FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Bruckart, WL Eskandari, FM Lane, WA AF Bruckart, W. L., III Eskandari, F. M. Lane, W. A. TI First Report of Leaf Necrosis on Microstegium vimineum Caused by Bipolaris microstegii in Maryland SO PLANT DISEASE LA English DT News Item C1 [Bruckart, W. L., III; Eskandari, F. M.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Lane, W. A.] Hood Coll, Frederick, MD 21701 USA. RP Bruckart, WL (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. NR 2 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2014 VL 98 IS 6 BP 852 EP 852 DI 10.1094/PDIS-11-13-1122-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AH3EB UT WOS:000336005200051 ER PT J AU Stanghellini, ME Mohammadi, M Mathews, DM Adkins, S AF Stanghellini, M. E. Mohammadi, M. Mathews, D. M. Adkins, S. TI First Report of Vine Decline of Mature Watermelon Plants Caused by Olpidium bornovanus SO PLANT DISEASE LA English DT News Item C1 [Stanghellini, M. E.; Mohammadi, M.; Mathews, D. M.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Adkins, S.] USDA ARS, Ft Pierce, FL 34945 USA. RP Stanghellini, ME (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. NR 3 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2014 VL 98 IS 6 BP 852 EP 852 DI 10.1094/PDIS-11-13-1123-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AH3EB UT WOS:000336005200052 ER PT J AU Wolfenbarger, SN Eck, EB Ocamb, CM Probst, C Nelson, ME Grove, GG Gent, DH AF Wolfenbarger, S. N. Eck, E. B. Ocamb, C. M. Probst, C. Nelson, M. E. Grove, G. G. Gent, D. H. TI Powdery Mildew Outbreaks caused by Podosphaera macularis on Hop Cultivars Possessing the Resistance Gene R6 in the Pacific Northwestern United States SO PLANT DISEASE LA English DT News Item C1 [Wolfenbarger, S. N.; Eck, E. B.; Ocamb, C. M.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Probst, C.; Nelson, M. E.; Grove, G. G.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Wolfenbarger, SN (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. NR 4 TC 3 Z9 3 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2014 VL 98 IS 6 BP 852 EP 853 DI 10.1094/PDIS-11-13-1127-PDN PG 2 WC Plant Sciences SC Plant Sciences GA AH3EB UT WOS:000336005200053 ER PT J AU Demers, JE McKemy, JM Bushe, B Conant, P Kumashira, B Ko, M Castlebury, LA AF Demers, J. E. McKemy, J. M. Bushe, B. Conant, P. Kumashira, B. Ko, M. Castlebury, L. A. TI First Report of Rust Caused by Pucciniastrum boehmeriae on Mamaki (Pipturus albidus) in Hawaii SO PLANT DISEASE LA English DT News Item C1 [Demers, J. E.; Castlebury, L. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [McKemy, J. M.] USDA APHIS PPQ Natl Identificat Serv, Beltsville, MD 20705 USA. [Bushe, B.] Univ Hawaii Manoa, Agr Diagnost Serv Ctr, Hilo, HI 96720 USA. [Conant, P.] Hawaii Dept Agr, Hilo, HI 96720 USA. [Kumashira, B.; Ko, M.] Hawaii Dept Agr, Honolulu, HI 96814 USA. RP Demers, JE (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. NR 4 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2014 VL 98 IS 6 BP 855 EP 855 DI 10.1094/PDIS-11-13-1172-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AH3EB UT WOS:000336005200059 ER PT J AU Trigiano, RN Rinehart, TA Dee, MM Wadl, PA Poplawski, L Ownley, BH AF Trigiano, R. N. Rinehart, T. A. Dee, M. M. Wadl, P. A. Poplawski, L. Ownley, B. H. TI First Report of Aerial Blight of Ruth's Golden Aster (Pityopsis ruthii) Caused by Rhizoctonia solani in the United States SO PLANT DISEASE LA English DT News Item C1 [Trigiano, R. N.; Dee, M. M.; Wadl, P. A.; Poplawski, L.; Ownley, B. H.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Rinehart, T. A.] USDA ARS, Thad Chcoran Southern Hort Lab, Poplarville, MS 39470 USA. RP Trigiano, RN (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. NR 2 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2014 VL 98 IS 6 BP 855 EP 856 DI 10.1094/PDIS-11-13-1181-PDN PG 2 WC Plant Sciences SC Plant Sciences GA AH3EB UT WOS:000336005200060 ER PT J AU Byun, AJ Hung, KE Fleet, JC Bronson, RT Mason, JB Garcia, PE Crott, JW AF Byun, Alexander J. Hung, Kenneth E. Fleet, James C. Bronson, Roderick T. Mason, Joel B. Garcia, Paloma E. Crott, Jimmy W. TI Colon-specific tumorigenesis in mice driven by Cre-mediated inactivation of Apc and activation of mutant Kras SO CANCER LETTERS LA English DT Article DE Colorectal cancer; Apc; Mouse model; Tumor; Adenoma; Kras ID MULTIPLE INTESTINAL NEOPLASIA; MOUSE MODELS; COLORECTAL-CANCER; PROGRESSION; CARCINOGENESIS; EXPRESSION; MUTATION; TUMORS; GENE AB Several genetically engineered mouse (GEM) models of colorectal cancer have been developed and are a mainstay in our efforts to identify means of preventing and treating this disease. Many of these models involve a germline disruption of the adenomatous polyposis coli (Apc) tumor suppressor gene and share the limitation that the great preponderance of tumors appear in the small rather than large intestine. In recent years efforts have been made to increase the similarity of these models to human sporadic colorectal cancer by disrupting Apc in a tissue-specific fashion using the Cre-Lox system so that the genetic aberrations are confined to the colonic epithelium. These models have shown great promise but reproducible and high penetrance colon-specific tumorigenesis has not yet been achieved without invasive techniques to introduce the Cre enzyme. We therefore sought to create a new model with high penetrance colon-specific tumorigenesis but without the need for exogenous Cre administration. We utilized existing mice possessing a conditional knock out for the Apc gene and a latent activated Kras allele and crossed them with mice expressing Cre recombinase solely in the large intestine. Using this approach we generated mice that developed 1-9 colonic adenomas per mouse (average 4.3) but without any tumors in the small intestine or cecum. No invasive tumors were observed. Despite the apparent lack of invasion, the geographical correctness, complete penetrance and intermediate tumor burden make this model a promising addition to our toolkit for the study of colorectal cancer treatment and prevention. (C) 2014 Elsevier Ireland Ltd. All rights reserved. C1 [Byun, Alexander J.; Mason, Joel B.; Garcia, Paloma E.; Crott, Jimmy W.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Vitamins & Carcinogenesis Lab, Boston, MA 02111 USA. [Hung, Kenneth E.] Pfizer, Clin Res & Dev, Biotherapeut, Cambridge, MA USA. [Fleet, James C.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Bronson, Roderick T.] Harvard Univ, Sch Med, Rodent Histopathol Core, Boston, MA USA. RP Crott, JW (reprint author), Tufts Univ, HNRCA, 711 Washington St, Boston, MA 02111 USA. EM jimmy.crott@tufts.edu OI Fleet, James/0000-0002-0142-9517 FU U.S. Department of Agriculture [8-1950-7-707] FX This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-7-707. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Dept of Agriculture. NR 19 TC 7 Z9 7 U1 0 U2 5 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0304-3835 EI 1872-7980 J9 CANCER LETT JI Cancer Lett. PD JUN 1 PY 2014 VL 347 IS 2 BP 191 EP 195 DI 10.1016/j.canlet.2014.03.004 PG 5 WC Oncology SC Oncology GA AG7WJ UT WOS:000335629200005 PM 24632531 ER PT J AU Whiteley, AR McGarigal, K Schwartz, MK AF Whiteley, Andrew R. McGarigal, Kevin Schwartz, Michael K. TI Pronounced differences in genetic structure despite overall ecological similarity for two Ambystoma salamanders in the same landscape SO CONSERVATION GENETICS LA English DT Article DE Genetic structure; Full-sibling families; Ambystoma; Effective number of breeders; Life-history ID EFFECTIVE POPULATION-SIZE; MICROSATELLITE DNA MARKERS; POND-BREEDING AMPHIBIANS; SPOTTED SALAMANDERS; LINKAGE DISEQUILIBRIUM; FRAGMENTED LANDSCAPE; CONSERVATION; MACULATUM; FLOW; EMIGRATION AB Studies linking genetic structure in amphibian species with ecological characteristics have focused on large differences in dispersal capabilities. Here, we test whether two species with similar dispersal potential but subtle differences in other ecological characteristics also exhibit strong differences in genetic structure in the same landscape. We examined eight microsatellites in marbled salamanders (Ambystoma opacum) from 29 seasonal ponds and spotted salamanders (Ambystoma maculatum) from 19 seasonal ponds in a single geographic region in west-central Massachusetts. Despite overall similarity in ecological characteristics of spotted and marbled salamanders, we observed clear differences in the genetic structure of these two species. For marbled salamanders, we observed strong overall genetic differentiation (F (ST) = 0.091, F' (ST) = 0.375), three population-level clusters of populations (K = 3), a strong pattern of isolation by distance (r = 0.58), and marked variation in family-level structure (from 1 to 23 full-sibling families per site). For spotted salamanders, overall genetic differentiation was weaker (F (ST) = 0.025, F' (ST) = 0.102), there was no evidence of population-level clustering (K = 1), the pattern of isolation by distance (r = 0.17) was much weaker compared to marbled salamanders, and there was less variation in family-level structure (from 10 to 36 full-sibling families per site). We suspect that a combination of breeding site fidelity, effective population size, and generation interval is responsible for these marked differences. Our results suggest that marbled salamanders, compared to spotted salamanders, are more sensitive to fragmentation from various land-use activities and would be less likely to recolonize extirpated sites on an ecologically and conservation-relevant time frame. C1 [Whiteley, Andrew R.; McGarigal, Kevin] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Whiteley, Andrew R.] Univ Massachusetts, US Forest Serv, No Res Stn, Amherst, MA 01003 USA. [Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. RP Whiteley, AR (reprint author), Univ Massachusetts, US Forest Serv, No Res Stn, Amherst, MA 01003 USA. EM awhiteley@eco.umass.edu RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 NR 80 TC 11 Z9 11 U1 4 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD JUN PY 2014 VL 15 IS 3 BP 573 EP 591 DI 10.1007/s10592-014-0562-7 PG 19 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AG8MB UT WOS:000335671500007 ER PT J AU Svejcar, T Boyd, C Davies, K Madsen, M Bates, J Sheley, R Marlow, C Bohnert, D Borman, M Mata-Gonzalez, R Buckhouse, J Stringham, T Perryman, B Swanson, S Tate, K George, M Ruyle, G Roundy, B Call, C Jensen, K Launchbaugh, K Gearhart, A Vermeire, L Tanaka, J Derner, J Frasier, G Havstad, K AF Svejcar, Tony Boyd, Chad Davies, Kirk Madsen, Matthew Bates, Jon Sheley, Roger Marlow, Clayton Bohnert, David Borman, Mike Mata-Gonzalez, Ricardo Buckhouse, John Stringham, Tamzen Perryman, Barry Swanson, Sherman Tate, Kenneth George, Mel Ruyle, George Roundy, Bruce Call, Chris Jensen, Kevin Launchbaugh, Karen Gearhart, Amanda Vermeire, Lance Tanaka, John Derner, Justin Frasier, Gary Havstad, Kris TI Western Land Managers will Need all Available Tools for Adapting to Climate Change, Including Grazing: A Critique of Beschta et al. SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Grazing; Public lands; Climate change; Riparian areas AB In a previous article, Beschta et al. (Environ Manag 51(2):474-491, 2013) argue that grazing by large ungulates (both native and domestic) should be eliminated or greatly reduced on western public lands to reduce potential climate change impacts. The authors did not present a balanced synthesis of the scientific literature, and their publication is more of an opinion article. Their conclusions do not reflect the complexities associated with herbivore grazing. Because grazing is a complex ecological process, synthesis of the scientific literature can be a challenge. Legacy effects of uncontrolled grazing during the homestead era further complicate analysis of current grazing impacts. Interactions of climate change and grazing will depend on the specific situation. For example, increasing atmospheric CO2 and temperatures may increase accumulation of fine fuels (primarily grasses) and thus increase wildfire risk. Prescribed grazing by livestock is one of the few management tools available for reducing fine fuel accumulation. While there are certainly points on the landscape where herbivore impacts can be identified, there are also vast grazed areas where impacts are minimal. Broad scale reduction of domestic and wild herbivores to help native plant communities cope with climate change will be unnecessary because over the past 20-50 years land managers have actively sought to bring populations of native and domestic herbivores in balance with the potential of vegetation and soils. To cope with a changing climate, land managers will need access to all available vegetation management tools, including grazing. C1 [Svejcar, Tony; Boyd, Chad; Davies, Kirk; Madsen, Matthew; Bates, Jon; Sheley, Roger] ARS, USDA, Burns, OR 97720 USA. [Marlow, Clayton] Montana State Univ, Bozeman, MT 59717 USA. [Bohnert, David] Oregon State Univ, Burns, OR 97720 USA. [Borman, Mike; Mata-Gonzalez, Ricardo; Buckhouse, John] Oregon State Univ, Corvallis, OR 97331 USA. [Stringham, Tamzen; Perryman, Barry; Swanson, Sherman] Univ Nevada, Reno, NV 89557 USA. [Tate, Kenneth; George, Mel] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Ruyle, George] Univ Arizona, Tucson, AZ 85721 USA. [Roundy, Bruce] Brigham Young Univ, Provo, UT 84602 USA. [Call, Chris] Utah State Univ, Logan, UT 84322 USA. [Jensen, Kevin] Utah State Univ, ARS, USDA, Logan, UT 84322 USA. [Launchbaugh, Karen] Univ Idaho, Moscow, ID 83844 USA. [Gearhart, Amanda] Univ Idaho, Twin Falls, ID 83303 USA. [Vermeire, Lance] ARS, USDA, Miles City, MT 59301 USA. [Tanaka, John] Univ Wyoming, Laramie, WY 82071 USA. [Derner, Justin] ARS, USDA, Cheyenne, WY 82009 USA. [Frasier, Gary] ARS, USDA, Ft Collins, CO 80526 USA. [Havstad, Kris] ARS, USDA, Las Cruces, NM 88003 USA. RP Svejcar, T (reprint author), ARS, USDA, 67826-A Hwy 205, Burns, OR 97720 USA. EM tony.svejcar@oregonstate.edu; cmarlow@montana.edu NR 21 TC 4 Z9 4 U1 1 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD JUN PY 2014 VL 53 IS 6 BP 1035 EP 1038 DI 10.1007/s00267-013-0218-2 PG 4 WC Environmental Sciences SC Environmental Sciences & Ecology GA AG8JC UT WOS:000335663600001 PM 24399203 ER PT J AU Graham, JH Gottwald, TR Timmer, LW Bergamin, A Van den Bosch, F Irey, MS Taylor, E Magarey, RD Takeuchi, Y AF Graham, James H. Gottwald, Tim R. Timmer, Lavern W. Bergamin Filho, Armando Van den Bosch, Frank Irey, Michael S. Taylor, Earl Magarey, Roger D. Takeuchi, Yu TI Response to "Potential distribution of citrus black spot in the United States based on climatic conditions", Er et al. 2013 SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Editorial Material ID GUIGNARDIA-CITRICARPA C1 [Graham, James H.; Timmer, Lavern W.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Gottwald, Tim R.; Taylor, Earl] USDA ARS US, Hort Res Lab, Ft Pierce, FL 34945 USA. [Van den Bosch, Frank] Rothamsted Res Computat & Syst Biol, Harpenden AL5 2JQ, Herts, England. [Bergamin Filho, Armando] Univ Sao Paulo, ESALQ, Piracicaba, Brazil. [Irey, Michael S.] US Sugar Corp, Res Dept, Clewiston, FL 33440 USA. [Magarey, Roger D.; Takeuchi, Yu] N Carolina State Univ, Ctr Integrated Pest Management, Raleigh, NC 27606 USA. RP Graham, JH (reprint author), Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM jhgraham@ufl.edu NR 5 TC 3 Z9 3 U1 0 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUN PY 2014 VL 139 IS 2 BP 231 EP 234 DI 10.1007/s10658-014-0426-5 PG 4 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AG6AR UT WOS:000335501200001 ER PT J AU Chronis, D Chen, SY Skantar, AM Zasada, IA Wang, XH AF Chronis, Demosthenis Chen, Shiyan Skantar, Andrea M. Zasada, Inga A. Wang, Xiaohong TI A new chorismate mutase gene identified from Globodera ellingtonae and its utility as a molecular diagnostic marker SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Alternative splicing; Chorismate mutase; Globodera ellingtonae; Molecular diagnosis; Potato cyst nematode; TaqMan qPCR ID POTATO CYST-NEMATODE; UNITED-STATES; PALLIDA; ROSTOCHIENSIS; BIOLOGY; REGION; OREGON; IDAHO AB Globodera ellingtonae, a new cyst nematode species recently detected in Oregon and confirmed to reproduce on potato, shares key morphological features with the two species of potato cyst nematode (PCN; G. rostochiensis and G. pallida) which are of quarantine concern. Currently no methods are available for the molecular diagnosis of this new Globodera species. In this study, we cloned a chorismate mutase gene (Ge-cm-1) from G. ellingtonae. Our detailed sequence analysis identified two different Ge-cm-1 mRNA transcripts, named as Ge-cm-1 and Ge-cm-1-IRII, of which Ge-cm-1-IRII differs from Ge-cm-1 by a 93-base pair (bp) insertion. Comparison of the Ge-cm-1-IRII transcript with the genomic sequence of Ge-cm-1 revealed that Ge-cm-1-IRII was an alternatively spliced transcript generated by intron retention, further confirming a previous discovery that alternative splicing of CM genes is conserved among Globodera species. The genomic sequence of Ge-cm-1 contains three introns with intron 1 showing significant divergence compared to those of CM genes from the two PCN species as well as a related species G. tabacum. Based on the sequence variations, we designed PCR primers and a TaqMan probe specific for Ge-cm-1 and developed a TaqMan qPCR assay that provides reliable and sensitive identification of G. ellingtonae. Due to the fact that multiple Globodera species that infect potato currently occur in the U.S., this new molecular diagnostic method is valuable and should be included with other standard diagnostic methods to achieve a rapid and accurate differentiation of Globodera species. C1 [Chronis, Demosthenis; Wang, Xiaohong] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Chen, Shiyan; Wang, Xiaohong] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Skantar, Andrea M.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. [Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Wang, XH (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM xiaohong.wang@ars.usda.gov RI Chen, Shiyan/N-1832-2014 FU USDA-ARS; USDA-APHIS FX We would like to thank Eric Grenier for providing DNA from Globodera populations from South Peru, Chile and UK, Geert Smant for providing DNA from PCN populations from the Netherlands, Solke De Boer for providing DNA from a G. pallida population from Newfoundland, Canada, Melissa Mitchum for providing G. tabacum, and Eric Davis for providing Heterodera glycines. This study was supported by funding from USDA-ARS and USDA-APHIS. NR 20 TC 2 Z9 3 U1 1 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUN PY 2014 VL 139 IS 2 BP 239 EP 246 DI 10.1007/s10658-014-0385-x PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AG6AR UT WOS:000335501200003 ER PT J AU Lee, IM Polashock, J Bottner-Parker, KD Bagadia, PG Rodriguez-Saona, C Zhao, Y Davis, RE AF Lee, I. -M. Polashock, J. Bottner-Parker, K. D. Bagadia, P. G. Rodriguez-Saona, C. Zhao, Y. Davis, R. E. TI New subgroup 16SrIII-Y phytoplasmas associated with false-blossom diseased cranberry (Vaccinium macrocarpon) plants and with known and potential insect vectors in New Jersey SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Blunt-nosed leafhopper Limotettix vaccinii (=Scleroracus vaccinii, Euscellis striatulus); Sharp-nosed leafhopper Scaphytopius magdalensis; iPhyClassifier ID POLYMERASE-CHAIN-REACTION; 16S RIBOSOMAL-RNA; MYCOPLASMALIKE ORGANISMS; X-DISEASE; CLASSIFICATION; PHYLOGENY AB The identity of the presumed phytoplasmal pathogen associated with cranberry false-blossom disease has never been fully clarified. In the present study a molecular-based procedure was employed to determine the identity of the phytoplasma. Tissues of cranberry plants exhibiting cranberry false-blossom symptoms were collected from multiple bogs on each of three randomly selected commercial cranberry farms in New Jersey. Leafhoppers, including the known vector Limotettix vaccinii (Van Duzee) (=Scleroracus vaccinii, Euscellis striatulus) and the sharp-nosed leafhopper Scaphytopius magdalensis (Provancher), a known vector of blueberry stunt disease, were collected from two different farms in New Jersey. Nested PCR assays and RFLP analysis of 16S rRNA gene sequences were employed for the detection and identification of the associated phytoplasmas. All of 20 cranberry plants sampled and five out of 14 batches of leafhoppers tested positive for phytoplasma. Virtual RFLP and sequence analyses revealed that all the associated phytoplasmas were members or variants of a new subgroup, 16SrIII-Y. Phylogenetic analysis of 16S rRNA sequences indicated that cranberry false-blossom phytoplasma strains represented a lineage distinct from other 16SrIII subgroups. This is the first report confirming that a new phytoplasma (designated as a new subgroup 16SrIII-Y) is associated with cranberry false-blossom disease and associated with both leafhopper species in New Jersey. C1 [Lee, I. -M.; Bottner-Parker, K. D.; Zhao, Y.; Davis, R. E.] Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Polashock, J.] USDA ARS, GIFVL, Chatsworth, NJ 08019 USA. [Bagadia, P. G.] Univ Maryland, College Pk, MD 20742 USA. [Rodriguez-Saona, C.] Rutgers State Univ, Philip E Marucci Blueberry & Cranberry Res Ctr, Chatsworth, NJ 08019 USA. RP Lee, IM (reprint author), Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM ingming.lee@ars.usda.gov NR 17 TC 3 Z9 3 U1 1 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUN PY 2014 VL 139 IS 2 BP 393 EP 400 DI 10.1007/s10658-014-0396-7 PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AG6AR UT WOS:000335501200017 ER PT J AU Coates, BS AF Coates, Brad S. TI Assembly and annotation of full mitochondrial genomes for the corn rootworm species, Diabrotica virgifera virgifera and Diabrotica barberi (Insecta: Coleoptera: Chrysomelidae), using Next Generation Sequence data SO GENE LA English DT Article DE Next Generation Sequencing; Full mitochondrial genome; Molecular divergence; Phylogenetics ID COMPOSITIONAL HETEROGENEITY; DNA; ORGANIZATION; DROSOPHILA; PHYLOGENY; EVOLUTION; GENES; LIFE; CONSERVATION; INSECTICIDES AB Complete mitochondrial genomes for two corn rootworm species, Diabrotica virgifera virgifera (16,747 bp) and Diabrotica barberi (16,632; Insecta: Coleoptera: Chrysomelidae), were assembled from Illumina HiSeq2000 read data. Annotation indicated that the order and orientation of 13 protein coding genes (PCGs), and,22 tRNA and 2 rRNA sequences were in typical of insect mitochondrial genomes. Non-standard nad4 and cox3 stop codons were composed of single T nucleotides and likely completed by adenylation, and atypical ITT start codons was predicted for both D. v. virgtfera and D. barberi nad1 genes. The D. v. vireera and D. barberi haplotypes showed 819 variable nucleotide positions within PCG regions (7.36% divergence), which suggest that speciation may have occurred similar to 3.68 million years ago assuming a linear rate of short-term substitution. Phylogenetic analyses of Coleopteran MID genome show clustering based on family level, and may have the capacity to resolve the evolutionary history within this Order of insects. Published by Elsevier B.V. C1 [Coates, Brad S.] Iowa State Univ, USDA ARS, Genet Lab 103, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Coates, Brad S.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. RP Coates, BS (reprint author), Iowa State Univ, USDA ARS, Genet Lab 103, Corn Insects & Crop Genet, Ames, IA 50011 USA. EM brad.coates@ars.usda.gov FU USDA, Agricultural Research Service (CRIS) [3625-22000-017-00D]; Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA [3543] FX This research was a joint contribution from the USDA, Agricultural Research Service (CRIS Project 3625-22000-017-00D), and the Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA (Project 3543). The author would like to thank B. Wade French from the United States Department of Agriculture-Agricultural Research Service, North Central Agricultural Research Laboratory (USDA-ARS, NCARL), Brookings, SD for providing samples from the non-diapausing D. v. virgifera strain, as well as USDA-ARS Technician, Robert Gunnarson, for performing DNA extraction protocols. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by USDA for their use. NR 55 TC 8 Z9 8 U1 1 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JUN 1 PY 2014 VL 542 IS 2 BP 190 EP 197 DI 10.1016/j.gene.2014.03.035 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA AG7YB UT WOS:000335633600014 PM 24657060 ER PT J AU Faris, JD Zhang, ZC Chao, SM AF Faris, Justin D. Zhang, Zengcui Chao, Shiaoman TI Map-based analysis of the tenacious glume gene Tg-B1 of wild emmer and its role in wheat domestication SO GENE LA English DT Article DE Triticum; Durum; Wheat evolution; Free-threshing ID TRITICUM-AESTIVUM L.; FREE-THRESHING HABIT; CHROMOSOME BIN MAP; HEXAPLOID WHEAT; BREAD WHEAT; TETRAPLOID WHEAT; POLYPLOID WHEAT; SPELTA L.; MOLECULAR CHARACTERIZATION; MICROSATELLITE MARKERS AB The domestication of wheat was instrumental in spawning the civilization of humankind, and it occurred through genetic mutations that gave rise to types with non-fragile rachises, soft glumes, and free-threshing seed. Wild emmer (Triticum turgidum ssp. dicoccoides), the tetraploid AB-genome progenitor of domesticated wheat has genes that confer tenacious glumes (Tg) that underwent genetic mutations to give rise to free-threshing wheat. Here, we evaluated disomic substitution lines involving chromosomes 2A and 2B of wild emmer accessions substituted for homologous chromosomes in tetraploid and hexaploid backgrounds. The results suggested that both chromosomes 2A and 2B of wild emmer possess genes that inhibit threshability. A population of recombinant inbred lines derived from the tetraploid durum wheat variety Langdon crossed with a Langdon T. turgidum ssp. dicoccoides accession P1481521 chromosome 2B disomic substitution line was used to develop a genetic linkage map of 2B, evaluate the genetics of threshability, and map the gene derived from P1481521 that inhibited threshability. A 2BS linkage map comprised of 58 markers was developed, and markers delineated the gene to a 2.3 cM interval. Comparative analysis with maps containing the tenacious glume gene Tg-D1 on chromosome arm 2DS from Aegilops tauschii, the D genome progenitor of hexaploid wheat, revealed that the gene inhibiting threshability in wild emmer was homoeologous to Tg-D1 and therefore designated Tg-B1. Comparative analysis with rice and Brachypodium distachyon indicated a high level of divergence and poorly conserved colinearity, particularly near the Tg-B1 locus. These results provide a foundation for further studies involving Tg-B1, which, together with Tg-D1, had profound influences on wheat domestication. Published by Elsevier B.V. C1 [Faris, Justin D.; Zhang, Zengcui; Chao, Shiaoman] USDA ARS, Red River Valley Agr Res Ctr, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Faris, JD (reprint author), USDA ARS, NPA, NCSL, 1605 Albrecht BLVD, Fargo, ND 58102 USA. EM Justin.Faris@ars.usda.gov FU USDA-ARS CRIS project [5442-22000-037-00D] FX The authors thank Ms. Breanna Oldenburg for threshing all spikes and collecting all of the phenotypic data for this study. This research was supported by USDA-ARS CRIS project 5442-22000-037-00D. NR 69 TC 7 Z9 8 U1 7 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JUN 1 PY 2014 VL 542 IS 2 BP 198 EP 208 DI 10.1016/j.gene.2014.03.034 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA AG7YB UT WOS:000335633600015 PM 24657062 ER PT J AU Kharel, K Arthur, FH Zhu, KY Campbell, JF Subramanyam, B AF Kharel, Kabita Arthur, Frank H. Zhu, Kun Yan Campbell, James F. Subramanyam, Bhadriraju TI Susceptibility of different life stages of Tribolium confusum to pyrethrin aerosol: effects of a flour food source on insecticidal efficacy SO JOURNAL OF PEST SCIENCE LA English DT Article DE Tribolium confusum; Life stages; Synergized pyrethrin; Aerosol; Flour mill; Sanitation ID STORED-PRODUCT INSECTS; CASTANEUM; MILL; TENEBRIONIDAE; COLEOPTERA; DYNAMICS; CONCRETE; IMPACT AB Accumulated grain dust and flour residues in flour mills can potentially decrease the efficacy of contact surface treatments and aerosol insecticides used for control of adult and immature stages of stored product insects. A study was conducted to evaluate the effects of flour residues on the efficacy of piperonyl butoxide-synergized pyrethrin aerosol against different life stages of the confused flour beetle, Tribolium confusum Jacquelin du Val. Twenty individual adults, pupae, or larvae of T. confusum, confined in Petri dishes containing 0, 0.1, 1, 5, or 10 g of wheat flour, were exposed separately inside an empty shed to the label rate of the aerosol. The formulation contained 1 % active ingredient pyrethrin and the label application rate was 1.04 ml/m(3). After 2 h of exposure, all dishes were removed from the shed and placed in an incubator set at 27 A degrees C and 60 % RH. Mortality of T. confusum adults decreased with increasing amount of flour. Recovery of moribund adults 15 days after exposure was greater in the 5 and 10 g flour dishes (15 and 46 %) compared to the 0.1 or 1 g flour dishes (0.7 and 5 %). As the flour amounts increased, more larvae and pupae were able to emerge as adults. Results show accumulated flour residues during aerosol application can compromise aerosol efficacy, and also suggest the flour impaired penetration ability of the aerosol. These results also emphasize the importance of sanitation and cleaning to remove spillage and extraneous material prior to an aerosol application. C1 [Kharel, Kabita; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Arthur, Frank H.; Campbell, James F.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. [Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RP Arthur, FH (reprint author), ARS, Ctr Grain & Anim Hlth Res, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. EM frank.arthur@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA), Methyl Bromide Transitions program [2010-51102-21660] FX We thank Brian Barnett, Rich Hammel, and Kris Hartzer for their technical assistance with the research and Entech Corporation for providing the insecticide used in this study. This research was partially funded by the U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA), Methyl Bromide Transitions program (grant number 2010-51102-21660). Insect voucher specimens were deposited at the Kansas State University Museum of Entomological and Prairie Arthropod Research under voucher number 226. This paper is contribution number 14-058-J of the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or by Kansas State University. USDA and Kansas State University are equal opportunity providers and employers. NR 21 TC 8 Z9 8 U1 2 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2014 VL 87 IS 2 BP 295 EP 300 DI 10.1007/s10340-013-0549-z PG 6 WC Entomology SC Entomology GA AG7CI UT WOS:000335575200006 ER PT J AU Medeiros, ES Offeman, RD Klamczynski, AP Glenn, GM Mattoso, LHC Orts, WJ AF Medeiros, Eliton S. Offeman, Richard D. Klamczynski, Artur P. Glenn, Gregory M. Mattoso, Luiz H. C. Orts, William J. TI Synthesis, Characterization and Nanocomposite Formation of Poly(glycerol succinate-co-maleate) with Nanocrystalline Cellulose SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Glycerol polymers; Nanocrystalline cellulose; Nanowhiskers; Microfibrils; Nanocomposites; Succinic acid; Polycondensation; Biodegradation ID CURRENT INTERNATIONAL RESEARCH; NYLON 6-CLAY HYBRID; POLYMER NANOCOMPOSITES; POLYSACCHARIDE NANOCRYSTALS; MECHANICAL-PROPERTIES; BIODIESEL PRODUCTION; CLAY NANOCOMPOSITES; WHEAT-STRAW; WHISKERS; COMPOSITES AB A novel biodegradable polymer based on glycerol, succinic anhydride and maleic anhydride, poly(glycerol succinate-co-maleate), poly(GlySAMA), was synthesized by melt polycondensation and tested as a matrix for composites with nanocrystalline cellulose. This glycerol-based polymer is thermally stable as a consequence of its targeted cross-linked structure. To broaden its range of properties, it was specifically formulated with nanocrystalline cellulose (NCC) at concentrations of 1, 2 and 4 wt%, and showed improved mechanical properties with NCC. Specifically, the effect of reinforcement on mechanical properties, thermal stability, structure, and biodegradability was evaluated, respectively, by tensile tests and thermogravimetric analyses, X-ray diffraction and respirometry. The neat poly(GlySAMA) polymer proved flexible, exhibiting an elongation-to-break of 8.8 % while the addition of nanowhiskers (at 4 wt%) caused tensile strength and Young's modulus to increase, 20 and 40 %, respectively. Stiffness improved without significantly decreasing thermal stability as measured by thermogravimetric analysis. Biodegradation tests indicated that all samples were degradable but NCC reduced the rate of biodegradation. C1 [Medeiros, Eliton S.] Univ Fed Paraiba UFPB, Dept Engn Mat DEMat, BR-58051900 Joao Pessoa, Paraiba, Brazil. [Offeman, Richard D.; Klamczynski, Artur P.; Glenn, Gregory M.; Orts, William J.] USDA, WRRC, Albany, CA 94710 USA. [Mattoso, Luiz H. C.] Embrapa Instrumentacao Agr, Lab Nacl Nanotecnol Aplicada Ao Agronegocio LNNA, BR-13560970 Sao Carlos, SP, Brazil. RP Orts, WJ (reprint author), USDA, WRRC, 800 Buchanan St, Albany, CA 94710 USA. EM bill.orts@ars.usda.gov RI Mattoso, Luiz H C/D-2794-2016 OI Mattoso, Luiz H C/0000-0001-7586-1014 FU CNPq (Brazilian research council) FX Authors are indebted to Lennard Torres for his assistance with polymerization and Dr. Colleen McMahan for providing her particle size analyzer. Thanks are also due to CNPq (Brazilian research council) for partly supporting this research. NR 44 TC 5 Z9 5 U1 4 U2 50 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD JUN PY 2014 VL 22 IS 2 BP 219 EP 226 DI 10.1007/s10924-014-0643-1 PG 8 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA AG9GW UT WOS:000335728400008 ER PT J AU Gustafson, LL Remmenga, MD Gardner, IA Hartman, KH Creekmore, LH Goodwin, AE Whaley, JE Warg, JV Gardner, SL Scott, AE AF Gustafson, L. L. Remmenga, M. D. Gardner, I. A. Hartman, K. H. Creekmore, L. H. Goodwin, A. E. Whaley, J. E. Warg, J. V. Gardner, S. L. Scott, A. E. TI Viral hemorrhagic septicemia IVb status in the United States: Inferences from surveillance activities and regional context SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Disease freedom; Aquatic animal health; Risk-based surveillance; Context-based surveillance; Viral hemorrhagic septicemia ID ESOX-MASQUINONGY MITCHILL; ST-LAWRENCE-RIVER; GREAT-LAKES; GENETIC DIVERSITY; VIRUS-INFECTION; GENOTYPE IVB; VHSV-IVB; ONTARIO; USA; TEMPERATURES AB The United States (U.S.) response to viral hemorrhagic septicemia virus (VHSV) IVb emergence in the Laurentian Great Lakes (GL) included risk-based surveillance for cost-effective decision support regarding the health of fish populations in open systems. All U.S. VHSV IVb isolations to date derive from free-ranging fish from GL States. Most originate in the region designated by US Geological Survey hydrologic unit code (HUC) 04, with the exception of two detections in neighboring Upper Mississippi (HUC 05) and Ohio (HUC 07) regions. For States outside the GL system, disease probability was assessed using multiple evidence sources. None substantiated VHSV IVb absence using surveillance alone, in part due to the limited temporal relevance of data in open systems. However, Bayesian odds risk-based analysis of surveillance and population context, coupled with exclusions where water temperatures likely preclude viral replication, achieved VHSV IVb freedom assurance for 14 non-GL States by the end of 2012, with partial evidence obtained for another 17 States. The non-GL region (defined as the aggregate of 4-digit HUCs located outside of GL States) met disease freedom targets for 2012 and is projected to maintain this status through 2016 without additional active surveillance. Projections hinge on continued basic biosecurity conditions such as movement restrictions and passive surveillance. Areas with navigable waterway connections to VHSV IVb-affected HUCs (and conducive water temperatures) should receive priority for resources in future surveillance or capacity building efforts. However, 6 years of absence of detections in non-GL States suggests that existing controls limit pathogen spread, and that even spread via natural pathways (e.g., water movement or migratory fish) appears contained to the Great Lakes system. This report exemplifies the cost-effective use of risk-based surveillance in decision support to assess and manage aquatic animal population health in open systems. Published by Elsevier B.V. C1 [Gustafson, L. L.; Remmenga, M. D.; Gardner, S. L.; Scott, A. E.] USDA, Ctr Epidemiol, Natl Surveillance Unit, Ft Collins, CO USA. [Gustafson, L. L.; Remmenga, M. D.; Gardner, S. L.; Scott, A. E.] USDA, Ctr Anim Hlth, Vet Serv, Anim & Plant Hlth Inspect Serv, Ft Collins, CO USA. [Gardner, I. A.] Univ Prince Edward Isl, Atlantic Vet Coll, Dept Hlth Management, Charlottetown, PE C1A 4P3, Canada. [Hartman, K. H.] USDA, Eastern Reg Off, Vet Serv, Anim & Plant Hlth Inspect Serv, Ruskin, FL USA. [Creekmore, L. H.] USDA, Western Reg Off, Vet Serv, Anim & Plant Hlth Inspect Serv, Ft Collins, CO USA. [Goodwin, A. E.] US Fish & Wildlife Serv, Pacific Reg, Portland, OR 97232 USA. [Whaley, J. E.] USDA, Aquaculture Program, Vet Serv, Anim & Plant Hlth Inspect Serv, Riverdale, MD USA. [Warg, J. V.] USDA, Diagnost Virol Lab, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, Ames, IA USA. RP Gustafson, LL (reprint author), 2150 Ctr Ave,Bldg B,Mail Stop 2E6, Ft Collins, CO 80526 USA. EM lori.l.gustafson@aphis.usda.gov FU Canada Excellence Research Chairs program FX This project was successful in large part due to stakeholder advice and support. We thank the many individuals representing State, Tribal Nation, laboratory, academic and industry cooperators who provided data, support and direction for this study. We also thank the affiliated agencies and industries, and the Canada Excellence Research Chairs program for supporting time and funds for these efforts. Finally, we thank the members of the VHSV Expert Panel for their continuing correspondence regarding questions related to VHSV IVb epidemiology and detections. NR 26 TC 3 Z9 4 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD JUN 1 PY 2014 VL 114 IS 3-4 BP 174 EP 187 DI 10.1016/j.prevetmed.2014.02.011 PG 14 WC Veterinary Sciences SC Veterinary Sciences GA AG7XV UT WOS:000335633000004 PM 24655577 ER PT J AU Grear, DA Kaneene, JB Averill, JJ Webb, CT AF Grear, Daniel A. Kaneene, John B. Averill, James J. Webb, Colleen T. TI Local cattle movements in response to ongoing bovine tuberculosis zonation and regulations in Michigan, USA SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Bovine tuberculosis; Mycobacterium bovis; Cattle movement; Michigan; Accreditation zone; Dynamic network ID NETWORK ANALYSIS; MYCOBACTERIUM-BOVIS; DISEASE-CONTROL; MOUTH-DISEASE; EPIDEMIC; FARMS; UK AB Bovine tuberculosis (Mycobacterium bovis) is an ongoing management issue in the state of Michigan with eradication from livestock as the ultimate goal. Eradication has been a challenge owing to the presence of a wildlife reservoir; competing interests in managing the livestock and wildlife hosts; and many uncertainties in transmission dynamics of M. bovis. One of the cornerstones of the eradication effort has been to stop movement of infected cattle among farms by imposing strict pre-movement testing on cattle being moved within, into and out of the Modified Accredited Zone (MAZ) in northeastern Michigan. In addition to pre-movement tuberculosis testing, detailed information about the origin and destination premises of all movements within the MAZ has been recorded in Michigan. The aim of this study was to describe the farm-to-farm movements of cattle within the MAZ, report changes in the network of movements during a 6-year period when the MAZ was a constant size (2004-2009), and examine changes in cattle movement patterns when the MAZ was reduced from 11 to 5 counties in 2010. Non-slaughter cattle movement within the MAZ was characterized by predominantly local movements at a sub-county scale. Premises that shipped cattle were primarily senders or receivers, but rarely both. From 2004 to 2009, the number of cattle shipped, size of shipments, number of shipments and distance of shipments decreased; there was little change in the network patterns of interaction among individual premises; and interactions among all premises became more disconnected. After accounting for MAZ size, there were also no changes in cattle movement network patterns following the reduction of the MAZ in 2010. The movement of cattle was likely not a key risk factor in bTB spread among premises in the MAZ during the study period and the effect of zonation and movement regulations appeared to further reduce the risk of tuberculosis spread via cattle movements among farms in Michigan's MAZ. Published by Elsevier B.V. C1 [Grear, Daniel A.; Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80526 USA. [Kaneene, John B.] Michigan State Univ, Ctr Comparat Epidemiol, E Lansing, MI 48824 USA. [Averill, James J.] Michigan Dept Agr & Rural Dev, Lansing, MI 48909 USA. RP Grear, DA (reprint author), USDA, APHIS, Ctr Epidemiol & Anim Hlth, Vet Serv, 2150 Ctr Ave Bldg B, Ft Collins, CO 80526 USA. EM daniel.a.grear@aphis.usda.gov OI Grear, Daniel/0000-0002-5478-1549 FU National Institute for Mathematical and Biological Synthesis (NIMBioS); Fogarty International Center - National Institutes of Health and Science and Technology Directorate - Department of Homeland Security; USDA [11-920-802-96CA]; U.S.D.A. FX Funding provided by the National Institute for Mathematical and Biological Synthesis (NIMBioS) for a bovine TB workshop and working group; Research and Policy for Infectious Disease Dynamics (RAPIDD) Program - Fogarty International Center - National Institutes of Health and Science and Technology Directorate - Department of Homeland Security, and USDA Cooperative Agreement 11-920-802-96CA. While funding was provided by the U.S.D.A., Animal and Plant Health Inspection Service, Veterinary Services, the analyses, views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the regulatory opinions, official policies, either expressed or implied, of the USDA-APHIS-Veterinary Services. We thank K. Portacci, P. Riggs and R.S. Miller for valuable input on early drafts. We also thank the state veterinarians and staff, whose cooperation and effort made the data collection possible. NR 32 TC 3 Z9 3 U1 0 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD JUN 1 PY 2014 VL 114 IS 3-4 BP 201 EP 212 DI 10.1016/j.prevetmed.2014.03.008 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA AG7XV UT WOS:000335633000006 PM 24685049 ER PT J AU Camacho, LE Lemley, CO Prezotto, LD Bauer, ML Freetly, HC Swanson, KC Vonnahme, KA AF Camacho, L. E. Lemley, C. O. Prezotto, L. D. Bauer, M. L. Freetly, H. C. Swanson, K. C. Vonnahme, K. A. TI Effects of maternal nutrient restriction followed by realimentation during midgestation on uterine blood flow in beef cows SO THERIOGENOLOGY LA English DT Article DE Beef cow; Nutrient restriction; Pregnancy; Uterine blood flow ID TRANSRECTAL DOPPLER SONOGRAPHY; FETAL-DEVELOPMENT; EARLY-PREGNANCY; ESTROUS-CYCLE; GESTATION; GROWTH; VASCULARITY; CATTLE; ARTERY; CONCEPTUS AB The objective was to examine the effect of maternal nutrient restriction followed by realimentation during midgestation on uterine blood flow (BF). On Day 30 of pregnancy, lactating, multiparous Simmental beef cows were assigned randomly to treatments: control (CON; 100% National Research Council; n = 6) and nutrient restriction (RES; 60% of CON; n = 4) from Day 30 to 140 (period 1), and thereafter, realimented to CON until Day 198 of gestation (period 2). Uterine BF, pulsatility index (PI), and resistance index (RI) were obtained from both the ipsilateral and contralateral uterine arteries via Doppler ultrasonography. Generalized least square analysis was performed. Ipsilateral uterine BF in both groups increased quadratically (P < 0.01) during period 1 and linearly (P < 0.01) during period 2. There was a treatment (P = 0.05) effect during period 2; where RES cows had greater ipsilateral BF versus CON. Ipsilateral uterine PI and RI decreased linearly (P <= 0.01) during period 1 across treatments. Contralateral uterine BF in CON cows tended (P < 0.09) to be greater versus RES in both periods. Contralateral PI in both groups increased linearly (P < 0.01) during period 1. Contralateral uterine RI was increased (P <= 0.05) in RES cows versus CON in both periods. There was no interaction or treatment effect (P >= 0.24) for total BF during either period. Nutrient restriction does not alter total uterine BF, but it may increase vascular resistance. However, up on realimentation, local conceptus-derived vasoactive factors appear to influence ipsilateral uterine BF. (C) 2014 Elsevier Inc. All rights reserved. C1 [Camacho, L. E.; Lemley, C. O.; Prezotto, L. D.; Bauer, M. L.; Swanson, K. C.; Vonnahme, K. A.] N Dakota State Univ, Dept Anim Sci, Fargo, ND 58105 USA. [Freetly, H. C.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Vonnahme, KA (reprint author), N Dakota State Univ, Dept Anim Sci, Fargo, ND 58105 USA. EM Kim.Vonnahme@ndsu.edu FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2009 to 65203 to 05812] FX The authors thank the employees of the NDSU Beef Cattle Research Complex and the Beef Research and Teaching Unit. The authors would also like to thank Jim Kirsch for his assistance with pregnancy detection, and David Buchanan for assistance with statistical analysis. Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA, and does not imply approval to the exclusion of other products that many be suitable. USDA is an equal opportunity provider and employer. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2009 to 65203 to 05812 from USDA National Institute of Food and Agriculture to KAV and KCS. NR 29 TC 6 Z9 6 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD JUN PY 2014 VL 81 IS 9 BP 1248 EP 1256 DI 10.1016/j.theriogenology.2014.02.006 PG 9 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA AG9AJ UT WOS:000335710600011 PM 24650930 ER PT J AU Alzaman, N Siegel, RD Schaefer, EJ AF Alzaman, Naweed Siegel, Richard D. Schaefer, Ernst John TI Prolonged Honeymoon Period in Type 1 Diabetic Patient with Low Carbohydrate Intake SO ENDOCRINE REVIEWS LA English DT Meeting Abstract C1 [Alzaman, Naweed] Tufts Med Ctr, Boston, MA USA. [Siegel, Richard D.] Tufts Med Ctr, Medford, MA USA. [Schaefer, Ernst John] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0163-769X EI 1945-7189 J9 ENDOCR REV JI Endocr. Rev. PD JUN PY 2014 VL 35 IS 3 SU S MA SUN-0974 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA V45GM UT WOS:000209805107042 ER PT J AU Anthanont, P Millar, JS Billheimer, J Cuchel, M Rader, DJ Schaefer, EJ AF Anthanont, Pimjai Millar, John S. Billheimer, Jeffrey Cuchel, Marina Rader, Daniel J. Schaefer, Ernst John TI A Novel Apolipoprotein A-I Truncation (ApoA-I-Mytilene) Associated with Decreased ApoA-I Production and Cholesterol Efflux SO ENDOCRINE REVIEWS LA English DT Meeting Abstract C1 [Anthanont, Pimjai; Schaefer, Ernst John] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. [Millar, John S.] Univ Penn, Sch Med, Perelman Sch Med, Philadelphia, PA 19104 USA. [Billheimer, Jeffrey; Cuchel, Marina; Rader, Daniel J.] Univ Penn, Sch Med, Philadelphia, PA 19104 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0163-769X EI 1945-7189 J9 ENDOCR REV JI Endocr. Rev. PD JUN PY 2014 VL 35 IS 3 SU S MA SUN-0868 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA V45GM UT WOS:000209805106046 ER PT J AU Jones, G Jones, D Teal, P Sapa, A Bill-Klula, I Wozniak, M Spokony, R Jadczak, S Pagel, J AF Jones, Grace Jones, Davy Teal, Peter Sapa, Agnes Bill-klula, Iwona Wozniak, Mietek Spokony, Rebecca Jadczak, Steve Pagel, John TI Participation of the Drosophila Homolog of ROR in RXR-Mediated Epidermal Morphogenesis SO ENDOCRINE REVIEWS LA English DT Meeting Abstract C1 [Jones, Grace; Jones, Davy; Jadczak, Steve; Pagel, John] Univ Kentucky, Lexington, KY USA. [Teal, Peter] USDA ARS, Gainesville, FL USA. [Sapa, Agnes; Bill-klula, Iwona; Wozniak, Mietek] Wroclaw Med Univ, Wroclaw, Poland. [Spokony, Rebecca] CUNY, Baruch Coll, New York, NY 10021 USA. NR 4 TC 0 Z9 0 U1 0 U2 0 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0163-769X EI 1945-7189 J9 ENDOCR REV JI Endocr. Rev. PD JUN PY 2014 VL 35 IS 3 SU S MA MON-0378 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA V45GM UT WOS:000209805100293 ER PT J AU Thorson, JF Prezotto, LD McCosh, RB Wright, EC Swanson, KC White, BR Freking, BA Oliver, WT Hileman, SM Lents, CA AF Thorson, Jennifer F. Prezotto, Ligia D. McCosh, Richard B. Wright, Elane C. Swanson, Kendall C. White, Brett R. Freking, Brad A. Oliver, William T. Hileman, Stanley M. Lents, Clay A. TI Effects of RFRP2 and RF9 on Secretion of Luteinizing Hormone in Prepubertal Gilts SO ENDOCRINE REVIEWS LA English DT Meeting Abstract C1 [Thorson, Jennifer F.; Wright, Elane C.; Freking, Brad A.; Oliver, William T.; Lents, Clay A.] ARS, USDA, USMARC, Clay Ctr, NE USA. [Prezotto, Ligia D.; Swanson, Kendall C.] North Dakota State Univ, Fargo, ND USA. [McCosh, Richard B.; Hileman, Stanley M.] West Virginia Univ, Morgantown, WV USA. [White, Brett R.] Univ Nebraska, Lincoln, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0163-769X EI 1945-7189 J9 ENDOCR REV JI Endocr. Rev. PD JUN PY 2014 VL 35 IS 3 SU S MA SUN-0669 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA V45GM UT WOS:000209805101301 ER PT J AU Braz, MG Braz, LG Braz, JR Salvadori, DM Yeum, KJ AF Braz, M. G. Braz, L. G. Braz, J. R. Salvadori, D. M. Yeum, K. -J TI The effects of anaesthesia maintained with isoflurane or propofol on antioxidative/oxidative biomarkers in patients undergoing surgery SO EUROPEAN JOURNAL OF ANAESTHESIOLOGY LA English DT Meeting Abstract C1 [Braz, M. G.; Braz, L. G.; Braz, J. R.] UNESP Univ Estadual Paulista, Dept Anaesthesiol, Fac Med Botucatu, Botucatu, SP, Brazil. [Salvadori, D. M.] UNESP Univ Estadual Paulista, Fac Med Botucatu, Pathol, Botucatu, SP, Brazil. [Yeum, K. -J] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0265-0215 EI 1365-2346 J9 EUR J ANAESTH JI Eur. J. Anaesth. PD JUN PY 2014 VL 31 SU 52 MA 1AP5-1 BP 16 EP 16 PG 1 WC Anesthesiology SC Anesthesiology GA V45QV UT WOS:000209832000046 ER PT J AU Thompson, D AF Thompson, Debbe TI Talk to Me, Please!: The Importance of Qualitative Research to Games for Health SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material ID FORMATIVE RESEARCH; ADOLESCENTS; NUTRITION C1 USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. RP Thompson, D (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM dit@bcm.edu NR 13 TC 7 Z9 7 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2014 VL 3 IS 3 BP 117 EP + DI 10.1089/g4h.2014.0023 PG 3 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CU2VX UT WOS:000363382800001 PM 26196170 ER PT J AU Crouch, JA AF Crouch, Jo Anne TI Colletotrichum caudatum s.l. is a species complex SO IMA FUNGUS LA English DT Article DE Andropogon gerardii; bioenergy; Colletotrichum ciliatum; Cymbopogon; Imperata cylindrica; Sorghastrum nutans; tallgrass prairie; Zoysia AB Colletotrichum caudatum sensu lato is a widespread fungal pathogen of warm-season grasses. The fungus is easily differentiated from other Colletotrichum species through the presence of a unique filiform appendage at the apex of the conidium. Multi-locus phylogenetic analysis of four DNA sequence markers from 21 isolates of C. caudatum s.l. from six grass hosts recovered the morphospecies as a well-supported monophyletic group. Although closely related to other Colletotrichum species pathogenic to warm-season grasses (e.g. C. sublineola, C. falcatum, C. navitas, C. graminicola), the sister taxon placement of C. caudatum remained unclear. Four major subgroups and three monotypic lineages were identified from the C. caudatum s.l. isolates. Despite the presence of localized, taxon-specific incongruence between gene trees and evidence for recombination in the dataset, application of genealogical concordance species recognition criteria diagnosed the four subgroups as phylogenetic species. Traditional morphology-based species concept defines C. caudatum as one species with a broad host range; however, multi-locus phylogenetic analyses refuted this model. Instead, isolates from different hosts were mainly segregated into different lineages. In particular, isolates from the type locale and host (USA, Sorghastrum nutans) collected within a 400 km radius were divided into three distinct species that corresponded with the three sampling sites. These data established that traditional morphological and ecological features are not informative for recognition of taxa within C. caudatum s.l., although there is some evidence that some species may be host specific. To stabilize the application of the name C. caudatum, DNA sequence data from the lectotype was generated, an epitype strain consistent with the type was designated and illustrated, and an emended description of C. caudatum sensu stricto is provided. Colletotrichum alcornii, C. baltimorense, C. somersetense, and C. zoysiae are described as new morphologically cryptic species related to C. caudatum s.s. C1 [Crouch, Jo Anne] USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave,B010A, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave,B010A, Beltsville, MD 20705 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090 NR 40 TC 8 Z9 10 U1 0 U2 3 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2014 VL 5 IS 1 BP 17 EP 30 PG 14 WC Mycology SC Mycology GA V46UJ UT WOS:000209908800017 PM 25083403 ER PT J AU Johnston, PR Seifert, KA Stone, JK Rossman, AY Marvanova, L AF Johnston, Peter R. Seifert, Keith A. Stone, Jeffrey K. Rossman, Amy Y. Marvanova, Ludmila TI Recommendations on generic names competing for use in Leotiomycetes (Ascomycota) SO IMA FUNGUS LA English DT Article DE Article 59; Ascomycetes; Fungi; inoperculate discomycetes; nomenclature; one fungus-one name; pleomorphic fungi; taxonomy; unit nomenclature AB In advancing to one scientific name for fungi, this paper treats genera competing for use in the phylogenetically defined class Leotiomycetes except for genera of Erysiphales. Two groups traditionally included in the so-called "inoperculate discomycetes" have been excluded from this class and are also not included here, specifically Geoglossomycetes and Orbiliomycetes. A recommendation is made about the generic name to use in cases in which two or more generic names are synonyms or taxonomically congruent along with the rationale for the recommendation. In some cases the recommended generic name does not have priority or is based on an asexual type species, thus needs to be protected and ultimately approved according to Art. 57.2 of the International Code of Nomenclature for algae, fungi and plants (ICN). A table is presented listing all competing generic names and their type species noting the recommended generic name. New combinations are introduced for the oldest epithet in the recommended genus including Ascocalyx berenice, Ascoconidium purpurascens, Ascocoryne albida, A. trichophora, Blumeriella filipendulae, B. ceanothi, Botrytis arachidis, B. fritillariae-pallidoflori, Calloria urticae, Calycellina aspera, Dematioscypha delicata, Dermea abietinum, D. boycei, D. stellata, Diplocarpon alpestre, D. fragariae, Godroniopsis peckii, Grovesinia moricola, Heterosphaera sublineolata, Hyphodiscus brachyconium, H. brevicollaris, H. luxurians, Leptotrochila campanulae, Monilinia polystroma, Neofabraea actinidae, N. citricarpa, N. vagabunda, Oculimacula aestiva, O. anguioides, Pezicula brunnea, P. californiae, P. cornina, P. diversispora, P. ericae, P. melanogena, P. querciphila, P. radicicola, P. rhizophila, Phialocephala piceae, Pilidium lythri, Rhabdocline laricis, Streptotinia streptothrix, Symphyosirinia parasitica, S. rosea, Unguiculariopsis caespitosa, and Vibrissea laxa. C1 [Johnston, Peter R.] Landcare Res, Private Bag 92170, Auckland 1142, New Zealand. [Seifert, Keith A.] Agr & Agri Food Canada, Biodivers Mycol & Microbiol, Ottawa, ON, Canada. [Stone, Jeffrey K.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Rossman, Amy Y.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Marvanova, Ludmila] Masaryk Univ, Inst Expt Biol, Czech Collect Microorganisms, Brno 62500, Czech Republic. RP Johnston, PR (reprint author), Landcare Res, Private Bag 92170, Auckland 1142, New Zealand. EM johnstonp@landcareresearch.co.nz FU Czech Collection of Microorganisms; Landcare Research Systematics Portfolio through Science and Innovation group of the New Zealand Ministry of Business, Innovation and Employment FX In obtaining input from the diverse user communities, the individuals listed below were consulted about their preferences for use of generic names. Although conflicting preferences could not be accommodated, all opinions and ideas were taken into consideration. These scientists include: Hans-Otto Baral, Christiane Baschien, Virginia Bianchinotti, Paul Cannon, Pedro Crous, Everett Hansen, David L. Hawksworth, Charles Hodges, Tsuyoshi Hosoya, Theresa Iturriaga, Paul M. Kirk, Richard Korf, Linda Kohn, Adrian Leuchtmann, Henrik Nilsson, Alan Phillips, Scott Redhead, Megan Romberg, Andrea Irene Romero, Conrad Schoch, Lynn Sigler, Matthew Smith, Wendy Untereiner, Gerard Verkley, Zheng Wang, Chang-Lin Xiao, and Wen-ying Zhuang. L. Marvanova acknowledges the financial support from the Czech Collection of Microorganisms. P.R. Johnston acknowledges financial support from the Landcare Research Systematics Portfolio through funding from the Science and Innovation group of the New Zealand Ministry of Business, Innovation and Employment. NR 167 TC 27 Z9 28 U1 0 U2 5 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2014 VL 5 IS 1 BP 91 EP 120 DI 10.5598/imafungus.2014.05.01.11 PG 30 WC Mycology SC Mycology GA V46UJ UT WOS:000209908800025 PM 25083411 ER PT J AU Quandt, CA Kepler, RM Gams, W Araujo, JPM Ban, S Evans, HC Hughes, D Humber, R Hywel-Jones, N Li, ZZ Luangsa-ard, JJ Rehner, SA Sanjuan, T Sato, H Shrestha, B Sung, GH Yao, YJ Zare, R Spatafora, JW AF Quandt, C. Alisha Kepler, Ryan M. Gams, Walter Araujo, Joao P. M. Ban, Sayaka Evans, Harry C. Hughes, David Humber, Richard Hywel-Jones, Nigel Li, Zengzhi Luangsa-ard, J. Jennifer Rehner, Stephen A. Sanjuan, Tatiana Sato, Hiroki Shrestha, Bhushan Sung, Gi-Ho Yao, Yi-Jian Zare, Rasoul Spatafora, Joseph W. TI Phylogenetic-based nomenclatural proposals for Ophiocordycipitaceae (Hypocreales) with new combinations in Tolypocladium SO IMA FUNGUS LA English DT Article DE arthropod-pathogens; Article 59; new combinations; nomenclature; Ophiocordycipitaceae; Tolypocladium AB Ophiocordycipitaceae is a diverse family comprising ecologically, economically, medicinally, and culturally important fungi. The family was recognized due to the polyphyly of the genus Cordyceps and the broad diversity of the mostly arthropod-pathogenic lineages of Hypocreales. The other two cordyceps-like families, Cordycipitaceae and Clavicipitaceae, will be revised taxonomically elsewhere. Historically, many species were placed in Cordyceps, but other genera have been described in this family as well, including several based on anamorphic features. Currently there are 24 generic names in use across both asexual and sexual life stages for species of Ophiocordycipitaceae. To reflect changes in Art. 59 in the International Code of Nomenclature for algae, fungi, and plants (ICN), we propose to protect and to suppress names within Ophiocordycipitaceae, and to present taxonomic revisions in the genus Tolypocladium, based on rigorous and extensively sampled molecular phylogenetic analyses. When approaching this task, we considered the principles of priority, monophyly, minimizing taxonomic revisions, and the practical utility of these fungi within the wider biological research community. C1 [Quandt, C. Alisha; Spatafora, Joseph W.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Kepler, Ryan M.; Rehner, Stephen A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Gams, Walter] Fungal Biodivers Ctr, CBS KNAW, Utrecht, Netherlands. [Araujo, Joao P. M.; Hughes, David] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Ban, Sayaka] Natl Inst Technol & Evaluat, Biol Resource Ctr, Chiba 2920818, Japan. [Evans, Harry C.] E UK Ctr, CAB Int, Egham TW20 9TY, Surrey, England. [Hughes, David] Penn State Univ, Dept Entomol, Ctr Infect Dis Dynam, State Coll, PA 16802 USA. [Humber, Richard] USDA ARS, Biol Integrated Pest Management Res, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Hywel-Jones, Nigel] Milton Biotech Ltd, Bangkok 10530, Thailand. [Li, Zengzhi] Anhui Agr Univ, Dept Forestry, Hefei 230036, Anhui, Peoples R China. [Luangsa-ard, J. Jennifer] Natl Ctr Genet Engn & Biotechnol BIOTEC, Microbe Interact Lab, Khlong Luang 12120, Pathum Thani, Thailand. [Sanjuan, Tatiana] Univ Antioquia, Fac Ciencias Nat, Inst Biol, Lab Taxon & Ecol Hongos, Medellin, Colombia. [Sanjuan, Tatiana] Univ Los Andes, Dept Ciencias Biol, Lab Micol & Fitopatol, Bogota, Colombia. [Sato, Hiroki] Forestry & Forest Prod Res Inst, Dept Forest Entomol, Tsukuba, Ibaraki 305, Japan. [Shrestha, Bhushan] Sungkyunkwan Univ, Inst Life Sci & Biotechnol, Suwon 440746, South Korea. [Sung, Gi-Ho] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Mushroom Res Div, Eumseong 369873, South Korea. [Yao, Yi-Jian] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China. [Zare, Rasoul] Iranian Res Inst Plant Protect, Dept Bot, Tehran 19395, Iran. RP Quandt, CA (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM owensbyc@science.oregonstate.edu FU NSF FX We would like to acknowledge the following individuals for their contribution to this manuscript: Priscila Chaverri, Mingjun Chen, Tsuyoshi Hosoya, Jae-Mo Sung, and James White. An NSF Graduate Research Fellowship supported C.A.Q. NR 79 TC 31 Z9 33 U1 0 U2 7 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2014 VL 5 IS 1 BP 121 EP 134 DI 10.5598/imafungus.2014.05.01.12 PG 14 WC Mycology SC Mycology GA V46UJ UT WOS:000209908800026 PM 25083412 ER PT J AU Qin, JW Chao, KL Kim, MS Lee, H Peng, YK AF Qin, Jianwei Chao, Kuanglin Kim, Moon S. Lee, Hoyoung Peng, Yankun TI Development of a Raman chemical imaging detection method for authenticating skim milk powder SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Raman imaging; Food safety; Milk; Adulterant; Image classification ID NEAR-INFRARED SPECTROSCOPY; NMR-SPECTROSCOPY; QUANTIFICATION; ADULTERANTS AB This research developed a Raman chemical imaging method for detecting multiple adulterants in skim milk powder. Ammonium sulfate, dicyandiamide, melamine, and urea were mixed into the milk powder as chemical adulterants in the concentration range of 0.1-5.0 %. A Raman imaging system with a 785-nm laser was used to acquire hyperspectral images in the wave-number range of 102-2,538 cm(-1) for a 25 x 25 mm(2) area of each mixture. A polynomial curve-fitting method was used to correct for the fluorescence background in the Raman images. An image classification method was developed based on single-band fluorescence-free images at unique Raman peaks of the adulterants. Raman chemical images were created to visualize identification and distribution of the multiple adulterant particles in the milk powder. A linear relationship was found between adulterant pixel number and adulterant concentration, demonstrating the potential of this Raman chemical imaging method for quantitative analysis of adulterants in the milk powder. C1 [Qin, Jianwei; Chao, Kuanglin; Kim, Moon S.; Lee, Hoyoung] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Peng, Yankun] China Agr Univ, Coll Engn, Beijing 100083, Peoples R China. RP Qin, JW (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Bldg 303,BARC East 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM jianwei.qin@ars.usda.gov NR 16 TC 8 Z9 8 U1 3 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD JUN PY 2014 VL 8 IS 2 BP 122 EP 131 DI 10.1007/s11694-014-9172-9 PG 10 WC Food Science & Technology SC Food Science & Technology GA CU6VN UT WOS:000363672900007 ER PT J AU Kandala, CV Sundaram, J AF Kandala, Chari V. Sundaram, Jaya TI Nondestructive moisture content determination of three different market type in-shell peanuts using near infrared reflectance spectroscopy SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE In-shell peanuts; Near infrared spectroscopy; Moisture content; Runners; Valencia; Virginia; Multiple linear regression AB A near infrared reflectance (NIR) method is presented here by which the average moisture content (MC) of about 100 g of in-shell peanuts could be determined rapidly and nondestructively. MCs of three market type peanuts, Runners, Valencia and Virginia were determined by this method while the peanuts were in their shells (in-shell peanuts). The MC range of the peanuts tested was between 6 and 26 %. NIR reflectance measurements were made at 1 nm intervals in the wavelength range of 1,000-1,800 nm and the spectral data was modeled using partial least squares regression analysis. Eight different models were developed by utilizing different data preprocessing methods such as, Norris-Gap first derivative with a gap size of 3, peak normalization with 1,680 nm (which is the no absorbance wavelength for water), and transformation from reflectance to absorption. Applying model fitness measures, a suitable model was selected out of these. Predicted values of the samples tested were compared with the values determined by the standard air-oven method. The predicted values agreed well with the air-oven values with an R-2 value better than 0.93 for all three types of in-shell peanuts. This method being rapid, nondestructive, and non contact, may be suitable for measuring and monitoring MCs of different types of peanuts, while they are in their shells itself, in the peanut industry. C1 [Kandala, Chari V.; Sundaram, Jaya] ARS, Natl Peanut Res Lab, USDA, Dawson, GA 39842 USA. RP Kandala, CV (reprint author), ARS, Natl Peanut Res Lab, USDA, Dawson, GA 39842 USA. EM chari.kandala@ars.usda.gov NR 13 TC 1 Z9 2 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD JUN PY 2014 VL 8 IS 2 BP 132 EP 141 DI 10.1007/s11694-014-9173-8 PG 10 WC Food Science & Technology SC Food Science & Technology GA CU6VN UT WOS:000363672900008 ER PT J AU Hall, WJ Schneider, M Thompson, D Volpe, SL Steckler, A Hall, JM Fisher, MR AF Hall, William J. Schneider, Margaret Thompson, Deborah Volpe, Stella L. Steckler, Allan Hall, John M. Fisher, M. Randall CA HLTH Study Grp TI School factors as barriers to and facilitators of a preventive intervention for pediatric type 2 diabetes SO TRANSLATIONAL BEHAVIORAL MEDICINE LA English DT Article DE School; Intervention; Prevention; Health behavior; Diabetes; Obesity ID PHYSICAL-EDUCATION INTERVENTION; HEALTH POLICIES; RATIONALE; NUTRITION; DESIGN; IMPLEMENTATION; COMPONENT; PROGRAMS; STUDENTS; TRIAL AB School-based interventions are essential to prevent pediatric obesity and type 2 diabetes. School environmental factors influence implementation of these interventions. This article examines how school factors acted as barriers to and facilitators of the HEALTHY intervention. The HEALTHY study was a cluster-randomized trial of a multicomponent intervention implemented in 21 schools. Interview data were analyzed to identify barriers and facilitators. Barriers included teacher frustration that intervention activities detracted from tested subjects, student resistance and misbehavior, classroom management problems, communication equipment problems, lack of teacher/staff engagement, high cost and limited availability of nutritious products, inadequate facility space, and large class sizes. Facilitators included teacher/staff engagement, effective classroom management, student engagement, schools with direct control over food service, support from school leaders, and adequate facilities and equipment. Contextual barriers and facilitators must be taken into account in the design and implementation of school-based health interventions. C1 [Hall, William J.; Steckler, Allan; Hall, John M.; Fisher, M. Randall] Univ N Carolina, Sch Nursing, Chapel Hill, NC 27599 USA. [Schneider, Margaret] Univ Calif Irvine, Dept Planning Policy & Design, Irvine, CA 92697 USA. [Thompson, Deborah] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Volpe, Stella L.] Drexel Univ, Dept Nutr Sci, Philadelphia, PA 19104 USA. RP Hall, WJ (reprint author), Univ N Carolina, Sch Nursing, Chapel Hill, NC 27599 USA. EM wjhall@unc.edu FU National Institute of Diabetes, Digestive, and Kidney Diseases of the National Institutes of Health [U01-DK61230, U01-DK61249, U01-DK61231, U01-DK61223]; American Diabetes Association FX This study was completed with funding from the National Institute of Diabetes, Digestive, and Kidney Diseases of the National Institutes of Health grant numbers U01-DK61230, U01-DK61249, U01-DK61231, and U01-DK61223, with additional support from the American Diabetes Association. In addition, an award from the Anne Linda Furstenburg Fund supported William J. Hall during data analysis and writing. NR 29 TC 2 Z9 2 U1 6 U2 10 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 1869-6716 EI 1613-9860 J9 TRANSL BEHAV MED JI Transl. Behav. Med. PD JUN PY 2014 VL 4 IS 2 BP 131 EP 140 DI 10.1007/s13142-013-0226-z PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CL2PL UT WOS:000356786400003 PM 24904696 ER PT J AU Karlen, DL Johnson, JMF AF Karlen, Douglas L. Johnson, Jane M. F. TI Crop Residue Considerations for Sustainable Bioenergy Feedstock Supplies SO BIOENERGY RESEARCH LA English DT Article DE Corn stover; Wheat straw; Soil organic carbon; Aggregation; Microbial community; Feedstock quality; Simulation modeling AB The anticipated 2014 launch of three full-scale corn stover bioenergy conversion facilities is a strong US market signal that cellulosic feedstock supplies must increase dramatically to supply the required biomass in a sustainable manner. This overview highlights research conducted by the USDA-Agricultural Research Service Renewable Energy Assessment Project (now known as the Resilient Economic Agricultural Practices) team as part of the National Institute for Food and Agriculture Sun Grant Regional Feedstock Partnership Corn Stover team. Stover and grain yield, soil organic carbon, soil aggregation, greenhouse gas, energy content of the stover, and several other factors affecting the fledgling bioenergy industry are addressed in this special issue of the journal. C1 [Karlen, Douglas L.] ARS, USDA, NLAE, Ames, IA 50011 USA. [Johnson, Jane M. F.] ARS, USDA, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Karlen, DL (reprint author), ARS, USDA, NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. EM Doug.Karlen@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 NR 12 TC 14 Z9 14 U1 4 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 465 EP 467 DI 10.1007/s12155-014-9407-y PG 3 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800001 ER PT J AU Benjamin, JG Karlen, DL AF Benjamin, Joseph G. Karlen, Douglas L. TI LLWR Techniques for Quantifying Potential Soil Compaction Consequences of Crop Residue Removal SO BIOENERGY RESEARCH LA English DT Article DE Least limiting water range; LLWR; Crop residue removal; Soil quality; Soil physical properties ID LIMITING WATER RANGE; ORGANIC-CARBON; BULK-DENSITY; RETENTION CHARACTERISTICS; BIOETHANOL PRODUCTION; TILLAGE SYSTEMS; CORN STOVER; MATTER; PREDICTION; MANAGEMENT AB Harvesting crop residues for bioenergy or bio-product production may decrease soil organic matter (SOM) content, resulting in the degradation of soil physical properties and ultimately soil productivity. Using the least limiting water range (LLWR) to evaluate improvement or degradation of soil physical properties in response to SOM changes has generally been hampered by the extensive amount of data needed to parameterize limiting factor models for crop production. Our objective was to evaluate five pedotransfer functions to determine their effectiveness in predicting soil water holding capacity in response to different SOM levels. Similarly, two other pedotransfer functions were evaluated to determine the effects of SOM on cone index values. Predictions of field capacity and wilting point water content as well as the cone index-water content-bulk density relationship of soil strength using the pedotransfer functions were compared with field data from two tillage experiments near Akron, CO that had a range of SOM concentrations. Equations previously developed by da Silva and Kay gave the best estimates of LLWR for the pedotransfer functions we evaluated. These equations were then used to illustrate LLWR changes in response to different soil and crop management practices on a Duroc loam near Sidney, NE. The results showed that tillage and, possibly, soil erosion decreased the LLWR as tillage intensity increased. Therefore, we recommend that crop residue removal rates be limited to rates that maintain or increase SOM content to ensure soil physical conditions are not degraded. C1 [Benjamin, Joseph G.] ARS, Cent Great Plains Res Stn, USDA, Akron, CO 80720 USA. [Karlen, Douglas L.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Benjamin, JG (reprint author), ARS, Cent Great Plains Res Stn, USDA, 40335 Cty Rd GG, Akron, CO 80720 USA. EM Joseph.Benjamin@ars.usda.gov FU USDA-Agricultural Research Service as part of the USDA-ARS-Resilient Economic Agricultural Practices project; North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041] FX This study was funded by the USDA-Agricultural Research Service as part of the USDA-ARS-Resilient Economic Agricultural Practices project with additional funds from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy-Office of Biomass Programs under award number DE-FC36-05GO85041. NR 38 TC 7 Z9 7 U1 6 U2 45 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 468 EP 480 DI 10.1007/s12155-013-9400-x PG 13 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800002 ER PT J AU Johnson, JMF Novak, JM Varvel, GE Stott, DE Osborne, SL Karlen, DL Lamb, JA Baker, J Adler, PR AF Johnson, Jane M. F. Novak, Jeff M. Varvel, Gary E. Stott, Diane E. Osborne, Shannon L. Karlen, Douglas L. Lamb, John A. Baker, John Adler, Paul R. TI Crop Residue Mass Needed to Maintain Soil Organic Carbon Levels: Can It Be Determined? SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Second generation feedstock; Sustainable; Renewable energy ID CORN STOVER FEEDSTOCK; CONSERVATION TILLAGE; REMOVAL IMPACT; UNITED-STATES; MATTER; MODEL; BIOENERGY; ROTATION; CQESTR; SEQUESTRATION AB Corn's (Zea mays L.) stover is a potential nonfood, herbaceous bioenergy feedstock. A vital aspect of utilizing stover for bioenergy production is to establish sustainable harvest criteria that avoid exacerbating soil erosion or degrading soil organic carbon (SOC) levels. Our goal is to empirically estimate the minimum residue return rate required to sustain SOC levels at numerous locations and to identify which macroscale factors affect empirical estimates. Minimum residue return rate is conceptually useful, but only if the study is of long enough duration and a relationship between the rate of residue returned and the change in SOC can be measured. About one third of the Corn Stover Regional Partnership team (Team) sites met these criteria with a minimum residue return rate of 3.9 +/- 2.18 Mg stover ha(-1) yr(-1), n = 6. Based on the Team and published corn-based data (n = 35), minimum residue return rate was 6.38 +/- 2.19 Mg stover ha(-1) yr(-1), while including data from other cropping systems (n = 49), the rate averaged 5.74 +/- 2.36 Mg residue ha(-1) yr(-1). In broad general terms, keeping about 6 Mg residue ha(-1) yr(-1) maybe a useful generic rate as a point of discussion; however, these analyses refute that a generic rate represents a universal target on which to base harvest recommendations at a given site. Empirical data are needed to calibrate, validate, and refine process-based models so that valid sustainable harvest rate guidelines are provided to producers, industry, and action agencies. C1 [Johnson, Jane M. F.] ARS, N Cent Soil Conservat Res Lab, USDA, Morris, MN 56267 USA. [Novak, Jeff M.] ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, Florence, SC 29501 USA. [Varvel, Gary E.] ARS, Agroecosyst Management Res Unit, USDA, Lincoln, NE 68583 USA. [Stott, Diane E.] ARS, Natl Soil Eros Res Lab, USDA, W Lafayette, IN 47907 USA. [Osborne, Shannon L.] ARS, North Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. [Karlen, Douglas L.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. [Lamb, John A.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Baker, John] Univ Minnesota, ARS, Soil & Water Management Res Unit, USDA, St Paul, MN 55108 USA. [Adler, Paul R.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. RP Johnson, JMF (reprint author), ARS, N Cent Soil Conservat Res Lab, USDA, 803 Iowa Ave, Morris, MN 56267 USA. EM Jane.Johnson@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU US Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS Renewable Energy Assessment Project (REAP); North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041] FX Funding for this project was provided by the US Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS Renewable Energy Assessment Project (REAP). Additional funding was provided by the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy-Office of Biomass Programs under award no. DE-FC36-05GO85041 NR 46 TC 21 Z9 21 U1 3 U2 53 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 481 EP 490 DI 10.1007/s12155-013-9402-8 PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800003 ER PT J AU Campbell, EE Johnson, JMF Jin, VL Lehman, RM Osborne, SL Varvel, GE Paustian, K AF Campbell, Eleanor E. Johnson, Jane M. F. Jin, Virginia L. Lehman, R. Michael Osborne, Shannon L. Varvel, Gary E. Paustian, Keith TI Assessing the Soil Carbon, Biomass Production, and Nitrous Oxide Emission Impact of Corn Stover Management for Bioenergy Feedstock Production Using DAYCENT SO BIOENERGY RESEARCH LA English DT Article DE DAYCENT; Soil carbon change; Corn stover; Bioenergy; N2O emissions ID RESIDUE REMOVAL IMPACT; CROP RESIDUE; ORGANIC-CARBON; TILLAGE; SYSTEMS; MODEL; DYNAMICS; MAIZE; FERTILIZATION; QUALITY AB Harvesting crop residue needs to be managed to protect agroecosystem health and productivity. DAYCENT, a process-based modeling tool, may be suited to accommodate region-specific factors and provide regional predictions for a broad array of agroecosystem impacts associated with corn stover harvest. Grain yield, soil C, and N2O emission data collected at Corn Stover Regional Partnership experimental sites were used to test DAYCENT performance modeling the impacts of corn stover removal. DAYCENT estimations of stover yields were correlated and reasonably accurate (adjusted r (2) = 0.53, slope = 1.18, p << 0.001, intercept = 0.36, p = 0.11). Measured and simulated average grain yields across sites did not differ as a function of residue removal, but the model tended to underestimate average measured grain yields. Modeled and measured soil organic carbon (SOC) change for all sites were correlated (adjusted r (2) = 0.54, p << 0.001), but DAYCENT overestimated SOC loss with conventional tillage. Simulated and measured SOC change did not vary by residue removal rate. DAYCENT simulated annual N2O flux more accurately at low rates (a parts per thousand currency sign2-kg N2O-N ha(-1) year(-1)) but underestimated when emission rates were > 3-kg N2O-N ha(-1) year(-1). Overall, DAYCENT performed well at simulating stover yields and low N2O emission rates, reasonably well when simulating the effects of management practices on average grain yields and SOC change, and poorly when estimating high N2O emissions. These biases should be considered when DAYCENT is used as a decision support tool for recommending sustainable corn stover removal practices to advance bioenergy industry based on corn stover feedstock material. C1 [Campbell, Eleanor E.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Johnson, Jane M. F.] USDA ARS, Morris, MN 56267 USA. [Jin, Virginia L.; Varvel, Gary E.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Lehman, R. Michael; Osborne, Shannon L.] USDA ARS, Brookings, SD 57006 USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. RP Campbell, EE (reprint author), Colorado State Univ, Nat Resource Ecol Lab, 1231 East Dr, Ft Collins, CO 80523 USA. EM nell.campbell@colostate.edu RI Paustian, Keith/L-7593-2016; OI Lehman, Michael/0000-0002-3391-3178; Johnson, Jane/0000-0002-1687-4007 FU US Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Renewable Energy Assessment Project (REAP); North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041]; US Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041] FX Funding for this project was provided by the US Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Renewable Energy Assessment Project (REAP), renamed USDA-ARS-Resilient Economic Agricultural Practices (REAP). Additional funding was provided by the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy-Office of Biomass Programs under award number DE-FC36-05GO85041. Many thanks is given to Dr. William Parton, Steve Williams, and Jonathan Fields for their assistance with developing this manuscript. NR 52 TC 9 Z9 9 U1 2 U2 44 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 491 EP 502 DI 10.1007/s12155-014-9414-z PG 12 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800004 ER PT J AU Baker, JM Fassbinder, J Lamb, JA AF Baker, John M. Fassbinder, Joel Lamb, John A. TI The Impact of Corn Stover Removal on N2O Emission and Soil Respiration: an Investigation with Automated Chambers SO BIOENERGY RESEARCH LA English DT Article DE Stover removal; Greenhouse gases; N2O; Respiration ID NITROUS-OXIDE EMISSIONS; CROP RESIDUES; CARBON; QUALITY; TILLAGE; SYSTEMS; FIELD; FLUX AB Corn stover removal, whether for silage, bedding, or bioenergy production, could have a variety of environmental consequences through its effect on soil processes, particularly N2O production and soil respiration. Because these effects may be episodic in nature, weekly snapshots with static chambers may not provide a complete picture. We adapted commercially available automated soil respiration chambers by incorporating a portable N2O analyzer, allowing us to measure both CO2 and N2O fluxes on an hourly basis through two growing seasons in a corn field in southern Minnesota, from spring 2010 to spring 2012. This site was part of a USDA multilocation research project for five growing seasons, 2008-2012, with three levels of stover removal: zero, full, and intermediate. Initially in spring 2010, two chambers were placed in each of the treatments, but following planting in 2011, the configuration was changed, with four chambers installed on zero removal plots and four on full removal plots. The cumulative data revealed no significant difference in N2O emission as a function of stover removal. CO2 loss from the full removal plots was slightly lower than that from the zero removal plots, but the difference between treatments was much smaller than the amount of C removed in the residue, implying loss of soil carbon from the full removal plots. This is consistent with soil sampling data, which showed that in five of six sampled blocks, the SOC change in the full removal treatments was negative relative to the zero removal plots. We conclude that (a) full stover removal may have little impact on N2O production, and (b) while it will reduce soil CO2 production, the reduction will not be commensurate with the decrease in fresh carbon inputs and, thus, will result in SOC loss. C1 [Baker, John M.; Fassbinder, Joel] USDA ARS, St Paul, MN 55108 USA. [Lamb, John A.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Baker, JM (reprint author), USDA ARS, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM john.baker@ars.usda.gov FU USDA-Agricultural Research Service (ARS) as part of the USDA-ARS-REAP (Resilient Economic Agricultural Practices) project; North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy (DOE)-Bioenergy Technology Office (BETO) [DE-FC36-05GO85041] FX This research was funded by the USDA-Agricultural Research Service (ARS) as part of the USDA-ARS-REAP (Resilient Economic Agricultural Practices) project, with additional funding provided by the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy (DOE)-Office of Biomass Programs [now Bioenergy Technology Office (BETO)] under award number DE-FC36-05GO85041. NR 21 TC 7 Z9 7 U1 2 U2 46 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 503 EP 508 DI 10.1007/s12155-014-9412-1 PG 6 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800005 ER PT J AU Birrell, SJ Karlen, DL Wirt, A AF Birrell, Stuart J. Karlen, Douglas L. Wirt, Adam TI Development of Sustainable Corn Stover Harvest Strategies for Cellulosic Ethanol Production SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Sustainable feedstock production; Nutrient removal; Soil organic carbon ID SOIL QUALITY; CARBON AB To prepare for a 2014 launch of commercial scale cellulosic ethanol production from corn/maize (Zea mays L.) stover, POET-DSM near Emmetsburg, IA has been working with farmers, researchers, and equipment dealers through "Project Liberty" on harvest, transportation, and storage logistics of corn stover for the past several years. Our objective was to evaluate seven stover harvest strategies within a 50-ha (125 acres) site on very deep, moderately well to poorly drained Mollisols, developed in calcareous glacial till. The treatments included the following: conventional grain harvest (no stover harvest), grain plus a second-pass rake and bale stover harvest, and single-pass grain plus cob-only biomass, grain plus vegetative material other than grain [(MOG) consisting of cobs, husks, and upper plant parts], grain plus all vegetative material from the ear shank upward (high cut), and all vegetative material above a 10 cm stubble height (low cut), with a John Deere 9750 STS combine, and grain plus direct baling of MOG with an AgCo harvesting system. Average grain yields were 11.4, 10.1, 9.7, and 9.5 Mg ha(-1) for 2008, 2009, 2010, and 2011, respectively. Average stover harvest ranged from 0 to 5.6 Mg ha(-1) and increased N, P, and K removal by an average of 11, 1.6, and 15 kg Mg-1, respectively. Grain yield in 2009 showed a significant positive response to higher 2008 stover removal rates, but grain yield was not increased in 2010 or 2011 due to prior-year stover harvest. High field losses caused the direct-bale treatment to have significantly lower grain yield in 2011 because the AgCo system could not pick up the severely lodged crop. We conclude that decreases in grain yield across the 4 years were due more to seasonal weather patterns, spatial variability, and not rotating crops than to stover harvest. C1 [Birrell, Stuart J.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Wirt, Adam] POET DSM, Sioux Falls, SD 57104 USA. RP Karlen, DL (reprint author), ARS, USDA, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM sbirrell@iastate.edu; Doug.Karlen@ars.usda.gov FU POET-DSM Research; USDA-ARS Resilient Economic Agricultural Practices (REAP) project; North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy (DOE)-Bioenergy Technology Office (BETO) [DE-FC36-05GO85041] FX The US Department of Agriculture offers its programs to all eligible persons regardless of race, color, age, sex, or national origin, and is an equal opportunity employer. This research was funded by POET-DSM Research, the USDA-ARS Resilient Economic Agricultural Practices (REAP) project with additional funds from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy (DOE)-Office of Biomass Programs [now known as the Bioenergy Technology Office (BETO)] under award number DE-FC36-05GO85041. NR 18 TC 8 Z9 8 U1 4 U2 40 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 509 EP 516 DI 10.1007/s12155-014-9418-8 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800006 ER PT J AU Jin, VL Baker, JM Johnson, JMF Karlen, DL Lehman, RM Osborne, SL Sauer, TJ Stott, DE Varvel, GE Venterea, RT Schmer, MR Wienhold, BJ AF Jin, Virginia L. Baker, John M. Johnson, Jane M. -F. Karlen, Douglas L. Lehman, R. Michael Osborne, Shannon L. Sauer, Thomas J. Stott, Diane E. Varvel, Gary E. Venterea, Rodney T. Schmer, Marty R. Wienhold, Brian J. TI Soil Greenhouse Gas Emissions in Response to Corn Stover Removal and Tillage Management Across the US Corn Belt SO BIOENERGY RESEARCH LA English DT Article DE Corn stover; Tillage; Residue management; Static vented chamber; Carbon dioxide; Nitrous oxide; Methane ID NITROUS-OXIDE EMISSIONS; CARBON SEQUESTRATION; LONG-TERM; RESIDUE MANAGEMENT; ORGANIC-CARBON; N2O EMISSIONS; CONVENTIONAL TILLAGE; BIOFUEL PRODUCTION; CROPPING SYSTEMS; REDUCED TILLAGE AB In-field measurements of direct soil greenhouse gas (GHG) emissions provide critical data for quantifying the net energy efficiency and economic feasibility of crop residue-based bioenergy production systems. A major challenge to such assessments has been the paucity of field studies addressing the effects of crop residue removal and associated best practices for soil management (i.e., conservation tillage) on soil emissions of carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4). This regional survey summarizes soil GHG emissions from nine maize production systems evaluating different levels of corn stover removal under conventional or conservation tillage management across the US Corn Belt. Cumulative growing season soil emissions of CO2, N2O, and/or CH4 were measured for 2-5 years (2008-2012) at these various sites using a standardized static vented chamber technique as part of the USDA-ARS's Resilient Economic Agricultural Practices (REAP) regional partnership. Cumulative soil GHG emissions during the growing season varied widely across sites, by management, and by year. Overall, corn stover removal decreased soil total CO2 and N2O emissions by -4 and -7 %, respectively, relative to no removal. No management treatments affected soil CH4 fluxes. When aggregated to total GHG emissions (Mg CO2 eq ha(-1)) across all sites and years, corn stover removal decreased growing season soil emissions by -5 +/- 1 % (mean +/- se) and ranged from -36 % to 54 % (n = 50). Lower GHG emissions in stover removal treatments were attributed to decreased C and N inputs into soils, as well as possible microclimatic differences associated with changes in soil cover. High levels of spatial and temporal variabilities in direct GHG emissions highlighted the importance of site-specific management and environmental conditions on the dynamics of GHG emissions from agricultural soils. C1 [Jin, Virginia L.; Varvel, Gary E.; Schmer, Marty R.; Wienhold, Brian J.] Univ Nebraska, ARS, USDA, Lincoln, NE 68583 USA. [Baker, John M.; Venterea, Rodney T.] ARS, USDA, St Paul, MN 55108 USA. [Johnson, Jane M. -F.] ARS, USDA, Morris, MN 56267 USA. [Karlen, Douglas L.; Sauer, Thomas J.] ARS, USDA, Ames, IA 50011 USA. [Lehman, R. Michael; Osborne, Shannon L.] ARS, USDA, Brookings, SD 57006 USA. [Stott, Diane E.] ARS, USDA, W Lafayette, IN 47907 USA. RP Jin, VL (reprint author), Univ Nebraska, ARS, USDA, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM Virginia.Jin@ars.usda.gov RI Venterea, Rodney/A-3930-2009; OI Lehman, Michael/0000-0002-3391-3178; Johnson, Jane/0000-0002-1687-4007 FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Renewable Energy Assessment Project (REAP); North Central Regional SunGrant Center at South Dakota State University through United States Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041] FX Funding for this project was provide by the United States Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Renewable Energy Assessment Project (REAP), renamed USDA-ARS-Resilient Economic Agricultural Practices (REAP). Additional funding was from the North Central Regional SunGrant Center at South Dakota State University through a grant provided by the United States Department of Energy-Office of Biomass Programs under award number DE-FC36-05GO85041. Technical assistance in the field and/or laboratory is acknowledged from Kayla Miller, Meggan Kowalski, Kurt Dagel, Amy Christie, Sharon Nichols, Don Watts, Joe Million, Jerry Martin, Ray Winans, Anthony Shriner, Warren Busscher, Carla Ahlschwede, Chris Bauer, Aaron Bereuter, Todd Boman, Robert Harrington, Molly Hoffbauer, Angel Iverson, Tanner Lockhorn, Paul Koerner, Nate Mellor, Dan Miller, Sara Murray, Marty Schmer, Susan Siragusa-Ortman, Steve Swanson, Tyler Goeschel, David Walla, Kevin Jensen, Dawn Schroeder, Gary Radke, Tim Parkin, Amy Morrow, and Bryan Woodbury. Special thanks to Nancy Barbour, Jon Starr, and GregWilson for the assistance with the REAP database. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 60 TC 11 Z9 11 U1 2 U2 85 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 517 EP 527 DI 10.1007/s12155-014-9421-0 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800007 ER PT J AU Karlen, DL Birrell, SJ Johnson, JMF Osborne, SL Schumacher, TE Varvel, GE Ferguson, RB Novak, JM Fredrick, JR Baker, JM Lamb, JA Adler, PR Roth, GW Nafziger, ED AF Karlen, Douglas L. Birrell, Stuart J. Johnson, Jane M. F. Osborne, Shannon L. Schumacher, Thomas E. Varvel, Gary E. Ferguson, Richard B. Novak, Jeff M. Fredrick, James R. Baker, John M. Lamb, John A. Adler, Paul R. Roth, Greg W. Nafziger, Emerson D. TI Multilocation Corn Stover Harvest Effects on Crop Yields and Nutrient Removal SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Sustainable feedstock production; Nutrient removal ID RESIDUE REMOVAL; QUALITY AB Corn (Zea mays L.) stover was identified as an important feedstock for cellulosic bioenergy production because of the extensive area upon which the crop is already grown. This report summarizes 239 site-years of field research examining effects of zero, moderate, and high stover removal rates at 36 sites in seven different states. Grain and stover yields from all sites as well as N, P, and K removal from 28 sites are summarized for nine longitude and six latitude bands, two tillage practices (conventional vs no tillage), two stover-harvest methods (machine vs calculated), and two crop rotations {continuous corn (maize) vs corn/soybean [Glycine max (L.) Merr.]}. Mean grain yields ranged from 5.0 to 12.0 Mg ha(-1) (80 to 192 bu ac(-1)). Harvesting an average of 3.9 or 7.2 Mg ha(-1) (1.7 or 3.2 tons ac(-1)) of the corn stover resulted in a slight increase in grain yield at 57 and 51 % of the sites, respectively. Average no-till grain yields were significantly lower than with conventional tillage when stover was not harvested, but not when it was collected. Plant samples collected between physiological maturity and combine harvest showed that compared to not harvesting stover, N, P, and K removal was increased by 24, 2.7, and 31 kg ha(-1), respectively, with moderate (3.9 Mg ha(-1)) harvest and by 47, 5.5, and 62 kg ha(-1), respectively, with high (7.2 Mg ha(-1)) removal. This data will be useful for verifying simulation models and available corn stover feedstock projections, but is too variable for planning site-specific stover harvest. C1 [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Birrell, Stuart J.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Johnson, Jane M. F.] ARS, USDA, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Osborne, Shannon L.] ARS, USDA, NCARL, Brookings, SD 57006 USA. [Schumacher, Thomas E.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57006 USA. [Varvel, Gary E.] ARS, USDA, AMRU, Lincoln, NE 68583 USA. [Ferguson, Richard B.] Univ Nebraska, Dept Agron, Lincoln, NE 68583 USA. [Novak, Jeff M.] ARS, USDA, CPSWPRC, Florence, SC 29501 USA. [Fredrick, James R.] Clemson Univ, PDREC, Florence, SC 29501 USA. [Baker, John M.] ARS, USDA, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Lamb, John A.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Adler, Paul R.] ARS, USDA, PSWMRU, University Pk, PA 16802 USA. [Roth, Greg W.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Nafziger, Emerson D.] Univ Illinois, Dept Crop Sci, Champaign, IL 61820 USA. RP Karlen, DL (reprint author), ARS, USDA, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM Doug.Karlen@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU USDA-Agricultural Research Service as part of the USDA-ARS Resilient Economic Agricultural Practices (REAP); North Central Regional Sun Grant Center at South Dakota State University through U.S. Department of Energy (DOE)-Bioenergy Technology Office (BETO) [DE-FC36-05GO85041] FX The U.S. Department of Agriculture offers its programs to all eligible persons regardless of race, color, age, sex, or national origin and is an equal-opportunity employer. This research was funded by the USDA-Agricultural Research Service as part of the USDA-ARS Resilient Economic Agricultural Practices (REAP) project with additional funds from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the U.S. Department of Energy (DOE)-Office of Biomass Programs [now known as the Bioenergy Technology Office (BETO)] under award number DE-FC36-05GO85041. NR 24 TC 16 Z9 16 U1 2 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 528 EP 539 DI 10.1007/s12155-014-9419-7 PG 12 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800008 ER PT J AU Lehman, RM Ducey, TF Jin, VL Acosta-Martinez, V Ahlschwede, CM Jeske, ES Drijber, RA Cantrell, KB Frederick, JR Fink, DM Osborne, SL Novak, JM Johnson, JMF Varvel, GE AF Lehman, R. Michael Ducey, Thomas F. Jin, Virginia L. Acosta-Martinez, Veronica Ahlschwede, Carla M. Jeske, Elizabeth S. Drijber, Rhae A. Cantrell, Keri B. Frederick, James R. Fink, Darci M. Osborne, Shannon L. Novak, Jeff M. Johnson, Jane M. F. Varvel, Gary E. TI Soil Microbial Community Response to Corn Stover Harvesting Under Rain-Fed, No-Till Conditions at Multiple US Locations SO BIOENERGY RESEARCH LA English DT Article DE Corn stover; Maize; Microbial community; Residue management; Soil microorganisms; Soil quality ID ORGANIC-MATTER FRACTIONS; RESIDUE REMOVAL IMPACT; PARTICLE-SIZE; NITROGEN MINERALIZATION; CARBON SEQUESTRATION; ELEVATED CO2; BACTERIAL; BIOMASS; AGROECOSYSTEMS; DECOMPOSITION AB Harvesting of corn stover (plant residues) for cellulosic ethanol production must be balanced with the requirement for returning plant residues to agricultural fields to maintain soil structure, fertility, crop protection, and other ecosystem services. High rates of corn stover removal can be associated with decreased soil organic matter (SOM) quantity and quality and increased highly erodible soil aggregate fractions. Limited data are available on the impact of stover harvesting on soil microbial communities which are critical because of their fundamental relationships with C and N cycles, soil fertility, crop protection, and stresses that might be imposed by climate change. Using fatty acid and DNA analyses, we evaluated relative changes in soil fungal and bacterial densities and fungal-to-bacterial (F:B) ratios in response to corn stover removal under no-till, rain-fed management. These studies were performed at four different US locations with contrasting soil-climatic conditions. At one location, residue removal significantly decreased F:B ratios. At this location, cover cropping significantly increased F:B ratios at the highest level of residue removal and thus may be an important practice to minimize changes in soil microbial communities where corn stover is harvested. We also found that in these no-till systems, the 0- to 5-cm depth interval is most likely to experience changes, and detectable effects of stover removal on soil microbial community structure will depend on the duration of stover removal, sampling time, soil type, and annual weather patterns. No-till practices may have limited the rate of change in soil properties associated with stover removal compared to more extensive changes reported at a limited number of tilled sites. Documenting changes in soil microbial communities with stover removal under differing soil-climatic and management conditions will guide threshold levels of stover removal and identify practices (e.g., no-till, cover cropping) that may mitigate undesirable changes in soil properties. C1 [Lehman, R. Michael; Fink, Darci M.; Osborne, Shannon L.] ARS, North Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. [Ducey, Thomas F.; Cantrell, Keri B.; Novak, Jeff M.] ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, Florence, SC USA. [Jin, Virginia L.; Ahlschwede, Carla M.; Varvel, Gary E.] ARS, USDA, Lincoln, NE USA. [Acosta-Martinez, Veronica] ARS, Cropping Syst Res Lab, USDA, Lubbock, TX USA. [Ahlschwede, Carla M.; Jeske, Elizabeth S.; Drijber, Rhae A.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Frederick, James R.] Clemson Univ, Dept Entomol Soils & Plant Sci, Clemson, SC USA. [Johnson, Jane M. F.] ARS, N Cent Soil Conservat Res Lab, USDA, Morris, MN USA. RP Lehman, RM (reprint author), ARS, North Cent Agr Res Lab, USDA, 2923 Medary Ave, Brookings, SD 57006 USA. EM michael.lehman@ars.usda.gov OI Ducey, Thomas/0000-0001-8199-0584; Lehman, Michael/0000-0002-3391-3178; Johnson, Jane/0000-0002-1687-4007 FU USDA-Agricultural Research Service (ARS) as part of the USDA-ARS-REAP (Resilient Economic Agricultural Practices) project; North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy (DOE)-Bioenergy Technology Office (BETO) [DE-FC36-05GO85041] FX This research was funded by the USDA-Agricultural Research Service (ARS) as part of the USDA-ARS-REAP (Resilient Economic Agricultural Practices) project, with additional funding provided by the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy (DOE)-Office of Biomass Programs [now Bioenergy Technology Office (BETO)] under award number DE-FC36-05GO85041. Technical assistance in the field and/or lab is acknowledged from Kayla Miller, Meggan Kowalski, Kurt Dagel, Amy Christie, Don Watts, Joe Million, Jerry Martin, Ray Winans, Anthony Shriner, Warren Busscher, Chris Bauer, Aaron Bereuter, Dennis Francis, Paul Koerner, Susan Siragusa-Ortman, Jamie Pesek, Steve Swanson, David Walla, Chad Rollofson, Gary Amundson, and Nancy Barbour. NR 51 TC 8 Z9 8 U1 7 U2 78 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 540 EP 550 DI 10.1007/s12155-014-9417-9 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800009 ER PT J AU Mourtzinis, S Cantrell, KB Arriaga, FJ Balkcom, KS Novak, JM Frederick, JR Karlen, DL AF Mourtzinis, Spyridon Cantrell, Keri B. Arriaga, Francisco J. Balkcom, Kipling S. Novak, Jeff M. Frederick, James R. Karlen, Douglas L. TI Distribution of Structural Carbohydrates in Corn Plants Across the Southeastern USA SO BIOENERGY RESEARCH LA English DT Article DE Corn; Carbohydrates; Lignin; Cellulose; Distribution; Rye; Residue removal ID BIOETHANOL PRODUCTION; STOVER COMPOSITION; COVER CROPS; DEGRADATION; HYDROLYSIS; FEEDSTOCK; FRACTIONS; ETHANOL; YIELD; NIRS AB Quantifying lignin and carbohydrate composition of corn (Zea mays L.) is important to support the emerging cellulosic biofuels industry. Therefore, field studies with 0 or 100 % stover removal were established in Alabama and South Carolina as part of the Sun Grant Regional Partnership Corn Stover Project. In Alabama, cereal rye (Secale cereale L.) was also included as an additional experimental factor, serving as a winter cover crop. Plots were located on major soil types representative of their respective states: Compass and Decatur soils in Alabama and a Coxville/Rains-Goldsboro-Lynchburg soil association in South Carolina. Lignin and structural carbohydrate concentrations in the whole (above-ground) plant, cobs, vegetation excluding cobs above the primary ear (top), vegetation below the primary ear (bottom), and vegetation from above the primary ear including cobs (above-ear fraction) were determined using near-infrared spectroscopy (NIRS). The distribution of lignin, ash, and structural carbohydrates varied among plant fractions, but neither inclusion of a rye cover crop nor the stover harvest treatments consistently affected carbohydrate concentrations within locations. Total precipitation and average air temperature during the growing season were strongly correlated with stover composition indicating that weather conditions may have multiple effects on potential biofuel production (i.e., not only yield but also stover quality). When compared to the above-ear fractions, bottom plant partitions contained greater lignin concentrations. Holocellulose concentration was consistently greater in the above-ear fractions at all three locations. Data from this study suggests that the above-ear plant portions have the most desirable characteristics for cellulosic ethanol production via fermentation in the southeastern USA. C1 [Cantrell, Keri B.; Novak, Jeff M.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Arriaga, Francisco J.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Balkcom, Kipling S.] ARS, Natl Soil Dynam Res Lab, USDA, Auburn, AL 36832 USA. [Frederick, James R.] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29501 USA. [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM szm0020@tigermail.auburn.edu FU Alabama Wheat and Feed Grain Producers Commission of the Alabama Farmers Federation; North Central Regional Sun Grant Center at South Dakota State University through USDOE-Office of Biomass Programs [DE-FC36-05GO85041] FX Partial funding for this research was provided by the Alabama Wheat and Feed Grain Producers Commission of the Alabama Farmers Federation. This study is a contribution of the multilocation USDA-ARS-REAP, supported in part by supplemental funding from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the USDOE-Office of Biomass Programs under award number DE-FC36-05GO85041. NR 31 TC 6 Z9 6 U1 0 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 551 EP 558 DI 10.1007/s12155-014-9429-5 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800010 ER PT J AU Osborne, SL Johnson, JMF Jin, VL Hammerbeck, AL Varvel, GE Schumacher, TE AF Osborne, Shannon L. Johnson, Jane M. F. Jin, Virginia L. Hammerbeck, Amber L. Varvel, Gary E. Schumacher, Tom E. TI The Impact of Corn Residue Removal on Soil Aggregates and Particulate Organic Matter SO BIOENERGY RESEARCH LA English DT Article DE Bioenergy; Second generation feedstock; Sustainable; Renewable energy; Soil health ID STOVER REMOVAL; CARBON; TILLAGE; MANAGEMENT; QUALITY; SWITCHGRASS; STABILITY; BIOENERGY; ABUNDANCE AB Removal of corn (Zea mays L.) stover as a biofuel feedstock is being considered. It is important to understand the implications of this practice when establishing removal guidelines to ensure the long-term sustainability of both the biofuel industry and soil health. Aboveground and belowground plant residues are the soil's main sources of organic materials that bind soil particles together into aggregates and increase soil carbon (C) storage. Serving to stabilize soil particles, soil organic matter (SOM) assists in supplying plant available nutrients, increases water holding capacity, and helps reduce soil erosion. Data obtained from three Corn Stover Regional Partnership sites (Brookings, SD; Morris, MN; and Ithaca, NE) were utilized to evaluate the impact of removing corn stover on soil physical properties, including dry aggregate size distribution (DASD), erodible fraction (EF), and SOM components. Each site consisted of a combination of three residue removal rates (low-removal of grain only, intermediate-approximately 50 % residue removal, and high-maximum amount of residue removal). Results showed that the distribution of soil aggregates was less favorable for all three locations when residue was removed without the addition of other sources of organic matter such as cover crops. Additionally, we found that when residue was removed and the soil surface was less protected, there was an increase in the EF at all three research sites. There was a reduction in the EF for both the Brookings, SD, and Ithaca, NE sites when cover crops were incorporated or additional nitrogen (N) was added to the system. Amounts of SOM, fine particulate organic matter (fPOM), and total particulate organic matter (tPOM) consistently decreased as greater amounts of residue were removed from the soil surface. Across these three locations, the removal of crop residue from the soil surface had a negative impact on measured soil physical properties. The addition of a cover crop or additional N helped reduce this impact as measured through aggregate size distribution and EF and SOM components. C1 [Osborne, Shannon L.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Johnson, Jane M. F.] ARS, USDA, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Jin, Virginia L.; Varvel, Gary E.] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Hammerbeck, Amber L.; Schumacher, Tom E.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. RP Osborne, SL (reprint author), ARS, USDA, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM shannon.osborne@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Resilient Economic Agricultural Practices (REAP); North Central Regional Sun Grant Center at South Dakota State University through United States Department of Energy-Office of Biomass Programs [DE-FC36-05GO85041] FX Project funding was provided by the United States Department of Agriculture-Agricultural Research Service (USDA-ARS), as part of the USDA-ARS-Resilient Economic Agricultural Practices (REAP)/formally Renewable Energy Assessment Project. Additional funding was from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the United States Department of Energy-Office of Biomass Programs under award number DE-FC36-05GO85041. Technical assistance in the field and/or lab is acknowledged from Kurt Dagel, Chris Nelson, Ann Qualm, Gary Amundson, Nancy Barbour, Chad Rollofson, Stephan Swanson, Susan Siragusa, David Walla, Tyler Goeschel, Molly Hoffbauer, and Carla Ahlschwede. NR 30 TC 15 Z9 15 U1 9 U2 85 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 559 EP 567 DI 10.1007/s12155-014-9413-0 PG 9 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800011 ER PT J AU Archer, DW Karlen, DL Liebig, MA AF Archer, David W. Karlen, Douglas L. Liebig, Mark A. TI Crop Residue Harvest Economics: An Iowa and North Dakota Case Study SO BIOENERGY RESEARCH LA English DT Article DE Enterprise budgets; Feedstock costs; REAPnet analysis ID CORN STOVER; BIOMASS; FEEDSTOCK AB Rigorous economic analyses are crucial for the successful launch of lignocellulosic bioenergy facilities in 2014 and beyond. Our objectives are to (1) introduce readers to a query tool developed to use data downloaded from the Agricultural Research Service (ARS) REAPnet for constructing enterprise budgets and (2) demonstrate the use of the query tool with REAPnet data from two field research sites (Ames, IA, and Mandan, ND) for evaluating short-term economic performance of various biofuel feedstock production strategies. Our results for both sites showed that short-term (< 3 years) impacts on grain profitability were lower at lower average annual crop residue removal rates. However, it will be important to monitor longer term changes to see if grain profitability declines over time and if biomass harvest degrades soil resources. Analyses for Iowa showed short-term breakeven field-edge biomass prices of $26-$42 Mg-1 among the most efficient strategies, while results for North Dakota showed breakeven prices of $54-$73 Mg-1. We suggest that development of the data query tool is important because it helps illustrate several different soil and crop management strategies that could be used to provide sustainable feedstock supplies. C1 [Archer, David W.; Liebig, Mark A.] ARS, USDA, No Great Plains Res Lab, Mandan, ND 58554 USA. [Karlen, Douglas L.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA USA. RP Archer, DW (reprint author), ARS, USDA, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM david.archer@ars.usda.gov FU USDA-Agricultural Research Service as part of the USDA-ARS Resilient Economic Agricultural Practices (REAP) project; North Central Regional Sun Grant Center at South Dakota State University through US Department of Energy (DOE)-Bioenergy Technology Office (BETO) [DE-FC36-05GO85041] FX This research was funded by the USDA-Agricultural Research Service as part of the USDA-ARS Resilient Economic Agricultural Practices (REAP) project with additional funds from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Energy (DOE)-Office of Biomass Programs [now known as the Bioenergy Technology Office (BETO)] under award number DE-FC36-05GO85041. NR 20 TC 1 Z9 1 U1 2 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 568 EP 575 DI 10.1007/s12155-014-9428-6 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800012 ER PT J AU Bonner, IJ Muth, DJ Koch, JB Karlen, DL AF Bonner, Ian J. Muth, David J., Jr. Koch, Joshua B. Karlen, Douglas L. TI Modeled Impacts of Cover Crops and Vegetative Barriers on Corn Stover Availability and Soil Quality SO BIOENERGY RESEARCH LA English DT Article DE Landscape planning; Landscape Environmental Assessment Framework (LEAF); Soil conservation; Soil quality; Bioenergy; Sustainable agriculture ID AGRICULTURAL RESIDUE REMOVAL; ORGANIC-CARBON; BIOMASS; TILLAGE; BIOENERGY; SUSTAINABILITY; NITROGEN; MANAGEMENT; SEDIMENT; EROSION AB Environmentally benign, economically viable, and socially acceptable agronomic strategies are needed to launch a sustainable lignocellulosic biofuel industry. Our objective was to demonstrate a landscape planning process that can ensure adequate supplies of corn (Zea mays L.) stover feedstock while protecting and improving soil quality. The Landscape Environmental Assessment Framework (LEAF) was used to develop land use strategies that were then scaled up for five U.S. Corn Belt states (Nebraska, Iowa, Illinois, Indiana, and Minnesota) to illustrate the impact that could be achieved. Our results show an annual sustainable stover supply of 194 million Mg without exceeding soil erosion T values or depleting soil organic carbon [i.e., soil conditioning index (SCI) > 0] when no-till, winter cover crop, and vegetative barriers were incorporated into the landscape. A second, more rigorous conservation target was set to enhance soil quality while sustainably harvesting stover. By requiring erosion to be < 1/2 T and the SCI-organic matter (OM) subfactor to be > 0, the annual sustainable quantity of harvestable stover dropped to148 million Mg. Examining removal rates by state and soil resource showed that soil capability class and slope generally determined the effectiveness of the three conservation practices and the resulting sustainable harvest rate. This emphasizes that sustainable biomass harvest must be based on subfield management decisions to ensure soil resources are conserved or enhanced, while providing sufficient biomass feedstock to support the economic growth of bioenergy enterprises. C1 [Bonner, Ian J.] US DOE, Idaho Natl Lab Dept Biofuels & Renewable Energy T, Idaho Falls, ID 83415 USA. [Muth, David J., Jr.; Koch, Joshua B.] Praxik LLC, Ames, IA 50010 USA. [Karlen, Douglas L.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Bonner, IJ (reprint author), US DOE, Idaho Natl Lab Dept Biofuels & Renewable Energy T, POB 1625, Idaho Falls, ID 83415 USA. EM ian.bonner@inl.gov FU U.S. Department of Energy, under DOE Idaho Operations Office [DE-AC07-05ID14517] FX This work is supported by the U.S. Department of Energy, under DOE Idaho Operations Office Contract DE-AC07-05ID14517. Accordingly, the U.S. Government retains a nonexclusive, royalty-free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes. The authors also acknowledge Dr. Wally Tyner, Michelle Pratt, and Alicia English from Purdue University for their collaboration designing the conservation management strategies implemented in this analysis. NR 43 TC 15 Z9 15 U1 8 U2 50 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 576 EP 589 DI 10.1007/s12155-014-9423-y PG 14 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800013 ER PT J AU Cantrell, KB Novak, JM Frederick, JR Karlen, DL Watts, DW AF Cantrell, Keri B. Novak, Jeffrey M. Frederick, James R. Karlen, Douglas L. Watts, Donald W. TI Influence of Corn Residue Harvest Management on Grain, Stover, and Energy Yields SO BIOENERGY RESEARCH LA English DT Article DE Higher heating value; Residue removal; Crop management; Zea mays; Renewable Energy Assessment Project (REAP); Bioenergy; Combustion ID SOIL; BIOMASS; REMOVAL; CROP AB Economic, environmental, and energy independence issues are contributing to rising fossil fuel prices, petroleum supply concerns, and a growing interest in biomass feedstocks as renewable energy sources. Potential feedstocks include perennial grasses, timber, and annual grain crops with our focus being on corn (Zea mays L.) stover. A plot-scale study evaluating stover removal was initiated in 2008 on a South Carolina Coastal Plain Coxville/Rains-Goldsboro-Lynchburg soil association site. In addition to grain and stover yields, carbon balance, greenhouse gas (GHG) emissions and soil quality impact reported elsewhere in this issue, variation in gross energy distribution within various plant fractions - whole plant, below ear shank (bottom), above ear shank (top), cob, as well as leaves and stems of the bottom and top portions (n ((part, year)) = 20) was measured with an isoperibol calorimeter. Stalks from above the ear shank were the most energy dense, averaging 18.8 MJ/kg db, and when combined with other plant parts from above the ear shank, the entire top half was more energy dense than the bottom half - 18.4 versus 18.2 MJ/kg db. Gross energy content of the whole plant, including the cob, averaged 18.28 +/- 0.76 MJ/kg db. Over the 4 years, partial to total removal (i.e., 25 % to 100 %) of above-ground plant biomass could supply between 30 and 168 GJ/ha depending upon annual rainfall. At 168 GJ/ha, the quantity of corn stover biomass (whole plant) available in a 3,254-km(2) area (32 km radius) around the study site could potentially support a 500-MW power plant. C1 [Cantrell, Keri B.; Novak, Jeffrey M.; Watts, Donald W.] ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, Florence, SC 29501 USA. [Frederick, James R.] Clemson Univ, Pee Dee Res & Educ Ctr, Florence, SC 29501 USA. [Karlen, Douglas L.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Novak, JM (reprint author), ARS, Coastal Plains Soil Water & Plant Res Ctr, USDA, 2611 West Lucas St, Florence, SC 29501 USA. EM jeff.novak@ars.usda.gov FU USDA-Agricultural Research Service as part of the USDA-ARS-REAP project; North Central Regional SunGrant Center at South Dakota State University through USDOE - Office of Biomass Programs [DE-FC36-05GO85041] FX The authors thank the many hands involved with field and sample collection, sample preparation, and sample analyses: Chuck Parker, Karen Barefoot, Sheneeka Sansbury, Ashley Kelley, Elizabeth Ford, Brittney Wallace, Cierra Buckman, Joe Millen, and Jerry Martin II. The authors thank Dr. Jane Johnson and the journal reviewers for their input into the revisions. The authors would like to acknowledge the following for funding: USDA-Agricultural Research Service as part of the USDA-ARS-REAP project. Additional funding sources were with the North Central Regional SunGrant Center at South Dakota State University through a grant provided by the USDOE - Office of Biomass Programs under award number DE-FC36-05GO85041. NR 23 TC 4 Z9 4 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 590 EP 597 DI 10.1007/s12155-014-9433-9 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800014 ER PT J AU Huggins, DR Kruger, CE Painter, KM Uberuaga, DP AF Huggins, David R. Kruger, Chad E. Painter, Kathleen M. Uberuaga, David P. TI Site-Specific Trade-offs of Harvesting Cereal Residues as Biofuel Feedstocks in Dryland Annual Cropping Systems of the Pacific Northwest, USA SO BIOENERGY RESEARCH LA English DT Article DE Wheat residue; Biofuels; Cereal straw; Ethanol; Soil quality; Soil carbon ID SOIL ORGANIC-CARBON; CORN STOVER; BIOETHANOL PRODUCTION; ETHANOL-PRODUCTION; LAND-USE; BIOENERGY; DYNAMICS; IMPACTS; REMOVAL; EROSION AB Cereal residues are considered an important feedstock for future biofuel production. Harvesting residues, however, could lead to serious soil degradation and impaired agroecosystem services. Our objective was to evaluate trade-offs of harvesting wheat and barley residues including impacts on soil erosion and quality, soil organic C (SOC), and nutrient removal. We used agricultural data from 369 geo-referenced points on the 37-ha Washington State University Cook Agronomy Farm combined with model simulations to develop straw harvest scenarios for conventional tillage (CT) and no-tillage (NT) and both 2- and 3-year crop rotations with sequences of wheat, barley, and peas. Site-specific estimates of ethanol production from 2- and 3-year rotation scenarios ranged from 681 to 1,541 L ha(-1) yr(-1), indicating that both crop rotation and site-specific targeting of residue harvest are important factors. Harvesting straw reduced residue C inputs by 46 % and resulted in levels below that required to maintain SOC under CT. This occurred as a function of both straw harvest and low residue producing crops in rotation. Harvesting straw under CT was predicted to reduce soil quality as Soil Conditioning Indices (SCIs) were negative throughout the field. In contrast, SCIs under NT were positive despite straw harvest. Replacement value of nutrients (N, P, K, S) removed in harvested straw averaged $14.54 Mg-1 dry straw and ranged from $36.04 to $80.30 ha(-1), while straw harvesting costs averaged $34.25 Mg-1, and the current (2014) market value of straw is $65 Mg-1. We concluded that substantial trade-offs exist in harvesting straw for biofuel, that trade-offs should be evaluated on a site-specific basis, and that support practices such as crop rotation, reduced tillage, and site-specific nutrient management need to be considered if residue harvest is to be sustainable. C1 [Huggins, David R.] Washington State Univ, USDA ARS, Land Management & Water Conservat Res Unit, Pullman, WA 99164 USA. [Kruger, Chad E.] Washington State Univ, Ctr Sustaining Agr & Nat Resources, Wenatchee, WA 98801 USA. [Painter, Kathleen M.] Univ Idaho, Moscow, ID 83844 USA. [Uberuaga, David P.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Huggins, DR (reprint author), Washington State Univ, USDA ARS, Land Management & Water Conservat Res Unit, 215 Johnson Hall, Pullman, WA 99164 USA. EM dhuggins@wsu.edu; cekruger@wsu.edu; kpainter@uidaho.edu; duberuaga@wsu.edu FU Paul G. Allen Family Foundation; USDA Solutions to Economic and Environmental Problems (STEEP); USDA National Institute for Food and Agriculture [2011-68002-30191, 2011-67003-30341] FX This study was conducted as part of the Climate Friendly Farming Project with funding provided by the Paul G. Allen Family Foundation. Additional support was provided through the USDA Solutions to Economic and Environmental Problems (STEEP) as well as the projects "Regional Approaches to Climate Change for Pacific Northwest Agriculture" (REACCH) and "Site-Specific Climate-Friendly Farming" (SCF) funded through awards #2011-68002-30191 and #2011-67003-30341, respectively, from the USDA National Institute for Food and Agriculture. NR 57 TC 4 Z9 4 U1 2 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2014 VL 7 IS 2 BP 598 EP 608 DI 10.1007/s12155-014-9438-4 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA AG4OW UT WOS:000335400800015 ER PT J AU Wheeler, GS Chawner, M Williams, DA AF Wheeler, G. S. Chawner, M. Williams, D. A. TI Predicting the host range of Nystalea ebalea: Secondary plant chemistry and host selection by a surrogate biological control agent of Schinus terebinthifolia SO BIOLOGICAL CONTROL LA English DT Article DE Schinus terebinthifolia; Nutritional quality; Biological control of weeds; Herbivory; Urushiols; Toxic compounds; Volatiles ID BRAZILIAN PEPPERTREE; SAPINDALES ANACARDIACEAE; MELALEUCA-QUINQUENERVIA; OXYOPS-VITIOSA; LEPIDOPTERA NOTODONTIDAE; SOUTH FLORIDA; ESSENTIAL OIL; POISON-IVY; INSECTS; CHLOROPLAST AB The safety of weed biological control depends upon the selection and utilization of the target weed by the agent while causing minimal harm to non-target species. Selection of weed species by biological control agents is determined by the presence of behavioral cues, generally host secondary plant compounds that elicit oviposition and feeding responses. Non-target species that possess the same behavioral cues as found in the target weed may be at risk of damage by classical biological control agents. Here we conducted host range tests and examined secondary plant compounds of several test plant species. We studied the specialist herbivore Nystalea ebalea (Lepidoptera: Notodontidae) a Neotropical species, present in Florida as a surrogate biological control agent of the weed, Brazilian peppertree Schinus terebinthifolia, invasive in Florida and Hawaii. We found that the larvae had the greatest survival when fed the target weed, the Neotropical species Spondias purpurea, the Florida native species Rhus copallinum, and the ornamental Pistacia chinensis. Reduced survival and general larval performance were found on the native species Metopium toxiferum and Toxicodendron radicans. Both the volatiles and the allergen urushiols were chemically characterized for all species but urushiol diversity and concentration best predicted host range of this herbivore species. These results provide insight into host selection and utilization by one oligophagous Schinus herbivore. Other potential biological control agents may also be sensitive to plants that contain urushiols and if so, they may pose minimal risk to these native species. Published by Elsevier Inc. C1 [Wheeler, G. S.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Chawner, M.] Univ Wisconsin, Dept Biol, Madison, WI 53706 USA. [Williams, D. A.] Texas Christian Univ, Dept Biol, Ft Worth, TX 76129 USA. RP Wheeler, GS (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM greg.wheeler@ars.usda.gov RI Williams, Dean/H-6108-2016 OI Williams, Dean/0000-0002-9001-6019 FU Florida Fish and Wildlife Federation; South Florida Water Management District; USDA/ARS FX We thank M. Roddick (SCA/AmeriCorps), K. Dyer (USDA-ARS-IPRL) for technical assistance, Abdo Zaid, USDA-ARS, Athens, GA, USA and three anonymous reviewers who provided insightful comments on a earlier draft. Insect identifications were provided by M. Pogue (USDA-ARS-SEL, Beltsville, MD). This project was partially funded by Florida Fish and Wildlife Federation, South Florida Water Management District, and USDA/ARS. NR 91 TC 4 Z9 4 U1 2 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2014 VL 73 BP 39 EP 49 DI 10.1016/j.biocontrol.2014.02.017 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AG6GF UT WOS:000335516400007 ER PT J AU Morris, EE Hajek, AE Zieman, E Williams, DW AF Morris, E. Erin Hajek, Ann E. Zieman, Elliott Williams, David W. TI Deladenus (Tylenchida: Neotylenchidae) reproduction on species and strains of the white rot fungus Amylostereum SO BIOLOGICAL CONTROL LA English DT Article DE Deladenus; Amylostereum; Sirex noctilio; Biological control; Mycophagy; Diet breadth ID BIOLOGICAL-CONTROL AGENT; SIREX-NOCTILIO; SOUTHERN-HEMISPHERE; SIRICID WOODWASPS; NORTH-AMERICA; NEMATODES; AREOLATUM AB Deladenus siricidicola nematodes are used for the biological control of invasive Sirex noctilio woodwasps in the Southern Hemisphere. Since the discovery of established S. noctilio in North America and Canada in 2005, a similar biological control program is under consideration in the United States. In this study, a culture of Deladenus nematodes was established from a native Sirex nigricornis woodwasp collected in New York State. These nematodes were identified as Deladenus proximus using molecular and morphometric techniques. D. siricidicola Kamona are mass produced for biological control when feeding on the white rot fungus Amylostereum areolatum in the Southern Hemisphere, so we compared the relative development and reproduction of mycophagous forms of both nematode species when feeding on native and invasive strains of Amylostereum fungus. D. siricidicola Kamona were able to reproduce on all strains of A. areolatum tested, but reproduced poorly on the A. areolatum strain they would be most likely to encounter in northeastern North America, should the nematode be released. Multiple strains of both species of Amylostereum were observed to grow over nematode eggs, preventing them from hatching. D. proximus were able to reproduce well on both Amylostereum chailletii and A. areolatum, despite prior evidence suggesting only A. chailletii is a suitable food source. This is the first report of the ability of D. proximus to survive and reproduce on A. areolatum, the fungal symbiont usually carried by S. noctilio, suggesting this native nematode should be evaluated for its ability to parasitize and sterilize S. noctilio. (C) 2014 Elsevier Inc. All rights reserved. C1 [Morris, E. Erin; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Zieman, Elliott] So Illinois Univ, Dept Zool, Carbondale, IL 62901 USA. [Williams, David W.] USDA, APHIS, CPHST, Buzzards Bay, MA 02542 USA. RP Morris, EE (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM eem62@cornell.edu FU USDA APHIS [09-8100-1224 -CA] FX We greatly appreciate the help of Dr. Agustin Jimenez for help with nematode morphometrics and StBDF due to the authors' efan Long for helping to provide S. noctilio and S. nigricornis samples. We are also grateful for funding provided by USDA APHIS Project #09-8100-1224 -CA. NR 31 TC 6 Z9 6 U1 2 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2014 VL 73 BP 50 EP 58 DI 10.1016/j.biocontrol.2014.03.002 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AG6GF UT WOS:000335516400008 ER PT J AU Bryant, A Coudron, T Brainard, D Szendrei, Z AF Bryant, Alexandria Coudron, Thomas Brainard, Daniel Szendrei, Zsofia TI Cover crop mulches influence biological control of the imported cabbageworm (Pieris rapae L., Lepidoptera: Pieridae) in cabbage SO BIOLOGICAL CONTROL LA English DT Article DE Cotesia rubecula; Hippodamia convergens; Podisus maculiventris; Y-tube; Habitat management; Strip-tillage ID NATURAL ENEMY ASSOCIATION; DIAMONDBACK MOTH LEPIDOPTERA; PLUTELLA-XYLOSTELLA L.; COTESIA-RUBECULA; TRITROPHIC SYSTEM; BRASSICACEAE CRUCIFERAE; GENERALIST PREDATORS; SOYBEAN APHID; PEST-CONTROL; RYE MULCH AB Increasing structural complexity within crop fields can provide a way to manipulate pest abundance and biological control in agroecosystems. Here, we examine the effect of cover crop mulches in cabbage on the structure and function of an insect food web, investigating the role of cover crop species, structure, and volatile cues on important interactions. We focused on the imported cabbageworm (Pieris rapae L., Lepidoptera: Pieridae), and three of its natural enemies, the spined soldier bug (Podisus maculiventris (Say), Hemiptera: Pentatomidae), the convergent lady beetle (Hippodamia convergens (Guerin), Coleoptera: Coccinellidae), and the parasitoid, Cotesia rubecula Marshall (Hymenoptera: Braconidae). We measured the abundance of these insects in a field experiment and conducted a natural enemy exclusion cage study to determine the level of biological control of the imported cabbageworm in the field. Our field experiments indicated that cover crop species, but not structure, influenced insect abundance, with significantly more imported cabbageworm and C. rubecula in rye cover crop mulch plots compared to vetch mulch or bare soil plots. In the Y-tube assays we found some evidence that the increased parasitoid abundance did not result in increased parasitism because of interference of the mulch with short-range host finding odor cues. The natural abundance of the two predators was not different among our field plots with different cover crop treatments. Mortality and parasitism of sentinel imported cabbageworm larvae was not different in field cages among the different cover crop mulch treatments, but there was a significant difference among cage types indicating that small natural enemies play an important role in the biological control of this cabbage pest. (C) 2014 Elsevier Inc. All rights reserved. C1 [Bryant, Alexandria; Szendrei, Zsofia] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Coudron, Thomas] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Brainard, Daniel] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. RP Szendrei, Z (reprint author), Michigan State Univ, Dept Entomol, 288 Farm Lane,Room 243, E Lansing, MI 48824 USA. EM szendrei@msu.edu NR 57 TC 3 Z9 3 U1 6 U2 60 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2014 VL 73 BP 75 EP 83 DI 10.1016/j.biocontrol.2014.03.012 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AG6GF UT WOS:000335516400011 ER PT J AU Kang, JW AF Kang, Jun Won TI Removing environmental organic pollutants with bioremediation and phytoremediation SO BIOTECHNOLOGY LETTERS LA English DT Review DE Bioremediation; Chlorinated hydrocarbons; Endophytes; Environmental biotechnology; Organochlorides; Phytoremediation; Transgenic plants ID PSEUDOMONAS-CEPACIA G4; MAMMALIAN CYTOCHROME-P450 2E1; ATRAZINE-CONTAMINATED SOIL; O-XYLENE MONOOXYGENASE; COMETABOLIC DEGRADATION; TRANSGENIC PLANTS; HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE RDX; TRICHLOROETHYLENE DEGRADATION; ENHANCED PHYTOREMEDIATION; CHLORINATED SOLVENTS AB Hazardous organic pollutants represent a threat to human, animal, and environmental health. If left unmanaged, these pollutants could cause concern. Many researchers have stepped up efforts to find more sustainable and cost-effective alternatives to using hazardous chemicals and treatments to remove existing harmful pollutants. Environmental biotechnology, such as bioremediation and phytoremediation, is a promising field that utilizes natural resources including microbes and plants to eliminate toxic organic contaminants. This technology offers an attractive alternative to other conventional remediation processes because of its relatively low cost and environmentally-friendly method. This review discusses current biological technologies for the removal of organic contaminants, including chlorinated hydrocarbons, focusing on their limitation and recent efforts to correct the drawbacks. C1 [Kang, Jun Won] Univ Washington, Coll Environm, Sch Environm & Forest Sci, Seattle, WA 98195 USA. RP Kang, JW (reprint author), ARS, Natl Germplasm Resources Lab, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM jwkang75@uw.edu NR 107 TC 16 Z9 18 U1 37 U2 240 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD JUN PY 2014 VL 36 IS 6 BP 1129 EP 1139 DI 10.1007/s10529-014-1466-9 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AG0ZY UT WOS:000335145800002 PM 24563299 ER PT J AU Zelinka, SL Glass, SV Derome, D AF Zelinka, Samuel L. Glass, Samuel V. Derome, Dominique TI The effect of moisture content on the corrosion of fasteners embedded in wood subjected to alkaline copper quaternary treatment SO CORROSION SCIENCE LA English DT Article DE Steel; Zinc; Polarization; Weight loss; X-ray diffraction ID POLARIZATION RESISTANCE MEASUREMENTS; AQUEOUS TIMBER PRESERVATIVES; ZINC-BASED MATERIALS; ALLOY COATED STEEL; TREATED WOOD; THREADED FASTENER; TAFEL SLOPES; SURFACE-AREA; RATES; ENVIRONMENTS AB This paper characterizes the corrosion rate of embedded fasteners as a function of wood moisture content using gravimetric and electrochemical measurements. The results indicated that the corrosion rate increased with moisture content before reaching a plateau. The phases present in the corrosion products, as analyzed using X-ray diffraction, are generally consistent with previous work. Uniform corrosion was observed for all fasteners and all conditions except steel fasteners embedded in water-saturated wood. Data of dependence of corrosion rate on moisture content, presented herein, are necessary to ensure the accuracy of combined hygrothermal/corrosion models used to predict durability of wood structures. Published by Elsevier Ltd. C1 [Zelinka, Samuel L.; Glass, Samuel V.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Derome, Dominique] Swiss Fed Inst Technol Zurich ETHZ, Chair Bldg Phys, CH-8093 Zurich, Honggerberg, Switzerland. RP Zelinka, SL (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM szelinka@fs.fed.us NR 46 TC 3 Z9 3 U1 1 U2 13 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0010-938X EI 1879-0496 J9 CORROS SCI JI Corrosion Sci. PD JUN PY 2014 VL 83 BP 67 EP 74 DI 10.1016/j.corsci.2014.01.044 PG 8 WC Materials Science, Multidisciplinary; Metallurgy & Metallurgical Engineering SC Materials Science; Metallurgy & Metallurgical Engineering GA AG2VH UT WOS:000335274700007 ER PT J AU Cen, HY Lu, RF Ariana, DP Mendoza, F AF Cen, Haiyan Lu, Renfu Ariana, Diwan P. Mendoza, Fernando TI Hyperspectral Imaging-Based Classification and Wavebands Selection for Internal Defect Detection of Pickling Cucumbers SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Hyperspectral imaging; Reflectance; Transmittance; Defect detection; Grading; Minimum redundancy-maximum relevance; LED light ID NEAR-INFRARED-SPECTROSCOPY; SOLUBLE SOLIDS CONTENT; NIR SPECTROSCOPY; SYSTEM; FRUIT; IDENTIFICATION; TRANSMISSION; FIRMNESS; QUALITY; VISION AB Hyperspectral imaging is useful for detecting internal defects of pickling cucumbers. The technique, however, is not yet suitable for high-speed online implementation due to the challenges in analyzing large-scale hyperspectral images. This research aimed to select the optimal wavebands from the hyperspectral image data, so that they can be deployed in either a hyperspectral or multispectral imaging-based inspection system for the automatic detection of internal defects of pickling cucumbers. Hyperspectral reflectance (400-700 nm) and transmittance (700-1,000 nm) images were acquired, using an in-house developed hyperspectral imaging system running at two conveyor speeds of 85 and 165 mm/s, for 300 "Journey" pickling cucumbers before and after internal damage was induced by mechanical load. Minimum redundancy-maximum relevance (MRMR) was used for optimal wavebands selection, and the loadings of principal component analysis (PCA) were also applied for qualitatively identifying the important wavebands that are related to the specific features. Discriminant analysis with Mahalanobis distance classifier was performed for the two-class (i.e., normal and defective) and three-class (i.e., normal, slightly defective, and severely defective) classifications using the mean spectra and textural features (energy and variance) from the regions of interest in the spectral images at selected waveband ratios. The classification results based on MRMR wavebands selection were generally better than those from PCA-based classifications. The two-band ratio of 887/837 nm from MRMR gave the best overall classification results, with the accuracy of 95.1 and 94.2 % at the conveyor speeds of 85 and 165 mm/s, respectively, for the two-class classification. The highest classification accuracies for the three-class classification based on the optimal two-band ratio of 887/837 nm were 82.8 and 81.3 % at the conveyor speeds of 85 and 165 mm/s, respectively. The mean spectra-based classification achieved better results than the textural feature-based classification, except in the three-class classification for the higher conveyor speed. The overall classification accuracies for all selected waveband ratios at the low conveyor speed were slightly higher than those at the higher conveyor speed, since the low speed resulted in more scan lines, thus higher spatial resolution hyperspectral images. The identified two-band ratio of 887/837 nm in transmittance mode could be applied for fast real-time internal defect detection of pickling cucumbers. C1 [Cen, Haiyan; Ariana, Diwan P.] Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. [Lu, Renfu; Mendoza, Fernando] Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. RP Cen, HY (reprint author), Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. EM cenhaiya@msu.edu; renfu.lu@ars.usda.gov NR 41 TC 18 Z9 18 U1 6 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD JUN PY 2014 VL 7 IS 6 BP 1689 EP 1700 DI 10.1007/s11947-013-1177-6 PG 12 WC Food Science & Technology SC Food Science & Technology GA AG1BD UT WOS:000335149000013 ER PT J AU Li, X Zhang, A Atungulu, G McHugh, T Delwiche, M Lin, S Zhao, LM Pan, ZL AF Li, Xuan Zhang, Ang Atungulu, Griffiths McHugh, Tara Delwiche, Michael Lin, Shuang Zhao, Liming Pan, Zhongli TI Characterization and Multivariate Analysis of Physical Properties of Processing Peaches SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Clingstone peach; Physical properties; Quality; Processing; Multivariate analyses ID PERSICA L. BATSCH; QUALITY ATTRIBUTES; CLINGSTONE PEACHES; FRUIT-QUALITY; ORIENTATION; VARIETIES; TOMATOES; COLOR; SHAPE AB Characterization of physical properties of fruits represents the first vital step to ensure optimal performance of fruit processing operations and is also a prerequisite in the development of new processing equipment. In this study, physical properties of engineering significance to processing of three popular cultivars of clingstone peaches were evaluated, including dimensional parameters, mass, dimensional ratios, aspect ratio, elongation index, sphericity, bulk density, texture, color, and flavor. Based on these physical properties, multivariate analysis of variance (MANOVA), canonical variate analysis (CVA), principal component analysis (PCA), and partial least squares and linear discriminate analysis (PLS-LDA) were applied to qualitatively and quantitatively discriminate the cultivar difference. Results showed that the studied peach cultivars had significantly different (p < 0.05) geometric characteristics. The peaches can be classified based on the cheek diameter (D-c) into three different size categories, including small- (D-c less than 60 mm), medium- (D-c between 60 mm and 70 mm), and large- (D-c higher than 70 mm) sized peaches. The peach flesh firmness significantly (p < 0.05) decreased with the increase of peach size, while the pit dimensions were independent of peach size. There were no apparent distinctions in color characteristics, bulk density, and sugar content among the three cultivars. The measurements and quantitative discrimination of peach properties in this study would benefit equipment design and process innovation to enhance the processing efficiency and quality of processed peaches. C1 [Li, Xuan; Zhang, Ang; Atungulu, Griffiths; Delwiche, Michael; Lin, Shuang; Zhao, Liming; Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Zhang, Ang] Northwest Agr & Forestry Univ, Coll Enol, Yangling 712100, Shanxi, Peoples R China. [Atungulu, Griffiths] Univ Arkansas, Dept Food Sci, Fayetteville, AR 72704 USA. [Atungulu, Griffiths] Univ Arkansas, Div Agr, Fayetteville, AR 72704 USA. [McHugh, Tara; Pan, Zhongli] ARS, Professed Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Lin, Shuang] Univ Calif Berkeley, Dept Comp Sci, Berkeley, CA 94720 USA. [Zhao, Liming] E China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China. RP Pan, ZL (reprint author), ARS, Professed Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM zhaoliming@ecust.edu.cn; zhongli.pan@ars.usda.gov RI Li, Xuan/J-5786-2015 FU USDA-ARS Cooperative Research and Development Agreement (CRADA) project FX This research work was funded by the USDA-ARS Cooperative Research and Development Agreement (CRADA) project. The authors gratefully acknowledge Rebecca Milczarek from the USDA-ARS and Moy Michelle, James Valenti-Jordan, and Amy Schauwecker from the Del Monte Food Incorporation for their support in this study. NR 34 TC 5 Z9 5 U1 4 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD JUN PY 2014 VL 7 IS 6 BP 1756 EP 1766 DI 10.1007/s11947-014-1269-y PG 11 WC Food Science & Technology SC Food Science & Technology GA AG1BD UT WOS:000335149000019 ER PT J AU Thompson, PC Rosenthal, BM Hare, MP AF Thompson, Peter C. Rosenthal, Benjamin M. Hare, Matthew P. TI Hybridization between previously isolated ancestors may explain the persistence of exactly two ancient lineages in the genome of the oyster parasite Perkinsus marinus SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Dimorphism; Hybridization; Perkinsus; Coalescent; Parasite; Evolution ID MEROZOITE SURFACE PROTEIN-1; PLASMODIUM-FALCIPARUM; CRASSOSTREA-VIRGINICA; CHESAPEAKE BAY; POPULATION-GENETICS; DIVERSITY; SEQUENCES; SOFTWARE; PATHOGEN; POLYMORPHISM AB Theory predicts that neutral genetic variation accumulates within populations to a level determined by gains through mutation and losses by genetic drift. This balance results in a characteristic distribution of allelic variation with the maximum allelic difference determined by effective population size. Here, we report a striking departure from these expectations in the form of allelic dimorphism, observed at the majority of seven loci examined in Perkinsus marinus, an important oyster parasite that causes Dermo disease. DNA sequences were collected from five loci flanking microsatellite repeats and two loci coding for superoxide dismutase enzymes that may mediate the parasite's interaction with its host. Based on 474 sequences, sampled across 5000 km of the eastern United States coastline, no more than two alleles were observed at each locus (discounting singletons). Depending on the locus, the common allele ranged in overall frequency from 72% to 92%. At each locus the two alleles differed substantially (3.8% sequence difference, on average), and the among-locus variance in divergences was not sufficient to reject a simultaneous origin for all dimorphisms using approximate Bayesian methods. Dimorphic alleles were estimated to have diverged from a common ancestral allele at least 0.9 million years ago. Across these seven loci, only five other alleles were ever observed, always as singletons and differing from the dimorphic alleles by no more than two nucleotides. Free recombination could potentially have shuffled these dimorphisms into as many as 243 multilocus combinations, but the existence of only ten combinations among all samples strongly supports low recombination frequencies and is consistent with the observed absence of intragenic recombination. We consider several demographic and evolutionary hypotheses to explain these patterns. Few can be conclusively rejected with the present data, but we advance a recent hybridization of ancient divergent lineages scenario as the most parsimonious. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. C1 [Thompson, Peter C.; Hare, Matthew P.] Univ Maryland, Dept Biol, College Pk, MD 20742 USA. [Rosenthal, Benjamin M.] USDA ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Thompson, PC (reprint author), USDA ARS, Anim Parasit Dis Lab, Bldg 1180 BARC East, Beltsville, MD 20705 USA. EM pete.c.thompson@gmail.com OI Rosenthal, Benjamin/0000-0002-0224-3773 FU Behavior, Ecology, Evolution, and Systematics Program at the University of Maryland College Park; U.S. Department of Agriculture FX We would like to thank Scott Roy for assistance in designing a formal test of neutrality based on the coalescent. We are also thankful to W. Pecher and G. Vasta for sharing samples collected in the northeastern portion of the range as well as multiple state agencies for collecting wild samples from the Gulf of Mexico and South Carolina. We would also like to thank the two anonymous reviewers whose comments greatly improved this manuscript. This publication was supported by the Behavior, Ecology, Evolution, and Systematics Program at the University of Maryland College Park and salary support to PCT from the U.S. Department of Agriculture. NR 60 TC 3 Z9 3 U1 3 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD JUN PY 2014 VL 24 BP 167 EP 176 DI 10.1016/j.meegid.2014.03.003 PG 10 WC Infectious Diseases SC Infectious Diseases GA AG4GG UT WOS:000335376800019 PM 24681265 ER PT J AU Radwan, O Rouhana, LV Hartman, GL Korban, SS AF Radwan, Osman Rouhana, Laura V. Hartman, Glen L. Korban, Schuyler S. TI Genetic Mechanisms of Host-Pathogen Interactions for Charcoal Rot in Soybean SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Review DE Charcoal rot; Drought stress; Genetic resistance; Soybean ID PROMOTE DISEASE DEVELOPMENT; DROUGHT-RESPONSIVE GENES; STEM-CELL MAINTENANCE; MACROPHOMINA-PHASEOLINA; ABIOTIC STRESS; HETERODERA-GLYCINES; CYST NEMATODES; WATER-DEFICIT; NECROTROPHIC PATHOGENS; FUSARIUM-VIRGULIFORME AB Soybean is a leading agronomic crop and contributes to food and agricultural security with expanding production areas in diverse regions around the world. Although soybean is challenged by several diseases and pests and progress has been made in understanding and managing some of these pathogens and pests, charcoal rot, incited by the soil-borne fungal pathogen Macrophomina phaseolina, has received little attention. M. phaseolina has a broad host range and is capable of attacking and infecting several groups of plant species, including soybean. Charcoal rot symptoms on soybean appear more commonly during hot and dry weather conditions, and are associated with drought stress. In recent years, it has become more important to develop management strategies to control charcoal rot in soybean fields. Understanding the genetics of this pathogen as well as its interactions with plant hosts will help in developing effective control and management strategies. The biology of M. phaseolina, its genetics, and plant-fungal relationships are reviewed herein. In addition, a discussion of potential opportunities utilizing modern tools to enhance genetic resistance against charcoal rot is also presented. C1 [Radwan, Osman; Rouhana, Laura V.; Hartman, Glen L.; Korban, Schuyler S.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Radwan, Osman] Zagazig Univ, Dept Plant Prod, Coll Technol & Dev, Sharkia, Egypt. [Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL USA. [Korban, Schuyler S.] Univ Massachusetts, Dept Biol, Boston, MA 02125 USA. RP Radwan, O (reprint author), Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. EM oradwan@illinois.edu NR 136 TC 4 Z9 4 U1 4 U2 42 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2014 VL 32 IS 3 BP 617 EP 629 DI 10.1007/s11105-013-0686-9 PG 13 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AG1ES UT WOS:000335158500001 ER PT J AU Stevanato, P Broccanello, C Biscarini, F Del Corvo, M Sablok, G Panella, L Stella, A Concheri, G AF Stevanato, Piergiorgio Broccanello, Chiara Biscarini, Filippo Del Corvo, Marcello Sablok, Gaurav Panella, Lee Stella, Alessandra Concheri, Giuseppe TI High-Throughput RAD-SNP Genotyping for Characterization of Sugar Beet Genotypes SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Sugar beet; Genetic diversity; SNP genotyping; QuantStudio platform ID BETA-VULGARIS; GENETIC DIVERSITY; LINKAGE DISEQUILIBRIUM; POLYMORPHISM; POPULATIONS; FREQUENCY; GERMPLASM; MARKERS; PLANTS; SCALE AB High-throughput single-nucleotide polymorphism (SNP) genotyping provides a rapid way of developing resourceful sets of markers for delineating genetic structure and for understanding the basis of the taxonomic discrimination. In this paper, we present a panel of 192 SNPs for effective genotyping in sugar beet using a high-throughput marker array technology, QuantStudio 12K Flex system, coupled with Taqman OpenArray technology. The selected SNPs were evaluated for genetic diversity among a set of 150 individuals representing 15 genotypes (10 individuals each) from five cytoplasmic male steriles (CMSs), five pollinators, and five commercial varieties. We demonstrated that the proposed panel of 192 SNPs effectively differentiated the studied genotypes. A higher degree of polymorphism was observed among the CMSs as compared to pollinators and commercial varieties. PCoA and STRUCTURE analysis revealed that CMSs, pollinators, and varieties clustered into three distinct subpopulations. Our results demonstrate the utility of the identified panel of 192 SNPs coupled with TaqMan OpenArray technology as a wide set of markers for high-throughput SNP genotyping in sugar beet. C1 [Stevanato, Piergiorgio; Broccanello, Chiara; Concheri, Giuseppe] Univ Padua, Dipartimento Agron Anim Alimenti Risorse Nat & Am, I-35020 Padua, Italy. [Biscarini, Filippo; Del Corvo, Marcello; Stella, Alessandra] FPTP, I-26900 Lodi, Italy. [Sablok, Gaurav] Fdn Edmund Mach, Dept Biodivers & Mol Ecol, I-38010 Trentino, Italy. [Panella, Lee] USDA ARS, Sugar Beet Res Unit, Crops Res Lab, NPA, Ft Collins, CO 80526 USA. RP Stevanato, P (reprint author), Univ Padua, Dipartimento Agron Anim Alimenti Risorse Nat & Am, Viale Univ 16, I-35020 Padua, Italy. EM stevanato@unipd.it RI Concheri, Giuseppe/L-4151-2015; Sablok, Gaurav/C-5940-2014 OI Concheri, Giuseppe/0000-0002-5812-8504; Sablok, Gaurav/0000-0002-4157-9405 NR 32 TC 7 Z9 7 U1 3 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2014 VL 32 IS 3 BP 691 EP 696 DI 10.1007/s11105-013-0685-x PG 6 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AG1ES UT WOS:000335158500007 ER PT J AU Zhou, XY Jenks, MA Liu, J Liu, AL Zhang, XW Xiang, JH Zou, J Peng, Y Chen, XB AF Zhou, Xiaoyun Jenks, Matthew A. Liu, Juan Liu, Ailing Zhang, Xiangwen Xiang, Jianhua Zou, Jie Peng, Yan Chen, Xinbo TI Overexpression of Transcription Factor OsWR2 Regulates Wax and Cutin Biosynthesis in Rice and Enhances its Tolerance to Water Deficit SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Plant cuticle; Drought tolerance; Wax; Cutin; Overexpression ID FATTY-ACID ELONGASE; CUTICULAR WAX; DROUGHT TOLERANCE; ARABIDOPSIS-THALIANA; FUNCTIONAL-CHARACTERIZATION; HETEROLOGOUS EXPRESSION; ALKANE BIOSYNTHESIS; EPICUTICULAR WAXES; BARRIER PROPERTIES; CONDENSING ENZYME AB Drought is the major abiotic stress limiting crop production. Plant cuticle represents the outer-most layer of the epidermis and plays an important role in decreasing plant water loss under drought stress by restricting non-stomatal transpiration. We report here that the Wax Synthesis Regulatory 2 gene (OsWR2) in rice (Oryza sativa L.) is highly expressed in epidermal tissues and contributes to the transcriptional regulation of both cuticular wax and cutin biosynthesis in rice cuticle. Overexpression of OsWR2 in rice increased the total cuticular wax level by 48.6 % in leaves and by 72.4 % in panicles. Of the major wax classes, aldehydes increased most in leaves, and alkanes increased most in panicles. Total cutin amounts were increased by 48.1 % in leaves and 65.9 % in panicles of rice overexpressing OsWR2, and these increases were due primarily to the increase in omega-OH and di-OH acids. Our results showed that 19 genes previously associated with wax and cutin biosynthesis were up-regulated in OsWR2 overexpressors. Overexpression of OsWR2 also altered cuticular wax crystallization and cuticle membrane ultrastructure. Furthermore, OsWR2 overexpression in rice decreased leaf chlorophyll leaching rate, reduced water loss rate, and enhanced tolerance to water-limited conditions. We demonstrate in this report that OsWR2 regulates wax and cutin biosynthesis differently than does the OsWR1 homologue, and plays a major role in controlling cuticle permeability. The increased resistance to water deficit conditions by OsWR2 overexpression in rice elucidates a potential new strategy for genetic improvement of plant drought tolerance. C1 [Zhou, Xiaoyun; Liu, Juan; Liu, Ailing; Zhang, Xiangwen; Xiang, Jianhua; Zou, Jie; Peng, Yan; Chen, Xinbo] Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat C, Changsha 410128, Hunan, Peoples R China. [Zhou, Xiaoyun; Liu, Ailing; Zhang, Xiangwen; Chen, Xinbo] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China. [Jenks, Matthew A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Chen, XB (reprint author), Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat C, Changsha 410128, Hunan, Peoples R China. EM xinbochen@live.cn RI Chen, Xinbo/J-6708-2012 OI Chen, Xinbo/0000-0002-8758-0591 FU Natural Science Foundation of China [30870206]; The Education Ministry Program for Innovative Research Team in University [IRT1239]; Hunan Agricultural University Science Foundation [09WD31]; Construct Program of the Key Discipline in Hunan Province FX This work was supported by Natural Science Foundation of China (30870206), The Education Ministry Program for Innovative Research Team in University (IRT1239), Hunan Agricultural University Science Foundation (09WD31) and the Construct Program of the Key Discipline in Hunan Province. NR 54 TC 6 Z9 8 U1 0 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2014 VL 32 IS 3 BP 719 EP 731 DI 10.1007/s11105-013-0687-8 PG 13 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AG1ES UT WOS:000335158500009 ER PT J AU Crain, BJ Tremblay, RL AF Crain, Benjamin J. Tremblay, Raymond L. TI Do richness and rarity hotspots really matter for orchid conservation in light of anticipated habitat loss? SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE rare plants; random forest models; Biodiversity hotspots; stochastic extinction models; Lepanthes (Orchidaceae); deforestation rates ID BIODIVERSITY HOTSPOTS; LEPANTHES ORCHIDACEAE; PLANT CONSERVATION; SPECIES RICHNESS; RANDOM FORESTS; PATTERNS; DIVERSITY; RARE; EXTINCTION; ENDEMISM AB Aim The aim of this analysis was to identify strategies that will maximize efficiency and effectiveness in conservation planning. As many orchids are threatened with extinction for various reasons, our primary objective was to combine hotspots analyses with stochastic extinction modelling to highlight possible conservation priorities for Lepanthes spp. (Orchidaceae) based on patterns of richness, rarity and threat. Our subsequent objective was to identify potential conservation surrogates and variables that are the best predictors of extinction probabilities. The ultimate goal was to determine which factors should be emphasized in conservation planning to prevent species extinctions. Location Latin America; the Caribbean. Methods We used herbarium records and ArcGIS to map the distribution of Lepanthes spp. and to identify hotspots of richness and rarity. We forecasted extinction patterns with Koopowitz's stochastic extinction model and calculated extinction probabilities in each country. We used a randomForest regression model in R to assess the importance of richness, rarity and threat for explaining extinction probabilities. Results Hotspots of Lepanthes richness and rarity occurred in north-western South America and southern Central America and largely overlapped with each other. The highest extinction probabilities occurred in northern Central America, Haiti and Ecuador, and generally, hotspots of richness and rarity did not correspond with patterns of threat. Habitat loss was the most important variable for explaining extinction probabilities, followed by measures of rarity. Main conclusions Conservation efforts will be most efficient in richness and rarity hotspots, and because they overlap, rarity hotspots could act as surrogates for protecting overall Lepanthes diversity. Hotspots rarely occurred in the most threatened areas, and therefore, conservation efforts are more urgent in non-hotspot areas. Conservation efforts will be most effective if they combine ex situ strategies in locations with high habitat conversion rates with reservation strategies in rarity and richness hotspots, particularly where they overlap. C1 [Crain, Benjamin J.; Tremblay, Raymond L.] Univ Puerto Rico Rio Piedras, Dept Biol, San Juan, PR 00936 USA. [Crain, Benjamin J.] US Forest Serv, Int Inst Trop Forestry, USDA, San Juan, PR 00926 USA. [Tremblay, Raymond L.] Univ Puerto Rico, Ctr Appl Trop Ecol & Conservat, Dept Biol, San Juan, PR 00936 USA. RP Crain, BJ (reprint author), Univ Puerto Rico Rio Piedras, Dept Biol, POB 23360, San Juan, PR 00936 USA. EM bcrainium@yahoo.com FU Circulo de Amigos de Orquidistas de Puerto Rico FX We sincerely thank our families and friends for their ongoing support. Additional thanks to members of the IITF GIS and Remote Sensing Laboratory for assisting with spatial analyses. We are grateful to employees of the UPR herbarium, the Estacion Experimental de Orquideas de la Familia Archila, Lankester Botanical Gardens, the Alexander von Humboldt Institute for Research on Biological Resources, and the UW herbarium for providing access to valuable resources and distribution data. We also thank A. Sanchez-Cuervo for continual backing. Lastly, we genuinely thank the Circulo de Amigos de Orquidistas de Puerto Rico for providing financial support. NR 69 TC 9 Z9 9 U1 4 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD JUN PY 2014 VL 20 IS 6 BP 652 EP 662 DI 10.1111/ddi.12179 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AF7KL UT WOS:000334893900004 ER PT J AU Sanderson, MA AF Sanderson, Matt A. TI Evaluating the USDA-NRCS pasture condition score system with weighted indicators SO ECOLOGICAL INDICATORS LA English DT Article DE Cost-share programs; Pasture assessment; Plant vigor; Plant residue; Standard reference site AB The pasture condition score (PCS) system is a monitoring and assessment tool for pastureland in the USA. The original system has been modified to address specific variations in vegetation and soils by assigning weights to the individual indicators to emphasize or de-emphasize particular attributes. We compared the effects of different weighting systems on aggregate PCSs and their distribution among five management recommendation categories. Five regional weighting systems were used to compute aggregate PCSs from a data set of 178 observations of pasture condition indicators measured on 39 farms. Distributions of PCSs from the five systems were compared with those obtained from using the original system. In modifications of the original system, plant vigor was the most heavily weighted indicator, whereas plant residue was the least weighted. The various weighting methods tended to increase aggregate scores compared with nonweighted scores, which resulted in different management recommendations. The median score for the original system was 31 compared with a range of 33 to 36 for the weighted systems. Alterations of the PCS system to include weighted indicators attempt to adjust for different environments and site types and suggest the need for some form of standard reference site or classification to account for differences in site type. Published by Elsevier Ltd. C1 USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Sanderson, MA (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM matt.sanderson@ars.usda.gov FU Conservation Effects Assessment Project (CEAP) - USDA Natural Resources Conservation Service; Conservation Effects Assessment Project (CEAP) - Agricultural Research Service FX We thank the farmers who allowed us to come on their farm to conduct this research. Their hospitality and interest is much appreciated. Kevin Ogles of the USDA-NRCS East National Technology Support Center and Ken Spaeth of the USDA-NRCS Central National Technology Support Center Staff provided reviews of an earlier version of this manuscript. This work was supported by funds from a Conservation Effects Assessment Project (CEAP) jointly funded by the USDA Natural Resources Conservation Service and the Agricultural Research Service. NR 10 TC 2 Z9 2 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD JUN PY 2014 VL 41 BP 183 EP 186 DI 10.1016/j.ecolind.2014.01.042 PG 4 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AF6JU UT WOS:000334822200021 ER PT J AU Neill, JD Bayles, DO Ridpath, JF AF Neill, John D. Bayles, Darrell O. Ridpath, Julia F. TI Simultaneous rapid sequencing of multiple RNA virus genomes SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Bovine viral diarrhea virus; Bovine coronavirus; Ion Torrent; Genome assembly; Next generation sequencing ID MAMMALIAN VIRUSES; IDENTIFICATION; VIROME; CHILDREN; SAMPLES; PIGS AB Comparing sequences of archived viruses collected over many years to the present allows the study of viral evolution and contributes to the design of new vaccines. However, the difficulty, time and expense of generating full-length sequences individually from each archived sample have hampered these studies. Next generation sequencing technologies have been utilized for analysis of clinical and environmental samples to identify viral pathogens that may be present. This has led to the discovery of many new, uncharacterized viruses from a number of viral families. Use of these sequencing technologies would be advantageous in examining viral evolution. In this study, a sequencing procedure was used to sequence simultaneously and rapidly multiple archived samples using a single standard protocol. This procedure utilized primers composed of 20 bases of known sequence with 8 random bases at the 3'-end that also served as an identifying barcode that allowed the differentiation each viral library following pooling and sequencing. This conferred sequence independence by random priming both first and second strand cDNA synthesis. Viral stocks were treated with a nuclease cocktail to reduce the presence of host nucleic acids. Viral RNA was extracted, followed by single tube random-primed double-stranded cDNA synthesis. The resultant cDNAs were amplified by primer-specific PCR, pooled, size fractionated and sequenced on the Ion Torrent PGM platform. The individual virus genomes were readily assembled by both de novo and template-assisted assembly methods. This procedure consistently resulted in near full length, if not full-length, genomic sequences and was used to sequence multiple bovine pestivirus and coronavirus isolates simultaneously. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/). C1 [Neill, John D.; Ridpath, Julia F.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. [Bayles, Darrell O.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Neill, JD (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM john.neill@ars.usda.gov NR 20 TC 17 Z9 18 U1 1 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD JUN 1 PY 2014 VL 201 BP 68 EP 72 DI 10.1016/j.jviromet.2014.02.016 PG 5 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA AF8QA UT WOS:000334979500012 PM 24589514 ER PT J AU Liebman, M Miller, ZJ Williams, CL Westerman, PR Dixon, PM Heggenstaller, A Davis, AS Menalled, FD Sundberg, DN AF Liebman, M. Miller, Z. J. Williams, C. L. Westerman, P. R. Dixon, P. M. Heggenstaller, A. Davis, A. S. Menalled, F. D. Sundberg, D. N. TI Fates of Setaria faberi and Abutilon theophrasti seeds in three crop rotation systems SO WEED RESEARCH LA English DT Article DE velvet leaf; crop rotation; seed mortality; seedling emergence; giant foxtail; seedbank dynamics; weeds ID WEED SEEDBANK; GIANT FOXTAIL; INTEGRATED MANAGEMENT; WOOLLY CUPGRASS; VELVETLEAF; PREDATION; PERSISTENCE; DYNAMICS; TILLAGE; TARGET AB Weed seeds in and on the soil are the primary cause of weed infestations in arable fields. Previous studies have documented reductions in weed seedbanks due to cropping system diversification through extended rotation sequences, but the impacts of different rotation systems on additions to and losses from weed seedbanks remain poorly understood. We conducted an experiment in Iowa, USA, to determine the fates of Setaria faberi and Abutilon theophrasti seeds in 2-, 3- and 4-year crop rotation systems when seed additions to the soil seedbank were restricted to a single pulse at the initiation of the study. Over the course of the experiment, seedlings were removed as they emerged and prevented from producing new seeds. After 41months, seed population densities dropped >85% for S.faberi and >65% for A.theophrasti, but differences between rotation systems in the magnitude of seedbank reductions were not detected. Most of the reductions in seedbank densities took place from autumn through early spring in the first 5months following seed deposition, before seedling emergence occurred, suggesting that seed predation and/or seed decay was important. For S.faberi, total cumulative seedling emergence and total seed mortality did not differ between rotation systems. In contrast, for A.theophrasti, seedling emergence was 71% lower and seed mortality was 83% greater in the 3- and 4-year rotation systems than in the 2-year system. Results of this study indicate that for certain weed species, such as A.theophrasti, crop rotation systems can strongly affect life-history processes associated with soil seedbanks. C1 [Liebman, M.; Sundberg, D. N.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Miller, Z. J.; Menalled, F. D.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Williams, C. L.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Westerman, P. R.] Univ Rostock, Grp Crop Hlth, D-18055 Rostock, Germany. [Dixon, P. M.] Iowa State Univ, Dept Stat, Ames, IA USA. [Heggenstaller, A.] Dupont Pioneer, Johnston, IA USA. [Davis, A. S.] ARS, USDA, Urbana, IL USA. RP Liebman, M (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM mliebman@iastate.edu FU USDA National Research Initiative [2002-35320-12175, 2006-35320-16548]; Leopold Center for Sustainable Agriculture [2004-E6] FX We extend our sincere thanks to A. Anderson, J. Borza, M. Burns, R. Donahoo, D. Farnsworth, M. Fiscus, D. Franzenburg, G. Fuerst, R. Graef, F. Graziani, R. Hartzler, S. Kudron, T. Lux, G. McAndrews, A. Messner, L. Miller, M. O'Rourke, D. Rosmann, A. Schwarte, D. Thompson, D. Williams and A. Wright for their assistance. We also thank the subject editor for particularly insightful comments and suggestions. Funding for this work was provided by the USDA National Research Initiative (Projects 2002-35320-12175 and 2006-35320-16548) and the Leopold Center for Sustainable Agriculture (Project 2004-E6). NR 31 TC 3 Z9 3 U1 4 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD JUN PY 2014 VL 54 IS 3 BP 293 EP 306 DI 10.1111/wre.12069 PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AF3CH UT WOS:000334588600009 ER PT J AU Lv, JY Rao, JP Zhu, YM Chang, XX Hou, YL Zhu, QG AF Lv, Jingyi Rao, Jingping Zhu, Yanmin Chang, Xiaoxiao Hou, Yali Zhu, Qinggang TI Cloning and expression of lipoxygenase genes and enzyme activity in ripening persimmon fruit in response to GA and ABA treatments SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Lipoxygenases; Gene expression; qRT-PCR; Ethylene; ABA treatment; GA(3) treatment ID TRIGGERING ETHYLENE BIOSYNTHESIS; CELL-WALL METABOLISM; GRANNY SMITH APPLES; ABSCISIC-ACID; TOMATO FRUIT; 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID; CLIMACTERIC FRUITS; GIBBERELLIC-ACID; STRAWBERRY FRUIT; DIFFERENTIAL EXPRESSION AB Two genes of the lipoxygenase (LOX) family, DkLox1 and DkLox3 (GenBank accession No. JF436951 and JF436950), were cloned from persimmon fruit (Diospyros kaki L. 'Fuping Jianshi'). Sequence analysis indicated that they belong to the 9-LOX sub-group. Transcriptional profiles of both LOX genes during fruit ripening and in response to gibberellin (GA(3)) and abscisic acid (ABA) treatments during postharvest storage were studied using quantitative reverse transcription PCR (qRT-PCR). The expression of DkLox1 and DkLox3 peaked at 15 d after the fruit were harvested under room temperature, corresponding to the patterns of LOX activity and ethylene production. ABA treatment accelerated the persimmon fruit softening and increased LOX enzyme activity, ethylene production and MDA content; and the expression of DkLox1 and DkLox3 peaked three days earlier than the non-treated control. By contrast, the GA3 treatment decelerated the ripening process and down-regulated the expression of DkLox1 and DkLox3 genes. The roles of LOX gene expression during persimmon fruit ripening are discussed. Published by Elsevier B.V. C1 [Lv, Jingyi; Rao, Jingping; Chang, Xiaoxiao; Hou, Yali; Zhu, Qinggang] Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [Zhu, Yanmin] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Rao, JP (reprint author), Northwest A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. EM jingping.rao@hotmail.com; yanmin.zhu@ars.usda.gov FU National Natural Science Foundation of China [30771756] FX This work was supported by the National Natural Science Foundation of China (30771756). The authors wish to thank Sungbong Shin, Franklin Johnson, Kate Evans, Tianbao Yang and Steve Reymond for their critical manuscript reviewing. NR 67 TC 9 Z9 10 U1 8 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD JUN PY 2014 VL 92 BP 54 EP 61 DI 10.1016/j.postharvbio.2014.01.015 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AE6IV UT WOS:000334095100008 ER PT J AU Haider, N Rahman, MS Khan, SU Mikolon, A Gurley, ES Osmani, MG Shanta, IS Paul, SK Macfarlane-Berry, L Islam, A Desmond, J Epstein, JH Daszak, P Azim, T Luby, SP Zeidner, N Rahman, MZ AF Haider, N. Rahman, M. S. Khan, S. U. Mikolon, A. Gurley, E. S. Osmani, M. G. Shanta, I. S. Paul, S. K. Macfarlane-Berry, L. Islam, A. Desmond, J. Epstein, J. H. Daszak, P. Azim, T. Luby, S. P. Zeidner, N. Rahman, M. Z. TI Identification and Epidemiology of a Rare HoBi-Like Pestivirus Strain in Bangladesh SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE BVDV-3; HoBi-like pestivirus; Bangladesh; cattle ID VIRAL DIARRHEA VIRUS; INFECTION; SUBSTITUTIONS; CATTLE AB The genus pestivirus of the family flaviviridae consists of four recognized species: bovine viral diarrhoea virus 1 (BVDV-1), bovine viral diarrhoea virus 2 (BVDV-2), classical swine fever virus and border disease virus. A new putative pestivirus species tentatively named as either 'HoBi-like pestivirus' or BVDV-3 has recently been identified in Brazil, Italy and Thailand. Despite reports of serological evidence of BVDV in Bangladesh, the types of the virus circulating in cattle have not been identified. We conducted surveillance in cattle from May 2009 to August 2010 in three government veterinary hospitals to characterize BVDV in cattle of Bangladesh. We tested serum for BVDV using an antigen-capture ELISA. Of 638 cattle samples, 3% (16/638) tested positive for BVDV antigen. The ELISA-positive samples were selected for further molecular detection and characterization of BVDV. Molecular analysis of the partial 5 ' untranslated region (UTR) nucleotide sequences of BVDV-positive samples identified the rare HoBi-like pestivirus or BVDV-3 virus circulating in cattle of Bangladesh. The identification of this rare HoBi-like pestivirus or BVDV-3 strain in Bangladesh warrants further surveillance to evaluate its impact on livestock production. C1 [Haider, N.; Rahman, M. S.; Khan, S. U.; Gurley, E. S.; Shanta, I. S.; Paul, S. K.; Macfarlane-Berry, L.; Azim, T.; Luby, S. P.; Zeidner, N.; Rahman, M. Z.] Int Ctr Diarrheal Dis Res, Dhaka, Bangladesh. [Khan, S. U.] Univ Florida, Coll Publ Hlth & Hlth Profess, Gainesville, FL USA. [Mikolon, A.] USDA, Hawthorne, CA USA. [Osmani, M. G.] Minist Fisheries, Dept Livestock Serv, Dhaka, Bangladesh. [Osmani, M. G.] Minist Livestock, Dept Livestock Serv, Dhaka, Bangladesh. [Islam, A.; Desmond, J.; Epstein, J. H.; Daszak, P.] EcoHlth Alliance, New York, NY USA. [Luby, S. P.] Stanford Univ, Stanford, CA 94305 USA. [Zeidner, N.] US Ctr Dis Control & Prevent, Atlanta, GA USA. RP Rahman, MZ (reprint author), Icddr B, Ctr Communicable Dis, Virol Lab, 68 Shaheed Tajuddin Ahmed Sarani, Dhaka 1212, Bangladesh. EM mzrahman@icddrb.org RI Haider, Najmul/F-6083-2015; Gurley, Emily/B-7903-2010; OI Haider, Najmul/0000-0002-5980-3460; Gurley, Emily/0000-0002-8648-9403; Luby, Stephen/0000-0001-5385-899X FU Google; Rockefeller foundation through EcoHealth Alliance FX This research was funded by Google and the Rockefeller foundation through EcoHealth Alliance. icddr,b acknowledges with gratitude the commitment of Google and the Rockefeller foundation to its research efforts. We acknowledge the Department of Livestock Services (DLS) of Bangladesh and the veterinarians Dr. Asma-Al-Hoseneara and Dr. Rashidul Hoque and Dr. Shama Ranjan Barua from DLS, Dr. Abdul Mannan and Dr. Shahneaz Ali Khan, from Chittagong Veterinary and Animal Sciences University, and Tahmina Sultana from icddr, b who were involved with this project. We are grateful to Ms. Diana DiazGranados, icddr, b, for her constructive support in editing this manuscript. NR 25 TC 12 Z9 15 U1 0 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2014 VL 61 IS 3 BP 193 EP 198 DI 10.1111/tbed.12218 PG 6 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA AE9BH UT WOS:000334298300001 PM 24650238 ER PT J AU Palmer, MV Thacker, TC Waters, WR Robbe-Austerman, S Aldwell, FE AF Palmer, M. V. Thacker, T. C. Waters, W. R. Robbe-Austerman, S. Aldwell, F. E. TI Persistence of Mycobacterium bovis bacillus Calmette-Guerin (BCG) Danish In White-tailed Deer (Odocoileus virginianus) Vaccinated with a Lipid-Formulated Oral Vaccine SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE Odocoileus; BCG; Mycobacterium bovis; vaccination; oral; lipid; persistence; deer ID POSSUMS TRICHOSURUS-VULPECULA; BRUSHTAIL POSSUMS; PULMONARY TUBERCULOSIS; IMMUNE-RESPONSES; NEW-ZEALAND; IN-VIVO; MICHIGAN; INFECTION; WILDLIFE; MICE AB Mycobacterium bovis, the causative agent of tuberculosis in animals, has a broad host range, including humans. Historically, public health concerns prompted programs to eradicate tuberculosis from cattle in many nations. Eradication efforts decreased the prevalence of bovine tuberculosis; nevertheless, some countries encountered significant obstacles, not least of which was a wildlife reservoir of M. bovis. Efforts to decrease the size of the affected wildlife populations have neither eliminated disease nor eliminated transmission to cattle. Consequently, the use of a vaccine for wildlife is being explored. The vaccine most studied is M. bovis BCG, an attenuated live vaccine, first developed 100years ago. The most efficient and effective means of vaccinating wildlife will be an oral vaccine. White-tailed deer in Michigan, USA, constitute a reservoir of M. bovis. White-tailed deer are a popular game species, and as such, represent a food animal to many hunters. BCG persistence in deer tissues could result in human exposure to BCG. Although non-pathogenic, BCG exposure could induce false-positive skin test results, confounding the central component of public health surveillance for TB. The objective of the present study in white-tailed deer was to evaluate persistence of lipid-encapsulated BCG and a liquid suspension of BCG after oral administration at two different dosages. Vaccine was not recovered at any time after oral consumption of a bait containing a single dose (1x10(8) CFU) of lipid-encapsulated BCG. However, persistence was consistent in deer consuming 10 lipid-encapsulated baits (1x10(9) CFU), with BCG recovered from at least one deer at 1, 3, 6, 9 and 12months after consumption. Persistence of up to 9months was seen in deer vaccinated with orally with a liquid suspension. Persistence of BCG was limited to lymphoid tissue and never found in samples of muscle collected at each time point. Although the risk of exposure to hunters is low, BCG persistence should be considered prior to field use in white-tailed deer. C1 [Palmer, M. V.; Thacker, T. C.; Waters, W. R.] ARS, Infect Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Robbe-Austerman, S.] USDA, Natl Vet Serv Labs, Anim & Plant Hlth Inspect Serv, Ames, IA 50010 USA. [Aldwell, F. E.] Univ Otago, Immune Solut Ltd, Dunedin, New Zealand. RP Palmer, MV (reprint author), Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM Mitchell.Palmer@ars.usda.gov OI Thacker, Tyler/0000-0001-6779-7649 NR 46 TC 11 Z9 11 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD JUN PY 2014 VL 61 IS 3 BP 266 EP 272 DI 10.1111/tbed.12032 PG 7 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA AE9BH UT WOS:000334298300008 PM 23173832 ER PT J AU Collins, BM AF Collins, Brandon M. TI Fire weather and large fire potential in the northern Sierra Nevada SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Wildfire; Fire management; Climate change ID WESTERN UNITED-STATES; MIXED-CONIFER FOREST; CLIMATE-CHANGE; WILDFIRE; CALIFORNIA; USA; SEVERITY; FUELS; BEHAVIOR; OSCILLATION AB Fuels, weather, and topography all contribute to observed fire behavior. Of these, weather is not only the most dynamic factor, it is the most likely to be directly influenced by climate change. In this study 40 years of daily fire weather observations from five weather stations across the northern Sierra Nevada were analyzed to investigate potential changes or trends in the frequency of high- to extreme-fire weather. The analysis demonstrated fairly strong upward trends in the occurrence of high- to extreme-fire weather, particularly since the mid-1990s. This increased occurrence of high fire weather conditions suggests that there is more opportunity for fires to grow rapidly and overwhelm initial suppression efforts, likely resulting in greater incidence of large fires throughout the region. This is particularly problematic in the northern Sierra Nevada, where there is a complex arrangement of land ownerships, including numerous human communities. Published by Elsevier B.V. C1 [Collins, Brandon M.