titer, while inoculation with 1 x 10(3) spores/bee did not show any significant effect on the DWV titer. When bees were provided with pollen, only inoculation with 1 x 10(5) spores/bee showed significant effect on DWV titer. However, our results also showed that the two pathogens did not act synergistically when the titer of DWV reached a plateau. This study suggests that the synergistic effect of N. ceranae and DWV is dosage- and nutrition- dependent and that the synergistic interactions between the two pathogens could have implications on honey bee colony losses. Published by Elsevier B.V. C1 [Zheng, Huo-Qing; Gong, Hong-Ri; Hu, Fu-Liang] Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China. [Zheng, Huo-Qing; Huang, Shao-Kang; Sohr, Alex; Chen, Yan Ping] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Huang, Shao-Kang] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou, Fujian, Peoples R China. RP Chen, YP (reprint author), USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. EM judy.chen@ars.usda.gov FU Zhejiang Province Public Technology Research Program [2014C32060] FX We thank Michele Hamilton for her help in the lab. This research was supported by Zhejiang Province Public Technology Research Program (2014C32060). NR 39 TC 1 Z9 1 U1 1 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD MAY 15 PY 2015 VL 177 IS 1-2 BP 1 EP 6 DI 10.1016/j.vetmic.2015.02.003 PG 6 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CG9AW UT WOS:000353607800001 PM 25752367 ER PT J AU Pantin-Jackwood, MJ Costa-Hurtado, M Miller, PJ Afonso, CL Spackman, E Kapczynski, DR Shepherd, E Smith, D Swayne, DE AF Pantin-Jackwood, Mary J. Costa-Hurtado, Mar Miller, Patti J. Afonso, Claudio L. Spackman, Erica Kapczynski, Darrell R. Shepherd, Eric Smith, Diane Swayne, David E. TI Experimental co-infections of domestic ducks with a virulent Newcastle disease virus and low or highly pathogenic avian influenza viruses SO VETERINARY MICROBIOLOGY LA English DT Article DE Avian influenza virus; Newcastle disease virus; Domestic ducks; Co-infections; Viral interference ID EVALUATING VIRAL INTERFERENCE; INFECTIOUS-BRONCHITIS VIRUS; POLYMERASE CHAIN-REACTION; POULTRY; PARAMYXOVIRUSES; CHICKENS; SURVEILLANCE; WATERFOWL; STRAINS; TURKEYS AB Infections with avian influenza viruses (AIV) of low and high pathogenicity (LP and HP) and Newcastle disease virus (NDV) are commonly reported in domestic ducks in many parts of the world. However, it is not clear if co-infections with these viruses affect the severity of the diseases they produce, the amount of virus shed, and transmission of the viruses. In this study we infected domestic ducks with a virulent NDV virus (vNDV) and either a LPAIV or a HPAIV by giving the viruses individually, simultaneously, or sequentially two days apart. No clinical signs were observed in ducks infected or co-infected with vNDV and LPAIV, but co-infection decreased the number of ducks shedding vNDV and the amount of virus shed (P < 0.01) at 4 days post inoculation (dpi). Co-infection did not affect the number of birds shedding LPAIV, but more LPAIV was shed at 2 dpi (P < 0.0001) from ducks inoculated with only LPAIV compared to ducks co-infected with vNDV. Ducks that received the HPAIV with the vNDV simultaneously survived fewer days (P < 0.05) compared to the ducks that received the vNDV two days before the HPAIV. Co-infection also reduced transmission of vNDV to naive contact ducks housed with the inoculated ducks. In conclusion, domestic ducks can become co-infected with vNDV and LPAIV with no effect on clinical signs but with reduction of virus shedding and transmission. These findings indicate that infection with one virus can interfere with replication of another, modifying the pathogenesis and transmission of the viruses. Published by Elsevier B.V. C1 [Pantin-Jackwood, Mary J.; Costa-Hurtado, Mar; Miller, Patti J.; Afonso, Claudio L.; Spackman, Erica; Kapczynski, Darrell R.; Shepherd, Eric; Smith, Diane; Swayne, David E.] ARS, Exot & Emerging Avian Viral Dis Unit, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Pantin-Jackwood, MJ (reprint author), USDA, Southeast Poultry Res Lab, Agr Res Lab, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ARS.USDA.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU Agriculture Research Service CRIS Project [6612-320000-48]; National Institutes of Allergy and Infectious Diseases; National Institutes of Health Department of Health and Human Services [HHSN266200700007C] FX The authors appreciate the assistance provided by Aniko Zsak, Tim Olivier, Dawn Williams-Coplin, Rami Cha, Kira Moresco, Ronald Graham, and Roger Brock in conducting these studies. This work has been funded by the Agriculture Research Service CRIS Project 6612-320000-48 and with federal funds from the National Institutes of Allergy and Infectious Diseases, National Institutes of Health Department of Health and Human Services, under Contract No. HHSN266200700007C. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH. NR 47 TC 3 Z9 3 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD MAY 15 PY 2015 VL 177 IS 1-2 BP 7 EP 17 DI 10.1016/j.vetmic.2015.02.008 PG 11 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CG9AW UT WOS:000353607800002 PM 25759292 ER PT J AU Li, X Feng, G Sharratt, B Zheng, Z AF Li, X. Feng, G. Sharratt, B. Zheng, Z. TI Aerodynamic properties of agricultural and natural surfaces in northwestern Tarim Basin SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Cotton; Desert soils; Red date; Wind erosion; Zero plane displacement ID ZERO-PLANE DISPLACEMENT; THRESHOLD FRICTION VELOCITIES; ROUGHNESS LENGTH; CROP RESIDUES; WIND EROSION; VEGETATION; SOIL; HEIGHT; SEDIMENT; PROFILE AB Friction velocity (u*) and aerodynamic roughness (z(0)) are atmospheric parameters that influence the flux of windblown dust. These parameters have not been quantified for different land use types in the Tarim Basin of China, one of the largest sources of atmospheric dust in the world. Wind speed profiles were measured and used to determine d, u* and z(0) of a cotton (Gossypium hirsutum L.) field, red date (Ziziphus jujuba L) orchard and native desert during the spring wind erosion season of 2012 and 2013. In addition, d, u* and z(0) were estimated using Raupach's model. During periods of high winds sufficient to cause erosion in spring, d averaged 0.28, 0.067 and 0 m; u* averaged 0.39, 0.51 and 0.35 m s(-1); and z(0) averaged 7.0, 17.0 and 1.2 mm for respectively the cotton field, red date orchard and desert. Estimates of d, u* and z(0) according to Raupach's model during the same periods of high winds were 0.10, 0.16 and Urn; 0.56, 0.73 and 0.49 m s(-1); and 12.6, 21.9 and 1.8 mm for respectively the cotton field, red date orchard and desert. The Raupach model, which depends on canopy height and area index, overestimated u* and z(0) for the three land use types during most high wind events. The desert had the greatest potential for flux of windblown dust (due to the lowest zo) while the red date orchard has the lowest potential for dust flux (due to the highest zo). Published by Elsevier B.V. C1 [Li, X.; Feng, G.; Zheng, Z.] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Xinjiang, Peoples R China. [Sharratt, B.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Feng, G (reprint author), USDA ARS, POB 5367,810 Highway 12 East, Mississippi State, MS 39762 USA. EM Gary.Feng@ars.usda.gov FU National Natural Science Foundation of China [41171019]; One Hundred Talented Researchers Program of Chinese Academy of Sciences FX This research was supported by the National Natural Science Foundation of China (Grant No. 41171019), One Hundred Talented Researchers Program of Chinese Academy of Sciences. NR 40 TC 3 Z9 3 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 15 PY 2015 VL 204 BP 37 EP 45 DI 10.1016/j.agrformet.2015.01.005 PG 9 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CF0PN UT WOS:000352246800004 ER PT J AU Hoffman, CM Linn, R Parsons, R Sieg, C Winterkamp, J AF Hoffman, Chad M. Linn, Rodman Parsons, Russell Sieg, Carolyn Winterkamp, Judith TI Modeling spatial and temporal dynamics of wind flow and potential fire behavior following a mountain pine beetle outbreak in a lodgepole pine forest SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Turbulence; HIGRAD/FIRETEC; Fire behavior; Bark beetle; Heterogeneous fuels ID CROWN FIRE; TREE MORTALITY; CANOPY STRUCTURE; PROBABILITY; SIMULATION; PATTERNS; WILDFIRE; ATTACK; DISTURBANCES; TURBULENCE AB Patches of live, dead, and dying trees resulting from bark beetle-caused mortality alter spatial and temporal variability in the canopy and surface fuel complex through changes in the foliar moisture content of attacked trees and through the redistribution of canopy fuels. The resulting heterogeneous fuels complexes alter within-canopy wind flow, wind fluctuations, and rate of fire spread. However, there is currently little information about the potential influence of different rates and patterns of mortality on wind flow and fire behavior following bark beetle outbreaks. In this study, we contrasted withincanopy wind flow and fire rate-of-spread (ROS) at two different ambient wind speeds using FIRETEC for two differing bark beetle attack trajectories for a lodgepole pine (Pinus contorta) forest. These two attack trajectories represent different realizations of a bark beetle outbreak and result in different amounts and patterns of mortality through time. Our simulations suggested that the mean within-canopy wind velocities increased through time following the progression of mortality. In addition, we found that for a given level of mortality, a bark beetle outbreak that resulted in a higher degree of aggregation of canopy fuels had greater mean within-canopy wind velocities due to the channeling of wind flow. These findings suggest that bark beetle mortality can influence the mean within-canopy wind flow in two ways: first, by reducing the amount of vegetation present in the canopy acting as a source of drag; and second, by altering spatial patterns of vegetation that can lead to channeling of wind flow. Changes in the fire rate-of-spread were positively related to the level and continuity of bark beetle mortality. Peak rates of spread were between 1.2 and 2.7 times greater than the pre-outbreak scenario and coincided with a high level of mortality and minimal loss of canopy fuels. Following the loss of canopy fuels the rate of fire spread declined to levels below the initial phases of the outbreak in low wind speed cases but remained above pre-outbreak levels in high wind speed cases. These findings suggest that the rate and pattern of mortality arising from a bark beetle outbreak exerts significant influence on the magnitude and timing of alterations to the within-canopy wind flow and rate of fire spread. Our findings help clarify existing knowledge gaps related to the effect of bark beetle outbreaks on fire behavior and could explain potential differences in the reported effects of bark beetle outbreaks on fire behavior through time. (C) 2015 Elsevier B.V. All rights reserved. C1 [Hoffman, Chad M.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Linn, Rodman; Winterkamp, Judith] Los Alamos Natl Lab, Div Earth & Environm Sci, Los Alamos, NM 87544 USA. [Parsons, Russell] USDA, Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59802 USA. [Sieg, Carolyn] USDA, Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Hoffman, CM (reprint author), Colorado State Univ, Dept Forest & Rangeland Stewardship, 1472 Campus Delivery, Ft Collins, CO 80523 USA. EM c.hoffman@colostate.edu; rrl@lanl.gov; rparsons@fs.fed.us; csieg@fs.fed.us; judyw@lanl.gov OI Hoffman, Chad/0000-0001-8715-937X FU USDA Forest Service Research (Rocky Mountain Research Station and Washington office), National Fire Plan Dollars [11-JV-11221633-207]; Colorado State University; Los Alamos National Laboratory [09-IA-11221633-215, 13-IA-11221633-103] FX This research was funded by the USDA Forest Service Research (both Rocky Mountain Research Station and Washington office), National Fire Plan Dollars through Research Joint Venture Agreement 11-JV-11221633-207 with Colorado State University, and Interagency Agreements09-IA-11221633-215 and 13-IA-11221633-103 with Los Alamos National Laboratory. Los Alamos National Laboratory's Institutional Computing Program provided computational resources to complete this project. NR 49 TC 6 Z9 6 U1 3 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 15 PY 2015 VL 204 BP 79 EP 93 DI 10.1016/j.agrformet.2015.01.018 PG 15 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CF0PN UT WOS:000352246800009 ER PT J AU Huang, JX Tian, LY Liang, SL Ma, HY Becker-Reshef, I Huang, YB Su, W Zhang, XD Zhu, DH Wu, WB AF Huang, Jianxi Tian, Liyan Liang, Shunlin Ma, Hongyuan Becker-Reshef, Inbal Huang, Yanbo Su, Wei Zhang, Xiaodong Zhu, Dehai Wu, Wenbin TI Improving winter wheat yield estimation by assimilation of the leaf area index from Landsat TM and MODIS data into the WOFOST model SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Data assimilation; Leaf area index; Scale adjustment; Wheat yield estimation; WOFOST ID CROP GROWTH-MODEL; REMOTELY-SENSED DATA; CERES-MAIZE MODEL; TIME-SERIES; REGIONAL-SCALE; SENSING DATA; NORTH CHINA; FIELD; LAI; INFORMATION AB To predict regional-scale winter wheat yield, we developed a crop model and data assimilation framework that assimilated leaf area index (LAI) derived from Landsat TM and MODIS data into the WOFOST crop growth model. We measured LAI during seven phenological phases in two agricultural cities in China's Hebei Province. To reduce cloud contamination, we applied Savitzky-Golay (S-G) filtering to the MODIS LAI products to obtain a filtered LAI. We then regressed field-measured LAI on Landsat TM vegetation indices to derive multi-temporal TM LAIs. We developed a nonlinear method to adjust LAI by accounting for the scale mismatch between the remotely sensed data and the model's state variables. The TM LAI and scale-adjusted LAI datasets were assimilated into the WOFOST model to allow evaluation of the yield estimation accuracy. We constructed a four-dimensional variational data assimilation (4DVar) cost function to account for the observations and model errors during key phenological stages. We used the shuffled complex evolution-University of Arizona algorithm to minimize the 4DVar cost function between the remotely sensed and modeled LAI and to optimize two important WOFOST parameters. Finally, we simulated winter wheat yield in a 1-km grid for cells with at least 50% of their area occupied by winter wheat using the optimized WOFOST, and aggregated the results at a regional scale. The scale adjustment substantially improved the accuracy of regional wheat yield predictions (R-2=0.48; RMSE = 151.92 kg ha(-1)) compared with the unassimilated results (R-2 = 0.23; RMSE=373.6 kg ha(-1)) and the TM LAI results (R-2=0.27; RMSE=191.6 kg ha(-1)). Thus, the assimilation performance depends strongly on the LAI retrieval accuracy and the scaling correction. Our research provides a scheme to employ remotely sensed data, ground-measured data, and a crop growth model to improve regional crop yield estimates. (C) 2015 Elsevier B.V. All rights reserved. C1 [Huang, Jianxi; Tian, Liyan; Ma, Hongyuan; Su, Wei; Zhang, Xiaodong; Zhu, Dehai] China Agr Univ, Coll Informat & Elect Engn, Beijing 100083, Peoples R China. [Liang, Shunlin] Beijing Normal Univ, Sch Geog, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. [Liang, Shunlin; Becker-Reshef, Inbal] Univ Maryland, Dept Geog Sci, College Pk, MD 20740 USA. [Huang, Yanbo] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS USA. [Wu, Wenbin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. RP Huang, JX (reprint author), China Agr Univ, Coll Informat & Elect Engn, 17 Qjnghua East Rd, Beijing 100083, Peoples R China. EM jxhuang@cau.edu.cn RI liang, shunlin/C-2809-2015; OI Huang, Jianxi/0000-0003-0341-1983 FU National Natural Science Foundation Project of China [41371326] FX This study was financially supported by the National Natural Science Foundation Project of China (No. 41371326), the authors are grateful to the anonymous reviewers who provided constructive comments and suggestions to improve this manuscript. NR 42 TC 25 Z9 29 U1 6 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 15 PY 2015 VL 204 BP 106 EP 121 DI 10.1016/j.agrformet.2015.02.001 PG 16 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA CF0PN UT WOS:000352246800011 ER PT J AU Gonzalez-Benecke, CA Samuelson, LJ Stokes, TA Cropper, WP Martin, TA Johnsen, KH AF Gonzalez-Benecke, C. A. Samuelson, L. J. Stokes, T. A. Cropper, W. P., Jr. Martin, T. A. Johnsen, K. H. TI Understory plant biomass dynamics of prescribed burned Pinus palustris stands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Longleaf pine; Ground cover; Fire management; Restoration ecology; Modeling ID MIXED HARDWOOD FOREST; LONGLEAF PINE; HERBACEOUS VEGETATION; CARBON DYNAMICS; GROWING SEASONS; SOUTH-CAROLINA; COASTAL-PLAIN; NORTH FLORIDA; FIRE; ECOSYSTEM AB Longleaf pine (Pinus palustris Mill.) forests are characterized by unusually high understory plant species diversity, but models describing understory ground cover biomass, and hence fuel load dynamics, are scarce for this fire-dependent ecosystem. Only coarse scale estimates, being restricted on accuracy and geographical extrapolation, are available. We analyzed the dynamics of ground cover biomass under different prescribed burning regimes in longleaf pine stands in the southeastern United States. We developed a set of functions to simulate ground cover biomass dynamics in stands of varying age, basal area and fire management history. The subsequent models allow for estimation of ground cover biomass for unburned stands and living woody and herbaceous ground cover biomass for burned stands. Woody ground cover was highly reduced as fire frequency increased, and also affected by stand basal area when time since last burning was longer than two years. Herbaceous ground cover was affected little by burning frequency but was reduced as basal area increased. This novel model system is a useful tool that can be incorporated into fire management and carbon balance models. (C) 2015 Elsevier B.V. All rights reserved. C1 [Gonzalez-Benecke, C. A.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Samuelson, L. J.; Stokes, T. A.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Cropper, W. P., Jr.; Martin, T. A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Johnsen, K. H.] US Forest Serv, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Gonzalez-Benecke, CA (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97331 USA. EM cgonzabe@ufl.edu RI Cropper, Jr., Wendell/E-5952-2010; OI Cropper, Jr., Wendell/0000-0001-7851-7382; Martin, Timothy/0000-0002-7872-4194 FU U.S. Department of Defense through the Strategic Environmental Research and Development Program (SERDP); USDA Forest Service [11330123-147] FX This research was supported by the U.S. Department of Defense through the Strategic Environmental Research and Development Program (SERDP) and the USDA Forest Service Grant No. 11330123-147. NR 72 TC 4 Z9 4 U1 12 U2 79 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2015 VL 344 BP 84 EP 94 DI 10.1016/j.foreco.2015.02.018 PG 11 WC Forestry SC Forestry GA CE6US UT WOS:000351974300009 ER PT J AU Vogan, PJ Schoettle, AW AF Vogan, Patrick J. Schoettle, Anna W. TI Selection for resistance to white pine blister rust affects the abiotic stress tolerances of limber pine SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Cronartium ribiola; Pinus flexilis; Cold hardiness; Drought; Disease resistance; Restoration ID DOUGLAS-FIR SEEDLINGS; COLD-HARDINESS; CHLOROPHYLL FLUORESCENCE; CRONARTIUM-RIBICOLA; SUGAR PINE; ANTIFREEZE PROTEINS; NATURAL-POPULATIONS; NEEDLE MORPHOLOGY; DROUGHT STRESS; WATER-STRESS AB Limber pine (Pinus flexilis) mortality is increasing across the West as a result of the combined stresses of white pine blister rust (Cronartium ribicola; WPBR), mountain pine beetle (Dendroctonus ponderosae), and dwarf mistletoe (Arceuthobium cyanocarpum) in a changing climate. With the continued spread of WPBR, extensive mortality will continue with strong selection against trees that lack genetic resistance to the disease. Naturally-occurring resistance to the non-native fungal pathogen Cronartium ribicola is present in limber pine and is the cornerstone of restoration strategies. Disease resistance to native pathogens can carry a strong fitness cost to the host in the absence of the pathogen. However we suspect this to be unlikely in the case of resistance to a non-native pathogen as the resistance would not have persisted in the pre-invasion population. Genetic resistance to a novel stress which the species has not co-evolved may be neutral or carry a benefit to the host via a function that offers adaptive benefit for environmental factor(s), biotic or abiotic, under which it did evolve. Because plant disease defenses can share physiological activity with those that mediate freezing and drought stress sensitivity, both stresses for which limber pine is responsive, we compared cold and drought tolerances of limber pine seedling families from trees previously determined to have (R families) and not have (S families) the Cr4 allele for qualitative resistance to WPBR. R families constitutively had (1) greater cold hardiness than S families and (2) lower stomatal conductance than S families during moderate drought, suggesting that R and S families have different abiotic stress responses such that the post-invasion populations may have the potential for modified competitive ability, especially under a warming climate. The presence of different stress tolerances in R families may also inform hypotheses to explain the presence and frequency of a resistance gene against a non-native pathogen to which the species was not previously exposed. We conclude that as the frequency of qualitative resistance to WPBR increases, through natural selection or planting of disease resistant seedling stock, the resultant populations may have a different suite of stress tolerance traits than pre-invasion populations. The shift in the fundamental niche of limber pine in the presence of Cronartium ribicola should be considered when selecting seed sources and habitats for restoration and projecting future distributions in a changing climate. Published by Elsevier B.V. C1 [Vogan, Patrick J.] Mt Studies Inst, Silverton, CO 81433 USA. [Vogan, Patrick J.; Schoettle, Anna W.] USDA, Rocky Mt Res Stn, Forest Serv, Ft Collins, CO 80256 USA. RP Schoettle, AW (reprint author), USDA, Rocky Mt Res Stn, Forest Serv, 240 West Prospect Rd, Ft Collins, CO 80256 USA. EM voganpj@yahoo.com; aschoettle@fs.fed.us FU USDA Forest Service's Climate Change Program; Genetic Conservation Program; Special Technology Development Program FX We extend special thanks to Lisa Winn and Richard Sniezko for welcoming us to conduct this work at Dorena Genetic Resource Center and to Lee Riley, Angelia Kegley and the Dorena Nursery and Rust staffs for excellent seedling care and inoculation, and Doug Savin for statistical consultation. We also thank Glenn Howe, Keith JS Jayawickrama, and Lauren Magalska of Oregon State University, Brad St. Clair of USDA Forest Service Pacific Northwest Research Station, and Silvia L'Hirondelle of the Research branch of the British Columbia Government (Canada) for advice and assistance with the cold hardiness testing, Christy Cleaver for graphics support, and Mountain Studies Institute (Silverton, CO) for administrative support. We appreciate the anonymous reviewers who helped to improve this manuscript. Funding was provided in part by grant awards to AWS from USDA Forest Service's Climate Change Program, Genetic Conservation Program and Special Technology Development Program. NR 69 TC 4 Z9 4 U1 6 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2015 VL 344 BP 110 EP 119 DI 10.1016/j.foreco.2015.01.029 PG 10 WC Forestry SC Forestry GA CE6US UT WOS:000351974300011 ER PT J AU Fan, XT AF Fan, Xuetong TI Furan formation from fatty acids as a result of storage, gamma irradiation, UV-C and heat treatments SO FOOD CHEMISTRY LA English DT Article DE Furan; Fatty acid; Thermal; UV; Ionizing radiation; Linoleic acid; Linolenic acid ID IN-WATER EMULSIONS; ASCORBIC-ACID; MODEL SYSTEMS; BULK OILS; ANTIOXIDANT ACTIVITY; LIPID-PEROXIDATION; ALPHA-TOCOPHEROL; HEADSPACE METHOD; PROCESSED FOODS; LINOLEIC-ACID AB The effects of gamma and UV-C irradiation in comparison with thermal processing and storage at 25 degrees C on formation of furan from different fatty acids were investigated. Results showed that furan was generated from polyunsaturated fatty acids such as linoleic and linolenic acid during thermal (120 degrees C, 25 min) and UV-C (11.5 J/cm(2)) treatments. Gamma irradiation (up to 20 kGy) did not induce formation of significant amounts of furan from any of the fatty acids studied. Storage of unsaturated fatty acid emulsions at 25 degrees C for 3 days led to the formation of furan (7-11 ng/mL) even without prior thermal or non-thermal treatments. pH significantly impacted furan formation with >3.5 times more furan formed at pH 9 than at pHs 3 or 6 during 3 days at 25 degrees C. The addition of Trolox, BHA, and propyl gallate had no significant effect on furan formation from linolenic acid while alpha-tocopherol and FeSO4 promoted furan formation. Published by Elsevier Ltd. C1 ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov NR 42 TC 4 Z9 4 U1 9 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAY 15 PY 2015 VL 175 BP 439 EP 444 DI 10.1016/j.foodchem.2014.12.002 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CA4ML UT WOS:000348878500061 PM 25577103 ER PT J AU O'Neil, CE Nicklas, TA Fulgoni, VL AF O'Neil, Carol E. Nicklas, Theresa A. Fulgoni, Victor L., III TI Consumption of apples is associated with a better diet quality and reduced risk of obesity in children: National Health and Nutrition Examination Survey (NHANES) 2003-2010 SO NUTRITION JOURNAL LA English DT Article DE NHANES; Apple; Apple juice; Apple sauce; Children; Fruit; Weight; Diet quality ID IMPROVED NUTRIENT ADEQUACY; CORONARY-HEART-DISEASE; VEGETABLE INTAKE; JUICE CONSUMPTION; ENERGY-INTAKE; PRESCHOOL-CHILDREN; FRUIT CONSUMPTION; BODY-WEIGHT; METAANALYSIS; COHORT AB Background: Most children do not meet the recommendation for fruit consumption. Apples are the second most commonly consumed fruit in the US; however, no studies have examined the association of total apple products, apples, apple sauce, and 100 % apple juice consumption on diet quality and weight/adiposity in children. Methods: The purpose of this study was to examine the association between various apple consumption forms with diet quality and weight/adiposity in a nationally representative sample of children. Participants were children 2-18 years of age (N = 13,339) from the National Health and Nutrition Examination Survey 2003-2010. Intake was determined using a single interview administered 24-h diet recall. Apple product consumption was determined using the cycle-appropriate USDA Food and Nutrient Database for Dietary Studies food codes. Total diet quality and component scores were determined using the Healthy Eating Index-2010 (HEI). Anthropometrics were determined using standard methods. Covariate adjusted linear and logistic regressions were used to compare apple product consumers with non-consumers; sample weights were used. Probability was set at <0.01. Results: Approximately 26 % of the population (n = 3,482) consumed some form of apple products. Consumers of apple products, whole apples, apple sauce, and 100 % apple juice had higher HEI scores than non-consumers: 50.4 +/- 0.4 v 41.9 +/- 0.3, 52.5 +/- 0.5 v 42.7 +/- 0.3, 52.1 +/- 0.8 v 47.2 +/- 0.4, and 51.4 +/- 0.6 v 46.5 +/- 0.4, respectively. Apple products and whole apple consumers had lower BMI z-scores than non-consumers: 0.4 +/- 0.04 v 0.5 +/- 0.03 and 0.3 +/- 0.1 v 0.5 +/- 0.02, respectively. Apple products and whole apple consumers were 25 % (0.59-0.95 99th CI) and 30 % (0.52-0.95 99th CI), respectively, were less likely to be obese than non-consumers. Conclusions: Consumption of any form of apples contributed to the fruit recommendation of children and improved diet quality. Apples should be included in the diets of children as a component of an overall healthy diet. C1 [O'Neil, Carol E.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Nicklas, Theresa A.] USDA ARS, Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Fulgoni, Victor L., III] Nutr Impact LLC, Battle Creek, MI 49014 USA. RP O'Neil, CE (reprint author), Louisiana State Univ, Ctr Agr, 261 Knapp Hall, Baton Rouge, LA 70803 USA. EM coneil1@lsu.edu FU United States Department of Agriculture/Agricultural Research Service (USDA/ARS) [58-3092-5-001]; USDA Hatch Project [LAB 94209]; Dr. Pepper/Snapple FX This work is a publication of the USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. Partial support was received from the United States Department of Agriculture/Agricultural Research Service (USDA/ARS) through specific cooperative agreement 58-3092-5-001 and from the USDA Hatch Project LAB 94209. Partial support was also received by the Dr. Pepper/Snapple. The funding agencies had no input into the study design or interpretation of the data. The authors declare that they have no conflict regarding this paper and have no involvements that might raise the question of bias in the work reported or in the conclusions, implications, and opinions stated. All authors have no involvements that might raise the question of bias in the work reported or in the conclusions, implications, and opinions stated. NR 60 TC 4 Z9 4 U1 4 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2891 J9 NUTR J JI Nutr. J. PD MAY 14 PY 2015 VL 14 AR 48 DI 10.1186/s12937-015-0040-1 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CI9DZ UT WOS:000355071900001 PM 25971247 ER PT J AU Song, ZW Schlatter, D Kennedy, P Kinkel, LL Kistler, HC Nguyen, N Bates, ST AF Song, Zewei Schlatter, Dan Kennedy, Peter Kinkel, Linda L. Kistler, H. Corby Nhu Nguyen Bates, Scott T. TI Effort versus Reward: Preparing Samples for Fungal Community Characterization in High-Throughput Sequencing Surveys of Soils SO PLOS ONE LA English DT Article ID REAL-TIME PCR; GEL-ELECTROPHORESIS ANALYSIS; DNA EXTRACTION METHODS; ENVIRONMENTAL DNA; MYCORRHIZAL FUNGI; DIVERSITY; PURIFICATION; GRADIENT; PRODUCTIVITY; BIODIVERSITY AB Next generation fungal amplicon sequencing is being used with increasing frequency to study fungal diversity in various ecosystems; however, the influence of sample preparation on the characterization of fungal community is poorly understood. We investigated the effects of four procedural modifications to library preparation for high-throughput sequencing (HTS). The following treatments were considered: 1) the amount of soil used in DNA extraction, 2) the inclusion of additional steps (freeze/thaw cycles, sonication, or hot water bath incubation) in the extraction procedure, 3) the amount of DNA template used in PCR, and 4) the effect of sample pooling, either physically or computationally. Soils from two different ecosystems in Minnesota, USA, one prairie and one forest site, were used to assess the generality of our results. The first three treatments did not significantly influence observed fungal OTU richness or community structure at either site. Physical pooling captured more OTU richness compared to individual samples, but total OTU richness at each site was highest when individual samples were computationally combined. We conclude that standard extraction kit protocols are well optimized for fungal HTS surveys, but because sample pooling can significantly influence OTU richness estimates, it is important to carefully consider the study aims when planning sampling procedures. C1 [Song, Zewei; Schlatter, Dan; Kinkel, Linda L.; Kistler, H. Corby; Bates, Scott T.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Kennedy, Peter; Nhu Nguyen] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. [Kistler, H. Corby] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Song, ZW (reprint author), Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. EM songx208@umn.edu FU United States Department of Agriculture grant [2011-67019-30200]; University of Minnesota MnDrive grant; National Science Foundation [EF 12-41895]; National Science Foundation (Long-Term Ecological Research Network) [0620652] FX Funding was received from United States Department of Agriculture (2011-67019-30200 http:// nifa.usda.gov/grants) grant to LLK & HCK; University of Minnesota MnDrive (http://mndrive.umn.edu/) grant to LLK & STB; and National Science Foundation (Grants EF 12-41895 grant to LLK and Long-Term Ecological Research Network 0620652). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 4 Z9 4 U1 3 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 14 PY 2015 VL 10 IS 5 AR UNSP e0127234 DI 10.1371/journal.pone.0127234 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI2AC UT WOS:000354545600103 PM 25974078 ER PT J AU Yao, JL Kula, RR Wharton, RA Chen, JH AF Yao, Junli Kula, Robert R. Wharton, Robert A. Chen, Jiahua TI Four new species of Tanycarpa (Hymenoptera, Braconidae, Alysiinae) from the Palaearctic Region and new records of species from China SO ZOOTAXA LA English DT Article DE Alysiini; Asia; identification; key; range extension; taxonomy ID REVISION AB Four new species of Tanycarpa (Hymenoptera, Braconidae, Alysiinae), T. gymnonotum Yao sp. n., T. similis Yao sp. n., T. areolata Yao sp. n., and T. lineata Yao sp. n., are described from the Palaearctic Region of China, and T. chors Belokobylskij is newly recorded from China. Significant range extensions are given for T. bicolor (Nees von Esenbeck), T. gracilicornis (Nees von Esenbeck), and T. mitis Stelfox. A key to the Palaearctic species of Tanycarpa is provided. C1 [Yao, Junli; Chen, Jiahua] Fujian Agr & Forestry Univ, Beneficial Insects Inst, Fuzhou 350002, Fujian, Peoples R China. [Kula, Robert R.] ARS, Systemat Entomol Lab, Beltsville Agr Res Ctr, USDA,Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. [Wharton, Robert A.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. RP Chen, JH (reprint author), Fujian Agr & Forestry Univ, Beneficial Insects Inst, Fuzhou 350002, Fujian, Peoples R China. EM yao.junli.china@gmail.com; Robert.Kula@ars.usda.gov; rawbaw2@tamu.edu; JhChen34@163.com FU Specialized Research Fund for the Doctoral Program in Colleges and Universities for the project Systemic Studies on Alysiinae of China [20113515110003] FX The images in this paper were acquired by the first author with the help of Andrew Ly and Danielle Restuccia while the first author was visiting Texas A&M University. We thank Dr. Dicky Yu for providing many references. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. This research was supported by the Specialized Research Fund for the Doctoral Program in Colleges and Universities (2011) for the project Systemic Studies on Alysiinae of China (code: 20113515110003). NR 30 TC 0 Z9 0 U1 0 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAY 14 PY 2015 VL 3957 IS 2 BP 169 EP 187 PG 19 WC Zoology SC Zoology GA CI8BV UT WOS:000354993400002 PM 26249064 ER PT J AU Lehotay, SJ Riter, LS Saha, M AF Lehotay, Steven J. Riter, Leah S. Saha, Manasi TI Residues in Food and Feed Topic Area at the 13th IUPAC International Congress of Pesticide Chemistry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article AB The organizers of two symposia in the "Residues in Food and Feed" topic area held at the 13th IUPAC, International Congress Of Pesticide Chemistry introduce the papers that were contributed to this special Section in the Journal. The Symposia were titled "Taking Advantage of Advanced Analytical Tools" and "Going from Macro to Micro: The Future of Sample Processing in Residue Analytical Methods". The oral and poster Sessions generated much interest and discussion among the attendees, and some highlights are described in this introductory paper. C1 [Lehotay, Steven J.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Riter, Leah S.] Monsanto Co, Environm Sci Technol Ctr, St Louis, MO 63167 USA. [Saha, Manasi] BASF Crop Protect, Consumer & Environm Safety, Res Triangle Pk, NC 27709 USA. RP Lehotay, SJ (reprint author), USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM Steven.Lehotay@ars.usda.gov NR 0 TC 1 Z9 1 U1 2 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 13 PY 2015 VL 63 IS 18 BP 4393 EP 4394 DI 10.1021/jf5059599 PG 2 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CI6XZ UT WOS:000354908800001 PM 25660997 ER PT J AU Lehotay, SJ Cook, JM AF Lehotay, Steven J. Cook, Jo Marie TI Sampling and Sample Processing in Pesticide Residue Analysis SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE sampling; sample comminution; processing; homogenization; blending; milling; grinding; theoly of sampling; high-throughput analysis; pesticide residues; cryomilling; validation; food; soil ID MEASUREMENT UNCERTAINTY; MULTIRESIDUE ANALYSIS; CROP UNITS; VEGETABLES; VARIABILITY; FRUITS; FOODS; EXTRACTION; STABILITY; AGRICULTURE AB Proper sampling-and sample processing in pesticide residue analysis of food and sail have always been essential to obtain accurate results, but the subject is becoming a greater concern as approximately 100 mg test portions are being analyzed with automated high-throughput analytical methods by agrochemical industry and contract laboratories. As global food trade and the importance of monitoring increase, the food industry and regulatory laboratories are also considering miniaturized high-throughput methods. In conjunction with a summary of the symposium "Residues in Food and Feed - Going from Macro to Micro: The Future of Sample Processing in Residue Analytical Methods held at the 13th IUPAC International Congress of Pesticide Chemistry, this is an Opportune time to review sampling theory and sample processing for pesticide residue analysis. If; collected samples and test portions do not adequately represent the actual lot from which they came and provide meaningful results, then all costs, nine, and efforts involved in implementing programs using sophisticated analytical instruments and techniques are Wasted and can actually yield misleading results. This paper is designed to briefly review the often-neglected but crucial topic of sample collection and processing and put the issue into perspective for the future of pesticide residue analysis. It also emphasizes that analysts should demonstrate the validity of their sample processing approaches for the analytes/matrices of interest and encourages further studies on sampling and sample mass reduction to produce a test portion. C1 [Lehotay, Steven J.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Cook, Jo Marie] Florida Dept Agr & Consumer Serv, Div Food Safety, Tallahassee, FL 32399 USA. RP Cook, JM (reprint author), Florida Dept Agr & Consumer Serv, Div Food Safety, 3125 Conner Blvd, Tallahassee, FL 32399 USA. EM JoMarie.Cook@freshfromflorida.com NR 53 TC 6 Z9 6 U1 1 U2 31 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 13 PY 2015 VL 63 IS 18 BP 4395 EP 4404 DI 10.1021/jf5056985 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CI6XZ UT WOS:000354908800002 PM 25677085 ER PT J AU Cheseto, X Kuate, SP Tchouassi, DP Ndung'u, M Teal, PEA Torto, B AF Cheseto, Xavier Kuate, Serge Philibert Tchouassi, David P. Ndung'u, Mary Teal, Peter E. A. Torto, Baldwyn TI Potential of the Desert Locust Schistocerca gregaria (Orthoptera: Acrididae) as an Unconventional Source of Dietary and Therapeutic Sterols SO PLOS ONE LA English DT Article ID PLANT STEROLS; BIOSYNTHESIS; INSECTS; METABOLISM; NUTRITION; PHYTOSTEROLS; GRASSHOPPER; MECHANISMS; ECDYSONE; ENZYMES AB Insects are increasingly being recognized not only as a source of food to feed the ever growing world population but also as potential sources of new products and therapeutic agents, among which are sterols. In this study, we sought to profile sterols and their derivatives present in the desert locust, Schistocerca gregaria, focusing on those with potential importance as dietary and therapeutic components for humans. Using coupled gas chromatography-mass spectrometry (GC-MS), we analyzed and compared the quantities of sterols in the different sections of the gut and tissues of the locust. In the gut, we identified 34 sterols which showed a patchy distribution, but with the highest composition in the foregut (55%) followed by midgut (31%) and hindgut (14%). Fed ad libitum on wheat seedlings, five sterols unique to the insect were detected. These sterols were identified as 7-dehydrocholesterol, desmosterol, fucosterol, (3 beta, 5 alpha) cholesta-8, 14, 24-trien-3-ol, 4, 4-dimethyl, and (3 beta, 20R) cholesta-5, 24-dien-3, 20-diol with the first three having known health benefits in humans. Incubation of the fore-, mid- and hindgut with cholesterol-[4-C-13] yielded eight derivatives, three of these were detected in the gut of the desert locust after it had consumed the vegetative diet but were not detected in the diet. Our study shows that the desert locust ingests phytosterols from a vegetative diet and, amplifies and metabolizes them into derivatives with potential salutary benefits and we discuss our findings in this context. C1 [Cheseto, Xavier; Kuate, Serge Philibert; Tchouassi, David P.; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol Icipe, Behav & Chem Ecol Dept, Nairobi, Kenya. [Cheseto, Xavier; Ndung'u, Mary] Jomo Kenyatta Univ Agr & Technol, Dept Chem, Nairobi, Kenya. [Teal, Peter E. A.] USDA ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Torto, B (reprint author), Int Ctr Insect Physiol & Ecol Icipe, Behav & Chem Ecol Dept, Nairobi, Kenya. EM btorto@icipe.org FU icipe-Dissertation Research Internship Program (DRIP); USDA/ARS-CMAVE FX Support from icipe-Dissertation Research Internship Program (DRIP) and USDA/ARS-CMAVE is gratefully acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 2 Z9 3 U1 2 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 13 PY 2015 VL 10 IS 5 AR e0127171 DI 10.1371/journal.pone.0127171 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI1ZP UT WOS:000354544200154 PM 25970517 ER PT J AU Hou, L Chen, XM Wang, MN See, DR Chao, SM Bulli, P Jing, JX AF Hou, Lu Chen, Xianming Wang, Meinan See, Deven R. Chao, Shiaoman Bulli, Peter Jing, Jinxue TI Mapping a Large Number of QTL for Durable Resistance to Stripe Rust in Winter Wheat Druchamp Using SSR and SNP Markers SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCI; ADULT-PLANT RESISTANCE; F-SP TRITICI; ALL-STAGE RESISTANCE; NORTH-AMERICAN RACES; PUCCINIA-STRIIFORMIS; HIGH-TEMPERATURE; BREAD WHEAT; UNITED-STATES; YELLOW RUST AB Winter wheat Druchamp has both high-temperature adult-plant (HTAP) resistance and all-stage resistance to stripe rust caused by Puccinia striiformisf. sp. tritici (Pst). The HTAP resistance in Druchamp is durable as the variety has been resistant in adult-plant stage since it was introduced from France to the United States in late 1940s. To map the quantitative trait loci (QTL) for stripe rust resistance, an F8 recombinant inbred line (RIL) population from cross Druchamp x Michigan Amber was phenotyped for stripe rust response in multiple years in fields under natural infection and with selected Pst races under controlled greenhouse conditions, and genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers. Composite interval mapping (CIM) identified eight HTAP resistance QTL and three all-stage resistance QTL. Among the eight HTAP resistance QTL, QYrdr.wgp-1BL.2 (explaining 2.36-31.04% variation), QYrdr.wgp-2BL (2.81-15.65%), QYrdr.wgp-5AL(2.27-17.22%) and QYrdr.wgp-5BL.2 (2.42-15.13%) were significant in all tests; and QYrdr.wgp-1BL.1 (1.94-10.19%), QYrdr.wgp-1DS (2.04-27.24%), QYrdr.wgp-3AL (1.78-13.85%) and QYrdr.wgp-6BL. 2 (1.69-33.71%) were significant in some of the tests. The three all-stage resistance QTL, QYrdr.wgp-5BL.1 (5.47-36.04%), QYrdr.wgp-5DL (9.27-11.94%) and QYrdr.wgp-6BL.1(13.07-20.36%), were detected based on reactions in the seedlings tested with certain Pstraces. Among the eleven QTL detected in Druchamp, at least three (QYrdr.wgp-5DL for race-specific all-stage resistance and QYrdr.wgp-3AL and QYrdr.wgp-6BL.2 for race non-specific HTAP resistance) are new. All these QTL, especially those for durable HTAP resistance, and their closely linked molecular markers could be useful for developing wheat cultivars with durable resistance to stripe rust. C1 [Hou, Lu] Qinghai Acad Agr & Forestry Sci, Inst Plant Protect, Key Lab Agr Integrated Pest Management, Xining, Qinghai, Peoples R China. [Hou, Lu; Chen, Xianming; Wang, Meinan; See, Deven R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Hou, Lu; Jing, Jinxue] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Hou, Lu; Jing, Jinxue] Northwest A&F Univ, Coll Plant Protect, Yangling, Shaanxi, Peoples R China. [Chen, Xianming; See, Deven R.] USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res, Fargo, ND USA. [Bulli, Peter] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Chen, XM (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM xianming@wsu.edu FU US Department of Agriculture, Agricultural Research Service [5348-22000-015-00D]; Washington State University, PPNS [13C-3061-5665, 13Z-3061-6665, 0670]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH, Washington State University, Pullman, WA, USA [WNP00663]; Northwest A;F University Plant Pathology; "973" project [2013CB127700]; "111" Project [B07049]; China Scholarship Council scholarship FX This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-015-00D) and Washington State University (Project No. 13C-3061-5665 and 13Z-3061-6665) PPNS No. 0670, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH Project Number WNP00663, Washington State University, Pullman, WA, 99164-6430, USA. The research is also part of the Northwest A &;F University Plant Pathology, the "973" project (2013CB127700) and "111" Project (B07049). The China Scholarship Council scholarship to the first author is greatly appreciated. NR 70 TC 3 Z9 3 U1 5 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 13 PY 2015 VL 10 IS 5 AR UNSP e0126794 DI 10.1371/journal.pone.0126794 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI1ZP UT WOS:000354544200131 PM 25970329 ER PT J AU Glenn, DM Bassett, CB Tworkoski, T Scorza, R Miller, SS AF Glenn, D. M. Bassett, C. B. Tworkoski, T. Scorza, R. Miller, S. S. TI Tree architecture of pillar and standard peach affect canopy transpiration and water use efficiency SO SCIENTIA HORTICULTURAE LA English DT Article DE Prunus persica; Leaf area index; Whole plant gas exchange; Light interception ID LIGHT INTERCEPTION; GROWTH TYPES; PHOTOSYNTHESIS; CULTIVARS; SYSTEMS; CROWN; LEAF AB The development of productive high density peach orchards is often limited by the excessive vegetative growth of the trees that reduces productivity and quality. Dwarfing rootstocks are not available but upright tree architectures have been developed for high density peach production. The purpose of this study was to determine the water use efficiency of upright pillar and standard architecture peach trees in a production setting in order to understand the factors that differentiate transpiration and water use efficiency of the two tree architectures. There were no differences in leaf area index (LAI) of the pillar and standard types but the standards architecture had significantly greater leaf area density (LAD) than the pillar. There was no significant difference in the photosynthetically active radiation (PAR) response of photosynthesis (A) and transpiration (E) for the pillar and standard trees over a range of LAI's and vapor pressure deficits (VPD) for the 3 year period indicating that there was no genetic difference in gas exchange mechanisms between the genotypes of the two tree architectures. For high light conditions, the pillar and standard trees had a similar and non-significantly different E: VPD relationship for LAI < 2.25, however, for LAI > 2.25, the pillar had a significantly (P=0.05) greater E than the standard architecture at similar VPD levels due to the decreased LAD of the pillar architecture that more effectively distributed light within the canopy. The increased illumination and interception of PAR by the pillar canopy increased both photosynthesis and transpiration at the same WUE as the standard. The present work demonstrated that as pillar growth types are integrated into production systems, more research will be needed to efficiently schedule the water needs of the pillar architecture in order to maximize fruit size and quality due to the reduced LAD of this tree architecture and the greater illumination of the canopy interior. Published by Elsevier B.V. C1 [Glenn, D. M.; Bassett, C. B.; Tworkoski, T.; Scorza, R.; Miller, S. S.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov NR 20 TC 1 Z9 1 U1 2 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAY 13 PY 2015 VL 187 BP 30 EP 34 DI 10.1016/j.scienta.2015.02.030 PG 5 WC Horticulture SC Agriculture GA CH0ZT UT WOS:000353751600004 ER PT J AU Adjemian, MK Volpe, RJ Adjemian, J AF Adjemian, Michael K. Volpe, Richard J. Adjemian, Jennifer TI Relationships between Diet, Alcohol Preference, and Heart Disease and Type 2 Diabetes among Americans SO PLOS ONE LA English DT Article ID CARDIOVASCULAR RISK-FACTORS; LIFE-STYLE; INSULIN SENSITIVITY; HEMOSTATIC FACTORS; CROSSOVER TRIAL; SOCIAL-STATUS; US ADULTS; RED WINE; CONSUMPTION; MORTALITY AB Although excessive alcohol consumption is a recognized cause of morbidity and mortality, many studies have linked moderate alcohol consumption to improved cardiovascular health and a lower risk of Type 2 Diabetes (T2D). Self-reported alcohol and diet data used to generate these results suffer from measurement error due to recall bias. We estimate the effects of diet, alcohol, and lifestyle choices on the prevalence and incidence of cardiovascular disease and T2D among U.S. adults using a nationally representative cohort of households with scanner data representing their food-at-home, alcohol, and tobacco purchases from 2007-2010, and self-reported health surveys for the same study participants from 2010-2012. Multivariate regression models were used to identify significant associations among purchase data and lifestyle/demographic factors with disease prevalence in 2010, and with incidence of new disease from 2011-2012. After controlling for important confounders, respondents who purchased moderate levels of wine were 25% less likely than non-drinkers to report heart disease in 2010. However, no alcohol-related expenditure variables significantly affected the likelihood of reporting incident heart disease from 2011-2012. In contrast, many types of alcohol-related purchases were associated with a lower prevalence of T2D, and respondents who purchased the greatest volumes of wine or beer-but not liquor-were less likely to report being diagnosed with T2D in 2011-2012 than non-drinkers. C1 [Adjemian, Michael K.] USDA, Econ Res Serv, Washington, DC 20024 USA. [Volpe, Richard J.] Calif Polytech State Univ San Luis Obispo, Coll Agr Food & Environm Sci, Agribusiness Dept, San Luis Obispo, CA 93407 USA. [Adjemian, Jennifer] NIAID, Epidemiol Unit, Lab Clin Infect Dis, Bethesda, MD 20892 USA. [Adjemian, Jennifer] US Publ Hlth Serv Commissioned Corps, Rockville, MD USA. RP Adjemian, MK (reprint author), USDA, Econ Res Serv, Washington, DC 20024 USA. EM MAdjemian@ers.usda.gov FU National Institute of Allergy and Infectious Diseases, National Institutes of Health FX This research was supported in part by the intramural research program of the National Institute of Allergy and Infectious Diseases, National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 2 Z9 2 U1 1 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 11 PY 2015 VL 10 IS 5 AR e0124351 DI 10.1371/journal.pone.0124351 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI1YY UT WOS:000354542500011 PM 25961601 ER PT J AU Asada, M Yahata, K Hakimi, H Yokoyama, N Igarashi, I Kaneko, O Suarez, CE Kawazu, S AF Asada, Masahito Yahata, Kazuhide Hakimi, Hassan Yokoyama, Naoaki Igarashi, Ikuo Kaneko, Osamu Suarez, Carlos E. Kawazu, Shin-ichiro TI Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1 SO PLOS ONE LA English DT Article ID STABLE EXPRESSION; PLASMODIUM-BERGHEI; MALARIA PARASITES; GENE-EXPRESSION; SYSTEM; GENOME; MEROZOITES; DISRUPTION; TRANSIENT; SEQUENCE AB Genetic manipulation is an essential technique to analyze gene function; however, limited methods are available for Babesia bovis, a causative pathogen of the globally important cattle disease, bovine babesiosis. To date, two stable transfection systems have been developed for B. bovis, using selectable markers blasticidin-S deaminase (bsd) or human dihydrofolate reductase (hdhfr). In this work, we combine these two selectable markers in a sequential transfection system. Specifically, a parent transgenic B. bovis line which episomally expresses green fluorescent protein (GFP) and human dihydrofolate reductase (hDHFR), was transfected with a plasmid encoding a fusion protein consisting of red fluorescent protein (RFP) and blasticidin-S deaminase (BSD). Selection with WR99210 and blasticidin-S resulted in the emergence of parasites double positive for GFP and RFP. We then applied this method to complement gene function in a parasite line in which thioredoxin peroxidase-1 (Bbtpx-1) gene was knocked out using hDHFR as a selectable marker. A plasmid was constructed harboring both RFP-BSD and Bbtpx-1 expression cassettes, and transfected into a Bbtpx-1 knockout (KO) parasite. Transfectants were independently obtained by two transfection methods, episomal transfection and genome integration. Complementation of Bbtpx-1 resulted in full recovery of resistance to nitrosative stress, via the nitric oxide donor sodium nitroprusside, which was impaired in the Bbtpx-1 KO parasites. In conclusion, we developed a sequential transfection method in B. bovis and subsequently applied this technique in a gene complementation study. This method will enable broader genetic manipulation of Babesia toward enhancing our understanding of the biology of this parasite. C1 [Asada, Masahito; Yahata, Kazuhide; Hakimi, Hassan; Kaneko, Osamu] Nagasaki Univ, Inst Trop Med NEKKEN, Dept Protozool, Nagasaki 852, Japan. [Asada, Masahito; Hakimi, Hassan; Yokoyama, Naoaki; Igarashi, Ikuo; Kawazu, Shin-ichiro] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 080, Japan. [Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Suarez, Carlos E.] ARS, USDA, Anim Dis Res Unit, Pullman, WA USA. RP Suarez, CE (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. EM ces@vetmed.wsu.edu; skawazu@obihiro.ac.jp FU NRCPD-OUAVM Joint Research Grant of NRCPD, Obihiro University of Agriculture and Veterinary Medicine [24-11]; Cooperative Research Grant of NEKKEN, Nagasaki University [26-A-1]; JSPS KAKENHI, Japan society for the promotion of science [25850199] FX This work was supported by a NRCPD-OUAVM Joint Research Grant of NRCPD, Obihiro University of Agriculture and Veterinary Medicine (http://www.obihiro.ac.jp/similar to protozoa/eng/index-eng.html), to CES (No. 24-11), Cooperative Research Grant of NEKKEN, Nagasaki University (http://www.tm.nagasaki-u.ac.jp/nekken/english/index.html) to S-IK (No. 26-A-1) and JSPS KAKENHI, Japan society for the promotion of science (http://www.jsps.go.jp/english/index.html) to MA (No. 25850199). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 26 TC 2 Z9 2 U1 1 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 11 PY 2015 VL 10 IS 5 AR e0125993 DI 10.1371/journal.pone.0125993 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI1YY UT WOS:000354542500057 PM 25962142 ER PT J AU Hao, XY Chao, W Yang, YJ Horvath, D AF Hao, Xinyuan Chao, Wun Yang, Yajun Horvath, David TI Coordinated Expression of FLOWERING LOCUS T and DORMANCY ASSOCIATED MADS-BOX-Like Genes in Leafy Spurge SO PLOS ONE LA English DT Article ID PERSICA L. BATSCH; ARABIDOPSIS-THALIANA; CIRCADIAN CLOCK; BUD DORMANCY; CROWN BUDS; CARBOHYDRATE-METABOLISM; PROTEIN ACCUMULATION; VEGETATIVE GROWTH; CONSTANS; TRANSCRIPTION AB Leafy spurge (Euphorbia esula L.) is a noxious perennial weed that produces underground adventitious buds, which are crucial for generating new vegetative shoots following periods of freezing temperatures or exposure to various control measures. It is also capable of flowering and producing seeds, but requires vernalization in some cases. DORMANCY ASSOCIATED MADS-BOX (DAM) genes have been proposed to play a direct role in the transition to winter-induced dormancy and maintenance through regulation of the FLOWERING LOCUS T (FT) gene, which also is likely involved in the vernalization process. To explore the regulation of FT and DAM during dormancy transitions in leafy spurge, the transcript accumulation of two previously cloned DAM splice variants and two different previously cloned FT genes was characterized. Under long-photoperiods (16 h light), both DAM and FT transcripts accumulate in a diurnal manner. Tissue specific expression patterns indicated the tissues with high DAM expression had low FT expression and vice versa. DAM expression is detected in leaves, stems, shoot tips, and crown buds. FT transcripts were detected mainly in leaves and flowers. Under dormancy inducing conditions, DAM and FT genes had an inverse expression pattern. Additionally, chromatin immunoprecipitation assays were performed using DAM-like protein specific antibodies to demonstrate that DAM or related proteins likely bind to cryptic and/or conserved CArG boxes in the promoter regions of FT genes isolated from endodormant crown buds. These results are consistent with the hypothesis that DAM proteins play a crucial role in leafy spurge dormancy transition and maintenance, potentially by negatively regulating the expression of FT. C1 [Hao, Xinyuan; Yang, Yajun] Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Zhejiang, Peoples R China. [Hao, Xinyuan; Yang, Yajun] Minist Agr, Natl Ctr Tea Improvement, Key Lab Tea Biol & Resources Utilizat, Hangzhou 310008, Zhejiang, Peoples R China. [Hao, Xinyuan; Chao, Wun; Horvath, David] ARS, USDA, Biosci Res Lab, Fargo, ND 58105 USA. [Hao, Xinyuan] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. RP Horvath, D (reprint author), ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58105 USA. EM david.horvath@ars.usda.gov FU Novel Weed Management Solutions: a Basis in Understanding Bud and Seed Dormancy CRIS [3060-21220-026-00]; China Scholarship Council FX The work was supported by Novel Weed Management Solutions: a Basis in Understanding Bud and Seed Dormancy CRIS#3060-21220-026-00 and a graduate scholarship from the China Scholarship Council. NR 47 TC 4 Z9 4 U1 8 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 11 PY 2015 VL 10 IS 5 AR UNSP e0126030 DI 10.1371/journal.pone.0126030 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CI1YY UT WOS:000354542500059 PM 25961298 ER PT J AU Hussey, SG Mizrachi, E Groover, A Berger, DK Myburg, AA AF Hussey, Steven G. Mizrachi, Eshchar Groover, Andrew Berger, Dave K. Myburg, Alexander A. TI Genome-wide mapping of histone H3 lysine 4 trimethylation in Eucalyptus grandis developing xylem SO BMC PLANT BIOLOGY LA English DT Article DE ChIP-seq; H3K4me3; Histone; Secondary cell wall; Xylogenesis; Eucalyptus ID PREDICTIVE CHROMATIN SIGNATURES; CHIP-SEQ; GENE-EXPRESSION; METHYLATION PATTERNS; ARABIDOPSIS-THALIANA; DYNAMIC REGULATION; LIMITED NUMBERS; PLANTS; CELLS; ACTIVATION AB Background: Histone modifications play an integral role in plant development, but have been poorly studied in woody plants. Investigating chromatin organization in wood-forming tissue and its role in regulating gene expression allows us to understand the mechanisms underlying cellular differentiation during xylogenesis (wood formation) and identify novel functional regions in plant genomes. However, woody tissue poses unique challenges for using high-throughput chromatin immunoprecipitation (ChIP) techniques for studying genome-wide histone modifications in vivo. We investigated the role of the modified histone H3K4me3 (trimethylated lysine 4 of histone H3) in gene expression during the early stages of wood formation using ChIP-seq in Eucalyptus grandis, a woody biomass model. Results: Plant chromatin fixation and isolation protocols were optimized for developing xylem tissue collected from field-grown E. grandis trees. A "nano-ChIP-seq" procedure was employed for ChIP DNA amplification. Over 9 million H3K4me3 ChIP-seq and 18 million control paired-end reads were mapped to the E. grandis reference genome for peak-calling using Model-based Analysis of ChIP-Seq. The 12,177 significant H3K4me3 peaks identified covered similar to 1.5% of the genome and overlapped some 9,623 protein-coding genes and 38 noncoding RNAs. H3K4me3 library coverage, peaking similar to 600-700 bp downstream of the transcription start site, was highly correlated with gene expression levels measured with RNA-seq. Overall, H3K4me3-enriched genes tended to be less tissue-specific than unenriched genes and were overrepresented for general cellular metabolism and development gene ontology terms. Relative expression of H3K4me3-enriched genes in developing secondary xylem was higher than unenriched genes, however, and highly expressed secondary cell wall-related genes were enriched for H3K4me3 as validated using ChIP-qPCR. Conclusions: In this first genome-wide analysis of a modified histone in a woody tissue, we optimized a ChIP-seq procedure suitable for field-collected samples. In developing E. grandis xylem, H3K4me3 enrichment is an indicator of active transcription, consistent with its known role in sustaining pre-initiation complex formation in yeast. The H3K4me3 ChIP-seq data from this study paves the way to understanding the chromatin landscape and epigenomic architecture of xylogenesis in plants, and complements RNA-seq evidence of gene expression for the future improvement of the E. grandis genome annotation. C1 [Hussey, Steven G.; Mizrachi, Eshchar; Myburg, Alexander A.] Univ Pretoria, Forestry & Agr Biotechnol Inst, Genom Res Inst, Dept Genet, ZA-0028 Pretoria, South Africa. [Groover, Andrew] US Forest Serv, Pacific Southwest Res Stn, Davis, CA USA. [Groover, Andrew] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Berger, Dave K.] Univ Pretoria, Forestry & Agr Biotechnol Inst, Genom Res Inst, Dept Plant Sci, ZA-0028 Pretoria, South Africa. RP Myburg, AA (reprint author), Univ Pretoria, Forestry & Agr Biotechnol Inst, Genom Res Inst, Dept Genet, Private Bag X20, ZA-0028 Pretoria, South Africa. EM zander.myburg@fabi.up.ac.za RI Berger, Dave/B-5106-2008; Mizrachi, Eshchar/N-3179-2014; Myburg, Alexander/C-5426-2008; OI Berger, Dave/0000-0003-0634-1407; Myburg, Alexander/0000-0003-0644-5003; Hussey, Steven/0000-0002-1079-8373 FU Department of Science and Technology (DST), South Africa; National Research Foundation of South Africa (NRF) [UID 81111]; NRF Bioinformatics and Functional Genomics Program [UID 71255, UID 86936]; Mondi through the Forest Molecular Genetics (FMG) Program at the University of Pretoria (UP); Technology and Human Resources for Industry Program (THRIP) [UID 80118]; USDA National Institute of Food and Agriculture; Office of Science (BER), US Department of Energy; Sappi through the Forest Molecular Genetics (FMG) Program at the University of Pretoria (UP) FX E. grandis genomic sequence data were produced by the US Department of Energy Joint Genome Institute (http://www.jgi.doe.gov) in collaboration with the user community. SH, EM and AM acknowledge funding from the Department of Science and Technology (DST), South Africa, the National Research Foundation of South Africa (NRF) Incentive Funding for Rated Researchers Grant (UID 81111) and NRF Bioinformatics and Functional Genomics Program (UID 71255, UID 86936), Sappi and Mondi through the Forest Molecular Genetics (FMG) Program at the University of Pretoria (UP), and the Technology and Human Resources for Industry Program (THRIP) (UID 80118). Opinions expressed, and conclusions arrived at, are those of the author and are not necessarily to be attributed to the NRF. Mondi and Mmoledi Mphahlele (Mondi Tree Improvement Programme, Hilton, South Africa) are acknowledged for providing and collecting plant materials, respectively. Karen van der Merwe (UP) facilitated EucGenIE Gbrowse visualization of ChIP-seq data. Lijun Liu (UC Davis) is acknowledged for her mentorship in ChIP-seq. AG acknowledges funding from USDA National Institute of Food and Agriculture and the Office of Science (BER), US Department of Energy. NR 76 TC 2 Z9 3 U1 5 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 10 PY 2015 VL 15 AR 117 DI 10.1186/s12870-015-0499-0 PG 14 WC Plant Sciences SC Plant Sciences GA CH6TZ UT WOS:000354170500002 PM 25957781 ER PT J AU Schwalm, CR Huntzinger, DN Cook, RB Wei, Y Baker, IT Neilson, RP Poulter, B Caldwell, P Sun, G Tian, HQ Zeng, N AF Schwalm, C. R. Huntzinger, D. N. Cook, R. B. Wei, Y. Baker, I. T. Neilson, R. P. Poulter, B. Caldwell, Peter Sun, G. Tian, H. Q. Zeng, N. TI How well do terrestrial biosphere models simulate coarse-scale runoff in the contiguous United States? SO ECOLOGICAL MODELLING LA English DT Article DE Terrestrial biosphere models; Runoff; Intercomparison; North American Carbon Program; Regional ID AMERICAN REGIONAL REANALYSIS; GLOBAL VEGETATION MODEL; FRESH-WATER DISCHARGE; INTERANNUAL VARIABILITY; CIRCULATION MODEL; BALANCE; PRECIPITATION; CLIMATE; OCEAN; CYCLE AB Significant changes in the water cycle are expected under current global environmental change. Robust assessment of present-day water cycle dynamics at continental to global scales is confounded by shortcomings in the observed record. Modeled assessments also yield conflicting results which are linked to differences in model structure and simulation protocol. Here we compare simulated gridded (1 spatial resolution) runoff from six terrestrial biosphere models (TBMs), seven reanalysis products, and one gridded surface station product in the contiguous United States (CONUS) from 2001 to 2005. We evaluate the consistency of these 14 estimates with stream gauge data, both as depleted flow and corrected for net withdrawals (2005 only), at the CONUS and water resource region scale, as well as examining similarity across TBMs and reanalysis products at the grid cell scale. Mean runoff across all simulated products and regions varies widely (range: 71 to 356 mm yr(-1)) relative to observed continental-scale runoff (209 or 280 mm yr(-1) when corrected for net withdrawals). Across all 14 products 8 exhibit Nash-Sutcliffe efficiency values in excess of 0.8 and three are within 10% of the observed value. Region-level mismatch exhibits a weak pattern of overestimation in western and underestimation in eastern regions although two products are systematically biased across all regions and largely scales with water use. Although gridded composite TBM and reanalysis runoff show some regional similarities, individual product values are highly variable. At the coarse scales used here we find that progress in better constraining simulated runoff requires standardized forcing data and the explicit incorporation of human effects (e.g., water withdrawals by source, fire, and land use change). (C) 2015 Elsevier B.V. All rights reserved. C1 [Schwalm, C. R.] No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. [Schwalm, C. R.; Huntzinger, D. N.] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86011 USA. [Huntzinger, D. N.] No Arizona Univ, Dept Civil Engn Construct Management & Environm E, Flagstaff, AZ 86011 USA. [Cook, R. B.; Wei, Y.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Baker, I. T.] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. [Neilson, R. P.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Poulter, B.] Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. [Caldwell, Peter; Sun, G.] US Forest Serv, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Tian, H. Q.] Auburn Univ, Sch Forestry & Wildlife Sci, Int Ctr Climate & Global Change Res, Auburn, AL 36849 USA. [Zeng, N.] Univ Maryland, Dept Atmospher & Ocean Sci, College Pk, MD 20742 USA. RP Schwalm, CR (reprint author), No Arizona Univ, Ctr Ecosyst Sci & Soc, Flagstaff, AZ 86011 USA. EM christopher.schwalm@nau.edu RI Tian, Hanqin/A-6484-2012; OI Tian, Hanqin/0000-0002-1806-4091; Cook, Robert/0000-0001-7393-7302; Poulter, Benjamin/0000-0002-9493-8600 FU US National Aeronautics and Space Administration (NASA) Terrestrial Ecology Program [NNH10AN68I, NNX11AO08A]; Terrestrial Ecosystem Science Focus Area at the Office of Biological and Environmental Research, US Department of Energy (DOE); DOE [DE-AC05-00OR22725, DE-FG02-06ER64317]; National Science Foundation (NSF) [ATM-0425247, AGS-0425247-13, AGS1049041]; Department of Commerce/National Oceanic and Atmospheric Administration [NA08AR4320893]; NASA [NNX06AC75G, NNX08AM56G, NNX12AP86G, NNX10AT41G, NNX11AB87G]; National Institute for Climate Change Research [MTU050516Z14]; NSF EaSM program at North Carolina State University [AGS-1049200]; NASA Interdisciplinary Science Program [NNX11AD47G, NNX10AU06G] FX Support for this activity came from the US National Aeronautics and Space Administration (NASA) Terrestrial Ecology Program (NNH10AN68I and NNX11AO08A) and from the Terrestrial Ecosystem Science Focus Area at the Office of Biological and Environmental Research, US Department of Energy (DOE). Oak Ridge National Laboratory is managed by UT-Battelle for DOE under contract DE-AC05-00OR22725. IB was sponsored by the National Science Foundation (NSF) (ATM-0425247, AGS-0425247-13 and AGS1049041), the Department of Commerce/National Oceanic and Atmospheric Administration (NA08AR4320893), NASA (NNX06AC75G, NNX08AM56G, NNX12AP86G, NNX10AT41G, and NNX11AB87G), DOE (DE-FG02-06ER64317), and the National Institute for Climate Change Research (MTU050516Z14). PC and GS were partially supported by the NSF EaSM program (AGS-1049200) at North Carolina State University. HT was supported by the NASA Interdisciplinary Science Program (NNX11AD47G and NNX10AU06G). We thank Mac Post for his contributions to this research. This is a contribution of the North American Carbon Program. NR 59 TC 0 Z9 0 U1 3 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD MAY 10 PY 2015 VL 303 BP 87 EP 96 DI 10.1016/j.ecolmodel.2015.02.006 PG 10 WC Ecology SC Environmental Sciences & Ecology GA CG6PR UT WOS:000353424700009 ER PT J AU Amundson, R Berhe, AA Hopmans, JW Olson, C Sztein, AE Sparks, DL AF Amundson, Ronald Berhe, Asmeret Asefaw Hopmans, Jan W. Olson, Carolyn Sztein, A. Ester Sparks, Donald L. TI Soil and human security in the 21st century SO SCIENCE LA English DT Review ID ORGANIC-CARBON STOCKS; EARTH SYSTEM MODELS; CLIMATE-CHANGE; LAND-USE; UNITED-STATES; EROSION; BIODIVERSITY; FUTURE; CYCLE; SEDIMENTATION AB Human security has and will continue to rely on Earth's diverse soil resources. Yet we have now exploited the planet's most productive soils. Soil erosion greatly exceeds rates of production in many agricultural regions. Nitrogen produced by fossil fuel and geological reservoirs of other fertilizers are headed toward possible scarcity, increased cost, and/or geopolitical conflict. Climate change is accelerating the microbial release of greenhouse gases from soil organic matter and will likely play a large role in our near-term climate future. In this Review, we highlight challenges facing Earth's soil resources in the coming century. The direct and indirect response of soils to past and future human activities will play a major role in human prosperity and survival. C1 [Amundson, Ronald] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Berhe, Asmeret Asefaw] Univ Calif, Life & Environm Sci Unit, Merced, CA 95343 USA. [Hopmans, Jan W.] Land Air & Water Resources, Davis, CA 95616 USA. [Olson, Carolyn] USDA, Climate Change Program Off, Off Chief Economist, Washington, DC 20013 USA. [Sztein, A. Ester] Natl Acad Sci, Board Int Sci Org, Washington, DC 20001 USA. [Sparks, Donald L.] Univ Delaware, Plant & Soil Sci, Chem & Biochem, Civil & Environm Engn, Newark, DE 19716 USA. [Sparks, Donald L.] Univ Delaware, Marine Sci & Policy, Newark, DE 19716 USA. RP Amundson, R (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM earthy@berkeley.edu RI Amundson, Ronald /E-2654-2015 NR 61 TC 41 Z9 46 U1 58 U2 154 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD MAY 8 PY 2015 VL 348 IS 6235 AR 1261071 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH5AI UT WOS:000354045700035 PM 25954014 ER PT J AU Gaddis, KLP Tiezzi, F Cole, JB Clay, JS Maltecca, C AF Gaddis, Kristen L. Parker Tiezzi, Francesco Cole, John B. Clay, John S. Maltecca, Christian TI Genomic prediction of disease occurrence using producer-recorded health data: a comparison of methods SO GENETICS SELECTION EVOLUTION LA English DT Article ID ESTIMATED BREEDING VALUES; SOMATIC-CELL SCORE; CLINICAL MASTITIS; DAIRY-CATTLE; GENETIC-PARAMETERS; NORWEGIAN CATTLE; FULL PEDIGREE; HOLSTEIN COWS; SINGLE-STEP; VARIANCE-COMPONENTS AB Background: Genetic selection has been successful in achieving increased production in dairy cattle; however, corresponding declines in fitness traits have been documented. Selection for fitness traits is more difficult, since they have low heritabilities and are influenced by various non-genetic factors. The objective of this paper was to investigate the predictive ability of two-stage and single-step genomic selection methods applied to health data collected from on-farm computer systems in the U.S. Methods: Implementation of single-trait and two-trait sire models was investigated using BayesA and single-step methods for mastitis and somatic cell score. Variance components were estimated. The complete dataset was divided into training and validation sets to perform model comparison. Estimated sire breeding values were used to estimate the number of daughters expected to develop mastitis. Predictive ability of each model was assessed by the sum of chi(2) values that compared predicted and observed numbers of daughters with mastitis and the proportion of wrong predictions. Results: According to the model applied, estimated heritabilities of liability to mastitis ranged from 0.05 (SD = 0.02) to 0.11 (SD = 0.03) and estimated heritabilities of somatic cell score ranged from 0.08 (SD = 0.01) to 0.18 (SD = 0.03). Posterior mean of genetic correlation between mastitis and somatic cell score was equal to 0.63 (SD = 0.17). The single-step method had the best predictive ability. Conversely, the smallest number of wrong predictions was obtained with the univariate BayesA model. The best model fit was found for single-step and pedigree-based models. Bivariate single-step analysis had a better predictive ability than bivariate BayesA; however, the latter led to the smallest number of wrong predictions. Conclusions: Genomic data improved our ability to predict animal breeding values. Performance of genomic selection methods depends on a multitude of factors. Heritability of traits and reliability of genotyped individuals has a large impact on the performance of genomic evaluation methods. Given the current characteristics of producer-recorded health data, single-step methods have several advantages compared to two-step methods. C1 [Gaddis, Kristen L. Parker; Tiezzi, Francesco; Maltecca, Christian] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Cole, John B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Clay, John S.] Dairy Records Management Syst, Raleigh, NC 27603 USA. RP Gaddis, KLP (reprint author), N Carolina State Univ, Dept Anim Sci, Box 7621, Raleigh, NC 27695 USA. EM klpgaddis@ufl.edu RI Tiezzi, Francesco/L-6411-2016; Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Genus plc; Select Sires; [1265-31000-096-00] FX The authors would like to thank Dairy Records Management Systems, Raleigh, NC and USDA-ARS Animal Genomics and Improvement Laboratory, Beltsville, MD for providing the data, the Cooperative Dairy DNA Repository for providing the genotypes, and Ignacy Misztal's group at the University of Georgia (Athens) for providing software used for the genomic analysis. Partial funding for this research was provided by Genus plc and Select Sires. The contribution by scientists in the Animal Genomics and Improvement Laboratories was supported by appropriated project 1265-31000-096-00. NR 52 TC 2 Z9 2 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD MAY 8 PY 2015 VL 47 AR 41 DI 10.1186/s12711-015-0093-9 PG 13 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CH5NN UT WOS:000354082700001 ER PT J AU Renninger, HJ Carlo, NJ Clark, KL Schafer, KVR AF Renninger, Heidi J. Carlo, Nicholas J. Clark, Kenneth L. Schaefer, Karina V. R. TI Resource use and efficiency, and stomatal responses to environmental drivers of oak and pine species in an Atlantic Coastal Plain forest SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE photosynthesis; canopy conductance; sap flow; nitrogen-use efficiency; water-use efficiency ID WATER-USE EFFICIENCY; VAPOR-PRESSURE DEFICIT; CARBON-ISOTOPE DISCRIMINATION; NITROGEN-USE EFFICIENCY; DIFFUSE-POROUS TREES; QUERCUS-PUBESCENS; LOBLOLLY-PINE; SCOTS PINE; SAP FLOW; CANOPY CONDUCTANCE AB Pine-oak ecosystems are globally distributed even though differences in anatomy and leaf habit between many co-occurring oaks and pines suggest different strategies for resource use, efficiency and stomatal behavior. The New Jersey Pinelands contain sandy soils with low water- and nutrient-holding capacity providing an opportunity to examine trade-offs in resource uptake and efficiency. Therefore, we compared resource use in terms of transpiration rates and leaf nitrogen content and resource-use efficiency including water-use efficiency (WUE) via gas exchange and leaf carbon isotopes and photosynthetic nitrogen-use efficiency (PNUE) between oaks (Quercus alba, Q. prinus, Q. velutina) and pines (Pinus rigida, P echinata). We also determined environmental drivers [vapor pressure deficit (VPD), soil moisture, solar radiation] of canopy stomatal conductance (G(s)) estimated via sap flow and stomatal sensitivity to light and soil moisture. Net assimilation rates were similar between genera, but oak leaves used about 10% more water and pine foliage contained about 20% more N per unit leaf area. Therefore, oaks exhibited greater PNUE while pines had higher WUE based on gas exchange, although WUE from carbon isotopes was not significantly different. For the environmental drivers of Gs, oaks had about 10% lower stomatal sensitivity to VPD normalized by reference stomatal conductance compared with pines. Pines exhibited a significant positive relationship between shallow soil moisture and Gs, but only Gs in Q. velutina was positively related to soil moisture. In contrast, stomatal sensitivity to VPD was significantly related to solar radiation in all oak species but only pines at one site. Therefore, oaks rely more heavily on groundwater resources but have lower WUE, while pines have larger leaf areas and nitrogen acquisition but lower PNUE demonstrating a trade-off between using water and nitrogen efficiently in a resource-limited ecosystem. C1 [Renninger, Heidi J.; Schaefer, Karina V. R.] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. [Carlo, Nicholas J.; Schaefer, Karina V. R.] Rutgers State Univ, Dept Earth & Environm Sci, Newark, NJ 07102 USA. [Clark, Kenneth L.] USDA ARS, No Res Stn, Silas Little Expt Forest, New Lisbon, NJ USA. RP Renninger, HJ (reprint author), Mississippi State Univ, Dept Forestry, Thompson Hall,Box 9681, Mississippi State, MS 39762 USA. EM heidi.renninger@msstate.edu OI Renninger, Heidi/0000-0002-2485-9835 FU United States Department of Agriculture Forest Service [10-JV-11242306-136]; Office of Science (BER), United States Department of Energy [DE-5C0007041] FX The authors thank K. St. Ange, B. Tsai and M. Taeda for preparing leaf isotope samples. This research was funded by a United States Department of Agriculture Forest Service joint venture agreement 10-JV-11242306-136 and Office of Science (BER), United States Department of Energy DE-5C0007041 to KVRS. NR 72 TC 3 Z9 3 U1 9 U2 31 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 7 PY 2015 VL 6 AR 297 DI 10.3389/fpls.2015.00297 PG 16 WC Plant Sciences SC Plant Sciences GA CL4DB UT WOS:000356901700001 PM 25999966 ER PT J AU Collins, CDH Kautz, MA Tiller, R Lohani, S Ponce-Campos, G Hottenstein, J Metz, LJ AF Collins, Chandra D. Holifield Kautz, Mark A. Tiller, Ronald Lohani, Sapana Ponce-Campos, Guillermo Hottenstein, John Metz, Loretta J. TI Development of an integrated multiplatform approach for assessing brush management conservation efforts in semiarid rangelands SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE Landsat; aerial photography; vegetation change; woody cover; conservation; rangelands ID SPECTRAL MIXTURE ANALYSIS; NATIONAL RESOURCES INVENTORY; ADJUSTED VEGETATION INDEX; LAND-COVER; ACCURACY ASSESSMENT; SHRUB ENCROACHMENT; IMAGE-ANALYSIS; CANOPY COVER; NEW-MEXICO; GRASSLAND AB Millions of dollars have been spent on brush management, or removal of unwanted woody vegetation, as a conservation practice to control the presence of woody species. Land managers need an inexpensive means of monitoring the effects of brush management conservation methods for decreasing degradation in rangeland systems. In this study, free, publically available, high-resolution (1 m) imagery from the National Agricultural Imagery Program (NAIP) and moderate-resolution (30 m) Landsat-5 Thematic Mapper (TM) imagery were combined to produce a large-scale technique for mapping woody cover. High-resolution imagery-based estimates of woody cover were found to be reasonable (RMSE = 3.8%, MAE = 2.9%) surrogates for ground-based woody cover. An equation for TM-derived woody cover was developed. TM scenes of woody cover (TMWC) were produced and validated using NAIP and ground-based data. Results showed that the developed relation produced viable (RMSE = 8.5%, MAE = 6.4%) maps of woody cover that could be used to successfully track the occurrence of brush removal, as well as monitor the presence or lack of subsequent reemergence. This work provides land managers with an operational means of determining where to allocate resources to implement brush management, as well as a cost-effective method of monitoring the effects of their efforts. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. C1 [Collins, Chandra D. Holifield; Ponce-Campos, Guillermo] ARS, USDA, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Kautz, Mark A.; Lohani, Sapana; Hottenstein, John] Univ Arizona, Tucson, AZ 85721 USA. [Tiller, Ronald] Nature Conservancy, Tucson, AZ 85719 USA. [Metz, Loretta J.] USDA, NRCS Resource Assessment Div CEAP Grazing Lands, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Collins, CDH (reprint author), ARS, USDA, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM chandra.holifield@ars.usda.gov FU NRCS [67-3A75-14-115] FX This research was supported by NRCS Interagency Agreement 67-3A75-14-115. The authors wish to express sincere thanks to Mary White, Jason Wong, David Goodrich, and the personnel at the USDA-ARS Southwest Watershed Research Center, Emilio Carrillo from the USDA-NRCS area office in Tucson, Arizona, Lee Norfleet from the USDA-NRCS Resource Assessment Division (CEAP Modeling Team) in Temple, Texas, Ken Laterza and Tony Garcia from the USDA-NRCS Central Remote Sensing Laboratory in Ft. Worth, Texas, Veronica Lessard from the Iowa State University's Center for Survey Statistics and Methodology, and Stuart Marsh, Willem Van Leeuwen, Kyle Hartfield, and Andy Honaman from the University of Arizona, for their cooperation and assistance. Mention of proprietary product does not constitute a guarantee or warranty of the product by USDA or the author and does not imply its approval to the exclusion of other products that may also be suitable. NR 65 TC 1 Z9 1 U1 2 U2 8 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD MAY 7 PY 2015 VL 9 AR 096057 DI 10.1117/1.JRS.9.096057 PG 15 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CK5ON UT WOS:000356274900003 ER PT J AU O'Neill, KM Delphia, CM Pitts-Singer, TL AF O'Neill, Kevin M. Delphia, Casey M. Pitts-Singer, Theresa L. TI Seasonal trends in the condition of nesting females of a solitary bee: wing wear, lipid content, and oocyte size SO PEERJ LA English DT Article DE Megachile rotundata; Egg size; Reproduction; Megachilidae; Pollinators ID MEGACHILE-ROTUNDATA HYMENOPTERA; ALFALFA LEAFCUTTING BEES; INSECT FAT-BODY; SEX-RATIO; SEED FIELDS; OVARIAN DEVELOPMENT; UNITED-STATES; BUMBLE BEES; POLLINATOR; EMERGENCE AB During the nesting season, adult females of the solitary bee Megachile rotundata (F.) face considerable physical and energy demands that could include increasing wear and tear on their bodies and decreasing lipid reserves. Consequently, their reproductive performance may be affected not only by extrinsic factors (e.g., weather and floral resource availability), but intrinsic changes in their own bodies. Because of the potential fitness effects of seasonal changes in body condition, our objectives were to determine how wing wear, lipid reserves, and oocyte sizes vary during nesting seasons, beginning when females emerge as adults. As nesting progressed, females in two populations experienced a steady increase in wing wear, which is known to reduce foraging efficiency and increase risk of mortality in other bees. Soon after emergence, females exhibited sharp declines in lipid content which remained low for the remainder of the season. Newly-emerged females ingested pollen, an activity known to be correlated with the initiation of egg maturation in this species. Additionally, the early summer drop in lipid stores was correlated with an increase in the size of the oocytes carried. However, by similar to 6 weeks after emergence, oocytes began to decrease in length and volume, perhaps due to nutrient deficiencies related to loss of stored lipids. Our results suggest management of M. rotundata should include rearing bees at temperatures that maximize stored lipid reserves in adults and timing bee release so that significant pollen resources are available for both adults and offspring. C1 [O'Neill, Kevin M.; Delphia, Casey M.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Pitts-Singer, Theresa L.] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. RP O'Neill, KM (reprint author), Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. EM koneill@montana.edu FU Montana Agricultural Experiment Station; Montana Alfalfa Seed Growers Association; Montana Department of Agriculture; Western Alfalfa Seed Growers Association FX Funding for the research was supplied by the Montana Agricultural Experiment Station, the Montana Alfalfa Seed Growers Association, the Montana Department of Agriculture, the Western Alfalfa Seed Growers Association. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 62 TC 4 Z9 4 U1 4 U2 30 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD MAY 7 PY 2015 VL 3 AR e930 DI 10.7717/peerj.930 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH9XX UT WOS:000354391100001 PM 26019995 ER PT J AU Lozano, R Hamblin, MT Prochnik, S Jannink, JL AF Lozano, Roberto Hamblin, Martha T. Prochnik, Simon Jannink, Jean-Luc TI Identification and distribution of the NBS-LRR gene family in the Cassava genome SO BMC GENOMICS LA English DT Article ID LEUCINE-RICH REPEAT; NUCLEOTIDE-BINDING SITE; DISEASE RESISTANCE GENES; AXONOPODIS PV.-MANIHOTIS; BROWN STREAK DISEASE; ENCODING GENES; CELL-DEATH; MOSAIC GEMINIVIRUSES; BACTERIAL-BLIGHT; PROTEIN FAMILY AB Background: Plant resistance genes (R genes) exist in large families and usually contain both a nucleotide-binding site domain and a leucine-rich repeat domain, denoted NBS-LRR. The genome sequence of cassava (Manihot esculenta) is a valuable resource for analysing the genomic organization of resistance genes in this crop. Results: With searches for Pfam domains and manual curation of the cassava gene annotations, we identified 228 NBS-LRR type genes and 99 partial NBS genes. These represent almost 1% of the total predicted genes and show high sequence similarity to proteins from other plant species. Furthermore, 34 contained an N-terminal toll/interleukin (TIR)-like domain, and 128 contained an N-terminal coiled-coil (CC) domain. 63% of the 327 R genes occurred in 39 clusters on the chromosomes. These clusters are mostly homogeneous, containing NBS-LRRs derived from a recent common ancestor. Conclusions: This study provides insight into the evolution of NBS-LRR genes in the cassava genome; the phylogenetic and mapping information may aid efforts to further characterize the function of these predicted R genes. C1 [Lozano, Roberto; Jannink, Jean-Luc] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Hamblin, Martha T.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Prochnik, Simon] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. [Jannink, Jean-Luc] USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. EM jj332@cornell.edu FU project "Next Generation Cassava Breeding Project" through funds from the Bill and Melinda Gates foundation; Department for International Development of the United Kingdom FX This work was supported by the project "Next Generation Cassava Breeding Project" through funds from the Bill and Melinda Gates foundation and the Department for International Development of the United Kingdom. NR 82 TC 12 Z9 12 U1 4 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 7 PY 2015 VL 16 AR 360 DI 10.1186/s12864-015-1554-9 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CH3OC UT WOS:000353939000002 PM 25948536 ER PT J AU Pomeroy, J Bernhardt, M Marks, D AF Pomeroy, John Bernhardt, Matthias Marks, Daniel TI Research network to track alpine water SO NATURE LA English DT Letter C1 [Pomeroy, John] Univ Saskatchewan, Saskatoon, SK S7N 0W0, Canada. [Bernhardt, Matthias] BOKU Univ Nat Resources & Life Sci, Vienna, Austria. [Marks, Daniel] ARS, USDA, Boise, ID USA. RP Pomeroy, J (reprint author), Univ Saskatchewan, Saskatoon, SK S7N 0W0, Canada. EM matthias.bernhardt@boku.ac.at RI Bernhardt, Matthias/A-7168-2010 OI Bernhardt, Matthias/0000-0001-5503-7925 NR 0 TC 5 Z9 6 U1 2 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD MAY 7 PY 2015 VL 521 IS 7550 BP 32 EP 32 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH4YS UT WOS:000354040900019 PM 25951277 ER PT J AU Ding, F Duan, YP Paul, C Brlansky, RH Hartung, JS AF Ding, Fang Duan, Yongping Paul, Cristina Brlansky, Ronald H. Hartung, John S. TI Localization and Distribution of 'Candidatus Liberibacter asiaticus' in Citrus and Periwinkle by Direct Tissue Blot Immuno Assay with an Anti-OmpA Polyclonal Antibody SO PLOS ONE LA English DT Article ID REAL-TIME PCR; IN-SITU HYBRIDIZATION; MEDIATED ISOTHERMAL AMPLIFICATION; CA. L. AMERICANUS; GREENING DISEASE; HUANGLONGBING DISEASE; SWEET ORANGE; GENE-EXPRESSION; TRANSMISSION; INFECTION AB 'Candidatus Liberibacter asiaticus' (CaLas), a non-cultured member of the alpha-proteobacteria, is the causal agent of citrus Huanglongbing (HLB). Due to the difficulties of in vitro culture, antibodies against CaLas have not been widely used in studies of this pathogen. We have used an anti-OmpA polyclonal antibody based direct tissue blot immunoassay to localize CaLas in different citrus tissues and in periwinkle leaves. In citrus petioles, CaLas was unevenly distributed in the phloem sieve tubes, and tended to colonize in phloem sieve tubes on the underside of petioles in preference to the upper side of petioles. Both the leaf abscission zone and the junction of the petiole and leaf midrib had fewer CaLas bacteria compared to the main portions of the petiole and the midribs. Colonies of CaLas in phloem sieve tubes were more frequently found in stems with symptomatic leaves than in stems with asymptomatic leaves with an uneven distribution pattern. In serial sections taken from the receptacle to the peduncle, more CaLas were observed in the peduncle sections adjacent to the stem. In seed, CaLas was located in the seed coat. Many fewer CaLas were found in the roots, as compared to the seeds and petioles when samples were collected from trees with obvious foliar symptoms. The direct tissue blot immuno assay was adapted to whole periwinkle leaves infected by CaLas. The pathogen was distributed throughout the lateral veins and the results were correlated with results of qPCR. Our data provide direct spatial and anatomical information for CaLas in planta. This simple and scalable method may facilitate the future research on the interaction of CaLas and host plant. C1 [Ding, Fang; Paul, Cristina; Hartung, John S.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Ding, Fang] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China. [Duan, Yongping] USDA ARS, Hort Res Lab, Ft Pierce, FL USA. [Brlansky, Ronald H.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL USA. RP Hartung, JS (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM John.Hartung@ars.usda.gov FU Citrus Research and Development Foundation [551]; United States Department of Agriculture ARS [8042-22000-281-00D] FX Funding provided by Grant #551 Citrus Research and Development Foundation, http://citrusrdf.org/ to JSH and Project 8042-22000-281-00D, United States Department of Agriculture ARS to JSH. NR 58 TC 2 Z9 2 U1 3 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 6 PY 2015 VL 10 IS 5 AR e0123939 DI 10.1371/journal.pone.0123939 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH5BQ UT WOS:000354049700026 PM 25946013 ER PT J AU Mettke-Hofmann, C Hamel, PB Hofmann, G Zenzal, TJ Pellegrini, A Malpass, J Garfinkel, M Schiff, N Greenberg, R AF Mettke-Hofmann, Claudia Hamel, Paul B. Hofmann, Gerhard Zenzal, Theodore J., Jr. Pellegrini, Anne Malpass, Jennifer Garfinkel, Megan Schiff, Nathan Greenberg, Russell TI Competition and Habitat Quality Influence Age and Sex Distribution in Wintering Rusty Blackbirds SO PLOS ONE LA English DT Article ID MISSISSIPPI ALLUVIAL VALLEY; IDEAL FREE DISTRIBUTION; MIGRATORY BIRD; BODY CONDITION; BOTTOMLAND HARDWOODS; NONBREEDING SEASON; RIPARIAN FORESTS; DIFFERENT WIDTHS; FOOD RESOURCES; CLIMATE-CHANGE AB Bird habitat quality is often inferred from species abundance measures during the breeding and non-breeding season and used for conservation management decisions. However, during the non-breeding season age and sex classes often occupy different habitats which suggest a need for more habitat-specific data. Rusty Blackbird (Euphagus carolinus) is a forested wetland specialist wintering in bottomland hardwood forests in the south-eastern U.S. and belongs to the most steeply declining songbirds in the U.S. Little information is available to support priority birds such as the Rusty Blackbird wintering in this threatened habitat. We assessed age and sex distribution and body condition of Rusty Blackbirds among the three major habitats used by this species in the Lower Mississippi Alluvial Valley and also measured food availability. Overall, pecan groves had the highest biomass mainly driven by the amount of nuts. Invertebrate biomass was highest in forests but contributed only a small percentage to overall biomass. Age and sex classes were unevenly distributed among habitats with adult males primarily occupying pecan groves containing the highest nut biomass, females being found in forests which had the lowest nut biomass and young males primarily staying in forest fragments along creeks which had intermediate nut biomass. Males were in better body condition than females and were in slightly better condition in pecan groves. The results suggest that adult males occupy the highest quality habitat and may competitively exclude the other age and sex classes. C1 [Mettke-Hofmann, Claudia] Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 5UX, Merseyside, England. [Hamel, Paul B.; Schiff, Nathan] US Forest Serv, Stoneville, MS USA. [Hofmann, Gerhard] Tamar Grove, Moreton, Merseyside, England. [Zenzal, Theodore J., Jr.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. [Pellegrini, Anne] SWCA Environm Consultants, Flagstaff, AZ USA. [Malpass, Jennifer] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Garfinkel, Megan] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Mettke-Hofmann, Claudia; Greenberg, Russell] Smithsonian Inst, Natl Zool Pk, Smithsonian Migratory Bird Ctr, Washington, DC 20008 USA. RP Mettke-Hofmann, C (reprint author), Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 5UX, Merseyside, England. EM C.C.Mettke-Hofmann@ljmu.ac.uk FU U.S. Fish and Wildlife Service; Canadian Wildlife Service, Friends of the National Zoo, USA; Max Planck Institute for Ornithology, Andechs, Germany; German Ethological Society, Germany; Society for Tropical Ornithology, Germany; Arthur-von-Gwinner Foundation, Austria FX Assistants, travel and material were supported by the U.S. Fish and Wildlife Service (SMA grant, Quick Response Programme, Challenge Cost Share grant granted to the International Rusty Blackbird Technical Group). CM-H received support by the Canadian Wildlife Service, Friends of the National Zoo, USA, the Max Planck Institute for Ornithology, Andechs, Germany, the German Ethological Society, the Society for Tropical Ornithology, Germany, and the Arthur-von-Gwinner Foundation, Austria. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. SWCA Environmental Consultants provided support in the form of salaries for authors AP, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section. NR 64 TC 2 Z9 2 U1 4 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 6 PY 2015 VL 10 IS 5 AR e0123775 DI 10.1371/journal.pone.0123775 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH5BQ UT WOS:000354049700021 PM 25946335 ER PT J AU Bandara, T Hettiarachchi, M Liyanage, C Amarasena, S Wong, WWL AF Bandara, Thushari Hettiarachchi, Manjula Liyanage, Chandrani Amarasena, Sujeewa Wong, William Wai-Lun TI Body composition among Sri Lankan infants by O-18 dilution method and the validity of anthropometric equations to predict body fat against O-18 dilution SO BMC PEDIATRICS LA English DT Article DE Total body water; Fat mass; Fat free mass; Isotope ratio mass spectrometry; O-18 dilution; Skinfold thickness; Mid upper-arm circumference ID AIR-DISPLACEMENT PLETHYSMOGRAPHY; X-RAY ABSORPTIOMETRY; SKINFOLD THICKNESS; MIDARM CIRCUMFERENCE; YOUNG-CHILDREN; PERCENTAGE; DEUTERIUM; SAMPLES; WOMEN; WATER AB Background: Body composition indicators provide a better guidance for growth and nutritional status of the infants. This study was designed to (1) measure the body composition of the Sri Lankan infants using a reference method, the O-18 dilution method; (2) calculate the body fat content of the infants using published skinfold prediction equations; and (3) evaluate the applicability of the skinfold equations to predict body fat among Sri Lankan infants against the O-18 dilution method. Methods: Twenty five healthy, exclusively breast-fed infants were randomly recruited at well-baby clinics, for this cross-sectional study. Body composition was measured using O-18 dilution. Infant body weight, length, skinfold thicknesses and mid upper-arm circumference were measured using standard procedures. The Bland and Atlman pair-wise comparison method was used to evaluate the agreement of body fat generated using the anthropometric prediction equations against the O-18 dilution values as the reference. Results: Mean (SD) body weight and length of the infants were 6.5 kg (0.9) and 64.7 cm (2.8) respectively. Mean total body water, fat free mass, fat mass and % fat mass as measured by O-18 dilution method were 58.8% (5.0), 4.6 kg (0.8), 1.9 (0.5) and 29.5% (6.1). Total body water and fat free mass were significantly higher in boys when compared to girls. With the exception of three prediction equations (Bandana et al., Goran et al. and Durnin and Wormsley), most of the other commonly used anthropometry-based prediction equations yielded a bias which was not constant but a function of the % fat mass. Conclusions: Body composition of Sri Lankan infants is comparable to the normative data available from the industrialized countries. Most of the commonly used anthropometric prediction equations generated a bias which varies with the size of the body fat. Only three prediction equations (Bandana, Goran, Durnin & Wormsley) yield a constant bias. The Durnin & Wormsely equation showed the smallest bias when compared to the O-18 dilution values with the narrowest limits of agreement. Accuracy of some of the prediction equations is a function of gender. C1 [Bandara, Thushari; Hettiarachchi, Manjula; Liyanage, Chandrani; Amarasena, Sujeewa] Univ Ruhuna, Fac Med, Galle, Sri Lanka. [Wong, William Wai-Lun] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Bandara, T (reprint author), Univ Ruhuna, Fac Med, Galle, Sri Lanka. EM wvthush@yahoo.com FU International Atomic Energy Agency [IAEA-SRI-16826]; Lucinda L. Clarke at Baylor College of Medicine, USA FX This study was funded by the International Atomic Energy Agency, through research grant IAEA-SRI-16826. Funding body had no involvement with the study design, conducting the study or publication of the manuscript. We are extremely grateful to the medical officers and public health midwives of the selected MOH divisions and the committed mothers who participated in the study. The support by Lucinda L. Clarke at Baylor College of Medicine, USA for isotope analysis is greatly appreciated. The cooperation by the staff of the Nuclear Medicine Unit, Faculty of Medicine, Galle is also acknowledged. NR 32 TC 0 Z9 0 U1 2 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2431 J9 BMC PEDIATR JI BMC Pediatr. PD MAY 6 PY 2015 VL 15 AR 52 DI 10.1186/s12887-015-0371-2 PG 8 WC Pediatrics SC Pediatrics GA CH6QG UT WOS:000354160500001 PM 25943377 ER PT J AU Crawford, K Lager, K Miller, L Opriessnig, T Gerber, P Hesse, R AF Crawford, Kimberly Lager, Kelly Miller, Laura Opriessnig, Tanja Gerber, Priscilla Hesse, Richard TI Evaluation of porcine epidemic diarrhea virus transmission and the immune response in growing pigs SO VETERINARY RESEARCH LA English DT Article ID UNITED-STATES; OUTBREAK; SWINE; STRAINS; CHINA AB Clinical disease associated with porcine epidemic diarrhea virus (PEDV) infection in naive pigs is well chronicled; however, information on endemic PEDV infection is limited. To characterize chronic PEDV infection, the duration of infectious virus shedding and development of protective immunity was determined. On Day 0 (D0), a growing pig was challenged with PEDV and 13 contacts were commingled. On D7, 9 contact pigs (principal virus group (PG)), were selected, moved to a separate room and commingled with one sentinel pig (S1). This process was repeated weekly with S2, S3 and S4. The PG was PEDV-positive by PCR from D3-11, with some pigs intermittently positive to D42. Pigs S1 and S2 were PEDV-positive within 24 hours of commingling. Antibodies were detected in all PG by D21 and by 7 days post-contact in S1 and S2. Pigs S3 and S4 were PCR and antibody negative following commingling. To evaluate protective immunity, 5 naive pigs (N) and the PG were challenged (N/C, PG/C) with homologous virus on D49. All N/C pigs were PEDV PCR-positive by D52 with detection out to D62 in 3/5 N/C pigs. All PG/C pigs were PEDV PCR-negative post-challenge. By D63, all N/C seroconverted. Although PEDV RNA was demonstrated in pigs after primary infection until D42, infectious PEDV capable of horizontal transmission to naive pigs was only shed 14-16 days after infection to age-matched pigs. Homologous re-challenge 49 days post initial PEDV exposure did not result in re-infection of the pigs. This demonstrates potential for an effective PEDV vaccine. C1 [Crawford, Kimberly; Lager, Kelly; Miller, Laura] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. [Opriessnig, Tanja; Gerber, Priscilla] Iowa State Univ, Coll Vet Med, Vet Diagnost & Prod Anim Med, Ames, IA USA. [Opriessnig, Tanja; Gerber, Priscilla] Univ Edinburgh, Roslin Inst, Edinburgh EH8 9YL, Midlothian, Scotland. [Hesse, Richard] Kansas State Univ, Coll Vet Med, Diagnost Med Pathobiol, Manhattan, KS 66506 USA. RP Lager, K (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM Kelly.lager@ars.usda.gov RI Gerber, Priscilla/K-4771-2012; Opriessnig, Tanja/F-2984-2012 OI Gerber, Priscilla/0000-0002-8343-8299; Opriessnig, Tanja/0000-0001-9642-0904 NR 18 TC 10 Z9 10 U1 3 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD MAY 6 PY 2015 VL 46 AR 49 DI 10.1186/s13567-015-0180-5 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA CH2TM UT WOS:000353877600001 PM 25943434 ER PT J AU Akdemir, D Sanchez, JI Jannink, JL AF Akdemir, Deniz Sanchez, Julio I. Jannink, Jean-Luc TI Optimization of genomic selection training populations with a genetic algorithm SO GENETICS SELECTION EVOLUTION LA English DT Article ID RIDGE-REGRESSION; WIDE ASSOCIATION; PREDICTION; MAIZE; ACCURACY; TRAITS; MODELS; SET; L. AB In this article, we imagine a breeding scenario with a population of individuals that have been genotyped but not phenotyped. We derived a computationally efficient statistic that uses this genetic information to measure the reliability of genomic estimated breeding values (GEBV) for a given set of individuals (test set) based on a training set of individuals. We used this reliability measure with a genetic algorithm scheme to find an optimized training set from a larger set of candidate individuals. This subset was phenotyped to create the training set that was used in a genomic selection model to estimate GEBV in the test set. Our results show that, compared to a random sample of the same size, the use of a set of individuals selected by our method improved accuracies. We implemented the proposed training selection methodology on four sets of data on Arabidopsis, wheat, rice and maize. This dynamic model building process that takes genotypes of the individuals in the test sample into account while selecting the training individuals improves the performance of genomic selection models. C1 [Akdemir, Deniz; Sanchez, Julio I.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Jannink, Jean-Luc] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Akdemir, D (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. EM da346@cornell.edu OI Isidro-Sanchez, Julio/0000-0002-9044-3221 FU USDA-NIFA-AFRI Triticeae Coordinated Agricultural Project [2011-68002-30029] FX This research was supported by the USDA-NIFA-AFRI Triticeae Coordinated Agricultural Project, award number 2011-68002-30029. NR 35 TC 6 Z9 6 U1 4 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0999-193X EI 1297-9686 J9 GENET SEL EVOL JI Genet. Sel. Evol. PD MAY 6 PY 2015 VL 47 AR 38 DI 10.1186/s12711-015-0116-6 PG 10 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CH3PX UT WOS:000353943800001 PM 25943105 ER PT J AU Liang, JJ Zhou, M Tobin, PC McGuire, AD Reich, PB AF Liang, Jingjing Zhou, Mo Tobin, Patrick C. McGuire, A. David Reich, Peter B. TI Biodiversity influences plant productivity through niche-efficiency SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE biodiversity loss; marginal productivity; productivity impact index; relative productivity; biological conservation ID ECOSYSTEM PRODUCTIVITY; TEMPERATE FOREST; DIVERSITY; GROWTH AB The loss of biodiversity is threatening ecosystem productivity and services worldwide, spurring efforts to quantify its effects on the functioning of natural ecosystems. Previous research has focused on the positive role of biodiversity on resource acquisition (i.e., niche complementarity), but a lack of study on resource utilization efficiency, a link between resource and productivity, has rendered it difficult to quantify the biodiversity-ecosystem functioning relationship. Here we demonstrate that biodiversity loss reduces plant productivity, other things held constant, through theory, empirical evidence, and simulations under gradually relaxed assumptions. We developed a theoretical model named niche-efficiency to integrate niche complementarity and a heretofore-ignored mechanism of diminishing marginal productivity in quantifying the effects of biodiversity loss on plant productivity. Based on niche-efficiency, we created a relative productivity metric and a productivity impact index (PII) to assist in biological conservation and resource management. Relative productivity provides a standardized measure of the influence of biodiversity on individual productivity, and PII is a functionally based taxonomic index to assess individual species' inherent value in maintaining current ecosystem productivity. Empirical evidence from the Alaska boreal forest suggests that every 1% reduction in overall plant diversity could render an average of 0.23% decline in individual tree productivity. Out of the 283 plant species of the region, we found that large woody plants generally have greater PII values than other species. This theoretical model would facilitate the integration of biological conservation in the international campaign against several pressing global issues involving energy use, climate change, and poverty. C1 [Liang, Jingjing; Zhou, Mo] W Virginia Univ, Sch Nat Sci, Morgantown, WV 26506 USA. [Tobin, Patrick C.] USDA, Northern Res Stn, Forest Serv, Morgantown, WV 26505 USA. [McGuire, A. David] Univ Alaska, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Reich, Peter B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Reich, Peter B.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. RP Liang, JJ (reprint author), W Virginia Univ, Sch Nat Sci, Morgantown, WV 26506 USA. EM alpenbering@gmail.com OI Liang, Jingjing/0000-0001-9439-9320 FU Davis College of Agriculture, Natural Resources & Design, West Virginia University, under the US Department of Agriculture (USDA) McIntire-Stennis Funds [WVA00104, WVA00105]; School of Natural Resources and Extension, University of Alaska Fairbanks; Bonanza Creek Long-Term Ecological Research program - National Science Foundation (NSF); USDA Forest Service Pacific Northwest Research Station; Cedar Creek Long-Term Ecological Research program - NSF; Wilderness Research Foundation; Institute of the Environment, University of Minnesota FX We thank Professor Emeritus Joseph Buongiorno and Professors Jean-Paul Chavas, Jun Zhu, and James Moseley for their comments on the economic, statistical, and mathematical analysis. We also thank Professor and guest editor Charles Perrings, the anonymous reviewers, and Professor Christa P. H. Mulder for their helpful comments on the entire manuscript. Plant drawings in Fig. 1 are courtesy of Rebekah K. Watson. This study is supported in parts by the Davis College of Agriculture, Natural Resources & Design, West Virginia University, under the US Department of Agriculture (USDA) McIntire-Stennis Funds WVA00104 and WVA00105; School of Natural Resources and Extension, University of Alaska Fairbanks; the Bonanza Creek Long-Term Ecological Research program funded jointly by the National Science Foundation (NSF) and the USDA Forest Service Pacific Northwest Research Station; the Cedar Creek Long-Term Ecological Research program funded by NSF; and the Wilderness Research Foundation and Institute of the Environment, University of Minnesota. This is scientific article no. 3246 of the West Virginia Agricultural and Forestry Experiment Station, Morgantown. NR 31 TC 9 Z9 12 U1 24 U2 151 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 5 PY 2015 VL 112 IS 18 BP 5738 EP 5743 DI 10.1073/pnas.1409853112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH3TS UT WOS:000353953800060 PM 25901325 ER PT J AU Xiao, LH Yang, G Zhang, LC Yang, XH Zhao, S Ji, ZZ Zhou, Q Hu, M Wang, Y Chen, M Xu, Y Jin, HJ Xiao, X Hu, GP Bao, F Hu, Y Wan, P Li, LG Deng, X Kuang, TY Xiang, CB Zhu, JK Oliver, MJ He, YK AF Xiao, Lihong Yang, Ge Zhang, Liechi Yang, Xinhua Zhao, Shuang Ji, Zhongzhong Zhou, Qing Hu, Min Wang, Yu Chen, Ming Xu, Yu Jin, Haijing Xiao, Xuan Hu, Guipeng Bao, Fang Hu, Yong Wan, Ping Li, Legong Deng, Xin Kuang, Tingyun Xiang, Chengbin Zhu, Jian-Kang Oliver, Melvin J. He, Yikun TI The resurrection genome of Boea hygrometrica: A blueprint for survival of dehydration SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE vegetative desiccation tolerance; resurrection plant; Boea hygrometrica; drought tolerance enhancement; genome ID PLANT CRATEROSTIGMA-PLANTAGINEUM; VEGETATIVE DESICCATION TOLERANCE; MOLECULAR-MECHANISMS; GENES; EVOLUTION; SEQUENCE; ORGANIZATION; INSIGHTS; LIGHT AB "Drying without dying" is an essential trait in land plant evolution. Unraveling how a unique group of angiosperms, the Resurrection Plants, survive desiccation of their leaves and roots has been hampered by the lack of a foundational genome perspective. Here we report the similar to 1,691-Mb sequenced genome of Boea hygrometrica, an important resurrection plant model. The sequence revealed evidence for two historical genome-wide duplication events, a compliment of 49,374 protein-coding genes, 29.15% ofwhich are unique (orphan) to Boea and 20% of which (9,888) significantly respond to desiccation at the transcript level. Expansion of early light-inducible protein (ELIP) and 5S rRNA genes highlights the importance of the protection of the photosynthetic apparatus during drying and the rapid resumption of protein synthesis in the resurrection capability of Boea. Transcriptome analysis reveals extensive alternative splicing of transcripts and a focus on cellular protection strategies. The lack of desiccation tolerance-specific genome organizational features suggests the resurrection phenotype evolved mainly by an alteration in the control of dehydration response genes. C1 [Xiao, Lihong; Yang, Ge; Zhang, Liechi; Ji, Zhongzhong; Wang, Yu; Xu, Yu; Jin, Haijing; Xiao, Xuan; Hu, Guipeng; Bao, Fang; Hu, Yong; Wan, Ping; Li, Legong; He, Yikun] Capital Normal Univ, Sch Life Sci, Beijing 100048, Peoples R China. [Yang, Xinhua; Zhao, Shuang; Zhou, Qing; Hu, Min; Chen, Ming] Beijing Genom Inst Shenzhen, Shenzhen 518083, Peoples R China. [Deng, Xin] Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China. [Kuang, Tingyun] Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China. [Xiang, Chengbin] Univ Sci & Technol China, Sch Life Sci, Hefei 230022, Peoples R China. [Zhu, Jian-Kang] Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China. [Zhu, Jian-Kang] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Oliver, Melvin J.] Univ Missouri, USDA, Agr Res Serv, Midwest Area,Plant Genet Res Unit, Columbia, MO 65211 USA. RP Zhu, JK (reprint author), Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China. EM jkzhu@purdue.edu; Mel.Oliver@ars.usda.gov; yhe@cnu.edu.cn RI Zhu, Jian-Kang/F-7658-2011; OI Zhu, Jian-Kang/0000-0001-5134-731X; ji, Zhongzhong/0000-0003-0562-2477 FU Chinese Ministry of Agriculture [2014ZX08009-23B, 2009ZX08009-058B]; Chinese Ministry of Science and Technology [2007AA021405]; Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality; National Key Disciplines of China FX We thank the Beijing Genetics Institute staff members and Capital Normal University graduate students for their assistances on genome sequencing, assembling, and bioinformatic analyses. This study was supported by funds from the Chinese Ministry of Agriculture (2014ZX08009-23B, 2009ZX08009-058B), Chinese Ministry of Science and Technology (2007AA021405), and the Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality (Y. He). We are also thankful for the special financial support from the National Key Disciplines of China for this project. NR 35 TC 9 Z9 11 U1 5 U2 36 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 5 PY 2015 VL 112 IS 18 BP 5833 EP 5837 DI 10.1073/pnas.1505811112 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH3TS UT WOS:000353953800076 PM 25902549 ER PT J AU Kyndt, T Quispe, D Zhai, H Jarret, R Ghislain, M Liu, QC Gheysen, G Kreuze, JF AF Kyndt, Tina Quispe, Dora Zhai, Hong Jarret, Robert Ghislain, Marc Liu, Qingchang Gheysen, Godelieve Kreuze, Jan F. TI The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE horizontal gene transfer; Agrobacterium spp.; food safety; sweet potato; transgenic crops ID GENUS NICOTIANA; PHYLOGENETIC-RELATIONSHIPS; HORIZONTAL TRANSFER; GM CROPS; RHIZOGENES; EVOLUTION; PLASMID; SEQUENCE; IPOMOEA; TRANSFORMATION AB Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences from Agrobacterium spp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 and IbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas [L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant. IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes of Agrobacterium spp. IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives. IbT-DNA2 contained at least five ORFs with significant homology to the ORF14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n genes of A. rhizogenes. IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops. C1 [Kyndt, Tina; Quispe, Dora; Gheysen, Godelieve] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium. [Quispe, Dora; Ghislain, Marc; Kreuze, Jan F.] Int Potato Ctr, Lima 12, Peru. [Zhai, Hong; Liu, Qingchang] China Agr Univ, Beijing Key Lab Crop Genet Improvement, Lab Crop Heterosis & Utilizat, Minist Educ, Beijing 100193, Peoples R China. [Jarret, Robert] USDA ARS, Plant Genet Resources Unit, Griffin, GA 30223 USA. RP Kreuze, JF (reprint author), Int Potato Ctr, Lima 12, Peru. EM j.kreuze@cgiar.org RI Kyndt, Tina/E-4346-2013; OI Kyndt, Tina/0000-0002-5267-5013; Kreuze, Jan/0000-0002-6116-9200 FU Special Research Fund of Ghent University [BOF 01SB0710, 01W02112]; Consultative Group on International Agricultural Research (CGIAR) consortium research program on roots, tubers, and bananas FX We thank Lien De Smet, Isabelle Tilmant, Milton Untiveros, Bruno Lertora, Roland Robles, and Clauvis Taning for technical assistance; Martine De Cock and Ivonne Valdizan for help with layout and formatting; Wolfgang Gruneberg for sharing phenotypic and yield data for the BxT (Beauregard by Tanzania) population; Felipe de Mendiburo and Hannele Lindqvist Kreuze for advice on statistical analysis; Genoveva Rossel and David Tay for sharing ploidy information and DNA from germplasm accessions; Zengzhi Si for helping to screen the BAC library; and Jason McKinney (Fluidigm), Cameron Gundry, and Matt Poulson (BioFire Diagnostics) for high resolution melting primer design using sequence data provided by R. J. and screening of the sweet potato collection of the US Department of Agriculture. This work was supported by grants from the Special Research Fund of Ghent University (BOF 01SB0710 and 01W02112) and the Consultative Group on International Agricultural Research (CGIAR) consortium research program on roots, tubers, and bananas. T.K. is a postdoctoral fellow of the Research Foundation-Flanders. NR 56 TC 30 Z9 31 U1 14 U2 74 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 5 PY 2015 VL 112 IS 18 BP 5844 EP 5849 DI 10.1073/pnas.1419685112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH3TS UT WOS:000353953800078 PM 25902487 ER PT J AU Fanta, GF Selling, GW Felker, FC Kenar, JA AF Fanta, George F. Selling, Gordon W. Felker, Frederick C. Kenar, James A. TI Preparation and properties of films cast from mixtures of poly(vinyl alcohol) and submicron particles prepared from amylose-palmitic acid inclusion complexes SO CARBOHYDRATE POLYMERS LA English DT Article DE Starch; Amylose; Poly(vinyl alcohol); PVOH; Palmitic acid; Inclusion complex ID STARCH NANOCRYSTALS AB The use of starch in polymer composites for film production has been studied for increasing biodegradability, improving film properties and reducing cost. In this study, submicron particles were prepared from amylose-sodium palmitate complexes both by rapidly cooling jet-cooked starch-palmitic acid mixtures and by acidifying solutions of starch-sodium palmitate complexes. Films were cast containing poly(vinyl alcohol) (PVOH) with up to 50% starch particles. Tensile strength decreased and Young's modulus increased with starch concentration, but percent elongations remained similar to controls regardless of preparation method or starch content. Microscopy showed particulate starch distribution in films made with rapidly cooled starch-palmitic acid particles but smooth, diffuse starch staining with acidified sodium palmitate complexes. The mild effects on tensile properties suggest that submicron starch particles prepared from amylose-palmitic acid complexes provide a useful, commercially viable approach for PVOH film modification. Published by Elsevier Ltd. C1 [Fanta, George F.; Selling, Gordon W.] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Fanta, George F.; Selling, Gordon W.] ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Felker, Frederick C.; Kenar, James A.] ARS, Funct Foods Res Units, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Felker, FC (reprint author), ARS, Funct Foods Res Units, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM george.fanta@ars.usda.gov; gordon.selling@ars.usda.gov; jim.kenar@ars.usda.gov; frederick.felker@ars.usda.gov NR 17 TC 3 Z9 3 U1 4 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD MAY 5 PY 2015 VL 121 BP 420 EP 427 DI 10.1016/j.carbpol.2014.12.043 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA CC2NR UT WOS:000350182400053 PM 25659717 ER PT J AU Constanza, K Tallury, S Whaley, J Sanders, T Dean, L AF Constanza, Karen Tallury, Shyamalrau Whaley, Jeffrey Sanders, Timothy Dean, Lisa TI Chemical Composition of the Essential Oils from Leaves of Edible (Arachis hypogaea L.) and Perennial (Arachis glabrata Benth.) Peanut Plants SO JOURNAL OF ESSENTIAL OIL BEARING PLANTS LA English DT Article ID FRUITS C1 [Constanza, Karen] N Carolina State Univ, Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Tallury, Shyamalrau] Clemson Univ, Pee Dee Res Ctr, Crop Sci, Florence, SC 29506 USA. [Whaley, Jeffrey; Sanders, Timothy; Dean, Lisa] NCSU, USDA, ARS, Raleigh, NC 27695 USA. RP Dean, L (reprint author), NCSU, USDA, ARS, 236 D Schaub Hall, Raleigh, NC 27695 USA. EM Lisa.Dean@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 2 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0972-060X EI 0976-5026 J9 J ESSENT OIL BEAR PL JI J. Essent. Oil Bear. Plants. PD MAY 4 PY 2015 VL 18 IS 3 BP 605 EP 612 PG 8 WC Plant Sciences SC Plant Sciences GA CM4KZ UT WOS:000357654700011 ER PT J AU Hildebrandt, E Dunn, JR Cheng, HH AF Hildebrandt, Evin Dunn, John R. Cheng, Hans H. TI Characterizing in vivo stability and potential interactions of a UL5 helicase-primase mutation previously shown to reduce virulence and in vivo replication of Marek's disease virus SO VIRUS RESEARCH LA English DT Article DE Marek's disease virus (MDV); UL5 helicase-primase subunit; Attenuated; Replication; Virulence ID VACCINES; VACCINATION; ATTENUATION; EFFICACY; PASSAGE AB The unpredictable yet recurrent emergence of more virulent field strains of Marek's disease virus (MDV) in Marek's disease (MD) vaccinated flocks of chickens has prompted concerns regarding the sustainability of MD vaccines. A single non-synonymous point mutation (I682R) within the UL5 helicase-primase unit was shown to reduce virulence by over 90%. Considering in vitro attenuation is commonly used to generate MD vaccines, this result prompted further characterization of this mutation, particularly to better understand the potential of point mutations for use in vaccine development. Incorporation of a second non-synonymous point mutation (UL46-Q117R; tegument) found at high frequencies in the same attenuated MDV as the UL5 mutation did not further reduce virulence compared to the single UL5 mutation alone. Furthermore, when the UL5-containing MDV was serially passed three times in vivo, the resulting viruses did not show increases in replication or virulence, and no revertant viruses could be detected. This suggests that point mutations that reduce fitness and in vivo replication may be more stable than initially anticipated, which may alleviate some concerns regarding rationally designed MD vaccines based upon point mutations. Published by Elsevier B.V. C1 [Hildebrandt, Evin] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. [Hildebrandt, Evin; Dunn, John R.; Cheng, Hans H.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Cheng, HH (reprint author), USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM hans.cheng@ars.usda.gov NR 11 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD MAY 4 PY 2015 VL 203 BP 1 EP 3 DI 10.1016/j.virusres.2015.03.011 PG 3 WC Virology SC Virology GA CL2AO UT WOS:000356746200001 PM 25828576 ER PT J AU Ladinig, A Detmer, SE Clarke, K Ashley, C Rowland, RRR Lunney, JK Harding, JCS AF Ladinig, Andrea Detmer, Susan E. Clarke, Kyle Ashley, Carolyn Rowland, Raymond R. R. Lunney, Joan K. Harding, John C. S. TI Pathogenicity of three type 2 porcine reproductive and respiratory syndrome virus strains in experimentally inoculated pregnant gilts SO VIRUS RESEARCH LA English DT Article DE PRRSV; Pregnant gilt; Viral load; Pathogenicity; Cytokine; Fetal preservation ID EXPERIMENTALLY INFECTED SOWS; FETAL IMPLANTATION SITES; SYNDROME SIRS VIRUS; LELYSTAD VIRUS; UNITED-STATES; IN-UTERO; TRANSPLACENTAL INFECTION; MICROSCOPIC LESIONS; EUROPEAN GENOTYPE; SWINE INFERTILITY AB Mechanisms of reproductive failure resulting from infection with porcine reproductive and respiratory syndrome virus (PRRSV) are still poorly understood. Presented herein are the results of a side-by-side evaluation of the pathogenicity of three type 2 PRRSV strains in a reproductive model, from a pilot study used to develop experimental conditions and laboratory methods for a larger experiment. Pregnant gilts were experimentally infected with PRRSV at gestation day 85 or served as uninfected negative controls. After 21 days, all gilts and fetuses were necropsied. Clinical signs, litter outcome, viral load, cytokine levels, and pathology were compared from samples collected among pigs exposed to the three PRRSV strains. Based on differences in histologic lesions, and fetal weights, and numeric differences in gilt serum cytokine levels, litter outcome and virus replication in fetal tissues KS06-483 appeared less virulent than NVSL 97-7895 and KS06-72109 isolates. Levels of chemokine ligand 2 (CCL2), interferon alpha (IFN alpha), and interferon gamma (IFN gamma) were increased in PPRRSV-infected compared to non-infected gilts (0.01 > P < 0.06). Inoculation with NVSL 97-7895 induced higher levels of all three cytokines. All three PRRSV isolates were able to induce high mean viral load in individual litters, which was closely related to the proportion of PRRSV positive fetuses in the litter. Viral load in fetal samples was also positively associated with viral load at the maternal fetal interface. All but one dead fetus were positive for PRRSV RNA, and higher concentrations of PRRSV RNA in fetal thymus increased the odds of fetal death. Our results suggest that virus replication in fetal tissues and the maternal fetal interface, but not in other gilt tissues, are important for the outcome of reproductive PRRS. Additionally, our data indicate that umbilical lesions decreased corresponding to the use of pentobarbital sedation prior to euthanasia of pregnant gilts by captive bolt. (C) 2015 Elsevier B.V. All rights reserved. C1 [Ladinig, Andrea; Clarke, Kyle; Ashley, Carolyn; Harding, John C. S.] Univ Saskatchewan, Dept Large Anim Clin Sci, Western Coll Vet Med, Saskatoon, SK S7N 5B4, Canada. [Detmer, Susan E.] Univ Saskatchewan, Dept Vet Pathol, Western Coll Vet Med, Saskatoon, SK S7N 5B4, Canada. [Rowland, Raymond R. R.] Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA. [Lunney, Joan K.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Ladinig, A (reprint author), Univ Vet Med Vienna, Univ Clin Swine, Vet Pl 1, A-1210 Vienna, Austria. EM andrea.ladinig@vetmeduni.ac.at; susan.detmer@usask.ca; kyle.clarke@usask.ca; carolyn.ashley@usask.ca; browland@vet.k-state.edu; Joan.Lunney@ARS.USDA.GOV; john.harding@usask.ca OI Ladinig, Andrea/0000-0001-5037-7269 NR 52 TC 6 Z9 6 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD MAY 4 PY 2015 VL 203 BP 24 EP 35 DI 10.1016/j.virusres.2015.03.005 PG 12 WC Virology SC Virology GA CL2AO UT WOS:000356746200005 PM 25796212 ER PT J AU Mohideen, FW Stine, J Bechtel, PJ Solval, KM Bankston, JD Sathivel, S AF Mohideen, Fathima Waheeda Stine, Jesse Bechtel, Peter J. Solval, Kevin Mis Bankston, J. David Sathivel, Subramaniam TI Effects of Blueberry (Vaccinium corymbosum) Juice on Lipid Oxidation During Spray Drying of Microencapsulated Menhaden Oil SO INTERNATIONAL JOURNAL OF FOOD PROPERTIES LA English DT Article DE Microencapsulation; Lipid oxidation; Menhaden oil; Blueberry juice; Spray drying ID POLYUNSATURATED FATTY-ACIDS; FISH-OIL; ANTIOXIDANT CAPACITY; PARTICLE-SIZE; STABILITY; ENCAPSULATION; PRODUCTS; IMPACT; STORAGE AB This study investigated the effect of blueberry juice on menhaden oil lipid oxidation during microencapsulation. Oil in water emulsions containing menhaden oil with 0, 5, or 10% blueberry juice were spray dried to produce control-M, 5% BJ-M, and 10% BJ-M microencapsulated powders, respectively. All microencapsulated powders had similar encapsulation efficiencies with low surface oil content. Peroxide value (meq/kg of oil) was 4.50, 4.31, and 3.38 for control-M, 5% BJ-M and 10% BJ-M, respectively. Ten percent BJ-M had lower (P < 0.05) anisidine value, and totox values than 5% BJ-M and control-M. This indicated that 10% blueberry juice reduced lipid oxidation in menhaden oil during microencapsulation to a greater extent than the other formulations. C1 [Mohideen, Fathima Waheeda; Bankston, J. David; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Dept Food Sci, Baton Rouge, LA 70803 USA. [Stine, Jesse] Salish Kootenai Coll, Pablo, MT USA. [Bechtel, Peter J.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. [Solval, Kevin Mis; Sathivel, Subramaniam] Louisiana State Univ, Ctr Agr, Dept Biol & Agr Engn, Baton Rouge, LA 70803 USA. RP Sathivel, S (reprint author), Louisiana State Univ, Ctr Agr, Dept Food Sci, 111 Food Sci Bldg, Baton Rouge, LA 70803 USA. EM ssathivel@agcenter.lsu.edu NR 36 TC 1 Z9 1 U1 3 U2 15 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1094-2912 EI 1532-2386 J9 INT J FOOD PROP JI Int. J. Food Prop. PD MAY 4 PY 2015 VL 18 IS 5 BP 1139 EP 1153 DI 10.1080/10942912.2013.877025 PG 15 WC Food Science & Technology SC Food Science & Technology GA CJ1LF UT WOS:000355244400019 ER PT J AU Krysanova, V White, M AF Krysanova, Valentina White, Mike TI Advances in water resources assessment with SWAT-an overview SO HYDROLOGICAL SCIENCES JOURNAL-JOURNAL DES SCIENCES HYDROLOGIQUES LA English DT Article DE climate change impact; land-use change impact; SWAT; nonpoint-source pollution; water resources assessment ID ASSESSMENT-TOOL SWAT; CLIMATE-CHANGE; QUALITY MODEL; LAND-USE; AGRICULTURAL WATERSHEDS; MANAGEMENT-PRACTICES; RIVER-BASINS; SOIL; STREAMFLOW; IMPACTS AB This paper introduces a Special Issue of Hydrological Sciences Journal containing 10 research papers which present current applications of the Soil and Water Assessment Tool (SWAT) for water resources assessment. First, an overview of selected, recently published papers with application of SWAT is given. The papers address the following topics: nutrients and related best management practices (BMPs); sediments and related BMPs; impoundment and wetlands; irrigation; bioenergy crops; climate change impact; and land-use change impacts. Then, papers from this Special Issue are briefly described, covering the themes: surface runoff and sediments; nonpoint-source pollution; surface water and groundwater; impacts of climate and land-use change; and large-scale SWAT applications. The presented model applications of SWAT were conducted across a variety of spatial scales, physiographic regions and climatic zones. This collection of papers demonstrates that applications of SWAT for water resources assessment are growing in number and cover drainage basins in many regions worldwide. [GRAPHICS] Editor M. Acreman; Associate editor Xi Chen C1 [Krysanova, Valentina] Potsdam Inst Climate Impact Res, Potsdam, Germany. [White, Mike] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Krysanova, V (reprint author), Potsdam Inst Climate Impact Res, Potsdam, Germany. EM krysanova@pik-potsdam.de NR 84 TC 12 Z9 12 U1 10 U2 55 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0262-6667 EI 2150-3435 J9 HYDROLOG SCI J JI Hydrol. Sci. J.-J. Sci. Hydrol. PD MAY 4 PY 2015 VL 60 IS 5 SI SI BP 771 EP 783 DI 10.1080/02626667.2015.1029482 PG 13 WC Water Resources SC Water Resources GA CI3CB UT WOS:000354624000001 ER PT J AU Chang, PK Scharfenstein, LL Solorzano, CD Abbas, HK Hua, SST Jones, WA Zablotowicz, RM AF Chang, Perng-Kuang Scharfenstein, Leslie L. Solorzano, Cesar D. Abbas, Hamed K. Hua, Sui-Sheng T. Jones, Walker A. Zablotowicz, Robert M. TI High sequence variations in the region containing genes encoding a cellular morphogenesis protein and the repressor of sexual development help to reveal origins of Aspergillus oryzae SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Aspergillus oryzae; Aspergillus flavus; Deletion; Genome; Recombination ID CYCLOPIAZONIC ACID; MOLECULAR CHARACTERIZATION; POPULATION-STRUCTURE; AFLATOXIN PRODUCTION; FLANKING REGIONS; SECTION FLAVI; STRAINS; CLUSTER; BIOSYNTHESIS; GENOME AB Aspergillus oryzae and Aspergillus flavus are closely related fungal species. The A. flavus morphotype that produces numerous small sclerotia (S strain) and aflatoxin has a unique 1.5 kb deletion in the norB-cypA region of the aflatoxin gene cluster (i.e. the S genotype). Phylogenetic studies have indicated that an isolate of the nonaflatoxigenic A. flavus with the S genotype is the ancestor of A. oryzae. Genome sequence comparison between A. flavits NRRL3357, which produces large sclerotia (L strain), and S-strain A. flavus 70S identified a region (samA-rosA) that was highly variable in the two morphotypes. A third type of samA-rosA region was found in A. oryzae RIB40. The three samA-rosA types were later revealed to be commonly present in A. flavus L-strain populations. Of the 182 L-strain A. flavus field isolates examined, 46%, 15% and 39% had the samA-rosA type of NRRL3357, 70S and RIB40, respectively. The three types also were found in 18 S-strain A. flavus isolates with different proportions. For A. oryzae, however, the majority (80%) of the 16 strains examined had the RIB40 type and none had the NRRL3357 type. The results suggested that A. oryzae strains in the current culture collections were mostly derived from the samA-rosA/RIB40 lineage of the nonaflatoxigenic A. flavus with the S genotype. Published by Elsevier B.V. C1 [Chang, Perng-Kuang; Scharfenstein, Leslie L.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Solorzano, Cesar D.; Abbas, Hamed K.; Jones, Walker A.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Hua, Sui-Sheng T.] USDA, ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Zablotowicz, Robert M.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Chang, PK (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM perngkuang.chang@ars.usda.gov NR 49 TC 1 Z9 1 U1 5 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD MAY 4 PY 2015 VL 200 BP 66 EP 71 DI 10.1016/j.ijfoodmicro.2015.01.021 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CE7VO UT WOS:000352050200011 PM 25689355 ER PT J AU Kingsley, DC Brown, TC AF Kingsley, David C. Brown, Thomas C. TI Endogenous and costly institutional deterrence SO APPLIED ECONOMICS LETTERS LA English DT Article DE public goods; endogenous deterrence; formal institutions; central authority; C92; H41; D70 ID PUBLIC-GOODS; GAMES AB Modern economies rely on central-authority institutions to regulate individual behaviour. Despite the importance of such institutions little is known about their formation within groups. In a public good experiment, groups selected the level of deterrence implemented by the institution, knowing that the administrative costs of the institution rose with the level of deterrence. Results suggest that groups readily self-impose costly deterrent formal institutions. The strictly deterrent institutions implemented here increased contributions sufficiently to completely offset the administrative cost and significantly increase earnings. C1 [Kingsley, David C.] Univ Massachusetts Lowell, Dept Econ, Lowell, MA 01854 USA. [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Kingsley, DC (reprint author), Univ Massachusetts Lowell, Dept Econ, Lowell, MA 01854 USA. EM david_kingsley@uml.edu NR 8 TC 0 Z9 0 U1 2 U2 3 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 1350-4851 EI 1466-4291 J9 APPL ECON LETT JI Appl. Econ. Lett. PD MAY 3 PY 2015 VL 22 IS 7 BP 544 EP 548 DI 10.1080/13504851.2014.955252 PG 5 WC Economics SC Business & Economics GA CB7IO UT WOS:000349800400007 ER PT J AU Xie, T Reddy, KR Wang, CW Yargicoglu, E Spokas, K AF Xie, Tao Reddy, Krishna R. Wang, Chengwen Yargicoglu, Erin Spokas, Kurt TI Characteristics and Applications of Biochar for Environmental Remediation: A Review SO CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY LA English DT Review DE remediation; biomass; biochar; sorption; pyrolysis ID POLYCYCLIC AROMATIC-HYDROCARBONS; SLUDGE-DERIVED BIOCHAR; WHEAT-STRAW BIOCHAR; SUGAR-BEET TAILINGS; PYROLYSIS TEMPERATURE; HYDROTHERMAL CARBONIZATION; ACTIVATED CARBON; SURFACE-CHEMISTRY; BLACK CARBON; RICE-STRAW AB Biochar is a stabilized, recalcitrant organic carbon compound, created when biomass is heated to temperatures usually between 300 and 1000 degrees C, under low (preferably zero) oxygen concentrations. It is produced from a variety of biomass feedstock, such as agricultural residues, wood chips, manure, and municipal solid waste, through a variety of thermal treatments, among which slow pyrolysis is the most widely used due to its moderate operating conditions and optimization of biochar yields. Despite the recent introduction of the term biochar for this material, there have been several applications of charred materials in the past due to their unique properties (e.g., high specific surface area, microporosity, and sorptive capabilities). These early applications have primarily focused on the use of biochar as a soil amendment in agriculture, though other applications in environmental remediation engineering may be equally important (i.e., for soil and groundwater treatment and stormwater filter media). The objective of this review is to provide a detailed examination into the engineering properties and potential uses of biochar as an engineered material for environmental remediation. Biochar, due its highly variable and customizable surface chemistry, offers great potential in a variety of engineering applications, some of which have yet to be discovered. C1 [Xie, Tao; Wang, Chengwen] Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R China. [Reddy, Krishna R.; Yargicoglu, Erin] Univ Illinois, Dept Civil & Mat Engn, Chicago, IL 60607 USA. [Spokas, Kurt] USDA ARS, Minneapolis, MN USA. [Spokas, Kurt] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Reddy, KR (reprint author), Univ Illinois, Dept Civil & Mat Engn, 842 West Taylor St, Chicago, IL 60607 USA. EM kreddy@uic.edu RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 FU U.S. National Science Foundation [1200799]; China Scholarship Council FX Financial support for this project is provided by the U.S. National Science Foundation (Grant CMMI #1200799) and the China Scholarship Council, which is gratefully acknowledged. NR 134 TC 28 Z9 31 U1 84 U2 797 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1064-3389 EI 1547-6537 J9 CRIT REV ENV SCI TEC JI Crit. Rev. Environ. Sci. Technol. PD MAY 3 PY 2015 VL 45 IS 9 BP 939 EP 969 DI 10.1080/10643389.2014.924180 PG 31 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY6SC UT WOS:000347694800002 ER PT J AU Mai, K Elder, T Groom, LH Spivey, JJ AF Mai, Khiet Elder, Thomas Groom, Leslie H. Spivey, James J. TI Fe-based Fischer Tropsch synthesis of biomass-derived syngas: Effect of synthesis method SO CATALYSIS COMMUNICATIONS LA English DT Article DE Biomass syngas; Fischer Tropsch; Fe-based; Coprecipitation; Impregnation ID CATALYSTS AB Two 100Fe/4Cu/4K/6Zn catalysts were prepared using two different methods: coprecipitation or impregnation methods. The effect of the preparation methods on the catalyst structure, catalytic properties, and the conversion of biomass-derived syngas via Fischer-Tropsch synthesis was investigated. Syngas was derived from gasifying Southern pine woodchips and had the composition of 3.1% CH4, 11% CO2, 17% H-2, 22% CO, and 47% N-2. The results show that the coprecipitated Zn-promoted catalyst had higher extent of reduction and carburization, which led to higher carbon conversion and C5+ selectivity of this CO2-rich syngas than the impregnated catalyst. (C) 2015 Elsevier B.V. All rights reserved. C1 [Mai, Khiet; Spivey, James J.] Louisiana State Univ, Dept Chem Engn, Baton Rouge, LA 70803 USA. [Elder, Thomas; Groom, Leslie H.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. RP Spivey, JJ (reprint author), Louisiana State Univ, Dept Chem Engn, S Stadium Dr, Baton Rouge, LA 70803 USA. EM jjspivey@lsu.edu FU U.S. Department of Agriculture [11-DG-11221636-187] FX The authors gratefully acknowledge the financial support from the U.S. Department of Agriculture, under Award Number 11-DG-11221636-187. NR 12 TC 3 Z9 4 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1566-7367 EI 1873-3905 J9 CATAL COMMUN JI Catal. Commun. PD MAY 2 PY 2015 VL 65 BP 76 EP 80 DI 10.1016/j.catcom.2015.02.027 PG 5 WC Chemistry, Physical SC Chemistry GA CG0ZR UT WOS:000353003300016 ER PT J AU Fowler, JF Sieg, CH Hedwall, S AF Fowler, James F. Sieg, Carolyn Hull Hedwall, Shaula TI BASELINES TO DETECT POPULATION STABILITY OF THE THREATENED ALPINE PLANT PACKERA FRANCISCANA (ASTERACEAE) SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID CLIMATE-CHANGE; RANGE SHIFTS; VEGETATION; DIVERSITY; DENSITY; EUROPE AB Population size and density estimates have traditionally been acceptable ways to track species' response to changing environments; however, species' population centroid elevation has recently been an equally important metric. Packera franciscana (Greene) W.A. Weber and A. Love (Asteraceae; San Francisco Peaks ragwort) is a single mountain endemic plant found only in upper treeline and alpine talus habitats of the San Francisco Peaks in northern Arizona and is listed by the U.S. Fish and Wildlife Service as a Threatened species under the Endangered Species Act. We used 2 recreational trails, which pass through its elevational distribution in the Kachina Peaks Wilderness Area, as sampling transects to estimate population density and distribution because it was not possible to sample further from the trail due to loose volcanic talus and consequent disturbance of P. franciscana plants. Between-year comparisons of ramet density, population centroid elevations, and mid-September flowering or fruiting counts were not significantly different for the Humphrey's Peak Trail in 2010-2011 or the Weatherford Trail from 2009 to 2013. Use of a zero-inflated negative binomial response variable distribution allowed statistical inference for both sampling hits and misses, which gives ecologists and land managers an additional method to monitor changes in distribution and abundance. Population density and elevation of the population centroid estimates for P. franciscana appear to be stable; therefore, the 5-year Weatherford Trail data set may be used as a baseline to track future plant migration on a fine spatiotemporal scale, an approach that could apply to other threatened alpine species. C1 [Fowler, James F.; Sieg, Carolyn Hull] US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Hedwall, Shaula] US Fish & Wildlife Serv, Flagstaff, AZ 86001 USA. RP Fowler, JF (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM alpineworkshop@gmail.com FU Coconino National Forest; Rocky Mountain Research Station, Forest and Woodland Ecoystems Program FX Funding for this research was provided by the Coconino National Forest and the Rocky Mountain Research Station, Forest and Woodland Ecoystems Program. We thank our field crew members for dedication to this work: Steve Overby, Brian Casavant, Addie Hite, Scott Vojta, and Suzanne Owen. Voucher specimens were collected under U.S. Fish and Wildlife permit TE225821-0 and are deposited in the RMRS Flagstaff Herbarium (ASUF). NR 32 TC 1 Z9 1 U1 2 U2 3 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD MAY PY 2015 VL 75 IS 1 BP 70 EP 77 DI 10.3398/064.075.0108 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NO UT WOS:000370893000007 ER PT J AU Backus, EA Shugart, HJ Rogers, EE Morgan, JK Shatters, R AF Backus, Elaine A. Shugart, Holly J. Rogers, Elizabeth E. Morgan, J. Kent Shatters, Robert TI Direct Evidence of Egestion and Salivation of Xylella fastidiosa Suggests Sharpshooters Can Be "Flying Syringes" SO PHYTOPATHOLOGY LA English DT Article DE acquisition; Pierce's disease ID GLASSY-WINGED SHARPSHOOTER; PIERCES DISEASE BACTERIUM; FASCIFRONS STAL HOMOPTERA; POTATO VIRUS-Y; PLANT-VIRUSES; VECTOR TRANSMISSION; STYLET PENETRATION; INSECT VECTOR; IN-VITRO; APHIDS AB Xylella fastidiosa is unique among insect-transmitted plant pathogens because it is propagative but noncirculative, adhering to and multiplying on the cuticular lining of the anterior foregut. Any inoculation mechanism for X. fastidiosa must explain how bacterial cells exit the vector's stylets via the food canal and directly enter the plant. A combined egestion-salivation mechanism has been proposed to explain these unique features. Egestion is the putative outward flow of fluid from the foregut via hypothesized bidirectional pumping of the cibarium. The present study traced green fluorescent protein-expressing X. fastidiosa or fluorescent nanoparticles acquired from artificial diets by glassy-winged sharpshooters, Homalodisca vitripennis, as they were egested into simultaneously secreted saliva. X. fastidiosa or nanoparticles were shown to mix with gelling saliva to form fluorescent deposits and salivary sheaths on artificial diets, providing the first direct, conclusive evidence of egestion by any hemipteran insect. Therefore, the present results strongly support an egestion-salivation mechanism of X. fastidiosa inoculation. Results also support that a column of fluid is transiently held in the foregut without being swallowed. Evidence also supports (but does not definitively prove) that bacteria were suspended in the column of fluid during the vector's transit from diet to diet, and were egested with the held fluid. Thus, we hypothesize that sharpshooters could be true "flying syringes," especially when inoculation occurs very soon after uptake of bacteria, suggesting the new paradigm of a nonpersistent X. fastidiosa transmission mechanism. C1 [Backus, Elaine A.; Rogers, Elizabeth E.] USDA ARS, San Joaquin Valley Agr Sci Ctr, US Dept Agr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. [Shugart, Holly J.] Univ Florida, Dept Entomol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Morgan, J. Kent; Shatters, Robert] USDA ARS, US Hort Res Lab, Subtrop Insects & Hort Res, Ft Pierce, FL 34945 USA. RP Backus, EA (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, US Dept Agr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Elaine.Backus@ars.usda.gov FU ARS FX H. vitripennis were generously provided from a colony maintained by W. Hunter at the U.S. Department of Agriculture (USDA)-Agricultural Research Service (ARS), U.S. Horticultural Research Laboratory at Fort Pierce, FL. We also thank S. Lindow, University of California, Berkeley, for providing the original culture of GFP-transformed X. fastidiosa used in this study. Sharpshooters and plants were reared by C. Cooper and D. Schletewitz, and PCR tests were performed by M. Schreiber (all at ARS, Parlier), whose help is also greatly appreciated. Funding for this project came from in-house ARS support to E. A. Backus and E. E. Rogers. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 56 TC 3 Z9 3 U1 0 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2015 VL 105 IS 5 BP 608 EP 620 DI 10.1094/PHYTO-09-14-0258-R PG 13 WC Plant Sciences SC Plant Sciences GA CJ2JW UT WOS:000355311500004 PM 26020829 ER PT J AU Short, DPG Sandoya, G Vallad, GE Koike, ST Xiao, CL Wu, BM Gurung, S Hayes, RJ Subbarao, KV AF Short, Dylan P. G. Sandoya, German Vallad, Gary E. Koike, Steven T. Xiao, Chang-Lin Wu, Bo-Ming Gurung, Suraj Hayes, Ryan J. Subbarao, Krishna V. TI Dynamics of Verticillium Species Microsclerotia in Field Soils in Response to Fumigation, Cropping Patterns, and Flooding SO PHYTOPATHOLOGY LA English DT Article ID SCLEROTINIA-SCLEROTIORUM; INOCULUM DENSITY; DAHLIAE; WILT; CAULIFLOWER; STRAWBERRY; SURVIVAL; LETTUCE; GERMINATION; MANAGEMENT AB Verticillium dahliae is a soilborne, economically significant fungal plant pathogen that persists in the soil for up to 14 years as melanized microsclerotia (ms). Similarly, V longisporum is a very significant production constraint on members of the family Brassicaceae. Management of Verticillium wilt has relied on methods that reduce ms below crop-specific thresholds at which little or no disease develops. Methyl bromide, a broad-spectrum biocide, has been used as a preplant soil fumigant for over 50 years to reduce V dahliae ins. However, reductions in the number of ms in the vertical and horizontal soil profiles and the rate at which soil recolonization occurs has not been studied. The dynamics of ms in soil before and after methyl bromide + chloropicrin fumigation were followed over 3 years in six 8-by-8-m sites in two fields. In separate fields, the dynamics of ms in the 60-cm-deep vertical soil profile pre- and postfumigation with methyl bromide + chloropicrin followed by various cropping patterns were studied over 4 years. Finally, ms densities were assessed in six 8-by-8-m sites in a separate field prior to and following a natural 6-week flood. Methyl bromide + chloripicrin significantly reduced but did not eliminate V dahliae ms in either the vertical or horizontal soil profiles. In field studies, increases in ms were highly dependent upon the crop rotation pattern followed postfumigation. In the vertical soil profile, densities of ms were highest in the top 5 to 20 cm of soil but were consistently detected at 60-cm depths. Six weeks of natural flooding significantly reduced (on average, approximately 65% in the total viable counts of ms) but did not eliminate viable ms of V longisporum. C1 [Short, Dylan P. G.; Gurung, Suraj; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, 1636 E Alisal St, Salinas, CA 93905 USA. [Sandoya, German] Univ Calif Davis, Genome Ctr, Salinas, CA 93905 USA. [Sandoya, German] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Vallad, Gary E.] Univ Florida, IFAS, Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. [Xiao, Chang-Lin] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wu, Bo-Ming] China Agr Univ, Dept Plant Pathol, Beijing 100193, Peoples R China. [Hayes, Ryan J.] USDA ARS, Salinas, CA 93905 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, 1636 E Alisal St, Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 NR 34 TC 2 Z9 2 U1 2 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2015 VL 105 IS 5 BP 638 EP 645 DI 10.1094/PHYTO-09-14-0259-R PG 8 WC Plant Sciences SC Plant Sciences GA CJ2JW UT WOS:000355311500007 PM 25626074 ER PT J AU Novakazi, F Inderbitzin, P Sandoya, G Hayes, RJ von Tiedemann, A Subbarao, KV AF Novakazi, Fluture Inderbitzin, Patrik Sandoya, German Hayes, Ryan J. von Tiedemann, Andreas Subbarao, Krishna V. TI The Three Lineages of the Diploid Hybrid Verticillium longisporum Differ in Virulence and Pathogenicity SO PHYTOPATHOLOGY LA English DT Article ID BRASSICA-NAPUS; SYSTEMIC SPREAD; OILSEED CROP; V-DAHLIAE; PLANT; WILT; DISCOLORATION; HYBRIDIZATION; HORSERADISH; CAULIFLOWER AB Verticillium longisporum is an economically important vascular pathogen of Brassicaceae crops in different parts of the world. V longisporum is a diploid hybrid that consists of three different lineages, each of which originated from a separate hybridization event between two different sets of parental species. We used 20 isolates representing the three V longisporam lineages and the relative V dahliae, and performed pathogenicity tests on 11 different hosts, including artichoke, cabbage, cauliflower, cotton, eggplant, horseradish, lettuce, linseed, oilseed rape (canola), tomato, and watermelon. V longisporum was overall more virulent on the Brassicaceae crops than V dahliae, which was more virulent than V longisporum across the nonBrassicaceae crops. There were differences in virulence between the three V longisporum lineages. V longisporum lineage A1/D1 was the most virulent lineage on oilseed rape, and V longisporum lineage A1/D2 was the most virulent lineage on cabbage and horseradish. We also found that on the nonBrassicaceae hosts eggplant, tomato, lettuce, and watermelon, V longisporum was more or equally virulent than V dahliae. This suggests that V longisporum may have a wider potential host range than currently appreciated. C1 [Novakazi, Fluture; von Tiedemann, Andreas] Univ Gottingen, Plant Pathol & Crop Protect Div, Dept Crop Sci, Fac Agr, D-37077 Gottingen, Germany. [Inderbitzin, Patrik; Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Sandoya, German] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Sandoya, German] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Hayes, Ryan J.] USDA ARS, Salinas, CA 93905 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. EM kvsubbarao@ucdavis.edu OI Subbarao, Krishna/0000-0002-2075-1835 FU USDA-NIFA-SCRI [2010-51181-21069]; California Leafy Greens Research Board FX We thank the following individuals for providing strains used in this research: M. Babadoost (University of Illinois, United States), the late D. Barbara (University of Warwick, United Kingdom), H. Sakai (Department of Agriculture, Gunma Prefecture, Japan), G. Stanosz (University of Wisconsin, United States), M. Typas (University of Athens, Greece), T. Usami (Chiba University, Japan), and I. Happstadius (Lantmannen Lantbruk, Svalov, Sweden); R. M. Davis (UC Davis) for providing cotton seed; R. M. Bostock (UC Davis) for making his lab space available for this work; K. Godfrey and T. Simpson (UC Davis) for help in the greenhouse; S. Herbert, R. Marchebout, K. Maruthachalam, K. McConnell, D. Short, E. Vorbeck, J. Schaper, D. Tacke, and M. Knobel for excellent assistance in the greenhouse and laboratory; and USDA-NIFA-SCRI grant number 2010-51181-21069 and the California Leafy Greens Research Board for the funding that made these studies possible. NR 33 TC 2 Z9 2 U1 2 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2015 VL 105 IS 5 BP 662 EP 673 DI 10.1094/PHYTO-10-14-0265-R PG 12 WC Plant Sciences SC Plant Sciences GA CJ2JW UT WOS:000355311500010 PM 25585057 ER PT J AU Weiland, JE Garrido, P Kamvar, ZN Espindola, AS Marek, SM Grunwald, NJ Garzon, CD AF Weiland, Jerry E. Garrido, Patricia Kamvar, Zhian N. Espindola, Andres S. Marek, Stephen M. Gruenwald, Niklaus J. Garzon, Carla D. TI Population Structure of Pythium irregulare, P. ultimum, and P. sylvaticum in Forest Nursery Soils of Oregon and Washington SO PHYTOPATHOLOGY LA English DT Article ID MULTILOCUS GENOTYPE DATA; APHANIDERMATUM POPULATIONS; GENETIC-VARIATION; GENUS PYTHIUM; METALAXYL SENSITIVITY; INFECTING CUCUMBER; MITOCHONDRIAL-DNA; PACIFIC-NORTHWEST; DIVERSITY; POLYMORPHISMS AB Pythium species are important soilbome pathogens occurring in the forest nursery industry of the Pacific Northwest. However, little is known about their genetic diversity or population structure and it is suspected that isolates are moved among forest nurseries on seedling stock and shared field equipment. In order to address these concerns, a total of 115 isolates of three Pythium species (P. irregulare, P. sylvaticum, and P. ultimum) were examined at three forest nurseries using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Analyses revealed distinct patterns of intraspecific variation for the three species. P. sylvaticum exhibited the most diversity, followed by P. irregulare, while substantial clonality was found in P ultimum. For both P. irregulare and P. sylvaticum, but not P. ultimum, there was evidence for significant variation among nurseries. However, all three species also exhibited at least two distinct lineages not associated with the nursery of origin. Finally, evidence was found that certain lineages and clonal genotypes, including fungicide-resistant isolates, are shared among nurseries, indicating that pathogen movement has occurred. C1 [Weiland, Jerry E.; Gruenwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Weiland, Jerry E.; Kamvar, Zhian N.; Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Garrido, Patricia; Espindola, Andres S.; Garzon, Carla D.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74074 USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. EM weilandj@onid.orst.edu; carla.garzon@okstate.edu RI Espindola, Andres/A-5056-2016; Kamvar, Zhian/C-5236-2017; Marek, Stephen/A-7610-2013; OI Espindola, Andres/0000-0002-9658-0673; Kamvar, Zhian/0000-0003-1458-7108; Marek, Stephen/0000-0002-0146-8049; Grunwald, Niklaus/0000-0003-1656-7602 FU USDA-ARS CRIS [5358-22000-034-00D, 5358-22000-039-00D] FX We thank M. Larsen, J. Tabima, and F. Flores for their excellent technical support; and H. Melouk for reviewing earlier versions of the manuscript. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture (USDA) or the Agricultural Research Service (ARS) of any product or service to the exclusion of others that may be suitable. Financial support was provided by the USDA-ARS CRIS 5358-22000-034-00D and 5358-22000-039-00D. NR 70 TC 1 Z9 1 U1 2 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2015 VL 105 IS 5 BP 684 EP 694 DI 10.1094/PHYTO-05-14-0147-R PG 11 WC Plant Sciences SC Plant Sciences GA CJ2JW UT WOS:000355311500012 PM 25607720 ER PT J AU Kolmer, JA AF Kolmer, J. A. TI Collections of Puccinia triticina in Different Provinces of China Are Highly Related for Virulence and Molecular Genotype SO PHYTOPATHOLOGY LA English DT Article ID LEAF RUST RESISTANCE; F-SP TRITICI; WHEAT CULTIVARS; PHYSIOLOGICAL SPECIALIZATION; GENETIC DIFFERENTIATION; MICROSATELLITE LOCI; NORTH-AMERICA; UNITED-STATES; COMMON WHEAT; POPULATION AB Collections of Puccinia triticina, the wheat leaf rust pathogen, were obtained from seven provinces in China from 2009 and 2010. Single uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ for single leaf rust resistance genes, and for molecular genotype with 23 simple sequence repeat (SSR) primers. Fortyeight virulence phenotypes were described among the 155 isolates tested for virulence. All but four isolates were virulent to Lr26, and no isolates with virulence to Lr18 or Lr24 were found. The three most common phenotypes, FCBQQ, PCGLN, and PCGLL, were found in five, five, and three provinces, respectively. Thirty-six SSR genotypes were found among the 100 isolates tested for molecular variation. Isolates with identical virulence phenotypes and SSR genotypes were found in more than one province. Analysis of variation showed no overall differentiation of SSR genotypes or virulence phenotypes based on province of origin. The SSR genotypes had high levels of linkage disequilibrium, high levels of observed heterozygosity, and significant correlation with the virulence phenotypes, all measures that indicated clonal reproduction. Bayesian cluster analysis and principle component plots indicated three groups of SSR genotypes that also varied significantly for virulence. The seven provinces are continuously adjacent to each other and likely form a single epidemiological zone for P. triticina. C1 [Kolmer, J. A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Jim.Kolmer@ars.usda.gov NR 37 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2015 VL 105 IS 5 BP 700 EP 706 DI 10.1094/PHYTO-11-14-0293-R PG 7 WC Plant Sciences SC Plant Sciences GA CJ2JW UT WOS:000355311500014 PM 25585058 ER PT J AU Park, T AF Park, Timothy TI Direct Marketing and the Structure of Farm Sales: An Unconditional Quantile Regression Approach SO JOURNAL OF AGRICULTURAL AND RESOURCE ECONOMICS LA English DT Article DE direct marketing; influence functions; unconditional quantile regressions ID SUPPLIER ENCROACHMENT; MODELS AB This paper examines the impact of participation in direct marketing on the entire distribution of farm sales using the unconditional quantile regression (UQR) estimator. Our analysis yields unbiased estimates of the unconditional impact of direct marketing on farm sales and reveals the heterogeneous effects that occur across the distribution of farm sales. The impacts of direct marketing efforts are uniformly negative across the UQR results, but declines in sales tend to grow smaller as sales increase. Producers planning to sell more in local outlets should expect sales to decline. Marketing experts and extension professionals can use this information to guide farmers who are considering initiating or expanding direct marketing activities. C1 [Park, Timothy] Econ Res Serv, USDA, Washington, DC 20024 USA. RP Park, T (reprint author), Econ Res Serv, USDA, Washington, DC 20024 USA. NR 28 TC 0 Z9 0 U1 1 U2 5 PU WESTERN AGRICULTURAL ECONOMICS ASSOC PI LOGAN PA C/O DEEVON BAILEY, UTAH STATE UNIV, ECONOMICS DEPT, 3535 OLD MAIN HILL, LOGAN, UT 84322-3530 USA SN 1068-5502 J9 J AGR RESOUR ECON JI J. Agric. Resour. Econ. PD MAY PY 2015 VL 40 IS 2 BP 266 EP 284 PG 19 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DB8GV UT WOS:000368756100005 ER PT J AU Jaradat, AA AF Jaradat, Abdullah A. TI Organic agriculture: The science and practices under a changing climate SO EMIRATES JOURNAL OF FOOD AND AGRICULTURE LA English DT Editorial Material C1 [Jaradat, Abdullah A.] Univ Minnesota, USDA ARS, Minneapolis, MN 55455 USA. [Jaradat, Abdullah A.] Univ Minnesota, Dept Agron & Plant Genet, Minneapolis, MN 55455 USA. RP Jaradat, AA (reprint author), Univ Minnesota, USDA ARS, Minneapolis, MN 55455 USA. EM abdullah.jaradat@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU UNITED ARAB EMIRATES UNIV PI AL AIN PA P. O. BOX 17551, AL AIN, U ARAB EMIRATES SN 2079-052X EI 2079-0538 J9 EMIR J FOOD AGR JI Emir. J. Food Agric. PD MAY PY 2015 VL 27 IS 5 BP 1 EP 2 DI 10.9755/ejfa.2015.04.142 PG 2 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA DB5QW UT WOS:000368569700001 ER PT J AU Gao, Y Faris, JD Liu, Z Kim, YM Syme, RA Oliver, RP Xu, SS Friesen, TL AF Gao, Y. Faris, J. D. Liu, Z. Kim, Y. M. Syme, R. A. Oliver, R. P. Xu, S. S. Friesen, T. L. TI Identification and Characterization of the SnTox6-Snn6 Interaction in the Parastagonospora nodorum-Wheat Pathosystem SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID HOST-SELECTIVE TOXINS; EFFECTOR-TRIGGERED SUSCEPTIBILITY; PYRENOPHORA-TRITICI-REPENTIS; STAGONOSPORA-NODORUM; WINTER-WHEAT; SEPTORIA-NODORUM; LEAF BLOTCH; PTR TOXA; GENE; RESISTANCE AB Parastagonospora nodorum is a necrotrophic fungal pathogen that causes Septoria nodorum blotch (SNB) (formerly Stagonospora nodorum blotch) on wheat. P. nodorum produces necrotrophic effectors (NE) that are recognized by dominant host sensitivity gene products resulting in disease development. The NE-host interaction is critical to inducing NE-triggered susceptibility (NETS). To date, seven NE-host sensitivity gene interactions, following an inverse gene-for-gene model, have been identified in the P. nodorum-wheat pathosystem. Here, we used a wheat mapping population that segregated for sensitivity to two previously characterized interactions (SnTox1-Snn1 and SnTox3-Snn3-B1) to identify and characterize a new interaction involving the NE designated SnTox6 and the host sensitivity gene designated Snn6. SnTox6 is a small secreted protein that induces necrosis on wheat lines harboring Snn6. Sensitivity to SnTox6, conferred by Snn6, was light-dependent and was shown to underlie a major disease susceptibility quantitative trait locus (QTL). No other QTL were identified, even though the P. nodorum isolate used in this study harbored both the SnTox1 and SnTox3 genes. Reverse transcription-polymerase chain reaction showed that the expression of SnTox1 was not detectable, whereas SnTox3 was expressed and, yet, did not play a significant role in disease development. This work expands our knowledge of the wheat-P. nodorum interaction and further establishes this system as a model for necrotrophic specialist pathosystems. C1 [Gao, Y.; Liu, Z.; Kim, Y. M.; Friesen, T. L.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Faris, J. D.; Xu, S. S.; Friesen, T. L.] USDA ARS, No Crop Sci Lab, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Syme, R. A.; Oliver, R. P.] Curtin Univ, Ctr Crop & Dis Management, Dept Environm & Agr, Perth, WA, Australia. RP Friesen, TL (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. EM timothy.friesen@ars.usda.gov FU U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture-Agriculture and Food Research Initiative Microbial Biology Program [2010-65108-20543]; USDA Agricultural Research Service Current Research Information System Projects [5442-22000-048-00D, 5442-22000-037-00D] FX This project was funded by the U.S. Department of Agriculture (USDA) National Institute of Food and Agriculture-Agriculture and Food Research Initiative Microbial Biology Program grant number 2010-65108-20543 and USDA Agricultural Research Service Current Research Information System Projects 5442-22000-048-00D and 5442-22000-037-00D. A special thanks to L. Higgins and T. Markowski, Center for Mass Spectrometry and Proteomics, University of Minnesota, for guidance with MS analysis. We thank D. Holmes for technical assistance and R. Brueggeman and M. Bolton for their critical reviews. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. USDA is an equal opportunity provider and employer. NR 52 TC 11 Z9 11 U1 2 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD MAY PY 2015 VL 28 IS 5 BP 615 EP 625 DI 10.1094/MPMI-12-14-0396-R PG 11 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA CJ2NY UT WOS:000355322900010 PM 25608181 ER PT J AU Dowd, PF Johnson, ET AF Dowd, Patrick F. Johnson, Eric T. TI Environmental effects on resistance gene expression in milk stage popcorn kernels and associations with mycotoxin production SO MYCOTOXIN RESEARCH LA English DT Article DE Popcorn; Mycotoxin; Plant resistance; Insect pest; Environmental stress ID ASPERGILLUS-FLAVUS INFECTION; MAIZE WEEVIL RESISTANCE; INSECT RESISTANCE; DROUGHT STRESS; INDIRECT REDUCTION; PLANT DEFENSE; EAR MOLDS; CORN; IDENTIFICATION; HERBIVORES AB Like other forms of maize, popcorn is subject to increased levels of contamination by a variety of different mycotoxins under stress conditions, although levels generally are less than dent maize under comparable stress. Gene array analysis was used to determine expression differences of disease resistance-associated genes in milk stage kernels from commercial popcorn fields over 3 years. Relatively lower expression of resistance gene types was noted in years with higher temperatures and lower rainfall, which was consistent with prior results for many previously identified resistance response-associated genes. The lower rates of expression occurred for genes such as chitinases, protease inhibitors, and peroxidases; enzymes involved in the synthesis of cell wall barriers and secondary metabolites; and regulatory proteins. However, expression of several specific resistance genes previously associated with mycotoxins, such as aflatoxin in dent maize, was not affected. Insect damage altered the spectrumof resistance gene expression differences compared to undamaged ears. Correlation analyses showed expression differences of some previously reported resistance genes that were highly associated with mycotoxin levels and included glucanases, protease inhibitors, peroxidases, and thionins. C1 [Dowd, Patrick F.; Johnson, Eric T.] ARS, Crop BioProtect Res Unit, USDA, Peoria, IL 61604 USA. RP Dowd, PF (reprint author), ARS, Crop BioProtect Res Unit, USDA, Peoria, IL 61604 USA. EM Patrick.Dowd@ars.usda.gov OI Johnson, Eric/0000-0002-1665-6229 NR 51 TC 1 Z9 1 U1 2 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0178-7888 EI 1867-1632 J9 MYCOTOXIN RES JI Mycotoxin Res. PD MAY PY 2015 VL 31 IS 2 BP 63 EP 82 DI 10.1007/s12550-014-0215-1 PG 20 WC Mycology; Toxicology SC Mycology; Toxicology GA CS4XT UT WOS:000362080300001 PM 25512225 ER PT J AU Wisniewski, M Neuner, G Gusta, LV AF Wisniewski, Michael Neuner, Gilbert Gusta, Lawrence V. TI The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 99; Freeze avoidance; supercooling; ice nucleation active bacteria; frost tolerance; ice crystallization; antifreeze proteins; intrinsic nucleation; extrinsic nucleation; heterogeneous nucleation; homogeneous nucleation; differential thermal analysis ID THERMAL-ANALYSIS IDTA; HIGH-MOUNTAIN PLANTS; FROST-RESISTANCE; VIDEO THERMOGRAPHY; FIELD CONDITIONS; TEMPERATURE; EVENTS; LEAVES; MICROSCOPY; CLIMATE AB Freezing events that occur when plants are actively growing can be a lethal event, particularly if the plant has no freezing tolerance. Such frost events often have devastating effects on agricultural production and can also play an important role in shaping community structure in natural populations of plants, especially in alpine, sub-arctic, and arctic ecosystems. Therefore, a better understanding of the freezing process in plants can play an important role in the development of methods of frost protection and understanding mechanisms of freeze avoidance. Here, we describe a protocol to visualize the freezing process in plants using high-resolution infrared thermography (HRIT). The use of this technology allows one to determine the primary sites of ice formation in plants, how ice propagates, and the presence of ice barriers. Furthermore, it allows one to examine the role of extrinsic and intrinsic nucleators in determining the temperature at which plants freeze and evaluate the ability of various compounds to either affect the freezing process or increase freezing tolerance. The use of HRIT allows one to visualize the many adaptations that have evolved in plants, which directly or indirectly impact the freezing process and ultimately enables plants to survive frost events. C1 [Wisniewski, Michael] ARS, USDA, Kearneysville, WV USA. [Neuner, Gilbert] Univ Innsbruck, Inst Bot, A-6020 Innsbruck, Austria. [Gusta, Lawrence V.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 0W0, Canada. RP Wisniewski, M (reprint author), ARS, USDA, Kearneysville, WV USA. EM Michael.Wisniewski@ars.usda.gov FU Austrian Science Fund (FWF) [P23681-B16] FX This research was funded by the Austrian Science Fund (FWF): P23681-B16. NR 38 TC 0 Z9 0 U1 3 U2 12 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD MAY PY 2015 IS 99 AR e52703 DI 10.3791/52703 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CR7MV UT WOS:000361535300042 PM 25992743 ER PT J AU Lu, YJ Fuerst, EP Lv, JL Morris, CF Yu, L Fletcher, A Kiszonas, AM Yu, LL Luthria, D AF Lu, Yingjian Fuerst, E. Patrick Lv, Junli Morris, Craig F. Yu, Lu Fletcher, Arnetta Kiszonas, Alecia M. Yu, Liangli Luthria, Devanand TI Phytochemical Profile and Antiproliferative Activity of Dough and Bread Fractions Made from Refined and Whole Wheat Flours SO CEREAL CHEMISTRY LA English DT Article ID PHENOLIC-ACIDS; ANTIOXIDANT PROPERTIES; BAKERY PRODUCTS; HEART-DISEASE; DIETARY FIBER; RYE BREAD; VITAMIN-E; CAROTENOIDS; FOODS; TOCOPHEROL AB Phytochemical profile (phenolic acids, carotenoids, and tocopherols) and antiproliferative properties of bread processing fractions, including the dough, crumb, and upper crust made from refined wheat and whole wheat flours were analyzed for two wheat cultivars. Ferulic acid, lutein, and alpha-tocopherol were the predominant phenolic acid, carotenoid, and tocopherol, respectively, extracted from all fractions. The levels of all phytochemicals in whole wheat samples were over eightfold higher than their corresponding refined wheat samples. The concentrations of total phenolic acids (soluble and insoluble bound) were higher in the upper crust of refined (similar to 60-90%) and whole wheat (similar to 15-40%) breads than their corresponding dough fractions. However, the dough of whole wheat had higher levels of tocopherols and carotenoids compared with the crumb and upper crust, suggesting that phenolic acids were relatively stable during baking, whereas tocopherols (similar to 25-80%) and carotenoids (similar to 20-80%), were partially degraded. The antiproliferative activity of whole wheat bread extracts against HT-29 cancer cells was weakly correlated with total phenolic acids but showed no correlations with total carotenoid and total tocopherol contents. C1 [Lu, Yingjian; Lv, Junli; Yu, Lu; Fletcher, Arnetta; Yu, Liangli] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Lu, Yingjian; Luthria, Devanand] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Fuerst, E. Patrick] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Fuerst, E. Patrick; Morris, Craig F.; Kiszonas, Alecia M.] ARS, Western Wheat Qual Lab, USDA, Pullman, WA 99164 USA. [Lv, Junli] Inner Mongolia Univ Sci & Technol, Sch Math Phys & Biol Engn, Baotou 014010, Inner Mongolia, Peoples R China. RP Luthria, D (reprint author), ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Dave.Luthria@ars.usda.gov FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2009-02347] FX The authors acknowledge Samina Shami of USDA-ARS for her assistance in carrying out experimentation. This project was supported by the Agriculture and Food Research Initiative grant no. 2009-02347 from the USDA National Institute of Food and Agriculture. NR 28 TC 0 Z9 0 U1 3 U2 15 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2015 VL 92 IS 3 BP 271 EP 277 DI 10.1094/CCHEM-10-14-0214-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK3CR UT WOS:000356094100007 ER PT J AU Park, EY Baik, BK Machado, S Gollany, HT Fuerst, EP AF Park, Eun Young Baik, Byung-Kee Machado, Stephen Gollany, Hero T. Fuerst, E. Patrick TI Functional and Nutritional Characteristics of Soft Wheat Grown in No-Till and Conventional Cropping Systems SO CEREAL CHEMISTRY LA English DT Article ID SOLVENT RETENTION CAPACITIES; FED MEDITERRANEAN CONDITIONS; SNAP COOKIE QUALITY; LONG-TERM TILLAGE; RED WINTER-WHEAT; SPRING WHEAT; ANTIOXIDANT ACTIVITY; NITROGEN-FERTILIZATION; RHEOLOGICAL PROPERTIES; YIELD COMPONENTS AB The effects of no-till versus conventional farming practices were evaluated on soft wheat functional and nutritional characteristics, including kernel physical properties, whole wheat composition, antioxidant activity, and end-product quality. Soft white winter wheat cultivar ORCF 102 was evaluated over a two-year period from three long-term replicated no-till versus conventional tillage studies in Oregon. Wheat from the no-till cropping systems generally had greater test weight, kernel diameter, and kernel weight and had softer kernels compared with wheat from the conventional tillage systems. Compared with the conventional systems, no-till whole wheat flour had lower protein and SDS sedimentation volume. Ash content as well as most minerals measured (calcium, copper, iron, magnesium, and zinc), except for manganese and phosphorus, were generally slightly lower in no-till than in conventional wheat. Whole wheat flour from the no-till cropping systems generally had slightly lower total phenolic content and total antioxidant capacity. Milling properties, including flour yield, break flour yield, and mill score, were not affected by tillage systems. Refined flour from no-till systems had lower protein, SDS sedimentation volume, and lactic acid and sucrose solvent retention capacities compared with flour from conventional tillage. No-till wheat generally had greater sugar-snap cookie diameter than conventionally tilled wheat. In conclusion, no-till soft white winter wheat generally had slightly reduced nutritional properties (protein, ash, most minerals, and total antioxidant content) compared with wheat from conventionally tilled systems, and it had equivalent or sometimes superior functional properties for baking cookie-type products. C1 [Park, Eun Young] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Baik, Byung-Kee] ARS, USDA, CSWQRU, Wooster, OH 44691 USA. [Machado, Stephen] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. [Gollany, Hero T.] ARS, USDA, CPCRC, Adams, OR 97810 USA. [Fuerst, E. Patrick] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Fuerst, EP (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM pfuerst@wsu.edu FU Washington State University's Center for Sustaining Agriculture and Natural Resources; Shepherd's Grain FX This work was supported in part by the BioAg program of Washington State University's Center for Sustaining Agriculture and Natural Resources and by Shepherd's Grain. We thank Larry Pritchett for technical assistance with the Oregon trials. We thank Wayne Polumsky at USDA-ARS-CPCRC for technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 59 TC 2 Z9 2 U1 2 U2 10 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2015 VL 92 IS 3 BP 332 EP 338 DI 10.1094/CCHEM-09-14-0184-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CK3CR UT WOS:000356094100015 ER PT J AU Ehlenfeldt, MK Rowland, LJ Ogden, EL Vinyard, BT AF Ehlenfeldt, Mark K. Rowland, Lisa J. Ogden, Elizabeth L. Vinyard, Bryan T. TI LT25, LT50, and LT75 floral bud cold hardiness determinations for a diverse selection of Vaccinium genotypes SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Acclimation; deacclimation; dormant AB Freeze-bath cold hardiness results were used to calculate LT25 and LT75 values (temperatures causing 25% and 75% lethality, respectively) for a diverse selection of Vaccinium genotypes which had been previously analyzed for LT50 values. Overall, LT25 and LT75 values paralleled the LT50 values. Across genotypes, the LT25 was approximately 1.7 degrees C (3.1 degrees F) higher than the LT50, and the LT75 approximately 1.6 degrees C (2.9 degrees F) lower than the LT50 for any given date. C1 [Ehlenfeldt, Mark K.] USDA ARS, Marucci Ctr Blueberry & Cranberry Res & Extens, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ 08019 USA. [Rowland, Lisa J.; Ogden, Elizabeth L.] USDA ARS, Beltsville Agr Res Ctr, GIFVL, Beltsville, MD 20705 USA. [Vinyard, Bryan T.] USDA ARS, Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. RP Ehlenfeldt, MK (reprint author), USDA ARS, Marucci Ctr Blueberry & Cranberry Res & Extens, Genet Improvement Fruits & Vegetables Lab, 125A Lake Oswego Rd, Chatsworth, NJ 08019 USA. EM mark.ehlenfeldt@ars.usda.gov NR 1 TC 0 Z9 0 U1 3 U2 6 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD MAY PY 2015 VL 95 IS 3 BP 491 EP 494 DI 10.4141/CJPS-2014-350 PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CR0FB UT WOS:000360992700005 ER PT J AU Lutomiah, J Omondi, D Masiga, D Mutai, C Mireji, PO Ongus, J Linthicum, KJ Sang, R AF Lutomiah, Joel Omondi, David Masiga, Daniel Mutai, Collins Mireji, Paul O. Ongus, Juliette Linthicum, Ken J. Sang, Rosemary TI Blood meal analysis and virus detection in blood-fed mosquitoes collected during the 2006-2007 Rift Valley fever outbreak in Kenya SO GENOME LA English DT Meeting Abstract C1 [Lutomiah, Joel; Mutai, Collins; Sang, Rosemary] Kenya Med Res Inst KEMRI, Nairobi, Kenya. [Omondi, David; Masiga, Daniel] Int Ctr Insect Physiol & Ecol, Nairobi, Kenya. [Mireji, Paul O.] Egerton Univ, Dept Biochem & Mol Biol, Njoro, Kenya. [Ongus, Juliette] Jomo Kenyatta Univ Agr & Technol, Ruiru, Kenya. [Linthicum, Ken J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. EM domondi@icipe.org NR 0 TC 0 Z9 0 U1 1 U2 2 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD MAY PY 2015 VL 58 IS 5 BP 249 EP 249 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CQ8MF UT WOS:000360861200241 ER PT J AU Rowe, G Griswold, T Packer, L AF Rowe, Genevieve Griswold, Terry Packer, Laurence TI Integrative taxonomy uncovers hidden diversity within three genera of Canadian Osmiini (Hymenoptera: Megachilidae) SO GENOME LA English DT Meeting Abstract C1 [Rowe, Genevieve; Packer, Laurence] York Univ, Toronto, ON M3J 1P3, Canada. [Griswold, Terry] USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. EM genevieve.rowe@ymail.com NR 0 TC 0 Z9 0 U1 1 U2 1 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD MAY PY 2015 VL 58 IS 5 BP 273 EP 273 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CQ8MF UT WOS:000360861200331 ER PT J AU Rojas, ES Batzer, JC Beattie, GA Fleischer, SJ Shapiro, LR Williams, MA Bessin, R Bruton, BD Boucher, TJ Jesse, LCH Gleason, ML AF Rojas, Erika Saalau Batzer, Jean C. Beattie, Gwyn A. Fleischer, Shelby J. Shapiro, Lori R. Williams, Mark A. Bessin, Ricardo Bruton, Benny D. Boucher, T. Jude Jesse, Laura C. H. Gleason, Mark L. TI Bacterial Wilt of Cucurbits: Resurrecting a Classic Pathosystem SO PLANT DISEASE LA English DT Article ID STRIPED CUCUMBER BEETLE; ACALYMMA-VITTATUM COLEOPTERA; GROWTH-PROMOTING RHIZOBACTERIA; ERWINIA-TRACHEIPHILA; F COLEOPTERA; ROW COVERS; CHRYSOMELIDAE DENSITY; AGGREGATION PHEROMONE; BIOLOGICAL-CONTROL; BUTTERNUT SQUASH C1 [Rojas, Erika Saalau] Univ Massachusetts, Amherst, MA 01003 USA. [Batzer, Jean C.; Beattie, Gwyn A.; Jesse, Laura C. H.; Gleason, Mark L.] Iowa State Univ, Ames, IA 50011 USA. [Fleischer, Shelby J.] Penn State Univ, University Pk, PA 16802 USA. [Shapiro, Lori R.] Harvard Univ, Cambridge, MA 02138 USA. [Williams, Mark A.; Bessin, Ricardo] Univ Kentucky, Lexington, KY USA. [Bruton, Benny D.] USDA ARS, Lane, OK USA. [Boucher, T. Jude] Univ Connecticut, Vernon, CT USA. RP Gleason, ML (reprint author), Iowa State Univ, Ames, IA 50011 USA. EM mgleason@iastate.edu NR 99 TC 3 Z9 3 U1 5 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 564 EP 574 DI 10.1094/PDIS-10-14-1068-FE PG 11 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800001 ER PT J AU Haudenshield, JS Hartman, GL AF Haudenshield, James S. Hartman, Glen L. TI Archaeophytopathology of Phakopsora pachyrhizi, the Soybean Rust Pathogen SO PLANT DISEASE LA English DT Article ID ASSAYS AB Herbarium specimens are useful to compare attributes of the past to attributes of today and predictions into the future. In this study, herbarium specimens from 1887 to 2006 were used to identify Phakopsora pachyrhizi and P. meibomiae, the two known fungal species that cause soybean lust. Historically, these two species differed in geographic distribution, with P. pachyrhizi confined to Asia and Australia, and P. meibonziae confined to the Americas. In our analyses, herbarium specimens were used to determine whether it was possible to extract adequate useful DNA from the fungal structures. If present, quantitative PCR primers specific to P. pachyrhizi, P. meibomiae, or to a third group inclusive of many rust species could be used to speciate the fungus. Of the 38 archival specimens, 11 were positive for P. pachyrhizi, including a 1912 specimen from Japan; 15 were positive for P. meibomiae, including a 1928 specimen from Brazil and two 1923 specimens from the Philippines; and 12 (including all African accessions) were negative for both species. Five specimens were positive in the more inclusive rust assay; all had been labeled as P. pachyrhizi and none were on soybean. These results demonstrate the feasibility of DNA genotyping in archaeophytopathological investigations. C1 [Haudenshield, James S.; Hartman, Glen L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Haudenshield, James S.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM ghartman@illinois.edu NR 13 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 575 EP 579 DI 10.1094/PDIS-07-14-0772-SR PG 5 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800002 ER PT J AU Sharma-Poudyal, D Paulitz, TC Porter, LD du Toit, LJ AF Sharma-Poudyal, Dipak Paulitz, Timothy C. Porter, Lyndon D. du Toit, Lindsey J. TI Characterization and Pathogenicity of Rhizoctonia and Rhizoctonia-Like spp. From Pea Crops in the Columbia Basin of Oregon and Washington SO PLANT DISEASE LA English DT Article ID PREEMERGENCE DAMPING-OFF; ROOT-ROT; BINUCLEATE RHIZOCTONIA; INOCULUM DENSITY; SOIL-TEMPERATURE; NORTH-DAKOTA; SOLANI AG-8; WHEAT; VIRULENCE; CANOLA AB Isolates of Rhizoctonia and Rhizoctonia-like spp. (n = 179) were baited selectively from soil and plant samples collected from irrigated pea crops in the semiarid Columbia Basin of Oregon and Washington from 2011 to 2013, and characterized to species, subspecies, and anastomosis groups (AG) based on sequences of the internal transcribed spacer region of ribosomal DNA. Rhizoctonia solani comprised 76% of all isolates, and included isolates of AG 4(31% of all isolates), AG 2-1 (18%), AG 3 (10%), AG 8 (8%), AG 5 (5%), AG 10 (3%), and AG 9 (1%). The isolates of Ceratobasidium spp. (20%) comprised four AGs: AG K (11%), AG A (6%), AG I (2%), and AG I-like (1%). Waitea circinata isolates (4%) comprised two subspecies: W. circinata var. circinata (approximately 4%) and W. circinata var. zeae (<1%). Repeated pathogenicity tests of isolates of the 10 most frequently detected AGs and subspecies on 'Serge' pea at 15 degrees C revealed that R. solani AG 2-1 caused the greatest reduction in pea emergence, followed by R. solani AG 4. R. solani AG 4 caused the most severe root rot, stunting, and reduction in pea seedling biomass, followed by isolates of AG 2-1. R. solani AG 8 did not affect emergence, plant height, and total biomass compared with noninoculated control plants; however, root rot caused by isolates of AG 8 was ranked the third most severe among isolates of the 10 Rhizoctonia subgroups, after that caused by isolates of AG 4 and AG 2-1. Isolates of other AGs and subspecies were either weakly virulent or nonpathogenic on pea. The most common AGs (AG 4 and AG 2-1) detected in pea fields in the Columbia Basin were also the most virulent. In a growers' pea crop grown for seed (Prevail') planted 5 days after herbicide application and incorporation of a preceding winter wheat crop, severe stunting caused by Rhizoctonia spp. resulted in an average 75% yield loss within patches of stunted plants. In contrast, the yield of processing pea from a green pea crop of Serge did not differ significantly for plants sampled within versus outside patches of stunted plants; however, plants within patches were significantly more mature. In the Prevail seed crop, a greater frequency of R. solani AG 8 was detected than AG 2-1 or AG 4 from within patches of stunted plants, indicating that isolates of AG 8 may be associated with the root rot complex in some pea crops in the Columbia Basin. C1 [Sharma-Poudyal, Dipak] Washington State Univ, Pullman, WA 99164 USA. [Paulitz, Timothy C.] USDA ARS, Pullman, WA 99164 USA. [Porter, Lyndon D.] USDA ARS, Prosser, WA 99350 USA. [du Toit, Lindsey J.] Washington State Univ, Northwestern Washington Res & Extens Ctr, Mt Vernon, WA 98273 USA. RP du Toit, LJ (reprint author), Washington State Univ, Northwestern Washington Res & Extens Ctr, Mt Vernon, WA 98273 USA. EM dutoit@wsu.edu OI Paulitz, Timothy/0000-0002-8885-3803 FU Washington State Department of Agriculture Specialty Crop Block Grant [K525]; PPNS [0666]; Department of Plant Pathology, College of Agricultural, Human, & Natural Resource Sciences, Agricultural Research Center Hatch [WPN05595]; Washington State University [Pullman 99164-6430] FX Washington State Department of Agriculture Specialty Crop Block Grant Number K525 funded this research. The project was also supported by PPNS Number 0666, Department of Plant Pathology, College of Agricultural, Human, & Natural Resource Sciences, Agricultural Research Center Hatch Project Number WPN05595, Washington State University, Pullman 99164-6430. NR 62 TC 3 Z9 3 U1 4 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 604 EP 613 DI 10.1094/PDIS-08-14-0803-RE PG 10 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800006 ER PT J AU Samac, DA Schraber, S Barclay, S AF Samac, Deborah A. Schraber, Samuel Barclay, Stuart TI A Mineral Seed Coating for Control of Seedling Diseases of Alfalfa Suitable for Organic Production Systems SO PLANT DISEASE LA English DT Article ID F-SP-MEDICAGINIS; APHANOMYCES-EUTEICHES; PHYTOPHTHORA-MEGASPERMA; OOSPORE GERMINATION; UNITED-STATES; RESISTANCE; METALAXYL; SURVIVAL; SOIL; NODULATION AB Most alfalfa seed is treated with the fungicide mefenoxam (Apron XL) for control of soilborne seedling diseases caused by Phytophthora medica-ginis and Pythium spp. However, Apron XL is not active against Aphanomyces euteiches, the causal agent of Aphanomyces root rot (ARR), an important component of the alfalfa seedling root rot complex. Moreover, Apron XL-treated seed cannot be used in organic production systems. A seed coating using aluminosilicate (natural zeolite) at a rate of 0.33 g of zeolite per gram of alfalfa seed was tested as an alfalfa seed treatment. Inoculated growth chamber trials were conducted to determine the percentage of seedlings protected from Phytophthora root rot (PER) and ARR. The mineral seed coating resulted in significantly greater control of PRR, with a mean of 89% healthy seedlings (disease score of 1 or 2 on a 1-to-5 scale) compared with the Apron XL treatment, with a mean of 38% healthy seedlings, or the control treatment, with 15% healthy seedlings. The mineral seed coating also resulted in significantly greater protection against ARE. with 67% healthy seedlings compared with 3 and 2% healthy seedlings with the Apron XL and control treatments, respectively. The coated seed were used for in vitro assays with Pythium ultimum and P. paroecandrum to test for protection from seed rot and damping off. The mineral seed coating resulted in a significantly greater percentage of healthy seedlings compared with the Apron XL and control treatments. In growth chamber assays with naturally infested field soils with a range of disease pressure, the mineral seed coating resulted in a similar or greater percentage of healthy plants than the Apron XL treatment. The mineral coating had no effect on in vitro growth of Sinorhizobium meliloti, and nodule numbers were similar on roots from mineral-coated and untreated seed. These experiments indicate that the zeolite seed coating is a promising means of controlling seedling diseases in alfalfa production systems. C1 [Samac, Deborah A.] Univ Minnesota, USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [Samac, Deborah A.; Schraber, Samuel] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Barclay, Stuart] Summit Seed Coatings, Caldwell, ID 83605 USA. RP Samac, DA (reprint author), Univ Minnesota, USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. EM debby.samac@ars.usda.gov NR 38 TC 0 Z9 0 U1 11 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 614 EP 620 DI 10.1094/PDIS-03-14-0240-RE PG 7 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800007 ER PT J AU Nelson, ME Gent, DH Grove, GG AF Nelson, Mark E. Gent, David H. Grove, Gary G. TI Meta-Analysis Reveals a Critical Period for Management of Powdery Mildew on Hop Cones SO PLANT DISEASE LA English DT Article ID INDIVIDUAL PATIENT DATA; PARTICIPANT DATA; HUMULUS-LUPULUS; META-REGRESSION; DOWNY MILDEW; BERRIES; LEAVES AB Results of 28 field trials conducted over a 12-year period investigating management of hop powdery mildew caused by Podosphaera macularis were quantitatively summarized by meta-analysis to compare product efficacy and use patterns by mode of action as defined by Fungicide Resistance Action Committee (FRAC) groups. Availability of original observations enabled individual participant data meta-analysis. Differences in control of powdery mildew on leaves and cones were apparent among fungicide FRAC groups when individual products were evaluated over the course of a growing season. FRAC groups 13, 3, and U13 provided the most efficacious control of powdery mildew on leaves. Percent disease control on cones was influenced by midseason foliar disease and fungicide mode-of-action. FRAC 13 provided significantly better disease control on cones than all other groups except U13, 3, and premixes of 7 with 11. Disease control on leaves was similar when a rotational program of fungicides was used, independent of the modes of action, but improved on cones if FRAC groups 13 and 3 were both included compared with programs consisting of FRAC groups 11 and 3, 11 and 5, or 3 and 5. Disease control on cones was improved from 32 to 52%, on average, when the fungicide quinoxyfen (FRAC 13) was applied at least once during the early stages of cone development, defined in this analysis as 20 July to 10 August, as compared with all other treatments. Efficacy of disease control on cones by quinoxyfen was moderated by and interacted with the incidence of leaves with powdery mildew. Disease control on cones was further improved if two applications of quinoxyfen were made during this period. Collectively, these findings suggest that disease control during juvenile stages of cone development largely influences the success of fungicide programs and point to the critical importance of focusing management efforts during this stage of development, independent of what actual management strategy is employed. C1 [Nelson, Mark E.; Grove, Gary G.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Gent, David H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Nelson, ME (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. EM mark_nelson@wsu.edu FU Washington State University Agriculture Research Center (ARC); Hop Research Council; Washington Hop Commission; Washington State Commission on Pesticide Registration; USDA-ARS [CRIS 5358-21000-040-00]; USDA Specialty Crops Research Initiative FX Financial support for this research was provided by Washington State University Agriculture Research Center (ARC), Hop Research Council, Washington Hop Commission, Washington State Commission on Pesticide Registration, numerous fungicide manufacturers, USDA-ARS CRIS 5358-21000-040-00, and USDA Specialty Crops Research Initiative. We thank P. Ojiambo, W. Mahaffee, and L. Madden for review of an earlier draft of the article; L. Madden for his guidance on statistical analyses; the senior editor and two anonymous reviewers for helpful suggestions that improved this article; and our technical and field support staffs for their work over the past decade that made this research possible. NR 40 TC 8 Z9 8 U1 2 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 632 EP 640 DI 10.1094/PDIS-04-14-0396-RE PG 9 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800010 ER PT J AU Sharma-Poudyal, D Paulitz, TC du Toit, LJ AF Sharma-Poudyal, Dipak Paulitz, Timothy C. du Toit, Lindsey J. TI Stunted Patches in Onion Bulb Crops in Oregon and Washington: Etiology and Yield Loss SO PLANT DISEASE LA English DT Article ID RHIZOCTONIA BARE PATCH; ROOT-ROT; ANASTOMOSIS GROUPS; PACIFIC-NORTHWEST; HOST-SPECIFICITY; CROPPING SYSTEMS; SOLANI AG-8; R-SOLANI; SOIL; WHEAT AB Onion stunting caused by Rhizoctonia spp. is an important soilborne disease on very sandy soils in the Columbia Basin of Oregon and Washington. From 2010 to 2013, 251 isolates of Rhizoctonia or Rhizoctonia-like spp. were obtained from soil and onion plant samples collected from inside and outside patches of stunted plants in 29 onion fields in the Columbia Basin. Sequence analysis of the internal transcribed spacer (ITS) region was used to identify the isolates, with 13 anastomosis groups (AGs) or subspecies detected. The most frequent was Waitea circinata var. circinata (25%), followed by Rhizoctonia solani AG 3 (17%), R. solani AG 4 (14%), Ceratobasidium sp. AG A (10%), R. solani AG 8 (7%), Ceratobasidium sp. AG K (6%), R. solani AG 2-1 (6%), W. circinata var. zeae (6%), R. solani AG 5 (4%), Ceratobasidium sp. AG G (2%), R. solani AG 11(2%), and R. solani AG 1-1B and AG 10 (each <1%). However, the distribution of AGs and subspecies varied depending on whether soil or onion plants samples were collected within or adjacent to patches of stunted onion plants. In an attempt to predict the risk of onion stunting for a field prior to planting, DNA concentrations of AG 2-1, AG 3, AG 4, and AG 8 were quantified from bulk soil samples collected from each of nine growers' fields approximately 1 month before onion sowing in 2012. The preplant DNA concentrations did not show a significant association with the amount of stunting observed in the fields during the growing season. In contrast, the frequency of isolation and DNA concentration of R. solani AG 8 detected in soil samples collected during the growing season were greater from inside patches of stunted onion plants than from adjacent healthy areas of an onion crop sampled in 2012, but not for soil samples collected similarly from an onion crop in 2013. AG 2-1, AG 3, and AG 4 DNA concentrations did not differ significantly in soil sampled inside versus outside stunted patches in the fields sampled in 2012 and 2013. Relationships between the number of bulbs harvested or bulb weight versus severity of stunting were defined using correlation and regression analyses for six onion cultivars grown in seven fields surveyed in 2012 and 2013. Onion stunting reduced the average marketable bulb yield by 25 to 60% within stunted patches of the six cultivars. Stunting did not reduce onion plant stand but consistently reduced the size of bulbs, and yield reduction increased with increasing disease severity. C1 [Sharma-Poudyal, Dipak] Washington State Univ, Pullman, WA 99164 USA. [Paulitz, Timothy C.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [du Toit, Lindsey J.] Washington State Univ, NWREC, Mt Vernon, WA 98273 USA. RP du Toit, LJ (reprint author), Washington State Univ, NWREC, Mt Vernon, WA 98273 USA. EM dutoit@wsu.edu OI Paulitz, Timothy/0000-0002-8885-3803 FU Washington State Department of Agriculture Specialty Crop Block Grant Program [K525]; CRIS Project [WPN05595]; Washington State University (WSU) College of Agricultural, Human, & Natural Resource Sciences; WSU Agricultural Research Center; WSU Department of Plant Pathology (PPNS) [0652] FX This research was supported by the Washington State Department of Agriculture Specialty Crop Block Grant Program (Grant Agreement No. K525); CRIS Project WPN05595; and Washington State University (WSU) College of Agricultural, Human, & Natural Resource Sciences, WSU Agricultural Research Center, and WSU Department of Plant Pathology (PPNS number 0652). We thank M. Handiseni for collection of Rhizoctonia spp. isolates in 2010; K. Schroeder and A. Prescott for technical assistance in the lab; E. Thomas, J. Eggers, and S. Kandel for assistance in the fields; and Drs. David M. Weller and Dennis A. Johnson for reviewing the manuscript. We are thankful to onion grower-cooperators in the Columbia Basin who allowed us to survey their onion fields. NR 53 TC 2 Z9 2 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 648 EP 658 DI 10.1094/PDIS-05-14-0441-RE PG 11 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800012 ER PT J AU Saville, A Graham, K Grunwald, NJ Myers, K Fry, WE Ristaino, JB AF Saville, Amanda Graham, Kim Gruenwald, Niklaus J. Myers, Kevin Fry, William E. Ristaino, Jean Beagle TI Fungicide Sensitivity of US Genotypes of Phytophthora infestans to Six Oomycete-Targeted Compounds SO PLANT DISEASE LA English DT Article ID METALAXYL-RESISTANT STRAINS; EASTERN UNITED-STATES; MEFENOXAM SENSITIVITY; CLONAL LINEAGES; LATE BLIGHT; IN-VITRO; POPULATIONS; POTATO; PATHOGEN; AGGRESSIVENESS AB Phytophthora infestans causes potato late blight, an important and costly disease of potato and tomato crops. Seven clonal lineages of P. infestans identified recently in the United States were tested for baseline sensitivity to six oomycete-targeted fungicides. A subset of the dominant lineages (n = 45) collected between 2004 and 2012 was tested in vitro on media amended with a range of concentrations of either azoxystrobin, cyazofamid, cymoxanil, fluopicolide, mandipropamid, or mefenoxam. Dose-response curves and values for the effective concentration at which 50% of growth was suppressed were calculated for each isolate. The US-8 and US-11 clonal lineages were insensitive to mefenoxam while the US-20, US-21, US-22, US-23, and US-24 clonal lineages were sensitive to mefenoxam. Insensitivity to azoxystrobin, cyazofamid, cymoxanil, fiuopicolide, or mandipropamid was not detected within any lineage. Thus, current U.S. populations of P. infestans remained sensitive to mefenoxam during the displacement of the US-22 lineage by US-23 over the past 5 years. C1 [Saville, Amanda; Ristaino, Jean Beagle] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Graham, Kim] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Gruenwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Washington, DC 20250 USA. [Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gruenwald, Niklaus J.] Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. [Myers, Kevin; Fry, William E.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Ristaino, JB (reprint author), N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. EM Jean_Ristaino@ncsu.edu OI Ristaino, Jean/0000-0002-9458-0514; Grunwald, Niklaus/0000-0003-1656-7602 FU USDA AFRI [2011-68004-30154] FX This project was funded by a USDA AFRI grant # 2011-68004-30154. We thank DuPont, FMC, Syngenta, and Valent for supplying the fungicides for this study; E. Lassiter, C. Pearce, M. Harris, and NITA interns S. Turner, M. Wyatt, and K. Bellingham for their laboratory assistance in conducting trials; and all the collaborators who provided samples to USAblight.org for further study in this work, including K. Everts, A. Gevens, B. Guino, D. Johnson, S. Johnson, W. Kirk, M. McGrath, P. Roberts, G. Secor, K. Seebold, and C. Smart. NR 51 TC 6 Z9 6 U1 3 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 659 EP 666 DI 10.1094/PDIS-05-14-0452-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800013 ER PT J AU Cespedes-Sanchez, MC Naegele, RP Kousik, CS Hausbeck, MK AF Cespedes-Sanchez, M. C. Naegele, R. P. Kousik, C. S. Hausbeck, M. K. TI Field Response of Cucurbit Hosts to Pseudoperonospora cubensis in Michigan SO PLANT DISEASE LA English DT Article ID DOWNY MILDEW; POPULATIONS; INHERITANCE; RESISTANCE; MUSKMELON; VIRULENCE AB Downy mildew, caused by Pseudoperonospora cubensis, is a severe foliar disease of many cucurbit crops worldwide. Forty-one cucurbit cultigens (commercial cultivars and plant introductions) from five genera (Cucumis, Citrullus, Cucurbita, Lagenaria, and Luffa) were assessed for susceptibility to P. cubensis in a research field exposed to natural inoculum in Michigan. Eight cultigens from a differential set for pathotype determination were included within the 41 cultigens to detect changes in dominant P. cubensis pathotypes present. No pathotype differences were found between 2010 and 2011. Cucumis melo cultigen MR-1 was less susceptible to Michigan P. cubensis populations than other C. melo cultigens. No symptoms or signs of infection were detected on cultigens of Cucurbita moschata and C. pepo. Disease onset was later in 2011 than 2010; greater than 90% disease severity in pickling cucumber 'Vlaspik' was observed in both years. This study confirmed that Cucumis is the most susceptible cucurbit genus, while Citrullus and Cucurbita cultigens were the least susceptible genera to populations of P. cubensis in Michigan. Area under the disease progress curve values indicated that disease progress was limited on all Citrullus cultigens compared with Cucumis cultigens, and pathogen sporulation was not detected under field conditions. Future studies should evaluate the ability of a reduced fungicide program to control downy mildew on less susceptible Cucumis melo 'Edisto 47', 'Primo', 'Athena', 'Strike', 'Ananas', 'Banana', and 'Tam-Dew'. Many of the melon cultivars evaluated were selected on the basis of reported resistance to downy mildew, yet they showed significant disease symptoms. It is important to evaluate candidate cultigens for resistance to local P. cubensis populations. C1 [Cespedes-Sanchez, M. C.; Naegele, R. P.; Hausbeck, M. K.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Kousik, C. S.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Hausbeck, MK (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. EM hausbec1@msu.edu FU Agricultural Research Fund, Pickle Packers International, Inc.; Project GREEEN (a cooperative effort by Michigan plant-based commodities and businesses with the Michigan State University [MSU] AgBioResearch) [GR07-077, GR09-092, GR10-025]; MSU AgBio Research; Pickle and Pepper Research Committee of MSU, Pickle Packers International, Inc. FX This research is based upon work supported by the Agricultural Research Fund, Pickle Packers International, Inc.; Project GREEEN (a cooperative effort by Michigan plant-based commodities and businesses with the Michigan State University [MSU] AgBioResearch) award numbers GR07-077, GR09-092, and GR10-025; MSU AgBio Research; and the Pickle and Pepper Research Committee of MSU, Pickle Packers International, Inc. We thank J. Fry for statistical assistance. NR 28 TC 2 Z9 2 U1 1 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 676 EP 682 DI 10.1094/PDIS-05-14-0500-RE PG 7 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800015 ER PT J AU Fan, X Zhang, J Yang, L Wu, MD Chen, WD Li, GQ AF Fan, Xuan Zhang, Jing Yang, Long Wu, Mingde Chen, Weidong Li, Guoqing TI Development of PCR-Based Assays for Detecting and Differentiating Three Species of Botrytis Infecting Broad Bean SO PLANT DISEASE LA English DT Article ID CINEREA PERS.; REAL-TIME; CHOCOLATE SPOT; QUANTIFICATION; IDENTIFICATION; STRAWBERRY; DISEASE; GRAPES; CHINA; FRUIT AB Botrytis cinerea, B. fabae, and B. fabiopsis are known to cause chocolate spot on broad bean. This study was conducted to develop polymerase chain reaction (PCR)-based assays to detect and differentiate these three species. Two sets of primers, Bc-f/Bc-r for B. cinerea and Bfab-f/Bfab-r for B. fabiopsis, were designed based on two sequence-characterized amplified region markers derived from two random amplified polymorphic DNA assays. The other primer set, Bfa-f/Bfa-r for B. fabae, was designed based on the necrosis and ethylene-inducing protein I gene sequence. The three primer sets were highly specific for the corresponding species of Botrytis in both single and multiplex PCR assays. The PCR detection limit was 40, 40, and 400 pg of DNA per 25-mu l reaction mixture for B. fabae, B. fabiopsis, and B. cinerea, respectively. Presence of the broad bean DNA in the PCR reactions at 1:1000 (Botrytis DNA/broad bean DNA [wt/wt]) had negligible effects on detection of the targeted Botrytis spp. The multiplex PCR assay was able to detect three Botrytis spp. in artificially infected and naturally infected broad bean leaves. These results suggest that the multiplex PCR assay developed in this study could be used to monitor the epidemics of chocolate spot of broad bean in the field. C1 [Fan, Xuan; Zhang, Jing; Yang, Long; Wu, Mingde; Li, Guoqing] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Fan, Xuan; Zhang, Jing; Yang, Long; Wu, Mingde; Li, Guoqing] Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China. [Chen, Weidong] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Zhang, J (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. EM zhangjing1007@mail.hzau.edu.cn FU R & D Special Fund for Public Welfare Industry (Agriculture) of China [201303025]; National Natural Science Foundation of China [31070122, 31301615] FX This research was funded by the R & D Special Fund for Public Welfare Industry (Agriculture) of China (grant number 201303025) and the National Natural Science Foundation of China (grant numbers 31070122 and 31301615). NR 31 TC 4 Z9 4 U1 6 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 691 EP 698 DI 10.1094/PDIS-07-14-0701-RE PG 8 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800017 ER PT J AU Li, YY Xiao, L Tan, GL Fu, XP Li, RH Li, F AF Li, Y. Y. Xiao, L. Tan, G. L. Fu, X. P. Li, R. H. Li, F. TI First Report of Tomato spotted wilt virus on Celery in China SO PLANT DISEASE LA English DT News Item C1 [Li, Y. Y.; Xiao, L.; Li, F.] Yunnan Agr Univ, China Educ Minist, Key Lab Agr Biodivers Pest Management, Kunming 650201, Peoples R China. [Tan, G. L.] Yunnan Agr Univ, Modem Educ Technol Ctr, Kunming 650201, Peoples R China. [Fu, X. P.] Yunnan Agr Univ, Coll Food Sci & Technol, Kunming 650201, Peoples R China. [Li, R. H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Li, YY (reprint author), Yunnan Agr Univ, China Educ Minist, Key Lab Agr Biodivers Pest Management, Kunming 650201, Peoples R China. NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2015 VL 99 IS 5 BP 734 EP 735 DI 10.1094/PDIS-11-14-1105-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CQ8NU UT WOS:000360865800050 ER PT J AU Zielinski, WJ Schlexer, FV AF Zielinski, William J. Schlexer, Fredrick V. TI Changes in the Structural and Functional Characteristics of Fisher (Pekania pennanti) Rest Structures Over Time SO NORTHWEST SCIENCE LA English DT Article DE fisher; Pekania pennanti; rest structures; snags; cavities ID SIERRA-NEVADA; HABITAT SELECTION; FOREST; DECAY; CALIFORNIA; SNAGS; BIODIVERSITY; LONGEVITY; SURVIVAL; DYNAMICS AB Resting habitat used by fishers (Pekania pennanti) has been relatively well studied but information on the persistence of their resting structures over time is unknown. We selected for reexamination 73 of 195 resting structures used by by fishers in northwestern California and compared their condition on the date they were found with their condition 8.2-12.1 years later. Resting structures were classified into three functional groups: live trees, snags, and logs. Each group was classified further into structural stages of decay or decomposition. A total of 19.2% of resting structures changed structural stage over the assessment period, transitioning from one structural stage to another within or between groups. However, none of the structures declined in function to the point that they could not be used for resting. One tree jumped two functional groups, transitioning from a live tree to a log. Based on the rates of degradation that we observed, we recommend monitoring the fate of fisher resting structures every few decades. This will help us provide information about the loss rate of resting structures that, when combined with new information about the rate of cavity creation in trees and snags, will help us determine whether the forces of resting structure loss and gain are in dynamic equilibrium. C1 [Zielinski, William J.; Schlexer, Fredrick V.] US Forest Serv, USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. RP Zielinski, WJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU USDA Forest Service, Pacific Southwest Research Station FX We thank A. Viducich for assisting with relocating rest structures and the USDA Forest Service, Pacific Southwest Research Station for support and funding. NR 32 TC 0 Z9 0 U1 5 U2 7 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD MAY PY 2015 VL 89 IS 2 BP 148 EP 153 PG 6 WC Ecology SC Environmental Sciences & Ecology GA CP8CR UT WOS:000360119400004 ER PT J AU Akbar, MA Lu, YQ Chen, MJ Elshikha, AS Ahamed, R Brantly, ML Chang, LJ Holliday, LS Cao, JJ Song, SH AF Akbar, Mohammad A. Lu, Yuanqing Chen, Mong-Jen Elshikha, Ahmed S. Ahamed, Rubina Brantly, Mark L. Chang, Lung-ji Holliday, Lexie S. Cao, Jay J. Song, Sihong TI Alpha-1 Antitrypsin (AAT) Gene and Stem Cell Based Therapies for the Treatment of Osteoporosis SO MOLECULAR THERAPY LA English DT Meeting Abstract CT 18th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT) CY MAY 13-16, 2015 CL New Orleans, LA SP Amer Soc Gene & Cell Therapy C1 [Akbar, Mohammad A.; Chen, Mong-Jen; Elshikha, Ahmed S.; Ahamed, Rubina; Song, Sihong] Univ Florida, Pharmaceut, Gainesville, FL USA. [Lu, Yuanqing; Brantly, Mark L.] Univ Florida, Med, Gainesville, FL USA. [Chang, Lung-ji] Univ Florida, Mol Genet & Microbiol, Gainesville, FL USA. [Holliday, Lexie S.] Univ Florida, Orthodont, Gainesville, FL USA. [Cao, Jay J.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1525-0016 EI 1525-0024 J9 MOL THER JI Mol. Ther. PD MAY PY 2015 VL 23 SU 1 MA 508 BP S203 EP S204 PG 2 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA CP5UY UT WOS:000359951700511 ER PT J AU Paul, DR Kramer, M Stote, KS Baer, DJ AF Paul, David R. Kramer, Matthew Stote, Kim S. Baer, David J. TI Determinants of Variance in the Habitual Physical Activity of Overweight Adults SO JOURNAL OF PHYSICAL ACTIVITY & HEALTH LA English DT Article DE activity monitor; sample size; accelerometry ID ACTIVITY MONITORS; ACCELEROMETER AB Background: The number of days of data and number of subjects necessary to estimate total physical activity (TPA) and moderate-to-vigorous physical activity (MVPA) requires an understanding of within- and between-subject variances, and the influence of sex, body composition, and age. Methods: Seventy-one adults wore accelerometers for 7-day intervals over 6 consecutive months. Results: Body fat and sex influenced TPA and MVPA. The sources of subject-related variation for TPA and MVPA were within-subject (48.4% and 54.3%), between-subject (34.3% and 31.8%), and calendar effects (17.3% and 13.9%). Based on within-subject variances, the error associated with estimating TPA and MVPA by collecting 1 to 7 days of data ranged from 28.2% to 13.3% for TPA and 62.0% to 28.6% for MVPA. Based on between-subject variances, detecting a 10% difference between 2 groups at a power of 90% requires approximately 200 and 725 subjects per group for TPA and MVPA, respectively. Conclusions: Estimates of MVPA are more variable than TPA in overweight adults, therefore more days of data are required to estimate MVPA and larger sample sizes to detect treatment differences for MVPA. Log-transforming data reduces the need for additional days of data collection, thereby improving chances of detecting treatment effects. C1 [Paul, David R.] Univ Idaho, Dept Movement Sci, Moscow, ID 83843 USA. [Kramer, Matthew] Biometr Consulting Serv, USDA, Beltsville, MD USA. [Stote, Kim S.; Baer, David J.] USDA, Food Components & Hlth Lab, Beltsville, MD 20705 USA. RP Paul, DR (reprint author), Univ Idaho, Dept Movement Sci, Moscow, ID 83843 USA. EM dpaul@uidaho.edu FU U.S. Dairy Export Council; United States Department of Agriculture FX This work was supported by the U.S. Dairy Export Council and the United States Department of Agriculture. NR 17 TC 0 Z9 0 U1 0 U2 1 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1543-3080 EI 1543-5474 J9 J PHYS ACT HEALTH JI J. Phys. Act. Health PD MAY PY 2015 VL 12 IS 5 BP 680 EP 685 DI 10.1123/jpah.2013-0269 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CO5DD UT WOS:000359178900013 PM 24834467 ER PT J AU South, EC Kondo, MC Cheney, RA Branas, CC AF South, Eugenia C. Kondo, Michelle C. Cheney, Rose A. Branas, Charles C. TI Neighborhood Blight, Stress, and Health: A Walking Trial of Urban Greening and Ambulatory Heart Rate SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID VIOLENT CRIME; DISORDER; VACANT; DISEASE; ENVIRONMENT; EXPOSURE; SAFETY; BRAIN; SPACE AB We measured dynamic stress responses using ambulatory heart rate monitoring as participants in Philadelphia, Pennsylvania walked past vacant lots before and after a greening remediation treatment of randomly selected lots. Being in view of a greened vacant lot decreased heart rate significantly more than did being in view of a nongreened vacant lot or not in view of any vacant lot. Remediating neighborhood blight may reduce stress and improve health. C1 [South, Eugenia C.] Univ Penn, Perelman Sch Med, Robert Wood Johnson Clin Scholars Program, Philadelphia, PA 19104 USA. [South, Eugenia C.] Perelman Sch Medicine, Dept Emergency Med, Philadelphia, PA USA. [Kondo, Michelle C.; Cheney, Rose A.; Branas, Charles C.] Perelman Sch Med, Urban Hlth Lab, Philadelphia, PA USA. [Kondo, Michelle C.] US Forest Serv, USDA, No Res Stn, Philadelphia, PA USA. [Branas, Charles C.] Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA USA. RP South, EC (reprint author), Dept Emergency Med, Ground Floor Ravdin,3400 Spruce St, Philadelphia, PA 19104 USA. EM eugenia.south@uphs.upenn.edu OI Kondo, Michelle/0000-0001-9152-1589 FU National Institutes of Health ([NIH]) [R01AA020331, R01AA016187]; Centers for Disease Control and Prevention ([CDC]) [U49CE001093]; Robert Wood Johnson Foundation (RWJF) Health and Society Education Fund; US Department of Agriculture (USDA), Forest Service FX This work was supported by the National Institutes of Health ([NIH] grants R01AA020331 and R01AA016187 to C. C. B.), the Centers for Disease Control and Prevention ([CDC] grant U49CE001093), and the Robert Wood Johnson Foundation (RWJF) Health and Society Education Fund (to E. C. S.), with additional funding from the US Department of Agriculture (USDA), Forest Service (to M. C. K.). NR 30 TC 9 Z9 9 U1 5 U2 14 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD MAY PY 2015 VL 105 IS 5 BP 909 EP 913 DI 10.2105/AJPH.2014.302526 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CN3AN UT WOS:000358295600033 PM 25790382 ER PT J AU Young, DJN Porensky, LM Wolf, KM Fick, SE Young, TP AF Young, Derek J. N. Porensky, Lauren M. Wolf, Kristina M. Fick, Stephen E. Young, Truman P. TI Burning reveals cryptic plant diversity and promotes coexistence in a California prairie restoration experiment SO ECOSPHERE LA English DT Article DE California; Central Valley; controlled burns; diversity; fire; fire-induced recruitment; grassland; Grindelia; intraspecific aggregation; restoration; seed bank; Trifolium ID SEED-GERMINATION; TEMPORAL PRIORITY; TALLGRASS PRAIRIE; FIRE SEASON; GRASSLAND; SMOKE; VEGETATION; COMMUNITY; HETEROGENEITY; COMPETITION AB Grassland and prairie restoration projects in California often result in long-term establishment of only a few native plant species, even when they begin with a diverse seed palette. One explanation for the disappearance of certain native species over time is that they are excluded through competition. If so, management that reduces interspecific competition may favor "subordinate'' natives and promote greater native species diversity in restored communities. Potential management approaches to accomplish this goal include intraspecific spatial aggregation during seeding and prescribed fire. However, no studies have experimentally evaluated the effects of fire on a controlled (restoration) species pool or the interaction between fire and spatial aggregation. In a previous California prairie restoration experiment, we demonstrated that aggregated plantings protected competitively subordinate species from exclusion and increased community diversity for three years. However, native species richness declined throughout the study, and the benefits of aggregated seeding had begun to disappear by the third year. For the present study, we resurveyed the experimental plots five years after seeding and in the following year carried out controlled burns on half of the plots. The four subordinate species and one of the previously dominant species continued to decline and essentially disappeared aboveground during years four and five. However, burning in year five decreased the cover of dominant natives, triggered the reappearance of the three subordinate species that had disappeared or nearly disappeared in previous years, and increased diversity of the restored community. Seeding treatments (aggregated or interspersed) did not significantly affect community-level responses to the burning treatment. These results confirm that although initial intraspecific aggregation may promote species coexistence in the short term, re-establishing disturbance regimes can allow coexistence over a longer time scale by revealing and potentially renewing seed bank diversity. C1 [Young, Derek J. N.; Wolf, Kristina M.; Fick, Stephen E.; Young, Truman P.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Young, Derek J. N.; Wolf, Kristina M.; Fick, Stephen E.; Young, Truman P.] Univ Calif Davis, Grad Grp Ecol, Davis, CA 95616 USA. [Porensky, Lauren M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. RP Young, DJN (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM djyoung@ucdavis.edu FU NSF [DEB 10-50543]; NSF REACH IGERT at the University of California, Davis [NSF DGE 0801430]; NSF Graduate Research Fellowships FX Jim Jackson and the agricultural field maintenance crew of UC Davis supported the field experiment in innumerable ways. John Anderson, Hedgerow Farms staff, Megan Lulow and Deborah Peterson made the study more relevant for local restoration by providing advice on species and seed rates. The UC Davis Fire Department crew, under Chief John J. Heilmann, executed the experimental burns. Funding for this study was provided by NSF DEB 10-50543, NSF REACH IGERT at the University of California, Davis (NSF DGE 0801430), and a NSF Graduate Research Fellowships to D. Young and L. Porensky. Authors' division of labor: L. Porensky designed and initiated the study, all authors performed fieldwork, D. Young analyzed the data, and all authors wrote the paper. NR 48 TC 1 Z9 1 U1 8 U2 28 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2015 VL 6 IS 5 AR 81 DI 10.1890/ES14-00303.1 PG 11 WC Ecology SC Environmental Sciences & Ecology GA CN4XE UT WOS:000358433000002 ER PT J AU Kelly, JD Trapp, J Miklas, PN Cichy, KA Wright, EM AF Kelly, James D. Trapp, Jennifer Miklas, Phillip N. Cichy, Karen A. Wright, Evan M. TI Registration of 'Desert Song' Flor de Junio and 'Gypsy Rose' Flor de Mayo Common Bean Cultivars SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Desert Song' Flor de Junio (FJ) common bean (Phaseolus vulgaris L.) (Reg. No. CV-308, PI 673424) and 'Gypsy Rose' Flor de Mayo (FM) common bean (Reg. No. CV-309, PI 673425) cultivars, developed by Michigan State University AgBioResearch, were released in 2014 as high-yielding FJ and FM seed types adapted to northern production zones. Both cultivars were developed using the pedigree breeding method to the F-4 generation followed by pure line selection for disease, agronomic, seed, and cooking traits. In 4 yr of field trials, Desert Song yielded 3468 kg ha(-1), flowered in 41 d, and matured in 100 d on average. Plants averaged 48 cm for canopy height, with lodging resistance score of 3.2 and seed weight of 36.2 g 100 seed(-1). In the same 4 yr of field trials, Gypsy Rose yielded 3761 kg ha(-1), flowered in 49 d, and matured in 105 d on average. Plants averaged 49 cm for canopy height, with lodging resistance score of 3.3 and seed weight of 30.4 g 100 seed(-1). Both cultivars combine high yield potential with adaptation to northern production areas. They possess resistance to Bean common mosaic virus and intermediate resistance to race 22: 2 of bean rust [incited by Uromyces appendiculatus (Pers.:Pers.) Unger]. Each cultivar produces seed that meets industry standards for packaging and canning in the respective FJ and FM seed classes. C1 [Kelly, James D.; Cichy, Karen A.; Wright, Evan M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Miklas, Phillip N.] USDA ARS, Forage & Vegetable Crop Res Unit, Prosser, WA 99350 USA. [Cichy, Karen A.] USDA ARS, E Lansing, MI 48824 USA. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu FU Michigan State University AgBioResearch; Michigan Crop Improvement Association; Michigan Bean Commission FX The authors wish to acknowledge Jorge Acosta, INIFAP, Celaya, Mexico, for providing original seed of FJ and FM lines used as parents; and for the financial support from the Michigan State University AgBioResearch, Michigan Crop Improvement Association, and the Michigan Bean Commission. NR 13 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 133 EP 137 DI 10.3198/jpr2014.05.0028crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900001 ER PT J AU Chamberlin, KD Bennett, RS Damicone, JP Godsey, CB Melouk, HA Keim, K AF Chamberlin, K. D. Bennett, R. S. Damicone, J. P. Godsey, C. B. Melouk, H. A. Keim, K. TI Registration of 'OLe' Peanut SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB OLe (Reg. No. CV-128, PI 674164) peanut (experimental designation ARSOK-S140-1OL) is a high-oleic spanish-type (Arachis hypogaea L. subsp. fastigiata var. vulgaris) that was cooperatively released by the USDA-ARS and the Oklahoma Agricultural Experiment Station in 2014. OLe is the product of the cross 'Tamspan 90' x F435. The male parent, F435, is the original donor of the high-oleic genes. Pedigree methodology was used in screening segregating populations. The advanced breeding line ARSOK-S140-1OL was identified due to its enhanced resistance to Sclerotinia blight (causal agent Sclerotinia minor Jagger) and pod rot (causal agents Pythium myriothylum and/or Rhizoctonia solani). In 6 yr of advanced breeding line performance trials across three locations in Oklahoma, OLe yielded an average of 4048 kg ha(-1) and had less incidence of Sclerotinia blight in 2009 to 2011 and 2013 and less pod rot in 2009 and 2010 (P < 0.05) compared with OLin. Overall, field performance data indicate that production of OLe instead of OLin could save producers $150 to $400 ha(-1) annually in disease management input costs. C1 [Chamberlin, K. D.; Bennett, R. S.] USDA ARS, Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Damicone, J. P.; Melouk, H. A.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [Godsey, C. B.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Keim, K.] Dow AgroSci LLC, Lubbock, TX 79452 USA. RP Chamberlin, KD (reprint author), USDA ARS, Peanut & Other Field Crops Res Unit, 1301 N Western Rd, Stillwater, OK 74075 USA. EM kelly.chamberlin@ars.usda.gov FU USDA-ARS; Oklahoma Peanut Commission; Oklahoma Agricultural Experiment Station FX OLe was developed with financial support from the USDA-ARS, the Oklahoma Peanut Commission, and the Oklahoma Agricultural Experiment Station. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 10 TC 1 Z9 1 U1 1 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 154 EP 158 DI 10.3198/jpr2014.10.0072crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900005 ER PT J AU Sandhu, HS Gilbert, RA Comstock, JC Gordon, VS Korndorfer, P El-Hout, N Arundale, RA AF Sandhu, Hardev S. Gilbert, Robert A. Comstock, Jack C. Gordon, Vanessa S. Korndoerfer, Pedro El-Hout, Nael Arundale, Rebecca A. TI Registration of 'UFCP 74-1010' Sugarcane SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID MICROSATELLITE MARKERS; BIOMASS AB Sugarcane 'UFCP 74-1010' (Reg. No. CV-160, PI 673048) was released by the University of Florida and the USDA-ARS, Canal Point, for its potential use in cellulosic ethanol production. UFCP 74-1010, a cross between sugarcane (a complex hybrid of Saccharum spp.) cultivars CP 66-56-4 and CL 47-83. UFCP 74-1010 is a high fiber sugarcane that has been released to improve biomass production for cellulosic ethanol production in Florida. UFCP 74-1010 is moderately resistant to smut (caused by Sporisorium scitamineum) with significantly lower infestation compared with a reference check, L 79-1002', under both natural environmental conditions and artificial inoculations. UFCP 74-1010 is also resistant to brown rust (caused by Puccinia melanocephala H. and P. Sydow), orange rust (caused by P. kuehnii), and mosaic (caused by Sugarcane mosaic virus) and is moderately resistant to leaf scald [Xanthomonas albilineans (Ashby) Dawson]. Mean dry biomass yield of UFCP 74-1010 (29.2 Mg ha(-1)) was approximately 5% higher than L 79-1002 (27.9 Mg ha(-1)), averaged across eight location-years of field trials. Plant composition of UFCP 74-1010 is similar to L 79-1002 with 41.9% cellulose, 27.8% hemicellulose, 22.4% lignin, 5.0% ash, and 1.6% structural protein. UFCP 74-1010 is released to be cultivated for cellulosic ethanol production on marginal or sandy soils of Florida for cellulosic ethanol production. C1 [Sandhu, Hardev S.] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Gilbert, Robert A.] Univ Florida, Gainesville, FL 32611 USA. [Comstock, Jack C.; Gordon, Vanessa S.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Korndoerfer, Pedro] Florida Crystals, South Bay, FL USA. [El-Hout, Nael] BP Biofuels North Amer LLC, Highlands Cty, FL USA. [Arundale, Rebecca A.] BP Biofuels North Amer, Houston, TX USA. RP Sandhu, HS (reprint author), Univ Florida, Everglades Res & Educ Ctr, 3200 E Palm Beach Rd, Belle Glade, FL 33430 USA. EM hsandhu@ufl.edu OI Gordon, Vanessa S/0000-0001-6490-6985 NR 27 TC 5 Z9 5 U1 2 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 179 EP 184 DI 10.3198/jpr2014.06.0042crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900010 ER PT J AU Zhang, GR Martin, TJ Fritz, AK Miller, R Chen, MS Bowden, RL Johnson, JJ AF Zhang, Guorong Martin, Terry J. Fritz, Allan K. Miller, Rebecca Chen, Ming-Shun Bowden, Robert L. Johnson, Jerry J. TI Registration of 'Oakley CL' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Oakley CL' (Reg. No. CV-1105, PI 670170) hard red winter wheat (Triticum aestivum L.) cultivar was developed at the Agricultural Research Center-Hays, Kansas State University, and released by the Kansas Agricultural Experiment Station in 2013. Oakley CL carries one Clearfield gene and has tolerance to Beyond herbicide (a.i. imazamox). Oakley CL was selected from a three-way cross of 'Above'/'Danby'//KS03HW10 using a modified bulk breeding method. The objective of the cross was to develop a hard winter wheat cultivar with herbicide resistance and adaptation to the semiarid climate in western Kansas and its adjacent regions. Oakley CL is an F 6 -derived line. Oakley CL was released primarily for its tolerance to Beyond herbicide, high grain yield potential under nonirrigated conditions in western Kansas, resistance to Wheat streak mosaic virus and stripe rust (caused by Puccinia striiformis Westend.), good end-use quality, and preharvest sprouting tolerance. C1 [Zhang, Guorong; Martin, Terry J.] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Fritz, Allan K.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Miller, Rebecca] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Bowden, Robert L.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Johnson, Jerry J.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. RP Zhang, GR (reprint author), Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. EM gzhang@ksu.edu FU Kansas Wheat Commission; BASF Corporation FX The contribution No. for this paper is 14-331-J from the Kansas Agricultural Experiment Station. Research was supported partially by the Kansas Wheat Commission and the BASF Corporation. The authors thank C. Seaman, A. Stegman, P. Geier, farmer cooperators, and colleagues at the KSU Research and Extension Stations and USDA-ARS for their help in conducting trials to evaluate Oakley CL. NR 14 TC 3 Z9 3 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 190 EP 195 DI 10.3198/jpr2014.04.0023crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900012 ER PT J AU Carter, AH Kidwell, KK DeMacon, V Balow, KA Shelton, GB Higginbotham, RW Chen, XM Engle, DA Baik, B Morris, CF AF Carter, A. H. Kidwell, K. K. DeMacon, V. Balow, K. A. Shelton, G. B. Higginbotham, R. W. Chen, X. M. Engle, D. A. Baik, B. Morris, C. F. TI Registration of 'Sprinter' Hard Red Winter Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PUCCINIA-STRIIFORMIS; NO-TILL; RESISTANCE; CULTIVARS; FALLOW AB High grain protein concentration and stripe rust (caused by Puccinia striiformis Westend. f. sp. tritici Eriks.) resistance are important traits for hard red winter wheat (Triticum aestivum L.) cultivars produced in the Pacific Northwest region of the United States. The objective of this research was to develop an adapted winter wheat cultivar that can consistently attain the desired level of grain protein concentration combined with effective stripe rust resistance. 'Sprinter' (Reg. No. CV-1106, PI 671896) hard red winter wheat was developed and released in August 2012 by the Agricultural Research Center of Washington State University. Sprinter was tested under the experimental designations KKHR05001, KKHR05-0-0-097, and WA008118, which were assigned through progressive generations of advancement. Sprinter is a standard height cultivar adapted to low to intermediate rainfall (<400 mm of average annual precipitation), unirrigated wheat production regions of Washington. Sprinter has high-temperature, adult-plant resistance to the stripe rust pathogen, high grain protein concentration, and high volume test weight, with excellent end-use quality properties similar or superior to those of 'Farnum', 'Finley', and 'Bauermeister'. C1 [Carter, A. H.; Kidwell, K. K.; DeMacon, V.; Balow, K. A.; Shelton, G. B.; Higginbotham, R. W.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Chen, X. M.; Engle, D. A.; Morris, C. F.] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Baik, B.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. RP Carter, AH (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM ahcarter@wsu.edu FU National Research Initiative Competitive Grants CAP from the USDA National Institute of Food and Agriculture [2011-68002-30029]; Washington State Grain Commission; Washington State Agriculture Research Center FX We greatly appreciate the dedicated assistance of the following support personnel who assisted with field testing, quality assessment, and disease screening during the development of this cultivar: Tracy Harris (WSU), Kent Evans (USDA-ARS), and Henry Wetzel (WSU). Molecular marker work was supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. We are grateful to the Washington State Grain Commission and the Washington State Agriculture Research Center for providing funding for this research. NR 12 TC 1 Z9 1 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 196 EP 200 DI 10.3198/jpr2014.05.0039crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900013 ER PT J AU Anderson, JA Wiersma, JJ Linkert, GL Reynolds, S Kolmer, JA Jin, Y Dill-Macky, R Hareland, GA AF Anderson, J. A. Wiersma, J. J. Linkert, G. L. Reynolds, S. Kolmer, J. A. Jin, Y. Dill-Macky, R. Hareland, G. A. TI Registration of 'Rollag' Spring Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID HEAD-BLIGHT RESISTANCE; F-SP TRITICI; MARKERS AB Fusarium head blight (FHB) (caused primarily by Fusarium graminearum Schwabe) is a disease that annually threatens wheat (Triticum aestivum L.) grown in the northern plains of the United States. Resistance to this disease is a high priority trait in the University of Minnesota's spring wheat breeding program. We utilized marker-assisted selection in the F-6 (pre-yield trial stage) generation to identify lines containing Fhb1, a major quantitative trait locus for FHB resistance. One line, tested as MN05214-3 in Minnesota statewide yield trials from 2008 to 2010 and in the Uniform Hard Red Spring Wheat Regional Nursery in 2009 and 2010, was released as 'Rollag' (Reg. No. CV-1107, PI 665250) by the University of Minnesota Agricultural Experiment Station in 2011. Rollag is a mid-maturity, semidwarf cultivar that has above-average grain protein content. Rollag was released on the basis of its good resistance to FHB, resistance to lodging, competitive grain yield and acceptable end-use quality characteristics. Rollag is well adapted to hard red spring wheat growing regions in Minnesota and the Upper Midwest. C1 [Anderson, J. A.; Wiersma, J. J.; Linkert, G. L.; Reynolds, S.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Kolmer, J. A.; Jin, Y.] Univ Minnesota, USDA ARS, St Paul, MN 55108 USA. [Dill-Macky, R.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Hareland, G. A.] N Dakota State Univ, USDA ARS, Fargo, ND 58105 USA. RP Anderson, JA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM ander319@umn.edu FU Minnesota Agricultural Experiment Station; Minnesota Wheat Research and Promotion Council; U.S. Department of Agriculture-Agricultural Research Service [59-0790-9025, 59-0206-9-070]; National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service; CAP [2006-55606-16629]; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX Rollag was developed with financial support from the Minnesota Agricultural Experiment Station, the Minnesota Wheat Research and Promotion Council, and the U.S. Department of Agriculture-Agricultural Research Service under Agreement Nos. 59-0790-9025 and 59-0206-9-070 and the National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP Grant No. 2006-55606-16629 and National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. NR 19 TC 0 Z9 1 U1 0 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 201 EP 207 DI 10.3198/jpr2014.07.0048crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900014 ER PT J AU Beaver, JS Rosas, JC Porch, TG Pastor-Corrales, MA Godoy-Lutz, G Prophete, EH AF Beaver, J. S. Rosas, J. C. Porch, T. G. Pastor-Corrales, M. A. Godoy-Lutz, G. Prophete, E. H. TI Registration of PR0806-80 and PR0806-81 White Bean Germplasm Lines with Resistance to BGYMV, BCMV, BCMNV, and Rust SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID POTYVIRUS RESISTANCE; I-GENE; COMMON; MARKER AB PR0806-80 (Reg. No. GP-296, PI 672994) and PR0806-81 (Reg. No. GP-297, PI 672995) are multiple disease-resistant dry bean (Phaseolus vulgaris L.) germplasm lines adapted to the humid tropics, developed and released cooperatively by the University of Puerto Rico Agricultural Experiment Station, the USDA-ARS, the Escuela Agricola Panamericana (Zamorano), the Instituto Dominicano de Investigaciones Agropecuarias y Forestales, and the Ministry of Agriculture, Natural Resources and Rural Development of the Republic of Haiti. The breeding objective was to develop white dry bean lines that combine resistance to Bean common mosaic virus (BCMV), Bean common mosaic necrotic virus (BCMNV), Bean golden yellow mosaic virus (BGYMV), and rust, caused by Uromyces appendiculatus (Pers.) Unger. PR0806-80 and PR0806-81 were developed by pedigree selection to the F-6 generation on the basis of superior agronomic traits, disease resistance, and commercial seed type. Advanced generation lines were screened for rust resistance in Honduras and were resistant to BGYMV, BCMNV, and rust in trials planted in Puerto Rico, the Dominican Republic, and Haiti. Results from greenhouse inoculations suggest that the lines combine the Ur-4, Ur-5, and Ur-11 alleles of rust resistance genes. Greenhouse inoculations were used to detect the presence of the bc-3 gene for resistance to BCMV and BCMNV, and marker-assisted selection was used to identify the presence of the bgm-1 allele and the SW12 quantitative trait locus for BGYMV resistance. The presence of SA14 and SI19 sequence characterized amplified region (SCAR) markers provided further evidence that these lines have the rust resistance genes Ur-4 and Ur-5. The lines were evaluated in field trials in the Dominican Republic, Haiti, and Puerto Rico. Mean seed yields of PR0806-80 (2048 kg ha(-1)) and PR0806-81 (2091 kg ha(-1)) were comparable to the check cultivar 'Verano' (2251 kg ha(-1)). These multiple disease-resistant white lines should be useful as parents to enhance the virus and rust resistance of white, small red, and black beans. C1 [Beaver, J. S.] Univ Puerto Rico, Dept Crop & Agroenvironm Sci, Mayaguez, PR 00681 USA. [Rosas, J. C.] Escuela Agr Panamer, Tegucigalpa, Honduras. [Porch, T. G.] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Pastor-Corrales, M. A.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20783 USA. [Godoy-Lutz, G.] Inst Dominicano Invest Agr & Forestales, Estn Expt Arroyo Loro, San Juan De La Maguna, Dominican Rep. [Prophete, E. H.] Minist Agr, Serv Natl Semencier, Port Au Prince, Haiti. RP Beaver, JS (reprint author), Univ Puerto Rico, Dept Crop & Agroenvironm Sci, Mayaguez, PR 00681 USA. EM james.beaver@upr.edu NR 16 TC 1 Z9 1 U1 0 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 208 EP 211 DI 10.3198/jpr2014.09.0061crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900015 ER PT J AU Isbell, TA Cermak, SC Dierig, DA Eller, FJ Marek, LF AF Isbell, Terry A. Cermak, Steven C. Dierig, David A. Eller, Fred J. Marek, Laura F. TI Registration of Katelyn Thlaspi arvense L. (Pennycress) with Improved Nondormant Traits SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID CANADIAN WEEDS; GERMINATION; BIOLOGY AB Katelyn (Reg. No. GP-35, PI 673443) pennycress (Thlaspi arvense L.) germplasm was publicly released by the USDA-ARS in 2014 as part of a new crop improvement program. Katelyn was developed by two generations of mass selection based on the germination response of freshly harvested pennycress seeds. The original seed source was the wild population Beecher (PI 672505) collected from a winter fallow cornfield 3.2 km north of Hanna City, IL. The germination rate of the original parent seed under conditions of 12-h light/dark cycles at 27.5 degrees C/11.5 degrees C was 0%. Beecher seed kept in the dark under otherwise identical conditions germinated at 7% compared with Katelyn S-2 seed, which had an immediate post-harvest germination rate of 91% for seed kept in the dark and 81% under the 12-h light/dark 27.5 degrees C/11.5 degrees C conditions. C1 [Isbell, Terry A.; Cermak, Steven C.; Eller, Fred J.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Dierig, David A.] USDA ARS, Natl Ctr Genet Resource Preservat, Ft Collins, CO 80521 USA. [Marek, Laura F.] Iowa State Univ Sci & Technol, USDA ARS, North Cent Plant Intro Stn, Dept Agron, Ames, IA 50011 USA. [Marek, Laura F.] Iowa State Univ, Ames, IA 50011 USA. RP Isbell, TA (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM terry.isbell@ars.usda.gov NR 10 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 212 EP 215 DI 10.3198/jpr2014.08.0053crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900016 ER PT J AU Carter, TE Todd, SM Gillen, AM AF Carter, T. E., Jr. Todd, S. M. Gillen, A. M. TI Registration of N6002 Soybean Germplasm with Enhanced Yield Derived from Japanese Cultivars Fukuyutaka and Nakasennari and Elevated Seed Protein Content SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID RESISTANCE; SELECTION; PEDIGREE; GENE AB The release of N6002 (Reg. No. GP-397, PI 674171) soybean [Glycine max L. (Merr.)] is part of an effort to broaden the genetic base of North American soybean breeding programs. N6002 was developed and released by the USDA-ARS and the North Carolina Agricultural Research Service in September 2014 as a conventional maturity group (MG) VI soybean germplasm derived from hybridization of the cultivar Young and USDA germplasm line N6202. N6002 is an F-4 - derived line that traces 25% of its pedigree to the Japanese cultivars Fukuyutaka and Nakasennari. The remaining pedigree (75%) is derived from 'Young'. N6002 was tested in three sets of multistate yield trials in the southern United States: recombinant inbred line (RIL) yield trials, the United Soybean Board Southern Diversity Yield Trial Project, and the USDA Uniform Soybean Tests-Southern States. Over 39 environments, N6002 averaged 8.3% higher yield than parents Young or N6202, and 97% of the elite check 'NC-Roy'. Although seed yield of N6002 was higher than Young, its seed protein content was comparable to that of Young and greater than that of NC-Roy. The superior yield of N6002 compared with Young demonstrates that yield-enhancing alleles were transferred to the Young background from Japanese cultivars. The elevated seed protein content of N6002 compared with NC-Roy and the parity of protein content with Young suggest that these yield-enhancing alleles may aid in mitigating the well-known negative correlation between seed yield and protein content. C1 [Carter, T. E., Jr.; Todd, S. M.] USDA ARS, Raleigh, NC 27607 USA. [Gillen, A. M.] USDA ARS, Stoneville, MS 38776 USA. RP Carter, TE (reprint author), USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. EM Thomas.Carter@ars.usda.gov FU United Soybean Board FX The authors would like to thank the collaborators in the United Soybean Board-sponsored Diversity Trial Project, USDA Uniform Tests, and RIL yield trials for managing yield trials and sharing data. The authors would like to thank the United Soybean Board for funding. NR 28 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 216 EP 221 DI 10.3198/jpr2014.09.0058crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900017 ER PT J AU Abdel-Haleem, H Boerma, HR Carter, TE Wood, ED Li, ZL AF Abdel-Haleem, Hussein Boerma, H. Roger Carter, Thomas E., Jr. Wood, E. Dale Li, Zenglu TI Registration of G07-6012 and G07-6029 Soybean Germplasm, Which Derive 50% Pedigree from Wild Soybean SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GLYCINE-SOJA; YIELD; SEED; CULTIVARS; ALLELES; PROTEIN; QTL AB Two soybean [Glycine max (L.) Merr.] germplasm lines, G0-76012 (Reg. No. GP-394, PI 672992) and G07-6029 (Reg. No. GP-395, PI 672993), were developed and released by the University of Georgia Agricultural Experiment Stations. Both G07-6012 and G07-6029 are F-4-derived lines from the hybridization of 'N7103' x PI 366122, a wild soybean (G. soja Siebold & Zucc.) accession from Japan. G07-6029 is an early maturity group VII line, which yielded 2253 kg ha(-1), or 88% of the yield of its elite parent N7103, and 78 and 84% of the yield from cultivars NC-Roy and AGS 758 RR, respectively, over 15 replicated trials in the southern United States. G07-6012 is a late maturity group VII line, which yielded 88% as much as N7103 and 70, 76, 80, and 82% as the check cultivars 'N7002', 'Woodruff', 'NC-Raleigh', and 'N8001', respectively, in these same trials. Both G07-6029 and G07-6012 have seed quality and shattering and lodging resistance scores similar to those of the check cultivars. Both G07-6012 and G07-6029 have smaller seed than their G. max parent, N7103 (6.5 g 100-seed weight for G07-6029 and 5.0 g 100-seed weight for G07-6012 compared with 8.0 g 100-seed weight for N7013). These two germplasm releases are the first in the southern United States that were derived from a single cross involving wild soybean. Because of their diverse pedigree (50% from G. soja) and positive agronomic performance, G07-6012 and G07-6029 are released as valuable genetic resources for applied soybean breeding programs. C1 [Abdel-Haleem, Hussein; Boerma, H. Roger; Wood, E. Dale; Li, Zenglu] Univ Georgia, Inst Plant Breeding Genet & Genom, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Abdel-Haleem, Hussein; Boerma, H. Roger; Wood, E. Dale; Li, Zenglu] Univ Georgia, Dept Crop & Soil Sci, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Boerma, H. Roger] Georgia Seed Dev Commiss, Athens, GA 30605 USA. [Carter, Thomas E., Jr.] USDA ARS, Soybean & Nitrogen Fixat Unit, Raleigh, NC 27607 USA. RP Li, ZL (reprint author), Univ Georgia, Inst Plant Breeding Genet & Genom, Ctr Appl Genet Technol, 111 Riverbend Rd, Athens, GA 30602 USA. EM zli@uga.edu FU United Soybean Board FX This research was supported by funds allocated to the Georgia Agricultural Experiment Stations and by the United Soybean Board. Appreciation is extended to Dr. Pengyin Chen (University of Arkansas, Fayetteville, AR), Dr. Grover Shannon (University of Missouri, Portageville, MO), and Dr. Katy Martin Rainy (Virginia Tech, Blacksburg, VA) for their support in data collection. We would also like to thank Steve Finnerty (University of Georgia, Athens, GA) for conducting the southern root-knot nematode evaluation. NR 31 TC 0 Z9 0 U1 3 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 222 EP 226 DI 10.3198/jpr2014.09.0059crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900018 ER PT J AU McGrath, JM Hanson, LE Panella, L AF McGrath, J. Mitchell Hanson, Linda E. Panella, Lee TI Registration of SR98 Sugarbeet Germplasm with Resistances to Rhizoctonia Seedling and Crown and Root Rot Diseases SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID SMOOTH-ROOT; DAMPING-OFF; SUCROSE; SOLANI; EL51 AB Sugarbeet (Beta vulgaris L.) germplasm SR98 (Reg. No. GP-287; PI 655951) was developed and released by the USDA-ARS at East Lansing, MI, in cooperation with the Beet Sugar Development Foundation, Denver, CO, and Michigan State University AgBioResearch, East Lansing, MI, to provide improved resistance to seedling disease and crown and root rot caused by Rhizoctonia solani Kuhn in a smooth-root genetic background that contributes to low soil tare. Previous smooth-root releases have been highly susceptible to diseases caused by R. solani. SR98 was derived from Rhizoctonia-resistant germplasm released from the USDA-ARS sugarbeet germplasm enhancement programs at Ft. Collins, CO, and East Lansing, MI, and has shown good yield potential in agronomic trials and moderate resistance to Aphanomyces blackleg (caused by Aphanomyces cochliodes Drechs.) and Cercospora leaf spot (caused by Cercospora beticola, Sacc.). SR98 can be used as a pollinator for hybrid production or a population from which breeders can select pollinators for developing Rhizoctonia-resistant hybrids adapted to the Great Lakes growing region. C1 [McGrath, J. Mitchell; Hanson, Linda E.] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Panella, Lee] USDA ARS, Crops Res Lab, Ft Collins, CO 80526 USA. RP McGrath, JM (reprint author), Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, 494 PSSB, E Lansing, MI 48824 USA. EM mitchmcg@msu.edu FU Michigan State University AgBioResearch FX Contributions of the Beet Sugar Development Foundation and the support of its member companies to the USDA-ARS East Lansing, MI, program, as well as support of the Michigan State University AgBioResearch (formerly Michigan Agricultural Experiment Station), is gratefully acknowledged. Thanks are also extended to Timothy M. Duckert for his expert technical assistance. Efforts of Dr. Robert Lewellen of the USDA-ARS, J. Miller and M. Rekoske of Betaseed, Inc., and S. Shaw, T. Goodwill, and R. Naegele at East Lansing in providing valuable disease nursery and agronomic testing assistance is gratefully acknowledged. NR 23 TC 0 Z9 0 U1 2 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 227 EP 231 DI 10.3198/jpr2013.08.0052crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900019 ER PT J AU Hulke, BS Gulya, TJ AF Hulke, Brent S. Gulya, Thomas J. TI Registration of the Oilseed Restorer Sunflower Germplasms RHA 472, RHA 473, RHA 474, and RHA 475, Possessing Resistance to Sclerotinia Head Rot SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID OLEIC-ACID CONTENT; IMIDAZOLINONE; DESATURASE; TRAIT; LOCI AB The most significant disease threat to sunflower (Helianthus annuus L.) production in most of the world is Sclerotinia head rot (HR), caused by the necrotrophic pathogen Sclerotinia sclerotiorum (Lib.) de Bary. Resistance to Sclerotinia is available in some inbred lines, but no line or hybrid is immune to it and all resistance is quantitatively inherited. There is also a lack of sunflower germplasms with high yield potential, good agronomic performance, and seed quality traits with Sclerotinia resistance. Herein, we describe the development and release of four germplasm lines that each has Sclerotinia HR resistance, competitive yield and oil content, good agronomic performance, and adaptation to the northern Great Plains of the United States and similar regions. RHA 472 (Reg. No. GP-329, PI 670490), RHA 473 (Reg. No. GP-330, PI 670491), RHA 474 (Reg. No. GP-331, PI 670492), and RHA 475 (Reg. No. GP-332, PI 670493) are restorer inbred germplasms that were developed using the pedigree breeding method with early generation testing for herbicide tolerance and fatty acid composition in the seed oil, and testcross hybrid evaluation in later generations for Sclerotinia resistance, yield, oil content, and agronomic performance. RHA 473 has high oleic acid in the seed oil, and RHA 474 and RHA 475 possess tolerance to imidazolinone herbicides. These lines were released by the USDA-ARS and the North Dakota Agricultural Experiment Station, Fargo, ND, to fill the urgent need in the sunflower industry for breeding lines with disease resistance in an elite genetic background. C1 [Hulke, Brent S.; Gulya, Thomas J.] USDA ARS, Sunflower & Plant Biol Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. RP Hulke, BS (reprint author), USDA ARS, Sunflower & Plant Biol Res Unit, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM brent.hulke@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 232 EP 238 DI 10.3198/jpr2014.12.0084crg PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900020 ER PT J AU Perumal, R Tesso, T Kofoid, KD Prasad, PVV Aiken, RM Bean, SR Wilson, JD Herald, TJ Little, CR AF Perumal, R. Tesso, T. Kofoid, K. D. Prasad, P. V. V. Aiken, R. M. Bean, S. R. Wilson, J. D. Herald, T. J. Little, C. R. TI Registration of Nine Grain Sorghum Seed Parent (A/B) Lines SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID DROUGHT TOLERANCE AB Nine sorghum [Sorghum bicolor (L.) Moench] A1 cytoplasmic male sterile seed parent (A) and their maintainer (B) lines (KS133A [Reg. No. PL-289, PI 674016], KS133B [Reg. No. PL-290, PI 674017], KS134A [Reg. No. PL-291, PI 674018], KS134B [Reg. No. PL-292, PI 674019], KS135A [Reg. No. PL-293, PI 674020], KS135B [Reg. No. PL-294, PI 674021], KS136A [Reg. No. PL-295, PI 674022], KS136B [Reg. No. PL-296, PI 674023], KS137A [Reg. No. PL-297, PI 674024], KS137B [Reg. No. PL-298, PI 674025], KS138A [Reg. No. PL-299, PI 674026], KS138B [Reg. No. PL-300, PI 674027], KS139A [Reg. No. PL-301, PI 674028], KS139B [Reg. No. PL-302, PI 674029], KS140A [Reg. No. PL-303, PI 674030], KS140B [Reg. No. PL-304, PI 674031], KS141A [Reg. No. PL-305, PI 674032], and KS141B [Reg. No. PL-306, PI 674033]) were released by the Kansas State University, Agricultural Research Center, Hays, KS, in August 2014. These nine lines were developed from random mating between the selected germplasm (PI550610B, IS2692R, IS9454R, IS9335R) and different genetic male-sterile (GMS) ms3 populations including KP8B, B51-B (plant, purple; seed white; glume, black), ms3 tan, and white tan B06-41701313 with different combinations using a recurrent selection followed by pedigree and backcross breeding methods. The lines KS133A/B to KS137A/B are early in maturity and come to flowering in 60 to 65 d, whereas the lines KS138A/B to KS141A/B are medium in maturity and come to flowering in 65 to 70 d. In general, these A/B lines are three-dwarf (dw1, Dw2, dw3, dw4) in height, are photoperiod-insensitive, and possess unique combinations of plant and seed color. All nine seed parent lines are tannin free, short in peduncle length, have panicles with good panicle exsertion, and have combining ability for yield and standability. C1 [Perumal, R.; Kofoid, K. D.] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Tesso, T.; Prasad, P. V. V.] Kansas State Univ, Throckmorton Plant Sci Ctr, Dept Agron, Manhattan, KS 66506 USA. [Aiken, R. M.] Kansas State Univ, Northwest Res Extens Ctr, Colby, KS 67701 USA. [Wilson, J. D.; Herald, T. J.] USDA ARS, CGAHR, Manhattan, KS 66502 USA. [Little, C. R.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Perumal, R (reprint author), Kansas State Univ, Agr Res Ctr Hays, 1232 240th Ave, Hays, KS 67601 USA. EM perumal@ksu.edu OI Bean, Scott/0000-0001-8678-8094 FU Kansas Grain Sorghum Commission; United Sorghum Checkoff Program; Great Plains Sorghum Improvement and Utilization Center FX We thank the Kansas Grain Sorghum Commission, United Sorghum Checkoff Program, and Great Plains Sorghum Improvement and Utilization Center for continuous funding support. The authors gratefully acknowledge Wayne Aschwege, Kansas State University, Agriculture Research Center, Hays, Kansas, for his assistance in planting and measurements. The authors also thank Chad Brady, Department of Plant Pathology, Kansas State University, Manhattan, KS, for his assistance in Fusarium stalk rot and charcoal rot screening. This paper is Contribution No. 15-116-J from the Kansas Agricultural Experiment Station, Manhattan. NR 9 TC 0 Z9 0 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 244 EP 248 DI 10.3198/jpr2014.09.0068crp PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900022 ER PT J AU Yerka, MK Toy, JJ Funnell-Harris, DL Sattler, SE Pedersen, JF AF Yerka, M. K. Toy, J. J. Funnell-Harris, D. L. Sattler, S. E. Pedersen, J. F. TI Registration of N619 to N640 Grain Sorghum Lines with Waxy or Wild-Type Endosperm SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID ETHANOL-PRODUCTION; STARCH; AMYLOSE AB Sorghum [Sorghum bicolor (L.) Moench] lines N619 to N636 (A lines; Reg. No. GS-699 to GS-716, PI 670134 to PI 670151); N619 to N636 (B lines; Reg. No. GS-721 to GS-738, PI 671777 to PI 671794); and N637 to N640 (R lines; Reg. No. GS-717 to GS-720, PI 670152 to PI 670155) comprise nine pairs of seed parent (A/B) lines, and two pairs of pollinator (R) lines (11 pairs total) that are near-isogenic for waxy (low-amylose) or wildtype endosperm. Breeding work was conducted jointly by the USDA-ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and the lines were released in May 2014. Release of these lines makes available two different waxy (wx) alleles (wx(a) and wx(b)) for development of grain sorghum as a source of low-amylose starch, whose end use is targeted to the ethanol and food industries. In particular, the release of wx and wild-type near-isogenic pairs facilitates the evaluation of agronomic performance of wx genotypes, and the release of both A/B and R lines facilitates the production of waxy grain hybrids. C1 [Yerka, M. K.; Toy, J. J.; Funnell-Harris, D. L.; Sattler, S. E.; Pedersen, J. F.] Univ Nebraska Lincoln, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Yerka, MK (reprint author), Univ Nebraska Lincoln, USDA ARS, Grain Forage & Bioenergy Res Unit, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM melinda.yerka@ars.usda.gov NR 17 TC 2 Z9 2 U1 2 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 249 EP 253 DI 10.3198/jpr2014.06.0043crgs PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900023 ER PT J AU Xin, ZG Gitz, D Burow, G Hayes, C Burke, JJ AF Xin, Zhanguo Gitz, Dennis Burow, Gloria Hayes, Chad Burke, J. J. TI Registration of Two Allelic Erect Leaf Mutants of Sorghum SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GRAIN-YIELD; ARCHITECTURE; MAIZE; POPULATION; ANGLE; RICE; PHOTOSYNTHESIS; GENE AB Two allelic sorghum [Sorghum bicolor (L.) Moench] erect leaf (erl) mutants were isolated from an Annotated Individually-pedigreed Mutagenized Sorghum (AIMS) mutant library developed at the Plant Stress and Germplasm Development Unit at Lubbock, TX. The two mutants, erl1-1 (Reg. No. GS-739, PI 673426) and erl1-2 (Reg. No. GS-740, PI 673427), were isolated from two independently pedigreed M-3 families mutagenized with ethyl methanesulfonate in a prominent sorghum inbred line, BTx623, which was used to sequence the sorghum genome. Both mutants were backcrossed three times to the wild-type (WT) BTx623 and shown to be a stable recessive mutation on a nuclear gene. The F-1 plants of a cross between the two mutants displayed the erect leaf phenotype, indicating the mutations that result in the erect leaf phenotype in the two mutants are allelic. Both mutants have upright and slightly short leaves and reduced height. The panicles from both mutants are smaller than WT even after three backcrosses. As a highly penetrant trait mediated by a single gene, these mutants may serve as a novel resource to design canopy architecture for optimizing radiation capture and use efficiency to increase biomass and grain yield. C1 [Xin, Zhanguo; Gitz, Dennis; Burow, Gloria; Hayes, Chad; Burke, J. J.] USDA ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Xin, ZG (reprint author), USDA ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA. EM zhanguo.xin@ars.usda.gov FU United Sorghum Checkoff Program; ARS CRIS [6208-21000-017-00D] FX The authors thank Lan Liu-Gitz, Rachel Smith, and Halee Hughes for excellent technical support. This work is supported by the United Sorghum Checkoff Program and the ARS CRIS project 6208-21000-017-00D. NR 23 TC 2 Z9 2 U1 1 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 254 EP 257 DI 10.3198/jpr2014.09.0060crgs PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900024 ER PT J AU Yerka, MK Toy, JJ Funnell-Harris, DL Sattler, SE Pedersen, JF AF Yerka, M. K. Toy, J. J. Funnell-Harris, D. L. Sattler, S. E. Pedersen, J. F. TI Registration of A/BN641 and RN642 waxy Grain Sorghum Genetic Stocks SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID ETHANOL-PRODUCTION; AMYLOSE CONTENT; STARCH; DIGESTIBILITY; BICOLOR; YIELD AB Loss-of-function mutations in the granule-bound starch synthase gene result in an endosperm with a waxy appearance and a near absence of starch amylose. Three waxy grain sorghum [Sorghum bicolor (L.) Moench] lines, AN641 (Reg. No. GS-741, PI 672150), BN641 (Reg. No. GS-742, PI 672151), and RN642 (Reg. No. GS-743, PI 672152) were developed jointly by the USDA-ARS and the Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska, and were released in June 2014. AN641 and BN641 have the waxy(b) (wx(b)) allele and are near-isogenic to 'Wheatland'. RN642 has the waxy(a) (wx(a)) allele and is near-isogenic to 'Tx430'. Release of these lines with cytoplasmic male-sterile (A), maintainer (B), and fertility restorer (R) fertility reactions to A 1 cytoplasm facilitates the production and evaluation of interallelic (wx(b) x wx(a)) waxy and heterowaxy [wx(b) x wild-type (WT) and WT x wx(a)] hybrids as a source of low-amylose starch for the ethanol and food industries. C1 [Yerka, M. K.; Toy, J. J.; Funnell-Harris, D. L.; Sattler, S. E.; Pedersen, J. F.] Univ Nebraska Lincoln, USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Yerka, MK (reprint author), Univ Nebraska Lincoln, USDA ARS, Grain Forage & Bioenergy Res Unit, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM melinda.yerka@ars.usda.gov NR 21 TC 1 Z9 1 U1 2 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 258 EP 261 DI 10.3198/jpr2014.10.0075crgs PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900025 ER PT J AU Pratt, RC Holland, JB Balint-Kurti, PJ Coles, ND Zwonitzer, JC Casey, MA McMullen, MD AF Pratt, R. C. Holland, J. B. Balint-Kurti, P. J. Coles, N. D. Zwonitzer, J. C. Casey, M. A. McMullen, M. D. TI Registration of the Ki14 x B73 Recombinant Inbred Mapping Population of Maize SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID QUANTITATIVE TRAIT LOCI; COMBINING ABILITY; GERMPLASM; RESISTANCE; LINES; CROSSES AB The Ohio Agricultural Research and Development Center released Ki14 x B73 maize (Zea mays L.) mapping population (Reg. No. MP-2, MGS 9025066 MAP; Maize Genetics COOP Stock Center no. Z042), a set of 119 recombinant inbred lines (RILs), in 2007. The mapping population was derived from a biparental cross between tropical inbred Ki14 (NCRPIS accession Ames 27259) and temperate inbred B73 (Reg. No. PL-17, PI 550473). One hundred sixteen of the original RILs were used for mapping quantitative trait loci associated with host resistance to foliar pathogens inciting southern corn leaf blight [caused by Cochliobolus heterostrophus (Drechs.)], gray leaf spot, (caused by Cercospora zeae-maydis Tehon & E.Y. Daniels), and northern corn leaf blight [caused by Setosphaeria turcica (Luttrell) K.J. Leonard & E.G. Suggs], three traits associated with maturity-days to anthesis, days to silking, and anther silk interval-and two morphological traits, plant and ear height. The genetic marker data included 765 single nucleotide polymorphisms and 74 simple sequence repeat markers genotyped on all the RILs and constructed into a genetic map. It is envisioned that the high level of host resistance of Ki14 and the agronomic performance of B73 will invite use of the population as a germplasm source for improved host resistance of temperate zone, and increased yield potential, of tropical zone maize. Distribution of the RIL mapping population will allow public access to this resource for continued mapping, gene discovery, and plant breeding. C1 [Pratt, R. C.; Casey, M. A.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Holland, J. B.; Coles, N. D.] N Carolina State Univ, USDA, ARS, Raleigh, NC 27695 USA. [Holland, J. B.; Coles, N. D.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Balint-Kurti, P. J.; Zwonitzer, J. C.] N Carolina State Univ, USDA, ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Balint-Kurti, P. J.; Zwonitzer, J. C.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [McMullen, M. D.] Univ Missouri, USDA, ARS, Plant Genet Res, Columbia, MO 65211 USA. RP Pratt, RC (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM ricpratt@nmsu.edu NR 24 TC 0 Z9 0 U1 3 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 262 EP 265 DI 10.3198/jpr2014.06.0041crmp PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900026 ER PT J AU Hwang, S King, CA Davies, MK Charlson, DV Ray, JD Cregan, PB Sneller, CH Chen, PY Carter, TE Purcell, LC AF Hwang, Sadal King, C. Andy Davies, Marilynn K. Charlson, Dirk V. Ray, Jeffery D. Cregan, Perry B. Sneller, Clay H. Chen, Pengyin Carter, Thomas E., Jr. Purcell, Larry C. TI Registration of the KS4895 x Jackson Soybean Mapping Population, AR93705 SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GLYCINE-MAX L.; QUANTITATIVE TRAIT LOCI; GENETIC-LINKAGE MAP; NITROGEN-FIXATION; GENOTYPIC DIFFERENCES; DROUGHT TOLERANCE; WATER DEFICITS; SEED PROTEIN; QTL ANALYSIS; YIELD AB AR93705 (Reg. No. MP-4, NSL 503796 MAP) is a soybean [Glycine max (L.) Merr.] mapping population developed by the University of Arkansas Experiment Station. The population consists of 15 F-3- and 76 F-5-derived recombinant inbred lines (RILs) from a cross between 'KS4895' (PI 595081) and 'Jackson' (PI 548657). The parents were originally chosen due to differences in sensitivity of N-2 fixation to drought, with Jackson being tolerant and KS4895 being sensitive. The population was selected to have a similar maturity, with a relative maturity group rating from approximately 5.2 to 5.7. The population was genotyped with 171 polymorphic simple sequence repeats by the USDA-ARS Crop Genetics Research Unit at Stoneville, MS, and with 493 polymorphic single nucleotide polymorphisms by the USDA-ARS Soybean Genomic and Improvement Laboratory in Beltsville, MD. Phenotypic data for the population were collected at multiple field sites for yield, canopy wilting, shoot ureide and N concentrations, stem N concentrations, nodule weight (mg plant(-1)), individual nodule weight (mg nodule(-1)), nodule size (mm), and nodule number per plant. Phenotypic and molecular-marker data were used to identify quantitative trait loci associated with these traits. The population offers a unique educational tool for molecular mapping exercises and genetics and for comparative physiology of drought-related, phenotypic extremes from the same genetic background. C1 [Hwang, Sadal; King, C. Andy; Davies, Marilynn K.; Chen, Pengyin; Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Charlson, Dirk V.] DuPont Pioneer, Soybean Prod Dev, Johnston, IA 50131 USA. [Ray, Jeffery D.] ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Cregan, Perry B.] ARS, USDA, Soybean Genom & Improvement Lab, BARC West, Beltsville, MD 20705 USA. [Sneller, Clay H.] Ohio State Univ, Dept Hort & Crop Sci OARDC, Wooster, OH 44691 USA. [Carter, Thomas E., Jr.] N Carolina State Univ, USDA, ARS, Dept Crop Sci, Raleigh, NC 27695 USA. RP Purcell, LC (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. EM lpurcell@uark.edu FU United Soybean Board; USDA-ARS [6420-21220-012-00D] FX The authors gratefully acknowledge partial financial support for this research from the United Soybean Board and from the USDA-ARS project number 6420-21220-012-00D. NR 40 TC 0 Z9 0 U1 0 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2015 VL 9 IS 2 BP 266 EP 271 DI 10.3198/jpr2014.05.0034crmp PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CN7HB UT WOS:000358603900027 ER PT J AU Whitelock, DP Armijo, CB Hughs, SE AF Whitelock, D. P. Armijo, C. B. Hughs, S. E. TI EVALUATING A PNEUMATIC FRACTIONATOR FOR CLEANING GINNED LINT SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Cotton ginning; Foreign matter; Lint cleaning; Pneumatic fractionator; Short fiber content AB The pneumatic fractionator has long been used to determine foreign matter content of seed cotton at the USDA-ARS cotton ginning laboratories. An experiment was conducted to evaluate the pneumatic fractionator as a device to remove foreign matter from ginned lint. No modifications were made to the standard device, except that air pressure to internal jets was reduced to 276 kPa (40 psi) from 517 kPa (70 psi). Three lint cleaning treatments after normal saw ginning were used: no lint cleaning, one standard controlled-batt saw lint cleaner, and cleaning with the pneumatic fractionator. Process times in the pneumatic fractionator were varied from 5 to 30 s. Foreign matter content (2.0 leaf grade and 253 counts g(-1) AFIS total foreign matter) and color measurements (78.69 Rd and 10.20 +b.) for the pneumatic fractionator were not statistically different from the saw lint cleaner (2.0 leaf grade, 257 counts g(-1) AFIS total foreign matter, 79.08 Rd, and 10.18 +b). Length measurements of pneumatic fractionator cleaned lint were similar to or better than those of lint with no lint cleaning and, in most cases, better than lint cleaned with a saw lint cleaner. For example, upper- half mean length for pneumatic fractionator cleaned, no lint cleaning, and saw lint cleaner fiber was 29.7, 30.0, and 29.2 mm (1.17, 1.18, and 1.15 in), respectively. Also, the pneumatic fractionator produced about the same number of fiber entanglements as the saw lint cleaner. Processing time in the pneumatic fractionator had very little effect on fiber quality parameters with the exception that the 5 s processing time had the highest levels of foreign matter. These results showed that the pneumatic fractionator cleaned lint about as well as a saw lint cleaner, but maintained fiber length better. C1 [Whitelock, D. P.; Armijo, C. B.; Hughs, S. E.] USDA ARS, Southwestern Cotton Ginning Res Lab, Mesilla Pk, NM 88047 USA. RP Whitelock, DP (reprint author), POB 578,300 E Coll Dr, Mesilla Pk, NM 88047 USA. EM derek.whitelock@ars.usda.gov FU Cotton Incorporated FX The authors would like to thank Cotton Incorporated for funding this research and conducting the fiber analyses. NR 12 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2015 VL 31 IS 3 BP 399 EP 403 PG 5 WC Agricultural Engineering SC Agriculture GA CM3AR UT WOS:000357554000007 ER PT J AU Garbrecht, JD Zhang, XC AF Garbrecht, J. D. Zhang, X. C. TI SOIL EROSION FROM WINTER WHEAT CROPLAND UNDER CLIMATE CHANGE IN CENTRAL OKLAHOMA SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Climate change; Climate projections; Climate uncertainty; Conservation practices; Soil erosion; Winter wheat ID LINE SIMULATION CHARACTERISTICS; UNITED-STATES; CHANGE IMPACTS; MODEL; PRECIPITATION; TRENDS; EARTH; VARIABILITY; FORMULATION; SCENARIOS AB Effects of climate change on soil erosion from a winter wheat field were investigated to determine what conservation practices would be required under climate change to maintain future soil erosion at no more than today's rates. Global Circulation Models (GCM) climate projections for climate change scenario RCP8.5 in Central Oklahoma were used. Hydrologic processes, winter wheat growth, and soil erosion were simulated with the Water Erosion Prediction Project (WEPP) model, and daily precipitation and air temperature were generated with weather generator SYNTOR. Tillage alternatives included conventional tillage with and without terraces, conservation tillage with and without terraces, double cropping winter wheat and soy beans, no till, and conversion to perennial pasture. Findings suggested that in Central Oklahoma soil erosion from winter-wheat fields under conventional tillage would double by mid-century under climate change scenario RCP8.5 with an up to 30% increase in daily rainfall amount for the upper 5 percentile of storms. A switch to conservation tillage would offset, on average, most of the anticipated increase in soil erosion. However, the uncertainty range of GCM climate projections was large and the target reduction in soil erosion could not be achieved for several of the climate projections. To overcome the uncertainty range of simulated soil erosion, more effective conservation practices such as the combination of conservation tillage and terraces, no-till, and/or land use conversion to pasture must be considered Based on these findings, it is inferred that wide implementation of today's conservation programs and policies would likely suffice to offset the anticipated increase in soil erosion from winter wheat fields under the high greenhouse gas emission scenario. C1 [Garbrecht, J. D.; Zhang, X. C.] ARS, Grazinglands Res Lab, USDA, El Reno, OK USA. RP Garbrecht, JD (reprint author), USDA ARS, Grazinglands Res Lab, 7207 West Cheyenne Rd, El Reno, OK 73036 USA. EM jurgen.garbrecht@ars.usda.gov NR 61 TC 3 Z9 3 U1 11 U2 22 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2015 VL 31 IS 3 BP 439 EP 454 PG 16 WC Agricultural Engineering SC Agriculture GA CM3AR UT WOS:000357554000011 ER PT J AU Muck, RE Brink, GE Broderick, GA AF Muck, R. E. Brink, G. E. Broderick, G. A. TI EFFECTS OF SILO TYPE ON ENSILING ALFALFA SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Loss; Fermentation; Fiber; Protein; Silage; Silo ID NEUTRAL DETERGENT FIBER; SILAGE; DENSITIES AB Various silo types are used on dairy farms, but there is uncertainty as to how silo type affects silage losses and quality. The objective of this study was to compare three silo types, filled with alfalfa from the same fields and emptied simultaneously, relative to filling rates, dry matter (DM) losses, and silage quality. Similar trials were performed in two consecutive years where second cutting alfalfa was harvested in late June and ensiled in three silos: bunker (4.9 x 21.3 x 3.5 m), bag (2.4 m dia. x 52 m), and oxygen-limiting tower (4.3 m dia. x 15.2 m). Approximately half of the forage from each field in each trial was ensiled in the bunker silo, and a quarter was allocated to the bag and tower silos. All three silos were filled over approximately a 24-h period with target DM concentrations of 35% to 40% for the bag and bunker silos and 45% to 50% for the tower. For each load, weight and times for filling and packing were recorded; a sample was taken for DM and nutrient analysis. The following summer, all three silos were emptied over approximately the same dates. Daily silage samples from each silo were composited weekly for analysis, and the weights of all silage removed were recorded whether fed or not. Filling rates (Mg DM per h) were similar for all silo types using the established management practices and equipment at the research farm. Over the two trials, average DM losses from the bag, bunker, and oxygen-limiting silos were 11%, 17%, and 4%, respectively, for 14 to 15 months storage. There were few effects of silo type on nutritive characteristics such as crude protein (CP) and fiber fractions of the alfalfa silages in the first year when fermentation + respiration DM losses varied over a narrow range across silos. In the second year where the bunker silo had the greatest fermentation + respiration losses, CP was reduced and fiber fractions were increased in the bunker silage compared with those in the silages from the other two silos. Small effects in silage fermentation due to silo type were observed in the first year where the DM concentrations were similar across the three silos. In the second year, the best fermentation occurred in the oxygen-limited silo whereas the bunker silo with the greatest losses had evidence of clostridial fermentation beginning. C1 [Muck, R. E.; Brink, G. E.; Broderick, G. A.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Muck, RE (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM remuck@wisc.edu FU Engineered Stored Products Co. FX The authors thank Richard Walgenbach and his farm crew for harvesting and storing the feedstuffs used in these trials and Jill Davidson and her barn crew for pulling daily samples of the silages at the U.S. Dairy Forage Center Research Farm; Ursula Hymes-Fecht, Mary Becker, and Wendy Radloff for assisting with sampling and laboratory analyses. Partial financial support for this research from Engineered Stored Products Co. is also gratefully acknowledged. NR 17 TC 0 Z9 0 U1 4 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2015 VL 31 IS 3 BP 479 EP 486 PG 8 WC Agricultural Engineering SC Agriculture GA CM3AR UT WOS:000357554000014 ER PT J AU Singh, SP Miklas, PN AF Singh, Shree P. Miklas, Phillip N. TI Breeding Common Bean for Resistance to Common Blight: A Review SO CROP SCIENCE LA English DT Review ID PHASEOLUS-VULGARIS L; CAMPESTRIS PV. PHASEOLI; MARKER-ASSISTED SELECTION; BACTERIAL-BLIGHT; XANTHOMONAS-CAMPESTRIS; DISEASE RESISTANCE; GAMETE SELECTION; VAR.-FUSCANS; PHYSIOLOGICAL RESISTANCE; MOLECULAR MARKERS AB Common blight [caused by Xanthomonas campestris pv. phaseoli Smith (Dye)] is a major bacterial disease causing >40% seed yield and quality losses in common bean (Phaseolus vulgaris L.) worldwide. Use of resistant cultivars is crucial for its effective, economical, and environment friendly integrated management and control. Common blight resistant germplasm are found in the primary, secondary, and tertiary gene pools of the common bean. Substantial progress has been made in understanding the pathogenic variation, germplasm screening methods, identification of resistant germplasm, genetics of resistance, identifying and mapping molecular markers linked with resistance quantitative trait loci (QTL), introgressing resistance from the secondary and tertiary gene pools, transferring resistance from the Middle American common bean landraces to Andean cultivars and germplasm lines, and pyramiding or combining resistance from diverse germplasm sources into common bean. Backcross, pedigree, gamete, and recurrent selection methods or their modifications singularly or in combination have been used to introgress and pyramid resistance. Also, molecular markers with or without direct disease screening have been used for breeding for resistance. However, Andean and Middle American common bean cultivars with high levels of combined resistance to less-aggressive and aggressive bacterial strains in all aerial plant parts are not available. We will review progress achieved in breeding for resistance, briefly describe problems faced, and discuss strategies for integrated genetic improvement for common blight resistance for cultivar development. C1 [Singh, Shree P.] Univ Idaho, Plant Soil & Entomol Sci Dept, Kimberly, ID 83341 USA. [Miklas, Phillip N.] USDA ARS, Vegetable & Forage Crop Res Unit, Prosser, WA 99350 USA. RP Singh, SP (reprint author), Univ Idaho, Plant Soil & Entomol Sci Dept, 3793 North 3600 East, Kimberly, ID 83341 USA. EM singh@uidaho.edu NR 116 TC 0 Z9 0 U1 6 U2 26 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 971 EP 984 DI 10.2135/cropsci2014.07.0502 PG 14 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400001 ER PT J AU Fox, CM Cary, TR Nelson, RL Diers, BW AF Fox, Carolyn M. Cary, Troy R. Nelson, Randall L. Diers, Brian W. TI Confirmation of a Seed Yield QTL in Soybean SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCUS; GLYCINE-MAX L.; AGRONOMIC TRAITS; POPULATIONS; ALLELES; IDENTIFICATION; INTROGRESSION; CULTIVARS; GERMPLASM; MARKERS AB Exotic germplasm can be an important source of genetic diversity for soybean [Glycine max (L.) Merr.] improvement. Previously, four yield quantitative trait loci (QTL) had been identified in a cross between the exotic soybean plant introduction (PI) 68658 and the U.S. cultivar Lawrence. The confirmation of these QTL in other genetic backgrounds will provide further evidence of their usefulness in cultivar development. To confirm the four yield QTL, a population of 117 recombinant inbred lines (RILs) was developed from a backcross using a line from the initial PI 68658 mapping study as the donor parent and LD00-3309, a high-yielding elite cultivar, as the recurrent parent. The confirmation population was grown in Illinois at two locations in 2008, 2009, and 2010. One yield QTL, linked to marker Satt300 on chromosome (Chr) 5, was confirmed across the experiment (p < 0.01), and the high-yield allele was contributed by exotic parent PI 68658. This QTL was given the confirmed designation cqSeed yield-002. A second yield QTL, linked to Satt474 on Chr 14, was significant (p < 0.05) across the experiment, with the high-yield allele contributed by LD00-3309. Analysis of yield components from one location showed a significant increase in the total number of pods per plant associated with both of these markers. Our results provide evidence that valuable yield QTL exist in exotic soybean germplasm and that these QTL could be used to increase yield in modern cultivars. C1 [Fox, Carolyn M.; Cary, Troy R.; Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Genet Res Unit, USDA ARS, Urbana, IL 61801 USA. RP Diers, BW (reprint author), Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM bdiers@illinois.edu FU United Soybean Board FX This work was supported by funding from the United Soybean Board to Carolyn M. Fox. NR 32 TC 3 Z9 3 U1 1 U2 16 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 992 EP 998 DI 10.2135/cropsci2014.10.0688 PG 7 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400003 ER PT J AU Riday, H Reisen, P Raasch, JA Santa-Martinez, E Brunet, J AF Riday, Heathcliffe Reisen, Peter Raasch, John A. Santa-Martinez, Emmanuel Brunet, Johanne TI Selfing Rate in an Alfalfa Seed Production Field Pollinated with Leafcutter Bees SO CROP SCIENCE LA English DT Article ID FLORAL DISPLAY SIZE; AQUILEGIA CAERULEA RANUNCULACEAE; MEDICAGO-SATIVA L; CROSS-COMPATIBILITY; GENETIC DIVERSITY; MIMULUS-RINGENS; SET; FERTILIZATION; CULTIVARS; PATTERNS AB Alfalfa (Medicago sativa L.) self-pollination (i.e., selfing) causes inbreeding depression. Determining factors influencing alfalfa seed production selfing rates could inform potential mitigation strategies to reduce selfing. We measured in situ selfing rates from seed sampled from random plants in a commercial alfalfa seed production field pollinated by leafcutter bees (Megachile rotundata F.). Alfalfa selfing rates were estimated by genotyping similar to 24 progeny from each of 38 maternal plants. Maternal plant distance to pollinator domicile, pod position on racemes, raceme position on stems, and seeds per pod were noted during seed and tissue collection. Selfing rates averaged 11.8% with individual selfing rates ranging from 0% to 52.2%. Seed from pods collected from upper parts of racemes had lower selfing rates (9.1%) compared to pods from lower parts of racemes (15.1%). When "low" self-compatible (<15% selfing rate in 3+ seeded pods) and "high" self-compatible (>= 15% selfing rate in 3+ seeded pods) plants were examined separately, however, this pattern remained significant only for low self-compatible plants (upper raceme selfing rates 3.1% vs. lower raceme 8.3%). Low self-compatible plants had higher selfing rates in 1-2 seeded pods (12.9%) compared to 3+ seeded pods (3.8%) while high self-compatible plants showed no differences in selfing rates based on seed number per pod. Genetic differences for self-pollen's ability to outcompete outcross pollen when growing down the style best explained observed differences between low and high self-compatible plants. Best management practices and selection could help reduce but not eliminate selfing in alfalfa seed production fields. C1 [Riday, Heathcliffe; Raasch, John A.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Reisen, Peter] Forage Genet, Nampa, ID 83605 USA. [Santa-Martinez, Emmanuel] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Brunet, Johanne] Univ Wisconsin, Dept Entomol, VCRU, USDA ARS, Madison, WI 53706 USA. RP Riday, H (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM Heathcliffe.Riday@ars.usda.gov NR 36 TC 3 Z9 3 U1 4 U2 21 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1087 EP 1095 DI 10.2135/cropsci2014.04.0295 PG 9 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400012 ER PT J AU Jahufer, MZZ Casler, MD AF Jahufer, M. Z. Z. Casler, M. D. TI Application of the Smith-Hazel Selection Index for Improving Biomass Yield and Quality of Switchgrass SO CROP SCIENCE LA English DT Article ID EXPECTED GENETIC GAINS; PANICUM-VIRGATUM L.; ENVIRONMENT INTERACTIONS; DIVERGENT SELECTION; SECONDARY TRAITS; FAMILY SELECTION; POPULATIONS; HERITABILITY; LOWLAND; DIGESTIBILITY AB Apart from the importance of switchgrass (Panicum virgatum L.) as forage for livestock, it is useful as a high-value cellulosic biofuel feedstock. Breeding for a biofuel crop is complicated by the existence of multiple platforms for conversion of biomass to energy. Our main objective was to investigate the relative merits of single-trait selection, correlated response to selection, and Smith-Hazel-index-based selection for the following traits: biomass dry matter yield (YLD), ethanol (ETOH), Klason lignin (KL), and high heating value (HHV). The genetic analysis was based on a 2-yr data set generated from evaluation of 144 half-sib (HS) families in sward-plot trials at Arlington and Marshfield, WI. There was significant (P < 0.05) additive genetic variation among the families for all traits and for family x site interaction for the traits HHV and YLD. The estimates of narrow sense heritability on a HS family mean basis ranged from 0.37 for YLD to 0.51 for KL. Genetic correlation of YLD with ETOH, HHV, and KL were 0.38, 0.27, and 0.01, respectively. The index constructed to increase YLD and ETOH and reduce KL was most successful for a fermentation platform. This index enabled identification of families for enhancing ethanol production that would have been missed if selection was based solely on YLD. The index weighted to increase YLD and KL best suited a combustion platform. Both of these two indices had economic impacts superior to any other selection index evaluated. C1 [Jahufer, M. Z. Z.] AgResearch Ltd, Grasslands Res Ctr, Palmerston North, New Zealand. [Casler, M. D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Jahufer, MZZ (reprint author), AgResearch Ltd, Grasslands Res Ctr, Private Bag 11008, Palmerston North, New Zealand. EM zulfi.jahufer@agresearch.co.nz FU AgResearch Ltd.; Dairy New Zealand; USDA-ARS; USDA-NIFA Agriculture and Food Research Initiative [2011-68005-30411] FX The authors wish to acknowledge AgResearch Ltd. and Dairy New Zealand for supporting in part the sabbatical for Dr. Jahufer at the University of Wisconsin and the USDA-ARS, Madison, WI. We also wish to thank Mr. Brent Barrett, AgResearch Ltd., Grasslands Research Centre, for his encouragement and support, which made this sabbatical possible. This research was funded in part by congressionally allocated funds through USDA-ARS and by the USDA-NIFA Agriculture and Food Research Initiative Competitive Grant No. 2011-68005-30411 (CenUSA). NR 57 TC 5 Z9 5 U1 4 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1212 EP 1222 DI 10.2135/cropsci2014.08.0575 PG 11 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400024 ER PT J AU Pawlowski, ML Hill, CB Hartman, GL AF Pawlowski, M. L. Hill, C. B. Hartman, G. L. TI Resistance to Charcoal Rot Identified in Ancestral Soybean Germplasm SO CROP SCIENCE LA English DT Article ID MACROPHOMINA-PHASEOLINA RESISTANCE; MARKERS; GLYCINES; BIOLOGY; SORGHUM; MERRILL; GENES AB Charcoal rot, caused by the fungal pathogen Macrophomina phaseolina, is an economically important disease on soybean and other crops including maize (Zea mays L.), sorghum [Sorghum bicolor (L.) Moench], and sunflower (Helianthus annuus L.). Without effective cultural or chemical options to control charcoal rot in soybean [Glycine max (L.) Merr.], finding sources of genetic resistance is of high interest. In this study, 70 ancestral soybean genotypes were screened for resistance to M. phaseolina using a cut-stem inoculation technique under semi-controlled greenhouse conditions. Lesion progression on the stems in the first experiment was measured 7 to 15 d after inoculation. Three follow-up experiments were conducted to select and confirm the genotypes with the strongest partial resistance. Two experiments evaluated lesion lengths and the third experiment evaluated seedling survival. In the two experiments measuring lesion lengths, PI 548302 (42 and 38 mm) and PI 548414 (36 and 52 mm) had significantly shorter lesion lengths than the moderately resistant genotype, DT97-4290 (58 and 87 mm). In the fourth experiment, percentage survival of PI 548414 (88%), PI 548302 (81%), and PI 548178 (66%) were significantly higher than survival of DT97-4290 (32%). These three genotypes may be useful as parents for developing soybean cultivars with charcoal rot resistance. C1 [Pawlowski, M. L.; Hill, C. B.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, 1101 West Peabody Dr, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU United Soybean Board FX We thank Ms. T.K. Herman for her valuable review of the manuscript, Dr. Alemu Mengistu (USDA-ARS, Jackson, TN) for providing seed of soybean genotypes DT97-4290 and Pharaoh, Dr. Randall Nelson (USDA-ARS, Urbana, IL) for seeds from the USDA Soybean Germplasm Collection and Dr. John Rupe (University of Arkansas, Fayetteville, AR) for providing the M. phaseolina isolate, Pinetree. We would also like to thank the United Soybean Board for funding that supported this research. NR 31 TC 2 Z9 2 U1 0 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1230 EP 1235 DI 10.2135/cropsci2014.10.0687 PG 6 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400026 ER PT J AU Arelli, PR Mengistu, A Nelson, RL Cianzio, SR Vuong, T AF Arelli, Prakash R. Mengistu, Alemu Nelson, Randall L. Cianzio, Silvia R. Tri Vuong TI New Soybean Accessions Evaluated for Reaction to Heterodera glycines Populations SO CROP SCIENCE LA English DT Article ID CYST-NEMATODE; RESISTANCE; INHERITANCE; RACE-14 AB Soybean cyst nematode (SCN; Heterodera glycines Ichinohe) is a serious pest of soybean [Glycine max (L.) Merr.], in the United States and worldwide. Annual yield losses in the United States are estimated to be more than $1 billion. These losses have remained stable with the use of resistant cultivars, but over time nematodes will adapt to deployed resistance alleles. Currently used resistant cultivars do not have broad resistance. Soybean Plant Introduction (PI) 88788 and its derived cultivars continue to be the primary source of SCN resistance. It is important to identify new accessions with resistance to SCN as the first step to finding new alleles to provide long-term resistance. We have bioassayed in the greenhouse 100 newly available accessions from the USDA Soybean Germplasm Collection. We published methods to bioassay in the greenhouse, including culturing of nematodes to develop near-homogeneous populations for stable reactions on soybeans. Sixty-seven accessions with resistance reaction to Race 2 and/or Race 3 were identified, and SCN resistance was confirmed in replicated multiple tests. Among them, seventeen were yellow seeded. Soybean PIs that were yellow seeded and had resistance to both the races included PIs 512322D, 522186, and 567488B. These are the most desirable sources for development of soybean cultivars with resistance. These soybean lines will be evaluated for their genetic diversity to identify unique types to allow broadening of resistance gene introgression. C1 [Arelli, Prakash R.; Mengistu, Alemu] USDA ARS SEA, Crop Genet Res Unit, Jackson, TN 38301 USA. [Nelson, Randall L.] USDA ARS MWA, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. [Cianzio, Silvia R.