FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Eck, E Wolfenbarger, S Ocamb, C Gent, D AF Eck, E. Wolfenbarger, S. Ocamb, C. Gent, D. TI Emergence and characterization of powdery mildew on hop cultivars with R6-based resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Eck, E.; Wolfenbarger, S.; Ocamb, C.] Oregon State Univ, Corvallis, OR 97331 USA. [Gent, D.] Oregon State Univ, USDA ARS, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 38 EP 38 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500206 ER PT J AU Elias-Roman, RD Guzman-Plazola, RA Alvarado-Rosales, D Calderon-Zavala, G Mora-Aguilera, JA Garcia-Espinosa, R Kim, MS Ross-Davis, AL Hanna, JW Klopfenstein, NB AF Elias-Roman, R. D. Guzman-Plazola, R. A. Alvarado-Rosales, D. Calderon-Zavala, G. Mora-Aguilera, J. A. Garcia-Espinosa, R. Kim, M. S. Ross-Davis, A. L. Hanna, J. W. Klopfenstein, N. B. TI Armillaria root disease in peach orchards of the state of Mexico, Mexico: Characterization of Armillaria species and assessment of disease impact SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Elias-Roman, R. D.; Guzman-Plazola, R. A.; Alvarado-Rosales, D.; Calderon-Zavala, G.; Mora-Aguilera, J. A.; Garcia-Espinosa, R.] Colegio Postgrad, Texcoco, Mexico. [Kim, M. S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. [Ross-Davis, A. L.; Hanna, J. W.; Klopfenstein, N. B.] USDA FS, Rocky Mt Res Stn, Moscow, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 39 EP 39 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500213 ER PT J AU Everhart, SE Larsen, MM Grunwald, NJ AF Everhart, S. E. Larsen, M. M. Grunwald, N. J. TI Where is Phytophthora ramorum now? An update on clonal populations in the US SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Everhart, S. E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Larsen, M. M.; Grunwald, N. J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 41 EP 41 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500225 ER PT J AU Feng, J Wang, M Hou, L Chen, X AF Feng, J. Wang, M. Hou, L. Chen, X. TI QTL mapping of resistance to stripe rust in spring wheat PI 182103 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Feng, J.; Wang, M.; Hou, L.] Washington State Univ, Pullman, WA 99164 USA. [Chen, X.] ARS, USDA, Pullman, WA USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 42 EP 43 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500233 ER PT J AU Fountain, JC Raruang, Y Luo, M Brown, RL Chen, ZY AF Fountain, J. C. Raruang, Y. Luo, M. Brown, R. L. Chen, Z. Y. TI The potential roles of WRKY transcription factors in regulating maize defense responses against Aspergillus flavus infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Fountain, J. C.; Raruang, Y.; Chen, Z. Y.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Luo, M.] Louisiana State Univ, Hlth Sci Ctr, New Orleans, LA USA. [Brown, R. L.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 1 Z9 1 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 45 EP 45 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500248 ER PT J AU Garber, NP Allen, J Cotty, PJ AF Garber, N. P. Allen, J. Cotty, P. J. TI Human exposure to aflatoxin in mesquite pod flour produced for personal consumption, cottage industry, and commercial markets in southeastern Arizona SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Garber, N. P.] Univ Arizona, Tucson, AZ USA. [Allen, J.] Aravaipa Heirlooms, Winkelman, AZ USA. [Cotty, P. J.] Univ Arizona, USDA ARS, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 47 EP 48 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500260 ER PT J AU Garcia, K Shaffer, JP Sarmiento, C Zalamea, PC Dalling, JW Davis, A Baltrus, DA Gallery, RE Arnold, AE AF Garcia, K. Shaffer, J. P. Sarmiento, C. Zalamea, P. C. Dalling, J. W. Davis, A. Baltrus, D. A. Gallery, R. E. Arnold, A. E. TI Diversity and evolutionary relationships of bacteria affiliated with tropical seeds and seed-associated fungi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Garcia, K.; Shaffer, J. P.; Baltrus, D. A.; Gallery, R. E.; Arnold, A. E.] Univ Arizona, Tucson, AZ USA. [Sarmiento, C.; Zalamea, P. C.] Smithsonian Trop Res Inst, Balboa, Ancon, Panama. [Dalling, J. W.] Univ Illinois, Urbana, IL USA. [Davis, A.] USDA ARS, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 48 EP 48 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500261 ER PT J AU Garcia, MO Luoma, DL Smith, JE AF Garcia, M. O. Luoma, D. L. Smith, J. E. TI Examining ectomycorrhizal communities in Pinus ponderosa and Pinus contorta in the Deschutes National Forest SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Garcia, M. O.; Luoma, D. L.] Oregon State Univ, Corvallis, OR 97331 USA. [Smith, J. E.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 48 EP 48 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500262 ER PT J AU Gevens, AJ Sanchez-Perez, A Halterman, D AF Gevens, A. J. Sanchez-Perez, A. Halterman, D. TI Novel clonal lineages of Phytophthora infestans elicit differential disease and pathogen responses on solanaceous hosts SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Gevens, A. J.; Sanchez-Perez, A.] Univ Wisconsin, Madison, WI USA. [Halterman, D.] USDA ARS, Madison, WI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 49 EP 49 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500268 ER PT J AU Gevens, AJ Sanchez-Perez, A Jansky, S Halterman, D AF Gevens, A. J. Sanchez-Perez, A. Jansky, S. Halterman, D. TI Development of diploid potato breeding lines with resistance to late blight caused by new clonal lineages of Phytophthora infestans SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Gevens, A. J.; Sanchez-Perez, A.] Univ Wisconsin, Madison, WI USA. [Jansky, S.] Univ Wisconsin, USDA ARS, Madison, WI 53706 USA. [Halterman, D.] USDA ARS, Madison, WI USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 49 EP 49 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500267 ER PT J AU Grisham, MP Haudenshield, JS Hoy, JW Glynn, N Comstock, J Hartman, GL AF Grisham, M. P. Haudenshield, J. S. Hoy, J. W. Glynn, N. Comstock, J. Hartman, G. L. TI Introduction of orange rust caused by Puccinia kuehnii into the Louisiana sugarcane industry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Grisham, M. P.] USDA ARS, Houma, LA USA. [Haudenshield, J. S.] USDA ARS, Urbana, IL USA. [Hoy, J. W.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Glynn, N.] Syngenta, Longmont, CO USA. [Comstock, J.] USDA ARS, Sugarcane Field Stn, Canal Point, FL USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 53 EP 53 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500289 ER PT J AU Grupe, A Henkel, T Baroni, T Lodge, DJ Smith, M AF Grupe, A. Henkel, T. Baroni, T. Lodge, D. J. Smith, M. TI Sarcodon in the neotropics-New species and the emerging circum-Caribbean distribution SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Grupe, A.; Henkel, T.] Humboldt State Univ, Arcata, CA 95521 USA. [Baroni, T.] SUNY Coll Cortland, Cortland, NY 13045 USA. [Lodge, D. J.] US Forest Serv, USDA, Loquillo, PR USA. [Smith, M.] Univ Florida, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 53 EP 53 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500290 ER PT J AU Guo, L Zhao, G Zhou, X Jonkers, W Kistler, CH Xu, JR Gao, L Ma, LJ AF Guo, L. Zhao, G. Zhou, X. Jonkers, W. Kistler, C. H. Xu, J. R. Gao, L. Ma, L. J. TI Gene regulatory network reconstruction in wheat pathogen Fusarium graminearum SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Guo, L.; Zhao, G.; Gao, L.; Ma, L. J.] Univ Massachusetts, Amherst, MA 01003 USA. [Zhou, X.; Xu, J. R.] Purdue Univ, W Lafayette, IN 47907 USA. [Jonkers, W.] Univ Calif Berkeley, Berkeley, CA 94720 USA. [Kistler, C. H.] ARS, USDA, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 1 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 53 EP 53 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500293 ER PT J AU Hammond, RW Hammond, J AF Hammond, R. W. Hammond, J. TI Evaluating the silencing suppressor activity of proteins encoded by Maize rayado fino virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hammond, R. W.] ARS, USDA, Beltsville, MD USA. [Hammond, J.] ARS, USDA, FNPRU, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 56 EP 56 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500306 ER PT J AU Hanson, LE Goodwill, TR McGrath, M AF Hanson, L. E. Goodwill, T. R. McGrath, M. TI Fusarium stalk blight and rot in sugar beet SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hanson, L. E.; Goodwill, T. R.; McGrath, M.] ARS, USDA, E Lansing, MI USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 56 EP 56 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500309 ER PT J AU Hebert, JB Marra, RE Hillman, BI Crouch, JA AF Hebert, J. B. Marra, R. E. Hillman, B. I. Crouch, J. A. TI Population genomic RAD-Seq characterization of the boxwood blight fungus, Calonectria pseudonaviculata SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hebert, J. B.] Rutgers State Univ, USDA ARS, Beltsville, MD USA. [Marra, R. E.] Connecticut Agr Expt Stn, New Haven, CT 06504 USA. [Hillman, B. I.] Rutgers State Univ, New Brunswick, NJ 08903 USA. [Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 58 EP 58 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500317 ER PT J AU Herrera, CS Rossman, AY Samuels, GJ Chaverri, P AF Herrera, C. S. Rossman, A. Y. Samuels, G. J. Chaverri, P. TI Phylogenetic analyses place Paranectria in the Nectriaceae SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Herrera, C. S.; Chaverri, P.] Univ Maryland, College Pk, MD 20742 USA. [Rossman, A. Y.; Samuels, G. J.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 59 EP 59 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500324 ER PT J AU Hewavitharana, SS Mazzola, M AF Hewavitharana, S. S. Mazzola, M. TI Carbon source-dependent efficacy of anaerobic soil disinfestation (ASD) in suppression of Rhizoctonia root rot of apple SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hewavitharana, S. S.] Washington State Univ, Wenatchee, WA USA. [Mazzola, M.] USDA ARS, Wenatchee, WA USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 60 EP 60 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500326 ER PT J AU Himmelstein, J Everts, K Maul, J Balci, Y AF Himmelstein, J. Everts, K. Maul, J. Balci, Y. TI General suppression of Fusarium wilt of watermelon via spring incorporated Vicia villosa and Trifolium incarnatum cover crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Himmelstein, J.; Everts, K.] Univ Maryland, Salisbury, MD USA. [Maul, J.] USDA ARS, Beltsville, MD USA. [Balci, Y.] Univ Maryland, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 60 EP 60 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500329 ER PT J AU Hong, J Martin, K Kokalis-Burelle, N Butler, D Rosskopf, E AF Hong, J. Martin, K. Kokalis-Burelle, N. Butler, D. Rosskopf, E. TI Bacterial population changes in fields treated with anaerobic soil disinfestation SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hong, J.; Kokalis-Burelle, N.; Rosskopf, E.] USDA ARS, Ft Pierce, FL USA. [Martin, K.] William Paterson Univ, Wayne, NJ USA. [Butler, D.] Univ Tennessee, Knoxville, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 61 EP 61 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500336 ER PT J AU Hughes, KW Petersen, RH Lodge, DJ Bergemann, SE Baumgartner, K Tulloss, RE Lickey, EB Cifuentes, J AF Hughes, K. W. Petersen, R. H. Lodge, D. J. Bergemann, S. E. Baumgartner, K. Tulloss, R. E. Lickey, E. B. Cifuentes, J. TI Evolutionary consequences of putative intra- and interspecific hybridization in agaric fungi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hughes, K. W.; Petersen, R. H.] Univ Tennessee, Knoxville, TN USA. [Lodge, D. J.] USDA Forest Serv, Luquillo, PR USA. [Bergemann, S. E.] Tennessee State Univ, Murfreesboro, TN USA. [Baumgartner, K.] Univ Calif Davis, Davis, CA 95616 USA. [Tulloss, R. E.] Herbarium Amanitarum Rooseveltensis, Roosevelt, NJ USA. [Lickey, E. B.] Bridgewater Coll, Bridgewater, VA USA. [Cifuentes, J.] Univ Mexico, Mexico City, DF, Mexico. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 63 EP 63 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500344 ER PT J AU Humann, R Gulya, T Marek, L Meyer, S Jordahl, J Markell, S AF Humann, R. Gulya, T. Marek, L. Meyer, S. Jordahl, J. Markell, S. TI Identification of sources of resistance to Plasmopara halstedii in wild annual sunflower (Helianthus annuus) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Humann, R.] N Dakota State Univ, Hazelton, ND USA. [Gulya, T.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Marek, L.] NCRPIS, Ames, IA USA. [Meyer, S.; Jordahl, J.; Markell, S.] N Dakota State Univ, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 63 EP 63 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500347 ER PT J AU Igwe, D Ngatat, S Cilia, M Bereman, MS MacCoss, MJ Gray, SM Hanna, R Kumar, L AF Igwe, D. Ngatat, S. Cilia, M. Bereman, M. S. MacCoss, M. J. Gray, S. M. Hanna, R. Kumar, L. TI Characterization of protein biomarkers linked to transmission competent and transmission refractive aphid and whitefly populations in Nigeria SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Igwe, D.; Kumar, L.] Int Inst Trop Agr, Ibadan, Nigeria. [Ngatat, S.; Hanna, R.] Int Inst Trop Agr, Yaounde, Cameroon. [Cilia, M.; Gray, S. M.] USDA ARS, Ithaca, NY 14853 USA. [Bereman, M. S.; MacCoss, M. J.] Univ Washington, Seattle, WA 98195 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 65 EP 65 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500356 ER PT J AU Jaime, R Cotty, PJ AF Jaime, R. Cotty, P. J. TI Temporal dynamic of Aspergillus flavus community structure in soils of fields treated with the atoxigenic biocontrol A. flavus AF36 in Arizona SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Jaime, R.] Univ Arizona, Sch Plant Sci, Tucson, AZ USA. [Cotty, P. J.] Univ Arizona, Sch Plant Sci, USDA ARS, Tucson, AZ USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 66 EP 66 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500364 ER PT J AU Janisiewicz, WJ Jurick, W Evans, B Peter, KA Bauchan, G Chao, CT AF Janisiewicz, W. J. Jurick, W. Evans, B. Peter, K. A. Bauchan, G. Chao, C. T. TI Wound response and ROS production in blue mold resistant wild apple germplasm from Kazakhstan and central Asia SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Janisiewicz, W. J.] USDA ARS, Kearneysville, WV USA. [Jurick, W.] USDA ARS, Food Qual Lab, Beltsville, MD USA. [Evans, B.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Peter, K. A.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Biglerville, PA USA. [Bauchan, G.] USDA ARS, Beltsville, MD USA. [Chao, C. T.] USDA ARS, Geneva, NY USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 67 EP 67 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500368 ER PT J AU Jin, X Abbas, HK Weaver, MA AF Jin, X. Abbas, H. K. Weaver, M. A. TI Formulation development of nontoxigenic biocontrol strain of Aspergillus flavus: Wetting agent selection and physical property comparison SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Jin, X.] ARS, USDA, MSA, Stoneville, MS USA. [Abbas, H. K.; Weaver, M. A.] ARS, USDA, MSA, BCPRU, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 68 EP 69 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500376 ER PT J AU Jurick, WM Vico, I Gaskins, VL Janisiewicz, WJ Evans, B Peter, KA AF Jurick, W. M. Vico, I. Gaskins, V. L. Janisiewicz, W. J. Evans, B. Peter, K. A. TI Resistance to postharvest fungicides in the blue mold fungus from the Mid-Atlantic area SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Jurick, W. M.] ARS, USDA, Food Qual Lab, Beltsville, MD USA. [Vico, I.] Univ Belgrade, Belgrade, Serbia. [Gaskins, V. L.] ARS, USDA, Beltsville, MD USA. [Janisiewicz, W. J.; Evans, B.] ARS, USDA, Kearneysville, WV USA. [Peter, K. A.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Biglerville, PA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 70 EP 70 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500385 ER PT J AU Kachapulula, PW Cotty, PJ Bandyopadhyay, R Dubois, T Mukanga, M AF Kachapulula, P. W. Cotty, P. J. Bandyopadhyay, R. Dubois, T. Mukanga, M. TI Etiology of aflatoxin contamination of maize in Zambia SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Kachapulula, P. W.] Univ Arizona, Tucson, AZ USA. [Cotty, P. J.] Univ Arizona, USDA ARS, Tucson, AZ USA. [Bandyopadhyay, R.; Dubois, T.] IITA, Ibadan, Nigeria. [Mukanga, M.] Zambia Agr Res Inst, Lusaka, Zambia. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 70 EP 70 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500386 ER PT J AU Kamvar, ZN Tabima, JF Grunwald, N AF Kamvar, Z. N. Tabima, J. F. Grunwald, N. TI The poppr R package for genetic analysis of populations with mixed (clonal/sexual) reproduction SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Kamvar, Z. N.; Tabima, J. F.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Grunwald, N.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 2 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 70 EP 70 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500387 ER PT J AU Kasson, MT O'Donnell, K Rooney, AP Sink, S Ploetz, RC Ploetz, JN Konkol, JL Carrillo, D Freeman, S Mendel, Z Smith, JA Black, AW Hulcr, J Bateman, C Stefkova, K Campbell, PR Geering, ADW Dann, EK Eskalen, A Mohotti, K Short, DPG Aoki, T Fenstermacher, KA Davis, DD Geiser, DM AF Kasson, M. T. O'Donnell, K. Rooney, A. P. Sink, S. Ploetz, R. C. Ploetz, J. N. Konkol, J. L. Carrillo, D. Freeman, S. Mendel, Z. Smith, J. A. Black, A. W. Hulcr, J. Bateman, C. Stefkova, K. Campbell, P. R. Geering, A. D. W. Dann, E. K. Eskalen, A. Mohotti, K. Short, D. P. G. Aoki, T. Fenstermacher, K. A. Davis, D. D. Geiser, D. M. TI An inordinate fondness for Fusarium: Phylogenetic diversity of fusaria cultivated by Euwallacea ambrosia beetles on avocado and other plant hosts SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Kasson, M. T.] Virginia Tech Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA USA. [O'Donnell, K.; Sink, S.] ARS, USDA, NCAUR, Bacterial Foodborne Pathogens & Mycol Unit, Peoria, IL USA. [Rooney, A. P.] ARS, USDA, NCAUR, Crop Bioprotect Res Unit, Peoria, IL USA. [Ploetz, R. C.; Ploetz, J. N.; Konkol, J. L.; Carrillo, D.] Univ Florida, IFAS, Ctr Trop Res & Educ, Homestead, FL 33031 USA. [Freeman, S.; Mendel, Z.] Agr Res Org, Volcani Ctr, Inst Plant Protect, IL-50250 Bet Dagan, Israel. [Smith, J. A.; Black, A. W.; Hulcr, J.; Bateman, C.; Stefkova, K.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Campbell, P. R.; Geering, A. D. W.; Dann, E. K.] Univ Queensland, Queensland Dept Agr Fisheries & Forestry, Dutton Pk, Australia. [Campbell, P. R.; Geering, A. D. W.; Dann, E. K.] Univ Queensland, Queensland Alliance Agr & Food Innovat, Dutton Pk, Australia. [Eskalen, A.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Mohotti, K.] Tea Res Inst Sri Lanka, Entomol & Nematol Div, Talawakelle, Sri Lanka. [Short, D. P. G.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA USA. [Aoki, T.] Natl Inst Agrobiol Sci, Genet Resources Ctr, Tsukuba, Ibaraki, Japan. [Fenstermacher, K. A.; Davis, D. D.; Geiser, D. M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RI Geering, Andrew/A-7912-2011; Geiser, David/J-9950-2013 NR 0 TC 0 Z9 0 U1 0 U2 38 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 71 EP 71 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500391 ER PT J AU Keller, KE Thomas, AL Quito-Avila, DF Martin, RR AF Keller, K. E. Thomas, A. L. Quito-Avila, D. F. Martin, R. R. TI Two distinct carlaviruses detected in elderberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Keller, K. E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. [Thomas, A. L.] Univ Missouri, Mt Vernon, MO USA. [Quito-Avila, D. F.] CIBE, ESPOL, Guaqayil, Ecuador. [Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 72 EP 72 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500395 ER PT J AU Keremane, ML Ramadugu, C Kubota, R Duan, YP Hall, DG Jenkins, D Lee, RF AF Keremane, M. L. Ramadugu, C. Kubota, R. Duan, Y. P. Hall, D. G. Jenkins, D. Lee, R. F. TI Development of a home detection kit for 'Candidatus Liberibacter asiaticus' (LAS) associated with citrus huanglongbing from psyllids SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Keremane, M. L.] USDA ARS, Riverside, CA USA. [Ramadugu, C.] Univ Calif Riverside, Riverside, CA 92521 USA. [Kubota, R.; Jenkins, D.] Univ Hawaii, Honolulu, HI 96822 USA. [Duan, Y. P.; Hall, D. G.] US Hort Res Lab, Ft Pierce, FL USA. [Lee, R. F.] USDA Natl Clonal Germplasm Repository Citrus & Da, Riverside, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 72 EP 73 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500398 ER PT J AU Keremane, ML Ramadugu, C Duan, YP Zhou, L Kund, G Trumble, J Lee, RF AF Keremane, M. L. Ramadugu, C. Duan, Y. P. Zhou, L. Kund, G. Trumble, J. Lee, R. F. TI Sequencing the metagenome of 'Candidatus Liberibacter psyllaurous' associated with tomato psyllid yellows using a BAC library SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Keremane, M. L.] ARS, USDA, Riverside, CA USA. [Ramadugu, C.; Kund, G.; Trumble, J.] Univ Calif Riverside, Riverside, CA 92521 USA. [Duan, Y. P.; Zhou, L.] US Hort Res Lab, Ft Pierce, FL USA. [Lee, R. F.] USDA Natl Clonal Germplasm Repository Citrus & Da, Riverside, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 72 EP 72 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500397 ER PT J AU Kim, MS Hohenlohe, PA Kim, KH Seo, ST Klopfenstein, NB AF Kim, M. S. Hohenlohe, P. A. Kim, K. H. Seo, S. T. Klopfenstein, N. B. TI Genetic diversity and population structure of the Korean oak wilt fungus (Raffaelea quercus-mongolicae) using RAD sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Kim, M. S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. [Hohenlohe, P. A.] Univ Idaho, Dept Biol Sci, Moscow, ID 83843 USA. [Hohenlohe, P. A.] Univ Idaho, Dept Stat, Moscow, ID 83843 USA. [Kim, K. H.; Seo, S. T.] Korea Forest Res Inst, Seoul, South Korea. [Klopfenstein, N. B.] US Forest Serv, USDA, RMRS, Moscow Forestry Sci Lab, Moscow, ID USA. NR 0 TC 0 Z9 0 U1 6 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 74 EP 74 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500405 ER PT J AU Kousik, CS Ikerd, JL AF Kousik, C. S. Ikerd, J. L. TI Confirming resistance in bottle gourd germplasm by quantifying powdery mildew conidia using a cellometer SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Kousik, C. S.; Ikerd, J. L.] USDA ARS, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 75 EP 75 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500412 ER PT J AU Lakshman, DK Amaradasa, BS Horvath, B AF Lakshman, D. K. Amaradasa, B. S. Horvath, B. TI Development of SCAR markers and UP-PCR cross-hybridization method for specific detection of brown patch pathogens from infected turfgrasses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lakshman, D. K.] USDA ARS, Beltsville, MD USA. [Amaradasa, B. S.] Univ Nebraska, Lincoln, NE USA. [Horvath, B.] Univ Tennessee, Knoxville, TN USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 76 EP 76 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500416 ER PT J AU Lan, P Li, F Abad, J Li, R AF Lan, P. Li, F. Abad, J. Li, R. TI Simultaneous detection and differentiation of three sweet potato potyviruses by one-step RT-PCR SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lan, P.; Li, R.] USDA ARS, Beltsville, MD USA. [Li, F.] Yunnan Agr Univ, Kunming, Peoples R China. [Abad, J.] USDA APHIS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 76 EP 76 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500418 ER PT J AU Larkin, RP Halloran, JM AF Larkin, R. P. Halloran, J. M. TI Effective implementation of disease-suppressive crops in potato rotations SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Larkin, R. P.; Halloran, J. M.] USDA ARS, Orono, ME USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 77 EP 77 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500424 ER PT J AU Lee, MW Tan, CC Rogers, EE Stenger, DC AF Lee, M. W. Tan, C. C. Rogers, E. E. Stenger, D. C. TI Functional characterization of two toxin-antitoxin systems of Xylella fastidiosa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lee, M. W.; Tan, C. C.; Rogers, E. E.; Stenger, D. C.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 78 EP 78 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500429 ER PT J AU Lee, S Singh, P Jia, MH Jia, Y AF Lee, S. Singh, P. Jia, M. H. Jia, Y. TI Analysis of rice PDR-like ABC transporter genes in sheath blight resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Lee, S.] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR USA. [Singh, P.] Govt India, Dept Biotechnol, Technol Adv Unit TAU, Lausanne, Switzerland. [Jia, M. H.; Jia, Y.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 78 EP 78 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500430 ER PT J AU Li, R Ling, KS AF Li, R. Ling, K. S. TI Molecular characterization and detection of Mexican papita viroid SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Li, R.; Ling, K. S.] ARS, USDA, US Vegetable Lab, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 80 EP 80 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500442 ER PT J AU Li, S Rupe, J Holland, R Steger, A Chen, P Sun, S Sciumbato, G AF Li, S. Rupe, J. Holland, R. Steger, A. Chen, P. Sun, S. Sciumbato, G. TI Evaluating soybean germplasm and commercial varieties for resistance to Phomopsis seed decay SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Li, S.] ARS, USDA, CGRU, Stoneville, MS USA. [Rupe, J.; Holland, R.; Steger, A.; Chen, P.; Sun, S.] Univ Arkansas, Fayetteville, AR 72701 USA. [Sciumbato, G.] Mississippi State Univ, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 80 EP 80 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500439 ER PT J AU Liang, M Deng, X Wang, X Chen, J AF Liang, M. Deng, X. Wang, X. Chen, J. TI Genomic and SEM characterization of a phytoplasma detected in huanglongbing-affected citrus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Liang, M.; Deng, X.] South China Agr Univ, Guangzhou, Guangdong, Peoples R China. [Wang, X.] South West Univ, Qiongqing, Peoples R China. [Chen, J.] ARS, USDA, PWA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 81 EP 82 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500448 ER PT J AU Liu, J Xu, L Guo, J Chen, R Que, Y Grisham, MP AF Liu, J. Xu, L. Guo, J. Chen, R. Que, Y. Grisham, M. P. TI Development of loop-mediated isothermal amplification for detection of Leifsonia xyli subsp xyli in sugarcane SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Liu, J.; Xu, L.; Guo, J.; Chen, R.; Que, Y.] Minist Agr, Key Lab Sugarcane Genet Improvement, Fuzhou, Peoples R China. [Grisham, M. P.] ARS, USDA, Houma, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 84 EP 84 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500466 ER PT J AU Liu, M Kolmer, J AF Liu, M. Kolmer, J. TI Population divergence in the wheat leaf rust fungus Puccinia triticina is correlated with wheat evolution SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Liu, M.] ECORC, AAFC, Ottawa, ON, Canada. [Kolmer, J.] ARS, USDA, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 84 EP 84 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500465 ER PT J AU Lodge, DJ Aime, MC Nakasone, KK AF Lodge, D. J. Aime, M. C. Nakasone, K. K. TI The tropical tree pathogen, Rhizomorpha corynephora (Agaricales, Marasmiaceae), is transported by birds in Belize SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lodge, D. J.] US Forest Serv, USDA, Luquillo, PR USA. [Aime, M. C.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Nakasone, K. K.] US Forest Serv, USDA, Madison, WI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 85 EP 85 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500469 ER PT J AU Longway, LJ Smith, JE Luoma, DL AF Longway, L. J. Smith, J. E. Luoma, D. L. TI Investigations of ectomycorrhizal communities of Chrysolepis chrysophylla & Pinus ponderosa along a soil moisture gradient SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Longway, L. J.; Luoma, D. L.] Oregon State Univ, Corvallis, OR 97331 USA. [Smith, J. E.] USDA, PNW Res Stn, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 85 EP 85 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500471 ER PT J AU Ma, J Jia, MH Jia, Y AF Ma, J. Jia, M. H. Jia, Y. TI Molecular characterization of rice blast resistance genes using a recombinant inbred population derived from a cross of Nipponbare and 93-11 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ma, J.] Univ Arkansas, Rice Res Extens Ctr, Stuttgart, AR USA. [Jia, M. H.; Jia, Y.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 88 EP 88 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500485 ER PT J AU Manamgoda, DS Rossman, AY Castlebury, LA Chukeatirote, E Lei, C Hyde, KD AF Manamgoda, D. S. Rossman, A. Y. Castlebury, L. A. Chukeatirote, E. Lei, C. Hyde, K. D. TI A taxonomic and phylogenetic study of the plant pathogenic genus Bipolaris SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Manamgoda, D. S.; Castlebury, L. A.] USDA ARS SMML, Beltsville, MD USA. [Rossman, A. Y.] USDA ARS, Beltsville, MD USA. [Chukeatirote, E.; Hyde, K. D.] Mae Fah Luang Univ, Chiang Rai, Thailand. [Lei, C.] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 89 EP 89 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500491 ER PT J AU Marra, RE Crouch, JA Hebert, JB Hillman, BI AF Marra, R. E. Crouch, J. A. Hebert, J. B. Hillman, B. I. TI Molecular diagnostics for the boxwood blight fungus, Calonectria pseudonaviculata: Strategies for early detection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Marra, R. E.] Connecticut Agr Expt Stn, New Haven, CT 06504 USA. [Crouch, J. A.] USDA ARS, Beltsville, MD USA. [Hebert, J. B.; Hillman, B. I.] Rutgers State Univ, New Brunswick, NJ 08903 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 90 EP 90 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500496 ER PT J AU Martin, RR Peres, NA Whidden, AJ AF Martin, R. R. Peres, N. A. Whidden, A. J. TI Virus outbreak in several Nova Scotia strawberry nurseries affects fruit growers in the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Martin, R. R.] USDA ARS, Corvallis, OR USA. [Peres, N. A.] Univ Florida, Wimauma, FL USA. [Whidden, A. J.] Univ Florida, Seffner, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 90 EP 91 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500499 ER PT J AU Martin, FN Douhan, GW Grunwald, NJ Coffey, MD AF Martin, F. N. Douhan, G. W. Grunwald, N. J. Coffey, M. D. TI Evaluating the correlation between mitochondrial haplotype and nuclear genotype of Phytophthora cinnamomi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Martin, F. N.] USDA ARS, Salinas, CA USA. [Douhan, G. W.; Coffey, M. D.] Univ Calif Riverside, Riverside, CA 92521 USA. [Grunwald, N. J.] USDA ARS, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 91 EP 91 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500500 ER PT J AU Mathew, F Castlebury, L Jordahl, J Gulya, T Markell, S AF Mathew, F. Castlebury, L. Jordahl, J. Gulya, T. Markell, S. TI Comparison of inoculation methods for characterizing aggressiveness of Phomopsis stem canker pathogens of sunflower SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Mathew, F.; Jordahl, J.; Markell, S.] N Dakota State Univ, Fargo, ND 58105 USA. [Castlebury, L.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. [Gulya, T.] ARS, USDA, No Crop Sci Lab, Fargo, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 91 EP 92 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500504 ER PT J AU Mazzola, M Strauss, SL AF Mazzola, M. Strauss, S. L. TI Changes in the apple rhizosphere microbiome associated with orchard system resilience conferred by Brassicaceae seed meal amendment SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Mazzola, M.; Strauss, S. L.] ARS, USDA, Wenatchee, WA USA. RI Strauss, Sarah/I-5141-2016 OI Strauss, Sarah/0000-0001-7494-5320 NR 0 TC 0 Z9 0 U1 1 U2 15 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 92 EP 93 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500510 ER PT J AU Mccann, T Grove, G Mahaffee, W Gadoury, DM Cadle-Davidson, L Gadoury, DC Seem, RC AF Mccann, T. Grove, G. Mahaffee, W. Gadoury, D. M. Cadle-Davidson, L. Gadoury, D. C. Seem, R. C. TI Ontogenesis of conidiation in the grapevine powdery mildew (Erysiphe necator) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Mccann, T.] Univ Florida, Gainesville, FL USA. [Grove, G.] Washington State Univ, Prosser, WA USA. [Mahaffee, W.] ARS, USDA, Corvallis, OR USA. [Gadoury, D. M.; Gadoury, D. C.; Seem, R. C.] Cornell Univ, Geneva, NY USA. [Cadle-Davidson, L.] ARS, USDA, Geneva, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 93 EP 93 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500512 ER PT J AU McCotter, SW Humann, J Main, D Wildung, MR Castlebury, LA Carris, LM AF McCotter, S. W. Humann, J. Main, D. Wildung, M. R. Castlebury, L. A. Carris, L. M. TI A next generation sequencing approach for the identification and annotation of the mating locus in the wheat bunt pathogen Tilletia caries SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [McCotter, S. W.; Carris, L. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Humann, J.; Main, D.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Wildung, M. R.] Washington State Univ, Lab Bioanal & Biotechnol, Pullman, WA 99164 USA. [Castlebury, L. A.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 93 EP 93 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500513 ER PT J AU Medeiros, FHV Souza, RM Medeiros, FCL Zhang, HM Wheeler, T Payton, P Ferro, HM Pare, PW AF Medeiros, F. H. V. Souza, R. M. Medeiros, F. C. L. Zhang, H. M. Wheeler, T. Payton, P. Ferro, H. M. Pare, P. W. TI Unraveling Bacillus subtilis induced tolerance to damping-off in cotton SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Medeiros, F. H. V.; Souza, R. M.; Ferro, H. M.] Univ Fed Lavras, Lavras, Brazil. [Medeiros, F. C. L.] Syngenta Protecao Cultivos Ltda, Lavras, Brazil. [Zhang, H. M.] Purdue Univ, W Lafayette, IN 47907 USA. [Wheeler, T.] Texas AgriLife, Lubbock, TX USA. [Payton, P.] USDA, Lubbock, TX USA. [Pare, P. W.] Texas Tech Univ, Lubbock, TX 79409 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 94 EP 94 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500519 ER PT J AU Mehl, HL Cotty, PJ AF Mehl, H. L. Cotty, P. J. TI Use of quantitative pyrosequencing to dissect complex pathogen-pathogen interactions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Mehl, H. L.] Univ Arizona, Tucson, AZ USA. [Cotty, P. J.] Univ Arizona, USDA, ARS, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 94 EP 94 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500521 ER PT J AU Miller-Butler, MA Curry, KJ Kreiser, BR Smith, BJ AF Miller-Butler, M. A. Curry, K. J. Kreiser, B. R. Smith, B. J. TI Screening strawberry (Fragaria x ananassa ) germplasm for anthracnose disease resistance using traditional techniques and molecular markers SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Miller-Butler, M. A.; Smith, B. J.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS USA. [Curry, K. J.; Kreiser, B. R.] Univ So Mississippi, Hattiesburg, MS 39406 USA. NR 0 TC 0 Z9 0 U1 1 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 97 EP 97 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500536 ER PT J AU Misner, I Pandey, R Roberts, DP Alkharouf, N Pain, A Lakshman, DK AF Misner, I. Pandey, R. Roberts, D. P. Alkharouf, N. Pain, A. Lakshman, D. K. TI Comparative genomic analyses of Rhizoctonia solani: Insights on evolution and pathogenesis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Misner, I.] Towson Univ, USDA, ARS, Beltsville, MD USA. [Pandey, R.; Lakshman, D. K.] ARS, USDA, Floral & Nursery Plants Res Unit, Beltsville, MD USA. [Roberts, D. P.] ARS, USDA, Sustainable Agr Syst Lab, Beltsville, MD USA. [Alkharouf, N.] Town Univ, Dept Comp & Informat Sci, Towson, MD USA. [Pain, A.] King Abdullah Univ Sci & Technol, Thuwal, Saudi Arabia. RI Pain, Arnab/L-5766-2015 OI Pain, Arnab/0000-0002-1755-2819 NR 0 TC 1 Z9 1 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 98 EP 98 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500540 ER PT J AU Moore, GG AF Moore, G. G. TI Letting that love light shine: A study of the sexuality of eGFP-transformed Aspergillus flavus biocontrol strains SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Moore, G. G.] ARS, USDA, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 99 EP 99 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500547 ER PT J AU Moran-Diez, ME Crutcher, FK Krieger, I Kenerley, CM AF Moran-Diez, M. E. Crutcher, F. K. Krieger, I. Kenerley, C. M. TI Characterization of a novel protein from Trichoderma virens with chitinolytic activity and a role in mycoparasitism SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Moran-Diez, M. E.; Kenerley, C. M.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Crutcher, F. K.] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX USA. [Krieger, I.] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 99 EP 99 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500549 ER PT J AU Nagabhyru, P Dinkins, RD Bacon, CW Schardl, CL AF Nagabhyru, P. Dinkins, R. D. Bacon, C. W. Schardl, C. L. TI Effect of endophyte on tall fescue host plant metabolite levels and gene expression under drought stress SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Nagabhyru, P.; Schardl, C. L.] Univ Kentucky, Lexington, KY USA. [Dinkins, R. D.] ARS, USDA, Forage Anim Prod Res Unit, Lexington, KY USA. [Bacon, C. W.] ARS, USDA, Toxicol & Mycotoxin Res Unit, Athens, GA USA. RI Schardl, Christopher/P-5655-2014 OI Schardl, Christopher/0000-0003-2197-0842 NR 0 TC 0 Z9 0 U1 1 U2 17 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 101 EP 101 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500559 ER PT J AU Ngatat, S Hanna, R Kumar, L Gray, SM Cilia, M Fontem, DA AF Ngatat, S. Hanna, R. Kumar, L. Gray, S. M. Cilia, M. Fontem, D. A. TI Long term evaluation of the susceptibility of 16 Musa genotypes to banana bunchy top disease in Cameroon, Central Africa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ngatat, S.; Hanna, R.] Int Inst Trop Agr, Yaounde, Cameroon. [Kumar, L.] Int Inst Trop Agr, Ibadan, Nigeria. [Gray, S. M.; Cilia, M.] ARS, USDA, Ithaca, NY USA. [Fontem, D. A.] Univ Dschang, Fac Agr, Dept Plant Protect, Dschang, Cameroon. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 103 EP 103 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500566 ER PT J AU Nwugo, C Venkatesan, V Munyaneza, J Lin, H AF Nwugo, C. Venkatesan, V. Munyaneza, J. Lin, H. TI Transcriptomic, proteomic, and nutritional analyses of potato tissues infected with 'Candidatus Liberibacter solanacearum' SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Nwugo, C.; Lin, H.] ARS, USDA, Parlier, CA USA. [Venkatesan, V.; Munyaneza, J.] ARS, USDA, Austin, TX USA. NR 0 TC 0 Z9 0 U1 2 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 104 EP 104 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500575 ER PT J AU Nyczepir, AP Meyer, SLF Brannen, PM AF Nyczepir, A. P. Meyer, S. L. F. Brannen, P. M. TI Management of Meloidogyne incognita with tall fescue grass rotations prior to peach orchard establishment SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Nyczepir, A. P.] ARS, USDA, Byron, GA USA. [Meyer, S. L. F.] ARS, USDA, Beltsville, MD USA. [Brannen, P. M.] Univ Georgia, Athens, GA 30602 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 104 EP 105 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500576 ER PT J AU Ogunbayo, T Callicott, K Probst, C Cotty, P AF Ogunbayo, T. Callicott, K. Probst, C. Cotty, P. TI An atoxigenic vegetative compatibility group of Aspergillus flavus widely adapted to maize production in Africa and North America SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ogunbayo, T.; Callicott, K.; Cotty, P.] ARS, USDA, Tucson, AZ USA. [Probst, C.] Washington State Univ, Richland, WA USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 105 EP 106 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500582 ER PT J AU Ohm, R Goodwin, S Grigoriev, I AF Ohm, R. Goodwin, S. Grigoriev, I. CA Dothideomycetes Consortium TI Diverse lifestyles and strategies of plant pathogenesis encoded in the genomes of eighteen Dothideomycetes fungi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ohm, R.; Grigoriev, I.] US DOE, Joint Genome Inst, Walnut Creek, CA USA. [Goodwin, S.] Purdue Univ, USDA, ARS, W Lafayette, IN 47907 USA. NR 0 TC 2 Z9 2 U1 5 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 106 EP 106 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500583 ER PT J AU Olarte, RA Horn, BW Worthington, CJ Heiniger, RW Lewis, MH Ojiambo, PS Singh, R Carbone, I AF Olarte, R. A. Horn, B. W. Worthington, C. J. Heiniger, R. W. Lewis, M. H. Ojiambo, P. S. Singh, R. Carbone, I. TI Population structure of Aspergillus flavus before and after biocontrol treatment SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Olarte, R. A.; Worthington, C. J.; Heiniger, R. W.; Lewis, M. H.; Ojiambo, P. S.; Singh, R.; Carbone, I.] N Carolina State Univ, Raleigh, NC 27695 USA. [Horn, B. W.] ARS, Natl Peanut Res Lab, USDA, Dawson, GA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 106 EP 106 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500584 ER PT J AU Oliver, AK Brown, SP Callaham, M Jumpponen, A AF Oliver, A. K. Brown, S. P. Callaham, M. Jumpponen, A. TI Ectomycorrhizal community responses to recurring prescribed fires in yellow pine forests: Effects of fire intervals and season SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Oliver, A. K.; Brown, S. P.] Kansas State Univ, Manhattan, KS 66506 USA. [Callaham, M.] US Forest Serv, USDA, Athens, GA USA. [Jumpponen, A.