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. RP Collins, BM (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. EM bcollins@berkeley.edu FU USFS Pacific Southwest Research Station FX Jim Baldwin provided statistical expertise and helped develop specific statistical analyses. Gary Roller helped with initial data acquisition. Scott Stephens and Eric Knapp also provided helpful comments that improved the manuscript. Funding was provided by the USFS Pacific Southwest Research Station. NR 51 TC 17 Z9 17 U1 5 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 1 PY 2014 VL 189 BP 30 EP 35 DI 10.1016/j.agrformet.2014.01.005 PG 6 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AE3BW UT WOS:000333852900005 ER PT J AU Shapland, TM Snyder, RL U, KTP McElrone, AJ AF Shapland, T. M. Snyder, R. L. U, K. T. Paw McElrone, A. J. TI Thermocouple frequency response compensation leads to convergence of the surface renewal alpha calibration SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Coherent structures; Response time; Sensible heat flux density; Spectral attenuation; Structure function; Temperature ramps ID SENSIBLE HEAT-FLUX; TEMPERATURE STRUCTURE FUNCTIONS; 3 CONTRASTING SURFACES; BOUNDARY-LAYER; COHERENT EDDIES; PART II; CONVECTION; ENERGY; SIMILARITY; DENSITY AB Ramp-like shapes in the turbulent scalar trace are the signature of coherent structures, and their characteristics (i.e., amplitude and duration) are resolved via a structure-function model for use in the surface renewal flux calculation. The potential for surface renewal to provide inexpensive sensible heat flux measurements has not been fully realized because this method has required calibration against eddy covariance or other more expensive flux measurement techniques. The calibration factor alpha is ideally 0.5, but a broad range of values have been reported in the surface renewal literature. Although it has been hypothesized that the sensor size, and hence sensor frequency response characteristics, influence alpha, no effort has been previously made to compensate the thermocouple signal in surface renewal measurements. We evaluate methods for compensating the frequency response of a thermocouple in the time domain and the frequency domain, and we present a novel method for compensation in the lag domain (i.e., compensating the structure function directly). We evaluated the compensation procedure as it affects the resolution of ramp characteristics at both the smallest and the second smallest scales of ramp-like turbulent shapes. The surface renewal sensible heat flux estimates from the compensated robust thermocouples (76 pm diameter wire) agree well with the estimates from the compensated fragile thermocouples (13 pm diameter). Using both the data collected for the present experiment and a meta-analysis of data in the surface renewal literature, we correct the surface renewal estimates for thermocouple frequency response characteristics to obtain alpha calibrations that converge to close to the predicted value of 0.5. We conclude that the frequency response characteristics of the thermocouple are the prevailing influence on the alpha calibrations reported in the literature. Published by Elsevier B.V. C1 [Shapland, T. M.; McElrone, A. J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Snyder, R. L.; U, K. T. Paw] Univ Calif Davis, Davis, CA 95616 USA. [McElrone, A. J.] ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP McElrone, AJ (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM tmshapland@ucdavis.edu; rlsnyder@ucdavis.edu; ktpawu@ucdavis.edu; ajmcelrone@ucdavis.edu FU National Grape and Wine Institute; NIFA Specialty Crops Research Initiative grant; USDA-ARS CRIS [5306-21220-004-00]; J. Lohr Vineyards Wines FX Partial support for this research was provided by J. Lohr Vineyards & Wines, the National Grape and Wine Institute, a NIFA Specialty Crops Research Initiative grant, and USDA-ARS CRIS funding (Research Project #5306-21220-004-00). The authors thank Mike Mata and Eric Kent for assistance with the field experiments, and Sam Matoba for assistance with the stage micrometer. NR 44 TC 3 Z9 4 U1 0 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 1 PY 2014 VL 189 BP 36 EP 47 DI 10.1016/j.agrformet.2014.01.008 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AE3BW UT WOS:000333852900006 ER PT J AU Canfield, JM Linn, RR Sauer, JA Finney, M Forthofer, J AF Canfield, J. M. Linn, R. R. Sauer, J. A. Finney, M. Forthofer, Jason TI A numerical investigation of the interplay between fireline length, geometry, and rate of spread SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Fire; Fireline; Wildfire; Spread; Vorticity; Vortex ID DISCRETE FUEL-ELEMENTS; MODELING FOREST-FIRES; WIND-AIDED FIRESPREAD; POISEUILLE FLOW; BED; PROPAGATION; CONVECTION; GRASSLANDS; ARRAYS; PLATES AB The current study focuses on coupled dynamics and resultant geometry of fireline segments of various ignition lengths. As an example, for ignition lines of length scales typical for field experiments, fireline curvature is the result of a competition between the head fire and the flanks of the fire. A number of physical features (i.e. buoyancy and wind field divergence for example) arise in and around an incipient fire that defines the shape and spreading pattern of the flame zone. These features are explored using a numerical atmospheric dynamics model HIGRAD, and wildfire combustion physics model FIRETEC. HIGRAD/FIRETEC was designed to investigate wildfires and their interactions with the environment. In this study, the model was used to simulate grass fires that were initiated with a finite length, straight ignition line in homogeneous fuels. The dynamic evolutions of these firelines were analyzed to understand the individual events that evolve a wildfire. By understanding each individual process and how it interacts with other processes, information can be extracted to develop a theory about the mechanisms that combine to produce the observed wildfire behavior. In the current study, the flow field in the region of the simulated fires developed structures consistent with multiple buoyancy-induced vortex pairs. The series of stream-wise vortex pairs produce a regular alternating pattern of up-wash and down-wash zones, which allow air to penetrate the flame zone through troughs created in downwash regions. Consequently, this periodicity in the flow field within the fire resulted in a pattern of residual combustion where prolonged burning occurred in the up-wash zones separated by near-complete fuel depletion in the downwash zones. Some explanation is provided for why increased ignition line length leads to increased rate of spread (ROS) with some asymptotic limit. Published by Elsevier B.V. C1 [Canfield, J. M.; Linn, R. R.; Sauer, J. A.] Los Alamos Natl Lab, Div Earth & Environm Sci, Los Alamos, NM 87545 USA. [Finney, M.; Forthofer, Jason] US Forest Serv, Fire Lab, USDA, Missoula, MT 59808 USA. RP Canfield, JM (reprint author), Los Alamos Natl Lab, Div Earth & Environm Sci, Los Alamos, NM 87545 USA. EM jessec@lanl.gov FU Rocky Mountain Research Station, USDA Forest Service; LANL ICN; Kitware FX We thank Kevin Heirs and the Joseph W. Jones Ecological Research Center at Ichauway in addition to Caroline Sieg and the Rocky Mountain Research Station, USDA Forest Service for funding this research via resources from the National Fire Plan. All computations were performed through the Los Alamos National Laboratory Institutional Computing Center (LANL ICN). Simulated fire visualizations were created using ParaView visualization software, maintained by Kitware. Many thanks go to LANL ICN and Kitware for the resources and support provided for this work. We also thank Philip Cunningham for many valuable discussions into the details of vorticity and the role that it plays in wildfire behavior. NR 42 TC 1 Z9 1 U1 3 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD JUN 1 PY 2014 VL 189 BP 48 EP 59 DI 10.1016/j.agrformet.2014.01.007 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AE3BW UT WOS:000333852900007 ER PT J AU Rohrer, GA Rempel, LA Miles, JR Keele, JW Wiedmann, RT Vallet, JL AF Rohrer, G. A. Rempel, L. A. Miles, J. R. Keele, J. W. Wiedmann, R. T. Vallet, J. L. TI Identifying genetic loci controlling neonatal passive transfer of immunity using a hybrid genotyping strategy SO ANIMAL GENETICS LA English DT Article DE pooled DNA; immunoglobulin; GWAS; pig ID GENOME-WIDE ASSOCIATION; POOLED DNA; CANDIDATE MARKERS; NEWBORN CALVES; LITTER SIZE; FOOD-INTAKE; IDENTIFICATION; POLYMORPHISM; CATTLE; HAPLOTYPES AB Colostrum intake is critical to a piglet's survival and can be measured by precipitating out the gamma-immunoglobulins from serum with ammonium sulfate (immunocrit). Genetic analysis of immunocrits on 5312 piglets indicated that the heritabilities (se) for direct and maternal effects were 0.13 (0.06) and 0.53 (0.08) respectively. To identify QTL for direct genetic effects, piglets with the highest and lowest immunocrits from 470litters were selected. Six sets of DNA pools were created based on sire of the litter. These 12 DNA pools were applied to Illumina Porcine SNP60 BeadChips. Normalized X and Y values were analyzed. Three different SNP selection methods were used: deviation of the mean from high vs. low pools, the deviation adjusted for variance based on binomial theory and ANOVA. The 25 highest ranking SNPs were selected from each evaluation for further study along with 12 regions selected based on a five-SNP window approach. Selected SNPs were individually genotyped in the 988 piglets included in pools as well as in 524 piglets that had intermediate immunocrits. Association analyses were conducted fitting an animal model using the estimated genetic parameters. Nineteen SNPs were nominally associated (P<0.01) with immunocrit values, of which nine remained significant (P<0.05) after Bonferroni correction, located in 16 genomic regions on 13 chromosomes. In conclusion, the pooling strategy reduced the cost to scan the genome by more than 80% and identified genomic regions associated with a piglet's ability to acquire gamma-immunoglobulin from colostrum. Each method to rank SNPs from the pooled analyses contributed unique validated markers, suggesting that multiple analyses will reveal more QTL than a single analysis. C1 [Rohrer, G. A.; Rempel, L. A.; Miles, J. R.; Keele, J. W.; Wiedmann, R. T.; Vallet, J. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Rohrer, GA (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM gary.rohrer@ars.usda.gov FU CRIS from the Agricultural Research Service, U.S. Department of Agriculture (USDA) [5438-31000-083-00D] FX The authors would like to acknowledge the expert technical assistance of Mr. Mike Judy for immunocrit data, Ms. Kris Simmerman for genotypic data collection and Ms. Linda Parnell for manuscript preparation. This work was funded by CRIS #5438-31000-083-00D from the Agricultural Research Service, U.S. Department of Agriculture (USDA). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program (Not all prohibited bases apply to all programs). Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 202509410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 31 TC 1 Z9 1 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD JUN PY 2014 VL 45 IS 3 BP 340 EP 349 DI 10.1111/age.12131 PG 10 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AE4ZN UT WOS:000333997700004 PM 24779640 ER PT J AU Sharma, BK Moser, BR Vermillion, KE Doll, KM Rajagopalan, N AF Sharma, Brajendra K. Moser, Bryan R. Vermillion, Karl E. Doll, Kenneth M. Rajagopalan, Nandakishore TI Production, characterization and fuel properties of alternative diesel fuel from pyrolysis of waste plastic grocery bags SO FUEL PROCESSING TECHNOLOGY LA English DT Article DE Plastic; Pyrolysis; High-density polyethylene; Fuel; Diesel; Biodiesel ID MARINE DEBRIS; BIO-OIL; HYDROTHERMAL LIQUEFACTION; PETROLEUM DIESEL; SURFACE-TENSION; BED REACTOR; SEA-TURTLES; BIODIESEL; BLENDS; DEGRADATION AB Pyrolysis of HDPE waste grocery bags followed by distillation resulted in a liquid hydrocarbon mixture with average structure consisting of saturated aliphatic paraffinic hydrogens (96.8%), aliphatic olefinic hydrogens (2.6%) and aromatic hydrogens (0.6%) that corresponded to the boiling range of conventional petroleum diesel fuel (#1 diesel 190-290 degrees C and #2 diesel 290-340 degrees C). Characterization of the liquid hydrocarbon mixture was accomplished with gas chromatography-mass spectroscopy, infrared and nuclear magnetic resonance spectroscopies, size exclusion chromatography, and simulated distillation. No oxygenated species such as carboxylic acids, aldehydes, ethers, ketones, or alcohols were detected. Comparison of the fuel properties to the petrodiesel fuel standards ASTM D975 and EN 590 revealed that the synthetic product was within all specifications after addition of antioxidants with the exception of density (802 kg/m(3)). Notably, the derived cetane number (73.4) and lubricity (198 mu m, 60 degrees C, ASTM D6890) represented significant enhancements over those of conventional petroleum diesel fuel. Other fuel properties included a kinematic viscosity (40 degrees C) of 2.96 mm(2)/s, cloud point of 4.7 degrees C. flash point of 81.5 degrees C, and energy content of 46.16 MJ/kg. In summary, liquid hydrocarbons with appropriate boiling range produced from pyrolysis of waste plastic appear suitable as blend components for conventional petroleum diesel fuel. (C) 2014 Elsevier B.V. All rights reserved. C1 [Sharma, Brajendra K.; Rajagopalan, Nandakishore] Univ Illinois, Prairie Res Inst, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Moser, Bryan R.; Vermillion, Karl E.; Doll, Kenneth M.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Sharma, BK (reprint author), Univ Illinois, Prairie Res Inst, Illinois Sustainable Technol Ctr, 1 Hazelwood Dr, Champaign, IL 61820 USA. EM bksharma@illinois.edu FU Illinois Hazardous Waste Research Fund FX The authors acknowledge Dheeptha Murali (ISTC), Jennifer L Deluhery (ISTC), Benetria N. Banks (USDA-ARS-NCAUR) and Erin L Walter (USDA-ARS-NCAUR) for excellent technical assistance. This study was supported in part by seed funding from the Illinois Hazardous Waste Research Fund. NR 50 TC 25 Z9 25 U1 14 U2 93 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3820 EI 1873-7188 J9 FUEL PROCESS TECHNOL JI Fuel Process. Technol. PD JUN PY 2014 VL 122 BP 79 EP 90 DI 10.1016/j.fuproc.2014.01.019 PG 12 WC Chemistry, Applied; Energy & Fuels; Engineering, Chemical SC Chemistry; Energy & Fuels; Engineering GA AE5AH UT WOS:000333999700010 ER PT J AU Lee, WH Kim, MS Lee, H Delwiche, SR Bae, H Kim, DY Cho, BK AF Lee, Wang-Hee Kim, Moon S. Lee, Hoonsoo Delwiche, Stephen R. Bae, Hanhong Kim, Dae-Yong Cho, Byoung-Kwan TI Hyperspectral near-infrared imaging for the detection of physical damages of pear SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Hyperspectral imaging; F-value classification algorithm; Image processing; Near infrared spectrum; Pear bruise ID BRUISE DETECTION; APPLES; SPECTROSCOPY AB Bruise damage on pears is one of the most crucial internal quality factors, which needs to be detected in postharvest quality sorting processes. Near-infrared imaging techniques (NIR) have effective potentials for identifying and detecting bruises since bruises result in the rupture of internal cell walls due to defects on agricultural materials. In this study, a novel NIR technique, hyperspectral imaging with beyond NIR range of 950-1650 nm, was investigated for detecting bruise damages underneath the pear skin, which has never been examined in the past. A classification algorithm based on F-value was applied for analysis of image to find the optimal waveband ratio for the discrimination of bruises against sound surface. The result demonstrated that the best threshold waveband ratio detected bruises with the accuracy of 92%, illustrating that the hyperspectral infra-red imaging technique with the region beyond NIR could be a potential detection method for pear bruises. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Lee, Wang-Hee; Lee, Hoonsoo; Kim, Dae-Yong; Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Taejon 305764, South Korea. [Kim, Moon S.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Delwiche, Stephen R.] ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. [Bae, Hanhong] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea. RP Cho, BK (reprint author), 99 Deahak Ro St, Taejon 305764, South Korea. EM chobk@cnu.ac.kr FU Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs(MAFRA), Republic of Korea; Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center), Rural Development Administration, Republic of Korea [PJ009078] FX This work was partially supported by the Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs(MAFRA), Republic of Korea. It was also partially supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ009078), Rural Development Administration, Republic of Korea. NR 22 TC 24 Z9 29 U1 1 U2 38 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD JUN PY 2014 VL 130 BP 1 EP 7 DI 10.1016/j.jfoodeng.2013.12.032 PG 7 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA AE5BB UT WOS:000334001700001 ER PT J AU Gent, DH Grove, GG Nelson, ME Wolfenbarger, SN Woods, JL AF Gent, D. H. Grove, G. G. Nelson, M. E. Wolfenbarger, S. N. Woods, J. L. TI Crop damage caused by powdery mildew on hop and its relationship to late season management SO PLANT PATHOLOGY LA English DT Article DE quantitative epidemiology; crop loss; Humulus lupulus ID WINTER-WHEAT; DOWNY MILDEW; YIELD; SEVERITY; DISEASE; GROWTH AB Powdery mildew of hop (Podosphaera macularis) may cause economic loss due to reductions in cone yield and quality. Quantitative estimates of crop damage from powdery mildew remain poorly characterized, especially the effect of late season disease management on crop yield and quality. Field studies in Washington State evaluated cone yield, bittering acid content and quality factors when fungicide applications were ceased at different stages of cone development. The incidence of cones with powdery mildew was linearly correlated with yield of cones, bittering acids and accelerated cone maturation. In cultivar Galena, the cumulative effect of every 1% increase in cones powdery mildew incidence was to reduce alpha-acid yield by 0 center dot 33%, which was due to direct effects on cone yield but also indirect effects mediated by dry matter. In the more susceptible cultivar Zeus, alpha-acid yield was increased 20% by controlling powdery mildew through the transition of bloom to early cone development compared to ceasing fungicide applications at bloom: additional applications provided only modest improvements in alpha-acid yield. In both cultivars, the impact of powdery mildew on aroma characteristics and bittering acid content were less substantial than cone yield. The damage caused by powdery mildew to cone colour and alpha-acid yield, as well as the effectiveness of fungicide applications made to manage the disease, appears inseparably linked to dry matter content of cones at harvest. Realising achievable yield potential in these cultivars requires control of the disease through early stages of cone development and harvest before maturity exceeds c. 25% dry matter. C1 [Gent, D. H.] USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D. H.; Nelson, M. E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Grove, G. G.; Wolfenbarger, S. N.] Washington State Univ, Dept Plant Pathol, Irrigated Agr Res & Extens Ctr, Prosser, WA 99164 USA. [Woods, J. L.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. RP Gent, DH (reprint author), USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU USDA-ARS CRIS [5358-21000-045-00]; Washington State University; Hop Research Council; Washington State Commission on Pesticide Registrations; Western IPM Center; USDA Specialty Crops Research Initiative FX Financial support was provided by USDA-ARS CRIS 5358-21000-045-00, Washington State University, the Hop Research Council, Washington State Commission on Pesticide Registrations, Western IPM Center, and USDA Specialty Crops Research Initiative. The authors thank Walt Mahaffee, Megan Twomey and the reviewers for their comments that improved this paper, and are indebted to their technical support staff for the work that made this research possible. Disclaimer: The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 36 TC 6 Z9 6 U1 2 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD JUN PY 2014 VL 63 IS 3 BP 625 EP 639 DI 10.1111/ppa.12123 PG 15 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AE4YX UT WOS:000333995900015 ER PT J AU Follett, PA Rivera-Leong, K Myers, R AF Follett, Peter A. Rivera-Leong, Keao Myers, Roxana TI Rice weevil response to basil oil fumigation SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Sitophilus oryzae; Plant essential oil; Quarantine treatment; Ocimum basilicum; Rice; Pest management ID COLEOPTERA-CURCULIONIDAE AB Basil oil, Ocimum basilicum L, is a volatile plant essential oil that is known to have insecticidal activity against stored product pests such as rice weevil, Sitophilus oryzae (L). Basil oil was diluted in acetone and applied to a sponge held inside a tea strainer for fumigations in containers with and without rice. Basil oil fumigation (3 ml, 10% concentration) caused high mortality in adult rice weevils when weevils were exposed in air in a sealed 1 L plastic container. However, when basil oil was placed in packaged rice, weevil mortality was low and reproduction was not affected. Effectiveness of plant essential oil fumigation should be conducted under realistic conditions to avoid experimental artifacts and misleading results. Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. C1 [Follett, Peter A.; Rivera-Leong, Keao; Myers, Roxana] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Follett, PA (reprint author), USDA ARS, 64 Nowelo St, Hilo, HI 96720 USA. EM Peter.follett@ars.usda.gov NR 16 TC 1 Z9 2 U1 0 U2 21 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD JUN PY 2014 VL 17 IS 2 BP 119 EP 121 DI 10.1016/j.aspen.2013.11.008 PG 3 WC Entomology SC Entomology GA AE3IF UT WOS:000333869500002 ER PT J AU Wu, BG Pan, ZL Qu, WJ Wang, B Wang, J Ma, HL AF Wu, Bengang Pan, Zhongli Qu, Wenjuan Wang, Bei Wang, Juan Ma, Haile TI Effect of simultaneous infrared dry-blanching and dehydration on quality characteristics of carrot slices SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Infrared; Blanching; Dehydration; Carrot; Model ID THERMAL INACTIVATION; KINETIC-PARAMETERS; ENZYME-ACTIVITY; APPLE SLICES; PEROXIDASE; RADIATION; BLUEBERRIES; MICROWAVE; COLOR; LIPOXYGENASE AB This study investigated the effects of various processing parameters on carrot slices exposed to infrared (IR) radiation heating for achieving simultaneous infrared dry-blanching and dehydration (SIRDBD). The investigated parameters were product surface temperature, slice thickness and processing time. A three-factor factorial design was conducted to determine the influence of processing parameters on moisture reduction, drying rate, residual peroxidase (POD) activities, surface color change and vitamin C retention. High surface temperature and/or thin slices had faster inactivation of enzymes and quicker moisture removal compared to the low surface temperature and/or thick slices. A Middili model performed well for describing drying behavior during the treatment, while a biphasic model and a quantic model fit well for describing the POD inactivation and the surface color change during the process, respectively. The process which produced 1 log reduction in POD activity has resulted in moisture reduction from 40.2 to 88.8 g/100 g, overall color change (Delta E) from 3.17 to 5.13 and retention of vitamin C from 56.92 to 77.34 g/100 g compared to control. It was concluded that SIRDBD could be used as an alternative to produce high quality blanched and partially dehydrated fruits and vegetables. Published by Elsevier Ltd. C1 [Wu, Bengang; Pan, Zhongli; Qu, Wenjuan; Wang, Bei; Wang, Juan; Ma, Haile] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China. [Pan, Zhongli] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP Pan, ZL (reprint author), ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM zhongli.pan@ars.usda.gov; mhl@ujs.edu.cn FU Scientific Innovation Research of College Graduate in Jangsu Province [CXZZ12_0700]; Science and Technology Support Program - Agriculture of Jiangsu Province [BE2011382]; Science and Technology of Jiangsu Province International Cooperation [SEZ201200380]; Science and Technology Assistance to Xinjiang [2013AB019]; Open Fund of Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products [JAPP2010-6]; Zhenjiang Maybo Innovation Co., Ltd. FX The authors wish to thank for the supports received from program (CXZZ12_0700) of Scientific Innovation Research of College Graduate in Jangsu Province, grant (BE2011382) of the Science and Technology Support Program - Agriculture of Jiangsu Province, program (SEZ201200380) of the Science and Technology of Jiangsu Province International Cooperation, program (2013AB019) of the Science and Technology Assistance to Xinjiang, program (JAPP2010-6) of the Open Fund of Jiangsu Provincial Key Laboratory for Physical Processing of Agricultural Products, and Zhenjiang Maybo Innovation Co., Ltd. NR 51 TC 15 Z9 21 U1 5 U2 56 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUN PY 2014 VL 57 IS 1 BP 90 EP 98 DI 10.1016/j.lwt.2013.11.035 PG 9 WC Food Science & Technology SC Food Science & Technology GA AD7XA UT WOS:000333479600012 ER PT J AU Hsu, H Sheen, S Sites, J Huang, LH Wu, JSB AF Hsu, HsinYun Sheen, Shiowshuh Sites, Joseph Huang, Lihan Wu, James Swi-Bea TI Effect of high pressure treatment on the survival of Shiga toxin-producing Escherichia coli in strawberry puree SO FOOD MICROBIOLOGY LA English DT Article DE Shiga toxin-producing Escherichia coli; Strawberry puree; High pressure processing ID HIGH HYDROSTATIC-PRESSURE; LISTERIA-MONOCYTOGENES; MICROBIAL SAFETY; UNITED-STATES; INACTIVATION; SALMONELLA; JUICE; MICROORGANISMS; DEGRADATION; O157-H7 AB Most fresh produce, such as strawberries, receives minimal processing and is often eaten raw. Contamination of produce with pathogenic bacteria may occur during growth, harvest, processing, transportation, and storage (abuse temperature) and presents a serious public health risk. Strawberries have been implicated in an outbreak of Escherichia coli O157:H7 infection that sickened 15 people, including one death. Strawberries may also be contaminated by other serogroups of non-O157 Shiga toxin-producing E. coli (STEC), including O26, O45, O103, O111, O121 and O145, which have become known as the "Big Six" or "Top Six" non-O157 STECs. The objective of this research was to explore the potential application of high pressure processing (HPP) treatment to reduce or eliminate STECs in fresh strawberry puree (FSP). FSP, inoculated with a six-strain cocktail of the "Big Six" non-O157 STEC strains or a five-strain cocktail of E. coli O157:H7 in vacuum-sealed packages, were pressure-treated at 150, 250, 350, 450, 550, and 650 MPa (1 MPa = 10(6) N/m(2)) for 5, 15, and 30 min. HPP treatment, at 350 MPa for >= 5 min, significantly reduced STECs in FSP by about 6-log CFU/g from the initial cell population of ca. 8-log CFU/g. Cell rupture, observed by scanning electron microscopy (SEM), demonstrated that the HPP treatments can be potentially used to control both non-O157 and O157:H7 STECs in heat sensitive products. Published by Elsevier Ltd. C1 [Hsu, HsinYun; Wu, James Swi-Bea] Natl Taiwan Univ, Inst Food Sci & Technol, Taipei 106, Taiwan. [Hsu, HsinYun; Sheen, Shiowshuh; Sites, Joseph; Huang, Lihan] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov; jsbwu@ntu.edu.tw FU USDA/ARS/ERRC; National Science Council of Taiwan FX This project was supported by USDA/ARS/ERRC and National Science Council of Taiwan. We also thank Ms. Guoping Bao for her technical assistance in attaining the Scanning electron microscopy images. NR 29 TC 9 Z9 9 U1 5 U2 40 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD JUN PY 2014 VL 40 BP 25 EP 30 DI 10.1016/j.fm.2013.11.019 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA AC8YQ UT WOS:000332821400004 PM 24549194 ER PT J AU Brooks, EB Wynne, RH Thomas, VA Blinn, CE Coulston, JW AF Brooks, Evan B. Wynne, Randolph H. Thomas, Valerie A. Blinn, Christine E. Coulston, John W. TI On-the-Fly Massively Multitemporal Change Detection Using Statistical Quality Control Charts and Landsat Data SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Degradation; statistical process control; thinning; trajectory ID FOREST DISTURBANCE; TRENDS AB One challenge to implementing spectral change detection algorithms using multitemporal Landsat data is that key dates and periods are often missing from the record due to weather disturbances and lapses in continuous coverage. This paper presents a method that utilizes residuals from harmonic regression over years of Landsat data, in conjunction with statistical quality control charts, to signal subtle disturbances in vegetative cover. These charts are able to detect changes from both deforestation and subtler forest degradation and thinning. First, harmonic regression residuals are computed after fitting models to interannual training data. These residual time series are then subjected to Shewhart X-bar control charts and exponentially weighted moving average charts. The Shewhart X-bar charts are also utilized in the algorithm to generate a data-driven cloud filter, effectively removing clouds and cloud shadows on a location-specific basis. Disturbed pixels are indicated when the charts signal a deviation from data-driven control limits. The methods are applied to a collection of loblolly pine (Pinus taeda) stands in Alabama, USA. The results are compared with stands for which known thinning has occurred at known times. The method yielded an overall accuracy of 85%, with the particular result that it provided afforestation/deforestation maps on a per-image basis, producing new maps with each successive incorporated image. These maps matched very well with observed changes in aerial photography over the test period. Accordingly, the method is highly recommended for on-the-fly change detection, for changes in both land use and land management within a given land use. C1 [Brooks, Evan B.; Wynne, Randolph H.; Thomas, Valerie A.; Blinn, Christine E.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24060 USA. [Coulston, John W.] US Forest Serv, USDA, Southern Res Stn, Forest Inventory & Anal Unit, Knoxville, TN 37919 USA. RP Brooks, EB (reprint author), Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24060 USA. EM evbrooks@vt.edu FU U.S. Department of Agriculture Forest Service; Virginia Polytechnic Institute and State University (Virginia Tech) [10-CA-11330145-158]; U.S. Geological Survey [G12PC00073]; USDA National Institute of Food and Agriculture; USDA CSREES [VA-136614]; Department of Forest Resources and Environmental Conservation at Virginia Tech. FX Manuscript received January 16, 2013; revised May 21, 2013; accepted June 19, 2013. This work was supported in part by the U.S. Department of Agriculture Forest Service Cooperative Agreement with Virginia Polytechnic Institute and State University (Virginia Tech) under Grant 10-CA-11330145-158, by the Landsat Science Team under U.S. Geological Survey Contract G12PC00073, by the Pine Integrated Network: Education, Mitigation, and Adaptation Project (PINEMAP, Coordinated Agricultural Project funded in 2011 by the USDA National Institute of Food and Agriculture), by the McIntire-Stennis Cooperative Forestry Research Program through the USDA CSREES under Project VA-136614, and by the Department of Forest Resources and Environmental Conservation at Virginia Tech. NR 28 TC 15 Z9 15 U1 1 U2 37 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 EI 1558-0644 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD JUN PY 2014 VL 52 IS 6 BP 3316 EP 3332 DI 10.1109/TGRS.2013.2272545 PG 17 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA AC4QB UT WOS:000332504700024 ER PT J AU Brewer, LR Kubola, J Siriamornpun, S Herald, TJ Shi, YC AF Brewer, Lauren Renee Kubola, Jittawan Siriamornpun, Sirithon Herald, Thomas J. Shi, Yong-Cheng TI Wheat bran particle size influence on phytochemical extractability and antioxidant properties SO FOOD CHEMISTRY LA English DT Article DE Antioxidant; Particle size; Phenolic compounds; Wheat bran ID FRACTIONS; CAPACITY; EXTRACTS; ASSAYS; FLOUR; FLAVONOIDS; INCREASES; VARIETIES; QUALITY; PURPLE AB It is unknown if particle size plays a role in extracting health promoting compounds in wheat bran because the extraction of antioxidant and phenolic compounds with particle size reduction has not been well documented. In this study, unmilled whole bran (coarse treatment) was compared to whole bran milled to medium and fine treatments from the same wheat bran. Antioxidant properties (capacity, ability, power), carotenoids and phenolic compounds (phenolic acids, flavonoids, anthocyanins) were measured and compared. The ability of whole bran fractions of differing particle size distributions to inhibit free radicals was assessed using four in vitro models, namely, diphenylpicrylhydrazyl radical-scavenging activity, ferric reducing/antioxidant power (FRAP) assay, oxygen radical absorbance capacity (ORAC), and total antioxidant capacity. Significant differences in phytochemical concentrations and antioxidant properties were observed between whole bran fractions of reduced particle size distribution for some assays. The coarse treatment exhibited significantly higher antioxidant properties compared to the fine treatment; except for the ORAC value, in which coarse was significantly lower. For soluble and bound extractions, the coarse treatment was comparatively higher in total antioxidant capacity (426.72 mg ascorbic acid eq./g) and FRAP value (53.04 mu mol FeSO4/g) than bran milled to the finer treatment (314.55 ascorbic acid eq./g and 40.84 mu mol FeSO4/g, respectively). Likewise, the fine treatment was higher in phenolic acid (736 mg FAE/g), flavonoid (206.74 mu g catechin/g), anthocyanin (63.0 mu g/g), and carotenoid contents (beta carotene, 14.25 mu g/100 g; zeaxanthin, 35.21 mu g/100 g; lutein 174.59 mu g/ 100 g) as compared to the coarse treatment. An increase of surface area to mass increased the ORAC value by over 80%. With reduction in particle size, there was a significant increase in extracted anthocyanins, carotenoids and ORAC value. Particle size does effect the extraction of phytochemicals. (c) 2013 Elsevier Ltd. All rights reserved. C1 [Brewer, Lauren Renee; Shi, Yong-Cheng] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Kubola, Jittawan; Siriamornpun, Sirithon] Mahasarakham Univ, Dept Food Technol & Nutr, Maha Sarakham 44000, Thailand. [Herald, Thomas J.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Shi, YC (reprint author), Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. EM ycshi@ksu.edu FU USDA National Needs Graduate Fellowship Competitive from the National Institute of Food and Agriculture [2008-3842004773] FX This work was made possible through the assistance of Drs. Jeffrey Gwirtz, Dan Qiu, Priyadarshini Gadgil. Support for the student's Lauren Brewer training project was provided by the USDA National Needs Graduate Fellowship Competitive Grant No. 2008-3842004773 from the National Institute of Food and Agriculture. Support for the student's Jittawan Kubola research and international student exchange was provided by the Office of the Higher Education Commission, Thailand, under the program Strategic Scholarships for Frontier Research Network for the Joint Ph.D. Program Thai Doctoral degree for this research from Thailand. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable. The USDA is an equal opportunity provider and employer. This article is a US Government work and is in the public domain in the USA. This is contribution number 13-066-J from the Kansas Agricultural Experiment Station. NR 34 TC 9 Z9 11 U1 4 U2 66 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JUN 1 PY 2014 VL 152 BP 483 EP 490 DI 10.1016/j.foodchem.2013.11.128 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AB9RC UT WOS:000332132300067 PM 24444965 ER PT J AU Guo, MM Jin, TZ Wang, LX Scullen, OJ Sommers, CH AF Guo, Mingming Jin, Tony Z. Wang, Luxin Scullen, O. Joseph Sommers, Christopher H. TI Antimicrobial films and coatings for inactivation of Listeria innocua on ready-to-eat deli turkey meat SO FOOD CONTROL LA English DT Article DE RTE meat; Antimicrobial coating; Film; Lauric arginate; Nisin; Listeria innocua ID HIGH HYDROSTATIC-PRESSURE; ALLYL ISOTHIOCYANATE; POLY(LACTIC ACID); LAURIC ARGINATE; COMPOSITE FILMS; L-ARGININE; MONOCYTOGENES; NISIN; CHITOSAN; SURFACE AB Edible antimicrobial coating solutions incorporating chitosan, lauric arginate ester (LAE) and nisin were developed to reduce foodborne pathogen contamination on ready-to-eat (RTE) meats. RTE deli meat samples were directly coated with the solutions, or treated with solution-coated polylactic acid (PLA) films. The antimicrobial efficacy of the coatings and films against Listeria innocua inoculated onto the surface of RTE meat samples was investigated. Antimicrobial coatings with 1.94 mg/cm(2) of chitosan and 0.388 mg/cm(2) of LAE reduced L. innocua by ca. 4.5 log CFU/cm(2). Nisin (486 IU/cm(2)) showed less effectiveness than LAE (0.388 mg/cm(2)) and addition of nisin to the antimicrobial coatings or films containing LAE (0.388 mg/cm(2)) did not enhance the total antimicrobial effectiveness. Combining antimicrobial coatings or films with flash pasteurization (FP), which uses short burst of steam under pressure, further reduced L. innocua, achieving over a 5 log reduction. There was no significant difference in the effectiveness of antimicrobial films versus the coatings (p > 0.05). These data show the potential use of antimicrobial packaging alone, or in combination with FP, in preventing foodborne illness due to post-processing contamination of RTE meat products. Published by Elsevier Ltd. C1 [Guo, Mingming] Jiangnan Univ, Wuxi 214122, Jiangsu, Peoples R China. [Jin, Tony Z.; Scullen, O. Joseph; Sommers, Christopher H.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wang, Luxin] Auburn Univ, Auburn, AL 36849 USA. RP Jin, TZ (reprint author), 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 FU U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) Current Research Information System through ARS National Program 108 [1935-41000-092-00D] FX This study was funded by U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) Current Research Information System project No. 1935-41000-092-00D through ARS National Program 108. The authors wish to thank Anita Parameswaran and Guoping Bao for technical assistance. NR 39 TC 16 Z9 16 U1 4 U2 85 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUN PY 2014 VL 40 BP 64 EP 70 DI 10.1016/j.foodcont.2013.11.018 PG 7 WC Food Science & Technology SC Food Science & Technology GA AB3CW UT WOS:000331669900010 ER PT J AU Gehring, AG Paoli, GC Reed, SA Tu, SI Lindsay, JA AF Gehring, Andrew G. Paoli, George C. Reed, Sue A. Tu, Shu-I Lindsay, James A. TI Casamino Acids and Oxyrase enhance growth of Listeria monocytogenes in multi-pathogen enrichments SO FOOD CONTROL LA English DT Article DE Casamino Acids; Listeria monocytogenes; Mixed culture; Oxyrase; Pathogens; Rapid methods ID ESCHERICHIA-COLI O157-H7; SALMONELLA-ENTERICA; TYPHIMURIUM; O157/H7; ENZYME; FOODS; PRODUCTS; BACTERIA; SAMPLES; CELLS AB Rapid methods have been developed as relatively faster alternatives to plate culture for the detection of pathogenic bacteria in foods. However, since most rapid methods are subject to logistical limitations (e.g., sample volume size, analysis time, matrix effects) and/or a detection scheme with insufficient sensitivity needed to detect very low levels of bacteria in foods, culture enrichment is often employed to increase the concentration of targeted pathogens prior to detection. Multiplexed rapid detection platforms, capable of simultaneous detection of different bacteria in a single sample, necessitate co-enrichment (or mixed culture enrichment) of as many different targeted microorganisms as possible in a timely manner. This investigation compares the growth of four major foodborne pathogens (Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, and Yersinia enterocolitica) inoculated into pristine media or ground pork and enriched in various culture media. Initial results revealed that, after 24 h incubation, the growth of L monocytogenes (the slowest-growing pathogen examined) was increased by approximately 1-log by the supplementation of Universal Preenrichment Broth with Casamino Acids and/or Oxyrase. Overnight (24 h) growth of L monocytogenes in ground pork enrichment cultures was enhanced up to ca. 2-log by the addition of either Casamino Acids or Casamino Acids and Oxyrase for each of the tested growth media. Ultimately, an overnight culture of the inoculated pathogens in any of the selected media containing both Casamino Acids and Oxyrase was observed to yield target bacterial concentrations that were at sufficient levels (between 10e5 and 10e6 CFU/mL) for detection by most rapid methods. Published by Elsevier Ltd. C1 [Gehring, Andrew G.; Paoli, George C.; Reed, Sue A.; Tu, Shu-I] ARS, Microbial Biophys & Residue Chem Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Lindsay, James A.] ARS, USDA, George Washington Carver Ctr, Beltsville, MD 20705 USA. RP Gehring, AG (reprint author), ARS, Microbial Biophys & Residue Chem Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM andrew.gehring@ars.usda.gov NR 33 TC 1 Z9 1 U1 3 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUN PY 2014 VL 40 BP 93 EP 99 DI 10.1016/j.foodcont.2013.11.038 PG 7 WC Food Science & Technology SC Food Science & Technology GA AB3CW UT WOS:000331669900014 ER PT J AU Zhou, MM Kimbeng, CA Tew, TL Gravois, KA Pontif, M Bischoff, KP AF Zhou, M. M. Kimbeng, C. A. Tew, T. L. Gravois, K. A. Pontif, M. Bischoff, K. P. TI Logistic Regression Models to Aid Selection in Early Stages of Sugarcane Breeding SO SUGAR TECH LA English DT Article; Proceedings Paper CT 28th Congress of the International-Society-of-Sugar-Cane-Technologists CY JUN 24-27, 2013 CL Sao Paulo, BRAZIL SP Int Soc Sugar Cane Technologists DE Statistical decision support tool; Selection efficiency; Yield components AB In sugarcane breeding, visual appraisal is used to select for cane yield among seedlings (Stage I) and non-replicated clonal plots (Stage II). Genotype by environment interaction and interplot competition reduces selection efficiency. Although path coefficient analysis studies identified important yield components for indirect selection, currently there is little integration of that knowledge in models to improve early stage selection. This study demonstrates the use of logistic regression models as a statistical decision support tool that uses simultaneous selection for yield components (stalk number, stalk height, stalk diameter, estimable recoverable crystal (ERC) % cane) to help identify genotypes that produce high yield in non-replicated stages of sugarcane breeding. Data were collected from two Stage I populations grown at the USDA-ARS, Sugarcane Research Station at Houma and LSU AgCenter, Sugar Research Station, St. Gabriel, Louisiana, USA and two Stage II populations grown at Gingindlovu and Empangeni research stations, South African Sugarcane Research Institute, in South Africa. The data were analyzed using the logistic procedure of the statistical analysis system. Genotypes selected using the logistic regression models produced higher yield and larger values for yield components than those not selected. Stalk number was the most influential yield component while ERC% cane was the least. The logistic regression models identified the yield components that contributed significantly to yield for each population. The differences in variability within and among the populations provided guidance as to the precision of selection and therefore could indicate to the breeder the need for a higher or lower selection rate. Higher discriminating ability between selected and rejected genotypes indicated greater precision of selection. C1 [Zhou, M. M.] South African Sugarcane Res Inst, ZA-4300 Mt Edgecombe, South Africa. [Kimbeng, C. A.; Gravois, K. A.; Pontif, M.; Bischoff, K. P.] LSU AgCtr, Sugar Res Stn, St Gabriel, LA 70776 USA. [Tew, T. L.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. RP Zhou, MM (reprint author), South African Sugarcane Res Inst, P Bag X02, ZA-4300 Mt Edgecombe, South Africa. EM marvellous.zhou@sugar.org.za; ckimbeng@agcenter.lsu.edu; tom.tew@usda.gov; kgravois@agcenter.lsu.edu; mpontif@agcenter.lsu.edu; kbischoff@agcenter.lsu.edu NR 15 TC 2 Z9 2 U1 0 U2 12 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD JUN PY 2014 VL 16 IS 2 SI SI BP 150 EP 156 DI 10.1007/s12355-013-0266-1 PG 7 WC Agronomy SC Agriculture GA AA3CO UT WOS:000330970700005 ER PT J AU Hu, LH Pan, H Zhou, YH Hse, CY Liu, CG Zhang, BF Xu, B AF Hu, Lihong Pan, Hui Zhou, Yonghong Hse, Chung-Yun Liu, Chengguo Zhang, Baofang Xu, Bin TI Chemical Groups and Structural Characterization of Lignin via Thiol-Mediated Demethylation SO JOURNAL OF WOOD CHEMISTRY AND TECHNOLOGY LA English DT Article DE Biomaterials; demethylation; odorless thiol; nucleophilic substitution; lignin reactivity ID ARYL METHYL ETHERS; ODORLESS THIOLS; WHEAT-STRAW AB A new approach to increase the reactivity of lignin by thiol-mediated demethylation was investigated in this study. Demethylated lignin was characterized by the changes in its hydroxyl and methoxyl groups, molecular weight, and other properties using titration and spectroscopy methods including FT-IR, H-1 NMR, UV, and GPC. The total, phenolic, and aliphatic hydroxyl contents in lignin increased while the methoxyl content decreased after demethylation reaction, which indicated the occurrence of demethylation reaction. The results from FT-IR and UV analysis also indicated that the kraft lignin in this study contains mainly guaiacyl moiety and demethylated lignin has a higher guaiacyl content than original lignin. The average molecular weight of lignin decreased after demethylation reaction due to the cleavage of ether linkages in lignin macromolecules. No severe degradation of lignin was observed during the demethylation reaction. C1 [Hu, Lihong; Zhou, Yonghong; Liu, Chengguo; Xu, Bin] CAF, Res Inst New Technol, Inst Chem Ind Forestry Prod, Nanjing, Jiangsu, Peoples R China. [Hu, Lihong; Pan, Hui] Louisiana State Univ, Ctr Agr, Calhoun Res Stn, Calhoun, LA USA. [Hse, Chung-Yun] US Forest Serv, USDA, Southern Res Stn, Pineville, LA USA. [Zhang, Baofang] Univ Akron, Dept Polymer Sci, Akron, OH 44325 USA. RP Pan, H (reprint author), Nanjing Forestry Univ, Sch Chem Engn, 159 Longpan Rd, Nanjing 210037, Jiangsu, Peoples R China. EM Hpan@njfu.edu.cn FU International S&T Cooperation Program of China [2011DFA32440] FX The authors would like to thank the International S&T Cooperation Program of China (2011DFA32440) for their financial support during this investigation. NR 32 TC 9 Z9 9 U1 4 U2 104 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0277-3813 EI 1532-2319 J9 J WOOD CHEM TECHNOL JI J. Wood Chem. Technol. PD JUN 1 PY 2014 VL 34 IS 2 BP 122 EP 134 DI 10.1080/02773813.2013.844165 PG 13 WC Materials Science, Paper & Wood SC Materials Science GA 272MU UT WOS:000328465700004 ER PT J AU Dunn, TN Keenan, AH Thomas, AP Newman, JW Adams, SH AF Dunn, Tamara N. Keenan, Alison H. Thomas, Anthony P. Newman, John W. Adams, Sean H. TI A diet containing a nonfat dry milk matrix significantly alters systemic oxylipins and the endocannabinoid 2-arachidonoylglycerol (2-AG) in diet-induced obese mice SO NUTRITION & METABOLISM LA English DT Article ID EPOXIDE HYDROLASE DEFICIENCY; IMPROVES GLUCOSE-HOMEOSTASIS; ADIPOSE-TISSUE INFLAMMATION; INDUCED INSULIN-RESISTANCE; HIGH-CALCIUM DIET; WHEY-PROTEIN; ENERGY HOMEOSTASIS; DAIRY-PRODUCTS; CB1 RECEPTORS; BODY-WEIGHT AB Background: Diets rich in dairy and/or calcium (Ca) have been associated with reductions in adiposity and inflammation, but the mechanisms underlying this remain to be fully elucidated. Oxylipins and endocannabinoids are bioactive lipids, which influence energy homeostasis, adipose function, insulin signaling, and inflammation. Our objective was to determine if these metabolites associate with metabolic and inflammatory phenotypes stemming from dietary Ca and dairy in diet induced obese mice. Methods: In one study, C57BL6/J mice were fed high fat diets (45% energy) with varying dietary matrices for 12 weeks: soy protein and Ca adequate (0.5%; CONTROL), soy protein and high Ca (1.5%; HighCa), or nonfat-dry-milk based high Ca (NFDM). In a second study, mice were pre-fattened for 12 weeks on the CONTROL high fat diet, and then fed one of three high fat diets for an additional 8 weeks: CONTROL, HighCa, or NFDM. In both studies, adiposity and associated metabolic and inflammatory outcomes were measured and a targeted lipidomics analysis was performed on plasma collected during the post-absorptive condition. Results: As reported previously, mice fed NFDM had less body fat and reduced mRNA markers of adipose inflammation (p < 0.05) than CONTROL mice despite greater cumulative energy intake. Moreover, NFDM fed mice lipid mediator profiles were distinct from CONTROL and HighCa mice. NFDM fed mice showed elevated plasma monoacylglycerols (6 - 46% increase from CONTROL), including 2-arachidonoylglycerol (2-AG), and reduced fatty acid diols (8-75% decrease from CONTROL). Conclusions: Differences in specific plasma lipid mediator profiles reflect the metabolic and inflammatory phenotypes seen in NFDM feeding. C1 [Dunn, Tamara N.; Keenan, Alison H.; Thomas, Anthony P.; Newman, John W.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Newman, John W.; Adams, Sean H.] USDA ARS, Western Human Nutr Res Ctr, Obes & Metab Res Unit, Davis, CA USA. [Dunn, Tamara N.; Keenan, Alison H.; Thomas, Anthony P.; Newman, John W.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Newman, John W.; Adams, Sean H.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Newman, JW (reprint author), Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. EM john.newman@ars.usda.gov; sean.h.adams@ars.usda.gov FU USDA-ARS Projects [5306-51530-016-00D, 5306-51530-019-00]; National Dairy Council FX The authors would like to thank the UC Davis vivarium staff in helping care for the animals. Supported in part by intramural USDA-ARS Projects 5306-51530-016-00D and 5306-51530-019-00 and the National Dairy Council (grant administered by the Dairy Research Institute). USDA is an equal opportunity provider and employer. NR 73 TC 1 Z9 1 U1 2 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-7075 J9 NUTR METAB JI Nutr. Metab. PD MAY 30 PY 2014 VL 11 AR 24 DI 10.1186/1743-7075-11-24 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AK2HT UT WOS:000338240600001 PM 24963334 ER PT J AU Bifano, TD Ueti, MW Esteves, E Reif, KE Braz, GRC Scoles, GA Bastos, RG White, SN Daffre, S AF Bifano, Thais D. Ueti, Massaro W. Esteves, Eliane Reif, Kathryn E. Braz, Gloria R. C. Scoles, Glen A. Bastos, Reginaldo G. White, Stephen N. Daffre, Sirlei TI Knockdown of the Rhipicephalus microplus Cytochrome c Oxidase Subunit III Gene Is Associated with a Failure of Anaplasma marginale Transmission SO PLOS ONE LA English DT Article ID PROTECTIVE ANTIGEN SUBOLESIN; GLUTATHIONE-S-TRANSFERASE; IXODES-SCAPULARIS ACARI; CELL-LINE BME26; BOOPHILUS-MICROPLUS; DIFFERENTIAL EXPRESSION; SALIVARY-GLANDS; A-PHAGOCYTOPHILUM; OXIDATIVE STRESS; BABESIA-BOVIS AB Rhipicephalus microplus is an obligate hematophagous ectoparasite of cattle and an important biological vector of Anaplasma marginale in tropical and subtropical regions. The primary determinants for A. marginale transmission are infection of the tick gut, followed by infection of salivary glands. Transmission of A. marginale to cattle occurs via infected saliva delivered during tick feeding. Interference in colonization of either the tick gut or salivary glands can affect transmission of A. marginale to naive animals. In this study, we used the tick embryonic cell line BME26 to identify genes that are modulated in response to A. marginale infection. Suppression-subtractive hybridization libraries (SSH) were constructed, and five up-regulated genes {glutathione S-transferase (GST), cytochrome c oxidase sub III (COXIII), dynein (DYN), synaptobrevin (SYN) and phosphatidylinositol-3,4,5-triphosphate 3-phosphatase (PHOS)} were selected as targets for functional in vivo genomic analysis. RNA interference (RNAi) was used to determine the effect of tick gene knockdown on A. marginale acquisition and transmission. Although RNAi consistently knocked down all individually examined tick genes in infected tick guts and salivary glands, only the group of ticks injected with dsCOXIII failed to transmit A. marginale to naive calves. To our knowledge, this is the first report demonstrating that RNAi of a tick gene is associated with a failure of A. marginale transmission. C1 [Bifano, Thais D.; Esteves, Eliane; Daffre, Sirlei] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil. [Bifano, Thais D.; Reif, Kathryn E.; Bastos, Reginaldo G.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Ueti, Massaro W.; Scoles, Glen A.; White, Stephen N.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Braz, Gloria R. C.] Univ Fed Rio de Janeiro, Inst Quim, Dept Biochem, Rio De Janeiro, RJ, Brazil. [Braz, Gloria R. C.; Daffre, Sirlei] Inst Nacl Ciencia & Tecnol Entomol Mol, Rio De Janeiro, RJ, Brazil. RP Bifano, TD (reprint author), Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508 Sao Paulo, Brazil. EM thaisbifano@usp.br RI Esteves, Eliane/H-1037-2015; OI White, Stephen N./0000-0001-9255-6054 FU Sao Paulo Research Foundation (FAPESP) [2008/56408-0, 2010/50940-1, 2011/22124-8]; CNPq; CAPES; U.S. Department of Agriculture [ARS 5348-32000-033-00D] FX This work was supported by the Sao Paulo Research Foundation (FAPESP) grants 2008/56408-0, 2010/50940-1, 2011/22124-8, CNPq, CAPES and U.S. Department of Agriculture grant ARS 5348-32000-033-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 2 Z9 2 U1 3 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 30 PY 2014 VL 9 IS 5 AR e98614 DI 10.1371/journal.pone.0098614 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AK0KM UT WOS:000338101500074 PM 24878588 ER PT J AU El Bissati, K Zhou, Y Dasgupta, D Cobb, D Dubey, JP Burkhard, P Lanar, DE McLeod, R AF El Bissati, Kamal Zhou, Ying Dasgupta, Debleena Cobb, Drew Dubey, Jitender P. Burkhard, Peter Lanar, David E. McLeod, Rima TI Effectiveness of a novel immunogenic nanoparticle platform for Toxoplasma peptide vaccine in HLA transgenic mice SO VACCINE LA English DT Article DE Toxoplasma gondii; HLA-B7; Vaccine; Nanoparticles ID IFN-GAMMA; CONGENITAL TOXOPLASMOSIS; PROTECTIVE IMMUNITY; DENDRITIC CELLS; GONDII; EXPRESSION; RESISTANCE; RESPONSES; GRA7; INFECTION AB We created and produced a novel self-assembling nanoparticle platform for delivery of peptide epitopes that induces CD8(+) and CD4(+)T cells that are protective against Toxoplasma gondii infection. These selfassembling polypeptide nanoparticles (SAPNs) are composed of linear peptide (LP) monomers which contain two coiled-coil oligomerization domains, the dense granule 7 (GRA7(20-28) LPQFATAAT) peptide and a universal CD4(+)T cell epitope (derived from PADRE). Purified LPs assemble into nanoparticles with icosahedral symmetry, similar to the capsids of small viruses. These particles were evaluated for their efficacy in eliciting IFN-gamma by splenocytes of HLA-B(*)0702 transgenic mice and for their ability to protect against subsequent T. gondii challenge. This work demonstrates the feasibility of using this platform approach with a CD8(+) epitope that binds HLA-B7 and tests the biological activity of potentially protective peptides restricted by human major histocompatibility complex (HLA) class I molecules in HLA transgenic mice. (C) 2014 Elsevier Ltd. All rights reserved. C1 [El Bissati, Kamal; Zhou, Ying; McLeod, Rima] Univ Chicago, Dept Surg, Chicago, IL 60637 USA. [Dasgupta, Debleena; Lanar, David E.] Walter Reed Army Inst Res, Malaria Vaccine Branch, Silver Spring, MD 20910 USA. [Cobb, Drew] George Washington Univ, Washington, DC 20037 USA. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Burkhard, Peter] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA. [Burkhard, Peter] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA. RP El Bissati, K (reprint author), Univ Chicago, Dept Surg, 5841 S Maryland Ave, Chicago, IL 60637 USA. EM kelbissati@uchicago.edu FU DMID-NIAID [U01 A0177887, R01 27530] FX We gratefully thank Kevin Muite and Ani Solanki for technical help, Ernest Mui for helpful suggestions, Laura Knoll (Wisconsin) for the luciferase expressing parasites, and Boris Streipen (University of Georgia) for the RH-YFP parasites. We gratefully acknowledge support of this work from the Mann Cornwell, Engel and RooneyAlden, Lange!, Rosenthal and Jensen families. This work also was supported by DMID-NIAID U01 A0177887, R01 27530 (to RM), Knights Templar Eye Foundation (to KE), and The Research to Prevent Blindness Foundation (to RM). NR 33 TC 18 Z9 18 U1 1 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD MAY 30 PY 2014 VL 32 IS 26 BP 3243 EP 3248 DI 10.1016/j.vaccine.2014.03.092 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA AI4YL UT WOS:000336872500020 PM 24736000 ER PT J AU Geis-Asteggiante, L Nunez, A Lehotay, SJ Lightfield, AR AF Geis-Asteggiante, Lucia Nunez, Alberto Lehotay, Steven J. Lightfield, Alan R. TI Structural characterization of product ions by electrospray ionization and quadrupole time-of-flight mass spectrometry to support regulatory analysis of veterinary drug residues in foods SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; FRAGMENTATION PATHWAYS; ACCURATE MASS; ESI-MS; IDENTIFICATION; SULFONAMIDES; TRAP; CONFIRMATION; ANTIBIOTICS; DISSOCIATION AB RATIONALE Monitoring of veterinary drug residues in foods is often conducted using liquid chromatography/tandem mass spectrometry (LC/MS/MS). Results have high economic stakes for producers, but the ions monitored are usually selected due to signal intensities without structural interpretation. In this study, the ion transitions were characterized by high-resolution mass spectrometry. METHODS The 62 veterinary drugs from the LC/MS/MS method consisted of sulfonamides, beta-lactams, phenicols, macrolides, tetracyclines, fluoroquinolones, non-steroidal anti-inflammatory drugs (NSAIDs), and corticosteroids. They were individually infused into a quadrupole time-of-flight (Q-TOF) mass spectrometer using electrospray ionization (ESI) operated in positive mode. The MS and collision-induced dissociation (CID) MS/MS spectra for each analyte were obtained for structural elucidation. The Q-TOF instrument was calibrated to obtain a mass accuracy error RESULTS The use of high-resolution ESI-Q-TOF-MS for the generation of the MS/MS product ions allowed for the determination of chemical formulae for the analytes, some of which led to new findings. Assigned structures were based on rational interpretation of the most stable possible products with comparison with the scientific literature. In difficult cases, isotopically labeled drugs or hydrogen/deuterium (H/D) exchange experiments were used to help confirm the structures of the product ions. CONCLUSIONS The use of ESI-Q-TOF-MS in this study has allowed structure elucidation of 186 MS/MS product ions previously selected for the LC/MS/MS analysis of 62 veterinary drugs. This serves to reduce the chances of false positives and negatives in the monitoring program, and provides justification and defense in regulatory enforcement actions. Published in 2014. This article is a US Government work and is in the public domain in the USA. C1 [Geis-Asteggiante, Lucia; Nunez, Alberto; Lehotay, Steven J.; Lightfield, Alan R.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Geis-Asteggiante, Lucia] UdelaR, Fac Quim, DQO, Catedra Farmacognosia & Prod Nat, Montevideo 12800, Uruguay. RP Nunez, A (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM alberto.nunez@ars.usda.gov FU US-Israel Binational Agricultural Research and Development Fund Grant [US-4273-09] FX This work was conducted under Interagency Agreement No. #60-1935-9-031 between the Agricultural Research Service and the Food Safety and Inspection Service. This work was also supported by the US-Israel Binational Agricultural Research and Development Fund Grant US-4273-09. NR 52 TC 10 Z9 10 U1 3 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0951-4198 EI 1097-0231 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PD MAY 30 PY 2014 VL 28 IS 10 BP 1061 EP 1081 DI 10.1002/rcm.6871 PG 21 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA AE2OK UT WOS:000333812400001 PM 24711270 ER PT J AU Dubey, JP Black, SS Verma, SK Calero-Bernal, R Morris, E Hanson, MA Cooley, AJ AF Dubey, J. P. Black, S. S. Verma, S. K. Calero-Bernal, R. Morris, E. Hanson, M. A. Cooley, A. J. TI Sarcocystis neurona schizonts-associated encephalitis, chorioretinitis, and myositis in a two-month-old dog simulating toxoplasmosis, and presence of mature sarcocysts in muscles SO VETERINARY PARASITOLOGY LA English DT Article DE Sarcocystis neurona; Dog; Schizonts; Sarcocysts; Immunoreactivity; Electron microscopy ID OTTER ENHYDRA-LUTRIS; EQUINE PROTOZOAL MYELOENCEPHALITIS; OPOSSUMS DIDELPHIS-VIRGINIANA; RACCOONS PROCYON-LOTOR; INTERMEDIATE HOST; NEOSPORA-CANINUM; LIFE-CYCLE; INFECTION; TRANSMISSION; DISEASE AB Sarcocystis neurona is an unusual species of the genus Sarcocystis. Opossums (Didelphis virginianus, D. albiventris) are the definitive hosts and several other species, including dogs, cats, marine mammals, and horses are intermediate or aberrant hosts. Sarcocysts are not known to form in aberrant hosts. Sarcocystis neurone causes fatal disease in horses (Equine Protozoal Myeloencephalitis, EPM). There are numerous reports of fatal EPM-like infections in other species, usually with central nervous system signs and associated with the schizont stage of S. neurona. Here, we report fatal disseminated S. neurona infection in a nine-weekold golden retriever dog from Mississippi, USA. Protozoal merozoites were identified in smears of the cerebrospinal fluid. Microscopically, lesions and protozoa were identified in eyes, tongue, heart, liver, intestines, nasal turbinates, skeletal muscle and brain, which reacted intensely with S. neurone polyclonal antibodies. Mature sarcocysts were seen in sections of muscles. These sarcocysts were ultrastructurally similar to those of S. neurona from experimentally infected animals. These data suggest that the dog is another intermediate host for S. neurone. Data suggest that the dog was transplacentally infected. (C) 2014 Elsevier B.V. All rights reserved. C1 [Dubey, J. P.; Verma, S. K.; Calero-Bernal, R.] Agr Res Serv, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Black, S. S.; Cooley, A. J.] Mississippi State Univ, Coll Vet Med, Mississippi State, MS 39762 USA. [Morris, E.; Hanson, M. A.] Joint Pathol Ctr, Vet Pathol Serv, Silver Spring, MD 20910 USA. RP Dubey, JP (reprint author), Agr Res Serv, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 32 TC 7 Z9 7 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD MAY 28 PY 2014 VL 202 IS 3-4 BP 194 EP 200 DI 10.1016/j.vetpar.2014.02.055 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AI4XT UT WOS:000336870600014 PM 24680604 ER PT J AU Lucas, AS Swecker, WS Lindsay, DS Scaglia, G Neel, JPS Elvinger, FC Zajac, AM AF Lucas, Aaron S. Swecker, William S., Jr. Lindsay, David S. Scaglia, Guillermo Neel, James P. S. Elvinger, Francois C. Zajac, Anne M. TI A study of the level and dynamics of Eimeria populations in naturally infected, grazing beef cattle at various stages of production in the Mid-Atlantic USA SO VETERINARY PARASITOLOGY LA English DT Article DE Eimeria; Cattle; Epidemiology ID GEORGIA USA; CALVES; COCCIDIA; WISCONSIN; PASTURE; OOCYSTS; COWS AB There is little information available on the species dynamics of eimerian parasites in grazing cattle in the central Appalachian region of the United States. Therefore, the objective of this study was to describe the level of infection and species dynamics of Eimeria spp. in grazing beef cattle of various age groups over the course of a year in the central Appalachian region. Rectal fecal samples were collected from male and female calves (n = 72) monthly from May through October 2005, heifers only (n=36) monthly from November 2005 to April 2006, and cows (n = 72) in May, July, and September, 2005. Eimeria spp. oocysts were seen in 399 of 414(96%) fecal samples collected from the calves from May through October. Fecal oocysts counts (FOC) in the calves were lower (P < 0.05) in May than all other months and no significant differences were detected from June through September. Eimeria spp. oocysts were detected in 198 of 213 (92%) of fecal samples collected from the 36 replacement heifers monthly from November to April and monthly mean FOC did not differ during this time period. The prevalence of oocyst shedding increased to 100% in calves in September and remained near 100% in the replacement heifers during the sampling period. Eimeria spp. oocysts were also detected in 150 of 200 (75%) samples collected in May, July, and September from the cows and mean FOC did not differ significantly over the sampling period. Eimeria spp. composition was dominated by Eimeria bovis in fecal samples collected from calves, replacement heifers and cows. Mixed Eimeria spp. infections were, however, common in all groups and 13 Eimeria spp. oocysts were identified throughout the sampling period. (C) 2014 Elsevier B.V. All rights reserved. C1 [Lucas, Aaron S.] Luray Clin Vet Med, Luray, VA 22835 USA. [Swecker, William S., Jr.; Elvinger, Francois C.] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Large Anim Clin Sci, Blacksburg, VA 24061 USA. [Lindsay, David S.; Zajac, Anne M.] Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. [Scaglia, Guillermo] Louisiana State Univ AgCtr, Iberia Res Stn, Jeanerette, LA 70544 USA. [Neel, James P. S.] USDA ARS GRL, El Reno, OK 73036 USA. RP Zajac, AM (reprint author), Virginia Tech, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. EM azajac@vt.edu RI Lindsay, David/G-8891-2016; Swecker, William/K-8186-2016 OI Lindsay, David/0000-0002-0592-8321; Swecker, William/0000-0003-4044-7563 FU USDA-ARS FX The research was part of a regional initiative, Pasture-Based Beef Systems for Appalachia, funded in part by USDA-ARS. The authors would like to thank the staff of the Shenandoah Valley Agricultural Research and Extension Center for coordinating animal husbandry and Laboratory for Study Design and Statistical Service for their support in statistical consulting. NR 25 TC 2 Z9 2 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD MAY 28 PY 2014 VL 202 IS 3-4 BP 201 EP 206 DI 10.1016/j.vetpar.2014.02.053 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AI4XT UT WOS:000336870600015 PM 24680603 ER PT J AU Dubey, JP Dennis, PM Verma, SK Choudhary, S Ferreira, LR Oliveira, S Kwok, OCH Butler, E Carstensen, M Su, C AF Dubey, J. P. Dennis, P. M. Verma, S. K. Choudhary, S. Ferreira, L. R. Oliveira, S. Kwok, O. C. H. Butler, E. Carstensen, M. Su, C. TI Epidemiology of toxoplasmosis in white tailed deer (Odocoileus virginianus): Occurrence, congenital transmission, correlates of infection, isolation, and genetic characterization of Toxoplasma gondii SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; White tailed deer; Serology; Epidemiology; Isolation ID BOBCAT LYNX-RUFUS; NEOSPORA-CANINUM; HIGH PREVALENCE; ANTIBODIES; SEROPREVALENCE; PENNSYLVANIA; MINNESOTA; LINEAGES; WILDLIFE; DISEASE AB The prevalence of Toxoplasma gondii in white tailed deer (WTD) in the USA is high but little is known of the epidemiology of toxoplasmosis in this host. In the present study, we compared T. gondii seroprevalence from 749 WTD collected in 2012 and 2013 from a Metropolitan Park in Ohio and 487 WTD deer shot in Minnesota during 2008, 2009, and 2010. Serum samples were tested for antibodies to T. gondii by the modified agglutination test (cut-off titer, 25). Additionally myocardial samples from 123 seropositive WTD from Ohio were digested in pepsin and the digests were bioassayed for the isolation of T. gondii. Furthermore, to estimate transplacental rate of transmission, brains from 155 fetuses (included twins) from 148 deer from Minnesota were bioassayed in mice for the isolation of viable T. gondii. Seroprevalence of T. gondii varied with the year of collection, geography, and the age of deer. Of the Ohio deer sampled in 2012 and 2013 seroprevalences for the two years were similar (73.4% and 75.7%, respectively); remarkably 150 (66.1%) of 227 deer of <1 year of age were seropositive. Of the Minnesota deer, seroprevalence was lowest for the year 2008 (14.8%, 26/175) versus 2009 (27.7%, 59/213), and 2010 (25.2%, 25/99), thought to be related to environmental temperatures. Viable T. gondii was isolated in mice from the myocardium of four WTD from Ohio, and brain of one WTD fetus from Minnesota. Tachyzoites from infected mouse tissues were further propagated in cell culture. The DNA isolated from culture-derived tachyzoites of these five T. gondii isolates was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Four genotypes were found, including ToxoDB genotype no. 1 (Type II), no. 2 (Type III), no. 3 (Type II variant) and no. 146. Results indicate fluctuating seroprevalence, probably related to weather and warrant further epidemiological studies. (C) 2014 Elsevier B.V. All rights reserved. C1 [Dubey, J. P.; Verma, S. K.; Choudhary, S.; Ferreira, L. R.; Oliveira, S.; Kwok, O. C. H.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Dennis, P. M.] Cleveland Metropk Zoo, Cleveland, OH 44109 USA. [Butler, E.; Carstensen, M.] Minnesota Dept Nat Resources, Div Wildlife, Forest Lake, MN 55025 USA. [Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov RI Dennis, Patricia/A-2081-2015 NR 29 TC 7 Z9 8 U1 4 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD MAY 28 PY 2014 VL 202 IS 3-4 BP 270 EP 275 DI 10.1016/j.vetpar.2014.01.006 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AI4XT UT WOS:000336870600024 PM 24582734 ER PT J AU Muramoto, J Gliessman, SR Koike, ST Shennan, C Bull, CT Klonsky, K Swezey, S AF Muramoto, Joji Gliessman, Stephen R. Koike, Steven T. Shennan, Carol Bull, Carolee T. Klonsky, Karen Swezey, Sean TI Integrated Biological and Cultural Practices Can Reduce Crop Rotation Period of Organic Strawberries SO AGROECOLOGY AND SUSTAINABLE FOOD SYSTEMS LA English DT Article DE strawberries (Fragaria x ananassa Duch.); crop rotation; Verticillium dahliae; broccoli (Brassica oleracea L. (italica group)); organic farming ID FRAGARIA X ANANASSA; CALIFORNIA STRAWBERRIES; VERTICILLIUM-DAHLIAE; SOIL SICKNESS; FIELD-RESISTANCE; REPLANT DISEASE; AUTOTOXICITY; MANAGEMENT; WILT; SEEDLINGS AB A team of researchers conducted a replicated on-farm experiment with the break period between strawberry crops (continuous strawberries with broccoli residue incorporation, one-year break, two-year break, three-year break, and seven-year break) as the main plot and cultivar as the split plot in Moss Landing, Central Coastal California. We hypothesized that the use of non-host rotation crops for Verticillium wilt plus bio-fumigation with broccoli, incorporation of mustard cover crop residues, use of relatively resistant strawberry cultivars, and compost application would suppress disease sufficiently to grow strawberries successfully in rotation every two or three years. Although a positive correlation between break period and marketable fruit yield existed, integrated use of biological and cultural practices allowed one to three-year breaks to have a statistically similar yield as seven-year break for this low Verticillium dahliae pressure field over a five-year period. C1 [Muramoto, Joji; Gliessman, Stephen R.] Univ Calif Santa Cruz, PICA, Santa Cruz, CA 95064 USA. [Muramoto, Joji; Gliessman, Stephen R.; Shennan, Carol] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA USA. [Shennan, Carol; Swezey, Sean] Univ Calif Santa Cruz, CASFS, Santa Cruz, CA 95064 USA. [Bull, Carolee T.] ARS, USDA, Salinas, CA USA. [Klonsky, Karen] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA 95616 USA. RP Muramoto, J (reprint author), Univ Calif Santa Cruz, Dept Environm Studies, 1156 High St, Santa Cruz, CA 95064 USA. EM joji@ucsc.edu RI Shennan, Carol/I-1694-2013 OI Shennan, Carol/0000-0001-6401-5007 FU North American Strawberry Growers Association; California Strawberry Commission; Ruth and Alfred Heller Chair in Agroecology at UCSC; Center for Agroecology and Sustainable Food Systems at UCSC; Halliday Foundation; Organic Farming Research Foundation; USDA Integrated Organic Program [2004-51300-02232]; USDA [2004-34424-14408]; Central Coast Regional Water Quality Control Board Non-Point Source Pollution Fund of the Community Foundation for Monterey County FX The project was partially funded by the North American Strawberry Growers Association, the California Strawberry Commission, the Ruth and Alfred Heller Chair in Agroecology at UCSC, the Center for Agroecology and Sustainable Food Systems at UCSC, the Halliday Foundation, the Organic Farming Research Foundation, USDA Integrated Organic Program 2004-51300-02232, USDA Special Grant 2004-34424-14408, and the Central Coast Regional Water Quality Control Board Non-Point Source Pollution Fund of the Community Foundation for Monterey County. Weather data were kindly provided by the Elkhorn Slough National Estuarine Research Reserve. NR 65 TC 5 Z9 5 U1 7 U2 47 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 520 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 2168-3565 EI 2168-3573 J9 AGROECOL SUST FOOD JI Agroecol. Sustain. Food Syst. PD MAY 28 PY 2014 VL 38 IS 5 BP 603 EP 631 DI 10.1080/21683565.2013.878429 PG 29 WC Agriculture, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY SC Agriculture; Science & Technology - Other Topics GA AE3IJ UT WOS:000333869900006 ER PT J AU Kianian, PMA Kianian, SF AF Kianian, Penny M. A. Kianian, Shahryar F. TI Mitochondrial dynamics and the cell cycle SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE nuclear-mitochondrial communication; mitochondrial dynamics; cell cycle; mitosis; meiosis; alloplasmic ID CYTOPLASMIC MALE-STERILITY; NICOTIANA-SYLVESTRIS; COMPLEX-I; ZEA-MAYS; 3-DIMENSIONAL RECONSTRUCTION; GAMETOPHYTE DEVELOPMENT; SPERM CELLS; ARABIDOPSIS; NUCLEAR; DIVISION AB Nuclear-mitochondrial (NM) communication impacts many aspects of plant development including vigor, sterility, and viability. Dynamic changes in mitochondrial number, shape, size, and cellular location takes place during the cell cycle possibly impacting the process itself and leading to distribution of this organelle into daughter cells. The genes that underlie these changes are beginning to be identified in model plants such as Arabidopsis. In animals disruption of the drp1 gene, a homolog to the plant drp3A and drp3B, delays mitochondrial division. This mutation results in increased aneuploidy due to chromosome mis-segregation. It remains to be discovered if a similar outcome is observed in plants. Alloplasmic lines provide an opportunity to understand the communication between the cytoplasmic organelles and the nucleus. Examples of studies in these lines, especially from the extensive collection in wheat, point to the role of mitochondria in chromosome movement, pollen fertility and other aspects of development. C1 [Kianian, Penny M. A.] Univ Minnesota, Dept Hort Sci, St Paul, MN USA. [Kianian, Shahryar F.] ARS, Cereal Dis Lab, USDA, St Paul, MN 55108 USA. RP Kianian, SF (reprint author), ARS, Cereal Dis Lab, USDA, 1551 Lindig Ave, St Paul, MN 55108 USA. EM shahryar.kianian@ars.usda.gov FU National Science Foundation-PGRP [IOS-1025881] FX This work was supported by a grant from the National Science Foundation-PGRP IOS-1025881. NR 58 TC 1 Z9 1 U1 4 U2 29 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 27 PY 2014 VL 5 AR 222 DI 10.3389/fpls.2014.00222 PG 6 WC Plant Sciences SC Plant Sciences GA AL3IY UT WOS:000339021800001 PM 24904617 ER PT J AU Gillman, JD Stacey, MG Cui, YY Berg, HR Stacey, G AF Gillman, Jason D. Stacey, Minviluz G. Cui, Yaya Berg, Howard R. Stacey, Gary TI Deletions of the SACPD-C locus elevate seed stearic acid levels but also result in fatty acid and morphological alterations in nitrogen fixing nodules SO BMC PLANT BIOLOGY LA English DT Article DE Soybean (Glycine max); Stearic acid; Fatty acid composition; Radiation mutagenesis; Comparative genome hybridization; Nodulation ID CARRIER-PROTEIN-DESATURASE; LOTUS-JAPONICUS; GENE-EXPRESSION; SYMBIOSOME MEMBRANE; SOYBEAN SEED; GLYCINE-MAX; ARABIDOPSIS; MUTATIONS; OIL; SENESCENCE AB Background: Soybean (Glycine max) seeds are the primary source of edible oil in the United States. Despite its widespread utility, soybean oil is oxidatively unstable. Until recently, the majority of soybean oil underwent chemical hydrogenation, a process which also generates trans fats. An alternative to chemical hydrogenation is genetic modification of seed oil through identification and introgression of mutant alleles. One target for improvement is the elevation of a saturated fat with no negative cardiovascular impacts, stearic acid, which typically constitutes a minute portion of seed oil (similar to 3%). Results: We examined radiation induced soybean mutants with moderately increased stearic acid (10-15% of seed oil, similar to 3-5 X the levels in wild-type soybean seeds) via comparative whole genome hybridization and genetic analysis. The deletion of one SACPD isoform encoding gene (SACPD-C) was perfectly correlated with moderate elevation of seed stearic acid content. However, SACPD-C deletion lines were also found to have altered nodule fatty acid composition and grossly altered morphology. Despite these defects, overall nodule accumulation and nitrogen fixation were unaffected, at least under laboratory conditions. Conclusions: Although no yield penalty has been reported for moderate elevated seed stearic acid content in soybean seeds, our results demonstrate that genetic alteration of seed traits can have unforeseen pleiotropic consequences. We have identified a role for fatty acid biosynthesis, and SACPD activity in particular, in the establishment and maintenance of symbiotic nitrogen fixation. C1 [Gillman, Jason D.] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [Stacey, Minviluz G.; Cui, Yaya; Stacey, Gary] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Stacey, Minviluz G.; Cui, Yaya; Stacey, Gary] Univ Missouri, Div Biochem, Columbia, MO 65211 USA. [Berg, Howard R.] Donald Danforth Plant Sci Ctr, St Louis, MO USA. RP Gillman, JD (reprint author), Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM Jason.Gillman@ars.usda.gov RI Berg, R. Howard/M-2839-2013 OI Berg, R. Howard/0000-0001-5081-2769 FU USDA-Agricultural Research Service; Plant Genome Program, National Science Foundation [IOS1127083] FX The authors would like to express our gratitude to Dr. Kristin Bilyeu for the generous donation of mutagenized populations for forward genetic screening. We would also like to thank Dr. Walter Fehr for the kind donation of A6 and 'FA8077' as well as helpful discussions. We are also appreciative of Dr. Toyoaki Anai and Legumebase for providing lines MM106, M25 and KK24. FAM94-4 and Dare seeds were kindly provided by Dr. Robert G. Upchurch. The authors also acknowledge the kind gift of the B. japonicum inoculum by Novozymes, Inc. We are very grateful to the following MU faculty and staff members who provided invaluable support in obtaining the data presented in this paper: Dr. Jay J. Thelen and Dr. Judy D. Wall for giving us access to their GC equipment; Dr. David W. Emerich for his technical support and suggestions in performing the nitrogen fixation assays; Kerry Clark for invaluable field assistance; Dr. Mingjie Chen for advise and discussions on FA extraction and analysis; and Grant M. Zane for assistance in running the GC machine. The authors would like to acknowledge the superb technical assistance of USDA-ARS technician Jeremy Mullis. This research was supported by the USDA-Agricultural Research Service and by grant #IOS1127083 from the Plant Genome Program, National Science Foundation (to GS). NR 64 TC 6 Z9 6 U1 1 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 27 PY 2014 VL 14 AR 143 DI 10.1186/1471-2229-14-143 PG 18 WC Plant Sciences SC Plant Sciences GA AK1DR UT WOS:000338156000001 PM 24886084 ER PT J AU Webb, NP Okin, GS Brown, S AF Webb, Nicholas P. Okin, Gregory S. Brown, Shannon TI The effect of roughness elements on wind erosion: The importance of surface shear stress distribution SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article ID ATMOSPHERIC DUST CYCLE; CHIHUAHUAN DESERT; DOMINATED LANDSCAPES; AEOLIAN TRANSPORT; DRAG PARTITION; NEW-MEXICO; VEGETATION; MODELS; FIELD; REPRESENTATION AB Representation of surface roughness effects on aeolian sediment transport is a key source of uncertainty in wind erosion models. Drag partitioning schemes are used to account for roughness by scaling the soil entrainment threshold by the ratio of shear stress on roughness elements to that on the vegetated land surface. This approach does not explicitly account for the effects of roughness configuration, which may be important for sediment flux. Here we investigate the significance of roughness configuration for aeolian sediment transport, the ability of drag partitioning approaches to represent roughness configuration effects, and the implications for model accuracy. We use wind tunnel measurements of surface shear stress distributions to calculate sediment flux for a suite of roughness configurations, roughness densities, and wind velocities. Roughness configuration has a significant effect on sediment flux, influencing estimates by more than 1 order of magnitude. Measured and modeled drag partitioning approaches overestimate the predicted flux by 2 to 3 orders of magnitude. The drag partition is sensitive to roughness configuration, but current models cannot effectively represent this sensitivity. The effectiveness of drag partitioning approaches is also affected by estimates of the aerodynamic roughness height used to calculate wind shear velocity. Unless the roughness height is consistent with the drag partition, resulting fluxes can show physically implausible patterns. These results should make us question current assessments of the magnitude of vegetated dryland dust emissions. Representing roughness effects on surface shear stress distributions will reduce uncertainty in quantifying wind erosion, enabling better assessment of its impacts and management solutions. C1 [Webb, Nicholas P.] ARS Jornada Expt Range, USDA, NMSU, Las Cruces, NM 88003 USA. [Okin, Gregory S.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Brown, Shannon] Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada. RP Webb, NP (reprint author), ARS Jornada Expt Range, USDA, NMSU, MSC 3 JER, Las Cruces, NM 88003 USA. EM nwebb@nmsu.edu FU Jornada Basin LTER [NSF DEB 12358218]; NSF [EAR-1148334] FX We thank W.G. Nickling and J.A. Gillies for their contributions to the wind tunnel experiments and manuscript review. We also thank the two anonymous reviewers for their constructive comments on the manuscript. This work was supported by the Jornada Basin LTER (NSF DEB 12358218) and NSF grant EAR-1148334. NR 47 TC 11 Z9 12 U1 3 U2 28 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD MAY 27 PY 2014 VL 119 IS 10 BP 6066 EP 6084 DI 10.1002/2014JD021491 PG 19 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AJ5YQ UT WOS:000337766600021 ER PT J AU Nayduch, D Lee, MB Saski, CA AF Nayduch, Dana Lee, Matthew B. Saski, Christopher A. TI The Reference Transcriptome of the Adult Female Biting Midge (Culicoides sonorensis) and Differential Gene Expression Profiling during Teneral, Blood, and Sucrose Feeding Conditions SO PLOS ONE LA English DT Article ID EPIZOOTIC HEMORRHAGIC-DISEASE; MOSQUITO AEDES-AEGYPTI; RNA-SEQ; DIPTERA-CERATOPOGONIDAE; ANOPHELES-GAMBIAE; VITELLOGENESIS; BLUETONGUE; PROTEIN; VECTOR; AGRICULTURE AB Unlike other important vectors such as mosquitoes and sandflies, genetic and genomic tools for Culicoides biting midges are lacking, despite the fact that they vector a large number of arboviruses and other pathogens impacting humans and domestic animals world-wide. In North America, female Culicoides sonorensis midges are important vectors of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV), orbiviruses that cause significant disease in livestock and wildlife. Libraries of tissue-specific transcripts expressed in response to feeding and oral orbivirus challenge in C. sonorensis have previously been reported, but extensive genome-wide expression profiling in the midge has not. Here, we successfully used deep sequencing technologies to construct the first adult female C. sonorensis reference transcriptome, and utilized genome-wide expression profiling to elucidate the genetic response to blood and sucrose feeding over time. The adult female midge unigene consists of 19,041 genes, of which less than 7% are differentially expressed during the course of a sucrose meal, while up to 52% of the genes respond significantly in blood-fed midges, indicating hematophagy induces complex physiological processes. Many genes that were differentially expressed during blood feeding were associated with digestion (e.g. proteases, lipases), hematophagy (e.g., salivary proteins), and vitellogenesis, revealing many major metabolic and biological factors underlying these critical processes. Additionally, key genes in the vitellogenesis pathway were identified, which provides the first glimpse into the molecular basis of anautogeny for C. sonorensis. This is the first extensive transcriptome for this genus, which will serve as a framework for future expression studies, RNAi, and provide a rich dataset contributing to the ultimate goal of informing a reference genome assembly and annotation. Moreover, this study will serve as a foundation for subsequent studies of genome-wide expression analyses during early orbivirus infection and dissecting the molecular mechanisms behind vector competence in midges. C1 [Nayduch, Dana; Lee, Matthew B.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. [Saski, Christopher A.] Clemson Univ, Genom Inst, Clemson, SC 29634 USA. RP Saski, CA (reprint author), Clemson Univ, Genom Inst, Clemson, SC 29634 USA. EM saski@clemson.edu FU USDA-ARS National Program 104 [5430-32000-003]; Clemson University [58-5430-2-313] FX This work was funded by USDA-ARS National Program 104, Project number 5430-32000-003, and a Specific Cooperative Agreement with Clemson University, Agreement 58-5430-2-313. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 5 Z9 5 U1 2 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 27 PY 2014 VL 9 IS 5 AR e98123 DI 10.1371/journal.pone.0098123 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI5NG UT WOS:000336914100034 PM 24866149 ER PT J AU Xu, XZ Wang, HR Jiang, L Wang, XN Payne, SA Zhu, JY Li, RP AF Xu, Xuezhu Wang, Haoran Jiang, Long Wang, Xinnan Payne, Scott A. Zhu, J. Y. Li, Ruipeng TI Comparison between Cellulose Nanocrystal and Cellulose Nanofibril Reinforced Poly(ethylene oxide) Nanofibers and Their Novel Shish-Kebab-Like Crystalline Structures SO MACROMOLECULES LA English DT Article ID ORDERED POLYMER NANOFIBERS; BENDING INSTABILITY; COMPOSITES; POLYPROPYLENE; WHISKERS; FIBERS; MICROSTRUCTURES; NANOCOMPOSITES; POLYETHYLENE; MELT AB Poly(ethylene oxide) (PEO) nanofiber mats were produced by electrospinning. Biobased cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs) as reinforcement nanofillers were also added to the polymer to produce composite nanofiber mats. The effects of the two cellulose nanofillers on the rheological properties of the PEO solutions and the microstructure, crystallization, and mechanical properties of the mats were systematically compared. The microstructural disparity between the CNCs and CNFs led to significant differences in the solution viscosity, nanofiber morphology and microstructure of the composite nanofiber mats. A unique shish-kebab-like crystalline structure was discovered in both pure and filled PEO nanofibers. Both CNCs and CNFs showed strong reinforcing effects on the nanofiber mats. C1 [Xu, Xuezhu; Wang, Haoran; Jiang, Long; Wang, Xinnan] N Dakota State Univ, Dept Mech Engn, Fargo, ND 58102 USA. [Xu, Xuezhu; Wang, Xinnan] N Dakota State Univ, Program Mat & Nanotechnol, Fargo, ND 58102 USA. [Payne, Scott A.] N Dakota State Univ, Electron Microscopy Core Facil, Fargo, ND 58102 USA. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Li, Ruipeng] Cornell Univ, CHESS Wilson Lab, Ithaca, NY 14853 USA. RP Jiang, L (reprint author), N Dakota State Univ, Dept Mech Engn, Fargo, ND 58102 USA. EM long.jiang@ndsu.edu RI Li, Ruipeng/A-3691-2014; Wang, Haoran/G-1942-2011; Jiang, Long/D-6172-2017 OI Li, Ruipeng/0000-0001-8176-3138; Wang, Haoran/0000-0002-0769-600X; Jiang, Long/0000-0002-1781-134X FU North Dakota EPSCoR; US Forest Service FX Financial supports from North Dakota EPSCoR and US Forest Service are greatly appreciated. Ms. Chenguang Long from Industrial and Manufacturing Engineering Department of NDSU is appreciated for conducting some of the experiments. NR 45 TC 27 Z9 27 U1 14 U2 128 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0024-9297 EI 1520-5835 J9 MACROMOLECULES JI Macromolecules PD MAY 27 PY 2014 VL 47 IS 10 BP 3409 EP 3416 DI 10.1021/ma402627j PG 8 WC Polymer Science SC Polymer Science GA AI1VM UT WOS:000336643300020 ER PT J AU Han, LJ Sapozhnikova, Y Lehotay, SJ AF Han, Lijun Sapozhnikova, Yelena Lehotay, Steven J. TI Streamlined sample cleanup using combined dispersive solid-phase extraction and in-vial filtration for analysis of pesticides and environmental pollutants in shrimp SO ANALYTICA CHIMICA ACTA LA English DT Article DE Filter-vial dispersive solid-phase extraction; Pesticide residue analysis; Environmental contaminants; QuEChERS; Shrimp ID TANDEM MASS-SPECTROMETRY; POLYCYCLIC AROMATIC-HYDROCARBONS; VETERINARY DRUG RESIDUES; LIQUID-CHROMATOGRAPHY; POLYCHLORINATED-BIPHENYLS; MULTIRESIDUE ANALYSIS; QUANTITATIVE-ANALYSIS; FLOW-INJECTION; LC-MS/MS; MATRICES AB A new method of sample preparation was developed and is reported for the first time. The approach combines in-vial filtration with dispersive solid-phase extraction (d-SPE) in a fast and convenient cleanup of QuEChERS (quick, easy, cheap, effective, rugged, and safe) extracts. The method was applied to simultaneous analysis of 42 diverse pesticides and 17 environmental contaminants, including polycyclic aromatic hydrocarbons, polychlorinated biphenyls (PCBs), and flame retardants, in shrimp as the sample matrix. Final extracts were analyzed by both low-pressure gas chromatography - triple quadrupole tandem mass spectrometry (LPGC-MS/MS), and high-performance liquid chromatography - triple quadrupole tandem mass spectrometry (HPLC-MS/MS) to provide a wide scope of analysis for targeted analytes. During method development, several different commercial sorbents ford-SPE were investigated and compared with respect to analyte recoveries. The method was validated at 10, 50, and 100 ng g (1) spiking levels (10-fold lower for PCBs), and the results for nearly all analytes were between 70 and 115% recoveries with <= 17% relative standard deviations. The method was shown to be simple, fast, and effective for multi-application analysis of chemical residues in the representative food and environmental marker matrix. Published by Elsevier B.V. C1 [Han, Lijun] China Agr Univ, Coll Sci, Beijing 100094, Peoples R China. [Sapozhnikova, Yelena; Lehotay, Steven J.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Lehotay, SJ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. FU National Natural Science Foundation of China [21177155] FX We thank Sam Ellis of Thomson for the suggestion to investigate the filter-vial d-SPE approach. Jon Thompson of Applied Sciences provided CarbonX(R) and Olga Shimelis of Supelco provided Z-Sep sorbent for our study. Technical assistance of Tawana Simons and Alan Lightfield in the lab is much appreciated. Lijun Han's sabbatical funding was provided by the National Natural Science Foundation of China (No. 21177155). NR 27 TC 26 Z9 26 U1 3 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0003-2670 EI 1873-4324 J9 ANAL CHIM ACTA JI Anal. Chim. Acta PD MAY 27 PY 2014 VL 827 BP 40 EP 46 DI 10.1016/j.aca.2014.04.005 PG 7 WC Chemistry, Analytical SC Chemistry GA AH2BN UT WOS:000335925800006 PM 24832993 ER PT J AU Glenn, DM AF Glenn, D. Michael TI An analysis of ash and isotopic carbon discrimination (Delta C-13) methods to evaluate water use efficiency in apple SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus x domestica; Photosynthesis; Transpiration; Whole tree gas exchange; Yield ID MINERAL-CONTENT; PARTICLE FILM; GAS-EXCHANGE; EMPIRE APPLE; GRAIN-YIELD; WHEAT; PHOTOSYNTHESIS; AVAILABILITY; TEMPERATURE; IRRIGATION AB Apple cultivars are selected for fruit quality, disease and insect resistance, not water use efficiency (WUE), however, the need for more water use efficient crops is accelerating due to climate change and increased competition for water resources. WUE be calculated from short-term gas exchange measurements of photosynthesis (A) and transpiration (E-c) and expressed as A/E-c (instantaneous water use efficiency; WUEInst), or on a whole plant basis, measuring leaf, fruit and wood dry matter (DM) together with a measurement of water use (WU), and expressed as DM/WU. Plant ash and carbon isotope discrimination (Delta C-13) have been correlated with water use efficiency in herbaceous plants. The purpose of this study was to measure WUEInst and seasonal WUE of mature and productive apple trees in the field and evaluate WUE correlations with ash and Delta C-13. WUEInst was measured in whole tree gas exchange studies and from seasonal dry matter yield studies in 'Empire' apple for 2005, 2007 and 2009. Leaf ash and Delta C-13 were positively correlated with mean tree photosynthetic rate (A) and transpiration (E-c) but only leaf Delta C-13 was negatively correlated with WUEInst. In seasonal dry matter yield studies, leaf ash and Delta C-13 responses were highly negatively correlated with WUE based on the annual fruit yield/seasonal potential transpiration (E-f), termed WUEFrt. There were also highly significant correlations between leaf and shoot ash with leaf and shoot Delta C-13. Both leaf ash and Delta C-13 were useful in identifying extremes in apple WUE and leaf ash can be used at a much lower cost with a high through-put potential. Published by Elsevier B.V. C1 ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov NR 34 TC 3 Z9 3 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAY 26 PY 2014 VL 171 BP 32 EP 36 DI 10.1016/j.scienta.2014.03.031 PG 5 WC Horticulture SC Agriculture GA AJ7NC UT WOS:000337882400005 ER PT J AU White, K Pontius, J Schaberg, P AF White, Katharine Pontius, Jennifer Schaberg, Paul TI Remote sensing of spring phenology in northeastern forests: A comparison of methods, field metrics and sources of uncertainty SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; Green leaf phenology; Vegetation indices; Field to sensor scaling; EVI; NDVI ID DECIDUOUS BROADLEAF FOREST; LEAF-AREA INDEX; MODIS VEGETATION INDEXES; GROWING-SEASON LENGTH; TIME-SERIES; HEMISPHERICAL PHOTOGRAPHY; CANOPY STRUCTURE; SATELLITE DATA; CLIMATE-CHANGE; NEAR-SURFACE AB Current remote sensing studies of phenology have been limited to coarse spatial or temporal resolution and often lack a direct link to field measurements. To address this gap, we compared remote sensing methodologies using Landsat Thematic Mapper (TM) imagery to extensive field measurements in a mixed northern hardwood forest. Five vegetation indices, five mathematical fits to model a continuous temporal response, and a suite of threshold estimates for "start of spring/season" (SOS) assessments were compared to field measurements of bud burst stage and hemispherical photo derived canopy structural metrics (transparency, leaf area index, greenness). Results indicated that a four-parameter logistic model based on at least five spring coverages of the Enhanced Vegetation Index (EVI) and a SOS threshold of 0.3 was most closely related to field metrics and most accurate in predicting the date of full leaf out. Plot level SOS was predicted with a mean absolute error of 11 days for all species and elevation combinations, but improved to 9 days for hardwood dominated plots and 7 days for sugar maple dominated plots. Mean absolute error was improved to 8 days when forest type (mixed, conifer hardwood) was used to refine predictions. The consistency of prediction errors across forest types indicates that while overall accuracy across pixels may be low, inter-annual comparisons of changes in phenology on a pixel basis may provide accurate assessments of changes in phenology overtime. This was confirmed by application to seven years of independent phenology data predicted with 12 days of mean absolute error. However, image availability will be a limiting factor in areas of frequent cloud cover. (C) 2014 Elsevier Inc All rights reserved. C1 [White, Katharine; Pontius, Jennifer] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Pontius, Jennifer; Schaberg, Paul] US Forest Serv, USDA, No Res Stn, Burlington, VT 05405 USA. RP Pontius, J (reprint author), US Forest Serv, USDA, No Res Stn, 81 Carrigan Dr, Burlington, VT 05405 USA. EM kmwhite@uvm.edu; Jennifer.pontius@uvm.edu; pschaberg@fs.fed.us NR 58 TC 18 Z9 19 U1 11 U2 84 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAY 25 PY 2014 VL 148 BP 97 EP 107 DI 10.1016/j.rse.2014.03.017 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AI3PE UT WOS:000336773600008 ER PT J AU Fang, DD Jenkins, JN Deng, DD McCarty, JC Li, P Wu, JX AF Fang, David D. Jenkins, Johnie N. Deng, Dewayne D. McCarty, Jack C. Li, Ping Wu, Jixiang TI Quantitative trait loci analysis of fiber quality traits using a random-mated recombinant inbred population in Upland cotton (Gossypium hirsutum L.) SO BMC GENOMICS LA English DT Article DE Cotton; Fiber quality traits; Microsatellite markers; Quantitative trait loci; Random-mating; Recombinant inbred lines ID BACKCROSS-SELF APPROACH; BARBADENSE COTTON; QTL ANALYSIS; MOLECULAR DISSECTION; RIL POPULATION; INTERSPECIFIC VARIATION; LINKAGE DISEQUILIBRIUM; GENETIC DIVERSITY; YIELD COMPONENTS; GERMPLASM AB Background: Upland cotton (Gossypium hirsutum L.) accounts for about 95% of world cotton production. Improving Upland cotton cultivars has been the focus of world-wide cotton breeding programs. Negative correlation between yield and fiber quality is an obstacle for cotton improvement. Random-mating provides a potential methodology to break this correlation. The suite of fiber quality traits that affect the yarn quality includes the length, strength, maturity, fineness, elongation, uniformity and color. Identification of stable fiber quantitative trait loci (QTL) in Upland cotton is essential in order to improve cotton cultivars with superior quality using marker-assisted selection (MAS) strategy. Results: Using 11 diverse Upland cotton cultivars as parents, a random-mated recombinant inbred (RI) population consisting of 550 RI lines was developed after 6 cycles of random-mating and 6 generations of self-pollination. The 550 RILs were planted in triplicates for two years in Mississippi State, MS, USA to obtain fiber quality data. After screening 15538 simple sequence repeat (SSR) markers, 2132 were polymorphic among the 11 parents. One thousand five hundred eighty-two markers covering 83% of cotton genome were used to genotype 275 RILs (Set 1). The marker-trait associations were analyzed using the software program TASSEL. At p < 0.01, 131 fiber QTLs and 37 QTL clusters were identified. These QTLs were responsible for the combined phenotypic variance ranging from 62.3% for short fiber content to 82.8% for elongation. The other 275 RILs (Set 2) were analyzed using a subset of 270 SSR markers, and the QTLs were confirmed. Two major QTL clusters were observed on chromosomes 7 and 16. Comparison of these 131 QTLs with the previously published QTLs indicated that 77 were identified before, and 54 appeared novel. Conclusions: The 11 parents used in this study represent a diverse genetic pool of the US cultivated cotton, and 10 of them were elite commercial cultivars. The fiber QTLs, especially QTL clusters reported herein can be readily implemented in a cotton breeding program to improve fiber quality via MAS strategy. The consensus QTL regions warrant further investigation to better understand the genetics and molecular mechanisms underlying fiber development. C1 [Fang, David D.; Li, Ping] ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, New Orleans, LA 70124 USA. [Jenkins, Johnie N.; Deng, Dewayne D.; McCarty, Jack C.] ARS, Genet & Precis Agr Res Unit, USDA, Mississippi State, MS 39762 USA. [Wu, Jixiang] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. RP Fang, DD (reprint author), ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, New Orleans, LA 70124 USA. EM David.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU United States Department of Agriculture-Agricultural Research Service CRIS [6435-21000-017-00D]; Cotton Incorporated project [10-747] FX This research was funded by United States Department of Agriculture-Agricultural Research Service CRIS project 6435-21000-017-00D and Cotton Incorporated project 10-747. We thank Dr. Russell Hayes for assisting with the field experiments, Dr. Gregory Thyssen for his assistance to align marker sequences against the G. raimondii reference genome. We also thank Drs. Kater Hake and Don Jones from Cotton Incorporated for their support from the onset of this project. Our appreciation goes to Mrs. Sheron Simpson and Dr. Brian Scheffler at the Genomics and Bioinformatics Research Unit at Stoneville, MS for their excellent support in SSR marker analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture which is an equal opportunity provider and employer. NR 65 TC 21 Z9 22 U1 0 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 24 PY 2014 VL 15 AR 397 DI 10.1186/1471-2164-15-397 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AI5PR UT WOS:000336922000001 PM 24886099 ER PT J AU Carrero-Colon, M Abshire, N Sweeney, D Gaskin, E Hudson, K AF Carrero-Colon, Militza Abshire, Nathan Sweeney, Daniel Gaskin, Erik Hudson, Karen TI Mutations in SACPD-C Result in a Range of Elevated Stearic Acid Concentration in Soybean Seed SO PLOS ONE LA English DT Article ID CARRIER-PROTEIN-DESATURASE; OIL; GENE AB Soybean oil has a wide variety of uses, and stearic acid, which is a relatively minor component of soybean oil is increasingly desired for both industrial and food applications. New soybean mutants containing high levels of the saturated fatty acid stearate in seeds were recently identified from a chemically mutagenized population. Six mutants ranged in stearate content from 6-14% stearic acid, which is 1.5 to 3 times the levels contained in wild-type seed of the Williams 82 cultivar. Candidate gene sequencing revealed that all of these lines carried amino acid substitutions in the gene encoding the delta-9-stearoyl-acyl-carrier protein desaturase enzyme (SACPD-C) required for the conversion of stearic acid to oleic acid. Five of these missense mutations were in highly conserved residues clustered around the predicted di-iron center of the SACPD-C enzyme. Co-segregation analysis demonstrated a positive association of the elevated stearate trait with the SACPD-C mutation for three populations. These missense mutations may provide additional alleles that may be used in the development of new soybean cultivars with increased levels of stearic acid. C1 [Carrero-Colon, Militza; Hudson, Karen] ARS, Crop Prod & Pest Control Res Unit, USDA, W Lafayette, IN 47905 USA. [Abshire, Nathan; Sweeney, Daniel; Gaskin, Erik] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Hudson, K (reprint author), ARS, Crop Prod & Pest Control Res Unit, USDA, W Lafayette, IN 47905 USA. EM hudson@ars.usda.gov FU USDA-ARS Current Research Information System [3602-21000-006-00D] FX Funding for this work was provided through the USDA-ARS Current Research Information System 3602-21000-006-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 17 TC 2 Z9 2 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2014 VL 9 IS 5 AR e97891 DI 10.1371/journal.pone.0097891 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM0UW UT WOS:000339563400070 PM 24846334 ER PT J AU Guo, QF Olden, JD AF Guo, Qinfeng Olden, Julian D. TI Spatial Scaling of Non-Native Fish Richness across the United States SO PLOS ONE LA English DT Article ID FRESH-WATER FISHES; SPECIES RICHNESS; HUMAN-POPULATION; INVASIBILITY; DIVERSITY; BIODIVERSITY; COMMUNITY; INVASION; HOMOGENIZATION; PATTERNS AB A major goal and challenge of invasion ecology is to describe and interpret spatial and temporal patterns of species invasions. Here, we examined fish invasion patterns at four spatially structured and hierarchically nested scales across the contiguous United States (i.e., from large to small: region, basin, watershed, and sub-watershed). All spatial relationships in both richness and fraction between species groups (e.g., natives vs. exotics) were positive at large scales. However, contrary to predictions using null/neutral models, the patterns at small scales were hump-shaped (unimodal), not simply negative. The fractions of both domestic (introduced among watersheds within the USA) and foreign (introduced from abroad) exotics increased with area across scales but decreased within each scale. The foreign exotics exhibited the highest dominance (lowest evenness) and spatial variation in distribution, followed by domestic exotics and natives, although on average natives still occupy larger areas than domestic and foreign exotics. The results provide new insight into patterns and mechanisms of fish species invasions at multiple spatial scales in the United States. C1 [Guo, Qinfeng] USDA FS, Eastern Forest Environm Threat Assessment Ctr, Asheville, NC 28804 USA. [Olden, Julian D.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. RP Guo, QF (reprint author), USDA FS, Eastern Forest Environm Threat Assessment Ctr, Asheville, NC 28804 USA. EM qguo@fs.fed.us OI Olden, Julian/0000-0003-2143-1187; Higgs, Dennis/0000-0002-0771-4642 FU University of Washington FX We thank S. Fei and B. Iannone for helpful discussions on related topics, two anonymous reviewers for comments on the manuscript, J. Wen for assistance with data analyses, and W. Christie for assistance with GIS. Support to JDO was provided in part by the University of Washington H. Mason Keeler Endowed Professorship. NR 39 TC 1 Z9 1 U1 2 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2014 VL 9 IS 5 AR e97727 DI 10.1371/journal.pone.0097727 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM0UW UT WOS:000339563400051 PM 24844226 ER PT J AU Hill, JM Egan, JF Stauffer, GE Diefenbach, DR AF Hill, Jason M. Egan, J. Franklin Stauffer, Glenn E. Diefenbach, Duane R. TI Habitat Availability Is a More Plausible Explanation than Insecticide Acute Toxicity for US Grassland Bird Species Declines SO PLOS ONE LA English DT Article ID LAND-USE; BREEDING BIRDS; FARMLAND BIRDS; UNITED-STATES; AGRICULTURAL LANDSCAPES; PATCH SIZE; COUNT DATA; CONSERVATION; POPULATIONS; MANAGEMENT AB Grassland bird species have experienced substantial declines in North America. These declines have been largely attributed to habitat loss and degradation, especially from agricultural practices and intensification (the habitat-availability hypothesis). A recent analysis of North American Breeding Bird Survey (BBS) "grassland breeding'' bird trends reported the surprising conclusion that insecticide acute toxicity was a better correlate of grassland bird declines in North America from 1980-2003 (the insecticide-acute-toxicity hypothesis) than was habitat loss through agricultural intensification. In this paper we reached the opposite conclusion. We used an alternative statistical approach with additional habitat covariates to analyze the same grassland bird trends over the same time frame. Grassland bird trends were positively associated with increases in area of Conservation Reserve Program (CRP) lands and cropland used as pasture, whereas the effect of insecticide acute toxicity on bird trends was uncertain. Our models suggested that acute insecticide risk potentially has a detrimental effect on grassland bird trends, but models representing the habitat-availability hypothesis were 1.3-21.0 times better supported than models representing the insecticide-acute-toxicity hypothesis. Based on point estimates of effect sizes, CRP area and agricultural intensification had approximately 3.6 and 1.6 times more effect on grassland bird trends than lethal insecticide risk, respectively. Our findings suggest that preserving remaining grasslands is crucial to conserving grassland bird populations. The amount of grassland that has been lost in North America since 1980 is well documented, continuing, and staggering whereas insecticide use greatly declined prior to the 1990s. Grassland birds will likely benefit from the de-intensification of agricultural practices and the interspersion of pastures, Conservation Reserve Program lands, rangelands and other grassland habitats into existing agricultural landscapes. C1 [Hill, Jason M.; Stauffer, Glenn E.] Penn State Univ, Penn Cooperat Fish & Wildlife Res Unit, University Pk, PA 16802 USA. [Egan, J. Franklin] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Diefenbach, Duane R.] Penn State Univ, Penn Cooperat Fish & Wildlife Res Unit, US Geol Survey, University Pk, PA 16802 USA. RP Hill, JM (reprint author), Penn State Univ, Penn Cooperat Fish & Wildlife Res Unit, University Pk, PA 16802 USA. EM jmh656@psu.edu NR 82 TC 5 Z9 5 U1 5 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 20 PY 2014 VL 9 IS 5 AR e98064 DI 10.1371/journal.pone.0098064 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM0UW UT WOS:000339563400090 PM 24846309 ER PT J AU Silva, CJ Erickson-Beltran, ML Skinner, CB Dynin, I Hui, C Patfield, SA Carter, JM He, XH AF Silva, Christopher J. Erickson-Beltran, Melissa L. Skinner, Craig B. Dynin, Irina Hui, Colleen Patfield, Stephanie A. Carter, John Mark He, Xiaohua TI Safe and Effective Means of Detecting and Quantitating Shiga-Like Toxins in Attomole Amounts SO ANALYTICAL CHEMISTRY LA English DT Article ID IONIZATION MASS-SPECTROMETRY; ENTEROHEMORRHAGIC ESCHERICHIA-COLI; HEMOLYTIC-UREMIC SYNDROME; RELATIVE TOXICITIES; PRION PROTEIN; PURIFICATION; STRAINS; CELLS; ASSAY; PCR AB Shiga-like toxins (verotoxins) are a class of AB(5) holotoxins that are primarily responsible for the virulence associated with Shiga-like toxin producing Escherichia coli (STEC) infections. The holotoxins are composed of a pentamer of identical subunits (B subunit) responsible for delivering the catalytic subunit (A subunit) to a host cell and facilitating endocytosis of the toxin into the cell. The B subunits are not associated with toxicity. We developed a multiple reaction monitoring method based on analyzing conserved peptides, derived from the tryptic digestion of the B subunits. Stable-isotope-labeled analogues were prepared and used as internal standards to identify and quantify these characteristic peptides. We were able to detect and quantify Shiga toxins (Stx), Shiga-like toxin type 1 (Stxl) and type 2 (Stx2) subtypes, and to distinguish among most of the known subtypes. The limit of detection for digested pure standards was in the low attomole range/injection (similar to 10 attomoles), which corresponded to a concentration of 1.7 femtomol/mL. A matrix effect was observed when dilute samples were digested in the buffer, Luria broth, or mouse plasma (LOD similar to 30 attomol/injection = 5 femtomol/mL). In addition, we determined that the procedures necessary to perform our mass spectrometry-based analysis completely inactivate the toxins present in the sample. This is a safe and effective method of detecting and quantitating Stx, Stxl, and Stx2, since it does not require the use of intact toxins. C1 [Silva, Christopher J.; Erickson-Beltran, Melissa L.; Skinner, Craig B.; Dynin, Irina; Hui, Colleen; Patfield, Stephanie A.; Carter, John Mark; He, Xiaohua] USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Silva, CJ (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94706 USA. EM christopher.silva@ars.usda.gov RI Carter, John Mark/K-2485-2015; OI Carter, John Mark/0000-0001-8251-4168; Silva, Christopher/0000-0003-4521-6377 NR 55 TC 3 Z9 3 U1 0 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD MAY 20 PY 2014 VL 86 IS 10 BP 4698 EP 4706 DI 10.1021/ac402930r PG 9 WC Chemistry, Analytical SC Chemistry GA AH8WJ UT WOS:000336418600011 PM 24761992 ER PT J AU Sun, X Liu, Z Zhang, AJ Dong, HB Zeng, FF Pan, XY Wang, YM Wang, MQ AF Sun, Xiao Liu, Zhuang Zhang, Aijun Dong, Hai-Bo Zeng, Fang-Fang Pan, Xiang-Yu Wang, Yongmo Wang, Man-Qun TI Electrophysiological responses of the rice leaffolder, Cnaphalocrocis medinalis, to rice plant volatiles SO JOURNAL OF INSECT SCIENCE LA English DT Article DE electroantennogram ID SYNTHETIC PHEROMONE BLENDS; FEMALE SEX-PHEROMONE; ELECTROANTENNOGRAM RESPONSES; LYGUS-HESPERUS; GRAPEVINE MOTH; HOST-PLANT; GEOGRAPHIC VARIATIONS; BEHAVIORAL-RESPONSES; HEADSPACE VOLATILES; MATING STATUS AB The rice leaffolder, Cnaphalocrocis medinalis Guenee (Lepidoptera: Pyralidae), is one of the most destructive pests of rice. Electrophysiological responses of this species to 38 synthetic volatiles known to be released from rice plants (Poaceae: Oryza spp.) were studied using the electroantennogram (EAG) method. Compounds that elicited the strongest EAG responses for each physiological condition were selected for EAG dose-response tests at five concentrations. These compounds included: methyl salicylate, heptanol, linalool, cyclohexanol, and 2-heptanone for one-day-old male moths; heptanol, hexanal, (Z)-2-hexen-1-ol, and nonadecane for one-day-old females; methyl salicylate, heptanol, (E)-2-hexen-1-ol, and (Z)-2-hexen-1-ol for three-day-old males; linalool, heptanol, (E)-2-hexen-1-ol, 2-heptanone, and hexanal for three-day-old females; 2-heptanone, cyclohexanol, linalool, heptanol, and methyl salicylate for five-day- old virgin females; and methyl benzoate, (Z)-2-hexen-1-ol, heptanol, linalool, and hexanal for five-day- old mated females. Female and male C. medinalis exhibited broad overlap in their EAG responses, and there was no clear difference between male and female EAG responses to different compounds. Statistical analyses revealed that both volatile compound chemical structure and C. medinalis physiological condition (age, sex, and mating condition) had an effect on EAG response. C1 [Sun, Xiao; Liu, Zhuang; Dong, Hai-Bo; Zeng, Fang-Fang; Pan, Xiang-Yu; Wang, Yongmo; Wang, Man-Qun] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. [Zhang, Aijun] USDA ARS, Inst Plant Sci, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Sun, X (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Peoples R China. EM sunxiao19860310@126.com; 294231362@qq.com; zhanga@ba.ars.usda.gov; donghaibonihao@163.com; nl19882006@163.com; kingchys_pan@126.com; ymwang@mail.hzau.edu.cn; mqwang@mail.hzau.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest of China [201403030]; Huazhong Agricultural University Scientific & Technological Self-innovation Foundation; Industry Project of Ministry of Agriculture of China [200903051] FX We acknowledge technical assistance from Mr. J-M Zhang of Zhejiang Academy of Agricultural Sciences with EAG experiments. This study was supported and funded by the Special Fund for Agro-scientific Research in the Public Interest of China (201403030), Huazhong Agricultural University Scientific & Technological Self-innovation Foundation, and The Industry Project of Ministry of Agriculture of China (200903051). NR 59 TC 4 Z9 5 U1 6 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD MAY 20 PY 2014 VL 14 AR 70 PG 14 WC Entomology SC Entomology GA AH6MG UT WOS:000336244400002 PM 25373217 ER PT J AU Beirn, LA Clarke, BB Crouch, JA AF Beirn, Lisa A. Clarke, Bruce B. Crouch, Jo Anne TI Influence of Host and Geographic Locale on the Distribution of Colletotrichum cereale Lineages SO PLOS ONE LA English DT Article ID BLUEGRASS PUTTING GREEN; ANTHRACNOSE DISEASE; CALIFORNIA POPULATIONS; TURFGRASS; PATHOGEN; GRAMINICOLA; SEVERITY; GRASSES; GENOME AB Colletotrichum cereale is an ascomycete inhabitant of cool-season Pooideae grasses. The fungus has increased in frequency over the past decade as a destructive pathogen of Poa annua and Agrostis stolonifera turfgrass. Colletotrichum cereale exists as two lineages, designated clades A and B, but little is known about the distribution of these clades in natural environments, or what role these subdivisions may play in the trajectory of disease outbreaks. In this study, our objective was to determine the frequency of C. cereale clades A and B. To rapidly discriminate between the two C. cereale clades, a real-time PCR assay was developed based on the Apn2 gene. A collection of 700 C. cereale pathogens and endophytes from twenty Pooideae grass genera were genotyped. 87% of the collection was identifed as part of clade A, 11.7% as part of clade B, and 1.3% was a mixture. Colletotrichum cereale from turfgrass hosts in North America were most commonly members of clade A (78%). The overabundance of clade A in turfgrass isolates was directly attributable to the dominance of this lineage from southern sampling sites, irrespective of host. In contrast, 111 C. cereale turfgrass isolates collected from northern sampling sites were evenly distributed between clades A and B. Only 28% of C. cereale from A. stolonifera at northern sampling sites were part of clade A. These data show that environmental factors such as geographic location and host identity likely played a role in the distribution of the major C. cereale clades in North American turfgrass. C1 [Beirn, Lisa A.; Clarke, Bruce B.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ USA. [Crouch, Jo Anne] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM joanne.crouch@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090 FU Rutgers Center for Turfgrass Science; U.S. Department of Agriculture (USDA) Agriculture Research Service; USDA National Institute of Food and Agriculture FX This work was funded by the Rutgers Center for Turfgrass Science, the U.S. Department of Agriculture (USDA) Agriculture Research Service, and the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 3 Z9 3 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2014 VL 9 IS 5 AR e97706 DI 10.1371/journal.pone.0097706 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN9SK UT WOS:000340948600060 PM 24842654 ER PT J AU Fabrick, JA Ponnuraj, J Singh, A Tanwar, RK Unnithan, GC Yelich, AJ Li, XC Carriere, Y Tabashnik, BE AF Fabrick, Jeffrey A. Ponnuraj, Jeyakumar Singh, Amar Tanwar, Raj K. Unnithan, Gopalan C. Yelich, Alex J. Li, Xianchun Carriere, Yves Tabashnik, Bruce E. TI Alternative Splicing and Highly Variable Cadherin Transcripts Associated with Field-Evolved Resistance of Pink Bollworm to Bt Cotton in India SO PLOS ONE LA English DT Article ID BACILLUS-THURINGIENSIS TOXIN; ARMIGERA LEPIDOPTERA-NOCTUIDAE; EUROPEAN CORN-BORER; HELICOVERPA-ARMIGERA; INSECT RESISTANCE; TRANSGENIC COTTON; PECTINOPHORA-GOSSYPIELLA; HELIOTHIS-VIRESCENS; CROSS-RESISTANCE; CONFERRING RESISTANCE AB Evolution of resistance by insect pests can reduce the benefits of insecticidal proteins from Bacillus thuringiensis (Bt) that are used extensively in sprays and transgenic crops. Despite considerable knowledge of the genes conferring insect resistance to Bt toxins in laboratory-selected strains and in field populations exposed to Bt sprays, understanding of the genetic basis of field-evolved resistance to Bt crops remains limited. In particular, previous work has not identified the genes conferring resistance in any cases where field-evolved resistance has reduced the efficacy of a Bt crop. Here we report that mutations in a gene encoding a cadherin protein that binds Bt toxin Cry1Ac are associated with field-evolved resistance of pink bollworm (Pectinophora gossypiella) in India to Cry1Ac produced by transgenic cotton. We conducted laboratory bioassays that confirmed previously reported resistance to Cry1Ac in pink bollworm from the state of Gujarat, where Bt cotton producing Cry1Ac has been grown extensively. Analysis of DNA from 436 pink bollworm from seven populations in India detected none of the four cadherin resistance alleles previously reported to be linked with resistance to Cry1Ac in laboratory-selected strains of pink bollworm from Arizona. However, DNA sequencing of pink bollworm derived from resistant and susceptible field populations in India revealed eight novel, severely disrupted cadherin alleles associated with resistance to Cry1Ac. For these eight alleles, analysis of complementary DNA (cDNA) revealed a total of 19 transcript isoforms, each containing a premature stop codon, a deletion of at least 99 base pairs, or both. Seven of the eight disrupted alleles each produced two or more different transcript isoforms, which implicates alternative splicing of messenger RNA (mRNA). This represents the first example of alternative splicing associated with field-evolved resistance that reduced the efficacy of a Bt crop. C1 [Fabrick, Jeffrey A.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. [Ponnuraj, Jeyakumar] Natl Inst Plant Hlth Management, Hyderabad, Andhra Pradesh, India. [Singh, Amar; Tanwar, Raj K.] Indian Agr Res Inst, Natl Ctr Integrated Pest Management, Delhi, India. [Unnithan, Gopalan C.; Yelich, Alex J.; Li, Xianchun; Carriere, Yves; Tabashnik, Bruce E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. RP Fabrick, JA (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. EM jeff.fabrick@ars.usda.gov FU U.S. Department of Agriculture [5347-22620-021-00D]; U.S. Department of Agriculture Agriculture and Food Research Initiative [2008-35302-0390]; Biotechnology Risk Assessment Research Grants Program [2011-33522-30729]; Department of Science and Technology (India) Better Opportunities for Young Scientists in Chosen Areas of Science and Technology (BOYSCAST) fellowship FX This work was funded by U.S. Department of Agriculture base funding (Project Number 5347-22620-021-00D) to J.A.F., research grants from the U.S. Department of Agriculture Agriculture and Food Research Initiative (2008-35302-0390) and Biotechnology Risk Assessment Research Grants Program (2011-33522-30729) to B. E. T., and the Department of Science and Technology (India) Better Opportunities for Young Scientists in Chosen Areas of Science and Technology (BOYSCAST) fellowship to J.P. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 84 TC 33 Z9 37 U1 3 U2 36 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2014 VL 9 IS 5 AR e97900 DI 10.1371/journal.pone.0097900 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN9SK UT WOS:000340948600089 PM 24840729 ER PT J AU Laughery, JM Knowles, DP Schneider, DA Bastos, RG McElwain, TF Suarez, CE AF Laughery, Jacob M. Knowles, Donald P. Schneider, David A. Bastos, Reginaldo G. McElwain, Terry F. Suarez, Carlos E. TI Targeted Surface Expression of an Exogenous Antigen in Stably Transfected Babesia bovis SO PLOS ONE LA English DT Article ID TICK BOOPHILUS-MICROPLUS; REACTIVE ANTIBODIES; INVITRO CULTIVATION; VACCINE GAVAC(TM); CATTLE; PROTEIN; GENE; BM86; COLOCALIZATION; IMMUNODOMINANT AB Babesia bovis is a tick-borne intraerythocytic protozoan responsible for acute disease in cattle which can be controlled by vaccination with attenuated B. bovis strains. Emerging B. bovis transfection technologies may increase the usefulness of these live vaccines. One use of transfected B. bovis parasites may be as a vaccine delivery platform. Previous transfection methods for B. bovis were limited by single expression sites and intracellular expression of transfected antigens. This study describes a novel transfection system in which two exogenous genes are expressed: one for selection and the other for a selected antigen designed to be delivered to the surface of the parasites. The strategy for duplicating the number of transfected genes was based on the use of the putative bidirectional promoter of the B. bovis 1.4 Kb ef-1 alpha intergenic region. The ability of this region to regulate two independent expression sites was demonstrated using a luciferase assay on transiently transfected B. bovis parasites and then incorporated into a stable transfection plasmid to control independent expression of the selectable marker GFP-BSD and another gene of interest. A chimeric gene was synthetized using sequences from the protective B-cell epitopes of Rhipicephalus microplus tick antigen Bm86 along with sequences from the surface exposed B. bovis major surface antigen-1. This chimeric gene was then cloned into the additional expression site of the transfection plasmid. Transfection of the B. bovis Mo7 strain with this plasmid resulted in stable insertion into the ef-1 alpha locus and simultaneous expression of both exogenous genes. Expression of the Bm86 epitopes on the surface of transfected merozoites was demonstrated using immunofluorescence analyses. The ability to independently express multiple genes by the inclusion of a bidirectional promoter and the achievement of surface expression of foreign epitopes advances the potential of transfected B. bovis as a future vaccine delivery platform. C1 [Laughery, Jacob M.; Knowles, Donald P.; Bastos, Reginaldo G.; McElwain, Terry F.; Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Knowles, Donald P.; Schneider, David A.; Suarez, Carlos E.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [McElwain, Terry F.] Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Suarez, CE (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. EM ces@vetmed.wsu.edu FU United States Department of Agriculture-Agriculture Research Service Current Research Information System Project [5348-32000-028-00D]; United States Agency for International Development Grant [PCE-G-0098-00043-00]; USDA [SCA58-5348-7-528, 5348-32000-028-055] FX This work was supported by United States Department of Agriculture-Agriculture Research Service Current Research Information System Project No. 5348-32000-028-00D, United States Agency for International Development Grant PCE-G-0098-00043-00, and USDA (SCA58-5348-7-528) agreement 5348-32000-028-055. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 7 Z9 7 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 19 PY 2014 VL 9 IS 5 AR e97890 DI 10.1371/journal.pone.0097890 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN9SK UT WOS:000340948600087 PM 24840336 ER PT J AU Arsenault, RJ Maattanen, P Daigle, J Potter, A Griebel, P Napper, S AF Arsenault, Ryan J. Maattanen, Pekka Daigle, Joanna Potter, Andrew Griebel, Philip Napper, Scott TI From mouth to macrophage: mechanisms of innate immune subversion by Mycobacterium avium subsp paratuberculosis SO VETERINARY RESEARCH LA English DT Review ID TOLL-LIKE RECEPTORS; MONOCYTE-DERIVED MACROPHAGES; ACTIVATED PROTEIN-KINASE; HOST-CELL APOPTOSIS; IFN-GAMMA RECEPTOR; VACUOLAR-H+-ATPASE; GENE-EXPRESSION; BOVINE MONOCYTES; INTERFERON-GAMMA; MONONUCLEAR PHAGOCYTES AB Johne's disease (JD) is a chronic enteric infection of cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP). The high economic cost and potential zoonotic threat of JD have driven efforts to develop tools and approaches to effectively manage this disease within livestock herds. Efforts to control JD through traditional animal management practices are complicated by MAP's ability to cause long-term environmental contamination as well as difficulties associated with diagnosis of JD in the pre-clinical stages. As such, there is particular emphasis on the development of an effective vaccine. This is a daunting challenge, in large part due to MAP's ability to subvert protective host immune responses. Accordingly, there is a priority to understand MAP's interaction with the bovine host: this may inform rational targets and approaches for therapeutic intervention. Here we review the early host defenses encountered by MAP and the strategies employed by the pathogen to avert or subvert these responses, during the critical period between ingestion and the establishment of persistent infection in macrophages. C1 [Arsenault, Ryan J.] ARS, USDA, SPARC, College Stn, TX 77845 USA. [Maattanen, Pekka; Daigle, Joanna; Potter, Andrew; Griebel, Philip; Napper, Scott] Univ Saskatchewan, VIDO InterVac, Saskatoon, SK S7N 5E3, Canada. [Daigle, Joanna; Napper, Scott] Univ Saskatchewan, Dept Biochem, Saskatoon, SK S7N 5E5, Canada. [Griebel, Philip] Univ Saskatchewan, Sch Publ Hlth, Saskatoon, SK S7N 5E5, Canada. RP Napper, S (reprint author), Univ Saskatchewan, VIDO InterVac, Saskatoon, SK S7N 5E3, Canada. EM scott.napper@usask.ca NR 100 TC 11 Z9 11 U1 4 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD MAY 15 PY 2014 VL 45 AR 54 DI 10.1186/1297-9716-45-54 PG 15 WC Veterinary Sciences SC Veterinary Sciences GA AI5SF UT WOS:000336928700001 PM 24885748 ER PT J AU Fageria, NK Baligar, VC AF Fageria, N. K. Baligar, V. C. TI Macronutrient-Use Efficiency and Changes in Chemical Properties of an Oxisol as Influenced by Phosphorus Fertilization and Tropical Cover Crops SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE Concentration of macro and micronutrients; Oxisol; shoot dry weight; soil chemical properties ID UPPER MIDWEST; SOIL ACIDITY; RYE; ROTATION; CORN AB Cover crops are important components of copping systems due to their beneficial effects on soil physical, chemical, and biological properties. A greenhouse experiment was conducted to evaluate influence of phosphorus (P) fertilization on nutrient-use efficiency of 14 tropical cover crops. The P levels tested were 0 (low), 100 (medium), and 200 (high) mg kg(-1) of soil. The cover crops tested were Crotalaria breviflora, Crotalaria breviflora, Crotalaria spectabilis Roth, Crotalaria ochroleuca G. Don, Crotalaria juncea L., Crotalaria mucronata, Calapogonium mucunoides, Pueraria phaseoloides Roxb., Pueraria phaseoloides Roxb., Cajanus cajan L. Millspaugh, Dolichos lablab L., Mucuna deeringiana (Bort) Merr., Mucuna cinereum L., and Canavalia ensiformis L. DC. Agronomic efficiency (shoot dry weight per unit P applied), physiological efficiency (shoot dry weight per unit of nutrient uptake), and apparent recovery efficiency (nutrient uptake in the shoot per unit nutrient applied) were significantly varied among cover crops. Agronomic efficiency decreased with increasing P levels. Overall, physiological efficiency of nutrient uptake was in the order of P > sulfur (S) > magnesium (Mg) > calcium (Ca) > potassium (K) > nitrogen (N). Similarly, apparent recovery efficiency was in the order of N > K > Ca > Mg > P > S. Different recovery efficiency in cover crops can be useful in selecting cover crops with high recovery efficiency, which may be beneficial to succeeding crops in the cropping systems. The P x cover crops interactions were significant for soil extractable Ca2+, P, cation exchange capacity (CEC), Ca saturation, Ca/K ratio, and K/Mg ratio, indicating that cover crops change these soil property differently under different P levels. Thus, cover crops selection for different P levels is an important strategy for using cover crops in cropping systems in Brazilian Oxisols. Optimal values of soil pH, soil Ca and Mg contents, hydrogen (H) + aluminum (Al), P, CEC, base saturation, Ca saturation, Mg saturation, and K saturation were established for tropical cover crops grown on an Oxisol. C1 [Fageria, N. K.] Natl Rice & Bean Res Ctr EMBRAPA, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Baligar, V. C.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Fageria, NK (reprint author), Natl Rice & Bean Res Ctr EMBRAPA, Caixa Postal 179, BR-75375000 Santo Antonio De Goias, Go, Brazil. EM nand.fageria@.embrapa.br NR 22 TC 0 Z9 0 U1 2 U2 18 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 520 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0010-3624 EI 1532-2416 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD MAY 15 PY 2014 VL 45 IS 9 BP 1227 EP 1246 DI 10.1080/00103624.2013.874030 PG 20 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA AG1YQ UT WOS:000335212500005 ER PT J AU Knapp, BO Walker, JL Wang, GG Hu, HF Addington, RN AF Knapp, Benjamin O. Walker, Joan L. Wang, G. Geoff Hu, Huifeng Addington, Robert N. TI Effects of overstory retention, herbicides, and fertilization on sub-canopy vegetation structure and functional group composition in loblolly pine forests restored to longleaf pine SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Canopy retention; Ecological restoration; Fertilization; Herbicide; Mid-story density ID PALUSTRIS MILL. SEEDLINGS; HARDWOOD REDUCTION TECHNIQUES; TAEDA L. STANDS; HEXAZINONE APPLICATION; UNDERSTORY VEGETATION; WIREGRASS ECOSYSTEMS; NORTHWEST FLORIDA; SPECIES-DIVERSITY; SITE PREPARATION; PRESCRIBED FIRE AB The desirable structure of longleaf pine forests, which generally includes a relatively open canopy of pines, very few woody stems in the mid-story, and a well-developed, herbaceous ground layer, provides critical habitat for flora and fauna and contributes to ecosystem function. Current efforts to restore longleaf pine to upland sites dominated by second-growth loblolly pine require information about how restoration treatments affect sub-canopy vegetation. We established a field experiment at Fort Benning in Georgia and Alabama, USA to determine the effects of four levels of approximately uniform canopy density (Control [similar to 16 m(2)/ha basal area], MedBA [similar to 9 m(2)/ha basal area], LowBA [similar to 5 m(2)/ha basal area], and Clearcut [0 m(2)/ha basal area]) and three cultural treatments (NT [untreated], H [chemical control of woody and herbaceous vegetation] and H + F [chemical control plus fertilization]) on vegetation structure and functional group composition for three growing seasons following canopy removal. In general, cover (a measure of abundance) of ground layer vegetation increased with the amount of canopy removal. The ground layer was dominated by herbaceous vegetation in each year. Canopy trees generally suppressed the cover of graminoids in the first two years after treatment but only the Control plots had lower graminoid cover than Clearcut plots after the third growing season. Forb cover was significantly lower on Control plots than on Clearcut plots after only the first growing season, and woody stems/shrubs had lower cover on Control plots than on LowBA or Clearcut plots in each year. Vegetation cover increased following the first year after canopy removal, and the relative dominance of functional groups did not change through time. Canopy retention limited the development of mid-story woody stems, with the greatest stem densities in the Clearcut plots. The herbicide treatment (on both H and H + F) significantly reduced woody stem density in the mid-story in 2009, but the effect was no longer significant in 2010. Traditional methods for converting stands of other pine species to longleaf pine commonly include clearcutting followed by planting, but our results suggest that clearcutting may release woody vegetation to increase mid-story stem densities and will reduce the amount of pine needles in the fuel bed. Retaining low to moderate levels of canopy density (5-9 m(2)/ha basal area) in loblolly pine stands may provide an effective balance for reaching multiple restoration objectives that include maintaining desirable vegetation structure and creating fuel conditions for a frequent fire regime. (C) 2014 Elsevier B.V. All rights reserved. C1 [Knapp, Benjamin O.; Wang, G. Geoff; Hu, Huifeng] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Walker, Joan L.] Clemson Univ, USDA, Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. [Addington, Robert N.] Nature Conservancy, Ft Benning, GA 31995 USA. RP Knapp, BO (reprint author), Univ Missouri, Dept Forestry, 203-S ABNR Bldg, Columbia, MO 65211 USA. EM knappb@missouri.edu FU Strategic Environmental Research and Development Program (SERDP) [RC-1474]; United States Department of Defense, United States Department of Energy; United States Environmental Protection Agency FX Funding for this research was provided by the Strategic Environmental Research and Development Program (SERDP; Project RC-1474), sponsored by the United States Department of Defense, United States Department of Energy, and United States Environmental Protection Agency. We would like to thank the personnel of the Land Management Branch at Fort Benning, with special recognition of Robert Larimore, James Parker, and Stephen Hudson. We are grateful for the support of The Nature Conservancy at Fort Benning - Geoff Sorrell, Michele Elmore, and Wade Harrison. Finally, this research could not have been accomplished without the assistance of numerous field technicians, including Bryan Mudder, Erik Pearson, Hunter Leary, Carsyn Tennant, Evelyn Wenk, and Seth Cook. Statistical advice was provided by Dr. James Rieck of Clemson University. This paper is technical contribution number 6239 of the Clemson University Experiment Station. NR 68 TC 3 Z9 3 U1 1 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 15 PY 2014 VL 320 BP 149 EP 160 DI 10.1016/j.foreco.2014.03.021 PG 12 WC Forestry SC Forestry GA AH4PN UT WOS:000336110600016 ER PT J AU Levings, RL Stoll, IR Warg, JV Patterson, PA Hobbs, LA Kaeberle, ML Roth, JA AF Levings, Randall L. Stoll, Ione R. Warg, Janet V. Patterson, Peggy A. Hobbs, Lea Ann Kaeberle, Merlin L. Roth, James A. TI Generation by self re-fusion of bovine(3) x murine(2) heterohybridomas secreting virus-neutralizing bovine monoclonal antibodies to bovine herpesvirus 1 glycoproteins gB, gC, and gD SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Heterohybridoma; Bovine monoclonal antibody; Bovine herpesvirus 1; Virus glycoprotein; Virus neutralization; Passive immunity ID HERPES-SIMPLEX-VIRUS; RESPIRATORY SYNCYTIAL VIRUS; RHINOTRACHEITIS VIRUS; CELL-LINE; MOUSE HETEROHYBRIDOMA; TYPE-1 GLYCOPROTEINS; INTERSPECIES FUSION; HUMAN-CHROMOSOMES; ANTIGENIC SITES; INFECTION AB Seventy-eight heterohybridomas (HH) stably secreting bovine monoclonal antibodies (BomAb) to Bovine herpesvirus 1 (BHV1) were produced by fusing lymph node cells from a BHV1 hyperimmunized calf with 3 types of non-secreting fusion partners. Seven were generated through fusion with the murine x murine (murine(2)) hybridoma 5P2/0,3 through fusion with bovine-murine(2) HH previously generated using cells from the same calf, and 68 through fusion with bovine(2)-murine(2) HH previously generated by sequential fusions using cells from the same calf. The chromosome number of example HH increased with increasing numbers of input fusions. A variety of indirect fluorescent antibody assay patterns was observed using the BomAb, suggesting diverse antigen specificity. Three bovine(3)-murine(2) HR secreted IgG1 BomAb neutralizing BHV1 without complement, and were chosen for further characterization. SDS-PAGE of detergent-solubilized BHV1 proteins bound to the 3 neutralizing BomAb demonstrated their individual specificities for BHV1 envelope glycoproteins gB, gC, and gD, the major neutralization targets for BHV1. The 3 HH stably secreted the BomAb in culture for over one year, and pilot-scale production of the BomAb was accomplished by in vivo and in vitro methods. A cocktail of the 3 BomAb was administered intravenously (i.v.) to a 6-month-old calf and its serum neutralization activity decreased with a half-life consistent with non-immune clearance, suggesting that BomAb may be useful for passive immune treatment of disease in cattle. Rabbits were passively protected by i.v. injection with each of the anti-gB and anti-gD BomAb when challenged i.v. with BHV1 24 h later. Self re-fusion was shown to be advantageous for efficiently producing HH stably secreting host monoclonal antibodies. The BomAb described should prove useful in studies of the host immune response to BHV1, as reagents, and as sources of bovine immunoglobulin sequences. Published by Elsevier B.V. C1 [Levings, Randall L.] APHIS, Sci Technol & Anal Serv, VS, USDA, Ames, IA 50010 USA. [Stoll, Ione R.; Patterson, Peggy A.] APHIS, Ctr Vet Biol, VS, USDA, Ames, IA 50010 USA. [Warg, Janet V.] APHIS, Natl Vet Serv Labs, VS, USDA, Ames, IA 50010 USA. [Hobbs, Lea Ann] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Kaeberle, Merlin L.; Roth, James A.] Iowa State Univ, Coll Vet Med, Ames, IA 50011 USA. RP Levings, RL (reprint author), APHIS, Sci Technol & Anal Serv, VS, USDA, Ames, IA 50010 USA. EM Randall.L.Levings@aphis.usda.gov RI Roth, James/A-7122-2009 OI Roth, James/0000-0003-3562-668X FU CVB; VS; APHIS; USDA FX Thanks also to Drs. Jose R. Diez, Donna M. Gatewood, Geetha B. Srinivas and Alethea M. Fry of VS, APHIS, USDA and Drs. Kenneth B. Platt, Brett A. Sponseller, John E. Mayfield and Jin-Kyoung Yoon of Iowa State University for critical review of the manuscript. Financial support was provided by the CVB, VS, APHIS, USDA. NR 101 TC 2 Z9 2 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD MAY 15 PY 2014 VL 159 IS 1-2 BP 58 EP 73 DI 10.1016/j.vetimm.2014.02.004 PG 16 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA AH7YE UT WOS:000336350500007 PM 24629764 ER PT J AU Dinh, H Hong, YH Lillehoj, HS AF Dinh, Hue Hong, Yeong Ho Lillehoj, Hyun S. TI Modulation of microRNAs in two genetically disparate chicken lines showing different necrotic enteritis disease susceptibility SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Chicken; Transcriptome; miRNA; Necrotic enteritis; Eimeria maxima; Clostridium perfringens ID CLOSTRIDIUM-PERFRINGENS; CELL DIFFERENTIATION; BROILER-CHICKENS; EIMERIA-MAXIMA; RISK-FACTORS; T-CELLS; EXPRESSION; RESPONSES; NEUTROPHILS; GENERATION AB MicroRNAs (miRNA) play a critical role in post-transcriptional regulation by influencing the 3'-UTR of target genes. Using two inbred White Leghorn chicken lines, line 6.3 and line 7.2 showing Marek's disease-resistant and -susceptible phenotypes, respectively, we used small RNA high-throughput sequencing (HTS) to investigate whether miRNAs are differently expressed in these two chicken lines after inducing necrotic enteritis (NE). The 12 miRNAs, selected from the most down-regulated or up-regulated miRNAs following NE induction, were confirmed by their expressions in real-time PCR. Among these miRNAs, miR-215, miR-217, miR-194, miR-200a, miR-200b, miR-216a, miR-216b, and miR-429 were highly expressed in intestine derived from line 7.2, whereas, miR-1782 and miR-499 were down-regulated. In spleen, miR-34b and miR-1684 were the most up-regulated miRNAs in line 6.3. Notably, five out of six target genes, CXCR5, BCL2, GJA1, TCF12, and TAB3 were differentially expressed between line 6.3 and line 7.2, and showed suppression in the MD-susceptible chicken line. Their expression levels were conversely correlated with those of miRNA obtained from both HTS and quantitative real-time PCR. These results suggest that some miRNAs are differentially altered in response to NE and they modulate the expression of their target genes in the two inbred lines. Collectively, HTS analysis of intestinal miRNAs from NE-afflicted inbred chickens showing different disease phenotypes led to the identification of host immunity genes regulated by miRNA. Future studies of the function of these miRNAs and their target genes in the host will lead to enhanced understanding of molecular mechanisms controlling host-pathogen interaction in NE. (C) 2014 Elsevier B.V. All rights reserved. C1 [Dinh, Hue; Hong, Yeong Ho] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [Lillehoj, Hyun S.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. RP Hong, YH (reprint author), Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. EM yhong@cau.ac.kr FU Rural Development Administration, Republic of Korea [PJ008084] FX The project was supported by the Next-Generation BioGreen 21 Program (no. PJ008084), Rural Development Administration, Republic of Korea. The inbred chicken lines were obtained from ADOL (Avian Disease and Oncology Laboratory), USDA-Agricultural Research Service, East Lansing, MI. NR 46 TC 3 Z9 4 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD MAY 15 PY 2014 VL 159 IS 1-2 BP 74 EP 82 DI 10.1016/j.vetimm.2014.02.003 PG 9 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA AH7YE UT WOS:000336350500008 PM 24629767 ER PT J AU Lee, SH Lillehoj, HS Jeong, M Del Cacho, E Min, W Sullivan, YB Kakach, L LaBresh, JW Kim, HR AF Lee, Sung Hyen Lillehoj, Hyun S. Jeong, Misun Del Cacho, Emilio Min, Wongi Sullivan, Yvonne B. Kakach, Laura LaBresh, Joanna W. Kim, Haeng Ran TI Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Chickens; TL1A; TNFSF15; Hybridoma; Lymphocyte; Macrophage ID NECROTIC ENTERITIS; T-CELL; FUNCTIONAL-CHARACTERIZATION; MOLECULAR-CLONING; BROILER-CHICKENS; NK-LYSIN; EXPRESSION; GAMMA; DR3; COSTIMULATOR AB Tumor necrosis factor-like ligand 1A (TL1A) is a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. However, there have not been any mAbs which specifically detect chTL1A and define its biochemical and immunological properties. So in this study, two mouse monoclonal antibodies (mAbs) which specifically detect chicken TL1A (chTL1A) were developed and characterized. Both mAbs identified a 32 kDa Escherichia coli-derived, poly-histidine-tagged fusion protein by Western blot analysis. The mAbs identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry, and were used to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens by antigen capture ELISA. These mAbs inhibited chTL1A-induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9). These new mAbs that detect chTL1A will be important immune reagents for basic and applied research in poultry immunology. Published by Elsevier B.V. C1 [Lee, Sung Hyen; Lillehoj, Hyun S.; Jeong, Misun] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Del Cacho, Emilio] Univ Zaragoza, Dept Anim Pathol, Fac Vet Sci, Zaragoza 500015, Spain. [Min, Wongi] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea. [Min, Wongi] Gyeongsang Natl Univ, Life Sci Res Inst, Jinju 660701, Gyeongnam, South Korea. [Sullivan, Yvonne B.; Kakach, Laura; LaBresh, Joanna W.] Kingfisher Biotech Inc, St Paul, MN 55114 USA. [Lee, Sung Hyen; Kim, Haeng Ran] Rural Dev Adm, Natl Acad Agr Sci, Suwon 441853, Gyeonggi, South Korea. RP Lillehoj, HS (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov OI Min, Wongi/0000-0003-2437-7366 FU National Research Initiative of the USDA; U.S. Veterinary Immune Reagent Network Grant [2010-65121-20649]; U.S. Veterinary Immune Reagent Network Grant (USDA-CSREES) [2005-01812]; WCU program through the National Research Foundation of Korea - Ministry of Education, Science and Technology [R33-10013]; ARS-NVRQS trust [1245-32000-097-07]; Rural Development Administration, Republic of Korea [PJ009386] FX This project was supported, in part, by the National Research Initiative of the USDA, U.S. Veterinary Immune Reagent Network Grant (NIFA no. 2010-65121-20649, USDA-CSREES no. 2005-01812), the WCU program through the National Research Foundation of Korea sponsored by the Ministry of Education, Science and Technology (R33-10013), and ARS-NVRQS trust agreement (1245-32000-097-07), and the project 'Anti-asthma effect of Salvia plebeia R. Br. and Adenophora triphylla var. japonica (PJ009386), of Rural Development Administration, Republic of Korea. The authors thank Dr. Seung I Jang, Dr. Duk Kyung Kim, Ms. Marjorie Nichols, Ms. Stacy O'Donnell, Ms. Myeongseon Park, and Mr. Seung Kyu Lee for their contributions to this research. NR 26 TC 1 Z9 1 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD MAY 15 PY 2014 VL 159 IS 1-2 BP 103 EP 109 DI 10.1016/j.vetimm.2014.01.002 PG 7 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA AH7YE UT WOS:000336350500012 PM 24565040 ER PT J AU Emerich, DW Krishnan, HB AF Emerich, David W. Krishnan, Hari B. TI Symbiosomes: temporary moonlighting organelles SO BIOCHEMICAL JOURNAL LA English DT Review DE legume; moonlighting protein; nitrogen fixation; nodule; rhizobia; symbiosome ID SOYBEAN ROOT-NODULES; BRADYRHIZOBIUM-JAPONICUM BACTEROIDS; RHIZOBIUM-LEGUME SYMBIOSIS; NITROGEN-FIXATION; MEDICAGO-TRUNCATULA; PERIBACTEROID SPACE; GLUTAMINE-SYNTHETASE; GLYCINE-MAX; MOLECULAR CHAPERONES; BACTERIAL VIRULENCE AB Symbiosomes are a unique structural entity that performs the role of biological nitrogen fixation, an energy-demanding process that is the primary entryway of fixed, nitrogen into the biosphere. Symbiosomes result from the infection of specific rhizobial strains into the roots of an appropriate leguminous host plant forming an organ referred to as a nodule. Within the infected plant cells of the nodule, the rhizobia are encased within membrane-bounded structures that develop into symbiosomes. Mature symbiosomes create an environment that allows the rhizobia to differentiate into a nitrogen-fixing form called bacteroids. The bacteroids are surrounded by the symbiosome space, which is populated by proteins from both eukaryotic and prokaryotic symbionts, suggesting this space is the quintessential component of symbiosis: an inter-kingdom environment with the single purpose of symbiotic nitrogen fixation. Proteins associated with the symbiosome membrane are largely plant-derived proteins and are non-metabolic in nature. The proteins of the symbiosome space are mostly derived from the bacteroid with annotated functions of carbon metabolism, whereas relatively few are involved in nitrogen metabolism. An appreciable portion of both the eukaryotic and prokaryotic proteins in the symbiosome are also 'moonlighting' proteins, which are defined as proteins that perform roles unrelated to their annotated activities when found in an unexpected physiological environment. The essential functions of symbiotic nitrogen fixation of the symbiosome are performed by co-operative interactions of proteins from both symbionts some of which may be performing unexpected roles. C1 [Emerich, David W.] Univ Missouri, Div Biochem, Columbia, MO 65211 USA. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Emerich, DW (reprint author), Univ Missouri, Div Biochem, 117 Schweitzer Hall, Columbia, MO 65211 USA. EM EmerichD@Missouri.edu NR 124 TC 5 Z9 5 U1 1 U2 38 PU PORTLAND PRESS LTD PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND SN 0264-6021 EI 1470-8728 J9 BIOCHEM J JI Biochem. J. PD MAY 15 PY 2014 VL 460 BP 1 EP 11 DI 10.1042/BJ20130271 PN 1 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AH4CU UT WOS:000336075200001 PM 24762136 ER PT J AU Behravesh, CB Brinson, D Hopkins, BA Gomez, TM AF Behravesh, Casey Barton Brinson, Denise Hopkins, Brett A. Gomez, Thomas M. TI Backyard Poultry Flocks and Salmonellosis: A Recurring, Yet Preventable Public Health Challenge SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE Salmonella; zoonoses; outbreak; backyard poultry; mail-order hatchery ID MAIL-ORDER HATCHERY; UNITED-STATES; MULTISTATE OUTBREAK; LIVE POULTRY; CONTACT; SURVEILLANCE; TYPHIMURIUM; MANAGEMENT; PATHOGENS; CHILDREN AB Poultry are well recognized as possible carriers of Salmonella species. As part of the local foods movement, backyard poultry flocks have increased in popularity in recent years. Between 1996 and 2012, 45 outbreaks of human Salmonella infections linked to live poultry from mail-order hatcheries were documented. This review examines the history of live poultry-associated salmonellosis in humans in the United States, the current status of the issue, and what can be done to help prevent these illnesses. An integrated One Health approach involving the mail-order hatchery industry, feed stores, healthcare providers, veterinarians, and backyard flock owners is needed to help prevent live poultry-associated salmonellosis. C1 [Behravesh, Casey Barton] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging Zoonot & Infect Dis, Atlanta, GA 30329 USA. [Brinson, Denise] USDA, Natl Poultry Improvement Plan, Conyers, GA USA. [Hopkins, Brett A.] Int Tech Anim Prod & Proc Solut, Overland Pk, KS USA. [Gomez, Thomas M.] USDA, Vet Serv, Anim & Plant Hlth Inspect Serv, Atlanta, GA USA. RP Behravesh, CB (reprint author), Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, 1600 Clifton Rd NE,MS A38, Atlanta, GA 30329 USA. EM cbartonbehravesh@cdc.gov FU Centers for Disease Control and Prevention FX This work was supported by the Centers for Disease Control and Prevention. NR 34 TC 17 Z9 18 U1 2 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD MAY 15 PY 2014 VL 58 IS 10 BP 1432 EP 1438 DI 10.1093/cid/ciu067 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA AH3RZ UT WOS:000336044200018 PM 24501387 ER PT J AU Joy, SR Li, X Snow, DD Gilley, JE Woodbury, B Bartelt-Hunt, SL AF Joy, Stacey R. Li, Xu Snow, Daniel D. Gilley, John E. Woodbury, Bryan Bartelt-Hunt, Shannon L. TI Fate of antimicrobials and antimicrobial resistance genes in simulated swine manure storage SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Antimicrobial; Antimicrobial resistance genes; Bacitracin; Chlortetracycline; Swine manure; Tylosin ID TANDEM MASS-SPECTROMETRY; ANTIBIOTIC-RESISTANCE; TETRACYCLINE RESISTANCE; LAND APPLICATION; VETERINARY ANTIBIOTICS; BACTERIAL-RESISTANCE; AMENDED SOIL; WASTE; DEGRADATION; ENVIRONMENT AB The behavior of three antibiotics (bacitracin, chlortetracycline, and tylosin) and two classes of antibiotic resistance genes (ARGs), tet and erm, were monitored in swine manure slurry under anaerobic conditions. First-order decay rates were determined for each antibiotic with half-lives ranging from I day (chlortetracycline) to 10 days (tylosin). ARGs were monitored in the swine manure slurry, and losses of approximately 1 to 3 orders of magnitude in relative abundance were observed during the 40 day storage period. First-order degradation profiles were observed for chlortetracycline and its corresponding resistance genes, tet(X) and tet(Q). Tylosin was degraded to approximately 10% of the starting concentration by day 40; however, the relative abundance of erm(B) remained at 50-60% of the initial relative abundance while the relative abundance of erm(F) decreased by 80-90%, consistent with tylosin. These results indicate that tet resistance genes respond primarily to chlortetracycline antimicrobials, and may be lost when the parent tetracycline compound is degraded. In contrast, erm(B) resistance gene may respond to a range of antimicrobials in animal manure, and may persist despite losses of tylosin. (C) 2014 Elsevier B.V. All rights reserved. C1 [Joy, Stacey R.; Li, Xu; Bartelt-Hunt, Shannon L.] Univ Nebraska, Dept Civil Engn, Lincoln, NE 68588 USA. [Snow, Daniel D.] Univ Nebraska, Sch Nat Resources, Water Sci Lab, Lincoln, NE 68503 USA. [Gilley, John E.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Woodbury, Bryan] USDA Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Bartelt-Hunt, SL (reprint author), Univ Nebraska, Dept Civil Engn, Lincoln, NE 68588 USA. EM sbartelt2@unl.edu RI Li, Xu/K-1957-2014 FU National Pork Checkoff FX Funding, wholly or in part, was provided by the National Pork Checkoff. NR 48 TC 14 Z9 17 U1 9 U2 85 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 15 PY 2014 VL 481 BP 69 EP 74 DI 10.1016/j.scitotenv.2014.02.027 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA AG0HL UT WOS:000335096400009 PM 24583946 ER PT J AU Bullied, WJ Flerchinger, GN Bullock, PR Van Acker, RC AF Bullied, W. John Flerchinger, Gerald N. Bullock, Paul R. Van Acker, Rene C. TI Process-based modeling of temperature and water profiles in the seedling recruitment zone: Part I. Model validation SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Microclimate; Process-based modeling; Seedling recruitment zone; SHAW model; Soil temperature; Soil water ID SURFACE SOIL-TEMPERATURE; SPRING WHEAT; HYDROTHERMAL TIME; SIMULTANEOUS HEAT; CANOPY MODEL; WINTER-WHEAT; GROWTH; RADIATION; MOISTURE; RESIDUE AB Process-based modeling provides greater spatial and temporal information of the soil environment in the shallow seedling recruitment zone across field topography where measurements of soil temperature and water may not sufficiently describe the zone. Hourly temperature and water profiles within the 75 mm recruitment zone for 75 days after seeding were simulated for Canadian Prairie conditions from the process-based Simultaneous Heat and Water (SHAW) model using local and non-local microclimatic data. Measured and modeled soil cover and spring wheat vegetative cover were used to parameterize the model. Heat and water transfer was simulated through surface residue, early vegetation and soil. Simulations were evaluated using model efficiency, root mean square deviation, and components of mean squared error. The greatest amount of error in simulated soil temperature was lack of correlation in the fluctuation pattern over time, followed by bias of the simulation. Soil temperature simulations had model efficiency of 0.87, overestimation of 0.4 degrees C, and a RMSD of 2.1 degrees C averaged across all topographical factors and soil depths. Simulations of soil water had low model efficiency and RMSD of 0.55 MPa. Average absolute bias for soil water was 0.27 MPa which reflected predominantly positive bias at the soil surface and 0-25 mm soil layer and negative bias in the 25-50 and 50-75 mm soil layers. Process-based modeling using microclimatic information was shown to provide representative simulations of the soil environment for all depths of the seedling recruitment zone. (C) 2013 Elsevier B.V. All rights reserved. C1 [Bullied, W. John; Van Acker, Rene C.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Bullock, Paul R.] Univ Manitoba, Dept Soil Sci, Winnipeg, MB R3T 2N2, Canada. RP Bullied, WJ (reprint author), Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. EM john.bullied@uoguelph.ca; gerald.flerchinger@ars.usda.gov; paul.bullock@umanitoba.ca; vanacker@uoguelph.ca RI Bullock, Paul/B-3948-2010 FU Manitoba Rural Adaptation Council; Natural Sciences and Engineering Research Council of Canada; Canadian Wheat Board; University of Guelph FX This research was funded by the Manitoba Rural Adaptation Council, the Natural Sciences and Engineering Research Council of Canada, the Canadian Wheat Board, and the University of Guelph. NR 64 TC 2 Z9 3 U1 0 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 15 PY 2014 VL 188 BP 89 EP 103 DI 10.1016/j.agrformet.2013.11.012 PG 15 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AB5WX UT WOS:000331860500009 ER PT J AU Bullied, WJ Bullock, PR Flerchinger, GN Van Acker, RC AF Bullied, W. John Bullock, Paul R. Flerchinger, Gerald N. Van Acker, Rene C. TI Process-based modeling of temperature and water profiles in the seedling recruitment zone: Part II. Seedling emergence timing SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Hydrothermal time; Seedling emergence timing; Seedling recruitment zone; Soil temperature; Soil water; Spring wheat ID GERMINATION RESPONSE; HYDROTHERMAL TIME; SOIL-TEMPERATURE; WHEAT; SURFACE; DEPTH; PREDICTION; MOISTURE; TILLAGE; GROWTH AB Predictions of seedling emergence timing for spring wheat are facilitated by process-based modeling of the microsite environment in the shallow seedling recruitment zone. Hourly temperature and water profiles within the recruitment zone for 75 days after planting were simulated from the process-based Simultaneous Heat and Water (SHAW) model using local and non-local microclimatic data. Linear mixed-effects models indicated that simulated thermal and hydrothermal time accumulations were similar to measurements. Emergence timing was fitted using the Gompertz equation. Simulations averaged across depth had quicker emergence timing of wheat at inflection by 20 degrees Cd for thermal time and 23 MPa degrees Cd for hydrothermal time models, equating to 1.3 days earlier in the DAP model. Seedling emergence rates were similar between simulations and measurements. Simulations for emergence timing with hydrothermal time improved upon thermal time only at the soil surface. The recruitment depth of spring wheat over time was fitted with a Beta function which was positively skewed with early recruitment of a large number of seedlings from a moderate depth and late recruitment by a small number of seedlings from a shallow depth. The time of simulated Beta maxima was greater by 39 degrees Cd for thermal time and 3 MPa degrees Cd for hydrothermal time, and 1.5 days less than the measured maxima for the DAP model. The 95% confidence intervals for the fitted simulation and measured Beta functions overlapped for the entire duration of the distribution for all time scale models. Process-based simulations of soil temperature and soil water in the seedling recruitment zone provided representative predictions of seedling emergence timing for spring wheat. (C) 2013 Elsevier B.V. All rights reserved. C1 [Bullied, W. John; Van Acker, Rene C.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Bullock, Paul R.] Univ Manitoba, Dept Soil Sci, Winnipeg, MB R3T 2N2, Canada. [Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Bullied, WJ (reprint author), Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. EM john.bullied@uoguelph.ca; paul.bullock@umanitoba.ca; gerald.flerchinger@ars.usda.gov; vanacker@uoguelph.ca RI Bullock, Paul/B-3948-2010 FU Manitoba Rural Adaptation Council; Natural Sciences and Engineering Research Council of Canada; Canadian Wheat Board; University of Guelph FX This research was funded by the Manitoba Rural Adaptation Council, the Natural Sciences and Engineering Research Council of Canada, the Canadian Wheat Board, and the University of Guelph. NR 38 TC 1 Z9 1 U1 1 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 15 PY 2014 VL 188 BP 104 EP 120 DI 10.1016/j.agrformet.2013.10.007 PG 17 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AB5WX UT WOS:000331860500010 ER PT J AU Xiao, ZL Lester, GE Luo, YG Xie, ZH Yu, LL Wang, Q AF Xiao, Zhenlei Lester, Gene E. Luo, Yaguang Xie, Zhuohong (Kenny) Yu, Liangli (Lucy) Wang, Qin TI Effect of light exposure on sensorial quality, concentrations of bioactive compounds and antioxidant capacity of radish microgreens during low temperature storage SO FOOD CHEMISTRY LA English DT Article DE Radish microgreens; Light exposure; Quality maintenance; Ascorbic acid; Total phenolics; Antioxidant capacity ID CUT ROMAINE LETTUCE; VIOLAXANTHIN CYCLE; ASCORBIC-ACID; BROCCOLI; VITAMIN; INHIBITION; MECHANISM; PHENOLICS; ASSAY AB Radish microgreens constitute a good source of bioactive compounds; however, they are very delicate and have a short shelf life. In this study, we investigated the impact of light exposure and modified atmosphere packaging on sensorial quality, bioactive compound concentrations and antioxidant capacity of radish microgreens during storage. Results showed that light exposure during storage increased the amount of ascorbic acid and had no effect on alpha-tocopherol or total phenolic concentrations. Dark storage resulted in higher hydroxyl radical scavenging capacity and carotenoid retention. No significant differences were found for relative 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity between light and dark treatments. Radish microgreens in bags of 29.5 pmol s(-1) m(-2) Pa-1 oxygen transmission rate (OTR) maintained better quality than those within laser microperforated bags. In conclusion, light exposure accelerated deterioration of radish microgreens, while dark storage maintained quality; and application of OTR bags was beneficial in extending shelf life. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Xiao, Zhenlei; Xie, Zhuohong (Kenny); Yu, Liangli (Lucy); Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Lester, Gene E.; Luo, Yaguang] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Wang, Q (reprint author), Univ Maryland, Dept Nutr & Food Sci, 0112 Skinner Bldg, College Pk, MD 20742 USA. EM wangqin@umd.edu RI Xie, Zhuohong/P-3599-2014 OI Xie, Zhuohong/0000-0001-8009-3523 FU USDA-NIFA Specialty Crop Research Initiative Grant [MDW-2010-01165] FX This work was supported by USDA-NIFA Specialty Crop Research Initiative Grant Award No. MDW-2010-01165. The authors thank Ms. Ellen Turner for helping us with the statistical analysis. NR 30 TC 9 Z9 10 U1 5 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAY 15 PY 2014 VL 151 BP 472 EP 479 DI 10.1016/j.foodchem.2013.11.086 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AB2AP UT WOS:000331595700069 PM 24423559 ER PT J AU Haack, RA Britton, KO Brockerhoff, EG Cavey, JF Garrett, LJ Kimberley, M Lowenstein, F Nuding, A Olson, LJ Turner, J Vasilaky, KN AF Haack, Robert A. Britton, Kerry O. Brockerhoff, Eckehard G. Cavey, Joseph F. Garrett, Lynn J. Kimberley, Mark Lowenstein, Frank Nuding, Amelia Olson, Lars J. Turner, James Vasilaky, Kathryn N. TI Effectiveness of the International Phytosanitary Standard ISPM No. 15 on Reducing Wood Borer Infestation Rates in Wood Packaging Material Entering the United States SO PLOS ONE LA English DT Article ID EMERALD ASH BORER; HEAT-TREATMENT; EXOTIC BARK; SPECIES INTRODUCTIONS; AMBROSIA BEETLES; COLEOPTERA; BUPRESTIDAE; INVASIONS; US; INTERCEPTIONS AB Numerous bark- and wood-infesting insects have been introduced to new countries by international trade where some have caused severe environmental and economic damage. Wood packaging material (WPM), such as pallets, is one of the high risk pathways for the introduction of wood pests. International recognition of this risk resulted in adoption of International Standards for Phytosanitary Measures No. 15 (ISPM15) in 2002, which provides treatment standards for WPM used in international trade. ISPM15 was originally developed by members of the International Plant Protection Convention to "practically eliminate" the risk of international transport of most bark and wood pests via WPM. The United States (US) implemented ISPM15 in three phases during 2005-2006. We compared pest interception rates of WPM inspected at US ports before and after US implementation of ISPM15 using the US Department of Agriculture AQIM (Agriculture Quarantine Inspection Monitoring) database. Analyses of records from 2003-2009 indicated that WPM infestation rates declined 36-52% following ISPM15 implementation, with results varying in statistical significance depending on the selected starting parameters. Power analyses of the AQIM data indicated there was at least a 95% chance of detecting a statistically significant reduction in infestation rates if they dropped by 90% post-ISPM15, but the probability fell as the impact of ISPM15 lessened. We discuss several factors that could have reduced the apparent impact of ISPM15 on lowering WPM infestation levels, and suggest ways that ISPM15 could be improved. The paucity of international interception data impeded our ability to conduct more thorough analyses of the impact of ISPM15, and demonstrates the need for well-planned sampling programs before and after implementation of major phytosanitary policies so that their effectiveness can be assessed. We also present summary data for bark- and wood-boring insects intercepted on WPM at US ports during 1984-2008. C1 [Haack, Robert A.] US Forest Serv, USDA, No Res Stn, Lansing, MI USA. [Britton, Kerry O.] US Forest Serv, USDA, Arlington, VA USA. [Brockerhoff, Eckehard G.] Scion NZ Forest Res Inst, Christchurch, New Zealand. [Cavey, Joseph F.] US Anim & Plant Hlth Inspect Serv, USDA, Plant Hlth Programs, Natl Identificat Serv, Riverdale, MD USA. [Garrett, Lynn J.] US Anim & Plant Hlth Inspect Serv, USDA, Ctr Plant Hlth Sci & Technol, Raleigh, NC USA. [Kimberley, Mark] Scion New Zealand Forest Res Inst, Rotorua, New Zealand. [Lowenstein, Frank] New England Forestry Fdn, Littleton, MA USA. [Nuding, Amelia] Univ Calif Santa Barbara, Natl Ctr Ecol Anal & Synth, Santa Barbara, CA 93106 USA. [Olson, Lars J.] Univ Maryland, College Pk, MD 20742 USA. [Turner, James] AgResearch Ltd, Ruakura Res Ctr, Hamilton, New Zealand. [Vasilaky, Kathryn N.] Columbia Univ, Earth Inst, New York, NY USA. [Vasilaky, Kathryn N.] Columbia Univ, Int Res Inst Climate & Soc, New York, NY USA. RP Haack, RA (reprint author), US Forest Serv, USDA, No Res Stn, Lansing, MI USA. EM rhaack@fs.fed.us RI Brockerhoff, Eckehard/C-1528-2009 OI Brockerhoff, Eckehard/0000-0002-5962-3208 FU Nature Conservancy; National Science Foundation [EF-0553768]; University of California Santa Barbara; State of California; New Zealand Foundation for Research and Technology [C02X0501, C04X0302] FX The Working Group "Effects of trade policy on management of non-native forest pests and pathogens" was supported by a grant from The Nature Conservancy to the National Center for Ecological Analysis and Synthesis, which is a Center funded by the National Science Foundation (Grant #EF-0553768), the University of California Santa Barbara, and the State of California. Partial funding was provided by the New Zealand Foundation for Research and Technology through contracts C02X0501 (Better Border Biosecurity) and C04X0302 (Forest Biosecurity and Protection) to the author EGB. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 25 Z9 27 U1 1 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 14 PY 2014 VL 9 IS 5 AR e96611 DI 10.1371/journal.pone.0096611 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI4TJ UT WOS:000336857400031 PM 24827724 ER PT J AU Moghaddam, SM Song, QJ Mamidi, S Schmutz, J Lee, R Cregan, P Osorno, JM McClean, PE AF Moghaddam, Samira Mafi Song, Qijian Mamidi, Sujan Schmutz, Jeremy Lee, Rian Cregan, Perry Osorno, Juan M. McClean, Phillip E. TI Developing market class specific In Del markers from next generation sequence data in Phaseolus vulgaris L. SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE InDel marker; next generation sequencing; market class; phylogenetics; genetic map; common bean ID MAP-BASED CLONING; COMMON BEAN LANDRACES; GENETIC DIVERSITY; INDEL MARKERS; LINKAGE MAP; MICROSATELLITE MARKERS; MULTIPLEX PCR; DNA SAMPLES; WILD; DOMESTICATION AB Next generation sequence data provides valuable information and tools for genetic and genomic research and offers new insights useful for marker development. This data is useful for the design of accurate and user-friendly molecular tools. Common bean (Phaseolus vulgaris L.) is a diverse crop in which separate domestication events happened in each gene pool followed by race and market class diversification that has resulted in different morphological characteristics in each commercial market class. This has led to essentially independent breeding programs within each market class which in turn has resulted in limited within market class sequence variation. Sequence data from selected genotypes of five bean market classes (pinto, black, navy, and light and dark red kidney) were used to develop InDel-based markers specific to each market class. Design of the InDel markers was conducted through a combination of assembly, alignment and primer design software using 1.6x to 5.1x coverage of Illumina GAII sequence data for each of the selected genotypes. The procedure we developed for primer design is fast, accurate, less error prone, and higher throughput than when they are designed manually. All InDel markers are easy to run and score with no need for PCR optimization. A total of 2687 InDel markers distributed across the genome were developed. To highlight their usefulness, they were employed to construct a phylogenetic tree and a genetic map, showing that InDel markers are reliable, simple, and accurate. C1 [Moghaddam, Samira Mafi; Mamidi, Sujan; McClean, Phillip E.] N Dakota State Univ, Genom & Bioinformat Program, Fargo, ND 58102 USA. [Moghaddam, Samira Mafi; Mamidi, Sujan; Lee, Rian; Osorno, Juan M.; McClean, Phillip E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58102 USA. [Song, Qijian; Cregan, Perry] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD USA. [Schmutz, Jeremy] HudsonAlpha Inst, Huntsville, AL USA. RP McClean, PE (reprint author), N Dakota State Univ, Genom & Bioinformat Program, 166 Loftsgard Hall,N Bolley Dr, Fargo, ND 58102 USA. EM phillip.mcclean@ndsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU Agriculture and Food Research Initiative Competitive Grants from the USDA National Institute of Food and Agriculture [2009-01929, 2010-03612] FX This project was supported by Agriculture and Food Research Initiative Competitive Grants no. 2009-01929 and 2010-03612 from the USDA National Institute of Food and Agriculture. NR 76 TC 9 Z9 10 U1 2 U2 17 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 13 PY 2014 VL 5 AR 185 DI 10.3389/fpls.2014.00185 PG 13 WC Plant Sciences SC Plant Sciences GA AI5MP UT WOS:000336911800001 PM 24860578 ER PT J AU Fortunato, MJ Ball, CE Hollinger, K Patel, NB Modi, JN Rajasekaran, V Nonneman, DJ Ross, JW Kennedy, EJ Selsby, JT Beedle, AM AF Fortunato, Marisa J. Ball, Charlotte E. Hollinger, Katrin Patel, Niraj B. Modi, Jill N. Rajasekaran, Vedika Nonneman, Dan J. Ross, Jason W. Kennedy, Eileen J. Selsby, Joshua T. Beedle, Aaron M. TI Development of Rabbit Monoclonal Antibodies for Detection of Alpha-Dystroglycan in Normal and Dystrophic Tissue SO PLOS ONE LA English DT Article ID EYE-BRAIN DISEASE; MUSCULAR-DYSTROPHY; GLYCOPROTEIN COMPLEX; SKELETAL-MUSCLE; LAMININ; GLYCOSYLATION; DISRUPTION; DEFICIENCY; EXPRESSION; MEMBRANE AB Alpha-dystroglycan requires a rare O-mannose glycan modification to form its binding epitope for extracellular matrix proteins such as laminin. This functional glycan is disrupted in a cohort of muscular dystrophies, the secondary dystroglycanopathies, and is abnormal in some metastatic cancers. The most commonly used reagent for detection of alpha-dystroglycan is mouse monoclonal antibody IIH6, but it requires the functional O-mannose structure for recognition. Therefore, the ability to detect alpha-dystroglycan protein in disease states where it lacks the full O-mannose glycan has been limited. To overcome this hurdle, rabbit monoclonal antibodies against the alpha-dystroglycan C-terminus were generated. The new antibodies, named 5-2, 29-5, and 45-3, detect alpha-dystroglycan from mouse, rat and pig skeletal muscle by Western blot and immunofluorescence. In a mouse model of fukutin-deficient dystroglycanopathy, all antibodies detected low molecular weight alpha-dystroglycan in disease samples demonstrating a loss of functional glycosylation. Alternately, in a porcine model of Becker muscular dystrophy, relative abundance of alpha-dystroglycan was decreased, consistent with a reduction in expression of the dystrophin-glycoprotein complex in affected muscle. Therefore, these new rabbit monoclonal antibodies are suitable reagents for alpha-dystroglycan core protein detection and will enhance dystroglycan-related studies. C1 [Fortunato, Marisa J.; Kennedy, Eileen J.; Beedle, Aaron M.] Univ Georgia, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. [Ball, Charlotte E.; Patel, Niraj B.; Modi, Jill N.; Rajasekaran, Vedika] Univ Georgia, Ctr Undergrad Res, Athens, GA 30602 USA. [Hollinger, Katrin; Ross, Jason W.; Selsby, Joshua T.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Nonneman, Dan J.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Beedle, AM (reprint author), Univ Georgia, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. EM beedlea@uga.edu RI Beedle, Aaron/K-1902-2015; OI Beedle, Aaron/0000-0002-1019-3235; Selsby, Joshua/0000-0003-3797-7539 FU University of Georgia College of Pharmacy; National Institutes of Health [R21NS079603, R21RR030232] FX This work was supported by the University of Georgia College of Pharmacy (AMB) and by funds from the National Institutes of Health R21NS079603 (JTS) and R21RR030232 (JWR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 31 TC 6 Z9 6 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 13 PY 2014 VL 9 IS 5 AR e97567 DI 10.1371/journal.pone.0097567 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AH8EY UT WOS:000336369200129 PM 24824861 ER PT J AU Fitzgerald, TD Wolfin, M Rossi, F Carpenter, JE Pescador-Rubio, A AF Fitzgerald, Terrence D. Wolfin, Michael Rossi, Frank Carpenter, James E. Pescador-Rubio, Alfonso TI Trail marking by larvae of the cactus moth, Cactoblastis cactorum SO JOURNAL OF INSECT SCIENCE LA English DT Article DE mandibular glands; social caterpillar; silk; trail pheromone; cactus caterpillar; 2-acyl-1,3 cyclohexane dione ID MANDIBULAR GLAND SECRETION; EASTERN TENT CATERPILLARS; INDIAN-MEAL MOTH; PLODIA-INTERPUNCTELLA; EPHESTIA-KUEHNIELLA; YPONOMEUTA-CAGNAGELLUS; ANAGASTA-KUEHNIELLA; EXOCRINE GLANDS; FLOUR MOTH; LEPIDOPTERA AB The cactus moth, Cactoblastis cactorum (Berg) (Lepidoptera: Pyralidae), spends most of its larval life feeding within the cladodes of Opuntia cactuses, but the gregarious caterpillars begin their life outside the plant, and in the later instars make intermittent excursions over plant surfaces to access new cladodes and to thermoregulate. The study reported here showed that when the caterpillars move en masse, they mark and follow trails that serve to keep the cohort together. Artificial trails prepared from hexane extracts of the caterpillar's paired mandibular glands were readily followed by the caterpillars. The glands are remarkably large, and their fluid contents, which constitute approximately 1% of the total wet mass of a caterpillar, are secreted onto the substrate as they move. Although the caterpillars also lay down copious quantities of silk, the material in itself neither elicits trail following nor is it a requisite component of pathways that elicit trail following. Previous analyses of the mandibular glands of other species of pyralid caterpillars showed that they contain a series of structurally distinct 2-acyl-1,3 cyclohexane diones. Chemical analysis indicates that the glands of C. cactorum contain structurally similar compounds, and bioassays indicate that trail following occurs in response to these chemicals. While the mandibular glands' fluids have been shown to act as semiochemicals, effecting both interspecific and intraspecific behavior in other species of pyralids, the present study is the first to report their use as a trail pheromone. C1 [Fitzgerald, Terrence D.; Wolfin, Michael] SUNY Coll Cortland, Dept Biol Sci, Cortland, NY 13045 USA. [Wolfin, Michael; Rossi, Frank] SUNY Coll Cortland, Dept Chem, Cortland, NY 13045 USA. [Carpenter, James E.] USDA ARS, Crop Protect & Res Lab, Tifton, GA 31793 USA. [Pescador-Rubio, Alfonso] Univ Colima, Ctr Univ Invest & Desarrollo Agr, Tecoman 28100, Colima, Mexico. RP Fitzgerald, TD (reprint author), SUNY Coll Cortland, Dept Biol Sci, Cortland, NY 13045 USA. EM Fitzgerald@cortland.edu; Michael.Wolfin@cortland.edu; Frank.Rossi@cortland.edu; Jim.Carpenter@ARS.USDA.GOV; apescado@yahoo.com FU Programa Integral de Fortalecimiento Institucional - Secretaria de Educacion Publica; USDA; APHIS-PPQ FX Colonies were maintained under APHIS permit P526P-09-00995. We thank Susan Drawdy for providing egg masses of C. cactorum and Brandon Milliken for assisting with the chemistry. APR received funds from "Programa Integral de Fortalecimiento Institucional 2009-2010 - Secretaria de Educacion Publica." This research was supported in part by USDA, APHIS-PPQ, with funding through Farm Bill Section 10201. Mention of trade names or commercial products in this article does not imply recommendation or endorsement by APHISPPQ. NR 49 TC 0 Z9 1 U1 0 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD MAY 13 PY 2014 VL 14 AR 64 PG 15 WC Entomology SC Entomology GA AH1MG UT WOS:000335884300001 PM 25373211 ER PT J AU McGuire, KJ Torgersen, CE Likens, GE Buso, DC Lowe, WH Bailey, SW AF McGuire, Kevin J. Torgersen, Christian E. Likens, Gene E. Buso, Donald C. Lowe, Winsor H. Bailey, Scott W. TI Network analysis reveals multiscale controls on streamwater chemistry SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE biogeochemistry; hydrologic connectivity; watershed; autocorrelation; heterogeneity ID TERM ECOLOGICAL RESEARCH; MOVING-AVERAGE APPROACH; HUBBARD-BROOK-VALLEY; LANDSCAPE ECOLOGY; SPATIAL HETEROGENEITY; HEADWATER STREAMS; NITROGEN EXPORT; PATTERN; WATER; TERRESTRIAL AB By coupling synoptic data from a basin-wide assessment of streamwater chemistry with network-based geostatistical analysis, we show that spatial processes differentially affect biogeochemical condition and pattern across a headwater stream network. We analyzed a high-resolution dataset consisting of 664 water samples collected every 100 m throughout 32 tributaries in an entire fifth-order stream network. These samples were analyzed for an exhaustive suite of chemical constituents. The fine grain and broad extent of this study design allowed us to quantify spatial patterns over a range of scales by using empirical semivariograms that explicitly incorporated network topology. Here, we show that spatial structure, as determined by the characteristic shape of the semivariograms, differed both among chemical constituents and by spatial relationship (flow-connected, flow-unconnected, or Euclidean). Spatial structure was apparent at either a single scale or at multiple nested scales, suggesting separate processes operating simultaneously within the stream network and surrounding terrestrial landscape. Expected patterns of spatial dependence for flow-connected relationships (e.g., increasing homogeneity with downstream distance) occurred for some chemical constituents (e.g., dissolved organic carbon, sulfate, and aluminum) but not for others (e.g., nitrate, sodium). By comparing semivariograms for the different chemical constituents and spatial relationships, we were able to separate effects on streamwater chemistry of (i) fine-scale versus broad-scale processes and (ii) in-stream processes versus landscape controls. These findings provide insight on the hierarchical scaling of local, longitudinal, and landscape processes that drive biogeochemical patterns in stream networks. C1 [McGuire, Kevin J.] Virginia Polytech Inst & State Univ, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. [McGuire, Kevin J.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Torgersen, Christian E.] Univ Washington, Forest & Rangeland Ecosyst Sci Ctr, US Geol Survey, Cascadia Field Stn, Seattle, WA 98195 USA. [Torgersen, Christian E.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Likens, Gene E.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Likens, Gene E.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Buso, Donald C.] Hubbard Brook Forest Stn, Cary Inst Ecosyst Studies, North Woodstock, NH 03262 USA. [Lowe, Winsor H.