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Tri Vuong] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Tri Vuong] Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA. RP Arelli, PR (reprint author), USDA ARS SEA, Crop Genet Res Unit, 605 Airways Blvd, Jackson, TN 38301 USA. EM prakash.arelli@ars.usda.gov FU United Soybean Board; Tennessee Soybean Promotion Board FX We appreciate the technical assistance of Dana Pekarchick and Susan Thomas in greenhouse bioassays. Funding for this research was supplemented by the United Soybean Board and the Tennessee Soybean Promotion Board, which is gratefully acknowledged. The use of trade, firm, or corporation names in this paper is for the information and convenience of the reader. Such use does not constitute an official endorsement of approval by the USDA-ARS, NAL, or BIC of any product or service to the exclusion of others that may be suitable. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or a part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, SW, Washington, DC 20250-9410 or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 22 TC 0 Z9 1 U1 4 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1236 EP 1242 DI 10.2135/cropsci2014.10.0696 PG 7 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400027 ER PT J AU Bonman, JM Babiker, EM Cuesta-Marcos, A Esvelt-Klos, K Brown-Guedira, G Chao, SM See, D Chen, JL Akhunov, E Zhang, JL Bockelman, HE Gordon, TC AF Bonman, J. Michael Babiker, Ebrahiem M. Cuesta-Marcos, Alfonso Esvelt-Klos, Kathy Brown-Guedira, Gina Chao, Shiaoman See, Deven Chen, Jianli Akhunov, Eduard Zhang, Junli Bockelman, Harold E. Gordon, Tyler C. TI Genetic Diversity among Wheat Accessions from the USDA National Small Grains Collection SO CROP SCIENCE LA English DT Article ID HEXAPLOID WHEAT; RESOURCES MANAGEMENT; LANDRACE ACCESSIONS; CORE COLLECTIONS; RESISTANCE; GERMPLASM; AESTIVUM; GENOME; COMMON AB Accessions of common wheat (Triticum aestivum L. subsp. aestivum) from the USDA-ARS National Small Grains Collection (NSGC) are a resource for wheat scientists worldwide. The genetic diversity of the wheat core subset, representing approximately 10% of the collection's 42,138 T. aestivum accessions, was examined using 390 diversity arrays technology (DArT) markers, 4941 single nucleotide polymorphisms (SNPs), and descriptor data. The marker profiles revealed duplicates, which were excluded to form an informative core (iCore) of 3230 accessions. The iCore population structure and diversity within various subgroups were examined with analysis of molecular variance, principal coordinate analysis, cluster analysis, and by ranking the contribution of individual accessions to overall diversity. Accession groups based on molecular marker data corresponded well to their geographic origin, and population structure was accounted for primarily by differences between Iranian landrace accessions and the rest of the accessions. Accessions classified as breeding lines were over represented among those ranked as most diverse based on SNP data, whereas Iranian landraces were under-represented. Although less diverse as a group, Iranian landrace accessions had a higher frequency of resistance to bunt diseases and Russian wheat aphid compared with the iCore as a whole. The present study provides support for establishing core subsets based on geographic origin of accessions and will be a basis for further study of diversity among NSGC wheats. C1 [Bonman, J. Michael; Babiker, Ebrahiem M.; Esvelt-Klos, Kathy; Bockelman, Harold E.; Gordon, Tyler C.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Cuesta-Marcos, Alfonso] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Brown-Guedira, Gina] N Carolina State Univ, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Chao, Shiaoman] USDA ARS, Genotyping Lab, Biosci Res Lab, Fargo, ND USA. [See, Deven] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Chen, Jianli] Univ Idaho, Aberdeen Res & Extens Ctr, Aberdeen, ID USA. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Zhang, Junli] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Bonman, JM (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, 1691 South 2700 West, Aberdeen, ID 83210 USA. EM mike.bonman@ars.usda.gov FU National Research Initiative from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This work was supported in part by National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture. We thank the many NSGC cooperators who generously contributed phenotypic data to GRIN over the past several decades and are grateful for the technical assistance of Anne Sturbaum and Amy Frazier. NR 31 TC 7 Z9 8 U1 1 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1243 EP 1253 DI 10.2135/cropsci2014.09.0621 PG 11 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400028 ER PT J AU Williams, MM AF Williams, Martin M., II TI Phenomorphological Characterization of Vegetable Soybean Germplasm Lines for Commercial Production SO CROP SCIENCE LA English DT Article ID MAX L. MERR.; SEED SIZE; WEED COMPETITION; GROWN EDAMAME; YIELD; PERFORMANCE; POPULATION; DIVERSITY; EMERGENCE; TRAITS AB Growing demand for vegetable soybean [Glycine max (L.) Merr.] has renewed interest in producing the crop in the United States, a significant importer of vegetable soybean despite being the world's largest producer and exporter of grain-type soybean. Field studies were conducted over 3 yr to(i) compare phenomorphological traits of vegetable and grain-type soybean and (ii) identify candidate lines for vegetable soybean production in the North Central United States, the nation's leading soybean-producing region. A total of 136 vegetable soybean entries from 22 sources were compared to 14 grain-type cultivars representing a range of maturity groups. Germination and emergence of vegetable soybean were poorer than grain-type entries. Seedling growth traits and rate of phenological development were higher in vegetable soybean. However, by the time of crop harvest (i.e., R6 growth stage), vegetable soybean produced shorter, smaller plants than grain-type soybean. Seed mass accounted for some of the variation in emergence and seedling traits. Filtering entry responses by criteria essential to viable commercial production, including a sensory evaluation, 12 entries from eight seed sources were identified as the most promising candidate lines for use in the North Central United States. By comparing vegetable soybean responses to grain-type soybean, this work puts into perspective the agronomic performance of vegetable soybean germplasm available to growers in the United States and points to specific areas of future research and crop development. C1 Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM mmwillms@illinois.edu NR 22 TC 0 Z9 0 U1 3 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1274 EP 1279 DI 10.2135/cropsci2014.10.0690 PG 6 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400031 ER PT J AU Brink, GE Sanderson, MA Casler, MD AF Brink, G. E. Sanderson, M. A. Casler, M. D. TI Grass and Legume Effects on Nutritive Value of Complex Forage Mixtures SO CROP SCIENCE LA English DT Article ID NEUTRAL DETERGENT FIBER; PRIMARY SPRING GROWTH; SPECIES-DIVERSITY; BIRDSFOOT-TREFOIL; KURA CLOVER; QUALITY; PRODUCTIVITY; COMMUNITIES; ALFALFA; YIELD AB Dry matter productivity of complex forage mixtures is typically influenced by a dominant species, but nutritive value may be a function of multiple components. Two experiments were conducted in Pennsylvania and Wisconsin during 2 yr. Each experiment consisted of 15 mixtures and monocultures of orchardgrass (Dactylis glomerata L.), quackgrass (Elymus repens (L.) Gould), alfalfa (Medicago sativa L.), and white clover (Trifolium repens L.), or of meadow fescue [Schedonorus pratensis (Huds.) P. Beauv.], reed canarygrass (Phalaris arundinacea L.), red clover (Trifolium pratense L.), and kura clover (Trifolium ambiguum M. Bieb.). Vegetative-stage mixtures and monocultures were harvested five times each year. Botanical composition was used to calculate the additive effect of each species, or the expected change in nutritive value associated with a change in proportion of a species in the mixture from 0 to 100%. Nutritive value differences among mixtures attributed to abundance of sown species were evident the first year, but except for monocultures, had largely disappeared in the second year. White clover and meadow fescue had a positive additive effect on forage digestibility (range of 36 to 80 g kg(-1)), but alfalfa and reed canarygrass had no additive effect on digestibility. Legumes generally had a negative additive effect (range of -179 to -81 g kg(-1)) and grasses a positive effect (range of 81 to 166 g kg(-1)) on forage neutral detergent fiber concentration. The results suggest that species with high nutritive value relative to other species within the same functional group have a disproportionate effect on nutritive value of complex mixtures. C1 [Brink, G. E.; Casler, M. D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Sanderson, M. A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Brink, GE (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM Geoffrey.Brink@ars.usda.gov NR 44 TC 1 Z9 1 U1 20 U2 38 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1329 EP 1337 DI 10.2135/cropsci2014.09.0666 PG 9 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400038 ER PT J AU Grabber, JH Coblentz, WK Riday, H Griggs, TC Min, DH MacAdam, JW Cassida, KA AF Grabber, J. H. Coblentz, W. K. Riday, H. Griggs, T. C. Min, D. H. MacAdam, J. W. Cassida, K. A. TI Protein and Dry-Matter Degradability of European- and Mediterranean-Derived Birdsfoot Trefoil Cultivars Grown in the Colder Continental USA SO CROP SCIENCE LA English DT Article ID CONDENSED TANNIN CONCENTRATION; LOTUS-CORNICULATUS L.; FORAGE QUALITY; RED-CLOVER; IN-SITU; CHEMICAL-COMPOSITION; DETERGENT FIBER; ALFALFA; YIELD; GERMPLASM AB Recent work suggests several European- and Mediterranean-derived cultivars of birdsfoot trefoil (BFT, Lotus corniculatus L.) are well adapted to the colder continental United States and produce forage with greater condensed tannin (CT) concentrations, but comparable neutral detergent fiber (NDF) and slightly lower crude-protein (CP) concentrations than the commonly grown cultivar Norcen. In the current study, thirteen of these foreign-derived cultivars and Norcen were harvested under two-or three-cut management during 2006 in Michigan, Utah, Wisconsin, and West Virginia and analyzed in vitro for rumen degradable and undegradable protein on a CP and dry-matter (DM) basis (RDP CP, RDP DM, and RUP DM) and for rumen degradable and true degradable DM (RDDM and TDDM). Foreign-derived cultivars usually produced forage with greater RUP DM and lower RDP CP, RDP DM, RDDM, and TDDM than Norcen. The low NDF cultivar Bokor uniquely combined high CT and RUP DM with high TDDM. The highest yielding cultivar Lotar also produced herbage with moderate to high CT, RUP DM, and TDDM. Concentrations of CT and CP influenced RDP CP, RDP DM, and RDDM, while CT affected RUP DM, and fiber components influenced TDDM; relationships were greatly influenced by growth environment. Reductions in RDP DM exceeded gains in RUP DM as CT increased, thus, feeding of high-CT cultivars could mainly curb urinary N excretion from excess RDP DM rather than boost amino acid supply from potentially digestible RUP DM. Additional work is needed to improve the nutritional consistency of harvested BFT across environments and to develop higher-yielding cultivars with optimal RDP DM and RUP DM and high TDDM for maximizing livestock performance. C1 [Grabber, J. H.; Riday, H.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Coblentz, W. K.] USDA ARS, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. [Griggs, T. C.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Min, D. H.] Kansas State Univ, Throckmorton Plant Sci Ctr 2004, Dept Agron, Manhattan, KS 66506 USA. [MacAdam, J. W.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. [Cassida, K. A.] Michigan State Univ, Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Grabber, JH (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr W, Madison, WI 53706 USA. EM John.Grabber@ars.usda.gov NR 42 TC 1 Z9 1 U1 2 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2015 VL 55 IS 3 BP 1356 EP 1364 DI 10.2135/cropsci2014.09.0659 PG 9 WC Agronomy SC Agriculture GA CM6DY UT WOS:000357779400041 ER PT J AU Ayers, CR Hanson-Dorr, KC O'Dell, S Lovell, CD Jones, ML Suckow, JR Dorr, BS AF Ayers, Christopher R. Hanson-Dorr, Katie C. O'Dell, Sadie Lovell, Charles D. Jones, Michael L. Suckow, Jason R. Dorr, Brian S. TI Impacts of colonial waterbirds on vegetation and potential restoration of island habitats SO RESTORATION ECOLOGY LA English DT Article DE double-crested cormorant; Great Lakes; Phalacrocorax auritus; soil; woody plants ID DOUBLE-CRESTED CORMORANTS; PHALACROCORAX-AURITUS; MANAGEMENT; SURVIVAL; ONTARIO AB Colonial waterbirds have impacted forested island ecosystems throughout their breeding range, changing vegetation, and soil characteristics and bird communities. Our objectives were to (1) determine effects of three levels of colonial waterbird exclusion on overall vegetation diversity and growth, and survival of a candidate restoration species (black elderberry; Sambucus nigra canadensis); (2) investigate effects of different planting techniques on survival and growth of black elderberry; and (3) determine effects of waterbird colonization on soil chemistry. In 2012, we investigated effects of three levels of waterbird exclusion (none control plots [CON]; partial, which excluded waterbirds larger than gulls [PEX]; and full which excluded all waterbirds [FEX]) on bird use, existing vegetation growth and diversity, and survival of planted black elderberry on three islands in Door County, WI, Lake Michigan. In 2013, we evaluated survival of black elderberry established with four planting treatments within three waterbird exclusion treatments on two islands in 2013. We also compared soil chemistry characteristics between islands with and without nesting waterbirds for 2 years. Overall plant growth was greater in exclosures, but elderberry survival was similar among treatments. Soil replacement and weed suppression planting treatments did not affect survival, but generally increased overall elderberry biomass. Soil from nesting islands was more acidic and had greater nutrient concentrations than reference islands. Exclusion or removal of colonial nesting waterbirds from islands may improve overall vegetation growth, but successful restoration of woody vegetation may require significant soil manipulation and planting. C1 [Ayers, Christopher R.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Hanson-Dorr, Katie C.; Dorr, Brian S.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, Mississippi State, MS 39762 USA. [O'Dell, Sadie] US Fish & Wildlife Serv, Mayville, WI 53050 USA. [Lovell, Charles D.; Jones, Michael L.] Wildlife Serv, USDA, Waupun, WI 53963 USA. [Suckow, Jason R.] Wildlife Serv, USDA, Sun Prairie, WI 53590 USA. RP Dorr, BS (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Mississippi Field Stn, Mississippi State, MS 39762 USA. EM Brian.S.Dorr@aphis.usda.gov OI Dorr, Brian/0000-0001-6857-8560; Hanson-Dorr, Katie/0000-0003-4559-938X FU EPA's Great Lakes Restoration Initiative through USDA, Wildlife Services of Wisconsin FX This research was funded by the EPA's Great Lakes Restoration Initiative through the USDA, Wildlife Services of Wisconsin. We thank the many staff with USDA/WS-WI, USDA/WS - National Wildlife Research Center, USFWS, Horicon National Wildlife Refuge Complex, USFWS Ecological Services, and the private landowners that provided access to boat launches and islands. We thank Keith Crouse for assistance with soil testing. The conclusions in this article are those of the authors and do not necessarily represent the views of the USFWS. NR 29 TC 1 Z9 1 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAY PY 2015 VL 23 IS 3 BP 252 EP 260 DI 10.1111/rec.12169 PG 9 WC Ecology SC Environmental Sciences & Ecology GA CN0UJ UT WOS:000358129000007 ER PT J AU Laarmann, D Korjus, H Sims, A Kangur, A Kiviste, A Stanturf, JA AF Laarmann, Diana Korjus, Henn Sims, Allan Kangur, Ahto Kiviste, Andres Stanturf, John A. TI Evaluation of afforestation development and natural colonization on a reclaimed mine site SO RESTORATION ECOLOGY LA English DT Article DE ecosystem restoration; long-term monitoring plot; oil-shale; reclamation; Scots pine; silver birch ID POST-MINING LANDSCAPES; SPONTANEOUS SUCCESSION; TECHNICAL RECLAMATION; FOREST RESTORATION; SILVER BIRCH; BLACK ALDER; ECOSYSTEMS; ESTONIA; REVEGETATION; CONSERVATION AB Post-mining restoration sites often develop novel ecosystems as soil conditions are completely new and ecosystem assemblage can be spontaneous even on afforested sites. This study presents results from long-term monitoring and evaluation of an afforested oil-shale quarry in Estonia. The study is based on chronosequence data of soil and vegetation and comparisons are made to similar forest site-types used in forest management in Estonia. After site reclamation, soil development lowered pH and increased N, K, and organic C content in soil to levels similar to the common Hepatica forest site-type but P, total C, and pH were more similar to the Calamagrostis forest site-type. Vegetation of the restoration area differed from that on common forest sites; forest stand development was similar to the Hepatica forest-type. A variety of species were present that are representive of dry and wet sites, as well as infertile and fertile sites. It appears that novel ecosystems may be developing on post-mining reclaimed land in Northeast Estonia and may require adaptations to typical forest management regimes that have been based on site-types. Monitoring and evaluation gives an opportunity to plan further management activities on these areas. C1 [Laarmann, Diana; Korjus, Henn; Sims, Allan; Kangur, Ahto; Kiviste, Andres] Estonian Univ Life Sci, Inst Forestry & Rural Engn, Tartu, Estonia. [Kangur, Ahto; Stanturf, John A.] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, Athens, GA 30602 USA. RP Laarmann, D (reprint author), Estonian Univ Life Sci, Inst Forestry & Rural Engn, Tartu, Estonia. EM diana.laarmann@emu.ee RI Sims, Allan/A-5119-2009; Korjus, Henn/N-3919-2014 OI Sims, Allan/0000-0003-1312-6940; Korjus, Henn/0000-0001-8522-7869 FU Estonian Environmental Investment Centre; Estonian University of Life Sciences; Estonian Research Council [ETF8890, IUT21-04] FX The establishment of the long-term monitoring plots was supported by the Estonian Environmental Investment Centre, by the Estonian University of Life Sciences and by the Estonian Research Council (ETF8890, IUT21-04). We thank fieldwork and technical staff for their input and anonymous reviewers for valuable comments to the manuscript. NR 47 TC 2 Z9 2 U1 7 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAY PY 2015 VL 23 IS 3 BP 301 EP 309 DI 10.1111/rec.12187 PG 9 WC Ecology SC Environmental Sciences & Ecology GA CN0UJ UT WOS:000358129000013 ER PT J AU Bradford, SA Wang, YS Torkzaban, S Simunek, J AF Bradford, Scott A. Wang, Yusong Torkzaban, Saeed Simunek, Jiri TI Modeling the release of E-coli D21g with transients in water content SO WATER RESOURCES RESEARCH LA English DT Article ID UNSATURATED POROUS-MEDIA; COLLOID-FACILITATED TRANSPORT; FLOW CONDITIONS; PORE-SCALE; BACTERIA TRANSPORT; SOLUTION CHEMISTRY; HANFORD SEDIMENTS; VADOSE-ZONE; MOBILIZATION; INTERFACE AB Transients in water content are well known to mobilize colloids that are retained in the vadose zone. However, there is no consensus on the proper model formulation to simulate colloid release during drainage and imbibition. We present a model that relates colloid release to changes in the air-water interfacial area (A(aw)) with transients in water content. Colloid release from the solid-water interface (SWI) is modeled in two steps. First, a fraction of the colloids on the SWI partitions to the mobile aqueous phase and airwater interface (AWI) when the A(aw) increases during drainage. Second, colloids that are retained on the AWI or at the air-water-solid triple line are released during imbibition as the AWI is destroyed. The developed model was used to describe the release of Escherichia coli D21g during cycles of drainage and imbibition under various saturation conditions. Simulations provided a reasonable description of experimental D21g release results. Only two model parameters were optimized to the D21g release data: (i) the cell fraction that was released from the SWI (f(r)) and (ii) the cell fraction that partitioned from the SWI to the AWI (f(awi)). Numerical simulations indicated that cell release was proportional to f(r) and the initial amount of retention on the SWI and AWI. Drainage to a lower water content enhanced cell release, especially during subsequent imbibition, because more bacteria on the SWI were partitioned to the AWI and/or aqueous phase. Imbibition to a larger water content produced greater colloid release because of higher flow rates, and more destruction of the AWI (smaller A(aw)). Variation in the value of f(awi) was found to have a pronounced influence on the amount of cell release in both drainage and imbibition due to changes in the partitioning of cells from the SWI to the aqueous phase and the AWI. C1 [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Wang, Yusong; Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Torkzaban, Saeed] CSIRO Land & Water, Glen Osmond, SA, Australia. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. EM Scott.Bradford@ars.usda.gov RI Torkzaban, Saeed/G-7377-2013 OI Torkzaban, Saeed/0000-0002-5146-9461 FU Manure and Byproduct Utilization Project of the USDA-ARS [214] FX This research was supported by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. Mention of trade names and company names in this manuscript does not imply any endorsement or preferential treatment by the USDA. All data can be provided upon request from the corresponding author. NR 50 TC 5 Z9 5 U1 6 U2 18 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD MAY PY 2015 VL 51 IS 5 BP 3303 EP 3316 DI 10.1002/2014WR016566 PG 14 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA CM6XB UT WOS:000357833600016 ER PT J AU Li, W Wang, K Chen, L Johnson, JA Wang, SJ AF Li, Wei Wang, Kun Chen, Long Johnson, Judy A. Wang, Shaojin TI Tolerance of Sitophilus zeamais (Coleoptera: Curculionidae) to heated controlled atmosphere treatments SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Sitophilus zeamais; Tolerance; Temperature; Heating rate; Controlled atmosphere/heating block systems ID THERMAL-DEATH KINETICS; FRUIT-FLY DIPTERA; TRANSITELLA WALKER LEPIDOPTERA; POMONELLA L. LEPIDOPTERA; STORED-PRODUCT INSECTS; HEATING RATE; QUARANTINE TREATMENTS; SWEET CHERRIES; CODLING MOTH; MORTALITY AB Combination heat and controlled atmosphere (CA) postharvest phytosanitary treatments are environmentally friendly alternatives to chemical fumigants. A controlled atmosphere/heating block system (CA-HBS) was used to rapidly assess tolerances of adult maize weevil, Sitophilus zeamais, both under regular air (RA) and CA (1% O-2 and 15% CO2) conditions. In the RA treatment, thermal death kinetics for S. zeamais adults were determined at temperatures between 46 degrees C and 52 degrees C at a heating rate of 5 degrees C/min. The results showed that thermal death curves of S. zeamais adults followed a 0th-order kinetic reaction model. The required holding times for achieving 100% mortality were 165, 40, 14, and 4 min at 46, 48, 50 and 52 degrees C, respectively. The activation energy for killings. zeamais adults was 526.7 kJ/mol. The effects of CA at various temperature-time combinations and heating rates on insect mortality were evaluated. The mortality of S. zeamais adults was higher under CA treatment than in the heat treatment alone. The slowest heating rate (0.1 degrees C/min) achieved the highest insect mortality in CA treatments but lowest mortality in RA treatments. The information obtained from the CA-HBS can be used to develop combination heat and CA treatments against S. zeamais. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Li, Wei; Wang, Kun; Chen, Long; Wang, Shaojin] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Johnson, Judy A.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wang, Shaojin] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Wang, SJ (reprint author), Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. EM shaojinwang@nwsuaf.edu.cn FU Ph.D. Programs Foundation of Ministry of Education of China [20120204110022]; General Program of National Natural Science Foundation of China [31371853]; Shaanxi Agricultural Science and Technology Innovation and Research Project [2013K01-50] FX This research was supported by research grants from Ph.D. Programs Foundation of Ministry of Education of China (20120204110022), General Program of National Natural Science Foundation of China (31371853), and Shaanxi Agricultural Science and Technology Innovation and Research Project (2013K01-50). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 35 TC 4 Z9 5 U1 4 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD MAY PY 2015 VL 62 BP 52 EP 57 DI 10.1016/j.jspr.2015.04.001 PG 6 WC Entomology SC Entomology GA CL8KU UT WOS:000357224400009 ER PT J AU Song, LS Liu, SM Kustas, WP Zhou, J Ma, YF AF Song, Lisheng Liu, Shaomin Kustas, William P. Zhou, Ji Ma, Yanfei TI Using the Surface Temperature-Albedo Space to Separate Regional Soil and Vegetation Temperatures from ASTER Data SO REMOTE SENSING LA English DT Article ID ESTIMATE CROP EVAPOTRANSPIRATION; SPECTRAL MIXTURE ANALYSIS; COMPONENT TEMPERATURES; CANOPY TEMPERATURE; SATELLITE IMAGERY; TRIANGLE METHOD; BALANCE MODEL; LAND; MOISTURE; VALIDATION AB Soil and vegetation component temperatures in non-isothermal pixels encapsulate more physical meaning and are more applicable than composite temperatures. The component temperatures however are difficult to be obtained from thermal infrared (TIR) remote sensing data provided by single view angle observations. Here, we present a land surface temperature and albedo (T-alpha) space approach combined with the mono-surface energy balance (SEB-1S) model to derive soil and vegetation component temperatures. The T-alpha space can be established from visible and near infrared (VNIR) and TIR data provided by single view angle observations. This approach separates the soil and vegetation component temperatures from the remotely sensed composite temperatures by incorporating soil wetness iso-lines for defining equivalent soil temperatures; this allows vegetation temperatures to be extracted from the T-alpha space. This temperature separation methodology was applied to advanced scanning thermal emission and reflection radiometer (ASTER) VNIR and high spatial resolution TIR image data in an artificial oasis area during the entire growing season. Comparisons with ground measurements showed that the T-alpha space approach produced reliable soil and vegetation component temperatures in the study area. Low root mean square error (RMSE) values of 0.83 K for soil temperatures and 1.64 K for vegetation temperatures, respectively, were obtained, compared to component temperatures measurements from a ground-based thermal camera. These results support the use of soil wetness iso-lines to derive soil surface temperatures. It was also found that the estimated vegetation temperatures were extremely close to the near surface air temperature observations when the landscape is well watered under full vegetation cover. More robust soil and vegetation temperature estimates will improve estimates of soil evaporation and vegetation transpiration, leading to more reliable the monitoring of crop water stress and drought. C1 [Song, Lisheng; Liu, Shaomin; Ma, Yanfei] Beijing Normal Univ, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. [Song, Lisheng; Liu, Shaomin; Ma, Yanfei] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Song, Lisheng; Kustas, William P.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Zhou, Ji] Univ Elect Sci & Technol China, Sch Resources & Environm, Chengdu 611731, Peoples R China. [Ma, Yanfei] Handan Coll, Dept Geog, Handan 056005, Peoples R China. RP Liu, SM (reprint author), Beijing Normal Univ, State Key Lab Remote Sensing Sci, Beijing 100875, Peoples R China. EM scausls@163.com; smliu@bnu.edu.cn; Bill.Kustas@ars.usda.gov; jzhou233@uestc.edu.cn; mayanfei8866@126.com RI rslab, hiwater/O-7037-2015; rslab, water/O-7043-2015 FU National Natural Science Foundation of China [91125002]; National Basic Research Program of China [2015CB953702] FX We thank all the scientists, engineers, and students who participated in HiWATER field campaigns. This work was supported by the National Natural Science Foundation of China (91125002) and the National Basic Research Program of China (2015CB953702). We would like to thank Qiang Liu at College of Global Change and Earth System Science, Beijing Normal University. The authors also especially would like to thank Guoyu Qiu at School of Environment and Energy, Shenzhen Graduate School of Peking University for thermal images collected from the thermal camera and Mingsong Li at School of Resources and Environment, University of Electronic Science and Technology of China for supplying ASTER thermal infrared images in study area. We thank the anonymous referees for their constructive criticism and comments. NR 48 TC 2 Z9 2 U1 4 U2 23 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD MAY PY 2015 VL 7 IS 5 BP 5828 EP 5848 DI 10.3390/rs70505828 PG 21 WC Remote Sensing SC Remote Sensing GA CM3QC UT WOS:000357596900004 ER PT J AU Zhang, JM Huang, B Lu, XX Volk, GM Xin, X Yin, GK He, JJ Chen, XL AF Zhang, Jin-mei Huang, Bin Lu, Xin-xiong Volk, Gayle M. Xin, Xia Yin, Guang-kun He, Juan-juan Chen, Xiao-ling TI CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD SO CRYOLETTERS LA English DT Article DE Atractylodes macrocephala Koidz.; droplet-vitrification; regrowth; shoot tips; survival; thermal analysis ID APICAL MERISTEMS; CONSERVATION; POTATO; REGENERATION; ACCLIMATION; REGULATORS; RECOVERY; PROTOCOL; SURVIVAL; SUCROSE AB BACKGROUND: Atractylodes macrocephala Koidz. is an important medicinal species from China that has been used for thousands of years for its special pharmacological antioxidant, hepatoprotective, anti-inflammatory, anti-allergic, antithrombotic, antiviral, and anticarcinogenic activities. OBJECTIVE: The aim of this research was to develop an efficient droplet-vitrification protocol for A. macrocephala shoot tips which could be used as a strategy for long-term conservation within gene banks. MATERIALS AND METHODS: The duration of preculture, loading, and PVS2 steps, as well as the recovery medium formulation, were optimized to achieve high levels of survival and regrowth for A. macrocephala shoot tips after liquid nitrogen exposure. RESULTS: Survival and regrowth levels after cryopreservation in the cultivar 'Baizhu' were as high as 76% and 62%, respectively. Thermal analysis using differential scanning calorimetry suggested that the PVS2 treatment plays a critical role for successful cryopreservation. CONCLUSION: The droplet-vitrification method established in this study could be used to cryopreserve A. macrocephala. C1 [Zhang, Jin-mei; Lu, Xin-xiong; Xin, Xia; Yin, Guang-kun; He, Juan-juan; Chen, Xiao-ling] Chinese Acad Agr Sci, Inst Crop Sci, Natl Genebank, Beijing 100081, Peoples R China. [Huang, Bin] Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China. [Volk, Gayle M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Chen, XL (reprint author), Chinese Acad Agr Sci, Inst Crop Sci, Natl Genebank, Beijing 100081, Peoples R China. EM chenxiaoling@caas.cn FU International Science & Technology Cooperation Program of China [2014DFG1860]; Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS) [2014JB02-002]; Ministry of Agriculture [2014NWB030-11]; Agricultural Science and Technology Innovation Program (CAAS, ASTIP) FX This study was supported by International Science & Technology Cooperation Program of China (2014DFG1860), Core Research Budget of the Non-profit Governmental Research Institution (ICS, CAAS, 2014JB02-002), the Crop Germplasm Resources Protection and Utilization Special Grant from the Ministry of Agriculture (2014NWB030-11), and the Agricultural Science and Technology Innovation Program (CAAS, ASTIP). NR 52 TC 1 Z9 1 U1 0 U2 14 PU CRYO LETTERS PI LONDON PA C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND SN 0143-2044 EI 1742-0644 J9 CRYOLETTERS JI CryoLetters PD MAY-JUN PY 2015 VL 36 IS 3 BP 195 EP 204 PG 10 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA CM3AA UT WOS:000357552300007 PM 26510338 ER PT J AU Kerr, BJ Weber, TE Ziemer, CJ AF Kerr, B. J. Weber, T. E. Ziemer, C. J. TI Dietary marker effects on fecal microbial ecology, fecal VFA, nutrient digestibility coefficients, and growth performance in finishing pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE digestibility; indigestible markers; microbial ecology; pigs; volatile fatty acids ID APPARENT DIGESTIBILITY; GROWING PIGS; ILEAL AB Use of indigestible markers such as Cr2O3, Fe2O3, and TiO2 are commonly used in animal studies to evaluate digesta rate of passage and nutrient digestibility. Yet, the potential impact of indigestible markers on fecal microbial ecology and subsequent VFA generation is not known. Two experiments utilizing a total of 72 individually fed finishing pigs were conducted to describe the impact of dietary markers on fecal microbial ecology, fecal ammonia and VFA concentrations, nutrient digestibility, and pig performance. All pigs were fed a common diet with no marker or with 0.5% Cr2O3, Fe2O3, or TiO2. In Exp. 1, after 33 d of feeding, fresh fecal samples were collected for evaluation of microbial ecology, fecal ammonia and VFA concentrations, and nutrient digestibility, along with measures of animal performance. No differences were noted in total microbes or bacterial counts in pig feces obtained from pigs fed the different dietary markers while Archaea counts were decreased (P = 0.07) in feces obtained from pigs fed the diet containing Fe2O3 compared to pigs fed the control diet. Feeding Cr2O3, Fe2O3, or TiO2 increased fecal bacterial richness (P = 0.03, 0.01, and 0.10; respectively) when compared to pigs fed diets containing no marker, but no dietary marker effects were noted on fecal microbial evenness or the Shannon-Wiener index. Analysis of denaturing gradient gel electrophoresis gels did not reveal band pattern alterations due to inclusion of dietary markers in pig diets. There was no effect of dietary marker on fecal DM, ammonia, or VFA concentrations. Pigs fed diets containing Cr2O3 had greater Ca, Cu, Fe, and P (P = 0.02), but lower Ti (P = 0.08) digestibility compared to pigs fed the control diet. Pigs fed diets containing Fe2O3 had greater Ca (P = 0.08) but lower Ti (P = 0.01) digestibility compared to pigs fed the control diet. Pigs fed diets containing TiO2 had greater Fe and Zn (P = 0.09), but lower Ti (P = 0.01) digestibility compared to pigs fed the control diet. In Exp. 2, no effect of dietary marker on pig performance was noted. Overall, the data indicate that the inclusion of Cr2O3, Fe2O3, or TiO2 as digestibility markers have little to no impact on microbial ecology, fecal ammonia or VFA concentrations, nutrient digestibility, or pig growth performance indicating they are suitable for use in digestion studies. C1 [Kerr, B. J.; Weber, T. E.; Ziemer, C. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Kerr, BJ (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM brian.kerr@ars.usda.gov NR 16 TC 0 Z9 0 U1 0 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2015 VL 93 IS 5 BP 2183 EP 2190 DI 10.2527/jas.2014-8633 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6UO UT WOS:000357103200019 PM 26020314 ER PT J AU Freetly, HC Lindholm-Perry, AK Hales, KE Brown-Brandl, TM Kim, M Myer, PR Wells, JE AF Freetly, H. C. Lindholm-Perry, A. K. Hales, K. E. Brown-Brandl, T. M. Kim, M. Myer, P. R. Wells, J. E. TI Methane production and methanogen levels in steers that differ in residual gain SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; methane; methanogens ID GRADIENT GEL-ELECTROPHORESIS; FEEDING-BEHAVIOR; BOVINE RUMEN; CATTLE; PCR; EFFICIENCY; ENERGY; SHEEP; COMMUNITIES; RETENTION AB Methane (CH4) gas released by cattle is a product of fermentation in the digestive tract. The 2 primary sites of CH4 production in ruminants are the reticulum-rumen complex and the cecum. Methane release from cattle represents a 2% to 12% loss of the energy intake. Reducing the proportion of feed energy lost as CH4 has the potential of improving feed efficiency as well as decreasing the contribution of cattle to greenhouse gas production. Feed intake and growth were measured on 132 fall-born steers for 70 d. Seven steers with extreme positive residual gain (RG) and 7 steers with extreme negative RG whose DMI was within 0.32 SD of the mean intake were selected for subsequent measurements. Enteric CH4 production was measured via indirect calorimetry. Rumen, cecum, and rectal contents were obtained from steers at slaughter for measurement of in vitro CH4 production and methanogen 16S rRNA levels. Enteric CH4 production did not differ (P = 0.11) between the positive RG (112 +/- 13 L/d) and the negative RG (74 +/- 13 L/d) steers. In vitro rumen methane production did not differ between positive RG (64.26 x 10(-5) +/- 10.85 x 10(-5) mmol.g(-1) DM.min(-1)) and negative RG (61.49 x 10(-5) +/- 10.85 x 10(-5) mmol.g(-1) DM.min(-1); P = 0.86). In vitro cecum methane production did not differ between positive RG (4.24 x 10(-5) +/- 1.90 x 10(-5) mmol.g(-1) DM.min(-1)) and negative RG (4.35 x 10(-5) +/- 1.90 x 10(-5) mmol.g(-1) DM.min(-1); P = 0.97). Methanogen 16S rRNA as a percentage of the total bacteria 16S rRNA did not differ between RG groups (P = 0.18). The methanogen 16S rRNA as a percentage of rumen fluid total bacteria 16S rRNA (5.3% +/- 3.1%) did not differ from the methanogen 16S rRNA as a percentage of cecum content total bacteria 16S rRNA (11.8% +/- 3.1%; P = 0.14). The methanogen 16S rRNA as a percentage of the rectum content total bacteria 16S rRNA (0.7% +/- 3.1%) was not different from the rumen content (P = 0.29) but was less than the cecum content (P = 0.01). Methanomicrobiales 16S rRNA as a percentage of total methanogen 16S rRNA did not differ across sample sites (P = 0.81); however, steers with positive RG (10.5% +/- 1.6%) were more numerous than steers with negative RG (5.1% +/- 1.6%; P = 0.02). Cattle that differ in RG at the same DMI do not differ in characteristics associated with CH4 production. C1 [Freetly, H. C.; Lindholm-Perry, A. K.; Hales, K. E.; Brown-Brandl, T. M.; Kim, M.; Myer, P. R.; Wells, J. E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Freetly, HC (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM harvey.freetly@ars.usda.gov FU NIFA [2011-68004-30214] FX This project was partially funded by NIFA grant 2011-68004-30214 through the National Program for Genetic Improvement of Feed Efficiency in Beef Cattle. NR 29 TC 3 Z9 3 U1 2 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2015 VL 93 IS 5 BP 2375 EP 2381 DI 10.2527/jas.2014-8721 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6UO UT WOS:000357103200038 PM 26020333 ER PT J AU Agarwal, U Hu, Q Baldwin, RL Bequette, BJ AF Agarwal, U. Hu, Q. Baldwin, R. L. Bequette, B. J. TI Role of rumen butyrate in regulation of nitrogen utilization and urea nitrogen kinetics in growing sheep SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE butyrate; nitrogen; ruminant; urea; volatile fatty acids ID AMINO-ACID; SODIUM-BUTYRATE; METABOLISM; AMMONIA; BLOOD; GLUCOSE; TRACT AB Butyrate, a major rumen VFA, has been indirectly linked to enhancement of urea recycling on the basis of increased expression of urea transporter in the rumen epithelia of steers fed a rumen butyrate-enhancing diet. Two studies were conducted to quantify the effect of elevated rumen butyrate concentrations on N balance, urea kinetics and rumen epithelial proliferation. Wether sheep (n = 4), fitted with a rumen cannula, were fed a pelleted ration (similar to 165 g CP/kg DM, 10.3 MJ ME/kg DM) at 1.8 x ME requirement. In Exp. 1, sheep were infused intraruminally with either an electrolyte buffer solution (Con-Buf) or butyrate dissolved in the buffer solution (But-Buf) during 8-d periods in a balanced crossover design. In Exp. 2, sheep were infused intraruminally with either sodium acetate (Na-Ac) or sodium butyrate (Na-But) for 9 d. All solutions were adjusted to pH 6.8 and 8.0 in Exp. 1 and 2, respectively, and VFA were infused at 10% of ME intake. [N-15(2)] urea was continuously infused intravenously for the last 5 d of each period, and total urine and feces were collected. In Exp. 1, H-2(5)-phenylalanine was continuously infused intravenously over the last 12 h, after which a biopsy from the rumen papillae was taken for measurement of fractional protein synthesis rate (FSR). Butyrate infusion treatments increased (P = 0.1 in Exp. 1; P < 0.05 in Exp. 2) the proportion of rumen butyrate, and acetate infusion increased (P < 0.05) rumen acetate. All animals were in positive N balance (4.2 g N/d in Exp. 1; 7.0 g N/d in Exp. 2), but no difference in N retention was observed between treatments. In Exp. 2, urea entry (synthesis) rate was reduced (P < 0.05) by Na-But compared with the Na-Ac control. In Exp. 1, although But-Buf infusion increased the FSR of rumen papillae (35.3% +/- 1.08%/d vs. 28.7% +/- 1.08%/d; P < 0.05), urea kinetics were not altered by But-Buf compared with Con-Buf. These studies are the first to directly assess the role of butyrate in urea recycling and its effects on rumen papillae protein turnover in growing lambs. Under the feeding conditions used and the rate of continuous butyrate infusion into the rumen in the present studies, butyrate does not affect overall N retention in growing sheep. However, butyrate may play a role in the redistribution of urea N fluxes in the overall scheme of N metabolism. C1 [Agarwal, U.; Hu, Q.; Bequette, B. J.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20740 USA. [Baldwin, R. L.] USDA ARS, Anim Genom & Improvement Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Agarwal, U (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. EM umang.agarwal@bcm.edu FU Maryland Agricultural Experiment Station FX Funded by a grant from the Maryland Agricultural Experiment Station. NR 24 TC 4 Z9 5 U1 0 U2 11 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2015 VL 93 IS 5 BP 2382 EP 2390 DI 10.2527/jas.2014-8738 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6UO UT WOS:000357103200039 PM 26020334 ER PT J AU Brown, AC Powell, JG Kegley, EB Gadberry, MS Reynolds, JL Hughes, HD Carroll, JA Sanchez, NCB Thaxton, YV Backes, EA Richeson, JT AF Brown, A. C. Powell, J. G. Kegley, E. B. Gadberry, M. S. Reynolds, J. L. Hughes, H. D. Carroll, J. A. Sanchez, N. C. Burdick Thaxton, Y. V. Backes, E. A. Richeson, J. T. TI Effect of castration timing and oral meloxicam administration on growth performance, inflammation, behavior, and carcass quality of beef calves SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; castration; meloxicam ID PLASMA-CORTISOL; SURGICAL CASTRATION; LOCAL-ANESTHESIA; BULL CALVES; FEED-INTAKE; AGE; RESPONSES; CATTLE; KETOPROFEN AB Beef bull calves (n = 62) were assigned randomly, within sire breed, to 1 of 4 treatments at birth. Treatments were 1) surgical castration near birth, 2) surgical castration near birth with oral administration of meloxicam (1 mg/kg BW), 3) surgical castration at weaning (WNG), or 4) surgical castration at weaning with oral administration of meloxicam (1 mg/kg BW; WMX). A subset of calves (n = 7/treatment group) were selected randomly near birth for blood collection, behavioral analyses, and rectal temperature (RT) records for a 7-d postcastration period on d 0 (birth), 1, 3, and 7, and on d 214 (weaning), 214 + 6 h, 215, 217, 221, and 228. Calf standing and lying activity were monitored from the same subsets by recording x-and y-axis positions of an accelerometer attached to the right metatarsus for 7 d postcastration. Calf BW was recorded throughout the entire production cycle, and carcass data were collected at slaughter. For statistical analyses, bulls left intact at birth were considered a positive control (BUL) for observations that occurred before their treatment application at weaning; likewise, bulls castrated at birth were considered a negative control (STR) during postweaning observations. No difference (P >= 0.88) occurred in ADG between treatments throughout the preweaning period (d 0 to 214); however, 56-d postweaning ADG was greatest (P = 0.02) in STR, intermediate in WMX, and least in WNG. At weaning, haptoglobin (Hp) was greater (P = 0.005) for WNG and WMX compared to STR on d 214+ 6 h, 215, and 217, and Hp was greater (P <= 0.05) in WNG compared to WMX on d 217. Neutrophils increased (P < 0.001) and red blood cells decreased (P <= 0.03) for WNG and WMX on d 214+ 6 h and 217, respectively. Postweaning behavior observations indicated that STR calves spent the least proportion of time standing (P = 0.002) when compared to WNG and WMX. Furthermore, WMX calves exhibited a greater proportion of time spent standing (P = 0.03) compared to WNG. Grazing and finishing phase ADG and carcass measurements did not differ (P >= 0.24) across treatments. In this study, surgical castration at weaning, but not near birth, altered the acute phase response, behavior, and growth performance. Oral meloxicam reduced serum Hp and improved ADG briefly when administered to calves castrated at weaning. Oral administration of meloxicam may be efficacious for mitigating some of the stress and inflammation associated with castration of weaning-age bull calves. C1 [Brown, A. C.; Powell, J. G.; Kegley, E. B.; Reynolds, J. L.; Thaxton, Y. V.; Backes, E. A.] Univ Arkansas, Div Agr, Fayetteville, AR 72701 USA. [Gadberry, M. S.] Univ Arkansas, Div Agr, Little Rock, AR 72204 USA. [Hughes, H. D.; Richeson, J. T.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. [Carroll, J. A.; Sanchez, N. C. Burdick] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. RP Powell, JG (reprint author), Univ Arkansas, Div Agr, Fayetteville, AR 72701 USA. EM jerpow@uark.edu NR 22 TC 1 Z9 1 U1 7 U2 27 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2015 VL 93 IS 5 BP 2460 EP 2470 DI 10.2527/jas.2014-8695 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6UO UT WOS:000357103200046 PM 26020341 ER PT J AU Rotz, CA Asem-Hiablie, S Dillon, J Bonifacio, H AF Rotz, C. A. Asem-Hiablie, S. Dillon, J. Bonifacio, H. TI Cradle-to-farm gate environmental footprints of beef cattle production in Kansas, Oklahoma, and Texas SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef production; carbon footprint; energy use; environment; nitrogen loss; water use ID LIFE-CYCLE ASSESSMENT; PRODUCTION SYSTEMS; METHANE EMISSIONS; CARBON FOOTPRINT; MODEL; MITIGATION; EXCRETION AB A comprehensive national assessment of the sustainability of beef is being conducted by the U.S. beef industry. The first of 7 regions to be analyzed is Kansas, Oklahoma, and Texas. A survey and visits conducted throughout the region provided data on common production practices. From these data, representative ranch and feedyard operations were defined and simulated for the varying climate and soil conditions throughout the region using the Integrated Farm System Model. These simulations predicted environmental impacts of each operation including cradle-to-farm gate footprints for greenhouse gas emissions, fossil-based energy use, nonprecipitation water use, and reactive N loss. Individual ranch and feedyard operations were linked to form 28 representative production systems. A weighted average of the production systems was used to determine the environmental footprints for the region where weighting factors were developed based on animal numbers reported in the survey and agricultural statistics data. Along with the traditional beef production systems, Holstein steer and cull cow production from the dairy industry in the region were also modeled and included. The carbon footprint of all beef produced was 18.3 +/- 1.7 kg CO2 equivalents (CO2e)/kg carcass weight (CW) with the range in individual production systems being 13 to 25 kg CO2e/kg CW. Energy use, water use, and reactive N loss were 51 +/- 4.8 MJ/kg CW, 2,470 +/- 455 L/kg CW, and 138 +/- 12 g N/kg CW, respectively. The major portion of each footprint except water use was associated with the cow-calf phase; most of the nonprecipitation water use was attributed to producing feed for the finishing phase. These data provide a baseline for comparison as new technologies and strategies are developed and implemented to improve the sustainability of cattle production. Production information also will be combined with processing, marketing, and consumer data to complete a comprehensive life cycle assessment of beef. C1 [Rotz, C. A.; Asem-Hiablie, S.; Bonifacio, H.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Dillon, J.] Penn State Univ, Dept Anim Sci, University Pk, PA 16802 USA. RP Rotz, CA (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM al.rotz@ars.usda.gov FU Beef Checkoff; USDA's Agricultural Research Service FX Funded in part by The Beef Checkoff and the USDA's Agricultural Research Service. The authors thank K.R. Stackhouse and others of the National Cattlemen's Beef Association for their help in obtaining information supporting this analysis. The USDA is an equal opportunity provider and employer. NR 23 TC 2 Z9 2 U1 6 U2 40 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2015 VL 93 IS 5 BP 2509 EP 2519 DI 10.2527/jas.2014-8809 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6UO UT WOS:000357103200051 PM 26020346 ER PT J AU Andersen, WC Casey, CR Schneider, MJ Turnipseed, SB AF Andersen, Wendy C. Casey, Christine R. Schneider, Marilyn J. Turnipseed, Sherri B. TI Expansion of the Scope of AOAC First Action Method 2012.25-Single-Laboratory Validation of Triphenylmethane Dye and Leuco Metabolite Analysis in Shrimp, Tilapia, Catfish, and Salmon by LC-MS/MS SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID TANDEM MASS-SPECTROMETRY; MALACHITE GREEN; LIQUID-CHROMATOGRAPHY; GENTIAN-VIOLET; LEUCOMALACHITE GREEN; AQUACULTURE PRODUCTS; BRILLIANT GREEN; CRYSTAL VIOLET; FISH-TISSUES; RESIDUES AB Prior to conducting a collaborative study of AOAC First Action 2012.25 LC-MS/MS analytical method for the determination of residues of three triphenylmethane dyes (malachite green, crystal violet, and brilliant green) and their metabolites (leucomalachite green and leucocrystal violet) in seafood, a single-laboratory validation of method 2012.25 was performed to expand the scope of the method to other seafood matrixes including salmon, catfish, tilapia, and shrimp. The validation included the analysis of fortified and incurred residues over multiple weeks to assess analyte stability in matrix at -80 degrees C, a comparison of calibration methods over the range 0.25 to 4 mu g/kg, study of matrix effects for analyte quantification, and qualitative identification of targeted analytes. Method accuracy ranged from 88 to 112% with 13% RSD or less for samples fortified at 0.5, 1.0, and 2.0 mu g/kg. Analyte identification and determination limits were determined by procedures recommended both by the U.S. Food and Drug Administration and the European Commission. Method detection limits and decision limits ranged from 0.05 to 0.24 mu g/kg and 0.08 to 0.54 mu g/kg, respectively. AOAC First Action Method 2012.25 with an extracted matrix calibration curve and internal standard correction is suitable for the determination of triphenylmethane dyes and leuco metabolites in salmon, catfish, tilapia, and shrimp by LC-MS/MS at a residue determination level of 0.5 mu g/kg or below. C1 [Andersen, Wendy C.; Turnipseed, Sherri B.] US FDA, Off Regulatory Affairs, Anim Drugs Res Ctr, Denver, CO 80225 USA. [Casey, Christine R.] US FDA, Off Regulatory Affairs, Denver Lab, Denver, CO 80225 USA. [Schneider, Marilyn J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Andersen, WC (reprint author), US FDA, Off Regulatory Affairs, Anim Drugs Res Ctr, Denver Fed Ctr Bldg 20,W 6th Ave & Kipling St, Denver, CO 80225 USA. EM wendy.andersen@fda.hhs.gov NR 21 TC 1 Z9 1 U1 2 U2 6 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAY-JUN PY 2015 VL 98 IS 3 BP 636 EP 648 DI 10.5740/jaoacint.14-264 PG 13 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA CL4DS UT WOS:000356903400014 PM 26024871 ER PT J AU Schneider, MJ Andersen, WC AF Schneider, Marilyn J. Andersen, Wendy C. TI Determination of Triphenylmethane Dyes and Their Metabolites in Salmon, Catfish, and Shrimp by LC-MS/MS Using AOAC First Action Method 2012.25: Collaborative Study SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID MALACHITE GREEN; LEUCOMALACHITE GREEN; BRILLIANT GREEN; CRYSTAL VIOLET; RATS AB A collaborative study was conducted to evaluate the AOAC First Action 2012.25 LC-MS/MS analytical method for the determination of residues of three triphenylmethane dyes (malachite green, crystal violet, and brilliant green) and their metabolites (leucomalachite green and leucocrystal violet) in seafood. Fourteen laboratories from the United States, Canada, and the European Union member states participated in the study including national and state regulatory laboratories, university and national research laboratories, and private analytical testing laboratories. A variety of LC-MS/MS instruments were used for the analysis. Each participating laboratory received blinded test samples in duplicate of salmon, catfish, and shrimp consisting of negative control matrix; matrix fortified with residues at 0.42, 0.90, and 1.75 mu g/kg; and samples of incurred matrix. The analytical results from each participating laboratory were evaluated for both quantitative residue determination and qualitative identification of targeted analytes. Results from statistical analysis showed that this method provided excellent trueness (generally >= 90% recovery) and precision (RSDr generally <= 10%, HorRat <1). The Study Directors recommend Method 2012.25 for Final Action status. C1 [Schneider, Marilyn J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Andersen, Wendy C.] US FDA, Off Regulatory Affairs, Anim Drugs Res Ctr, Denver, CO 80225 USA. RP Andersen, WC (reprint author), US FDA, Off Regulatory Affairs, Anim Drugs Res Ctr, Denver Fed Ctr Bldg 20,W 6th Ave & Kipling St, Denver, CO 80225 USA. EM wendy.andersen@fda.hhs.gov NR 17 TC 2 Z9 2 U1 3 U2 5 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAY-JUN PY 2015 VL 98 IS 3 BP 658 EP 670 DI 10.5740/jaoacint.14-263 PG 13 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA CL4DS UT WOS:000356903400016 PM 26025133 ER PT J AU van Erp, H Shockey, J Zhang, M Adhikari, ND Browse, J AF van Erp, Harrie Shockey, Jay Zhang, Meng Adhikari, Neil D. Browse, John TI Reducing Isozyme Competition Increases Target Fatty Acid Accumulation in Seed Triacylglycerols of Transgenic Arabidopsis SO PLANT PHYSIOLOGY LA English DT Article ID DIACYLGLYCEROL ACYLTRANSFERASE; ENDOPLASMIC-RETICULUM; GENE; THALIANA; OIL; PLANTS; CASTOR; BIOSYNTHESIS; EXPRESSION; DGAT1 AB One goal of green chemistry is the production of industrially useful fatty acids (FAs) in crop plants. We focus on hydroxy fatty acids (HFAs) and conjugated polyenoic FAs (alpha-eleostearic acids [ESAs]) using Arabidopsis (Arabidopsis thaliana) as a model. These FAs are found naturally in seed oils of castor (Ricinus communis) and tung tree (Vernicia fordii), respectively, and used for the production of lubricants, nylon, and paints. Transgenic oils typically contain less target FA than that produced in the source species. We hypothesized that competition between endogenous and transgenic isozymes for substrates limits accumulation of unique FAs in Arabidopsis seeds. This hypothesis was tested by introducing a mutation in Arabidopsis diacylglycerol acyltransferase1 (AtDGAT1) in a line expressing castor FA hydroxylase and acyl-Coenzyme A:RcDGAT2 in its seeds. This led to a 17% increase in the proportion of HFA in seed oil. Expression of castor phospholipid:diacylglycerol acyltransferase 1A in this line increased the proportion of HFA by an additional 12%. To determine if our observations are more widely applicable, we investigated if isozyme competition influenced production of ESA. Expression of tung tree FA conjugase/desaturase in Arabidopsis produced approximately 7.5% ESA in seed lipids. Coexpression of VfDGAT2 increased ESA levels to approximately 11%. Overexpression of VfDGAT2 combined with suppression of AtDGAT1 increased ESA accumulation to 14% to 15%. Our results indicate that isozyme competition is a limiting factor in the engineering of unusual FAs in heterologous plant systems and that reduction of competition through mutation and RNA suppression may be a useful component of seed metabolic engineering strategies. C1 [van Erp, Harrie; Adhikari, Neil D.; Browse, John] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. [Shockey, Jay] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Zhang, Meng] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. RP Browse, J (reprint author), Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. EM jab@wsu.edu OI Shockey, Jay/0000-0002-5057-5457; Adhikari, Neil/0000-0002-2935-1870 FU U.S. National Science Foundation Plant Genome Research Program [DBI-0701919, IOS-1339385]; Agricultural Research Center at Washington State University FX This work was supported by the U.S. National Science Foundation Plant Genome Research Program (grant nos. DBI-0701919 and IOS-1339385) and the Agricultural Research Center at Washington State University. NR 49 TC 5 Z9 5 U1 1 U2 18 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD MAY PY 2015 VL 168 IS 1 BP 36 EP U687 DI 10.1104/pp.114.254110 PG 15 WC Plant Sciences SC Plant Sciences GA CL4ZO UT WOS:000356967800004 PM 25739701 ER PT J AU Limdi, NA Nolin, TD Booth, SL Centi, A Marques, MB Crowley, MR Allon, M Beasley, TM AF Limdi, Nita A. Nolin, Thomas D. Booth, Sarah L. Centi, Amanda Marques, Marisa B. Crowley, Michael R. Allon, Michael Beasley, T. Mark TI Influence of Kidney Function on Risk of Supratherapeutic International Normalized Ratio-Related Hemorrhage in Warfarin Users: A Prospective Cohort Study SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE Kidney function; chronic kidney disease (CKD); warfarin; supra-therapeutic international normalized ratio (INR); pharmacokinetics; hemorrhage; reversal of anticoagulation; adverse event ID CHRONIC-RENAL-FAILURE; K-DEPENDENT CARBOXYLATION; HEPATIC DRUG-METABOLISM; VITAMIN-K; ATRIAL-FIBRILLATION; OVERANTICOAGULATED PATIENTS; EXCESSIVE ANTICOAGULATION; COMPLICATIONS; DISEASE; PREDICTORS AB Background: Anticoagulation management is difficult in chronic kidney disease, with frequent supratherapeutic international normalized ratios (INRs >= 4) increasing hemorrhagic risk. We evaluated whether the interaction of INR and lower estimated glomerular filtration rate (eGFR) increases hemorrhage risk and whether patients with lower eGFRs experience slower anticoagulation reversal. Study Design: Prospective cohort study. Setting & Participants: Warfarin pharmacogenetics cohort (1,273 long-term warfarin users); warfarin reversal cohort (74 warfarin users admitted with INRs >= 4). Predictor: eGFR, INR as time-dependent covariate, and their interaction in the pharmacogenetics cohort; eGFR in the reversal cohort. Outcomes & Measurements: In the pharmacogenetics cohort, hemorrhagic (serious, life-threatening, and fatal bleeding) risk was assessed using proportional hazards regression. In the reversal cohort, anticoagulation reversal was assessed from changes in INR, warfarin and metabolite concentrations, clotting factors (II, VII, IX, and X), and PIVKA-II (protein induced by vitamin K absence or antagonist II) levels at presentation and after reversal, using linear regression and path analysis. Results: In the pharmacogenetics cohort, 454 (35.7%) had eGFRs, 60 mL/min/1.73 m(2). There were 137 hemorrhages in 119 patients over 1,802 person-years of follow-up (incidence rate, 7.6 [95% CI, 6.4-8.9]/100 person-years). Patients with lower eGFRs had a higher frequency of INR >= 4 (P < 0.001). Risk of hemorrhage was affected significantly by eGFR-INR interaction. At INR < 4, there was no difference in hemorrhage risk by eGFR (all P >= 0.4). At INR >= 4, patients with eGFRs of 30 to 44 and <30 mL/min/1.73 m(2) had 2.2-fold (95% CI, 0.8-6.1; P = 0.1) and 5.8-fold (95% CI, 2.9-11.4; P < 0.001) higher hemorrhage risks, respectively, versus those with eGFRs >= 60 mL/min/1.73 m(2). In the reversal cohort, 35 (47%) had eGFRs < 45 mL/min/1.73 m(2). Patients with eGFRs < 45 mL/min/1.73 m(2) experienced slower anticoagulation reversal as assessed by INR (P = 0.04) and PIVKA-II level (P = 0.008) than those with eGFRs >= 45 mL/min/1.73 m(2). Limitations: Limited sample size in the reversal cohort, unavailability of antibiotic use and urine albumin data. Conclusions: Patients with lower eGFRs have differentially higher hemorrhage risk at INR >= 4. Moreover, because the INR reversal rate is slower, hemorrhage risk is prolonged. (C) 2015 by the National Kidney Foundation, Inc. C1 [Limdi, Nita A.] Univ Alabama Birmingham, Neurol, Birmingham, AL 35294 USA. [Nolin, Thomas D.] Univ Pittsburgh, Sch Pharm, Pharm & Therapeut, Pittsburgh, PA USA. [Booth, Sarah L.; Centi, Amanda] Univ Pittsburgh, Sch Pharm, Vitamin K Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Pittsburgh, PA USA. [Marques, Marisa B.] Univ Alabama Birmingham, Pathol, Birmingham, AL USA. [Crowley, Michael R.] Univ Alabama Birmingham, Genet, Birmingham, AL USA. [Allon, Michael] Univ Alabama Birmingham, Div Nephrol, Med, Birmingham, AL USA. [Beasley, T. Mark] Univ Alabama Birmingham, Sect Stat Genet, Biostat, Birmingham, AL USA. RP Limdi, NA (reprint author), Univ Alabama Birmingham, Dept Neurol, 1235 Jefferson Tower,625 19th St S, Birmingham, AL 35294 USA. EM nlimdi@uab.edu OI Marques, Marisa/0000-0002-6689-7856 FU National Heart, Lung, and Blood Institute [RO1HL092173, RO1HL092173-S2]; National Institutes of Health Clinical and Translational Science Award program [UL1 TR000165]; US Department of Agriculture ARS [58-1950-7-707] FX This work was supported in part by grants from the National Heart, Lung, and Blood Institute (RO1HL092173 and RO1HL092173-S2), National Institutes of Health Clinical and Translational Science Award program (grant UL1 TR000165), and the US Department of Agriculture ARS Cooperative Agreement (58-1950-7-707). Stago Diagnostica provided test reagents/kits and consumables for analysis of clotting factors and PIVKA-II. The study sponsors had no role in the study design; collection, analysis, and interpretation of data; writing the manuscript; and the decision to submit the manuscript for publication. NR 47 TC 6 Z9 6 U1 0 U2 5 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0272-6386 EI 1523-6838 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD MAY PY 2015 VL 65 IS 5 BP 701 EP 709 DI 10.1053/j.ajkd.2014.11.004 PG 9 WC Urology & Nephrology SC Urology & Nephrology GA CL1SA UT WOS:000356723300012 PM 25468385 ER PT J AU Shelly, TE Nishimoto, JI AF Shelly, Todd E. Nishimoto, Jon I. TI Exposure to the Plant Compound alpha-Humulene Reduces Mating Success in Male Mediterranean Fruit Flies (Diptera: Tephritidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Mediterranean fruit fly; mating behavior; alpha-humulene ID GINGER ROOT OIL; CERATITIS-CAPITATA DIPTERA; MALASIACA THOMSON COLEOPTERA; SPOTTED LONGICORN BEETLE; ELECTROANTENNOGRAM RESPONSES; RELATIVE ATTRACTIVENESS; FLY; COMPETITIVENESS; COMMUNICATION; VOLATILES AB The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), displays lek mating, where searching females actively choose among aggregated males that produce visual, acoustic, and olfactory signals within the tree canopy. Recent studies demonstrated that treated males exposed to the aroma of particular plant compounds (alpha-copaene) or oils (orange, manuka, and ginger) gain a mating advantage over control, nonexposed males. The goal of this study was to assess the impact of another plant compound, alpha-humulene, on the mating success of male C. capitata. Prior work showed that alpha-humulene was not attractive to either sex but elicited a strong electroantennal response in males. Field cage tests showed that males exposed to the aroma of alpha-humulene obtained significantly fewer matings than control (nonexposed) males as long as 3d after exposure. Exposed males exhibited lower signaling (pheromone calling) activity than control males, which presumably contributed to their reduced mating success. Despite this lessened activity, the mortality of treated males after chemical exposure was similar to that observed for control, nonexposed males, suggesting that alpha-humulene was not a toxic or severely debilitating agent. C1 [Shelly, Todd E.; Nishimoto, Jon I.] USDA APHIS, Waimanalo, HI 96795 USA. RP Shelly, TE (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 34 TC 1 Z9 1 U1 3 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 215 EP 221 DI 10.1093/aesa/sav008 PG 7 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200001 ER PT J AU Handley, K Hough-Goldstein, J Hanks, LM Millar, JG D'Amico, V AF Handley, K. Hough-Goldstein, J. Hanks, L. M. Millar, J. G. D'Amico, V. TI Species Richness and Phenology of Cerambycid Beetles in Urban Forest Fragments of Northern Delaware SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Cerambycidae; attractant; phenology; forest fragmentation ID HOST-PLANT VOLATILES; COLEOPTERA CERAMBYCIDAE; LONGHORNED BEETLES; MEGACYLLENE-CARYAE; AGGREGATION PHEROMONE; SEX-PHEROMONE; BAITED TRAPS; ETHANOL; DIVERSITY; ECOLOGY AB Cerambycid beetles are abundant and diverse in forests, but much about their host relationships and adult behavior remains unknown. Generic blends of synthetic pheromones were used as lures in traps, to assess the species richness, and phenology of cerambycids in forest fragments in northern Delaware. More than 15,000 cerambycid beetles of 69 species were trapped over 2 yr. Activity periods were similar to those found in previous studies, but many species were active 1-3 wk earlier in 2012 than in 2013, probably owing to warmer spring temperatures that year. In 2012, the blends were tested with and without ethanol, a host plant volatile produced by stressed trees. Of cerambycid species trapped in sufficient numbers for statistical analysis, ethanol synergized pheromone trap catches for seven species, but had no effect on attraction to pheromone for six species. One species was attracted only by ethanol. The generic pheromone blend, especially when combined with ethanol, was an effective tool for assessing the species richness and adult phenology of many cerambycid species, including nocturnal, crepuscular, and cryptic species that are otherwise difficult to find. C1 [Handley, K.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. [Hanks, L. M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Millar, J. G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [D'Amico, V.] US Forest Serv, No Res Stn, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. RP Hough-Goldstein, J (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. EM jhough@udel.edu FU U.S. Department of Agriculture (USDA) Forest Service Northern Research Station FX Thanks to Jake Shaner, Lily Newton, Theresa Andrew, and Samantha Nestory for assistance in the field and laboratory. We also thank Judy Mongold-Diers for assistance in preparing pheromone lures, and Robert Naczi for help with identifying species, and designating new state records. Funding was provided by the U.S. Department of Agriculture (USDA) Forest Service Northern Research Station. NR 51 TC 10 Z9 10 U1 1 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 251 EP 262 DI 10.1093/aesa/sav005 PG 12 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200006 ER PT J AU Diaz-Montilla, AE Gonzalez, R Solis, MA Saldamando-Benjumea, CI AF Diaz-Montilla, A. E. Gonzalez, Ranulfo Solis, M. Alma Saldamando-Benjumea, C. I. TI Evidence of Sexual Selection in Neoleucinodes elegantalis (Lepidoptera: Crambidae): Correlation of Female Moth Genitalia and Solanaceae Host Fruit Size SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE selection; biotype; genitalia; Solanaceae; fruit ID MECHANICAL REPRODUCTIVE ISOLATION; GENETIC DIFFERENTIATION; PLANT ASSOCIATION; COLOMBIA; BORER; MORPHOLOGY; SPECIATION; NOCTUIDAE; PARAGUAY; INSECTS AB Neoleucinodes elegantalis (Guenee) (Lepidoptera: Crambidae) is a major pest of fruits in the family Solanaceae in the Western Hemisphere. The objectives of this study were to determine whether life zone or host plant explained morphological variation in females, and if so, if there was evidence of sexual selection driving diversification in this species. We collected larvae feeding on cultivated (Capsicum annuum L., Solanum betaceum Cavanilles, Solanum lycopersicum Lamarck, Solanum melongena L., and Solanum quitoense Lamarck) and wild species (Solanum atropurpureum Schrank, Solanum acerifolium Dunal, Solanum crinitum Lamarck, and Solanum hirtum Vahl) of Solanceae in Colombia. The genitalia traits of 547 reared females were measured and correlations with host plant fruit size were estimated. Six female genitalia morphological characters, apophysis posterioris, apophysis anterioris, ostium bursae, ductus bursae length, corpus bursae, and the seventh abdominal segment were measured. Principal component analysis and cluster analysis classified individuals based on female morphological similarity and clustered them into four main groups according to host plant: 1) S. aceriflolium; 2) S. quitoense, S. lycopersicum, C. annuum and S. hirtum; 3) S. atroporpureum; 4) S. melongena, S. crinitum and S. betaceum. In this unique study, we found that variation in female genitalia size is directly correlated with the size of its host fruit, which suggests a mechanism for reproductive isolation and divergence of the four host races. Ours is one of the first studies that shows female genitalia morphology is correlated with species of host plants and represents a valuable contribution to the study of sexual selection in the evolution of insects. C1 [Diaz-Montilla, A. E.] Corp Colombiana Invest Agr CORPOICA, Ctr Invest Selva, Rionegro, Antioquia, Colombia. [Gonzalez, Ranulfo] Univ Valle, Dept Biol, Cali, Colombia. [Solis, M. Alma] USDA ARS, Systemat Entomol Lab, PSI, Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. [Saldamando-Benjumea, C. I.] Univ Nacl Colombia Medellin, Dept Biociencias, Fac Ciencias, Medellin, Antioquia, Colombia. RP Diaz-Montilla, AE (reprint author), Corp Colombiana Invest Agr CORPOICA, Ctr Invest Selva, Rionegro, Antioquia, Colombia. EM anaelizabethd@gmail.com FU Ministerio de Agricultura y Desarrollo Rural de Colombia [PN25100033] FX Many thanks to Dr. Cesar Cardona (deceased) from Centro Internacional de Agricultura Tropical, International Center for Tropical Agriculture CIAT and to Dr. Argemiro Dominguez for statistics analysis. This project was funded by Ministerio de Agricultura y Desarrollo Rural de Colombia Project number PN25100033. NR 60 TC 2 Z9 2 U1 3 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 272 EP 281 DI 10.1093/aesa/sav011 PG 10 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200008 ER PT J AU Gomez, J Chavez, BY Castillo, A Valle, FJ Vega, FE AF Gomez, Jaime Chavez, Brenda Y. Castillo, Alfredo Valle, Francisco J. Vega, Fernando E. TI The Coffee Berry Borer (Coleoptera: Curculionidae): How Many Instars are There? SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE bark beetle; biology; broca del cafe; Hypothenemus; instar ID PINE-BEETLE COLEOPTERA; NUMBER; SCOLYTIDAE; WIDTHS AB After more than a century since the description of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), and dozens of scientific articles on the basic biology of the insect, there is still debate on the number of female larval instars. This article analyzes the metamorphosis of H. hampei females through direct observations during its entire biological cycle in the laboratory, together with scanning electron microscope photos. Also, the size of the head capsule of wild larvae and prepupae was analyzed with Dyar's rule and a discriminant analysis was conducted. Only two instars were observed during H. hampei metamorphosis up to the adult stage. Contrasting morphological changes in the larvae occurred when they transformed into prepupae, with no previous ecdysis. The statistical analysis revealed that the width of the cephalic masses form two significantly distinct groups before transformation into pupa, confirming that the prepupal stage forms part of the second larval instar. C1 [Gomez, Jaime; Chavez, Brenda Y.; Castillo, Alfredo; Valle, Francisco J.] El Colegio Frontera Sur, Tapachula 30700, Chiapas, Mexico. [Vega, Fernando E.] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. RP Gomez, J (reprint author), El Colegio Frontera Sur, Carretera Antiguo Aeropuerto Km 2-5, Tapachula 30700, Chiapas, Mexico. EM jgomez@ecosur.mx RI Alfredo, Castillo/B-5491-2016; Gomez, Jaime/O-1717-2016; OI Alfredo, Castillo/0000-0003-1452-0026; Gomez, Jaime/0000-0002-9704-9761; Vega, Fernando E./0000-0001-8103-5640 NR 31 TC 3 Z9 3 U1 0 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 311 EP 315 DI 10.1093/aesa/sav009 PG 5 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200012 ER PT J AU George, J Morse, WC Lapointe, SL AF George, Justin Morse, William C. Lapointe, Stephen L. TI Morphology and Sexual Dimorphism of the Weevil Myllocerus undecimpustulatus undatus (Coleoptera: Curculionidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Sri Lankan weevil; sexual dimorphism; Myllocerus undecimpustulatus undatus AB Myllocerus undecimpustulatus undatus Marshall (Coleoptera: Curculionidae), also known as the Sri Lankan weevil, is becoming a major pest of ornamentals and tropical fruit trees in the southern states of United States, especially in Florida. Recent findings of this species in Florida citrus groves justify research into their biology and ecology. Identification of semiochemicals involved in their behavioral and chemical communication will help to develop insect control strategies. We studied morphological and sexually dimorphic characters of this species as an aid to rapid separation of sexes for studies aiming to identify semiochemicals that may be of value in management. Female weevils collected from the field in southeastern Florida were significantly larger than males in length of head, abdomen, and overall length. Females weight was approximately twice that of males. No significant differences in the distance between procoxal or mesocoxal articulations observed between males and females. But the distance between the metacoxal articulations was significantly higher for females than males. All measures of female elytra were significantly greater than those of male elytra. Females, but not males, have a characteristic black-gray marking extending from the ventral mesosternum to the second abdominal segment. Scanning electron microscopic images revealed that females had fewer ovate-to-obovate scales in this region of characteristic black-gray marking and more plumose scales compared with males. Deposition of white cuticular hydrocarbons in this region was less dense on females compared with males. These characters proved sufficient to separate sexes 100% of the time in a blind test and should allow researchers to develop an accurate gestalt to separate males and females of the Sri Lankan weevil. C1 [George, Justin; Morse, William C.; Lapointe, Stephen L.] USDA ARS, US Hort Res Lab, Subtrop Insects & Hort Res Unit, Ft Pierce, FL 34945 USA. RP Lapointe, SL (reprint author), USDA ARS, US Hort Res Lab, Subtrop Insects & Hort Res Unit, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Stephen.Lapointe@ars.usda.gov NR 7 TC 1 Z9 1 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 325 EP 332 DI 10.1093/aesa/sav013 PG 8 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200014 ER PT J AU Armendariz-Toledano, F Nino, A Sullivan, BT Kirkendall, LR Zuniga, G AF Armendariz-Toledano, Francisco Nino, Alicia Sullivan, Brian T. Kirkendall, Lawrence R. Zuniga, Gerardo TI A New Species of Bark Beetle, Dendroctonus mesoamericanus sp nov (Curculionidae: Scolytinae), in Southern Mexico and Central America SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE bark beetle; Dendroctonus mesoamericanus; seminal rod; spermatheca ID FRONTALIS COLEOPTERA; PINE-BEETLE; MORPHOLOGY; KARYOLOGY; COMPLEX AB The bark beetle Dendroctonus mesoamericanus sp. nov. is described from a population in Parque Nacional Lagunas de Montebello, La Trinitaria, Chiapas, Mexico. This species belongs to the D. frontalis complex, which includes D. adjunctus Blandford 1897, D. approximatus Dietz 1890, D. brevicomis LeConte 1876, D. frontalis Zimmermann 1868, D. mexicanus Hopkins 1905, and D. vitei Wood 1975. The new species can be distinguished from closely related species D. frontalis, D. mexicanus, and D. vitei by four diagnostic morphological characters: the presence of striations on the preepisternal area of the prothorax (anterolateral pronotum) in both sexes, seminal rod with an approximately linear posterior margin of the dorsal process (in lateral view), irregularly sized ornamentations on the distal edge of squamiform plates present on the eighth abdominal tergite in females, and an inconspicuous pronotal callus in females. Furthermore, the karyotype of D. mesoamericanus sp. nov. (5AA_Xyp/XX) differs from that of its sister species D. frontalis (7AA_Xyp/XX). Dendroctonus mesoamericanus sp. nov. is distributed in Belize, Guatemala, El Salvador, Honduras, Nicaragua, and in the Michoacan, Oaxaca, and Chiapas states in Mexico. In these countries, this species has been collected at elevations above from 311 to 2600mon six pine species, and it commonly occurs in syntopy with D. frontalis. C1 [Armendariz-Toledano, Francisco; Zuniga, Gerardo] Escuela Nacl Ciencias Biol, Inst Politecn Nacl, Mexico City 11340, DF, Mexico. [Nino, Alicia] El Colegio Frontera ECOSUR, Chiapas 30700, Mexico. [Sullivan, Brian T.] USDA, Forest Serv, Southern Res Stn, Pineville, LA 71360 USA. [Kirkendall, Lawrence R.] Univ Bergen, Dept Biol, N-5006 Bergen, Norway. RP Zuniga, G (reprint author), Escuela Nacl Ciencias Biol, Inst Politecn Nacl, Mexico City 11340, DF, Mexico. EM capotezu@hotmail.com FU Secretaria de Investigacion y Posgrado IPN [20144216]; USDA Forest Service cooperative agreements FX We are grateful to Juan Jose Morrone, David Cibrian, and Miguel Angel Barrios for their comments on an earlier version of the manuscript. We thank Juan Cruz (Comision Nacional Forestal Mexico), Ivon Lopez (Comision Forestal Michoacana), Roberto Castellanos (Parque Nacional de Lagunas de Montebello, Mexico), Jorge Macias Samano (Synergy semiochemical Cooporation), Ronald Billings (Texas Forest Service), Steve Clarke (USDA Forest Service), and Fred Mitgaard and Karl H. Thunes (Norwegian Forest Research Institute) for their assistance in the field and laboratory, and for providing some specimens. This study was partially funded both by the Secretaria de Investigacion y Posgrado IPN (20144216) and USDA Forest Service cooperative agreements. F.A.T. was a member of Programa Institucional de Formacion de Investigadores of Instituto Politecnico Nacional (PIFI-IPN) and of Consejo Nacional de Ciencia y Tecnologia fellow (CONACyT 267436). NR 40 TC 4 Z9 4 U1 2 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 403 EP 414 DI 10.1093/aesa/sav020 PG 12 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200019 ER PT J AU Vega, FE Bauchan, G Infante, F Valdez-Carrasco, JM Beaver, R AF Vega, Fernando E. Bauchan, Gary Infante, Francisco Valdez-Carrasco, Jorge M. Beaver, Roger TI Visualizing the Mesothoracic Spiracles in a Bark Beetle: Hypothenemus hampei (Coleoptera: Curculionidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE broca del cafe; coffee berry borer; pygmy borers; Scolytinae ID ADULT SCARABAEOIDEA COLEOPTERA; PHYLOGENETIC SIGNIFICANCE AB In a low-temperature scanning electron microscopy study aimed at determining whether the coffee berry borer [Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae)] possesses mycangia, we fortuitously detected the mesothoracic spiracles, which are usually concealed. The mesothoracic spiracles are ventrolaterally located in the intersegmental membranes between the prothorax and mesothorax, and contain a large number of setae. This is the first study that includes photographs of the mesothoracic spiracles in a bark beetle. C1 [Vega, Fernando E.] ARS, USDA, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Bauchan, Gary] ARS, USDA, Elect & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Infante, Francisco] El Colegio Frontera ECOSUR, Tapachula 30700, Chiapas, Mexico. [Valdez-Carrasco, Jorge M.; Beaver, Roger] Colegio Postgrad, Montecillo 56230, Texcoco, Mexico. [Beaver, Roger] Roger Beaver, Chiang Mai 50180, Thailand. RP Vega, FE (reprint author), ARS, USDA, Sustainable Perennial Crops Lab, Bldg 001, Beltsville, MD 20705 USA. EM Fernando.Vega@ars.usda.gov OI Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 NR 10 TC 1 Z9 1 U1 2 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2015 VL 108 IS 3 BP 446 EP 448 DI 10.1093/aesa/sav016 PG 3 WC Entomology SC Entomology GA CL6NQ UT WOS:000357084200022 ER PT J AU Eiden, AL Kaufman, PE Oi, FM Allan, SA Miller, RJ AF Eiden, Amanda L. Kaufman, Phillip E. Oi, Faith M. Allan, Sandra A. Miller, Robert J. TI Detection of Permethrin Resistance and Fipronil Tolerance in Rhipicephalus sanguineus (Acari: Ixodidae) in the United States SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE brown dog tick; acaricide resistance; permethrin; fipronil ID BOOPHILUS-MICROPLUS ACARI; BROWN DOG TICK; PYRETHROID RESISTANCE; FLY DIPTERA; LATREILLE; COCKROACH; MUSCIDAE AB Permethrin is a commonly used acaricide for tick control on domestic animals and in residential environments, while fipronil use is restricted to on-animal treatment. Following widespread reports of permethrin and fipronil application failures to control indoor infestations of Rhipicephalus sanguineus (Latreille), the brown dog tick, 31 tick populations were obtained from Florida and Texas for acaricide resistance screening. These field-collected ticks from kennels and residential facilities were challenged with technical grade permethrin and fipronil to create dose response curves that were compared with an acaricide-susceptible strain. Permethrin resistance was successfully screened in nine populations, all of which were resistant or highly resistant. Tick susceptibility to fipronil was conducted on four populations, which were found to be tolerant, with resistance ratios below 10. This is the first documentation of R. sanguineus permethrin resistance and fipronil tolerance in the United States. Potential causes of resistance development and recommendations on future brown dog tick control management plans are discussed. C1 [Eiden, Amanda L.; Kaufman, Phillip E.; Oi, Faith M.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Allan, Sandra A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Miller, Robert J.] USDA ARS, Cattle Fever Tick Res Lab, Moore Air Base, Edinburg, TX 78541 USA. RP Eiden, AL (reprint author), Univ Florida, Dept Entomol & Nematol, 970 Nat Area Dr, Gainesville, FL 32611 USA. EM amandalynneiden@gmail.com FU U.S. Department of Agriculture Southern Region Integrated Pest Management program project [FLA-ENY-2009-34103-19907, 2009-41530-05595.] FX We thank Glenn Cawthorne (Ecto Services Inc., Henderson, NC) for providing the strain of susceptible ticks used in this study, Lois Wood, Emma Weeks, Lucas Carnohan, and the tick-contributing clientele and pest control operators who made this study possible. Funding for this study was provided by U.S. Department of Agriculture Southern Region Integrated Pest Management program project, FLA-ENY-2009-34103-19907 and 2009-41530-05595. NR 31 TC 7 Z9 7 U1 2 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2015 VL 52 IS 3 BP 429 EP 436 DI 10.1093/jme/tjv005 PG 8 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CL1RC UT WOS:000356720600017 PM 26334817 ER PT J AU Herrera, CS Rossman, AY Samuels, GJ Pereira, OL Chaverri, P AF Herrera, Cesar S. Rossman, Amy Y. Samuels, Gary J. Pereira, Olinto Liparini Chaverri, Priscila TI Systematics of the Cosmospora viliuscula species complex SO MYCOLOGIA LA English DT Article DE Ascomycota; fungal systematics; Hypocreales; mycoparasite; Nectria ID RAPID RADIATION; GENUS; PHYLOGENY; FUNGUS; BIONECTRIACEAE; HYPOCREALES; NECTRIACEAE; SPECIATION; ANAMORPHS; LINEAGE AB The genus Cosmospora includes nectrioid fungi that grow on polypores and xylariaceous fungi. The collections growing on xylariaceous fungi have been identified recently as Cosmospora viliuscula. In this paper the phylogeny and taxonomy of C. viliuscula are investigated. A phylogeny was generated with maximum likelihood and Bayesian inference methods applied to a three-partition dataset (ITS, 28S, MCM7-RPB1-TUB2). Based on these results, we demonstrate that Cosmospora viliuscula represents a diverse species complex comprising more than 10 species. Seven new species are described, including three single-strain lineages, and the sexual states of C. arxii and C. khandalensis are described for the first time. The sexual states of these fungi tend to have a high degree of morphological homoplasy, making it difficult to differentiate among them based on morphological characters alone. However, the apparent host specificity of species in this complex aide in the diagnosis of these fungi. In addition, the RPB1 marker provides sufficient resolution to distinguish these fungi. C1 [Herrera, Cesar S.; Chaverri, Priscila] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Rossman, Amy Y.; Samuels, Gary J.] USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Pereira, Olinto Liparini] Univ Fed Vicosa, Dept Fitopatol, BR-36570000 Vicosa, MG, Brazil. [Chaverri, Priscila] Univ Costa Rica, Escuela Biol, San Jose, Costa Rica. RP Herrera, CS (reprint author), Univ Maryland, Dept Plant Sci & Landscape Architecture, 2112 Plant Sci Bldg, College Pk, MD 20742 USA. EM csherrer@umd.edu FU United States National Science Foundation (NSF) PEET grant [DEB-0731510]; FAPEMIG; CNPq FX This study was supported by the United States National Science Foundation (NSF) PEET grant DEB-0731510 Monographic studies in the Nectriaceae, Hypocreales: Nectria, Cosmospora and Neonectria to P. Chaverri, A.Y. Rossman and G.J. Samuels. OLP acknowledges financial support from FAPEMIG and CNPq. We appreciate the fresh specimens sent to us from Canada by Keith A. Seifert. We thank the many people that helped us with collecting trips: A. Romero and R. Sanchez in Argentina; C. Mendez in Costa Rica; P. Johnston in New Zealand; and T. Iturriaga in Venezuela. We gratefully acknowledge the assistance of the curators and their staff of the herbaria from which specimens were generously loaned. These herbaria include: U.S. National Fungus Collection (BPI); Royal Botanic Gardens, Kew (K); William and Lynda Steere Herbarium, New York Botanical Garden (NY); the New Zealand Fungal and Plant Disease Collection (PDD); and Herbarium of the Botany Department, Swedish Museum of National History (S). NR 42 TC 2 Z9 3 U1 2 U2 7 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2015 VL 107 IS 3 BP 532 EP 557 DI 10.3852/14-122 PG 26 WC Mycology SC Mycology GA CL4EX UT WOS:000356906500009 PM 25800252 ER PT J AU Chaverri, P Branco-Rocha, F Jaklitsch, W Gazis, R Degenkolb, T Samuels, GJ AF Chaverri, Priscila Branco-Rocha, Fabiano Jaklitsch, Walter Gazis, Romina Degenkolb, Thomas Samuels, Gary J. TI Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains SO MYCOLOGIA LA English DT Article DE cryptic speciation; endophytes; fungal barcode; Hypocrea; phylogenetics; species concepts; taxonomy ID COMPETITIVE SAPROPHYTIC ABILITY; BIOLOGICAL-CONTROL; PHYLOGENETIC ANALYSES; MAXIMUM-LIKELIHOOD; FUNGUS TRICHODERMA; BAYESIAN-INFERENCE; AGARICUS-BISPORUS; THEOBROMA-CACAO; GREEN MOLD; EVOLUTION AB Trichoderma harzianum is known as a cosmopolitan, ubiquitous species associated with a wide variety of substrates. It is possibly the most commonly used name in agricultural applications involving Trichoderma, including biological control of plant diseases. While various studies have suggested that T. harzianum is a species complex, only a few cryptic species are named. In the present study the taxonomy of the T. harzianum species complex is revised to include at least 14 species. Previously named species included in the complex are T. guizhouense, T. harzianum, and T. inhamatum. Two new combinations are proposed, T. lentiforme and T. lixii. Nine species are described as new, T. afarasin, T. afroharzianum, T. atrobrunneum, T. camerunense, T. endophyticum, T. neotropicale, T. pyramidale, T. rifaii and T. simmonsii. We isolated Trichoderma cultures from four commercial biocontrol products reported to contain T. harzianum. None of the biocontrol strains were identified as T. harzianum s. str. In addition, the widely applied culture 'T. harzianum T22' was determined to be T. afroharzianum. Some species in the T. harzianum complex appear to be exclusively endophytic, while others were only isolated from soil. Sexual states are rare. Descriptions and illustrations are provided. A secondary barcode, nuc translation elongation factor 1-alpha (TEF1) is needed to identify species in this complex. C1 [Chaverri, Priscila] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Chaverri, Priscila] Univ Costa Rica, Escuela Biol, San Jose, Costa Rica. [Branco-Rocha, Fabiano] Empresa Pesquisa Agr & Extensao Rural Santa Catar, Estacao Expt Sao Joaquim, BR-88600000 Sao Joaquim, SC, Brazil. [Jaklitsch, Walter] Univ Vienna, Fac Ctr Biodivers, Dept Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Gazis, Romina] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Degenkolb, Thomas] Univ Giessen, Inst Nutr Sci, Dept Food Sci, Interdisciplinary Res Ctr BioSyst Land Use & Nutr, D-35392 Giessen, Germany. [Samuels, Gary J.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Chaverri, P (reprint author), Univ Maryland, Dept Plant Sci & Landscape Architecture, 2112 Plant Sci Bldg, College Pk, MD 20742 USA. EM pchaverr@umd.edu FU U.S. National Science Foundation (NSF) PEET grant [DEB-9712308]; NSF grants [DEB-925672, DEB-1019972]; Austrian Science Fund [P22081-B17]; Erwin-Stein-Foundation (Giessen, Germany) FX We appreciate many people who sent us cultures through the years. We especially thank Harry C. Evans (CABI, U.K) for providing endophytic cultures from Theobroma spp. and other hosts. Giovanni Vanacci (Pisa, Italy) provided many cultures from Europe. Authors are indebted to Roland Humm (Vitalin Pflanzengesundheit GmbH, Ober-Ramstadt, Germany) for providing a sample of Vitalin. Comments on certain aspects of the manuscript by Christian P. Kubicek (Vienna, Austria) are highly appreciated. Ed Ismaiel (AIRS, USDA) did some of the early sequencing that revealed some of the members of the T. harzianum complex included in the study. Lisa Casdebury (AIRS, USDA) arranged for deposit of cultures in CBS. This study was supported in part by the U.S. National Science Foundation (NSF) PEET grant DEB-9712308 to E.L. Stewart (Pennsylvania State University) and GJ.S.; and NSF grants DEB-925672 to P.C. and DEB-1019972 to P.C., K. Wurdack (Smithsonian NMNH), V. Pujade-Renaud (CIRAD), D. Garcia (CIRAD). WJ gratefully acknowledges the support by the Austrian Science Fund (project P22081-B17). TD is grateful for the support by a grant of the Erwin-Stein-Foundation (Giessen, Germany). NR 81 TC 14 Z9 19 U1 7 U2 37 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2015 VL 107 IS 3 BP 558 EP 590 DI 10.3852/14-147 PG 33 WC Mycology SC Mycology GA CL4EX UT WOS:000356906500010 PM 25661720 ER PT J AU Grupe, AC Baker, AD Uehling, JK Smith, ME Baroni, TJ Lodge, DJ Henkel, TW AF Grupe, Arthur C., II Baker, Anthony D. Uehling, Jessie K. Smith, Matthew E. Baroni, Timothy J. Lodge, D. Jean Henkel, Terry W. TI Sarcodon in the Neotropics I: new species from Guyana, Puerto Rico and Belize SO MYCOLOGIA LA English DT Article DE Bankeraceae; Caribbean; Central America; ectomycorrhizal fungi; Guiana Shield; Thelephorales; tooth fungi ID FUNGI; THELEPHORALES AB Four species of the ectomycorrhizal (ECM) genus Sarcodon (Bankeraceae, Thelephorales, Basidiomycota) are described as new to science. Sarcodon pakaraimensis sp. nov. is described from forests dominated by the ECM trees Pakaraimaea dipterocarpacea (Dipterocarpaceae) and Dicymbe jenmanii (Fabaceae subfam. Caesalpinioideae) in the Pakaraima Mountains of Guyana. Sarcodon portoricensis sp. nov. is described from lower montane wet forest within the El Yunque National Forest of Puerto Rico. Sarcodon quercophilus sp. nov. and Sarcodon umbilicatus sp. nov. are described from Quercus (Fagaceae) cloud forests within the Maya Mountains of Belize. The discovery of these species is significant given that the majority of the approximately 87 described Sarcodon species are north temperate or boreal in distribution and frequently associate with coniferous host plants; these constitute the most recent records for Sarcodon from the greater Neotropics. Each of the new species is morphologically consistent with accepted diagnostic characters for Sarcodon: pileate-stipitate stature, a dentate hymenophore, determinate basidiomatal development, fleshy, non-zonate context and brown, tuberculate basidiospores. DNA (ITS) sequence analysis corroborated the generic placement of S. pakaraimensis, S. portoricensis, S. quercophilus and S. umbilicatus and, along with morphological differences, supported their recognition as distinct species. Macromorphological, micromorphological, habitat and DNA sequence data from the nuc rDNA internal transcribed spacer region (ITS) are provided for each of the new species. A key to Neotropical Sarcodon species and similar extralimital taxa is provided. C1 [Grupe, Arthur C., II; Baker, Anthony D.; Henkel, Terry W.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. [Uehling, Jessie K.] Duke Univ, Univ Program Genet & Genom, Durham, NC 27708 USA. [Smith, Matthew E.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Baroni, Timothy J.] SUNY Coll Cortland, Dept Biol Sci, Cortland, NY 13045 USA. [Lodge, D. Jean] US Forest Serv, Ctr Forest Mycol Res, USDA, Forest Prod Lab, Luquillo, PR 00773 USA. RP Henkel, TW (reprint author), Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. EM twh5@humboldt.edu FU Humboldt Bay Mycological Association; Sonoma County Mycological Society Graduate Student Scholarship; California State University Program for Education and Research in Biotechnology; National Science Foundation [DEB-0918591, DEB-9525902, DEB-0103621]; National Geographic Society; British Mycological Society; University of Florida Institute for Food and Agricultural Sciences (IFAS) FX The authors thank the following for financial support: the George Verback Scholarship from the Humboldt Bay Mycological Association, Sonoma County Mycological Society Graduate Student Scholarship, and California State University Program for Education and Research in Biotechnology to ACG, National Science Foundation DEB-0918591 to TWH, and National Science Foundation-DEB-9525902 and DEB-0103621 to DJL and TJB for work in the Greater Antilles and Belize. The National Geographic Society and the British Mycological Society also provided support to TJB for mycological research in the Maya Mountains of Belize by helicopter. The Maya Mountains expedition was made possible by the logistical airlift support of the British Forces of Belize; Col Alan Whitelaw, the commanding officer of the Belize Forces and Ms Sharon Matola, director of the Belize Zoo, were responsible for making that expedition possible. Participation by MES was made possible by financial support from the University of Florida Institute for Food and Agricultural Sciences (IFAS). Field logistics in Puerto Rico were made possible by the USDA Forest Service, Center for Forest Mycology Research, Forest Products Laboratory. The CFMR facilities in Puerto Rico are maintained by the USDA Forest Service, International Institute of Tropical Forestry. Field assistance in Guyana was provided by C. Andrew, P. Joseph, F. Edmond and L. Edmond. Research permits were granted by the Guyana Environmental Protection Agency and Ministry of Amerindian Affairs. Dillon Husbands functioned as Guyanese local counterpart and assisted with field collecting, descriptions and specimen processing. Marty Reed assisted with the scanning electron micrographs. This paper is No. 205 in the Smithsonian Institution's Biological Diversity of the Guiana Shield Program publication series. NR 35 TC 3 Z9 3 U1 0 U2 2 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2015 VL 107 IS 3 BP 591 EP 606 DI 10.3852/14-185 PG 16 WC Mycology SC Mycology GA CL4EX UT WOS:000356906500011 PM 25661714 ER PT J AU Meyer, SE Pendleton, BK AF Meyer, Susan E. Pendleton, Burton K. TI Seedling establishment in a masting desert shrub parallels the pattern for forest trees SO ACTA OECOLOGICA-INTERNATIONAL JOURNAL OF ECOLOGY LA English DT Article DE Coleogyne ramosissima (blackbrush); Heteromyid rodent; Scatter-hoarding; Seed dispersal; Seed predation; Seedling bank ID COLEOGYNE-RAMOSISSIMA; SONORAN DESERT; EVOLUTIONARY ECOLOGY; PLANT-COMMUNITIES; BANK DYNAMICS; GERMINATION; COMPETITION; DEMOGRAPHY; ROSACEAE; RODENTS AB The masting phenomenon along with its accompanying suite of seedling adaptive traits has been well studied in forest trees but has rarely been examined in desert shrubs. Blackbrush (Coleogyne ramosissima) is a regionally dominant North American desert shrub whose seeds are produced in mast events and scatter-hoarded by rodents. We followed the fate of seedlings in intact stands vs. small-scale disturbances at four contrasting sites for nine growing seasons following emergence after a mast year. The primary cause of first-year mortality was post-emergence cache excavation and seedling predation, with contrasting impacts at sites with different heteromyid rodent seed predators. Long-term establishment patterns were strongly affected by rodent activity in the weeks following emergence. Survivorship curves generally showed decreased mortality risk with age but differed among sites even after the first year. There were no detectable effects of inter-annual precipitation variability or site climatic differences on survival. Intraspecific competition from conspecific adults had strong impacts on survival and growth, both of which were higher on small-scale disturbances, but similar in openings and under shrub crowns in intact stands. This suggests that adult plants preempted soil resources in the interspaces. Aside from effects on seedling predation, there was little evidence for facilitation or interference beneath adult plant crowns. Plants in intact stands were still small and clearly juvenile after nine years, showing that blackbrush forms cohorts of suppressed plants similar to the seedling banks of closed forests. Seedling banks function in the absence of a persistent seed bank in replacement after adult plant death (gap formation), which is temporally uncoupled from masting and associated recruitment events. This study demonstrates that the seedling establishment syndrome associated with masting has evolved in desert shrublands as well as in forests. Published by Elsevier Masson SAS. C1 [Meyer, Susan E.] US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. [Pendleton, Burton K.] US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, Albuquerque, NM 87106 USA. RP Meyer, SE (reprint author), US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, 735 N 500 East, Provo, UT 84606 USA. EM semeyer@xmission.com NR 43 TC 2 Z9 2 U1 9 U2 24 PU GAUTHIER-VILLARS/EDITIONS ELSEVIER PI PARIS PA 23 RUE LINOIS, 75015 PARIS, FRANCE SN 1146-609X EI 1873-6238 J9 ACTA OECOL JI Acta Oecol.-Int. J. Ecol. PD MAY-JUN PY 2015 VL 65-66 BP 1 EP 10 DI 10.1016/j.actao.2015.03.001 PG 10 WC Ecology SC Environmental Sciences & Ecology GA CK9JY UT WOS:000356557600001 ER PT J AU Johnson-Cicalese, J Polashock, JJ Honig, JA Vaiciunas, J Ward, DL Vorsa, N AF Johnson-Cicalese, Jennifer Polashock, James J. Honig, Josh A. Vaiciunas, Jennifer Ward, Daniel L. Vorsa, Nicholi TI Heritability of Fruit Rot Resistance American Cranberry SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE Vaccinium macrocarpon; genotyping; midparent-offspring regression; narrow sense heritability ID VACCINIUM-MACROCARPON; PHYLLOSTICTA-VACCINII AB Fruit rot is the primary threat to cranberry (Vaccinium macrocarpon) production in the northeastern United States, and increasingly in other growing regions. Efficacy of chemical control is variable because the disease is caused by a complex of pathogenic fungi. In addition, cranberries are often grown in environmentally sensitive areas, placing restrictions on chemical control measures. Thus, a major focus of the cranberry breeding program is to develop cultivars with improved fruit rot resistance (FRR). Several genetically diverse sources of FRR have been identified in our germplasm collection. However, the most resistant accessions lack one or more attributes; e.g., productivity, required for commercial acceptance. These resistant accessions were used in crosses with elite high-yielding selections and in 2009, 1624 progeny from 50 crosses were planted in 2.3-m(2) field plots. During 2011-13, under field conditions with very limited fungicide management, disease pressure was severe, allowing evaluation for FRR. Plots were rated on a 1-5 scale for incidence of fruit rot (where 1 =0% to 20% rot and 5 =81% to 100% rotted fruit), and rotted fruit counts were made from selected plots to validate the ratings. There was a good correlation in the ratings between years (2011 vs. 2012: r=0.59, P<0.0001; 2011 vs. 2013: r=0.50, P<0.0001; 2012 vs. 2013: r=0.62, P<0.0001), and between rot ratings and percent rotted fruit (r=0.90, P<0.0001). Significant differences were found between and within families for FRR. High heritability estimates (h(2) = 0.81) were obtained with midparent-offspring regression of mean fruit rot ratings, indicating additive genetic variance for FRR. Introgression of FRR into higher yielding genetic backgrounds was also accomplished, as some progeny exhibiting high FRR also had commercially viable yield (>300 g/0.09 m(2)), as well as good berry size and color. Selections are being further evaluated for potential cultivar release. C1 [Johnson-Cicalese, Jennifer; Vorsa, Nicholi] Rutgers State Univ, Dept Plant Biol & Pathol, PE Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. [Polashock, James J.] USDA ARS, GIFVL, PE Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. [Honig, Josh A.; Vaiciunas, Jennifer] Rutgers State Univ, Dept Plant Biol & Pathol, Sch Environm & Biol Sci, New Brunswick, NJ 08901 USA. [Ward, Daniel L.] Rutgers State Univ, Dept Plant Biol & Pathol, Rutgers Agr Res & Extens Ctr, Bridgeton, NJ 08302 USA. RP Vorsa, N (reprint author), Rutgers State Univ, Dept Plant Biol & Pathol, PE Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. EM vorsa@aesop.rutgers.edu FU USDA-SCRI [2008-51180-04878]; USDA National Institute of Food and Agriculture under Agriculture and Food Research Initiative Competitive Grant [USDA-NIFA-2013-67013-21107]; Ocean Spray Cranberries, Inc.; NJ Cranberry & Blueberry Research Council FX This project was supported by USDA-SCRI under Grant 2008-51180-04878; the USDA National Institute of Food and Agriculture under Agriculture and Food Research Initiative Competitive Grant USDA-NIFA-2013-67013-21107; Ocean Spray Cranberries, Inc.; and NJ Cranberry & Blueberry Research Council. NR 30 TC 1 Z9 1 U1 2 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAY PY 2015 VL 140 IS 3 BP 233 EP 242 PG 10 WC Horticulture SC Agriculture GA CL0KU UT WOS:000356632400004 ER PT J AU Dossett, M Bushakra, JM Gilmore, B Koch, CA Kempler, C Finn, CE Bassil, NV AF Dossett, Michael Bushakra, Jill M. Gilmore, Barbara Koch, Carol A. Kempler, Chaim Finn, Chad E. Bassil, Nahla V. TI Development and Transferability of Black and Red Raspberry Microsatellite Markers from Short-Read Sequences SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE Rubus idaeus; Rubus occidentalis; simple sequence repeat; SSR ID GENETIC DIVERSITY; RUBUS-OCCIDENTALIS; CHEMOPREVENTION; IDENTIFICATION; RESISTANCE; GENOME; PEACH AB The advent of next-generation, or massively parallel sequencing technologies has been a boon to the cost-effective development of molecular markers, particularly in nonmodel species. Here, we demonstrate the efficiency of microsatellite or simple sequence repeat (SSR) marker development from short-read sequences in black and red raspberry (Rubus occidentalis L. and R. idaeus L., respectively), compare transferability of markers across species, and test whether the rate of polymorphism in the recovered markers can be improved upon by how marker sequences are chosen. From 28,536,412 black raspberry reads and 27,430,159 reads in red raspberry, we identified more than 6000 SSR sequences in each species and selected 288 of these (144 from each species), for testing in black and red raspberry. A total of 166 SSR primer pairs were identified with informative polymorphism in one or both species. SSRs selected based on different percentages (90% to 97% as compared with >= 98%) of read cluster similarity did not differ in polymorphism rates from each other or from those originating from singletons. Efficiency of polymorphic SSR recovery was nearly twice as high in black raspberry from black raspberry-derived sequences as from red raspberry-derived sequences, while efficiency of polymorphic SSR recovery in red raspberry was unaffected by the source of the primer sequences. Development of SSR markers that are transferable between red and black raspberry for marker-assisted selection, evaluation of genome collinearity and to facilitate comparative studies in Rubus L. will be more efficient using SSR markers developed from black raspberry sequences. C1 [Dossett, Michael] BC Blueberry Council, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Bushakra, Jill M.; Bassil, Nahla V.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Gilmore, Barbara] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Koch, Carol A.; Kempler, Chaim] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Finn, Chad E.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Dossett, M (reprint author), BC Blueberry Council, Pacific Agri Food Res Ctr, POB 1000, Agassiz, BC V0M 1A0, Canada. EM Michael.Dossett@agr.gc.ca FU Lower Mainland Horticultural Improvement Association; Raspberry Industry Development Council of British Columbia; Washington Red Raspberry Commission; Oregon Raspberry and Blackberry Commission; Agriculture and Agri-Food Canada's Growing Forward and Growing Forward 2 Initiatives; USDA-National Institute of Food and Agriculture (NIFA) Specialty Crop Research Initiative (SCRI) Project [2011-51181-30676] FX This project was supported by funding from the Lower Mainland Horticultural Improvement Association, the Raspberry Industry Development Council of British Columbia, the Washington Red Raspberry Commission, and the Oregon Raspberry and Blackberry Commission, as well as Agriculture and Agri-Food Canada's Growing Forward and Growing Forward 2 Initiatives and USDA-National Institute of Food and Agriculture (NIFA) Specialty Crop Research Initiative (SCRI) Project 2011-51181-30676. Mention of trade names or commercial products in this publication is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 30 TC 0 Z9 0 U1 2 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAY PY 2015 VL 140 IS 3 BP 243 EP 252 PG 10 WC Horticulture SC Agriculture GA CL0KU UT WOS:000356632400005 ER PT J AU Yang, J Hirschi, KD Farmer, LM AF Yang, Jian Hirschi, Kendal D. Farmer, Lisa M. TI Dietary RNAs: New Stories Regarding Oral Delivery SO NUTRIENTS LA English DT Review ID MEDIATED GENE-REGULATION; DIGITAL PCR; GM FOOD; MICRORNAS; MIRNAS; EXPRESSION; PROFILES; XENOMIRS; ELEGANS; TARGETS AB microRNAs (miRNAs), a class of small RNAs, are important regulators of various developmental processes in both plants and animals. Several years ago, a report showed the detection of diet-derived plant miRNAs in mammalian tissues and their regulation of mammalian genes, challenging the traditional functions of plant miRNAs. Subsequently, multiple efforts have attempted to replicate these findings, with the results arguing against the uptake of plant dietary miRNAs in healthy consumers. Moreover, several reports suggest the potential for "false positive" detection of plant miRNAs in human tissues. Meanwhile, some research continues to suggest both the presence and function of dietary miRNAs in mammalian tissues. Here we review the recent literature and discuss the strengths and weaknesses of emerging work that suggests the feasibility of dietary delivery of miRNAs. We also discuss future experimental approaches to address this controversial topic. C1 [Yang, Jian; Hirschi, Kendal D.; Farmer, Lisa M.] Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. [Hirschi, Kendal D.] Texas A&M Univ, Vegetable & Fruit Improvement Ctr, College Stn, TX 77845 USA. RP Yang, J (reprint author), Baylor Coll Med, ARS, USDA, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. EM jiany@bcm.edu; kendah@bcm.edu; lmfarmer@bcm.edu RI Zhou, Man/S-4256-2016 FU USDA/ARIS [6250-51000-051-00D]; Soy Health Research Program; Dry Bean Health Research Program; NIH [5T32HD071839-02] FX This work was supported by USDA/ARIS 6250-51000-051-00D and funds from the Soy Health and Dry Bean Health Research Programs to KDH. LMF was supported by a grant from the NIH (5T32HD071839-02). We thank the many laboratories who have contributed to the field, both cited and not cited due to space limitations. We also thank Abia A. A. Agyekum and Ismail Elbaz for providing valuable input to the writing of the manuscripts. NR 46 TC 4 Z9 4 U1 0 U2 11 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD MAY PY 2015 VL 7 IS 5 BP 3184 EP 3199 DI 10.3390/nu7053184 PG 16 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CK5CT UT WOS:000356240500008 PM 25942490 ER PT J AU De Keyzer, W Bracke, T McNaughton, SA Parnell, W Moshfegh, AJ Pereira, RA Lee, HS van't Veer, P De Henauw, S Huybrechts, I AF De Keyzer, Willem Bracke, Tatiana McNaughton, Sarah A. Parnell, Winsome Moshfegh, Alanna J. Pereira, Rosangela A. Lee, Haeng-Shin van't Veer, Pieter De Henauw, Stefaan Huybrechts, Inge TI Cross-Continental Comparison of National Food Consumption Survey Methods-A Narrative Review SO NUTRIENTS LA English DT Review ID HABITUAL ENERGY-INTAKE; DOUBLY LABELED WATER; EPIC-SOFT SOFTWARE; NUTRITION SURVEY; VALIDATION EFCOVAL; EUROPEAN CENTERS; DIETARY SURVEY; RECALLS; HEALTH; INDICATORS AB Food consumption surveys are performed in many countries. Comparison of results from those surveys across nations is difficult because of differences in methodological approaches. While consensus about the preferred methodology associated with national food consumption surveys is increasing, no inventory of methodological aspects across continents is available. The aims of the present review are (1) to develop a framework of key methodological elements related to national food consumption surveys, (2) to create an inventory of these properties of surveys performed in the continents North-America, South-America, Asia and Australasia, and (3) to discuss and compare these methodological properties cross-continentally. A literature search was performed using a fixed set of search terms in different databases. The inventory was completed with all accessible information from all retrieved publications and corresponding authors were requested to provide additional information where missing. Surveys from ten individual countries, originating from four continents are listed in the inventory. The results are presented according to six major aspects of food consumption surveys. The most common dietary intake assessment method used in food consumption surveys worldwide is the 24-HDR (24 h dietary recall), occasionally administered repeatedly, mostly using interview software. Only three countries have incorporated their national food consumption surveys into continuous national health and nutrition examination surveys. C1 [De Keyzer, Willem; Bracke, Tatiana; De Henauw, Stefaan] Univ Coll Ghent, Fac Sci & Technol, Dept Bio & Food Sci, B-9000 Ghent, Belgium. [De Keyzer, Willem; De Henauw, Stefaan; Huybrechts, Inge] Univ Ghent, Dept Publ Hlth, Univ Hosp 4K3, B-9000 Ghent, Belgium. [McNaughton, Sarah A.] Deakin Univ, Sch Exercise & Nutr Sci, Ctr Phys Act & Nutr Res, Melbourne, Vic 3125, Australia. [Parnell, Winsome] Univ Otago, Div Sci, Dept Human Nutr, Dunedin 9054, New Zealand. [Moshfegh, Alanna J.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Pereira, Rosangela A.] Univ Fed Rio de Janeiro, Inst Nutr Josue de Castro, Dept Social Nutr, Ilha Fundao, BR-21941902 Rio De Janeiro, Brazil. [Lee, Haeng-Shin] Korea Hlth Ind Dev Inst, Nutr Management Serv & Policy Team, Chungbuk 363700, South Korea. [van't Veer, Pieter] Wageningen Univ, Div Human Nutr, NL-6703 HD Wageningen, Netherlands. [Huybrechts, Inge] Int Agcy Res Canc, F-69372 Lyon 08, France. RP De Keyzer, W (reprint author), Univ Coll Ghent, Fac Sci & Technol, Dept Bio & Food Sci, Campus Vesalius,Keramiekstr 80, B-9000 Ghent, Belgium. EM willem.dekeyzer@hogent.be; bracketatiana@gmail.com; sarah.mcnaughton@deakin.edu.au; winsome.parnell@otago.ac.nz; Alanna.Moshfegh@ars.usda.gov; rpereira@uol.com.br; haengsin@live.unc.edu; Pieter.vantVeer@wur.nl; stefaan.dehenauw@ugent.be; HuybrechtsI@iarc.fr RI McNaughton, Sarah/B-2075-2012 OI McNaughton, Sarah/0000-0001-5936-9820 FU University College Ghent; Australian Research Council [FT100100581] FX The authors gratefully acknowledge the contributions of the Health Statistics Division of Statistics Canada, Simon Barquera, Barry Popkin, Chris Killick-Moran and Shufa Du. The work presented was part of the PhD dissertation of W.D.K., who was financially supported by the Research Fund of University College Ghent. S.A.M. is funded by an Australian Research Council Future Fellowship (FT100100581). NR 53 TC 3 Z9 3 U1 1 U2 7 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD MAY PY 2015 VL 7 IS 5 BP 3587 EP 3620 DI 10.3390/nu7053587 PG 34 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CK5CT UT WOS:000356240500031 PM 25984745 ER PT J AU Raatz, SK Jahns, L Johnson, LK Crosby, R Mitchell, JE Crow, S Peterson, C Le Grange, D Wonderlich, SA AF Raatz, Susan K. Jahns, Lisa Johnson, LuAnn K. Crosby, Ross Mitchell, James E. Crow, Scott Peterson, Carol Le Grange, Daniel Wonderlich, Stephen A. TI Nutritional Adequacy of Dietary Intake in Women with Anorexia Nervosa SO NUTRIENTS LA English DT Article ID BULIMIA-NERVOSA; ENERGY-EXPENDITURE; EATING-DISORDERS; BEHAVIORS AB Understanding nutrient intake of anorexia nervosa (AN) patients is essential for the treatment. Therefore, estimates of total energy and nutrient consumption were made in a group of young women (19 to 30 years) with restricting and binge purge subtypes of AN participating in an ecological momentary assessment study. Participants completed three nonconsecutive 24-hour diet recalls. Mean nutrient intakes were stratified by subtype and by quartiles of energy intake and compared to the age specific Dietary Reference Intake (DRI) levels, as well as to the reported intakes from the What We Eat In America (WWEIA) dietary survey 2011-2012. Reported intake was determined for energy, macronutrients, and micronutrients. The mean body mass index (BMI) for all participants was 17.2 +/- 0.1 kg/m(2). Reported nutrient intake was insufficient for participants in quartiles 1-3 of both AN subtypes when compared to the DRIs. Intake reported by participants in quartile 4 of both subgroups met requirements for most nutrients and even met or exceeded estimated energy needs. Counseling of AN patients should be directed to total food consumption to improve energy intake and to reduce individual nutritional gaps. C1 [Raatz, Susan K.; Jahns, Lisa; Johnson, LuAnn K.] ARS, USDA, Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA. [Raatz, Susan K.; Crosby, Ross; Mitchell, James E.; Wonderlich, Stephen A.] Neuropsychiat Res Inst, Fargo, ND 58103 USA. [Crosby, Ross; Mitchell, James E.; Wonderlich, Stephen A.] Univ N Dakota, Dept Clin Neurosci, Grand Forks, ND 58202 USA. [Crow, Scott; Peterson, Carol] Univ Minnesota, Dept Psychiat, Sch Med, Minneapolis, MN 55455 USA. [Le Grange, Daniel] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA 94143 USA. [Le Grange, Daniel] Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA. RP Raatz, SK (reprint author), ARS, USDA, Human Nutr Res Ctr, Grand Forks, ND 58203 USA. EM susan.raatz@ars.usda.gov; lisa.jahns@ars.usda.gov; luann.johnson@ars.usda.gov; Rcrosby@nrifargo.com; Jmitchell@nrifargo.com; crowx002@umn.edu; peter161@umn.edu; Daniel.LeGrange@ucsf.edu; SWonderlich@nrifargo.com OI Crosby, Ross/0000-0001-9131-1629 FU National Institutes of Health [RO1-MH059674]; United States Department of Agriculture [3062-51000-051-00D, 3062-51000-053-00D] FX This work was funded by National Institutes of Health RO1-MH059674 and the United States Department of Agriculture 3062-51000-051-00D and 3062-51000-053-00D. NR 26 TC 3 Z9 3 U1 6 U2 15 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD MAY PY 2015 VL 7 IS 5 BP 3652 EP 3665 DI 10.3390/nu7053652 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CK5CT UT WOS:000356240500033 PM 25988761 ER PT J AU Aaron, GJ Dror, DK Yang, ZY AF Aaron, Grant J. Dror, Daphna K. Yang, Zhenyu TI Multiple-Micronutrient Fortified Non-Dairy Beverage Interventions Reduce the Risk of Anemia and Iron Deficiency in School-Aged Children in Low-Middle Income Countries: A Systematic Review and Meta-Analysis ((i-iv)) SO NUTRIENTS LA English DT Review ID NUTRITIONAL-STATUS; ZINC STATUS; VITAMIN-A; SCHOOLCHILDREN; GROWTH; IMPROVES; FORTIFICATION; SUPPLEMENTS; HEMOGLOBIN; COGNITION AB Multiple-micronutrient (MMN) fortification of beverages may be an effective option to deliver micronutrients to vulnerable populations. The aim of the present systematic review and meta-analysis is to evaluate the nutritional impacts of MMN fortified beverages in the context of low-middle income countries. A systematic search of published literature yielded 1022 citations, of which 10 randomized controlled trials (nine in school-aged children and one in pregnant women) met inclusion criteria. Results of school-aged children were included in the meta-analysis. Compared to iso-caloric controls, children who received MMN fortified beverages for 8 weeks to 6 months showed significant improvements in hemoglobin (+2.76 g/L, 95% CI [1.19, 4.33], p = 0.004; 8 studies) and serum ferritin (+15.42 pmol/L, [5.73, 25.12], p = 0.007; 8 studies); and reduced risk of anemia (RR 0.58 [0.29, 0.88], p = 0.005; 6 studies), iron deficiency (RR 0.34 [0.21, 0.55], p = 0.002; 7 studies), and iron deficiency anemia (RR 0.17 [0.06, 0.53], p = 0.02; 3 studies). MMN fortified beverage interventions could have major programmatic implications for reducing the burden of anemia and iron deficiency in school-aged children in low-middle income countries. Additional research is needed to investigate effects on other biochemical outcomes and population subgroups. C1 [Aaron, Grant J.] GAIN, CH-1202 Geneva, Switzerland. [Dror, Daphna K.] USDA, Western Human Nutr Res Ctr, Livermore, CA 95616 USA. [Yang, Zhenyu] CCDC, Natl Inst Nutr & Hlth, Key Lab Trace Element Nutr, Minist Hlth China, Beijing 100050, Peoples R China. RP Aaron, GJ (reprint author), GAIN, Rue Vermont 37-39, CH-1202 Geneva, Switzerland. EM gjaaron@gainhealth.org; daphnadror@gmail.com; yang.zhenyuid@gmail.com RI Mireku, Michael/D-5262-2016 FU Coca Cola Company FX Supported by The Coca Cola Company. The funding source played no role in the study design, analyses, or interpretation of results. The authors thank Steven A. Abrams (Baylor College of Medicine) and S.M. Ziauddin Hyder (World Bank) for reviewing the articles included in the review and pre-submission drafts of the manuscript; and Jan M. Peerson (University of California, Davis) for statistical consultation. NR 28 TC 2 Z9 2 U1 2 U2 9 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6643 J9 NUTRIENTS JI Nutrients PD MAY PY 2015 VL 7 IS 5 BP 3847 EP 3868 DI 10.3390/nu7053847 PG 22 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CK5CT UT WOS:000356240500045 PM 26007336 ER PT J AU Weise, DR Johnson, TJ Reardon, J AF Weise, David R. Johnson, Timothy J. Reardon, James TI Particulate and trace gas emissions from prescribed burns in southeastern US fuel types: Summary of a 5-year project SO FIRE SAFETY JOURNAL LA English DT Article DE Smoke; Pinus palustris; Pocosin; Spectroscopy; Wildland fire ID TRANSFORM INFRARED-SPECTROSCOPY; HYDROGEN-PEROXIDE H2O2; LONGLEAF PINE FORESTS; UNITED-STATES; FIRE BEHAVIOR; VIBRATIONAL ASSIGNMENTS; LABORATORY MEASUREMENTS; ATMOSPHERIC CHEMISTRY; SMOKE EXPOSURE; NORTH-CAROLINA AB Management of smoke from prescribed fires requires knowledge of fuel quantity and the amount and composition of the smoke produced by the fire to minimize adverse impacts on human health. A five-year study produced new emissions information for more than 100 trace gases and particulate matter in smoke for fuel types found in the southern United States of America using state-of-the-art instrumentation in both laboratory and field experiments. Emission factors for flaming, smoldering, and residual smoldering were developed. Agreement between laboratory and field-derived emission factors was generally good in most cases. Reference spectra of over 50 wildland fire gas-phase smoke components were added to a publicly-available database to support identification via infrared spectroscopy. Fuel loading for the field experiments was similar to previously measured fuels. This article summarizes the results of a five-year study to better understand the composition of smoke during all phases of burning for such forests. Published by Elsevier Ltd. C1 [Weise, David R.] US Forest Serv, USDA, Pacific Southwest Res Stn, Riverside, CA 92507 USA. [Johnson, Timothy J.] Pacific NW Natl Lab, Richland, WA 99352 USA. [Reardon, James] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59808 USA. RP Weise, DR (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM dweise@fs.fed.us; timothy.johnson@pnnl.gov; jreardon@fs.fed.us FU Department of Defense's Strategic Environmental Research and Development Program (SERDP) [RC-1649]; U.S. Department of Energy by Battelle Memorial Institute [DE-AC06-76RLO 1830] FX This work was supported by the Department of Defense's Strategic Environmental Research and Development Program (SERDP) project RC-1649 and we thank Dr. John Hall and his program for their support. Suggestions made by the SERDP Technical Advisory Committee helped to focus and expand the original project. In particular, we thank Prof. Robert J. Yokelson of the University of Montana for his many enormous contributions to this project. We also thank the several students, post-doctoral fellows and research scientists who contributed to individual components. In addition to SERDP and the Department of Defense installations, several other federal, state, and university organizations contributed to the success of this project including the National Oceanographic and Atmospheric Administration, the Environmental Protection Agency, the National Center for Atmospheric Research, Colorado State University, University of California-Riverside, Georgia Institute of Technology, North Carolina Forest Service, South Carolina Forestry Commission, the U.S. Forest Service, the Department of Energy, and the Pacific Northwest National Laboratory. PNNL is operated for the U.S. Department of Energy by the Battelle Memorial Institute under contract DE-AC06-76RLO 1830. NR 96 TC 1 Z9 1 U1 1 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0379-7112 EI 1873-7226 J9 FIRE SAFETY J JI Fire Saf. J. PD MAY PY 2015 VL 74 BP 71 EP 81 DI 10.1016/j.firesaf.2015.02.016 PG 11 WC Engineering, Civil; Materials Science, Multidisciplinary SC Engineering; Materials Science GA CK3HV UT WOS:000356108100008 ER PT J AU Kono, A Sato, A Reisch, B Cadle-Davidson, L AF Kono, Atsushi Sato, Akihiko Reisch, Bruce Cadle-Davidson, Lance TI Effect of Detergent on the Quantification of Grapevine Downy Mildew Sporangia from Leaf Discs SO HORTSCIENCE LA English DT Article DE Plasmopara viticola; disease resistance; Tween 20; adhesion; dried droplet; Vitis ID PATHOGEN PLASMOPARA-VITICOLA; FOLIAR RESISTANCE; ELSINOE-AMPELINA; LOCUS; INFECTION; GERMPLASM; MAP AB Grapevine downy mildew (DM), caused by the oomycete Plasmopara viticola (Berk. & Curt.) Berlese & de Toni, is a major disease, especially in humid viticultural areas. Development of resistant cultivars is an important objective for grapevine breeding. To establish a reliable and inexpensive quantitative method to aid in breeding for DM resistance, we improved the method for counting the number of sporangia on leaf discs, and developed a method for counting the number of sporangia in solution. To prevent the loss of DM sporangia from adhesion onto plastic ware, we found as little as 0.01% Tween 20 was effective. On the other hand, this detergent was shown to have a severe inhibitory effect upon DM infection a leaves. We developed a sporangia counting method using dried droplets of DM suspensions, and the method was highly correlated with counting by hemacytometer (R-2>0.96). The nonparametric Spearman's rank correlations between visual rating and the number of the sporangia were as high as rho = 0.82 to 0.91, suggesting that evaluation by the visual rating could provide a good estimate of the sporangia numbers on leaf discs. We established a high-throughput and inexpensive method with acceptable accuracy for DM resistance evaluation based on a leaf disc assay, and our results suggested that visual ratings of infected leaf discs provide a good estimate of sporangia numbers. C1 [Kono, Atsushi; Sato, Akihiko] NARO, Grape & Persimmon Res Stn, Natl Agr & Food Res Org Inst Fruit Tree Sci NIFTS, Higashihiroshima, Hiroshima 7392494, Japan. [Reisch, Bruce] Cornell Univ, New York State Agr Expt Stn, Hort Sect, Sch Integrat Plant Sci, Geneva, NY 14456 USA. [Cadle-Davidson, Lance] USDA, Grape Genet Res Unit, ARS, Geneva, NY 14456 USA. RP Kono, A (reprint author), NARO, Grape & Persimmon Res Stn, Natl Agr & Food Res Org Inst Fruit Tree Sci NIFTS, 301-2 Mitsu, Higashihiroshima, Hiroshima 7392494, Japan. EM akono@affrc.go.jp FU NARO Overseas Research Grant; USDA-National Institute of Food and Agriculture's VitisGen project [2011-51181-30635] FX We thank David Gadoury and Robert Seem for insightful discussions including the suggestion of the dried droplet method, and Steve Luce for technical assistance. This work was supported by a NARO Overseas Research Grant (long-term). Also, the grapevines were maintained with support from the USDA-National Institute of Food and Agriculture's VitisGen project (Award No. 2011-51181-30635). NR 28 TC 0 Z9 0 U1 6 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2015 VL 50 IS 5 BP 656 EP 660 PG 5 WC Horticulture SC Agriculture GA CJ8UA UT WOS:000355777800004 ER PT J AU Bell, RL AF Bell, Richard L. TI Effect of Resistant and Susceptible East European Pears on Development and Mortality Of the Pear Psylla, Cacopsylla pyricola (Forster) SO HORTSCIENCE LA English DT Article DE Pyrus; Sorbopyrus; Hemiptera; Psyllidae; antibiosis; antixenosis; feeding ID PYRUS GERMPLASM; HOMOPTERA PSYLLIDAE; BEHAVIOR; NYMPHS; CULTIVARS AB Pear psyllids (Cacopsylla spp., Hemiptera: Psyllidae) are major pests of European pear (Pyrus con:minis L.) in North America and Europe. In breeding resistant cultivars, a thorough understanding of the nature of host resistance and susceptibility is essential for designing efficient and accurate methods to identify resistant host genotypes. A developmental assay was performed on six genotypes previously identified as highly resistant, moderately resistant, or susceptible to nymphal feeding. Plants were infested with first instars of Cacopsylla pyricola (Forster), and feeding, mortality, and larval stage were observed for 34 days. Host genotypes significantly differed in feeding antixenosis at day 1 after infestation. Differences in antibiosis were also evident, as mortality of nymphs was greater on the feeding-resistant genotypes. There were also differences in the total numbers of surviving psylla and those developing to adults. Survival was directly related to the feeding antixenosis. There were no significant differences among the feeding-susceptible and moderately resistant genotypes in population age structure, except at day 19, reflecting the advanced stage of the few nymphs that did survive. Early feeding antixenosis was associated with mortality, either through a lack of feeding stimulation, feeding inhibition, or because of a direct toxic effect of the resistant plant genotypes. The short-term feeding antixenosis assay is predictive of long-term mortality and, to a lesser extent, with delayed development. 'Bartlett' and the wild seedling Y-167 were confirmed as susceptible, 'Karamanlika' and NY103-52 were confirmed as moderately resistant, and Bacui-1 and Bacui-2 were confirmed as highly resistant. C1 USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Bell, RL (reprint author), USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. EM richard.bell@ars.usda.gov NR 35 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2015 VL 50 IS 5 BP 661 EP 665 PG 5 WC Horticulture SC Agriculture GA CJ8UA UT WOS:000355777800005 ER PT J AU Ehlenfeldt, MK Vinyard, BT AF Ehlenfeldt, Mark K. Vinyard, Bryan T. TI Pruning Time X Cultivar Effects on Flower-bud Hardiness in Northern Highbush and Southern Highbush Blueberry SO HORTSCIENCE LA English DT Article DE Legacy; Jersey; LT50; Vaccinium darrowii; Vaccinium corymbosum ID COLD-HARDINESS; DEACCLIMATION; GENOTYPES AB A study was conducted to determine if early-fall pruning (vs. more typical winter pruning) of either northern highbush or southern highbush blueberries was detrimental to the development of optimum levels of mid-winter cold hardiness in floral buds under New Jersey conditions. Using a detached-shoot freeze-thaw assay, flower-bud LT50 values were determined in early January for both 'Jersey' (northern highbush) and 'Legacy' (southern highbush) blueberry bushes that had been subjected to early- or late-pruning protocols. Across 2 years, intrinsic differences due to genotype and genotype x year were present, but no significant differences due to pruning time were observed. The lack of pruning effects on flower-bud LT50 values suggests that cultivars with southern germplasm selected in areas such as North Carolina and further north may be treated similarly to northern highbush with respect to pruning time, including early-fall pruning. C1 [Ehlenfeldt, Mark K.] USDA ARS, PE Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. [Vinyard, Bryan T.] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. RP Ehlenfeldt, MK (reprint author), USDA ARS, PE Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. EM mark.ehlenfeldt@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2015 VL 50 IS 5 BP 673 EP 675 PG 3 WC Horticulture SC Agriculture GA CJ8UA UT WOS:000355777800007 ER PT J AU Schmelcher, M Shen, Y Nelson, DC Eugster, MR Eichenseher, F Hanke, DC Loessner, MJ Dong, SL Pritchard, DG Lee, JC Becker, SC Foster-Frey, J Donovan, DM AF Schmelcher, Mathias Shen, Yang Nelson, Daniel C. Eugster, Marcel R. Eichenseher, Fritz Hanke, Daniela C. Loessner, Martin J. Dong, Shengli Pritchard, David G. Lee, Jean C. Becker, Stephen C. Foster-Frey, Juli Donovan, David M. TI Evolutionarily distinct bacteriophage endolysins featuring conserved peptidoglycan cleavage sites protect mice from MRSA infection SO JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY LA English DT Article DE peptidoglycan hydrolase; antimicrobial; biofilm; antibiotic resistance ID RESISTANT STAPHYLOCOCCUS-AUREUS; WALL TEICHOIC-ACID; BACTERIOLYTIC AGENT; NASAL COLONIZATION; LYTIC ACTIVITY; UNITED-STATES; RESCUES MICE; LYSOSTAPHIN; DOMAIN; LYSIS AB Objectives: In the light of increasing drug resistance in Staphylococcus aureus, bacteriophage endolysins [peptidoglycan hydrolases (PGHs)] have been suggested as promising antimicrobial agents. The aim of this study was to determine the antimicrobial activity of nine enzymes representing unique homology groups within a diverse class of staphylococcal PGHs. Methods: PGHs were recombinantly expressed, purified and tested for staphylolytic activity in multiple in vitro assays (zymogram, turbidity reduction assay and plate lysis) and against a comprehensive set of strains (S. aureus and CoNS). PGH cut sites in the staphylococcal peptidoglycan were determined by biochemical assays (Park-Johnson and Ghuysen procedures) and MS analysis. The enzymes were tested for their ability to eradicate static S. aureus biofilms and compared for their efficacy against systemic MRSA infection in a mouse model. Results: Despite similar modular architectures and unexpectedly conserved cleavage sites in the peptidoglycan (conferred by evolutionarily divergent catalytic domains), the enzymes displayed varying degrees of in vitro lytic activity against numerous staphylococcal strains, including cell surface mutants and drug-resistant strains, and proved effective against static biofilms. In a mouse model of systemic MRSA infection, six PGHs provided 100% protection from death, with animals being free of clinical signs at the end of the experiment. Conclusions: Our results corroborate the high potential of PGHs for treatment of S. aureus infections and reveal unique antimicrobial and biochemical properties of the different enzymes, suggesting a high diversity of potential applications despite highly conserved peptidoglycan target sites. C1 [Schmelcher, Mathias; Becker, Stephen C.; Foster-Frey, Juli; Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, ANRI, NEA,USDA, Beltsville, MD 20705 USA. [Schmelcher, Mathias; Shen, Yang; Eugster, Marcel R.; Eichenseher, Fritz; Hanke, Daniela C.; Loessner, Martin J.] ETH, Inst Food Nutr & Hlth, CH-8092 Zurich, Switzerland. [Shen, Yang; Nelson, Daniel C.] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD 20850 USA. [Nelson, Daniel C.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. [Dong, Shengli; Pritchard, David G.] Univ Alabama Birmingham, Dept Biochem & Mol Genet, MCLM 552, Birmingham, AL 35294 USA. [Lee, Jean C.] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA 02115 USA. [Lee, Jean C.] Harvard Univ, Sch Med, Boston, MA 02115 USA. RP Schmelcher, M (reprint author), ETH, Inst Food Nutr & Hlth, Schmelzbergstr 7, CH-8092 Zurich, Switzerland. EM mathias.schmelcher@hest.ethz.ch RI Loessner, Martin/B-4316-2012 FU National Institutes of Health [1RO1AI075077-01A1]; National Research Initiative [2007-35204-18395]; United States State Department funds FX This work was supported by the National Institutes of Health (grant number 1RO1AI075077-01A1 to D. M. D. and J. C. L.), the National Research Initiative (grant number 2007-35204-18395 to D. M. D.) and United States State Department funds supporting a US-Pakistani (to D. M. D.) and US-Russian collaboration (to D. M. D.). NR 63 TC 11 Z9 13 U1 1 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7453 EI 1460-2091 J9 J ANTIMICROB CHEMOTH JI J. Antimicrob. Chemother. PD MAY PY 2015 VL 70 IS 5 BP 1453 EP 1465 DI 10.1093/jac/dku552 PG 13 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA CK0YW UT WOS:000355932800023 PM 25630640 ER PT J AU Roze, LV Laivenieks, M Hong, SY Wee, J Wong, SS Vanos, B Awad, D Ehrlich, KC Linz, JE AF Roze, Ludmila V. Laivenieks, Maris Hong, Sung-Yong Wee, Josephine Wong, Shu-Shyan Vanos, Benjamin Awad, Deena Ehrlich, Kenneth C. Linz, John E. TI Aflatoxin Biosynthesis Is a Novel Source of Reactive Oxygen Species-A Potential Redox Signal to Initiate Resistance to Oxidative Stress? SO TOXINS LA English DT Article ID FUNGAL SECONDARY METABOLISM; ASPERGILLUS-PARASITICUS; NADPH OXIDASES; PODOSPORA-ANSERINA; GENE-CLUSTER; CYTOCHROME-P450; GENERATION; ENZYMES; FLAVUS; DIFFERENTIATION AB Aflatoxin biosynthesis in the filamentous fungus Aspergillus parasiticus involves a minimum of 21 enzymes, encoded by genes located in a 70 kb gene cluster. For aflatoxin biosynthesis to be completed, the required enzymes must be transported to specialized early and late endosomes called aflatoxisomes. Of particular significance, seven aflatoxin biosynthetic enzymes are P450/monooxygenases which catalyze reactions that can produce reactive oxygen species (ROS) as byproducts. Thus, oxidative reactions in the aflatoxin biosynthetic pathway could potentially be an additional source of intracellular ROS. The present work explores the hypothesis that the aflatoxin biosynthetic pathway generates ROS (designated as "secondary" ROS) in endosomes and that secondary ROS possess a signaling function. We used specific dyes that stain ROS in live cells and demonstrated that intracellular ROS levels correlate with the levels of aflatoxin synthesized. Moreover, feeding protoplasts with precursors of aflatoxin resulted in the increase in ROS generation. These data support the hypothesis. Our findings also suggest that secondary ROS may fulfill, at least in part, an important mechanistic role in increased tolerance to oxidative stress in germinating spores (seven-hour germlings) and in regulation of fungal development. C1 [Roze, Ludmila V.; Hong, Sung-Yong; Wee, Josephine; Wong, Shu-Shyan; Vanos, Benjamin; Awad, Deena; Linz, John E.] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. [Roze, Ludmila V.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Laivenieks, Maris; Linz, John E.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Ehrlich, Kenneth C.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Linz, John E.] Michigan State Univ, Ctr Integrat Toxicol, E Lansing, MI 48824 USA. RP Linz, JE (reprint author), Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. EM roze@msu.edu; laivenie@msu.edu; lunohong@yahoo.co.kr; weejosep@msu.edu; wongs94@yahoo.com; vanosben@msu.edu; awaddeen@msu.edu; ehrlich8@gmail.com; jlinz@msu.edu FU NIH [CA 52003-21]; Michigan State University AgBio Research FX This work was supported by NIH grant CA 52003-21 (J.E.L.) and Michigan State University AgBio Research. NR 57 TC 7 Z9 7 U1 4 U2 17 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD MAY PY 2015 VL 7 IS 5 BP 1411 EP 1430 DI 10.3390/toxins7051411 PG 20 WC Toxicology SC Toxicology GA CK5CU UT WOS:000356240600002 PM 25928133 ER PT J AU Kerr, KL Meinzer, FC McCulloh, KA Woodruff, DR Marias, DE AF Kerr, Kelly L. Meinzer, Frederick C. McCulloh, Katherine A. Woodruff, David R. Marias, Danielle E. TI Expression of functional traits during seedling establishment in two populations of Pinus ponderosa from contrasting climates SO TREE PHYSIOLOGY LA English DT Article DE carbon isotope discrimination; gas exchange; genetic variation; hydraulic architecture; osmotic potential; phenology ID WATER-USE EFFICIENCY; CARBON-ISOTOPE DISCRIMINATION; FIR PSEUDOTSUGA-MENZIESII; DOUGLAS-FIR; GAS-EXCHANGE; GENETIC DIFFERENTIATION; HYDRAULIC CONDUCTANCE; XYLEM CAVITATION; BETULA-OCCIDENTALIS; BIOMASS ALLOCATION AB First-year tree seedlings represent a particularly vulnerable life stage and successful seedling establishment is crucial for forest regeneration. We investigated the extent to which Pinus ponderosa P. & C. Lawson populations from different climate zones exhibit differential expression of functional traits that may facilitate their establishment. Seeds from two populations from sites with contrasting precipitation and temperature regimes east (PIPOdry) and west (PIPOmesic) of the Oregon Cascade mountains were sown in a common garden experiment and grown under two water availability treatments (control and drought). Aboveground biomass accumulation, vegetative phenology, xylem anatomy, plant hydraulic architecture, foliar stable carbon isotope ratios (delta C-13), gas exchange and leaf water relations characteristics were measured. No treatment or population-related differences in leaf water potential were detected. At the end of the first growing season, aboveground biomass was 74 and 44% greater in PIPOmesic in the control and drought treatments, respectively. By early October, 73% of PIPOdry seedlings had formed dormant buds compared with only 15% of PIPOmesic seedlings. Stem theoretical specific conductivity, calculated from tracheid dimensions and packing density, declined from June through September and was nearly twice as high in PIPOmesic during most of the growing season, consistent with measured values of specific conductivity. Intrinsic water-use efficiency based on delta C-13 values was higher in PIPOdry seedlings for both treatments across all sampling dates. There was a negative relationship between values of delta C-13 and leaf-specific hydraulic conductivity across populations and treatments, consistent with greater stomatal constraints on gas exchange with declining seedling hydraulic capacity. Integrated growing season assimilation and stomatal conductance estimated from foliar delta C-13 values and photosynthetic CO2-response curves were 6 and 28% lower, respectively, in PIPOdry seedlings. Leaf water potential at the turgor loss point was 0.33 MPa more negative in PIPOdry, independent of treatment. Overall, PIPOdry seedlings exhibited more conservative behavior, suggesting reduced growth is traded off for increased resistance to drought and extreme temperatures. C1 [Kerr, Kelly L.; Marias, Danielle E.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Meinzer, Frederick C.; Woodruff, David R.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [McCulloh, Katherine A.] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. RP Meinzer, FC (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM rick.meinzer@oregonstate.edu FU National Science Foundation [IOS-1146751] FX This project was funded by the National Science Foundation (IOS-1146751). NR 85 TC 3 Z9 3 U1 8 U2 34 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD MAY PY 2015 VL 35 IS 5 BP 535 EP 548 DI 10.1093/treephys/tpv034 PG 14 WC Forestry SC Forestry GA CK2DP UT WOS:000356021100007 PM 25934987 ER PT J AU Minocha, R Chamberlain, B Long, S Turlapati, SA Quigley, G AF Minocha, Rakesh Chamberlain, Bradley Long, Stephanie Turlapati, Swathi A. Quigley, Gloria TI Extraction and estimation of the quantity of calcium oxalate crystals in the foliage of conifer and hardwood trees SO TREE PHYSIOLOGY LA English DT Article DE aspen; beech; birch; conifers; fir; hardwoods; hemlock; HPLC; maple; oak; organic acids; pine; spruce ID GENE-EXPRESSION; ORGANIC-ACIDS; LEAVES; PLANTS; DEPLETION; TISSUE; SOILS AB The main goal of this study was to develop a method for the extraction and indirect estimation of the quantity of calcium oxalate (CaOx) in the foliage of trees. Foliar tissue was collected from a single tree of each species (five conifers and five hardwoods) for comparison of extractions in different solvents using 10 replicates per species from the same pool of tissue. For each species, calcium (Ca) and oxalate were extracted sequentially in double deionized water and 2N acetic acid, and finally, five replicate samples were extracted in 5% (0.83N) perchloric acid (PCA) and the other five in 2N hydrochloric acid (HCl); three cycles of freezing and thawing were used for each solvent. Total ions were extracted by microwave digestion. Calcium was quantified with an inductively coupled plasma emission spectrophotometer method and oxalate was eluted and quantified using a high performance liquid chromatography method. This experiment was repeated again with two conifer and two hardwood species using four trees per species, and two analytical replicates for each tree. We report here that, regardless of age of individual trees within a species, time of collection or species type, the third extraction in PCA or HCl resulted in near equimolar quantities of Ca and oxalate (r(2) >= 0.99). This method provides an easy estimate of the quantity of CaOx crystals using a small sample of foliar tissue. An additional benefit of PCA is that it precipitates the nucleic acids and proteins, allowing the quantification of several free/soluble metabolites such as amino acids, polyamines, organic acids and inorganic elements all from a single sample extract. C1 [Minocha, Rakesh; Chamberlain, Bradley; Long, Stephanie; Turlapati, Swathi A.; Quigley, Gloria] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Turlapati, Swathi A.; Quigley, Gloria] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. RP Minocha, R (reprint author), US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. EM rminocha@fs.fed.us FU USDA Forest Service, Northern Research Station FX This work was fully supported by funding received from USDA Forest Service, Northern Research Station. NR 27 TC 0 Z9 0 U1 3 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD MAY PY 2015 VL 35 IS 5 BP 574 EP 580 DI 10.1093/treephys/tpv031 PG 7 WC Forestry SC Forestry GA CK2DP UT WOS:000356021100010 PM 25934989 ER PT J AU Benzaquen, M Galvao, KN Coleman, AE Santos, JEP Goff, JP Risco, CA AF Benzaquen, M. Galvao, K. N. Coleman, A. E. Santos, J. E. P. Goff, J. P. Risco, C. A. TI Effect of oral mineral and energy supplementation on blood mineral concentrations, energetic and inflammatory profile, and milk yield in dairy cows affected with dystocia SO VETERINARY JOURNAL LA English DT Article DE Subclinical hypocalcemia; Calcium supplementation; Energy supplementation; Dairy cows ID BODY CONDITION SCORE; DRY-MATTER INTAKE; HOLSTEIN COWS; NEUTROPHIL FUNCTION; EARLY-LACTATION; SUBCLINICAL HYPOCALCEMIA; PROPYLENE-GLYCOL; CALCIUM-CHLORIDE; VITAMIN-D; DISORDERS AB The objectives of this study were to determine the effect of mineral/energy supplementation of dairy cows with dystocia on blood mineral concentrations, energetic and inflammatory profiles, and milk yield. Multiparous Holstein cows with dystocia were randomly assigned into two groups, (1) treated with a mineral/energy supplement (DME, n =18) and (2) not treated (DNT, n =22). A group of cows with normal parturition were randomly selected and were left untreated (NNT, n =25). Cows in DME received an oral drench of 110 g of calcium and 400 g of propionate as calcium propionate plus 110 g potassium chloride and 150 g of magnesium sulfate administered within 6 h of calving and again 3 days post-partum. Compared to cows with a normal parturition, dystocic cows had decreased plasma calcium concentrations, increased plasma haptoglobin, decreased milk yield at 1 day post-partum, and tended to have increased rectal temperatures from 1 to 12 days post-partum. Compared with cows in DNT, those in DME had decreased plasma calcium concentrations and increased plasma magnesium concentrations 2 and 3 days post-partum, and a tendency for an increase in rectal temperature from 1 to 12 days postpartum. Dystocia is detrimental to calcium homeostasis post-partum, but mineral/energy supplementation as undertaken in this study is not recommended for use in cows with dystocia. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Benzaquen, M.; Galvao, K. N.; Coleman, A. E.; Risco, C. A.] Univ Florida, Dept Large Anim Clin Sci, Gainesville, FL 32610 USA. [Galvao, K. N.] Univ Florida, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32610 USA. [Santos, J. E. P.] Univ Florida, Dept Anim Sci, Gainesville, FL 32610 USA. [Goff, J. P.] USDA ARS, Periparturient Dis Cattle Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Galvao, KN (reprint author), Univ Florida, Dept Large Anim Clin Sci, Gainesville, FL 32610 USA. EM galvaok@ufl.edu RI Santos, Jose/K-5564-2013 OI Santos, Jose/0000-0003-3403-1465 NR 39 TC 1 Z9 1 U1 1 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1090-0233 EI 1532-2971 J9 VET J JI Vet. J. PD MAY PY 2015 VL 204 IS 2 BP 186 EP 191 DI 10.1016/j.tvjl.2015.03.001 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA CK3JP UT WOS:000356112700011 PM 25900193 ER PT J AU Thairu, MW Brunet, J AF Thairu, Margaret W. Brunet, Johanne TI The role of pollinators in maintaining variation in flower colour in the Rocky Mountain columbine, Aquilegia coerulea SO ANNALS OF BOTANY LA English DT Article DE Aquilegia coerulea; Ranunculaceae; Rocky Mountain columbine; flower colour variation; pollinators; bumble-bees; Bombus impatiens; hawkmoths; Hyles lineata; learning behaviour; novelty; frequency dependence ID FREQUENCY-DEPENDENT SELECTION; BUMBLEBEE BOMBUS-TERRESTRIS; MEDIATED SELECTION; MANDUCA-SEXTA; CAERULEA RANUNCULACEAE; NOCTURNAL HAWKMOTHS; FORAGING BEHAVIOR; EVOLUTION; VISION; PREFERENCES AB Background and Aims Flower colour varies within and among populations of the Rocky Mountain columbine, Aquilegia coerulea, in conjunction with the abundance of its two major pollinators, hawkmoths and bumble-bees. This study seeks to understand whether the choice of flower colour by these major pollinators can help explain the variation in flower colour observed in A. coerulea populations. Methods Dual choice assays and experimental arrays of blue and white flowers were used to determine the preference of hawkmoths and bumble-bees for flower colour. A test was made to determine whether a differential preference for flower colour, with bumble-bees preferring blue and hawkmoths white flowers, could explain the variation in flower colour. Whether a single pollinator could maintain a flower colour polymorphism was examined by testing to see if preference for a flower colour varied between day and dusk for hawkmoths and whether bumble-bees preferred novel or rare flower colour morphs. Key Results Hawkmoths preferred blue flowers under both day and dusk light conditions. Naive bumble-bees preferred blue flowers but quickly learned to forage randomly on the two colour morphs when similar rewards were presented in the flowers. Bees quickly learned to associate a flower colour with a pollen reward. Prior experience affected the choice of flower colour by bees, but they did not preferentially visit novel flower colours or rare or common colour morphs. Conclusions Differences in flower colour preference between the two major pollinators could not explain the variation in flower colour observed in A. coerulea. The preference of hawkmoths for flower colour did not change between day and dusk, and bumble-bees did not prefer a novel or a rare flower colour morph. The data therefore suggest that factors other than pollinators may be more likely to affect the flower colour variation observed in A. coerulea. C1 [Thairu, Margaret W.; Brunet, Johanne] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Brunet, Johanne] ARS, USDA, Vegetable Crop Res Unit, Madison, WI 53706 USA. RP Brunet, J (reprint author), Univ Wisconsin, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM jbrunet@wisc.edu FU Graduate Women in Science SDE/GWIS chapter at the University of Wisconsin in Madison; Department of Agriculture, Agricultural Research Service FX We thank Joshua Kluever, Christy Stewart, Megan Van Etten and Ross Ziobro for their help with data collection, and Dr Murray Clayton for his statistical advice. This work was supported by a Science and Medicine Graduate Research Scholars Fellowship (SciMed GRS) and a Ruth Dickie Award from the Graduate Women in Science SDE/GWIS chapter at the University of Wisconsin in Madison to M.W.T., and from funds from the Department of Agriculture, Agricultural Research Service, to J.B. NR 60 TC 1 Z9 1 U1 8 U2 38 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7364 EI 1095-8290 J9 ANN BOT-LONDON JI Ann. Bot. PD MAY PY 2015 VL 115 IS 6 BP 971 EP 979 DI 10.1093/aob/mcv028 PG 9 WC Plant Sciences SC Plant Sciences GA CJ6SX UT WOS:000355625000012 PM 25808657 ER PT J AU Wesley-Smith, J Walters, C Pammenter, NW Berjak, P AF Wesley-Smith, James Walters, Christina Pammenter, N. W. Berjak, Patricia TI Why is intracellular ice lethal? A microscopical study showing evidence of programmed cell death in cryo-exposed embryonic axes of recalcitrant seeds of Acer saccharinum SO ANNALS OF BOTANY LA English DT Article DE Acer saccharinum; Aceraceae; autophagy; cooling rate; cryopreservation; embryonic axes; intracellular ice; light microscopy; mechanical stress; programmed cell death; recalcitrant seed; silver maple; transmission electron microscopy; TEM ID PONCIRUS-TRIFOLIATA L.; SHOOT TIPS; DIRECTIONAL SOLIDIFICATION; MECHANICAL COMPRESSION; DESICCATION TOLERANCE; ELECTRON MICROSCOPY; WATER CONTENTS; CRYOPRESERVATION; GERMPLASM; SURVIVAL AB Background and Aims Conservation of the genetic diversity afforded by recalcitrant seeds is achieved by cryopreservation, in which excised embryonic axes (or, where possible, embryos) are treated and stored at temperatures lower than -180 degrees C using liquid nitrogen. It has previously been shown that intracellular ice forms in rapidly cooled embryonic axes of Acer saccharinum (silver maple) but this is not necessarily lethal when ice crystals are small. This study seeks to understand the nature and extent of damage from intracellular ice, and the course of recovery and regrowth in surviving tissues. Methods Embryonic axes of A. saccharinum, not subjected to dehydration or cryoprotection treatments (water content was 1.9g H2O g(-1) dry mass), were cooled to liquid nitrogen temperatures using two methods: plunging into nitrogen slush to achieve a cooling rate of 97 degrees Cs-1 or programmed cooling at 3.3 degrees Cs-1. Samples were thawed rapidly (177 degrees Cs-1) and cell structure was examined microscopically immediately, and at intervals up to 72 h in vitro. Survival was assessed after 4 weeks in vitro. Axes were processed conventionally for optical microscopy and ultrastructural examination. Key Results Immediately following thaw after cryogenic exposure, cells from axes did not show signs of damage at an ultrastructural level. Signs that cells had been damaged were apparent after several hours of in vitro culture and appeared as autophagic decomposition. In surviving tissues, dead cells were sloughed off and pockets of living cells were the origin of regrowth. In roots, regrowth occurred from the ground meristem and procambium, not the distal meristem, which became lethally damaged. Regrowth of shoots occurred from isolated pockets of surviving cells of peripheral and pith meristems. The size of these pockets may determine the possibility for, the extent of and the vigour of regrowth. Conclusions Autophagic degradation and ultimately autolysis of cells following cryo-exposure and formation of small (0.2-0.4 mu m) intracellular ice crystals challenges current ideas that ice causes immediate physical damage to cells. Instead, freezing stress may induce a signal for programmed cell death (PCD). Cells that form more ice crystals during cooling have faster PCD responses. C1 [Wesley-Smith, James; Pammenter, N. W.; Berjak, Patricia] Univ KwaZulu Natal, Sch Life Sci, Plant Germplasm Conservat Res, ZA-4001 Durban, South Africa. [Wesley-Smith, James] CSIR, Natl Ctr Nanostruct Mat, ZA-0002 Pretoria, South Africa. [Walters, Christina] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Walters, C (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80521 USA. EM Christina.Walters@ARS.USDA.GOV FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS); National Research Foundation (South Africa) FX This work was supported by funding from the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) and the National Research Foundation (South Africa). We thank Lisa Hill and Jennifer Crane for expert technical assistance. We acknowledge the insightful and knowledgeable comments made by the anonymous reviewers. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 42 TC 1 Z9 2 U1 18 U2 37 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7364 EI 1095-8290 J9 ANN BOT-LONDON JI Ann. Bot. PD MAY PY 2015 VL 115 IS 6 BP 991 EP 1000 DI 10.1093/aob/mcv009 PG 10 WC Plant Sciences SC Plant Sciences GA CJ6SX UT WOS:000355625000014 PM 25808653 ER PT J AU Soong, JL Parton, WJ Calderon, F Campbell, EE Cotrufo, MF AF Soong, Jennifer L. Parton, William J. Calderon, Francisco Campbell, Eleanor E. Cotrufo, M. Francesca TI A new conceptual model on the fate and controls of fresh and pyrolized plant litter decomposition SO BIOGEOCHEMISTRY LA English DT Article DE Leaf litter; Pyrogenic organic matter; Decomposition; Dissolved organic matter; Dissolved organic carbon; Leaching; Respiration; FTIR ID DISSOLVED ORGANIC-MATTER; CARBON-USE EFFICIENCY; ACID-DETERGENT FIBER; CLIMATE-CHANGE; LEAF-LITTER; SOIL RESPIRATION; TEMPERATE FOREST; MICROBIAL BIOMASS; DECIDUOUS FOREST; FOLIAR LITTER AB In recent years, litter decomposition studies have begun to move beyond the concept of mass loss to consider the fate of fresh and pyrolized decomposing plant material in the ecosystem. However, these concepts have yet to be incorporated into conceptual models of litter decomposition. Understanding how fresh and pyrolized plant litter chemical traits control the partitioning of mass loss to dissolved organic carbon (DOC) leaching and respiration to CO2 would help to inform models of litter-soil-atmosphere carbon (C) cycling. To test these controls, we incubated five fresh and one pyrolized leaf litters with differing chemistry and measured DOC and CO2 fluxes as well as changes in substrate and dissolved organic matter (DOM) chemistry over time using Fourier transformed infrared spectroscopy and wet chemistry. We found that the amount of hot water extractable C was a strong predictor of initial DOC leaching, while the lignocellulose index [Lignin/(Lignin + alpha-Cellulose)] was a strong inverse predictor of later stage DOC:CO2 partitioning. Changes in substrate and DOM chemistry indicated a progression of substrate availability for leaching: from soluble plant components, to partially decomposed cellulose and lignin, to microbial products. Based on these results we developed a new conceptual model that demonstrates how chemical traits of fresh and pyrolyzed plant litter can be used to predict the fate of aboveground organic matter decomposition and form a better linkage between aboveground decomposition and terrestrial ecosystem C cycling. C1 [Soong, Jennifer L.; Parton, William J.; Campbell, Eleanor E.; Cotrufo, M. Francesca] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Calderon, Francisco] USDA ARS, Cent Great Plains Res Stn, Akron, OH USA. [Cotrufo, M. Francesca] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. RP Soong, JL (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. EM Jennifer.soong@colostate.edu RI Cotrufo, M. Francesca/C-1614-2013 OI Cotrufo, M. Francesca/0000-0002-6191-8953 FU NSF-DEB [0918482]; NSF Graduate Research Fellowship Program; NSF Research Experience for Teacher program FX This work would not have been possible without help from D. Rutherford at USGS, C. Rhodes and D. Pierson at USFS, K. Guilbert, D. Reuss, C. Pinney, J. Botte, and M. Jurich at Ecocore. The work was funded by the NSF-DEB grant #0918482, the NSF Graduate Research Fellowship Program and the NSF Research Experience for Teacher program. The analytical work was carried out at the EcoCore analytical services facility at Colorado State University (http://ecocore.nrel.colostate.edu/). NR 67 TC 13 Z9 14 U1 14 U2 92 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAY PY 2015 VL 124 IS 1-3 BP 27 EP 44 DI 10.1007/s10533-015-0079-2 PG 18 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CJ6RE UT WOS:000355620200003 ER PT J AU Stewart, CE Moturi, P Follett, RF Halvorson, AD AF Stewart, Catherine E. Moturi, Pratibha Follett, Ronald F. Halvorson, Ardell D. TI Lignin biochemistry and soil N determine crop residue decomposition and soil priming SO BIOGEOCHEMISTRY LA English DT Article DE Decomposition; Lignin; Temperature; Nitrogen; Dissolved organic carbon; Lignin biomarkers ID ORGANIC-MATTER DECOMPOSITION; PLANT LITTER DECOMPOSITION; LONG-TERM INCUBATION; CENTRAL GREAT-PLAINS; CARBON SEQUESTRATION; NITROGEN AVAILABILITY; MICROBIAL COMMUNITY; DYNAMICS; QUALITY; C-13 AB Residue lignin content and biochemistry are important properties influencing residue decomposition dynamics and native soil C loss through priming. The relative contribution of high lignin residues to soil organic matter (SOM) may be less than previously believed, be more sensitive to soil N status, and may be more sensitive to increased temperature. We examined the role of residue biochemistry, temperature, and soil N on the decomposition dynamics of five crop residues varying in lignin content and composition (corn, sorghum, soybean, sunflower and wheat). We used natural abundance delta(CO2)-C-13 to quantify residue decomposition and soil priming from a soil previously cropped to wheat-fallow or to corn-millet-wheat at 20 and 30 A degrees C in a laboratory incubation. High lignin residues decomposed more completely than low lignin residues, supporting a new model of SOM formation suggesting high lignin residues have a lower efficiency for stabilizing SOM due to inefficient microbial processing. However, residues with lower residue respiration had greater soil C respiration (soil priming). Residue SG lignin was positively related to residue C respired and H-lignin positively related to soil C respired in all soils and temperatures, resulting in no net lignin chemistry effect on the combined total C respired. Effects of lignin on residue decomposition were most apparent in treatments with lower soil N contents indicating N limitation. Measuring both residue and soil respiration and considering soil N status is important to accurately assess the effects of residue biochemistry on soil organic carbon. C1 [Stewart, Catherine E.; Follett, Ronald F.; Halvorson, Ardell D.] USDA ARS, Ft Collins, CO 80526 USA. [Moturi, Pratibha] Cent Res Inst Dryland Agr, Hyderabad 500059, Andhra Pradesh, India. RP Stewart, CE (reprint author), USDA ARS, 2150 Ctr Ave,Bldg D,Suite 100, Ft Collins, CO 80526 USA. EM catherine.stewart@colostate.edu OI Stewart, Catherine/0000-0003-1216-0450 FU Agricultural Research Service under the ARS GRACEnet Project FX The authors thank Elizabeth Pruessner, Erin Grogan, Mary Smith, Brad Floyd, Robert D'Adamo, Travis Delorean, and many students for their assistance and analytical support in collecting, processing, and analyzing the soil and plant samples and data reported herein and the Central Great Plains Research Staff for maintaining these long-term plots. The authors thank anonymous reviewers and the editor for their thoughtful comments and suggestions to improve the manuscript. This publication is based upon work supported by the Agricultural Research Service under the ARS GRACEnet Project. NR 69 TC 7 Z9 7 U1 8 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAY PY 2015 VL 124 IS 1-3 BP 335 EP 351 DI 10.1007/s10533-015-0101-8 PG 17 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CJ6RE UT WOS:000355620200022 ER PT J AU Akin, M Niedz, RP Reed, BM AF Akin, Meleksen Niedz, Randall P. Reed, Barbara M. TI Developing Improved Micropropagation Medium for Hazelnuts (Corylus avellana L.) Based on Ion Concentrations. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Akin, Meleksen] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Niedz, Randall P.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Reed, Barbara M.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM akinm@onid.oregonstate.edu NR 0 TC 0 Z9 0 U1 2 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2035 BP S60 EP S61 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000145 ER PT J AU Aldemir, G Kaya, E Yilmaz-Gokdogan, E Souza, FVD Jenderek, MM AF Aldemir, G. Kaya, E. Yilmaz-Gokdogan, E. Souza, F. V. D. Jenderek, M. M. TI Cryopreservation of Citrus Seeds Via Dehydration and Direct Immersion in Liquid Nitrogen. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Aldemir, G.; Yilmaz-Gokdogan, E.] Mugla Sitki Kocman Univ, Fac Sci, Dept Biol, TR-48000 Kotekli, Mugla, Turkey. [Kaya, E.] Mugla Sitki Kocman Univ, Fac Sci, Mol Biol & Genet Dept, TR-48000 Kotekli, Mugla, Turkey. [Souza, F. V. D.] Embrapa Cassava & Fruits, BR-44380000 Cruz Das Almas, BA, Brazil. [Jenderek, M. M.] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80526 USA. EM ergunkaya@mu.edu.tr NR 0 TC 0 Z9 0 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2018 BP S55 EP S55 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000128 ER PT J AU Jenderek, MM Reed, BM AF Jenderek, M. M. Reed, B. M. TI Cryobanking of Plant Species, Status and Promise SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Jenderek, M. M.] USDA ARS, Plant & Anim Genet Resources Preservat, NCGRP, Ft Collins, CO 80521 USA. [Reed, B. M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM maria.jenderek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-32 BP S24 EP S24 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000057 ER PT J AU Kamo, K Vieira, P Wantoch, S Lilley, C Chitwood, D Atkinson, H AF Kamo, K. Vieira, P. Wantoch, S. Lilley, C. Chitwood, D. Atkinson, H. TI Expression of a Cystatin Transgene Can Confer Resistance to Root Lesion Nematodes in Lilium longiflorum cv. 'Nellie White'. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kamo, K.; Vieira, P.; Wantoch, S.] USDA, Floral & Nursery Plants Res Unit, US Natl Arboretum, Beltsville, MD 20705 USA. [Lilley, C.; Atkinson, H.] Univ Leeds, Ctr Plant Sci, Leeds, W Yorkshire, England. [Chitwood, D.] USDA, Nematol Lab, Beltsville, MD 20705 USA. EM Kathryn.Kamo@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2032 BP S60 EP S60 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000142 ER PT J AU Lee, J Pijut, PM AF Lee, J. Pijut, P. M. TI Isolation and Functional Characterization of an AGAMOUS Homolog from Black Ash (Fraxinus nigra). SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Lee, J.] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr HTIRC, W Lafayette, IN 47907 USA. [Pijut, P. M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM lee1512@purdue.edu; ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2015 BP S54 EP S54 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000125 ER PT J AU Neugebauer, K Bruce, M Trick, H Fellers, J AF Neugebauer, Kerri Bruce, Myron Trick, Harold Fellers, John TI Wheat Gene Expression Differences Induced by Six Races of Puccinia triticina SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Neugebauer, Kerri; Trick, Harold] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Bruce, Myron; Fellers, John] USDA ARS GMPRC, Manhattan, KS 66506 USA. EM kerrin@ksu.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-1003 BP S32 EP S32 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000075 ER PT J AU Niedz, RP AF Niedz, R. P. TI ARS-MEDIA for Excel - An Excel Spreadsheet for the Calculation of Media Recipes Based on Ion-specific Constraints. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Niedz, R. P.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM randall.niedz@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2000 BP S48 EP S48 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000110 ER PT J AU Niedz, RP AF Niedz, Randall P. TI Design of Experiments (DOE) and In Vitro Culture - History, Concepts, and Relevance SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Niedz, Randall P.] USDA ARS, US Hort Res Lab, Ft Pierce, FL USA. EM Randall.Niedz@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-25 BP S21 EP S21 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000050 ER PT J AU Pence, VC Finke, L Niedz, R AF Pence, Valerie C. Finke, Linda Niedz, Randall TI Using a DOE Approach for Improving Growth of the Endangered Cycladenia humilis var. jonesii (Apocynaceae) In Vitro SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pence, Valerie C.; Finke, Linda] Cincinnati Zoo & Bot Garden, Ctr Conservat & Res Endangered Wildlife, Cincinnati, OH 45220 USA. [Niedz, Randall] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM valerie.pence@cincinnatizoo.org NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-27 BP S22 EP S22 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000052 ER PT J AU Reed, BM DeNoma, JS AF Reed, Barbara M. DeNoma, Jeanine S. TI Practical Aspects of Running DOE for Improving Growth Media for In Vitro Plants SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Reed, Barbara M.; DeNoma, Jeanine S.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-26 BP S21 EP S22 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000051 ER PT J AU Rupp, J Cruz, L Fellers, J Trick, H AF Rupp, Jessica Cruz, Luisa Fellers, John Trick, Harold TI RNAi Mediated Silencing of Endogenous Wheat Genes eIF4(iso)E2 and eIF4G Induces Resistance to Potyviruses Wheat Streak Mosaic Virus and Triticum Mosaic Virus. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica; Cruz, Luisa; Trick, Harold] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Fellers, John] USDA ARS, Washington, DC 20250 USA. EM jrupp@ksu.edu NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-1017 BP S37 EP S38 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000089 ER PT J AU Rupp, JL Cruz, L Fellers, JP Trick, HN AF Rupp, Jessica L. Cruz, Luisa Fellers, John P. Trick, Harold N. TI RNAi Mediated Silencing of Triticum Mosaic Virus Coat Protein Gene Induces Resistance to Virus in Transgenic Wheat. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica L.; Cruz, Luisa; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Fellers, John P.] USDA ARS, Hard Winter Wheat Genet Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66506 USA. EM jrupp@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2008 BP S51 EP S51 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000118 ER PT J AU Vanhove, A Niedz, R Pence, VC AF Vanhove, A. Niedz, R. Pence, V. C. TI Improving Growth of Quercus palustris Embryo Axes In Vitro: a DOE Approach. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Vanhove, A.; Pence, V. C.] Cincinnati Zoo & Bot Garden, Ctr Conservat & Res Endangered Wildlife, Cincinnati, OH 45220 USA. [Niedz, R.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM annecatherine.vanhove@cincinnatizoo.org NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2015 VL 51 SU 1 MA P-2001 BP S48 EP S48 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA CJ6HF UT WOS:000355594000111 ER PT J AU Yang, YL Nachman, RJ Pietrantonio, PV AF Yang, Yunlong Nachman, Ronald J. Pietrantonio, Patricia V. TI Molecular and pharmacological characterization of the Chelicerata pyrokinin receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Acari; Pyrokinin (PK)/pheromone biosynthesis activating neuropeptide (PBAN)/diapause hormone (DH); GPCR; Arthropod PRX-amide peptidomimetics; Tissue expression; Neuromedin U ID BIOSYNTHESIS-ACTIVATING NEUROPEPTIDE; PROTEIN-COUPLED RECEPTOR; DIAPAUSE HORMONE-RECEPTOR; EXPRESSED SEQUENCE TAGS; FUNCTIONAL-CHARACTERIZATION; HELICOVERPA-ZEA; BOMBYX-MORI; HELIOTHIS-VIRESCENS; PUPAL DIAPAUSE; PBAN RECEPTOR AB We identified the first pyrokinin receptor (Rhimi-PKR) in Chelicerata and analyzed structure-activity relationships of cognate ligand neuropeptides and their analogs. Based on comparative and phylogenetic analyses, this receptor, which we cloned from larvae of the cattle tick Rhipicephalus microplus (Acari: Ixodidae), is the ortholog of the insect pyrokinin (PK)/pheromone biosynthesis activating neuropeptide (PBAN)/diapause hormone (DH) neuropeptide family receptor. Rhimi-PKR functional analyses using calcium bioluminescence were performed with a developed stable recombinant CHO-RI cell line. Rhimi-PKR was activated by four endogenous PKs from the Lyme disease vector, the tick Ixodes scapularis (EC(50)s range: 85.4 nM-546 nM), and weakly by another tick PRX-amide peptide, periviscerokinin (PVK) (EC50 = 24.5 mu M). PR analogs with substitutions of leucine, isoleucine or valine at the C-terminus for three tick PR peptides, Ixosc-PK1, Ixosc-PK2, and Ixosc-PK3, retained their potency on Rhimi-PKR. Therefore, Rhimi-PKR is less selective and substantially more tolerant than insect PK receptors of C-terminal substitutions of leucine to isoleucine or valine, a key structural feature that serves to distinguish insect PR from PVK/CAP(2b) receptors. In females, ovary and synganglion had the highest Rhimi-PKR relative transcript abundance followed by the rectal sac, salivary glands, Malpighian tubules, and midgut. This is the first pharmacological analysis of a PK/PBAN/DH-like receptor from the Chelicerata, which will now permit the discovery of the endocrinological roles of this neuropeptide family in vectors of vertebrate pathogens. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Yang, Yunlong; Pietrantonio, Patricia V.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Nachman, Ronald J.] USDA, Southern Plains Agr Res Ctr, Insect Control & Cotton Dis Res Unit, College Stn, TX 77845 USA. RP Pietrantonio, PV (reprint author), Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. EM p-pietrantonio@tamu.edu FU NRI/CREES [2008-35302-18820]; Texas AgriLife Research Vector-Borne Disease Program seed grant; USDA/DOD DWFP [0500-32000-001-01R]; BARD [IS-4205-09C] FX The collaboration of Dr. Adalberto Perez de Leon, Director, Knipling-Bushland U.S. Livestock Insects Research Laboratory, USDA-ARS, Kerrville, Texas, USA, through the Materials Transfer Agreement between USDA ARS SPA and Texas AgriLife Research for obtaining tick tissues is appreciated. This research was partially supported by an award from the NRI/CREES (#2008-35302-18820) and a Texas AgriLife Research Vector-Borne Disease Program seed grant to P.V.P., and awards from USDA/DOD DWFP (#0500-32000-001-01R) [for the NOVOStar plate reader] and BARD (IS-4205-09C) [for the peptides] to R.J.N. NR 59 TC 3 Z9 3 U1 1 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAY PY 2015 VL 60 BP 13 EP 23 DI 10.1016/j.ibmb.2015.02.010 PG 11 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA CJ3BZ UT WOS:000355359700003 PM 25747529 ER PT J AU Burner, DM Legendre, BL Boykin, DL Duet, MJ AF Burner, David M. Legendre, Benjamin L. Boykin, Deborah L. Duet, Michael J., Jr. TI A retrospective analysis of genetic advance in natural ripening of sugarcane SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE Sugarcane; Fusarium sacchari; simulation techniques ID YIELD; REGISTRATION; LOUISIANA AB Natural ripening of sugarcane (Saccharum L. spp. hybrids) during late-summer and autumn is coincident with selection and harvesting, making ripening important from breeding and industrial perspectives. Our objective was to determine if natural ripening of sugarcane cultivars was affected by generation of recurrent selection. A retrospective analysis was conducted of field trials conducted annually from 1972 to 2012 near Houma, Louisiana. The significance and impact of the sampling date by generation interaction on stalk weight, Brix, sucrose and purity of normal juice (juice expressed by the mills or retained in the bagasse corrected for imbibition water) and sucrose yield (expressed as theoretical recoverable sucrose or simply, TRS) was examined in the first-ratoon crop sampled eight times yr(1) at 2-wk intervals from late-August to early-December. Stalk weight did not respond to selection. For any given sampling date, the most recent generation, Generation 7, had significantly greater normal juice Brix (NJB), normal juice sucrose (NJS) and normal juice purity (NJP) than Generation 2, but was not consistently greater than other generations of selection. There was no significant advance in mid- and late-season juice traits. Generation 7 was significantly greater than Generations 2 to 5 for TRS at each sampling date. Depending on the trait, comparable levels of NJB, NJS and NJP and TRS were expressed 2- to 8-wk earlier in Generation 7 than earlier generations, demonstrating a shift toward earlier natural ripening. Continued genetic advance seems likely for early-season NJB, NJS and NJP and early-, mid-, and late-season TRS. C1 [Burner, David M.; Duet, Michael J., Jr.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [Legendre, Benjamin L.] LSU AgCtr, Audubon Sugar Inst, St Gabriel, LA 70776 USA. [Boykin, Deborah L.] USDA ARS, Southeast Area, Stoneville, MS 38776 USA. RP Legendre, BL (reprint author), USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. EM BLegendre@agcenter.lsu.edu FU USDA-ARS [6052-21000-015-00D] FX USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. Technical expertise provided by field and juice laboratory personnel was greatly appreciated. Principle funding for the research was provided by USDA-ARS through Project 6052-21000-015-00D. NR 38 TC 0 Z9 0 U1 1 U2 1 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD MAY PY 2015 VL 117 IS 1397 BP 370 EP 377 PG 8 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA CJ3MM UT WOS:000355388100036 ER PT J AU Meneguzzo, DM Liknes, GC AF Meneguzzo, Dacia M. Liknes, Greg C. TI Status and Trends of Eastern Redcedar (Juniperus virginiana) in the Central United States: Analyses and Observations Based on Forest Inventory and Analysis Data SO JOURNAL OF FORESTRY LA English DT Article DE forest inventory; eastern redcedar; biological invasion; species diversity ID TALLGRASS PRAIRIE; RED-CEDAR; ENCROACHMENT; OKLAHOMA; USA; L. AB Eastern redcedar (Juniperus virginiana) (ERC) is a conifer native to North America that has been used for a variety of wood products, and its planting has been encouraged to help stabilize soil, reforest abandoned farmland, and provide cover for wildlife. However, ERC tends to expand rapidly and take over certain areas primarily because it can grow on a wide variety of soils and tolerates salt and harsh climatic conditions. As a result of this invasive behavior, the ERC composition of central United States forestlands has been on the rise over several decades. To evaluate the current status and recent trends of ERC on forestland in eight central states, we analyzed forest resource data collected annually from 2001 to 2012 by the Forest Inventory and Analysis (FIA) program of the USDA Forest Service at county, state, and regional levels. Our results indicate that ERC increased in terms of area, density, and volume across a range of diameter classes. In addition, ERC seedling abundance increased, and we found a statistically significant relationship between decreasing tree species diversity and ERC basal area proportion. We draw several conclusions from these analyses: (1) the geographic distribution of ERC in central US forestlands is widespread, but varies in density, (2) the area of ERC forestland increased most significantly in Nebraska and Missouri during the early 2000s, (3) the density and volume of ERC are on the rise in the region, and (4) the changes in seedling species abundance and the negative association between diversity and ERC presence suggest that the future composition of forests in the region could be altered if the current trends in ERC invasion continue. C1 [Meneguzzo, Dacia M.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. [Liknes, Greg C.] USDA, Forest Inventory & Anal, Forest Serv, St Paul, MN USA. RP Meneguzzo, DM (reprint author), US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. EM dmeneguzzo@fi.fed.us; gliknes@fs.fed.us NR 39 TC 0 Z9 0 U1 15 U2 40 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2015 VL 113 IS 3 BP 325 EP 334 DI 10.5849/jof.14-093 PG 10 WC Forestry SC Forestry GA CJ3IK UT WOS:000355376400007 ER PT J AU Craigg, TL Adams, PW Bennett, KA AF Craigg, Terry L. Adams, Paul W. Bennett, Karen A. TI Soil Matters: Improving Forest Landscape Planning and Management for Diverse Objectives with Soils Information and Expertise SO JOURNAL OF FORESTRY LA English DT Article DE soil quality; landscape planning; project planning; multiple uses and values; case study ID SPATIAL-PATTERNS; MULE DEER; RESTORATION; QUALITY; SCIENTISTS; RESILIENCE; CHALLENGES; VEGETATION; NORTHWEST; DENSITY AB Most forest managers would agree that soils are a fundamental resource of forestlands, yet many planning and management decisions continue to be made without a detailed and spatially explicit understanding of this unique and vital resource. We discuss the value of soil data and interpretations in forest planning. We emphasize that soil types differ widely in their inherent capacity to perform various ecological functions as well as in their dynamic response to and recovery from disturbances concepts that can greatly enhance the quality of forest management decisions. We make a case for applying these concepts by introducing an adaptive management model that targets the use of soil information during forest planning and management. Our goal is to help bridge the gap between soil science and decisionmaking by helping forest managers better understand the value of soil information in project planning. A case study highlights applications and potential benefits. C1 [Craigg, Terry L.] US Forest Serv, USDA, Deschutes Natl Forest, St Paul, MN 55108 USA. [Adams, Paul W.] Oregon State Univ, Corvallis, OR 97331 USA. [Bennett, Karen A.] US Forest Serv, USDA, St Paul, MN 55108 USA. RP Craigg, TL (reprint author), US Forest Serv, USDA, Deschutes Natl Forest, St Paul, MN 55108 USA. EM tcraigg@fs.fed.us; paul.adams@oregonstate.edu; kabennett@fs.fed.us FU USDA Forest Service; Oregon State University FX We gratefully acknowledge the support of the USDA Forest Service and Oregon State University in the development of this manuscript. NR 61 TC 1 Z9 1 U1 3 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2015 VL 113 IS 3 BP 343 EP 353 DI 10.5849/jof.14-083 PG 11 WC Forestry SC Forestry GA CJ3IK UT WOS:000355376400009 ER PT J AU Bragg, DC AF Bragg, Don C. TI Tree Improvement Program of the Crossett Experimental Forest SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 US Forest Serv, USDA, Southern Res Stn, Monticello, AR USA. RP Bragg, DC (reprint author), US Forest Serv, USDA, Southern Res Stn, Monticello, AR USA. EM dbragg@fs.fed.us NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2015 VL 113 IS 3 BP 354 EP 354 DI 10.5849/jof.15-016 PG 1 WC Forestry SC Forestry GA CJ3IK UT WOS:000355376400010 ER PT J AU Madsen-Bouterse, SA Schneider, DA Dassanayake, RP Truscott, TC Zhuang, DY Kumpula-McWhirter, N O'Rourke, KI AF Madsen-Bouterse, Sally A. Schneider, David A. Dassanayake, Rohana P. Truscott, Thomas C. Zhuang, Dongyue Kumpula-McWhirter, Nancy O'Rourke, Katherine I. TI PRNP variants in goats reduce sensitivity of detection of PrPSc by immunoassay SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Allelic variation; epitope; goats; immunoassay; immunohistochemistry; scrapie ID EXPERIMENTALLY CHALLENGED GOATS; MONOCLONAL-ANTIBODY F99/97.6.1; DEER ODOCOILEUS-HEMIONUS; CHRONIC WASTING DISEASE; PRION PROTEIN GENE; CLASSICAL SCRAPIE; LYMPHOID-TISSUE; NATURAL SCRAPIE; SHEEP; RESISTANCE AB Diagnostic analyses often employ single antibody systems but are potentially limited by epitope sequence variation. United States regulatory testing for scrapie primarily uses antibody F99/97.6.1 for immunohistochemistry (IHC) of the prion protein associated with scrapie (PrPSc). Whereas the epitope bound by F99/97.6.1 is highly conserved in sheep, a polymorphism in caprine PRNP results in a glutamine to lysine change at codon 222 and affects PrP detection. This study evaluated the performance of immunoassays (Western blot and IHC) in the presence of PRNP polymorphisms observed in U.S. goat populations. Effects of naturally occurring caprine prion protein alterations at codons 142, 143, 146, 154, or 222 were first evaluated using bacterially expressed recombinant normal cellular prion protein (rec-PrPC) and commercially available antibodies (F99/97.6.1, F89/160.1.5, L42, and SAF84). Detection of rec-PrPC using F89/160.1.5 was reduced by alterations at 142 and 143; this was also observed in brain PrPC from goats expressing these PRNP variants. Effect of allelic variation at 222 was confirmed by Western blot with F99/97.6.1. No differences were observed with L42 or SAF84. IHC of brain demonstrated reduced signal with F89/160.1.5 in animals heterozygous at 143. Decreasing F89/160.1.5 titers were used to demonstrate the impact of PrPSc immunolabeling in preclinical goats and as a surrogate for F99/97.6.1 detection in 222 variants. In the absence of epitope-relevant knowledge of individual goat PRNP, a multi-antibody approach or an antibody that binds an invariant site may provide a more robust immunoassay of PrPSc in classical scrapie, thus reducing the likelihood of false-negative results due to allelic variation. C1 [Madsen-Bouterse, Sally A.; Schneider, David A.; Dassanayake, Rohana P.; Kumpula-McWhirter, Nancy; O'Rourke, Katherine I.] Washington State Univ, Dept Vet Microbiol & Pathol, Coll Vet Med, Pullman, WA 99164 USA. [Schneider, David A.; Truscott, Thomas C.; Zhuang, Dongyue] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. RP Madsen-Bouterse, SA (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, 402 Bustad, Pullman, WA 99164 USA. EM smadsen@vetmed.wsu.edu FU USDA Agricultural Research Service [CRIS 2090-32000-030-00] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the USDA Agricultural Research Service under CRIS 2090-32000-030-00. NR 38 TC 1 Z9 1 U1 3 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD MAY PY 2015 VL 27 IS 3 BP 332 EP 343 DI 10.1177/1040638715585865 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA CJ7RH UT WOS:000355695500009 PM 26038481 ER PT J AU Parsons, J Matthews, W Iorizzo, M Roberts, P Simon, P AF Parsons, Joshua Matthews, William Iorizzo, Massimo Roberts, Philip Simon, Philipp TI Meloidogyne incognita nematode resistance QTL in carrot SO MOLECULAR BREEDING LA English DT Article DE Carrot; Root-knot nematodes; QTL; Disease resistance; Daucus carota L. ID ROOT-KNOT NEMATODE; JAVANICA RESISTANCE; CONFERS RESISTANCE; GENETIC DIVERSITY; HIGH-DENSITY; DNA MARKERS; LINKAGE; TOMATO; INHERITANCE; REPRODUCTION AB Root-knot nematodes (Meloidogyne spp.) are major pests attacking carrots (Daucus carota) worldwide, causing galling and forking of the storage roots, rendering them unacceptable for market. Genetic resistance could significantly reduce the need for broad-spectrum soil fumigants in carrot production. In this study, genetic resistance to Meloidogyne incognita was mapped. Three diverse sources of resistance, from Syria (HM), Europe (SFF) and South America (Br1091), were identified. Two F-2 mapping populations were developed using these parents, (Br1091 x HM1) and (SFF x HM2), as well as a segregating population derived from the self-pollination of a HM plant (HM3). Analysis revealed four QTLs conditioning resistance in Br1091 x HM1, three in SFF x HM2, and three in HM3. A consensus genetic map of the three populations revealed five non-overlapping QTLs for M. incognita resistance, one each on carrot chromosomes 1, 2, 4, 8, and 9. One QTL was present in all three populations, in the same region of chromosome 8 as Mj-1 which imparts resistance to M. javanica. C1 [Parsons, Joshua; Iorizzo, Massimo; Simon, Philipp] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Matthews, William; Roberts, Philip] Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA. [Simon, Philipp] ARS, Vegetable Crops Res Unit, USDA, Madison, WI 53706 USA. RP Simon, P (reprint author), ARS, Vegetable Crops Res Unit, USDA, 1575 Linden Dr, Madison, WI 53706 USA. EM philipp.simon@ars.usda.gov FU California Fresh Carrot Advisory Board; Specialty Crop Research Initiative award [2008-51180-04896]; Organic Agriculture Research and Extension Initiative award [2011-51300-30903]; Monsanto Graduate Research Fellowship through the University of Wisconsin-Madison Plant Breeding and Plant Genetics Program FX This work was funded by the California Fresh Carrot Advisory Board grants (to PWS and PAR); Specialty Crop Research Initiative award 2008-51180-04896 (to PWS and PAR); Organic Agriculture Research and Extension Initiative award 2011-51300-30903 (to PWS and PAR); and a Monsanto Graduate Research Fellowship to J.P. through the University of Wisconsin-Madison Plant Breeding and Plant Genetics Program. The authors appreciate the capable assistance provided by Dr. Doug Senalik. NR 52 TC 0 Z9 0 U1 1 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD MAY PY 2015 VL 35 IS 5 AR 114 DI 10.1007/s11032-015-0309-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CJ4GG UT WOS:000355442200003 ER PT J AU Schneider, AD Nava, IC Herve, CB Islamovic, E Limberger, E Jackson, EW Delatorre, CA AF Schneider, Adriano de Bernardi Nava, Itamar Cristiano Herve, Cassio Barcellos Islamovic, Emir Limberger, Emerson Jackson, Eric W. Delatorre, Carla Andrea TI Chromosome-anchored QTL conferring aluminum tolerance in hexaploid oat SO MOLECULAR BREEDING LA English DT Article DE Avena sativa; Genetic map; Quantitative trait loci; Aluminum tolerance; Root regrowth ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; HORDEUM-VULGARE L.; CULTIVATED OAT; AVENA-SATIVA; LINKAGE MAP; ACID SOILS; ALMT1 GENE; MATE GENE; RESISTANCE AB Aluminum (Al) toxicity is a major constraint on crop production in acid soils around the world. Hexaploid oat (Avena sativa L.) possesses significant Al tolerance making it a good candidate for production in these environments. Genetic improvement for Al tolerance in oat has traditionally been achieved through conventional plant breeding and could be enhanced by marker-assisted selection. The objectives of this study were to develop a chromosome-anchored genetic map for an oat recombinant inbred population and to identify SNP markers linked to quantitative trait loci (QTL) affecting root growth response to Al. Three QTL on chromosomes 7C-17A, 13A, and 19A conferring Al tolerance were identified using primary root regrowth of recombinant inbred lines derived from the cross between UFRGS 17 (Al tolerant) and UFRGS 930598-6 (Al sensitive). Localization of each QTL onto the sequenced rice genome revealed the genetic region on chromosome 13A might be associated with a putative malate transporter locus (LOC_Os06g15779). Studies of root apex tissue indicated that exudation of malate was increased in the Al-tolerant parent UFRGS17 and not in the sensitive parent. Based on these data, the malate transporter might be a candidate gene responsible for one of the Al tolerance QTL identified in this study. C1 [Schneider, Adriano de Bernardi; Nava, Itamar Cristiano; Herve, Cassio Barcellos; Islamovic, Emir; Delatorre, Carla Andrea] Fed Univ Rio Grande Sul UFRGS, Dept Crop Sci, BR-91501970 Porto Alegre, RS, Brazil. [Islamovic, Emir] ARS, Small Grains & Potato Germplasm Res Unit, USDA, Aberdeen, ID 83210 USA. [Jackson, Eric W.] Gen Mills Crop Biosci, Manhattan, KS 66502 USA. RP Delatorre, CA (reprint author), Fed Univ Rio Grande Sul UFRGS, Dept Crop Sci, Av Bento Goncalves 7712,POB 15100, BR-91501970 Porto Alegre, RS, Brazil. EM cadtorre@ufrgs.br RI de Bernardi Schneider, Adriano/E-6252-2017 OI de Bernardi Schneider, Adriano/0000-0001-7487-266X FU Brazilian National Council for Scientific and Technological Development (CNPq); Coordination for the Improvement of Higher Education Personnel (CAPES) from Brazil; Prairie Oat Growers Association; US Department of Agriculture FX The authors thank Dr. Eric Jellen and Dr. Jeff Maughan from Brigham Young University for the support during the SNP analysis; Dr. Marcelo T. Pacheco for the heritability analysis, and Mauricio D. Salomon for figure preparation. This study was supported by grants, scholarships, and fellowships from the Brazilian National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES) from Brazil, The Prairie Oat Growers Association, and the US Department of Agriculture. NR 70 TC 0 Z9 0 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD MAY PY 2015 VL 35 IS 5 AR UNSP 121 DI 10.1007/s11032-015-0315-4 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CJ4GG UT WOS:000355442200010 ER PT J AU Yauk, CL Aardema, MJ van Benthem, J Bishop, JB Dearfield, KL DeMarini, DM Dubrova, YE Honma, M Lupski, JR Marchetti, F Meistrich, ML Pacchierotti, F Stewart, J Waters, MD Douglas, GR AF Yauk, Carole L. Aardema, Marilyn J. van Benthem, Jan Bishop, Jack B. Dearfield, Kerry L. DeMarini, David M. Dubrova, Yuri E. Honma, Masamitsu Lupski, James R. Marchetti, Francesco Meistrich, Marvin L. Pacchierotti, Francesca Stewart, Jane Waters, Michael D. Douglas, George R. TI Approaches for identifying germ cell mutagens: Report of the 2013 IWGT workshop on germ cell assays SO MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS LA English DT Article; Proceedings Paper CT 6th International Workshop on Genotoxicity Testing (IWGT) CY OCT 31-NOV 01, 2013 CL Foz do Iguacu, BRAZIL DE Germ cell mutation; Genetic disease; Reproductive health; Copy number variants; Gene; mutation; Chromosome aberrations ID COPY-NUMBER VARIANTS; BLOOD-TESTIS BARRIER; MARROW MICRONUCLEUS TEST; REPRODUCTIVE END-POINTS; DOMINANT LETHAL ASSAY; SPERM DNA-DAMAGE; RISK-ASSESSMENT; BONE-MARROW; HUMAN GENOME; IN-VIVO AB This workshop reviewed the current science to inform and recommend the best evidence-based approaches on the use of germ cell genotoxicity tests. The workshop questions and key outcomes were as follows. (1) Do genotoxicity and mutagenicity assays in somatic cells predict germ cell effects? Limited data suggest that somatic cell tests detect most germ cell mutagens, but there are strong concerns that dictate caution in drawing conclusions. (2) Should germ cell tests be done, and when? If there is evidence that a chemical or its metabolite(s) will not reach target germ cells or gonadal tissue, it is not necessary to conduct germ cell tests, notwithstanding somatic outcomes. However,,it was recommended that negative somatic cell mutagens with clear evidence for gonadal exposure and evidence of toxicity in germ cells could be considered for germ cell mutagenicity testing. For somatic mutagens that are known to reach the gonadal compartments and expose germ cells, the chemical could be assumed to be a germ cell mutagen without further testing. Nevertheless, germ cell mutagenicity testing would be needed for quantitative risk assessment. (3) What new assays should be implemented and how? There is an immediate need for research on the application of whole genome sequencing in heritable mutation analysis in humans and animals, and integration of germ cell assays with somatic cell genotoxicity tests. Focus should be on environmental exposures that can cause de novo mutations, particularly newly recognized types of genomic changes. Mutational events, which may occur by exposure of germ cells during embryonic development, should also be investigated. Finally, where there are indications of germ cell toxicity in repeat dose or reproductive toxicology tests, consideration should be given to leveraging those studies to inform of possible germ cell genotoxicity. Crown Copyright (C) 2015 Published by Elsevier B.V. C1 [Yauk, Carole L.; Marchetti, Francesco; Douglas, George R.] Hlth Canada, Environm Hlth Sci & Res Bur, Ottawa, ON K1A 0L2, Canada. [Aardema, Marilyn J.] Marilyn Aardema Consulting, Fairfield, OH USA. [van Benthem, Jan] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands. [Bishop, Jack B.] Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA. [Dearfield, Kerry L.] USDA, Food Safety & Inspect Serv, Washington, DC 20250 USA. [DeMarini, David M.] Univ N Carolina, Chapel Hill, NC 27515 USA. [Dubrova, Yuri E.] Univ Leicester, Leicester LE1 7RH, Leics, England. [Honma, Masamitsu] Natl Inst Hlth Sci, Tokyo, Japan. [Lupski, James R.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Lupski, James R.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Meistrich, Marvin L.] Univ Texas MD Anderson Canc Ctr, Houston, TX USA. [Marchetti, Francesco] Italian Natl Agcy New Technol, ENEA, Energy & Sustainable Econ Dev, Milan, Italy. [Stewart, Jane] AstraZeneca, Bangalore, Karnataka, India. [Waters, Michael D.] Integrated Lab Syst Inc, Res Triangle Pk, NC USA. RP Yauk, CL (reprint author), Hlth Canada, Environm Hlth Sci & Res Bur, Ottawa, ON K1A 0L2, Canada. EM Carole.Yauk@hc-sc.gc.ca; George.Douglas@hc-sc.gc.ca OI Dubrova, Yuri/0000-0001-5281-7539; Marchetti, Francesco/0000-0002-9435-4867; Yauk, Carole/0000-0003-4919-876X FU IWGT FX Funding for this workshop was provided by the IWGT. NR 165 TC 13 Z9 13 U1 3 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1383-5718 EI 1879-3592 J9 MUTAT RES-GEN TOX EN JI Mutat. Res. Genet. Toxicol. Environ. Mutagen. PD MAY 1 PY 2015 VL 783 SI SI BP 36 EP 54 DI 10.1016/j.mrgentox.2015.01.008 PG 19 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology SC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology GA CJ2YX UT WOS:000355351700006 PM 25953399 ER PT J AU Desjardins, CA Sanscrainte, ND Goldberg, JM Heiman, D Young, S Zeng, QD Madhani, HD Becnel, JJ Cuomo, CA AF Desjardins, Christopher A. Sanscrainte, Neil D. Goldberg, Jonathan M. Heiman, David Young, Sarah Zeng, Qiandong Madhani, Hiten D. Becnel, James J. Cuomo, Christina A. TI Contrasting host-pathogen interactions and genome evolution in two generalist and specialist microsporidian pathogens of mosquitoes SO NATURE COMMUNICATIONS LA English DT Article ID PARASITE ENCEPHALITOZOON-CUNICULI; EDHAZARDIA-AEDIS; AEDES-AEGYPTI; SEQUENCE; ANNOTATION; ALIGNMENT; CULICIDAE; PROTEINS; INSIGHTS; DIPTERA AB Obligate intracellular pathogens depend on their host for growth yet must also evade detection by host defenses. Here we investigate host adaptation in two Microsporidia, the specialist Edhazardia aedis and the generalist Vavraia culicis, pathogens of disease vector mosquitoes. Genomic analysis and deep RNA-Seq across infection time courses reveal fundamental differences between these pathogens. E. aedis retains enhanced cell surface modification and signalling capacity, upregulating protein trafficking and secretion dynamically during infection. V. culicis is less dependent on its host for basic metabolites and retains a subset of spliceosomal components, with a transcriptome broadly focused on growth and replication. Transcriptional profiling of mosquito immune responses reveals that response to infection by E. aedis differs dramatically depending on the mode of infection, and that antimicrobial defensins may play a general role in mosquito defense against Microsporidia. This analysis illuminates fundamentally different evolutionary paths and host interplay of specialist and generalist pathogens. C1 [Desjardins, Christopher A.; Goldberg, Jonathan M.; Heiman, David; Young, Sarah; Zeng, Qiandong; Cuomo, Christina A.] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA. [Sanscrainte, Neil D.; Becnel, James J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Madhani, Hiten D.] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA. RP Becnel, JJ (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM James.Becnel@ars.usda.gov; cuomo@broadinstitute.org OI Cuomo, Christina/0000-0002-5778-960X FU National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN272200900018C]; USDA-Agricultural Research Service FX We acknowledge the Broad Institute Sequencing Platform for generating all DNA and RNA sequences described here. We thank Narmada Shemoy for assistance with gene prediction, Michelle Busby for assistance with RNA-Seq quality metrics and Rhys Farrer and Abigail McGuire for helpful comments on the manuscript. This project was funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900018C. This research was also supported by the USDA-Agricultural Research Service. NR 80 TC 13 Z9 15 U1 7 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2015 VL 6 AR 7121 DI 10.1038/ncomms8121 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ5MW UT WOS:000355533000009 PM 25968466 ER PT J AU Jung, YH Chang, TH Zhang, HL Yao, CH Zheng, QF Yang, VW Mi, HY Kim, M Cho, SJ Park, DW Jiang, H Lee, J Qiu, YJ Zhou, WD Cai, ZY Gong, SQ Ma, ZQ AF Jung, Yei Hwan Chang, Tzu-Hsuan Zhang, Huilong Yao, Chunhua Zheng, Qifeng Yang, Vina W. Mi, Hongyi Kim, Munho Cho, Sang June Park, Dong-Wook Jiang, Hao Lee, Juhwan Qiu, Yijie Zhou, Weidong Cai, Zhiyong Gong, Shaoqin Ma, Zhenqiang TI High-performance green flexible electronics based on biodegradable cellulose nanofibril paper SO NATURE COMMUNICATIONS LA English DT Article ID LIGHT-EMITTING-DIODES; ORGANIC SOLAR-CELLS; THERMAL-CONDUCTIVITY; GALLIUM-ARSENIDE; TRANSPARENT; TOXICITY; FIBER; NANOCOMPOSITES; TRANSISTORS; COMPOSITES AB Today's consumer electronics, such as cell phones, tablets and other portable electronic devices, are typically made of non-renewable, non-biodegradable, and sometimes potentially toxic (for example, gallium arsenide) materials. These consumer electronics are frequently upgraded or discarded, leading to serious environmental contamination. Thus, electronic systems consisting of renewable and biodegradable materials and minimal amount of potentially toxic materials are desirable. Here we report high-performance flexible microwave and digital electronics that consume the smallest amount of potentially toxic materials on biobased, biodegradable and flexible cellulose nanofibril papers. Furthermore, we demonstrate gallium arsenide microwave devices, the consumer wireless workhorse, in a transferrable thin-film form. Successful fabrication of key electrical components on the flexible cellulose nanofibril paper with comparable performance to their rigid counterparts and clear demonstration of fungal biodegradation of the cellulose-nanofibril-based electronics suggest that it is feasible to fabricate high-performance flexible electronics using ecofriendly materials. C1 [Jung, Yei Hwan; Chang, Tzu-Hsuan; Zhang, Huilong; Mi, Hongyi; Kim, Munho; Cho, Sang June; Park, Dong-Wook; Jiang, Hao; Lee, Juhwan; Qiu, Yijie; Ma, Zhenqiang] Univ Wisconsin, Dept Elect & Comp Engn, Madison, WI 53706 USA. [Yao, Chunhua; Zheng, Qifeng; Gong, Shaoqin] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53706 USA. [Yang, Vina W.; Cai, Zhiyong] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Qiu, Yijie] Univ Elect Sci & Technol China, Sch Elect Engn, Chengdu 611731, Peoples R China. [Zhou, Weidong] Univ Texas Arlington, Dept Elect Engn, Arlington, TX 76019 USA. [Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA. [Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53706 USA. RP Ma, ZQ (reprint author), Univ Wisconsin, Dept Elect & Comp Engn, 1415 Engn Dr,3445 Engn Hall, Madison, WI 53706 USA. EM sgong@engr.wisc.edu; mazq@engr.wisc.edu FU Air Force Office of Scientific Research (AFOSR): Presidential Early Career Award in Science & Engineering (PECASE) grant [FA9550-09-1-0482] FX This work was supported by the Air Force Office of Scientific Research (AFOSR): Presidential Early Career Award in Science & Engineering (PECASE) grant # FA9550-09-1-0482. The program manager is Dr Gernot Pomrenke. NR 45 TC 72 Z9 74 U1 51 U2 188 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2015 VL 6 AR 7170 DI 10.1038/ncomms8170 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CJ5NK UT WOS:000355534400007 PM 26006731 ER PT J AU Davies, KW Boyd, CS Johnson, DD Nafus, AM Madsen, MD AF Davies, K. W. Boyd, C. S. Johnson, D. D. Nafus, A. M. Madsen, M. D. TI Success of Seeding Native Compared with Introduced Perennial Vegetation for Revegetating Medusahead-Invaded Sagebrush Rangeland SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Bunchgrasses; Crested wheatgrass; Exotic annual grasses; Forage kochia; Imazapic; Restoration; Wyoming big sagebrush ID EXOTIC ANNUAL GRASS; GREAT-BASIN; CRESTED WHEATGRASS; PLANT-COMMUNITIES; BROMUS-TECTORUM; DOWNY BROME; STEPPE; ESTABLISHMENT; RESTORATION; ECOSYSTEMS AB Millions of hectares of Wyoming big sagebrush (Artemisia tridentata Nutt. subsp. wyomingensis Beetle&Young) rangeland have been invaded by medusahead (Taeniatherum caput-medusae [L] Nevski), an exotic annual grass that degrades wildlife habitat, reduces forage production, and decreases biodiversity. Revegetation of medusahead-invaded sagebrush plant communities is necessary to restore ecosystem services. Disagreement, however, exists over whether to seed native or introduced perennial species to revegetate communities after controlling medusahead. Though native species generally do not establish as well as introduced species, interference from co-seeded introduced species has often been attributed to the limited success of natives. The potential for seeding natives to revegetate communities after medusahead control is relatively unknown because they have been largely co-seeded with introduced species. We compared the results of seeding native and introduced perennial species after controlling medusahead with prescribed burning followed with an imazapic herbicide application at five sites. Perennial bunchgrass cover and density were 5- and 10-fold greater in areas seeded with introduced compared with native species 3 years post seeding. Furthermore, exotic annual grass cover and density were less in areas seeded with introduced compared with native species. Seeded introduced and native shrubs largely failed to establish. High perennial bunchgrass density (15 individuals . m(-2)) in areas seeded with introduced species in the third year post seeding suggests that the succession trajectory of these communities has shifted to becoming perennial dominated. Average perennial bunchgrass density of 1.5 individuals . m(-2) with seeding native species will likely not limit medusahead and appears to already be converting back to exotic annual grass-dominated communities. These results suggest that seeding introduced compared with native species after medusahead control will likely be more successful. Our results also imply that if natives are selected to seed after medusahead control, additional resources may be necessary to recontrol medusahead and repeatedly sow native species. Published by Elsevier Inc. On behalf of Society for Range Management. C1 [Davies, K. W.; Boyd, C. S.; Madsen, M. D.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Johnson, D. D.; Nafus, A. M.] Oregon State Univ, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Davies, KW (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu FU Oregon Department of Fish and Wildlife - USFWS - Wildlife and Sportfish Restoration program; USDA-Agricultural Research Service FX Research was funded by Oregon Department of Fish and Wildlife funded in part by the USFWS - Wildlife and Sportfish Restoration program and USDA-Agricultural Research Service. NR 49 TC 5 Z9 7 U1 6 U2 26 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2015 VL 68 IS 3 BP 224 EP 230 DI 10.1016/j.rama.2015.03.004 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ8XR UT WOS:000355787300002 ER PT J AU Madsen, MD Zvirzdin, DL Petersen, SL Hopkins, BG Roundy, BA AF Madsen, M. D. Zvirzdin, D. L. Petersen, S. L. Hopkins, B. G. Roundy, B. A. TI Anchor Chaining's Influence on Soil Hydrology and Seeding Success in Burned Pinon-Juniper Woodlands SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE anchor chaining; seeding; soil water repellency; restoration; wildfire ID WATER REPELLENCY; HYDROPHOBIC SUBSTANCES; SAGEBRUSH ECOSYSTEMS; PLANT-COMMUNITIES; WETTING AGENT; GREAT-BASIN; VEGETATION; ESTABLISHMENT; INFILTRATION; EROSION AB Broadcast seeding is one of the most commonly applied rehabilitation treatments for the restoration of burned pinon and juniper woodlands, but the success rate of this treatment is notoriously low. In pinon-juniper woodlands, postfire soil water repellency can impair seeding success by reducing soil water content and increasing soil erosion. Implementing anchor chaining immediately after seeding can improve establishment of seeded species by enhancing seed-to-soil contact and may improve restoration success by decreasing soil water repellency through soil tillage. The objectives of this research were to 1) determine if anchor chaining in postfire pinyon-juniper woodlands diminishes soil water repellency, and 2) determine meaningful relationships between soil water repellency, unsaturated hydraulic conductivity [K(h)], and the establishment of seeded and invasive species. Research was conducted on two study sites, each located on a burned pinon-juniper woodland that had severe water repellency and that was aerially seeded. At each location, plots were randomly located in similar ecological sites of chained and unchained areas. At one location, anchor chaining considerably improved soil hydrologic properties, reducing the severity and thickness of the water-repellent layer, and increasing soil K(h) 2- to 4-fold in the first 2 yr following treatment At this same location, anchor chaining increased perennial grass cover 16-fold and inhibited annual grass and annual forb cover by 5- and 7-fold, respectively. Results from the second site only showed improvements in soil K(h); other hydrologic and vegetative treatment responses were not significantly improved. Overall, this research suggests that anchor chaining has the potential to improve restoration outcomes, though additional research is warranted for understanding the direct impact of anchor chaining on soil water repellency without the interaction of a seeding treatment. Published by Elsevier Inc. On behalf of Society for Range Management C1 [Madsen, M. D.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Zvirzdin, D. L.; Petersen, S. L.; Hopkins, B. G.; Roundy, B. A.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. RP Madsen, MD (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. EM matthew.madsen@oregonstate.edu FU USDA-National Institute of Food and Agriculture's Rangeland Research Program; USDA-NRCS Conservation Initiative Gant; USDA-ARS FX Funding for this research was provided by USDA-National Institute of Food and Agriculture's Rangeland Research Program, the USDA-NRCS Conservation Initiative Gant and the USDA-ARS. We would like to thank Scott Chamberlain for providing logistical support to conduct research on Utah School and Institutional Trust Lands and for various personnel with the Bureau of Land Management for providing support on federal land. We are grateful to Aaron Inouye, Kaitlynn Fernelius, Ben Stearns, Alexander Zvirzdin, and Eric Gardner who aided in collecting and processing of field data. We are also grateful to Chad Boyd and Kirk Davies for reviewing earlier versions of this manuscript. USDA is an equal opportunity provider and employer. NR 72 TC 2 Z9 2 U1 1 U2 13 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2015 VL 68 IS 3 BP 231 EP 240 DI 10.1016/j.rama.2015.03.010 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ8XR UT WOS:000355787300003 ER PT J AU Warren, SD St Clair, LL Johansen, JR Kugrens, P Baggett, LS Bird, BJ AF Warren, Steven D. St Clair, Larry L. Johansen, Jeffrey R. Kugrens, Paul Baggett, L. Scott Bird, Benjamin J. TI Biological Soil Crust Response to Late Season Prescribed Fire in a Great Basin Juniper Woodland SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE cyanobacteria; green algae; lichens; mosses; nitrogen fixation; prescribed burn ID COLUMBIA BASIN; RECOVERY; UTAH; COMMUNITY; WILDFIRE; MONTANA; STEPPE; USA AB Expansion of juniper on U.S. rangelands is a significant environmental concern. Prescribed fire is often recommended to control juniper. To that end, a prescribed burn was conducted in a Great Basin juniper woodland. Conditions were suboptimal; fire did not encroach into mid- or late-seral stages and was patchy in the early-seral stage. This study evaluated the effects of the burn on biological soil crusts of early-seral juniper. Fire reduced moss cover under sagebrush and in shrub interspaces. Mosses were rare under juniper; their cover was unaffected there. Lichens were uncommon under juniper and sagebrush and therefore not significantly impacted there. Their cover was greater in shrub interspaces, but because the fire was spotty and of low intensity, the effects of burning were minimal. Compared with unburned plots, the biomass of cyanobacteria was diminished under juniper and sagebrush; it was reduced in the interspaces in both burned and unburned plots, presumably in response to generally harsher conditions in the postburn environment. Nitrogen fixation rates declined over time in juniper plots and interspaces but not in sagebrush plots. Although fire negatively affected some biological soil crust organisms in some parts of the early-seral juniper woodland, the overall impact on the crusts was minimal. If the intent of burning is to reduce juniper, burning of early-seral juniper woodland is appropriate, as most affected trees were killed. Control of sagebrush can likewise be accomplished by low-intensity, cool season fires without eliminating the crust component. Intense fire should be avoided due to the potential for greater encroachment into the shrub interspaces, which contain the majority of biological soil crust organisms. Burning early-seral juniper may be preferred for controlling juniper encroachment on rangeland. Published by Elsevier Inc. On behalf of Society for Range Management. C1 [Warren, Steven D.] US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. [St Clair, Larry L.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Johansen, Jeffrey R.] John Carroll Univ, Dept Biol, University Hts, OH 44118 USA. [Kugrens, Paul] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Baggett, L. Scott; Bird, Benjamin J.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Warren, SD (reprint author), US Forest Serv, Shrub Sci Lab, 735 N 500 E, Provo, UT 84606 USA. EM swarren02@fs.fed.us FU multiagency Joint Fire Science Program FX Research was funded by the multiagency Joint Fire Science Program. NR 44 TC 0 Z9 1 U1 2 U2 25 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2015 VL 68 IS 3 BP 241 EP 247 DI 10.1016/j.rama.2015.03.007 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ8XR UT WOS:000355787300004 ER PT J AU Roche, LM Cutts, BB Derner, JD Lubell, MN Tate, KW AF Roche, L. M. Cutts, B. B. Derner, J. D. Lubell, M. N. Tate, K. W. TI On-Ranch Grazing Strategies: Context for the Rotational Grazing Dilemma SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE attitudes; conditional inference regression tree analysis; decision-making; grazing management practices; grazing system; latent class analysis; prescribed grazing ID MANAGEMENT-PRACTICES; REGRESSION TREES; DECISION-MAKING; LOCAL KNOWLEDGE; RANDOM FORESTS; CLASSIFICATION; PROGRAM; POLICY; TEXAS; LAND AB Considerable debate remains over the efficacy of rotational grazing systems to enhance conservation and agricultural production goals on rangelands. We analyzed responses to grazing management questions in the Rangeland Decision Making Surveys of 765 California and Wyoming ranchers in order to characterize on-ranch grazing strategies and identify variables influencing strategy adoption. Two-thirds of respondents practice on-ranch rotational grazing strategies, indicating ranchers do experience benefits from rotation which have not been documented in experimental comparisons of rotational and continuous grazing systems. Limited on-ranch adoption of intensive rotational strategies (5% of respondents) indicates potential agreement between research and management perceptions about the success of this particular strategy for achieving primary livestock production goals. Over 93% of all rotational grazer respondents were characterized as using extensive intragrowing season rotation with moderate (few wk to mo) grazing period durations, moderate (2.4-8 ha.animal unit) livestock densities, and growing season rest periods. Variables associated with ranchers' grazing preferences included a mixture of human dimensions (goal setting, views on experiment and risk tolerance, information networks), ranch characteristics (total number of livestock, land types comprising ranch), and ecoregions. We also found that the majority of grazing systems research has largely been conducted at spatial and temporal scales that are orders of magnitude finer than conditions under which on-ranch adaptive grazing management strategies have been developed. Resolving the discrepancies between the grazing systems research and management knowledge base will require substantive communication and novel approaches to participatory research between scientists and managers. (C) 2015 Society for Range Management. Published by Elsevier Inc. All rights reserved. C1 [Roche, L. M.; Tate, K. W.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Cutts, B. B.] Univ Illinois, Dept Nat Resources & Environm Sci, Champaign, IL 61820 USA. [Derner, J. D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. [Lubell, M. N.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. RP Roche, LM (reprint author), Univ Calif Davis, Dept Plant Sci, Mail Stop 1, Davis, CA 95616 USA. EM lmroche@ucdavis.edu FU USDA-NIFA, Rangeland Research Program [2009-38415-20265]; Western Sustainable Agriculture, Research, and Education program [SW10-073] FX Research was funded by USDA-NIFA, Rangeland Research Program (Grant No. 2009-38415-20265); and Western Sustainable Agriculture, Research, and Education program (Project Number SW10-073). NR 47 TC 4 Z9 4 U1 5 U2 50 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2015 VL 68 IS 3 BP 248 EP 256 DI 10.1016/j.rama.2015.03.011 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ8XR UT WOS:000355787300005 ER PT J AU Kronberg, SL AF Kronberg, S. L. TI Improving Cattle Nutrition on the Great Plains with Shrubs and Fecal Seeding of Fourwing Saltbush SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE dormant-season; grazing; protein intake; winter; winterfat ID CRESTED WHEATGRASS; RANGE FORAGE; GRASS; DIGESTIBILITY; SASKATCHEWAN; RECRUITMENT; GERMINATION; WINTERFAT; LIVESTOCK; RECOVERY AB Two in vitro trials were conducted for estimates of dietary percentage of fourwing saltbush (Atriplex canescens; FS) or winterfat (Krascheninnikovia lanata; WF) for improved dietary digestibility when cattle graze mature cool-season grass. Three in vitro trials were conducted to estimate percentage of FS and WF seeds that could survive passage through the bovine gastro-intestinal tract (GIT) with potential for fecal seeding. Mixtures of FS and mature smooth bromegrass (Bromus inermis; SB) or WF and SB had greater apparent digestibility than SB alone (P < 0.0001). There were positive linear relationships (r(2) >= 0.93) between the amount of each shrub in digested mixtures and digestibility. Similar relationships were observed with mixtures of FS and mature Altai wildrye (Leymus angustus). Germination of Dakota FS seeds in the first trial, incubated for 24 or 48 h, was 55% and 47%, respectively, with no difference in germination of seeds for the 24- and 48-h incubations (P = 0.26), but more seeds germinated if incubated versus not (P <= 0.002). Germination of Utah FS seeds, which were incubated for 24 or 48 h with high-, medium-, or low-quality forage, averaged 9% and 8%, respectively. Length of incubation, forage quality, and their interaction did not influence germination (P >= 0.45). Germination of nonincubated Utah FS seeds was 21% and greater than for incubated seeds (P = 0.004). Average germination of WF seeds was 0.6% and 0.1% for 24- and 48-h incubations, respectively, with incubation length, forage quality, and their interaction not significant (P >= 0.31). Nonincubated WF seeds had greater germination (42%) than incubated seeds (P <0.0001). Results from the third trial were confirmatory for Dakota FS seed. FS and WF can improve diet quality of grazing cattle in late summer through winter, and some FS seeds have potential for fecal seeding. Published by Elsevier Inc. On behalf of Society for Range Management. C1 USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Kronberg, SL (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM scott.kronberg@ars.usda.gov NR 33 TC 2 Z9 2 U1 1 U2 5 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2015 VL 68 IS 3 BP 285 EP 289 DI 10.1016/j.rama.2015.03.003 PG 5 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CJ8XR UT WOS:000355787300009 ER PT J AU Logsdon, SD AF Logsdon, Sally D. TI Event- and Site-Specific Soil Wetting and Seasonal Change in Amount of Soil Water SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID COLOR PATTERNS; CENTRAL IOWA; HYDRAULIC CONDUCTIVITY; MOISTURE REGIMES; TABLE RESPONSE; STABILITY; VARIABILITY; YIELD; TIME; AIR AB Changes in the amount of soil water might not be uniform due to spatially variable soil and landscape factors. The objective of this study was to determine if the changes in the amount of soil water due to rain or during sub-seasonal times are related to soil or landscape properties within a field. Sub-seasonal changes in soil water were indicated by neutron probe data and surface soil sampling, and rain-event-driven soil water increases were indicated by water content reflectometers (WCRs). Excess rainfall in 2010 resulted in wet soil and surface ponding at some sites. Soil zones satiated with water were apparent from WCR data, especially for concave and depressional sites. The length of time soil remained satiated at the 0.3- and 0.6-m depths was negatively correlated with relative elevation (in relation to the nearest depression edge). Soil water increases for the 0.015- to 0.15-m depth were most often correlated with pre-rain soil water deficit, especially in the wet year of 2010 (13 of 14 events). Change in the amount of soil water at the deepest depth for the later part of the 2012 season (a drought year) was negatively correlated with profile and plan curvatures and mollic depth and positively correlated with relative elevation and sand fraction, which was the opposite of the expected trend. Two sites with buried sand lenses took the longest time to reach maximum soil water increase, suggesting that further study is needed to describe how buried sand lenses affect wetting in the soil above the sand lens. C1 USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Logsdon, SD (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM sally.logsdon@ars.usda.gov FU NASA Terrestrial Hydrology Program [NNG06GC63G] FX I acknowledge helpful internal review comments from Amy Kaleita and Mahmood Nachabe. Thanks to Brian Hornbuckle and the project (NASA Terrestrial Hydrology Program, NNG06GC63G) that funded equipment for this study. Thanks to Gavin Simmons for collection of field data. NR 66 TC 1 Z9 1 U1 2 U2 9 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 730 EP 741 DI 10.2136/sssaj2014.08.0327 PG 12 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000003 ER PT J AU Allred, BJ Brown, GO Martinez, LR AF Allred, Barry J. Brown, Glenn O. Martinez, Luis R. TI Laboratory Investigation of Boundary Condition Impacts on Nitrate Anion Exclusion in an Unsaturated Soil SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID CLAY SOIL; FLOW CONDITIONS; POROUS-MEDIA; WATER-FLOW; CHARGE PROPERTIES; SOLUTE TRANSPORT; CHLORIDE; MOVEMENT; ADSORPTION; COLUMNS AB Transient unsaturated horizontal column experiments were conducted with a loam soil, under variable boundary conditions, to obtain added insight on nitrate (NO3-) anion exclusion processes. The boundary conditions evaluated were column inlet soil water content (theta(0)), initial soil water content (theta(i)), column inlet soil solution NO3--N concentration (C-0), and the initial soil solution NO3- concentration (C-i). Results consistent with significant anion exclusion of NO3- were exhibited in all experiments. For tests with NO3 solution injected at the column inlet, the soil solution NO3- concentration at the inlet was found to be 12 to 19% less than the injected NO3 concentration. For these same tests, where initial soil conditions were dry with no NO3- originally present, the NO3- concentrations at the wetting front edge were substantially greater than the injected NO3- concentration, while for initially wetter conditions, with or without NO3- originally present, the NO3- front was found to extend well beyond the theoretical "piston front" separating the injected solution from displaced initial soil water. For displacement tests, in which water was injected at the inlet of a column initially wetted with a NO3- solution, the soil solution NO3- front also extended beyond the piston front. Inlet NO3- excluded water content ranged from 0.03 to 0.06 and was not affected by pore water velocity, but did have a modest positive correlation with total water content (r = 0.60 and r = 0.67). The findings of this investigation provide a better overall understanding of nitrate movement through the soil profile. C1 [Allred, Barry J.; Martinez, Luis R.] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Brown, Glenn O.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. RP Allred, BJ (reprint author), USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr,Room 234, Columbus, OH 43210 USA. EM Barry.Allred@ars.usda.gov NR 70 TC 0 Z9 0 U1 4 U2 7 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 742 EP 756 DI 10.2136/sssaj2014.09.0388 PG 15 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000004 ER PT J AU Margenot, AJ Calderon, FJ Bowles, TM Parikh, SJ Jackson, LE AF Margenot, Andrew J. Calderon, Francisco J. Bowles, Timothy M. Parikh, Sanjai J. Jackson, Louise E. TI Soil Organic Matter Functional Group Composition in Relation to Organic Carbon, Nitrogen, and Phosphorus Fractions in Organically Managed Tomato Fields SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID TRANSFORM MIDINFRARED SPECTROSCOPY; PARTICLE-SIZE FRACTIONS; FT-IR SPECTROSCOPY; LONG-TERM; STABILIZATION MECHANISMS; INFRARED-SPECTROSCOPY; QUALITY; AGROECOSYSTEMS; FERTILIZATION; SYSTEMS AB The objectives of this study were to examine soil organic matter (SOM) functional group composition and its relationship to labile SOM fractions with diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). We analyzed soils from 13 organically managed tomato (Solanum lycopersicum) fields in northern California for labile organic C, N, and P fractions and by DRIFTS for bands representing organic functional groups, including aliphatic C-H (2924, 2850, 1470, 1405, 1390 cm(-1)), aromatic C=C (1650 cm(-1)) and C-H (920, 840 cm(-1)), polysaccharide and phenol C-O (1270, 1110, 1080 cm-1), and amine and amide N-H (3400, 1575 cm(-1)). Significant differences in relative band intensities occurred among the 13 organic tomato fields, in particular a relative increase in absorbance of bands representing aliphatic C-H positively associated with soil organic carbon (SOC), as well as permanganate-oxidizable carbon (POXC), extractable organic carbon (EOC) and nitrogen (EON), and potentially mineralizable N (PMN). In comparison, organic P fractions like sodium bicarbonate extractable (NaHCO3-P-o) and sodium hydroxide extractable organic P (NaOH-P-o) were poorly associated with SOC and functional groups represented by bands, including aliphatic C-H. This could reflect limitations of DRIFTS, but is consistent with hypotheses of greater decoupling of C and P vs. C and N in soils. This study implicates relative differences in organic functional groups with differences in SOC and labile SOM fractions, and in agreement with previous studies, identifies absorbance of infrared bands representing aliphatic C-H functional groups in these systems as a potential indicator of SOM transformations related to changes in its labile fractions. C1 [Margenot, Andrew J.; Bowles, Timothy M.; Parikh, Sanjai J.; Jackson, Louise E.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Calderon, Francisco J.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. RP Margenot, AJ (reprint author), Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. EM ajmargenot@ucdavis.edu RI Parikh, Sanjai /F-3476-2011 OI Parikh, Sanjai /0000-0002-5260-0417 FU USDA NIFA Organic Agriculture Research and Education Initiative [2009-01415]; University of California Davis Soils and Biogeochemistry Graduate Group Block Grants FX We thank the eight growers in Yolo County for collaborating on this project, allowing us to sample to their fields and providing management information. Felipe Barrios-Masias, Amanda Hodson, Cristina Lazcano, and Alia Tsang provided field assistance. This research was funded by the USDA NIFA Organic Agriculture Research and Education Initiative Award 2009-01415 and University of California Davis Soils and Biogeochemistry Graduate Group Block Grants. NR 73 TC 6 Z9 6 U1 10 U2 38 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 772 EP 782 DI 10.2136/sssaj2015.02.0070 PG 11 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000007 ER PT J AU Ernakovich, JG Wallenstein, MD Calderon, FJ AF Ernakovich, Jessica G. Wallenstein, Matthew D. Calderon, F. J. TI Chemical Indicators of Cryoturbation and Microbial Processing throughout an Alaskan Permafrost Soil Depth Profile SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER QUALITY; ARCTIC TUNDRA SOILS; DIFFUSE-REFLECTANCE; MIDINFRARED SPECTROSCOPY; CLIMATE-CHANGE; TEMPERATURE SENSITIVITY; ANAEROBIC CONDITIONS; DRIFT SPECTROSCOPY; FTIR SPECTROSCOPY; NORTHERN ALASKA AB Although permafrost soils contain vast stores of organic C, relatively little is known about the chemical composition of their constituent soil organic matter (SOM). Mineral permafrost and organic (OAL) and mineral active layer (MAL) soils from Sagwon Hills, AK were analyzed for total C and N content and SOM chemical composition using Fourier transformed mid-infrared spectroscopy (MidIR). We also investigated techniques for proper collection of MidIR spectra on high C soils, such as permafrost. Principal Components Analysis (PCA) of the MidIR spectra revealed that the OAL was different from the MAL and permafrost based on absorbance of various organic functional groups, such as hydroxyls, alkyls, carbonyls, amines, amides, and esters. The top of the permafrost (0-15 cm below the maximum active layer thaw depth) was also differentiated from the deeper permafrost (16-40 cm below) by the same organic functional groups. Spectral data suggested that there is more chemically labile C (e.g., hydroxyl, amine groups, carbohydrates) in the OAL than the top of the permafrost, which in turn has more labile C than the MAL and deeper permafrost. The chemical similarity between the top of the permafrost and the OAL, and its differences with the MAL, suggest that organic matter (OM) is introduced into the permafrost through cryoturbation. All the soils showed evidence of microbial processing, such as organic acids and carboxylates, however the relative abundance of these compounds varied by soil depth. This study advances our understanding of permafrost C chemistry and the reactivity of constituent compounds. C1 [Ernakovich, Jessica G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Ernakovich, Jessica G.; Wallenstein, Matthew D.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Wallenstein, Matthew D.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [Calderon, F. J.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80520 USA. RP Calderon, FJ (reprint author), USDA ARS, Cent Great Plains Res Stn, Akron, CO 80520 USA. EM Francisco.Calderon@ars.usda.gov RI Wallenstein, Matthew/C-6441-2008; Ernakovich, Jessica/E-4864-2016 OI Wallenstein, Matthew/0000-0002-6219-1442; Ernakovich, Jessica/0000-0002-4493-2489 FU Department of Energy Global Change Education Program; National Science Foundation Graduate Research Fellowship Program; National Science Foundation Office of Polar Programs [0902030]; NSF CAREER award [1255228]; Office of Science (BER), U.S. Department of Energy FX Thank you to Alisa Challenger and Andrew Margenot for help with laboratory analyses, and to the latter for an initial review of this manuscript. Thank you to Mary Stromberger, Rich Conant, Ken Reardon and three anonymous reviewers for their comments during the preparation of this manuscript. Jessica Ernakovich was funded by the Department of Energy Global Change Education Program graduate fellowship during the time of laboratory analyses, and by the National Science Foundation Graduate Research Fellowship Program during data analysis and manuscript preparation. Matthew Wallenstein was supported by award number 0902030 from the National Science Foundation Office of Polar Programs, an NSF CAREER award (#1255228), and a grant from the Office of Science (BER), U.S. Department of Energy. NR 62 TC 3 Z9 3 U1 5 U2 24 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 783 EP 793 DI 10.2136/sssaj2014.10.0420 PG 11 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000008 ER PT J AU Vermang, J Norton, LD Huang, C Cornelis, WM da Silva, AM Gabriels, D AF Vermang, J. Norton, L. D. Huang, C. Cornelis, W. M. da Silva, A. M. Gabriels, D. TI Characterization of Soil Surface Roughness Effects on Runoff and Soil Erosion Rates under Simulated Rainfall SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID MODELING WATER EROSION; OVERLAND-FLOW; AGGREGATE STABILITY; LASER SCANNER; PHYSICAL PRINCIPLES; NET DEPOSITION; TILLAGE; MICROTOPOGRAPHY; INFILTRATION; MICRORELIEF AB This study aims at identifying the influence of soil surface roughness from small to large aggregates (random roughness) on runoff and soil loss and to investigate the interaction with soil surface seal formation. Bulk samples of a silty clay loam soil were sieved to four aggregate-size classes of 3 to 12, 12 to 20, 20 to 45, 45 to 100 mm, and packed in soil trays set at a 5% slope. Rainfall simulations using an oscillating nozzle simulator were conducted for 90 min at an average rainfall intensity of 50.2 mm h(-1). Soil surface roughness was measured using an instantaneous profile laser scanner and surface sealing was studied by macroscopic analysis of epoxy impregnated soil samples. The rainfall simulations revealed longer times to initiate runoff with increasing soil surface roughness. For random roughness levels up to 6 mm, a decrease in final runoff rate with increasing roughness was observed. This can be attributed to a decreased breakdown of the larger roughness elements on rougher surfaces, thus keeping infiltration rate high. For a random roughness larger than 6 mm, a greater final runoff rate was observed. This was caused by the creation of a thick depositional seal in the concentrated flow areas, thus lowering the infiltration rates. Analysis of impregnated soil sample blocks confirmed the formation of a structural surface seal on smooth surfaces, whereas thick depositional seals were visible in the depressional areas of rougher surfaces. Therefore, from our observations it can be learned that soil surface roughness as formed by the presence of different aggregate sizes reduces runoff but that its effect diminishes due to aggregate breakdown and the formation of thick depositional seals in the case of rough soil surfaces. Sediment concentration increased with increasing soil surface roughness, due to runoff concentration in flow paths. Nevertheless, final soil loss rates were comparable for all soil roughness categories, indicating that random roughness is only important in influencing runoff rates and the time to initiate runoff, but not in influencing sediment export through soil loss rates. C1 [Vermang, J.; Cornelis, W. M.; Gabriels, D.] Univ Ghent, Dept Soil Management, UNESCO Chair Eremol, B-9000 Ghent, Belgium. [Norton, L. D.; Huang, C.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [da Silva, A. M.] Campus Expt UNESP, Nucleo Automacao & Tecnol Limpas, Sorocaba, SP, Brazil. RP Vermang, J (reprint author), Univ Ghent, Dept Soil Management, UNESCO Chair Eremol, Coupure Links 653, B-9000 Ghent, Belgium. EM janvermang@gmail.com NR 66 TC 3 Z9 5 U1 6 U2 30 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 903 EP 916 DI 10.2136/sssaj2014.08.0329 PG 14 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000020 ER PT J AU Zhang, XC Zhang, GH Garbrecht, JD Steiner, JL AF Zhang, X. C. Zhang, G. H. Garbrecht, J. D. Steiner, J. L. TI Dating Sediment in a Fast Sedimentation Reservoir using Cesium-137 and Lead-210 SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ACCUMULATION RATES; SOIL-EROSION; WATERSHEDS; OKLAHOMA; TILLAGE; SYSTEMS; CLIMATE; MODELS AB More than 10,000 reservoirs have been constructed in agricultural water-sheds in the United States since the 1940s to control floods and sediments. Reservoir sedimentation records provide a unique opportunity to retrospectively study the effects of land use changes and climate variations on sediment production if the sediment chronology could be properly determined. The objectives of this study were to: (i) evaluate the applicability of four unsupported Pb-210 (Pb-210(ex)) models for dating deposited sediments in fast-sedimentation environments; (ii) estimate the sedimentation rates; and (iii) relate the sedimentation rates to changes in soil conservation measures, land use, and climate. Three sediment profiles were sampled at 5-cm intervals in a flood control reservoir in the Fort Cobb Reservoir Experimental Watershed in southwestern Oklahoma. Radioactivity was measured with a gamma spectrometer for radioisotopes Cs-137 and Pb-210. Four Pb-210(ex) dating models were tested. Three models (constant flux-constant sedimentation [CFCS], constant initial concentration [CIC], and constant rate of supply [CRS]), which are widely used in the lacustrine and marine environments under slow-sedimentation conditions, were not applicable to fast-sedimentation conditions because of considerable Pb-210(ex) input with the sediment, which is assumed negligible in the models. A modified CRS model that uses Cs-137 age markers as references improved determination of the sediment chronology. The average sedimentation rates were three to five times higher during the 1957 to 1963 time period than during the 1964 to 2011 period. The significant sediment reductions coincided with the implementation of numerous conservation measures in the watershed, including check dams, terraces, changing cropping patterns, and progressive adoption of no-till and conservation tillage systems. C1 [Zhang, X. C.; Garbrecht, J. D.; Steiner, J. L.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. [Zhang, G. H.] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. RP Zhang, XC (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM john.zhang@ars.usda.gov FU State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University [2015-KF-09] FX We would like to express our appreciation to Richard L. Lane, planning engineer of the US Department of Agriculture, Natural Resources Conservation Service, for providing the stage-storage curves and the construction and rehabilitation drawing plans of the Cobb Creek No. 2 Dam. Special thanks are also extended to the land owner, Mr. Ron Lowey, for allowing us to take sediment samples from the reservoir. This work was partially supported by State Key Laboratory of Earth Surface Processes and Resource Ecology (2015-KF-09), Beijing Normal University. NR 20 TC 2 Z9 2 U1 4 U2 22 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2015 VL 79 IS 3 BP 948 EP 956 DI 10.2136/sssaj2015.01.0021 PG 9 WC Soil Science SC Agriculture GA CJ6ER UT WOS:000355587000024 ER PT J AU Nicklas, TA O'Neil, CE AF Nicklas, Theresa A. O'Neil, Carol E. TI Development of the SoFAS (Solid Fats and Added Sugars) Concept: The 2010 Dietary Guidelines for Americans SO ADVANCES IN NUTRITION LA English DT Article DE dietary guidelines; public policy; SoFAS; solid fats; added sugars; empty calories ID FOOD GUIDANCE-SYSTEM; NATIONAL-HEALTH; NUTRIENT PROFILES; UNITED-STATES; AWARENESS; PATTERNS; MYPLATE AB The diets of most US children and adults are poor, as reflected by low diet quality scores, when compared with the recommendations of the Dietary Guidelines for Americans (DGAs). Contributing to these low scores is that most Americans overconsume solid fats, which may contain saturated fatty acids and added sugars; although alcohol consumption was generally modest, it provided few nutrients. Thus, the 2005 DGAs generated a new recommendation: to reduce intakes of solid fats, alcohol, and added sugars (SoFAAS). What precipitated the emergence of the new SoFAAS terminology was the concept of discretionary calories (a "calorie" is defined as the amount of energy needed to increase the temperature of 1 kg of water by 1 degrees C), which were defined as calories consumed after an individual had met his or her recommended nutrient intakes while consuming fewer calories than the daily recommendation. A limitation with this concept was that additional amounts of nutrient-dense foods consumed beyond the recommended amount were also considered discretionary calories. The rationale for this was that if nutrient-dense foods were consumed beyond recommended amounts, after total energy intake was met then this constituted excess energy intake. In the 2010 DGAs, the terminology was changed to solid fats and added sugars (SoFAS); thus, alcohol was excluded because it made a minor contribution to overall intake and did not apply to children. The SoFAS terminology also negated nutrient-dense foods that were consumed in amounts above the recommendations for the specific food groups in the food patterns. The ambiguous SoFAS terminology was later changed to "empty calories" to reflect only those calories from solid fats and added sugars (and alcohol if consumed beyond moderate amounts). The purpose of this review is to provide an historical perspective on how the dietary recommendations went from SoFAAS to SoFAS and how discretionary calories went to empty calories between the 2005 and 2010 DGAs. This information will provide practitioners, as well as the public, with valuable information to better understand the evolution of SoFAS over time. C1 [Nicklas, Theresa A.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [O'Neil, Carol E.] Louisiana State Univ, Sch Nutr & Food Sci, Ctr Agr, Baton Rouge, LA 70803 USA. RP Nicklas, TA (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM tnicklas@bcm.edu FU University of Massachusetts; Alliance for Potato Research and Education; USDA/Agriculture Research Service [58-3092-5-001]; USDA Hatch Project [LAB 94209] FX Published in a supplement to Advances in Nutrition. Presented at the University of Massachusetts Roundtable on "Fats and Oils: Where Food Function Meets Health" held in Chicago, IL, 18-19 November 2013. The roundtable was sponsored by the University of Massachusetts and supported by an unrestricted grant from the Alliance for Potato Research and Education. The nonindustry roundtable speakers received travel funding and an honorarium for participation in the meeting and manuscript preparation. The views expressed are those of the authors.; Partial support was received from the USDA/Agriculture Research Service through specific cooperative agreement 58-3092-5-001 and from the USDA Hatch Project LAB 94209. NR 64 TC 0 Z9 0 U1 1 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2015 VL 6 IS 3 BP 368S EP 375S DI 10.3945/an.114.007021 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CJ1JU UT WOS:000355240700020 PM 25979510 ER PT J AU Daigneault, BW McNamara, KA Purdy, PH Krisher, RL Knox, RV Rodriguez-Zas, SL Miller, DJ AF Daigneault, B. W. McNamara, K. A. Purdy, P. H. Krisher, R. L. Knox, R. V. Rodriguez-Zas, S. L. Miller, D. J. TI Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment SO ANDROLOGY LA English DT Article DE cryopreservation; fertilization; in vitro fertilization; sperm function ID IN-VITRO FERTILIZATION; ZONA-BINDING ABILITY; TRIS-BUFFERED MEDIUM; PORCINE OOCYTES; PIG OOCYTES; SUBSEQUENT DEVELOPMENT; INVITRO FERTILIZATION; FETAL PATERNITY; FIELD FERTILITY; FROZEN AB Due to reduced fertility, cryopreserved semen is seldom used for commercial porcine artificial insemination (AI). Predicting the fertility of individual frozen ejaculates for selection of higher quality semen prior to AI would increase overall success. Our objective was to test novel and traditional laboratory analyses to identify characteristics of cryopreserved spermatozoa that are related to boar fertility. Traditional post-thaw analyses of motility, viability, and acrosome integrity were performed on each ejaculate. In vitro fertilization, cleavage, and blastocyst development were also determined. Finally, spermatozoa-oviduct binding and competitive zona-binding assays were applied to assess sperm adhesion to these two matrices. Fertility of the same ejaculates subjected to laboratory assays was determined for each boar by multi-sire AI and defined as (i) the mean percentage of the litter sired and (ii) the mean number of piglets sired in each litter. Means of each laboratory evaluation were calculated for each boar and those values were applied to multiple linear regression analyses to determine which sperm traits could collectively estimate fertility in the simplest model. The regression model to predict the percent of litter sired by each boar was highly effective (p<0.001, r(2)=0.87) and included five traits; acrosome-compromised spermatozoa, percent live spermatozoa (0 and 60min post-thaw), percent total motility, and the number of zona-bound spermatozoa. A second model to predict the number of piglets sired by boar was also effective (p<0.05, r(2)=0.57). These models indicate that the fertility of cryopreserved boar spermatozoa can be predicted effectively by including traditional and novel laboratory assays that consider functions of spermatozoa. C1 [Daigneault, B. W.; McNamara, K. A.; Krisher, R. L.; Knox, R. V.; Rodriguez-Zas, S. L.; Miller, D. J.] Univ Illinois, Dept Anim Sci, Urbana, IL 61821 USA. [Purdy, P. H.] USDA ARS, NCGRP, NAGP, Ft Collins, CO 80522 USA. [Krisher, R. L.] Natl Fdn Fertil Res, Lone Tree, CO USA. RP Miller, DJ (reprint author), Univ Illinois, Dept Anim Sci, 1207 West Gregory Dr, Urbana, IL 61821 USA. EM djmille@illinois.edu OI Miller, David/0000-0001-7147-5546 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2010-85122-20620, 2011-67015-20099] FX Porcine semen was provided by the University of Illinois Swine Research Lab (Champaign, IL), managed by Jonathan Mosely. Ovaries were provided by Momence Packing Co. (Momence, IL). Oviducts were donated by Rantoul Foods (Rantoul, IL). Drs. Elena Silva, Ye Yuan, and Marlon Ocampo provided intellectual and technical support. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2010-85122-20620 from the USDA National Institute of Food and Agriculture to RVK and Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-20099 from the USDA National Institute of Food and Agriculture to DJM. NR 50 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-2919 EI 2047-2927 J9 ANDROLOGY-US JI Andrology PD MAY PY 2015 VL 3 IS 3 BP 558 EP 568 DI 10.1111/andr.12035 PG 11 WC Andrology SC Endocrinology & Metabolism GA CJ2RS UT WOS:000355332800019 PM 25914302 ER PT J AU Doppler, MS Blackwell, BF DeVault, TL Fernandez-Juricic, E AF Doppler, Megan S. Blackwell, Bradley F. DeVault, Travis L. Fernandez-Juricic, Esteban TI Cowbird responses to aircraft with lights tuned to their eyes: Implications for bird-aircraft collisions SO CONDOR LA English DT Article DE aircraft; airports; bird strikes; lighting and wildlife; wildlife-vehicle collisions ID CONE OIL DROPLETS; RISK-ASSESSMENT; VISUAL ECOLOGY; UNITED-STATES; MORTALITY; NOISE; ROAD; PREDATORS; STRIKES; TIME AB Collisions between birds and aircraft (bird strikes) are expensive, risk human lives, and increase bird mortality. Aircraft lighting has been proposed as a potential means of enhancing avian responses to aircraft. Determining the optimal changes to lighting to reduce bird strikes is a complicated problem because avian visual systems differ markedly from that of humans. Icteridae, including Brown-headed Cowbirds (Molothrus ater; hereafter "cowbirds''), are involved in bird strikes, have a well-described visual system, and respond to approaching vehicles and lights. Our goal was to assess cowbirds' responses to a remote-controlled (RC) aircraft fitted with lights tuned to the cowbird eye. On the basis of perceptual modeling (i.e. visual physiology, object and background reflectance, and ambient light conditions), we found that 470-nm lights ("blue'' portion of the human spectrum) would be the most conspicuous wavelength for cowbirds. We used field experiments to examine cowbird response to 470-nm light treatments. Cowbirds exhibited alert behaviors to a stationary RC aircraft with lights on (both continuous and pulsing) in less than half the time they took to do so with lights off. In response to an approaching RC aircraft, cowbird alert responses were delayed at higher aircraft speeds with the lights off, and we noted a less pronounced speed effect with pulsing lights. However, this interaction effect of aircraft speed and lighting was eliminated with continuous lights. Additionally, higher ambient noise levels delayed cowbirds' avoidance responses to the RC aircraft, possibly influencing cowbird behavior as a sensory distractor. We suggest that some types of lighting may enhance the birds' detection and visual tracking of aircraft at high speeds and, thus, holds some potential as a means of reducing the frequency of bird strikes. This sensory-based approach also has implications for management of other bird-object collision problems. C1 [Doppler, Megan S.; Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Blackwell, Bradley F.; DeVault, Travis L.] USDA APHIS WS, Natl Wildlife Res Ctr, Ohio Field Stn, Sandusky, OH USA. RP Fernandez-Juricic, E (reprint author), Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. EM efernan@purdue.edu FU Charles A. and Anne Morrow Lindbergh Foundation; Purdue University; USDA FX Our research was supported by the Charles A. and Anne Morrow Lindbergh Foundation, Purdue University, and the USDA. NR 55 TC 6 Z9 6 U1 4 U2 18 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD MAY PY 2015 VL 117 IS 2 BP 165 EP 177 DI 10.1650/CONDOR-14-157.1 PG 13 WC Ornithology SC Zoology GA CJ2LG UT WOS:000355315500003 ER PT J AU Belaire, JA Westphal, LM Whelan, CJ Minor, ES AF Belaire, J. Amy Westphal, Lynne M. Whelan, Christopher J. Minor, Emily S. TI Urban residents' perceptions of birds in the neighborhood: Biodiversity, cultural ecosystem services, and disservices SO CONDOR LA English DT Article DE Chicago; ecosystem services; residential landscapes; urban bird ID CONSERVATION; LANDSCAPES; DAMAGE; SUSTAINABILITY; MANAGEMENT; FRAMEWORK; BENEFITS; INCREASE; PEOPLE; VALUES AB As our world becomes increasingly urbanized, cities are often where we come into contact with the natural world-not just in parks and urban nature preserves, but in more familiar places like residential yards. We conducted bird surveys and social surveys in Chicago-area residential landscapes near forest preserves (primarily in middle- and high-income areas) to examine residents' perceptions of the birds that co-inhabit their neighborhoods and the relationship of those perceptions with characteristics of the bird community. We found that residents value many aspects of neighborhood birds, especially those related to aesthetics and birds' place in the ecosystem. Our results indicate that while birds were generally well liked and annoyances were minor, several common and visible urban species, such as the House Sparrow (Passer domesticus), European Starling (Sturnus vulgaris), and Blue Jay (Cyanocitta cristata), may attract attention for their negative qualities, such as their sounds and effects on personal property. The results also indicate that residents' valuations of ecosystem services are linked to their perceptions of bird species richness rather than the actual species richness, and people may perceive only a subset of the birds in their neighborhoods. Although birds provide many important ecosystem services, perhaps one of their most important roles in cities is as a relatable and likable connecting point between city dwellers and the broader environment. C1 [Belaire, J. Amy; Minor, Emily S.] Univ Illinois, Dept Biol Sci, Chicago, IL 60680 USA. [Westphal, Lynne M.] US Forest Serv, No Res Stn, People & Their Environm Res Program, Evanston, IL USA. [Whelan, Christopher J.] Univ Illinois, Dept Biol Sci, Illinois Nat Hist Survey, Chicago, IL 60680 USA. RP Belaire, JA (reprint author), St Edwards Univ, Wild Basin Creat Res Ctr, Austin, TX 78704 USA. EM jbelaire@stedwards.edu OI Whelan, Christopher/0000-0001-7511-2603 FU National Science Foundation [DGE-0549245]; University of Illinois at Chicago's Department of Biological Sciences Elmer Hadley Graduate Research Award FX This study is based on work supported by the National Science Foundation (grant DGE-0549245) and by the University of Illinois at Chicago's Department of Biological Sciences Elmer Hadley Graduate Research Award. NR 57 TC 12 Z9 12 U1 18 U2 93 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD MAY PY 2015 VL 117 IS 2 BP 192 EP 202 DI 10.1650/CONDOR-14-128.1 PG 11 WC Ornithology SC Zoology GA CJ2LG UT WOS:000355315500005 ER PT J AU Gaither, CJ Goodrick, S Murphy, BE Poudyal, N AF Gaither, Cassandra Johnson Goodrick, Scott Murphy, Bryn Elise Poudyal, Neelam TI An Exploratory Spatial Analysis of Social Vulnerability and Smoke Plume Dispersion in the U.S. South SO FORESTS LA English DT Article ID WILDLAND-URBAN INTERFACE; AIR-POLLUTION; UNITED-STATES; ASSOCIATION; CALIFORNIA; FIRE AB This study explores the spatial association between social vulnerability and smoke plume dispersion at the census block group level for the 13 southern states in the USDA Forest Service's Region 8. Using environmental justice as a conceptual basis, we use Exploratory Spatial Data Analysis to identify clusters or "hot spots" for the incidence of both higher than average socially marginal populations and plume dispersion. The larger health disparities and environmental justice literature suggests that lower income and minority populations in the U.S. face greater exposure than middle/upper income, non-minority populations to environmental pollutants; however, we are aware of only a few studies examining this relationship in the context of population exposure to wildfires or prescribed fires in the U.S. South, despite the high occurrence of wildfires in the region. Analyses were conducted across five ecoregions in the South and for winter and spring/summer seasons. Results by ecoregion show significant spatial clustering of high social vulnerability block groups in the vicinity of block groups with a high number of smoke plumes (i.e., "hot spots"). Overall, however, socially vulnerable communities are not exposed to more smoke than non-socially vulnerable communities. Data limitations and suggestions for further research are discussed. C1 [Gaither, Cassandra Johnson; Goodrick, Scott] US Forest Serv, USDA, Forestry Sci Lab, Athens, GA 30602 USA. [Murphy, Bryn Elise] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Poudyal, Neelam] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. RP Gaither, CJ (reprint author), US Forest Serv, USDA, Forestry Sci Lab, 320 Green St, Athens, GA 30602 USA. EM cjohnson09@fs.fed.us; sgoodrick@fs.fed.us; bryn.murphy@yale.edu; npoudyal@utk.edu NR 36 TC 2 Z9 2 U1 2 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAY PY 2015 VL 6 IS 5 BP 1397 EP 1421 DI 10.3390/f6051397 PG 25 WC Forestry SC Forestry GA CJ4RJ UT WOS:000355473500001 ER PT J AU Pinchot, CC Clark, SL Schlarbaum, SE Saxton, AM Sung, SJS Hebard, FV AF Pinchot, Cornelia C. Clark, Stacy L. Schlarbaum, Scott E. Saxton, Arnold M. Sung, Shi-Jean S. Hebard, Frederick V. TI Effects of Temporal Dynamics, Nut Weight and Nut Size on Growth of American Chestnut, Chinese Chestnut and Backcross Generations in a Commercial Nursery SO FORESTS LA English DT Article ID NORTHERN RED OAK; ARTIFICIAL REGENERATION; SEEDLING SURVIVAL; CASTANEA-DENTATA; PERFORMANCE; USA; SYSTEM; STATES AB Blight-resistant American chestnut (Castanea dentata) may soon be commercially available, but few studies have tested methods to produce high quality seedlings that will be competitive after planting. This study evaluated the performance of one American, one Chinese (C. mollissima), one second-generation backcross (BC3F2), and 10 third-generation backcross chestnut families (BC3F3). We examine growth over one year in a commercial tree nursery in east Tennessee. We examined relationships among nut size and weight and seedling growth, between germination timing and seedling survival, and between germination percentage and growth. Across the population tested, a 1 g increase in nut weight corresponded to a 6 cm increase in seedling height, a 0.5 mm increase in root collar diameter and one additional first order lateral root, but models had low predictive power. BC3F3 chestnuts grew similarly to American chestnuts, with substantial differences in growth among chestnut families within generation. Nuts that germinated by 23 April had greater than 1955 odds of surviving the first growing season than nuts that germinated in late May. American and backcross chestnut growth slowed in late June, presumably due to exhaustion of their cotyledons before leaf expansion. These results will help nursery managers refine cultural practices to maximize growth of backcross chestnuts. C1 [Pinchot, Cornelia C.] US Forest Serv, USDA, No Res Stn, Delaware, OH 43015 USA. [Clark, Stacy L.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37996 USA. [Schlarbaum, Scott E.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Saxton, Arnold M.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA. [Sung, Shi-Jean S.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Hebard, Frederick V.] Amer Chestnut Fdn, Meadowview, VA 24361 USA. RP Pinchot, CC (reprint author), US Forest Serv, USDA, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM corneliapinchot@fs.fed.us; stacyclark@fs.fed.us; tenntip@utk.edu; asaxton@utk.edu; ssung@fs.fed.us; fred@acf.org FU USDA Forest Service, Northern Research Station, Southern Research Station; University of Tennessee [10-JV-11330134-066]; Forest Service, Southern Research Station [10-JV-11330134-066] FX This work was supported by the USDA Forest Service, Northern Research Station, Southern Research Station; and by a Joint Venture Agreement between the University of Tennessee and the Forest Service, Southern Research Station (10-JV-11330134-066). NR 37 TC 1 Z9 1 U1 4 U2 17 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAY PY 2015 VL 6 IS 5 BP 1537 EP 1556 DI 10.3390/f6051537 PG 20 WC Forestry SC Forestry GA CJ4RJ UT WOS:000355473500008 ER PT J AU Udayanga, D Castlebury, LA Rossman, AY Chukeatirote, E Hyde, KD AF Udayanga, Dhanushka Castlebury, Lisa A. Rossman, Amy Y. Chukeatirote, Ekachai Hyde, Kevin D. TI The Diaporthe sojae species complex: Phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops SO FUNGAL BIOLOGY LA English DT Article DE Genealogical concordance; Glycine max; Phomopsis seed decay; Pod and stem blight; Soft rot of cantaloupe ID PHOMOPSIS SEED DECAY; RENAL-TRANSPLANT RECIPIENT; PHASEOLORUM VAR SOJAE; 1ST REPORT; CARTHAMUS-LANATUS; STEM BLIGHT; MOLECULAR-IDENTIFICATION; MAXIMUM-LIKELIHOOD; FUNGAL PATHOGENS; UNITED-STATES AB Phytopathogenic species of Diaporthe are associated with a number of soybean diseases including seed decay, pod and stem blight and stem canker and lead to considerable crop production losses worldwide. Accurate morphological identification of the species that cause these diseases has been difficult. In this study, we determined the phylogenetic relationships and species boundaries of Diaporthe longicolla, Diaporthe phaseolorum, Diaporthe sojae and closely related taxa. Species boundaries for this complex were determined based on combined phylogenetic analysis of five gene regions: partial sequences of calmodulin (CAL), beta-tubulin (TUB), histone-3 (HIS), translation elongation factor 1-alpha (EF1-alpha), and the nuclear ribosomal internal transcribed spacers (ITS). Phylogenetic analyses revealed that this large complex of taxa is comprised of soybean pathogens as well as species associated with herbaceous field crops and weeds. Diaporthe arctii, Diaporthe batatas, D. phaseolorum and D. sojae are epitypified. The seed decay pathogen D. longicolla was determined to be distinct from D. sojae. D. phaseolorum, originally associated with stem and leaf blight of Lima bean, was not found to be associated with soybean. A new species, Diaporthe ueckerae on Cucumis melo, is introduced with description and illustrations. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Udayanga, Dhanushka; Castlebury, Lisa A.; Rossman, Amy Y.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Udayanga, Dhanushka; Chukeatirote, Ekachai; Hyde, Kevin D.] Mae Fah Luang Univ, Inst Excellence Fungal Res, Chiang Rai 57100, Thailand. [Udayanga, Dhanushka; Chukeatirote, Ekachai; Hyde, Kevin D.] Mae Fah Luang Univ, Sch Sci, Chiang Rai 57100, Thailand. [Hyde, Kevin D.] Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Peoples R China. [Hyde, Kevin D.] World Agroforestry Ctr, East & Cent Asia, Kunming 650201, Peoples R China. RP Castlebury, LA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM Lisa.Castlebury@ars.usda.gov OI Udayanga, Dhanushka/0000-0002-3088-7752 FU U.S. Forest Service International Programs by SMML; Chinese Academy of Sciences [2013T2S0030] FX This work was completed at the Systematic Mycology and Microbiology Laboratory (SMML), Agricultural Research Service, United States Department of Agriculture in Beltsville, MD, USA, under the direction of co-authors Castlebury and Rossman. Dhanushka Udayanga is grateful for the visiting studentship sponsored through the U.S. Forest Service International Programs by SMML. Herbarium curators and managers of BPI, DAOM, FH and NY are thanked for loan specimens. The following individuals contributed to this study by providing cultures and sequence data and we express our appreciation to Nancy G. Fischer, Glen Hartman, Walter Jaklitsch and Alemu Mengistu. KD Hyde thanks The Chinese Academy of Sciences, project number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany. Technical support for this project was provided by Tunesha Phipps whose assistance is greatly appreciated. NR 145 TC 15 Z9 16 U1 1 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD MAY PY 2015 VL 119 IS 5 SI SI BP 383 EP 407 DI 10.1016/j.funbio.2014.10.009 PG 25 WC Mycology SC Mycology GA CI8WW UT WOS:000355053400009 PM 25937066 ER PT J AU Chang, CL Villalun, M Geib, SM Goodman, CL Ringbauer, J Stanley, D AF Chang, Chiou Ling Villalun, MaryAnn Geib, Scott M. Goodman, Cynthia L. Ringbauer, Joseph Stanley, David TI Pupal X-ray irradiation influences protein expression in adults of the oriental fruit fly, Bactrocera dorsalis SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Sterile insect technique; Radiation; Protein expression; Oriental fruit fly Bactrocera dorsalis ID LINE BCIRL-HZAM1 CELLS; OXIDATIVE STRESS; GENE-EXPRESSION; DROSOPHILA; BEHAVIOR AB The oriental fruit fly, Bactrocera dorsalis, is a pest of fruit in the Asia-Pacific region and also, due to quarantine restrictions, a threat to California fruit production. Area-wide suppression of B. dorsalis integrated several approaches including the sterile insect technique (SIT). SIT involves exposing juveniles to gamma radiation and releasing sterile males in substantial numbers, where they successfully compete for wild females. The resulting infertile eggs lead to reduction of the pest populations. Although these protocols are well documented, arising issues about the international transport and distribution of radioactive products is creating difficulties in use of radioactive sources for sterilizing radiation. This led to a shift toward use of X-ray irradiation, which also sterilizes male and female insects. However, use of X-ray technologies is in its infancy and there is virtually no information on the effects of irradiation, other than sterilization, at the physiological and molecular levels of fruit fly biology. We posed the hypothesis that sterilizing male oriental fruit flies via radiation treatment also influences protein expression in the flies. We found that exposing pupae to X-ray irradiation impacted expression of 26 proteins in adult females and 31 proteins in adult males. Seven proteins (glyceraldehyde-3-phosphate dehydrogenase, fructose-bisphosphate aldolase, larval cuticle protein 2, sarcoplasmic calcium-binding protein alpha-B and A chains, general odorant-binding protein 99b, polyubiquitin, and protein disulfide-isomerase) were impacted in both sexes. Some of the proteins act in central energy-generating and in pheromone-signal processing pathways; we infer that males sterilized by X-ray irradiation may be enfeebled in their ability to compete with wild males for females in nature. Published by Elsevier Ltd. C1 [Chang, Chiou Ling; Villalun, MaryAnn; Geib, Scott M.] ARS, USDA, Daniel K Iouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Goodman, Cynthia L.; Ringbauer, Joseph; Stanley, David] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Chang, CL (reprint author), ARS, USDA, Daniel K Iouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM stella.chang@ars.usda.gov OI Geib, Scott/0000-0002-9511-5139 NR 30 TC 2 Z9 2 U1 5 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2015 VL 76 BP 7 EP 16 DI 10.1016/j.jinsphys.2015.03.002 PG 10 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA CJ3CK UT WOS:000355360800002 PM 25772096 ER PT J AU Baumann, F Buck, BJ Metcalf, RV McLaurin, BT Merkler, DJ Carbone, M AF Baumann, Francine Buck, Brenda J. Metcalf, Rodney V. McLaurin, Brett T. Merkler, Douglas J. Carbone, Michele TI The Presence of Asbestos in the Natural Environment is Likely Related to Mesothelioma in Young Individuals and Women from Southern Nevada SO JOURNAL OF THORACIC ONCOLOGY LA English DT Article DE Mesothelioma; Environmental exposure; Asbestos; Lung cancer; Mineral fibers ID ROAD VEHICULAR ACTIVITY; MALIGNANT MESOTHELIOMA; PLEURAL MESOTHELIOMA; UNITED-STATES; RESPIRATORY-DISEASE; VERMICULITE MINERS; NEW-CALEDONIA; EXPOSURE; MORTALITY; ERIONITE AB Background: Inhalation of asbestos and other mineral fibers is known causes of malignant mesothelioma (MM) and lung cancers. In a setting of occupational exposure to asbestos, MM occurs four to eight times more frequently in men than in women, at the median age of 74 years, whereas an environmental exposure to asbestos causes the same number of MMs in men and women, at younger ages. Methods: We studied the geology of Nevada to identify mineral fibers in the environment. We compared MM mortality in different Nevada counties, per sex and age group, for the 1999 to 2010 period. Results: We identified the presence of carcinogenic minerals in Nevada, including actinolite asbestos, erionite, winchite, magnesioriebeckite, and richterite. We discovered that, compared with the United States and other Nevada counties, Clark and Nye counties, in southern Nevada, had a significantly higher proportion of MM that occurred in young individuals (< 55 years) and in women. Conclusions: The elevated percentage of women and individuals younger than 55 years old, combined with a sex ratio of 1: 1 in this age group and the presence of naturally occurring asbestos, suggests that environmental exposure to mineral fibers in southern Nevada may be contributing to some of these mesotheliomas. Further research to assess environmental exposures should allow the development of strategies to minimize exposure, as the development of rural areas continues in Nevada, and to prevent MM and other asbestos-related diseases. C1 [Baumann, Francine; Carbone, Michele] Univ Hawaii, Ctr Canc, Honolulu, HI 96813 USA. [Buck, Brenda J.; Metcalf, Rodney V.] Univ Nevada, Dept Geosci, Las Vegas, NV 89154 USA. [McLaurin, Brett T.] Bloomsburg Univ Penn, Dept Environm Geog & Geol Sci, Bloomsburg, PA 17815 USA. [Merkler, Douglas J.] USDA, Nat Resources Conservat Serv, Las Vegas, NV USA. RP Baumann, F (reprint author), Univ Hawaii, Ctr Canc, 701 Ilalo St, Honolulu, HI 96813 USA. EM fbaumann@cc.hawaii.edu FU National Institute of Health [R01CA106567, P01CA114047, P30CA071789]; University of Hawai'i Foundation FX This work was supported by National Institute of Health (grant numbers R01CA106567, P01CA114047, and P30CA071789) and the University of Hawai'i Foundation, which received donations to support mesothelioma research from Honeywell International Inc., to M. Carbone. NR 43 TC 21 Z9 21 U1 4 U2 14 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1556-0864 EI 1556-1380 J9 J THORAC ONCOL JI J. Thorac. Oncol. PD MAY PY 2015 VL 10 IS 5 BP 731 EP 737 DI 10.1097/JTO.0000000000000506 PG 7 WC Oncology; Respiratory System SC Oncology; Respiratory System GA CI8AP UT WOS:000354990100012 PM 25668121 ER PT J AU Klimentidis, YC Chougule, A Arora, A Frazier-Wood, AC Hsu, CH AF Klimentidis, Yann C. Chougule, Akshay Arora, Amit Frazier-Wood, Alexis C. Hsu, Chiu-Hsieh TI Triglyceride-Increasing Alleles Associated with Protection against Type-2 Diabetes SO PLOS GENETICS LA English DT Article ID GCKR RS780094 POLYMORPHISM; FASTING PLASMA-GLUCOSE; ATHEROSCLEROSIS RISK; HOMA-IR; LOCI; COMMUNITIES; HOMEOSTASIS; POPULATION; OBJECTIVES; DISEASE AB Elevated plasma triglyceride (TG) levels are an established risk factor for type-2 diabetes (T2D). However, recent studies have hinted at the possibility that genetic risk for TG may paradoxically protect against T2D. In this study, we examined the association of genetic risk for TG with incident T2D, and the interaction of baseline TG with TG genetic risk on incident T2D in 13,247 European-Americans (EA) and 3,238 African-Americans (AA) from three prospective cohort studies. A TG genetic risk score (GRS) was calculated based on 31 validated single nucleotide polymorphisms (SNPs). We considered several baseline covariates, including body-mass index (BMI) and lipid traits. Among EA and AA, we find, as expected, that baseline levels of TG are strongly positively associated with incident T2D (p<2 x 10(-10)). However, the TG GRS is negatively associated with T2D (p=0.013), upon adjusting for only race, in the full dataset. Upon additionally adjusting for age, sex, BMI, high-density lipoprotein cholesterol and TG, the TG GRS is significantly and negatively associated with T2D incidence (p=7.0 x 10(-8)), with similar trends among both EA and AA. No single SNP appears to be driving this association. We also find a significant statistical interaction of the TG GRS with TG (p(interaction)=3.3 x 10(-4)), whereby the association of TG with incident T2D is strongest among those with low genetic risk for TG. Further research is needed to understand the likely pleiotropic mechanisms underlying these findings, and to clarify the causal relationship between T2D and TG. C1 [Klimentidis, Yann C.; Chougule, Akshay; Arora, Amit; Hsu, Chiu-Hsieh] Univ Arizona, Mel & Enid Zuckerman Coll Publ Hlth, Div Epidemiol & Biostat, Tucson, AZ 85721 USA. [Frazier-Wood, Alexis C.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. RP Klimentidis, YC (reprint author), Univ Arizona, Mel & Enid Zuckerman Coll Publ Hlth, Div Epidemiol & Biostat, Tucson, AZ 85721 USA. EM yann@email.arizona.edu RI Klimentidis, Yann/B-2348-2015 OI Klimentidis, Yann/0000-0002-6065-4044 FU NIH [K01DK095032]; American Heart Association [14BGIA18740011]; [CRIS 309-5-001-058] FX YCK was supported by NIH grant K01DK095032. ACFW is supported by American Heart Association Grant 14BGIA18740011 and receives USDA support (CRIS 309-5-001-058). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 3 Z9 3 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7390 EI 1553-7404 J9 PLOS GENET JI PLoS Genet. PD MAY PY 2015 VL 11 IS 5 AR UNSP e1005204 DI 10.1371/journal.pgen.1005204 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA CJ2HQ UT WOS:000355305200024 PM 26020539 ER PT J AU Grubaugh, ND Smith, DR Brackney, DE Bosco-Lauth, AM Fauver, JR Campbell, CL Felix, TA Romo, H Duggal, NK Dietrich, EA Eike, T Beane, JE Bowen, RA Black, WC Brault, AC Ebel, GD AF Grubaugh, Nathan D. Smith, Darci R. Brackney, Doug E. Bosco-Lauth, Angela M. Fauver, Joseph R. Campbell, Corey L. Felix, Todd A. Romo, Hannah Duggal, Nisha K. Dietrich, Elizabeth A. Eike, Tyler Beane, Jennifer E. Bowen, Richard A. Black, William C. Brault, Aaron C. Ebel, Gregory D. TI Experimental Evolution of an RNA Virus in Wild Birds: Evidence for Host-Dependent Impacts on Population Structure and Competitive Fitness SO PLOS PATHOGENS LA English DT Article ID WEST-NILE-VIRUS; STRONG PURIFYING SELECTION; NEW-YORK-STATE; ALPHA/BETA-INTERFERON; PHYLOGENETIC ANALYSIS; SEQUENCING DATA; AMERICAN CROWS; HOUSE SPARROWS; DENGUE VIRUS; MOSQUITOS AB Within hosts, RNA viruses form populations that are genetically and phenotypically complex. Heterogeneity in RNA virus genomes arises due to error-prone replication and is reduced by stochastic and selective mechanisms that are incompletely understood. Defining how natural selection shapes RNA virus populations is critical because it can inform treatment paradigms and enhance control efforts. We allowed West Nile virus (WNV) to replicate in wild-caught American crows, house sparrows and American robins to assess how natural selection shapes RNA virus populations in ecologically relevant hosts that differ in susceptibility to virus-induced mortality. After five sequential passages in each bird species, we examined the phenotype and population diversity of WNV through fitness competition assays and next generation sequencing. We demonstrate that fitness gains occur in a species-specific manner, with the greatest replicative fitness gains in robin-passaged WNV and the least in WNV passaged in crows. Sequencing data revealed that intrahost WNV populations were strongly influenced by purifying selection and the overall complexity of the viral populations was similar among passaged hosts. However, the selective pressures that control WNV populations seem to be bird species-dependent. Specifically, crow-passaged WNV populations contained the most unique mutations (similar to 1.7x more than sparrows, similar to 3.4x more than robins) and defective genomes (similar to 1.4x greater than sparrows, similar to 2.7x greater than robins), but the lowest average mutation frequency (about equal to sparrows, similar to 2.6x lower than robins). Therefore, our data suggest that WNV replication in the most disease-susceptible bird species is positively associated with virus mutational tolerance, likely via complementation, and negatively associated with the strength of selection. These differences in genetic composition most likely have distinct phenotypic consequences for the virus populations. Taken together, these results reveal important insights into how different hosts may contribute to the emergence of RNA viruses. C1 [Grubaugh, Nathan D.; Smith, Darci R.; Brackney, Doug E.; Bosco-Lauth, Angela M.; Fauver, Joseph R.; Campbell, Corey L.; Romo, Hannah; Eike, Tyler; Black, William C.; Ebel, Gregory D.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Felix, Todd A.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Lakewood, CO USA. [Romo, Hannah; Duggal, Nisha K.; Dietrich, Elizabeth A.; Brault, Aaron C.] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO USA. [Beane, Jennifer E.] Boston Univ, Sch Med, Sect Computat Biomed, Boston, MA 02118 USA. [Bowen, Richard A.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Biomed Sci, Ft Collins, CO 80523 USA. RP Grubaugh, ND (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. EM gregory.ebel@colostate.edu RI Ebel, Gregory/D-8324-2017; OI Beane-Ebel, Jennifer/0000-0002-6699-2132 FU National Institute of Allergy and Infectious Disease, National Institutes of Health [AI067380] FX This work was funded by a grant from the National Institute of Allergy and Infectious Disease, National Institutes of Health under grant number AI067380 (GDE). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 56 TC 10 Z9 10 U1 4 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD MAY PY 2015 VL 11 IS 5 AR e1004874 DI 10.1371/journal.ppat.1004874 PG 19 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA CJ1UF UT WOS:000355269300025 PM 25993022 ER PT J AU Liu, MF Lin, L Gebremariam, T Luo, GPS Skory, CD French, SW Chou, TF Edwards, JE Ibrahim, AS AF Liu, Mingfu Lin, Lin Gebremariam, Teclegiorgis Luo, Guanpingsheng Skory, Christopher D. French, Samuel W. Chou, Tsui-Fen Edwards, John E., Jr. Ibrahim, Ashraf S. TI Fob1 and Fob2 Proteins Are Virulence Determinants of Rhizopus oryzae via Facilitating Iron Uptake from Ferrioxamine SO PLOS PATHOGENS LA English DT Article ID SACCHAROMYCES-CEREVISIAE; IMPROVES SURVIVAL; INVASIVE ZYGOMYCOSIS; DESFERRIOXAMINE-B; DIALYSIS PATIENTS; IN-VITRO; MUCORMYCOSIS; SIDEROPHORE; TRANSPORT; MECHANISM AB Dialysis patients with chronic renal failure receiving deferoxamine for treating iron overload are uniquely predisposed for mucormycosis, which is most often caused by Rhizopus oryzae. Although the deferoxamine siderophore is not secreted by Mucorales, previous studies established that Rhizopus species utilize iron from ferrioxamine (iron-rich form of deferoxamine). Here we determined that the CBS domain proteins of Fob1 and Fob2 act as receptors on the cell surface of R. oryzae during iron uptake from ferrioxamine. Fob1 and Fob2 cell surface expression was induced in the presence of ferrioxamine and bound radiolabeled ferrioxamine. A R. oryzae strain with targeted reduced Fob1/Fob2 expression was impaired for iron uptake, germinating, and growing on medium with ferrioxamine as the sole source of iron. This strain also exhibited reduced virulence in a deferoxamine-treated, but not the diabetic ketoacidotic (DKA), mouse model of mucormycosis. The mechanism by which R. oryzae obtains iron from ferrioxamine involves the reductase/permease uptake system since the growth on ferrioxamine supplemented medium is associated with elevated reductase activity and the use of the ferrous chelator bathophenanthroline disulfonate abrogates iron uptake and growth on medium supplemented with ferrioxamine as a sole source of iron. Finally, R. oryzae mutants with reduced copies of the high affinity iron permease (FTR1) or with decreased FTR1 expression had an impaired iron uptake from ferrioxamine in vitro and reduced virulence in the deferoxamine-treated mouse model of mucormycosis. These two receptors appear to be conserved in Mucorales, and can be the subject of future novel therapy to maintain the use of deferoxamine for treating iron-overload. C1 [Liu, Mingfu; Lin, Lin; Gebremariam, Teclegiorgis; Luo, Guanpingsheng; French, Samuel W.; Edwards, John E., Jr.; Ibrahim, Ashraf S.] Harbor Univ Calif, Los Angeles Biomed Res Inst, Div Infect Dis, Los Angeles UCLA Med Ctr, Torrance, CA 90501 USA. [Lin, Lin; Edwards, John E., Jr.; Ibrahim, Ashraf S.] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA. [Skory, Christopher D.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [French, Samuel W.] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol, Los Angeles, CA 90095 USA. [French, Samuel W.] Harbor Univ Calif, Div Med Genet, Dept Pediat, Los Angeles Med Ctr, Torrance, CA USA. [Chou, Tsui-Fen] Harbor Univ Calif, Div Med Genet, Dept Pediat, Los Angeles Med Ctr, Torrance, CA USA. [Chou, Tsui-Fen] Los Angeles Biomed Res Inst, Torrance, CA USA. RP Ibrahim, AS (reprint author), Harbor Univ Calif, Los Angeles Biomed Res Inst, Div Infect Dis, Los Angeles UCLA Med Ctr, Torrance, CA 90501 USA. EM ibrahim@labiomed.org FU Public Health Service [R01 AI063503] FX This work was supported by Public Health Service grants R01 AI063503 to ASI. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 67 TC 2 Z9 2 U1 2 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD MAY PY 2015 VL 11 IS 5 AR UNSP e1004842 DI 10.1371/journal.ppat.1004842 PG 33 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA CJ1UF UT WOS:000355269300016 PM 25974051 ER PT J AU Krochak, P Zhu, JY AF Krochak, Paul Zhu, J. Y. TI Highlighting the role of fluid mechanics SO TAPPI JOURNAL LA English DT Editorial Material C1 [Krochak, Paul] Innventia AB, Stockholm, Sweden. [Zhu, J. Y.] USDA, Forest Serv, Forest Prod Lab, Madison, WI USA. RP Krochak, P (reprint author), Innventia AB, Stockholm, Sweden. EM paul.krochak@innventia.com; jzhu@fs.fed.us NR 0 TC 0 Z9 0 U1 1 U2 3 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD MAY PY 2015 VL 14 IS 5 BP 289 EP 289 PG 1 WC Materials Science, Paper & Wood SC Materials Science GA CJ2OF UT WOS:000355323600001 ER PT J AU O'Neel, S Hood, E Bidlack, AL Fleming, SW Arimitsu, ML Arendt, A Burgess, E Sergeant, CJ Beaudreau, AH Timm, K Hayward, GD Reynolds, JH Pyare, S AF O'Neel, Shad Hood, Eran Bidlack, Allison L. Fleming, Sean W. Arimitsu, Mayumi L. Arendt, Anthony Burgess, Evan Sergeant, Christopher J. Beaudreau, Anne H. Timm, Kristin Hayward, Gregory D. Reynolds, Joel H. Pyare, Sanjay TI lcefield-to-Ocean Linkages across the Northern Pacific Coastal Temperate Rainforest Ecosystem SO BIOSCIENCE LA English DT Article DE ecosystem response; climate change; glacier change; biogeochemistry; hydrology; marine ecology ID DISSOLVED ORGANIC-MATTER; BAY-NATIONAL-PARK; SEA-LEVEL RISE; GLACIER-BAY; SOUTHEAST ALASKA; TIDEWATER GLACIERS; CLIMATE-CHANGE; REGIME SHIFTS; CHLOROPHYLL-A; GULF AB Rates of glacier mass loss in the northern Pacific coastal temperate rainforest (PCTR) are among the highest on Earth, and changes in glacier volume and extent will affect the flow regime and chemistry of coastal rivers, as well as the nearshore marine ecosystem of the Gulf of Alaska. Here we synthesize physical, chemical and biological linkages that characterize the northern PCTR ecosystem, with particular emphasis on the potential impacts of glacier change in the coastal mountain ranges on the surface-water hydrology, biogeochemistry, coastal oceanography and aquatic ecology. We also evaluate the relative importance and interplay between interannual variability and long-term trends in key physical drivers and ecological responses. To advance our knowledge of the northern PCTR, we advocate for cross-disciplinary research bridging the icefield-to-ocean ecosystem that can be paired with long-term scientific records and designed to inform decisionmakers. C1 [O'Neel, Shad; Arimitsu, Mayumi L.; Burgess, Evan] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Hood, Eran; Bidlack, Allison L.; Pyare, Sanjay] Univ Alaska Southeast, Dept Nat Sci, Juneau, AK USA. Univ Alaska Southeast, Alaska Coastal Rainforest Ctr, Juneau, AK USA. [Fleming, Sean W.] Environm Canada, Meteorol Serv Canada, Div Sci, Vancouver, BC, Canada. [Arendt, Anthony; Burgess, Evan] Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK USA. [Sergeant, Christopher J.] Southeast Alaska Inventory & Monitoring Network, Natl Pk Serv, Juneau, AK USA. [Arimitsu, Mayumi L.; Beaudreau, Anne H.] Univ Alaska Fairbanks, Sch Fisheries & Ocean Sci, Juneau, AK USA. [Timm, Kristin] Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, Juneau, AK USA. [Hayward, Gregory D.] US Forest Serv, Anchorage, AK USA. [Reynolds, Joel H.] Western Alaska Landscape Conservat Cooperat, Anchorage, AK USA. RP O'Neel, S (reprint author), US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. FU Department of Interior, Alaska Climate Science Center; US Geological Survey (USGS) Climate; Land Use Change Mission; US National Science Foundation [OIA-1208927, DEB-1145885] FX This article resulted from a workshop on glacier change in the PCTR that was funded by the Department of Interior, Alaska Climate Science Center and attended by all of the authors. Additional support for this work also came from the US Geological Survey (USGS) Climate and Land Use Change Mission and the US National Science Foundation (through grants no. OIA-1208927 and no. DEB-1145885). Reviews from Christian Zimmerman, Vanessa von Biela, Sandy Milner and anonymous reviewers helped improve the focus and clarity of the manuscript. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of any government agencies. NR 77 TC 9 Z9 9 U1 4 U2 20 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD MAY PY 2015 VL 65 IS 5 BP 499 EP 512 DI 10.1093/biosci/biv027 PG 14 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CI4XD UT WOS:000354756300008 ER PT J AU Jordan, NR Davis, AS AF Jordan, Nicholas R. Davis, Adam S. TI Middle-Way Strategies for Sustainable Intensification of Agriculture SO BIOSCIENCE LA English DT Article DE sustainable development; sustainable agriculture; transdisciplinary; agroecology; innovation ID CROPPING SYSTEMS; GOVERNANCE; MANAGEMENT; PARADIGM; LAND AB Sustainable intensification is a widely shared idealistic vision for agriculture, in which production and other ecosystem services jointly increase to meet the future needs of humanity and the biosphere. Realizing this vision will require an outcome-driven approach that draws on all available practices and technologies to design agroecosystems that negotiate the difficult trade-offs associated with reconciling sustainability along production, economic, and environmental performance dimensions. To create such "middle-way" strategies for sustainable intensification, we call for strongly transdisciplinary research systems that coordinate integrative, research among major streams of agriculture via ethical and philosophical orientation provided by "purposive disciplines," such as applied ethics and design. Middle-way research partnerships can be strengthened by linking outcomes to mutually agreeable goals, such as "net agroecosystem aggradation." We illustrate our proposals by outlining a potential middle-way strategy for weed management, describing its ethical rationale, and suggesting a research policy agenda for sustainable intensification via middle-way agriculture. C1 [Jordan, Nicholas R.] Univ Minnesota, Agron & Plant Genet Dept, St Paul, MN 55108 USA. [Davis, Adam S.] USDA ARS, Global Change & Photosynthesis Res Unit, Washington, DC 20250 USA. [Davis, Adam S.