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. NR 0 TC 1 Z9 1 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 107 EP 107 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500589 ER PT J AU Ortega-Beltran, A Callicott, KA Grubisha, LC Cotty, PJ AF Ortega-Beltran, A. Callicott, K. A. Grubisha, L. C. Cotty, P. J. TI Variability in the aflatoxin biosynthesis gene cluster among members of the atoxigenic Aspergillus flavus VCG YV36 endemic to Mexico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ortega-Beltran, A.] Univ Arizona, Tucson, AZ USA. [Callicott, K. A.; Cotty, P. J.] ARS, USDA, Tucson, AZ USA. [Grubisha, L. C.] Ctr Coll Danville, Danville, KY USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 108 EP 108 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500595 ER PT J AU Ortiz-Santana, B Lindner, DL Miettinen, O Justo, A Hibbett, DS AF Ortiz-Santana, B. Lindner, D. L. Miettinen, O. Justo, A. Hibbett, D. S. TI Phylogeny and species delimitation in the genus Antrodia (Polyporales, Basidiomycota) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ortiz-Santana, B.; Lindner, D. L.] US Forest Serv, No Res Stn, CFMR, Madison, WI USA. [Miettinen, O.] Univ Helsinki, Bot Museum, Helsinki, Finland. [Justo, A.] Clark Univ, Worcester, MA 01610 USA. [Hibbett, D. S.] Clark Univ, Dept Biol, Worcester, MA 01610 USA. OI Miettinen, Otto/0000-0001-7502-710X NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 108 EP 108 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500596 ER PT J AU Ostry, ME Jacobs, JJ Moore, MJ Anderson, NA AF Ostry, M. E. Jacobs, J. J. Moore, M. J. Anderson, N. A. TI Histopathology of bronze leaf disease of Populus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ostry, M. E.; Moore, M. J.; Anderson, N. A.] US Forest Serv, USDA, St Paul, MN USA. [Jacobs, J. J.] US Forest Serv, USDA, Forest Hlth, Albuquerque, NM USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 108 EP 109 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500598 ER PT J AU Peterson, S Labeda, D AF Peterson, S. Labeda, D. TI A multilocus database for the identification of Aspergillus and Penicillium species SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Peterson, S.; Labeda, D.] ARS, USDA, NCAUR, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 113 EP 113 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500621 ER PT J AU Ploetz, R Ploetz, J Konkol, J O'Donnell, K Campbell, A Duncan, R AF Ploetz, R. Ploetz, J. Konkol, J. O'Donnell, K. Campbell, A. Duncan, R. TI Fusarium symbionts of an ambrosia beetle (Euwallacea sp.) in southern Florida are pathogens of avocado, Persea americana SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Ploetz, R.; Ploetz, J.; Konkol, J.; Duncan, R.] Univ Florida, Homestead, FL USA. [O'Donnell, K.] ARS, USDA, MWA, NCAUR, Peoria, IL USA. [Campbell, A.] Univ Florida, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 41 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 114 EP 114 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500629 ER PT J AU Polashock, J Oudemans, PV Smolinski, T AF Polashock, J. Oudemans, P. V. Smolinski, T. TI A next-generation sequencing approach to identifying the causal agent of funky flower in cranberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Polashock, J.] ARS, USDA, Chatsworth, NJ USA. [Oudemans, P. V.] Rutgers State Univ, Chatsworth, NJ USA. [Smolinski, T.] Delaware State Univ, Dover, DE USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 114 EP 114 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500630 ER PT J AU Polashock, J Johnson-Cicalese, J Vorsa, N AF Polashock, J. Johnson-Cicalese, J. Vorsa, N. TI Characterization of fruit rot resistance and inheritance in American cranberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Polashock, J.] ARS, USDA, Chatsworth, NJ USA. [Johnson-Cicalese, J.; Vorsa, N.] Rutgers State Univ, Chatsworth, NJ USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 114 EP 114 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500626 ER PT J AU Power, IL Arias, RS Culbreath, AK AF Power, I. L. Arias, R. S. Culbreath, A. K. TI Genetic polymorphism of Puccinia arachidis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Power, I. L.; Culbreath, A. K.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Arias, R. S.] ARS, USDA, Natl Peanut Res Lab, Dawson, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 115 EP 115 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500636 ER PT J AU Pu, L Li, R Bateman, M Kinard, G AF Pu, L. Li, R. Bateman, M. Kinard, G. TI Molecular characterization of six new Asian prunus virus isolates: Evidences of their recombination and high genetic diversity SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Pu, L.; Li, R.; Kinard, G.] ARS, USDA, Beltsville, MD USA. [Bateman, M.] USDA APHIS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 116 EP 116 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500639 ER PT J AU Quito-Avila, DF Peralta, EL Ibarra, MA Alvarez, RA Martin, RR AF Quito-Avila, D. F. Peralta, E. L. Ibarra, M. A. Alvarez, R. A. Martin, R. R. TI A new Raspberry bushy dwarf virus isolate from Ecuador exhibits an aberrant genetic feature SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Quito-Avila, D. F.; Peralta, E. L.; Ibarra, M. A.; Alvarez, R. A.] CIBE ESPOL, Guayaquil, Ecuador. [Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 117 EP 117 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500646 ER PT J AU Quito-Avila, DF Martin, RR Ibarra, MA Alvarez, RA Espinoza, L Peralta, EL AF Quito-Avila, D. F. Martin, R. R. Ibarra, M. A. Alvarez, R. A. Espinoza, L. Peralta, E. L. TI Severe symptoms on bell pepper plants infected with Bell pepper endornavirus and Cucumber mosaic virus suggest a synergistic effect between the viruses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Quito-Avila, D. F.; Ibarra, M. A.; Alvarez, R. A.; Espinoza, L.; Peralta, E. L.] CIBE ESPOL, Guayaquil, Ecuador. [Martin, R. R.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 117 EP 117 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500645 ER PT J AU Ramadugu, C Keremane, ML Halbert, SE Stover, E Duan, YP Lee, RF AF Ramadugu, C. Keremane, M. L. Halbert, S. E. Stover, E. Duan, Y. P. Lee, R. F. TI A field trial to evaluate HLB tolerance and resistance in Citrus and citrus relatives SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ramadugu, C.] Univ Calif Riverside, Riverside, CA 92521 USA. [Keremane, M. L.] USDA Natl Clonal Germplasm Repository Citrus & Da, Riverside, CA USA. [Halbert, S. E.] Div Plant Ind, Gainesville, FL USA. [Stover, E.; Duan, Y. P.] US Hort Res Lab, Ft Pierce, FL USA. [Lee, R. F.] ARS, USDA, Riverside, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 118 EP 118 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500650 ER PT J AU Rapicavoli, JN Clifford, J Shugart, H Backus, E May, C Perring, T Roper, MC AF Rapicavoli, J. N. Clifford, J. Shugart, H. Backus, E. May, C. Perring, T. Roper, M. C. TI The role of the bacterial cell surface lipopolysaccharide in grapevine colonization and insect acquisition of Xylella fastidiosa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Rapicavoli, J. N.; May, C.; Perring, T.; Roper, M. C.] Univ Calif Riverside, Riverside, CA 92521 USA. [Clifford, J.] USDA ARS, Corvallis, OR USA. [Shugart, H.; Backus, E.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 119 EP 119 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500653 ER PT J AU Raudabaugh, DB Lindner, DL Porras-Alfaro, A Miller, AN AF Raudabaugh, D. B. Lindner, D. L. Porras-Alfaro, A. Miller, A. N. TI Nutritional capability and substrate suitability of psychrophilic species of Geomyces from the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Raudabaugh, D. B.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Lindner, D. L.] US Forest Serv, No Res Stn, Ctr Forest Mycol Res, Madison, WI USA. [Porras-Alfaro, A.] Western Illinois Univ, Dept Biol Sci, Macomb, IL 61455 USA. [Miller, A. N.] Univ Illinois, Illinois Nat Hist Survey, Champaign, IL 61820 USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 119 EP 119 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500654 ER PT J AU Reed, S Juzwik, J English, J AF Reed, S. Juzwik, J. English, J. TI Fungi isolated from four ambrosia beetle species emerged from stressed black walnut SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Reed, S.; English, J.] Univ Missouri, Columbia, MO USA. [Juzwik, J.] US Forest Serv, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 120 EP 120 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500660 ER PT J AU Rioux, R Willis, DK Bent, A Kerns, J AF Rioux, R. Willis, D. K. Bent, A. Kerns, J. TI Expression of germin-like protein genes in response to Sclerotinia homoeocarpa infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Rioux, R.; Willis, D. K.; Bent, A.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Willis, D. K.] USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. [Kerns, J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 121 EP 121 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500666 ER PT J AU Roberts, DP McKenna, LF Kobayashi, D Lakshman, D Mattoo, A Misner, I Loper, J AF Roberts, D. P. McKenna, L. F. Kobayashi, D. Lakshman, D. Mattoo, A. Misner, I. Loper, J. TI A vfr homologue of Pseudomonas protegens Pf-5 regulates antibiotic production and traits important to root colonization SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Roberts, D. P.; Lakshman, D.; Mattoo, A.] USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [McKenna, L. F.] ARS, USDA, Beltsville, MD USA. [Kobayashi, D.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. [Misner, I.] Towson Univ, Towson, MD USA. [Loper, J.] USDA, Hort Crops Res Lab, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 121 EP 122 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500669 ER PT J AU Rong, X Paul, PA Schisler, DA Gardener, BBM AF Rong, X. Paul, P. A. Schisler, D. A. Gardener, B. B. McSpadden TI Characterization and field detection of Cryptococcus flavescens strains, biocontrol agents against Fusarium head blight of wheat SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Rong, X.; Paul, P. A.; Gardener, B. B. McSpadden] Ohio State Univ, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Schisler, D. A.] ARS, USDA, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 123 EP 123 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500677 ER PT J AU Ross-Davis, AL Stewart, JE Shaw, JD Kim, MS Klopfenstein, NB AF Ross-Davis, A. L. Stewart, J. E. Shaw, J. D. Kim, M. S. Klopfenstein, N. B. TI Metagenomic approaches for surveying forest soil microbial communities on permanent plots SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Ross-Davis, A. L.; Klopfenstein, N. B.] US Forest Serv, USDA, RMRS, Moscow Forestry Sci Lab, Moscow, ID USA. [Stewart, J. E.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Shaw, J. D.] US Forest Serv, USDA, RMRS, Ogden, UT USA. [Kim, M. S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. NR 0 TC 0 Z9 0 U1 3 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 123 EP 123 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500678 ER PT J AU Ross-Davis, AL Graca, RN Alfenas, AC Peever, TL Hanna, JW Uchida, JY Hauff, RD Kadooka, CY Kim, MS Cannon, PG Namba, S Minato, N Simento, S Perez, CA Rayamajhi, MB Moran, M Lodge, DJ Arguedas, M Medel-Ortiz, R Lopez-Ramirez, MA Tennant, P Glen, M Klopfenstein, NB AF Ross-Davis, A. L. Graca, R. N. Alfenas, A. C. Peever, T. L. Hanna, J. W. Uchida, J. Y. Hauff, R. D. Kadooka, C. Y. Kim, M. S. Cannon, P. G. Namba, S. Minato, N. Simento, S. Perez, C. A. Rayamajhi, M. B. Moran, M. Lodge, D. J. Arguedas, M. Medel-Ortiz, R. Lopez-Ramirez, M. A. Tennant, P. Glen, M. Klopfenstein, N. B. TI Tracking the distribution of the pandemic Puccinia psidii genotypes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Ross-Davis, A. L.; Hanna, J. W.; Klopfenstein, N. B.] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID USA. [Graca, R. N.; Alfenas, A. C.] Univ Fed Vicosa, Dept Fitopatol, BR-36570 Vicosa, MG, Brazil. [Peever, T. L.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Uchida, J. Y.; Kadooka, C. Y.] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Dept Plant & Environm Protect Sci, Honolulu, HI 96822 USA. [Hauff, R. D.] DLNR, Div Forestry & Wildlife, Honolulu, HI USA. [Kim, M. S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. [Cannon, P. G.] US Forest Serv, USDA, Vallejo, CA USA. [Namba, S.; Minato, N.] Univ Tokyo, Fac Agr, Grad Sch Agr & Life Sci, Lab Plant Pathol,Dept Agr & Environm Biol, Tokyo 113, Japan. [Simento, S.] INIA Tacuarembo, Programa Forestal, Tacuarembo, Uruguay. [Perez, C. A.] Univ Republ, Fac Agron, EEMAC, Dept Protecc Vegetal, Paysandu, Uruguay. [Rayamajhi, M. B.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. [Moran, M.] Desarrollos Madereros SA, Hernandarias, Paraguay. [Lodge, D. J.] US Forest Serv, USDA, No Res Stn, Luquillo, PR USA. [Arguedas, M.] Inst Tecnol Costa Rica, Escuela Ingn Forestal, Cartago, Costa Rica. [Medel-Ortiz, R.; Lopez-Ramirez, M. A.] Univ Veracruzana, Inst Invest Forestales, Xalapa 91000, Veracruz, Mexico. [Tennant, P.] Univ W Indies, Ctr Biotechnol, Kingston 7, Jamaica. [Glen, M.] Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas, Australia. RI Alfenas, Acelino/H-9504-2014 OI Alfenas, Acelino/0000-0001-9947-5847 NR 0 TC 0 Z9 0 U1 0 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 123 EP 124 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500679 ER PT J AU Rotich, F Feng, C Jia, Y Correll, J AF Rotich, F. Feng, C. Jia, Y. Correll, J. TI Characterizing virulence phenotypes among U. S. isolates of Pyricularia oryzae using IRRI NILs, US germplasm, and NERICA lines SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Rotich, F.; Feng, C.; Correll, J.] Univ Arkansas, Fayetteville, AR 72701 USA. [Jia, Y.] ARS, USDA, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 124 EP 124 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500681 ER PT J AU Roy, A Choudhary, N Otero-Colina, G Wei, G Stone, A Achor, D Shao, J Levy, L Nakhla, MK Hollingsworth, CR Hartung, JS Schneider, W Brlansky, RH AF Roy, A. Choudhary, N. Otero-Colina, G. Wei, G. Stone, A. Achor, D. Shao, J. Levy, L. Nakhla, M. K. Hollingsworth, C. R. Hartung, J. S. Schneider, W. Brlansky, R. H. TI Complete genome sequence of nuclear citrus leprosis utilizing small RNA deep sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Roy, A.; Choudhary, N.; Achor, D.; Brlansky, R. H.] Univ Florida, Lake Alfred, FL USA. [Otero-Colina, G.] Colegio Postgrad, Montecillo, Texcoco, Mexico. [Wei, G.; Nakhla, M. K.] USDA APHIS PPQ CPHST, Beltsville, MD USA. [Stone, A.; Schneider, W.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. [Shao, J.; Hartung, J. S.] ARS, USDA, MPPL, Beltsville, MD USA. [Levy, L.] USDA APHIS PPQ CPHST, Riverdale, MD USA. [Hollingsworth, C. R.] USDA APHIS PPQ CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 124 EP 125 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500680 ER PT J AU RoyChowdhury, M Gross, DC Lin, H AF RoyChowdhury, M. Gross, D. C. Lin, H. TI Functional characterization of virulence genes of 'Candidatus Liberibacter solanacearum', bacterium associated with potato zebra chip (ZC) disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [RoyChowdhury, M.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [RoyChowdhury, M.] ARS, USDA, CDPG, San Joaquin Valley Agr Sci Ctr, College Stn, TX USA. [Gross, D. C.] Texas A&M Univ, College Stn, TX USA. [Lin, H.] ARS, USDA, CDPG, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 125 EP 125 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500687 ER PT J AU Salgado-Salazar, C Hirooka, Y Rossman, AY Zhuang, WY Chaverri, P AF Salgado-Salazar, C. Hirooka, Y. Rossman, A. Y. Zhuang, W. Y. Chaverri, P. TI Preliminary studies of biogeography, genetic diversity and host range of the causal agents of beech bark disease (BBD) and related species SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Salgado-Salazar, C.; Chaverri, P.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Hirooka, Y.] Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 305, Japan. [Rossman, A. Y.] ARS, USDA, Beltsville, MD USA. [Zhuang, W. Y.] Chinese Acad Sci, Key Lab Systemat Mycol & Lichenol Lab, Inst Microbiol, Beijing, Peoples R China. NR 0 TC 0 Z9 0 U1 3 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 125 EP 126 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500691 ER PT J AU Salgado-Salazar, C Rossman, AY Chaverri, P AF Salgado-Salazar, C. Rossman, A. Y. Chaverri, P. TI Molecular phylogeny of fungi in the genus Thelonectria (Hypocreales, Nectriaceae): Are they really monophyletic? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Salgado-Salazar, C.] Univ Maryland, College Pk, MD 20742 USA. [Rossman, A. Y.] ARS, USDA, Beltsville, MD USA. [Chaverri, P.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 125 EP 125 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500690 ER PT J AU Sayler, R Bluhm, B Cary, J Rajasekaran, K Jaynes, J AF Sayler, R. Bluhm, B. Cary, J. Rajasekaran, K. Jaynes, J. TI Transgenic approaches to control aflatoxins in maize SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Sayler, R.; Bluhm, B.] Univ Arkansas, Fayetteville, AR 72701 USA. [Cary, J.; Rajasekaran, K.] USDA, Food & Feed Safety Res Unit, New Orleans, LA USA. [Jaynes, J.] Tuskegee Univ, Tuskegee, AL 36088 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 127 EP 127 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500699 ER PT J AU Schisler, DA Paul, PA Boehm, MJ Bradley, CA Dunlap, CA AF Schisler, D. A. Paul, P. A. Boehm, M. J. Bradley, C. A. Dunlap, C. A. TI Antagonist Cryptococcus flavescens OH 182.9 3C colonization of wheat heads when applied with triazole fungicides and the effect on scab SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Schisler, D. A.; Dunlap, C. A.] ARS, USDA, MWA, NCAUR, Peoria, IL USA. [Paul, P. A.] Ohio State Univ, OARDC, Wooster, OH USA. [Boehm, M. J.] Ohio State Univ, Columbus, OH 43210 USA. [Bradley, C. A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 127 EP 128 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500702 ER PT J AU Schmidt, LS Browne, GT AF Schmidt, L. S. Browne, G. T. TI Characterization of Pythium species associated with Prunus replant disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Schmidt, L. S.; Browne, G. T.] ARS, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 128 EP 128 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500705 ER PT J AU Schuenzel, E Scally, M Braswell, E AF Schuenzel, E. Scally, M. Braswell, E. TI The detection of 'Candidatus Liberibacter asiaticus' in worldwide populations of Diaphorina citri SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Schuenzel, E.; Scally, M.] Univ Texas Pan Amer, Edinburg, TX 78541 USA. [Braswell, E.] USDA APHIS, Mission, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 129 EP 129 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500710 ER PT J AU Gounder, VS Buchman, JL Henne, DC Pappu, HR Munyaneza, JE AF Gounder, V. Sengoda Buchman, J. L. Henne, D. C. Pappu, H. R. Munyaneza, J. E. TI 'Candidatus Liberibacter solanacearum' titer over time in the potato psyllid, Bactericera cockerelli, following acquisition from infected plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Gounder, V. Sengoda; Munyaneza, J. E.] ARS, USDA, Wapato, WA USA. [Buchman, J. L.] N Dakota State Univ, Fargo, ND 58105 USA. [Henne, D. C.] Texas AgriLife Res Weslaco, Weslaco, TX USA. [Pappu, H. R.] Washington State Univ, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 130 EP 130 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500717 ER PT J AU Serrato-Diaz, L Rivera-Vargas, LI Goenaga, R French-Monar, RD AF Serrato-Diaz, L. Rivera-Vargas, L. I. Goenaga, R. French-Monar, R. D. TI Identification of the fungal pathogen complex causing fruit rot of rambutan (Nephelium lappaceum L.) in Puerto Rico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Serrato-Diaz, L.; French-Monar, R. D.] Texas A&M AgriLife Extens Serv, Amarillo, TX USA. [Rivera-Vargas, L. I.] Univ Puerto Rico, Mayaguez, PR USA. [Goenaga, R.] ARS, USDA, Mayaguez, PR USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 130 EP 130 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500718 ER PT J AU Sexton, Z Hughes, T AF Sexton, Z. Hughes, T. TI Greenhouse evaluation of commercial soybean varieties adapted to the North Central US for resistance to charcoal rot SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Sexton, Z.] Purdue Univ, New City, NY USA. [Hughes, T.] ARS, USDA, W Lafayette, IN USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 130 EP 130 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500719 ER PT J AU Poudyal, DS Paulitz, T Porter, L Eggers, J Hamm, PB du Toit, L AF Poudyal, D. Sharma Paulitz, T. Porter, L. Eggers, J. Hamm, P. B. du Toit, L. TI Rhizoctonia spp. dynamics and optimal timing of glyphosate application to cereal cover crops to manage onion stunting in Washington and Oregon SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Poudyal, D. Sharma] Washington State Univ, Pullman, WA 99164 USA. [Paulitz, T.] ARS, USDA, Pullman, WA USA. [Porter, L.] ARS, USDA, Prosser, WA USA. [Eggers, J.; Hamm, P. B.] Oregon State Univ, Hermiston, OR USA. [du Toit, L.] Washington State Univ, Mt Vernon, WA USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 131 EP 131 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500724 ER PT J AU Shi, X Tian, L Lin, H AF Shi, X. Tian, L. Lin, H. TI Characterization of Xylella fastidiosa popP gene required for pathogenicity SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Shi, X.; Tian, L.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Lin, H.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 132 EP 132 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500727 ER PT J AU Shin, SH Song, Q Cregan, PB Pastor-Corrales, MA AF Shin, S. H. Song, Q. Cregan, P. B. Pastor-Corrales, M. A. TI Simple sequence repeat DNA markers linked with broad-spectrum rust resistance in common bean PI 310762 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Shin, S. H.; Song, Q.; Pastor-Corrales, M. A.] USDA ARS, SGI Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. [Cregan, P. B.] USDA ARS, SGI Lab, Beltsville Agr Res Ctr, Beltsville, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 132 EP 132 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500729 ER PT J AU Shishkoff, N AF Shishkoff, N. TI The concentration of sporangia or zoospores of Phytophthora ramorum required for infection of host roots SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Shishkoff, N.] USDA ARS FDWSRU, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 132 EP 133 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500730 ER PT J AU Sikdar, P Mazzola, M Xiao, CL AF Sikdar, P. Mazzola, M. Xiao, C. L. TI Phacidiopycnis washingtonensis: Inoculum availability, persistence and seasonal host susceptibility in Washington apple orchards SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Sikdar, P.] Washington State Univ, TFREC, Wenatchee, WA USA. [Mazzola, M.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 133 EP 133 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500734 ER PT J AU Skaltsas, DN Castlebury, L Chaverri, P AF Skaltsas, D. N. Castlebury, L. Chaverri, P. TI Delimitation of tropical endophytic Diaporthe species from three euphorbiaceous hosts: Hevea brasiliensis, H. guianensis, and Micandra sp. SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Skaltsas, D. N.] Univ Maryland, Silver Spring, MD USA. [Castlebury, L.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. [Chaverri, P.] Univ Maryland, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 134 EP 134 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500741 ER PT J AU Smith, BJ AF Smith, B. J. TI Fungicide applications affect fruit diseases and quality of muscadine grape (Vitis rotundifolia Michx.) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Smith, B. J.] USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 135 EP 136 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500747 ER PT J AU Smith, DL Groves, CL Fritz, C Willis, DK AF Smith, D. L. Groves, C. L. Fritz, C. Willis, D. K. TI Development and validation of quantitative polymerase chain reaction protocols for detection of Soybean vein necrosis virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Smith, D. L.; Groves, C. L.; Fritz, C.] Univ Wisconsin, Madison, WI USA. [Willis, D. K.] USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. [Willis, D. K.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. NR 0 TC 1 Z9 1 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 135 EP 135 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500746 ER PT J AU Stewart, CL Yuen, GY Vogel, K Pyle, JD Scholthof, KBG AF Stewart, C. L. Yuen, G. Y. Vogel, K. Pyle, J. D. Scholthof, K. B. G. TI Panicum mosaic virus-A potential threat to biofuel switchgrass production SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Stewart, C. L.; Yuen, G. Y.] Univ Nebraska, Lincoln, NE USA. [Vogel, K.] ARS, USDA, Lincoln, NE USA. [Pyle, J. D.; Scholthof, K. B. G.] Texas A&M Univ, College Stn, TX USA. NR 0 TC 1 Z9 1 U1 2 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 138 EP 138 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500760 ER PT J AU Stewart, JE Kroese, D Fieland, V Zasada, IA Grunwald, NJ AF Stewart, J. E. Kroese, D. Fieland, V. Zasada, I. A. Grunwald, N. J. TI Isolation and detection of Phytophthora rubi in raspberry (Rubus idaeus) production in the western United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Stewart, J. E.] Oregon State Univ, Corvallis, OR 97331 USA. [Kroese, D.; Zasada, I. A.] ARS, USDA, Corvallis, OR USA. [Fieland, V.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Grunwald, N. J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 139 EP 139 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500765 ER PT J AU Stobbe, A Schneider, W Melcher, U AF Stobbe, A. Schneider, W. Melcher, U. TI A bioinformatic pipeline for use in metagenomic virus discovery SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Stobbe, A.; Melcher, U.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W.] ARS, USDA, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 139 EP 139 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500766 ER PT J AU Strausbaugh, CA Wenninger, EJ Eujayl, IA AF Strausbaugh, C. A. Wenninger, E. J. Eujayl, I. A. TI Management of curly top in sugar beet with seed and foliar insecticides SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Strausbaugh, C. A.; Eujayl, I. A.] ARS, USDA, NWISRL, Kimberly, ID USA. [Wenninger, E. J.] Univ Idaho, Kimberly, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 139 EP 140 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500769 ER PT J AU Strauss, SL Kluepfel, D AF Strauss, S. L. Kluepfel, D. TI Impact of biological amendments on Agrobacterium tumefaciens soil survival SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Strauss, S. L.; Kluepfel, D.] ARS, USDA, Davis, CA USA. RI Strauss, Sarah/I-5141-2016 OI Strauss, Sarah/0000-0001-7494-5320 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 140 EP 140 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500770 ER PT J AU Stulberg, MJ Shao, J Huang, Q AF Stulberg, M. J. Shao, J. Huang, Q. TI Improvement of current detection and identification methods for select agent strains of Ralstonia solanacearum via multiplex PCR and qPCR SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Stulberg, M. J.; Shao, J.; Huang, Q.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 140 EP 140 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500771 ER PT J AU Sudarshana, MR Gonzalez, A Dave, A Wei, A Smith, R Anderson, MM Walker, AM AF Sudarshana, M. R. Gonzalez, A. Dave, A. Wei, A. Smith, R. Anderson, M. M. Walker, A. M. TI Grapevine red blotch-associated virus is widespread in California and US vineyards SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Sudarshana, M. R.; Gonzalez, A.; Dave, A.] ARS, USDA, Davis, CA USA. [Wei, A.] Agri Anal LLC, West Sacramento, CA USA. [Smith, R.; Anderson, M. M.; Walker, A. M.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 1 Z9 1 U1 1 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 140 EP 140 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500773 ER PT J AU Tatineni, S French, R AF Tatineni, S. French, R. TI Deletions in the coat protein cistron of Wheat streak mosaic virus induced more severe symptoms than the wild-type virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Tatineni, S.; French, R.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 143 EP 143 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500789 ER PT J AU Thies, J Levi, A AF Thies, J. Levi, A. TI Response of African horned cucumber (Cucumis metulifer) to southern root-knot nematode, Meloidogyne incognita SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Thies, J.] USDA ARS, Charleston, SC USA. [Levi, A.] USDA ARS, US Vegetable Lab, Charleston, SC USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 144 EP 144 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500795 ER PT J AU Thiessen, LD Mahaffee, W Keune, JA Grove, G AF Thiessen, L. D. Mahaffee, W. Keune, J. A. Grove, G. TI Real-time detection of airborne Erysiphe necator (grape powdery mildew) inoculum with loop-mediated isothermal amplification (LAMP) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Thiessen, L. D.; Keune, J. A.] Oregon State Univ, Corvallis, OR 97331 USA. [Mahaffee, W.] USDA ARS, Corvallis, OR USA. [Grove, G.] Washington State Univ, Prosser, WA USA. NR 0 TC 1 Z9 1 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 144 EP 144 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500796 ER PT J AU Travadon, R Rolshausen, P Gubler, WD Urbez-Torres, JR Baumgartner, K AF Travadon, R. Rolshausen, P. Gubler, W. D. Urbez-Torres, J. R. Baumgartner, K. TI High genetic diversity in North American populations of Phaeomoniella chlamydospora, causal agent of Petri disease and esca of grapevine SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Travadon, R.; Gubler, W. D.] Univ Calif Davis, Davis, CA 95616 USA. [Rolshausen, P.] Univ Calif Riverside, Riverside, CA 92521 USA. [Urbez-Torres, J. R.] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Summerland, BC, Canada. [Baumgartner, K.] USDA ARS, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 148 EP 148 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500814 ER PT J AU Vu, AL Daughtrey, ML Crouch, JA Wick, RL AF Vu, A. L. Daughtrey, M. L. Crouch, J. A. Wick, R. L. TI Comparison of Peronospora species causing downy mildew on basil, coleus, and agastache SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Vu, A. L.; Wick, R. L.] Univ Massachusetts, Amherst, MA 01003 USA. [Daughtrey, M. L.] Cornell Univ, LIHREC, Riverhead, NY USA. [Crouch, J. A.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 154 EP 154 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500848 ER PT J AU Wallingford, AK Wallis, CM Chen, J AF Wallingford, A. K. Wallis, C. M. Chen, J. TI Effects of rootstock on Xylella fastidiosa infection and grapevine sap phenolics SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wallingford, A. K.; Wallis, C. M.; Chen, J.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 154 EP 154 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500849 ER PT J AU Wallis, CM Rashed, A Wallingford, AK Rush, CM AF Wallis, C. M. Rashed, A. Wallingford, A. K. Rush, C. M. TI Relationship of potato biochemical responses to 'Candidatus Liberibacter solanacearum', causal agent of zebra chip, to disease progression SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wallis, C. M.; Wallingford, A. K.] ARS, USDA, Parlier, CA USA. [Rashed, A.; Rush, C. M.] Texas AgriLife Res, Amarillo, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 154 EP 154 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500850 ER PT J AU Wallis, CM Wallingford, AK AF Wallis, C. M. Wallingford, A. K. TI Effects of grapevine sap phenolics on the in vitro growth of Xylella fastidiosa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wallis, C. M.; Wallingford, A. K.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 154 EP 155 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500851 ER PT J AU Wan, A Chen, X AF Wan, A. Chen, X. TI Stripe rust epidemics of wheat and barley and races of Puccinia striiformis identified in the United States in 2012 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wan, A.] Washington State Univ, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 155 EP 155 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500853 ER PT J AU Wang, H McTavish, C Turechek, W AF Wang, H. McTavish, C. Turechek, W. TI Monitoring the infection process of Xanthomonas fragariae in strawberry with a GFP-labeled strain SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wang, H.] Univ Florida, USDA ARS, Ft Pierce, FL USA. [McTavish, C.] Michigan State Univ, E Lansing, MI 48824 USA. [Turechek, W.] USDA ARS, Ft Pierce, FL USA. NR 0 TC 1 Z9 1 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 155 EP 155 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500855 ER PT J AU Wang, M Chen, X AF Wang, M. Chen, X. TI Association mapping for stripe rust resistance genes in spring wheat germplasm lines SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wang, M.] Washington State Univ, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 155 EP 155 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500854 ER PT J AU Wang, J Yokomi, R Lee, R Folimonova, SY Vidalakis, G AF Wang, J. Yokomi, R. Lee, R. Folimonova, S. Y. Vidalakis, G. TI Molecular diversity of Citrus tristeza virus in California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wang, J.; Vidalakis, G.] Univ Calif Riverside, Riverside, CA 92521 USA. [Yokomi, R.] USDA ARS, Parlier, CA USA. [Lee, R.] USDA ARS, Riverside, CA USA. [Folimonova, S. Y.] Univ Florida, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500858 ER PT J AU Wang, M Xia, C Wan, A Chen, X AF Wang, M. Xia, C. Wan, A. Chen, X. TI Association of single nucleotide polymorphism markers based on secreted protein genes of Puccinia striiformis f. sp tritici to avirulence genes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wang, M.; Xia, C.; Wan, A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Dept Plant Pathol, Pullman, WA 99164 USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 156 EP 157 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500862 ER PT J AU Wang, M Postman, J Li, R AF Wang, M. Postman, J. Li, R. TI Development of loop mediated isothermal amplification reaction and TaqMan real-time PCR for the detection of Gooseberry vein banding associated virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wang, M.; Li, R.] USDA ARS, Beltsville, MD USA. [Postman, J.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500859 ER PT J AU Wang, X Mavrodi, DV Ke, L Mavrodi, OV Yang, M Zheng, N Tomashow, L Weller, DM Zhang, J AF Wang, X. Mavrodi, D. V. Ke, L. Mavrodi, O. V. Yang, M. Zheng, N. Tomashow, L. Weller, D. M. Zhang, J. TI Biocontrol and growth-promoting activity of rhizobacteria from Chinese contaminated soils SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wang, X.; Ke, L.; Zheng, N.; Zhang, J.] Huazhong Agr Univ, Wuhan, Peoples R China. [Mavrodi, D. V.; Mavrodi, O. V.; Yang, M.] Washington State Univ, Pullman, WA 99164 USA. [Tomashow, L.; Weller, D. M.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500861 ER PT J AU Wang, X Yokomi, R Chen, J AF Wang, X. Yokomi, R. Chen, J. TI Sensitive detection of Spiroplasma citri by targeting prophage sequences SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wang, X.] South West Univ, Chongqing, Peoples R China. [Yokomi, R.] USDA ARS, Parlier, CA USA. [Chen, J.] USDA ARS PWA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 157 EP 157 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500865 ER PT J AU Washington-Keizerweerd, AT Warnke, KZ Grisham, MP AF Washington-Keizerweerd, A. T. Warnke, K. Z. Grisham, M. P. TI Genetic diversity of viruses causing mosaic in Louisiana sugarcane SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Washington-Keizerweerd, A. T.; Warnke, K. Z.; Grisham, M. P.] USDA ARS, Sugarcane Res Unit, Houma, LA 70361 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 158 EP 158 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500871 ER PT J AU Weaver, M Abbas, H Sciumbato, G Pringle, HC Allen, T AF Weaver, M. Abbas, H. Sciumbato, G. Pringle, H. C. Allen, T. TI Aflatoxin management in corn with Afla-Guard SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Weaver, M.; Abbas, H.] USDA ARS, Stoneville, MS 38776 USA. [Sciumbato, G.; Pringle, H. C.; Allen, T.] Mississippi State Univ MAFES, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 158 EP 158 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500872 ER PT J AU Webster, CG Frantz, G Mellinger, HC McAvoy, E Funderburk, J Adkins, S AF Webster, C. G. Frantz, G. Mellinger, H. C. McAvoy, E. Funderburk, J. Adkins, S. TI Emergence and impact of two tospoviruses in Florida SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Webster, C. G.; Adkins, S.] USDA ARS USHRL, Ft Pierce, FL USA. [Frantz, G.; Mellinger, H. C.] Glades Crop Care Inc, Jupiter, FL USA. [McAvoy, E.] Univ Florida IFAS Extens, Labelle, FL USA. [Funderburk, J.] Univ Florida, Quincy, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 158 EP 159 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500873 ER PT J AU Weiland, J Garrido, P Grunwald, NJ Garzon, C AF Weiland, J. Garrido, P. Grunwald, N. J. Garzon, C. TI Exploring the characteristics of Pythium communities: Can knowledge about pathogen communities improve disease control? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Weiland, J.; Grunwald, N. J.] USDA ARS, Corvallis, OR USA. [Garrido, P.; Garzon, C.] Oklahoma State Univ, Stillwater, OK 74078 USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 159 EP 159 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500875 ER PT J AU Widmer, T AF Widmer, T. TI Use of a fungal "cocktail" to inhibit growth of Phytophthora cinnamomi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Widmer, T.] USDA ARS FDWSRU, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 160 EP 160 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500879 ER PT J AU Wintermantel, WM AF Wintermantel, W. M. TI Genome analysis and biological characterization of Moroccan pepper virus (MPV), and reclassification of Lettuce necrotic stunt virus as MPV SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wintermantel, W. M.] USDA ARS, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 160 EP 160 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500883 ER PT J AU Wintermantel, WM Hladky, LL AF Wintermantel, W. M. Hladky, L. L. TI Genome characterization of Tomato necrotic dwarf virus, a Torradovirus from southern California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Wintermantel, W. M.; Hladky, L. L.] USDA ARS, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 160 EP 160 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500882 ER PT J AU Wiseman, MS Dugan, FM Xiao, CL AF Wiseman, M. S. Dugan, F. M. Xiao, C. L. TI A postharvest fruit rot of apple caused by Lambertella sp in Washington State SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wiseman, M. S.] Washington State Univ, Pullman, WA 99164 USA. [Dugan, F. M.