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Bailey, Scott W.] US Forest Serv, No Res Stn, Hubbard Brook Expt Forest, North Woodstock, NH 03262 USA. RP McGuire, KJ (reprint author), Virginia Polytech Inst & State Univ, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. EM kevin.mcguire@vt.edu; likensg@ecostudies.org RI McGuire, Kevin/E-7770-2010; OI McGuire, Kevin/0000-0001-5751-3956; Bailey, Scott/0000-0002-9160-156X FU National Science Foundation (NSF); Long Term Research in Environmental Biology and Long Term Ecological Research programs; The A.W. Mellon Foundation; NSF [EAR 1014507, DEB 1050459]; NCEAS (NSF) [EF 0553768]; University of California Santa Barbara; State of California FX P. Likens is thanked for help with submission of the manuscript. Financial support for data collection was provided by the National Science Foundation (NSF), including the Long Term Research in Environmental Biology and Long Term Ecological Research programs, and The A.W. Mellon Foundation. Support for K.J.M. and S. W. B. was provided by NSF Grant EAR 1014507. Support for W. H. L. was provided by NSF Grant DEB 1050459. The US Forest Service (Northern Research Station, Newtown Square, PA) operates and maintains the Hubbard Brook Experimental Forest. This manuscript benefited greatly from discussions with the Spatial Statistics for Streams Working Group supported by NCEAS (NSF Grant EF 0553768), University of California Santa Barbara, and the State of California. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 46 TC 13 Z9 13 U1 8 U2 71 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAY 13 PY 2014 VL 111 IS 19 BP 7030 EP 7035 DI 10.1073/pnas.1404820111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AH0GS UT WOS:000335798000067 PM 24753575 ER PT J AU Wang, B Park, B Kwon, Y Xu, B AF Wang, Bin Park, Bosoon Kwon, Yongkuk Xu, Bingqian TI Determining the elastic properties of aptamer-ricin single molecule multiple pathway interactions SO APPLIED PHYSICS LETTERS LA English DT Article ID ATOMIC-FORCE MICROSCOPY; NANOMECHANICAL PROPERTIES; DNA; SPECTROSCOPY; MODELS; BIOMOLECULES; CELLS AB We report on the elastic properties of ricin and anti-ricin aptamer interactions, which showed three stable binding conformations, each of which has its special elastic properties. These different unbinding pathways were investigated by the dynamic force spectroscopy. A series-spring model combining the worm-like-chain model and Hook's law was used to estimate the apparent spring constants of the aptamer and linker molecule polyethylene glycol. The aptamer in its three different unbinding pathways showed different apparent spring constants. The two reaction barriers in the unbinding pathways also influence the apparent spring constant of the aptamer. This special elastic behavior of aptamer was used to distinguish its three unbinding pathways under different loading rates. This method also offered a way to distinguish and discard the non-specific interactions in single molecule experiments. (c) 2014 AIP Publishing LLC. C1 [Wang, Bin; Xu, Bingqian] Univ Georgia, Single Mol Study Lab, Coll Engn & Nanoscale Sci, Athens, GA 30602 USA. [Wang, Bin; Xu, Bingqian] Univ Georgia, Ctr Engn, Athens, GA 30602 USA. [Park, Bosoon] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA. [Kwon, Yongkuk] Anim Plant & Fisheries Quarantine & Inspect Agcy, Avian Dis Div, Anyang, South Korea. RP Wang, B (reprint author), Univ Georgia, Single Mol Study Lab, Coll Engn & Nanoscale Sci, Athens, GA 30602 USA. EM bxu@engr.uga.edu FU National Science Foundation [ECCS 1231967, CBET 1139057] FX We thank National Science Foundation (ECCS 1231967 and CBET 1139057) for financial support of this research. NR 29 TC 1 Z9 1 U1 1 U2 17 PU AMER INST PHYSICS PI MELVILLE PA 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA SN 0003-6951 EI 1077-3118 J9 APPL PHYS LETT JI Appl. Phys. Lett. PD MAY 12 PY 2014 VL 104 IS 19 AR 193702 DI 10.1063/1.4876603 PG 4 WC Physics, Applied SC Physics GA AI5ON UT WOS:000336918600076 ER PT J AU Fox, SE Geniza, M Hanumappa, M Naithani, S Sullivan, C Preece, J Tiwari, VK Elser, J Leonard, JM Sage, A Gresham, C Kerhornou, A Bolser, D McCarthy, F Kersey, P Lazo, GR Jaiswal, P AF Fox, Samuel E. Geniza, Matthew Hanumappa, Mamatha Naithani, Sushma Sullivan, Chris Preece, Justin Tiwari, Vijay K. Elser, Justin Leonard, Jeffrey M. Sage, Abigail Gresham, Cathy Kerhornou, Arnaud Bolser, Dan McCarthy, Fiona Kersey, Paul Lazo, Gerard R. Jaiswal, Pankaj TI De Novo Transcriptome Assembly and Analyses of Gene Expression during Photomorphogenesis in Diploid Wheat Triticum monococcum SO PLOS ONE LA English DT Article ID TERMINATION FACTOR MTERF; AEGILOPS-TAUSCHII; GENOME PROGENITOR; PLANT DEVELOPMENT; DRAFT GENOME; PROTEIN; DNA; ARABIDOPSIS; ANNOTATION; LIGHT AB Background: Triticum monococcum (2n) is a close ancestor of T. urartu, the A-genome progenitor of cultivated hexaploid wheat, and is therefore a useful model for the study of components regulating photomorphogenesis in diploid wheat. In order to develop genetic and genomic resources for such a study, we constructed genome-wide transcriptomes of two Triticum monococcum subspecies, the wild winter wheat T. monococcum ssp. aegilopoides (accession G3116) and the domesticated spring wheat T. monococcum ssp. monococcum (accession DV92) by generating de novo assemblies of RNA-Seq data derived from both etiolated and green seedlings. Principal Findings: The de novo transcriptome assemblies of DV92 and G3116 represent 120,911 and 117,969 transcripts, respectively. We successfully mapped similar to 90% of these transcripts from each accession to barley and similar to 95% of the transcripts to T. urartu genomes. However, only similar to 77% transcripts mapped to the annotated barley genes and similar to 85% transcripts mapped to the annotated T. urartu genes. Differential gene expression analyses revealed 22% more light up-regulated and 35% more light down-regulated transcripts in the G3116 transcriptome compared to DV92. The DV92 and G3116 mRNA sequence reads aligned against the reference barley genome led to the identification of similar to 500,000 single nucleotide polymorphism (SNP) and similar to 22,000 simple sequence repeat (SSR) sites. Conclusions: De novo transcriptome assemblies of two accessions of the diploid wheat T. monococcum provide new empirical transcriptome references for improving Triticeae genome annotations, and insights into transcriptional programming during photomorphogenesis. The SNP and SSR sites identified in our analysis provide additional resources for the development of molecular markers. C1 [Fox, Samuel E.; Geniza, Matthew; Hanumappa, Mamatha; Naithani, Sushma; Sullivan, Chris; Preece, Justin; Elser, Justin; Sage, Abigail; Jaiswal, Pankaj] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Geniza, Matthew] Oregon State Univ, Mol & Cellular Biol Grad Program, Corvallis, OR 97331 USA. [Naithani, Sushma; Sullivan, Chris] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Tiwari, Vijay K.; Leonard, Jeffrey M.; Jaiswal, Pankaj] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Gresham, Cathy] Mississippi State Univ, Inst Gen Biocomp & Biotechnol, Mississippi State, MS 39762 USA. [Kerhornou, Arnaud; Bolser, Dan; Kersey, Paul] European Bioinformat Inst, Cambridge, England. [McCarthy, Fiona] Univ Arizona, Sch Anim & Comparat Biomed Sci, Tucson, AZ USA. [Lazo, Gerard R.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Jaiswal, P (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM jaiswalp@science.oregonstate.edu RI Lazo, Gerard/A-8900-2009; Jaiswal, Pankaj/H-7599-2016 OI Lazo, Gerard/0000-0002-9160-2052; Jaiswal, Pankaj/0000-0002-1005-8383 FU European Commission [283496]; Triticeae Genomics for Sustainable Agriculture project (BBSRC) [BB/J003743/1] FX This work was supported by the laboratory startup funds provided by the Department of Botany and Plant Pathology and College of Agricultural Sciences at Oregon State University to PJ and SN. Research funds were also provided by the Department of Crop and Soil Science, Agricultural Sciences at Oregon State University to JL. MG received Anita S. Summers travel award from the Department of Botany and Plant Pathology at Oregon State University and to present this work at the Plant and Animal Genome Conference. Contributions from International collaborators, PK, DB and AK were supported by the 'transPLANT' project funded by the European Commission within its 7th Framework Programme, under the thematic area 'Infrastructures' (contract #283496) and the Triticeae Genomics for Sustainable Agriculture project (BBSRC: #BB/J003743/1). The funders had no role in the study design, data analysis, or preparation of the manuscript. NR 63 TC 12 Z9 13 U1 2 U2 36 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 12 PY 2014 VL 9 IS 5 AR e96855 DI 10.1371/journal.pone.00996855 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI1ZC UT WOS:000336653300038 PM 24821410 ER PT J AU Oliver, RE Islamovic, E Obert, DE Wise, ML Herrin, LL Hang, A Harrison, SA Ibrahim, A Marshall, JM Miclaus, KJ Lazo, GR Hu, GS Jackson, EW AF Oliver, Rebekah E. Islamovic, Emir Obert, Donald E. Wise, Mitchell L. Herrin, Lauri L. Hang, An Harrison, Stephen A. Ibrahim, Amir Marshall, Juliet M. Miclaus, Kelci J. Lazo, Gerard R. Hu, Gongshe Jackson, Eric W. TI Comparative Systems Biology Reveals Allelic Variation Modulating Tocochromanol Profiles in Barley (Hordeum vulgare L.) SO PLOS ONE LA English DT Article ID RICH FRACTION TRF25; VITAMIN-E ACTION; GENETIC DISSECTION; ALPHA-TOCOPHEROL; HYPERCHOLESTEROLEMIC HUMANS; SPINACH-CHLOROPLASTS; MOLECULAR-BASIS; I-TASSER; TOCOTRIENOL; ANTIOXIDANT AB Tocochromanols are recognized for nutritional content, plant stress response, and seed longevity. Here we present a systems biological approach to characterize and develop predictive assays for genes affecting tocochromanol variation in barley. Major QTL, detected in three regions of a SNP linkage map, affected multiple tocochromanol forms. Candidate genes were identified through barley/ rice orthology and sequenced in genotypes with disparate tocochromanol profiles. Gene-specific markers, designed based on observed polymorphism, mapped to the originating QTL, increasing R 2 values at the respective loci. Polymorphism within promoter regions corresponded to motifs known to influence gene expression. Quantitative PCR analysis revealed a trend of increased expression in tissues grown at cold temperatures. These results demonstrate utility of a novel method for rapid gene identification and characterization, and provide a resource for efficient development of barley lines with improved tocochromanol profiles. C1 [Oliver, Rebekah E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Oliver, Rebekah E.; Islamovic, Emir; Obert, Donald E.; Hang, An; Hu, Gongshe; Jackson, Eric W.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA. [Obert, Donald E.] Limagrain Cereal Seeds, Indiana, PA USA. [Wise, Mitchell L.; Herrin, Lauri L.] USDA ARS, Cereal Crops Res Unit, Madison, WI USA. [Harrison, Stephen A.] Louisiana State Univ, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Ibrahim, Amir] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Marshall, Juliet M.] Univ Idaho Res & Extens, Dept Plant Soil & Entomol Sci, Idaho Falls, ID USA. [Miclaus, Kelci J.] SAS Inst Inc, JMP Genom Dev, Cary, NC USA. [Lazo, Gerard R.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Jackson, Eric W.] Gen Mill Inc, Crop Biosci, Kannapolis, NC USA. RP Jackson, EW (reprint author), Gen Mill Inc, Crop Biosci, Kannapolis, NC USA. EM Rebekah.Oliver@ndsu.edu RI Lazo, Gerard/A-8900-2009 OI Lazo, Gerard/0000-0002-9160-2052 FU United States Department of Agriculture - Agricultural Research Service CRIS project [301] FX This work was supported by the United States Department of Agriculture - Agricultural Research Service CRIS project 301. SAS Institute Inc., provided support in the form of a salary for author KJM, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of the authors are articulated in the "Author Contributions'' section. NR 59 TC 2 Z9 2 U1 3 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 12 PY 2014 VL 9 IS 5 AR e96276 DI 10.1371/journal.pone.0096276 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI1ZC UT WOS:000336653300013 PM 24820172 ER PT J AU Lozano, N Andrade, NA Deng, D Torrents, A Rice, CP McConnell, LL Ramirez, M Millner, PD AF Lozano, Nuria Andrade, Natasha A. Deng, Di Torrents, Alba Rice, Clifford P. McConnell, L. A. U. R. A. L. Ramirez, Mark Millner, Patricia D. TI Fate of microconstituents in biosolids composted in an aerated silage bag SO JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING LA English DT Article DE methyltriclosan; triclosan; Aerated silage bag; triclocarban; PBDEs; biosolids ID POLYBROMINATED DIPHENYL ETHERS; WASTE-WATER TREATMENT; DIFFERENT MOISTURE CONTENTS; PERSONAL CARE PRODUCTS; SEWAGE-TREATMENT; SURFACE WATERS; HUMAN EXPOSURE; POLYCHLORINATED-BIPHENYLS; AEROBIC BIODEGRADATION; ENVIRONMENTAL FATE AB Although most composting studies report pathogen concentrations, little is known about the fate of Endocrine Disruptor Chemicals (EDCs) during composting. In this study, a positively aerated polyethylene bag composting system was filled with a mixture of woodchips and limed biosolids from a large Waste Water Treatment Plant (WWTP) to study the removal efficiency of two different groups of EDCs. Two antibacterial compounds, Triclocarban (TCC) and Triclosan (TCS), and a TCS byproduct, Methyltriclosan (MeTCS), as well as seven congeners of flame retardants known as PBDEs (Polybrominated Diphenyl Ethers) were studied during two phases of composting: 1) a thermophilic phase, in which positive mechanical aeration, pushing air into and through the materials matrix, was conducted for 2 months; and 2) a curing and stabilization phase in which no mechanical aeration was provided and the bag was opened to ambient passive aeration to simulate storage conditions for seven months. Our results showed that while TCC concentrations remained constant, TCS degradation took place during both phases. The degradation of TCS was corroborated by the formation of MeTCS in both phases. The TCS concentrations decreased from 18409 +/- 1,877 to 11955 +/- 288ng g(-1) dry wt. during the thermophilic phase and declined from 11,955 +/- 288 to 7,244 +/- 909. ng g(-1) dry wt. by the end of the curing phase. Thus, slightly greater TCS transformation occurred during the second than during the first (35.1vs. 39.4%). MeTCS concentrations increased from 189.3 +/- 8.6 to 364.6 +/- 72.5ng g(-1) dry wt. during the first phase and reached 589.0 +/- 94.9ng g(-1) dry wt. at the end of the second phase. PBDEs concentrations were below quantification limits for all but two of the congeners analyzed (BDE-47 and BDE-99). PBDE concentrations were measured at the end of the first phase only and were comparable to initial concentrations. C1 [Lozano, Nuria] Univ Cantabria, Dept Water & Environm Sci & Technol, Santander 39012, Cantabria, Spain. [Lozano, Nuria; Rice, Clifford P.; McConnell, L. A. U. R. A. L.] ARS, Environm Management & Byprod Utilizat Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD USA. [Lozano, Nuria; Andrade, Natasha A.; Deng, Di; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Ramirez, Mark] Water & Sewer Author, Dist Columbia, DCWater, Washington, DC USA. [Millner, Patricia D.] ARS, Environm Microbial & Food Safety Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD USA. RP Lozano, N (reprint author), Univ Cantabria, Environm Engn Grp, Dept Water & Environm Sci & Technol, Avda Castros S-N, Santander 39012, Cantabria, Spain. EM nuria.lozano@unican.es FU DC Water and Sewer Authority (DCWater), Washington, DC FX This study was supported by DC Water and Sewer Authority (DCWater), Washington, DC. NR 73 TC 1 Z9 1 U1 0 U2 38 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 520 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1093-4529 EI 1532-4117 J9 J ENVIRON SCI HEAL A JI J. Environ. Sci. Health Part A-Toxic/Hazard. Subst. Environ. Eng. PD MAY 12 PY 2014 VL 49 IS 6 BP 720 EP 730 DI 10.1080/10934529.2014.865461 PG 11 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AB2CX UT WOS:000331601700012 PM 24521417 ER PT J AU Yi, MQ Fu, JD Zhou, LL Gao, H Fan, CG Shao, J Xu, BH Wang, QR Li, JT Huang, GW Lapsley, K Blumberg, JB Chen, CYO AF Yi, Muqing Fu, Jinde Zhou, Lili Gao, Hong Fan, Chenguang Shao, Jing Xu, Baohua Wang, Qirong Li, Juntao Huang, Guangwei Lapsley, Karen Blumberg, Jeffrey B. Chen, C-Y Oliver TI The effect of almond consumption on elements of endurance exercise performance in trained athletes SO JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION LA English DT Article DE Almonds; Exercise performance; Substrate oxidation; Antioxidant defense capacity ID MUSCLE MITOCHONDRIAL BIOGENESIS; TYPE-2 DIABETES-MELLITUS; HIGH-INTENSITY EXERCISE; OXIDATIVE STRESS; FREE-RADICALS; L-ARGININE; POSTPRANDIAL GLYCEMIA; LIPID-PEROXIDATION; NITRIC-OXIDE; RISK-FACTORS AB Background: Almonds are a healthy tree nut food with high nutrient density. Their consumption has been shown to ameliorate oxidative stress, inflammation, etc. The objective of the study was to examine the effect of almonds on elements of endurance exercise performance in trained athletes. Methods: A 10-week crossover, placebo controlled study was conducted. Eight trained male cyclists and two triathletes were randomly assigned to consume 75 g/d whole almonds (ALM) or isocaloric cookies (COK) with equal subject number. They consumed the assigned food for 4 wks and then the alternate food for another 4 wks. They underwent 3 performance tests including 125-min steady status exercise (SS) and 20-min time trial (TT) on an indoor stationary trainer at the start of the study (BL) and at the end of each intervention phase. Venous blood was collected in the morning prior to the performance test for biochemical measurements and finger blood during the test for glucose determination. Carbohydrate and fat oxidation, energy expenditure, and oxygen use were calculated using respiratory gas analysis. Results: ALM increased cycling distance during TT by 1.7 km as compared BL (21.9 vs. 20.2 km, P = 0.053) and COK increased 0.6 km (20.8 vs. 20.2 km, P > 0.05). ALM, but not COK, led to higher CHO and lower fat oxidation and less oxygen consumption during TT than BL (P < 0.05), whereas there was no significant difference in heart rate among BL, ALM and COK. ALM maintained higher blood glucose level after TT than COK (P < 0.05). ALM had higher vitamin E and haemoglobin and lower serum free fatty acid (P < 0.05), slightly elevated serum arginine and nitric oxide and plasma insulin (P > 0.05) than BL, and a higher total antioxidant capacity than COK (P < 0.05). Conclusions: Whole almonds improved cycling distance and the elements related to endurance performance more than isocaloric cookies in trained athletes as some nutrients in almonds may contribute to CHO reservation and utilization and effective oxygen utilization. The results suggest that almonds can be incorporated into diets of those who undertake exercise training for performance improvement. C1 [Yi, Muqing; Fu, Jinde; Zhou, Lili; Gao, Hong; Shao, Jing; Xu, Baohua; Wang, Qirong; Li, Juntao] Natl Inst Sports Med, Ctr Sports Nutr, Beijing 100029, Peoples R China. [Fan, Chenguang] Chinese Baiyi Cycling Team, Beijing 100072, Peoples R China. [Huang, Guangwei; Lapsley, Karen] Almond Board Calif, Modesto, CA 95354 USA. [Blumberg, Jeffrey B.; Chen, C-Y Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr, Antioxidants Res Lab, Boston, MA 02111 USA. RP Yi, MQ (reprint author), Natl Inst Sports Med, Ctr Sports Nutr, 1st Anding Rd, Beijing 100029, Peoples R China. EM Muqingyi@163.com; lilizhou666@163.com FU Almond Board of California FX The study was supported by the Almond Board of California. The authors thank the coaches and physicians for the Chinese Bayi Cycling and Triathlon Team for their support on training and performance test arrangement and dietary information collection. NR 49 TC 0 Z9 0 U1 1 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1550-2783 J9 J INT SOC SPORT NUTR JI J. Int. Soc. Sport Nutr. PD MAY 11 PY 2014 VL 11 AR 18 DI 10.1186/1550-2783-11-18 PG 9 WC Nutrition & Dietetics; Sport Sciences SC Nutrition & Dietetics; Sport Sciences GA AJ2GM UT WOS:000337473800001 PM 24860277 ER PT J AU Mwaliko, E Downing, R O'Meara, W Chelagat, D Obala, A Downing, T Simiyu, C Odhiambo, D Ayuo, P Menya, D Khwa-Otsyula, B AF Mwaliko, Emily Downing, Raymond O'Meara, Wendy Chelagat, Dinah Obala, Andrew Downing, Timothy Simiyu, Chrispinus Odhiambo, David Ayuo, Paul Menya, Diana Khwa-Otsyula, Barasa TI "Not too far to walk": the influence of distance on place of delivery in a western Kenya health demographic surveillance system SO BMC HEALTH SERVICES RESEARCH LA English DT Article DE Global positioning system; Demographic and surveillance system; Maternal health services; Emergency obstetric care; Hotspot analysis; Home/facility births ID MATERNAL MORTALITY; CONTEXT AB Background: Maternal health service coverage in Kenya remains low, especially in rural areas where 63% of women deliver at home, mainly because health facilities are too far away and/or they lack transport. The objectives of the present study were to (1) determine the association between the place of delivery and the distance of a household from the nearest health facility and (2) study the demographic characteristics of households with a delivery within a demographic surveillance system (DSS). Methods: Census sampling was conducted for 13,333 households in the Webuye health and demographic surveillance system area in 2008-2009. Information was collected on deliveries that had occurred during the previous 12 months. Digital coordinates of households and sentinel locations such as health facilities were collected. Data were analyzed using STATA version 11. The Euclidean distance from households to health facilities was calculated using WinGRASS version 6.4. Hotspot analysis was conducted in ArcGIS to detect clustering of delivery facilities. Unadjusted and adjusted odds ratios were estimated using logistic regression models. P-values less than 0.05 were considered significant. Results: Of the 13,333 households in the study area, 3255 (24%) reported a birth, with 77% of deliveries being at home. The percentage of home deliveries increased from 30% to 80% of women living within 2km from a health facility. Beyond 2km, distance had no effect on place of delivery (OR 1.29, CI 1.06-1.57, p = 0.011). Heads of households where women delivered at home were less likely to be employed (OR 0.598, CI 0.43-0.82, p = 0.002), and were less likely to have secondary education (OR 0.50, CI 0.41-0.61, p < 0.0001). Hotspot analysis showed households having facility deliveries were clustered around facilities offering comprehensive emergency obstetric care services. Conclusion: Households where the nearest facility was offering emergency obstetric care were more likely to have a facility delivery, but only if the facility was within 2km of the home. Beyond the 2-km threshold, households were equally as likely to have home and facility deliveries. There is need for further research on other factors that affect the choice of place of delivery, and their relationships with maternal mortality. C1 [Mwaliko, Emily] Moi Univ, Sch Med, Dept Reprod Hlth, Coll Hlth Sci, Eldoret, Kenya. [Downing, Raymond] Moi Univ, Sch Med, Dept Family Med, Coll Hlth Sci, Eldoret, Kenya. [O'Meara, Wendy] Duke Univ, Sch Med, Durham, NC USA. [O'Meara, Wendy] Inst Durham, Duke Global Hlth, Durham, NC USA. [Chelagat, Dinah] Moi Univ, Sch Nursing, Coll Hlth Sci, Eldoret, Kenya. [Obala, Andrew] Moi Univ, Sch Med, Dept Microbiol, Coll Hlth Sci, Eldoret, Kenya. [Downing, Timothy] US Forest Serv, USDA, Santa Fe Natl Forest, Santa Fe, NM 87508 USA. [Simiyu, Chrispinus; Ayuo, Paul] Moi Univ, Sch Med, Dept Med, Coll Hlth Sci, Eldoret, Kenya. [Odhiambo, David] Moi Univ, Coll Hlth Sci, HDSS Program, Eldoret, Kenya. [Menya, Diana] Moi Univ, Sch Publ Hlth, Coll Hlth Sci, Eldoret, Kenya. [Khwa-Otsyula, Barasa] Moi Univ, Sch Med, Dept Surg, Coll Hlth Sci, Eldoret, Kenya. RP Mwaliko, E (reprint author), Moi Univ, Sch Med, Dept Reprod Hlth, Coll Hlth Sci, POB 4606, Eldoret, Kenya. EM waleghwaem@hotmail.com FU Webuye community, Webuye Hospital Staff, the Local Administration, the Enumerators; Moi University VLIR-UOS project FX We thank the Webuye community, Webuye Hospital Staff, the Local Administration, the Enumerators and the Moi University VLIR-UOS project for support. NR 16 TC 7 Z9 7 U1 1 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1472-6963 J9 BMC HEALTH SERV RES JI BMC Health Serv. Res. PD MAY 10 PY 2014 VL 14 AR 212 DI 10.1186/1472-6963-14-212 PG 9 WC Health Care Sciences & Services SC Health Care Sciences & Services GA AI3MR UT WOS:000336766200001 PM 24884489 ER PT J AU Ahmadi, M Ascough, JC DeJonge, KC Arabi, M AF Ahmadi, Mehdi Ascough, James C., II DeJonge, Kendall C. Arabi, Mazdak TI Multisite-multivariable sensitivity analysis of distributed watershed models: Enhancing the perceptions from computationally frugal methods SO ECOLOGICAL MODELLING LA English DT Article DE Morris sensitivity analysis; Formal likelihood function; Hydrology; Water quality; SWAT model ID UNCERTAINTY ANALYSIS; GLOBAL SENSITIVITY; AUTOMATIC CALIBRATION; MULTIOBJECTIVE AUTOCALIBRATION; PARAMETER UNCERTAINTY; ENVIRONMENTAL-MODEL; MULTIPLE OBJECTIVES; HYDROLOGIC-MODELS; CATCHMENT MODELS; GLUE METHODOLOGY AB This paper assesses the impact of different likelihood functions in identifying sensitive parameters of the highly parameterized, spatially distributed Soil and Water Assessment Tool (SWAT) watershed model for multiple variables at multiple sites. The global one-factor-at-a-time (OAT) method of Morris was used for sensitivity analysis of streamflow, combined nitrate (NO3) and nitrite (NO2) fluxes, and total phosphorous (TP) at five gage stations in a primarily agricultural watershed in the Midwestern United States. The Morris method was analyzed for 36 combinations of informal likelihood functions, gage stations, and SWAT model output responses, including relative error mass balance (BIAS), Nash-Sutcliffe efficiency (NSE) coefficient, and root mean square error (RMSE) for peak and low fluxes, and one formal likelihood function that aggregates information content from multiple sites and multiple variables using 65 SWAT parameters. The correlation between sensitivity measures from different likelihood functions was also assessed using the Spearman's rank correlation coefficient. Sensitivity of parameters using different likelihood functions was highly variable, although sensitivity of streamflow and TP showed a high correlation. A stronger correlation between sensitivity of nutrient fluxes at the upstream stations as well as the stations closer to the watershed outlets was evident. Comparison of the combined rank of parameters from informal likelihood functions and the ranks obtained from the formal likelihood function confirmed formal likelihood function ability to effectively identify both sensitive and insensitive parameters with less computational and analysis burden. Uncertainty analysis of the Morris results using bootstrap replications showed that both formal and informal likelihood functions identified sensitive parameters with high confidence. (c) 2014 Elsevier B.V. All rights reserved. C1 [Ahmadi, Mehdi; Arabi, Mazdak] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Ascough, James C., II] USDA ARS, ASRU, Ft Collins, CO 80526 USA. [DeJonge, Kendall C.] USDA ARS, WMRU, Ft Collins, CO 80526 USA. RP Ahmadi, M (reprint author), Texas A&M Univ, Spatial Sci Lab, 1500 Res Pkwy, College Stn, TX 77843 USA. EM ahmadi@tamu.edu; jim.ascough@ars.usda.gov; kendall.dejonge@ars.usda.gov; mazdak.arabi@colostate.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture [2007-51130-03876, 2009-51130-06038] FX This study was funded by U.S. Department of Agriculture-National Institute of Food and Agriculture grants 2007-51130-03876 and 2009-51130-06038. NR 84 TC 5 Z9 5 U1 3 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD MAY 10 PY 2014 VL 279 BP 54 EP 67 DI 10.1016/j.ecolmodel.2014.02.013 PG 14 WC Ecology SC Environmental Sciences & Ecology GA AF8TW UT WOS:000334989500006 ER PT J AU Zhang, T Lichstein, JW Birdsey, RA AF Zhang, Tao Lichstein, Jeremy W. Birdsey, Richard A. TI Spatial and temporal heterogeneity in the dynamics of eastern US forests: Implications for developing broad-scale forest dynamics models SO ECOLOGICAL MODELLING LA English DT Article ID GLOBAL VEGETATION MODEL; UNITED-STATES; NEOTROPICAL FOREST; ECOSYSTEM DYNAMICS; CARBON ALLOCATION; TRACTABLE MODEL; BIOSPHERE MODEL; CANOPY TREE; GROWTH; COMPETITION AB Spatial and temporal environmental heterogeneity is known to play an important role in the dynamics of populations and communities. However, the implications of this heterogeneity for developing and testing regional- to global-scale forest dynamics models are largely unexplored. Predictions from forest dynamics models are typically compared to chronosequences assembled from forest inventory data using the space-for-time substitution approach, which assumes that different-aged stands across space have followed (and will follow) the same dynamics. Often, this assumption is invalid in the presence of spatial and/or temporal heterogeneity. We used the perfect plasticity approximation (PPA) forest dynamics model, parameterized with forest inventory data for >10 forest types in the eastern U.S., to diagnose spatial and temporal heterogeneity in forest dynamics, and to explore how this heterogeneity can affect comparisons between predicted dynamics and chronosequence observations. Our results provided evidence that spatial and temporal heterogeneity are widespread in eastern U.S. forests. Temporal heterogeneity was apparent because species whose observed abundances have increased over recent decades tended to be the same species for which predicted abundance (derived from individual-level growth and mortality rates estimated from the recent decades of inventory data) were greater than observed abundance. Spatial heterogeneity was apparent because species had more competitive parameter estimates (higher growth and/or lower mortality) on inventory plots where they are most abundant, relative to other plots in the same forest type. Spatial and temporal heterogeneity both contributed to mismatches between predicted dynamics and chronosequence observations. Predictions of canopy structure (proportion of individuals in the upper canopy vs. the understory) were well-matched to inventory data, suggesting that the PPA's simple space-filling algorithm was not an important source of error in predicting forest dynamics. (c) 2014 Elsevier B.V. All rights reserved. C1 [Zhang, Tao; Lichstein, Jeremy W.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Birdsey, Richard A.] US Forest Serv, No Res Stn, USDA, Newtown Sq, PA 19073 USA. RP Zhang, T (reprint author), Univ Florida, Dept Biol, POB 118525, Gainesville, FL 32611 USA. EM tz@ufl.edu; jlichstein@ufl.edu; rbirdsey@fs.fed.us FU USDA Forest Service Northern Research Station [11-JV-11242306-059] FX We thank Mark Vanderwel, Trevor Caughlin, and two anonymous reviewers for valuable comments on an earlier draft. We thank Stephen Pacala and Drew Purves for useful discussions. Funding was provided by the USDA Forest Service Northern Research Station (Agreement 11-JV-11242306-059). NR 52 TC 4 Z9 5 U1 1 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD MAY 10 PY 2014 VL 279 BP 89 EP 99 DI 10.1016/j.ecolmodel.2014.02.011 PG 11 WC Ecology SC Environmental Sciences & Ecology GA AF8TW UT WOS:000334989500009 ER PT J AU Boiteau, G Vincent, C Leskey, TC Colpitts, BG MacKinley, P Lee, DH AF Boiteau, Gilles Vincent, Charles Leskey, Tracy C. Colpitts, Bruce G. MacKinley, Pamela Lee, Doo-Hyung TI Impact of Host Plant Connectivity, Crop Border and Patch Size on Adult Colorado Potato Beetle Retention SO PLOS ONE LA English DT Article ID LEPTINOTARSA-DECEMLINEATA COLEOPTERA; HABITAT EDGES; TRAP CROPS; CHRYSOMELIDAE; MOVEMENT; DISPERSAL; POPULATION; FLIGHT; BOUNDARIES; RESPONSES AB Tagged Colorado potato beetles (CPB), Leptinotarsa decemlineata (Say), were released on potato plants, Solanum tuberosum L., and tracked using a portable harmonic radar system to determine the impact of host plant spatial distribution on the tendency of the pest to remain on the colonized host plant or patch. Results confirmed the long residency time on the host plant and showed that close connection of the plant to neighboring plants hastened dispersal between plants. Tracking walking CPB for over 6 h in small potato plots revealed that all types of mixed borders tested (potato/bare ground, potato/timothy and potato/woodland) acted as a strong barrier and retained beetles within the patch. In another experiment in potato patches surrounded by bare ground borders, tracked walking CPB displayed similar behaviour for up to four days. The distribution of turning angles in the CPB walking paths was not uniform and corresponded to beetles following the edge rows of potato patches in response to the crop border barrier or reversing their direction as they reached the end of a row and therefore a border. Patch size had no or little effect on beetle retention in the patch. The relative distribution of counts of tagged beetles detected among small (16 m(2)), medium (64 m(2)) and large size (256 m(2)) patches of potato four days after initial release remained similar to that of numbers released. Even though mixed crop borders were a strong barrier to walking CPB emigrating from potato patches, the departure rate of beetles over time was high. Results suggest that the effect of mixed borders is largely limited to dispersal by walking and does not apply to beetles leaving host patches by flight. The manipulation of crop borders and patch size seem to have limited potential for the management of CPB emigrating from potato fields. C1 [Boiteau, Gilles; MacKinley, Pamela] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB, Canada. [Vincent, Charles] Agr & Agri Food Canada, Hort Res & Dev Ctr, St Jean, PQ, Canada. [Leskey, Tracy C.; Lee, Doo-Hyung] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [Colpitts, Bruce G.] Univ New Brunswick, Dept Elect & Comp Engn, Fredericton, NB, Canada. RP Boiteau, G (reprint author), Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB, Canada. EM gilles.boiteau@agr.gc.ca FU Agriculture and Agri-Food Canada NOI/A base program FX This project was financed by Agriculture and Agri-Food Canada NOI/A base program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 1 Z9 1 U1 1 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 9 PY 2014 VL 9 IS 5 AR e95717 DI 10.1371/journal.pone.0095717 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI4LX UT WOS:000336838000017 PM 24816717 ER PT J AU Behnke, MS Zhang, TP Dubey, JP Sibley, LD AF Behnke, Michael S. Zhang, Tiange P. Dubey, Jitender P. Sibley, L. David TI Toxoplasma gondii merozoite gene expression analysis with comparison to the life cycle discloses a unique expression state during enteric development SO BMC GENOMICS LA English DT Article DE Toxoplasma gondii; Merozoite; Enteric stages; Definitive host; Life cycle; Parasite; Gene expression ID UNITED-STATES; IN-VITRO; ENTEROEPITHELIAL STAGES; PROMOTER ELEMENTS; CYST; INFECTION; HOST; CATS; TRANSCRIPTION; VIRULENCE AB Background: Considerable work has been carried out to understand the biology of tachyzoites and bradyzoites of Toxoplasma gondii in large part due to in vitro culture methods for these stages. However, culturing methods for stages that normally develop in the gut of the definitive felid host, including the merozoite and sexual stages, have not been developed hindering the ability to study a large portion of the parasite's life cycle. Here, we begin to unravel the molecular aspects of enteric stages by providing new data on merozoite stage gene expression. Results: To profile gene expression differences in enteric stages we harvested merozoites from the intestine of infected cats and hybridized mRNA to the Affymetrix Toxoplasma GeneChip. We analyzed the merozoite data in context of the life cycle by comparing it to previously published data for the oocyst, tachyzoite, and bradyzoite stages. Principal component analysis highlighted the unique profile of merozoites, placing them approximately half-way on a continuum between the tachyzoite/bradyzoite and oocyst samples. Prior studies have shown that antibodies to surface antigen one (SAG1) and many dense granule proteins do not label merozoites: our microarray data confirms that these genes were not expressed at this stage. Also, the expression for many rhoptry and microneme proteins was drastically reduced while the expression for many surface antigens was increased at the merozoite stage. Gene Ontology and KEGG analysis revealed that genes involved in transcription/translation and many metabolic pathways were upregulated at the merozoite stage, highlighting unique growth requirements of this stage. To functionally test these predictions, we demonstrated that an upstream promoter region of a merozoite specific gene was sufficient to control expression in merozoites in vivo. Conclusions: Merozoites are the first developmental stage in the coccidian cycle that takes place within the gut of the definitive host. The data presented here describe the global gene expression profile of the merozoite stage and the creation of transgenic parasite strains that show stage-specific expression of reporter genes in the cat intestine. These data and reagents will be useful in unlocking how the parasite senses and responds to the felid gut environment to initiate enteric development. C1 [Behnke, Michael S.; Zhang, Tiange P.; Sibley, L. David] Washington Univ, Sch Med, Dept Mol Biol, St Louis, MO 63110 USA. [Dubey, Jitender P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Behnke, MS (reprint author), Washington Univ, Sch Med, Dept Mol Biol, 660 S Euclid Ave, St Louis, MO 63110 USA. EM mike_behnke@yahoo.com RI Behnke, Michael/R-8839-2016 OI Behnke, Michael/0000-0002-4668-8109 FU NIH [AI059176] FX We would like to thank Joanna Gress of the Functional Genomics Core at Montana State University for her expertise in amplifying, labeling, and hybridizing the mRNA samples to the Toxoplasma gondii Affymetrix microarray and to the Advanced Imaging and Tissue Analysis Core (AITAC) at Washington University School of Medicine for processing the histological samples for immunohistochemistry. We are also grateful to Jennifer Barks for help assistance with parasite culture and to the EuPathDB Project Team for the creation and maintenance of ToxoDB.org. Partially supported by a grant from the NIH (AI059176). NR 58 TC 9 Z9 11 U1 1 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 8 PY 2014 VL 15 AR 350 DI 10.1186/1471-2164-15-350 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AH3ZY UT WOS:000336066300001 PM 24885521 ER PT J AU Feng, BZ Zhu, XP Fu, L Lv, RF Storey, D Tooley, P Zhang, XG AF Feng, Bao-Zhen Zhu, Xiao-Ping Fu, Li Lv, Rong-Fei Storey, Dylan Tooley, Paul Zhang, Xiu-Guo TI Characterization of necrosis-inducing NLP proteins in Phytophthora capsici SO BMC PLANT BIOLOGY LA English DT Article DE Phytophthora capsici; Necrosis-inducing proteins (NLPs); PcNLP genes; Chlorotic or necrotic response; mRNA expression; PcNLPs protein expression ID PLANT PATHOGEN PHYTOPHTHORA; CAROTOVORA SUBSP CAROTOVORA; NEP1-LIKE PROTEINS; GENE FAMILY; FUNCTIONAL-ANALYSIS; FUSARIUM-OXYSPORUM; EFFECTOR PROTEINS; ENCODING NECROSIS; HOST-SPECIFICITY; RXLR EFFECTORS AB Background: Effector proteins function not only as toxins to induce plant cell death, but also enable pathogens to suppress or evade plant defense responses. NLP-like proteins are considered to be effector proteins, and they have been isolated from bacteria, fungi, and oomycete plant pathogens. There is increasing evidence that NLPs have the ability to induce cell death and ethylene accumulation in plants. Results: We evaluated the expression patterns of 11 targeted PcNLP genes by qRT-PCR at different time points after infection by P. capsici. Several PcNLP genes were strongly expressed at the early stages in the infection process, but the expression of other PcNLP genes gradually increased to a maximum at late stages of infection. The genes PcNLP2, PcNLP6 and PcNLP14 showed the highest expression levels during infection by P. capsici. The necrosis-inducing activity of all targeted PcNLP genes was evaluated using heterologous expression by PVX agroinfection of Capsicum annuum and Nicotiana benthamiana and by Western blot analysis. The members of the PcNLP family can induce chlorosis or necrosis during infection of pepper and tobacco leaves, but the chlorotic or necrotic response caused by PcNLP genes was stronger in pepper leaves than in tobacco leaves. Moreover, PcNLP2, PcNLP6, and PcNLP14 caused the largest chlorotic or necrotic areas in both host plants, indicating that these three genes contribute to strong virulence during infection by P. capsici. This was confirmed through functional evaluation of their silenced transformants. In addition, we further verified that four conserved residues are putatively active sites in PcNLP1 by site-directed mutagenesis. Conclusions: Each targeted PcNLP gene affects cells or tissues differently depending upon the stage of infection. Most PcNLP genes could trigger necrotic or chlorotic responses when expressed in the host C. annuum and the non-host N. benthamiana. Individual PcNLP genes have different phytotoxic effects, and PcNLP2, PcNLP6, and PcNLP14 may play important roles in symptom development and may be crucial for virulence, necrosis-inducing activity, or cell death during infection by P. capsici. C1 [Feng, Bao-Zhen; Zhu, Xiao-Ping; Fu, Li; Lv, Rong-Fei; Zhang, Xiu-Guo] Shandong Agr Univ, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China. [Storey, Dylan] Univ Tennessee, Knoxville, TN USA. [Tooley, Paul] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Zhang, XG (reprint author), Shandong Agr Univ, Dept Plant Pathol, 61 Daizong St, Tai An 271018, Shandong, Peoples R China. EM zhxg@sdau.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest of China [201003004, 2013ZX08009003-001-006] FX This research was supported by Special Fund for Agro-scientific Research in the Public Interest of China (201003004) and 2013ZX08009003-001-006. We thank Prof. Brett Tyler for giving comments on this manuscript. Kurt Lamour very kindly uploaded the P. capsici genome sequence (http://img.jgi.doe.gov/cgi-bin/w/main.cgi). NR 72 TC 9 Z9 10 U1 3 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 8 PY 2014 VL 14 AR 126 DI 10.1186/1471-2229-14-126 PG 19 WC Plant Sciences SC Plant Sciences GA AH3AB UT WOS:000335992400001 PM 24886309 ER PT J AU Huda, MS Roh, MS AF Huda, Masud S. Roh, Mark S. TI Growth and flowering response of woody and herbaceous floral crops grown in petroleum-based plastic pots and bioplastic pots containing poultry feather fibers SO SCIENTIA HORTICULTURAE LA English DT Article DE Bioplastic; Feather keratin; Light transmission; Nutrient availability; Root environment; Tissue analysis ID BEGONIA; RESINS; SOIL AB Corylopsis sinensis var. calvescens, Hydrangea serrata, and the Lilium hybrids, 'Triumphator' and 'Mistress' were grown in injection molded bioplastic pots (BP) containing 30% feather fibers (BP 45 pot) and in petroleum-based pots (plastic pot). Temperature in the growing medium adjacent to the pot and the light transmitted through the pot were measured. Distribution of roots at the surface of the growing medium adjacent to the pots was observed. Macro- and micro-elements in the leaf tissue were analyzed, and flowering responses were recorded. Root development of Lilium hybrids grown in BP 45 pots produced a fewer roots to hybrids grown in the plastic pot based on the visual observation. Flowering, leaf size, and nitrogen concentration were not affected by pot types. However, there was a significant increase in shoot length and nitrogen concentration in leaves of Corylopsis and Hydrangea grown in BP 45 pots. The size and color of the leaves was large and darker in Hydrangea and Corylopsis when grown in BP 45 pots. This could result from the vigorous root mass development influenced by an increase in light transmission to the growing medium through BP 45 pots and not by the release of nitrogen from the BP 45 pots. The physical strength of BP 45 pots can be maintained for one year. We conclude that BP 45 pots processed with pellets containing 30% of feather fibers can be used to grow woody and herbaceous ornamental crops. (C) 2014 Elsevier B.V. All rights reserved. C1 [Huda, Masud S.] Inst Hort Res, Washington, DC 20005 USA. [Roh, Mark S.] USDA ARS, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Roh, MS (reprint author), Dankook Univ, Sch Bioresource Sci, Lab Floriculture & Plant Physiol, Cheonan 330714, Chungnam, South Korea. EM marksroh@gmail.com NR 17 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAY 7 PY 2014 VL 170 BP 131 EP 136 DI 10.1016/j.scienta.2014.03.012 PG 6 WC Horticulture SC Agriculture GA AH9LX UT WOS:000336465000018 ER PT J AU Palmer, MV Thacker, TC Waters, WR Robbe-Austerman, S AF Palmer, Mitchell V. Thacker, Tyler C. Waters, W. Ray Robbe-Austerman, Suelee TI Oral Vaccination of White-Tailed Deer (Odocoileus virginianus) with Mycobacterium bovis Bacillus Calmette-Guerin (BCG) SO PLOS ONE LA English DT Article ID UNITED-STATES; PULMONARY TUBERCULOSIS; WILDLIFE RESERVOIR; BRUSHTAIL POSSUMS; MICHIGAN; INFECTION; EFFICACY; IDENTIFICATION; IMMUNIZATION; PROTECTION AB Wildlife reservoirs of Mycobacterium bovis represent serious obstacles to the eradication of tuberculosis from livestock, particularly cattle. In Michigan, USA tuberculous white-tailed deer transmit M. bovis to other deer and cattle. One approach in dealing with this wildlife reservoir is to vaccinate deer, thus interfering with the intraspecies and interspecies transmission cycles. Thirty-three white-tailed deer were assigned to one of two groups; oral vaccination with 1x10(8) colony-forming units of M. bovis BCG Danish (n = 17); and non-vaccinated (n = 16). One hundred eleven days after vaccination deer were infected intratonsilarly with 300 colony-forming units of virulent M. bovis. At examination, 150 days after challenge, BCG vaccinated deer had fewer gross and microscopic lesions, fewer tissues from which M. bovis could be isolated, and fewer late stage granulomas with extensive liquefactive necrosis. Fewer lesions, especially those of a highly necrotic nature should decrease the potential for dissemination of M. bovis within the host and transmission to other susceptible hosts. C1 [Palmer, Mitchell V.; Thacker, Tyler C.; Waters, W. Ray] Agr Res Serv, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. [Robbe-Austerman, Suelee] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, USDA, Ames, IA USA. RP Palmer, MV (reprint author), Agr Res Serv, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM mitchell.palmer@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This study was funded by the United States Department of Agriculture, Agricultural Research Service. The funder had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 52 TC 4 Z9 4 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 7 PY 2014 VL 9 IS 5 AR e97031 DI 10.1371/journal.pone.0097031 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG9HB UT WOS:000335728900129 PM 24804678 ER PT J AU Vorsino, AE Fortini, LB Amidon, FA Miller, SE Jacobi, JD Price, JP Gon, SO Koob, GA AF Vorsino, Adam E. Fortini, Lucas B. Amidon, Fred A. Miller, Stephen E. Jacobi, James D. Price, Jonathan P. Gon, Sam 'Ohukani'ohi'a, III Koob, Gregory A. TI Modeling Hawaiian Ecosystem Degradation due to Invasive Plants under Current and Future Climates SO PLOS ONE LA English DT Article ID SPECIES DISTRIBUTION MODELS; BIOLOGICAL INVASIONS; GEOGRAPHIC DISTRIBUTIONS; RANDOM FORESTS; NICHE; HOTSPOTS; ISLANDS; EQUILIBRIUM; EXPLANATION; INFORMATION AB Occupation of native ecosystems by invasive plant species alters their structure and/or function. In Hawaii, a subset of introduced plants is regarded as extremely harmful due to competitive ability, ecosystem modification, and biogeochemical habitat degradation. By controlling this subset of highly invasive ecosystem modifiers, conservation managers could significantly reduce native ecosystem degradation. To assess the invasibility of vulnerable native ecosystems, we selected a proxy subset of these invasive plants and developed robust ensemble species distribution models to define their respective potential distributions. The combinations of all species models using both binary and continuous habitat suitability projections resulted in estimates of species richness and diversity that were subsequently used to define an invasibility metric. The invasibility metric was defined from species distribution models with <0.7 niche overlap (Warrens I) and relatively discriminative distributions (Area Under the Curve.0.8; True Skill Statistic >0.75) as evaluated per species. Invasibility was further projected onto a 2100 Hawaii regional climate change scenario to assess the change in potential habitat degradation. The distribution defined by the invasibility metric delineates areas of known and potential invasibility under current climate conditions and, when projected into the future, estimates potential reductions in native ecosystem extent due to climate-driven invasive incursion. We have provided the code used to develop these metrics to facilitate their wider use (Code S1). This work will help determine the vulnerability of native-dominated ecosystems to the combined threats of climate change and invasive species, and thus help prioritize ecosystem and species management actions. C1 [Vorsino, Adam E.; Amidon, Fred A.; Miller, Stephen E.] US Fish & Wildlife Serv, Strateg Habitat Conservat Div, Pacific Isl Off, Honolulu, HI 96819 USA. [Fortini, Lucas B.; Jacobi, James D.] US Geol Survey, Pacific Isl Ecosyst Res Ctr, Honolulu, HI USA. [Fortini, Lucas B.] Pacific Isl Climate Change Cooperat, Honolulu, HI USA. [Price, Jonathan P.] Univ Hawaii, Dept Geog, Hilo, HI 96720 USA. [Gon, Sam 'Ohukani'ohi'a, III] Nat Conservancy Hawaii, Honolulu, HI USA. [Koob, Gregory A.] Nat Resources Conservat Serv, USDA, Honolulu, HI USA. RP Vorsino, AE (reprint author), US Fish & Wildlife Serv, Strateg Habitat Conservat Div, Pacific Isl Off, Honolulu, HI 96819 USA. EM Adam_Vorsino@fws.gov RI GON, Stephanie/O-8324-2016; koob, george/P-8791-2016; OI GON, Stephanie/0000-0002-1513-5018; Fortini, Lucas/0000-0002-5781-7295 FU United States Fish and Wildlife Service FX This work was funded by the United States Fish and Wildlife Service. Employees of the Fish and Wildlife Service analyzed the data, prepared the manuscript, and decided to publish in this journal. NR 125 TC 8 Z9 8 U1 6 U2 65 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 7 PY 2014 VL 9 IS 5 AR e95427 DI 10.1371/journal.pone.0095427 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG9HB UT WOS:000335728900018 PM 24805254 ER PT J AU Carey, AN Gomes, SM Shukitt-Hale, B AF Carey, Amanda N. Gomes, Stacey M. Shukitt-Hale, Barbara TI Blueberry Supplementation Improves Memory in Middle-Aged Mice Fed a High-Fat Diet SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE high-fat diet; obesity; blueberry; recognition memory; spatial memory ID BLOOD-BRAIN-BARRIER; ANTIOXIDANT CAPACITY; RATS; ANTHOCYANINS; STRESS; DEFICITS; OBESITY; PERFORMANCE; ACTIVATION; EXPRESSION AB Consuming a high-fat diet may result in behavioral deficits similar to those observed in aging animals. It has been demonstrated that blueberry supplementation can allay age-related behavioral deficits. To determine if supplementation of a high-fat diet with blueberries offers protection against putative high-fat diet-related declines, 9-month-old C57Bl/6 mice were maintained on low-fat (10% fat calories) or high-fat (60% fat calories) diets with and without 4% freeze-dried blueberry powder. Novel object recognition memory was impaired by the high-fat diet; after 4 months on the high-fat diet, mice spent 50% of their time on the novel object in the testing trial, performing no greater than chance performance. Blueberry supplementation prevented recognition memory deficits after 4 months on the diets, as mice on this diet spent 67% of their time on the novel object. After 5 months on the diets, mice consuming the high-fat diet passed through the platform location less often than mice on low-fat diets during probe trials on days 2 and 3 of Morris water maze testing, whereas mice consuming the high-fat blueberry diet passed through the platform location as often as mice on the low-fat diets. This study is a first step in determining if incorporating more nutrient-dense foods into a high-fat diet can allay cognitive dysfunction. C1 [Carey, Amanda N.; Gomes, Stacey M.; Shukitt-Hale, Barbara] Tufts Univ, Human Nutr Res Ctr Aging, ARS, USDA, Boston, MA 02111 USA. [Carey, Amanda N.] Simmons Coll, Dept Psychol, Boston, MA 02115 USA. RP Carey, AN (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, ARS, USDA, Boston, MA 02111 USA. EM amanda.carey@simmons.edu FU USDA Intramural FX Supported by USDA Intramural. NR 44 TC 7 Z9 7 U1 3 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 7 PY 2014 VL 62 IS 18 BP 3972 EP 3978 DI 10.1021/jf404565s PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AH1KH UT WOS:000335878500015 PM 24446769 ER PT J AU Khatibi, PA Berger, G Wilson, J Brooks, WS McMaster, N Griffey, CA Hicks, KB Nghiem, NP Schmale, DG AF Khatibi, Piyum A. Berger, Greg Wilson, Jhanel Brooks, Wynse S. McMaster, Nicole Griffey, Carl A. Hicks, Kevin B. Nghiem, Nhuan P. Schmale, David G., III TI A Comparison of Two Milling Strategies To Reduce the Mycotoxin Deoxynivalenol in Barley SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE barley; hull; dehulling; milling; mycotoxin reduction; deoxynivalenol; starch retention; fermentation; distillers dried grains with solubles (DDGS) ID FUSARIUM HEAD BLIGHT; FUEL ETHANOL COPRODUCTS; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; WHEAT; ZEARALENONE; INFECTION; HULLESS; MAIZE; GRAIN AB Winter barley (Hordeum vulgare L.), a potential feedstock for fuel ethanol production, may be contaminated with the trichothecene mycotoxin deoxynivalenol (DON). DON is a threat to feed and food safety in the United States and may become concentrated during the production of distillers dried grains with solubles (DDGS). DDGS is a coproduct of fuel ethanol production and is increasingly being used as feed for domestic animals. Therefore, new strategies to reduce the threat of DON in DDGS need to be developed and implemented for grain destined for fuel ethanol production. It is known that large concentrations of DON accumulate in the hulls of wheat and barley. Consequently, improved methods are needed to carefully remove the hull from the grain and preserve the starchy endosperm. Whole kernels from five Virginia winter barley genotypes were used to evaluate the abilities of two different milling strategies (roller milling and precision milling (FitzMill)) for their ability to remove the hull-enriched tissue from the kernel while maintaining starch levels and reducing DON levels in the endosperm-enriched tissue. After whole kernels were milled, DON and starch levels were quantified in the hull-enriched fractions and endosperm-enriched fractions. Initial milling experiments demonstrated that the precision mill system (6 min run time) is able to reduce more DON than the roller mill but with higher starch losses. The average percent DON removed from the kernel with the roller mill was 36.7% +/- 5.5 and the average percent DON removed from the dehulled kernel with the precision mill was 85.1% +/- 9.0. Endosperm-enriched fractions collected from the roller mill and precision mill contained starch levels ranging from 49.0% +/- 12.1 to 59.1% +/- 0.5 and 58.5% +/- 1.6 to 65.3% +/- 3.9, respectively. On average, the precision mill removed a mass of 23.1% +/- 6.8 and resulted in starch losses of 9.6% +/- 6.3, but produced an endosperm-enriched fraction with relatively very little average DON (5.5 +/- 2.7 mu g g(-1)). In contrast, on average, the roller mill removed a mass of 12.2% +/- 1.6 and resulted in starch losses of 2.1% +/- 0.5, but produced an endosperm-enriched fraction with high average DON (20.7 +/- 13.5 mu g g(-1)). In a time course precision milling experiment, we tested barley genotypes Nomini, Atlantic, and VA96-44-304 and attempted to reduce the starch loss seen in the first experiment while maintaining low DON concentrations. Decreasing the run time of the precision mill from 5 to 2 min, reduced starch loss at the expense of higher DON concentrations. Aspirated fractions revealed that the precision milled hull-enriched fraction contained endosperm-enriched components that were highly contaminated with DON. This work has important implications for the reduction of mycotoxins such as DON in barley fuel ethanol coproducts and barley enriched animal feeds and human foods. C1 [Khatibi, Piyum A.; McMaster, Nicole; Schmale, David G., III] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Berger, Greg; Hicks, Kevin B.; Nghiem, Nhuan P.] Univ Arkansas, Rice Res & Extens Ctr, Dept Crop Soil & Environm Sci, Stuttgart, AR 72160 USA. [Wilson, Jhanel] ARS, Sustainable Biofuels & Coprod Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Brooks, Wynse S.; Griffey, Carl A.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. RP Schmale, DG (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM dschmale@vt.edu OI Schmale, David/0000-0002-7003-7429 FU Biodesign and Bioprocessing Research Center at Virginia Tech [208-11-110A-012-331-1]; Maryland Grains Producers Utilization Board [10121612]; Virginia Agricultural Council [10183402]; Virginia Small Grains Board [10278306]; United States Wheat and Barley Scab Initiative [07185403] FX This work was supported primarily by a grant to David G. Schmale (D.G.S.), Carl A. Griffey (C.A.G.), and Kevin B. Hicks (K.B.H.) from the Biodesign and Bioprocessing Research Center at Virginia Tech (Project #208-11-110A-012-331-1). Grants to D.G.S. by the Maryland Grains Producers Utilization Board (Proposal #10121612), the Virginia Agricultural Council (Proposal #10183402), the Virginia Small Grains Board (Proposal #10278306) and the United States Wheat and Barley Scab Initiative (Proposal #07185403) also provided support for the work. The conclusions presented here are those of the authors and do not necessarily reflect the views of the United States Department of Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 35 TC 4 Z9 5 U1 3 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 7 PY 2014 VL 62 IS 18 BP 4204 EP 4213 DI 10.1021/jf501208x PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AH1KH UT WOS:000335878500043 PM 24735088 ER PT J AU Alcala-Diaz, JF Delgado-Lista, J Perez-Martinez, P Garcia-Rios, A Marin, C Quintana-Navarro, GM Gomez-Luna, P Camargo, A Almaden, Y Caballero, J Tinahones, FJ Ordovas, JM Perez-Jimenez, F Lopez-Miranda, J AF Alcala-Diaz, Juan F. Delgado-Lista, Javier Perez-Martinez, Pablo Garcia-Rios, Antonio Marin, Carmen Quintana-Navarro, Gracia M. Gomez-Luna, Purificacion Camargo, Antonio Almaden, Yolanda Caballero, Javier Tinahones, Francisco J. Ordovas, Jose M. Perez-Jimenez, Francisco Lopez-Miranda, Jose TI Hypertriglyceridemia Influences the Degree of Postprandial Lipemic Response in Patients with Metabolic Syndrome and Coronary Artery Disease: From the Cordioprev Study SO PLOS ONE LA English DT Article ID CARDIOVASCULAR-DISEASE; NONFASTING TRIGLYCERIDES; OXIDATIVE STRESS; LIPID-METABOLISM; HEART-DISEASE; RISK; MEN; ATHEROSCLEROSIS; METAANALYSIS; DIETARY AB Objective: To determine whether metabolic syndrome traits influence the postprandial lipemia response of coronary patients, and whether this influence depends on the number of MetS criteria. Materials and Methods: 1002 coronary artery disease patients from the CORDIOPREV study were submitted to an oral fat load test meal with 0.7 g fat/ kg body weight (12% saturated fatty acids, 10% polyunsaturated fatty acids, 43% monounsaturated fatty acids), 10% protein and 25% carbohydrates. Serial blood test analyzing lipid fractions were drawn at 0, 1, 2, 3 and 4 hours during the postprandial state. Total and incremental area under the curves of the different postprandial parameters were calculated following the trapezoid rule to assess the magnitude of change during the postprandial state Results: Postprandial lipemia response was directly related to the presence of metabolic syndrome. We found a positive association between the number of metabolic syndrome criteria and the response of postprandial plasma triglycerides (p< 0.001), area under the curve of triglycerides (p<0.001) and incremental area under the curve of triglycerides (p<0.001). However, the influence of them on postprandial triglycerides remained statistically significant only in those patients without basal hypertriglyceridemia. Interestingly, in stepwise multiple linear regression analysis with the AUC of triglycerides as the dependent variable, only fasting triglycerides, fasting glucose and waist circumference appeared as significant independent (P<0.05) contributors. The multiple lineal regression (R) was 0.77, and fasting triglycerides showed the greatest effect on AUC of triglycerides with a standardized coefficient of 0.75. Conclusions: Fasting triglycerides are the major contributors to the postprandial triglycerides levels. MetS influences the postprandial response of lipids in patients with coronary heart disease, particularly in non-hypertriglyceridemic patients. C1 [Alcala-Diaz, Juan F.; Delgado-Lista, Javier; Perez-Martinez, Pablo; Garcia-Rios, Antonio; Marin, Carmen; Quintana-Navarro, Gracia M.; Gomez-Luna, Purificacion; Camargo, Antonio; Almaden, Yolanda; Perez-Jimenez, Francisco; Lopez-Miranda, Jose] Univ Cordoba, Hosp Univ Reina Sofia, Inst Maimonides Invest Biomed Cordoba IMIBIC, Unidad Lipidos & Arteriosclerosis,Dept Med, Cordoba, Spain. [Alcala-Diaz, Juan F.; Delgado-Lista, Javier; Perez-Martinez, Pablo; Garcia-Rios, Antonio; Marin, Carmen; Gomez-Luna, Purificacion; Camargo, Antonio; Almaden, Yolanda; Perez-Jimenez, Francisco; Lopez-Miranda, Jose] Inst Salud Carlos III, Ctr Invest Biomed Red Fisiopatol Obesidad & Nutr, Madrid, Spain. [Caballero, Javier] Hosp Univ Reina Sofia, Dept Anal Clin, Cordoba, Spain. [Tinahones, Francisco J.] Inst Salud Carlos III, CIBEROBN, Hosp Virgen Victoria, Malaga, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. RP Lopez-Miranda, J (reprint author), Univ Cordoba, Hosp Univ Reina Sofia, Inst Maimonides Invest Biomed Cordoba IMIBIC, Unidad Lipidos & Arteriosclerosis,Dept Med, Cordoba, Spain. EM jlopezmir@uco.es RI Camargo, Antonio/G-9720-2015; Alcala-Diaz, Juan Francisco/F-5329-2016; OI Camargo, Antonio/0000-0002-0415-4184; Alcala-Diaz, Juan Francisco/0000-0002-4572-3611; Perez-Jimenez, Francisco/0000-0001-7499-7681; Perez Martinez, Pablo/0000-0001-7716-8117; Perez Jimenez, Francisco/0000-0001-9808-1280 FU Fundacion Patrimonio Comunal Olivarero; Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca; Consejeria de Innovacion, Ciencia y Empresa); Diputaciones de Jaen y Cordoba; Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Ministerio de Ciencia e Innovacion [AGL2009-122270, FIS PI10/01041, PI10/02412]; Ministerio de Economia y Competitividad [AGL2012/39615]; Consejeria de Economia; Proyectos de Investigacion de Excelencia; Junta de Andalucia [AGR922, PI0193/09]; Consejeria de Salud; Consejeria de Innovacion Ciencia y Empresa [CVI-7450]; ISCIII (Programa Rio-Hortega); Nicolas Monardes Programme; Consejeria de Salud-SAS (Junta de Andalucia); [0118/08]; [PI-0252/09]; [PI-0058/10] FX The CORDIOPREV study is supported from Fundacion Patrimonio Comunal Olivarero, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, Medio Rural y Marino, Gobierno de Espana Also supported in part by research grants from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to J L-M, FIS PI10/01041 to P P-M, PI10/02412 to F P-J); Ministerio de Economia y Competitividad (AGL2012/39615 to J L-M); Consejeria de Economia, Innovacion y Ciencia, Proyectos de Investigacion de Excelencia, Junta de Andalucia (AGR922 to F P-J); Consejeria de Salud, Junta de Andalucia (PI0193/09 to J L-M), 0118/08 to F F-J, PI-0252/09 to J D-L, and PI-0058/10 to P P-M; Consejeria de Innovacion Ciencia y Empresa (CVI-7450 to J L-M. JF Alcala-Diaz is supported by a research contract of ISCIII (Programa Rio-Hortega). Y Almaden is a senior researcher supported by the Nicolas Monardes Programme, Consejeria de Salud-SAS (Junta de Andalucia). The CIBEROBN is an initiative of the Instituto de Salud Carlos III, Madrid, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 11 Z9 11 U1 0 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 6 PY 2014 VL 9 IS 5 AR e96297 DI 10.1371/journal.pone.0096297 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AJ9KS UT WOS:000338029800050 PM 24802225 ER PT J AU Dungan, RS AF Dungan, Robert Stephen TI Estimation of Infectious Risks in Residential Populations Exposed to Airborne Pathogens During Center Pivot Irrigation of Dairy Wastewaters SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID MOUTH-DISEASE VIRUS; CLASS-B BIOSOLIDS; SEWAGE-TREATMENT PLANTS; WATER TREATMENT PLANTS; ESCHERICHIA-COLI O157; LAND APPLICATION; WASTE-WATER; MICROBIAL AEROSOLS; SALMONELLA-TYPHIMURIUM; ZOONOTIC PATHOGENS AB In the western United States where dairy wastewaters are commonly land applied, there are concerns over individuals being exposed to airborne pathogens. In response, a quantitative microbial risk assessment (QMRA) was performed to estimate infectious risks after inhalation exposure of pathogens aerosolized during center pivot irrigation of diluted dairy wastewaters. The dispersion of pathogens (Campylobacter jejuni, Escherichia coli O157:H7, non-O157 E. coli, Listeria monocytogenes, and Salmonella spp.) was modeled using the atmospheric dispersion model, AERMOD. Pathogen concentrations at downwind receptors were used to calculate infectious risks during one-time (1, 8, and 24 h) and multiday (7 d at 1 h d(-1)) exposure events using a beta-Poisson dose-response model. This assessment considered risk of infection in residential populations that were 1 to 10 km from a center pivot operation. In the simulations, infectious risks were estimated to be the greatest in individuals closest to the center pivot, as a result of a higher pathogen dose. On the basis of the results from this QMRA, it is recommended that wastewaters only be applied during daylight hours when inactivation and dilution of airborne pathogens is highest. Further refinement of the dispersion and dose-response models should be considered to increase the utility of this QMRA. C1 USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Dungan, RS (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. EM robert.dungan@ars.usda.gov NR 76 TC 7 Z9 7 U1 5 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD MAY 6 PY 2014 VL 48 IS 9 BP 5033 EP 5042 DI 10.1021/es405693v PG 10 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AG9DY UT WOS:000335720100048 PM 24697271 ER PT J AU Ito, T Wisseman, RW Morse, JC Colbo, MH Weaver, JS AF Ito, Tomiko Wisseman, Robert W. Morse, John C. Colbo, Murray H. Weaver, John S., III TI The genus Palaeagapetus Ulmer (Trichoptera, Hydroptilidae, Ptilocolepinae) in North America SO ZOOTAXA LA English DT Article DE adult; pupa; larva; case; food; habitat; annual life cycle; distribution ID MOUNTAINS-NATIONAL-PARK; CADDISFLIES TRICHOPTERA; WEST-VIRGINIA; EPHEMEROPTERA; PLECOPTERA; CHECKLIST; RECORDS; STONEFLIES; STREAM; TRAP AB The genus Palaeagapetus Ulmer (Trichoptera, Hydroptilidae, Ptilocolepinae) is revised in North America. Descriptions of the western species, P. nearcticus Banks 1938, are provided with the first descriptions of the female, pupa, larva, egg and case and with notes on food, habitat and annual life cycle. The male and female of the eastern species, P. celsus Ross 1936, are described or redescribed with some ecological notes. Distributions of the two species are summarized. C1 [Ito, Tomiko] Hokkaido Aquat Biol, Hokkaido, Hokkaido 0611434, Japan. [Wisseman, Robert W.] Aquat Biol Associates Inc, Corvallis, OR 97330 USA. [Morse, John C.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Colbo, Murray H.] Acad Univ, ACER, SFS Taxon Certificat Program, Wolfville, NS B4P 2R6, Canada. [Weaver, John S., III] PPQ, APHIS, USDA, N Charleston, SC 29406 USA. RP Ito, T (reprint author), Hokkaido Aquat Biol, Hakuyo Cho 3-3-5, Hokkaido, Hokkaido 0611434, Japan. EM tobikera@siren.ocn.ne.jp NR 62 TC 0 Z9 0 U1 0 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAY 6 PY 2014 VL 3794 IS 2 BP 201 EP 221 PG 21 WC Zoology SC Zoology GA AG8CB UT WOS:000335645200001 PM 24870319 ER PT J AU Minocha, R Majumdar, R Minocha, SC AF Minocha, Rakesh Majumdar, Rajtilak Minocha, Subhash C. TI Polyamines and abiotic stress in plants: a complex relationship SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE arginine; biochemical markers; gamma-aminobutyric acid; glutamate; ornithine; proline; reactive oxygen species; stress priming ID S-ADENOSYLMETHIONINE DECARBOXYLASE; CHRONIC NITROGEN ADDITIONS; BROOK EXPERIMENTAL FOREST; NORTHERN HARDWOOD FOREST; ABIES L KARST; ARABIDOPSIS-THALIANA; HARVARD FOREST; DROUGHT TOLERANCE; FOLIAR CHEMISTRY; HUBBARD BROOK AB The physiological relationship between abiotic stress in plants and polyamines was reported more than 40 years ago. Ever since there has been a debate as to whether increased polyamines protect plants against abiotic stress (e.g., due to their ability to deal with oxidative radicals) or cause damage to them (perhaps due to hydrogen peroxide produced by their catabolism). The observation that cellular polyamines are typically elevated in plants under both short-term as well as long-term abiotic stress conditions is consistent with the possibility of their dual effects, i.e., being protectors from as well as perpetrators of stress damage to the cells. The observed increase in tolerance of plants to abiotic stress when their cellular contents are elevated by either exogenous treatment with polyamines or through genetic engineering with genes encoding polyamine biosynthetic enzymes is indicative of a protective role for them. However, through their catabolic production of hydrogen peroxide and acrolein, both strong oxidizers, they can potentially be the cause of cellular harm during stress. In fact, somewhat enigmatic but strong positive relationship between abiotic stress and foliar polyamines has been proposed as a potential biochemical marker of persistent environmental stress in forest trees in which phenotypic symptoms of stress are not yet visible. Such markers may help forewarn forest managers to undertake amelioration strategies before the appearance of visual symptoms of stress and damage at which stage it is often too late for implementing strategies for stress remediation and reversal of damage. This review provides a comprehensive and critical evaluation of the published literature on interactions between abiotic stress and polyamines in plants, and examines the experimental strategies used to understand the functional significance of this relationship with the aim of improving plant productivity, especially under conditions of abiotic stress. C1 [Minocha, Rakesh] US Forest Serv, No Res Stn, Durham, NH USA. [Majumdar, Rajtilak] USDA ARS, Geneva, NY USA. [Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. RP Minocha, SC (reprint author), Univ New Hampshire, Dept Biol Sci, Rudman Hall,46 Coll Rd, Durham, NH 03824 USA. EM sminocha@unh.edu FU New Hampshire Agricultural Experiment Station; United States Department of Agriculture (NRI) [2002-35318-12674]; US Forest Service Northern Research Station; College of Life Sciences and Agriculture; University of New Hampshire Graduate School FX The authors are grateful to Stephanie Long and several graduate students at UNH for help in the original research presented here. This review was prompted by discussions with numerous undergraduate and graduate students in several courses taught by Subhash C. Minocha. The New Hampshire Agricultural Experiment Station, the United States Department of Agriculture (NRI award 2002-35318-12674), the US Forest Service Northern Research Station, the College of Life Sciences and Agriculture, and the University of New Hampshire Graduate School, have provided partial support for the research in the authors labs over the years. The authors also thank the reviewers for useful suggestions for improving the manuscript. NR 166 TC 56 Z9 57 U1 11 U2 123 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 5 PY 2014 VL 5 AR 175 DI 10.3389/fpls.2014.00175 PG 17 WC Plant Sciences SC Plant Sciences GA AI2XD UT WOS:000336721500001 PM 24847338 ER PT J AU Vogeler, JC Hudak, AT Vierling, LA Evans, J Green, P Vierling, KIT AF Vogeler, Jody C. Hudak, Andrew T. Vierling, Lee A. Evans, Jeffrey Green, Patricia Vierling, Kern I. T. TI Terrain and vegetation structural influences on local avian species richness in two mixed-conifer forests SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Forest birds; LiDAR; Landsat; Species richness modeling; Avian nesting guilds; Predictive maps ID BIRD COMMUNITIES; LIDAR-DATA; MANAGED FORESTS; AIRBORNE LIDAR; HABITAT; DIVERSITY; BIODIVERSITY; USA; ENVIRONMENTS; PRODUCTIVITY AB Using remotely-sensed metrics to identify regions containing high animal diversity and/or specific animal species or guilds can help prioritize forest management and conservation objectives across actively managed landscapes. We predicted avian species richness in two mixed conifer forests, Moscow Mountain and Slate Creek, containing different management contexts and located in north-central Idaho. We utilized general linear models and an AIC model selection approach to examine the relative importance of a wide range of remotely-sensed ecological variables, including LiDAR-derived metrics of vertical and horizontal structural heterogeneities of both vegetation and terrain, and Landsat-derived vegetation reflectance indices. We also examined the relative importance of these remotely sensed variables in predicting nesting guild distributions of ground/understory nesters, midupper canopy nesters, and cavity nesters. All top models were statistically significant, with adjusted R(2)s ranging from 0.05 to 0.42. Regardless of study area, the density of the understory was positively associated with total species richness and the ground/understory nesting guild. However, the relative importance of ecological predictors generally differed between the study areas and among the nesting guilds. For example, for mid-upper canopy nester richness, the best predictors at Moscow Mountain included height variability and canopy density whereas at Slate Creek they included slope, elevation, patch diversity and height variability. Topographic variables were not found to influence species richness at Moscow Mountain but were strong predictors of avian species richness at the higher elevation Slate Creek, where species richness decreased with increasing slope and elevation. A variance in responses between focal areas suggests that we expand such studies to determine the relative importance of different factors in determining species richness. It is also important to note that managers using predictive maps should realize that models from one region may not adequately represent communities in other areas. (C) 2014 Elsevier Inc. All rights reserved. C1 [Vogeler, Jody C.; Vierling, Kern I. T.] Univ Idaho, Dept Fish & Wildlife Resources, Moscow, ID 83844 USA. [Hudak, Andrew T.] USDA, Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Vierling, Lee A.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Evans, Jeffrey] Nature Conservancy, Cent Sci, Ft Collins, CO 80524 USA. [Evans, Jeffrey] Univ Wyoming, Laramie, WY 82070 USA. [Green, Patricia] Nez Perce Natl Forests Supervisors Off, Grangeville, ID 83530 USA. RP Vogeler, JC (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM jody.vogeler@oregonstate.edu RI Vierling, Lee/E-6428-2010; Vierling, Kerri/N-6653-2016 OI Vierling, Lee/0000-0001-5344-1983; FU National Gap Analysis Program [08HQAG0123]; US Forest Service; Palouse Audubon Society FX We would like to thank the National Gap Analysis Program (grant number: 08HQAG0123), the US Forest Service, and the Palouse Audubon Society for funding. This is contribution 1080 of the University of Idaho Forest, Wildlife and Range Experiment Station. NR 55 TC 15 Z9 15 U1 1 U2 38 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAY 5 PY 2014 VL 147 BP 13 EP 22 DI 10.1016/j.rse.2014.02.006 PG 10 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AH5QR UT WOS:000336186400002 ER PT J AU Xie, JL Hse, CY Shupe, TF Qi, JQ Pan, H AF Xie, Jiulong Hse, Chung-Yun Shupe, Todd F. Qi, Jinqiu Pan, Hui TI Liquefaction Behaviors of Bamboo Residues in a Glycerol-Based Solvent Using Microwave Energy SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE bamboo liquefaction; glycerol; microwave; FTIR; SEM ID X-RAY-DIFFRACTION; LIQUEFIED WOOD; POLYHYDRIC ALCOHOLS; POLYURETHANE FOAMS; CRUDE GLYCEROL; BIO-OIL; CELLULOSE; SPECTROSCOPY; FEEDSTOCKS; PRODUCTS AB Liquefaction of bamboo was performed in glycerol-methanol as co-solvent using microwave energy and was evaluated by characterizing the liquefied residues. High efficiency conversion of bamboo was achieved under mild reaction conditions. Liquefaction temperature and time interacted to affect the liquefaction reaction. Fourier transform infrared analyzes of the residues indicated that hemicellulose and lignin could easily undergo recondensation. Thermogravimetric analysis results showed that residues obtained at higher temperature/longer reaction time displayed a superior thermal stability as compared to those obtained from mild conditions. Moreover, significant differences were observed in the morphology and structures of residues from different liquefaction conditions according to scanning electron microscopy images. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40207. C1 [Xie, Jiulong; Qi, Jinqiu] Sichuan Agr Univ, Coll Forestry, Yaan 625014, Sichuan, Peoples R China. [Hse, Chung-Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resource, Baton Rouge, LA 70803 USA. [Pan, Hui] Louisiana State Univ, Ctr Agr, Calhoun Res Stn, Calhoun, LA 71225 USA. RP Qi, JQ (reprint author), Sichuan Agr Univ, Coll Forestry, Yaan 625014, Sichuan, Peoples R China. EM qijinqiu2005@aliyun.com NR 31 TC 10 Z9 10 U1 3 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD MAY 5 PY 2014 VL 131 IS 9 DI 10.1002/app.40207 PG 8 WC Polymer Science SC Polymer Science GA AF3GL UT WOS:000334599600031 ER PT J AU Zhang, ML Hao, XL Sanderson, SC Vyacheslav, BV Sukhorukov, AP Zhang, X AF Zhang, Mingli Hao, Xiaoli Sanderson, Stewart C. Vyacheslav, Byalt V. Sukhorukov, Alexander P. Zhang, Xia TI Spatiotemporal evolution of Reaumuria (Tamaricaceae) in Central Asia: insights from molecular biogeography SO PHYTOTAXA LA English DT Article DE Reaumuria; Central Asia; molecular clock; evolution; EOT; QTP ID DISPERSAL-VICARIANCE ANALYSIS; OLIGOCENE CLIMATE TRANSITION; SPECIES DIVERGENCE TIMES; TIBETAN PLATEAU UPLIFT; FOSSIL CALIBRATIONS; BAYESIAN-ESTIMATION; GEOGRAPHIC RANGE; NUCLEAR RDNA; MIOCENE; PHYLOGENY AB Reaumuria is an arid adapted genus with a distribution center in Central Asia; its evolution and dispersal is investigated in this paper. Eighteen species of Reaumuria and nine species of two other genera in the Tamaricaceae, Tamarix and Myricaria, were sampled, and four markers ITS, rps16, psbB-psbH, and trnL-trnF were sequenced. The reconstructed phylogenetic tree is fundamentally consistent with previous morphological classification, except that R. soongorica, sometimes considered to be a separate genus or subgenus, is completely nested within Reaumuria. The ancestral area of the genus is suggested to be western Central Asia, and distributions in the Iran-Mediterranean area and the Tianshan and Pamir-Alai mountains are inferred as dispersals. Westward dispersals to the Iran-Mediterranean were ancient Oligocene to Miocene, whereas dispersals eastward were recent. The spatiotemporal evolution of Reaumuria is used as a link to abiotic paleoclimatic and geological events, in particular, increased aridity beginning at the Eocene-Oligocene Transition (EOT), and as a result of uplift of the Himalayas and Qinghai-Tibet Plateau (QTP). The diversification of the two sections (22.51-19.78 Ma) suggests a response to increasing aridification in response to QTP uplift and expansion. C1 [Zhang, Mingli; Hao, Xiaoli] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. [Zhang, Mingli] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Hao, Xiaoli; Zhang, Xia] Shihezi Univ, Sch Life Sci, Shihezi 832003, Peoples R China. [Sanderson, Stewart C.] US Forest Serv, Shrub Sci Lab, Intermt Res Stn, Provo, UT 84601 USA. [Vyacheslav, Byalt V.] Russian Acad Sci, VL Komarov Bot Inst, RU-197376 St Petersburg, Russia. [Sukhorukov, Alexander P.] Moscow MV Lomonosov State Univ, Fac Biol, Dept Higher Plants, Moscow 119234, Russia. RP Zhang, ML (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China. EM zhangml@ibcas.ac.cn RI Sukhorukov, Alexander/D-2084-2013 OI Sukhorukov, Alexander/0000-0003-2220-826X FU China National Key Basic Research Program [2014CB954201]; CAS Important Direction for Knowledge Innovation Project [KZCX2-EW-305]; CAS visiting professorship for senior international scientists [2012T1Z0020]; Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences FX We are grateful to staffs of herbaria in the Komarov Botanical Institute, Russian Academy of Sciences (St. Petersburg); Moscow University (Moscow); Institute of Botany (Beijing); Northwest Institute of Plateau Biology (Xining); Main Botanical Garden, Russian Academy of Sciences (Moscow); Xinjiang Institute of Ecology and Geography (Urumqi), Chinese Academy of Sciences; and Shihezi University (Shihezi) for their help in our convenient checking of specimens. This study is financially supported by China National Key Basic Research Program (2014CB954201), CAS Important Direction for Knowledge Innovation Project (KZCX2-EW-305), CAS visiting professorship for senior international scientists (2012T1Z0020), and Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences. NR 62 TC 2 Z9 2 U1 2 U2 16 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD MAY 5 PY 2014 VL 167 IS 1 BP 89 EP 103 PG 15 WC Plant Sciences SC Plant Sciences GA AG5EY UT WOS:000335443400005 ER PT J AU Bidlack, AL Benda, LE Miewald, T Reeves, GH McMahan, G AF Bidlack, Allison L. Benda, Lee E. Miewald, Tom Reeves, Gordon H. McMahan, Gabriel TI Identifying Suitable Habitat for Chinook Salmon across a Large, Glaciated Watershed SO TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY LA English DT Article ID JUVENILE PACIFIC SALMON; GLACIER-FED RIVERS; LANDSCAPE CHARACTERISTICS; COHO SALMON; STEELHEAD; SELECTION; STREAM; USA; POPULATIONS; MANAGEMENT AB Ecosystem management requires information on habitat suitability across broad scales; however, comprehensive environmental surveys in remote areas are often impractical and expensive to carry out. Intrinsic Potential (IP) models provide a means to identify on a broad scale those portions of the landscape that can provide essential habitat for various freshwater fish species. These models are derived from watershed patterns and processes that are persistent and not readily affected by human activities. We developed an IP model for rearing habitat of Chinook Salmon throughout the Copper River watershed (63,000km(2)) in southcentral Alaska, utilizing digital elevation models, expert opinion, and field surveys. Our model uses three variables-mean annual flow, gradient, and glacial influence-and adequately predicts where probable habitat for juvenile Chinook Salmon occurs across this large landscape. This model can help resource managers map critical habitat for salmon throughout the Copper River watershed, direct field research to appropriate stream reaches, and assist managers in prioritizing restoration actions, such as culvert replacement. Intrinsic Potential modeling is broadly applicable to other salmonid species and geographies and may inform future work on the ecological impacts of climate change in polar and subpolar river systems. Received August 23, 2013; accepted December 20, 2013 C1 [Bidlack, Allison L.; McMahan, Gabriel] Ecotrust, Portland, OR 97209 USA. [Benda, Lee E.] Earth Syst Inst, Mt Shasta, CA 96067 USA. [Miewald, Tom] US Fish & Wildlife Serv, Portland, OR 97232 USA. [Reeves, Gordon H.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Bidlack, AL (reprint author), Univ Alaska Southeast, Alaska Coastal Rainforest Ctr, 11120 Glacier Highway, Juneau, AK 99801 USA. EM allison.bidlack@uas.alaska.edu FU Gordon and Betty Moore Foundation FX This work was supported by funding from the Gordon and Betty Moore Foundation. We thank the Alaska Department of Fish and Game and the Native Village of Eyak for field assistance and survey data. Thanks also to E. Veach and M. Mc-Cormick, Wrangell St. Elias National Park and Preserve, for National Park Service survey data. This work would not have been possible without our many field assistants, particularly B. Hansen, J. Fisher, and C. Somerville. M. Goslin, A. Fenix, and N. Lyman helped with model building and GIS analysis. We also thank M. Somerville for help with field logistics and R. Brenner for assistance with the figures. C. Sergeant and two anonymous reviewers provided helpful comments on the manuscript. NR 38 TC 4 Z9 4 U1 1 U2 17 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 520 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0002-8487 EI 1548-8659 J9 T AM FISH SOC JI Trans. Am. Fish. Soc. PD MAY 4 PY 2014 VL 143 IS 3 BP 689 EP 699 DI 10.1080/00028487.2014.880739 PG 11 WC Fisheries SC Fisheries GA AG1YE UT WOS:000335211300005 ER PT J AU Moran, PJ Goolsby, JA AF Moran, Patrick J. Goolsby, John A. TI Effect of nitrogen fertilization on growth of Arundo donax and on rearing of a biological control agent, the shoot gall-forming wasp Tetramesa romana SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE biological weed control; gall wasp; mass production; giant reed; plant vigor; resource allocation ID RIO-GRANDE BASIN; GIANT REED; WATER-HYACINTH; HYMENOPTERA EURYTOMIDAE; MELALEUCA-QUINQUENERVIA; EICHHORNIA-CRASSIPES; SOUTHERN CALIFORNIA; PISTIA-STRATIOTES; BIOCONTROL AGENT; RIPARIAN PLANT AB The shoot tip-galling wasp Tetramesa romana Walker (Hymenoptera: Eurytomidae) has been released for biological control of giant reed or arundo (Arundo donax L.) (Poaceae), an invasive grass in the USA and Mexico. The role of urea fertilization to improve plant-based mass-rearing was examined. In a greenhouse study, rhizomes were fertilized with urea pellets at rates equivalent to 1000 kg (low), 2000 kg (moderate), and 4000 (high) kg N per ha(-1). Total nitrogen content of ungalled stems was significantly 0.60-0.65% higher under low and moderate fertilization compared to unfertilized pots, and shoot water content was elevated 3-4% at all urea levels. Moderate fertilization significantly (by 1.4-fold) increased the relative growth rate of all shoots in pots, but did not affect final dry biomass. Fertilization did not affect number and duration of probing events by females. The percentage of shoots colonized by wasps that were galled, progeny production per shoot and per female, and emergent wasp size were not affected. However, average generation time (adult to adult) of emergent wasps was 4-5 days shorter on shoots in pots under moderate and high urea fertilization. After a four-week wasp emergence period, only 3-9% of progeny remained in fertilized shoots, while 21% of progeny remained inside unfertilized shoots. In field plots, fertilization did not affect gall density per m shoot length or per female released. Urea fertilization increased the efficiency of greenhouse rearing of the arundo wasp and availability of adults for release, even without direct effects on gall production. C1 [Moran, Patrick J.] USDA ARS, EIWRU, Albany, CA 94710 USA. [Goolsby, John A.] USDA ARS, Knipling Bushland Livestock Insects Res Lab, Cattle Fever Tick Res Lab, Edinburg, TX USA. RP Moran, PJ (reprint author), USDA ARS, EIWRU, Albany, CA 94710 USA. EM Patrick.Moran@ars.usda.gov NR 66 TC 5 Z9 6 U1 0 U2 18 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD MAY 4 PY 2014 VL 24 IS 5 BP 503 EP 517 DI 10.1080/09583157.2013.874008 PG 15 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF4DC UT WOS:000334660600002 ER PT J AU Manrique, V Diaz, R Erazo, L Reddi, N Wheeler, GS Williams, D Overholt, WA AF Manrique, Veronica Diaz, Rodrigo Erazo, Lenin Reddi, Neha Wheeler, Gregory S. Williams, Dean Overholt, William A. TI Comparison of two populations of Pseudophilothrips ichini (Thysanoptera: Phlaeothripidae) as candidates for biological control of the invasive weed Schinus terebinthifolia (Sapindales: Anacardiaceae) SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE biological control of weeds; NAPPFAST; host-plant haplotypes; MaxEnt; Phlaeothripidae; agent impact studies; Anacardiaceae ID BRAZILIAN PEPPERTREE SAPINDALES; UNITED-STATES; CONTROL AGENTS; COLEOPTERA-CHRYSOMELIDAE; THRIPS-PALMI; HOST-RANGE; FLORIDA; HERBIVORY; ESTABLISHMENT; LEPIDOPTERA AB Brazilian peppertree, Schinus terebinthifolia Raddi (Sapindales: Anacardiaceae) (hereafter Schinus), is one of the worst invasive species in Florida and Hawaii. The thrips Pseudophilothrips ichini Hood (Thysanoptera: Phlaeothripidae) is being considered as a potential biological control agent of Schinus. Two populations of this thrips were collected in the weed's native range; one from central-east Brazil (Ouro Preto thrips) and a second from north-east Brazil (Salvador thrips). Temperature requirements, adult fecundity and impact on different plant haplotypes by P. ichini were examined in the laboratory. Complete development of thrips from both populations occurred at temperatures ranging from 20 to 30 degrees C. Two approaches were used to model the predicted distributions of the thrips populations in the USA: the physiological model (NAPPFAST) based on cold tolerance and the ecological niche model based on climatic variables (MaxEnt). The physiological model predicted that both populations of P. ichini may establish in similar areas of the USA, overlapping with the distribution of Schinus. However, the niche model predicted that only the Ouro Preto thrips could establish in the USA. The difference in model predictions suggests an apparent preadaptation of the Salvador thrips to lower temperatures than those experienced at the locations they were collected in Brazil. The Ouro Preto thrips had similar fecundity on two Florida Schinus haplotypes, whereas lower fecundity on haplotype A was found for the Salvador thrips. Based on these results, the Ouro Preto population may be better adapted to the climatic conditions and plant haplotypes found in Florida. Moreover, greenhouse studies indicated that Schinus growth was greatly reduced by thrips feeding, which may result in lower weed reproduction and densities in the field. C1 [Manrique, Veronica; Diaz, Rodrigo; Erazo, Lenin; Reddi, Neha; Overholt, William A.] Univ Florida, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. [Wheeler, Gregory S.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL USA. [Williams, Dean] Texas Christian Univ, Dept Biol, Ft Worth, TX 76129 USA. RP Manrique, V (reprint author), Univ Florida, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. EM vero72@ufl.edu RI Williams, Dean/H-6108-2016 OI Williams, Dean/0000-0002-9001-6019 FU Florida Fish and Wildlife Conservation Commission; South Florida Water Management District; USDA/ARS; Florida Department of Agriculture and Consumer Services FX Funding for this project was provided by the Florida Fish and Wildlife Conservation Commission (to G..S..W.., W..A..O..), South Florida Water Management District (to G..S..W..), USDA/ARS (to G..S..W..), and the Florida Department of Agriculture and Consumer Services (W..A..O..). NR 63 TC 8 Z9 8 U1 0 U2 13 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD MAY 4 PY 2014 VL 24 IS 5 BP 518 EP 535 DI 10.1080/09583157.2013.878310 PG 18 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF4DC UT WOS:000334660600003 ER PT J AU Busman, M Liu, JH Zhong, HJ Bobell, JR Maragos, CM AF Busman, Mark Liu, Jihong Zhong, Hongjian Bobell, John R. Maragos, Chris M. TI Determination of the aflatoxin AFB1 from corn by direct analysis in real time-mass spectrometry (DART-MS) SO FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT LA English DT Article DE aflatoxin; mass spectrometry; mycotoxin ID LIQUID-CHROMATOGRAPHY; AMBIENT CONDITIONS; ELECTROSPRAY-IONIZATION; FLUORESCENCE DETECTION; B-1 AB Direct analysis in real time (DART) ionisation coupled to a high-resolution mass spectrometer (MS) was used for screening of aflatoxins from a variety of surfaces and the rapid quantitative analysis of a common form of aflatoxin, AFB1, extracted from corn. Sample preparation procedure and instrument parameter settings were optimised to obtain sensitive and accurate determination of aflatoxin AFB1. 84:16 acetonitrile water extracts of corn were analysed by DART-MS. The lowest calibration level (LCL) for aflatoxin AFB1 was 4 mu gkg(-1). Quantitative analysis was performed with the use of matrix-matched standards employing the C-13-labelled internal standard for AFB1. DART-MS of spiked corn extracts gave linear response in the range 4-1000 mu gkg(-1). Good recoveries (94-110%) and repeatabilities (RSD=0.7-6.9%) were obtained at spiking levels of 20 and 100 mu gkg(-1) with the use of an isotope dilution technique. Trueness of data obtained for AFB1 in maize by DART-MS was demonstrated by analysis of corn certified reference materials. C1 [Busman, Mark; Bobell, John R.; Maragos, Chris M.] ARS, Bacterial Foodborne Pathogen & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Liu, Jihong; Zhong, Hongjian] Henan Acad Agr Sci, Res Ctr Agr Qual Stand & Testing Tech, Zhengzhou, Henan, Peoples R China. RP Busman, M (reprint author), ARS, Bacterial Foodborne Pathogen & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM Mark.Busman@ars.usda.gov FU Ministry of Agriculture of the People's Republic of China [2011-G5(3)]; Science and Technology Department of Henan Province [112101110400] FX The authors would like to thank the Ministry of Agriculture of the People's Republic of China [grant number 2011-G5(3)] and Science and Technology Department of Henan Province [grant number 112101110400] for supporting this work. NR 25 TC 9 Z9 9 U1 0 U2 27 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1944-0049 EI 1944-0057 J9 FOOD ADDIT CONTAM A JI Food Addit. Contam. Part A-Chem. PD MAY 4 PY 2014 VL 31 IS 5 BP 932 EP 939 DI 10.1080/19440049.2014.900572 PG 8 WC Chemistry, Applied; Food Science & Technology; Toxicology SC Chemistry; Food Science & Technology; Toxicology GA AH1AK UT WOS:000335851700019 PM 24588621 ER PT J AU Meredith, C Roper, B Archer, E AF Meredith, Christy Roper, Brett Archer, Eric TI Reductions in Instream Wood in Streams near Roads in the Interior Columbia River Basin SO NORTH AMERICAN JOURNAL OF FISHERIES MANAGEMENT LA English DT Article ID SALMON ONCORHYNCHUS-KISUTCH; PACIFIC-NORTHWEST; HEADWATER STREAMS; BULL TROUT; WASHINGTON STREAMS; WESTERN WASHINGTON; HYDRAULIC GEOMETRY; HABITAT CONDITIONS; FEDERAL LANDS; OLD-GROWTH AB Despite the success of recent management efforts to reduce streamside logging, instream wood recovery may be limited by the presence of near-stream roads. We investigated the relationships between the presence of near-stream roads and the frequency and volume of different size-classes of wood in streams in the interior Columbia River basin. We developed models to evaluate the average reduction in instream wood for streams near roads (<30m or 30-60m). We compared this with the changes in wood frequency and volume related to changes in environmental conditions such as precipitation, bank-full width, gradient, and forest cover as well as to changes in grazing-related management. In order to extrapolate our findings to the entire study area, we used a GIS approach to determine the distance to roads for randomly selected sites throughout the study area. Sites <30m from a road had 65 (26%) fewer pieces of total wood, 33 (34%) fewer pieces of coarse wood, 31 (37%) fewer pieces of pool-forming wood, and 37m(3) (42%) less wood volume per kilometer than sites >60m from a road. We also observed significant reductions at sites 30-60m from a road, but these were about half those documented for sites <30m. Changes in environmental conditions and grazing intensity had effects similar to those of being near a road. Based on our GIS analysis, approximately 29% of the sites in the study area are within 60m of a road, and this percentage is even greater if unroaded catchments are excluded. Our results provide strong evidence that the presence of roads has significantly reduced habitat conditions for salmonids in the interior Columbia River basin and illustrate the need for road removal or relocation projects to increase wood in streams. Received April 3, 2013; accepted December 12, 2013 C1 [Meredith, Christy; Roper, Brett; Archer, Eric] US Forest Serv, Logan, UT 84321 USA. RP Meredith, C (reprint author), US Forest Serv, 860 North 1200 East, Logan, UT 84321 USA. EM csmeredith@fs.fed.us FU U.S. Forest Service; Oregon-Washington office of the Bureau of Land Management; Idaho office of the Bureau of Land Management FX We thank the summer field technicians who collected the data for these analyses and Ryan Lokteff for providing the map for this manuscript. We also thank Jeff Ojala, Nate Hough-Snee, and Brian Laub for providing comments on previous versions. Regions 1, 4, and 6 of the U.S. Forest Service and the Oregon-Washington and Idaho offices of the Bureau of Land Management provided funding for this project. NR 73 TC 2 Z9 2 U1 1 U2 15 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0275-5947 EI 1548-8675 J9 N AM J FISH MANAGE JI North Am. J. Fish Manage. PD MAY 4 PY 2014 VL 34 IS 3 BP 493 EP 506 DI 10.1080/02755947.2014.882451 PG 14 WC Fisheries SC Fisheries GA AH1DN UT WOS:000335860100002 ER PT J AU Wisniewski, M Nassuth, A Teulieres, C Marque, C Rowland, J Cao, PB Brown, A AF Wisniewski, Michael Nassuth, Annette Teulieres, Chantal Marque, Christiane Rowland, Jeannine Cao, Phi Bang Brown, Allan TI Genomics of Cold Hardiness in Woody Plants SO CRITICAL REVIEWS IN PLANT SCIENCES LA English DT Review DE freezing tolerance; CBF; COR genes; cold-induced signaling; cold acclimation; Inducer-of-CBF-Expression (ICE) ID PEACH PRUNUS-PERSICA; RESPONSIVE GENE-EXPRESSION; CBF TRANSCRIPTIONAL ACTIVATORS; RELATIVE PONCIRUS-TRIFOLIATA; ENHANCES FREEZING TOLERANCE; ABIOTIC STRESS RESPONSES; QUANTITATIVE TRAIT LOCI; HIGH-SALINITY STRESS; X DOMESTICA BORKH.; 2 DEHYDRIN GENES AB The term cold hardiness or freezing tolerance is used to represent, in a general sense, the ability of plants to adapt to and withstand freezing temperatures. It is a complex, multigenic trait that is too often viewed as a single entity when in fact it is composed of many aspects, all of which can be to some extent viewed as genetically distinct. Advances in molecular biology and genomics have provided significant advances in understanding how plants respond to low temperature and acquire freezing tolerance. Among the most important discoveries has been the identification of the CBF/DREB transcription factor. This transcription factor, along with its regulators such as ICE transcription factors, play a major role in sensing low temperature, initiating the process of cold acclimation, and inducing the expression of a large set of cold-regulated genes. These latter genes are presumed to ameliorate injury to plant cells as a result of freeze-induced desiccation and the presence of extracellular ice. The present review provides a comprehensive overview of CBF and ICE genes in a number of woody plants whose genomes have been sequenced and provides information on the attempts to identify genetic markers for use in marker-assisted selection (MAS) or to improve cold hardiness using genetic transformation technologies. Functional studies of CBF genes in woody plants have indicated that their regulation and impact on abiotic stress resistance are more complex than in herbaceous plants. In particular, the possible relationship of CBF to dormancy is highlighted. Cold hardiness is a complex trait and the challenge in the future will be to use the molecular and genetic tools that are being developed, as well as new developments in bioinformatics, to integrate complex sets of data into a systems view of plant biology. This approach holds the best promise for developing the ability to significantly improve cold hardiness in economically important crops while still maintaining high levels of plant productivity and yield. C1 [Wisniewski, Michael] ARS, USDA, Kearneysville, WV USA. [Nassuth, Annette] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Teulieres, Chantal; Marque, Christiane; Cao, Phi Bang] Univ Toulouse, LRSV Lab Res Plant Sci, Toulouse, France. [Rowland, Jeannine] ARS, USDA, Beltsville, MD USA. [Brown, Allan] N Carolina State Univ, Kannapolis, NC USA. RP Wisniewski, M (reprint author), USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.wisniewski@ars.usda.gov RI CAO, Phi Bang/A-7688-2016 FU NSERC; ORF FX The research on the grape CBF pathway in the Nassuth lab is supported by grants from NSERC and ORF. The work on Eucalyptus CBF by the French team is carried out in the framework of the LabEx TULIP project (Towards a Unified theory of biotic Interactions: the roLe of environmental Perturbations). French embassies in Canada and the United States also contributed to this review by supporting the collaboration between the co-authors. This review covers research reported in the literature through January 2013. NR 240 TC 12 Z9 12 U1 6 U2 102 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0735-2689 EI 1549-7836 J9 CRIT REV PLANT SCI JI Crit. Rev. Plant Sci. PD MAY 4 PY 2014 VL 33 IS 2-3 SI SI BP 92 EP 124 DI 10.1080/07352689.2014.870408 PG 33 WC Plant Sciences SC Plant Sciences GA AE4DJ UT WOS:000333930500003 ER PT J AU Dukowic-Schulze, S Sundararajan, A Mudge, J Ramaraj, T Farmer, AD Wang, MH Sun, Q Pillardy, J Kianian, S Retzel, EF Pawlowski, WP Chen, CB AF Dukowic-Schulze, Stefanie Sundararajan, Anitha Mudge, Joann Ramaraj, Thiruvarangan Farmer, Andrew D. Wang, Minghui Sun, Qi Pillardy, Jaroslaw Kianian, Shahryar Retzel, Ernest F. Pawlowski, Wojciech P. Chen, Changbin TI The transcriptome landscape of early maize meiosis SO BMC PLANT BIOLOGY LA English DT Article DE Maize; Meiosis; Meiocytes; Mitochondria; RNA-seq; Transcriptome ID MEIOTIC RECOMBINATION HOTSPOT; ARABIDOPSIS MALE MEIOCYTES; CYTOPLASMIC MALE-STERILITY; GENE-EXPRESSION; MITOCHONDRIAL GENE; ANTHER DEVELOPMENT; DIFFERENTIAL EXPRESSION; PROPHASE-I; PLANTS; RICE AB Background: A major step in the higher plant life cycle is the decision to leave the mitotic cell cycle and begin the progression through the meiotic cell cycle that leads to the formation of gametes. The molecular mechanisms that regulate this transition and early meiosis remain largely unknown. To gain insight into gene expression features during the initiation of meiotic recombination, we profiled early prophase I meiocytes from maize (Zea mays) using capillary collection to isolate meiocytes, followed by RNA-seq. Results: We detected similar to 2,000 genes as preferentially expressed during early meiotic prophase, most of them uncharacterized. Functional analysis uncovered the importance of several cellular processes in early meiosis. Processes significantly enriched in isolated meiocytes included proteolysis, protein targeting, chromatin modification and the regulation of redox homeostasis. The most significantly up-regulated processes in meiocytes were processes involved in carbohydrate metabolism. Consistent with this, many mitochondrial genes were up-regulated in meiocytes, including nuclear-and mitochondrial-encoded genes. The data were validated with real-time PCR and in situ hybridization and also used to generate a candidate maize homologue list of known meiotic genes from Arabidopsis. Conclusions: Taken together, we present a high-resolution analysis of the transcriptome landscape in early meiosis of an important crop plant, providing support for choosing genes for detailed characterization of recombination initiation and regulation of early meiosis. Our data also reveal an important connection between meiotic processes and altered/ increased energy production. C1 [Dukowic-Schulze, Stefanie; Chen, Changbin] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Sundararajan, Anitha; Mudge, Joann; Ramaraj, Thiruvarangan; Farmer, Andrew D.; Retzel, Ernest F.] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Wang, Minghui; Pawlowski, Wojciech P.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Wang, Minghui; Sun, Qi; Pillardy, Jaroslaw] Cornell Univ, Computat Biol Serv Unit, Ithaca, NY 14850 USA. [Kianian, Shahryar] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Chen, CB (reprint author), Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. EM chenx481@umn.edu RI Wang, Minghui/G-4939-2015; OI Dukowic-Schulze, Stefanie/0000-0003-2675-1075 FU National Science Foundation [IOS: 1025881] FX We thank A. Harris, J. Jensen, R. Meissner for plant care. J. Cohen, A. Hegeman for discussions and technique support. This work is supported by the National Science Foundation (IOS: 1025881) to W.P., S.K., J.P., J.M., E.R. and C.C. NR 79 TC 12 Z9 13 U1 2 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD MAY 3 PY 2014 VL 14 AR 118 DI 10.1186/1471-2229-14-118 PG 14 WC Plant Sciences SC Plant Sciences GA AI4UP UT WOS:000336860600001 PM 24885405 ER PT J AU Pontius, J AF Pontius, Jennifer TI A new approach for forest decline assessments: maximizing detail and accuracy with multispectral imagery SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID LEAF-AREA INDEX; HYPERSPECTRAL VEGETATION INDEXES; THEMATIC MAPPER DATA; CHLOROPHYLL FLUORESCENCE; SPECTRAL REFLECTANCE; PLANT STRESS; OPTICAL-PROPERTIES; ALGORITHM DEVELOPMENT; WATER-STRESS; MAPLE LEAVES AB Remote sensing of forest condition is typically based on broadband vegetation indices to quantify coarse categories of canopy condition. More detailed and accurate assessments have been demonstrated using narrowband sensors, although with more limited image availability. While differences in sensor capabilities are obvious, I hypothesized that multispectral imagery may be able to detect more subtle canopy stress symptoms if a new calibration approach was considered. This involves three major changes to traditional decline assessments: (1) calibration with more detailed field measurements, (2) consideration of narrowband derived indices adapted for broadband calculation, and (3) a multivariate calibration model. Testing this approach on Landsat-5 (TM) imagery in the Catskills, NY, USA, a five-term linear regression model (r(2)=0.621, RMSE 0.403) based on a unique combination of vegetation indices sensitive to canopy chlorophyll, carotenoids, green leaf area, and water content was able to quantify a broad range of forest condition across species. When rounded to a class-based system for comparison to more traditional methods, this equation predicted decline across 42 mixed-species plots with 65% accuracy (10-classes), and 100% accuracy (5-classes). This approach was a significant improvement over commonly used vegetation indices such as NDVI (r(2)=0.351, RMSE=0.500, 10-class accuracy=60%, and 5-class accuracy=74%). These results suggest that relying solely on a single common vegetation index to assess forest condition may artificially limit the accuracy and detail possible with multispectral imagery. I recommend that future efforts to monitor forest decline consider this three-pronged approach to decline predictions in order to maximize the information and accuracy obtainable with broadband sensors so widely available at this time. C1 [Pontius, Jennifer] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Pontius, Jennifer] US Forest Serv, USDA, No Res Stn, Burlington, VT 05405 USA. RP Pontius, J (reprint author), Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. EM Jennifer.pontius@uvm.edu NR 81 TC 1 Z9 1 U1 1 U2 20 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD MAY 3 PY 2014 VL 35 IS 9 BP 3384 EP 3402 DI 10.1080/01431161.2014.903439 PG 19 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA AF7OU UT WOS:000334905200025 ER PT J AU Lu, Y Zhao, HY Sun, J Liu, YQ Zhou, XP Beier, RC Wu, GJ Hou, XL AF Lu, Yan Zhao, Hongyu Sun, Jian Liu, Yuqi Zhou, Xuping Beier, Ross C. Wu, Guojuan Hou, Xiaolin TI Characterization of Multidrug-Resistant Salmonella enterica Serovars Indiana and Enteritidis from Chickens in Eastern China SO PLOS ONE LA English DT Article ID GENE CASSETTES; CLASS-1 INTEGRONS; SLAUGHTER; DIVERSITY; BACTERIA; ANIMALS AB A total of 310 Salmonella isolates were isolated from 6 broiler farms in Eastern China, serotyped according to the Kauffmann-White classification. All isolates were examined for susceptibility to 17 commonly used antimicrobial agents, representative isolates were examined for resistance genes and class I integrons using PCR technology. Clonality was determined by pulsed-field gel electrophoresis (PFGE). There were two serotypes detected in the 310 Salmonella strains, which included 133 Salmonella enterica serovar Indiana isolates and 177 Salmonella enterica serovar Enteritidis isolates. Antimicrobial sensitivity results showed that the isolates were generally resistant to sulfamethoxazole, ampicillin, tetracycline, doxycycline and trimethoprim, and 95% of the isolates sensitive to amikacin and polymyxin. Among all Salmonella enterica serovar Indiana isolates, 108 (81.2%) possessed the bla(TEM), floR, tetA, strA and aac (6')-Ib-cr resistance genes. The detected carriage rate of class 1 integrons was 66.5% (206/310), with 6 strains carrying gene integron cassette dfr17-aadA5. The increasing frequency of multidrug resistance rate in Salmonella was associated with increasing prevalence of int1 genes (r(s) = 0.938, P = 0.00039). The int1, bla(TEM), floR, tetA, strA and aac (6')-Ib-cr positive Salmonella enterica serovar Indiana isolates showed five major patterns as determined by PFGE. Most isolates exhibited the common PFGE patterns found from the chicken farms, suggesting that many multidrug-resistant isolates of Salmonella enterica serovar Indiana prevailed in these sources. Some isolates with similar antimicrobial resistance patterns represented a variety of Salmonella enterica serovar Indiana genotypes, and were derived from a different clone. C1 [Lu, Yan; Liu, Yuqi; Zhou, Xuping; Wu, Guojuan; Hou, Xiaolin] China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China. [Zhao, Hongyu] Natl Inst Biol Sci, Beijing, Peoples R China. [Sun, Jian] Beijing Vocat Coll Agr, Dept Anim Husb & Vet Med, Beijing, Peoples R China. [Beier, Ross C.] USDA ARS, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX USA. RP Hou, XL (reprint author), China Agr Univ, Coll Anim Sci & Technol, Beijing 100094, Peoples R China. EM hxlsx@163.com FU National Natural Science Foundation of China [31201949, 31172362]; Scientific Research Improvement Foundation of Beijing University of Agriculture [GJB2012003] FX This study was supported by the National Natural Science Foundation of China (31201949), the National Natural Science Foundation of China (31172362); it was also supported by the Scientific Research Improvement Foundation of Beijing University of Agriculture (GJB2012003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 27 TC 4 Z9 5 U1 1 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 2 PY 2014 VL 9 IS 5 AR e96050 DI 10.1371/journal.pone.0096050 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI1ZS UT WOS:000336655700053 PM 24788434 ER PT J AU Gyore, J Parameswar, AR Hebbard, CFF Oh, Y Bi, EF Demchenko, AV Price, NP Orlean, P AF Gyore, Jacob Parameswar, Archana R. Hebbard, Carleigh F. F. Oh, Younghoon Bi, Erfei Demchenko, Alexei V. Price, Neil P. Orlean, Peter TI 2-Acylamido Analogues of N-Acetylglucosamine Prime Formation of Chitin Oligosaccharides by Yeast Chitin Synthase 2 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article DE Carbohydrate Biosynthesis; Chitin; Glycosyltransferases; Polysaccharide; Yeast Physiology ID SACCHAROMYCES-CEREVISIAE; CELLULOSE SYNTHESIS; CELL-WALL; PURIFICATION; BIOSYNTHESIS; SYNTHETASE; EXTRACTION; BINDING; SITES; FUNGI AB Background: Chitin synthases are stimulated by N-acetylglucosamine (GlcNAc). Results: GlcNAc and 2-acylamido analogues of GlcNAc stimulate formation of chitin oligosaccharides by yeast chitin synthase, and GlcNAc is transferred to the 2-acylamido analogues. Conclusion: Chitin synthases use GlcNAc analogues as primers and transfer one GlcNAc at a time. Significance: Results are new insights into polysaccharide synthase mechanism and suggest ways of synthesizing novel modified polysaccharides. Chitin, a homopolymer of 1,4-linked N-acetylglucosamine (GlcNAc) residues, is a key component of the cell walls of fungi and the exoskeletons of arthropods. Chitin synthases transfer GlcNAc from UDP-GlcNAc to preexisting chitin chains in reactions that are typically stimulated by free GlcNAc. The effect of GlcNAc was probed by using a yeast strain expressing a single chitin synthase, Chs2, by examining formation of chitin oligosaccharides (COs) and insoluble chitin, and by replacing GlcNAc with 2-acylamido analogues of GlcNAc. Synthesis of COs was strongly dependent on inclusion of GlcNAc in chitin synthase incubations, and N,N-diacetylchitobiose (GlcNAc(2)) was the major reaction product. Formation of both COs and insoluble chitin was also stimulated by GlcNAc(2) and by N-propanoyl-, N-butanoyl-, and N-glycolylglucosamine. MALDI analyses of the COs made in the presence of 2-acylamido analogues of GlcNAc showed they that contained a single GlcNAc analogue and one or more additional GlcNAc residues. These results indicate that Chs2 can use certain 2-acylamido analogues of GlcNAc, and likely free GlcNAc and GlcNAc(2) as well, as GlcNAc acceptors in a UDP-GlcNAc-dependent glycosyltransfer reaction. Further, formation of modified disaccharides indicates that CSs can transfer single GlcNAc residues. C1 [Gyore, Jacob; Hebbard, Carleigh F. F.; Orlean, Peter] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA. [Parameswar, Archana R.; Demchenko, Alexei V.] Univ Missouri, Dept Chem & Biochem, St Louis, MO 63121 USA. [Oh, Younghoon; Bi, Erfei] Univ Penn, Perelman Sch Med, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA. [Price, Neil P.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Orlean, P (reprint author), Univ Illinois, Dept Microbiol, 601 S Goodwin Ave, Urbana, IL 61801 USA. EM p-orlean@illinois.edu OI Demchenko, Alexei/0000-0003-3515-212X FU National Institutes of Health [GM077170, GM87365] FX This work was supported, in whole or in part, by National Institutes of Health Grants GM077170 (to A.V.D.) and GM87365 (to E.B.). NR 33 TC 1 Z9 1 U1 1 U2 19 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD MAY 2 PY 2014 VL 289 IS 18 BP 12835 EP 12841 DI 10.1074/jbc.M114.550749 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AG7ER UT WOS:000335581400052 PM 24619411 ER PT J AU Ort, DR Long, SP AF Ort, Donald R. Long, Stephen P. TI Limits on Yields in the Corn Belt SO SCIENCE LA English DT Editorial Material C1 [Ort, Donald R.] Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Ort, Donald R.; Long, Stephen P.] Univ Illinois, Dept Plant Biol & Crop Sci, Urbana, IL 61801 USA. RP Ort, DR (reprint author), Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM d-ort@igb.illinois.edu RI Long, Stephen/A-2488-2008 OI Long, Stephen/0000-0002-8501-7164 NR 8 TC 29 Z9 29 U1 5 U2 52 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD MAY 2 PY 2014 VL 344 IS 6183 BP 483 EP 484 DI 10.1126/science.1253884 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG1EK UT WOS:000335157700032 PM 24786071 ER PT J AU Sanderson, MA Stout, R Goslee, S Gonet, J Smith, RG AF Sanderson, Matt A. Stout, Robert Goslee, Sarah Gonet, Jeff Smith, Richard G. TI Soil seed bank community structure of pastures and hayfields on an organic farm SO CANADIAN JOURNAL OF PLANT SCIENCE LA English DT Article DE Alfalfa; botanical composition; forage; weed management ID NORTHEAST UNITED-STATES; DISTURBANCE; VEGETATION; GRASSLAND; INTENSITY; DIVERSITY; SHIFTS; LANDS AB Understanding the composition of seed banks in pasture soils would help farmers anticipate and manage for weed problems. We characterized the soil seed bank in eight pastures and hayfields [two alfalfa (Medicago sativa L.) and two predominantly grass hayfields; two recently established and two permanent pastures] within an organic dairy farm in southeastern New Hampshire. Seed banks were sampled in the upper 5 cm of soil in each field at a point scale in 2007 and 2010. In 2010, the seed bank was characterized at the field scale by taking soil samples on six 52-m transects in each field. Seed banks sampled at the field scale in 2010 contained 66 plant species. The total number of seeds in the seed bank ranged from 1560 m(-2) in grass hayfields in autumn to more than 20 000 m(-2) in alfalfa hayfields in summer. Annual forbs dominated the seed bank of alfalfa fields and recently established pastures, whereas perennial graminoids dominated in one grass hayfield and the permanent pastures. These results suggest that management history affects soil seed bank composition and abundance, and these effects should be considered before implementing management practices that could stimulate recruitment from the seed bank. C1 [Sanderson, Matt A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Stout, Robert; Goslee, Sarah; Gonet, Jeff] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Smith, Richard G.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. RP Sanderson, MA (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM matt.sanderson@ars.usda.gov NR 20 TC 1 Z9 2 U1 0 U2 9 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-4220 EI 1918-1833 J9 CAN J PLANT SCI JI Can. J. Plant Sci. PD MAY PY 2014 VL 94 IS 4 BP 621 EP 631 DI 10.4141/CJPS2013-288 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA CA8EX UT WOS:000349150900003 ER PT J AU Gipson, C Brown, P AF Gipson, Chester Brown, Patricia TI A word from USDA and OLAW SO LAB ANIMAL LA English DT Editorial Material C1 [Gipson, Chester] USDA, APHIS, AC, Washington, DC 20250 USA. [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD USA. RP Gipson, C (reprint author), USDA, APHIS, AC, Washington, DC 20250 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD MAY PY 2014 VL 43 IS 5 BP 159 EP 159 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA AZ2ZC UT WOS:000348096700014 PM 24751846 ER PT J AU Almeyda, CV Neal, LC Abernethy, T Abad, JA Pesic-Vanesbroeck, Z AF Almeyda, C. V. Neal, L. C. Abernethy, T. Abad, J. A. Pesic-Vanesbroeck, Z. TI From research stations to commercial fields: Survey, detection and identification of sweetpotato viruses in North Carolina SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Almeyda, C. V.; Neal, L. C.; Abernethy, T.; Pesic-Vanesbroeck, Z.] N Carolina State Univ, Raleigh, NC 27695 USA. [Abad, J. A.] APHIS, USDA, PPQ FO PGQP, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 1 EP 2 PG 2 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500005 ER PT J AU Brenneman, T Tsai, YC Webster, T Scully, B Davis, R Grey, T AF Brenneman, T. Tsai, Y. C. Webster, T. Scully, B. Davis, R. Grey, T. TI Fungicide timing for management of sugar beet diseases in Georgia SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Brenneman, T.; Tsai, Y. C.; Grey, T.] UGA, Tifton, GA USA. [Webster, T.; Scully, B.; Davis, R.] USDA, Tifton, GA USA. RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 2 EP 2 PG 1 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500007 ER PT J AU Ferguson, MH Clark, CA Smith, BJ AF Ferguson, M. H. Clark, C. A. Smith, B. J. TI Xylella fastidiosa infection is correlated with lower yield in a rabbiteye blueberry orchard in Louisiana SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Ferguson, M. H.; Clark, C. A.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Smith, B. J.] ARS, USDA, Southern Hort Lab, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 4 EP 4 PG 1 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500017 ER PT J AU Garrido, PA Proano, F Diaz, EA Daughtrey, M Levesque, A Moorman, GW Herrero, M Klemsdal, SS Weiland, J Garzon, CD AF Garrido, P. A. Proano, F. Diaz, E. A. Daughtrey, M. Levesque, A. Moorman, G. W. Herrero, M. Klemsdal, S. S. Weiland, J. Garzon, C. D. TI Evolutionary relationship among species of the Pythium irregulare complex SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Garrido, P. A.; Proano, F.; Diaz, E. A.; Garzon, C. D.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Daughtrey, M.] Cornell Univ, Riverhead, NY USA. [Levesque, A.] Agr & Agri Food Canada, Toronto, ON, Canada. [Moorman, G. W.] Penn State Univ, State Coll, PA USA. [Herrero, M.; Klemsdal, S. S.] Bioforsk, Hogskoleveien, Norway. [Weiland, J.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 4 EP 5 PG 2 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500021 ER PT J AU Luis, JMS Ozias-Akins, P Chu, Y Glenn, AE Holbrook, CC Kemerait, RC AF Luis, J. M. S. Ozias-Akins, P. Chu, Y. Glenn, A. E. Holbrook, C. C. Kemerait, R. C. TI Detection and quantification of aflatoxigenic fungi in drought-stressed peanut seeds through quantitative real-time PCR SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Luis, J. M. S.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Ozias-Akins, P.; Chu, Y.] Univ Georgia, Dept Hort, Tifton, GA USA. [Ozias-Akins, P.; Chu, Y.] Univ Georgia, NESPAL, Tifton, GA USA. [Glenn, A. E.] USDA ARS, Toxicol & Mycotoxin Res Unit, Athens, GA 30613 USA. [Holbrook, C. C.] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Kemerait, R. C.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 7 EP 7 PG 1 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500035 ER PT J AU Luis, JS Luis, JMS Glenn, AE Bacon, CW Kemerait, RC AF Luis, J. S. Luis, J. M. S. Glenn, A. E. Bacon, C. W. Kemerait, R. C. TI Species and strain differentiation of Aspergillus section Flavi through morphological characterization and repetitive-sequence-based PCR DNA fingerprinting SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Luis, J. S.] Benguet State Univ, La Trinidad, Benguet, Philippines. [Luis, J. M. S.; Kemerait, R. C.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Glenn, A. E.; Bacon, C. W.] USDA ARS, Toxicol & Mycotoxin Res Unit, Athens, GA 30613 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 7 EP 7 PG 1 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500034 ER PT J AU Singh, R Hardy, TN Spitzer, WE AF Singh, R. Hardy, T. N. Spitzer, W. E. TI Citrus canker: Another hurricane for Louisiana's citrus industry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Meeting of the Southern-Division of American-Phytopathological-Society (APS) CY FEB 02-03, 2014 CL Dallas, TX SP Amer Phytopathol Soc, So Div C1 [Singh, R.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Hardy, T. N.] Louisiana Dept Agr & Forestry, Baton Rouge, LA USA. [Spitzer, W. E.] USDA APHIS PPQ, Baton Rouge, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 SU 2 BP 9 EP 9 PG 1 WC Plant Sciences SC Plant Sciences GA AW5FY UT WOS:000346302500045 ER PT J AU Klindworth, DL Hareland, GA Elias, EM Ohm, JB Puhr, D Xu, SS AF Klindworth, Daryl L. Hareland, Gary A. Elias, Elias M. Ohm, Jae-Bom Puhr, Dehdra Xu, Steven S. TI Interactions of Genotype and Glutenin Subunit Composition on Breadmaking Quality of Durum 1AS.1AL-1DL Translocation Lines SO CEREAL CHEMISTRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; WHEAT TRITICUM-AESTIVUM; SIZE DISTRIBUTION; TRANSLOCATION LINES; PROTEIN-COMPOSITION; MIXING PROPERTIES; BAKING QUALITY; DOUGH STRENGTH; FLOUR PROTEINS; LOAF VOLUME AB Dual-purpose durum (Triticum turgidum L. subsp. durum) wheat, having both good pasta and breadmaking quality, would be an advantage in the market. In this study, we evaluated the effects of genotype and varying BMW and LMW glutenin subunit composition on durum breadmaking quality. Genotypes included five near-isogenic backgrounds that also differed by variability at the Glu-Did (BMW subunits 1Dx5+1Dy10), Glu-BI (presence or absence of subunit 1By8), and Glu-B3 (LMWI or LMWII pattern) loci. Quality tests were conducted on genotypes grown at five North Dakota locations. Genotype had a stronger influence on free asparagine content than glutenin subunit composition. Genotypes carry- ing Glu-Did had higher glutenin content than lines that did not carry GluDid. Among Rugby translocation genotypes, lines carrying LMWI had higher gliadin content and better loaf volume than genotypes carrying LMWII. Absence of 1By8 produced major reductions in loaf volume in nontranslocation lines regardless of whether LMWI or LMWII was present. In contrast, the presence of Glu-Did compensated well for the absence of 1By8 regardless of which LMW pattern was present. The durum genotypes did not have loaf volumes equal to bread wheat cultivars, and results suggest that improved extensibility is needed to improve durum breadmaking quality. C1 [Klindworth, Daryl L.; Hareland, Gary A.; Ohm, Jae-Bom; Puhr, Dehdra; Xu, Steven S.] ARS, USDA, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. [Elias, Elias M.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Klindworth, DL (reprint author), ARS, USDA, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. EM Daryl.Klindworth@ars.usda.gov FU U.S. Department of Agriculture (USDA) CRIS Project [5442-22000-033-00D] FX The authors thank Frank Manthey and Xiwen Cai for critically reviewing the manuscript and Stan Stancyk, Jadene Wear, and Sherry Jiang for technical support. This research was supported by the U.S. Department of Agriculture (USDA) CRIS Project No. 5442-22000-033-00D. NR 53 TC 0 Z9 0 U1 0 U2 9 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2014 VL 91 IS 3 BP 211 EP 217 DI 10.1094/CCHEM-08-13-0165-R PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AR7SH UT WOS:000343779400002 ER PT J AU Cremer, JE Liu, LM Bean, SR Ohm, JB Tilley, M Wilson, JD Kaufman, RC Vu, TH Gilding, EK Godwin, ID Wang, DH AF Cremer, Julia E. Liu, Liman Bean, Scott R. Ohm, Jae-Bom Tilley, Michael Wilson, Jeff D. Kaufman, Rhett C. Vu, Thanh H. Gilding, Edward K. Godwin, Ian D. Wang, Donghai TI Impacts of Kafirin Allelic Diversity, Starch Content, and Protein Digestibility on Ethanol Conversion Efficiency in Grain Sorghum SO CEREAL CHEMISTRY LA English DT Article ID BICOLOR L. MOENCH; SEED WEIGHT; ENDOSPERM TEXTURE; GENETIC-ANALYSIS; FEED QUALITY; MAIZE; FERMENTATION; HYBRIDS; MUTANT; ZEIN AB Seed protein and starch composition determine the efficiency of the fermentation process in the production of grain-based ethanol. Sorghum, a highly water- and nutrient-efficient plant, provides an alternative to fuel crops with greater irrigation and fertilizer requirements, such as maize. However, sorghum grain is generally less digestible because of extensive disulfide cross-linking among sulfur-rich storage proteins in the protein starch matrix. Thus, the fine structure and composition of the seed endosperm directly impact grain end use, including fermentation performance. To test the hypothesis that kafirin (prolamin) seed storage proteins specifically influence the efficiency of ethanol production from sorghum, 10 diverse genetic lines with allelic variation in the beta-, gamma-, and delta-kafirins, including three P-kafirin null mutants, were tested for ethanol yield and fermentation efficiency. Our selected lines showed wide variation in grain biochemical features, including total protein (9.96-16.47%), starch (65.52-74.29%), and free amino nitrogen (FAN) (32.84-73.51 mg/L). Total ethanol yield (ranging from 384 to 426 L/metric ton), was positively correlated to starch content (R-2 = 0.74), and there was a slight positive correlation between protein digestibility and ethanol yield (R-2 = 0.52). Increases in FAN content enhanced fermentation efficiency (R-2 = 0.65). The highest ethanol producer was elite stay green breeding line B923296, and the line with the highest fermentation efficiency at the 72 h time point was inbred BTx623. A large-seeded genotype, KS115, carrying a novel gamma-kafirin allele, was rich in FAN and exhibited excellent short-term fermentation efficiency at 85.68% at the 20 h time point. However, the overall ethanol yield from this line was comparatively low at 384 L/metric ton, because of insufficient starch, low digestibility, and high crude protein. Multivariate analysis indicated an association between the beta-kafirin allele and variation in grain digestibility (P = 0.042) and FAN (P = 0.036), with subsequent effects on ethanol yield. Reversed-phase HPLC profiling of the alcohol-soluble kafirin protein fraction revealed diversity in protein content and composition across the lines, with similarities in peak distribution profiles among beta-kafirin null mutants compared with normal lines. C1 [Cremer, Julia E.; Godwin, Ian D.] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia. [Liu, Liman; Wang, Donghai] Kansas State Univ, Dept Biol & Agr Engn, Manhattan, KS 66506 USA. [Bean, Scott R.; Tilley, Michael; Wilson, Jeff D.; Kaufman, Rhett C.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Ohm, Jae-Bom] ARS, USDA, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Vu, Thanh H.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Gilding, Edward K.] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia. RP Cremer, JE (reprint author), Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia. EM j.cremer@uq.edu.au RI Gilding, Edward/A-8630-2011; OI Gilding, Edward/0000-0003-1720-1282; Cremer, Julia Erin/0000-0001-8488-7451; Bean, Scott/0000-0001-8678-8094 FU Graduate School International Travel Award (GSITA) by the University of Queensland; Australian Postgraduate Award Industry Scholarship; Australian Research Council; Pacific Seeds [LP0883808]; Andersons Research Grant [NC-213]; Kansas State University, Manhattan, Kansas FX Julia E. Cremer was supported by a Graduate School International Travel Award (GSITA) provided by the University of Queensland and an Australian Postgraduate Award Industry Scholarship, as supported by the Australian Research Council and Pacific Seeds (linkage project LP0883808). Research conducted at the USDA-ARS in Manhattan, Kansas, was partially supported by an NC-213 Andersons Research Grant. Additional research funding was provided by Kansas State University, Manhattan, Kansas. Assistance with statistical analysis was kindly provided by Delma Greenway and Margaret Jewell at the University of Queensland. NR 56 TC 2 Z9 2 U1 2 U2 17 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2014 VL 91 IS 3 BP 218 EP 227 DI 10.1094/CCHEM-04-13-0068-R PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AR7SH UT WOS:000343779400003 ER PT J AU Choi, HW Baik, BK AF Choi, Hyun-Wook Baik, Byung-Kee TI Significance of Starch Properties and Quantity on Sponge Cake Volume SO CEREAL CHEMISTRY LA English DT Article ID SOFT WHEATS; QUALITY CHARACTERISTICS; GROWING ENVIRONMENT; PASTING PROPERTIES; AMYLOSE CONTENT; BAKING; FLOUR; WAXY; HARDNESS; NOODLES AB We evaluated the qualitative and quantitative effects of wheat starch on sponge cake (SC) baking quality. Twenty wheat flours, including soft white and club wheat of normal, partial waxy, and waxy endosperm, as well as hard wheat, were tested for amylose content, pasting properties, and SC baking quality. Starches isolated from wheat flours of normal, single-null partial waxy, double-null partial waxy, and waxy endosperm were also tested for pasting properties and baked into SC. Double-null partial waxy and waxy wheat flours produced SC with volume of 828-895 mL, whereas volume of SC baked from normal and single-null partial waxy wheat flours ranged from 1,093 to 1,335 mL. The amylose content of soft white and club wheat flour was positively related to the volume of SC (r = 0.790, P < 0.001). Pasting temperature, peak viscosity, final viscosity, breakdown, and setback also showed significant relationships with SC volume. Normal and waxy starch blends having amylose contents of 25, 20, 15, and 10% produced SCs with volume of 1,570, 1,435, 1,385, and 1,185 mL, respectively. At least 70 g of starch or at least 75% starch in 100 g of starch gluten blend in replacement of 100 g of wheat flour in the SC baking formula was needed to produce SC having the maximum volume potential. Starch properties including amylose content and pasting properties as well as proportion of starch evidently play significant roles in SC baking quality of wheat flour. C1 [Choi, Hyun-Wook] Korea Food Res Inst, Songnam 463746, South Korea. [Choi, Hyun-Wook] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Baik, Byung-Kee] ARS, USDA, Soft Wheat Qual Lab, Wooster, OH 44691 USA. RP Baik, BK (reprint author), ARS, USDA, Soft Wheat Qual Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM byungkee.baik@ars.usda.gov FU Washington Grain Commission (Spokane, WA, U.S.A.) FX This study was conducted with the financial support of the Washington Grain Commission (Spokane, WA, U.S.A.). NR 18 TC 1 Z9 1 U1 2 U2 12 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2014 VL 91 IS 3 BP 280 EP 285 DI 10.1094/CCHEM-06-13-0114-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AR7SH UT WOS:000343779400012 ER PT J AU Park, JB AF Park, Jae B. TI Synthesis, HPLC Measurement and Bioavailability of the Phenolic Amide Amkamide SO JOURNAL OF CHROMATOGRAPHIC SCIENCE LA English DT Article ID PLASMA AB Amkamide is a phenolic amide whose analogues were recently reported to have potent mitochondria protective activity, although their bioavailability is still unknown. Therefore, in this study, amkamide was synthesized and confirmed by nuclear magnetic resonance, and a high-performance liquid chromatography (HPLC) method was developed for analyzing the amides in biological samples. HPLC separation was performed on a Nova-Pak C18 column using a gradient condition and a coulometric electrochemical detector. The HPLC method was able to produce excellent and reproducible separations of amkamide from other amides (oretamide, becatamide, enferamide and veskamide). All five amides yielded outstanding peak resolutions with detection limits as low as 100 fmol. Therefore, the bioavailability of amkamide together with becatamide was determined in a mouse model. Their plasma concentrations were measured following two oral administrations (2 and 4 mg per 30 g body weight) using the HPLC method. As expected, relatively high amounts of amkamide and becatamide (51-58 mu M) were detected at the time after administration when the maximum concentration was reached (approximately 20 and 25 min) at the high dose. The data indicate that HPLC methods with amide standards may be utilized to measure amkamide and its analogues in biological samples with excellent resolution and detection limits. C1 USDA ARS, BHNRC, Diet Genom & Immunol Lab, Washington, DC 20250 USA. RP Park, JB (reprint author), USDA ARS, BHNRC, Diet Genom & Immunol Lab, Washington, DC 20250 USA. EM jae.park@ars.usda.gov NR 11 TC 0 Z9 0 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0021-9665 EI 1945-239X J9 J CHROMATOGR SCI JI J. Chromatogr. Sci. PD MAY-JUN PY 2014 VL 52 IS 5 BP 436 EP 441 DI 10.1093/chromsci/bmt055 PG 6 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA AQ4AM UT WOS:000342734600011 PM 23712832 ER PT J AU Soltani, A Ghavami, F Mergoum, M Hegstad, J Noyszewski, A Meinhardt, S Kianian, SF AF Soltani, Ali Ghavami, Farhad Mergoum, Mohamed Hegstad, Justin Noyszewski, Andrzej Meinhardt, Steven Kianian, Shahryar F. TI Analysis of ATP6 sequence diversity in the Triticum-Aegilops species group reveals the crucial role of rearrangement in mitochondrial genome evolution SO GENOME LA English DT Article DE ATP6; mitochondria; wheat; rearrangement; duplication ID BREAK-INDUCED REPLICATION; CYTOCHROME-OXIDASE; GENETIC DIVERSITY; CHLOROPLAST DNA; COMMON WHEAT; DURUM-WHEAT; TIMOPHEEVI; DIFFERENTIATION; HETEROPLASMY; CYTOPLASM AB Mutation and chromosomal rearrangements are the two main forces of increasing genetic diversity for natural selection to act upon, and ultimately drive the evolutionary process. Although genome evolution is a function of both forces, simultaneously, the ratio of each can be varied among different genomes and genomic regions. It is believed that in plant mitochondrial genome, rearrangements play a more important role than point mutations, but relatively few studies have directly addressed this phenomenon. To address this issue, we isolated and sequenced the ATP6-1 and ATP6-2 genes from 46 different euplasmic and alloplasmic wheat lines. Four different ATP6-1 orthologs were detected, two of them reported for the first time. Expression analysis revealed that all four orthologs are transcriptionally active. Results also indicated that both point mutation and genomic rearrangement are involved in the evolution of ATP6. However, rearrangement is the predominant force that triggers drastic variation. Data also indicated that speciation of domesticated wheat cultivars were simultaneous with the duplication of this gene. These results directly support the notion that rearrangement plays a significant role in driving plant mitochondrial genome evolution. C1 [Soltani, Ali; Mergoum, Mohamed; Hegstad, Justin] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Ghavami, Farhad] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Noyszewski, Andrzej] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Meinhardt, Steven] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Kianian, Shahryar F.] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kianian, SF (reprint author), Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM shahryar.kianian@ars.usda.gov FU Monsanto Beachell-Borlaug International Scholars Program FX We would like to thank S.S. Maan and K. Tsunewaki for the development of the alloplasmic wheat lines and providing us the material used in this study. We are also grateful for all the assistance received from members of the Wheat Germplasm Enhancement and the Hard Red Spring Wheat programs at North Dakota State University. Particular thanks to Philip McClean, Justin Faris, and Steven Xu for their valuable suggestions that improved the manuscript. S.F.K. was supported by the Monsanto Beachell-Borlaug International Scholars Program. NR 64 TC 1 Z9 1 U1 0 U2 3 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD MAY PY 2014 VL 57 IS 5 BP 279 EP 288 DI 10.1139/gen-2014-0024 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AP1KB UT WOS:000341826900005 PM 25170648 ER PT J AU Jabro, JD Iversen, WM Evans, RG Allen, BL Stevens, WB AF Jabro, J. D. Iversen, W. M. Evans, R. G. Allen, B. L. Stevens, W. B. TI Repeated Freeze-Thaw Cycle Effects on Soil Compaction in a Clay Loam in Northeastern Montana SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID AGGREGATE STABILITY; PHYSICAL-PROPERTIES; BULK-DENSITY; TILLAGE; SHRINKAGE; SYSTEMS; YIELD AB In recent years, there has been an increased global concern regarding the impact of soil compaction on crop production and soil quality in modern mechanized agricultural farming systems. Farm equipment is heavier than ever before, and many farmers have resorted to energy intensive deep tillage to alleviate compaction. Freeze-thaw processes influence the physical properties of soil, primarily soil compaction and structure. A 3-yr field study was established in fall 2009 to investigate the effects of the dynamics of freeze-thaw cycles (FTCs) on soil compaction in a clay loam. Results showed that frequent FTCs over the winter generally alleviated soil compaction at the 0- to 30-cm depth. During the winter of 2009-2010, soil penetration resistance (PR) in compacted treatments that were subject to freezing and thawing conditions was significantly reduced by 73, 68, and 59% at depths of 0 to 10, 10 to 20, and 20 to 30 cm, respectively. In compacted soils that were not subject to freezing, PR was significantly reduced by approximately 50, 60, and 46% at the same respective depths of the soil profile presumably due to the biology of soil and disruptive effects of shrink-swell cycles caused by frequent wetting-drying processes. These results demonstrate that repeated FTCs can alleviate soil compaction and alter soil physical quality. We conclude that FTCs associated with typical winter weather conditions are the most effective and economical way to alleviate soil compaction and improve soil structure through the dynamics of FTCs. C1 [Jabro, J. D.; Iversen, W. M.; Evans, R. G.; Allen, B. L.; Stevens, W. B.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Jabro, JD (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM jay.jabro@ars.usda.gov NR 32 TC 6 Z9 6 U1 6 U2 40 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 737 EP 744 DI 10.2136/sssaj2013.07.0280 PG 8 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100005 ER PT J AU Sequeira, CH Wills, SA Grunwald, S Ferguson, RR Benham, EC West, LT AF Sequeira, Cleiton H. Wills, Skye A. Grunwald, Sabine Ferguson, Richard R. Benham, Ellis C. West, Larry T. TI Development and Update Process of VNIR-Based Models Built to Predict Soil Organic Carbon SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID DIFFUSE-REFLECTANCE SPECTROSCOPY; NEAR-INFRARED SPECTROSCOPY; REGRESSION; MATTER; TOOL AB The large number of samples, time, and cost to assess soil organic C (SOC) with standard procedures has led to the interest in proximal sensing with visible and near-infrared (VNIR) diffuse reflectance spectroscopy. The objectives of the present study were to (i) evaluate the effect of multivariate techniques and spectra preprocessing methods on the performance of VNIR-based models, (ii) evaluate the effect of subsetting datasets to improve the prediction accuracy of models, and (iii) present a systematic iterative model development and update process. There were three datasets: Dataset-1 was used to the initial model development; Dataset-2 was used to revalidate models developed with Dataset-1; Dataset-3 was used to update promising models identified with Dataset-1 and -2. During initial model development with Dataset-1, the dataset was subset in clusters to try to improve model performance. Subsetting datasets did not improve model performance. Revalidating models with Dataset-2 helped to identify the lack of robustness in the initial models. This is related to the increased sample diversity in Dataset-2 compared to Dataset-1 and highlights the importance of continuously updating models to cover more variability. Based on Dataset-1 and 2, promising models were updated with the larger and more diverse Dataset-3. Following this update, the best model had a coefficient of multiple determination (R-2), root mean squared prediction error (RMSPE), and residual prediction deviation (RPD) of 0.95, 2.062, and 4.39%, respectively. Collecting and evaluating data in separate sets allowed models to be revalidated and updated with new independent samples. This continuous process provides robust models to end users. C1 [Sequeira, Cleiton H.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Sequeira, Cleiton H.; Wills, Skye A.; Ferguson, Richard R.; Benham, Ellis C.; West, Larry T.] USDA NRCS, Natl Soil Survey Ctr, Lincoln, NE 68508 USA. [Grunwald, Sabine] Univ Florida, Gainesville, FL 32611 USA. RP Sequeira, CH (reprint author), Koch Agron Serv LLC, 4111 East 37th St North, Wichita, KS 67220 USA. EM cleiton.sequeira@kochind.com FU USDA-NRCS FX This research was sponsored by the USDA-NRCS. The authors would like to thank all staff of the National Soil Survey Center for sampling, analyzing, and managing the data used in this research. NR 33 TC 2 Z9 2 U1 1 U2 12 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 903 EP 913 DI 10.2136/sssaj2013.08.0354 PG 11 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100023 ER PT J AU Sainju, UM Stevens, WB Caesar-TonThat, T AF Sainju, Upendra M. Stevens, William B. Caesar-TonThat, Thecan TI Soil Carbon and Crop Yields Affected by Irrigation, Tillage, Cropping System, and Nitrogen Fertilization SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; LONG-TERM TILLAGE; ORGANIC-MATTER; NO-TILL; RESIDUE; STORAGE; IMPACT; SEQUESTRATION; MANAGEMENT; SEQUENCE AB Management practices are needed to reduce soil C losses from croplands converted from Conservation Reserve Program (CRP) grassland. We evaluated the effects of irrigation, tillage, cropping system, and N fertilization on surface residue and soil organic C (SOC) at the 0- to 85-cm depth in relation to crop yields in a sandy loam soil from 2005 to 2011 in croplands converted from CRP in western North Dakota. Treatments were two irrigation practices (irrigated vs. nonirrigated) as the main plot and six cropping systems [CRP, conventional till malt barley (Hordeum vulgare L.) with N fertilizer (CTBN), conventional till malt barley without N fertilizer (CTBO), no-till malt barley-pea (Pisum sativum L.) with N fertilizer (NTB-P), no-till malt barley with N fertilizer (NTBN), and no-till malt barley without N fertilizer (NTBO)] as the split plot arranged in a randomized complete block with three replications. Soil surface residue amount and C content were greater in CRP and NTBN than the other cropping systems. At 0 to 5 cm, SOC was greater in irrigated CRP, but at 0 to 85 cm it was greater in nonirrigated NTBN than most other treatments. At 0 to 20 cm, SOC increased by 0.26 to 1.21 Mg C ha(-1) yr(-1) in NTB-P and CRP but decreased by 0.02 to 0.68 Mg C ha(-1) yr(-1) in other cropping systems. Surface residue C and SOC at 0 to 10 cm were related to annualized crop grain yield (R-2 = 0.45-0.77, P <= 0.12, n = 10). Because of positive C sequestration rate and favorable crop yields, NTB-P may be used as a superior management option to reduce soil C losses and sustain yields in croplands converted from CRP in the northern Great Plains. C1 [Sainju, Upendra M.; Stevens, William B.; Caesar-TonThat, Thecan] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Sainju, UM (reprint author), ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov FU USDA-ARS-GRACEnet project FX We greatly appreciate the excellent support provided by Bryan Gebhard and Bill Iversen for the management and irrigation of field plots and Joy Barsotti and Chris Russell for soil and plant sample collection and analysis in the laboratory. We also acknowledge the partial financial support provided by the USDA-ARS-GRACEnet project for this study. NR 50 TC 4 Z9 4 U1 2 U2 18 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 936 EP 948 DI 10.2136/sssaj2013.12.0514 PG 13 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100026 ER PT J AU Moore, EB Wiedenhoeft, MH Kaspar, TC Cambardella, CA AF Moore, E. B. Wiedenhoeft, M. H. Kaspar, T. C. Cambardella, C. A. TI Rye Cover Crop Effects on Soil Quality in No-Till Corn Silage-Soybean Cropping Systems SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER; NITROGEN-FERTILIZATION; GREAT-PLAINS; MANAGEMENT; CARBON; INDICATORS; FRACTIONS; RESIDUE; YIELD; BELT AB Corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] farmers in the upper Midwest are showing increasing interest in winter cover crops. The effects of winter cover crops on soil quality in this region, however, have not been investigated extensively. The objective of this experiment was to determine the effects of a cereal rye (Secale cereale L.) winter cover crop after more than 9 yr in a corn silage-soybean rotation. Four cereal rye winter cover crop treatments were established in 2001: no cover crop, rye after soybean, rye after silage, and rye after both. Soil organic matter (SOM), particulate organic matter (POM), and potentially mineralizable N (PMN) were measured in 2010 and 2011 for two depth layers (0-5 and 5-10 cm) in both the corn silage and soybean phases of the rotation. In the 0- to 5-cm depth layer, a rye cover crop grown after both main crops had 15% greater SOM, 44% greater POM, and 38% greater PMN than the treatment with no cover crops. In general, the treatments that had a rye cover crop after both crops or after corn silage had a positive effect on the soil quality indicators relative to treatments without a cover crop or a cover crop only after soybean. Apparently, a rye cover crop grown only after soybean did not add enough residues to the soil to cause measureable changes in SOM, POM, or PMN. In general, rye cover crop effects were most pronounced in the top 5 cm of soil. C1 [Moore, E. B.; Wiedenhoeft, M. H.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Kaspar, T. C.; Cambardella, C. A.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Kaspar, TC (reprint author), ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM tom.kaspar@ars.usda.gov FU Iowa State University Graduate Program in Sustainable Agriculture; Graduate Minority Assistantship Program FX The authors appreciate the assistance of K. Heikens, B. Knutson, R. Hartwig, Jody Ohmacht, and L. Breuer for establishing the site, collecting data, sample analysis, and managing the field plots. Funding for E.B. Moore's assistantship was provided by the Iowa State University Graduate Program in Sustainable Agriculture and the Graduate Minority Assistantship Program. NR 47 TC 8 Z9 8 U1 6 U2 43 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 968 EP 976 DI 10.2136/sssaj2013.09.0401 PG 9 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100029 ER PT J AU Lentz, RD Lehrsch, GA AF Lentz, Rodrick D. Lehrsch, Gary A. TI Manure and Fertilizer Effects on Carbon Balance and Organic and Inorganic Carbon Losses for an Irrigated Corn Field SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID WATER-QUALITY; MANAGEMENT-PRACTICES; FURROW IRRIGATION; CLAY DISPERSION; GREENHOUSE-GAS; CLIMATE-CHANGE; SOIL-EROSION; RUNOFF; MATTER; STABILITY AB Little is known about inorganic fertilizer or manure effects on organic C (OC) and inorganic C (IC) losses from a furrow irrigated field, particularly in the context of other system C gains or losses. In 2003 and 2004, we measured dissolved organic and inorganic C (DOC, DIC) and particulate OC and IC (POC, PIC) concentrations in irrigation inflow, runoff, and percolation waters (six to seven irrigations per year); C inputs from soil amendments and crop biomass; harvested C; and gaseous C emissions from field plots cropped to silage corn (Zea mays L.) in southern Idaho. Annual treatments included: manure treatment (M) 13 (Year 1) and 34 (Year 2) Mg ha(-1) stockpiled dairy manure; inorganic fertilizer treatment (F) 78 (Year 1) and 195 (Year 2) kg N ha(-1) inorganic N fertilizer; or no amendment treatment (NA) as a control. The mean annual total C input was 15.7, 10.8, and 10.4 Mg ha(-1) for M, F, and NA, respectively, while total C outputs for the three treatments were similar, averaging 12.2 Mg ha(-1). Manure plots ended each growing season with a mean net gain of 3.3 Mg C ha(-1) (a positive net C flux) vs. a net loss for F and NA (-1.6 and -1.5 Mg C ha(-1), respectively). The C added to M was similar to 1.5 x that added to F or NA, yet relative to F, M increased gaseous C emissions only 1.18x, increased runoff DOC losses only 1.04x, decreased particulate runoff total C 19%, and decreased percolate DOC 32%. Increased C gas emissions from manure (relative to fertilizer) were less when silage was removed than when retained (1.18x vs. 2x reported in other studies). This suggests a means by which manure applications to corn crops can be managed to minimize C emissions. Amendments had both direct and indirect influences on individual C components, e. g., the losses of DIC and POC in runoff and DOC in percolation water, producing temporally complex outcomes, which may depend on environmental conditions external to the field. C1 [Lentz, Rodrick D.; Lehrsch, Gary A.] ARS, USDA, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Lentz, RD (reprint author), ARS, USDA, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM rick.lentz@ars.usda.gov NR 63 TC 5 Z9 6 U1 1 U2 26 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 987 EP 1002 DI 10.2136/sssaj2013.07.0261 PG 16 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100031 ER PT J AU Forrestal, P Meisinger, J Kratochvil, R AF Forrestal, Patrick Meisinger, John Kratochvil, Robert TI Winter Wheat Starter Nitrogen Management: A Preplant Soil Nitrate Test and Site-Specific Nitrogen Loss Potential SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID RESIDUAL NITRATE; IRRIGATED CORN; YIELD RESPONSE; SPRING WHEAT; BROMIDE; FERTILIZER; MOVEMENT; QUALITY; GROWTH; RECOMMENDATIONS AB Managing highly variable soil residual nitrate-nitrogen (NO3-N) following corn (Zea mays L.) is difficult because it can supply starter N for winter wheat (Triticum aestivum L.) and/or be leached into water resources during the fall-winter water-recharge season in the humid eastern United States. A series of 65 starter N response studies were conducted in the Coastal Plain and Piedmont regions of Maryland to estimate the soil NO3-N concentration that was both agronomically sufficient and cost effective for wheat. A subset of studies measured soil NO3-N loss (0-60 cm) during the fall-winter season, and additional studies evaluated bromide (Br) to index potential NO3-N leaching. A soil exchange frequency index [ EFI = rainfall * (available soil water capacity)(-1)] was used to identify where NO3-N loss was high and wheat response to starter N was unlikely. A post-planting fall EFI >= 2.5 was associated with NO3-N depletion of > 65% (0-60 cm). A significant (P < 0.001) linear-plateau relationship was found between preplant soil NO3-N concentration (0-30 cm) and wheat grain-yield response to starter N for locations with EFI < 2.5. This agronomic linear-plateau relation was combined with a break-even economic scenario (fertilizer-N cost = grain-response value) to estimate the fall NO3-N sufficiency for wheat, which was 7 mg NO3-N kg(-1) soil (0-30 cm) and corresponded to 9 mg NO3-N kg(-1) soil (0-15 cm). These findings show that a preplant soil NO3-N test for winter wheat can facilitate identification of sites where starter N will produce economic returns and reduce potential NO3-N losses to water resources. C1 [Forrestal, Patrick] TEAGASC, Crops Environm & Land Use, Johnstown Castle, Wexford, Ireland. [Meisinger, John] ARS, USDA, Beltsville, MD 20705 USA. [Kratochvil, Robert] Univ Maryland, College Pk, MD 20742 USA. RP Forrestal, P (reprint author), TEAGASC, Crops Environm & Land Use, Johnstown Castle, Wexford, Ireland. EM patrickjforrestal@gmail.com FU Maryland Grain Producers Utilization Board; Chesapeake Bay Conservation Innovation Grant from the National Fish and Wildlife Foundation FX We gratefully acknowledge partial funding support from the Maryland Grain Producers Utilization Board and a Chesapeake Bay Conservation Innovation Grant from the National Fish and Wildlife Foundation. We thank Dr. Greg McMarthy (USDA-ARS) and Dr. Dean Hively (USGS) for their collaboration at farm sites. We acknowledge the University of Maryland Agricultural Experiment Station managers and staff as well as our producer cooperators for their invaluable assistance in conducting these field experiments. We also thank Ms. Shannon Ingram and Charlotte Schomburg of the USDA-ARS Environmental Management and By-Product Utilization Laboratory staff for assistance with soil analysis. NR 73 TC 2 Z9 2 U1 2 U2 17 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2014 VL 78 IS 3 BP 1021 EP 1034 DI 10.2136/sssaj2013.07.0282 PG 14 WC Soil Science SC Agriculture GA AO7WU UT WOS:000341564100033 ER PT J AU Medina, E Cuevas, E Lugo, AE Terezo, E Jimenez-Osornio, J Macario-Mendoza, PA Montanez, P AF Medina, Ernesto Cuevas, Elvira Lugo, Ariel E. Terezo, Evaristo Jimenez-Osornio, Juan Macario-Mendoza, Pedro Antonio Montanez, Patricia TI CONSERVATIVE NUTRIENT USE BY BIG-LEAF MAHOGANY (Swietenia macrophylla King) PLANTED UNDER CONTRASTING ENVIRONMENTAL CONDITIONS SO REVISTA ARVORE LA English DT Article DE Big-leaf mahogany; Nutrient relations; N-C isotopes ID PUERTO-RICO; ECOLOGY; NITROGEN; FORESTS; GROWTH; LEAVES; SOIL AB We analyzed the nutritional composition and isotope ratios (C and N) of big-leaf mahogany (Swietenia macrophylla King) leaves in plantations established on contrasting soils and climates in Central America (State of Quintana Roo, Yucatan, Mexico) and South America (State of Par, Brazil). The objective was to determine the adaptability of this species to large differences in nutrient availability and rainfall regimes. Nutrient concentrations of leaves and soils were determined spectrophotometrically, and isotope ratios were measured using mass spectrometric techniques. In Par soils were sandier, and acidic, receiving above 2000 mm of rain, whereas in Quintana Roo soils were predominantly clayey, with neutral to alkaline pH due to the underlying calcareous substrate, with about 1300 mm of rain. Leaf area/weight ratio was similar for both sites, but leaves from Quintana Roo were significantly smaller. Average N and K concentrations of adult leaves were similar, whereas Ca concentration was only slightly lower in Par in spite of large differences in Ca availability. Leaves from this site had slightly higher P and lower Al concentrations. Differences in water use efficiency as measured by the natural abundance of C-13 were negligible, the main effect of lower rainfall in Quintana Roo seemed to be a reduction in leaf area. The N isotope signature (delta N-15) was more positive in Para than in Quintana Roo, suggesting higher denitrification rates in the former. Results reveal a calciotrophic behavior and a remarkable capacity of mahogany to compensate for large differences in soil texture and nutrient availability. C1 [Medina, Ernesto] IVIC, Ctr Ecol, San Antonio, Venezuela. [Medina, Ernesto] IITF USDA Forest Serv, San Juan, PR USA. [Cuevas, Elvira] Univ Puerto Rico, Dept Biol, San Juan, PR 00936 USA. [Cuevas, Elvira] Univ Puerto Rico, Ctr Appl Trop Ecol & Conservat, San Juan, PR 00936 USA. [Lugo, Ariel E.] USDA, Forest Serv, San Juan, PR USA. [Terezo, Evaristo] Fazenda Tramontina, Santa Rita Durao, Para, Brazil. [Jimenez-Osornio, Juan; Montanez, Patricia] Univ Autonoma Yucatan, Dept Manejo Recursos Nat, Merida, Venezuela. [Macario-Mendoza, Pedro Antonio] Ctr Invest Quintana Roo, Chetmal, Quintana Roo, Mexico. RP Medina, E (reprint author), IVIC, Ctr Ecol, San Antonio, Venezuela. EM medinage@gmail.com; epcuevas@gmail.com; alugo@fs.fed.us; eterezo@gmail.com; josornio@uady.mx; pmacario@ecosur.qroo.mx; montanez@tunku.uady.mx FU Fundacion Puertoriquena de Conservacion; International Institute of Tropical Forestry (IITF) FX The Fundacion Puertoriquena de Conservacion and the International Institute of Tropical Forestry (IITF) provided funds for E.M. and E.C. to collect soils and plant samples in the field. Mary Jean Sanchez, Edwin Lopez, Maribelis Santiago and Maysaa Ittayem of the IITF laboratory in Puerto Rico processed and analyzed the plant samples, and the soils from Quintana Roo. This research was conducted in cooperation with the University of Puerto Rico. Criticisms of two RARV reviewers improved the original version of this paper. NR 33 TC 0 Z9 0 U1 4 U2 24 PU UNIV FEDERAL VICOSA PI VICOSA PA CAIXA POSTAL 270, VICOSA, MG CEP 36571-00, BRAZIL SN 0100-6762 J9 REV ARVORE JI Rev. Arv. PD MAY-JUN PY 2014 VL 38 IS 3 BP 479 EP 488 PG 10 WC Forestry SC Forestry GA AN6XX UT WOS:000340743200010 ER PT J AU Wetzstein, HY Porter, JA Janick, J Ferreira, JFS AF Wetzstein, Hazel Y. Porter, Justin A. Janick, Jules Ferreira, Jorge F. S. TI FLOWER MORPHOLOGY AND FLORAL SEQUENCE IN ARTEMISIA ANNUA (ASTERACEAE) SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE artemisinin; Artemisia annua; Asteraceae; floral sequence; huang hua hao; pollen presentation; qinghao; Sweet Annie; wormwood ID SELF-INCOMPATIBILITY; L.; PLANTS; TRICHOMES; STIGMA; BIOSYNTHESIS; ISOPRENOIDS; DIVERSITY; SURFACE; MARKER AB Premise of the study: Artemisia annua produces phytochemicals possessing antimalarial, antitumor, anti-inflammatory, and anthelmintic activities. The main active ingredient, artemisinin, is extremely effective against malaria. Breeding to develop cultivars producing high levels of artemisinin can help meet worldwide demand for artemisinin and its derivatives. However, fundamental reproductive processes, such as the sequence of flowering and fertility, are not well understood and impair breeding and seed propagation programs. Methods: Capitulum structure and floral sequence were studied using light and scanning electron microscopy to describe inflorescence architecture, floret opening, and seed set. Key results: Florets are minute and born in capitula containing pistillate ray florets and hermaphroditic disk florets. Ray florets have elongated stigmatic arms that extend prior to disk floret opening. Disk florets exhibit protandry. During the staminate phase, pollen is released within a staminate tube and actively presented with projections at the tip of stigmas as the pistil elongates. During the pistillate phase, stigmatic arms bifurcate and reflex. Stigmas are of the dry type and stain positively for polysaccharides, lipids, and an intact cuticle. Floret numbers vary with genotype, and capitula are predominantly composed of disk florets. Both ray and disk florets produce filled seed. Conclusions: Gynomonoecy, early opening of ray florets, and dichogamy of disk florets promote outcrossing in A. annua. For breeding and seed development, flowering in genotypes can be synchronized under short days according to the floral developmental stages defined. Floret number and percentage seed fill vary with genotype and may be a beneficial selection criterion. C1 [Wetzstein, Hazel Y.; Porter, Justin A.] Univ Georgia, Dept Hort, Athens, GA 30602 USA. [Janick, Jules] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Ferreira, Jorge F. S.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. RP Wetzstein, HY (reprint author), Univ Georgia, Dept Hort, 1111 Miller Plant Sci Bldg, Athens, GA 30602 USA. EM hywetz@uga.edu OI Ferreira, Jorge F.S./0000-0003-4550-6761 NR 37 TC 0 Z9 0 U1 5 U2 33 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 EI 1537-2197 J9 AM J BOT JI Am. J. Bot. PD MAY PY 2014 VL 101 IS 5 BP 875 EP 885 DI 10.3732/ajb.1300329 PG 11 WC Plant Sciences SC Plant Sciences GA AN2YY UT WOS:000340453200014 PM 24812108 ER PT J AU Derksen, RC Altland, JE Oliveira, RB Ozkan, HE AF Derksen, R. C. Altland, J. E. Oliveira, R. B. Ozkan, H. E. TI PERFORMANCE OF GRANULAR COLLECTORS FOR CONTAINER PLANTS AND A COMPARISON OF THE INFLUENCE OF CANOPY TYPE ON PREEMERGENT HERBICIDE DEPOSITS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Container plant; Granules; Preemergent; Spreader; Herbicides; Nurseries AB Preemergent herbicides are commonly used to manage weeds in nurseries that produce container-grown plants. Granular products are applied over the top of a canopy and must pass through the canopy to reach the substrate where weed control is needed. Little guidance is available on methods for measuring the distribution of clay herbicide granules reaching the surface of a container pot substrate. A study was conducted to evaluate three different types of collectors. A glass bottle, sunken in the substrate, provided the most efficient collection of granules released from 76 cm above the substrate surface. The bottle collectors were used to evaluate differences in deposits produced by an air-boom spreader in different sizes of butterfly bush (Buddleja davidii 'Pink Delight') and hydrangea (H. macrophylla 'Niko Blue') canopies. Plant species did not contribute to significant differences in mean collector deposits but did contribute to differences in the variation of deposits under the canopies. Canopy size was a significant factor contributing to differences in mean collector deposits. The variability in granule deposits on the substrate increased with increasing plant size thus increasing the potential for more variability in weed control. C1 [Derksen, R. C.; Altland, J. E.] USDA ARS, Wooster, OH 44691 USA. [Oliveira, R. B.] Univ North Parana, Div Engn & Agr Dev, Bandeirantes, Brazil. [Ozkan, H. E.] Ohio State Univ, Columbus, OH 43210 USA. RP Derksen, RC (reprint author), USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. EM rich.derksen@ars.usda.gov NR 8 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 383 EP 389 PG 7 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000006 ER PT J AU King, BA Winward, TW Bjorneberg, DL AF King, B. A. Winward, T. W. Bjorneberg, D. L. TI COMPARISON OF DROP SIZE AND VELOCITY MEASUREMENTS BY A LASER PRECIPITATION METER AND LOW-SPEED PHOTOGRAPHY OR AN AGRICULTURE SPRINKLER SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Drop size; Drop velocity; Kinetic energy; Laser; Measurement; Photography; Sprinkler irrigation ID IRRIGATION SPRAY NOZZLES; DISTRIBUTIONS; INFILTRATION; IMPACT AB Kinetic energy of water droplets has a substantial effect on development of a soil surface seal and infiltration rate of bare soil. Methods for measuring sprinkler droplet size and velocity needed to calculate droplet kinetic energy have been developed and tested over the past 50 years, each with advantages, disadvantages, and limitations. Drop size and velocity of an impact sprinkler at three operating pressures and one nozzle size were measured using a laser precipitation meter and compared with published values obtained using a photographic method. Significant differences in cumulative volume drop size distributions derived from the two measurement methods were found, especially at the highest operating pressure. Significant differences in droplet velocities were found between measurement methods as well. Significant differences were attributed to differences in minimum drop sizes measured; 0.5 mm for the photographic method versus 0.2 mm for the laser precipitation meter. The laser precipitation meter provided smaller cumulative volume drop size distributions compared to the photographic measurement method The laser precipitation meter tended to provide greater drop velocities which were attributed to altitude differences at experimental sites. The difference in calculated droplet kinetic energy per unit drop volume based on drop and size velocity data from the laser precipitation meter and the photographic method ranged from +12.5 to -28%. The laser precipitation meter generally provided a lower estimate of sprinkler kinetic energy due to the measurement of a greater proportion of smaller drop sizes. Either method can be used to obtain drop size and velocity sprinkler drops needed to calculate sprinkler kinetic energy. The laser precipitation meter requires less skill and labor to measure drop size and velocity. C1 [King, B. A.; Winward, T. W.; Bjorneberg, D. L.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID USA. RP King, BA (reprint author), 3793 N 3600 E, Kimberly, ID USA. EM brad.king@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 413 EP 421 PG 9 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000009 ER PT J AU Harmel, RD Coufal, C Winkler, S Caraway, EA Gentry, T Lee, J AF Harmel, R. D. Coufal, C. Winkler, S. Caraway, E. A. Gentry, T. Lee, J. TI ENVIRONMENTAL IMPACTS OF IN-HOUSE WINDROW COMPOSTING OF BROILER LITTER PRIOR TO LAND APPLICATION IN SUBTROPICAL/SEMI-ARID CONDITIONS SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Compost; Water quality; Odor; Olfactometry; Poultry litter; Waste management ID GAS-CHROMATOGRAPHY-OLFACTOMETRY; WATER-QUALITY DATA; POULTRY LITTER; MANAGEMENT-PRACTICES; RUNOFF; NITROGEN; PHOSPHORUS; PATHOGENS; TEXAS; METABOLISM AB Land application to crop and pasture land is a commonly-applied and effective method of utilizing the resource value of poultry litter. In-house windrow composting of litter is an emerging management practice with the potential to mitigate water quality and nuisance odor concerns associated with land application, but few studies have evaluated these effects. The present study was designed to evaluate the effectiveness of in-house windrow composting to reduce litter bacteria concentrations, improve runoff water quality, and mitigate nuisance odors relative to fresh litter. Results related to bacterial reductions were not definitive due to extremely low Escherichia coli (E. coli) counts in fresh litter prior to in-house windrow composting, which is attributed to dry litter conditions. Likely due to low litter moisture and less than full heating of the windrowed litter, few differences in litter properties or in runoff water quality were observed. In terms of nuisance odor, human monitors reported higher odorant concentrations from the in-house windrow composted litter site, but they noted that the fresh litter application site had a more offensive "manure" smell. Analysis of sorbent tubes also produced inconclusive results related to odor mitigation. Laboratory-based olfactometty, however, determined that the odor detection threshold was almost twice as high (odors were twice as strong) for fresh litter compared to in-house windrow composted litter. In spite of the low moisture content of litter in this study, in-house windrowing of litter prior to land application does appear to have the potential to be an effective litter management practice in terms of environmental impacts, especially reduction of nuisance odors in the subtropical to semi-arid climate of Central Texas. This potential benefit complements other possible benefits such as reduction in food borne pathogens and poultry disease. C1 [Harmel, R. D.] USDA ARS, Temple, TX 76502 USA. [Coufal, C.] Texas AgriLife Extens, Dept Poultry Sci, College Stn, TX USA. [Winkler, S.; Lee, J.] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. [Caraway, E. A.] Weat Texas A&M Univ, Dept Agr, Canyon, TX USA. [Gentry, T.] Texas A&M Univ, Soil & Crop Sci Dept, College Stn, TX USA. RP Harmel, RD (reprint author), USDA ARS, 808 E Blackland Rd, Temple, TX 76502 USA. EM daren.harmel@ars.usda.gov FU Texas State Soil and Water Conservation Board; USEPA through a Clean Water Act FX The authors would like to recognize Larry Koester and Gary Hoeft for sample collection and retrieval, Steven Gottselig for transporting samples to the laboratory, and Emily Martin and Heidi Mjelde for sample analysis. Funding for this project was provided by the Texas State Soil and Water Conservation Board and the USEPA through a Clean Water Act 319(h) grant. This funding supported the research component of the M.S. degree of Scott Winkler, and comprehensive data from this thesis can be found in Winkler (2013). NR 54 TC 2 Z9 2 U1 1 U2 8 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 423 EP 433 PG 11 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000010 ER PT J AU Garcia, RA Stein, SD Piazza, GJ AF Garcia, R. A. Stein, S. D. Piazza, G. J. TI POULTRY BLOOD PRESERVATION AND THE IMPACT OF PRESERVATION ON FLOCCULANT ACTIVITY SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Blood; Hemoglobin; Byproduct; Hydrogen sulfide; Hemolysis; Coagulation; Flocculant ID HYDROGEN-SULFIDE; PROTHROMBIN TIMES; CELLS; MECHANISMS; AGENTS; DAMAGE; EDTA AB Chicken blood is an attractive but problematic raw material for the production of biobased flocculants. Blood begins to degrade as soon as it is collected - it rapidly coagulates, and at longer time scales, the red blood cells lyse and microbial growth results in hydrogen sulfide production. This study investigated the extent to which these types of degradation can be limited by inexpensive chemical treatments, under non-sterile, non-refrigerated conditions. It is shown that while the anticoagulants potassium citrate and potassium oxalate are effective under refrigerated conditions, at ambient temperatures they can only prevent coagulation for about one day. The effectiveness of potassium EDTA, on the other hand, is not as temperature dependent and can prevent coagulation for at least four days at ambient temperature. Similarly, blood treated with oxalate or citrate produces dangerous amounts of hydrogen sulfide, but blood treated with EDTA produces significantly less of the toxic gas. Anticoagulated blood does undergo some red blood cell lysis under the conditions investigated, and a method for limiting this lysis is proposed Finally, it is shown that chicken blood preserved with EDTA can be held in non-refrigerated, non-sterile conditions for at least four days without sacrificing the effectiveness of the flocculant made from the blood. C1 [Garcia, R. A.; Stein, S. D.; Piazza, G. J.] USDA ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Garcia, RA (reprint author), USDA ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM rafael.garcia@ars.usda.gov NR 29 TC 2 Z9 2 U1 2 U2 6 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 445 EP 453 PG 9 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000012 ER PT J AU Purswell, JL Luck, BD Davis, JD AF Purswell, J. L. Luck, B. D. Davis, J. D. TI EFFECT OF AIR DEFLECTORS ON FAN PERFORMANCE IN TUNNEL-VENTILATED BROILER HOUSES WITH A DROPPED CEILING SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Ventilation; Poultry; FANS unit ID HIGH DEW-POINT; GROWTH-RESPONSES; VELOCITY; TEMPERATURES AB Air velocity is a critical design parameter for modern commercial broiler houses, owing to the beneficial effects of increased cooling on live performance and thermal comfort in broiler chickens. As a result, design velocities have increased over the last 15 years and broiler growers have installed additional fan capacity or air deflectors in the ceiling peak to reduce building cross-sectional area. While deflectors have traditionally been recommended only for buildings with open ceilings, an increasing number of growers have installed deflectors in dropped ceiling houses to provide local increases in air velocity without additional fan capacity and improve air velocity uniformity across the house. However, adding additional restriction can increase static pressure and reduce fan performance. Fan flow rates were measured in three tunnel-ventilated commercial broiler houses equipped with air deflectors with the deflectors in the deployed (down) and retracted (up) positions. The broiler houses measured 15.2 x 144.8 m and were equipped with 12 tunnel exhaust fans (137.2 cm diameter) and nine air deflectors at 12.2 m intervals, beginning after the tunnel inlet area and ending 15.2 m before the fans. Flow rate and static pressure data were collected concurrently for each fan for each deflector position with all fans operating to simulate tunnel ventilation during warm weather. Data were analyzed using ANOVA and least square means were separated using Fisher's LSD. The results show that deploying air deflectors significantly reduced mean fan flow rate by 11% (p < 0.0001) and increased static pressure by 11.2 Pa (p < 0.0001). The results of the current study suggest that adding air deflectors is likely not appropriate in existing broiler houses with dropped ceilings when fan performance and energy use are taken into consideration. C1 [Purswell, J. L.] USDA ARS Poultry Res Unit, Mississippi State, MS USA. [Luck, B. D.] Univ Wisconsin, Madison, WI USA. [Davis, J. D.] Mississippi State Univ, Mississippi State, MS 39762 USA. RP Purswell, JL (reprint author), POB 5367, Mississippi State, MS 39762 USA. EM joseph.purswell@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 4 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 471 EP 475 PG 5 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000014 ER PT J AU Seo, YW Yoon, SC Park, B Hinton, A Windham, WR Lawrence, KC AF Seo, Y. W. Yoon, S. C. Park, B. Hinton, A., Jr. Windham, W. R. Lawrence, K. C. TI DEVELOPMENT OF HYPERSPECTRAL IMAGING TECHNIQUE FOR SALMONELLA ENTERITIDIS AND TYPHIMURIUM ON AGAR PLATES SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Hyperspectral imaging; Pathogen detection; Salmonella Enteritidis; Salmonella Typhimurium; Agar plate; Chicken carcass rinse; Background microflora ID POLYMERASE-CHAIN-REACTION; SEROTYPE ENTERITIDIS; UNITED-STATES; QUALITY AB Salmonella is a common cause of foodborne disease resulting from the consumption of contaminated food products: Although a direct plating method is widely used for presumptive positive screening of pathogenic Salmonella colonies on agar plates, it is labor-intensive, time-consuming, and also prone to human errors. This article reports the development of a hyperspectral imaging technique for automated screening of the two common serotypes of Salmonella, Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST), grown on agar plates and for differentiating them from background microflora often found in poultry carcass rinses. Visible near-infrared hyperspectral imaging, providing the spectral and spatial information unique to each microorganism, was utilized to differentiate SE and ST from the background microflora on brilliant green sulfa (BGS) and/or xylose lysine tergitol 4 (XLT4) agar plates. Five different machine-learning algorithms including Mahalanobis distance (MD), k-nearest neighbor (kNN), linear discriminant analysis (LDA), quadratic discriminant analysis (QDA), and support vector machine (SVM) in addition to a multivariate data analysis method, the principal component analysis (PCA), were compared to determine the best classification algorithm in Salmonella detection and classification. When trained on the data from pure cultures of Salmonella and known background microflora, the classification accuracy of each classification algorithm in detecting Salmonella on BGS agar was about 98% on average, although it was difficult to differentiate between SE and ST. The classification accuracy in detecting Salmonella colonies on XL,T4 agar was about 88% on average while the detection accuracy for ST colonies were over 99%. The validation of the classification algorithms with independent test samples of chicken carcass rinses spiked with SE and ST showed that the best performance was achieved by QDA with the prediction accuracy of about 99% (Kappa coefficient=0.97). C1 [Seo, Y. W.; Yoon, S. C.; Park, B.; Hinton, A., Jr.; Windham, W. R.; Lawrence, K. C.] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA. RP Yoon, SC (reprint author), USDA ARS, 950 Coll Stn Rd, Athens, GA 30605 USA. EM seungchul.yoon@ars.usda.gov FU National Institute for Hometown Security KCU [01-09-UK] FX The authors would like to acknowledge Ms. Nasreen Bano, Ms. Jerrie Barnett, Ms. Kimberly Ingram, and Ms. Peggy Feldner for their technical support and assistance from sample preparations and data collection. This research was supported in part by the National Institute for Hometown Security KCU #01-09-UK. NR 29 TC 0 Z9 0 U1 2 U2 11 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD MAY PY 2014 VL 30 IS 3 BP 495 EP 506 PG 12 WC Agricultural Engineering SC Agriculture GA AM7WI UT WOS:000340079000016 ER PT J AU Albuquerque, SSC Rocha, BP Almeida, VM Oliveira, JS Riet-Correa, F Lee, ST Neto, JE Mendonca, FS AF Albuquerque, Samuel S. C. Rocha, Brena P. Almeida, Valdir M. Oliveira, Juceli S. Riet-Correa, Franklin Lee, Stephen T. Neto, Joaquim Evencio Mendonca, Fabio S. TI Cardiac fibrosis associated to the poisoning by Amorimia septentrionalis in cattle SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Poisonous plants; Amorimia septentrionalis; plant poisoning; sudden death; MFA; cardiac fibrosis; cattle ID MONOFLUOROACETATE; PALICOUREA; SHEEP AB Amorimia (Mascagnia) septentrionalls contains sodium monofluoracetate and when consumed by ruminants cause outbreaks of sudden death. This study aimed to describe the epidemiology, clinical and pathological signs of outbreaks of sudden deaths in cattle caused by A. septentrionalis in the states of Pernambuco and Paraiba. For this, technical visits where made on various properties in the regions of Medio Capibaribe/PE and ltabaiana/PB. Eight cattle were necropsied. Tissues were collected from abdominal and thoracic cavities, besides brain and spinal cord. The clinical changes consisted in apathy, prolonged sternal recumbency, reluctance to move, fatigue, tachypnea, tachycardia and positive venous pulse. The animals that were forced to move showed instability, muscular tremors and then a single fall followed by vocalizations, paddling and death in 5-7 minutes. Macroscopic changes consisted in pulmonary edema, globular heart with whitish areas, petechiae and ecchymosis in the epicardium, myocardium and papillary muscles. Microscopically there was an increase of eosinophilia of cytoplasm of cardiomyocytes, picnosis, cariorrexia, karyolysis, loss of striations and multifocal areas of cardiac fibrosis. In the kidney, there was hydropic vacuolar degeneration and necrosis of epithelial cells in convoluted tubules. The clinical signs presented by the cattle poisoned were similar to those previously described by plants containing MFA. The macroscopic and microscopic lesions described in the heart and kidneys are of great diagnostic value. A. septentrionalis is the main toxic plant of livestock interests in the studied regions due to direct and indirect economic losses in livestock that causes. C1 [Albuquerque, Samuel S. C.; Rocha, Brena P.; Almeida, Valdir M.] Univ Fed Rural Pernambuco UFRPE, Dept Vet Med, Programa Posgrad Ciencia Vet, BR-52171900 Recife, PE, Brazil. [Oliveira, Juceli S.] Univ Fed Rural Pernambuco, DMFA, Programa Posgrad Ciencia Anim Trop, BR-52171900 Recife, PE, Brazil. [Riet-Correa, Franklin] Univ Fed Campina Grande, Hosp Vet, CSTR, BR-58700000 Patos de Minas, PB, Brazil. [Lee, Stephen T.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Neto, Joaquim Evencio; Mendonca, Fabio S.] Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Lab Diagnost Anim, BR-52171900 Recife, PE, Brazil. RP Mendonca, FS (reprint author), Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Lab Diagnost Anim, BR-52171900 Recife, PE, Brazil. EM fabio.mendonca@pq.cnpq.br NR 21 TC 0 Z9 0 U1 0 U2 12 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X EI 1678-5150 J9 PESQUI VET BRASIL JI Pesqui. Vet. Bras. PD MAY PY 2014 VL 34 IS 5 BP 433 EP 437 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AM8HX UT WOS:000340115300008 ER PT J AU Gilley, JE Boone, GD Marx, DB AF Gilley, J. E. Boone, G. D. Marx, D. B. TI NUTRIENT CONCENTRATIONS OF RUNOFF AS AFFECTED BY THE DIAMETER OF UNCONSOLIDATED MATERIAL FROM FEEDLOT SURFACES SO TRANSACTIONS OF THE ASABE LA English DT Article DE Beef cattle; Feedlots; Manure management; Manure runoff; Nitrogen movement; Nutrient losses; Phosphorus; Runoff Water quality; Water quality management ID SPATIAL VARIATIONS; CATTLE FEEDLOTS; MANAGEMENT; TRANSPORT; QUALITY; WATER; SOIL; RAINFALL; MANURE; DIET AB Beef cattle feedlots contain unconsolidated material that accumulates on the feedlot surface during a feeding cycle. This study was conducted to measure the effects of varying diameters of unconsolidated surface material and varying flow rates on nutrient concentrations in runoff Unconsolidated surface material with an average diameter of 4.76, 9.53, 19.1, or 47.5 mm and a composite sample with a 15.2 mm mean diameter were placed within 0.75 m wide x 4.0 m long plot areas. Flow was then introduced at the top of the plots in successive increments, and runoff samples for water quality analyses were obtained Particle diameter significantly influenced runoff concentrations of dissolved phosphorus (DP), particulate phosphorus (PP), total phosphorus (TP), NH4-N, and solids transport. Concentrations of DP, PP, TP, and NH4-N for the composite material were 1.90, 1.28, 3.18, and 3.81 mg L-1, respectively, and solids transport was 19.8 g min(-1). Runoff rate significantly affected concentrations of DP, PP, TP, NH4-N, and solids transport for each of the particle size classes except the 4.76 mm diameter material. For the composite material, concentrations of DP, PP, TP, and NH4-N decreased from 4.30 to 0.34 mg L-1, from 5.52 to 0.41 mg L-1, from 9.82 to 0.75 mg L-1, and from 25.8 to 0.49 mg L-1, respectively, as runoff rate increased from 0.02 to 1.10 L s(-1). Nutrient concentrations of runoff from feedlot surfaces are affected by both varying diameters of unconsolidated surface material and varying flow rates. C1 [Gilley, J. E.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Boone, G. D.] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. [Marx, D. B.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. RP Gilley, JE (reprint author), Univ Nebraska, 251 Chase Hall, Lincoln, NE 68583 USA. EM John.Gilley@ars.usda.gov NR 30 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD MAY-JUN PY 2014 VL 57 IS 3 BP 749 EP 759 PG 11 WC Agricultural Engineering SC Agriculture GA AM7XK UT WOS:000340081800006 ER PT J AU Baffaut, C Sadler, EJ Ghidey, F AF Baffaut, C. Sadler, E. J. Ghidey, F. TI A METHODOLOGY TO REDUCE UNCERTAINTIES IN THE HIGH-FLOW PORTION OF A RATING CURVE SO TRANSACTIONS OF THE ASABE LA English DT Article DE Flow; Out-of-bank; Rating curve; Water balance ID WATER-BALANCE; SOIL-MOISTURE; LAND-USE; CLIMATE; MODELS; DISCHARGE AB Streamflow monitoring typically relies on the establishment of a rating curve that describes the relationship between stage and streamflow. This rating curve is based on direct and indirect measurements of discharge at various stages and application of open-channel hydraulics principles. Potential measurement errors increase with out-of-bank flow conditions because of difficulties in measuring flow velocity and flow depth during increased stages. With increasing frequency of large rain events attributed to climate change, it becomes critical to have an accurate rating curve for flood flows. This article presents techniques that, when used concurrently, can help identify errors and reduce uncertainty in the rating curve. They include commonly used techniques such as comparison of mean annual discharge per unit drainage area with those from nearby watersheds having similar characteristics. In addition, new techniques are proposed based on the hydrologic budget of a storm event and the analysis of the change in flow relative to time and stage. The methodology was tested using flow data from the 72 km(2) Goodwater Creek Experimental Watershed in northeast Missouri. Results showed that the original rating curve overestimated flow values by 30% for out-of-bank conditions. A revised rating curve was proposed based on channel conveyance. This revised rating curve satisfied the requirements of water balance and change in flow relative to time and stage. The resulting annual flows were reduced by up to 30%, better matching those of nearby watersheds. These techniques are useful for retrospective analysis of flow data series and for identifying rating curve inconsistencies. C1 [Baffaut, C.; Sadler, E. J.; Ghidey, F.] USDA ARS, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. RP Baffaut, C (reprint author), Univ Missouri, 241 Agr Engn Bldg, Columbia, MO 65211 USA. EM Claire.baffaut@ars.usda.gov FU USDA-NRCS under the CEAP project FX This research was conducted under the USDA-ARS project "Missouri Conservation Effects Assessment Project," which was part of the ARS CEAP-Watershed Assessment Study. Some funding was also provided by the USDA-NRCS under the CEAP project. USDA-ARS support staff, including Aaron Beshears, Mark Olson, Scott Drummond, and Teri Oster, are gratefully acknowledged for their efforts in installing and maintaining the gauging stations as well as collecting and managing the data. NR 33 TC 1 Z9 1 U1 1 U2 5 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD MAY-JUN PY 2014 VL 57 IS 3 BP 803 EP 813 PG 11 WC Agricultural Engineering SC Agriculture GA AM7XK UT WOS:000340081800010 ER PT J AU Nichols, MH Anson, E Keefer, T AF Nichols, M. H. Anson, E. Keefer, T. TI UNCERTAINTY IN MEASURING RUNOFF FROM SMALL WATERSHEDS USING INSTRUMENTED OUTLET PONDS SO TRANSACTIONS OF THE ASABE LA English DT Article DE Data collection; Data processing; Discharge monitoring; Ephemeral runoff Semi-arid AB This study quantified the uncertainty associated with event runoff quantity monitored at watershed outlet ponds. Inflow and outflow depth data were collected from 2004 to 2011 at seven instrumented monitoring stations at the outlet of watersheds ranging in size from 35.2 to 159.5 ha on the USDA-ARS Walnut Gulch Experimental Watershed in southeastern Arizona. The effects of instrumentation, field methods, and data processing procedures were considered. Uncertainty was assessed separately for runoff that did not exceed pond capacity and for runoff that exceeded pond capacity and was discharged through overflow spillways. The largest relative measurement uncertainty was associated with runoff volumes that were less than 50 m(3) in magnitude and with events exceeding pond capacity and discharging over the spillway. The largest source of measurement uncertainty was associated with error in establishing the position of the depth sensor relative to the pond spillway hydraulic control elevation. This analysis is of practical importance for improving field methods for measurement of pond inflow and outflow and was conducted to encourage similar analyses of flow data collected from all monitored watersheds. C1 [Nichols, M. H.; Anson, E.; Keefer, T.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. RP Nichols, MH (reprint author), 2000 E Allen Rd, Tucson, AZ 85719 USA. EM mary.nichols@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD MAY-JUN PY 2014 VL 57 IS 3 BP 851 EP 859 PG 9 WC Agricultural Engineering SC Agriculture GA AM7XK UT WOS:000340081800014 ER PT J AU Patwa, A Ambrose, RPK Dogan, H Casada, ME AF Patwa, A. Ambrose, R. P. Kingsly Dogan, H. Casada, M. E. TI WHEAT MILL STREAM PROPERTIES FOR DISCRETE ELEMENT METHOD MODELING SO TRANSACTIONS OF THE ASABE LA English DT Article DE Moisture content; Physical properties; Wheat flour; Wheat mill streams ID PARTICLE-SIZE DISTRIBUTION; MECHANICAL-PROPERTIES; PHYSICAL-PROPERTIES; MOISTURE-CONTENT; ENDOSPERM; GRAINS; COEFFICIENT; DENSITY; KERNELS; RESTITUTION AB A discrete phase approach based on individual wheat kernel characteristics is needed to overcome the limitations of previous statistical models and accurately predict the milling behavior of wheat. As a first step to develop a discrete element method (DEM) model for the wheat milling process, this study determined the physical and mechanical properties of wheat mill streams (wheat kernels, break stream, and wheat flour) required as input parameters. The parameters measured were particle size and size distribution, bulk density, Young's modulus, static and rolling coefficients of friction, and coefficient of restitution. The effect of moisture content (12% to 16% wet basis) on these properties was evaluated The density, Young's modulus, and coefficient of restitution tended to decrease while the coefficients of friction tended to increase with increasing moisture content of wheat kernels. The effect of moisture content on material properties was significant for break stream, but there was no significant (p > 0.05) material property change with moisture content for flour. It was concluded that moisture content had a greater significant effect on physical properties (bulk, true, and tapped densities and particle size) of the mill streams than it did on the mechanical properties (Young's modulus, coefficients of static and rolling friction, and coefficient of restitution). C1 [Patwa, A.; Ambrose, R. P. Kingsly; Dogan, H.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Casada, M. E.] USDA ARS, Ctr Grain & Anim Hlth Res, Engn & Wind Eros Res Unit, Manhattan, KS USA. RP Ambrose, RPK (reprint author), Kansas State Univ, 312 Shellenberger Hall, Manhattan, KS 66506 USA. EM kingsly@ksu.edu RI Ambrose, Rose Prabin Kingsly/C-4816-2013 NR 43 TC 5 Z9 5 U1 0 U2 4 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD MAY-JUN PY 2014 VL 57 IS 3 BP 891 EP 899 PG 9 WC Agricultural Engineering SC Agriculture GA AM7XK UT WOS:000340081800018 ER PT J AU Park, B Windham, WR Ladely, SR Gurram, P Kwon, H Yoon, SC Lawrence, KC Narang, N Cray, WC AF Park, B. Windham, W. R. Ladely, S. R. Gurram, P. Kwon, H. Yoon, S. C. Lawrence, K. C. Narang, N. Cray, W. C. TI DETECTION OF NON-O157 SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) SEROGROUPS WITH HYPERSPECTRAL MICROSCOPE IMAGING TECHNOLOGY SO TRANSACTIONS OF THE ASABE LA English DT Article DE Acousto-optic tunable filter; Bacteria; Dark-field illumination; Escherichia coli; Foodborne pathogen; Hyperspectral; Microscopy; STEC ID OUTBREAKS; SAFETY; SYSTEM; SKIN AB Non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups such as 026, 045, 0103, 0111, 0121, and 0145 are recognized as serious risks to human health due to their toxicity. The conventional microbiological detection method of cell counting on agar plates is laborious and time-consuming. Because optical methods are promising for real-time, in situ foodborne pathogen detection, a hyperspectral microscope imaging (HMI) method based on acousto-optic tunable filters (AOTF) was developed for detecting pathogenic bacteria with a capability to differentiate the spectral characteristics of each bacterial cell from microcolony samples. Using the AOTF-based HMI method, a total of 89 contiguous spectral images were acquired within approximately 45 s with 250 ms exposure time. In this study, we developed a protocol for successfully immobilizing live cells on glass slides to acquire quality spectral images of STEC bacterial cells using a modified drying method. Among the contiguous spectral imagery between 450 and 800 nm, the intensities at 458, 498, 522, 546, 570, 586, 670, and 690 nm were distinct for STEC bacteria under dark-field illumination. With two different classification algorithms, i.e., support vector machine (SVM) and sparse kernel-based ensemble learning (SKEL), STEC serogroup 045 could be classified with 92% detection accuracy. However, the mean accuracies in identifying the six STEC serogroups with SVM and SKEL were not high enough for use in classification models. C1 [Park, B.; Windham, W. R.; Yoon, S. C.; Lawrence, K. C.] USDA ARS, Russell Res Ctr, Athens, GA 30613 USA. [Ladely, S. R.; Narang, N.; Cray, W. C.] USDA FSIS Eastern Lab, Athens, GA USA. [Gurram, P.; Kwon, H.] US Army Res Lab, Adelphi, MD USA. RP Park, B (reprint author), Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30604 USA. EM bosoon.park@ars.usda.gov FU National Institute for Hometown Security, Somerset, Kentucky FX The authors would like to thank Dr. Nasreen Bano, Jerrie Barnett, Gerald Heitschmidt, and Peggy Feldner of the USDA-ARS Quality and Safety Assessment Research Unit in Athens, Georgia, for their assistance in this research. This project is partially supported by the National Institute for Hometown Security, Somerset, Kentucky. NR 28 TC 3 Z9 3 U1 0 U2 7 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 2151-0032 EI 2151-0040 J9 T ASABE JI Trans. ASABE PD MAY-JUN PY 2014 VL 57 IS 3 BP 973 EP 986 PG 14 WC Agricultural Engineering SC Agriculture GA AM7XK UT WOS:000340081800026 ER PT J AU Friedl, MA Gray, JM Melaas, EK Richardson, AD Hufkens, K Keenan, TF Bailey, A O'Keefe, J AF Friedl, Mark A. Gray, Josh M. Melaas, Eli K. Richardson, Andrew D. Hufkens, Koen Keenan, Trevor F. Bailey, Amey O'Keefe, John TI A tale of two springs: using recent climate anomalies to characterize the sensitivity of temperate forest phenology to climate change SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate change; temperate forests; phenology ID TERRESTRIAL BIOSPHERE MODEL; NORTHERN HARDWOOD FOREST; RESPONSES; FEEDBACKS; PERFORMANCE AB By the end of this century, mean annual temperatures in the Northeastern United States are expected to warm by 3-5 degrees C, which will have significant impacts on the structure and function of temperate forests in this region. To improve understanding of these impacts, we exploited two recent climate anomalies to explore how the springtime phenology of Northeastern temperate deciduous forests will respond to future climate warming. Specifically, springtime temperatures in 2010 and 2012 were the warmest on record in the Northeastern United States, with temperatures that were roughly equivalent to the lower end of warming scenarios that are projected for this region decades from now. Climate conditions in these two years therefore provide a unique empirical basis, that complements model-based studies, for improving understanding of how northeastern temperate forest phenology will change in the future. To perform our investigation, we analyzed near surface air temperatures from the United States Historical Climatology Network, time series of satellite-derived vegetation indices from NASA's Moderate Resolution Imaging Spectroradiometer, and in situ phenological observations. Our study region encompassed the northern third of the eastern temperate forest ecoregion, extending from Pennsylvania to Canada. Springtime temperatures in 2010 and 2012 were nearly 3 degrees C warmer than long-term average temperatures from 1971-2000 over the region, leading to median anomalies of more than 100 growing degree days. In response, satellite and ground observations show that leaf emergence occurred up to two weeks earlier than normal, but with significant sensitivity to the specific timing of thermal forcing. These results are important for two reasons. First, they provide an empirical demonstration of the sensitivity of springtime phenology in northeastern temperate forests to future climate change that supports and complements model-based predictions. Second, our results show that subtle differences in the character of thermal forcing can substantially alter the timing of leaf emergence and canopy development. By explicitly comparing and contrasting the timing of thermal forcing and leaf phenology in 2010 and 2012, we show that even though temperatures were warmer in 2012 than in 2010, the nature and timing of thermal forcing in 2010 lead to leaf emergence that was almost a week earlier than 2012. C1 [Friedl, Mark A.; Gray, Josh M.; Melaas, Eli K.; Hufkens, Koen] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Richardson, Andrew D.; Hufkens, Koen; Keenan, Trevor F.] Biol Harvard Univ, Dept Organism & Evolutionary, Cambridge, MA 02138 USA. [Keenan, Trevor F.] Macquarie Univ, Dept Biol Sci, N Ryde, NSW 2109, Australia. [Bailey, Amey] US Forest Serv, Hubbard Brook Expt Forest, Woodstock, NH 03262 USA. [O'Keefe, John] Harvard Forest, Petersham, MA 01366 USA. RP Friedl, MA (reprint author), Boston Univ, Dept Earth & Environm, 675 Commonwealth Ave, Boston, MA 02215 USA. EM friedl@bu.edu RI Richardson, Andrew/F-5691-2011; Keenan, Trevor/B-2744-2010; OI Richardson, Andrew/0000-0002-0148-6714; Keenan, Trevor/0000-0002-3347-0258; Hufkens, Koen/0000-0002-5070-8109 FU Harvard; NASA [NNX11AE75G]; NSF [EAR-1038907, EF-1065029, DEB-1237491, DEB-1114804] FX The research described in this paper was largely conducted while the lead author was a Charles F Bullard Fellow at Harvard University, and support from Harvard is gratefully acknowledged. In addition, support from NASA grant number NNX11AE75G and NSF grant numbers EAR-1038907, EF-1065029, DEB-1237491, and DEB-1114804 are also gratefully acknowledged. NR 37 TC 15 Z9 16 U1 8 U2 56 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAY PY 2014 VL 9 IS 5 AR 054006 DI 10.1088/1748-9326/9/5/054006 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AM5GM UT WOS:000339885100007 ER PT J AU Kovalchuk, I Zhumagulova, Z Turdiev, T Reed, BM AF Kovalchuk, Irina Zhumagulova, Zhangul Turdiev, Timur Reed, Barbara M. TI GROWTH MEDIUM ALTERATIONS IMPROVE IN VITRO COLD STORAGE OF PEAR GERMPLASM SO CRYOLETTERS LA English DT Article DE germplasm storage; in vitro cold storage; mannitol; micropropagation; nitrogen; Pyrus ID INVITRO AB BACKGROUND: Development of new fruit cultivars is dependent on genetic resource collections such as those at the Pomological Garden of the Institute of Horticulture and Viticulture near Almaty, Kazakhstan. The pear germplasm collection of the Pomological Garden contains 615 cultivars and three species. In vitro cold storage of the collection would provide additional security to the field collection. OBJECTIVE: This study was designed to improve medium-term in vitro storage of pear germplasm. METHODS: Shoots of seven pear cultivars (Pyrus communis L.) were stored in plastic five-section bags at 4 degrees C and a 10-h photoperiod (7 mu mol m(-2) s(-1)). Treatments included medium with four carbohydrate sources (3% sucrose, 2% or 3% mannitol, or 2% sucrose + 2% mannitol) with 0.5 mg l(-1) BAP and 0.1 mg l(-1) IBA or without plant growth regulators (PGRs) and at three Murashige and Skoog (MS) nitrogen concentrations (100%, 50% or 25%). RESULTS: Pear shoots remained viable for 9 to 15 months without repropagation on the control MS medium with 3% sucrose without PGRs. There were significant impacts of cultivar and treatment on the duration of cold storage. Shoots of 'Mramornaya' remained viable (rating of >= 2) for 27 months with PGRs and 2% sucrose + 2% mannitol compared to 12 months for the PGR + 3% sucrose treatment. Talgarskaya Krasaviza' stored for 18 months on 2% sucrose + 2% mannitol while all other treatments lasted only 6 to 9 months. Treatments with 0.5 or 1 mg l(-1) abscisic acid (ABA) with 3% sucrose increased storage duration as did reducing the concentration of nitrogen in the medium to 25% without PGRs and with 3% sucrose. C1 [Kovalchuk, Irina; Zhumagulova, Zhangul; Turdiev, Timur] Inst Plant Biol & Biotechnol, Natl Biotechnol Ctr, Alma Ata 050040, Kazakhstan. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Reed, BM (reprint author), USDA ARS, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM kovalchuk_i_u@mail.ru; Barbara.Reed@ars.usda.gov FU International Science and Technology Center [K428]; USDA Agricultural Research Service CRIS [5358-21000-033D] FX The project was supported by funds from the International Science and Technology Center Grant K428 and the USDA Agricultural Research Service CRIS project 5358-21000-033D. NR 19 TC 1 Z9 1 U1 0 U2 3 PU CRYO LETTERS PI LONDON PA C/O ROYAL VETERINARY COLLEGE, ROYAL COLLEGE ST, LONDON NW1 0TU, ENGLAND SN 0143-2044 EI 1742-0644 J9 CRYOLETTERS JI CryoLetters PD MAY-JUN PY 2014 VL 35 IS 3 BP 197 EP 203 PG 7 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA AL1VA UT WOS:000338913200005 PM 24997837 ER PT J AU Gorski, L Cooley, MB Quinones, B Oryang, D Mandrell, RE AF Gorski, Lisa Cooley, Michael B. Quinones, Beatriz Oryang, David Mandrell, Robert E. TI Prevalence of shigatoxin producing Escherichia coli, Salmonella enterica and Listeria monocytogenes at public access watershed sites in a California Central Coast agricultural region (vol 4, pg 30, 2014) SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Correction DE STEC; salmonella; listeria monocytogenes; watersheds; agriculture C1 [Gorski, Lisa; Cooley, Michael B.; Quinones, Beatriz; Mandrell, Robert E.] ARS, USDA, Albany, CA 94710 USA. [Oryang, David] US FDA, Div Risk Anal, Ctr Food Safety & Appl Nutr, College Pk, MD USA. RP Gorski, L (reprint author), ARS, USDA, Albany, CA 94710 USA. EM lisa.gorski@ars.usda.gov NR 1 TC 1 Z9 1 U1 0 U2 5 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD MAY PY 2014 VL 4 AR 58 DI 10.3389/fcimb.2014.00058 PG 1 WC Immunology; Microbiology SC Immunology; Microbiology GA AL2WR UT WOS:000338986800005 ER PT J AU Hernlem, BJ Ravva, SV Sarreal, CZ AF Hernlem, Bradley J. Ravva, Subbarao V. Sarreal, Chester Z. TI Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous Tetrahymena by flow cytonnetry SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE enteropathogenic E. coli; foodborne pathogen; GFP; flow cytometry; protozoa; environmental transport; Tetrahymena; E. coli O157:H7 ID COLI O157-H7; SALMONELLA-ENTERICA; SURVIVAL; PROTOZOAN; CYTOMETRY; PYRIFORMIS; PATHOGENS; INGESTION; GROWTH; RATES AB Protozoa are known to harbor bacterial pathogens, alter their survival in the environment and make them hypervirulent. Rapid non-culture based detection methods are required to determine the environmental survival and transport of enteric pathogens from point sources such as dairies and feedlots to food crops grown in proximity. Grazing studies were performed on a soil isolate of Tetrahymena fed green fluorescent protein (GFP) expressing Escherichia colt O157:H7 to determine the suitability of the use of such fluorescent prey bacteria to locate and sort bacterivorous protozoa by flow cytometry. In order to overcome autofluorescence of the target organism and to clearly discern Tetrahymena with ingested prey vs. those without, a ratio of prey to host of at least 100:1 was determined to be preferable. Under these conditions, we successfully sorted the two populations using short 5-45 min exposures of the prey and verified the internalization of E. colt O157:H7 cells in protozoa by confocal microscopy. This technique can be easily adopted for environmental monitoring of rates of enteric pathogen destruction vs. protection in protozoa. C1 [Hernlem, Bradley J.] ARS, Foodborne Toxin Detect & Prevent Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Ravva, Subbarao V.; Sarreal, Chester Z.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Ravva, SV (reprint author), ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM subbarao.ravva@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service under CRIS [5325-42000-046, 5325-42000-048] FX Reference to a company or product named by the USDA is only for purposes of information and does not imply approval or recommendation of the product to the exclusion of others that may also be suitable. The work was funded by the U.S. Department of Agriculture, Agricultural Research Service under CRIS projects 5325-42000-046 and 5325-42000-048. We thank Anne Bates for confocal microscopy. NR 21 TC 1 Z9 1 U1 1 U2 14 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD MAY PY 2014 VL 4 AR 57 DI 10.3389/fcimb.2014.00057 PG 5 WC Immunology; Microbiology SC Immunology; Microbiology GA AL2WR UT WOS:000338986800004 PM 24847471 ER PT J AU Yang, YJ Ma, C Xu, YJ Wei, Q Imtiaz, M Lan, HB Gao, S Cheng, LN Wang, MY Fei, ZJ Hong, B Gao, JP AF Yang, Yingjie Ma, Chao Xu, Yanjie Wei, Qian Imtiaz, Muhammad Lan, Haibo Gao, Shan Cheng, Lina Wang, Meiyan Fei, Zhangjun Hong, Bo Gao, Junping TI A Zinc Finger Protein Regulates Flowering Time and Abiotic Stress Tolerance in Chrysanthemum by Modulating Gibberellin Biosynthesis SO PLANT CELL LA English DT Article ID CONSTANS-LIKE GENE; ARABIDOPSIS-THALIANA; LOLIUM-TEMULENTUM; LOCUS-T; TRANSGENIC CHRYSANTHEMUM; REPRODUCTIVE DEVELOPMENT; EXPRESSION PROFILES; PLANT DEVELOPMENT; CIRCADIAN CLOCK; LOW-TEMPERATURE AB Flowering time and an ability to tolerate abiotic stresses are important for plant growth and development. We characterized BBX24, a zinc finger transcription factor gene, from Chrysanthemum morifolium and found it to be associated with both flowering time and stress tolerance. Transgenic lines with suppressed expression of Cm-BBX24 (Cm-BBX24-RNAi) flowered earlier than wild-type plants and showed decreased tolerance to freezing and drought stresses. Global expression analysis revealed that genes associated with both photoperiod and gibberellin (GA) biosynthesis pathways were upregulated in Cm-BBX24-RNAi lines, relative to the wild type. By contrast, genes that were upregulated in overexpressing lines (Cm-BBX24-OX), but downregulated in Cm-BBX24-RNAi lines (both relative to the wild type), included genes related to compatible solutes and carbohydrate metabolism, both of which are associated with abiotic stress. Cm-BBX24 expression was also influenced by daylength and GA(4/7) application. Under long days, changes in endogenous GA(1), GA(4), GA(19), and GA(20) levels occurred in young leaves of transgenic lines, relative to the wild type. Regulation of flowering involves the FLOWERING TIME gene, which integrates photoperiod and GA biosynthesis pathways. We postulate that Cm-BBX24 plays a dual role, modulating both flowering time and abiotic stress tolerance in chrysanthemum, at least in part by influencing GA biosynthesis. C1 [Yang, Yingjie; Ma, Chao; Xu, Yanjie; Wei, Qian; Imtiaz, Muhammad; Lan, Haibo; Cheng, Lina; Wang, Meiyan; Hong, Bo; Gao, Junping] China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China. [Gao, Shan; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Gao, Shan; Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Gao, JP (reprint author), China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China. EM gaojp@cau.edu.cn FU National Natural Science Foundation of China [31372094, 31171990, 91217309] FX We acknowledge Plant Scribe (www.plantscribe.com) for careful editing of this article. This work was supported by the National Natural Science Foundation of China (Grants 31372094, 31171990, and 91217309). NR 73 TC 17 Z9 19 U1 5 U2 47 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD MAY PY 2014 VL 26 IS 5 BP 2038 EP 2054 DI 10.1105/tpc.114.124867 PG 17 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA AK9UA UT WOS:000338771700022 PM 24858937 ER PT J AU Zhai, ZY Gayomba, SR Jung, HI Vimalakumari, NK Pineros, M Craft, E Rutzke, MA Danku, J Lahner, B Punshon, T Guerinot, ML Salt, DE Kochian, LV Vatamaniuk, OK AF Zhai, Zhiyang Gayomba, Sheena R. Jung, Ha-il Vimalakumari, Nanditha K. Pineros, Miguel Craft, Eric Rutzke, Michael A. Danku, John Lahner, Brett Punshon, Tracy Guerinot, Mary Lou Salt, David E. Kochian, Leon V. Vatamaniuk, Olena K. TI OPT3 Is a Phloem-Specific Iron Transporter That Is Essential for Systemic Iron Signaling and Redistribution of Iron and Cadmium in Arabidopsis SO PLANT CELL LA English DT Article ID PLASMA-MEMBRANE TRANSPORTER; SACCHAROMYCES-CEREVISIAE; METAL HOMEOSTASIS; OLIGOPEPTIDE TRANSPORTERS; GLUTATHIONE TRANSPORTER; DEFICIENCY RESPONSES; HIGHER-PLANTS; EXPRESSION; THALIANA; TOLERANCE AB Iron is essential for both plant growth and human health and nutrition. Knowledge of the signaling mechanisms that communicate iron demand from shoots to roots to regulate iron uptake as well as the transport systems mediating iron partitioning into edible plant tissues is critical for the development of crop biofortification strategies. Here, we report that OPT3, previously classified as an oligopeptide transporter, is a plasma membrane transporter capable of transporting transition ions in vitro. Studies in Arabidopsis thaliana show that OPT3 loads iron into the phloem, facilitates iron recirculation from the xylem to the phloem, and regulates both shoot-to-root iron signaling and iron redistribution from mature to developing tissues. We also uncovered an aspect of crosstalk between iron homeostasis and cadmium partitioning that is mediated by OPT3. Together, these discoveries provide promising avenues for targeted strategies directed at increasing iron while decreasing cadmium density in the edible portions of crops and improving agricultural productivity in iron deficient soils. C1 [Zhai, Zhiyang; Gayomba, Sheena R.; Jung, Ha-il; Vimalakumari, Nanditha K.; Rutzke, Michael A.; Vatamaniuk, Olena K.] Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14853 USA. [Pineros, Miguel; Craft, Eric; Rutzke, Michael A.; Kochian, Leon V.] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. [Danku, John; Salt, David E.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen AS24 3UU, Scotland. [Lahner, Brett] Purdue Univ, Ctr Plant Environm Stress Physiol, W Lafayette, IN 47907 USA. [Punshon, Tracy; Guerinot, Mary Lou] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. RP Vatamaniuk, OK (reprint author), Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14853 USA. EM okv2@cornell.edu RI Danku, John/C-3477-2014; OI Danku, John/0000-0002-5103-3852; Kochian, Leon/0000-0003-3416-089X; Pineros, Miguel/0000-0002-7166-1848; Zhai, Zhiyang/0000-0003-3181-1773 FU Department of Energy-Geosciences [DE-FG02-92ER14244]; Department of Energy [DE-AC02-98CH10886]; National Science Foundation [MCB-0923731]; Cornell University Agricultural Experiment Station CUAES Hatch [NYC-125433]; Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy [DE- FG02-06ER15809]; U.S. National Science Foundation [IOS 0701119] FX We thank Walter Gassmann (University of Missouri) and Serge Delrot (University of Bordeaux, France) for S. cerevisiae mutant strains; Christopher D. Town (J. Craig Venter Institute) for the YXT2 vector; Julian Schroeder (University of California, San Diego) and David Mendoza-Cozatl (University of Missouri) for discussing the cell-type specificity of OPT3 expression; the Scanlon Lab (Cornell University) for help with Arabidopsis tissue sectioning; and the Turgeon Lab (Cornell University) for discussing phloem exudate collection. SXRF studies were performed at Beamline X26A, National Synchrotron Light Source (NSLS), Brookhaven National Laboratory. X26A is supported by the Department of Energy-Geosciences (DE-FG02-92ER14244 to the University of Chicago-CARS). Use of the NSLS was supported by the Department of Energy under Contract DE-AC02-98CH10886. Work in the Vatamaniuk lab was supported by the National Science Foundation (MCB-0923731) and Cornell University Agricultural Experiment Station CUAES Hatch (NYC-125433). Work in the Guerinot lab was funded by the Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the U.S. Department of Energy through Grant DE- FG02-06ER15809, and work in the Salt lab was funded by the U.S. National Science Foundation through Grant IOS 0701119. NR 73 TC 24 Z9 24 U1 12 U2 64 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD MAY PY 2014 VL 26 IS 5 BP 2249 EP 2264 DI 10.1105/tpc.114.123737 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA AK9UA UT WOS:000338771700036 PM 24867923 ER PT J AU Chen, YS Blanco, M Ji, Q Frei, UK Lubberstedt, T AF Chen, Yongsheng Blanco, Michael Ji, Qing Frei, Ursula Karoline Luebberstedt, Thomas TI Extensive genetic diversity and low linkage disequilibrium within the COMT locus in maize exotic populations SO PLANT SCIENCE LA English DT Article DE Germplasm Enhancement of Maize; Genetic diversity; Linkage disequilibrium; Caffeic acid 3-0-methytransferase ID ACID O-METHYLTRANSFERASE; CELL-WALL DIGESTIBILITY; BROWN-MIDRIB GENES; ZEA-MAYS L.; LIGNIN BIOSYNTHESIS; DNA POLYMORPHISM; DOMESTICATION; MUTATION; SELECTION; SOFTWARE AB The caffeic acid 3-0-methytransferase (COMT) gene is a prime candidate for cell wall digestibility improvement based on the characterization of brown midrib-3 mutants. We compared the genetic diversity and linkage disequilibrium at this locus between exotic populations sampled within the Germplasm Enhancement of Maize (GEM) project and 70 inbred lines. In total, we investigated 55 exotic COMT alleles and discovered more than 400 polymorphisms in a 2.2 kb region with pairwise nucleotide diversity (7r) up to 0.017, much higher than reported re values of various genes in inbred lines. The ratio of nonsynonymous to synonymous SNPs was 3:1 in exotic populations, and significantly higher than the 1:1 ratio for inbred lines. Selection tests detected selection signature in this gene in both pools, but with different evolution patterns. The linkage disequilibrium decay in exotic populations was at least four times more rapid than for inbred lines with r2 >0.1 persisting only up to 100 bp. In conclusion, the alleles sampled in the GEM Project offer a valuable genetic resource to broaden genetic variation for the COMT gene, and likely other genes, in inbred background. Moreover, the low linkage disequilibrium makes this material suitable for high resolution association analyses. (C) 2014 Elsevier Ireland Ltd. All rights reserved. C1 [Chen, Yongsheng; Ji, Qing; Frei, Ursula Karoline; Luebberstedt, Thomas] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Chen, Yongsheng] Iowa State Univ, Interdept Genet Program, Ames, IA 50011 USA. [Blanco, Michael] USDA ARS, Plant Intro Res Unit, Ames, IA 50011 USA. RP Lubberstedt, T (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM thomasl@iastate.edu NR 41 TC 2 Z9 2 U1 1 U2 13 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD MAY PY 2014 VL 221 BP 69 EP 80 DI 10.1016/j.plantsci.2014.02.004 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AL2UE UT WOS:000338980200008 PM 24656337 ER PT J AU Makatiani, JK Le, HK Olson, DM Wackers, FL Takasu, K AF Makatiani, J. K. Le, H. K. Olson, D. M. Waeckers, F. L. Takasu, K. TI An acquired distaste: sugar discrimination by the larval parasitoid Microplitis croceipes (Hymenoptera: Braconidae) is affected by prior sugar exposure SO JOURNAL OF EXPERIMENTAL BIOLOGY LA English DT Article DE Feeding inhibition; Gustatory responses; Sugar feeding ID AMINO-ACIDS; HONEY-BEES; GUSTATORY RESPONSE; COTESIA-GLOMERATA; PHYSIOLOGICAL-STATE; SEARCHING BEHAVIOR; EXTRAFLORAL NECTAR; ANT PREFERENCES; APIS-MELLIFERA; FOOD SOURCES AB We examined gustatory responses of the larval parasitoid Microplitis croceipes to determine whether the adults discriminate among common sugars, including fructose, glucose, maltose and sucrose, found in plants. When given single sugar solutions of sucrose, glucose, fructose and maltose at concentrations of 0.008-2.0 mol l(-1), the estimated concentrations at which 50% of wasps initiated feeding ranged between 0.054 and 0.085 mol l(-1) for sucrose, glucose and fructose, which was significantly lower than for maltose. Wasps showed a strong decrease in feeding time for maltose or fructose following a brief exposure to other sugars, suggesting that wasps can distinguish maltose and fructose from the other sugars tested. The higher acceptance threshold and short feeding time in the case of maltose appears adaptive in light of the relatively poor nutritional quality of the sugar in the longevity trial. The pronounced feeding inhibition seen for fructose following exposure to other sugars is not linked with lower nutritional performance. This feeding inhibition was even seen in wasps that had fed on glucose at the lowest acceptance threshold (0.031 mol l(-1)) and persisted for 24 h. This study is the first to show feeding inhibition of otherwise phagostimulant sugars such as maltose and fructose after gustatory stimulation on other sugars. C1 [Makatiani, J. K.; Le, H. K.] Kyushu Univ, Grad Sch Bioresources & Bioenvironm Sci, Fukuoka 8128581, Japan. [Olson, D. M.] USDA ARS, Crop Protect & Management Unit, Tifton, GA 31793 USA. [Waeckers, F. L.] Univ Lancaster, Lancaster Environm Ctr, Ctr Sustainable Agr, Lancaster LA1 4YQ, England. [Takasu, K.] Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan. RP Takasu, K (reprint author), Kyushu Univ, Fac Agr, Fukuoka 8128581, Japan. EM takasu@brs.kyushu-u.ac.jp RI U-ID, Kyushu/C-5291-2016 FU Japan Society for the Promotion of Science (JSPS) KAKENHI [24658052] FX This work was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI [grant number 24658052]. NR 67 TC 4 Z9 4 U1 0 U2 10 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0022-0949 EI 1477-9145 J9 J EXP BIOL JI J. Exp. Biol. PD MAY PY 2014 VL 217 IS 10 BP 1692 EP 1700 DI 10.1242/jeb.091843 PG 9 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AK6DG UT WOS:000338517700012 PM 24526723 ER PT J AU Sandhu, HS Glaz, B Edme, SJ Davidson, RW Zhao, DL Comstock, JC Gilbert, RA Milligan, SB Hu, CJ Glynn, NC Sood, S McCorkle, K AF Sandhu, Hardev S. Glaz, Barry Edme, Serge J. Davidson, R. Wayne Zhao, Duli Comstock, Jack C. Gilbert, Robert A. Milligan, Scott B. Hu, Chen-Jian Glynn, Neil C. Sood, Sushma McCorkle, Katherine TI Registration of 'CPCL 02-6848' Sugarcane SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID RUST RESISTANCE AB Development of 'CPCL 02-6848' (Reg. No. CV-158, PI 667596), sugarcane (a complex hybrid of Saccharum spp.) was initiated by the United States Sugar Corporation (USSC) and completed by collaborative research of the USDA-ARS, the University of Florida, and the Florida Sugarcane League, Inc. The female parent of CPCL 02-6848 is CL 92-2533. The male parent is not known because the flower of CL 92-2533 was exposed to the pollen of several male flowers (polycross). 'CL 41-223' is the only cultivar in the pedigree of CPCL 02-6848 that was cultivated on a large acreage (87% of total acreage in 1962) in Florida. The major attributes of CPCL 02-6848 include its high yields of cane tonnage in the plant-cane through the second-ratoon crops on both muck and sand soils, its resistance to smut (caused by Ustilago scitaminea Syd. & P. Syd.), brown rust (caused by Puccinia melanocephala Syd. & P. Syd.), and Sugarcane mosaic virus strain E (SCMV), and moderate resistance to leaf scald [caused by Xanthomonas albineans (Ashby) Dowson] and ratoon stunting disease (caused by Leifsonia xyli subsp. xyli Evtsuhenko et al.) in Florida. CPCL 02-6848 was released on 16 Oct. 2012, and it is expected to be cultivated on both muck and sand soils in Florida. C1 [Sandhu, Hardev S.; Gilbert, Robert A.] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Glaz, Barry; Edme, Serge J.; Zhao, Duli; Comstock, Jack C.; Sood, Sushma; McCorkle, Katherine] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. [Davidson, R. Wayne] Florida Sugar Cane League Inc, Clewiston, FL 33440 USA. [Milligan, Scott B.] Monsanto Co, Vegetable Seeds Div, Felda, FL 33930 USA. [Hu, Chen-Jian] US Sugar Corp, Clewiston, FL 33440 USA. [Glynn, Neil C.] Syngenta Seeds Inc, Longmont, CO 80501 USA. RP Sandhu, HS (reprint author), Univ Florida, Everglades Res & Educ Ctr, 3200 E Palm Beach Rd, Belle Glade, FL 33430 USA. EM hsandhu@ufl.edu NR 28 TC 1 Z9 1 U1 1 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 155 EP 161 DI 10.3198/jpr2013.02.0009crc PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400009 ER PT J AU Berg, JE Hofer, P Davis, ES Stougaard, RN Kephart, KD Lamb, PF Wichman, DM Eckhoff, JL Miller, JH Nash, DL Grey, WE Jin, Y Chen, X Bruckner, PL AF Berg, J. E. Hofer, P. Davis, E. S. Stougaard, R. N. Kephart, K. D. Lamb, P. F. Wichman, D. M. Eckhoff, J. L. Miller, J. H. Nash, D. L. Grey, W. E. Jin, Y. Chen, X. Bruckner, P. L. TI Registration of 'SY Clearstone 2CL' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID IMAZAMOX AB 'SY Clearstone 2CL' (Reg. No. CV-1094, PI 668090) hard red winter wheat (Triticum aestivum L.) was developed by the Montana Agricultural Experiment Station and released in September 2012 through a marketing agreement with Syngenta Seeds. SY Clearstone 2CL is a two-gene Clearfield backcross-derivative of 'Yellowstone' (PI 643428) hard red winter wheat with resistance to imazamox herbicide Beyond (BASF Corporation). SY Clearstone 2CL was selected from the cross Yellowstone*4/3/MTCL01158/Teal 11A//'Jagalene' (PI 631376). Yellowstone is a high-yielding hard red winter wheat released by the Montana Agricultural Experiment Station in 2006. MTCL01158 is an unreleased doubled haploid breeding line [TX12588*4/FS2//Tiber (PI 517194)], which served as the source of proprietary mutant allele TaAHASL1D. Proprietary hard red spring wheat germplasm line Teal 11A (BASF Corporation) served as the source of mutant gene TaAHASL1B. Alleles for herbicide resistance were selected during the backcross process using single-nucleotide, allele-specific polymerase chain reaction markers for TaAHASL1D and TaAHASL1B developed by BASF. SY Clearstone 2CL was selected as an F-2:4 line in 2009 following three generations of screening of the backcross population with imazamox herbicide. Results of three years of replicated field testing indicate that SY Clearstone 2CL is similar to recurrent parent Yellowstone for grain yield, most agronomic characteristics, and milling and baking qualities. C1 [Berg, J. E.; Hofer, P.; Nash, D. L.; Grey, W. E.; Bruckner, P. L.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Davis, E. S.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Stougaard, R. N.] Northwestern Agr Res Ctr, Kalispell, MT 59901 USA. [Kephart, K. D.] Southern Agr Res Ctr, Huntley, MT 59037 USA. [Lamb, P. F.] Northern Agr Res Ctr, Havre, MT 59501 USA. [Wichman, D. M.] Cent Agr Res Ctr, Moccasin, MT 59462 USA. [Eckhoff, J. L.] Eastern Agr Res Ctr, Sidney, MT 59270 USA. [Miller, J. H.] Western Triangle Agr Res Ctr, Conrad, MT 59425 USA. [Jin, Y.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Bruckner, PL (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM bruckner@montana.edu FU BASF Corporation; Montana Agricultural Experiment Station; Montana Wheat and Barley Committee; Montana Board of Research and Commercialization FX SY Clearstone 2CL was developed with financial support of the BASF Corporation, Montana Agricultural Experiment Station, the Montana Wheat and Barley Committee, and the Montana Board of Research and Commercialization. The authors wish to acknowledge the technical assistance of Ron Ramsfield, Jackie Kennedy, and Harvey Teslaa. NR 10 TC 0 Z9 0 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 162 EP 164 DI 10.3198/jpr2013.08.0048crc PG 3 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400010 ER PT J AU Haley, SD Johnson, JJ Peairs, FB Stromberger, JA Hudson-Arns, EE Seifert, SA Valdez, VA Kottke, RA Rudolph, JB Bai, GH Chen, XM Bowden, RL Jin, Y Kolmer, JA Chen, MS Seabourn, BW Dowell, FE AF Haley, Scott D. Johnson, Jerry J. Peairs, Frank B. Stromberger, John A. Hudson-Arns, Emily E. Seifert, Scott A. Valdez, Victoria A. Kottke, Rebecca A. Rudolph, Jeff B. Bai, Guihua Chen, Xianming Bowden, Robert L. Jin, Yue Kolmer, James A. Chen, Ming-Shun Seabourn, Bradford W. Dowell, Floyd E. TI Registration of 'Antero' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID F-SP TRITICI; PUCCINIA-STRIIFORMIS; STRIPE RUST; MARKERS; RESISTANCE AB 'Antero' (Reg. No. CV-1093, PI 667743) hard white winter wheat (Triticum aestivum L.) was developed by the Colorado Agricultural Experiment Station and released in August 2012 through a marketing agreement with the Colorado Wheat Research Foundation. In addition to researchers at Colorado State University (CSU), USDA-ARS researchers at Manhattan, KS, St. Paul, MN, and Pullman, WA, participated in its development. Antero was selected from the cross KS01HW152-1/'TAM 111' made in 2003 at Fort Collins, CO. TAM 111 (PI 631352) is a hard red winter wheat cultivar released by Texas A&M University in 2002 with the pedigree 'TAM 107'//TX78V3630/'Centurk 78'/3/TX87V1233. KS01HW152-1 is an experimental line from Kansas State University with the pedigree 'Trego' (PI 612576)/'Betty' (PI 612578) Sib. Antero was selected as an F-3:4 line in July 2007 and assigned experimental line number CO07W245. Antero was released because of its superior grain yield under nonirrigated and irrigated production conditions in eastern Colorado, its resistance to stripe rust (caused by Puccinia striiformis Westend. f. sp. tritici Eriks.) and stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn), and its milling quality attributes. The name Antero was chosen in recognition of Mount Antero (also known as Antero Peak), one of Colorado's 53 mountains above 4267 m (14,000 ft) elevation. C1 [Haley, Scott D.; Johnson, Jerry J.; Stromberger, John A.; Hudson-Arns, Emily E.; Seifert, Scott A.; Valdez, Victoria A.; Kottke, Rebecca A.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Peairs, Frank B.; Rudolph, Jeff B.] Colorado State Univ, Bioagr Sci & Pest Management Dept, Ft Collins, CO 80523 USA. [Bai, Guihua; Bowden, Robert L.; Chen, Ming-Shun] Kansas State Univ, USDA ARS, Plant Sci & Entomol Res Unit, Manhattan, KS 66506 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Jin, Yue; Kolmer, James A.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Seabourn, Bradford W.; Dowell, Floyd E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Haley, SD (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM scott.haley@colostate.edu FU Colorado Wheat Administrative Committee and Wheat Research Foundation, USDA-CSREES [2009-34205-19960, 2008-34205-19341, 2006-34205-17358, 2005-34205-16334, 2003-34205-13636]; USDA National Institute of Food and Agriculture [2011-68002-30029] FX This research was supported in part by the Colorado Wheat Administrative Committee and Wheat Research Foundation, USDA-CSREES Special Research Grants Nos. 2009-34205-19960, 2008-34205-19341, 2006-34205-17358, 2005-34205-16334, 2003-34205-13636, and the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. NR 21 TC 1 Z9 1 U1 1 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 165 EP 168 DI 10.3198/jpr2013.12.0072crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400011 ER PT J AU Haley, SD Johnson, JJ Peairs, FB Stromberger, JA Hudson-Arns, EE Seifert, SA Kottke, RA Valdez, VA Nachtman, JJ Rudolph, JB Bai, GH Chen, XM Bowden, RL Jin, Y Kolmer, JA Chen, MS Seabourn, BW AF Haley, Scott D. Johnson, Jerry J. Peairs, Frank B. Stromberger, John A. Hudson-Arns, Emily E. Seifert, Scott A. Kottke, Rebecca A. Valdez, Victoria A. Nachtman, Jerry J. Rudolph, Jeff B. Bai, Guihua Chen, Xianming Bowden, Robert L. Jin, Yue Kolmer, James A. Chen, Ming-Shun Seabourn, Bradford W. TI Registration of 'Cowboy' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID WINTER-WHEAT; CL WHEAT; MARKERS AB 'Cowboy' (Reg. No. CV-1095, PI 668564) hard red winter wheat (Triticum aestivum L.) was developed by the Colorado Agricultural Experiment Station and released cooperatively by Colorado State University (CSU) and the University of Wyoming (UWYO) in August 2011. In addition to researchers at CSU and UWYO, USDA-ARS researchers at Manhattan, KS, St. Paul, MN, and Pullman, WA, participated in its development. Cowboy was selected from the cross CO980829/'TAM 111' made in 2001 at Fort Collins, CO. TAM 111 (PI 631352) is a hard red winter wheat cultivar released by Texas A&M University in 2002. CO980829 is an experimental line from CSU with the pedigree 'Yuma' (PI 559720)/PI 372129//CO850034/3/4*Yuma/4/NEWS12. Cowboy was selected as an F-3:4 line in July 2005 and assigned experimental line number CO050322. Cowboy was released because of its superior grain yield and adaptation under nonirrigated and irrigated production systems in Wyoming and its milling and baking quality characteristics. C1 [Haley, Scott D.; Johnson, Jerry J.; Stromberger, John A.; Hudson-Arns, Emily E.; Seifert, Scott A.; Kottke, Rebecca A.; Valdez, Victoria A.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Peairs, Frank B.; Rudolph, Jeff B.] Colorado State Univ, Bioagr Sci & Pest Management Dept, Ft Collins, CO 80523 USA. [Nachtman, Jerry J.] James C Hageman SAREC, Lingle, WY 82223 USA. [Bai, Guihua; Bowden, Robert L.; Chen, Ming-Shun] Kansas State Univ, USDA ARS, Plant Sci & Entomol Res Unit, Manhattan, KS 66506 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Jin, Yue; Kolmer, James A.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Seabourn, Bradford W.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Haley, SD (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM scott.haley@colostate.edu NR 23 TC 0 Z9 0 U1 1 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 169 EP 172 DI 10.3198/jpr2013.12.0075crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400012 ER PT J AU Berg, JE Lamb, PF Miller, JH Wichman, DM Stougaard, RN Eckhoff, JL Kephart, KD Nash, DL Grey, WE Gettel, D Larson, R Jin, Y Kolmer, JA Chen, X Bai, G Bruckner, PL AF Berg, J. E. Lamb, P. F. Miller, J. H. Wichman, D. M. Stougaard, R. N. Eckhoff, J. L. Kephart, K. D. Nash, D. L. Grey, W. E. Gettel, D. Larson, R. Jin, Y. Kolmer, J. A. Chen, X. Bai, G. Bruckner, P. L. TI Registration of 'Warhorse' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Warhorse' (Reg. No. CV-1096, PI 670157) hard red winter (HRW) wheat (Triticum aestivum L.) was developed and released by the Montana Agricultural Experiment Station in September 2013. Warhorse is of unknown pedigree, derived from a composite of three topcrosses made to the same F-1 population in 2000: MT9908//'Nuplains'/MTS9862; Nuplains/MTS9862//MTW0047; and Nuplains/MTS9862//MTS0028. Warhorse was developed using a modified bulk breeding method and selected as an F-5:6 headrow. Warhorse was tested under the experimental number MTS0808 from 2008 to 2013 in Montana. Quality was evaluated in multilocation Montana trials since 2008. Warhorse is a solid-stem, high-yielding HRW wheat line with medium to high test weight, medium maturity, reduced height (Rht-B1b), medium to high grain protein, and acceptable milling and baking quality. Warhorse was released for its improved host plant resistance to wheat stem sawfly (Cephus cinctus Nort.) conditioned by stem solidness, along with short stature and improved yield potential relative to existing solid-stem cultivars adapted to Montana. C1 [Berg, J. E.; Nash, D. L.; Grey, W. E.; Bruckner, P. L.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Lamb, P. F.] Northern Agr Res Ctr, Havre, MT 59501 USA. [Miller, J. H.] Western Triangle Agr Res Ctr, Conrad, MT 59425 USA. [Wichman, D. M.] Cent Agr Res Ctr, Moccasin, MT 59462 USA. [Stougaard, R. N.] Northwestern Agr Res Ctr, Kalispell, MT 59901 USA. [Eckhoff, J. L.] Eastern Agr Res Ctr, Sidney, MT 59270 USA. [Kephart, K. D.] Southern Agr Res Ctr, Huntley, MT 59037 USA. [Gettel, D.] Montana Agr Exptl Stn, Bozeman, MT 59717 USA. [Larson, R.] Montana Seed Growers Assoc, Bozeman, MT 59717 USA. [Jin, Y.; Kolmer, J. A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Bai, G.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Bruckner, PL (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM bruckner@montana.edu FU Montana Agricultural Experiment Station; Montana Wheat and Barley Committee; Hatch [MONB00298] FX Warhorse was developed with financial support of the Montana Agricultural Experiment Station, the Montana Wheat and Barley Committee, and Hatch project MONB00298. The authors wish to acknowledge the technical assistance of Ron Ramsfield, Jackie Kennedy, and Harvey Teslaa. NR 11 TC 0 Z9 0 U1 0 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 173 EP 176 DI 10.3198/jpr2014.01.0001crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400013 ER PT J AU Porch, TG Beaver, JS Abawi, G de Jensen, CE Smith, JR AF Porch, Timothy G. Beaver, James S. Abawi, George de Jensen, Consuelo Estevez Smith, James R. TI Registration of a Small-Red Dry Bean Germplasm, TARS-LFR1, with Multiple Disease Resistance and Superior Performance in Low Nitrogen Soils SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID FUSARIUM ROOT-ROT; QUANTITATIVE TRAIT LOCI; COMMON BACTERIAL-BLIGHT; MACROPHOMINA-PHASEOLINA; SEED YIELD; IDENTIFICATION; MANAGEMENT; FIXATION; NODULATION; RHIZOBIUM AB TARS-LFR1 (Reg. No. GP-292, PI 668258) is a multiple disease resistant common bean (Phaseolus vulgaris L.) germplasm developed through recurrent selection by the USDA-ARS, and by the University of Puerto Rico and Cornell University Agricultural Experiment Stations that has superior performance in low nitrogen (N) soils. Root rots, caused primarily by soil-borne fungi and Oomycetes, are important constraints to common bean production. These diseases are becoming a more serious problem under low-input, low-fertility, and climatic change conditions. In addition to root rot, this germplasm has resistance to common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Smith) Dye, and Bean common mosaic virus and yields well in association with rhizobia through biological nitrogen fixation. This combination of traits could play an important role in low-input and organic production systems. TARS-LFR1 has shown consistently high yield potential under root rot and low soil N conditions, and the use of this improved germplasm by breeding programs or in production zones affected by these stresses can potentially increase yield of common bean. C1 [Porch, Timothy G.] USDA ARS, TARS, Mayaguez, PR 00680 USA. [Beaver, James S.; de Jensen, Consuelo Estevez] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR 00680 USA. [Abawi, George] Cornell Univ, NYSAES, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA. [Smith, James R.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. RP Porch, TG (reprint author), USDA ARS, TARS, 2200 PA Campos Ave.,Suite 201, Mayaguez, PR 00680 USA. EM Timothy.Porch@ars.usda.gov FU USAID Dry Grain Pulses CRSP; Norman Borlaug Cooperative Research Initiative (NBCRI) program FX The authors thank Abraham Montes, Adolfo Quiles, and Edlin Gonzalez for assistance with the field trials. We appreciate the financial support from the USAID Dry Grain Pulses CRSP and from the Norman Borlaug Cooperative Research Initiative (NBCRI) program. NR 45 TC 2 Z9 2 U1 0 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 177 EP 182 DI 10.3198/jpr2013.03.0015crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400014 ER PT J AU Miklas, PN Kelly, JD Steadman, JR McCoy, S AF Miklas, Phillip N. Kelly, James D. Steadman, James R. McCoy, Serena TI Registration of Pinto Bean Germplasm Line USPT-WM-12 with Partial White Mold Resistance SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID IMPROVING PHYSIOLOGICAL RESISTANCE; SELECTION; GAMETE; QTL AB Pinto bean (Phaseolus vulgaris L.), the most widely grown dry bean market class across the United States, is highly susceptible to white mold disease caused by the fungal pathogen Sclerotinia sclerotiorum Lib de Bary. The USDA Agricultural Research Service, Michigan State University AgBioResearch, and the University of Nebraska Agricultural Experiment Station announce the release of USPT-WM-12 (Reg. No. GP-294, PI 668537) pinto bean germplasm line with partial resistance to white mold. USPT-WM-12 was developed by modified pedigree breeding method from the cross G99750/USPT-WM-1 and was tested as PS02-037-2 or 37-2 in early generations. In later generations, USPT-WM-12 exhibited consistently high levels of partial resistance to white mold in field and straw tests conducted by the national Bean White Mold Nursery from 2008 to 2012. USPT-WM-12 also exhibited high yield potential under white mold disease pressure in Michigan, ranking the highest for yield of 64 lines tested for two consecutive years in 2010 and 2011. USPT-WM-12 has commercially acceptable agronomic characteristics, including upright architecture and moderate maturity, but seed appearance is too dark for commercial acceptance. USPT-WM-12 is intended for use by breeders to improve partial resistance to white mold in pinto and related great northern, small red, and pink dry bean market classes representative of Race Durango. C1 [Miklas, Phillip N.] USDA ARS, Vegetable & Forage Crop Res Unit, Prosser, WA 99350 USA. [Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Steadman, James R.; McCoy, Serena] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Miklas, PN (reprint author), USDA ARS, Vegetable & Forage Crop Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM phil.miklas@ars.usda.gov NR 21 TC 6 Z9 6 U1 0 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 183 EP 186 DI 10.3198/jpr2013.06.0034crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400015 ER PT J AU Bell, AA Robinson, AF Quintana, J Dighe, ND Menz, MA Stelly, DM Zheng, XT Jones, JE Overstreet, C Burris, E Cantrell, RG Nichols, RL AF Bell, Alois A. Robinson, A. Forest Quintana, Jose Dighe, Nilesh D. Menz, Monica A. Stelly, David M. Zheng, Xiuting Jones, Jack E. Overstreet, Charles Burris, Eugene Cantrell, Roy G. Nichols, Robert L. TI Registration of LONREN-1 and LONREN-2 Germplasm Lines of Upland Cotton Resistant to Reniform Nematode SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID ROTYLENCHULUS-RENIFORMIS; GOSSYPIUM-LONGICALYX; INTROGRESSION AB Cotton (Gossypium hirsutum L.) germplasm lines LONREN-1 (Reg. No. GP-977, PI 669509) and LONREN-2 (Reg. No. GP-978, PI 669510) were developed and released by the USDA-ARS, Texas Agricultural Experiment Station and Cotton Incorporated in 2007 to provide breeders with desirable germplasm resistant to the reniform nematode (Rotylenchulus reniformis Linford and Oliveira). The resistance was transferred from wild G. longicalyx via a triple-species hybrid. Crosses, backcrosses, and single plant selections were used to develop the F-2 progeny used for seed production. Resistance was followed with the codominant simple sequence repeat BNL 3279_114 marker, located 1.4 cM proximal and the phenotypic marker 'greenfuzz' (LTCOL_F) located 4.5 cM distal to the Ren(lon) resistance gene on chromosome 11. A single nucleotide polymorphism marker also was developed for rapid screening of large numbers of seed. The introgressed chromosome segment is smaller in LONREN-2 than in LONREN-1. Both lines reduced reniform populations by 95% in growth chamber bioassays and by 50 to 90% in fields. In the absence of nematodes in the field, growth rate, yield, and fiber quality of the lines were similar to that of the 'Fibermax 958' parent. In the presence of reniform nematodes in the field, the lines often showed stunting and yield losses, probably due to enhanced severity of fungal seedling diseases, especially Thielaviopsis root rot. The seedling diseases in LONRENs were diminished by control measures such as fungicides, nematicides, and crop rotation with corn or sorghum, and were negligible in a second year of planting in the same field. C1 [Bell, Alois A.; Robinson, A. Forest; Quintana, Jose] USDA ARS, Southern Plains Agr Res Ctr, Cotton Pathol Res Unit, College Stn, TX 77845 USA. [Dighe, Nilesh D.; Menz, Monica A.; Stelly, David M.; Zheng, Xiuting] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Jones, Jack E.] JAJO Genet LLC, Baton Rouge, LA 70808 USA. [Overstreet, Charles] LSU AgCtr, Baton Rouge, LA 70803 USA. [Burris, Eugene] LSU AgCtr, St Joseph, LA 71366 USA. [Cantrell, Roy G.; Nichols, Robert L.] Cotton Inc, Cary, NC 27513 USA. RP Bell, AA (reprint author), USDA ARS, Southern Plains Agr Res Ctr, Cotton Pathol Res Unit, 2765 F&B Rd, College Stn, TX 77845 USA. EM Al.Bell@ars.usda.gov OI Stelly, David/0000-0002-3468-4119 NR 14 TC 7 Z9 7 U1 0 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 187 EP 190 DI 10.3198/jpr2013.11.0069crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400016 ER PT J AU Porter, LD Kraft, JM Grunwald, NJ AF Porter, Lyndon D. Kraft, John M. Gruenwald, Niklaus J. TI Release of Pea Germplasm with Fusarium Resistance Combined with Desirable Yield and Anti-Lodging Traits SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID F-SP PISI; ROOT-ROT; REGISTRATION AB Six F-13 pea (Pisum sativum L.) breeding lines, 00-5001 (Reg. No. GP-108, PI 669372), 00-5003 (Reg. No. GP-109, PI 669373), 00-5004 (Reg. No. GP-110, PI 669374), 00-5005 (Reg. No. GP-111, PI 669375), 00-5006 (Reg. No. GP-112, PI 669376), and 00-5007 (Reg. No. GP-113, PI 669377), were developed by the USDA-ARS, Prosser, WA, via single-seed descent. The lines were developed with traits augmenting yield and harvestability combined with partial resistance to Fusarium root rot caused by Fusarium solani (Mart.) Sacc. f. sp. pisi (van Hall) Snyd. and Hans (Fsp) and resistance to Fusarium wilt caused by Fusarium oxysporum Schlecht. f. sp. pisi (Fop) races 1, 2, and 5. The six lines selected all demonstrated partial resistance to Fsp and resistance to Fop races 1 and 5, while maintaining double or triple pods per node associated with high yields. Four lines also demonstrated resistance to Fop race 2 combined with semi-leafless leaf types, which promote uniform seed color and anti-lodging characteristics. The mean disease severity ratings for Fusarium in all field trials were 50 to 58% less than the susceptible cultivar Bolero. These lines also yielded significantly more than Bolero in field trials in 2008 to 2009. Greenhouse trials confirmed resistances to Fop races 2 and 5 of specific lines. These lines will serve the dry, canning, and freezer pea industries as parents to improve resistance to Fusarium pathogens, which negatively impact the pea industry worldwide. C1 [Porter, Lyndon D.; Kraft, John M.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Gruenwald, Niklaus J.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. RP Porter, LD (reprint author), USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. EM lyndon.porter@ars.usda.gov RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 19 TC 4 Z9 4 U1 2 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 191 EP 194 DI 10.3198/jpr2013.07.0041crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400017 ER PT J AU Jansky, SH Chung, YS Kittipadukal, P AF Jansky, S. H. Chung, Y. S. Kittipadukal, P. TI M6: A Diploid Potato Inbred Line for Use in Breeding and Genetics Research SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID WILD-SPECIES HYBRIDS; INHIBITOR SLI GENE; SELF-COMPATIBILITY; TUBERIZATION; RESISTANCE; LANDRACES; CHACOENSE; DIVERSITY; SEQUENCE; PROGRESS AB M6 (Reg. No. GP-1, BS 228) is a diploid self-compatible inbred line of the potato wild relative Solanum chacoense. It is a vigorous, homozygous breeding line derived by self-pollinating the diploid wild potato relative S. chacoense for seven generations. While most wild Solanum species are self-incompatible, this clone is homozygous for the dominant self-incompatibility inhibitor gene Sli. In addition, it is homozygous for 90% of single-nucleotide polymorphism markers in the Infinium Array developed by the SolCAP consortium. M6 is vigorous and both male and female fertile, producing seeds in crosses to diploid cultivated and wild potato germplasm. These traits enable us to systematically develop diploid inbred lines, which was not possible in potato breeding until the discovery of Sli. M6 produces tubers under both short and long photoperiods, unlike other wild potato relatives. In addition, M6 has several desirable traits, including high dry matter content, good chip processing quality, and resistance to soft rot and Verticillium wilt. M6 is being used to develop recombinant inbred line populations. C1 [Jansky, S. H.] Univ Wisconsin, USDA ARS, Dept Hort, Madison, WI 53706 USA. [Chung, Y. S.] Univ Wisconsin, Madison, WI 53706 USA. [Kittipadukal, P.] Kasetsart Univ, Dept Anat, Bangkok, Thailand. RP Jansky, SH (reprint author), Univ Wisconsin, USDA ARS, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 41 TC 6 Z9 6 U1 5 U2 37 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 195 EP 199 DI 10.3198/jpr2013.05.0024crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400018 ER PT J AU Clark, AJ Costa, JM Griffey, CA Brown-Guedira, GL Dong, YH Souza, EJ Murphy, JP Van Sanford, DA AF Clark, Anthony J. Costa, Jose M. Griffey, Carl A. Brown-Guedira, Gina L. Dong, Yanhong Souza, Edward J. Murphy, J. Paul Van Sanford, David A. TI Registration of Scab-Resistant KY06C-11-3-10 Soft Red Winter Wheat Germplasm SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID HEAD BLIGHT RESISTANCE; SELECTION AB Fusarium head blight (FHB) is a serious disease of wheat (Triticum aestivum L.) worldwide that has elicited a widespread resistance-breeding effort. One approach to breeding relies on deployment of exotic quantitative trait loci (QTL) from wheat outside North America. Germplasm line KY06C-11-3-10 (Reg. No. GP-965, PI 669817) is a soft red winter wheat that is a product of an accelerated backcrossing program performed jointly by the Kentucky Agricultural Experiment Station, Maryland Agricultural Experiment Station, Virginia Polytechnic Institute and State University, North Carolina State University, and the USDA-ARS. KY06C-11-3-10 carries exotic FHB resistance alleles from Chinese spring wheat 'Ning7840' at Fhb1, and at QTL on chromosomes 5A and 2DL. These QTL, backcrossed into 'McCormick'-which itself has nonexotic, native moderate resistance-reduced FHB spike symptoms, percentage Fusarium-damaged kernels, and concentration of the mycotoxin deoxynivalenol (DON). KY06C-11-3-10 has additionally been selected for yield, test weight, heading date, height, milling and baking quality, and resistance to lodging. The combination of exotic resistance QTL, moderately resistant genetic background, widespread adaptation, and competitive agronomic and quality characteristics makes KY06C-11-3-10 a useful germplasm for wheat breeders. C1 [Clark, Anthony J.; Van Sanford, David A.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Costa, Jose M.] Univ Maryland, PSLA Dept, College Pk, MD 20742 USA. [Griffey, Carl A.] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA. [Brown-Guedira, Gina L.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Souza, Edward J.] Bayer Crop Sci, Lincoln, NE USA. [Murphy, J. Paul] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Van Sanford, DA (reprint author), Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. EM dvs@uky.edu FU U.S. Department of Agriculture [59-0206-9-054] FX This material is based on work supported by the U.S. Department of Agriculture, under Agreement No. 59-0206-9-054. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. We thank John Connelley, J. Nicki Mundell, and Sandy Swanson for their technical assistance. NR 14 TC 0 Z9 0 U1 2 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 211 EP 216 DI 10.3198/jpr2013.07.0039crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400020 ER PT J AU Martinez, SA Schramm, EC Harris, TJ Kidwell, KK Garland-Campbell, K Steber, CM AF Martinez, Shantel A. Schramm, Elizabeth C. Harris, Tracy J. Kidwell, Kimberlee K. Garland-Campbell, Kimberly Steber, Camille M. TI Registration of Zak ERA8 Soft White Spring Wheat Germplasm with Enhanced Response to ABA and Increased Seed Dormancy SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GRAIN DORMANCY; SENSITIVITY; TRITICUM; COMPONENTS; TOLERANCE; MUTANTS; EMBRYO AB Zak ERA8 (ENHANCED RESPONSE to ABA8) (Reg. No. GP-966, PI 669443) is a unique line derived from soft white spring wheat (Triticum aestivum L.) cultivar Zak that has increased seed dormancy but after-ripens within 10 to 16 wk. The goal in developing this germplasm was to use increased seed dormancy to improve tolerance to preharvest sprouting, a problem that can cause severe economic losses. This germplasm was developed by USDA-ARS, Pullman, WA, in collaboration with Washington State University. Zak ERA8 was tested under experimental number 60.1.27.10. The ERA8 mutation was generated by chemical mutagenesis followed by selection for the inability to germinate on abscisic acid (ABA) concentrations too low to inhibit wild-type Zak seed germination. The semidominant Zak ERA8 line has been backcrossed twice to wild-type Zak. Following the first backcross, Zak ERA8 showed similar morphological and grain quality traits to the original Zak cultivar. C1 [Martinez, Shantel A.; Schramm, Elizabeth C.; Kidwell, Kimberlee K.; Garland-Campbell, Kimberly; Steber, Camille M.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Martinez, Shantel A.; Schramm, Elizabeth C.; Garland-Campbell, Kimberly; Steber, Camille M.] Washington State Univ, Mol Plant Sci Program, Pullman, WA 99164 USA. [Harris, Tracy J.; Garland-Campbell, Kimberly; Steber, Camille M.] Washington State Univ, USDA ARS, Wheat Genet Physiol Biochem & Qual Unit, Pullman, WA 99164 USA. RP Steber, CM (reprint author), Washington State Univ, Dept Crop & Soil Sci, 209 Johnson Hall, Pullman, WA 99164 USA. EM csteber@wsu.edu FU Washington Grain Commission [5850, 6451]; ARS Project as part of the USDA-ARS National Program 301 [5348-21000-030-00D] FX Thanks are due to R. Parveen, A. Burke, and S. Johnson for expert technical assistance. This research was generously supported by the Washington Grain Commission (5850 and 6451 to CMS and KKK), and by ARS Project Number 5348-21000-030-00D (to CMS and KGC) as part of the USDA-ARS National Program 301. Mention of trade names or commercial products in this registration publication is solely for the purpose of providing information and does not imply recommendation or endorsement by USDA, an equal opportunity provider and employer. NR 14 TC 2 Z9 2 U1 0 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 217 EP 220 DI 10.3198/jpr2013.09.0060crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400021 PM 25580180 ER PT J AU Brown, R Dahleen, LS Lemaux, PG Bregitzer, P AF Brown, Ryan Dahleen, Lynn S. Lemaux, Peggy G. Bregitzer, Phil TI Registration of the Barley Transposon-Tagged Population I: Seventy Lines Each with a Single, Unique Site of Ds Insertion SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID SOMACLONAL VARIATION; EFFICIENT METHOD; GENE; EXPRESSION; TISSUE; GENOME AB The USDA-ARS has developed and released a new genetic stock resource, a transposon-tagging barley (Hordeum vulgare L.) population comprising 70 lines, TNP 200-TNP 207, TNP 210-TNP 229, TNP 232, TNP 233, TNP 235, TNP 237-TNP 262, TNP 264-TNP 267, TNP 269, and TNP 273-TNP 280 (Reg. No. GS-13-GS-82, PI 670045-670114). Each line is homozygous for a single Ds-bar transposable element insertion at a different genomic location. The similar to 3.6-kb Ds-bar element is a modified maize Dissociation (Ds) element containing a bar expression cassette. These lines derive from primary Ds (PDS) transpositions resulting from biolistic cointroduction of Ds-bar and maize Ac transposase (AcT) into 'Golden Promise' (PI 343079). Lines containing secondary, tertiary, and quaternary transpositions were generated by remobilizing Ds-bar via sexual hybridization of AcT-expressing Golden Promise plants with PDS lines and with derived TNP lines containing secondary or tertiary transpositions. Each line was characterized for the 5' and/or 3' sequences flanking the Ds-bar insertion. Insertions that interrupt native genes or regulatory elements likely will alter or abolish the function of these sequences. Therefore, it is expected that these lines will be useful for the study of the biological roles of such genes or regulatory sequences. These lines can also be used as parents for the creation of additional lines with novel transpositions of Ds-bar. C1 [Brown, Ryan; Bregitzer, Phil] USDA ARS, Natl Small Grains Res Facil, Aberdeen, ID 83210 USA. [Dahleen, Lynn S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Lemaux, Peggy G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Bregitzer, P (reprint author), USDA ARS, Natl Small Grains Res Facil, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM phil.bregitzer@ars.usda.gov FU USDA Agricultural Research Service [5366-21000-031-00D] FX This research was funded by the USDA Agricultural Research Service, project 5366-21000-031-00D. The USDA-ARS is an equal opportunity employer. NR 17 TC 0 Z9 0 U1 0 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2014 VL 8 IS 2 BP 226 EP 230 DI 10.3198/jpr2013.09.0056crgs PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AK5CR UT WOS:000338442400023 ER PT J AU Park, SH Bao, ZM Butcher, BG D'Amico, K Xu, Y Stodghil, P Schneider, DJ Cartinhour, S Filiatrault, MJ AF Park, So Hae Bao, Zhongmeng Butcher, Bronwyn G. D'Amico, Katherine Xu, Yun Stodghil, Paul Schneider, David J. Cartinhour, Samuel Filiatrault, M. J. TI Analysis of the small RNA spf in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000 SO MICROBIOLOGY-SGM LA English DT Article ID COMPLETE GENOME SEQUENCE; AMP RECEPTOR PROTEIN; ESCHERICHIA-COLI; NONCODING RNAS; GENE-EXPRESSION; CATABOLITE REPRESSION; PHASEOLICOLA 1448A; ALGINATE SYNTHESIS; FLUORESCENS CHA0; GLOBAL REGULATOR AB Bacteria contain small non-coding RNAs (ncRNAs) that are typically responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they often regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of Pseudomonas syringae DC3000, spot 42 (now referred to as spf), was investigated. A putative RpoE binding site was identified upstream of spf in strain DC3000. RpoE is shown to regulate the expression of spf. Also, deletion of spf results in increased sensitivity to hydrogen peroxide compared with the wild-type strain, suggesting that spf plays a role in susceptibility to oxidative stress. Furthermore, expression of alg8 is shown to be influenced by spf, suggesting that this ncRNA plays a role in alginate biosynthesis. Structural and comparative genomic analyses show this ncRNA is well conserved among the pseudomonads. The findings provide new information on the regulation and role of this ncRNA in P. syringae. C1 [Park, So Hae; Bao, Zhongmeng; Butcher, Bronwyn G.; D'Amico, Katherine; Xu, Yun; Schneider, David J.; Cartinhour, Samuel; Filiatrault, M. J.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [D'Amico, Katherine; Stodghil, Paul; Schneider, David J.; Cartinhour, Samuel; Filiatrault, M. J.] ARS, Plant Microbe Interact Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. RP Filiatrault, MJ (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM melanie.filiatrault@ars.usda.gov RI Schneider, David/H-2236-2012 OI Schneider, David/0000-0002-2124-8385 FU Fredrick N. Gabler '93 Memorial Research Endowment FX We thank Bryan Swingle for contributing to the design of the Delta rpoE mutant. We thank Bryan Swingle and Eric Markel for pBS58 and pBS59. Research support for Y. X. was provided by the Fredrick N. Gabler '93 Memorial Research Endowment. The US Department of Agriculture (USDA) is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purposes of providing specific information and does not imply recommendation or endorsement by the USDA. NR 73 TC 4 Z9 4 U1 0 U2 10 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1350-0872 J9 MICROBIOL-SGM JI Microbiology-(UK) PD MAY PY 2014 VL 160 BP 941 EP 953 DI 10.1099/mic.0.076497-0 PN 5 PG 13 WC Microbiology SC Microbiology GA AK7JB UT WOS:000338603500012 PM 24600027 ER PT J AU Alves, BSQ Dungan, RS Carnin, RLP Galvez, R Pinto, CRSD AF Alves, Barbara S. Q. Dungan, Robert S. Carnin, Raquel L. P. Galvez, Rosa de Carvalho Pinto, Catia R. S. TI Metals in Waste Foundry Sands and an Evaluation of Their Leaching and Transport to Groundwater SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Foundry sand; Groundwater; Leaching; Metals; Probabilistic modeling; TCLP ID GREEN SANDS; REACTIVE MEDIA; PYROLYSIS; PRODUCTS AB While most waste foundry sands (WFSs) are not hazardous, regulatory agencies are often reluctant to permit their beneficial use in agricultural and geotechnical applications due to concerns over metal leaching. The objective of this study was to quantify total and Toxicity Characteristic Leaching Procedure (TCLP) metals in 16 waste sands from Brazilian ferrous foundries then assess their potential to leach to ground-water using a probabilistic model. Total and TCLP metal concentrations in the non-hazardous sands fell within ranges as reported in the literature, although some of the leachate concentrations were found to exceed drinking water and groundwater maximum contaminant levels (MCLs). Leachate values above the MCLs were then used in the model to estimate groundwater concentrations at hypothetical wells up to 400m downgradient from a land application unit. A conservative scenario of 1 ha of land applied WFS, and high annual rainfall totals (low evaporation) suggested that groundwater concentrations of Ba, Hg, Mn, Ni, and Pb could potentially exceed health-based MCLs at most wells. While a wet climate can exacerbate the transport of metals, land application of WFSs in areas with moderate rainfall totals or high rainfall, high evaporation was predicted to be protective of groundwater quality and human health. C1 [Alves, Barbara S. Q.; de Carvalho Pinto, Catia R. S.] Univ Fed Santa Catarina, Depto Engn Sanit & Ambiental, BR-88040970 Florianopolis, SC, Brazil. [Dungan, Robert S.] ARS, USDA, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Carnin, Raquel L. P.] Tupy SA, Joinville, SC, Brazil. [Galvez, Rosa] Univ Laval, Dept Civil Engn, Quebec City, PQ G1V 0A6, Canada. RP Dungan, RS (reprint author), ARS, USDA, Northwest Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. EM robert.dungan@ars.usda.gov NR 34 TC 2 Z9 2 U1 2 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD MAY PY 2014 VL 225 IS 5 AR 1963 DI 10.1007/s11270-014-1963-4 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA AK3OP UT WOS:000338334400026 ER PT J AU Liu, DB Steinberg, CEW Straus, DL Pedersen, LF Meinelt, T AF Liu, Dibo Steinberg, Christian E. W. Straus, David L. Pedersen, Lars-Flemming Meinelt, Thomas TI Salinity, dissolved organic carbon and water hardness affect peracetic acid (PAA) degradation in aqueous solutions SO AQUACULTURAL ENGINEERING LA English DT Article DE Aquaculture; Disinfection; Dosage control; Peracetic acid ID HYDROGEN-PEROXIDE; ICHTHYOPHTHIRIUS-MULTIFILIIS; WASTE-WATER; AQUACULTURE SYSTEMS; DISINFECTION; KINETICS; TOXICITY; DECOMPOSITION; HYDROLYSIS; REDUCE AB Peracetic acid (PAA) is used in aquaculture under different conditions for disinfection and therapeutic purposes. There is limited information about its environmental fate, particularly its persistence in aquatic systems with different physical-chemical conditions. This study investigated PAA-degradation of three commercial products, Wofasteril (R) E400, Wofasteril (R) E250 and Wofasteril (R) Lspez, at a nominal concentration of 1 mg L-1 in relation to two levels of salinity, water hardness, or dissolved organic carbon (DOC). The results showed that salinity and DOC stimulate PAA-degradation, while water hardness had only minor impact. For commercial aquaculture, actual PAA concentration in the raw product needs to be measured; the fate of PAA in individual facilities must be carefully monitored and feasible application strategies need to be investigated to achieve maximal disinfection and therapeutic efficiency. (C) 2014 Elsevier B.V. All rights reserved. C1 [Liu, Dibo] Humboldt Univ, Fac Agr & Hort, D-10115 Berlin, Germany. [Liu, Dibo; Meinelt, Thomas] Leibniz Inst Freshwater Ecol & Inland Fisheries, D-12587 Berlin, Germany. [Steinberg, Christian E. W.] Humboldt Univ, Dept Biol, Lab Freshwater & Stress Ecol, D-12437 Berlin, Germany. [Straus, David L.] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Pedersen, Lars-Flemming] North Sea Res Ctr, Natl Inst Aquat Sci, Aquaculture Sect, DTU Aqua, DK-9850 Hirtshals, Denmark. RP Liu, DB (reprint author), Leibniz Inst Freshwater Ecol & Inland Fisheries, Muggelseedamm 301, D-12587 Berlin, Germany. EM liu@igb-berlin.de RI Pedersen, Lars-Flemming/D-9919-2011 OI Pedersen, Lars-Flemming/0000-0002-8361-9268 FU Schreiner-Foundation for Research and Education FX We wish to thank the Schreiner-Foundation for Research and Education for the financial support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the Leibniz-Institute of Freshwater Ecology and Inland Fisheries, the DTU Aqua, or the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 30 TC 2 Z9 2 U1 2 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8609 EI 1873-5614 J9 AQUACULT ENG JI Aquac. Eng. PD MAY PY 2014 VL 60 BP 35 EP 40 DI 10.1016/j.aquaeng.2014.03.006 PG 6 WC Agricultural Engineering; Fisheries SC Agriculture; Fisheries GA AJ9CJ UT WOS:000338004100006 ER PT J AU Wang, B Wang, RR Cui, ZH Bi, WL Li, JW Li, BQ Ozudogru, EA Volk, GM Wang, QC AF Wang, Biao Wang, Ren-Rui Cui, Zhen-Hua Bi, Wen-Lu Li, Jing-Wei Li, Bai-Quan Ozudogru, Elif Aylin Volk, Gayle M. Wang, Qiao-Chun TI Potential applications of cryogenic technologies to plant genetic improvement and pathogen eradication SO BIOTECHNOLOGY ADVANCES LA English DT Review DE Classical breeding; Cryogenic techniques; Cryotherapy; Genetic engineering; Genetic improvement; Germplasm; Pathogen ID GROWN SHOOT-TIPS; LONG-TERM CRYOPRESERVATION; EMBRYOGENIC-CELL SUSPENSIONS; SOLANUM-TUBEROSUM L.; VITIS-VINIFERA L.; SEED-BORNE POTATO; ORYZA-SATIVA L; ENCAPSULATION-DEHYDRATION; DROPLET-VITRIFICATION; RYE PROTOPLASTS AB Rapid increases in human populations provide a great challenge to ensure that adequate quantities of food are available. Sustainable development of agricultural production by breeding more productive cultivars and by increasing the productive potential of existing cultivars can help meet this demand. The present paper provides information on the potential uses of cryogenic techniques in ensuring food security, including: (1) long-term conservation of a diverse germplasm and successful establishment of cryo-banks; (2) maintenance of the regenerative ability of embryogenic tissues that are frequently the target for genetic transformation; (3) enhancement of genetic transformation and plant regeneration of transformed cells, and safe, long-term conservation for transgenic materials; (4) production and maintenance of viable protoplasts for transformation and somatic hybridization; and (5) efficient production of pathogen-free plants. These roles demonstrate that cryogenic technologies offer opportunities to ensure food security. (C) 2014 Elsevier Inc. All rights reserved. C1 [Wang, Biao; Wang, Ren-Rui; Cui, Zhen-Hua; Bi, Wen-Lu; Li, Jing-Wei; Li, Bai-Quan; Wang, Qiao-Chun] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol & Arid Areas,Minis, Key Lab Genet Improvement Hort Plants Northwest C, Yangling 712100, Shaanxi, Peoples R China. [Ozudogru, Elif Aylin] CNR, IVAISA, Trees & Timber Inst, Natl Res Council, Florence, Italy. [Volk, Gayle M.] USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Wang, QC (reprint author), Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol & Arid Areas,Minis, Key Lab Genet Improvement Hort Plants Northwest C, Yangling 712100, Shaanxi, Peoples R China. EM qiaochunwang@nwsuaf.edu.cn FU Department of Science & Technology of Shaanxi Province through a key project "13115" [2009ZDKG-10]; Department of Fruit Industry of Shaanxi [K336021105]; President Foundation of Northwest AF University; KC Wong Education Foundation from Hong Kong, China FX The authors would like to acknowledge the financial support from the Department of Science & Technology of Shaanxi Province through a key project "13115" (2009ZDKG-10), Department of Fruit Industry of Shaanxi (K336021105), the President Foundation of Northwest A&F University and KC Wong Education Foundation from Hong Kong, China. NR 199 TC 13 Z9 15 U1 1 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0734-9750 EI 1873-1899 J9 BIOTECHNOL ADV JI Biotechnol. Adv. PD MAY-JUN PY 2014 VL 32 IS 3 BP 583 EP 595 DI 10.1016/j.biotechadv.2014.03.003 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AJ7CS UT WOS:000337855400004 PM 24681087 ER PT J AU Forcey, GM Thogmartin, WE Linz, GM McKann, PC AF Forcey, Greg M. Thogmartin, Wayne E. Linz, George M. McKann, Patrick C. TI Land use and climate affect Black Tern, Northern Harrier, and Marsh Wren abundance in the Prairie Pothole Region of the United States SO CONDOR LA English DT Article DE abundance maps; Bayesian; hierarchical models; Markov chain Monte Carlo; North American Breeding Bird Survey; Prairie Pothole Region; wetland birds ID BREEDING BIRD SURVEY; RED-WINGED BLACKBIRDS; GRASSLAND BIRDS; HIERARCHICAL ANALYSIS; CERULEAN WARBLERS; POPULATION-CHANGE; AVIAN ABUNDANCE; LANDSCAPE-SCALE; COVER DATA; MODEL AB Bird populations are influenced by many environmental factors at both large and small scales. Our study evaluated the influences of regional climate and land-use variables on the Northern Harrier (Circus cyaneus), Black Tern (Childonias niger), and Marsh Wren (Cistothorus palustris) in the prairie potholes of the upper Midwest of the United States. These species were chosen because their diverse habitat preference represent the spectrum of habitat conditions present in the Prairie Potholes, ranging from open prairies to dense cattail marshes. We evaluated land-use covariates at three logarithmic spatial scales (1,000 ha, 10,000 ha, and 100,000 ha) and constructed models a priori using information from published habitat associations and climatic influences. The strongest influences on the abundance of each of the three species were the percentage of wetland area across all three spatial scales and precipitation in the year preceding that when bird surveys were conducted. Even among scales ranging over three orders of magnitude the influence of spatial scale was small, as models with the same variables expressed at different scales were often in the best model subset. Examination of the effects of large-scale environmental variables on wetland birds elucidated relationships overlooked in many smaller-scale studies, such as the influences of climate and habitat variables at landscape scales. Given the spatial variation in the abundance of our focal species within the prairie potholes, our model predictions are especially useful for targeting locations, such as northeastern South Dakota and central North Dakota, where management and conservation efforts would be optimally beneficial. This modeling approach can also be applied to other species and geographic areas to focus landscape conservation efforts and subsequent small-scale studies, especially in constrained economic climates. C1 [Forcey, Greg M.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58105 USA. [Thogmartin, Wayne E.; McKann, Patrick C.] US Geol Survey, Upper Midwest Environm Sci Ctr, La Crosse, WI USA. [Linz, George M.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Bismarck, ND USA. RP Forcey, GM (reprint author), Normandeau Associates, Gainesville, FL 32601 USA. EM research@gregforcey.com RI Thogmartin, Wayne/A-4461-2008 OI Thogmartin, Wayne/0000-0002-2384-4279 FU National Wildlife Research Center (NWRC), a unit within the U.S. Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services (WS); Department of Biological Sciences; Graduate School at North Dakota State University FX We thank William J. Bleier, Mario E. Biondini, Gary K. Clambey, and Gary L. Nuechterlein for their contributions to and advice on this study. David Anderson, Diane Granfors, Rex Johnson, Neal Niemuth, and John Sauer provided suggestions on the data analyses for this project. We also thank Eric O'Neal for providing ArcGIS plug-ins to simplify the spatial analyses of climate and land-use data. Thousands of volunteers annually conduct Breeding Bird Survey counts and we are grateful for their efforts. This project was funded by the National Wildlife Research Center (NWRC), a unit within the U.S. Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services (WS). George M. Linz served as study director (WS-NWRC QA-1039). Financial support was also provided by the Department of Biological Sciences and the Graduate School at North Dakota State University. Any use of trade, product, or firm names are for descriptive purposes only and do not imply endorsement by the U.S. Government. NR 66 TC 5 Z9 5 U1 6 U2 42 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD MAY PY 2014 VL 116 IS 2 BP 226 EP 241 DI 10.1650/CONDOR-13-019-R1.1 PG 16 WC Ornithology SC Zoology GA AK0ID UT WOS:000338095200008 ER PT J AU Du, ZJ Wang, Y Dunlap, C Rooney, AP Chen, GJ AF Du, Zong-Jun Wang, Ying Dunlap, Christopher Rooney, Alejandro P. Chen, Guan-Jun TI Draconibacterium orientale gen. nov., sp nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID CHINCHILLA-LANIGERA FECES; REACTOR TREATING WASTE; FLUOROMETRIC METHOD; CATTLE FARMS; SEDIMENT; BACTERIUM; MICROORGANISMS; ANAEROBE AB The taxonomic characteristics of two bacterial strains, FH5(T) and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20-40 degrees C (optimum, 28-32 degrees C), pH 5.5-9.0 (optimum, pH 7.0-7.5) and in the presence of 1-7% NaCl (optimum, 2-4%). The major cellular fatty acids were anteiso-C-15:0 and iso-C-(15:0). Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5(T) and SS4 were both determined to be 42.0 mol%. The results of DNA DNA hybridization studies indicated that the FH5(T) and SS4 genomes share greater than 95% relatedness. The strains formed a distinct phyletic line within the class Bacteroidia, with less than 89.4% sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class Bacteroidia, are proposed, with strain FH5(T) (=DSM 25947(T)=CICC 10585(T)) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov. C1 [Du, Zong-Jun; Wang, Ying; Chen, Guan-Jun] Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China. [Du, Zong-Jun; Chen, Guan-Jun] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China. [Dunlap, Christopher; Rooney, Alejandro P.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Chen, GJ (reprint author), Shandong Univ Weihai, Coll Marine Sci, Weihai 264209, Peoples R China. EM guanjun@sdu.edu.cn FU National Natural Science Foundation of China [31370057, 31290231]; National Science and Technology Major Project of China [2013ZX10004217]; China Ocean Mineral Resources R & D Association (COMRA) Special Foundation [DY125-15-T-05] FX This work was supported by the National Natural Science Foundation of China (31370057, 31290231), National Science and Technology Major Project of China (2013ZX10004217) and the China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-T-05). We thank Dr Jean Euzeby for valuable advice with the nomenclature of the bacterial taxa. NR 31 TC 23 Z9 23 U1 0 U2 5 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD MAY PY 2014 VL 64 BP 1690 EP 1696 DI 10.1099/ijs.0.056812-0 PN 5 PG 7 WC Microbiology SC Microbiology GA AJ8CU UT WOS:000337931100035 PM 24523447 ER PT J AU Susta, L Hamal, KR Miller, PJ Cardenas-Garcia, S Brown, CC Pedersen, JC Gongora, V Afonso, CL AF Susta, Leonardo Hamal, Krishna R. Miller, Patti J. Cardenas-Garcia, Stivalis Brown, Corrie C. Pedersen, Janice C. Gongora, Victor Afonso, Claudio L. TI Separate Evolution of Virulent Newcastle Disease Viruses from Mexico and Central America SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID PROTEIN CLEAVAGE SITE; CLINICOPATHOLOGICAL CHARACTERIZATION; GENETIC DIVERSITY; RECENT OUTBREAKS; POULTRY; GENOTYPES; RNA AB An outbreak of Newcastle disease (ND) in poultry was reported in Belize in 2008. The characteristics of three virulent Newcastle disease virus (NDV) isolates from this outbreak (NDV-Belize-3/08, NDV-Belize-4/08, and NDV-Belize-12/08) were assessed by genomic analysis and by clinicopathological characterization in specific-pathogen-free (SPF) chickens. The results showed that all three strains belong to NDV genotype V and are virulent, as assessed by the intracerebral pathogenicity index and the polybasic amino acid sequence at the fusion protein cleavage site. In 4-week-old SPF chickens, NDV-Belize-3/08 behaved as a typical velogenic viscerotropic NDV strain, causing severe necrohemorrhagic lesions in the lymphoid organs, with systemic virus distribution. Phylogenetic analysis of multiple NDV genotype V representatives revealed that genotype V can be divided into three subgenotypes, namely, Va, Vb, and Vc, and that all tested Belizean isolates belong to subgenotype Vb. Furthermore, these isolates are nearly identical to a 2007 isolate from Honduras and appear to have evolved separately from other contemporary viruses circulating in Mexico, clustering into a new clade within NDV subgenotype Vb. C1 [Susta, Leonardo; Hamal, Krishna R.; Miller, Patti J.; Cardenas-Garcia, Stivalis; Afonso, Claudio L.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. [Hamal, Krishna R.] Univ Georgia, Coll Vet Med, Dept Infect Dis, Athens, GA USA. [Cardenas-Garcia, Stivalis; Brown, Corrie C.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. [Pedersen, Janice C.] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Gongora, Victor] Belize Agr Hlth Author, Dept Anim Hlth, Cayo, Belize. RP Susta, L (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM lsusta1@gmail.com FU USDA [CRIS 6612-32000-064-00D] FX This work was supported by USDA funding (CRIS 6612-32000-064-00D). NR 31 TC 9 Z9 9 U1 1 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAY PY 2014 VL 52 IS 5 BP 1382 EP 1390 DI 10.1128/JCM.00066-14 PG 9 WC Microbiology SC Microbiology GA AJ7XV UT WOS:000337915700012 PM 24523463 ER PT J AU Shatkin, JA Wegner, TH Bilek, EM Cowie, J AF Shatkin, Jo Anne Wegner, Theodore H. Bilek, E. M. (Ted) Cowie, John TI Market projections of cellulose nanomaterial-enabled products - Part 1: Applications SO TAPPI JOURNAL LA English DT Article ID NANOCELLULOSE AEROGELS; COMPOSITES AB Nanocellulose provides a new materials platform for the sustainable production of high-performance nano-enabled products in an array of applications. In this paper, potential applications for cellulose nanomaterials are identified as the first step toward estimating market volume. The overall study, presented in two parts, estimates market volume on the basis of estimated tonnage of cellulose nanomaterials rather than the dollar value of production or profits from production. In this paper, we first identified potential uses from literature, presentations, and patent reviews, and then categorized these under the broad headings of high-volume, low-volume, and emerging/novel applications. For each application, the rationale for using nanocellulose is explained. The companion paper, Part 2, explains the assumptions and calculation of application-specific market estimates. High- and low-volume consumption applications of cellulose nanomaterials were identified from published data as well as expert input. We categorized potential market sizes as high or low by considering applications where cellulose nanomaterials would replace existing materials and be used at a published or estimated rate for some fraction of an entire existing market. Novel applications for cellulose nanomaterials that are presently considered niche markets are also identified, but volumes were not estimated because of a lack of published supporting data. Annual U.S. market potential for identified applications of nanocellulose is estimated as 6.4 million metric tons, with a global market potential of 35 million metric tons. The greatest volume potential for use of cellulose nanomaterials is currently in paper and packaging applications. Other potentially high-volume uses are in the automotive, construction, personal care, and textile sectors. C1 [Shatkin, Jo Anne] Vireo Advisors, Boston, MA 02205 USA. [Wegner, Theodore H.; Cowie, John] USDA, Forest Prod Lab, US Forest Serv, Madison, WI 53705 USA. Cowie & Co, Derwood, MD USA. RP Shatkin, JA (reprint author), Vireo Advisors, Boston, MA 02205 USA. EM jashatkin@gmail.com NR 39 TC 27 Z9 27 U1 2 U2 36 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD MAY PY 2014 VL 13 IS 5 BP 9 EP 16 PG 8 WC Materials Science, Paper & Wood SC Materials Science GA AJ8GI UT WOS:000337940400002 ER PT J AU Chintala, R Schumacher, TE McDonald, LM Clay, DE Malo, DD Papiernik, SK Clay, SA Julson, JL AF Chintala, Rajesh Schumacher, Thomas E. McDonald, Louis M. Clay, David E. Malo, Douglas D. Papiernik, Sharon K. Clay, Sharon A. Julson, James L. TI Phosphorus Sorption and Availability from Biochars and Soil/Biochar Mixtures SO CLEAN-SOIL AIR WATER LA English DT Article DE Acidic soil; Calcareous soil; P desorption; P retention ID PHOSPHATE SORPTION; CALCAREOUS SOILS; DESORPTION; PH; ADSORPTION; FERTILITY; PYROLYSIS; GOETHITE; NITROGEN; CALCIUM AB In an energy-limited world, biomass may be converted to energy products through pyrolysis. A byproduct of this process is biochar. A better understanding is needed of the sorption characteristics of biochars, which can influence the availability of plant essential nutrients and potential water contaminants such as phosphorus (P) in soil. Knowledge of P retention and release mechanisms when applying carbon-rich amendments such as biochar to soil is needed. The objectives of this study were to quantify the P sorption and availability from biochars produced from the fast pyrolysis of corn stover (Zea mays L.), Ponderosa pine (Pinus ponderosa Lawson and C. Lawson) wood residue, and switchgrass (Panicum virgatum L.). We determined the impact of biochar application to soils with different chemical characteristics on P sorption and availability. Sorption of P by biochars and soil-biochar mixtures was studied by fitting the equilibrium solution and sorbed concentrations of P using Freundlich and Langmuir isotherms. Biochar produced from Ponderosa pine wood residue had very different chemical characteristics than corn stover and switchgrass. Corn stover biochar had the highest P sorption (in average 79% of the initial solution P concentration) followed by switchgrass biochar (in average 76%) and Ponderosa pine wood residue biochar (in average 31%). Ponderosa pine wood residue biochar had higher bicarbonate extractable (available) P (in average 43%) followed by switchgrass biochar (33% of sorbed P) and corn stover biochar (25% of sorbed P). The incorporation of biochars to acidic soil at 40g/kg (4%) increased the equilibrium solution P concentration (reduced the sorption) and increased available sorbed P. In calcareous soil, application of alkaline biochars (corn stover and switchgrass biochars) significantly increased the sorption of P and decreased the availability of sorbed P. Biochar effects on soil P was aligned with their chemical composition and surface characteristics. C1 [Chintala, Rajesh; Schumacher, Thomas E.; Clay, David E.; Malo, Douglas D.; Clay, Sharon A.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [McDonald, Louis M.] W Virginia Univ, Dept Plant & Soil Sci, Morgantown, WV 26506 USA. [Papiernik, Sharon K.] ARS, USDA, Brookings, SD USA. [Julson, James L.] S Dakota State Univ, Dept Agr & Biosyst Engn, Brookings, SD 57007 USA. RP Chintala, R (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM rajesh.chintala@sdstate.edu FU Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture [2011-67009-30076] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67009-30076 from the USDA National Institute of Food and Agriculture. NR 58 TC 34 Z9 44 U1 14 U2 121 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-0650 EI 1863-0669 J9 CLEAN-SOIL AIR WATER JI Clean-Soil Air Water PD MAY PY 2014 VL 42 IS 5 BP 626 EP 634 DI 10.1002/clen.201300089 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Marine & Freshwater Biology; Water Resources SC Science & Technology - Other Topics; Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AJ2TD UT WOS:000337514900012 ER PT J AU Motta, D Langendoen, EJ Abad, JD Garcia, MH AF Motta, D. Langendoen, E. J. Abad, J. D. Garcia, M. H. TI Modification of meander migration by bank failures SO JOURNAL OF GEOPHYSICAL RESEARCH-EARTH SURFACE LA English DT Article DE bank erosion; slump block protection; floodplain soil heterogeneity; meander migration; meander planform shape; computer modeling ID PIPING SAPPING EROSION; LARGE WOODY DEBRIS; STREAMBANK EROSION; RIVER MEANDERS; BEND THEORY; VEGETATION; STABILITY; CHANNELS; MODEL; ERODIBILITY AB Meander migration and planform evolution depend on the resistance to erosion of the floodplain materials. To date, research to quantify meandering river adjustment has largely focused on resistance to erosion properties that vary horizontally. This paper evaluates the combined effect of horizontal and vertical floodplain material heterogeneity on meander migration by simulating fluvial erosion and cantilever and planar bank mass failure processes responsible for bank retreat. The impact of stream bank failures on meander migration is conceptualized in our RVR Meander model through a bank armoring factor associated with the dynamics of slump blocks produced by cantilever and planar failures. Simulation periods smaller than the time to cutoff are considered, such that all planform complexity is caused by bank erosion processes and floodplain heterogeneity and not by cutoff dynamics. Cantilever failure continuously affects meander migration, because it is primarily controlled by the fluvial erosion at the bank toe. Hence, it impacts migration rates and meander shapes through the horizontal and vertical distribution of erodibility of floodplain materials. Planar failures are more episodic. However, in floodplain areas characterized by less cohesive materials, they can affect meander evolution in a sustained way and produce preferential migration patterns. Model results show that besides the hydrodynamics, bed morphology and horizontal floodplain heterogeneity, floodplain stratigraphy can significantly affect meander evolution, both in terms of migration rates and planform shapes. Specifically, downstream meander migration can either increase or decrease with respect to the case of a homogeneous floodplain; lateral migration generally decreases as result of bank protection due to slump blocks; and the effect on bend skewness depends on the location and volumes of failed bank material caused by cantilever and planar failures along the bends, with possible achievement of downstream bend skewness under certain conditions. C1 [Motta, D.; Garcia, M. H.] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. [Langendoen, E. J.] ARS, Natl Sedimentat Lab, USDA, Oxford, MS USA. [Abad, J. D.] Univ Pittsburgh, Dept Civil & Environm Engn, Pittsburgh, PA USA. RP Motta, D (reprint author), Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. EM dmotta2@illinois.edu OI Langendoen, Eddy/0000-0002-2215-4989 FU U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station; Bureau of Land Management through the sale of public lands by the Southern Nevada Public Land Management Act; Department of Civil and Environmental Engineering at the University of Illinois at Urbana-Champaign [58-6408-8-265]; U.S. Department of Agriculture, Agricultural Research Service, National Sedimentation Laboratory [58-6408-8-265]; Department of Civil and Environmental Engineering of the University of Pittsburgh FX This research was supported by an agreement from the U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station and using funds provided by the Bureau of Land Management through the sale of public lands as authorized by the Southern Nevada Public Land Management Act. This work was performed under Specific Cooperative Agreement 58-6408-8-265 between the Department of Civil and Environmental Engineering at the University of Illinois at Urbana-Champaign and the U.S. Department of Agriculture, Agricultural Research Service, National Sedimentation Laboratory. Jorge D. Abad's participation was supported by his academic start-up funding provided by the Department of Civil and Environmental Engineering of the University of Pittsburgh. David Waterman is also acknowledged for the interesting discussions on bank erosion processes. NR 55 TC 5 Z9 5 U1 4 U2 30 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-9003 EI 2169-9011 J9 J GEOPHYS RES-EARTH JI J. Geophys. Res.-Earth Surf. PD MAY PY 2014 VL 119 IS 5 BP 1026 EP 1042 DI 10.1002/2013JF002952 PG 17 WC Geosciences, Multidisciplinary SC Geology GA AJ4DH UT WOS:000337620600004 ER PT J AU Neven, LG Lehrman, NJ Hansen, LD AF Neven, Lisa G. Lehrman, Nathan J. Hansen, Lee D. TI Effects of temperature and modified atmospheres on diapausing 5th instar codling moth metabolism SO JOURNAL OF THERMAL BIOLOGY LA English DT Article DE Codling moth; Cydia pomonella; Diapausing 5th instar; Metabolism; Differential scanning calorimetry; Modified atmospheres; Oxygen limitation; CO2 anesthesia ID PLATYNOTA-STULTANA PUPAE; OXYGEN LIMITATION; QUARANTINE TREATMENT; THERMAL TOLERANCE; LIFE STAGES; LEPIDOPTERA; TORTRICIDAE; INSECT; MORTALITY; WALNUTS AB The oxygen and Capacity limitation of thermal tolerance (OCLTT) has been established in aquatic insect larvae, but OCLTT has not been shown to generally apply to terrestrial insects. Previous research indicates that heat treatments in Combination with high Concentrations of Carbon dioxide and low Concentrations of oxygen may be effective for Controlling diapausing Codling moth, a quarantine pest in walnuts, but treatment requires long times and the killing mechanism is unknown. In this study, the effects of temperature and modified atmospheres on metabolism in diapausing 5th instar Codling moth (Cydia pomonella) was investigated with multi-channel differential scanning Calorimeters, one equipped with an oxygen sensor. O-2 Consumption and metabolic heat rates in air were measured simultaneously at isothermal temperatures from 5 to 50 degrees C at 5 degrees C intervals. Both rates increased with increasing temperatures from 5 to 40 degrees C. The ratio of metabolic heat rate to O-2 Consumption rate at temperatures <= 40 degrees C shows that a portion of the metabolic heat is from normal anabolic reactions of metabolism. At 45 and 50 degrees C in air, O-2 Consumption and metabolic heat rates dropped to near zero. These results indicate that treatment of walnuts in air at >45 degrees C for a short period of time (minutes) is effective in killing diapausing 5th instar Codling moth larvae.degrees Continuous heating scans at 0.4 degrees C/min were used to measure metabolic heat rates from 10 to 50 degrees C with air and modified atmospheres with lowered oxygen and high degrees Carbon dioxide. A rapid increase was observed in heat rates above 40 degrees C in scans with 02 >= 11%. Taken together with the isothermal results showing no metabolic heat production or oxygen uptake at 45 and 50 degrees C, these results demonstrate that thermal damage to Cell membranes and loss of Control of oxidation reactions is the lethal mechanism at high temperature when O-2 >= 11%. The data from scans with 02 <= 2% and high CO2 show the effects of oxygen limitation as postulated by the OCLTT. However,CO2 anesthesia appears to protect larvae from oxygen limitation at high temperature. These results show that treatment of walnuts in air at temperatures >45 degrees C will rapidly kill diapausing 5th instar Codling moths. Published by Elsevier Ltd. C1 [Neven, Lisa G.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Lehrman, Nathan J.] Cent Washington Univ, Dept Biol Sci, Ellensburg, WA 98926 USA. [Hansen, Lee D.] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA. RP Neven, LG (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM lisa.neven@ars.usda.gov NR 29 TC 13 Z9 13 U1 2 U2 41 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4565 J9 J THERM BIOL JI J. Therm. Biol. PD MAY PY 2014 VL 42 BP 9 EP 14 DI 10.1016/j.jtherbio.2014.02.017 PG 6 WC Biology; Zoology SC Life Sciences & Biomedicine - Other Topics; Zoology GA AJ4PU UT WOS:000337659100002 PM 24802143 ER PT J AU Ose, GA Hogsette, JA AF Ose, Gregory A. Hogsette, Jerome A. TI Spatial Distribution, Seasonality and Trap Preference of Stable Fly, Stomoxys Calcitrans L. (Diptera: Muscidae), Adults on a 12-Hectare Zoological Park SO ZOO BIOLOGY LA English DT Article DE alsynite fiberglass; sticky traps; UV light; cloth target; cylinder traps ID FLIES DIPTERA; BITING FLIES; ABUNDANCE; FLORIDA; POPULATIONS; CATTLE; DAIRY; NZI AB Although this study was originally designed to compare the efficacy of two different stable fly traps within 10 sites at a 12-ha zoological park, seasonal and spatial population distribution data were simultaneously collected. The two traps included an Alsynite fiberglass cylindrical trap (AFT) and a blue-black cloth target modified into a cylindrical trap (BCT). Both traps were covered with sticky sleeves to retain the attracted flies. Paired trap types were placed at sites that were 20-100 m apart. Distance between trap pairs within sites ranged from 1 to 2 m, and was limited by exhibit design and geography. Both trap types reflect/refract ultraviolet (UV) light which attracts adult S. calcitrans. During this 15-week study, AFTs captured significantly more stable flies than the BCTs at 8 of the 10 sites. Of the 12,557 stable flies found on the traps, 80% and 20% were captured by AFTs and BCTs, respectively. The most attractive trap site at the zoo was at the goat exhibit where most stable flies were consistently captured throughout the study. This exhibit was 100m from the other exhibits, next to a small lake, and adjacent to a field containing pastured exotic ungulates, rhea and ostrich. Stable fly populations peaked in early June then slowly decreased as the last trapping date approached. We believe this to be the first seasonality data collected at a zoological park. Results demonstrate the use of urban zoos by stable flies and the need to develop environmentally friendly stable fly management systems for zoos. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Ose, Gregory A.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Ose, Gregory A.] Smithsonian Natl Zool Pk, Ctr Anim Care Sci, Washington, DC USA. [Hogsette, Jerome A.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Hogsette, JA (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM jerry.hogsette@ars.usda.gov NR 30 TC 1 Z9 1 U1 1 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0733-3188 EI 1098-2361 J9 ZOO BIOL JI Zoo Biol. PD MAY-JUN PY 2014 VL 33 IS 3 BP 228 EP 233 DI 10.1002/zoo.21126 PG 6 WC Veterinary Sciences; Zoology SC Veterinary Sciences; Zoology GA AJ4XC UT WOS:000337681700009 PM 24740859 ER PT J AU Thomson, JL Tussing-Humphreys, LM Goodman, MH AF Thomson, Jessica L. Tussing-Humphreys, Lisa M. Goodman, Melissa H. TI Delta Healthy Sprouts: A randomized comparative effectiveness trial to promote maternal weight control and reduce childhood obesity in the Mississippi Delta SO CONTEMPORARY CLINICAL TRIALS LA English DT Article DE Maternal obesity; Gestational weight gain; Postnatal; Weight management; Childhood obesity ID LIFE-STYLE INTERVENTION; BODY-MASS INDEX; GESTATIONAL DIABETES-MELLITUS; INFANT-FEEDING PRACTICES; LOW-INCOME WOMEN; PREGNANT-WOMEN; DIETARY-INTAKE; RISK-FACTORS; GAIN; METAANALYSIS AB Introduction: Excessive and inadequate gestational weight gain can complicate a woman's pregnancy and put her and her child at risk for poor delivery and birth outcomes. Further, feeding and activity habits established early in life can significantly impact the development of childhood obesity. Methods: The on-going Delta Healthy Sprouts Project is a randomized, controlled, comparative trial testing the efficacy of two Maternal, Infant, and Early Childhood Home Visiting programs on weight status and health behaviors of 150 mothers and their infants residing in the rural Mississippi Delta region of the United States. Women are enrolled in their second trimester of pregnancy and randomized to one of two treatment arms. The control arm curriculum is based on Parents as Teachers, an evidence based approach to increase parental knowledge of child development and improve parenting practices. The experimental arm, labeled Parents as Teachers Enhanced, builds upon the control curriculum by including culturally tailored nutrition and physical activity components specifically designed for the gestational and postnatal periods. We hypothesize that, as compared to the control arm, the experimental arm will be more effective in preventing inappropriate gestational weight gain, reducing postnatal weight retention, and decreasing infant obesity rates. We also will evaluate mother and child dietary and physical activity outcomes, breastfeeding initiation and continuation, and child feeding practices. Conclusion: The Delta Healthy Sprouts Project tests a novel, combined approach to maternal weight management and childhood obesity prevention in pregnant women and their children at high risk for obesity and chronic disease. Published by Elsevier Inc. C1 [Thomson, Jessica L.; Goodman, Melissa H.] USDA ARS, Stoneville, MS 38776 USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Dept Med, Chicago, IL 60608 USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Ctr Canc, Chicago, IL 60608 USA. RP Thomson, JL (reprint author), USDA ARS, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM jessica.thomson@ars.usda.gov; ltussing@uic.edu; melissa.goodman@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service Project [6401-53000-001-00D]; Delta Health Alliance FX We thank Candace Feazell, Tracy Grady, and Melinda Young for their research support and for reviewing an earlier version of this manuscript. This research is supported by the U.S. Department of Agriculture, Agricultural Research Service Project 6401-53000-001-00D, and in kind support from the Delta Health Alliance. The views expressed are solely those of the authors and do not reflect the official policy or position of the US government. NR 89 TC 3 Z9 3 U1 6 U2 30 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1551-7144 EI 1559-2030 J9 CONTEMP CLIN TRIALS JI Contemp. Clin. Trials PD MAY PY 2014 VL 38 IS 1 BP 82 EP 91 DI 10.1016/j.cct.2014.03.004 PG 10 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA AI8VA UT WOS:000337203900009 PM 24685997 ER PT J AU Schlaepfer, DR Ewers, BE Shuman, BN Williams, DG Frank, JM Massman, WJ Lauenroth, WK AF Schlaepfer, Daniel R. Ewers, Brent E. Shuman, Bryan N. Williams, David G. Frank, John M. Massman, William J. Lauenroth, William K. TI Terrestrial water fluxes dominated by transpiration: Comment SO ECOSPHERE LA English DT Editorial Material ID ISOTOPIC COMPOSITIONS; MOUNTAIN CATCHMENT; EDDY COVARIANCE; CLIMATE-CHANGE; UNITED-STATES; SNOW; SUBLIMATION; EVAPOTRANSPIRATION; COMPONENTS; HYDROGEN C1 [Schlaepfer, Daniel R.; Ewers, Brent E.; Williams, David G.; Frank, John M.; Lauenroth, William K.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Ewers, Brent E.; Shuman, Bryan N.; Williams, David G.; Frank, John M.; Lauenroth, William K.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Shuman, Bryan N.] Univ Wyoming, Dept Geol & Geophys, Laramie, WY 82071 USA. [Williams, David G.] Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. [Frank, John M.; Massman, William J.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Schlaepfer, DR (reprint author), Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. EM dschlaep@uwyo.edu RI Williams, David/A-6407-2014; Schlaepfer, Daniel/D-1756-2009 OI Williams, David/0000-0003-3627-5260; Schlaepfer, Daniel/0000-0001-9973-2065 NR 47 TC 12 Z9 12 U1 9 U2 47 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2014 VL 5 IS 5 AR 61 DI 10.1890/ES13-00391.1 PG 9 WC Ecology SC Environmental Sciences & Ecology GA AI8KW UT WOS:000337164100012 ER PT J AU Vaid, TM Runkle, ES Frantz, JM AF Vaid, Tasneem M. Runkle, Erik S. Frantz, Jonathan M. TI Mean Daily Temperature Regulates Plant Quality Attributes of Annual Ornamental Plants SO HORTSCIENCE LA English DT Article DE bedding plants; floriculture crops; flowering; greenhouse crops; scheduling ID ENRICHMENT AFFECT GROWTH; CARPATICA BLUE CLIPS; FLOWER DEVELOPMENT; NIGHT TEMPERATURES; LIGHT; IRRADIANCE; PHOTOPERIOD; MORPHOLOGY; EXPOSURE; XHYBRIDA AB In protected environments, temperature is often regulated to produce ornamental crops for specific market dates. Temperature primarily controls plant developmental rate and thus production time, but it can also interact with light quantity to affect crop quality attributes such as flower number, branching, and biomass accumulation. We quantified how mean daily temperature (MDT) between 14 and 26 degrees C influenced quality characteristics of 15 common bedding plant crops. American marigold (Tagetes erecta), cup flower (Nierembergia caerulea), diascia (Diascia barberae), flowering tobacco (Nicotiana alata), geranium (Pelargonium x hortorum), globe amaranth (Gomphrena globosa), heliotrope (Heliotropium arborescens), nemesia (Nemesia foetans), New Guinea impatiens (Impatiens hawkeri), osteospermum (Osteospermum ecklonis), pot marigold (Calendula officinalis), snapdragon (Antirrhinum majus), stock (Matthiola incana), and torenia (Torenia fournieri) were grown under two mean daily light integrals (9.0 and 18.0 mol.m(-2).d(-1)) in five environmentally controlled greenhouse compartments with a 16-h photoperiod. As MDT increased from 14 to 26 degrees C, flower or inflorescence number decreased for nearly all crops. In six crops, flower or inflorescence size decreased as MDT increased, whereas in five crops, there was an initial increase in flower size with an increase in MDT and then a subsequent decrease at MDT greater than 20 degrees C. In 10 of the crops, shoot weight at flowering decreased linearly or quadratically with an increase in MDT. Branch number was inversely related with MDT in eight crops and was positively correlated with an increase in flower number. We conclude that in a majority of the crops studied, plant quality decreased as the MDT increased, which can at least partially be attributed to earlier flowering at the higher MDTs. Therefore, there is often a tradeoff between faster crop timing and higher plant quality, especially for plants with a low estimated base temperature (T-min) for development. C1 [Vaid, Tasneem M.; Runkle, Erik S.] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Frantz, Jonathan M.] ARS, USDA, ATRU, Toledo, OH 43606 USA. RP Runkle, ES (reprint author), Michigan State Univ, Dept Hort, 1066 Bogue St,Room A288, E Lansing, MI 48824 USA. EM runkleer@msu.edu FU USDA-ARS Floriculture and Nursery Research Initiative; Michigan State University FX We gratefully acknowledge funding and support by the USDA-ARS Floriculture and Nursery Research Initiative, Michigan State University's Project GREEEN and AgBioResearch, and horticulture companies providing support for Michigan State University floriculture research. We also thank C. Raker & Sons for donation of plant material and Mike Olrich for greenhouse assistance. NR 32 TC 3 Z9 4 U1 3 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2014 VL 49 IS 5 BP 574 EP 580 PG 7 WC Horticulture SC Agriculture GA AI8WW UT WOS:000337208700009 ER PT J AU Strik, BC Buller, G Tarara, JM AF Strik, Bernadine C. Buller, Gil Tarara, Julie M. TI Grow Tubes Reduce Root and Crown Growth but Not Early Production during Establishment of Highbush Blueberry SO HORTSCIENCE LA English DT Article DE dry weight partitioning; early cropping; pruning; root-to-shoot ratio; tree shelters; Vaccinium corymbosum; vine shelters ID ORGANIC PRODUCTION; TREE SHELTERS; GAS-EXCHANGE; TREESHELTERS; ALLOCATION; SEEDLINGS; BIOMASS; BLACKBERRY; IMPACT; YIELD AB Grow tubes are sometimes used in blueberry (Vaccinium corymbosum L.) to establish plantings or replace dead plants in older fields. Two experiments were conducted at a commercial farm to evaluate the effect of various grow tubes used during planting establishment of highbush blueberry cultivars. The treatments in the first experiment were cultivar ('Aurora', 'Elliott', 'Liberty') and grow tube treatment (no tube, control; opaque cardboard tube in the first growing season; and opaque plastic tube in the first season or first through the second season). The treatments in the second experiment were cultivar ('Aurora', 'Elliott', 'Liberty', 'Ozarkblue') and grow tube treatment (control; translucent plastic; opaque plastic; and wire mesh tube over plants in the first growing season). The presence of a grow tube from spring to fall of the first growing season decreased crown dry weight (DW) by an average of 37% to 50% and root DW by 30% (all except translucent plastic in Expt. 2) and increased the aboveground:belowground DW ratio relative to the control by an average of 34% to 67%, depending on the experiment. Plants grown in tubes were taller, had a narrower canopy, and had fewer whips, likely a response to low light levels inside the tubes; the fewest whips were found in the opaque plastic or cardboard tubes and the most in the translucent plastic tube with an intermediate response in the wire mesh tube. Removal of grow tubes during the summer led to plant damage from sudden sun exposure. The opaque grow tubes (present in Year 1) reduced yield/plant in Year 2 for 'Elliott' and 'Liberty' (cardboard tube only) but not 'Aurora'. Pruning plants to allow for limited early fruit production (approximate to 0.6 kg/plant) in Year 2 did not reduce yield in Year 3 (approximate to 2.7 kg/plant). Whereas grow tubes reduced root and crown growth in the first season, there appeared to be no longer-term adverse effect on aboveground plant growth or yield. C1 [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Buller, Gil] North Willamette Res & Extens Ctr, Aurora, OR 97002 USA. [Tarara, Julie M.] USDA ARS, Hort Crops Res Unit, Prosser, WA 99350 USA. RP Strik, BC (reprint author), Oregon State Univ, Dept Hort, 4017 ALS, Corvallis, OR 97331 USA. EM bernadine.strik@oregonstate.edu FU Oregon Blueberry Commission FX We appreciate the research support provided by the Oregon Blueberry Commission and the valuable contributions of our grower collaborator (PanAmerican Berry Growers, Salem, OR). NR 52 TC 1 Z9 1 U1 2 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2014 VL 49 IS 5 BP 581 EP 588 PG 8 WC Horticulture SC Agriculture GA AI8WW UT WOS:000337208700010 ER PT J AU Tarara, JM Chaves, B Strik, BC AF Tarara, Julie M. Chaves, Bernardo Strik, Bernadine C. TI Grow Tubes Change Microclimate and Bush Architecture but Have Little Effect on Bush Biomass Allocation at the End of the Establishment Year in Blueberry SO HORTSCIENCE LA English DT Article DE mulch; radiation; temperature; tree shelter; Vaccinium corymbosum ID GAS-EXCHANGE CHARACTERISTICS; HIGHBUSH BLUEBERRY; FRUIT-QUALITY; LIGHT ENVIRONMENT; L. SEEDLINGS; TREESHELTERS; SHELTERS; MULCH; PHOTOSYNTHESIS; MANAGEMENT AB Microclimate variables were integrated over a 6-month period during which blueberry (Vaccinium corymbosum cv. Liberty) bushes were grown in 51-cm high, 20-cm diameter round grow tubes (opaque or translucent) on a sawdust mulch-covered raised bed with the mulch incorporated into tilled soil. Grow tubes were installed around plants in the spring of 2006, 5 months after planting. Total photosynthetic photon flux (PPF) density was 55% and 21% of ambient in translucent and opaque tubes, respectively. Daily maximum vapor pressure deficit consistently was highest in translucent tubes. Air (T-a) and stem (T-stem) temperatures in both grow tube types exceeded T-a and T-stem in non-tubed plants (ambient). Maximum mulch surface temperature (T-m) was lowest in opaque tubes, whereas there was no difference in T-m between ambient and translucent tubes. The soil mulch interface temperature (T-sm) was warmer outside tubes than T-sm inside tubes. Soil temperatures directly under the tubes differed very little between tube types and ambient, generally less than 1 degrees C. Root and crown dry mass (DM) did not differ between tubed plants and ambient at the end of the establishment year. Leaf area, leaf DM, and fruit bud number were suppressed inside tubes. All plants were greater than 51 cm tall at the end of the growing season. Substantial compensatory growth occurred above tubes: tubed plants were more upright and had more leaf area, leaf DM, and shoot growth than ambient plants above 51 cm. However, there was no difference between tubed and ambient plants in fruit bud number, total plant leaf area, shoot:root, or DM of 1- and 2-year-old wood. Grow tubes can alter microclimate and architecture of young blueberry bushes but have no significant influence on size and distribution of total DM after one growing season in the field. C1 [Tarara, Julie M.] USDA ARS, Hort Crops Res Unit, Prosser, WA 99350 USA. [Chaves, Bernardo] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97330 USA. RP Tarara, JM (reprint author), USDA ARS, Hort Crops Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM julie.tarara@ars.usda.gov FU Oregon Blueberry Commission FX We appreciate the research support provided by the Oregon Blueberry Commission and the valuable contributions of our grower collaborator (PanAmerican Berry Growers, Salem, OR). NR 50 TC 2 Z9 2 U1 1 U2 9 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2014 VL 49 IS 5 BP 596 EP 602 PG 7 WC Horticulture SC Agriculture GA AI8WW UT WOS:000337208700012 ER PT J AU Dumroese, RK Williams, JL Pinto, JR Zhang, P AF Dumroese, R. Kasten Williams, Jasmine L. Pinto, Jeremiah R. Zhang, Peng TI Oxyfluorfen Strongly Affects Larix occidentalis but Minimally Affects Sagina procumbens in a Bareroot Nursery SO HORTSCIENCE LA English DT Article DE biomass; canopy cover; Larix occidentalis; postemergence; preemergence; Sagina procumbens; seedling emergence ID SEEDLINGS; PINE AB Our objective was to evaluate oxyfluorfen for control of birdseye pearlwort (Sagina procumbens L.) in a bareroot nursery crop of western larch (Larix occidentalis Nutt.) seedlings. Oxyfluorfen applied at rates up to 0.56 kg a.i./ha in a split-plot experiment with combinations and frequencies of pre- and postemergence sprays gave minimal control of birdseye pearlwort. Although preemergence rates 0.42 kg a.i./ha or greater reduced western larch emergence 10% compared with the control, final seedling inventory was similar for rates 0.42 kg a.i./ha or less. Seedlings receiving 0.42 kg a.i./ha or greater grew 30% more biomass than those that received 0.28 kg a.i./ha or less. When applied postemergence, oxyfluorfen reduced the number of larch seedlings at final inventory 9% and those seedlings had 20% less biomass than the control. Oxyfluorfen applied preemergence increased the amount of bare soil (reduced the weed canopy) throughout the production cycle compared with the control but even the most efficacious treatment combinations still had birdseye pearlwort canopy coverage 63% or greater. C1 [Dumroese, R. Kasten; Pinto, Jeremiah R.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Williams, Jasmine L.] US Forest Serv, USDA, Idaho Panhandle Natl Forest, Coeur dAlene Nursery, Coeur Dalene, ID 83815 USA. [Zhang, Peng] Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China. RP Dumroese, RK (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. EM kdumroese@fs.fed.us FU U.S. Department of Agriculture, Forest Service, National Center for Reforestation, Nursery, and Genetic Resources; Coeur d'Alene Nursery (Region 1); Rocky Mountain Research Station FX This work was funded by the U.S. Department of Agriculture, Forest Service, National Center for Reforestation, Nursery, and Genetic Resources, the Coeur d'Alene Nursery (Region 1), and the Rocky Mountain Research Station. NR 28 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2014 VL 49 IS 5 BP 603 EP 607 PG 5 WC Horticulture SC Agriculture GA AI8WW UT WOS:000337208700013 ER PT J AU Simko, I Hayes, RJ Bull, CT Mou, BQ Luo, YG Trent, MA Atallah, AJ Ryder, EJ Sideman, RG AF Simko, Ivan Hayes, Ryan J. Bull, Carolee T. Mou, Beiquan Luo, Yaguang Trent, Mark A. Atallah, Amy J. Ryder, Edward J. Sideman, Rebecca G. TI Characterization and Performance of 16 New Inbred Lines of Lettuce SO HORTSCIENCE LA English DT Editorial Material DE breeding; disease resistance; genetics; Lactuca sativa; postharvest quality; production; vegetable market ID CAMPESTRIS PV. VITIANS; LACTUCA-SATIVA L.; VERTICILLIUM WILT; DOWNY MILDEW; RESISTANCE; QUALITY C1 [Simko, Ivan; Hayes, Ryan J.; Bull, Carolee T.; Mou, Beiquan; Trent, Mark A.; Atallah, Amy J.; Ryder, Edward J.; Sideman, Rebecca G.] ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. [Luo, Yaguang] ARS, USDA, Food Qual Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Simko, I (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM Ivan.Simko@ars.usda.gov RI Simko, Ivan/J-6048-2012 OI Simko, Ivan/0000-0002-8769-8477 NR 32 TC 6 Z9 6 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2014 VL 49 IS 5 BP 679 EP 687 PG 9 WC Horticulture SC Agriculture GA AI8WW UT WOS:000337208700027 ER PT J AU Lamar, RT Olk, DC Mayhew, L Bloom, PR AF Lamar, Richard T. Olk, Daniel C. Mayhew, Lawrence Bloom, Paul R. TI A New Standardized Method for Quantification of Humic and Fulvic Acids in Humic Ores and Commercial Products SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID FRACTIONATION; SUBSTANCES AB Increased use of humic substances in agriculture has generated intense interest among producers, consumers, and regulators for an accurate and reliable method to quantify humic acid (HA) and fulvic acid (FA) in raw ores and products. Here we present a thoroughly validated method, the new standardized method for determination of HA and FA contents in raw humate ores and in solid and liquid products produced from them. The methods used for preparation of HA and FA were adapted according to the guidelines of the International Humic Substances Society involving alkaline extraction followed by acidification to separate HA from the fulvic fraction. This is followed by separation of FA from the fulvic fraction by adsorption on a nonionic macroporous acrylic ester resin at acid pH. It differs from previous methods in that it determines HA and FA concentrations gravimetrically on an ash-free basis. Critical steps in the method, e.g., initial test portion mass, test portion to extract volume ratio, extraction time, and acidification of alkaline extract, were optimized for maximum and consistent recovery of HA and FA. The method detection limits for HA and FA were 4.62 and 4.8 mg/L, respectively. The method quantitation limits for HA and FA were 14.7 and 15.3 mg/L, respectively. C1 [Lamar, Richard T.] Horizon Ag Prod, Louisville, CO 80037 USA. [Olk, Daniel C.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Mayhew, Lawrence] EAM Consulting, Spring Green, WI 53588 USA. [Bloom, Paul R.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Lamar, RT (reprint author), Horizon Ag Prod, 1450 Infinite Dr, Louisville, CO 80037 USA. EM rlamar@horizonag.com FU Humic Products Trade Association FX This study was entirely funded by the Humic Products Trade Association. We gratefully appreciate the assistance and reference materials donated by the IHSS, and gratefully acknowledge the valuable assistance provided by James Bartos, Office of Indiana State Chemist, the AAPFCO, and the SLV study monitors: Elaine Wong (CDFA), John Peterson (Wilber Ellis), Nancy Thiex (South Dakota State University), and Debra Wong (Oregon Department of Agriculture). NR 18 TC 2 Z9 2 U1 7 U2 45 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAY-JUN PY 2014 VL 97 IS 3 BP 721 EP 730 DI 10.5740/jaoacint.13-393 PG 10 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA AI8ZT UT WOS:000337216200008 PM 25051616 ER PT J AU Tooley, PW Browning, M Leighty, RM AF Tooley, Paul W. Browning, Marsha Leighty, Robert M. TI The effect of temperature on germination of chlamydospores of Phytophthora ramorum SO MYCOLOGIA LA English DT Article DE overwintering; ramorum blight; resting spore; sudden oak death ID SUDDEN OAK DEATH; PATHOGEN; SOIL; MANAGEMENT; CALIFORNIA; CINNAMOMI; PALMIVORA; MEDIA AB Mycelium-free chlamydospores of 12 isolates of P. ramorum representing three clonal lineages were produced with a method involving incubation in nonsterile sand at 20 C in darkness for 30 d. Chlamydospores were incubated on selective agar medium at 5, 10, 15, 20, 25 and 30 C and germination assessed after 1, 2, 4, 6 and 8 d incubation. The optimal temperature for germination based on 8 d incubation was 20 C for all three clonal lineages tested (NA1 NA2, EU1). Mean germination rates were 2, 21, 44, 67, 32 and 0 percent at 5, 10, 15, 20, 25 and 30 C respectively for all isolates combined. The highest mean germination rate was scored by isolates of the EU1 clonal lineage at 20 C (85%) after 8 d incubation However, substantial variation was observed among isolates within each clonal lineage. Overall temperatures and days of incubation on which germination was assessed isolates of the NA1 clonal lineage had the lowest mean germination, even though one isolate had the highest germination of any isolate in any lineage. The results indicate that 20 C is the optimal germination temperature for P. ramorum chlamydospores and that a great disparity in germination percentage can exist within isolates, even within a single clonal lineage. C1 [Tooley, Paul W.; Browning, Marsha] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Leighty, Robert M.] Data Management Serv Inc, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA. RP Tooley, PW (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM paul.tooley@ars.usda.gov NR 30 TC 2 Z9 2 U1 0 U2 13 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2014 VL 106 IS 3 BP 424 EP 430 DI 10.3852/13-313 PG 7 WC Mycology SC Mycology GA AI7LF UT WOS:000337071400006 PM 24871605 ER PT J AU Abad, ZG Abad, JA Cacciola, SO Pane, A Faedda, R Moralejo, E Perez-Sierra, A Abad-Campos, P Alvarez-Bernaola, LA Bakonyi, J Jozsa, A Herrero, ML Burgess, TI Cunnington, JH Smith, IW Balci, Y Blomquist, C Henricot, B Denton, G Spies, C McLeod, A Belbahri, L Cooke, D Kageyama, K Uematsu, S Kurbetli, I Degirmenci, K AF Abad, Z. Gloria Abad, Jorge A. Cacciola, Santa Olga Pane, Antonella Faedda, Roberto Moralejo, Eduardo Perez-Sierra, Ana Abad-Campos, Paloma Alvarez-Bernaola, Luis A. Bakonyi, Jozsef Jozsa, Andras Herrero, Maria Luz Burgess, Treena I. Cunnington, James H. Smith, Ian W. Balci, Yilmaz Blomquist, Cheryl Henricot, Beatrice Denton, Geoffrey Spies, Chris McLeod, Adele Belbahri, Lassaad Cooke, David Kageyama, Koji Uematsu, Seiji Kurbetli, Ilker Degirmenci, Kemal TI Phytophthora niederhauserii sp nov., a polyphagous species associated with ornamentals, fruit trees and native plants in 13 countries SO MYCOLOGIA LA English DT Article DE beta-tub; EF-1 alpha; ITS; Oomycetes; plant pathogen; Straminipila; taxonomy ID PHYLOGENETIC ANALYSIS; TAXON NIEDERHAUSERII; MOLECULAR PHYLOGENY; WESTERN-AUSTRALIA; PATHOGEN; SEQUENCES; ROOT; ROT; BANKSIA; RAMORUM AB A non-papillate, heterothallic Phytophthora species first isolated in 2001 and subsequently from symptomatic roots, crowns and stems of 33 plant species in 25 unrelated botanical families from 13 countries is formally described here as a new species. Symptoms on various hosts included crown and stem rot, chlorosis, wilting, leaf blight, cankers and gumming. This species was isolated from Australia, Hungary, Israel, Italy, Japan, the Netherlands, Norway, South Africa, Spain, Taiwan, Turkey, the United Kingdom and United States in association with shrubs and herbaceous ornamentals grown mainly in greenhouses. The most prevalent hosts are English ivy (Hedera helix) and Cistus (Cistus salvifolius). The association of the species with acorn banksia (Banksia prionotes) plants in natural ecosystems in Australia, in affected vineyards (Vitis vinifera) in South Africa and almond (Prunus dulcis) trees in Spain and Turkey in addition to infection of shrubs and herbaceous ornamentals in a broad range of unrelated families are a sign of a wide ecological adaptation of the species and its potential threat to agricultural and natural ecosystems. The morphology of the persistent non-papillate ellipsoid sporangia, unique toruloid lobate hyphal swellings and amphigynous antheridia does not match any of the described species. Phylogenetic analysis based on sequences of the ITS rDNA, EF-1 alpha, and beta-tub supported that this organism is a hitherto unknown species. It is closely related to species in ITS clade 7b with the most closely related species being P. sojae. The name Phytophthora niederhauserii has been used in previous studies without the formal description of the holotype. This name is validated in this manuscript with the formal description of Phytophthora niederhauserii Z.G. Abad et J.A. Abad, sp. nov. The name is coined to honor Dr John S. Niederhauser, a notable plant pathologist and the 1990 World Food Prize laureate. C1 [Abad, Z. Gloria] USDA, USDA APHIS PPQ S&T CPHST, Ctr Plant Hlth Sci & Technol, BARG E, Beltsville, MD 20705 USA. [Abad, Jorge A.] USDA, USDA APHIS PPQ FO PGQP, Plant Germplasm Quarantine Program, BARG E, Beltsville, MD 20705 USA. [Cacciola, Santa Olga; Pane, Antonella; Faedda, Roberto] Univ Catania, Dept Agri Food & Environm Syst Management, Plant Pathol Sect, I-95123 Catania, Italy. [Moralejo, Eduardo] MEDEA CSIC UIB, Mycol Lab, Inst Mediterraneo Estudios Avanzados, Esporles 07190, Balearic Island, Spain. [Perez-Sierra, Ana; Abad-Campos, Paloma; Alvarez-Bernaola, Luis A.] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, Valencia 46022, Spain. [Bakonyi, Jozsef] Hungarian Acad Sci, Agr Res Ctr, Inst Plant Protect, H-1022 Budapest, Hungary. [Jozsa, Andras] Univ Pannonia, Georgikon Fac, Inst Plant Protect, H-8360 Keszthely, Hungary. [Herrero, Maria Luz] Norwegian Inst Agr & Environm Res, N-1432 As, Norway. [Burgess, Treena I.] Murdoch Univ, Ctr Phytophthora Sci & Management, Sch Biol Sci & Biotechnol, Murdoch, WA 6150, Australia. [Cunnington, James H.] Knoxfield Ctr, Dept Primary Ind, Ferntree Gully, Vic 3156, Australia. [Smith, Ian W.] Univ Melbourne, Dept Forest & Ecosyst Sci, Richmond, Vic 3121, Australia. [Balci, Yilmaz] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20740 USA. [Blomquist, Cheryl] Calif Dept Food & Agr, Plant Pests & Diagnost Branch, Sacramento, CA 95832 USA. [Henricot, Beatrice; Denton, Geoffrey] Royal Hort Soc Wisley, Dept Plant Pathol, Woking, Surrey GU23 6QB, England. [Spies, Chris] Agr & Agri Food Canada, Ottawa, ON K1A 0C6, Canada. [McLeod, Adele] Univ Stellenbosch, Dept Plant Pathol, ZA-7602 Matieland, South Africa. [Belbahri, Lassaad] Univ Neuchatel, Lab Soil Biol, CH-2000 Neuchatel, Switzerland. [Cooke, David] James Hutton Inst, Dundee, Scotland. [Kageyama, Koji] Gifu Univ, River Basin Res Ctr, Gifu 5011193, Japan. [Uematsu, Seiji] Chiba Prefectural Agr Res Ctr, Inst Hort, Tateyama, Toyama, Japan. [Kurbetli, Ilker; Degirmenci, Kemal] Plant Protect Cent Res Inst, Dept Phytopathol, Ankara, Turkey. RP Abad, ZG (reprint author), USDA, USDA APHIS PPQ S&T CPHST, Ctr Plant Hlth Sci & Technol, BARG E, Bldg 580,Powder Mill Rd, Beltsville, MD 20705 USA. EM Gloria.Abad@aphis.usda.gov RI McLeod, Adele/D-4025-2012; Cooke, David/D-1933-2012; Bakonyi, Jozsef/A-2318-2017; Burgess, Treena/G-4770-2011 OI McLeod, Adele/0000-0002-1807-9154; Cooke, David/0000-0002-9154-7954; Burgess, Treena/0000-0002-7962-219X FU Western Australian Department of Agriculture [VPRI 32086]; North Carolina Department of Agriculture and Consumer Services [PH2424]; Hungarian Scientific Research Fund (OTKA) [K61107, K101914]; Scottish government; Italian Ministry of University and Research (MIUR); Swiss State Secretariat for Education and Research [C09.0139]; European Union [245268]; COST action [FP0801] FX We thank Dr Michael Coffey at the World Oomycete Genetic Resource (WOC) and World Phytophthora Genetic Resource (WPC) Collections for providing valuable information for this manuscript. We also thank Dr Elaine Davison at Western Australian Department of Agriculture for providing isolate VPRI 32086 to James H. Cunnington. The contribution of Dr Suzanne Spencer at the North Carolina Department of Agriculture and Consumer Services providing isolate PH2424 to Z. Gloria Abad is appreciated. Research in Hungary was supported by the Hungarian Scientific Research Fund (OTKA) grants K61107 and K101914. Research in Scotland was support by the Scottish government. Research in Italy was supported by Italian Ministry of University and Research (MIUR; PRIN 2008). LB is supported by the Swiss State Secretariat for Education and Research (grant reference: SER No. C09.0139) and the European Union for the projects ISEFOR "Increasing sustainability of European forests: modeling for security against invasive pests and pathogens under climate change (FP7- KBBE-2009-3 call, proposal number 245268) and the COST action FP0801 "Established and emerging Phytophthora: increasing threats to woodland and forest ecosystems in Europe". NR 53 TC 10 Z9 11 U1 0 U2 27 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2014 VL 106 IS 3 BP 431 EP 447 DI 10.3852/12-119 PG 17 WC Mycology SC Mycology GA AI7LF UT WOS:000337071400007 PM 24871599 ER PT J AU Blackwell, M Rossman, A Palm, M Crous, PW AF Blackwell, Meredith Rossman, Amy Palm, Mary Crous, Pedro W. TI Emory Guy Simmons 1920-2013 SO MYCOLOGIA LA English DT Biographical-Item DE Alternaria; International Mycological Association; Mycological Society of America; QM 6a; Thailand C1 [Blackwell, Meredith] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Rossman, Amy] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. [Palm, Mary] USDA, Anim & Plant Hlth Inspect Serv, Natl Identificat Serv, Riverdale, MD USA. [Crous, Pedro W.] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. RP Blackwell, M (reprint author), Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. EM mblackwell@lsu.edu RI Crous, Pedro/H-1489-2012 OI Crous, Pedro/0000-0001-9085-8825 NR 4 TC 0 Z9 0 U1 1 U2 1 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 EI 1557-2536 J9 MYCOLOGIA JI Mycologia PD MAY-JUN PY 2014 VL 106 IS 3 BP 610 EP 614 DI 10.3852/13-232 PG 5 WC Mycology SC Mycology GA AI7LF UT WOS:000337071400022 PM 25121187 ER PT J AU Terrapon, N Li, C Robertson, HM Ji, L Meng, XH Booth, W Chen, ZS Childers, CP Glastad, KM Gokhale, K Gowin, J Gronenberg, W Hermansen, RA Hu, HF Hunt, BG Huylmans, AK Khalil, SMS Mitchell, RD Munoz-Torres, MC Mustard, JA Pan, HL Reese, JT Scharf, ME Sun, FM Vogel, H Xiao, J Yang, W Yang, ZK Yang, ZQ Zhou, JJ Zhu, JW Brent, CS Elsik, CG Goodisman, MAD Liberles, DA Roe, RM Vargo, EL Vilcinskas, A Wang, J Bornberg-Bauer, E Korb, J Zhang, GJ Liebig, J AF Terrapon, Nicolas Li, Cai Robertson, Hugh M. Ji, Lu Meng, Xuehong Booth, Warren Chen, Zhensheng Childers, Christopher P. Glastad, Karl M. Gokhale, Kaustubh Gowin, Johannes Gronenberg, Wulfila Hermansen, Russell A. Hu, Haofu Hunt, Brendan G. Huylmans, Ann Kathrin Khalil, Sayed M. S. Mitchell, Robert D. Munoz-Torres, Monica C. Mustard, Julie A. Pan, Hailin Reese, Justin T. Scharf, Michael E. Sun, Fengming Vogel, Heiko Xiao, Jin Yang, Wei Yang, Zhikai Yang, Zuoquan Zhou, Jiajian Zhu, Jiwei Brent, Colin S. Elsik, Christine G. Goodisman, Michael A. D. Liberles, David A. Roe, R. Michael Vargo, Edward L. Vilcinskas, Andreas Wang, Jun Bornberg-Bauer, Erich Korb, Judith Zhang, Guojie Liebig, Jurgen TI Molecular traces of alternative social organization in a termite genome SO NATURE COMMUNICATIONS LA English DT Article ID DNA METHYLATION; ZOOTERMOPSIS-ANGUSTICOLLIS; RETICULITERMES-FLAVIPES; PROTEASOME PATHWAY; SIAH-1 INTERACTS; GENE-EXPRESSION; APIS-MELLIFERA; DRAFT GENOME; HONEY-BEE; EVOLUTION AB Although eusociality evolved independently within several orders of insects, research into the molecular underpinnings of the transition towards social complexity has been confined primarily to Hymenoptera (for example, ants and bees). Here we sequence the genome and stage-specific transcriptomes of the dampwood termite Zootermopsis nevadensis (Blattodea) and compare them with similar data for eusocial Hymenoptera, to better identify commonalities and differences in achieving this significant transition. We show an expansion of genes related to male fertility, with upregulated gene expression in male reproductive individuals reflecting the profound differences in mating biology relative to the Hymenoptera. For several chemoreceptor families, we show divergent numbers of genes, which may correspond to the more claustral lifestyle of these termites. We also show similarities in the number and expression of genes related to caste determination mechanisms. Finally, patterns of DNA methylation and alternative splicing support a hypothesized epigenetic regulation of caste differentiation. C1 [Terrapon, Nicolas; Huylmans, Ann Kathrin; Bornberg-Bauer, Erich] Univ Munster, Inst Evolut & Biodivers, D-48149 Munster, Germany. [Li, Cai; Ji, Lu; Meng, Xuehong; Chen, Zhensheng; Hu, Haofu; Pan, Hailin; Sun, Fengming; Xiao, Jin; Yang, Wei; Yang, Zhikai; Yang, Zuoquan; Zhou, Jiajian; Wang, Jun; Zhang, Guojie] BGI Shenzhen, China Natl GeneBank, Shenzhen, Peoples R China. [Li, Cai] Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, DK-1350 Copenhagen, Denmark. [Robertson, Hugh M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Booth, Warren; Khalil, Sayed M. S.; Mitchell, Robert D.; Zhu, Jiwei; Roe, R. Michael; Vargo, Edward L.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Booth, Warren; Khalil, Sayed M. S.; Mitchell, Robert D.; Zhu, Jiwei; Roe, R. Michael; Vargo, Edward L.] N Carolina State Univ, WM Keck Ctr Behav Biol, Raleigh, NC 27695 USA. [Childers, Christopher P.; Reese, Justin T.; Elsik, Christine G.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Glastad, Karl M.; Hunt, Brendan G.; Goodisman, Michael A. D.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. [Gokhale, Kaustubh; Mustard, Julie A.; Liebig, Jurgen] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Gowin, Johannes; Korb, Judith] Univ Osnabruck, D-49076 Osnabruck, Germany. [Gronenberg, Wulfila] Univ Arizona, Dept Neurosci, Tucson, AZ 85721 USA. [Hermansen, Russell A.; Liberles, David A.] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA. [Khalil, Sayed M. S.] Agr Genet Engn Res Inst, Dept Microbial Mol Biol, Giza 12619, Egypt. [Munoz-Torres, Monica C.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Genom Div, Berkeley, CA 94720 USA. [Scharf, Michael E.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Vogel, Heiko] Max Planck Inst Chem Ecol, Dept Entomol, D-07745 Jena, Germany. [Brent, Colin S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Elsik, Christine G.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Vilcinskas, Andreas] Univ Giessen, Inst Phytopathol & Angew, D-35390 Giessen, Germany. [Wang, Jun] Univ Copenhagen, Dept Biol, DK-1165 Copenhagen, Denmark. [Wang, Jun] King Abdulaziz Univ, Princess Jawhara Ctr Excellence Res Hereditary Di, Jeddah 21589, Saudi Arabia. [Wang, Jun] Macau Univ Sci & Technol, Taipa 999078, Madhya Pradesh, Peoples R China. [Wang, Jun] Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China. [Zhang, Guojie] Univ Copenhagen, Ctr Social Evolut, Dept Biol, DK-2100 Copenhagen, Denmark. RP Korb, J (reprint author), Univ Freiburg, D-79117 Freiburg, Germany. EM judith.korb@biologie.uni-freiburg.de; zhanggj@genomics.org.cn; jliebig@asu.edu RI Hunt, Brendan/B-9816-2009; Zhang, Guojie/B-6188-2014; Terrapon, Nicolas/O-3630-2014; Wang, Jun/C-8434-2016; Bornberg-Bauer, Erich/A-1563-2013; Elsik, Christine/C-4120-2017; Wang, Jun/B-9503-2016 OI Hu, Haofu/0000-0001-8145-3009; Childers, Chris/0000-0002-1253-5550; Hunt, Brendan/0000-0002-0030-9302; Zhang, Guojie/0000-0001-6860-1521; Terrapon, Nicolas/0000-0002-3693-6017; Wang, Jun/0000-0002-8540-8931; Bornberg-Bauer, Erich/0000-0002-1826-3576; Elsik, Christine/0000-0002-4248-7713; Huylmans, Ann Kathrin/0000-0001-8871-4961; Li, Cai/0000-0001-7843-2151; Wang, Jun/0000-0002-2113-5874 FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2007-35302-18172]; Deutschen Forschungsgemeinschaft (DFG) [KO1895/6]; LOEWE Research Focus 'Insect Biotechnology' FX We thank the administrators of the Pebble Beach Company for permission to collect termites and Navdeep Mutti for initial help in RNA and DNA sampling. This work was supported by the Agriculture and Food Research Initiative Competitive Grant number 2007-35302-18172 from the USDA National Institute of Food and Agriculture to J.L. and C.S.B.; and a research grant from the Deutschen Forschungsgemeinschaft (DFG) to J.K. (KO1895/6) and LOEWE Research Focus 'Insect Biotechnology' to A.V. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 70 TC 69 Z9 76 U1 8 U2 80 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2014 VL 5 AR 3636 DI 10.1038/ncomms4636 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AJ0RB UT WOS:000337362200001 PM 24845553 ER PT J AU Rasooly, R Hernlem, BJ AF Rasooly, Reuven Hernlem, Bradley J. TI Quantitative Analysis of Staphylococcus Enterotoxin A by Differential Expression of IFN-gamma in Splenocyte and CD4(+) T-Cells SO SENSORS LA English DT Article DE staphylococcal enterotoxins; interferon-gamma; active toxin detection; CD4(+) T-cell; splenocytes ID IN-VITRO; FOOD; ASSAY; OUTBREAK; AUREUS AB Staphylococcus aureus is an important bacterial pathogen that produces a range of Staphylococcal Enterotoxins (SEs) which cause gastroenteritis and superantigen activation of T cells, the mechanism of which is not well understood. The ability to rapidly detect and quantify SEs is very important in order to learn the causes of staphylococcal outbreaks and to stop similar outbreaks in the future. Enzyme-linked immunosorbent assays (ELISAs) have been developed for detection of several SEs. However, these immunological methods cannot distinguish between active and inactive toxin. It is known that interferon-gamma (IFN-gamma) expressed in response to stimulation by SEs contributes significantly to the pathogenesis of S. aureus infection. Nonetheless, the cellular source of IFN-gamma is still unclear and the contributions of the specific splenocyte types. In our effort to understand the immunologic response to Staphylococcal Enterotoxin A (SEA) exposure, we studied IFN-gamma production in mouse splenocytes. We demonstrated that short term ex vivo exposure of splenocytes or primary naive CD4(+) T-cells to biologically active SEA induces differential expression of IFN-gamma mRNA in a time and dose dependent manner and the expression levels reflect the levels of IFN-gamma secreted protein. Positive isolated CD4(+) T-cells accounted for only 10% of IFN-gamma production. We also demonstrate that expression of IFN-gamma can be used for rapid quantitative analysis of active SEA with a detection limit of 1 ng/mL. C1 [Rasooly, Reuven; Hernlem, Bradley J.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Rasooly, R (reprint author), ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov; bradley.hernlem@ars.usda.gov NR 15 TC 2 Z9 2 U1 1 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD MAY PY 2014 VL 14 IS 5 BP 8869 EP 8876 DI 10.3390/s140508869 PG 8 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA AI7WM UT WOS:000337112200066 PM 24854207 ER PT J AU Fernandez-Sainz, IJ Largo, E Gladue, DP Fletcher, P O'Donnell, V Holinka, LG Carey, LB Lu, X Nieva, JL Borca, MV AF Fernandez-Sainz, I. J. Largo, E. Gladue, D. P. Fletcher, P. O'Donnell, V. Holinka, L. G. Carey, L. B. Lu, X. Nieva, J. L. Borca, M. V. TI Effect of specific amino acid substitutions in the putative fusion peptide of structural glycoprotein E2 on Classical Swine Fever Virus replication SO VIROLOGY LA English DT Article DE Fusion peptide; Classical Swine Fever Virus; CSFV; Classical swine fever; Pestivirus ID VIRAL-DIARRHEA-VIRUS; HOG-CHOLERA VIRUS; PROTEINS E-RNS; ENVELOPE GLYCOPROTEIN; TRANSMEMBRANE PROTEINS; VIRULENCE DETERMINANT; STRAIN BRESCIA; CELL-CULTURE; PESTIVIRUS; MODEL AB E2, along with Ems and El, is an envelope glycoprotein of Classical Swine Fever Virus (CSFV). E2 is involved in several virus functions: cell attachment, host range susceptibility and virulence in natural hosts. Here we evaluate the role of a specific E2 region, (818)CPIGWTGVIEC(828), containing a putative fusion peptide (FP) sequence. Reverse genetics utilizing a full-length infectious clone of the highly virulent CSFV strain Brescia (BICv) was used to evaluate how individual amino acid substitutions within this region of E2 may affect replication of BICv. A synthetic peptide representing the complete E2 FP amino acid sequence adopted a beta-type extended conformation in membrane mimetics, penetrated into model membranes, and perturbed lipid bilayer integrity in vitro. Similar peptides harboring amino acid substitutions adopted comparable conformations but exhibited different membrane activities. Therefore, a preliminary characterization of the putative FP (818)CPIGWTGVIEC(828) indicates a membrane fusion activity and a critical role in virus replication. Published by Elsevier Inc. C1 [Fernandez-Sainz, I. J.; Gladue, D. P.; Fletcher, P.; O'Donnell, V.; Holinka, L. G.; Borca, M. V.] ARS, Plum Isl Anim Dis Ctr, USDA, New York, NY 11957 USA. [Largo, E.; Nieva, J. L.] Univ Basque Country UPV EHU, Dept Biochem & Mol Biol, Biophys Unit CSIC UPV EHU, Bilbao 48080, Spain. [O'Donnell, V.; Lu, X.] DHS, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Carey, L. B.] Univ Pompeu Fabra, Dept Expt & Hlth Sci, E-08003 Barcelona, Spain. RP Borca, MV (reprint author), USDA ARS NAA, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM manuel.borca@ars.usda.gov RI Carey, Lucas/L-8547-2014; Fernandez Sainz, Ignacio/I-3046-2016; OI Carey, Lucas/0000-0002-7245-6379; Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178 FU Basque Government [BIO2011-29792, IT838-13]; Spanish MINECO FX This study was in part supported by Spanish MINECO and Basque Government grants (BIO2011-29792 and IT838-13 to J.L.N.). We thank the Plum Island Animal Disease Center animal care unit staff for excellent technical assistance. We specially thank Melanie Prarat for editing the manuscript. NR 38 TC 5 Z9 5 U1 0 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2014 VL 456 BP 121 EP 130 DI 10.1016/j.virol.2014.03.005 PG 10 WC Virology SC Virology GA AI9NF UT WOS:000337258600012 PM 24889231 ER PT J AU Laughlin, MR Bantle, JP Havel, PJ Parks, E Klurfeld, DM Teff, K Maruvada, P AF Laughlin, Maren R. Bantle, John P. Havel, Peter J. Parks, Elizabeth Klurfeld, David M. Teff, Karen Maruvada, Padma TI Clinical Research Strategies for Fructose Metabolism SO ADVANCES IN NUTRITION LA English DT Review ID GLUCOSE-SWEETENED BEVERAGES; DE-NOVO LIPOGENESIS; MONOCYTE CHEMOATTRACTANT PROTEIN-1; PLASMINOGEN-ACTIVATOR INHIBITOR-1; LOW-DENSITY LIPOPROTEINS; FATTY-ACID SYNTHESIS; DIETARY FRUCTOSE; CORN SYRUP; URIC-ACID; HIGH-CARBOHYDRATE AB Fructose and simple sugars are a substantial part of the western diet, and their influence on human health remains controversial. Clinical studies in fructose nutrition have proven very difficult to conduct and interpret. NIH and USDA sponsored a workshop on 13-14 November 2012, "Research Strategies for Fructose Metabolism," to identify important scientific questions and parameters to be considered while designing clinical studies. Research is needed to ascertain whether there is an obesogenic role for fructose-containing sugars via effects on eating behavior and energy balance and whether there is a dose threshold beyond which these sugars promote progression toward diabetes and liver and cardiovascular disease, especially in susceptible populations. Studies tend to fall into 2 categories, and design criteria for each are described. Mechanistic studies are meant to validate observations made in animals or to elucidate the pathways of fructose metabolism in humans. These highly controlled studies often compare the pure monosaccharides glucose and fructose. Other studies are focused on clinically significant disease outcomes or health behaviors attributable to amounts of fructose-containing sugars typically found in the American diet. These are designed to test hypotheses generated from short-term mechanistic or epidemiologic studies and provide data for health policy. Discussion brought out the opinion that, although many mechanistic questions concerning the metabolism of monosaccharide sugars in humans remain to be addressed experimentally in small highly controlled studies, health outcomes research meant to inform health policy should use large, long-term studies using combinations of sugars found in the typical American diet rather than pure fructose or glucose. C1 [Laughlin, Maren R.; Teff, Karen; Maruvada, Padma] NIDDK, NIH, Bethesda, MD 20892 USA. [Bantle, John P.] Univ Minnesota, Dept Med, Div Endocrinol & Diabet, Minneapolis, MN 55455 USA. [Havel, Peter J.] Univ Calif Davis, Sch Vet Med, Dept Nutr, Dept Mol Biosci, Davis, CA 95616 USA. [Parks, Elizabeth] Univ Missouri, Inst Clin Translat Sci, Dept Nutr & Exercise Physiol, Columbia, MO USA. [Klurfeld, David M.] USDA ARS, Beltsville, MD USA. RP Laughlin, MR (reprint author), NIDDK, NIH, Bethesda, MD 20892 USA. OI Parks, Elizabeth/0000-0001-5681-1097 FU National Institutes of Health; USDA; National Institute of Diabetes and Digestive and Kidney Diseases; National Heart Lung and Blood Institute; Merck FX On behalf of the organizers and participants in a 2012 National Institutes of Health and USDA-sponsored workshop, 'clinical Research Strategies for Fructose Metabolism." Funding for the workshop was from the National Institute of Diabetes and Digestive and Kidney Diseases, the National Heart Lung and Blood Institute, and the USDA.; Author disclosures. E. Parks serves on the Atkins Nutritionals Scientific Advisory Board, Merck Speakers Bureau and receives funding from Merck. M. R. Laughlin, J. P Bantle, P. J. Havel, D. M. Klurfeld, K Taft, P. Maruvada, no conflicts of interest NR 76 TC 9 Z9 9 U1 3 U2 29 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2014 VL 5 IS 3 BP 248 EP 259 DI 10.3945/an.113.005249 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AI5CV UT WOS:000336883900004 PM 24829471 ER PT J AU Ribeiro, SML Kehayias, JJ AF Ribeiro, Sandra M. L. Kehayias, Joseph J. TI Sarcopenia and the Analysis of Body Composition SO ADVANCES IN NUTRITION LA English DT Review ID BIOELECTRICAL-IMPEDANCE VECTOR; SKELETAL-MUSCLE; OLDER-ADULTS; PHYSICAL-DISABILITY; ELDERLY SUBJECTS; HEALTH; MASS; FAT; FRAILTY; OBESITY AB Reduction of lean mass is a primary body composition change associated with aging. Because many factors contribute to lean mass reduction, the problem has been given various names depending on the proposed cause, such as "age-related sarcopenia," "dynapenia," "myopenia," "sarcopenic obesity," or simply "sarcopenia." There is currently no consensus on how to best diagnose the reduction of lean mass and its consequences on health. We propose that simple body composition methods can be used to indirectly evaluate sarcopenia, provided that those techniques are validated against the "quality of lean" criterion that associates muscle mass and metabolic function with the components of fat-free mass. Promising field methods include the use of stable isotopes for the evaluation of water compartments and new approaches to bioelectrical impedance analysis, which is also associated with the monitoring of water homeostasis. C1 [Ribeiro, Sandra M. L.] Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, Brazil. [Ribeiro, Sandra M. L.; Kehayias, Joseph J.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Body Composit Lab, Boston, MA 02111 USA. RP Kehayias, JJ (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Body Composit Lab, Boston, MA 02111 USA. FU Foundation for Research Support of the State of Sao Paulo; USDA [1950-51000-071-03S] FX Supported by the Foundation for Research Support of the State of Sao Paulo and USDA grant 1950-51000-071-03S. NR 82 TC 9 Z9 9 U1 1 U2 17 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2014 VL 5 IS 3 BP 260 EP 267 DI 10.3945/an.113.005256 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AI5CV UT WOS:000336883900005 PM 24829472 ER PT J AU Odle, J Adams, SH Vockley, J AF Odle, Jack Adams, Sean H. Vockley, Jerry TI Carnitine SO ADVANCES IN NUTRITION LA English DT Editorial Material C1 [Odle, Jack] N Carolina State Univ, Raleigh, NC 27695 USA. [Adams, Sean H.] USDA, Western Human Nutr Ctr, Davis, CA USA. [Vockley, Jerry] Univ Pittsburgh, Pittsburgh, PA USA. RP Odle, J (reprint author), N Carolina State Univ, Raleigh, NC 27695 USA. EM jack_odle@ncsu.edu OI Vockley, Jerry/0000-0002-8180-6457; Odle, Jack/0000-0003-4965-2096 FU NIDDK NIH HHS [R01 DK078775] NR 2 TC 0 Z9 0 U1 0 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2014 VL 5 IS 3 BP 289 EP 290 DI 10.3945/an.113.005199 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AI5CV UT WOS:000336883900008 PM 24829475 ER PT J AU Allen, LH AF Allen, Lindsay H. TI Micronutrient Research, Programs, and Policy: From Meta-analyses to Metabolomics SO ADVANCES IN NUTRITION LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; LOW-INCOME COUNTRIES; ANIMAL-SOURCE FOODS; FOLIC-ACID; PREGNANCY OUTCOMES; IMPROVES GROWTH; BIRTH-WEIGHT; SUPPLEMENTATION; CHILDREN; INTERVENTIONS AB Micronutrient deficiencies are widespread among women and children in undernourished populations. Research has identified effective approaches to their prevention, including supplementation, fortification, and dietary and other public health interventions. These interventions have made tremendous improvements in the quality of life, health, and survival of populations around the world, yet the impact varies by nutrient, population, and the outcomes chosen that reflect nutritionally driven change. The WHO guides governments and agencies toward effective strategies to prevent micronutrient deficiencies in women and children, but these are often informed by imperfect studies with limited measures of impact and the inadequate program evaluations and survey databases produced by the nutrition community. The resulting knowledge gaps limit our ability to discern what interventions are effective, under what conditions, among whom, and perhaps most important, why. However, we are moving into an era of opportunity to apply the tools of modern nutrition science, including improved methods of assessing nutritional status, "omics," bioarchival access, systems biology thinking, and interdisciplinary collaborations, that can deepen and broaden our understanding of how micronutrients affect health, how their deficiencies diminish human capacity, and how interventions can improve the well-being of those in need. Relevant training and greater cross-disciplinary efforts will be required to ensure a cell-to-society approach that can systematically address where, to whom, and how to provide micronutrients in the future. C1 Univ Calif Davis, Western Human Nutr Res Ctr, USDA ARS, Davis, CA 95616 USA. RP Allen, LH (reprint author), Univ Calif Davis, Western Human Nutr Res Ctr, USDA ARS, Davis, CA 95616 USA. EM lindsay.allen@ars.usda.gov NR 44 TC 2 Z9 2 U1 2 U2 15 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2014 VL 5 IS 3 BP 344S EP 351S DI 10.3945/an.113.005421 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AI5CV UT WOS:000336883900019 PM 24829487 ER PT J AU Konstantinidou, V Ruiz, LAD Ordovas, JM AF Konstantinidou, Valentini Daimiel Ruiz, Lidia Angeles Ordovas, Jose M. TI Personalized Nutrition and Cardiovascular Disease Prevention: From Framingham to PREDIMED SO ADVANCES IN NUTRITION LA English DT Article ID POLYUNSATURATED FATTY-ACIDS; TCF7L2 GENE POLYMORPHISMS; MEDITERRANEAN DIET; NUTRIENT INTERACTION; BODY-WEIGHT; ASSOCIATION; RISK; POPULATION; SUSCEPTIBILITY; PROMOTER AB Diet is considered the cornerstone for the prevention of age-related diseases, and a low-fat diet has been considered for decades as the most suitable alternative to achieve this goal. However, mounting evidence supports the efficacy of other alternatives, such as the Mediterranean diet. Nevertheless, it is well known that people present a dramatic range of responses to similar environmental challenges, and it has been shown that some of this variability is rooted in the genome. In fact, this knowledge is driving the field of nutrigenetics. The finding of interactions between diet and genetic variants has led to intense research and debate about the effectiveness of personalized nutrition as a more suitable tool for the prevention of chronic diseases than the traditional 1-size-fits-all recommendations. Here, we provide some of our own examples that illustrate the progression of nutrigenetics through the years, from the initial studies within the Framingham Heart Study, to the most recent use of large consortia, such as the Cohorts for Heart and Aging Research in Genomic Epidemiology, and ending up with large dietary intervention studies, such as the PREDIMED (Prevencion con Dieta Mediterranea) study. These recent approaches are providing more robust and clinically relevant gene diet interactions. Therefore, although the current evidence level of applying genomic information to tailoring is at its early stages, the prospect of widespread incorporation of nutrigenetics to the clinical practice is encouraging. C1 [Konstantinidou, Valentini; Daimiel Ruiz, Lidia Angeles; Ordovas, Jose M.] Madrid Inst Adv Studies Food, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ordovas, Jose M.] Natl Ctr Cardiovasc Res, Madrid, Spain. RP Ordovas, JM (reprint author), Madrid Inst Adv Studies Food, Madrid, Spain. EM jose.ordovas@tufts.edu RI Daimiel-Ruiz, Lidia Angeles/M-7779-2014 OI Daimiel-Ruiz, Lidia Angeles/0000-0001-9898-6629 FU NIH/National Heart, Lung, and Blood Institute [P50HL105185]; NIH/National Institute on Aging [P01AG023394]; Merck Serono; USDA [58-1950-0-014] FX The IUNS and the 20th ICN wish to thank the California Walnut Commission and Mead Johnson Nutrition for generously providing educational grants to support the publication and distribution of proceedings from the 20th ICN. The contents of this supplement are solely the responsibility of the authors and do not necessarily represent official views of the IUNS. The supplement coordinators were Angel Gil, Ibrahim Elmadfa, and Alfredo Martinez The supplement coordinators had no conflicts of interest to disclose.; Supported by NIH/National Heart, Lung, and Blood Institute grant P50HL105185, NIH/National Institute on Aging grant P01AG023394, a research grant from Merck Serono, and the USDA under agreement 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the USDA The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript NR 31 TC 9 Z9 9 U1 1 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2014 VL 5 IS 3 BP 368S EP 371S DI 10.3945/an.113.005686 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AI5CV UT WOS:000336883900022 PM 24829490 ER PT J AU Cook, DE Shinners, KJ Weimer, PJ Muck, RE AF Cook, David E. Shinners, Kevin J. Weimer, Paul J. Muck, Richard E. TI High dry matter whole-plant corn as a biomass feedstock SO BIOMASS & BIOENERGY LA English DT Article DE Harvest; Storage; Corn; Pretreatment; Losses; Stover AB This research investigated the harvest, ambient pre-treatment, and storage of whole-plant corn as an alternative to conventional systems where corn grain and stover are fractionated at harvest. Harvesting the whole-plant, both grain and most of the above ground stover, after physiological maturity can reduce the intense logistics challenges typically associated with corn harvest and expand the harvest window. To determine the feasibility of the proposed system, corn was harvested at 350-840 g kg(-1) whole-plant dry matter (DM) using a forage harvester and then ensiled in pilot-scale silos. Ambient pretreatment during storage was investigated using both dilute acid and lime. Both pretreated and control whole-plant silages were very well conserved during anaerobic storage with DM losses generally less than 40 g kg(-1). Hydrodynamic separation of the grain and stover fractions after storage was found to be more effective at fractionating starch and fiber than conventional dry grain harvest, and both fractions had desirable composition. The effects of pretreatment on the silage were very pronounced at 30 and 100 g (kg DM)(-1) sulfuric acid loading with less than 100 g (kg DM)(-1) of the hemicellulose still bound in the cell wall at DM contents greater than 500 g kg(-1). The whole-plant harvest and storage system was shown to be a viable alternative to conventional corn grain and stover systems for producing feedstocks for biochemical conversion. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Cook, David E.; Shinners, Kevin J.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Weimer, Paul J.; Muck, Richard E.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Shinners, KJ (reprint author), Univ Wisconsin, Dept Biol Syst Engn, 460 Henry Mall, Madison, WI 53706 USA. EM kjshinne@wisc.edu FU University Of Wisconsin College Of Agriculture and Life Sciences; USDAARS US Dairy Forage Research through Specific Cooperative Research Agreement [58-3655-2-128]; Archer-Daniels-Midland FX This research was partially sponsored by the University Of Wisconsin College Of Agriculture and Life Sciences; USDAARS US Dairy Forage Research through Specific Cooperative Research Agreement 58-3655-2-128; and by Archer-Daniels-Midland. This research could not have been completed without the assistance of the staff of the Arlington Agricultural Research Station. NR 13 TC 1 Z9 2 U1 2 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD MAY PY 2014 VL 64 BP 230 EP 236 DI 10.1016/j.biombioe.2014.02.026 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AI3RA UT WOS:000336778400022 ER PT J AU Schmer, MR Dose, HL AF Schmer, Marty R. Dose, Heather L. TI Cob biomass supply for combined heat and power and biofuel in the north central USA SO BIOMASS & BIOENERGY LA English DT Article DE Corn cobs; Biofuel; Biorefinery; Combined heat and power; Ethanol; Life cycle assessment ID WESTERN CORN-BELT; STOVER; SOIL; EFFICIENCY; ECONOMICS; REMOVAL; CARBON AB Corn (Zea mays L.) cobs are being evaluated as a potential bioenergy feedstock for combined heat and power generation (CHP) and conversion into a biofuel. The objective of this study was to determine corn cob availability in north central United States (Minnesota, North Dakota, and South Dakota) using existing corn grain ethanol plants as a proxy for possible future co-located cellulosic ethanol plants. Cob production estimates averaged 6.04 Tg and 8.87 Tg using a 40 km radius area and 80 km radius area, respectively, from existing corn grain ethanol plants. The use of CHP from cobs reduces overall GHG emissions by 60%-65% from existing dry mill ethanol plants. An integrated biorefinery further reduces corn grain ethanol GHG emissions with estimated ranges from 13.9 g CO2 equiv MJ(-1) to 17.4 g CO2 equiv MJ-1. Significant radius area overlap (53% overlap for 40 km radius and 86% overlap for 80 km radius) exists for cob availability between current corn grain ethanol plants in this region suggesting possible cob supply constraints for a mature biofuel industry. A multifeedstock approach will likely be required to meet multiple end user renewable energy requirements for the north central United States. Economic and feedstock logistics models need to account for possible supply constraints under a mature biofuel industry. Published by Elsevier Ltd. C1 [Schmer, Marty R.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Dose, Heather L.] N Dakota State Univ, Fargo, ND 58108 USA. RP Schmer, MR (reprint author), Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Rm 137 Keim Hall,East Campus UNL, Lincoln, NE 68583 USA. EM marty.schmer@ars.usda.gov; heather.dose@my.ndsu.edu NR 29 TC 5 Z9 5 U1 0 U2 9 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD MAY PY 2014 VL 64 BP 321 EP 328 DI 10.1016/j.biombioe.2014.03.051 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AI3RA UT WOS:000336778400031 ER PT J AU Mullen, CA Boateng, AA Schweitzer, D Sparks, K Snell, KD AF Mullen, Charles A. Boateng, Akwasi A. Schweitzer, Dirk Sparks, Kevin Snell, Kristi D. TI Mild pyrolysis of P3HB/switchgrass blends for the production of bio-oil enriched with croto nic acid SO JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS LA English DT Article DE P3HB; Crotonic acid; Pyrolysis; Switchgrass ID POLY(BETA-HYDROXYBUTYRIC ACID); THERMAL-DEGRADATION; SWITCHGRASS; ENERGY; YIELD; BIOPLASTICS; BIOMASS; CROPS AB The mild pyrolysis of switchgrass/poly-3-hydroxybutyrate (P3HB) blends that mimic P3HB-producing switchgrass lines was studied in a pilot scale fluidized bed reactor with the goal of simultaneously producing crotonic acid, a switchgrass-based bio-oil, and bio-char. Factors such as pyrolysis temperature, reactor residence time, flow rate and particle size of the P3HB were studied to determine their effects on the recovery of crotonic acid as a component of the pyrolysis oil produced from the mixture. Crotonic acid yields were maximized at 45 wt% of the input P3HB by using small P3HB particles and a pyrolysis temperature of 375 degrees C. The remaining components of the liquid product were similar to those produced via fast pyrolysis of switchgrass alone. Fractional collection within the condensation system of the pyrolysis process development unit (PDU) did not significantly fractionate crotonic acid more than the total liquids collected. Concentrations of 6 to 10 wt% crotonic acid in the liquids were found in all fractions and crotonic acid was effectively collected by both condensation and electrostatic precipitation suggesting that pyrolysis of P3HB produces crotonic acid in both gas and aerosol phases. Published by Elsevier B.V. C1 [Mullen, Charles A.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Schweitzer, Dirk; Sparks, Kevin; Snell, Kristi D.] Metabolix Inc, Cambridge, MA 02139 USA. RP Mullen, CA (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov FU US Department of Energy's (DOE) Office of Energy Efficiency and Renewable Energy (EERE) [DE-EE0004943] FX The authors thank Dr. Christina Dorado, Craig Einfeldt and Michelle Hall of ERRC for technical assistance. This research was supported by a grant from The US Department of Energy's (DOE) Office of Energy Efficiency and Renewable Energy (EERE) to Metabolix (grant DE-EE0004943). NR 23 TC 11 Z9 11 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2370 EI 1873-250X J9 J ANAL APPL PYROL JI J. Anal. Appl. Pyrolysis PD MAY PY 2014 VL 107 BP 40 EP 45 DI 10.1016/j.jaap.2014.01.022 PG 6 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA AI4WU UT WOS:000336868000006 ER PT J AU Miguel, RE Dungan, RS Reeves, JB AF Miguel, Roberto E. Dungan, Robert S. Reeves, James B., III TI Mid-infrared spectroscopic analysis of chemically bound metalcasting sands SO JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS LA English DT Article DE Foundry; Mid-infrared spectrometry; Molding sand; Organic binder; Resin; Thermal degradation ID FOUNDRY SANDS; GREEN SANDS; PYROLYSIS; SOILS AB Foundries around the world discard millions of tons of sand each year even though they can be beneficially used in manufactured soils and geotechnical applications. Despite their usefulness as an aggregate replacement, some environmental authorities are concerned over potential negative impacts associated with residual organic binders in waste foundry sands (WFSs). In this study, chemically bound molding and core sands were obtained from aluminum, bronze and iron foundries that used alkyd urethane, phenolic urethane, Novolac, and natural organic binders. The aim was to use mid-infrared (MIR) spectrometry to assess binder changes within the sands during the casting process. Bands associated with C H stretching were detected in most WFSs. Mid-infrared spectra and total carbon data demonstrated that organic binders closest to the molten metal interface and subjected to the highest casting temperatures exhibited the most thermal degradation. Our results also provided preliminary evidence that MIR spectroscopy could potentially be used as a method to quantify residual binder in WFSs. Published by Elsevier B.V. C1 [Miguel, Roberto E.] Univ Nacl Ctr Prov Buenos Aires, Ctr Invest & Estudios Ambientales, Becario CONICET, RA-7000 Tandil, Bs As, Argentina. [Dungan, Robert S.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. [Reeves, James B., III] USDA ARS, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. RP Dungan, RS (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 North 3600 East, Kimberly, ID 83341 USA. EM robert.dungan@ars.usda.gov FU CONICET; USDA-ARS Northwest Irrigation and Soils Research Laboratory FX The authors would like to thank the many foundries that participated in this study. The first author would also like to acknowledge the generous support from CONICET and the USDA-ARS Northwest Irrigation and Soils Research Laboratory. NR 15 TC 0 Z9 0 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2370 EI 1873-250X J9 J ANAL APPL PYROL JI J. Anal. Appl. Pyrolysis PD MAY PY 2014 VL 107 BP 332 EP 335 DI 10.1016/j.jaap.2014.02.007 PG 4 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA AI4WU UT WOS:000336868000042 ER PT J AU Price, NPJ Furukawa, T Cheng, F Qi, JZ Chen, WQ Crich, D AF Price, Neil P. J. Furukawa, Takayuki Cheng, Fang Qi, Jianzhao Chen, Wenqing Crich, David TI Biosynthesis of 4-aminoheptose 2-epimers, core structural components of the septacidins and spicamycins SO JOURNAL OF ANTIBIOTICS LA English DT Article DE 4-amino-4-deoxyheptose; biosynthetic pathway; septacidin; spicamycin; synthesis ID D-MANNO-HEPTOSE; PROMYELOCYTIC LEUKEMIA-CELLS; ANTITUMOR AGENT SPICAMYCIN; SIALIC-ACID; DIFFERENTIATION INDUCERS; ANALOGS; DECARBOXYLATION; PATHWAY; SULFUR; HL-60 AB Septacidins and spicamycins are acylated 4-aminoheptosyl-beta-N-glycosides produced by Streptomyces fimbriatus and S. alanosinicus, respectively. Their structures are highly conserved, but differ in the stereochemistry of the 4-aminoheptosyl residues. The origin of this stereochemistry is unknown, but is presumably because of the difference in their biosynthetic pathways. We have synthesized the septacidin 4-aminoheptose to verify the difference between septacidin and spicamycin. Isotopic enrichment studies were undertaken using S. fimbriatus, and show that the septacidin heptose is derived from the pentose phosphate pathway. This indicates conserved pathways leading to the biosynthesis of 4-amino-4-deoxy-L-glucoheptose or 4-amino-4-deoxy-L-manno-heptose. C1 [Price, Neil P. J.