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Jordan, NR (reprint author), Univ Minnesota, Agron & Plant Genet Dept, St Paul, MN 55108 USA. EM jorda020@umn.edu; asdavisl@illinois.edu NR 42 TC 5 Z9 5 U1 9 U2 58 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD MAY PY 2015 VL 65 IS 5 BP 513 EP 519 DI 10.1093/biosci/biv033 PG 7 WC Biology SC Life Sciences & Biomedicine - Other Topics GA CI4XD UT WOS:000354756300009 ER PT J AU de Lima, FG Lee, ST Pfister, JA Miyagi, ES Costa, GL da Silva, RD Fioravanti, MCS AF de Lima, Flavia Gontijo Lee, Stephen Thomas Pfister, James Alan Miyagi, Eliane Sayuri Costa, Gustavo Lage da Silva, Roberta Dias Soares Fioravanti, Maria Clorinda TI The effect of ensiling and haymaking on the concentrations of steroidal saponin in two Brachiaria grass species SO CIENCIA RURAL LA English DT Article DE haymaking; hepatogenous photosensitization; saponin; protodioscin; signalgrass; silage ID SPORIDESMIN PRODUCTION; PITHOMYCES AB Brachiaria (signalgrass) is now the most widely used tropical grass genus in Central and South America. However, Brachiaria spp. can cause hepatogenous photosensitization in livestock. Steroidal saponins, specifically protodioscin, present in Brachiaria spp. may be responsible for liver injury and subsequent photosensitization. The objective of this study was to determine the effect of ensiling Brachiaria decumbens and Brachiaria brizantha or making hay from Brachiaria decumbens on the concentrations of steroidal saponin in these grasses. Brachiaria grass had no detectable levels of the saponin protodioscin after 24 days of ensiling. In addition, in Brachiaria decumbens, the concentration of the protodioscin decreased 48% over the first three days after haymaking and then remained constant. These results suggest that livestock consuming Brachiaria either as silage or hay may have reduced risk of intoxication by protodioscin. C1 [de Lima, Flavia Gontijo; Miyagi, Eliane Sayuri; Costa, Gustavo Lage; da Silva, Roberta Dias; Soares Fioravanti, Maria Clorinda] Univ Fed Goias, BR-74001970 Goiania, Go, Brazil. [Lee, Stephen Thomas; Pfister, James Alan] USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP de Lima, FG (reprint author), Univ Fed Goias, BR-74001970 Goiania, Go, Brazil. EM flaviamedvet@yahoo.com.br FU Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES); Brazilian National Council for Scientific and Technological Development (CNPq) FX This study was made possible by grants from the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) and the Brazilian National Council for Scientific and Technological Development (CNPq). The authors wish to thank Tayrone F. Prado, Weyguer C. Fernandes, Helton F. Oliveira and Sharon Azzopardi for their technical support. NR 24 TC 1 Z9 1 U1 1 U2 6 PU UNIV FEDERAL SANTA MARIA PI SANTA MARIA PA UNIV FEDERAL SANTA MARIA, SANTA MARIA, RS 97105.9, BRAZIL SN 0103-8478 EI 1678-4596 J9 CIENC RURAL JI Cienc. Rural PD MAY PY 2015 VL 45 IS 5 BP 858 EP 863 DI 10.1590/0103-8478cr20141305 PG 6 WC Agronomy SC Agriculture GA CI5IO UT WOS:000354789100017 ER PT J AU Chikaraishi, Y Steffan, SA Takano, Y Ohkouchi, N AF Chikaraishi, Yoshito Steffan, Shawn A. Takano, Yoshinori Ohkouchi, Naohiko TI Diet quality influences isotopic discrimination among amino acids in an aquatic vertebrate SO ECOLOGY AND EVOLUTION LA English DT Article DE Amino acids; diet quality; food web; nitrogen isotopic composition; trophic discrimination factor ID TROPHIC POSITION; FOOD WEBS; NITROGEN; FRACTIONATION; DELTA-N-15; ENRICHMENT; PLANKTON; ECOLOGY AB Stable nitrogen isotopic composition of amino acids (N-15(AA)) has recently been employed as a powerful tool in ecological food web studies, particularly for estimating the trophic position (TP) of animal species in food webs. However, the validity of these estimates depends on the consistency of the trophic discrimination factor (TDF; = N-15(AA) at each shift of trophic level) among a suite of amino acids within the tissues of consumer species. In this study, we determined the TDF values of amino acids in tadpoles (the Japanese toad, Bufo japonicus) reared exclusively on one of three diets that differed in nutritional quality. The diets were commercial fish-food pellets (plant and animal biomass), bloodworms (animal biomass), and boiled white rice (plant carbohydrate), representing a balanced, protein-rich, and protein-poor diet, respectively. The TDF values of two source amino acids (Src-AAs), methionine and phenylalanine, were close to zero (0.3-0.5 parts per thousand) among the three diets, typifying the values reported in the literature (similar to 0.5 parts per thousand and similar to 0.4 parts per thousand, respectively). However, TDF values of trophic amino acids (Tr-AAs) including alanine, valine, leucine, isoleucine, and glutamic acid varied by diet: for example, the glutamic acid TDF was similar to the standard value (similar to 8.0 parts per thousand) when tadpoles were fed either the commercial pellets (8.0 parts per thousand) or bloodworms (7.9 parts per thousand), but when they were fed boiled rice, the TDF was significantly reduced (0.6 parts per thousand). These results suggest that a profound lack of dietary protein may alter the TDF values of glutamic acid (and other Tr-AAs and glycine) within consumer species, but not the two Src-AAs (i.e., methionine and phenylalanine). Knowledge of how a nutritionally poor diet can influence the TDF of Tr- and Src-AAs will allow amino acid isotopic analyses to better estimate TP among free-roaming animals. C1 [Chikaraishi, Yoshito; Takano, Yoshinori; Ohkouchi, Naohiko] Japan Agcy Marine Earth Sci & Technol, Yokosuka, Kanagawa 2370061, Japan. [Steffan, Shawn A.] Univ Wisconsin, Dept Entomol, Vegetable Crops Res Unit, USDA,ARS, Madison, WI 53706 USA. RP Chikaraishi, Y (reprint author), Japan Agcy Marine Earth Sci & Technol, 2-15 Natsushima Cho, Yokosuka, Kanagawa 2370061, Japan. EM ychikaraishi@jamstec.go.jp FU JSPS; USDA-ARS FX This work was supported by Grant-in-Aid for Scientific Research of the JSPS to YC, YT, and NO, and USDA-ARS appropriated funds to SAS. NR 34 TC 6 Z9 6 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD MAY PY 2015 VL 5 IS 10 BP 2048 EP 2059 DI 10.1002/ece3.1491 PG 12 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA CI5QC UT WOS:000354812500010 PM 26045955 ER PT J AU Ross, DS Bailey, SW Briggs, RD Curry, J Fernandez, IJ Fredriksen, G Goodale, CL Hazlett, PW Heine, PR Johnson, CE Larson, JT Lawrence, GB Kolka, RK Ouimet, R Pare, D Richter, DD Schirmer, CD Warby, RA AF Ross, D. S. Bailey, S. W. Briggs, R. D. Curry, J. Fernandez, I. J. Fredriksen, G. Goodale, C. L. Hazlett, P. W. Heine, P. R. Johnson, C. E. Larson, J. T. Lawrence, G. B. Kolka, R. K. Ouimet, R. Pare, D. Richter, D. deB. Schirmer, C. D. Warby, R. A. TI Inter-laboratory variation in the chemical analysis of acidic forest soil reference samples from eastern North America SO ECOSPHERE LA English DT Article DE aluminum; calcium; carbon; forest soils; inter-laboratory variability; laboratory methods; pH; reference soils; soil analysis; Special Feature: Uncertainty Analysis; uncertainty ID NORTHEASTERN UNITED-STATES; RED SPRUCE FORESTS; SUGAR MAPLE; EXCHANGEABLE HYDROGEN; ALLEGHENY PLATEAU; CATION-EXCHANGE; ORGANIC SOILS; CALCIUM; CARBON; PH AB Long-term forest soil monitoring and research often requires a comparison of laboratory data generated at different times and in different laboratories. Quantifying the uncertainty associated with these analyses is necessary to assess temporal changes in soil properties. Forest soil chemical properties, and methods to measure these properties, often differ from agronomic and horticultural soils. Soil proficiency programs do not generally include forest soil samples that are highly acidic, high in extractable Al, low in extractable Ca and often high in carbon. To determine the uncertainty associated with specific analytical methods for forest soils, we collected and distributed samples from two soil horizons (Oa and Bs) to 15 laboratories in the eastern United States and Canada. Soil properties measured included total organic carbon and nitrogen, pH and exchangeable cations. Overall, results were consistent despite some differences in methodology. We calculated the median absolute deviation (MAD) for each measurement and considered the acceptable range to be the median +/- 2.5 x MAD. Variability among laboratories was usually as low as the typical variability within a laboratory. A few areas of concern include a lack of consistency in the measurement and expression of results on a dry weight basis, relatively high variability in the C/N ratio in the Bs horizon, challenges associated with determining exchangeable cations at concentrations near the lower reporting range of some laboratories and the operationally defined nature of aluminum extractability. Recommendations include a continuation of reference forest soil exchange programs to quantify the uncertainty associated with these analyses in conjunction with ongoing efforts to review and standardize laboratory methods. C1 [Ross, D. S.] Univ Vermont, Dept Plant & Soil Sci, Burlington, VT 05405 USA. [Bailey, S. W.] USDA Forest Serv, No Res Stn, Hubbard Brook Expt Forest, North Woodstock, NH 03262 USA. [Briggs, R. D.; Schirmer, C. D.] SUNY Coll Environm Sci & Forestry, Div Environm Sci, Syracuse, NY 13210 USA. [Curry, J.; Hazlett, P. W.] Nat Resources Canada, Canadian Forest Serv, Sault Ste Marie, ON 6A 2E5, Canada. [Fernandez, I. J.] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Fredriksen, G.; Goodale, C. L.] Cornell Univ, Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. [Heine, P. R.; Richter, D. deB.] Duke Univ, Environm Sci & Policy Div, Nicholas Sch Environm, Durham, NC 27708 USA. [Johnson, C. E.] Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. [Larson, J. T.; Kolka, R. K.] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Lawrence, G. B.] US Geol Survey, New York Water Sci Ctr, Troy, NY 12180 USA. [Ouimet, R.] Minist Foret Faune & Parcs Quebec, Direct Rech Forestiere, Quebec City, PQ G1P 3W8, Canada. [Pare, D.] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada. [Warby, R. A.] Warby Grp LLC, North Attleboro, MA 02760 USA. RP Ross, DS (reprint author), Univ Vermont, Dept Plant & Soil Sci, Burlington, VT 05405 USA. EM dross@uvm.edu RI Ross, Donald/A-4477-2008; Johnson, Chris/A-6979-2011; OI Ross, Donald/0000-0002-5390-6602; Johnson, Chris/0000-0001-9079-813X; Bailey, Scott/0000-0002-9160-156X NR 58 TC 3 Z9 3 U1 3 U2 13 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2015 VL 6 IS 5 AR 73 DI 10.1890/ES14-00209.1 PG 22 WC Ecology SC Environmental Sciences & Ecology GA CI5FA UT WOS:000354777800002 ER PT J AU Stephens, SL Lydersen, JM Collins, BM Fry, DL Meyer, MD AF Stephens, Scott L. Lydersen, Jamie M. Collins, Brandon M. Fry, Danny L. Meyer, Marc D. TI Historical and current landscape-scale ponderosa pine and mixed conifer forest structure in the Southern Sierra Nevada SO ECOSPHERE LA English DT Article DE fire ecology; fire management; fire severity; forest ecology; forest resiliency; reference conditions; restoration ID YOSEMITE-NATIONAL-PARK; SPATIAL-PATTERNS; FIRE SEVERITY; RESTORATION TREATMENTS; FUEL REDUCTION; UNITED-STATES; WOODY DEBRIS; CALIFORNIA; USA; STAND AB Many managers today are tasked with restoring forests to mitigate the potential for uncharacteristically severe fire. One challenge to this mandate is the lack of large-scale reference information on forest structure prior to impacts from Euro-American settlement. We used a robust 1911 historical dataset that covers a large geographic extent (>10,000 ha) and has unbiased sampling locations to compare past and current forest conditions for ponderosa pine and mixed conifer forests in the southern Sierra Nevada. The 1911 dataset contained records from 18,052 trees in 378 sampled transects, totaling just over 300 ha in transect area. Forest structure was highly variable in 1911 and shrubs were found in 54% of transects. Total tree basal area ranged from 1 to 60 m(2) ha(-1) and tree density from 2 to 170 ha(-1) (based on trees >30 cm dbh). K-means cluster analysis divided transects into four groups: mixed conifer-high basal area (MC High BA), mixed conifer-average basal area (MC Ave BA), mixed conifer-average basal area-high shrubs (MC Ave BA Shrubs), and ponderosa pine (Pond Pine). The percentage of this 1911 landscape that experienced high severity fire was low and varied from 1-3% in mixed conifer forests and 4-6% in ponderosa pine forests. Comparing forest inventory data from 1911 to the present indicates that current forests have changed drastically, particularly in tree density, canopy cover, the density of large trees, dominance of white fir in mixed conifer forests, and the similarity of tree basal area in contemporary ponderosa pine and mixed conifer forests. Average forest canopy cover increased from 25-49% in mixed conifer forests, and from 12-49% in ponderosa pine forests from 1911 to the present; canopy cover in current forest types is similar but in 1911 mixed conifer forests had twice the canopy cover as ponderosa pine forests. Current forest restoration goals in the southern Sierra Nevada are often skewed toward the higher range of these historical values, which will limit the effectiveness of these treatments if the objective is to produce resilient forest ecosystems into the future. C1 [Stephens, Scott L.; Fry, Danny L.] Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Lydersen, Jamie M.; Collins, Brandon M.] USDA Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Coll Nat Resources, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. [Meyer, Marc D.] USDA Forest Serv, Pacific Southwest Reg, Clovis, CA 93611 USA. RP Stephens, SL (reprint author), Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. EM sstephens@berkeley.edu FU University of California Agriculture and Natural Resources Division; USFS Pacific Southwest Research Station FX We thank Jim Baldwin for advice on statistical approaches. We appreciate the assistance from Emily Fales and Keith Arnold in entering the 1911 information into a database. We thank USFS District Archaeologist Erin Potter and Fuels Officer Burt Stalter for providing the historic forest photos. We thank Carlos Ramirez and Deb Beardsley for producing the FIA data used in this study. Funding was provided by grants from the University of California Agriculture and Natural Resources Division and USFS Pacific Southwest Research Station. NR 91 TC 17 Z9 17 U1 6 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2015 VL 6 IS 5 AR 79 DI 10.1890/ES14-00379.1 PG 63 WC Ecology SC Environmental Sciences & Ecology GA CI5FA UT WOS:000354777800008 ER PT J AU Kliment, LK Rokhsaz, K Nelson, J Terning, B Weinstein, EM AF Kliment, Linda K. Rokhsaz, Kamran Nelson, John Terning, Brett Weinstein, Edward M. TI Usage and Flight Loads Analysis of King Airs in Aerial Firefighting Missions SO JOURNAL OF AIRCRAFT LA English DT Article AB Flight data of Beechcraft King Airs used as aerial supervision modules and lead aircraft by the U.S. Forest Service are analyzed. The operational conditions experienced by the airplanes are studied as well as the magnitude of the vertical loads. The airplanes are flown in four different types of missions in U.S. Forest Service operation. Data from 1853 flights and seven aircraft are presented. Spectra are shown for gust and maneuver loads, separated using the two-second rule. These are given for the entire flight for various mean sea-level altitudes. In addition, the combined loads are presented and compared to existing data and Federal Aviation Regulation 23. It is shown that these aircraft are operated well within their design envelope. It is demonstrated that, although gust loads are comparable to those outlined in Federal Aviation Regulation 23, the maneuver load frequencies far exceed those of typical airframe use. Although these results do not warrant changes to Federal Aviation Regulation 23, they highlight the need for modified airframe maintenance and inspection procedures as well as the estimated airframe fatigue life. C1 [Kliment, Linda K.; Rokhsaz, Kamran] Wichita State Univ, Dept Aerosp Engn, Wichita, KS 67260 USA. [Nelson, John] US Forest Serv, Washington Off West, Fire & Aviat Management, Boise, ID 83705 USA. [Terning, Brett] US Forest Serv, Washington Off West, Boise, ID 83705 USA. [Weinstein, Edward M.] FAA, William J Hughes Tech Center, Atlantic City, NJ 08405 USA. RP Kliment, LK (reprint author), Wichita State Univ, Dept Aerosp Engn, Wichita, KS 67260 USA. FU Federal Aviation Administration [08-G-015] FX This work was partially funded by the Federal Aviation Administration under the grant 08-G-015. The authors would like to acknowledge the technical support provided by HBM nCode and the U.S. Forest Service. NR 11 TC 0 Z9 0 U1 1 U2 6 PU AMER INST AERONAUTICS ASTRONAUTICS PI RESTON PA 1801 ALEXANDER BELL DRIVE, STE 500, RESTON, VA 22091-4344 USA SN 0021-8669 EI 1533-3868 J9 J AIRCRAFT JI J. Aircr. PD MAY-JUN PY 2015 VL 52 IS 3 BP 910 EP 916 DI 10.2514/1.C032877 PG 7 WC Engineering, Aerospace SC Engineering GA CI4VH UT WOS:000354751400019 ER PT J AU Walter, S Kahla, A Arunachalam, C Perochon, A Khan, MR Scofield, SR Doohan, FM AF Walter, Stephanie Kahla, Amal Arunachalam, Chanemoughasoundharam Perochon, Alexandre Khan, Mojibur R. Scofield, Steven R. Doohan, Fiona M. TI A wheat ABC transporter contributes to both grain formation and mycotoxin tolerance SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE ABC transporter; breeding; deoxynivalenol; Fusarium head blight; grain formation; multidrug resistance; ripening; toxin resistance; wheat ID HEAD BLIGHT RESISTANCE; QUANTITATIVE TRAIT LOCI; MULTIPLE FUNGAL PATHOGENS; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; UDP-GLUCOSYLTRANSFERASE; TRANSCRIPTOME ANALYSIS; DEPENDENT TRANSPORT; HEXAPLOID WHEAT; SPRING WHEAT AB Genes that enhance resistance to the Fusarium virulence factor deoxynivalenol (DON) are targets for disease resistance breeding. This study provides direct evidence that a wheat ABCC3 enhances resistance to DON.The mycotoxin deoxynivalenol (DON) acts as a disease virulence factor for Fusarium fungi, and tolerance of DON enhances wheat resistance to Fusarium head blight (FHB) disease. Two variants of an ATP-binding cassette (ABC) family C transporter gene were cloned from DON-treated wheat mRNA, namely TaABCC3.1 and TaABCC3.2. These represent two of three putative genes identified on chromosomes 3A, 3B, and 3D of the wheat genome sequence. Variant TaABCC3.1 represents the DON-responsive transcript previously associated with DON resistance in wheat. PCR-based mapping and in silico sequence analyses located TaABCC3.1 to the short arm of wheat chromosome 3B (not within the FHB resistance quantitative trait locus Fhb1). In silico analyses of microarray data indicated that TaABCC3 genes are expressed in reproductive tissue and roots, and in response to the DON producer Fusarium graminearum. Gene expression studies showed that TaABCC3.1 is activated as part of the early host response to DON and in response to the FHB defence hormone jasmonic acid. Virus-induced gene silencing (VIGS) confirmed that TaABCC3 genes contributed to DON tolerance. VIGS was performed using two independent viral construct applications: one specifically targeted TaABCC3.1 for silencing, while the other targeted this gene and the chromosome 3A homeologue. In both instances, VIGS resulted in more toxin-induced discoloration of spikelets, compared with the DON effects in non-silenced spikelets at 14 d after toxin treatment (a parts per thousand yen2.2-fold increase, P < 0.05). Silencing by both VIGS constructs enhanced head ripening, and especially so in DON-treated heads. VIGS of TaABCC3 genes also reduced the grain number by more than 28% (P < 0.05), both with and without DON treatment, and the effects were greater for the construct that targeted the two homeologues. Hence, DON-responsive TaABCC3 genes warrant further study to determine their potential as disease resistance breeding targets and their function in grain formation and ripening. C1 [Walter, Stephanie; Kahla, Amal; Arunachalam, Chanemoughasoundharam; Perochon, Alexandre; Khan, Mojibur R.; Doohan, Fiona M.] Natl Univ Ireland Univ Coll Dublin, UCD Earth Inst, Sci Ctr West, Dublin 4, Ireland. [Walter, Stephanie; Kahla, Amal; Arunachalam, Chanemoughasoundharam; Perochon, Alexandre; Khan, Mojibur R.; Doohan, Fiona M.] Natl Univ Ireland Univ Coll Dublin, Sch Biol & Environm Sci, Sci Ctr West, Dublin 4, Ireland. [Scofield, Steven R.] ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Scofield, Steven R.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Doohan, FM (reprint author), Natl Univ Ireland Univ Coll Dublin, UCD Earth Inst, Sci Ctr West, Dublin 4, Ireland. EM fiona.doohan@ucd.ie RI Scofield, Steven/C-3868-2016 FU Science Foundation Ireland [10/IN.1/B3028] FX This work was funded by the Science Foundation Ireland project 10/IN.1/B3028. We thank Brian Fagan and Damian Egan, University College Dublin, Ireland, for technical assistance. We thank the Wheat Genetics Resource Center at Kansas State University (Manhattan, Kansas, USA) as well as Hermann Buerstmayr (IFA-Tulln, Austria) for providing wheat seeds. The authors declare they have no competing interests. NR 61 TC 9 Z9 10 U1 4 U2 28 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAY PY 2015 VL 66 IS 9 BP 2583 EP 2593 DI 10.1093/jxb/erv048 PG 11 WC Plant Sciences SC Plant Sciences GA CI4SB UT WOS:000354742200017 PM 25732534 ER PT J AU Lu, WH Diep, CS McKyer, ELJ AF Lu, Wenhua Diep, Cassandra S. McKyer, E. Lisako J. TI Risk Factors for Childhood Obesity among Asian Americans: A Systematic Review of Literature and Recommendations for Health Care Research SO JOURNAL OF HEALTH CARE FOR THE POOR AND UNDERSERVED LA English DT Review DE Asian Americans; childhood obesity; risk factors; systematic review ID BODY-MASS INDEX; FEEDING PRACTICES; CHINESE-AMERICAN; SOCIOECONOMIC-STATUS; ADOLESCENT OBESITY; PHYSICAL-ACTIVITY; UNITED-STATES; US CHILDREN; OVERWEIGHT; ACCULTURATION AB Childhood obesity has become an epidemic across all racial/ethnic groups in the U.S., including Asian Americans. With different cultures, beliefs, and lifestyles, Asian Americans may face unique sets of risk factors for childhood obesity This review critically assesses and summarizes the literature on risk factors for childhood obesity among Asian Americans. Among the 14 studies identified, five (35.7%) used national or state-level data, seven (50%) targeted Chinese Americans, and two (14.3%) focused on Hmong Americans. Multiple risk factors for childhood obesity among Asian Americans were reported, including acculturation, generational status, and family functioning. Limitations of the literature included use of small samples, scarcity of ethnically specific data for Asian American subgroups, shortage of qualitative studies, and lack of theoretical foundation. More disaggregated studies are needed to examine the important variability that may exist in risk factors for childhood obesity among Asian American subgroups. C1 [Lu, Wenhua] NYU, Silver Sch Social Work, New York, NY 10003 USA. [Diep, Cassandra S.] Baylor Coll Med, Dept Family & Community Med, Houston, TX 77030 USA. [Diep, Cassandra S.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [McKyer, E. Lisako J.] Texas A&M Univ, Dept Hlth & Kinesiol, Transdisciplinary Ctr Hlth Equ Res, College Stn, TX 77843 USA. RP Lu, WH (reprint author), NYU, Silver Sch Social Work, New York, NY 10003 USA. EM w.lu@nyu.edu FU National Research Service Award [T32 HP10031] FX Cassandra S. Diep was supported fully by a Primary Care Research Training Grant from National Research Service Award (#T32 HP10031). NR 52 TC 0 Z9 0 U1 2 U2 9 PU JOHNS HOPKINS UNIV PRESS PI BALTIMORE PA JOURNALS PUBLISHING DIVISION, 2715 NORTH CHARLES ST, BALTIMORE, MD 21218-4363 USA SN 1049-2089 EI 1548-6869 J9 J HEALTH CARE POOR U JI J. Health Care Poor Underserved PD MAY PY 2015 VL 26 IS 2 SU S BP 171 EP 190 PG 20 WC Health Policy & Services; Public, Environmental & Occupational Health SC Health Care Sciences & Services; Public, Environmental & Occupational Health GA CI2OR UT WOS:000354587400015 PM 25981097 ER PT J AU Mascarin, GM Jackson, MA Kobori, NN Behle, RW Delalibera, I AF Mascarin, Gabriel Moura Jackson, Mark Alan Kobori, Nilce Naomi Behle, Robert Warren Delalibera Junior, Italo TI Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Mycopesticides; Dimorphic growth; Submerged fermentation; Desiccation tolerance; Silverleaf whitefly; Storage stability ID FUNGUS PAECILOMYCES-FUMOSOROSEUS; ENTOMOPATHOGENIC FUNGI; BEMISIA-ARGENTIFOLII; BIOLOGICAL-CONTROL; METARHIZIUM-ANISOPLIAE; SUBMERGED CULTURE; GROWTH; MEDIA; VIRULENCE; CONIDIA AB A major constraint to the commercial use of fungal biocontrol agents is the availability of low-cost production media and processes. Previous attempts in producing Beauveria blastospores using liquid culture fermentation processes required long fermentation times (6-8 days) and produced cells that had poor survival after desiccation and storage. In this study, isolates of Beauveria bassiana and Isaria fumosorosea were evaluated for blastospore yield, desiccation tolerance, storage stability, and biocontrol efficacy using fermentation media containing acid hydrolyzed casein or cottonseed flour as the nitrogen source. Cultures of B. bassiana and I. fumosorosea grown in media containing cottonseed flour produced high blastospore concentrations (>1 x 10(9) mL(-1)) after 3 days which is comparatively less expensive nitrogen source than acid hydrolyzed casein. The resultant air-dried blastospores (<3% moisture) of all fungal isolates survived drying (61-86% viability), irrespective of the nitrogen source tested. Storage stability at 4 degrees C varied with nitrogen source and fungal strain. Air-dried blastospores of B. bassiana strains showed half-lives >14 months in contrast to 9.2-13.1 months for I. fumosorosea. Blastospores of B. bassiana and I. fumosorosea killed Bemisia tabaci whitefly nymphs faster and required lower concentrations compared with aerial conidia. Our findings support the use of liquid culture fermentation as a cost-effective process to rapidly produce high yields of stable and infective blastospores of either B. bassiana or I. fumosorosea. These results support further evaluation of blastospore sprayable formulations for the control of soft-bodied insects. (C) 2014 Elsevier Inc. All rights reserved. C1 [Mascarin, Gabriel Moura] Embrapa Rice & Beans, BR-75375000 Santo Antonio De Goias, GO, Brazil. [Mascarin, Gabriel Moura; Jackson, Mark Alan; Kobori, Nilce Naomi; Behle, Robert Warren] ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Mascarin, Gabriel Moura; Delalibera Junior, Italo] Univ Silo Paulo ESALQ USP, Escola Super Agr Luiz de Queiroz, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP, Brazil. RP Mascarin, GM (reprint author), Embrapa Rice & Beans, GO-462,Km 12,CP 179, BR-75375000 Santo Antonio De Goias, GO, Brazil. EM gabriel.mascarin@embrapa.br; mark.jackson@ars.usda.gov RI Mascarin, Gabriel/E-4646-2015 OI Mascarin, Gabriel/0000-0003-3517-6826 FU Empresa Brasileira de Pesquisa Agropecuaria (Embrapa, DF, Brazil) FX Empresa Brasileira de Pesquisa Agropecuaria (Embrapa, DF, Brazil) provided fellowship to the first author. The authors appreciate Dr. Steven P. Arthurs (University of Florida) for sending the whitefly founders to set up the colony. NR 35 TC 5 Z9 5 U1 4 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2015 VL 127 BP 11 EP 20 DI 10.1016/j.jip.2014.12.001 PG 10 WC Zoology SC Zoology GA CI1KQ UT WOS:000354503500003 PM 25497914 ER PT J AU Qiu, L Hou, LL Zhang, BY Liu, L Li, B Deng, P Ma, WH Wang, XP Fabrick, JA Chen, LZ Lei, CL AF Qiu, Lin Hou, Leilei Zhang, Boyao Liu, Lang Li, Bo Deng, Pan Ma, Weihua Wang, Xiaoping Fabrick, Jeffrey A. Chen, Lizhen Lei, Chaoliang TI Cadherin is involved in the action of Bacillus thuringiensis toxins Cry1Ac and Cry2Aa in the beet armyworm, Spodoptera exigua SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Bacillus thuringiensis; Cry toxin; Cadherin; Spodoptera exigua; Bt receptor; RNA interference ID BORDER MEMBRANE-VESICLES; HELICOVERPA-ARMIGERA LEPIDOPTERA; RECEPTOR-BINDING PROPERTIES; COTTONS EXPRESSING ONE; PINK-BOLLWORM; INSECT RESISTANCE; HELIOTHIS-VIRESCENS; CROSS-RESISTANCE; DELTA-ENDOTOXIN; BT COTTON AB Bacillus thuringiensis (Bt) insecticidal crystal (Cry) proteins are effective against some insect pests in sprays and transgenic crops, although the evolution of resistance could threaten the long-term efficacy of such Bt use. One strategy to delay resistance to Bt crops is to "pyramid" two or more Bt proteins that bind to distinct receptor proteins within the insect midgut. The most common Bt pyramid in cotton (Gossypium hirsutum L) employs Cry1 Ac with Cry2Ab to target several key lepidopteran pests, including the beet armyworm, Spodoptera exigua (Hubner), which is a serious migratory pest of many vegetable crops and is increasingly important in cotton in China. While cadherin and aminopeptidase-N are key receptors of Cry1 toxins in many lepidopterans including S. exigua, the receptor for Cry2A toxins remains poorly characterized. Here, we show that a heterologous expressed peptide corresponding to cadherin repeat 7 to the membrane proximal extracellular domain (CR7-MPED) in the S. exigua cadherin 1b (SeCad1b) binds Cry1Ac and Cry2Aa. Moreover, SeCad1b transcription was suppressed in S. exigua larvae by oral RNA interference and susceptibility to Cry1 Ac and Cry2Aa was significantly reduced. These results indicate that SeCad1b plays important functional roles of both Cry1Ac and Cry2Aa, having major implications for resistance management for S. exigua in Bt crops. (C) 2015 Elsevier Inc. All rights reserved. C1 [Qiu, Lin; Hou, Leilei; Zhang, Boyao; Liu, Lang; Li, Bo; Deng, Pan; Ma, Weihua; Wang, Xiaoping; Chen, Lizhen; Lei, Chaoliang] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Fabrick, Jeffrey A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Fabrick, JA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM jeff.fabrick@ars.usda.gov; lzchen@mail.hzau.edu.cn FU National Natural Science Foundation of China [31101445] FX We thank Prof. Guoqing Li from Nanjing Agriculture University (Nanjing, China) for providing the dsRNA expression vector. We thank Dale Spurgeon for comments on an earlier version of the manuscript. Financial support was provided by the National Natural Science Foundation of China (grant no. 31101445). This is a cooperative investigation between Huazhong Agricultural University and the USDA-ARS. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 85 TC 6 Z9 7 U1 4 U2 36 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2015 VL 127 BP 47 EP 53 DI 10.1016/j.jip.2015.02.009 PG 7 WC Zoology SC Zoology GA CI1KQ UT WOS:000354503500008 PM 25754522 ER PT J AU Guo, J Wu, J Chen, YP Evans, JD Dai, RG Luo, WH Li, JL AF Guo, Jun Wu, Jie Chen, Yanping Evans, Jay D. Dai, Rongguo Luo, Wenhua Li, Jilian TI Characterization of gut bacteria at different developmental stages of Asian honey bees, Apis cerana SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Apis cerana; Dynamics; Gut microbiota ID DIVISION-OF-LABOR; PAENIBACILLUS-LARVAE; MICROBIAL SYMBIONTS; AMERICAN FOULBROOD; AGE POLYETHISM; BUMBLE BEES; MELLIFERA L; DIVERSITY; COLONIES; COMMUNITIES AB Previous surveys have shown that adult workers of the Asian honey bee Apis cerana harbor four major gut microbes (Bifidobacterium, Snodgrassella alvi, Gilliamella apicola, and Lactobacillus). Using quantitative PCR we characterized gut bacterial communities across the life cycle of A. cerana from larvae to workers. Our results indicate that the presence and quantity of these four bacteria were low on day 1, increased rapidly after day 5, and then peaked during days 10-20. They stabilized from days 20-25 or days 25-30, then dropped to a low level at day 30. In addition, the larvae infected by Sacbrood virus or European foulbrood had significantly lower copies of 16S rRNA genes than healthy individuals. (C) 2015 Elsevier Inc. All rights reserved. C1 [Guo, Jun; Wu, Jie; Li, Jilian] Chinese Acad Agr Sci, Key Lab Pollinating Insect Biol, Minist Agr, Inst Apicultural Res, Beijing 100093, Peoples R China. [Guo, Jun; Dai, Rongguo; Luo, Wenhua] Chongqing Acad Anim Sci, Inst Econ Anim, Chongqing 402460, Peoples R China. [Chen, Yanping; Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. RP Wu, J (reprint author), Chinese Acad Agr Sci, Key Lab Pollinating Insect Biol, Minist Agr, Inst Apicultural Res, Beijing 100093, Peoples R China. EM apis@vip.sina.com; bumblebeeljl@hotmail.com RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU Agricultural Science and Technology Innovation Program [CAAS-ASTIP-2015-IAR]; Chinese "948" Ministry of Agriculture Project [125161029]; China Agriculture Research System [CARS-45] FX We would like to thank Tang Hong, Ren Qin and Cao Lan for collecting honeybee samples. We thank Nancy A. Moran and J. Elijah Powell for very useful comments on the manuscript. This study was funded by Agricultural Science and Technology Innovation Program (No. CAAS-ASTIP-2015-IAR), Chinese "948" Ministry of Agriculture Project (No. 125161029) and China Agriculture Research System (CARS-45). NR 37 TC 4 Z9 4 U1 11 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2015 VL 127 BP 110 EP 114 DI 10.1016/j.jip.2015.03.010 PG 5 WC Zoology SC Zoology GA CI1KQ UT WOS:000354503500017 PM 25805518 ER PT J AU Kao, CC Hsu, JW Dwarkanath, P Karnes, JM Baker, TM Bohren, KM Badaloo, A Thame, MM Kurpad, AV Jahoor, F AF Kao, Christina C. Hsu, Jean W. Dwarkanath, Pratibha Karnes, Jeffrey M. Baker, Tameka M. Bohren, Kurt M. Badaloo, Asha Thame, Minerva M. Kurpad, Anura V. Jahoor, Farook TI Indian Women of Childbearing Age Do Not Metabolically Conserve Arginine as Do American and Jamaican Women SO JOURNAL OF NUTRITION LA English DT Article DE arginine; arginase; American; Jamaican; Indian women ID TANDEM MASS-SPECTROMETRY; LEAN BODY-MASS; NITRIC-OXIDE; HUMAN-PREGNANCY; ARGINASE; CREATINE; DEFICIENCY; CITRULLINE; KINETICS; PROTEIN AB Background: In a previous study in pregnant American women, we reported that arginine flux and nitric oxide synthesis increased in trimester 2. More recently, we reported that Indian women do not increase arginine flux during pregnancy as their American or Jamaican counterparts do. Objective: The purpose of this study was to determine whether Indian women of childbearing age are producing less arginine and/or catabolizing more arginine and therefore have less available for anabolic pathways than do Jamaican and American women. Methods: Thirty healthy women aged 28.3 +/- 0.8 y from the United States, India, and Jamaica (n = 10/group) were given 6 h primed, constant intravenous infusions of guanidino-N-15(2)-arginine, 5,5-H-2(2)-citrulline, N-15(2)-ornithine, and ring-H-2(5)-phenylalanine, in addition to primed, oral doses of U-C-13(6)-arginine in both the fasting and postprandial states. An oral dose of deuterium oxide was also given to determine fat-free mass (FFM). Results: Compared with American women, Indian and Jamaican women had greater ornithine fluxes (mu mol . kg fat FFM-1 . h(-1)) in the fasting and postprandial states (27.3 +/- 2.5 vs. 39.6 +/- 3.7 and 37.2 +/- 2.0, respectively, P = 0.01), indicating greater arginine catabolism. However, Jamaican women had a higher endogenous arginine flux than did Indian and American women in the fasting (66.1 +/- 3.1 vs. 54.2 +/- 3.1 and 56.1 +/- 2.1, respectively, P= 0.01) and postprandial (53.8 +/- 2.2 vs. 43.7 +/- 4.9 and 42.8 +/- 3.1, respectively, P = 0.06) states. As a consequence, Indian women had lower arginine bioavailability (mu mol . kg FFM-1 h(-1)) in the fasting state (42.0 +/- 2.6) than did American (49.9 +/- 1.3, P = 0.045) and Jamaican (55.5 +/- 3.5, P = 0.004) women, as well as in the postprandial state (40.7 +/- 3.5 vs. 51.8 +/- 1.2 and 57.5 +/- 3.2, respectively, P = 0.001)., Conclusion: Compared with American and Jamaican women, Indian women of childbearing age have a decreased arginine supply because of increased arginine catabolism without an increase in arginine flux. C1 [Kao, Christina C.] Baylor Coll Med, Dept Med, Sect Pulm Crit Care & Sleep Med, Houston, TX 77030 USA. [Kao, Christina C.; Hsu, Jean W.; Karnes, Jeffrey M.; Bohren, Kurt M.; Jahoor, Farook] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Dwarkanath, Pratibha; Kurpad, Anura V.] St Johns Natl Acad Hlth Sci, St Johns Res Inst, Bangalore, Karnataka, India. [Baker, Tameka M.; Thame, Minerva M.] Univ W Indies, Dept Child & Adolescent Hlth, Kingston 7, Jamaica. [Badaloo, Asha] Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. RP Jahoor, F (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM fjahoor@bcm.edu FU Bill and Melinda Gates Foundation; USDA Agricultural Research Service [58-6250-6001] FX This work was supported by the Bill and Melinda Gates Foundation as well as by federal funds from the USDA Agricultural Research Service under cooperative agreement number 58-6250-6001. NR 29 TC 2 Z9 2 U1 1 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2015 VL 145 IS 5 BP 884 EP 892 DI 10.3945/jn.114.208231 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH4OO UT WOS:000354013000007 PM 25833892 ER PT J AU Glahn, RP AF Glahn, Raymond P. TI Iron Biofortification and Revisiting the Accuracy of Extrinsic Labeling in Studies of Iron Absorption SO JOURNAL OF NUTRITION LA English DT Letter ID PHASEOLUS-VULGARIS; FOOD; BIOAVAILABILITY; FORTIFICATION; BEANS; WOMEN; TAG C1 [Glahn, Raymond P.] ARS, USDA, Ithaca, NY 14853 USA. [Glahn, Raymond P.] Robert Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Glahn, RP (reprint author), ARS, USDA, Ithaca, NY 14853 USA. EM rpg3@cornell.edu NR 8 TC 1 Z9 1 U1 1 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2015 VL 145 IS 5 BP 1025 EP 1026 DI 10.3945/jn.114.204529 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH4OO UT WOS:000354013000026 PM 25934664 ER PT J AU Abernathy, T AF Abernathy, Toni TI Responsibilities of the USDA-Food and Nutrition Service in Nutrition Assistance Response to Natural Disasters SO JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY LA English DT Article DE disaster; nutrition assistance; mass feeding AB USDA makes sure that nutritious USDA Foods are made available to States, Indian Tribal Organizations and Emergency Feeding Organizations to help feed survivors of natural disasters and other emergencies when needed. C1 USDA, Off Emergency Management, Food & Nutr Serv, Alexandria, VA 22302 USA. RP Abernathy, T (reprint author), USDA, Off Emergency Management, Food & Nutr Serv, 3101 Pk Ctr Dr, Alexandria, VA 22302 USA. EM Toni.Abernathy@fns.usda.gov NR 0 TC 0 Z9 0 U1 3 U2 5 PU CENTER ACADEMIC PUBL JAPAN PI TOKYO PA 2-4-16 YAYOI, BUNKYO-KU, TOKYO, 113-0032, JAPAN SN 0301-4800 EI 1881-7742 J9 J NUTR SCI VITAMINOL JI J. Nutr. Sci. Vitaminol. PD MAY PY 2015 VL 61 SU S BP S14 EP S14 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CI8KH UT WOS:000355020700007 PM 26598830 ER PT J AU Waterland, R AF Waterland, Robert TI Early Nutritional Influences on Human Developmental Epigenetics SO JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY LA English DT Article DE DNA methylation; epigenetic development C1 Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med,Dept Pediat & Mol & Human Genet, Houston, TX 77030 USA. RP Waterland, R (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med,Dept Pediat & Mol & Human Genet, Houston, TX 77030 USA. EM waterland@bcm.edu NR 0 TC 0 Z9 0 U1 0 U2 5 PU CENTER ACADEMIC PUBL JAPAN PI TOKYO PA 2-4-16 YAYOI, BUNKYO-KU, TOKYO, 113-0032, JAPAN SN 0301-4800 EI 1881-7742 J9 J NUTR SCI VITAMINOL JI J. Nutr. Sci. Vitaminol. PD MAY PY 2015 VL 61 SU S BP S82 EP S82 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CI8KH UT WOS:000355020700034 PM 26598899 ER PT J AU Williams, JD Robertson, DS Wuest, SB AF Williams, J. D. Robertson, D. S. Wuest, S. B. TI Biofuel feedstock production potential in stream buffers of the inland Pacific Northwest: Productivity and management issues with invasive plants SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE multifunctional agricultural systems; riparian buffer; stream buffer; tall wheatgrass; weed management ID SOIL ORGANIC-CARBON; THERMOCHEMICAL CONVERSION; UNITED-STATES; BIOMASS; RESTORATION; HABITAT; NITROGEN; TILLAGE AB Stream buffers increase the complexity of agroecosystems, providing wildlife habitat, sediment and nutrient sinks to improve water quality, and potentially lignocellulosic feedstock for biofuels. The establishment of stream buffers can be difficult, with weeds competing with desired species and increasing weed pressure in the production fields and contaminating feedstocks. Our objective was to evaluate the success of establishing two different feedstock alternatives, alfalfa (ALF; Medicago sativa L., cultivar Ladak) and tall wheatgrass (TWG; Agropyron elongatum Podp., cultivar Alkar), plus a mix of alfalfa and tall wheatgrass (MIX) at two sites within each of three precipitation zones in the Pacific Northwest (PNW). We evaluated productivity, stand establishment, and weed infestation using a split plot, randomized complete block design. Establishment of targeted species was most successful and productivity was highest in the intermediate precipitation zone. Lignocellulosic feedstock produced by TWG (7.52 +/- 2.07 Mg ha(-1) [3.35 +/- 0.92 tn ac(-1)]) and MIX (7.45 +/- 2.00 Mg ha(-1) [3.32 +/- 0.89 tn ac(-1)]) produced significantly more lignocellulosic feedstock than ALF (1.68 +/- 0.79 Mg ha(-1) [0.75 +/- 0.35 tn ac(-1)]) and SG (2.62 +/- 0.99 Mg ha(-1) [1.17 +/- 0.44 tn ac(-1)]). The best competition against invasive annual species was obtained in the TWG and MIX treatments, with tall wheatgrass providing the bulk of biomass in either treatment. Productivity was acceptable where naturally occurring subirrigation was present and contributed to crop germination and establishment in the low precipitation zone, but weed pressure was substantial and caused near to complete crop failure in the driest plots. A high level of weed management will be required to produce monocultures of lignocellulosic feedstocks in stream buffers. C1 [Williams, J. D.; Robertson, D. S.; Wuest, S. B.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. RP Williams, JD (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. FU USDA ARS national program Agricultural Competitiveness and Sustainability (NP) [216] FX We thank Dave Huggins, USDA Agricultural Research Service (ARS), Pullman, Washington, for providing research space at the USDA Conservation and WSU Cook Farm near Pullman, Washington, and Cunningham Sheep Farms, Newtson Farms, Bracher Farms, and Bill Lorenzen, farmer in Pendleton, Oregon, for providing research sites in Oregon. We thank Chris Hoag (wetlands specialist, USDA NRCS, Aberdeen, Idaho) and Mark Stannard (plant science specialist, USDA NRCS, Pullman, Washington) for their informative discussion and support for this research. We also thank students Seren Hansen and Stefka Waite, USDA ARS agricultural research technicians Bob Correa (Pendleton, Oregon) and Dave Uberuaga (Pullman, Washington) for field work, resource coordinator Heidi Hartman (Oregon Division of State Lands), and botanists Joan Frazee and Mark Darrach (USDA Forest Service, Pendleton, Oregon) for plant identification. This research was funded by USDA ARS national program Agricultural Competitiveness and Sustainability (NP#216). USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 48 TC 2 Z9 2 U1 3 U2 10 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2015 VL 70 IS 3 BP 156 EP 169 DI 10.2489/jswc.70.3.156 PG 14 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CH5TO UT WOS:000354098400006 ER PT J AU Strickland, TC Scully, BT Hubbard, RK Sullivan, DG Abdo, Z Savabi, MR Lee, RD Olson, DM Hawkins, GL AF Strickland, T. C. Scully, B. T. Hubbard, R. K. Sullivan, D. G. Abdo, Z. Savabi, M. R. Lee, R. D. Olson, D. M. Hawkins, G. L. TI Effect of conservation practices on soil carbon and nitrogen accretion and crop yield in a corn production system in the southeastern coastal plain, United States SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE fuzzy c-means clustering; Greenhouse gas Reduction through Agricultural Carbon Enhancement network (GRACEnet); Long-Term Agroecosystem Research (LTAR) Network; low input farming ID MICROBIAL BIOMASS NITROGEN; ELECTRICAL-CONDUCTIVITY; CHLOROFORM FUMIGATION; ORGANIC-CARBON; VARIABILITY; TILLAGE; MANAGEMENT; SEQUESTRATION; DELINEATION; POOLS AB Although conservation tillage is widely believed to be an agricultural management practice effective for increasing soil carbon (C) accretion and associated soil quality, there is limited research to determine whether conservation tillage increases net C accretion versus simply altering the distribution of C content by soil depth. We implemented conservation farming practices (winter cover cropping plus strip tillage) for a nonirrigated corn (Zea mays L.) production system in the southeastern coastal plain of Georgia, United States, that had been previously managed under a conventional plow and harrow tillage regime. Total soil C and nitrogen (N) were measured on samples collected from 0 to 65 cm (0 to 25.6 in) at 57 sites before and after five years under conservation farming practices. Crop yield, winter and summer aboveground crop biomass production, and biomass C and N content were also measured annually at each site. Soil C increased an average of 20 Mg ha(-1) (8.9 tn ac(-1); 6 to 62 Mg C ha(-1) [2.6 to 27.6 tn C ac(-1)], depending upon slope position) and was associated with a N increase of 2 Mg ha(-1) (0.89 tn ac(-1)). Although 72% to 80% of the C accretion was in the top 35 cm (13.8 in), 3 to 6 Mg C ha(-1) (1.3 to 2.6 tn C ac(-1)) was accreted from 35 to 65 cm (13.8 to 25.6 in). The soil C accreted during the study amounted to 36% of the net biomass C produced. Corn yield increased 2,200 kg ha(-1) (1,964 lb ac(-1)) depending upon slope position (1,200 to 2,500 kg ha(-1) [1,071 to 2,232 lb ac(-1)]) during the same time. Analysis indicated that soil C content from 15 to 35 cm (5.9 to 13.8 in) was the soil parameter primarily associated with corn yield. Season rainfall from planting to corn silking stage for both corn production years was the lowest in the past 45 years (20 to 25 cm [7.8 to 9.8 in] below the net crop demand) suggesting that soil C-mediated increase in plant-available soil water was a mechanism contributing to improved corn yield. Calculated estimates (from soil clay, sand, and C content) of increased soil water holding capacity suggest that C accretion in the top 35 cm (13.8 in) of soil potentially increased water storage enough to supply up to four days' worth of additional crop water demand. These results indicated that conservation farming practices can increase soil C and N accretion in degraded sandy soils of the humid southeastern United States coastal plain, and that increased soil C may potentially mitigate the deleterious effects of short-term rainfall deficits in nonirrigated production systems. C1 [Strickland, T. C.] USDA ARS, Southeast Watershed Res Lab, Tifton, GA 31793 USA. [Scully, B. T.; Hubbard, R. K.; Savabi, M. R.] USDA ARS, US Hort Res Lab, Ft Pierce, FL USA. [Sullivan, D. G.] TurfScout LLC, Greensboro, NC USA. [Abdo, Z.] USDA ARS, Southeast Area Statistician, Athens, GA 30613 USA. [Lee, R. D.; Hawkins, G. L.] Univ Georgia, Tifton, GA USA. [Olson, D. M.] USDA ARS, Crop Protect, Tifton, GA 31793 USA. [Olson, D. M.] USDA ARS, Management Res Unit, Tifton, GA 31793 USA. RP Strickland, TC (reprint author), USDA ARS, Southeast Watershed Res Lab, Tifton, GA 31793 USA. FU Georgia Agricultural Commodity Commissions for Corn [58-6602-8-109]; Georgia Agricultural Commodity Commissions for Peanut [58-6602-8-123] FX This research is a contribution of the USDA Agricultural Research Service GRACEnet Project and the USDA ARS Gulf Atlantic Coastal Plain Long-Term Agroecosystem Research site. Partial funding was provided by the Georgia Agricultural Commodity Commissions for Corn (agreement #58-6602-8-109) and Peanut (agreement #58-6602-8-123). The authors are grateful for the dedicated assistance of: Lorine Lewis, Coby Smith, Bobby Shiver, Ricky Fletcher, DeeAnn Webb (all research technicians with the USDA ARS Southeast Watershed Research Laboratory in Tifton, Georgia), Thoris Green, Andy Hornbuckle (research technicians with the USDA ARS Crop Protection and Management Research Unit in Tifton, Georgia), Bobby Hagler (research technician with the USDA ARS National Peanut Research Laboratory in Dawson, Georgia), Trevor Perla (research technician with the USDA ARS Crop Genetics and Breeding Research Unit in Tifton, Georgia), Gary Murphy (research technician with the University of Georgia Department of Crop and Soil Science), Nicole Jennings, Anthony O'Day, Janzen Brewer, Andrew Stinson, Chris Larson, Bryant Luke, Clay Lott, Brock Ward, Brian Sawyer, and Dakota Garrick (all student trainees with the USDA ARS Southeast Watershed Research Laboratory). The authors are also grateful to John Baker (supervisory soil physicist with the USDA ARS Soil and Water Management Research Unit in St. Paul, Minnesota) and Diane Rowland (professor in the Agronomy Department at the University of Florida in Gainesville, Florida) for presubmission review of this manuscript. NR 41 TC 1 Z9 2 U1 4 U2 33 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2015 VL 70 IS 3 BP 170 EP 181 DI 10.2489/jswc.70.3.170 PG 12 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CH5TO UT WOS:000354098400007 ER PT J AU Noutsos, C Perera, AM Nikolau, BJ Seaver, SMD Ware, DH AF Noutsos, Christos Perera, Ann M. Nikolau, Basil J. Seaver, Samuel M. D. Ware, Doreen H. TI Metabolomic Profiling of the Nectars of Aquilegia pubescens and A-Canadensis SO PLOS ONE LA English DT Article ID SUGAR COMPOSITION; AMINO-ACIDS; FLOWERS; HUMMINGBIRDS; POLLINATION; ANNOTATION; PARENTS; HYBRIDS; SEED AB To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator-plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the Aquilegia genus has been used as a key model for speciation studies. In this study, we defined the metabolomic profiles of flower samples of two Aquilegia species, A. Canadensis and Aquilegia pubescens. We identified a total of 75 metabolites that were classified into six main categories: organic acids, fatty acids, amino acids, esters, sugars, and unknowns. The mean abundances of 25 of these metabolites were significantly different between the two species, providing insights into interspecies variation in floral chemistry. Using the PlantSEED biochemistry database, we found that the majority of these metabolites are involved in biosynthetic pathways. Finally, we explored the annotated genome of A. coerulea, using the PlantSEED pipeline and reconstructed the metabolic network of Aquilegia. This network, which contains the metabolic pathways involved in generating the observed chemical variation, is now publicly available from the DOE Systems Biology Knowledge Base (KBase; http://kbase.us). C1 [Noutsos, Christos; Ware, Doreen H.] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Perera, Ann M.; Nikolau, Basil J.] Iowa State Univ, WM Keck Metabol Res Lab, Ames, IA USA. [Nikolau, Basil J.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA USA. [Seaver, Samuel M. D.] Argonne Natl Lab, Math & Comp Sci Div, Argonne, IL 60439 USA. [Seaver, Samuel M. D.] Univ Chicago, Computat Inst, Chicago, IL 60637 USA. [Ware, Doreen H.] USDA ARS, NAA, Robert W Holley Ctr, Ithaca, NY 14853 USA. RP Noutsos, C (reprint author), Cold Spring Harbor Lab, POB 100, Cold Spring Harbor, NY 11724 USA. EM cnoutsos@cshl.edu FU National Science Foundation of United States of America under Division of Biological Infrastructure [DBI-1265382]; Department of Energy [DE-AC02-98CH10886]; NSF [BDI-1265383]; iPlant Collaborative Project FX This work was funded by grants from the National Science Foundation of United States of America under the Division of Biological Infrastructure to Dr. Doreen Ware who is Co-Principal Investigator to the DBI-1265382. Financial support was provided by the Department of Energy (DE-AC02-98CH10886) for Seaver SMD and to Christos Noutsos from the NSF by the BDI-1265383, the iPlant Collaborative Project. Basil J. Nikolau and and Ann M. Perera received no specific funding for this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 3 Z9 3 U1 3 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 1 PY 2015 VL 10 IS 5 AR e0124501 DI 10.1371/journal.pone.0124501 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH2XA UT WOS:000353887100046 PM 25933103 ER PT J AU Kappes, MA Faaberg, KS AF Kappes, Matthew A. Faaberg, Kay S. TI PRRSV structure, replication and recombination: Origin of phenotype and genotype diversity SO VIROLOGY LA English DT Review DE Porcine reproductive and respiratory syndrome virus; Arterivirus; Replication; Recombination; Heteroclite; Subgenomic RNA ID RESPIRATORY-SYNDROME-VIRUS; MESSENGER-RNA SYNTHESIS; EQUINE ARTERITIS VIRUS; TRANSCRIPTION-REGULATING SEQUENCES; INFECTIOUS-BRONCHITIS VIRUS; HETEROCLITE SUBGENOMIC RNAS; PORCINE EPIDEMIC ABORTION; BODY JUNCTION SEQUENCE; MYSTERY SWINE DISEASE; ISOLATE ATCC VR-2332 AB Porcine reproductive and respiratory disease virus (PRRSV) has the intrinsic ability to adapt and evolve. After 25 years of study, this persistent pathogen has continued to frustrate efforts to eliminate infection of herds through vaccination or other elimination strategies. The purpose of this review is to summarize the research on the virion structure, replication and recombination properties of PRRSV that have led to the extraordinary phenotype and genotype diversity that exists worldwide. Published by Elsevier Inc. C1 [Kappes, Matthew A.; Faaberg, Kay S.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Faaberg, KS (reprint author), USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM kay.faaberg@ars.usda.gov FU USDA Agricultural Research Service [3625-32000-108-00D]; BI Vetmedica, Inc. [991-3625-791]; National Pork Board [13-196] FX This work builds upon the doctoral thesis of Matthew Kappes, Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA, 2014, entitled "Identification and characterization of a novel structural protein of porcine reproductive and respiratory syndrome virus, the replicase nonstructural protein 2". Project 3625-32000-108-00D of the USDA Agricultural Research Service provided support for Dr. Faaberg. Dr. Kappes was supported by BI Vetmedica, Inc., (Grant no. 991-3625-791), The National Pork Board (Grant no. 13-196) and by the USDA Agricultural Research Service. USDA is an equal opportunity provider and employer. NR 182 TC 16 Z9 19 U1 8 U2 33 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2015 VL 479 SI SI BP 475 EP 486 DI 10.1016/j.virol.2015.02.012 PG 12 WC Virology SC Virology GA CI6YG UT WOS:000354909500042 PM 25759097 ER PT J AU Ramstein, GP Lipka, AE Lu, F Costich, DE Cherney, JH Buckler, ES Casler, MD AF Ramstein, Guillaume P. Lipka, Alexander E. Lu, Fei Costich, Denise E. Cherney, Jerome H. Buckler, Edward S. Casler, Michael D. TI Genome-Wide Association Study Based on Multiple Imputation with Low-Depth Sequencing Data: Application to Biofuel Traits in Reed Canarygrass SO G3-GENES GENOMES GENETICS LA English DT Article DE genome-wide association study; multiple imputation; genotyping by sequencing; bioenergy; Phalaris spp. ID FULLY CONDITIONAL SPECIFICATION; MISSING DATA; MULTIVARIATE IMPUTATION; CHEMICAL-COMPOSITION; POPULATION-STRUCTURE; GENETIC-VARIABILITY; BIOMASS YIELD; SWITCHGRASS; SELECTION; MODEL AB Genotyping by sequencing allows for large-scale genetic analyses in plant species with no reference genome, but sets the challenge of sound inference in presence of uncertain genotypes. We report an imputation-based genome-wide association study (GWAS) in reed canarygrass (Phalaris arundinacea L., Phalaris caesia Nees), a cool-season grass species with potential as a biofuel crop. Our study involved two linkage populations and an association panel of 590 reed canarygrass genotypes. Plants were assayed for up to 5228 single nucleotide polymorphism markers and 35 traits. The genotypic markers were derived from low-depth sequencing with 78% missing data on average. To soundly infer marker-trait associations, multiple imputation (MI) was used: several imputes of the marker data were generated to reflect imputation uncertainty and association tests were performed on marker effects across imputes. A total of nine significant markers were identified, three of which showed significant homology with the Brachypodium dystachion genome. Because no physical map of the reed canarygrass genome was available, imputation was conducted using classification trees. In general, MI showed good consistency with the complete-case analysis and adequate control over imputation uncertainty. A gain in significance of marker effects was achieved through MI, but only for rare cases when missing data were <45%. In addition to providing insight into the genetic basis of important traits in reed canarygrass, this study presents one of the first applications of MI to genome-wide analyses and provides useful guidelines for conducting GWAS based on genotyping-by-sequencing data. C1 [Ramstein, Guillaume P.; Casler, Michael D.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Lipka, Alexander E.; Lu, Fei; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Cherney, Jerome H.] Cornell Univ, Sch Integrat Plant Sci, Soil & Crops Sect, Ithaca, NY 14853 USA. [Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Costich, Denise E.] Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco 56237, Mexico. [Buckler, Edward S.] ARS, USDA, Ithaca, NY 14853 USA. [Casler, Michael D.] ARS, USDA, Madison, WI 53706 USA. RP Ramstein, GP (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM ramstein@wisc.edu OI Buckler, Edward/0000-0002-3100-371X FU US Department of Energy & Department of Agriculture Plant Feedstock Genomics for Bioenergy Program [DE-A-102-07ER64454]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture (Cen-USA) [2011-68005-30411]; USDA-ARS Congressionally allocated funds; Gabelman-Shippo Wisconsin Distinguished Graduate Fellowship at the University of Wisconsin-Madison FX The authors thank two anonymous reviewers for remarks and suggestions that greatly helped with improving the manuscript. This research was supported by the US Department of Energy & Department of Agriculture Plant Feedstock Genomics for Bioenergy Program Project Number DE-A-102-07ER64454, by Agriculture and Food Research Initiative Competitive Grant No. 2011-68005-30411 from the USDA National Institute of Food and Agriculture (Cen-USA), and by USDA-ARS Congressionally allocated funds. Mention of commercial products and organizations in this manuscript is solely to provide specific information. The USDA is an equal opportunity provider and employer. G.P.R. was supported by the Gabelman-Shippo Wisconsin Distinguished Graduate Fellowship at the University of Wisconsin-Madison. NR 80 TC 5 Z9 5 U1 1 U2 22 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD MAY 1 PY 2015 VL 5 IS 5 BP 891 EP 909 DI 10.1534/g3.115.017533 PG 19 WC Genetics & Heredity SC Genetics & Heredity GA CH8BX UT WOS:000354262000017 PM 25770100 ER PT J AU Kyung, KH Pradas, EM Kim, SG Lee, YJ Kim, KH Choi, JJ Cho, JH Chung, CH Barrangou, R Breidt, F AF Kyung, Kyu Hang Medina Pradas, Eduardo Kim, Song Gun Lee, Yong Jae Kim, Kyong Ho Choi, Jin Joo Cho, Joo Hyong Chung, Chang Ho Barrangou, Rodolphe Breidt, Frederick TI Microbial Ecology of Watery Kimchi SO JOURNAL OF FOOD SCIENCE LA English DT Article DE high-throughput sequencing; microbial ecology; watery kimchi ID FERMENTATION; BACTERIAL; DONGCHIMI AB The biochemistry and microbial ecology of 2 similar types of watery (mul) kimchi, containing sliced and unsliced radish and vegetables (nabak and dongchimi, respectively), were investigated. Samples from kimchi were fermented at 4, 10, and 20 degrees C were analyzed by plating on differential and selective media, high-performance liquid chromatography, and high-throughput DNA sequencing of 16S rDNA. Nabak kimchi showed similar trends as dongchimi, with increasing lactic and acetic acids and decreasing pH for each temperature, but differences in microbiota were apparent. Interestingly, bacteria from the Proteobacterium phylum, including Enterobacteriaceae, decreased more rapidly during fermentation at 4 degrees C in nabak cabbage fermentations compared with dongchimi. Although changes for Proteobacterium and Enterobacteriaceae populations were similar during fermentation at 10 and 20 degrees C, the homolactic stage of fermentation did not develop for the 4 and 10 degrees C samples of both nabak and dongchimi during the experiment. These data show the differences in biochemistry and microbial ecology that can result from preparation method and fermentation conditions of the kimchi, which may impact safety (Enterobacteriaceae populations may include pathogenic bacteria) and quality (homolactic fermentation can be undesirable, if too much acid is produced) of the product. In addition, the data also illustrate the need for improved methods for identifying and differentiating closely related lactic acid bacteria species using high-throughput sequencing methods. C1 [Kyung, Kyu Hang] Sejong Univ, Dept Food Sci, Seoul 143747, South Korea. [Medina Pradas, Eduardo] Inst Grasa CSIC, Seville 41012, Spain. [Kim, Song Gun; Lee, Yong Jae] Korea Res Inst Biosci & Biotechnol, Microbial Resource Ctr KCTC, Taejon 305806, South Korea. [Kim, Kyong Ho] Pukyong Natl Univ, Dept Microbiol, Busan 608737, South Korea. [Choi, Jin Joo; Cho, Joo Hyong; Chung, Chang Ho] Sejong Univ, Dept Culinary Sci & Food Serv Management, Seoul 143747, South Korea. [Medina Pradas, Eduardo; Barrangou, Rodolphe] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. [Breidt, Frederick] N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, Raleigh, NC 27695 USA. RP Breidt, F (reprint author), N Carolina State Univ, USDA ARS, SAA Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM Fred.Breidt@ars.usda.gov RI Barrangou, Rodolphe/I-2878-2014; Eduardo, Medina/D-3296-2014 OI Barrangou, Rodolphe/0000-0002-0648-3504; Eduardo, Medina/0000-0002-9978-4524 FU Agency for Korea Natl. Food Cluster; Pickle Packers Intl. Inc., Washington, D.C., U.S.A; Spanish Government (MECD) FX This work was carried out as part of the international collaborative R&D program funded by the Agency for Korea Natl. Food Cluster (2013), and supported in part by a grant from Pickle Packers Intl. Inc., Washington, D.C., U.S.A. The authors thank the Spanish Government (MECD) for the postdoctoral fellowship support for Dr. E. Medina-Pradas. NR 18 TC 2 Z9 2 U1 1 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2015 VL 80 IS 5 BP M1031 EP M1038 DI 10.1111/1750-3841.12848 PG 8 WC Food Science & Technology SC Food Science & Technology GA CH8PG UT WOS:000354297800015 PM 25847522 ER PT J AU Panagopoulos, Y Gassman, PW Jha, MK Kling, CL Campbell, T Srinivasan, R White, M Arnold, JG AF Panagopoulos, Yiannis Gassman, Philip W. Jha, Manoj K. Kling, Catherine L. Campbell, Todd Srinivasan, Raghavan White, Michael Arnold, Jeffrey G. TI A refined regional modeling approach for the Corn Belt - Experiences and recommendations for large-scale integrated modeling SO JOURNAL OF HYDROLOGY LA English DT Article DE Corn Belt; Large-scale calibration; Nonpoint source pollution; Refined modeling; SWAT ID MISSISSIPPI RIVER-BASIN; GULF-OF-MEXICO; WATER ASSESSMENT-TOOL; CONSERVATION PRACTICES; SENSITIVITY-ANALYSIS; SOURCE AREAS; SWAT; QUALITY; CALIBRATION; NUTRIENT AB Nonpoint source pollution from agriculture is the main source of nitrogen and phosphorus in the stream systems of the Corn Belt region in the Midwestern US. This region is comprised of two large river basins, the intensely row-cropped Upper Mississippi River Basin (UMRB) and Ohio-Tennessee River Basin (OTRB), which are considered the key contributing areas for the Northern Gulf of Mexico hypoxic zone according to the US Environmental Protection Agency. Thus, in this area it is of utmost importance to ensure that intensive agriculture for food, feed and biofuel production can coexist with a healthy water environment. To address these objectives within a river basin management context, an integrated modeling system has been constructed with the hydrologic Soil and Water Assessment Tool (SWAT) model, capable of estimating river basin responses to alternative cropping and/or management strategies. To improve modeling performance compared to previous studies and provide a spatially detailed basis for scenario development, this SWAT Corn Belt application incorporates a greatly refined subwatershed structure based on 12-digit hydrologic units or 'subwatersheds' as defined by the US Geological Service. The model setup, calibration and validation are time-demanding and challenging tasks for these large systems, given the scale intensive data requirements, and the need to ensure the reliability of flow and pollutant load predictions at multiple locations. Thus, the objectives of this study are both to comprehensively describe this large-scale modeling approach, providing estimates of pollution and crop production in the region as well as to present strengths and weaknesses of integrated modeling at such a large scale along with how it can be improved on the basis of the current modeling structure and results. The predictions were based on a semi-automatic hydrologic calibration approach for large-scale and spatially detailed modeling studies, with the use of the Sequential Uncertainty Fitting algorithm (SUFI-2) and the SWAT-CUP interface, followed by a manual water quality calibration on a monthly basis. The refined modeling approach developed in this study led to successful predictions across most parts of the Corn Belt region and can be used for testing pollution mitigation measures and agricultural economic scenarios, providing useful information to policy makers and recommendations on similar efforts at the regional scale. (C) 2015 Elsevier B.V. All rights reserved. C1 [Panagopoulos, Yiannis; Gassman, Philip W.; Kling, Catherine L.; Campbell, Todd] Iowa State Univ, Ctr Agr & Rural Dev, Ames, IA 50011 USA. [Jha, Manoj K.] North Carolina A&T State Univ, Dept Civil Engn, Greensboro, NC 27410 USA. [Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, College Stn, TX 77845 USA. [White, Michael; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Panagopoulos, Y (reprint author), Iowa State Univ, Ctr Agr & Rural Dev, Ames, IA 50011 USA. EM ypanag@iastate.edu RI Srinivasan, R/D-3937-2009; OI Kling, Catherine L/0000-0002-4785-7154 FU National Science Foundation [DEB1010259]; US Department of Agriculture, National Institute of Food and Agriculture [20116800230190]; US Environmental Protection Agency's Science to Achieve Results (STAR) [EPA G 1469 1 2008 35615 04666] FX This research was partially funded by the National Science Foundation, Award No. DEB1010259, Understanding Land Use Decisions & Watershed Scale Interactions: Water Quality in the Mississippi River Basin & Hypoxic Conditions in the Gulf of Mexico, by the US Department of Agriculture, National Institute of Food and Agriculture, Award No. 20116800230190, Climate Change, Mitigation, and Adaptation In Corn-Based Cropping Systems, and by the US Environmental Protection Agency's Science to Achieve Results (STAR) award (EPA G 1469 1 2008 35615 04666). USDA is an equal opportunity provider and employer. NR 67 TC 5 Z9 5 U1 6 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD MAY PY 2015 VL 524 BP 348 EP 366 DI 10.1016/j.jhydrol.2015.02.039 PG 19 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CI1KO UT WOS:000354503300027 ER PT J AU Cooperband, MF Hartness, A Zilkowski, B Lelito, J Cosse, A AF Cooperband, Miriam F. Hartness, Ashley Zilkowski, B. Lelito, Jon Cosse, Allard TI Attraction of Spathius Agrili Yang (Hymenoptera: Braconidae) To Male-Produced "Aggregation-Sex Pheromone": Differences Between the Sexes and Mating Status (vol 28, pg 167, 2015) SO JOURNAL OF INSECT BEHAVIOR LA English DT Correction C1 [Cooperband, Miriam F.; Hartness, Ashley] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Zilkowski, B.; Cosse, Allard] USDA ARS NCAUR, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. [Lelito, Jon] USDA APHIS PPQ Emerald Ash Borer Program, Brighton, MI 48116 USA. RP Cooperband, MF (reprint author), USDA APHIS PPQ CPHST, Otis Lab, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM miriam.f.cooperband@aphis.usda.gov NR 1 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD MAY PY 2015 VL 28 IS 3 BP 358 EP 358 DI 10.1007/s10905-015-9497-1 PG 1 WC Entomology SC Entomology GA CI3FW UT WOS:000354634100013 ER PT J AU Raiten, DJ Ashour, FAS Ross, AC Meydani, SN Dawson, HD Stephensen, CB Brabin, BJ Suchdev, PS van Ommen, B AF Raiten, Daniel J. Ashour, Fayrouz A. Sakr Ross, A. Catharine Meydani, Simin N. Dawson, Harry D. Stephensen, Charles B. Brabin, Bernard J. Suchdev, Parminder S. van Ommen, Ben CA INSPIRE Consultative Grp TI Inflammation and Nutritional Science for Programs/Policies and Interpretation of Research Evidence (INSPIRE) SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT Inflammation and Nutritional Science for Programs/Policies and Interpretation of Research Evidence (INSPIRE) Meeting CY NOV 28-30, 2012 CL Natl Inst Hlth, Bethesda, MD HO Natl Inst Hlth DE nutrition biomarkers; inflammation and nutrition; immune function and nutrients; BOND and inflammation/infection; assessment of micronutrient biomarkers ID C-REACTIVE PROTEIN; VITAMIN-A SUPPLEMENTATION; ACUTE-PHASE RESPONSE; TUMOR-NECROSIS-FACTOR; OBSTRUCTIVE PULMONARY-DISEASE; PLACEBO-CONTROLLED TRIAL; HUMAN-IMMUNODEFICIENCY-VIRUS; REGULATORY T-CELLS; NF-KAPPA-B; RANDOMIZED CONTROLLED-TRIAL AB An increasing recognition has emerged of the complexities of the global health agenda specifically, the collision of infections and noncommunicable diseases and the dual burden of over- and undernutrition. Of particular practical concern are both 1) the need for a better understanding of the bidirectional relations between nutritional status and the development and function of the immune and inflammatory response and 2) the specific impact of the inflammatory response on the selection, use, and interpretation of nutrient biomarkers. The goal of the Inflammation and Nutritional Science for Programs/Policies and Interpretation of Research Evidence (INSPIRE) is to provide guidance for those users represented by the global food and nutrition enterprise. These include researchers (bench and clinical), clinicians providing care/treatment, those developing and evaluating programs/interventions at scale, and those responsible for generating evidence-based policy. The INSPIRE process included convening 5 thematic working groups (WGs) charged with developing summary reports around the following issues: 1) basic overview of the interactions between nutrition, immune function, and the inflammatory response; 2) examination of the evidence regarding the impact of nutrition on immune function and inflammation; a evaluation of the impact of inflammation and clinical conditions (acute and chronic) on nutrition; 4) examination of existing and potential new approaches to account for the impact of inflammation on biomarker interpretation and use, and 5) the presentation of new approaches to the study of these relations. Each WG was tasked with synthesizing a summary of the evidence for each of these topics and delineating the remaining gaps in our knowledge. This review consists of a summary of the INSPIRE workshop and the WG deliberations. C1 [Raiten, Daniel J.; Ashour, Fayrouz A. Sakr] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD 20892 USA. [Ross, A. Catharine] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. [Ross, A. Catharine] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Ross, A. Catharine] Penn State Univ, Ctr Mol Immunol & Infect Dis, University Pk, PA 16802 USA. [Meydani, Simin N.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Dawson, Harry D.] USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Stephensen, Charles B.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Brabin, Bernard J.] Univ Liverpool, Liverpool Sch Trop Med, Child & Reprod Hlth Grp, Liverpool L3 5QA, Merseyside, England. [Brabin, Bernard J.] Univ Amsterdam, Acad Med Ctr, Global Child Hlth Grp, NL-1105 AZ Amsterdam, Netherlands. [Suchdev, Parminder S.] Emory Univ, Dept Pediat & Global Hlth, Atlanta, GA 30322 USA. [van Ommen, Ben] TNO, NL-3700 AJ Zeist, Netherlands. RP Raiten, DJ (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD 20892 USA. EM raitend@mail.nih.gov RI Calder, Philip/E-9739-2013; Rink, Lothar/C-1055-2014; Friis, Henrik/N-2047-2014; OI Calder, Philip/0000-0002-6038-710X; Friis, Henrik/0000-0002-2848-2940; Suchdev, Parmi/0000-0002-0350-3469 FU NIAID NIH HHS [R56 AI114972]; NICHD NIH HHS [R01 HD066982]; NIDDK NIH HHS [R56 DK041479] NR 798 TC 29 Z9 29 U1 3 U2 27 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2015 VL 145 IS 5 SU S BP 1039S EP 1108S DI 10.3945/jn.114.194571 PG 70 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH4OP UT WOS:000354013100001 PM 25833893 ER PT J AU Claycombe, KJ Brissette, CA Ghribi, O AF Claycombe, Kate J. Brissette, Catherine A. Ghribi, Othman TI Epigenetics of Inflammation, Maternal Infection, and Nutrition SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT Inflammation and Nutritional Science for Programs/Policies and Interpretation of Research Evidence (INSPIRE) Meeting CY NOV 28-30, 2012 CL Natl Inst Hlth, Bethesda, MD HO Natl Inst Hlth DE epigenetics; immunity; inflammation; nutrients; macrophages; obesity ID MESSENGER-RNA DECAY; IFN-GAMMA GENE; DETERMINES METHYLATION; HISTONE ACETYLATION; CYTOKINE PRODUCTION; INSULIN-RESISTANCE; HDAC INHIBITION; T-CELLS; EXPRESSION; RATS AB Studies have demonstrated that epigenetic changes such as DNA methylation, histone modification, and chromatin remodeling are linked to an increased inflammatory response as well as increased risk of chronic disease development. A few studies have begun to investigate whether dietary nutrients play a beneficial role by modifying or reversing epigenetically induced inflammation. Results of these studies show that nutrients modify epigenetic pathways. However, little is known about how nutrients modulate inflammation by regulating immune cell function and/or immune cell differentiation via epigenetic pathways. This overview will provide information about the current understanding of the role of nutrients in the epigenetic control mechanisms of immune function. C1 [Claycombe, Kate J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Brissette, Catherine A.; Ghribi, Othman] Univ N Dakota, Sch Med & Hlth Sci, Dept Basic Sci, Grand Forks, ND 58201 USA. RP Claycombe, KJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM kate.claycombe@ars.usda.gov FU USDA/Agricultural Research Service project plan [5450-51000-047-00D] FX Funding provided by USDA/Agricultural Research Service project plan 5450-51000-047-00D. NR 88 TC 4 Z9 4 U1 3 U2 17 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2015 VL 145 IS 5 SU S BP 1109S EP 1115S DI 10.3945/jn.114.194639 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH4OP UT WOS:000354013100002 PM 25833887 ER PT J AU Duffy, LC Raiten, DJ Hubbard, VS Starke-Reed, P AF Duffy, Linda C. Raiten, Daniel J. Hubbard, Van S. Starke-Reed, Pamela TI Progress and Challenges in Developing Metabolic Footprints from Diet in Human Gut Microbial Cometabolism SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT Inflammation and Nutritional Science for Programs/Policies and Interpretation of Research Evidence (INSPIRE) Meeting CY NOV 28-30, 2012 CL Natl Inst Hlth, Bethesda, MD HO Natl Inst Hlth DE diet; metagenomics; microbial-host co-metabolism; microbiome; probiotics/prebiotics ID HUMAN-MILK OLIGOSACCHARIDES; IMMUNE-SYSTEM; NUTRITIONAL MODULATION; INTESTINAL MICROBIOTA; HOST METABOLISM; DISEASE RISK; OBESITY; PROBIOTICS; EVOLUTION; HEALTH AB Homo sapiens harbor trillions of microbes, whose microbial metagenome (collective genome of a microbial community) using omic validation interrogation tools is estimated to be at least 100-fold that of human cells, which comprise 23,000 genes. This article highlights some of the current progress and open questions in nutrition-related areas of microbiome research. It also underscores the metabolic capabilities of microbial fermentation on nutritional substrates that require further mechanistic understanding and systems biology approaches of studying functional interactions between diet composition, gut microbiota, and host metabolism. Questions surrounding bacterial fermentation and degradation of dietary constituents (particularly by Firmicutes and Bacteroidetes) and deciphering how microbial encoding of enzymes and derived metabolites affect recovery of dietary energy by the host are more complex than previously thought. Moreover, it is essential to understand to what extent the intestinal microbiota is subject to dietary control and to integrate these data with functional metabolic signatures and biomarkers. Many lines of research have demonstrated the significant role of the gut microbiota in human physiology and disease. Probiotic and prebiotic products are proliferating in the market in response to consumer demand, and the science and technology around these products are progressing rapidly. With high-throughput molecular technologies driving the science, studying the bidirectional interactions of host-microbial cometabolism, epithelial cell maturation, shaping of innate immune development, normal vs. dysfunctional nutrient absorption and processing, and the complex signaling pathways involved is now possible. Substantiating the safety and mechanisms of action of probiotic/prebiotic formulations is critical. Beneficial modulation of the human microbiota by using these nutritional and biotherapeutic strategies holds considerable promise as next-generation drugs, vaccinomics, and metabolic agents and in novel food discovery. C1 [Duffy, Linda C.] Natl Ctr Complementary & Integrat Hlth, NIH, US Dept HHS, Bethesda, MD 20892 USA. [Raiten, Daniel J.] Eunice Kennedy Shriner Natl Inst Child Hlth & Hum, NIH, US Dept HHS, Bethesda, MD USA. [Hubbard, Van S.] NIH, US Dept HHS, Div Nutr Res Coordinat, Bethesda, MD USA. [Starke-Reed, Pamela] ARS, USDA, Beltsville, MD USA. RP Duffy, LC (reprint author), Natl Ctr Complementary & Integrat Hlth, NIH, US Dept HHS, Bethesda, MD 20892 USA. EM duffyl@mail.nih.gov NR 81 TC 8 Z9 8 U1 5 U2 59 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2015 VL 145 IS 5 SU S BP 1123S EP 1130S DI 10.3945/jn.114.194936 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH4OP UT WOS:000354013100004 PM 25833886 ER PT J AU Aldema-Ramos, ML Muir, ZE Uknalis, J Truong, N Trusello, J AF Aldema-Ramos, M. L. Muir, Z. E. Uknalis, J. Ngoan Truong Trusello, J. TI DEVELOPMENT OF AN ALTERNATIVE Low SALT BOVINE HIDE PRESERVATION USING PEG AND CRUDE GLYCEROL, PART II: MECHANICAL PROPERTIES OF LEATHER PRODUCTS SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article AB To obtain good quality leather products, the hides from which they are derived should be preserved properly while in storage and transit to prevent putrefaction. Current practice for hide preservation is salt curing via concentrated NaCl solutions. The objective of the current study is to develop alternative brining processes that require lesser amounts of salt and have no adverse effect on the resulting leather products. By incorporating a dehydrating agent such as polyethylene glycol polymers (PEG), only a fraction of the commonly used amount of salt is necessary (from about 50% to less than a 25% of the weight of raw hide) to generate an efficiently preserved hide. The scanning electron microscopic (SEM) images and mechanical properties of the resulting crust leather products were comparable to the control obtained from traditionally preserved hides. To compensate for the potential over drying and formation of very tight grain, a humectant such as glycerol (with sodium carbonate) was also included. The rehydration of the test preserved hides is completed in a shorter period of time because the amount of salt in the hides is already quite low. Considering the low cost in obtaining the crude glycerol and its positive effects on quality of leather, it is quite desirable as an ingredient in the new alternative less salt hide preservation. C1 [Aldema-Ramos, M. L.; Muir, Z. E.; Uknalis, J.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Ngoan Truong; Trusello, J.] Drexel Univ, Philadelphia, PA 19104 USA. RP Aldema-Ramos, ML (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM mila.ramos@ars.usda.gov NR 10 TC 0 Z9 0 U1 1 U2 7 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD MAY PY 2015 VL 110 IS 5 BP 125 EP 129 PG 5 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA CI0JA UT WOS:000354421700001 ER PT J AU Ellis, MM Ivan, JS Tucker, JM Schwartz, MK AF Ellis, Martha M. Ivan, Jacob S. Tucker, Jody M. Schwartz, Michael K. TI rSPACE: Spatially based power analysis for conservation and ecology SO METHODS IN ECOLOGY AND EVOLUTION LA English DT Article DE detection probability; occupancy estimation; population monitoring; population trends; power analysis; sampling design; spatial simulation ID IMPERFECT DETECTION; PRESENCE-ABSENCE; OCCUPANCY; POPULATIONS; CONSTRAINTS; WOLVERINE; ANIMALS; DESIGN AB Power analysis is an important step in designing effective monitoring programs to detect trends in plant or animal populations. Although project goals often focus on detecting changes in population abundance, logistical constraints may require data collection on population indices, such as detection/non-detection data for occupancy estimation. We describe the open-source R package, rSPACE, for implementing a spatially based power analysis for designing monitoring programs. This method incorporates information on species biology and habitat to parameterize a spatially explicit population simulation. A sampling design can then be implemented to create replicate encounter histories which are subsampled and analysed to estimate the power of the monitoring program to detect changes in population abundance over time, using occupancy as a surrogate. The proposed method and software are demonstrated with an analysis of wolverine monitoring in a U.S. Northern Rocky Mountain landscape. The package will be of use to ecologists interested in evaluating objectives and performance of monitoring programs. C1 [Ellis, Martha M.] US Forest Serv, USDA, Rocky Mt Res Stn, Bozeman, MT 59715 USA. [Ivan, Jacob S.] Colorado Pk & Wildlife, Wildlife Res Ctr, Ft Collins, CO 80526 USA. [Tucker, Jody M.] US Forest Serv, USDA, Pacific Southwest Reg, Sequoia Natl Forest, Porterville, CA 93257 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Ellis, MM (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Montana State Univ Campus, Bozeman, MT 59715 USA. EM martha.ellis@gmail.com RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU USFS; RMRS; PECASE award; USFWS Region 6 FX Initial funding for this project was provided by USFS, the RMRS, a PECASE award to M.K.S., and a Section 6 grant from USFWS Region 6 to Colorado Parks and Wildlife. We thank J. Laake, P. Lukacs and G. White for invaluable technical advice, and we are grateful to M. Alldredge, D. Tripp and two anonymous reviewers whose comments on earlier drafts greatly improved this article. NR 18 TC 2 Z9 2 U1 8 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2041-210X EI 2041-2096 J9 METHODS ECOL EVOL JI Methods Ecol. Evol. PD MAY PY 2015 VL 6 IS 5 BP 621 EP 625 DI 10.1111/2041-210X.12369 PG 5 WC Ecology SC Environmental Sciences & Ecology GA CI1YI UT WOS:000354540700014 ER PT J AU Cook, KL Ritchey, EL Loughrin, JH Haley, M Sistani, KR Bolster, CH AF Cook, K. L. Ritchey, E. L. Loughrin, J. H. Haley, M. Sistani, K. R. Bolster, C. H. TI Effect of turning frequency and season on composting materials from swine high-rise facilities SO WASTE MANAGEMENT LA English DT Article DE Compost; Nitrogen; Nitrifier; Swine; Slurry; Waste management ID AMMONIA-OXIDIZING ARCHAEA; CATTLE FEEDLOT MANURE; PIG SLURRY; LAND APPLICATION; MASS-LOSS; NITROGEN; BACTERIA; SOIL; GENES; SCALE AB Composting swine slurries has several advantages, liquid slurries are converted to solids at lower moisture, the total volume and weight of material is reduced and the stabilized product is more easily transported off-site. Despite this, swine waste is generally stored, treated and applied in its liquid form. High-rise finishing facilities (HRFF) permit liquid slurries to be converted to solids which are partially decomposed underneath the HRFF and then finished in compost windrows. The purpose of this study was to evaluate the effect of turning frequency and ambient weather conditions on biological, physical and chemical properties of composted slurry-woodchip mixtures from HRFF. Compost trials were conducted in either fall (FT) or spring (ST) and piles were turned once or three times per week or upon compost temperature reaching 65 degrees C. Physical, chemical and microbiological characteristics were measured over the course of 112 (FT) or 143 (ST) days of composting. Total carbon, total nitrogen (N) and inorganic N decreased in all piles. Ammonium decreased while nitrate increased in all piles (including unturned), but total N losses were greatest in piles turned more frequently during the ST. Microbial populations of nitrifiers were dominated by ammonia-oxidizing archaea (3.0 x 10(3)-4.2 x 10(6) cells g(-1) compost) but ammonia oxidizing bacteria (below detection to 6.0 x 10(5) cells g(-1) compost) varied in response to turning and compost temperature; denitrifiers were present in high concentrations throughout the process. Swine HRFF materials composted well in windrows regardless of turning frequency and despite significant differences in starting materials and low initial C/N. Volume reduction, low moisture and low readily degradable organic matter suggest that the finished compost would have lower transportation costs and should provide value as a soil conditioner. Published by Elsevier Ltd. C1 [Cook, K. L.; Loughrin, J. H.; Haley, M.; Sistani, K. R.; Bolster, C. H.] USDA ARS, Food Anim Environm Syst Res Unit, Bowling Green, KY USA. [Ritchey, E. L.] Univ Kentucky, Plant & Soil Sci, Lexington, KY USA. RP Cook, KL (reprint author), 230 Bennett Lane, Bowling Green, KY 42104 USA. EM kim.cook@ars.usda.gov; Edwin.ritchey@uky.edu; john.loughrin@ars.usda.gov; marty.haley@ky.usda.gov; karamat.sistani@ars.usda.gov; carl.bolster@ars.usda.gov FU USDA-ARS [214, CRIS 6445-12630-004-00D] FX The authors thank Stacy Antle, Derrek Brown, Rohan Parekh and Jason Simmons for valuable technical assistance. The authors also thank O'Bryan Grain Farms. Inc. for providing access to facilities, materials, and equipment in support of this experiment. This research was conducted as part of USDA-ARS National Program 214: Agricultural and Industrial By-products: CRIS 6445-12630-004-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 54 TC 5 Z9 5 U1 1 U2 29 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0956-053X J9 WASTE MANAGE JI Waste Manage. PD MAY PY 2015 VL 39 BP 86 EP 95 DI 10.1016/j.wasman.2015.02.019 PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CH6NC UT WOS:000354152300011 PM 25752584 ER PT J AU Peng, XL Maharjan, B Yu, CL Su, AY Jin, V Ferguson, RB AF Peng, Xianlong Maharjan, Bijesh Yu, Cailian Su, Anyu Jin, Virginia Ferguson, Richard B. TI A Laboratory Evaluation of Ammonia Volatilization and Nitrate Leaching following Nitrogen Fertilizer Application on a Coarse-Textured Soil SO AGRONOMY JOURNAL LA English DT Article ID SURFACE-APPLIED UREA; NITRIFICATION INHIBITORS; RELEASE UREA; TILL CORN; N LOSSES; CROP; MAIZE; MANAGEMENT; SUMMER; FORMS AB In a series of field studies, differing rainfall patterns within the first month after N fertilizer application to a coarse-textured soil significantly affected yields and N-use efficiency of irrigated corn (Zea mays L.), and responses varied with N source. A laboratory study was conducted to evaluate effects of N source with precipitation following N application to a coarse-textured soil. Nitrogen sources included urea-ammonium nitrate solution (UAN), UAN with additives of either nitrapyrin (2-chloro-6-[trichloromethyl]pyridine) as a nitrification inhibitor or maleic-itaconic acid copolymer as a urease and nitrification inhibitor, or polymer-coated dry urea (PCU). These products were applied to soil in chambers from which ammonia (NH3) volatilization and nitrate (NO3-) leaching were measured over 31 d following fertilization. Precipitation events simulated rainfall frequencies and amounts that occurred in field studies in dry and wet conditions. Ammonia volatilization was lower in wet than dry conditions. Total NH3 loss for the dry precipitation regime ranged from 11 to 18% of applied N fertilizer for all treatments except PCU (<1%). In contrast, all treatments in wet conditions had low NH3 loss (<1% of applied N). However, substantial NO3- leaching occurred with wet conditions, comprising 48 to 66% of applied N for most treatments. Leaching loss was the greatest for UAN, followed by UAN with additives. For either dry or wet environments, losses of N from PCU to either NH3 volatilization or NO3- leaching were negligible. C1 [Peng, Xianlong; Su, Anyu] Northeast Agr Univ, Resource & Environm Coll, Harbin 150030, Peoples R China. [Maharjan, Bijesh; Ferguson, Richard B.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Yu, Cailian] Harbin Univ Sci & Technol, Inst Chem & Environm Engn, Harbin 150040, Peoples R China. [Jin, Virginia] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Maharjan, B (reprint author), Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. EM bmaharjan@unl.edu NR 47 TC 3 Z9 4 U1 11 U2 62 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 871 EP 879 DI 10.2134/agronj14.0537 PG 9 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600006 ER PT J AU Thompson, NM Larson, JA Lambert, DM Roberts, RK Mengistu, A Bellaloui, N Walker, ER AF Thompson, Nathanael M. Larson, James A. Lambert, Dayton M. Roberts, Roland K. Mengistu, Alennu Bellaloui, Nacer Walker, Eric R. TI Mid-South Soybean Yield and Net Return as Affected by Plant Population and Row Spacing SO AGRONOMY JOURNAL LA English DT Article ID WEED MANAGEMENT-SYSTEMS; SEEDING RATE; ECONOMIC-ANALYSIS; USA; GROWTH; RESPONSES; DATES AB Traditionally grown soybean [Glycine max (L.) Merr.] Maturity Groups V and VI are subject to late-season drought in the US Mid-South, resulting in yield reductions when planted in mid-May. Earlier maturing soybean, such as the more recently adapted Maturity Group III cultivars, have generated interest among farmers as a way to avoid the effects of late-season drought. We investigated economically optimal plant population density for soybean considering seeding rate, row spacing, seed and soybean prices, and weather for Maturity Groups V, IV, and III grown on the rainfed soils in the rolling uplands region of the US Mid-South. Three separate experiments were conducted for Maturity Groups V, IV, and III in 2005 through 2007. Soybean was planted in mid-May in narrow (38-cm) and wide (76-cm) rows at seeding rates of 60 to 593 (x 10(3)) seeds ha(-1). Three key implications resulted from this study. First, yield did not respond to plant population density, and an extended yield plateau was found in each maturity group experiment, implying an economic incentive to reduce seed costs to achieve the plant population density at the minimum point on the yield plateau. Second, yield and net-return responses to row spacing were inconsistent and primarily influenced by weather, but if Maturity Group III soybean were planted, narrow row spacing may provide higher expected yields and net returns. Third, planting Maturity Group III soybean may not overcome the susceptibility of Maturity Groups V and IV soybean to the late-season drought conditions in the rolling uplands region of the Mid-South. C1 [Thompson, Nathanael M.] Oklahoma State Univ, Dept Agr Econ, Stillwater, OK 74078 USA. [Larson, James A.; Lambert, Dayton M.; Roberts, Roland K.] Univ Tennessee, Dept Agr & Resource Econ, Knoxville, TN 37996 USA. [Mengistu, Alennu] USDA ARS, Crop Genet Res Unit, Jackson, TN 38301 USA. [Bellaloui, Nacer] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Walker, Eric R.] Univ Tennessee, Dept Plant & Soil Sci, Highland Rim Res & Educ Ctr, Springfield, TN 37172 USA. RP Larson, JA (reprint author), Univ Tennessee, Dept Agr & Resource Econ, Knoxville, TN 37996 USA. EM jlarson2@utk.edu FU United State Department of Agriculture (USDA), Agricultural Research Service [6402-21220-011-00D]; USDA, Cooperative State Research, Education, and Extension Service through Tennessee Hatch Project [TEN00442] FX We thank Chris Street, USDA-ARS South East Area, Biological Science Technician, for his assistance in maintaining plots and collecting data for the test. This research was funded by the United State Department of Agriculture (USDA), Agricultural Research Service project number 6402-21220-011-00D and USDA, Cooperative State Research, Education, and Extension Service through Tennessee Hatch Project TEN00442. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by USDA. NR 52 TC 1 Z9 1 U1 3 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 979 EP 989 DI 10.2134/agronj14.0453 PG 11 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600019 ER PT J AU Nielsen, DC Lyon, DJ Hergert, GW Higgins, RK Calderon, FJ Vigil, MF AF Nielsen, David C. Lyon, Drew J. Hergert, Gary W. Higgins, Robert K. Calderon, Francisco J. Vigil, Merle F. TI Cover Crop Mixtures Do Not Use Water Differently than Single-Species Plantings SO AGRONOMY JOURNAL LA English DT Article ID SOIL-WATER; WINTER-WHEAT; YIELD; BENEFITS; TILLAGE AB Recent recommendations advocating the use of cover crop mixtures instead of single-species in semi-arid environments require rigorous scientific studies. One of those stated benefits is greatly reduced water use by cover crops grown in mixtures. The objectives of this study were to characterize soil water extraction patterns and determine water use of cover crops grown in single-species plantings and in a 10-species mixture and to compare cover crop water use to evaporative water loss from no-till fallow. The study was conducted at Akron, CO, and Sidney, NE, during the 2012 and 2013 growing seasons on silt loam soils. At each location there were a dryland treatment and an irrigated treatment. Soil water contents were measured by scattering and time-domain reflectometry at six depths (0.0-1.8 in, Akron) or four or five depths (to 1.2 m or 13 in, Sidney). There were no consistent significant differences in soil water contents or growing season crop water use with the single-species plantings compared with the 10-species mixture. Cover crop water use (216 mm) averaged 1.78 times greater than evaporative water loss (122 mm) from the no-till fallow treatment with proso millet (Panicum miliaceum L.) residue, There appears to be no evidence from data collected in this semi-arid environment, even when irrigated to simulate higher rainfall environments, to support the conclusion that cover crops grown in multi-species mixtures use water differently than single species-plantings of cover crops. C1 [Nielsen, David C.; Calderon, Francisco J.; Vigil, Merle F.] ARS, USDA, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Lyon, Drew J.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Hergert, Gary W.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. [Higgins, Robert K.] Univ Nebraska, High Plains Ag Lab, Sidney, NE 69162 USA. RP Nielsen, DC (reprint author), ARS, USDA, Cent Great Plains Res Stn, 40335 Cty Rd GG, Akron, CO 80720 USA. EM david.nielsen@ars.usda.gov RI Nielsen, David/A-8044-2009 OI Nielsen, David/0000-0002-8240-7183 NR 15 TC 7 Z9 6 U1 5 U2 34 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1025 EP 1038 DI 10.2134/agronj14.0504 PG 14 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600025 ER PT J AU Long, DS Whitmus, JD Engel, RE Brester, GW AF Long, Dan S. Whitmus, Jeffrey D. Engel, Richard E. Brester, Gary W. TI Net Returns from Terrain-Based Variable-Rate Nitrogen Management on Dryland Spring Wheat in Northern Montana SO AGRONOMY JOURNAL LA English DT Article ID FERTILIZER APPLICATION; ECONOMIC-FEASIBILITY; SPATIAL VARIABILITY; HUMMOCKY TERRAIN; SOIL PROPERTIES; YIELD; EROSION; ZONES; WATER; GRAIN AB Agricultural producers can use variable-rate application technology to vary N fertilizer within fields. This study was conducted to estimate changes in net returns from implementation of variable-rate N management (VNM) on hard red spring wheat (Triticum aestivum L.) in a summer-fallow region in northern Montana. Net return from uniform N management (UNM) traditionally used by producers was compared with that from VNM in eight dryland fields between 1994 and 2004. Field experiments consisted of a replicated series of four to six N rates applied within strips oriented with the length of each field. Management zones (MZs) were created by dividing the fields into upper slopes, south-facing middle slopes, north-facing middle slopes, and lower slopes. Nitrogen recommendations for MZs were based on soil N testing and expected yields. Grain yield data were obtained using a production-size combine equipped with a yield monitor. Mean grain protein and yield were similar between VNM and UNM. Yield differences were <223 kg ha(-1) and averaged only 18 kg ha(-1). Grain yield did not differ significantly among N rates within MZs. In seven of the eight sites, net returns from VNM were up to US$27.97 ha(-1) less than from UNM and were not profitable if Environmental quality Incentive Program payments of US$6.36 ha(-1) were considered as part of net income. Little evidence existed that VNM based on constructed MZs and predetermined N recommendations improves grain yields and profits or reduces N use in water-limited, summer-fallow systems of northern Montana, C1 [Long, Dan S.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. [Whitmus, Jeffrey D.; Engel, Richard E.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Brester, Gary W.] Montana State Univ, Dept Agr Econ & Econ, Bozeman, MT 59717 USA. RP Long, DS (reprint author), USDA ARS, Columbia Plateau Conservat Res Ctr, POB 370, Pendleton, OR 97801 USA. EM dan.long@ars.usda.gov FU Montana Wheat and Barley Committee; Montana Fertilizer Tax Fund Advisory Committee; Westco Fertilizers; USDA-IFAFS [2001-52103-11321] FX The authors acknowledge the Montana Wheat and Barley Committee, Montana Fertilizer Tax Fund Advisory Committee, Westco Fertilizers, and USDA-IFAFS (Grant no. 2001-52103-11321) for external funding support, and Willard and Karla Vaughn, Mark and Nancy Peterson, Ed Bumgarner, Les and Terry Kaercher, Karl and Bud Mavencamp, and Wes Anderson for access to their fields. The technical assistance of Gregg R. Carlson, Terry Grass, and Tom Allen is also appreciated. Special thanks to Dr. David Buschena in the planning of the experimental design and economic analysis. NR 38 TC 0 Z9 0 U1 3 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1055 EP 1067 DI 10.2131/agronj11.0331 PG 13 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600028 ER PT J AU Gesch, RW Johnson, JMF AF Gesch, Russ W. Johnson, Jane M. -F. TI Water Use in Camelina-Soybean Dual Cropping Systems SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN CORN-BELT; WINTER CAMELINA; GREAT-PLAINS; SOWING DATE; WHEAT; YIELD; IRRIGATION; OILSEED; CUPHEA; CROPS AB Global population growth is increasing the demand for food, feed, fiber, and fuel. Recently, we reported that winter camelina (('amelina saliva L.) can be feasibly double- (DC) and relay-cropped with soybean I Glycine max (L.) Merr.] in the northern Corn Belt region as an option for sustainably intensified crop production. However, water availability can be a limiting factor for dual crop systems. Therefore, the present study determined seasonal water use in various winter camelina soybean dual crop systems that included two sequential DC and two relay-crop treatments compared with a full-season monocropped soybean (Mono-Soy). The rooting characteristics of camelina were also evaluated. The study was conducted on a Barnes loam soil (fine-loamy, mixed, superactive, frigid Calcic Hapludoll) in west central Minnesota over two growing seasons in 2010 and 2011. Camelina had a meager root system with 82% fits root density found in the top 0.3 in ofsoil. Although both DC and relay-crop treatments used more seasonal water than a sole soybean crop, on average it was only 26 and 50 min greater in 2010 and 2011, respectively. Camelina is a low water user compared with soybean. Total cropping system water use efficiency for relay-cropping was comparable to the sole soybean crop, but for the DC treatments was less. This cropping strategy is a way to sustainably integrate food and fuel production to meet societal needs of a growing population. Moreover, our results indicate that it may be amenable to most rainfed-farming areas in the upper Midwest where soybean is grown. C1 [Gesch, Russ W.; Johnson, Jane M. -F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Gesch, RW (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. EM russ.gesch@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 NR 26 TC 4 Z9 4 U1 5 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1098 EP 1104 DI 10.2134/agronj14.0626 PG 7 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600032 ER PT J AU Kimball, BA AF Kimball, Bruce A. TI Using Canopy Resistance for Infrared Heater Control When Warming Open-Field Plots SO AGRONOMY JOURNAL LA English DT Article ID SPRING WHEAT; TEMPERATURE; PERFORMANCE; EVAPORATION; ARRAYS; DATES AB To simulate future global warming, several research groups use arrays of infrared heaters to warm open-field plots with a control strategy that involves maintaining a constrat rise in canopy temperatures of the Heated plots above those of un-heated Reference plots. However, if the warming treatment itself alters canopy architecture, plant physiology, albedo or other plot characteristics in ways that affect the energy balances of the heated plots so as to make them materially different from those of the Reference plots, then the Reference plots no longer are proper references against which to determine a desired canopy temperature rise. Herein, a novel alternative control strategy is proposed whereby plant and air temperature and other micro-climate measurements are used to determine the canopy resistances of the Heated plots, which in turn are used to calculate the amount of infrared radiation that is needed to achieve the desired rise in canopy temperature. Then the voltage supplied to the heaters can be modulated accordingly to attain this temperature rise. C1 ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Kimball, BA (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM bruce.kimball@ars.usda.gov NR 25 TC 0 Z9 0 U1 6 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1105 EP 1112 DI 10.2134/agronj14.0418 PG 8 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600033 ER PT J AU Northup, BK Rao, SC AF Northup, Brian K. Rao, Srinivas C. TI Green Manure and Forage Potential of Lab lab in the US Southern Plains SO AGRONOMY JOURNAL LA English DT Article ID WARM-SEASON LEGUMES; GREAT-PLAINS; NUTRITIVE-VALUE; WINTER-WHEAT; QUALITY; GROWTH; YIELD; CAPABILITIES; PERFORMANCE; BIOMASS AB Forages currently available to support yearling stocker cattle in the U.S. southern Great Plains (SGP) frequently have low quality during mid-July through September. This study tested the tropical/subtropical legume labial-, [LabLzbpurpureus (L.) Sweet] as both green manure and forage in central Oklahoma. We compared biomass production and nutritive value of lablab (cultivar Rio Verde) to soybean [Glycine max (L.) Merr.] grown during fallow periods of conventional and no-till wheat (Triticum aestivurn L. em. Thell) in 2008 to 2011. Biomass was collected at different growth stages during growing seasons and assayed for yield, N accumulation (kg N ha(-1)), crude protein (CP), and in vitro digestible dry matter (IVDDNI) concentration. Lablab produced more biomass than soybean (1415 vs. 1170 kg ha(-1) yr(-1), P = 0.02) across tillage systems; production declined over years < 0.01). Legume x year interaction (P <= 0.03) was noted in CP and IVDDNI. Lablab averaged (+/- 1 SD) more accumulated N than soybean 155(+/- 25) vs. 40(+/- 24) kg N ha(-1) yr(-1)] and had greater CP (P < 0.01) in all years; amounts in both species declined with length of study. Digestibility oflablab exceeded soybean (P < 0.01) throughout the study. We concluded that labial-, contributed more biomass and N than soybean as green manure or forage within continuous systems of wheat production. Function of both legumes was limited by low precipitation and continuous double-cropping within wheat systems. Despite low production, lablab could be high quality forage for stocker cattle during late summer. C1 [Northup, Brian K.; Rao, Srinivas C.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Northup, BK (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM brian.northup@ars.usda.gov NR 40 TC 2 Z9 2 U1 2 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1113 EP 1118 DI 10.2134/agronj14.0455 PG 6 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600034 ER PT J AU Adeli, A Read, JJ McCarty, J Jenkins, JN Feng, G AF Adeli, Ardeshir Read, John J. McCarty, Jack Jenkins, Johnie N. Feng, Gary TI Soybean Yield and Nutrient Utilization following Long-Term Pelletized Broiler Litter Application to Cotton SO AGRONOMY JOURNAL LA English DT Article ID DAIRY-CATTLE MANURE; POULTRY LITTER; COMPOST APPLICATIONS; SOIL PROPERTIES; SUBSURFACE APPLICATION; CORN PRODUCTION; NITROGEN; AVAILABILITY; PHOSPHORUS; GROWTH AB Broiler (Gallus gallus domestic-us) litter may have long-lasting plant growth benefits after application is terminated. This study determined residual effects of pelletized litter applied to cotton (Gossypium hirsuium L.) on yield and nutrient utilization of soybean [Glycine max (L.) Merr.]. Treatments were replicated three times. Treatments included pelletized broiler litter subsurface banded to cotton at the rate of 6.7 Mg ha(-1), urea-ammonium nitrate (UAN solution) injected at the rate of 134 kg N ha(-1) and unfertilized control in three previous years (2008-2010). Soybean planted in 2011, 2012, and 2013, and provided no additional litter or fertilizer. Soybean height was determined at 28, 42, 56, -1,86, 102, and 115 d after planting. Total aboveground biomass, leaf area index (LAI) and plant nutrient concentrations were determined at growth stage R5. Soybean yield and plant nutrient concentrations were greater in pelletized litter than fertilizer N treatments. Soybean plants in plots received pelletized litter were significantly (P < 0.05) taller and had greater leaf area than the fertilizer N and control plots. Total aboveground biomass at R5 did not differ between pelletized litter and fertilizer N treatments; however grain yield was greatest for residual litter treatment. In 2011, soybean grain yield and grain N, P. and K uptake were significantly greater in plots received pelletized litter, as compared with fertilizer N and control plots. Residual effects of pelletized broiler litter in cotton increased soybean production for 1 yr and influenced soil fertility for several years beyond the year of application. C1 [Adeli, Ardeshir; Read, John J.; McCarty, Jack; Jenkins, Johnie N.; Feng, Gary] USDA ARS, Mississippi State, MS 39762 USA. RP Adeli, A (reprint author), USDA ARS, Mississippi State, MS 39762 USA. EM ardeshir.adeli@ars.usda.gov NR 27 TC 1 Z9 1 U1 2 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1128 EP 1134 DI 10.2134/agronj140497 PG 7 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600036 ER PT J AU Lehrsch, GA Brown, B Lentz, RD Johnson-Maynard, JL Leytem, AB AF Lehrsch, G. A. Brown, B. Lentz, R. D. Johnson-Maynard, J. L. Leytem, A. B. TI Compost and Manure Effects on Sugarbeet Nitrogen Uptake, Nitrogen Recovery, and Nitrogen Use Efficiency SO AGRONOMY JOURNAL LA English DT Article ID CATTLE FEEDLOT MANURE; CORN PRODUCTION; ORGANIC-MATTER; SOIL; FERTILIZER; YIELD; NUTRIENT; MINERALIZATION; AVAILABILITY; PHOSPHORUS AB To maximize recoverable sucrose from sugarbeet (Beta vulgaris L.), producers must effectively manage added N, be it from urea or organic sources such as manure or composted manure. Our study's objective was to determine the effects of a one-time application of stockpiled and composted dairy cattle (Bos taurus) manure on sugarbeet N uptake, nitrogen recovery (NR) and nitrogen use efficiency (NUE). First-year Site A treatments included a control (no N), urea (202 kg N ha(-1)), compost (218 and 435 kg estimated available N ha I), and manure (140 and 280 kg available N ha 1), Site B treatments were a control, urea (82 kg N ha(-1)), compost (81 and 183 kg available N ha(-1)), and manure (1-3 and 340 kg available N ha-1). Compost and manure were incorporated into two silt barns, a Greenleaf (fine-silty, mixed, superactive, mesic Xeric Calciargid) near Parma, ID), in EA 2002 and 2003 and a Portneuf (coarse-silty, mixed, superactive, mesic Durinodic Xeric Haplocalcid) near Kimberly, ID, in fall 2002 with sugarbeet planted the following spring. At each site, N uptake of sugarbect tops, but not roots, was similar whether fertilized with urea or organic N, regardless of rate. Incorporating equal organic amendment rates to 0.05 rather than 0.10 m increased whole-plant N uptake 1.13-fold, to 163.3 kg N ha(-1). In general, NR varied among fertilizer sources such that urea >> manure > compost. Where similar available N rates were supplied, NUE ranged from 44.1 to 83.5 kg sucrose kg(-1) available N, not differing among inorganic and organic N sources within site-years. C1 [Lehrsch, G. A.; Lentz, R. D.; Leytem, A. B.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Brown, B.] Univ Idaho, Parma Res & Extens Ctr, Parma, ID 83660 USA. [Johnson-Maynard, J. L.] Univ Idaho, PSES Agr Sci, Moscow, ID 83844 USA. RP Lehrsch, GA (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM Gary.Lehrsch@ars.usda.gov NR 55 TC 4 Z9 4 U1 4 U2 29 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 1155 EP 1166 DI 10.2134/agronj14.0507 PG 12 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600039 ER PT J AU Ross, AB Kristensen, M Seal, CJ Jacques, P McKeown, NM AF Ross, Alastair B. Kristensen, Mette Seal, Chris J. Jacques, Paul McKeown, Nicola M. TI Recommendations for reporting whole-grain intake in observational and intervention studies SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE whole grain; intake; intervention studies; epidemiology; bran; germ ID CARDIOVASCULAR-DISEASE; HEALTH CLAIMS; DIETARY INTERVENTION; GLYCEMIC RESPONSE; CEREAL FIBER; RISK; CONSUMPTION; WOMEN; WHEAT; BRAN AB The finding that people who eat the most whole grains have a lower risk of cardiovascular disease and diabetes, compared with those who eat the least, is one of the most consistent findings in nutritional epidemiology. However, criteria for reporting whole-grain intake have varied widely, making it difficult to precisely explore the relation of whole grains and grain components with health outcomes. To enable better understanding of the health benefits of whole grain rich diets, we propose that both observational and intervention studies should as far as possible be required to report as follows when describing whole grains: 1) quantify the amount of whole grain in the food or product in grams on a dry-weight basis, 2) describe the whole-grain definition used, 3) report and separate the different types of grains used, 4) if possible, report the structure of the grains (intact, crushed, partially milled) in foods, and 5) describe the main types of products used and processes used to make them. Added bran and germ should be reported distinctly from whole grains. In addition, we strongly recommend the incorporation of biomarkers of whole-grain intake to check compliance to intervention diets and help attenuate for errors in dietary recall of whole-grain intake. Of these measures, reporting whole-grain intake in grams is essential for future research work in the area. Improving reporting and estimation of whole-grain intake will enable easier comparison between different studies and lead to stronger meta-analyses in the future. C1 [Ross, Alastair B.] Chalmers, Dept Biol & Biol Engn, Food & Nutr Sci, S-41296 Gothenburg, Sweden. [Kristensen, Mette] Univ Copenhagen, Dept Nutr Exercise & Sports, Copenhagen, Denmark. [Seal, Chris J.] Newcastle Univ, Human Nutr Res Ctr, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Jacques, Paul; McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Dept, Boston, MA 02111 USA. RP Ross, AB (reprint author), Chalmers, Dept Biol & Biol Engn, Food & Nutr Sci, S-41296 Gothenburg, Sweden. EM alastair.ross@chalmers.se RI Kristensen, Mette/A-4219-2011; OI Kristensen, Mette/0000-0002-0065-8174; Ross, Alastair/0000-0002-9585-0141 FU Cereal Partners Worldwide; General Mills Bell Institute of Health and Nutrition FX The authors' contributions were as follows-ABR: drafted the manuscript with substantial scientific input from all coauthors; and all authors: read and approved the final draft of the manuscript. ABR has performed consulting work for Nestec SA (the research arm of the Nestle food and beverage company) and Cereal Partners Worldwide. MK has performed consulting work for Cereal Partners Worldwide. CJS has received unrestricted research funds for investigator-led studies from Cereal Partners Worldwide. PJ is a member of the Bay State Milling Nutrition and Science Advisory Council and the Grains for Health Foundation Scientific Advisory Committee. NMM has received funding for investigator-initiated grants from the General Mills Bell Institute of Health and Nutrition and is a scientific advisor for the Whole Grains Council. No consulting work undertaken by any author is in any manner related to the present work. NR 40 TC 18 Z9 18 U1 1 U2 22 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2015 VL 101 IS 5 BP 903 EP 907 DI 10.3945/ajcn.114.098046 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH5KS UT WOS:000354075100004 PM 25809851 ER PT J AU Maalouf, J Cogswell, ME Yuan, K Martin, C Gunn, JP Pehrsson, P Merritt, R Bowman, B AF Maalouf, Joyce Cogswell, Mary E. Yuan, Keming Martin, Carrie Gunn, Janelle P. Pehrsson, Pamela Merritt, Robert Bowman, Barbara TI Top sources of dietary sodium from birth to age 24 mo, United States, 2003-2010 SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE children; infants; nutrition; sodium; birth; foods; toddler ID BLOOD-PRESSURE TRENDS; NON-HISPANIC INFANTS; FEEDING INFANTS; US INFANTS; CHILDREN; CHILDHOOD; TODDLERS; METAANALYSIS; ADOLESCENTS; CONSUMPTION AB Background: Sodium intake is high in US children. Data are limited on the dietary sources of sodium, especially from birth to age 24 mo. Objective: We identified top sources of dietary sodium in US children from birth to age 24 mo. Design: Data from the NHANES 2003-2010 were used to examine food sources of sodium (population proportions and mean intakes) in 778 participants aged 0-5.9 mo, 914 participants aged 6-11.9 mo, and 1219 participants aged 12-23.9 mo by sociodemographic characteristics. Results: Overall, mean dietary sodium intake was low in 0-5.9-mo-old children, and the top contributors were formula (71.7%), human milk (22.9%), and commercial baby foods (2.2%). In infants aged 6-11.9 mo, the top 5 contributors were formula (26.7%), commercial baby foods (8.8%), soups (6.1%), pasta mixed dishes (4.0%), and human milk (3.9%). In children aged 12-23.9 mo, the top contributors were milk (12.2%), soups (5.4%), cheese (5.2%), pasta mixed dishes (5.1%), and frankfurters and sausages (4.6%). Despite significant variation in top food categories across racial/ethnic groups, commercial baby foods were a top food contributor in children aged 6-11.9 mo, and frankfurters and sausages were a top food contributor in children aged 12-23.9 mo. The top 5 food categories that contributed to sodium intake also differed by sex. Most of the sodium consumed (83-90%) came from store foods (e.g., from the supermarket). In children aged 12-23.9 mo, 9% of sodium consumed came from restaurant foods, and 4% of sodium came from childcare center foods. Conclusions: The vast majority of sodium consumed comes from foods other than infant formula or human milk after the age of 6 mo. Although the majority of sodium intake was from store foods, after age 12 mo, restaurant foods contribute significantly to intake. Reducing the sodium content in these settings would reduce sodium intake in the youngest consumers. C1 [Maalouf, Joyce; Cogswell, Mary E.; Yuan, Keming; Gunn, Janelle P.; Merritt, Robert; Bowman, Barbara] CDC, Div Heart Dis & Stroke Prevent, Natl Ctr Chron Dis Prevent & Hlth Promot, Atlanta, GA 30333 USA. [Maalouf, Joyce] IHRC Inc, Atlanta, GA USA. [Martin, Carrie; Pehrsson, Pamela] ARS, USDA, Beltsville, MD USA. RP Maalouf, J (reprint author), 4770 Buford Highway Northeast,Mailstop F72, Atlanta, GA 30341 USA. EM vjh6@cdc.gov FU CDC FX Supported by the CDC. NR 40 TC 4 Z9 6 U1 2 U2 6 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2015 VL 101 IS 5 BP 1021 EP 1028 DI 10.3945/ajcn.114.099770 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CH5KS UT WOS:000354075100017 PM 25762806 ER PT J AU Liebman, K Swamidoss, I Vizcaino, L Lenhart, A Dowell, F Wirtz, R AF Liebman, Kelly Swamidoss, Isabel Vizcaino, Lucrecia Lenhart, Audrey Dowell, Floyd Wirtz, Robert TI The Influence of Diet on the Use of Near-Infrared Spectroscopy to Determine the Age of Female Aedes aegypti Mosquitoes SO AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE LA English DT Article ID INSECTICIDE RESISTANCE; CUTICULAR HYDROCARBONS; FIELD VALIDATION; DENGUE VECTOR; CULICIDAE; DIPTERA; POPULATIONS; MECHANISMS; TEMPERATURE; VIRUS AB Interventions targeting adult mosquitoes are used to combat transmission of vector-borne diseases, including dengue. Without available vaccines, targeting the primary vector, Aedes aegypti, is essential to prevent transmission. Older mosquitoes (>= 7 days) are of greatest epidemiological significance due to the 7-day extrinsic incubation period of the virus. Age-grading of female mosquitoes is necessary to identify post-intervention changes in mosquito population age structure. We developed models using near-infrared spectroscopy (NIRS) to age-grade adult female Ae. aegypti. To determine if diet affects the ability of NIRS models to predict age, two identical larval groups were fed either fish food or infant cereal. Adult females were separated and fed sugar water +/- blood, resulting in four experimental groups. Females were killed 1, 4, 7, 10, 13, or 16 days postemergence. The head/thorax of each mosquito was scanned using a near-infrared spectrometer. Scans from each group were analyzed, and multiple models were developed using partial least squares regression. The best model included all experimental groups, and positively predicted the age group (< or >= 7 days) of 90.2% mosquitoes. These results suggest both larval and adult diets can affect the ability of NIRS models to accurately assign age categories to female Ae. aegypti. C1 Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. USDA, Manhattan, KS USA. [Liebman, Kelly; Swamidoss, Isabel; Vizcaino, Lucrecia; Lenhart, Audrey; Wirtz, Robert] Ctr Dis Control & Prevent, CGH DPDM EB, Atlanta, GA 30329 USA. [Dowell, Floyd] USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Liebman, K (reprint author), Ctr Dis Control & Prevent, Div Parasit Dis & Malaria, Entomol Branch, 1600 Clifton Rd NE,MS-G49, Atlanta, GA 30329 USA. EM wuq4@cdc.gov; gtz8@cdc.gov; vtb6@cdc.gov; ajl8@cdc.gov; floyd.dowell@ars.usda.gov; bew5@cdc.gov NR 28 TC 1 Z9 1 U1 1 U2 6 PU AMER SOC TROP MED & HYGIENE PI MCLEAN PA 8000 WESTPARK DR, STE 130, MCLEAN, VA 22101 USA SN 0002-9637 EI 1476-1645 J9 AM J TROP MED HYG JI Am. J. Trop. Med. Hyg. PD MAY PY 2015 VL 92 IS 5 BP 1070 EP 1075 DI 10.4269/ajtmh.14.0790 PG 6 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA CH5KQ UT WOS:000354074900034 PM 25802436 ER PT J AU Roh, SG Carroll, JA Kim, SW AF Roh, Sang-Gun Carroll, Jeffery A. Kim, Sung Woo TI Effects of fermented soybean meal on innate immunity-related gene expressions in nursery pigs acutely challenged with lipopolysaccharides SO ANIMAL SCIENCE JOURNAL LA English DT Article DE fermented soybean meal; lipopolysaccharides; pig; plasma protein ID SPRAY-DRIED PLASMA; PITUITARY-ADRENAL AXIS; ACUTE-PHASE RESPONSE; GROWTH-PERFORMANCE; INTESTINAL MORPHOLOGY; WEANED PIGLETS; YOUNG-PIGS; INFLAMMATION; ACTIVATION; STRESS AB This experiment was to determine if replacing soybean meal with fermented soybean meal (FSBM) would reduce the innate immune response after lipopolysaccharide challenge and the changes of gene expression profiles associated with this response. Forty-eight 21 day-old pigs were housed individually and fed three diets for 15 days: CON (a diet without FSBM or spray-dried plasma protein; SDPP), PP7 (a diet with 7% SDPP), and FS10 (a diet with 10% FSBM). Pigs were fitted with a jugular vein catheters receiving lipopolysaccharide challenge (25g/kg body weight (BW)) on day 15. Blood was collected for 5h at 30-min intervals to measure cortisol. Expressions of gene transcripts in total RNA from leukocytes were compared using an oligonucleotide microarray at 210min after lipopolysaccharides injection. Cortisol of FS10 was lower (P<0.05) than CON after lipopolysaccharides challenge. The expression levels of 17 transcripts, including cytosolic glutathione peroxidase and glutathione S-transferase A4-4 were increased (P<0.05), whereas 23 genes including adiponectin, neonatal Fc receptor and tumor necrosis factor ligand superfamily member 5 were decreased (P<0.05) in FS10. This study suggests that FSBM-fed pigs can modulate expression of genes related to inflammatory response and anti-oxidant activity which can be a potential reason for reduced serum cortisol. C1 [Roh, Sang-Gun; Kim, Sung Woo] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Carroll, Jeffery A.] USDA ARS, Livestock Issue Res Unit, Lubbock, TX USA. [Roh, Sang-Gun] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Physiol, Sendai, Miyagi 980, Japan. RP Kim, SW (reprint author), N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. EM sungwoo_kim@ncsu.edu NR 40 TC 1 Z9 1 U1 2 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1344-3941 EI 1740-0929 J9 ANIM SCI J JI Anim. Sci. J. PD MAY PY 2015 VL 86 IS 5 BP 508 EP 516 DI 10.1111/asj.12319 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CH4VC UT WOS:000354030300004 PM 25440755 ER PT J AU Lee, KV Steinhauer, N Rennich, K Wilson, ME Tarpy, DR Caron, DM Rose, R Delaplane, KS Baylis, K Lengerich, EJ Pettis, J Skinner, JA Wilkes, JT Sagili, R vanEngelsdorp, D AF Lee, Kathleen V. Steinhauer, Nathalie Rennich, Karen Wilson, Michael E. Tarpy, David R. Caron, Dewey M. Rose, Robyn Delaplane, Keith S. Baylis, Kathy Lengerich, Eugene J. Pettis, Jeff Skinner, John A. Wilkes, James T. Sagili, Ramesh vanEngelsdorp, Dennis CA Bee Informed Partnership TI A national survey of managed honey bee 2013-2014 annual colony losses in the USA SO APIDOLOGIE LA English DT Article DE honey bee; survey; mortality; colony losses; USA ID WINTER LOSSES; UNITED-STATES; INFORMED PARTNERSHIP; VARROA-DESTRUCTOR; DECLINES; EUROPE; HEALTH; TRENDS; VIRUS AB Honey bee colony losses are a major concern in the USA and across the globe. Long-term data on losses are critical for putting yearly losses in context. US colony loss surveys have been conducted yearly since the winter of 2006-2007. Here, we report the results from the eighth annual survey on winter losses and the second annual survey of summer and annual losses. There were 7425 valid respondents (7123 backyard, 190 sideline, and 112 commercial beekeepers) managing 497,855 colonies, 19 % of the total US colonies. Total losses reported were 19.8 % [95 % CI 19.3-20.3 %] over the summer, 23.7 % [95 % CI 23.3-24.1 %] over the winter, and 34.1 % [95 % CI 33.6-34.6 %] for the whole year. Average losses were 15.1 % [95 % CI 14.5-15.7 %] over the summer, 44.8 % [95 % CI 43.9-45.7 %] over the winter, and 51.1 % [95 % CI 50.2-51.6 %] for the whole year. While total winter loss was one of the lowest reported in 8 years, 66 % of all beekeepers had higher losses than they deemed acceptable. C1 [Lee, Kathleen V.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Steinhauer, Nathalie; Rennich, Karen; vanEngelsdorp, Dennis] Univ Maryland, Dept Entomol, State Coll, MD 20742 USA. [Wilson, Michael E.; Skinner, John A.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Tarpy, David R.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Caron, Dewey M.; Sagili, Ramesh] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Rose, Robyn] USDA, Anim & Plant Hlth Inspect Serv, Riverdale, MD 20737 USA. [Delaplane, Keith S.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Baylis, Kathy] Univ Illinois, Dept Agr & Consumer Econ, Urbana, IL 61801 USA. [Lengerich, Eugene J.] Penn State Univ, Dept Publ Hlth Sci, Hershey, PA 17033 USA. [Wilkes, James T.] Appalachian State Univ, Dept Comp Sci, Boone, NC 28608 USA. [Pettis, Jeff] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. RP vanEngelsdorp, D (reprint author), Univ Maryland, Dept Entomol, Plant Sci Bldg, State Coll, MD 20742 USA. EM dennis.vanengelsdorp@gmail.com OI Lengerich, Eugene/0000-0001-9872-1647 FU Coordinated Agricultural Project (CAP) grant from US Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) FX We thank all respondents for their participation. We thank Brian Decker, Heather Eversole, Rachel Fahey, Andrew Garavito, and Meghan McConnell for entering the paper surveys into the database. Our gratitude goes out to the many beekeeping organizations, industry leaders, and beekeeping clubs that forwarded our appeal for participation emails. A special thank you is owed to USDA APHIS, the Apiary Inspectors of America, Eastern Apiculture Society, the American Honey Producers Association, the American Beekeeping Federation, Brushy Mountain Bee Farm, Bee Culture magazine, and American Bee Journal for sending out participation requests to their on-line audiences. This project was funded by a Coordinated Agricultural Project (CAP) grant from US Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA): the Bee Informed Partnership and includes, in addition to several of the authors, Marla Spivak, Angela Spleen, Jerry Hayes, Robert Snyder, Ben Sallman, Liana Teigen, Ellen Topitzhofer, Dan Wyns, Danielle Downey, Lauren Rusert, Ed Levi, Shayne Madella, Grace Kunkel, Marjorie Gurganus, Karen Roccasecca, and Robyn Underwood, and the USDA-ARS Areawide Program on Bee Health. Thank you to the reviewers for the helpful comments. NR 44 TC 36 Z9 38 U1 14 U2 70 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2015 VL 46 IS 3 BP 292 EP 305 DI 10.1007/s13592-015-0356-z PG 14 WC Entomology SC Entomology GA CH6GP UT WOS:000354134900004 ER PT J AU de Guzman, LI Rinderer, TE Frake, AM AF de Guzman, Lilia I. Rinderer, Thomas E. Frake, Amanda M. TI The effects of diet, mating duration, female to male ratios, and temperature on ovary activation, mating success, and fecundity of Aethina tumida SO APIDOLOGIE LA English DT Article DE Aethina tumida; fecundity; ovary activation; diet; mating duration; mating partner ID SMALL HIVE BEETLES; COLONIES APIS-MELLIFERA; HONEY-BEES; COLEOPTERA NITIDULIDAE; CAPENSIS ESCH.; OVIPOSITION; REMOVAL; POLLEN; BROOD; EGGS AB The effects of natural diet (brood, pollen, honey, and their various combinations), mating duration (1 day versus 2 days), female (F) to male (M) ratio (1F/1M, 2F/1M, and 1F/2M), and temperature (34 versus 27-29 A degrees C) on ovary activation, mating success, and fecundity of small hive beetles (SHBs) were studied. Our results indicated that regardless of mating duration, (1) a diet of brood, pollen, and honey presented together supported the highest fecundity; (2) intake of protein-rich diets encouraged ovary activation and egg-laying; and (3) diet of honey alone did not encourage ovary activation, mating success, and egg-laying at room temperature. Regardless of the number of males available for mating, egg-laying varied through time. Overall, females that were presented with two males for a 5-day mating period had higher fecundity than females provided with one male or three male partners. High temperature accelerated ovary activation and egg-laying regardless of female to male ratio during a 15-h mating period. Knowledge of these factors that influence fecundity helps elucidate why SHBs are very successful pests of honey bees. C1 [de Guzman, Lilia I.; Rinderer, Thomas E.; Frake, Amanda M.] ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. RP de Guzman, LI (reprint author), ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM Lilia.DeGuzman@ARS.USDA.GOV NR 29 TC 2 Z9 2 U1 1 U2 23 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2015 VL 46 IS 3 BP 326 EP 336 DI 10.1007/s13592-014-0325-y PG 11 WC Entomology SC Entomology GA CH6GP UT WOS:000354134900007 ER PT J AU Dolwick, P Akhtar, F Baker, KR Possiel, N Simon, H Tonnesen, G AF Dolwick, Pat Akhtar, Farhan Baker, Kirk R. Possiel, Norm Simon, Heather Tonnesen, Gail TI Comparison of background ozone estimates over the western United States based on two separate model methodologies SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Ozone; Background ozone; US background; Zero-out modeling; Source apportionment; CMAQ; CAMx ID SOURCE APPORTIONMENT; BOUNDARY-LAYER; NORTH-AMERICA; AIR; POLICY; VARIABILITY; EMISSIONS; TRANSPORT; EPISODES; US AB Two separate air quality model methodologies for estimating background ozone levels over the western U.S. are compared in this analysis. The first approach is a direct sensitivity modeling approach that considers the ozone levels that would remain after certain emissions are entirely removed (i.e., zero-out modeling). The second approach is based on an instrumented air quality model which tracks the formation of ozone within the simulation and assigns the source of that ozone to pre-identified categories (i.e., source apportionment modeling). This analysis focuses on a definition of background referred to as U.S. background (USB) which is designed to represent the influence of all sources other than U.S. anthropogenic emissions. Two separate modeling simulations were completed for an April October 2007 period, both focused on isolating the influence of sources other than domestic manmade emissions. The zero-out modeling was conducted with the Community Multiscale Air Quality (CMAQ) model and the source apportionment modeling was completed with the Comprehensive Air Quality Model with Extensions (CAMx). Our analysis shows that the zero-out and source apportionment techniques provide relatively similar estimates of the magnitude of seasonal mean daily 8-h maximum U.S. background ozone at locations in the western U.S. when base case model ozone biases are considered. The largest differences between the two sets of USB estimates occur in urban areas where interactions with local NOx emissions can be important, especially when ozone levels are relatively low. Both methodologies conclude that seasonal mean daily 8-h maximum U.S. background ozone levels can be as high as 40 -45 ppb over rural portions of the western U.S. Background fractions tend to decrease as modeled total ozone concentrations increase, with typical fractions of 75-100 percent on the lowest ozone days (<25 ppb) and typical fractions between 30 and 50% on days with ozone above 75 ppb. The finding that estimates of background ozone are not strongly dependent on the technique applied lends credibility to this and earlier modeling work. Published by Elsevier Ltd. C1 [Dolwick, Pat; Baker, Kirk R.; Possiel, Norm; Simon, Heather] US EPA, Res Triangle Pk, NC 27711 USA. [Akhtar, Farhan] USDA, Washington, DC 20520 USA. [Tonnesen, Gail] US EPA, Denver, CO 80202 USA. RP Dolwick, P (reprint author), US EPA, Res Triangle Pk, NC 27711 USA. EM dolwick.pat@epa.gov RI simon, heather/E-4392-2011 OI simon, heather/0000-0001-7254-3360 NR 42 TC 8 Z9 8 U1 2 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAY PY 2015 VL 109 BP 282 EP 296 DI 10.1016/j.atmosenv.2015.01.005 PG 15 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CH6MF UT WOS:000354150000031 ER PT J AU Burley, JD Theiss, S Bytnerowicz, A Gertler, A Schilling, S Zielinska, B AF Burley, Joel D. Theiss, Sandra Bytnerowicz, Andrzej Gertler, Alan Schilling, Susan Zielinska, Barbara TI Surface ozone in the Lake Tahoe Basin SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Lake Tahoe; Portable ozone monitor; Passive samplers; Spatial interpolation; Ozone exposure indices; HYSPLIT ID SAN-BERNARDINO MOUNTAINS; SIERRA-NEVADA; WESTERN SLOPE; CALIFORNIA; EXPOSURE; VEGETATION; OXIDATION; URBAN AB Surface ozone (O-3) concentrations were measured in and around the Lake Tahoe Basin using both active monitors (2010) and passive samplers (2002, 2010). The 2010 data from active monitors indicate average summertime diurnal maxima of approximately 50-55 ppb. Some site-to-site variability is observed within the Basin during the well-mixed hours of 10:00 to 17:00 PST, but large differences between different sites are observed in the late evening and pre-dawn hours. The observed trends correlate most strongly with elevation, topography, and surface vegetation. High elevation sites with steeply sloped topography and drier ground cover experience elevated O-3 concentrations throughout the night because they maintain good access to downward mixing of O-3-rich air from aloft with smaller losses due to dry deposition. Low elevation sites with flat topography and more dense surface vegetation experience low O-3 concentrations in the pre-dawn hours because of greatly reduced downward mixing coupled with enhanced O-3 removal via efficient dry deposition. Additionally, very high average O-3 concentrations were measured with passive samplers in the middle of the Lake in 2010. This latter result likely reflects diminished dry deposition to the surface of the Lake. High elevation Tahoe Basin sites with exposure to nocturnal O-3-rich air from aloft experience daily maxima of 8-h average O-3 concentrations that are frequently higher than concurrent maxima from the polluted upwind comparison sites of Sacramento, Folsom, and Placerville. Wind rose analyses of archived NAM 12 km meteorological data for the summer of 2010 suggest that some of the sampling sites situated near the shoreline may have experienced onshore "lake breezes" during daytime hours and/or off-shore "land breezes" during the night. Back-trajectory analysis with the HYSPLIT model suggests that much of the ozone measured at Lake Tahoe results from the transport of "polluted background" air into the Basin from upwind pollution source regions. Calculation of ozone exposure indices indicates that the two most polluted sites sampled by active monitors in 2010 the highest Genoa Peak site, located on the eastern side of the Lake at an elevation of 2734 m above sea level, and Angora Lookout, located to the south southwest (SSW) of the Lake at an elevation of 2218 m above sea level likely experienced some phytotoxic impacts, while the other Tahoe Basin locations received lower ozone exposures. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Burley, Joel D.] St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. [Theiss, Sandra; Gertler, Alan; Zielinska, Barbara] Desert Res Inst, Reno, NV 89512 USA. [Bytnerowicz, Andrzej; Schilling, Susan] US Forest Serv, USDA, Pacific Southwest Res Stn, Riverside, CA 92507 USA. RP Burley, JD (reprint author), St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. EM jburley@stmarys-ca.edu FU USDA Forest Service through the Tahoe Science Program - Southern Nevada Public Lands Management Act (SNPLMA) FX This study was supported in part by a grant from the USDA Forest Service through the Tahoe Science Program funded by the Southern Nevada Public Lands Management Act (SNPLMA). The authors wish to thank: Katy Orr for overseeing the data downloads and in-field calibrations of the portable ozone monitors during the summer of 2010; Joey Keely, Chris Engelhardt, Paul McCulloch and Shana Gross of the USDA Forest Service lake Tahoe Basin Management Unit for logistical help and data collection; Andrew Strain and Casey Blann of Heavenly Mountain Skiing Operations, Dennis Malone of Thunderbird Lodge, and Jack Coughlin of Diamond Peak Skiing Operations for assisting with the selection of the sampling sites; Brant C. Allen of the UC-Davis Environmental Research Center and Chris Ross of the USDA Forest Service PSW Research Station for help with the installations; and Jonathan Long and Tiffany Van Huysen of the USDA Forest Service PSW Research Station for administrative support. A special thanks is also extended to our scientific collaborators: Suraj Ahuja, Ricardo Cisneros, Glen Shaw, Don Schweizer, and Trent Procter of the US Forest Service Region 5; Leora Nanus of San Francisco State University; Mark McDaniel of Desert Research Institute; James Sickman and Michael Bell of University of California; and Miriam Rorig of USDA Forest Service PNW Research Station. Thanks also to Nyssa Perryman for her help and support. Finally, the authors gratefully acknowledge the NOAA Air Resources laboratory (ARL) for the provision of the HYSPLIT transport and dispersion model and/or READY website (http://www.ready.noaa.gov) used in this publication. NR 41 TC 4 Z9 5 U1 5 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD MAY PY 2015 VL 109 BP 351 EP 369 DI 10.1016/j.atmosenv.2015.02.001 PG 19 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CH6MF UT WOS:000354150000037 ER PT J AU Wallace, JB Eggert, SL Meyer, JL Webster, JR AF Wallace, J. Bruce Eggert, Susan L. Meyer, Judy L. Webster, Jackson R. TI Stream invertebrate productivity linked to forest subsidies: 37 stream-years of reference and experimental data SO ECOLOGY LA English DT Article DE allochthonous inputs; Coweeta Hydrologic Laboratory, North Carolina, USA; detritus; functional groups; invertebrate assemblages; organic matter quality; predator-prey relationships; riparian connectivity; secondary production; substratum relationships; whole-stream experiment; woody materials ID ORGANIC-MATTER; FOOD WEBS; HEADWATER STREAM; CHIRONOMIDAE DIPTERA; SECONDARY PRODUCTION; ECOSYSTEM FUNCTION; LEAF BREAKDOWN; MONTANE STREAM; WOOD BIOFILM; ENERGY-FLOW AB Riparian habitats provide detrital subsidies of varying quantities and qualities to recipient ecosystems. We used long-term data from three reference streams (covering 24 stream-years) and 13-year whole-stream organic matter manipulations to investigate the influence of terrestrial detrital quantity and quality on benthic invertebrate community structure, abundance, biomass, and secondary production in rockface (RF) and mixed substrates (MS) of forested headwater streams. Using a mesh canopy covering the entire treatment stream, we examined effects of litter exclusion, small-and large-wood removal, and addition of artificial wood (PVC) and leaves of varying quality on organic matter standing crops and invertebrate community structure and function. We assessed differences in functional feeding group distribution between substrate types as influenced by organic matter manipulations and long-term patterns of predator and prey production in manipulated vs. reference years. Particulate organic matter standing crops in MS of the treatment stream declined drastically with each successive year of litter exclusion, approaching zero after three years. Monthly invertebrate biomass and annual secondary production was positively related to benthic organic matter in the MS habitats. Rockface habitats exhibited fewer changes than MS habitats across all organic matter manipulations. With leaf addition, the patterns of functional group distribution among MS and RF habitats returned to patterns seen in reference streams. Secondary production per unit organic matter standing crop was greatest for the leaf addition period, followed by the reference streams, and significantly less for the litter exclusion and wood removal periods. These data indicate that the limited organic matter remaining in the stream following litter exclusion and wood removal was more refractory than that in the reference streams, whereas the added leaf material was more labile and readily converted into invertebrate production. Predator production and total production were tightly coupled in reference and treatment streams, indicating strong relationships between predators and their prey. Results from the artificial wood addition demonstrate that physical structure alone will not restore invertebrate productivity without detrital resources from the riparian forest. Our long-term studies conducted over three decades at the ecosystem scale unequivocally show the necessity of maintaining and restoring aquatic-terrestrial linkages in forested headwater streams. C1 [Wallace, J. Bruce; Eggert, Susan L.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Wallace, J. Bruce; Meyer, Judy L.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Eggert, Susan L.] USDA, Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Webster, Jackson R.] Virginia Polytech Inst & State Univ, Dept Biol, Blacksburg, VA 24061 USA. RP Wallace, JB (reprint author), Univ Georgia, Dept Entomol, Athens, GA 30602 USA. EM bwallace@uga.edu FU National Science Foundation [BSR83-16082, BSR87-18005, DEB-9207498, DEB-9629268, DEB-0212315] FX We thank G. J. Lugthart, T. F. Cuffney, A. D. Huryn, M. R. Whiles, F. Smith-Cuffney, A. Lingle, T. Pressley, G. Schurr, K. Chung, D. Imm, B. Goldowitz, D. Giles, B. Keeley, T. Siler, D. Hart, E. Fish, S. Entrekin, W. Cross, J. Davis, A. Braccia, and the many other graduate and undergraduate students who have assisted with the collection of the 37 stream-year invertebrate and organic matter data sets. We thank Sarah Wallace Petruziello for assistance with graphics and W. T. Swank and J. Vose for site support. Comments from William Sobczak and two anonymous reviewers greatly improved the manuscript. Funding from the National Science Foundation (Grants BSR83-16082, BSR87-18005, DEB-9207498, DEB-9629268, and DEB-0212315) made the collection of these long-term data possible. NR 72 TC 9 Z9 10 U1 10 U2 58 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD MAY PY 2015 VL 96 IS 5 BP 1213 EP 1228 DI 10.1890/14-1589.1 PG 16 WC Ecology SC Environmental Sciences & Ecology GA CH6BB UT WOS:000354119300007 PM 26236836 ER PT J AU Ip, HS Torchetti, MK Crespo, R Kohrs, P DeBruyn, P Mansfield, KG Baszler, T Badcoe, L Bodenstein, B Shearn-Bochsler, V Killian, ML Pedersen, JC Hines, N Gidlewski, T DeLiberto, T Sleeman, JM AF Ip, Hon S. Torchetti, Mia Kim Crespo, Rocio Kohrs, Paul DeBruyn, Paul Mansfield, Kristin G. Baszler, Timothy Badcoe, Lyndon Bodenstein, Barbara Shearn-Bochsler, Valerie Killian, Mary Lea Pedersen, Janice C. Hines, Nichole Gidlewski, Thomas DeLiberto, Thomas Sleeman, Jonathan M. TI Novel Eurasian Highly Pathogenic Avian Influenza A H5 Viruses in Wild Birds, Washington, USA, 2014 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID SOUTH-KOREA AB Novel Eurasian lineage avian influenza A(H5N8) virus has spread rapidly and globally since January 2014. In December 2014, H5N8 and reassortant H5N2 viruses were detected in wild birds in Washington, USA, and subsequently in backyard birds. When they infect commercial poultry, these highly pathogenic viruses pose substantial trade issues. C1 [Ip, Hon S.] US Geol Survey, Natl Wildlife Hlth Ctr, Wildlife Dis Diagnost Labs Branch, Diagnost Virol Lab, Madison, WI USA. [Bodenstein, Barbara; Shearn-Bochsler, Valerie; Sleeman, Jonathan M.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Torchetti, Mia Kim; Killian, Mary Lea; Pedersen, Janice C.; Hines, Nichole] USDA, Ames, IA USA. [Crespo, Rocio; Baszler, Timothy] Washington State Univ, Pullman, WA 99164 USA. [Kohrs, Paul; Baszler, Timothy; Badcoe, Lyndon] Washington Dept Agr, Olympia, WA USA. [DeBruyn, Paul; Mansfield, Kristin G.] Washington Dept Fish & Wildlife, Olympia, WA USA. [Gidlewski, Thomas; DeLiberto, Thomas] USDA, Ft Collins, CO USA. RP Torchetti, MK (reprint author), USDA APHIS VS Natl Serv Vet Labs, 1920 Dayton Ave, Ames, IA 50010 USA. EM Mia.Kim.Torchetti@aphis.usda.gov NR 12 TC 64 Z9 65 U1 3 U2 21 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAY PY 2015 VL 21 IS 5 BP 886 EP 890 DI 10.3201/eid2105.142020 PG 5 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA CG9BW UT WOS:000353610400025 PM 25898265 ER PT J AU Zhou, Y Mohan, A Moore, DC Lin, LJ Zhou, FL Cao, J Wu, Q Qin, YX Reginato, AM Ehrlich, MG Yang, WT AF Zhou, Yi Mohan, Aron Moore, Douglas C. Lin, Liangjun Zhou, Frank Li Cao, Jay Wu, Qian Qin, Yi-Xian Reginato, Anthony M. Ehrlich, Michael G. Yang, Wentian TI SHP2 regulates osteoclastogenesis by promoting preosteoclast fusion SO FASEB JOURNAL LA English DT Article DE M-CSF; RANKL; Nfatc1 ID COLONY-STIMULATING FACTOR; TYROSINE-PHOSPHATASE SHP-2; ACTIVATED T-CELLS; NOONAN-SYNDROME; SIGNALING PATHWAY; NUCLEAR FACTOR; RANK LIGAND; DC-STAMP; C-FOS; DIFFERENTIATION AB Genes that regulate osteoclast (OC) development and function in both physiologic and disease conditions remain incompletely understood. Shp2 (the Src homology-2 domain containing protein tyrosine phosphatase 2), a ubiquitously expressed cytoplasmic protein tyrosine phosphatase, is implicated in regulating M-CSF and receptor activator of nuclear factor-B ligand (RANKL)-evoked signaling; its role in osteoclastogenesis and bone homeostasis, however, remains unknown. Using a tissue-specific gene knockout approach, we inactivated Shp2 expression in murine OCs. Shp2 mutant mice are phenotypically osteopetrotic, featuring a marked increase of bone volume (BV)/total volume (TV) (+42.8%), trabeculae number (Tb.N) (+84.1%), structure model index (+119%), and a decrease of trabecular thickness (Tb.Th) (-34.1%) and trabecular spacing (Tb.Sp) (-41.0%). Biochemical analyses demonstrate that Shp2 is required for RANKL-induced formation of giant multinucleated OCs by up-regulating the expression of nuclear factor of activated T cells, cytoplasmic 1 (Nfatc1), a master transcription factor that is indispensable for terminal OC differentiation. Shp2 deletion, however, has minimal effect on M-CSF-dependent survival and proliferation of OC precursors. Instead, its deficiency aborts the fusion of OC precursors and formation of multinucleated OCs and decreases bone matrix resorption. Moreover, pharmacological intervention of Shp2 is sufficient to prevent preosteoclast fusion in vitro. These findings uncover a novel mechanism through which Shp2 regulates osteoclastogenesis by promoting preosteoclast fusion. Shp2 or its signaling partners could potentially serve as pharmacological targets to regulate the population of OCs locally and/or systematically, and thus treat OC-related diseases, such as periprosthetic osteolysis and osteoporosis. C1 [Zhou, Yi] Boston Childrens Hosp, Stem Cell Program, Boston, MA USA. [Zhou, Yi] Harvard Univ, Sch Med, Boston, MA USA. [Mohan, Aron; Moore, Douglas C.; Ehrlich, Michael G.; Yang, Wentian] Brown Univ, Dept Orthopaed, Alpert Med Sch, Providence, RI 02912 USA. [Mohan, Aron; Moore, Douglas C.; Ehrlich, Michael G.; Yang, Wentian] Rhode Isl Hosp, Providence, RI USA. [Lin, Liangjun; Qin, Yi-Xian] SUNY Stony Brook, Dept Biomed Engn, Stony Brook, NY 11794 USA. [Zhou, Frank Li] CALTECH, Engn & Appl Sci, Pasadena, CA 91125 USA. [Cao, Jay] USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Wu, Qian] Univ Connecticut, Ctr Hlth, Dept Pathol & Lab Med, Farmington, CT USA. [Reginato, Anthony M.] Brown Univ, Alpert Med Sch, Div Rheumatol, Providence, RI 02912 USA. [Reginato, Anthony M.] Rhode Isl Hosp, Providence, RI USA. RP Yang, WT (reprint author), 1 Hoppin St,Coro 402E, Providence, RI 02903 USA. EM wyang@lifespan.org FU U.S. National Institutes of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases [R21AR57156]; NIH National Institute of General Medical Sciences [P20 GM103468]; Pediatric Orthopaedic Society of North America; Orthopaedic Research and Education Foundation; U.S. Department of Agriculture Research Service [5450-51000-046-00D] FX The authors thank Dr. Nhiem Tran for critical reading, Ms. Xiaohong Wang for histological section and staining, and Dr. T. Taniguchi for pMX-IRES-GFP and pMX-Nfatc1/IRES-GFP constructs. The authors are grateful to Mr. Howard Yang (Moses Brown High School) for assistance in performing experiments and data collection. This work was supported in part by the U.S. National Institutes of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases Grant R21AR57156 (to W.Y.) and NIH National Institute of General Medical Sciences Grant P20 GM103468. This study was also aided by a grant from the Pediatric Orthopaedic Society of North America and the Orthopaedic Research and Education Foundation (to W.Y.), and the U.S. Department of Agriculture Research Service program Grant #5450-51000-046-00D (to J.C.). NR 42 TC 4 Z9 4 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD MAY PY 2015 VL 29 IS 5 BP 1635 EP 1645 DI 10.1096/fj.14-260844 PG 11 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA CH5ZJ UT WOS:000354114600003 PM 25593124 ER PT J AU Peterson, BC Peatman, E Ourth, DD Waldbieser, GC AF Peterson, Brian C. Peatman, E. Ourth, D. D. Waldbieser, G. C. TI Effects of a phytogenic feed additive on growth performance, susceptibility of channel catfish to Edwardsiella ictaluri and levels of mannose binding lectin SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Channel catfish; Essential oils; Mannose binding lectin; Innate immune response ID C-TYPE LECTIN; REAL-TIME PCR; ADAPTIVE IMMUNITY; PUNCTATUS; INNATE; SURVIVAL; STRESS; SPLEEN; GENES AB A study was conducted to investigate the effect of a phytogenic feed additive (Digestarom (R) P.E.P. MGE; containing the essential oils carvacrol, thymol, anethol, and limonene) on growth performance and disease susceptibility to Edwardsiella ictaluri. Two hundred and fifty juvenile channel catfish, Ictalurus punctatus (7.2 +/- 0.1 g) were allotted into the following treatments: Control (floating diet) and EO (floating diet supplemented with essential oils). The fish were fed their respective diets for 6 weeks. At the end of the study, all fish were exposed to virulent E. ictaluri by bath immersion (1.9 x 10(7) cfu/mL; final concentration). Plasma and tissue samples were taken to quantify protein and mRNA expression levels of mannose binding lectin (MBL). Weight gain and food conversion ratio were similar between treatments. After exposing fish to virulent E. ictaluri and monitoring mortality for 21 days, survival was 43% higher (69.5 vs 48.4%) in fish fed EO compared to fish not treated with EO (P < 0.05). One day after challenge, plasma MBL levels were down-regulated in the non-treated fish compared to non-challenged fish. In the EO fish, MBL levels were similar to non-challenged fish but significantly higher than non-treated fed fish (P < 0.001). By d 7, plasma MBL levels increased in non-treated fed fish to levels observed in the EO and non-challenged fish. On d 14, MBL mRNA levels were upregulated 15-fold in fish fed EO compared to non-treated fed fish and non-challenged fish (P < 0.001). The results demonstrate that essential oils improved survival of channel catfish challenged with E. ictaluri. Mechanisms through which essential oils improve survival may involve MBL. Published by Elsevier Ltd. C1 [Peterson, Brian C.; Waldbieser, G. C.] USDA ARS, Thad Cochran Natl Warmwater Aquaculture Ctr, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. [Peatman, E.] Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA. [Peatman, E.] Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA. [Ourth, D. D.] Univ Memphis, Dept Biol Sci, Memphis, TN 38152 USA. RP Peterson, BC (reprint author), USDA ARS, Thad Cochran Natl Warmwater Aquaculture Ctr, Warmwater Aquaculture Res Unit, POB 38, Stoneville, MS 38776 USA. EM brian.peterson@ars.usda.gov NR 31 TC 3 Z9 3 U1 1 U2 13 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD MAY PY 2015 VL 44 IS 1 BP 21 EP 25 DI 10.1016/j.fsi.2015.01.027 PG 5 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA CH0WU UT WOS:000353743900003 PM 25659231 ER PT J AU Qiao, G Xu, DH Wang, ZS Jang, IK Qi, ZT Zhang, MM Kim, SK AF Qiao, Guo Xu, De-Hai Wang, Zishen Jang, In-Kwon Qi, Zhitao Zhang, Mingming Kim, Su-Kyoung TI Comparison of immune response of Pacific white shrimp, Litopenaeus vannamei, after multiple and single infections with WSSV and Vibrio anguillarum SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Litopenaeus vannamei; Immune response; Multiple infections; White spot syndrome virus (WSSV); Vibrio anguillarum ID SPOT SYNDROME VIRUS; SERINE PROTEINASE HOMOLOG; PROPHENOLOXIDASE-ACTIVATING SYSTEM; PATTERN-RECOGNITION PROTEIN; REAL-TIME PCR; PENAEUS-MONODON; MOLECULAR CHARACTERIZATION; INNATE IMMUNITY; HEMOCYTIC PHAGOCYTOSIS; EXPRESSION AB Our previous study demonstrated that Pacific white shrimp (Litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen Clang et al., 2014). For better understanding the defense mechanism against white spot syndrome virus (WSSV) and Vibrio anguillarum, immune responses of shrimp were evaluated in this study. The mRNA expression levels of five immune-related genes were analyzed by quantitative reverse real-time PCR, which included proPO-activating enzyme 1 (PPAE1), PPAE2, proPO activating factor (PPAF), masquerade-like serine proteinase (Mas) and ras-related nuclear gene (Ran). Results demonstrated that the transcription was suppressed more intensively in the multiple infection group than those in single infection groups. The transcriptional suppression was directly related to the higher mortality. The hypoimmunity could benefit pathogen invasion, replication and release of toxin in vivo. Results in this study will help to understand immune defense mechanism after shrimp were infected by multiple pathogens in aquaculture. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Qiao, Guo; Wang, Zishen; Qi, Zhitao; Zhang, Mingming] Yancheng Inst Technol, Dept Ocean Technol, Coll Chem & Biol Engn, Yancheng 224051, Peoples R China. [Xu, De-Hai] Agr Res Serv, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. [Jang, In-Kwon; Kim, Su-Kyoung] West Sea Fisheries Res Inst, Natl Fisheries Res & Dev Inst, Inchon 400420, South Korea. RP Zhang, MM (reprint author), Yancheng Inst Technol, Dept Ocean Technol, Coll Chem & Biol Engn, Yancheng 224051, Peoples R China. EM zhangmingm44ok@163.com; isagemang@naver.com FU National Fisheries Research and Development Institute (NFRDI), Republic of Korea [kjc 2014018, RP-2015-AQ-007] FX This work was supported by the Project "Talent introduction program at Yancheng Institute of Technology" (kjc 2014018), "Extension of biofloc technology for marine shrimp farming" (RP-2015-AQ-007), National Fisheries Research and Development Institute (NFRDI), Republic of Korea. NR 51 TC 0 Z9 0 U1 2 U2 14 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD MAY PY 2015 VL 44 IS 1 BP 257 EP 264 DI 10.1016/j.fsi.2015.02.009 PG 8 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA CH0WU UT WOS:000353743900030 PM 25700782 ER PT J AU Annous, BA Burke, A AF Annous, Bassam A. Burke, Angela TI Development of Combined Dry Heat and Chlorine Dioxide Gas Treatment with Mechanical Mixing for Inactivation of Salmonella enterica Serovar Montevideo on Mung Bean Seeds SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; ALFALFA SEEDS; SPROUTS; DISINFECTION; EFFICACY; PRODUCE; SAFETY; WATER AB Foodborne outbreaks have been associated with the consumption of fresh sprouted beans. The sprouting conditions of mung bean seeds provide optimal conditions of temperature and relative humidity for any potential pathogenic contaminant on the seeds to grow. The lack of a kill step postsprouting is a major safety concern. Thus, the use of a kill step on the seeds prior to a sprouting step would enhance the safety of fresh sprouts. The objective of this work was to evaluate the effectiveness of the combined thermal and chlorine dioxide gas (3.5 mg/liter of air) treatment with mechanical mixing (tumbling) to eliminate Salmonella on artificially inoculated Mung bean seeds. Although no viable Salmonella was recovered from seeds treated in hot water at 60 degrees C for 2 h, these treated seeds failed to germinate. Dry heat treatments (55, 60, or 70 degrees C) for up to 8 h reduced Salmonella populations in excess of 3 log CFU/g. The use of tumbling, while treating the seeds, resulted in up to 1.6 log CFU/g reduction in Salmonella populations compared with no tumbling. Dry heat treatment at 65 degrees C for 18 h with tumbling resulted in a complete inactivation of Salmonella populations on inoculated seeds with low inoculum levels (2.83 log CFU/g) as compared with high inoculum levels (4.75 log CFU/g). The increased reductions in pathogenic populations on the seeds with the use of tumbling could be attributed to increased uniformity of heat transfer and exposure to chlorine dioxide gas. All treated seeds were capable of germinating, as well as the nontreated controls. These results suggest that this combined treatment would be a viable process for enhancing the safety of fresh sprouts. C1 [Annous, Bassam A.; Burke, Angela] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Annous, BA (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM bassam.ann.ous@ars.usda.gov NR 16 TC 0 Z9 0 U1 1 U2 13 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2015 VL 78 IS 5 BP 868 EP 872 DI 10.4315/0362-028X.JFP-14-422 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CH6KR UT WOS:000354146000001 PM 25951379 ER PT J AU Paoli, GC Wijey, C Uhlich, GA AF Paoli, George C. Wijey, Chandi Uhlich, Gaylen A. TI Genetically Marked Strains of Shiga Toxin-Producing O157:H7 and Non-O157 Escherichia coli: Tools for Detection and Modeling SO JOURNAL OF FOOD PROTECTION LA English DT Article ID HEMOLYTIC-UREMIC SYNDROME; POLYMERASE-CHAIN-REACTION; HEMORRHAGIC COLITIS; UNITED-STATES; GROUND-BEEF; VIRULENCE FACTORS; SUICIDE VECTOR; SEROGROUPS O26; GENE-CLUSTER; SEROTYPE AB Shiga toxin producing E. coli (STEC) is an important group of foodborne pathogens in the United States and worldwide. Nearly half of STEC-induced diarrheal disease in the United States is caused by serotype O157:H7, while non-O157 STEC account for the remaining illnesses. Thus, the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service has instituted regulatory testing of beef products and has a zero-tolerance policy for regulatory samples that test positive for STEC O157:H7 and six other non-O157 STEC (serogroups O26, O45, O103, O111, O121, and O145). In this study, positive control (PC) strains for the detection of STEC O157:H7 and the six USDA-regulated non-O157 STEC were constructed. To ensure that the food testing samples are not cross-contaminated by the PC sample, it is important that the STEC-PC strains are distinguishable from STEC isolated from test samples. The PC strains were constructed by integrating a unique DNA target sequence and a gene for spectinomycin (Sp) resistance into the chromosomes of the seven STEC strains. End-point and real-time PCR assays were developed for the specific detection of the PC strains and were tested using 93 strains of E. coli (38 STEC O157:H7, at least 6 strains of each of the USDA-regulated non-O157 STEC, and 2 commensal E. coli) and 51 strains of other bacteria (30 species from 20 genera). The PCR assays demonstrated high specificity for the unique target sequence. The target sequence was detectable by PCR after 10 culture passages (similar to 100 generations), demonstrating the stability of the integrated target sequence. In addition, the strains were tested for their potential use in modeling the growth of STEC. Plating the PC strains mixed with ground beef flora on modified rainbow agar containing Sp eliminated the growth of the background flora that grew on modified rainbow agar without Sp. Thus, these strains could be used to enumerate and model the growth of STEC in the presence of foodborne background flora. C1 [Paoli, George C.; Wijey, Chandi; Uhlich, Gaylen A.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Paoli, GC (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM George.Paoli@ars.usda.gov NR 39 TC 2 Z9 3 U1 0 U2 11 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2015 VL 78 IS 5 BP 888 EP 901 DI 10.4315/0362-028X.JFP-14-472 PG 14 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CH6KR UT WOS:000354146000004 PM 25951382 ER PT J AU Porto-Fett, ACS Campano, SG Shoyer, BA Israeli, D Oser, A Luchansky, JB AF Porto-Fett, Anna C. S. Campano, Stephen G. Shoyer, Bradley A. Israeli, David Oser, Alan Luchansky, John B. TI Comparative Efficacy of Potassium Levulinate with and without Potassium Diacetate and Potassium Propionate versus Potassium Lactate and Sodium Diacetate for Control of Listeria monocytogenes on Commercially Prepared Uncured Turkey Breast SO JOURNAL OF FOOD PROTECTION LA English DT Article ID VACUUM-SEALED PACKAGES; TO-EAT FOODS; ORGANIC-ACIDS; BEEF BOLOGNA; LAURIC ARGINATE; SALMONELLA SPP.; RISK-ASSESSMENT; UNITED-STATES; PORK BOLOGNA; FRANKFURTERS AB We evaluated the efficacy of potassium levulinate (KLEV; 0.0, 1.0, 1.5, and 2.0%) with and without a blend of potassium propionate (0.1%) and potassium diacetate (0.1%) (KPD) versus a blend of potassium lactate (1.8%) and sodium diacetate (0.125%) (KLD) for inhibiting Listeria monocytogenes on commercially prepared, uncured turkey breast during refrigerated storage. Product formulated with KLD or KLEV (1.5%) was also subsequently surface treated with 44 ppm of a solution of lauric arginate (LAE). Slices (ca. 1.25 cm thick and 100 g) of turkey breast formulated with or without antimicrobials were surface inoculated on both the top and bottom faces to a target level of ca. 3.5 log CFU per slice with a five-strain cocktail of L. monocyto genes, vacuum sealed, and then stored at 4 degrees C for up to 90 days. Without inclusion of antimicrobials in the formulation, pathogen levels increased by ca. 5.2 log CFU per slice, whereas with the inclusion of 1.0 to 2.0% KLEV pathogen levels increased by only ca. 2.9 to 0.8 log CFU per slice after 90 days at 4 degrees C. When 1.0% KLEV and KPD were included as ingredients, pathogen levels increased by ca. 0.8 log CFU per slice after storage at 4 degrees C for 90 days, whereas a decrease of ca. 0.7 log CFU per slice was observed when 1.5 or 2.0% KLEV and KPD were included as ingredients. When used alone, KPD was not effective (>= 5.8-log increase). As expected, KLD was effective at suppressing L. monocytogenes in uncured turkey breast. When uncured turkey breast was formulated with KLD or KLEV (1.5%) or without antimicrobials and subsequently surface treated with LAB, pathogen levels decreased by ca. 1.0 log CFU per package within 2 h; no differences (P >= 0.01) were observed in pathogen levels for product surface treated with or without LAB and stored for 90 days. Our results validate the use of KLEV to inhibit outgrowth of L. monocyto genes during refrigerated storage of uncured turkey breast. KLEV is at least as effective as KLD as an antilisterial agent. C1 [Porto-Fett, Anna C. S.; Shoyer, Bradley A.; Luchansky, John B.] ARS, USDA, Wyndmoor, PA 19038 USA. [Campano, Stephen G.] Hawkins Inc, Roseville, MN 55413 USA. [Israeli, David] Mrs Resslers Food Prod, Philadelphia, PA 19120 USA. [Oser, Alan] Food Safety Connect, Blacksville, WV 26521 USA. RP Porto-Fett, ACS (reprint author), ARS, USDA, Wyndmoor, PA 19038 USA. EM anna.portofett@ars.usda.gov NR 44 TC 1 Z9 1 U1 4 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2015 VL 78 IS 5 BP 927 EP 933 DI 10.4315/0362-028X.JFP-14-467 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CH6KR UT WOS:000354146000008 PM 25951386 ER PT J AU Fan, XT Felker, P Sokorai, KJ AF Fan, Xuetong Felker, Peter Sokorai, Kimberly J. TI Decontamination of Mesquite Pod Flour Naturally Contaminated with Bacillus cereus and Formation of Furan by Ionizing Irradiation SO JOURNAL OF FOOD PROTECTION LA English DT Article ID VOLATILE SULFUR-COMPOUNDS; ELECTRON-BEAM IRRADIATION; OFF-ODOR VOLATILES; SALMONELLA-TYPHIMURIUM; GAMMA-IRRADIATION; LIPID OXIDATION; PROCESSED FOODS; RAW RICE; PORK; RADIATION AB Mesquite pod flour produced from nitrogen-fixing trees of the Prosopis species has a unique aroma and flavor that is preferred by some consumers. Due to the presence of wildlife, grazing domestic animals, and insects, the pods have a high potential of being contaminated with human pathogenic bacteria, such as Bacillus cereus. Nonthermal processing technologies are helpful to reduce the population of microorganisms in the flour because heating deteriorates the characteristic flavor. A study was conducted to investigate the efficacy of ionizing radiation in decontaminating two types of mesquite pod flours (Prosopis alba and Prosopis pallida) naturally contaminated with B. cereus and the effects of irradiation on the formation of furan, a possible human carcinogen. Results showed that the populations of B. cereus were 3.8 and 5.4 log CFU/g in nonirradiated P. alba and P. pallida flours, respectively, and populations of microflora, mesophilic spores, B. cereus, and B. cereus spores decreased with increasing radiation doses. At 6 kGy, the populations fell below 1 log CFU/g. Irradiation at 6 kGy had no significant effect on the fructose, glucose, or sucrose content of the flour. Nonirradiated P. alba and P. pallida flours contained 13.0 and 3.1 ng/g of furan, respectively. Furan levels increased with irradiation doses at rates of 2.3 and 2.4 ng/g/kGy in the two flours. The level of 3-methylbutanal was reduced or not affected by irradiation, while the hexanal level was increased. Our results suggested that irradiation was effective in decontaminating contaminated mesquite flour. The significance of furan formation and possible changes in flavor due to irradiation may need to be further examined. C1 [Fan, Xuetong; Sokorai, Kimberly J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Felker, Peter] Casa de Mesquite LLC, Hollister, CA 95023 USA. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov NR 45 TC 0 Z9 0 U1 2 U2 12 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2015 VL 78 IS 5 BP 954 EP 962 DI 10.4315/0362-028X.JFP-14-572 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CH6KR UT WOS:000354146000012 PM 25951390 ER PT J AU Swartz, RS Luchansky, JB Kulas, M Shoyer, BA Shane, LE Strasser, H Munson, M Porto-Fett, ACS AF Swartz, Richard S. Luchansky, John B. Kulas, Megan Shoyer, Bradley A. Shane, Laura E. Strasser, Hannah Munson, Madison Porto-Fett, Anna C. S. TI Thermal Inactivation of Shiga Toxin-Producing Escherichia coli Cells within Cubed Beef Steaks following Cooking on a Griddle SO JOURNAL OF FOOD PROTECTION LA English DT Article ID O157H7; O157/H7; APPLIANCES AB Thermal inactivation of Shiga toxin producing Escherichia coli (STEC) cells within knitted/cubed beef steaks following cooking on a nonstick griddle was quantified. Both faces of each beef cutlet (ca. 64 g; ca. 8.5 cm length by 10.5 cm width by 0.75 cm height) were surface inoculated (ca. 6.6 log CFU/g) with 250 mu l of a rifampin-resistant cocktail composed of single strains from each of eight target serogroups of STEC: O26:H11, O45:H2, O103:H2,O104:H4, O111:H-, O121:H19, O145:NM, and O157:H7. Next, inoculated steaks were (i) passed once through a mechanical tenderizer and then passed one additional time through the tenderizer perpendicular to the orientation of the first pass (single cubed steak; SCS) or (ii) passed once through a mechanical tenderizer, and then two tenderized cutlets were knitted together by passage concomitantly through the tenderizer two additional times perpendicular to the orientation of the previous pass (double cubed steak; DCS). SCS and DCS were individually cooked for up to 3.5 min per side in 30 ml of extra virgin olive oil heated to 191.5 degrees C (376.7 degrees F) on a hard-anodized aluminum nonstick griddle using a flat-surface electric ceramic hot plate. Regardless of steak preparation (i.e., single versus double cubed steaks), as expected, the longer the cooking time, the higher the final internal temperature, and the greater the inactivation of STEC cells within cubed steaks. The average final internal temperatures of SCS cooked for up 2.5 min and DCS cooked for up to 3.5 min ranged from 59.8 to 94.7 degrees C and 40.3 to 82.2 degrees C, respectively. Cooking SCS and DCS on an aluminum griddle set at ca. 191.5 degrees C for 0.5 to 2.5 min and 1.0 to 3.5 min per side, respectively, resulted in total reductions in pathogen levels of ca. 1.0 to >= 6.8 log CFU/g. These data validated that cooking SCS (ca. 0.6 cm thick) or DCS (ca. 1.3 cm thick) on a nonstick aluminum griddle heated at 191.5 degrees C for at least 1.25 and 3.0 min per side, respectively, was sufficient to achieve a 5.0-log reduction in the levels of the single strains from each of the eight target STEC serogroups tested. C1 [Swartz, Richard S.] Penn State Univ, University Pk, PA 16802 USA. [Luchansky, John B.; Shoyer, Bradley A.; Shane, Laura E.; Porto-Fett, Anna C. S.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Kulas, Megan] Kansas State Univ, Manhattan, KS 66506 USA. [Strasser, Hannah] Delaware Valley Coll, Doylestown, PA 18901 USA. [Munson, Madison] Drexel Univ, Philadelphia, PA 19014 USA. RP Porto-Fett, ACS (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM anna.portofett@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2012-68003-30155] FX We extend our sincere appreciation to John Phillips, Nelly Osoria, and Ashley Hallowell (USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA) for their assistance on this project. This project was also supported, in part, by Agriculture and Food Research Initiative Competitive Grant 2012-68003-30155 from the USDA National Institute of Food and Agriculture. NR 22 TC 2 Z9 2 U1 2 U2 8 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2015 VL 78 IS 5 BP 1013 EP 1017 DI 10.4315/0362-028X.JFP-14-454 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CH6KR UT WOS:000354146000021 PM 25951399 ER PT J AU Kinzli, KD Gensler, D DeJonge, K Oad, R Shafike, N AF Kinzli, Kristoph-Dietrich Gensler, David DeJonge, Kendall Oad, Ramchand Shafike, Nabil TI Validation of a Decision Support System for Improving Irrigation System Performance SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE Decision support systems; DSS validation; Soil moisture modeling; Efficient water management; Irrigation scheduling; Model validation ID MODEL; SOIL; EVAPORATION; SIMULATION; WATER AB To address water shortage and improve water delivery operations, decision support systems (DSSs) have been developed and utilized throughout the United States and the world. One critical aspect that is often neglected during the development and implementation of DSSs is validation, which can result in flawed water distribution and rejection of the DSS by water users and managers. This paper presents the results of a significant validation effort for a DSS in the Middle Rio Grande Conservancy District (MRGCD). The validation resulted in a refined application efficiency of 45%, a refined readily available water for farmers to irrigate to a value of 20%, and a Nash-Sutcliffe modeling efficiency of 0.86 for soil moisture depletion patterns. Overall, the validation and refinement of input parameters resulted in a DSS model that accurately predicts evaportranspiration and can be used to schedule water delivery. The refinement of the DSS input parameters resulted in an increased 15,600 acre-ft diversion suggested by the DSS, indicating that the original DSS input parameters would have adversely affected farmers in the MRGCD. The paper showed that validation of a DSS is crucial if such a program is to be successfully utilized to deliver irrigation water. C1 [Kinzli, Kristoph-Dietrich] Florida Gulf Coast Univ, Dept Environm & Civil Engn, Ft Myers, FL 33965 USA. [Gensler, David] Middle Rio Grande Conservancy Dist, Albuquerque, NM 87102 USA. [DeJonge, Kendall] ARS, USDA, Ft Collins, CO 80526 USA. [Oad, Ramchand] Colorado State Univ, Dept Civil Engn, Ft Collins, CO 80523 USA. [Shafike, Nabil] New Mexico Interstate Stream Commiss, Albuquerque, NM 87109 USA. RP Kinzli, KD (reprint author), Florida Gulf Coast Univ, Dept Environm & Civil Engn, Ft Myers, FL 33965 USA. EM kkinzli@fgcu.edu; dgensler@mrgcd.com; Kendall.DeJonge@ars.usda.gov; oad@engr.colostate.edu; nabil.shafike@state.nm.us FU New Mexico Interstate Stream Commission; Endangered Species Act Collaborative Program FX The writers would like to thank the staff of the Middle Rio Grande Conservancy District, especially Matt Martinez for assistance in installing the EC-20 probes and Rick Altenberg for assistance with geographic information system (GIS) and mapping. The writers would also like to thank the New Mexico Interstate Stream Commission and the Endangered Species Act Collaborative Program for the funding to undertake the research reported in this paper. The advice and assistance of Gaylon Cambell, Doug Cobos, and T. J. Clevenger of Decagon Devices are also recognized. The writers would also like to thank Troy Bauder of Colorado State University and Stephen Smith for reviewing a previous version of this paper. NR 26 TC 2 Z9 2 U1 1 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD MAY PY 2015 VL 141 IS 5 DI 10.1061/(ASCE)IR.1943-4774.0000829 PG 10 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA CH5TL UT WOS:000354098100009 ER PT J AU Rammer, DR Zelinka, SL AF Rammer, Douglas R. Zelinka, Samuel L. TI Withdrawal Strength and Bending Yield Strength of Stainless Steel Nails SO JOURNAL OF STRUCTURAL ENGINEERING LA English DT Article DE Wood; Joints; Design; Stainless steel; Nails; Bending; Stainless steel; Nail withdrawal; National design specifications for wood construction; Nail bending yield strength; Wood structures ID COMMON WIRE NAILS; WOOD AB It has been well established that stainless steel nails have superior corrosion performance compared to carbon steel or galvanized nails in treated wood; however, their mechanical fastening behavior is unknown. In this paper, the performance of stainless steel nails is examined with respect to two important properties used in wood connection design: withdrawal strength and nail bending yield strength. Different nail diameters, wood specific gravities, and nail manufacturers were examined. The current withdrawal design equations, developed from carbon steel nail data, overpredict the expected withdrawal strength when used for stainless steel nails, reducing the safety factor. As a result, a new equation was developed to predict the nail stainless steel withdrawal capacity. The data further indicate that nail bending yield strength values for stainless steel were similar to carbon steel data. C1 [Rammer, Douglas R.; Zelinka, Samuel L.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Rammer, DR (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM drammer@fs.fed.us; szelinka@fs.fed.us NR 18 TC 0 Z9 0 U1 0 U2 2 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9445 EI 1943-541X J9 J STRUCT ENG JI J. Struct. Eng. PD MAY PY 2015 VL 141 IS 5 AR 04014134 DI 10.1061/(ASCE)ST.1943-541X.0001088 PG 7 WC Construction & Building Technology; Engineering, Civil SC Construction & Building Technology; Engineering GA CH5RC UT WOS:000354092000012 ER PT J AU Bisantino, T Bingner, R Chouaib, W Gentile, F Liuzzi, GT AF Bisantino, T. Bingner, R. Chouaib, W. Gentile, F. Liuzzi, G. Trisorio TI ESTIMATION OF RUNOFF, PEAK DISCHARGE AND SEDIMENT LOAD AT THE EVENT SCALE IN A MEDIUM-SIZE MEDITERRANEAN WATERSHED USING THE ANNAGNPS MODEL SO LAND DEGRADATION & DEVELOPMENT LA English DT Article DE soil erosion; suspended sediment transport; modelling; continuous monitoring ID DIGITAL ELEVATION MODELS; AGRICULTURAL WATERSHEDS; BARE SOIL; TRANSPORT; EROSION; BASIN; FLOW; VALIDATION; RAINFALL; AGNPS AB In this paper, the Annualized Agricultural Non-Point Source (AnnAGNPS) model has been used to estimate runoff, peak discharge and sediment load at the event scale in a Mediterranean watershed. The study area is the Carapelle torrent, Southern Italy (area=506km(2)), where continuous rainfall, streamflow and sediment load data are available. Nineteen flood events have been registered in the period 2007-2009 and were used for the application of the model. The aim of the paper is to evaluate the predictive accuracy of the model at the event scale, in a medium-size watershed, given the specific conditions of the semi-arid environments. A sensitivity analysis has been carried out to assign the correct parameterization: the mean normalized output variation of the most meaningful input parameters pointed out the influence of the curve number on runoff, peak discharge and sediment load predictions (values greater than 1); the MN Manning's roughness coefficient and K, C and P factors of the universal soil loss equation showed a moderate influence on sediment load simulations (values between 05 and 1). The selection of the Soil Conservation Service synthetic storm types has been based on the observed storm events analysis to improve the peak discharge simulations. The model prediction has proved to be good for runoff (R-2=074, NSE=075, W=092) and peak discharge (R-2=085, NSE=070, W=094), and satisfactory for sediment yield (R-2=070, NSE=063, W=091). The relative error is lower for high events; this result is quite interesting in semi-arid environments, where most of the annual sediment yield is concentrated in a few, severe events. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Bisantino, T.; Gentile, F.; Liuzzi, G. Trisorio] Univ Bari, Dept Agr & Environm Sci, I-70126 Bari, Italy. [Bingner, R.] ARS, USDA, Natl Sedimentat Lab, Watershed Phys Proc Res Unit, Oxford, MS USA. [Bingner, R.] ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS USA. [Chouaib, W.] Natl Res Inst Rural Engn Water & Forestry, Tunis 2080, Tunisia. RP Bisantino, T (reprint author), Univ Bari, Dept Agr & Environm Sci, Via Amendola 165-A, I-70126 Bari, Italy. EM t.bisantino@gmail.com RI feng, yongzhong/F-5090-2012; OI feng, yongzhong/0000-0002-5202-4368; Gentile, Francesco/0000-0003-4462-0466 NR 59 TC 19 Z9 20 U1 7 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1085-3278 EI 1099-145X J9 LAND DEGRAD DEV JI Land Degrad. Dev. PD MAY PY 2015 VL 26 IS 4 BP 340 EP 355 DI 10.1002/ldr.2213 PG 16 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA CH4HY UT WOS:000353995800004 ER PT J AU Edelaar, P Roques, S Hobson, EA Da Silva, AG Avery, ML Russello, MA Senar, JC Wright, TF Carrete, M Tella, JL AF Edelaar, Pim Roques, Severine Hobson, Elizabeth A. Da Silva, Anders Goncalves Avery, Michael L. Russello, Michael A. Senar, Juan C. Wright, Timothy F. Carrete, Martina Tella, Jose L. TI Shared genetic diversity across the global invasive range of the monk parakeet suggests a common restricted geographic origin and the possibility of convergent selection SO MOLECULAR ECOLOGY LA English DT Article DE bottleneck; founder effect; invasion genetics; native origin; population genetic structure; selection ID MITOCHONDRIAL CONTROL REGION; MULTIPLE INTRODUCTIONS; POPULATION-STRUCTURE; BIOLOGICAL INVASIONS; MYIOPSITTA-MONACHUS; LIFE-HISTORY; PROGRAM; CONSEQUENCES; DISPERSAL; EVOLUTION AB While genetic diversity is hypothesized to be an important factor explaining invasion success, there is no consensus yet on how variation in source populations or demographic processes affects invasiveness. We used mitochondrial DNA haplotypic and microsatellite genotypic data to investigate levels of genetic variation and reconstruct the history of replicate invasions on three continents in a globally invasive bird, the monk parakeet (Myiopsitta monachus). We evaluated whether genetic diversity at invasive sites could be explained by (i) the native source populations from which they were derived and (ii) demographic bottlenecks during introduction. Genetic data indicated a localized source area for most sampled invasive populations, with limited evidence for admixing of native source populations. This pattern largely coincides with historical data on pet trade exports. However, the invasive populations are genetically more similar than predicted from the export data alone. The extent of bottleneck effects varied among invasive populations. The observed low genetic diversity, evidence of demographic contraction and restricted source area do not support the hypothesis that invasion is favoured by the mixing and recombining of genetic variation from multiple source populations. Instead, they suggest that reduced genetic variation through random processes may not inhibit successful establishment and invasion in this species. However, convergent selection across invasive sites could also explain the observed patterns of reduction and similarity in genetic variation and/or the restricted source area. In general, the alternative explanation of intraspecific variation in invasive potential among genotypes or geographic areas is neglected, but warrants more attention as it could inform comparative studies and management of biological invaders. C1 [Edelaar, Pim; Carrete, Martina] Univ Pablo de Olavide, Dept Mol Biol & Biochem Engn, ES-41013 Seville, Spain. [Edelaar, Pim; Roques, Severine; Carrete, Martina; Tella, Jose L.] CSIC, Estn Biol Donana, Dept Conservat Biol, Seville 41092, Spain. [Hobson, Elizabeth A.; Wright, Timothy F.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Hobson, Elizabeth A.] Univ Tennessee, Natl Inst Biol & Math Synth, Knoxville, TN 37996 USA. [Da Silva, Anders Goncalves] Monash Univ, Sch Biol Sci, Clayton, Vic, Australia. [Avery, Michael L.] USDA ARS, Wildlife Serv, Natl Wildlife Res Ctr, Gainesville, FL 32641 USA. [Russello, Michael A.] Univ British Columbia, Dept Biol, Kelowna, BC V1V 1V7, Canada. [Senar, Juan C.] Nat Hist Museum Barcelona, Barcelona 08003, Spain. RP Edelaar, P (reprint author), Univ Pablo de Olavide, Dept Mol Biol & Biochem Engn, ES-41013 Seville, Spain. EM edelaar@upo.es RI Tella, Jose/I-3707-2015; Carrete, Martina/A-6376-2009; Edelaar, Pim/P-5650-2014; CSIC, EBD Donana/C-4157-2011; OI Tella, Jose/0000-0002-3038-7424; Carrete, Martina/0000-0002-0491-2950; Edelaar, Pim/0000-0003-4649-6366; CSIC, EBD Donana/0000-0003-4318-6602; Goncalves da Silva, Anders/0000-0002-2257-8781 FU Fundacion Repsol; Spanish Ministry of Economy and Competitiveness [RYC-2011-07889, CGL2012-35232, CGL2012-38262]; Spanish Ministry of Economy and Competitiveness (ERDF); Canadian Foundation for Innovation; National Institutes of Health [S06GM008136]; National Institute for Mathematical and Biological Synthesis through NSF [DBI-1300426]; National Institute for Mathematical and Biological Synthesis (University of Tennessee, Knoxville); European COST Action 'ParrotNet' [ES1304]; [P08-RNM-4014] FX We thank Kevin Burgio, Miguel Santillan, the Laboratorio de Ecologia Molecular and the Estacion Biologica de Donana for providing help and samples; Jessica Eberhard, landowners in Argentina, Paraguay and Spain, James Lindsay (Florida Power and Light), Eric Tillman and Monte Chandler (USDA), Ana Anechina (Ayuntamento de Zaragoza), R. Riera and G. Blanco for helping us to obtain samples; and Enrique Bucher and Rosana Aramburu for information and discussion. Sampling protocols in Argentina were approved by the New Mexico State University Institutional Animal Care and Use Committee (protocol no. 2006-027), and permits for sampling in Entre Rios, La Pampa and Buenos Aires provinces were provided by the Argentinean Department of Natural Resources. Funding for the project was provided by P08-RNM-4014 and Fundacion Repsol (to JLT and MC), the Spanish Ministry of Economy and Competitiveness (RYC-2011-07889 and CGL2012-35232 with support from the ERDF to PE, and CGL2012-38262 to JCS), Canadian Foundation for Innovation (MR), National Institutes of Health (S06GM008136 to TFW) and the National Institute for Mathematical and Biological Synthesis (Postdoctoral Fellowship to EAH, through NSF #DBI-1300426 with additional support from the University of Tennessee, Knoxville). Barcelona data collection was partially supported by Servei de Vigilancia i Control de Plagues Urbanes from Agencia de Salut Publica de Barcelona. Further support was provided by the European COST Action 'ParrotNet' (ES1304). NR 56 TC 5 Z9 5 U1 0 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD MAY PY 2015 VL 24 IS 9 SI SI BP 2164 EP 2176 DI 10.1111/mec.13157 PG 13 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CH3JY UT WOS:000353928200018 PM 25873354 ER PT J AU Burrell, AM Pepper, AE Hodnett, G Goolsby, JA Overholt, WA Racelis, AE Diaz, R Klein, PE AF Burrell, A. Millie Pepper, Alan E. Hodnett, George Goolsby, John A. Overholt, William A. Racelis, Alexis E. Diaz, Rodrigo Klein, Patricia E. TI Exploring origins, invasion history and genetic diversity of Imperata cylindrica (L.) P. Beauv. (Cogongrass) in the United States using genotyping by sequencing SO MOLECULAR ECOLOGY LA English DT Review DE clonal; comparative genomics; invasive species; Poaceae; rhizome; SNP molecular markers ID GENUS SORGHUM POACEAE; BIOLOGICAL-CONTROL; PLANT INVASIONS; SEXUAL REPRODUCTION; POPULATION-GENETICS; HELIANTHUS-ANNUUS; CLONAL DIVERSITY; GENOME EVOLUTION; CANDIDATE GENES; GRASSES POACEAE AB Imperata cylindrica (Cogongrass, Speargrass) is a diploid C4 grass that is a noxious weed in 73 countries and constitutes a significant threat to global biodiversity and sustainable agriculture. We used a cost-effective genotyping-by-sequencing (GBS) approach to identify the reproductive system, genetic diversity and geographic origins of invasions in the south-eastern United States. In this work, we demonstrated the advantage of employing the closely related, fully sequenced crop species Sorghum bicolor (L.) Moench as a proxy reference genome to identify a set of 2320 informative single nucleotide and insertion-deletion polymorphisms. Genetic analyses identified four clonal lineages of cogongrass and one clonal lineage of Imperata brasiliensis Trin. in the United States. Each lineage was highly homogeneous, and we found no evidence of hybridization among the different lineages, despite geographical overlap. We found evidence that at least three of these lineages showed clonal reproduction prior to introduction to the United States. These results indicate that cogongrass has limited evolutionary potential to adapt to novel environments and further suggest that upon arrival to its invaded range, this species did not require local adaptation through hybridization/introgression or selection of favourable alleles from a broad genetic base. Thus, cogongrass presents a clear case of broad invasive success, across a diversity of environments, in a clonal organism with limited genetic diversity. C1 [Burrell, A. Millie; Klein, Patricia E.] Texas A&M Univ, Dept Hort Sci, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA. [Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Hodnett, George] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Goolsby, John A.] USDA ARS, Cattle Fever Tick Res Lab, Moore Air Base, Edinburg, TX 78541 USA. [Overholt, William A.; Diaz, Rodrigo] Univ Florida, Biol Control & Containment Lab, Ft Pierce, FL 34945 USA. [Racelis, Alexis E.] Univ Texas Pan Amer, Dept Biol, Edinburg, TX 78539 USA. RP Burrell, AM (reprint author), Texas A&M Univ, Dept Hort Sci, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA. EM amburrell@tamu.edu FU USDA-NIFA [2012-67013-19340]; Texas A&M AgriLife Research Genomics Seed Grant FX The authors wish to acknowledge the extraordinary generosity in sample collection by members of the Alabama Forestry Commission, Clemson Department of Plant Industries, the Florida Forest Service, the Georgia Forestry Commission, the Louisiana Forestry Commission, the Mississippi Forestry Commission and the Texas A&M Forest Service. We thank Jim Walley, Wallace Allred, Leland Cseke and Daniel Tarin for helpful discussions and sample material. We thank Natalie Patterson for help with DNA extraction and Illumina template library preparation. The authors also thank L.J. Grauke (USDA-ARS, College Station) and R. Daniel Lineberger for help with arc-GIS figure preparation. We would also like to thank the three anonymous reviewers for their valuable comments and suggestions. This work was supported by USDA-NIFA grant #2012-67013-19340 and by a Texas A&M AgriLife Research Genomics Seed Grant. NR 139 TC 4 Z9 4 U1 17 U2 94 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD MAY PY 2015 VL 24 IS 9 SI SI BP 2177 EP 2193 DI 10.1111/mec.13167 PG 17 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CH3JY UT WOS:000353928200019 PM 25864837 ER PT J AU Cornelis, MC Byrne, EM Esko, T Nalls, MA Ganna, A Paynter, N Monda, KL Amin, N Fischer, K Renstrom, F Ngwa, JS Huikari, V Cavadino, A Nolte, IM Teumer, A Yu, K Marques-Vidal, P Rawal, R Manichaikul, A Wojczynski, MK Vink, JM Zhao, JH Burlutsky, G Lahti, J Mikkila, V Lemaitre, RN Eriksson, J Musani, SK Tanaka, T Geller, F Luan, J Hui, J Magi, R Dimitriou, M Garcia, ME Ho, WK Wright, MJ Rose, LM Magnusson, PKE Pedersen, NL Couper, D Oostra, BA Hofman, A Ikram, MA Tiemeier, HW Uitterlinden, AG van Rooij, FJA Barroso, I Johansson, I Xue, L Kaakinen, M Milani, L Power, C Snieder, H Stolk, RP Baumeister, SE Biffar, R Gu, F Bastardot, F Kutalik, Z Jacobs, DR Forouhi, NG Mihailov, E Lind, L Lindgren, C Michaelsson, K Morris, A Jensen, M Khaw, KT Luben, RN Wang, JJ Mannisto, S Perala, MM Kahonen, M Lehtimaki, T Viikari, J Mozaffarian, D Mukamal, K Psaty, BM Doring, A Heath, AC Montgomery, GW Dahmen, N Carithers, T Tucker, KL Ferrucci, L Boyd, HA Melbye, M Treur, JL Mellstrom, D Hottenga, JJ Prokopenko, I Tonjes, A Deloukas, P Kanoni, S Lorentzon, M Houston, DK Liu, Y Danesh, J Rasheed, A Mason, MA Zonderman, AB Franke, L Kristal, BS Karjalainen, J Reed, DR Westra, HJ Evans, MK Saleheen, D Harris, TB Dedoussis, G Curhan, G Stumvoll, M Beilby, J Pasquale, LR Feenstra, B Bandinelli, S Ordovas, JM Chan, AT Peters, U Ohlsson, C Gieger, C Martin, NG Waldenberger, M Siscovick, DS Raitakari, O Eriksson, JG Mitchell, P Hunter, DJ Kraft, P Rimm, EB Boomsma, DI Borecki, IB Loos, RJF Wareham, NJ Vollenweider, P Caporaso, N Grabe, HJ Neuhouser, ML Wolffenbuttel, BHR Hu, FB Hypponen, E Jarvelin, MR Cupples, LA Franks, PW Ridker, PM van Duijn, CM Heiss, G Metspalu, A North, KE Ingelsson, E Nettleton, JA van Dam, RM Chasman, DI AF Cornelis, M. C. Byrne, E. M. Esko, T. Nalls, M. A. Ganna, A. Paynter, N. Monda, K. L. Amin, N. Fischer, K. Renstrom, F. Ngwa, J. S. Huikari, V. Cavadino, A. Nolte, I. M. Teumer, A. Yu, K. Marques-Vidal, P. Rawal, R. Manichaikul, A. Wojczynski, M. K. Vink, J. M. Zhao, J. H. Burlutsky, G. Lahti, J. Mikkila, V. Lemaitre, R. N. Eriksson, J. Musani, S. K. Tanaka, T. Geller, F. Luan, J. Hui, J. Maegi, R. Dimitriou, M. Garcia, M. E. Ho, W-K Wright, M. J. Rose, L. M. Magnusson, P. K. E. Pedersen, N. L. Couper, D. Oostra, B. A. Hofman, A. Ikram, M. A. Tiemeier, H. W. Uitterlinden, A. G. van Rooij, F. J. A. Barroso, I. Johansson, I. Xue, L. Kaakinen, M. Milani, L. Power, C. Snieder, H. Stolk, R. P. Baumeister, S. E. Biffar, R. Gu, F. Bastardot, F. Kutalik, Z. Jacobs, D. R., Jr. Forouhi, N. G. Mihailov, E. Lind, L. Lindgren, C. Michaelsson, K. Morris, A. Jensen, M. Khaw, K-T Luben, R. N. Wang, J. J. Mannisto, S. Perala, M-M Kahonen, M. Lehtimaki, T. Viikari, J. Mozaffarian, D. Mukamal, K. Psaty, B. M. Doering, A. Heath, A. C. Montgomery, G. W. Dahmen, N. Carithers, T. Tucker, K. L. Ferrucci, L. Boyd, H. A. Melbye, M. Treur, J. L. Mellstrom, D. Hottenga, J. J. Prokopenko, I. Toenjes, A. Deloukas, P. Kanoni, S. Lorentzon, M. Houston, D. K. Liu, Y. Danesh, J. Rasheed, A. Mason, M. A. Zonderman, A. B. Franke, L. Kristal, B. S. Karjalainen, J. Reed, D. R. Westra, H-J Evans, M. K. Saleheen, D. Harris, T. B. Dedoussis, G. Curhan, G. Stumvoll, M. Beilby, J. Pasquale, L. R. Feenstra, B. Bandinelli, S. Ordovas, J. M. Chan, A. T. Peters, U. Ohlsson, C. Gieger, C. Martin, N. G. Waldenberger, M. Siscovick, D. S. Raitakari, O. Eriksson, J. G. Mitchell, P. Hunter, D. J. Kraft, P. Rimm, E. B. Boomsma, D. I. Borecki, I. B. Loos, R. J. F. Wareham, N. J. Vollenweider, P. Caporaso, N. Grabe, H. J. Neuhouser, M. L. Wolffenbuttel, B. H. R. Hu, F. B. Hyppoenen, E. Jarvelin, M-R Cupples, L. A. Franks, P. W. Ridker, P. M. van Duijn, C. M. Heiss, G. Metspalu, A. North, K. E. Ingelsson, E. Nettleton, J. A. van Dam, R. M. Chasman, D. I. CA Coffee & Caffeine Genetics Consort Int Parkinson's Dis Genomics Conso North Amer Brain Expression Consor UK Brain Expression Consortium TI Genome-wide meta-analysis identifies six novel loci associated with habitual coffee consumption SO MOLECULAR PSYCHIATRY LA English DT Article ID CAFFEINE; PROTEIN; VARIANTS; RECEPTOR; BRAIN; GLUCOKINASE; INVOLVEMENT; BINDING; BDNF AB Coffee, a major dietary source of caffeine, is among the most widely consumed beverages in the world and has received considerable attention regarding health risks and benefits. We conducted a genome-wide (GW) meta-analysis of predominately regular-type coffee consumption (cups per day) among up to 91 462 coffee consumers of European ancestry with top single-nucleotide polymorphisms (SNPs) followed-up in similar to 30 062 and 7964 coffee consumers of European and African-American ancestry, respectively. Studies from both stages were combined in a trans-ethnic meta-analysis. Confirmed loci were examined for putative functional and biological relevance. Eight loci, including six novel loci, met GW significance (log(10)Bayes factor (BF) > 5.64) with per-allele effect sizes of 0.03-0.14 cups per day. Six are located in or near genes potentially involved in pharmacokinetics (ABCG2, AHR, POR and CYP1A2) and pharmacodynamics (BDNF and SLC6A4) of caffeine. Two map to GCKR and MLXIPL genes related to metabolic traits but lacking known roles in coffee consumption. Enhancer and promoter histone marks populate the regions of many confirmed loci and several potential regulatory SNPs are highly correlated with the lead SNP of each. SNP alleles near GCKR, MLXIPL, BDNF and CYP1A2 that were associated with higher coffee consumption have previously been associated with smoking initiation, higher adiposity and fasting insulin and glucose but lower blood pressure and favorable lipid, inflammatory and liver enzyme profiles (P < 5x10(-8)). Our genetic findings among European and African-American adults reinforce the role of caffeine in mediating habitual coffee consumption and may point to molecular mechanisms underlying inter-individual variability in pharmacological and health effects of coffee. C1 [Cornelis, M. C.; Mozaffarian, D.; Curhan, G.; Pasquale, L. R.; Chan, A. T.; Hunter, D. J.; Rimm, E. B.; Hu, F. B.] Harvard Univ, Sch Med, Brigham & Womens Hosp, Channing Div Network Med, Boston, MA 02215 USA. [Cornelis, M. C.; Jensen, M.; Mozaffarian, D.; Rimm, E. B.; Hu, F. B.; Franks, P. W.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02215 USA. [Byrne, E. M.] Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia. [Esko, T.; Fischer, K.; Maegi, R.; Milani, L.; Mihailov, E.; Metspalu, A.] Univ Tartu, Estonian Genome Ctr, EE-50090 Tartu, Estonia. [Esko, T.] Childrens Hosp Boston, Div Endocrinol, Boston, MA USA. [Esko, T.] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02215 USA. 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[Mannisto, S.; Perala, M-M] Natl Inst Hlth & Welf, Dept Chron Dis Prevent, Helsinki, Finland. [Kahonen, M.] Tampere Univ Hosp, Dept Clin Physiol, Tampere, Finland. [Kahonen, M.; Lehtimaki, T.] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland. [Lehtimaki, T.] Univ Tampere, Fimlab Labs, Dept Clin Chem, FIN-33101 Tampere, Finland. [Viikari, J.] Univ Turku, Dept Med, Turku, Finland. [Viikari, J.] Turku Univ Hosp, FIN-20520 Turku, Finland. [Mozaffarian, D.; Rimm, E. B.; Hu, F. B.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Mozaffarian, D.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Mukamal, K.] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA. [Psaty, B. M.; Siscovick, D. S.] Univ Washington, Dept Epidemiol, Cardiovasc Hlth Res Unit, Seattle, WA 98195 USA. [Psaty, B. M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98195 USA. [Psaty, B. M.] Grp Hlth Cooperat Puget Sound, Grp Hlth Res Inst, Seattle, WA USA. [Doering, A.] Helmholtz Zentrum Munchen, Inst Epidemiol, Munich, Germany. [Heath, A. C.] Washington Univ, Dept Psychiat, St Louis, MO USA. [Dahmen, N.] Johannes Gutenberg Univ Mainz, Dept Psychiat, Mainz, Germany. [Carithers, T.] Univ Mississippi, Sch Appl Sci, Oxford, MS USA. [Tucker, K. L.] Univ MA Lowell, Clin Lab & Nutr Sci, Lowell, MA USA. [Prokopenko, I.] Univ London Imperial Coll Sci Technol & Med, Dept Genom Common Dis, London, England. [Toenjes, A.; Stumvoll, M.] Univ Leipzig, Dept Med, D-04109 Leipzig, Germany. [Toenjes, A.; Stumvoll, M.] Univ Leipzig, IFB Adipos Dis, D-04109 Leipzig, Germany. [Deloukas, P.; Kanoni, S.] Queen Mary Univ London, William Harvey Res Inst, Barts & London Sch Med & Dent, London, England. [Deloukas, P.] King Abdulaziz Univ, Jeddah 21413, Saudi Arabia. [Houston, D. K.] Wake Forest Sch Med, Winston Salem, NC USA. [Houston, D. K.; Liu, Y.] Wake Forest Univ Hlth Sci, Winston Salem, NC USA. [Rasheed, A.; Saleheen, D.] Ctr Noncommunicable Dis, Karachi, Pakistan. [Mason, M. A.; Evans, M. K.] NIA, Hlth Dispar Res Sect, Clin Res Branch, NIH, Baltimore, MD 21224 USA. [Zonderman, A. B.] NIA, Lab Personal & Cognit, NIH, Baltimore, MD 21224 USA. [Franke, L.; Karjalainen, J.; Westra, H-J] Univ Groningen, Univ Med Ctr Groningen, Dept Genet, Groningen, Netherlands. [Kristal, B. S.] Brigham & Womens Hosp, Dept Neurosurg, Boston, MA 02115 USA. [Kristal, B. S.] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA. [Reed, D. R.] Monell Chem Senses Ctr, Philadelphia, PA 19104 USA. [Pasquale, L. R.] Massachusetts Eye & Ear Infirm, Boston, MA 02114 USA. [Bandinelli, S.] Azienda Sanitaria Firenze, Geriatr Unit, Florence, Italy. [Ordovas, J. M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chan, A. T.] MA Gen Hosp, Div Gastroenterol, Boston, MA USA. [Peters, U.; Neuhouser, M. L.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98104 USA. [Waldenberger, M.] Helmholtz Zentrum Munchen, Res Unit Mol Epidemiol, Munich, Germany. [Raitakari, O.] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, FIN-20520 Turku, Finland. [Eriksson, J. G.] Univ Helsinki, Dept Gen Practice & Primary Hlth Care, Helsinki, Finland. [Eriksson, J. G.] Univ Helsinki, Cent Hosp, Unit Gen Practice, Helsinki, Finland. [Hunter, D. J.; Kraft, P.] Harvard Univ, Sch Publ Hlth, Program Genet Epidemiol & Stat Genet, Boston, MA 02115 USA. [Loos, R. J. F.] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10029 USA. [Loos, R. J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10029 USA. [Grabe, H. J.] Univ Med Greifswald, HELIOS Hosp Stralsund, Dept Psychiat & Psychotherapy, Greifswald, Germany. [Wolffenbuttel, B. H. R.] Univ Groningen, Dept Endocrinol, Univ Med Ctr Groningen, Groningen, Netherlands. [Hyppoenen, E.] Univ S Australia, Sch Populat Hlth, Adelaide, SA 5001, Australia. [Hyppoenen, E.] South Australian Hlth & Med Res Inst, Adelaide, SA, Australia. [Jarvelin, M-R] Natl Inst Hlth & Welf, Dept Children & Young People & Families, Oulu, Finland. [Jarvelin, M-R] Oulu Univ Hosp, Unit Primary Care, Oulu, Finland. [Cupples, L. A.] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Franks, P. W.] Umea Univ, Med Sect, Dept Publ Hlth & Clin Med, Umea, Sweden. [van Duijn, C. M.] Netherlands Consortium Hlth Ageing, Leiden, Netherlands. [van Duijn, C. M.] Natl Genom Initiat, Leiden, Netherlands. [Nettleton, J. A.] Univ Texas Hlth Sci Ctr Houston, Div Epidemiol Human Genet & Environm Sci, Houston, TX 77030 USA. [van Dam, R. M.] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore 117548, Singapore. [van Dam, R. M.] Natl Univ Singapore, Dept Med, Yong Loo Lin Sch Med, Singapore 117548, Singapore. [van Dam, R. M.] Natl Univ Hlth Syst, Singapore, Singapore. RP Cornelis, MC (reprint author), Harvard Univ, Sch Publ Hlth, Dept Nutr, 401 Pk Dr, Boston, MA 02215 USA. EM mcorneli@hsph.harvard.edu; dchasman@research.bwh.harvard.edu RI Colaus, PsyColaus/K-6607-2013; Waldenberger, Melanie/B-5355-2014; Prokopenko, Inga/H-3241-2014; Wright, Margaret/A-4560-2016; Hypponen, Elina/B-2596-2014; Deloukas, Panos/B-2922-2013; Franke, Lude/P-7036-2016; Karjalainen, Juha/P-8624-2016; Magnusson, Patrik/C-4458-2017 OI Jarvelin, Marjo-Riitta/0000-0002-2149-0630; Eriksson, Johan/0000-0002-2516-2060; Zonderman, Alan B/0000-0002-6523-4778; corvol, jean-christophe/0000-0001-7325-0199; Tiemeier, Henning/0000-0002-4395-1397; Plagnol, Vincent/0000-0002-5597-9215; Ikram, Mohammad Arfan/0000-0003-0372-8585; Esko, Tonu/0000-0003-1982-6569; Gieger, Christian/0000-0001-6986-9554; van Dam, Rob/0000-0002-7354-8734; Mannisto, Satu/0000-0002-8668-3046; Mellstrom, Dan/0000-0003-2761-3723; Lahti, Jari/0000-0002-4310-5297; Luben, Robert/0000-0002-5088-6343; Wolffenbuttel, Bruce H.R./0000-0001-9262-6921; Forouhi, Nita/0000-0002-5041-248X; Waldenberger, Melanie/0000-0003-0583-5093; Kaakinen, Marika/0000-0002-9228-0462; HO, WEANG KEE/0000-0002-8269-7344; Magi, Reedik/0000-0002-2964-6011; Prokopenko, Inga/0000-0003-1624-7457; Wright, Margaret/0000-0001-7133-4970; Hypponen, Elina/0000-0003-3670-9399; Deloukas, Panos/0000-0001-9251-070X; Franke, Lude/0000-0002-5159-8802; NR 43 TC 18 Z9 19 U1 6 U2 46 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1359-4184 EI 1476-5578 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD MAY PY 2015 VL 20 IS 5 BP 647 EP 656 DI 10.1038/mp.2014.107 PG 10 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA CH0JS UT WOS:000353706400016 ER PT J AU Li, FG Fan, GY Lu, CR Xiao, GH Zou, CS Kohel, RJ Ma, ZY Shang, HH Ma, XF Wu, JY Liang, XM Huang, G Percy, RG Liu, K Yang, WH Chen, WB Du, XM Shi, CC Yuan, YL Ye, WW Liu, X Zhang, XY Liu, WQ Wei, HL Wei, SJ Huang, GD Zhang, XL Zhu, SJ Zhang, H Sun, FM Wang, XF Liang, J Wang, JH He, Q Huang, LH Wang, J Cui, JJ Song, GL Wang, KB Xu, X Yu, JZ Zhu, YX Yu, SX AF Li, Fuguang Fan, Guangyi Lu, Cairui Xiao, Guanghui Zou, Changsong Kohel, Russell J. Ma, Zhiying Shang, Haihong Ma, Xiongfeng Wu, Jianyong Liang, Xinming Huang, Gai Percy, Richard G. Liu, Kun Yang, Weihua Chen, Wenbin Du, Xiongming Shi, Chengcheng Yuan, Youlu Ye, Wuwei Liu, Xin Zhang, Xueyan Liu, Weiqing Wei, Hengling Wei, Shoujun Huang, Guodong Zhang, Xianlong Zhu, Shuijin Zhang, He Sun, Fengming Wang, Xingfen Liang, Jie Wang, Jiahao He, Qiang Huang, Leihuan Wang, Jun Cui, Jinjie Song, Guoli Wang, Kunbo Xu, Xun Yu, John Z. Zhu, Yuxian Yu, Shuxun TI Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution SO NATURE BIOTECHNOLOGY LA English DT Article ID TRANSPOSABLE ELEMENTS; ARABIDOPSIS-THALIANA; CELLULOSE SYNTHESIS; MAXIMUM-LIKELIHOOD; CELL ELONGATION; PLANT GENOMES; FIBER; BIOSYNTHESIS; TRANSCRIPTS; PREDICTION AB Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (A(t)D(t)) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%similar to 96.7% of the A(t)D(t) genome, are anchored and oriented to 26 pseudochromosomes. Comparison of this Gossypium hirsutum A(t)D(t) genome with the already sequenced diploid Gossypium arboreum (AA) and Gossypium raimondii (DD) genomes revealed conserved gene order. Repeated sequences account for 67.2% of the AtDt genome, and transposable elements (TEs) originating from D-t seem more active than from A(t). Reduction in the A(t)D(t) genome size occurred after allopolyploidization. The A or A(t) genome may have undergone positive selection for fiber traits. Concerted evolution of different regulatory mechanisms for Cellulose synthase (CesA) and 1-Aminocyclopropane-1-carboxylic acid oxidase1 and 3 (ACO1,3) may be important for enhanced fiber production in Gossypium hirsutum. C1 [Li, Fuguang; Lu, Cairui; Zou, Changsong; Shang, Haihong; Ma, Xiongfeng; Wu, Jianyong; Liu, Kun; Yang, Weihua; Du, Xiongming; Yuan, Youlu; Ye, Wuwei; Zhang, Xueyan; Wei, Hengling; Wei, Shoujun; Cui, Jinjie; Song, Guoli; Wang, Kunbo; Yu, Shuxun] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China. [Fan, Guangyi; Liang, Xinming; Chen, Wenbin; Shi, Chengcheng; Liu, Xin; Liu, Weiqing; Huang, Guodong; Zhang, He; Sun, Fengming; Liang, Jie; Wang, Jiahao; He, Qiang; Huang, Leihuan; Wang, Jun; Xu, Xun] BGI Shenzhen, Shenzhen, Peoples R China. [Xiao, Guanghui; Huang, Gai; Zhu, Yuxian] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China. [Xiao, Guanghui; Huang, Gai; Zhu, Yuxian] Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China. [Xiao, Guanghui; Huang, Gai; Zhu, Yuxian] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China. [Kohel, Russell J.; Percy, Richard G.] USDA ARS, Crop Germplasm Res Unit, Southern Plains Agr Res Ctr, College Stn, TX USA. [Ma, Zhiying; Wang, Xingfen] Agr Univ Hebei, Key Lab Crop Germplasm Resources Hebei, Baoding, Peoples R China. [Zhang, Xianlong] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China. [Zhu, Shuijin] Zhejiang Univ, Dept Agron, Hangzhou 310003, Zhejiang, Peoples R China. RP Zhu, YX (reprint author), Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China. EM John.Yu@ars.usda.gov; zhuyx@whu.edu.cn; Yu@cricaas.com.cn RI Wang, Jun/C-8434-2016; Wang, Jun/B-9503-2016 OI Wang, Jun/0000-0002-8540-8931; Wang, Jun/0000-0002-2113-5874 FU Major Program of Joint Funds (Sinkiang) of the National Natural Science Foundation of China [U1303282]; National Natural Science Foundation of China [90717009]; 863 National High-Tech Research Development Program in China [2013AA102601]; United States Department of Agriculture, Agricultural Research Service (USDA-ARS project) [3091-21000-038-01] FX We are grateful to Q. Li for his assistance with data analysis and with the early phase of manuscript preparation. This work was supported by the Major Program of Joint Funds (Sinkiang) of the National Natural Science Foundation of China (grant U1303282), the National Natural Science Foundation of China (grant 90717009) and the 863 National High-Tech Research Development Program in China (grant 2013AA102601), and United States Department of Agriculture, Agricultural Research Service (USDA-ARS project 3091-21000-038-01). NR 59 TC 111 Z9 144 U1 33 U2 115 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1087-0156 EI 1546-1696 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD MAY PY 2015 VL 33 IS 5 BP 524 EP U242 DI 10.1038/nbt.3208 PG 9 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CH8VP UT WOS:000354314500034 PM 25893780 ER PT J AU Zhang, TZ Hu, Y Jiang, WK Fang, L Guan, XY Chen, JD Zhang, JB Saski, CA Scheffler, BE Stelly, DM Hulse-Kemp, AM Wan, Q Liu, BL Liu, CX Wang, S Pan, MQ Wang, YK Wang, DW Ye, WX Chang, LJ Zhang, WP Song, QX Kirkbride, RC Chen, XY Dennis, E Llewellyn, DJ Peterson, DG Thaxton, P Jones, DC Wang, Q Xu, XY Zhang, H Wu, HT Zhou, L Mei, GF Chen, SQ Tian, Y Xiang, D Li, XH Ding, J Zuo, QY Tao, LN Liu, YC Li, J Lin, Y Hui, YY Cao, ZS Cai, CP Zhu, XF Jiang, Z Zhou, BL Guo, WZ Li, RQ Chen, ZJ AF Zhang, Tianzhen Hu, Yan Jiang, Wenkai Fang, Lei Guan, Xueying Chen, Jiedan Zhang, Jinbo Saski, Christopher A. Scheffler, Brian E. Stelly, David M. Hulse-Kemp, Amanda M. Wan, Qun Liu, Bingliang Liu, Chunxiao Wang, Sen Pan, Mengqiao Wang, Yangkun Wang, Dawei Ye, Wenxue Chang, Lijing Zhang, Wenpan Song, Qingxin Kirkbride, Ryan C. Chen, Xiaoya Dennis, Elizabeth Llewellyn, Danny J. Peterson, Daniel G. Thaxton, Peggy Jones, Don C. Wang, Qiong Xu, Xiaoyang Zhang, Hua Wu, Huaitong Zhou, Lei Mei, Gaofu Chen, Shuqi Tian, Yue Xiang, Dan Li, Xinghe Ding, Jian Zuo, Qiyang Tao, Linna Liu, Yunchao Li, Ji Lin, Yu Hui, Yuanyuan Cao, Zhisheng Cai, Caiping Zhu, Xiefei Jiang, Zhi Zhou, Baoliang Guo, Wangzhen Li, Ruiqiang Chen, Z. Jeffrey TI Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement SO NATURE BIOTECHNOLOGY LA English DT Article ID GENOME SEQUENCE; GENE-EXPRESSION; RNA-SEQ; BRASSICA-NAPUS; CELL-WALL; EVOLUTION; DATABASE; TOOL; ARABIDOPSIS; POLYPLOIDY AB Upland cotton is a model for polyploid crop domestication and transgenic improvement. Here we sequenced the allotetraploid Gossypium hirsutum L. acc. TM-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end sequences and genotype-by-sequencing genetic maps. We assembled and annotated 32,032 A-subgenome genes and 34,402 D-subgenome genes. Structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the A subgenome than in the D subgenome, suggesting asymmetric evolution. However, no genome-wide expression dominance was found between the subgenomes. Genomic signatures of selection and domestication are associated with positively selected genes (PSGs) for fiber improvement in the A subgenome and for stress tolerance in the D subgenome. This draft genome sequence provides a resource for engineering superior cotton lines. C1 [Zhang, Tianzhen; Hu, Yan; Fang, Lei; Guan, Xueying; Chen, Jiedan; Wan, Qun; Liu, Bingliang; Liu, Chunxiao; Wang, Sen; Pan, Mengqiao; Wang, Yangkun; Ye, Wenxue; Chang, Lijing; Zhang, Wenpan; Wang, Qiong; Xu, Xiaoyang; Zhang, Hua; Wu, Huaitong; Zhou, Lei; Mei, Gaofu; Chen, Shuqi; Tian, Yue; Xiang, Dan; Li, Xinghe; Ding, Jian; Cai, Caiping; Zhu, Xiefei; Zhou, Baoliang; Guo, Wangzhen; Chen, Z. Jeffrey] Nanjing Agr Univ, Cotton Hybrid R&D Engn Ctr, Minist Educ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China. [Jiang, Wenkai; Zhang, Jinbo; Wang, Dawei; Zuo, Qiyang; Tao, Linna; Liu, Yunchao; Li, Ji; Lin, Yu; Hui, Yuanyuan; Cao, Zhisheng; Jiang, Zhi; Li, Ruiqiang] Novogene Bioinformat Inst, Beijing, Peoples R China. [Fang, Lei; Song, Qingxin; Kirkbride, Ryan C.; Chen, Z. Jeffrey] Univ Texas Austin, Dept Mol Biosci, Ctr Computat Biol & Bioinformat, Austin, TX 78712 USA. [Fang, Lei; Guan, Xueying; Song, Qingxin; Kirkbride, Ryan C.; Chen, Z. Jeffrey] Univ Texas Austin, Dept Mol Biosci, Inst Cellular & Mol Biol, Austin, TX 78712 USA. [Saski, Christopher A.] Clemson Univ, Genom Inst, Clemson, SC USA. [Scheffler, Brian E.] ARS, USDA, MSA, Genom Lab, Stoneville, MS USA. [Stelly, David M.; Hulse-Kemp, Amanda M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Chen, Xiaoya] Chinese Acad Sci, Natl Key Lab Plant Mol Genet, Natl Plant Gene Res Ctr, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China. [Dennis, Elizabeth; Llewellyn, Danny J.] Commonwealth Sci & Ind Res Org, Plant Ind, Black Mt, Australia. [Peterson, Daniel G.] Mississippi State Univ, Dept Plant & Soil Sci, Starkville, MS USA. [Thaxton, Peggy] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS USA. [Jones, Don C.] Cotton Inc, Cary, NC USA. RP Zhang, TZ (reprint author), Nanjing Agr Univ, Cotton Hybrid R&D Engn Ctr, Minist Educ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China. EM cotton@njau.edu.cn; moelab@njau.edu.cn; lirq@novogene.cn; zjchen@austin.utexas.edu RI Llewellyn, Danny/D-5230-2009; Dennis, Elizabeth/A-6074-2008; OI Llewellyn, Danny/0000-0001-9535-8707; Chen, Xiaoya/0000-0002-2909-8414; Scheffler, Brian/0000-0003-1968-8952 FU Major State Basic Research Development Program of China (973 Program) [2011CB109300]; National Natural Science Foundation of China [3133058, 31290213]; NSF [ISO1025947]; Fundamental Research Funds by the Central Universities [KYZ201201]; Cotton Incorporated [07-161, 14-371]; Priority Academic Program Development of Jiangsu Higher Education Institutions; 111 program FX This work was financially supported in part by grants from the Major State Basic Research Development Program of China (973 Program) (2011CB109300), National Natural Science Foundation of China (No. 3133058; No. 31290213), NSF (ISO1025947), The Fundamental Research Funds by the Central Universities (KYZ201201), Cotton Incorporated (07-161, 14-371), the Priority Academic Program Development of Jiangsu Higher Education Institutions and the 111 program. NR 83 TC 149 Z9 176 U1 31 U2 120 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1087-0156 EI 1546-1696 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD MAY PY 2015 VL 33 IS 5 BP 531 EP U252 DI 10.1038/nbt.3207 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CH8VP UT WOS:000354314500035 PM 25893781 ER PT J AU Saito, K Cao, XH He, YL Xu, Y AF Saito, Kenji Cao, Xuehong He, Yanlin Xu, Yong TI Progress in the Molecular Understanding of Central Regulation of Body Weight by Estrogens SO OBESITY LA English DT Review ID CENTRAL MELANOCORTIN SYSTEM; NUCLEUS-TRACTUS-SOLITARIUS; ALPHA-EXPRESSING CELLS; DORSAL RAPHE NUCLEUS; HIGH-FAT DIET; RECEPTOR-ALPHA; FOOD-INTAKE; OVARIECTOMIZED RATS; FEMALE RATS; VENTROMEDIAL HYPOTHALAMUS AB ObjectiveEstrogens can act in the brain to prevent body weight gain. Tremendous research efforts have been focused on estrogen physiology in the brain in the context of body weight control; estrogen receptors and the related signals have been attractive targets for development of new obesity therapies. The objective is to review recent findings on these aspects. MethodsRecent studies that used conventional and conditional knockout mouse strains to delineate the cellular and molecular mechanisms for the beneficial effects of estrogens on body weight balance are reviewed. Emerging genetic tools that could further benefit the field of estrogen research and a newly developed estrogen-based regimen that produces body weight-lowering benefits also are discussed. ResultsThe body weight-lowering effects of estrogens are mediated by multiple forms of estrogen receptors in different brain regions through distinct but coordinated mechanisms. Both rapid signals and classic nuclear receptor actions of estrogen receptors appear to contribute to estrogenic regulation of body weight. ConclusionsEstrogen receptors and associated signal networks are potential targets for obesity treatment, and further investigations are warranted. C1 [Saito, Kenji; Cao, Xuehong; He, Yanlin; Xu, Yong] Baylor Coll Med, Dept Pediat, USDA Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Xu, Yong] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. RP Xu, Y (reprint author), Baylor Coll Med, Dept Pediat, USDA Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM yongx@bcm.edu OI He, Yanlin/0000-0002-5471-9016 FU NIH [R01DK093587, R01DK101379]; USDA ARS/CRIS [6250-51000-055] FX Funding agencies: NIH (R01DK093587 and R01DK101379) and USDA ARS/CRIS (6250-51000-055). NR 74 TC 5 Z9 5 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD MAY PY 2015 VL 23 IS 5 BP 919 EP 926 DI 10.1002/oby.21099 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA CH3XL UT WOS:000353964200003 PM 25865677 ER PT J AU Anzman-Frasca, S Mueller, MP Sliwa, S Dolan, PR Harelick, L Roberts, SB Washburn, K Economos, CD AF Anzman-Frasca, Stephanie Mueller, Megan P. Sliwa, Sarah Dolan, Peter R. Harelick, Linda Roberts, Susan B. Washburn, Kyle Economos, Christina D. TI Changes in Children's Meal Orders Following Healthy Menu Modifications at a Regional US Restaurant Chain SO OBESITY LA English DT Article ID FAST-FOOD; CHILDHOOD OBESITY; NUTRIENT-INTAKE; 14-YEAR TRENDS; ENERGY; ADOLESCENTS; PREFERENCES; QUALITY; CHOICE; ASSOCIATIONS AB ObjectiveTo examine changes in children's meal orders, price, and revenue following the implementation of a healthier children's menu in a full-service restaurant chain. MethodsIn April 2012, the healthier menu was implemented, featuring more meals meeting nutrition standards, healthy side dishes by default, and removal of French fries and soda (which could be substituted). Orders (n=352,192) were analyzed before (September 2011 to March 2012; PRE) and after (September 2012 to March 2013; POST) implementation. ResultsChildren's meal prices increased by $0.79 for breakfasts and $0.19 for non-breakfast meals from PRE to POST. Revenue continued to increase post-implementation. Orders of healthy meals, strawberry and vegetable sides, milk, and juice increased, and orders of French fries and soda decreased (P<0.0001). Orders at POST were more likely to include healthy sides (P<0.0001) and substitutions (P<0.0001) and less likely to include a la carte sides (P<0.0001) and desserts (P<0.01), versus PRE. Total calories ordered by children accepting all defaults decreased (684.2 vs. 621.2; P<0.0001) and did not change for those not accepting defaults (935.0 vs. 942.9; P=0.57). ConclusionsHealthy children's menu modifications were accompanied by healthier ordering patterns, without removing choice or reducing revenue, suggesting that they can improve child nutrition while restaurants remain competitive. C1 [Anzman-Frasca, Stephanie; Dolan, Peter R.; Harelick, Linda; Washburn, Kyle; Economos, Christina D.] Tufts Univ, ChildObesity180, Boston, MA 02111 USA. [Anzman-Frasca, Stephanie; Mueller, Megan P.; Sliwa, Sarah; Economos, Christina D.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Anzman-Frasca, S (reprint author), Tufts Univ, ChildObesity180, Boston, MA 02111 USA. EM stephanie.anzman_frasca@tufts.edu FU Robert Wood Johnson Foundation; JPB Foundation FX Funding agencies: This research was supported by Robert Wood Johnson Foundation and JPB Foundation. NR 30 TC 13 Z9 13 U1 1 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD MAY PY 2015 VL 23 IS 5 BP 1055 EP 1062 DI 10.1002/oby.21061 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA CH3XL UT WOS:000353964200020 PM 25919925 ER PT J AU Homer, C Dewitz, J Yang, LM Jin, S Danielson, P Xian, G Coulston, J Herold, N Wickham, J Megown, K AF Homer, Collin Dewitz, Jon Yang, Limin Jin, Suming Danielson, Patrick Xian, George Coulston, John Herold, Nathaniel Wickham, James Megown, Kevin TI Completion of the 2011 National Land Cover Database for the Conterminous United States - Representing a Decade of Land Cover Change Information SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID IMPERVIOUS SURFACE; THEMATIC ACCURACY; CANOPY COVER; IMAGERY C1 [Homer, Collin; Dewitz, Jon; Yang, Limin; Jin, Suming; Danielson, Patrick; Xian, George] US Geol Survey, Earth Resources Observat & Sci EROS Ctr, Garretson, SD 57030 USA. [Yang, Limin; Danielson, Patrick] Stinger Ghaffarian Technol, Houston, TX USA. [Jin, Suming; Xian, George] InuTeq, Baarn, Netherlands. [Coulston, John] US Forest Serv, Forest Inventory & Anal, Washington, DC USA. [Herold, Nathaniel] NOAA, Coastal Serv Ctr, San Juan, PR USA. [Megown, Kevin] US Forest Serv, Remote Sensing Applicat Ctr, Washington, DC USA. RP Homer, C (reprint author), US Geol Survey, Earth Resources Observat & Sci EROS Ctr, Garretson, SD 57030 USA. OI Dewitz, Jon/0000-0002-0458-212X FU U.S. Geological Survey [G10PC00044, G08PC91508]; United States Environmental Protection Agency (US EPA), through Office of Research and Development FX Research, development, and production efforts for NLCD 2011 data products and supplementary layers result from the cooperative efforts of several teams of dedicated individuals. Because of the number of individuals involved, they cannot all be properly acknowledged here. We would like to acknowledge the many organizations that made this work possible especially the support of the individuals and agencies of the MRLC Consortium, in addition to Federal and Federal contractor mapping teams. This study was made possible in part by SGT under U.S. Geological Survey contract G10PC00044 and by ASRC under U.S. Geological Survey contract G08PC91508. The United States Environmental Protection Agency (US EPA), through its Office of Research and Development, partly funded the research described here. It has been subject to Agency review and approved for publication. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 22 TC 157 Z9 159 U1 13 U2 62 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD MAY PY 2015 VL 81 IS 5 BP 345 EP 354 DI 10.14358/PERS.81.5.345 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA CH4OA UT WOS:000354011600002 ER PT J AU Gillin, CP Bailey, SW McGuire, KJ Prisley, SP AF Gillin, Cody P. Bailey, Scott W. McGuire, Kevin J. Prisley, Stephen P. TI Evaluation of Lidar-derived DEMs through Terrain Analysis and Field Comparison SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID DIGITAL ELEVATION MODELS; ACCURACY; RESOLUTION; FOREST; SLOPE; SCALE; ATTRIBUTES; CATCHMENT; HYDROLOGY; DENSITY AB Topographic analysis of watershed-scale soil and hydrological processes using digital elevation models (DEMs) is commonplace, but most studies have used DEMs of 10 m resolution or coarser. Availability of higher-resolution DEMs created from light detection and ranging (lidar) data is increasing but their suitability for such applications has received little critical evaluation. Two different 1 m DEMs were re-sampled to 3, 5, and 10 m resolutions and used with and without a low-pass smoothing filter to delineate catchment boundaries and calculate topographic metrics. Accuracy was assessed through comparison with field slope measurements and total station surveys. DEMs provided a good estimate of slope values when grid resolution reflected the field measurement scale. Intermediate scale DEMs were most consistent with land survey techniques in delineating catchment boundaries. Upslope accumulated area was most sensitive to grid resolution, with intermediate resolutions producing a range of UAA values useful in soil and groundwater analysis. C1 [Gillin, Cody P.] Trout Unlimited, Wenatchee, WA 98801 USA. [Gillin, Cody P.; McGuire, Kevin J.; Prisley, Stephen P.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Bailey, Scott W.] USDA, Forest Serv, No Res Stn, North Woodstock, NH USA. [McGuire, Kevin J.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA USA. RP Gillin, CP (reprint author), Trout Unlimited, Wenatchee, WA 98801 USA. EM swbailey@fs.fed.us RI McGuire, Kevin/E-7770-2010; OI McGuire, Kevin/0000-0001-5751-3956; Bailey, Scott/0000-0002-9160-156X FU National Science Foundation [DEB 1114804]; Hydrologic Sciences [EAR 1014507]; Research Experience for Undergraduate program [DBI/EAR 0754678]; National Center for Airborne Laser Mapping (NCALM) FX Financial support was provided by the National Science Foundation Long-Term Ecological Research (DEB 1114804), Hydrologic Sciences (EAR 1014507), and Research Experience for Undergraduate (DBI/EAR 0754678) programs. Lidar data were collected by Photo Science, Inc. for the White Mountain National Forest and as part of the National Center for Airborne Laser Mapping (NCALM) seed award program. Field work was partially conducted by Rebecca Bourgault, Margaret Burns, Erin Shoop-Volitis, J.P. Gannon, and Geoffrey Schwaner. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, Pennsylvania and is part of the NSF Long-Term Ecological Research network. NR 44 TC 3 Z9 3 U1 0 U2 13 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD MAY PY 2015 VL 81 IS 5 BP 387 EP 396 DI 10.14358/PERS.81.5.387 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA CH4OA UT WOS:000354011600008 ER PT J AU Mahmoud, UT Abdel-Rahman, MAM Darwish, MHA Applegate, TJ Cheng, HW AF Mahmoud, Usama Taha Abdel-Rahman, Mootaz Ahmed Mohamed Darwish, Madeha Hosni Ahmed Applegate, Todd Jay Cheng, Heng-Wei TI Behavioral changes and feathering score in heat stressed broiler chickens fed diets containing different levels of propolis SO APPLIED ANIMAL BEHAVIOUR SCIENCE LA English DT Article DE Broiler; Heat stress; Propolis; Behavior; Panting; Feather ID LAYING HENS; FEED-INTAKE; ANTIOXIDANT ACTIVITY; BRAZILIAN PROPOLIS; PLANT-ORIGIN; PERFORMANCE; TEMPERATURE; GROWTH; STRAINS; WEIGHT AB This experiment was conducted to evaluate the effects of dietary supplementation of green Brazilian propolis on behavioral patterns and feather condition of heat stressed broiler chickens. Five hundred and four (504) male Ross 708 broiler chicks at 15-day old were randomly allotted to six dietary treatments containing 0 (control), 100, 250, 500, 1000 and 3000 mg kg(-1) propolis, respectively. Each treatment consisted of four replicates of 21 birds each. Heat stress at 32 degrees C was applied daily from 08:00 am to 17:00 pm from day 15-42. Twelve birds per treatment were randomly taken and marked with livestock color for behavioral observation. Treatment effects on behavioral patterns were recorded using direct observation instantaneous scan sampling technique. At the end of the experiment (42 days of age) the three birds used for behavioral observation were feather scored (from 1 to 5); while all birds (504) were individually scanned for primary feather abnormalities. The results indicated that the effects of using propolis as a feed additive for broilers reduced heat stress behavior, especially dietary treatment with 250 or 3000 mg kg(-1) propolis, by increasing bird walking activities and reducing panting behaviors (P < 0.05, respectively). Moreover, 250 mg kg(-1) propolis (P < 0.05) increased standing behavior also. Walking activities was also increased in the birds fed with 100 mg kg(-1) propolis (P < 0.05). There were no significant (P > 0.05) treatment effects on sitting, preening, feeding, drinking, wing elevations and feather pecking activities. Propolis at current doses did not significantly (P > 0.05) improve feather scoring, but reduced (P = 0.048) abnormal shape of primary feathers. The current results support that propolis treatment may be considered as a protective management practice in broiler chickens to alleviate the negative effects of heat stress, but further investigations may requested to determine the best type, dose, time and duration of its administration. (C) 2015 Elsevier B.V. All rights reserved. C1 [Mahmoud, Usama Taha; Abdel-Rahman, Mootaz Ahmed Mohamed; Darwish, Madeha Hosni Ahmed] Assiut Univ, Fac Vet Med, Dept Anim Hyg, Assiut 71526, Egypt. [Mahmoud, Usama Taha; Applegate, Todd Jay] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Cheng, Heng-Wei] Purdue Univ, USDA ARS, W Lafayette, IN 47907 USA. RP Mahmoud, UT (reprint author), 125 S Russell St, W Lafayette, IN 47907 USA. EM usamakomsan5@gmail.com OI Applegate, Todd/0000-0002-2406-5058; mahmoud, usama/0000-0001-8650-2420 FU Egyptian Cultural and Educational Bureau (ECEB) in Washington DC., Ministry of Higher Education and Scientific Research, Egypt FX Research reported in this publication was supported by the Egyptian Cultural and Educational Bureau (ECEB) in Washington DC., Ministry of Higher Education and Scientific Research, Egypt. NR 39 TC 2 Z9 2 U1 4 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1591 EI 1872-9045 J9 APPL ANIM BEHAV SCI JI Appl. Anim. Behav. Sci. PD MAY PY 2015 VL 166 BP 98 EP 105 DI 10.1016/j.applanim.2015.03.003 PG 8 WC Agriculture, Dairy & Animal Science; Behavioral Sciences; Veterinary Sciences SC Agriculture; Behavioral Sciences; Veterinary Sciences GA CG9CZ UT WOS:000353613300012 ER PT J AU Solaiman, DKY Ashby, RD Gunther, NW Zerkowski, JA AF Solaiman, Daniel K. Y. Ashby, Richard D. Gunther, Nereus W. Zerkowski, Jonathan A. TI Dirhamnose-lipid production by recombinant nonpathogenic bacterium Pseudomonas chlororaphis SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Biosurfactant; Microbial glycolipid; Pseudomonas chlororaphis; Rhamnolipid; Rhamnosyltransferases; Surface tension ID 3-(3-HYDROXYALKANOYLOXY)ALKANOIC ACIDS HAAS; SURFACE-ACTIVE PROPERTIES; BETA-KETOACYL REDUCTASE; DE-NOVO BIOSYNTHESIS; RHAMNOLIPID PRODUCTION; ESCHERICHIA-COLI; POLYHYDROXYALKANOATE BIOSYNTHESIS; FUNCTIONAL-CHARACTERIZATION; PUTIDA KT2440; AERUGINOSA AB We previously discovered that Pseudomonas chlororaphis NRRL B-30761 produces monorhamnolipids (R(1)Ls) with predominantly 3-hydroxydodecenoyl-3-hydroxydecanoate (C-12:1-C-10) or 3-hydroxydodecanoyl-3-hydroxydecanoate (C-12-C-10) as the lipid moiety under static growth conditions only. We have now cloned, sequenced, and analyzed in silico the gene locus of NRRL B-30761 containing the putative coding sequences of rhamnosyltransferase chain A (rhlA (Pch) , 894 bps), rhamnosyltransferase chain B (rhlB (Pch) , 1272 bps), and N-acyl-homoserine lactone-dependent transcriptional regulatory protein (rhlR (Pch) , 726 bps). The putative gene products RhlA(Pch) (297 amino acid residues or a.a.), RhlB(Pch) (423 a.a.), and RhlR(Pch) (241 a.a.) only have between 60 and 65 % a.a. identities to their respective closest matched homologs in P. aeruginosa. Polymerase chain reaction (PCR)-based assay did not detect the presence of rhamnosyltransferase C gene (rhlC) in P. chlororaphis, suggesting a genetic basis for the lack of dirhamnose-lipid (R2L) synthesis in this organism. We thus genetically constructed an R2L-synthesizing P. chlororaphis by expressing a rhamnosyltransferase C (rhlC) gene of P. aeruginosa using an expression vector (pBS29-P2-gfp) containing a Pseudomonas syringae promoter. The R2L/R1L ratio is 2.4 in the rhamnolipid (RL) sample isolated from the genetically engineered (GE) P. chlororaphis [pBS29-P2-rhlC], in contrast to undetectable R2L in the GE P. chlororaphis [pBS29-P2-gfp] control cells based on LC-MS analysis. The critical micelle concentrations of the R2L and R1L samples from GE P. chlororaphis [pBS29-P2-rhlC] and the control [pBS29-P2-gfp] cells were ca. 0.1 mM, and their minimum surface tensions were ca. 26 mN/m with no significant difference. C1 [Solaiman, Daniel K. Y.; Ashby, Richard D.; Gunther, Nereus W.; Zerkowski, Jonathan A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Solaiman, DKY (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM dan.solaiman@ars.usda.gov NR 57 TC 2 Z9 2 U1 2 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD MAY PY 2015 VL 99 IS 10 BP 4333 EP 4342 DI 10.1007/s00253-015-6433-4 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CH2AQ UT WOS:000353826200017 PM 25661819 ER PT J AU Langwig, KE Voyles, J Wilber, MQ Frick, WF Murray, KA Bolker, BM Collins, JP Cheng, TL Fisher, MC Hoyt, JR Lindner, DL McCallum, HI Puschendorf, R Rosenblum, EB Toothman, M Willis, CKR Briggs, CJ Kilpatrick, AM AF Langwig, Kate E. Voyles, Jamie Wilber, Mark Q. Frick, Winifred F. Murray, Kris A. Bolker, Benjamin M. Collins, James P. Cheng, Tina L. Fisher, Matthew C. Hoyt, Joseph R. Lindner, Daniel L. McCallum, Hamish I. Puschendorf, Robert Rosenblum, Erica Bree Toothman, Mary Willis, Craig K. R. Briggs, Cheryl J. Kilpatrick, A. Marm TI Context-dependent conservation responses to emerging wildlife diseases SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID WEST-NILE-VIRUS; FACIAL TUMOR DISEASE; WHITE-NOSE SYNDROME; PATHOGEN; TRANSMISSION; CHYTRIDIOMYCOSIS; POPULATIONS; STRATEGIES; MANAGEMENT; SPREAD AB Emerging infectious diseases pose an important threat to wildlife. While established protocols exist for combating outbreaks of human and agricultural pathogens, appropriate management actions before, during, and after the invasion of wildlife pathogens have not been developed. We describe stage-specific goals and management actions that minimize disease impacts on wildlife, and the research required to implement them. Before pathogen arrival, reducing the probability of introduction through quarantine and trade restrictions is key because prevention is more cost effective than subsequent responses. On the invasion front, the main goals are limiting pathogen spread and preventing establishment. In locations experiencing an epidemic, management should focus on reducing transmission and disease, and promoting the development of resistance or tolerance. Finally, if pathogen and host populations reach a stable stage, then recovery of host populations in the face of new threats is paramount. Successful management of wildlife disease requires risk-taking, rapid implementation, and an adaptive approach. C1 [Langwig, Kate E.; Frick, Winifred F.; Cheng, Tina L.; Hoyt, Joseph R.; Kilpatrick, A. Marm] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Voyles, Jamie] New Mexico Inst Min & Technol, Dept Biol, Socorro, NM 87801 USA. [Wilber, Mark Q.; Toothman, Mary; Briggs, Cheryl J.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Murray, Kris A.] Univ London Imperial Coll Sci Technol & Med, Grantham Inst Climate Change & Environm, London, England. [Murray, Kris A.] EcoHlth Alliance, New York, NY USA. [Bolker, Benjamin M.] McMaster Univ, Dept Math & Stat, Hamilton, ON, Canada. [Bolker, Benjamin M.] McMaster Univ, Dept Biol, Hamilton, ON, Canada. [Collins, James P.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Fisher, Matthew C.] Univ London Imperial Coll Sci Technol & Med, Dept Infect Dis Epidemiol, London, England. [Lindner, Daniel L.] US Forest Serv, Ctr Mycol Res, Madison, WI USA. [McCallum, Hamish I.] Griffith Univ, Sch Environm, Nathan, Qld 4111, Australia. [Puschendorf, Robert] Univ Plymouth, Sch Biol Sci, Plymouth PL4 8AA, Devon, England. [Rosenblum, Erica Bree] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Willis, Craig K. R.] Univ Winnipeg, Dept Biol, Winnipeg, MB R3B 2E9, Canada. RP Langwig, KE (reprint author), Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. EM klangwig@gmail.com RI Puschendorf, Robert/B-6280-2011; McCallum, Hamish/E-1638-2013; OI Puschendorf, Robert/0000-0002-8105-171X; McCallum, Hamish/0000-0002-3493-0412; Fisher, Matthew/0000-0002-1862-6402; Bolker, Benjamin/0000-0002-2127-0443 FU US National Science Foundation [EF-0914866, DGE-0741448, DEB-1115069, DEB-1336290]; National Institutes of Health [1R010AI090159] FX Ideas for this paper were developed during the National Center for Ecological Analysis and Synthesis working group "Fungal pathogens and disease-induced extinction: are fungal diseases different?". Funding was provided by the US National Science Foundation (grants EF-0914866, DGE-0741448, DEB-1115069, DEB-1336290) and the National Institutes of Health (grant 1R010AI090159). NR 51 TC 22 Z9 22 U1 15 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD MAY PY 2015 VL 13 IS 4 BP 195 EP 202 DI 10.1890/140241 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CH4KJ UT WOS:000354002100014 ER PT J AU Sousa, AMM Souza, HKS Liu, LS Goncalves, MP AF Sousa, Ana M. M. Souza, Hileia K. S. Liu, LinShu Goncalves, Maria P. TI Alternative plasticizers for the production of thermo-compressed agar films SO INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES LA English DT Article DE Agar; Urea; Choline chloride; Mechanical properties; Response surface methodology; Central composite rotatable design ID RESPONSE-SURFACE METHODOLOGY; MECHANICAL-PROPERTIES; MORPHOLOGICAL PROPERTIES; NANOCOMPOSITE FILM; BIOPOLYMER FILMS; FOOD; OPTIMIZATION; EXTRACTION; FABRICATION; STARCH AB Agar films were produced by thermo-compression using choline chloride (ChCl) as a plasticizer with urea. The three solid components were mixed together with the salt and urea (minor components) added to agar (main component) according to a fixed mass ratio of, respectively, 1.16:1:5. A central composite rotatable design (CCRD) with three parameters, 23, was used to evaluate the effects of temperature (Xi; degrees C), time (X-2; min) and applied load (X-3; kN) of heat-pressing on the maximum tensile strength (TS) of the films (Y; MPa). Mixtures of urea and agar prepared at a mass ratio of 1:5 did not form homogeneous films suggesting the important plasticizing role of the salt. Heat-pressing the mixtures at more draconian conditions led to much darker and opaque films, with better mechanical resistance (higher values of TS). The most resistant film (similar to 15 MPa) was obtained at 140 degrees C, 20 min and 176 kN. Selected films, including the optimal, showed similar water sorption profiles and close values of water vapor permeability (similar to 2.5-3.7 x 10(-9) gm(-1) s(-1) Pa-1). The fracture behavior and mechanical properties of the films were greatly affected by additional water plasticization when the films were stored at different conditions of relative humidity. (C) 2015 Elsevier B.V. All rights reserved. C1 [Sousa, Ana M. M.; Souza, Hileia K. S.; Goncalves, Maria P.] Univ Porto, Fac Engn, REQUIMTE LAQV, P-4200465 Oporto, Portugal. [Sousa, Ana M. M.; Liu, LinShu] ARS, Dairy & Funct Foods Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Goncalves, MP (reprint author), Univ Porto, Fac Engn, REQUIMTE LAQV, Rua Dr Roberto Frias, P-4200465 Oporto, Portugal. EM ana.sousa@ars.usda.gov; hileiak@yahoo.com; LinShu.Liu@ars.usda.gov; pilarg@fe.up.pt RI Souza, Hileia/D-4739-2013; Goncalves, Maria/D-4641-2013 OI Souza, Hileia/0000-0003-2619-6892; Goncalves, Maria/0000-0002-8873-4097 FU FEDER funds through the Operational Program for Competitiveness Factors - COMPETE; National Funds through FCT - Foundation for Science and Technology [Pest-C/EQB/LA0006/2013-FCOMP-01-0124-FEDER-37285] FX This work was funded by FEDER funds through the Operational Program for Competitiveness Factors - COMPETE and by National Funds through FCT - Foundation for Science and Technology under the project Pest-C/EQB/LA0006/2013-FCOMP-01-0124-FEDER-37285. To all financing sources the authors are greatly indebted. Joseph Uknalis (USDA, ARS, ERRC, Wydmoor, PA) is also acknowledged for SEM analyses. NR 41 TC 2 Z9 2 U1 4 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0141-8130 EI 1879-0003 J9 INT J BIOL MACROMOL JI Int. J. Biol. Macromol. PD MAY PY 2015 VL 76 BP 138 EP 145 DI 10.1016/j.ijbiomac.2015.02.030 PG 8 WC Biochemistry & Molecular Biology; Chemistry, Applied; Polymer Science SC Biochemistry & Molecular Biology; Chemistry; Polymer Science GA CG8ZK UT WOS:000353604000018 PM 25727746 ER PT J AU Ukaew, S Beck, E Archer, DW Shonnard, DR AF Ukaew, Suchada Beck, Emily Archer, David W. Shonnard, David R. TI Estimation of soil carbon change from rotation cropping of rapeseed with wheat in the hydrotreated renewable jet life cycle SO INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT LA English DT Article DE Hydrotreated renewable jet; IPCC; Land use change; Life cycle assessment; Rapeseed rotation; Soil carbon change ID ORGANIC-MATTER; CANADIAN PRAIRIE; EMISSION FACTORS; GREAT-PLAINS; SYSTEMS; BIODIESEL; BIOFUELS; TILLAGE; ENERGY; PRODUCTIVITY AB Rapeseed is being considered as a potential feedstock for hydrotreated renewable jet (HRJ) fuel in the USA through its cultivation in rotation with wheat. The goal of this research was to determine the impact of soil C changes, induced through replacing the fallow period with rapeseed in rotation with wheat, and the effects it would have on emission of greenhouse gases (GHG) of rapeseed HRJ. The Intergovernmental Panel on Climate Change (IPCC) (Tier 1) method was used with modifications to determine the changes in soil C of wheat-wheat-rapeseed (WWR) relative to the reference wheat-wheat-fallow (WWF) rotation for 20 years of cultivation. The 27 case scenarios were conducted to study the impacts of changes in management practices (tillage practice and residue input) on changes in soil C for WWR rotation in multiple locations in 10 US states. The CO2 emissions resulting from soil C changes were incorporated into the rapeseed HRJ pathway in order to evaluate the GHG emissions. Introducing rapeseed to replace the fallow period with wheat could either increase or decrease changes in soil C, depending on management practices. Soil C is predicted to increase with increased residue input and reduced tillage. The greatest gain of soil C was found when using high residue input for wheat and rapeseed under no tillage, resulting in the best management practice. Conversely, adding low residue input to both crops with full tillage created the highest loss of soil C, referring to as the worst management practice. Soil C changes varied across locations from -0.22 to 0.32 Mg C ha(-1) year(-1). Consequently, the GHG emissions of rapeseed HRJ ranged from 4 to 70 g CO2 eq./MJ, comparing to 46 g CO2 eq./MJ for excluding soil C change. The rapeseed HRJ exhibited the GHG savings of 65-96 % for the best practice and 20-42 % for the worst practice when compared to petroleum jet fuel. Based on results using the modified IPCC method, adoption of high residue input with no tillage for the rotation cropping of rapeseed with wheat had the potential to increase soil C. However, the method has limitations for predicting soil C changes regarding crop management practices. Biogeochemical-based models that have a potential to capture processes of C and N dynamics in soil and yield may be better suited to quantify regional variations in soil C changes for the rotation cropping of rapeseed with wheat. C1 [Ukaew, Suchada; Beck, Emily; Shonnard, David R.] Michigan Technol Univ, Dept Chem Engn, Houghton, MI 49931 USA. [Archer, David W.] ARS, USDA, Mandan, ND 58554 USA. [Shonnard, David R.] Michigan Technol Univ, Sustainable Futures Inst, Houghton, MI 49931 USA. RP Ukaew, S (reprint author), Michigan Technol Univ, Dept Chem Engn, Houghton, MI 49931 USA. EM sukaew@mtu.edu FU USDA-ARS [59-3620-3-002]; NIFA BRDI [60-3620-2-361/USDA, 2012-10008-19727]; Royal Thai Government Scholarship program FX We greatly appreciate the contribution of Dr. Joon Hee Lee (USDA ARS) for providing soil and weather information. Funding from the Agreement No. 59-3620-3-002 on USDA-ARS Interagency Agreement No. 60-3620-2-361/USDA NIFA BRDI No. 2012-10008-19727 is appreciated. Ms. Suchada Ukaew is supported by a grant from the Royal Thai Government Scholarship program. The Richard and Bonnie Robbins endowment is also appreciated. NR 72 TC 1 Z9 1 U1 4 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0948-3349 EI 1614-7502 J9 INT J LIFE CYCLE ASS JI Int. J. Life Cycle Assess. PD MAY PY 2015 VL 20 IS 5 BP 608 EP 622 DI 10.1007/s11367-015-0863-1 PG 15 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CG8QF UT WOS:000353573800004 ER PT J AU Varadharajan, C Han, RY Beller, HR Yang, L Marcus, MA Michel, M Nico, PS AF Varadharajan, Charuleka Han, Ruyang Beller, Harry R. Yang, Li Marcus, Matthew A. Michel, Marc Nico, Peter S. TI Characterization of Chromium Bioremediation Products in Flow-Through Column Sediments Using Micro-X-ray Fluorescence and X-ray Absorption Spectroscopy SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CHROMATE REDUCTION; HEXAVALENT CHROMIUM; CR(VI) REDUCTION; FERROUS IRON; SURFACES; KINETICS; BACTERIA; IFEFFIT; SOIL AB Microbially mediated reductive immobilization of chromium is a possible remediation technique for sites contaminated with Cr(VI). This study is part of a broader effort investigating the biogeochemical mechanisms for Cr(VI) reduction in Hanford 100H aquifer sediments using flow-through laboratory columns. It had previously been shown that reduced chromium in the solid phase was in the form of freshly precipitated mixed-phase Cr(III)-Fe(III) (hydr)oxides, irrespective of the biogeochemical conditions in the columns. In this study, the reduced Cr phases in the columns were investigated further using spectroscopy to understand the structure and mechanisms involved in the formation of the end products. Several samples representing potential processes that could be occurring in the columns were synthesized in the laboratory and characterized using X-ray absorption near edge structure (XANES) and X-ray scattering. The XANES of Cr(III) particles in the columns most closely resembled those from synthetic samples produced by the abiotic reaction of Cr(VI) with microbially reduced Fe(II). Microbially mediated Cr-Fe reduction products were distinct from abiotic Cr-Fe (hydr) oxides [CrxFe1-x(OH)(3)] and organically complexed Cr(III) sorbed onto the surface of a mixed ferrihydrite-goethite mineral phase. Furthermore, analyses of the abiotically synthesized samples revealed that even the end products of purely abiotic, iron-mediated reduction of Cr(VI) are affected by factors such as the presence of excess aqueous Fe(II) and cellular matter. These results suggest that CrxFe1-x(OH)(3) phases made under realistic subsurface conditions or in biotic cultures are structurally different from pure Cr(OH)(3) or laboratory-synthesized CrxFe1-x(OH)(3). The observed structural differences imply that the reactivity and stability of biogenic CrxFe1-x(OH)(3) could potentially be different from that of abiotic CrxFe1-x(OH)(3). C1 [Varadharajan, Charuleka; Han, Ruyang; Beller, Harry R.; Yang, Li; Nico, Peter S.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA. [Han, Ruyang] Univ Calif Davis, Dept Plant Sci, USDA ARS, Albany, CA 94710 USA. [Marcus, Matthew A.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Adv Light Source, Berkeley, CA 94720 USA. [Michel, Marc] Virginia Tech, Dept Geosci, Blacksburg, VA 24061 USA. RP Varadharajan, C (reprint author), Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, 1 Cyclotron Rd, Berkeley, CA 94720 USA. EM cvaradharajan@lbl.gov RI Beller, Harry/H-6973-2014; Nico, Peter/F-6997-2010; YANG, LI/F-9392-2010; Varadharajan, Charuleka/G-3741-2015 OI Nico, Peter/0000-0002-4180-9397; Varadharajan, Charuleka/0000-0002-4142-3224 FU Sustainable Systems Scientific Focus Area - US Department of Energy, Office of Science, Office of Biological and Environmental Resources [DE-AC02-05CH11231]; Office of Science, Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231] FX This work was supported as part of the Sustainable Systems Scientific Focus Area funded by the US Department of Energy, Office of Science, Office of Biological and Environmental Resources under Award Number DE-AC02-05CH11231. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the US Department of Energy under contract no. DE-AC02-05CH11231. Spectroscopic data were also collected at the Stanford Synchrotron Radiation Lightsource, a Directorate of SLAC National Accelerator Laboratory and an Office of Science User Facility operated for the US Department of Energy Office of Science by Stanford University. The authors thank Sirine Fakra, Ruth Tinnacher, Hiram-Castillo Michel, Mike Massey, John Bargar, Sam Webb, and Benjamin Kocar for help with the collection of synchrotron data; Joern Larsen and April Van Hise for conducting the ICP-MS analyses at LBNL; and thank associate editor Markus Grafe and the two anonymous reviewers who provided helpful comments for revising the manuscript. NR 36 TC 4 Z9 4 U1 1 U2 27 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 729 EP 738 DI 10.2134/jeq2014.08.0329 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200003 PM 26024254 ER PT J AU Tomer, MD Porter, SA Boomer, KMB James, DE Kostel, JA Helmers, MJ Isenhart, TM McLellan, E AF Tomer, M. D. Porter, S. A. Boomer, K. M. B. James, D. E. Kostel, J. A. Helmers, M. J. Isenhart, T. M. McLellan, E. TI Agricultural Conservation Planning Framework: 1. Developing Multipractice Watershed Planning Scenarios and Assessing Nutrient Reduction Potential SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CONSTRUCTED WETLANDS; FILTER STRIPS; TILE DRAINAGE; NITRATE; NITROGEN; REMOVAL; RIVER; PHOSPHORUS; LANDSCAPE; RUNOFF AB Spatial data on soils, land use, and topography, combined with knowledge of conservation effectiveness, can be used to identify alternatives to reduce nutrient discharge from small (hydrologic unit code [HUC]12) watersheds. Databases comprising soil attributes, agricultural land use, and light detection and ranging-derived elevation models were developed for two glaciated midwestern HUC12 watersheds: Iowa's Beaver Creek watershed has an older dissected landscape, and Lime Creek in Illinois is young and less dissected. Subsurface drainage is common in both watersheds. We identified locations for conservation practices, including in-field practices (grassed waterways), edge-of-field practices (nutrient-removal wetlands, saturated buffers), and drainage-water management, by applying terrain analyses, geographic criteria, and cross-classifications to field-and watershed-scale geographic data. Cover crops were randomly distributed to fields without geographic prioritization. A set of alternative planning scenarios was developed to represent a variety of extents of implementation among these practices. The scenarios were assessed for nutrient reduction potential using a spreadsheet approach to calculate the average nutrient-removal efficiency required among the practices included in each scenario to achieve a 40% NO3-N reduction. Results were evaluated in the context of the Iowa Nutrient Reduction Strategy, which reviewed nutrient-removal efficiencies of practices and established the 40% NO3-N reduction as Iowa's target for Gulf of Mexico hypoxia mitigation by agriculture. In both test watersheds, planning scenarios that could potentially achieve the targeted NO3-N reduction but remove <5% of cropland from production were identified. Cover crops and nutrient removal wetlands were common to these scenarios. This approach provides an interim technology to assist local watershed planning and could provide planning scenarios to evaluate using watershed simulation models. A set of ArcGIS tools is being released to enable transfer of this mapping technology. C1 [Tomer, M. D.; Porter, S. A.; James, D. E.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Boomer, K. M. B.] Nature Conservancy, Bethesda, MD 20814 USA. [Kostel, J. A.] Wetlands Initiat, Chicago, IL 60604 USA. [Helmers, M. J.; Isenhart, T. M.] Iowa State Univ, Coll Agr & Life Sci, Ames, IA 50011 USA. [McLellan, E.] Environm Def Fund, Washington, DC 20009 USA. RP Tomer, MD (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM mark.tomer@ars.usda.gov FU USDA-NRCS FX This study was conducted by the USDA-ARS and was partly supported through a Conservation Innovation Grant awarded by the USDA-NRCS to the Environmental Defense Fund. The authors thank the North Central Region Water Network for agreeing to host the ACPF ArcGIS toolbox on its website (http://northcentralwater.org/acpf/). NR 40 TC 3 Z9 3 U1 13 U2 58 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 754 EP 767 DI 10.2134/jeq2014.09.0386 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200005 PM 26024256 ER PT J AU Tomer, MD Boomer, KMB Porter, SA Gelder, BK James, DE McLellan, E AF Tomer, M. D. Boomer, K. M. B. Porter, S. A. Gelder, B. K. James, D. E. McLellan, E. TI Agricultural Conservation Planning Framework: 2. Classification of Riparian Buffer Design Types with Application to Assess and Map Stream Corridors SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID EFFECTS ASSESSMENT PROJECT; WATER-QUALITY; PRECISION CONSERVATION; SOUTH FORK; SEDIMENT; IOWA; RESTORATION; STRIPS; RIVER; SOIL AB A watershed's riparian corridor presents opportunities to stabilize streambanks, intercept runoff, and influence shallow groundwater with riparian buffers. This paper presents a system to classify these riparian opportunities and apply them toward riparian management planning in hydrologic unit code 12 watersheds. In two headwater watersheds from each of three landform regions found in Iowa and Illinois, high-resolution (3-m grid) digital elevation models were analyzed to identify spatial distributions of surface runoff contributions and zones with shallow water tables (SWTs) (within 1.5 m of the channel elevation) along the riparian corridors. Results were tabulated, and a cross classification was applied. Classes of buffers include those primarily placed to (i) trap runoff and sediment, (ii) influence shallow groundwater, (iii) address both runoff and shallow groundwater, and (iv) maintain/improve stream bank stability. Riparian buffers occupying about 2.5% of these six watersheds could effectively intercept runoff contributions from 81 to 94% of the watersheds' contributing areas. However, extents of riparian zones where a narrow buffer (<10 m wide) would adequately intercept runoff but where >25 m width of buffer vegetation could root to a SWT varied according to landform region (p < 0.10). Yet, these wide-SWT riparian zones were widespread and occupied 23 to 53% of the lengths of stream banks among the six watersheds. The wide-SWT setting provides opportunities to reduce dissolved nutrients (particularly NO3-N) carried via groundwater. This riparian classification and mapping system is part of a ArcGIS toolbox and could provide a consistent basis to identify riparian management opportunities in Midwestern headwater catchments wherever high-resolution elevation data are available. C1 [Tomer, M. D.; Porter, S. A.; James, D. E.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50010 USA. [Boomer, K. M. B.] Nature Conservancy, Bethesda, MD 20814 USA. [Gelder, B. K.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [McLellan, E.] Environm Def Fund, Washington, DC 20009 USA. RP Tomer, MD (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50010 USA. EM mark.tomer@ars.usda.gov FU USDA-NRCS FX This study was conducted by the USDA-ARS and was partly supported through a Conservation Innovation Grant awarded by the USDA-NRCS to the Environmental Defense Fund. The authors thank the North Central Region Water Network for agreeing to host the ACPF ArcGIS toolbox on its website (http://northcentralwater.org/acpf/). NR 36 TC 2 Z9 2 U1 6 U2 38 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 768 EP 779 DI 10.2134/jeq2014.09.0387 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200006 PM 26024257 ER PT J AU Soni, B Bartelt-Hunt, SL Snow, DD Gilley, JE Woodbury, BL Marx, DB Li, X AF Soni, Bhavneet Bartelt-Hunt, Shannon L. Snow, Daniel D. Gilley, John E. Woodbury, Bryan L. Marx, David B. Li, Xu TI Narrow Grass Hedges Reduce Tylosin and Associated Antimicrobial Resistance Genes in Agricultural Runoff SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID SOLID-PHASE EXTRACTION; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; FILTER STRIPS; RAINFALL SIMULATOR; LAND APPLICATION; FECAL BACTERIA; TRACE ANALYSIS; PLOT-SCALE; SOIL AB Agricultural runoff from areas receiving livestock manure can potentially contaminate surface water with antimicrobials and antimicrobial resistance genes (ARGs). The objective of this study was to investigate the effectiveness of narrow grass hedges (NGHs) on reducing the transport of antimicrobials and ARGs in runoff after land application of swine manure slurry. Plot-scale rainfall simulation tests were conducted on 0.75 m by 4.0 m plots designed to test three treatment factors: manure amendment (control plots receiving no manure vs. amended plots receiving manure based on 3 times N requirement), NGH (plots with a NGH vs. plots without a NGH), and rainfall events (days 1-3). Runoff generated during three 30-min simulated rainfall events was sampled and analyzed for antimicrobials and ARGs. Manure amendment was responsible for the presence of antimicrobial tylosin (p < 0.0001) and tylosin resistance gene erm(B) (p < 0.0001) in runoff. Narrow grass hedges proved to be effective in reducing tylosin (p < 0.0001) and erm(B) (p < 0.0347) in runoff. Manure amendment was responsible for the introduction of tylosin (p < 0.0482) and erm(B) (p = 0.0128) into the soil; however, it had no significant impact on the abundance of the 16S rRNA gene in soil. Results from this study suggest that NGHs could be a best management practice to control the transport of antimicrobials and ARGs in agricultural runoff. C1 [Soni, Bhavneet; Bartelt-Hunt, Shannon L.; Li, Xu] Univ Nebraska, Dept Civil Engn, Lincoln, NE 68588 USA. [Woodbury, Bryan L.] USDA, Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Li, X (reprint author), Univ Nebraska, Dept Civil Engn, 844 N 16th St,N117 SEC Link, Lincoln, NE 68588 USA. EM xli4@unl.edu FU National Pork Board (NPB) [12-012] FX Funding for this project was provided in part by the National Pork Board (NPB #12-012). NR 52 TC 0 Z9 0 U1 2 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 895 EP 902 DI 10.2134/jeq2014.09.0389 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200016 PM 26024269 ER PT J AU Williams, MR Buda, AR Elliott, HA Collick, AS Dell, C Kleinman, PJA AF Williams, Mark R. Buda, Anthony R. Elliott, Herschel A. Collick, Amy S. Dell, Curtis Kleinman, Peter J. A. TI Linking Nitrogen Management, Seep Chemistry, and Stream Water Quality in Two Agricultural Headwater Watersheds SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NITRATE REMOVAL; LAND-USE; INTEGRATING PHOSPHORUS; HILLSLOPE-SCALE; RIPARIAN ZONES; SURFACE FLOW; GROUNDWATER; USA; CATCHMENT; DYNAMICS AB Riparian seepage zones in headwater agricultural watersheds represent important sources of nitrate-nitrogen (NO3-N) to surface waters, often connecting N-rich groundwater systems to streams. In this study, we examined how NO3-N concentrations in seep and stream water were affected by NO3-N processing along seep surface flow paths and by upslope applications of N from fertilizers and manures. The research was conducted in two headwater agricultural watersheds, FD36 (40 ha) and RS (45 ha), which are fed, in part, by a shallow fractured aquifer system possessing high (3-16 mg L-1) NO3-N concentrations. Data from in-seep monitoring showed that NO3-N concentrations generally decreased downseep (top to bottom), indicating that most seeps retained or removed a fraction of delivered NO3-N (16% in FD36 and 1% in RS). Annual mean N applications in upslope fields (as determined by yearly farmer surveys) were highly correlated with seep NO3-N concentrations in both watersheds (slope: 0.06; R-2 = 0.79; p < 0.001). Strong positive relationships also existed between seep and stream NO3-N concentrations in FD36 (slope: 1.01; R-2 = 0.79; p < 0.001) and in RS (slope: 0.64; R-2 = 0.80; p < 0.001), further indicating that N applications control NO3-N concentrations at the watershed scale. Our findings clearly point to NO3-N leaching from upslope agricultural fields as the primary driver of NO3-N losses from seeps to streams in these watersheds and therefore suggest that appropriate management strategies (cover crops, limiting fall/winter nutrient applications, decision support tools) be targeted in these zones. C1 [Williams, Mark R.; Elliott, Herschel A.] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. [Buda, Anthony R.; Collick, Amy S.; Dell, Curtis; Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Williams, MR (reprint author), USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM mark.williams2@ars.usda.gov NR 52 TC 2 Z9 2 U1 1 U2 30 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 910 EP 920 DI 10.2134/jeq2014.10.0412 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200018 PM 26024271 ER PT J AU Ducey, TF Miller, JO Lang, MW Szogi, AA Hunt, PG Fenstermacher, DE Rabenhorst, MC McCarty, GW AF Ducey, T. F. Miller, J. O. Lang, M. W. Szogi, A. A. Hunt, P. G. Fenstermacher, D. E. Rabenhorst, M. C. McCarty, G. W. TI Soil Physicochemical Conditions, Denitrification Rates, and nosZ Abundance in North Carolina Coastal Plain Restored Wetlands SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID PAIRED NATURAL WETLANDS; SWINE WASTE-WATER; MICROBIAL COMMUNITY STRUCTURE; CONSTRUCTED WETLANDS; SPATIAL VARIABILITY; ORGANIC-MATTER; RESTORATION; GROUNDWATER; QUALITY; USA AB Over the last century, North Carolina has seen a severe reduction in the percentage of wetlands and a rise in negative environmental impacts related to this loss. To counter these effects, efforts have been enacted to mitigate wetland loss and create new wetland areas. The objective of this study was to assess the impact of hydrological restoration at several sites in the North Carolina coastal plain. Nine sites were selected for study. Hydrologically restored wetlands were compared with natural wetlands and prior converted (PC) croplands (i.e., historic wetlands under agricultural production). Each site was analyzed along a relative wetness gradient, and physicochemical properties, denitrification enzyme activity, and N2O reductase gene (nosZ) abundances using real-time PCR were measured. Physicochemically, restoration resulted in significantly increased levels of total C as compared with PC cropland sites. Restored wetland sites also saw pH, soil moisture, P, and NO2- +NO3- approximate levels similar to those of natural wetlands. Denitrification enzyme activity rates varied based on relative wetness within individual sites, generally increasing with increasing soil moisture. However, denitrification tended to be lower in restored wetland sites relative to natural wetlands. Gene abundances of nosZ saw statistically significant decreases in restored wetland soils. In conclusion, although analysis of restored wetlands reveals clear changes in several physicochemical characteristics and significant decreases in nosZ gene abundances, restoration efforts appear to have not significantly affected the denitrification component of the N cycle. C1 [Ducey, T. F.; Miller, J. O.; Szogi, A. A.; Hunt, P. G.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Lang, M. W.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Fenstermacher, D. E.; Rabenhorst, M. C.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [McCarty, G. W.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Ducey, TF (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM thomas.ducey@ars.usda.gov FU USDA-NRCS Wetland Component of the National Conservation Effects Assessment Project FX This research was supported by the USDA-NRCS Wetland Component of the National Conservation Effects Assessment Project. The authors thank W. Brigman, K.L. Lewis, and A.S. Shriner for technical support; Kenneth C. Stone and Philip J. Bauer for statistical support; and the property owners for their cooperation with this study and allowing access to field sites. Mention of trade or firm names does not constitute an endorsement by the USDA. NR 53 TC 3 Z9 3 U1 3 U2 40 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2015 VL 44 IS 3 BP 1011 EP 1022 DI 10.2134/jeq2014.09.0403 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA CG9LP UT WOS:000353637200028 PM 26024281 ER PT J AU Dubey, JP Sykes, JE Shelton, GD Sharp, N Verma, SK Calero-Bernal, R Viviano, J Sundar, N Khan, A Grigg, ME AF Dubey, Jitender P. Sykes, Jane E. Shelton, G. Diane Sharp, Nick Verma, Shiv K. Calero-Bernal, Rafael Viviano, Jenifer Sundar, Natarajan Khan, Asis Grigg, Michael E. TI Sarcocystis caninum and Sarcocystis svanai n. spp. (Apicomplexa: Sarcocystidae) Associated with Severe Myositis and Hepatitis in the Domestic Dog (Canis familiaris) SO JOURNAL OF EUKARYOTIC MICROBIOLOGY LA English DT Article DE Canada; dog; USA ID BEARS URSUS-MARITIMUS; MUSCULAR SARCOCYSTOSIS; NEURONA; ENCEPHALITIS; INFECTION; SCHIZONTS; PARASITE AB There are several reports of Sarcocystis sarcocysts in muscles of dogs, but these species have not been named. Additionally, there are two reports of Sarcocystis neurona in dogs. Here, we propose two new names, Sarcocystis caninum, and Sarcocystis svanai for sarcocysts associated with clinical muscular sarcocystosis in four domestic dogs (Canis familiaris), one each from Montana and Colorado in the USA, and two from British Columbia, Canada. Only the sarcocyst stage was identified. Most of the sarcocysts identified were S. caninum. Sarcocysts were studied using light microscopy, transmission electron microscopy (TEM), and polymerase chain reaction. Based on collective results two new species, S. caninum and S. svanai were designated. Sarcocystis caninum and S. svanai were structurally distinct. Sarcocystis caninum sarcocysts were up to 1.2mm long and up to 75m wide. By light microscopy, the sarcocyst wall was relatively thin and smooth. By TEM, the sarcocyst wall was type 9, 1-2m thick, and contained villar protrusions that lacked microtubules. Bradyzoites in sections were 7-9m long. Sarcocysts of S. svanai were few and were identified by TEM. Sarcocystis svanai sarcocysts were type 1, thin walled (<0.5m), and the wall lacked villar protrusions but had tiny blebs that did not invaginate. DNA was extracted either from infected frozen muscle biopsies or formalin-fixed paraffin-embedded sections. Dogs were either singly infected with S. caninum or multiply co-infected with S. caninum and S. svanai (the result of a mixed infection) based on multilocus DNA sequencing and morphology. BLASTn analysis established that the sarcocysts identified in these dogs were similar to, but not identical to Sarcocystis canis or Sarcocystis arctosi, parasites found to infect polar bears (Ursus maritimus) or brown bears (Ursus arctosi), respectively. However, the S. caninum sequence showed 100% identify over the 18S rRNA region sequenced to that of S. arctica, a parasite known to infect Arctic foxes (Vulpes lagopus). C1 [Dubey, Jitender P.; Verma, Shiv K.; Calero-Bernal, Rafael] ARS, Anim Parasit Dis Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Sykes, Jane E.] Univ Calif Davis, Sch Vet Med, Dept Med & Epidemiol, Davis, CA 95616 USA. [Shelton, G. Diane] Univ Calif San Diego, Dept Pathol, La Jolla, CA 92093 USA. [Sharp, Nick] Canada West Vet Specialists, Vancouver, BC V6R 1E5, Canada. [Viviano, Jenifer] US Army, Joint Pathol Ctr, Vet Pathol Serv, Silver Spring, MD 20910 USA. [Sundar, Natarajan; Khan, Asis; Grigg, Michael E.] NIAID, Mol Parasitol Sect, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. RP Dubey, JP (reprint author), BARC, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov FU NIH; NIAID; Department of Employment and Innovation of the Regional Government of Extremadura, Spain [PO12010]; European Social Fund [PO12010] FX The authors thank Dr. Eric Hoberg for his helpful suggestions concerning taxonomy, and Mr. Efrain Perez, Joint Pathology Center, Veterinary Services, U.S. Army, Silver Spring, Maryland for excellent technical help with electron microscopy. This study was financially support in part by the Intramural Research Program of the NIH and NIAID. M.E.G. is a scholar of the Canadian Institute for Advanced Research Integrated Microbial Biodiversity Program.; R. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura, Spain and the European Social Fund. NR 24 TC 2 Z9 2 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1066-5234 EI 1550-7408 J9 J EUKARYOT MICROBIOL JI J. Eukaryot. Microbiol. PD MAY-JUN PY 2015 VL 62 IS 3 BP 307 EP 317 DI 10.1111/jeu.12182 PG 11 WC Microbiology SC Microbiology GA CG8IQ UT WOS:000353551300004 PM 25256157 ER PT J AU Dubey, JP Ferguson, DJP AF Dubey, Jitender P. Ferguson, David J. P. TI Life Cycle of Hammondia hammondi (Apicomplexa: Sarcocystidae) in Cats SO JOURNAL OF EUKARYOTIC MICROBIOLOGY LA English DT Article DE Gamonts; schizonts; ultrastructure ID TOXOPLASMA-GONDII; DIFFERENTIATION AB Hammondia hammondi and Toxoplasma gondii are feline coccidians that are morphologically, antigenically, and phylogenitically related. Both parasites multiply asexually and sexually in feline intestinal enterocytes, but H. hammondi remains confined to enterocytes whereas T. gondii also parasitizes extra-intestinal tissues of the cat. Here, we studied multiplication of H. hammondi in feline intestine and compared with T. gondii cycle. Five parasite-free cats were inoculated orally with tissue cysts and free bradyzoites from skeletal muscles of gamma interferon gene knockout mice and killed at 1, 3, 4, 6, and 7d later. At 1 and 3d post inoculation (DPI), numerous individual intracellular bradyzoites were detected in histological sections of small intestine. At 4 DPI only schizonts were found and they were located in enterocyte cytoplasm above the host cell nucleus. At 6 and 7 DPI both schizonts and gamonts were seen and they were located in enterocytes. Ultrastucturally, schizogonic and gametogonic development of H. hammondi was similar to T. gondii. However, in H. hammondi merozoites rhoptries were longer, and coiled and contained more micronemes than in T. gondii. Ultrastructural development is illustrated in detail. C1 [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Ferguson, David J. P.] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Lab Sci, Oxford OX3 9DU, England. RP Dubey, JP (reprint author), BARC, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov OI Ferguson, David/0000-0001-5045-819X NR 12 TC 3 Z9 3 U1 4 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1066-5234 EI 1550-7408 J9 J EUKARYOT MICROBIOL JI J. Eukaryot. Microbiol. PD MAY-JUN PY 2015 VL 62 IS 3 BP 346 EP 352 DI 10.1111/jeu.12188 PG 7 WC Microbiology SC Microbiology GA CG8IQ UT WOS:000353551300008 PM 25312612 ER PT J AU Dubey, JP Verma, SK Calero-Bernal, R Cassinelli, AB Kwok, OCH Van Why, K Su, CL Humphreys, JG AF Dubey, Jitender P. Verma, Shiv K. Calero-Bernal, Rafael Cassinelli, Ana B. Kwok, Oliver C. H. Van Why, Kyle Su, Chunlei Humphreys, Jan G. TI Isolation and Genetic Characterization of Toxoplasma gondii from Black Bears (Ursus americanus), Bobcats (Lynx rufus), and Feral Cats (Felis catus) from Pennsylvania SO JOURNAL OF EUKARYOTIC MICROBIOLOGY LA English DT Article DE Genotyping; isolation; seroprevalence ID HIGH PREVALENCE; EPIDEMIOLOGY; ANTIBODIES; WILDLIFE; SEROPREVALENCE; IDENTIFICATION; DISEASE AB Toxoplasma gondii infects virtually all warm-blooded hosts worldwide. Recently, attention has been focused on the genetic diversity of the parasite to explain its pathogenicity in different hosts. It has been hypothesized that interaction between feral and domestic cycles of T. gondii may increase unusual genotypes in domestic cats and facilitate transmission of potentially more pathogenic genotypes to humans, domestic animals, and wildlife. In the present study, we tested black bear (Ursus americanus), bobcat (Lynx rufus), and feral cat (Felis catus) from the state of Pennsylvania for T. gondii infection. Antibodies to T. gondii were found in 32 (84.2%) of 38 bears, both bobcats, and 2 of 3 feral cats tested by the modified agglutination test (cut off titer 1:25). Hearts from seropositive animals were bioassayed in mice, and viable T.gondii was isolated from 3 of 32 bears, 2 of 2 bobcats, and 2 of 3 feral cats. DNA isolated from culture-derived tachyzoites of these isolates was characterized using multilocus PCR-RFLP markers. Three genotypes were revealed, including ToxoDB PCR-RFLP genotype #1 or #3 (Type II, 1 isolate), #5 (Type 12, 3 isolates), and #216 (3 isolates), adding to the evidence of genetic diversity of T. gondii in wildlife in Pennsylvania. Pathogenicity of 3 T. gondii isolates (all #216, 1 from bear, and 2 from feral cat) was determined in outbred Swiss Webster mice; all three were virulent causing 100% mortality. Results indicated that highly mouse pathogenic strains of T. gondii are circulating in wildlife, and these strains may pose risk to infect human through consuming of game meat. C1 [Dubey, Jitender P.; Verma, Shiv K.; Calero-Bernal, Rafael; Cassinelli, Ana B.; Kwok, Oliver C. H.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Van Why, Kyle] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Harrisburg, PA 17106 USA. [Su, Chunlei] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Humphreys, Jan G.] Indiana Univ Penn, Dept Biol, Indiana, PA 15705 USA. RP Dubey, JP (reprint author), BARC, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund [PO12010] FX R. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund. NR 23 TC 1 Z9 1 U1 6 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1066-5234 EI 1550-7408 J9 J EUKARYOT MICROBIOL JI J. Eukaryot. Microbiol. PD MAY-JUN PY 2015 VL 62 IS 3 BP 410 EP 415 DI 10.1111/jeu.12196 PG 6 WC Microbiology SC Microbiology GA CG8IQ UT WOS:000353551300014 PM 25393429 ER PT J AU Knothe, G de Castro, MEG Razon, LF AF Knothe, Gerhard de Castro, Maria Ellenita G. Razon, Luis F. TI Methyl Esters (Biodiesel) from and Fatty Acid Profile of Gliricidia sepium Seed Oil SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Biodiesel; Fatty acid profile; Fuel properties; Gliricidia sepium seed oil; Legume; Methyl esters; Transesterification ID DIESEL FUEL; COMPREHENSIVE EVALUATION; KINEMATIC VISCOSITY; VEGETABLE-OILS; CETANE NUMBERS; FRYING OIL; PETRODIESEL; COMPONENTS; LUBRICITY; BIOFUEL AB Increasing the supply of biodiesel by defining and developing additional feedstocks is important to overcome the still limited amounts available of this alternative fuel. In this connection, the methyl esters of the seed oil of Gliricidia sepium were synthesized and the significant fuel-related properties were determined. The fatty acid profile was also determined with saturated fatty acids comprising slightly more than 35 %, 16.5 % palmitic, 14.5 % stearic, as well as lesser amounts of even longer-chain fatty acids. Linoleic acid is the most prominent acid at about 49 %. Corresponding to the high content of saturated fatty acid methyl esters, cold flow is the most problematic property as shown by a high cloud point of slightly > 20 A degrees C. Otherwise, the properties of G. sepium methyl esters are acceptable for biodiesel use when comparing them to specifications in biodiesel standards but the problematic cold flow properties would need to be observed. The H-1- and C-13-NMR spectra of G. sepium methyl esters are reported. C1 [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [de Castro, Maria Ellenita G.] De La Salle Univ, Dept Biol, Manila, Philippines. [Razon, Luis F.] De La Salle Univ, Dept Chem Engn, Manila, Philippines. RP Knothe, G (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov NR 50 TC 2 Z9 2 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD MAY PY 2015 VL 92 IS 5 BP 769 EP 775 DI 10.1007/s11746-015-2634-3 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CH1XL UT WOS:000353816400016 ER PT J AU Rejeski, WJ Rushing, J Guralnik, JM Ip, EH King, AC Manini, TM Marsh, AP McDermott, MM Fielding, RA Newman, AB Tudor-Locke, C Gill, TM AF Rejeski, W. Jack Rushing, Julia Guralnik, Jack M. Ip, Edward H. King, Abby C. Manini, Todd M. Marsh, Anthony P. McDermott, Mary M. Fielding, Roger A. Newman, Anne B. Tudor-Locke, Catrine Gill, Thomas M. CA LIFE Study Grp TI The MAT-sf: Identifying Risk for Major Mobility Disability SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE MAT-sf; Mobility disability; LIFE study; Geriatric assessment; Physical function ID LIFE-STYLE INTERVENTIONS; SELF-REPORTED DISABILITY; LOWER-EXTREMITY FUNCTION; OLDER-ADULTS; PHYSICAL-ACTIVITY; INDEPENDENCE; PERFORMANCE; MORTALITY; HEALTH; ELDERS AB Background. The assessment of mobility is essential to both aging research and clinical geriatric practice. A newly developed self-report measure of mobility, the mobility assessment tool-short form (MAT-sf), uses video animations as an innovative method to improve measurement accuracy/precision. The primary aim of the current study was to evaluate whether MAT-sf scores can be used to identify risk for major mobility disability (MMD). Methods. This article is based on data collected from the Lifestyle Interventions and Independence for Elders study and involved 1,574 older adults between the ages of 70-89. The MAT-sf was administered at baseline; MMD, operationalized as failure to complete the 400-m walk <= 15 minutes, was evaluated at 6-month intervals across a period of 42 months. The outcome of interest was the first occurrence of MMD or incident MMD. Results. After controlling for age, sex, clinic site, and treatment arm, baseline MAT-sf scores were found to be effective in identifying risk for MMD (p < .0001). Partitioning the MAT-sf into four groups revealed that persons with scores < 40, 40-49, 50-59, and 60+ had failure rates across 42 months of follow-up of 66%, 52%, 35%, and 22%, respectively. Conclusions. The MAT-sf is a quick and efficient way of identifying older adults at risk for MMD. It could be used to clinically identify older adults that are in need of intervention for MMD and provides a simple means for monitoring the status of patients' mobility, an important dimension of functional health. C1 [Rejeski, W. Jack; Marsh, Anthony P.] Wake Forest Univ, Dept Hlth & Exercise Sci, Winston Salem, NC 27109 USA. [Rushing, Julia; Ip, Edward H.] Wake Forest Univ, Sch Med, Div Publ Hlth Sci, Dept Biostat Sci, Winston Salem, NC 27109 USA. [Guralnik, Jack M.] Univ Maryland, Sch Med, Dept Epidemiol & Publ Hlth, Baltimore, MD 21201 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Hlth Res & Policy, Stanford, CA 94305 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [Manini, Todd M.] Univ Florida, Coll Med, Dept Aging & Geriatr Res, Gainesville, FL USA. [McDermott, Mary M.] Northwestern Univ, Dept Internal Med, Chicago, IL 60611 USA. [Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Newman, Anne B.] Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15260 USA. [Tudor-Locke, Catrine] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Gill, Thomas M.] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA. RP Rejeski, WJ (reprint author), Wake Forest Univ, Dept Hlth & Exercise Sci, Box 7868, Winston Salem, NC 27109 USA. EM rejeski@wfu.edu RI Buford, Thomas /B-1111-2011; OI Buford, Thomas /0000-0002-9541-4358; Marsiske, Michael/0000-0001-5973-2116 FU National Institutes of Health/National Institute on Aging [UO1 AG22376]; National Heart, Lung and Blood Institute [3U01AG022376-05A2S]; National Institute on Aging, National Institutes of Health; Claude D. Pepper Older Americans Independence Centers at the University of Florida [1 P30 AG028740]; Wake Forest University [1 P30 AG21332]; Tufts University [1P30AG031679]; University of Pittsburgh [P30 AG024827]; Yale University [P30AG021342]; National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University [UL1 RR025744]; National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at University of Florida [U54RR025208]; National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Yale University [UL1 TR000142]; Boston Rehabilitation Outcomes Center [1R24HD065688-01A1]; National Heart, Lung, and Blood Institute [R18 HL076441]; National Institutes for Aging [P30 AG021332]; General Clinical Research Center [M01-RR007122]; National Institutes on Aging [K07AG043587] FX The Lifestyle Interventions and Independence for Elders study is funded by a National Institutes of Health/National Institute on Aging Cooperative Agreement #UO1 AG22376 and a supplement from the National Heart, Lung and Blood Institute 3U01AG022376-05A2S and sponsored in part by the Intramural Research Program, National Institute on Aging, National Institutes of Health. The research was partially supported by the Claude D. Pepper Older Americans Independence Centers at the University of Florida (1 P30 AG028740), Wake Forest University (1 P30 AG21332), Tufts University (1P30AG031679), University of Pittsburgh (P30 AG024827), and Yale University (P30AG021342) and the National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University (UL1 RR025744), at University of Florida (U54RR025208), and at Yale University (UL1 TR000142). Tufts University is also supported by the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1). W.J.R. was supported, in part, from the following mechanisms: National Heart, Lung, and Blood Institute (R18 HL076441), National Institutes for Aging (P30 AG021332), and General Clinical Research Center (M01-RR007122); T.M.G. is the recipient of an Academic Leadership Award (K07AG043587) from the National Institutes on Aging. NR 24 TC 1 Z9 1 U1 1 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD MAY PY 2015 VL 70 IS 5 BP 641 EP 646 DI 10.1093/gerona/glv003 PG 6 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA CH3AX UT WOS:000353899100014 PM 25680917 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] HHS, NIH, OD, OER,OLAW, Washington, DC 20201 USA. [Gipson, Chester] USDA, APHIS, AC, Washington, DC USA. RP Brown, P (reprint author), HHS, NIH, OD, OER,OLAW, Washington, DC 20201 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD MAY PY 2015 VL 44 IS 5 BP 168 EP 168 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA CH2QK UT WOS:000353869500015 PM 25897933 ER PT J AU Choi, YJ Klosterman, SJ Kummer, V Voglmayr, H Shin, HD Thines, M AF Choi, Young-Joon Klosterman, Steven J. Kummer, Volker Voglmayr, Hermann Shin, Hyeon-Dong Thines, Marco TI Multi-locus tree and species tree approaches toward resolving a complex clade of downy mildews (Straminipila, Oomycota), including pathogens of beet and spinach SO MOLECULAR PHYLOGENETICS AND EVOLUTION LA English DT Article DE Cospeciation; Host shift; Multi-locus phylogeny; Oomycetes; Peronospora farinosa; Species tree ID INTERNAL TRANSCRIBED SPACER; ALBUGO-CANDIDA COMPLEX; PERONOSPORA-FARINOSA; PHYLOGENETIC-RELATIONSHIPS; MOLECULAR PHYLOGENY; RE-CONSIDERATION; RDNA SEQUENCES; DNA-SEQUENCES; CAUSAL AGENT; HOST SHIFT AB Accurate species determination of plant pathogens is a prerequisite for their control and quarantine, and further for assessing their potential threat to crops. The family Peronosporaceae (Straminipila; Oomycota) consists of obligate biotrophic pathogens that cause downy mildew disease on angiosperms, including a large number of cultivated plants. In the largest downy mildew genus Peronospora, a phylogenetically complex clade includes the economically important downy mildew pathogens of spinach and beet, as well as the type species of the genus Peronospora. To resolve this complex clade at the species level and to infer evolutionary relationships among them, we used multi-locus phylogenetic analysis and species tree estimation. Both approaches discriminated all nine currently accepted species and revealed four previously unrecognized lineages, which are specific to a host genus or species. This is in line with a narrow species concept, i.e. that a downy mildew species is associated with only a particular host plant genus or species. Instead of applying the dubious name Peronospora farinosa, which has been proposed for formal rejection, our results provide strong evidence that Peronospora schachtii is an independent species from lineages on Atriplex and apparently occurs exclusively on Beta vulgaris. The members of the clade investigated, the Peronospora rumicis clade, associate with three different host plant families, Amaranthaceae, Caryophyllaceae, and Polygonaceae, suggesting that they may have speciated following at least two recent inter-family host shifts, rather than contemporary cospeciation with the host plants. (C) 2015 Elsevier Inc. All rights reserved. C1 [Choi, Young-Joon; Thines, Marco] Goethe Univ Frankfurt, Fac Biosci, Inst Ecol Evolut & Divers, D-60438 Frankfurt, Germany. [Choi, Young-Joon; Thines, Marco] Biodivers & Climate Res Ctr BiK F, D-60325 Frankfurt, Germany. [Klosterman, Steven J.] USDA ARS, Salinas, CA USA. [Kummer, Volker] Univ Potsdam, Inst Biochem & Biol, D-14469 Potsdam, Germany. [Voglmayr, Hermann] BOKU Univ Nat Resources & Life Sci, Inst Forest Entomol Forest Pathol & Forest Protec, Dept Forest & Soil Sci, A-1190 Vienna, Austria. [Voglmayr, Hermann] Univ Vienna, Dept Bot & Biodivers Res, Div Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Shin, Hyeon-Dong] Korea Univ, Div Environm Sci & Ecol Engn, Seoul 136701, South Korea. [Thines, Marco] Integrat Fungal Res Cluster IPF, D-60325 Frankfurt, Germany. [Thines, Marco] Senckenberg Gesell Nat Forsch, D-60325 Frankfurt, Germany. RP Choi, YJ (reprint author), Biodivers & Climate Res Ctr BiK F, Senckenberganlage 25, D-60325 Frankfurt, Germany. EM mycochoi@gmail.com RI Voglmayr, Hermann/B-1484-2013 OI Voglmayr, Hermann/0000-0001-7666-993X FU Alexander von Humboldt foundation; California Leafy Greens Research Program; Austrian Science Fund (FWF) [P22739-B20]; LOEWE excellence program of the German state of Hessen, in the framework of the Integrative Fungal Research Cluster (IPF); Biodiversity and Climate Research Centre (BiK-F) FX The authors are grateful to curators of BPI (USA), DM (Japan), BRNM (Czech Republic), GLM (Germany), HMAS (China), KUS-F (South Korea), LE (Russia), UPS (Sweden), VPRI (Australia), and WU (Austria) for providing Peronospora specimens. YJC was supported by a fellowship from the Alexander von Humboldt foundation. SJK was supported in part by the California Leafy Greens Research Program. Financial support by the Austrian Science Fund (FWF; project P22739-B20) to HV is gratefully acknowledged. This study was supported by the LOEWE excellence program of the German state of Hessen, in the framework of the Integrative Fungal Research Cluster (IPF) and the Biodiversity and Climate Research Centre (BiK-F). YJC and MT conceived the study with a contribution from SJK. HDS, HV, VK, and YJC provided materials. YJC conducted experiments. YJC analysed and interpreted the data with contributions from MT. YJC and MT wrote the manuscript with contributions from HV, SJK, HDS, VK. NR 108 TC 8 Z9 9 U1 6 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1055-7903 EI 1095-9513 J9 MOL PHYLOGENET EVOL JI Mol. Phylogenet. Evol. PD MAY PY 2015 VL 86 BP 24 EP 34 DI 10.1016/j.ympev.2015.03.003 PG 11 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA CH0XD UT WOS:000353744800003 PM 25772799 ER PT J AU O'Connor, JE Duda, JJ Grant, GE AF O'Connor, J. E. Duda, J. J. Grant, G. E. TI 1000 dams down and counting SO SCIENCE LA English DT Editorial Material ID REMOVAL; RIVER C1 [O'Connor, J. E.] US Geol Survey, Portland, OR 97201 USA. [Duda, J. J.] US Geol Survey, Seattle, WA USA. [Grant, G. E.] US Forest Serv, USDA, Corvallis, OR USA. RP O'Connor, JE (reprint author), US Geol Survey, Portland, OR 97201 USA. EM oconnor@usgs.gov RI Duda, Jeffrey/A-7132-2009 OI Duda, Jeffrey/0000-0001-7431-8634 NR 14 TC 20 Z9 21 U1 16 U2 65 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD MAY 1 PY 2015 VL 348 IS 6234 BP 496 EP 497 DI 10.1126/science.aaa9204 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CH1JR UT WOS:000353778100018 PM 25931537 ER PT J AU Crain, AR Belant, JL DeVault, TL AF Crain, Alexander R. Belant, Jerrold L. DeVault, Travis L. TI Carnivore incidents with US civil aircraft SO TRANSPORTATION RESEARCH PART D-TRANSPORT AND ENVIRONMENT LA English DT Article DE Aircraft; Airport; Carnivore; Wildlife incident; Wildlife management ID WHITE-TAILED DEER; MANAGEMENT; COYOTES; AIRPORTS; STRIKES; HAZARDS; FENCES; CANIS AB Wildlife incidents with aircraft are of concern in the United States as they pose a risk to human safety and economic losses for the aviation industry. Most previous research on wildlife-aircraft incidents has emphasized birds, bats, and ungulates. We queried the Federal Aviation Administration's National Wildlife Strike Database from 1990 to 2012 to characterize carnivore incidents with U.S. civil aircraft. We found 1016 carnivore incidents with aircraft representing at least 16 species, with coyotes (n = 404) being the species most frequently struck. California and Texas had the most reported incidents and incidents were most likely to occur at night from August to November. Overall estimated damage to aircraft was US$ 7 million. Coinciding with the increase in air traffic, the rate of carnivore-aircraft incidents increased 13.1% annually from 1990 to 2012 whereas the rate of damaging incidents remained fairly constant. Due to the increase in carnivore-aircraft incidents from 1990 to 2012, we recommend further research on techniques to increase detection of carnivores and implementation and scheduled maintenance of perimeter high fences for exclusion. Additionally, we recommend increasing patrol of runways, especially during peak incident periods Uuly November) and at night (2000-0600 h). (C) 2015 Elsevier Ltd. All rights reserved. C1 [Crain, Alexander R.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Belant, Jerrold L.] Mississippi State Univ, Forest & Wildlife Res Ctr, Carnivore Ecol Lab, Mississippi State, MS 39762 USA. [DeVault, Travis L.] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. RP Belant, JL (reprint author), Mississippi State Univ, Forest & Wildlife Res Ctr, Carnivore Ecol Lab, Mississippi State, MS 39762 USA. EM ac690@msstate.edu; j.belant@msstate.edu FU Federal Aviation Administration; U.S. Department of Agriculture; Mississippi State University FX We thank the Federal Aviation Administration, U.S. Department of Agriculture, and Mississippi State University for supporting our research. Opinions expressed in this study do not necessarily reflect current FAA policy decisions regarding the control of wildlife on or near airports. NR 38 TC 2 Z9 2 U1 2 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1361-9209 J9 TRANSPORT RES D-TR E JI Transport. Res. Part D-Transport. Environ. PD MAY PY 2015 VL 36 BP 160 EP 166 DI 10.1016/j.trd.2014.12.001 PG 7 WC Environmental Studies; Transportation; Transportation Science & Technology SC Environmental Sciences & Ecology; Transportation GA CH0WF UT WOS:000353742400015 ER PT J AU Lesmeister, DB Nielsen, CK Schauber, EM Hellgren, EC AF Lesmeister, Damon B. Nielsen, Clayton K. Schauber, Eric M. Hellgren, Eric C. TI Spatial and Temporal Structure of a Mesocarnivore Guild in Midwestern North America SO WILDLIFE MONOGRAPHS LA English DT Article DE activity; bobcat (Lynx rufus); carnivore guild structure; central hardwoods; co-occupancy; coyote (Canis latrans); gray fox (Urocyon cinereoargenteus); habitat occupancy; multiscale ecological sorting; raccoon (Procyon lotor); red fox (Vulpes vulpes); striped skunk (Mephitis mephitis) ID FOXES VULPES-VULPES; COARSE WOODY DEBRIS; ESTIMATING SITE OCCUPANCY; SKUNKS MEPHITIS-MEPHITIS; CAUSE-SPECIFIC MORTALITY; EASTERN SPOTTED SKUNKS; MESOPREDATOR RELEASE HYPOTHESIS; YELLOWSTONE-NATIONAL-PARK; SMALL MAMMAL COMMUNITIES; BOBCATS LYNX-RUFUS AB Carnivore guilds play a vital role in ecological communities by cascading trophic effects, energy and nutrient transfer, and stabilizing or destabilizing food webs. Consequently, the structure of carnivore guilds can be critical to ecosystem patterns. Body size is a crucial influence on intraguild interactions, because it affects access to prey resources, effectiveness in scramble competition, and vulnerability to intraguild predation. Coyotes (Canis latrans), bobcats (Lynx rufus), gray foxes (Urocyon cinereoargenteus), raccoons (Procyon lotor), red foxes (Vulpes vulpes), and striped skunks (Mephitis mephitis) occur sympatrically throughout much of North America and overlap in resource use, indicating potential for interspecific interactions. Although much is known about the autecology of the individual species separately, little is known about factors that facilitate coexistence and how interactions within this guild influence distribution, habitat use, and temporal activity of the smaller carnivores. To assess how habitat autecology and interspecific interactions affect the structure of this widespread carnivore guild, we conducted a large-scale, non-invasive carnivore survey using an occupancy modeling framework. We deployed remote cameras during 3-week surveys to detect carnivores at 1,118 camera locations in 357 2.6-km(2) sections (3-4 cameras/section composing a cluster) in the 16 southernmost counties of Illinois (16,058 km(2)) during January-April, 2008-2010. We characterized microhabitat at each camera location and landscape-level habitat features for each camera cluster. In a multistage approach, we used information-theoretic methods to evaluate competing models for detection, species-specific habitat occupancy, multispecies co-occupancy, and multiseason (colonization and extinction) occupancy dynamics. We developed occupancy models for each species to represent hypothesized effects of anthropogenic features, prey availability, landscape complexity, and vegetative land cover. We quantified temporal activity patterns of each carnivore species based on their frequency of appearance in photographs. Further, we assessed whether smaller carnivores shifted their diel activity patterns in response to the presence of potential competitors. Of the 102,711 photographs of endothermic animals, we recorded photographs of bobcats (n=412 photographs), coyotes (n=1,397), gray foxes (n=546), raccoons (n=40,029), red foxes (n=149), and striped skunks (n=2,467). Bobcats were active primarily during crepuscular periods, and their activity was reduced with precipitation and higher temperatures. The probability of detecting bobcats decreased after a bobcat photograph was recorded, suggesting avoidance of remote cameras after the first encounter. Across southern Illinois, bobcat occupancy at the camera-location and camera-cluster scale (local=0.240.04, camera cluster cluster=0.750.06) was negatively influenced by anthropogenic features and infrastructure. Bobcats had high rates of colonization (=0.86) and low rates of extinction (E=0.07), suggesting an expanding population, but agricultural land was less likely to be colonized. Nearly all camera clusters were occupied by coyotes (cluster=0.95 +/- 0.03). At the local scale, coyote occupancy (local=0.58 +/- 0.03) was higher in hardwood forest stands with open understories than in other areas. Compared to coyotes, gray foxes occupied a smaller portion of the study area (local=0.13 +/- 0.01, cluster=0. 