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Xiao, C. L.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 160 EP 161 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500884 ER PT J AU Wolfenbarger, S Gadoury, D Twomey, M Welser, MJ Gent, D AF Wolfenbarger, S. Gadoury, D. Twomey, M. Welser, M. J. Gent, D. TI Mating type distribution and the absence of cleistothecia of Podosphaera macularis in the Pacific Northwest SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Wolfenbarger, S.; Twomey, M.; Gent, D.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gadoury, D.; Welser, M. J.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY USA. [Gent, D.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 161 EP 161 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500885 ER PT J AU Workneh, F Henne, DC Goolsby, JA Crosslin, JM Whipple, SD Bradshaw, JD Rashed, A Paetzold, L Harveson, RM Rush, CM AF Workneh, F. Henne, D. C. Goolsby, J. A. Crosslin, J. M. Whipple, S. D. Bradshaw, J. D. Rashed, A. Paetzold, L. Harveson, R. M. Rush, C. M. TI Assessments of potential management and environmental factors affecting regional occurrence of potato zebra chip disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Workneh, F.; Rashed, A.; Paetzold, L.; Rush, C. M.] Texas A&M AgriLife Res, Bushland, TX USA. [Henne, D. C.] Texas A&M AgriLife Res, Weslaco, TX USA. [Goolsby, J. A.] USDA ARS, Edinburg, TX USA. [Crosslin, J. M.] USDA ARS, Prosser, WA 99350 USA. [Whipple, S. D.; Bradshaw, J. D.; Harveson, R. M.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE USA. NR 0 TC 0 Z9 0 U1 2 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 162 EP 162 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500890 ER PT J AU Xiao, CL Boal, RJ AF Xiao, C. L. Boal, R. J. TI Sources and availability of Sphaeropsis pyriputrescens inoculum in apple orchards SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Xiao, C. L.] USDA ARS, Parlier, CA USA. [Boal, R. J.] Washington State Univ, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 163 EP 163 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500898 ER PT J AU Yaghmour, MA Nguyen, TL Roubtsova, TV Hasey, J DeBuse, C Fichtner, EJ Hoenisch, R Seybold, S Bostock, RM AF Yaghmour, M. A. Nguyen, T. L. Roubtsova, T. V. Hasey, J. DeBuse, C. Fichtner, E. J. Hoenisch, R. Seybold, S. Bostock, R. M. TI Thousand cankers disease in California English walnut: Incidence, distribution, and characterization of Geosmithia morbida SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Yaghmour, M. A.; Nguyen, T. L.; Roubtsova, T. V.; DeBuse, C.; Hoenisch, R.; Bostock, R. M.] Univ Calif Davis, Davis, CA 95616 USA. [Hasey, J.] Univ Calif Cooperat Extens, Yuba, CA USA. [Fichtner, E. J.] Univ Calif Cooperat Extens, Tulare, CA USA. [Bostock, R. M.] US Forest Serv, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 2 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 163 EP 163 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500900 ER PT J AU Yang, HC Haudenshield, JS Hartman, GL AF Yang, H. C. Haudenshield, J. S. Hartman, G. L. TI Real-time PCR detection and differentiation of four Colletotrichum species causing soybean anthracnose SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Yang, H. C.] Univ Illinois, Urbana, IL 61801 USA. [Haudenshield, J. S.] USDA ARS, Urbana, IL USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 164 EP 164 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500901 ER PT J AU Yang, X Copes, WE AF Yang, X. Copes, W. E. TI Three novel Phytophthora species from irrigation water in Mississippi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Yang, X.] Virginia Tech, Virginia Beach, VA USA. [Copes, W. E.] USDA ARS, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 164 EP 164 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500903 ER PT J AU Yasmin, T Hobbs, HA Domier, LL Nelson, BD AF Yasmin, T. Hobbs, H. A. Domier, L. L. Nelson, B. D. TI Molecular characterization of a novel soybean-infecting nepovirus from North Dakota SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Yasmin, T.; Hobbs, H. A.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Domier, L. L.] USDA ARS, Urbana, IL USA. [Nelson, B. D.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 164 EP 164 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500904 ER PT J AU Yuan, Q Ding, F Minenkova, O Brlansky, R Jordan, R Hartung, J AF Yuan, Q. Ding, F. Minenkova, O. Brlansky, R. Jordan, R. Hartung, J. TI Development and application of scFv for 'Ca. Liberibacter asiaticus', the pathogen associated with huanglongbing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Yuan, Q.] Luzhou Med Coll, Luzhou, Peoples R China. [Ding, F.; Jordan, R.] USDA ARS, Beltsville, MD USA. [Minenkova, O.] Sigma Tau Pharmaceut, Rome, Italy. [Brlansky, R.] Univ Florida, Lake Alfred, FL USA. [Hartung, J.] USDA ARS MPPL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 165 EP 165 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500910 ER PT J AU Zhang, Z Shao, Z Tang, J Jin, X Thomashow, LS Weller, DM Okubara, P Zhang, J AF Zhang, Z. Shao, Z. Tang, J. Jin, X. Thomashow, L. S. Weller, D. M. Okubara, P. Zhang, J. TI Biocontrol efficacy and mechanism of action against root-knot nematode of Pseudomonas putida A316 from South Pole soil SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Zhang, Z.; Tang, J.; Jin, X.; Zhang, J.] Huazhong Agr Univ, Wuhan, Peoples R China. [Shao, Z.] Third Inst Oceanog State Ocean Adm, Xiamen, Peoples R China. [Thomashow, L. S.; Weller, D. M.; Okubara, P.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. RI Tang, Juming/A-4027-2009 OI Tang, Juming/0000-0001-9449-1004 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 166 EP 167 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500916 ER PT J AU Zhao, L Levy, L Di, R AF Zhao, L. Levy, L. Di, R. TI Detection of Ralstonia solanacearum using portable surface plasmon resonance technology SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Zhao, L.; Di, R.] Rutgers State Univ, New Brunswick, NJ 08903 USA. [Levy, L.] USDA APHIS PPQ CPHST, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 167 EP 168 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500921 ER PT J AU Zhou, XG Liu, G Anders, MM Jia, Y Allen, TW Lu, S Reddy, MS Kloepper, JW AF Zhou, X. G. Liu, G. Anders, M. M. Jia, Y. Allen, T. W. Lu, S. Reddy, M. S. Kloepper, J. W. TI Multistate evaluation of PGPR strain MBI600 and its combined use with azoxystrobin for control of sheath blight in rice SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Zhou, X. G.; Liu, G.] Texas A&M AgriLife Res, Beaumont, TX USA. [Anders, M. M.] Univ Arkansas, Stuttgart, AR USA. [Jia, Y.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Allen, T. W.] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS USA. [Lu, S.] Mississippi State Univ, Mississippi State, MS 39762 USA. [Reddy, M. S.; Kloepper, J. W.] Auburn Univ, Auburn, AL 36849 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 168 EP 168 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500924 ER PT J AU Zhou, XG McClung, AM AF Zhou, X. G. McClung, A. M. TI Efficacy of Bacillus biocontrol agents for management of sheath blight and narrow brown leaf spot in organic rice SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Zhou, X. G.] Texas A&M AgriLife Res, Beaumont, TX USA. [McClung, A. M.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 168 EP 168 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500922 ER PT J AU Zhu, Y Shin, S Johnson, F Mazzola, M AF Zhu, Y. Shin, S. Johnson, F. Mazzola, M. TI The activation of ethylene and jasmonic acid pathways in apple rootstock during Pythium ultimum infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Zhu, Y.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA USA. [Shin, S.; Mazzola, M.] ARS, USDA, Wenatchee, WA USA. [Johnson, F.] Washington State Univ, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 169 EP 169 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500927 ER PT J AU Redinbaugh, M Correa, V Majerczak, DR Ammar, ED Merighi, M Pratt, R Hogenhout, S Coplin, D AF Redinbaugh, M. Correa, V. Majerczak, D. R. Ammar, E. D. Merighi, M. Pratt, R. Hogenhout, S. Coplin, D. TI Pantoea stewartii uses distinct type III secretion systems to alternate between host kingdoms SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Redinbaugh, M.] ARS, USDA, Wooster, OH USA. [Redinbaugh, M.] Ohio State Univ, OARDC, Wooster, OH USA. [Correa, V.; Majerczak, D. R.] Ohio State Univ, Wooster, OH USA. [Ammar, E. D.] ARS, USDA, Ft Pierce, FL USA. [Merighi, M.; Coplin, D.] Ohio State Univ, Columbus, OH 43210 USA. [Pratt, R.] New Mexico State Univ, Las Cruces, NM 88003 USA. [Hogenhout, S.] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 171 EP 171 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501007 ER PT J AU Geiser, DM O'Donnell, K AF Geiser, D. M. O'Donnell, K. TI Defining the genus Fusarium in a scientifically robust way that best preserves longstanding use SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Geiser, D. M.] Penn State Univ, University Pk, PA 16802 USA. [O'Donnell, K.] ARS, USDA, NCAUR Bacterial Foodborne Pathogens & Mycol Unit, Peoria, IL USA. RI Geiser, David/J-9950-2013 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 172 EP 172 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501013 ER PT J AU Palm, ME Rossman, AY AF Palm, M. E. Rossman, A. Y. TI Impact of ICN changes on scientific names of regulated fungal plant pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Palm, M. E.] USDA APHIS PPQ, Riverdale, MD USA. [Rossman, A. Y.] USDA ARS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 172 EP 172 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501011 ER PT J AU Cotty, PJ Ortega-Beltran, A Jaime, R AF Cotty, P. J. Ortega-Beltran, A. Jaime, R. TI Influences of weather on aflatoxin-producing fungi and aflatoxin concentrations in crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cotty, P. J.] ARS, USDA, Tucson, AZ USA. [Ortega-Beltran, A.; Jaime, R.] Univ Arizona, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 175 EP 175 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501026 ER PT J AU Doster, MA Cotty, PJ Puckett, RD Morgan, DP Michailides, TJ AF Doster, M. A. Cotty, P. J. Puckett, R. D. Morgan, D. P. Michailides, T. J. TI Challenges in using the biopesticide AF36 in pistachio orchards to reduce aflatoxin contamination SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Doster, M. A.; Puckett, R. D.; Morgan, D. P.; Michailides, T. J.] Univ Calif, Parlier, CA USA. [Cotty, P. J.] Univ Arizona, USDA ARS, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 175 EP 175 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501027 ER PT J AU Palumbo, JD O'Keeffe, TL AF Palumbo, J. D. O'Keeffe, T. L. TI When mycotoxins come in bunches: Fumonisin production by Aspergillus niger in grapes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Palumbo, J. D.; O'Keeffe, T. L.] USDA ARS WRRC, Albany, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 175 EP 176 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501029 ER PT J AU Abbas, HK Weaver, MA Shier, WT Zablotowicz, RM Plasencia, JD AF Abbas, H. K. Weaver, M. A. Shier, W. T. Zablotowicz, R. M. Plasencia, J. D. TI Aflatoxin and fumonisin contamination in corn smut (Ustilago maydis) galls in the field and in the grocery store SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Abbas, H. K.; Weaver, M. A.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Shier, W. T.] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA. [Zablotowicz, R. M.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Plasencia, J. D.] Univ Mexico, Mexico City, DF, Mexico. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 176 EP 176 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501030 ER PT J AU Schneider, W Verma, R Stobbe, A Daniels, J Espindola, A Blagden, T Fletcher, J Ochoa-Corona, FM Garzon, C Melcher, U AF Schneider, W. Verma, R. Stobbe, A. Daniels, J. Espindola, A. Blagden, T. Fletcher, J. Ochoa-Corona, F. M. Garzon, C. Melcher, U. TI Bioinformatics strategies for microbial forensics SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Schneider, W.] USDA ARS, Ft Detrick, MD USA. [Verma, R.; Stobbe, A.; Daniels, J.; Espindola, A.; Blagden, T.; Fletcher, J.; Ochoa-Corona, F. M.; Garzon, C.; Melcher, U.] Oklahoma State Univ, Natl Inst Microbial Forens & Food & Agr Biosecur, Stillwater, OK 74078 USA. RI Espindola, Andres/A-5056-2016 OI Espindola, Andres/0000-0002-9658-0673 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 176 EP 176 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501033 ER PT J AU Lee, RF AF Lee, R. F. TI Cadang-cadang disease of palm and other diseases SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lee, R. F.] USDA ARS, Riverside, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 177 EP 177 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501036 ER PT J AU Peterson, GL AF Peterson, G. L. TI Biology of downy mildews from gramineaceous crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Peterson, G. L.] USDA ARS, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 178 EP 178 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501043 ER PT J AU Abad, ZG Bienapfl, J Luster, DG Carter, M Thines, M Telle, S Riley, MJJ Levesque, A Shivas, RG Dela Cueva, FM Crouch, JA Nakhla, MK AF Abad, Z. G. Bienapfl, J. Luster, D. G. Carter, M. Thines, M. Telle, S. Riley, M. J. J. Levesque, A. Shivas, R. G. Dela Cueva, F. M. Crouch, J. A. Nakhla, M. K. TI Status, challenges and tools for identification and diagnosis of Peronosclerospora and Sclerophthora of regulatory concern for graminicolous crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Abad, Z. G.; Bienapfl, J.; Nakhla, M. K.] USDA APHIS PPQ CPHST Beltsville Lab, Beltsville, MD USA. [Luster, D. G.; Carter, M.] USDA ARS, Frederick, MD USA. [Thines, M.] Goethe Univ Frankfurt, Dept Biol Sci, Inst Ecol Evol, D-60054 Frankfurt, Germany. [Thines, M.] Goethe Univ Frankfurt, D-60054 Frankfurt, Germany. [Riley, M. J. J.; Shivas, R. G.] Agri Sci Queensland, Plant Pathol Herbarium, Brisbane, Qld, Australia. [Levesque, A.] Agr & Agri Food Canada, Ottawa, ON, Canada. [Dela Cueva, F. M.] Univ Philippines, Laguna, Philippines. [Crouch, J. A.] USDA ARS, Systemat Mycol & Microbiol Labs, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 179 EP 179 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501047 ER PT J AU Cullen, D Floudas, D Hibbett, D AF Cullen, D. Floudas, D. Hibbett, D. TI Mechanisms of wood decay inferred from recent genome investigations SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cullen, D.] USDA, Forest Prod Lab, Madison, WI 53705 USA. [Floudas, D.; Hibbett, D.] Clark Univ, Worcester, MA 01610 USA. NR 0 TC 0 Z9 0 U1 0 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 180 EP 180 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501053 ER PT J AU Gutierrez, W AF Gutierrez, W. TI Free trade, fair trade, safe trade: The role of plant pathology in filling regulatory gaps SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Gutierrez, W.] USDA, APHIS, PPQ, Ctr Plant Hlth Sci & Technol, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 180 EP 180 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501052 ER PT J AU Cox, F Barsoum, N Lilleskov, E Bidartondo, M AF Cox, F. Barsoum, N. Lilleskov, E. Bidartondo, M. TI Does nitrogen availability affect ectomycorrhizal fungal communities at the regional scale? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cox, F.] Univ Manchester, Manchester, Lancs, England. [Barsoum, N.] Alice Holt, Forest Res, Farnham, Surrey, England. [Lilleskov, E.] US Forest Serv, USDA, Houghton, MI USA. [Bidartondo, M.] Royal Bot Gardens Kew, London, England. [Bidartondo, M.] Univ London Imperial Coll Sci Technol & Med, London, England. NR 0 TC 0 Z9 0 U1 1 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 181 EP 181 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501062 ER PT J AU Taylor, L Hollingsworth, T McFarland, J Ruess, R Timling, I Walker, D AF Taylor, L. Hollingsworth, T. McFarland, J. Ruess, R. Timling, I. Walker, D. TI Aboveground-belowground linkages: Extrapolating local to global fungal biodiversity SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Taylor, L.] Univ New Mexico, Albuquerque, NM 87131 USA. [Hollingsworth, T.] US Forest Serv, USDA, PNW Res Stn, Boreal Ecol Cooperat Res Unit, Fairbanks, AK USA. [McFarland, J.] US Geol Survey, Menlo Pk, CA 94025 USA. [Ruess, R.; Timling, I.; Walker, D.] Univ Alaska, Fairbanks, AK 99701 USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 182 EP 182 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501064 ER PT J AU Guo, B Liu, X Ozias-Akins, P Zhang, X Liao, B Varshney, RK Nwosu, V Wilson, RF Stalker, HT AF Guo, B. Liu, X. Ozias-Akins, P. Zhang, X. Liao, B. Varshney, R. K. Nwosu, V. Wilson, R. F. Stalker, H. T. TI The Peanut Genome Consortium and Peanut Genome Sequence: Creating a better future through global food security SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Guo, B.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Liu, X.] BGI ShenZhen, Shenzhen, Peoples R China. [Ozias-Akins, P.] Univ Georgia, Tifton, GA USA. [Zhang, X.] Henan Acad Agr Sci, Zhengzhou, Peoples R China. [Liao, B.] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan, Peoples R China. [Varshney, R. K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Nwosu, V.] Mars Chocolate North Amer, Plant Sci Program, Global Chocolate Sci & Technol, Hackettstown, NJ USA. [Wilson, R. F.] Oilseeds & Biosci Consulting, Raleigh, NC USA. [Stalker, H. T.] N Carolina State Univ, Raleigh, NC 27695 USA. RI Varshney, Rajeev/C-5295-2014 OI Varshney, Rajeev/0000-0002-4562-9131 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 183 EP 184 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501073 ER PT J AU Chen, X AF Chen, X. TI Population structure and genomics of the stripe rust pathogen and interactions with its host plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 184 EP 184 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501074 ER PT J AU Jia, Y AF Jia, Y. TI Global efforts in managing rice blast disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Jia, Y.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 184 EP 184 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501075 ER PT J AU Hammond, J Vaira, AM Lim, HS AF Hammond, J. Vaira, A. M. Lim, H. S. TI The importance of chloroplast interactions for local and systemic movement of some members of the Alphaflexiviridae SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Hammond, J.] USDA ARS, USNA, FNPRU, Beltsville, MD USA. [Vaira, A. M.] CNR, IVV, I-10126 Turin, Italy. [Lim, H. S.] Chungnam Natl Univ, Taejon, South Korea. RI Vaira, Anna Maria/B-9082-2015 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 188 EP 188 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799501100 ER PT J AU Kyamuhangire, W Lubowa, A Kaaya, A Kikafunda, J Harvey, PWJ Rambeloson, Z Dary, O Dror, DK Allen, LH AF Kyamuhangire, William Lubowa, Abdelrahman Kaaya, Archileo Kikafunda, Joyce Harvey, Philip W. J. Rambeloson, Zo Dary, Omar Dror, Daphna K. Allen, Lindsay H. TI The importance of using food and nutrient intake data to identify appropriate vehicles and estimate potential benefits of food fortification in Uganda SO FOOD AND NUTRITION BULLETIN LA English DT Article DE Calcium; folate; folic acid; food fortification; iron; maize flour fortification; micronutrient inadequacy; niacin; oil fortification; riboflavin; sugar fortification; thiamine; Uganda; vitamin A; vitamin B-6; vitamin B-12; vitamin C; wheat flour fortification; zinc ID INTAKE DISTRIBUTIONS AB Background. Concern over micronutrient inadequacies in Uganda has prompted the introduction of mass fortification. Objective. To use food intake to determine nutrient inadequacies in children aged 24 to 59 months and nonpregnant women of reproductive age, and to model the adequacy of mass fortification. Methods. Data were collected by the 24-hour recall method in three regions. Usual nutrient intakes were calculated by adjusting actual intake distribution for the intraindividual variance. The impact of fortification on intake adequacy was simulated. Results. The nutrients with the highest prevalence of inadequate intake across regions were vitamin A (30% to 99%), vitamin B-12 (32% to 100%), iron (55% to 89%), zinc (18% to 82%), and calcium (84% to 100%). According to simulations, fortification of vegetable oil and sugar with vitamin A would reduce the prevalence of vitamin A inadequacy in the Western and Northern regions; in Kampala it would eliminate vitamin A inadequacy but would cause 2% to 48% of children to exceed the Tolerable Upper Intake Level (UL). The proposed fortification of wheat flour would reduce the prevalence of inadequate intakes of thiamine, riboflavin, folate, and niacin in Kampala, but would have little impact in the other two regions due to low flour consumption. Conclusions. Micronutrient fortification of vegetable oil and sugar in all regions and of wheat flour in Kampala would reduce the prevalence of rnicronutrient inadequacies. However, the wheat flour formulation should be modified to better meet requirements, and the vitamin A content in sugar should be reduced to minimize the risk of high intakes. Maize flour may be suitable for targeted fortification, but prior consolidation of the industry would be required for maize flour to become a good vehicle for mass fortification. C1 [Kyamuhangire, William; Lubowa, Abdelrahman; Kaaya, Archileo; Kikafunda, Joyce] Makerere Univ, Sch Food Technol Nutr & Bioengn, Kampala, Uganda. [Rambeloson, Zo] FHI 360, Washington, DC USA. [Dary, Omar] Abt Assoc, Bethesda, MD 20814 USA. [Dror, Daphna K.; Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. RP Dary, O (reprint author), Abt Assoc, 4550 Montgomery Ave,Suite 800 N, Bethesda, MD 20814 USA. FU American people through the United States Agency for International Development (USAID) [GHS-A-00-05-00012-00]; USAID; Global Alliance for Improved Nutrition (GAIN); World Food Programme FX This article was made possible by the generous support of the American people through the United States Agency for International Development (USAID) under the terms of Cooperative Agreement No. GHS-A-00-05-00012-00 to FHI-360 (and previously to the Academy for Educational Development). The database used in this study came from the 2008 Ugandan Food Consumption Survey cofinanced by USAID, the Global Alliance for Improved Nutrition (GAIN), and the World Food Programme. The contents are the responsibility of the authors and do not necessarily reflect the views of USAID, the United States Government, GAIN, or the World Food Programme. NR 17 TC 6 Z9 6 U1 0 U2 12 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2013 VL 34 IS 2 BP 131 EP 142 PG 12 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 189RJ UT WOS:000322285700002 PM 23964386 ER PT J AU Allen, L AF Allen, Lindsay TI Comparing the value of protein sources for maternal and child nutrition SO FOOD AND NUTRITION BULLETIN LA English DT Article ID MILK CONSUMPTION; GROWTH; PREGNANCY; WEIGHT; BIRTH; DIET; MEAT C1 Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Allen, L (reprint author), Univ Calif Davis, USDA ARS, Western Human Nutr Res Ctr, 431 W Hlth Sci Dr, Davis, CA 95616 USA. EM lindsay.allen@ars.usda.gov NR 12 TC 4 Z9 4 U1 0 U2 7 PU INT NUTRITION FOUNDATION PI BOSTON PA 150 HARRISON AVE, BOSTON, MA 02111 USA SN 0379-5721 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD JUN PY 2013 VL 34 IS 2 BP 263 EP 266 PG 4 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 189RJ UT WOS:000322285700023 PM 23964407 ER PT J AU Meihls, LN Handrick, V Glauser, G Barbier, H Kaur, H Haribal, MM Lipka, AE Gershenzon, J Buckler, ES Erb, M Kollner, TG Jander, G AF Meihls, Lisa N. Handrick, Vinzenz Glauser, Gaetan Barbier, Hugues Kaur, Harleen Haribal, Meena M. Lipka, Alexander E. Gershenzon, Jonathan Buckler, Edward S. Erb, Matthias Koellner, Tobias G. Jander, Georg TI Natural Variation in Maize Aphid Resistance Is Associated with 2,4-Dihydroxy-7-Methoxy-1,4-Benzoxazin-3-One Glucoside Methyltransferase Activity SO PLANT CELL LA English DT Article ID QUANTITATIVE TRAIT LOCUS; RIBOSOME-INACTIVATING PROTEIN; INNATE IMMUNITY; MAPPING POPULATION; MOLECULAR ANALYSIS; INSECT RESISTANCE; TRITICUM-AESTIVUM; HYDROXAMIC ACIDS; PLANT DEFENSES; FALL ARMYWORM AB Plants differ greatly in their susceptibility to insect herbivory, suggesting both local adaptation and resistance tradeoffs. We used maize (Zea mays) recombinant inbred lines to map a quantitative trait locus (QTL) for the maize leaf aphid (Rhopalosiphum maidis) susceptibility to maize Chromosome 1. Phytochemical analysis revealed that the same locus was also associated with high levels of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (HDMBOA-Glc) and low levels of 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside (DIMBOA-Glc). In vitro enzyme assays with candidate genes from the region of the QTL identified three O-methyltransferases (Bx10a-c) that convert DIMBOA-Glc to HDMBOA-Glc. Variation in HDMBOA-Glc production was attributed to a natural CACTA family transposon insertion that inactivates Bx10c in maize lines with low HDMBOA-Glc accumulation. When tested with a population of 26 diverse maize inbred lines, R. maidis produced more progeny on those with high HDMBOA-Glc and low DIMBOA-Glc. Although HDMBOA-Glc was more toxic to R. maidis than DIMBOA-Glc in vitro, BX10c activity and the resulting decline of DIMBOA-Glc upon methylation to HDMBOA-Glc were associated with reduced callose deposition as an aphid defense response in vivo. Thus, a natural transposon insertion appears to mediate an ecologically relevant trade-off between the direct toxicity and defense-inducing properties of maize benzoxazinoids. C1 [Meihls, Lisa N.; Barbier, Hugues; Kaur, Harleen; Haribal, Meena M.; Jander, Georg] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Handrick, Vinzenz; Gershenzon, Jonathan; Erb, Matthias; Koellner, Tobias G.] Max Planck Inst Chem Ecol, D-07745 Jena, Germany. [Glauser, Gaetan] Univ Neuchatel, Inst Biol, CH-2009 Neuchatel, Switzerland. [Lipka, Alexander E.; Buckler, Edward S.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Jander, G (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM koellner@ice.mpg.de; jander@cornell.edu RI Gershenzon, Jonathan/K-1331-2013; Kollner, Tobias/H-3375-2014; OI Erb, Matthias/0000-0002-4446-9834; Gershenzon, Jonathan/0000-0002-1812-1551; Kollner, Tobias/0000-0002-7037-904X; Buckler, Edward/0000-0002-3100-371X FU US National Science Foundation [IOS-1139329, DBI-0820619]; Defense Advanced Research Projects Agency Award [W31P4Q-10-1-0011]; Friedrich Wilhelm Bessel Research Award from the Alexander von Humboldt Foundation; USDA [2011-67012-30675]; USDA-Agricultural Research Service; Sinergia Grant of the Swiss National Science Foundation [SNF 136184]; Marie Curie Intra European Fellowship [273107]; Microsoft Corporation FX This research was funded by US National Science Foundation Award IOS-1139329, Defense Advanced Research Projects Agency Award W31P4Q-10-1-0011, and a Friedrich Wilhelm Bessel Research Award from the Alexander von Humboldt Foundation to G.J.; USDA Award 2011-67012-30675 to L.N.M.; and the USDA-Agricultural Research Service and US National Science Foundation Award DBI-0820619 to E.S.B. Research activities of J.G., V.H., T.G.K., G.G., and M.E. were supported by a Sinergia Grant of the Swiss National Science Foundation (SNF 136184). The work of M.E. is supported by a Marie Curie Intra European Fellowship (Grant 273107). We thank Gabriella Gomez for assistance with callose assays, Neil Villard for benzoxazinoid measurements, and I-Chun Chen for aphid bioassays. Part of this work was carried out using the resources of the Computational Biology Service Unit from Cornell University, which is partially funded by Microsoft Corporation. NR 70 TC 44 Z9 45 U1 11 U2 90 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD JUN PY 2013 VL 25 IS 6 BP 2341 EP 2355 DI 10.1105/tpc.113.112409 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 190VV UT WOS:000322371500033 PM 23898034 ER PT J AU Rossman, A Udayanga, D Castlebury, LA Hyde, KD AF Rossman, Amy Udayanga, Dhanushka Castlebury, Lisa A. Hyde, Kevin D. TI (2141) Proposal to conserve the name Phomopsis citri HS Fawc. (Diaporthe citri), with a conserved type, against Phomopsis citri (Sacc.) Traverso & Spessa (Ascomycota: Diaporthales: Diaporthaceae) SO TAXON LA English DT Editorial Material C1 [Rossman, Amy; Udayanga, Dhanushka; Castlebury, Lisa A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. [Udayanga, Dhanushka; Hyde, Kevin D.] Mae Fah Luang Univ, Sch Sci, Inst Excellence Fungal Res, Chiang Rai 57100, Thailand. RP Rossman, A (reprint author), ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. EM Amy.Rossman@ars.usda.gov NR 11 TC 1 Z9 1 U1 1 U2 3 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 J9 TAXON JI Taxon PD JUN PY 2013 VL 62 IS 3 BP 627 EP 627 PG 1 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 196CN UT WOS:000322751100018 ER PT J AU Ward, AS Payn, RA Gooseff, MN McGlynn, BL Bencala, KE Kelleher, CA Wondzell, SM Wagener, T AF Ward, Adam S. Payn, Robert A. Gooseff, Michael N. McGlynn, Brian L. Bencala, Kenneth E. Kelleher, Christa A. Wondzell, Steven M. Wagener, Thorsten TI Variations in surface water-ground water interactions along a headwater mountain stream: Comparisons between transient storage and water balance analyses SO WATER RESOURCES RESEARCH LA English DT Article DE solute transport; stream flow; modeling; transient storage; hyporheic ID LONGITUDINAL SOLUTE TRANSPORT; MASS-TRANSFER; FLOW-RATES; EXCHANGE; TRACER; PARAMETERS; MODEL; ZONE; MORPHOLOGY; RETENTION AB The accumulation of discharge along a stream valley is frequently assumed to be the primary control on solute transport processes. Relationships of both increasing and decreasing transient storage, and decreased gross losses of stream water have been reported with increasing discharge; however, we have yet to validate these relationships with extensive field study. We conducted transient storage and mass recovery analyses of artificial tracer studies completed for 28 contiguous 100 m reaches along a stream valley, repeated under four base-flow conditions. We calculated net and gross gains and losses, temporal moments of tracer breakthrough curves, and best fit transient storage model parameters (with uncertainty estimates) for 106 individual tracer injections. Results supported predictions that gross loss of channel water would decrease with increased discharge. However, results showed no clear relationship between discharge and transient storage, and further analysis of solute tracer methods demonstrated that the lack of this relation may be explained by uncertainty and equifinality in the transient storage model framework. Furthermore, comparison of water balance and transient storage approaches reveals complications in clear interpretation of either method due to changes in advective transport time, which sets a the temporal boundary separating transient storage and channel water balance. We have little ability to parse this limitation of solute tracer methods from the physical processes we seek to study. We suggest the combined analysis of both transient storage and channel water balance more completely characterizes transport of solutes in stream networks than can be inferred from either method alone. C1 [Ward, Adam S.] Univ Iowa, Dept Geosci, Iowa City, IA 52242 USA. [Payn, Robert A.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Gooseff, Michael N.; Kelleher, Christa A.; Wagener, Thorsten] Penn State Univ, Dept Civil & Environm Engn, University Pk, PA 16802 USA. [McGlynn, Brian L.] Duke Univ, Nicholas Sch Environm, Div Earth & Ocean Sci, Durham, NC 27708 USA. [Bencala, Kenneth E.] US Geol Survey, Menlo Pk, CA 94025 USA. [Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. [Wagener, Thorsten] Univ Bristol, Dept Civil Engn, Bristol, Avon, England. RP Ward, AS (reprint author), Univ Iowa, Dept Geosci, 36 Trowbridge Hall, Iowa City, IA 52242 USA. EM adam-ward@uiowa.edu RI McGlynn, Brian/A-2509-2008; Ward, Adam/H-7668-2012; Wagener, Thorsten/C-2062-2008; Gooseff, Michael/N-6087-2015 OI McGlynn, Brian/0000-0001-5266-4894; Ward, Adam/0000-0002-6376-0061; Wagener, Thorsten/0000-0003-3881-5849; Gooseff, Michael/0000-0003-4322-8315 FU National Science Foundation's Hydrologic Sciences program [EAR-0911435] FX We thank the personnel at the U.S. Forest Service Rocky Mountain Research Station, Tenderfoot Creek Experimental Forest for their support. This material is based upon work supported by the National Science Foundation's Hydrologic Sciences program, under grant no. EAR-0911435. Any opinions, findings, and conclusion or recommendations expressed in this document are those of the authors and do not necessarily reflect the views of the National Science Foundation, U. S. Geological Survey, or U. S. Forest Service. Our thanks to Judson Harvey, two anonymous reviewers, and the Associate Editor, all of whom provided comments that improved the quality of this manuscript and furthered our thinking on the subject. NR 53 TC 24 Z9 26 U1 2 U2 44 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD JUN PY 2013 VL 49 IS 6 BP 3359 EP 3374 DI 10.1002/wrcr.20148 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 189BF UT WOS:000322241300020 ER PT J AU Povak, NA Hessburg, PF Reynolds, KM Sullivan, TJ McDonnell, TC Salter, RB AF Povak, Nicholas A. Hessburg, Paul F. Reynolds, Keith M. Sullivan, Timothy J. McDonnell, Todd C. Salter, R. Brion TI Machine learning and hurdle models for improving regional predictions of stream water acid neutralizing capacity SO WATER RESOURCES RESEARCH LA English DT Article DE Acid neutralizing capacity; ANC; machine learning; stream water acidification; imbalanced data; sulfur deposition ID CONTERMINOUS UNITED-STATES; SURFACE WATERS; CLIMATE-CHANGE; SPECIES DISTRIBUTION; CHEMICAL-PROCESSES; ADIRONDACK REGION; REGRESSION TREES; CRITICAL LOADS; SOIL-WATER; NEW-YORK AB In many industrialized regions of the world, atmospherically deposited sulfur derived from industrial, nonpoint air pollution sources reduces stream water quality and results in acidic conditions that threaten aquatic resources. Accurate maps of predicted stream water acidity are an essential aid to managers who must identify acid-sensitive streams, potentially affected biota, and create resource protection strategies. In this study, we developed correlative models to predict the acid neutralizing capacity (ANC) of streams across the southern Appalachian Mountain region, USA. Models were developed using stream water chemistry data from 933 sampled locations and continuous maps of pertinent environmental and climatic predictors. Environmental predictors were averaged across the upslope contributing area for each sampled stream location and submitted to both statistical and machine-learning regression models. Predictor variables represented key aspects of the contributing geology, soils, climate, topography, and acidic deposition. To reduce model error rates, we employed hurdle modeling to screen out well-buffered sites and predict continuous ANC for the remainder of the stream network. Models predicted acid-sensitive streams in forested watersheds with small contributing areas, siliceous lithologies, cool and moist environments, low clay content soils, and moderate or higher dry sulfur deposition. Our results confirmed findings from other studies and further identified several influential climatic variables and variable interactions. Model predictions indicated that one quarter of the total stream network was sensitive to additional sulfur inputs (i.e., ANC< 100 mu eq L-1), while < 10% displayed much lower ANC (< 50 mu eq L-1). These methods may be readily adapted in other regions to assess stream water quality and potential biotic sensitivity to acidic inputs. C1 [Povak, Nicholas A.; Hessburg, Paul F.; Salter, R. Brion] US Forest Serv, Wenatchee Forestry Sci Lab, Pacific NW Res Stn, Wenatchee, WA 98801 USA. [Reynolds, Keith M.] US Forest Serv, Corvallis Forestry Sci Lab, Pacific NW Res Stn, Corvallis, OR 98801 USA. [Sullivan, Timothy J.; McDonnell, Todd C.] E&S Environm Chem Inc, Corvallis, OR USA. RP Povak, NA (reprint author), US Forest Serv, Wenatchee Forestry Sci Lab, Pacific NW Res Stn, Wenatchee, WA 98801 USA. EM npovak@fs.fed.us OI Reynolds, Keith/0000-0002-5286-4754 NR 95 TC 6 Z9 6 U1 1 U2 16 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD JUN PY 2013 VL 49 IS 6 BP 3531 EP 3546 DI 10.1002/wrcr.20308 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 189BF UT WOS:000322241300031 ER PT J AU Vishwanathan, R Chung, M Johnson, EJ AF Vishwanathan, Rohini Chung, Mei Johnson, Elizabeth J. TI A Systematic Review on Zinc for the Prevention and Treatment of Age-Related Macular Degeneration SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Review DE zinc; age-related macular degeneration; systematic review ID VISUAL-ACUITY MEASUREMENTS; DIETARY ANTIOXIDANTS; CLINICAL-TRIAL; ORAL ZINC; EYE; MACULOPATHY; RISK; METAANALYSIS; HEALTH AB PURPOSE. The objective of this systematic review was to examine the evidence on zinc intake from foods and supplements in the primary prevention and treatment of AMD. METHODS. Randomized controlled trials (RCTs), prospective cohort, retrospective cohort, and case-control studies that investigated zinc intake from foods and/or supplements, and AMD in men and women with a mean age of 50 years or older were included. Medline and Cochrane Central were searched from inception to February 2012 and November 2012, respectively. Data extraction and quality appraisal were done on all eligible studies. RESULTS. Ten studies were included: four RCTs, four prospective cohort, and two retrospective cohort studies. Age-related Eye Disease Study (AREDS) showed zinc treatment to significantly reduce the risk of progression to advanced AMD. The risk of visual acuity loss was of similar magnitude, but not statistically significant. Two RCTs reported statistically significant increases in visual acuity in early AMD patients and one RCT showed no effect of zinc treatment on visual acuity in advanced AMD patients. Results from six cohort studies on associations between zinc intake and incidence of AMD were inconsistent. CONCLUSIONS. Current evidence on zinc intake for the prevention of AMD is inconclusive. Based on the strength of AREDS, we can conclude that zinc treatment may be effective in preventing progression to advanced AMD. Zinc supplementation alone may not be sufficient to produce clinically meaningful changes in visual acuity. C1 [Vishwanathan, Rohini; Johnson, Elizabeth J.] Tufts Univ, Carotenoids & Hlth Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chung, Mei] Tufts Univ, Nutr Infect Unit, Dept Publ Hlth & Community Med, Sch Med, Boston, MA 02111 USA. RP Vishwanathan, R (reprint author), Tufts Univ, Carotenoids & Hlth Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM rohini.vishwanathan@tufts.edu FU United States Department of Agriculture [1959051000-073-025]; Bausch Lomb, Inc. FX Support by grants from the United States Department of Agriculture 1959051000-073-025 and Bausch & Lomb, Inc. NR 33 TC 15 Z9 15 U1 3 U2 4 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 EI 1552-5783 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD JUN PY 2013 VL 54 IS 6 BP 3985 EP 3998 DI 10.1167/iovs.12-11552 PG 14 WC Ophthalmology SC Ophthalmology GA 173YW UT WOS:000321120700021 PM 23652490 ER PT J AU Adel, MN Pourbabaei, H Omidi, A Dey, DC AF Adel, Mohammad Naghi Pourbabaei, Hassan Omidi, Ali Dey, Daniel C. TI Forest structure and woody plant species composition after a wildfire in beech forests in the north of Iran SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE fire; structure; composition; regeneration; beech forest; Guilan Province ID PONDEROSA PINE FOREST; MIXED-CONIFER FOREST; PRESCRIBED FIRE; UNDERSTORY VEGETATION; HERBACEOUS VEGETATION; POPULATION-DYNAMICS; COMMUNITY STRUCTURE; SIERRA-NEVADA; INTENSITY; REGENERATION AB Beech (Fagus orientalis Lipsky) forest covers about 565,000 ha of land in Guilan province, north of Iran and forms a major carbon pool. It is an important economic, soil protection and recreation resource. We studied long-term effects of fire on the structure and composition 37 years after fire occurrence in these forests. To do this research, we selected 85 ha burned and 85 ha unburned beech forests). The results indicated that the fire had not changed the overall uneven-aged structure, but it changed forest composition from pure stands to mixed stands that now include species such as Carpinus betulus, Acer cappadocicum and Alnus subcordata. The density of trees and regeneration was significantly increased, while the density of shrubs significantly decreased. The main reasons for increased tree regeneration were attributed to (1) reduction of litter depth, and (2) increase in available light from opening of the canopy and reduction in shrub competition. It is apparent that the forest is on a path to return to its natural state before the fire after 37 years. C1 [Adel, Mohammad Naghi; Pourbabaei, Hassan] Univ Guilan, Nat Resources Fac, Fac Dept Forestry, Somehsara, Guilan, Iran. [Omidi, Ali] Nat Resources Off, Dept Forestry, Guilan, Iran. [Dey, Daniel C.] US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP Adel, MN (reprint author), Univ Guilan, Nat Resources Fac, Fac Dept Forestry, POB 1144, Somehsara, Guilan, Iran. EM mn.adel87@yahoo.com NR 62 TC 4 Z9 5 U1 1 U2 10 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO. 26 HEXING RD, DONGLI DISTRICT, HARBIN, 00000, PEOPLES R CHINA SN 1007-662X J9 J FORESTRY RES JI J. For. Res. PD JUN PY 2013 VL 24 IS 2 BP 255 EP 262 DI 10.1007/s11676-012-0316-7 PG 8 WC Forestry SC Forestry GA 188KC UT WOS:000322190900007 ER PT J AU Lawson, SS Pijut, PM Michler, CH AF Lawson, Shaneka S. Pijut, Paula M. Michler, Charles H. TI Species selection in hardwoods research: variations in baseline physiological responses of select temperate hardwood tree species SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE drought; water stress; stomatal density; hardwood trees ID MAXIMUM STOMATAL CONDUCTANCE; NORTH-AMERICA; ARABIDOPSIS-THALIANA; CO2 CONCENTRATION; ATMOSPHERIC CO2; PLANT-RESPONSES; CLIMATE-CHANGE; DENSITY; WATER; DROUGHT AB Drought periods are becoming more extreme worldwide and the ability of plants to contribute towards atmospheric flux is being compromised. Properly functioning stomata provide an exit for water that has been absorbed by the roots, funneled into various cell parts, and eventually released into the atmosphere via transpiration. By observing the effects that weather conditions such as climate change may have on stomatal density, distribution, and functioning, it may be possible to elucidate a portion of the mechanisms trees use to survive longer periods of water stress. This study analyzed stomatal density (SD), stomatal conductance (g(s)), CO2 assimilation (A), instantaneous water-use efficiency (WUEi), and transpiration (E) rates in six native tree species in the Midwestern USA and showed that trees within the same ecotype followed similar trends, but that trees within the same family did not when exposed to identical greenhouse conditions. Naturally drought tolerant tree species demonstrated lower g(s) and higher WUEi, while intolerant species had higher SD. This study showed negative or no correlation between SD and g(s), A, E, and WUEi and positive correlations between E and A and g(s) and E. C1 [Lawson, Shaneka S.] Purdue Univ, USDA, Forest Serv, Northern Res Stn,HTIRC,FORS309,Dept Forestry & Na, W Lafayette, IN 47907 USA. [Pijut, Paula M.; Michler, Charles H.