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Furukawa, Takayuki; Crich, David] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA. [Cheng, Fang; Qi, Jianzhao; Chen, Wenqing] Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430072, Peoples R China. [Cheng, Fang; Qi, Jianzhao; Chen, Wenqing] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430072, Peoples R China. RP Price, NPJ (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM neil.price@ars.usda.gov FU Developmental Therapeutics Program of the National Cancer Institute, National Institutes of Health [KRN5500] FX NPJP thanks Trina Hartman for excellent technical assistance. We thank the Developmental Therapeutics Program of the National Cancer Institute, National Institutes of Health, for providing us with spicamycin (KRN5500). NR 35 TC 1 Z9 1 U1 1 U2 10 PU JAPAN ANTIBIOTICS RESEARCH ASSOC PI TOKYO PA 2 20 8 KAMIOSAKI SHINAGAWA KU, TOKYO, 141, JAPAN SN 0021-8820 J9 J ANTIBIOT JI J. Antibiot. PD MAY PY 2014 VL 67 IS 5 BP 405 EP 414 DI 10.1038/ja.2014.15 PG 10 WC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy GA AI6WG UT WOS:000337017100010 PM 24643053 ER PT J AU Bragg, DC Walls, M AF Bragg, Don C. Walls, Matthew TI Supporting Debate in the Journal of Forestry SO JOURNAL OF FORESTRY LA English DT Editorial Material ID PACIFIC-NORTHWEST; RESTORATION FRAMEWORK; FEDERAL FORESTS; MANAGEMENT C1 [Bragg, Don C.] USDA, Forest Serv, Monticello, AR USA. RP Bragg, DC (reprint author), USDA, Forest Serv, Monticello, AR USA. EM braggd@safnet.org; wallsm@safnet.org NR 22 TC 0 Z9 0 U1 0 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2014 VL 112 IS 3 BP 255 EP 256 DI 10.5849/jof.14-990 PG 2 WC Forestry SC Forestry GA AI2UT UT WOS:000336714300001 ER PT J AU Kenefic, LS AF Kenefic, Laura S. TI Selective Cuffing, Rehabilitation, and Alternatives for Forests of Northeastern North America and Elsewhere SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 USDA, No Res Stn, Forest Serv, Orono, ME USA. RP Kenefic, LS (reprint author), USDA, No Res Stn, Forest Serv, Orono, ME USA. EM lkenefic@fs.fed.us NR 8 TC 1 Z9 1 U1 0 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2014 VL 112 IS 3 BP 259 EP 260 DI 10.5849/jof.13-036 PG 2 WC Forestry SC Forestry GA AI2UT UT WOS:000336714300004 ER PT J AU Kenefic, LS Bataineh, M Wilson, JS Brissette, JC Nyland, RD AF Kenefic, Laura S. Bataineh, Mohammad Wilson, Jeremy S. Brissette, John C. Nyland, Ralph D. TI Silvicultural Rehabilitation of Cutover Mixedwood Stands SO JOURNAL OF FORESTRY LA English DT Article DE Penobscot Experimental Forest; timber stand improvement; crop tree release; precommercial thinning; northern conifers; red spruce ID FOREST VEGETATION SIMULATOR; DIAMETER-LIMIT; NORTHERN CONIFERS; FIR; RETURNS; GROWTH; SYSTEM; YIELDS AB We investigated rehabilitation of mixedwood stands degraded by exploitative cutting on the Penobscot Experimental Forest in Maine. Three precommercial rehabilitation treatments were applied: control (no rehabilitation), moderate rehabilitation (crop tree release [CTR]) and intensive rehabilitation (CTR, timber stand improvement [TSI], and red spruce fill planting). Crop trees (primarily red maple, paper birch, spruce, aspen, and eastern hemlock) were selected and released based on their potential for improved growth and value, spacing, and species composition. Rehabilitation reduced sapling basal area, density, and hardwood abundance and increased crop tree diameter increment. Fill planting increased spruce stocking, but many planted seedlings were browsed. Long-term projections suggested that future stand value will repay costs of moderate rehabilitation. (CTR); intensive rehabilitation (CTR-TSI-planting) as applied in this study requires greater investment than can be repaid through quality and growth improvements of low-value hardwoods and softwoods. Although simulations suggested no difference in future stand value between treated and untreated stands, improvements in composition, growth, and quality after rehabilitation will facilitate later commercial thinning and shelterwood regeneration in stands which otherwise have few management options. C1 [Kenefic, Laura S.] USDA, No Res Stn, Forest Serv, Orono, ME USA. [Bataineh, Mohammad] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Wilson, Jeremy S.] Harris Ctr, Hancock, NH USA. [Nyland, Ralph D.] SUNY Coll Environm Sci & Forestry, Syracuse, NY USA. RP Kenefic, LS (reprint author), USDA, No Res Stn, Forest Serv, Orono, ME USA. EM lkenefic@fs.fed.us; mohammad.albataineh@maine.edu; wilson@harriscenter.org; jbrissette@fs.fed.us; rnyland@syr.edu FU Northeastern States Research Cooperative; USDA Forest Service; Northern Research Station; University of Maine, School of Forest Resources FX Funding for this research was provided by the Northeastern States Research Cooperative, the USDA Forest Service, Northern Research Station, and the University of Maine, School of Forest Resources. In-kind support was provided by Greg Adams of J.D. Irving, Ltd. Jamie Weaver, Sophia Demaio, Julia Guiterman, Jack Kelly, Karl Buckley, Nalbert Tero, Peter McBride, Jacob Hicks, Gordon Sloat, Andrew Wopat, Kate Carey, Mallory Bussell, Brittany Ross, Eric Mauricette, Elizabeth Lewis, Elizabeth Olson, Jeffrey Lombardo, and Justin Waskiewicz of the University of Maine and Richard Dionne of the USDA Forest Service assisted with installation of the experiment, treatment application, sampling, and/or data and metadata management. Rob Lilieholm of the University of Maine assisted with financial analysis. Helpful reviews of an earlier version of this article were provided by Steve Bedard, Patricia Raymond, and Stephane Tremblay of the Quebec Ministry of Natural Resources, Matthew Russell of the University of Minnesota, and anonymous reviewers. NR 65 TC 2 Z9 2 U1 0 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2014 VL 112 IS 3 BP 261 EP 271 DI 10.5849/jof.13-033 PG 11 WC Forestry SC Forestry GA AI2UT UT WOS:000336714300005 ER PT J AU Bragg, DC Darling, OH AF Bragg, Don C. Darling, O. H.Doogie TI Knowledge Transfer to 4-H Youths at the Crossett Experimental Forest SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Bragg, Don C.] US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. RP Bragg, DC (reprint author), US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. EM braggd@safnet.org; ohdpmd@windstream.net NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2014 VL 112 IS 3 BP 316 EP 316 PG 1 WC Forestry SC Forestry GA AI2UT UT WOS:000336714300012 ER PT J AU Sacheck, JM Rasmussen, HM Hall, MM Kafka, T Blumberg, JB Economos, CD AF Sacheck, Jennifer M. Rasmussen, Helen M. Hall, Meghan M. Kafka, Tamar Blumberg, Jeffrey B. Economos, Christina D. TI The Association Between Pregame Snacks and Exercise Intensity, Stress, and Fatigue in Children SO PEDIATRIC EXERCISE SCIENCE LA English DT Article DE cortisol; salivary IgA; accelerometry; blood glucose; glycemic index ID TEAM GAMES PLAYERS; INTERMITTENT ENDURANCE CAPACITY; PREEXERCISE CARBOHYDRATE MEALS; DIFFERENT GLYCEMIC INDEXES; SALIVARY IMMUNOGLOBULIN-A; PROLONGED EXERCISE; PHYSICAL-ACTIVITY; SPRINT PERFORMANCE; IMMUNE-RESPONSE; PLASMA CYTOKINE AB To investigate the association between pregame snacks varying in macronutrient content and exercise intensity, physiological stress, and fatigue in young soccer players. One hour before a 50-min soccer game, children (n = 79; 9.1 +/- 0.8 y) were randomly assigned to consume a raisin-, peanut-butter-, or cereal-based snack. Body mass index, blood glucose, and salivary measures of stress (cortisol and immunoglobulin A-IgA) were measured pre- and post-game. Exercise intensity was measured by accelerometry. Self-administered questionnaires were used to assess diet quality and fatigue. Analysis of covariance was used to examine the relationship between pregame snacks and biochemical outcomes. Postgame glucose and cortisol increased [12.9 +/- 21.3 mg/dL (p <.001) and 0.04 +/- 0.10 mu g/dL (p <.05), respectively] and IgA decreased (-2.3 +/- 9.6 mu g/mL; p <.001) from pregame values. The pregame snack was not associated with exercise intensity or post-game outcome; however, children consuming the cereal-based (high-sugar and high-glycemic index (GI)) snack exercised more intensely than the 2 lower-GI snack groups (p <.05). Children who consumed the high-sugar, high-GI snack also reported more symptoms of fatigue (p <.05). A high-sugar, high-GI pregame snack was associated with exercise intensity and fatigue but not changes in blood sugar or stress biomarkers following a soccer game in children. C1 [Sacheck, Jennifer M.; Hall, Meghan M.; Kafka, Tamar; Economos, Christina D.] Tufts Univ, Friedman Sch, Boston, MA 02111 USA. [Rasmussen, Helen M.; Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Sacheck, JM (reprint author), Tufts Univ, Friedman Sch, Boston, MA 02111 USA. EM jennifer.sacheck@tufts.edu FU California Raisin Marketing Board FX This study was funded by the California Raisin Marketing Board. NR 56 TC 1 Z9 1 U1 1 U2 8 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 0899-8493 EI 1543-2920 J9 PEDIATR EXERC SCI JI Pediatr. Exerc. Sci. PD MAY PY 2014 VL 26 IS 2 BP 159 EP 167 DI 10.1123/pes.2013-0067 PG 9 WC Pediatrics; Physiology; Sport Sciences SC Pediatrics; Physiology; Sport Sciences GA AI6UJ UT WOS:000337012200006 PM 24091353 ER PT J AU Millar, CI Westfall, RD Delany, DL AF Millar, Constance I. Westfall, Robert D. Delany, Diane L. TI Thermal Regimes and Snowpack Relations of Periglacial Talus Slopes, Sierra Nevada, California, USA SO ARCTIC ANTARCTIC AND ALPINE RESEARCH LA English DT Article ID PIKAS OCHOTONA-PRINCEPS; CLIMATIC RELATIONSHIPS; SWISS ALPS; PERMAFROST DISTRIBUTION; ROCK GLACIERS; ACTIVE LAYER; GREAT-BASIN; MOUNTAIN; IMPACTS; CLASSIFICATION AB Thermal regimes of eight periglacial talus slopes, at contrasting elevations, aspects, and substrates, in the Sierra Nevada, California, had complex microclimatic patterns partially decoupled from external conditions. Over three years,. warm seasons showed mean talus matrix temperatures and daily variances lower than surfaces and cooler than free-air; talus surface and matrix positions low in the taluses were colder than higher positions, yielding highly positive altitudinal temperature differentials; ground surface temperatures had greater daily extremes than talus positions; and talus matrix temperatures lagged in response to surface temperature changes. Regulating processes in summer include evaporative cooling, cold-air drainage and Balch effect, and shading effects. In the cold season, talus matrices were warmer than surfaces; low talus positions were warmer than high; isothermal zero-curtain periods occurred before snow disappearance; and snow covered talus low positions more often and longer than higher in the taluses, which were often snow-free. Winter thermal processes likely include insulation from snow cover at talus bases, free exchange between talus matrix and external air in the upper talus, and latent heat from thaw-refreezing in late winter. Permanent ice may occur within high elevation talus slopes. Partially de,coupled talus thermal regimes provide buffered habitats for mammals such as American pikas and are likely to be important refugia under future warming. C1 [Millar, Constance I.; Westfall, Robert D.; Delany, Diane L.] USDA Forest Serv, Pacific Southwest Res Stn, Albany, CA 94710 USA. RP Millar, CI (reprint author), USDA Forest Serv, Pacific Southwest Res Stn, 800 Buchanan St WAB, Albany, CA 94710 USA. EM cmillar@fs.fed.us NR 66 TC 7 Z9 7 U1 4 U2 28 PU INST ARCTIC ALPINE RES PI BOULDER PA UNIV COLORADO, BOULDER, CO 80309 USA SN 1523-0430 EI 1938-4246 J9 ARCT ANTARCT ALP RES JI Arct. Antarct. Alp. Res. PD MAY PY 2014 VL 46 IS 2 BP 483 EP 504 DI 10.1657/1938-4246-46.2.483 PG 22 WC Environmental Sciences; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA AI3RB UT WOS:000336778500017 ER PT J AU Holsinger, L Keane, RE Isaak, DJ Eby, L Young, MK AF Holsinger, Lisa Keane, Robert E. Isaak, Daniel J. Eby, Lisa Young, Michael K. TI Relative effects of climate change and wildfires on stream temperatures: a simulation modeling approach in a Rocky Mountain watershed SO CLIMATIC CHANGE LA English DT Article ID WESTERN UNITED-STATES; RIVER-BASIN; FIRE; ECOSYSTEMS; SEVERITY; MONTANA; HABITAT; LANDSCAPES; MANAGEMENT; RESPONSES AB Freshwater ecosystems are warming globally from the direct effects of climate change on air temperature and hydrology and the indirect effects on near-stream vegetation. In fire-prone landscapes, vegetative change may be especially rapid and cause significant local stream temperature increases but the importance of these increases relative to broader changes associated with air temperature and hydrology are not well understood. We linked a spatially explicit landscape fire and vegetation model (FireBGCv2) to an empirical regression equation that predicted daily stream temperatures to explore how climate change and its impacts on fire might affect stream thermal conditions across a partially forested, mountainous landscape in the western U.S. We used the model to understand the roles that wildfire and management actions such as fuel reduction and fire suppression could play in mitigating stream thermal responses to climate change. Results indicate that air temperature increases associated with future climates could account for a much larger proportion of stream temperature increases (as much as 90 % at a basin scale) than wildfire. Similarly, land management scenarios that limited wildfire prevalence had negligible effects on future stream temperature increases. These patterns emerged at broader spatial scales because wildfires typically affected only a subset of a stream's network. However, at finer spatial and temporal scales stream temperatures were sensitive to wildfire. Although wildfires will continue to cause local, short-term effects on stream temperatures, managers of aquatic systems may need to find other solutions to cope with the larger impact from climate change on future stream warming that involves adapting to the increases while developing broad strategies for riparian vegetation restoration. C1 [Holsinger, Lisa; Keane, Robert E.] US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT USA. [Isaak, Daniel J.] US Forest Serv, Rocky Mt Res Stn, Boise Aquat Sci Lab, Boise, ID USA. [Eby, Lisa] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Young, Michael K.] US Forest Serv, Rocky Mt Res Stn, Forestry Sci Lab, Missoula, MT USA. RP Holsinger, L (reprint author), US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT USA. EM lisamholsinger@fs.fed.us FU Joint Fire Sciences Program [JFSP-09-1002-9] FX This research was funded by the Joint Fire Sciences Program under JFSP-09-1002-9. We thank Violet Holley, Christie Lowney, Aaron Sparks, Signe Leirfallom, Robin Silverstein, and Pamela Siknink (USFS) for field assistance, Olga Helmy (University of Montana), Chris Clancy and Leslie Nyce (Montana Fish, Wildlife and Parks), and Mike Jakober (USFS) for stream temperature data. We thank Mary Manning (USFS) for her contributions to study design and riparian vegetation sampling, and Ruth Wooding and Melissa Wegner (USFS) for their local knowledge and assistance. NR 60 TC 7 Z9 8 U1 4 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD MAY PY 2014 VL 124 IS 1-2 BP 191 EP 206 DI 10.1007/s10584-014-1092-5 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AH8DW UT WOS:000336365300015 ER PT J AU Mahan, AL Murray, SC Crosby, K Scott, MP AF Mahan, Adam L. Murray, Seth C. Crosby, Kevin Scott, M. Paul TI Quality Protein Maize Germplasm Characterized for Amino Acid Profiles and Endosperm Opacity SO CROP SCIENCE LA English DT Article ID ZEA-MAYS-L; NUTRITIONAL-VALUE; GRAIN METHIONINE; REGISTRATION; SELECTION; VARIABILITY; PERFORMANCE; TRYPTOPHAN; GENOTYPES; INBREDS AB Quality protein maize (QPM) is improved over normal (non-QPM) maize in grain concentrations of the essential amino acids lysine and tryptophan. Quality protein maize has a long history as tropical adapted germplasm, but little effort has been made to incorporate temperate or subtropical germplasm for temperate adaptation and interactions between different modifier loci in these backgrounds are poorly understood. A design-II mating scheme including new temperate and subtropical lines produced 69 hybrids. Large hybrid genetic variation components resulted in substantial broad-sense heritability H-2 estimates, specifically tryptophan (0.46) and endosperm opacity (0.82). A microbial assay for amino acid estimation proved robust across diverse environments with minimal genotype x environment (GxE) effects. Endosperm opacity had no GxE effects across both Texas and Iowa locations demonstrating stability for this trait. Endosperm opacity primarily followed an additive, midparent trend, with a few hybrids deviating from the trend (36%) suggesting a complex nature of multiple modifier loci across diverse germplasm. The top QPM hybrid outperformed the top commercial hybrid by 35 and 30% for lysine and tryptophan as a proportion of grain, respectively. QPM line Tx832 was a parent of top hybrids for lysine and tryptophan, and the highest noncommercial hybrids for methionine. Minimal correlations with yield and other traits suggest that future breeding should result in QPM hybrids with increasingly competitive yields. C1 [Mahan, Adam L.; Murray, Seth C.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Crosby, Kevin] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. RP Murray, SC (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM sethmurray@tamu.edu FU Texas Corn Producers; Texas AgriLife Research; Monsanto Fellowship in Plant Breeding FX Support for this project was provided by Texas Corn Producers, Texas AgriLife Research, and a Monsanto Fellowship in Plant Breeding to Adam Mahan. Dr. Arnell Hallauer kindly provided seed and Beto Garza with AgriLife research station in Weslaco assisted with field trials. Assistance with all aspects of work was appreciated from staff, graduate students, and undergraduate student workers. NR 50 TC 2 Z9 2 U1 2 U2 19 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 863 EP 872 DI 10.2135/cropsci2012.11.0658 PG 10 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200001 ER PT J AU Santa-Cruz, JH Kump, KL Arellano, C Goodman, MM Krakowsky, MD Holland, JB Balint-Kurti, PJ AF Santa-Cruz, Jose H. Kump, Kristen L. Arellano, Consuelo Goodman, Major M. Krakowsky, Matthew D. Holland, James B. Balint-Kurti, Peter J. TI Yield Effects of Two Southern Leaf Blight Resistance Loci in Maize Hybrids SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCI; MAYDIS RACE-O; COCHLIOBOLUS-HETEROSTROPHUS; ARABIDOPSIS-THALIANA; DISEASE RESISTANCE; FITNESS COSTS; BIPOLARIS-MAYDIS; PARENTAL LINES; INHERITANCE; POPULATION AB In this study we examined the effects of two quantitative trait loci (QTL) for southern leaf blight (SLB) resistance on several agronomic traits including disease resistance and yield. B73-3B and B73-6A are two near-isogenic lines (NILs) in the background of the maize (Zea mays L.) inbred B73, each carrying one introgression (called 3B and 6A respectively) encompassing a QTL for SLB resistance. Sets of isohybrid triplets were developed by crossing B73, B73-3B, and B73-6A to several inbred lines. A subset of these triplets for which the B73-3B and/or B73-6A hybrid was significantly more SLB resistant than the B73 check hybrid was selected and assessed in multi-environment yield trials with and without disease. In the presence of SLB, 3B was associated with an approximately 3% yield increase over B73. 6A was associated with a yield advantage in the presence of SLB in specific pedigrees where the 6A resistance phenotype was highly expressed. Results suggested that both introgressions might confer a yield cost in the absence of SLB, but only introgression 6A was associated with a statistically significant reduction. We present evidence to suggest that the yield cost is associated with the resistance phenotype rather than with linkage drag. C1 [Santa-Cruz, Jose H.; Balint-Kurti, Peter J.] NC State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Kump, Kristen L.; Goodman, Major M.; Krakowsky, Matthew D.; Holland, James B.] NC State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Arellano, Consuelo] NC State Univ, Dept Stat, Raleigh, NC 27695 USA. [Krakowsky, Matthew D.; Holland, James B.; Balint-Kurti, Peter J.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Balint-Kurti, PJ (reprint author), NC State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. EM pjbalint@ncsu.edu OI Holland, James/0000-0002-4341-9675; Balint-Kurti, Peter/0000-0002-3916-194X FU USDA-ARS; NCSU; Monsanto, Inc FX The authors would like to thank the following people who helped with various aspects of the research: William Hill, Wayne Dillard, Dale Dowden, Abbey Sutton, David Rhyne, and Shannon Sermons. We thank Cathy Herring and the staff of Central Crops Research Station, the NCSU Sandhills Research Station, the Peanut Belt Research Station, the Cunningham Research Station, and the staff of 27 Farms in Homestead, FL. for their expert help. We acknowledge genotyping help from Pioneer Hi-Bred, in particular Petra Wolters and Mark Jung. This work was funded by the USDA-ARS and NCSU. J. Santa-Cruz's fellowship was funded by Monsanto, Inc. NR 41 TC 0 Z9 0 U1 0 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 882 EP 894 DI 10.2135/cropsci2013.08.0553 PG 13 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200003 ER PT J AU Jernigan, K Smith, CW Hequet, E Beyer, B Percy, R AF Jernigan, Kendra Smith, C. Wayne Hequet, Eric Beyer, Benjamin Percy, Richard TI Combining Ability and Variability for Fiber Maturity among Diverse World Cotton Genotypes SO CROP SCIENCE LA English DT Article ID LINEAR DENSITY FINENESS; UPGRADED COTTONSCAN(TM) INSTRUMENT; LINT SAMPLES; REGISTRATION AB Increased U.S. export of cotton and global competition necessitates that plant breeders continue to improve fiber properties of upland cotton, Gossypium hirsutum (L.). TAM B182-33 ELS (Extra-Long Staple) (Smith, et al., 2009) germplasm line of upland cotton, and 'Tamcot CAMD-E' (Bird, 1979), a short-staple obsolete cultivar, were crossed with 12 cultivars from China, seven from northern Africa, 10 from southern Africa, and seven from the United States. Parents and 72 F(1)s were grown in College Station, TX, in a Line x Tester design during the summers of 2010 and 2011. Mature, unopened bolls were hand harvested, deburred and allowed to dry in limited light. Maturity ratio (M-R) and ribbon width (RbWth) were determined on a Cottonscope at the Fiber and Biopolymer Research Institute (FBRI) in Lubbock, TX on the 38 parents and F(1)s, and general and specific combining abilities were determined. Genetic variation existed for M-R and RbWth among the distinct germplasm pools utilized in this study. 'Allen 333-61 CB 4027' (northern Africa), 'Phytogen 72' (United States), 'UK 64' (southern Africa) and 'Lintsing Sze Tze 4B' (China) and their F 1 progenies from crosses with TAM B182-33 ELS and Tamcot CAMD-E had enhanced maturity characteristics, particularly high M-R values, indicating that their fibers are more mature than that from some of the other cultivars. Data suggest that M-R could be improved through breeding and use of the Cottonscope. C1 [Jernigan, Kendra] Washington State Univ, Dep Crop & Soil Sci, Pullman, WA 99163 USA. [Smith, C. Wayne] Texas A&M Univ, Soil & Crop Sci Dept, College Stn, TX 77843 USA. [Hequet, Eric] Texas Tech Univ, Fiber & Biopolymer Res Inst, Lubbock, TX 79049 USA. [Beyer, Benjamin] Avanta US Inc, Hereford, TX 79045 USA. [Percy, Richard] USDA ARS, Crop Germplasm Res Unit, College Stn, TX 77845 USA. RP Jernigan, K (reprint author), Washington State Univ, Dep Crop & Soil Sci, Pullman, WA 99163 USA. EM Kendra.jernigan@email.wsu.edu RI Hequet, Eric/A-9802-2008 NR 27 TC 0 Z9 0 U1 1 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 906 EP 913 DI 10.2135/cropsci2013.09.0584 PG 8 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200005 ER PT J AU Mukeshimana, G Butare, L Cregan, PB Blair, MW Kelly, JD AF Mukeshimana, Gerardine Butare, Louis Cregan, Perry B. Blair, Matthew W. Kelly, James D. TI Quantitative Trait Loci Associated with Drought Tolerance in Common Bean SO CROP SCIENCE LA English DT Article ID PHASEOLUS-VULGARIS L.; INTEGRATED LINKAGE MAP; AGRONOMIC TRAITS; GROWTH HABIT; GENE POOL; DIFFERENTIAL ADAPTATION; INTERRACIAL POPULATIONS; ABIOTIC STRESS; GAS-EXCHANGE; 2 VARIETIES AB Drought stress is the major limitation of common bean (Phaseolus vulgaris L.) grown in subsistence farming systems worldwide. The objective of this study was to use single nucleotide polymorphism (SNP) markers from the BARCBean6K_3 Beadchip to identify quantitative trait loci (QTL) associated with traits related to drought tolerance in common bean. An intergene pool recombinant inbred line (RIL) population from a cross of drought tolerant line SEA5 and CAL96 cultivar was evaluated in Rwanda for 3 yr under drought stress and nonstress and in Colombia for 1 yr under drought stress. Traits evaluated included the numbers of days to flower, maturity, and seed fill; harvest index and pod harvest index; yield and yield components including number of pods per plant, seeds per pod, 100 seed weight (SW), and seed yield per day. Harvest indices, and SW were stable regardless of water treatment while number of pods per plant, seeds per pod, and yield were significantly reduced by drought. A linkage map of the RIL population spanning 1351 cM was constructed using 2122 SNP markers. The map covered all eleven bean chromosomes with an average distance of 0.64 cM between markers. A total of 14 QTL for performance under drought were consistently identified in different environments. Quantitative trait loci associated with phenology and SW traits mapped near previously reported QTL. Linkage between SW and yield QTL SY3.3(SC) was observed on Pv03 and could be used to simultaneously select for seed yield and size in intergene pool crosses of common bean. C1 [Mukeshimana, Gerardine; Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Butare, Louis] Rwanda Agr Board, Kigali, Rwanda. [Cregan, Perry B.] ARS, USDA, Soybean Genom & Improvement Lab, BARC, Beltsville, MD USA. [Blair, Matthew W.] Tennessee State Univ, Dept Agr & Environm Sci, Nashville, TN 37209 USA. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu FU Borlaug LEAP program; United States Agency for International Development (USAID) [EDH-A-00-07-00005-00]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2009-01929] FX We wish to acknowledge Dr. S. Beebe and the Centro Internacional de Agricultura Tropical (CIAT) for providing the mapping population used in this study. Research was supported by the Borlaug LEAP program and by funds from the United States Agency for International Development (USAID) through the Dry Grain Pulses Collaborative Research Support Program (Cooperative Agreement EDH-A-00-07-00005-00). This project was also supported by Agriculture and Food Research Initiative Competitive Grant no. 2009-01929 from the USDA National Institute of Food and Agriculture. The content is solely the responsibility of the author(s) and does not necessarily represent the official views of the U.S. Agency for International Development (USAID). NR 72 TC 14 Z9 14 U1 1 U2 22 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 923 EP 938 DI 10.2135/cropsci2013.06.0427 PG 16 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200007 ER PT J AU Recker, JR Burton, JW Cardinal, A Miranda, L AF Recker, Jill R. Burton, Joseph W. Cardinal, Andrea Miranda, Lilian TI Genetic and Phenotypic Correlations of Quantitative Traits in Two Long-Term, Randomly Mated Soybean Populations SO CROP SCIENCE LA English DT Article ID RECURRENT SELECTION; HIGH-PROTEIN; REGISTRATION; YIELD; SEED; GERMPLASM; LINES; OIL AB The genetic effects of long-term random mating and natural selection aided by genetic male sterility were evaluated in two soybean [Glycine max (L.) Merr.] populations designated RSII and RSIII. These populations were evaluated in the field at three locations, each with two replications. Genotypic and phenotypic correlations were estimated to determine the effects of 26 generations of random mating. Data was collected on flowering date (R2), maturity date (R8), plant height, lodging, yield, seed weight, protein concentration, and oil concentration. The genotypic correlation between yield and protein was not significant and small (vertical bar 0.5 vertical bar). As these populations were randomly mated for many generations, linkage phase disequilibrium is likely close to zero; therefore, it was concluded that pleiotropic effects, probably caused by interrelated physiological processes, maintain the negative association between oil and protein. The genetic correlations between yield and protein or oil in these populations, however, do suggest that with the right breeding scheme, the negative associations can be reduced and allow for improvement in more than one trait simultaneously. C1 [Recker, Jill R.; Cardinal, Andrea] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Burton, Joseph W.] N Carolina State Univ, USDA ARS, Raleigh, NC 27607 USA. [Miranda, Lilian] Bayer Crop Sci, Morrisville, NC 27560 USA. RP Miranda, L (reprint author), Bayer Crop Sci, 407 Davis Dr, Morrisville, NC 27560 USA. EM lilian.miranda@bayer.com FU United Soybean Board FX We thank Dr. David Dickey, Statistics Department North Carolina State University, for his help and guidance in analyzing the data for these experiments. Also, we thank the technicians and employees of the Soybean and Nitrogen Fixation Research Unit for their help in collecting data and managing trials. This research was supported in part by the United Soybean Board. NR 20 TC 3 Z9 3 U1 1 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 939 EP 943 DI 10.2135/cropsci2013.07.0447 PG 5 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200008 ER PT J AU Carter, AH Cambron, SE Ohm, HW Bosque-Perez, NA Kidwell, KK AF Carter, A. H. Cambron, S. E. Ohm, H. W. Bosque-Perez, N. A. Kidwell, K. K. TI Hessian Fly (Mayetiola destructor [Say]) Resistance Identified on Chromosome 1AS in the Spring Wheat (Triticum aestivum L.) Cultivar 'Louise' SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCI; UNITED-STATES; GENE; CECIDOMYIIDAE; DIPTERA; POPULATIONS; VIRULENCE; LINKAGE; MARKERS; INTERRELATIONSHIPS AB The Hessian fly (Mayetiola destructor [Say]) is a major yield-limiting pest in wheat (Triticum aestivum L.) production systems. Resistant varieties provide the most effective control method. The objectives of this study were to identify the chromosomal location of and molecular markers associated with the Hessian fly resistance from the spring wheat cultivar 'Louise'. One hundred eighty-eight F-5:6 recombinant inbred lines (RILs) from a Louise (resistant) x 'Penawawa' (susceptible) cross were evaluated for resistance to Hessian fly biotype C and a mixed population originating from field collections in the Pacific Northwest (PNW). Eight to 15 plants per RIL were scored for resistance at Purdue University and the University of Idaho. Data were converted to percentages of susceptible plants within each RIL. Louise and Penawawa had averaged susceptibility values of 15 and 100%, respectively. The RIL population ranged from 0 to 100% susceptible. A genetic linkage map consisting of 320 simple sequence repeat and 8530 single nucleotide polymorphism DNA markers was used for quantitative trait locus (QTL) analysis. One QTL, identified as QHf.wak-1A, was significantly associated with Hessian fly resistance at an average LOD of 26.2 and accounted for 77% of the phenotypic variation. QHf.wak-1A was localized to a 4.6-cM region on chromosome 1AS, flanked by markers Xiwb70039 and Xcfd15. Marker, biotype screening, and allelism results indicate that this QTL is different from H9, H11, H5, and H3, but linked to H3 and H5. These data continue to clarify the relationship between Hessian fly genes on chromosome 1AS and would facilitate breeding efforts. C1 [Carter, A. H.; Kidwell, K. K.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Cambron, S. E.] ARS, USDA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Ohm, H. W.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Bosque-Perez, N. A.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. RP Carter, AH (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM ahcarter@wsu.edu FU National Research Initiative of USDA's Cooperative State Research, Education and Extension Service, CAP [2006-55606-16629]; USDA National Institute of Food and Agriculture, and Washington State University [2011-68002-30029] FX This research was supported by the National Research Initiative of USDA's Cooperative State Research, Education and Extension Service, CAP Grant No. 2006-55606-16629, the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, and Washington State University. We thank Lana Unger at the University of Idaho for technical assistance. We thank Vic DeMacon for assistance with developing the mapping population, and Gary Shelton for supplying seeds of resistant germplasm and for deciphering the pedigree of Louise. NR 48 TC 0 Z9 0 U1 1 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 971 EP 981 DI 10.2135/cropsci2012.11.0636 PG 11 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200012 ER PT J AU Case, AJ Skinner, DZ Garland-Campbell, KA Carter, AH AF Case, Austin J. Skinner, Daniel Z. Garland-Campbell, Kimberly A. Carter, Arron H. TI Freezing Tolerance-Associated Quantitative Trait Loci in the Brundage x Coda Wheat Recombinant Inbred Line Population SO CROP SCIENCE LA English DT Article ID TRITICUM-AESTIVUM-L.; LOW-TEMPERATURE TOLERANCE; GENE-EXPRESSION ANALYSIS; WINTER-WHEAT; FROST-TOLERANCE; CHROMOSOME 5A; SATURATED SOIL; COLD-ACCLIMATION; FLOWERING TIME; FR1 GENES AB Freezing tolerance is an essential trait for winter wheat cultivars. A genetic analysis of a Brundage x Coda winter wheat recombinant inbred line (RIL) mapping population was undertaken to identify quantitative trait loci (QTL) associated with freezing tolerance. Five-week to 6-wk old, cold-acclimated plants were frozen to -10.5, -11.5, or -12.5 degrees C. The standardized mean percentage survival of all RILs within each temperature was 61, 44, and 28%, respectively. A total of 2391 polymorphic DNA markers including 1984 single nucleotide polymorphism (SNP), 232 Diversity Array Technology (DArT), and 175 simple sequence repeat (SSR) markers were used to create a genome-wide genetic linkage map. The QTL analysis identified six QTL that were associated with freezing tolerance at either a specific temperature or a combination of temperatures. The QTL QFrbr.wak-5A was associated with freezing tolerance at all temperatures tested and was on chromosome 5AL. Further marker analysis indicates that this QTL is not an effect of known sequence polymorphisms at Vrn-A1. On the basis of map homology, QFrbr.wak-5A mapped at or near the CBF (cold binding factor) gene cluster at Fr-A2, but not an effect of TaCBF-A15, TaCBF-A14, or TaCBF-A12. Other QTL were located on chromosomes 2A, 3A, 5B, and 6D, and were significant at only specific temperatures. Identification of QTL associated with freezing tolerance may lead to useful genetic markers for marker-assisted selection, allowing for more efficient development of freezing tolerant cultivars. Additional studies of this QTL will further enhance knowledge of cold tolerance in wheat, as this QTL is not due to known sequence variation at Vrn-A1 or tested CBF genes. C1 [Case, Austin J.; Carter, Arron H.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Skinner, Daniel Z.; Garland-Campbell, Kimberly A.] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Carter, AH (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM ahcarter@wsu.edu FU National Research Initiative Competitive Grants CAP Project from the USDA National Institute of Food and Agriculture, Washington State University [2011-68002-30029]; Washington Grain Commission FX We would like to thank Dr. Jie Zhu for providing us access to her unpublished marker sequences and Brian Bellinger for assistance using the freezing chamber. Our appreciation is extended to Jenny Hansen and the University of Idaho for developing the RIL population. Furthermore, we would like to thank Kerry Balow for green house plant care. The wheat 9K iSelect SNP assay was developed by E. Akhunov and M. Hayden in collaboration with S. Chao, G. Brown-Guedira, M. Sorrells, D. See, and C. Cavanagh. This project was supported by the National Research Initiative Competitive Grants CAP Project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, Washington State University, and the Washington Grain Commission. NR 70 TC 2 Z9 2 U1 2 U2 21 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 982 EP 992 DI 10.2135/cropsci2013.08.0526 PG 11 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200013 ER PT J AU Jernigan, K Smith, CW Hequet, E Beyer, B Percy, R AF Jernigan, Kendra Smith, C. Wayne Hequet, Eric Beyer, Benjamin Percy, Richard TI Combining Ability and Variability for Fiber Color among Diverse Cotton Genotypes SO CROP SCIENCE LA English DT Article ID REGISTRATION AB Cotton (Gossypium hirsutum L.) fiber color is influenced by biotic and abiotic factors as well as genetics. After boll opening, exposure to the elements modifies fiber color; fibers may become grayer (excessive moisture leading to biotic activity) and lose luster (lower reflectance). Excessive weathering will lead to poorer processing efficiency and lower dye uptake. Genetically whiter cotton fibers could result in reduced use of bleaching agents before dyeing, thus lowering production costs and providing a more environmentally-friendly product. Cotton cultivars TAM B182-33 ELS (Extra Long Staple) and Tamcot CAMD-E were crossed with 12 cultivars from China, 7 from northern Africa, 10 from southern Africa, and 7 from the United States and grown in a line x tester design at College Station, TX during 2010 and 2011. Seedcotton was harvested by hand on the day of maturity, deburred, and allowed to dry in limited light. Lint was separated into 2-g sub-samples, and color measurements were taken using a Konica-Minolta CR-310 reflectance colorimeter. Absolute color measurements were obtained in the CIE L*a*b* color system. General and specific combining abilities for all the variables were determined. Genetic variation existed for all color parameters measured. The cultivars A 7215 (southern Africa), Tejas (United States), PAN 575 (northern Africa), Lintsing Sze Tze 4B (China), F 280 (northern Africa), and Nanging #12 (China) and their F-1 progenies demonstrated superior whiteness. PAN 575 was the best general combiner for whiteness with the two testers in the study. C1 [Jernigan, Kendra] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99163 USA. [Smith, C. Wayne] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Hequet, Eric] Texas Tech Univ, Fiber & Biopolymer Res Inst, Lubbock, TX 79049 USA. [Beyer, Benjamin] Avanta US Inc, Hereford, TX 79045 USA. [Percy, Richard] USDA ARS, Crop Germplasm Res Unit, College Stn, TX 77845 USA. RP Jernigan, K (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99163 USA. EM kendra.jernigan@email.wsu.edu RI Hequet, Eric/A-9802-2008 NR 20 TC 0 Z9 0 U1 1 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 1041 EP 1047 DI 10.2135/cropsci2013.09.0583 PG 7 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200019 ER PT J AU Orellana, M Edwards, J Carriquiry, A AF Orellana, Massiel Edwards, Jode Carriquiry, Alicia TI Heterogeneous Variances in Multi-Environment Yield Trials for Corn Hybrids SO CROP SCIENCE LA English DT Article ID MIXED LINEAR-MODELS; MAXIMUM-LIKELIHOOD; BAYESIAN-APPROACH; 2-STAGE ANALYSIS; GENOTYPE; PERFORMANCE; DIAGNOSTICS; PREDICTION; REGRESSION; DISPERSION AB Recent developments in statistics and computing have enabled much greater levels of complexity in statistical models of multi-environment yield trial data. One particular feature of interest to breeders is simultaneously modeling heterogeneity of variances among environments and hybrids. Our objective was to estimate the level of heterogeneity of genotype by environment interaction variance and error variance in the Iowa Crop Performance Test for Corn. A Bayesian approach was used to estimate variance components in a hierarchical model that allows for heterogeneous error and genotype-by-environment interaction (GEI) variances applied to corn yield data from the Iowa Crop Performance Test performed between 1995 and 2005. An average of 508 hybrids were tested per year with very little overlap between locations and years, which resulted in a very unbalanced data set. We divided the data into 16 subsets to study the effect of variability across locations and years. We found GEI and error variances to be heterogeneous among both environments and genotypes. Our results for corn contrasted previous work on oat (Avena sativa L.) in which very little heterogeneity was found for error variance among cultivars suggesting that different corn (Zea mays L.) hybrids can have different genotype by environment interaction variances and different error variances. C1 [Orellana, Massiel] Monsanto Co, Creve Coeur, MO 63141 USA. [Edwards, Jode] Iowa State Univ, USDA ARS CICGRU, Dept Agron, Ames, IA 50011 USA. [Carriquiry, Alicia] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. RP Edwards, J (reprint author), Iowa State Univ, USDA ARS CICGRU, Dept Agron, Ames, IA 50011 USA. EM jode.edwards@ars.usda.gov NR 40 TC 1 Z9 1 U1 2 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 1048 EP 1056 DI 10.2135/cropsci2013.09.0653 PG 9 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200020 ER PT J AU Escribano, S Robins, JG AF Escribano, Sandra Robins, Joseph G. TI Phenotypic Performance of Timothy Accessions under Irrigated and Nonirrigated Conditions SO CROP SCIENCE LA English DT Article ID VARYING N NUTRITION; PHLEUM-PRATENSE L.; FORAGE QUALITY; YIELD; GENOTYPES; MANAGEMENT; GERMPLASM; CROP; 1ST AB Timothy (Phleum pratense L.) is one of the most winter-hardy, cool-season perennial grasses used for forage production. However, its lack of tolerance to water stress requires supplemental irrigation for successful production in more arid areas of the world. A set of 112 timothy accessions and 6 check cultivars were evaluated for dry matter yield, in vitro true digestibility, neutral detergent fiber, crude protein, and water soluble carbohydrates across two production years at Lewiston, UT, and Tetonia, ID. Supplemental irrigation was not supplied at Tetonia. For each of the traits except water-soluble carbohydrates, the variance due to the main effect of accessions differed from zero. There was also accession x environment interaction for each of the traits. Within-environment broad-sense heritability estimates differed from zero and ranged from 0.45 +/- 0.16 to 0.80 +/- 0.40. Thus, genetic determinants exerted substantial influence on the expression of the traits in each environment. Most of the commercial cultivars exhibited no benefit over the accessions for these traits, and there was a relationship between the accessions' geographic origin and their potential for timothy improvement. Asian and Northwest and Northeast European accessions possessed the greatest potential for increasing timothy nutritive value under western North American conditions. Yet there was little evidence that any accessions would provide a yield improvement. C1 [Escribano, Sandra] Utah State Univ, Dept Anim Dairy & Vet Sci, Logan, UT 84322 USA. [Robins, Joseph G.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. RP Robins, JG (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. EM Joseph.Robins@ars.usda.gov NR 33 TC 4 Z9 4 U1 1 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 1079 EP 1086 DI 10.2135/cropsci2013.08.0531 PG 8 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200024 ER PT J AU Skinner, RH Stewart, AV AF Skinner, R. Howard Stewart, Alan V. TI Narrow-Leaf Plantain (Plantago lanceolata L.) Selection for Increased Freezing Tolerance SO CROP SCIENCE LA English DT Article ID WINTER-WHEAT; DROUGHT; COLD; CHICORY; CULTIVARS AB Studies in the northeastern United States have shown that the improved cultivars of narrowleaf plantain (Plantago lanceolata L.) do not have sufficient winter hardiness to persist under wintertime conditions typical of that region. However, an experimental line, PG700, developed from productive plants collected from the southern, mid-Atlantic, and midwestern United States had significantly greater survival after freezing than the commercially available cultivars. A field study was initiated in 2006 to select for additional freezing tolerance in PG700. Superior materials collected after two-years of selection pressure were evaluated in growth chambers for survival after exposure to -11 and -14 degrees C or after exposure to drought that was severe enough to kill all visible leaves. The cultivar Tonic was included as a freezing-susceptible control. Survival of spaced plants of PG700 in the field was high, with mortality rates of 9% following the first winter after transplanting, 26% of the remaining plants after the following summer, and 11% after the second winter. Even with the low selection pressure, progeny of plants selected after the first winter had more than twice the survival rate of the original population when exposed to -14 degrees C. No Tonic plants survived at -14 degrees C. PG700 did not have greater drought resistance than Tonic, nor was its drought resistance improved by the selection process. Increased freezing tolerance will increase the suitability of plantain as a component of perennial pasture mixtures in temperate regions of the United States. C1 [Skinner, R. Howard] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Stewart, Alan V.] PGG Wrightson Seeds, Christchurch, New Zealand. RP Skinner, RH (reprint author), ARS, USDA, Pasture Syst & Watershed Management Res Unit, 3702 Curtin Rd, University Pk, PA 16802 USA. EM howard.skinner@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 10 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 1238 EP 1242 DI 10.2135/cropsci2013.06.0390 PG 5 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200043 ER PT J AU Zhang, JL Chen, JL Bowman, BC O'Brien, K Marshall, JM Bonman, JM AF Zhang, Junli Chen, Jianli Bowman, Brian C. O'Brien, Katherine Marshall, Juliet M. Bonman, J. Michael TI Association Mapping of Hagberg Falling Number in Hard White Spring Wheat SO CROP SCIENCE LA English DT Article ID ALPHA-AMYLASE ACTIVITY; SYNTHETIC HEXAPLOID WHEAT; MULTILOCUS GENOTYPE DATA; QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; POPULATION-STRUCTURE; WINTER-WHEAT; RESISTANCE; QUALITY; GRAIN AB Hagberg falling number (FN) is an important quality trait used for grain grading. The FN test can only be measured in the late stages of variety development when progeny are homogeneous and sufficient grain is available for testing. The FN is strongly influenced by environmental conditions during reproductive growth stages, including excess moisture, extreme temperature, and biotic and abiotic stresses. The objective of this study was to identify potential genomic regions that influence FN in a subset of 110 hard white spring (HWS) wheat (Triticum aestivum L.) accessions from the National Small Grain Collection (NSGC) using genome-wide association mapping. The FN tests were conducted using grain flour samples of these accessions grown in five environments. A total of 1740 single nucleotide polymorphism (SNP) markers were used to detect SNP-FN associations using both the general linear model (GLM) and the mixed linear model (MLM). Thirteen quantitative trait loci (QTL) located in nine chromosomal regions were identified in both GLM and MLM approaches. Pyramiding these QTL could explain up to 45% of the phenotypic variation of FN. The present study identified several potential markers for use in marker-assisted selection for high FN lines in wheat breeding. C1 [Zhang, Junli] Univ Calif Davis, Dep Plant Sci, Davis, CA 95616 USA. [Chen, Jianli; Bowman, Brian C.] Univ Idaho, Dep Plant Soil & Entomol Sci, Aberdeen, ID 83210 USA. [O'Brien, Katherine] Univ Idaho, Wheat Qual Lab, Aberdeen, ID 83210 USA. [Marshall, Juliet M.] Univ Idaho, Dept Plant Soil & Entomol Sci, Idaho Falls, ID 83402 USA. [Marshall, Juliet M.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Chen, JL (reprint author), Univ Idaho, Dep Plant Soil & Entomol Sci, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM jchen@uidaho.edu FU USDA National Institute of Food and Agriculture [Triticeae-CAP 2011-68002-30029]; Idaho Wheat Commission; Idaho Agricultural Experimental Station Hatch Projects FX This study was partially supported by the USDA National Institute of Food and Agriculture Triticeae-CAP 2011-68002-30029, the Idaho Wheat Commission, and the Idaho Agricultural Experimental Station Hatch Projects. We would like to thank Dr. Mark Sorrells and Dr. Jorge Dubcovsky for reviewing the manuscript and giving valuable suggestions. We would like also thank the anonymous reviewers for their thorough review and constructive suggestions. NR 53 TC 2 Z9 2 U1 2 U2 17 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2014 VL 54 IS 3 BP 1243 EP 1252 DI 10.2135/cropsci2013.08.0551 PG 10 WC Agronomy SC Agriculture GA AI3FW UT WOS:000336747200044 ER PT J AU Laundre, JW Hernandez, L Medina, PL Campanella, A Lopez-Portillo, J Gonzalez-Romero, A Grajales-Tam, KM Burke, AM Gronemeyer, P Browning, DM AF Laundre, John W. Hernandez, Lucina Lopez Medina, Perla Campanella, Andrea Lopez-Portillo, Jorge Gonzalez-Romero, Alberto Grajales-Tam, Karina M. Burke, Anna M. Gronemeyer, Peg Browning, Dawn M. TI The landscape of fear: the missing link to understand top-down and bottom-up controls of prey abundance? SO ECOLOGY LA English DT Article DE bottom-up control; Chihuahuan Desert; United States and Mexico; fox abundance; Jornada Experimental Range; New Mexico; USA; landscape of fear; Mapimi Biosphere Reserve; Durango; Mexico; Merriami kangaroo rat; Dipodomys merriami; parallel control effects; population density; predation risk; species conservation and management; top-down control ID DESERT RODENT COMMUNITY; PREDATION RISK; PATCH USE; CHIHUAHUAN DESERT; LONG-TERM; ABIOTIC FACTORS; TEMPORAL DYNAMICS; FORAGING GAMES; SNOWSHOE HARES; OWL PREDATION AB Identifying factors that may be responsible for regulating the size of animal populations is a cornerstone in understanding population ecology. The main factors that are thought to influence population size are either resources (bottom-up), or predation (top-down), or interspecific competition (parallel). However, there are highly variable and often contradictory results regarding their relative strengths and influence. These varied results are often interpreted as indicating shifting control among the three main factors, or a complex, nonlinear relationship among environmental variables, resource availability, predation, and competition. We argue here that there is a missing link in our understanding of predator-prey dynamics. We explore whether the landscape-of-fear model can help us clarify the inconsistencies and increase our understanding of the roles, extent, and possible interactions of top-down, bottom-up, and parallel factors on prey population abundance. We propose two main predictions derived from the landscape-of-fear model: (1) for a single species, we suggest that as the makeup of the landscape of fear changes from relatively safe to relatively risky, bottom-up impacts switch from strong to weak as top-down impacts go from weak to strong; (2) for two or more species, interspecific competitive interactions produce various combinations of bottom-up, top-down, and parallel impacts depending on the dominant competing species and whether the landscapes of fear are shared or distinctive among competing species. We contend that these predictions could successfully explain many of the complex and contradictory results of current research. We test some of these predictions based on long-term data for small mammals from the Chihuahuan Desert in the United States. and Mexico. We conclude that the landscape-of-fear model does provide reasonable explanations for many of the reported studies and should be tested further to better understand the effects of bottom-up, top-down, and parallel factors on population dynamics. C1 [Laundre, John W.; Hernandez, Lucina; Lopez-Portillo, Jorge; Gonzalez-Romero, Alberto; Grajales-Tam, Karina M.] Inst Ecol, AC, Xalapa 91070, Veracruz, Mexico. [Laundre, John W.; Hernandez, Lucina; Gronemeyer, Peg] New Mexico State Univ, Las Cruces, NM 88003 USA. [Laundre, John W.; Hernandez, Lucina] SUNY Coll Oswego, Oswego, NY 13126 USA. [Lopez Medina, Perla] Univ Autonoma Queretaro, Queretaro 76010, Qro, Mexico. [Campanella, Andrea] New Mexico State Univ, Jornada Basin LTER USDA ARS, Las Cruces, NM 88003 USA. [Browning, Dawn M.] USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. RP Laundre, JW (reprint author), Inst Ecol, AC, Xalapa 91070, Veracruz, Mexico. EM launjohn@hotmail.com RI Lopez Portillo, Jorge/L-2492-2014 OI Lopez Portillo, Jorge/0000-0002-6827-3297 FU CONACyT of Mexico [1843P-N9507]; Earthwatch Institute; INECOL [902-16]; SEMAR-NAT of Mexico; U.S. National Science Foundation [DEB-0004526]; New Mexico State University Agricultural Experiment Center; TE Inc.; American Society of Mammalogists; Ecological Society of America FX This work was conducted in conjunction with the Mapimi Long-Term Exclusion Experiment (MLTER) coordinated by L. Hernandez and supported by grants from CONACyT (1843P-N9507) of Mexico to Dr. Hernandez, Earthwatch Institute to Dr. Laundre, INECOL 902-16 to J. Lopez-Portillo, SEMAR-NAT of Mexico, and U.S. National Science Foundation (DEB-0004526); New Mexico State University Agricultural Experiment Center; T&E Inc. (Grant for Conservation Biology Research, 2006, 2007); American Society of Mammalogists (Grant in Aid for Research, 2006, 2008); Ecological Society of America (Forrest Shreve Grant, 2006) to Andrea Campanella. The Jornada Basin LTER produced the data sets for vegetation cover for the Jornada site. We also had logistical and infrastructure support from the Instituto de Ecologia, A.C., estacion de campo Laboratorio del Desierto, Reserva de la Biosfera Mapimi, and USDA Arid Land Research Program, Jornada Experimental Range. We thank the following persons who helped us in the field: A.J. Martinez, Institute of Ecology students, and the many Earthwatch volunteers. NR 64 TC 25 Z9 25 U1 19 U2 162 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD MAY PY 2014 VL 95 IS 5 BP 1141 EP 1152 DI 10.1890/13-1083.1 PG 12 WC Ecology SC Environmental Sciences & Ecology GA AI3DU UT WOS:000336740500004 PM 25000746 ER PT J AU Li, GF Serba, DD Saha, MC Bouton, JH Lanzatella, CL Tobias, CM AF Li, Guifen Serba, Desalegn D. Saha, Malay C. Bouton, Joseph H. Lanzatella, Christina L. Tobias, Christian M. TI Genetic Linkage Mapping and Transmission Ratio Distortion in a Three-Generation Four-Founder Population of Panicum virgatum (L.) SO G3-GENES GENOMES GENETICS LA English DT Article DE biomass; feedstock; biofuel; allopolyploid ID SHOWING SEGREGATION DISTORTION; MAXIMUM-LIKELIHOOD MODELS; NUCLEAR-DNA CONTENT; SWITCHGRASS POPULATIONS; MAPS; MARKERS; GENOME; SEQUENCE; CONSTRUCTION; MICROSATELLITES AB Switchgrass (Panicum virgatum L.), a warm season, C4, perennial grass, is one of the predominant grass species of the North American tall grass prairies. It is viewed as a high-potential bioenergy feedstock species because it can produce large amounts of lignocellulosic material with relatively few inputs. The objectives of this project were to develop an advanced switchgrass population and use it for the construction of genetic linkage maps and trait characterization. A three-generation, four-founder population was created and a total of 182 progeny of this advanced population were genotyped, including a mixture of self-pollinated and hybrid individuals. The female map integrated both subpopulations and covered 1629 cM of the switchgrass genome, with an average map length of 91 cM per linkage group. The male map of the hybrid progeny covered 1462 cM, with an average map length of 81 cM per linkage group. Average marker density of the female and male maps was 3.9 and 3.5 cM per marker interval, respectively. Based on the parental maps, the genome length of switchgrass was estimated to be 1776 cM and 1596 cM for the female map and male map, respectively. The proportion of the genome within 5 cM of a mapped locus was estimated to be 92% and 93% for the female map and male map, respectively. Thus, the linkage maps have covered most of the switchgrass genome. The assessment of marker transmission ratio distortion found that 26% of the genotyped markers were distorted from either 1:1 or 3:1 ratios expected for segregation of single dose markers in one or both parents, respectively. Several regions affected by transmission ratio distortion were found, with linkage groups Ib-m and VIIIa-f most affected. C1 [Li, Guifen; Serba, Desalegn D.; Saha, Malay C.; Bouton, Joseph H.] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA. [Lanzatella, Christina L.; Tobias, Christian M.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Tobias, CM (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM christian.tobias@ars.usda.gov RI Tobias, Christian/B-6602-2009 OI Tobias, Christian/0000-0002-7881-750X FU Department of Energy; United States Department of Agriculture FX Our sincere appreciation goes to Yuhong Tang and her group for genotyping and the greenhouse group of the Noble Foundation for greenhouse maintenance of the population. We are very thankful to Brian Motes and his group members for taking care of field experiments. This research was supported with funds provided by the Department of Energy and United States Department of Agriculture. The United States Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by United States Department of Agriculture, Agricultural Research Service, over other products and organizations not mentioned. NR 58 TC 5 Z9 5 U1 2 U2 16 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD MAY 1 PY 2014 VL 4 IS 5 BP 913 EP 923 DI 10.1534/g3.113.010165 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA AH9TA UT WOS:000336483900016 PM 24637352 ER PT J AU Monteiro, JP Wise, C Morine, MJ Teitel, C Pence, L Williams, A McCabe-Sellers, B Champagne, C Turner, J Shelby, B Ning, BT Oguntimein, J Taylor, L Toennessen, T Priami, C Beger, RD Bogle, M Kaput, J AF Monteiro, Jacqueline Pontes Wise, Carolyn Morine, Melissa J. Teitel, Candee Pence, Lisa Williams, Anna McCabe-Sellers, Beverly Champagne, Catherine Turner, Jerome Shelby, Beatrice Ning, Baitang Oguntimein, Joan Taylor, Lauren Toennessen, Terri Priami, Corrado Beger, Richard D. Bogle, Margaret Kaput, Jim TI Methylation potential associated with diet, genotype, protein, and metabolite levels in the Delta Obesity Vitamin Study SO GENES AND NUTRITION LA English DT Article DE Systems nutrition; Methylation potential; SAM/SAH; Micronutrients; Community-based participatory research ID LOWER MISSISSIPPI DELTA; ONE-CARBON METABOLISM; PERFORMANCE LIQUID-CHROMATOGRAPHY; POLYUNSATURATED FATTY-ACID; S-ADENOSYLMETHIONINE; ALZHEIMERS-DISEASE; GENETIC-VARIATION; PARTICIPATORY RESEARCH; NUTRIGENOMICS RESEARCH; PREDEMENTIA SYNDROMES AB Micronutrient research typically focuses on analyzing the effects of single or a few nutrients on health by analyzing a limited number of biomarkers. The observational study described here analyzed micronutrients, plasma proteins, dietary intakes, and genotype using a systems approach. Participants attended a community-based summer day program for 6-14 year old in 2 years. Genetic makeup, blood metabolite and protein levels, and dietary differences were measured in each individual. Twenty-four-hour dietary intakes, eight micronutrients (vitamins A, D, E, thiamin, folic acid, riboflavin, pyridoxal, and pyridoxine) and 3 one-carbon metabolites [homocysteine (Hcy), S-adenosylmethionine (SAM), and S-adenosylhomocysteine (SAH)], and 1,129 plasma proteins were analyzed as a function of diet at metabolite level, plasma protein level, age, and sex. Cluster analysis identified two groups differing in SAM/SAH and differing in dietary intake patterns indicating that SAM/SAH was a potential marker of nutritional status. The approach used to analyze genetic association with the SAM/SAH metabolites is called middle-out: SNPs in 275 genes involved in the one-carbon pathway (folate, pyridoxal/pyridoxine, thiamin) or were correlated with SAM/SAH (vitamin A, E, Hcy) were analyzed instead of the entire 1M SNP data set. This procedure identified 46 SNPs in 25 genes associated with SAM/SAH demonstrating a genetic contribution to the methylation potential. Individual plasma metabolites correlated with 99 plasma proteins. Fourteen proteins correlated with body mass index, 49 with group age, and 30 with sex. The analytical strategy described here identified subgroups for targeted nutritional interventions. C1 [Monteiro, Jacqueline Pontes] Univ Sao Paulo, Fac Nutr & Metab, Fac Med, Dept Pediat, Ribeirao Preto, SP, Brazil. [Wise, Carolyn; Teitel, Candee; Williams, Anna; Ning, Baitang; Toennessen, Terri] US FDA, Div Personalized Nutr & Med, Natl Ctr Toxicol Res, Jefferson, AR USA. [Morine, Melissa J.; Priami, Corrado] Univ Trento, Dept Math, Trento, Italy. [Morine, Melissa J.; Priami, Corrado] Univ Trento, Microsoft Res, Ctr Computat & Syst Biol COSBI, Rovereto, Italy. [Pence, Lisa; Beger, Richard D.] US FDA, Div Syst Biol, NCTR, Jefferson, AR USA. [McCabe-Sellers, Beverly; Bogle, Margaret] ARS, Delta Obes Prevent Res Unit, USDA, Little Rock, AR USA. [Champagne, Catherine] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Turner, Jerome; Shelby, Beatrice] Boys Girls Adults Community Dev Ctr & Phillips Ct, Marvell, AR USA. [Oguntimein, Joan; Taylor, Lauren] Washington & Lee Univ, Shepherd Program Interdisciplinary Study Poverty, Lexington, VA 24450 USA. [Oguntimein, Joan] Drexel Univ, Sch Med, Philadelphia, PA 19104 USA. [Taylor, Lauren] Emory Sch Publ Hlth, Atlanta, GA USA. [Kaput, Jim] Nestle Inst Hlth Sci, Syst Nutr & Hlth Unit, CH-1015 Lausanne, Switzerland. RP Kaput, J (reprint author), Nestle Inst Hlth Sci, Syst Nutr & Hlth Unit, Innovat Sq,EPFL Campus, CH-1015 Lausanne, Switzerland. EM james.kaput@rd.nestle.com RI Monteiro, Jacqueline/F-6987-2012 FU Division of Personalized Nutrition and Medicine at the U.S. FDA National Center for Toxicological Research (Jefferson AR); USDA Agricultural Research Service Delta Obesity Prevention Research Unit (Little Rock, AR); Nestle Institute of Health Sciences (Lausanne, Switzerland) FX The research team gratefully acknowledges the contributions of the Phillips County community members in the Marvell (AR) School District for their participation in this research. We also are indebted to the Helena Regional Medical Center Phlebotomists for their participation in this research. Members of the Divisions of Personalized Nutrition and Medicine and Systems Biology generously assisted with activities during the assessment periods. Lauren Taylor, Alyshia Phillips, and Lauren Shinholster of the Shepard Poverty Program were interns during the summer day camp. This research was funded by the Division of Personalized Nutrition and Medicine at the U.S. FDA National Center for Toxicological Research (Jefferson AR), the USDA Agricultural Research Service Delta Obesity Prevention Research Unit (Little Rock, AR), and the Nestle Institute of Health Sciences (Lausanne, Switzerland). NR 138 TC 8 Z9 10 U1 1 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1555-8932 EI 1865-3499 J9 GENES NUTR JI Genes Nutr. PD MAY PY 2014 VL 9 IS 3 AR 403 DI 10.1007/s12263-014-0403-9 PG 19 WC Genetics & Heredity; Nutrition & Dietetics SC Genetics & Heredity; Nutrition & Dietetics GA AH8MG UT WOS:000336391300015 PM 24760553 ER PT J AU Qu, GG Fetterer, R Leng, L Du, X Zarlenga, D Shen, ZQ Han, WY Bucala, R Tuo, WB AF Qu, Guanggang Fetterer, Raymond Leng, Lin Du, Xin Zarlenga, Dante Shen, Zhiqiang Han, Wenyu Bucala, Richard Tuo, Wenbin TI Ostertagia ostertagi macrophage migration inhibitory factor is present in all developmental stages and may cross-regulate host functions through interaction with the host receptor SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Article DE Ostertagia ostertagi; Macrophage migration inhibitory factor; Oos-MIF; Cattle; Nematode ID TAUTOMERASE ACTIVE-SITE; FACTOR MIF; PARASITIC NEMATODE; CRYSTAL-STRUCTURE; OLIGOMERIZATION STATE; TRICHINELLA-SPIRALIS; ENZYMATIC-ACTIVITY; GENE-EXPRESSION; CYTOKINE; CATTLE AB Macrophage migration inhibitory factor (MIF) of Ostertagia ostertagi, an abomasal parasite of cattle, was characterised in the present study. Phylogenetic analysis identified at least three O. ostertagi MIFs (Oos-MIFs), each encoded by a distinct transcript: Oos-MIF-1.1, Oos-MIF-1.2 and Oos-MIF-2. Oos-MIF-2 is only distantly related to Oos-MIF-1s, but has higher sequence similarity with the Caenorhabditis elegans MIF2. Oos-MIF-1.1 and Oos-MIF-1.2 are similar (93%) and thus collectively referred to as Oos-MIF-1 when characterised with immunoassays. Recombinant Oos-MIF-1.1 (rOos-MIF-1.1) is catalytically active as a tautomerase. A mutation (rOos-MIF-1.1P1G) or duplication of Prol residue (rOos-MIF-1.1P1+P) resulted in reduced oligomerisation and loss of tautomerase activity. The tautomerase activity of rOos-MIF-1.1 was only partially inhibited by ISO-1 but was abrogated by a rOos-MIF-1.1-specific antibody. Oos-MIF-1 was detected in all developmental stages of O. ostertagi, with higher levels in the adult stage; it was also detected in adult worm excretory/secretory product. Oos-MIF-1 was localised to the hypodermis/muscle, reproductive tract and intestine, but not to the cuticle. rOos-MIF-1.1, but not rOos-MIF-1.1P1G, was able to specifically bind to human CD74, a MIF cell surface receptor, with an affinity comparable with human MIF. Immunostaining indicated that macrophages were able to internalise rOos-MIF-1.1, further supporting receptor-mediated transportation. Herein we also show that rOos-MIF-1.1 inhibited migration of bovine macrophages and restored glucocorticoid-suppressed, lipopolysaccharide-induced TNF-alpha and IL-8 in human and/or bovine macrophages. Given its dual role in self-regulation and molecular mimicry, this secreted parasite protein warrants investigation as a vaccine candidate against O. ostertagi infections in cattle. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. C1 [Qu, Guanggang; Fetterer, Raymond; Zarlenga, Dante; Tuo, Wenbin] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Qu, Guanggang; Shen, Zhiqiang] Shangdong Binzhou Acad Anim Sci & Vet Med, Binzhou City 256600, Shandong, Peoples R China. [Leng, Lin; Du, Xin; Bucala, Richard] Yale Univ, Sch Med, Dept Med, New Haven, CT 06520 USA. [Han, Wenyu] Jilin Univ, Coll Vet Med & Anim Sci, Changchun 130023, Peoples R China. RP Tuo, WB (reprint author), ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. EM wenbin.tuo@ars.usda.gov FU United States Department of Agriculture Agriculture, Research Service (USDA/ARS), USA; National Institutes of Health (NIH, USA) [RO1AI042310] FX The authors wish to thank Mr. Eli Miramontea, Ms. Debora Hebert and Ms. Ruth Barfield for technical assistance. The authors wish to particularly thank Drs. Jitender Dubey and Oliver Kwok for assistance with immunohistochemistry and Dr. Benjamin Rosenthal for assistance with phylogenetic analysis. This research was supported by United States Department of Agriculture Agriculture, Research Service (USDA/ARS), USA in-house funding (WT) and National Institutes of Health (NIH, USA) RO1AI042310 (RB). NR 56 TC 3 Z9 3 U1 0 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 EI 1879-0135 J9 INT J PARASITOL JI Int. J. Parasit. PD MAY PY 2014 VL 44 IS 6 BP 355 EP 367 DI 10.1016/j.ijpara.2014.01.009 PG 13 WC Parasitology SC Parasitology GA AI2UA UT WOS:000336712400003 PM 24583184 ER PT J AU Sainju, UM Stevens, WB Caesar-TonThat, T Liebig, MA Wang, J AF Sainju, Upendra M. Stevens, William B. Caesar-TonThat, Thecan Liebig, Mark A. Wang, Jun TI Net Global Warming Potential and Greenhouse Gas Intensity Influenced by Irrigation, Tillage, Crop Rotation, and Nitrogen Fertilization SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CARBON-DIOXIDE EMISSION; NORTHERN GREAT-PLAINS; SOIL CARBON; LONG-TERM; AGRICULTURE; SEQUESTRATION; MANAGEMENT; FLUXES; OXIDE; SEQUENCE AB Little information exists about how global warming potential (GWP) is affected by management practices in agroecosystems. We evaluated the effects of irrigation, tillage, crop rotation, and N fertilization on net GWP and greenhouse gas intensity (GHGI or GWP per unit crop yield) calculated by soil respiration (GWP(R) and GHGI(R)) and organic C (SOC) (GWP(C) and GHGI(C)) methods after accounting for CO2 emissions from all sources (irrigation, farm operations, N fertilization, and greenhouse gas [GHG] fluxes) and sinks (crop residue and SOC) in a Lihen sandy loam from 2008 to 2011 in western North Dakota. Treatments were two irrigation practices (irrigated vs. nonirrigated) and five cropping systems (conventional-till malt barley [Hordeum vulgaris L.] with N fertilizer [CTBN], conventional-till malt barley with no N fertilizer [CTBO], no-till malt barley-pea [Pisum sativum L.] with N fertilizer [NTB-P], no-till malt barley with N fertilizer, and no-till malt barley with no N fertilizer [NTBO]). While CO2 equivalents were greater with irrigation, tillage, and N fertilization than without, N2O and CH4 fluxes were 2 to 218 kg CO2 eq. ha(-1) greater in nonirrigated NTBN and irrigated CTBN than in other treatments. Previous year's crop residue and C sequestration rate were 202 to 9316 kg CO2 eq. ha(-1) greater in irrigated NTB-P than in other treatments. Compared with other treatments, GWP(R) and GWP(C) were 160 to 9052 kg CO2 eq. ha(-1) lower in irrigated and nonirrigated NTB-P. Similarly, GHGI(R) and GHGI(C) were lower in nonirrigated NTB-P than in other treatments. Regardless of irrigation practices, NTB-P may lower net GHG emissions more than other treatments in the northern Great Plains. C1 [Sainju, Upendra M.; Stevens, William B.; Caesar-TonThat, Thecan] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Liebig, Mark A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Wang, Jun] NW Univ Xian, Coll Urban Sci, Xian 710069, Peoples R China. [Wang, Jun] NW Univ Xian, Coll Environm Sci, Xian 710069, Peoples R China. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. EM upendra.sainju@ars.usda.gov FU USDA-ARS GRACEnet project FX The authors thank Bryan Gebhard and Bill Iversen for management and irrigation of field plots and Joy Barsotti, Chris Russell, and Ethan Schaff for collecting gas, soil, and plant samples in the field and analyzing in the laboratory. This work was supported partly by the USDA-ARS GRACEnet project. NR 41 TC 9 Z9 11 U1 11 U2 92 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2014 VL 43 IS 3 BP 777 EP 788 DI 10.2134/jeq2013.10.0405 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA AH6XN UT WOS:000336275700001 PM 25602807 ER PT J AU Borchard, N Spokas, K Prost, K Siemens, J AF Borchard, Nils Spokas, Kurt Prost, Katharina Siemens, Jan TI Greenhouse Gas Production in Mixtures of Soil with Composted and Noncomposted Biochars Is Governed by Char-Associated Organic Compounds SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID POLYCYCLIC AROMATIC-HYDROCARBONS; NITROUS-OXIDE EMISSIONS; BLACK CARBON BIOCHAR; N2O EMISSIONS; METHANE OXIDATION; FARMYARD MANURE; IMPACT; CO2; DYNAMICS; METAANALYSIS AB Biochar application to soil has the potential to increase soil productivity while reducing anthropogenic greenhouse gas (GHG) emissions to the atmosphere. However, techniques for conditioning this material for maximizing its effects as a soil amendment require elucidation. We examined changes of organic matter associated with two biochars after 175 d of composting and the resulting effects on GHG emissions during a 150-d incubation period. Composting decreased the amount of organic compounds that could be thermally released from the biochars and affected their molecular nature. These thermally desorbable organic compounds from initial biochars likely stimulated the oxidation of CH4 and inhibited the production of N2O in soil-biochar mixtures. However, these reductions of GHG emissions disappeared together with thermally desorbable organic compounds after the composting of chars. Instead, addition of composted gasification coke and charcoal stimulated the formation of CH4 and increased N2O emissions by 45 to 56%. Nitrous oxide emissions equaled 20% of the total amount of N added with composted biochars, suggesting that organic compounds and N sorbed by the chars during composting fueled GHG production. The transient nature of the suppression of CH4 and N2O production challenges the long-term GHG mitigation potential of biochar in soil. C1 [Borchard, Nils; Prost, Katharina; Siemens, Jan] Univ Bonn, Inst Crop Sci & Resource Conservat, D-53115 Bonn, Germany. [Borchard, Nils] Forschungszentrum Julich, Agrosphere IBG 3, D-52425 Julich, Germany. [Spokas, Kurt] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. RP Borchard, N (reprint author), Univ Bonn, Inst Crop Sci & Resource Conservat, Nussallee 13, D-53115 Bonn, Germany. EM n.borchard@fz-juelich.de RI borchard, nils/J-7076-2012; Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 FU German Federal Institute for Geo-Science and Natural Resources FX This study was funded by the German Federal Institute for Geo-Science and Natural Resources. The authors thank the staff at the Wiesengut experimental station for support during the composting experiment; Dr. Timo Kautz, Johannes Siebigteroth, Adolf Kiener, Anke Lindecke, Martin du Saire, Michael Ottman, Edward Colosky, Eric Nooker, and Minh-Chi Tran-Thi for help with laboratory analyses and measurements; Mothermik GmbH for providing the gasification coke; and the reviewers for valuable suggestions for improving the manuscript. NR 73 TC 13 Z9 13 U1 5 U2 65 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2014 VL 43 IS 3 BP 971 EP 979 DI 10.2134/jeq2013.07.0290 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AH6XN UT WOS:000336275700020 PM 25602826 ER PT J AU Belofsky, G Aronica, M Foss, E Diamond, J Santana, F Darley, J Dowd, PF Coleman, CM Ferreira, D AF Belofsky, Gil Aronica, Mario Foss, Eric Diamond, Jane Santana, Felipe Darley, Jacob Dowd, Patrick F. Coleman, Christina M. Ferreira, Daneel TI Antimicrobial and Antiinsectan Phenolic Metabolites of Dalea searlsiae SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID RESISTANT STAPHYLOCOCCUS-AUREUS; FALL ARMYWORM; ABSOLUTE-CONFIGURATION; SPODOPTERA-FRUGIPERDA; CIRCULAR-DICHROISM; MILLETTIA DURA; FLAVONOIDS; ISOFLAVONOIDS; CHALCONES; FLAVANONE AB Continued interest in the chemistry of Dalea spp. led to investigation of Dalea searlsiae, a plant native to areas of the western United States. Methanol extractions of D. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans A-D (1-4) and known flavanones (5 and 6). Known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. Structure determination of pure compounds was accomplished primarily by extensive 1D- and 2D-NMR spectroscopy. The absolute configurations of compounds 1-5, 7, and 8 were assigned using electronic circular dichroism spectroscopy. Antimicrobial bioassays revealed significant activity concentrated in the plant roots. Compounds 1-6 exhibited MICs of 2-8 mu g/mL against Streptococcus mutans, Bacillus cereus, and oxacillin-sensitive and -resistant Staphylococcus aureus. Aerial metabolites 7-10 were inactive against these organisms, but the presence of 7 and 8 prompted investigation of the antiinsectan activity of D. searlsiae metabolites toward the major crop pest Spodoptera frugiperda (fall armyworm). While compounds 1-10 all caused significant reductions in larval growth rates, associated mortality (33-66%) was highest with flavanone 4 and rotenoids 7 and 8. These findings suggest a differential allocation of antimicrobial and antiinsectan plant resources to root and aerial portions of the plant, respectively. C1 [Belofsky, Gil; Aronica, Mario] Cent Washington Univ, Dept Chem, Ellensburg, WA 98926 USA. [Foss, Eric; Diamond, Jane; Santana, Felipe; Darley, Jacob] Cent Washington Univ, Dept Biol Sci, Ellensburg, WA 98926 USA. [Dowd, Patrick F.] ARS, Crop Bioprotect Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Coleman, Christina M.; Ferreira, Daneel] Univ Mississippi, Dept Pharmacognosy, University, MS 38677 USA. [Coleman, Christina M.; Ferreira, Daneel] Univ Mississippi, Pharmaceut Sci Res Inst, Sch Pharm, University, MS 38677 USA. RP Belofsky, G (reprint author), Cent Washington Univ, Dept Chem, Ellensburg, WA 98926 USA. EM belofskyg@cwu.edu FU NIH [S10-RR023384-01]; CWU, College of the Sciences Science Talent Expansion Program (STEP) FX The authors would like to thank the University of Iowa HRMS Facility, operating under its NIH shared instrument grant (S10-RR023384-01), for discounted services. We gratefully acknowledge the CWU, College of the Sciences Science Talent Expansion Program (STEP), for student financial support and for G.B.'s start-up funding. Special thanks to Prof. C. Shock, OSU, Malheur Experiment Station, for providing plant material and water content measurement, and Dr. W. Massefski, Mass Analytical, for assistance with in-house 2D-NMR experimental setup and troubleshooting. We also thank Dr. D. Rosado and the Department of Chemistry and Biochemistry, Mississippi College, Clinton, MS, for the use of the JASCO J-815 CD spectrometer. NR 57 TC 6 Z9 6 U1 1 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD MAY PY 2014 VL 77 IS 5 BP 1140 EP 1149 DI 10.1021/np401083g PG 10 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA AI0BH UT WOS:000336510700008 PM 24761805 ER PT J AU Fielding, RA AF Fielding, R. A. TI Frailty, identification, treatment, and clinical practice SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Editorial Material ID PHENOTYPE C1 Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 EI 1760-4788 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD MAY PY 2014 VL 18 IS 5 BP 469 EP 469 DI 10.1007/s12603-014-0454-z PG 1 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA AI1KI UT WOS:000336609400006 PM 24886731 ER PT J AU Bischoff-Ferrari, HA Orav, EJ Willett, WC Dawson-Hughes, B AF Bischoff-Ferrari, Heike A. Orav, Endel J. Willett, Walter C. Dawson-Hughes, Bess TI The effect of vitamin D supplementation on skeletal, vascular, or cancer outcomes SO LANCET DIABETES & ENDOCRINOLOGY LA English DT Letter ID PLACEBO-CONTROLLED TRIAL C1 [Bischoff-Ferrari, Heike A.] Univ Zurich, Dept Geriatr & Aging Res, Zurich, Switzerland. [Orav, Endel J.] Harvard Univ, Sch Med, Dept Med, Cambridge, MA 02138 USA. [Willett, Walter C.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Cambridge, MA 02138 USA. [Dawson-Hughes, Bess] Tufts Univ Boston, Jean Mayer USDA Human Nutr Res Ctr Aging, Medford, MA 02155 USA. RP Bischoff-Ferrari, HA (reprint author), Univ Zurich, Dept Geriatr & Aging Res, Zurich, Switzerland. EM heikeabischoff@aol.com NR 3 TC 5 Z9 5 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2213-8587 J9 LANCET DIABETES ENDO JI Lancet Diabetes Endocrinol. PD MAY PY 2014 VL 2 IS 5 BP 363 EP 364 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AI2XZ UT WOS:000336723800013 PM 24795248 ER PT J AU Childers, DL Pickett, STA Grove, JM Ogden, L Whitmer, A AF Childers, Daniel L. Pickett, Steward T. A. Grove, J. Morgan Ogden, Laura Whitmer, Alison TI Advancing urban sustainability theory and action: Challenges and opportunities SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Urban sustainability; Sanitary city; Transitions; Inertia; Sustainable city; Ecology for cities ID SOCIAL-ECOLOGICAL SYSTEMS; EARTH STEWARDSHIP; DEVELOPING-WORLD; LONG-TERM; LAND-USE; CITIES; DESIGN; RESILIENCE; CRISIS; SCIENCE AB Urban ecology and its theories are increasingly poised to contribute to urban sustainability, through both basic understanding and action. We present a conceptual framework that expands the Industrial : Sanitary Sustainable City transition to include non-sanitary cities, "new cities", and various permutations of transition options for cities encountering exogenous and endogenous "triggers of change". When investigating and modeling these urban transitions, we should consider: (1) the triggers that have induced change: (2) situations where crisis triggers change; (3) why cities transition toward more sustainable states on their own, in the absence of crisis; (4) what we can learn from new city transitions, and non-sanitary city transitions; and (5) how resource interactions affect urban transition s. Several existing theoretical frameworks, including sustainability, resilience, adaptation, and vulnerability, may be helpful when considering urban transitions. We suggest that all of these theories interact through inertia in urban systems, and that this multi-faceted inertia e.g. institutional inertia, infrastructural inertia, and social inertia imparts degrees of rigidity that make urban systems less flexible and nimble when facing transitional triggers and change. Given this, solutions to urban sustainability challenges may be categorized as those: (1) that "tweak" the current systems and work with or even take advantage of the inertia in those systems, versus; (2) that are more "transformative", that confront systemic inertia, and that may require new systems. We propose that a model for addressing urban sustainability in the context of relevant theory, and for bridging research and practice, should focus on intercity comparisons. And one mechanism to facilitate this approach is a newly formed interdisciplinary Research Coordination Network (RCN) that focuses on urban sustainability by integrating urban research while incubating solutions-oriented products and collaborative partnerships with practitioners. The Network includes more than two dozen cities in five continents that are in various degrees of transition. In the true vein of sustainability science, our Network activities are incubating societally-relevant solutions through projects that will lead to tangible, "on the ground" sustainable solutions for all types of cities. Our ultimate goal is to understand the process by which cities become more sustainable while affecting that process through action inspired by knowledge. (C) 2014 Elsevier B.V. All rights reserved. C1 [Childers, Daniel L.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [Pickett, Steward T. A.] Cary Inst Ecosyst Studies, Millbrook, NY USA. [Grove, J. Morgan] US Forest Serv, Baltimore, MD USA. [Ogden, Laura] Florida Int Univ, Miami, FL 33199 USA. [Whitmer, Alison] Georgetown Univ, Washington, DC USA. RP Childers, DL (reprint author), Arizona State Univ, Sch Sustainabil, 800 South Cady Mall,Wrigley Hall, Tempe, AZ 85287 USA. EM dan.childers@asu.edu FU U.S. National Science Foundation (NSF) [1140070]; NSF [1026865, 1027188, 0948988] FX These activities and the Urban Sustainability Research Coordination Network we describe here is supported by the U.S. National Science Foundation (NSF) through Grant No. 1140070. Additional support has been provided by the NSF to D.L.C. through the CAP LTER Program (Grant No. 1026865), to S.T.A.P. and J.M.G. through the BES LTER Program (Grant No. 1027188), and to L. Miami-Dade ULTRA-Ex Program (Grant No. 0948988). We thank 3 anonymous reviewers for their helpful comments with the manuscript. NR 96 TC 32 Z9 33 U1 6 U2 105 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAY PY 2014 VL 125 SI SI BP 320 EP 328 DI 10.1016/j.landurbplan.2014.01.022 PG 9 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AH9ME UT WOS:000336465700032 ER PT J AU Spartano, NL Lamon-Fava, S Matthan, NR Obin, MS Greenberg, AS Lichtenstein, AH AF Spartano, Nicole L. Lamon-Fava, Stefania Matthan, Nirupa R. Obin, Martin S. Greenberg, Andrew S. Lichtenstein, Alice H. TI Linoleic Acid Suppresses Cholesterol Efflux and ATP-Binding Cassette Transporters in Murine Bone Marrow-Derived Macrophages SO LIPIDS LA English DT Article DE Bone marrow-derived macrophages; Cholesterol efflux; ATP-binding cassette transporters; Sterol regulatory element binding protein; Atherosclerosis; Unsaturated fatty acids ID TYPE-2 DIABETES-MELLITUS; FOAM CELL-FORMATION; FATTY-ACIDS; RESPONSIVE ELEMENT; DESTABILIZE ABCA1; EXPRESSION; ABCG1; G1; GENE; PHOSPHORYLATE AB Individuals with type 2 diabetes mellitus (T2DM) are at increased risk of developing cardiovascular disease (CVD), possibly associated with elevated plasma free fatty acid concentrations. Paradoxically, evidence suggests that unsaturated, compared to saturated fatty acids, suppress macrophage cholesterol efflux, favoring cholesterol accumulation in the artery wall. Murine bone marrow-derived macrophages (BMDM) were used to further explore the relationship between saturated and unsaturated fatty acids, and cholesterol efflux mediated by ATP-binding cassette transporters (ABCA1 and ABCG1) through transcription factors liver-x-receptor-alpha (LXR-alpha) and sterol receptor element binding protein (SREBP)-1. BMDM isolated from C57BL/6 mice were exposed to 100 mu M linoleic acid (18:2) or palmitic acid (16:0) for 16 h, and 25 mu g/mL oxidized low density lipoprotein for an additional 24 h. ABCA1 and ABCG1 mRNA expression was suppressed to a greater extent by 18:2 (60 % and 54 %, respectively) than 16:0 (30 % and 29 %, respectively) relative to the control (all p < 0.01). 18:2 decreased ABCA1 protein levels by 94 % and high density lipoprotein (HDL) mediated cholesterol efflux by 53 % (both p < 0.05), and had no significant effect on ABCG1, LXR-alpha or SREBP-1 protein levels. 16: 0 had no effect on ABCA1, ABCG1, LXR-alpha or SREBP-1 protein expression or HDL-mediated cholesterol efflux. These results suggest that 18:2, relative to 16:0, attenuated macrophage HDL-mediated cholesterol efflux through down regulation of ABCA1 mRNA and protein levels but not through changes in LXR-alpha or SREBP-1 expression. The effect of 18:2 relative to 16:0 on macrophages cholesterol homeostasis may exacerbate the predisposition of individuals with T2DM to increased CVD risk. C1 [Spartano, Nicole L.; Lamon-Fava, Stefania; Matthan, Nirupa R.; Obin, Martin S.; Greenberg, Andrew S.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Cardiovasc Nutr Lab, JM USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM nicole.spartano@tufts.edu; stefania.lamon-fava@tufts.edu; nirupa.matthan@tufts.edu; martin.obin@tufts.edu; andrew.greenberg@tufts.edu; alice.lichtenstein@tufts.edu FU NIH [NHLBI-T32-HL069772]; USDA [58-1950-0-0014] FX The authors would like to thank Dr. Donald Smith and staff from the Comparative Biology Unit at the Jean Mayer USDA Human Nutrition Research Center on Aging (Tufts University, Boston) for assistance with animal care and feeding. This work was supported by grants from the NIH: NHLBI-T32-HL069772 (NLS) and the USDA agreement No. 58-1950-0-0014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 32 TC 0 Z9 0 U1 0 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD MAY PY 2014 VL 49 IS 5 BP 415 EP 422 DI 10.1007/s11745-014-3890-y PG 8 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA AI3ZY UT WOS:000336805900002 PM 24595513 ER PT J AU Coffman, JM Bestelmeyer, BT Kelly, JF Wright, TF Schooley, RL AF Coffman, John M. Bestelmeyer, Brandon T. Kelly, Jeffrey F. Wright, Timothy F. Schooley, Robert L. TI Restoration Practices Have Positive Effects on Breeding Bird Species of Concern in the Chihuahuan Desert SO RESTORATION ECOLOGY LA English DT Article DE avian community analysis; grassland restoration; Peucaea cassinii; Polioptila melanura; rangeland management; shrub encroachment ID COMMUNITY STRUCTURE; SHRUB ENCROACHMENT; GRASSLAND BIRDS; NEW-MEXICO; VEGETATION; HABITATS; CONSERVATION; ASSEMBLAGES; DYNAMICS; IMPACTS AB Woody plant encroachment into grasslands is a global concern. Efforts to restore grasslands often assume that removal of woody plants benefits biodiversity but assumptions are rarely tested. In the Chihuahuan Desert of the Southwestern United States, we tested whether abundances of grassland specialist bird species would be greater in plant communities resulting from treatment with herbicides to remove encroaching shrubs compared with untreated shrub-dominated areas that represented pre-treatment conditions. In 2010, we surveyed breeding birds and vegetation at 16 treated-untreated pairs. In 2011, we expanded the survey effort to 21 treated-untreated pairs, seven unpaired treatment areas, and five reference grassland areas. Vegetation in treatment areas had higher perennial grass foliar and basal cover and lower shrub foliar cover compared with untreated areas. Several regionally declining grassland specialists exhibited higher occurrence and relative abundance in treated areas. A shrubland specialist, however, was associated with untreated areas and may be negatively impacted by shrub removal. Bird community composition differed between treated and untreated areas in both years. Our results indicate that shrub removal can have positive effects on grassland specialist bird species, but that a mosaic of treated and untreated areas might be most beneficial for regional biodiversity. C1 [Coffman, John M.; Wright, Timothy F.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Coffman, John M.; Bestelmeyer, Brandon T.] New Mexico State Univ, USDA ARS, Jornada Expt Range & Jornada Basin LTER, Las Cruces, NM 88003 USA. [Kelly, Jeffrey F.] Univ Oklahoma, Oklahoma Biol Survey, Norman, OK 73019 USA. [Kelly, Jeffrey F.] Univ Oklahoma, Ecol & Evolutionary Biol Program, Dept Biol, Norman, OK 73019 USA. [Schooley, Robert L.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. RP Coffman, JM (reprint author), New Mexico State Univ, Dept Biol, MSC 3AF, Las Cruces, NM 88003 USA. EM johncoffman@gmail.com RI Kelly, Jeffrey/B-2029-2015 OI Kelly, Jeffrey/0000-0002-8255-7990 FU USDA-AFRI Managed Ecosystems Program; BLM-LCDO; New Mexico Ornithological Society (Ryan Beaulieu Research Grant); USDA-ARS Jornada Experimental Range FX This research was supported by a grant from the USDA-AFRI Managed Ecosystems Program, BLM-LCDO, and New Mexico Ornithological Society (Ryan Beaulieu Research Grant), and appropriated funds to USDA-ARS Jornada Experimental Range. We gratefully acknowledge L. Burkett and the U. S. Bureau of Land Management, particularly Ray Lister, for logistical support. We would like to thank D. Griffin, K. Sierzega, E. Tsentas, and T. Rogers for assistance in the field. L. Burkett provided helpful comments on the manuscript. NR 46 TC 7 Z9 7 U1 2 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAY PY 2014 VL 22 IS 3 BP 336 EP 344 DI 10.1111/rec.12081 PG 9 WC Ecology SC Environmental Sciences & Ecology GA AI4NQ UT WOS:000336842500012 ER PT J AU Cheek, MD Williams, GE Bousquin, SG Colee, J Melvin, SL AF Cheek, Michael D. Williams, Gary E. Bousquin, Stephen G. Colee, James Melvin, Stefani L. TI Interim Response of Wading Birds (Pelecaniformes and Ciconiiformes) and Waterfowl (Anseriformes) totheKissimmee River Restoration Project, Florida, USA SO RESTORATION ECOLOGY LA English DT Article DE Florida; monitoring; performance measure; restoration effect; wading birds; waterfowl; wetland restoration evaluation; wildlife metrics ID PREY AVAILABILITY; AERIAL SURVEY; EVERGLADES; INDICATORS; WATERBIRDS; SELECTION; ECOSYSTEM; WETLANDS; HABITAT; SUCCESS AB Success of the Kissimmee River Restoration Project will be evaluated in part by monitoring populations of wading birds (Pelecaniformes and Ciconiiformes) and waterfowl (Anseriformes). These two waterbird guilds were integral components of the pre-channelization river-floodplain ecosystem, and both declined substantially following channelization. Restoration is expected to attract wading birds and waterfowl by reintroducing naturally fluctuating water levels, seasonal hydroperiods, and historic vegetation communities. Post-construction aerial surveys (November 2001 to May 2008) within the Phase I restoration area indicate that the abundance and species richness of both wading birds and waterfowl have shown a positive restoration response thus far. Dry season abundance of aquatic wading birds and waterfowl has exceeded restoration expectations (30.6birds/km2 and 3.9birds/km2, respectively) each year since the completion of restoration Phase I in 2001. While there has been a significant positive restoration effect on waterfowl abundance, waterfowl species richness (n=6) has not yet reached the restoration expectation of 13 species. Abundance of the terrestrial cattle egret (Bubulcus ibis), which increased dramatically after the majority of floodplain wetlands were converted to cattle pastures in the channelized system, has shown a significant negative response to restoration. It is anticipated that completion of the remaining phases of restoration (II/III), and implementation of the Kissimmee River Headwaters Revitalization water regulation schedule by 2019, will further increase and improve habitat for wading birds and waterfowl by reestablishing floodplain hydrology that more closely mimics historical conditions. C1 [Cheek, Michael D.; Bousquin, Stephen G.] Appl Sci Bur, Restorat Evaluat Unit, W Palm Beach, FL 33406 USA. [Williams, Gary E.] Nat Syst & Restorat Bur, Springs & Environm Flows Sect, Brooksville, FL 34604 USA. [Colee, James] Univ Florida, Inst Food & Agr Sci, Dept Stat, Gainesville, FL 32611 USA. [Melvin, Stefani L.] US Forest Serv, USDA, Salmon, ID 83467 USA. RP Cheek, MD (reprint author), Appl Sci Bur, Restorat Evaluat Unit, 3301 Gun Club Rd, W Palm Beach, FL 33406 USA. EM mcheek@sfwmd.gov NR 42 TC 4 Z9 4 U1 4 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD MAY PY 2014 VL 22 IS 3 BP 426 EP 434 DI 10.1111/rec.12059 PG 9 WC Ecology SC Environmental Sciences & Ecology GA AI4NQ UT WOS:000336842500021 ER PT J AU Liu, SB Yang, XP Zhang, DD Bai, GH Chao, SM Bockus, W AF Liu, Shubing Yang, Xiping Zhang, Dadong Bai, Guihua Chao, Shiaoman Bockus, William TI Genome-wide association analysis identified SNPs closely linked to a gene resistant to Soil-borne wheat mosaic virus SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; WHITE WINTER-WHEAT; BREAD WHEAT; POPULATION-STRUCTURE; LINKAGE ANALYSIS; SOILBORNE; FUROVIRUS; LANDRACES; DIVERSITY AB Key message Using association and linkage mapping, two SNP markers closely linked to the SBWMV resistance gene on chromosome 5D were identified and can be used to select the gene in breeding. Abstract Soil-borne wheat mosaic virus (SBWMV) disease is a serious viral disease of winter wheat growing areas worldwide. SBWMV infection can significantly reduce grain yield up to 80 %. Developing resistant wheat cultivars is the only feasible strategy to reduce the losses. In this study, wheat Infinium iSelect Beadchips with 9 K wheat SNPs were used to genotype an association mapping population of 205 wheat accessions. Six new SNPs from two genes were identified to be significantly associated with the gene for SBWMV resistance on chromosome 5D. The SNPs and Xgwm469, an SSR marker that has been reported to be associated with the gene, were mapped close to the gene using F6-derived recombinant inbred lines from the cross between a resistant parent 'Heyne' and a susceptible parent 'Trego'. Two representative SNPs, wsnp_CAP11_c209_198467 and wsnp_JD_c4438_5568170, from the two linked genes in wheat were converted into KBioscience Competitive Allele-Specific Polymerase assays and can be easily used in marker-assisted selection to improve wheat resistance to SBWMV in breeding. C1 [Liu, Shubing; Yang, Xiping; Zhang, Dadong; Bai, Guihua] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res Unit, Fargo, ND USA. [Bockus, William] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Bai, GH (reprint author), USDA ARS, Hard Winter Wheat Genet Res Unit, 4008 Throckmorton Hall, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This is contribution number 14-056-J from the Kansas Agricultural Experiment Station, Manhattan, KS, USA. This project is partly funded by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 53 TC 5 Z9 8 U1 2 U2 38 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2014 VL 127 IS 5 BP 1039 EP 1047 DI 10.1007/s00122-014-2277-z PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AI3IO UT WOS:000336754700003 PM 24522724 ER PT J AU Zhu, J Pearce, S Burke, A See, DR Skinner, DZ Dubcovsky, J Garland-Campbell, K AF Zhu, Jie Pearce, Stephen Burke, Adrienne See, Deven Robert Skinner, Daniel Z. Dubcovsky, Jorge Garland-Campbell, Kimberly TI Copy number and haplotype variation at the VRN-A1 and central FR-A2 loci are associated with frost tolerance in hexaploid wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID LOW-TEMPERATURE TOLERANCE; RESPONSIVE GENE-EXPRESSION; NEAR-ISOGENIC LINES; WILD EMMER WHEAT; FREEZING TOLERANCE; POLYPLOID WHEAT; WINTER-WHEAT; CBF GENES; CHROMOSOME 5A; TRANSCRIPTIONAL ACTIVATOR AB Key message The interaction between VRN-A1 and FR-A2 largely affect the frost tolerance of hexaploid wheat. Abstract Frost tolerance is critical for wheat survival during cold winters. Natural variation for this trait is mainly associated with allelic differences at the VERNALIZATION 1 (VRN1) and FROST RESISTANCE 2 (FR2) loci. VRN1 regulates the transition between vegetative and reproductive stages and FR2, a locus including several tandemly duplicated C-REPEAT BINDING FACTOR (CBF) transcription factors, regulates the expression of Cold-regulated genes. We identified sequence and copy number variation at these two loci among winter and spring wheat varieties and characterized their association with frost tolerance. We identified two FR-A2 haplotypes-'FR-A2-S' and 'FR-A2-T'-distinguished by two insertion/deletions and ten single nucleotide polymorphisms within the CBF-A12 and CBF-A15 genes. Increased copy number of CBF-A14 was frequently associated with the FR-A2-T haplotype and with higher CBF14 transcript levels in response to cold. Factorial ANOVAs revealed significant interactions between VRN1 and FR-A2 for frost tolerance in both winter and spring panels suggesting a crosstalk between vernalization and cold acclimation pathways. The model including these two loci and their interaction explained 32.0 and 20.7 % of the variation in frost tolerance in the winter and spring panels, respectively. The interaction was validated in a winter wheat F-4:5 population segregating for both genes. Increased VRN-A1 copy number was associated with improved frost tolerance among varieties carrying the FR-A2-T allele but not among those carrying the FR-A2-S allele. These results suggest that selection of varieties carrying the FR-A2-T allele and three copies of the recessive vrn-A1 allele would be a good strategy to improve frost tolerance in wheat. C1 [Zhu, Jie; Skinner, Daniel Z.; Garland-Campbell, Kimberly] Washington State Univ, Pullman, WA 99164 USA. [Pearce, Stephen; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Burke, Adrienne; See, Deven Robert; Skinner, Daniel Z.; Garland-Campbell, Kimberly] USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, USDA, Pullman, WA 99164 USA. [See, Deven Robert] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 28015 USA. [Dubcovsky, Jorge] Gordon & Betty Moore Fdn, Chevy Chase, MD 28015 USA. RP Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM jdubcovsky@ucdavis.edu; kgcamp@wsu.edu RI Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU National Research Initiative Competitive Grants from the USDA National Institute of Food and Agriculture [WNR-2008-01010, 2011-68002-30029]; Washington Grains Commission Project [5345]; USDA, ARS [CWU: 5348-21220-003-00D]; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation FX This project was supported by the National Research Initiative Competitive Grants WNR-2008-01010 and 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture, the Washington Grains Commission Project No. 5345, and USDA, ARS (in house) CWU: 5348-21220-003-00D. JD also acknowledges support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. NR 52 TC 19 Z9 21 U1 4 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2014 VL 127 IS 5 BP 1183 EP 1197 DI 10.1007/s00122-014-2290-2 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AI3IO UT WOS:000336754700014 PM 24626953 ER PT J AU Kim, KS Chirumamilla, A Hill, CB Hartman, GL Diers, BW AF Kim, Ki-Seung Chirumamilla, Anitha Hill, Curtis B. Hartman, Glen L. Diers, Brian W. TI Identification and molecular mapping of two soybean aphid resistance genes in soybean PI 587732 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID HOMOPTERA; MAP AB Soybean [Glycine max (L.) Merr.] continues to be plagued by the soybean aphid (Aphis glycines Matsumura: SA) in North America. New soybean resistance sources are needed to combat the four identified SA biotypes. The objectives of this study were to determine the inheritance of SA resistance in PI 587732 and to map resistance gene(s). For this study, 323 F-2 and 214 F-3 plants developed from crossing PI 587732 to two susceptible genotypes were challenged with three SA biotypes and evaluated with genetic markers. Choice tests showed that resistance to SA Biotype 1 in the first F-2 population was controlled by a gene in the Rag1 region on chromosome 7, while resistance to SA Biotype 2 in the second population was controlled by a gene in the Rag2 region on chromosome 13. When 134 F-3 plants segregating in both the Rag1 and Rag2 regions were tested with a 1:1 mixture of SA Biotypes 1 and 2, the Rag2 region and an interaction between the Rag1 and Rag2 regions were significantly associated with the resistance. Based on the results of the non-choice tests, the resistance gene in the Rag1 region in PI 587732 may be a different allele or gene from Rag1 from Dowling because the PI 587732 gene showed antibiosis type resistance to SA Biotype 2 while Rag1 from Dowling did not. The two SA resistance loci and genetic marker information from this study will be useful in increasing diversity of SA resistance sources and marker-assisted selection for soybean breeding programs. C1 [Kim, Ki-Seung; Chirumamilla, Anitha; Hill, Curtis B.; Hartman, Glen L.; Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, Glen L.] Univ Illinois, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. RP Diers, BW (reprint author), Univ Illinois, Dept Crop Sci, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM bdiers@illinois.edu FU United Soybean Board; Illinois Soybean Association FX We thank the United Soybean Board and the Illinois Soybean Association for partial support of this research. NR 37 TC 3 Z9 4 U1 1 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2014 VL 127 IS 5 BP 1251 EP 1259 DI 10.1007/s00122-014-2296-9 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AI3IO UT WOS:000336754700019 PM 24705575 ER PT J AU Baker, JT Gitz, DC Payton, P Broughton, KJ Bange, MP Lascano, RJ AF Baker, Jeffrey T. Gitz, Dennis C., III Payton, Paxton Broughton, Katrina J. Bange, Michael P. Lascano, Robert J. TI Carbon Dioxide Control in an Open System that Measures Canopy Gas Exchanges SO AGRONOMY JOURNAL LA English DT Article ID ELEVATED ATMOSPHERIC CO2; RICE ORYZA-SATIVA; INTRASPECIFIC VARIATION; YIELD; CULTIVARS; CHAMBER; TEMPERATURE; ENRICHMENT; AMBIENT; FLUXES AB Atmospheric carbon dioxide concentration ([CO2]) affects both C-3 carbon net assimilation (A) as well as crop water use. Methods for measuring whole canopy gas exchange responses under [CO2] enrichment are needed for breeding programs aiming to develop crop cultivars resistant to stresses like drought in a future higher CO2 world. Previously we developed and tested a portable, open transparent chamber system for measuring canopy gas exchanges. Here we describe further development of this system by adding the capability of controlling [CO2]. Pure CO2 injection into the system was accomplished with a data logger operated mass flow controller attached to a high pressure CO2 gas cylinder. Across the full range of chamber air flow rates, [CO2] enrichment controls were within +/- 12 mu mol mol(-1) of the desired set point. Following an abrupt user-selected change in chamber air flow rate, [CO2] enrichment controls were re-established within 3 to 5 min. C1 [Baker, Jeffrey T.] ARS, USDA, Plant Stress & Water Conservat Lab, Big Spring, TX 79720 USA. [Gitz, Dennis C., III; Payton, Paxton; Lascano, Robert J.] ARS, USDA, Plant Stress & Water Conservat Lab, Lubbock, TX 79415 USA. [Broughton, Katrina J.; Bange, Michael P.] CSIRO, Plant Ind, Narrabri, NSW 2390, Australia. RP Baker, JT (reprint author), ARS, USDA, Plant Stress & Water Conservat Lab, 302 West I-20, Big Spring, TX 79720 USA. EM Jeff.Baker@ars.usda.gov RI Bange, Michael/A-1231-2008 FU Ogallala Aquifer Program; USDA Agricultural Research Service; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX The technical support of Charles Yates is gratefully acknowledged. This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 20 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 789 EP 792 DI 10.2134/agronj13.0450 PG 4 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300002 ER PT J AU Allen, BL Lenssen, AW Sainju, UM Caesar-TonThat, T Evans, RG AF Allen, B. L. Lenssen, A. W. Sainju, U. M. Caesar-TonThat, T. Evans, R. G. TI Nitrogen Use in Durum and Selected Brassicaceae Oilseeds in Two-Year Rotations SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; LENTIL GREEN-MANURE; WATER-USE; CANOLA PRODUCTION; USE EFFICIENCY; SPRING WHEAT; SOIL-WATER; NO-TILL; YIELD; FALLOW AB Brassicaceae oilseeds can serve as potential feedstocks for renewable biofuels to offset demand for petroleum-based alternatives. However, little is known about oilseed crop yield potential and N use in semiarid, wheat (Triticum spp.)-based cropping systems that dominate the northern Great Plains (NGP). A 5-yr study was conducted in northeast Montana to investigate the yield potential of a direct seeded system of durum (T. durum Desf.) in rotation with either chemical fallow or three Brassicaceae oilseeds: camelina [Camelina sativa (L.) Crantz], crambe (Crambe abyssinica Hochst. ex R. E. Fries), and canola-quality Brassica juncea L. Overall, results from the study indicated that seed yield in the three Brassicaceae oilseeds tested in rotation with durum was related (P < 0.001; r(2) = 0.68) to a nitrogen recovery index (NRI), indicating the importance of nitrogen use (NU) efficiency in dryland oilseed production, and that B. juncea generally used N more efficiently than crambe and camelina. Similarly, NRI was related (P < 0.001; r(2) = 0.72) to grain yield in durum following oilseeds. Grain yield of durum following B. juncea was similar to durum following fallow and greater than durum following camelina or crambe. Durum following crambe tended to use N more inefficiently than durum following camelina, B. juncea, or fallow. Differences in yield and N use of durum and oilseeds varied among years, which underscores the need to further develop management tools to optimize durum-oilseed cropping systems in highly variable rainfall environments typical of the NGP. C1 [Allen, B. L.; Sainju, U. M.; Caesar-TonThat, T.; Evans, R. G.] ARS, USDA, Sidney, MT 59270 USA. [Lenssen, A. W.] Iowa State Univ, Ames, IA 50011 USA. RP Allen, BL (reprint author), ARS, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. EM brett.allen@ars.usda.gov NR 37 TC 2 Z9 2 U1 1 U2 15 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 821 EP 830 DI 10.2134/agronj13.0158 PG 10 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300006 ER PT J AU Grimshaw, AL Schwartz, BM Grey, TL McCullough, PE Raymer, PL Webster, TM Kowalewski, AR Tate, TM Parrott, WA AF Grimshaw, Austin L. Schwartz, Brian M. Grey, Timothy L. McCullough, Patrick E. Raymer, Paul L. Webster, Theodore M. Kowalewski, A. R. Tate, Trent M. Parrott, Wayne A. TI Acetyl-CoA Carboxylase Herbicide Tolerance in Bermudagrass SO AGRONOMY JOURNAL LA English DT Article ID COMMON BERMUDAGRASS; POSTEMERGENCE; RESISTANCE; CROPS AB Contamination of newly planted bermudagrass (Cynodon spp.) varieties by undesirable off-type bermudagrass genotypes is an ever increasing concern for turf managers because selective control options are limited. In 2009, a sethoxydim {2-[1-(ethoxyimino) butyl]-5-[2-(ethylthio) propyl]-3-hydroxy-2-cyclohexen-1-one} tolerant bermudagrass genotype (93-175) was identified during herbicide screening at the University of Georgia in Tifton. The objective of this research was to assess the tolerance of 93-175 to three Acetyl-CoA carboxylase (ACCase) herbicides in comparison to the susceptible genotypes Tifway and common bermudagrass. Greenhouse and field trials were performed between August 2011 and April 2013. Factors in the field experiment included ACCase herbicides, application rates, bermudagrass genotypes, and locations. Turfgrass injury ratings taken 42 days after treatment (DAT) and during greenup the following spring supported initial preliminary findings. At the 1x rate of sethoxydim (280 g a. i. ha(-1)), 93-175 displayed 50 to 87% less injury in comparison to the susceptible genotypes. In the spring of 2013, 93-175 plots treated with a 1x rate of sethoxydim reached 100% recovery during the same time period as non-treated controls, while common and Tifway had only recovered to 48 and 60%, respectively. The tolerance mechanism of 93-175 to sethoxydim did not confer an appreciable reduction of clethodim {(E, E)-(6)-2-[1-[[(3-chloro-2-propenyl)oxy]imino]propyl]-5-[2-(ethylthio) propyl]-3-hydroxy-2-cyclohexen-1-one} or fluazifop {(6)-2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoic acid} herbicide treatment effects. 93-175 will continue to be studied to determine transferability of herbicide tolerance to progeny and the mechanism of the observed tolerance. C1 [Grimshaw, Austin L.; Schwartz, Brian M.; Grey, Timothy L.] Univ Georgia, Dep Crop & Soil Sci, Tifton, GA 31793 USA. [McCullough, Patrick E.; Raymer, Paul L.] Univ Georgia, Dep Crop & Soil Sci, Griffin, GA 30223 USA. [Webster, Theodore M.] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA 31794 USA. [Kowalewski, A. R.] Oregon State Univ, Dep Hort, Corvallis, OR 97331 USA. [Tate, Trent M.] Rutgers State Univ, Dep Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Parrott, Wayne A.] Univ Georgia, Dep Crop & Soil Sci, Athens, GA 30602 USA. RP Schwartz, BM (reprint author), Univ Georgia, Dep Crop & Soil Sci, Tifton, GA 31793 USA. EM tifturf@uga.edu RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 NR 27 TC 1 Z9 1 U1 0 U2 6 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 925 EP 930 DI 10.2134/agronj13.0423 PG 6 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300016 ER PT J AU Hunt, ER Daughtry, CST AF Hunt, E. Raymond, Jr. Daughtry, Craig S. T. TI Chlorophyll Meter Calibrations for Chlorophyll Content Using Measured and Simulated Leaf Transmittances SO AGRONOMY JOURNAL LA English DT Article ID OPTICAL-PROPERTIES MODEL; EXTRACTABLE CHLOROPHYLL; NITROGEN CONCENTRATION; NONDESTRUCTIVE METHOD; SPAD-502 READINGS; PHOTOSYNTHETIC PIGMENTS; LEAVES; CORN; REFLECTANCE; GREENNESS AB Konica-Minolta SPAD-502 leaf chlorophyll meters provide a relative value of leaf chlorophyll content, and from the literature, there are considerable variations among the calibration equations between total chlorophyll contents (mu g chlorophyll a + b cm(-2)) and SPAD-502 values. Our objective was to determine the leaf properties that contributed to the variations in calibration. We determined the internal calibration coefficient of five SPAD-502 meters so that leaf transmittances in the red (650 nm) and near-infrared (940 nm) could be used to calculate SPAD-502 values. A leaf optics model, PROSPECT, was used to simulate transmittances and the chlorophyll-SPAD-502 relationship for different leaf optical properties. Spectral and leaf data from maize (Zea mays L.) showed that PROSPECT predicted leaf transmittances within 2%. Maize leaf data used in the PROSPECT model predicted the relationship between chlorophyll content and the SPAD-502 value, although a polynomial regression was a better fit to the data. There was a physical interaction between chlorophyll content and optical leaf structure affecting leaf transmittances, which is not in the equation for calculating SPAD-502 values. Changing the PROSPECT leaf structure parameter resulted in different chlorophyll-SPAD-502 meter relationships, which were similar to the measured range of variation from calibration equations found in the literature. If the red and near-infrared transmittances are saved for each chlorophyll meter reading, then leaf radiative transfer models such as PROSPECT may be inverted to determine the actual leaf chlorophyll content. C1 [Hunt, E. Raymond, Jr.; Daughtry, Craig S. T.] ARS, USDA, Beltsville Agr Res Ctr, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Hunt, ER (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Hydrol & Remote Sensing Lab, Bldg 007 Room 104 BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM raymond.hunt@ars.usda.gov NR 70 TC 2 Z9 3 U1 2 U2 31 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 931 EP 939 DI 10.2134/agronj13.0322 PG 9 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300017 ER PT J AU Allen, BL Vigil, MF Jabro, JD AF Allen, B. L. Vigil, M. F. Jabro, J. D. TI Camelina Growing Degree Hour and Base Temperature Requirements SO AGRONOMY JOURNAL LA English DT Article ID NORTHERN GREAT-PLAINS; CANOLA; DATE; ROTATIONS; QUALITY; CANADA; SATIVA; YIELD AB Oilseed crops show potential as biofuel feedstocks that can diversify spring wheat (Triticum aestivum L.) rotations in the northern Great Plains. Camelina (Camelina sativa L.) is a relatively new oilseed crop, with limited emergence information available. A 70-d incubator study investigated the impact of temperature (0, 2, 4, and 16 degrees C), seeding depth (3 and 6 mm), and cultivar (Blaine Creek, Calena, Celine, Ligena, and Suneson) on camelina emergence. After 68 d, camelina emergence at 0 degrees C approached 100%. Base temperature averaged -0.70 degrees C for the five cultivars tested and was 19% lower for the 6-than the 3-mm seeding depth, although emergence was 11% sooner for the 3-than the 6-mm seeding depth. About 1150 growing degree hours were required for 50% emergence, which corresponds to 29 March for camelina planted on 10 March, the earliest date when the average daily temperature exceeds the base temperature according to long-term weather records for Sidney, MT. These results suggest that camelina emerges at temperatures below the freezing point of water and that early planting in spring would probably be limited by field access due to wet soil rather than the base temperature requirement. Although camelina emerges at temperatures below freezing under laboratory conditions, further investigation is warranted to confirm emergence under field conditions and determine the frost tolerance of camelina subsequent to germination. C1 [Allen, B. L.; Jabro, J. D.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Vigil, M. F.] ARS, USDA, Cent Great Plains Res Stn, Akron, CO 80720 USA. RP Allen, BL (reprint author), ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. EM brett.allen@ars.usda.gov NR 25 TC 7 Z9 7 U1 3 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 940 EP 944 DI 10.2134/agronj13.0469 PG 5 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300018 ER PT J AU Grabber, JH Jokela, WE Lauer, JG AF Grabber, John H. Jokela, William E. Lauer, Joseph G. TI Soil Nitrogen and Forage Yields of Corn Grown with Clover or Grass Companion Crops and Manure SO AGRONOMY JOURNAL LA English DT Article ID COVER CROP; KURA CLOVER; LIVING MULCH; ALFALFA-GRASS; NITRATE; SYSTEM; WATER; FERTILIZATION; ESTABLISHMENT; MANAGEMENT AB Few studies have compared the agronomic performance of cover crop and living mulch systems for no-till silage corn (Zea mays L.). In a 4-yr Wisconsin study, we evaluated soil N levels and forage yields from manured rotations of corn grown with kura clover (Trifolium ambiguum M. Bieb.) living mulch or interseeded red clover (T. pratense L.) followed by a year of clover production and from manured continuous corn grown with interseeded Italian ryegrass (Lolium multiflorum Lam.), fall-seeded winter rye (Secale cereale L.), or no companion. Companion crops influenced spring and fall nitrate concentrations near the soil surface but had little effect on total residual fall nitrate to a 1.2-m depth. Residual nitrate was not related to N balance (inputs minus outputs), but excessive N inputs into corn-clover systems accumulated as organic soil N. Averaged across both phases of the rotation, corn-clover systems provided 0 to 23% less dry matter yield, but 26 to 60% more crude protein yield than continuous corn systems, with corn-red clover often producing the highest silage corn and clover yields. Kura clover provided superior ground cover and nitrate uptake, but it often excessively competed with corn and had low forage yields. Applying fall manure to ryegrass and spring manure to rye maximized silage yields of continuous corn, but manure application time had no other effect on forage yields or on soil N. Overall, no system excelled in all characteristics, thus selection of companion crop and manure management systems for silage corn will depend on feed production and environmental goals. C1 [Grabber, John H.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Jokela, William E.] ARS, USDA, Inst Environm Integrated Dairy Management, Marshfield, WI 54449 USA. [Lauer, Joseph G.] Univ Wisconsin, Dep Agron, Madison, WI 53706 USA. RP Grabber, JH (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM John.Grabber@ars.usda.gov NR 44 TC 1 Z9 1 U1 9 U2 37 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 952 EP 961 DI 10.2134/agronj13.0466 PG 10 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300020 ER PT J AU Schomberg, HH Fisher, DS Reeves, DW Endale, DM Raper, RL Jayaratne, KSU Gamble, GR Jenkins, MB AF Schomberg, H. H. Fisher, D. S. Reeves, D. W. Endale, D. M. Raper, R. L. Jayaratne, K. S. U. Gamble, G. R. Jenkins, M. B. TI Grazing Winter Rye Cover Crop in a Cotton No-Till System: Yield and Economics SO AGRONOMY JOURNAL LA English DT Article ID DOMAIN REFLECTOMETRY MEASUREMENTS; SORGHUM-FALLOW ROTATION; SOUTHERN PIEDMONT USA; TACQ COMPUTER-PROGRAM; CONSERVATION TILLAGE; PHYSICAL-PROPERTIES; SOIL COMPACTION; CATTLE; PRODUCTIVITY; RESPONSES AB Winter annual cover crop use is limited in conservation management systems in the United States. Grazing cover crops could encourage cover crop adoption if returns offset establishment costs without reducing crop yields. A 4-yr field experiment was conducted near Watkinsville, GA, in which a rye (Secale cereale L.) cover crop was grazed by cattle or roller-crimped before planting cotton (Gossypium hirsutum L.). Cattle consumed about 2.4 Mg ha(-1) of forage annually but amounts were variable due to weather conditions. Rye residue remaining at cotton planting averaged 6.7 Mg ha(-1) for non-grazed compared with 1.7 Mg ha(-1) for grazed treatments. Cotton yields tended to be better in the non-grazed treatment but were significantly different only in 2009 when yields were reduced in the grazed treatment due to soil compaction. Four-year average lint yield was 120 kg ha(-1) greater for the non-grazed treatment. Cotton fiber quality parameters were generally better in the non-grazed treatment but not enough to change crop price. Differences between grazed and non-grazed returns ranged from $-26 to $355 and averaged $81 ha(-1) when based on market year prices. The difference in average return increased to $110 ha(-1) when based on 2012 market year prices. Although negative effects of soil compaction were observed the final year, returns from grazing have the potential to offset establishment costs of a rye cover crop and increase profits for cotton producers in the Southern Piedmont of the United States. C1 [Schomberg, H. H.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Fisher, D. S.] Syngenta Crop Protect, Res Triangle Pk, NC 27703 USA. [Reeves, D. W.] USDA ARS, J Phil Campbell Sr Nat Resource Conservat Ctr, Watkinsville, GA 30677 USA. [Endale, D. M.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. [Raper, R. L.] Oklahoma Agr Exptl Stn, Field Serv Unit, Stillwater, OK 74078 USA. [Raper, R. L.] Oklahoma Agr Exptl Stn, Res Serv Unit, Stillwater, OK 74078 USA. [Jayaratne, K. S. U.] N Carolina State Univ, Dep Agr & Extens Educ, Raleigh, NC 27695 USA. [Gamble, G. R.] USDA ARS, Qual & Safety Assessment Res Unit, Athens, GA 30605 USA. [Jenkins, M. B.] USDA ARS, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. RP Schomberg, HH (reprint author), USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. EM Harry.Schomberg@ars.usda.gov FU Cotton Incorporated; Georgia Commodity Commission for Cotton; USDA ARS FX This research was supported in part by a grant from Cotton Incorporated and the Georgia Commodity Commission for Cotton. Additional support came from USDA ARS base funding. Many individuals contributed to the growing of crops and collection of data and their contributions are greatly appreciated. Robin Woodroof, Stephen Norris, Tony Dillard, Jeff Scarbrough, Eric Elsner, Dwight Seaman, Ryne Branner, Ronald Phillips, Robert Sheats, Clara Parker, Mike Thornton, Eric Schwab, and Shaheen Humayoun provided expert assistance. Ralecia Hamm, Michael Underwood, and James Roper were valuable student helpers on the project. NR 43 TC 3 Z9 3 U1 5 U2 34 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2014 VL 106 IS 3 BP 1041 EP 1050 DI 10.2134/agronj13.0434 PG 10 WC Agronomy SC Agriculture GA AH3SS UT WOS:000336046300029 ER PT J AU Seidler, RG Gese, EM Conner, MM AF Seidler, Renee G. Gese, Eric M. Conner, Mary M. TI Using sterilization to change predation rates of wild coyotes: A test case involving pronghorn fawns SO APPLIED ANIMAL BEHAVIOUR SCIENCE LA English DT Article DE Antilocapra americana; Canis latrans; Coyote; Fawn survival; Predation; Pronghorn antelope; Sterilization ID HOME-RANGE; SURGICAL STERILIZATION; POPULATION-DYNAMICS; TERRITORY FIDELITY; DOMESTIC SHEEP; SURVIVAL RATES; MORTALITY; COMMUNITY; ALBERTA; WOLVES AB Surgical sterilization of coyotes (Canis latrans) reduced their predation rate on domestic sheep. We investigated whether sterilizing coyotes would similarly change coyote predation rates on pronghorn antelope (Antilocapra americana) neonates. From May 2006 to March 2008, we radio-collared 71 pronghorn fawns to determine survival rates in southeast Colorado, USA. During the first year of the study, all coyotes were reproductively intact. During the second year, we surgically sterilized 15 coyotes from 10 packs in the southern half of the study area, while nine coyotes from seven packs in the northern half were given sham sterilizations (i.e., remained reproductively intact). In addition, we estimated the availability of alternative prey and coyote density on both areas to evaluate predator prey factors that could interact with the sterilization treatment. Using the known fate model in Program Mark, we constructed models with and without a treatment effect, plus year, area, individual covariates, alternative prey indices, and predator density to estimate pronghorn fawn survival rates. Results from model averaged parameter estimates and cumulative summer survival indicated coyote sterilization increased survival rates of pronghorn fawns by reducing predation rates of fawns. While fawn survival was higher overall in the north area, after treatment was applied, cumulative pronghorn fawn survival during the summer of 2007 in the south area was 242% higher for pronghorn fawns captured in sterile coyote territories (0.44; 79-day interval survival rate) compared to fawns captured in intact coyote territories (0.18). There was also a significant local area effect, but no relationship between fawn survival and individual fawn covariates of sex, birth weight, birth date, or age. No relationship was detected between fawn survival and lagomorph abundance index, rodent abundance index, or coyote density. Surgical sterilization of coyotes was useful in reducing predation rates on pronghorn fawns. Published by Elsevier B.V. C1 [Seidler, Renee G.; Conner, Mary M.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Gese, Eric M.] Utah State Univ, Wildlife Serv, USDA, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. RP Gese, EM (reprint author), Utah State Univ, Wildlife Serv, USDA, Natl Wildlife Res Ctr,Dept Wildland Resources, Logan, UT 84322 USA. EM eric.gese@usu.edu RI Gese, Eric/B-4578-2011 FU U.S. Department of Agriculture; Wildlife Services; National Wildlife Research Center; Logan Field FX Financial and logistical support provided by the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field. Station at Utah State University, Logan, Utah. Additional support provided by the U.S. Army, Pinon Canyon Maneuver Site, Directorate of Environmental Compliance and Management, Fort Carson, Colorado. We thank Leading Edge Aviation for aerial helicopter captures of coyotes, K. Quigley for conducting the surgeries, M. L. Klavetter, B. F. Smart, and T. Warren for logistical assistance, J. Martinez, J. Miller, M. Oxley, E. Miersma, D. Green, K. Sivy, M. Hatfield, D. Mallett, J. Burghardt, M. L Klavetter, B. F. Smart, M. Greenblatt, S. Gifford, L. Cross, C. Simms, and S. Hollis for field assistance, and J.A. MacMahon for review of the manuscript. NR 75 TC 1 Z9 1 U1 2 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1591 EI 1872-9045 J9 APPL ANIM BEHAV SCI JI Appl. Anim. Behav. Sci. PD MAY PY 2014 VL 154 BP 83 EP 92 DI 10.1016/j.applanim.2014.02.006 PG 10 WC Agriculture, Dairy & Animal Science; Behavioral Sciences; Veterinary Sciences SC Agriculture; Behavioral Sciences; Veterinary Sciences GA AH4OW UT WOS:000336108900011 ER PT J AU Gardner, NM Sachs, AJ Riley, RT Maddox, JR Gelineau-Van Waes, J AF Gardner, N. M. Sachs, A. J. Riley, R. T. Maddox, J. R. Gelineau-Van Waes, J. TI Fumonsin B-1-Induced NTDs: Is Nuclear Sa1P to Blame? SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY LA English DT Meeting Abstract C1 [Gardner, N. M.; Sachs, A. J.; Maddox, J. R.; Gelineau-Van Waes, J.] Creighton Univ, Sch Med, Dept Pharmacol, Omaha, NE 68178 USA. [Riley, R. T.] ARS, Toxicol & Mycotoxin Res Unit, USDA, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1542-0752 EI 1542-0760 J9 BIRTH DEFECTS RES A JI Birth Defects Res. Part A-Clin. Mol. Teratol. PD MAY PY 2014 VL 100 IS 5 SI SI BP 385 EP 385 PG 1 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA AH7AG UT WOS:000336283800048 ER PT J AU Moser, BR AF Moser, Bryan R. TI Preparation and Evaluation of Multifunctional Branched Diesters As Fuel Property Enhancers for Biodiesel and Petroleum Diesel Fuels SO ENERGY & FUELS LA English DT Article ID LOW-TEMPERATURE PROPERTIES; COLD FLOW PROPERTIES; METHYL-ESTERS; FATTY ESTERS; ALKYL ESTERS; ADDITIVES; OIL; BLENDS; IMPACT; PERFORMANCE AB A family of eight branched diesters that were similar in molecular weight to typical fatty acid methyl esters encountered in biodiesel was prepared in high yield by condensation of alcohols and acids. Condensation following a diacid/alcohol route as opposed to the diol/acid method was more facile, as higher yields were obtained in shorter periods of time. The synthetic diesters possessed advantageously low melting points (<-80 degrees C) along with favorable oxidative stabilities (>24 h), densities, flash points, kinematic viscosities (KVs), specific gravities (SGs), and surface tensions. Diesters possessing the most desirable combinations of properties were blended with biodiesel and ultralow sulfur diesel (ULSD) in an effort to ameliorate technical deficiencies of these fuels. Results were compared to relevant biodiesel and petrodiesel fuel standards. Diesters lowered cloud, pour, and cold filter plugging points of biodiesel by up to 5.7 degrees C at 10% (vol) diester. Furthermore, diesters improved lubricity and cold flow properties of ULSD while avoiding deleterious effects on KV, SG, and energy content. Linear responses were noted with regard to the influence of diester concentration on fuel properties in blends with biodiesel and ULSD. In summary, these oxygenated, branched compounds were simple to prepare and were effective at improving the cold flow properties of biodiesel as well as the lubricity of ULSD at low blend levels. C1 USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, Peoria, IL 61604 USA. RP Moser, BR (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov NR 38 TC 3 Z9 3 U1 2 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD MAY PY 2014 VL 28 IS 5 BP 3262 EP 3270 DI 10.1021/ef500482f PG 9 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA AH5VN UT WOS:000336199300045 ER PT J AU Bakota, EL Winkler-Moser, JK Liu, SX AF Bakota, Erica L. Winkler-Moser, Jill K. Liu, Sean X. TI Evaluation of a rice bran oil-derived spread as a functional ingredient SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Edible oil; Oxidative stability; Rice bran oil; Sensory; Spreads ID OXIDATIVE STABILITY; GAMMA-ORYZANOL; VEGETABLE-OILS; FRYING OIL; ROOM ODOR; VITAMIN-E; FATS; QUALITY; FOOD; ANTIOXIDANTS AB As consumers continue to become more interested in the health properties of the food ingredients they purchase, the market potential for new functional ingredients, such as structured lipids and spreadable products, continues to grow. Recently we reported a solvent fractionation procedure for the production of a spreadable product derived from rice bran oil. This material is enriched in phytosterols and rice bran wax relative to crude rice bran oil and has rheological properties that differ vastly from the constituent oil. Here we evaluate the suitability of such a spread for use as a functional ingredient. Two potential avenues are explored: the use of the material as an antioxidant source in frying oils, and the use of this material as a fat replacer in baked goods. As an additive, the material was shown to impart oxidative stability to the oil. This spread was also successfully incorporated into two baked goods with consistently high acceptability ratings for both baked goods tested. Practical applications: We have created a novel semisolid material from crude rice bran oil that can serve as a functional ingredient in a variety of capacities. In this work we incorporate the material into edible oils and baked goods to evaluate its suitability for continued development as an ingredient. In a frying oil, this material may extend the life of the oil by protecting the oil from oxidation during heating. We also show through sensory studies that this material can be successfully used in a bread or granola as a substitute for a portion of the butter or margarine. The high stability and positive sensory reception of this ingredient suggest that this spread might be suitable for further development into a functional food that is allergen-free, trans-fat free, and high in antioxidants. C1 [Bakota, Erica L.; Winkler-Moser, Jill K.; Liu, Sean X.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Bakota, EL (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM erica.bakota@ars.usda.gov NR 35 TC 2 Z9 2 U1 2 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 EI 1438-9312 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD MAY PY 2014 VL 116 IS 5 BP 521 EP 531 DI 10.1002/ejlt.201300259 PG 11 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA AG9PH UT WOS:000335751100003 ER PT J AU Winkler-Moser, JK Logan, A Bakota, EL AF Winkler-Moser, Jill K. Logan, Amy Bakota, Erica L. TI Antioxidant activities and interactions of a- and g-tocopherols within canola and soybean oil emulsions SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Canola oil; Emulsions; Hexanal; Hydroperoxide; Oxidation; Soybean oil; Tocopherols ID OLEIC SUNFLOWER TRIACYLGLYCEROLS; FATTY-ACID-COMPOSITION; GAMMA-TOCOPHEROLS; OXIDATIVE STABILITY; DELTA-TOCOPHEROLS; ALPHA-TOCOPHEROLS; VEGETABLE-OILS; RAPESEED OIL; VITAMIN-E; THERMAL POLYMERIZATION AB The effect of differing concentrations and ratios of - and -TOH on oxidative stability over time was determined by measuring the development of hydroperoxides and volatile secondary oxidation products (hexanal) within a series of oil-in-water (o/w) emulsion systems produced from both canola oil (CO) and soybean oil (SBO). Overall rates for hydroperoxide and hexanal formation were lower in CO than in SBO. In CO emulsions, -TOH was a stronger antioxidant than -TOH, and their interaction in mixtures was additive. In SBO emulsions, -TOH was a better antioxidant at lower concentrations, while -TOH was better at higher concentrations. The interaction between - and -TOH was synergistic at low concentrations, but either additive or antagonistic at higher concentrations, depending on the concentration and ratio of the two TOHs. In both CO and SBO emulsions, -TOH became less effective at higher concentrations, while -TOH activity increased with increasing concentrations. Practical applications: Tocopherols are widely used natural antioxidants in human and pet foods, cosmetics, and supplements. This work provides information about the different antioxidant activities of - and -TOH and their interactions in o/w emulsions made with two of the most widely produced and utilized vegetable oils. This information can be applied to determine the best levels and ratios of tocopherols to protect emulsions depending on the type of oil that is used. C1 [Winkler-Moser, Jill K.; Bakota, Erica L.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Logan, Amy] CSIRO Anim Food & Hlth Sci, Werribee, Vic, Australia. RP Winkler-Moser, JK (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM jill.moser@ars.usda.gov NR 38 TC 2 Z9 2 U1 5 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 EI 1438-9312 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD MAY PY 2014 VL 116 IS 5 BP 606 EP 617 DI 10.1002/ejlt.201300401 PG 12 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA AG9PH UT WOS:000335751100011 ER PT J AU Ngo, HL AF Ngo, Helen L. TI Improved zeolite regeneration processes for preparing saturated branched-chain fatty acids SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Ferrierite zeolite; Isostearic acid; Lubricants; Saturated branched-chain fatty acids ID SKELETAL ISOMERIZATION; ISOMERS AB Ferrierite zeolite solid is an excellent catalyst for the skeletal isomerization of unsaturated linear-chain fatty acids (i.e., oleic acid) to unsaturated branched-chain fatty acids (i.e., iso-oleic acid) follow by hydrogenation to give saturated branched-chain fatty acids (i.e., isostearic acid). In order for the isomerization process to be cost effective, the spent zeolite catalyst must be capable of regeneration for subsequent uses. We report a much improved zeolite regeneration protocol. The Ferrierite zeolite is efficiently regenerated by heating at 115 degrees C for 20h after each use and treatment with an acid solution after every 5th or 6th use. This approach allows the catalyst to be successfully used at least 20 times without significant decrease in conversion and selectivity. The unused and regenerated catalysts have been thoroughly characterized by various analytical techniques. The improved catalyst regeneration protocol should enable cost effective, large scale production of isostearic acid via zeolite-catalyzed skeletal isomerization. C1 ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Ngo, HL (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM helen.ngo@ars.usda.gov NR 24 TC 2 Z9 2 U1 2 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 EI 1438-9312 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD MAY PY 2014 VL 116 IS 5 BP 645 EP 652 DI 10.1002/ejlt.201300315 PG 8 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA AG9PH UT WOS:000335751100015 ER PT J AU Uhlich, GA Chen, CY Cottrell, BJ Nguyen, LH AF Uhlich, Gaylen A. Chen, Chin-Yi Cottrell, Bryan J. Nguyen, Ly-Huong TI Growth media and temperature effects on biofilm formation by serotype O157: H7 and non-O157 Shiga toxin-producing Escherichia coli SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE STEC; serotype O157:H7; biofilm; temperature regulation; Congo red ID IN-GROUND BEEF; SALMONELLA-TYPHIMURIUM; CURLI EXPRESSION; CSGD PROMOTER; CELLULOSE; FIMBRIAE; ENTERITIDIS; RESISTANCE; ORGANELLES; REGULATOR AB Biofilm formation in most Escherichia coli strains is dependent on curli fimbriae and cellulose, and the production of both varies widely among pathogenic strains. Curli and cellulose production by colonies growing on agar are often identified by their affinity for Congo red dye (CR). However, media composition and incubation temperature can affect dye affinity and impose limitations on red phenotype detection by this method. In this study, we compared different Shiga toxin-producing E.coli for CR affinity and biofilm formation under different media/temperature conditions. We found strain and serotype differences in CR affinities and biofilm formation, as well as temperature and media requirements for maximum CR binding. We also constructed strains with deletions of curli and/or cellulose genes to determine their contributions to the phenotypes and identified two O45 strains with a medium-dependent induction of cellulose. C1 [Uhlich, Gaylen A.; Chen, Chin-Yi; Cottrell, Bryan J.; Nguyen, Ly-Huong] USDA ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Uhlich, GA (reprint author), USDA ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM gaylen.uhlich@ars.usda.gov NR 33 TC 12 Z9 12 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD MAY PY 2014 VL 354 IS 2 BP 133 EP 141 DI 10.1111/1574-6968.12439 PG 9 WC Microbiology SC Microbiology GA AH2YJ UT WOS:000335987800008 PM 24702283 ER PT J AU Wang, XQ Li, C Thongda, W Luo, YP Beck, B Peatman, E AF Wang, Xingqiang Li, Chao Thongda, Wilawan Luo, Yupeng Beck, Benjamin Peatman, Eric TI Characterization and mucosal responses of interleukin 17 family ligand and receptor genes in channel catfish Ictalurus punctatus SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Interleukin 17; Interleukin 17 receptor; Mucosal immunity; Catfish ID FLAVOBACTERIUM-COLUMNARE; EDWARDSIELLA-ICTALURI; IL-17 FAMILY; HOST-DEFENSE; INFLAMMATORY MARKERS; BACTERIAL-INFECTION; SIGNAL-TRANSDUCTION; INTESTINAL BARRIER; CYTOKINE FAMILY; TH17 CYTOKINES AB Interleukin (IL) 17 family cytokines are important mediators of mucosa] immune responses, tightly regulated by signals from the complex milieu of pathogenic and commensal microbes, epithelial cells and innate and adaptive leukocytes found at tissue barriers. In mammals, IL17 ligand expression has been linked not only to protective immunity but also excessive tissue inflammation and damage in the gut and lungs. To better understand the scope and action of the IL17 family in channel catfish Ictalurus punctatus, we identified and characterized seven IL17 ligands and four IL17 receptor (IL17R) homologues from transcriptomic and genomic databases. To gain insight into the mucosal actions of the IL17A/Fs-associated pathway in inflammatory processes, the expression profiles of three IL17A/Fs and their putative receptors IL17RA and IL17RC in mucosal tissues of catfish following experimental challenge with Edwardsiella ictaluri and Flavobacterium columnare were investigated. Bacterial challenge induced higher expression of the catfish IL17A/Fs as early at 4 h post-infection, particularly in gill tissue. In contrast, in the catfish intestine, where IL17 function is best understood in mouse models, IL17A/F expression showed minimal early responses to E. ictaluri infection. Instead, a significant up-regulation of IL17 ligands and receptors was observed in the intestine at 7 d, highlighting species and tissue-specific regulation of the IL17 family. (c) 2014 Elsevier Ltd. All rights reserved. C1 [Wang, Xingqiang; Li, Chao; Thongda, Wilawan; Luo, Yupeng; Peatman, Eric] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. [Wang, Xingqiang] Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China. [Beck, Benjamin] USDA, Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Peatman, E (reprint author), Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. EM peatmer@auburn.edu FU Alabama Agriculture Experiment Station Hatch Award [ALA016-1-09035]; Opened Fund of Jiangsu Key Laboratory of Marine Biotechnology [2012HS004]; Natural Science Foundation of Huaihai Institute of Technology [Z2011010]; Special Funds from the Central Finance to Support the Development of Local Universities [CXTD18] FX This project were supported by Alabama Agriculture Experiment Station Hatch Award to E. Peatman under Project ALA016-1-09035, Opened Fund of Jiangsu Key Laboratory of Marine Biotechnology 2012HS004, Natural Science Foundation of Huaihai Institute of Technology Z2011010 and Special Funds from the Central Finance to Support the Development of Local Universities CXTD18. NR 59 TC 13 Z9 15 U1 3 U2 10 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD MAY PY 2014 VL 38 IS 1 BP 47 EP 55 DI 10.1016/j.fsi.2014.02.020 PG 9 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA AH7ZK UT WOS:000336353700007 PM 24602926 ER PT J AU Kamo, K Dilip, L Bauchan, G Rajasekaran, K Cary, J Jaynes, J AF Kamo, K. Dilip, L. Bauchan, G. Rajasekaran, K. Cary, J. Jaynes, J. TI Gladiolus Plants Transformed with D4E1, a Synthetic Antimicrobial Peptide, for Fusarium oxysporum Resistance SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kamo, K.; Dilip, L.] USDA, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Bauchan, G.] Tuskegee Univ, Electron & Confocal Microscopy Unit, Tuskegee, AL 36088 USA. [Rajasekaran, K.; Cary, J.] Tuskegee Univ, USDA, Food & Feed Safety Res Unit, Tuskegee, AL 36088 USA. [Jaynes, J.] Tuskegee Univ, Tuskegee, AL 36088 USA. EM kathryn.kamo@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S57 EP S57 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800141 ER PT J AU Kaya, E Souza, F Rodrigues, L Alves, A Ozudogru, E Maroon-Lango, C Ellis, D AF Kaya, E. Souza, F. Rodrigues, L. Alves, A. Ozudogru, E. Maroon-Lango, C. Ellis, D. TI Development and Comparison of Two Different PVS2-based Procedures for Cryopreservation of Sugarcane (Saccharum spp.) Germplasm. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kaya, E.] Gebze Inst Technol, TR-41400 Kocaeli, Turkey. [Souza, F.] Embrapa Mandioca & Fruticultura Empresa Brasileir, Cruz Das Almas, BA, Brazil. [Rodrigues, L.] Univ Fed Lavras, Lavras, MG, Brazil. [Alves, A.] ARS, USDA, Embrapa Labex USA, Ft Collins, CO USA. [Ozudogru, E.] CNR IVALSA, Ist Valorizzaz Legno & Specie Arboree, Florence, Italy. [Maroon-Lango, C.] USDA APHIS, Plant Germplasm Quarantine Program, Beltsvile, MD USA. [Ellis, D.] Int Potato Ctr, Lima, Peru. EM kayaer19@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S35 EP S35 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800089 ER PT J AU Kochian, L Liu, JP Pineros, M Magalhaes, J AF Kochian, Leon Liu, Jiping Pineros, Miguel Magalhaes, Jurandir TI Plant Adaptation to Mineral Nutrient Stresses in the Soil SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kochian, Leon; Liu, Jiping; Pineros, Miguel] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. [Magalhaes, Jurandir] Embrapa Maize & Sorghum, Sete Lagoas, Brazil. EM LVK1@Cornell.edu NR 0 TC 0 Z9 0 U1 2 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S16 EP S17 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800041 ER PT J AU Lin, J Mazarei, M Zhao, N Rudis, M Pantalone, V Arelli, P Chen, F Stewart, CN AF Lin, J. Mazarei, M. Zhao, N. Rudis, M. Pantalone, V. Arelli, P. Chen, F. Stewart, C. N. TI Overexpression of a Soybean Salicylic Acid Methyltransferase Gene Confers Resistance to Soybean Cyst Nematode. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Lin, J.; Mazarei, M.; Rudis, M.; Pantalone, V.; Chen, F.; Stewart, C. N.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Zhao, N.] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA. [Arelli, P.] ARS, USDA, MSA, Jackson, TN USA. EM jlin11@utk.edu NR 0 TC 0 Z9 0 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S34 EP S35 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800087 ER PT J AU Pence, V Finke, L Niedz, R AF Pence, V. Finke, L. Niedz, R. TI Reducing Hyperhydricity in Shoot Cultures of Cycladenia humilis var. jonesii, an Endangered Dryland Species SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pence, V.; Finke, L.] Cincinnati Zoo & Bot Garden, Cincinnati, OH 45220 USA. [Niedz, R.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM valerie.pence@cincinnatizoo.org NR 0 TC 1 Z9 1 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S62 EP S62 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800157 ER PT J AU Pijut, PM AF Pijut, Paula M. TI Conservation and Development of Fraxinus spp. for Resistance to the Emerald Ash Borer SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Pijut, Paula M.] Purdue Univ, USDA, US Forest Serv, Northern Res Stn,Hardwood Tree Improvement & Rege, W Lafayette, IN 47907 USA. EM ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 1 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S24 EP S24 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800064 ER PT J AU Poothong, S Reed, B AF Poothong, S. Reed, B. TI A Systematic Approach for Optimizing in Vitro mineral Nutrition in Diverse Red Raspberry Germplasm SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Poothong, S.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, B.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM sukalya_p@hotmail.com NR 0 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S17 EP S17 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800042 ER PT J AU Raines, D Dardick, C Callahan, AM Srinivasan, C DeJong, TM Harper, J Castro, S Demuth, M AF Raines, Doug Dardick, Chris Callahan, Ann M. Srinivasan, Chinnathambi DeJong, Ted M. Harper, Jay Castro, Sarah Demuth, Mark TI 'FasTrack' - A Revolutionary Approach to Long-generation Cycle Tree Fruit Breeding SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Raines, Doug; Dardick, Chris; Callahan, Ann M.; Srinivasan, Chinnathambi; Demuth, Mark] USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [DeJong, Ted M.; Castro, Sarah] Univ Calif Davis, Davis, CA 95616 USA. [Harper, Jay] Penn State Univ, University Pk, PA 16802 USA. EM rscorza@afrs.ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S15 EP S16 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800038 ER PT J AU Rupp, J Cruz, L Fellers, J Trick, H AF Rupp, J. Cruz, L. Fellers, J. Trick, H. TI RNAi Mediated Silencing of Endogenous Wheat Genes eIF4E2 and eIF4G Induces Resistance to Potyviruses Wheat Streak Mosaic Virus and Triticum Mosaic Virus SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, J.; Cruz, L.; Trick, H.] Kansas State Univ, Manhattan, KS 66506 USA. [Fellers, J.] ARS, USDA, HWWGRU, Manhattan, KS USA. EM jrupp@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD MAY PY 2014 VL 50 SU 1 BP S31 EP S31 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA AH8KN UT WOS:000336386800078 ER PT J AU Singh, M Byars, JA Kenar, JA AF Singh, Mukti Byars, Jeffrey A. Kenar, James A. TI Amylose-Potassium Oleate Inclusion Complex in Plain Set-Style Yogurt SO JOURNAL OF FOOD SCIENCE LA English DT Article DE amylose-fatty acid complex; rheology; storage; syneresis; texture; yogurt ID TEMPERATURE-INDUCED CHANGES; SODIUM PALMITATE COMPLEXES; CONCENTRATED STARCH GELS; SURFACE-ACTIVE AGENTS; FOOD MODEL SYSTEMS; WAXY WHEAT-STARCH; STIRRED YOGURT; FAT REPLACERS; RHEOLOGICAL PROPERTIES; FLAVOR COMPLEXATION AB Health and wellness aspirations of U.S. consumers continue to drive the demand for lower fat from inherently beneficial foods such as yogurt.Removing fat from yogurt negatively affects the gel strength, texture, syneresis, and storage of yogurt. Amylose-potassium oleate inclusion complexes (AIC) were used to replace skim milk solids to improve the quality of nonfat yogurt. The effect of AIC on fermentation of yogurt mix and strength of yogurt gel was studied and compared to full-fat samples. Texture, storage modulus, and syneresis of yogurt were observed over 4 weeks of storage at 4 degrees C. Yogurt mixes having the skim milk solids partially replaced by AIC fermented at a similar rate as yogurt samples with no milk solids replaced and full-fat milk. Initial viscosity was higher for yogurt mixes with AIC. The presence of 3% AIC strengthened the yogurt gel as indicated by texture and rheology measurements. Yogurt samples with 3% AIC maintained the gel strength during storage and resulted in low syneresis after storage for 4 wk. C1 [Singh, Mukti; Byars, Jeffrey A.; Kenar, James A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Singh, M (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM mukti.singh@ars.usda.gov NR 42 TC 1 Z9 1 U1 4 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP E822 EP E827 DI 10.1111/1750-3841.12378 PG 6 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600009 PM 24712526 ER PT J AU Bhaduri, S Sheen, S Sommers, CH AF Bhaduri, Saumya Sheen, Shiowshuh Sommers, Christopher H. TI Radiation Resistance and Loss of Crystal Violet Binding Activity in Yersinia enterocolitica Suspended in Raw Ground Pork Exposed to Gamma Radiation and Modified Atmosphere SO JOURNAL OF FOOD SCIENCE LA English DT Article DE gamma radiation; modified atmosphere; virulence plasmid; Yersinia enterocolitica ID VIRULENCE PLASMID PYV; ESCHERICHIA-COLI; IONIZING-RADIATION; UNITED-STATES; LACI GENE; IRRADIATION; SURVIVAL; GROWTH; DNA; SUSCEPTIBILITY AB Virulence of many foodborne pathogens is directly linked to genes carried on self-replicating extra-chromosomal elements, which can transfer genetic material, both vertically and horizontally, between bacteria of the same and different species. Pathogenic Yersinia enterocolitica harbors a 70-kb virulence plasmid (pYV) that encodes genes for low calcium response, crystal violet (CV) binding, Congo red uptake, autoagglutination (AA), hydrophobicity (HP), type III secretion channels, host immune suppression factors, and biofilm formation. Ionizing radiation and modified atmosphere packaging (MAP) are used to control foodborne pathogens and meat spoilage. In this study, the effect of gamma radiation and modified atmosphere (air, 100% N-2, 75% N-2: 25% CO2, 50% N-2: 50% CO2, 25% N-2: 75% CO2, 100% CO2) were examined by using the CV binding phenotype, for the presence or absence of pYV in Y. enterocolitica, suspended in raw ground pork. All Y. enterocolitica serovars used (O:3, O:8, and O5,27) were more sensitive to radiation as the CO2 concentration increased above 50%. Crystal violet binding following a radiation dose of 1.0 kGy, which reduced the Y. enterocolitica serovars >5 log, was greatest in the presence of air (ca. 8%), but was not affected by N-2 or CO2 concentration (ca. 5%). Following release from modified atmosphere after irradiation, the loss of CV binding rose from 5% to 8% immediately following irradiation to >30% after outgrowth at 25 degrees C for 24 h. These results, using Y. enterocolitica as a model system, indicate that the risk of foodborne illness could be affected by the loss of virulence factors when postprocess intervention technologies are used. Practical ApplicationProvides gamma radiation D-10 data for inactivation data for Y. enterocolitica irradiated under modified atmosphere and information to risk assessors regarding the difference between pathogen presence versus actual virulence. C1 [Bhaduri, Saumya] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Sheen, Shiowshuh; Sommers, Christopher H.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Bhaduri, S (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM saumya.bhaduri@ars.usda.gov NR 49 TC 1 Z9 1 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP M911 EP M916 DI 10.1111/1750-3841.12413 PG 6 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600019 PM 24761745 ER PT J AU Espitia, PJP Avena-Bustillos, RJ Du, WX Chiou, BS Williams, TG Wood, D McHugh, TH Soares, NFF AF Espitia, Paula J. P. Avena-Bustillos, Roberto J. Du, Wen-Xian Chiou, Bor-Sen Williams, Tina G. Wood, Delilah McHugh, Tara H. Soares, Nilda F. F. TI Physical and Antibacterial Properties of Acai Edible Films Formulated with Thyme Essential Oil and Apple Skin Polyphenols SO JOURNAL OF FOOD SCIENCE LA English DT Article DE acai; antimicrobial activity; edible film; mechanical properties; pectin; polyphenols; thermal stability; thyme essential oil ID COLOR-FLAVOR CORRESPONDENCES; MECHANICAL-PROPERTIES; CELLULOSE WHISKERS; STORAGE STABILITY; DIETARY FIBER; NANOCOMPOSITES; COMPOSITES; CARVACROL; PECTIN; PEEL AB Thyme essential oil (TEO) and apple skin polyphenols (ASP) are natural compounds considered as generally recognized as safe by FDA, with biological effects against bacteria and fungi. This work aimed to evaluate physical and antimicrobial properties of acai edible films formulated with TEO and ASP at 3% and 6% (w/w) individually or combined at 3% (w/w) each. Physical properties studied include mechanical resistance, water vapor permeability (WVP), color, and thermal resistance. Antimicrobial activity against Listeria monocytogenes was determined using the overlay diffusion test. Addition of ASP resulted in improved mechanical properties. TEO at 6% (w/w) resulted in increased elongation. ASP films had significant higher WVP than control film. ASP films were lighter and had more red color than other films. Incorporation of ASP resulted in improved film thermal stability, whereas TEO caused rapid thermal decomposition. Presence of clusters was observed on the surface of films. Addition of ASP resulted in a smother surface, whereas addition of TEO led to the formation of crater-like pits on the film surface. Acai edible film incorporated with 6% (w/w) TEO presented the highest antimicrobial activity. However, both antimicrobials are necessary in the acai films in order to obtain edible films with suitable physical-mechanical properties. The results of the present study showed that TEO and ASP can be used to prepare acai edible films with adequate physical-mechanical properties and antimicrobial activity for food applications by direct contact. Practical ApplicationDeveloped acai edible films presented antimicrobial activity against L. monocytogenes and good physical-mechanical properties, showing the potential use of acai edible films in food preservation. C1 [Espitia, Paula J. P.; Soares, Nilda F. F.] Univ Fed Vicosa, Dept Food Technol, Food Packaging Lab, BR-36570000 Vicosa, MG, Brazil. [Avena-Bustillos, Roberto J.; McHugh, Tara H.] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Chiou, Bor-Sen] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Espitia, PJP (reprint author), Univ Fed Vicosa, Dept Food Technol, Food Packaging Lab, Av PH Rolfs S-N,Campus Univ, BR-36570000 Vicosa, MG, Brazil. EM perez.espitia@gmail.com RI espitia, paula/L-6606-2013 OI espitia, paula/0000-0001-6262-0852 FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES - Brazil) FX The authors thank Mr. Carl Olsen (PFR Unit, USDA/ARS) for providing technical support. Author Espitia gratefully acknowledges financial support from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES - Brazil) through PEC-PG agreement. NR 50 TC 5 Z9 5 U1 0 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP M903 EP M910 DI 10.1111/1750-3841.12432 PG 8 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600018 PM 24749789 ER PT J AU Niemira, BA Boyd, G Sites, J AF Niemira, Brendan A. Boyd, Glenn Sites, Joseph TI Cold Plasma Rapid Decontamination of Food Contact Surfaces Contaminated with Salmonella Biofilms SO JOURNAL OF FOOD SCIENCE LA English DT Article DE biofilm; cold plasma; food safety; Salmonella; sanitizer ID ESCHERICHIA-COLI; FRESH PRODUCE; PRESSURE; O157-H7; INACTIVATION; SENSITIVITY AB Cross-contamination of foods from persistent pathogen reservoirs is a known risk factor in processing environments. Industry requires a rapid, waterless, zero-contact, chemical-free method for removing pathogens from food contact surfaces. Cold plasma was tested for its ability to inactivate Salmonella biofilms. A 3-strain Salmonella culture was grown to form adherent biofilms for 24, 48, or 72 h on a test surface (glass slides). These were placed on a conveyor belt and passed at various line speeds to provide exposure times of 5, 10, or 15 s. The test plate was either 5 or 7.5 cm under a plasma jet emitter operating at 1 atm using filtered air as the feed gas. The frequency of high-voltage electricity was varied from 23 to 48 kHz. At the closer spacing (5 cm), cold plasma reduced Salmonella biofilms by up to 1.57 log CFU/mL (5 s), 1.82 log CFU/mL (10 s), and 2.13 log CFU/mL (15 s). Increasing the distance to 7.5 cm generally reduced the efficacy of the 15 s treatment, but had variable effects on the 5 and 10 s treatments. Variation of the high-voltage electricity had a greater effect on 10 and 15 s treatments, particularly at the 7.5 cm spacing. For each combination of time, distance, and frequency, Salmonella biofilms of 24, 48, and 72 h growth responded consistently with each other. The results show that short treatments with cold plasma yielded up to a 2.13 log reduction of a durable form of Salmonella contamination on a model food contact surface. This technology shows promise as a possible tool for rapid disinfection of materials associated with food processing. Practical ApplicationPathogens such as Salmonella can form chemical-resistant biofilms, making them difficult to remove from food contact surfaces. A 15 s treatment with cold plasma reduced mature Salmonella biofilms by up to 2.13 log CFU/mL (99.3%). This contact-free, waterless method uses no chemical sanitizers. Cold plasma may therefore have a practical application for conveyor belts, equipment, and other food contact surfaces where a rapid, dry antimicrobial process is required. C1 [Niemira, Brendan A.; Boyd, Glenn; Sites, Joseph] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Niemira, BA (reprint author), ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM brendan.Niemira@ars.usda.gov NR 26 TC 6 Z9 6 U1 4 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP M917 EP M922 DI 10.1111/1750-3841.12379 PG 6 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600020 PM 24749764 ER PT J AU Oscar, TP AF Oscar, Thomas P. TI General Regression Neural Network Model for Behavior of Salmonella on Chicken Meat during Cold Storage SO JOURNAL OF FOOD SCIENCE LA English DT Article DE chicken; predictive modeling; Salmonella ID TYPHIMURIUM DT104; PREDICTIVE MODEL; MICROBIAL-GROWTH; MODIFIED ATMOSPHERE; FROZEN STORAGE; GROUND CHICKEN; SURVIVAL; TEMPERATURE; VALIDATION; LISTERIA AB A study was undertaken to investigate and model behavior of Salmonella on chicken meat during cold storage at constant temperatures. Chicken meat (white, dark, or skin) portions (0.75 cm(3)) were inoculated with a single strain of Salmonella Typhimurium DT104 (2.8 log) followed by storage for 0 to 8 d at -8, 0, 8, 12, 14, or 16 degrees C for model development and at -4, 4, 10, or 14 degrees C for model validation. A general regression neural network model was developed with commercial software. Performance of the model was considered acceptable when the proportion of residuals (observed - predicted) in an acceptable prediction zone (pAPZ) from -1 log (fail-safe) to 0.5 logs (fail-dangerous) was 0.7. Growth of Salmonella Typhimurium DT104 on chicken meat was observed at 12, 14, and 16 degrees C and was highest on dark meat, intermediate on skin, and lowest on white meat. At lower temperatures (-8 to 10 degrees C) Salmonella Typhimurium DT104 remained at initial levels throughout 8 d of storage except at 4 degrees C where there was a small (0.4 log) but significant decline. The model had acceptable performance (pAPZ = 0.929) for dependent data (n = 482) and acceptable performance (pAPZ = 0.923) for independent data (n = 235). Results indicated that it is important to include type of meat as an independent variable in the model and that the model provided valid predictions of the behavior of Salmonella Typhimurium DT104 on chicken skin, white, and dark meat during storage for 0 to 8 d at constant temperatures from -8 to 16 degrees C. Practical ApplicationA model for predicting behavior of Salmonella on chicken meat during cold storage was developed and validated. The model will help the chicken industry to better predict and manage this risk to public health. C1 Univ Maryland Eastern Shore, Ctr Food Sci & Technol, Residue Chem & Predict Microbiol Res Unit, USDA,ARS, Princess Anne, MD 21853 USA. RP Oscar, TP (reprint author), Univ Maryland Eastern Shore, Ctr Food Sci & Technol, Residue Chem & Predict Microbiol Res Unit, USDA,ARS, Room 2111, Princess Anne, MD 21853 USA. EM thomas.oscar@ars.usda.gov NR 34 TC 3 Z9 3 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP M978 EP M987 DI 10.1111/1750-3841.12435 PG 10 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600030 PM 24689996 ER PT J AU Sun, XX Narciso, J Wang, Z Ference, C Bai, JH Zhou, KQ AF Sun, Xiuxiu Narciso, Jan Wang, Zhe Ference, Christopher Bai, Jinhe Zhou, Kequan TI Effects of Chitosan-Essential Oil Coatings on Safety and Quality of Fresh Blueberries SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antimicrobial activity; blueberry; chitosan coating; essential oil; postharvest ID ANTIOXIDANT ACTIVITY; IN-VITRO; POSTHARVEST QUALITY; ESCHERICHIA-COLI; SHELF-LIFE; STORAGE; ANTIFUNGAL; ANTIBACTERIAL; TEMPERATURE; CARVACROL AB Chitosan coating plus different essential oils was developed and applied to fresh blueberries in order to find more natural treatments to preserve fresh fruit quality and safety during postharvest storage. Studies were 1st performed in vitro where wild-type Escherichia coli and Penicillium digitatum were grown in suitable media, and then subjected to 6 essential oils. Three compounds, carvacrol (CAR), cinnamaldehyde (CIN), and trans-cinnamaldehyde (ECIN) had high antimicrobial capacity and were selected for an in vivo study for postharvest storage of blueberries. The selected essential oils, 0.5% each, were added into a chitosan solution and coated on fresh blueberries. After storage at 5, 10, and 20 degrees C for various days, fruit firmness and microbial populations were evaluated. The chitosan coating substantially decreased bacteria and yeasts/molds on the fruit, and all 3 essential oils added to the antimicrobial activities. Further dosage experiments showed that the antimicrobial activity remained even when lowering CAR concentration to 0.1% and ECIN to 0.2%. Chitosan, CAR, and ECIN also maintained fruit firmness. Our results suggest that chitosan coatings containing essential oils are effective in extending the shelf life of fresh blueberries. Practical ApplicationBlueberries are high-value fruit with strong antioxidant capacity and other health-promoting benefits. However, microbial food safety is an increasing concern, and decay and softening limits their storability. A combination of 0.1% CAR or 0.2% ECIN with a chitosan coating effectively reduced softening of fresh berries and decay by inhibiting microbial growth. C1 [Sun, Xiuxiu; Zhou, Kequan] Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA. [Narciso, Jan; Wang, Zhe; Ference, Christopher; Bai, Jinhe] ARS, USDA, USHRL, Ft Pierce, FL 34945 USA. RP Zhou, KQ (reprint author), Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA. EM jinhe.bai@ars.usda.gov; kzhou@wayne.edu NR 42 TC 11 Z9 11 U1 5 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2014 VL 79 IS 5 BP M955 EP M960 DI 10.1111/1750-3841.12447 PG 6 WC Food Science & Technology SC Food Science & Technology GA AG5XI UT WOS:000335491600026 PM 24734914 ER PT J AU Aoki, T O'Donnell, K Geiser, DM AF Aoki, Takayuki O'Donnell, Kerry Geiser, David M. TI Systematics of key phytopathogenic Fusarium species: current status and future challenges SO JOURNAL OF GENERAL PLANT PATHOLOGY LA English DT Review DE Biological species; Comparative morphology; Evolution; Molecular phylogeny; Morphological species; Phylogenetic species; Species complex; Species limits; Taxonomy ID SUDDEN-DEATH SYNDROME; VEGETATIVE COMPATIBILITY GROUPS; DNA-SEQUENCE DATABASE; HEAD BLIGHT PATHOGEN; F-SP VASINFECTUM; GIBBERELLA-FUJIKUROI; SP NOV.; GENE GENEALOGIES; FUNGUS FUSARIUM; TRICHOTHECENE BIOSYNTHESIS AB This review is intended to provide plant pathologists and other scientists with a current overview of the most important Fusarium phytopathogens and mycotoxin producers. Knowledge of Fusarium species diversity and their evolutionary relationships has increased dramatically due to the application of multilocus molecular phylogenetics and genealogical concordance phylogenetic species recognition over the past 15 years. Currently Fusarium is estimated to comprise at least 300 genealogically exclusive phylogenetic species; however, fewer than half have been formally described. The most important plant pathogens reside in the following four groups: the F. fujikuroi species complex noted for Bakanae of rice, ear rot of maize, pitch canker of pine and several species that contaminate corn and other cereals with fumonisin mycotoxins; the F. graminearum species complex including the primary agents causing Fusarium head blight of wheat and barley that contaminate grain with trichothecene mycotoxins; the F. oxysporum species complex including vascular wilt agents of over 100 agronomically important crops; and the F. solani species complex, which includes many economically destructive foot and root rot pathogens of diverse hosts. Several other Fusarium phytopathogens reported from Japan and nested within other species complexes are reviewed briefly. With the abandonment of dual nomenclature, a broad consensus within the global community of Fusarium researchers has strongly supported the unitary use of the name Fusarium instead of several teleomorph names linked to it. Plant pathologists and other scientists needing accurate identifications of Fusarium isolates are encouraged to use Fusarium-ID and Fusarium MLST, Internet accessible websites dedicated to the molecular identification of Fusarium species. C1 [Aoki, Takayuki] Natl Inst Agrobiol Sci, Genet Resources Ctr MAFF, Tsukuba, Ibaraki 3058602, Japan. [O'Donnell, Kerry] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 60604 USA. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Aoki, T (reprint author), Natl Inst Agrobiol Sci, Genet Resources Ctr MAFF, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. EM taoki@nias.affrc.go.jp NR 97 TC 18 Z9 19 U1 19 U2 150 PU SPRINGER JAPAN KK PI TOKYO PA CHIYODA FIRST BLDG EAST, 3-8-1 NISHI-KANDA, CHIYODA-KU, TOKYO, 101-0065, JAPAN SN 1345-2630 EI 1610-739X J9 J GEN PLANT PATHOL JI J. Gen. Plant Pathol. PD MAY PY 2014 VL 80 IS 3 BP 189 EP 201 DI 10.1007/s10327-014-0509-3 PG 13 WC Plant Sciences SC Plant Sciences GA AH6XR UT WOS:000336276100001 ER PT J AU Van Ekert, E Heylen, K Rouge, P Powell, CA Shatters, RG Smagghe, G Borovsky, D AF Van Ekert, Evelien Heylen, Kevin Rouge, Pierre Powell, Charles A. Shatters, Robert G., Jr. Smagghe, Guy Borovsky, Dov TI Aedes aegypti juvenile hormone acid methyl transferase, the ultimate enzyme in the biosynthetic pathway of juvenile hormone III, exhibits substrate control SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Corpora allata; Aedes aegypti; Ovary; Juvenile hormone methyltransferase; 3D Modeling; Gene expression ID KEY REGULATORY ENZYME; CORPORA ALLATA; CORPUS ALLATUM; IN-VITRO; DROSOPHILA-MELANOGASTER; FARNESOIC ACID; METHYLTRANSFERASE; PROTEINS; MOSQUITO; RECOGNITION AB We report on the cloning, sequencing, characterization, 3D modeling and docking of Aedes aegypti juvenile hormone acid methyl transferase (AeaJHAMT), the enzyme that converts juvenile hormone acid (JHA) into juvenile hormone (JH). Purified recombinant AeaJHAMT was extensively characterized for enzymatic activity and the Michaelis Menten kinetic parameters K-m, V-max, k(cat) (turn over number) and k(cat)/K-m, (catalytic efficiency) using JHA and its analogues as substrates. AeaJHAMT methylates JHA III 5-fold faster than farnesoic acid (FA). Significant differences in lower methyl transferase (MT) activities towards the cis/trans/trans, cis/trans/cis and the trans/cis/cis isomers of JHA I (1.32, 4.71 and 156-fold, respectively) indicate that substrate chirality is important for proper alignment at the catalytic cavity and for efficient methyl transfer by'S-adenosyl methionine (SAM). Our 3D model shows a potential binding site below the main catalytic cavity for JHA analogues causing conformational change and steric hindrance in the transfer of the methyl group to JHA III. These, in silico, observations were corroborated by, in vitro, studies showing that several JHA analogues are potent inhibitors of AeaJHAMT. In vitro, and in vivo studies using [H-3-methyl]SAM show that the enzyme is present and active throughout the adult life stage of A. aegypti. Tissue specific expressions of the JHAMT gene of A. aegypti (jmtA) transcript during the life cycle of A. aegypti show that AeaJHAMT is a constitutive enzyme and jmtA transcript is expressed in the corpora allata (CA), and the ovary before and after the blood meal. These results indicate that JH III can be synthesized from JHA III by the mosquito ovary, suggesting that ovarian JH III may play an important physiological role in ovarian development and reproduction. Incubating AeaJHAMT with highly pure synthetic substrates indicates that JHA III is the enzyme's preferred substrate, suggesting that AeaJHAMT is the ultimate enzyme in the biosynthetic pathway of JH III. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Van Ekert, Evelien; Powell, Charles A.] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. [Heylen, Kevin] Katholieke Univ Leuven, B-3000 Louvain, Belgium. [Rouge, Pierre] Univ Toulouse 3, UMR UPS IRD 152, F-3106 Toulouse, France. [Shatters, Robert G., Jr.] USDA ARS, Ft Pierce, FL 34945 USA. [Smagghe, Guy] Univ Ghent, B-9000 Ghent, Belgium. [Borovsky, Dov] Borovsky Consulting, Vero Beach, FL 32968 USA. RP Borovsky, D (reprint author), Borovsky Consulting, 135 36th Court, Vero Beach, FL 32968 USA. EM dovborovsky@gmail.com FU DACS; USA-Israel BSF FX We thank Dr. Karl Slama for providing us with the JH analogues and Dr. John Ramos for the help in the statistical analyses. This work was supported by DACS and by USA-Israel BSF Grants to D.B. NR 47 TC 6 Z9 6 U1 0 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2014 VL 64 BP 62 EP 73 DI 10.1016/j.jinsphys.2014.03.001 PG 12 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA AH9NB UT WOS:000336468000009 PM 24657668 ER PT J AU Abdelrahman, H Rinehart, JP Yocum, GD Greenlee, KJ Helm, BR Kemp, WP Schulz, CH Bowsher, JH AF Abdelrahman, H. Rinehart, J. P. Yocum, G. D. Greenlee, K. J. Helm, B. R. Kemp, W. P. Schulz, C. H. Bowsher, J. H. TI Extended hypoxia in the alfalfa leafcutting bee, Megachile rotundata, increases survival but causes sub-lethal effects SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Hypoxia; Megachile rotundata; Diapause; Overwintering; Feeding rate; Fight metabolic rate ID FLUCTUATING THERMAL REGIME; HYMENOPTERA-MEGACHILIDAE; TOBACCO HORNWORM; OXIDATIVE DAMAGE; LIFE EXPECTANCY; MANDUCA-SEXTA; SOLITARY BEE; OXYGEN; BEHAVIOR; HOUSEFLIES AB Many insects are tolerant of hypoxic conditions, but survival may come at a cost to long-term health. The alfalfa leaf-cutting bee, Megachile rotundata, develops in brood cells inside natural cavities, and may be exposed to hypoxic conditions for extended periods of time. Whether M. rotundata is tolerant of hypoxia, and whether exposure results in sub-lethal effects, has never been investigated. Overwintering M. roiundata prepupae were exposed to 10%, 13%, 17%, 21% and 24% O-2 for 11 months. Once adults emerged, five indicators of quality emergence weight, body size, feeding activity, flight performance, and adult longevity, were measured to determine whether adult bees that survived past exposure to hypoxia were competent pollinators. M. rotundata prepupae are tolerant of hypoxic condition and have higher survival rates in hypoxia, than in normoxia. Under hypoxia, adult emergence rates did not decrease over the 11 months of the experiment. In contrast, bees reared in normoxia had decreased emergence rates by 8 months, and were dead by 11 months. M. rotundata prepupae exposed to extended hypoxic conditions had similar emergence weight, head width, and cross-thorax distance compared to bees reared in standard 21% oxygen. Despite no significant morphological differences, hypoxia-exposed bees had lower feeding rates and shorter adult lifespans. Hypoxia may play a role in post-diapause physiology of M. rotundata, with prepupae showing better survival under hypoxic conditions. Extended exposure to hypoxia, while not fatal, causes sub-lethal effects in feeding rates and longevity in the adults, indicating that hypoxia tolerance comes at a cost. (C) 2014 The Authors. Published by Elsevier Ltd. C1 [Abdelrahman, H.; Greenlee, K. J.; Helm, B. R.; Schulz, C. H.; Bowsher, J. H.] N Dakota State Univ, NDSU Dept 2715, Dept Biol Sci, Fargo, ND 58108 USA. [Rinehart, J. P.; Yocum, G. D.; Helm, B. R.; Kemp, W. P.] USDA ARS, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. RP Bowsher, JH (reprint author), N Dakota State Univ, NDSU Dept 2715, Dept Biol Sci, POB 6050, Fargo, ND 58108 USA. EM houda.abdelrahman.1@ndsu.edu; joseph.rinehart@ars.usda.gov; george.yocum@ars.usda.gov; kendra.greenlee@ndsu.edu; bryan.r.helm@ndsu.edu; william.kemp@ars.usda.gov; cody.schulz.1@ndsu.edu; Julia.bowsher@ndsu.edu FU United States Department of Agriculture and North Dakota State; University NSF FORWARD Grant [HRD-0811239]; NSF [IOS 0953297] FX The authors thank Meghan Bennett, Keeley Cook, Carlee O'Dell, Marnie Larson, Lindsey Fransen, and Karen Dickey for their technical assistance. We thank two anonymous reviewers for their comments. This work was supported by the United States Department of Agriculture and North Dakota State. University NSF FORWARD Grant HRD-0811239 (JB) and NSF IOS 0953297 (KJG). NR 33 TC 2 Z9 2 U1 1 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2014 VL 64 BP 81 EP 89 DI 10.1016/j.jinsphys.2014.03.007 PG 9 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA AH9NB UT WOS:000336468000011 PM 24662466 ER PT J AU Wilson, ML Allan, DL Baker, JM AF Wilson, Melissa L. Allan, Deborah L. Baker, John M. TI Aerially seeding cover crops in the northern US Corn Belt: Limitations, future research needs, and alternative practices SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID REDUCE NITRATE LOSS; POSTDISPERSAL PREDATION; MANURE INJECTION; UPPER MIDWEST; SWINE MANURE; WEED SEEDS; RYE; ROTATION; TILLAGE; ESTABLISHMENT C1 [Wilson, Melissa L.] Community Coll Baltimore Cty Baltimore, Baltimore, MD 21215 USA. [Allan, Deborah L.] Univ Minnesota St Paul, Dept Soil Water & Climate, St Paul, MN USA. [Baker, John M.] Univ Minnesota St Paul, ARS, USDA, St Paul, MN USA. RP Wilson, ML (reprint author), Community Coll Baltimore Cty Baltimore, Baltimore, MD 21215 USA. NR 56 TC 1 Z9 1 U1 3 U2 20 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2014 VL 69 IS 3 BP 67A EP 72A DI 10.2489/jswc.69.3.67A PG 6 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AH5US UT WOS:000336197200001 ER PT J AU Printz, JL Toledo, D Boltz, SC AF Printz, Jeffrey L. Toledo, David Boltz, Stanley C. TI Rangeland health assessment: The key to understanding and assessing rangeland soil health in the Northern Great Plains SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article C1 [Printz, Jeffrey L.] Nat Resources Conservat Serv, USDA, Bismarck, ND 58502 USA. [Toledo, David] ARS, USDA, No Great Plains Res Lab, Mandan, ND USA. [Boltz, Stanley C.] Nat Resources Conservat Serv, USDA, Huron, SD USA. RP Printz, JL (reprint author), Nat Resources Conservat Serv, USDA, Bismarck, ND 58502 USA. RI Toledo, David/A-9259-2010 OI Toledo, David/0000-0001-5162-4428 NR 12 TC 2 Z9 2 U1 0 U2 13 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2014 VL 69 IS 3 BP 73A EP 77A DI 10.2489/jswc.69.3.73A PG 5 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AH5US UT WOS:000336197200002 ER PT J AU Bolster, CH Horvath, T Lee, BD Mehilhope, S Higgins, S Delgado, JA AF Bolster, C. H. Horvath, T. Lee, B. D. Mehilhope, S. Higgins, S. Delgado, J. A. TI Development and testing of a new phosphorus index for Kentucky SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE Kentucky; nutrient management; phosphorus index ID WATER-EXTRACTABLE PHOSPHORUS; SURFACE-APPLIED MANURES; TALL FESCUE PASTURE; DISSOLVED PHOSPHORUS; POULTRY LITTER; SOIL-PHOSPHORUS; NITROGEN LOSSES; ASSESSMENT-TOOL; FIELD PLOTS; RUNOFF LOSS AB The phosphorus index (PI) is a field-scale assessment tool developed to identify fields most vulnerable to phosphorus (P) loss. The USDA Natural Resource Conservation Service (NRCS) recently revised its 590 Nutrient Management Standard and Title 190 National Instruction requiring that all NRCS-approved PI tools meet certain criteria. A recent study evaluating the Kentucky PI showed that it did not meet several of the criteria established by NRCS. This paper describes the development and evaluation of a revised PI for Kentucky in response to the revised 590 Standard. Important revisions to the Kentucky PI include (1) use of a component formulation, (2) incorporation of erosion and P application rates, (3) use of continuous variables, and (4) use of empirically based weighting factors. The revised Kentucky PI was evaluated against measured P loss data reported in the literature. Output from the revised PI was well correlated (Spearman's rho = 0.86; p < 0.001) with the measured P loss data. Results also indicated that the revised Kentucky PI correctly assigned the appropriate risk category to the majority of fields with P loss values below or above our predefined cutoff values for low and high risk fields. On the other hand, the revised PI only correctly categorized 43% of the fields deemed to be at moderate risk. To assess whether the revised PI provided improved estimates of P loss risk, output from both the original and revised Kentucky PIs was compared against a P loss data set collected in the southern United States. Both the original (after modifications to soil test P [STP] and runoff ratings) and revised Kentucky PIs were significantly correlated with the measured P loss data, though the revised PI (Spearman's rho = 0.92; p < 0.001) was more strongly correlated than the original Kentucky PI (Spearman's rho = 0.42; p = 0.03). The original and revised Kentucky PIs were also compared by calculating P loss risk for 46 fields in Kentucky using data recently collected for developing comprehensive nutrient management plans. The approach adopted in this study to revise the Kentucky PI should offer guidance to other states currently in the process of revising their PI. C1 [Bolster, C. H.] USDA ARS, Food Anim Environm Syst Res Unit Bowling Green, Bowling Green, KY 42104 USA. [Horvath, T.] Nat Resources Conservat Serv Lexington, USDA, Lexington, KY USA. [Lee, B. D.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40506 USA. [Mehilhope, S.] Univ Kentucky, Dept Biosyst & Agr Engn, Lexington, KY 40506 USA. [Higgins, S.] Univ Kentucky, Coll Agr, Agr Expt Stn, Lexington, KY 40506 USA. [Delgado, J. A.] ARS, USDA, Soil Plant Nutrient Res Unit Ft Collins, Ft Collins, CO 80526 USA. RP Bolster, CH (reprint author), USDA ARS, Food Anim Environm Syst Res Unit Bowling Green, Bowling Green, KY 42104 USA. NR 67 TC 1 Z9 3 U1 0 U2 25 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2014 VL 69 IS 3 BP 183 EP 196 DI 10.2489/jswc.69.3.183 PG 14 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AH5US UT WOS:000336197200006 ER PT J AU Wang, X Amonett, C Williams, JR Wilcox, BP Fox, WE Tu, MC AF Wang, X. Amonett, C. Williams, J. R. Wilcox, B. P. Fox, W. E. Tu, M. -C. TI Rangeland watershed study using the Agricultural Policy/Environmental eXtender SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE Agricultural Policy/Environmental eXtender; conservation practices; sediment; watershed modeling ID SEDIMENT PRODUCTION; APEX MODEL; CONSERVATION PRACTICES; SENSITIVITY-ANALYSIS; MANAGEMENT-PRACTICES; SOUTHERN PLAINS; SOIL-EROSION; TEXAS; VALIDATION; RUNOFF AB Information on grazing land conservation practices and their effectiveness in controlling nonpoint source pollution and watershed health at the watershed/landseape scale is necessary for future planning and resource allocation. The objectives of this study were to test the ability of the Agricultural Policy/Environmental eXtender (APEX) model to simulate hydrologic and soil processes at the watershed scale and utilize the model to evaluate the benefit of rangeland in reducing sediment loading and the benefit of potential conservation practices within a rangeland watershed. The model was calibrated and validated for stream flow and sediment yield for two watersheds within the Cowhouse Creek Watershed in north-central Texas: Cowhouse Creek Watershed upstream of Pidcoke (CCUP) Watershed (1,178 km(2) [290,966 ac]) and Clear Creek (CC) Watershed (57.3 km(2) [14,153 ac]). Nash-Sutcliffe efficiencies for the watersheds ranged from 0.62 to 0.71 and 0.71 to 0.81 for monthly and annual stream flow, and from 0.51 to 0.84 and 0.60 to 0.84 for monthly and annual sediment yields, respectively. The percent errors ranged from -9.3% to 12.7% for stream flow and from -22.6% to 11.5% for sediment yield. In addition to APEX model calibration/validation, various modeling scenarios (e.g., cropland expansion and brush control/reduced grazing rate conservation practices) were derived and analyzed for the CCUP watershed.The cropland expansion scenario analysis (less than 0.1% continuous corn to 39% continuous corn) indicated that the current rangeland has the benefit of reducing sediment loading by 65%. Rangeland conservation practice scenario modeling identified substantial reductions in overland sediment losses for conversion of range brush to range grass on Evant soil areas, with an average reduction of 58.8% from treated areas. However, the sediment loading to the CCUP watershed outlet at Pidcoke was only reduced by 7% due to the low treated area of 6%.The reducing stocking rate scenario was less effective compared with brush removal since the current 10 ha cattle(-1) (24 ac cattle(-1)) was not considered a heavy stocking rate. Reducing the current stocking rate to 15 ha cattle(-1) (37 ac cattle(-1)) reduced sediment loading by 5%. This study shows that the APEX model is able to replicate measured tream flow and sediment yields for rangeland watersheds with satisfactory performance based on well-accepted statistical criteria. C1 [Wang, X.] Texas A&M Univ Syst Temple, Texas AgriLife Res, Blackland Res & Extens Ctr, College Stn, TX 77843 USA. [Amonett, C.] Nat Resource Conservat Serv Temple, USDA, Temple, TX USA. [Williams, J. R.; Fox, W. E.] Blackland Res & Extens Ctr Temple, Temple, TX USA. [Wilcox, B. P.; Tu, M. -C.] Texas A&M Univ, College Stn, TX 77843 USA. RP Wang, X (reprint author), Texas A&M Univ Syst Temple, Texas AgriLife Res, Blackland Res & Extens Ctr, College Stn, TX 77843 USA. FU USDA Natural Resource Conservation Service through the Conservation Effect Assessment Project (CEAP) [2008-51130-19562]; USDA National Institute of Food andAgricultute, Managed Ecosystem [2010-85101-20485] FX This study was supported by the USDA Natural Resource Conservation Service through the Conservation Effect Assessment Project (CEAP) entitled Watershed consavation on grazing lands in central Texas: Cowhouse Creek case study with CEAP Grant No 2008-51130-19562 and USDA National Institute of Food andAgricultute, Managed Ecosystem Grant No. 2010-85101-20485. USDA is an equal opportunity provider and employer NR 66 TC 2 Z9 2 U1 2 U2 16 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2014 VL 69 IS 3 BP 197 EP 212 DI 10.2489/jswc.69.3.197 PG 16 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AH5US UT WOS:000336197200007 ER PT J AU Green, AK Jacques, PF Rogers, G Fox, CS Meigs, JB McKeown, NM AF Green, Angela K. Jacques, Paul F. Rogers, Gail Fox, Caroline S. Meigs, James B. McKeown, Nicola M. TI Sugar-Sweetened Beverages and Prevalence of the Metabolically Abnormal Phenotype in the Framingham Heart Study SO OBESITY LA English DT Article ID FOOD-FREQUENCY QUESTIONNAIRE; INSULIN-RESISTANCE; HEALTHY OBESE; CARDIOVASCULAR-DISEASE; NORMAL-WEIGHT; AGED ADULTS; US ADULTS; RISK; CONSUMPTION; REPRODUCIBILITY AB Objective: The purpose of this study was to examine the relationship between usual sugar-sweetened beverage (SSB) consumption and prevalence of abnormal metabolic health across body mass index (BMI) categories. Methods: The metabolic health of 6,842 non-diabetic adults was classified using cross-sectional data from the Framingham Heart Study Offspring (1998-2001) and Third Generation (2002-2005) cohorts. Adults were classified as normal weight, overweight or obese and, within these categories, metabolic health was defined based on five criteria-hypertension, elevated fasting glucose, elevated triglycerides, low HDL cholesterol, and insulin resistance. Individuals without metabolic abnormalities were considered metabolically healthy. Logistic regression was used to examine the associations between categories of SSB consumption and risk of metabolic health after stratification by BMI. Results: Comparing the highest category of SSB consumers (median of 7 SSB per week) to the lowest category (non-consumers), odds ratios (95% confidence intervals) for metabolically abnormal phenotypes, compared to the metabolically normal, were 1.9 (1.1-3.4) among the obese, 2.0 (1.4-2.9) among the overweight, and 1.9 (1.4-2.6) among the normal weight individuals. Conclusions: In this cross-sectional analysis, it is observed that, irrespective of weight status, consumers of SSB were more likely to display metabolic abnormalities compared to non-consumers in a dosedependent manner. C1 [Green, Angela K.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Jacques, Paul F.; Rogers, Gail; McKeown, Nicola M.] Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Fox, Caroline S.] NHLBI, Framingham Heart Study, Framingham, MA USA. [Fox, Caroline S.] Brigham & Womens Hosp, Div Endocrinol Metab & Diabet, Dept Med, Boston, MA 02115 USA. [Fox, Caroline S.; Meigs, James B.] Harvard Univ, Sch Med, Boston, MA USA. [Meigs, James B.] Massachusetts Gen Hosp, Dept Med, Div Gen Med, Boston, MA 02114 USA. RP McKeown, NM (reprint author), Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU National Heart Lung and Blood Institute [NO1-HC-25195]; USDA Agricultural Research Service [58-1950-0-014] FX This study was supported by grants from the National Heart Lung and Blood Institute (contract NO1-HC-25195) and the USDA Agricultural Research Service (agreement 58-1950-0-014). The funding providers did not play a role in any aspect of this study. NR 36 TC 4 Z9 4 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD MAY PY 2014 VL 22 IS 5 BP E157 EP E163 DI 10.1002/oby.20724 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AH3OR UT WOS:000336034400024 PM 24550031 ER PT J AU Beck, JJ Mahoney, NE Cook, D Higbee, BS Light, DM Gee, WS Baig, N AF Beck, John J. Mahoney, Noreen E. Cook, Daniel Higbee, Bradley S. Light, Douglas M. Gee, Wai S. Baig, Nausheena TI Comparison of the volatile emission profiles of ground almond and pistachio mummies: Part 2-Critical changes in emission profiles as a result of increasing the water activity SO PHYTOCHEMISTRY LETTERS LA English DT Article DE Almond; Host plant volatiles; Mummies; Navel orangeworm; Pistachio; Water activity ID ORANGEWORM LEPIDOPTERA-PYRALIDAE; NAVEL ORANGEWORM; EGG TRAPS; CALIFORNIA; MOTHS; BAIT AB Pistachio and almond mummies have been reported to attract adult navel orangeworm moths in field trapping studies. The volatile profiles of these matrices were recently described. Concurrently, recent investigations have demonstrated that the water activity of almond hulls plays an important role in the production of semiochemicals known to attract the navel orangeworm in almond orchards. In the present study, the water activity of pistachio and almond mummies was increased and the resultant headspace volatiles monitored over the course of a week. The volatile profile of wet pistachio mummies contained 86 volatiles, of which 22 were unique to the wet matrix. The volatile profile of the wet pistachio matrix increased in chemical diversity to include small chain alcohols, benzenoids, and fatty acid breakdown products relative to the dry matrix, which primarily emitted terpenoids. The wet almond mummies emitted a total of 57 volatiles, 37 more than dry almond mummies. Among the volatiles detected in the wet almond mummies were three of the five compounds that are found in the synthetic blend of host plant volatiles known to attract navel orangeworm moths. The volatile bouquets from the wet and dry pistachio mummies, and wet almond mummies were evaluated by electroantennographic (EAG) analysis. Published by Elsevier Ireland Ltd on behalf of Phytochemical Society of Europe. C1 [Beck, John J.; Mahoney, Noreen E.; Light, Douglas M.; Gee, Wai S.; Baig, Nausheena] Agr Res Serv, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Cook, Daniel] Agr Res Serv, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. RP Beck, JJ (reprint author), Agr Res Serv, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM john.beck@ars.usda.gov FU USDA-ARS CRIS [5325-42000-037-00D]; TFCA [5325-42000-037-00D]; RCA [5325-42000-037-13] FX The authors thank Inna Ovchinnikova, Yvonne Ho (USDA-ARS), Johnny Magana, and Ashlee Pedro (Paramount Farming) for their valuable contributions. Research was conducted under USDA-ARS CRIS Project 5325-42000-037-00D, TFCA 5325-42000-037-00D with the California Pistachio Research Board, and RCA 5325-42000-037-13 with the California Department of Food and Agriculture. NR 18 TC 6 Z9 6 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3900 EI 1876-7486 J9 PHYTOCHEM LETT JI Phytochem. Lett. PD MAY PY 2014 VL 8 BP 220 EP 225 DI 10.1016/j.phytol.2014.01.004 PG 6 WC Plant Sciences; Chemistry, Medicinal SC Plant Sciences; Pharmacology & Pharmacy GA AG9ML UT WOS:000335743300045 ER PT J AU Chaudet, MM Naumann, TA Price, NPJ Rose, DR AF Chaudet, Marcia M. Naumann, Todd A. Price, Neil P. J. Rose, David R. TI Crystallographic structure of ChitA, a glycoside hydrolase family 19, plant class IV chitinase from Zea mays SO PROTEIN SCIENCE LA English DT Article DE chitinase; chitin; crystal structure; plant defense; mass spectrometry; enzyme catalysis ID SUBSTRATE-BINDING SUBSITES; 26 KDA ENDOCHITINASE; HORDEUM-VULGARE L; CRYSTAL-STRUCTURE; CATALYTIC DOMAIN; BARLEY-SEEDS; MAIZE SEED; MODE; IDENTIFICATION; MECHANISMS AB Maize ChitA chitinase is composed of a small, hevein-like domain attached to a carboxy-terminal chitinase domain. During fungal ear rot, the hevein-like domain is cleaved by secreted fungal proteases to produce truncated forms of ChitA. Here, we report a structural and biochemical characterization of truncated ChitA (ChitA N), which lacks the hevein-like domain. ChitA N and a mutant form (ChitA N-EQ) were expressed and purified; enzyme assays showed that ChitA N activity was comparable to the full-length enzyme. Mutation of Glu62 to Gln (ChitA N-EQ) abolished chitinase activity without disrupting substrate binding, demonstrating that Glu62 is directly involved in catalysis. A crystal structure of ChitA N-EQ provided strong support for key roles for Glu62, Arg177, and Glu165 in hydrolysis, and for Ser103 and Tyr106 in substrate binding. These findings demonstrate that the hevein-like domain is not needed for enzyme activity. Moreover, comparison of the crystal structure of this plant class IV chitinase with structures from larger class I and II enzymes suggest that class IV chitinases have evolved to accommodate shorter substrates. PDB Code(s): C1 [Chaudet, Marcia M.; Rose, David R.] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada. [Naumann, Todd A.] USDA ARS NCAUR, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. [Price, Neil P. J.] USDA ARS NCAUR, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. RP Rose, DR (reprint author), Univ Waterloo, Dept Biol, 200 Univ Ave West, Waterloo, ON N2L 3G1, Canada. EM drrose@uwaterloo.ca FU Canadian Natural Science and Engineering Research Council FX Grant sponsor: Discovery Grant from the Canadian Natural Science and Engineering Research Council. NR 37 TC 5 Z9 5 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 EI 1469-896X J9 PROTEIN SCI JI Protein Sci. PD MAY PY 2014 VL 23 IS 5 BP 586 EP 593 DI 10.1002/pro.2437 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AF3DU UT WOS:000334592500008 PM 24616181 ER PT J AU Hirsch-Schantz, MC Monaco, TA Call, CA Sheley, RL AF Hirsch-Schantz, Merilynn C. Monaco, Thomas A. Call, Christopher A. Sheley, R. L. TI Large-Scale Downy Brome Treatments Alter Plant-Soil Relationships and Promote Perennial Grasses in Salt Desert Shrub lands SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE cheatgrass; disturbance ecology; ecosystem resilience; restoration ecology; soil resource dynamics; spatial and temporal variation ID GREAT-BASIN; RESOURCE AVAILABILITY; SPECIES RICHNESS; PRESCRIBED FIRE; ARID GRASSLAND; TECTORUM; INVASION; CHEATGRASS; NITROGEN; COMMUNITIES AB Because invasive annual grasses can strongly influence soil resource availability and disturbance regimes to favor their own persistence, there is a great need to understand the interrelationships among invasive plant abundance, resource availability, and desirable species prominence. These interrelationships were studied in two salt desert sites where the local abundance of downy brome (Bromus tectorurn L.) varied spatially and increased more than 12-fold over a 3-yr period. We measured downy brome percentage cover, resource availability, and soil chemical and physical properties within 112 plots per site and found significant negative associations between downy brome abundance and both soil water content (P < 0.05; r=-0.27 to -0.49) and nitrate accumulation (P < 0.05; r=-0.34 to -0.45), which corroborated with the direction and strength of multivariate factor loadings assessed with principal component analysis. We then applied factorial combinations of prescribed burning and preemergence herbicide at management-relevant scales (i.e., 6 to 46 ha) as well as biomass removal to smaller plots (12.25 m(2)) at both sites to determine their impact on downy brome, soil resources, and resident plant species. Burning and herbicide applications, especially when combined, significantly reduced downy brome cover (P=0.069 to 0.015), which in turn increased soil nitrate accumulation and water content in the spring. Furthermore, for one shrubland site that was seeded 6 yr previously, the combination of burning and herbicide treatments significantly increased perennial grass percentage cover in the 2 yr posttreatment (P < 0.05). Results not only demonstrate the strong relationships between downy brome abundance, soil resources, and residence species for impoverished salt desert shrub ecosystems, but also suggest that restoration and management efforts must include tactics that facilitate resource use by the residual plant community or establish a greater abundance of species capable of high resource acquisition in the spring. C1 [Hirsch-Schantz, Merilynn C.; Call, Christopher A.] Utah State Univ, Wildland Resources Dept, Logan, UT 84322 USA. [Monaco, Thomas A.] Agr Res Serv, USDA, Forage & Range Res Labs, Logan, UT 84322 USA. [Sheley, R. L.] Agr Res Serv, USDA, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Hirsch-Schantz, MC (reprint author), Oregon State Univ, Dept Rangeland Ecol & Management, Corvallis, OR 97331 USA. EM hirschm@onid.orst.edu FU USDA, Agricultural Research Service, Area-Wide Project on Ecologically Based Invasive Plant Management EBIPM of Annual Grasses in the Great Basin FX Research was funded by USDA, Agricultural Research Service, Area-Wide Project on Ecologically Based Invasive Plant Management EBIPM of Annual Grasses in the Great Basin. We thank Justin Williams, Beth Fowers, Robert Watson, Casey Snieder, Joe Lamb, Sarahi Felix, and Rui-Xiang Liu for assistance with field and laboratory research. We also thank Bob Blank and Lora Perkins, whose insightful reviews improved this article. NR 56 TC 2 Z9 2 U1 5 U2 28 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 255 EP 265 DI 10.2111/REM-D-13-00101.1 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300004 ER PT J AU Liebig, MA Kronberg, SL Hendrickson, JR Gross, JR AF Liebig, M. A. Kronberg, S. L. Hendrickson, J. R. Gross, J. R. TI Grazing Management, Season, and Drought Contributions to Near-Surface Soil Property Dynamics in Semiarid Rangeland SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE electrical conductivity; greenhouse gas emissions; Northern Plains; soil acidification ID NORTHERN GREAT-PLAINS; ELECTRICAL-CONDUCTIVITY; CHEMICAL-PROPERTIES; CRESTED WHEATGRASS; PASTURE MANAGEMENT; NATIVE RANGELANDS; GRASS PRAIRIE; ARABLE SOIL; CARBON; FIELD AB Grazing management effects on soil property dynamics are poorly understood. A study was conducted to assess effects of grazing management and season on soil property dynamics and greenhouse gas flux within semiarid rangeland. Grazing management treatments evaluated in the study included two permanent pastures differing in stocking rate (moderately and heavily grazed pastures) and a fertilized, heavily grazed crested wheatgrass (Agropyron desertorum [Fisch. ex. Link] Schult.) pasture near Mandan, North Dakota. Over a period of 3 yr, soil properties were measured in the spring, summer, and fall at 0-5 cm and 5-10 cm. Concurrent to soil-based measurements, fluxes of carbon dioxide, methane, and nitrous oxide were measured on 1-wk to 2-wk intervals and related to soil properties via stepwise regression. High stocking rate and fertilizer nitrogen (N) application within the crested wheatgrass pasture contributed to increased soil bulk density and extractable N, and decreased soil pH and microbial biomass compared to permanent pastures. Soil nitrate nitrogen tended to be greatest at peak aboveground biomass, whereas soil ammonium nitrogen was greatest in early spring. Drought conditions during the third year of the study contributed to nearly two-fold increases in extractable N under the crested wheatgrass pasture and the heavily grazed permanent pasture, but not the moderately grazed permanent pasture. Stepwise regression found select soil properties to be modestly related to soil atmosphere greenhouse gas fluxes, with model r(2) ranging from 0.09 to 0.76. Electrical conductivity was included most frequently in stepwise regressions and, accordingly, may serve as a useful screening indicator for greenhouse gas "hot spots" in grazing land. C1 [Liebig, M. A.; Kronberg, S. L.; Hendrickson, J. R.] USDA ARS, Mandan, ND 58554 USA. [Gross, J. R.] USDA ARS, Rifle, CO 81650 USA. RP Liebig, MA (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM mark.liebig@ars.usda.gov FU USDA Agricultural Research Service GRACEnet Project; Long Term Agro-Ecosystem Research Network FX This publication is based on work supported by the USDA Agricultural Research Service GRACEnet Project and Long Term Agro-Ecosystem Research Network. NR 55 TC 5 Z9 5 U1 0 U2 27 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 266 EP 274 DI 10.2111/REM-D-13-00145.1 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300005 ER PT J AU Haubensak, KA D'Antonio, CM Embry, S Blank, R AF Haubensak, Karen A. D'Antonio, Carla M. Embry, Saundra Blank, Robert TI A Comparison of Bromus tectorum Growth and Mycorrhizal Colonization in Salt Desert vs. Sagebrush Habitats SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE AMF; invader; maternal effects; seed source soil nutrients ID HEAT-SHOCK PROTEINS; ECOLOGICAL GENETICS; LOCAL ADAPTATION; PLANT INVASION; STRESS; FUNGI; ENVIRONMENTS; POPULATIONS; CHEATGRASS; TOLERANCE AB Cheatgrass (Bromus tectorum) has recently invaded marginal low-elevation salt desert habitats across the Great Basin. We tested the hypothesis that cheatgrass seed produced in populations from the more stressful salt desert vs. upland sagebrush habitats should grow differently in salt desert soils compared to adjacent upland sagebrush soil, and vice versa. We evaluated growth, incidence of flowering, and arbuscular mycorrhizal fungi (AMF) colonization of plants grown in the soils from which their seeds were collected vs. in the reciprocal soils from the nearest sagebrush or salt desert site in three large basins in northern Nevada. Simultaneously we measured nutrient cations, available nitrogen and phosphorus, percent carbon and nitrogen, texture, and dry-down characteristics in all soils. We found that salt desert soils were generally more nutrient poor and more saline than their upland (sagebrush) counterparts; salt desert soils also generally had a higher percentage of sand compared to their upland counterparts and were consistently drier. The most dramatic plant responses to soil and seed source were 1) lower aboveground biomass of mature plants in most salt desert soils compared to sagebrush soils, or lower biomass in plants grown from salt desert seed; 2) lower root:shoot ratios in plants grown in salt desert soil across two of three basins, irrespective of seed source; 3) a higher percentage of flowering individuals from salt desert seed sources at harvest, irrespective of soil source; 4) depressed AMF colonization of plants in salt desert soils; and 5) strong influence exerted by seed source on AMF, whereby sagebrush-originating plants grown in sagebrush soils had greater AMF colonization compared to salt desert soils but salt desert-originating seedlings had very low AMF colonization rates irrespective of soil source. These results suggest that both population level and soil-based controls are important as this widespread weed moves into marginal habitat. C1 [Haubensak, Karen A.; D'Antonio, Carla M.; Blank, Robert] USDA ARS, Exot & Invas Weeds Res Unit, Reno, NV 89512 USA. [Embry, Saundra] Univ Nevada, Reno, NV 89557 USA. RP Haubensak, KA (reprint author), No Arizona Univ, Dept Biol Sci, Box 5640, Flagstaff, AZ 86011 USA. EM karen.haubensak@nau.edu FU US Dept of Agriculture-Agricultural Research Service FX Research was funded by the US Dept of Agriculture-Agricultural Research Service. NR 45 TC 5 Z9 5 U1 4 U2 28 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 275 EP 284 DI 10.2111/REM-D-12-00024.1 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300006 ER PT J AU Morris, LR Monaco, TA Sheley, RL AF Morris, L. R. Monaco, T. A. Sheley, R. L. TI Impact of Cultivation Legacies on Rehabilitation Seedings and Native Species Re-Establishment in Great Basin Shrub lands SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE crested wheatgrass; exarable fields; land-use legacies; old fields; sagebrush; site history ID WESTERN-AUSTRALIA; SOIL; VEGETATION; RECOVERY; COVER; RESTORATION; GERMINATION; MANAGEMENT; RANGELANDS; ECOLOGY AB Little is known about how cultivation legacies affect the outcome of rehabilitation seedings in the Great Basin, even though both frequently co-occur on the same lands. Similarly, there is little known about how these legacies affect native species reestablishment into these seedings. We examined these legacy effects by comparing areas historically cultivated and seeded to adjacent areas that were seeded but never cultivated, for density of seeded crested wheatgrass (Agropyron cristatum [L.] Gaertn.) and native perennial grasses, vegetation cover, and ground cover. At half of the sites, historically cultivated areas had lower crested wheatgrass density (P < 0.05), and only one site had a higher density of crested wheatgrass (P < 0.05). Likewise, the native shrub Wyoming big sagebrush (Artemisia tridentata Nutt. subsp. wyomingensis Beetle & Young) had lower cover (P < 0.05) in historically cultivated areas at half the sites. Sandberg bluegrass (Poa secunda J. Presl.) density was consistently lower in historically cultivated areas relative to those seeded-only. At sites where black greasewood (Sarcobatus vermiculatus [Hook.] Torr.) and bottlebrush squirreltail (Elymus elymoides [Raf.] Swezey) were encountered, there was either no difference or a higher density and cover within historically cultivated areas (P < 0.05). Likewise, cover of exotic forbs, especially halogeton (Halogeton glomeratus [M. Bieb.] C. A. Mey.), was either not different or higher in historically cultivated areas (P < 0.05). Bare ground was greater in historically cultivated areas at three sites (P < 0.05). These results suggest that cultivation legacies can affect seeding success and re-establishment of native vegetation, and therefore should not be overlooked when selecting research sites or planning land treatments that include seeding and or management to achieve greater native species diversity. C1 [Morris, L. R.] Oregon State Univ, Dept Anim & Rangeland Sci, La Grande, OR 97850 USA. [Monaco, T. A.] Utah State Univ, Agr Res Serv, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. [Sheley, R. L.] Agr Res Serv, USDA, Range & Meadow Forage Management Res, Burns, OR 97720 USA. RP Morris, LR (reprint author), Eastern Oregon Univ, OSU Ag Program, Dept Anim & Rangeland Sci, Oregon State Univ, 205 Badgley Hall, La Grande, OR 97850 USA. EM Lesley.Morris@oregonstate.edu FU USDA Agricultural Research Service Area-wide Ecologically Based Invasive Plant Management project FX This research was funded by the USDA Agricultural Research Service Area-wide Ecologically Based Invasive Plant Management project. NR 51 TC 3 Z9 3 U1 2 U2 24 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 285 EP 291 DI 10.2111/REM-D-12-00147.1 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300007 ER PT J AU Boyd, CS Obradovich, M AF Boyd, Chad S. Obradovich, Matthew TI Is Pile Seeding Wyoming Big Sagebrush (Artemisia tridentata subsp wyomingensis) an Effective Alternative to Broadcast Seeding? SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE competition; restoration; sagebrush restoration; seedling; survival ID PLANT-COMMUNITIES; SAGE-GROUSE; MINED LANDS; GRASS; HETEROGENEITY; ESTABLISHMENT; GERMINATION; COMPETITION; DISPERSAL; ABUNDANCE AB Sagebrush plays an important role in the ecological functions of sagebrush steppe plant communities and is a necessary component of habitat for a variety of wildlife including greater sage-grouse (Centrocercus urophasianus). At lower elevations, increased fire frequency associated with exotic annual grass invasion has heightened the need for effective sagebrush restoration strategies, but existing techniques have been largely ineffective. Our objective was to evaluate "pile seeding" (placing mature seed heads on the ground) of Wyoming big sagebrush (Artemisia tridentata subsp. wyomingensis) as an alternative to broadcast seeding. We used a randomized block design (n=.5) replicated in 2 yr at two contrasting ecological sites in southeastern Oregon. Treatments applied to 100 X 1.5 m plots included 1) pile seeding (four mature seed heads center dot pile(-1) x 10 piles center dot plot(-1)), 2) broadcast seeding (0.5 kg pure live seed [PLS] center dot ha(-1)), and 3) natural recovery (i.e., nonseeded). Planting occurred in fall 2008 and 2009, and plots were monitored for seedling establishment for three or two growing seasons postplanting. Seedling density was estimated at the plot scale within a SO-cm radius of each seed head pile ("island scale"). In the year following planting, sagebrush seedling density at the plot scale was up to 60-fold higher (P <= 0.05) in pile-seeded plots compared to natural recovery and broadcast plots. Seedling mortality was high (up to 98% reduction in density) for pile-seeded plots between the first and second growing seasons postplanting and differences between broadcast and pile-seeded plots dissipated by 2-3 yr postplanting. Although pile-seeding had higher initial density than broadcast seeding, neither technique had sufficient multiyear survival to suggest restoration efficacy at the plot scale. Seedling density at the island scale suggests that pile-seeding may be useful for establishing sagebrush islands, depending on year conditions. Research is needed to determine strategies capable of increasing long-term sagebrush seedling survival. C1 [Boyd, Chad S.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Obradovich, Matthew] US Dept Interior, Bur Land Management, Hines, OR 97738 USA. RP Boyd, CS (reprint author), USDA ARS, EOARC, 67826-A Hwy 205, Burns, OR 97720 USA. EM chad.boyd@oregonstate.edu NR 45 TC 4 Z9 4 U1 1 U2 13 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 292 EP 297 DI 10.2111/REM-D-13-00107.1 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300008 ER PT J AU Dufek, NA Vermeire, LT Waterman, RC Ganguli, AC AF Dufek, N. A. Vermeire, L. T. Waterman, R. C. Ganguli, A. C. TI Fire and Nitrogen Addition Increase Forage Quality of Aristida purpurea SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE digestibility; gas production; in vitro fermentation; prescribed burning; purple threeawn; silica ID GAS-PRODUCTION PROFILES; NORTHERN GREAT-PLAINS; MIXED-GRASS PRAIRIE; IN-VITRO; TALLGRASS PRAIRIE; CHEMICAL-COMPOSITION; SEMIARID SAVANNA; BIG BLUESTEM; DIET QUALITY; SOUTH-AFRICA AB Purple threeawn (Aristida purpurea Nutt.) is a native perennial bunchgrass with limited forage value that dominates sites with disturbed soils and persists with repeated severe grazing. Fire and nitrogen addition have been used to reduce threeawn and can increase grazing utilization of threeawn by livestock. We evaluated effects of fire, spring urea addition, and phenological stage on purple threeawn forage quality 1 yr postfire on two similar sites in southeastern Montana during the 2011 (site 1) and 2012 (site 2) growing seasons. Fire (no fire, summer fire, fall fire) and rate of nitrogen addition (0, 46, 80 kg N center dot ha(-1)) were arranged in a completely randomized, fully factorial design. Samples were collected at five phenological stages throughout each growing season. Forage quality was assessed using nutrient analyses of crude protein (CP), net energy (NEm), and total digestible nutrients; antiquality analyses of neutral detergent fiber (NDF), acid detergent fiber, and silica; in vitro fermentation for organic matter disappearance (IVOMD) and NDF disappearance; and gas production (asymptotic [maximum] gas production, fractional rate of gas production, lag time, and average fermentation rate). In vegetative stages, summer and fall fire increased CP from 6.2% to 12.1% and 13.0%, respectively, and NDF decreased from 72.1% to 69.4% and 68.2%, respectively. Summer and fall fire reduce silica content from 7.0% to 4.1% and 4.3%, respectively. Purple threeawn IVOMD increased by 14.0% and 13.0% following summer and fall fire, respectively, compared to nonburned plots. Nitrogen addition increased CP from 7.5% to 8.0% and 8.4%, respectively, with 46% and 80 kg N center dot ha(-1), respectively. In vitro fermentation and gas production variables did not change due to nitrogen addition. Fire generally improved purple threeawn forage quality to a greater extent than did nitrogen addition. Results indicate fire can potentially improve the suitability of purple threeawn as a forage species. C1 [Dufek, N. A.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82001 USA. [Vermeire, L. T.; Waterman, R. C.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Ganguli, A. C.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Vermeire, LT (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM lance.vermeire@ars.usda.gov RI Ganguli, Amy/J-3342-2014 OI Ganguli, Amy/0000-0003-3960-1404 FU Bureau of Land Management; Agricultural Experiment Station (AES) FX The authors would like to thank Whisper Kelly, Susan Reil, Brook Shipp, Dustin Strong, Morgan Russell, and the entire staff at the USDA-ARS Fort Keogh Livestock and Range Research Laboratory for their tireless help in data collection, animal care, equipment maintenance, and treatment implementation. Drs. Gary Clambey and Kevin Sedivec provided valuable feedback on earlier versions of this manuscript. Major funding and logistical support was provided by the Bureau of Land Management. Additional funding was provided by the Agricultural Experiment Station (AES) Dean's Fellowship. We would also like to thank the Jens and Tibbets families as well as the Bureau of Land Management for their cooperation in establishing and maintaining our research plots in Terry, Montana. NR 61 TC 2 Z9 2 U1 3 U2 8 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 298 EP 306 DI 10.2111/REM-D-13-00057.1 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300009 ER PT J AU Hulet, A Roundy, BA Petersen, SL Jensen, RR Bunting, SC AF Hulet, April Roundy, Bruce A. Petersen, Steven L. Jensen, Ryan R. Bunting, Stephen C. TI Cover Estimations Using Object-Based Image Analysis Rule Sets Developed Across Multiple Scales in Pinyon-Juniper Woodlands SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE eCognition Developer; high spatial resolution imagery; object-based image analysis; rule sets; SageSTEP ID GROUND-BASED METHODS; GREAT-BASIN; RANGELAND; AERIAL AB Numerous studies have been conducted that evaluate the utility of remote sensing for monitoring and assessing vegetation and ground cover to support land management decisions and complement ground measurements. However, few comparisons have been made that evaluate the utility of object-based image analysis (OBIA) to accurately classify a landscape where rule sets (models) have been developed at various scales. In this study, OBIA rule sets used to estimate land cover from high spatial resolution imagery (0.06-m pixel) on Pinus L. (pinyon) and Juniperus L. (juniper) woodlands were developed using eCognition Developer at four scales with varying grains-1) individual plot, 2) individual sites, 3) regions (western juniper vs. Utah juniper sites), and 4) pinyon-juniper woodland network (all plots) that were within the same study extent. Color-infrared imagery was acquired over five sites in Oregon, California, Nevada, and Utah with a Vexcel UltraCamX digital camera in June 2009. Ground cover measurements were also collected at study sites in 2009 on 80 0.1-ha plots. Correlations between OBIA and ground measurements were relatively high for individual plot and site rule sets (ranging from r=0.52 to r=0.98). Correlations for regional and network rule sets were lower (ranging from r=0.24 to r=0.63), which was expected due to radiance differences between the images as well as vegetation differences found at each site. All site and plot OBIA average cover percentage estimates for live trees, shrubs, perennial herbaceous vegetation, litter, and bare ground were within 5% of the ground measurements, and all region and network OBIA average cover percentage estimates were within 10%. The trade-off for decreased accuracy over a larger area (region and network rule sets) may be useful to prioritize management strategies but will unlikely capture subtle shifts in understory plant communities that site and plot rule sets often capture. C1 [Hulet, April] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Roundy, Bruce A.; Petersen, Steven L.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Jensen, Ryan R.] Brigham Young Univ, Dept Geog, Provo, UT 84602 USA. [Bunting, Stephen C.] Univ Idaho, Dept Rangeland Ecol & Management, Moscow, ID 83844 USA. RP Hulet, A (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. EM april.hulet@oregonstate.edu RI Ma, Lei/I-4597-2014 FU Joint Fire Sciences Sagebrush Steppe Treatment Evaluation Project; Bureau of Land Management; Brigham Young University FX Research was funded in part by the Joint Fire Sciences Sagebrush Steppe Treatment Evaluation Project, the Bureau of Land Management, and Brigham Young University. NR 35 TC 2 Z9 2 U1 2 U2 18 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 318 EP 327 DI 10.2111/REM-D-12-00154.1 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300011 ER PT J AU Beck, JL Booth, DT Kennedy, CL AF Beck, Jeffrey L. Booth, D. Terrance Kennedy, Carmen L. TI Assessing Greater Sage-Grouse Breeding Habitat With Aerial and Ground Imagery SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE big sagebrush; Centrocercus urophasianus; rangeland monitoring and assessment; sampling costs; very large scale aerial imagery ID SAGEBRUSH HABITATS; RANGELAND; COVER AB Anthropogenic disturbances, wildfires, and weedy-plant invasions have destroyed and fragmented many sagebrush (Artemisia L. spp.) habitats. Sagebrush-dependent species like greater sage-grouse (Centrocercus urophasianus) are vulnerable to these changes, making habitat monitoring essential to effective management. Conventional ground inventory methods are time consuming (expensive) and have lower data collection potentials than remote sensing. Our study evaluated the feasibility of ground (0.3-mm ground surface distance [GSD]) and aerial imagery (primarily, 1-mm GSD) to assess ground cover for big sagebrush (Artemisia tridentata Nutt.) and other vegetation functional groups important in sage-grouse breeding habitat (lekking, nesting, and brood rearing). We surveyed similar to 526 km(2) of the upper Powder River watershed in Natrona County, Wyoming, USA, a region dominated by Wyoming big sagebrush (Artemisia tridentata Nutt. ssp. wyomingensis Beetle & Young) communities interspersed with narrow riparian corridors. Our study area was used year-round by sage-grouse and included 16 leks. In June 2010, we acquired aerial images (1-mm resolution) for 3228 systematic sampling locations; additional images were acquired as rapid-succession bursts where aerial transects crossed riparian areas and for 39 riparian and 39 upland ground locations (0.3-mm resolution) within 3.2-km of leks. We used SamplePoint software to quantify cover for plant taxa and functional groups using all ground images and a systematic sampling of aerial images. Canopy cover of sage-grouse food forbs-as averaged across aerial and ground imagery around all leks-was 1.8% and 7.8% in riparian and 0.5% and 4.0% in upland areas, respectively. Big sagebrush cover was 8.7% from upland aerial images and 9.4% from upland ground images. Aerial and ground imagery provided similar values for bare ground and shrubs in riparian and upland areas, whereas ground imagery provided finer-scale herbaceous-cover data that complemented the aerial imagery. These and other image-derived archival data provide a practical basis for landscape-scale management and are a cost-effective means for monitoring extensive sagebrush habitats. C1 [Beck, Jeffrey L.] Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. [Booth, D. Terrance; Kennedy, Carmen L.] USDA ARS, High Plains Grasslands Res Stn, Cheyenne, WY 82009 USA. RP Beck, JL (reprint author), Univ Wyoming, Dept Ecosyst Sci & Management, Dept 3354,1000 E Univ Ave, Laramie, WY 82071 USA. EM jlbeck@uwyo.edu FU USDI-BLM Casper Field Office FX We thank L. Griffith for collecting ground images, J. Wambeke and C. Weber for image processing, and T. Shelton and K. Smith for data summarization. We received technical support and funding through C. Fifield and B. Burgess of the USDI-BLM Casper Field Office. S. Cox of USDA-ARS (currently USDI-BLM, Cheyenne) and J. Binfet from the Wyoming Game and Fish Department provided logistical support. C. Aldridge and C. Buchanan provided helpful comments to an earlier draft. J. Nance, proprietor of Cloud Street Aerial Services (http://www.cloudstreet.net/About_Us.html), Fort Collins, Colorado, conducted aerial flights. NR 23 TC 2 Z9 2 U1 2 U2 36 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2014 VL 67 IS 3 BP 328 EP 332 DI 10.2111/REM-D-12-00141.1 PG 5 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH7XI UT WOS:000336348300012 ER PT J AU Tome, D Jahoor, F Kurpad, A Michaelsen, KF Pencharz, P Slater, C Weisell, R AF Tome, D. Jahoor, F. Kurpad, A. Michaelsen, K. F. Pencharz, P. Slater, C. Weisell, R. TI Current issues in determining dietary protein quality and metabolic utilization SO EUROPEAN JOURNAL OF CLINICAL NUTRITION LA English DT Editorial Material C1 [Tome, D.] AgroParisTech, UMR0914, Paris, France. [Jahoor, F.] USDA ARS, CNRC, Houston, TX USA. [Kurpad, A.] St Johns Natl Acad Hlth Sci, St Johns Med Coll, Bangalore, Karnataka, India. [Michaelsen, K. F.] Univ Copenhagen, Fac Sci, Dept Nutr Exercise & Sports, Frederiksberg C, Denmark. [Pencharz, P.] Univ Toronto, Hosp Sick Children, Toronto, ON M5G 1X8, Canada. [Slater, C.] IAEA, Div Human Hlth, Nutr & Hlth Related Environm Studies Sect, A-1400 Vienna, Austria. RP Tome, D (reprint author), AgroParisTech, UMR0914, Paris, France. EM tome@agroparistech.fr NR 5 TC 3 Z9 3 U1 0 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0954-3007 EI 1476-5640 J9 EUR J CLIN NUTR JI Eur. J. Clin. Nutr. PD MAY PY 2014 VL 68 IS 5 BP 537 EP 538 DI 10.1038/ejcn.2014.55 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AH2FC UT WOS:000335935900001 PM 24801502 ER PT J AU Bilbao-Sainz, C Chiou, BS Valenzuela-Medina, D Du, WX Gregorski, KS Williams, TG Wood, DF Glenn, GM Orts, WJ AF Bilbao-Sainz, Cristina Chiou, Bor-Sen Valenzuela-Medina, Diana Du, Wen-Xian Gregorski, Kay S. Williams, Tina G. Wood, Delilah F. Glenn, Greg M. Orts, William J. TI Solution blow spun poly(lactic acid)/hydroxypropyl methylcellulose nanofibers with antimicrobial properties SO EUROPEAN POLYMER JOURNAL LA English DT Article DE Solution blow spinning; Nanofibers; Poly(lactic acid); Hydroxypropyl methylcellulose; Antimicrobial ID CONTROLLED-RELEASE; FIBERS; ACID); CELLULOSE; DELIVERY; ANTIBIOTICS AB Poly(lactic acid) (PLA) nanofibers containing hydroxypropyl methylcellulose (HPMC) and tetracycline hydrochloride (THC) were solution blow spun from two different solvents, chloroform/acetone (CA, 80:20 v/v) and 2,2,2-triflouroethanol (TFE). The diameter distribution, chemical, thermal, thermal stability, water sorption, and antimicrobial properties were examined for the fibers. Fibers spun from CA generally had larger fiber diameters and wider fiber diameter distributions than those spun from TFE. Fourier transform infrared (FTIR) spectroscopy results indicated successful incorporation of HPMC and THC into the fibers. Also, phase separation occurred between PLA and HPMC in the fibers. Fibers containing higher HPMC concentrations showed greater water sorption values, due to HPMC being more hydrophilic than PLA. In addition, fibers containing HPMC had larger inhibitory zones against Escherichia Coli and Listeria monocytogenes than those without HPMC. This was due to THC having better miscibility with HPMC than PLA and HPMC being able to swell and release more THC when in contact with water. Fibers spun from TFE and CA had comparable inhibitory zones, indicating the solvents did not affect antimicrobial properties. All fibers remained effective against bacteria even after six days. Published by Elsevier Ltd. C1 [Bilbao-Sainz, Cristina; Chiou, Bor-Sen; Valenzuela-Medina, Diana; Du, Wen-Xian; Gregorski, Kay S.; Williams, Tina G.; Wood, Delilah F.; Glenn, Greg M.; Orts, William J.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Chiou, BS (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM bor-sen.chiou@ars.usda.gov NR 26 TC 11 Z9 12 U1 2 U2 48 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0014-3057 EI 1873-1945 J9 EUR POLYM J JI Eur. Polym. J. PD MAY PY 2014 VL 54 BP 1 EP 10 DI 10.1016/j.eurpolymj.2014.02.004 PG 10 WC Polymer Science SC Polymer Science GA AH4PR UT WOS:000336111000001 ER PT J AU Wanjura, JD Barnes, EM Keley, MS Holt, GA Pelletier, MG AF Wanjura, John D. Barnes, Edward M. Keley, Mark S. Holt, Gregory A. Pelletier, Mathew G. TI Quantification and characterization of cotton crop biomass residue SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biomass; Cotton; Energy; Fertility; Feed; Harvest ID BY-PRODUCTS; DRY-MATTER; GROWTH; YIELD AB Cotton crop residual biomass remaining in the field after mechanical seed cotton harvest is not typically harvested and utilized off-site thereby generating additional revenue for producers. Recently, interest has increased in utilizing biomass materials as feedstock for the production of fuel and composite materials. This work was undertaken to investigate the quantity and characteristics of residual biomass remaining in the field for cotton produced in the Southern High Plains of the US. Three growing locations were studied and varied with regard to irrigation use and application system, and harvest method. In order to differentiate the amount of biomass available before and after seed cotton harvest as well as the physical location of the material, biomass yield was measured for five collection groups: (1) pre-harvest stalk, (2) pre-harvest ground, (3) post-harvest stalk, (4) post-harvest ground, and (5) bur trial (for the two stripper harvested locations only). Within each collection group, the biomass was separated into four components: seed cotton, stick, bur, and other vegetative material (OVM). Total biomass produced at the Lubbock (sub-surface drip irrigated, machine stripped), Floyd (non-irrigated, machine stripped), and Dawson (center pivot irrigated, machine picked) locations were 8643, 3227, and 6456 kg DM/ha, respectively. After mechanical seed cotton harvest, 3537 (41%), 1578 (49%), and 4210 (65%) kg DM/ha remained in the fields at the Lubbock, Floyd, and Dawson locations, respectively. Analytical analysis of the individual biomass components indicated that stick and bur have properties more favorable to application as feedstock for biofuel or bio-based composite production whereas OVM is better used as a soil amendment or animal feed ingredient. Cotton producers must consider costs associated with harvest, storage, and transportation of biomass materials but also costs associated with replacement fertilizer and reduced soil tilth prior to making the decision to remove residual cotton crop biomass for off-site use. Published by Elsevier B.V. C1 [Wanjura, John D.; Holt, Gregory A.; Pelletier, Mathew G.] USDA ARS, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. [Barnes, Edward M.] Cotton Inc, Cary, NC 27513 USA. [Keley, Mark S.] Texas A&M AgriLife Extens, Lubbock, TX 79403 USA. RP Wanjura, JD (reprint author), USDA ARS CPPRU, 1604 E FM 1294, Lubbock, TX 79403 USA. EM john.Wanjura@ars.usda.gov FU Cotton Incorporated FX The authors gratefully acknowledge the equipment support of John Deere, financial support of Cotton Incorporated, and the technician staff at the USDA - ARS Cotton Production and Processing Research Unit in Lubbock, TX in this work. NR 34 TC 5 Z9 5 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2014 VL 56 BP 94 EP 104 DI 10.1016/j.indcrop.2014.02.019 PG 11 WC Agricultural Engineering; Agronomy SC Agriculture GA AH3IZ UT WOS:000336018200012 ER PT J AU Sparks, JT Bohbot, JD Dickens, JC AF Sparks, Jackson T. Bohbot, Jonathan D. Dickens, Joseph C. TI The genetics of chemoreception in the labella and tarsi of Aedes aegypti SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Aedes aegypti; Olfactory receptor; lonotropic receptor; Mosquito; Taste; Odorant-binding protein ID IONOTROPIC GLUTAMATE RECEPTORS; NEURON MEMBRANE-PROTEINS; ODORANT-BINDING-PROTEINS; YELLOW-FEVER MOSQUITO; CANDIDATE TASTE RECEPTORS; MALE COURTSHIP BEHAVIOR; ANOPHELES-GAMBIAE; PHEROMONE-BINDING; DIFFERENTIAL EXPRESSION; DROSOPHILA-MELANOGASTER AB The yellow-fever mosquito Aedes aegypti is a major vector of human diseases, such as dengue, yellow fever, chikungunya and West Nile viruses. Chemoreceptor organs on the labella and tarsi are involved in human host evaluation and thus serve as potential foci for the disruption of blood feeding behavior. In addition to host detection, these contact chemoreceptors mediate feeding, oviposition and conspecific recognition; however, the molecular landscape of chemoreception in these tissues remains mostly uncharacterized. Here we report the expression profile of all putative chemoreception genes in the labella and tarsi of both sexes of adult Ae. aegypti and discuss their possible roles in the physiology and behavior of this important disease vector. Published by Elsevier Ltd. C1 [Sparks, Jackson T.; Bohbot, Jonathan D.; Dickens, Joseph C.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Dickens, JC (reprint author), ARS, USDA, BARC, IIBBL, Bldg 007,Rm 030,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov FU Deployed War Fighter Protection (DWFP) Research Program - Department of Defense through the Armed Forces Pest Management Board (AFPMB) FX The authors thank Dr. Shawn E. Levy and Nripesh Prasad of the Genomics Services Lab at the Hudson-Alpha Institute for Biotechnology for RNA sequencing and data analyses. We are grateful to Drs. Richard Vogt, Department of Biological Sciences, University of South Carolina and Daniel Strickman for critical review of the manuscript. This work was supported in part by a grant to J.C.D. from the Deployed War Fighter Protection (DWFP) Research Program funded by the Department of Defense through the Armed Forces Pest Management Board (AFPMB). NR 96 TC 13 Z9 13 U1 7 U2 35 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAY PY 2014 VL 48 BP 8 EP 16 DI 10.1016/j.ibmb.2014.02.004 PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA AH1HN UT WOS:000335871300002 PM 24582661 ER PT J AU Bohbot, JD Sparks, JT Dickens, JC AF Bohbot, Jonathan D. Sparks, Jackson T. Dickens, Joseph C. TI The maxillary palp of Aedes aegypti, a model of multisensory integration SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Aedes aegypti; Olfaction; Mosquito; Maxillary palp; Thermosensation; Mechanosensation ID IONOTROPIC GLUTAMATE RECEPTORS; MOSQUITO ANOPHELES-GAMBIAE; ODORANT-BINDING-PROTEINS; GUSTATORY-RECEPTOR; DROSOPHILA-MELANOGASTER; CARBON-DIOXIDE; TRP CHANNELS; GENE FAMILY; CULEX-QUINQUEFASCIATUS; CHEMOSENSORY RECEPTORS AB Female yellow-fever mosquitoes, Aedes aegypti, are obligate blood-feeders and vectors of the pathogens that cause dengue fever, yellow fever and Chikungunya. This feeding behavior concludes a series of multisensory events guiding the mosquito to its host from a distance. The antennae and maxillary palps play a major role in host detection and other sensory-mediated behaviors. Compared to the antennae, the maxillary palps are a relatively simple organ and thus an attractive model for exploration of the neuromolecular networks underlying chemo-and mechanosensation. In this study, we surveyed the expressed genetic components and examined their potential involvement with these sensory modalities. Using Illumina sequencing, we identified the transcriptome of the maxillary palps of physiologically mature female Ae. aegypti. Genes expressed in the maxillary palps included those involved in sensory reception, signal transduction and neuromodulation. In addition to previously reported chemosensory genes, we identified candidate transcripts potentially involved in mechanosensation and thermosensation. This survey lays the groundwork to explore sensory networks in an insect appendage. The identification of genes involved in thermosensation provides prospective molecular targets for the development of chemicals aimed at disrupting the behavior of this medically important insect. Published by Elsevier Ltd. C1 [Bohbot, Jonathan D.; Sparks, Jackson T.; Dickens, Joseph C.] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Dickens, JC (reprint author), USDA ARS, BARC, PSI, IIBBL, Bldg 007,Rm 030,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov FU Deployed War Fighter Protection (DWFP) Research Program - Department of Defense through the Armed Forces Pest Management Board (AFPMB) FX We wish to thank Dr. Bryan Vinyard for his contribution to the statistical analyses and Dr. Shawn E. Levy and Nripesh Prasad at the Genomic Services Lab at Hudson Alpha Institute for Biotechnology for Illumina sequencing and data analyses. This work was supported in part by a grant to J.C.D. from the Deployed War Fighter Protection (DWFP) Research Program funded by the Department of Defense through the Armed Forces Pest Management Board (AFPMB). NR 125 TC 10 Z9 10 U1 3 U2 35 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 EI 1879-0240 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD MAY PY 2014 VL 48 BP 29 EP 39 DI 10.1016/j.ibmb.2014.02.007 PG 11 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA AH1HN UT WOS:000335871300004 PM 24613607 ER PT J AU Riley, DG Chase, CC Coleman, SW Olson, TA AF Riley, D. G. Chase, C. C., Jr. Coleman, S. W. Olson, T. A. TI Evaluation of the Criollo breed Romosinuano as purebred and crossbred cows with Brahman and Angus in Florida: I. Reproduction and parturition SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE Angus; Brahman; calving rate; crossbreeding; heterosis; Romosinuano ID BOS INDICUS BREEDS; BEEF-CATTLE; CALF MORTALITY; MATERNAL HETEROSIS; PREWEANING TRAITS; INDIVIDUAL TRAITS; SIMMENTAL CATTLE; HEREFORD; PERFORMANCE; LONGEVITY AB The objectives of this work were to compare reproduction and parturition traits of the Criollo breed Romosinuano as straightbred and crossbred cows with Angus and Brahman, to estimate heterosis and direct and maternal genetic breed effects, and to describe calf loss, cow removals from the project, the occurrence of calving difficulty, inadequate calf vigor at birth, and udder problems by cow breed groups. Cows (n = 404) were born from 2002 to 2005. After their first exposure to bulls as young cows, in all subsequent breeding seasons crossbred cows were bred to bulls of the third breed, and straightbred cows were bred to bulls of the other two breeds. Calving records (n = 1,484) from 2005 to 2011 were used to create calving and weaning rate and calving interval (excluding the interval between 2 and 3 yr of age). Final models for these traits included sire breed-dam breed interaction, cow age within year, and random animal effects. Heterosis estimates for Romosinuano-Brahman calving and weaning rate were 0.06 +/- 0.02 and 0.07 +/- 0.03 (P < 0.05); those for Brahman-Angus were twice as large (0.13 +/- 0.03 and 0.14 +/- 0.03, respectively; P < 0.001). Estimates of Brahman direct effects on calving and weaning rate were -0.12 +/- 0.04 and -0.14 +/- 0.05 (P < 0.05); however, Angus direct effects were beneficial for both traits (0.1 +/- 0.05, P < 0.05). The effect of heterosis was to reduce calving interval by -49.2 +/- 9.9 and -37.2 +/- 9.7 d for Romosinuano-Brahman and Brahman-Angus, respectively (P < 0.001). Romosinuano and F-1 cows sired by Romosinuano and out of Angus dams had the most occurrences of difficult births as a proportion of cows that calved (0.028 and 0.025, P = 0.04). Angus-sired crossbred cows and Brahman cows had the most occurrences of udder problems as a proportion of lactating cows (0.14 to 0.21, P < 0.04). There were more Brahman-sired cows that died or were culled as a proportion of those cows that began the project (0.1 to 0.28, P < 0.02) than cows in the other breed groups. Romosinuano-Brahman and Romosinuano-Angus cow performance was acceptable, but for most traits, those pairs of breeds had lower heterosis than Brahman-Angus. C1 [Riley, D. G.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Chase, C. C., Jr.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Coleman, S. W.] ARS, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. [Olson, T. A.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. RP Riley, DG (reprint author), Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. EM david-riley@tamu.edu NR 46 TC 2 Z9 2 U1 1 U2 11 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2014 VL 92 IS 5 BP 1902 EP 1910 DI 10.2527/jas.2013-7279 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH0SM UT WOS:000335830600010 PM 24663198 ER PT J AU Riley, DG Chase, CC Coleman, SW Olson, TA AF Riley, D. G. Chase, C. C., Jr. Coleman, S. W. Olson, T. A. TI Evaluation of the Criollo breed Romosinuano as purebred and crossbred cows with Brahman and Angus in Florida. II. Maternal influence on calf traits, cow weight, and measures of maternal efficiency SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE Brahman; crossbreeding; heterosis; maternal; Romosinuano ID BOS INDICUS BREEDS; BEEF-CATTLE; PREWEANING TRAITS; INDIVIDUAL TRAITS; SIMMENTAL CATTLE; GROWTH TRAITS; HETEROSIS; HEREFORD; PERFORMANCE; CROSSES AB The objectives of this work were to compare the Criollo breed Romosinuano as straightbred and crossbred cows with Angus and Brahman in subtropical Florida and to estimate heterosis for size traits of their calves, their own weight, and maternal efficiency traits. Cows (n = 404) were born from 2002 to 2005. After their first exposure to bulls as young cows, crossbred cows were bred to bulls of the third breed, and straightbred cows were bred in to bulls of the other 2 breeds. Calves were spring-born from 2005 through 2011. Evaluated calf (n = 1,254) traits included birth weight and weight, ADG, BCS, and hip height at weaning. Cow weight (n = 1,389) was recorded at weaning. Maternal efficiency traits evaluated included weaning weight per 100 kg cow weight, weaning weight per calving interval, and weaning weight per cow exposed to breeding (n = 1,442). Fixed effects and their interactions were investigated included sire and dam breed of cow, sire breed of calf, cow age, year, calf gender, and weaning age as a linear covariate (calf traits at weaning). Direct and maternal additive genetic effects were random in models for calf traits; only direct additive effects were modeled for cow traits. Cows sired by Angus bulls from outside the research herd had calves that were heavier at birth and weaning and greater ADG, BCS, and hip height (P < 0.05). Estimates of heterosis for weaning weight, BCS, and ADG ranged from 1.3 to 13.2% for all pairs of breeds (P < 0.05). Estimates of heterosis for birth weight (3.2 to 8.2%) and hip height (2.3%) were significant for Romosinuano-Angus and Brahman-Angus. Heterosis for cow weight was 65 +/- 8 kg for Brahman-Angus (P < 0.001), and estimates for other pairs of breeds were approximately one-half that value. Heterosis for weaning weight/100 kg cow weight was 3.4 +/- 0.75 kg for Romosinuano-Angus. Heterosis estimates for weaning weight/calving interval (P < 0.001) ranged from 0.08 +/- 0.01 to 0.12 +/- 0.01. Heterosis for weaning weight/cow exposed were 31.6 7.7, 36.9 +/- 7.4, and 59.1 +/- 7.5 kg for Romosinuano-Angus, Romosinuano-Brahman, and Brahman-Angus, respectively (P < 0.001). Most aspects of Romosinuano crossbred maternal performance were acceptable; maternal performance of Brahman-Angus cows excelled. C1 [Riley, D. G.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Chase, C. C., Jr.] Agr Res Serv, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Coleman, S. W.] Agr Res Serv, USDA, Grazinglands Res Lab, El Reno, OK 73036 USA. [Olson, T. A.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. RP Riley, DG (reprint author), Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. EM david-riley@tamu.edu NR 34 TC 3 Z9 3 U1 2 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2014 VL 92 IS 5 BP 1911 EP 1919 DI 10.2527/jas.2013-7280 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH0SM UT WOS:000335830600011 PM 24671581 ER PT J AU McDaneld, TG Kuehn, LA Thomas, MG Snelling, WM Smith, TPL Pollak, EJ Cole, JB Keele, JW AF McDaneld, T. G. Kuehn, L. A. Thomas, M. G. Snelling, W. M. Smith, T. P. L. Pollak, E. J. Cole, J. B. Keele, J. W. TI Genomewide association study of reproductive efficiency in female cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE bovine; genomewide association study; pooling; reproductive efficiency ID 1ST SERVICE CONCEPTION; FALSE DISCOVERY RATE; WIDE ASSOCIATION; BRANGUS HEIFERS; DNA POOLS; TRAITS; EMBRYOGENESIS; PROLIFERATION; HERITABILITY; FERTILITY AB Reproductive efficiency is of economic importance in commercial beef cattle production, as failure to achieve pregnancy reduces the number of calves marketed per cow exposed. Identification of genetic markers with predictive merit for reproductive success would facilitate early selection of sires with daughters having improved reproductive rate without increasing generation intervals. To identify regions of the genome harboring variation affecting reproductive success, we applied a genomewide association study (GWAS) approach based on the >700,000 SNP marker assay, using a procedure based on genotyping multianimal pools of DNA to increase the number of animals that could be genotyped with available resources. Cows from several populations were classified according to reproductive efficiency, and DNA was pooled within population and phenotype prior to genotyping. Populations evaluated included a research population at the U.S. Meat Animal Research Center, 2 large commercial ranch populations, and a number of smaller populations (<100 head) across the United States. We detected 2 SNP with significant genomewide association (P <= 1.49 x 10(-7)), on BTA21 and BTA29, 3 SNP with suggestive associations (P <= 2.91 x 10(-6)) on BTA5, and 1 SNP with suggestive association each on BTA1 and BTA25. In addition to our novel findings, we confirmed previously published associations for SNP on BTA-X and all autosomes except 3 (BTA21, BTA22, and BTA28) encompassing substantial breed diversity including Bos indicus and Bos taurus breeds. The study identified regions of the genome associated with reproductive efficiency, which are being targeted for further analysis to develop robust marker systems, and demonstrated that DNA pooling can be used to substantially reduce the cost of GWAS in cattle. C1 [McDaneld, T. G.; Kuehn, L. A.; Snelling, W. M.; Smith, T. P. L.; Pollak, E. J.; Keele, J. W.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Thomas, M. G.] Colorado State Univ, Dept Anim Sci, Ft Collins, CO 80523 USA. [Cole, J. B.] Agr Res Serv, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. RP McDaneld, TG (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM tara.mcdaneld@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU USDA-AFRI [2008-35205-18751] FX Financial support provided in part by USDA-AFRI (grant no. 2008-35205-18751) for collection of DNA samples. NR 47 TC 7 Z9 7 U1 0 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2014 VL 92 IS 5 BP 1945 EP 1957 DI 10.2527/jas.2012-6807 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH0SM UT WOS:000335830600015 PM 24782394 ER PT J AU Notter, DR Mousel, MR Leeds, TD Zerby, HN Moeller, SJ Lewis, GS Taylor, JB AF Notter, D. R. Mousel, M. R. Leeds, T. D. Zerby, H. N. Moeller, S. J. Lewis, G. S. Taylor, J. B. TI Evaluation of Columbia, U. S. Meat Animal Research Center Composite, Suffolk, and Texel rams as terminal sires in an extensive rangeland production system: VI. Measurements of live-lamb and carcass shape and their relationship to carcass yield and value SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE breed; carcass; linear measurements; prediction equations; shape; sheep ID USMARC-COMPOSITE; VISION SYSTEM; SHEEP; MUSCULARITY; QUALITY; CONFORMATION; VALIDATION; ACCURACY; WEIGHTS; TRAITS AB Linear measurements on live lambs and carcasses can be used to characterize sheep breeds and may have value for prediction of carcass yield and value. This study used 512 crossbred lambs produced over 3 yr by mating Columbia, U.S. Meat Animal Research Center (USMARC) Composite, Suffolk, and Texel rams to adult Rambouillet ewes to assess sire-breed differences in live-animal and carcass shape and to evaluate the value of shape measurements as predictors of chilled carcass weight (CCW), weight of high-value cuts (rack, loin, leg, and sirloin; HVW), weight of trimmed high-value cuts (trimmed rack and loin and trimmed, boneless leg and sirloin; TrHVW), and estimated carcass value before (CVal) and after trimming of high-value cuts (TrCVal). Lambs were produced under extensive rangeland conditions, weaned at an average age of 132 d, fed a concentrate diet in a drylot, and harvested in each year in 3 groups at target mean BW of 54, 61, and 68 kg. Canonical discriminant analysis indicated that over 93% of variation among sire breeds was accounted for by the contrast between tall, long, less-thickly muscled breeds with greater BW and CCW (i.e., the Columbia and Suffolk) compared with shorter, more thickly muscled breeds with smaller BW and CCW. After correcting for effects of year, harvest group, sire breed, and shipping BW, linear measurements on live lambs contributed little to prediction of CCW. Similarly, after accounting for effects of CCW, linear measurements on live animals further reduced residual SD (RSD) of dependent variables by 0.2 to 5.7%, with generally positive effects of increasing live leg width and generally negative effects of increasing heart girth. Carcass measurements were somewhat more valuable as predictors of carcass merit. After fitting effects of CCW, additional consideration of carcass shape reduced RSD by 2.1, 3.6, 9.5, and 2.2% for HVW, TrHVW, CVal, and TrCVal, respectively. Effects of increasing carcass leg width were positive for HVW, TrHVW, and TrCVal. We also observed positive effects of increasing carcass length on TrCVal and negative effects of increasing cannon bone length on HVW and CVal. Increasing shoulder width had positive effects on CVal but negative effects on TrHVW. Differences in lamb and carcass shape were significantly associated with carcass yield and value, but the additional accuracy associated with use of these measurements was modest relative to that achieved from use of only shipping BW or CCW. C1 [Notter, D. R.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Mousel, M. R.; Leeds, T. D.; Lewis, G. S.; Taylor, J. B.] ARS, USDA, US Sheep Expt Stn, Dubois, ID 83423 USA. [Zerby, H. N.; Moeller, S. J.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. RP Notter, DR (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM drnotter@vt.edu NR 24 TC 1 Z9 1 U1 0 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2014 VL 92 IS 5 BP 1980 EP 1994 DI 10.2527/jas.2013-7154 PG 15 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH0SM UT WOS:000335830600018 PM 24663175 ER PT J AU Bansal, S James, JJ Sheley, RL AF Bansal, Sheel James, Jeremy J. Sheley, Roger L. TI The effects of precipitation and soil type on three invasive annual grasses in the western United States SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Bromus tectorum; Cheatgrass; Climate change; Medusahead; Precipitation; Soil texture; Ventenata dubia ID BROMUS-TECTORUM L; TEXTURE; CHEATGRASS; DESERT; PULSE; UTAH AB Multiple species of annual grasses are invading sagebrush-steppe communities throughout the western United States. Most research has focused on dominant species such as Bromus tectorum (cheatgrass), yet other, less studied annual grasses such as Taeniatherum caput-medusae (medusahead) and Ventenata dubia (ventenata) are spreading rapidly. Future precipitation regimes are expected to have less frequent but more intense rain events, which may affect soil moisture availability and favor these 'newer' invasives over cheatgrass. We conducted a full factorial, growth chamber study examining the effects of two watering regimes (small/frequent, large/infrequent rain pulses) across nine soil types on the growth of cheatgrass, medusahead and ventenata. We tested a hypothesis that medusahead or ventenata would have greater growth than cheatgrass with larger/infrequent rain events. The two watering regimes had relatively strong effects on soil water content, but generally did not impact plant growth. In contrast, variation in soil properties such as clay content, pH and soil N correlated with a two-to four-fold change in plant growth. The three invasive grass species generally respond similarly to changes in precipitation regimes and to edaphic factors. Nevertheless, medusahead had 30-40% overall greater root growth compared to the other species and a 15% increase in root growth in response to the large/infrequent watering treatment. Our findings reveal that 1) greater biomass allocation to roots and 2) greater responsiveness of root growth to differing precipitation regimes of medusahead may favor its ecological success over other invasive annuals under future climate scenarios. Published by Elsevier Ltd. C1 [Bansal, Sheel; James, Jeremy J.; Sheley, Roger L.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Bansal, S (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Olympia Forestry Sci Lab, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM sheelbansa19@gmail.com; jjjames@ucanr.edu; roger.sheley@oregonstate.edu OI Bansal, Sheel/0000-0003-1233-1707 FU USDA-Agricultural Research Service Areawide Project for Ecologically-based Invasive Plant Management of Annual Grasses in the Great Basin Ecosystem FX We like to thank Brett Bingham for assistance with soil collection and analysis. This research was funded through the USDA-Agricultural Research Service Areawide Project for Ecologically-based Invasive Plant Management of Annual Grasses in the Great Basin Ecosystem. NR 20 TC 5 Z9 5 U1 8 U2 69 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAY PY 2014 VL 104 BP 38 EP 42 DI 10.1016/j.jaridenv.2014.01.010 PG 5 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH1IE UT WOS:000335873000006 ER PT J AU Augustine, DJ Brewer, P Blumenthal, DM Derner, JD von Fischer, JC AF Augustine, David J. Brewer, Paul Blumenthal, Dana M. Derner, Justin D. von Fischer, Joseph C. TI Prescribed fire, soil inorganic nitrogen dynamics, and plant responses in a semiarid grassland SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Ammonium; Nitrate; Nitrogen cycling; Plant invasion; Rangeland management; Soil moisture ID SHORTGRASS STEPPE; TALLGRASS PRAIRIE; BROMUS-TECTORUM; NORTH-AMERICA; WATER PULSES; LONG-TERM; AVAILABILITY; ECOSYSTEMS; RANGELAND; GRASSES AB In arid and semiarid ecosystems, fire can potentially affect ecosystem dynamics through changes in soil moisture, temperature, and nitrogen cycling, as well as through direct effects on plant meristem mortality. We examined effects of annual and triennial prescribed fires conducted in early spring on soil moisture, temperature, and N, plant growth, and plant N content in semiarid shortgrass steppe. Annual burning increased soil inorganic N availability throughout the growing season, which was associated with increased soil temperature and a reduction in aboveground N in C-3 plants. Furthermore, the increase in soil inorganic N pools with annual burning was modest and did not facilitate success of ruderal species. Negative fire effects on C-3 plant production could be due to increased soil temperature, reduced soil moisture, or direct negative effects on C-3 plant meristems, although fuel loads and fire temperatures were low relative to other grasslands. Triennial burning had intermediate effects on N availability and C-3 plant production compared to annual burning and unburned controls. Results show that prescribed burns can be used in the management of this semiarid grassland without facilitating annual plant invasion, but excessively frequent burning can reduce production of C-3 plants. Published by Elsevier Ltd. C1 [Augustine, David J.; Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80525 USA. [Brewer, Paul; von Fischer, Joseph C.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Brewer, Paul; von Fischer, Joseph C.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. RP Augustine, DJ (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80525 USA. EM David.Augustine@ars.usda.gov OI von Fischer, Joseph/0000-0001-5508-6073; Brewer, Paul/0000-0002-2715-5286 NR 51 TC 8 Z9 9 U1 6 U2 53 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAY PY 2014 VL 104 BP 59 EP 66 DI 10.1016/j.jaridenv.2014.01.022 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH1IE UT WOS:000335873000009 ER PT J AU Sheley, RL James, JJ AF Sheley, Roger L. James, Jeremy J. TI Simultaneous intraspecific facilitation and interspecific competition between native and annual grasses SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Annual grass; Drought; Invasion; Plant size effects; Plant strategies ID TAENIATHERUM-CAPUT-MEDUSAE; RELATIVE GROWTH-RATE; PLANT-COMMUNITIES; POSITIVE INTERACTIONS; FIELD EXPERIMENTS; LIFE-HISTORY; LEAF-AREA; INVASION; SIZE; GRASSLAND AB Invasive annual grasses tend to construct thinner and less dense root and leaf tissue than native perennial grasses. This allows invasive annuals to grow faster and produce more biomass in the arid grasslands of the United States. Based on these differences we tested the hypotheses that: 1) Competitive effects of the native perennial on the invasive annual will increase as plant developmental stage increases and as drought stress increases. 2) Drought stress will reduce the competitive effect of invasive annuals on native perennials proportionately more than drought stress reduces the competitive effect of native perennials on the invasive annual. 3) Facilitation among native perennial grass seedlings will decrease as developmental stage increases. Competitive effects of native squirreltail, on invasive medusahead, increased as the initial developmental stage of squirreltail increased, but not vice versa. Drought stress reduced the competitive effect of medusahead on squirreltail target biomass more than drought stress reduced the competitive effect of squirreltail on medusahead target biomass. While both squirreltail and medusahead displayed intraspecific facilitation, the net effect of their interspecific interaction was negative for both species. Habitat amelioration manifests itself differently depending on species traits, and can create conditions that simultaneously benefit one species while hindering another. Published by Elsevier Ltd. C1 [Sheley, Roger L.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [James, Jeremy J.] Univ Calif, Sierra Foothill Res & Extens Ctr, Browns Valley, CA 95918 USA. RP Sheley, RL (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A,Hwy 205, Burns, OR 97720 USA. EM roger.sheley@oregonstate.edu NR 85 TC 3 Z9 3 U1 8 U2 125 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAY PY 2014 VL 104 BP 80 EP 87 DI 10.1016/j.jaridenv.2014.01.019 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AH1IE UT WOS:000335873000011 ER PT J AU Ren, H Guo, QF Liu, H Li, J Zhang, QM Xu, HL Xu, FH AF Ren, Hai Guo, Qinfeng Liu, Hong Li, Jing Zhang, Qianmei Xu, Hualin Xu, Fanghong TI Patterns of Alien Plant Invasion across Coastal Bay Areas in Southern China SO JOURNAL OF COASTAL RESEARCH LA English DT Article DE Alien species; mangroves; coastal zone; China; Guangdong; Shenzhen Bay ID SONNERATIA-APETALA; MANGROVE FOREST; RESTORATION; PLANTATIONS; ECOSYSTEMS; VEGETATION; GRADIENTS; IMPACTS AB An understanding of the ways in which levels of invasions by alien species are correlated with environmental factors is helpful to manage the negative impacts of these invasive species. Two tropical coastal areas in South China, Shenzhen Bay and Leizhou Bay, are national nature reserves, but they are threatened by invasive plants. Here, we investigated the level of invasion by exotic plants at both bays, and its relationship with selected environmental factors. We found a total of 34 invasive plant species, 18 of which were present at both bays; among these, 15 species were in terrestrial areas, three were at the ecotone, and one was in the mangroves. The two bays had a similar degree of invasion but were dominated by different species. Three invasive species (Ipomoea purpurea, Wedelia trilobata, and Panicum repens) were abundant at both bays, and only one species, Sonneratia apetala, was present in the mangroves. The number of alien species increased from mangrove to ecotone to terrestrial areas in both bays, while, in proportion, ecotone supported the most alien species in Shenzhen Bay. The relationships between plant invasion and habitat features depended on the variables that were used to measure the degree of invasions. In general, the occurrence of alien species was positively correlated with soil organic carbon and total N content but negatively correlated with the leaf area index and soil salinity. The biomass of alien plants was, on the other hand, positively correlated with total N and soil water content in the soil, and the density of alien plants was not correlated with habitat characteristics. Most of the alien plants originated from tropical America, but a few were from Africa and south Asia. We recommend restoration efforts that include removal of alien species, soil improvement, and the planting of native species. C1 [Ren, Hai; Li, Jing; Zhang, Qianmei] Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. [Guo, Qinfeng] US Forest Serv, So Res Stn, USDA, Asheville, NC 28804 USA. [Liu, Hong] Florida Int Univ, Dept Earth & Environm, Miami, FL 33199 USA. [Liu, Hong] Fairchild Trop Bot Garden, Miami, FL 33156 USA. [Xu, Hualin] Neilingding Futian Natl Nat Reserve Guangdong, Shenzhen 518040, Peoples R China. [Xu, Fanghong] Zhanjiang Mangrove Natl Nat Reserve Guangdong, Zhanjiang 524448, Peoples R China. RP Ren, H (reprint author), Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China. EM renhai@scbg.ac.cn FU National Natural Science Foundation of China [31170493]; Guangdong Program [2011B060400003] FX We thank Dr. Nan Liu and Jun Wang at South China Botanical Garden, and the staffs of the Administration of Futian and Zhanjiang Mangrove National Natural Reserve for field assistance. Thanks go to Dr. Baoming Chen for valuable comments. Thanks are also due to Prof. Bruce Jaffee for polishing the English. We also thank the anonymous reviewers for their valuable comments on an earlier version of the manuscript. The project was supported by the National Natural Science Foundation of China (31170493) and by the Guangdong Program (2011B060400003). NR 43 TC 0 Z9 2 U1 7 U2 31 PU COASTAL EDUCATION & RESEARCH FOUNDATION PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0749-0208 EI 1551-5036 J9 J COASTAL RES JI J. Coast. Res. PD MAY PY 2014 VL 30 IS 3 BP 448 EP 455 DI 10.2112/JCOASTRES-D-13-00096.1 PG 8 WC Environmental Sciences; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AH3EA UT WOS:000336005100003 ER PT J AU Augustine, DJ Skagen, SK AF Augustine, David J. Skagen, Susan K. TI Mountain Plover Nest Survival in Relation to Prairie Dog and Fire Dynamics in Shortgrass Steppe SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE black-tailed prairie dogs; Cynomys ludovicianus; disturbance regimes; grassland birds; prescribed fire; rangeland management; spatial heterogeneity; vegetation structure ID GREAT-PLAINS; CYNOMYS-LUDOVICIANUS; CHARADRIUS-MONTANUS; ATHENE-CUNICULARIA; GRASSLAND BIRDS; NORTH-AMERICA; PLAGUE; CONSERVATION; RESPONSES; HABITAT AB Disturbed xeric grasslands with short, sparse vegetation provide breeding habitat for mountain plovers (Charadrius montanus) across the western Great Plains. Maintaining local disturbance regimes through prairie dog conservation and prescribed fire may contribute to the sustainability of recently declining mountain plover populations, but these management approaches can be controversial. We estimated habitat-specific mountain plover densities and nest survival rates on black-tailed prairie dog (Cynomys ludovicianus) colonies and burns in the shortgrass steppe of northeastern Colorado. Mountain plover densities were similar on prairie dog colonies (5.9 birds/km(2); 95% CI = 4.7-7.4) and sites burned during the preceding dormant season (6.7 birds/km(2); 95% CI = 4.6-9.6), whereas the 29-day nest survival rate was greater on prairie dog colonies (0.81 in 2011 and 0.39 in 2012) compared to the burned sites (0.64 in 2011 and 0.17 in 2012). Reduced nest survival in 2012 compared to 2011 was associated with higher maximum daily temperatures in 2012, consistent with a previous weather-based model of mountain plover nest survival in the southern Great Plains. Measurements of mountain plover density relative to time since disturbance showed that removal of prairie dog disturbance by sylvatic plague reduced mountain plover density by 70% relative to active prairie dog colonies after 1 year. Plover densities declined at a similar rate (by 78%) at burned sites between the first and second post-burn growing season. Results indicate that black-tailed prairie dog colonies are a particularly important nesting habitat for mountain plovers in the southern Great Plains. In addition, findings suggest that prescribed burning can be a valuable means to create nesting habitat in landscapes where other types of disturbances (such as prairie dog colonies) are limited in distribution and size. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Augustine, David J.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Skagen, Susan K.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. RP Augustine, DJ (reprint author), ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM david.augustine@ars.usda.gov FU United States Department of Agriculture's-Agricultural Research Service FX Funding for this study was provided by the United States Department of Agriculture's-Agricultural Research Service. We thank P. McCusker, E. Klarquist, J. Baker, C. Kennedy, and K. Earixon for their assistance finding and monitoring nests, and P. McCusker for conducting point count surveys. We thank R. Reichert, E. Humphrey, K. Philbrook, and L. Bell for facilitating our work on the Pawnee National Grassland, and F. Knopf for advice and insight while initiating the study. We thank 2 anonymous reviewers, W. Block, and E. Merrill for many helpful comments and suggestions. The USDA-ARS is an equal opportunity/affirmative action employer, and all agency services are available without discrimination. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 51 TC 3 Z9 4 U1 8 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2014 VL 78 IS 4 BP 595 EP 602 DI 10.1002/jwmg.700 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AH3MO UT WOS:000336028100004 ER PT J AU Wengert, GM Gabriel, MW Matthews, SM Higley, JM Sweitzer, RA Thompson, CM Purcell, KL Barrett, RH Woods, LW Green, RE Keller, SM Gaffney, PM Jones, M Sacks, BN AF Wengert, Greta M. Gabriel, Mourad W. Matthews, Sean M. Higley, J. Mark Sweitzer, Rick A. Thompson, Craig M. Purcell, Kathryn L. Barrett, Reginald H. Woods, Leslie W. Green, Rebecca E. Keller, Stefan M. Gaffney, Patricia M. Jones, Megan Sacks, Benjamin N. TI Using DNA to Describe and Quantify Interspecific Killing of Fishers in California SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE bobcat; Canis latrans; coyote; fisher; interspecific killing; intraguild predation; Lynx rufus; Martes pennanti; mountain lion; Pekania pennanti; predator; Puma concolor ID JOAQUIN KIT FOXES; INTRAGUILD PREDATION; MOUNTAIN LIONS; NATIONAL-PARK; ISLAND FOX; CARNIVORES; EXTINCTION; COYOTES; DECLINE; PREY AB Interspecific killing is common among carnivores and can have population-level effects on imperiled species. The fisher (Pekania [ Martes] pennanti) is a rare forest carnivore in western North America and a candidate for listing under the United States Endangered Species Act. Interspecific killing and intraguild predation are poorly understood in fishers and potential threats to existing western populations. We studied the prevalence and patterns of interspecific killing of fishers in the southern Sierra Nevada and Coastal Range of California. We collected forensic evidence and samples from the carcasses and predation sites, conducted full necropsies when possible, and used molecular methods to determine species of predators responsible for killing fishers. We recovered 101 (59 female, 42 male) fisher carcasses; for 62 (61%) carcasses, we attributed cause of death to interspecific killing. We found that bobcats (Lynx rufus, n = 25 fisher mortalities), mountain lions (Puma concolor, n = 20), and coyotes (Canis latrans, n = 4) were predators of fishers in our study areas. Bobcats killed only female fishers, whereas mountain lions more frequently killed male than female fishers, confirming our hypothesis that female fishers would suffer lethal attacks by smaller predators than would male fishers. Coyotes rarely killed fishers. We found differences in pathologic characteristics of the predation events among the 3 predator species, which may be helpful in identifying predator species. (C) 2014 The Wildlife Society. C1 [Wengert, Greta M.; Sacks, Benjamin N.] Univ Calif Davis, Vet Genet Lab, Davis, CA 95616 USA. [Wengert, Greta M.; Gabriel, Mourad W.] Integral Ecol Res Ctr, Blue Lake, CA 95525 USA. [Matthews, Sean M.] Wildlife Conservat Soc, Hoopa, CA 95546 USA. [Higley, J. Mark] Hoopa Tribal Forestry, Dept Wildlife, Hoopa, CA 95546 USA. [Sweitzer, Rick A.; Barrett, Reginald H.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Thompson, Craig M.; Purcell, Kathryn L.; Green, Rebecca E.] US Forest Serv, USDA, Pacific Southwest Res Stn, Fresno, CA 93710 USA. [Woods, Leslie W.] Calif Anim Hlth & Food Safety Lab Syst, Davis, CA 95616 USA. [Keller, Stefan M.; Gaffney, Patricia M.; Jones, Megan] Univ Calif Davis, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. RP Wengert, GM (reprint author), Univ Calif Davis, Vet Genet Lab, Davis, CA 95616 USA. EM gmwengert@ucdavis.edu RI Purcell, Kathryn/S-2592-2016 FU Integral Ecology Research Center; Hoopa Valley Tribe; United States Forest Service, Pacific Southwest Research Station; Sierra Nevada Adaptive Management Project; U.S. Department of Agriculture [USDA] Forest Service Region 5; USDA Forest Service Pacific Southwest Research Station; U.S. Fish and Wildlife Service; California Department of Water Resources; California Department of Fish and Wildlife; California Department of Forestry and Fire Protection; Sierra Nevada Conservancy; University of California [U.C.] Davis Veterinary Medical Teaching Hospital; California Animal Health and Food Safety Laboratory; United States Fish and Wildlife Service, Tribal Wildlife Grant Program; United States Department of the Interior Bureau of Indian Affairs; U.C. Davis Veterinary Genetics Laboratory, Canid Diversity and Conservation Unit; U.C. Davis Foley Laboratory in Infectious Disease Ecology; U.C. Davis Center for Population Biology; Wildlife Conservation Society FX Funding and logistical support for this research was provided by Integral Ecology Research Center; Hoopa Valley Tribe; United States Forest Service, Pacific Southwest Research Station; Sierra Nevada Adaptive Management Project (funded by U.S. Department of Agriculture [USDA] Forest Service Region 5, USDA Forest Service Pacific Southwest Research Station, U.S. Fish and Wildlife Service, California Department of Water Resources, California Department of Fish and Wildlife, California Department of Forestry and Fire Protection, and the Sierra Nevada Conservancy); California Department of Fish and Wildlife; University of California [U.C.] Davis Veterinary Medical Teaching Hospital; California Animal Health and Food Safety Laboratory; United States Fish and Wildlife Service, Tribal Wildlife Grant Program; United States Department of the Interior Bureau of Indian Affairs; U.C. Davis Veterinary Genetics Laboratory, Canid Diversity and Conservation Unit; U.C. Davis Foley Laboratory in Infectious Disease Ecology; U.C. Davis Center for Population Biology; and the Wildlife Conservation Society. We thank the field crews at the Hoopa Valley Indian Reservation Fisher Project, Sierra Nevada Adaptive Management Project, and Kings River Fisher Project for their tireless efforts to retrieve fisher carcasses and collect pertinent information on fisher predation events, and laboratory interns at Integral Ecology Research Center and U.C. Davis Veterinary Genetics Laboratory for assistance with molecular analyses. Thanks to the other members of my dissertation committee, Dr. A. Sih and Dr. R. Hijmans, for insightful reviews of this manuscript. We give special thanks to the late Dr. L. Munson, who contributed immensely to early necropsies and to our better understanding of the pathology associated with mortality caused by predation. NR 50 TC 11 Z9 11 U1 5 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2014 VL 78 IS 4 BP 603 EP 611 DI 10.1002/jwmg.698 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AH3MO UT WOS:000336028100005 ER PT J AU Lee, SH Lillehoj, HS Jang, SI Jeong, MS Xu, SZ Kim, JB Park, HJ Kim, HR Lillehoj, EP Bravo, DM AF Lee, S. H. Lillehoj, H. S. Jang, S. I. Jeong, M. S. Xu, S. Z. Kim, J. B. Park, H. J. Kim, H. R. Lillehoj, E. P. Bravo, D. M. TI Effects of in ovo injection with selenium on immune and antioxidant responses during experimental necrotic enteritis in broiler chickens SO POULTRY SCIENCE LA English DT Article DE selenium; chicken; antioxidant; immunology; necrotic enteritis ID MESSENGER-RNA EXPRESSION; EIMERIA-MAXIMA INFECTION; CLOSTRIDIUM-PERFRINGENS; GENE-EXPRESSION; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; PATHOGENESIS; COCCIDIOSIS; SUSCEPTIBILITY; VACCINATION AB This study was conducted to investigate the effects of in ovo injection of Se on modulating the immune system and antioxidant responses in broiler chickens with experimental necrotic enteritis. Broiler eggs were injected at 18 d of embryo age with either 100 mu L of PBS alone or sodium selenite (Na2SeO3) in PBS, providing 0 (SS0), 10 (SS10), or 20 (SS20) mu g of Se/egg. At 14 d posthatch, PBS-treated and uninfected chickens were kept as the control group, whereas the remaining chickens were orally infected with 1.0 x 10(4) sporulated oocysts of Eimeria maxima (SS0, SS10, SS20). At 18 d posthatch, E. maxima-infected chickens were orally infected with 1.0 x 10(9) cfu of Clostridium perfringens. Infected control SS0 group showed significantly decreased BW compared with the uninfected control. However, SS20 group showed significantly increased BW compared with the infected control SS0 group, whereas the BW were similar among uninfected control and infected SS10 and SS20 groups. The SS10 group showed significantly lower intestinal lesions compared with the SS0 group, and oocyst production was decreased in both SS10 and SS20 groups. Serum malondialdehyde level and catalase activity were also decreased in both SS10 and SS20 groups, whereas the superoxide dismutase level was significantly lower in the SS10 group compared with the SS0 group. The SS20 group showed significantly higher levels of transcripts for IL-1 beta and IL-6 in intestine, and SS10 and SS20 groups had higher levels of transcripts for IL-8 and inducible nitric oxide synthase expression and decreased glutathione peroxidase 7 mRNA levels compared with the SS0 group. The SS10 and SS20 groups also showed increased serum antibody levels to C. perfringens a-toxin and NetB toxin compared with the SS0 group. These collective results suggest that the injection of Se into the amniotic cavity of developing eggs may be beneficial for enhancing immune and antioxidant responses in the hatched chickens exposed to the necrotic enteritis-causing pathogens. C1 [Lee, S. H.; Lillehoj, H. S.; Jang, S. I.; Jeong, M. S.; Xu, S. Z.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Lee, S. H.; Kim, J. B.; Park, H. J.; Kim, H. R.] Rural Dev Adm, Natl Acad Agr Sci, Suwon 445853, Gyeonggi, South Korea. [Xu, S. Z.] Qingdao Agr Univ, Coll Anim Sci & Vet Med, Qingdao 266109, Peoples R China. [Lillehoj, E. P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. [Bravo, D. M.] Pancosma SA, CH-1218 Geneva, Switzerland. RP Lillehoj, HS (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU Agricultural Research Service-USDA; Pancosma S.A.; project "Study on Flavonoids Data Base Construction from Agri-Foods of Rural Development Administration, Republic of Korea [PJ010056] FX This study was partially supported by a formal trust agreement established between Agricultural Research Service-USDA and Pancosma S.A. and by the project "Study on Flavonoids Data Base Construction from Agri-Foods (PJ010056)" of Rural Development Administration, Republic of Korea. The authors thank Kyung Woo Lee, Duk Kyung Kim, Myeong Seon Park, Marjorie Nichols, Stacy O'Donnell, and Heng Tech (Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, USDA-ARS, Beltsville, MD) for scientific input and technical assistance. NR 38 TC 6 Z9 6 U1 2 U2 19 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2014 VL 93 IS 5 BP 1113 EP 1121 DI 10.3382/ps.2013-03770 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH2RY UT WOS:000335970000009 PM 24795303 ER PT J AU Su, S Miska, KB Fetterer, RH Jenkins, MC Wong, EA AF Su, S. Miska, K. B. Fetterer, R. H. Jenkins, M. C. Wong, E. A. TI Expression of digestive enzymes and nutrient transporters in Eimeria acervulina-challenged layers and broilers SO POULTRY SCIENCE LA English DT Article DE Eimeria acervulina; broiler; layer; nutrient transporter; liver expressed antimicrobial peptide 2 ID MAXIMA-INFECTED CHICKENS; GLUTAMATE TRANSPORTERS; ANTIMICROBIAL PEPTIDE; SMALL-INTESTINE; FOOD-INTAKE; MEMBRANE; RESPONSES; FAMILY; GROWTH; CELLS AB Avian coccidiosis is a disease caused by intestinal protozoa in the genus Eimeria. Clinical signs of coccidiosis include intestinal lesions and reduced feed efficiency and BW gain. This growth reduction may be due to changes in expression of digestive enzymes and nutrient transporters in the intestine. The objective of this study was to examine the differential expression of digestive enzymes, transporters of amino acids, peptides, sugars, and minerals, and an antimicrobial peptide in the small intestine of Eimeria acervulina-infected broilers and layers. Uninfected broilers and layers, in general, expressed these genes at comparable levels. Some differences included 3-fold and 2-fold greater expression of the peptide transporter PepT1 and the antimicrobial peptide LEAP2 (liver expressed antimicrobial peptide 2), respectively, in the jejunum of layers compared with broilers and 17-fold greater expression of LEAP2 in the duodenum of broilers compared with layers. In the duodenum of Eimeria-infected broilers and layers, there was downregulation of aminopepti-dase N; sucrase-isomaltase; the neutral, cationic, and anionic amino acid transporters b(o,)+ AT/rBAT, B(o)AT, CAT2, and EAAT3; the sugar transporter GLUT2; the zinc transporter ZnT1; and LEAP2. In the jejunum of infected layers there was downregulation of many of the same genes as in the duodenum plus downregulation of PepT1, b(o,)+ AT/rBAT, and the y(+) L system amino acid transporters y+ LAT1 and y(+) LAT2. In the ileum of infected layers there was downregulation of CAT2, y(+) LAT1, the L type amino acid transporter LAT1, and the sugar transporter GLUT1, and upregulation of APN, PepT1, the sodium glucose transporter SGLT4, and LEAP2. In E. acervulina-infected broilers, there were no gene expression changes in the jejunum and ileum. These changes in intestinal digestive enzyme and nutrient transporter gene expression may result in a decrease in the efficiency of protein digestion, uptake of important amino acids and sugars, and disruption of mineral balance that may affect intestinal cell metabolism and Eimeria replication. C1 [Su, S.; Wong, E. A.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Miska, K. B.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Fetterer, R. H.; Jenkins, M. C.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Wong, EA (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM ewong@vt.edu NR 32 TC 9 Z9 9 U1 3 U2 17 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2014 VL 93 IS 5 BP 1217 EP 1226 DI 10.3382/ps.2013-03807 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH2RY UT WOS:000335970000021 PM 24795315 ER PT J AU Jones, DR Karcher, DM Abdo, Z AF Jones, D. R. Karcher, D. M. Abdo, Z. TI Effect of a commercial housing system on egg quality during extended storage SO POULTRY SCIENCE LA English DT Article DE sustainable; egg production; housing; egg quality; storage ID LAYING HENS; FURNISHED CAGES; PRODUCTION PERFORMANCE; EGGSHELL STRENGTH; SHELL EGGS; STRAINS; TEMPERATURE; YOLK; KEPT AB Egg producers in the United States are utilizing a variety of commercial egg production systems to provide consumer choice and meet legislative requirements. Consumer egg grades in the United States were developed for conventional cage production, and it is unclear what effect alternative production systems might have on egg quality during retail and consumer home storage. The current study was undertaken to determine what changes in egg quality characteristics occur during extended cold storage for commercially produced conventional cage, enriched colony cage, and cage-free aviary eggs. During 12 wk of cold storage, egg weight, albumen height, Haugh unit, static compression shell strength, vitelline membrane strength and deformation, yolk index, shell dynamic stiffness, and whole egg total solids were monitored. Overall, aviary and enriched eggs were significantly (P < 0.05) heavier than conventional cage. Albumen height and Haugh unit (P < 0.05) were significantly greater for conventional cage than enriched eggs. Static compression shell strength was greatest (P < 0.05) for enriched eggs compared with aviary. No overall housing system effects for yolk measurements, shell dynamic stiffness, or whole egg total solids were observed. Albumen height, Haugh unit, and yolk quality measurements were all greatest at 0 and lowest at 12 wk of storage (P < 0.05). The rate of quality change among the housing systems for each measured attribute at 4, 6, and 12 wk was determined. Other than differences in the change of egg weight at 4 wk, no significant differences in the rate of quality decline were found among the housing systems. The results of the current study indicate that current US egg quality standards should effectively define quality for commercially produced conventional cage, enriched colony cage, and cage-free aviary eggs. C1 [Jones, D. R.] USDA ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. [Karcher, D. M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Abdo, Z.] USDA ARS, South Atlantic Area, Athens, GA 30605 USA. RP Jones, DR (reprint author), USDA ARS, Egg Safety & Qual Res Unit, Athens, GA 30605 USA. EM Deana.Jones@ars.usda.gov FU Coalition for a Sustainable Egg Supply (Kansas City, MO) FX Research support provided in part by a grant from the Coalition for a Sustainable Egg Supply (Kansas City, MO). NR 31 TC 15 Z9 15 U1 3 U2 37 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2014 VL 93 IS 5 BP 1282 EP 1288 DI 10.3382/ps.2013-03631 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA AH2RY UT WOS:000335970000030 PM 24795324 ER PT J AU Hall, DG Hentz, MG AF Hall, David G. Hentz, Matthew G. TI Asian Citrus Psyllid (Hemiptera: Liviidae) Tolerance to Heat SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE citrus greening; huanglongbing; Diaphorina citri ID CANDIDATUS LIBERIBACTER ASIATICUS; DIAPHORINA-CITRI; HOST-PLANT; TEMPERATURE; BIOLOGY; DISEASE; VECTOR; PARAMETERS; DROSOPHILA; HOMOPTERA AB The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important citrus pest because it vectors bacteria responsible for a serious citrus disease known as huanglongbing (also called citrus greening disease). We conducted research on Asian citrus psyllid heat tolerance, with special emphasis on identifying heat treatments lethal to adult Asian citrus psyllid. Working with an Asian citrus psyllid colony maintained at 27 degrees C, we assessed survival of adult Asian citrus psyllid exposed to temperatures of 43D60 degrees C for various durations in a laboratory oven. Wefound under our experimental conditions that adult Asian citrus psyllid cannot survive at 50 degrees C for more than 10 min and that death occurred faster at higher temperatures; males tended to be more heat-tolerant than females; young adults were less tolerant of high temperatures than older adults; adult Asian citrus psyllids predisposed to warmer temperatures were more heat-tolerant than those predisposed to cooler temperatures; and heat treatments lethal to adult Asian citrus psyllids were lethal to eggs and usually lethal to nymphs. Adult Asian citrus psyllids were less tolerant of heat treatments in the oven when the humidity level was high than when it was low. A number of heat treatments lethal to adult Asian citrus psyllid in an oven situation can be identified from the results of this research and would be useful to regulatory agencies interested in eliminating adult Asian citrus psyllids from shipments of citrus leaves destined for culinary or medicinal markets. Among a number of candidate treatments, 60 degrees C for at least 10 min would be effective against all Asian citrus psyllid life stages and faster killing than lower temperatures. C1 [Hall, David G.; Hentz, Matthew G.] USDA ARS, Ft Pierce, FL 34945 USA. RP Hall, DG (reprint author), USDA ARS, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM David.Hall@ars.usda.gov FU U.S. Department of Agriculturer Animal Plant Health Inspection ServiceDPlant Protection and Quarantine (USDADAPHISD PPQ) FX We acknowledge the following individuals for their contributions to this research: Charla Hollingsworth, Kayimbi Tubajika, Pat Gomes, and Mike Hennessey. Beth GraftonCardwell graciously reviewed an early draft of the manuscript, and we thank her for her helpful comments. This research was supported in-part by a grant from the U.S. Department of Agriculturer Animal Plant Health Inspection ServiceDPlant Protection and Quarantine (USDADAPHISD PPQ). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement. NR 24 TC 1 Z9 1 U1 2 U2 30 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2014 VL 107 IS 3 BP 641 EP 649 PG 9 WC Entomology SC Entomology GA AG8IS UT WOS:000335662600012 ER PT J AU Pearson, CC Backus, EA Shugart, HJ Munyaneza, JE AF Pearson, Cole C. Backus, Elaine A. Shugart, Holly J. Munyaneza, Joseph E. TI Characterization and Correlation of EPG Waveforms of Bactericera cockerelli (Hemiptera: Triozidae): Variability in Waveform Appearance in Relation to Applied Signal SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE zebra chip; Liberibacter; feeding behavior; potato psyllid; pathogen transmission ID CANDIDATUS LIBERIBACTER SOLANACEARUM; STYLET PENETRATION; PROBING BEHAVIOR; FEEDING-BEHAVIOR; PEAR PSYLLA; XYLELLA-FASTIDIOSA; DIAPHORINA-CITRI; FINE-STRUCTURE; ZEBRA CHIP; APHIDS AB The tomatoDpotato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), was recently shown to be a vector of " Candidatus Liberibacter solanacearum" (Lso), a phloem-limited bacterium that is the putative causal agent of " zebra chip" in potato and unnamed diseases in other solanaceous species. Despite its importance, very little is known about B. cockerelli stylet probing behaviors that control transmission of Lso to its host plants. Herein, we report therst study characterizing the electrical penetration graph waveforms representing stylet penetration behaviors of the B. cockerelli feeding on potato. Waveforms produced by adult B. cockerelli on potato were also correlated using light microscopy of salivary sheath termini in plant tissue after probes were arti_cially terminated during the identied waveforms. In addition, behavioral activities were inferred based on electrical origins of waveforms as well as similarities in waveform appearances with those of other psyllids, aphids, and whiteies. Adult B. cockerelli produced six waveform families and four types, which represent the following proposed biological meanings: family A, initial penetration and sheath salivation; family B, penetration of epidermal cells; family C, secretion of most of the salivary sheath and stylet pathway in mesophyll and parenchyma, with two types, C1 and C2, of unknown meaning; family D, initial contact with phloem cells; family E, activities in phloem cells, with two types, E1, putative phloem salivation, and E2, phloem sap ingestion; and family G, xylem ingestion. In addition, a previously unreported variant of waveform G was characterized and correlated with applied signal type. Variation in D and G waveform appearances was correlated with polarity, type, and magnitude of applied signal. Results suggest that active phloem sap ingestion during E2 may play a critical role in acquisition of Lso bacterial cells. E1 may be important in inoculation of the bacterium into phloem sieve elements because it may represent salivation into phloem sieve elements. Analysis of B. cockerelli waveforms could lead to faster development of resistant host plant varieties, strengthen integrated pest management strategies by incorporating alternative plant hosts, and maximize the efciency of pesticides. C1 [Pearson, Cole C.; Munyaneza, Joseph E.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Pearson, Cole C.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Backus, Elaine A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Shugart, Holly J.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. RP Munyaneza, JE (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM joseph.munyaneza@ARS.USDA.gov FU Frito Lay Inc.; USDADARS State Cooperative Potato Research Program, Texas Department of Agriculture; U. S. Department of AgricultureDNational Institute of Food and AgricultureDRisk Avoidance and Mitigation (USDADNIFADRAMP) [2009-51101-05892]; U. S. Department of AgricultureDNa-tional Institute of Food and AgricultureDThe Specialty Crop Research Initiative (USDADNIFADSCRI) [200951181- 20176] FX We thank Millie Heidt and Blaine Heilman for their technical assistance. We also appreciate the efforts of Timothy Ebert, University of Florida, Citrus Research and Education Center, Lake Alfred, and Deepak Shrestha, University of Florida Gainesville, for collecting data on monitor substrate voltages provided in the Supp Information (online only). Financial support for this research was provided by Frito Lay Inc., USDADARS State Cooperative Potato Research Program, Texas Department of Agriculture, U. S. Department of AgricultureDNational Institute of Food and AgricultureDRisk Avoidance and Mitigation (USDADNIFADRAMP) (Project # 2009-51101-05892), U. S. Department of AgricultureDNa-tional Institute of Food and AgricultureDThe Specialty Crop Research Initiative (USDADNIFADSCRI) (Project # 200951181- 20176) and in-house USDADARS funds from both Parlier and Wapato locations. The use of trade, firm, or corporation names in this publication is for information and convenience of the reader. Such use does not constitute an of financial endorsement or approval by the U. S. Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer. NR 42 TC 11 Z9 12 U1 1 U2 15 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD MAY PY 2014 VL 107 IS 3 BP 650 EP 666 DI 10.1603/AN13178 PG 17 WC Entomology SC Entomology GA AG8IS UT WOS:000335662600013 ER PT J AU Maul, JE Buyer, JS Lehman, RM Culman, S Blackwood, CB Roberts, DP Zasada, IA Teasdale, JR AF Maul, Jude E. Buyer, Jeffrey S. Lehman, R. Michael Culman, Steve Blackwood, Christopher B. Roberts, Daniel P. Zasada, Inga A. Teasdale, John R. TI Microbial community structure and abundance in the rhizosphere and bulk soil of a tomato cropping system that includes cover crops SO APPLIED SOIL ECOLOGY LA English DT Article DE Rhizosphere; Agroecology; Microbial; Crop rotation; perMANOVA; TRFLP ID BACTERIAL COMMUNITIES; NITROGEN AVAILABILITY; HAIRY VETCH; DIVERSITY; DISEASE; POPULATIONS; SUPPRESSION; MANAGEMENT; FRACTIONS; TILLAGE AB Understanding microbial responses to crop rotation and legacy of cropping history can assist in determining how land use management impacts microbially mediated soil processes. In the literature, one finds mixed results when attempting to determine the major environmental and biological controls on soil microbial structure and functionality. The objectives of this research were to: (1) Qualitatively and quantitatively measure seasonal and antecedent soil management effects on the soil microbial community structure in the rhizosphere of a subsequent tomato crop (So lanum lycopersicum) and (2) Determine phylum scale differences between the rhizosphere and bulk soil microbial community as influenced by the antecedent hairy vetch (Vicia villosa), cereal rye (Secale cereale), or black plastic mulch treatments. In this report, we use terminal restriction fragment length polymorphisms in the 16s rDNA gene to characterize changes in microbial community structure in soil samples from a field replicated tomato production system experiment at USDA-ARS Beltsville Agricultural Research Center, Beltsville, MD, USA. We found season of the year had the strongest influence on the soil microbial community structure of some of the major microbial phyla. Although we monitored just a few of the major microbial phyla (four Eubacteria and Archaea), we found that the effects of the tomato plant on the structural composition of these phyla in the rhizosphere differed dependent on the antecedent cover crop. Increased understanding of how agricultural factors influence the soil microbial community structure under field conditions is critical information for farmers and land managers to make decisions when targeting soil ecosystem services that are microbially driven. Published by Elsevier BAT. C1 [Maul, Jude E.; Buyer, Jeffrey S.; Roberts, Daniel P.; Teasdale, John R.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 44240 USA. [Lehman, R. Michael] USDA ARS, North Cent Agr Res Lab, Beltsville, MD 44240 USA. [Culman, Steve] Ohio State Univ, Dept Crop Sci, Columbus, OH 44240 USA. [Blackwood, Christopher B.] Kent State Univ, Dept Biol Sci, Kent, OH 44240 USA. [Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Beltsville, MD 44240 USA. RP Maul, JE (reprint author), USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD 44240 USA. EM Jude.Maul@ars.usda.gov RI Blackwood, Christopher/B-3839-2010; OI Blackwood, Christopher/0000-0001-5764-6978; Buyer, Jeffrey/0000-0003-2098-0547; Lehman, Michael/0000-0002-3391-3178 NR 52 TC 8 Z9 8 U1 10 U2 104 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD MAY PY 2014 VL 77 BP 42 EP 50 DI 10.1016/j.apsoil.2014.01.002 PG 9 WC Soil Science SC Agriculture GA AG9HX UT WOS:000335731100006 ER PT J AU Summers, CF Park, S Dunn, AR Rong, X Everts, KL Meyer, SLF Rupprecht, SM Kleinhenz, MD Gardener, BM Smart, CD AF Summers, Carly F. Park, Sunjeong Dunn, Amara R. Rong, Xiaoqing Everts, Kathryne L. Meyer, Susan L. F. Rupprecht, Shannon M. Kleinhenz, Matthew D. Gardener, Brian McSpadden Smart, Christine D. TI Single season effects of mixed-species cover crops on tomato health (cultivar Celebrity) in multi-state field trials SO APPLIED SOIL ECOLOGY LA English DT Article DE Cover crops; Phytophthora capsici; Plant-pathogenic nematodes; Soil fertility; Cultural practices; Organic agriculture ID PLANT-PARASITIC NEMATODES; MANAGEMENT-PRACTICES; PHYTOPHTHORA BLIGHT; PROCESSING TOMATOES; CROPPING SYSTEM; GREEN MANURE; BELL PEPPER; HAIRY VETCH; SOIL; SUPPRESSION AB Cover crop use can help mitigate the deleterious effects of common cropping practices (e.g., tillage) and is, therefore, an important component of soil health maintenance. While known to be beneficial in the longterm, the short-term effects of cover crops, specifically mixed-species cover crops in organic systems are less clear. Cover crop effects on tomato productivity and disease severity were recorded over three field seasons (2010, 2011 and 2012) at sixteen field sites in three states, Maryland, New York and Ohio (MD, NY and OH), each with distinct soilborne disease pressure. Plots of five state-specific cover crop treatments were established the season prior to tomato production; the resulting plant residue was incorporated the following spring approximately four weeks before tomato planting. Total fruit yields along with earlyseason shoot height and fresh weight were used to compare treatment effects on productivity. Treatment disease severity ratings relied on natural inoculum. Interestingly, the effect of a single season of cover cropping on total yield was significant in no more than 25% of all site years. Similarly, cover crop effects on tomato disease levels were significant in 0-44% of the sixteen field sites. However, significant fieldspecific patterns were observed in every state across multiple years for some treatments. For example, in New York in 2010, tomato yields following all mixed cover crops were greater than the single rye cover crop in one field, but this pattern was reversed in the adjacent field. Thus, no general recommendation of a specific cover crop mixture can be made for near-term enhancement of tomato productivity or for reduction of disease. Therefore, growers should focus on location and operation-specific variables when choosing cover crops.(c) 2014 Elsevier B.V. All rights reserved. C1 [Summers, Carly F.; Dunn, Amara R.; Smart, Christine D.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA. [Park, Sunjeong; Rong, Xiaoqing; Gardener, Brian McSpadden] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Everts, Kathryne L.] Univ Maryland, Lower Eastern Shore Res & Educ Ctr, Dept Plant Sci & Landscape Architecture, Salisbury, MD 21801 USA. [Meyer, Susan L. F.; Rupprecht, Shannon M.] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr, Nematol Lab, Beltsville, MD 20705 USA. [Kleinhenz, Matthew D.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Smart, CD (reprint author), Cornell Univ, 630 West North St, Geneva, NY 14456 USA. EM cds14@cornell.edu FU USDA's Organic Research and Extension Initiative (NIFA) [2009-51300-05512]; NSF Graduate Research Fellowship [DGE1144153] FX Thanks are extended to Holly Lange, Alexandra Curtis, Augusta Williams, Emily Wyckoff and Marci Day (Cornell University); Raymond Moore, Daniel Mongeon, Prasanti Babu, Ashlee Green and Wendy Mao (USDA ARS Nematology Laboratory); Matthew Hochmuth, Mason Newark, David Armentrout and Bernadette Gross (University of Maryland); and to Fulya Baysal, Martina Fraga, Seth Mauer, Elizabeth Kalikasingh, Miriam Gochnauer, Emily Nguyen, Jun Kyung Park, Seung Hawn Li, Sonia Walker, Jennifer Moyseenko and Michelle Young (The Ohio State University) for assistance in the laboratory, greenhouse and field. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Funding for this work was provided through the USDA's Organic Research and Extension Initiative (NIFA contract no. 2009-51300-05512). Funding for C.F. Summers was also provided through an NSF Graduate Research Fellowship (DGE1144153). NR 41 TC 3 Z9 3 U1 8 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD MAY PY 2014 VL 77 BP 51 EP 58 DI 10.1016/j.apsoil.2014.01.010 PG 8 WC Soil Science SC Agriculture GA AG9HX UT WOS:000335731100007 ER PT J AU Xiong, DG Wang, YL Ma, J Klosterman, SJ Xiao, SX Tian, CM AF Xiong, Dianguang Wang, Yonglin Ma, Jie Klosterman, Steven J. Xiao, Shuxiao Tian, Chengming TI Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae SO BMC GENOMICS LA English DT Article DE Verticillium dahliae; Microsclerotia development; RNA-Seq; Transcriptome; Gene expression; Alternative splicing ID SUPPRESSION SUBTRACTIVE HYBRIDIZATION; PROGRAMMED CELL-DEATH; GENE-EXPRESSION; FILAMENTOUS FUNGI; VEGETATIVE INCOMPATIBILITY; MELANIN BIOSYNTHESIS; HYDROPHOBIN GENE; FINE STRUCTURE; SEQ; ANNOTATION AB Background: Verticillium dahliae is a soil-borne fungus that causes vascular wilt diseases in a wide range of plant hosts. V. dahliae produces multicelled, melanized resting bodies, also known as microsclerotia (MS) that can survive for years in the soil. The MS are the primary source of infection of the Verticillium disease cycle. Thus, MS formation marks an important event in the disease cycle of V. dahliae. Results: In this study, next generation sequencing technology of RNA-Seq was employed to investigate the global transcriptomic dynamics of MS development to identify differential gene expression at several stages of MS formation in strain XS11 of V. dahliae, isolated from smoke tree. We observed large-scale changes in gene expression during MS formation, such as increased expression of genes involved in protein metabolism and carbohydrate metabolism. Genes involved in glycolytic pathway and melanin biosynthesis were dramatically up-regulated in MS. Cluster analyses revealed increased expression of genes encoding products involved in primary metabolism and stress responses throughout MS development. Differential expression of ubiquitin-dependent protein catabolism and cell death-associated genes during MS development were revealed. Homologs of genes located in the lineage-specific (LS) regions of V. dahliae strain VdLs. 17, were either not expressed or showed low expression. Furthermore, alternative splicing (AS) events were analyzed, revealing that over 95.0% AS events involve retention of introns (RI). Conclusions: These data reveal the dynamics of transcriptional regulation during MS formation and were used to construct a comprehensive high-resolution gene expression map. This map provides a key resource for understanding the biology and molecular basis of MS development of V. dahliae. C1 [Xiong, Dianguang; Wang, Yonglin; Xiao, Shuxiao; Tian, Chengming] Beijing Forestry Univ, Coll Forestry, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China. [Ma, Jie] Beijing Forestry Univ, Sch Informat Sci & Technol, Beijing, Peoples R China. [Klosterman, Steven J.] USDA ARS, Salinas, CA USA. RP Tian, CM (reprint author), Beijing Forestry Univ, Coll Forestry, Key Lab Silviculture & Conservat, Minist Educ, Beijing, Peoples R China. EM chengmt@bjfu.edu.cn FU National Natural Science Foundation of China [31370013, 31000302]; Fundamental Research Funds for the Central Universities [TD2011-06, YX2013-10]; Research Fund for the Doctoral Program of Higher Education [20100014120018] FX We are grateful to the Center for Computational Biology, Beijing Forestry University for providing the Linux platform. The research was supported by National Natural Science Foundation of China (31370013 and 31000302), the Fundamental Research Funds for the Central Universities (NO. TD2011-06 and YX2013-10), and the Research Fund for the Doctoral Program of Higher Education (20100014120018) to YW. NR 80 TC 10 Z9 11 U1 4 U2 52 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAY 1 PY 2014 VL 15 AR 324 DI 10.1186/1471-2164-15-324 PG 19 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AG8RK UT WOS:000335685700001 PM 24884698 ER PT J AU Freifeld, CC Brownstein, JS Menone, CM Bao, W Filice, R Kass-Hout, T Dasgupta, N AF Freifeld, Clark C. Brownstein, John S. Menone, Christopher M. Bao, Wenjie Filice, Ross Kass-Hout, Taha Dasgupta, Nabarun TI Digital Drug Safety Surveillance: Monitoring Pharmaceutical Products in Twitter SO DRUG SAFETY LA English DT Article ID REPORTING SYSTEM; SOCIAL MEDIA; ADVERSE; EVENTS AB Background Traditional adverse event (AE) reporting systems have been slow in adapting to online AE reporting from patients, relying instead on gatekeepers, such as clinicians and drug safety groups, to verify each potential event. In the meantime, increasing numbers of patients have turned to social media to share their experiences with drugs, medical devices, and vaccines. Objective The aim of the study was to evaluate the level of concordance between Twitter posts mentioning AE-like reactions and spontaneous reports received by a regulatory agency. Methods We collected public English-language Twitter posts mentioning 23 medical products from 1 November 2012 through 31 May 2013. Data were filtered using a semi-automated process to identify posts with resemblance to AEs (Proto-AEs). A dictionary was developed to translate Internet vernacular to a standardized regulatory ontology for analysis (MedDRA(A (R))). Aggregated frequency of identified product-event pairs was then compared with data from the public FDA Adverse Event Reporting System (FAERS) by System Organ Class (SOC). Results Of the 6.9 million Twitter posts collected, 4,401 Proto-AEs were identified out of 60,000 examined. Automated, dictionary-based symptom classification had 72 % recall and 86 % precision. Similar overall distribution profiles were observed, with Spearman rank correlation rho of 0.75 (p < 0.0001) between Proto-AEs reported in Twitter and FAERS by SOC. Conclusion Patients reporting AEs on Twitter showed a range of sophistication when describing their experience. Despite the public availability of these data, their appropriate role in pharmacovigilance has not been established. Additional work is needed to improve data acquisition and automation. C1 [Freifeld, Clark C.] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA. [Freifeld, Clark C.; Brownstein, John S.] Boston Childrens Hosp, Childrens Hosp, Informat Program, Boston, MA USA. [Brownstein, John S.] Harvard Univ, Sch Med, Dept Pediat, Boston, MA 02115 USA. [Brownstein, John S.] Harvard Univ, Sch Med, Ctr Biomed Informat, Boston, MA USA. [Menone, Christopher M.; Bao, Wenjie; Dasgupta, Nabarun] Epidem Inc, Boston, MA USA. [Filice, Ross] Georgetown Univ, Med Ctr, Washington, DC 20007 USA. [Kass-Hout, Taha] USDA, FDA, Silver Spring, MD USA. [Dasgupta, Nabarun] Univ N Carolina, Chapel Hill, NC 27599 USA. RP Dasgupta, N (reprint author), 266 Newbury St,2nd Floor, Boston, MA USA. EM nabarund@gmail.com OI Kass-Hout, Taha/0000-0002-0123-5157; Dasgupta, Nabarun/0000-0002-4098-605X FU FDA Office of the Chief Scientist FX The authors gratefully acknowledge our collaborators at the FDA, including Jesse Goodman, who was at the Office of the Chief Scientist at the time of writing, and Mark Walderhaug and Taxiarchis Botsis at the Center for Biologics Evaluation and Research, for their guidance and expertise. The work was funded by the FDA Office of the Chief Scientist; the funders had input into the study design. We thank two anonymous peer reviewers for helpful comments on an earlier version of the manuscript. NR 17 TC 51 Z9 53 U1 0 U2 12 PU ADIS INT LTD PI AUCKLAND PA 41 CENTORIAN DR, PRIVATE BAG 65901, MAIRANGI BAY, AUCKLAND 1311, NEW ZEALAND SN 0114-5916 EI 1179-1942 J9 DRUG SAFETY JI Drug Saf. PD MAY PY 2014 VL 37 IS 5 BP 343 EP 350 DI 10.1007/s40264-014-0155-x PG 8 WC Public, Environmental & Occupational Health; Pharmacology & Pharmacy; Toxicology SC Public, Environmental & Occupational Health; Pharmacology & Pharmacy; Toxicology GA AG9FX UT WOS:000335725900005 PM 24777653 ER PT J AU Pfeifer, M Lefebvre, V Gardner, TA Arroyo-Rodriguez, V Baeten, L Banks-Leite, C Barlow, J Betts, MG Brunet, J Cerezo, A Cisneros, LM Collard, S D'Cruze, N Motta, CD Duguay, S Eggermont, H Eigenbrod, F Hadley, AS Hanson, TR Hawes, JE Scalley, TH Klingbeil, BT Kolb, A Kormann, U Kumar, S Lachat, T Fraser, PL Lantschner, V Laurance, WF Leal, IR Lens, L Marsh, CJ Medina-Rangel, GF Melles, S Mezger, D Oldekop, JA Overal, WL Owen, C Peres, CA Phalan, B Pidgeon, AM Pilia, O Possingham, HP Possingham, ML Raheem, DC Ribeiro, DB Neto, JDR Robinson, WD Robinson, R Rytwinski, T Scherber, C Slade, EM Somarriba, E Stouffer, PC Struebig, MJ Tylianakis, JM Tscharntke, T Tyre, AJ Urbina-Cardona, JN Vasconcelos, HL Wearn, O Wells, K Willig, MR Wood, E Young, RP Bradley, AV Ewers, RM AF Pfeifer, Marion Lefebvre, Veronique Gardner, Toby A. Arroyo-Rodriguez, Victor Baeten, Lander Banks-Leite, Cristina Barlow, Jos Betts, Matthew G. Brunet, Joerg Cerezo, Alexis Cisneros, Laura M. Collard, Stuart D'Cruze, Neil da Silva Motta, Catarina Duguay, Stephanie Eggermont, Hilde Eigenbrod, Felix Hadley, Adam S. Hanson, Thor R. Hawes, Joseph E. Scalley, Tamara Heartsill Klingbeil, Brian T. Kolb, Annette Kormann, Urs Kumar, Sunil Lachat, Thibault Lakeman Fraser, Poppy Lantschner, Victoria Laurance, William F. Leal, Inara R. Lens, Luc Marsh, Charles J. Medina-Rangel, Guido F. Melles, Stephanie Mezger, Dirk Oldekop, Johan A. Overal, William L. Owen, Charlotte Peres, Carlos A. Phalan, Ben Pidgeon, Anna M. Pilia, Oriana Possingham, Hugh P. Possingham, Max L. Raheem, Dinarzarde C. Ribeiro, Danilo B. Ribeiro Neto, Jose D. Robinson, W. Douglas Robinson, Richard Rytwinski, Trina Scherber, Christoph Slade, Eleanor M. Somarriba, Eduardo Stouffer, Philip C. Struebig, Matthew J. Tylianakis, Jason M. Tscharntke, Teja Tyre, Andrew J. Urbina-Cardona, J. Nicolas Vasconcelos, Heraldo L. Wearn, Oliver Wells, Konstans Willig, Michael R. Wood, Eric Young, Richard P. Bradley, Andrew V. Ewers, Robert M. TI BIOFRAG - a new database for analyzing BIOdiversity responses to forest FRAGmentation SO ECOLOGY AND EVOLUTION LA English DT Article DE Bioinformatics; data sharing; database; edge effects; forest fragmentation; global change; landscape metrics; matrix contrast; species turnover ID HABITAT FRAGMENTATION; LANDSCAPE STRUCTURE; BREEDING BIRDS; EDGE; MATRIX; CONSERVATION; METAANALYSIS; COVER; SIZE; AREA AB Habitat fragmentation studies have produced complex results that are challenging to synthesize. Inconsistencies among studies may result from variation in the choice of landscape metrics and response variables, which is often compounded by a lack of key statistical or methodological information. Collating primary datasets on biodiversity responses to fragmentation in a consistent and flexible database permits simple data retrieval for subsequent analyses. We present a relational database that links such field data to taxonomic nomenclature, spatial and temporal plot attributes, and environmental characteristics. Field assessments include measurements of the response(s) (e.g., presence, abundance, ground cover) of one or more species linked to plots in fragments within a partially forested landscape. The database currently holds 9830 unique species recorded in plots of 58 unique landscapes in six of eight realms: mammals 315, birds 1286, herptiles 460, insects 4521, spiders 204, other arthropods 85, gastropods 70, annelids 8, platyhelminthes 4, Onychophora 2, vascular plants 2112, nonvascular plants and lichens 320, and fungi 449. Three landscapes were sampled as long-term time series (>10years). Seven hundred and eleven species are found in two or more landscapes. Consolidating the substantial amount of primary data available on biodiversity responses to fragmentation in the context of land-use change and natural disturbances is an essential part of understanding the effects of increasing anthropogenic pressures on land. The consistent format of this database facilitates testing of generalizations concerning biologic responses to fragmentation across diverse systems and taxa. It also allows the re-examination of existing datasets with alternative landscape metrics and robust statistical methods, for example, helping to address pseudo-replication problems. The database can thus help researchers in producing broad syntheses of the effects of land use. The database is dynamic and inclusive, and contributions from individual and large-scale data-collection efforts are welcome. C1 [Pfeifer, Marion; Lefebvre, Veronique; Banks-Leite, Cristina; Owen, Charlotte; Pilia, Oriana; Possingham, Hugh P.; Tylianakis, Jason M.; Wearn, Oliver; Bradley, Andrew V.; Ewers, Robert M.] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, Ascot SL5 7PY, Berks, England. [Gardner, Toby A.] Stockholm Environm Inst, Stockholm, Sweden. [Arroyo-Rodriguez, Victor] Univ Nacl Autonoma Mexico, Ctr Invest Ecosistemas, Morelia, Michoacan, Mexico. [Baeten, Lander] Univ Ghent, Dept Forest & Water Management, B-9000 Ghent, Belgium. [Barlow, Jos] Univ Lancaster, Lancaster Environm Ctr, Lancaster, England. [Betts, Matthew G.; Hadley, Adam S.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Brunet, Joerg] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, Alnarp, Sweden. [Cerezo, Alexis] Univ Buenos Aires, Dept Metodos Cuantitat & Sistemas Informac, Buenos Aires, DF, Argentina. [Cisneros, Laura M.; Klingbeil, Brian T.; Willig, Michael R.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT USA. [Cisneros, Laura M.; Klingbeil, Brian T.; Willig, Michael R.] Univ Connecticut, Ctr Environm Sci & Engn, Storrs, CT USA. [Collard, Stuart] Nat Conservat Soc South Australia, Adelaide, SA, Australia. [D'Cruze, Neil] World Soc Protect Anim, London, England. [da Silva Motta, Catarina] INPA, Dept Entomol, Manaus, Amazonas, Brazil. [Duguay, Stephanie] Carleton Univ, Geomat & Landscape Ecol Res Lab, Ottawa, ON K1S 5B6, Canada. [Eggermont, Hilde; Lens, Luc] Univ Ghent, Terr Ecol Unit, B-9000 Ghent, Belgium. [Eigenbrod, Felix; Phalan, Ben] Univ Southampton, Ctr Biol Sci, Southampton, Hants, England. [Hawes, Joseph E.; Peres, Carlos A.] Univ E Anglia, Sch Environm Sci, Norwich NR4 7TJ, Norfolk, England. [Scalley, Tamara Heartsill] USDA Forestry Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Kolb, Annette] Univ Bremen, Inst Ecol, FB2, D-28359 Bremen, Germany. [Kormann, Urs; Scherber, Christoph; Tscharntke, Teja] Univ Gottingen, Dept Crop Sci, D-37073 Gottingen, Germany. [Kumar, Sunil] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Lachat, Thibault] Swiss Fed Inst Forest Snow & Landscape Res WSL, Birmensdorf, Switzerland. [Lakeman Fraser, Poppy] Univ London Imperial Coll Sci Technol & Med, OPAL, London, England. [Lantschner, Victoria] Bariloche CONICET, INTA EEA, San Carlos De Bariloche, Rio Negro, Argentina. [Laurance, William F.] James Cook Univ, Ctr Trop Environm & Sustainabil Sci, Cairns, Qld, Australia. [Laurance, William F.] James Cook Univ, Sch Marine & Trop Biol, Cairns, Qld, Australia. [Leal, Inara R.; Ribeiro Neto, Jose D.] Univ Fed Pernambuco, Dept Bot, Recife, PE, Brazil. [Marsh, Charles J.] Univ Leeds, Fac Biol Sci, Leeds, W Yorkshire, England. [Medina-Rangel, Guido F.] Univ Nacl Colombia, ICN, Bogota, Colombia. [Melles, Stephanie] Univ Toronto, Dept Ecol & Evolutionary Biol, Toronto, ON, Canada. [Mezger, Dirk] Field Museum Nat Hist, Dept Zool, Chicago, IL 60605 USA. [Oldekop, Johan A.] Univ Sheffield, Sheffield Inst Int Dev, Sheffield, S Yorkshire, England. [Overal, William L.] Museu Paraense Emilio Goeldi, Dept Entomol, Belem, Para, Brazil. [Pidgeon, Anna M.; Wood, Eric] Univ Wisconsin Madison, Dept Forest & Wildlife Ecol, Madison, WI USA. [Possingham, Hugh P.] Univ Queensland, Brisbane, Qld, Australia. [Raheem, Dinarzarde C.] Royal Belgian Inst Nat Sci, Brussels, Belgium. [Raheem, Dinarzarde C.] Nat Hist Museum, Dept Life Sci, London SW7 5BD, England. [Ribeiro, Danilo B.] Univ Fed Mato Grosso do Sul, Ctr Ciencias Biol & Saude, Campo Grande, Brazil. [Robinson, W. Douglas] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR USA. [Robinson, Richard] Manjimup Res Ctr, Dept Pk & Wildlife, Manjimup, WA, Australia. [Rytwinski, Trina] Carleton Univ, Dept Biol, Ottawa, ON K1S 5B6, Canada. [Slade, Eleanor M.] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. [Somarriba, Eduardo] CATIE, Turrialba, Costa Rica. [Stouffer, Philip C.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Stouffer, Philip C.] INPA, BDFFP, Manaus, Amazonas, Brazil. [Struebig, Matthew J.] Univ Kent, Sch Anthropol & Conservat, Durrell Inst Conservat & Ecol, Canterbury, Kent, England. [Tylianakis, Jason M.] Univ Canterbury, Sch Biol Sci, Canterbury, New Zealand. [Tyre, Andrew J.] Univ Nebraska, Sch Nat Resources, Lincoln, NE USA. [Urbina-Cardona, J. Nicolas] Pontificia Univ Javeriana, Sch Rural & Environm Studies, Ecol & Terr Dept, Bogota, Colombia. [Vasconcelos, Heraldo L.] Univ Fed Uberlandia, Inst Biol, Uberlandia, MG, Brazil. [Wearn, Oliver] Zool Soc London, Inst Zool, London NW1 4RY, England. [Wells, Konstans] Univ Adelaide, Sch Earth & Environm Sci, Inst Environm, Adelaide, SA, Australia. [Young, Richard P.] Durrell Wildlife Conservat Trust, Trinity, Jersey, England. RP Pfeifer, M (reprint author), Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, Silwood Pk Campus, Ascot SL5 7PY, Berks, England. EM m.pfeifer@imperial.ac.uk RI Hadley, Adam/G-8391-2012; Kumar, Sunil/A-6730-2009; Arroyo-Rodriguez, Victor/C-9255-2009; Banks-Leite, Cristina/D-3075-2011; Peres, Carlos/B-1276-2013; Possingham, Hugh/B-1337-2008; Barlow, Jos/E-7861-2014; Urbina-Cardona, J. Nicolas/B-5447-2008; Marsh, Charles/D-2891-2012; Phalan, Benjamin/A-5783-2009; Wells, Konstans/A-7232-2010; Leal, Inara/E-8362-2010; Tylianakis, Jason/B-6634-2011; Ribeiro, Danilo/E-3104-2012; James Cook University, TESS/B-8171-2012; Tscharntke, Teja/N-5123-2014; Slade, Eleanor/O-3874-2014; Research ID, CTBCC /O-3564-2014 OI Pfeifer, Marion/0000-0002-6775-3141; Oldekop, Johan/0000-0003-0565-812X; Bradley, Andrew/0000-0002-6702-9751; Hadley, Adam/0000-0002-8344-3354; Banks-Leite, Cristina/0000-0002-0091-2857; Peres, Carlos/0000-0002-1588-8765; Possingham, Hugh/0000-0001-7755-996X; Wearn, Oliver/0000-0001-8258-3534; Ribeiro Neto, Jose Domingos/0000-0003-0505-7287; Scherber, Christoph/0000-0001-7924-8911; Urbina-Cardona, J. Nicolas/0000-0002-4174-8467; Marsh, Charles/0000-0002-0281-3115; Phalan, Benjamin/0000-0001-7876-7226; Wells, Konstans/0000-0003-0377-2463; Tylianakis, Jason/0000-0001-7402-5620; Ribeiro, Danilo/0000-0002-5343-9979; Slade, Eleanor/0000-0002-6108-1196; FU European Research Council (ERC) [281986] FX This paper is a contribution to Imperial College's Grand Challenges in Ecosystems and the Environment initiative. The research is funded by the European Research Council under the 7th Framework Program (FP7 ERC no. 281986). NR 95 TC 6 Z9 7 U1 9 U2 93 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD MAY PY 2014 VL 4 IS 9 BP 1524 EP 1537 DI 10.1002/ece3.1036 PG 14 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA AG2SQ UT WOS:000335267000003 PM 24967073 ER PT J AU Duquette, E Higgins, N Horowitz, J AF Duquette, Eric Higgins, Nathaniel Horowitz, John TI Inferring discount rates from time-preference experiments SO ECONOMICS LETTERS LA English DT Article DE Time preference; Discount rates; Experimental economics; Risk preference ID RISK AB We observe that identification of the discount rate from experimental data requires an assumption about the consumption period, the length of time over which a payment will be turned into utility-providing consumption. We show that the optimal consumption period is substantially longer than assumed in previous studies. When the consumption period is allowed to take on more reasonable values, the discount rates implied by experimental choices are unreasonably large and relatively insensitive to assumptions about utility curvature. Published by Elsevier B.V. C1 [Duquette, Eric; Higgins, Nathaniel] USDA, Econ Res Serv, Washington, DC 20174 USA. [Horowitz, John] US Dept Treasury, Off Tax Policy, Washington, DC 20220 USA. RP Higgins, N (reprint author), USDA, Econ Res Serv, 355 E St SW, Washington, DC 20174 USA. EM eduquette@ers.usda.gov; nathanielhiggins@live.com NR 14 TC 2 Z9 2 U1 0 U2 7 PU ELSEVIER SCIENCE SA PI LAUSANNE PA PO BOX 564, 1001 LAUSANNE, SWITZERLAND SN 0165-1765 EI 1873-7374 J9 ECON LETT JI Econ. Lett. PD MAY PY 2014 VL 123 IS 2 BP 212 EP 215 DI 10.1016/j.econlet.2014.02.009 PG 4 WC Economics SC Business & Economics GA AG7TZ UT WOS:000335623000024 ER PT J AU Penick, CA Brent, CS Dolezal, K Liebig, J AF Penick, Clint A. Brent, Colin S. Dolezal, Kelly Liebig, Juergen TI Neurohormonal changes associated with ritualized combat and the formation of a reproductive hierarchy in the ant Harpegnathos saltator SO JOURNAL OF EXPERIMENTAL BIOLOGY LA English DT Article DE Biogenic amines; Dopamine; Dominance; Aggression; Social insects ID DIVISION-OF-LABOR; HONEY-BEE COLONIES; PRIMITIVELY EUSOCIAL WASP; JUVENILE-HORMONE TITERS; AGE-RELATED-CHANGES; BIOGENIC-AMINES; APIS-MELLIFERA; PONERINE ANT; AGGRESSIVE-BEHAVIOR; BOMBUS-TERRESTRIS AB Dominance rank in animal societies is correlated with changes in both reproductive physiology and behavior. In some social insects, dominance status is used to determine a reproductive division of labor, where a few colony members reproduce while most remain functionally sterile. Changes in reproduction and behavior in this context must be coordinated through crosstalk between the brain and the reproductive system. We investigated a role for biogenic amines in forming this connection in the ant Harpegnathos saltator. In this species, workers engage in an elaborate dominance tournament to establish a group of reproductive workers termed gamergates. We analyzed biogenic amine content in the brains of gamergates, inside-workers and foragers under stable colony conditions and found that gamergates had the highest levels of dopamine. Dopamine levels were also positively correlated with increased ovarian activity among gamergates. Next, we experimentally induced workers to compete in a reproductive tournament to determine how dopamine may be involved in the establishment of a new hierarchy. Dopamine levels rose in aggressive workers at the start of a tournament, while workers that were policed by their nestmates (a behavior that inhibits ovarian activity) showed a rapid decline in dopamine. In addition to dopamine, levels of serotonin and tyramine differed among castes, and these changes could contribute to differences in caste-specific behavioral patterns observed among non-reproductive workers. Overall, these results provide support that biogenic amines link changes in behavior and dominance with reproductive activity in H. saltator as well as drive differences in worker task performance. C1 [Penick, Clint A.; Dolezal, Kelly; Liebig, Juergen] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Penick, Clint A.] N Carolina State Univ, Dept Biol, Raleigh, NC 27695 USA. [Brent, Colin S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Penick, CA (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. EM capenick@ncsu.edu NR 66 TC 13 Z9 13 U1 6 U2 42 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0022-0949 EI 1477-9145 J9 J EXP BIOL JI J. Exp. Biol. PD MAY PY 2014 VL 217 IS 9 BP 1496 EP 1503 DI 10.1242/jeb.098301 PG 8 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AG7FK UT WOS:000335583500018 PM 24436385 ER PT J AU Jandricic, SE Filotas, M Sanderson, JP Wraight, SP AF Jandricic, S. E. Filotas, M. Sanderson, J. P. Wraight, S. P. TI Pathogenicity of conidia-based preparations of entomopathogenic fungi against the greenhouse pest aphids Myzus persicae, Aphis gossypii, and Aulacorthum solani (Hemiptera: Aphididae) SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Mycoinsecticides; Beauveria; Metarhizium; Isaria; Virulence; Bioassays ID BEAUVERIA-BASSIANA; PAECILOMYCES-FUMOSOROSEUS; BEMISIA-ARGENTIFOLII; HOMOPTERA-APHIDIDAE; HYPOCREALES CORDYCIPITACEAE; MACROSIPHONIELLA-SANBORNI; LECANICILLIUM-ATTENUATUM; STANDARDIZED BIOASSAY; VERTICILLIUM-LECANII; BIOLOGICAL-CONTROL AB Seeking new isolates of entomopathogenic fungi with greater virulence against greenhouse aphid pests than those currently registered in North America for control of these insects, single-dose screening assays of 44 selected fungal isolates and 4 commercially available strains were conducted against first-instar nymphs of Myzus persicae and Aphis gossypii. The assays identified a number of Beauveria and Metarhizium isolates with virulence equal to or greater than that of the commercial strains against the nymphal aphids, but none exhibited exceptionally high virulence. Virulence of Isaria isolates was unexpectedly low (<31% mortality at doses >1000 conidia/mm(2)). In dose-response assays, Beauveria ARSEF 5493 proved most virulent against M. persicae and A. gossypii; however, LC(50)s of this isolate did not differ significantly from those of B. bassiana commercial strain JW-1. Dose-response assays were also conducted with Aulacorthum solani, the first reported evaluations of Beauveria and Metarhizium against this pest. The novel isolate Metarhizium 5471 showed virulence >= that of Beauveria 5493 in terms of LC25 and LC50, but 5493 produced a steeper dose response (slope). Additional tests showed that adult aphids are more susceptible than nymphs to fungal infection but confirmed that infection has a limited pre-mortem effect on aphid reproduction. Effects of assay techniques and the potential of fungal pathogens as aphid-control agents are discussed. (C) 2014 Elsevier Inc. All rights reserved. C1 [Jandricic, S. E.; Sanderson, J. P.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Filotas, M.] OMAFRA, Simcoe Off, Simcoe, ON N3Y 4N5, Canada. [Wraight, S. P.] Cornell Univ, USDA ARS, Robert W Holly Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Jandricic, S. E.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. RP Jandricic, SE (reprint author), N Carolina State Univ, Dept Entomol, Gardner Hall, Raleigh, NC 27695 USA. EM sej48@cornell.edu FU USDA-ARS; Cornell University (SCAs) [58-1907-9-033, 58-1907-4-447] FX Thank you to Mark Ramos for technical help. This research was fund, in part, through Specific Cooperative Agreements between USDA-ARS and Cornell University (SCAs #58-1907-9-033 and #58-1907-4-447). This paper reports results of research only. Mention of a proprietary product does not constitute an endorsement or recommendation by the USDA. NR 61 TC 7 Z9 9 U1 6 U2 52 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2014 VL 118 BP 34 EP 46 DI 10.1016/j.jip.2014.02.003 PG 13 WC Zoology SC Zoology GA AG6PN UT WOS:000335541000006 PM 24583227 ER PT J AU Chaudhury, MF Zhu, JJ Sagel, A Chen, H Skoda, SR AF Chaudhury, M. F. Zhu, J. J. Sagel, A. Chen, H. Skoda, S. R. TI Volatiles From Waste Larval Rearing Media Attract Gravid Screwworm Flies (Diptera: Calliphoridae) to Oviposit SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Cochliomyia hominivorax; Cochliomyia macellaria; electroantennogram; oviposition ID DIET AB Gravid screwworm flies, Cochliomyia hominivorax (Coquerel), are attracted to the volatiles from waste larval rearing media to deposit eggs. Studies were conducted to identify volatile chemicals from the waste larval media and determine their effectiveness to attract gravid flies to oviposit. Volatiles were collected using solid-phase microextraction method, and five active chemicals, namely, dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole, were identified using gas chromatography-mass spectrometry. In electroantennography studies, antennae of gravid screwworm flies, Cochliomyia macellaria (F.), responded positively to each of the identified compounds. A synthetic blend of these five compounds in the ratio of 335:200:57:1:12 was prepared and tested for its effectiveness to attract both C. hominivorax and C. macellaria using laboratory bioassay methods. Significantly more gravid C. macellaria were attracted to and landed on substrates treated with 10-fold diluted blends compared with those landed on substrates treated with ethanol only (as control). Only a few young females and young and old males were attracted to the substrates treated with the synthetic blend. The C. hominivorax females laid significantly more eggs on substrates treated with waste media, 10-fold diluted blend, and 100-fold diluted blend than on substrates with undiluted blend or ethanol. Similarly, C. macellaria deposited significantly more eggs on substrates treated with waste media, 10-fold diluted blend, and 100-fold diluted blend compared with substrates with undiluted blend or ethanol. C. macellaria females deposited significantly less amount of eggs than did C. hominivorax females. These results indicate that the synthetic blend of five compounds identified may serve as an oviposition attractant for C. hominivorax as well as for C. macellaria. C1 [Chaudhury, M. F.; Chen, H.] USDA ARS, Screwworm Res Unit, Lincoln, NE 68583 USA. [Chaudhury, M. F.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Sagel, A.] USDA ARS, COPEG, Screwworm Res Unit, Pacora, Panama. [Skoda, S. R.] USDA ARS, Screwworm Res Unit, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Chaudhury, MF (reprint author), USDA ARS, Screwworm Res Unit, Entomology Hall,UNL EC, Lincoln, NE 68583 USA. EM Muhammad.Chaudhury@ars.usda.gov NR 9 TC 5 Z9 5 U1 0 U2 10 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2014 VL 51 IS 3 BP 591 EP 595 DI 10.1603/ME13193 PG 5 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA AG8IB UT WOS:000335660900012 PM 24897851 ER PT J AU Reid, WR Thornton, A Pridgeon, JW Becnel, JJ Tang, F Estep, A Clark, GG Allan, S Liu, NN AF Reid, William R. Thornton, Anne Pridgeon, Julia W. Becnel, James J. Tang, Fang Estep, Alden Clark, Gary G. Allan, Sandra Liu, Nannan TI Transcriptional Analysis of Four Family 4 P450s in a Puerto Rico Strain of Aedes aegypti (Diptera: Culicidae) Compared With an Orlando Strain and Their Possible Functional Roles in Permethrin Resistance SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Aedes aegytpi; permethrin; resistance; cytochrome P450; detoxification ID INSECTICIDE RESISTANCE; MOLECULAR-BASIS; DENGUE VECTOR; DETOXIFICATION GENES; IMPREGNATED BEDNETS; ANOPHELES-GAMBIAE; METABOLISM; EXPRESSION; DROSOPHILA; MOSQUITOS AB A field strain of Aedes aegypti (L.) was collected from Puerto Rico in October 2008. Based on LD50 values by topical application, the Puerto Rico strain was 73-fold resistant to permethrin compared with a susceptible Orlando strain. In the presence of piperonyl butoxide, the resistance of Puerto Rico strain of Ae. aegypti was reduced to 15-fold, suggesting that cytochrome P450-mediated detoxification is involved in the resistance of the Puerto Rico strain to permethrin. To determine the cytochrome P450s that might play a role in the resistance to permethrin, the transcriptional levels of 164 cytochrome P450 genes in the Puerto Rico strain were compared with that in the Orlando strain. Of the 164 cytochrome P450s, 33 were significantly (P < 0.05) up-regulated, including cytochrome P450s in families four, six, and nine. Multiple studies have investigated the functionality of family six and nine cytochrome P450s, therefore, we focused on the up-regulated family 4 cytochrome P450s. To determine whether up-regulation of the four cytochrome P450s had any functional role in permethrin resistance, transgenic Drosophila melanogaster Meigen lines overexpressing the four family 4 P450 genes were generated, and their ability to survive exposure to permethrin was evaluated. When exposed to 5 mu g per vial permethrin, transgenic D. melanogaster expressing CYP4D24, CYP4H29, CYP4J15v1, and CYP4H33 had a survival rate of 60.0 +/- 6.7, 29.0 +/- 4.4, 64.4 +/- 9.7, and 11.0 +/- 4.4%, respectively. However, none of the control flies survived the permethrin exposure at the same concentration. Similarly, none of the transgenic D. melanogaster expressing CYP4J15v1 or CYP4H33 ? 5 survived when they were exposed to permethrin at 10 mu g per vial. However, transgenic D. melanogaster expressing CYP4D24 and CYP4H29 had a survival rate of 37.8 +/- 4.4 and 2.2 +/- 2.2%, respectively. Taken together, our results suggest that CYP4D24 might play an important role in cytochrome P450-mediated resistance to permethrin. C1 [Reid, William R.; Tang, Fang; Liu, Nannan] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Thornton, Anne; Estep, Alden] Navy Entomol Ctr Excellence, Jacksonville, FL 32312 USA. [Pridgeon, Julia W.; Becnel, James J.; Estep, Alden; Clark, Gary G.; Allan, Sandra] USDA ARS, Ctr Med Agr & Vet Entomol, Mosquito & Fly Res Unit, Gainesville, FL 32682 USA. RP Pridgeon, JW (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM Julia.pridgeon@ars.usda.gov FU Deployed War-Fighter Protection program FX We thank Neil Sanscrainte, Matthew Brown, Francis Golden, and Nathan Newlon for their assistance with the rearing of the mosquitoes. We also thank the Deployed War-Fighter Protection program for funding. NR 41 TC 9 Z9 9 U1 0 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2014 VL 51 IS 3 BP 605 EP 615 DI 10.1603/ME13228 PG 11 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA AG8IB UT WOS:000335660900014 PM 24897853 ER PT J AU Carroll, JF Kramer, M Bedoukian, RH AF Carroll, J. F. Kramer, M. Bedoukian, R. H. TI Solvent, Drying Time, and Substrate Affect the Responses of Lone Star Ticks (Acari: Ixodidae) to the Repellents Deet and Picaridin SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Amblyomma americanum; acetone; ethanol; water ID AMBLYOMMA-AMERICANUM ACARI; IXODES-SCAPULARIS; IN-VITRO; MOSQUITO REPELLENTS; CARBON-DIOXIDE; UNITED-STATES; ESSENTIAL OIL; LYME-DISEASE; N,N-DIETHYL-M-TOLUAMIDE; EVAPORATION AB Behavioral bioassays remain a standard tool in the discovery, development, and registration of arthropod repellents. Tick repellent bioassays are generally uncomplicated, but their results can be affected by basic variables (e.g., dimensions of testing materials, substrate, timing, temperature) of the assay. Using lone star tick, Amblyomma americanum (L.), nymphs in climbing bioassays, we tested for the effects of substrate, solvent, and drying time on tick responses. In dose-response tests, the widely used repellents N,N-diethyl-3-methyl benzamide (deet) and 1-methylpropyl-2-(hydroxyethyl)-1-piperidinecarboxylate (picaridin) were applied to filter paper strips and challenged by ticks at 10, 20, 30, 40, and 120 min after application. At 10-min drying time, repellency at the intermediate concentration 500 nmol repellent/cm(2) filter paper was significantly lower for ethanol solutions of deet and picaridin (0 and 10% ticks repelled, respectively) than for solutions of deet and picaridin in acetone (96.7 and 76.7% ticks repelled, respectively). Repellency was greatest for both the acetone and ethanol solutions of deet and picaridin when challenged 120 min after application, and at shorter drying times at the highest concentration tested (2,000 nmol compound/cm(2)). The repellency of picaridin relative to deet differed at some combinations of solvent and drying time but not others. In dose-response tests using different paper substrates and a drying time of 10 min, both ethanol and acetone solutions of deet differed in repellency, depending on both the paper substrate and the solvent. However, there were no differences in repellency between ethanol and acetone solutions of deet applied to nylon organdy in an in vitro and in an in vivo (fingertip) bioassay. When deet in solution with various proportions of ethanol: water was applied at 2,000 nmol deet/cm(2) filter paper, the proportion of ticks repelled decreased as the proportion of water in the test solutions increased. Somewhat similar results were seen for solutions of deet in an acetone solvent. Water absorbed from the atmosphere may affect the efficacy of repellents in solution with anhydrous ethanol. Overall, results obtained from bioassays that differ in seemingly minor ways can be surprisingly different, diminishing the value of comparing studies that used similar, but not identical, methods. Nylon organdy or another similar thin cloth may be preferable to filter papers and copier paper for minimizing solvent-related differences. When a paper substrate is used, acetone may be the more suitable solvent if the solubility of the test compound and other factors allow. C1 [Carroll, J. F.] USDA ARS, Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Kramer, M.] USDA ARS, Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Bedoukian, R. H.] Bedoukian Res Inc, Danbury, CT 06810 USA. RP Kramer, M (reprint author), USDA ARS, Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. EM matt.kramer@ars.usda.gov NR 38 TC 0 Z9 0 U1 3 U2 13 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2014 VL 51 IS 3 BP 629 EP 637 DI 10.1603/ME12214 PG 9 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA AG8IB UT WOS:000335660900016 PM 24897855 ER PT J AU Hughes, TH Richardson, AG Hoel, DF Mejeoumov, T Farooq, M Stoops, CA AF Hughes, Tony H. Richardson, Alec G. Hoel, David F. Mejeoumov, Tracy Farooq, Mohammad Stoops, Craig A. TI Suppression of Amblyomma americanum (Ixodida: Ixodidae) for Short-Term Field Operations Utilizing Cypermethrin and Lambda-Cyhalothrin SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE Amblyomma americanum; cypermethrin; lambda-cyhalothrin; military ID IXODES-SCAPULARIS; ACARI IXODIDAE; AREA CONTROL; TICK ACARI; NYMPHS; NOOTKATONE AB Tick-borne diseases pose risks to U. S. military personnel who conduct operations, both domestic and abroad. To determine the feasibility of protecting personnel from tick vectors during short-term field deployments, acaricides cypermethrin (Demon WP, Syngenta, Greensboro, NC) and lambda-cyhalothrin (Surrender Pestabs, CSI, Pasadena, TX) were applied to plots within two separate field sites on Camp Blanding Joint Training Center in Starke, FL, from May to June 2011. We analyzed their effectiveness in reducing tick counts for 6 wk after application. In total, 8,193 ticks were identified and counted, of which >99% were a mix of nymphs and adult-stage Amblyomma americanum (L.). Our results indicate that both cypermethrin and lambda-cyhalothrin were effective in significantly reducing tick numbers and preventing entry into treated plots for 6 wk after application. Thus, these two acaracides can be used to effectively suppress tick populations and provide residual protection in small geographic areas of recreation or public health significance. C1 [Hughes, Tony H.; Richardson, Alec G.; Mejeoumov, Tracy; Farooq, Mohammad] Navy Entomol Ctr Excellence, Operat Assessment Dept, Naval Air Stn Jacksonville, Jacksonville, FL 32212 USA. [Hoel, David F.] US Ctr Dis Control & Prevent, Navy & Marine Corps Publ Hlth Ctr Detachment CDC, Atlanta, GA 30329 USA. [Stoops, Craig A.] USDA ARS, Navy & Marine Corps Publ Hlth Ctr Detachment, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Hughes, TH (reprint author), Navy Entomol Ctr Excellence, Operat Assessment Dept, Naval Air Stn Jacksonville, Bldg 937, Jacksonville, FL 32212 USA. EM tony.hughes@med.navy.mil FU Deployed War fighter Protection Research Program (DWFP) FX We thank Rosa Ergas of the Navy and Marine Corps Public Health Center for providing tick-borne disease data to the Department of Defense. We gratefully acknowledge the staff of the Navy Entomology Center of Excellence for their assistance with the fieldwork. We also thank Carl Doud, Peter Obenauer, and Graham White for a thorough review of the manuscript. This work was supported by the Deployed War fighter Protection Research Program (DWFP). NR 17 TC 1 Z9 1 U1 0 U2 7 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2014 VL 51 IS 3 BP 709 EP 712 DI 10.1603/ME13034 PG 4 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA AG8IB UT WOS:000335660900027 PM 24897866 ER PT J AU Szewczyk, E Kasuga, T Fan, ZL AF Szewczyk, Edyta Kasuga, Takao Fan, Zhiliang TI A new variant of self-excising beta-recombinase/six cassette for repetitive gene deletion and homokaryon purification in Neurospora crassa SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Neurospora crassa; Repetitive gene deletion; Marker recycling; Thymidine kinase; Endoxylanase gh10-2 inducible promoter; Negative selection marker ID SITE-SPECIFIC RECOMBINATION; NITROGEN REGULATORY PROTEIN; ASPERGILLUS-FUMIGATUS; CANDIDA-ALBICANS; TRANSCRIPTION FACTOR; RECYCLABLE MARKER; FILAMENTOUS FUNGI; THYMIDINE KINASE; BINDING PROTEIN; DNA-BINDING AB In a previous study, we developed a cassette employing a bacterial p-recombinase acting on six recognition sequences (beta-rec/six), which allowed repetitive site-specific gene deletion and marker recycling in Neurospora crassa. However, only one positive selection marker was used in the cassette. A tedious subsequent procedure was needed to purify homokaryons due to the lack of a negative selection after cassette eviction. Additionally, the endoxylanase xylP promoter from Penicillium chrysogenum used in the construct was not strongly regulated in N. crassa, which led to low efficiency in cassette eviction. Herein we report an improved variant of the self-excising beta-recombinase/six cassette for repetitive gene deletions in N. crassa using a native endoxylanase gh10-2 promoter from N. crassa, plus the introduction of a bidirectional selection marker to facilitate homokaryon selection using a thymidine kinase (tic) gene (negative selection) in addition to the phosphinothricin resistance gene (bar(r)) (positive selection). (c) 2014 Elsevier B.V. All rights reserved. C1 [Szewczyk, Edyta; Fan, Zhiliang] Univ Calif Davis, Biol & Agr Engn Dept, Davis, CA 95616 USA. [Kasuga, Takao] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Kasuga, Takao] USDA ARS, Davis, CA 95616 USA. RP Fan, ZL (reprint author), Univ Calif Davis, Biol & Agr Engn Dept, One Shields Ave, Davis, CA 95616 USA. EM jzfan@ucdavis.edu FU Agriculture and Food Research Initiative Competitive from the USDA's National Institute of Food and Agriculture [2011-67009-20060] FX We thank Dr. Sven Krappmann (Microbiology Institute - Clinical Microbiology, Immunology and Hygiene University Hospital and Friedrich-Alexander-University of Erlangen-Nurnberg) for the generous gift of pSK397 and constructive discussions; Katherine Borkovich for the bar gene sequence and communications; and Matthew Sachs for the tk gene advice. This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2011-67009-20060 from the USDA's National Institute of Food and Agriculture. The authors thank Professor Rebecca Parales for the NanoDrop equipment. NR 78 TC 1 Z9 1 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD MAY PY 2014 VL 100 BP 17 EP 23 DI 10.1016/j.mimet.2014.02.007 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA AG7XD UT WOS:000335631200003 PM 24556286 ER PT J AU Guo, XY Jakes, JE Banna, S Nishi, Y Shohet, JL AF Guo, Xiangyu Jakes, Joseph E. Banna, Samer Nishi, Yoshio Shohet, J. Leon TI Effect of water uptake on the fracture behavior of low-k organosilicate glass SO JOURNAL OF VACUUM SCIENCE & TECHNOLOGY A LA English DT Article ID DIELECTRIC-CONSTANT MATERIALS; MECHANICAL-PROPERTIES; FILMS; NANOINDENTATION; INDENTATION; TOUGHNESS; ADHESION; PLASMA; MICROELECTRONICS; STRESS AB Water uptake in porous low-k dielectrics has become a significant challenge for both back-end-of-the- line integration and circuit reliability. This work examines the effects of water uptake on the fracture behavior of nanoporous low-k organosilicate glass. By using annealing dehydration and humidity conditioning, the roles of different water types and their concentrations are analyzed in detail. For as-deposited SiCOH films, annealing dehydration can enhance the resistance to crack occurrence, and these enhancements can be offset by higher humidity conditioning. It was found that the film-cracking threshold can be lowered by in-diffused water in the film as well as by water at the SiCOH/subtract interface. This occurs because the water decreases the film fracture energy and adhesion energy, respectively. By conditioning at high humidity, the variation of the film cracking threshold agrees well with the behavior of the film hardness and modulus of elasticity as a function of relative humidity. The crack morphologies of low-k porous films are also implicitly related to water uptake in the materials. Film cracking thresholds and crack morphologies of UV-cured low-k materials exhibit a weaker dependence on the water uptake, indicating a low degree of hydrophilicity of the SiCOH film after UV curing, which corroborates the previous results. Furthermore, by measuring the surface crack length, the material-fracture toughness can be found. The results demonstrate that neither annealing dehydration nor water uptake have significant effects on fracture toughness of as-deposited SiCOH, while for UV-cured SiCOH, annealing enhances the film-fracture toughness. (C) 2014 American Vacuum Society. C1 [Guo, Xiangyu; Shohet, J. Leon] Univ Wisconsin, Plasma Proc & Technol Lab, Madison, WI 53706 USA. [Guo, Xiangyu; Shohet, J. Leon] Univ Wisconsin, Dept Elect & Comp Engn, Madison, WI 53706 USA. [Jakes, Joseph E.] ARS, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Banna, Samer] Appl Mat Inc, Sunnyvale, CA 94085 USA. [Nishi, Yoshio] Stanford Univ, Dept Elect Engn, Stanford, CA 94305 USA. RP Guo, XY (reprint author), Univ Wisconsin, Plasma Proc & Technol Lab, Madison, WI 53706 USA. EM shohet@engr.wisc.edu FU Semiconductor Research Corporation [2012-KJ-2359]; National Science Foundation [CBET-1066231]; Applied Materials University Research Partnership Program; USDA Forest Service PECASE funding FX This work was supported by the Semiconductor Research Corporation under Contract No. 2012-KJ-2359, by the National Science Foundation under Grant CBET-1066231 and by the Applied Materials University Research Partnership Program. J. E. Jakes is supported by USDA Forest Service PECASE funding. NR 31 TC 5 Z9 5 U1 0 U2 6 PU A V S AMER INST PHYSICS PI MELVILLE PA STE 1 NO 1, 2 HUNTINGTON QUADRANGLE, MELVILLE, NY 11747-4502 USA SN 0734-2101 EI 1520-8559 J9 J VAC SCI TECHNOL A JI J. Vac. Sci. Technol. A PD MAY PY 2014 VL 32 IS 3 AR 031512 PG 8 WC Materials Science, Coatings & Films; Physics, Applied SC Materials Science; Physics GA AH2QF UT WOS:000335965300027 ER PT J AU Pantin-Jackwood, MJ Miller, PJ Spackman, E Swayne, DE Susta, L Costa-Hurtado, M Suarez, DL AF Pantin-Jackwood, Mary J. Miller, Patti J. Spackman, Erica Swayne, David E. Susta, Leonardo Costa-Hurtado, Mar Suarez, David L. TI Role of Poultry in the Spread of Novel H7N9 Influenza Virus in China SO JOURNAL OF VIROLOGY LA English DT Article ID AVIAN INFLUENZA-VIRUS-A/CHICKEN/INDONESIA/2003 H5N1; PIGEONS COLUMBIA-LIVIA; A VIRUS; HONG-KONG; HUMAN INFECTION; JAPANESE-QUAIL; RECEPTOR SPECIFICITY; WILD BIRDS; HOST-RANGE; CHICKENS AB The recent outbreak of H7N9 influenza in China has resulted in many human cases with a high fatality rate. Poultry are the likely source of infection for humans on the basis of sequence analysis and virus isolations from live bird markets, but it is not clear which species of birds are most likely to be infected and shedding levels of virus sufficient to infect humans. Intranasal inoculation of chickens, Japanese quail, pigeons, Pekin ducks, Mallard ducks, Muscovy ducks, and Embden geese with 106 50% egg infective doses of the A/Anhui/1/2013 virus resulted in infection but no clinical disease signs. Virus shedding was much higher and prolonged in quail and chickens than in the other species. Quail effectively transmitted the virus to direct contacts, but pigeons and Pekin ducks did not. In all species, virus was detected at much higher titers from oropharyngeal swabs than cloacal swabs. The hemagglutinin gene from samples collected from selected experimentally infected birds was sequenced, and three amino acid differences were commonly observed when the sequence was compared to the sequence of A/Anhui/1/2013: N123D, N149D, and L217Q. Leucine at position 217 is highly conserved for human isolates and is associated with alpha 2,6-sialic acid binding. Different amino acid combinations were observed, suggesting that the inoculum had viral subpopulations that were selected after passage in birds. These experimental studies corroborate the finding that certain poultry species are reservoirs of the H7N9 influenza virus and that the virus is highly tropic for the upper respiratory tract, so testing of bird species should preferentially be conducted with oropharyngeal swabs for the best sensitivity. C1 [Pantin-Jackwood, Mary J.; Miller, Patti J.; Spackman, Erica; Swayne, David E.; Susta, Leonardo; Costa-Hurtado, Mar; Suarez, David L.] Agr Res Serv, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, USDA, Athens, GA USA. RP Suarez, DL (reprint author), Agr Res Serv, Exot & Emerging Avian Viral Dis Res Unit, Southeast Poultry Res Lab, USDA, Athens, GA USA. EM david.suarez@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 FU U.S. Department of Agriculture CRIS [6612-32000-063-00D]; National Institute of Allergy; Infectious Diseases, National Institutes of Health (NIH) [AAI12004-001-00001] FX This research was supported by U.S. Department of Agriculture CRIS Project 6612-32000-063-00D and federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH), under IAA no. AAI12004-001-00001. NR 58 TC 46 Z9 50 U1 3 U2 23 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAY PY 2014 VL 88 IS 10 BP 5381 EP 5390 DI 10.1128/JVI.03689-13 PG 10 WC Virology SC Virology GA AG5GB UT WOS:000335446400018 PM 24574407 ER PT J AU Kliot, A Cilia, M Czosnek, H Ghanim, M AF Kliot, Adi Cilia, Michelle Czosnek, Henryk Ghanim, Murad TI Implication of the Bacterial Endosymbiont Rickettsia spp. in Interactions of the Whitefly Bemisia tabaci with Tomato Yellow Leaf Curl Virus SO JOURNAL OF VIROLOGY LA English DT Article ID ACCESSORY SALIVARY-GLANDS; APHID TRANSMISSION; DWARF VIRUS; TRANSOVARIAL TRANSMISSION; CIRCULATIVE TRANSMISSION; BEGOMOVIRUS TRANSMISSION; HEMIPTERA ALEYRODIDAE; VIRION STABILITY; 17-KDA PROTEIN; AEDES-AEGYPTI AB Numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. Tomato yellow leaf curl virus (TYLCV) is transmitted by the sweet potato whitefly Bemisia tabaci. We report here that infection with Rickettsia spp., a facultative endosymbiont of whiteflies, altered TYLCV-B. tabaci interactions. A B. tabaci strain infected with Rickettsia acquired more TYLCV from infected plants, retained the virus longer, and exhibited nearly double the transmission efficiency compared to an uninfected B. tabaci strain with the same genetic background. Temporal and spatial antagonistic relationships were discovered between Rickettsia and TYLCV within the whitefly. In different time course experiments, the levels of virus and Rickettsia within the insect were inversely correlated. Fluorescence in situ hybridization analysis of Rickettsia-infected midguts provided evidence for niche exclusion between Rickettsia and TYLCV. In particular, high levels of the bacterium in the midgut resulted in higher virus concentrations in the filter chamber, a favored site for virus translocation along the transmission pathway, whereas low levels of Rickettsia in the midgut resulted in an even distribution of the virus. Taken together, these results indicate that Rickettsia, by infecting the midgut, increases TYLCV transmission efficacy, adding further insights into the complex association between persistent plant viruses, their insect vectors, and microorganism tenants that reside within these insects. C1 [Kliot, Adi; Ghanim, Murad] Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel. [Kliot, Adi; Czosnek, Henryk] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel. [Cilia, Michelle] Cornell Univ, USDA ARS, Boyce Thompson Inst Plant Res, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Ghanim, M (reprint author), Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel. EM ghanim@agri.gov.il NR 43 TC 14 Z9 17 U1 3 U2 64 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAY PY 2014 VL 88 IS 10 BP 5652 EP 5660 DI 10.1128/JVI.00071-14 PG 9 WC Virology SC Virology GA AG5GB UT WOS:000335446400040 PM 24600010 ER PT J AU Torres, O Matute, J Gelineau-van Waes, J Maddox, JR Gregory, SG Ashley-Koch, AE Showker, JL Zitomer, NC Voss, KA Riley, RT AF Torres, Olga Matute, Jorge Gelineau-van Waes, Janee Maddox, Joyce R. Gregory, Simon G. Ashley-Koch, Allison E. Showker, Jency L. Zitomer, Nicholas C. Voss, Kenneth A. Riley, Ronald T. TI Urinary fumonisin B-1 and estimated fumonisin intake in women from high-and low-exposure communities in Guatemala SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Estimated fumonisin intake; Fumonisin; Fusarium verticillioides; Maize consumption; Urinary fumonisin B-1 ID NEURAL-TUBE DEFECTS; MAIZE; BIOMARKERS; TOXICITY; POPULATIONS; METABOLISM; HUMANS; MICE AB ScopeFumonisin (FB) intake can be high when maize is a dietary staple. We determined (i) urinary FB (UFB) in women consuming maize in high- and low-exposure communities in Guatemala, (ii) the FB levels in maize, (iii) the relationship between UFB and FB intake, and (iv) the relative excretion of UFB1, UFB2, and UFB3. Methods and resultsUrine and maize were analyzed for FB for 1 year in three departments. Maize consumption was estimated by an interview questionnaire. Fumonisin B-1, B-2, and B-3 (FB1, FB2 and FB3), were detected in 100% of maize samples. FB1 in maize and urine was significantly higher in Jutiapa compared to Chimaltenango or Escuintla. The FB intake paralleled UFB1 in a dose-dependent manner but UFB1 was present in much higher levels than UFB2 or UFB3 compared to maize. ConclusionIn Jutiapa, agroecological conditions favored FB production. UFB1 mirrored the estimated FB intake. UFB1 > 0.1 ng/mL resulted in a dose-dependent increase in the risk of exceeding FB intake of 2 g/kg b.w./day compared to women with no detectable UFB1. More than 50% exceeded 2 g/kg b.w./day when UFB1 was >0.5 ng/mL. UFB2 and UFB3 were rarely detected confirming that FB1 is either absorbed better or preferentially excreted in urine. C1 [Torres, Olga; Matute, Jorge] Ctr Invest Nutr & Salud, Guatemala City, Guatemala. [Gelineau-van Waes, Janee; Maddox, Joyce R.] Creighton Univ, Sch Med, Dept Pharmacol, Omaha, NE 68178 USA. [Gregory, Simon G.; Ashley-Koch, Allison E.] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA. [Showker, Jency L.; Zitomer, Nicholas C.; Voss, Kenneth A.; Riley, Ronald T.] USDA ARS, Toxicol & Mycotoxin Res Unit, RB Russell Res Ctr, Athens, GA 30605 USA. RP Riley, RT (reprint author), USDA ARS, Toxicol & Mycotoxin Res Unit, RB Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM ron.riley@ars.usda.gov OI Ashley-Koch, Allison/0000-0001-5409-9155 FU USDA-ARS from the Eunice Kennedy Shriver National Institute of Child Health & Human Development [NP108, 6612-42000-012-00D, RC4HD067971-01] FX The authors would like to thank all the women who participated in this study, the Ministry of Health of Guatemala, and the leaders in the communities of Chimaltenango, Escuintla, and Jutiapa, without whose cooperation this study could not have been performed. The authors also would like to thank Adela Ruiz, Rosa Chovix, and Waldemar Gonzalez for the field work and sample collection in Guatemala, Marta Maria Mendez, Cecilia de Mayorga, Luis Rodriguez, and Flor Diaz for the urine and maize extraction in Guatemala and Dr. Zaid Abdo (USDA-ARS South Atlantic Area) for assistance with and review of the statistical analysis. This work was supported by USDA-ARS NP108 in house project 6612-42000-012-00D and Award Number RC4HD067971-01 from the Eunice Kennedy Shriver National Institute of Child Health & Human Development. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Eunice Kennedy Shriver National Institute of Child Health & Human Development or the National Institutes of Health. NR 25 TC 11 Z9 11 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD MAY PY 2014 VL 58 IS 5 BP 973 EP 983 DI 10.1002/mnfr.201300481 PG 11 WC Food Science & Technology SC Food Science & Technology GA AF5SO UT WOS:000334773400006 PM 24375966 ER PT J AU Lee, BH Lin, AHM Nichols, BL Jones, K Rose, DR Quezada-Calvillo, R Hamaker, BR AF Lee, Byung-Hoo Lin, Amy Hui-Mei Nichols, Buford L. Jones, Kyra Rose, David R. Quezada-Calvillo, Roberto Hamaker, Bruce R. TI Mucosal C-terminal maltase-glucoamylase hydrolyzes large size starch digestion products that may contribute to rapid postprandial glucose generation SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE ctMGAM; Glucogenesis; Glycemic spike; Mucosal -glucosidases; Starch digestion ID INTESTINAL SUCRASE-ISOMALTASE; ALPHA-GLUCOSIDASE; GLYCEMIC INDEX; BLOOD-GLUCOSE; SPECIFICITY; INVERTASE; CLASSIFICATION; CHROMATOGRAPHY; INHIBITION; SUBUNIT AB ScopeThe four mucosal -glucosidases, which differ in their digestive roles, generate glucose from glycemic carbohydrates and accordingly can be viewed as a control point for rate of glucose delivery to the body. In this study, individual recombinant enzymes were used to understand how -glucan oligomers are digested by each enzyme, and how intermediate -amylolyzed starches are hydrolyzed, to elucidate a strategy for moderating the glycemic spike of rapidly digestible starchy foods. Methods and resultsThe C-terminal maltase-glucoamylase (ctMGAM, commonly termed glucoamylase) was able to rapidly hydrolyze longer maltooligosaccharides, such as maltotetraose and maltopentaose, to glucose. Moreover, it was found to convert larger size maltodextrins, as would be produced early in -amylase digestion of starch, efficiently to glucose. It is postulated that ctMGAM has the additional capacity to hydrolyze large -amylase products that are produced immediately on starch digestion in the duodenum and contribute to the rapid generation of glucose from starch-based meals. ConclusionThe findings suggest that partial inhibition of ctMGAM, such as by natural inhibitors found in foods, might be used to moderate the early stage of high glycemic response, as well as to extend digestion distally; thereby having relevance in regulating glucose delivery to the body. C1 [Lee, Byung-Hoo; Lin, Amy Hui-Mei; Hamaker, Bruce R.] Purdue Univ, Whistler Ctr Carbohydrate Res, W Lafayette, IN 47907 USA. [Lee, Byung-Hoo; Lin, Amy Hui-Mei; Hamaker, Bruce R.] Purdue Univ, Dept Food Sci, W Lafayette, IN 47907 USA. [Nichols, Buford L.; Rose, David R.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. [Jones, Kyra] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada. [Quezada-Calvillo, Roberto] Univ Autonoma San Luis Potosi, Dept Chem, Slp, Mexico. RP Hamaker, BR (reprint author), Purdue Univ, Dept Food Sci, Smith Hall, W Lafayette, IN 47907 USA. EM hamakerb@purdue.edu FU USDA AFRI competitive grant program [08-555-03-18793]; Canadian Institutes for Health Research [CIHR MOP111237]; Heart and Stoke Foundation of Ontario [NA-6305]; CIHR-Canadian Digestive Health Foundation studentship FX We thank the USDA AFRI competitive grant program, no. 08-555-03-18793, for financial support of this research. Funding was also provided by the Canadian Institutes for Health Research (CIHR MOP111237) and Heart and Stoke Foundation of Ontario (NA-6305). KJ was supported by a CIHR-Canadian Digestive Health Foundation studentship. NR 48 TC 4 Z9 4 U1 5 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD MAY PY 2014 VL 58 IS 5 BP 1111 EP 1121 DI 10.1002/mnfr.201300599 PG 11 WC Food Science & Technology SC Food Science & Technology GA AF5SO UT WOS:000334773400019 PM 24442968 ER PT J AU Luyssaert, S Jammet, M Stoy, PC Estel, S Pongratz, J Ceschia, E Churkina, G Don, A Erb, K Ferlicoq, M Gielen, B Grunwald, T Houghton, RA Klumpp, K Knohl, A Kolb, T Kuemmerle, T Laurila, T Lohila, A Loustau, D McGrath, MJ Meyfroidt, P Moors, EJ Naudts, K Novick, K Otto, J Pilegaard, K Pio, CA Rambal, S Rebmann, C Ryder, J Suyker, AE Varlagin, A Wattenbach, M Dolman, AJ AF Luyssaert, Sebastiaan Jammet, Mathilde Stoy, Paul C. Estel, Stephan Pongratz, Julia Ceschia, Eric Churkina, Galina Don, Axel Erb, KarlHeinz Ferlicoq, Morgan Gielen, Bert Gruenwald, Thomas Houghton, Richard A. Klumpp, Katja Knohl, Alexander Kolb, Thomas Kuemmerle, Tobias Laurila, Tuomas Lohila, Annalea Loustau, Denis McGrath, Matthew J. Meyfroidt, Patrick Moors, Eddy J. Naudts, Kim Novick, Kim Otto, Juliane Pilegaard, Kim Pio, Casimiro A. Rambal, Serge Rebmann, Corinna Ryder, James Suyker, Andrew E. Varlagin, Andrej Wattenbach, Martin Dolman, A. Johannes TI Land management and land-cover change have impacts of similar magnitude on surface temperature SO NATURE CLIMATE CHANGE LA English DT Article ID CLIMATE-CHANGE; VEGETATION; FEEDBACKS; FORESTS; ENERGY; CARBON; EARTH AB Anthropogenic changes to land cover (LCC) remain common, but continuing land scarcity promotes the widespread intensification of land management changes (LMC) to better satisfy societal demand for food, fibre, fuel and shelter(1). The biophysical effects of LCC on surface climate are largely understood(2-5), particularly for the boreal(6) and tropical zones(7), but fewer studies have investigated the biophysical consequences of LMC; that is, anthropogenic modification without a change in land cover type. Harmonized analysis of ground measurements and remote sensing observations of both LCC and LMC revealed that, in the temperate zone, potential surface cooling from increased albedo is typically offset by warming from decreased sensible heat fluxes, with the net effect being a warming of the surface. Temperature changes from LMC and LCC were of the same magnitude, and averaged 2 K at the vegetation surface and were estimated at 1.7 K in the planetary boundary layer. Given the spatial extent of land management (42-58% of the land surface) this calls for increasing the efforts to integrate land management in Earth System Science to better take into account the human impact on the climate(8). C1 [Luyssaert, Sebastiaan; McGrath, Matthew J.; Naudts, Kim; Otto, Juliane; Ryder, James] LSCE, F-91190 Gif Sur Yvette, France. [Jammet, Mathilde] Univ Copenhagen, Dept Geosci & Nat Resource Management, DK-1350 Copenhagen, Denmark. [Stoy, Paul C.; Kuemmerle, Tobias] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Estel, Stephan; Churkina, Galina] Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. [Pongratz, Julia; Ferlicoq, Morgan] Max Planck Inst Meteorol, D-20146 Hamburg, Germany. [Ceschia, Eric] Univ Toulouse 3, Ctr Etud Spatiales BIOsphere CESBIO, F-31401 Toulouse 9, France. [Don, Axel] Johann Heinrich von Thunen Inst, Inst Agr Climate Res, D-38116 Braunschweig, Germany. [Erb, KarlHeinz] Alpen Adria Univ Klagenfurt Vienna Graz, Inst Social Ecol Vienna SEC, A-1070 Vienna, Austria. [Gielen, Bert] Res Grp Plant & Vegetat Ecol, Dept Biol, B-2610 Antwerp, Belgium. [Gruenwald, Thomas] Tech Univ Dresden, Dept Meteorol Inst Hydrol & Meteorol, D-01737 Tharandt, Germany. [Houghton, Richard A.] Woods Hole Res Ctr, Falmouth, MA 02540 USA. [Klumpp, Katja] INRA, Grassland Ecosyst Res UREP, F-63100 Clermont Ferrand, France. [Knohl, Alexander] Univ Gottingen, D-37077 Gottingen, Germany. [Kolb, Thomas] No Arizona Univ, Sch Forestry, Flagsta, AZ 86011 USA. [Kuemmerle, Tobias] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Laurila, Tuomas; Lohila, Annalea] Finnish Meteorol Inst, FI-00101 Helsinki, Finland. [Loustau, Denis] INRA, Unite EPHYSE, F-33140 Villenave Dornon, France. [Meyfroidt, Patrick] Catholic Univ Louvain, FRS FNRS, Earth & Life Inst, B-1348 Louvain, Belgium. [Meyfroidt, Patrick] Catholic Univ Louvain, Georges Lemaitre Ctr Earth & Climate, Earth & Life Inst, B-1348 Louvain, Belgium. [Moors, Eddy J.] Alterra, NL-6700 AA Wageningen, Netherlands. [Novick, Kim] US Forest Serv, USDA, So Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. [Pilegaard, Kim] Tech Univ Denmark, Ctr Ecosyst & Environm Sustainabil, Dept Chem & Biochem Engn, DK-4000 Roskilde, Denmark. [Pio, Casimiro A.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal. [Pio, Casimiro A.] Univ Aveiro, Dept Environm, P-3810193 Aveiro, Portugal. [Rambal, Serge] CEFE CNRS, F-34293 Montpellier 5, France. [Rebmann, Corinna] Helmholtz Zentrum Umweltforsch GmbH UFZ, Dept Hydrosystemmodellierung, D-04318 Leipzig, Germany. [Suyker, Andrew E.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Varlagin, Andrej] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow 119071, Russia. [Wattenbach, Martin] GFZ German Res Ctr Geosci, Helmholtz Ctr Potsdam, Sect Hydrol 5 4, D-14473 Potsdam, Germany. [Dolman, A. Johannes] Vrije Univ Amsterdam, Dept Earth Sci, Earth & Climate cluster, NL-1081 HV Amsterdam, Netherlands. RP Luyssaert, S (reprint author), LSCE, F-91190 Gif Sur Yvette, France. EM Sebastiaan.Luyssaert@lsce.ipsl.fr RI Meyfroidt, Patrick/G-7768-2012; CESAM, UA/M-3762-2015; Luyssaert, Sebastiaan/F-6684-2011; Lohila, Annalea/C-7307-2014; ceschia, eric/P-5054-2014; Pio, Casimiro/A-8135-2008; Pilegaard, Kim/I-7137-2013; Knohl, Alexander/F-9453-2014; Don, Axel/A-1618-2009; OI Meyfroidt, Patrick/0000-0002-1047-9794; rambal, serge/0000-0001-5869-8382; Erb, Karlheinz/0000-0002-8335-4159; Lohila, Annalea/0000-0003-3541-672X; ceschia, eric/0000-0001-5941-752X; Pio, Casimiro/0000-0002-3531-8620; Pilegaard, Kim/0000-0002-5169-5717; Knohl, Alexander/0000-0002-7615-8870; Don, Axel/0000-0001-7046-3332; Luyssaert, Sebastiaan/0000-0003-1121-1869; Dolman, A.J./0000-0003-0099-0457 FU ERC [242564, 263522 LUISE]; FWO-Vlaanderen; Nordic Centre of Excellence; DEFROST, under the Nordic Top-Level Research Initiative; Center for Permafrost, CENPERM DNRF [100]; Einstein Foundation; European Commission [VOLANTE FP7-ENV-265104, FP-7226701, FP7-244122]; FEDER Interreg Iva [723 POCTEFA08/34]; ADEME; German Research Foundation (DFG) [SPP1257]; US NSF EF [1241881]; Marie Curie Incoming International Fellowship Programme; MT Institute on Ecosystems; European Commission through COST [ES0805]; IMECC Integrated Infrastructure Initiative (I3) project [026188] FX MODIS land surface temperature, black sky albedo, and the enhanced vegetation index were retrieved from the NASA Land Processes Distributed Active Archive Centre (LP DAAC, https://lpdaac.usgs.gov/). Site-level data were retrieved from the FLUXNET (http://daac.ornl.gov/), IMECC (http://gaia.agraria.unitus.it/) and AMERIFLUX (http://ameriflux.ornl.gov/) databases. Christophe Moisy prepared Supplementary Fig. 1. S.L. , M.J. , J.O. , M.J.M., K. Naudts and J.R. were funded through ERC starting grant 242564 and received additional funding through FWO-Vlaanderen. M.J. received funding also through the Nordic Centre of Excellence, DEFROST, under the Nordic Top-Level Research Initiative and the Center for Permafrost, CENPERM DNRF number 100. T.K. and S.E. were funded through the Einstein Foundation and the European Commission (VOLANTE FP7-ENV-265104). K.H.E. acknowledges funding from ERC starting grant 263522 LUISE. E.C. and M.F. received funding from the European Commission, FEDER Interreg Iva, 723 POCTEFA08/34 and ADEME. M.W. acknowledges funding from the German Research Foundation (DFG) through the SPP1257 priority program, and the European Commission FP-7226701 (CARBO-Extreme) and FP7-244122 (GHG-Europe), also for A.J.D. P.C.S. acknowledges funding from the US NSF EF #1241881, the Marie Curie Incoming International Fellowship Programme, and the MT Institute on Ecosystems. The authors acknowledge the financial help of the European Commission through COST ES0805 for organizing the Potsdam workshop in support of this study, and the IMECC Integrated Infrastructure Initiative (I3) project under the 6th Framework Program (contract number 026188). This study contributes to the Global Land Project (http://www.globallandproject.org). NR 30 TC 65 Z9 66 U1 19 U2 145 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD MAY PY 2014 VL 4 IS 5 BP 389 EP 393 DI 10.1038/NCLIMATE2196 PG 5 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AG4PV UT WOS:000335403500029 ER PT J AU Evans, RD Koyama, A Sonderegger, DL Charlet, TN Newingham, BA Fenstermaker, LF Harlow, B Jin, VL Ogle, K Smith, SD Nowak, RS AF Evans, R. D. Koyama, A. Sonderegger, D. L. Charlet, T. N. Newingham, B. A. Fenstermaker, L. F. Harlow, B. Jin, V. L. Ogle, K. Smith, S. D. Nowak, R. S. TI Greater ecosystem carbon in the Mojave Desert after ten years exposure to elevated CO2 SO NATURE CLIMATE CHANGE LA English DT Article ID SOIL ORGANIC-MATTER; ATMOSPHERIC CO2; NITROGEN DYNAMICS; AVAILABILITY; SEQUESTRATION; DECOMPOSITION; RESPIRATION; GRASSLAND; INCREASES; EMISSIONS AB Carbon dioxide is the main greenhouse gas inducing climate change. Increased global CO2 emissions, estimated at 8.4 Pg C yr(-1) at present, have accelerated from 1% yr(-1) during 1990-1999 to 2.5% yr(-1) during 2000-2009 (ref. 1). The carbon balance of terrestrial ecosystems is the greatest unknown in the global C budget because the actual magnitude, location and causes of terrestrial sinks are uncertain(2); estimates of terrestrial C uptake, therefore, are often based on the residuals between direct measurements of the atmospheric sink and well-constrained models of ocean uptake of CO2 (ref. 3). Here we report significant terrestrial C accumulation caused by CO2 enhancement to net ecosystem productivity in an intact, undisturbed arid ecosystem(4-8) following ten years of exposure to elevated atmospheric CO2. Results provide direct evidence that CO2 fertilization substantially increases ecosystem C storage and that arid ecosystems are significant, previously unrecognized, sinks for atmospheric CO2 that must be accounted for in efforts to constrain terrestrial and global C cycles. C1 [Evans, R. D.; Koyama, A.; Sonderegger, D. L.; Harlow, B.; Jin, V. L.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Evans, R. D.; Koyama, A.; Sonderegger, D. L.; Harlow, B.; Jin, V. L.] Washington State Univ, WSU Stable Isotope Core Facil, Pullman, WA 99164 USA. [Koyama, A.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Koyama, A.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Sonderegger, D. L.] Univ Arizona, Dept Math & Stat, Flagstaff, AZ 86011 USA. [Charlet, T. N.; Newingham, B. A.; Smith, S. D.] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA. [Newingham, B. A.] Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA. [Fenstermaker, L. F.] Desert Res Inst, Div Earth & Ecosyst Sci, Las Vegas, NV 89119 USA. [Jin, V. L.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Ogle, K.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Nowak, R. S.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. RP Evans, RD (reprint author), Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. EM rdevans@wsu.edu FU Department of Energy's Terrestrial Carbon Processes Program [DE-FG02-03ER63650, DEFG02-03ER63651]; NSF Ecosystem Studies Program [DEB-98-14358, 02-12819] FX The authors gratefully acknowledge grant support from the Department of Energy's Terrestrial Carbon Processes Program (DE-FG02-03ER63650, DEFG02-03ER63651) and the NSF Ecosystem Studies Program (DEB-98-14358 and 02-12819). We also acknowledge the DOE's National Nuclear Security Administration for providing utility services and undisturbed land at the Nevada National Security Site (formerly Nevada Test Site) to conduct the FACE experiment. We also thank S. Chung at WSU for advice on current emission inventories. NR 30 TC 12 Z9 13 U1 7 U2 59 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD MAY PY 2014 VL 4 IS 5 BP 394 EP 397 DI 10.1038/NCLIMATE2184 PG 4 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AG4PV UT WOS:000335403500030 ER PT J AU McNulty, SG Boggs, JL Sun, G AF McNulty, Steven G. Boggs, Johnny L. Sun, Ge TI The rise of the mediocre forest: why chronically stressed trees may better survive extreme episodic climate variability SO NEW FORESTS LA English DT Article DE Anthropogenic climate change; Adaptive management; Genetics; Tree; Forest; Inverse stress; Chronic; Stress; Episodic stress ID CRITICAL ACID LOADS; CARBON SEQUESTRATION; STAND DENSITY; UNITED-STATES; NITROGEN LIMITATION; PINE FORESTS; WATER-STRESS; DOUGLAS-FIR; RED SPRUCE; SOIL AB Anthropogenic climate change is a relatively new phenomenon, largely occurring over the past 150 years, and much of the discussion on climate change impacts to forests has focused on long-term shifts in temperature and precipitation. However, individual trees respond to the much shorter impacts of climate variability. Historically, fast growing, fully canopied, non-chronically stressed (NCS) trees (e.g., those with minimal insect or disease damage, growing on high nutrient, moist soils), have been considered the model of individual health, while slower growing, broken crown, chronically stressed (CS) trees growing on nutrient poor, or dry soils, were considered to be unhealthy and prone to stress related mortality. Over the coming years and decades, climate variability (e.g., extreme precipitation events, drought, heat waves) is expected to increase. We hypothesize that as the duration, severity and interaction of stress becomes more pronounced under increasing climate variability, seemingly less healthy, CS trees could begin to survive environmental stress better than their traditionally considered healthy, NCS tree counterparts. We further hypothesize that this shift in survivorship will be attributed to tree response to increasingly extreme climate variability at levels not previously observed in recorded history. While NCS trees can better survive historic episodic stress, we hypothesize that CS trees may be better suited to survive the combination of chronic low-level stress, and extreme episodic stress associated with anthropogenic climate change and increasing climate variability. Specifically, morphological differences such as increased root and reduced leaf mass, and physiological differences such as more oleoresin production, could make slower growing, CS forests more resilient to catastrophic episodic mortality compared to more NCS stands under certain circumstances. General circulation models suggest that climate variability will increase in the future. Therefore, the occurrence of slow growth, CS forest stands out surviving faster growing NCS less stressed stands may become more common place, and eventually lead to "The Age of the Mediocre Forest". This paper addresses the type of environmental conditions that could create such an inverse in forest health conditions, and the implications that this shift might have in forest genetics and management. C1 [McNulty, Steven G.; Boggs, Johnny L.; Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC 27606 USA. RP McNulty, SG (reprint author), US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, 920 Main Campus Dr, Raleigh, NC 27606 USA. EM steve_mcnulty@ncsu.edu FU USDA Forest Service Eastern Environmental Threat Assessment Center FX Funding for this study was provided by the USDA Forest Service Eastern Environmental Threat Assessment Center. The authors wish to thank Robert Doudrick and Hermann Gucinski for assistance in field work, and Eric Ward and two anonymous reviewers for helping to refine the theory. NR 50 TC 7 Z9 7 U1 3 U2 48 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 EI 1573-5095 J9 NEW FOREST JI New For. PD MAY PY 2014 VL 45 IS 3 SI SI BP 403 EP 415 DI 10.1007/s11056-014-9410-3 PG 13 WC Forestry SC Forestry GA AG7DD UT WOS:000335577300007 ER PT J AU Wallner, AM Hamilton, GC Nielsen, AL Hahn, N Green, EJ Rodriguez-Saona, CR AF Wallner, Adam M. Hamilton, George C. Nielsen, Anne L. Hahn, Noel Green, Edwin J. Rodriguez-Saona, Cesar R. TI Landscape Factors Facilitating the Invasive Dynamics and Distribution of the Brown Marmorated Stink Bug, Halyomorpha halys (Hemiptera: Pentatomidae), after Arrival in the United States SO PLOS ONE LA English DT Article ID HETEROPTERA-PENTATOMIDAE; PATTERNS; DISPERSAL; ABUNDANCE; SPREAD; CORN AB The brown marmorated stink bug, Halyomorpha halys, a native of Asia, has become a serious invasive pest in the USA. H. halys was first detected in the USA in the mid 1990s, dispersing to over 41 other states. Since 1998, H. halys has spread throughout New Jersey, becoming an important pest of agriculture, and a major nuisance in urban developments. In this study, we used spatial analysis, geostatistics, and Bayesian linear regression to investigate the invasion dynamics and colonization processes of this pest in New Jersey. We present the results of monitoring H. halys from 51 to 71 black light traps that were placed on farms throughout New Jersey from 2004 to 2011 and examined relationships between total yearly densities of H. halys and square hectares of 48 landscape/land use variables derived from urban, wetland, forest, and agriculture metadata, as well as distances to nearest highways. From these analyses we propose the following hypotheses: (1) H. halys density is strongly associated with urban developments and railroads during its initial establishment and dispersal from 2004 to 2006; (2) H. halys overwintering in multiple habitats and feeding on a variety of plants may have reduced the Allee effect, thus facilitating movement into the southernmost regions of the state by railroads from 2005 to 2008; (3) density of H. halys contracted in 2009 possibly from invading wetlands or sampling artifact; (4) subsequent invasion of H. halys from the northwest to the south in 2010 may conform to a stratified-dispersal model marked by rapid long-distance movement, from railroads and wetland rights-of-way; and (5) high densities of H. halys may be associated with agriculture in southern New Jersey in 2011. These landscape features associated with the invasion of H. halys in New Jersey may predict its potential rate of invasion across the USA and worldwide. C1 [Wallner, Adam M.] USDA, Anim & Plant Hlth Serv, Plant Protect Quarantine Plant Inspect Stn, Miami, FL 33158 USA. [Hamilton, George C.; Nielsen, Anne L.; Hahn, Noel; Rodriguez-Saona, Cesar R.] Rutgers State Univ, Dept Entomol, New Brunswick, NJ 08903 USA. [Green, Edwin J.