29 +/- 0.03) at all scales. At the scale of the camera cluster, gray fox occupancy was highest in fragmented areas with high proportions of forest, and positively related to anthropogenic features within 100% home-range buffers. Red foxes occupied a similar proportion of the study area as gray foxes (local=0.12 +/- 0.02, cluster=0.26 +/- 0.04) but were more closely associated with anthropogenic features. Only anthropogenic feature models made up the 90% confidence set at all scales of analysis for red foxes. Extinction probabilities at the scale of the camera cluster were higher for both gray foxes (E=0.57) and red foxes (E=0.35) than their colonization rates (gray fox =0.16, red fox =0.06), suggesting both species may be declining in southern Illinois. Striped skunks occupied a large portion of the study area (local=0.47 +/- 0.01, cluster=0.79 +/- 0.03) and were associated primarily with anthropogenic features. Raccoons were essentially ubiquitous within the study area, being photographed in 99% of camera clusters. We observed little evidence for spatial partitioning based on interspecific interactions, with the exception of the gray fox-coyote pairs, and found that habitat preferences were more important in structuring the carnivore community. Habitat had a stronger influence on the occupancy of foxes than did the presence of bobcats. However, the level of red fox activity was negatively correlated with bobcat activity. Gray fox occupancy and the number of detections within occupied sites were reduced in camera clusters occupied by coyotes but not bobcat occupancy. Overall, gray fox occupancy was highest at camera locations with fewer hardwood and more conifer trees. However, gray foxes were more likely to occupy camera locations in hardwood stands than conifer stands if coyotes were also present indicating that hardwood stands may enhance gray fox-coyote coexistence. The 2 fox species appeared to co-occur with each other at the local scale more frequently than expected based on their individual selection of habitat. Similarly, occupancy of camera location by red foxes was higher when coyotes were present. These positive spatial associations among canids may be a response to locally high prey abundance or unmeasured habitat variables. Activity levels of raccoons, bobcats, and coyotes were all positively correlated. Overall, our co- occurrence and activity models indicate competitor-driven adjustments in space use among members of a carnivore community might be the exception rather than the norm. Nevertheless, although our results indicate that gray foxes and red foxes currently coexist with bobcats and coyotes, their distribution appears to be contracting on our study area. Coexistence of foxes with larger carnivores may be enhanced by temporal partitioning of activity and by habitat features that reduce vulnerability of intraguild predation. For instance, hardwood stands may contain trees with structure that enhances tree-climbing by gray foxes, a behavior that probably facilitates coexistence with coyotes. Efforts to enhance gray fox populations would likely benefit from increasing the amount of mature oak-hickory forest. Additionally, the varying results from different scales of analyses underscore the importance of considering multiple spatial scales in carnivore community studies. (c) 2015 The Wildlife Society. C1 [Lesmeister, Damon B.; Schauber, Eric M.; Hellgren, Eric C.] So Illinois Univ, Dept Zool, Ctr Ecol, Cooperat Wildlife Res Lab,Life Sci 2 251, Carbondale, IL 62901 USA. [Nielsen, Clayton K.] So Illinois Univ, Dept Forestry, Ctr Ecol, Cooperat Wildlife Res Lab,Life Sci 2 251, Carbondale, IL 62901 USA. RP Lesmeister, DB (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. EM dlesmeister@fs.fed.us OI Hellgren, Eric/0000-0002-3870-472X FU Federal Aid in Wildlife Restoration Project [W-135-R]; Cooperative Wildlife Research Laboratory, Office of the Vice Chancellor for Research, Colleges of Agricultural Sciences and Science; Departments of Zoology and Forestry at Southern Illinois University FX We thank B. Bluett of the Illinois Department of Natural Resources for ensuring we had the funding needed for a large-scale research project via Federal Aid in Wildlife Restoration Project W-135-R. We extend our gratitude to Cooperative Wildlife Research Laboratory, Office of the Vice Chancellor for Research, Colleges of Agricultural Sciences and Science, and Departments of Zoology and Forestry at Southern Illinois University for their support of the project. We thank the field crew (E. Braaten, B. Caldron, B. Easton, C. Gillen, E. Hoffman, C. Holy, H. Kufahl, S. Periquet, S. Ramakrishnan, and L. Wyatt) because this research would not have been possible without their tireless efforts and dedication. We thank the state and federal employees that assisted in gaining approval for surveys on public land. We especially thank the 310 private landowners that allowed access to their property for these surveys. The manuscript is greatly improved based on changes made in response to reviews by G. Feldhamer, J. Goninger, Editor-in-Chief, Associate Editor, and 2 anonymous reviewers. This publication represents the views of the authors, and any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 275 TC 13 Z9 13 U1 36 U2 199 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0084-0173 EI 1938-5455 J9 WILDLIFE MONOGR JI Wildl. Monogr. PD MAY PY 2015 VL 191 IS 1 BP 1 EP 61 DI 10.1002/wmon.1015 PG 61 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CH2ZX UT WOS:000353896300001 ER PT J AU Kelsey, RG Gonzalez-Hernandez, MP Karchesy, J Veluthoor, S AF Kelsey, Rick G. Gonzalez-Hernandez, M. P. Karchesy, Joe Veluthoor, Sheeba TI Volatile terpenoids and tropolones in heartwood extracts of yellow-cedar, Monterey cypress, and their hybrid Leyland cypress SO ANNALS OF FOREST SCIENCE LA English DT Article DE Cupressaceae; Callitropsis nootkatensis; Hesperocyparis macrocarpa; xHesperotropsis leylandii; Monoterpenes; Sesquiterpenes ID NATURAL ORDER CUPRESSALES; AMERICANUM ACARI IXODIDAE; IXODES-SCAPULARIS ACARI; CHAMAECYPARIS-NOOTKATENSIS; ESSENTIAL OIL; PHYTOPHTHORA-RAMORUM; WOOD; ALASKA; NOOTKATONE; CHEMISTRY AB Key message Leyland cypress, an intergeneric hybrid, produces the same volatile heartwood compounds as its parental taxa, yellow-cedar and Monterrey cypress. However, the proportion of total sesquiterpenes and some of the individual components appear unique to their respective heartwoods. Context Leyland cypress, xHesperotropsis leylandii is an intergeneric hybrid between yellow-cedar, Callitropsis nootkatensis, and Monterey cypress, Hesperocyparis macrocarpa. Their heartwoods are protected by bioactive compounds and rated very durable to durable for products used above ground. Several compounds in yellow-cedar and Monterrey cypress heartwoods are also active against various fungi, bacteria, human insect pests, and plant pathogens, whereas Leyland cypress heartwood has never been thoroughly investigated. Aims The first aim for this study was to examine the volatile compounds in ethyl acetate extracts from the heartwood of all three tree species in Oregon. The second aim was to determine the extent Leyland cypress differs from its parental species, and further investigate any of its novel compounds for biological activity. Methods Ethyl acetate extracts of fresh heartwood were prepared for three trees of each species and analyzed by gas chromatography. Results Thirty-three compounds were detected at 0.5 % or greater abundance across all species, and 23 were identified. Carvacrol was the major monoterpene and nootkatin the most abundant tropolone in all three species. Valencene 11, 12-diol and nootkatone topped the list of sesquiterpenes in yellow-cedar and Leyland cypress, respectively, whereas no sesquiterpenes were detected in Monterrey cypress. This appears to be the first report of tropolones hinokitiol, procerin, and nootkatin in Leyland cypress, alpha-thujaplicinol, pygmaein, and procerin in Monterrey cypress, and hinokitiol in yellow-cedar. Conclusions Leyland cypress heartwood does not biosynthesize structurally unique compounds from those produced by its parental species, and is an unlikely source of novel biocides. However, the proportion of total sesquiterpenes and some of the individual components in Leyland cypress heartwood may distinguish it from the heartwood of its parental species. C1 [Kelsey, Rick G.] USDA Forest Serv, PNW Res Stn, Corvallis, OR 97331 USA. [Gonzalez-Hernandez, M. P.] Univ Santiago de Compostela, Dept Crop Prod, Lugo 27002, Spain. [Karchesy, Joe] Oregon State Univ, Wood Sci & Engn, Corvallis, OR 97331 USA. [Veluthoor, Sheeba] CoreValleys Herbal Technol, Calicut 673027, Kerala, India. RP Kelsey, RG (reprint author), USDA Forest Serv, PNW Res Stn, Corvallis, OR 97331 USA. EM rkelsey@fs.fed.us; pilar.gonzalez@usc.es; joe.karchesy@oregonstate.edu; sheeba.veluthoor@gmail.com OI Gonzalez-Hernandez, M.P./0000-0002-0519-1702 FU USDA Forest Service, Pacific Northwest Research Station FX The authors thank the USDA Forest Service, Pacific Northwest Research Station for funds supporting this project. NR 48 TC 1 Z9 1 U1 0 U2 20 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1286-4560 EI 1297-966X J9 ANN FOREST SCI JI Ann. For. Sci. PD MAY PY 2015 VL 72 IS 3 DI 10.1007/s13595-014-0429-6 PG 7 WC Forestry SC Forestry GA CG3ZS UT WOS:000353219900007 ER PT J AU Johnson, TJ Singer, RS Isaacson, RE Danzeisen, JL Lang, K Kobluk, K Rivet, B Borewicz, K Frye, JG Englen, M Anderson, J Davies, PR AF Johnson, Timothy J. Singer, Randall S. Isaacson, Richard E. Danzeisen, Jessica L. Lang, Kevin Kobluk, Kristi Rivet, Bernadette Borewicz, Klaudyna Frye, Jonathan G. Englen, Mark Anderson, Janet Davies, Peter R. TI In Vivo Transmission of an IncA/C Plasmid in Escherichia coli Depends on Tetracycline Concentration, and Acquisition of the Plasmid Results in a Variable Cost of Fitness SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RESISTANT SALMONELLA-ENTERICA; ANTIMICROBIAL RESISTANCE; MULTIDRUG-RESISTANT; UNITED-STATES; FOOD ANIMALS; COMPARATIVE GENOMICS; CONJUGAL TRANSFER; HOST-RANGE; VIRULENCE; GENES AB IncA/C plasmids are broad-host-range plasmids enabling multidrug resistance that have emerged worldwide among bacterial pathogens of humans and animals. Although antibiotic usage is suspected to be a driving force in the emergence of such strains, few studies have examined the impact of different types of antibiotic administration on the selection of plasmid-containing multidrug resistant isolates. In this study, chlortetracycline treatment at different concentrations in pig feed was examined for its impact on selection and dissemination of an IncA/C plasmid introduced orally via a commensal Escherichia coli host. Continuous low-dose administration of chlortetracycline at 50 g per ton had no observable impact on the proportions of IncA/C plasmid- containing E. coli from pig feces over the course of 35 days. In contrast, high-dose administration of chlortetracycline at 350 g per ton significantly increased IncA/C plasmid-containing E. coli in pig feces (P < 0.001) and increased movement of the IncA/C plasmid to other indigenous E. coli hosts. There was no evidence of conjugal transfer of the IncA/C plasmid to bacterial species other than E. coli. In vitro competition assays demonstrated that bacterial host background substantially impacted the cost of IncA/C plasmid carriage in E. coli and Salmonella. In vitro transfer and selection experiments demonstrated that tetracycline at 32 mu g/ml was necessary to enhance IncA/C plasmid conjugative transfer, while subinhibitory concentrations of tetracycline in vitro strongly selected for IncA/C plasmid-containing E. coli. Together, these experiments improve our knowledge on the impact of differing concentrations of tetracycline on the selection of IncA/C-type plasmids. C1 [Johnson, Timothy J.; Singer, Randall S.; Isaacson, Richard E.; Danzeisen, Jessica L.; Lang, Kevin; Kobluk, Kristi; Rivet, Bernadette; Borewicz, Klaudyna; Anderson, Janet] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. [Frye, Jonathan G.; Englen, Mark] USDA ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Athens, GA 30613 USA. [Davies, Peter R.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. RP Johnson, TJ (reprint author), Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. EM joh04207@umn.edu OI Frye, Jonathan/0000-0002-8500-3395 FU National Pork Board [12-080] FX This project was funded by the National Pork Board, project 12-080. Bioinformatics were enabled through support from the Minnesota Supercomputing Institute. NR 48 TC 7 Z9 8 U1 2 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2015 VL 81 IS 10 BP 3561 EP 3570 DI 10.1128/AEM.04193-14 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CG5MV UT WOS:000353336900029 PM 25769824 ER PT J AU Bender, KW Wang, XJ Cheng, GB Kim, HS Zielinski, RE Huber, SC AF Bender, Kyle W. Wang, Xuejun Cheng, George B. Kim, Hyoung Seok Zielinski, Raymond E. Huber, Steven C. TI Glutaredoxin AtGRXC2 catalyses inhibitory glutathionylation of Arabidopsis BRI1-associated receptor-like kinase 1 (BAK1) in vitro SO BIOCHEMICAL JOURNAL LA English DT Article DE brassinosteroid insensitive 1-associated receptor-like kinase 1 (BAK1); glutaredoxin; glutathionylation; receptor-like kinase; redox regulation ID CASTOR-OIL SEEDS; PROTEIN-KINASE; PHOSPHOENOLPYRUVATE CARBOXYLASE; REDOX REGULATION; PHOTOSYNTHETIC ORGANISMS; TYROSINE PHOSPHORYLATION; S-GLUTATHIONYLATION; STRUCTURAL BASIS; ACTIVATION; BRI1 AB Reversible protein phosphorylation, catalysed by protein kinases, is the most widely studied post-translational modification (PTM), whereas the analysis of other modifications such as S-thiolation is in its relative infancy. In a yeast-two-hybrid (Y2H) screen, we identified a number of novel putative brassinosteroid insensitive 1 (BR1)-associated receptor-like kinase 1 (BAK1) interacting proteins including several proteins related to redox regulation. Glutaredoxin (GRX) C2 (AtGRXC2) was among candidate proteins identified in the Y2H screen and its interaction with recombinant Flag-BAK1 cytoplasmic domain was confirmed using an in vitro pull-down approach. We show that BAK1 peptide kinase activity is sensitive to the oxidizing agents H2O2 and diamide in vitro, suggesting that cysteine oxidation might contribute to control of BAK1 activity. Furthermore, BAK1 was glutathionylated and this reaction could occur via a thiolate-dependent reaction with GSSG or a H2O2-dependent reaction with GSH and inhibited kinase activity. Surprisingly, both reactions were catalysed by AtGRXC2 at lower concentrations of GSSG or GSH than reacted non-enzymatically. Using MALDI-TOF MS, we identified Cys(353), Cys(374) and Cys(408) as potential sites of glutathionylation on the BAK1 cytoplasmic domain and directed mutagenesis suggests that Cys(353) and Cys(408) are major sites of GRXC2-mediated glutathionylation. Collectively, these results highlight the potential for redox control of BAK1 and demonstrate the ability of AtGRXC2 to catalyse protein glutathionylation, a function not previously described for any plant GRX. The present work presents a foundation for future studies of glutathionylation of plant receptor-like protein kinases (RLKs) as well as for the analysis of activities of plant GRXs. C1 [Bender, Kyle W.; Wang, Xuejun; Cheng, George B.; Kim, Hyoung Seok; Zielinski, Raymond E.; Huber, Steven C.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Huber, Steven C.] ARS, USDA, Urbana, IL 61801 USA. RP Huber, SC (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM schuber1@illinois.edu OI Bender, Kyle/0000-0002-1805-8097 FU National Science Foundation [IOS1022177]; US Department of Agriculture - Agricultural Research Service; National Center for Research Resources, National Institutes of Health [S10 RR027109 A] FX The present work was supported in part by the National Science Foundation [grant number IOS1022177]; the US Department of Agriculture - Agricultural Research Service; and the National Center for Research Resources, National Institutes of Health [grant number S10 RR027109 A]. NR 54 TC 5 Z9 5 U1 4 U2 29 PU PORTLAND PRESS LTD PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND SN 0264-6021 EI 1470-8728 J9 BIOCHEM J JI Biochem. J. PD MAY 1 PY 2015 VL 467 BP 399 EP 413 DI 10.1042/BJ20141403 PN 3 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CG3YX UT WOS:000353217200004 PM 25678081 ER PT J AU Alkan, Z Duong, FL Hawkes, WC AF Alkan, Zeynep Duong, Frank L. Hawkes, Wayne C. TI Selenoprotein W controls epidermal growth factor receptor surface expression, activation and degradation via receptor ubiquitination SO BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH LA English DT Article DE Selenium; Selenoprotein W; Epidermal growth factor receptor; Ubiquitination; Cancer ID CELL-CYCLE PROGRESSION; EGF RECEPTOR; MEDIATED DEUBIQUITINATION; MULTIPLE MECHANISMS; EPITHELIAL-CELLS; IN-VIVO; PROTEIN; PHOSPHORYLATION; CANCER; IDENTIFICATION AB Epidermal growth factor (EGF) receptor (EGFR) is the founding member of the ErbB family of growth factor receptors that modulate a complex network of intracellular signaling pathways controlling growth, proliferation, differentiation, and motility. Selenoprotein W (SEPW1) is a highly conserved, diet-regulated 9 kDa thioredoxin-like protein required for normal cell cycle progression. We report here that SEPW1 is required for EGF-induced EGFR activation and that it functions by suppressing EGFR ubiquitination and receptor degradation. SEPW1 depletion inhibited EGF-dependent cell cycle entry in breast and prostate epithelial cells. In prostate cells, SEPW1 depletion decreased EGFR auto-phosphorylation, while SEPW1 overexpression increased EGFR autophosphorylation. SEPW1 depletion increased the rate of EGFR degradation, which decreased total and surface EGFR and suppressed EGF-dependent EGFR endocytosis, EGFR dimer formation, and activation of EGF-dependent pathways. EGFR ubiquitination was increased in SEPW1-depleted cells - in agreement with the increased rate of EGFR degradation, and suggests that SEPW1 suppresses EGFR ubiquitination. Ubiquitination-directed lysozomal degradation controls post-translational EGFR expression and is dysregulated in many cancers. Thus, suppression of EGFR ubiquitination by SEPW1 may be related to the putative increase in cancer risk associated with high selenium intakes. Knowledge of the mechanisms underlying SEPW1's regulation of EGFR ubiquitination may reveal new opportunities for nutritional cancer prevention or cancer drug development. Published by Elsevier B.V. C1 [Alkan, Zeynep; Hawkes, Wayne C.] Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Duong, Frank L.] Cedars Sinai Med Ctr, Dept Med, West Hollywood, CA 90048 USA. RP Alkan, Z (reprint author), Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM zeynep.alkan@ars.usda.gov FU United States Department of Agriculture CRIS [5306-51530-018-00D] FX This work was supported by the United States Department of Agriculture CRIS Project 5306-51530-018-00D. NR 45 TC 3 Z9 3 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4889 EI 0006-3002 J9 BBA-MOL CELL RES JI Biochim. Biophys. Acta-Mol. Cell Res. PD MAY PY 2015 VL 1853 IS 5 BP 1087 EP 1095 DI 10.1016/j.bbamcr.2015.02.016 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA CG3JO UT WOS:000353176700019 PM 25721765 ER PT J AU Keitt, B Griffiths, R Boudjelas, S Broome, K Cranwell, S Millett, J Pitt, W Samaniego-Herrera, A AF Keitt, B. Griffiths, R. Boudjelas, S. Broome, K. Cranwell, S. Millett, J. Pitt, W. Samaniego-Herrera, A. TI Best practice guidelines for rat eradication on tropical islands SO BIOLOGICAL CONSERVATION LA English DT Article DE Best practice; Rat eradication; Tropical islands; Aerial broadcast; Restoration ID NEW-ZEALAND ISLANDS; INTRODUCED RATS; BAIT; BRODIFACOUM; BROADCAST; RODENTS AB Invasive vertebrates are a leading cause of extinction on islands and rats (Rattus spp.) are one of the most damaging to island ecosystems. Methods to eradicate rats from islands are well established and there have been over 580 successful eradications to date. Increasingly, rat eradications are being implemented on tropical islands, a reflection of the need to protect the threatened biodiversity in the tropics. Yet rat eradications on tropical islands fail more frequently than those in temperate climates. In an effort to identify the main reasons for the lower success rate on tropical islands and possible solutions, a workshop was convened with 34 experts in rat eradication, tropical rodent and island ecology and toxicology. The workshop focused on projects using aerial broadcast of brodifacoum, a 2nd generation anticoagulant, because this approach has provided the highest success rate for eradicating rodents from islands. The workshop participants reviewed previously identified challenges to successful rat eradications on tropical islands including increased insect and crab densities resulting in competition for bait, year round or unpredictable timing of breeding rats and increased or unpredictable availability of alternative, natural foods. They also identified a number of new, likely reasons for the lower success rate on tropical islands and provided recommendations for how to address these risks in the planning and implementation of rat eradications. While the success rate of aerial broadcast rat eradications in tropical environments is quite high at 89% (n = 47), it is hoped that by following the recommended best practices provided in this paper, future success rates will be closer to the 96.5% (n = 116) demonstrated for aerial broadcast rat eradications on temperate islands. (C) 2014 The Authors. Published by Elsevier Ltd. C1 [Keitt, B.; Griffiths, R.] Isl Conservat, Santa Cruz, CA 95060 USA. [Boudjelas, S.] Univ Auckland, Sch Biol Sci, Pacific Invas Initiat, Auckland 1142, New Zealand. [Broome, K.] Dept Conservat, Hamilton, New Zealand. [Cranwell, S.] Birdlife Int, Suva, Fiji. [Millett, J.] Royal Soc Protect Birds, Edinburgh, Midlothian, Scotland. [Pitt, W.] USDA APHIS, Natl Wildlife Res Ctr, Wildlife Serv, Hawaii Field Stn, Hilo, HI USA. [Samaniego-Herrera, A.] Grp Ecol & Conservac Islas, Ensenada, Baja California, Mexico. [Samaniego-Herrera, A.] Univ Auckland, Sch Biol Sci, Auckland 1142, New Zealand. RP Keitt, B (reprint author), Isl Conservat, 2161 Delaware Ave,Suite A, Santa Cruz, CA 95060 USA. EM Brad.Keitt@islandconservation.org NR 49 TC 13 Z9 13 U1 7 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD MAY PY 2015 VL 185 SI SI BP 17 EP 26 DI 10.1016/j.biocon.2014.10.014 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CG2FH UT WOS:000353089800003 ER PT J AU Pott, M Wegmann, AS Griffiths, R Samaniego-Herrera, A Cuthbert, RJ Brooke, MD Pitt, WC Berentsen, AR Holmes, ND Howald, GR Ramos-Rendon, K Russell, JC AF Pott, Madeleine Wegmann, Alexander S. Griffiths, Richard Samaniego-Herrera, Araceli Cuthbert, Richard J. Brooke, M. de L. Pitt, William C. Berentsen, Are R. Holmes, Nick D. Howald, Gregg R. Ramos-Rendon, Karina Russell, James C. TI Improving the odds: Assessing bait availability before rodent eradications to aid in selecting bait application rates SO BIOLOGICAL CONSERVATION LA English DT Article DE Rodent; Island; Conservation; Crab; Tropical; Eradication ID LAND CRABS; ISLANDS; ACCEPTANCE AB Rodent eradications undertaken on tropical islands are more likely to fail than eradications undertaken at higher latitudes. We report on 12 independent rodent eradication projects undertaken on tropical islands that utilized the results of an in situ bait availability study prior to eradication to inform, a priori, the bait application rate selected for the eradication. These projects also monitored bait availability during the eradication. The results from our analysis verified the utility of bait availability studies to future rodent eradication campaigns and confirmed the influence of two environmental factors that can affect bait availability over time: precipitation prior to the study and the abundance of land crabs at the study site. Our findings should encourage eradication teams to conduct in-depth assessments of the targeted island prior to project implementation. However, we acknowledge the limitations of such studies (two of the projects we reviewed failed and one removed only one of two rodent species present) and provide guidance on how to interpret the results from a bait availability study in planning an eradication. Study design was inconsistent among the twelve cases we reviewed which limited our analysis. We recommend a more standardized approach for measuring bait availability prior to eradication to provide more robust predictions of the rate at which bait availability will decrease during the eradication and to facilitate future comparisons among projects and islands. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Pott, Madeleine; Wegmann, Alexander S.; Griffiths, Richard; Holmes, Nick D.; Howald, Gregg R.] Isl Conservat, Santa Cruz, CA 95060 USA. [Samaniego-Herrera, Araceli; Ramos-Rendon, Karina] Grp Ecol & Conservac Islas, Ensenada, Baja California, Mexico. [Samaniego-Herrera, Araceli; Russell, James C.] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand. [Samaniego-Herrera, Araceli; Russell, James C.] Univ Auckland, Dept Stat, Auckland 1, New Zealand. [Cuthbert, Richard J.] Royal Soc Protect Birds, Sandy SG19 2DL, Beds, England. [Brooke, M. de L.] Univ Cambridge, Dept Zool, Cambridge CB2 3EJ, England. [Pitt, William C.] USDA APHIS WS, Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96721 USA. [Berentsen, Are R.] USDA APHIS WS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Pott, M (reprint author), Isl Conservat, 2161 Delaware Ave,Suite A, Santa Cruz, CA 95060 USA. EM madeleine.pott@islandconservation.org FU David and Lucile Packard Foundation; Marisla Foundation; Fundacion Carlos Slim - WWF Mexico; USFWS; NFWF; CONACYT; WWF; UK Government; UK Overseas Territories Environment Programme; David & Lucile Packard Foundation FX Thanks to GECI, IC, RSPB, and USDA for making the bait availability data available for analysis. Thanks also to all landowners and partners who made these projects possible, including: U.S. Fish & Wildlife Service (USFWS), U.S. Air Force, The Nature Conservancy, Parque Nacional Galapagos, Conservation Society of Pohnpei and the Municipality of Madolenihmw, and the Commonwealth of the Bahamas. We have The Nature Conservancy, Charles Darwin Foundation, INIFAP (Mexico), and the NCDC of NOAA to thank for collecting and making climatological data available. Island Conservation and its partners are grateful for the years of philanthropic support provided by its many donors and funders. The list of those who provided support is too long to present here, but all are acknowledged in our annual and impact reports, dating back to 2004-2005, which can be found at: http://www.islandconservation.org/about/?id=37. All projects on Mexican islands (Cayo Norte Mayor, Isabel, Muertos, Pajaros and Perez) were planned and implemented by Grupo de Ecologia y Conservacion de Islas (GECI) and mainly funded by The David and Lucile Packard Foundation, Marisla Foundation, Fundacion Carlos Slim - WWF Mexico, USFWS and NFWF, with valuable in kind support from the Mexican Government (SEMAR, SEGOB, SEMARNAT, CONANP, CONABIO and INECC). A.S.-H. thanks CONACYT for the doctoral scholarship and WWF for the EFN fellowship. The Henderson Project was supported by the UK Government, the UK Overseas Territories Environment Programme and the David & Lucile Packard Foundation, administered by the RSPB through the untiring dedication of Kathy Berkery, Geoff Hilton and Juliet Vickery. The Pitcairn community generously supported the conservation work, as did the crews of MVs Braveheart, Claymore and Aquila. The work on Henderson would not have been possible without the generous help of private donors, especially Susan Orr. NR 45 TC 9 Z9 9 U1 5 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD MAY PY 2015 VL 185 SI SI BP 27 EP 35 DI 10.1016/j.biocon.2014.09.049 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CG2FH UT WOS:000353089800004 ER PT J AU Pitt, WC Berentsen, AR Shiels, AB Volker, SF Eisemann, JD Wegmann, AS Howald, GR AF Pitt, William C. Berentsen, Are R. Shiels, Aaron B. Volker, Steven F. Eisemann, John D. Wegmann, Alexander S. Howald, Gregg R. TI Non-target species mortality and the measurement of brodifacoum rodenticide residues after a rat (Rattus rattus) eradication on Palmyra Atoll, tropical Pacific SO BIOLOGICAL CONSERVATION LA English DT Article DE Aerial broadcast; Brodifacoum rodenticide; Invasive rodent eradication; Toxicant residue; Trophic level bioaccumulation; Tropical island forest ID AERIAL POISONING OPERATION; NEW-ZEALAND; INVASIVE RODENTS; BLACK RATS; ISLAND; INVERTEBRATES; IMPACTS; BIRDS; BAITS; ENVIRONMENT AB The use of rodenticides to control or eradicate invasive rats (Rattus spp.) for conservation purposes has rapidly grown in the past decades, especially on islands. The non-target consequences and the fate of toxicant residue from such rodent eradication operations have not been well explored. In a cooperative effort, we monitored the application of a rodenticide, 'Brodifacoum 25W: Conservation', during an attempt to eradicate Rattus rattus from Palmyra Atoll. In 2011, Brodifacoum 25W: Conservation was aerially broadcasted twice over the entire atoll (2.5 km(2)) at rates of 80 kg/ha and 75 kg/ha and a supplemental hand broadcast application (71.6 kg/ha) occurred three weeks after the second aerial application over a 10 ha area. We documented brodifacoum residues in soil, water, and biota, and documented mortality of non-target organisms. Some bait (14-19% of the target application rate) entered the marine environment to distances 7 m from the shore. After the application commenced, carcasses of 84 animals representing 15 species of birds, fish, reptiles and invertebrates were collected opportunistically as potential non-target mortalities. In addition, fish, reptiles, and invertebrates were systematically collected for residue analysis. Brodifacoum residues were detected in most (84.3%) of the animal samples analyzed. Although detection of residues in samples was anticipated, the extent and concentrations in many parts of the food web were greater than expected. Risk assessments should carefully consider application rates and entire food webs prior to operations using rodenticides. Published by Elsevier Ltd. C1 [Pitt, William C.; Shiels, Aaron B.] USDA APHIS, Wildlife Serv, Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96720 USA. [Berentsen, Are R.; Volker, Steven F.; Eisemann, John D.] USDA APHIS, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Wegmann, Alexander S.] Hawaii Program Off, Isl Conservat, Honolulu, HI 96850 USA. [Howald, Gregg R.] Isl Conservat, Vancouver, BC V6B 1H5, Canada. RP Pitt, WC (reprint author), Smithsonian Conservat Biol Inst, Natl Zool Pk,1500 Remount Rd, Front Royal, VA 22630 USA. EM PittW@SI.edu; Are.R.Berentsen@aphis.usda.gov; Aaron.B.Shiels@aphis.usda.gov; Steven.F.Volker@aphis.usda.gov; John.D.Eisemann@aphis.usda.gov; Alex.Wegmann@islandconservation.org; Gregg.Howald@islandconservation.org FU IC; USFWS; TNC; USDA FX The authors wish to thank T. McAuliffe (USDA), K. Hayes (USDA), A. Meyer (USFWS), S. Hathaway (USGS) and A. Kristof (USFWS) for exceptional assistance in the field. We thank D. Griffin and D. Goldade for assistance with residue analysis and B. Kimball for editorial suggestions. This study was funded by IC, USFWS, TNC, and USDA. Author contributions: WCP and ARB conceptualized the project, collected, analyzed, and interpreted data, and wrote the paper; ABS wrote the paper; SFV collected data; JDE conceptualized the project. ASW and GRH assisted in the project design. NR 54 TC 9 Z9 9 U1 10 U2 39 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD MAY PY 2015 VL 185 SI SI BP 36 EP 46 DI 10.1016/j.biocon.2015.01.008 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CG2FH UT WOS:000353089800005 ER PT J AU Sang, J Allen, P Dunbar, J Hanson, G AF Sang, Joseph Allen, Peter Dunbar, John Hanson, Gregory TI Development of semi-physically based model to predict erosion rate of kaolinite clay under different moisture content SO CANADIAN GEOTECHNICAL JOURNAL LA English DT Article DE concentrated flow erosion; semi-physically based model; tensile strength; moisture content; cohesive soils; kaolinite clay ID CRITICAL SHEAR-STRESS; TENSILE-STRENGTH; COHESIVE SOILS; SURFACE ERODIBILITY; COMPACTED CLAYS; WATER EROSION; GULLY EROSION; OVERLAND-FLOW; SCOUR; RESISTANCE AB Understanding the susceptibility of soils to concentrated flow erosion is imperative for predicting sustainability of various engineering structures and assessing environmental integrity. Currently, a widely used model is empirical in nature. In this study, we developed a semi-physically based model that predicts the rate of concentrated flow erosion of kaolinite clay based on tensile and erodibility characteristics. To develop this model, direct tensile tests and jet erosion tests (JETs) were performed on kaolinite clay with different percent moisture contents (MCs). The direct tensile test results showed that the energy required to break interparticle bonds across a fracture plane and tensile strength decreases with an increase in MC, whereas the JET results showed that soil resistance to erosion decreases with an increase in MC. Results also showed that an efficiency index of the JET apparatus, which represents the fraction of jet power used in actual erosion processes, diminishes with a decrease in MC. This semi-physically based model predicted the rate of erosion of kaolinite clay for a range of MC and applied hydraulic shear stress. In model development and verification, 98% and 90% of the data, respectively, were within a discrepancy ratio of 0.50 and 2.0. C1 [Sang, Joseph; Allen, Peter; Dunbar, John] Baylor Univ, Dept Geol, Waco, TX 76798 USA. [Hanson, Gregory] USDA ARS, HERU, Stillwater, OK 74075 USA. RP Sang, J (reprint author), Baylor Univ, Dept Geol, One Bear Pl 97354, Waco, TX 76798 USA. EM Joseph_Sang@Baylor.edu NR 81 TC 1 Z9 1 U1 3 U2 12 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0008-3674 EI 1208-6010 J9 CAN GEOTECH J JI Can. Geotech. J. PD MAY PY 2015 VL 52 IS 5 BP 577 EP 586 DI 10.1139/cgj-2012-0274 PG 10 WC Engineering, Geological; Geosciences, Multidisciplinary SC Engineering; Geology GA CG8TI UT WOS:000353585000004 ER PT J AU Jorgenson, MT Marcot, BG Swanson, DK Jorgenson, JC DeGange, AR AF Jorgenson, Mark Torre Marcot, Bruce G. Swanson, David K. Jorgenson, Janet C. DeGange, Anthony R. TI Projected changes in diverse ecosystems from climate warming and biophysical drivers in northwest Alaska SO CLIMATIC CHANGE LA English DT Article ID PERMAFROST DEGRADATION; NORTHERN ALASKA; VEGETATION; FEEDBACKS; AMERICA; GLACIER; SHIFTS; FIRE; USA AB Climate warming affects arctic and boreal ecosystems by interacting with numerous biophysical factors across heterogeneous landscapes. To assess potential effects of warming on diverse local-scale ecosystems (ecotypes) across northwest Alaska, we compiled data on historical areal changes over the last 25-50 years. Based on historical rates of change relative to time and temperature, we developed three state-transition models to project future changes in area for 60 ecotypes involving 243 potential transitions during three 30-year periods (ending 2040, 2070, 2100). The time model, assuming changes over the past 30 years continue at the same rate, projected a net change, or directional shift, of 6 % by 2100. The temperature model, using past rates of change relative to the past increase in regional mean annual air temperatures (1 A degrees C/30 year), projected a net change of 17 % in response to expected warming of 2, 4, and 6 A degrees C at the end of the three periods. A rate-adjusted temperature model, which adjusted transition rates (+/- 50 %) based on assigned feedbacks associated with 23 biophysical drivers, estimated a net change of 13 %, with 33 ecotypes gaining and 23 ecotypes losing area. Major drivers included shrub and tree expansion, fire, succession, and thermokarst. Overall, projected changes will be modest over the next century even though climate warming increased transition rates up to 9 fold. The strength of this state-transition modeling is that it used a large dataset of past changes to provide a comprehensive assessment of likely future changes associated with numerous drivers affecting the full diversity of ecosystems across a broad region. C1 [Jorgenson, Mark Torre] Alaska Ecosci, Fairbanks, AK 99709 USA. [Marcot, Bruce G.] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97205 USA. [Swanson, David K.] Natl Pk Serv, Fairbanks, AK 99709 USA. [Jorgenson, Janet C.] Arctic Natl Wildlife Refuge, Fairbanks, AK 99701 USA. [DeGange, Anthony R.] US Geol Survey, Alaska Climate Sci Ctr, Anchorage, AK 99508 USA. RP Jorgenson, MT (reprint author), Alaska Ecosci, 2332 Cordes Way, Fairbanks, AK 99709 USA. EM ecoscience@alaska.net FU U.S. Geological Survey FX This project was funded by the U.S. Geological Survey, with in-kind support from the National Park Service, Fish and Wildlife Service, and USDA Forest Serve. We appreciate the input of Robert Winfree, Carl Roland, Leslie Holland-Bartels, Carl Markon, Terry Chapin, and one anonymous reviewer. NR 31 TC 7 Z9 7 U1 7 U2 42 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD MAY PY 2015 VL 130 IS 2 BP 131 EP 144 DI 10.1007/s10584-014-1302-1 PG 14 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CG7EL UT WOS:000353465100006 ER PT J AU Marcot, BG Jorgenson, MT Lawler, JP Handel, CM DeGange, AR AF Marcot, Bruce G. Jorgenson, M. Torre Lawler, James P. Handel, Colleen M. DeGange, Anthony R. TI Projected changes in wildlife habitats in Arctic natural areas of northwest Alaska SO CLIMATIC CHANGE LA English DT Article ID SHRUB EXPANSION; CLIMATE-CHANGE; TUNDRA ECOSYSTEMS; HERBIVORES; CARIBOU; POPULATIONS; RESPONSES; DYNAMICS AB We project the effects of transitional changes among 60 vegetation and other land cover types ("ecotypes") in northwest Alaska over the 21st century on habitats of 162 bird and 39 mammal species known or expected to occur regularly in the region. This analysis, encompassing a broad suite of arctic and boreal wildlife species, entailed building wildlife-habitat matrices denoting levels of use of each ecotype by each species, and projecting habitat changes under historic and expected accelerated future rates of change from increasing mean annual air temperature based on the average of 5 global climate models under the A1B emissions scenario, and from potential influence of a set of 23 biophysical drivers. Under historic rates of change, we project that 52 % of the 201 species will experience an increase in medium- and high-use habitats, 3 % no change, and 45 % a decrease, and that a greater proportion of mammal species (62 %) will experience habitat declines than will bird species (50 %). Outcomes become more dire (more species showing habitat loss) under projections made from effects of biophysical drivers and especially from increasing temperature, although species generally associated with increasing shrub and tree ecotypes will likely increase in distribution. Changes in wildlife habitats likely will also affect trophic cascades, ecosystem function, and ecosystem services; of particular significance are the projected declines in habitats of most small mammals that form the prey base for mesocarnivores and raptors, and habitat declines in 25 of the 50 bird and mammal species used for subsistence hunting and trapping. C1 [Marcot, Bruce G.] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97204 USA. [Jorgenson, M. Torre] Alaska Ecosci, Fairbanks, AK USA. [Lawler, James P.] Natl Pk Serv, Fairbanks, AK USA. [Handel, Colleen M.; DeGange, Anthony R.] US Geol Survey, Anchorage, AK USA. RP Marcot, BG (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97204 USA. EM bmarcot@fs.fed.us OI Handel, Colleen/0000-0002-0267-7408 FU Natural Resources Preservation Program (NRPP) of U.S. Geological Survey's Ecosystems Mission Area FX We thank Leslie Holland-Bartels, Carl Markon, and Robert Winfree for their discussions and administrative guidance on the project. The bird- and mammal-ecotype associations were reviewed by Andrew Hope, David Gustine, and Dan Ruthrauff, and bird subsistence species were identified by Alex Whiting. We thank Andrew Hope, Robert Winfree, Knut Kielland, five anonymous reviewers, and an editor for comments on the manuscript. This project was funded through the Natural Resources Preservation Program (NRPP) of U.S. Geological Survey's Ecosystems Mission Area, and support in kind was provided by U.S. Forest Service, Pacific Northwest Research Station. Any use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 34 TC 4 Z9 4 U1 12 U2 72 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD MAY PY 2015 VL 130 IS 2 BP 145 EP 154 DI 10.1007/s10584-015-1354-x PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CG7EL UT WOS:000353465100007 ER PT J AU Shanley, CS Pyare, S Goldstein, MI Alaback, PB Albert, DM Beier, CM Brinkman, TJ Edwards, RT Hood, E MacKinnon, A McPhee, MV Patterson, TM Suring, LH Tallmon, DA Wipfli, MS AF Shanley, Colin S. Pyare, Sanjay Goldstein, Michael I. Alaback, Paul B. Albert, David M. Beier, Colin M. Brinkman, Todd J. Edwards, Rick T. Hood, Eran MacKinnon, Andy McPhee, Megan V. Patterson, Trista M. Suring, Lowell H. Tallmon, David A. Wipfli, Mark S. TI Climate change implications in the northern coastal temperate rainforest of North America SO CLIMATIC CHANGE LA English DT Article ID SEA-LEVEL RISE; DISSOLVED ORGANIC-MATTER; BLACK-TAILED DEER; SOUTHEAST ALASKA; ECOSYSTEM SERVICES; YELLOW-CEDAR; SPECIES DISTRIBUTION; PACIFIC-NORTHWEST; SITKA SPRUCE; SALMON AB We synthesized an expert review of climate change implications for hydroecological and terrestrial ecological systems in the northern coastal temperate rainforest of North America. Our synthesis is based on an analysis of projected temperature, precipitation, and snowfall stratified by eight biogeoclimatic provinces and three vegetation zones. Five IPCC CMIP5 global climate models (GCMs) and two representative concentration pathways (RCPs) are the basis for projections of mean annual temperature increasing from a current average (1961-1990) of 3.2 A degrees C to 4.9-6.9 A degrees C (5 GCM range; RCP4.5 scenario) or 6.4-8.7 A degrees C (RCP8.5), mean annual precipitation increasing from 3130 mm to 3210-3400 mm (3-9 % increase) or 3320-3690 mm (6-18 % increase), and total precipitation as snow decreasing from 1200 mm to 940-720 mm (22-40 % decrease) or 720-500 mm (40-58 % decrease) by the 2080s (2071-2100; 30-year normal period). These projected changes are anticipated to result in a cascade of ecosystem-level effects including: increased frequency of flooding and rain-on-snow events; an elevated snowline and reduced snowpack; changes in the timing and magnitude of stream flow, freshwater thermal regimes, and riverine nutrient exports; shrinking alpine habitats; altitudinal and latitudinal expansion of lowland and subalpine forest types; shifts in suitable habitat boundaries for vegetation and wildlife communities; adverse effects on species with rare ecological niches or limited dispersibility; and shifts in anadromous salmon distribution and productivity. Our collaborative synthesis of potential impacts highlights the coupling of social and ecological systems that characterize the region as well as a number of major information gaps to help guide assessments of future conditions and adaptive capacity. C1 [Shanley, Colin S.; Albert, David M.] Nature Conservancy, Juneau, AK 99801 USA. [Pyare, Sanjay; Hood, Eran] Univ Alaska Southeast, Environm Sci & Geog Program, Juneau, AK 99801 USA. [Goldstein, Michael I.] US Forest Serv, Juneau, AK 99801 USA. [Alaback, Paul B.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. [Beier, Colin M.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Brinkman, Todd J.] Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, Fairbanks, AK 99709 USA. [Edwards, Rick T.] US Forest Serv, Pacific NW Res Stn, Juneau, AK 99801 USA. [MacKinnon, Andy] British Columbia Forest Serv, Victoria, BC, Canada. [McPhee, Megan V.] Univ Alaska Fairbanks, Div Fisheries, Juneau, AK 99801 USA. [Patterson, Trista M.] US Forest Serv, Pacific NW Res Stn, Sitka, AK 99835 USA. [Suring, Lowell H.] Northern Ecol LLC, Suring, WI 54174 USA. [Tallmon, David A.] Univ Alaska Southeast, Biol & Marine Biol Program, Juneau, AK 99801 USA. [Wipfli, Mark S.] Univ Alaska Fairbanks, Inst Arctic Biol, Alaska Cooperat Fish & Wildlife Res Unit, US Geol Survey, Fairbanks, AK 99775 USA. RP Shanley, CS (reprint author), Nature Conservancy, 416 Harris St,Suite 301, Juneau, AK 99801 USA. EM cshanley@tnc.org FU Wilburforce Foundation; Gordon and Betty Moore Foundation; North Pacific Landscape Conservation Cooperative; NSF award [OIA-1208927]; state of Alaska FX We would like to thank the Wilburforce Foundation and the Gordon and Betty Moore Foundation for funding C.S. Shanley to conduct the climate modeling and manuscript drafting. Wilburforce, along with the North Pacific Landscape Conservation Cooperative, provided support to M. I. Goldstein and S. Pyare for stimulating cross-boundary collaboration. Alaska EPSCoR NSF award #OIA-1208927 and the state of Alaska supported S. Pyare during the drafting of the manuscript. NR 92 TC 3 Z9 3 U1 11 U2 79 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD MAY PY 2015 VL 130 IS 2 BP 155 EP 170 DI 10.1007/s10584-015-1355-9 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CG7EL UT WOS:000353465100008 ER PT J AU Raghavan, S Porneala, B McKeown, N Fox, CS Dupuis, J Meigs, JB AF Raghavan, Sridharan Porneala, Bianca McKeown, Nicola Fox, Caroline S. Dupuis, Josee Meigs, James B. TI Metabolic factors and genetic risk mediate familial type 2 diabetes risk in the Framingham Heart Study SO DIABETOLOGIA LA English DT Article DE Diet; Family history; Genetic risk; Metabolic syndrome; Physical activity; Type 2 diabetes ID INSULIN-SECRETION; LIFE-STYLE; GLUCOSE-TOLERANCE; PHYSICAL-ACTIVITY; YOUNG ADULTHOOD; GLYCEMIC TRAITS; MELLITUS; ASSOCIATION; PARENTS; HERITABILITY AB Aims/hypothesis Type 2 diabetes mellitus in parents is a strong determinant of diabetes risk in their offspring. We hypothesise that offspring diabetes risk associated with parental diabetes is mediated by metabolic risk factors. Methods We studied initially non-diabetic participants of the Framingham Offspring Study. Metabolic risk was estimated using beta cell corrected insulin response (CIR), HOMA-IR or a count of metabolic syndrome components (metabolic syndrome score [MSS]). Dietary risk and physical activity were estimated using questionnaire responses. Genetic risk score (GRS) was estimated as the count of 62 type 2 diabetes risk alleles. The outcome of incident diabetes in offspring was examined across levels of parental diabetes exposure, accounting for sibling correlation and adjusting for age, sex and putative mediators. The proportion mediated was estimated by comparing regression coefficients for parental diabetes with (beta (adj)) and without (beta (unadj)) adjustments for CIR, HOMA-IR, MSS and GRS (percentage mediated = 1 - beta(adj)/beta(unadj)). Results Metabolic factors mediated 11% of offspring diabetes risk associated with parental diabetes, corresponding to a reduction in OR per diabetic parent from 2.13 to 1.96. GRS mediated 9% of risk, corresponding to a reduction in OR per diabetic parent from 2.13 to 1.99. Conclusions/interpretation Metabolic risk factors partially mediated offspring type 2 diabetes risk conferred by parental diabetes to a similar magnitude as genetic risk. However, a substantial proportion of offspring diabetes risk associated with parental diabetes remains unexplained by metabolic factors, genetic risk, diet and physical activity, suggesting that important familial influences on diabetes risk remain undiscovered. C1 [Raghavan, Sridharan; Porneala, Bianca; Meigs, James B.] Harvard Univ, Sch Med, Dept Med, Div Gen Internal Med, Boston, MA 02114 USA. [Raghavan, Sridharan; Porneala, Bianca; Meigs, James B.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [McKeown, Nicola] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Fox, Caroline S.] NHLBI, Lab Metab & Populat Hlth, Framingham, MA USA. [Dupuis, Josee] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. RP Meigs, JB (reprint author), Harvard Univ, Sch Med, Dept Med, Div Gen Internal Med, 50 Staniford St,9th Floor, Boston, MA 02114 USA. EM jmeigs@partners.org FU National Institutes of Health National Research Service [T32HP10251]; Ryoichi Sasakawa Fellowship Fund; NIH [RO1DK78616]; National Heart, Lung and Blood Institute's Framingham Heart Study [N01-HC-25195]; Affymetrix for genotyping services [N02-HL-6-4278]; USDA [1950-51530-011-00D]; [K24 DK080140] FX SR is supported by National Institutes of Health National Research Service Award T32HP10251 and the Ryoichi Sasakawa Fellowship Fund. JBM and JD are supported by NIH RO1DK78616, and JBM is also supported by K24 DK080140. We gratefully acknowledge the FHS study participants and staff for their contributions. This work was partially supported by the National Heart, Lung and Blood Institute's Framingham Heart Study (Contract No. N01-HC-25195) and its contract with Affymetrix for genotyping services (Contract No. N02-HL-6-4278). NM is supported by USDA agreement number 1950-51530-011-00D. NR 32 TC 1 Z9 1 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0012-186X EI 1432-0428 J9 DIABETOLOGIA JI Diabetologia PD MAY PY 2015 VL 58 IS 5 BP 988 EP 996 DI 10.1007/s00125-015-3498-7 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CF6CL UT WOS:000352644200015 PM 25619168 ER PT J AU Harakotr, B Suriharn, B Scott, MP Lertrat, K AF Harakotr, Bhornchai Suriharn, Bhalang Scott, Marvin Paul Lertrat, Kamol TI Genotypic variability in anthocyanins, total phenolics, and antioxidant activity among diverse waxy corn germplasm SO EUPHYTICA LA English DT Article DE Diversity; Crop breeding; Cluster analysis; Indirect selection; Phytochemicals; Zea mays L ID ZEA-MAYS L.; PURPLE; COLOR; MAIZE; CAPACITY; GRAINS; PLANT; INHERITANCE; PRODUCTS; EXTRACTS AB Identification of germplasm sources of waxy corn (Zea mays L. var. ceratina) with high variability for anthocyanins, phenolic compounds, and antioxidant activity is an important phase for waxy corn breeding for improvement of useful phytochemicals. The objectives of this study were to evaluate 49 genotypes of waxy corn for color parameters, monomeric anthocyanin content (MAC) and total phenolic content (TPC), and antioxidant activities. The experiment was conducted under field conditions in a randomized complete block design with three replications for two seasons in the rainy and the dry season 2010. Corn genotypes and seasons were significantly different (P a parts per thousand currency sign 0.01) for most traits under study except for TPC. Variations due to genotype were large for all characters, accounting for 74.43-95.70 % of total variations. The interactions between genotype and season were significant for all characters. Forty-nine corn genotypes were divided into four groups based on antioxidants and their activities. Significant and positive correlations were found among the anthocyanins, phenolics, and antioxidant activities, and correlation coefficients between anthocyanins with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability and Trolox equivalent antioxidant capacity (TEAC) assays were high (r = 0.94 and 0.88, respectively). All of the Hunterlab color parameters, including chroma and hue angle were highly correlated with anthocyanins, phenolics and their activities and therefore could be used as indirect selection criteria for improving levels of antioxidants and antioxidant activity in waxy corn. C1 [Harakotr, Bhornchai; Suriharn, Bhalang; Lertrat, Kamol] Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. [Harakotr, Bhornchai; Suriharn, Bhalang; Lertrat, Kamol] Khon Kaen Univ, Plant Breeding Res Ctr Sustainable Agr, Khon Kaen 40002, Thailand. [Scott, Marvin Paul] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Lertrat, K (reprint author), Khon Kaen Univ, Fac Agr, Dept Plant Sci & Agr Resources, Khon Kaen 40002, Thailand. EM kamol9@gmail.com FU Royal Golden Jubilee Ph.D. Program (RGJ); National Science and Technology Development Agency, Thailand; Plant Breeding Research Center for Sustainable Agriculture, Faculty of Agriculture, Khon Kaen University, Thailand FX The authors would like to thank for financial support from: the Royal Golden Jubilee Ph.D. Program (RGJ); National Science and Technology Development Agency, Thailand; the Plant Breeding Research Center for Sustainable Agriculture, Faculty of Agriculture, Khon Kaen University, Thailand. We also wish to thanks Assist. Prof. Dr. Sakunkan Simla (Faculty of Technology, Mahasarakham University, Thailand) for collecting data regarding kernel color parameters. Mr. Thawan Kesmala is acknowledged for his generosity in providing constructive comments. NR 40 TC 1 Z9 1 U1 4 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2015 VL 203 IS 2 BP 237 EP 248 DI 10.1007/s10681-014-1240-z PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CG4XP UT WOS:000353292900001 ER PT J AU Spanic, V Rouse, MN Kolmer, JA Anderson, JA AF Spanic, Valentina Rouse, Matthew N. Kolmer, James A. Anderson, James A. TI Leaf and stem seedling rust resistance in wheat cultivars grown in Croatia SO EUPHYTICA LA English DT Article DE Wheat; Gene postulation; Leaf rust; Stem rust ID TRITICUM-AESTIVUM L.; F-SP TRITICI; PUCCINIA-TRITICINA; RACE UG99; MICROSATELLITE MARKERS; HEXAPLOID WHEAT; UNITED-KINGDOM; COMMON WHEAT; GENE; IDENTIFICATION AB Diseases caused by Puccinia species can have devastating effects on wheat and other crops, causing severe economic losses by reducing grain yield and quality. Generally, three strategies are used to control leaf and stem rust wheat diseases: (i) incorporating genetic resistance into new cultivars (ii) the use of fungicides (iii) crop management. The first approach is the most environmentally and economically efficient. Combined with traditional selection techniques, marker-assisted selection (MAS) has become a valuable tool in selection of individuals carrying genes that control traits of interest, such as disease resistance. The objective of this study is to postulate leaf and stem rust resistance genes in 50 mostly Croatian wheat cultivars. Most of the cultivars were screened for the first time for rust diseases. The studies detected six leaf rust resistance genes (Lr2a, Lr3, Lr10, Lr14a, Lr17 and Lr26) and four stem leaf resistance genes (Sr8a, Sr31, Sr36 and Sr38). Nine wheat cultivars had one or more unidentified Lr genes that were not included in the set of 24 isolines, and six wheat cultivars had one or more unidentifed Sr genes. Knowledge on the identity of the seedling rust resistance genes in released cultivars and germplasm is essential for the incorporation of effective resistance genes into breeding programs to maintain a diversity of resistance genes in commonly grown cultivars. C1 [Spanic, Valentina] Agr Inst Osijek, Dept Breeding & Genet Small Cereal Crops, Osijek 31103, Croatia. [Rouse, Matthew N.; Kolmer, James A.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rouse, Matthew N.; Kolmer, James A.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Anderson, James A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. RP Spanic, V (reprint author), Agr Inst Osijek, Dept Breeding & Genet Small Cereal Crops, Juzno Predgradje 17, Osijek 31103, Croatia. EM valentina.spanic@poljinos.hr RI Rouse, Matthew/G-5474-2011 FU Fulbright foundation FX We acknowledge the Fulbright foundation for the support. NR 63 TC 0 Z9 0 U1 7 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2015 VL 203 IS 2 BP 437 EP 448 DI 10.1007/s10681-014-1312-0 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CG4XP UT WOS:000353292900018 ER PT J AU Godinho, VM Goncalves, VN Santiago, IF Figueredo, HM Vitoreli, GA Schaefer, CEGR Barbosa, EC Oliveira, JG Alves, TMA Zani, CL Junior, PAS Murta, SMF Romanha, AJ Kroon, EG Cantrell, CL Wedge, DE Duke, SO Ali, A Rosa, CA Rosa, LH AF Godinho, Valeria M. Goncalves, Vivian N. Santiago, Iara F. Figueredo, Hebert M. Vitoreli, Gislaine A. Schaefer, Carlos E. G. R. Barbosa, Emerson C. Oliveira, Jaquelline G. Alves, Tania M. A. Zani, Carlos L. Junior, Policarpo A. S. Murta, Silvane M. F. Romanha, Alvaro J. Kroon, Erna Geessien Cantrell, Charles L. Wedge, David E. Duke, Stephen O. Ali, Abbas Rosa, Carlos A. Rosa, Luiz H. TI Diversity and bioprospection of fungal community present in oligotrophic soil of continental Antarctica SO EXTREMOPHILES LA English DT Article DE Antarctica; Drug discovery; Ecology; Fungi; Taxonomy ID WINDMILL ISLANDS; PENICILLIUM; MACROALGAE; MICROFUNGI; DERIVATIVES; ANTIFUNGAL; PROGRAMS; TAXONOMY; EXTRACTS; PLANTS AB We surveyed the diversity and capability of producing bioactive compounds from a cultivable fungal community isolated from oligotrophic soil of continental Antarctica. A total of 115 fungal isolates were obtained and identified in 11 taxa of Aspergillus, Debaryomyces, Cladosporium, Pseudogymnoascus, Penicillium and Hypocreales. The fungal community showed low diversity and richness, and high dominance indices. The extracts of Aspergillus sydowii, Penicillium allii-sativi, Penicillium brevicompactum, Penicillium chrysogenum and Penicillium rubens possess antiviral, antibacterial, antifungal, antitumoral, herbicidal and antiprotozoal activities. Bioactive extracts were examined using H-1 NMR spectroscopy and detected the presence of secondary metabolites with chemical shifts. Our results show that the fungi present in cold-oligotrophic soil from Antarctica included few dominant species, which may have important implications for understanding eukaryotic survival in cold-arid oligotrophic soils. We hypothesize that detailed further investigations may provide a greater understanding of the evolution of Antarctic fungi and their relationships with other organisms described in that region. Additionally, different wild pristine bioactive fungal isolates found in continental Antarctic soil may represent a unique source to discover prototype molecules for use in drug and biopesticide discovery studies. C1 [Godinho, Valeria M.; Goncalves, Vivian N.; Santiago, Iara F.; Figueredo, Hebert M.; Vitoreli, Gislaine A.; Kroon, Erna Geessien; Rosa, Carlos A.; Rosa, Luiz H.] Univ Fed Minas Gerais, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Schaefer, Carlos E. G. R.] Univ Fed Vicosa, Dept Ciencia Solo, Vicosa, MG, Brazil. [Barbosa, Emerson C.; Oliveira, Jaquelline G.; Alves, Tania M. A.; Zani, Carlos L.; Junior, Policarpo A. S.; Murta, Silvane M. F.; Romanha, Alvaro J.] Fiocruz MS, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil. [Romanha, Alvaro J.] Univ Fed Santa Catarina, Dept Microbiol Imunol & Parasitol, Florianopolis, SC, Brazil. [Cantrell, Charles L.; Wedge, David E.; Duke, Stephen O.] USDA ARS, Nat Prod Utilizat Res Unit, University, AL USA. [Ali, Abbas] Univ Mississippi, NCNPR, University, MS USA. RP Rosa, LH (reprint author), Univ Fed Minas Gerais, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. EM lhrosa@icb.ufmg.br RI Zani, Carlos/A-9658-2008 OI Zani, Carlos/0000-0003-1859-177X FU CNPq [PROANTAR 407230/2013-0]; CNPq (INCT Criosfera); FAPEMIG [0050-13]; CAPES [23038.003478/2013-92]; FINEP [2084/07]; PDTIS Fiocruz FX We acknowledge the financial support from CNPq (Processes PROANTAR 407230/2013-0 and INCT Criosfera), FAPEMIG (0050-13), CAPES (23038.003478/2013-92), FINEP (2084/07), PDTIS Fiocruz. The authors thank Solomon Green III and Robert D. Johnson for technical assistance. NR 65 TC 6 Z9 6 U1 10 U2 28 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 1431-0651 EI 1433-4909 J9 EXTREMOPHILES JI Extremophiles PD MAY PY 2015 VL 19 IS 3 BP 585 EP 596 DI 10.1007/s00792-015-0741-6 PG 12 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA CG4XY UT WOS:000353294100004 PM 25809294 ER PT J AU Ramsay, JD Evanoff, R Wilkinson, TE Divers, TJ Knowles, DP Mealey, RH AF Ramsay, Joshua D. Evanoff, Ryan Wilkinson, Tom E., Jr. Divers, Thomas J. Knowles, Donald P. Mealey, Robert H. TI Experimental Transmission of Equine Hepacivirus in Horses as a Model for Hepatitis C Virus SO HEPATOLOGY LA English DT Article ID COMBINED IMMUNODEFICIENCY; LIVER-TRANSPLANTATION; INFECTION; DISEASE; SERUM; CHIMPANZEES; CLEARANCE; IDENTIFICATION; DETERMINANTS; PERSISTENCE AB Equine hepacivirus (EHCV; nonprimate hepacivirus) is a hepatotropic member of the Flaviviridae family that infects horses. Although EHCV is the closest known relative to hepatitis C virus (HCV), its complete replication kinetics in vivo have not been described, and direct evidence that it causes hepatitis has been lacking. In this study, we detected EHCV in 2 horses that developed post-transfusion hepatitis. Plasma and serum from these horses were used to experimentally transmit EHCV to 4 young adult Arabian horses, two 1-month-old foals (1 Arabian and 1 Arabian-pony cross), and 2 foals (1 Arabian and 1 Arabian-pony cross) with severe combined immunodeficiency (SCID). Our results demonstrated that EHCV had infection kinetics similar to HCV and that infection was associated with acute and chronic liver disease as measured by elevations of liver-specific enzymes and/or by histopathology. Although most of these animals were coinfected with equine pegivirus (EPgV), also a flavivirus, EPgV viral loads were much lower and often undetectable in both liver and blood. Three additional young adult Arabian-pony crosses and 1 SCID foal were then inoculated with plasma containing only EHCV, and evidence of mild hepatocellular damage was observed. The different levels of liver-specific enzyme elevation, hepatic inflammation, and duration of viremia observed during EHCV infection suggested that the magnitude and course of liver disease was mediated by the virus inoculum and/or by host factors, including breed, age, and adaptive immune status. Conclusion: This work documents the complete infection kinetics and liver pathology associated with acute and chronic EHCV infection in horses and further justifies it as a large animal model for HCV. (Hepatology 2015;61:1533-1546) C1 [Ramsay, Joshua D.; Evanoff, Ryan; Knowles, Donald P.; Mealey, Robert H.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Wilkinson, Tom E., Jr.] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. [Divers, Thomas J.] Cornell Univ, Coll Vet Med, Dept Clin Sci, Ithaca, NY 14853 USA. [Knowles, Donald P.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. RP Ramsay, JD (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, POB 647040, Pullman, WA 99164 USA. EM jdr105@vetmed.wsu.edu; rhm@vetmed.wsu.edu FU Washington State University College of Veterinary Medicine Equine Infectious Diseases Research Program FX This work was supported by the Washington State University College of Veterinary Medicine Equine Infectious Diseases Research Program. NR 30 TC 17 Z9 17 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0270-9139 EI 1527-3350 J9 HEPATOLOGY JI Hepatology PD MAY PY 2015 VL 61 IS 5 BP 1533 EP 1546 DI 10.1002/hep.27689 PG 14 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA CG4DR UT WOS:000353233500014 PM 25580897 ER PT J AU Zelinka, SL Gleber, SC Vogt, S Lopez, GMR Jakes, JE AF Zelinka, Samuel L. Gleber, Sophie-Charlotte Vogt, Stefan Lopez, Gabriela M. Rodriguez Jakes, Joseph E. TI Threshold for ion movements in wood cell walls below fiber saturation observed by X-ray fluorescence microscopy (XFM) SO HOLZFORSCHUNG LA English DT Article DE ionic conduction; micro X-ray fluorescence; percolation; wood anatomy; wood-moisture relations ID ALLOY COATED STEEL; WATER RELATIONS; BEHAVIOR; MOISTURE; DECAY; ZINC AB Diffusion of chemicals and ions through the wood cell wall plays an important role in wood damage mechanisms. In the present work, free diffusion of ions through wood secondary walls and middle lamellae has been investigated as a function of moisture content (MC) and anatomical direction. Various ions (K, Cl, Zn, Cu) were injected into selected regions of 2 mu m thick wood sections with a microinjector and then the ion distribution was mapped by means of X-ray fluorescence microscopy with submicron spatial resolution. The MC of the wood was controlled in situ by means of climatic chamber with controlled relative humidity (RH). For all ions investigated, there was a threshold RH below which the concentration profiles did not change. The threshold RH depended upon ionic species, cell wall layer, and wood anatomical orientation. Above the threshold RH, differences in mobility among ions were observed and the mobility depended upon anatomical direction and cell wall layer. These observations support a recently proposed percolation model of electrical conduction in wood. The results contribute to understanding the mechanisms of fungal decay and fastener corrosion that occur below the fiber saturation point. C1 [Jakes, Joseph E.] US Forest Serv, Forest Biopolymers Sci & Engn, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Zelinka, Samuel L.; Lopez, Gabriela M. Rodriguez] US Forest Serv, Durabil & Wood Protect Res, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Gleber, Sophie-Charlotte; Vogt, Stefan] Argonne Natl Lab, Xray Sci Div, Argonne, IL 60439 USA. RP Jakes, JE (reprint author), US Forest Serv, Forest Biopolymers Sci & Engn, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM jjakes@fs.fed.us RI Vogt, Stefan/B-9547-2009; Vogt, Stefan/J-7937-2013 OI Vogt, Stefan/0000-0002-8034-5513; Vogt, Stefan/0000-0002-8034-5513 FU USDA PECASE Awards; SURE-REU program at UW-Madison; US Department of Energy, Basic Energy Sciences, Office of Science [W-31-109-Eng-38] FX JEJ and SLZ acknowledge funding from 2011 and 2010 USDA PECASE Awards, respectively. GMR acknowledges the SURE-REU program at UW-Madison for support to conduct research during summer 2013. The use of Advanced Photon Source facilities was supported by the US Department of Energy, Basic Energy Sciences, Office of Science, under contract number W-31-109-Eng-38. The authors acknowledge the machine shop at the Forest Products Laboratory for construction of the in situ relative humidity chamber. NR 24 TC 8 Z9 8 U1 0 U2 10 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD MAY PY 2015 VL 69 IS 4 BP 441 EP 448 DI 10.1515/hf-2014-0138 PG 8 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA CG6NR UT WOS:000353419400008 ER PT J AU Miller, BV Lerch, RN Groves, CG Polk, JS AF Miller, Benjamin V. Lerch, Robert N. Groves, Christopher G. Polk, Jason S. TI Recharge mixing in a complex distributary spring system in the Missouri Ozarks, USA SO HYDROGEOLOGY JOURNAL LA English DT Article DE Distributary springs; Karst; Groundwater/surface-water relations; Hydrochemistry; Conceptual models; USA ID KARST; CATCHMENTS; COMPONENTS; TRACERS; NEVADA; STREAM; CHINA AB Toronto Springs is a complex distributary karst spring system with 11 perennial springs in the Missouri Ozarks, USA. Carroll Cave (CC) and Wet Glaize Creek (WG) were previously identified as principal recharge sources. This study (1) characterized physical and chemical properties of springs and recharge sources; (2) developed end-member mixing models to estimate contributing proportions of CC and WG; and (3) created a conceptual model for the system. Samples analyzed for major ions and specific conductivity, in conjunction with a rotating continuous monitoring program to identify statistically comparable baseflow conditions, were used to assess differences among the sites. Monitoring data showed that the springs differed depending upon recharge proportions. Cluster analysis of average ion concentrations supported the choice of CC and WG as mixing model end members. Results showed a range in the proportions of the recharge sources, from surface-water to groundwater dominated. A conceptual model suggests that a system of distinct conduits beneath the WG flood plain transmits water to the individual springs. These conduits controlled the end-member recharge contributions and water chemistry of the springs. Interpretation of relative proportions of recharge contributions extends existing knowledge of karst hydrologic geometry beyond that of point-to-point connections to revealing complex surface-water/groundwater mixing in heterogeneous distributary spring systems. C1 [Miller, Benjamin V.; Groves, Christopher G.; Polk, Jason S.] Western Kentucky Univ, Hoffman Environm Res Inst, Dept Geog & Geol, Bowling Green, KY 42101 USA. [Lerch, Robert N.] Univ Missouri, USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO 65211 USA. RP Miller, BV (reprint author), US Geol Survey, Tennessee Water Sci Ctr, Nashville, TN 37211 USA. EM caverben@yahoo.com FU Missouri Speleological Survey; Cave Research Foundation; Carroll Cave Conservancy; National Speleological Society; Western Kentucky University FX The authors would like to thank the following organizations for their funding support of the project: Missouri Speleological Survey; Cave Research Foundation; Carroll Cave Conservancy; National Speleological Society; and Western Kentucky University. Special thanks to: Amber Spohn for her field work support in sample collection and datalogger maintenance, Lee Anne Bledsoe and Priscilla Baker from the Crawford Hydrology Laboratory for all of their assistance in the various aspects of the groundwater tracing, and Jeffery Crews with the Missouri Geological Survey for his assistance in obtaining information regarding geological mapping that had occurred in the study area. The authors would also like to thank the various landowners who provided access to many of the recharge features and to the numerous cavers who assisted in charcoal packet retrieval, dye injection assistance, and in the ongoing mapping of Carroll Cave. NR 46 TC 2 Z9 2 U1 1 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1431-2174 EI 1435-0157 J9 HYDROGEOL J JI Hydrogeol. J. PD MAY PY 2015 VL 23 IS 3 BP 451 EP 465 DI 10.1007/s10040-014-1225-y PG 15 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA CG3ZN UT WOS:000353219200004 ER PT J AU Miller, BV Lerch, RN Groves, CG Polk, JS AF Miller, Benjamin V. Lerch, Robert N. Groves, Christopher G. Polk, Jason S. TI Recharge mixing in a complex distributary spring system in the Missouri Ozarks, USA (vol 23, pg 451, 2015) SO HYDROGEOLOGY JOURNAL LA English DT Correction C1 [Miller, Benjamin V.; Groves, Christopher G.; Polk, Jason S.] Western Kentucky Univ, Hoffman Environm Res Inst, Dept Geog & Geol, Bowling Green, KY 42101 USA. [Lerch, Robert N.] Univ Missouri, USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO 65211 USA. RP Miller, BV (reprint author), US Geol Survey, Tennessee Water Sci Ctr, Nashville, TN 37211 USA. EM caverben@yahoo.com NR 1 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1431-2174 EI 1435-0157 J9 HYDROGEOL J JI Hydrogeol. J. PD MAY PY 2015 VL 23 IS 3 BP 611 EP 612 DI 10.1007/s10040-015-1253-2 PG 2 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA CG3ZN UT WOS:000353219200013 ER PT J AU Dias, AS Costa, EA Rajao, DS Guedes, RMC Zanella, JRC Lobato, ZIP AF Dias, Alessandra S. Costa, Erica A. Rajao, Daniela S. Guedes, Roberto M. C. Ciacci Zanella, Janice R. Lobato, Zelia I. P. TI Distribution of antibodies against influenza virus in pigs from farrow-to-finish farms in Minas Gerais state, Brazil SO Influenza and Other Respiratory Viruses LA English DT Article DE Antibodies; Brazil; H3N2; pandemic H1N1; serological profile; swine influenza ID SWINE INFLUENZA; UNITED-STATES; A VIRUSES; HERDS; PREVALENCE; INFECTION; ORIGIN AB BackgroundSwine influenza virus (SIV) is the cause of an acute respiratory disease that affects swine worldwide. In Brazil, SIV has been identified in pigs since 1978. After the emergence of pandemic H1N1 in 2009 (H1N1pdm09), few studies reported the presence of influenza virus in Brazilian herds. ObjectivesThe objective of this study was to evaluate the serological profile for influenza virus in farrow-to-finish pig farms in Minas Gerais state, Brazil. MethodsThirty farms with no SIV vaccination history were selected from the four larger pig production areas in Minas Gerais state (Zona da Mata, Triangulo Mineiro/Alto Paranaiba, South/Southwest and the Belo Horizonte metropolitan area). At each farm, blood samples were randomly collected from 20 animals in each production cycle category: breeding animals (sows and gilts), farrowing crate (2-3weeks), nursery (4-7weeks), grower pigs (8-14weeks), and finishing pigs (15-16weeks), with 100 samples per farm and a total of 3000 animals in this study. The samples were tested for hemagglutination inhibition activity against H1N1 pandemic strain (A/swine/Brazil/11/2009) and H3N2 SIV (A/swine/Iowa/8548-2/98) reference strain. ResultsThe percentages of seropositive animals for H1N1pdm09 and H3N2 were 2623% and 157%, respectively, and the percentages of seropositive herds for both viruses were 966% and 132%, respectively. ConclusionsThe serological profiles differed for both viruses and among the studied areas, suggesting a high variety of virus circulation around the state, as well as the presence of seronegative animals susceptible to influenza infection and, consequently, new respiratory disease outbreaks. C1 [Dias, Alessandra S.; Costa, Erica A.; Lobato, Zelia I. P.] Univ Fed Minas Gerais, Dept Vet Prevent Med, Lab Pesquisa Virol Anim, Belo Horizonte, MG, Brazil. [Rajao, Daniela S.] USDA ARS, Virus & Prion Dis Livestock Res Unit, Ames, IA USA. [Guedes, Roberto M. C.] Univ Fed Minas Gerais, Escola Vet, Dept Clin & Cirurgia Vet, Belo Horizonte, MG, Brazil. [Ciacci Zanella, Janice R.] Embrapa Suinos & Aves, Concordia, SC, Brazil. RP Lobato, ZIP (reprint author), Univ Fed Minas Gerais, Dept Vet Prevent Med, Lab Pesquisa Virol Anim, Ave Presidente Antonio Carlos 6627,Cx Postal 567, Belo Horizonte, MG, Brazil. EM ziplobato@gmail.com RI Zanella, Janice/C-3632-2014; OI de Souza Rajao, Daniela/0000-0002-0772-0065 FU CNPq; FAPEMIG (Brazil) FX This study was funded by CNPq and FAPEMIG (Brazil). We thank the Pro-Reitoria de Pesquisa of UFMG, as well as veterinarians Talita Resende, Paulo Oliveira, Flavia Garrocho, and Michele Gabardo for their assistance during sample collection and Aristoteles Gomes Costa for his important contribution. We also thank Jose Eustaquio Cavalcanti for his assistance in contacting all farms and all herd owners for their consent to sample collection. Daniela S. Rajao was a CNPq scholarship recipient. Zelia I.P. Lobato and Roberto M.C. Guedes received research fellowships from CNPq. NR 27 TC 2 Z9 2 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1750-2640 EI 1750-2659 J9 INFLUENZA OTHER RESP JI Influenza Other Respir. Viruses PD MAY PY 2015 VL 9 IS 3 BP 161 EP 167 DI 10.1111/irv.12304 PG 7 WC Infectious Diseases; Virology SC Infectious Diseases; Virology GA CG6JU UT WOS:000353407400009 PM 25648743 ER PT J AU Olanya, OM Annous, BA Taylor, J AF Olanya, Ocen Modesto Annous, Bassam A. Taylor, Janysha TI Effects of Pseudomonas chlororaphis and gaseous chlorine dioxide on the survival of Salmonella enterica on tomatoes SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Gaseous chlorine dioxide; inoculation; Pseudomonas chlororaphis; Salmonella enterica; tomatoes ID ESCHERICHIA-COLI O157/H7; UNITED-STATES; PATHOGENS; CONTAMINATION; FLUORESCENS; BIOCONTROL; INACTIVATION; RHIZOSPHERE; OUTBREAKS; SURFACES AB Control of Salmonella enterica on tomatoes is important for food safety. The aim of this research was to evaluate the survival of Salmonella enterica serovars Montevideo (SM) and Typhimurium (ST) on tomatoes exposed to gaseous chlorine dioxide and Pseudomonas chlororaphis (Pc). Pc was applied to stem scars of tomatoes prior to inoculations with SM and ST. Tomatoes were treated with gaseous ClO2 at 0.4mgL(-1) for 2 and 4h (90% R.H. 13 degrees C), respectively. At 4h of ClO2 treatment, SM and ST populations were reduced to 0.82 and <0.30 log CFUg(-1), respectively. Tomatoes treated with SM and ST had 5.42 and 5.37 log CFUg(-1) of Salmonella. Tomatoes treated with Pc+Salmonella count was 2.59 (treated) and 5.83 log CFUg(-1) (control). Salmonella survival was similar at 2 and 4h of ClO2 treatment. Application of ClO2 and Pc may reduce contamination of tomatoes by Salmonella serovars. C1 [Olanya, Ocen Modesto; Annous, Bassam A.; Taylor, Janysha] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Olanya, OM (reprint author), ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov NR 28 TC 3 Z9 3 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD MAY PY 2015 VL 50 IS 5 BP 1102 EP 1108 DI 10.1111/ijfs.12748 PG 7 WC Food Science & Technology SC Food Science & Technology GA CG9OT UT WOS:000353646700006 ER PT J AU Krauel, JJ Westbrook, JK McCracken, GF AF Krauel, Jennifer J. Westbrook, John K. McCracken, Gary F. TI Weather-driven dynamics in a dual-migrant system: moths and bats SO JOURNAL OF ANIMAL ECOLOGY LA English DT Article DE Chiroptera: Molossidae; community-level impacts; ecosystem services; generalist predators; Helicoverpa zea; Lepidoptera: Noctuidae; Spodoptera frugiperda; synoptic weather; Trichoplusia ni ID FREE-TAILED BATS; HELIOTHIS-VIRESCENS LEPIDOPTERA; HELICOVERPA-ZEA LEPIDOPTERA; TADARIDA-BRASILIENSIS; FALL ARMYWORM; SEASONAL MIGRATION; ECHOLOCATING BATS; TRICHOPLUSIA-NI; INSECT FLIGHT; BLACK CUTWORM AB Animal migrations generate large spatial and temporal fluctuations in biomass that provide a resource base for many predator-prey interactions. These interactions are often driven by continent-scale weather patterns and are difficult to study. Few studies have included migratory animals on more than a single trophic level or for periods spanning multiple entire seasons. We tracked migrations of three species of agricultural pest noctuid moths over the 2010-2012 autumn seasons as the moths travelled past a large colony of migratory Brazilian free-tailed bats (Tadarida brasiliensis) in Texas. Increases in moth abundance, mass of bats and duration of bat activity outside of the cave were correlated with passage of cold fronts over the study area and related increases in northerly wind. Moth responses to weather patterns varied among species and seasons, but overall moth abundances were low in late summer and spiked after one or more cold front passages in September and October. Changes in bat mass and behaviour appear to be consequences of bat migration, as cave use transitioned from summer maternity roost to autumn migratory stopover sites. Weather-driven migration is at considerable risk from climate change, and bat and moth responses to that change may have marked impacts on agricultural systems and bat ecosystem services. C1 [Krauel, Jennifer J.; McCracken, Gary F.] Univ Tennessee, Knoxville, TN 37996 USA. [Westbrook, John K.] ARS, USDA, College Stn, TX 77845 USA. RP Krauel, JJ (reprint author), Univ Tennessee, Knoxville, TN 37996 USA. EM jkrauel@vols.utk.edu OI Krauel, Jennifer/0000-0003-2207-8952; McCracken, Gary/0000-0002-2493-8103 FU Department of Ecology and Evolutionary Biology; James R. Cox Professor Endowment at the University of Tennessee; Bat Conservation International FX We thank J.N. Welch, D. Jones and R. Miller for primary field assistance, N. Troxclair for technical assistance, J. Fordyce for statistical analysis assistance and S. Pedersen for advice, discussion and feedback. We thank J. Chapman and an anonymous reviewer for constructive and helpful feedback. We thank S. Parker and the W. Cofer, B. Gilleland, W. Gilleland and R. King families for generous access to their property, and B. Walker, R. Zaiglin and the students of Southwest Texas Junior College Wildlife Management Programme for additional field assistance. We thank the Department of Ecology and Evolutionary Biology and the James R. Cox Professor Endowment at the University of Tennessee, and Bat Conservation International for funding. All experiments were performed under UTK-IACUC protocol no. 1947 and Texas Scientific Research Permit No. SPR-0810-119, and complied with the current laws of the country in which they were performed. The authors declare that they have no conflict of interest. NR 92 TC 1 Z9 1 U1 12 U2 81 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8790 EI 1365-2656 J9 J ANIM ECOL JI J. Anim. Ecol. PD MAY PY 2015 VL 84 IS 3 BP 604 EP 614 DI 10.1111/1365-2656.12327 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CG6JD UT WOS:000353405300003 PM 25492132 ER PT J AU Rehmani, SF Wajid, A Bibi, T Nazir, B Mukhtar, N Hussain, A Lone, NA Yaqub, T Afonso, CL AF Rehmani, Shafqat Fatima Wajid, Abdul Bibi, Tasra Nazir, Bushra Mukhtar, Nadia Hussain, Abid Lone, Nazir Ahmad Yaqub, Tahir Afonso, Claudio L. TI Presence of Virulent Newcastle Disease Virus in Vaccinated Chickens in Farms in Pakistan SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID GENETIC DIVERSITY; GENOTYPES; POULTRY AB One year after a virulent Newcastle disease virus (vNDV) outbreak in Pakistan, the causative strain was present in vaccinated chickens of multiple farms despite the existence of high-average NDV-specific antibody titers (>4.75 log(2)). The data suggest a possible role of vaccinated birds as reservoirs of vNDV. C1 [Rehmani, Shafqat Fatima; Wajid, Abdul; Bibi, Tasra; Nazir, Bushra; Mukhtar, Nadia; Yaqub, Tahir] Univ Vet & Anim Sci, QOL, Lahore, Pakistan. [Wajid, Abdul; Yaqub, Tahir] Univ Vet & Anim Sci, Inst Biochem & Biotechnol IBBt, Lahore, Pakistan. [Hussain, Abid] Poultry Dis Diagnost Lab, Gujranwala, Punjab, Pakistan. [Lone, Nazir Ahmad; Afonso, Claudio L.] ARS, Exot & Emerging Avian Dis Res Unit, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Afonso, CL (reprint author), ARS, Exot & Emerging Avian Dis Res Unit, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. EM claudio.afonso@ars.usda.gov FU U.S. Department of State (USDA/ARS/BEP/CRDF) [NDV 31063, USDA CRIS 6612-32000-064]; PARB CGS [572] FX Funding has been provided by U.S. Department of State (USDA/ARS/BEP/CRDF) grants NDV 31063, USDA CRIS 6612-32000-064, and PARB CGS project 572. NR 11 TC 5 Z9 5 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAY PY 2015 VL 53 IS 5 BP 1715 EP 1718 DI 10.1128/JCM.02818-14 PG 4 WC Microbiology SC Microbiology GA CG1MK UT WOS:000353036500036 PM 25694525 ER PT J AU Fraley, SE Hall, MB Nennich, TD AF Fraley, S. E. Hall, M. B. Nennich, T. D. TI Effect of variable water intake as mediated by dietary potassium carbonate supplementation on rumen dynamics in lactating dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy cow; potassium; water intake ID CATION-ANION DIFFERENCE; ACID-BASE STATUS; MINERAL SALTS; NITROGEN; SODIUM; FERMENTATION; BALANCE; CATTLE; BICARBONATE; METABOLISM AB Water is a critical nutrient for dairy cows, with intake varying with environment, production, and diet. However, little work has evaluated the effects of water intake on rumen parameters. Using dietary potassium carbonate (K2CO3) as a K supplement to increase water intake, the objective of this study was to evaluate the effect of K2CO3 supplementation on water intake and on rumen parameters of lactating dairy cows. Nine ruminally cannulated, late-lactation Holstein cows (207 +/- 12 d in milk) were randomly assigned to 1 of 3 treatments in a replicated 3 x 3 Latin square design with 18-d periods. Dietary treatments (on a dry matter basis) were no added K2CO3 (baseline dietary K levels of 1.67% dietary K), 0.75% added dietary K, and 1.5% added dietary K. Cows were offered treatment diets for a 14-d adaption period followed by a 4-d collection period. Ruminal total, liquid, and dry matter digesta weights were determined by total rumen evacuations conducted 2 h after feeding on d 4 of the collection period. Rumen fluid samples were collected to determine pH, volatile fatty acids, and NH3 concentrations, and Co-EDTA was used to determine fractional liquid passage rate. Milk samples were collected twice daily during the collection period. Milk, milk fat, and protein yields showed quadratic responses with greatest yields for the 0.75% added dietary K treatment. Dry matter intake showed a quadratic response with 21.8 kg/d for the 0.75% added dietary K treatment and 20.4 and 20.5 kg/d for control and the 1.5% added dietary K treatment, respectively. Water intake increased linearly with increasing K2CO3 supplementation (102.4, 118.4, and 129.3 L/d) as did ruminal fractional liquid passage rate in the earlier hours after feeding (0.118, 0.135, and 0.141 per hour). Total and wet weights of rumen contents declined linearly and dry weight tended to decline linearly as dietary K2CO3 increased, suggesting that the increasing water intake and fractional liquid passage rate with increasing K2CO3 increased the overall ruminal turnover rate. Ruminal ammonia concentrations declined linearly and pH increased linearly as K supplementation increased. As a molar percentage of total volatile fatty acids, acetate increased linearly as dietary K increased, though propionate declined. Increasing dietary K2CO3 and total K in the diets of lactating dairy cows increased water consumption and modified ruminal measures in ways suggesting that both liquid and total ruminal turnover were increased as both water and K intake increased. C1 [Fraley, S. E.; Nennich, T. D.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47906 USA. [Hall, M. B.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. RP Nennich, TD (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47906 USA. EM tnennich@famofeeds.com NR 33 TC 3 Z9 3 U1 0 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2015 VL 98 IS 5 BP 3247 EP 3256 DI 10.3168/jds.2014-8557 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CG4PG UT WOS:000353267900042 PM 25747833 ER PT J AU Walker, MP Evock-Clover, CM Elsasser, TH Connor, EE AF Walker, M. P. Evock-Clover, C. M. Elsasser, T. H. Connor, E. E. TI Short communication: Glucagon-like peptide-2 and coccidiosis alter tight junction gene expression in the gastrointestinal tract of dairy calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE coccidiosis; dairy calf; gene expression; tight junction protein ID INTESTINAL BARRIER FUNCTION; BOVINE MAMMARY-GLAND; GUT; DIARRHEA; RECEPTOR; PIGLETS; PATHWAY; GROWTH; ALPHA AB Tight junction (TJ) proteins are integral factors involved in gut barrier function, and therapy with glucagon-like peptide-2 (GLP-2) enhances gut integrity. Our aim was to assess effects of GLP-2 treatment on mRNA expression of 8 TJ complex proteins in the intestine of dairy calves not infected or infected with Eimeria bovis at 11 + 3 d of age. Mucosal epithelium from jejunum, ileum, and cecum was collected at slaughter from Holstein bull calves assigned to 4 groups: noninfected, buffer-treated (n = 5); noninfected, GLP-2 treated (n = 4); E. bovis-infected, buffer-treated (n = 5); and E. bovis-infected, GLP-2-treated (n = 4). Infected calves were orally dosed with 100,000 to 200,000 sporulated E. bovis oocysts on d 0; GLP-2-treated calves received 50 mu g of GLP-2/kg of body weight subcutaneously twice daily for 10 d beginning on d 18; and buffer-treated calves received an equal injection volume of 0.01 M Na bicarbonate buffer. All calves were killed on d 28. The mRNA expression of coxsackie and adenovirus receptor (CXADR), claudins 1, 2, and 4 (CLDN1, CLDN2, and CLDN4), F11 receptor (F11R), junction adhesion molecule 2 (JAM2), occludin (OCLN), and tight junction protein ZO-1 (TJP1) was determined by real-time quantitative PCR. In jejunum and ileum, an interaction of E. bovis infection and GLP-2 treatment on gene expression was noted. In jejunum of noninfected calves, GLP-2 increased CXADR, CLDN2, OCLN, and TJP1 mRNA expression but had no effect on mRNA expression in infected calves. Treatment with GLP-2 also increased tight junction protein ZO-1 protein expression in jejunum of noninfected calves as determined by immunohistochemistry. In ileum, E. bovis decreased expression of JAM2, OCLN, and TJP1 in buffer-treated calves, and GLP-2 increased TJP1 expression in infected calves. In cecum, E. bovis infection reduced expression of CXADR, CLDN4, F11R, and OCLN, and GLP-2 therapy increased expression of CLDN4, F11R, OCLN, and TJP1. Results are consistent with studies in nonruminants showing decreased expression of TJ complex proteins in the intestinal tract during pathogen-induced diarrhea and increased TJ protein expression in intestinal tissues in response to GLP-2 treatment. In conclusion, E. bovis reduces gene expression of TJ proteins primarily in cecum of calves 28 d postinfection, and GLP-2 increases expression of selected TJ genes in intestinal tissues. Use of GLP-2 to improve gut barrier function in ruminants during pathogen-induced diarrhea warrants additional study. C1 [Walker, M. P.; Evock-Clover, C. M.; Elsasser, T. H.; Connor, E. E.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Connor, EE (reprint author), ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM erin.connor@ars.usda.gov NR 22 TC 3 Z9 3 U1 2 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2015 VL 98 IS 5 BP 3432 EP 3437 DI 10.3168/jds.2014-8919 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CG4PG UT WOS:000353267900056 PM 25726101 ER PT J AU Garcia-Ruiz, A Ruiz-Lopez, FJ Wiggans, GR Van Tassell, CP Montaldo, HH AF Garcia-Ruiz, A. Ruiz-Lopez, F. J. Wiggans, G. R. Van Tassell, C. P. Montaldo, H. H. TI Effect of reference population size and available ancestor genotypes on imputation of Mexican Holstein genotypes SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE imputation; Mexican Holstein; ancestor genotype; reference population ID DAIRY-CATTLE; GENOMIC EVALUATION; ACCURACY; INDIVIDUALS; SELECTION; PANELS AB The effects of reference population size and the availability of information from genotyped ancestors on the accuracy of imputation of single nucleotide polymorphisms (SNP) were investigated for Mexican Holstein cattle. Three scenarios for reference population size were examined: (1) a local population of 2,011 genotyped Mexican Holsteins, (2) animals in scenario 1 plus 866 Holsteins in the US genotype database (GDB) with genotyped Mexican daughters, and (3) animals in scenario 1 and all US GDB Holsteins (338,073). Genotypes from 4 chip densities (2 low density, 1 mid density, and 1 high density) were imputed using findhap (version 3) to the 45,195 markers on the mid- density chip. Imputation success was determined by comparing the numbers of SNP with 1 or 2 alleles missing and the numbers of differently predicted SNP (conflicts) among the 3 scenarios. Imputation accuracy improved as chip density and numbers of genotyped ancestors increased, and the percentage of SNP with 1 missing allele was greater than that for 2 missing alleles for all scenarios. The largest numbers of conflicts were found between scenarios 1 and 3. The inclusion of information from direct ancestors (dam or sire) with US GDB genotypes in the imputation of Mexican Holstein genotypes increased imputation accuracy by 1 percentage point for low- density genotypes and by 0.5 percentage points for high- density genotypes, which was about half the gain found with information from all US GDB Holsteins. A larger reference population and the availability of genotyped ancestors improved imputation; animals with genotyped parents in a large reference population had higher imputation accuracy than those with no or few genotyped relatives in a small reference population. For small local populations, including genotypes from other related populations can aid in improving imputation accuracy. C1 [Garcia-Ruiz, A.; Ruiz-Lopez, F. J.] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Fac Estudios Super Cuautitlan, Mexico City 04510, DF, Mexico. [Garcia-Ruiz, A.; Ruiz-Lopez, F. J.] Ctr Nacl Invest Fisiol & Mejoramiento Anim, Inst Nacl Invest Forestales Agr & Pecuarias, Ajuchitlan 76280, Queretaro, Mexico. [Wiggans, G. R.; Van Tassell, C. P.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Montaldo, H. H.] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico. RP Ruiz-Lopez, FJ (reprint author), Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Fac Estudios Super Cuautitlan, Mexico City 04510, DF, Mexico. EM ruiz.felipe@inifap.gob.mx FU CONACYT; CONARGEN; Incorporation of Genomic Information in the Genetic Evaluation Process of Mexican Dairy Cattle [1056821832] FX This study was supported by CONACYT, CONARGEN, and research project 1056821832 (Incorporation of Genomic Information in the Genetic Evaluation Process of Mexican Dairy Cattle). We thank Asociacion Holstein de Mexico (Queretaro, Mexico) and the Council on Dairy Cattle Breeding (Reynoldsburg, OH) for providing data. The authors thank S. M. Hubbard, (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for technical manuscript review. NR 25 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2015 VL 98 IS 5 BP 3478 EP 3484 DI 10.3168/jds.2014-9132 PG 7 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CG4PG UT WOS:000353267900061 PM 25771055 ER PT J AU Wiggans, GR Su, G Cooper, TA Nielsen, US Aamand, GP Guldbrandtsen, B Lund, MS VanRaden, PM AF Wiggans, G. R. Su, G. Cooper, T. A. Nielsen, U. S. Aamand, G. P. Guldbrandtsen, B. Lund, M. S. VanRaden, P. M. TI Short communication: Improving accuracy of Jersey genomic evaluations in the United States and Denmark by sharing reference population bulls SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE Jersey; genomic evaluation; Denmark; reference population; reliability ID ESTIMATED BREEDING VALUES; PREDICTIONS; RELIABILITY; SELECTION; CATTLE AB The effect on prediction accuracy for Jersey genomic evaluations of Danish and US bulls from using a larger reference population was assessed. Each country contributed genotypes from 1,157 Jersey bulls to the reference population of the other. Data were separated into reference (US only, Danish only, and combined US-Danish) and validation (US only and Danish only) populations. Depending on trait (milk, fat, and protein yields and component percentages; productive life; somatic cell score; daughter pregnancy rate; 14 conformation traits; and net merit), the US reference population included 2,720 to 4,772 bulls and cows with traditional evaluations as of August 2009; the Danish reference population included 635 to 996 bulls. The US validation population included 442 to 712 bulls that gained a traditional evaluation between August 2009 and December 2013; the Danish validation population included 105 to 196 bulls with multitrait across-country evaluations on the US scale by December 2013. Genomic predicted transmitting abilities (GPTA) were calculated on the US scale using a selection index that combined direct genomic predictions with either traditional predicted transmitting ability for the reference population or traditional parent averages (PA) for the validation population and a traditional evaluation based only on genotyped animals. Reliability for GPTA was estimated from the reference population and August 2009 traditional PA and PA reliability. For predic-tion of December 2013 deregressed daughter deviations on the US scale, mean August 2009 GPTA reliability for Danish validation bulls was 0.10 higher when based on the combined US-Danish reference population than when the reference population included only Danish bulls; for US validation bulls, mean reliability increased by 0.02 when Danish bulls were added to the US reference population. Exchanging genotype data to increase the size of the reference population is an efficient approach to increasing the accuracy of genomic prediction when the reference population is small. C1 [Wiggans, G. R.; Cooper, T. A.; VanRaden, P. M.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Su, G.; Guldbrandtsen, B.; Lund, M. S.] Aarhus Univ, Dept Mol Biol & Genet, Ctr Quantitat Genet & Genom, DK-8830 Tjele, Denmark. [Nielsen, U. S.] Danish Agr Advisory Serv, Knowledge Ctr Agr, DK-8200 Aarhus, Denmark. [Aamand, G. P.] Nord Cattle Genet Evaluat, DK-8200 Aarhus, Denmark. RP Wiggans, GR (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM George.Wiggans@ars.usda.gov; Guosheng.Su@agrsci.dk FU Green Development and Demonstration Programme project [3405-10-0137] FX The cooperation of the Council on Dairy Cattle Breeding (Reynoldsburg, OH) in supplying North American pedigree, performance, and genotypic data is acknowledged. The Danish study was a Green Development and Demonstration Programme project (grant 3405-10-0137). Nordic Cattle Genetic Evaluation (Aarhus, Denmark) and VikingGenetics (Randers, Denmark) are acknowledged for providing data. The assistance of S. M. Hubbard (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) in literature review and technical editing is acknowledged. The assistance of one anonymous reviewer in detecting an error in the analysis is gratefully acknowledged. NR 29 TC 2 Z9 2 U1 1 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2015 VL 98 IS 5 BP 3508 EP 3513 DI 10.3168/jds.2014-8874 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA CG4PG UT WOS:000353267900066 PM 25771051 ER PT J AU Schrader, KK Harries, MD Page, PN AF Schrader, Kevin K. Harries, Marcuslene D. Page, Phaedra N. TI Temperature effects on biomass, geosmin, and 2-methylisoborneol production and cellular activity by Nocardia spp. and Streptomyces spp. isolated from rainbow trout recirculating aquaculture systems SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Aquaculture; Geosmin; 2-methylisoborneol; Nocardia; Streptomyces ID HALSTEDII AB Isolates of Nocardia cummidelens, Nocard ia fluminea, Streptomyces albidoflavus, and Streptomyces luridiscabiei attributed as the cause of "earthy-musty" off-flavor in rainbow trout (Oncorhynchus mykiss) raised in recirculating aquaculture systems (RAS) were evaluated for the effect of temperature (10-30 A degrees C) on biomass, geosmin, and 2-methylisoborneol (MIB) production and cellular activity. Cultures of these isolates were monitored over 7 days by measuring culture dry weight, geosmin, and MIB production using solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS), and ATP production via a luminometer. Compared to the other isolates, S. luridiscabiei had significantly (P < 0.05) higher biomass (8.17 +/- A 0.35 mg/mL) at 15 A degrees C (water temperature in the RAS) after 7 days incubation. In addition, S. luridiscabiei produced significantly (P < 0.05) higher geosmin (69,976 +/- A 15,733 ng/L) at 15 A degrees C. At 25 A degrees C and 30 A degrees C, S. albidoflavus produced significantly (P < 0.05) higher geosmin (182,074 +/- A 60,272 ng/L and 399,991 +/- A 102,262 ng/L, respectively). All isolates produced MIB at 15 A degrees C, but S. luridiscabiei produced significantly (P < 0.05) higher MIB (97,143 +/- A 28,972 ng/L) and ATP after 7 days. Therefore, S. luridiscabiei appears to be a likely contributor of geosmin and MIB in the RAS. C1 [Schrader, Kevin K.; Harries, Marcuslene D.; Page, Phaedra N.] ARS, USDA, Nat Prod Utilizat Res Unit, Natl Ctr Nat Prod Res, University, MS 38677 USA. RP Schrader, KK (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, Natl Ctr Nat Prod Res, POB 1848, University, MS 38677 USA. EM kevin.schrader@ars.usda.gov NR 11 TC 0 Z9 0 U1 8 U2 24 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 EI 1476-5535 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD MAY PY 2015 VL 42 IS 5 BP 759 EP 767 DI 10.1007/s10295-015-1600-2 PG 9 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CF9DA UT WOS:000352860600010 PM 25724337 ER PT J AU Altman, J Spahn, J Stoody, EE Rihane, C Casavale, KO Olson, R AF Altman, Jean Spahn, Joanne Stoody, Eve Essery Rihane, Colette Casavale, Kellie O. Olson, Richard TI Laying the Foundation for Expanding the Dietary Guidelines for Americans to Address Children from Birth to 24 Months and Women Who Are Pregnant SO Journal of the Academy of Nutrition and Dietetics LA English DT Editorial Material C1 [Altman, Jean; Spahn, Joanne; Stoody, Eve Essery; Rihane, Colette] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. [Casavale, Kellie O.; Olson, Richard] US Dept HHS, Off Dis Prevent & Hlth Promot, Rockville, MD USA. RP Altman, J (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 2 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAY PY 2015 VL 115 IS 5 BP 693 EP 694 DI 10.1016/j.jand.2015.03.008 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CG5QO UT WOS:000353348600002 PM 25911339 ER PT J AU Cullen, KW Chen, TA Dave, JM Jensen, H AF Cullen, Karen W. Chen, Tzu-An Dave, Jayna M. Jensen, Helen TI Differential Improvements in Student Fruit and Vegetable Selection and Consumption in Response to the New National School Lunch Program Regulations: A Pilot Study SO Journal of the Academy of Nutrition and Dietetics LA English DT Article DE School lunch regulations; Children; Lunch consumption; Fruit; Vegetables ID WASTE; CAFETERIAS; NUTRIENTS; CHILDRENS AB Objective To investigate changes in student food selection and consumption in response to the new National School Lunch Program meal patterns during fall 2011. Design Eight elementary and four intermediate schools in one Houston area school district were matched on free/reduced-price meal eligibility and randomized into control or intervention conditions. Intervention Both intervention and control school cafeterias served the same menu. The intervention school cafeterias posted the new meal pattern daily; students could select one fruit and two vegetable servings per reimbursable meal. Control school students could only select the previous meal pattern: a total of two fruit and vegetable servings per meal. Main outcome measures Students were observed during lunch: student sex and foods selected/consumed were recorded. Diet analysis software was used to calculate energy/food groups selected/consumed. Statistical analyses performed Cochran-Mantel-Haenszel chi(2) tests examined differences in the percent of students selecting each meal component by condition, controlling for sex, grade, and school free/reduced-price meal eligibility. Analysis of covariance assessed differences in amount of energy/food groups selected and consumed, and differences in percent of food groups consumed. Results Observations were conducted for 1,149 elementary and 427 intermediate students. Compared with students in the control schools, significantly more intervention elementary and intermediate school students selected total (P<0.001, P<0.05) and starchy vegetables (P<0.001, P<0.01); more intervention intermediate school students selected fruit (P<0.001), legumes (P<0.05), and protein foods (P<0.01). There were significantly greater amounts of these foods selected and consumed, but no differences in the proportion of the foods consumed by condition. Fewer calories were consumed by elementary and intermediate school intervention students. Conclusions More intervention students selected fruit and vegetables at lunch and consumed them compared with control condition students. Future studies with larger and more diverse student populations are warranted. C1 [Cullen, Karen W.; Chen, Tzu-An; Dave, Jayna M.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Jensen, Helen] Iowa State Univ, Dept Econ, Ames, IA USA. RP Cullen, KW (reprint author), 1100 Bates St, Houston, TX 77030 USA. EM kcullen@bcm.edu OI Jensen, Helen/0000-0001-6878-1729 FU National Institutes of Health [R01HD068349]; USDA/Agricultural Research Service [6250-51000-053] FX This study was supported by funding from the National Institutes of Health to Karen W. Cullen (no. R01HD068349). The project was funded in part by federal funds from the USDA/Agricultural Research Service under cooperative agreement no. 6250-51000-053. NR 26 TC 3 Z9 3 U1 2 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAY PY 2015 VL 115 IS 5 BP 743 EP 750 DI 10.1016/j.jand.2014.10.021 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CG5QO UT WOS:000353348600009 PM 25556770 ER PT J AU Fagerquist, CK Zaragoza, WJ AF Fagerquist, Clifton K. Zaragoza, William J. TI Shiga Toxin 2 Subtypes of Enterohemorrhagic E. coli O157:H-E32511 Analyzed by RT-qPCR and Top-Down Proteomics Using MALDI-TOF-TOF-MS SO JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY LA English DT Article DE Shiga toxin; Escherichia coli; MALDI-TOF-TOF; Top-down proteomics; RT-qPCR; mRNA; Bacteriophage; Antibiotic induction; Food safety ID FLIGHT MASS-SPECTROMETRY; HEMOLYTIC-UREMIC SYNDROME; RIBOSOMAL-PROTEIN S1; ESCHERICHIA-COLI; RAPID IDENTIFICATION; FLUORESCENT ASSAY; GENES; SEQUENCE; VARIANTS; DECAY AB We have measured the relative abundance of the B-subunits and mRNA transcripts of two Stx2 subtypes present in Shiga toxin-producing Escherichia coli (STEC) O157:H- strain E32511 using matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-MS/MS) with post source decay (PSD) and real time-quantitative polymerase chain reaction (RT-qPCR). Stx2a and Stx2c in STEC strain E32511 were quantified from the integrated peak area of their singly charged disulfide-intact B-subunit ions at m/z similar to 7819 and m/z similar to 7774, respectively. We found that the Stx2a subtype was 21-fold more abundant than the Stx2c subtype. The two amino acid substitutions (16D a dagger" 16 N and 24D a dagger" 24A) that distinguish Stx2a from Stx2c not only result in a mass difference of 45 Da between their respective B-subunits but also result in distinctly different fragmentation channels by MS/MS-PSD because both substitutions involve an aspartic acid (D) residue. Importantly, these two substitutions have also been linked to differences in subtype toxicity. We measured the relative abundances of mRNA transcripts using RT-qPCR and determined that the stx2a transcript is 13-fold more abundant than stx2c transcript. In silico secondary structure analysis of the full mRNA operons of stx2a and stx2c suggest that transcript structural differences may also contribute to a relative increase of Stx2a over Stx2c. In consequence, toxin expression may be under both transcriptional and post-transcriptional control. C1 [Fagerquist, Clifton K.; Zaragoza, William J.] ARS, Produce Safety & Microbiol Res Unit, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Fagerquist, CK (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM clifton.fagerquist@ars.usda.gov FU USDA-ARS CRIS [5325-42000-047-00D] FX This research was supported by USDA-ARS CRIS project 5325-42000-047-00D. NR 46 TC 3 Z9 3 U1 2 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1044-0305 EI 1879-1123 J9 J AM SOC MASS SPECTR JI J. Am. Soc. Mass Spectrom. PD MAY PY 2015 VL 26 IS 5 BP 788 EP 799 DI 10.1007/s13361-015-1076-3 PG 12 WC Biochemical Research Methods; Chemistry, Analytical; Chemistry, Physical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA CF9HU UT WOS:000352876100012 PM 25667061 ER PT J AU Hamel, SE Hermanson, JC Cramer, SM AF Hamel, Scott E. Hermanson, John C. Cramer, Steven M. TI Mechanical and time-dependent behavior of wood-plastic composites subjected to bending SO JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS LA English DT Article DE Creep; wood-polymer composite; power law; shear; anisotropic; bimodal ID LINEAR VISCOELASTICITY; SHEAR AB The most popular use of wood-plastic composite (WPC) members in the United States has been as outdoor decking material in residential construction. If the use of these products expands into more structural applications, such as beams and joists, it is imperative that the material's mechanical behavior be understood. Since most of the potential structural uses of this material are as flexural members, it is particularly important that the response to this mode of loading is well characterized. Like many filled polymers, WPCs are anisotropic and bimodal, and thus their shear and two axial moduli (tension and compression) must be determined separately. This study determined the shear and axial moduli of six WPC formulations (mainly polypropylene, high-density polyethylene, and low-density polyethylene) by testing prismatic members in bending at multiple span-to-depth ratios. The initial moduli were determined from constant strain rate tests, and their time dependencies were found using creep tests. The resulting axial-to-shear moduli ratios were shown to be greater than 25 for all formulations. The ratios were relatively constant over time at low stress levels, while decreasing over time at high stress levels. C1 [Hamel, Scott E.] Univ Alaska Anchorage, Anchorage, AK 99508 USA. [Hermanson, John C.] Forest Prod Lab, Madison, WI USA. [Cramer, Steven M.] Univ Wisconsin, Madison, WI USA. RP Hamel, SE (reprint author), Univ Alaska Anchorage, 3211 Providence Dr, Anchorage, AK 99508 USA. EM sehamel@uaa.alaska.edu RI Hermanson, John/K-8106-2015 OI Hermanson, John/0000-0002-3325-6665 FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2005-35103-15230] FX This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (grant number 2005-35103-15230). NR 25 TC 0 Z9 0 U1 5 U2 22 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0892-7057 EI 1530-7980 J9 J THERMOPLAST COMPOS JI J. Thermoplast. Compos. Mater. PD MAY PY 2015 VL 28 IS 5 BP 630 EP 642 DI 10.1177/0892705713486140 PG 13 WC Materials Science, Composites SC Materials Science GA CG7ET UT WOS:000353465900003 ER PT J AU Weber, JG Key, N AF Weber, Jeremy G. Key, Nigel TI Leveraging Wealth from Farmland Appreciation: Borrowing, Land Ownership, and Farm Expansion SO LAND ECONOMICS LA English DT Article ID LIQUIDITY CONSTRAINTS; HOUSEHOLD WEALTH; ENTREPRENEURSHIP; PRICES; CHOICE; RIGHTS; IMPACT; MODEL AB We study how increases in wealth from rapid appreciation of farmland influenced farmer decisions to borrow, buy land, and expand: Exploiting periods of high and low appreciation and a panel data model that allows for correlation between prior growth trends and the share of land owned, we find that a dollar increase in paper wealth led younger farmers to increase real-estate-secured borrowing by 48 cents. Land purchases accompanied the increase in borrowing, supporting the view that collateral-based lending may be contributing to the recent run-up in farmland prices. We find no effect of land wealth on production or acres harvested. (JEL Q15, R14) C1 [Weber, Jeremy G.] Univ Pittsburgh, Grad Sch Publ & Int Affairs, Pittsburgh, PA 15260 USA. [Key, Nigel] ERS, USDA, Washington, DC USA. RP Weber, JG (reprint author), Univ Pittsburgh, Grad Sch Publ & Int Affairs, Pittsburgh, PA 15260 USA. NR 47 TC 1 Z9 1 U1 3 U2 4 PU UNIV WISCONSIN PRESS PI MADISON PA JOURNAL DIVISION, 1930 MONROE ST, 3RD FL, MADISON, WI 53711 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD MAY PY 2015 VL 91 IS 2 BP 344 EP 361 PG 18 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA CG0YT UT WOS:000353000900008 ER PT J AU Le, NA Diffenderfer, MR Thongtang, N Ooi, EMM Barrett, PHR Horvath, KV Dolnikowski, GG Asztalos, BF Schaefer, EJ Brown, WV AF Ngoc-Anh Le Diffenderfer, Margaret R. Thongtang, Nuntakorn Ooi, Esther M. M. Barrett, P. Hugh R. Horvath, Katalin V. Dolnikowski, Gregory G. Asztalos, Bela F. Schaefer, Ernst J. Brown, W. Virgil TI Rosuvastatin Enhances the Catabolism of LDL apoB-100 in Subjects with Combined Hyperlipidemia in a Dose Dependent Manner SO LIPIDS LA English DT Article DE Lipoprotein kinetics; Statins; Metabolism; Plasma sterols; HDL subpopulations ID LOW-DENSITY LIPOPROTEINS; MESSENGER-RNA LEVELS; A-I METABOLISM; APOLIPOPROTEIN B-100; CORONARY-DISEASE; CEBUS MONKEYS; APOPROTEIN-B; CHOLESTEROL; ATORVASTATIN; KINETICS AB Dose-associated effects of rosuvastatin on the metabolism of apolipoprotein (apo) B-100 in triacylglycerol rich lipoprotein (TRL, d < 1.019 g/ml) and low density lipoprotein (LDL) and of apoA-I in high density lipoprotein (HDL) were assessed in subjects with combined hyperlipidemia. Our primary hypothesis was that maximal dose rosuvastatin would decrease the apoB-100 production rate (PR), as well as increase apoB-100 fractional catabolic rate (FCR). Eight subjects received placebo, rosuvastatin 5 mg/day, and rosuvastatin 40 mg/day for 8 weeks each in sequential order. The kinetics of apoB-100 in TRL and LDL and apoA-I in HDL were determined at the end of each phase using stable isotope methodology, gas chromatography-mass spectrometry, and multicompartmental modeling. Rosuvastatin at 5 and 40 mg/day decreased LDL cholesterol by 44 and 54 % (both P < 0.0001), triacylglycerol by 14 % (ns) and 35 % (P < 0.01), apoB by 30 and 36 % (both P < 0.0001), respectively, and had no significant effects on HDL cholesterol or apoA-I levels. Significant decreases in plasma markers of cholesterol synthesis and increases in cholesterol absorption markers were observed. Rosuvastatin 5 and 40 mg/day increased TRL apoB-100 FCR by 36 and 46 % (both ns) and LDL apoB-100 by 63 and 102 % (both P < 0.05), respectively. HDL apoA-I PR increased with low dose rosuvastatin (12 %, P < 0.05) but not with maximal dose rosuvastatin. Neither rosuvastatin dose altered apoB-100 PR or HDL apoA-I FCR. Our data indicate that maximal dose rosuvastatin treatment in subjects with combined hyperlipidemia resulted in significant increases in the catabolism of LDL apoB-100, with no significant effects on apoB-100 production or HDL apoA-I kinetics. C1 [Ngoc-Anh Le; Brown, W. Virgil] Atlanta Vet Affairs Med Ctr, Decatur, GA 30033 USA. [Ngoc-Anh Le; Brown, W. Virgil] Emory Univ, Sch Med, Atlanta, GA 30305 USA. [Diffenderfer, Margaret R.; Thongtang, Nuntakorn; Ooi, Esther M. M.; Horvath, Katalin V.; Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Lipid Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Thongtang, Nuntakorn] Mahidol Univ, Fac Med, Siriraj Hosp, Bangkok 10700, Thailand. [Ooi, Esther M. M.; Barrett, P. Hugh R.] Univ Western Australia, Metab Res Ctr, Sch Med & Pharmacol, Royal Perth Hosp, Perth, WA 6009, Australia. [Dolnikowski, Gregory G.] Tufts Univ, Mass Spectrometry Unit, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Brown, WV (reprint author), Atlanta Vet Affairs Med Ctr, Decatur, GA 30033 USA. EM wbrow925@bellsouth.net RI Barrett, Hugh/B-2745-2011; OI Barrett, Peter Hugh/0000-0003-3223-6125 FU AstraZeneca; PHS from the National Institutes of Health [UL1 RR025008]; US Department of Agriculture Research Service [53-3K-06]; National Institutes of Health [P50 HL083813-01] FX The authors thank the staff of the Clinical Interaction Network of the Atlanta CTSI for their support and dedication in the conduct of the study. The authors are grateful to Dr. James Otvos and the technical staff at LipoScience for their assistance with the NMR analysis and to Dr. Eliana Polisecki and her staff at Boston Heart Diagnostics for their assistance with the plasma sterol analysis. The technical assistance of Wing-Yee Wan, Robert Matera, and Dr. Pimjai Anthanont is much appreciated. This research was supported by an investigator-initiated grant from AstraZeneca to Drs. N.-A. Le and W. V. Brown. Additional support was provided by PHS grant UL1 RR025008 from the National Institutes of Health (N.-A.L., W.V.B.), by US Department of Agriculture Research Service Contract 53-3K-06 (E.J.S.), and by Project Grant P50 HL083813-01 from the National Institutes of Health (E.J.S.). The contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health or the US Department of Agriculture. P.H.R.B. is a fellow of the National Health and Medical Research Council of Australia; E.M.M.O., a postdoctoral research fellow of the National Health and Medical Research Council of Australia; and N.T., a postdoctoral research fellow from Siriraj Hospital, Mahidol University, Thailand. NR 29 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD MAY PY 2015 VL 50 IS 5 BP 447 EP 458 DI 10.1007/s11745-015-4005-0 PG 12 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CG8NY UT WOS:000353566200002 PM 25809021 ER PT J AU Wang, XF Mavrodi, DV Ke, LF Mavrodi, OV Yang, MM Thomashow, LS Zheng, N Weller, DM Zhang, JB AF Wang, Xuefei Mavrodi, Dmitri V. Ke, Linfeng Mavrodi, Olga V. Yang, Mingming Thomashow, Linda S. Zheng, Na Weller, David M. Zhang, Jibin TI Biocontrol and plant growth-promoting activity of rhizobacteria from Chinese fields with contaminated soils SO Microbial Biotechnology LA English DT Article ID PSEUDOMONAS-FLUORESCENS PF-5; INDUCED SYSTEMIC RESISTANCE; ROOT-ASSOCIATED BACTERIA; DAMPING-OFF DISEASE; SP STRAIN SB-K88; BIOLOGICAL-CONTROL; 1-AMINOCYCLOPROPANE-1-CARBOXYLATE DEAMINASE; SP NOV.; PHENAZINE-1-CARBOXYLIC ACID; ARABIDOPSIS-THALIANA AB The aim of this study was to inventory the types of plant growth-promoting rhizobacteria (PGPR) present in the rhizosphere of plants grown in soils contaminated with heavy metals, recalcitrant organics, petroleum sewage or salinity in China. We screened 1223 isolates for antifungal activity and about 24% inhibited Rhizoctonia solani or Sclerotinia sclerotiorum. Twenty-four strains inhibitory to R.solani, Gaeumannomyces graminis var. tritici and/or S.sclerotiorum and representing the dominant morphotypes were assayed for PGPR activity. Seven strains contained phlD, prnD, pltC or phzF genes and produced the antibiotics 2,4-diacetylphloroglucinol, pyrrolnitrin, pyoluteorin and phenazines respectively. Six strains contained acdS, which encodes 1-aminocyclopropane-1-carboxylic acid deaminase. Phylogenetic analysis of 16S rDNA and phlD, phzF and acdS genes demonstrated that some strains identified as Pseudomonas were similar to model PGPR strains Pseudomonas protegensPf-5, Pseudomonas chlororaphis subsp. aureofaciens 30-84 and P.brassicacearumQ8r1-96. Pseudomonas protegens- and P.chlororaphis-like strains had the greatest biocontrol activity against Rhizoctonia root rot and take-all of wheat. Pseudomonas protegens and P.brassicacearum-like strains showed the greatest promotion of canola growth. Our results indicate that strains from contaminated soils are similar to well-described PGPR found in agricultural soils worldwide. Growth-promoting rhizobacteria in polluted soils C1 [Wang, Xuefei; Ke, Linfeng; Zheng, Na; Zhang, Jibin] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China. [Wang, Xuefei; Mavrodi, Olga V.; Yang, Mingming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Mavrodi, Dmitri V.] Univ So Mississippi, Dept Biol Sci, Hattiesburg, MS 39406 USA. [Thomashow, Linda S.; Weller, David M.] ARS, Root Dis & Biol Control Res Unit, USDA, Pullman, WA 99164 USA. RP Zhang, JB (reprint author), Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China. EM david.weller@ars.usda.gov; zhangjb05@163.com FU National Basic Research Program of China (973 Program) [2013CB127504]; International Cooperation Project, the Fundamental Research Funds for the Central Universities, China [2011PY122] FX This work was supported by the National Basic Research Program of China (973 Program, 2013CB127504) and an International Cooperation Project, the Fundamental Research Funds for the Central Universities, China (Grant No. 2011PY122) (2011-2012). NR 71 TC 2 Z9 2 U1 4 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1751-7907 EI 1751-7915 J9 MICROB BIOTECHNOL JI Microb. Biotechnol. PD MAY PY 2015 VL 8 IS 3 BP 404 EP 418 DI 10.1111/1751-7915.12158 PG 15 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CG4EP UT WOS:000353236500005 PM 25219642 ER PT J AU Kamoun, S Furzer, O Jones, JDG Judelson, HS Ali, GS Dalio, RJD Roy, SG Schena, L Zambounis, A Panabieres, F Cahill, D Ruocco, M Figueiredo, A Chen, XR Hulvey, J Stam, R Lamour, K Gijzen, M Tyler, BM Grunwald, NJ Mukhtar, MS Tome, DFA Tor, M Van den Ackerveken, G McDowell, J Daayf, F Fry, WE Lindqvist-Kreuze, H Meijer, HJG Petre, B Ristaino, J Yoshida, K Birch, PRJ Govers, F AF Kamoun, Sophien Furzer, Oliver Jones, Jonathan D. G. Judelson, Howard S. Ali, Gul Shad Dalio, Ronaldo J. D. Roy, Sanjoy Guha Schena, Leonardo Zambounis, Antonios Panabieres, Franck Cahill, David Ruocco, Michelina Figueiredo, Andreia Chen, Xiao-Ren Hulvey, Jon Stam, Remco Lamour, Kurt Gijzen, Mark Tyler, Brett M. Gruenwald, Niklaus J. Mukhtar, M. Shahid Tome, Daniel F. A. Toer, Mahmut Van den Ackerveken, Guido McDowell, John Daayf, Fouad Fry, William E. Lindqvist-Kreuze, Hannele Meijer, Harold J. G. Petre, Benjamin Ristaino, Jean Yoshida, Kentaro Birch, Paul R. J. Govers, Francine TI The Top 10 oomycete pathogens in molecular plant pathology SO MOLECULAR PLANT PATHOLOGY LA English DT Review DE oomycetes plant pathology; microbiology; diversity; genomics ID GRAPEVINE DOWNY MILDEW; IRISH POTATO FAMINE; SUDDEN OAK DEATH; BLISTER RUSTS ALBUGINACEAE; EASTERN UNITED-STATES; 3 CLONAL LINEAGES; NB-LRR GENE; PHYTOPHTHORA-INFESTANS; PLASMOPARA-VITICOLA; ARABIDOPSIS-THALIANA AB Oomycetes form a deep lineage of eukaryotic organisms that includes a large number of plant pathogens which threaten natural and managed ecosystems. We undertook a survey to query the community for their ranking of plant-pathogenic oomycete species based on scientific and economic importance. In total, we received 263 votes from 62 scientists in 15 countries for a total of 33 species. The Top 10 species and their ranking are: (1) Phytophthora infestans; (2, tied) Hyaloperonospora arabidopsidis; (2, tied) Phytophthora ramorum; (4) Phytophthora sojae; (5) Phytophthora capsici; (6) Plasmopara viticola; (7) Phytophthora cinnamomi; (8, tied) Phytophthora parasitica; (8, tied) Pythium ultimum; and (10) Albugo candida. This article provides an introduction to these 10 taxa and a snapshot of current research. We hope that the list will serve as a benchmark for future trends in oomycete research. C1 [Kamoun, Sophien; Furzer, Oliver; Jones, Jonathan D. G.; Petre, Benjamin; Yoshida, Kentaro] Sainsbury Lab, Norwich NR4 7UH, Norfolk, England. [Judelson, Howard S.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Ali, Gul Shad] Univ Florida, Dept Plant Pathol, IFAS, Apopka, FL 32703 USA. [Ali, Gul Shad] Univ Florida, MREC, IFAS, Apopka, FL 32703 USA. [Dalio, Ronaldo J. D.] Ctr Citricultura Sylvio Moreira, Biotechnol Lab, Inst Agron, BR-13490970 Cordeiropolis Sao Paulo, Brazil. [Roy, Sanjoy Guha] West Bengal State Univ, Dept Bot, Kolkata 700126, India. [Schena, Leonardo] Univ Mediterranea, Dipartimento Gest Sistemi Agr & Forestali, I-89122 Reggio Di Calabria, Italy. [Zambounis, Antonios] INRA AgroParisTech, BIOGER CPP UMR1290, F-78850 Thiverval Grignon, France. [Panabieres, Franck] Univ Nice Sophia Antipolis, INRA, UMR1355, CNRS,UMR7254,ISA, F-06903 Sophia Antipolis, France. [Cahill, David] Deakin Univ, Geelong, Vic 3217, Australia. [Ruocco, Michelina] Inst Sustainable Plant Protect IPSP, Portici Div, Italian Natl Res Council CNR, I-80055 Portici, NA, Italy. [Figueiredo, Andreia] Univ Lisbon, Fac Sci, Ctr Biodivers Funct & Integrat Genom, P-1749016 Lisbon, Portugal. [Chen, Xiao-Ren] Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China. [Hulvey, Jon] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. [Stam, Remco; Birch, Paul R. J.] Univ Dundee, Div Plant Sci, Coll Life Sci, James Hutton Inst, Invergowrie DD2 5DA, Scotland. [Lamour, Kurt] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Gijzen, Mark] Agr & Agri Food Canada, London, ON N5V 4T3, Canada. [Tyler, Brett M.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Tyler, Brett M.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gruenwald, Niklaus J.] USDA ARS, Plant Pathol Hort Crops Res Lab, Corvallis, OR 97330 USA. [Mukhtar, M. Shahid] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA. [Mukhtar, M. Shahid] Univ Alabama Birmingham, Nutr Obes Res Ctr, Birmingham, AL 35294 USA. [Tome, Daniel F. A.] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England. [Toer, Mahmut] Univ Worcester, Natl Pollen & Aerobiol Res Unit, Henwick Grove WR2 6AJ, Worcester, England. [Van den Ackerveken, Guido] Univ Utrecht, Dept Biol, Plant Microbe Interact, NL-3584 CH Utrecht, Netherlands. [McDowell, John] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Daayf, Fouad] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada. [Fry, William E.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Lindqvist-Kreuze, Hannele] Int Potato Ctr, Lima 12, Peru. [Meijer, Harold J. G.; Govers, Francine] Wageningen Univ, Lab Phytopathol, NL-16708 PB Wageningen, Netherlands. [Petre, Benjamin] Ctr INRA Nancy Lorraine, INRA, Interact Arbres Microorganismes UMR1136, F-54280 Champenoux, France. [Ristaino, Jean] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. RP Kamoun, S (reprint author), Sainsbury Lab, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England. EM sophien.kamoun@tsl.ac.uk RI Grunwald, Niklaus/K-6041-2013; Govers, Francine/A-5616-2009; Ruocco, Michelina/C-5132-2009; Figueiredo, Andreia/G-2887-2014; Kamoun, Sophien/B-3529-2009; Van den Ackerveken, Guido/B-8568-2011; Jones, Jonathan/J-5129-2012; OI Schena, Leonardo/0000-0002-9737-2593; Daayf, Fouad/0000-0001-8912-0460; Grunwald, Niklaus/0000-0003-1656-7602; Govers, Francine/0000-0001-5311-929X; Ruocco, Michelina/0000-0002-8322-3503; Figueiredo, Andreia/0000-0001-8156-7700; Kamoun, Sophien/0000-0002-0290-0315; Van den Ackerveken, Guido/0000-0002-0183-8978; Jones, Jonathan/0000-0002-4953-261X; Guha Roy, Sanjoy/0000-0002-6159-846X; Stam, Remco/0000-0002-3444-6954; Furzer, Oliver/0000-0002-3536-9970; McDowell, John/0000-0002-9070-4874; Tor, Mahmut/0000-0002-4416-5048; Ristaino, Jean/0000-0002-9458-0514; Yoshida, Kentaro/0000-0002-3614-1759 FU European Research Council (ERC); UK Biotechnology and Biological Sciences Research Council (BBSRC); Gatsby Charitable Foundation; National Council for Scientific and Technological Development (CNPq/CsF Brazil) [313139/2013-0]; Conoscenze Integrate per Sostenibilita ed Innovazione del Made in Italy Agroalimentare (CISIA-MIUR); Italian Ministry of Education, University and Research (MIUR) [FIRB 2010-RBFR10PZ4N]; Portuguese Foundation for Science and Technology [SFRH/BPD/63641/2009]; National Natural Science Foundation of China [31101395]; Jiangsu Province Basic Research Program (Natural Science Foundation) of China [BK2011443]; Agriculture and Food Research Institute of the National Institute of Food and Agriculture of the US Department of Agriculture [2011-68004-30154, 2011-68004-30104]; Agriculture and Agri-Food Canada GRDI program; US Department of Agriculture National Institute of Food and Agriculture [2011-68004-30154]; National Research Initiative Competitive Grants Program [2008-35600-18780]; US Department of Agriculture Agricultural Research Service [CRIS 5358-22000-039-00D]; Northwest Center for Nursery Crop Research; US Forest Service; US Department of Agriculture Agricultural Research Service Floriculture Nursery Initiative; Oregon Department of Agriculture/Oregon Association of Nurseries; UK Biotechnology and Biological Sciences Research Council [BB/G015066/1]; US Department of Agriculture-Agriculture and Food Research Initiative [2009-03008, 2011-68004]; National Science Foundation [ABI-1146819]; Virginia Tech Institute for Critical Technology and Applied Sciences; Natural Sciences and Engineering Research Council of Canada (NSERC); Manitoba Agri-Food Research and Development Initiative (MB-ARDI); McCain Foods; Keystone Potato Producers Association (KPPA); Peak of the Market; Dutch Technology Foundation STW-NWO (VIDI) [10281]; INRA Contrat Jeune Scientifique; European Union [267196]; Food for Thought Program, Wageningen University Fund FX Sophien Kamoun, Oliver Furzer and Jonathan D. G. Jones received funding from the European Research Council (ERC), the UK Biotechnology and Biological Sciences Research Council (BBSRC) and the Gatsby Charitable Foundation. Ronaldo Dalio thanks the National Council for Scientific and Technological Development (CNPq/CsF Brazil-313139/2013-0) for financial support. Michelina Ruocco receives support from Conoscenze Integrate per Sostenibilita ed Innovazione del Made in Italy Agroalimentare (CISIA-MIUR). Leonardo Schena was supported by grant FIRB 2010-RBFR10PZ4N from the Italian Ministry of Education, University and Research (MIUR). Andreia Figueiredo receives support from the Portuguese Foundation for Science and Technology (Grant no SFRH/BPD/63641/2009). Xiao-Ren Chen was financed by the National Natural Science Foundation of China (Grant no. 31101395) and Jiangsu Province Basic Research Program (Natural Science Foundation) of China (Grant no. BK2011443). Howard S. Judelson and Brett M. Tyler were supported by grants 2011-68004-30154 and 2011-68004-30104, respectively, from the Agriculture and Food Research Institute of the National Institute of Food and Agriculture of the US Department of Agriculture. Mark Gijzen was supported by the Agriculture and Agri-Food Canada GRDI program. Niklaus J. Gurnwald was supported by grant 2011-68004-30154 from the US Department of Agriculture National Institute of Food and Agriculture, the National Research Initiative Competitive Grants Program grant 2008-35600-18780, the US Department of Agriculture Agricultural Research Service CRIS 5358-22000-039-00D, the Northwest Center for Nursery Crop Research, the US Forest Service, the US Department of Agriculture Agricultural Research Service Floriculture Nursery Initiative and Oregon Department of Agriculture/Oregon Association of Nurseries. Daniel F.A. Tome is funded by the UK Biotechnology and Biological Sciences Research Council grant BB/G015066/1. John McDowell is supported by the US Department of Agriculture-Agriculture and Food Research Initiative (2009-03008 and 2011-68004), the National Science Foundation (ABI-1146819) and the Virginia Tech Institute for Critical Technology and Applied Sciences. Fouad Daayf received funding from acronyms are: The Natural Sciences and Engineering Research Council of Canada (NSERC), Manitoba Agri-Food Research and Development Initiative (MB-ARDI), McCain Foods, Keystone Potato Producers Association (KPPA) and Peak of the Market. Harold J. G. Meijer is funded by The Dutch Technology Foundation STW-NWO (VIDI grant 10281). Benjamin Petre is supported by an INRA Contrat Jeune Scientifique and has received the support of the European Union, in the framework of the Marie-Curie FP7 COFUND People Programme, through the award of an AgreenSkills fellowship (under grant agreement no267196). Francine Govers receives support from the Food for Thought Program, Wageningen University Fund. NR 233 TC 60 Z9 65 U1 32 U2 151 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD MAY PY 2015 VL 16 IS 4 BP 413 EP 434 DI 10.1111/mpp.12190 PG 22 WC Plant Sciences SC Plant Sciences GA CG5PD UT WOS:000353344000008 PM 25178392 ER PT J AU Keyser, TL Loftis, DL AF Keyser, Tara L. Loftis, David L. TI Stump sprouting of 19 upland hardwood species 1 year following initiation of a shelterwood with reserves silvicultural system in the southern Appalachian Mountains, USA SO NEW FORESTS LA English DT Article DE Oak; Non-oak species; Probability of sprouting; Regeneration; Shelterwood with reserves ID OAK REGENERATION; NORTH-AMERICA; TREE DIAMETER; FOREST; GROWTH; MAPLE; DISTURBANCE; MORTALITY; DYNAMICS; STANDS AB Models that quantify the probability of stump sprouting P(s) and sprout characteristics for predominant tree species in the southern Appalachian Mountains, USA are lacking. In this study, 375 plots (0.01 ha) were installed across five stands in eastern Tennessee and western North Carolina. Trees a parts per thousand yen3.8 cm diameter at 1.37 m above groundline (dbh) were tagged, and dbh and species recorded. Stands were regenerated using a two-aged silvicultural system (residual basal area (BA) < 6.3 m(2) ha(-1)). One growing season post-harvest, we recorded (1) sprouting (yes/no), (2) the number of sprouts per stump (sprout density), and (3) dominant (tallest) sprout height. Sprout density, sprout height, and P(s) were modeled as function of dbh, site index (SI), and BA. For white (Quercus alba L.), black (Quercus velutina Lam.), scarlet (Quercus coccinea Muenchh.), and northern red (Quercus rubra L.) oaks, and sweet birch (Betula lenta L.), yellow buckeye (Aesculus flava Aiton), and red maple (Acer rubrum L.), P(s) was inversely related to dbh. Sprout density was positively related to (1) dbh for red maple, yellow buckeye, sourwood (Oxydendrum arboreum L. (DC)), silverbell (Halesia tetraptera Ellis), and white basswood (Tilia heterophylla Vent.), and (2) SI for scarlet oak. For eight species, sprout height was influenced by dbh, SI, dbh and SI, or dbh and BA. Because stump sprouts are a primary source of regeneration, the information on stump sprouting produced for these 19 species can be used to quantify and predict early stand dynamics following regeneration harvests in these diverse southern Appalachian hardwood stands. C1 [Keyser, Tara L.; Loftis, David L.] US Forest Serv, Southern Res Stn, Bent Creek Expt Forest, USDA, Asheville, NC 28806 USA. RP Keyser, TL (reprint author), US Forest Serv, Southern Res Stn, Bent Creek Expt Forest, USDA, 1577 Brevard Rd, Asheville, NC 28806 USA. EM tkeyser@fs.fed.us; david.loftis@bellsouth.net FU Upland Hardwood Ecology and Management Work Unit of the Southern Research Station, USDA, Forest Service [RWU-4157] FX We are grateful to Virginia Gibbs and Tracy Roof, along with other technicians, for study installation and data collection. This research was funded by the Upland Hardwood Ecology and Management Work Unit of the Southern Research Station (RWU-4157), USDA, Forest Service. Comments from two anonymous reviewers greatly improved this manuscript. NR 53 TC 2 Z9 2 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 EI 1573-5095 J9 NEW FOREST JI New For. PD MAY PY 2015 VL 46 IS 3 BP 449 EP 464 DI 10.1007/s11056-015-9470-z PG 16 WC Forestry SC Forestry GA CG1RO UT WOS:000353051500008 ER PT J AU Chandra, A Verma, PK Islam, MN Grisham, MP Jain, R Sharma, A Roopendra, K Singh, K Singh, P Verma, I Solomon, S AF Chandra, A. Verma, P. K. Islam, M. N. Grisham, M. P. Jain, R. Sharma, A. Roopendra, K. Singh, K. Singh, P. Verma, I. Solomon, S. TI Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage SO PLANT BIOLOGY LA English DT Article DE Cell wall invertases; gene expression; heat map; soluble acid; sucrose phosphate synthase; sugarcane; sucrose synthase ID SOLUBLE ACID INVERTASE; MATURING INTERNODAL TISSUE; PHOSPHATE SYNTHASE; NEUTRAL INVERTASE; PLANT DEVELOPMENT; METABOLISM; STEM; PROTEIN; ENZYME; CANE AB Sucrose synthesis/accumulation in sugarcane is a complex process involving many genes and regulatory sequences that control biochemical events in source-sink tissues. Among these, sucrose synthase (SuSy), sucrose phosphate synthase (SPS), soluble acid (SAI) and cell wall (CWI) invertases are important. Expression of these enzymes was compared in an early (CoJ64) and late (BO91) maturing sugarcane variety using end-point and qRT-PCR. Quantitative RT-PCR at four crop stages revealed high CWI expression in upper internodes of CoJ64, which declined significantly in both top and bottom internodes with maturity. In BO91, CWI expression was high in top and bottom internodes and declined significantly only in top internodes as the crop matured. Overall, CWI expression was higher in CoJ64 than in BO91. During crop growth, there was no significant change in SPS expression in bottom internodes in CoJ64, whereas in BO91 it decreased significantly. Apart from a significant decrease in expression of SuSy in mature bottom internodes of BO91, there was no significant change. Similar SAI expression was observed with both end-point and RT-PCR, except for significantly increased expression in top internodes of CoJ64 with maturity. SAI, being a major sucrose hydrolysing enzyme, was also monitored with end-point PCR expression in internode tissues of CoJ64 and BO91, with higher expression of SAI in BO91 at early crop stages. Enzyme inhibitors, e.g. manganese chloride (Mn++), significantly suppressed expression of SAI in both early- and late-maturing varieties. Present findings enhance understanding of critical sucrose metabolic gene expression in sugarcane varieties differing in content and time of peak sucrose storage. Thus, through employing these genes, improvement of sugarcane sucrose content is possible. C1 [Chandra, A.; Jain, R.; Sharma, A.; Roopendra, K.; Singh, P.; Verma, I.; Solomon, S.] Indian Inst Sugarcane Res, Div Plant Physiol & Biochem, Lucknow 226002, Uttar Pradesh, India. [Verma, P. K.; Islam, M. N.; Singh, K.] Natl Inst Plant Genome Res, New Delhi, India. [Grisham, M. P.] USDA ARS, Sugarcane Res Lab, Houma, LA USA. RP Chandra, A (reprint author), Indian Inst Sugarcane Res, Div Plant Physiol & Biochem, Lucknow 226002, Uttar Pradesh, India. EM amaresh_chandra@rediffmail.com FU DST, Government of India FX The authors are grateful to the directors of both institutes (IISR and NIPGR) for providing the necessary facilities and encouragement. We thank the NCBI and TIGR Plant Transcript Assemblies for making their databases available. Funding from DST, Government of India is duly acknowledged. We also thank two anonymous reviewers for critical comments and suggestions that significantly improved the article. NR 68 TC 2 Z9 2 U1 2 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1435-8603 EI 1438-8677 J9 PLANT BIOLOGY JI Plant Biol. PD MAY PY 2015 VL 17 IS 3 BP 608 EP 617 DI 10.1111/plb.12276 PG 10 WC Plant Sciences SC Plant Sciences GA CG1TN UT WOS:000353057300003 PM 25311688 ER PT J AU Payyavula, RS Shakya, R Sengoda, VG Munyaneza, JE Swamy, P Navarre, DA AF Payyavula, Raja S. Shakya, Roshani Sengoda, Venkatesan G. Munyaneza, Joseph E. Swamy, Prashant Navarre, Duroy A. TI Synthesis and regulation of chlorogenic acid in potato: Rerouting phenylpropanoid flux in HQT-silenced lines SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE chlorogenic acid; flavonols; anthocyanins; hydroxycinnamic acid amides; phenylpropanoid metabolism; potatoes; zebra chip; psyllids ID CANDIDATUS LIBERIBACTER SOLANACEARUM; PHENYLALANINE AMMONIA-LYASE; TRANSGENIC TOBACCO PLANTS; ZEBRA CHIP DISEASE; GENE-EXPRESSION; ANTHOCYANIN BIOSYNTHESIS; METABOLISM; FLAVONOL; ACCUMULATION; PATHWAY AB Chlorogenic acid (CGA) is the major phenolic sink in potato tubers and can constitute over 90% of total phenylpropanoids. The regulation of CGA biosynthesis in potato and the role of the CGA biosynthetic gene hydroxycinnamoyl CoA:quinate hydroxycinnamoyl transferase (HQT) was characterized. A sucrose induced accumulation of CGA correlated with the increased expression of phenylalanine ammonia-lyase (PAL) rather than HQT. Transient expression of the potato MYB transcription factor StAN1 (anthocyanin 1) in tobacco increased CGA. RNAi suppression of HQT resulted in over a 90% reduction in CGA and resulted in early flowering. The reduction in total phenolics and antioxidant capacity was less than the reduction in CGA, suggesting flux was rerouted into other phenylpropanoids. Network analysis showed distinct patterns in different organs, with anthocyanins and phenolic acids showing negative correlations in leaves and flowers and positive in tubers. Some flavonols increased in flowers, but not in leaves or tubers. Anthocyanins increased in flowers and showed a trend to increase in leaves, but not tubers. HQT suppression increased biosynthesis of caffeoyl polyamines, some of which are not previously reported in potato. Decreased PAL expression and enzyme activity was observed in HQT suppressed lines, suggesting the existence of a regulatory loop between CGA and PAL. Electrophysiology detected no effect of CGA suppression on potato psyllid feeding. Collectively, this research showed that CGA in potatoes is synthesized through HQT and HQT suppression altered phenotype and redirected phenylpropanoid flux. C1 [Payyavula, Raja S.; Shakya, Roshani; Navarre, Duroy A.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Sengoda, Venkatesan G.; Munyaneza, Joseph E.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA USA. [Swamy, Prashant] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Navarre, Duroy A.] ARS, USDA, Prosser, WA USA. RP Navarre, DA (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. EM navarrer@wsu.edu FU Washington State Potato Commission FX We thank Nnadozie Oraguzie for the use of the LightCycler (R) 480 and Vijaya Pasapula and Bernardo Chaves for statistical analysis. We thank the Washington State Potato Commission for their support. NR 83 TC 3 Z9 3 U1 6 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD MAY PY 2015 VL 13 IS 4 BP 551 EP 564 DI 10.1111/pbi.12280 PG 14 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CG6HQ UT WOS:000353400800010 PM 25421386 ER PT J AU Hester, PY AL-Ramamneh, DS Makagon, MM Cheng, HW AF Hester, P. Y. AL-Ramamneh, D. S. Makagon, M. M. Cheng, H. W. TI Effect of partial comb and wattle trim on pullet behavior and thermoregulation SO POULTRY SCIENCE LA English DT Article DE trimmed wattle; trimmed comb; pullet; behavior; thermography ID WHITE LEGHORN PULLETS; CHICKEN FOOT; PAIN AB The wattles and comb of chickens are important for thermoregulation allowing for heat exchange during high temperatures. These integumentary tissues are sometimes trimmed to prevent tears if caught on cage equipment and to also improve feed efficiency; however, the procedure itself could be painful to chicks. Our objective was to determine the effect of trimming the comb and wattles on behavior, BW, feed usage, and the surface temperature of pullets. The wattles and comb of Leghorns were partially trimmed at 21 d age (n = 6 cages, 13 chicks each) with another 6 cages serving as controls. Behaviors were recorded 3 times daily for 1 h starting at 0800, 1200, and 1500 using instantaneous scan sampling observations conducted every 5 min prior to, on the d of, and after the trim. Group BW at 21, 28, and 36 d age and the amount of feed used for 7 d beginning at 21 and 29 d age were measured per cage. At 1300 h on d before and after the trim, thermal images of the pullet's beak, comb, eye, wattle, and shank were randomly taken on 3 pullets/cage. Smaller proportions of trimmed chicks were eating and greater proportions were sitting on the d of the trim as compared to the controls with the opposite trend occurring on 4 d post-trim (treatment by age interaction, P = 0.03 and 0.0001, respectively). Standing behavior differed only on the d of the trim where smaller proportions of trimmed pullets stood as compared to intact controls (treatment by age interaction, P = 0.0002). Trimming the comb and wattles did not affect preening, running, BW, feed utilization, and the surface temperature of the pullet. The indices of behavior suggest that on the d of the trim, pullets may have experienced temporary distress, but they returned to normal behavior by 5 h post-trim with no long-term effect on BW, feed usage, or surface body temperature. C1 [Hester, P. Y.; Makagon, M. M.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [AL-Ramamneh, D. S.] Jerash Private Univ, Dept Anim Sci & Food Nutr, Jerash 26150, Jordan. [Cheng, H. W.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Hester, PY (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. EM phester@purdue.edu RI Dey, Kamalesh/E-6568-2017 FU Islamic Development Bank FX The Islamic Development Bank provided financial support to D. S. AL-Ramamneh to visit Purdue University and conduct this study. The care and management of the animals were provided by F. A. Haan and B. D. Little, and the statistical advice was given by M. E. Einstein of Purdue University, West Lafayette, IN. Chicks were donated by Hy-Line Hatchery (Warren, IN). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the USDA. The USDA is an equal opportunity provider and employer. NR 18 TC 1 Z9 1 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2015 VL 94 IS 5 BP 860 EP 866 DI 10.3382/ps/pev066 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA CG5QC UT WOS:000353347200005 PM 25796274 ER PT J AU Jacob, R Branton, SL Evans, JD Leigh, SA Peebles, ED AF Jacob, R. Branton, S. L. Evans, J. D. Leigh, S. A. Peebles, E. D. TI Effects of different vaccine combinations against Mycoplasma gallisepticum on the internal egg and eggshell characteristics of commercial layer chickens SO POULTRY SCIENCE LA English DT Article DE bacterin; commercial layer; egg quality; Mycoplasma gallisepticum; vaccine ID DIETARY POULTRY FAT; F-STRAIN; LAYING HENS; PERFORMANCE-CHARACTERISTICS; INOCULATION OVERLAYS; QUALITY PARAMETERS; SIZE DISTRIBUTION; ONSET; LIVE; INFECTION AB Live F-strain Mycoplasma gallisepticum (FMG) vaccines are presently being used to help control field-strain MG outbreaks. However, they may exert some adverse effects on egg production. Live strains of MG of lesser virulence as well as killed vaccines have little or no effect on egg production, but afford lower levels of protection. This has led to research investigating their use in combination with a subsequent overlay vaccination of FMG given later in the production cycle. In the present study, 2 trials were conducted to investigate the effects of prelay vaccinations of live and killed MG vaccines or their combination, in conjunction with an FMG vaccine overlay after peak production, on the egg characteristics of commercial layers. The following vaccination treatments were administered at 10 wk of age (woa): 1) unvaccinated (Control), 2) MG-Bacterin (MGBac) vaccine, 3) ts-11 strain MG (ts11MG) vaccine, and 4) MGBac and ts11MG combination (MGBac + ts11MG). At 45 woa, half of the birds were overlaid with an FMG vaccine. In each trial, internal egg and eggshell parameters including egg weight (EW), Haugh unit score (HU), eggshell breaking strength (EBS), percentage yolk weight (PYW), percentage albumen weight (PAW), percentage eggshell weight (PSW), eggshell weight per unit surface area (SWUSA), percentage yolk moisture (PYM), and percent total lipids (PYL) were determined at various time periods between 21 and 52 woa. At 28 woa, SWUSA was lower in the ts11MG and MGBac + ts11MG groups compared to the Control group. Conversely, at 43 woa, SWUSA was higher in the ts11MG than in the MGBac group. Between 23 and 43 woa, PYL was higher in the MGBac and ts11MG groups in comparison to the Control group. In conclusion, vaccination with MGBac alone or in combination with ts11MG at 10 woa with or without an FMG vaccine overlay at 45 woa does not adversely affect the internal egg or eggshell quality of commercial layers throughout lay. C1 [Jacob, R.; Peebles, E. D.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. [Branton, S. L.; Evans, J. D.; Leigh, S. A.