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. RP Lawson, SS (reprint author), Purdue Univ, USDA, Forest Serv, Northern Res Stn,HTIRC,FORS309,Dept Forestry & Na, 715 West State St, W Lafayette, IN 47907 USA. EM sslawson@fs.fed.us RI Pijut, Paula/N-6789-2015 FU Fred M. van Eck Foundation; Alliance for Graduate Education and Professoriate (AGEP) at Purdue University FX We would like to thank Drs Martin-Michel Gauthier, Michael Jenkins, and John Gordon for revising an earlier version of the manuscript. We would also like to thank Caleb Brown and Dr. Douglass Jacobs for equipment and technical training in the greenhouse. This study was financed by fellowships from the Fred M. van Eck Foundation and the Alliance for Graduate Education and Professoriate (AGEP) at Purdue University. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the US Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 46 TC 0 Z9 0 U1 3 U2 21 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD JUN PY 2013 VL 24 IS 2 BP 285 EP 292 DI 10.1007/s11676-013-0351-z PG 8 WC Forestry SC Forestry GA 188KC UT WOS:000322190900011 ER PT J AU Borak, B Ort, DR Burbaum, JJ AF Borak, Brian Ort, Donald R. Burbaum, Jonathan J. TI Energy and carbon accounting to compare bioenergy crops SO CURRENT OPINION IN BIOTECHNOLOGY LA English DT Review ID SOLAR-RADIATION; PHOTOSYNTHESIS; EFFICIENCY; PLANTS; YIELD; C-3; OPPORTUNITIES; PRODUCTIVITY; CHALLENGES; EVOLUTION AB To compare the utility of current and future biofuels and biofuel feedstocks in an objective manner can be extremely challenging. This challenge exists because agricultural data are inherently variable, experimental techniques are crop-dependent, and the literatures usually report relative, rather than absolute, values. Here, we discuss the 'PETRO approach', a systematic approach to evaluate new crops. This approach accounts for not only the capture of solar energy but also the capture of atmospheric carbon (as CO2) to generate a final carbon-based liquid fuel product. The energy yield, per unit area, of biofuel crops grown in different climate zones can thus be benchmarked and quantitatively compared in terms of both carbon gain and solar energy conversion efficiency. C1 [Borak, Brian] Booz Allen Hamilton, Washington, DC 20024 USA. [Ort, Donald R.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Burbaum, Jonathan J.] US DOE, Adv Res Projects Agcy Energy, Washington, DC 20585 USA. RP Burbaum, JJ (reprint author), US DOE, Adv Res Projects Agcy Energy, Washington, DC 20585 USA. EM jonathan.burbaum@hq.doe.gov NR 34 TC 7 Z9 7 U1 1 U2 34 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0958-1669 J9 CURR OPIN BIOTECH JI Curr. Opin. Biotechnol. PD JUN PY 2013 VL 24 IS 3 BP 369 EP 375 DI 10.1016/j.copbio.2013.02.018 PG 7 WC Biochemical Research Methods; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 170DG UT WOS:000320829500002 PM 23518005 ER PT J AU Skinner, RH AF Skinner, R. Howard TI Nitrogen fertilization effects on pasture photosynthesis, respiration, and ecosystem carbon content SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Ecosystem respiration; Gross primary productivity; Pastures; N fertilization; Eddy covariance ID SOIL CARBON; ELEVATED CO2; ENVIRONMENTAL-CONDITIONS; GRASSLAND ECOSYSTEMS; EDDY COVARIANCE; SEQUESTRATION; MANAGEMENT; CLIMATE; FLUX; SWITCHGRASS AB Some studies have shown that increasing N fertility can increase soil C sequestration, whereas others suggest that N fertilization has no effect on sequestration. Increasing N fertilization typically increases annual photosynthetic C uptake (gross primary productivity or GPP) and forage yield but also increases ecosystem respiration (Re), such that net ecosystem exchange (NEE) can increase, remain unchanged, or decrease depending on the relative impact of the fertilizer application on these two competing processes. Nitrogen fertilization also affects other inputs and outputs to the systems such as forage removal and manure deposition. A nine-year study monitoring carbon dioxide flux at two pasture sites examined in detail the effects of N fertilization rate on NEE, GPP and Re under a range of environmental conditions. High N fertility (>200 kg N ha(-1) yr(-1)) increased yield over a low N fertility site (<100 kg N ha(-1) yr(-1)) by 38%. As expected, forage yield was significantly correlated with GPP (P = 0.01), but also with Re (P = 0.003). No significant relationship existed between NEE and either GPP or Re. A nearly 1:1 relationship existed between GPP and Re over a wide range of environmental and fertilizer input conditions. For the high-N pasture, the net loss of C from the ecosystem increased under increased N fertilization because of the greater forage yield and subsequent removal of harvested C. Increasing N fertilizer application rate did not increase soil C sequestration in this temperate pasture system. Published by Elsevier B.V. C1 USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Skinner, RH (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. EM howard.skinner@ars.usda.gov NR 37 TC 8 Z9 9 U1 5 U2 89 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD JUN 1 PY 2013 VL 172 BP 35 EP 41 DI 10.1016/j.agee.2013.04.005 PG 7 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA 178XJ UT WOS:000321480400005 ER PT J AU de Carvalho, LB Alves, PLCA Duke, SO AF de Carvalho, Leonardo B. Alves, Pedro L. C. A. Duke, Stephen O. TI Hormesis with glyphosate depends on coffee growth stage SO ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS LA English DT Article DE Coffea arabica L.; weed management; herbicide; spray drift; stimulatory effect ID RYEGRASS LOLIUM-MULTIFLORUM; PLANTS; SUGARCANE; CULTIVARS; METABOLISM; MECHANISMS; RESISTANCE; QUALITY; L. AB Weed management systems in almost all Brazilian coffee plantations allow herbicide spray to drift on crop plants. In order to evaluate if there is any effect of the most commonly used herbicide in coffee production, glyphosate, on coffee plants, a range of glyphosate doses were applied directly on coffee plants at two distinct plant growth stages. Although growth of both young and old plants was reduced at higher glyphosate doses, low doses caused no effects on growth characteristics of young plants and stimulated growth of older plants. Therefore, hormesis with glyphosate is dependent on coffee plant growth stage at the time of herbicide application. C1 [de Carvalho, Leonardo B.] Univ Estado Santa Catarina, Dept Agron, Ctr Ciencias Agrovet, BR-88520000 Lages, SC, Brazil. [Alves, Pedro L. C. A.] Univ Estadual Paulista, Dept Biol Aplicada Agr, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil. [Duke, Stephen O.] Univ Mississippi, ARS, USDA, Nat Prod Utilizat Res Unit, Oxford, MS 38677 USA. RP de Carvalho, LB (reprint author), Univ Estado Santa Catarina, Dept Agron, Ctr Ciencias Agrovet, Av Luiz de Camoes 2090, BR-88520000 Lages, SC, Brazil. EM lbcarvalho@cav.udesc.br RI Carvalho, Leonardo/C-2330-2009; Alves, Pedro Luis/D-1305-2012 OI Carvalho, Leonardo/0000-0001-8110-3471; Alves, Pedro Luis/0000-0003-2348-2121 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX We would like to thank Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for the doctorate scholarship provided to the first author. NR 37 TC 7 Z9 7 U1 1 U2 7 PU ACAD BRASILEIRA DE CIENCIAS PI RIO JANEIRO PA RUA ANFILOFIO DE CARVALHO, 29, 3 ANDAR, 20030-060 RIO JANEIRO, BRAZIL SN 0001-3765 J9 AN ACAD BRAS CIENC JI An. Acad. Bras. Cienc. PD JUN PY 2013 VL 85 IS 2 BP 813 EP 821 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177SI UT WOS:000321395300033 PM 23828346 ER PT J AU Shelly, T Renshaw, J Dunivin, R Morris, T Giles, T Andress, E Diaz, A War, M Nishimoto, J Kurashima, R AF Shelly, Todd Renshaw, John Dunivin, Robin Morris, Terri Giles, Tisha Andress, Earl Diaz, Archie War, Mamadou Nishimoto, Jon Kurashima, Rick TI RELEASE-RECAPTURE OF BACTROCERA FRUIT FLIES (DIPTERA: TEPHRITIDAE): COMPARING THE EFFICACY OF LIQUID AND SOLID FORMULATIONS OF MALE LURES IN FLORIDA, CALIFORNIA AND HAWAII SO FLORIDA ENTOMOLOGIST LA English DT Article DE fruit fly; detection; trapping; methyl eugenol; cue-lure ID PARAPHEROMONE-BAITED TRAPS; FLY SPECIES DIPTERA; METHYL EUGENOL; CUE-LURE; CUCURBITAE DIPTERA; INSECTICIDE DISPENSERS; CERATITIS-CAPITATA; SEXUAL-MATURATION; MALE ANNIHILATION; RASPBERRY KETONE AB Invasive species of Bactrocera fruit flies (Diptera: Tephritidae), particularly B. dorsalis (Hendel) and B. cucurbitae (Coquillett), pose serious threats to agricultural crops. Detection relies largely on traps baited with the male lures methyl eugenol (ME), which is attractive to B. dorsalis, and cue-lure (CL) or the related chemicals raspberry ketone (RK) and raspberry ketone formate (RKF), which are all attractive to B. cucurbitae. Currently, ME and CL are applied as liquids to cotton wicks (along with an insecticide), a procedure involving considerable handling time and exposure to pesticides. Recent studies have shown that traps baited with solid dispensers (plugs or wafers) of male lures, which arrive in sealed envelopes ready for use, catch at least as many Bactrocera males as traps baited with liquid lures. The present study compared captures of B. dorsalis and B. cucurbitae males in traps baited with liquid lures versus traps baited with lure-bearing solid dispensers in Florida, California, and Hawaii. In the first 2 locations, marked, irradiated males were released at street intersections, and captures were scored at 4 trapping stations 50 m distant along the intersecting streets with various lure/dispenser combinations. In Hawaii, trap catch of wild B. dorsalis and B. cucurbitae were compared among traps with liquid and solid formulations of the lures. Although several exceptions were observed, the overall finding was that the lure-bearing plugs and wafers captured as many or more Bactrocera males as the liquid application. Consequently, we suggest that solid dispensers could be adopted in area-wide fruit fly surveillance programs without lessening their detection sensitivity to incipient infestations. The use of solid dispensers that contain both ME and RK, in particular, could greatly reduce the number of traps required and result in considerable cost savings. C1 [Shelly, Todd; Nishimoto, Jon; Kurashima, Rick] USDA APHIS, Waimanalo, HI 96795 USA. [Renshaw, John; Dunivin, Robin; Morris, Terri; Giles, Tisha] USDA APHIS, Sarasota, FL 34243 USA. [Andress, Earl] USDA APHIS, Los Alamitos, CA 90720 USA. [Diaz, Archie] CDFA, Anaheim, CA 92801 USA. [War, Mamadou] CDFA, Los Alamitos, CA 90720 USA. RP Shelly, T (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM tshelly34@yahoo.com NR 35 TC 2 Z9 2 U1 2 U2 22 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 305 EP 317 PG 13 WC Entomology SC Entomology GA 168VY UT WOS:000320737200001 ER PT J AU Ciomperlik, MA Robinson, DG Gibbs, IH Fields, A Stevens, T Taylor, BM AF Ciomperlik, Matthew A. Robinson, David G. Gibbs, Ian H. Fields, Angela Stevens, Timothy Taylor, Bret M. TI MORTALITY TO THE GIANT AFRICAN SNAIL, LISSACHATINA FULICA (GASTROPODA: ACHATINIDAE), AND NON-TARGET SNAILS USING SELECT MOLLUSCICIDES SO FLORIDA ENTOMOLOGIST LA English DT Article DE diatomaceous earth; kaolin; non-target snail species; mortality; bioassay; Barbados ID ANGIOSTRONGYLUS-CANTONENSIS; EOSINOPHILIC-MENINGITIS; RATS; OUTBREAK AB Laboratory bioassays and caged field trials were conducted to compare the acute toxicities of molluscicide formulations on the neonate, juvenile, and adult development stages of giant African snail (GAS) Lissachatina fulica (Bowdich 1822) and 3 non-target snail species in Barbados. Nine commercially available molluscicides, diatomaceous earth, and a kaolin clay product (Surround WP) were evaluated. High levels of mortality to neonate GAS were seen in all the laboratory molluscicide bioassays except for Surround and diatomaceous earth. The highest mortality rates to neonate GAS were observed from Durham granules and Slugfest treatments tested during field trials. Deadline, Durham granules, Metarex, and Orcal pellets caused the highest rates of mortality to juvenile GAS in our field trials. For adult GAS several molluscicides including Blitzem, Deadline, Durham Granules, Mesurol 75W, Metarex, Orcal pellet, and Slugfest caused greater than 95 % mortality in laboratory bioassays. Field trials showed that Durham granules and Slugfest yielded the highest mortality rates. Sluggo pellet, touted as an environmentally safe molluscicide, did not cause high rates of mortality to juvenile and adult GAS in our field trials, but did cause higher rates of mortality to neonate GAS than the control. The majority of the molluscicides tested in our trials were equally or more lethal to 3 non-target snail species than GAS. Our results identify several effective commercially available molluscicides that can be used to control incipient populations of GAS. However, our results show that the potential impact on non-target snail species during control or eradication programs may be considerable, causing substantial mortality regardless of what brand, active ingredient, or formulation is used. C1 [Ciomperlik, Matthew A.] USDA, Ctr Plant Hlth Sci & Technol, Mission Lab, Edinburg, TX 78541 USA. [Robinson, David G.] Acad Nat Sci Philadelphia, USDA APHIS PPQ NIS, Philadelphia, PA 19103 USA. [Gibbs, Ian H.; Taylor, Bret M.] Minist Agr Food Fisheries & Water Resource Manage, Christ Church, Barbados. [Fields, Angela] Univ W Indies, Bridgetown, Barbados. [Stevens, Timothy] USDA APHIS PPQ Eastern Reg, Wilmington, NC 28405 USA. RP Ciomperlik, MA (reprint author), USDA, Ctr Plant Hlth Sci & Technol, Mission Lab, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. EM Matt.A.Ciomperlik@aphis.usda.gov NR 35 TC 1 Z9 1 U1 4 U2 20 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 370 EP 379 PG 10 WC Entomology SC Entomology GA 168VY UT WOS:000320737200009 ER PT J AU Richardson, ML Hall, DG AF Richardson, Matthew L. Hall, David G. TI TOXICITY OF 6 MITICIDES TO THE ASIAN CITRUS PSYLLID, DIAPHORINA CITRI (HEMIPTERA: LIVIIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Article DE bifenazate; chlorfenapyr; dicofol; petroleum oil; pyridaben; spirodiclofen ID TETRANYCHUS-URTICAE; MITES ACARI; DISEASE AB The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important pest of citrus. Research into strategies to control ACP is ongoing at many facilities, including at the USDA-ARS U.S. Horticultural Research Laboratory (USHRL) in Fort Pierce, Florida. The USHRL maintains colonies of ACP, but their survival is often threatened by mites which render host plants unsuitable for ACP. Our objective was to identify miticides/insecticides that could be used to control mite outbreaks with minimal or no adverse affect on ACP. We tested the following 6 miticides in greenhouse trials for their toxicity to each life stage of ACP and also investigated sublethal effects on development and oviposition of ACP: bifenazate (Acramite 50 WS), spirodiclofen (Envidor 2 SC), dicofol (Kelthane MF), pyridaben (Nexter), petroleum oil, and chlorfenapyr (Pylon). The miticides differed in their toxicity when applied directly to ACP. Bifenazate was the only miticide that was not toxic to any life stage of ACP, whereas pyridaben and chlorfenapyr, which are also labeled as insecticides, were toxic to all life stages of ACP. Petroleum oil and dicofol killed adult and nymphal ACP, but were nontoxic to eggs. Spirodiclofen was nontoxic to adults, but reduced nymphal survivorship and killed eggs. The duration of residual activity against adult ACP also was widely variable: dicofol residues were only toxic for up to 10 days, whereas chlorfenapyr residues were still toxic after 36 days. We recommend using dicofol, pyridaben, petroleum oil, and Chlorfenapyr to maintain clean plants prior to colonization by ACP and then rotating bifenazate and spirodiclofen, if maintaining adult ACP, or bifenazate, dicofol, and petroleum oil, if maintaining eggs. Bifenazate is the only product safe for maintaining nymphal ACP. Our results are useful for research facilities that wish to maintain colonies of ACP and control mites and may be useful for citrus growers and researchers that wish to kill ACP and mites with a single treatment. C1 [Richardson, Matthew L.; Hall, David G.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34951 USA. RP Hall, DG (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34951 USA. EM David.Hall@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 21 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 433 EP 441 PG 9 WC Entomology SC Entomology GA 168VY UT WOS:000320737200017 ER PT J AU Horton, DR Lewis, TM Dobbs, TT AF Horton, David R. Lewis, Tamera M. Dobbs, Thomas T. TI INTERCEPTIONS OF ANTHOCORIDAE, LASIOCHILIDAE, AND LYCTOCORIDAE AT THE MIAMI PLANT INSPECTION STATION (HEMIPTERA: HETEROPTERA) SO FLORIDA ENTOMOLOGIST LA English DT Article DE minute pirate bugs; flower bugs; port interceptions; non-indigenous species; Anthocoridae ID BUCHANANIELLA-CONTINUA WHITE; NORTH-AMERICAN RECORDS; REUTER HEMIPTERA; KOREAN PENINSULA; UNITED-STATES; HAWAIIAN-ISLANDS; GENUS XYLOCORIS; CIMICOIDEA; INSECTA; JAPAN AB Specimens of Anthocoridae, Lyctocoridae, and Lasiochilidae (Hemiptera: Heteroptera) intercepted at various ports-of-entry and housed at the Animal and Plant Health Inspection Services (APHIS) Miami Plant Inspection Station (Miami, FL) were examined and identified to species or genus. The collection comprised 127 specimens intercepted primarily at the Miami Inspection Station. Specimens were distributed among 14 genera and 26 identified species in 3 families: Anthocoridae (99 specimens), Lyctocoridae (9 specimens), and Lasiochilidae (19 specimens). Seventy-eight of the 127 specimens could be identified to species. The remaining 49 specimens were identified to genus, except for 2 specimens that could not be identified below tribal level. For each identified species, we provide brief descriptions of habitat and prey preferences (where known), and a summary of currently known geographic range. Fifty-six of the 127 specimens were of a single genus: Onus Wolff, 1811 (Anthocoridae: Oriini). The specimens of Onus comprised at least 9 different species; 17 specimens could not be identified to species. The 127 specimens were intercepted on a variety of commodities, including ornamental plants, cut flowers, bouquets, agricultural produce, ceramic tiles, and wood products. Fourteen of the identified species do not currently occur in the continental U.S.; moreover, the 49 specimens that we could identify only to genus very likely also are of species not currently established in the continental U.S. The majority of intercepted specimens (93 of 127) arrived on shipments from the Neotropics and Europe. Specimens of Lasiochilidae and Scolopini (Anthocoridae) were entirely from shipments arriving from the Neotropical region. Specimens of Onus were intercepted on shipments from the Neotropics, Mexico, Europe, and Sub-Saharan Africa. Finally, 10 species were intercepted on shipments arriving from countries not previously listed as being part of their known geographic ranges. One Old World species, Cardiastethus affinis Poppius, 1909, known previously only from East Africa and India, was intercepted in 2 separate shipments arriving from Central America and the West Indies. C1 [Horton, David R.; Lewis, Tamera M.] USDA ARS, Wapato, WA 98951 USA. [Dobbs, Thomas T.] USDA, Anim & Plant Hlth Inspect Serv, Miami Inspect Stn, Miami, FL 33159 USA. RP Horton, DR (reprint author), USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM david.horton@ars.usda.gov NR 85 TC 2 Z9 2 U1 0 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 482 EP 497 PG 16 WC Entomology SC Entomology GA 168VY UT WOS:000320737200024 ER PT J AU Ruiz-Najera, RE Ruiz-Estudillo, RA Sanchez-Yanez, JM Molina-Ochoa, J Skoda, SR Coutino-Ruiz, R Pinto-Ruiz, R Guevara-Hernandez, F Foster, JE AF Ruiz-Najera, Ramiro E. Ruiz-Estudillo, Ramiro A. Sanchez-Yanez, Juan M. Molina-Ochoa, Jaime Skoda, Steven R. Coutino-Ruiz, Roberto Pinto-Ruiz, Rene Guevara-Hernandez, Francisco Foster, John E. TI OCCURRENCE OF ENTOMOPATHOGENIC FUNGI AND PARASITIC NEMATODES ON SPODOPTERA FRUGIPERDA (LEPIDOPTERA: NOCTUIDAE) LARVAE COLLECTED IN CENTRAL CHIAPAS, MEXICO SO FLORIDA ENTOMOLOGIST LA English DT Article DE Fall armyworm; natural enemies; mermithid nematodes; entomopathogenic microorganisms; corn ID FALL ARMYWORM LEPIDOPTERA; POPULATION; PATHOGENS AB Fall armyworm larvae (FAW), Spodoptera frugiperda (J. E. Smith) were collected from whorl-stage corn, Zea mays (L.), between the V-2 and V-4 stages, in 22 localities' of Central, Chiapas, Mexico, called "La Frailesca" during late Jun 2009 to determine the occurrence of native entomopathogens and parasitic nematodes, and to determine the most parasitized instars. A total of 1,247 larvae were examined in search of native biological control agents. Overall total larval mortality was 16.36%. The percent larval mortality due to entomopathogens and parasitic nematodes was 12.99%. Hexamermis sp., probably albicans Siebold (Mermitha: Mermithidae) were isolated from 105 FAW larvae (8.42%) with highest incidence from the 4th, 5th, 6th and 3rd instars, respectively. A hyphomycete, Nomuraea rileyi Farlow (Samson), was recovered from 38 larvae (about 3.05% parasitism) with highest incidence in the 5th, 3rd, 4th, and 6th instars, respectively. Lowest percentage of FAW larval parasitism was caused by unidentified microsporidian or microsporidium (1.52%), infecting 19 larvae in the 6th, 5th and 3rd instars, respectively. First and 2nd instars did not show parasitism. This information is useful in designing future biological control programs. C1 [Ruiz-Najera, Ramiro E.; Coutino-Ruiz, Roberto; Pinto-Ruiz, Rene; Guevara-Hernandez, Francisco] Univ Autonoma Chiapas, Fac Ciencias Agron, Entomol Lab, Villaflores 30470, Chiapas, Mexico. [Ruiz-Estudillo, Ramiro A.; Molina-Ochoa, Jaime] Univ Autonoma Chiapas, Fac Ciencias Agron, Villaflores, Chiapas, Mexico. [Sanchez-Yanez, Juan M.] Univ Michoacana, Lab Microbiol Ambiental, Inst Invest Quim Biol, Morelia 58140, Michoacan, Mexico. Univ Colima, Secretaria Invest, Colima 28040, Mexico. [Skoda, Steven R.] USDA ARS, KBUSLIRL Screwworm Res Unit, Kerrville, TX 78028 USA. [Molina-Ochoa, Jaime; Foster, John E.] Univ Nebraska, Dept Entomol, Insect Genet Lab, Lincoln, NE 68583 USA. RP Molina-Ochoa, J (reprint author), Univ Autonoma Chiapas, Fac Ciencias Agron, Campus 5,Apartado Postal 78, Villaflores, Chiapas, Mexico. EM jmolina@ucol.mx NR 28 TC 1 Z9 1 U1 3 U2 20 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 498 EP 503 PG 6 WC Entomology SC Entomology GA 168VY UT WOS:000320737200025 ER PT J AU Sharma, S Oi, DH Buss, EA AF Sharma, Shweta Oi, David H. Buss, Eileen A. TI HONEYDEW-PRODUCING HEMIPTERANS IN FLORIDA ASSOCIATED WITH NYLANDERIA FULVA (HYMENOPTERA: FORMICIDAE), AN INVASIVE CRAZY ANT SO FLORIDA ENTOMOLOGIST LA English DT Article DE Paratrechina pubens; tawny crazy ant; Caribbean crazy ant; Rasberry crazy ant; Hemiptera; invasive ants; carton shelter ID SOLENOPSIS-INVICTA; MUTUALISM; HOMOPTERA; PEST; TEXAS; TREE AB Nylanderia fulva (Mayr) (Formicidae) is an invasive pest ant that has been reported in Florida, Texas, Louisiana and Mississippi. Workers tend various honeydew producing hemipterans in Florida landscapes and natural areas. We sought to understand the seasonal foraging activities of N. fulva and its relationship with honeydew producing hemipterans. Twenty-two hemipteran species were collected from 15 different plant hosts sampled in Florida from Jul 2010 to Jul 2012. The relative density of hemipterans on 4 plant species Dive oak (Quercus virginiana Mill.; Fagales: Fagaceae), holly (Ilex cornuta Lindl.; Aquifoliales: Aquifoliaceae), magnolia (Magnolia grandiflora L.; Magnoliales: Magnoliaceae), and sugarberry (Celtis laevigata Willd.; Urticales: Ulmaceae)] was compared to the relative density of N. fulva at the base or stem of the selected plants. The number of N. fulva and hemipterans on each plant species was positively correlated from spring through fall. Both N. fulva and hemipteran populations increased from May to Sep and decreased from Oct to Apr. In addition, Cinara juniperivora (Wilson) (Hemiptera: Aphididae) on southern red cedar and Toumeyella liriodendri (Gmelin) (Hemiptera: Coccidae) on magnolia were covered with carton shelters presumably constructed by N. fulva, suggesting that this ant potentially protects certain hemipterans species. C1 [Sharma, Shweta; Buss, Eileen A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Oi, David H.] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. RP Buss, EA (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM eabuss@ufl.edu FU Tropical and Subtropical Agricultural Research (TSTAR) grant [2010-34135-21096] FX We would like to thank the owners and managers of our study sites in Gainesville for their cooperation and patience. Drs. S. Halbert and I. Stocks (FDACS/DPI) kindly provided species identifications. We appreciate the technical assistance provided by P. Ruppert, M. Poudel, T. Garrick, D. Sekora and S. Rachel during field sampling. Dr. G. Hodges (FDACS/DPI) reviewed an earlier draft of this manuscript. This research was funded by the Tropical and Subtropical Agricultural Research (TSTAR) grant (2010-34135-21096). NR 45 TC 0 Z9 1 U1 6 U2 33 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 EI 1938-5102 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 538 EP 547 PG 10 WC Entomology SC Entomology GA 168VY UT WOS:000320737200029 ER PT J AU Pratt, PD Makinson, J Purcell, M Pogue, MG Rayamajhi, MB Center, TD AF Pratt, Paul D. Makinson, Jeff Purcell, Matthew Pogue, Michael G. Rayamajhi, Min B. Center, Ted D. TI RHODOMYRTUS TOMENTOSA (MYRTALES: MYRTACEAE): NEW HOST RECORDS FOR METANASTRIA GEMELLA (LEPIDOPTERA: LASIOCAMPIDAE) AND ARNA BIPUNCTAPEX (LEPIDOPTERA: LYMANTRIIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE biological control; herbivores; host specificity; invasive species; rose myrtle AB Surveys for host specific natural enemies of Rhodomyrtus tomentosa (Myrtales: Myrtaceae), rose myrtle, in China (mainland and Hong Kong) resulted in the discovery of 2 lepidopteran species that have not been previously reported as herbivores of the plant: Metanastria gemella Lajonquiere (Lasiocampidae) and Arna bipunctapex Hampson (Lymantriidae). Multiple generations of both species were reared on R. tomentosa in a quarantine laboratory, demonstrating that the plant is a developmental host. However, neither species demonstrates sufficient host specificity to be considered for biological control of R. tomentosa in the USA. C1 [Pratt, Paul D.; Rayamajhi, Min B.; Center, Ted D.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Makinson, Jeff; Purcell, Matthew] CSIRO Ecosyst Sci, ARS, USDA, Australian Biol Control Lab, Brisbane, Qld 4001, Australia. [Pogue, Michael G.] ARS, USDA, Systemat Entomol Lab, Smithsonian Inst, Washington, DC 20013 USA. RP Pratt, PD (reprint author), ARS, USDA, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM Paul.Pratt@ars.usda.gov RI Makinson, Jeffrey/M-7031-2014 OI Makinson, Jeffrey/0000-0001-9870-5866 NR 6 TC 4 Z9 4 U1 0 U2 4 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 641 EP 642 PG 2 WC Entomology SC Entomology GA 168VY UT WOS:000320737200045 ER PT J AU Davies, A Sivinski, J Shirk, P Aluja, M AF Davies, Andrew Sivinski, John Shirk, Paul Aluja, Martin TI CLOSELY RELATED WOLBACHIA (RICKETTSIALES: RICKETTSIACEAE) RECOVERED FROM DIFFERENT GENERA OF MEXICAN THELYTOKOUS FIGITIDAE (HYMENOPTERA) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material DE Aganaspis; Odontosema; Anastrepha; Rhagoletis; biological control ID HORIZONTAL TRANSMISSION; REPRODUCTION; PARTHENOGENESIS; TEPHRITIDAE; ARTHROPODS; EULOPHIDAE; PIPIENTIS; PHYLOGENY; INFECTION; SEQUENCES AB Closely related novel Wolbachia strains were recovered from the thelytokous figitids, Odontosema anastrephae Borgmeier and Aganaspis alujai Ovruski et al. No Wolbachia were detected in a bi-sexual strain of O. anastrephae. While the presence or absence of Wolbachia does not demonstrate that Wolbachia is responsible for the lack of males produced by infected females, multilocus sequence typing failed to identify other endosymbionts that might have caused sex-ratio distortions. The phylogenies of insects and their Wolbachia are often not parallel and closely related bacteria infecting apparently more distantly related hosts suggests a horizontal transfer occurred after the divergence of the wasps. Given the figitids present ecology there are few obvious opportunities for transfer through host or habitat-sharing. C1 [Sivinski, John; Shirk, Paul] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. [Aluja, Martin] Inst Ecol AC, Xalapa 91000, Veracruz, Mexico. RP Sivinski, J (reprint author), ARS, USDA, CMAVE, 1600-1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM john.sivinski@ars.usda.gov; alujam@ecologia.edu.mx NR 32 TC 0 Z9 0 U1 2 U2 13 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2013 VL 96 IS 2 BP 649 EP 653 PG 5 WC Entomology SC Entomology GA 168VY UT WOS:000320737200048 ER PT J AU Brose, PH Dey, DC Phillips, RJ Waldrop, TA AF Brose, Patrick H. Dey, Daniel C. Phillips, Ross J. Waldrop, Thomas A. TI A Meta-Analysis of the Fire-Oak Hypothesis: Does Prescribed Burning Promote Oak Reproduction in Eastern North America? SO FOREST SCIENCE LA English DT Article DE fire effects; hardwoods; prescribed fire; Quercus spp.; shelterwood ID SHELTERWOOD STANDS; RED-OAK; HARDWOOD REGENERATION; ADVANCE REGENERATION; CUMBERLAND PLATEAU; COMMUNITY DYNAMICS; QUERCUS-RUBRA; ACER-RUBRUM; PINE FOREST; UPLAND OAK AB The fire-oak hypothesis asserts that the current lack of fire is a reason behind the widespread oak (Quercus spp.) regeneration difficulties of eastern North America, and use of prescribed burning can help solve this problem. We performed a meta-analysis on the data from 32 prescribed fire studies conducted in mixed-oak forests to test whether they supported the latter assertion. Overall, the results suggested that prescribed fire can contribute to sustaining oak forests in some situations, and we identified several factors key to its successful use. Prescribed fire reduced midstory stem density, although this reduction was concentrated in the smaller-diameter stems. Prescribed fire preferentially selected for oak reproduction and against mesophytic hardwood reproduction, but this difference did not translate to an increase in the relative abundance of oak in the advance regeneration pool. Fire equalized the height growth rates of the two species groups. Establishment of new oak seedlings tended to be greater in burned areas than in unburned areas. Generally, prescribed burning provided the most benefit to oak reproduction when the fires occurred during the growing season and several years after a substantial reduction in overstory density. Single fires conducted in closed-canopy stands had little impact in the short term, but multiple burns eventually did benefit oaks in the long term, especially when followed by a canopy disturbance. Finally, we identify several future research needs from our review and synthesis of the fire-oak literature. FOR. SCI. 59(3):322-334. C1 [Brose, Patrick H.] USDA Forest Serv, No Res Stn, Irvine, PA USA. [Dey, Daniel C.; Phillips, Ross J.; Waldrop, Thomas A.] USDA Forest Serv, Irvine, PA USA. RP Brose, PH (reprint author), USDA Forest Serv, No Res Stn, Irvine, PA USA. EM pbrose@fs.fed.us; ddey@fs.fed.us; rjphillips@fs.fed.us; twaldrop@fs.fed.us FU Joint Fire Science Program [10-2-01-1] FX We thank the many fellow scientists who stimulated our thinking on this subject via engaging conversations as well as by sharing insights on the details of their studies, especially data collection procedures, and pointing us toward publications that had escaped our searches. We thank Alejandro Royo, John Stanovick, and Matthew Trager for guidance with the meta-analysis. In addition, we thank them and three anonymous individuals for reviews of earlier drafts of this article that helped with clarity and conciseness. Funding for this study was provided by the Joint Fire Science Program (Project 10-2-01-1). NR 102 TC 38 Z9 39 U1 6 U2 54 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2013 VL 59 IS 3 BP 322 EP 334 DI 10.5849/forsci.12-039 PG 13 WC Forestry SC Forestry GA 174PQ UT WOS:000321168300007 ER PT J AU Headlee, WL Zalesny, RS Hall, RB Bauer, EO Bender, B Birr, BA Miller, RO Randall, JA Wiese, AH AF Headlee, William L. Zalesny, Ronald S., Jr. Hall, Richard B. Bauer, Edmund O. Bender, Bradford Birr, Bruce A. Miller, Raymond O. Randall, Jesse A. Wiese, Adam H. TI Specific Gravity of Hybrid Poplars in the North-Central Region, USA: Within-Tree Variability and Site x Genotype Effects\ SO FORESTS LA English DT Article DE biomass; bole position; clones; correlation; feedstock quality; Populus; short rotation woody crops; tree growth; water stress; wood density ID WOOD DENSITY; POPULUS-DELTOIDES; GENETIC-VARIATION; BIOMASS; CLONES; PRODUCTIVITY; TRAITS; GROWTH; AGE; MANAGEMENT AB Specific gravity is an important consideration for traditional uses of hybrid poplars for pulp and solid wood products, as well as for biofuels and bioenergy production. While specific gravity has been shown to be under strong genetic control and subject to within-tree variability, the role of genotype x environment interactions is poorly understood. Most specific gravity reports are for a limited number of locations, resulting in a lack of information about the interactions between clones and sites over a wide range of climate and soil conditions. The objective of the current study was to characterize the effects of bole position, site, clone, and site x clone interactions for twelve hybrid poplar genotypes grown in Iowa, Minnesota, Wisconsin, and Michigan, USA. Observed specific gravities ranged from 0.267 to 0.495 (mean = 0.352 +/- 0.001 for 612 samples taken from 204 trees), with bole position and site x clone interactions having significant effects on specific gravity. Further investigation of the site x clone interactions indicated that environmental conditions related to water stress were key predictors of specific gravity. These data are important for informing genotypic selection and silvicultural management decisions associated with growing hybrid poplars. C1 [Headlee, William L.; Hall, Richard B.; Bauer, Edmund O.; Randall, Jesse A.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Zalesny, Ronald S., Jr.; Birr, Bruce A.; Wiese, Adam H.] US Forest Serv, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI 54501 USA. [Bender, Bradford; Miller, Raymond O.] Michigan State Univ, Forest Biomass Innovat Ctr, Dept Forestry, Escanaba, MI 49829 USA. RP Zalesny, RS (reprint author), US Forest Serv, No Res Stn, Inst Appl Ecosyst Studies, 5985 Highway K, Rhinelander, WI 54501 USA. EM wheadlee@iastate.edu; rzalesny@fs.fed.us; rbhall@iastate.edu; ebauer@charter.net; benderb@msu.edu; bbirr@fs.fed.us; rmiller@anr.msu.edu; randallj@iastate.edu; awiese@fs.fed.us FU U.S. Forest Service Research and Development Washington Office Woody Biomass, Bioenergy, and Bioproducts Program; U.S. Forest Service Northern Research Station Climate Change Science Council; Institute for Applied Ecosystem Studies [RWU-NRS-13] FX This study was funded by the U.S. Forest Service Research and Development Washington Office Woody Biomass, Bioenergy, and Bioproducts Program, as well as the U.S. Forest Service Northern Research Station Climate Change Science Council and the Institute for Applied Ecosystem Studies (RWU-NRS-13). We thank the following research collaborators for access to their field sites: Gregg Johnson (Waseca; University of Minnesota), Glen Stanosz (Arlington; University of Wisconsin), Jeff Strock (Lamberton; University of Minnesota), and Tim Wood (Lancaster; University of Wisconsin). In addition, we are grateful to the private landowners who let us harvest their trees on the remaining sites. We also thank Sue Lietz for creating Figure 1; Kricket Koehn for laboratory assistance; David Coyle and J. Y. Zhu for review of earlier versions of the manuscript. NR 45 TC 5 Z9 5 U1 0 U2 9 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2013 VL 4 IS 2 BP 251 EP 269 DI 10.3390/f4020251 PG 19 WC Forestry SC Forestry GA 169IH UT WOS:000320773200003 ER PT J AU Lee, J Mudge, KW AF Lee, Jinwook Mudge, Kenneth W. TI Gypsum effects on plant growth, nutrients, ginsenosides, and their relationship in American ginseng SO HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY LA English DT Article DE agroforestry systems; ginseng; EC; ginsenosides; HPLC ID PANAX-QUINQUEFOLIUS; SOIL CHEMISTRY; SODIC SOILS; YIELD; LIME; ROOT; PHOSPHORUS; LEAF; PERFORMANCE; ACIDITY AB Wild American ginseng is typically found in the shade of deciduous forests, in slightly acidic soils with a relatively high Ca content. Wood-cultivated ginseng is often grown using forest farming agroforestry systems under similar conditions. Supplementing Ca by soil incorporation of gypsum (CaSO4 center dot 2H(2)O) is often recommended for wood-cultivated ginseng. The objective of this study was to determine the effects of gypsum application on ginseng growth, tissue nutrients, and ginsenoside contents in American ginseng. Three-year-old rootlets were grown for 120 days with 0, 2, 4, 8, or 16 Mt center dot ha(-1) gypsum in greenhouse containers. Gypsum application reduced the soil pH slightly and elevated soil electrical conductivity (EC) and available soil Ca and S contents. While the Ca content in the ginseng increased with increasing levels of applied gypsum, shoot and root growth decreased. Root fresh weight prior to transplanting primarily affected the contents of ginsenoside Re, Rb1, Rc, and Rd and total ginsenosides. Gypsum treatment increased ginsenoside Rb1, Rc, and Rd and total ginsenoside contents. Furthermore, soil Ca, Mn, Cu, and Al contents positively correlated with total ginsenoside, but Mg was negatively correlated. HPLC analysis of root ginsenosides revealed that, although the concentrations of ginsenoside Rb1, Rc, and Rd increased with gypsum treatment, the contents of total ginsenosides were reduced. Changes in concentration may result from reduced root growth. Therefore, the results suggest that excess gypsum application (over 2 Mt center dot ha(-1)) is not beneficial for American ginseng production due to reduced plant growth. C1 [Lee, Jinwook; Mudge, Kenneth W.] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Lee, Jinwook] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Lee, J (reprint author), Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. EM jl425@cornell.edu FU Department of Horticulture graduate research assistantship FX We gratefully acknowledge the technical support of Joe Lardner and Dr. Wansang Lim, Jinwook Lee was supported by a Department of Horticulture graduate research assistantship. NR 34 TC 1 Z9 1 U1 3 U2 20 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 2211-3452 EI 2211-3460 J9 HORTIC ENVIRON BIOTE JI Hortic. Environ. Biotechnol. PD JUN PY 2013 VL 54 IS 3 BP 228 EP 235 DI 10.1007/s13580-013-0029-7 PG 8 WC Horticulture SC Agriculture GA 179KK UT WOS:000321519000005 ER PT J AU Havens, S Marshall, HP Pielmeier, C Elder, K AF Havens, Scott Marshall, Hans-Peter Pielmeier, Christine Elder, Kelly TI Automatic Grain Type Classification of Snow Micro Penetrometer Signals With Random Forests SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Classification algorithms; hydrology; remote sensing; snow ID STRATIGRAPHY AB Snow microstructure plays an important role in the remote sensing of snow water equivalent (SWE) for both passive and active microwave radars. The accuracy of microwave SWE retrieval algorithms is sensitive to (usually unknown) changes in microstructure. These algorithms could be improved with high-resolution estimates of microstructural properties by using an advanced instrument such as the Snow Micro Penetrometer (SMP), which measures penetration force at the millimeter scale and is sensitive to microstructure. The SMP can also take full micromechanical measurements at much greater speed and resolution and without observer bias than a traditional snow pit. Previous studies have shown that the snowpack stratigraphy and grain type can be accurately classified with one SMP measurement using basic statistics and classification trees (CTs). For this study, we used basic statistical measures of the penetration force and micromechanical estimates from an SMP inversion algorithm to significantly improve the classification accuracy of grain type and layer discrimination. We applied random forest (RF) techniques to classify three snow grain types (new snow, rounds, and facets) from SMP measurements collected in Switzerland and Grand Mesa, Colorado. RFs performed up to 8% better than single CTs, with overall misclassification errors between 17% and 40%. The coefficient of variation of the penetration force proved to be the most important variable, followed by variables that contain information about grain size like microscale strength and the number of ruptures. C1 [Havens, Scott; Marshall, Hans-Peter] Boise State Univ, Dept Geosci, Boise, ID 83725 USA. [Havens, Scott; Marshall, Hans-Peter] Boise State Univ, Ctr Geophys Invest Shallow Subsurface, Boise, ID 83725 USA. [Pielmeier, Christine] WSL, Inst Snow & Avalanche Res SLF, CH-7260 Davos, Switzerland. [Elder, Kelly] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. RP Havens, S (reprint author), Boise State Univ, Dept Geosci, Boise, ID 83725 USA. EM scotthavens@u.boisestate.edu; hpmarshall@boisestate.edu; pielmeier@slf.ch; kelder@fs.fed.us FU National Aeronautics and Space Administration (NASA) Earth and Space Systems Science Graduate Fellowship [NNX10AP46H]; NASA Grand Mesa Ground-Based Calibration [NNX10AU61G]; NASA Remote Sensing of the Cryosphere [NNX10AP46H] FX This work was supported in part by the National Aeronautics and Space Administration (NASA) Earth and Space Systems Science Graduate Fellowship under Grant NNX10AP46H, by NASA Grand Mesa Ground-Based Calibration under Grant NNX10AU61G, and by NASA Remote Sensing of the Cryosphere under Grant NNX10AP46H. NR 31 TC 6 Z9 6 U1 1 U2 22 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD JUN PY 2013 VL 51 IS 6 BP 3328 EP 3335 DI 10.1109/TGRS.2012.2220549 PN 1 PG 8 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 171PE UT WOS:000320940600011 ER PT J AU Wyatt, VT Yadav, MP Latona, N Liu, CK AF Wyatt, Victor T. Yadav, Madhav P. Latona, Nicholas Liu, Cheng-Kung TI Thermal and Mechanical Properties of Glycerol-Based Polymer Films Infused with Plant Cell Wall Polysaccharidese SO JOURNAL OF BIOBASED MATERIALS AND BIOENERGY LA English DT Article DE Glycerol; Films; Thermal Properties; Mechanical Properties ID POLY(LACTIC ACID); COMPOSITE FILMS; FIBER; POLYCARDANOL; HYDROGELS; DELIVERY; BLENDS AB Poly(glutaric acid-co-glycerol) films were produced by first synthesizing polymer gels from the uncatalyzed polyesterification of glutaric acid and glycerol in toluene. Residual amounts of starting materials in the gel matrices were determined by gas chromatography (GC) to contain 16.4% glutaric acid and 0.2% glycerol or 0.6% glutaric acid and 15% glycerol, respectively for the 2:1 and 1:2 (glutaric acid:glycerol) molar ratio formulations. The 2:1 (glutaric acid:glycerol) polymer gel formulation was cured with and without the incorporation of lignocellulosic biomass (sugarcane bagasse) or purified plant cell wall polysaccharides (corn fiber gum, pectin, or microcrystalline cellulose) at 135 degrees C for 12 h to form polymer composite films. The 1:2 (glutaric acid:glycerol) gel formulation was cured at 135 C for 24 h. ATR-IR results suggest that the films were completely cured. Heat capacity (Delta C-p) and glass transition temperature (T-g) for all specimens averaged 0.41 (+/- 0.1) J/g*degrees C and -14.8 (+/- 2.0)degrees C, respectively. The thermal curves of the polymers showed total degradation for all polymer films at approximately 400 degrees C. The addition of nnicrocrystalline cellulose into the polymer matrix showed a 3-fold increase in tensile strength and Young's modulus and a two-fold increase in fracture energy when compared to the cellulose-free polymer film made only from a 2:1 molar ratio of glutaric acid to glycerol. C1 [Wyatt, Victor T.; Yadav, Madhav P.; Latona, Nicholas; Liu, Cheng-Kung] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Wyatt, VT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM victor.wyatt@ars.usda.gov NR 37 TC 3 Z9 3 U1 0 U2 11 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1556-6560 J9 J BIOBASED MATER BIO JI J. Biobased Mater. Bioenergy PD JUN PY 2013 VL 7 IS 3 BP 348 EP 356 DI 10.1166/jbmb.2013.1368 PG 9 WC Chemistry, Applied; Energy & Fuels; Materials Science, Biomaterials SC Chemistry; Energy & Fuels; Materials Science GA 180MY UT WOS:000321600500006 ER PT J AU Saha, BC Nichols, NN Cotta, MA AF Saha, Badal C. Nichols, Nancy N. Cotta, Michael A. TI Comparison of Separate Hydrolysis and Fermentation versus Simultaneous Saccharification and Fermentation of Pretreated Wheat Straw to Ethanol by Saccharomyces cerevisiae SO JOURNAL OF BIOBASED MATERIALS AND BIOENERGY LA English DT Article DE Ethanol; Saccharomyces cerevisiae; Separate Hydrolysis and Fermentation; Simultaneous Saccharification and Fermentation; Wheat Straw Hydrolyzate; Biodetoxification of Fermentation Inhibitors ID ESCHERICHIA-COLI; ENZYMATIC-HYDROLYSIS; DETOXIFICATION; CA(OH)(2); BIOMASS; FBR5 AB Ethanol production by Saccharomyces cerevisiae NRRL Y-2034 from wheat straw (WS) by separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) was studied. The yield of glucose from dilute acid pretreated WS (86 g/liter) after enzymatic saccharification was 26.9 +/- 0.1 g/liter. The pretreated WS was biologically conditioned to remove inhibitors by growing Coniochaeta ligniaria NRRL 30616 aerobically in the liquid portion for 15 h. The yeast produced 13.2 +/- 0.2 g ethanol per liter in 94 h from non-detoxified WS hydrolyzate (WSH) at pH 5.5 and 37 degrees C. It produced 13.3 +/- 0.0 g ethanol per liter in 30 h from the biodetoxified WSH by SHF. In SSF experiments, the strain produced 13.0 +/- 0.7 g ethanol per liter in 72 h from the non-detoxified WS but took 48 h to produce 13.1 +/- 0.1 g ethanol per liter from biodetoxified WS. By comparison, SSF of washed solid residues yielded 12.6 +/- 0.4 g ethanol per liter in 43 h. SSF offered advantage over SHF in reducing the total time of conversion of pretreated WS to ethanol by 53-57%. C1 [Saha, Badal C.; Nichols, Nancy N.; Cotta, Michael A.] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Saha, BC (reprint author), USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Badal.Saha@ars.usda.gov NR 23 TC 6 Z9 6 U1 2 U2 11 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1556-6560 J9 J BIOBASED MATER BIO JI J. Biobased Mater. Bioenergy PD JUN PY 2013 VL 7 IS 3 BP 409 EP 414 DI 10.1166/jbmb.2013.1366 PG 6 WC Chemistry, Applied; Energy & Fuels; Materials Science, Biomaterials SC Chemistry; Energy & Fuels; Materials Science GA 180MY UT WOS:000321600500012 ER PT J AU Cromartie, J Nulph, D Hart, G Dobis, E AF Cromartie, John Nulph, David Hart, Gary Dobis, Elizabeth TI Defining frontier areas in the United States SO JOURNAL OF MAPS LA English DT Article ID NONMETROPOLITAN GREAT-PLAINS; AGGLOMERATION ECONOMIES; POPULATION AB Demand is growing for a statistically based, nationally consistent definition of frontier territory, one that is adjustable within a reasonable range and applicable in different research and policy contexts. The need arises from Congressional mandates affecting rural health programs and from limitations of previous classification schemes. As used here, the term frontier denotes territory characterized by some combination of relatively low-population density and high geographic remoteness. Two features distinguish the methodology described here from earlier classifications. First, the approach strives for the most accurate measures of distance possible for the smallest units of geography containing population data. Travel time by car to nearby urban areas is calculated for coterminous US territory at the 1x1 kilometer grid level. Once frontier territory is delimited at the grid level, frontier populations may be summed to ZIP code areas, as demonstrated on the Main Map, or to census tracts, counties, or other useful geographic entities. Second, travel-time thresholds around urban areas were allowed to vary by urban-area population size. This is desirable because the effect of urban population size on adjacent rural population density is discontinuous. At any given distance from an urban area, population density increases as the size of the nearby urban area increases. C1 [Cromartie, John; Nulph, David] USDA, Econ Res Serv, Washington, DC 20250 USA. [Hart, Gary] Univ N Dakota, Sch Med & Hlth Sci, Ctr Rural Hlth, Ctr Rural Med, Grand Forks, ND 58201 USA. [Dobis, Elizabeth] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. RP Cromartie, J (reprint author), USDA, Econ Res Serv, Washington, DC 20250 USA. EM jbc@ers.usda.gov FU Economic Research Service, US Department of Agriculture; Federal Office of Rural Health Policy, US Department of Health and Human Services FX This research was funded by the Economic Research Service, US Department of Agriculture and the Federal Office of Rural Health Policy, US Department of Health and Human Services. The authors wish to thank the many local, state, and regional experts who provided valuable input at four frontier workshops held prior to undertaking this project. The authors also acknowledge the valuable insights from Andrew Isserman, Vince Breneman, Eric Larson, Richard Morrill, David Plane, and Michael Ratcliffe. NR 26 TC 0 Z9 0 U1 1 U2 10 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1744-5647 J9 J MAPS JI J. Maps PD JUN 1 PY 2013 VL 9 IS 2 BP 149 EP 153 DI 10.1080/17445647.2013.773569 PG 5 WC Geography; Geography, Physical SC Geography; Physical Geography GA 176MM UT WOS:000321308800002 ER PT J AU Krishnamurthy, P Singh, RJ Tsukamoto, C Park, JH Lee, JD Chung, G AF Krishnamurthy, Panneerselvam Singh, Ram J. Tsukamoto, Chigen Park, Jong Hyun Lee, Jeong Dong Chung, Gyuhwa TI Kunitz trypsin inhibitor polymorphism in the Korean wild soybean (Glycine soja Sieb. & Zucc.) SO PLANT BREEDING LA English DT Article DE Kunitz trypsin inhibitor; wild soybean; germplasm; protease inhibitors; geographical distribution ID PROTEIN ELECTROPHORESIS PROFILES; SEED PROTEIN; DISC ELECTROPHORESIS; GENES; MAX; DISSEMINATION; TOBACCO; VARIANT; PLANTS; TIB AB Kunitz trypsin inhibitor (KTi) in 1368 accessions of wild soybean (Glycine soja Sieb. & Zucc.), collected from three regions of Korea, was examined for allelic diversity and geographical distribution. Five electrophoretically distinguishable KTi forms were detected: three were common (Tia, Tib and Tia/Tib) and two were previously unreported (Tibi7-1 and Tibi5). The Tia allele was predominant (93.49%). Alleles Tib, Tibi7-1 and Tibi5 were detected with the frequencies of 3.47, 0.55 and 0.11%, respectively. The heterozygous form (Tia/Tib) was detected with the frequency of 2.26%. The nucleotide sequence of Tibi7-1 was identical to that of the Tib-derived variant allele Tif, with the exception of three nucleotides: AG at position +244, AC at position +286 and GC at position +601. The latter two were similar to Tia, suggesting that Tibi7-1 is an intermediate allele between Tia and Tib. The gene for Tibi5 showed 100% similarity with the Japanese intermediate allele Tibi5. This study demonstrates that Korean wild soybeans are remarkably rich source of new KTi alleles not reported before. C1 [Krishnamurthy, Panneerselvam; Chung, Gyuhwa] Chonnam Natl Univ, Dept Biotechnol, Yeosu 550749, Chonnam, South Korea. [Singh, Ram J.] Univ Illinois, USDA ARS, Natl Soybean Res Lab, Urbana, IL 61801 USA. [Tsukamoto, Chigen] Iwate Univ, Dept Appl Biol Chem, Morioka, Iwate 0208550, Japan. [Park, Jong Hyun] RDA, Natl Agrodivers Ctr, NAAS, Suwon 441853, Gyeonggi, South Korea. [Lee, Jeong Dong] Kyungpook Natl Univ, Div Plant Biosci, Taegu 702701, South Korea. RP Chung, G (reprint author), Chonnam Natl Univ, Dept Biotechnol, Yeosu 550749, Chonnam, South Korea. EM chung@chonnam.ac.kr OI Chung, Gyuhwa/0000-0001-8053-689X FU Chonnam National University FX We thank Dr. Sang Mi Sun for maintaining the germplasm collections. This study was financially supported by Chonnam National University, 2011. NR 30 TC 1 Z9 1 U1 2 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2013 VL 132 IS 3 BP 311 EP 316 DI 10.1111/pbr.12058 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 160KG UT WOS:000320117800012 ER PT J AU Urban, JF Hu, Y Miller, MM Scheib, U Yiu, YY Aroian, RV AF Urban, Joseph F., Jr. Hu, Yan Miller, Melanie M. Scheib, Ulrike Yiu, Ying Y. Aroian, Raffi V. TI Bacillus thuringiensis-derived Cry5B Has Potent Anthelmintic Activity against Ascaris suum SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Article ID CAENORHABDITIS-ELEGANS; NEMATODE INFECTIONS; TOXIN RESISTANCE; BACTERIAL TOXIN; FEED ADDITIVES; ALBENDAZOLE; PARASITES; CHILDREN; EFFICACY; PROTEIN AB Ascaris suum and Ascaris lumbricoides are two closely related geo-helminth parasites that ubiquitously infect pigs and humans, respectively. Ascaris suum infection in pigs is considered a good model for A. lumbricoides infection in humans because of a similar biology and tissue migration to the intestines. Ascaris lumbricoides infections in children are associated with malnutrition, growth and cognitive stunting, immune defects, and, in extreme cases, life-threatening blockage of the digestive tract and aberrant migration into the bile duct and peritoneum. Similar effects can be seen with A. suum infections in pigs related to poor feed efficiency and performance. New strategies to control Ascaris infections are needed largely due to reduced treatment efficacies of current anthelmintics in the field, the threat of resistance development, and the general lack of new drug development for intestinal soil-transmitted helminths for humans and animals. Here we demonstrate for the first time that A. suum expresses the receptors for Bacillus thuringiensis crystal protein and novel anthelmintic Cry5B, which has been previously shown to intoxicate hookworms and which belongs to a class of proteins considered non-toxic to vertebrates. Cry5B is able to intoxicate A. suum larvae and adults and triggers the activation of the p38 mitogen-activated protein kinase pathway similar to that observed with other nematodes. Most importantly, two moderate doses of 20 mg/kg body weight (143 nM/kg) of Cry5B resulted in a near complete cure of intestinal A. suum infections in pigs. Taken together, these results demonstrate the excellent potential of Cry5B to treat Ascaris infections in pigs and in humans and for Cry5B to work effectively in the human gastrointestinal tract. C1 [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Hu, Yan; Miller, Melanie M.; Scheib, Ulrike; Yiu, Ying Y.; Aroian, Raffi V.] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. RP Urban, JF (reprint author), ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. EM raroian@ucsd.edu OI Urban, Joseph/0000-0002-1590-8869 FU National Institutes of Health/National Institute of Allergy and Infectious [2R01AI056189]; USDA/ARS [1265-32000-084] FX This work was funded by National Institutes of Health/National Institute of Allergy and Infectious grant 2R01AI056189 to RVA and by funds from USDA/ARS project number 1265-32000-084 to JFU. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 12 Z9 12 U1 0 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD JUN PY 2013 VL 7 IS 6 AR e2263 DI 10.1371/journal.pntd.0002263 PG 7 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA 175AL UT WOS:000321201300024 PM 23818995 ER PT J AU Wiemann, P Sieber, CMK Von Bargen, KW Studt, L Niehaus, EM Espino, JJ Huss, K Michielse, CB Albermann, S Wagner, D Bergner, SV Connolly, LR Fischer, A Reuter, G Kleigrewe, K Bald, T Wingfield, BD Ophir, R Freeman, S Hippler, M Smith, KM Brown, DW Proctor, RH Munsterkotter, M Freitag, M Humpf, HU Guldener, U Tudzynski, B AF Wiemann, Philipp Sieber, Christian M. K. Von Bargen, Katharina W. Studt, Lena Niehaus, Eva-Maria Espino, Jose J. Huss, Kathleen Michielse, Caroline B. Albermann, Sabine Wagner, Dominik Bergner, Sonja V. Connolly, Lanelle R. Fischer, Andreas Reuter, Gunter Kleigrewe, Karin Bald, Till Wingfield, Brenda D. Ophir, Ron Freeman, Stanley Hippler, Michael Smith, Kristina M. Brown, Daren W. Proctor, Robert H. Munsterkotter, Martin Freitag, Michael Humpf, Hans-Ulrich Guldener, Ulrich Tudzynski, Bettina TI Deciphering the Cryptic Genome: Genome-wide Analyses of the Rice Pathogen Fusarium fujikuroi Reveal Complex Regulation of Secondary Metabolism and Novel Metabolites SO PLOS PATHOGENS LA English DT Article ID GIBBERELLIN BIOSYNTHETIC-PATHWAY; MANGO MALFORMATION DISEASE; POLYKETIDE SYNTHASE GENES; RED PIGMENT BIKAVERIN; ASPERGILLUS-NIDULANS; FUMONISIN PRODUCTION; NEUROSPORA-CRASSA; MASS-SPECTROMETRY; SPECIES COMPLEX; FUNCTIONAL-CHARACTERIZATION AB The fungus Fusarium fujikuroi causes "bakanae" disease of rice due to its ability to produce gibberellins (GAs), but it is also known for producing harmful mycotoxins. However, the genetic capacity for the whole arsenal of natural compounds and their role in the fungus' interaction with rice remained unknown. Here, we present a high-quality genome sequence of F. fujikuroi that was assembled into 12 scaffolds corresponding to the 12 chromosomes described for the fungus. We used the genome sequence along with ChIP-seq, transcriptome, proteome, and HPLC-FTMS-based metabolome analyses to identify the potential secondary metabolite biosynthetic gene clusters and to examine their regulation in response to nitrogen availability and plant signals. The results indicate that expression of most but not all gene clusters correlate with proteome and ChIP-seq data. Comparison of the F. fujikuroi genome to those of six other fusaria revealed that only a small number of gene clusters are conserved among these species, thus providing new insights into the divergence of secondary metabolism in the genus Fusarium. Noteworthy, GA biosynthetic genes are present in some related species, but GA biosynthesis is limited to F. fujikuroi, suggesting that this provides a selective advantage during infection of the preferred host plant rice. Among the genome sequences analyzed, one cluster that includes a polyketide synthase gene (PKS19) and another that includes a non-ribosomal peptide synthetase gene (NRPS31) are unique to F. fujikuroi. The metabolites derived from these clusters were identified by HPLC-FTMS-based analyses of engineered F. fujikuroi strains overexpressing cluster genes. In planta expression studies suggest a specific role for the PKS19-derived product during rice infection. Thus, our results indicate that combined comparative genomics and genome-wide experimental analyses identified novel genes and secondary metabolites that contribute to the evolutionary success of F. fujikuroi as a rice pathogen. C1 [Wiemann, Philipp; Niehaus, Eva-Maria; Espino, Jose J.; Huss, Kathleen; Michielse, Caroline B.; Albermann, Sabine; Wagner, Dominik; Tudzynski, Bettina] Univ Munster, Inst Biol & Biotechnol Pflanzen Mol Biol & Biotec, D-48149 Munster, Germany. [Sieber, Christian M. K.; Guldener, Ulrich] German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Munchen, Inst Bioinformat & Syst Biol, Neuherberg, Germany. [Von Bargen, Katharina W.; Studt, Lena; Kleigrewe, Karin; Humpf, Hans-Ulrich] Univ Munster, Inst Food Chem, D-48149 Munster, Germany. [Bergner, Sonja V.; Bald, Till; Freitag, Michael] Univ Munster, Inst Biol & Biotechnol Pflanzen Plant Biochem, D-48149 Munster, Germany. [Connolly, Lanelle R.; Smith, Kristina M.; Freitag, Michael] Oregon State Univ, Ctr Genome Res & Biocomputing, Dept Biochem & Biophys, Corvallis, OR 97331 USA. [Fischer, Andreas; Reuter, Gunter] Univ Halle Wittenberg, Inst Genet Dev Genet, D-06108 Halle, Germany. [Wingfield, Brenda D.] Univ Pretoria, Dept Genet, ZA-0002 Pretoria, South Africa. [Ophir, Ron] Agr Res Org, Volcani Ctr, Inst Plant Sci, IL-50250 Bet Dagan, Israel. [Freeman, Stanley] Agr Res Org, Volcani Ctr, Dept Plant Pathol, IL-50250 Bet Dagan, Israel. [Brown, Daren W.; Proctor, Robert H.] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. RP Wiemann, P (reprint author), Univ Munster, Inst Biol & Biotechnol Pflanzen Mol Biol & Biotec, D-48149 Munster, Germany. EM humpf@uni-muenster.de; u.gueldener@helmholtz-muenchen.de; tudzynsb@uni-muenster.de RI Wingfield, Brenda/B-1376-2008; Guldener, Ulrich/G-5227-2012; OI Wingfield, Brenda/0000-0002-6189-1519; Guldener, Ulrich/0000-0001-5052-8610; Wiemann, Philipp/0000-0002-7983-2218; Sieber, Christian/0000-0002-9560-0281; Bald, Till/0000-0001-5341-0079 FU Deutsche Forschungsgesellschaft [DFG TU 101/16, HU 730/9, GU 1205/1, GU 1205/2]; NIH [GM097637]; ACS [RSG-08-030-01-CCG]; Austrian Science Fund FWF [F3705] FX This work was supported by funds of the Deutsche Forschungsgesellschaft (DFG TU 101/16; HU 730/9; GU 1205/1, GU 1205/2) and by grants from the NIH (GM097637) and ACS (RSG-08-030-01-CCG) to MF. UG was funded by the Austrian Science Fund FWF (special research project Fusarium, F3705). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 162 TC 101 Z9 111 U1 12 U2 132 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD JUN PY 2013 VL 9 IS 6 AR e1003475 DI 10.1371/journal.ppat.1003475 PG 35 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA 175CG UT WOS:000321206600062 PM 23825955 ER PT J AU Rothrock, MJ Szogi, AA Vanotti, MB AF Rothrock, M. J., Jr. Szoegi, A. A. Vanotti, M. B. TI Recovery of ammonia from poultry litter using flat gas permeable membranes SO WASTE MANAGEMENT LA English DT Article DE Poultry waste; Animal waste treatment; Nitrogen recovery; Ammonia capture; Ammonia emissions; Gas permeable membrane ID LIVESTOCK OPERATIONS; HOUSES; EMISSIONS; NITROGEN; WORKERS AB The use of flat gas-permeable membranes was investigated as components of a new process to capture and recover ammonia (NH3) in poultry houses. This process includes the passage of gaseous NH3 through a microporous hydrophobic membrane, capture with a circulating dilute acid on the other side of the membrane, and production of a concentrated ammonium (NH4) salt. Bench- and pilot-scale prototype systems using flat expanded polytetrafluoroethylene (ePTFE) membranes and a sulfuric acid solution consistently reduced headspace NH3 concentrations from 70% to 97% and recovered 88% to 100% of the NH3 volatilized from poultry litter. The potential benefits of this technology include cleaner air inside poultry houses, reduced ventilation costs, and a concentrated liquid ammonium salt that can be used as a plant nutrient solution. Published by Elsevier Ltd. C1 [Rothrock, M. J., Jr.; Szoegi, A. A.; Vanotti, M. B.] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Rothrock, M. J., Jr.] USDA ARS, Poultry Proc & Swine Physiol Res Unit, Athens, GA 30605 USA. RP Szogi, AA (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas St, Florence, SC 29501 USA. EM ariel.szogi@ars.usda.gov FU ARS Post-Doctoral Research Associate Program [RA-08-012H]; USDA-ARS National Program 214: Agriculture and Industrial Byproduct Utilization: ARS Project [6657-13630-005-00D] FX This research was part of USDA-ARS National Program 214: Agriculture and Industrial Byproduct Utilization: ARS Project 6657-13630-005-00D "Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization." The senior author was supported by a grant from the ARS Post-Doctoral Research Associate Program (#RA-08-012H). The authors would like to thank Laura Smith, William Brigman, and Louis Hill for their assistance in enclosure fabrication, litter sample preparation, and sample analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 32 TC 6 Z9 6 U1 2 U2 34 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0956-053X J9 WASTE MANAGE JI Waste Manage. PD JUN PY 2013 VL 33 IS 6 BP 1531 EP 1538 DI 10.1016/j.wasman.2013.03.011 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 177VK UT WOS:000321403300026 PM 23571072 ER PT J AU Liang, KM Zhang, JE Fang, L Zhao, BL Luo, MZ Parajuli, P Ouyang, Y AF Liang, Kaiming Zhang, Jia-en Fang, Li Zhao, Benliang Luo, Mingzhu Parajuli, Prem Ouyang, Ying TI The biological control of Pomacea canaliculata population by rice-duck mutualism in paddy fields SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Pomacea canaliculata Lamarck; rice-duck mutualism system; biological control; population dynamics; trait-mediated indirect interactions; density-mediated indirect interactions ID GOLDEN APPLE SNAIL; MEDIATED INDIRECT INTERACTIONS; PREDATION RISK; ECOLOGICAL COMMUNITIES; MATING-BEHAVIOR; ASIAN WETLANDS; WATER STRIDER; MATE CHOICE; FISH; ECOSYSTEM AB Duck has been used as a non-chemical control method against Pomacea canaliculata Lamarck, but little is known about its principles that underlie the control of snail populations. An indoor experiment was initially used to observe the predation potential of ducks, followed by replicated field trials. In the indoor studies, ducks effectively preyed on juvenile snails, but had a weak predatory effect on large snails and egg clusters. In the field, application of a rice-duck mutualism system significantly reduced the numbers of snails (especially number of immature individuals), number of snail egg clusters and snail damage to rice plants. The controlling effect was longer and more stable than the chemical application, resulting in a better yield than with the pentachlorophenol sodium and tea seed powder treatment. Our experimental results also suggested that the snail age structure in the rice-duck mutualism plots was shifted towards older snails by ducks preying, indicating a trend towards population decline, and ducks caused snails to oviposit on sites not ideal for hatchling establishment. Throughout the studies, it is suggested that a rice-duck mutualism system could be used for controlling P. canaliculata in organic rice production. C1 [Liang, Kaiming; Zhang, Jia-en; Fang, Li; Zhao, Benliang; Luo, Mingzhu] South China Agr Univ, Dept Ecol, Coll Agr, Guangzhou, Guangdong, Peoples R China. [Zhang, Jia-en; Zhao, Benliang] South China Agr Univ, Minist Agr Peoples Republ China, Key Lab Ecol Agr, Guangzhou, Guangdong, Peoples R China. [Parajuli, Prem] Mississippi State Univ, Dept Agr & Biol Engn, Starkville, MS USA. [Ouyang, Ying] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Thompson Hall, MS USA. RP Zhang, JE (reprint author), South China Agr Univ, Dept Ecol, Coll Agr, Guangzhou, Guangdong, Peoples R China. EM jeanzh@scau.edu.cn FU National Basic Research Program of China [2006CB100206, 2011CB100406]; National Natural Science Foundation of China [U1131006, 30770403, 30900187]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20114404120007] FX This work was supported by the National Basic Research Program of China (No. 2006CB100206, No. 2011CB100406), the National Natural Science Foundation of China (No. U1131006, No. 30770403, No. 30900187) and the Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 20114404120007). NR 69 TC 2 Z9 4 U1 7 U2 45 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUN 1 PY 2013 VL 23 IS 6 BP 674 EP 690 DI 10.1080/09583157.2013.790933 PG 17 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 168GA UT WOS:000320692500006 ER PT J AU Liebhold, AM Plymale, R Elkinton, JS Hajek, AE AF Liebhold, Andrew M. Plymale, Ruth Elkinton, Joseph S. Hajek, Ann E. TI Emergent fungal entomopathogen does not alter density dependence in a viral competitor SO ECOLOGY LA English DT Article DE competition; density dependence; Entomophaga maimaiga; gypsy moth; host-pathogen dynamics; Lepidoptera; Lymantria dispar; nucleopolyhedrovirus; pathogens ID GYPSY-MOTH POPULATIONS; NUCLEAR POLYHEDROSIS-VIRUS; WITHIN-HOST INTERACTIONS; ENTOMOPHAGA-MAIMAIGA; LEPIDOPTERA; PATHOGENS; ENTOMOPHTHORALES; TRANSMISSION; MORTALITY; DISEASES AB Population cycles in forest Lepidoptera often result from recurring density-dependent epizootics of entomopathogens. While these systems are typically dominated by a single pathogen species, insects are often infected by multiple pathogens, yet little is known how pathogens interact to affect host dynamics. The apparent invasion of northeastern North America by the fungal entomopathogen Entomophaga maimaiga some time prior to 1989 provides a unique opportunity to evaluate such interactions. Prior to the arrival of E. maimaga, the oscillatory dynamics of host gypsy moth, Lymantria dispar, populations were apparently driven by epizootics of a nucleopolyhedrovirus. Subsequent to its emergence, E. maimaiga has caused extensive mortality in host populations, but little is known about how it has altered multigenerational dynamics of the gypsy moth and its virus. Here we compared demographic data collected in gypsy moth populations prior to vs. after E. maimaiga's invasion. We found that the recently invading fungal pathogen virtually always causes greater levels of mortality in hosts than does the virus, but fungal mortality is largely density independent. Moreover, the presence of the fungus has apparently not altered the gypsy moth-virus density-dependent interactions that were shown to drive periodic oscillations in hosts before the arrival of the fungus. C1 [Liebhold, Andrew M.] US Forest Serv, No Res Stn, Morgantown, WV 26505 USA. [Plymale, Ruth; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Elkinton, Joseph S.] Univ Massachusetts, Dept Plant Soil & Insect Sci, Amherst, MA 01003 USA. RP Liebhold, AM (reprint author), US Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. EM aliebhold@fs.fed.us RI Liebhold, Andrew/C-1423-2008 OI Liebhold, Andrew/0000-0001-7427-6534 FU USDA, CSREES [NRI 2006-1774]; USDA, Forest Service Forest Health Technology Enterprise Team FX B. Reed, M. Beck, and R. Annis provided excellent assistance in the field in Pennsylvania. B. Reed, J. Greenberg, K. Ciccaglione, J. Tyvoll, A. Navarro, T. James, M. Grambor, M. Garvey, C. Fritzen, A. Staron, K. Levine, J. Lee, M. Cunningham, A. Saylor, J. Hannam, and S. Finkbeiner assisted with diagnosis of pathogens and parasitoids. E. Luzader, G. Racin, A. Orozumbekov, P. Tobin, B. Reed, J. Greenberg, J. Hannam, and J. Liebherr helped count egg masses. We thank G. Felton, K. Hoover, O. Bjornstad, and other personnel with the Penn State Entomology Department for generously providing laboratory space and equipment. John Stanovick provided statistical advice. The research was funded by USDA, CSREES, NRI 2006-1774, and the USDA, Forest Service Forest Health Technology Enterprise Team. NR 33 TC 10 Z9 11 U1 3 U2 26 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD JUN PY 2013 VL 94 IS 6 BP 1217 EP 1222 PG 6 WC Ecology SC Environmental Sciences & Ecology GA 168OG UT WOS:000320714800002 PM 23923480 ER PT J AU Doddapaneni, H Subramanian, V Fu, BL Cullen, D AF Doddapaneni, Harshavardhan Subramanian, Venkataramanan Fu, Bolei Cullen, Dan TI A comparative genomic analysis of the oxidative enzymes potentially involved in lignin degradation by Agaricus bisporus SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Agaricus bisporus; Oxidase; Peroxidase; Cytochrome P450 monooxygenase; Comparative genomics; Litter-rot fungi ID FUNGUS PHANEROCHAETE-CHRYSOSPORIUM; BASIDIOMYCETE LENTINULA-EDODES; 2 LACCASE GENES; CULTIVATED MUSHROOM; COPRINUS-CINEREUS; GLYOXAL OXIDASE; MOLECULAR CHARACTERIZATION; FUNCTIONAL DIVERSITY; RHODOTORULA-MINUTA; BUTTON MUSHROOM AB The oxidative enzymatic machinery for degradation of organic substrates in Agaricus bisporus (Ab) is at the core of the carbon recycling mechanisms in this fungus. To date, 156 genes have been tentatively identified as part of this oxidative enzymatic machinery, which includes 26 peroxidase encoding genes, nine copper radical oxidase [including three putative glyoxal oxidase-encoding genes (GLXs)], 12 laccases sensu stricto and 109 cytochrome P450 monooxygenases. Comparative analyses of these enzymes in Ab with those of the white-rot fungus, Phanerochaete chrysosporium, the brown-rot fungus, Postia placenta, the coprophilic litter fungus, Coprinopsis cinerea and the ectomychorizal fungus, Laccaria bicolor, revealed enzyme diversity consistent with adaptation to substrates rich in humic substances and partially degraded plant material. For instance, relative to wood decay fungi, Ab cytochrome P450 genes were less numerous (109 gene models), distributed among distinctive families, and lacked extensive duplication and clustering. Viewed together with P450 transcript accumulation patterns in three tested growth conditions, these observations were consistent with the unique Ab lifestyle. Based on tandem gene arrangements, a certain degree of gene duplication seems to have occurred in this fungus in the copper radical oxidase (CRO) and the laccase gene families. In Ab, high transcript levels and regulation of the heme-thiolate peroxidases, two manganese peroxidases and the three GLX-like genes are likely in response to complex natural substrates, including lignocellulose and its derivatives, thereby suggesting an important role in lignin degradation. On the other hand, the expression patterns of the related CROs suggest a developmental role in this fungus. Based on these observations, a brief comparative genomic overview of the Ab oxidative enzyme machinery is presented. (C) 2013 Elsevier Inc. All rights reserved. C1 [Doddapaneni, Harshavardhan; Fu, Bolei] Univ Iowa, Dept Biol, Carver Ctr Genom, Iowa City, IA 52242 USA. [Subramanian, Venkataramanan] Colorado Sch Mines, Dept Chem & Geochem, Golden, CO 80401 USA. [Cullen, Dan] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Doddapaneni, H (reprint author), Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA. NR 75 TC 10 Z9 12 U1 5 U2 71 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUN PY 2013 VL 55 BP 22 EP 31 DI 10.1016/j.fgb.2013.03.004 PG 10 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 172TV UT WOS:000321028700004 PM 23583597 ER PT J AU Rowland, LJ Ogden, EL Takeda, F Glenn, DM Ehlenfeldt, MK Vinyard, BT AF Rowland, Lisa J. Ogden, Elizabeth L. Takeda, Fumiomi Glenn, David Michael Ehlenfeldt, Mark K. Vinyard, Bryan T. TI Variation among Highbush Blueberry Cultivars for Frost Tolerance of Open Flowers SO HORTSCIENCE LA English DT Article DE bud development; cold hardiness; freezing injury; freezing tolerance; Vaccinium corymbosum ID COLD-HARDINESS; BUD DEVELOPMENT; VACCINIUM-ASHEI; DEACCLIMATION; TEMPERATURES; GENOTYPES; ACCLIMATION; KINETICS; DAMAGE AB Injury of open flowers often occurs in fruit crops by late winter or early spring frosts and can result in significant reduction in yield. In this study, freezing tolerance of open flowers of five highbush blueberry cultivars, Bluecrop, Elliott, Hannah's Choice, Murphy, and Weymouth, was determined using two freezing methods. Methods involved either placing whole plants in a radiation frost chamber or detached shoots in a glycol-freezing bath. In both methods, plants (or excised shoots) with opening flowers were exposed to temperatures ranging from -2 to -10 degrees C. After freeze treatments, several flower parts were evaluated for damage and the lethal temperature(50) (LT50) determined. In order, from the most sensitive flower part to the least sensitive on average, were the corolla, filament, anther, style, exterior ovary, stigma, ovules, interior ovary, and placenta. A two-way analysis of variance (ANOVA) found no significant effect of the freezing method on the calculated freeze damage to most of the various flower parts. However, a significant genotype effect was found on freeze damage to the style, filament, anthers, and exterior ovary. Overall, 'Bluecrop' was the most sensitive to freezing, whereas 'Hannah's Choice' and 'Murphy' were the most freezing-tolerant. In conclusion, genotypic variability in frost tolerance of open highbush blueberry flowers was detected, which can be exploited in breeding for more frost-tolerant cultivars. C1 [Rowland, Lisa J.; Ogden, Elizabeth L.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Takeda, Fumiomi; Glenn, David Michael] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Ehlenfeldt, Mark K.] ARS, USDA, Genet Improvement Fruits & Vegetables Lab, Marucci Ctr Blueberry & Cranberry Res & Extens, Chatsworth, NJ 08019 USA. [Vinyard, Bryan T.