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08903 USA. RP Wallner, AM (reprint author), USDA, Anim & Plant Hlth Serv, Plant Protect Quarantine Plant Inspect Stn, Miami, FL 33158 USA. EM adam.m.wallner@aphis.usda.gov FU U.S. Hatch Funds; U.S. Department of Agriculture - National Institute of Food and Agriculture - Specialty Crop Research Initiative Award [2011-51181-30937] FX Funding came from U.S. Hatch Funds and the U.S. Department of Agriculture - National Institute of Food and Agriculture - Specialty Crop Research Initiative Award #2011-51181-30937. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 9 Z9 10 U1 9 U2 60 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 1 PY 2014 VL 9 IS 5 AR e95691 DI 10.1371/journal.pone.0095691 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG6EE UT WOS:000335510600048 PM 24787576 ER PT J AU Kou, LP Gaskins, VL Luo, YG Jurick, WM AF Kou, L. P. Gaskins, V. L. Luo, Y. G. Jurick, W. M., II TI First Report of Fusarium avenaceum Causing Postharvest Decay of 'Gala' Apple Fruit in the United States SO PLANT DISEASE LA English DT News Item C1 [Kou, L. P.] Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. [Gaskins, V. L.; Luo, Y. G.; Jurick, W. M., II] USDA ARS, Food Qual Lab, Beltsville, MD USA. RP Kou, LP (reprint author), Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. NR 5 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 690 EP 690 DI 10.1094/PDIS-07-13-0803-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800034 ER PT J AU Green, HC Haugland, RA Varma, M Millen, HT Borchardt, MA Field, KG Walters, WA Knight, R Sivaganesan, M Kelty, CA Shanks, OC AF Green, Hyatt C. Haugland, Richard A. Varma, Manju Millen, Hana T. Borchardt, Mark A. Field, Katharine G. Walters, William A. Knight, R. Sivaganesan, Mano Kelty, Catherine A. Shanks, Orin C. TI Improved HF183 Quantitative Real-Time PCR Assay for Characterization of Human Fecal Pollution in Ambient Surface Water Samples SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID 16S RIBOSOMAL-RNA; MICROBIAL SOURCE TRACKING; GENETIC-MARKERS; FRESH-WATER; BACTEROIDES MARKERS; INDICATOR BACTERIA; QPCR; QUANTIFICATION; PERFORMANCE; AMPLIFICATION AB Quantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genus Bacteroides are among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters. C1 [Green, Hyatt C.; Sivaganesan, Mano; Kelty, Catherine A.; Shanks, Orin C.] US EPA, Off Res & Dev, Natl Risk Management & Res Lab, Cincinnati, OH 45268 USA. [Haugland, Richard A.; Varma, Manju] US EPA, Off Res & Dev, Natl Exposure Res Lab, Cincinnati, OH 45268 USA. [Millen, Hana T.] US Geol Survey, Wisconsin Water Sci Ctr, Marshfield, WI USA. [Borchardt, Mark A.] ARS, USDA, Marshfield, WI USA. [Field, Katharine G.] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA. [Walters, William A.] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA. [Knight, R.] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA. [Knight, R.] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA. RP Shanks, OC (reprint author), US EPA, Off Res & Dev, Natl Risk Management & Res Lab, Cincinnati, OH 45268 USA. EM shanks.orin@epa.gov RI Knight, Rob/D-1299-2010 FU NIGMS NIH HHS [T32 GM008759] NR 41 TC 25 Z9 25 U1 3 U2 33 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2014 VL 80 IS 10 BP 3086 EP 3094 DI 10.1128/AEM.04137-13 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AG4JQ UT WOS:000335386200015 PM 24610857 ER PT J AU Feng, ZX Baroch, JA Long, LP Xu, YF Cunningham, FL Pedersen, K Lutman, MW Schmit, BS Bowman, AS DeLiberto, TJ Wan, XF AF Feng, Zhixin Baroch, John A. Long, Li-Ping Xu, Yifei Cunningham, Frederick L. Pedersen, Kerri Lutman, Mark W. Schmit, Brandon S. Bowman, Andrew S. DeLiberto, Thomas J. Wan, Xiu-Feng TI Influenza A Subtype H3 Viruses in Feral Swine, United States, 2011-2012 SO EMERGING INFECTIOUS DISEASES LA English DT Article ID ANTIGENIC CHARACTERIZATION; AMERICAN PIGS AB To determine whether, and to what extent, influenza A subtype H3 viruses were present in feral swine in the United States, we conducted serologic and virologic surveillance during October 2011-September 2012. These animals were periodically exposed to and infected with A(H3N2) viruses, suggesting they may threaten human and animal health. C1 [Feng, Zhixin; Long, Li-Ping; Xu, Yifei; Wan, Xiu-Feng] Mississippi State Univ, Mississippi State, MS 39762 USA. [Feng, Zhixin] Jiangsu Acad Agr Sci, Nanjing, Jiangsu, Peoples R China. [Baroch, John A.; Pedersen, Kerri; Lutman, Mark W.; Schmit, Brandon S.; DeLiberto, Thomas J.] US Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO USA. [Cunningham, Frederick L.] US Anim & Plant Hlth Inspect Serv, USDA, Mississippi State, MS USA. [Bowman, Andrew S.] Ohio State Univ, Columbus, OH 43210 USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, 240 Wise Ctr Dr, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu RI Bowman, Andrew/B-4321-2012 OI Bowman, Andrew/0000-0002-0738-8453 FU US Department of Agriculture, Wildlife Services, National Wildlife Research Center [13-7428-0961-CA] FX This work was supported by the US Department of Agriculture, Wildlife Services, National Wildlife Research Center (grant no. 13-7428-0961-CA). NR 14 TC 6 Z9 6 U1 1 U2 12 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAY PY 2014 VL 20 IS 5 BP 843 EP 846 DI 10.3201/eid2005.131578 PG 4 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA AG0SC UT WOS:000335124900016 PM 24751326 ER PT J AU Yuan, LL Hao, MM Wu, LO Zhao, Z Rosenthal, BM Li, XM He, YS Sun, L Feng, GH Xiang, Z Cui, LW Yang, ZQ AF Yuan, Lili Hao, Mingming Wu, Lanou Zhao, Zhen Rosenthal, Benjamin M. Li, Xiaomei He, Yongshu Sun, Ling Feng, Guohua Xiang, Zheng Cui, Liwang Yang, Zhaoqing TI Refrigeration provides a simple means to synchronize in vitro cultures of Plasmodium falciparum SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Synchronization; Plasmodium falciparum; Refrigeration; Drug susceptibility ID GRADIENT MAGNETIC SEPARATION; INFECTED ERYTHROCYTES; SCHIZONTS; PARASITES; BLOOD; INHIBITOR; CELLS AB Plasmodium falciparum is usually asynchronous during in vitro culture. Highly synchronized cultures of P. falciparum are routinely used in malaria research. Here, we describe a simple synchronization procedure for P. falciparum asexual erythrocytic culture, which involves storage at 4 degrees C for 8-24 h followed by routine culture. When cultures with 27-60% of ring stage were synchronized using this procedure, 70-93% ring stages were obtained after 48 h of culture and relative growth synchrony remained for at least two erythrocytic cycles. To test the suitability of this procedure for subsequent work, drug sensitivity assays were perfornied using four laboratory strains and four freshly adapted clinical P. falciparum isolates. Parasites synchronized by sorbitol treatment or refrigeration showed similar dose-response curves and comparable IC50 values to four antimalarial drugs. The refrigeration synchronization method is simple, inexpensive, time-saving, and should be especially useful when large numbers of P. falciparum culture are handled. (C) 2014 Elsevier inc. All rights reserved. C1 [Yuan, Lili; Hao, Mingming; Zhao, Zhen; Sun, Ling; Xiang, Zheng] Kunming Med Univ, Dept Pathogen Biol & Immunol, Kunming 650500, Yunnan Province, Peoples R China. [Wu, Lanou; Zhao, Zhen; Yang, Zhaoqing] Kunming Med Univ, Dept Pharmacol, Kunming 650500, Yunnan Province, Peoples R China. [Rosenthal, Benjamin M.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Li, Xiaomei] Kunming Med Univ, Sch Publ Hlth, Kunming 650500, Yunnan Province, Peoples R China. [Feng, Guohua] Kunming Med Univ, Biomed Engn Res Ctr, Kunming 650500, Yunnan Province, Peoples R China. [He, Yongshu] Kunming Med Univ, Dept Cell Biol & Genet, Kunming 650500, Yunnan Province, Peoples R China. [Cui, Liwang] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. RP Cui, LW (reprint author), Kunming Med Univ, Dept Pathogen Biol & Immunol, 1168 West Chunrong Rd, Kunming 650500, Yunnan Province, Peoples R China. EM luc2@psu.edu; zhaoqingy@yahoo.com OI Rosenthal, Benjamin/0000-0002-0224-3773 FU National Institute of Health [U19 AI089672, 1R01AI075429]; National Science Foundation of China [U1202226, 81161120421, 31260508]; Doctor Science Research Foundation of the Education Ministry of China [20125317110001]; Yunnan Science Foundation [2012FB153] FX We thank Dr. Jun Miao for technical advice. This project was funded by the National Institute of Health (U19 AI089672 to L.C., 1R01AI075429 to Z.Y.), National Science Foundation of China (Nos. U1202226, 81161120421 and No. 31260508 to Z.Y.), the Doctor Science Research Foundation of the Education Ministry of China (No. 20125317110001 to Z.Y.) and Yunnan Science Foundation (No. 2012FB153 to Z.X.). NR 27 TC 2 Z9 3 U1 0 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 EI 1090-2449 J9 EXP PARASITOL JI Exp. Parasitol. PD MAY PY 2014 VL 140 BP 18 EP 23 DI 10.1016/j.exppara.2014.03.010 PG 6 WC Parasitology SC Parasitology GA AG3AR UT WOS:000335288700004 PM 24632190 ER PT J AU Batz, M Hoffmann, S Morris, JG AF Batz, Michael Hoffmann, Sandra Morris, J. Glenn, Jr. TI Disease-Outcome Trees, EQ-5D Scores, and Estimated Annual Losses of Quality-Adjusted Life Years (QALYs) for 14 Foodborne Pathogens in the United States SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID BURDEN; ILLNESS; INFECTIONS; PRIORITIZATION; RANKING AB Measures of disease burden such as quality-adjusted life years (QALYs) are increasingly important to risk-based food safety policy. They provide a means of comparing relative risk from diverse health outcomes. We present detailed disease-outcome trees and EQ-5D scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the United States (Campylobacter spp., Clostridium perfringens, Cryptosporidium parvum, Cyclospora cayetanensis, Escherichia coli O157:H7, Shiga toxin-producing E. coli non-O157, Listeria monocytogenes, nontyphoidal Salmonella enterica, Shigella, Toxoplasma gondii, Vibrio vulnificus, Vibrio parahaemolyticus and other noncholera Vibrio, and Yersinia enterocolitica). We estimate over 5800 QALYs lost per 1000 cases of L. monocytogenes and V. vulnificus, compared to 125 QALYs lost per 1000 cases of T. gondii, 26 for E. coli O157:H7, 16 for Salmonella and Campylobacter, and 14 for Y. enterocolitica. The remaining 7 pathogens are estimated to cause less than 5 QALYs lost per 1000 cases. In total, these 14 pathogens cause over 61,000 in QALY loss annually, with more than 90% due solely to acute infection being responsible for 65% of total QALY loss, with premature mortality and morbidity due to chronic and congenital illness responsible for another 28%. These estimates of the burden of chronic sequelae are likely conservative; additional epidemiological research is needed to support more accurate burden estimates. This study shows the value of using integrated metrics for comparing disease burden, and the need to consider chronic and congenital illness when prioritizing foodborne pathogens. C1 [Batz, Michael; Morris, J. Glenn, Jr.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA. [Hoffmann, Sandra] USDA, Econ Res Serv, Food Econ Div, Washington, DC USA. RP Batz, M (reprint author), Univ Florida, Emerging Pathogens Inst, POB 100009, Gainesville, FL 32610 USA. EM mbatz@UFL.edu FU Robert Wood Johnson Foundation; Cooperative State Research, Education, and Extension Service of the U.S. Department of Agriculture; Milbank Memorial Fund FX We thank Alan J. Krupnick, Paul S. Fischbeck, Heather Green, Michael McWilliams, and Michael R. Taylor for their contributions to this research. We also thank Douglas Archer, Paul Frenzen, Susan V. Grooters, Craig Hedberg, Lee-Ann Jaykus, Morris Potter, Elaine Scallan, Caroline Smith DeWaal, and Richard Williams for serving on our project advisory panel. This research was supported by the Robert Wood Johnson Foundation, the Cooperative State Research, Education, and Extension Service of the U.S. Department of Agriculture, and the Milbank Memorial Fund. NR 32 TC 9 Z9 9 U1 3 U2 20 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAY 1 PY 2014 VL 11 IS 5 BP 395 EP 402 DI 10.1089/fpd.2013.1658 PG 8 WC Food Science & Technology SC Food Science & Technology GA AG4TH UT WOS:000335412700011 PM 24588853 ER PT J AU Cicconi-Hogan, KM Belomestnykh, N Gamroth, M Ruegg, RL Tikofsky, L Schukken, YH AF Cicconi-Hogan, K. M. Belomestnykh, N. Gamroth, M. Ruegg, R. L. Tikofsky, L. Schukken, Y. H. TI Short communication: Prevalence of methicillin resistance in coagulase-negative staphylococci and Staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the United States SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy; organic; methicillin resistance; Staphylococcus ID TANK MILK; ANTIMICROBIAL SUSCEPTIBILITY; MULTIPLEX PCR; MECA HOMOLOG; RISK-FACTORS; HERDS; MANAGEMENT; GENE; IDENTIFICATION; PATTERNS AB The objective of this study was to evaluate the presence of methicillin-resistant Staphylococcus aureus and coagulase-negative Staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in New York, Wisconsin, and Oregon from March 2009 to May 2011. Due to recent publications reporting the presence mecC (a mecA homolog not detected by traditional mecA-based PCR methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery of methicillin-resistant organisms from bulk tank milk In fotal, 13 isolates were identified as methicillin resistant: Staph. aureus (n = 1), Staphylococcus sciuri (n = 5), Staphylococcus chromogenes (n = 2), Staphylococcus saprophyticus (n = 3), Staphylococcus agnetis (n = 1), and Macrococcus caseolyticus (n = 1). The single methicillin-resistant Staph. aureus isolate was identified from an organic farm in New York, for an observed 0.3% prevalence at the farm level. The methicillin-resistant coagulase-negative staphylococci prevalence was 2% in the organic population and 5% in the conventional population. We did not identify mecC in any of the isolates, from our population. Of interest was the relatively high number of methicillin-resistant Staph..sciuri recovered, as the number of isolates from our study was considerably higher than those recovered from other recent studies that also assessed milk samples. Our research suggests that the presence of a potential methicillin-resistant Staphylococcus reservoir in milk, and likely the dairy farm population in the United States, is independent of the organic or conventional production system. C1 [Cicconi-Hogan, K. M.; Belomestnykh, N.; Tikofsky, L.; Schukken, Y. H.] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. [Gamroth, M.] Oregon State Univ, Dept Anim Sci, Corvallis, OR 97331 USA. [Ruegg, R. L.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. RP Cicconi-Hogan, KM (reprint author), ARS, USDA, Russell Res Ctr, Poultry Proc & Swine Physiol Res Unit, Athens, GA 30605 USA. EM kmc277@cornell.edu OI Schukken, Ynte/0000-0002-8250-4194 FU USDA National Institute of Food and Agriculture (NIFA) Integrated Organic Program (Washington, DC) [2008-51106-19463] FX This study was supported by the USDA National Institute of Food and Agriculture (NIFA) Integrated Organic Program (Washington, DC; grant no. 2008-51106-19463, "Impact of Organic Management on Dairy Animal Health and Well-being") and by a gift from Food Animal Concerns Trust (Chicago, IL). We thank all the farms that participated in the study throughout New York, Vermont, Pennsylvania, Oregon, and Wisconsin. The authors also recognize the efforts of the laboratories at Quality Milk Production Services at Cornell University (Ithaca, NY). We also acknowledge the assistance of Roxann Richert (University of Wisconsin-Madison) and Katie Stiglbauer (Oregon State University, Corvallis) in the collection of field milk samples. Special thanks to Craig Altier (Cornell University, Ithaca NY) for his assistance with testing design and to Abhijit Gurjar (Merck Animal Health, Summit NJ) for his assistance with the RAPD assay. We acknowledge the use of the Staph. aureus MLST database, which is located at Imperial College London (London, UK) and is funded by the Wellcome Trust (London, UK). NR 33 TC 1 Z9 1 U1 4 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2014 VL 97 IS 5 BP 2959 EP 2964 DI 10.3168/jds.2013-7523 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AF4BE UT WOS:000334655600044 PM 24582450 ER PT J AU Hall, MB Chase, LE AF Hall, M. B. Chase, L. E. TI Responses of late-lactation cows to forage substitutes in low-forage diets supplemented with by-products SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE by-product; dairy cow; forage substitute; milk production; diet formulation ID NEUTRAL DETERGENT FIBER; FEED-INTAKE; REPLACEMENT; CATTLE AB In response to drought-induced forage shortages along with increased corn and soy prices, this study was conducted to evaluate lactation responses of dairy cows to lower-forage diets supplemented with forage substitutes. By-product feeds were used to completely replace corn grain and soybean feeds. Forty-eight late-lactation cows were assigned to 1 of 4 diets using a randomized complete block design with a 2-wk covariate period followed by a 4-wk experimental period. The covariate diet contained corn grain, soybean meal, and 61% forage. Experimental diets contained chopped wheat straw (WS)/sugar beet pulp at 0/12, 3/9, 6/6, or 9/3 percentages of diet dry matter (DM). Corn silage (20%), alfalfa silage (20%), pelleted corn gluten feed (25.5%), distillers grains (8%), whole cottonseed (5%), cane molasses/whey blend (7%), and vitamin and mineral mix with monensin (2.5%) comprised the rest of diet DM. The WS/sugar beet pulp diets averaged 16.5% crude protein, 35% neutral detergent fiber, and 11% starch (DM basis). Cows consuming the experimental diets maintained a 3.5% fat- and protein-corrected milk production (35.2 kg; standard deviation = 5.6 kg) that was numerically similar to that measured in the covariate period (35.3 kg; standard deviation = 5.0 kg). Intakes of DM and crude protein declined linearly as WS increased, whereas neutral detergent fiber intake increased linearly. Linear increases in time spent ruminating (from 409 to 502 min/d) and eating (from 156 to 223 min/d) were noted as WS inclusion increased. Yields of milk fat and 3.5% fat-and protein-corrected milk did not change as WS increased, but those of protein and lactose declined linearly. Phosphorous intakes were in excess of recommended levels and decreased linearly with increasing WS inclusion. Nutritional model predictions for multiparous cows were closest to actual performance for the National Research Council 2001 model when a metabolizable protein basis was used; primiparous cow performance was better predicted by energy-based predictions made with the National Research Council or Cornell Net Carbohydrate and Protein System models. Model predictions of performance showed a quadratic diet effect with increasing WS. Lactating dairy cows maintained production on low-forage diets that included forage substitutes, and in which by-product feeds fully replaced corn grain and soybean. However, longer-term studies are needed to evaluate animal performance and to improve model predictions of performance on these nontraditional diets. C1 [Hall, M. B.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Chase, L. E.] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA. RP Hall, MB (reprint author), USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM marybeth.hall@ars.usda.gov FU ADM Research (Decatur, IL) FX We thank ADM Research (Decatur, IL) for their donation of the corn gluten feed used in this study, R. D. Shaver of the University of Wisconsin (Madison) for his input on the planning of this study, and J. W. Pitas of the USDA Agricultural Research Service (Madison, WI) for technical support. NR 24 TC 4 Z9 4 U1 1 U2 24 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2014 VL 97 IS 5 BP 3042 EP 3052 DI 10.3168/jds.2013-7539 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AF4BE UT WOS:000334655600054 PM 24612800 ER PT J AU Cole, JB Waurich, B Wensch-Dorendorf, M Bickhart, DM Swalve, HH AF Cole, J. B. Waurich, B. Wensch-Dorendorf, M. Bickhart, D. M. Swalve, H. H. TI A genome-wide association study of calf birth weight in Holstein cattle using single nucleotide polymorphisms and phenotypes predicted from auxiliary traits SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE birth weight; quantitative trait loci; selection index; single nucleotide polymorphism ID DYSTOCIA TRANSMITTING ABILITY; FRIESIAN DAIRY-CATTLE; GESTATION LENGTH; CALVING TRAITS; GENETIC EVALUATION; UNITED-STATES; FUNCTIONAL TRAITS; MILK-PRODUCTION; PERINATAL-MORTALITY; US HOLSTEIN AB Previous research has found that a quantitative trait locus exists affecting calving and conformation traits on Bos taurus autosome 18 that may be related to increased calf birth weights, which are not routinely recorded in the United States. Birth weight data from large, intensively managed dairies in eastern Germany with management systems similar to those commonly found in the United States were used to develop a selection index predictor for predicted transmitting ability (PTA) of birth weight. The predictor included body depth, rump width, sire calving ease, sire gestation length, sire stillbirth, stature, and strength. Genetic and phenotypic correlations and heritabilities from the United States were substituted for the German values, and birth weight PTA predicted for 31,984 bulls with US genetic evaluations. A genome-wide association study was conducted on the predicted birth weight PTA with the 2-step genomic BLUP procedure used for routine evaluations in the United States. Allele substitution effects were predicted for 43,188 single nucleotide polymorphisms (SNP). Genotypes were available for 53,644 predictor animals Gene set enrichment analysis was performed on the 100 SNP that had the largest effects expressed in additive genetic standard deviations. Several SNP related to growth and development were found among the 25 SNP with the largest effects, including markers located within or near (<= 100 kbp) ABCA12, FLRT2, LHX4, MAP3K5, NRAC, NTNG1, PIGN, and ZNF75A. The gene set enrichment analysis identified the Kyoto Encyclopedia of Genes and Genomes "Regulation of actin cytoskeleton" pathway (bta04810) as being enriched. That pathway includes the ROCK gene, which is involved in placental function in the human, as well as other developmental genes (e.g., FAK and PAR). Prediction equations derived from one population are useful for identifying genes and gene networks associated with phenotypes that are not directly measured in a second population. This approach will identify only genes associated with the traits used to construct the birth weight predictor, and not loci that affect only birth weight. C1 [Cole, J. B.; Bickhart, D. M.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [Waurich, B.; Wensch-Dorendorf, M.; Swalve, H. H.] Univ Halle Wittenberg, Inst Agr & Nutr Sci, D-06120 Halle, Germany. RP Cole, JB (reprint author), USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. EM john.cole@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Bickhart, Derek/0000-0003-2223-9285 FU National Association of Animal Breeders (Columbia, MO); USDA Agricultural Research Service [1245-31000-101-00] FX The cooperation of the American Jersey Cattle Association (Reynoldsville, OH), Brown Swiss Association (Beloit, WI), and Holstein Association USA (Brattleboro, VT) in supplying pedigree data for registered cows and the dairy records processing centers [AgriTech Analytics (Visalia, CA), AgSource Cooperative Services (Verona, WI), Dairy Records Management Systems (Raleigh, NC, and Ames, IA), and DHI Computing Services (Provo, UT)] in supplying pedigree data for grade cows and calving event data is acknowledged. The National Association of Animal Breeders (Columbia, MO) contributed to funding the national calving traits evaluations. Holstein Association USA provided the US conformation evaluations used in this study. D. M. Bickhart and J. B. Cole were supported by the USDA Agricultural Research Service appropriated project 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." Two anonymous reviewers are thanked for their comments and suggestions, which improved the quality of the manuscript. NR 113 TC 13 Z9 14 U1 2 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2014 VL 97 IS 5 BP 3156 EP 3172 DI 10.3168/jds.2013-7409 PG 17 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AF4BE UT WOS:000334655600064 PM 24612794 ER PT J AU Gaddis, KLP Cole, JB Clay, JS Maltecca, C AF Gaddis, K. L. Parker Cole, J. B. Clay, J. S. Maltecca, C. TI Genomic selection for producer-recorded health event data in US dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy cattle; health; genomic selection ID SOMATIC-CELL SCORE; GENETIC-PARAMETERS; CLINICAL MASTITIS; HOLSTEIN COWS; FULL PEDIGREE; BREEDING PROGRAMS; MILK-PRODUCTION; TRAITS; PREDICTIONS; ANIMALS AB Emphasizing increased profit through increased dairy cow production has revealed a negative relationship of production with fitness and health traits. Decreased cow health can affect herd profitability through increased rates of involuntary culling and decreased or lost milk sales. The development of genomic selection methodologies, with accompanying substantial gains in reliability for low-heritability traits, may dramatically improve the feasibility of genetic improvement of dairy cow health. Producer-recorded health information may provide a wealth of information for improvement of dairy cow health, thus improving profitability. The principal objective of this study was to use health data collected from on-farm computer systems in the United States to estimate variance components and heritability for health traits commonly experienced by dairy cows. A single-step analysis was conducted to estimate genomic variance components and heritabilities for health events, including cystic ovaries, displaced abomasum, ketosis, lameness, mastitis, metritis, and retained placenta. A blended H matrix was constructed for a threshold model with fixed effects of parity and year-season and random effects of herd-year and sire. The single-step genomic analysis produced heritability estimates that ranged from 0.02 (standard deviation = 0.005) for lameness to 0.36 (standard deviation = 0.08) for retained placenta. Significant genetic correlations were found between lameness and cystic ovaries, displaced abomasum and ketosis, displaced abomasum and metritis, and retained placenta and metritis. Sire reliabilities increased, on average, approximately 30% with the incorporation of genomic data. From the results of these analyses; it was concluded that genetic selection for health traits using producer-recorded data are feasible in the United States, and that the inclusion of genomic data substantially improves reliabilities for these traits. C1 [Gaddis, K. L. Parker; Maltecca, C.] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Cole, J. B.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [Clay, J. S.] Dairy Records Management Syst, Raleigh, NC 27603 USA. RP Gaddis, KLP (reprint author), N Carolina State Univ, Dept Anim Sci, Box 7621, Raleigh, NC 27695 USA. EM klparke4@ncsu.edu RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Genus plc (Basingstoke, UK); Select Sires Inc. (Plain City, OH) FX The authors thank Dairy Records Management Systems (Raleigh, NC) and the USDA Agricultural Research Service Animal Improvement Programs Laboratory (Beltsville, MD) for providing the data, and Ignacy Misztal's group (Department of Animal and Dairy Science, University of Georgia, Athens) for providing software for the genomic analysis. Partial funding for Journal of Dairy Science Vol. 97 No. 5, 2014 this research was provided by Genus plc (Basingstoke, UK) and Select Sires Inc. (Plain City, OH). NR 53 TC 20 Z9 20 U1 3 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2014 VL 97 IS 5 BP 3190 EP 3199 DI 10.3168/jds.2013-7543 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AF4BE UT WOS:000334655600066 ER PT J AU Hutchison, JL Cole, JB Bickhart, DM AF Hutchison, J. L. Cole, J. B. Bickhart, D. M. TI Short communication: Use of young bulls in the United States SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE young bull; breeding; genomics AB The availability of genomic evaluations since 2008 has resulted in many changes to dairy cattle breeding programs. One such change has been the increased contribution of young bulls (0.8 to 3.9 yr old) to those programs The increased use of young bulls was investigated using pedigree data and breeding records obtained from the US national dairy database (Beltsville, MD). The adoption of genotyping was so rapid that by 2009, >90% of all Holstein artificial insemination (AI) service sires and 86% of Jersey AT service sires were genotyped, regardless of age. The percentage of sons sired by young bulls increased by 49 percentage points (10% in 2008 compared with 59% in 2012) due to the onset of genomic evaluations for Holsteins and by 46 percentage points for Jerseys (11 and 57%, respectively). When limiting these data to sons retained for breeding purposes through AI, the increase was even more dramatic, increasing approximately 80 percentage points from 2008 to 2012 for both Holsteins and Jerseys (1, 5, 28, 52, and 81% for Holsteins and 3, 4, 43, 46, and 82% for Jerseys from 2008 through 2012). From US breeding records from 2007 through 2012, 24,580,793 Holstein and 1,494,095 Jersey breedings were examined. Young bulls accounted for 28% and 25% of Holstein and Jersey breedings in 2007, respectively. These percentages increased to 51% of Holstein and 52% of Jersey breedings in 2012, representing 23- and 27-percentage-unit increases, respectively. Matings to genotyped young bulls have rapidly increased while the use of nongenotyped bulls has diminished since the onset of genomics. Mean sire age for Holstein male progeny born in 2012 was 2.7 yr younger than males born in 2006, and 1.3 yr younger for females; corresponding values for Jerseys were 2.3 and 0.9 yr. Holstein male offspring had an increase of 281 kg between 2006 and 2012, compared with 197 kg between 2000 and 2006 for parent averages (PA) for milk, an increase of 84 kg between the 2 periods. Jersey male offspring had an increase of 49 kg between the 2 periods. To demonstrate the economic impact of the differential use of young bulls, herds were grouped by the frequency of their use of young bulls, and average PTA for milk and net merit for cows that were bred in 2003 through 2012 were calculated. In 2012, herds. using >75% young bulls created offspring that had a PA of +52 kg for milk and +$58 net merit compared with herds using no young bulls. Jersey herds using >75% young bulls created offspring that had a PA of +142 kg for milk and +$63 for net merit compared with herds using no young bulls. Use of young bulls has greatly reduced the generation interval and improved the rate of genetic gain since the implementation of genomic evaluations. C1 [Hutchison, J. L.; Cole, J. B.; Bickhart, D. M.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. RP Hutchison, JL (reprint author), USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. EM Jana.Hutchison@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Bickhart, Derek/0000-0003-2223-9285 NR 9 TC 10 Z9 10 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2014 VL 97 IS 5 BP 3213 EP 3220 DI 10.3168/jds.2013-7525 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AF4BE UT WOS:000334655600068 PM 24612804 ER PT J AU Juneja, VK Mukhopadhyay, S Ukuku, D Hwang, CA Wu, VCH Thippareddi, H AF Juneja, Vijay K. Mukhopadhyay, Sudarsan Ukuku, Dike Hwang, Cheng-An Wu, Vivian C. H. Thippareddi, Harshavardhan TI Interactive Effects of Temperature, pH, and Water Activity on the Growth Kinetics of Shiga Toxin-Producing Escherichia coli O104:H4 SO JOURNAL OF FOOD PROTECTION LA English DT Article ID RESPONSE-SURFACE MODELS; SODIUM-CHLORIDE; STORAGE-TEMPERATURE; LABORATORY MEDIUM; PROCESSING PLANTS; UNITED-STATES; NON-O157; O157-H7; PREVALENCE; NITRITE AB The risk of non-O157 Shiga toxin producing Escherichia coli strains has become a growing public health concern. Several studies characterized the behavior of E. coli O157:H7; however, no reports on the influence of multiple factors on E. coli O104:H4 are available. This study examined the effects and interactions of temperature (7 to 46 degrees C), pH (4.5 to 8.5), and water activity (a(w); 0.95 to 0.99) on the growth kinetics of E. coli O104:H4 and developed predictive models to estimate its growth potential in foods. Growth kinetics studies for each of the 23 variable combinations from a central composite design were performed. Growth data were used to obtain the lag phase duration (LPD), exponential growth rate, generation time, and maximum population density (MPD). These growth parameters as a function of temperature, pH, and a(w) as controlling factors were analyzed to generate second-order response surface models. The results indicate that the observed MPD was dependent on the pH, a(w), and temperature of the growth medium. Increasing temperature resulted in a concomitant decrease in LPD. Regression analysis suggests that temperature, pH, and a(w) significantly affect the LPD, exponential growth rate, generation time, and MPD of E. coli 0104:H4. A comparison between the observed values and those of E. coli O157:H7 predictions obtained by using the U.S. Department of Agriculture Pathogen Modeling Program indicated that E. coli O104:H4 grows faster than E. coli O157:H7. The developed models were validated with alfalfa and broccoli sprouts. These models will provide risk assessors and food safety managers a rapid means of estimating the likelihood that the pathogen, if present, would grow in response to the interaction of the three variables assessed. C1 [Juneja, Vijay K.; Mukhopadhyay, Sudarsan; Ukuku, Dike; Hwang, Cheng-An] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Wu, Vivian C. H.] Univ Maine, Sch Food & Agr, Orono, ME 04469 USA. [Thippareddi, Harshavardhan] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. RP Juneja, VK (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM vijay.juneja@ars.usda.gov NR 28 TC 2 Z9 2 U1 1 U2 39 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2014 VL 77 IS 5 BP 706 EP 712 DI 10.4315/0362-028X.JFP-13-387 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AG5AX UT WOS:000335432600002 PM 25198132 ER PT J AU Wang, R Koohmaraie, M Luedtke, BE Wheeler, TL Bosilevac, JM AF Wang, Rong Koohmaraie, Mohammad Luedtke, Brandon E. Wheeler, Tommy L. Bosilevac, Joseph M. TI Effects of In-Plant Interventions on Reduction of Enterohemorrhagic Escherichia coli and Background Indicator Microorganisms on Veal Calf Hides SO JOURNAL OF FOOD PROTECTION LA English DT Article ID BEEF PROCESSING PLANT; AEROBIC-BACTERIA; UNITED-STATES; ANTIMICROBIAL INTERVENTIONS; FRESH BEEF; HOT-WATER; PREVALENCE; O157-H7; CATTLE; ENTEROBACTERIACEAE AB Enterohemorrhagic Escherichia coli (EHEC) serotypes in veal have recently been recognized as a problem. Because hides are considered to be the principal source of EHEC and hide interventions have been shown to be very efficacious in the control of EHEC in beef processing plants, various hide-directed intervention strategies have been implemented in several veal processing plants to mitigate contamination. We evaluated the effectiveness of three different hide interventions used at veal processing plants: A, a water rinse followed by a manual curry comb of the hide; B, application of 200 ppm of chlorine followed by a hot water rinse; and C, a 5-min treatment with chlorine foam followed by a rinse with 180 to 200 ppm of acidified sodium chlorite. The levels of total aerobic bacteria, Enterobacteriaceae, coliforms, and E. coli, as well as the prevalence of Salmonella, E. coli O157:H7, and non-O157 EHEC, were determined on hides pre- and postintervention. Interventions A, B, and C reduced indicator organisms (P < 0.05) by 0.8 to 3.5 log CFU, 2.1 to 2.7 log CFU, and 1.0 to 1.5 log CFU, respectively. No Salmonella was detected on hides prior to intervention. E. coli O157:H7 prevalence was observed at only one plant, so comparison was not possible. Other non-O157 EHECs (O26, O103, and O111) were observed for all interventions studied. Interventions A and B reduced culture-confirmed non-O157 EHEC by 29 and 21 % , respectively, whereas intervention C did not reduce non-O157 EHEC. Our results show that the most effective veal hide intervention for reducing indicator organisms and EHECs was the application of 200 ppm of chlorine followed by hot water rinse. These data provide options that veal processors can consider in their EHEC control program. C1 [Wang, Rong; Luedtke, Brandon E.; Wheeler, Tommy L.; Bosilevac, Joseph M.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Koohmaraie, Mohammad] IEH Labs & Consulting Grp, Lake Forest Park, WA 98155 USA. [Koohmaraie, Mohammad] King Saud Univ, Coll Food & Agr, Riyadh 11451, Saudi Arabia. RP Wang, R (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM rong.wang@ars.usda.gov NR 35 TC 7 Z9 7 U1 0 U2 3 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2014 VL 77 IS 5 BP 745 EP 751 DI 10.4315/0362-028X.JFP-13-388 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AG5AX UT WOS:000335432600007 PM 24780328 ER PT J AU Palencia, ER Mitchell, TR Snook, ME Glenn, AE Gold, S Hinton, DM Riley, RT Bacon, CW AF Palencia, Edwin R. Mitchell, Trevor R. Snook, Maurice E. Glenn, Anthony E. Gold, Scott Hinton, Dorothy M. Riley, Ronald T. Bacon, Charles W. TI Analyses of Black Aspergillus Species of Peanut and Maize for Ochratoxins and Fumonisins SO JOURNAL OF FOOD PROTECTION LA English DT Article ID FUJIKUROI MATING POPULATION; FUSARIUM-VERTICILLIOIDES; A PRODUCTION; NIGER; STRAINS; GRAPES; GROWTH; CARCINOGENESIS; TEMPERATURE; CARBONARIUS AB The genus Aspergillus section Nigri, or the black aspergilli, represents genetically closely related species that produce the mycotoxins, ochratoxins and the fumonisins. Fumonisin B-1 (FB1) is of an added concern because it is also a virulence factor for maize. Our preliminary data indicated that black aspergilli could develop asymptomatic infections with maize and peanuts plants. Symptomless infections are potential problems, because under favorable conditions, there is a potential for accumulation of ochratoxins and the fumonisins in contaminated postharvest crops. In the present report, the ability of black aspergilli from peanuts and maize to produce ochratoxin A and FB1 on maize kernels was assessed. One hundred fifty strains from peanuts and maize were isolated from several southeastern and midwestern states. Aspergillus nigri (A. nigri var. nigri) was the dominant species (87%), while Aspergillus foetidus, Aspergillus japonicus, Aspergillus tubingensis, and Aspergillus carbonarius were infrequently isolated. None of the wild isolates produced detectable amounts of ochratoxins. However, we do report the occurrence of the fumonisins B-1, B-2, and B-3. Of 54 field isolates, 30% (n = 16) produced FB1, 61% (n = 33) produced FB2, and 44% (n = 24) produced FB3. The amounts of fumonisins produced during the test period of 30 days suggest that these strains might be weak to moderate producers of fumonisin on maize. To our knowledge, this is a first report of FB1 and FB3 production by isolates of black aspergilli from an American cereal and legume. C1 [Palencia, Edwin R.; Mitchell, Trevor R.; Snook, Maurice E.; Glenn, Anthony E.; Gold, Scott; Hinton, Dorothy M.; Riley, Ronald T.; Bacon, Charles W.] ARS, USDA, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [Palencia, Edwin R.] Univ Georgia, Dept Plant Pathol, Athens, GA 30604 USA. RP Bacon, CW (reprint author), ARS, USDA, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. EM charles.bacon@ars.usda.gov NR 60 TC 6 Z9 6 U1 1 U2 26 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2014 VL 77 IS 5 BP 805 EP 813 DI 10.4315/0362-028X.JFP-13-321 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AG5AX UT WOS:000335432600015 PM 24780336 ER PT J AU Feng, PCH Reddy, SP AF Feng, Peter C. H. Reddy, Shanker P. TI Prevalence and Diversity of Enterotoxigenic Escherichia coli Strains in Fresh Produce SO JOURNAL OF FOOD PROTECTION LA English DT Article ID E. COLI; COLONIZATION FACTOR; VIRULENCE FACTORS; TOXIN GENES; EPIDEMIOLOGY; PATHOTYPES; BACTERIA; CATTLE; MEXICO; FOOD AB Analysis of fresh produce showed that enterotoxigenic Escherichia coli (ETEC) strains are most often found in cilantro and parsley, with prevalence rates of approximately 0.3%. Some ETEC strains also carried Shiga toxigenic E. coli (STEC) genes but had no STEC adherence factors, which are essential to cause severe human illness. Most ETEC strains in produce carried stable toxin and/or labile toxin genes but belonged to unremarkable serotypes that have not been reported to have caused human illnesses. C1 [Feng, Peter C. H.] US FDA, Div Microbiol, College Pk, MD 20740 USA. [Reddy, Shanker P.] Agr Mkt Serv, USDA, Washington, DC 20250 USA. RP Feng, PCH (reprint author), US FDA, Div Microbiol, College Pk, MD 20740 USA. EM peter.feng@fda.hhs.gov NR 25 TC 3 Z9 3 U1 3 U2 9 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2014 VL 77 IS 5 BP 820 EP 823 DI 10.4315/0362-028X.JFP-13-412 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AG5AX UT WOS:000335432600017 PM 24780338 ER PT J AU Berrang, ME Cox, NA Oakley, BB AF Berrang, Mark E. Cox, Nelson A. Oakley, Brian B. TI Sampling by Sponge Wipe or Skin Excision for Recovery of Inoculated Salmonella and Campylobacter from Defeathered Broiler Carcasses SO JOURNAL OF FOOD PROTECTION LA English DT Article ID CONTAMINATION; MICROORGANISMS; NUMBERS; FECES; SPP. AB Broilers may carry Salmonella and Campylobacter on inner and outer surfaces upon arrival at the slaughter plant, and carcasses can be further contaminated during commercial processing. A sensitive, nondestructive, repeatable sampling method would be useful to test carcasses for levels of bacteria before and after specific processing steps to measure either contamination or efficacy of intervention techniques. Blending of excised skin is accepted as an effective sampling method but requires damage to the carcass; this makes repeated measurements on the same carcass difficult. Herein we compare sponge sampling to skin excision to recover inoculated Salmonella and Campylobacter from broiler carcasses. In each of three replications, broiler carcass. breast skin was inoculated with approximately 6.0 log antimicrobial-resistant Salmonella and Campylobacter, allowed to dry for 60 s, and sampled by either sponge, skin excision, or sponge followed by skin excision. Antimicrobial-resistant Salmonella and Campylobacter were enumerated from all samples. Skin excision allowed recovery of 0.1 to 0.2 log more inoculated bacteria than did sponge sampling. When excision was used on the same skin previously sampled by sponging, the combination of both methods did not significantly improve recovery compared with sponging alone. Skin excision is slightly more sensitive than sponge sampling; however, for repeated nondestructive sampling of broiler carcasses during processing, sponge sampling may be preferable to recover Salmonella and Campylobacter within 60 s of a contamination event. C1 [Berrang, Mark E.; Cox, Nelson A.; Oakley, Brian B.] ARS, USDA, Russell Res Ctr, Athens, GA 30605 USA. RP Berrang, ME (reprint author), ARS, USDA, Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM mark.berrang@ars.usda.gov NR 19 TC 2 Z9 2 U1 0 U2 1 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2014 VL 77 IS 5 BP 824 EP 827 DI 10.4315/0362-028X.JFP-13-403 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AG5AX UT WOS:000335432600018 PM 24780339 ER PT J AU Gil-Zamorano, J Martin, R Daimiel, L Richardson, K Giordano, E Nicod, N Garcia-Carrasco, B Soares, SMA Iglesias-Gutierrez, E Lasuncion, MA Sala-Vila, A Ros, E Ordovas, JM Visioli, F Davalos, A AF Gil-Zamorano, Judit Martin, Roberto Daimiel, Lidia Richardson, Kris Giordano, Elena Nicod, Nathalie Garcia-Carrasco, Belen Soares, Sara M. A. Iglesias-Gutierrez, Eduardo Lasuncion, Miguel A. Sala-Vila, Aleix Ros, Emilio Ordovas, Jose M. Visioli, Francesco Davalos, Alberto TI Docosahexaenoic Acid Modulates the Enterocyte Caco-2 Cell Expression of MicroRNAs Involved in Lipid Metabolism SO JOURNAL OF NUTRITION LA English DT Article ID POLYUNSATURATED FATTY-ACID; DIET-INDUCED OBESITY; ENRICHMENT ANALYSIS; CANCER-CELLS; CHOLESTEROL; GROWTH; MICE; OMEGA-3-FATTY-ACIDS; ATHEROSCLEROSIS; ANGIOGENESIS AB Consumption of the long-chain omega-3 (n-3) polyunsaturated fatty acid docosahexaehoic acid (DHA) is associated with a reduced risk of cardiovascular disease and greater chemoprevention. However, the mechanisms underlying the biologic effects of DHA remain unknown. It is well known that microRNAs (miRNAs) are versatile regulators of gene expression. Therefore, we aimed to determine lithe beneficial effects of DHA may be modulated in part through miRNAs. Loss of dicer 1 ribonuclease type III (DICER) in enterocyte Caco-2 cells supplemented with DHA suggested that several lipid metabolism genes are modulated by miRNAs. Analysis of miRNAs predicted to target these genes revealed several miRNA candidates that are differentially modulated by fatty acids. Among the miRNAs modulated by DHA were miR-192 and miR-30c. Overexpression of either miR-192 or miR-30c in enterocyte and hepatocyte cells suggested an effect on the expression of genes related to lipid metabolism, some of which were confirmed by endogenous inhibition of these miRNAs. Our results show in enterocytes that DHA exerts its biologic effect in part by regulating genes involved in lipid metabolism and cancer. Moreover, this response is mediated through miRNA activity. We validate novel targets of miR-30c and miR-192 related to lipid metabolism and cancer including nuclear receptor corepressor 2, isocitrate dehydrogenase 1, DICER, caveolin 1, ATP-binding cassette subfamily G (white) member 4, retinoic acid receptor beta, and others. We also present evidence that in enterocytes DHA modulates the expression of regulatory factor X6 through these miRNAs. Alteration of miRNA levels by dietary components in support of their pharmacologic modulation might be valuable in adjunct therapy for dyslipidemia and other related diseases. C1 [Gil-Zamorano, Judit; Martin, Roberto; Daimiel, Lidia; Giordano, Elena; Nicod, Nathalie; Garcia-Carrasco, Belen; Soares, Sara M. A.; Visioli, Francesco; Davalos, Alberto] Madrid Inst Adv Studies Food, Lab Funct Foods, Madrid, Spain. [Richardson, Kris; Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Iglesias-Gutierrez, Eduardo] Univ Oviedo, Dept Funct Biol, Asturias, Spain. [Lasuncion, Miguel A.] Hosp Ramon & Cajal, Serv Bioquim Invest, IRYCIS, E-28034 Madrid, Spain. [Lasuncion, Miguel A.; Sala-Vila, Aleix; Ros, Emilio] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid, Spain. [Sala-Vila, Aleix; Ros, Emilio] Hosp Clin Barcelona, Inst Invest Biomed August Pi & Sunyer, Lipid Clin, Endocrinol & Nutr Serv, Barcelona, Spain. RP Davalos, A (reprint author), Madrid Inst Adv Studies Food, Lab Funct Foods, Madrid, Spain. EM alberto.davalos@imdea.org RI Lasuncion, Miguel A./K-6167-2014; Daimiel-Ruiz, Lidia Angeles/M-7779-2014; Visioli, Francesco/J-9356-2013; Martin-Hernandez, Roberto/M-8161-2014; OI Lasuncion, Miguel A./0000-0003-0299-9391; Daimiel-Ruiz, Lidia Angeles/0000-0001-9898-6629; Visioli, Francesco/0000-0002-1756-1723; Martin-Hernandez, Roberto/0000-0002-2723-6289; Davalos, Alberto/0000-0001-5709-6443 FU Institut de Salud Carlos III (FIS) [PI11/003151]; Sociedad Espanola de Arteriosclerosis; Ministerio de Economia y Competrtividad [AGL2011-28995, SAF2011-29951]; USDA FX Supported in part by the Institut de Salud Carlos III (FIS, PI11/003151 and the Sociedad Espanola de Arteriosclerosis (to A.D.), by the Ministerio de Economia y Competrtividad (AGL2011-28995 to F.V. and SAF2011-29951 to M.A.L), and by the USDA (to J.M.O.). The CIBER de Fisiopatologia de Is Obesidad y Nutricion is an initiative of Instituto de Salud Carlos III (ISCIII). NR 44 TC 13 Z9 13 U1 3 U2 18 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 575 EP 585 DI 10.3945/jn.113.189050 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700002 PM 24623846 ER PT J AU Ip, BC Liu, C Smith, DE Ausman, LM Wang, XD AF Ip, Blanche C. Liu, Chun Smith, Donald E. Ausman, Lynne M. Wang, Xiang-Dong TI High-Refined-Carbohydrate and High-Fat Diets Induce Comparable Hepatic Tumorigenesis in Male Mice SO JOURNAL OF NUTRITION LA English DT Article ID ENDOPLASMIC-RETICULUM STRESS; LIVER-DISEASE; HEPATOCELLULAR-CARCINOMA; NONALCOHOLIC STEATOHEPATITIS; GLYCEMIC INDEX; ER STRESS; KAPPA-B; TRANSLATION; ACTIVATION; PHOSPHORYLATION AB Previous studies demonstrated that diet-induced obese mice fed a semi-purified high-fat diet (HFD) had greater liver tumorigenesis than mice fed a non-semi-purified diet. Because ingredients present in standard unpurified diets may elicit potential chemopreventive properties that are not present in semi-purified diets, the present study evaluated hepatic tumorigenic effects of dietary fat by replacing it with refined carbohydrates [digestible saccharides; high-carbohydrate diet (HOD)] in a semi-purified diet without altering other components. Two-wk-old C57BI/6J male mice were randomly injected i.p. with either the liver-specific carcinogen diethylnitrosamine (25 mg/kg body weight) to induce liver cancer or saline as the nontumor control. At ages wk, mice with or without cancer initiation were further randomly assigned to an HFD (26% and 60% energy from carbohydrates and fat, respectively) or an HOD (66% and 12% energy from carbohydrates and fat, respectively) and consumed food ad libitum for 24 wk. Results showed that HOD-fed mice had a comparable degree of hepatic tumorigenesis (tumor number and volume) as HFD-fed mice, despite having significantly reduced body weights. HOD feeding induced greater hepatic endoplasmic reticulum (ER) stress-mediated protein kinase RNA-activated-like kinase (PERK) activation and oncogenic interleukin-6/signal transducer and activator of transcription 3 signaling than HFD feeding. HOD-stimulated PERK signaling was associated with elevated expression of prosurvival markers in tumors, including induced protein kinase B activation, increased extracellular signal-regulated kinases 1/2 phosphorylation, and elevated cyclin D1 protein expression. However, HOD-mediated PERK activation in tumors was also positively associated with markers of proapoptosis, which included elevated CCAAT/enhancer-binding protein homology protein expression and increased cleaved caspase-3. HCD-fed mice had greater severity in hepatic steatosis than HFD-fed mice. HCD-induced steatosis exacerbation was associated with increased expression in hepatic de novo lipogenic markers that can promote ER stress. Together, these data indicated that chronic HOD consumption by mice can produce comparable severity of hepatic tumorigenesis as HFD consumption, potentially through upregulating PERK-mediated ER stress. C1 [Ip, Blanche C.; Liu, Chun; Ausman, Lynne M.; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Boston, MA 02111 USA. [Smith, Donald E.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Comparat Biol Unit, Boston, MA 02111 USA. [Ip, Blanche C.; Ausman, Lynne M.; Wang, Xiang-Dong] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Wang, XD (reprint author), Tufts Univ, Nutr & Canc Biol Lab, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu OI IP, Blanche C/0000-0003-0063-5535 FU NIH [CA104932]; USDA/Agricultural Research Service [1950-51000-074S]; NIH/National Heart, Lung, and Blood Institute [5T32HL069772-10, 2T32HL069772-11A1] FX Supported by NIH grant CA104932, USDA/Agricultural Research Service grant 1950-51000-074S, and NIH/National Heart, Lung, and Blood Institute grants 5T32HL069772-10 and 2T32HL069772-11A1. Any opinions, findings, conclusions, and recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the sponsors. NR 36 TC 6 Z9 6 U1 1 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 647 EP 653 DI 10.3945/jn.113.189613 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700011 PM 24647392 ER PT J AU Taylor, CL Patterson, KY Roseland, JM Wise, SA Merkel, JM Pehrsson, PR Yetley, EA AF Taylor, Christine L. Patterson, Kristine Y. Roseland, Janet M. Wise, Stephen A. Merkel, Joyce M. Pehrsson, Pamela R. Yetley, Elizabeth A. TI Including Food 25-Hydroxyvitamin D in Intake Estimates May Reduce the Discrepancy between Dietary and Serum Measures of Vitamin D Status SO JOURNAL OF NUTRITION LA English DT Article ID TANDEM MASS-SPECTROMETRY; BIOLOGICAL-ACTIVITY; MAJOR METABOLITES; D-3; FORTIFICATION; TRANSPORT; PRESSURE; ADULTS AB The discrepancy between the commonly used vitamin D status measures intake and serum 25-hydroxyvitamin D [25(OH)D] concentrations has been perplexing. Sun exposure increases serum 25(OH)D concentrations and is often used as an explanation for the higher population-based serum concentrations in the face of apparently low vitamin D intake. However, sun exposure may not be the total explanation. 25(OH)D, a metabolite of vitamin D, is known to be present in animal-based foods. It has been measured and reported only sporadically and is not currently factored into U.S. estimates of vitamin D intake. Previously unavailable preliminary USDA data specifying the 25(OH)D content of a subset of foods allowed exploration of the potential change in the reported overall vitamin D content of foods when the presence of 25(OH)D was included. The issue of 25(OH)D potency was addressed, and available commodity intake estimates were used to outline trends in projected vitamin D intake when 25(OH)D in foods was taken into account. Given the data available, there were notable increases in the total vitamin D content of a number of animal-based foods when potency-adjusted 25(OH)D was included, and in turn there was a potentially meaningful increase (1.7-2.9 mu g or 15-30% of average requirement) in vitamin D intake estimates. The apparent increase could reduce discrepancies between intake estimates and serum 25(OH)D concentrations. The relevance to dietary interventions is discussed, and the need for continued exploration regarding 25(OH)D measurement is highlighted. C1 [Taylor, Christine L.; Merkel, Joyce M.; Yetley, Elizabeth A.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Patterson, Kristine Y.; Roseland, Janet M.; Pehrsson, Pamela R.] ARS, Nutr Data Lab, USDA, Beltsville, MD USA. [Wise, Stephen A.] NIST, Dept Commerce, Gaithersburg, MD 20899 USA. RP Taylor, CL (reprint author), NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. EM TaylorCL3@od.nih.gov NR 34 TC 18 Z9 18 U1 1 U2 8 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 654 EP 659 DI 10.3945/jn.113.189811 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700012 PM 24623845 ER PT J AU Hsu, JW Badaloo, A Wilson, L Taylor-Bryan, C Chambers, B Reid, M Forrester, T Jahoor, F AF Hsu, Jean W. Badaloo, Asha Wilson, Lorraine Taylor-Bryan, Carolyn Chambers, Bentley Reid, Marvin Forrester, Terrence Jahoor, Farook TI Dietary Supplementation with Aromatic Amino Acids Increases Protein Synthesis in Children with Severe Acute Malnutrition SO JOURNAL OF NUTRITION LA English DT Article ID ERYTHROCYTE GLUTATHIONE SYNTHESIS; BODY LEUCINE KINETICS; ENERGY MALNUTRITION; ACUTE-PHASE; INFECTION; IMPROVES; STATES AB Although 2 earlier studies reported that aromatic amino acid (AAA) supplementation of children with severe acute malnutrition (SAM) improved whole-body protein anabolism during the early postadmission (maintenance) phase of rehabilitation, it is not known whether this positive effect was maintained during the catch-up growth and recovery phases of treatment. This study aimed to determine whether supplementation with an AAA cocktail (330 mg kg(-1) . d(-1)) vs. isonitrogenous Ala would improve measures of protein kinetics in 22 children, aged 4-31 mo, during the catch-up growth and recovery phases of treatment for SAM. Protein kinetics were assessed by measuring leucine, phenylalanine, and urea kinetics with the use of standard stable isotope tracer methods in the fed state. Supplementation started at the end of the maintenance period when the children were clinically/metabolically stable and continued up to full nutritional recovery. Three experiments were performed: at the end of maintenance (at similar to 13 d postadmission), at mid-catch-up growth (at similar to 23 d postadmission when the children had replenished 50% of their weight deficit), and at recovery (at similar to 48 d postadmission when they had achieved at least 90% weight for length). Children in the AAA group had significantly faster protein synthesis compared with those in the Ala group at mid-catch-up growth (101 +/- 10 vs. 72 +/- 7 mu mol phenylalanine . kg(-1) . h(-1); P < 0.05) and better protein balance at mid-catch-up growth (49 +/- 5 vs. 30 +/- 2 mu mol phenylalanine . kg(-1) . h(-1); P < 0.05) and at recovery (37 +/- 8 vs. 11 +/- 3 mu mol phenylalanine . kg(-1) . h(-1); P < 0.05). We conclude that dietary supplementation with AAA accelerates net protein synthesis in children during nutritional rehabilitation for SAM, C1 [Hsu, Jean W.; Jahoor, Farook] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. [Badaloo, Asha; Wilson, Lorraine; Taylor-Bryan, Carolyn; Chambers, Bentley; Reid, Marvin; Forrester, Terrence] Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. RP Jahoor, F (reprint author), Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. EM fjahoor@bcm.edu OI Reid, Marvin/0000-0003-4005-9384 FU NIH [1RO1 DK 075018]; USDA Agricultural Research Service [58-6250-6001] FX Supported by NIH grant 1RO1 DK 075018 and with federal funds from the USDA Agricultural Research Service under cooperative agreement 58-6250-6001. NR 15 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 660 EP 666 DI 10.3945/jn.113.184523 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700013 PM 24647391 ER PT J AU Sahyoun, NR Nord, M Sassine, AJ Seyfert, K Hwalla, N Ghattas, H AF Sahyoun, Nadine R. Nord, Mark Sassine, Anniebelle J. Seyfert, Karin Hwalla, Nahla Ghattas, Hala TI Development and Validation of an Arab Family Food Security Scale SO JOURNAL OF NUTRITION LA English DT Article ID INSECURITY; PREVALENCE; CAMPINAS; HUNGER; BRAZIL; MODULE AB The objective of this article was to describe 1) the validation of 2 similar but not identical food security modules used to collect data from 2 vulnerable populations, southern Lebanon residents (n = 815) and Palestinian refugees in Lebanon (n = 2501), and 2) the development and validation of an Arab Family Food Security Scale (AFFSS). The surveys used a cluster-randomized sampling design. The 2 food security subscales underwent face and construct validity. In addition, both of these tools and the AFFSS underwent psychometric assessment for internal validity by using statistical methods based on Item Response Theory The food security questions tested by focus groups were understood and accepted in all regions of Lebanon. The food security subscales and the AFFSS had acceptable levels of internal consistency. The psychometric assessment confirmed that the 7 items of the AFFSS had good internal validity and reasonable reliability with item in-fits from 0.73 to 1.16. Food insecurity was identified among 42% of southern Lebanese and 62% of Palestinian refugee households. The determinants and consequences of food security measured in this study provide additional support for the validity of the modules. Using multivariate logistic regression, the higher the mean monthly income per household member and the higher the educational attainment of the head of household, the lower the risk of food insecurity [ORs (95% Cls): 0.99 (0.98, 0.99) and 0.66 (054, 0.80), respectively]. There was a strong significant. association between food insecurity and lower food expenditure and lower intake of all food categories except for legumes, which was significantly associated in the opposite direction (P < 0.001). The odds of borrowing money and accepting gifts/donations were significantly higher among moderately and severely food-insecure households (P< 0.000). The AFFSS has been validated within Lebanon and can potentially be extended to other Arab-speaking populations. C1 [Sahyoun, Nadine R.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Nord, Mark] USDA, Econ Res Serv, Washington, DC USA. [Sassine, Anniebelle J.; Hwalla, Nahla; Ghattas, Hala] Amer Univ Beirut, Dept Nutr & Food Sci, Beirut, Lebanon. [Seyfert, Karin] Univ London, Sch Oriental & African Studies, Dept Econ, London, England. RP Sahyoun, NR (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM nsahyoun@umd.edu RI Nguyen, Giang/D-9027-2016; Ghattas, Hala/K-5661-2016; OI Seyfert, Karin/0000-0002-3354-8844 FU European Union; WHO, Eastern Mediterranean Regional Office (WHO-EMRO) [RPPH 10-53]; Faculty of Agricultural and Food Sciences at the American University of Beirut FX Components of this study were supported by a European Union grant to the United Nations Relief and Works Agency for Palestinian Refugees in the Near East (UNRWA); a grant awarded by the WHO, Eastern Mediterranean Regional Office (WHO-EMRO; RPPH 10-53), and the Faculty of Agricultural and Food Sciences at the American University of Beirut. NR 23 TC 6 Z9 6 U1 3 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 751 EP 757 DI 10.3945/jn.113.187112 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700025 PM 24598883 ER PT J AU Duggan, C Srinivasan, K Thomas, T Samuel, T Rajendran, R Muthayya, S Finkelstein, JL Lukose, A Fawzi, W Allen, LH Bosch, RJ Kurpad, AV AF Duggan, Christopher Srinivasan, Krishnamachari Thomas, Tinku Samuel, Tinu Rajendran, Ramya Muthayya, Sumithra Finkelstein, Julia L. Lukose, Ammu Fawzi, Wafaie Allen, Lindsay H. Bosch, Ronald J. Kurpad, Anura V. TI Vitamin B-12 Supplementation during Pregnancy and Early Lactation Increases Maternal, Breast Milk, and Infant Measures of Vitamin B-12 Statust SO JOURNAL OF NUTRITION LA English DT Article ID MULTIPLE MICRONUTRIENT DEFICIENCIES; TOTAL HOMOCYSTEINE CONCENTRATION; FOLIC-ACID SUPPLEMENTATION; INDIAN WOMEN; COBALAMIN SUPPLEMENTATION; PLASMA HOMOCYSTEINE; METHYLMALONIC ACID; ORAL VITAMIN-B-12; MONTHS POSTPARTUM; HIGH PREVALENCE AB Pregnant women in resource-poor areas are at risk of multiple micronutrient deficiencies, and indicators of low vitamin B-12 status have been associated with adverse pregnancy outcomes, including anemia, low birth weight, and intrauterine growth retardation. To evaluate whether daily oral vitamin B-12 supplementation during pregnancy increases maternal and infant measures of vitamin B-12 status, we performed a randomized, placebo-controlled clinical trial. Pregnant women <14 wk of gestation in Bangalore, India, were randomly assigned to receive daily oral supplementation with vitamin B-12 (50 mu g) or placebo through 6 wk postpartum. All women were administered iron and folic acid supplements throughout pregnancy. One hundred eighty-three women were randomly assigned to receive vitamin B-12 and 183 to receive placebo. Compared with placebo recipients, vitamin B-12 supplemented women had significantly higher plasma vitamin B-12 concentrations at both the second (median vitamin B-12 concentration: 216 vs. 111 pmol/L, P < 0.001) and third (median: 184 vs. 105 pmol/L, P < 0.001) trimesters. At 6 wk postpartum, median breast milk vitamin B-12 concentration was 136 pmol/L in vitamin B-12 supplemented women vs. 87 pmol/L in the placebo group (P < 0.0005). Among vitamin B-12-supplemented women, the Incidence of delivering an infant with intrauterine growth retardation was 33 of 131(25%) vs. 43 of 125 (34%) in those administered placebo (P= 0.11). In a subset of infants tested at 6 wk of age, median plasma vitamin B-12 concentration was 199 pmol/L in those born to supplemented women vs. 139 pmol/L in the placebo group (P = 0.01). Infant plasma methylmalonic acid and homocysteine concentrations were significantly lower in the vitamin B-12 group as well. Oral supplementation of urban Indian women with vitamin B-12 throughout pregnancy and early lactation significantly increases vitamin B-12 status of mothers and infants. It is important to determine whether there are correlations between these findings and neurologic and metabolic functions. C1 [Duggan, Christopher; Samuel, Tinu; Muthayya, Sumithra; Kurpad, Anura V.] St Johns Res Inst, Div Nutr, Bangalore, Karnataka, India. [Srinivasan, Krishnamachari; Rajendran, Ramya; Lukose, Ammu] St Johns Res Inst, Div Mental Hlth & Neurosci, Bangalore, Karnataka, India. [Thomas, Tinku] St Johns Res Inst, Div Epidemiol Biostat & Populat Hlth, Bangalore, Karnataka, India. [Duggan, Christopher; Fawzi, Wafaie] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Fawzi, Wafaie] Harvard Univ, Sch Publ Hlth, Dept Global Hlth & Populat, Boston, MA 02115 USA. [Bosch, Ronald J.] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Finkelstein, Julia L.] Cornell Univ, Div Nutr, Ithaca, NY USA. [Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Duggan, Christopher] Boston Childrens Hosp, Div Gastroenterol Hepatol & Nutr, Boston, MA USA. [Duggan, Christopher] Harvard Univ, Sch Med, Boston, MA USA. RP Duggan, C (reprint author), St Johns Res Inst, Div Nutr, Bangalore, Karnataka, India. EM christopher.duggan@childrens.harvard.edu FU Indian Council of Medical Research grant [5/7/192/06-RHN]; Eunice Kennedy Shriver National Institute of Child Health and Human Development grants [R03 HD054123, K24HD058795] FX Supported by Indian Council of Medical Research grant 5/7/192/06-RHN and Eunice Kennedy Shriver National Institute of Child Health and Human Development grants R03 HD054123 and K24HD058795. NR 43 TC 20 Z9 21 U1 3 U2 13 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 758 EP 764 DI 10.3945/jn.113.187278 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700026 PM 24598885 ER PT J AU Wong, WW Roberts, SB Racette, SB Das, SK Redman, LM Rochon, J Bhapkar, MV Clarke, LL Kraus, WE AF Wong, William W. Roberts, Susan B. Racette, Susan B. Das, Sai Krupa Redman, Leanne M. Rochon, James Bhapkar, Manjushri V. Clarke, Lucinda L. Kraus, William E. TI The Doubly Labeled Water Method Produces Highly Reproducible Longitudinal Results in Nutrition Studies SO JOURNAL OF NUTRITION LA English DT Article ID RING-DOWN SPECTROSCOPY; MEASURING ENERGY-EXPENDITURE; ISOTOPE RATIO MEASUREMENTS; BODY-COMPOSITION; INDIRECT-CALORIMETRY; PHYSICAL-ACTIVITY; MATERIAL BALANCE; CO2 OUTPUT; COMPREHENSIVE ASSESSMENT; LACTATION PERFORMANCE AB The doubly labeled water (DLW) method is considered the reference method for the measurement of energy expenditure under free-living conditions. However, the reproducibility of the DLW method in longitudinal studies is not well documented. This study was designed to evaluate the longitudinal reproducibility of the DLW method using 2 protocols developed and implemented in a multicenter clinical trial the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy (CALERIE). To document the longitudinal reproducibility of the DLW method, 2 protocols, 1 based on repeated analysis of dose dilutions over the course of the clinical trial (dose-dilution protocol) and 1 based on repeated but blinded analysis of randomly selected DLW studies (test-retest protocol), were carried out. The dose-dilution protocol showed that the theoretical fractional turnover rates for H-2 and O-18 and the difference between the 2 fractional turnover rates were reproducible to within 1% and 5%, respectively, over 4.5 y. The Bland-Altman pair-wise comparisons of the results generated from 50 test-retest DLW studies showed that the fractional turnover rates and isotope dilution spaces for H-2 and O-18, and total energy expenditure, were highly reproducible over 2.4 y. Our results show that the DLW method is reproducible in longitudinal studies and confirm the validity of this method to measure energy expenditure, define energy intake prescriptions, and monitor adherence and body composition changes over the period of 2.5-4.4 y. The 2 protocols can be adopted by other laboratories to document the longitudinal reproducibility of their measurements to ensure the long-term outcomes of interest are meaningful biologically. C1 [Wong, William W.] ARS, USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. [Roberts, Susan B.; Clarke, Lucinda L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Racette, Susan B.] Washington Univ, Sch Med, St Louis, MO USA. [Das, Sai Krupa; Redman, Leanne M.] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Rochon, James; Bhapkar, Manjushri V.; Kraus, William E.] Duke Univ, Duke Clin Res Inst, Durham, NC USA. RP Wong, WW (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. EM wwong@bcm.edu OI Racette, Susan/0000-0002-6932-1887 FU National Institute on Aging [5U01-AG-022132-05]; USDA/Agricultural Research Service [6250-51000-053] FX Funded by the National Institute on Aging grant 5U01-AG-022132-05 with support from the USDA/Agricultural Research Service grant 6250-51000-053. The contents of this publication do not necessarily reflect the views or policies of the USDA or the NIH, nor does mention of trade names, commercial products, or organizations imply endorsement. NR 62 TC 7 Z9 7 U1 0 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2014 VL 144 IS 5 BP 777 EP 783 DI 10.3945/jn.113.187823 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8MQ UT WOS:000334970700029 PM 24523488 ER PT J AU Haven, J Maniscalco, S Bard, S Ciampo, M AF Haven, Jackie Maniscalco, Shelley Bard, Sasha Ciampo, Melissa TI MyPlate Myths Debunked SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Haven, Jackie; Maniscalco, Shelley] USDA, Off Nutr Mkt & Commun, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. [Bard, Sasha; Ciampo, Melissa] Panum Grp, Bethesda, MD USA. RP Haven, J (reprint author), USDA, Off Nutr Mkt & Commun, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 1 TC 3 Z9 3 U1 0 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAY PY 2014 VL 114 IS 5 BP 674 EP 675 DI 10.1016/j.jand.2014.03.006 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AG4XR UT WOS:000335424200003 PM 24731523 ER PT J AU Economos, CD Moore, CE Hyatt, RR Kuder, J Chen, T Meydani, SN Meydani, M Klein, E Biancuzzo, RM Holick, MF AF Economos, Christina D. Moore, Carolyn E. Hyatt, Raymond R. Kuder, Julia Chen, Tai Meydani, Simin Nikbin Meydani, Mohsen Klein, Ellen Biancuzzo, Rachael M. Holick, Michael F. TI Multinutrient-Fortified Juices Improve Vitamin D and Vitamin E Status in Children: A Randomized Controlled Trial SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Vitamin D; Vitamin E; Multinutrient juice ID NUTRITION EXAMINATION SURVEY; D DEFICIENCY; ALPHA-TOCOPHEROL; NATIONAL-HEALTH; US POPULATION; UNITED-STATES; ADOLESCENTS; RICKETS; RETINOL; WOMEN AB Background Provision of fortified juices. may provide a convenient method.to maintain and increase blood fat-soluble vitamins. Objective To determine whether children consuming orange juice fortified with calcium and combinations of vitamins D, E, and A could increase serum 25-hydroxyvitamin D [25(OH)D], alpha-tocopherol, and retinol levels. Design A 12-week randomized, double-blind, controlled trial. Participants/setting One hundred eighty participants (aged 8.04 +/- 1.42 years) were recruited at Tufts (n=70) and Boston University (n=110) during 2005-2006. Of those recruited, 176 children were randomized into three groups: CaD (700 mg calcium+200 IU vitamin D), CaDEA (700 mg calcium+200 IU vitamin D+12 IU vitamin E+2,000 IU vitamin A as beta carotene), or Ca (700 mg calcium). Children consumed two 240-mL glasses of CaD, CaDEA, or Ca fortified orange juice daily for 12 weeks. Main outcome measures Serum 25(OH)D, alpha-tocopherol, and retinol concentrations. Statistical analyses Changes in 25(OH)D, alpha-tocopherol, retinol, and parathyroid hormone concentrations were examined. Covariates included sex, age, race/ethnicity, body mass index, and baseline 25(OH)D, alpha-tocopherol, retinol, or parathyroid hormone levels. Multivariate models and repeated measures analysis of variance tested for group differences with pre-post measures (n=141). Results Baseline 25(OH)D was 68.4 +/- 27.7 nmol/L (27.4 +/- 11.10 ng/mL), with 21.7% of participants having inadequate 25(OH)D (< 50 nmol/L [20.03 ng/mL]). The CaD group's 25(OH)D increase was greater than that of the Ca group (12.7 nmol/L [5.09 ng/mL], 95% CI 1.3 to 24.1; P=0.029). The CaDEA group's increase in a-tocopherol concentration was greater than that in the Ca or CaD groups (3.79,mu mol/L 10.16 mu g/mL), 95% CI 2.5 to 5.1 and 3.09 mu mol/L ([0.13 mu g/mL], 95% Cl 1.8 to 4.3), respectively (P < 0.0001). Retinol levels did not change, and body weight remained as expected for growth. Conclusions Daily consumption of orange juice providing 200 IU vitamin D and 12 IU vitamin E increased 25(OH)D and alpha-tocopherol concentrations in young children within 12 weeks. C1 [Economos, Christina D.; Meydani, Simin Nikbin] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Moore, Carolyn E.] Texas Womans Univ, Houston, TX USA. [Hyatt, Raymond R.] Tufts Univ, Boston, MA 02111 USA. [Kuder, Julia] Brigham & Womens Hosp, TIMI Study Grp, Boston, MA 02115 USA. [Chen, Tai; Klein, Ellen; Biancuzzo, Rachael M.; Holick, Michael F.] Boston Univ, Sch Med, Boston, MA 02118 USA. [Meydani, Simin Nikbin; Meydani, Mohsen] Tufts Univ, Jean Mayer USDA HNRCA, Boston, MA 02111 USA. [Meydani, Simin Nikbin] Tufts Univ, Friedman Sch Nutr Sci & Policy, Nutr Immunol Lab, Boston, MA 02111 USA. [Meydani, Simin Nikbin] Tufts Univ, Sackler Grad Sch, Boston, MA 02111 USA. RP Economos, CD (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, 150 Harrison Ave, Boston, MA 02111 USA. EM christina.economos@tufts.edu FU Beverage Institute for Health Wellness; Coca Cola Company, Atlanta, GA FX This research was sponsored by The Beverage Institute for Health & Wellness, The Coca Cola Company, Atlanta, GA. The sponsor had no role in study design; the collection, analysis, and interpretation of data; the writing of the report; or the decision to submit the report for publication. Also, no honorarium, grant, or other form of payment was given to anyone to produce the manuscript. NR 35 TC 7 Z9 8 U1 1 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAY PY 2014 VL 114 IS 5 BP 709 EP 717 DI 10.1016/j.jand.2013.07.027 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AG4XR UT WOS:000335424200008 PM 24139824 ER PT J AU Dickson, BG Sisk, TD Sesnie, SE Reynolds, RT Rosenstock, SS Vojta, CD Ingraldi, MF Rundall, JM AF Dickson, Brett G. Sisk, Thomas D. Sesnie, Steven E. Reynolds, Richard T. Rosenstock, Steven S. Vojta, Christina D. Ingraldi, Michael F. Rundall, Jill M. TI Integrating single-species management and landscape conservation using regional habitat occurrence models: the northern goshawk in the Southwest, USA SO LANDSCAPE ECOLOGY LA English DT Article DE Accipiter gentilis; Forest management; Multi-model inference; Pinus ponderosa; Restoration; Spatial model ID WESTERN UNITED-STATES; NESTING HABITAT; FOREST STRUCTURE; ROCKY-MOUNTAINS; SELECTION; RESTORATION; POPULATIONS; PERSPECTIVE; ECOSYSTEMS; OCCUPANCY AB Conservation planners and land managers are often confronted with scale-associated challenges when assessing the relationship between land management objectives and species conservation. Conservation of individual species typically involves site-level analyses of habitat, whereas land management focuses on larger spatial extents. New models are needed to more explicitly integrate species-specific conservation with landscape or regional scales. We address this challenge with an example using the northern goshawk (Accipiter gentilis), a forest raptor with circumpolar distribution that is the focus of intense debate regarding forest management on public lands in the southwestern USA. To address goshawk-specific habitat conservation across a management area of 22,800-km(2) in northern Arizona, we focused on the territory scale rather than individual nest sites. We compiled a 17-year database of 895 nest sites to estimate territory locations. We then estimated the likelihood of territory occurrence for the entire management area using multiple logistic regression within an expert-driven, spatially balanced, and information-theoretic framework. Our occurrence model incorporated forest structure variables that were derived from USFS Forest Inventory and Analysis plots and high-resolution satellite imagery. Results indicated that high canopy-bulk density, intermediate canopy-base heights, and low variation in tree density were strong predictors of territory occurrence. We used model-averaged parameter estimates for these variables to map and explore patterns of territory distribution across multiple land jurisdictions and ecological subregions. Our iterative modeling approach complements previous demographic studies in the region. It also provides a robust framework for integrating species conservation and landscape management in ongoing and future regional planning efforts. C1 [Dickson, Brett G.; Sisk, Thomas D.; Sesnie, Steven E.; Vojta, Christina D.; Rundall, Jill M.] No Arizona Univ, Lab Landscape Ecol & Conservat Biol, Landscape Conservat Initiat, Flagstaff, AZ 86011 USA. [Dickson, Brett G.] Conservat Sci Partners Inc, Truckee, CA 96161 USA. [Reynolds, Richard T.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Rosenstock, Steven S.; Ingraldi, Michael F.] Arizona Game & Fish Dept, Res Branch, Phoenix, AZ 85086 USA. RP Dickson, BG (reprint author), No Arizona Univ, Lab Landscape Ecol & Conservat Biol, Landscape Conservat Initiat, POB 5694, Flagstaff, AZ 86011 USA. EM brett.dickson@nau.edu FU U.S. Forest Service; Northern Arizona University Ecological Restoration Institute; Grand Canyon Trust; Northern Arizona University FX We thank biologists with the Kaibab, Coconino, Apache-Sitgreaves, and Tonto National Forests for providing historical goshawk location data. Funding was provided by the U.S. Forest Service, the Northern Arizona University Ecological Restoration Institute, The Grand Canyon Trust, and Northern Arizona University. C. Ray and two anonymous reviewers provided valuable comments on earlier drafts of this work. NR 48 TC 4 Z9 4 U1 6 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAY PY 2014 VL 29 IS 5 BP 803 EP 815 DI 10.1007/s10980-014-0013-3 PG 13 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AF4NL UT WOS:000334689900004 ER PT J AU Hirahatake, KM Slavin, JL Maki, KC Adams, SH AF Hirahatake, Kristin M. Slavin, Joanne L. Maki, Kevin C. Adams, Sean H. TI Associations between dairy foods, diabetes, and metabolic health: Potential mechanisms and future directions SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article DE Type 2 diabetes mellitus; Metabolic syndrome; Prediabetes; Dietary Guidelines for Americans ID GENE-RELATED PEPTIDE; TRANS-PALMITOLEIC ACID; MESSENGER-RNA LEVELS; HIGH-CALCIUM DIET; SKELETAL-MUSCLE; ADIPOSE-TISSUE; WHEY-PROTEIN; RISK-FACTORS; INFLAMMATORY RESPONSES; INSULIN SENSITIVITY AB Epidemiological evidence supports an inverse relationship between adequate intake of dairy foods and susceptibility to type 2 diabetes (T2D). The biological mechanisms responsible for this association remain to be established. This review provides a current perspective on proposed mechanisms that may underlie these effects, and highlights how randomized clinical trials can be applied to investigate these relationships. Results from epidemiological studies generally support that consumption of milk and dairy products is associated with a lower incidence of T2D or improvements in glucose homeostasis indices, and studies of animal and cell models support a positive effect of dairy-rich diets or components on metabolic and inflammation factors relevant to T2D and insulin resistance. Emerging evidence indicates that dairy components that alter mitochondrial function (e.g., leucine actions on silent information regulator transcript 1 (SIRT1)-associated pathways), promote gut microbial population shifts, or influence inflammation and cardiovascular function (e.g., Ca-regulated peptides calcitonin gene-related peptide [CGRP] or calcitonin) should be considered as possible mechanistic factors linking dairy intake with lower risk for T2D. The possibility that dairy-derived trans-palmitoleic acid (tC16:1) has metabolic bioactivities has also been proposed. Pre-clinical and clinical studies focusing specifically on these parameters are needed to validate hypotheses regarding the potential roles of dairy products and their components on the determinants of glucose tolerance, particularly insulin sensitivity, pancreatic endocrine function, and inflammation in individuals at-risk for T2D development. Such experiments would complement epidemiological studies and add to the evidence base for recommendations regarding consumption of dairy products and their individual components. Published by Elsevier Inc. C1 [Hirahatake, Kristin M.; Adams, Sean H.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Slavin, Joanne L.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN USA. [Maki, Kevin C.] Biofortis Clin Res, Addison, IL USA. [Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. RP Adams, SH (reprint author), USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM sean.h.adams@ars.usda.gov FU USDA-ARS [5306-51530-019-00]; NIH-NIDDK [R01DK078328]; Dairy Research Institute FX Authors acknowledge support from USDA-ARS intramural Project 5306-51530-019-00 (SHA), NIH-NIDDK R01DK078328 (SHA), and research grants from the Dairy Research Institute (SHA). USDA is an equal opportunity provider and employer. KCM has received research grant support as well as honoraria for speaking at events sponsored by Dairy Research Institute. NR 111 TC 24 Z9 26 U1 3 U2 54 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 EI 1532-8600 J9 METABOLISM JI Metab.-Clin. Exp. PD MAY PY 2014 VL 63 IS 5 BP 618 EP 627 DI 10.1016/j.metabol.2014.02.009 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AG1UC UT WOS:000335200700003 PM 24636056 ER PT J AU Sullivan, ML AF Sullivan, Michael L. TI Perennial peanut (Arachis glabrata Benth.) leaves contain hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase activity and accumulate hydroxycinnamoyl-tartaric acid esters SO PLANTA LA English DT Article DE BAHD acyl transferase; Caftaric acid; Hydroxycinnamoyl-CoA:shikimic acid hydroxycinnamoyl transferase; Hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase; Hydroxycinnamoyl-CoA thiolesterase ID LIGASE GENE FAMILY; POLYPHENOL OXIDASE; RED-CLOVER; 4-COUMARATE-COA LIGASE; POSTHARVEST PROTEOLYSIS; RAPHANUS-SATIVUS; CHLOROGENIC ACID; DOWN-REGULATION; PHASELIC ACID; BIOSYNTHESIS AB Many plants accumulate hydroxycinnamoyl esters to protect against abiotic and biotic stresses. Caffeoyl esters in particular can be substrates for endogenous polyphenol oxidases (PPOs). Recently, we showed that perennial peanut (Arachis glabrata Benth.) leaves contain PPO and identified one PPO substrate, caftaric acid (trans-caffeoyl-tartaric acid). Additional compounds were believed to be cis- and trans-p-coumaroyl tartaric acid and cis- and trans-feruloyl-tartaric acid, but lack of standards prevented definitive identifications. Here we characterize enzymatic activities in peanut leaves to understand how caftaric acid and related hydroxycinnamoyl esters are made in this species. We show that peanut leaves contain a hydroxycinnamoyl-CoA:tartaric acid hydroxycinnamoyl transferase (HTT) activity capable of transferring p-coumaroyl, caffeoyl, and feruloyl moieties from CoA to tartaric acid (specific activities of 11 +/- A 2.8, 8 +/- A 1.8, 4 +/- A 0.8 pkat mg(-1) crude protein, respectively). The HTT activity was used to make cis- and trans-p-coumaroyl- and -feruloyl-tartaric acid in vitro. These products allowed definitive identification of the corresponding cis- and trans-hydroxycinnamoyl esters extracted from leaves. We tentatively identified sinapoyl-tartaric acid as another major phenolic compound in peanut leaves that likely participates in secondary reactions with PPO-generated quinones. These results suggest hydroxycinnamoyl-tartaric acid esters are made by an acyltransferase, possibly a BAHD family member, in perennial peanut. Identification of a gene encoding HTT and further characterization of the enzyme will aid in identifying determinants of donor and acceptor substrate specificity for this important class of biosynthetic enzymes. An HTT gene could also provide a means by genetic engineering for producing caffeoyl- and other hydroxycinnamoyl-tartaric acid esters in forage crops that lack them. C1 ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53705 USA. RP Sullivan, ML (reprint author), ARS, US Dairy Forage Res Ctr, USDA, 1925 Linden Dr, Madison, WI 53705 USA. EM michael.sullivan@ars.usda.gov NR 36 TC 6 Z9 7 U1 1 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD MAY PY 2014 VL 239 IS 5 BP 1091 EP 1100 DI 10.1007/s00425-014-2038-x PG 10 WC Plant Sciences SC Plant Sciences GA AG1EU UT WOS:000335158700013 PM 24556732 ER PT J AU Roemmich, JN Beeler, JE Johnson, L AF Roemmich, James N. Beeler, Joley E. Johnson, LuAnn TI A microenvironment approach to reducing sedentary time and increasing physical activity of children and adults at a playground SO PREVENTIVE MEDICINE LA English DT Article DE Parks; Playgrounds; Physical activity; Youth; Children; Micro-environment; Built environment; Social facilitation ID BUILT ENVIRONMENT; OBESOGENIC ENVIRONMENTS; CARDIOVASCULAR-DISEASE; NATURAL EXPERIMENT; ACTIVITY BEHAVIORS; PARK IMPROVEMENTS; SITTING TIME; OBESITY; HEALTH; ASSOCIATION AB Objective. Test whether a micro-environment park intervention in Grand Forks, North Dakota, movement of seating away from a playground, would increase the physical activity and length of stay of park users. Method. Study 1, summer 2012: physical activity of children and adults was assessed during baseline (A(1)) with seating in usual, standardized locations; with seating removed from the playground (B); and with seating returned to original locations (A(2)). Study 2, summer 2013: the study was repeated with the inclusion of a daily 2-hour assessment during which activity of each family member was recorded every 15-min and length of stay was recorded. Results. For both studies, the MET (metabolic equivalent) intensity was greater (p < 0.02) during condition B than during A(1) and Ay. For adults, the odds of being in moderate-to-vigorous physical activity than sitting during condition B were 4.1 to 22.7 greater than those during conditions A1 and A(2). During the 2-hour serial observations, MET intensities during condition B were greater (p < 0.005) than those during A(1) and A(2). The duration families stayed at the park did not differ across conditions. Conclusion. Adults were more active when seating was not accessible. Removal of seating did not shorten the time that adults were willing to allow children to play. Published by Elsevier Inc. C1 [Roemmich, James N.; Beeler, Joley E.; Johnson, LuAnn] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. RP Roemmich, JN (reprint author), ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. EM james.roemmich@ars.usda.gov FU USDA/Agricultural Research Service [USDA 5450-51000-049-00D] FX We thank the Grand Forks Park District for manipulating access to picnic tables by removing them from the playground area and adjacent shelters for the duration of the B condition. This research was supported by the USDA/Agricultural Research Service, USDA 5450-51000-049-00D. The contents of this publication do not necessarily reflect the views or policies of the USDA or the Agricultural Research Service, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. The study sponsor had no involvement in the study design; collection, analysis and interpretation of data; the writing of the manuscript; and the decision to submit the manuscript for publication. NR 46 TC 4 Z9 4 U1 2 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0091-7435 EI 1096-0260 J9 PREV MED JI Prev. Med. PD MAY PY 2014 VL 62 BP 108 EP 112 DI 10.1016/j.ypmed.2014.01.018 PG 5 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA AF7MS UT WOS:000334899800020 PM 24502848 ER PT J AU Green, CO Badaloo, AV Hsu, JW Taylor-Bryan, C Reid, M Forrester, T Jahoor, F AF Green, Curtis O. Badaloo, Asha V. Hsu, Jean W. Taylor-Bryan, Carolyn Reid, Marvin Forrester, Terrence Jahoor, Farook TI Effects of randomized supplementation of methionine or alanine on cysteine and glutathione production during the early phase of treatment of children with edematous malnutrition SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID SEVERE CHILDHOOD UNDERNUTRITION; AMINO-ACID-METABOLISM; ERYTHROCYTE GLUTATHIONE; N-ACETYLCYSTEINE; KWASHIORKOR; PROTEIN; PATHOGENESIS; KINETICS; HUMANS; BLOOD AB Background: We have shown that a low glutathione concentration and synthesis rate in erythrocytes are associated with a shortage of protein-derived cysteine in children with edematous severe acute malnutrition (SAM). Objective: We tested the hypothesis that methionine supplementation may increase protein-derived cysteine and upregulate cysteine synthesis, thereby improving glutathione synthesis during the early treatment of edematous SAM. Design: The cysteine flux, its de novo synthesis and release from protein breakdown, and erythrocyte glutathione synthesis rate were measured in 12 children with edematous SAM in the fed state by using stable isotope tracers at 3 clinical phases as follows: 3 +/- 1 d (+/- SE) [clinical phase 1 (CP1)], 8 +/- 1 d [clinical phase 2 (CP2)], and 14 +/- 2 d (clinical phase 3) after admission. Subjects were randomly assigned to receive equimolar supplements (0.5 mmol . kg(-1) . d(-1)) of methionine or alanine (control) immediately after CPI. Results: In the methionine compared with the alanine group, cysteine flux derived from protein breakdown was faster at CP2 than CP1 (P < 0.05), and the change in plasma cysteine concentration from CPI to CP2 was greater (P < 0.05). However, there was no evidence of a difference in cysteine de novo synthesis and its total flux or erythrocyte glutathione synthesis rate and concentration between groups. Conclusions: Methionine supplementation increased cysteine flux from body protein but had no significant effect on glutathione synthesis rates. Although cysteine is made from methionine, increased dietary cysteine may be necessary to partially fulfill its demand in edematous SAM because glutathione synthesis rates and concentrations were less than previous values shown at full recovery. This study was registered at clinicaltrials.gov as NCT00473031. C1 [Green, Curtis O.; Badaloo, Asha V.; Taylor-Bryan, Carolyn; Reid, Marvin; Forrester, Terrence] Univ W Indies, Trop Metab Res Unit, Res Inst Trop Med, Kingston 7, Jamaica. [Hsu, Jean W.; Jahoor, Farook] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Badaloo, AV (reprint author), Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. EM asha.badaloo@uwimona.edu.jm OI Reid, Marvin/0000-0003-4005-9384 FU NIH [2RO1 DK 056689]; USDA, Agricultural Research Service [58-6250-6001] FX Supported by the NIH (grant 2RO1 DK 056689) and federal funds from the USDA, Agricultural Research Service (cooperative agreement 58-6250-6001). NR 24 TC 1 Z9 1 U1 1 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2014 VL 99 IS 5 BP 1052 EP 1058 DI 10.3945/ajcn.113.062729 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF7LD UT WOS:000334895700013 PM 24598154 ER PT J AU Jacques, PF Wang, HF AF Jacques, Paul F. Wang, Huifen TI Yogurt and weight management SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID DAIRY CONSUMPTION; BODY-WEIGHT; INTESTINAL MICROBIOTA; WAIST CIRCUMFERENCE; MILK-PRODUCTS; FAT LOSS; OBESITY; ADULTS; DIET; SATIETY AB A large body of observational studies and randomized controlled trials (RCTs) has examined the role of dairy products in weight loss and maintenance of healthy weight. Yogurt is a dairy product that is generally very similar to milk, but it also has some unique properties that may enhance its possible role in weight maintenance. This review summarizes the human RCT and prospective observational evidence on the relation of yogurt consumption to the management and maintenance of body weight and Composition. The RCT evidence is limited to 2 small, short-term, energy-restricted trials. They both showed greater weight losses with yogurt: interventions, but the difference between the yogurt intervention and the control diet was only significant in one of these trials. There are 5 prospective observational studies that have examined the association between yogurt and weight gain. The results of these studies are equivocal. Two of these studies reported that individuals with higher yogurt consumption gained less weight over time. One of these same studies also considered changes in waist circumference (WC) and showed that higher yogurt consumption was associated with smaller increases in WC. A third study was inconclusive because of low statistical power. A fourth study observed no association between changes in yogurt intake and weight gain, but the results suggested that those with the largest increases in yogurt intake during the study also had the highest increase in WC. The final study examined weight and WC change separately by sex and baseline weight status and showed benefits for both weight and WC changes for higher yogurt consumption in overweight men, but it also found that higher yogurt consumption in normal-weight women was associated with a greater increase in weight over follow-up. Potential underlying mechanisms for the action of yogurt on weight are briefly discussed. C1 [Jacques, Paul F.; Wang, Huifen] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Jacques, Paul F.] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. RP Jacques, PF (reprint author), 711 Washington St, Boston, MA 02111 USA. EM paul.jacques@tufts.edu FU USDA [58-1950-0-014]; Dannon Company Inc. FX Supported by USDA Agreement 58-1950-0-014 and a research grant from The Dannon Company Inc. NR 41 TC 4 Z9 4 U1 2 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2014 VL 99 IS 5 BP 1229S EP 1234S DI 10.3945/ajcn.113.073031 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF7LD UT WOS:000334895700039 PM 24695887 ER PT J AU El-Abbadi, NH Dao, MC Meydani, SN AF El-Abbadi, Nagac Hani Dao, Maria Carlota Meydani, Simin Nikbin TI Yogurt: role in healthy and active aging SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; VITAMIN-D; POSTMENOPAUSAL WOMEN; BONE-RESORPTION; DAIRY-PRODUCTS; IMMUNE-SYSTEM; OLDER-ADULTS; DOUBLE-BLIND; CONSUMPTION; RISK AB Yogurt consumption has been associated with health benefits in different populations. Limited information, however, is available on nutritional and health attributes of yogurt in older adults. Yogurt is abundant in calcium, zinc. B. vitamins, and probiotics; it is a good source of protein; and it may be supplemented with vitamin D and additional probiotics associated with positive health outcomes. Aging is accompanied by a wide array of nutritional deficiencies and health complications associated with under- and overnutrition, including musculoskeletal impairment, immunosenescence, cardiometabolic diseases, and cognitive impairment. Furthermore, yogurt is accessible and convenient to consume by the older population, which makes yogurt consumption a feasible approach to enhance older adults' nutritional status. A limited number of studies have specifically addressed the impact of yogurt on the nutritional and health status of older adults, and most are observational. However, those reported thus far and reviewed here are encouraging and suggest that yogurt could play a role in improving the nutritional status and health of older adults. In addition, these reports support further investigation into the role of yogurt in healthy and active aging. C1 [El-Abbadi, Nagac Hani; Dao, Maria Carlota; Meydani, Simin Nikbin] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Meydani, SN (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM simin.meydani@tufts.edu FU NHE-A; MCD; SNM; USDA [58-1950-0-014] FX Sources of grant support for NHE-A, MCD, and SNM include USDA agreements 58-1950-0-014 NR 54 TC 12 Z9 13 U1 7 U2 34 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2014 VL 99 IS 5 BP 1263S EP 1270S DI 10.3945/ajcn.113.073957 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF7LD UT WOS:000334895700045 PM 24695886 ER PT J AU Lowry, DB Behrman, KD Grabowski, P Morris, GP Kiniry, JR Juenger, TE AF Lowry, David B. Behrman, Kathrine D. Grabowski, Paul Morris, Geoffrey P. Kiniry, James R. Juenger, Thomas E. TI Adaptations between Ecotypes and along Environmental Gradients in Panicum virgatum* SO AMERICAN NATURALIST LA English DT Article DE population structure; switchgrass; cline; ecotype; reciprocal transplant; local adaptation ID GENOME-WIDE ASSOCIATION; TRANSPLANTED COMMUNITY FRACTIONS; LOW-TEMPERATURE TOLERANCE; AMERICAN PRAIRIE GRASSES; CENTRAL US ENVIRONMENTS; PLANT COLD-ACCLIMATION; CENTRAL NORTH-AMERICA; NUCLEAR-DNA CONTENT; LOCAL ADAPTATION; SWITCHGRASS POPULATIONS AB Determining the patterns and mechanisms of natural selection in the wild is of fundamental importance to understanding the differentiation of populations and the evolution of new species. However, it is often unknown the extent to which adaptive genetic variation is distributed among ecotypes between distinct habitats versus along large-scale geographic environmental gradients, such as those that track latitude. Classic studies of selection in the wild in switchgrass, Panicum virgatum, tested for adaptation at both of these levels of natural variation. Here we review what these field experiments and modern agronomic field trials have taught us about natural variation and selection at both the ecotype and environmental gradient levels in P. virgatum. With recent genome sequencing efforts in P. virgatum, it is poised to become an excellent system for understanding the adaptation of grassland species across the eastern half of North America. The identification of genetic loci involved in different types of adaptations will help to understand the evolutionary mechanisms of diversification within P. virgatum and provide useful information for the breeding of high-yielding cultivars for different ecoregions. C1 [Lowry, David B.; Juenger, Thomas E.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [Behrman, Kathrine D.; Kiniry, James R.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Grabowski, Paul] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA. [Morris, Geoffrey P.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. RP Lowry, DB (reprint author), Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. EM davidbryantlowry@gmail.com OI Morris, Geoffrey/0000-0002-3067-3359 FU National Science Foundation Plant Genome Research Program award [IOS-0922457]; USDA National Institute of Food and Agriculture-Agriculture and Food Research Initiative postdoctoral fellowship [2011-67012-30696] FX We would like to thank H. E. Hoekstra and two anonymous reviewers for comments that helped to improve the manuscript. A. Asmus, D. Dillon, and T. Logan assisted with flow cytometry and chloroplast genotyping. This project was funded by a National Science Foundation Plant Genome Research Program award (IOS-0922457) to T.E.J. and a USDA National Institute of Food and Agriculture-Agriculture and Food Research Initiative postdoctoral fellowship (2011-67012-30696) to D. B. L. The USDA is an equal-opportunity provider and employer. NR 143 TC 18 Z9 18 U1 10 U2 80 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 0003-0147 EI 1537-5323 J9 AM NAT JI Am. Nat. PD MAY 1 PY 2014 VL 183 IS 5 BP 682 EP 692 DI 10.1086/675760 PG 11 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA AE9MR UT WOS:000334332400012 PM 24739200 ER PT J AU Scholljegerdes, EJ Lekatz, LA Vonnahme, KA AF Scholljegerdes, E. J. Lekatz, L. A. Vonnahme, K. A. TI Effects of short-term oilseed supplementation on plasma fatty acid composition, progesterone and prostaglandin F metabolite in lactating beef cows SO ANIMAL LA English DT Article DE soybean; prostaglandin metabolite; flaxseed; fatty acid; cow ID DAIRY-COWS; RUMINAL BIOHYDROGENATION; EMBRYO SURVIVAL; FORAGE INTAKE; HEIFERS; EWES; PERFORMANCE; PREGNANCY; CATTLE; REPRODUCTION AB Twenty-four 3-year-old Angus cows (512.2 +/- 21.6 kg) and six ruminally cannulated beef heifers (523.1 +/- 16.9 kg) were used to determine the impact of feeding oilseeds starting at the beginning of estrous synchronization until maternal recognition of pregnancy on plasma fatty acid composition. Starting 60 days postpartum cows were synchronized with the Select Synch + controlled internal drug-release (CIDR) device and timed artificial insemination (AI) protocol. The day CIDR was inserted; cattle were randomly assigned to one of the three treatments being grazing only (CON) or a supplement containing whole soybeans (SOY); or whole flaxseed (FLX). Cattle continued to receive these diets for 28 days. Blood was collected every 3 days until 10 days after insemination and then every day until 18 days after insemination. All cattle grazed a common pasture and supplemented cattle were individually fed their respective supplements once daily. Ruminally cannulated heifers were used to evaluate the impact supplements had on forage intake, which was reduced (P = 0.05) with oilseed supplementation. Feeding oilseeds increased total fatty acid intake (P < 0.001) across treatments with SOY having greater (P < 0.001) 18:2n-6 intake than either CON or FLX. Likewise, cattle fed FLX had greater (P < 0.001) 18:3n-3 intake than either CON or SOY. There was a treatmentxtime interaction (P <= 0.05) for all fatty acids identified except for 20:5n-3 (P = 0.99). Within 3 days after the start of supplementation, plasma concentrations of 18:2n-6 increased (P < 0.001) for cattle fed SOY compared with CON or FLX, whereas flax-fed cattle did not exhibit an increase (P = 0.02) until day 15 of supplementation over that of CON. Plasma concentrations for 18:3n-3 was greater (P < 0.013) for FLX than both CON and SOY by day 12. Feeding flaxseed tended to (P = 0.07) increase and increased (P = 0.01) plasma concentrations of 20:4n-6 by day 18 over CON and SOY, respectively. Overall, treatment did not affect serum concentration of progesterone (P = 0.18) or prostaglandin F metabolite (P = 0.89). However, day after breeding had an effect on serum progesterone (P = 0.01) with day 16 after timed AI being lower compared with other days. Feeding oilseeds during the time of estrous synchronization will not only increase the energy density of the diet but will provide key fatty acids around the time of maternal recognition of pregnancy. C1 [Scholljegerdes, E. J.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Lekatz, L. A.] Illinois State Univ, Dept Agr, Normal, IL 61761 USA. [Vonnahme, K. A.] N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58102 USA. RP Scholljegerdes, EJ (reprint author), New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. EM ejs@nmsu.edu NR 38 TC 2 Z9 2 U1 2 U2 10 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD MAY PY 2014 VL 8 IS 5 BP 777 EP 785 DI 10.1017/S1751731114000263 PG 9 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AF3CF UT WOS:000334588400012 PM 24572170 ER PT J AU Frankel, LA O'Connor, TM Chen, TA Nicklas, T Power, TG Hughes, SO AF Frankel, Leslie A. O'Connor, Teresia M. Chen, Tzu-An Nicklas, Theresa Power, Thomas G. Hughes, Sheryl O. TI Parents' perceptions of preschool children's ability to regulate eating. Feeding style differences SO APPETITE LA English DT Article DE Self-regulation; Feeding styles; Weight status; Preschoolers; Parental feeding ID INCREASES ENERGY-INTAKE; PORTION SIZE; AMERICAN CHILDREN; SELF-REGULATION; FOOD-INTAKE; BEHAVIORAL SUSCEPTIBILITY; CALORIC COMPENSATION; AFRICAN-AMERICAN; YOUNG-CHILDREN; WEIGHT STATUS AB Parent feeding styles have been associated with children's eating behaviors and weight status across multiple studies. However, little is known about the mechanism through which parent feeding styles influence child weight status. Children's ability to self-regulate their eating may be the mechanism that links these two constructs. This study examined the relationship between parent feeding styles, child self-regulatory ability, and weight status to determine whether self-regulatory ability mediated the relationship between parent feeding styles and child weight status in a group of 296 parents and their preschool aged children. Indulgent feeding style was related to children having lessened satiety responsiveness and higher enjoyment of food (two components of self-regulation around eating) compared to other parent feeding styles. Children of parents with an indulgent feeding style were also higher in weight status compared to other feeding styles. Mediation analyses revealed that satiety responsiveness and enjoyment of food mediated the relationship between parent feeding style and child BMI z-score such that children of parents with indulgent feeding styles had lessened ability to self-regulate around eating and higher BMI z-scores. Findings from this study suggest that children's ability to self-regulate eating might be an important mechanism by which parent feeding style and child weight are related to each other. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Frankel, Leslie A.; O'Connor, Teresia M.; Chen, Tzu-An; Nicklas, Theresa; Hughes, Sheryl O.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Power, Thomas G.] Washington State Univ, Dept Human Dev, Pullman, WA 99164 USA. [Frankel, Leslie A.] Univ Houston, Dept Educ Psychol, Houston, TX 77004 USA. RP Hughes, SO (reprint author), Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM Shughes@bcm.edu FU USDA/ARS Children's Nutrition Research [6250-51000]; United States Department of Agriculture,and, in part, with funds from Kraft, Inc [2006-55215-16695]; USDA/ARS [6250-51000-053] FX The first author conducted these analyses as a postdoctoral associate at the USDA/ARS Children's Nutrition Research Center under the support of Grant No. 6250-51000. This research was also supported by funds from the United States Department of Agriculture, Grant No. 2006-55215-16695 and, in part, with funds from Kraft, Inc. This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas and had been funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 6250-51000-053. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. NR 55 TC 13 Z9 13 U1 7 U2 37 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD MAY 1 PY 2014 VL 76 BP 166 EP 174 DI 10.1016/j.appet.2014.01.077 PG 9 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA AG0JZ UT WOS:000335103100022 PM 24533968 ER PT J AU Fagerquist, CK Zaragoza, WJ Sultan, O Woo, N Quinones, B Cooley, MB Mandrell, RE AF Fagerquist, Clifton K. Zaragoza, William J. Sultan, Omar Woo, Nathan Quinones, Beatriz Cooley, Michael B. Mandrell, Robert E. TI Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight Mass Spectrometry SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID IMMUNO-PCR ASSAY; MONOCLONAL-ANTIBODIES; RAPID IDENTIFICATION; PROTEIN BIOMARKERS; VIRULENCE FACTORS; STRAIN B2F1; VARIANTS; FOOD; BACTERIOPHAGE; OUTBREAKS AB We have analyzed 26 Shiga toxin-producing Escherichia coli (STEC) strains for Shiga toxin 2 (Stx2) production using matrix-assisted laser desorption ionization (MALDI)-tandem time of flight (TOF-TOF) tandem mass spectrometry (MS/MS) and top-down proteomic analysis. STEC strains were induced to overexpress Stx2 by overnight culturing on solid agar supplemented with either ciprofloxacin or mitomycin C. Harvested cells were lysed by bead beating, and unfractionated bacterial cell lysates were ionized by MALDI. The A2 fragment of the A subunit and the mature B subunit of Stx2 were analyzed by MS/MS. Sequence-specific fragment ions were used to identify amino acid subtypes of Stx2 using top-down proteomic analysis using software developed in-house at the U. S. Department of Agriculture (USDA). Stx2 subtypes (a,c,d,f, and g) were identified on the basis of the mass of the A2 fragment and the B subunit as well as from their sequence-specific fragment ions by MS/MS (postsource decay). Top-down proteomic identification was in agreement with DNA sequencing of the full Stx2 operon (stx(2)) for all strains. Top-down results were also compared to a bioassay using a Vero-d2EGFP cell line. Our results suggest that top-down proteomic identification is a rapid, highly specific technique for distinguishing Stx2 subtypes. C1 [Fagerquist, Clifton K.; Zaragoza, William J.; Sultan, Omar; Woo, Nathan; Quinones, Beatriz; Cooley, Michael B.; Mandrell, Robert E.] USDA ARS, Produce Safety Microbiol Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. RP Fagerquist, CK (reprint author), USDA ARS, Produce Safety Microbiol Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. EM clifton.fagerquist@ars.usda.gov FU USDA-ARS CRIS project [5325-42000-047-00D] FX This research was supported by USDA-ARS CRIS project 5325-42000-047-00D. NR 51 TC 14 Z9 14 U1 3 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2014 VL 80 IS 9 BP 2928 EP 2940 DI 10.1128/AEM.04058-13 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AF3AI UT WOS:000334583300033 PM 24584253 ER PT J AU Yeh, HY Hiett, KL Line, JE Seal, BS AF Yeh, Hung-Yueh Hiett, Kelli L. Line, John E. Seal, Bruce S. TI Characterization and reactivity of broiler chicken sera to selected recombinant Campylobacter jejuni chemotactic proteins SO ARCHIVES OF MICROBIOLOGY LA English DT Article DE Campylobacter jejuni; Poultry; Chemotaxis; epsilon-Proteobacteria; Foodborne pathogen; Zoonoses; Methyl-accepting chemotaxis proteins ID ANTIGEN TECHNOLOGY IVIAT; NUCLEAR-MAGNETIC-RESONANCE; ENERGY TAXIS SYSTEM; BACTERIAL CHEMOTAXIS; IN-VIVO; ESCHERICHIA-COLI; HUMAN INFECTION; CHEMOSENSORY RECEPTOR; MATERNAL ANTIBODIES; SIGNAL-TRANSDUCTION AB Campylobacter jejuni, a Gram-negative rod bacterium, is the leading causative agent of human acute bacterial gastroenteritis worldwide. Consumption and handling of raw or undercooked poultry are regarded as a major source for human infection. Because bacterial chemotaxis guides microorganisms to colonization and invasion in the host cells, proteins involved in chemotactic processes can be novel targets for vaccine development. In this communication, we report amplification, cloning and expression of the C. jejuni chemotactic proteins in an Escherichia coli expression system. A total of 15 chemotactic protein genes were successfully expressed. These recombinant proteins were confirmed by nucleotide sequencing, SDS-PAGE analysis and immunoblot analysis of six-His and hemagglutinin tags. Twelve recombinant chemotactic proteins were further tested whether they were antigenic using sera from broiler chickens older than 4 weeks. The immunoblot results show that each chicken serum reacted to a variety of the recombinant proteins, but all sera reacted to the Cjj0473 gene product (annotated as a methyl-accepting chemotaxis protein), suggesting that anti-Campylobacter antibodies may be prevalent in the poultry population. These antibody screening results provide a rationale for further evaluation of the Cjj0473 protein as a potential vaccine for broilers to improve human food safety. C1 [Yeh, Hung-Yueh; Hiett, Kelli L.; Line, John E.; Seal, Bruce S.] USDA ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. RP Yeh, HY (reprint author), USDA ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS Project [6612-32000-060-00]; U.S. Poultry and Egg Association Project [679] FX We thank Susan Q. Brooks of Poultry Microbiological Safety Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Athens, GA for the excellent technical support. We also thank Dr. Brian E. Scheffler and his Bioinformatics Group at the USDA ARS Genomics and Bioinformatics Research Unit in Stoneville, MS for sequencing and bioinformatics. This study was supported by the USDA Agricultural Research Service CRIS Project No. 6612-32000-060-00 and the U.S. Poultry and Egg Association Project No. 679. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 64 TC 3 Z9 3 U1 1 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0302-8933 EI 1432-072X J9 ARCH MICROBIOL JI Arch. Microbiol. PD MAY PY 2014 VL 196 IS 5 BP 375 EP 383 DI 10.1007/s00203-014-0969-z PG 9 WC Microbiology SC Microbiology GA AF0OB UT WOS:000334412800007 PM 24609189 ER PT J AU Atehnkeng, J Ojiambo, PS Cotty, PJ Bandyopadhyay, R AF Atehnkeng, J. Ojiambo, P. S. Cotty, P. J. Bandyopadhyay, R. TI Field efficacy of a mixture of atoxigenic Aspergillus flavus Link: Fr vegetative compatibility groups in preventing aflatoxin contamination in maize (Zea mays L.) SO BIOLOGICAL CONTROL LA English DT Article DE Native strains; Aspergillus section Flavi; Aspergillus parasiticus; Aspergillus tamarii; Nigeria ID 3 AGROECOLOGICAL ZONES; BIOLOGICAL-CONTROL; BIOCONTROL AGENTS; GENETIC DIVERSITY; SECTION FLAVI; POPULATION; KERNELS; NIGERIA; PARASITICUS; MYCOTOXINS AB Competitive exclusion of aflatoxin producers by endemic atoxigenic strains of Aspergillus flavus is a proven tool for aflatoxin management being adapted for use in Africa. Field efficacy of an experimental formulation consisting of four native atoxigenic strains (La3303, La3304, La3279 and Ka16127) was evaluated on maize in 2007 and 2008 in four agroecological zones in Nigeria. The four atoxigenic strains were individually formulated on sterile sorghum grain and subsequently mixed in equal proportions. The blended product was applied on soil (40 kg/ha), 2-3 weeks before flowering. Grains from treated and untreated fields were analyzed for aflatoxins at harvest and after storage. Proportions of the A. flavus population composed of each of the four applied strains in soil before treatment and in harvested grains were determined using vegetative compatibility analyses. Application of the strain mixture resulted in reduced aflatoxin content and significantly (P < 0.05) increased the combined frequencies of the vegetative compatibility groups (VCGs) of the applied strains recovered from the soil and grain. Aflatoxin reductions of 67-95% were associated with a 74-80% combined incidence of the VCGs of the four atoxigenic strains on the treated crops. The applied atoxigenic strains remained with the crop into storage and reduced post-harvest increases in contamination. The results suggest that the evaluated multi-strain formulated product has potential to contribute to reduced aflatoxin contamination in Nigeria. This is the first report of a field evaluation of an endemic strain mixture effective at reducing aflatoxin contamination during crop development. (C) 2014 Elsevier Inc. All rights reserved. C1 [Atehnkeng, J.; Bandyopadhyay, R.] Int Inst Trop Agr, Ibadan, Nigeria. [Ojiambo, P. S.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Cotty, P. J.] Univ Arizona, USDA ARS, Sch Plant Sci, Tucson, AZ 85721 USA. RP Bandyopadhyay, R (reprint author), IITA, Carolyn House,26 Dingwall Rd, Croydon CR9 3EE, England. EM r.bandyopadhyay@cgiar.org FU German Federal Ministry for Economic Cooperation and Development [2002.7860.6-001.00] FX This study was funded by the German Federal Ministry for Economic Cooperation and Development Project No. 2002.7860.6-001.00. NR 45 TC 11 Z9 11 U1 0 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2014 VL 72 BP 62 EP 70 DI 10.1016/j.biocontrol.2014.02.009 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF6UH UT WOS:000334849600008 ER PT J AU Milbrath, LR Nechols, JR AF Milbrath, Lindsey R. Nechols, James R. TI Plant-mediated interactions: Considerations for agent selection in weed biological control programs SO BIOLOGICAL CONTROL LA English DT Article DE Indirect interactions; Weed biological control; Pre-release evaluation; Agent selection ID RHINOCYLLUS-CONICUS COLEOPTERA; TRICHOSIROCALUS-HORRIDUS; CARDUUS-NUTANS; INTERSPECIFIC INTERACTIONS; CENTAUREA-SOLSTITIALIS; ALLIARIA-PETIOLATA; PLUMELESS THISTLES; BIOCONTROL AGENTS; LYTHRUM-SALICARIA; DIFFUSE KNAPWEED AB Plant-mediated indirect interactions among herbivores (arthropods and pathogens) are common and extensively reported in the ecological literature. However, they are not well-documented with respect to weed biological control. Such interactions between biological control agents can have net positive or negative impacts on total weed suppression depending on the strength of the interaction(s), the relative importance of the agent indirectly impacted, and the combined weed suppression that results. A better understanding of plant-mediated interactions may improve decision-making about which agents to introduce in classical biological control programs for greatest impact on invasive weeds. This paper reviews the subject, including examples from the biological control literature; outlines the need for research on indirect effects of herbivores on other herbivores; discusses how such knowledge may strengthen classical biological control programs for invasive weeds; and provides recommendations for the kind of studies that should be done and how information about plant-mediated interactions could be integrated into agent evaluation protocols, to assist in decision-making about agents for importation and release. Published by Elsevier Inc. C1 [Milbrath, Lindsey R.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Nechols, James R.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Milbrath, LR (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM Lindsey.Milbrath@ars.usda.gov NR 81 TC 3 Z9 3 U1 3 U2 40 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2014 VL 72 BP 80 EP 90 DI 10.1016/j.biocontrol.2014.02.011 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF6UH UT WOS:000334849600011 ER PT J AU Goble, TA Rehner, SA Long, SJ Gardescu, S Hajek, AE AF Goble, Tarryn A. Rehner, Stephen A. Long, Stefan J. Gardescu, Sana Hajek, Ann E. TI Comparing virulence of North American Beauveria brongniartii and commercial pathogenic fungi against Asian longhorned beetles SO BIOLOGICAL CONTROL LA English DT Article DE Microbial control; Entomopathogenic fungus; Beauveria asiatica; Metarhizium brunneum; Bioassay; Inoculation technique ID ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; IN-VITRO PASSAGE; ENTOMOPATHOGENIC FUNGUS; FIBER BANDS; CERAMBYCIDAE; BASSIANA; DEUTEROMYCOTINA; ACQUISITION; EXPOSURE AB In the USA, the development and field application of Beauveria brongniartii (Sacc.) Petch (Hypocreales: Clavicipitaceae) to control the invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae) have been hampered because it was unknown whether this fungal species is native to North America. With the recent confirmation of the occurrence of B. brongniartii in North America there is renewed interest in this species, particularly as it is an effective pathogen of cerambycids in Japan. However, based on partial sequences of the nuclear intergenic BLOC region the commercially available B. brongniartii strain NBL 851 (Idemitsu Kosan, Tokyo, Japan) belongs instead to the species Beauveria asiatica Rehner and Humber. Further, bioassays using two inoculation methods confirmed that commercially available strains of B. asiatica (NBL 851) and Metarhizium brunneum (F52) (Hypocreales: Clavicipitaceae) were significantly more virulent and resulted in lower median survival times (9.5-7.5 d) of A. glabripennis adults than two North American B. brongniartii isolates (ARSEF 6215 and ARSEF 10279) (24-31 d). The virulence of North American B. brongniartii isolates is not well-documented in the literature. To our best knowledge this is the first account of the virulence of native North American B. brongniartii being evaluated for biological control of any invasive insect pest. (C) 2014 Elsevier Inc. All rights reserved. C1 [Goble, Tarryn A.; Long, Stefan J.; Gardescu, Sana; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Rehner, Stephen A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Goble, TA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM tazgoble@gmail.com FU Alphawood Foundation; Litwin Foundation FX We thank our laboratory assistants and technicians, especially Meghan Roblee, Jake Henry and George Lu. The Alphawood Foundation supported the beetle colony and the Litwin Foundation provided research support. NR 29 TC 4 Z9 4 U1 2 U2 32 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2014 VL 72 BP 91 EP 97 DI 10.1016/j.biocontrol.2014.02.006 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF6UH UT WOS:000334849600012 ER PT J AU Zhang, QH Zhang, J Yang, L Zhang, L Jiang, DH Chen, WD Li, GQ AF Zhang, Qinghua Zhang, Jing Yang, Long Zhang, Lei Jiang, Daohong Chen, Weidong Li, Guoqing TI Diversity and biocontrol potential of endophytic fungi in Brassica napus SO BIOLOGICAL CONTROL LA English DT Article DE Brassica napus; Endophytic fungi; Volatile organic compounds; Sclerotinia sclerotiorum; Botrytis cinerea; Biological control ID SCLEROTINIA-SCLEROTIORUM; PIRIFORMOSPORA-INDICA; CHAETOMIUM-GLOBOSUM; GLIOCLADIUM-ROSEUM; FUSARIUM-OXYSPORUM; BIOLOGICAL-CONTROL; DISEASE; SUPPRESSION; AGENT; CROPS AB This study was conducted to isolate endophytic fungi from oilseed rape (Brassica napus), to identify the fungal endophytes based on morphology and ITS (ITS1-5.8S rDNA-ITS2) sequences, and to evaluate their efficacy in suppression of the plant pathogenic fungi Sclerotinia sclerotiorum and Botrytis cinerea. Selected endophytic fungal isolates were further tested for promoting growth of oilseed rape in potting experiments. A total of 97 endophytic fungal isolates were obtained from roots (35), stems (49) and leaves (13) of B. napus. Forty fungal species were identified and most species (80%) belong to Ascomycota. The species composition is highly diversified with Simpson's diversity index reaching 0.959. Alternaria alternata is the dominant species accounting for 12.4% of the isolates. Twenty-four isolates exhibited antifungal activity against S. sclerotiorum in dual cultures on potato dextrose agar forming inhibition zones of 3-17 mm in width. The culture filtrates of Aspergillus flavipes CanS-34A, Chaetomium globosum CanS-73, Clonostachys rosea CanS-43 and Leptosphaeria biglobosa CanS-51 in potato dextrose broth exhibited consistent and effective suppression of oilseed rape leaf blight caused by S. sclerotiorum. Fusarium oxysporum CanR-46 was detected capable of production of volatile organic compounds highly inhibitory to S. sclerotiorum and B. cinerea. Moreover, A. alternata CanL-18, Fusarium tricinctum CanR-70 and CanR-71r, and L. biglobosa CanS-51 exhibited growth-promoting effects on oilseed rape. These results suggest that B. napus harbors diversified endophytic fungi, from which potential biocontrol agents against S. sclerotiorum and B. cinerea, and for promoting growth of B. napus can be screened. (C) 2014 Elsevier Inc. All rights reserved. C1 [Zhang, Qinghua; Zhang, Jing; Yang, Long; Jiang, Daohong; Li, Guoqing] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. [Zhang, Qinghua; Zhang, Jing; Yang, Long; Jiang, Daohong; Li, Guoqing] Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Peoples R China. [Zhang, Lei] Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China. [Chen, Weidong] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Li, GQ (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China. EM guoqingli@mail.hzau.edu.cn FU R&D Special Fund for Public Welfare Industry (Agriculture) of China, China [201103016, 201303025]; National Natural Science Foundation of China, China [31070122] FX This research was funded by the R&D Special Fund for Public Welfare Industry (Agriculture) of China, China (Grant Nos. 201103016, 201303025) and the National Natural Science Foundation of China, China (Grant No. 31070122). NR 48 TC 14 Z9 15 U1 4 U2 125 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2014 VL 72 BP 98 EP 108 DI 10.1016/j.biocontrol.2014.02.018 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AF6UH UT WOS:000334849600013 ER PT J AU Zhou, ZG Cao, XY Schmidt-Rohr, K Olk, DC Zhuang, SY Zhou, J Cao, ZH Mao, JD AF Zhou, Zhigao Cao, Xiaoyan Schmidt-Rohr, Klaus Olk, Daniel C. Zhuang, Shunyao Zhou, Jing Cao, Zhihong Mao, Jingdong TI Similarities in chemical composition of soil organic matter across a millennia-old paddy soil chronosequence as revealed by advanced solid-state NMR spectroscopy SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Soil organic matter; Chemical composition; Paddy soil chronosequence; NMR; FTIR ID HUMIC SUBSTANCES; CARBON; CHINA; CULTIVATION; C-13; MINERALIZATION; STABILIZATION; RECALCITRANCE; ACCUMULATION; MANAGEMENT AB This study examined the chemical composition of soil organic matter (SOM) along a 2,000-year paddy soil chronosequence in eastern China by use of advanced solid-state nuclear magnetic resonance (NMR) spectroscopy as well as Fourier transform infrared spectroscopy (FTIR), aiming to identify changes in the chemical composition of SOM over a millennium timescale. The results showed that soil organic carbon concentration in the surface soil reached a steady state after 100 years of rice (Oryza sativa L.)-wheat (Triticum sp.) cropping on coastal tidal flats. The C-13 NMR spectra and fractions of structural groups or components of the whole SOM samples differed little along the chronosequence, suggesting a similar chemical composition in SOM samples regardless of the duration of rice cultivation. The FTIR spectral pattern and relative intensities of some resolved functional groups or components of whole SOM were also similar along the soil chronosequence. The similarities in chemical composition of SOM can be attributed to the rice-wheat cropping system, in which SOM has undergone ongoing turnover under periodical fresh plant material input and wet-dry cropping alternation, leading to a similar chemical composition of bulk SOM. C1 [Zhou, Zhigao; Cao, Xiaoyan; Mao, Jingdong] Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. [Schmidt-Rohr, Klaus] Iowa State Univ, Dept Chem, Ames, IA 50011 USA. [Olk, Daniel C.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Zhou, Zhigao; Zhuang, Shunyao; Zhou, Jing; Cao, Zhihong] Chinese Acad Sci, Inst Soil Sci, Nanjing 210008, Jiangsu, Peoples R China. [Zhou, Zhigao; Cao, Xiaoyan; Mao, Jingdong] Nanjing Agr Univ, Dept Chem, Coll Sci, Nanjing 210095, Jiangsu, Peoples R China. RP Mao, JD (reprint author), Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. EM maojd@njau.edu.cn RI Cao, Xiaoyan/E-3492-2012 OI Cao, Xiaoyan/0000-0001-7571-6482 FU National Science Foundation [EAR-1226323] FX We would like to thank the National Science Foundation (EAR-1226323) for partial support. NR 53 TC 2 Z9 2 U1 2 U2 40 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 EI 1432-0789 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD MAY PY 2014 VL 50 IS 4 BP 571 EP 581 DI 10.1007/s00374-013-0875-6 PG 11 WC Soil Science SC Agriculture GA AF7ZR UT WOS:000334935000002 ER PT J AU Lucash, MS Scheller, RM Kretchun, AM Clark, KL Hom, J AF Lucash, Melissa S. Scheller, Robert M. Kretchun, Alec M. Clark, Kenneth L. Hom, John TI Impacts of fire and climate change on long-term nitrogen availability and forest productivity in the New Jersey Pine Barrens SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Century; forest simulation model; LANDIS-II; nitrogen cycle ID UNITED-STATES; MINERALIZATION RATES; CHANGE SCENARIOS; CARBON DYNAMICS; PONDEROSA PINE; FREQUENT FIRE; SHRUBLANDS; ECOSYSTEM; CO2; TRANSFORMATIONS AB Increased wildfires and temperatures due to climate change are expected to have profound effects on forest productivity and nitrogen (N) cycling. Forecasts about how wildfire and climate change will affect forests seldom consider N availability, which may limit forest response to climate change, particularly in fire-prone landscapes. The overall objective of this study was to examine how wildfire and climate change affect long-term mineral N availability in a fire-prone landscape. We employed a commonly used landscape simulation model (LANDIS-II) in the New Jersey Pine Barrens, a landscape characterized by frequent small fires and fire-resilient vegetation. We found that fire had little effect on mineral N, whereas climate change and fire together reduced mineral N by the end of the century. Though N initially limited forest productivity, mineral N was no longer limiting after 50 years. Our results suggest that mineral N is resilient to fire under our current climate but not under climate change. Also, predictions that do not consider N limitation may underestimate short-term but not long-term productivity responses to climate change. Together these results illustrate the importance of including N dynamics when simulating the effects of climate change on forest productivity, particularly in fire-prone regions such as the New Jersey Pine Barrens. C1 [Lucash, Melissa S.; Scheller, Robert M.; Kretchun, Alec M.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97201 USA. [Clark, Kenneth L.] US Forest Serv, USDA, Silas Little Expt Forest, New Lisbon, NJ 08064 USA. [Hom, John] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. RP Lucash, MS (reprint author), Portland State Univ, Dept Environm Sci & Management, Portland, OR 97201 USA. EM lucash@pdx.edu RI Scheller, Robert/B-3135-2009 FU Forest Health Monitoring Plan [NE-F-0-01, NE-F-08-01] FX Funding was provided by the Forest Health Monitoring Plan (NE-F-0-01 and NE-F-08-01). NR 60 TC 0 Z9 0 U1 4 U2 29 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2014 VL 44 IS 5 BP 404 EP 412 DI 10.1139/cjfr-2013-0383 PG 9 WC Forestry SC Forestry GA AF6UE UT WOS:000334849300002 ER PT J AU Samuelson, LJ Stokes, TA Butnor, JR Johnsen, KH Gonzalez-Benecke, CA Anderson, P Jackson, J Ferrari, L Martin, TA Cropper, WP AF Samuelson, Lisa J. Stokes, Tom A. Butnor, John R. Johnsen, Kurt H. Gonzalez-Benecke, Carlos A. Anderson, Pete Jackson, Jason Ferrari, Lorenzo Martin, Tim A. Cropper, Wendell P., Jr. TI Ecosystem carbon stocks in Pinus palustris forests SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE longleaf pine; carbon sequestration; allometry; roots; ground-penetrating radar ID LONGLEAF PINE; UNITED-STATES; LOBLOLLY-PINE; BIOMASS EQUATIONS; ROOT; CHRONOSEQUENCE; MANAGEMENT; PATTERNS; STORAGE; ALLOCATION AB Longleaf pine (Pinus palustris Mill.) restoration in the southeastern United States offers opportunities for carbon (C) sequestration. Ecosystem C stocks are not well understood in longleaf pine forests, which are typically of low density and maintained by prescribed fire. The objectives of this research were to develop allometric equations for above-and below-ground biomass and quantify ecosystem C stocks in five longleaf pine forests ranging in age from 5 to 87 years and in basal area from 0.4 to 22.6 m(2).ha(-1). Live aboveground C (woody plant + ground cover) and live root C (longleaf pine below stump + plot level coarse roots + plot level fine roots) ranged from 1.4 and 2.9 Mg C.ha(-1), respectively, in the 5-year-old stand to 78.4 and 19.2 Mg C.ha(-1), respectively, in the 87-year-old stand. Total ecosystem C (live plant + dead organic matter + mineral soil) values were 71.6, 110.1, 124.6, 141.4, and 185.4 Mg C.ha(-1) in the 5-, 12-, 21-, 64-, and 87-year-old stands, respectively, and dominated by tree C and soil C. In the 5-year-old stand, ground cover C and residual taproot C were significant C stocks. This unique, in-depth assessment of above- and below-ground C across a series of longleaf pine stands will improve estimates of C in longleaf pine ecosystems and contribute to development of general biomass models that account for variation in climate, site, and management history in an important but understudied ecosystem. C1 [Samuelson, Lisa J.; Stokes, Tom A.; Ferrari, Lorenzo] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Butnor, John R.; Johnsen, Kurt H.; Anderson, Pete; Jackson, Jason] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 28809 USA. [Gonzalez-Benecke, Carlos A.; Martin, Tim A.; Cropper, Wendell P., Jr.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. RP Samuelson, LJ (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 3301 SFWS Bldg, Auburn, AL 36849 USA. EM samuelj@auburn.edu RI Cropper, Jr., Wendell/E-5952-2010; Butnor, John/P-9738-2016; OI Cropper, Jr., Wendell/0000-0001-7851-7382; Martin, Timothy/0000-0002-7872-4194 FU U.S. Department of Defense FX This research was supported wholly (or in part) by the U.S. Department of Defense through the Strategic Environmental Research and Development Program (SERDP). The authors thank Jake Blackstock, Justin Rathel, Patrick Steele, Liz Jordon, Tyler Bowden, Brad Stone, Michael Gunter, and Adam Kelley for assistance in data collection and James Parker and Brian Waldrep from Fort Benning, Land Management Division, for their assistance. We appreciate the technical support provided by Joel Burley, Thomas Christensen, Robert Eaton, Shelly Hooke, and Karen Sarsony. NR 53 TC 10 Z9 10 U1 6 U2 56 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2014 VL 44 IS 5 BP 476 EP 486 DI 10.1139/cjfr-2013-0446 PG 11 WC Forestry SC Forestry GA AF6UE UT WOS:000334849300009 ER PT J AU Sigua, GC Novak, JM Watts, DW Cantrell, KB Shumaker, PD Szogi, AA Johnson, MG AF Sigua, G. C. Novak, J. M. Watts, D. W. Cantrell, K. B. Shumaker, P. D. Szoegi, A. A. Johnson, M. G. TI Carbon mineralization in two ultisols amended with different sources and particle sizes of pyrolyzed biochar SO CHEMOSPHERE LA English DT Article DE Biochar; Mineralization; Pellets; Ultisols; Norfolk soil; Coxville soil ID BLACK CARBON; SOIL; CHARCOAL; TEMPERATURE; OXIDATION; SEQUESTRATION; DECOMPOSITION; BIOENERGY; AMENDMENT; IMPACT AB Biochar produced during pyrolysis has the potential to enhance soil fertility and reduce greenhouse gas emissions. The influence of biochar properties (e.g., particle size) on both short- and long-term carbon (C) mineralization of biochar remains unclear. There is minimal information on the potential effects of biochar particle sizes on their breakdowns by soil microorganism, so it is unknown if the particle size of biochar influences C mineralization rate and/or stability in soils. In order to evaluate the effect of different sources (BS) and particle sizes (BF) of biochar on C loss and/or stability in soils, an incubation study on C mineralization of different biochar sources and particle sizes was established using two soils (ST): Norfolk soil (fine loamy, kaolinitic, thermic, typic Kandiudults) and Coxville soil (fine loamy kaolinitic, thermic, Paleaquults). In separate incubation vessels, these soils were amended with one of two manure-based biochars (poultry litters, PL; swine solids, SS) or one of two lignocellulosic-based biochars (switchgrass, SG; pine chips, PC) which were processed into two particle sizes (dust, <0.42 mm; pellet, >2 mm). The amount of CO2 evolved varied significantly between soils (p <= 0.0001); particle sizes (p <= 0.0001) and the interactions of biochar source (p <= 0.001) and forms of biochars (p <= 0.0001) with soil types. Averaged across soils and sources of biochar, CO2-C evolved from dust-sized biochar (281 mg kg(-1)) was significantly higher than pellet-sized biochar (226 mg kg(-1)). Coxville soils with SS biochar produced the greatest average CO2-C of 428 mg kg(-1) and Norfolk soils with PC had the lowest CO2-C production (93 mg kg(-1)). Measured rates of carbon mineralization also varied with soils and sources of biochar (Norfolk: PL > SS > SG PC; Coxville: PC > SG > SS > PL). The average net CO2-C evolved from the Coxville soils (385 mg kg(-1)) was about threefold more than the CO2-C evolved from the Norfolk soils (123 mg kg(-1)). Our results suggest different particle sizes and sources of biochar as well as soil type influence biochar stability. Published by Elsevier Ltd. C1 [Sigua, G. C.; Novak, J. M.; Watts, D. W.; Cantrell, K. B.; Shumaker, P. D.; Szoegi, A. A.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Johnson, M. G.] US EPA, Natl Hlth & Environm Effects Res Lab, Western Ecol Div, Corvallis, OR 97333 USA. RP Sigua, GC (reprint author), USDA ARS CPSWPRC, 2611 West Lucas St, Florence, SC 29501 USA. EM gilbert.sigua@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service [60-6657-1-204]; United States Environmental Protection Agency [DE-12-92342301-1] FX The information in this article has been funded through an Interagency Agreement between the United States Department of Agriculture-Agricultural Research Service (60-6657-1-204) and the United States Environmental Protection Agency (DE-12-92342301-1). It has been subject to review by scientists of the USDA-ARS-Coastal Plain Research Laboratory and by the National Health and Environment Effects Research Laboratory's Western Ecology Division and approved for journal submission. Approval does not signify that the contents reflect views of the US EPA, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We thank Carl Trettin for assistance with feedstock collection and Cierra Buckman, Takeyah Powell, and Mr. Jerry Martin II for laboratory assistance. NR 44 TC 28 Z9 28 U1 2 U2 95 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAY PY 2014 VL 103 BP 313 EP 321 DI 10.1016/j.chemosphere.2013.12.024 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AF1NZ UT WOS:000334481900040 PM 24397887 ER PT J AU Mueller, GA Maleki, SJ Pedersen, LC AF Mueller, Geoffrey A. Maleki, Soheila J. Pedersen, Lars C. TI The Molecular Basis of Peanut Allergy SO CURRENT ALLERGY AND ASTHMA REPORTS LA English DT Review DE Peanut allergy; Molecular basis; Peanut allergens; Major allergens; Minor allergens; Protein structures; Cross-reactivity; Molecular modifications ID GLYCATION END-PRODUCTS; ARA H 2; PLANT FOOD ALLERGENS; IGE-BINDING EPITOPES; CRYSTAL-STRUCTURE; ARACHIS-HYPOGAEA; IMMUNOGLOBULIN-E; CARBOHYDRATE DETERMINANTS; FUNCTIONAL-ASPECTS; TRYPSIN-INHIBITOR AB Peanut allergens can trigger a potent and sometimes dangerous immune response in an increasing number of people. The molecular structures of these allergens form the basis for understanding this response. This review describes the currently known peanut allergen structures and discusses how modifications both enzymatic and non-enzymatic affect digestion, innate immune recognition, and IgE interactions. The allergen structures help explain cross-reactivity among allergens from different sources, which is useful in improving patient diagnostics. Surprisingly, it was recently noted that similar short peptide sequences among unrelated peanut allergens could also be a source of cross-reactivity. The molecular features of peanut allergens continue to inform predictions and provide new research directions in the study of allergic disease. C1 [Mueller, Geoffrey A.; Pedersen, Lars C.] NIEHS, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. [Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. RP Mueller, GA (reprint author), NIEHS, Struct Biol Lab, 111 TW Alexander Dr,MD-MR-01, Res Triangle Pk, NC 27709 USA. EM Mueller3@niehs.nih.gov FU National Institute of Environmental Health Sciences, National Institutes of Health [Z01-ES102885-01, ZIA-ES102645] FX The authors wish to thank Drs. Robert London, Michael Fessler, and Jason Williams for critical readings of the manuscript. This research was supported by Research Project Number Z01-ES102885-01 and ZIA-ES102645 in the Intramural Research Program of the National Institute of Environmental Health Sciences, National Institutes of Health. NR 81 TC 4 Z9 5 U1 5 U2 37 PU CURRENT MEDICINE GROUP PI PHILADELPHIA PA 400 MARKET STREET, STE 700, PHILADELPHIA, PA 19106 USA SN 1529-7322 EI 1534-6315 J9 CURR ALLERGY ASTHM R JI Curr. Allergy Asthma Rep. PD MAY PY 2014 VL 14 IS 5 AR 429 DI 10.1007/s11882-014-0429-5 PG 9 WC Allergy; Immunology SC Allergy; Immunology GA AE7KS UT WOS:000334177300001 PM 24633613 ER PT J AU Nouwakpo, SK Huang, CH Weltz, MA Pimenta, F Chagas, I Lima, L AF Nouwakpo, Sayjro Kossi Huang, Chi-hua Weltz, Mark A. Pimenta, Flavia Chagas, Isis Lima, Luiz TI Using fluidized bed and flume experiments to quantify cohesion development from aging and drainage SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE drainage; soil cohesion; soil subsurface hydrology; seepage; soil consolidation; critical shear stress; soil erodibility; fluidized bed; soil aging ID CRITICAL SHEAR-STRESS; AGGREGATE STABILITY; WATER-CONTENT; HYDRAULIC-GRADIENT; SOIL COHESION; RILL EROSION; TEMPERATURE; ERODIBILITY; CONSOLIDATION; RESISTANCE AB Temporal variations in soil erosion resistance are often the result of decreased soil cohesion due to physical disruption followed by a regain of soil strength through a process called aging, stabilization or consolidation. The goal of this study was to quantify changes in soil cohesion due to aging and subsurface hydrologic condition using a fluidized bed method. A flume experiment was also used to verify that findings from the fluidized bed experiment translated into measurable changes in soil erodibility. Tests were performed on three different soils (a Miami soil, a Cecil soil and Crosby-Miami soil complex). Changes in soil cohesion due to aging and drainage state were successfully detected by the fluidized bed technique. For all soils tested, cohesion developed in a two-stage process where an increase in cohesion with aging duration immediately after the soil was rewetted, was followed by a decrease in cohesion which often started after 24 h of aging. When soils were aged at field capacity, the resulting cohesion measured by the fluidized bed method was on average 3.13 times higher than that measured when aging was performed at saturation. Trends in soil rill erodibility K-r with aging duration measured in the flume experiment were consistent with the two-stage pattern observed in soil cohesion estimates but the legacy effect of suction applied at field capacity faded after 72 h of aging. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Nouwakpo, Sayjro Kossi] Univ Nevada, Reno, NV 89557 USA. [Huang, Chi-hua] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Weltz, Mark A.] ARS, USDA, Exot & Invas Weeds Res Unit, Reno, NV USA. [Pimenta, Flavia; Chagas, Isis; Lima, Luiz] Univ Fed Lavras, BR-3037 Lavras, Brazil. RP Nouwakpo, SK (reprint author), Univ Nevada, 1664 North Virginia St, Reno, NV 89557 USA. EM snouwakpo@unr.edu NR 27 TC 0 Z9 0 U1 2 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD MAY PY 2014 VL 39 IS 6 BP 749 EP 757 DI 10.1002/esp.3477 PG 9 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA AF3XO UT WOS:000334646200004 ER PT J AU Baldwin, RA Quinn, N Davis, DH Engeman, RM AF Baldwin, Roger A. Quinn, Niamh Davis, David H. Engeman, Richard M. TI Effectiveness of rodenticides for managing invasive roof rats and native deer mice in orchards SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Bait station; Chlorophacinone; Deer mouse; Diphacinone; Human-wildlife conflict; Peromyscus; maniculatus; Rattus rattus; Roof rat ID SQUIRRELS SPERMOPHILUS-BEECHEYI; POPULATIONS; CALIFORNIA; ABUNDANCE; INDEXES; CONSERVATION; ERADICATION; DIPHACINONE; MANAGEMENT; PARADIGM AB Roof rats (Rattus rattus) and deer mice (Peromyscus maniculatus) are occasional pests of nut and tree fruit orchards throughout California and in many other parts of the USA and beyond. In general, the most practical and cost-effective control method for rodents in many agricultural environments is the use of rodenticides (toxic baits), but little or no information exists on the efficacy of current rodenticides in controlling roof rats and deer mice in orchards. Therefore, our goals were to develop an index of rodent activity to monitor efficacy of rodenticides and to subsequently test the efficacy of three California Department of Food and Agriculture rodenticide baits (0.005 % chlorophacinone treated oats, 0.005 % diphacinone treated oats, and 0.005 % diphacinone wax block) to determine their utility for controlling roof rats and deer mice in agricultural orchards. We determined that a general index using the number of roof rat photos taken at a minimum of a 5-min interval was strongly correlated to the minimum number known estimate of roof rats; this approach was used to monitor roof rat and deer mouse populations pre- and post-treatment. Of the baits tested, the 0.005 % diphacinone treated oats was most effective for both species; 0.005 % chlorophacinone grain was completely ineffective against roof rats. Our use of elevated bait stations proved effective at providing bait to target species and should substantially limit access to rodenticides by many non-target species. C1 [Baldwin, Roger A.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95695 USA. [Quinn, Niamh; Davis, David H.] Univ Calif, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. [Engeman, Richard M.] Natl Wildlife Res Ctr, USDA Wildlife Serv, Ft Collins, CO 80521 USA. RP Baldwin, RA (reprint author), Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, 1 Shields Ave, Davis, CA 95695 USA. EM rabaldwin@ucdavis.edu FU Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture FX We would like to thank numerous landowners for providing access to their property for this study and to H. Jantz and D. Stetson for valuable field assistance. We also thank G. Creekmur and S. Neblett for assistance in selecting an appropriate bait station design and F. Rinder and R. Lantsberger for thoughtful discussions on this project. This project was funded by the Vertebrate Pest Control Research Advisory Committee of the California Department of Food and Agriculture. NR 28 TC 2 Z9 2 U1 3 U2 30 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD MAY PY 2014 VL 21 IS 9 BP 5795 EP 5802 DI 10.1007/s11356-014-2525-4 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AF4LS UT WOS:000334684700004 PM 24443051 ER PT J AU Johnson, TN Buskirk, SW Hayward, GD Raphael, MG AF Johnson, Tracey N. Buskirk, Steven W. Hayward, Gregory D. Raphael, Martin G. TI Tree mortality after synchronized forest insect outbreaks: Effects of tree species, bole diameter, and cutting history SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Dendroctonus; Engelmann spruce; Lodgepole pine; Mountain pine beetle; Patch-cutting; Subalpine fir ID MOUNTAIN PINE-BEETLE; SOUTHERN ROCKY-MOUNTAINS; BALSAM BARK BEETLE; STAND CHARACTERISTICS; ALPINE FIR; WESTERN; ATTACK; INFESTATIONS; SCOLYTIDAE; COLEOPTERA AB A recent series of bark beetle outbreaks in the Rocky Mountain region of the U.S. is the largest and most intense ever recorded. Factors contributing to tree mortality from bark beetles are complex, but include aspects of forest stand condition. Because stand conditions respond to forest management, evaluating bark beetle-caused tree mortality and changes in forest structural attributes in areas previously subjected to management not only improves mechanistic understanding of beetle-caused changes in forests, but also improves prediction of future bark beetle responses to management regimes. We retrospectively assessed mortality of lodgepole pine (Pinus contorta), Engelmann spruce (Picea engelmannii), and subalpine fir (Abies lasiocarpa), and stand structure in two watersheds in south-central Wyoming, U.S.A. following outbreaks of mountain pine beetle (Dendroctonus ponderosae), spruce beetle (Dendroctonus rufipennis), and western balsam beetle (Dyocoetes confusus). One watershed received 240 patch cuts (mean area 1.5 ha), a type of group selection cut, six years before the beetle outbreaks began; the other watershed received no active management (control). We conducted surveys of forest vegetation attributes over 27 yrs, during pre-harvest, post-harvest, and post-outbreak periods. After the outbreak, lodgepole pine and Engelmann spruce mortality increased with increasing bole diameters and basal area of each species, but patterns of mortality were influenced by patch-cutting. Large-diameter trees in or near patch cuts tended to escape attack by bark beetles. Away from patch cuts (>= 15 m), mortality of smaller lodgepole pine was higher compared to the control watershed. Based on our observed patterhs of tree mortality, we hypothesize a changing pattern of host selection (i.e., selection for smaller trees) was influenced by stand conditions that created more suitable conditions for bark beetles in areas between patch cuts in the treated watershed. Snag density increased from pre-harvest to post-outbreak periods, but log density was similar, suggesting most dead trees remained standing at the time of data collection. Canopy cover did not decrease as expected, and ground cover did not change substantially from pre-harvest to post-outbreak periods. Patch-cutting improved survival probability of large-diameter lodgepole pine and Engelmann spruce during outbreaks of multiple species of bark beetle, although reduced losses were only realized for trees in or near (<= 15 m) patch cuts. However, during intense, broad-scale tree mortality events, these benefits may be important in reducing the loss of mature trees to bark beetles and promoting retention of a larger cohort of mature trees post-outbreak. (C) 2014 Elsevier B.V. All rights reserved. C1 [Johnson, Tracey N.; Buskirk, Steven W.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Hayward, Gregory D.] US Forest Serv, USDA, Anchorage, AK 99501 USA. [Raphael, Martin G.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Johnson, TN (reprint author), Univ Wyoming, Dept Zool & Physiol, Dept 3166,1000 East Univ Ave, Laramie, WY 82071 USA. EM tjohns67@uwyo.edu RI Buskirk, Steven/K-2871-2016 OI Buskirk, Steven/0000-0003-0611-6648 FU U.S.D.A. Forest Service Cooperative [09-CS-11020600-043]; Rocky Mountain Research Station; U.S.D.A. Forest Service Region 2; Wyoming Game and Fish Department FX Support for this work was provided by U.S.D.A. Forest Service Cooperative Agreement Number 09-CS-11020600-043, Rocky Mountain Research Station, U.S.D.A. Forest Service Region 2, and Wyoming Game and Fish Department. S.E. Henry, G. Brown, D. Winslow, and numerous technicians provided valuable field assistance R.J Cain made helpful contributions to earlier versions of this manuscript. NR 48 TC 5 Z9 5 U1 2 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2014 VL 319 BP 10 EP 17 DI 10.1016/j.foreco.2014.01.047 PG 8 WC Forestry SC Forestry GA AG0HQ UT WOS:000335096900002 ER PT J AU Keyser, TL Zarnoch, SJ AF Keyser, Tara L. Zarnoch, Stanley J. TI Stump sprout dynamics in response to reductions in stand density for nine upland hardwood species in the southern Appalachian Mountains SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Bent Creek Experimental Forest; Central Hardwood Region; Forest thinning; Quercus; Regeneration ID STRUCTURAL DEVELOPMENT; BETULA-PUBESCENS; CUTTING SEASON; FELLING TIME; RED MAPLE; OAK; REGENERATION; FORESTS; HEIGHT; DIAMETER AB Much about stump sprout dynamics of upland hardwood trees species has been obtained in clearcuts. Information on the response of stump sprouts to alternative silvicultural treatments, including treatments that manipulate stand density and stand structure is lacking. In this study we examined the influence of harvest season and levels of basal area reduction on the probability of sprouting and subsequent sprout growth in the southern Appalachian Mountains. In 2009, 24 - 0.1 ha plots were established in fully-stocked mixed-hardwood forests near Asheville, North Carolina, USA. Basal area was mechanically reduced from below by 10%, 20%, 30%, or 40% between January and February, 2009 (dormant season) and again between July and August, 2010 (growing season), with each harvest season and level of basal area reduction combination randomly applied to three plots. For each stump, we recorded: (1) presence of live sprouts (yes/no); (2) height (m) of the dominant (i.e., tallest) sprout, and (3) area (m(2)) occupied by individual sprout clumps. All measurements were conducted one, two, and three years post-harvest. We used logistic regression and ANOVA to analyze the probability that a stump sprouts one year post-harvest and annual stump survival (i.e., the presence of at least one live sprout), sprout height, and area. Probability of sprouting was independent of dbh for red maple, dogwood, sourwood, hickory spp., chestnut oak, yellow-poplar, and sweet birch. For sweet birch the probability of sprouting was affected by harvest season, with 54% and 93% of stumps producing sprouts one year following growing and dormant season harvests, respectively. For blackgum and white oak, dbh was negatively correlated with the probability of sprouting. Stump survival varied by species and year. Third year stump survival was 38% lower for oak and hickory than sourwood and 32% lower than red maple. Dominant sprout height was significantly greater for red maple and sourwood than for oak and hickory, with the greatest height achieved under the 40% reduction in basal area treatment. By year three, dominant sprout height for both red maple and sourwood was 40% greater than for oak and hickory and 58% greater than other shade-tolerant midstory species. Our results suggest planning harvests to occur during a particular point in the year with the idea it will limit sprouting and subsequent sprout growth is ineffective and should not be considered a viable means of reducing the production or growth of stump sprouts. Published by Elsevier B.V. C1 [Keyser, Tara L.] US Forest Serv, USDA, Southern Res Stn, Asheville, NC 28806 USA. [Zarnoch, Stanley J.] Clemson Univ, US Forest Serv, USDA, Southern Res Stn, Clemson, SC 29634 USA. RP Keyser, TL (reprint author), US Forest Serv, USDA, Southern Res Stn, 1577 Brevard Rd, Asheville, NC 28806 USA. EM tkeyser@fs.fed.us; szarnoch@fs.fed.us NR 48 TC 7 Z9 8 U1 1 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2014 VL 319 BP 29 EP 35 DI 10.1016/j.foreco.2014.01.045 PG 7 WC Forestry SC Forestry GA AG0HQ UT WOS:000335096900004 ER PT J AU Martin, FN Blair, JE Coffey, MD AF Martin, Frank N. Blair, Jaime E. Coffey, Michael D. TI A combined mitochondrial and nuclear multilocus phylogeny of the genus Phytophthora SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Oomycetes; Phytophthora; Phylogeny; Multispecies coalescent ID HIGH-TEMPERATURE TOLERANT; SUDDEN OAK DEATH; SEQUENCE-ANALYSIS; SP-NOV; EVOLUTIONARY; MULTIPLE; CRYPTOGEA; PATHOGEN; RAMORUM; IDENTIFICATION AB The most recent phylogenetic analysis of the genus Phytophthora was completed in 2008 (Blair et al., 2008) and utilized 8.1 kb of sequence data from seven nuclear loci. Given the large number of species that have recently been described, this study was undertaken to broaden the available information on the phylogeny of the genus. A total of 166 isolates representing 92 recognized species and 17 provisional species were analyzed, including many of the same isolates used in the nuclear multilocus study of Blair et al. (2008). Four mitochondrial genes (cox2, nad9, rps10 and secY) were sequenced with a total of 2373 bp used in the analysis; the species relationships recovered with mitochondrial data were largely consistent with those observed previously in the nuclear analysis. Combining the new mitochondria] data with the nuclear data from Blair et al. (2008) generated a dataset of 10,828 bp representing 11 loci, however resolution of basal clade relationships was still low. We therefore implemented a modified multispecies coalescent approach with a subset of the data, and recovered increased resolution and moderate to high support for clade relationships. A more detailed analysis of species from clades 2 and 8 identified an additional seven phylogenetic lineages that warrant further investigation to determine if they represent distinct species. As has been reported in other phylogenetic studies of the genus, there was no consistent correlation between phylogenetic relatedness and morphological features or ecology. Published by Elsevier Inc. C1 [Martin, Frank N.] USDA ARS, Salinas, CA 93905 USA. [Blair, Jaime E.] Franklin & Marshall Coll, Dept Biol, Lancaster, PA 17604 USA. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Martin, FN (reprint author), USDA ARS, Salinas, CA 93905 USA. EM frank.martin@ars.usda.gov FU USDA Agriculture and Food Research Initiative Plan Biosecurity Competitive [2007-55605-17835, 2008-55605-18773]; Franklin & Marshall College from the Commonwealth of Pennsylvania; Ben Franklin Technology Development Authority; Howard Hughes Medical Institute's Undergraduate Science Education Program FX The senior author would like to thank Lorien Radmer for her assistance in the collection of the sequence data. This work was supported by USDA Agriculture and Food Research Initiative Plan Biosecurity Competitive Grant Nos. 2007-55605-17835 and 2008-55605-18773. JEB acknowledges support from grants to Franklin & Marshall College from the Commonwealth of Pennsylvania, Ben Franklin Technology Development Authority, and from the Howard Hughes Medical Institute's Undergraduate Science Education Program. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 62 TC 24 Z9 25 U1 0 U2 31 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD MAY PY 2014 VL 66 BP 19 EP 32 DI 10.1016/j.fgb.2014.02.006 PG 14 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA AF8RY UT WOS:000334984500003 PM 24603058 ER PT J AU Iqbal, J Castellano, MJ Parkin, TB AF Iqbal, Javed Castellano, Michael J. Parkin, Timothy B. TI Accuracy and precision of no instrument is guaranteed: a reply to Rosenstock et al. SO GLOBAL CHANGE BIOLOGY LA English DT Letter ID SOIL; SPECTROSCOPY; FLUXES; N2O; CO2 C1 [Iqbal, Javed; Castellano, Michael J.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Parkin, Timothy B.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. RP Castellano, MJ (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM castellanomichaelj@gmail.com OI Castellano, Michael/0000-0003-1411-7931 NR 8 TC 1 Z9 1 U1 1 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAY PY 2014 VL 20 IS 5 BP 1363 EP 1365 DI 10.1111/gcb.12446 PG 3 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AE9WB UT WOS:000334361000001 PM 24151177 ER PT J AU Bell, CW Tissue, DT Loik, ME Wallenstein, MD Acosta - Martinez, V Erickson, RA Zak, JC AF Bell, Colin W. Tissue, David T. Loik, Michael E. Wallenstein, Matthew D. Acosta - Martinez, Veronica Erickson, Richard A. Zak, John C. TI Soil microbial and nutrient responses to 7years of seasonally altered precipitation in a Chihuahuan Desert grassland SO GLOBAL CHANGE BIOLOGY LA English DT Article DE Big Bend National Park; extreme climate events; precipitation manipulation; soil microbial communities; desert ecosystems ID FUNGAL FUNCTIONAL DIVERSITY; CLIMATE-CHANGE; COMMUNITY COMPOSITION; TERRESTRIAL ECOSYSTEMS; SEMIARID ECOSYSTEMS; AGRICULTURAL SOILS; MYCORRHIZAL FUNGI; ENZYME-ACTIVITIES; NORTH-AMERICA; CARBON AB Soil microbial communities in Chihuahuan Desert grasslands generally experience highly variable spatiotemporal rainfall patterns. Changes in precipitation regimes can affect belowground ecosystem processes such as decomposition and nutrient cycling by altering soil microbial community structure and function. The objective of this study was to determine if increased seasonal precipitation frequency and magnitude over a 7-year period would generate a persistent shift in microbial community characteristics and soil nutrient availability. We supplemented natural rainfall with large events (one/winter and three/summer) to simulate increased precipitation based on climate model predictions for this region. We observed a 2-year delay in microbial responses to supplemental precipitation treatments. In years 3-5, higher microbial biomass, arbuscular mycorrhizae abundance, and soil enzyme C and P acquisition activities were observed in the supplemental water plots even during extended drought periods. In years 5-7, available soil P was consistently lower in the watered plots compared to control plots. Shifts in soil P corresponded to higher fungal abundances, microbial C utilization activity, and soil pH. This study demonstrated that 25% shifts in seasonal rainfall can significantly influence soil microbial and nutrient properties, which in turn may have long-term effects on nutrient cycling and plant P uptake in this desert grassland. C1 [Bell, Colin W.; Wallenstein, Matthew D.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80524 USA. [Bell, Colin W.; Tissue, David T.; Zak, John C.] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Tissue, David T.] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. [Loik, Michael E.] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. [Acosta - Martinez, Veronica] USDA, Wind Eros & Water Conservat Unit, Lubbock, TX 79415 USA. [Erickson, Richard A.] Texas Tech Univ, Inst Environm & Human Hlth, Dept Environm Toxicol, Lubbock, TX 79409 USA. RP Bell, CW (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80524 USA. EM colin.bell@colostate.edu RI Young, Kristina/M-3069-2014; Wallenstein, Matthew/C-6441-2008; Tissue, David/H-6596-2015; OI Wallenstein, Matthew/0000-0002-6219-1442; Tissue, David/0000-0002-8497-2047; Erickson, Richard/0000-0003-4649-482X FU U.S. Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University; National Park Service; US Department of Energy National Institute for Climate Change Research grant; USGS Global Climate Change Small Watershed Project grant FX This research was supported by the U.S. Department of Energy's Office of Science (BER) through the Western Regional Center of the National Institute for Climatic Change Research at Northern Arizona University. We would also thank Joe Sirotnak and the other staff members of the National Park Service at Big Bend National Park for their invaluable support for this project. Funding for the research was provided by the National Park Service (JCZ, DTT and MEL), US Department of Energy National Institute for Climate Change Research grant (DTT, MEL), and a USGS Global Climate Change Small Watershed Project grant (JCZ). NR 116 TC 18 Z9 21 U1 18 U2 180 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD MAY PY 2014 VL 20 IS 5 BP 1657 EP 1673 DI 10.1111/gcb.12418 PG 17 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AE9WB UT WOS:000334361000023 PM 24115607 ER PT J AU Kich, JD Uthe, JJ Benavides, MV Cantao, ME Zanella, R Tuggle, CK Bearson, SMD AF Kich, Jalusa Deon Uthe, Jolita Janutenaite Benavides, Magda Vieira Cantao, Mauricio Egidio Zanella, Ricardo Tuggle, Christopher Keith Bearson, Shawn Michelle Dunkin TI TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs SO JOURNAL OF APPLIED GENETICS LA English DT Article DE Salmonella; Single nucleotide polymorphisms (SNPs); Swine; TLR4 ID ENTERICA SEROVAR TYPHIMURIUM; TOLL-LIKE RECEPTORS; SUS-SCROFA; EXPRESSION; DISEASE; SWINE; GENE; SUSCEPTIBILITY; INFECTION; CATTLE AB Toll-like receptor 4 (TLR4) is a key factor in the innate immune recognition of lipopolysaccharide (LPS) from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host's immune response to bacterial infections. The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs (n = 117) were intranasally challenged at 7 weeks of age with 1 x 10(9) CFU of S. Typhimurium chi 4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20/21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low (n = 20) and persistent (n = 20) Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms (SNPs) in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5' untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of Salmonella fecal shedding, and seven of those SNPs were also associated with a quantitative measurement of fecal shedding (P < 0.05). Using a stepwise regression process, a haplotype composed of SNPs rs80787918 and rs80907449 (P a parts per thousand currency signaEuro parts per thousand 4.0 x 10(-3)) spanning a region of 4.9 Kb was identified, thereby providing additional information of the influence of those SNPs on Salmonella fecal shedding in pigs. C1 [Kich, Jalusa Deon; Uthe, Jolita Janutenaite; Bearson, Shawn Michelle Dunkin] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50011 USA. [Kich, Jalusa Deon; Cantao, Mauricio Egidio] Embrapa Swine & Poultry, Concordia, SC, Brazil. [Uthe, Jolita Janutenaite; Tuggle, Christopher Keith] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Benavides, Magda Vieira] USDA, Embrapa LabEx USA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Zanella, Ricardo] CNPq, Embrapa Swine & Poultry BJT, Concordia, SC, Brazil. RP Kich, JD (reprint author), ARS, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50011 USA. EM jalusa.kich@embrapa.br RI Zanella, Ricardo/A-4644-2011; Benavides, M/F-6643-2015 OI Zanella, Ricardo/0000-0003-1449-6708; Benavides, M/0000-0002-0219-3163 FU USDA, ARS CRIS funds, National Pork Board [05-176]; National Research Initiative competitive grant from the USDA National Institute of Food and Agriculture Animal Genome Program [2009-35205-05192] FX We thank Jennifer Jones for technical assistance. This project was supported by USDA, ARS CRIS funds, National Pork Board grant #05-176, and by National Research Initiative competitive grant no. 2009-35205-05192 from the USDA National Institute of Food and Agriculture Animal Genome Program. NR 24 TC 4 Z9 4 U1 0 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1234-1983 EI 2190-3883 J9 J APPL GENET JI J. Appl. Genetics PD MAY PY 2014 VL 55 IS 2 BP 267 EP 271 DI 10.1007/s13353-014-0199-8 PG 5 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AF4LC UT WOS:000334682600013 PM 24566961 ER PT J AU Fornwalt, PJ Kaufmann, MR AF Fornwalt, Paula J. Kaufmann, Merrill R. TI Understorey plant community dynamics following a large, mixed severity wildfire in a Pinus ponderosa-Pseudotsuga menziesii forest, Colorado, USA SO JOURNAL OF VEGETATION SCIENCE LA English DT Article DE Riparian areas; Time since fire; Hayman Fire; Colorado Front Range; Ponderosa pine; Douglas-fir; Fire severity ID YELLOWSTONE-NATIONAL-PARK; FIRE SEVERITY; MEDITERRANEAN BASIN; VEGETATION RESPONSE; TREE REGENERATION; NORTHERN ARIZONA; PRESCRIBED FIRE; FRONT RANGE; DIVERSITY; LANDSCAPES AB Question How do understorey plant communities of Pinus ponderosa-Pseudotsuga menziesii forests respond during the first 5yrs following wildfire, and do responses vary with fire severity? Location Colorado Front Range, USA. Methods In 2002, the Hayman Fire burned across 55800ha of Colorado Front Range P.ponderosa-P.menziesii forest. Also burned in the fire were 20 upland and five riparian plots within a 400-ha study area. These plots had been surveyed for understorey plant composition and cover 5-6yrs prior. We re-measured all plots annually from 2003 to 2007, 1-5yrs post-fire. Changes in the occurrence of common understorey plant species and in metrics of understorey plant richness and cover were analysed with regard to fire severity and time since fire using repeated measures ANOVA. Compositional changes were explored using ordination. Results Fire severity (defined in terms of overstorey mortality, overstorey canopy consumption, and forest floor consumption) in upland plots was highly variable, with 50%, 30% and 20% of plots burning with low, moderate and high severity, respectively. For all severities, total cover in uplands declined in the first post-fire year relative to pre-fire levels, but met or exceeded pre-fire levels by post-fire year five. Total richness in uplands, however, did not similarly decline immediately following fire, due largely to a high return rate of pre-fire species, and exceeded pre-fire levels for all severities from post-fire years 3-5 due to new species recruitment. Over 90% of the common upland species either were found in a similar number of plots before and after the fire, regardless of fire severity or time since fire, or were found in more plots following the fire in at least 1yr and one severity class. Temporal changes in upland composition occurred for all severities but were most pronounced following moderate and severe fire. In contrast, riparian plots largely burned with low severity, and the understorey plant communities within them exhibited little change in richness, cover and composition over the 5yrs. Conclusions Our results suggest that the Hayman Fire had largely neutral or stimulatory impacts on understorey plant communities following the first five post-fire years. C1 [Fornwalt, Paula J.; Kaufmann, Merrill R.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Fornwalt, PJ (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80526 USA. EM pfornwalt@fs.fed.us; mkauf@lamar.colostate.edu FU Joint Fire Science Program [03-2-3-08, 04-2-1-118]; USDA Forest Service Rocky Mountain Research Station FX This research project was funded by the Joint Fire Science Program (Project Numbers 03-2-3-08 and 04-2-1-118) and the USDA Forest Service Rocky Mountain Research Station. We gratefully acknowledge Stephanie Asherin, Allison Grow, Rebecca Hemmerling, Micky McNaughton, Jill Oropeza, Lisa Schell, Rick Shory, Betsy Smith and Jennifer Ventker for their assistance with botanical surveys, data management and specimen archiving. We thank Scott Abella, Laurie Huckaby, William Romme, John Frank and three anonymous reviewers for insightful comments on the manuscript. We also thank Rudy King, Scott Baggett and Jim zumBrunnen for statistical advice, and Benjamin Bird and John Frank for help with figure preparation. Lodging, lab and office facilities during field campaigns were provided by the Manitou Experimental Forest. NR 59 TC 5 Z9 5 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1100-9233 EI 1654-1103 J9 J VEG SCI JI J. Veg. Sci. PD MAY PY 2014 VL 25 IS 3 BP 805 EP 818 DI 10.1111/jvs.12128 PG 14 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA AE7NH UT WOS:000334184300019 ER PT J AU Li, RG Ling, KS AF Li, Rugang Ling, Kai-Shu TI Development of reverse transcription loop-mediated isothermal amplification assay for rapid detection of an emerging potyvirus: Tomato necrotic stunt virus SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Solanum lycopersicum; qRT-PCR; LAMP assay; Field disease diagnosis ID LEAF CURL VIRUS; MOSAIC-VIRUS; RT-PCR; PLANTS; LAMP; DNA AB Tomato necrotic stunt virus (ToNStV) is an emerging potyvirus that causes severe stunting to infected tomato plants. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for sensitive detection of ToNStV. The sensitivity of RT-LAMP was comparable to that of conventional RT-PCR, with detection of ToNStV in a reaction containing only 8 pg of total tomato RNA or with 1:20,000 dilution of crude tissue extract. This assay was able to detect ToNStV in a broad range of solanaceous plant species. The RT-LAMP for ToNStV was specific with no cross-reactivity to other potyviruses (i.e. Potato virus Y and Tobacco etch virus), as well as several other common tomato viruses. RT-LAMP should complement RT-PCR and real-time RT-PCR assays reported previously, with a potential to provide a simple, rapid, and sensitive field diagnostic method for ToNStV. Published by Elsevier B.V. C1 [Li, Rugang; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Rugang.Li@ars.usda.gov; Kai.Ling@ars.usda.gov FU USDA, National Institute of Food and Agriculture's Specialty Crop Research Initiative programs [SCRI 2010-600-25320, SCRI 2012-01507-229756] FX We thank Dr. W. Patrick Wechter for his critical reading of the manuscript and Andrea Gilliard, Alan Wilder, Mindie Lipphardt and Emily Fillippeli for their excellent technical assistance. This work was supported in part by USDA, National Institute of Food and Agriculture's Specialty Crop Research Initiative programs SCRI 2010-600-25320 and SCRI 2012-01507-229756 to KSL. NR 19 TC 4 Z9 4 U1 0 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD MAY PY 2014 VL 200 BP 35 EP 40 DI 10.1016/j.jviromet.2014.01.017 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA AF8QX UT WOS:000334981800007 PM 24503040 ER PT J AU Lou, Y Clay, SA Davis, AS Dille, A Felix, J Ramirez, AHM Sprague, CL Yannarell, AC AF Lou, Yi Clay, Sharon A. Davis, Adam S. Dille, Anita Felix, Joel Ramirez, Analiza H. M. Sprague, Christy L. Yannarell, Anthony C. TI An Affinity-Effect Relationship for Microbial Communities in Plant-Soil Feedback Loops SO MICROBIAL ECOLOGY LA English DT Article ID ARBUSCULAR MYCORRHIZAL FUNGI; INTERGENIC SPACER ANALYSIS; BELOW-GROUND ENEMIES; ARABIDOPSIS-THALIANA; BACTERIAL COMMUNITIES; RHIZOSPHERE BACTERIA; BIOTA INTERACTIONS; DIVERSITY; COEXISTENCE; PATHOGENS AB Feedback loops involving soil microorganisms can regulate plant populations. Here, we hypothesize that microorganisms are most likely to play a role in plant-soil feedback loops when they possess an affinity for a particular plant and the capacity to consistently affect the growth of that plant for good or ill. We characterized microbial communities using whole-community DNA fingerprinting from multiple "home-and-away" experiments involving giant ragweed (Ambrosia trifida L.) and common sunflower (Helianthus annuus L.), and we looked for affinity-effect relationships in these microbial communities. Using canonical ordination and partial least squares regression, we developed indices expressing each microorganism's affinity for ragweed or sunflower and its putative effect on plant biomass, and we used linear regression to analyze the relationship between microbial affinity and effect. Significant linear affinity-effect relationships were found in 75 % of cases. Affinity-effect relationships were stronger for ragweed than for sunflower, and ragweed affinity-effect relationships showed consistent potential for negative feedback loops. The ragweed feedback relationships indicated the potential involvement of multiple microbial taxa, resulting in strong, consistent affinity-effect relationships in spite of large-scale microbial variability between trials. In contrast, sunflower plant-soil feedback may involve just a few key players, making it more sensitive to underlying microbial variation. We propose that affinity-effect relationship can be used to determine key microbial players in plant-soil feedback against a low "signal-to-noise" background of complex microbial datasets. C1 [Lou, Yi; Yannarell, Anthony C.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Clay, Sharon A.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Davis, Adam S.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL USA. [Dille, Anita; Ramirez, Analiza H. M.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Felix, Joel] Oregon State Univ, Dept Crop & Soil Sci, Corvalis, OR USA. [Sprague, Christy L.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Yannarell, AC (reprint author), Univ Illinois, Dept Nat Resources & Environm Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM acyann@illinois.edu FU USDA-CSREES [ILLU-875-317, NC1026] FX The authors acknowledge A. Kent, D. Keymer, J. Koval, D. Li, H.-M. Lin, S. Paver, and A. Peralta for valuable feedback during the preparation of this manuscript. This research was supported by the USDA-CSREES (Project ILLU-875-317 and Multistate Research Project NC1026). NR 58 TC 1 Z9 1 U1 4 U2 57 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 EI 1432-184X J9 MICROB ECOL JI Microb. Ecol. PD MAY PY 2014 VL 67 IS 4 BP 866 EP 876 DI 10.1007/s00248-013-0349-2 PG 11 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA AF1SX UT WOS:000334495000014 PM 24402363 ER PT J AU La Frano, MR de Moura, FF Boy, E Lonnerdal, B Burri, BJ AF La Frano, Michael R. de Moura, Fabiana F. Boy, Erick Loennerdal, Bo Burri, Betty J. TI Bioavailability of iron, zinc, and provitamin A carotenoids in biofortified staple crops SO NUTRITION REVIEWS LA English DT Review DE zinc; vitamin A; bioavailability; iron; biofortified ID BEANS PHASEOLUS-VULGARIS; VITAMIN-A-DEFICIENCY; IN-VITRO DIGESTION; GERBILS MERIONES-UNGUICULATUS; TRACE-ELEMENT BIOAVAILABILITY; RICH LIPOPROTEIN FRACTION; HUMAN INTESTINAL-CELLS; POULTRY GALLUS-GALLUS; FLESHED SWEET-POTATO; TRANS BETA-CAROTENE AB International research efforts, including those funded by HarvestPlus, a Challenge Program of the Consultative Group on International Agricultural Research (CGIAR), are focusing on conventional plant breeding to biofortify staple crops such as maize, rice, cassava, beans, wheat, sweet potatoes, and pearl millet to increase the concentrations of micronutrients that are commonly deficient in specific population groups of developing countries. The bioavailability of micronutrients in unfortified staple crops in developing regions is typically low, which raises questions about the efficacy of these crops to improve population micronutrient status. This review of recent studies of biofortified crops aims to assess the micronutrient bioavailability of biofortified staple crops in order to derive lessons that may help direct plant breeding and to infer the potential efficacy of food-based nutrition interventions. Although reducing the amounts of antinutrients and the conduction of food processing generally increases the bioavailability of micronutrients, antinutrients still possess important benefits, and food processing results in micronutrient loss. In general, biofortified foods with relatively higher micronutrient density have higher total absorption rates than nonbiofortified varieties. Thus, evidence supports the focus on efforts to breed plants with increased micronutrient concentrations in order to decrease the influence of inhibitors and to offset losses from processing. C1 [La Frano, Michael R.; Loennerdal, Bo; Burri, Betty J.