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Peebles, ED (reprint author), Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. EM d.peebles@msstate.edu FU USDA-Agricultural Research Service; [MIS-322270] FX This is journal article number J-12593 from the Mississippi Agricultural and Forestry Experiment Station supported by MIS-322270.; This study was funded by a grant from the USDA-Agricultural Research Service. The authors express appreciation to Sharon Womack of the Mississippi State University Poultry Science Department and Clint Benoit of the USDA Poultry Research Unit, Mississippi State University, for their assistance in data collection. NR 38 TC 3 Z9 3 U1 1 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2015 VL 94 IS 5 BP 912 EP 917 DI 10.3382/ps/pev060 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA CG5QC UT WOS:000353347200012 PM 25701207 ER PT J AU Huff, GR Huff, WE Rath, NC El-Gohary, FA Zhou, ZY Shini, S AF Huff, G. R. Huff, W. E. Rath, N. C. El-Gohary, F. A. Zhou, Z. Y. Shini, S. TI Efficacy of a novel prebiotic and a commercial probiotic in reducing mortality and production losses due to cold stress and Escherichia coli challenge of broiler chicks SO POULTRY SCIENCE LA English DT Article DE prebiotic; probiotic; brooding; cold stress; Escherichia coli ID RESISTANT STARCH; BROODING TEMPERATURE; LARGE-BOWEL; HEN AGE; GROWTH; PERFORMANCE; RESPONSES; FERMENTATION; YOUNG; PIGS AB Prebiotics consisting of resistant starch may alter intestinal ecology, thus modulating inflammation and increasing intestinal health through increased cecal production of short-chain fatty acids (SCFA). Probiotics may directly alter the intestinal microbiome, resulting in the same effects. We hypothesize that adding prebiotics and probiotics to feed may protect the gut of young chicks under stress. Studies 1, 2, and 3 evaluated treatments in a cold stress (CS) and Escherichia coli (EC) oral challenge to 430 day-old broiler chicks for 3 wk. In study 1, prebiotics were administered as 15% of the diet during the first week only and consisted of the following: Hi-Maize resistant starch (HM), potato starch (PS), or raw potato (RP). In studies 2 and 3, the PS treatment was identical to study 1, and an additional probiotic treatment (PRO) was administered in feed and water. In study 1, PS protected BW during the first week and decreased the mortal-ity of CS/EC-challenged birds during the first week and wk 3, while RP decreased the mortality of warmbrooded birds challenged with EC during the first week. In study 2, PS decreased and PRO increased the main effect mean (MEM) of the first week BW. PS and PRO numerically decreased the feed conversion ratio (FCR) by 23 and 29 points, respectively, in CS/EC-challenged birds with no effects on mortality. In study 3, PS decreased and PRO increased the first week and wk 3 MEM BW. PS numerically increased FCR by 16 points, while PRO decreased FCR by 2 points. Both PS and PRO tended to increase overall mortality, and PRO significantly increased mortality in the CS/EC challenge. These results suggest that the effects of PS may be too variable in this challenge model for further study; however, the PRO treatment improved production values and may have potential as an alternative to antibiotics during the first weeks after hatch. C1 [Huff, G. R.; Huff, W. E.; Rath, N. C.] ARS, USDA, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. [El-Gohary, F. A.] Mansoura Univ, Dept Anim Hyg, Mansoura 35516, Egypt. [Zhou, Z. Y.] Southwest Univ, Dept Vet Med, Chongqing 402460, Rongchang Count, Peoples R China. [Shini, S.] Univ Queensland, Sch Biomed Sci, St Lucia, Qld 4072, Australia. RP Huff, GR (reprint author), ARS, USDA, Poultry Prod & Prod Safety Res Unit, Fayetteville, AR 72701 USA. EM grhuff@uark.edu RI Shini, Shaniko/D-7312-2013 FU USDA Agricultural Research Service FX This study was funded by the USDA Agricultural Research Service. We are grateful to Michaela Mohnl and Biomin for providing the probiotic for this study. We also acknowledge the excellent technical assistance of Sonia Tsai, Scott Zornes, and Wally McDonner (USDA, Agricultural Research Service, Poultry Production and Product Safety Research Unit). NR 43 TC 1 Z9 1 U1 1 U2 24 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2015 VL 94 IS 5 BP 918 EP 926 DI 10.3382/ps/pev068 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CG5QC UT WOS:000353347200013 PM 25743418 ER PT J AU Guthrie, J Mancino, L Lin, CTJ AF Guthrie, Joanne Mancino, Lisa Lin, Chung-Tung Jordan TI Nudging Consumers toward Better Food Choices: Policy Approaches to Changing Food Consumption Behaviors SO PSYCHOLOGY & MARKETING LA English DT Review ID HEALTH CLAIMS; LABEL USE; GUIDANCE-SYSTEM; PUBLIC-HEALTH; NUTRITION EDUCATION; OBESITY; INFORMATION; FRAMEWORK; FAT; COMMUNICATION AB The high prevalence of obesity and its associated illnesses, such as cancer, heart disease and diabetes, has sparked interest in finding ways to encourage consumers to make healthier food choices. At the public level, providing information about why and how to choose a healthy diet has been the typical approach to encouraging behavioral change. Information can also influence food producers and marketers to develop and promote more healthful products, creating an environment in which it is easier for consumers to make healthier dietary choices. Nevertheless, information may not always be effective in improving food choices. One explanation is that nutrition information is complex and difficult to convey in a clear, actionable manner. Also, knowledge, while necessary, may not be sufficient to motivate behavior change. Even when consumers understand nutrition information, competing preferences for tastier, less expensive, or more convenient foods may lead them to make other choices. Findings from psychology, marketing, and behavioral economics research suggest approaches that may enhance the effectiveness of informational approaches and complement informational efforts. More research is needed to further develop these strategies and evaluate their effectiveness. C1 [Guthrie, Joanne; Mancino, Lisa] USDA, Washington, DC USA. [Lin, Chung-Tung Jordan] US FDA, Rockville, MD 20857 USA. RP Guthrie, J (reprint author), ERS, Food Econ Div, USDA, 1400 Independence Ave SW,Mailstop 1800, Washington, DC 20250 USA. EM jguthrie@ers.usda.gov NR 105 TC 7 Z9 7 U1 36 U2 106 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0742-6046 EI 1520-6793 J9 PSYCHOL MARKET JI Psychol. Mark. PD MAY PY 2015 VL 32 IS 5 SI SI BP 501 EP 511 DI 10.1002/mar.20795 PG 11 WC Business; Psychology, Applied SC Business & Economics; Psychology GA CG4EH UT WOS:000353235600003 ER PT J AU Acharya, B Lee, S Mian, MAR Jun, TH McHale, LK Michel, AP Dorrance, AE AF Acharya, Bhupendra Lee, Sungwoo Mian, M. A. Rouf Jun, Tae-Hwan McHale, Leah K. Michel, Andrew P. Dorrance, Anne E. TI Identification and mapping of quantitative trait loci (QTL) conferring resistance to Fusarium graminearum from soybean PI 567301B SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID HEAD BLIGHT RESISTANCE; SUDDEN-DEATH SYNDROME; GIBBERELLA EAR ROT; CYST-NEMATODE; GLYCINE-MAX; SPECIES COMPLEX; HETERODERA-GLYCINES; APHID RESISTANCE; ROOT INFECTION; UNITED-STATES AB A major novel QTL was identified in a recombinant inbred line population derived from a cross of 'Wyandot' x PI 567301B for Fusarium graminearum, a seed and seedling pathogen of soybean. Fusarium graminearum is now recognized as a primary pathogen of soybean, causing root, seed rot and seedling damping-off in North America. In a preliminary screen, 'Wyandot' and PI 567301B were identified with medium and high levels of partial resistance to F. graminearum, respectively. The objective of this study was to characterise resistance towards F. graminearum using 184 recombinant inbred lines (RILs) derived from a cross of 'Wyandot' x PI 567301B. The parents and the RILs of the mapping population were evaluated for resistance towards F. graminearum using the rolled towel assay in a randomized incomplete block design. A genetic map was constructed from 2545 SNP markers and 2 SSR markers by composite interval mapping. One major and one minor QTL were identified on chromosomes 8 and 6, respectively, which explained 38.5 and 8.1 % of the phenotypic variance. The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence. Annotation of this region indicates that there are 39 genes including the Rhg4 locus for soybean cyst nematode (SCN) resistance. Based on previous screens, PI 567301B is susceptible to SCN. Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum. C1 [Acharya, Bhupendra; Dorrance, Anne E.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. [Lee, Sungwoo; Jun, Tae-Hwan; Michel, Andrew P.] Ohio State Univ, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, USDA ARS, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [McHale, Leah K.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. RP Dorrance, AE (reprint author), Ohio State Univ, Dept Plant Pathol, 1680 Madison Ave, Wooster, OH 44691 USA. EM dorrance.1@osu.edu RI McHale, Leah/D-9612-2012 FU United Soybean Board; Ohio Soybean Council through soybean producer's check-off dollars; Molecular Cellular Imaging Center at Ohio Agricultural Research and Development Center; Ohio Bioproducts Innovation Center (OBIC); State and Federal Funds FX This study was funded in part by United Soybean Board and Ohio Soybean Council through soybean producer's check-off dollars. We would like to thank Tim Mendiola for his efforts in developing this population; Andreas Cruz, Anna Dixon, Jaqueline Huzar Novakowiski, Austin Pelyak and Thomas Fitz Gibbon for technical assistance. For SNP genotyping, we acknowledge Vanessa Rashbrook at DNA Technologies, University of California, Davis. We also thank Drs. Perry Cregan and Qijian Song at the Soybean Genomics and Improvement Laboratory, USDA-ARS, Beltsville, MD, for developing the BARCSoySNP6k BeadChip array and for the updated physical positions of the SNPs. In addition, we wish to acknowledge support from the Molecular Cellular Imaging Center at Ohio Agricultural Research and Development Center and the Ohio Bioproducts Innovation Center (OBIC). Salaries and research support for this project was provided by State and Federal Funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University, and USDA-ARS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by The Ohio State University, or the United States Department of Agriculture. NR 83 TC 1 Z9 1 U1 2 U2 30 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2015 VL 128 IS 5 BP 827 EP 838 DI 10.1007/s00122-015-2473-5 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CG5TF UT WOS:000353357200004 PM 25690715 ER PT J AU Warrington, CV Abdel-Haleem, H Hyten, DL Cregan, PB Orf, JH Killam, AS Bajjalieh, N Li, Z Boerma, HR AF Warrington, C. V. Abdel-Haleem, H. Hyten, D. L. Cregan, P. B. Orf, J. H. Killam, A. S. Bajjalieh, N. Li, Z. Boerma, H. R. TI QTL for seed protein and amino acids in the Benning x Danbaekkong soybean population SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; RICE ORYZA-SATIVA; LINKAGE GROUP-I; STORAGE PROTEINS; BETA-CONGLYCININ; OIL CONTENT; YIELD QTL; METHIONINE; REGISTRATION; SELECTION AB We identified QTL associated with protein and amino acids in a soybean mapping population that was grown in five environments. These QTL could be used in MAS to improve these traits. Soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. As a sole dietary source of protein, soybean is deficient in the amino acids lysine (Lys), threonine (Thr), methionine (Met), and cysteine (Cys). Increasing these amino acids would benefit the feed industry. The objective of the present study was to identify quantitative trait loci (QTL) associated with crude protein (cp) and amino acids in the 'Benning' x 'Danbaekkong' population. The population was grown in five southern USA environments. Amino acid concentrations as a fraction of cp (Lys/cp, Thr/cp, Met/cp, Cys/cp, and Met + Cys/cp) were determined by near-infrared reflectance spectroscopy. Four QTL associated with the variation in crude protein were detected on chromosomes (Chr) 14, 15, 17, and 20, of which, a QTL on Chr 20 explained 55 % of the phenotypic variation. In the same chromosomal region, QTL for Lys/cp, Thr/cp, Met/cp, Cys/cp and Met + Cys/cp were detected. At these QTL, the Danbaekkong allele resulted in reduced levels of these amino acids and increased protein concentration. Two additional QTL for Lys/cp were detected on Chr 08 and 20, and three QTL for Thr/cp on Chr 01, 09, and 17. Three QTL were identified on Chr 06, 09 and 10 for Met/cp, and one QTL was found for Cys/cp on Chr 10. The study provides information concerning the relationship between crude protein and levels of essential amino acids and may allow for the improvement of these traits in soybean using marker-assisted selection. C1 [Warrington, C. V.; Abdel-Haleem, H.; Li, Z.; Boerma, H. R.] Univ Georgia, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. [Hyten, D. L.; Cregan, P. B.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Hyten, D. L.] DuPont Pioneer, Johnston, IA 50131 USA. [Orf, J. H.; Killam, A. S.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Bajjalieh, N.] Integrat Nutr Inc, Decatur, IL USA. RP Abdel-Haleem, H (reprint author), ARS, Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85238 USA. EM hussein@uga.edu FU United Soybean Board FX This research was supported by funds allocated to the Georgia Agricultural Experiment Stations and grants from the United Soybean Board. NR 61 TC 5 Z9 5 U1 3 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2015 VL 128 IS 5 BP 839 EP 850 DI 10.1007/s00122-015-2474-4 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CG5TF UT WOS:000353357200005 PM 25673144 ER PT J AU Echeverry-Solarte, M Kumar, A Kianian, S Simsek, S Alamri, MS Mantovani, EE McClean, PE Deckard, EL Elias, E Schatz, B Xu, SS Mergoum, M AF Echeverry-Solarte, Morgan Kumar, Ajay Kianian, Shahryar Simsek, Senay Alamri, Mohammed S. Mantovani, Eder E. McClean, Phillip E. Deckard, Edward L. Elias, Elias Schatz, Blaine Xu, Steven S. Mergoum, Mohamed TI New QTL alleles for quality-related traits in spring wheat revealed by RIL population derived from supernumerary x non-supernumerary spikelet genotypes SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID TRITICUM-AESTIVUM L.; DOUBLED HAPLOID POPULATION; BREAD-MAKING QUALITY; GRAIN PROTEIN-CONTENT; WINTER-WHEAT; YIELD COMPONENTS; AGRONOMIC TRAITS; BAKING QUALITY; CHROMOSOMAL LOCATION; GENETIC-ANALYSIS AB A population developed from an exotic line with supernumerary spikelets was genetically dissected for eight quality traits, discovering new genes/alleles with potential use in wheat breeding programs. Identifying new QTLs and alleles in exotic germplasm is paramount for further improvement of quality traits in wheat. In the present study, an RIL population developed from a cross of an elite wheat line (WCB414) and an exotic genotype with supernumerary spikelets (SS) was used to identify QTLs and new alleles for eight quality traits. Composite interval mapping for 1,000 kernels weight (TKW), kernel volume weight (KVW), grain protein content (GPC), percent of flour extraction (FE) and four mixograph-related traits identified a total of 69 QTLs including 19 stable QTLs. These QTLs were located on 18 different chromosomes (except 4D, 5D, and 6D). Thirteen of these QTLs explained more than 15 % of phenotypic variation (PV) and were considered as major QTLs. In this study, we identified 11 QTLs for TKW (R (2) = 7.2-17.1 %), 10 for KVW (R (2) = 6.7-22.5 %), 11 for GPC (R (2) = 4.7-16.9 %), 6 for FE (R (2) = 4.8-19 %) and 31 for mixograph-related traits (R (2) = 3.2-41.2 %). In this population, several previously identified QTLs for SS, nine spike-related and ten agronomic traits were co-located with the quality QTLs, suggesting pleiotropic effects or close linkage among loci. The traits GPC and mixogram-related traits were positively correlated with SS. Indeed, several loci for quality traits were co-located with QTL for SS. The exotic parent contributed positive alleles that increased PV of the traits at 56 % of loci demonstrating the suitability of germplasm with SS to improve quality traits in wheat. C1 [Echeverry-Solarte, Morgan; Kumar, Ajay; Simsek, Senay; Mantovani, Eder E.; McClean, Phillip E.; Deckard, Edward L.; Elias, Elias; Schatz, Blaine; Mergoum, Mohamed] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Kianian, Shahryar] ARS, USDA, Cereal Dis Lab, St Paul, MN USA. [Alamri, Mohammed S.] King Saud Univ, Dept Food Sci & Nutr, Riyadh 11451, Saudi Arabia. [Xu, Steven S.] ARS, USDA, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND USA. RP Mergoum, M (reprint author), N Dakota State Univ, Dept Plant Sci, POB 6050, Fargo, ND 58105 USA. EM Mohamed.Mergoum@ndsu.edu NR 82 TC 5 Z9 6 U1 4 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2015 VL 128 IS 5 BP 893 EP 912 DI 10.1007/s00122-015-2478-0 PG 20 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CG5TF UT WOS:000353357200009 PM 25740563 ER PT J AU Coopersmith, EJ Bell, JE Cosh, MH AF Coopersmith, Evan J. Bell, Jesse E. Cosh, Michael H. TI Extending the soil moisture data record of the US Climate Reference Network (USCRN) and Soil Climate Analysis Network (SCAN) SO ADVANCES IN WATER RESOURCES LA English DT Article DE Soil moisture; Climate Reference Network; Hydrologic modeling; Genetic algorithms; Soil Climate Analysis Network ID RAINFALL; STATES AB Soil moisture estimates are valuable for hydrologic modeling, drought prediction and management, climate change analysis, and agricultural decision support. However, in situ measurements of soil moisture have only become available within the past few decades with additional sensors being installed each year. Comparing newer in situ resources with older resources, previously required a period of cross-calibration, often requiring several years of data collection. One new technique to improve this issue is to develop a methodology to extend the in situ record backwards in time using a soil moisture model and ancillary available data sets. This study will extend the soil moisture record of the U.S. Climate Reference Network (USCRN) by calibrating a precipitation-driven model during the most recent few years when soil moisture data are available and applying that model backwards temporally in years where precipitation data are available and soil moisture data are not. This approach is validated by applying the technique to the Soil Climate Analysis Network (SCAN) where the same model is calibrated in recent years and validated during preceding years at locations with a sufficiently long soil moisture record. Results suggest that if two or three years of concurrent precipitation and soil moisture time series data are available, the calibrated model's parameters can be applied historically to produce RMSE values less than 0.033 m(3)/m(3). With this approach, in locations characterized by in situ sensors with short or intermittent data records, a model can now be used to fill the relevant gaps and improve the historical record as well. Published by Elsevier Ltd. C1 [Coopersmith, Evan J.; Cosh, Michael H.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Bell, Jesse E.] N Carolina State Univ, NCICS, Asheville, NC 28801 USA. RP Coopersmith, EJ (reprint author), ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. FU NOAA through the Cooperative Institute for Climate and Satellites - North Carolina under Cooperative [NA14NES432003]; NASA Terrestrial Hydrology Program [NNH10ZDA001N-THP] FX The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. This work was supported by NOAA through the Cooperative Institute for Climate and Satellites - North Carolina under Cooperative Agreement NA14NES432003. This work was also supported by the NASA Terrestrial Hydrology Program (NNH10ZDA001N-THP). NR 28 TC 8 Z9 8 U1 3 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD MAY PY 2015 VL 79 BP 80 EP 90 DI 10.1016/j.advwatres.2015.02.006 PG 11 WC Water Resources SC Water Resources GA CF4DX UT WOS:000352500000006 ER PT J AU Haavik, LJ Flint, ML Coleman, TW Venette, RC Seybold, SJ AF Haavik, Laurel J. Flint, Mary L. Coleman, Tom W. Venette, Robert C. Seybold, Steven J. TI Goldspotted oak borer effects on tree health and colonization patterns at six newly-established sites SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Agrilus auroguttatus; brood trees; insect population dynamics; invasion dynamics; newly-established invasive species; oak pest; per capita rate of increase ID EMERALD ASH BORER; TWOLINED CHESTNUT BORER; SCHAEFFER COLEOPTERA BUPRESTIDAE; AGRILUS-BILINEATUS COLEOPTERA; ASIAN LONGHORNED BEETLE; SOUTHERN CALIFORNIA; UNITED-STATES; COXALIS WATERHOUSE; WEBER COLEOPTERA; SIREX-NOCTILIO AB Newly-established populations of invasive wood-inhabiting insects provide an opportunity for the study of invasion dynamics and for collecting information to improve management options for these cryptic species. From 2011 to 2013, we studied the dynamics of the goldspotted oak borer Agrilus auroguttatusSchaeffer (Coleoptera: Buprestidae), a new pest of oaks in southern California, at six sites that had been colonized recently. At all sites, the percentage of coast live oaks Quercus agrifoliaNee, colonized by A. auroguttatus increased between 2011 (6-33%) and 2013 (23-40%), although beetle densities did not grow rapidly at most sites. From 2011 to 2013, there were minor changes in signs and symptoms of A. auroguttatus infestation (adult emergence holes, bark staining, and evidence of woodpecker foraging), except at one site where an outbreak occurred. At some sites, noticeable negative changes in oak crown health occurred 1year prior to positive A. auroguttatus population growth. Among sites, most of the A. auroguttatus population density (66-93%) was produced by a small number of heavily-infested trees (=brood trees). Early identification and removal of brood trees in newly-invaded areas could slow the growth of A. auroguttatus populations. C1 [Haavik, Laurel J.; Flint, Mary L.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Coleman, Tom W.] US Forest Serv, USDA, Forest Hlth Protect, San Bernardino, CA 92408 USA. [Venette, Robert C.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95616 USA. RP Haavik, LJ (reprint author), Great Lakes Forestry Ctr, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM ljhaavik@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 FU Goldspotted oak borer survey and technology development [8CA11400]; Cal Fire; UC-Davis; Special Technology Development Program from the USDA Forest Service, Forest Health Protection and Research and Development [R5-2010-02]; USDA Forest Service Northern and Pacific Southwest Research Stations; USDA Forest Service, Forest Health Protection-Pacific Southwest Region; University of California-Davis FX We thank Mike Jones (UC-Davis) and Vanessa Lopez (UC-Riverside) for field assistance; the Boy Scouts of America-San Diego-Imperial Council, San Diego County Parks, and the Cleveland National Forest for access to field sites; Zachary Heath (USDA Forest Service) for creation of Fig. 1; and Kevin Dodds and Andrew Liebhold (both USDA Forest Service) and three anonymous reviewers for helpful comments on an earlier version of the manuscript. This work was supported by a the grant 'Goldspotted oak borer survey and technology development', Agreement #8CA11400 between Cal Fire and UC-Davis; by a Special Technology Development Program grant (#R5-2010-02) from the USDA Forest Service, Forest Health Protection and Research and Development; by the USDA Forest Service Northern and Pacific Southwest Research Stations; by the USDA Forest Service, Forest Health Protection-Pacific Southwest Region; and by the University of California-Davis. We appreciate the assistance of Tom Smith (Cal Fire Forest Pest Management staff) for facilitating the funding for the project. NR 53 TC 4 Z9 4 U1 1 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2015 VL 17 IS 2 BP 146 EP 157 DI 10.1111/afe.12090 PG 12 WC Entomology SC Entomology GA CF4SK UT WOS:000352541300005 ER PT J AU Faccoli, M Favaro, R Smith, MT Wu, JQ AF Faccoli, Massimo Favaro, Riccardo Smith, Michael T. Wu, Jinquan TI Life history of the Asian longhorn beetle Anoplophora glabripennis (Coleoptera Cerambycidae) in southern Europe SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Asian longhorn beetle; biology; invasive species; life history; phenology; survival ID GLOBAL LAND AREAS; MOTSCHULSKY COLEOPTERA; TEMPERATURE; DISPERSAL; CLIMATE; CHINA AB The Asian longhorn beetle Anoplophora glabripennis is highly polyphagous and widely spread over regions with different climates. Determining the key life-history traits is important for understanding how local conditions affect its successful establishment and to develop adaptive management strategies. Field and laboratory studies were conducted from 2010 to 2012 on an A. glabripennis infestation in Northern Italy, aiming to determine its seasonal phenology, adult beetle longevity, density of successful emergence, infestation age and overwintering life history. Adult beetle emerged from infested trees from 22 May to 28 June. Ninety percent of emergence was reached around 20 July. The first 1% of emergence was accurately predicted by an accumulated degree-day model. In the laboratory, the mean longevity of males and females developed under natural conditions was 27.8 +/- 1.7 and 24.9 +/- 1.8days, respectively. In northern Italy, A. glabripennis largely overwinter as mature larvae in the xylem. The mean density of exit holes was 24.0 +/- 2.7 holes/m(2) of bark, with successful emergence from branches as small as 3.2cm in diameter. Although the infestation was discovered in June 2009, the oldest exit hole found in infested trees dated from 2005. C1 [Faccoli, Massimo; Favaro, Riccardo] Univ Padua, Dept Agron Food Nat Resources Anim & Environm, I-35020 Legnaro, PD, Italy. [Smith, Michael T.] ARS, Beneficial Insects Intro Res Unit, USDA, Newark, DE 19711 USA. [Wu, Jinquan] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. RP Faccoli, M (reprint author), Univ Padua, Dept Agron Food Nat Resources Anim & Environm, Viale Univ 16, I-35020 Legnaro, PD, Italy. EM massimo.faccoli@unipd.it FU Italian national project 'Insects and globalization: sustainable control of exotic species in agro-forestry ecosystems (GEISCA)' of the Italian Ministry of University and Research FX We thank the Regional Plant Protection Organizations and the Regional Forest Service of the Veneto Region for facilities and logistic support during the field work. This study was partially supported by the Italian national project 'Insects and globalization: sustainable control of exotic species in agro-forestry ecosystems (GEISCA)' of the Italian Ministry of University and Research. We are grateful to the anonymous reviewers for their valuable comments on an earlier version of the manuscript. Special thanks are extended to Alison Garside for checking the English language. We are grateful to Dr Douglas Luster, USDA-ARS, Beneficial Insect Introductory Research Unit, Newark, DE (U.S.A.), for his critical review of the manuscript. NR 50 TC 6 Z9 6 U1 2 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2015 VL 17 IS 2 BP 188 EP 196 DI 10.1111/afe.12096 PG 9 WC Entomology SC Entomology GA CF4SK UT WOS:000352541300010 ER PT J AU Thomson, JL Tussing-Humphreys, LM Goodman, MH Zoellner, JM AF Thomson, Jessica L. Tussing-Humphreys, Lisa M. Goodman, Melissa H. Zoellner, Jamie M. TI Engagement Indicators Predict Health Changes in a Lifestyle Intervention SO AMERICAN JOURNAL OF HEALTH BEHAVIOR LA English DT Article DE diet; nutrition education; physical activity; African American; participant engagement ID DIETARY INTERVENTION; PHYSICAL-ACTIVITY; ADHERENCE; IMPLEMENTATION; OUTCOMES; BODY AB Objective: To evaluate the utility of several participant engagement indicators for predicting health changes in a church-based lifestyle intervention shown effective for improving dietary, physical activity, and clinical outcomes. Methods: Descriptive indicators were constructed using 2 participant engagement measures - education session attendance (EDA) and exercise class attendance (EXA) - separately and combined. Relationships of 6 engagement indicators to health outcomes were tested using generalized linear mixed models. Results: EDA predicted 5 dietary and 1 clinical outcome, whereas EXA predicted one physical activity and one clinical outcome. The combined indicator predicted the same 7 outcomes. Conclusion: Use of single engagement indicators specific to each intervention component is advocated for predicting relevant health outcome. C1 [Thomson, Jessica L.; Goodman, Melissa H.] ARS, USDA, Stoneville, MS USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Dept Med, Chicago, IL USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Ctr Canc, Chicago, IL USA. [Zoellner, Jamie M.] Virginia Tech, Dept Human Nutr Foods & Exercise, Blacksburg, VA USA. RP Thomson, JL (reprint author), ARS, USDA, Stoneville, MS USA. EM jessica.thomson@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service [640151000-001-00D]; US Department of Health and Human Services, Health Resources Services Administration [6 U1FRH07411] FX We thank the original investigators who developed the intervention, Delta Body and Soul III research team, and Delta Health Alliance. We are particularly grateful to the church leaders, committee members, and study participants for their tremendous support. This research was supported by the US Department of Agriculture, Agricultural Research Service Project 640151000-001-00D and US Department of Health and Human Services, Health Resources Services Administration grant no. 6 U1FRH07411. The views expressed are solely those of the authors and do not reflect the official policy or position of the US government. NR 24 TC 2 Z9 2 U1 0 U2 6 PU PNG PUBLICATIONS PI OAK RIDGE PA 2205-K OAK RIDGE RD, #115, OAK RIDGE, NC 27310 USA SN 1945-7359 J9 AM J HEALTH BEHAV JI Am. J. Health Behav. PD MAY PY 2015 VL 39 IS 3 BP 409 EP 420 DI 10.5993/AJHB.39.3.13 PG 12 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CF5UD UT WOS:000352621700013 PM 25741685 ER PT J AU Sui, Y Wisniewski, M Droby, S Liu, J AF Sui, Yuan Wisniewski, Michael Droby, Samir Liu, Jia TI Responses of Yeast Biocontrol Agents to Environmental Stress SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Review ID PICHIA-ANOMALA J121; CANDIDA-SAKE CPA-1; OXIDATIVE STRESS; BIOLOGICAL-CONTROL; POSTHARVEST DISEASES; METSCHNIKOWIA-FRUCTICOLA; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; ANTAGONISTIC YEAST; LIQUID FORMULATION AB Biological control of postharvest diseases, utilizing wild species and strains of antagonistic yeast species, is a research topic that has received considerable attention in the literature over the past 30 years. In principle, it represents a promising alternative to chemical fungicides for the management of postharvest decay of fruits, vegetables, and grains. A yeast-based biocontrol system is composed of a tritrophic interaction between a host (commodity), a pathogen, and a yeast species, all of which are affected by environmental factors such as temperature, pH, and UV light as well as osmotic and oxidative stresses. Additionally, during the production process, biocontrol agents encounter various severe abiotic stresses that also impact their viability. Therefore, understanding the ecological fitness of the potential yeast biocontrol agents and developing strategies to enhance their stress tolerance are essential to their efficacy and commercial application. The current review provides an overview of the responses of antagonistic yeast species to various environmental stresses, the methods that can be used to improve stress tolerance and efficacy, and the related mechanisms associated with improved stress tolerance. C1 [Sui, Yuan; Liu, Jia] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei, Peoples R China. [Wisniewski, Michael] USDA ARS, Kearneysville, WV USA. [Droby, Samir] Agr Res Org, Yolcanl Ctr, IL-50250 Bet Dagan, Israel. RP Liu, J (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei, Peoples R China. EM jialiu1933@163.com FU National Natural Science Foundation of China [31401794]; Department of Education in Anhui Province [KJ2014ZD25]; Hefei University of Technology [58-1931-4-008FN]; USDA [58-1931-4-008FN] FX This work was supported by National Natural Science Foundation of China (no. 31401794), a project from Department of Education in Anhui Province (no. KJ2014ZD25), and a collaborative project between Hefei University of Technology and USDA (no. 58-1931-4-008FN). NR 91 TC 14 Z9 14 U1 9 U2 70 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2015 VL 81 IS 9 BP 2968 EP 2975 DI 10.1128/AEM.04203-14 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CF6FM UT WOS:000352652400001 PM 25710368 ER PT J AU Agindotan, BO Domier, LL Bradley, CA AF Agindotan, Bright O. Domier, Leslie L. Bradley, Carl A. TI Detection and characterization of the first North American mastrevirus in switchgrass SO ARCHIVES OF VIROLOGY LA English DT Article DE Bioenergy crop; Panicum virgatum; Biomass crop; Mastrevirus; DNA virus ID ALIGNMENT; CROPS; RNA AB Virus infections have the potential to reduce biomass yields in energy crops, including Panicum virgatum (switchgrass). As a first step towards managing virus-induced biomass reduction, deep sequencing was used to identify viruses associated with mosaic symptoms in switchgrass. Two sequences with homology to mastreviruses were identified. Total DNA extracted from switchgrass varieties 'Dewey Blue' and 'Cloud Nine' was used as template to amplify mastrevirus DNA by the rolling-circle method. Complete mastrevirus genome sequences were obtained from cloned amplicons. The two nucleotide sequences were 88 % identical to each other but only 56-57 % identical to the closest relatives in the genus Mastrevirus. Predicted amino acid sequences of the coat protein, replication-associated protein A, replication-associated protein, and putative movement protein encoded by the two mastrevirus-like sequences were 95 %, 79 %, 79 %, and 87 % identical to each other, respectively, and 46-48 %, 31 %, 31 %, and 42-48 % identical to those of the closest mastrevirus relatives. Based on a genome-wide identity threshold of 75 % set by the International Committee on Taxonomy of Viruses and phylogenetic analyses, the two virus sequences appear to represent a new mastrevirus species. The mastrevirus is tentatively named switchgrass mosaic-associated virus 1 (SgMaV-1) and is the first mastrevirus reported from North America. C1 [Agindotan, Bright O.; Bradley, Carl A.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [Domier, Leslie L.] Univ Illinois, USDA, ARS, Urbana, IL 61801 USA. [Domier, Leslie L.; Bradley, Carl A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Agindotan, BO (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM bright.agindotan@montana.edu FU Energy Biosciences Institute FX This work was funded by the Energy Biosciences Institute. NR 12 TC 1 Z9 1 U1 2 U2 6 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD MAY PY 2015 VL 160 IS 5 BP 1313 EP 1317 DI 10.1007/s00705-015-2367-5 PG 5 WC Virology SC Virology GA CF8FB UT WOS:000352790400020 PM 25721298 ER PT J AU Beuch, U Berlin, S Akerblom, J Nicolaisen, M Nielsen, SL Crosslin, JM Hamm, PB Santala, J Valkonen, JPT Kvarnheden, A AF Beuch, Ulrike Berlin, Sofia Akerblom, Jonas Nicolaisen, Mogens Nielsen, Steen Lykke Crosslin, James M. Hamm, Philip B. Santala, Johanna Valkonen, Jari P. T. Kvarnheden, Anders TI Diversity and evolution of potato mop-top virus SO ARCHIVES OF VIROLOGY LA English DT Article DE Genetic variability; Phylogeny; Plant disease; Potato mop-top virus; Selection pressure; Solanum tuberosum ID PROTEIN-ENCODING RNA; NUCLEOTIDE-SEQUENCE; MAXIMUM-LIKELIHOOD; DNA POLYMORPHISM; GENE; PMTV; IDENTIFICATION; WASHINGTON; MOVEMENT; REVEALS AB Nearly complete sequences of RNA-CP and 3'-proximal RNA-TGB were determined for 43 samples of potato mop-top virus (PMTV) originating from potato tubers and field soil from Sweden, Denmark and the USA. The results showed limited diversity and no strict geographical grouping, suggesting only a few original introductions of PMTV from the Andes. Two distinguishable types of RNA-CP and RNA-TGB were found in the samples, but no specific combination of them correlated with spraing symptoms in tubers. Lack of positive selection in the coding sequences indicates that there is no specific molecular adaptation of PMTV to new vectors or hosts. C1 [Beuch, Ulrike; Berlin, Sofia; Akerblom, Jonas; Kvarnheden, Anders] Swedish Univ Agr Sci, Linnean Ctr Plant Biol Uppsala, Uppsala BioCtr, Dept Plant Biol, S-75007 Uppsala, Sweden. [Nicolaisen, Mogens; Nielsen, Steen Lykke] Aarhus Univ, Dept Agroecol, AU Flakkebjerg, DK-4200 Slagelse, Denmark. [Crosslin, James M.] USDA ARS, Prosser, WA 99350 USA. [Hamm, Philip B.] Oregon State Univ, Agr Res & Extens Ctr, Hermiston, OR USA. [Santala, Johanna; Valkonen, Jari P. T.] Univ Helsinki, Dept Agr Sci, Helsinki, Finland. RP Kvarnheden, A (reprint author), Swedish Univ Agr Sci, Linnean Ctr Plant Biol Uppsala, Uppsala BioCtr, Dept Plant Biol, Box 7080, S-75007 Uppsala, Sweden. EM anders.kvarnheden@slu.se RI Nicolaisen, Mogens/H-3574-2016; OI Nicolaisen, Mogens/0000-0002-0407-2488; Valkonen, Jari/0000-0002-3704-9911 FU Nordic Joint Committee for Agricultural Research [NKJ-122]; Swedish Farmers' Foundation for Agricultural Research; Kungliga Fysiografiska Sallskapet i Lund; Viikki Doctoral Programme of Molecular Sciences FX The authors thank Dr. Paula Persson, Ms. Rue Snell, Dr. Willie Kirk and Dr. Jonathan Whitworth for providing samples. Financial support from the Nordic Joint Committee for Agricultural Research (grant no. NKJ-122), Swedish Farmers' Foundation for Agricultural Research, Kungliga Fysiografiska Sallskapet i Lund and The Viikki Doctoral Programme of Molecular Sciences is gratefully acknowledged. NR 28 TC 4 Z9 4 U1 4 U2 15 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD MAY PY 2015 VL 160 IS 5 BP 1345 EP 1351 DI 10.1007/s00705-015-2381-7 PG 7 WC Virology SC Virology GA CF8FB UT WOS:000352790400025 PM 25753427 ER PT J AU Reeves, JL Blumenthal, DM Kray, JA Derner, JD AF Reeves, Justin L. Blumenthal, Dana M. Kray, Julie A. Derner, Justin D. TI Increased seed consumption by biological control weevil tempers positive CO2 effect on invasive plant (Centaurea diffusa) fitness SO BIOLOGICAL CONTROL LA English DT Article DE Climate change; Diffuse knapweed; Larinus minutus; Phenology; Warming ID ATMOSPHERIC CARBON-DIOXIDE; ENEMY RELEASE HYPOTHESIS; ELEVATED CO2; CLIMATE-CHANGE; SPOTTED KNAPWEED; HERBIVORE INTERACTIONS; INSECT INTERACTIONS; SEMIARID GRASSLAND; BIOTIC RESISTANCE; LARINUS-MINUTUS AB Predicted increases in atmospheric CO2 and temperature may benefit some invasive plants, increasing the need for effective invasive plant management. Biological control can be an effective means of managing invasive plants, but the anticipated range in responses of plant-insect interactions to climate change make it difficult to predict how effective biological control will be in the future. Field experiments that manipulate climate within biological control systems could improve predictive power, but are challenging to implement and therefore rare to date. Here, we show that free air CO2 enrichment in the field increased the fitness of Centaurea diffusa Lam., a problematic invader in much of the western United States. However, CO2 enrichment also increased the impact of the biological control agent Larinus minutus (Coleoptera: Curculionidae) on C. diffusa fitness. C. diffusa plants flowered earlier and seed heads developed faster with both elevated CO2 and increased temperature. Natural dispersal of L. minutus into the experimental plots provided a unique opportunity to examine weevil preference for and effects on C. diffusa grown under the different climate change treatments. Elevated CO2 increased both the proportion of seed heads infested by L. minutus and, correspondingly, the amount of seed removed by weevils. Warming had no detectable effect on weevil utilization of plants. Higher weevil densities on elevated CO2 plants reduced, but did not eliminate, the positive effects of CO2 on C. diffusa fitness. Correlations between plant development time and weevil infestation suggest that climate change increased weevil infestation by hastening plant phenology. Phenological mismatches are anticipated with climate change in many plant-insect systems, but in the case of L. minutus and C. diffusa in mixed-grass prairie, a better phenological match may make the biological control agent more effective as CO2 levels rise. Published by Elsevier Inc. C1 [Reeves, Justin L.; Blumenthal, Dana M.; Kray, Julie A.; Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. RP Reeves, JL (reprint author), USDA ARS, Rangeland Resources Res Unit, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. EM Justin.Reeves@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service Climate Change, Soils & Emissions Program; United States Department of Agriculture-Cooperative State Research, Education, and Extension Service Soil Processes Program [2008-35107-18655]; United States Department of Energy's Office of Science (Biological and Environmental Research) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University; United States National Science Foundation (DEB) [1021559] FX Julie Bushey, Nicole McDaniel, Julia Pendergrass, and Sam Williams are thanked for assistance harvesting and processing the plants and data used in this experiment. Dan LeCain, Jack Morgan, Elise Pendall, David Smith and David Williams are thanked for their roles in designing, building and maintaining the PHACE experiment. This work was supported by the United States Department of Agriculture-Agricultural Research Service Climate Change, Soils & Emissions Program, the United States Department of Agriculture-Cooperative State Research, Education, and Extension Service Soil Processes Program (Grant No. 2008-35107-18655), the United States Department of Energy's Office of Science (Biological and Environmental Research) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University, and the United States National Science Foundation (DEB No. 1021559). NR 58 TC 0 Z9 0 U1 5 U2 36 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2015 VL 84 BP 36 EP 43 DI 10.1016/j.biocontrol.2015.02.005 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA CF4HI UT WOS:000352509000005 ER PT J AU Rattner, BA Horak, KE Lazarus, RS Schultz, SL Knowles, S Abbo, BG Volker, SF AF Rattner, Barnett A. Horak, Katherine E. Lazarus, Rebecca S. Schultz, Sandra L. Knowles, Susan Abbo, Benjamin G. Volker, Steven F. TI Toxicity reference values for chlorophacinone and their application for assessing anticoagulant rodenticide risk to raptors SO ECOTOXICOLOGY LA English DT Article DE American kestrel; Anticoagulant rodenticide; Birds; Clotting time; Non-target effects; Risk assessment; Secondary poisoning ID CAPTIVE AMERICAN KESTRELS; OWLS MEGASCOPS-ASIO; NEW-YORK; WILDLIFE; RATS; DIPHACINONE; EXPOSURE; BIRDS; BROMADIOLONE; INTERVALS AB Despite widespread use and benefit, there are growing concerns regarding hazards of second-generation anticoagulant rodenticides to non-target wildlife which may result in expanded use of first-generation compounds, including chlorophacinone (CPN). The toxicity of CPN over a 7-day exposure period was investigated in American kestrels (Falco sparverius) fed either rat tissue mechanically-amended with CPN, tissue from rats fed Rozol(A (R)) bait (biologically-incorporated CPN), or control diets (tissue from untreated rats or commercial bird of prey diet) ad libitum. Nominal CPN concentrations in the formulated diets were 0.15, 0.75 and 1.5 A mu g/g food wet weight, and measured concentrations averaged 94 % of target values. Kestrel food consumption was similar among groups and body weight varied by less than 6 %. Overt signs of intoxication, liver CPN residues, and changes in prothrombin time (PT), Russell's viper venom time (RVVT) and hematocrit, were generally dose-dependent. Histological evidence of hemorrhage was present at all CPN dose levels, and most frequently observed in pectoral muscle and heart. There were no apparent differences in toxicity between mechanically-amended and biologically-incorporated CPN diet formulations. Dietary-based toxicity reference values at which clotting times were prolonged in 50 % of the kestrels were 79.2 A mu g CPN consumed/kg body weight-day for PT and 39.1 A mu g/kg body weight-day for RVVT. Based upon daily food consumption of kestrels and previously reported CPN concentrations found in small mammals following field baiting trials, these toxicity reference values might be exceeded by free-ranging raptors consuming such exposed prey. Tissue-based toxicity reference values for coagulopathy in 50 % of exposed birds were 0.107 A mu g CPN/g liver wet weight for PT and 0.076 A mu g/g liver for RVVT, and are below the range of residue levels reported in raptor mortality incidents attributed to CPN exposure. Sublethal responses associated with exposure to environmentally realistic concentrations of CPN could compromise survival of free-ranging raptors, and should be considered in weighing the costs and benefits of anticoagulant rodenticide use in pest control and eradication programs. C1 [Rattner, Barnett A.; Lazarus, Rebecca S.; Schultz, Sandra L.] US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville, MD 20705 USA. [Horak, Katherine E.; Abbo, Benjamin G.; Volker, Steven F.] US Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Knowles, Susan] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA. RP Rattner, BA (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, BARC East Bldg 308,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM brattner@usgs.gov OI Knowles, Susan/0000-0002-0254-6491 FU U.S. Department of Agriculture; U.S. Geological Survey; California Department of Food and Agriculture Vertebrate Pest Control Research Advisory Committee [11-0430-SA] FX The authors acknowledge W.C. Bauer, D.A. Goldade, J. Green, P.F.P. Henry, C.S. Hulse, N.K. Karouna-Renier, M.M. Maxey, and D.T. Sprague for assistance with the conduct of this study, and thank N.B. Vyas for reviewing a draft of this manuscript. A preliminary report describing dietary-based toxicity reference values for chlorophacinone was presented at the 26th Vertebrate Pest Conference and is described in the proceedings of that meeting. This work was supported by the U.S. Department of Agriculture, U.S. Geological Survey and a grant from the California Department of Food and Agriculture Vertebrate Pest Control Research Advisory Committee (agreement 11-0430-SA). Any use of trade, product or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 51 TC 3 Z9 3 U1 4 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0963-9292 EI 1573-3017 J9 ECOTOXICOLOGY JI Ecotoxicology PD MAY PY 2015 VL 24 IS 4 BP 720 EP 734 DI 10.1007/s10646-015-1418-8 PG 15 WC Ecology; Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA CF3II UT WOS:000352441500002 PM 25600128 ER PT J AU Alcasena, FJ Salis, M Ager, AA Arca, B Molina, D Spano, D AF Alcasena, Fermin J. Salis, Michele Ager, Alan A. Arca, Bachisio Molina, Domingo Spano, Donatella TI Assessing Landscape Scale Wildfire Exposure for Highly Valued Resources in a Mediterranean Area SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Fire exposure; Fire risk; Highly valued resources and assets; Mediterranean areas; MTT algorithm ID WILDLAND-URBAN INTERFACE; FIRE MANAGEMENT; FUEL TREATMENTS; UNITED-STATES; RISK ANALYSIS; FOREST-FIRES; SPATIAL-PATTERNS; SURFACE WINDS; MEGA-FIRES; SPAIN AB We used a fire simulation modeling approach to assess landscape scale wildfire exposure for highly valued resources and assets (HVR) on a fire-prone area of 680 km(2) located in central Sardinia, Italy. The study area was affected by several wildfires in the last half century: some large and intense fire events threatened wildland urban interfaces as well as other socioeconomic and cultural values. Historical wildfire and weather data were used to inform wildfire simulations, which were based on the minimum travel time algorithm as implemented in FlamMap. We simulated 90,000 fires that replicated recent large fire events in the area spreading under severe weather conditions to generate detailed maps of wildfire likelihood and intensity. Then, we linked fire modeling outputs to a geospatial risk assessment framework focusing on buffer areas around HVR. The results highlighted a large variation in burn probability and fire intensity in the vicinity of HVRs, and allowed us to identify the areas most exposed to wildfires and thus to a higher potential damage. Fire intensity in the HVR buffers was mainly related to fuel types, while wind direction, topographic features, and historically based ignition pattern were the key factors affecting fire likelihood. The methodology presented in this work can have numerous applications, in the study area and elsewhere, particularly to address and inform fire risk management, landscape planning and people safety on the vicinity of HVRs. C1 [Alcasena, Fermin J.] Univ Lleida, Dept Agr & Forest Engn, Lleida 25198, Spain. [Alcasena, Fermin J.; Salis, Michele; Spano, Donatella] Ctr Climate Change CMCC, IAFENT Div, Euromediterranean, I-07100 Sassari, Italy. [Salis, Michele; Spano, Donatella] Univ Sassari, Dept Sci Nat & Environm Resources DIPNET, I-07100 Sassari, Italy. [Ager, Alan A.] US Forest Serv, Pacific NW Res Stn, USDA, Pendleton, OR 97801 USA. [Arca, Bachisio] Natl Res Council CNR, Inst Biometeorol IBIMET, I-07100 Sassari, Italy. [Molina, Domingo] Univ Lleida, Dept Crop & Forest Sci, Lleida 25198, Spain. RP Alcasena, FJ (reprint author), Univ Lleida, Dept Agr & Forest Engn, Ave Rovira Roure 191, Lleida 25198, Spain. EM ferminalcasena@eagrof.udl.cat OI Alcasena Urdiroz, Fermin Jesus/0000-0002-2007-9487 FU GEMINA Project - MIUR/MATTM [232/2011]; EXTREME Project (Legge Regione Sardegna) [CRP-25405]; Project "Modeling approach to evaluate fire risk and mitigation planning actions'' (P.O.R. SARDEGNA F.S.E., Asse IV Capitale umano, Linea di Attivita' l.3.1) FX The authors would like to thank the Forest Service of Sardinia and the Sardinia Civil Protection for collaborating in this study. This work was partially funded by the GEMINA Project - MIUR/MATTM n. 232/2011, by the EXTREME Project (Legge Regione Sardegna 7/2007, CRP-25405), and by the Project "Modeling approach to evaluate fire risk and mitigation planning actions'' (P.O.R. SARDEGNA F.S.E. 2007-2013, Asse IV Capitale umano, Linea di Attivita' l.3.1). NR 101 TC 11 Z9 11 U1 2 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD MAY PY 2015 VL 55 IS 5 BP 1200 EP 1216 DI 10.1007/s00267-015-0448-6 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA CF6CZ UT WOS:000352645600017 PM 25613434 ER PT J AU Stone, KC Bauer, PJ Busscher, WJ Millen, JA Evans, DE Strickland, EE AF Stone, K. C. Bauer, P. J. Busscher, W. J. Millen, J. A. Evans, D. E. Strickland, E. E. TI Variable-rate irrigation management using an expert system in the eastern coastal plain SO IRRIGATION SCIENCE LA English DT Article AB Variable-rate irrigation (VRI) systems have the potential to conserve water by spatially allocating limited water resources. However, when compared to traditional irrigation systems, VRI systems require a higher level of management. In this 3-year study, we evaluated spatial irrigation management of a peanut crop grown under a VRI system using an expert system (Irrigator Pro). The irrigation management treatments evaluated were: (1) using Irrigator Pro (IP) to manage irrigation uniformly in plots with varying soils; (2) using Irrigator Pro to manage irrigation in plots based on the individual soils (IPS); (3) a treatment based on maintaining soil water potential (SWP) above -30 kPa (approximately 50 % depletion of available water) in the surface 30 cm of each soil within a plot; and (4) a non-irrigated treatment. Over the 3-year study, all irrigated treatments had significantly higher yields (4,230, 4,130, and 4,394 kg ha(-1) for the IP, IPS, and SWP treatments, respectively) than the non-irrigated treatment (3,285 kg ha(-1)), yet the yields of the three irrigation treatments were not significantly different. Averaged over the 3-year experiment, the three treatments did not differ significantly in water usage. In the 2007 and 2009 growing seasons with below normal rainfall, the IP and IPS treatments required significantly greater total water than the SWP treatment. Overall, water use efficiency was significantly higher for the non-irrigated and SWP treatments (9.4 and 8.9 kg ha(-1) per mm, respectively). The lower water use efficiency for the IP and IPS irrigation treatments (7.8 kg ha(-1) per mm) was attributed to greater water applications mainly due to earlier growing season initiation of irrigation applications. However, the IP and IPS treatments maintained soil water potentials at the 30- and 60-cm depths at higher levels throughout most of the season. The two Irrigator Pro expert system treatments functioned as well as the SWP-based treatment. The Irrigator Pro expert system can be effectively used for site-specific management where management zone soils do not greatly differ. Further refinement of the expert system may be needed to improve its application in spatial irrigation applications. C1 [Stone, K. C.; Bauer, P. J.; Busscher, W. J.; Millen, J. A.; Evans, D. E.; Strickland, E. E.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Stone, KC (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. EM ken.stone@ars.usda.gov NR 19 TC 2 Z9 3 U1 1 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAY PY 2015 VL 33 IS 3 BP 167 EP 175 DI 10.1007/s00271-014-0457-x PG 9 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CF8HV UT WOS:000352797900001 ER PT J AU Yee, WL AF Yee, W. L. TI Commercial Yellow Sticky Strips more attractive than yellow boards to western cherry fruit fly (Dipt., Tephritidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE non-target insects; Rhagoletis indifferens; translucent traps ID RHAGOLETIS-INDIFFERENS DIPTERA; APPLE MAGGOT FLIES; RECTANGLE TRAPS; DACUS-OLEAE; RED SPHERES; OLIVE FLY; CERASI L; PHOTOTAXIS; CAPTURES; COLOR AB Bright yellow sticky rectangles made of paper boards were previously identified as the most effective traps for capturing western cherry fruit fly, Rhagoletis indifferens Curran (Dipt., Tephritidae). However, no data on the effectiveness of commercial sticky yellow plastic traps against R.indifferens have been reported. In tests conducted in sweet cherry trees [Prunus avium (L.) L.] in Washington state (USA) using ammonium carbonate as the chemical lure, commercial plastic Yellow Sticky Strips' made of translucent high-impact polystyrene captured two or three times more flies than commercial sticky yellow-folded Pherocon((R)) AM and Alpha Scents boards. Yellow Sticky Strips also minimized captures of non-target flies and bees per surface area compared with Pherocon((R))AM and/or Alpha Scents boards. Trap size and adhesive type were not factors for greater catches of R.indifferens. However, more flies were caught on the shade-facing side of Yellow Sticky Strips, which was brightly illuminated, than on the shade-facing side of boards, which was darker, suggesting differential light passage was a factor. The Yellow Sticky Strips could be very useful for monitoring R.indifferens in detection programmes and based on the results of this study can replace yellow boards. They are also useful because they are relatively unattractive to non-target insects. C1 USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Yee, WL (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov NR 42 TC 1 Z9 1 U1 2 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD MAY PY 2015 VL 139 IS 4 BP 289 EP 301 DI 10.1111/jen.12157 PG 13 WC Entomology SC Entomology GA CF7HA UT WOS:000352725800006 ER PT J AU Stewart, JAE Perrine, JD Nichols, LB Thorne, JH Millar, CI Goehring, KE Massing, CP Wright, DH AF Stewart, Joseph A. E. Perrine, John D. Nichols, Lyle B. Thorne, James H. Millar, Constance I. Goehring, Kenneth E. Massing, Cody P. Wright, David H. TI Revisiting the past to foretell the future: summer temperature and habitat area predict pika extirpations in California SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE Climate change; global warming; historical resurvey; metapopulation; Ochotona princeps; pika; range shift; species distribution modelling; talus ID CLIMATE ENVELOPE MODELS; OCHOTONA-PRINCEPS; AMERICAN PIKA; GREAT-BASIN; SIERRA-NEVADA; SPECIES DISTRIBUTIONS; GLOBAL CHANGE; THERMOREGULATION; TRANSFERABILITY; BIODIVERSITY AB Aim The American pika (Ochotona princeps) appears to have experienced climate-mediated upslope range contraction in the Great Basin of North America, but this result has not yet been extended to other portions of the pika's range. Our goals were: first, to determine the environmental parameters that most influence current pika distribution within California; second, to infer whether these constraints explain extirpations that have occurred in California; third, to predict future extirpations; and fourth, to advance methods for assessing the degree to which pikas and other climate-sensitive mammals are threatened by climate change. Location Historical pika record locations in California, USA, spanning four degrees of latitude and longitude, from Mount Shasta to the southern Sierra Nevada. Methods We identified 67 precise historical pika record locations and surveyed them exhaustively, over multiple years, to determine whether pika populations persist at those sites. We used an information theoretic approach and logistic regression to model current pika occupancy as a function of 16 environmental variables, tested our best-performing model as a predictor of historical occupancy, and then used our model to predict future pika occupancy given anticipated climate change. Results Pikas no longer occurred at 10 of 67 (15%) historical sites in California. The best predictors of occupancy were average summer temperature and talus habitat area within a 1-km radius. A logistic model fitted to this relationship correctly predicted current occupancy at 94% of sites and correctly hindcasted past occupancy at 93% of sites, suggesting that the model has strong temporal transferability. Depending on the future climate scenario, our model projects that by 2070 pikas will be extirpated from 39% to 88% of these historical sites in California. Main conclusions Our simple species distribution model for pikas performs remarkably well for both current and historical periods. Pika distribution appears to be governed primarily by behavioural restrictions mediated by summer temperature and by the configuration of talus habitat available to pikas locally. Pikas, and other montane species in the western USA, may be subjected to above-average exposure to climate change because summer temperature is projected to rise more than annual temperature. C1 [Stewart, Joseph A. E.] Univ Calif Santa Cruz, Dept Ecol & Evolutionary Biol, Santa Cruz, CA 95064 USA. [Stewart, Joseph A. E.; Wright, David H.] Calif Dept Fish & Wildlife, Rancho Cordova, CA 95670 USA. [Perrine, John D.; Massing, Cody P.] Calif Polytech State Univ San Luis Obispo, Dept Biol Sci, San Luis Obispo, CA 93405 USA. [Nichols, Lyle B.] Santa Monica Coll, Dept Life Sci, Santa Monica, CA 90405 USA. [Thorne, James H.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Millar, Constance I.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Goehring, Kenneth E.] Coll Siskiyous, Dept Biol, Weed, CA 96094 USA. RP Stewart, JAE (reprint author), Univ Calif Santa Cruz, Earth & Marine Sci Bldg,1156 High St, Santa Cruz, CA 95064 USA. EM jaes@ucsc.edu FU US Fish and Wildlife Service, State Wildlife Grant FX Access to museum records and specimens was facilitated by C. Conroy and J. Patton, University of California Berkeley Museum of Vertebrate Zoology; I. Engilis, University of California Davis Museum of Wildlife and Fish Biology; and D. Castronovo, California Academy of Sciences. Additional historical sites were identified through correspondence with A. Smith, C. Ray and J. Patton. We thank many field assistants - J. Boulat, K. Graham, J. N. Henderson, S. Huskins, K. Leviste, C. Nguyen, P. Nichols, R. Pauloo, T. Rosenthal, S. Stewart, K. Wiggins and M. Wilder. Funding for north-central California surveys was provided by a US Fish and Wildlife Service, State Wildlife Grant to the California Department of Fish and Wildlife. NR 59 TC 15 Z9 16 U1 9 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD MAY PY 2015 VL 42 IS 5 BP 880 EP 890 DI 10.1111/jbi.12466 PG 11 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CF8GH UT WOS:000352793700007 ER PT J AU Young, JK Mahe, M Breck, S AF Young, Julie K. Mahe, Marion Breck, Stewart TI Evaluating behavioral syndromes in coyotes (Canis latrans) SO JOURNAL OF ETHOLOGY LA English DT Article DE Aggression; Canis latrans; Flight-initiation distance; Novel object; Personality ID GUPPIES POECILIA-RETICULATA; FLIGHT-INITIATION DISTANCE; HUMAN DISTURBANCE; SOCIAL-ORGANIZATION; ANIMAL PERSONALITY; LIFE-HISTORY; SPACE USE; EVOLUTIONARY; RESPONSES; STIMULI AB As more research focuses on behavioral syndromes and their role in ecological and evolutionary processes, it is imperative that methods to test behavior are valid. The objectives of this study were to (1) assess behavior in captive coyotes (Canis latrans) using three methods [agitation scores, novel object test, and flight-initiation distance (FID)] and (2) to determine whether the three tests were correlated within individuals to describe behavioral syndromes. Female coyotes had higher agitation scores during handling (2.6 +/- A 0.5) than males (1.5 +/- A 0.3; t = 1.90, p = 0.06): scores ranged from 0 to 8 on a 0-11 scale. The most common behavior observed was biting at y-stick. Only 27 % of males and 10 % of females approached a novel object within 1 m, with females (37.3 +/- A 18.6 s) taking less time than males (136.7 +/- A 50.4 s; p = 0.09). There was no difference in the distance at which males (17.5 +/- A 4.0 m) and females fled during FID tests (20.7 +/- A 5.4 m; p = 0.64, n = 30). We found no relationships between FID and agitation scores (r (2) = 0.13, p = 0.12) or time to approach a novel object and agitation scores (r (2) = 0.001, p = 0.89). There was a slightly positive relationship between FID and time to approach a novel object (r (2) = 0.15, p = 0.03), but no relationship among all three tests (r (2) = 0.15, p = 0.45). Our results suggest a behavioral syndrome for boldness and explorations, but these traits are unlikely to be coupled with aggression in coyotes. While these three tests may not be ideally combined to create a behavioral syndrome in individual coyotes, using FID and novel object testing may elucidate a type of behavioral syndrome. C1 [Young, Julie K.] Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Predator Res Facil,Dept Wil, Logan, UT 84322 USA. [Mahe, Marion] Agrocampus Ouest Rennes, Dept Agron, F-35042 Rennes, France. [Breck, Stewart] USDA, Wildlife Serv, Natl Wildlife Res Ctr, Predator Res Facil, Ft Collins, CO 80521 USA. RP Young, JK (reprint author), Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Predator Res Facil,Dept Wil, BNR 163, Logan, UT 84322 USA. EM julie.young@usu.edu; mmahe@agrocampus-ouest.fr; stewart.w.breck@aphis.usda.gov FU USDA-Wildlife Services-National Wildlife Research Center FX Stacey Brummer and Stan Gehrt assisted with project design. We thank Stacey Brummer and Delphine Napoletano for field assistance. We are especially thankful to the NWRC staff and many volunteers that assisted with coyote captures. Heath Weaver was helpful in reviewing the manuscript. This project was funded by USDA-Wildlife Services-National Wildlife Research Center. Funding had no role in study design, collection, analysis and interpretation of data, writing, or the decision to submit the article for publication. NR 63 TC 3 Z9 3 U1 9 U2 35 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 0289-0771 EI 1439-5444 J9 J ETHOL JI J. Ethol. PD MAY PY 2015 VL 33 IS 2 BP 137 EP 144 DI 10.1007/s10164-015-0422-z PG 8 WC Behavioral Sciences; Zoology SC Behavioral Sciences; Zoology GA CF9CX UT WOS:000352860300006 ER PT J AU Lupoli, CA Morse, WC Bailey, C Schelhas, J AF Lupoli, Christopher A. Morse, Wayde C. Bailey, Conner Schelhas, John TI Indicator development methodology for volunteer tourism in host communities: creating a low-cost, locally applicable, rapid assessment tool SO JOURNAL OF SUSTAINABLE TOURISM LA English DT Article DE volunteer tourism; indicators; participatory planning; tourism impacts; community participation ID SUSTAINABILITY INDICATORS; PERSPECTIVE; WORLD AB Two prominent critiques of volunteer tourism are that it is a top-down imposed form of development treating host communities as passive recipients of international aid, and that the impacts of volunteer tourism in host communities are not systematically evaluated. To address this we identified a pre-existing participatory methodology for assessing community sustainability (the compass of sustainability) and adapted it as a rapid low-cost indicator tool for volunteer tourism impact evaluation. We created and tested a development methodology that could be applied through local community workshops by local people and sending organizations within each unique host community, and repeated over time. Testing took place in five contrasting communities hosting volunteer tourism in Ecuador and Costa Rica. Each workshop generated and organized numerous indicators of community welfare, categorized into nature, economy, society and personal well-being. Interrelations were identified among the indicators to promote a systemic understanding of community well-being. Indicators were prioritized and strategies for measuring impacts were discussed to encourage the establishment of accomplishable goals. Evaluation of the compass method as a tool for community participation in indicator development is discussed as a potential facilitator for local voices and the construction of third spaces in volunteer tourism. C1 [Lupoli, Christopher A.; Morse, Wayde C.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Bailey, Conner] Auburn Univ, Dept Agr Econ & Rural Sociol, Auburn, AL 36849 USA. [Schelhas, John] US Forest Serv, USDA, Athens, GA USA. RP Lupoli, CA (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. EM cal0022@auburn.edu NR 57 TC 0 Z9 0 U1 4 U2 21 PU CHANNEL VIEW PUBLICATIONS PI CLEVEDON PA FRANKFURT LODGE, CLEVEDON HALL, VICTORIA ROAD, CLEVEDON, BS21 7HH, ENGLAND SN 0966-9582 EI 1747-7646 J9 J SUSTAIN TOUR JI J. Sustain. Tour. PD MAY PY 2015 VL 23 IS 5 BP 726 EP 747 DI 10.1080/09669582.2015.1008498 PG 22 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Hospitality, Leisure, Sport & Tourism SC Science & Technology - Other Topics; Social Sciences - Other Topics GA CF5GC UT WOS:000352584200005 ER PT J AU Palti, Y Gao, G Liu, S Kent, MP Lien, S Miller, MR Rexroad, CE Moen, T AF Palti, Y. Gao, G. Liu, S. Kent, M. P. Lien, S. Miller, M. R. Rexroad, C. E., III Moen, T. TI The development and characterization of a 57K single nucleotide polymorphism array for rainbow trout SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE genetic diversity; linkage analysis; rainbow trout; single nucleotide polymorphism chip ID TILAPIA OREOCHROMIS-AUREUS; WESTSLOPE CUTTHROAT TROUT; SNP GENOTYPING ASSAY; SALMON SALMO-SALAR; ONCORHYNCHUS-MYKISS; ATLANTIC SALMON; CHROMOSOME ARMS; INTEGRATED MAP; INBRED LINE; O. MYKISS AB In this study, we describe the development and characterization of the first high-density single nucleotide polymorphism (SNP) genotyping array for rainbow trout. The SNP array is publically available from a commercial vendor (Affymetrix). The SNP genotyping quality was high, and validation rate was close to 90%. This is comparable to other farm animals and is much higher than previous smaller scale SNP validation studies in rainbow trout. High quality and integrity of the genotypes are evident from sample reproducibility and from nearly 100% agreement in genotyping results from other methods. The array is very useful for rainbow trout aquaculture populations with more than 40900 polymorphic markers per population. For wild populations that were confounded by a smaller sample size, the number of polymorphic markers was between 10577 and 24330. Comparison between genotypes from individual populations suggests good potential for identifying candidate markers for populations' traceability. Linkage analysis and mapping of the SNPs to the reference genome assembly provide strong evidence for a wide distribution throughout the genome with good representation in all 29 chromosomes. A total of 68% of the genome scaffolds and contigs were anchored through linkage analysis using the SNP array genotypes, including 20% of the genome assembly that has not been previously anchored to chromosomes. C1 [Palti, Y.; Gao, G.; Liu, S.; Rexroad, C. E., III] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Kent, M. P.; Lien, S.] Norwegian Univ Life Sci, Ctr Integrat Genet CIGENE, N-1432 As, Norway. [Kent, M. P.; Lien, S.] Norwegian Univ Life Sci, Dept Anim & Aquacultural Sci, N-1432 As, Norway. [Miller, M. R.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Moen, T.] AquaGen AS, N-7462 Trondheim, Norway. RP Palti, Y (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM yniv.palti@ars.usda.gov OI Palti, Yniv/0000-0002-4953-8878 FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-67015-30091] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30091 from the USDA National Institute of Food and Agriculture. The SNP chip was developed in cooperation with Affymetrix, Inc., and we particularly thank the following Affymetrix personnel for their direct contribution: LuAnn Glaser, Fiona Brew, Michael Mittmann, Lakshmi Radhakrishnan, Eric Schell and Ali Pirani. We thank Roseanna Long and Kristy Shewbridge for their technical assistance and Beth Cleveland for the modified sex determination PCR protocol and reagents. Tissue or DNA samples for rainbow trout populations' survey were contributed by Kyle Martin, Troutlodge Inc.; Scott LaPatra and Richard Towner, Clear Springs Food; Melinda Baerwald and Molly Stephens, UC Davis; Edwige Quillet, INRA, France; Krista Nichols and Matthew Hale, Purdue University; and Ken Overturf, USDA-ARS, Hagerman, Idaho. Samples of DH lines were contributed by Paul Wheeler and Gary Thorgaard, Washington State University and by Edwige Quillet, INRA. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 37 TC 23 Z9 23 U1 8 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAY PY 2015 VL 15 IS 3 BP 662 EP 672 DI 10.1111/1755-0998.12337 PG 11 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CF6FY UT WOS:000352653700018 PM 25294387 ER PT J AU Zhu, QB He, CL Lu, RF Mendoza, F Cen, HY AF Zhu, Qibing He, Chunliu Lu, Renfu Mendoza, Fernando Cen, Haiyan TI Ripeness evaluation of 'Sun Bright' tomato using optical absorption and scattering properties SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Tomato; Ripeness; Optical properties; Absorption; Scattering; Hyperspectral imaging ID NEAR-INFRARED SPECTROSCOPY; NIR SPECTROSCOPY; TURBID MATERIALS; PROCESSING TOMATOES; LYCOPENE CONTENT; RAPID ASSESSMENT; GREEN TOMATOES; IMAGE-ANALYSIS; HAND-HELD; MATURITY AB Ripeness is one of the most important factors in determining the processing and eating quality of tomato. The objective of this research was to evaluate the changes of optical absorption and scattering properties in tomatoes during ripening, and develop classification models for grading the ripeness of tomatoes using optical absorption and scattering spectra. Optical absorption (mu(a)) and reduced scattering (mu(s)') coefficients over the spectral region between 500 nm and 950 nm were measured, using a hyperspectral imaging-based spatially-resolved instrument, for 281 'Sun Bright' tomatoes harvested at six ripeness grades (i.e., 'Green', 'Breaker', 'Turning', 'Pink', 'Light-red', 'Red'). Absorption peak around 675 nm decreased consistently with the progression of ripeness, and its mean value for 'Red' tomatoes was almost zero. The reduced scattering spectra, on the other hand, decreased monotonically with the increasing wavelength; the value of mu(s): also decreased successively from 'Green' to 'Turning', and an opposite trend was observed from 'Pink' to 'Red'. Partial least squares discriminant analysis (PLS-DA) models yielded 92.1%, 84.4%, 92.3%, and 92.1% classification accuracies for the three ripeness grades (i.e., 'Green/Breaker', 'Turning/Pink', and 'Light-red/Red'), when using the full spectra (500-950 nm) of mu(a), mu(s), mu(a)&&MU;(s) and the effective attenuation coefficient (mu(eff) = [3 mu(a)(mu(a)+mu(s)')](1/2)), respectively. The PLS-DA model using mu(eff) achieved an overall classification accuracy of 88.4% for the six ripeness grades, which was 2.5% and 10.2% better than using the optical parameters of mu(a), and mu(s)' alone. The research demonstrated that the optical absorption and scattering spectra, especially their combinations, are effective for classification of tomato ripeness. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhu, Qibing; He, Chunliu] Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China. [Lu, Renfu] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Mendoza, Fernando] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Cen, Haiyan] Michigan State Univ, Dept Food Sci & Human Nutr, E Lansing, MI 48824 USA. RP Zhu, QB (reprint author), Jiangnan Univ, Key Lab Adv Proc Control Light Ind, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China. FU National Natural Science Foundation of China [61275155, 61271384]; 111 Project [1312018] FX Dr. Qibing Zhu and Miss. Chunliu He gratefully acknowledge financial support from the National Natural Science Foundation of China (Grant nos. 61275155 and 61271384) and the 111 Project (1312018). NR 34 TC 7 Z9 8 U1 5 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAY PY 2015 VL 103 BP 27 EP 34 DI 10.1016/j.postharvbio.2015.02.007 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA CG2EU UT WOS:000353088500004 ER PT J AU Hua, SST Hernlem, BJ Yokoyama, W Sarreal, SBL AF Hua, Sui Sheng T. Hernlem, Bradley J. Yokoyama, Wallace Sarreal, Siov Bouy L. TI Intracellular trehalose and sorbitol synergistically promoting cell viability of a biocontrol yeast, Pichia anomala, for aflatoxin reduction SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Wickerhamomyces anomalus; Pichia anomala; Biocontrol; Shelf-life; Sorbitol; Trehalose; Cell viability; Fluorescent stains; Aflatoxin ID ASPERGILLUS-FLAVUS; WICKERHAMOMYCES-ANOMALUS; GROWTH; WHEAT AB Pichia anomala (Wickerhamomyces anomalus) WRL-076 was discovered by a visual screening bioassay for its antagonism against Aspergillus flavus. The yeast was shown to significantly inhibit aflatoxin production and the growth of A. flavus. P. anomala is a potential biocontrol agent for reduction of aflatoxin in the food chain. Maintaining the viability of biocontrol agents in formulated products is a great challenge for commercial applications. Four media, NYG, NYGS, NYGT and NYGST are described which support good growth of yeast cells and were tested as storage formulations. Post growth supplement of 5 % trehalose to NYGST resulted in 83 % viable yeast cells after 12 months in cold storage. Intracellular sorbitol and trehalose concentrations were determined by HPLC analysis at the beginning of the storage and at the end of 12 month. Correlation of cell viability to both trehalose and sorbitol suggested a synergistic effect. Bonferroni (Dunn) t Test, Tukey's Studentized Range (HSD) Test and Duncan's Multiple Range Test, all showed that yeast cell viability in samples with both intracellular trehalose and sorbitol were significantly higher than those with either or none, at a 95 % confidence level. DiBAC(4)(5) and CFDA-AM were used as the membrane integrity fluorescent stains to create a two-color vital staining scheme with red and green fluorescence, respectively. Yeast cells stored in formulations NYG and NYGS with no detectable trehalose, displayed mostly red fluorescence. Yeast cells in NYGST+5T showed mostly green fluorescence. C1 [Hua, Sui Sheng T.; Hernlem, Bradley J.; Yokoyama, Wallace; Sarreal, Siov Bouy L.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Hua, SST (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM sylvia.hua@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Services [5325-42000-038, 5325-42000-048, 5325-41440-006] FX This work is supported by in-house research program funds from U.S. Department of Agriculture, Agricultural Research Services (Project Numbers: 5325-42000-038, 5325-42000-048 and 5325-41440-006. We thank Thomas McKeon and Yuzhu Zhang for comments; Melissa Sanchez for technical assistance. NR 21 TC 2 Z9 2 U1 3 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD MAY PY 2015 VL 31 IS 5 BP 729 EP 734 DI 10.1007/s11274-015-1824-3 PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CF6EI UT WOS:000352649200005 PM 25700743 ER PT J AU Sindelar, AJ Coulter, JA Lamb, JA Vetsch, JA AF Sindelar, Aaron J. Coulter, Jeffrey A. Lamb, John A. Vetsch, Jeffrey A. TI Nitrogen, Stover, and Tillage Management Affect Nitrogen Use Efficiency in Continuous Corn SO AGRONOMY JOURNAL LA English DT Article ID SOIL ORGANIC-CARBON; CROP RESIDUE; SOYBEAN ROTATION; IRRIGATED CORN; TILE DRAINAGE; MAIZE; FERTILIZATION; NITRATE; SYSTEMS; YIELD AB Improving nitrogen use effciency (NUE) in corn (Zea mays L.) is critical for optimizing yield and reducing environmental impact. Stover removal in continuous corn (CC) for biofuel production, coupled with reduced-tillage systems, could alter NUE and residual soil nitrate-N. Experiments were conducted in Minnesota over 3 yr to determine how N uptake, NUE, and residual soil nitrate-N are affected by stover (remove and retain), tillage (chisel-[CT], strip-[ST], and no-till [NT]), and fertilizer N (0, 45, 89, 134, 179, and 224 kg N ha(-1)) management. There was a linear response of grain and total aboveground N uptake to fertilizer N across stover management and tillage treatments. Stover removal increased recovery effciency for ST and NT at fertilizer N rates <= 134 kg N ha(-1), but did not enhance N recovery in plots fertilized at rates > 134 kg N ha(-1). Internal efficiency was 5% greater with stover removal with no fertilizer N, but did not change with fertilizer N application. Stover removal did not affect agronomic efficiency in CT but did enhance it in ST and NT at fertilizer N rates <= 134 kg N ha(-1). Residual soil nitrate-N to a depth of 1.2 m was 10 to 16 kg NO3-N ha(-1) greater with stover removal at fertilizer N rates of 134 to 224 kg N ha(-1). Greater residual soil nitrate-N with stover removal indicates a need for judicious management of N when stover is removed in intensively-managed Upper Midwestern CC systems. C1 [Sindelar, Aaron J.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Coulter, Jeffrey A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Lamb, John A.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Vetsch, Jeffrey A.] Univ Minnesota, Southern Res & Outreach Ctr, Waseca, MN 56093 USA. RP Sindelar, AJ (reprint author), Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Rm 118,Keim Hall,East Campus, Lincoln, NE 68583 USA. EM aaron.sindelar@ars.usda.gov FU Minnesota Corn Growers Research and Promotion Council FX Funding for this study was provided by the Minnesota Corn Growers Research and Promotion Council. The use of trade or commercial product names in this publication is solely for informational purposes and does not imply recommendation, endorsement, or approval by the USDA. The USDA is an equal opportunity employer and provider. NR 58 TC 4 Z9 4 U1 0 U2 0 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2015 VL 107 IS 3 BP 843 EP 850 DI 10.2134/agronj14.0535 PG 8 WC Agronomy SC Agriculture GA CH6YN UT WOS:000354182600003 ER PT J AU Hughes, H Olson, Z Gauger, P Vincent, A Brockmeier, S Loving, C AF Hughes, Holly Olson, Zahra Gauger, Phillip Vincent, Amy Brockmeier, Susan Loving, Crystal TI Immune mechanisms associated with enhanced influenza A virus disease versus cross-protection in vaccinated pigs. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (IMMUNOLOGY) CY MAY 08-12, 2015 CL New Orleans, LA SP Amer Assoc Immunologists C1 [Gauger, Phillip] Iowa State Univ, Vet Diagnost & Prod Anim Med, Ames, IA USA. [Hughes, Holly; Olson, Zahra; Vincent, Amy; Brockmeier, Susan; Loving, Crystal] USDA ARS, Natl Anim Dis Ctr, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2015 VL 194 SU 1 MA VAC4P.1064 PG 1 WC Immunology SC Immunology GA DQ7RG UT WOS:000379404504127 ER PT J AU Makkar, S Liyanage, R Lay, J Narayan, R AF Makkar, Sarbjeet Liyanage, Rohana Lay, Jackson Narayan, Rath TI Proteomic analysis of macrophages activated with salmonella lipopolysaccharide SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (IMMUNOLOGY) CY MAY 08-12, 2015 CL New Orleans, LA SP Amer Assoc Immunologists C1 [Makkar, Sarbjeet] Univ Arkansas, Poultry Sci, Fayetteville, AR 72701 USA. [Makkar, Sarbjeet; Narayan, Rath] ARS, USDA, PPRSU, Fayetteville, AR USA. [Liyanage, Rohana; Lay, Jackson] Univ Arkansas, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2015 VL 194 SU 1 MA INC2P.413 PG 1 WC Immunology SC Immunology GA DQ7RG UT WOS:000379404502087 ER PT J AU Palaniyandi, S Liu, XY Periasamy, S Ma, AY Tang, J Jenkins, M Tuo, WB Song, WX Keegan, A Conrad, D Zhu, XP AF Palaniyandi, Senthilkumar Liu, Xiaoyang Periasamy, Sivakumar Ma, Aiying Tang, Jin Jenkins, Mark Tuo, Wenbin Song, Wenxia Keegan, Achsah Conrad, Daniel Zhu, Xiaoping TI Inhibition of CD23-mediated IgE transcytosis suppresses the initiation and development of airway allergic inflammation SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (IMMUNOLOGY) CY MAY 08-12, 2015 CL New Orleans, LA SP Amer Assoc Immunologists C1 [Palaniyandi, Senthilkumar; Liu, Xiaoyang; Ma, Aiying; Tang, Jin; Song, Wenxia; Keegan, Achsah; Conrad, Daniel; Zhu, Xiaoping] Univ Maryland, College Pk, MD 20742 USA. [Jenkins, Mark; Tuo, Wenbin] USDA, Beltsville, MD 20705 USA. [Periasamy, Sivakumar] Albany Med Coll, Albany, NY 12208 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2015 VL 194 SU 1 MA HYP4P.308 PG 1 WC Immunology SC Immunology GA DQ7RG UT WOS:000379404502265 ER PT J AU Pardington, P Chaudhary, A Norvell, M Gupta, G Dandekar, A Gouran, H Uratsu, S Aguero, C Feldstein, P Bruening, G Civerolo, E Hao, GX Duan, YP Stover, E AF Pardington, Paige Chaudhary, Anu Norvell, Meghan Gupta, Goutam Dandekar, Abhaya Gouran, Hossein Uratsu, Sandra Aguero, Cecilia Feldstein, Paul Bruening, George Civerolo, Edwin Hao, Guixia Duan, YongPing Stover, Ed TI Pathogen clearance by engineering of novel innate immune defense SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (IMMUNOLOGY) CY MAY 08-12, 2015 CL New Orleans, LA SP Amer Assoc Immunologists C1 [Gupta, Goutam] Los Alamos Natl Lab, Los Alamos, NM USA. [Pardington, Paige] Los Alamos Natl Lab, Biosecur & Publ Hlth, Los Alamos, NM USA. [Chaudhary, Anu] Univ Washington, Microbiol, Los Alamos, NM USA. [Norvell, Meghan] NanoMR, Business & Technol Dev, Albuquerque, NM USA. [Dandekar, Abhaya; Uratsu, Sandra; Aguero, Cecilia; Feldstein, Paul; Bruening, George] Univ Calif Davis, Plant Sci, Davis, CA 95616 USA. [Gouran, Hossein] Monsanto Co, Biotechnol, Davis, CA USA. [Civerolo, Edwin] USDA ARS, Plant Pathol, Davis, CA USA. [Hao, Guixia] US Hort Res Lab, Subtrop Insects & Hort, Ft Pierce, FL USA. [Duan, YongPing] US Hort Res Lab, Plant Pathol, Ft Pierce, FL USA. [Stover, Ed] USDA ARS, Hort & Genet, Ft Pierce, FL USA. [Stover, Ed] US Hort Res Lab, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2015 VL 194 SU 1 MA INM3P.414 PG 1 WC Immunology SC Immunology GA DQ7RG UT WOS:000379404503210 ER PT J AU Kurtzman, CP Robnett, CJ AF Kurtzman, Cletus P. Robnett, Christie J. TI Occultifur kilbournensis f.a. sp nov., a new member of the Cystobasidiales associated with maize (Zea mays) cultivation SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Occultifur; Yeasts from maize; New species; Systematics ID YEASTS; CORN AB During a study of microorganisms associated with maize (Zea mays) cultivation, yeasts were isolated from overwintered stalks, cobs and surrounding soil, which were collected from an agricultural field in south-central Illinois, USA. Predominant among isolates were two species of Cryptococcus (Cr. flavescens, Cr. magnus) and a red yeast that D1/D2 LSU rRNA gene sequences revealed to be a new species of the basidiomycete yeast genus Occultifur. The species, which was not detected in the same field during the growing season, is described here as Occultifur kilbournensis (MycoBank number MB 811259; type strain NRRL Y-63695, CBS 13982, GenBank numbers, D1/D2 LSU rRNA gene, KP413160, ITS, KP413162; allotype strain NRRL Y-63699, CBS 13983). Mixture of the type and allotype strains resulted in formation of hyphae with clamp connections and a small number of apparent basidia following incubation on 5 % malt extract agar at 15 A degrees C for 2 months. In view of the uncertainty of the life cycle, the new species is being designated as forma asexualis. From analysis of D1/D2 and ITS nucleotide sequences, the new species is most closely related to Occultifur externus. C1 [Kurtzman, Cletus P.; Robnett, Christie J.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 16 TC 2 Z9 2 U1 2 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD MAY PY 2015 VL 107 IS 5 BP 1323 EP 1329 DI 10.1007/s10482-015-0427-1 PG 7 WC Microbiology SC Microbiology GA CF3GI UT WOS:000352435800022 PM 25761862 ER PT J AU Evans, KO Laszlo, JA Compton, DL AF Evans, Kervin O. Laszlo, Joseph A. Compton, David L. TI Hydroxytyrosol and tyrosol esters partitioning into, location within, and effect on DOPC liposome bilayer behavior SO BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES LA English DT Article DE Hydroxytyrosol; Tyrosol; Bilayer depth; Partitioning ID SUPPORTED LIPID-BILAYERS; PHASE-TRANSITION TEMPERATURE; QUARTZ-CRYSTAL MICROBALANCE; VIRGIN OLIVE OIL; PHOSPHATIDYLCHOLINE VESICLES; CUPHEA OIL; IN-SITU; QCM-D; MEMBRANE; ADSORPTION AB The phenols hydroxytyrosol and tyrosol made abundantly available through olive oil processing were enzymatically transesterified into effective lipophilic antioxidants with cuphea oil. The hydroxytyrosyl and tyrosyl esters made from cuphea oil were assessed for their ability to partition into, locate within and effect the bilayer behavior of 1,2-dioloeoylphosphatidylcholine liposomes and compared to their counterparts made from decanoic acid. Partitioning into liposomes was on the same scale for both hydroxytyrosyl derivatives and both tyrosyl derivatives. All were found to locate nearly at the same depth within the bilayer. Each was found to affect bilayer behavior in a distinct manner. Published by Elsevier B.V. C1 [Evans, Kervin O.; Laszlo, Joseph A.; Compton, David L.] USDA ARS, Natl Ctr Agr Utilizat Res Ctr, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. RP Evans, KO (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res Ctr, Renewable Prod Technol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Kervin.Evans@ars.usda.gov NR 42 TC 2 Z9 2 U1 2 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0005-2736 EI 0006-3002 J9 BBA-BIOMEMBRANES JI Biochim. Biophys. Acta-Biomembr. PD MAY PY 2015 VL 1848 IS 5 BP 1175 EP 1182 DI 10.1016/j.bbamem.2015.02.002 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA CE7SC UT WOS:000352041100012 PM 25687972 ER PT J AU Paris, TM Croxton, SD Stansly, PA Allan, SA AF Paris, Thomson M. Croxton, Scott D. Stansly, Phillip A. Allan, Sandra A. TI Temporal response and attraction of Diaphorina citri to visual stimuli SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE psyllids; colored light; LED; diurnal periodicity; activity; Liviidae; Hemiptera; Asian citrus pysllid ID KUWAYAMA HEMIPTERA PSYLLIDAE; ADULT ASIAN CITRUS; HOST PLANTS; XYLEM FLUID; TRIALEURODES-VAPORARIORUM; PHYTOPHAGOUS INSECTS; SPECTRAL SENSITIVITY; STICKY TRAPS; LIGHT; MANAGEMENT AB As the vector of the global disease of citrus greening or huanglongbing, Asian citrus pysllids, Diaphorina citri Kuwayama (Hemiptera: Liviidae), are the greatest threat to the worldwide citrus industry. Critical to management of D.citri and huanglongbing is optimization of surveillance methodologies. Although phytophagous insects may find host plants by multimodal cues, some appear to primarily use visual cues. In this study, we examined the behavior of Asian citrus psyllids toward light from light-emitting diodes (LEDs) in the insect visible spectrum. The periodicity of attraction of psyllids to visual cues was evaluated in the field (yellow sticky traps) and laboratory (multi-colored LEDs) with a strong peak of activity during the afternoon in both the field and the laboratory (both 14:00 to 18:00hours). In laboratory evaluations of psyllids to differently colored LEDs, strongest attraction was to LEDs emitting ultraviolet (390nm), green (525nm), and yellow (590nm) light. Male and female psyllids did not differ significantly in their responses to visual cues. These findings provide the basis for formulating better traps that reflect UV and yellow light and potentially incorporate UV LEDs for monitoring psyllids and a better understanding of Asian citrus psyllid visual behavior. C1 [Paris, Thomson M.; Croxton, Scott D.; Stansly, Phillip A.] Southwest Citrus Res & Extens Ctr, Dept Entomol & Nematol, Immokalee, FL 34142 USA. [Paris, Thomson M.; Allan, Sandra A.] USDA ARS, Insect Behav & Biocontrol Res Unit, Gainesville, FL 32608 USA. RP Allan, SA (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32508 USA. EM sandy.allan@ars.usda.gov FU Citrus Research and Development Foundation FX We thank Eric Paulsen for assistance when building the bioassay arena, Larry Pitts for invaluable help with development of the variable resistance box, and Heidi Burnside and Jane Sharp for psyllid colony maintenance. Funding was provided in part by the Citrus Research and Development Foundation. NR 68 TC 6 Z9 6 U1 4 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAY PY 2015 VL 155 IS 2 BP 137 EP 147 DI 10.1111/eea.12294 PG 11 WC Entomology SC Entomology GA CF5PJ UT WOS:000352609000006 ER PT J AU Zhao, F Keane, R Zhu, ZL Huang, CQ AF Zhao, Feng Keane, Robert Zhu, Zhiliang Huang, Chengquan TI Comparing historical and current wildfire regimes in the Northern Rocky Mountains using a landscape succession model SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire regimes; FRCC; LANDSUMv4 model; MTBS; LANDFIRE; Northern Rocky Mountains ID NORMALIZED BURN RATIO; CURRENT FIRE REGIMES; WILDLAND FIRE; UNITED-STATES; TIME-SERIES; VEGETATION; MANAGEMENT; SEVERITY; SIMULATION; LANDSAT AB Wildland fire is a key ecosystem process that shapes the landscape of Western United States. Changes in fire regimes can therefore have profound impact on ecosystem functions and services, including carbon cycling, habitat conditions, and biodiversity. This study examined fire regime changes in the Northern Rocky Mountain region using a landscape succession model (LANDSUMv4). The objective is to report a new method to develop fire regime condition class (FRCC) maps by comparing historical reference conditions of fire regimes simulated using historical fire record and the LANDSUMv4 model - with current fire regimes developed using 27 years remote sensing fire product and LANDSUMv4. Results of this study indicate that most forest ecosystems in the study region experienced less frequent but more severe fires during the contemporary time period compared to the historical conditions. Fire regimes have changed the most for montane forests which historically were dominated by frequent and nonlethal fires. Forest ecosystems characterized by infrequent stand replacement fires also experienced moderate departure from historical fire conditions. FRCC assessment method proposed in this study can be applied to other areas of U.S. The findings of this study will help reveal contemporary fire dynamics in this region and serve for future fire and fuel studies and other forest management applications. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhao, Feng; Huang, Chengquan] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Keane, Robert] US Forest Serv, USDA, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59807 USA. [Zhu, Zhiliang] US Geol Survey, Reston, VA 20192 USA. RP Zhao, F (reprint author), Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. EM fengzhao@umd.edu OI Huang, Chengquan/0000-0003-0055-9798 FU USDA Forest Service Remote Sensing Applications Center; USGS Earth Resources Observation Systems Data Center; Wild land Fire Leadership Council; Department of Interior, US Geological Survey FX We thank the two anonymous reviewers for their helpful reviews and comments. Burned area boundary and burn severity data was provided by the Monitoring Trends in Burn Severity Project supported by USDA Forest Service Remote Sensing Applications Center and the USGS Earth Resources Observation Systems Data Center. Historical fire probability data and historical LANDUSMv4 model parameterization were provided by the LANDFIRE Project sponsored by multiple partners under the Wild land Fire Leadership Council. Funding support for this research was provided by the Department of Interior, US Geological Survey. We thank Greg Dillon, Brendan Ward, Robin Innes and Don Long for providing data assistance and sharing their many helpful comments. NR 59 TC 2 Z9 2 U1 2 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2015 VL 343 BP 9 EP 21 DI 10.1016/j.foreco.2015.01.020 PG 13 WC Forestry SC Forestry GA CF0PF UT WOS:000352246000002 ER PT J AU Lamba, J Thompson, AM Karthikeyan, KG Fitzpatrick, FA AF Lamba, Jasmeet Thompson, A. M. Karthikeyan, K. G. Fitzpatrick, Faith A. TI Sources of fine sediment stored in agricultural lowland streams, Midwest, USA SO GEOMORPHOLOGY LA English DT Article DE Sediment sources; Stream bed sediment; Sediment tracers; Land use; Stream bank erosion; Sediment fingerprinting ID EFFECTS ASSESSMENT PROJECT; SUSPENDED-SEDIMENT; RIVER-BASINS; CATCHMENT; UK; PHOSPHORUS; MANAGEMENT; VALLEY; ALLUVIATION; WATERSHEDS AB Agricultural activities can accelerate the offsite transport of productive soil from fields leading to stream water quality degradation. Identification of the nature and relative contribution of different sources to fine-grained sediment (e.g., silts, clays) in streams is important to effectively focus agricultural best management practices in watersheds. Sediment fingerprinting techniques through the use of geochemical tracers are commonly used to differentiate relative contribution from various sources. Research was conducted in lowland streams in the Pleasant Valley watershed in South Central Wisconsin (USA) to identify provenance of fine-grained sediment deposits and evaluate the impact of land use on relative contributions from the following potential sources: cropland, pasture, woodland, and eroding stream banks. Results show that both agriculture (croplands and pastures) and eroding stream banks are primary sources to fine sediment deposits on the stream bed with contributions ranging from 19 to 100% and 0 to 81%, respectively. The increase in area under agricultural land use within a subwatershed results in greater contribution from agriculture (R-2 = 0.846, p = 0.0034). Relative contributions from eroding stream banks increased with increasing area under grasslands and woodlands within a subwatershed (R-2 = 0.814, p = 0.0055). Subwatersheds with greater mass of fine sediment deposited on the stream bed per unit area should be prioritized for best management practices. The conservation practices should be targeted to stream banks or croplands depending on the dominant source of fine sediment within a subwatershed. Site specific changes in relative contributions from different sources to fine-grained sediment in this watershed highlights the complexities involved in sediment transport dynamics. The nested sampling sites helped determine that sediment dynamics at the subwatershed scale need to be considered for application of targeted conservation techniques. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lamba, Jasmeet; Thompson, A. M.; Karthikeyan, K. G.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Lamba, Jasmeet] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Fitzpatrick, Faith A.] US Geol Survey, Middleton, WI 53562 USA. RP Lamba, J (reprint author), Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. EM jul59@psu.edu RI LAMBA, JASMEET/B-3878-2015 FU USDA NIFA award [2009-51130-06049]; Nature Conservancy; Wisconsin Department of Natural Resources FX Funding for this project was provided by the USDA NIFA award #2009-51130-06049, The Nature Conservancy, and Wisconsin Department of Natural Resources. We thank John Panuska (University of Wisconsin-Madison, Biological Systems Engineering) and Laura Good (University of Wisconsin-Madison, Soils Department) for their technical guidance and assistance with collecting samples, Zach Zopp (University of Wisconsin-Madison, Biological Systems Engineering) for his assistance with sample collection, Allen Gellis (U.S. Geological Survey) for his valuable comments and suggestions, and staff at DCLCD, Dane County, Wisconsin, who helped with landowner communication and sediment sampling. Hydrologic technicians and student interns from the U.S. Geological Survey assisted with collection of upland soil, stream bank, and stream-bed fine sediment. Lastly, we thank all the landowners in this watershed for allowing access to sampled fields and streams. NR 58 TC 4 Z9 4 U1 8 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X EI 1872-695X J9 GEOMORPHOLOGY JI Geomorphology PD MAY 1 PY 2015 VL 236 BP 44 EP 53 DI 10.1016/j.geomorph.2015.02.001 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CF6OW UT WOS:000352677000004 ER PT J AU Saatchi, S Mascaro, J Xu, L Keller, M Yang, Y Duffy, P Espirito-Santo, FDB Baccini, A Chambers, J Schimel, D AF Saatchi, Sassan Mascaro, Joseph Xu, Liang Keller, Michael Yang, Yan Duffy, Paul Espirito-Santo, Fernando D. B. Baccini, Alessandro Chambers, Jeffery Schimel, David TI Seeing the forest beyond the trees SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Letter DE Allometry; biomass; lidar; remote sensing; spatial modelling; tree height; tropical forests; wood density ID ABOVEGROUND LIVE BIOMASS; WOOD SPECIFIC-GRAVITY; TROPICAL FOREST; CARBON-DENSITY; PATTERNS; AMAZONIA; STOCKS; PANAMA; BASIN; LIDAR AB In a recent paper (Mitchard etal. 2014, Global Ecology and Biogeography, 23, 935-946) a new map of forest biomass based on a geostatistical model of field data for the Amazon (and surrounding forests) was presented and contrasted with two earlier maps based on remote-sensing data Saatchi etal. (2011; RS1) and Baccini etal. (2012; RS2). Mitchard etal. concluded that both the earlier remote-sensing based maps were incorrect because they did not conform to Mitchard etal. interpretation of the field-based results. In making their case, however, they misrepresented the fundamental nature of primary field and remote-sensing data and committed critical errors in their assumptions about the accuracy of research plots, the interpolation methodology and the statistical analysis. By ignoring the large uncertainty associated with ground estimates of biomass and the significant under-sampling and spatial bias of research plots, Mitchard etal. reported erroneous trends and artificial patterns of biomass over Amazonia. Because of these misrepresentations and methodological flaws, we find their critique of the satellite-derived maps to be invalid. C1 [Saatchi, Sassan; Espirito-Santo, Fernando D. B.; Schimel, David] CALTECH, NASA Jet Prop Lab, Pasadena, CA 91109 USA. [Saatchi, Sassan; Xu, Liang; Yang, Yan] Univ Calif Los Angeles, Inst Environm & Sustainabil, Los Angeles, CA 90095 USA. [Mascaro, Joseph] Amer Assoc Advancement Sci, Washington, DC 20001 USA. [Keller, Michael] US Forest Serv, USDA, Int Inst Trop Forestry, San Juan, PR USA. [Duffy, Paul] Neptune & Co Inc, Lakewood, CO 80215 USA. [Baccini, Alessandro] Woods Hole Res Ctr, Falmouth, MA 02540 USA. [Chambers, Jeffery] Univ Calif Berkeley, Dept Geog, Berkeley, CA 94720 USA. RP Saatchi, S (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. EM saatchi@jpl.nasa.gov RI Keller, Michael/A-8976-2012; Chambers, Jeffrey/J-9021-2014; Espirito-Santo, Fernando/O-4371-2014 OI Keller, Michael/0000-0002-0253-3359; Chambers, Jeffrey/0000-0003-3983-7847; Espirito-Santo, Fernando/0000-0001-7497-3639 NR 39 TC 12 Z9 12 U1 4 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD MAY PY 2015 VL 24 IS 5 BP 606 EP 610 DI 10.1111/geb.12256 PG 5 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CF4OY UT WOS:000352530900010 ER PT J AU Bauer, PJ Stone, KC Foulk, JA Dodd, RB AF Bauer, Philip J. Stone, Kenneth C. Foulk, Jonn A. Dodd, Roy B. TI Irrigation and cultivar effect on flax fiber and seed yield in the Southeast USA SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Flax; Irrigation; Fiber ID WATER AB Flax (Linum usitatissimum L.) is a potential winter crop for the Southeast USA that can be grown for both seed and fiber. The objective of this research was to evaluate the effect of irrigation on flax straw, fiber, and seed yield of fiber-type and seed-type cultivars at different flax growth stages. The study was conducted during the winter growing seasons of 2010/2011, 2011/2012, and 2012/2013 near Florence, SC. Four fiber-type cultivars and one seed-type cultivar were grown with and without irrigation for two years. The four fiber-types were evaluated for straw and fiber yield in the third year. Soil water was monitored to trigger irrigations. Irrigation was applied before all four harvests in 2010/2011, before only the last harvest in 2011/2012, and was not applied in 201212013. Straw harvests were made at the onset of flowering, 10 days past the onset of flowering, 20 days past the onset of flowering, and when seeds were mature. Seed harvests were made at the end of the 2011/2012 and 2012/2013 growing seasons. In 2010/2011, plots had to be replanted in February so crop development was delayed. Irrigation increased straw yield at the last three harvests in that year. In the other two years, when planting occurred at normal times in the fall, irrigation did not influence straw or fiber yield. Irrigation had no significant effect on seed yield. The fiber-type cultivars did not differ for straw or fiber yield. At the onset of flowering harvest, the seed-type cultivar had similar fiber content to the fiber-type cultivars. The fiber-type cultivars had higher fiber content in later harvests. The results support previous research in that fiber-type cultivars appear viable for production as fiber winter crops in the region. The results also suggest that high straw yielding seed-type cultivars could be used, especially in systems with early straw harvests. Published by Elsevier B.V. C1 [Bauer, Philip J.; Stone, Kenneth C.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Foulk, Jonn A.] FX Fibers LLC, Clemson, SC USA. [Dodd, Roy B.] Clemson Univ, Clemson, SC USA. RP Bauer, PJ (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM phil.bauer@ars.usda.gov NR 12 TC 0 Z9 0 U1 5 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2015 VL 67 BP 7 EP 10 DI 10.1016/j.indcrop.2014.12.053 PG 4 WC Agricultural Engineering; Agronomy SC Agriculture GA CE7RN UT WOS:000352039600002 ER PT J AU Klasson, KT Uchimiya, M Lima, IM AF Klasson, K. Thomas Uchimiya, Minori Lima, Isabel M. TI Characterization of narrow micropores in almond shell biochars by nitrogen, carbon dioxide, and hydrogen adsorption SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Pore size distribution; Nanopores; Washing; Normalized adsorption isotherm; Characteristic curve ID ACTIVATED CARBONS; SURFACE-AREA; CO2; CHARCOAL; CONTAMINANTS; TEMPERATURE; ADSORBENTS; PRECURSORS; STONES; MANURE AB Characterization of biochars usually includes surface area and pore volume determinations by nitrogen adsorption. In this study, we confirmed that there is a substantial pore volume in biochars created via slow pyrolysis from low- and high-ash almond shells that cannot be characterized in this fashion due to the narrow pore sizes. Hydrogen and carbon dioxide were used, in addition to nitrogen, to characterize these narrow micropores. All isotherms, when appropriately normalized, fell on the same characteristic curve which aided in the characterization and understanding of the pore structure. Based on the characteristic adsorption isotherm, the average pore size diameter was estimated to be 0.4-0.8 nm. When washed, the biochars' pore volume increased but the narrow micropore structure remained. While the biochars were alkaline, the pH of the wash solution had no or little impact on the adsorption of CO2. Overall, the results suggest that N-2 isotherms should be complemented with CO2 isotherms for proper characterization of biochars. Alignment of such normalized isotherms to characteristic curves can assist in generating a more complete understanding of the pore structure over the entire region of pore diameters. Published by Elsevier B.V. C1 [Klasson, K. Thomas; Uchimiya, Minori; Lima, Isabel M.] USDA ARS, New Orleans, LA 70124 USA. RP Klasson, KT (reprint author), USDA ARS, New Orleans, LA 70124 USA. EM thomas.klasson@ars.usda.gov NR 51 TC 3 Z9 5 U1 3 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2015 VL 67 BP 33 EP 40 DI 10.1016/j.indcrop.2015.01.010 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA CE7RN UT WOS:000352039600006 ER PT J AU Ramchandran, D Johnston, DB Tumbleson, ME Rausch, KD Singh, V AF Ramchandran, Divya Johnston, David B. Tumbleson, M. E. Rausch, Kent D. Singh, Vijay TI Seasonal variability in ethanol concentrations from a dry grind fermentation operation associated with incoming corn variability SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Ethanol; Fermentation; Dry grind process; Grain quality variation ID PROTEASE; ENDOSPERM AB Corn from an ethanol plant (commodity corn) and an identity preserved corn hybrid from a seed company (control corn stored at 4 degrees C) were used to study the effects of incoming corn on dry grind ethanol concentrations. Ethanol concentrations were determined every 2 weeks for 1 year using conventional dry grind procedure. Variations in ethanol concentrations were significant and variability patterns for commodity and control corn followed the same trend. Highest ethanol concentrations were seen in the month of January. Variation with control corn suggested that storage time is a significant factor affecting ethanol concentrations. Effects of different enzyme treatments on mean ethanol concentration over a year were evaluated. Two liquefaction enzymes (optimum pH - 5.8 and 5.1, respectively), two saccharification enzymes (optimum pH - 5.0) and one protease were used in five enzyme treatments (I-V). Final ethanol concentration with enzyme treatment V was (17.5 +/- 0.486)%v/v. This was 0.6% higher than enzyme treatment I resulting in an additional ethanol production of 600,000 gallons/year in a 100 million gallon/year ethanol plant. Using more effective enzymes increases overall dry grind ethanol production and ethanol plant profitability. (C) 2015 Elsevier B.V. All rights reserved. C1 [Ramchandran, Divya; Tumbleson, M. E.; Rausch, Kent D.; Singh, Vijay] Univ Illinois, Agr & Biol Engn, Urbana, IL 61801 USA. [Johnston, David B.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Singh, V (reprint author), Univ Illinois, Agr & Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu NR 16 TC 3 Z9 3 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2015 VL 67 BP 155 EP 160 DI 10.1016/j.indcrop.2015.01.029 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CE7RN UT WOS:000352039600022 ER PT J AU Cermak, SC Durham, AL Isbell, TA Evangelista, RL Murray, RE AF Cermak, Steven C. Durham, Amber L. Isbell, Terry A. Evangelista, Roque L. Murray, Rex E. TI Synthesis and physical properties of pennycress estolides and esters SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biodegradable oils; Estolide; Pennycress; Pour point; Viscosity ID ACID-CATALYZED CONDENSATION; OLEIC-ACID; 2-ETHYLHEXYL ESTERS; OIL AB A new series of pennycress (Thlaspi arvense L.) based free-acid estolides was synthesized by an acid-catalyzed condensation reaction, followed by an esterification reaction to produce the 2-ethylhexyl (2-EH) esters of the initial estolides. The physical properties of the estolides are highly affected by the length and unsaturation level of the capping fatty acid, the base fatty acid unit, and estolide linkage position. Both the free-acid estolides and the estolide 2-EH esters produced proved to have marked viscosity increases over previously synthesized estolides. Kinematic viscosities of the free-acid estolides were higher than the corresponding estolide 2-EH esters, ranging from 494.4 cSt to 870.5 cSt at 40 degrees C and 53.6 to 75.3 cSt at 100 degrees C with viscosity indices (VI) from 134 to 163. Viscosities of the estolide esters ranged from 116.3 to 245.75 cSt at 40 degrees C and 18.2 to 33.6 cSt at 100 degrees C with viscosity indices (VI) from 169 to 183. The highest viscosity values belonged to the pennycress estolides made with pennycress fatty acids in the reaction acting as the base material, as well as the capping material. The oleic capped pennycress estolide 2-EH esters had the best low-temperature properties with a pour point (PP) of-33 degrees C and cloud point (CP) of-31 degrees C. With the exception of palmitic acid, there was very little variation in the low-temperature properties of the remaining saturate capped estolide 2-EH esters, PP ranging from 18 to 21 degrees C. Pennycress is currently being developed for green diesel applications; the development of other industrial applications is a necessity for the success of an alternative crop. The combination of high viscosity and modest cold temperature properties of these pennycress estolides could fill a specialty niche as a high viscosity industrial lubricant. Published by Elsevier B.V. C1 [Cermak, Steven C.; Durham, Amber L.; Isbell, Terry A.; Evangelista, Roque L.; Murray, Rex E.] USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, Peoria, IL 61604 USA. RP Cermak, SC (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM steven.cermak@ars.usda.gov NR 23 TC 3 Z9 3 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2015 VL 67 BP 179 EP 184 DI 10.1016/j.indcrop.2015.01.050 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA CE7RN UT WOS:000352039600025 ER PT J AU Luethy, PM Huynh, S Parker, CT Hendrixson, DR AF Luethy, Paul M. Huynh, Steven Parker, Craig T. Hendrixson, David R. TI Analysis of the Activity and Regulon of the Two-Component Regulatory System Composed by Cjj81176_1484 and Cjj81176_1483 of Campylobacter jejuni SO JOURNAL OF BACTERIOLOGY LA English DT Article ID SIGNAL-TRANSDUCTION; COLONIZATION; IDENTIFICATION; PROTEIN; INFECTION; PHOSPHATE; GROWTH; GENES; PEB3; PHOSPHORYLATION AB Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper expression of specific genes. We analyzed the potential of C. jejuni Cjj81176_1484 (Cjj1484) and Cjj81176_1483 (Cjj1483) to encode proteins of a cognate TCS that influences expression of genes possibly important for C. jejuni growth and colonization. Transcriptome analysis revealed that the regulons of the Cjj81176_1484 (Cjj1484) histidine kinase and the Cjj81176_1483 (Cjj1483) response regulator contain many common genes, suggesting that these proteins likely form a cognate TCS. We found that this TCS generally functions to repress expression of specific proteins with roles in metabolism, iron/heme acquisition, and respiration. Furthermore, the TCS repressed expression of Cjj81176_0438 and Cjj81176_0439, which had previously been found to encode a gluconate dehydrogenase complex required for commensal colonization of the chick intestinal tract. However, the TCS and other specific genes whose expression is repressed by the TCS were not required for colonization of chicks. We observed that the Cjj1483 response regulator binds target promoters in both unphosphorylated and phosphorylated forms and influences expression of some specific genes independently of the Cjj1484 histidine kinase. This work further expands the signaling mechanisms of C. jejuni and provides additional insights regarding the complex and multifactorial regulation of many genes involved in basic metabolism, respiration, and nutrient acquisition that the bacterium requires for optimal growth in different environments. IMPORTANCE Bacterial two-component regulatory systems (TCSs) link environmental cues to expression of specific genes that enable optimal bacterial growth or colonization of hosts. We found that the Campylobacter jejuni Cjj1484 histidine kinase and Cjj1483 response regulator function as a cognate TCS to largely repress expression of target genes encoding a gluconate dehydrogenase complex required for commensal colonization of the chick intestinal tract, as well as other genes encoding proteins for heme or iron acquisition, metabolism, and respiration. We also discovered different modes by which Cjj1483 may mediate repression with and without Cjj1484. This work provides insight into the signal transduction mechanisms of a leading cause of bacterial diarrheal disease and emphasizes the multifactorial and complex regulation of specific biological processes in C. jejuni. C1 [Luethy, Paul M.; Hendrixson, David R.] Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA. [Huynh, Steven; Parker, Craig T.] USDA ARS, Produce Safety & Microbiol, Albany, CA USA. RP Hendrixson, DR (reprint author), Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA. EM david.hendrixson@utsouthwestern.edu FU NIH [R01AI065539, R21AI103643, T32 AI007520]; USDA, Agricultural Research Service (CRIS project) [5325-42000-230-047] FX This work was supported by NIH grants R01AI065539 (D.R.H.) and R21AI103643 (D.R.H.). P.M.L. was supported by NIH training grant T32 AI007520. This work was also funded by the USDA, Agricultural Research Service (CRIS project 5325-42000-230-047). NR 55 TC 1 Z9 1 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 EI 1098-5530 J9 J BACTERIOL JI J. Bacteriol. PD MAY PY 2015 VL 197 IS 9 BP 1592 EP 1605 DI 10.1128/JB.02564-14 PG 14 WC Microbiology SC Microbiology GA CF1HU UT WOS:000352296500006 PM 25691530 ER PT J AU Shoemaker, CA LaFrentz, BR AF Shoemaker, C. A. LaFrentz, B. R. TI Lack of association between Flavobacterium columnare genomovar and virulence in hybrid tilapia Oreochromis niloticus (L.) x Oreochromis aureus (Steindachner) SO JOURNAL OF FISH DISEASES LA English DT Article DE columnaris disease; Flavobacterium columnare; genomovar; tilapia; virulence ID CATFISH ICTALURUS-PUNCTATUS; TROUT ONCORHYNCHUS-MYKISS; CHANNEL CATFISH; BINDING LECTIN; FISH; CHALLENGE; DISEASE; MUCUS AB Columnaris disease can be problematic in tilapia (Oreochromis spp.) production. An understanding of the pathogenesis and virulence of Flavobacterium columnare is needed to develop prevention strategies. The objective of this study was to determine the virulence of genetically defined isolates of F.columnare in sex-reversed hybrid tilapia, Oreochromis niloticus (L.)xO.aureus (Steindachner). A series of immersion challenge trials were performed using isolates of the five established genomovars of F.columnare: I, II, II-B, III and I/II. The mean per cent mortality of fish challenged with genomovar I, II and III isolates ranged from 0 to 100, 3.3-78 and 3.3-75%, respectively. The mean per cent mortality of fish challenged with genomovar II-B ranged from 35 to 96.7%, and the only genomovar I/II isolate tested caused no mortality. Contrary to previous work in other fish species, there did not appear to be an association between F.columnare genomovar and virulence in tilapia. The challenge model used resulted in acute mortality. An alternative challenge model was tested by cohabitating healthy fish with dead fish infected with F.columnare. This method resulted in rapid appearance of clinical signs and mortality, suggesting the potential for F.columnare to increase in virulence upon growth on/in a fish host. C1 [Shoemaker, C. A.; LaFrentz, B. R.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Shoemaker, CA (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM craig.shoemaker@ars.usda.gov FU USDA-ARS CRIS Project [6420-32000-024-00D] FX The authors acknowledge the technical contributions of Mrs. Paige Mumma and Dr. Julio Garcia of USDA-ARS (Aquatic Animal Health Research Unit). The authors are grateful to Drs. John Drennan (Merck Animal Health, Elkhorn, NE) and Jason Evenhuis (National Center for Cool and Cold Water Aquaculture (NCCWA, Kearneysville, WV) for critical review of the manuscript prior to submission. This research was supported by USDA-ARS CRIS Project No. 6420-32000-024-00D (Integrated Aquatic Animal Health Strategies). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 26 TC 6 Z9 6 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD MAY PY 2015 VL 38 IS 5 BP 491 EP 498 DI 10.1111/jfd.12262 PG 8 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA CF5FO UT WOS:000352582600007 PM 24909885 ER PT J AU Marzano, SYL Hobbs, HA Nelson, BD Hartman, GL Eastburn, DM McCoppin, NK Domier, LL AF Marzano, Shin-Yi Lee Hobbs, Houston A. Nelson, Berlin D. Hartman, Glen L. Eastburn, Darin M. McCoppin, Nancy K. Domier, Leslie L. TI Transfection of Sclerotinia sclerotiorum with In Vitro Transcripts of a Naturally Occurring Interspecific Recombinant of Sclerotinia sclerotiorum Hypovirus 2 Significantly Reduces Virulence of the Fungus SO JOURNAL OF VIROLOGY LA English DT Article ID PLANT-PATHOGENIC FUNGUS; CHESTNUT BLIGHT FUNGUS; DOUBLE-STRANDED-RNA; MULTIPLE SEQUENCE ALIGNMENT; INFECTIOUS CDNA-CLONE; TURNIP-MOSAIC-VIRUS; CRYPHONECTRIA-PARASITICA; MOLECULAR CHARACTERIZATION; MYCOVIRUS TRANSMISSION; CONFERS HYPOVIRULENCE AB A recombinant strain of Sclerotinia sclerotiorum hypovirus 2 (SsHV2) was identified from a North American Sclerotinia sclerotiorum isolate (328) from lettuce (Lactuca sativa L.) by high-throughput sequencing of total RNA. The 5' -and 3' -terminal regions of the genome were determined by rapid amplification of cDNA ends. The assembled nucleotide sequence was up to 92% identical to two recently reported SsHV2 strains but contained a deletion near its 5' terminus of more than 1.2 kb relative to the other SsHV2 strains and an insertion of 524 nucleotides (nt) that was distantly related to Valsa ceratosperma hypovirus 1. This suggests that the new isolate is a heterologous recombinant of SsHV2 with a yet-uncharacterized hypovirus. We named the new strain Sclerotinia sclerotiorum hypovirus 2 Lactuca (SsHV2L) and deposited the sequence in GenBank with accession number KF898354. Sclerotinia sclerotiorum isolate 328 was coinfected with a strain of Sclerotinia sclerotiorum endornavirus 1 and was debilitated compared to cultures of the same isolate that had been cured of virus infection by cycloheximide treatment and hyphal tipping. To determine whether SsHV2L alone could induce hypovirulence in S. sclerotiorum, a full-length cDNA of the 14,538-nt viral genome was cloned. Transcripts corresponding to the viral RNA were synthesized in vitro and transfected into a virus-free isolate of S. sclerotiorum, DK3. Isolate DK3 transfected with SsHV2L was hypovirulent on soybean and lettuce and exhibited delayed maturation of sclerotia relative to virus-free DK3, completing Koch's postulates for the association of hypovirulence with SsHV2L. IMPORTANCE A cosmopolitan fungus, Sclerotinia sclerotiorum infects more than 400 plant species and causes a plant disease known as white mold that produces significant yield losses in major crops annually. Mycoviruses have been used successfully to reduce losses caused by fungal plant pathogens, but definitive relationships between hypovirus infections and hypovirulence in S. sclerotiorum were lacking. By establishing a cause-and-effect relationship between Sclerotinia sclerotiorum hypovirus Lactuca (SsHV2L) infection and the reduction in host virulence, we showed direct evidence that hypoviruses have the potential to reduce the severity of white mold disease. In addition to intraspecific recombination, this study showed that recent interspecific recombination is an important factor shaping viral genomes. The construction of an infectious clone of SsHV2L allows future exploration of the interactions between SsHV2L and S. sclerotiorum, a widespread fungal pathogen of plants. C1 [Marzano, Shin-Yi Lee; Hobbs, Houston A.; Hartman, Glen L.; Eastburn, Darin M.; Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Nelson, Berlin D.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Hartman, Glen L.; McCoppin, Nancy K.; Domier, Leslie L.] ARS, USDA, Urbana, IL USA. RP Domier, LL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM leslie.domier@ars.usda.gov FU National Sclerotinia Initiative; United States Department of Agriculture/Agricultural Research Service FX This research was funded by the National Sclerotinia Initiative and the United States Department of Agriculture/Agricultural Research Service. NR 90 TC 10 Z9 10 U1 3 U2 32 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAY PY 2015 VL 89 IS 9 BP 5060 EP 5071 DI 10.1128/JVI.03199-14 PG 12 WC Virology SC Virology GA CF0FX UT WOS:000352219600032 PM 25694604 ER PT J AU Duke, SO AF Duke, Stephen O. TI Perspectives on transgenic, herbicide-resistant crops in the United States almost 20 years after introduction SO PEST MANAGEMENT SCIENCE LA English DT Article DE genetically modified crop; dicamba; glufosinate; glyphosate; herbicide-resistant crop; transgenic crop; weed management; 2; 4-D ID AMARANTH AMARANTHUS-PALMERI; GLYPHOSATE-RESISTANCE; COTTON; COMMUNITY; KNOWLEDGE; TILLAGE AB Herbicide-resistant crops have had a profound impact on weed management. Most of the impact has been by glyphosate-resistant maize, cotton, soybean and canola. Significant economic savings, yield increases and more efficacious and simplified weed management have resulted in widespread adoption of the technology. Initially, glyphosate-resistant crops enabled significantly reduced tillage and reduced the environmental impact of weed management. Continuous use of glyphosate with glyphosate-resistant crops over broad areas facilitated the evolution of glyphosate-resistant weeds, which have resulted in increases in the use of tillage and other herbicides with glyphosate, reducing some of the initial environmental benefits of glyphosate-resistant crops. Transgenic crops with resistance to auxinic herbicides, as well as to herbicides that inhibit acetolactate synthase, acetyl-CoA carboxylase and hydroxyphenylpyruvate dioxygenase, stacked with glyphosate and/or glufosinate resistance, will become available in the next few years. These technologies will provide additional weed management options for farmers, but will not have all of the positive effects (reduced cost, simplified weed management, lowered environmental impact and reduced tillage) that glyphosate-resistant crops had initially. In the more distant future, other herbicide-resistant crops (including non-transgenic ones), herbicides with new modes of action and technologies that are currently in their infancy (e.g. bioherbicides, sprayable herbicidal RNAi and/or robotic weeding) may affect the role of transgenic, herbicide-resistant crops in weed management. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 ARS, USDA, Nat Prod Utilizat Res, Thad Cochran Res Ctr, University, MS 38677 USA. RP Duke, SO (reprint author), ARS, USDA, Nat Prod Utilizat Res, Thad Cochran Res Ctr, University, MS 38677 USA. EM Stephen.Duke@ars.usda.gov NR 45 TC 13 Z9 13 U1 19 U2 112 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2015 VL 71 IS 5 BP 652 EP 657 DI 10.1002/ps.3863 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA CF5PO UT WOS:000352609500003 PM 25052888 ER PT J AU Boppre, M Colegate, SM AF Boppre, Michael Colegate, Steven M. TI Recognition of Pyrrolizidine Alkaloid Esters in the Invasive Aquatic Plant Gymnocoronis spilanthoides (Asteraceae) SO PHYTOCHEMICAL ANALYSIS LA English DT Article DE Amabiline; curassavine; cynaustraline; gymnocoronine; heleurine; intermedine; lycopsamine; N-oxides; PA-pharmacophagy; porous graphitic carbon; Senegal tea plant; spilanthine; supinine ID INCREASED COMPETITIVE ABILITY; N-OXIDES; DEHYDROPYRROLIZIDINE ALKALOIDS; HYPOTHESIS; DIVERSITY; EVOLUTION; SUPPORT; DEFENSE; GENUS; EICA AB IntroductionThe freshwater aquatic plant Gymnocoronis spilanthoides (Senegal tea plant, jazmin del banado, Falscher Wasserfreund) is an invasive plant in many countries. Behavioural observations of pyrrolizidine alkaloid-pharmacophagous butterflies suggested the presence of pyrrolizidine alkaloids in the plant. ObjectiveTo determine whether the attraction of the butterflies to the plant is an accurate indicator of pyrrolizidine alkaloids in G. spilanthoides. MethodsThe alkaloid fraction of a methanolic extract of G. spilanthoides was analysed using HPLC with electrospray ionisation MS and MS/MS. Two HPLC approaches were used, that is, a C-18 reversed-phase column with an acidic mobile phase, and a porous graphitic carbon column with a basic mobile phase. ResultsPyrrolizidine alkaloids were confirmed, with the free base forms more prevalent than the N-oxides. The major alkaloids detected were lycopsamine and intermedine. The porous graphitic carbon HPLC column, with basic mobile phase conditions, resulted in better resolution of more pyrrolizidine alkaloids including rinderine, the heliotridine-based epimer of intermedine. Based on the MS/MS and high-resolution MS data, gymnocoronine was tentatively identified as an unusual C9 retronecine ester with 2,3-dihydroxy-2-propenylbutanoic acid. Among several minor-abundance monoester pyrrolizidines recognised, spilanthine was tentatively identified as an ester of isoretronecanol with the unusual 2-acetoxymethylbutanoic acid. ConclusionsThe butterflies proved to be reliable indicators for the presence of pro-toxic 1,2-dehydropyrrolizidine alkaloids in G. spilanthoides, the first aquatic plant shown to produce these alkaloids. The presence of the anti-herbivory alkaloids may contribute to the plant's invasive capabilities and would certainly be a consideration in any risk assessment of deliberate utilisation of the plant. The prolific growth of the plant and the structural diversity of its pyrrolizidine alkaloids may make it ideal for investigating biosynthetic pathways or for large-scale production of specific alkaloids. Copyright (c) 2015 John Wiley & Sons, Ltd. The presence of potentially toxic dehydropyrrolizidine alkaloid esters in the neotropical, freshwater aquatic plant Gymnocoronis spilanthoides was indicated by attraction of pyrrolizidine alkaloid-pharmacophagous butterflies to the plant. The HPLC-ESI(+)MS and MS/MS analysis of the alkaloidal fraction from a methanolic extract of the plant confirmed the presence of the dehydropyrrolizidine alkaloids lycopsamine, intermedine and rinderine along with several other more minor pyrrolizidine and dehydropyrrolizidine alkaloids. Tentative structures for two new pyrrolizidine alkaloids are proposed. C1 [Boppre, Michael] Univ Freiburg, Forstzool & Entomol, D-79085 Freiburg, Germany. [Colegate, Steven M.] ARS, USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Colegate, SM (reprint author), ARS, USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. EM steven.colegate@ars.usda.gov RI Boppre, Michael/P-4346-2016 NR 38 TC 4 Z9 4 U1 2 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0958-0344 EI 1099-1565 J9 PHYTOCHEM ANALYSIS JI Phytochem. Anal. PD MAY-JUN PY 2015 VL 26 IS 3 BP 215 EP 225 DI 10.1002/pca.2555 PG 11 WC Biochemical Research Methods; Plant Sciences; Chemistry, Analytical SC Biochemistry & Molecular Biology; Plant Sciences; Chemistry GA CF5GM UT WOS:000352585400005 PM 25645745 ER PT J AU Kamo, K Lakshman, D Bauchan, G Rajasekaran, K Cary, J Jaynes, J AF Kamo, Kathryn Lakshman, Dilip Bauchan, Gary Rajasekaran, Kanniah Cary, Jeffrey Jaynes, Jesse TI Expression of a synthetic antimicrobial peptide, D4E1, in Gladiolus plants for resistance to Fusarium oxysporum f. sp gladioli SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Flower bulb crops; Antifungal; Ornamentals; Transformation; Confocal laser scanning microscopy ID DISEASE RESISTANCE; IN-VITRO; GENE; TOMATO; FUNGI; ARABIDOPSIS; MECHANISMS; PROTEIN; TOBACCO; GROWTH AB The main pathogen of Gladiolus plants is Fusarium oxysporum, a soilborne fungus that infects roots and corms resulting in death of the plant. Purified D4E1, a synthetic antimicrobial peptide, was previously reported (De Lucca et al. 1998) to inhibit F. oxysporum spores from forming mycelial colonies in vitro at a concentration of 3 lM making it a candidate gene for genetic engineering of Gladiolus for resistance to F. oxysporum. Gladiolus cv. Peter Pears plants were transformed by particle bombardment with plasmid DNA containing a 90 bp D4E1 gene that was under the control of the duplicated CaMV 35S promoter. Five of the 14 independently transformed plant lines were evaluated for resistance to F. oxysporum. Transgenic plants were tested in vitro for resistance to F. oxysporum, and several lines appeared to be more resistant than the control plants that lacked D4E1. Cell extracts of transgenic Gladiolus lines 6(1) and 7(1) inhibited germinated spores of F. oxysporum f. sp. gladioli from forming mycelial colonies by 34 and 38 %, respectively, in vitro. F. oxysporum f. sp. gladioli was transformed with the ECFP (cyan) gene allowing us to follow the growth of F. oxysporum during infection of D4E1-transformed and nontransformed roots. Fluorescence observations using confocal laser scanning microscopy showed that 3-10 days after infection, F. oxysporum covered the surface of the root and formed pseudo-appressoria, but hyphae were never observed to penetrate cells of the root. Ten days after infection with F. oxysporum, non-transformed roots had completely disintegrated whereas transgenic roots of line 7(1) were just beginning to lose their cellular integrity. Cell extracts from the five transgenic lines showed either an inhibition of F. oxysporum mycelial colony formation or less fungal hyphae were observed to infect their roots as compared to non-transformed Gladiolus plants. C1 [Kamo, Kathryn; Lakshman, Dilip] Natl Arboretum, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD 20002 USA. [Bauchan, Gary] USDA, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. [Rajasekaran, Kanniah; Cary, Jeffrey] USDA, Food & Feed Safety Res Unit, So Reg Res Ctr, New Orleans, LA USA. [Jaynes, Jesse] Tuskegee Univ, Coll Agr Environm & Nat Sci, Tuskegee, AL 36088 USA. [Jaynes, Jesse] Tuskegee Univ, Coll Vet Med Nursing & Allied Hlth, Tuskegee, AL 36088 USA. RP Kamo, K (reprint author), Natl Arboretum, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD 20002 USA. EM Kathryn.Kamo@ars.usda.gov NR 33 TC 2 Z9 2 U1 2 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD MAY PY 2015 VL 121 IS 2 BP 459 EP 467 DI 10.1007/s11240-015-0716-4 PG 9 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CF6FQ UT WOS:000352652800018 ER PT J AU Regalado, JJ Carmona-Martin, E Castro, P Moreno, R Gil, J Encina, CL AF Regalado, J. J. Carmona-Martin, E. Castro, P. Moreno, R. Gil, J. Encina, C. L. TI Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Rhizome buds; Rooting; Molecular markers; Somaclonal variation; Asparagus brachyphyllus; Asparagus pseudoscaber ID SOMACLONAL VARIATION; MARKERS; RAPD; PROPAGATION; DIVERSITY; CULTURES; GROWTH; PLANTS AB An efficient micropropagation method for asparagus species was developed in this study. The method allows the fast cloning of the elite genotypes from different asparagus species and the interspecific hybrids obtained from these species. Rhizome bud explants were disinfected using 3 g l(-1) of benomyl and 20 g l(-1) of sodium hypochlorite. Then, they were cultured on Asparagus Rhizome Bud Medium 3 (ARBM-3) consisting in modified Mu-rashige and Skoog medium with salts with EDDHA-Fe (85.7 mg l(-1)) instead of EDTA-Fe and vitamins, supplemented with 0.3 mg l(-1) NAA, 0.1 mg l(-1) KIN, 2 mg l(-1) ancymidol and 6 % sucrose. Results showed that the method developed produced high disinfection rates (70-95 %). More than 70 % of the explants developed shoots and the rooting rate on ARBM-3 medium was 30-45 %. The rooting rate increased to 60-85 % when the unrooted shoots were subjected to an additional cycle of rooting, reaching 100 % after two cycles of rooting. The multiplication was achieved through mechanical division of rooted shoot clusters growing in ARBM-3. The acclimatization rate of the micropropagated plantlets was higher than 90 %. The micropopagated plantlets were screened for somaclonal variation using 12 expressed sequence tag derived simple sequence repeat markers. The results confirmed the character "true to type'' of the plantlets, indicating that the method developed in this study can be used to successfully micropropagate asparagus species. C1 [Regalado, J. J.; Carmona-Martin, E.; Encina, C. L.] CSIC UMA, Inst Hort Subtrop & Mediterranea La Mayora, Malaga 29750, Spain. [Castro, P.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. [Moreno, R.; Gil, J.] Univ Cordoba, ETSIAM, Dept Genet, E-14071 Cordoba, Spain. RP Regalado, JJ (reprint author), CSIC UMA, Inst Hort Subtrop & Mediterranea La Mayora, Malaga 29750, Spain. EM jjrg@eelm.csic.es; clencina@eelm.csic.es RI Moreno, Roberto/L-8999-2014; OI Moreno, Roberto/0000-0001-6114-2326; Gil, Juan/0000-0001-7938-442X; Regalado Gonzalez, Jose Javier/0000-0003-1664-2000 FU Junta de Andalucia [AGR3648] FX This project is funded by "Junta de Andalucia" (Project of Excellence AGR3648). NR 33 TC 3 Z9 3 U1 3 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD MAY PY 2015 VL 121 IS 2 BP 501 EP 510 DI 10.1007/s11240-015-0720-8 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CF6FQ UT WOS:000352652800022 ER PT J AU Patyk, KA Duncan, C Nol, P Sonne, C Laidre, K Obbard, M Wiig, O Aars, J Regehr, E Gustafson, LL Atwood, T AF Patyk, Kelly A. Duncan, Colleen Nol, Pauline Sonne, Christian Laidre, Kristin Obbard, Martyn Wiig, Oystein Aars, Jon Regehr, Eric Gustafson, Lori L. Atwood, Todd TI Establishing a definition of polar bear (Ursus maritimus) health: A guide to research and management activities SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Delphi; Polar bear; Survey; Ursus maritimus; Wildlife health ID ARCTIC MARINE MAMMALS; SOUTHERN BEAUFORT SEA; WILDLIFE HEALTH; CLIMATE-CHANGE; STRESS-RESPONSE; IMPACTS; SIZE; POPULATIONS; INSIGHTS; DISEASE AB The meaning of health for wildlife and perspectives on how to assess and measure health, are not well characterized. For wildlife at risk, such as some polar bear (Ursus maritimus) subpopulations, establishing comprehensive monitoring programs that include health status is an emerging need. Environmental changes, especially loss of sea ice habitat, have raised concern about polar bear health. Effective and consistent monitoring of polar bear health requires an unambiguous definition of health. We used the Delphi method of soliciting and interpreting expert knowledge to propose a working definition of polar bear health and to identify current concerns regarding health, challenges in measuring health, and important metrics for monitoring health. The expert opinion elicited through the exercise agreed that polar bear health is defined by characteristics and knowledge at the individual, population, and ecosystem level. The most important threats identified were in decreasing order: climate change, increased nutritional stress, chronic physiological stress, harvest management, increased exposure to contaminants, increased frequency of human interaction, diseases and parasites, and increased exposure to competitors. Fifteen metrics were identified to monitor polar bear health. Of these, indicators of body condition, disease and parasite exposure, contaminant exposure, and reproductive success were ranked as most important. We suggest that a cumulative effects approach to research and monitoring will improve the ability to assess the biological, ecological, and social determinants of polar bear health and provide measurable objectives for conservation goals and priorities and to evaluate progress. Published by Elsevier B.V. C1 [Patyk, Kelly A.; Gustafson, Lori L.] USDA, Ctr Epidemiol & Anim Hlth,Sci Technol & Anal Serv, Vet Serv, Anim & Plant Hlth Inspect Serv, Washington, DC USA. [Duncan, Colleen] Colorado State Univ, Colorado State Univ Vet Diagnost Lab, Ft Collins, CO 80523 USA. [Nol, Pauline] USDA, Natl Wildlife Res Ctr, Vet Serv, Anim & Plant Hlth Inspect Serv, Washington, DC USA. [Sonne, Christian] Aarhus Univ, Fac Sci & Technol, Dept Biosci, ARC, DK-8000 Aarhus C, Denmark. [Laidre, Kristin] Univ Washington, Polar Sci Ctr, Appl Phys Lab, Seattle, WA 98195 USA. [Obbard, Martyn] Ontario Minist Nat Resources & Forestry, Wildlife Res & Monitoring Sect, Toronto, ON, Canada. [Wiig, Oystein] Univ Oslo, Nat Hist Museum, N-0316 Oslo, Norway. [Aars, Jon] Norwegian Polar Res Inst, Oslo, Norway. [Regehr, Eric] US Fish & Wildlife Serv, Marine Mammals Management Program, Falls Church, VA USA. [Atwood, Todd] US Geol Survey, Alaska Sci Ctr, Washington, DC USA. RP Patyk, KA (reprint author), 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Kelly.A.Patyk@aphis.usda.gov; tatwood@usgs.gov RI Sonne, Christian/I-7532-2013; Wiig, Oystein/J-8383-2012; OI Sonne, Christian/0000-0001-5723-5263; Wiig, Oystein/0000-0003-0395-5251; Obbard, Martyn/0000-0003-2064-0155 NR 51 TC 6 Z9 6 U1 10 U2 94 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 1 PY 2015 VL 514 BP 371 EP 378 DI 10.1016/j.scitotenv.2015.02.007 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CE7RM UT WOS:000352039500041 PM 25679818 ER PT J AU Zhang, YT Wang, HY Liu, S Lei, QL Liu, J He, JQ Zhai, LM Ren, TZ Liu, HB AF Zhang, Yitao Wang, Hongyuan Liu, Shen Lei, Qiuliang Liu, Jian He, Jianqiang Zhai, Limei Ren, Tianzhi Liu, Hongbin TI Identifying critical nitrogen application rate for maize yield and nitrate leaching in a Haplic Luvisol soil using the DNDC model SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Maize; Yield; Amount of nitrate leaching; DNDC; Critical nitrogen application rate ID NORTH CHINA PLAIN; ROW-CROP FIELD; RAINFALL EVENTS; OXIDE EVOLUTION; ROOT-ZONE; MANAGEMENT; SYSTEMS; WHEAT; WATER; GROUNDWATER AB Identification of critical nitrogen (N) application rate can provide management supports for ensuring grain yield and reducing amount of nitrate leaching to ground water. A five-year (2008-2012) field lysimeter (1 m x 2 m x 1.2 m) experiment with three N treatments (0,180 and 240 kg N ha(-1)) was conducted to quantify maize yields and amount of nitrate leaching from a Haplic Luvisol soil in the North China Plain. The experimental data were used to calibrate and validate the process-based model of Denitrification-Decomposition (DNDC). After this, the model was used to simulate maize yield production and amount of nitrate leaching under a series of N application rates and to identify critical N application rate based on acceptable yield and amount of nitrate leaching for this cropping system. The results of model calibration and validation indicated that the model could correctly simulate maize yield and amount of nitrate leaching, with satisfactory values of RMSE-observation standard deviation ratio, model efficiency and determination coefficient. The model simulations confirmed the measurements that N application increased maize yield compared with the control, but the high N rate (240 kg N ha(-1)) did not produce more yield than the low one (120 kg N ha(-1)), and that the amount of nitrate leaching increased with increasing N application rate. The simulation results suggested that the optimal N application rate was in a range between 150 and 240 kg ha(-1), which would keep the amount of nitrate leaching below 18.4 kg NO3--N ha(-1) and meanwhile maintain acceptable maize yield above 9410 kg ha(-1). Furthermore, 180 kg N ha(-1) produced the highest yields (9837 kg ha(-1)) and comparatively lower amount of nitrate leaching (10.0 kg NO3--N ha(-1)). This study will provide a valuable reference for determining optimal N application rate (or range) in other crop systems and regions in China. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhang, Yitao; Wang, Hongyuan; Liu, Shen; Lei, Qiuliang; Zhai, Limei; Liu, Hongbin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Nonpoint Source Pollut Control, Minist Agr, Beijing 100081, Peoples R China. [Liu, Jian] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [He, Jianqiang] Northwest A&F Univ, Key Lab Agr Soil & Water Engn Arid & Semiarid Are, Minist Educ, Yangling 712100, Shaanxi, Peoples R China. [Ren, Tianzhi] Minist Agr, Inst Agroenvironm Protect, Tianjin 300191, Peoples R China. RP Liu, HB (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Nonpoint Source Pollut Control, Minist Agr, Beijing 100081, Peoples R China. EM liuhongbin@caas.cn RI Liu, Jian/P-4050-2014 OI Liu, Jian/0000-0003-4199-1296 FU Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture, China [201003014]; National Natural Science Foundation of China [41301311] FX This research was supported by the Special Fund for Agro-scientific Research in the Public Interest from the Ministry of Agriculture, China (Grant No.: 201003014) and the National Natural Science Foundation of China (Grant No.:41301311). We sincerely thank Mr. Bo Yang for his substantial help in field experiment management. NR 42 TC 4 Z9 4 U1 8 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 1 PY 2015 VL 514 BP 388 EP 398 DI 10.1016/j.scitotenv.2015.02.022 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CE7RM UT WOS:000352039500043 PM 25681775 ER PT J AU LaFrentz, BR Shoemaker, CA AF LaFrentz, Benjamin R. Shoemaker, Craig A. TI Passive transfer of serum from tilapia vaccinated with a Vibrio vulnificus vaccine provides protection from specific pathogen challenge SO AQUACULTURE LA English DT Article DE Vibrio vulnificus; Tilapia; Passive immunization; Antibody-mediated protection ID ANGUILLA-ANGUILLA; EUROPEAN EEL; SEROVAR-E; BIOTYPE-2; IDENTIFICATION; IMMUNIZATION; PROFILES; ANTIBODY; L. AB Vibrio vulnificus is a Gram-negative bacterium that has been associated with disease losses in some aquaculture reared fish species. Vaccination has proven effective for reducing the impact of this disease and research has suggested that specific antibodies are important for protective immunity. The present study determined the role of antibodies specific for V. vulnificus in protection by passive immunization and identified components of the bacterium the antibodies specifically recognize. Antiserum was generated by vaccinating hybrid tilapia with a formalin killed V. vulnificus ARS-1-Br-09 bacterin. Two passive immunization experiments were conducted, with and without heat inactivation of the antiserum. In both experiments, hybrid tilapia (mean weight, 6.5 g) were passively immunized by intraperitoneal injection of antiserum or control serum and then challenged with homologous V. vulnificus 24 h post-immunization. Following the challenge, relative percent survival values of 86 and 90 were obtained for tilapia passively immunized with non-heated and heat inactivated antiserum, respectively. Cell lysates and lipopolysaccharide preparations from V. vulnificus ARS-1-Br-09 and a heterologous isolate (CECT 4601) were probed with the antiserum and control serum by western blot analyses to determine the specificity of the antibodies. The antibodies exhibited specificity to proteins of both isolates and to the lipopolysaccharide of only the homologous isolate. The results supported a role of specific antibodies in the protection of tilapia against V. vulnificus, and suggested that shared immunogenic antigens were involved in protection previously described against heterologous isolates. Published by Elsevier B.V. C1 [LaFrentz, Benjamin R.; Shoemaker, Craig A.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP LaFrentz, BR (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM benjamin.lafrentz@ars.usda.gov FU USDA-ARS CRIS Project [6420-32000-024-00D] FX The authors acknowledge the technical support of Paige Mumma and Ning Qin. This research was supported by the USDA-ARS CRIS Project No. 6420-32000-024-00D (Integrated Aquatic Animal Health Strategies). The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 22 TC 0 Z9 0 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD MAY 1 PY 2015 VL 442 BP 16 EP 20 DI 10.1016/j.aquaculture.2015.02.025 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CE4YS UT WOS:000351837100003 ER PT J AU Showler, AT AF Showler, Allan T. TI Effects of compost and chicken litter on soil nutrition, and sugarcane physiochemistry, yield, and injury caused by Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae) SO CROP PROTECTION LA English DT Article DE Chemical ecology; Carbohydrates; Free amino acids; Nitrogen; Nutrition; Organic matter; Saccharum; Soil; Sugar ID LARVAL FEEDING PREFERENCES; SPODOPTERA-EXIGUA HUBNER; GOSSYPIUM-HIRSUTUM L.; BEET ARMYWORM; OVIPOSITION PREFERENCE; ELDANA-SACCHARINA; GROWTH-RESPONSES; PLANT NITROGEN; AMINO-ACIDS; ROOT-ROT AB Soil enrichment with two rates of compost and chicken litter were compared with conventionally fertilized sugarcane, Saccharum spp., in the subtropical Lower Rio Grande Valley of south Texas for two consecutive seasons. The high compost rate resulted in the most consistent increases of soil nutrients, including N, P, K, Ca, Mg, S, Fe, Zn, Mn, and Cu. Analysis of nutrients in sugarcane leaf tissue showed that only N was greater in the high compost treatment, and this was associated with greater abundances of three free amino acids, arginine, histidine, and lysine (each essential to insect growth and development), and of the sugar fructose. Although the high compost treatment yielded the most marketable sugarcane stalks per stool during the first season, this benefit was offset by greater infestations by the Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), in terms of numbers of entry and exit holes per stalk, and percentages of injured internodes and stalks. As a result, stalk weight, length, and percentage brix were reduced. During the second season, numbers of stalks per stool were not greater in the high compost treatment, nor were brix percentages less, but stalk weight and length were decreased and Mexican rice borer injury was greatly increased. This study demonstrates that changes in host plant biochemistry resulting from enhanced soil nutritional quality can have substantial negative effects on sugarcane production in the presence of Mexican rice borers. Hence, soil quality should be considered as part of integrated efforts to manage the pest. Published by Elsevier Ltd. C1 USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Showler, AT (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM allan.showler@ars.usda.gov NR 83 TC 4 Z9 4 U1 5 U2 25 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAY PY 2015 VL 71 BP 1 EP 11 DI 10.1016/j.cropro.2015.01.020 PG 11 WC Agronomy SC Agriculture GA CE4OR UT WOS:000351810400001 ER EF