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Biometr Consulting Serv, Beltsville, MD 20705 USA. RP Rowland, LJ (reprint author), ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Genet Improvement Fruits & Vegetables Lab, Bldg 010A,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM jeannine.rowland@ars.usda.gov NR 23 TC 5 Z9 6 U1 2 U2 20 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD JUN PY 2013 VL 48 IS 6 BP 692 EP 695 PG 4 WC Horticulture SC Agriculture GA 168BU UT WOS:000320681500003 ER PT J AU Lafta, A Mou, BQ AF Lafta, Abbas Mou, Beiquan TI Evaluation of Lettuce Genotypes for Seed Thermotolerance SO HORTSCIENCE LA English DT Article DE Lactuca sativa; thermoinhibition; thermodormancy; crisphead lettuce; butterhead lettuce; romaine lettuce ID ABSCISIC-ACID; MATURATION TEMPERATURE; CRISPHEAD LETTUCE; LACTUCA-SATIVA; CLIMATE-CHANGE; HEAT-STRESS; GERMINATION; THERMOINHIBITION; ETHYLENE; DORMANCY AB Thermoinhibition of lettuce (Lactuca sativa L.) seed germination is a common problem associated with lettuce production. Depending on lettuce cultivars, seed germination may be inhibited when temperatures exceed 28 degrees C. The delay or inhibition of seed germination at high temperatures may reduce seedling emergence and stand establishment of lettuce in the field, leading to a reduction in economic yield. To identify heat-tolerant lettuce genotypes, lettuce varieties and germplasm accessions were screened for the ability to germinate under high-temperature stress. Twenty-four to 26 genotypes were selected from each lettuce types (crisphead, romaine, butterhead, loose leaf, and wild species) and their seeds were placed in petri dishes to test their ability to germinate at high temperatures (29 and 34 degrees C) as compared with controls at 24 degrees C. Some lettuce genotypes showed thermotolerance to 34 degrees C (less than 20% reduction in germination) such as Elizabeth, PI 342533, PI 358025, Florida Buttercrisp, Kordaat, Corsair, FL 50105, PRO 425, PI 278070, Noemie, Picarde, Gaillarde, L. serriola (PI 491112, UC96US23, PI 491147), L. virosa (PI 274378 D), L. saligna (PI 491159), and primitive (PI 187238 A, PI 289063 C). The germination rates were consistent with the germination percentage at the high temperatures. Seed germination in the field was very low and positively correlated with seed germination at 29 and 34 degrees C. The highest field germination percentages (greater than 40%) were observed in Belluro, Mantilia, Mid Queen, Headmaster, PRO 874, PRO 425, FL 50105, Corsair, Romaine SSC 1148, Romaine Romea, Green Forest, Grenadier, FL 43007, Squadron, Xena, Noemie, Green Wave, Picarde, and Red Giant. The results of this study indicated that lettuce genotypes differ greatly in their ability to germinate at high temperatures as determined by the percentages and the rates of germination. Our research indicates that thermoinsensitive varieties could be used to expand lettuce production seasons in warm and low land cost areas and reduce the need for seed priming, lowering the production costs. The information may also be useful for growers to better choose cultivars for warm environments and for lettuce breeders to improve the crop for adaptation to global warming and climate change. C1 [Lafta, Abbas; Mou, Beiquan] ARS, USDA, Salinas, CA 93905 USA. RP Mou, BQ (reprint author), ARS, USDA, 1636 East Alisal St, Salinas, CA 93905 USA. EM beiquan.mou@ars.usda.gov NR 34 TC 3 Z9 3 U1 2 U2 26 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2013 VL 48 IS 6 BP 708 EP 714 PG 7 WC Horticulture SC Agriculture GA 168BU UT WOS:000320681500006 ER PT J AU Palla, KJ Beasley, RR Pijut, PM AF Palla, Kaitlin J. Beasley, Rochelle R. Pijut, Paula M. TI In Vitro Culture and Rooting of Diospyros virginiana L. SO HORTSCIENCE LA English DT Article DE common persimmon; plant tissue culture; rooting; vitro ID JAPANESE PERSIMMON CULTIVARS; INVITRO; SHOOT; PHLOROGLUCINOL; PRUNUS; AVIUM AB The hard, strong, very close-grained wood of common persimmon (Diospyros virginiana L.; Ebenaceae) is desirable for specialty products such as golf club heads, percussion sticks, billiard cues, and for wood turnery. The edible fruit of cultivated varieties is sold as pulp for use in puddings, cookies, cakes, and custards. Persimmon is usually propagated by grafting. Own-rooted clonal persimmon could offer several advantages to specialty fruit growers such as elimination of grafting, graft incompatibility issues, and improved rootstocks for variety testing. Four mature, grafted (male and female) persimmon genotypes and one hybrid were used for nodal explant culture. Nodal stem explants were cultured on Murashige and Skoog (MS) medium containing 10 mu M zeatin, 3% (w/v) sucrose, and 0.7% (w/v) Bacto agar. Explants were routinely transferred to fresh medium every 3 weeks until shoot cultures were established. All nodal explants excised from grafted greenhouse plants produced at least one viable shoot. For in vitro rooting of microshoots, half-strength MS medium with 0, 5, 10, or 15 mu M indole-3-butyric acid (IBA), 0.1 g.L-1 phloroglucinol, 3% (w/v) sucrose, and 0.7% (w/v) Bacto agar were tested with a 10-day dark culture treatment followed by culture in the light. Best rooting (14% to 87%) was achieved on medium containing 5 mu M IBA for the common persimmon genotypes with means averaging from 0.5 to 3.9 roots per shoot. Ninety-one percent rooting with 5.3 +/- 2.6 roots per shoot was achieved for the hybrid persimmon. Rooted plants were successfully acclimatized to the greenhouse. C1 [Palla, Kaitlin J.; Beasley, Rochelle R.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, Paula M.] USDA Forest Serv, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. RP Pijut, PM (reprint author), USDA Forest Serv, No Res Stn, HTIRC, 715 West State St, W Lafayette, IN 47907 USA. EM ppijut@purdue.edu RI Beasley, Rochelle/M-1396-2015; Pijut, Paula/N-6789-2015 OI Beasley, Rochelle/0000-0001-7325-4085; FU Indiana Nut Growers Association FX We thank Jerome (Jerry) W. Lehman and members of the Indiana Nut Growers Association for partial financial support of this research. We thank Drs. Brian Maynard and Valerie Pence for, their constructive review and suggestions for the improvement of this manuscript. NR 16 TC 0 Z9 0 U1 0 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2013 VL 48 IS 6 BP 747 EP 749 PG 3 WC Horticulture SC Agriculture GA 168BU UT WOS:000320681500013 ER PT J AU Jones, GD Allen, KC AF Jones, G. D. Allen, K. C. TI Pollen analyses of tarnished plant bugs SO PALYNOLOGY LA English DT Article DE pollen; entomopalynology; tarnished plant bugs; USA ID BOLL-WEEVILS COLEOPTERA; HOST PLANTS; FORAGING RESOURCES; LYGUS-LINEOLARIS; CORN-EARWORM; MIRIDAE; LEPIDOPTERA; NOCTUIDAE; HETEROPTERA; MISSISSIPPI AB Wild host plants are important for tarnished plant bug (TPB - Lygus lineolaris) populations, especially when cultivated crops are not flowering. Knowledge of native habitats is important for managing this insect pest. Although pollen has been used to characterize dispersal and food sources of many insects, it has not been used for TPB. The purpose of this study was to determine if pollen analyses could be used as a tool to determine the non-crop plants associated with TPB. Thirty-eight TPB were collected from light traps set up at two sites near the USDA-ARS research farm at Stoneville, Mississippi on 27 June 2011. Overall, 1183 pollen grains from 79 plant taxa were found in the samples. The pollen recovered was identified to 29 families, 33 genera, and 24 species. Many of the taxa of pollen found, such as Saururus cernuus C. Linnaeus (lizard's tail), Sagittaria, Echinodorus and Carya aquatic (water hickory), occur in wet habitats. From the assemblage of identified pollen grains, TBP from both sites utilized plants from disturbed and/or wet habitats. C1 [Jones, G. D.] ARS, USDA, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. [Allen, K. C.] ARS, USDA, So Insect Management Res Unit, Stoneville, MS 38776 USA. RP Jones, GD (reprint author), ARS, USDA, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. EM gretchen.jones@ars.usda.gov FU Cotton Foundation [5001-005-00] FX We thank Ester F. Wilson (retired, USDA-ARS, APMRU) and Chris Parker (USDA-ARS, APMRU) for their dedication and hard work on this project. We thank the Cotton Foundation for funding this research (General Project #5001-005-00). The mention of trade names or commercial products is solely for the purpose of providing specific information, and does not imply recommendation of endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 1 Z9 1 U1 0 U2 9 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0191-6122 J9 PALYNOLOGY JI Palynology PD JUN 1 PY 2013 VL 37 IS 1 BP 170 EP 176 DI 10.1080/01916122.2013.763508 PG 7 WC Plant Sciences; Paleontology SC Plant Sciences; Paleontology GA 161GH UT WOS:000320180000011 ER PT J AU White, SN Knowles, DP AF White, Stephen N. Knowles, Donald P. TI Expanding Possibilities for Intervention against Small Ruminant Lentiviruses through Genetic Marker-Assisted Selective Breeding SO VIRUSES-BASEL LA English DT Review DE small ruminant lentivirus; susceptibility; marker-assisted selection; sheep; goats; TMEM154 ID CAPRINE ARTHRITIS-ENCEPHALITIS; MAEDI-VISNA VIRUS; OVINE PROGRESSIVE PNEUMONIA; LONG TERMINAL REPEAT; PRION PROTEIN GENE; POLYMERASE-CHAIN-REACTION; ENDEMIC LIVESTOCK DISEASES; NATURALLY INFECTED SHEEP; NATURAL SCRAPIE; PRP GENE AB Small ruminant lentiviruses include members that infect sheep (ovine lentivirus [OvLV]; also known as ovine progressive pneumonia virus/maedi-visna virus) and goats (caprine arthritis encephalitis virus [CAEV]). Breed differences in seroprevalence and proviral concentration of OvLV had suggested a strong genetic component in susceptibility to infection by OvLV in sheep. A genetic marker test for susceptibility to OvLV has been developed recently based on the TMEM154 gene with validation data from over 2,800 sheep representing nine cohorts. While no single genotype has been shown to have complete resistance to OvLV, consistent association in thousands of sheep from multiple breeds and management conditions highlight a new strategy for intervention by selective breeding. This genetic marker-assisted selection (MAS) has the potential to be a useful addition to existing viral control measures. Further, the discovery of multiple additional genomic regions associated with susceptibility to or control of OvLV suggests that additional genetic marker tests may be developed to extend the reach of MAS in the future. This review will cover the strengths and limitations of existing data from host genetics as an intervention and outline additional questions for future genetic research in sheep, goats, small ruminant lentiviruses, and their host-pathogen interactions. C1 [White, Stephen N.; Knowles, Donald P.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [White, Stephen N.; Knowles, Donald P.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP White, SN (reprint author), Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. EM Stephen.White@ars.usda.gov; Don.Knowles@ars.usda.gov OI White, Stephen N./0000-0001-9255-6054 NR 224 TC 8 Z9 9 U1 2 U2 25 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4915 J9 VIRUSES-BASEL JI Viruses-Basel PD JUN PY 2013 VL 5 IS 6 BP 1466 EP 1499 DI 10.3390/v5061466 PG 34 WC Virology SC Virology GA 169HE UT WOS:000320769900006 PM 23771240 ER PT J AU Mason, M Cuadra, EJ Elsasser, TH Lopez, J Yoonsung, J AF Mason, M. Cuadra, E. J. Elsasser, T. H. Lopez, J. Yoonsung, J. TI Evaluating the interaction between progesterone, tumor necrosis factor-alpha and cortisol on early loss of transferred embryo in beef cows SO CANADIAN JOURNAL OF ANIMAL SCIENCE LA English DT Article DE PGF(2a); prostaglandin F-2alpha; TNF-alpha; cortisol; progesterone; survival transferred embryos ID PREGNANCY RATE; CORPUS-LUTEUM; DAIRY-COWS; MELENGESTROL ACETATE; IFN-TAU; ESTRUS; CATTLE; INSEMINATION; RECIPIENTS; SURVIVAL AB Fifty-eight non-lactating cows previously synchronized for estrus were assigned to two treatments to assess the effects of progesterone supplementation and its correlation with tumor necrosis factor-alpha (TNF-alpha) and cortisol on the survival of the transferred embryos. On day 7 after exhibiting estrus (day 0), cows in both groups received embryos. In contrast with the control group, animals in the CIDR-group had a controlled internal drug release (CIDR) additionally inserted. Blood samples for progesterone, TNF-alpha and cortisol analysis were taken immediately before insertion and removal of CIDRs and 7 d after insertion. Progesterone did not differ between the control and the CIDR animals at any day of the study; however, it significantly increased at 7 and 14 d after insertion of the embryos in the control animals, compared with the levels observed in that same experimental group at the time of the transfer. Regardless of the treatment, all pregnant cows experienced a significant increase in progesterone from day 0 to day 7. Progesterone on day 0 was correlated to itself (r = 0. 4 6) on day 14 and to TNF-alpha(r = 0.37) on day 0 in pregnant animals; TNF-alpha on day 7 was significantly higher in pregnant cows compared with non-pregnant and correlated between day 0 and day 14. These results suggest that high levels of progesterone during the first 14 d after the transfer are indicative of the survival of transferred embryos. Additionally, these data also indicate that the decrease in TNF-alpha concentration on day 7 after the transfer of embryos may be associated with the low concentrations of progesterone observed in the non-pregnant animals. C1 [Mason, M.; Cuadra, E. J.] Alcorn State Univ, Dept Agr, Lorman, MS 39096 USA. [Elsasser, T. H.] USDA ARS, Bovine Funct Genom Lab, Beltsville Agr Ctr, Beltsville, MD 20705 USA. [Lopez, J.] Univ Nacl Agr, Managua, Nicaragua. [Yoonsung, J.] Prairie View A&M Univ, Cooperat Agr Res Ctr, Prairie View, TX 77446 USA. RP Mason, M (reprint author), Alcorn State Univ, Dept Agr, 1000 ASU Dr 750, Lorman, MS 39096 USA. EM cuadra@alcorn.edu NR 54 TC 0 Z9 0 U1 0 U2 2 PU AGRICULTURAL INST CANADA PI OTTAWA PA 280 ALBERT ST, SUITE 900, OTTAWA, ONTARIO K1P 5G8, CANADA SN 0008-3984 J9 CAN J ANIM SCI JI Can. J. Anim. Sci. PD JUN PY 2013 VL 93 IS 2 BP 217 EP 225 DI 10.4141/CJAS2012-099 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA 168ZE UT WOS:000320745700005 ER PT J AU Bazan, RA Wilcox, BP Munster, C Gary, M AF Bazan, Roberto A. Wilcox, Bradford P. Munster, Clyde Gary, Marcus TI Removing woody vegetation has little effect on conduit flow recharge SO ECOHYDROLOGY LA English DT Article DE recharge; woody plant encroachment; karst; cave hydrology; juniper ID SCALE RAINFALL SIMULATION; RANGELANDS; WATER; STREAMFLOW; EVAPOTRANSPIRATION; CANOPY; RUNOFF; COVER; TEXAS AB In drylands across the globe, grasslands and savannas have succumbed to encroachment by woody plants. There is a concern that, in some cases, these changes may lead to lower groundwater recharge and streamflow. In karst landscapes, the effect of woody plants on recharge is difficult to determine because of the shallow and rocky soils. In our study, we estimated the amount of water entering a shallow cave (3-5m deep) as a surrogate measurement for groundwater recharge, to evaluate whether the removal of Ashe juniper (Juniperus ashei) above the cave would affect recharge. Three sets of large-scale rainfall simulations were conducted in 2005, before removal of the overstory juniper; seven were conducted in 2008, soon after the juniper were removed; and two were conducted in 2009, one year after juniper removal. We found that recharge occurred mainly via conduits or macropores and, as such, was extremely dynamic and responsive to rainfall. The amount of recharge ranged from 3% to 17% of the water applied, the higher percentages being measured when antecedent soil conditions were wet. At least in this case of recharge taking place via conduit flow, removal of the juniper had little if any effect. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Bazan, Roberto A.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Wilcox, Bradford P.; Munster, Clyde] Texas A&M Univ, College Stn, TX 77803 USA. [Gary, Marcus] Edwards Aquifer Author, San Antonio, TX 78215 USA. RP Wilcox, BP (reprint author), Texas A&M Univ, College Stn, TX 77803 USA. EM bwilcox@tamu.edu FU National Science Foundation; Edwards Aquifer Authority; Department of Defense FX The National Science Foundation, the Edwards Aquifer Authority and the Department of Defense provided the funding for this study. In addition, we are grateful to the US Department of Defense for providing access to the site and logistical support. Finally, we are indebted to the following individuals for their contributions to the success of this project: Keith Owens, Bev Shade, Lucas Gregory, Phillip Taucer, Aaron Hoff, Andrew Weichert and George Veni. NR 31 TC 2 Z9 2 U1 1 U2 34 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 J9 ECOHYDROLOGY JI Ecohydrology PD JUN PY 2013 VL 6 IS 3 BP 435 EP 443 DI 10.1002/eco.1277 PG 9 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 165FQ UT WOS:000320469000011 ER PT J AU Tillman, PG AF Tillman, P. G. TI Likelihood of Stink Bugs Colonizing Crops: A Case Study in Southeastern Farmscapes SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE southern green stink bug; brown stink bug; green stink bug ID NEZARA-VIRIDULA HEMIPTERA; HETEROPTERA-PENTATOMIDAE; SEASONAL ABUNDANCE; EUSCHISTUS-SERVUS; IMMATURE STAGES; SOUTH-CAROLINA; COTTON; CORN; ADJACENT; PEANUT AB Stink bugs, including Nezara viridula (L.), Euschistus servus (Say), and Chinavia hilaris (Say), are economic pests across agricultural farmscapes where they can colonize closely associated crops. This 4-yr on-farm study was conducted to examine the likelihood of these three stink bug species colonizing crops in corn-cotton, corn-peanut-cotton, and peanut-cotton farmscapes by using odds ratios. Corn (Zea mays L.), peanut (Arachis hypogaea L.), and cotton (Gossypium hirsutum L.) served as host plants for E. servus and N. viridula. Corn did not serve as a host plant for C. hilaris. Although peanut was a relatively poor host plant, cotton was a relatively good host plant for this stink bug. For N. viridula and E. servus adults, the risk of crop colonization was higher for peanut in peanut-cotton farmscapes with corn than without corn and was highest for cotton in corn-peanut-cotton, followed by peanutDcotton, and lastly corn-cotton farmscapes. The likelihood of oviposition by E. servus, though, was higher in cotton in corn-cotton than peanutDcotton farmscapes. For C. hilaris adults, the risk of crop colonization was highest for cotton in peanutDcotton, followed by corn-peanut-cotton, and lastly corn-cotton farmscapes. Corn was more likely than peanut or cotton to harbor adults and immatures, i.e., egg masses and young nymphs, of N. viridula and E. servus. Adults of all three stink bug species colonized cotton more often than peanut in peanutDcotton farmscapes. However, oviposition by N. viridula and E. servus occurred more often in peanut than in cotton. These assessments of the likelihood of stink bug colonization are essential for modeling predictions of stink bug colonization and designing more comprehensive landscape management approaches for control of stink bugs in these farmscapes. C1 USDA ARS, Crop Protect & Management Lab, Tifton, GA 31793 USA. RP Tillman, PG (reprint author), USDA ARS, Crop Protect & Management Lab, POB 748, Tifton, GA 31793 USA. EM glynn.tillman@ars.usda.gov NR 37 TC 9 Z9 9 U1 3 U2 20 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 438 EP 444 DI 10.1603/EN12269 PG 7 WC Entomology SC Entomology GA 160HL UT WOS:000320109500005 PM 23726052 ER PT J AU Wang, XG Levy, K Nadel, H Johnson, MW Blanchet, A Argov, Y Pickett, CH Daane, KM AF Wang, Xin-Geng Levy, Karmit Nadel, Hannah Johnson, Marshall W. Blanchet, Arnaud Argov, Yael Pickett, Charles H. Daane, Kent M. TI Overwintering Survival of Olive Fruit Fly (Diptera: Tephritidae) and Two Introduced Parasitoids in California SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Bactrocera oleae; biological control; parasitoid; Psyttalia; overwintering ID PSYTTALIA-CONCOLOR HYMENOPTERA; BACTROCERA-OLEAE DIPTERA; BIOLOGICAL-CONTROL AGENT; BRACON-CELER; PERFORMANCE; TEMPERATURES; POPULATIONS; MANAGEMENT; MORTALITY; DIAPAUSE AB The overwintering survival and development of olive fruit fly, Bactrocera oleae (Rossi), and the endoparasitoids, Psyttalia humilis Silvestri and P. lounsburyi (Silvestri), were investigated at sites in California's interior valley and coastal region. In the interior valley, adult flies survived up to 4-6 mo during the winter when food was provided. Adult female flies could oviposit in late fall and early winter on nonharvested fruit and, although egg survival was low (0.23-8.50%), a portion of the overwintered cohort developed into adults the following spring; percentage of survival was negatively correlated to daily minimum temperature. P. humilis and P. lounsburyi successfully oviposited into host larvae in late fall, and their progeny developed into adults the following spring, although with a low percentage (0-11.9%) survivorship. Overwintering survival of puparia of the olive fruit fly and immature larvae of P. humilis and P. lounsburyi (inside host puparia), buried in the soil, were tested at an interior valley and coastal site. Survival of olive fruit fly ranged from 0 to 60% and was affected by the trial date and soil moisture. Overwintering survival of both the fruit fly and tested parasitoids was lower at the colder interior valley than the coastal site; P. humilis immature stages had the highest mortality levels while B. oleae pupae had the lowest mortality levels. The spring emergence pattern of the tested insects was well predicted by a degree-day model. We discuss factors potentially impeding establishment of introduced olive fruit fly parasitoids in California and elsewhere. C1 [Wang, Xin-Geng; Levy, Karmit; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Nadel, Hannah] Agr & Plant Hlth Inspect Serv, USDA, Buzzards Bay, MA 02542 USA. [Johnson, Marshall W.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Blanchet, Arnaud] USDA ARS, European Biol Control Lab, F-34980 Montferrier Sur Lez, France. [Argov, Yael] Israel Cohen Inst Biol Control, Plants Prod & Mkt Board, Citrus Div, IL-50250 Bet Dagan, Israel. [Pickett, Charles H.] Calif Dept Food & Agr, Sacramento, CA 95832 USA. RP Daane, KM (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 137 Mulford Hall, Berkeley, CA 94720 USA. EM kdaane@ucanr.edu FU California Specialty Crop Block Grant; USDA FX We thank Brianne Crabtree, John Hutchins (University of California, Berkeley), and Martha Gerik (University of California, Riverside) for assistance. Funds were provided by the California Specialty Crop Block Grant (administered by the California Department of Food and Agriculture, with funding from USDA), the California Olive Committee, and the USDA CSREES Special Grants Program: Pest Management Alternatives. Voucher specimens are deposited at the UC Berkeley Essig Museum. NR 51 TC 7 Z9 7 U1 0 U2 29 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 467 EP 476 DI 10.1603/EN12299 PG 10 WC Entomology SC Entomology GA 160HL UT WOS:000320109500009 PM 23726056 ER PT J AU Foss, AR Mattson, WJ Trier, TM AF Foss, Anita R. Mattson, William J. Trier, Terry M. TI Effects of Elevated CO2 Leaf Diets on Gypsy Moth (Lepidoptera: Lymantriidae) Respiration Rates SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE gypsy moth; paper birch; trembling aspen; elevated CO2 effects; insect metabolic rate ID ASPEN POPULUS-TREMULOIDES; INSECT HERBIVORE INTERACTIONS; ENRICHED ATMOSPHERIC CO2; HOST-PLANT; NUTRITIONAL INDEXES; FOLIAR QUALITY; FOOD UTILIZATION; DYNAMIC ACTION; PERFORMANCE; O-3 AB Elevated levels of CO2 affect plant growth and leaf chemistry, which in turn can alter host plant suitability for insect herbivores. We examined the suitability of foliage from trees grown from seedlings since 1997 at Aspen FACE as diet for the gypsy moth (Lymantria dispar L.) Lepidoptera: Lymantriidae: paper birch (Betula papyrifera Marshall) in 2004-2005, and trembling aspen (Populus tremuloides Michaux) in 2006-2007, and measured consequent effects on larval respiration. Leaves were collected for diet and leaf chemistry (nutritional and secondary compound proxies) from trees grown under ambient (average 380 ppm) and elevated CO2 (average 560 ppm) conditions. Elevated CO2 did not significantly alter birch or aspen leaf chemistry compared with ambient levels with the exception that birch percent carbon in 2004 and aspen moisture content in 2006 were significantly lowered. Respiration rates were significantly higher (15-59%) for larvae reared on birch grown under elevated CO2 compared with ambient conditions, but were not different on two aspen clones, until larvae reached the fifth instar, when those consuming elevated CO2 leaves on clone 271 had lower (26%) respiration rates, and those consuming elevated CO2 leaves on clone 216 had higher (36%) respiration rates. However, elevated CO2 had no apparent effect on the respiration rates of pupae derived from larvae fed either birch or aspen leaves. Higher respiration rates for larvae fed diets grown under ambient or elevated CO2 demonstrates their lower efficiency of converting chemical energy of digested food stuffs extracted from such leaves into their biosynthetic processes. C1 [Foss, Anita R.; Mattson, William J.] US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI 54501 USA. [Trier, Terry M.] Grand Valley State Univ, Dept Biol, Allendale, MI 49401 USA. RP Foss, AR (reprint author), US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, 5985 Highway K, Rhinelander, WI 54501 USA. EM afoss@fs.fed.us NR 84 TC 3 Z9 3 U1 3 U2 26 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 503 EP 514 DI 10.1603/EN12074 PG 12 WC Entomology SC Entomology GA 160HL UT WOS:000320109500012 PM 23726059 ER PT J AU Lee, DH Wright, SE Boiteau, G Vincent, C Leskey, TC AF Lee, Doo-Hyung Wright, Starker E. Boiteau, Gilles Vincent, Charles Leskey, Tracy C. TI Effectiveness of Glues for Harmonic Radar Tag Attachment on Halyomorpha halys (Hemiptera: Pentatomidae) and Their Impact on Adult Survivorship and Mobility SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE brown marmorated stink bug; dispersal ecology; movement; tracking; invasive species ID FLIGHT PERFORMANCE; INSECT FLIGHT; BENEFICIAL INSECTS; TREE FRUIT; TRACKING; DISPERSAL; PESTS; MILL; COLEOPTERA; MOVEMENTS AB We evaluated the effectiveness of three cyanoacrylate glues (trade names: Krazy [Elmer's Products Inc., Westerville, OH], Loctite [Henkel Corporation, Rocky Hill, CT], and FSA [Barnes Distribution, Cleveland, OH]) to attach harmonic radar tags securely on adult Halyomorpha halys (Stal) (Hemiptera: Pentatomidae) and quantified the effect of the radar tag attachment on insect survivorship and mobility. In the laboratory, the strength of the glue bond between the radar tag and H. halys pronotum was significantly increased when the pronotum was sanded to remove cuticular waxes. The adhesive bond of the radar tag to the sanded pronotum of H. halys had strength of 160-190-g force and there was no significant difference among the three types of glue tested. The three glues had no measurable effect on the survivorship of radar-tagged H. halys over 7 d, compared with untagged insects. Over a 7-d period in the laboratory, horizontal distance traveled, horizontal walking velocity, and vertical climbing distance were all unaffected by the presence of the tags regardless of glue. A field experiment was conducted to compare the free flight behavior of untagged and radar-tagged H. halys. Adults were released on a vertical dowel and their flights were tracked visually up to approximate to 200 m from the release point. There was no significant difference in take-off time or in flight distance, time, or speed between untagged and radar-tagged individuals. In addition, prevailing flight direction was not significantly different between untagged and radar-tagged individuals. The absence of measurable impact of the radar tag attachment on H. halys survivorship or mobility validates the use of harmonic radar tags to study the dispersal ecology of this insect in field conditions. C1 [Lee, Doo-Hyung; Wright, Starker E.; Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Boiteau, Gilles] Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada. [Vincent, Charles] Agr & Agri Food Canada, Hort Res & Dev Ctr, St Jean, PQ J3B 3E6, Canada. RP Lee, DH (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM doohyung.lee@ars.usda.gov FU USDA-NIFA SCRI [2011-51181-30937] FX We thank Cameron Scorza for excellent technical support. This work was supported in part by USDA-NIFA SCRI 2011-51181-30937 award. NR 35 TC 10 Z9 11 U1 2 U2 29 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 515 EP 523 DI 10.1603/EN12320 PG 9 WC Entomology SC Entomology GA 160HL UT WOS:000320109500013 PM 23726060 ER PT J AU Taylor, DB Friesen, K Zhu, JJ AF Taylor, David B. Friesen, Kristina Zhu, Junwei Jerry TI Spatial-Temporal Dynamics of Stable Fly (Diptera: Muscidae) Trap Catches in Eastern Nebraska SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Stomoxys; spatial; sampling; dispersion ID STOMOXYS-CALCITRANS DIPTERA; FLIES DIPTERA; POPULATION-DYNAMICS; SEASONAL ABUNDANCE; FEEDING SITES; HAY; AGGREGATION; SYNCHRONY; DAIRIES; FLORIDA AB Spatial and temporal relationships among catches of adult stable flies, Stomoxys calcitrans (L.), on sticky traps in eastern Nebraska were evaluated. Twenty-five alsynite sticky traps were placed in a 5 by 5 grid with approximate to 1.6-km intervals in a mixed agricultural environment from 2003 to 2011. Denser grids of 45-90 traps were implemented for varying lengths of time during the course of the study. More than two million stable flies were collected over 9 yr. Seasonal abundances based upon total collections from the primary grid of 25 traps were bimodal most years with population peaks in June and September or October. Individual trap catches varied greatly, both spatially and temporally. Trap catches were spatially aggregated with autocorrelation extending to approximate to 2 km. Synchrony among trap catches declined linearly with respect to distance between traps and differences in seasonal distribution increased asymptotically relative to distance between traps. Proximity to confined livestock facilities increased catch and proportion of catch collected later in the season. Fifteen to 20 traps were adequate for estimating stable fly populations with the standard error of the mean equal to 30% of the mean for most of the stable fly season. Early and late in the season, when mean trap catches were low, between 100 and 135 traps would be needed to maintain that level of confidence. Seasonal collection distributions from permutations of subsets of the data with fewer than 24 traps differed significantly from those of the complete grid of 25 traps, indicating that 20 or more traps may be needed to evaluate the seasonal dynamics of a stable fly population. C1 [Taylor, David B.; Friesen, Kristina; Zhu, Junwei Jerry] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Dept Entomol, Lincoln, NE 68583 USA. RP Taylor, DB (reprint author), Univ Nebraska, Rm 304 Entomology Hall,East Campus, Lincoln, NE 68583 USA. EM dave.taylor@ars.usda.gov RI Taylor, David/G-6025-2014; OI Taylor, David/0000-0002-4378-4867; Zhu, Junwei/0000-0001-9846-9656 NR 34 TC 2 Z9 2 U1 1 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 524 EP 531 DI 10.1603/EN12258 PG 8 WC Entomology SC Entomology GA 160HL UT WOS:000320109500014 PM 23726061 ER PT J AU Foelker, CJ Vandenberg, JD Whitmore, M Fierke, MK AF Foelker, C. J. Vandenberg, J. D. Whitmore, M. Fierke, M. K. TI Modeling Agrilus planipennis (Coleoptera: Buprestidae) Within-Tree Colonization Patterns and Development of a Subsampling Technique SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE ash; emerald ash borer; Fraxinus; nonnative invasive ID EMERALD ASH BORER; TWOLINED CHESTNUT BORER; RED OAK BORER; BILINEATUS COLEOPTERA; SAMPLING METHODS; NORTH-AMERICA; CERAMBYEIDAE; POPULATIONS; FAIRMAIRE; WISCONSIN AB Emerald ash borer, Agrilus planipennis Fairmaire, an insect native to central Asia, was first detected in southeast Michigan in 2002, and has since killed millions of ash trees (Fraxinus spp.) in North America. The objectives of this study were to 1) determine an optimal sampling location on girdled ash trap trees for detection of A. planipennis larvae based on measurements of tree characteristics, and 2) develop a whole-tree estimation method for extrapolating larval densities from subsampled heights. We conducted sampling at 1-m increments, recording larval presence, height on tree bole, bolt diameter, and bark roughness for 58 infested ash trees. Analyzing height and diameter separately, generalized linear mixed models indicated the probability of A. planipennis detection was maximized at 17.2 cm for diameter and increased linearly as vertical height increased. There was also a positive relationship between intermediate bark roughness and A. planipennis presence. Stepwise regression indicated the optimal bolts for extrapolating whole tree larval densities were, in order of importance, at heights of 1-2 m, 4-5 m, 7-8 m, and 0-1 m. Subsampling with just one or two bolts explained 70% and 86%, respectively, of the variance in A. planipennis densities. Our results can be used by resource managers to improve efficiency of detection efforts and estimate infestations of A. planipennis. C1 [Foelker, C. J.; Fierke, M. K.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Vandenberg, J. D.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Whitmore, M.] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA. RP Foelker, CJ (reprint author), SUNY Coll Environm Sci & Forestry, 1 Forestry Dr, Syracuse, NY 13210 USA. EM cjfoelke@syr.edu FU U.S. Forest Service; New York Department of Environmental Conservation FX We appreciate contributions by J. Carlson, M. Griggs, K. Walters, and J. Welsh. The New York Department of Environmental Conservation, NY Department of Transportation, and New York State Parks field and supervisory personnel provided important field assistance, especially R. Cole, J. Payette, M. Moskalenko, and T. Money. Funding, transportation, and equipment were provided by the U.S. Forest Service and the New York Department of Environmental Conservation. NR 30 TC 3 Z9 3 U1 2 U2 27 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 532 EP 538 DI 10.1603/EN12276 PG 7 WC Entomology SC Entomology GA 160HL UT WOS:000320109500015 PM 23726062 ER PT J AU Ranger, CM Tobin, PC Reding, ME Bray, AM Oliver, JB Schultz, PB Frank, SD Persad, AB AF Ranger, Christopher M. Tobin, Patrick C. Reding, Michael E. Bray, Alicia M. Oliver, Jason B. Schultz, Peter B. Frank, Steven D. Persad, Anand B. TI Interruption of the Semiochemical-Based Attraction of Ambrosia Beetles to Ethanol-Baited Traps and Ethanol-Injected Trap Trees by Verbenone SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE verbenone; ambrosia beetle; Scolytinae; Xylosandrus germanus; Xylosandrus ID MOUNTAIN PINE-BEETLE; DENDROCTONUS-PONDEROSAE COLEOPTERA; LODGEPOLE PINE; CURCULIONIDAE SCOLYTINAE; XYLOSANDRUS-GERMANUS; BARK BEETLE; VOLATILES; ATTACKS; PROTECTION; MANAGEMENT AB We examined the extent to which verbenone, a bark beetle antiaggregation pheromone, interrupted the semiochemical-based attraction of ambrosia beetles. Field trapping studies conducted in Ohio showed that a verbenone dispenser with a release rate of 50 mg/d at 25 degrees C reduced the attraction of Anisandrus sayi Hopkins, Euwallacea validus (Eichhoff), Hypothenemus dissimilis (Zimmermann), Xylosandrus germanus (Blandford), and Xyleborinus saxesenii (Ratzeburg) to ethanol-baited traps. A verbenone dispenser attached to ethanol-injected Magnolia virginiana L. trap trees deployed in Ohio also reduced ambrosia beetle attacks compared to trap trees without a verbenone dispenser. Subsequent field trials demonstrated a direct relationship between distance from a verbenone dispenser and ambrosia beetle attacks on trap trees in Ohio in 2011 and 2012 and Tennessee in 2012, but not in Tennessee and Virginia in 2011. Assessment of the inauence of verbenone on the probability of attacks above a density threshold found that although attacks occurred on trap trees regardless of their proximity to a verbenone dispenser, the higher density of attacks per tree occurred on trap trees farthest away from the verbenone source in Ohio and Tennessee. Verbenone alone could be somewhat useful for discouraging ambrosia beetle attacks on individual trees or on a small spatial scale, but deployment of verbenone might be most effective when integrated as part of a "push-pull" strategy. C1 [Ranger, Christopher M.; Reding, Michael E.] USDA ARS, Applicat Technol Res Unit, Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Tobin, Patrick C.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. [Bray, Alicia M.; Oliver, Jason B.] Tennessee State Univ, Coll Agr Human Nat Sci, Otis L Floyd Nursery Res Ctr, McMinnville, TN 37110 USA. [Schultz, Peter B.] Virginia Tech, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA 23455 USA. [Frank, Steven D.