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [La Frano, Michael R.; Burri, Betty J.] USDA, Western Human Nutr Res Ctr, Davis, CA USA. [de Moura, Fabiana F.; Boy, Erick] Int Food Policy Res Inst, HarvestPlus, Washington, DC 20036 USA. RP La Frano, MR (reprint author), 3135 Meyer Hall,One Shields Ave, Davis, CA 95616 USA. EM mrlafrano@ucdavis.edu OI De Moura, Fabiana F./0000-0001-8176-5352; La Frano, Michael/0000-0002-2269-1688 FU HarvestPlus, International Food Policy Research Institute (IFPRI) [2013X254FRA] FX This review was funded through HarvestPlus, c/o the International Food Policy Research Institute (IFPRI), contract no. 2013X254FRA. NR 148 TC 24 Z9 24 U1 14 U2 73 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD MAY PY 2014 VL 72 IS 5 BP 289 EP 307 DI 10.1111/nure.12108 PG 19 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF7OK UT WOS:000334904200001 PM 24689451 ER PT J AU Shi, ZQ Balogh-Brunstad, Z Grant, M Harsh, J Gill, R Thomashow, L Dohnalkova, A Stacks, D Letourneau, M Keller, CK AF Shi, Zhenqing Balogh-Brunstad, Zsuzsanna Grant, Michael Harsh, James Gill, Richard Thomashow, Linda Dohnalkova, Alice Stacks, Daryl Letourneau, Melissa Keller, C. Kent TI Cation uptake and allocation by red pine seedlings under cation-nutrient stress in a column growth experiment SO PLANT AND SOIL LA English DT Article DE Cation-nutrient stress; Environmental tracer; Plant fractionation; Discrimination factor; Mineral weathering ID FOLIAR CA/SR DISCRIMINATION; FOREST ECOSYSTEMS; WEATHERING RATES; POTASSIUM UPTAKE; RELATIVE UPTAKE; HUBBARD BROOK; UNITED-STATES; CA SOURCES; CALCIUM; PLANTS AB Plant nutrient uptake is affected by environmental stress, but how plants respond to cation-nutrient stress is poorly understood. We assessed the impact of varying degrees of cation-nutrient stress on cation uptake in an experimental plant-mineral system. Column experiments, with red pine (Pinus resinosa Ait.) seedlings growing in sand/mineral mixtures, were conducted for up to 9 months. The Ca and K were supplied from both minerals and nutrient solutions with varying Ca and K concentrations. Cation nutrient stress had little impact on carbon allocation after 9 months of plant growth and K was the limiting nutrient for biomass production. Measurement of Ca/Sr and K/Rb ratios allowed independent estimation of dissolution incongruency and discrimination against Sr and Rb during cation uptake processes. The fraction of K in biomass from biotite increased with decreasing K supply from nutrient solutions. The mineral anorthite was consistently the major source of Ca, regardless of nutrient treatment. Red pine seedlings exploited more mineral K in response to more severe K deficiency. This did not occur for Ca since Ca was not limiting plant growth. Plant discrimination factors must be carefully considered to accurately identify nutrient sources using cation tracers. C1 [Shi, Zhenqing; Stacks, Daryl; Keller, C. Kent] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. [Shi, Zhenqing; Grant, Michael; Harsh, James; Letourneau, Melissa] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Balogh-Brunstad, Zsuzsanna] Hartwick Coll, Dept Chem, Oneonta, NY 13820 USA. [Balogh-Brunstad, Zsuzsanna] Hartwick Coll, Dept Geol & Environm Sci, Oneonta, NY 13820 USA. [Gill, Richard] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Thomashow, Linda] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Dohnalkova, Alice] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA. RP Shi, ZQ (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM zhenqing.shi@wsu.edu RI Shi, Zhenqing /F-9212-2016; Harsh, James/C-7455-2014 OI Harsh, James/0000-0002-0177-3342 FU National Science Foundation [0952399]; U.S. DOE's Office of Biological and Environmental Research (OBER), located at the Pacific Northwest National Laboratory (PNNL); DOE [DE-AC06-76RLO 1830] FX We thank Jeff Boyle, Scott Boroughs, and Charles Knaack at Washington State University for analytical assistance and helpful discussions. We thank our collaborators at Washington State University, particularly generous and ongoing accommodations within the School of Biological Sciences Plant Growth Service Center. This work is supported by National Science Foundation grant No. 0952399 to Kent Keller and collaborators. A portion of this research was performed at the Environmental Molecular Sciences laboratory (EMSL), a national scientific user facility sponsored by the U.S. DOE's Office of Biological and Environmental Research (OBER), located at the Pacific Northwest National Laboratory (PNNL). PNNL is operated for the DOE by Battelle Memorial Institute under contract DE-AC06-76RLO 1830. NR 41 TC 0 Z9 1 U1 1 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD MAY PY 2014 VL 378 IS 1-2 BP 83 EP 98 DI 10.1007/s11104-013-2016-2 PG 16 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AF2AC UT WOS:000334514000006 ER PT J AU Butler, DM Kokalis-Burelle, N Albano, JP McCollum, TG Muramoto, J Shennan, C Rosskopf, EN AF Butler, David M. Kokalis-Burelle, Nancy Albano, Joseph P. McCollum, T. Greg Muramoto, Joji Shennan, Carol Rosskopf, Erin N. TI Anaerobic Soil Disinfestation (ASD) Combined with Soil Solarization as a Methyl Bromide Alternative: Vegetable Crop Performance and Soil Nutrient Dynamics SO PLANT AND SOIL LA English DT Article DE Soil disinfestation; Methyl bromide alternatives; Molasses; Organic amendments; Bell pepper; Eggplant; Solarization; Vegetable nutrition ID AMMONIA-OXIDIZING BACTERIA; SOILBORNE PLANT-PATHOGENS; ORGANIC AMENDMENTS; MICROBIAL COMMUNITIES; POULTRY LITTER; BSD; NITRIFICATION; STERILIZATION; MECHANISMS; MANAGEMENT AB Soil treatment by anaerobic soil disinfestation (ASD) combined with soil solarization can effectively control soilborne plant pathogens and plant-parasitic nematodes in specialty crop production systems. At the same time, research is limited on the impact of soil treatment by ASD + solarization on soil fertility, crop performance and plant nutrition. Our objectives were to evaluate the response of 1) soil nutrients and 2) vegetable crop performance to ASD + solarization with differing levels of irrigation, molasses amendment, and partially-composted poultry litter amendment (CPL) compared to an untreated control and a methyl bromide (MeBr) + chloropicrin-fumigated control. A 2-year field study was established in 2008 at the USDA-ARS U.S. Horticultural Research Lab in Fort Pierce, Florida, USA to determine the effectiveness of ASD as an alternative to MeBr fumigation for a bell pepper (Capsicum annum L.)-eggplant (Solanum melongena L.) double crop system. A complete factorial combination of treatments in a split-split plot was established to evaluate three levels of initial irrigation [10, 5, or 0 cm], two levels of CPL (amended or unamended), and two levels of molasses (amended or unamended) in combination with solarization. Untreated and MeBr controls were established for comparison to ASD treatments. Results suggest that ASD treatment using molasses as the carbon source paired with solarization can be an effective strategy to maintain crop yields in the absence of soil fumigants. For both bell pepper and eggplant crops, ASD treatments with molasses as the carbon source had equivalent or greater marketable yields than the MeBr control. The application of organic amendments in ASD treatment (molasses or molasses + CPL) caused differences in soil nutrients and plant nutrition compared to the MeBr control that must be effectively managed in order to implement ASD on a commercial scale as a MeBr replacement. C1 [Butler, David M.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Kokalis-Burelle, Nancy; Albano, Joseph P.; McCollum, T. Greg; Rosskopf, Erin N.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Muramoto, Joji; Shennan, Carol] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. RP Butler, DM (reprint author), Univ Tennessee, Dept Plant Sci, 2431 Joe Johnson Dr, Knoxville, TN 37996 USA. EM dbutler@utk.edu RI Shennan, Carol/I-1694-2013 OI Shennan, Carol/0000-0001-6401-5007 FU USDA-NIFA Methyl Bromide Transitions [2007-51102-03854, 2010-51102-21707] FX The authors wish to thank Kate Rotindo, Melissa Edgerly, Bernardette Stange, Amanda Rinehart, John Mulvaney, Jackie Markle, Randy Driggers, Gene Swearingen, Don Beauchaine, Steve Mayo, Veronica Abel, William Crawford, James Salvatore, Wayne Brown, Chris Lasser, and Pragna Patel for their assistance with the field and laboratory work. Funding for a portion of this work was provided by the USDA-NIFA Methyl Bromide Transitions Grant Agreements 2007-51102-03854 and 2010-51102-21707. The authors wish to thank Seminis Vegetable Seeds, Inc., Saint Louis, Missouri, USA for the donation of vegetable seeds and Johnson Plants Inc., Immokalee, FL, USA for assistance with transplant production. NR 43 TC 11 Z9 14 U1 9 U2 60 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD MAY PY 2014 VL 378 IS 1-2 BP 365 EP 381 DI 10.1007/s11104-014-2030-z PG 17 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AF2AC UT WOS:000334514000026 ER PT J AU Berger, G Green, A Khatibi, P Brooks, W Rosso, L Liu, SY Chao, SM Griffey, C Schmale, D AF Berger, Gregory Green, Andrew Khatibi, Piyum Brooks, Wynse Rosso, Luciana Liu, Shuyu Chao, Shiaoman Griffey, Carl Schmale, David, III TI Characterization of Fusarium Head Blight Resistance and Deoxynivalenol Accumulation in Hulled and Hulless Winter Barley SO PLANT DISEASE LA English DT Article ID QUANTITATIVE TRAIT LOCI; KERNEL DISCOLORATION; QTL ANALYSIS; REGISTRATION; POPULATION; WHEAT; GRAIN; IDENTIFICATION; VALIDATION; CROSS AB Fusarium head blight (FHB), caused by Fusariuin graminearum, is one of the most serious diseases impacting the U.S. barley (Hordeum vulgare) industry. The mycotoxin deoxynivalenol (DON), produced by the pathogen, renders grain unmarketable if concentrations exceed threshold values set for end-use markets. Development of cultivars with improved FHB resistance and reduced DON accumulation is necessary to ensure minimal losses. Elite hulled and hulless genotypes developed by the Virginia Tech winter barley breeding program were screened in inoculated, mist-irrigated FHB nurseries over 2 years at two locations in Virginia to validate resistance levels over years and locations. Results demonstrated that barley genotypes varied significantly for resistance to FHB and DON accumulation. The hulled 'Nomini', hulless 'Eve', and hulless line VA06H-48 were consistently resistant across locations to both FHB and DON accumulation. Screening the genotypes with molecular markers on chromosomes 2H and 6H for FHB and DON revealed quantitative trait loci regions which may confer resistance in the Virginia Tech germplasm. Ongoing and future work with mapping populations seeks to identify novel regions for resistance to FHB and DON accumulation unique to the Virginia Tech breeding program. C1 [Berger, Gregory] Univ Arkansas, Rice Res & Extens Ctr, Dept Crop Soil & Environm Sci, D-72160 Stuttgart, Germany. [Green, Andrew] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Khatibi, Piyum; Schmale, David, III] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Brooks, Wynse; Rosso, Luciana; Griffey, Carl] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Liu, Shuyu] Texas A&M, Texas AgriLife Res, Amarillo, TX 79106 USA. [Chao, Shiaoman] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. RP Berger, G (reprint author), Univ Arkansas, Rice Res & Extens Ctr, Dept Crop Soil & Environm Sci, D-72160 Stuttgart, Germany. EM glberger@uark.edu; cgriffey@vt.edu; dschmale@vt.edu OI Schmale, David/0000-0002-7003-7429 FU Virginia Small Grains Board [10278306]; U.S. Wheat and Barley Scab Initiative [07185403]; USWBSI [59-0790-4-102] FX This work was supported, in part, by grants to D. Schmale from the Virginia Small Grains Board (10278306) and the U.S. Wheat and Barley Scab Initiative (07185403), and to C. A. Griffey from the USWBSI (59-0790-4-102). We thank N. McMaster for her technical assistance in analyzing grain samples for DON and R. Horsley for access to his SNP marker set. NR 45 TC 3 Z9 3 U1 6 U2 32 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 599 EP 606 DI 10.1094/PDIS-05-13-0479-RE PG 8 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800003 ER PT J AU Nyczepir, AP Brannen, PM Cook, J Meyer, SLF AF Nyczepir, A. P. Brannen, P. M. Cook, J. Meyer, S. L. F. TI Management of Meloidogyne incognita with Jesup (Max-Q) Tall Fescue Grass Prior to Peach Orchard Establishment SO PLANT DISEASE LA English DT Article ID MESOCRICONEMA-XENOPLAX; SHORT LIFE; ENDOPHYTE; TREES; SOIL; REPRODUCTION; BAHIAGRASS; GROWTH AB The effects of two preplant Jesup (Max-Q) tall fescue (Schedonorus arundinaceus) ground cover treatments as alternatives to chemical control of Meloidogyne incognita were investigated from 2006 to 2013. The experiment was initiated in 2006 in a site known to be infested with M. incognita. Treatments included (i) 1 year of peach followed by 1 year of Jesup (Max-Q), (ii) 2 years of continuous Jesup (Max-Q), (iii) 2 years of continuous peach, and (iv) 2 years of continuous peach followed by fumigation with 1,3-dichloropropene (1,3-D). Both Jesup (Max-Q) treatments suppressed (P <= 0.05) population densities of M. incognita second-stage juveniles (J2) compared with the 2-year continuous peach treatments (prior to fumigation); no J2 were detected in soil samples taken from 2-year continuous Jesup (Max-Q) plots. Evaluation of the effects of the Jesup (Max-Q) treatments on subsequent peach tree planting was initiated in 2008, when herbicide was applied to the tall fescue treatment plots and half the continuous peach plots were fumigated with 1,3-D (nematicide treatment control). Peach trees were planted into all plots in 2009. By the end of the experiment (48 months after orchard establishment), trunk diameter was greater (P <= 0.01) in both of the Jesup (Max-Q) treatments and in the 1,3-D-fumigated treatment than in the nonfumigated treatment. Moreover, trunk diameter did not differ among the Jesup (Max-Q) and the fumigated treatments. Preplant Jesup (Max-Q) tall fescue was as effective as 1,3-D fumigation in increasing tree growth in an M. incognita-infested site. C1 [Nyczepir, A. P.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Brannen, P. M.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Cook, J.] Univ Georgia, Cooperat Extens Serv, Butler, GA 31006 USA. [Meyer, S. L. F.] USDA ARS, Nematol Lab, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Nyczepir, AP (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM nyczepir@windstream.net NR 32 TC 0 Z9 0 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 625 EP 630 DI 10.1094/PDIS-08-13-0849-RE PG 6 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800006 ER PT J AU Weiland, JE AF Weiland, Jerry E. TI Pythium Species and Isolate Diversity Influence Inhibition by the Biological Control Agent Streptomyces lydicus SO PLANT DISEASE LA English DT Article ID DOUGLAS-FIR SEEDLINGS; FUSARIUM ROOT DISEASE; DAMPING-OFF; BIOCONTROL AGENTS; SOILBORNE PATHOGENS; FOREST NURSERIES; FUNGAL ROOT; PSEUDOMONAS; SEED; SOIL AB Disease control of soilborne pathogens by biological control agents (BCAs) is often inconsistent under field conditions. This inconsistency may be partly influenced by pathogen diversity if there is a differential response among pathogen species and isolates to selected BCAs. The responses of 148 Pythium isolates obtained from soil at three forest nurseries and representative of 16 Pythium spp. were evaluated in the presence of Streptomyces lydicus strain WYEC108 in an in vitro assay. Percent growth inhibition, inhibition zone distance, mortality, and growth rate were recorded for each isolate, and data were analyzed for effects of species and isolate. Responses of three Pythium spp. (Pythium irregulare, P sylvaticum, and P. ultimum) were further analyzed for a location (nursery) effect. Although S. lydicus inhibited all Pythium isolates, differences in percent growth inhibition, inhibition zone distance, and mortality were observed among Pythium spp. and isolates. Small but significant location effects were also noted. Growth rate also varied among Pythium spp. and isolates and was found to strongly bias percent growth inhibition and, to a lesser degree, inhibition zone distance; depending on which measure was used, slower-growing isolates appeared less sensitive (growth inhibition) or more sensitive (inhibition zone) to S. lydicus than faster-growing isolates. Results illustrate the importance of using multiple, representative pathogen isolates in preliminary BCA inhibition assays as well as accounting for the effect of pathogen growth rate on pathogen inhibition by BCAs. Future studies should take pathogen diversity into account when evaluating biological control efficacy. C1 [Weiland, Jerry E.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. [Weiland, Jerry E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. EM Jerry.Weiland@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service CRIS [5358-12220-004-00]; Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives FX Financial support was provided, in part, by funds from the United States Department of Agriculture-Agricultural Research Service CRIS Project 5358-12220-004-00, and grants from the Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives. I thank A. Davis, B. Beck, and D. Kroese for technical assistance; V. Fieland and the Nik Grunwald lab for assistance in the identification of Pythium isolates; and C. Scagel for assistance with statistical analyses. NR 50 TC 3 Z9 3 U1 1 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 653 EP 659 DI 10.1094/PDIS-05-13-0482-RE PG 7 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800009 ER PT J AU du Toit, LJ Derie, ML Christianson, CE Hoagland, L Simon, P AF du Toit, L. J. Derie, M. L. Christianson, C. E. Hoagland, L. Simon, P. TI First Report of Bacterial Blight of Carrot in Indiana Caused by Xanthomonas hortorum pv. carotae SO PLANT DISEASE LA English DT News Item C1 [du Toit, L. J.; Derie, M. L.; Christianson, C. E.] Washington State Univ, NWREC, Mt Vernon, WA 98273 USA. [Hoagland, L.] Purdue Univ, W Lafayette, IN 47907 USA. [Simon, P.] USDA ARS, Madison, WI 53706 USA. RP du Toit, LJ (reprint author), Washington State Univ, NWREC, Mt Vernon, WA 98273 USA. NR 5 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 685 EP 685 DI 10.1094/PDIS-10-13-1083-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800021 ER PT J AU Jurick, WM Kou, LP Gaskins, VL Luo, YG AF Jurick, W. M., II Kou, L. P. Gaskins, V. L. Luo, Y. G. TI First Report of Alternaria alternata Causing Postharvest Decay on Apple Fruit During Cold Storage in Pennsylvania SO PLANT DISEASE LA English DT News Item ID IDENTIFICATION C1 [Jurick, W. M., II; Gaskins, V. L.; Luo, Y. G.] USDA ARS, Food Qual Lab, Beltsville, MD USA. [Kou, L. P.] Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. RP Jurick, WM (reprint author), USDA ARS, Food Qual Lab, Beltsville, MD USA. NR 14 TC 1 Z9 1 U1 2 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 690 EP 691 DI 10.1094/PDIS-08-13-0817-PDN PG 2 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800035 ER PT J AU Kou, LP Gaskins, VL Luo, YG Jurick, WM AF Kou, L. P. Gaskins, V. L. Luo, Y. G. Jurick, W. M., II TI First Report of Alternaria tenuissima Causing Postharvest Decay on Apple Fruit from Cold Storage in the United States SO PLANT DISEASE LA English DT News Item C1 [Kou, L. P.] Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. [Gaskins, V. L.; Luo, Y. G.; Jurick, W. M., II] USDA ARS, Food Qual Lab, Beltsville, MD USA. RP Kou, LP (reprint author), Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. NR 3 TC 3 Z9 3 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 690 EP 690 DI 10.1094/PDIS-07-13-0802-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800033 ER PT J AU Crouch, JA Ko, MP McKemy, JM AF Crouch, J. A. Ko, M. P. McKemy, J. M. TI First Report of Impatiens Downy Mildew Outbreaks Caused by Plasmopara obducens Throughout the Hawai'ian Islands SO PLANT DISEASE LA English DT News Item C1 [Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Ko, M. P.] Hawaii Dept Agr, Honolulu, HI 96814 USA. [McKemy, J. M.] USDA APHIS PPQ Natl Identificat Serv, Beltsville, MD 20705 USA. RP Crouch, JA (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. OI Crouch, Jo Anne/0000-0001-6886-8090 NR 6 TC 2 Z9 2 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 696 EP 696 DI 10.1094/PDIS-10-13-1017-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800048 ER PT J AU Malapi-Wight, M Hebert, JB Buckley, R Daughtrey, ML Gregory, NF Rane, K Tirpak, S Crouch, JA AF Malapi-Wight, M. Hebert, J. B. Buckley, R. Daughtrey, M. L. Gregory, N. F. Rane, K. Tirpak, S. Crouch, J. A. TI First Report of Boxwood Blight Caused by Calonectria pseudonaviculata in Delaware, Maryland, New Jersey, and New York. SO PLANT DISEASE LA English DT News Item ID CYLINDROCLADIUM-PSEUDONAVICULATUM C1 [Malapi-Wight, M.; Hebert, J. B.; Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Malapi-Wight, M.; Hebert, J. B.; Buckley, R.; Tirpak, S.] Rutgers State Univ, Dept Plant Biol, New Brunswick, NJ 08901 USA. [Daughtrey, M. L.] Cornell Univ, Long Isl Hort Res & Extens Ctr, Riverhead, NY 11901 USA. [Gregory, N. F.] Univ Delaware, Newark, DE 19716 USA. [Rane, K.] Univ Maryland, Plant Diagnost Lab, College Pk, MD 20742 USA. RP Malapi-Wight, M (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. OI Crouch, Jo Anne/0000-0001-6886-8090; Malapi-Wight, Martha/0000-0003-2249-6257 NR 4 TC 6 Z9 6 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 698 EP 698 DI 10.1094/PDIS-10-13-1102-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800053 ER PT J AU Ling, KS Li, R Zhang, W AF Ling, K. -S. Li, R. Zhang, W. TI First Report of Cucumber green mottle mosaic virus Infecting Green-house Cucumber in Canada. SO PLANT DISEASE LA English DT News Item C1 [Ling, K. -S.; Li, R.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Zhang, W.] Alberta Agr & Rural Dev, Greenhouse Branch, Crop Res & Extens Div, Brooks, AB, Canada. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. NR 3 TC 5 Z9 5 U1 0 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 701 EP 701 DI 10.1094/PDIS-09-13-0996-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800061 ER PT J AU Ling, KS Li, R Groth-Helms, D Assis, FM AF Ling, K. -S. Li, R. Groth-Helms, D. Assis-Filho, F. M. TI First Report of Potato spindle tuber viroid Naturally Infecting Field Tomatoes in the Dominican Republic. SO PLANT DISEASE LA English DT News Item ID GREENHOUSE TOMATOES C1 [Ling, K. -S.; Li, R.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Groth-Helms, D.; Assis-Filho, F. M.] Agdia Inc, Elkhart, IN 46514 USA. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. NR 4 TC 5 Z9 5 U1 0 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2014 VL 98 IS 5 BP 701 EP 701 DI 10.1094/PDIS-09-13-0992-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AF2VE UT WOS:000334569800060 ER PT J AU Milner, MJ Mitani-Ueno, N Yamaji, N Yokosho, K Craft, E Fei, ZJ Ebbs, S Zambrano, MC Ma, JF Kochian, LV AF Milner, Matthew J. Mitani-Ueno, Namiki Yamaji, Naoki Yokosho, Kengo Craft, Eric Fei, Zhangjun Ebbs, Stephen Zambrano, M. Clemencia Ma, Jian Feng Kochian, Leon V. TI Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation SO PLANT JOURNAL LA English DT Article DE cadmium; Nramp; Cd hyperaccumulation; hyperaccumulation; Noccaea caerulescens; Cd transport ID METAL TRANSPORTER GENES; THLASPI-CAERULESCENS; ARABIDOPSIS-THALIANA; CONTRASTING ECOTYPES; MOLECULAR PHYSIOLOGY; ELEVATED EXPRESSION; ZN HYPERACCUMULATOR; ZINC ACCUMULATION; PROBE LEVEL; CADMIUM AB The Zn/Cd hyperaccumulator, Noccaea caerulescens, has been studied extensively for its ability to accumulate high levels of Zn and Cd in its leaves. Previous studies have indicated that the Zn and Cd hyperaccumulation trait exhibited by this species involves different transport and tolerance mechanisms. It has also been well documented that certain ecotypes of N.caerulescens are much better Cd hyperaccumulators than others. However, there does not seem to be much ecotypic variation for Zn hyperaccumulation in N.caerulescens. In this study we employed a comparative transcriptomics approach to look at root and shoot gene expression in Ganges and Prayon plants in response to Cd stress to identify transporter genes that were more highly expressed in either the roots or shoots of the superior Cd accumulator, Ganges. Comparison of the transcriptomes from the two ecotypes of Noccaea caerulescens identified a number of genes that encoded metal transporters that were more highly expressed in the Ganges ecotype in response to Cd stress. Characterization of one of these transporters, NcNramp1, showed that it is involved in the influx of Cd across the endodermal plasma membrane and thus may play a key role in Cd flux into the stele and root-to-shoot Cd transport. NcNramp1 may be one of the main transporters involved in Cd hyperaccumulation in N.caerulescens and copy number variation appears to be the main reason for high NcNramp1 gene expression underlying the increased Cd accumulation in the Ganges ecotype. C1 [Milner, Matthew J.; Craft, Eric; Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Milner, Matthew J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Mitani-Ueno, Namiki; Yamaji, Naoki; Yokosho, Kengo; Ma, Jian Feng] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst, Ithaca, NY 14853 USA. [Fei, Zhangjun] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. [Ebbs, Stephen; Zambrano, M. Clemencia] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA. RP Kochian, LV (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM maj@rib.okayama-u.ac.jp; leon.kochian@ars.usda.gov RI YAMAJI, Naoki/B-2524-2011; Ma, Jian Feng/C-7213-2009; OI YAMAJI, Naoki/0000-0002-7499-3004; Kochian, Leon/0000-0003-3416-089X; Ebbs, Stephen/0000-0003-4042-9234 FU United States Department of Agriculture (USDA) Agricultural Research Service; Ministry of Education, Culture, Sports, Science and Technology of Japan [22119002, 24248014] FX The research was supported by funding from the United States Department of Agriculture (USDA) Agricultural Research Service (to LV.K.) and a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan (nos. 22119002 and 24248014 to J.F.M.). NR 43 TC 16 Z9 17 U1 3 U2 67 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD MAY PY 2014 VL 78 IS 3 BP 398 EP 410 DI 10.1111/tpj.12480 PG 13 WC Plant Sciences SC Plant Sciences GA AF6KP UT WOS:000334824300004 PM 24547775 ER PT J AU Nuutinen, V Butt, KR Jauhiainen, L Shipitalo, MJ Siren, T AF Nuutinen, Visa Butt, Kevin R. Jauhiainen, Lauri Shipitalo, Martin J. Siren, Taisto TI Dew-worms in white nights: High-latitude light constrains earthworm (Lumbricus terrestris) behaviour at the soil surface SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Adaptation; Geographical variation; Illumination; Light environment; Phenotypic flexibility; Sensory ecology ID L.; HETEROGENEITY; OLIGOCHAETA; CARBON; LAND AB Soil is an effective barrier to light penetration that limits the direct influence of light on belowground organisms. Variation in aboveground light conditions, however, is important to soil-dwelling animals that are periodically active on the soil surface. A prime example is the earthworm Lumbricus terrestris L. (the dew-worm), an ecosystem engineer that emerges nocturnally on the soil surface. In the summer, the northernmost populations of L terrestris are exposed to a time interval with no daily dark period. During a two-week period preceding the summer solstice, we studied the constraints that boreal night illumination imposes on L terrestris surface activity by comparing their behaviour under ambient light with artificially-induced darkness. Looking for evidence of geographical divergence in light response, we compared the behaviour of native L terrestris (Joldoinen, S W Finland; 60 degrees 48'N) with two markedly more southern populations, from Preston (Lancashire, UK; 53 degrees 47'N) and Coshocton (Ohio, USA; 40 degrees 22'N) where the nights have a period of darkness throughout the year (total latitudinal range ca. 2300 km). Under ambient light conditions, L terrestris emergence on the soil surface was diminished by half compared with the darkened treatment and it peaked at the darkest period of the night. Also mating rate decreased considerably under ambient light. The native dew-worms were generally the most active under ambient light. They emerged earlier in the evening and ceased their activity later in the morning than dew-worms from the two more southerly populations. The differences in behaviour were, however, significant mainly between native and UK dew-worms. In the darkened treatment, the behaviour of the three earthworm origins did not differ. Under the experimental conditions light condition was the dominant environmental factor controlling surface activity, but elevated night-time air temperature and humidity also encouraged dew-worm emergence without discernible differences among geographical origins. Our results show, that in boreal summer, the high level of night illumination strongly limits soil-surface activity of dew-worms. Considering the important regulatory role of L terrestris in many ecosystem processes, this can have significant corollaries in dew-worm impacts on the environment. Although evidence for geographical differentiation in behaviour was obtained, the results point to phenotypic flexibility in L. terrestris light response. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Nuutinen, Visa; Jauhiainen, Lauri; Siren, Taisto] MTT Agrifood Res Finland, FI-31600 Jokioinen, Finland. [Butt, Kevin R.] Univ Cent Lancashire, Grenfell Baines Sch Architecture Construct & Envi, Preston PR1 2HE, Lancs, England. [Shipitalo, Martin J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Nuutinen, V (reprint author), MTT Agrifood Res Finland, FI-31600 Jokioinen, Finland. EM visa.nuutinen@mtt.fi; KRButt@uclan.ac.uk; Martin.Shipitalo@ARS.USDA.GOV OI Shipitalo, Martin/0000-0003-4775-7345; Butt, Kevin/0000-0003-0886-7795 FU UCLan; MTT FX We thank Jan Valckx, Mika Ramo and Keijo Kuronen for technical advice and assistance, Pauli Rissanen (FMI) for the generous help in the collection of the weather data, David Porco for carrying out the genetic analyses and two anonymous referees for helpful comments. VN and KRB gratefully acknowledge mobility grants from UCLan and MTT. The study was conducted as a part of MTT's VILMA-project. NR 49 TC 1 Z9 2 U1 5 U2 41 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAY PY 2014 VL 72 BP 66 EP 74 DI 10.1016/j.soilbio.2014.01.023 PG 9 WC Soil Science SC Agriculture GA AF8NU UT WOS:000334973700008 ER PT J AU Guthrie, HD Welch, GR Woods, LC AF Guthrie, H. D. Welch, G. R. Woods, L. C., III TI Effects of frozen and liquid hypothermic storage and extender type on calcium homeostasis in relation to viability and ATP content in striped bass (Morone saxatilis) sperm SO THERIOGENOLOGY LA English DT Article DE Flow cytometry; Fluo-3; Calcium homeostasis; Sperm viability ID SHORT-TERM STORAGE; PLASMA-MEMBRANES; BOAR SPERMATOZOA; RAM SPERMATOZOA; MOTILITY; CA2+; CRYOPRESERVATION; ACTIVATION; BULL; OSMOLALITY AB The effect of hypothermic storage on striped bass sperm calcium homeostasis was determined by Fluo-3 flow cytometry. Calcium homeostasis was defined as the ability of cells to maintain a low concentration of intracellular free calcium as measured by Fluo-3 fluorescence. Sperm were stored frozen in striped bass extender (SBE) and Tris-NaCl medium (T350) modified with 50 mM glycine and 7.5% dimethylsulfoxide and in nonfrozen form diluted 1:3 (vol/vol) in SBE and 1350 for 1, 24, and 48 hours at 4 degrees C in an oxygen atmosphere. Fluo-3 fluorescence was detected in less than 5% of fresh viable sperm cells indicating maintenance of calcium homeostasis. In contrast to sperm in fresh semen, frozen-thawed and nonfrozen sperm cells lost to a considerable extent the ability to maintain low intracellular free calcium even in the absence of exogenous calcium; positive Fluo-3 fluorescence was found in 26% and 39% of thawed sperm frozen in SBE- and T350-based freezing diluents, respectively, and increased (P < 0.05) to 67% during nonfrozen storage in SBE and 1350 at 24 and 48 hours. Sperm viability measured by exclusion of propidium iodide by flow cytometry was 99% in fresh milt and maintained at 86% (P> 0.05) in SBE after 48 hours of nonfrozen storage but decreased (P < 0.05) to 55.7% after 48 hours in T350. Energy status in terms of ATP content, determined by luciferin-luciferase bioluminescence assay, was higher (P < 0.05) in sperm frozen in SBE than in T350 during the first 5 minutes post-thaw and decreased to essentially zero by 15 minutes post-thaw and did not differ among nonfrozen storage treatments. In conclusion, sperm cells impervious to propidium iodide after frozen or nonfrozen storage were unable to maintain low intracellular calcium content. SBE is a better medium than T350 for frozen or nonfrozen storage of striped bass sperm. The inability to regulate intracellular calcium in striped bass sperm may be associated with poor activation of motility after 4 degrees C storage and cryopreservation. Published by Elsevier Inc. C1 [Guthrie, H. D.; Welch, G. R.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Woods, L. C., III] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. RP Guthrie, HD (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. EM dave.guthrie@ars.usda.gov NR 38 TC 0 Z9 0 U1 3 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD MAY PY 2014 VL 81 IS 8 BP 1085 EP 1091 DI 10.1016/j.theriogenology.2014.01.035 PG 7 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA AF3YH UT WOS:000334648100010 PM 24576712 ER PT J AU Sandrock, C Tanadini, LG Pettis, JS Biesmeijer, JC Potts, SG Neumann, P AF Sandrock, Christoph Tanadini, Lorenzo G. Pettis, Jeffery S. Biesmeijer, Jacobus C. Potts, Simon G. Neumann, Peter TI Sublethal neonicotinoid insecticide exposure reduces solitary bee reproductive success SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE pollinator; population dynamics; fitness; Osmia; thiamethoxam; pesticide risk assessment; neonicotinoid; sublethal effect; Clothianidin ID RED MASON BEE; HONEY-BEES; OSMIA-RUFA; PESTICIDE EXPOSURE; SEX DETERMINATION; APIS-MELLIFERA; BUMBLE BEES; POLLINATORS; CROPS; HYMENOPTERA AB Pollinating insects provide crucial and economically important ecosystem services to crops and wild plants, but pollinators, particularly bees, are globally declining as a result of various driving factors, including the prevalent use of pesticides for crop protection. Sublethal pesticide exposure negatively impacts numerous pollinator life-history traits, but its influence on reproductive success remains largely unknown. Such information is pivotal, however, to our understanding of the long-term effects on population dynamics. We investigated the influence of field-realistic trace residues of the routinely used neonicotinoid insecticides thiamethoxam and clothianidin in nectar substitutes on the entire life-time fitness performance of the red mason bee Osmia bicornis. We show that chronic, dietary neonicotinoid exposure has severe detrimental effects on solitary bee reproductive output. Neonicotinoids did not affect adult bee mortality; however, monitoring of fully controlled experimental populations revealed that sublethal exposure resulted in almost 50% reduced total offspring production and a significantly male-biased offspring sex ratio. Our data add to the accumulating evidence indicating that sublethal neonicotinoid effects on non-Apis pollinators are expressed most strongly in a rather complex, fitness-related context. Consequently, to fully mitigate long-term impacts on pollinator population dynamics, present pesticide risk assessments need to be expanded to include whole life-cycle fitness estimates, as demonstrated in the present study using O. bicornis as a model. C1 [Sandrock, Christoph] Agroscope Liebefeld Posieux ALP, Swiss Bee Res Ctr, CH-3003 Bern, Switzerland. [Tanadini, Lorenzo G.] Univ Zurich, Balgrist Univ Hosp, Ctr Sci, SCI Res, CH-8008 Zurich, Switzerland. [Pettis, Jeffery S.] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Biesmeijer, Jacobus C.] Netherlands Ctr Biodivers Naturalis, NL-2300 RA Leiden, Netherlands. [Potts, Simon G.] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Neumann, Peter] Univ Bern, Inst Bee Hlth, Vetsuisse Fac, CH-3001 Bern, Switzerland. [Neumann, Peter] Univ Bern, Fac Med, CH-3001 Bern, Switzerland. RP Sandrock, C (reprint author), Agroscope Liebefeld Posieux ALP, Swiss Bee Res Ctr, Schwarzenburgstr 161, CH-3003 Bern, Switzerland. EM ch.sandrock@gmail.com RI Tanadini, Lorenzo/E-7290-2015 OI Tanadini, Lorenzo/0000-0003-4337-0637 FU European Union [244090] FX We are grateful to Matthias Volk at Agroscope Reckenholz-Tanniken, ART, for providing research facilities, technical support and discussions, as well as Robin Dean and Roger Konrad for advice concerning the experimental procedures. We thank Mike Herrmann for helpful suggestions and providing Osmia cocoons, as well as five anonymous reviewers for their constructive comments on the manuscript. This research received funding from the European Union Framework 7 under grant agreement no. 244090 (CP-FP) STEP (Status and Trends in European Pollinators). NR 67 TC 19 Z9 20 U1 31 U2 227 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2014 VL 16 IS 2 BP 119 EP 128 DI 10.1111/afe.12041 PG 10 WC Entomology SC Entomology GA AE5LK UT WOS:000334030300002 ER PT J AU Myers, SW Zylstra, KE Francese, JA Borchert, DM Bailey, SM AF Myers, Scott W. Zylstra, Kelley E. Francese, Joseph A. Borchert, Daniel M. Bailey, Sian M. TI Phenology and flight periodicity of Sirex noctilio (Hymenoptera: Siricidae) in central New York, U.S.A. SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE invasive species; forest pests; European wood wasp; Degree-days ID PINUS; ACCUMULATION; WOODWASP; EGG AB Field and laboratory studies were performed to determine the phenology of flight activity and the thermal requirements for adult emergence of Sirex noctilio. Degree-day (DD) accumulation from egg to adult was measured in bolts of Pinus sylvestris infested with eggs from laboratory-reared adults. Adult emergence was similarly monitored in bolts from trees that were naturally infested in the field by wild populations of S. noctilio reared at constant temperature. Laboratory-infested bolts produced mostly males, whereas field-collected material produced a 2.7 : 1 male-biased sex ratio. Mean DDs to emergence was 1477.0 +/- 13.4 (males) in laboratory-infested bolts, as well as 1455.2 +/- 11.2 (males) and 1577.8 +/- 19.5 (females) in field-collected material. Field-trapping studies were conducted to compare flight activity with rearing data. Trap captures showed first flight activity and peak catch occurred at 709 and 1145 DDs. The resulting degree-day model predicts early flight activity in early to mid-April for pine stands in southeastern U.S.A., early to mid-May in the Mid-Atlantic region, and late June to early July in the northeast. C1 [Myers, Scott W.; Zylstra, Kelley E.; Francese, Joseph A.; Bailey, Sian M.] USDA APHIS, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. [Borchert, Daniel M.] USDA APHIS, Ctr Plant Hlth Sci & Technol, Raleigh, NC 27606 USA. RP Myers, SW (reprint author), USDA APHIS, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. EM scott.w.myers@aphis.usda.gov FU USDA-APHIS Sirex noctilio programme FX The authors wish to thank Mike Crawford and Susan Carlton for their many hours in the field working on the trapping portion of this project. We also thank Miriam Cooperband, Peggy Elder, Colin Geisenhoffer, Helen Hull-Sanders, Patrick Pendergast, Jamie Savje and Rebecca Timson for their contributions and support. Ken Bloem, Robyn Rose and Kimberly Wallin critiqued earlier versions of this manuscript and provided many helpful comments. Funding for this project was provided by the USDA-APHIS Sirex noctilio programme. NR 29 TC 2 Z9 2 U1 0 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2014 VL 16 IS 2 BP 129 EP 135 DI 10.1111/afe.12042 PG 7 WC Entomology SC Entomology GA AE5LK UT WOS:000334030300003 ER PT J AU Pimentel, CS Ayres, MP Vallery, E Young, C Streett, DA AF Pimentel, Carla S. Ayres, Matthew P. Vallery, Erich Young, Christopher Streett, Douglas A. TI Geographical variation in seasonality and life history of pine sawyer beetles Monochamus spp: its relationship with phoresy by the pinewood nematode Bursaphelenchus xylophilus SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE pine forests; zero-inflated negative binomial distribution; plant parasitism; Biological invasions; North America; flight phenology ID COLEOPTERA CERAMBYCIDAE; WILT DISEASE; BODY-SIZE; WOOD NEMATODE; UNITED-STATES; CAROLINENSIS; TRANSMISSION; TITILLATOR; SCUTELLATUS; DISPERSAL AB Bursaphelenchus xylophilus (Steiner & Buhrer) (Nematoda: Aphelenchoididae), the pinewood nematode and the causal agent of the pine wilt disease, is a globally important invasive pathogen of pine forests. It is phoretic in woodborer beetles of the genus Monochamus (Megerle) (Coleoptera, Cerambycidae) and has been able to exploit novel indigenous species of Monochamus (but only Monochamus) in newly-invaded areas. North America (NA) is the continent of origin for the B. xylophilus/Monochamus spp. phoretic system. NA also contains the largest number of Monochamus species known to act as vectors for B. xylophilus. Understanding this phoretic system in its native geographical area helps to explain the evolutionary ecology of pine wilt disease. In the present study, we measured the flight phenology, size, sex ratios and species identity of Monochamus species in five geographically distant forests in NA. We also measured phoresy by B. xylophilus. We found the nematode to be abundant across eastern NA but rare or absent in western NA. In eastern forests, nematode phoresy was highest on the Monochamus species that flew earliest in the year. However, in the southeast, where Monochamus is most likely multivoltine with a long flight season, we found vectors with high nematode loads throughout the season, indicating that B. xylophilus can be transmitted to new hosts during most part of the year. The frequency distribution of nematode dauers on Monochamus was highly aggregated. Bursaphelenchus xylophilus in NA appears to be able to use all available Monochamus species as vectors. In native NA pine forests, the pinewood nematode appears to have an ecology that is sufficiently flexible to exploit different species (and both genders) of Monochamus, and disperse at different times of the year. This flexibility may contribute to its recent success in invading Eurasian pine forests. C1 [Pimentel, Carla S.] Univ Lisbon, Inst Super Agron, Ctr Estudos Florestais, P-1349017 Lisbon, Portugal. [Pimentel, Carla S.; Ayres, Matthew P.] Dartmouth Coll, Dept Biol Sci, Life Sci Ctr 125, Hanover, NH 03755 USA. [Vallery, Erich; Young, Christopher; Streett, Douglas A.] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. RP Pimentel, CS (reprint author), Univ Lisbon, Inst Super Agron, Ctr Estudos Florestais, P-1349017 Lisbon, Portugal. EM carlapimentel@isa.utl.pt RI Pimentel, Carla/P-8252-2014 OI Pimentel, Carla/0000-0002-8364-8990 FU Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-CFL/098869/2008, SFRH/BPD/46995/2008, SFRH/BPD/80867/2011]; Luso-American Development Foundation (FLAD); Dartmouth College; Southern Research Station of the USDA Forest Service FX We are very grateful to Monica Bond for collecting Monochamus in California; to Richard Hofstetter for collecting Monochamusin Arizona; and to Craig Layne, Nina Lany and Jeff Lombardo for helping with insect collections in Vermont and New Jersey. We are also grateful to Robin M. Giblin-Davis and Dale R. Bergdahl for confirming nematode identification, as well as to several anonymous referees for their valuable comments and suggestions. This work was funded by the Portuguese Foundation for Science and Technology (FCT) through the project PTDC/AGR-CFL/098869/2008, and two grants conceded to C. Pimentel: SFRH/BPD/46995/2008 and SFRH/BPD/80867/2011. Additional support was provided by the Luso-American Development Foundation (FLAD) and a cooperative agreement between Dartmouth College and the Southern Research Station of the USDA Forest Service. NR 56 TC 2 Z9 2 U1 1 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2014 VL 16 IS 2 BP 196 EP 206 DI 10.1111/afe.12049 PG 11 WC Entomology SC Entomology GA AE5LK UT WOS:000334030300009 ER PT J AU Busarakam, K Bull, AT Girard, G Labeda, DP van Wezel, GP Goodfellow, M AF Busarakam, Kanungnid Bull, Alan T. Girard, Genevieve Labeda, David P. van Wezel, Gilles P. Goodfellow, Michael TI Streptomyces leeuwenhoekii sp nov., the producer of chaxalactins and chaxamycins, forms a distinct branch in Streptomyces gene trees SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Streptomyces; Polyphasic taxonomy; Atacama Desert; Natural products; Genome sequence ID MULTILOCUS SEQUENCE-ANALYSIS; DNA-DNA HYBRIDIZATION; ATACAMA DESERT SOIL; MAXIMUM-LIKELIHOOD; TAXONOMIC EVALUATION; GENOME SEQUENCE; A-D; SYSTEMATICS; BACTERIAL; CLADE AB A polyphasic study was carried out to establish the taxonomic status of an Atacama Desert isolate, Streptomyces strain C34(T), which synthesises novel antibiotics, the chaxalactins and chaxamycins. The organism was shown to have chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. Analysis of 16S rRNA gene sequences showed that strain C34(T) formed a distinct phyletic line in the Streptomyces gene tree that was very loosely associated with the type strains of several Streptomyces species. Multilocus sequence analysis based on five house-keeping gene alleles underpinned the separation of strain C34(T) from all of its nearest phylogenetic neighbours, apart from Streptomyces chiangmaiensis TA-1(T) and Streptomyces hyderabadensis OU-40(T) which are not currently in the MLSA database. Strain C34(T) was distinguished readily from the S. chiangmaiensis and S. hyderabadensis strains by using a combination of cultural and phenotypic data. Consequently, strain C34(T) is considered to represent a new species of the genus Streptomyces for which the name Streptomyces leeuwenhoekii sp. nov. is proposed. The type strain is C34(T) (= DSM 42122(T) = NRRL B-24963(T)). Analysis of the whole-genome sequence of S. leeuwenhoekii, with 6,780 predicted open reading frames and a total genome size of around 7.86 Mb, revealed a high potential for natural product biosynthesis. C1 [Busarakam, Kanungnid; Goodfellow, Michael] Univ Newcastle, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Bull, Alan T.] Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England. [Girard, Genevieve; van Wezel, Gilles P.] Leiden Univ, Inst Biol, NL-2300 RA Leiden, Netherlands. [Labeda, David P.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Goodfellow, M (reprint author), Univ Newcastle, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. EM m.goodfellow@ncl.ac.uk OI van Wezel, Gilles/0000-0003-0341-1561 FU Thai Royal Government; Netherlands Applied Research Council (STW) [10467]; Royal Society [JP 100654]; Leverhulme Trust FX Kanungnid Busarakam is grateful for a scholarship from the Thai Royal Government, Gilles P. van Wezel for GenExpand Grant No. 10467 from The Netherlands Applied Research Council (STW), Alan T Bull for support from The Royal Society (International Joint Project Grant JP 100654) and Michael Goodfellow for an Emeritus Fellowship from the Leverhulme Trust. The authors are indebted to Dr Byung-Yong Kim for carrying out the fatty acid analysis on the type strain of Streptomyces leeuwenhoekii. NR 67 TC 14 Z9 14 U1 2 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD MAY PY 2014 VL 105 IS 5 BP 849 EP 861 DI 10.1007/s10482-014-0139-y PG 13 WC Microbiology SC Microbiology GA AF0LV UT WOS:000334406700005 PM 24604690 ER PT J AU Kurtzman, CP Robnett, CJ AF Kurtzman, Cletus P. Robnett, Christie J. TI Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov and Middelhovenomyces gen. nov SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Article DE Danielozyma; Deakozyma; Middelhovenomyces; Candida; New yeast genera ID MULTIGENE PHYLOGENETIC ANALYSIS; ASCOMYCETOUS YEASTS; SEQUENCE-ANALYSIS; PROPOSAL; CANDIDA; FUNGI; WOOD AB Three new non-ascosporic, ascomycetous yeast genera are proposed based on their isolation from currently described species and genera. Phylogenetic placement of the genera was determined from analysis of nuclear gene sequences for D1/D2 large subunit rRNA, small subunit rRNA, translation elongation factor-1 alpha and RNA polymerase II, subunits B1 and B2. The new taxa are: Deakozyma gen. nov., type species Deakozyma indianensis sp. nov. (type strain NRRL YB-1937, CBS 12903); Danielozyma gen. nov., type species Danielozyma ontarioensis comb. nov. (type strain NRRL YB-1246, CBS 8502); D. litseae comb. nov. (type strain NRRL YB-3246, CBS 8799); Middelhovenomyces gen. nov., type species Middelhovenomyces tepae comb. nov. (type strain NRRL Y-17670, CBS 5115) and M. petrohuensis comb. nov. (type strain NRRL Y-17663, CBS 8173). C1 [Kurtzman, Cletus P.; Robnett, Christie J.] USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Kurtzman, CP (reprint author), USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov NR 22 TC 7 Z9 7 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 EI 1572-9699 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD MAY PY 2014 VL 105 IS 5 BP 933 EP 942 DI 10.1007/s10482-014-0149-9 PG 10 WC Microbiology SC Microbiology GA AF0LV UT WOS:000334406700014 PM 24633952 ER PT J AU Corella, D Ordovas, JM AF Corella, Dolores Ordovas, Jose M. TI How does the Mediterranean diet promote cardiovascular health? Current progress toward molecular mechanisms SO BIOESSAYS LA English DT Article DE cardiovascular; epigenomics; Mediterranean diet; microRNAs; nutrigenetics; nutrigenomics; stroke ID RANDOMIZED CONTROLLED-TRIAL; CROSS-KINGDOM REGULATION; RISK-FACTORS; FATTY-ACIDS; DNA METHYLATION; METABOLIC SYNDROME; GLYCEMIC LOAD; DISEASE RISK; STYLE DIET; ENDOTHELIAL DYSFUNCTION AB Epidemiological evidence supports a health-promoting effect of the Mediterranean Diet (MedDiet), especially in the prevention of cardiovascular diseases. These cardiovascular benefits have been attributed to a number of components of the MedDiet such as monounsaturated fatty acids, antioxidant vitamins and phytochemicals. However, the underlying mechanisms remain unknown. Likewise, little is known about the genes that define inter-individual variation in response to the MedDiet, although the TCF7L2 gene is emerging as an illustrative candidate for determining relative risk of cardiovascular events in response to the MedDiet. Moreover, omics technologies are providing evidence supporting potential mechanisms, some of them implicating epigenetics (i.e. microRNAs, methylation), and certain data suggest that some traditional foods could contribute via microRNAs possibly acting as exogenous regulators of gene expression. Future research should aim at increasing and consolidating the nutrigenetic and nutrigenomic knowledge of the MedDiet in order to provide sound, personalized and optimized nutritional recommendations. C1 [Corella, Dolores] Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia, Spain. [Corella, Dolores] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid, Spain. [Ordovas, Jose M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Corella, D (reprint author), Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia, Spain. EM dolores.corella@uv.es FU Spanish Ministry of Health (Instituto de Salud Carlos III); Ministry of Economy and Innovation, Spain; Fondo Europeo de Desarrollo Regional [CIBER 06/03, CNIC-06, PI11/02505, AGL2010-22319-C03-03]; Generalitat Valenciana, Spain [ACOMP/2013/165, ACOMP/2013/159]; U.S. Department of Agriculture [58-1950-0-014] FX This study has been supported by The Spanish Ministry of Health (Instituto de Salud Carlos III) and the Ministry of Economy and Innovation, Spain and Fondo Europeo de Desarrollo Regional (projects CIBER 06/03, CNIC-06, PI11/02505, AGL2010-22319-C03-03) and by the Generalitat Valenciana, Spain (ACOMP/2013/165 and ACOMP/2013/159). This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, NR 118 TC 11 Z9 11 U1 0 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0265-9247 EI 1521-1878 J9 BIOESSAYS JI Bioessays PD MAY PY 2014 VL 36 IS 5 BP 526 EP 537 DI 10.1002/bies.201300180 PG 12 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA AE5RR UT WOS:000334047000014 PM 24706458 ER PT J AU Pearson, DE Hierro, JL Chiuffo, M Villarreal, D AF Pearson, D. E. Hierro, J. L. Chiuffo, M. Villarreal, D. TI Rodent seed predation as a biotic filter influencing exotic plant abundance and distribution SO BIOLOGICAL INVASIONS LA English DT Article DE Biotic resistance; Road disturbance; Plant recruitment; Seed predation; Water addition ID APPARENT COMPETITION; PROPAGULE PRESSURE; BIOLOGICAL-CONTROL; INSECT HERBIVORY; OLD-FIELDS; RECRUITMENT; POPULATIONS; INVASIONS; ESTABLISHMENT; COMMUNITIES AB Biotic resistance is commonly invoked to explain why many exotic plants fail to thrive in introduced ranges, but the role of seed predation as an invasion filter is understudied. Abiotic conditions may also influence plant populations and can interact with consumers to determine plant distributions, but how these factors jointly influence invasions is poorly understood. In central Argentina's Caldenal savannas, we experimentally examined how seed predation and water availability influenced recruitment/establishment of nine exotic plant invaders over 2 years. We then explored how seed predation patterns related to invasion patterns. Excluding rodent seed predators dramatically increased seedling recruitment for eight of nine exotic species (by 100-300 % in most cases) and increased young/adult plant abundance for four species in one or both years. Adding water to ameliorate drought tended to increase seedling numbers for most species, but these trends were not significant. Vegetation surveys revealed that exotic plant richness was 50 % lower in matrix habitat compared with disturbed roadsides and that cover of the two most aggressive invaders, which were both strongly suppressed by seed predation, was 75-80 % lower in matrix than roadside habitats. Seed offerings indicated seed removal by rodents was 11 times greater in intact matrix habitat compared with roadsides. Rodent seed predation represents a significant source of biotic resistance to plant invasions. Ubiquitous disturbances such as road construction can disrupt this filter. The widely recognized role that disturbance plays in facilitating invasions, which is largely attributed solely to reduced plant competition, may also arise from disruption of top-down controls. C1 [Pearson, D. E.] USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Pearson, D. E.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Hierro, J. L.; Chiuffo, M.] INCITAP CONICET UNLPam, RA-6300 Santa Rosa, La Pampa, Argentina. [Hierro, J. L.; Villarreal, D.] Univ Nacl La Pampa, Fac Ciencias Exactas & Nat, RA-6300 Santa Rosa, La Pampa, Argentina. RP Pearson, DE (reprint author), USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. EM dpearson@fs.fed.us FU President's Early Career Award in Science and Engineering; CONICET; ANPCyT; UNLPam FX We thank B. Connolly, A. Litt, Y. Ortega, and anonymous reviewers for valuable feedback on draft manuscripts. L. S. Baggett consulted on statistical analyses. We are in debt to R. Lecitra, F. Miguel, N. Icasatti, and M. Cock for field assistance, and personnel at the Parque Luro Provincial Reserve for logistical support. Rainfall information for Santa Rosa was provided by G. Vergara (Agronomy Department, UNLPam). This project was funded by the President's Early Career Award in Science and Engineering to D.E.P. and CONICET, ANPCyT, and UNLPam to J.L.H. NR 60 TC 9 Z9 10 U1 6 U2 60 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD MAY PY 2014 VL 16 IS 5 BP 1185 EP 1196 DI 10.1007/s10530-013-0573-1 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AE7KX UT WOS:000334177900021 ER PT J AU Hesler, LS Losey, JE Allee, LL Smyth, RR AF Hesler, Louis S. Losey, John E. Allee, Leslie L. Smyth, Rebecca Rice TI NEW RECORDS OF COCCINELLIDAE ( COLEOPTERA) FROM WYOMING, USA SO COLEOPTERISTS BULLETIN LA English DT Editorial Material ID SOUTH-DAKOTA; LADY BEETLE; PREDATORS C1 [Hesler, Louis S.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Losey, John E.; Allee, Leslie L.; Smyth, Rebecca Rice] Cornell Univ, Dept Entomol, Lost Ladybug Project, Ithaca, NY 14853 USA. RP Hesler, LS (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov NR 16 TC 0 Z9 0 U1 0 U2 2 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD MAY PY 2014 VL 68 IS 1 BP 128 EP 130 DI 10.1649/0010-065X-68.1.128 PG 3 WC Entomology SC Entomology GA AE2JJ UT WOS:000333798800022 ER PT J AU Hesler, LS AF Hesler, Louis S. TI NEW STATE RECORD FOR CEPHALOSCYMNUS ZIMMERMANNI ZIMMERMANNI CROTCH (COLEOPTERA: COCCINELLIDAE) IN MASSACHUSETTS, USA SO COLEOPTERISTS BULLETIN LA English DT Editorial Material ID AMERICA; NORTH C1 USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Hesler, LS (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov NR 4 TC 0 Z9 0 U1 1 U2 1 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD MAY PY 2014 VL 68 IS 1 BP 156 EP 157 DI 10.1649/0010-065X-68.1.156 PG 2 WC Entomology SC Entomology GA AE2JJ UT WOS:000333798800028 ER PT J AU Vaughn, SF Behle, RW Skory, CD Kurtzman, CP Price, NPJ AF Vaughn, S. F. Behle, R. W. Skory, C. D. Kurtzman, C. P. Price, N. P. J. TI Utilization of sophorolipids as biosurfactants for postemergence herbicides SO CROP PROTECTION LA English DT Article DE Sophorolipids; Biosurfactants; Herbicides; Starmerella; Candida ID NONYLPHENOL ETHOXYLATE; YEAST CLADE; CLOVE OIL; TOXICITY; GLYPHOSATE; FORMULATIONS; SURFACTANTS; AGRICULTURE; PESTICIDES; AMPHIBIANS AB Sophorolipids are carbohydrate-based, amphiphilic biosurfactants produced by several species of the Starmerella yeast clade. Most sophorolipids are partially acetylated sophorose sugars O-beta-glycosidically linked to 17-L-hydroxy-Delta 9-octadecenoic acid, where typically the acyl carboxyl group forms a 4"-lactone to the terminal glucosyl residue. Recently sophorolipids were discovered in which the sophorose is linked to the omega-carbon of the acyl group and occurs predominately in a non-lactone, anionic form. In this study we compared lactone sophorolipids produced by Starmerella (Candida) bombicola (Sb) and non-lactone sophorolipids produced by Candida kuoi (Ck) against a synthetic polyethoxylated tallowamine surfactant (POEA) which is used in commercial postemergence herbicides. When mixed with the lipophilic contact herbicide lemongrass oil (LGO), stable emulsions with Ck lasted longer than with either POEA or Sb. Phytotoxicity (as measured by fresh and dry weights and visual damage three days after spraying) to sicklepod (Senna obtusifolia) by the Ck/LGO and Sb/LGO mixtures were similar to a POEA/LGO mixture, while visual damage to corn (Zea mays L) was increased by the addition of all of the surfactants. When applied together with the water-soluble herbicide phosphinothricin, the Ck/LGO and Sb/LGO treatments caused decreases in sicklepod dry weights and herbicide damage ratings (HDR) compared to phosphinothricin applied without a surfactant ten days after treatment. With corn, POEA and Ck applied with PT had the greatest reductions in fresh and dry weights, and HDR values. These results indicate that sophorolipids have excellent promise as natural surfactants for postemergence herbicides. Published by Elsevier Ltd. C1 [Vaughn, S. F.] USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res, Peoria, IL 61604 USA. [Behle, R. W.] USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res, Peoria, IL 61604 USA. [Skory, C. D.; Price, N. P. J.] USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Product Technol Res, Peoria, IL 61604 USA. [Kurtzman, C. P.] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res, Peoria, IL 61604 USA. RP Vaughn, SF (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Steven.Vaughn@ars.usda.gov NR 38 TC 1 Z9 1 U1 3 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAY PY 2014 VL 59 BP 29 EP 34 DI 10.1016/j.cropro.2014.01.014 PG 6 WC Agronomy SC Agriculture GA AE6VC UT WOS:000334135100005 ER PT J AU Werner, SJ Tupper, SK Pettit, SE Ellis, JW Carlson, JC Goldade, DA Hofmann, NM Homan, HJ Linz, GM AF Werner, Scott J. Tupper, Shelagh K. Pettit, Susan E. Ellis, Jeremy W. Carlson, James C. Goldade, David A. Hofmann, Nicholas M. Homan, H. Jeffrey Linz, George M. TI Application strategies for an anthraquinone-based repellent to protect oilseed sunflower crops from pest blackbirds SO CROP PROTECTION LA English DT Article DE Agelaius phoeniceus; Anthraquinone; Chemical repellent; Esfenvalerate; Helianthus annuus; Pyraclostrobin ID REDUCING BIRD DAMAGE; NEWLY PLANTED RICE; REGISTERED PESTICIDES; LABORATORY EFFICACY; FLIGHT CONTROL(TM); FORMULATION; CAFFEINE; CORN AB Non-lethal alternatives are needed to manage the damage caused by wild birds to oilseed sunflower crops (Helianthus annuus Linnaeus). We evaluated field residues and experimental applications of an anthraquinone-based repellent (active ingredient 50% 9,10-anthraquinone) to minimize red-winged blackbird (Agelaius phoeniceus Linnaeus) depredation of oilseed sunflower. Chemical residues from experimental applications of the anthraquinone-based repellent (4.7 1/ha and 9.4 1/ha; low, high) in a ripening oilseed sunflower field were 481 ppm and 978 ppm anthraquinone at the beginning of blackbird damage, and 385 ppm and 952 ppm anthraquinone at the end of blackbird damage, respectively. Prior to harvest, we observed 402 ppm and 462 ppm anthraquinone in the oil, and 27 ppm and 165 ppm anthraquinone in the pomace from crushed sunflower achenes previously sprayed with the low and high applications, respectively. For the purpose of developing application strategies useful for avian repellents, we subsequently investigated blackbird feeding response to oilseed sunflower treated with the anthraquinone-based repellent and either a registered insecticide or a registered fungicide popularly used for ripening sunflower. We observed a positive concentration response relationship among blackbirds exposed to anthraquinone and the insecticide (a.i. 8.4% esfenvalerate), or anthraquinone and the fungicide (a.i. 23.6% pyraclostrobin). Blackbirds reliably discriminated between untreated sunflower and that treated with 1810 ppm anthraquinone and 0.1% of the insecticide or 1700 ppm anthraquinone and 0.14% of the fungicide during our preference experiments. Given that ripening achenes are inverted from conventional pesticide applications throughout much of the period associated with blackbird depredation, we also evaluated blackbird repellency of the anthraquinone-based repellent applied to involucral bracts (i.e., the back of sunflower heads) of oilseed sunflower. Blackbirds did not discriminate between untreated involucral bracts and those treated with foliar applications comparable to 4.7 1/ha or 9.4 1/ha; blackbirds consumed more achenes from untreated sunflower heads than from those treated with 18.7 1/ha of the anthraquinone-based repellent. Supplemental repellent efficacy studies should investigate blackbird response to anthraquinone-based repellents (e.g., >= 4.7 1/ha) within 10-100 ha sunflower fields and include independent field replicates with predicted bird damage, repellent application strategies developed for protection of ripening crops, pre- and at-harvest repellent residues, and bird damage and crop yield measurements. Published by Elsevier Ltd. C1 [Werner, Scott J.; Tupper, Shelagh K.; Pettit, Susan E.; Ellis, Jeremy W.; Carlson, James C.; Goldade, David A.; Hofmann, Nicholas M.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Ft Collins, CO 80521 USA. [Homan, H. Jeffrey; Linz, George M.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv,North Dakota Field Stn, Bismarck, ND 58501 USA. RP Werner, SJ (reprint author), USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Scott.J.Werner@aphis.usda.gov OI Ellis, Jeremy/0000-0003-4512-0906 FU National Sunflower Association (Mandan, ND, USA) FX This research was supported by the National Sunflower Association (Mandan, ND, USA). Our feeding experiments were conducted with the anthraquinone-based repellent (Arkion (R) Life Sciences, New Castle, DE, USA), Asana (R) XL insecticide (DuPont, Wilmington, DE, USA) and Headline (R) fungicide (BASF Corp., Research Triangle Park, NC, USA). Corporate collaborations do not imply endorsement by the United States Department of Agriculture. We thank W. Bleier (North Dakota State University [NDSU], Fargo, ND, USA), T. DeKrey, M. Klosterman, B. Larson, L. Penry, J. Schanandore, M. Strassburg and D. Stonefish for their dedicated assistance with our 2010 sunflower field study. D. Weiskopf (USDA Agricultural Research Service EARS], Fargo, ND, USA) shelled sunflower head samples and D.P. Wiesenborn (NDSU, Fargo) provided assistance and equipment for preparation of oil and pomace samples from our field study. M.F. Vigil and D.G. Koch (USDA ARS, Arkron, CO, USA) provided assistance and equipment for stationary threshing of achene samples and M.M. Santer (Arkion (R) Life Sciences) conducted analytical chemistry for our field study. We appreciate the National Wildlife Research Center animal care staff that provided daily care of all birds throughout quarantine and holding for our repellent efficacy studies. We also thank L. Addy Orduna, S.B. Canavelli, J.D. Eisemann, B.A. Kimball, A.M. Mangan, E.N. Rodriguez and M.E. Tobin for constructive feedback from their review of our manuscript. NR 27 TC 6 Z9 6 U1 1 U2 18 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAY PY 2014 VL 59 BP 63 EP 70 DI 10.1016/j.cropro.2014.01.016 PG 8 WC Agronomy SC Agriculture GA AE6VC UT WOS:000334135100010 ER PT J AU Showler, AT Moran, PJ AF Showler, Allan T. Moran, Patrick J. TI Associations between host plant concentrations of selected biochemical nutrients and Mexican rice borer, Eoreuma loftini, infestation SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE free amino acids; Poaceae; sorghum; Lepidoptera; histidine; fructose; sugarcane; Crambidae; sudangrass; sugars; preference; stalkborer; johnsongrass; maize ID EUROPEAN CORN-BORER; LEPIDOPTERA CRAMBIDAE; STEM-BORERS; OVIPOSITION; SUGARCANE; RESPONSES; SORGHUM; TEXAS AB The Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae), is an economic pest of sugarcane (Saccharum spp. hybrids) and other graminaceous host crops, and it attacks grassy weeds. Oviposition preference has been known to be for plants with leaves that form folds. This study is the first to associate the nutritional quality of crop and forage plant hosts with Mexican rice borer injury. Three experiments were conducted to determine the levels of selected biochemical nutrients, including free amino acids and sugars, in four grass weeds [barnyardgrass, Echinochloa crus-galli (L.); broadleaf signalgrass, Urochloa platyphylla (Munro ex C. Wright); johnsongrass, Sorghum halepense (L.) Pers.; and Vasey's grass, Paspalum urvillei Steud.], a forage grass [sudangrass, Sorghum bicolor (L.) Moensch spp. drummondi (Nees ex Steud.) de Wet & Harlan], three crop species [maize, Zea mays L.; sorghum, Sorghum bicolor (L.) Moench; and sugarcane, Saccharum spp.], and a sorghum-sudangrass forage hybrid (all Poaceae). Of 16 free amino acids detected among plants in the first two experiments, only high accumulations of free histidine in sudangrass and maize were associated with increased infestations by Mexican rice borer larvae. In all three experiments, high levels of fructose were associated with heightened Mexican rice borer infestation. Ramifications of these findings on the potential dispersal of this invasive pest in the USA and possible applications of fructose in baits are discussed. C1 [Showler, Allan T.] ARS, USDA, Kerrville, TX 78028 USA. [Moran, Patrick J.] ARS, USDA, Albany, CA 94710 USA. RP Showler, AT (reprint author), ARS, USDA, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM allan.showler@ars.usda NR 38 TC 6 Z9 6 U1 3 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAY PY 2014 VL 151 IS 2 BP 135 EP 143 DI 10.1111/eea.12177 PG 9 WC Entomology SC Entomology GA AE8ZA UT WOS:000334290400006 ER PT J AU Harvey, BC Railsback, SF AF Harvey, Bret C. Railsback, Steven F. TI Feeding modes in stream salmonid population models: is drift feeding the whole story? SO ENVIRONMENTAL BIOLOGY OF FISHES LA English DT Article DE Drift; Search feeding; Foraging mode; Individual-based modeling; Salmonidae ID JUVENILE COHO SALMON; GRAYLING THYMALLUS-ARCTICUS; INDIVIDUAL-BASED MODEL; NET ENERGY-INTAKE; RAINBOW-TROUT; DOMINANCE HIERARCHIES; INVERTEBRATE DRIFT; MOUNTAIN STREAMS; INTERIOR ALASKA; POSITION CHOICE AB Drift-feeding models are essential components of broader models that link stream habitat to salmonid populations and community dynamics. But is an additional feeding mode needed for understanding and predicting salmonid population responses to streamflow and other environmental factors? We addressed this question by applying two versions of the individual-based model inSTREAM to a field experiment in which streamflow was varied in experimental units that each contained a stream pool and the adjacent upstream riffle. The two model versions differed only in the feeding options available to fish. Both versions of inSTREAM included drift feeding; one also included a search feeding mode to represent feeding in which food availability is largely independent of streamflow, such as feeding from the benthos, or feeding from the water column or the water's surface in low water velocities. We compared the abilities of the two model versions to fit the observed distributions of growth by individual rainbow trout (Oncorhynchus mykiss) in the field experiment. The version giving fish the daily choice between drift or search feeding better fit observations than the version in which fish fed only on drift. Values for drift and search food availability from calibration to the individual mass changes of fish in experimental units with unaltered streamflow yielded realistic distributions of individual growth when applied to experimental units in which streamflow was reduced by 80 %. These results correspond with empirical studies that show search feeding can be an important alternative to drift feeding for salmonids in some settings, and indicate that relatively simple formulations of both processes in individual-based population models can be useful in predicting the effects of environmental alterations on fish populations. C1 [Harvey, Bret C.] US Forest Serv, Pacific Southwest Res Stn, Arcata, CA 95521 USA. [Railsback, Steven F.] Humboldt State Univ, Dept Math, Arcata, CA 95521 USA. RP Harvey, BC (reprint author), US Forest Serv, Pacific Southwest Res Stn, Arcata, CA 95521 USA. EM bharvey@fs.fed.us OI Railsback, Steven/0000-0002-5923-9847 NR 31 TC 9 Z9 9 U1 2 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0378-1909 EI 1573-5133 J9 ENVIRON BIOL FISH JI Environ. Biol. Fishes PD MAY PY 2014 VL 97 IS 5 SI SI BP 615 EP 625 DI 10.1007/s10641-013-0186-7 PG 11 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA AE6SU UT WOS:000334126100015 ER PT J AU Davies, KW Bates, JD Madsen, MD Nafus, AM AF Davies, K. W. Bates, J. D. Madsen, M. D. Nafus, A. M. TI Restoration of Mountain Big Sagebrush Steppe Following Prescribed Burning to Control Western Juniper SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Aerial seeding; Artemisia tridentata; Fire; Habitat; Recovery; Sage-grouse ID SAGE-GROUSE; PLANT-COMMUNITIES; CRESTED WHEATGRASS; NORTH-AMERICA; CENTRAL UTAH; VEGETATION; FIRE; CONSERVATION; POPULATIONS; DISPERSAL AB Western juniper (Juniperus occidentalis ssp. occidentalis Hook) encroachment into mountain big sagebrush (Artemisia tridentata spp. vaseyana (Rydb.) Beetle) steppe has reduced livestock forage production, increased erosion risk, and degraded sagebrush-associated wildlife habitat. Western juniper has been successfully controlled with partial cutting followed by prescribed burning the next fall, but the herbaceous understory and sagebrush may be slow to recover. We evaluated the effectiveness of seeding perennial herbaceous vegetation and sagebrush at five sites where juniper was controlled by partially cutting and prescribed burning. Treatments tested at each site included an unseeded control, herbaceous seed mix (aerially seeded), and the herbaceous seed mix plus sagebrush seed. In the third year post-treatment, perennial grass cover and density were twice as high in plots receiving the herbaceous seed mix compared to the control plots. Sagebrush cover and density in the sagebrush seeded plots were between 74- and 290-fold and 62- and 155-fold greater than the other treatments. By the third year after treatment, sagebrush cover was as high as 12 % in the sagebrush seeded plots and between 0 % and 0.4 % where it was not seeded. These results indicate that aerial seeding perennial herbaceous vegetation can accelerate the recovery of perennial grasses which likely stabilize the site. Our results also suggest that seeding mountain big sagebrush after prescribed burning encroaching juniper can rapidly recover sagebrush cover and density. In areas where sagebrush habitat is limited, seeding sagebrush after juniper control may increase sagebrush habitat and decrease the risks to sagebrush-associated species. C1 [Davies, K. W.; Bates, J. D.; Madsen, M. D.] USDA ARS, Burns, OR 97720 USA. [Nafus, A. M.] Oregon State Univ, Burns, OR USA. RP Davies, KW (reprint author), USDA ARS, 67826-A Hwy 205, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu FU Burns District-Bureau of Land Management; USDA-Agricultural Research Service FX The authors thank the Burns District-Bureau of Land Management for allowing this research to be conducted on lands they administer and for providing some of the funds used to carry out this study. Additional funding for this research project was provided by the USDA-Agricultural Research Service. The authors also thank Julie Garner, Sarah Fitzpatrick, Brandi Carlon, Rodney Johnson, Savannah Duff, Alanna Riggs, Tim Rowe, and Anne Crecelius for assisting with data collection. We also are grateful to Dustin Johnson and Brenda Smith for reviewing earlier versions of this manuscript. We appreciate the thoughtful reviews by the anonymous reviewers and Editorial Board Member. NR 39 TC 13 Z9 13 U1 2 U2 42 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD MAY PY 2014 VL 53 IS 5 BP 1015 EP 1022 DI 10.1007/s00267-014-0255-5 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AE7KM UT WOS:000334176700011 PM 24573166 ER PT J AU Lee, S Jia, M Jia, YL Liu, GJ AF Lee, Seonghee Jia, Melisa H. Jia, Yulin Liu, Guangjie TI Tagging quantitative trait loci for heading date and plant height in important breeding parents of rice (Oryza sativa) SO EUPHYTICA LA English DT Article DE Heading date; Marker-assisted selection; Plant height; Quantitative trait loci; Rice (Oryza sativa) ID ADVANCED BACKCROSS POPULATION; EPISTATIC INTERACTIONS; GREEN-REVOLUTION; YIELD COMPONENTS; AGRONOMIC TRAITS; FLOWERING TIME; PHOTOPERIOD SENSITIVITY; TESTCROSS POPULATIONS; ALPHA-SUBUNIT; INBRED LINES AB Heading date and plant height are important determinants for plant growth rate. In this study, simple sequence repeat markers were used to tag quantitative trait loci (QTL) using a recombinant inbred line mapping population derived from two important breeding parents, genetic stock Kaybonnetlpa1-1 and indica cultivar Zhe733, using data collected under field and greenhouse conditions. Interval mapping, composite interval mapping, and multiple interval mapping were performed to map QTL for heading date and plant height, and to identify epistatic interactions between the QTL. qHD3.1 on chromosome 3 from KBNTlpa1-1 had the largest effect on heading date contributing an average of 28.4 % of the total phenotypic variation. qHD7.1, 7.2, and 8.1 also had a significant contribution to heading date from Zhe733 averaging 8.1, 12.8, and 12.8 % of the phenotypic variance, respectively, and there was a positive additive-by-additive epistatic interaction between qHD7.1 and qHD8.1. QTL, qPHT1.1 and qPHT3.1, for plant height were detected on chromosomes 1 and 3, respectively. qPHT1.1 contributed the largest effect representing 38.2 % of the total phenotypic variation. Comparison of the QTL identified in our study with previous results revealed that the chromosomal locations for QTL coincided closely with positions reported previously in other rice populations worldwide, suggesting that these QTL have coevolved and become domesticated. The tightly linked SSR markers that flank these QTL should be desirable for tagging heading date and plant height genes and facilitating their incorporation into advanced breeding lines using marker assisted selection. C1 [Lee, Seonghee; Liu, Guangjie] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Jia, Melisa H.; Jia, Yulin] USDA ARS, Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Jia, YL (reprint author), USDA ARS, Natl Rice Res Ctr, Dale Bumpers, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov FU Arkansas Rice Research and Promotion Board FX The authors thank the Arkansas Rice Research and Promotion Board for financial support; Michael Lin, Tracy Bianco, Ellen McWhirter, Alan Sites, Tony Beaty, and Dr. Joseph Kepiro for excellent technical support; and Lorie Bernhardt and Dr. J. Neil Rutger of Genetic Stock-Oryza (GSOR) collection of Dale Bumpers National Rice Research Center for providing the RIL population of the cross of KBNTlpa1-1 and Zhe733. USDA is an equal opportunity provider and employer. NR 50 TC 5 Z9 6 U1 3 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2014 VL 197 IS 2 BP 191 EP 200 DI 10.1007/s10681-013-1051-7 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AF0ZP UT WOS:000334444200003 ER PT J AU Yu, XM Price, NPJ Evans, BS Metcalf, WW AF Yu, Xiaomin Price, Neil P. J. Evans, Bradley S. Metcalf, William W. TI Purification and Characterization of Phosphonoglycans from Glycomyces sp Strain NRRL B-16210 and Stackebrandtia nassauensis NRRL B-16338 SO JOURNAL OF BACTERIOLOGY LA English DT Article ID 2-AMINOETHYLPHOSPHONIC ACID; BACTEROIDES-FRAGILIS; NMR-SPECTROSCOPY; PLASMA-MEMBRANE; POLY(GLYCEROPHOSPHATE) GLYCEROPHOSPHOTRANSFERASE; CAPSULAR POLYSACCHARIDES; ACANTHAMOEBA-CASTELLANII; TEICHOIC-ACIDS; CDP-GLYCEROL; BIOSYNTHESIS AB Two related actinomycetes, Glycomyces sp. strain NRRL B-16210 and Stackebrandtia nassauensis NRRL B-16338, were identified as potential phosphonic acid producers by screening for the gene encoding phosphoenolpyruvate (PEP) mutase, which is required for the biosynthesis of most phosphonates. Using a variety of analytical techniques, both strains were subsequently shown to produce phosphonate-containing exopolysaccharides (EPS), also known as phosphonoglycans. The phosphonoglycans were purified by sequential organic solvent extractions, methanol precipitation, and ultrafiltration. The EPS from the Glycomyces strain has a mass of 40 to 50 kDa and is composed of galactose, xylose, and five distinct partially O-methylated galactose residues. Per-deutero-methylation analysis indicated that galactosyl residues in the polysaccharide backbone are 3,4-linked Gal, 2,4-linked 3-MeGal, 2,3-linked Gal, 3,6-linked 2MeGal, and 4,6-linked 2,3-diMeGal. The EPS from the Stackebrandtia strain is comprised of glucose, galactose, xylose, and four partially O-methylated galactose residues. Isotopic labeling indicated that the O-methyl groups in the Stackebrandtia phosphonoglycan arise from S-adenosylmethionine. The phosphonate moiety in both phosphonoglycans was shown to be 2-hydroxyethylphosphonate (2-HEP) by P-31 nuclear magnetic resonance (NMR) and mass spectrometry following strong acid hydrolysis of the purified molecules. Partial acid hydrolysis of the purified EPS from Glycomyces yielded 2-HEP in ester linkage to the O-5 or O-6 position of a hexose and a 2-HEP mono(2,3-dihydroxypropyl) ester. Partial acid hydrolysis of Stackebrandtia EPS also revealed the presence of 2-HEP mono(2,3-dihydroxypropyl) ester. Examination of the genome sequences of the two strains revealed similar pepM-containing gene clusters that are likely to be required for phosphonoglycan synthesis. C1 [Yu, Xiaomin; Metcalf, William W.] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA. [Yu, Xiaomin; Evans, Bradley S.; Metcalf, William W.] Univ Illinois, Inst Genom Biol, Urbana, IL USA. [Price, Neil P. J.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Metcalf, WW (reprint author), Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA. EM metcalf@illinois.edu RI Evans, Bradley/M-2842-2013; OI Evans, Bradley/0000-0002-1207-9006 FU National Institutes of Health [PO1 GM077596]; NIH [S10 RR028833] FX This work was supported by the National Institutes of Health (PO1 GM077596). NMR spectra were recorded on a 600-MHz instrument purchased with support from NIH grant S10 RR028833. NR 42 TC 6 Z9 6 U1 4 U2 15 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0021-9193 EI 1098-5530 J9 J BACTERIOL JI J. Bacteriol. PD MAY PY 2014 VL 196 IS 9 BP 1768 EP 1779 DI 10.1128/JB.00036-14 PG 12 WC Microbiology SC Microbiology GA AF0NM UT WOS:000334411300014 PM 24584498 ER PT J AU Al-Madhhachi, AT Fox, GA Hanson, GJ Tyagi, AK Bulut, R AF Al-Madhhachi, A. T. Fox, G. A. Hanson, G. J. Tyagi, A. K. Bulut, R. TI Mechanistic Detachment Rate Model to Predict Soil Erodibility Due to Fluvial and Seepage Forces SO JOURNAL OF HYDRAULIC ENGINEERING LA English DT Article DE Seepage; Detachment rate model; Cohesive soils; Flume; Jet erosion test ID GROUND-WATER SEEPAGE; EROSION; JET AB The erosion rate of cohesive soils is typically computed using an excess shear stress model based on the applied fluvial shear stress. However, no mechanistic approaches are available for incorporating additional forces, such as groundwater seepage into the excess shear stress model parameters. Seepage forces are known to be significant contributors to streambank erosion and failure. The objective of this research was to incorporate seepage forces into a mechanistic fundamental detachment rate model to improve the predictions of the erosion rate of cohesive soils. The new detachment model, which is referred to as the modified Wilson model, was based on two modified dimensional soil parameters (b0 and b1) that included seepage forces due to localized groundwater flow. The proposed model provided a general framework for studying the impact of soil properties, fluid characteristics, and seepage forces on cohesive soil erodibility. Equations were presented for deriving the material parameters from both flume experiments and jet erosion tests (JETs). In order to investigate the influence of seepage on erodibility, the erodibility of two cohesive soils (silty sand and clayey sand) was measured in flume tests and with a new miniature version of the JET device (mini JET). The soils were packed in three equal lifts in a standard mold (for JETs) and in a soil box (for flume tests) at a uniform bulk density (1.5 and 1.6 Mg/m3) near the soil's optimum water content. A seepage column was utilized to induce a constant hydraulic gradient on the soils tested in the flume and with the mini JET. The modified Wilson model parameters, b0 and b1, were derived from the erosion rate data both with and without the influence of seepage from the flume and JETs. Seepage forces had a significant but nonuniform influence on the derived b0 and b1 as functions of the hydraulic gradient and soil density. The more fundamental detachment model can be used in place of the excess shear stress model with parameters that can be derived using JETs, transforming the modeling of cohesive soils for hillslopes, embankments, gullies, streambeds, and streambanks. C1 [Al-Madhhachi, A. T.] Al Mustansiriya Univ, Dept Environm Engn, Coll Engn, Baghdad 10047, Iraq. [Fox, G. A.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. [Hanson, G. J.] ARS, USDA, Hydraul Lab, Stillwater, OK 74075 USA. [Tyagi, A. K.] Oklahoma State Univ, Sch Civil & Environm Engn, Oklahoma Infrastruct Consortium, Stillwater, OK 74078 USA. [Bulut, R.] Oklahoma State Univ, Sch Civil & Environm Engn, Stillwater, OK 74078 USA. RP Fox, GA (reprint author), Oklahoma State Univ, Dept Biosyst & Agr Engn, 120 Ag Hall, Stillwater, OK 74078 USA. EM abdu@okstate.edu; garey.fox@okstate.edu; greg.hanson@ars.usda.gov; tyagi@okstate.edu; rifat.bulut@okstate.edu FU National Science Foundation [0943491] FX This research is based upon work supported by the National Science Foundation under Grant No. 0943491. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The authors acknowledge Mohammad Rahi and David Criswell, Oklahoma State University, for assisting with the flume experiments. NR 25 TC 4 Z9 4 U1 3 U2 23 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 EI 1943-7900 J9 J HYDRAUL ENG JI J. Hydraul. Eng.-ASCE PD MAY 1 PY 2014 VL 140 IS 5 DI 10.1061/(ASCE)HY.1943-7900.0000836 PG 12 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA AE8NJ UT WOS:000334258300011 ER PT J AU Kannan, N Jeong, J Arnold, J Gosselink, L Glick, R Srinivasan, R AF Kannan, Narayanan Jeong, Jaehak Arnold, Jeffrey Gosselink, Leila Glick, Roger Srinivasan, Raghavan TI Hydrologic Modeling of a Retention Irrigation System SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Watershed; Best management practice; Flooding; Irrigation; Algorithm; Urban; Storm water; Modeling; Retention AB Urbanized watersheds produce instantaneous response to rainfall, resulting in storm-water runoff exceeding the carrying capacity of drainage systems-the management of which is important to prevent flooding and erosion of streams. Management can be achieved using structural storm-water best management practices (BMPs). The city of Austin, Texas, envisioned a plan to mitigate future scenarios for flooding and erosion, resulting in the development and integration of storm-water BMP algorithms into the subhourly version of the soil and water assessment tool (SWAT) model. Algorithms were developed to simulate the physical processes governing the flow transport mechanisms through these BMPs. They were tested using a previously flow-calibrated watershed in the Austin, Texas, area. The retention irrigation (RI) system is one such BMP commonly found in Austin, Texas. This paper describes the development of a modeling tool for the RI system and its potential applications. From the test results obtained, it appears that the RI algorithm functions rationally. The algorithm developed could be used to evaluate the functionality of an individual BMP structure to analyze the benefits of such structures at a watershed scale and as a design tool. C1 [Kannan, Narayanan] Tarleton State Univ, Texas Inst Appl Environm Res, Stephenville, TX 76401 USA. [Kannan, Narayanan] Tetra Tech, Lafayette, CA 94549 USA. [Jeong, Jaehak] Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Arnold, Jeffrey] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Gosselink, Leila; Glick, Roger] City Austin, Watershed Protect Dept, Austin, TX 78704 USA. [Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, College Stn, TX 77843 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. RP Kannan, N (reprint author), Tarleton State Univ, Texas Inst Appl Environm Res, Stephenville, TX 76401 USA. EM kannan@tiaer.tarleton.edu; jjeong@brc.tamus.edu; jeff.arnold@ars.usda.gov; leila.gosselink@austintexas.gov; roger.glick@austintexas.gov; r-srinivasan@tamu.edu RI Srinivasan, R/D-3937-2009 NR 6 TC 0 Z9 0 U1 2 U2 24 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD MAY 1 PY 2014 VL 19 IS 5 BP 1036 EP 1041 DI 10.1061/(ASCE)HE.1943-5584.0000867 PG 6 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA AE9SA UT WOS:000334348100017 ER PT J AU Lewis, NS Anderson, TK Kitikoon, P Skepner, E Burke, DF Vincent, AL AF Lewis, Nicola S. Anderson, Tavis K. Kitikoon, Pravina Skepner, Eugene Burke, David F. Vincent, Amy L. TI Substitutions near the Hemagglutinin Receptor-Binding Site Determine the Antigenic Evolution of Influenza A H3N2 Viruses in US Swine SO JOURNAL OF VIROLOGY LA English DT Article ID UNITED-STATES; GENETIC EVOLUTION; PIGS; SUBTYPE; HUMANS; TRANSMISSION; IMMUNIZATION; POPULATION; PROTECTION; INFECTION AB Swine influenza A virus is an endemic and economically important pathogen in pigs, with the potential to infect other host species. The hemagglutinin (HA) protein is the primary target of protective immune responses and the major component in swine influenza A vaccines. However, as a result of antigenic drift, vaccine strains must be regularly updated to reflect currently circulating strains. Characterizing the cross-reactivity between strains in pigs and seasonal influenza virus strains in humans is also important in assessing the relative risk of interspecies transmission of viruses from one host population to the other. Hemagglutination inhibition (HI) assay data for swine and human H3N2 viruses were used with antigenic cartography to quantify the antigenic differences among H3N2 viruses isolated from pigs in the United States from 1998 to 2013 and the relative cross-reactivity between these viruses and current human seasonal influenza A virus strains. Two primary antigenic clusters were found circulating in the pig population, but with enough diversity within and between the clusters to suggest updates in vaccine strains are needed. We identified single amino acid substitutions that are likely responsible for antigenic differences between the two primary antigenic clusters and between each antigenic cluster and outliers. The antigenic distance between current seasonal influenza virus H3 strains in humans and those endemic in swine suggests that population immunity may not prevent the introduction of human viruses into pigs, and possibly vice versa, reinforcing the need to monitor and prepare for potential incursions. C1 [Lewis, Nicola S.; Skepner, Eugene; Burke, David F.] Univ Cambridge, Dept Zool, Cambridge, England. [Anderson, Tavis K.; Kitikoon, Pravina; Vincent, Amy L.] USDA ARS, Virus & Prion Res Unit, Ames, IA 50011 USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Prion Res Unit, Ames, IA 50011 USA. EM amy.vincent@ars.usda.gov OI burke, david/0000-0001-8830-3951; Anderson, Tavis/0000-0002-3138-5535 FU USDA-ARS; USDA-APHIS; USDA-ARS SCA [58-3625-2-103F]; EC FP7 [259949] FX Funding was provided by USDA-ARS and USDA-APHIS. N.S.L. was funded by USDA-ARS SCA agreement number 58-3625-2-103F and EC FP7 award number 259949. NR 54 TC 15 Z9 15 U1 1 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD MAY PY 2014 VL 88 IS 9 BP 4752 EP 4763 DI 10.1128/JVI.03805-13 PG 12 WC Virology SC Virology GA AE9TT UT WOS:000334353900014 PM 24522915 ER PT J AU Palti, Y Gao, GT Miller, MR Vallejo, RL Wheeler, PA Quillet, E Yao, JB Thorgaard, GH Salem, M Rexroad, CE AF Palti, Yniv Gao, Guangtu Miller, Michael R. Vallejo, Roger L. Wheeler, Paul A. Quillet, Edwige Yao, Jianbo Thorgaard, Gary H. Salem, Mohamed Rexroad, Caird E., III TI A resource of single-nucleotide polymorphisms for rainbow trout generated by restriction-site associated DNA sequencing of doubled haploids SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE rainbow trout; doubled haploid; paralogous sequence variants; single-nucleotide polymorphism discovery; restriction-site-associated DNA sequencing ID WESTSLOPE CUTTHROAT TROUT; ONCORHYNCHUS-MYKISS; SNP DISCOVERY; FISH GENOMES; RAD MARKERS; SYNTENY; TRAITS; SUSCEPTIBILITY; DUPLICATION; ADAPTATION AB Salmonid genomes are considered to be in a pseudo-tetraploid state as a result of a genome duplication event that occurred between 25 and 100Ma. This situation complicates single-nucleotide polymorphism (SNP) discovery in rainbow trout as many putative SNPs are actually paralogous sequence variants (PSVs) and not simple allelic variants. To differentiate PSVs from simple allelic variants, we used 19 homozygous doubled haploid (DH) lines that represent a wide geographical range of rainbow trout populations. In the first phase of the study, we analysed SbfI restriction-site associated DNA (RAD) sequence data from all the 19 lines and selected 11 lines for an extended SNP discovery. In the second phase, we conducted the extended SNP discovery using PstI RAD sequence data from the selected 11 lines. The complete data set is composed of 145168 high-quality putative SNPs that were genotyped in at least nine of the 11 lines, of which 71446 (49%) had minor allele frequencies (MAF) of at least 18% (i.e. at least two of the 11 lines). Approximately 14% of the RAD SNPs in this data set are from expressed or coding rainbow trout sequences. Our comparison of the current data set with previous SNP discovery data sets revealed that 99% of our SNPs are novel. In the support files for this resource, we provide annotation to the positions of the SNPs in the working draft of the rainbow trout reference genome, provide the genotypes of each sample in the discovery panel and identify SNPs that are likely to be in coding sequences. C1 [Palti, Yniv; Gao, Guangtu; Vallejo, Roger L.; Rexroad, Caird E., III] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. [Miller, Michael R.] Univ Oregon, Inst Mol Biol, Eugene, OR 97403 USA. [Miller, Michael R.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Wheeler, Paul A.; Thorgaard, Gary H.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Wheeler, Paul A.; Thorgaard, Gary H.] Washington State Univ, Ctr Reprod Biol, Pullman, WA 99164 USA. [Quillet, Edwige] INRA, GABI, UMR 1313, F-78350 Jouy En Josas, France. [Yao, Jianbo; Salem, Mohamed] W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. [Salem, Mohamed] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. RP Palti, Y (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM yniv.palti@ars.usda.gov RI Gao, Guangtu/F-4541-2012 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67015-30091] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30091 from the USDA National Institute of Food and Agriculture. We thank Roseanna Long and Kristy Shewbridge for their technical assistance and Dr. Alvaro Hernandez, biotechnology core facility of the University of Illinois, for suggestions on how to improve the RAD libraries sequencing efficiency. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 29 TC 14 Z9 14 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAY PY 2014 VL 14 IS 3 BP 588 EP 596 DI 10.1111/1755-0998.12204 PG 9 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA AE8HH UT WOS:000334240000015 PM 24251403 ER PT J AU Siris, E Adler, R Bilezikian, J Bolognese, M Dawson-Hughes, B Favus, M Harris, S Jan de Beur, S Khosla, S Lane, N Lindsay, R Nana, A Orwoll, E Saag, K Silverman, S Watts, N AF Siris, E. S. Adler, R. Bilezikian, J. Bolognese, M. Dawson-Hughes, B. Favus, M. J. Harris, S. T. Jan de Beur, S. M. Khosla, S. Lane, N. E. Lindsay, R. Nana, A. D. Orwoll, E. S. Saag, K. Silverman, S. Watts, N. B. TI The clinical diagnosis of osteoporosis: a position statement from the National Bone Health Alliance Working Group SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE Clinical diagnosis; Criteria; Osteoporosis; Position statement ID MINERAL DENSITY; POSTMENOPAUSAL WOMEN; ZOLEDRONIC ACID; UNITED-STATES; HIP FRACTURE; RISK; THRESHOLDS; THERAPY AB Osteoporosis causes an elevated fracture risk. We propose the continued use of T-scores as one means for diagnosis but recommend that, alternatively, hip fracture; osteopenia-associated vertebral, proximal humerus, pelvis, or some wrist fractures; or FRAX scores with a parts per thousand yen3 % (hip) or 20 % (major) 10-year fracture risk also confer an osteoporosis diagnosis. Osteoporosis is a common disorder of reduced bone strength that predisposes to an increased risk for fractures in older individuals. In the USA, the standard criterion for the diagnosis of osteoporosis in postmenopausal women and older men is a T-score of a parts per thousand currency signaEuro parts per thousand a'2.5 at the lumbar spine, femur neck, or total hip by bone mineral density testing. Under the direction of the National Bone Health Alliance, 17 clinicians and clinical scientists were appointed to a working group charged to determine the appropriate expansion of the criteria by which osteoporosis can be diagnosed. The group recommends that postmenopausal women and men aged 50 years should be diagnosed with osteoporosis if they have a demonstrable elevated risk for future fractures. This includes having a T-score of less than or equal to -2.5 at the spine or hip as one method for diagnosis but also permits a diagnosis for individuals in this population who have experienced a hip fracture with or without bone mineral density (BMD) testing and for those who have osteopenia by BMD who sustain a vertebral, proximal humeral, pelvic, or, in some cases, distal forearm fracture. Finally, the term osteoporosis should be used to diagnose individuals with an elevated fracture risk based on the World Health Organization Fracture Risk Algorithm, FRAX. As new ICD-10 codes become available, it is our hope that this new understanding of what osteoporosis represents will allow for an appropriate diagnosis when older individuals are recognized as being at an elevated risk for fracture. C1 [Siris, E. S.; Bilezikian, J.] Columbia Univ, Med Ctr, Dept Med, Div Endocrinol, New York, NY 10027 USA. [Adler, R.] Virginia Commonwealth Univ, Sch Med, Richmond, VA USA. [Bolognese, M.] Bethesda Hlth Res, Bethesda, MD USA. [Dawson-Hughes, B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Bone Metab Lab, Boston, MA USA. [Favus, M. J.] Univ Chicago, Dept Med, Chicago, IL 60637 USA. [Harris, S. T.] Univ Calif San Francisco, Dept Endocrinol & Metab, San Francisco, CA 94143 USA. [Jan de Beur, S. M.] Johns Hopkins Univ, Div Endocrinol, Baltimore, MD USA. [Khosla, S.] Mayo Clin, Rochester, MN USA. [Lane, N. E.] Univ Calif Davis, Sch Med, Davis, CA 95616 USA. [Lindsay, R.] Helen Hayes Hosp, W Haverstraw, NY USA. [Nana, A. D.] Univ N Texas, Hlth Sci Ctr, Ft Worth, TX USA. [Orwoll, E. S.] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Saag, K.] Univ Alabama Birmingham, Div Clin Immunol & Rheumatol, Birmingham, AL 35294 USA. [Silverman, S.] Cedars Sinai Med Ctr, Bone Hlth Ctr, Los Angeles, CA 90048 USA. [Watts, N. B.] Mercy Hlth Phys, Osteoporosis & Bone Hlth Serv, Cincinnati, OH USA. RP Siris, E (reprint author), Columbia Univ, Med Ctr, Dept Med, Div Endocrinol, New York, NY 10027 USA. EM es27@cumc.columbia.edu OI Khosla, Sundeep/0000-0002-2936-4372 FU Amgen; NPS; NIH; Agnovos; Servier; Merck; Eli Lilly FX ESS is a consultant for Amgen, Eli Lilly, Merck, Novartis, and Pfizer. RA has no conflicts to disclose. JB is a consultant for Amgen, Lilly, Merck, NPS, Bristol Meyers Squibb, and Johnson & Johnson and has research grants from Amgen, NPS, and NIH. MB has no conflicts to disclose. BDH is on the scientific advisory board of Eli Lilly and Agnovos, is a consultant for Agnovos and Servier, and has research grants from Agnovos and Servier. MJF has no conflicts to disclose. STH is a consultant for Amgen, Eli Lilly, Gilead Sciences, and Merck. SMJ has no conflicts to disclose. SK is on the scientific advisory board of Amgen and Bone Therapeutics. NEL has no conflicts to disclose. RL has no conflicts to disclose. ADN has no conflicts to disclose. ESO is on the scientific advisory board of Merck, Eli Lilly, and Wright Medical Tech and has research grants from Amgen, Merck, and Eli Lilly. KS has no conflicts to disclose. SS has no conflicts to disclose. NBW has no conflicts to disclose. NR 22 TC 48 Z9 50 U1 1 U2 13 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD MAY PY 2014 VL 25 IS 5 BP 1439 EP 1443 DI 10.1007/s00198-014-2655-z PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AF0OU UT WOS:000334414700002 PM 24577348 ER PT J AU Belz, RG Duke, SO AF Belz, Regina G. Duke, Stephen O. TI Herbicides and plant hormesis SO PEST MANAGEMENT SCIENCE LA English DT Review DE hormesis; herbicide; growth stimulation; glyphosate ID DOSE-RESPONSE; GROWTH-STIMULATION; GLYPHOSATE; INHIBITORS; TOXICOLOGY; SUGARCANE; RIPENERS; MIXTURES; INCREASE; DEPENDS AB Herbicide hormesis is commonly observed at subtoxic doses of herbicides and other phytotoxins. The occurrence and magnitude of this phenomenon are influenced by plant growth stage and physiological status, environmental factors, the endpoint measured and the timing between treatment and endpoint measurement. The mechanism in some cases of herbicide hormesis appears to be related to the target site of the herbicide, whereas in other examples hormesis may be by overcompensation to moderate stress induced by the herbicides or a response to disturbed homeostasis. Theoretically, herbicide hormesis could be used in crop production, but this has been practical only in the case of the use of herbicides as sugar cane 'ripeners' to enhance sucrose accumulation. The many factors that can influence the occurrence, the magnitude and the dose range of hormetic increases in yield for most crops make it too unpredictable and risky as a production practice with the currently available knowledge. Herbicide hormesis can cause undesired effects in situations in which weeds are unintentionally exposed to hormetic doses (e.g. in adjacent fields, when shielded by crop vegetation). Some weeds that have evolved herbicide resistance may have hormetic responses to recommended herbicide application rates. Little is known about such effects under field conditions. A more complete understanding of herbicide hormesis is needed to exploit its potential benefits and to minimize its potential harmful effects in crop production. (c) 2014 Society of Chemical Industry C1 [Belz, Regina G.] Univ Hohenheim, Agroecol Unit, D-70593 Stuttgart, Germany. [Duke, Stephen O.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS USA. RP Belz, RG (reprint author), Univ Hohenheim, Agroecol Unit 380B, D-70593 Stuttgart, Germany. EM regina.belz@uni-hohenheim.de FU German Research Foundation (DFG) [BE 4189/1-1] FX RG Belz was funded by the German Research Foundation (DFG individual grant, project BE 4189/1-1). NR 59 TC 17 Z9 19 U1 9 U2 85 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2014 VL 70 IS 5 BP 698 EP 707 DI 10.1002/ps.3726 PG 10 WC Agronomy; Entomology SC Agriculture; Entomology GA AE8XB UT WOS:000334285200003 PM 24446388 ER PT J AU Siegfried, BD Rangasamy, M Wang, HC Spencer, T Haridas, CV Tenhumberg, B Sumerford, DV Storer, NP AF Siegfried, Blair D. Rangasamy, Murugesan Wang, Haichuan Spencer, Terence Haridas, Chirakkal V. Tenhumberg, Brigitte Sumerford, Douglas V. Storer, Nicholas P. TI Estimating the frequency of Cry1F resistance in field populations of the European corn borer (Lepidoptera: Crambidae) SO PEST MANAGEMENT SCIENCE LA English DT Article DE pair mating; Bt resistance; allele frequencies; F1 and F2 screening; European corn borer ID BACILLUS-THURINGIENSIS TOXIN; HELICOVERPA-ARMIGERA LEPIDOPTERA; OSTRINIA-NUBILALIS LEPIDOPTERA; ALLELES CONFERRING RESISTANCE; INSECT RESISTANCE; BT CROPS; CROSS-RESISTANCE; TRANSGENIC CROPS; FITNESS COSTS; F-2 SCREEN AB BACKGROUND Transgenic corn hybrids that express toxins from Bacillus thuringiensis (Bt) have suppressed European corn borer populations and reduced the pest status of this insect throughout much of the US corn belt. A major assumption of the high-dose/refuge strategy proposed for insect resistance management and Bt corn is that the frequency of resistance alleles is low so that resistant pests surviving exposure to Bt corn will be rare. RESULTS The frequency of resistance to the Cry1F Bt toxin was estimated using two different screening tools and compared with annual susceptibility monitoring based on diagnostic bioassays and LC50 and EC50 determinations. An F-1 screening approach where field-collected individuals were mated to a resistant laboratory strain and progeny were assayed to determine genotype revealed that resistance alleles could be recovered even during the first year of commercially available Cry1F corn (2003). Estimates of frequency from 2003-2005 and 2006-2008 indicated that, although allele frequency was higher than theoretical assumptions (0.0286 and 0.0253 respectively), there was no indication that the frequency was increasing. Similar estimates in 2008 and 2009 using an F-2 screening approach confirmed the presence of non-rare resistance alleles (frequency approximate to 0.0093 and 0.0142 for 2008 and 2009, respectively). The results of both screening methods were in general agreement with the observed mortality in diagnostic bioassays and LC50 and EC50 determinations. CONCLUSIONS These results are consistent with previous modeling results, suggesting that the high-dose/refuge strategy that is in place for Bt corn may be effective in delaying resistance evolution even when a relatively high frequency of resistance alleles exists. (c) 2013 Society of Chemical Industry C1 [Siegfried, Blair D.; Rangasamy, Murugesan; Wang, Haichuan; Spencer, Terence] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Haridas, Chirakkal V.; Tenhumberg, Brigitte] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68583 USA. [Sumerford, Douglas V.] Iowa State Univ, USDA ARS, Corn Insect & Crop Genet Res Unit, Ames, IA USA. [Storer, Nicholas P.] Dow AgroSci, Indianapolis, IN USA. RP Siegfried, BD (reprint author), Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. EM bsiegfried1@unl.edu FU Dow AgroSciences; USDA NIFA Biotechnology Risk Assessment Grant Program [2006-3352-17394, 2010-33522-21673] FX The authors gratefully acknowledge the support of Dow AgroSciences and the USDA NIFA Biotechnology Risk Assessment Grant Program (numbers 2006-3352-17394 and 2010-33522-21673). The Agricultural Biotechnology Stewardship Technical Committee provided support for annual bioassays. The thoughtful and critical review of this paper by BE Tabashnik is also gratefully acknowledged. NR 53 TC 12 Z9 12 U1 0 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2014 VL 70 IS 5 BP 725 EP 733 DI 10.1002/ps.3662 PG 9 WC Agronomy; Entomology SC Agriculture; Entomology GA AE8XB UT WOS:000334285200006 PM 24124030 ER PT J AU Fettig, CJ Munson, AS Grosman, DM Bush, PB AF Fettig, Christopher J. Munson, A. Steven Grosman, Donald M. Bush, Parshall B. TI Evaluations of emamectin benzoate and propiconazole for protecting individual Pinus contorta from mortality attributed to colonization by Dendroctonus ponderosae and associated fungi SO PEST MANAGEMENT SCIENCE LA English DT Article DE lodgepole pine; insecticides; fungicides; mountain pine beetle; tree injections; Scolytinae ID BARK BEETLE ATTACK; LODGEPOLE PINE; COLEOPTERA; CURCULIONIDAE; TEMPERATURE; SCOLYTIDAE; CARBARYL; TREES; INSECTICIDES; PERMETHRIN AB BACKGROUND Protection of conifers from bark beetle colonization typically involves applications of liquid formulations of contact insecticides to the tree bole. An evaluation was made of the efficacy of bole injections of emamectin benzoate alone and combined with the fungicide propiconazole for protecting individual lodgepole pine, Pinus contorta Dougl. ex Loud., from mortality attributed to colonization by mountain pine beetle, Dendroctonus ponderosae Hopkins, and progression of associated blue stain fungi. RESULTS Injections of emamectin benzoate applied in mid-June did not provide adequate levels of tree protection; however, injections of emamectin benzoate + propiconazole applied at the same time were effective for two field seasons. Injections of emamectin benzoate and emamectin benzoate + propiconazole in mid-September provided tree protection the following field season, but unfortunately efficacy could not be determined during a second field season owing to insufficient levels of tree mortality observed in the untreated control, indicative of low D. ponderosae populations. CONCLUSION Previous evaluations of emamectin benzoate for protecting P. contorta from mortality attributed to D. ponderosae have failed to demonstrate efficacy, which was later attributed to inadequate distribution of emamectin benzoate following injections applied several weeks before D. ponderosae colonization. The present data indicate that injections of emamectin benzoate applied in late summer or early fall will provide adequate levels of tree protection the following summer, and that, when emamectin benzoate is combined with propiconazole, tree protection is afforded the year that injections are implemented. Published 2013. This article is a U.S. Government work and is in the public domain in the USA. C1 [Fettig, Christopher J.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Munson, A. Steven] US Forest Serv, USDA, Ogden, UT USA. [Grosman, Donald M.] Arborjet Inc, Woburn, MA USA. [Bush, Parshall B.] Univ Georgia, Agr & Environm Serv Labs, Athens, GA 30602 USA. RP Fettig, CJ (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, 1731 Res Pk Dr, Davis, CA 95618 USA. EM cfettig@fs.fed.us FU USDA Forest Service Pesticide Impact Assessment Program [FS-PIAP R1/4-2009-02] FX The authors thank D Blackford, V DeBlander, L Dunning, C Hayes, L Hebertson and J Neumann (Forest Health Protection, USDA Forest Service), C Dabney and S McKelvey (formerly Pacific Southwest Research Station, USDA Forest Service), J Runyon (Rocky Mountain Research Station, USDA Forest Service), W Upton (Texas A&M Forest Service), and N Bond (The University of Georgia) for technical assistance. In addition, they thank J Gibson and J Schramm of the Uinta-Wasatch-Cache National Forest (Utah) for providing access to the study site, and J Doccola (Arborjet Inc.) for loaning equipment and donating insecticides. This research was supported, in part, by a USDA Forest Service Pesticide Impact Assessment Program grant (FS-PIAP R1/4-2009-02). This publication reports research involving pesticides. It does not contain recommendations for their use, nor does it imply that the uses discussed here have been registered. All uses of pesticides in the United States must be registered by appropriate State and/or Federal agencies before they can be recommended. This article was written and prepared by US Government employees on official time, and it is therefore in the public domain and not subject to copyright. NR 38 TC 4 Z9 4 U1 3 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2014 VL 70 IS 5 BP 771 EP 778 DI 10.1002/ps.3612 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA AE8XB UT WOS:000334285200012 PM 23868360 ER PT J AU Garber, NP Cotty, PJ AF Garber, N. P. Cotty, P. J. TI Aspergillus parasiticus Communities Associated with Sugarcane in the Rio Grande Valley of Texas: Implications of Global Transport and Host Association Within Aspergillus Section Flavi SO PHYTOPATHOLOGY LA English DT Article ID VEGETATIVE COMPATIBILITY GROUPS; AFLATOXIN CONTAMINATION; UNITED-STATES; SOUTH TEXAS; CYCLOPIAZONIC ACID; GENETIC DIVERSITY; CROP-ROTATION; COMMERCIAL COTTONSEED; ATOXIGENIC STRAIN; SOIL POPULATIONS AB In the Rio Grande Valley of Texas (RGV), values of maize and cottonseed crops are significantly reduced by aflatoxin contamination. Aflatoxin contamination of susceptible crops is the product of communities of aflatoxin producers and the average aflatoxin-producing potentials of these communities influence aflatoxin contamination risk. Cropping pattern influences community composition and, thereby, the epidemiology of aflatoxin contamination. In 2004, Aspergillus parasiticus was isolated from two fields previously cropped to sugarcane but not from 23 fields without recent history of sugarcane cultivation. In 2004 and 2005, A. parasiticus composed 18 to 36% of Aspergillus section Flavi resident in agricultural soils within sugarcane-producing counties. A. parasiticus was not detected in counties that do not produce sugarcane. Aspergillus section Flavi soil communities within sugarcane-producing counties differed significantly dependent on sugarcane cropping history. Fields cropped to sugarcane within the previous 5 years had greater quantities of A. parasiticus (mean = 16 CFU/g) than fields not cropped to sugarcane (mean = 0.1 CFU/g). The percentage of Aspergillus section Flavi composed of A. parasiticus increased to 65% under continuous sugarcane cultivation and remained high the first season of rotation out of sugarcane. Section Flavi communities in fields rotated to non-sugarcane crops for 3 to 5 years were composed of <5% A. parasiticus, and fields with no sugarcane history averaged only 0.2% A. parasiticus. The section Flavi community infecting RGV sugarcane stems ranged from 95% A. parasiticus in billets prepared for commercial planting to 52% A. parasiticus in hand-collected sugarcane stems. Vegetative compatibility assays and multilocus phylogenies verified that aflatoxin contamination of raw sugar was previously attributed to similar A. parasiticus in Japan. Association of closely related A. parasiticus genotypes with sugarcane produced in Japan and RGV, frequent infection of billets by these genotypes, and the ephemeral nature of A. parasiticus in RGV soils suggests global transport with sugarcane planting material. C1 [Garber, N. P.; Cotty, P. J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Cotty, P. J.] Univ Arizona, USDA ARS, Tucson, AZ USA. RP Cotty, PJ (reprint author), Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu FU Agricultural Research Service, United States Department of Agriculture, CRIS project [5347-42000-020-00D, 58-6435-9-398, 6435-42000-022-06S] FX This research was supported by the Agricultural Research Service, United States Department of Agriculture, CRIS project 5347-42000-020-00D, and Standard Cooperative Agreement 58-6435-9-398 Project 6435-42000-022-06S. We thank S. Gonzales at Rio Grande Valley Sugar Growers for his informative and accurate maps of RGV sugarcane fields, S. Sparks at SRS Farms for access to his fields and crops, and N. Glynn for useful discussions on sugarcane varietal breeding and testing. NR 94 TC 1 Z9 1 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 BP 462 EP 471 DI 10.1094/PHYTO-04-13-0108-R PG 10 WC Plant Sciences SC Plant Sciences GA AF1ZS UT WOS:000334513000004 PM 24224872 ER PT J AU Jin, F Bai, GH Zhang, DD Dong, YH Ma, LJ Bockus, W Dowell, F AF Jin, Feng Bai, Guihua Zhang, Dadong Dong, Yanhong Ma, Lingjian Bockus, William Dowell, Floyd TI Fusarium-Damaged Kernels and Deoxynivalenol in Fusarium-Infected US Winter Wheat SO PHYTOPATHOLOGY LA English DT Article ID HEAD BLIGHT RESISTANCE; QUANTITATIVE TRAIT LOCUS; SPRING WHEAT; FIELD CONDITIONS; ACCUMULATION; QTLS; INOCULATION; POPULATIONS; CULTIVARS; CULMORUM AB Fusarium head blight (FHB) is a devastating disease that threatens wheat (Triticum aestivum) production in many areas worldwide. FHB infection results in Fusarium-damaged kernels (FDK) and deoxynivalenol (DON) that dramatically reduce grain yield and quality. More effective and accurate disease evaluation methods are imperative for successful identification of FHB-resistant sources and selection of resistant cultivars. To determine the relationships among different types of resistance, 363 (74 soft and 289 hard) U.S. winter wheat accessions were repeatedly evaluated for FDK and DON concentration in greenhouse and field experiments. Single-kernel near-infrared (SKNIR)-estimated FDK and DON were compared with visually estimated FDK and gas chromatography-mass spectroscopy-estimated DON. Significant correlations were detected between percentage of symptomatic spikelets and visual FDK in the greenhouse and field, although correlations were slightly lower in the field. High correlation coefficients also were observed between visually scored FDK and SKNIR-estimated FDK (0.72, P < 0.001) and SKNIR-estimated DON (0.68, P < 0.001); therefore, both visual scoring and SKNIR methods are useful for estimating FDK and DON in breeding programs. C1 [Jin, Feng; Ma, Lingjian] Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China. [Jin, Feng; Bai, Guihua; Zhang, Dadong] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Bockus, William] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Dowell, Floyd] USDA ARS, Engn & Wind Eros Res Unit, Manhattan, KS 66502 USA. RP Bai, GH (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. EM guihua.bai@ars.usda.gov; malingjian@nwsuaf.edu.cn FU U.S. Wheat and Barley Scab Initiative; National Research Initiative Competitive Grants CAP project from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This project was partly funded by the U.S. Wheat and Barley Scab Initiative and the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. The United States Department of Agriculture (USDA) is an equal opportunity provider and employer. Mention of trade names or NR 26 TC 3 Z9 3 U1 0 U2 26 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 BP 472 EP 478 DI 10.1094/PHYTO-07-13-0187-R PG 7 WC Plant Sciences SC Plant Sciences GA AF1ZS UT WOS:000334513000005 PM 24400658 ER PT J AU Ma, JB Jaraba, J Kirkpatrick, TL Rothrock, CS AF Ma, Jianbing Jaraba, Juan Kirkpatrick, Terrence L. Rothrock, Craig S. TI Effects of Meloidogyne incognita and Thielaviopsis basicola on Cotton Growth and Root Morphology SO PHYTOPATHOLOGY LA English DT Article ID SYSTEM MORPHOLOGY; ALFALFA SEEDLINGS; POPULATIONS; INFECTION; PYTHIUM; SOILS; FIELD AB Effects of the root-knot nematode Meloidogyne incognita and the fungal pathogen Thielaviopsis basicola on cotton seedling growth and root morphology were evaluated in controlled environmental experiments. Four pathogen treatments, including noninfested soil, soil infested with M. incognita, soil infested with T. basicola, and soil infested with both pathogens were evaluated at soil bulk densities (BDs) of 1.25 and 1.50 g/cm3. Plant growth and the morphology of the root systems were evaluated 44 days after planting. Infestation with M. incognita and T. basicola together significantly reduced seedling emergence, number of stem nodes, and root system volume compared with either pathogen alone. Either M. incognita or T. basicola reduced plant height, root fresh weight, top dry weight; root parameters total root length, surface area, and links; and root topological parameters magnitude, altitude, and exterior path length. M. incognita infection increased root radius. Root colonization by T. basicola increased with the presence of M. incognita at the lower soil BD. In contrast to previous research with Pythium spp., root topological indices (TIs) were similar with all of the treatments. Root TIs were near 1.92, indicating a herringbone (less branching) root architectural structure. Studying root architecture using a topological model offers an additional approach to evaluating fungi and nematodes and their interactions for soilborne-pathogen systems. C1 [Ma, Jianbing] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Jaraba, Juan] Univ Cordoba, Monteria, Cordoba, Colombia. [Kirkpatrick, Terrence L.] Southwest Res & Extens Ctr, Hope, AR 71801 USA. [Rothrock, Craig S.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. RP Kirkpatrick, TL (reprint author), Southwest Res & Extens Ctr, Hope, AR 71801 USA. EM tkirkpatrick@uaex.edu FU Arkansas Cotton State Support Committee; Cotton, Inc. [08-326AR] FX This research was supported, in part, by the Arkansas Cotton State Support Committee and Cotton, Inc. (08-326AR). NR 29 TC 0 Z9 0 U1 5 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2014 VL 104 IS 5 BP 507 EP 512 DI 10.1094/PHYTO-06-12-0120-R PG 6 WC Plant Sciences SC Plant Sciences GA AF1ZS UT WOS:000334513000009 PM 24283540 ER PT J AU Kou, LP Luo, YG Park, E Turner, ER Barczak, A Jurick, WM AF Kou, Liping Luo, Yaguang Park, Eunhee Turner, Ellen R. Barczak, Anna Jurick, Wayne M., II TI Temperature abuse timing affects the rate of quality deterioration of commercially packaged ready-to-eat baby spinach. Part I: Sensory analysis and selected quality attributes SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Baby spinach; Ready-to-eat; Temperature abuse; Sensory attributes; Quality ID SHELF-LIFE; CONTROLLED-ATMOSPHERE; STORAGE-TEMPERATURE; ICEBERG LETTUCE; TIME; LEAVES; VEGETABLES; PRODUCE; GROWTH; AIR AB Temperature abuse of fresh-cut products occurs routinely during transport and retail store display. However, the stage of product shelf life during temperature abuse and its impact on sensory attributes have not been studied. This study evaluated the effect of temperature abuse occurring immediately after processing and late in shelf life through measurements of sensory attributes, and membrane integrity of commercially packaged ready-to-eat baby spinach. The packaged products were received within 2 days of processing. Samples subject to early temperature abuse were immediately placed at 1, 4, 8, 12, 16 and 20 C storage upon arrival, and those subject to late temperature abuse were stored at 1 degrees C for six days, and then transferred to 4, 8, 12, 16 and 20 degrees C storage. Package headspace gas composition, in-package visual appeal, purchase intent, product color, off-odor, decay, texture, overall quality, and tissue electrolyte leakage were evaluated every 1-2 day up to 16 day total. Results indicate that when the product temperature is maintained at 1-4 degrees C, the quality of commercially packaged baby spinach can be retained for up to 18 days post-processing. However, storage temperature of 8 C or above, significantly (P<0.001) shortened product shelf life as exhibited by accelerated tissue electrolyte leakage, product yellowing, decay and off-odor development. Most importantly, the product's shelf life stage significantly affected its response to temperature. Quality deterioration proceeded more rapidly when temperature abuse occurred in late as opposed to early shelf life stage. Published by Elsevier B.V. C1 [Kou, Liping; Luo, Yaguang; Park, Eunhee; Turner, Ellen R.; Jurick, Wayne M., II] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. [Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling, Shaanxi, Peoples R China. [Luo, Yaguang; Barczak, Anna] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Luo, YG (reprint author), USDA ARS, Food Qual Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov FU USDA-NIFA (Specialty Crop Research Initiative) [2010-01165]; USDA-ARS [1275-43440-004-00D] FX The authors wish to thank Dr. Roger Bilingsley and Ms. Belinda Jones at Dole Fresh Vegetables for donating packaged baby spinach products. This research is supported by USDA-NIFA (Specialty Crop Research Initiative Award no. 2010-01165) USDA-ARS (Project no. 1275-43440-004-00D). NR 28 TC 8 Z9 8 U1 3 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAY PY 2014 VL 91 BP 96 EP 103 DI 10.1016/j.postharvbio.2013.12.025 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AE6IT UT WOS:000334094800013 ER PT J AU Smilanick, JL Mansour, M Sorenson, D AF Smilanick, Joseph L. Mansour, Monir Sorenson, David TI Performance of fogged disinfectants to inactivate conidia of Penicillium digitatum within citrus degreening rooms SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Green mold; Glutaraldehyde; Hydrogen peroxide; Acetic acid; Peracetic acid ID HYDROGEN-PEROXIDE; PERACETIC-ACID; LEMON PACKINGHOUSES; POSTHARVEST CONTROL; MONILINIA-LAXA; DECAY CONTROL; FRUIT; FUMIGATION; STRATEGIES; EQUIPMENT AB Fogging with formaldehyde of citrus packinghouses when the fruit are absent is a practice to control conidia of Penicillium digitatum (Pers.) Sacc., the cause of citrus green mold. Replacements for formaldehyde in these facilities are needed because of worker and environmental health issues. To evaluate the effectiveness of candidate sanitizers, craft wood sticks with conidia of P. digitatum were attached throughout commercial citrus ethylene degreening rooms and either water alone or the sanitizers were applied. The rooms were 20 +/- 2 degrees C and humidified to 85-90% relative humidity. Aldehydes, peroxygen compounds, sodium hypochlorite, chlorine dioxide, quaternary ammonium, alcohols, one phenolic compound, and one organic acid were applied with a compressed air assisted atomizer or fan atomizer in a volume of approximately 6 L per 100 m(3) of room volume dispensed over a 3 h period. Rates applied were expressed as active ingredient per m3 of room volume. All were compared to formaldehyde applied at 1.98 gm(-3) of room volume. After 24 h, the craft wood sticks were retrieved, and germination of the conidia assessed. Five sanitizers reduced germination of conidia by more than 95% and equaled formaldehyde in effectiveness. They were (effective rates): (1) glutaraldehyde (0.1 g m(-3)); (2) hydrogen peroxide (4.4 g m(-3)); (3) Citrisol (1.0 gm(-3)), a proprietary mineral oxychloride oxidizer; (4) acetic acid (5.3 gm(-3)); and (5) peracetic acid (2.4 gm(-3)). The toxicity of effective sanitizers was determined by exposure of P. digitatum conidia for 10 min to concentrations of each and the proportion of survivors used to estimate EC50 and EC99 concentrations. The toxicity of the sanitizers in this assay did not predict their effectiveness when applied by fogging, probably because other factors, such as distribution, persistence, droplet size, or vapor pressure also influenced their effectiveness. Published by Elsevier B.V. C1 [Smilanick, Joseph L.; Mansour, Monir] USDA ARS, San Joaquin Agr Sci Ctr, Parlier, CA 93648 USA. [Sorenson, David] Fruit Growers Supply, Exeter, CA 93221 USA. RP Smilanick, JL (reprint author), 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM joe.smilanick@ars.usda.gov FU California Citrus Research Board CRB [5600 106] FX This project was made possible by a grant from the California Citrus Research Board CRB (Project 5600 106). We acknowledge the technical assistance of Bruce Adams, Julie Doctor, and Marty Coertz of Fruit Growers Supply Packing Services of Exeter California, Gabriel Verduzco, Monir Mansour, and Dennis Margosan of the USDA ARS, Robert Guerra for the design and fabrication of fogging equipment, and Ed Russell and Chang-lin Xiao for critical review of the manuscript. We thank Lynn Loken, Richard Varley Joe Fitz-george, Scott Lawhon, Tarcisio Ruiz, and Art Dawson for product donations, and Gene Taylor, Jeff Sheets, Terry Orr, Jim Williams, and Joe Martinez of the Exeter Citrus Association, Brock Bovetti and Tony Flores of the Sierra Citrus Association, Bob Johnson and Kevin Severns of the Orange Cove Sanger Citrus Association, John Kalendar and John Clower of the Visalia Citrus Packing Group, Don Dames of the Ventura Pacific Montalvo facility. NR 40 TC 2 Z9 2 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAY PY 2014 VL 91 BP 134 EP 140 DI 10.1016/j.postharvbio.2013.12.020 PG 7 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AE6IT UT WOS:000334094800018 ER PT J AU Spielmann, G Johnston, CA O'Connor, DP Foreyt, JP Simpson, RJ AF Spielmann, G. Johnston, C. A. O'Connor, D. P. Foreyt, J. P. Simpson, R. J. TI Excess body mass is associated with T cell differentiation indicative of immune ageing in children SO CLINICAL AND EXPERIMENTAL IMMUNOLOGY LA English DT Article DE overweight; obesity; herpes viruses; immunosenescence; adolescents ID QUALITY-OF-LIFE; METABOLICALLY HEALTHY; CARDIOVASCULAR RISK; TELOMERE LENGTH; OBESITY; INFLAMMATION; ADOLESCENTS; SUBSETS; MEMORY; CYTOMEGALOVIRUS AB Obesity has been associated with accelerated biological ageing and immunosenescence. As the prevalence of childhood obesity is increasing, we wanted to determine if associations between obesity and immunosenescence would manifest in children. We studied 123 Mexican American adolescents aged 10-14 (mean 12 center dot 3 +/- 0 center dot 7) years, with body weights ranging from 30 center dot 1 to 115 center dot 2 kg (mean 52 center dot 5 +/- 14 center dot 5 kg). Blood samples were obtained to determine proportions of naive, central memory (CM), effector memory (EM), senescent and early, intermediate and highly differentiated subsets of CD4(+) and CD8(+) T cells. Overweight and obese children had significantly lowered proportions of early CD8(+) T cells (B = -11 center dot 55 and -5 center dot 51%, respectively) compared to healthy weight. Overweight children also had more EM (B = +7 center dot 53%), late (B = +8 center dot 90%) and senescent (B = +4 center dot 86%) CD8(+) T cells than healthy weight children, while obese children had more intermediate CD8(+) (B = +4 center dot 59%), EM CD8(+) (B = +5 center dot 49%), late CD4(+) (B = +2 center dot 01%) and senescent CD4(+) (B = +0 center dot 98%) T cells compared to healthy weight children. These findings withstood adjustment for potentially confounding variables, including age, gender and latent cytomegalovirus and Epstein-Barr virus infections. We conclude that excess body mass, even in adolescence, may accelerate immunosenescence and predispose children to increased risks of incurring immune-related health problems in adulthood. C1 [Spielmann, G.; O'Connor, D. P.; Simpson, R. J.] Univ Houston, Lab Integrated Physiol, Dept Hlth & Human Performance, Houston, TX 77204 USA. [Spielmann, G.; O'Connor, D. P.; Simpson, R. J.] Univ Houston, Texas Obes Res Ctr, Houston, TX 77204 USA. [Johnston, C. A.; Foreyt, J. P.] Baylor Coll Med, Dept Pediat Nutr, USDA, ARS,Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Spielmann, G (reprint author), Univ Houston, Lab Integrated Physiol, Dept Hlth & Human Performance, 3855 Holman St, Houston, TX 77204 USA. EM gspielma@central.uh.edu NR 45 TC 7 Z9 7 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0009-9104 EI 1365-2249 J9 CLIN EXP IMMUNOL JI Clin. Exp. Immunol. PD MAY PY 2014 VL 176 IS 2 BP 246 EP 254 DI 10.1111/cei.12267 PG 9 WC Immunology SC Immunology GA AE5LN UT WOS:000334030600012 PM 24401077 ER PT J AU Mourtzinis, S Arriaga, FJ Bransby, D Balkcom, KS AF Mourtzinis, Spyridon Arriaga, Francisco J. Bransby, David Balkcom, Kipling S. TI A simplified method for monomeric carbohydrate analysis of corn stover biomass SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE corn biomass; National Renewable Energy Laboratory; neutral detergent fiber; simplified method; theoretical ethanol yield; carbohydrates AB Constituent determination of biomass for theoretical ethanol yield (TEY) estimation requires the removal of nonstructural carbohydrates prior to analysis to prevent interference with the analytical procedure. According to the accepted US Department of Energy-National Renewable Energy Laboratory (NREL) method, biomass extractives in corn stover should be removed by a two-step extraction process. Alternatively, Neutral Detergent Fiber method (NDF) is a fast and cost-effective method used to determine structural carbohydrates of forage biomass. The objective of this study was to determine whether the NDF method can be used as an alternative to the NREL extraction procedure for corn (Zea mays L.) stover biomass. Arabinan content varied between the two methods. However, glucan, xylan, and TEYs were not statistically different. Furthermore, the average TEYs per unit of mass and unit of area varied by 2.0 and 1.9%, respectively. Additionally, the NDF extraction procedure was faster and less expensive than the NREL procedure. C1 [Mourtzinis, Spyridon; Bransby, David] Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. [Arriaga, Francisco J.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Balkcom, Kipling S.] ARS, USDA, Auburn, AL 36832 USA. RP Mourtzinis, S (reprint author), Auburn Univ, Dept Crop Soil & Environm Sci, 224 Funchess Hall, Auburn, AL 36849 USA. EM szm0020@tigermail.auburn.edu NR 13 TC 1 Z9 1 U1 1 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD MAY PY 2014 VL 6 IS 3 SI SI BP 300 EP 304 DI 10.1111/gcbb.12140 PG 5 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AE9YV UT WOS:000334369300014 ER PT J AU Ogbomo, SM Ayre, B Webber, CL D'Souza, NA AF Ogbomo, Sunny M. Ayre, Brian Webber, Charles L. D'Souza, Nandika A. TI Effect of kenaf fiber age on PLLA composite properties SO POLYMER COMPOSITES LA English DT Article ID REINFORCED POLYPROPYLENE COMPOSITES; MECHANICAL-PROPERTIES; CELLULOSIC FIBERS; POLY(L-LACTIDE); CRYSTALLIZATION; NANOCOMPOSITES; MATRIX AB A challenge facing engineering with natural fibers is the high standard deviation of mechanical properties of natural fiber compared with synthetic fiber. Plants have a chemical and physical architecture reflective of their age. The region near the apex is more flexible than that near the base. In this paper we investigate the impact of increasing age of plant fiber on the corresponding composite. Bast fibers stems of kenaf (Hibiscus cannabinus, L.), a warm season tropical herbaceous annual plant extracted corresponding to different age, were dispersed into Poly-l-lactide (PLLA) matrix by melt blending followed by compression molding. The resulting bio-based hybrid composites were characterized by X-ray diffraction (XRD), attenuated total reflectance-Fourier transfer infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), optical microscopy (OM), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA) were conducted. DSC and XRD indicated that the kenaf was effective in promoting crystallization. TGA indicated that the thermal stability of composites is reduced compared with PLLA, but the older fiber sample based on 120-150 cm from the plant apex improved thermal stability compared with the rest. SEM and OM inferred good fiber dispersion while dynamic mechanical tests revealed increased modulus. POLYM. COMPOS., 35:915-924, 2014. (c) 2013 Society of Plastics Engineers C1 [Ogbomo, Sunny M.; D'Souza, Nandika A.] Univ N Texas, Dept Mat Sci & Engn, Denton, TX 76203 USA. [Ayre, Brian] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA. [Webber, Charles L.] ARS, USDA, SRU, Houma, LA USA. [D'Souza, Nandika A.] Univ N Texas, Dept Mech & Energy Engn, Denton, TX 76203 USA. RP D'Souza, NA (reprint author), Univ N Texas, Dept Mat Sci & Engn, Denton, TX 76203 USA. EM ndsouza@unt.edu OI Ayre, Brian/0000-0002-7399-200X FU NSF [1114389, 1031828] FX Contract grant sponsor: NSF; contract grant numbers: 1114389, and 1031828. NR 42 TC 1 Z9 1 U1 0 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-8397 EI 1548-0569 J9 POLYM COMPOSITE JI Polym. Compos. PD MAY PY 2014 VL 35 IS 5 BP 915 EP 924 DI 10.1002/pc.22735 PG 10 WC Materials Science, Composites; Polymer Science SC Materials Science; Polymer Science GA AE4YZ UT WOS:000333996100011 ER PT J AU Whelan, G Kim, K Pelton, MA Soller, JA Castleton, KJ Molina, M Pachepsky, Y Zepp, R AF Whelan, Gene Kim, Keewook Pelton, Mitch A. Soller, Jeffrey A. Castleton, Karl J. Molina, Marirosa Pachepsky, Yakov Zepp, Richard TI An integrated environmental modeling framework for performing Quantitative Microbial Risk Assessments SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Integrated environmental modeling; QMRA; Risk assessment; Pathogens; Manure; Watershed modeling ID MULTIMEDIA BENCHMARKING ANALYSIS; SATURATED POROUS-MEDIA; ESCHERICHIA-COLI; DAIRY HERDS; REPRODUCIBLE RESEARCH; RECREATIONAL WATERS; SIMULATED RAINFALL; FECAL-COLIFORMS; MARINE WATER; TRANSPORT AB Standardized methods are often used to assess the likelihood of a human-health effect from exposure to a specified hazard, and inform opinions and decisions about risk management and communication. A Quantitative Microbial Risk Assessment (QMRA) is specifically adapted to detail potential human-health risks from exposure to pathogens; it can include fate and transport models for various media, including the source zone (initial fecal release), air, soil/land surface, surface water, vadose zone and aquifer. The analysis step of a QMRA can be expressed as a system of computer-based data delivery and modeling that integrates interdisciplinary, multiple media, exposure and effects models and databases. Although QMRA does not preclude using source-term and fate and transport models, it is applied most commonly where the source-term is represented by the receptor location (i.e., exposure point), so the full extent of exposure scenarios has not been rigorously modeled. An integrated environmental modeling infrastructure is, therefore, ideally suited to include fate and transport considerations and link the risk assessment paradigm between source and receptor seamlessly. A primary benefit of the source-to-outcome approach is that it allows an expanded view of relevant cause-and-effect relationships, which facilitate consideration of management options related to source terms and their fate and transport pathways. The Framework for Risk Analysis in Multimedia Environmental Systems (FRAMES) provides software technology for analysts to insert appropriate models and databases that fit the problem statement and design and construct QMRAs that are reproducible, flexible, transferable, reusable, and transparent. A sample application using different models and databases registered with FRAMES is presented. It illustrates how models are linked to assess six different manure-based contaminant sources, following three pathogens (Salmonella eterica, Cryptosporidium spp., and Escherichia coli O157:H7) to a receptor where exposures and health risk impacts are then evaluated. The modeling infrastructure demonstrates how analysts could use the system to discern which pathogens might be important and when, and which sources could contribute to their importance. Published by Elsevier Ltd. C1 [Whelan, Gene; Kim, Keewook; Molina, Marirosa; Zepp, Richard] US EPA, Off Res & Dev, Athens, GA 30605 USA. [Kim, Keewook] US DOE, Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. [Pelton, Mitch A.; Castleton, Karl J.] Pacific NW Natl Lab, Richland, WA 99352 USA. [Soller, Jeffrey A.] Soller Environm LLC, Berkeley, CA USA. [Pachepsky, Yakov] ARS, USDA, Beltsville, MD USA. RP Whelan, G (reprint author), US EPA, Off Res & Dev, Athens, GA 30605 USA. EM Whelan.Gene@epa.gov OI Kim, Keewook/0000-0002-6625-7285; Pachepsky, Yakov/0000-0003-0232-6090 FU U.S. Department of Energy; EPA FX The United States Environmental Protection Agency (EPA) through its Office of Research and Development collaborated with John Ravenscroft of the EPA Office of Water. It has been subjected to Agency review and approved for publication. This research was supported in part by an appointment to the Research Participation Program at the EPA Office of Research and Development, administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and EPA. NR 161 TC 6 Z9 6 U1 3 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD MAY PY 2014 VL 55 BP 77 EP 91 DI 10.1016/j.envsoft.2013.12.013 PG 15 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA AE5BW UT WOS:000334003800007 ER PT J AU Terefe, TG Visser, B Herselman, L Prins, R Negussie, T Kolmer, JA Pretorius, ZA AF Terefe, T. G. Visser, B. Herselman, L. Prins, R. Negussie, T. Kolmer, J. A. Pretorius, Z. A. TI Diversity in Puccinia triticina detected on wheat from 2008 to 2010 and the impact of new races on South African wheat germplasm SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Leaf rust; Lr34; Puccinia triticina; Race; Microsatellites ID F-SP TRITICI; LEAF RUST; PHYSIOLOGICAL SPECIALIZATION; UNITED-STATES; STEM RUST; RESISTANCE; PATHOGENICITY; VIRULENCE; MARKERS; UG99 AB Samples of wheat and triticale infected with leaf rust were collected from 2008 to 2010 in South Africa to identify Puccinia triticina races. Races were identified based on their virulence profile on standard differential lines. Eight races were identified from 362 isolates. The dominant races were 3SA133 (syn. PDRS) in 2008 (78 %) and 2009 (34 %), and 3SA145 (47 %) in 2010. Race 3SA145 (CCPS) identified in 2009 was a new race in South Africa with virulence for the adult plant resistance gene Lr37. Another new race, 3SA146 (MCDS), was identified in 2010. Race 3SA146 is also virulent for Lr37 but unlike 3SA145, it is virulent for Lr1 and Lr23 and avirulent for Lr3ka and Lr30. Microsatellite analysis showed that 3SA145 and 3SA146 shared 70 % genetic similarity with each other, but only 30 % similarity with other races in South Africa, suggesting that both represent foreign introductions. In seedling tests of 98 South African winter and spring cultivars and advanced breeding lines, 27 % were susceptible to 3SA145 and 3SA146 but resistant to 3SA133. In greenhouse studies of 59 spring wheat adult plants, 19 % of breeding lines and 46 % of cultivars were susceptible to 3SA145, whereas 29 % of the lines and 53 % of cultivars were susceptible to 3SA146. The cssfr6 gene-specific DNA marker confirmed the presence of Lr34 gene for leaf rust resistance in a homozygous condition in 28 wheat entries. Five entries were heterogeneous for Lr34. Several entries which were susceptible as seedlings to the new races carried Lr34. These lines are expected to show lower levels of leaf rust as adult plants. Results of these studies indicate a continued vulnerability of South African wheat cultivars to new races and emphasise the importance of regular rust monitoring and the need to incorporate genes for durable resistance. C1 [Terefe, T. G.] Agr Res Council, Small Grain Inst, ZA-9700 Bethlehem, South Africa. [Visser, B.; Herselman, L.; Prins, R.; Negussie, T.; Pretorius, Z. A.] Univ Orange Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. [Prins, R.] CenGen, ZA-6850 Worcester, South Africa. [Kolmer, J. A.] Univ Minnesota, USDA ARS Cereal Dis Lab, St Paul, MN 55108 USA. RP Terefe, TG (reprint author), Agr Res Council, Small Grain Inst, Private Bag X29, ZA-9700 Bethlehem, South Africa. EM terefet@arc.agric.za FU Winter Cereal Trust, South Africa FX Lizaan Rademeyer (CenGen), Debbie Snyman (CenGen) and Joyce Mebalo (ARC-SGI) are acknowledged for their technical assistance. This study was funded by the Winter Cereal Trust, South Africa. NR 26 TC 5 Z9 5 U1 1 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD MAY PY 2014 VL 139 IS 1 BP 95 EP 105 DI 10.1007/s10658-013-0368-3 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AE1AC UT WOS:000333696500010 ER PT J AU Taylor, JM King, RS Pease, AA Winemiller, KO AF Taylor, Jason M. King, Ryan S. Pease, Allison A. Winemiller, Kirk O. TI Nonlinear response of stream ecosystem structure to low-level phosphorus enrichment SO FRESHWATER BIOLOGY LA English DT Article DE fish; eutrophication; stoichiometry; attached algae; running waters/rivers/streams ID ECOLOGICAL COMMUNITY THRESHOLDS; WATER-QUALITY; FRESH-WATER; CATCHMENT URBANIZATION; OLIGOTROPHIC WETLAND; FLORIDA EVERGLADES; RISK-ASSESSMENT; STABLE-ISOTOPE; UNITED-STATES; STOICHIOMETRY AB Anthropogenic inputs of nitrogen (N) and phosphorus (P) create environmental conditions that alter biological organisation and ecosystem functioning in fresh waters. We studied 38 wadeable streams spanning a N and P gradient to contrast responses of algal and fish assemblages to nutrient enrichment. Surface water total P (TP) and total N (TN) concentrations represented a wide range (TP: 7-2380 mu gL(-1); TN: 127-15 860 mu gL(-1)) and were correlated across our study sites. Total P explained significantly more variance in periphyton carbon (C)-to-nutrient (C:P, C:N) and N:P ratios than TN. Abrupt, nonlinear declines in these ratios were observed between 20 and 50 mu gL(-1) TP and 500-1000 mu gL(-1) TN; beyond these values, ratios exhibited minimal additional decline. Algae assemblage structure was strongly linked to surface water TP, TN and catchment-scale nutrient sources (wastewater treatment plant (WWTP) discharges and % pasture cover). In particular, there were synchronous declines in frequency and cell densities of many alga species associated with TP concentrations >21 mu gL(-1) (90% CI of 18-48 mu gL(-1)) as well as simultaneous increases in tolerant species associated with increasing enrichment. Fish assemblage structure was most strongly associated with % pasture, WWTP discharges and fine sediment cover, yet also showed significant but weaker correlations with surface water and periphyton nutrient variables. However, two benthic fish species, Etheostoma spectabile and Campostoma anomalum, significantly declined with TP >28 mu gL(-1) (90% CI, 24-56 mu gL(-1)) and 34 mu gL(-1) (90% CI, 21-56 mu gL(-1)), respectively. Conversely, the tolerant minnow Cyprinella lutrensis and invasive carp Cyprinus carpio increased nonlinearly with increasing surface water TP. Our results provide new insights into interpretation and analysis of assemblage-level responses to nutrient enrichment. Our findings indicate that a numerical criterion for surface water TP of c. 20 mu gL(-1) would be needed to maintain natural algae assemblages and at least two specialist fishes within our study region. Proliferation of weedy alga species and increased abundance of invasive fishes are also likely when surface water concentrations exceed these thresholds. While many streams likely exceed these thresholds, managers should consider potential low-level enrichment effects when developing criteria for ecosystems to protect existing nutrient-limited streams. C1 [Taylor, Jason M.; King, Ryan S.] Baylor Univ, Dept Biol, Ctr Reservoir & Aquat Syst Res, Waco, TX 76798 USA. [Pease, Allison A.; Winemiller, Kirk O.] Texas A&M Univ, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. RP Taylor, JM (reprint author), ARS, Water Qual & Ecol Res Unit, Natl Sedimentat Lab, USDA, 598 McElroy Dr, Oxford, MS 38655 USA. EM jason.taylor@ars.usda.gov OI Winemiller, Kirk/0000-0003-0236-5129 FU TCEQ [582-7-83516]; U.S. Environmental Protection Agency's Science; U.S. Environmental Protection Agency's STAR program [FP-91694301-1] FX We thank M. Fisher and G. Easley of the Texas Commission on Environmental Quality (TCEQ) for their guidance and providing logistical support. Texas Parks and Wildlife Department provided collecting permits, and Baylor University and Texas Agrilife Research gave administrative support. For help collecting field data and samples, we thank C. Stanley, J. Grimm, E. Hooser, B. Kirchner, J. V. Montoya, C. Montana, W. Weise, Z. Johnson and P. Sims. J. Back provided analytical support for surface water and tissue nutrient concentrations through the Center for Reservoir and Aquatic System Research at Baylor University. B. Winsborough was contracted to perform all algal species identifications. Matthew Moore reviewed and provided useful comments that improved this paper. This research was supported through a TCEQ contract (Contract 582-7-83516) to RSK and KOW, but all conclusions and recommendations are solely by the authors and do not necessarily reflect those of the agency. Additionally, JMT was supported by a grant from the U.S. Environmental Protection Agency's Science to Achieve Results (STAR) Graduate Research Fellowship program. Although the research described in the article has been funded in part by the U.S. Environmental Protection Agency's STAR program through grant (FP-91694301-1), it has not been subjected to any EPA review and therefore does not necessarily reflect the views of the agency, and no official endorsement should be inferred. NR 64 TC 12 Z9 12 U1 7 U2 104 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0046-5070 EI 1365-2427 J9 FRESHWATER BIOL JI Freshw. Biol. PD MAY PY 2014 VL 59 IS 5 BP 969 EP 984 DI 10.1111/fwb.12320 PG 16 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA AE1AV UT WOS:000333698500007 ER PT J AU Levi, MR Rasmussen, C AF Levi, Matthew R. Rasmussen, Craig TI Covariate selection with iterative principal component analysis for predicting physical soil properties SO GEODERMA LA English DT Article DE Digital soil mapping; Regression kriging; Landsat; Spatial variability; Terrain analysis; Data reduction ID SPATIAL PREDICTION; SAMPLING STRATEGIES; TERRAIN ATTRIBUTES; REGRESSION; VARIABILITY; MODEL; MAPS; INFORMATION; SIMULATION; EQUATIONS AB Local and regional soil data can be improved by coupling new digital soil mapping techniques with high resolution remote sensing products to quantify both spatial and absolute variation of soil properties. The objective of this research was to advance data-driven digital soil mapping techniques for the prediction of soil physical properties at high spatial resolution using auxiliary data in a semiarid ecosystem in southeastern Arizona, USA. An iterative principal component analysis (iPCA) data reduction routine of reflectance and elevation covariate layers was combined with a conditioned Latin Hypercube field sample design to effectively capture the variability of soil properties across the 6250 ha study area. We sampled 52 field sites by genetic horizon to a 30 cm depth and determined particle size distribution, percent coarse fragments, Munsell color, and loss on ignition. Comparison of prediction models of surface soil horizons using ordinary kriging and regression kriging indicated that ordinary kriging had greater predictive power; however, regression kriging using principal components of covariate data more effectively captured the spatial patterns of soil property-landscape relationships. Percent silt and soil redness rating had the smallest normalized mean square error and the largest correlation between observed and predicted values, whereas soil coarse fragments were the most difficult to predict. This research demonstrates the efficacy of coupling data reduction, sample design, and geostatistical techniques for effective spatial prediction of soil physical properties in a semiarid ecosystem. The approach applied here is flexible and data-driven, allows incorporation of wide variety of numerically continuous covariates, and provides accurate quantitative prediction of individual soil properties for improved land management decisions and ecosystem and hydrologic models. Published by Elsevier B.V. C1 [Levi, Matthew R.; Rasmussen, Craig] Univ Arizona, Soil Water & Environm Sci Dept, Tucson, AZ 85721 USA. RP Levi, MR (reprint author), New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM mrlevi21@email.arizona.edu FU USDA-Natural Resources Conservation Service of Arizona [68-9457-8-466]; NSF EAR/IF [0929850]; Arizona Agricultural Experiment Station [ARZT-1367190-H21-155] FX This research was supported by the USDA-Natural Resources Conservation Service of Arizona, Cooperative Agreement #68-9457-8-466, NSF EAR/IF #0929850, and the Arizona Agricultural Experiment Station ARZT-1367190-H21-155. The authors would like to thank the handling editor and two anonymous reviewers for their helpful comments that greatly improved the manuscript. NR 75 TC 10 Z9 10 U1 2 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD MAY PY 2014 VL 219 BP 46 EP 57 DI 10.1016/j.geoderma.2013.12.013 PG 12 WC Soil Science SC Agriculture GA AD8BV UT WOS:000333492400006 ER PT J AU Reese, GC Wilson, KR Flather, CH AF Reese, Gordon C. Wilson, Kenneth R. Flather, Curtis H. TI Performance of species richness estimators across assemblage types and survey parameters SO GLOBAL ECOLOGY AND BIOGEOGRAPHY LA English DT Article DE community ecology; simulation; nonparametric estimator; selection framework; Biodiversity; sample coverage ID CAPTURE-RECAPTURE MODELS; SAMPLE COVERAGE; POPULATION-SIZE; NONPARAMETRIC-ESTIMATION; ABUNDANCE; NUMBER; COMMUNITY; PATTERNS; AREA; EXTRAPOLATION AB Aim A raw count of the species encountered across surveys usually underestimates species richness. Statistical estimators are often less biased. Nonparametric estimators of species richness are widely considered the least biased, but no particular estimator has consistently performed best. This is partly a function of estimators responding differently to assemblage-level factors and survey design parameters. Our objective was to evaluate the performance of raw counts and nonparametric estimators of species richness across various assemblages and with different survey designs. Location We used both simulated and published field data. Methods We evaluated the bias, precision and accuracy of raw counts and 13 nonparametric estimators using simulations that systematically varied assemblage characteristics (number of species, species abundance distribution, total number of individuals, spatial configuration of individuals and species detection probability), sampling effort and survey design. Results informed the development of an estimator selection framework that we evaluated with field data. Results When averaged across assemblages, most nonparametric estimators were less negatively biased than a raw count. Estimators based on the similarity of repeated subsets of surveys were most accurate and their accumulation curves appeared to reach asymptotes fastest. Number of species, species abundance distribution and effort had the largest effects on performance, ultimately by affecting the proportion of the species pool contained in a sample. Our estimator selection framework showed promising results when applied to field data. Main conclusions A raw count of the number of species in an area is far from the best estimate of true species richness. Nonparametric estimators are less biased. Newer largely unused, estimators perform better than more well known and longer established counterparts under certain conditions. Given that there is generally a trade-off between bias and precision, we believe that estimator variance, which is often not reported when presenting species richness estimates, should always be included. C1 [Reese, Gordon C.; Wilson, Kenneth R.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Flather, Curtis H.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Reese, GC (reprint author), Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. EM greese126@gmail.com RI Flather, Curtis/G-3577-2012 OI Flather, Curtis/0000-0002-0623-3126 NR 53 TC 8 Z9 8 U1 4 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1466-822X EI 1466-8238 J9 GLOBAL ECOL BIOGEOGR JI Glob. Ecol. Biogeogr. PD MAY PY 2014 VL 23 IS 5 BP 585 EP 594 DI 10.1111/geb.12144 PG 10 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA AD7BV UT WOS:000333417100007 ER PT J AU Bock, CH Gottwald, TR Graham, JH AF Bock, Clive H. Gottwald, Tim R. Graham, James H. TI A Comparison of Pathogen Isolation in Culture and Injection-infiltration Bioassay of Citrus Leaves for Detecting Xanthomonas citri subsp. citri SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE USA; viable bacteria; infection; citrus bacterial canker; Citrus ID AXONOPODIS PV.-CITRI; POLYMERASE-CHAIN-REACTION; EPIPHYTIC FITNESS; SPLASH DISPERSAL; BACTERIAL CANKER; LEAF SURFACES; PCR; DIAGNOSIS; SURVIVAL; PRIMERS AB Citrus canker [caused by Xanthomonas citri subsp. citri (Xcc)] can cause yield loss of susceptible citrus and result in trade restrictions of fresh fruit. For both regulatory purposes and epidemiological studies, accurate detection and quantification of viable inoculum are critical. Two accepted methods used to detect and quantify Xcc are injection-infiltration bioassay and culture, but these two methods have not been directly compared using field-obtained samples. The two methods were compared using washates of lesions taken from fruit, leaves and shoots in a commercial orchard in Florida in 2009-2010 and 2010-2011, with bioassay being the assumed standard. Despite some misclassifications, true positives (sensitivity) and true negatives (specificity) were the dominant classes using culture. False positives for lesions from shoots ranged from 13.1 to 21.4% in 2009-2010 and 2010-2011, respectively, and false positives for lesions from fruit and leaves ranged from 4.3 to 15.7%, in the two seasons, respectively. The false positive rate for culture compared with injection-infiltration bioassay was highest (0.16-0.55), due to more frequent recovery of Xcc by culture at <= 10(3) colony-forming units (CFU) Xcc per ml. The false negative rate was consistently lower (0.02-0.21), confirming that in only a few cases did culture fail to detect Xcc when it was present. The area under the curve for receiver operator characteristic analysis ranged from 0.80 to 0.97, confirming that culture provided an accurate diagnosis in most cases. There was a higher frequency of lesions from shoots with a CFU <= 10(3) Xcc compared with lesions from fruit or leaves, making culture more effective at detecting these. The data demonstrate that culture is a reliable way to detect and quantify Xcc compared with injection-infiltration bioassay, particularly when the CFU is <= 10(3) Xcc per ml. C1 [Bock, Clive H.] USDA ARS SEFTNRL, Byron, GA 31008 USA. [Gottwald, Tim R.] USDA ARS USHRL, Ft Pierce, FL 34945 USA. [Graham, James H.] Univ Florida, CREC, Lake Alfred, FL 33850 USA. RP Bock, CH (reprint author), USDA ARS SEFTNRL, 21 Dunbar Rd, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov FU Citrus Research and Development Council FX We appreciate financial support from the Citrus Research and Development Council and technical support from Reid Lewis, Debbie Flynn, Len Therrien and Sean Reif. NR 50 TC 0 Z9 0 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD MAY PY 2014 VL 162 IS 5 BP 291 EP 301 DI 10.1111/jph.12189 PG 11 WC Plant Sciences SC Plant Sciences GA AE0DA UT WOS:000333632500003 ER PT J AU Link, TI Lang, P Scheffler, BE Duke, MV Graham, MA Cooper, B Tucker, ML Van De Mortel, M Voegele, RT Mendgen, K Baum, TJ Whitham, SA AF Link, Tobias I. Lang, Patrick Scheffler, Brian E. Duke, Mary V. Graham, Michelle A. Cooper, Bret Tucker, Mark L. Van De Mortel, Martijn Voegele, Ralf T. Mendgen, Kurt Baum, Thomas J. Whitham, Steven A. TI The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID EXPRESSED SEQUENCE TAGS; PLANT-PATHOGENIC FUNGI; SOYBEAN RUST; SECRETED PROTEINS; ARABIDOPSIS-THALIANA; AVIRULENCE GENE; AMINO-ACIDS; FLAX RUST; FABAE; GENOME AB Haustoria of biotrophic rust fungi are responsible for the uptake of nutrients from their hosts and for the production of secreted proteins, known as effectors, which modulate the host immune system. The identification of the transcriptome of haustoria and an understanding of the functions of expressed genes therefore hold essential keys for the elucidation of fungus-plant interactions and the development of novel fungal control strategies. Here, we purified haustoria from infected leaves and used 454 sequencing to examine the haustorial transcriptomes of Phakopsora pachyrhizi and Uromyces appendiculatus, the causal agents of soybean rust and common bean rust, respectively. These pathogens cause extensive yield losses in their respective legume crop hosts. A series of analyses were used to annotate expressed sequences, including transposable elements and viruses, to predict secreted proteins from the assembled sequences and to identify families of candidate effectors. This work provides a foundation for the comparative analysis of haustorial gene expression with further insights into physiology and effector evolution. C1 [Link, Tobias I.; Voegele, Ralf T.] Univ Stuttgart Hohenheim, FG Phytopathol, Inst Phytomed, D-70599 Stuttgart, Germany. [Lang, Patrick; Mendgen, Kurt] Univ Konstanz, Fachbereich Biol, D-78457 Constance, Germany. [Scheffler, Brian E.; Duke, Mary V.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Graham, Michelle A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Cooper, Bret; Tucker, Mark L.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Van De Mortel, Martijn; Baum, Thomas J.; Whitham, Steven A.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. RP Whitham, SA (reprint author), Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. EM swhitham@iastate.edu OI Scheffler, Brian/0000-0003-1968-8952 FU Deutsche Forschungsgemeinschaft [VO595/3-1, VO595/3-2]; Iowa Soybean Association; Iowa State University Plant Sciences Institute; Hatch Act Fund; State of Iowa Fund; Iowa Agriculture and Home Economics Experiment Station, Ames, IA, USA [3708] FX We thank Dan Nettleton for assistance with statistical analyses, and Sarah Pierce, Yeunsook Lee and Michael Ernst for technical assistance. We thank Francismar Guimaraes, Andres Cernadas and Tarek Hewezi for helpful discussions. This work was supported by the Deutsche Forschungsgemeinschaft (VO595/3-1 and VO595/3-2 to RTV), the Iowa Soybean Association, the Iowa State University Plant Sciences Institute and Hatch Act and State of Iowa Funds. This is a journal paper of the Iowa Agriculture and Home Economics Experiment Station, Ames, IA, USA, project number 3708. NR 90 TC 22 Z9 22 U1 1 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD MAY PY 2014 VL 15 IS 4 BP 379 EP 393 DI 10.1111/mpp.12099 PG 15 WC Plant Sciences SC Plant Sciences GA AE1TT UT WOS:000333753800007 PM 24341524 ER PT J AU Siega-Riz, AM Del Campo, YE Kinlaw, A Reinhart, GA Allen, LH Shahab-Ferdows, S Heck, J Suchindran, CM Bentley, ME AF Siega-Riz, Anna M. Del Campo, Yanire Estrada Kinlaw, Alan Reinhart, Gregory A. Allen, Lindsay H. Shahab-Ferdows, Setareh Heck, Jeff Suchindran, Chirayath M. Bentley, Margaret E. TI Effect of Supplementation with a Lipid-Based Nutrient Supplement on the Micronutrient Status of Children Aged 6-18 Months Living in the Rural Region of Intibuca, Honduras SO PAEDIATRIC AND PERINATAL EPIDEMIOLOGY LA English DT Article DE lipid-based nutrient supplements; randomised controlled trial; micronutrients; prevention; child undernutrition; Honduras ID DENSE COMPLEMENTARY FOOD; USE THERAPEUTIC FOOD; BREAST-MILK INTAKE; TO-USE FOOD; HOME FORTIFICATION; FORTIFIED SPREADS; MALAWIAN CHILDREN; INFANTS; ZINC; GROWTH AB Background Lipid-based nutrient supplements (LNS) have been effective in the treatment of acute malnutrition among children. We evaluated the use of LNS supplementation for improving the micronutrient status of young children. Methods A 12-month randomised controlled trial was conducted among children aged 6-18 months living in Intibuca, Honduras. Communities (n = 18) were randomised into clusters matched by poverty indicators (9 intervention, n = 160 and 9 controls, n = 140). Intervention participants received LNS. All children received food vouchers and nutrition education. Primary outcomes included measures of micronutrient status: at baseline, 6 and 12 months' blood were collected for assessment of folate, iron, zinc, riboflavin, and vitamin B-12 status; haemoglobin was measured every 3 months; and dietary and anthropometry collected monthly. Longitudinal analyses were based on intent to treat and LNS adherence. Generalised estimating equations were used in the estimation of generalised linear regression models specified for the data. Results At 6-month follow-up, children in the intervention group had a lower proportion classified as deficient for B-12 (43.6%) compared with the control (67.7%; P = 0.03). The intervention group had a higher mean concentration for folate at 6 months (P = 0.06), and improvements continued through 12 months for folate (P = 0.002) and vitamin A deficiency (P = 0.03). This pattern of results, with improved significance, remained in subanalysis based on LNS adherence. Conclusion These data demonstrate that LNS improved select micronutrient status in young non-malnourished Honduran children. C1 [Siega-Riz, Anna M.; Kinlaw, Alan] Dept Epidemiol, Dayton, OH USA. [Siega-Riz, Anna M.; Del Campo, Yanire Estrada; Bentley, Margaret E.] Dept Nutr, Dayton, OH USA. [Reinhart, Gregory A.] Mathile Inst Adv Human Nutr, Dayton, OH USA. [Allen, Lindsay H.; Shahab-Ferdows, Setareh] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Heck, Jeff] Univ N Carolina, Mt Area Hlth Educ Ctr, Asheville, NC 28804 USA. [Suchindran, Chirayath M.] Univ N Carolina, Dept Biostat, Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. RP Siega-Riz, AM (reprint author), Gillings Sch Global Publ Hlth, 2105-A McGavran Greenberg Hall,CB 7435, Chapel Hill, NC 27599 USA. EM am_siegariz@unc.edu FU Mathile Institute for the Advancement of Human Nutrition; Department of Nutrition Obesity Research Center [DK56350] FX This work was supported by the Mathile Institute for the Advancement of Human Nutrition and The Department of Nutrition Obesity Research Center (Grant #DK56350). The authors would like to acknowledge the work of the US Project Director Hayley Holland and data analyst Howard Chen who contributed to the initial data analysis procedures, and Dr Ruben Martinez who facilitated implementation of the intervention within the community, the community members of this region, and the women and children of the study. The authors report no conflict of interest. NR 47 TC 8 Z9 8 U1 2 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-5022 EI 1365-3016 J9 PAEDIATR PERINAT EP JI Paediatr. Perinat. Epidemiol. PD MAY PY 2014 VL 28 IS 3 BP 245 EP 254 DI 10.1111/ppe.12117 PG 10 WC Public, Environmental & Occupational Health; Obstetrics & Gynecology; Pediatrics SC Public, Environmental & Occupational Health; Obstetrics & Gynecology; Pediatrics GA AE2KH UT WOS:000333801300008 PM 24628577 ER PT J AU Wang, XF Wang, YF Pineros, MA Wang, ZY Wang, WX Li, CY Wu, ZC Kochian, LV Wu, P AF Wang, Xiaofei Wang, Yifeng Pineros, Miguel A. Wang, Zhiye Wang, Wenxia Li, Changying Wu, Zhongchang Kochian, Leon V. Wu, Ping TI Phosphate transporters OsPHT1;9 and OsPHT1;10 are involved in phosphate uptake in rice SO PLANT CELL AND ENVIRONMENT LA English DT Article DE Oryza sativa L.; phosphate translocation ID ARABIDOPSIS-THALIANA; KINETIC-PROPERTIES; PLANT-CELLS; PHT1 FAMILY; GENE; PHOSPHORUS; EXPRESSION; ROOT; STARVATION; BARLEY AB We characterized the function of two rice phosphate (Pi) transporters: OsPHT1;9 (OsPT9) and OsPHT1;10 (OsPT10). OsPT9 and OsPT10 were expressed in the root epidermis, root hairs and lateral roots, with their expression being specifically induced by Pi starvation. In leaves, expression of the two genes was observed in both mesophyll and vasculature. High-affinity Km values for Pi transport of OsPT9 and OsPT10 were determined by yeast experiments and two-electrode voltage clamp analysis of anion transport in Xenopus oocytes expressing OsPT9 and OsPT10. Pi uptake and Pi concentrations in transgenic plants harbouring overexpressed OsPT9 and OsPT10 were determined by Pi concentration analysis and P-33-labelled Pi uptake rate analysis. Significantly higher Pi uptake rates in transgenic plants compared with wild-type plants were observed under both high-Pi and low-Pi solution culture conditions. Conversely, although no alterations in Pi concentration were found in OsPT9 or OsPT10 knockdown plants, a significant reduction in Pi concentration in both shoots and roots was observed in double-knockdown plants grown under both high- and low-Pi conditions. Taken together, our results suggest that OsPT9 and OsPT10 redundantly function in Pi uptake. C1 [Wang, Xiaofei; Wang, Yifeng; Wang, Zhiye; Wang, Wenxia; Li, Changying; Wu, Zhongchang; Wu, Ping] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China. [Wang, Xiaofei] Zhejiang A&F Univ, Coll Agr & Food Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Linan 311300, Peoples R China. [Pineros, Miguel A.; Kochian, Leon V.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. RP Wu, P (reprint author), Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China. EM clspwu@zju.edu.cn OI wang, yifeng/0000-0002-5187-3755; Kochian, Leon/0000-0003-3416-089X; Pineros, Miguel/0000-0002-7166-1848 FU China 973 Program [2011CB100300]; China Postdoctoral Science Foundation [2012M511376] FX This work was supported by the China 973 Program (2011CB100300) and China Postdoctoral Science Foundation Funded Project (2012M511376). NR 35 TC 15 Z9 20 U1 5 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD MAY PY 2014 VL 37 IS 5 BP 1159 EP 1170 DI 10.1111/pce.12224 PG 12 WC Plant Sciences SC Plant Sciences GA AE1CY UT WOS:000333705200011 PM 24344809 ER PT J AU McCulloh, KA Johnson, DM Meinzer, FC Woodruff, DR AF McCulloh, Katherine A. Johnson, Daniel M. Meinzer, Frederick C. Woodruff, David R. TI The dynamic pipeline: hydraulic capacitance and xylem hydraulic safety in four tall conifer species SO PLANT CELL AND ENVIRONMENT LA English DT Article DE hydraulic conductance; capacitance; conifers; safety margins; vulnerability curves ID AIR-SEEDING THRESHOLDS; PONDEROSA PINE TREES; DOUGLAS-FIR TREES; WOODY-PLANTS; FOREST TREES; STOMATAL CONDUCTANCE; LEAF HYDRAULICS; NATIVE EMBOLISM; WATER RELATIONS; GAS-EXCHANGE AB Recent work has suggested that plants differ in their relative reliance on structural avoidance of embolism versus maintenance of the xylem water column through dynamic traits such as capacitance, but we still know little about how and why species differ along this continuum. It is even less clear how or if different parts of a plant vary along this spectrum. Here we examined how traits such as hydraulic conductivity or conductance, xylem vulnerability curves, and capacitance differ in trunks, large- and small-diameter branches, and foliated shoots of four species of co-occurring conifers. We found striking similarities among species in most traits, but large differences among plant parts. Vulnerability to embolism was high in shoots, low in small- and large-diameter branches, and high again in the trunks. Safety margins, defined as the pressure causing 50% loss of hydraulic conductivity or conductance minus the midday water potential, were large in small-diameter branches, small in trunks and negative in shoots. Sapwood capacitance increased with stem diameter, and was correlated with stem vulnerability, wood density and latewood proportion. Capacitive release of water is a dynamic aspect of plant hydraulics that is integral to maintenance of long-distance water transport. C1 [McCulloh, Katherine A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Johnson, Daniel M.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Meinzer, Frederick C.; Woodruff, David R.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP McCulloh, KA (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM kmcculloh@wisc.edu RI Johnson, Daniel/E-6789-2011 OI Johnson, Daniel/0000-0001-5890-3147 FU NSF [IBN 09-19871] FX The authors are extremely grateful to the staff at the Wind River Field Station, particularly Matt Schroeder. We also thank Danielle Marias for help coring trees. KAM is also indebted to Adam Roddy and Tom Kursar for lengthy conversations about psychrometry. This work was supported by NSF grant IBN 09-19871. NR 70 TC 29 Z9 30 U1 9 U2 85 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD MAY PY 2014 VL 37 IS 5 BP 1171 EP 1183 DI 10.1111/pce.12225 PG 13 WC Plant Sciences SC Plant Sciences GA AE1CY UT WOS:000333705200012 PM 24289816 ER PT J AU Mascarin, GM Kobori, NN Vital, RCD Jackson, MA Quintela, ED AF Mascarin, Gabriel Moura Kobori, Nilce Naomi de Jesus Vital, Rayan Carlos Jackson, Mark Alan Quintela, Eliane Dias TI Production of microsclerotia by Brazilian strains of Metarhizium spp. using submerged liquid culture fermentation SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Ascomycetes; Biological control; Desiccation tolerance; Mycoinsecticide; Storage stability ID BIOLOGICAL-CONTROL; COLLETOTRICHUM-TRUNCATUM; WATER ACTIVITY; ANISOPLIAE; CONIDIA; FUNGI; CLAVICIPITACEAE; FORMULATION; EFFICACY; GRANULES AB We investigated the potential production and desiccation tolerance of microsclerotia (MS) by Brazilian strains of Metarhizium anisopliae (Ma), M. acridum (Mc) and M. robertsii (Mr). These fungi were grown in a liquid medium containing 16 g carbon l(-1) with a carbon:nitrogen ratio of 50:1. One hundred milliliters cultures were grown in 250 ml Erlenmeyer flasks in a rotary incubator shaker at 28 A degrees C and 200 rpm for 5 days. Five-day-old MS were harvested, mixed with diatomaceous earth (DE) and air-dried for 2 days at 30 A degrees C. The air-dried MS-DE granular preparations were milled by mortar + pestle and stored in centrifuged tubes at either 26 or -20 A degrees C. Desiccation tolerance and conidia production were assessed for dried MS granules by measuring hyphal germination after incubation for 2 days on water agar plates at 26 A degrees C and for conidia production following 7 days incubation. Yields of MS by all strains of Metarhizium were 6.1-7.3 x 10(6) l(-1) after 3 days growth with maximum MS yields (0.7-1.1 x 10(7) l(-1)) after 5 days growth. No differences in biomass accumulation were observed after 3 days growth, whereas Ma-CG168 showed the highest biomass accumulation after 5 days growth. Dried MS-DE preparations of all fungal strains were equally tolerant to desiccation (a parts per thousand yen93 % germination) and the highest conidia production was obtained by MS granules of Mc-CG423 (4 x 10(9) conidia g(-1)). All MS granules showed similar stability after storage at either 26 or -20 A degrees C for 3.5 months. C1 [Mascarin, Gabriel Moura; Kobori, Nilce Naomi; de Jesus Vital, Rayan Carlos; Quintela, Eliane Dias] Embrapa Rice & Beans, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Jackson, Mark Alan] ARS, Crop Bioprotect Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Mascarin, GM (reprint author), Embrapa Rice & Beans, Rodovia GO-462,Km 12,CP 179, BR-75375000 Santo Antonio De Goias, Go, Brazil. EM gabriel.mascarin@embrapa.br RI Mascarin, Gabriel/E-4646-2015 OI Mascarin, Gabriel/0000-0003-3517-6826 FU Embrapa (Empresa de Pesquisa Brasileria de Agropecuaria, Brasilia, Brazil) FX This work was funded by Embrapa (Empresa de Pesquisa Brasileria de Agropecuaria, Brasilia, Brazil). We thank Rogerio B. Lopes and Marcos R. Faria (Embrapa Cenargen) for providing the strains of Metarhizium spp. and for measuring water activities. NR 22 TC 2 Z9 3 U1 2 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD MAY PY 2014 VL 30 IS 5 BP 1583 EP 1590 DI 10.1007/s11274-013-1581-0 PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AD9YM UT WOS:000333620600016 PM 24343780 ER PT J AU Bradley, J Liu, HY Ravishankar, B Huang, L Perdue, A Urban, J McGaha, T AF Bradley, Jillian Liu, Haiyun Ravishankar, Buvana Huang, Lei Perdue, Aja Urban, Joseph McGaha, Tracy TI The role of GCN2 in type-2 mucosal inflammation within the lung SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Bradley, Jillian; Liu, Haiyun; Ravishankar, Buvana; Huang, Lei; Perdue, Aja; McGaha, Tracy] Georgia Regents Univ, GRU Canc Ctr, Canc Immunol Inflammat & Tolerance Program, Augusta, GA USA. [Urban, Joseph] ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA IRM7P.483 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765000108 ER PT J AU Heibel, S Chen, C Urban, J Dawson, H AF Heibel, Sandra Chen, Celine Urban, Joseph Dawson, Harry TI Effect of 1,25-dihydroxyvitamin Don all-trans retinoic acid metabolism and interleukin-4 signaling in porcine alternatively activated lung macrophages. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Heibel, Sandra; Chen, Celine; Urban, Joseph; Dawson, Harry] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA IRM7P.493 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765000112 ER PT J AU Hughes, H Vincent, A Loving, C AF Hughes, Holly Vincent, Amy Loving, Crystal TI Mucosal correlates of cross-protection for live-attenuated influenza virus vaccines in pigs SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Hughes, Holly; Vincent, Amy; Loving, Crystal] ARS, USDA, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1031 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006202 ER PT J AU Jarvi, S Pitt, W Osuna, A Farias, M Shiels, L Howe, K Jacquier, S Shiels, A Amano, K Luiz, B Maher, D Allison, M Holtguist, Z Scheibelhut, N Severino, M Riddick, L AF Jarvi, Susan Pitt, Will Osuna, Antonio Farias, Margaret Shiels, Laura Howe, Kay Jacquier, Steven Shiels, Aaron Amano, Karis Luiz, Blaine Maher, Daisy Allison, Maureen Holtguist, Zachariah Scheibelhut, Neil Severino, Michael Riddick, LaTasha TI Efficacy of vaccine for Angiostrongylus costaricensis against rat lungworm disease caused by A. cantonensis in wild Hawaiian Rats (Rattus rattus) SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Jarvi, Susan; Farias, Margaret; Shiels, Laura; Howe, Kay; Jacquier, Steven; Amano, Karis; Luiz, Blaine; Maher, Daisy; Allison, Maureen; Holtguist, Zachariah; Scheibelhut, Neil; Severino, Michael; Riddick, LaTasha] Univ Hawaii, Pharmaceut Sci, Hilo, HI 96720 USA. [Pitt, Will; Shiels, Laura; Shiels, Aaron] USDA APHIS, Wildlife Serv, NWRC Hawaii Field Stn, Hilo, HI USA. [Osuna, Antonio] Univ Granada, Parasitol, Granada, Spain. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VAC7P.978 PG 1 WC Immunology SC Immunology GA V44RB UT WOS:000209765002066 ER PT J AU Lee, SH Lillehoj, H Jeong, M Sullivan, YB AF Lee, Sung Hyen Lillehoj, Hyun Jeong, Misun Sullivan, Yvonne B. TI Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Lee, Sung Hyen; Lillehoj, Hyun; Jeong, Misun] ARS, USDA, Beltsville, MD USA. [Lee, Sung Hyen] RDA, Suwon, South Korea. [Sullivan, Yvonne B.] Kingfisher Biotech Inc, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1029 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006199 ER PT J AU Levings, R Roth, J AF Levings, Randall Roth, James TI Sequence and structural models of a bovine monoclonal antibody Fv region specific for bovine herpesvirus 1 glycoprotein D SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Levings, Randall] APHIS, Sci Serv, Vet Serv, USDA, Ames, IA USA. [Levings, Randall] APHIS, Technol Serv, Vet Serv, USDA, Ames, IA USA. [Levings, Randall] APHIS, Anal Serv, Vet Serv, USDA, Ames, IA USA. [Roth, James] Iowa State Univ, Coll Vet Med, Vet Microbiol & Prevent Med, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1037 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006196 ER PT J AU Lillehoj, H Lee, SH Dupuis, L Ben Arous, J AF Lillehoj, Hyun Lee, Sung Hyen Dupuis, Laurent Ben Arous, Juliette TI Evaluation of Montanide (TM) ISA 71 VG adjuvant against coccidiosis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Lillehoj, Hyun; Lee, Sung Hyen] ARS, USDA, Beltsville, MD USA. [Dupuis, Laurent; Ben Arous, Juliette] SEPPIC, Puteaux La Defense, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1030 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006201 ER PT J AU Liu, R Gutierrez, A Loving, C Vincent, A Moise, L Terry, F Martin, B Groot, A AF Liu, Rui Gutierrez, Andres Loving, Crystal Vincent, Amy Moise, Leonard Terry, Frances Martin, Bill Groot, Annie TI Design of T cell epitope-driven IAV vaccines for swine using immunoinformatics tools SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Liu, Rui; Gutierrez, Andres; Moise, Leonard; Groot, Annie] Univ Rhode Isl, Inst Immunol & Informat, Providence, RI 02908 USA. [Loving, Crystal; Vincent, Amy] ARS, Virus & Prion Dis Res Unit, NADC, USDA, Aimes, IA USA. [Moise, Leonard; Terry, Frances; Martin, Bill; Groot, Annie] EpiVax Inc, Providence, RI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VAC2P.929 PG 1 WC Immunology SC Immunology GA V44RB UT WOS:000209765002132 ER PT J AU Loying, C Vincent, A Gauger, P Hughes, H AF Loying, Crystal Vincent, Amy Gauger, Phillip Hughes, Holly TI Differential antiviral immune response in swine model of influenza vaccine-associated enhanced respiratory disease. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Loying, Crystal; Vincent, Amy; Hughes, Holly] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Gauger, Phillip] Iowa State Univ, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VIR2P.1026 PG 1 WC Immunology SC Immunology GA V44RB UT WOS:000209765005072 ER PT J AU Maggioli, MF Palmer, M Vordermeier, HM Whelan, A Waters, WR AF Maggioli, Mayara Fernanda Palmer, Mitchell Vordermeier, H. Martin Whelan, Adam Waters, Wade Ray TI Polyfunctional CD4 T cells in the response to bovine tuberculosis. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Maggioli, Mayara Fernanda] Iowa State Univ, IMBIO, Ames, IA USA. [Maggioli, Mayara Fernanda; Palmer, Mitchell; Waters, Wade Ray] Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA USA. [Vordermeier, H. Martin] Anim Hlth & Vet Labs Agcy Weybridge, TB Res Grp, New Haw, England. [Whelan, Adam] Anim Hlth & Vet Labs Agcy Weybridge, New Haw, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1034 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006206 ER PT J AU Maggioli, MF Palmer, M Vordermeier, HM Whelan, A Waters, WR AF Maggioli, Mayara Fernanda Palmer, Mitchell Vordermeier, H. Martin Whelan, Adam Waters, Wade Ray TI Bovine central memory T cells are highly proliferative in response to bovine tuberculosis infection. SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Maggioli, Mayara Fernanda] Iowa State Univ, Dept Vet Pathol, Ames, IA USA. [Maggioli, Mayara Fernanda; Palmer, Mitchell; Waters, Wade Ray] Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA USA. [Vordermeier, H. Martin] Anim Hlth & Vet Labs Agcy Weybridge, TB Res Grp, New Haw, England. [Whelan, Adam] Anim Hlth & Vet Labs Agcy Weybridge, New Haw, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA VET2P.1033 PG 2 WC Immunology SC Immunology GA V44RB UT WOS:000209765006204 ER PT J AU Ponichtera, H Shainheit, M Liu, BY Raychowdhury, R Larkin, B Russo, J Salantes, D Lai, CQ Parnell, L Yun, T Cheong, C Bunnell, S Hacohen, N Stadecker, M AF Ponichtera, Holly Shainheit, Mara Liu, Beiyun Raychowdhury, Raktima Larkin, Bridget Russo, Joanne Salantes, Danielle Lai, Chao-Qiang Parnell, Laurence Yun, Tae Cheong, Cheolho Bunnell, Stephen Hacohen, Nir Stadecker, Miguel TI Dendritic cell CD209a expression is critical for the development of pathogenic Th17 cell responses in murine schistosomiasis SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Ponichtera, Holly; Liu, Beiyun; Larkin, Bridget; Russo, Joanne; Salantes, Danielle; Bunnell, Stephen; Stadecker, Miguel] Tufts Univ, Sch Med, Dept Integrat Physiol & Pathobiol, Boston, MA 02111 USA. [Shainheit, Mara] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA. [Raychowdhury, Raktima; Hacohen, Nir] Broad Inst MIT & Harvard, Cambridge, MA USA. [Lai, Chao-Qiang; Parnell, Laurence] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Yun, Tae; Cheong, Cheolho] Inst Rech Clin Montreal, Montreal, PQ, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA INC9P.443 PG 1 WC Immunology SC Immunology GA V44RB UT WOS:000209765003049 ER PT J AU Sun, R Smith, A Desai, N Cheung, LM Grinchuk, V McLean, L Wang, AJ Urban, J Zhao, AP Shea-Donohue, T AF Sun, Rex Smith, Allen Desai, Neemesh Cheung, Lumei Grinchuk, Viktoriya McLean, Leon Wang, Anjiang Urban, Joseph Zhao, Aiping Shea-Donohue, Terez TI Neutrophil elastase regulates both type 1 and type 2 immune responses to enteric infection SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract C1 [Sun, Rex; Desai, Neemesh; Grinchuk, Viktoriya; McLean, Leon; Wang, Anjiang; Zhao, Aiping; Shea-Donohue, Terez] Univ Maryland, Med, Baltimore, MD 21201 USA. [Smith, Allen; Cheung, Lumei; Urban, Joseph] ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2014 VL 192 SU 1 MA MPF1P.800 PG 1 WC Immunology SC Immunology GA V44RB UT WOS:000209765001034 ER PT J AU Rogers, KD Cannistra, JC Gloer, JB Wicklow, DT AF Rogers, Kristina D. Cannistra, Joseph C. Gloer, James B. Wicklow, Donald T. TI Diplodiatoxin, chaetoglobosins, and diplonine associated with a field outbreak of Stenocarpella ear rot in Illinois SO MYCOTOXIN RESEARCH LA English DT Article DE Chaetoglobosins; Diplodiosis; Diplodiatoxin; Diplonine; Neuromycotoxicosis; Proline betaine (Stachydrine); Stenocarpella maydis; Zea mays ID PROLINE BETAINE; MAYDIS; MACROSPORA; MAIZE; METABOLITE; CULTURES; STRESS; GROWTH; CORN AB Stenocarpella maydis causes a fungal dry-rot of maize ears and is associated with diplodiosis, a neuromycotoxicosis in cattle grazing harvested maize fields in southern Africa and Argentina. There have been no reports of Stenocarpella metabolites in maize crop residues. Chemical investigations of S. maydis-infected grain from ears exhibiting different levels of ear rot severity following a 2010 field outbreak of Stenocarpella ear rot in Illinois led to the detection of diplodiatoxin and chaetoglobosins M and O as major components in the ethyl acetate extracts by LC-MS. Following post-harvest moist incubation of the S. maydis-infected grain, the amounts of each compound increased (approx. tenfold) and chaetoglobosin K was detected as a dominant toxin. In separate H-1 NMR-based analyses, the neurotoxin diplonine was detected as a minor component in methanol extracts of S. maydis-infected grain as well as cultures of S. maydis isolates from Midwest corn. Proline betaine (=stachydrine) and glycine betaine were also detected in these extracts as major components. This constitutes the first report of chaetoglobosin M, chaetoglobosin O, proline betaine, or glycine betaine from S. maydis, and the first record of diplodiatoxin, diplonine, proline betaine, glycine betaine, or chaetoglobosins M, O, or K being associated with a natural field outreak of S. maydis ear rot. C1 [Rogers, Kristina D.; Cannistra, Joseph C.; Gloer, James B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, Donald T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Wicklow, DT (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM donald.wicklow@ars.usda.gov FU National Science Foundation [CHE1011847] FX Support for this work from the National Science Foundation (CHE1011847) is gratefully acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture or the National Science Foundation. USDA is an equal opportunity provider and employer. NR 27 TC 3 Z9 3 U1 2 U2 5 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0178-7888 EI 1867-1632 J9 MYCOTOXIN RES JI Mycotoxin Res. PD MAY PY 2014 VL 30 IS 2 BP 61 EP 70 DI 10.1007/s12550-014-0188-0 PG 10 WC Mycology; Toxicology SC Mycology; Toxicology GA CS4WY UT WOS:000362078100001 PM 24504633 ER EF