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Persad, Anand B.] Davey Tree Inst, Kent, OH 44240 USA. RP Ranger, CM (reprint author), USDA ARS, Applicat Technol Res Unit, Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU USDA Floriculture and Nursery Research Initiative; ARS Research Project (National Program 304-Crop Protection and Quarantine) [3607-22000-012-00D]; Horticultural Research Institute; North Carolina Nursery and Landscape Association FX This research was supported in part by funding from the USDA Floriculture and Nursery Research Initiative and base funds associated with ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine), along with the Horticultural Research Institute, and the North Carolina Nursery and Landscape Association. We thank James Moyseenko, Jennifer Barnett, and Betsy Anderson (USDA-ARS) for technical assistance in Ohio, and Nadeer Youssef, Joshua Basham, Debbie Eskandarnia, and Joseph Lampley (Tennessee State University College of Agriculture, Human, and Natural Sciences) for technical assistance in Tennessee. We thank Jan Meneley (AgBio Inc.) for providing the ethanol and verbenone dispensers, and Adcock's Nursery (Fuquay-Varina, NC) for allowing S. D. F. to conduct a trapping study at their nursery. We also thank anonymous reviewers for useful comments. Mention of proprietary products or companies does not imply any endorsement or preferential treatment by the USDA-Agricultural Research Service, USDA-Forest Service, The Ohio State University, North Carolina State University, Tennessee State University, VA Tech, or the Davey Tree Institute. NR 43 TC 7 Z9 8 U1 1 U2 35 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 539 EP 547 DI 10.1603/EN13016 PG 9 WC Entomology SC Entomology GA 160HL UT WOS:000320109500016 PM 23726063 ER PT J AU Jordan, TA Zhang, A Pfeiffer, DG AF Jordan, T. A. Zhang, A. Pfeiffer, D. G. TI Blend Chemistry and Field Attraction of Commercial Sex Pheromone Lures to Grape Berry Moth (Lepidoptera: Tortricidae), and a Nontarget Tortricid in Vineyards SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE pest management; nontarget species; sampling; logistic regression ID VITEANA CLEMENS LEPIDOPTERA; MATING DISRUPTION; PARALOBESIA-VITEANA; NEW-YORK; PHENOLOGY; HABITATS; TRAPS AB Anecdotal reports by scientists and growers suggested commercial sex pheromone lures were ineffective with monitoring field populations of grape berry moth, Paralobesia viteana (Clemens), in vineyards. This study addressed the need to evaluate commercial sex pheromone lures for chemical purity and efficacy of attracting grape berry moth and a nontarget tortricid, the sumac moth, Episumus argutanus (Clemens). The percentage of chemical components from a set of eight lures from each manufacturer was found using gas chromatography-mass spectrometry and confirmed by chemical standards. No lures adhered to the 9:1 blend of (Z)-9-dodecenyl acetate (Z9-12:Ac) to (Z)-11-tetradecenyl acetate (Z11-14:Ac), though Suterra (9.1:1), ISCA (5.7:1), and Trece (5.4:1) lures were closest. The Trece lures contained approximate to 98 mu g Z9-12:Ac, which is 3-51 times more than the other lures. The Suterra and ISCA lures were loaded with approximate to 29 and 33 mu g Z9-12:Ac, and the Alpha Scents lures only contained approximate to 2 mu g Z9-12:Ac. Anantagonistic impurity, (E)-9-tetradecenyl acetate (E9-12:Ac), was found in all manufacturer lures at concentrations from 3.2 to 4.8%. Field attraction studies were done in summer 2010, and again in 2011, to evaluate commercial lures for their potential to attract P. viteana and E. argutanus in the presence of lures from other manufacturers. Separate experiments were established in two vineyards in Augusta County, VA, one with open and the other with wooded surroundings. In field experiments, Suterra lures detected P. viteana most often, Trece lures detected more E. argutanus, and ISCA lures detected P. viteana in the open vineyard the least, while Alpha Scents lures were least attractive to E. argutanus in both environments. Fewer P. viteana were captured in the wooded versus open vineyard, which may limit the potential for sex pheromone monitoring of P. viteana in wooded vineyards. C1 [Jordan, T. A.; Pfeiffer, D. G.] Virginia Tech, Dept Entomol, Blacksburg, VA 24061 USA. [Zhang, A.] USDA ARS PSI, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Jordan, TA (reprint author), Virginia Tech, Dept Entomol, 216A Price Hall, Blacksburg, VA 24061 USA. EM tajordan@vt.edu FU Virginia Wine Board FX We would like to acknowledge David Wenger of Wenger Grape Farms from Waynesboro, VA, for use of their vineyards during this study. In addition, we acknowledge the guidance of Wandi Huang from the Laboratory for Interdisciplinary Statistical Analysis at Virginia Tech. Lastly, we acknowledge our funding source, the Virginia Wine Board, for support of this research. NR 24 TC 0 Z9 1 U1 2 U2 20 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 558 EP 563 DI 10.1603/EN13033 PG 6 WC Entomology SC Entomology GA 160HL UT WOS:000320109500018 PM 23726065 ER PT J AU Yee, WL AF Yee, Wee L. TI Influence of Media Type and Moisture on Adult Development and Pupal Mortality in Rhagoletis indifferens (Diptera: Tephritidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE western cherry fruit fly; teneral adults; pupae; loam; sand ID FRUIT-FLY DIPTERA; HOST-PLANT USE; SOIL-MOISTURE; APPLE MAGGOT; WASHINGTON-STATE; TEMPERATURE; DESICCATION; SURVIVAL; LARVAE AB The influence of media type and moisture on adult development and pupal mortality in western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), was assessed using the pupal-adult and the larval-pupal stage. Inside containers, a higher percent of flies that emerged from dry loam was deformed (44.2%, 1-cm-depth loam; 84.4%, 5-cm-depth loam) than flies from 16% moist loam and dry and 16% moist lab soil (peat mossDsand mix) (0-14.9%). Percent of flies deformed from dry sand (22.1%, 1-cm depth; 49.5%, 5-cm depth) was greater than from 16% moist sand and dry and 16% moist peat moss (0-10.5%). Percents of aies deformed from 8% moist loam, lab soil, sand, and peat moss (0-5.8%) did not differ. Pupae suffered higher mortality at 7 and 14 d after larvae were dropped onto dry loam and dry sand (68.2-94.0%) than dry lab soil and dry peat moss (3.0-53.0%); respective mortalities at 21 and 28 d were similar (81.3-96.0 versus 64.7-97.9%). Pupal mortality in moist media was lower (0.5-40.3%) than in dry media. In outdoor tests, pupal mortality was also higher in dry loam than other dry media. In nature, 60.9% of pupae in dry sandy loams in late summer were dead. Results suggest R. indifferens has not yet evolved to fully cope with dry soils and that pupation in media with traits similar to those of peat moss or a peat moss-sand mix could reduce negative effects of dry environments on fly survival. C1 USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Yee, WL (reprint author), USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov NR 28 TC 0 Z9 0 U1 3 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2013 VL 42 IS 3 BP 595 EP 604 DI 10.1603/EN12346 PG 10 WC Entomology SC Entomology GA 160HL UT WOS:000320109500023 PM 23726070 ER PT J AU Syme, RA Hane, JK Friesen, TL Oliver, RP AF Syme, Robert Andrew Hane, James K. Friesen, Timothy L. Oliver, Richard P. TI Resequencing and Comparative Genomics of Stagonospora nodorum: Sectional Gene Absence and Effector Discovery SO G3-GENES GENOMES GENETICS LA English DT Article DE Fungal comparative genomics; Fungal effectors; Phytopathogen ID PYRENOPHORA-TRITICI-REPENTIS; HOST-SELECTIVE TOXINS; POINT MUTATION RIP; TRIGGERED SUSCEPTIBILITY; NECROTROPHIC EFFECTORS; WHEAT; DISEASE; ALIGNMENT; PATHOGENICITY; RESISTANCE AB Stagonospora nodorum is an important wheat (Triticum aestivum) pathogen in many parts of the world, causing major yield losses. It was the first species in the large fungal Dothideomycete class to be genome sequenced. The reference genome sequence (SN15) has been instrumental in the discovery of genes encoding necrotrophic effectors that induce disease symptoms in specific host genotypes. Here we present the genome sequence of two further S. nodorum strains (Sn4 and Sn79) that differ in their effector repertoire from the reference. Sn79 is avirulent on wheat and produces no apparent effectors when infiltrated onto many cultivars and mapping population parents. Sn4 is pathogenic on wheat and has virulences not found in SN15. The new strains, sequenced with short-read Illumina chemistry, are compared with SN15 by a combination of mapping and de novo assembly approaches. Each of the genomes contains a large number of strain-specific genes, many of which have no meaningful similarity to any known gene. Large contiguous sections of the reference genome are absent in the two newly sequenced strains. We refer to these differences as "sectional gene absences." The presence of genes in pathogenic strains and absence in Sn79 is added to computationally predicted properties of known proteins to produce a list of likely effector candidates. Transposon insertion was observed in the mitochondrial genomes of virulent strains where the avirulent strain retained the likely ancestral sequence. The study suggests that short-read enabled comparative genomics is an effective way to both identify new S. nodorum effector candidates and to illuminate evolutionary processes in this species. C1 [Syme, Robert Andrew; Oliver, Richard P.] Curtin Univ, Dept Environm & Agr, Australian Ctr Necrotroph Fungal Pathogens, Bentley, WA 6845, Australia. [Hane, James K.] CSIRO, Ctr Environm & Life Sci, Mol Pathol & Plant Pathol Lab, Floreat, WA 6014, Australia. [Friesen, Timothy L.] ARS, USDA, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Oliver, RP (reprint author), Curtin Univ, Dept Environm & Agr, Room 304-109, Perth, WA 6845, Australia. EM richard.oliver@curtin.edu.au RI Hane, James/A-7062-2011 OI Hane, James/0000-0002-7651-0977 FU Australian Grains Research and Development Corporation; iVEC; Australian National University FX We thank Bruce McDonald, Eva Stukenbrock, Viola Manning, and Lynda Ciuffetti for many insightful discussions. This research was supported by the Australian Grains Research and Development Corporation. The work was supported by iVEC through the use of advanced computing resources located at iVEC@Murdoch and by an award under the Merit Allocation Scheme on the NCI National Facility at the Australian National University. NR 70 TC 14 Z9 15 U1 1 U2 39 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2013 VL 3 IS 6 BP 959 EP 969 DI 10.1534/g3.112.004994 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 169GT UT WOS:000320768700005 PM 23589517 ER PT J AU Hirsch, CN Hirsch, CD Felcher, K Coombs, J Zarka, D Van Deynze, A De Jong, W Veilleux, RE Jansky, S Bethke, P Douches, DS Buell, CR AF Hirsch, Candice N. Hirsch, Cory D. Felcher, Kimberly Coombs, Joseph Zarka, Dan Van Deynze, Allen De Jong, Walter Veilleux, Richard E. Jansky, Shelley Bethke, Paul Douches, David S. Buell, C. Robin TI Retrospective View of North American Potato (Solanum tuberosum L.) Breeding in the 20th and 21st Centuries SO G3-GENES GENOMES GENETICS LA English DT Article DE Solanum tuberosum; phenotypic diversity; genotypic diversity; genomics ID GERMPLASM; ACCUMULATION; VARIETY; QUALITY; POLYMORPHISM; CAROTENOIDS; CYCLASE; HYBRIDS; STORAGE; SOLIDS AB Cultivated potato (Solanum tuberosum L.), a vegetatively propagated autotetraploid, has been bred for distinct market classes, including fresh market, pigmented, and processing varieties. Breeding efforts have relied on phenotypic selection of populations developed from intra-and intermarket class crosses and introgressions of wild and cultivated Solanum relatives. To retrospectively explore the effects of potato breeding at the genome level, we used 8303 single-nucleotide polymorphism markers to genotype a 250-line diversity panel composed of wild species, genetic stocks, and cultivated potato lines with release dates ranging from 1857 to 2011. Population structure analysis revealed four subpopulations within the panel, with cultivated potato lines grouping together and separate from wild species and genetic stocks. With pairwise kinship estimates clear separation between potato market classes was observed. Modern breeding efforts have scarcely changed the percentage of heterozygous loci or the frequency of homozygous, single-dose, and duplex loci on a genome level, despite concerted efforts by breeders. In contrast, clear selection in less than 50 years of breeding was observed for alleles in biosynthetic pathways important for market class-specific traits such as pigmentation and carbohydrate composition. Although improvement and diversification for distinct market classes was observed through whole-genome analysis of historic and current potato lines, an increased rate of gain from selection will be required to meet growing global food demands and challenges due to climate change. Understanding the genetic basis of diversification and trait improvement will allow for more rapid genome-guided improvement of potato in future breeding efforts. C1 [Hirsch, Candice N.; Hirsch, Cory D.; Buell, C. Robin] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Felcher, Kimberly; Coombs, Joseph; Zarka, Dan; Douches, David S.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Van Deynze, Allen] Univ Calif Davis, Seed Biotechnol Ctr, Davis, CA 95616 USA. [De Jong, Walter] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Veilleux, Richard E.] Virginia Polytech Inst & State Univ, Dept Hort, Blacksburg, VA 24061 USA. [Jansky, Shelley; Bethke, Paul] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Jansky, Shelley; Bethke, Paul] ARS, USDA, Vegetable Crops Res Unit, Madison, WI USA. RP Buell, CR (reprint author), Michigan State Univ, Dept Plant Biol, 612 Wilson Rd, E Lansing, MI 48824 USA. EM buell@msu.edu OI Hirsch, Cory/0000-0002-3409-758X FU U.S. Department of Agriculture; National Institute of Food and Agriculture; Food Research Initiative Plant Breeding, Genetics, and Genome [2009-85606-05673]; National Science Foundation National Plant Genome Initiative [1202724] FX We thank David Spooner for the suggestion of wild species to include in the panel and Michael Hardigan for growing the wild species. We also thank Greg Porter, Craig Yencho, Kathleen Haynes, Jiwan Palta, John Bamberg, Dave Holm, Creighton Miller, Benoit Bizimungu, Christian Thill, Asunta Thompson, Rich Novy, Charles Brown, and Liping Jin for contributing lines to the panel. We thank David Francis for help in experimental design and conception and Kevin Stoffel for help in genotyping. This work was supported by funds from the U.S. Department of Agriculture, National Institute of Food and Agriculture, Agriculture and Food Research Initiative Plant Breeding, Genetics, and Genome grant 2009-85606-05673 to D.S.D. CDH was supported by a National Science Foundation National Plant Genome Initiative Postdoctoral Fellowship [grant number 1202724]. NR 42 TC 39 Z9 39 U1 0 U2 32 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2013 VL 3 IS 6 BP 1003 EP 1013 DI 10.1534/g3.113.005595 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 169GT UT WOS:000320768700009 PM 23589519 ER PT J AU Bearson, SMD Allen, HK Bearson, BL Looft, T Brunelle, BW Kich, JD Tuggle, CK Bayles, DO Alt, D Levine, UY Stanton, TB AF Bearson, Shawn M. D. Allen, Heather K. Bearson, Bradley L. Looft, Torey Brunelle, Brian W. Kich, Jalusa D. Tuggle, Christopher K. Bayles, Darrell O. Alt, David Levine, Uri Y. Stanton, Thaddeus B. TI Profiling the gastrointestinal microbiota in response to Salmonella: Low versus high Salmonella shedding in the natural porcine host SO INFECTION GENETICS AND EVOLUTION LA English DT Article DE Salmonella; Intestinal microbiota; Swine ID ENTERICA SEROVAR TYPHIMURIUM; SP NOV.; INTESTINAL MICROBIOTA; BACTERIAL DIVERSITY; GUT MICROBIOTA; HUMAN FECES; SWINE; PIGS; COLONIZATION; INFLAMMATION AB Controlling Salmonella in the food chain is complicated by the ability of Salmonella to colonize livestock without causing clinical symptoms/disease. Salmonella-carrier animals are a significant reservoir for contamination of naive animals, the environment, and our food supply. Salmonella carriage and shedding in pigs varies greatly both experimentally and on-farm. To investigate the dynamics between the porcine intestinal microbiota and Salmonella shedding, we temporally profiled the microbiota of pigs retrospectively classified as low and high Salmonella-shedders. Fifty-four piglets were collectively housed, fed and challenged with 10(9) Salmonella enterica serovar Typhimurium. Bacterial quantitation of Salmonella in swine feces was determined, and total fecal DNA was isolated for 16S rRNA gene sequencing from groups of high-shedder, low-shedder, and non-inoculated pigs (n = 5/group; 15 pigs total). Analyses of bacterial community structures revealed significant differences between the microbiota of high-shedder and low-shedder pigs before inoculation and at 2 and 7 days post-inoculation (d.p.i.); microbiota differences were not detected between low-shedder and non-inoculated pigs. Because the microbiota composition prior to Salmonella challenge may influence future shedding status, the "will-be" high and low shedder phylo-types were compared, revealing higher abundance of the Ruminococcaceae family in the "will-be" low shedders. At 2 d.p.i., a significant difference in evenness for the high shedder microbiota compared to the other two groups was driven by decreases in Prevotella abundance and increases in various genera (e.g. Catenibacterium, Xylanibacter). By 21 d.p.i., the microbial communities of high-shedder and low-shedder pigs were no longer significantly different from one another, but were both significantly different from non-inoculated pigs, suggesting a similar Salmonella-induced alteration in maturation of the swine intestinal microbiota regardless of shedding status. Our results correlate microbial shifts with Salmonella shedding status in pigs, further defining the complex interactions among the host, pathogen, and microbiota of this important public health issue and food safety concern. Published by Elsevier B.V. C1 [Bearson, Shawn M. D.; Allen, Heather K.; Looft, Torey; Brunelle, Brian W.; Bayles, Darrell O.; Alt, David; Levine, Uri Y.; Stanton, Thaddeus B.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Bearson, Bradley L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50010 USA. [Kich, Jalusa D.] Embrapa Swine & Poultry, Concordia, SC, Brazil. [Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. RP Bearson, SMD (reprint author), USDA ARS, Natl Anim Dis Ctr, 1920 Dayton Ave,Room 1403, Ames, IA 50010 USA. EM shawn.bearson@ars.usda.gov; heather.allen@ars.usda.gov; brad.bearson@ars.usda.gov; torey.looft@ars.usda.gov; brian.brunelle@ars.usda.gov; jalusa.kich@ars.usda.gov; cktuggle@iastate.edu; darrell.bayles@ars.usda.gov; david.alt@ars.usda.gov; uri.levine@ars.usda.gov; thaddeus.stanton@ars.usda.gov FU USDA, ARS CRIS funds; National Research Initiative competitive grant from the USDA National Institute of Food and Agriculture Animal Genome Program [2009-35205-05192] FX We are grateful for the superb technical assistance of Jennifer Jones, Stephanie Jones and Lea Ann Hobbs and for the critical evaluation of the manuscript by Thomas Casey. This project was supported by USDA, ARS CRIS funds and by National Research Initiative competitive grant no.2009-35205-05192 from the USDA National Institute of Food and Agriculture Animal Genome Program. NR 69 TC 17 Z9 17 U1 6 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD JUN PY 2013 VL 16 BP 330 EP 340 DI 10.1016/j.meegid.2013.03.022 PG 11 WC Infectious Diseases SC Infectious Diseases GA 166PM UT WOS:000320569500044 PM 23535116 ER PT J AU Petzold-Maxwell, JL Jaronski, ST Clifton, EH Dunbar, MW Jackson, MA Gassmann, AJ AF Petzold-Maxwell, Jennifer L. Jaronski, Stefan T. Clifton, Eric H. Dunbar, Mike W. Jackson, Mark A. Gassmann, Aaron J. TI Interactions Among Bt Maize, Entomopathogens, and Rootworm Species (Coleoptera: Chrysomelidae) in the Field: Effects on Survival, Yield, and Root Injury SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Correction DE Bacillus thuringiensis; receptor binding; ELISA; immunohistochemistry; Aedes albopictus AB Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 puried from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoesO brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little difference was found in the test of immunohistochemistry. Cyt1Aa6 was detected in the midguts of both mosquitoes with histopathological changes. It would of great importance to the knowledge of the novel toxin against to Cx. quinquefasciatus and Ae. albopictus. C1 [Petzold-Maxwell, Jennifer L.] Iowa State Univ, Dept Entomol, Ames, IA 50126 USA. [Jaronski, Stefan T.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Jackson, Mark A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Petzold-Maxwell, JL (reprint author), Iowa State Univ, Dept Entomol, 13 Insectary, Ames, IA 50126 USA. EM jen.l.petzold@gmail.com NR 1 TC 0 Z9 0 U1 0 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP V EP V DI 10.1603/EC12308 PG 1 WC Entomology SC Entomology GA 160IA UT WOS:000320111300001 ER PT J AU Rinehart, JP Yocum, GD Kemp, WP Greenlee, KJ AF Rinehart, Joseph P. Yocum, George D. Kemp, William P. Greenlee, Kendra J. TI A Fluctuating Thermal Regime Improves Long-Term Survival of Quiescent Prepupal Megachile rotundata (Hymenoptera: Megachilidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE alfalfa leafcutting bee; Megachile rotundata; insect cold storage; fluctuating thermal regime ID ALFALFA LEAFCUTTING BEE; COLORADO POTATO BEETLE; LEPTINOTARSA-DECEMLINEATA; HIGH-TEMPERATURE; GENE-EXPRESSION; SOLITARY BEE; DIAPAUSE DEVELOPMENT; MAINTENANCE PHASES; COLD INJURY; EMERGENCE AB The alfalfa leafcutting bee Megachile rotundata (F.) is the primary pollinator for alfalfa seed production. Under standard management conditions, the alfalfa leafcutting bee develops to the diapausing prepupal stage undereld conditions, after which they are cold-stored at a static temperature until the following spring, when temperatures are raised and development resumes. Wehave assessed the effects of a auctuating thermal regime (FTR) during overwintering cold storage, where bees were exposed to a daily 1 h pulse of 20 degrees C, and compared viability and insect quality to bees stored under a static thermal regime. Our results demonstrate that implementing an FTR protocol dramatically increases the survival of cold-stored alfalfa leafcutting bees, effectively extending their shelf-life into the subsequent growing season. These ndings could substantially ameliorate signicant obstacles that restrict the more widespread use of this important pollinator, such as the biological constraints that restrict its use in early blooming crops, and yearly auctuations in bee prices that add signicant nancial uncertainty to end users. This study also strengthens a growing body of evidence that indicates FTR protocols are superior to static thermal regime protocols for insect cold storage. C1 [Rinehart, Joseph P.; Yocum, George D.; Kemp, William P.] USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58102 USA. [Greenlee, Kendra J.] N Dakota State Univ, Dept Biol Sci, Fargo, ND 58108 USA. RP Rinehart, JP (reprint author), USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM joseph.rinehart@ars.usda.gov NR 42 TC 8 Z9 8 U1 2 U2 23 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1081 EP 1088 DI 10.1603/EC12486 PG 8 WC Entomology SC Entomology GA 160IA UT WOS:000320111300003 PM 23865170 ER PT J AU Bennett, MM Petersen, K Yocum, G Rinehart, J Kemp, W Greenlee, KJ AF Bennett, Meghan M. Petersen, Kelsey Yocum, George Rinehart, Joseph Kemp, William Greenlee, Kendra J. TI Effects of Extended Prepupal Storage Duration on Adult Flight Physiology of the Alfalfa Leafcutting Bee (Hymenoptera: Megachilidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Megachile rotundata; diapause; thermal stress; respiration; metabolic rate ID METABOLIC-RATE; SCHISTOCERCA-AMERICANA; APIS-MELLIFERA; MANDUCA-SEXTA; BODY-SIZE; ROTUNDATA; LOCUST; MASS; PERFORMANCE; ENERGETICS AB The alfalfa leafcutting bee, Megachile rotundata (F.), is a solitary, cavity-nesting bee and is the primary pollinator for alfalfa seed production. Bee management practices include cold storage during the prepupal stage. Fluctuating thermal regimes during cold storage increases survival of cold storage and allows a doubling of the cold storage period with no decrease in survival. However, survival, characterized as successful adult emergence, is not qualitative. In this study, we determined whether extended storage affects adult bee respiration or aight physiology. Weoverwintered bees for a single winter (current management protocol) or for 12 mo longer (extended storage). We used resting and tethered aight metabolic rates and resting critical PO2 (the oxygen partial pressure below which metabolism can no longer be sustained) as indices of adult bee quality. We found no significant differences in body mass, resting or aight metabolic rates, or critical PO2 between the two groups. Together these data indicate that extended storage of M. rotundata produces bees of similar respiratory capacity and aight ability. These ndings could increase the use of M. rotundata as an alternative pollinator, allowing for extended storage to time adult emergence with early blooming crops. C1 [Bennett, Meghan M.; Petersen, Kelsey; Greenlee, Kendra J.] N Dakota State Univ, Dept Biol Sci, Dept 2715, Fargo, ND 58108 USA. [Yocum, George; Rinehart, Joseph; Kemp, William] USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58102 USA. RP Greenlee, KJ (reprint author), N Dakota State Univ, Dept Biol Sci, Dept 2715, POB 6050, Fargo, ND 58108 USA. EM kendra.greenlee@ndsu.edu FU National Science Foundation [EPS-0447679, IOS-0953297]; National Institutes of Health National Center for Research Resources [2P20RR0l5566] FX The authors thank Carlee O'Dell, Marnie Larson, Lindsey Fransen, Karen Dickey, and Austin Owings for their technical assistance and two anonymous reviewers for their comments on the manuscript. This work was supported by the National Science Foundation (EPS-0447679 and IOS-0953297 to K.J.G.) and the National Institutes of Health National Center for Research Resources (2P20RR0l5566 to K.J.G). The contents of this study are solely the responsibility of the authors and do not necessarily reflect the views of the National Institutes of Health. NR 30 TC 5 Z9 6 U1 1 U2 23 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1089 EP 1097 DI 10.1603/EC12487 PG 9 WC Entomology SC Entomology GA 160IA UT WOS:000320111300004 PM 23865171 ER PT J AU Duan, JJ Bauer, LS Abell, KJ Lelito, JP Van Driesche, R AF Duan, Jian J. Bauer, Leah S. Abell, Kristopher J. Lelito, Jonathan P. Van Driesche, Roy TI Establishment and Abundance of Tetrastichus planipennisi (Hymenoptera: Eulophidae) in Michigan: Potential for Success in Classical Biocontrol of the Invasive Emerald Ash Borer (Coleoptera: Buprestidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE natural enemy introduction; invasive; wood borers; parasitoid release and establishment ID RUSSIAN FAR-EAST; AGRILUS-PLANIPENNIS; NATURAL ENEMIES; BIOTIC FACTORS; CHINA; BRACONIDAE; POPULATIONS; PARASITOIDS; ENCYRTIDAE; DORYCTINAE AB Tetrastichus planipennisi Yang is a gregarious larval endoparasitoid native to China and has been introduced to the United States since 2007 for classical biological control of the invasive emerald ash borer, Agrilus planipennis Fairmaire, an exotic beetle responsible for widespread ash mortality. Between 2007-2010, T. planipennisi adults (3,311-4,597 females and approximate to 1,500 males per site) were released into each of six forest sites in three counties (Ingham, Gratiot, and Shiawassee) of southern Michigan. By the fall of 2012, the proportion of sampled trees with one or more broods of T. planipennisi increased to 92 and 83% in the parasitoid-release and control plots, respectively, from 33 and 4% in the first year after parasitoid releases (2009 fall for Ingham county sites and 2010 for other sites). Similarly, the mean number of T. planipennisi broods observed from sampled trees increased from less than one brood per tree in the first year after parasitoid releases to 2.46 (at control plots) to 3.08 (at release plots) broods by the fall of 2012. The rates of emerald ash borer larval parasitism by T. planipennisi also increased from 1.2% in the first year after parasitoid releases to 21.2% in the parasitoid-release plots, and from 0.2 to 12.8% for the control plots by the fall of 2012. These results demonstrate that T. planipennisi is established in southern Michigan and that its populations are increasing and expanding. This suggests that T. planipennisi will likely play a critical role in suppressing emerald ash borer populations in Michigan. C1 [Duan, Jian J.] USDADARS, Beneficial Insects Introduct Res Unit, Newark, DE 19713 USA. [Bauer, Leah S.] USDA Forest Serv, No Res Stn, E Lansing, MI 48823 USA. [Bauer, Leah S.; Abell, Kristopher J.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Abell, Kristopher J.; Van Driesche, Roy] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Lelito, Jonathan P.] USDA APHIS PPQ Emerald Ash Borer Program, Brighton, MI 48116 USA. RP Duan, JJ (reprint author), USDADARS, Beneficial Insects Introduct Res Unit, 501 South Chapel St, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov FU USDA Forest Service, Forest Health Protection FX We thank Deborah Miller (USDAD-Forest Service), Michael Ulyshen, Jason Hansen, Tim Watt, and Ian Lane (Michigan State University, Department. Entomology), Tony Capizzo and Jane Slater (USDA-ARS) for assistance in laboratory work and field surveys of emerald ash borer larvae and their parasitoids. We are also grateful to Amos Ziegler and Travis Perkins (Michigan State University, Department. Entomology, Applied Spatial Ecology and Technical Services) for assisting with map preparation; Doug Luster and Roger Fuester (USDA-ARS) for helpful comments on an early version of this manuscript; and Richard Reardon and Steven Katovich (USDA Forest Service, Forest Health Protection) for providing some financial support for this project. NR 33 TC 32 Z9 34 U1 3 U2 59 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1145 EP 1154 DI 10.1603/EC13047 PG 10 WC Entomology SC Entomology GA 160IA UT WOS:000320111300011 PM 23865178 ER PT J AU Walse, SS Myers, SW Liu, YB Bellamy, DE Obenland, D Simmons, GS Tebbets, S AF Walse, Spencer S. Myers, Scott W. Liu, Yong-Biao Bellamy, David E. Obenland, David Simmons, Greg S. Tebbets, Steve TI Postharvest Treatment of Fresh Fruit From California With Methyl Bromide for Control of Light Brown Apple Moth (Lepidoptera: Tortricidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Epiphyas postvittana; postharvest fumigation; sorption kinetics; methyl bromide ID EPIPHYAS-POSTVITTANA; NEW-ZEALAND; GAS-DIFFUSION; MODEL; PREFERENCES; SORPTION AB Methyl bromide (MB) chamber fumigations were evaluated for postharvest control of light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), in fresh fruit destined for export from California. To simulate external feeding, larvae were contained in gas-permeable cages and distributed throughout loads of peaches, plums, nectarines (all Prunus spp.), apples (Malus spp.), raspberries (Rubus spp.), or grapes (Vitis spp.). Varying the applied MB dose and the differential sorption of MB by the loads resulted in a range of exposures, expressed as concentration x time cross products (CTs) that were verified by gas-chromatographic quantification of MB in chamber headspace over the course of each fumigation. CTs >= 60 and >= 72 mg liter(-1) h at 10.0 +/- 0.5 and 15.6 +/- 0.5 degrees C ((x) over bar +/- s, average +/- SD), respectively, yielded complete mortality of approximate to 6,200 larvae at each temperature. These confirmatory fumigations corroborate E. postvittana mortality data for the first time in relation to measured MB exposures and collectively comprise the largest numberof larval specimens tested to date. In addition, a kinetic model of MB sorption was developed for the quarantine fumigation of fresh fruit based on the measurement of exposures and how they varied across the fumigation trials. The model describes how to manipulate the applied MB dose, the load factor, and the load geometry for different types of packaged fresh fruit so that the resultant exposure is adequate for insect control. C1 [Walse, Spencer S.; Bellamy, David E.; Obenland, David; Tebbets, Steve] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Myers, Scott W.] USDA, APHIS, CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Liu, Yong-Biao] USDA ARS, Crop Improvement & Protect Unit, Salinas, CA 93905 USA. [Simmons, Greg S.] USDA, APHIS, CPHST, Moss Landing, CA 95039 USA. RP Walse, SS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM spencer.walse@ars.usda.gov FU U.S. Department of Agriculture FX Jane Tebbets performed all calibration measurements for GC analysis of MB. We extend our gratitude to Alexander Cunningham, Kathleen Harding, Meghan Brumgard, Thomas Greene, Luis Mazuera, and Saben Kane for rearing and collecting insects for the trials. The U.S. Department of Agriculture financed this work. NR 36 TC 8 Z9 8 U1 1 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1155 EP 1163 DI 10.1603/EC11048 PG 9 WC Entomology SC Entomology GA 160IA UT WOS:000320111300012 PM 23865179 ER PT J AU Yokoyama, VY Cambron, SE AF Yokoyama, Victoria Y. Cambron, Sue E. TI Survival of Hessian Fly (Diptera: Cecidomyiidae) Puparia Exposed to Simulated Hay Harvest Conditions, Location and Windrow Drying in Washington and California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mayetiola destructor (Say); puparial survival; hay drying; quarantine control; systems approach ID MULTIPLE QUARANTINE TREATMENT; COMPRESSED HAY; WRAPPED BALES; LARGE-SIZE; JAPAN; EXPORT; DISINFESTATION; FUMIGATION; MORTALITY; EFFICACY AB Hessian fly, Mayetiola destructor (Say) (Diptera: Cecidomyiidae) puparia are of regulatory concern in exported hay, and drying after harvest was evaluated as a cultural control technique for bales shipped from the western states. In total 16,836; 31,122; and 48,051 puparia were tested under drying conditions in environmental chambers, open air on location, and hay windrows, respectively. Regression lines for percentage of total adults emerging from puparia exposed to simulated drying conditions for 1-7 d in environmental chambers was significant for 1 September, Kittitas Valley, WA; 3 June, East Columbia Basin, WA; 15 May and 15 July, San Joaquin Valley, CA; and 15 May, 20 July, and 15 September, Imperial Valley, CA. In open air drying on location for 1-7 d, total percentage of puparia surviving to adults for all exposure days was 0.4% for 18 June, Kittitas Valley; 1.2% for 15 May, San Joaquin Valley; and 0% for 16 July, Imperial Valley; and significantly different between controls and exposure durations. In hay windrow drying for 1D6 d, total percentage of puparia surviving to adults for all exposure days was 5.4% on 28 June and 24.2% on 7 September in timothy, Phleum pretense, in the Kittitas Valley; 3.8% on 28 June in timothy in the East Columbia Basin; 2.2% on 20 July in alfalfa, Medicago sativa, in the San Joaquin Valley; and 6.3% on 21 July in Sudan grass, Sorghum bicolor sudanensis, in the Imperial Valley. The number of puparia surviving to adults in open air drying and in windrows was significantly different between controls and exposure durations for all test dates and locations. Puparial survival in field tests was related to mild temperatures and high humidities. Hay drying with subsequent field baling, storage, and export bale compression is discussed in relation to a systems approach for quarantine control of Hessian fly in exported hay. C1 [Yokoyama, Victoria Y.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Cambron, Sue E.] Purdue Univ, USDA ARS, W Lafayette, IN 47907 USA. RP Yokoyama, VY (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM victoria.yokoyama@ars.usda.gov FU National Hay Association FX We thank Julie A. Gagnon, Sean K. Pelham, and Brandon A. Simons (USDA-ARS, SJVASC), and Hana A. Yokoyama-Hatch (University of California at Davis) for assistance with this project. We thank Daryl Petrey (USDA, APHIS, PPQ) and Benita Matheson (Washington State Department of Agriculture) in Ellensburg, WA, and Khaled M. Bali and Eric Natwick, (University of California Cooperative Extension) in Holtville, CA for their help with field work. We thank Rodney Van Orman and Ron T. Anderson (Anderson Hay & Grain) and Rollie Bernth (Ward Rugh) in Ellensburg, WA, and Gregory Nichol (Border Valley Trading) in Turlock, CA. This research was supported in part by The National Hay Association. NR 27 TC 3 Z9 3 U1 1 U2 10 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1164 EP 1172 DI 10.1603/EC12403 PG 9 WC Entomology SC Entomology GA 160IA UT WOS:000320111300013 PM 23865180 ER PT J AU Oliver, JB Ranger, CM Reding, ME Moyseenko, JJ Youssef, NN Bray, AM AF Oliver, Jason B. Ranger, Christopher M. Reding, Michael E. Moyseenko, James J. Youssef, Nadeer N. Bray, Alicia M. TI Preharvest Quarantine Treatments of Chlorantraniliprole, Clothianidin, and Imidacloprid-Based Insecticides for Control of Japanese Beetle (Coleoptera: Scarabaeidae) and Other Scarab Larvae in the Root Zone of Field-Grown Nursery Trees SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Popillia japonica; neonicotinoid; harmonization plan; scarab; insecticide ID WHITE GRUB COLEOPTERA; FIR CHRISTMAS TREES; POPILLIA-JAPONICA; SOUTHERN APPALACHIANS; MANAGEMENT; RESISTANCE; SOIL; CHLORPYRIFOS; OVIPOSITION; BIOLOGY AB Japanese beetle, Popillia japonica Newman (Coleoptera: Scarabaeidae), is an important quarantine pest of nurseries. Nursery plant movement from P. japonica-infested regions is regulated by the U.S. Domestic Japanese Beetle Harmonization Plan (DJHP), which classifies states by risk categories. Treatments for category 2 states include preharvest soil surface treatment of nursery plants grown in field soil using Discus SC, Marathon (1G or 60 WP), or Flagship (0.22G or 25 WG). In this study, Discus, Marathon 60 WP, or Flagship 0.22G DJHP standards were compared with labeled rates of non DDJHP-approved insecticides, including neonicotinoids clothianidin (Arena 50WDG), generic imidacloprid (Quali-Pro Imidacloprid 2 F T&O Insecticide, Mallet 2 F T&O Insecticide, and Lada 2 F Insecticide), and imidacloprid + bifenthrin (Allectus SC), as well as the anthranilic diamide, chlorantraniliprole (Acelepryn Insecticide). Arena provided 100% P. japonica control in May, June, and July over four test years, but had one larva recovered during August in two of those 4 yr. Acelepryn did not provide DJHP-acceptable P. japonica control. During July, Allectus provided 100% P. japonica control in three of four test years, but had four larvae in one test year. Other treatments tested only during July, which provided 100% P. japonica control, included Discus (five tests); Marathon, QualiPro, and Mallet (two tests); and Lada and Flagship (one test). Generic imidacloprid 2 F formulations were equivalent in P. japonica control to DJHP-approved insecticides. Insecticides generally performed poorly on other scarabs or curculionid larvae. The study supports Arena, Allectus, and generic imidacloprid 2 F products as suitable candidates for the DJHP. C1 [Oliver, Jason B.; Youssef, Nadeer N.; Bray, Alicia M.] Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, Mcminnville, TN 37110 USA. [Ranger, Christopher M.; Reding, Michael E.; Moyseenko, James J.] USDA ARS, Applicat Technol Res Unit, Hort Insects Res Lab, Wooster, OH 44691 USA. RP Oliver, JB (reprint author), Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, 472 Cadillac Lane, Mcminnville, TN 37110 USA. EM joliver@tnstate.edu FU FarmSaver; Syngenta Crop Protection; USDA-NIFA Evans-Allen; TSU-CAHNS FX We thank Jason Basham, Joshua Basham, Adam Blalock, Debbie Eskandarnia, Crystal Lemings, Joseph Lampley, Joshua Medley, Andrea Northcutt, and Samuel Patton (Tennessee State University College of Agriculture, Human, and Natural Sciences [TSU-CAHNS]) for study assistance. We thank Warren County High School summer interns Victoria Eschenbacher, Robert Eschenbacher, Elizabeth Oxley, Austin Shay, Jacob Weddington, Ben Wilson, and Lauren Wilson for assistance with the project. We thank Karla Addesso (TSU-CAHNS Chemical EcologyDEntomology) and Brett Seybert (TSU-CAHNS Technical Writer) for outside reviews of the manuscript. We thank Bayer Environmental Science, DuPont Professional Products, FarmSaver, OHP, Nufarm, Rotam North America, Syngenta Crop Protection, and Valent U. S. A. for supplying insecticide product. We also thank FarmSaver, Syngenta Crop Protection, USDA-NIFA Evans-Allen, and TSU-CAHNS for partial funding support. Mention of insecticides, companies, or other proprietary products does not imply any preferential treatment or endorsement by Tennessee State University or USDA-Agricultural Research Service. NR 45 TC 1 Z9 1 U1 3 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1190 EP 1199 DI 10.1603/EC13059 PG 10 WC Entomology SC Entomology GA 160IA UT WOS:000320111300016 PM 23865183 ER PT J AU Asiimwe, P Naranjo, SE Ellsworth, PC AF Asiimwe, Peter Naranjo, Steven E. Ellsworth, Peter C. TI Relative Influence of Plant Quality and Natural Enemies on the Seasonal Dynamics of Bemisia tabaci (Hemiptera: Aleyrodidae) in Cotton SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE whiteflies; arthropod predators; plant stress; cotton; biological control ID PHYTOPHAGOUS INSECT POPULATIONS; BOTTOM-UP FORCES; WATER-STRESS; TOP-DOWN; SPIDER-MITES; HOST PLANTS; HOMOPTERA ALEYRODIDAE; SPATIAL-DISTRIBUTION; SAMPLING PLANS; DROUGHT STRESS AB The abundance and distribution of insect herbivores is determined by, among other things, plant quality and natural enemies. These two factors vary temporally and spatially, subsequently affecting seasonal population dynamics. The relative influence of plant quality and natural enemies on the seasonal dynamics of Bemisia tabaci (Gennadius) was investigated in a 3-yr field study in cotton. Plant quality was manipulated through varying irrigation regimes: irrigations done at 20, 40, and 60% soil water depletions; and natural enemy densities were manipulated using broad spectrum insecticide applications that reduced their densities compared with unsprayed controls. In each year, densities of B. tabaci eggs, large nymphs and adults were consistently higher when natural enemy densities were reduced compared with when they were left unaltered, regardless of irrigation regime. In contrast, effects of plant quality on densities of all whitefly stages were weak and inconsistent. In addition, natural enemy densities and predator: prey ratios also were not generally affected by plant quality. Interactions between natural enemies and plant quality on whitefly dynamics were rare. In general, whitefly densities were elevated two-thirds of the time and increased two- to sixfold when natural enemy densities were reduced compared with plant quality effects which influenced whitefly densities about one-third of the time and were expressed inconsistently over the years. This indicates that natural enemies exert a comparatively greater influence on seasonal dynamics of B. tabaci in cotton than plant quality, as manipulated by differential irrigation. C1 [Asiimwe, Peter; Ellsworth, Peter C.] Univ Arizona, Dept Entomol, Maricopa Agr Ctr, Maricopa, AZ 85138 USA. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Asiimwe, P (reprint author), Univ Arizona, Dept Entomol, Maricopa Agr Ctr, 37860 West Smith Enke Rd, Maricopa, AZ 85138 USA. EM pasiimwe@email.arizona.edu OI Ellsworth, Peter/0000-0002-2485-0830 FU Cotton Incorporated; AZ Cotton Growers Association; The Cotton Foundations FX We thank Y. Carriere, G. Wang, R. Asiimwe and two anonymous reviewers for helpful comments on earlier drafts of this manuscript. Ed Martin provided guidance on establishing the irrigation regimes. M. Stefanek, V. Barkley, G. Castro, F. Bojorquez, B. Stuart, J. Trejo, A. Slade, L. Rodarte, and A. Sonoqui provided expert field and lab technical assistance. We also appreciate the funding support of Cotton Incorporated, AZ Cotton Growers Association, and The Cotton Foundations. NR 70 TC 4 Z9 4 U1 1 U2 30 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1260 EP 1273 DI 10.1603/EC12182 PG 14 WC Entomology SC Entomology GA 160IA UT WOS:000320111300024 PM 23865191 ER PT J AU Brewer, MJ Armstrong, JS Parker, RD AF Brewer, Michael J. Armstrong, J. Scott Parker, Roy D. TI Single and Multiple In-Season Measurements as Indicators of At-Harvest Cotton Boll Damage Caused by Verde Plant Bug (Hemiptera: Miridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE sucking bugs; plant bugs; pest monitoring; cotton boll damage ID SAMPLING PLANS; STINK; PENTATOMIDAE; INJURY; TRANSMISSION AB The ability to monitor verde plant bug, Creontiades signatus Distant (Hemiptera: Miridae), and the progression of cotton, Gossypium hirsutum L., boll responses to feeding and associated cotton boll rot provided opportunity to assess if single in-season measurements had value in evaluating at-harvest damage to bolls and if multiple in-season measurements enhanced their combined use. One in-season verde plant bug density measurement, three in-season plant injury measurements, and two at-harvest damage measurements were taken in 15 cotton fields in South Texas, 2010. Linear regression selected two measurements as potentially useful indicators of at-harvest damage: verde plant bug density (adjusted r(2) = 0.68; P = 0.0004) and internal boll injury of the carpel wall (adjusted r(2) = 0.72; P = 0.004). Considering use of multiple measurements, a stepwise multiple regression of the four in-season measurements selected a univariate model (verde plant bug density) using a 0.15 selection criterion (adjusted r(2) = 0.74; P = 0.0002) and a bivariate model (verde plant bug density-internal boll injury) using a 0.25 selection criterion (adjusted r(2) = 0.76; P = 0.0007) as indicators of at-harvest damage. In a validation using cultivar and water regime treatments experiencing low verde plant bug pressure in 2011 and 2012, the bivariate model performed better than models using verde plant bug density or internal boll injury separately. Overall, verde plant bug damaging cotton bolls exemplified the benefits of using multiple in-season measurements in pest monitoring programs, under the challenging situation when at-harvest damage results from a sequence of plant responses initiated by in-season insect feeding. C1 [Brewer, Michael J.] Texas A&M AgriLife Res, Corpus Christi, TX 78406 USA. [Brewer, Michael J.; Parker, Roy D.] Texas A&M AgriLife Res & Extens Ctr, Dept Entomol, Corpus Christi, TX 78406 USA. [Armstrong, J. Scott] USDA ARS, Stillwater, OK 74075 USA. [Parker, Roy D.] Texas A&M AgriLife Extens, Corpus Christi, TX 78406 USA. RP Brewer, MJ (reprint author), Texas A&M AgriLife Res, 10345 Hwy 44, Corpus Christi, TX 78406 USA. EM mjbrewer@ag.tamu.edu FU Texas State Support Committee [11-845]; Core Program of Cotton Inc. [11-952] FX We thank D. Anderson for comprehensive field research support, and M. Bloemer and J. Martinez, R. Villanueva, C. Farias, L. Pruter, H. Mensik, and N. Hammack for assistance in insect field sampling. Thanks to L. Hutchins, J. Norman, S. Hopkins, M. Treacy, J. Trolinger, and S. Biles for identifying sucking-bug infested fields, and we thank field owners (D. Mayo, M. Mutchler, R. Neiman, C. Neiman, D. Nunley, S. Simmons, L. Simmons, B. Simpson, T. Ulhorn, and M. Yeary) for allowing field access. The Texas A&M AgriLife Research and Extension Center, Corpus Christi, provided land and support to conduct the validations. R. Alaniz and C. Livingston assisted in harvest. Thanks to G. Medrano (USDA-ARS) for reviewing an earlier manuscript version. Partial financial support was provided by the Texas State Support Committee (11-845) and the Core Program (11-952) of Cotton Inc. NR 21 TC 2 Z9 2 U1 1 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1310 EP 1316 DI 10.1603/EC12422 PG 7 WC Entomology SC Entomology GA 160IA UT WOS:000320111300029 PM 23865196 ER PT J AU Lenz, M Kard, B Creffield, JW Evans, TA Brown, KS Freytag, ED Zhong, JH Lee, CY Yeoh, BH Yoshimura, T Tsunoda, K Vongkaluang, C Sornnuwat, Y Roland, TA de Santi, MP AF Lenz, Michael Kard, Brad Creffield, James W. Evans, Theodore A. Brown, Kenneth S. Freytag, Edward D. Zhong, Jun-Hong Lee, Chow-Yang Yeoh, Boon-Hoi Yoshimura, Tsuyoshi Tsunoda, Kunio Vongkaluang, Charunee Sornnuwat, Yupaporn Roland, Ted A., Sr. de Santi, Marie Pommier TI Ability of Field Populations of Coptotermes spp., Reticulitermes flavipes, and Mastotermes darwiniensis (Isoptera: Rhinotermitidae; Mastotermitidae) to Damage Plastic Cable Sheathings SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE subterranean termites; Coptotermes; plastic cable sheathings; nylon 12; polyethylene ID SUBTERRANEAN TERMITE ATTACK; SOUTHEAST-ASIA; RESISTANCE; AUSTRALIA; ACINACIFORMIS; HAVILANDI; COVERINGS; MALAYSIA; CHINA AB A comparative field study was conducted to evaluate the ability of subterranean termites to damage a set of four different plastic materials (cable sheathings) exposed below- and above-ground. Eight pest species from six countries were included, viz., Coptotermes formosanus (Shiraki) in China, Japan, and the United States; Coptotermes gestroi (Wasmann) in Thailand and Malaysia; Coptotermes curvignathus (Holmgren) and Coptotermes kalshoveni (Kemner) in Malaysia; Coptotermes acinaciformis (Froggatt) with two forms of the species complex and Mastotermes darwiniensis (Froggatt) in Australia; and Reticulitermes flavipes (Kollar) in the United States. Termite species were separated into four tiers relative to decreasing ability to damage plastics. The first tier, most damaging, included C. acinaciformis, mound-building form, and M. darwiniensis, both from tropical Australia. The second tier included C. acinaciformis, tree-nesting form, from temperate Australia and C. kalshoveni from Southeast Asia. The third tier included C. curvignathus and C. gestroi from Southeast Asia and C. formosanus from China, Japan, and the United States, whereas the fourth tier included only R. flavipes, which caused no damage. A consequence of these results is that plastics considered resistant to termite damage in some locations will not be so in others because of differences in the termite fauna, for example, resistant plastics from the United States and Japan will require further testing in Southeast Asia and Australia. However, plastics considered resistant in Australia will be resistant in all other locations. C1 [Lenz, Michael] CSIRO Ecosyst Sci, Canberra, ACT 2601, Australia. [Kard, Brad] Oklahoma State Univ, Dept Entomol & Plant Pathol, Noble Res Ctr 127, Stillwater, OK 74078 USA. [Creffield, James W.] Onwood Entomol Pty Ltd, Mt Eliza, Vic 3930, Australia. [Evans, Theodore A.] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore. [Brown, Kenneth S.] BASF Corp, Res Triangle Pk, NC 27709 USA. [Freytag, Edward D.] City New Orleans Mosquito Termite & Rodent Contro, New Orleans, LA 70126 USA. [Zhong, Jun-Hong] Guangdong Entomol Inst, Guangzhou 510260, Guangdong, Peoples R China. [Lee, Chow-Yang] Univ Sains Malaysia, Urban Entomol Lab, Vector Control Res Unit, Sch Biol Sci, George Town 11800, Malaysia. [Yeoh, Boon-Hoi] Bentz Jaz Singapore Pte Ltd, Singapore 608586, Singapore. [Tsunoda, Kunio] Kyoto Univ, Res Inst Sustainable Humanosphere, Kyoto 6110011, Japan. [Vongkaluang, Charunee; Sornnuwat, Yupaporn] Royal Forest Dept, Bangkok 10900, Thailand. [Roland, Ted A., Sr.] USDA, Poplarville, MS 39470 USA. [de Santi, Marie Pommier] Kyoto Tech Ctr, Arkema Japan, Shimogyou Ku, Kyoto 6008815, Japan. RP Lenz, M (reprint author), CSIRO Ecosyst Sci, GPO Box 1700, Canberra, ACT 2601, Australia. EM michael.lenz@csiro.au RI Lee, Chow-Yang/C-7129-2009; Evans, Theodore/A-6020-2008 OI Lee, Chow-Yang/0000-0001-8279-4104; Evans, Theodore/0000-0002-0558-3036 NR 30 TC 3 Z9 3 U1 1 U2 10 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1395 EP 1403 DI 10.1603/EC12514 PG 9 WC Entomology SC Entomology GA 160IA UT WOS:000320111300040 PM 23865207 ER PT J AU Castle, SJ Prabhaker, N AF Castle, S. J. Prabhaker, N. TI Monitoring Changes in Bemisia tabaci (Hemiptera: Aleyrodidae) Susceptibility to Neonicotinoid Insecticides in Arizona and California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE systemic uptake bioassay; insecticide resistance; desert environment; host plant ID CROSS-RESISTANCE; B-BIOTYPE; SILVERLEAF WHITEFLIES; SPECIES COMPLEX; IMPERIAL-VALLEY; SWEET-POTATO; COTTON; IDENTIFICATION; MANAGEMENT; HOMOPTERA AB Bemisia tabaci (Gennadius) biotype B is a highly prolific and polyphagous whitefly that established in much of North America during the 1980s. Neonicotinoid insecticides have been fundamental in regaining control over outbreak populations of B. tabaci, but resistance threatens their sustainability. Susceptibility of B. tabaci in the southwestern United States to four neonicotinoid insecticides varied considerably across populations within each year over a 3 yr period. Using a variability ratio of highest LC50 to lowest LC50 in field-collected whitefly adults from Arizona and California, the ranges of LC(50)s across all tests within compounds were highest to imidacloprid and lowest to thiamethoxam. Patterns of susceptibility were similar among all four neonicotinoid insecticides, but the greater variability in responses to imidacloprid and significantly higher LC(50)s attained indicated higher resistance levels to imidacloprid in all field populations. Further evidence of differential toxicities of neonicotinoids was observed in multiple tests of dinotefuran against imidacloprid-resistant lab strains that yielded significant differences in the LC(50)s of dinotefuran and imidacloprid in simultaneous bioassays. To test the possibility that resistance expression in field-collected insects was sometimes masked by stressful conditions, field strains cultured in a greenhouse without insecticide exposure produced significantly higher LC(50)s to all neonicotinoids compared with LC(50)s attained directly from the field. In harsh climates such as the American southwest, resistance expression in field-collected test insects may be strongly influenced by environmental stresses such as high temperatures, overcrowding, and declining host plant quality. C1 [Castle, S. J.] USDA ARS, Maricopa, AZ 85138 USA. [Prabhaker, N.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Castle, SJ (reprint author), USDA ARS, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM steven.castle@ars.usda.gov NR 36 TC 9 Z9 10 U1 0 U2 44 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1404 EP 1413 DI 10.1603/EC12322 PG 10 WC Entomology SC Entomology GA 160IA UT WOS:000320111300041 PM 23865208 ER PT J AU Jackson, DM Harrison, HF AF Jackson, D. Michael Harrison, Howard F., Jr. TI Insect Resistance in Traditional and Heirloom Sweetpotato Varieties SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE plant resistance; Ipomoea; Diabrotica; Cylas formicarius; white grub ID BATATAS L. LAM.; GENETIC-RESOURCES CONSERVATION; COLEOPTERA-CHRYSOMELIDAE; SCARABAEIDAE; DIVERSITY; MARKERS; SWEETPOTATOES; ELATERIDAE; MANAGEMENT; WEEVIL AB Fifty-nine sweetpotato cultivars, including 16 heirlooms, 11 near-heirlooms (developed in the 1960s and 1970s), 19 cultivars from the 1980s, and 13 modern varieties (since 1990), were evaluated for resistance to soil insects in field experiments during 2010-2011 at the U. S. Department of Agriculture-Agriculture Research Service, U. S. Vegetable Laboratory (USDA-ARS, USVL), Charleston, SC. These experiments included two insect-susceptible control cultivars ('Beauregard' and 'SC1149-19') and four insect-resistant control cultivars ('Charleston Scarlet,' 'Regal,' 'Ruddy,' and 'Sumor') that were developed by the USDA-ARS, USVL sweetpotato breeding program. Sweetpotato genotypes differed significantly in resistance measured by the overall percentage of injured roots, WDS (Wireworm, Diabrotica, and Systena) index, the percentage of roots damaged by the sweetpotato weevil (Cylas formicarius F.), the percentage of roots damaged by the sweetpotato flea beetle (Chaetocnema confinis Crotch), and the percentage of roots damaged by white grub larvae (including Plectris aliena Chapin and Phyllophaga spp.). Twenty-three sweetpotato cultivars had a lower percentage of injured roots than the susceptible control genotype, SC1149-19, while 14 varieties had a lower percentage of injured roots than Beauregard, one of the leading commercial orange-fleshed cultivars in the United States. Over the 2-yr period, Ruddy (7.6%) had the lowest percentage of injured roots and 'Carolina Ruby' (84.6%) the highest percentage of injured roots. Carolina Ruby (1.07) also had the highest WDS index, but 15 genotypes had a significantly lower WDS index than either susceptible control, SC1149-19 (1.03) or Beauregard (0.82). Ruddy (0.07) and 'Murasaki-29' (0.09) had the lowest WDS indices. Forty-five genotypes had a significantly lower percentage infestation by flea beetles than SC1149-19 (12.3%), and the highest level of flea beetle infestation was for 'Bonita' (18.9%). The highest percent white grub infestation was for 'Caromex' (19.6%), however none of the genotypes had significantly less white grubs than the susceptible controls. The highest infestation of sweetpotato weevils was observed for SC1149-19 (17.9%), while 29 genotypes had significantly lower percentage of sweetpotato weevil infestation than SC1149-19. The moderate to high levels of resistance to soil insect pests exhibited by many of these traditional and heirloom cultivars may provide useful sources of germplasm for sweetpotato breeding programs. C1 [Jackson, D. Michael; Harrison, Howard F., Jr.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Jackson, DM (reprint author), USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. EM mike.jackson@ars.usda.gov NR 60 TC 3 Z9 3 U1 1 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1456 EP 1462 DI 10.1603/EC12396 PG 7 WC Entomology SC Entomology GA 160IA UT WOS:000320111300047 PM 23865214 ER PT J AU Bansal, R Mian, MAR Michel, AP AF Bansal, Raman Mian, M. A. R. Michel, Andy P. TI Identification of Novel Sources of Host Plant Resistance to Known Soybean Aphid Biotypes SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aphis glycines; soybean; biotype; host plant resistance ID SINGLE DOMINANT GENE; HEMIPTERA-APHIDIDAE; GENOTYPES; LINES; INHERITANCE; ANTIXENOSIS; MANAGEMENT; GERMPLASM; JACKSON AB While soybean cultivars with resistance to the soybean aphid (Aphis glycines Matsumura) have been commercially released, the presence of virulent biotypes capable of overcoming plant resistance threatens the durability of host plant resistance as a stable management tactic. Novel sources of host plant resistance are needed to combat rapid biotype evolution. In this study, we screened 1,061 soybean plant introductions (PIs) for resistance to three known biotypes of A. glycines. Based on a series of growth chamber and field screenings, we identified 11 PIs that showed resistance to biotype 1 of A. glycines. Among these 11 PIs, 7 PIs were resistant to biotype 2 and 5 PIs were resistant to biotype 3. Further, two PIs (PI 606390A from Vietnam and PI 340034 from South Korea) showed resistance to all three biotypes of A. glycines. We also identified 11 PIs that were potentially tolerant to A. glycines, illustrated by no adverse impact on plant quality because of A. glycines infestation. As resistant PIs identified in this study belong to maturity group II-IV, they can be readily crossed to early maturing cultivars adapted to north-central states of the United States, where A. glycines is a major pest. The genetic characterization of resistance in these PIs and incorporation of novel resistant genes into elite soybean cultivars will broaden the defense against multiple biotypes of A. glycines. C1 [Bansal, Raman; Mian, M. A. R.; Michel, Andy P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. R.] Ohio State Univ, Dept Hort & Crop Sci, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Mian, M. A. R.] USDA ARS, Corn & Soybean Res Unit, Wooster, OH 44691 USA. RP Michel, AP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM michel.70@osu.edu FU Ohio Soybean Council; North Central Soybean Research Program; Ohio Agricultural Research and Development Center, The Ohio State University FX We are grateful to Esther Peregrine for providing us the seeds of soybean PIs tested in this study. We thank Lucinda Wallace, Vandana Bansal, Wei Zhang, Jane Todd, and Tim Mendiola for their help during this study. Aphid populations of biotype 2 and biotype 3 were kindly provided by C. Hill, Laboratory for Soybean Disease Research, University of Illinois, and biotype 1 was provided by J. Reese, Department of Entomology, Kansas State University. Funding was provided by the Ohio Soybean Council, the North Central Soybean Research Program, and Ohio Agricultural Research and Development Center, The Ohio State University. NR 35 TC 7 Z9 7 U1 0 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1479 EP 1485 DI 10.1603/EC12453 PG 7 WC Entomology SC Entomology GA 160IA UT WOS:000320111300050 PM 23865217 ER PT J AU Fontenot, EA Arthur, FH Nechols, JR Langemeier, MR AF Fontenot, Emily A. Arthur, Frank H. Nechols, James R. Langemeier, Michael R. TI Economic Feasibility of Methoprene Applied as a Surface Treatment and as an Aerosol Alone and in Combination With Two Other Insecticides SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE methoprene; esfenvalerate; Plodia interpunctella Hubner; economic analysis ID PLODIA-INTERPUNCTELLA LEPIDOPTERA; POPULATION-GROWTH MODEL; PEST-MANAGEMENT; SIMULATE RESPONSE; AMBIENT AERATION; STORED POPCORN; COST-ANALYSIS; 5TH INSTARS; COLEOPTERA; EFFICACY AB Economic evaluations of integrated pest management strategies are becoming increasingly important as restrictions on conventional insecticides continue to become more stringent and chemical control costs rise. Aerosol treatments with insect growth regulators alone and in combination with conventional contact insecticides may be a feasible alternative to expensive and highly toxic fumigants such as methyl bromide for control of the Indianmeal moth (Plodia interpunctella (Hubner)). Average calculated mortality of Indianmeal moth eggs exposed to surface applied methoprene, aerosol methoprene alone and in combination with esfenvalerate and synergized pyrethrins is 55.0, 69.0, and 94.6%, respectively. Temperature effects on development time makes frequency and timing of insecticide applications very important as evidenced by simulations of population levels in response to a variety of treatment dates by diet, and become critical in situations where survival of Indianmeal moth is high. Using a measurement of risk that is equal to deviations below a target mortality goal (99%), we are able to optimize cost and frequency of application using simulated mortality data for each of the treatment strategies. Optimal timing of each insecticide treatment depends heavily on the rate of development by diet. This type of analysis helps pest control operators and managers by showing consequences of treatment scenarios in time and cost. C1 [Fontenot, Emily A.] FAO IAEA Agr & Biotechnol Lab, A-2444 Seibersdorf, Austria. [Fontenot, Emily A.; Nechols, James R.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Langemeier, Michael R.] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. RP Fontenot, EA (reprint author), FAO IAEA Agr & Biotechnol Lab, Reaktorstr 1, A-2444 Seibersdorf, Austria. EM e.a.fontenot@iaea.gov FU USDA/National Institute of Food and Agriculture Risk Avoidance and Mitigation Program Award [00-511-01-9674] FX We thank Brian Adam of Oklahoma State University for reviewing a draft of this manuscript before submission. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA) or by Kansas State University. The USDA is an equal opportunity provider and employer. This study was supported by the USDA/National Institute of Food and Agriculture Risk Avoidance and Mitigation Program Award No. 00-511-01-9674. This article is contribution Number 13-159-J from the Kansas Agricultural Experiment Station. NR 34 TC 1 Z9 1 U1 2 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1503 EP 1510 DI 10.1603/EC12470 PG 8 WC Entomology SC Entomology GA 160IA UT WOS:000320111300053 PM 23865220 ER PT J AU St Jean, G Egan, SP Yee, WL Feder, JL AF St Jean, Gilbert Egan, Scott P. Yee, Wee L. Feder, Jeffrey L. TI Genetic Identification of an Unknown Rhagoletis Fruit Fly (Diptera: Tephritidae) Infesting Chinese Crabapple: Implications for Apple Pest Management SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE apple maggot; western cherry fruit fly; Rhagoletis indifferens; microsatellites; Malus spectabilis ID HOST-PLANT USE; SYMPATRIC SPECIATION; MAGGOT FLY; MOLECULAR-IDENTIFICATION; MICROSATELLITE LOCI; POMONELLA DIPTERA; WASHINGTON-STATE; SPECIES COMPLEX; DIVERGENCE; DIFFERENTIATION AB The apple maggot, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a serious introduced quarantine pest in the apple (Malus spp.)-growing regions of central Washington and Oregon. In August 2011, seven fly larvae of unknown origin were discovered infesting fruit of an exotic Chinese crabapple, Malus spectabilis (Aiton) Borkhausen, in Kennewick, Benton County, WA. If confirmed, Chinese crabapple would have represented a new host for R. pomonella in Washington and triggered quarantine measures in a surrounding three-county region of the state. Here, we establish, based on five microsatellite loci, the identity of the crabapple-infesting larvae as the western cherry fruit fly, Rhagoletis indifferens Curran, representing a new host record for the fly. Morphological analysis of six flies reared to adulthood confirmed the genetic identification. The results demonstrate the utility of integrating rapid genetic identification methods with field surveys of economic pests, which decreased detection times by months, and avoided enacting costly quarantine measures that saved local and federal bodies >US$0.5 million in monitoring, inspection, and control costs. We discuss current ongoing efforts to develop rapid, accurate, and inexpensive on site DNA-based detection tools for R. pomonella that would have general applicability for the control of pest insects. C1 [St Jean, Gilbert; Egan, Scott P.; Feder, Jeffrey L.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Egan, Scott P.; Feder, Jeffrey L.] Univ Notre Dame, Notre Dame, IN 46556 USA. [Yee, Wee L.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Feder, Jeffrey L.] Univ Notre Dame, Environm Change Initiat, Notre Dame, IN 46556 USA. RP Feder, JL (reprint author), Univ Notre Dame, Dept Biol Sci, Galvin Life Sci Bldg, Notre Dame, IN 46556 USA. EM feder.2@nd.edu FU Clark County Washington 78th street Heritage Farm; Washington State University Research and Extension Unit, Vancouver; National Science Foundation; USDA; Washington Tree Fruit Research Commission FX We thank Tom Powell, Glen Hood, Tracy Arcella, Sheina Sim, and Greg Ragland for helpful discussions, insight, advice, and constructive criticism of the paper. We also thank Blair Wolfley, Doug Stienbarger, Terry Porter, the Clark County Washington 78th street Heritage Farm, and the Washington State University Research and Extension Unit, Vancouver, for support and assistance through the years. This work was supported in part by grants to J.L.F. from the National Science Foundation and the USDA and to W.L.Y. by the Washington Tree Fruit Research Commission. NR 32 TC 2 Z9 2 U1 0 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2013 VL 106 IS 3 BP 1511 EP 1515 DI 10.1603/EC12449 PG 5 WC Entomology SC Entomology GA 160IA UT WOS:000320111300054 PM 23865221 ER PT J AU Zielinski, WJ Baldwin, JA Truex, RL Tucker, JM Flebbe, PA AF Zielinski, William J. Baldwin, James A. Truex, Richard L. Tucker, Jody M. Flebbe, Patricia A. TI Estimating Trend in Occupancy for the Southern Sierra Fisher Martes pennanti Population SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE fisher; Martes pennanti; monitoring; occupancy; population estimation; Sierra Nevada; California ID HABITAT SUITABILITY; LANDSCAPE-SCALE; CALIFORNIA; MODELS; USA; MANAGEMENT; FORESTS; NEVADA; RISKS; RARE AB Carnivores are important elements of biodiversity, not only because of their role in transferring energy and nutrients, but also because they influence the structure of the communities where they occur. The fisher Martes pennanti is a mammalian carnivore that is associated with late-successional mixed forests in the Sierra Nevada in California, and is vulnerable to the effects of forest management. As a candidate for endangered species status, it is important to monitor its population to determine whether actions to conserve it are successful. We implemented a monitoring program to estimate change in occupancy of fishers across a 12,240-km(2) area in the southern Sierra Nevada. Sample units were about 4 km apart, consisting of six enclosed, baited track-plate stations, and aligned with the national Forest Inventory and Analysis grid. We report here the results of 8 y (2002-2009) of sampling of a core set of 223 sample units. We model the combined effects of probability of detection and occupancy to estimate occupancy, persistence rates, and trend in occupancy. In combined models, we evaluated four forms of detection probability (1-group and 2-group both constant and varying by year) and nine forms of probability of occupancy (differing primarily by how occupancy and persistence vary among years). The best-fitting model assumed constant probability of occupancy, constant persistence, and two detection groups (AIC weight = 0.707). This fit the data best for the entire study area as well as two of the three distinct geographic zones therein. The one zone with a trend parameter found no significant difference from zero for that parameter. This suggests that, over the 8-y period, that there was no trend or statistically significant variations in occupancy. The overall probability of occupancy, adjusted to account for uncertain detection, was 0.367 (SE = 0.033) and estimates were lowest in the southeastern zone (0.261) and highest in the southwestern zone (0.583). Constant and positive persistence values suggested that sample units rarely changed status from occupied to unoccupied or vice versa. The small population of fishers in the southern Sierra (probably <250 individuals) does not appear to be decreasing. However, given the habitat degradation that has occurred in forests of the region, we favor continued monitoring to determine whether fisher occupancy increases as land managers implement measures to restore conditions favorable to fishers. C1 [Zielinski, William J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. [Baldwin, James A.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA 94706 USA. [Truex, Richard L.] US Forest Serv, USDA, Golden, CO 80401 USA. [Tucker, Jody M.; Flebbe, Patricia A.] US Forest Serv, USDA, Vallejo, CA 94592 USA. RP Zielinski, WJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU U.S. Department of Agriculture Pacific Southwest Region; Pacific Southwest Station FX We thank the U.S. Department of Agriculture Pacific Southwest Region and Pacific Southwest Station for financial support, the Sequoia National Forest for their administrative support, especially J. Whitfield, and dozens of field technicians for their assistance. J. Werren provided assistance with the figures and R. Schlexer provided editorial assistance. This manuscript was improved by the comments we received from the Subject Editor and anonymous reviewers. NR 49 TC 11 Z9 11 U1 4 U2 48 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2013 VL 4 IS 1 BP 3 EP 19 DI 10.3996/012012-JFWM-002 PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 163AC UT WOS:000320306700001 ER PT J AU Hefley, TJ Hygnstrom, SE Gilsdorf, JM Clements, GM Clements, MJ Tyre, AJ Baasch, DM VerCauteren, KC AF Hefley, Trevor J. Hygnstrom, Scott E. Gilsdorf, Jason M. Clements, Gregory M. Clements, Myndi J. Tyre, Andrew J. Baasch, David M. VerCauteren, Kurt C. TI Effects of Deer Density and Land Use on Mass of White-Tailed Deer SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE biofuels; body condition; cropland; density dependence; DeSoto National Wildlife Refuge; grassland; whitetailed deer ID LINEAR MIXED MODELS; PHYSICAL CONDITION; POPULATION-DENSITY; PARAMETERS; PERFORMANCE; SELECTION; INDEXES; HERD; OHIO; AGE AB Local and regional land use changes, such as the expansion of cropland for cellulosic biofuels, and the population density of white-tailed deer can affect the health and body mass of white-tailed deer Odocoileus virginianus. We collected hunter-harvest data for 1,731 white-tailed deer from DeSoto National Wildlife Refuge in eastern Nebraska and western Iowa from 2003 to 2010. We used linear mixed-effects models and information theoretic methods to estimate effects of density of white-tailed deer and proportion of total landcover area converted from cropland to cool-or warm-season grassland on body mass of white-tailed deer. Density of white-tailed deer at DeSoto National Wildlife Refuge ranged from 36.5 to 50.6 deer/km(2), and the proportion of landcover at DeSoto National Wildlife Refuge that remained cropland each year ranged from 14.9 to 23.2%. Body mass was inversely related to population density (21.4 kg/5.5 deer/km(2)) and proportion of cropland (21.3 kg/3.1% conversion of total land area from cropland to grassland). We used auxiliary harvest data collected at Boyer Chute National Wildlife Refuge to validate our models and found our models performed well. We estimate densities of white-tailed deer must be reduced by 1.7 (SE = 0.6) deer/km(2) for every 1% change in total landcover from cropland to grassland in order for white-tailed deer to maintain body mass. Our results indicate increased harvest of white-tailed deer, resulting in a reduction in population density, may offset negative effects that a decline in the amount of available cropland could have on the body mass and health of white-tailed deer. C1 [Hefley, Trevor J.; Hygnstrom, Scott E.; Gilsdorf, Jason M.; Clements, Gregory M.; Clements, Myndi J.; Tyre, Andrew J.; Baasch, David M.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Hefley, Trevor J.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [VerCauteren, Kurt C.] USDA APHIS WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Hefley, TJ (reprint author), 234 Hardin Hall,3310 Holdrege St, Lincoln, NE 68583 USA. EM thefley@huskers.unl.edu FU University of Nebraska-Lincoln UCARE program; USDA-APHIS-WS-National Wildlife Research Center; U.S. Fish and Wildlife Service; Nebraska Game and Parks Commission; Safari Club International; Nebraska Bowhunters Association; Professional Bowhunters Association; Cabela's Incorporated; Nebraska Chapter of The Wildlife Society FX We thank the graduate students, undergraduate students, technicians, and others who assisted in managing the check stations, and the hunters who allowed us to sex, age, and weigh their deer as well as staff of DNWR, especially M. Ellis, G. Gage, L. Klimek, M. Sheets, and S. Van Riper. We thank E. Blankenship and C. Frost for help with analyses and J. Fischer, anonymous reviewers, and the Subject Editor for their constructive reviews. We would also like to thank University of Nebraska-Lincoln UCARE program, USDA-APHIS-WS-National Wildlife Research Center, U.S. Fish and Wildlife Service, Nebraska Game and Parks Commission, Safari Club International, Nebraska Bowhunters Association, Professional Bowhunters Association, Cabela's Incorporated, and the Nebraska Chapter of The Wildlife Society for providing funding and support for the research. NR 58 TC 2 Z9 2 U1 2 U2 42 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2013 VL 4 IS 1 BP 20 EP 32 DI 10.3996/022012-JFWM-015 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 163AC UT WOS:000320306700002 ER PT J AU Vyas, NB Hulse, CS Meteyer, CU Rice, CP AF Vyas, Nimish B. Hulse, Craig S. Meteyer, Carol U. Rice, Clifford P. TI Evidence of Songbird Intoxication From Rozol (R) Application at a Black-Tailed Prairie Dog Colony SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE anticoagulant; chlorophacinone; rodenticide; songbird ID ANTICOAGULANT RODENTICIDES; TOXICITY; WILDLIFE; DIPHACINONE; STRYCHNINE AB Concerns about avian poisonings from anticoagulant rodenticides have traditionally focused on secondary poisoning of raptors exposed by feeding on contaminated mammalian prey. However, ground foraging songbirds can be directly poisoned from operational applications of the anticoagulant rodenticide Rozol (R) (0.005% chlorophacinone, active ingredient) applied as a grain bait, at black-tailed prairie dog Cynomys ludovicianus colonies. A dead western meadowlark Sturnella neglecta recovered from the study prairie dog colony displayed hemorrhaging in brain and pectoral muscle tissue, and it contained chlorophacinone residue concentrations of 0.59 and 0.49 mu g/g (wet weight) in the liver and intestinal contents, respectively. Chlorophacinone residues from two Rozol-colored songbird droppings found at the study colony were 0.09 and 0.46 mu g/g (wet weight). The timing of the meadowlark mortality and the occurrence of discolored droppings show that songbird exposure and poisoning can occur weeks after a Rozol application. C1 [Vyas, Nimish B.; Hulse, Craig S.] US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville Lab, Beltsville, MD 20705 USA. [Meteyer, Carol U.] US Geol Survey, Reston, VA 20192 USA. [Rice, Clifford P.] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. RP Vyas, NB (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville Lab, BARC East Bldg 308,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM nvyas@usgs.gov NR 25 TC 2 Z9 2 U1 1 U2 17 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2013 VL 4 IS 1 BP 97 EP 103 DI 10.3996/052012-JFWM-042 PG 7 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 163AC UT WOS:000320306700009 ER PT J AU Piaggio, AJ Coghlan, BA Miscampbell, AE Arjo, WM Ransome, DB Ritland, CE AF Piaggio, Antoinette J. Coghlan, Brett A. Miscampbell, Allyson E. Arjo, Wendy M. Ransome, Douglas B. Ritland, Carol E. TI Molecular phylogeny of an ancient rodent family (Aplodontiidae) SO JOURNAL OF MAMMALOGY LA English DT Article DE Aplodontia; control region; cytochrome b; growth hormone receptor; mountain beaver; phylogeny; taxonomy ID MITOCHONDRIAL CYTOCHROME-B; GROUND-SQUIRRELS; MOUNTAIN BEAVER; DNA; SYSTEMATICS; SEQUENCES; MAMMALS; RUFA; SPERMOPHILUS; BIOGEOGRAPHY AB The family Aplodontiidae contains a single, monotypic extant genus, Aplodontia (mountain beaver), which was first described by Rafinesque in 1817. Phylogenetic studies have shown that it is the sister lineage to squirrels. Aplodontia rufa is endemic to the Pacific Northwest and ranges from central California to British Columbia, Canada. Currently, 7 described subspecies are recognized based on morphological taxonomic studies. In this study, mitochondrial and nuclear genes were sequenced to infer molecular phylogenies of A. rufa. One of the goals of this study was to use molecular data to test the current taxonomic hypothesis based on morphology. Another goal was to incorporate geographic information to elucidate distributions of major clades. Our results support the previously held subspecies designations based on morphological taxonomy, with 1 main exception: we determined that within A. rufa, the subspecies A. rufa rainieri and A. rufa rufa north of the Columbia River represent a single lineage and should revert to the name A. rufa olympica. Although we revised geographic boundaries for some groups (A. r. rufa, A. r. olympica, and A. r. pacifica), only the conservation status and management of A. r. olympica (previously 2 subspecies) in Canada may be affected. Our findings support the continued conservation efforts for the isolated and endangered lineages present in coastal California. C1 [Piaggio, Antoinette J.; Coghlan, Brett A.] US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Wildlife Genet Lab, Ft Collins, CO 80521 USA. [Coghlan, Brett A.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Miscampbell, Allyson E.; Ritland, Carol E.] Univ British Columbia, Genet Data Ctr, Dept Forest Sci, Vancouver, BC V6T 1Z4, Canada. [Arjo, Wendy M.] US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Olympia Field Stn, Olympia, WA 98512 USA. [Ransome, Douglas B.] British Columbia Inst Technol, Fish Wildlife & Recreat Program, Burnaby, BC V5G 3H2, Canada. RP Piaggio, AJ (reprint author), US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Wildlife Genet Lab, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM toni.j.piaggio@aphis.usda.gov FU United States Department of Agriculture, Wildlife Services, National Wildlife Research Center; Washington Forest Protection Association; Oregon Forest Industries Council FX We thank W. Zielinski, M. Schwartz, and K. Pilgrim of the United States Forest Service for their kindness, generosity, and collaboration. We also extend our gratitude to T. Best for helpful discussions on taxonomic designations. We also thank M. A. Neubaum and J. Figueroa; J. Jeffers of the Nevada Department of Wildlife; and D. Girman and D. Crocker from Sonoma State University for their assistance in this project, including, in some cases, donations of samples. We also acknowledge K. Hamm, B. Youst, D. Lancaster, F. Arnold, B. Covell, J. Carr, R. Best, E. Meister, J. Duvall, J. Yost, J. Schaberl, T. Kocket, T. Losli, P. Happe, G. Phelan, R. Gonzalez, M. Dykzeul, and E. Myers for their help in obtaining samples. Thanks to J. Fillon and K. Ritland for their invaluable assistances in haplotype submission and discernment, respectively. We appreciate the support of the sabbatical program of the United States Department of Agriculture, Wildlife Services, National Wildlife Research Center; the Washington Forest Protection Association; and Oregon Forest Industries Council. Finally, we acknowledge the helpful comments and guidance from an anonymous reviewer. NR 76 TC 3 Z9 3 U1 1 U2 33 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 J9 J MAMMAL JI J. Mammal. PD JUN PY 2013 VL 94 IS 3 BP 529 EP 543 DI 10.1644/12-MAMM-A-016.1 PG 15 WC Zoology SC Zoology GA 165JF UT WOS:000320479100001 ER PT J AU Pastiu, AI Gyorke, A Blaga, R Mircean, V Rosenthal, BM Cozma, V AF Pastiu, Anamaria Ioana Gyoerke, Adriana Blaga, Radu Mircean, Viorica Rosenthal, Benjamin Martin Cozma, Vasile TI In Romania, exposure to Toxoplasma gondii occurs twice as often in swine raised for familial consumption as in hunted wild boar, but occurs rarely, if ever, among fattening pigs raised in confinement SO PARASITOLOGY RESEARCH LA English DT Article ID CROSS-SECTIONAL SURVEY; RISK-FACTORS; NEOSPORA-CANINUM; SUS-SCROFA; SEROPREVALENCE; INFECTION; ANTIBODIES; FARMS AB A wide range of swine husbandry practices prevail in Romania: pork for human consumption is derived from hunting wild boar, from household rearing of small numbers of backyard pigs, and from indoor, industrial production of swine raised in confinement indoors. Romania thus represents an instructive place for evaluating the influence of animal management on the exposure risk of the zoonotic parasite, Toxoplasma gondii. The fact that many Romanians eat uncooked or undercooked pork, especially when raised for household consumption, elevates the public health imperative to understand these risks. The aim of the study, therefore, was to evaluate the seroprevalence of T. gondii in pigs and wild boars from Romania. During 2008-2010, we collected 3,595 serum samples from pigs (fattening pigs, sows, backyard pigs) and 150 serum samples from wild boars. The sera were assayed by immunofluorescence antibody test (cutoff, 1:32) for antibodies against T. gondii. The overall seroprevalence of T. gondii infection was 23.1 % (829/3,595) in pigs and 16 % (24/150) in wild boars. The seroprevalence was significantly higher (p < 0.001) in backyard pigs (30.5 %; 783/2,564) than in sows (12.4 %; 46/371) or fattening pigs (none of the sera was positive). The management system (indoor pigs versus backyard pigs) represented the most important factor in the epidemiology of T. gondii infection. The proximity of backyard pigs to the definitive host of this parasite (cats), as well as their access to contaminated meat products, elevated their exposure risk well above that of pigs raised in confinement, and even above that of wild boars inhabiting sylvatic environments. C1 [Pastiu, Anamaria Ioana; Gyoerke, Adriana; Mircean, Viorica; Cozma, Vasile] Univ Agr Sci & Vet Med, Fac Vet Med, Parasitol & Parasit Dis Dept, Cluj Napoca 400372, Romania. [Blaga, Radu] Univ Paris Est, UMR BIPAR ANSES ENVA UPEC USC INRA, Ecole Natl Vet, F-94704 Maisons Alfort, France. [Rosenthal, Benjamin Martin] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Cozma, V (reprint author), Univ Agr Sci & Vet Med, Fac Vet Med, Parasitol & Parasit Dis Dept, 3-5 Manastur St, Cluj Napoca 400372, Romania. EM cozmavasile@yahoo.com OI Rosenthal, Benjamin/0000-0002-0224-3773 FU Ministry of Education, Research and Innovation of Romania through the Executive Unit [PNII PC 51-013/2007] FX This study was supported by the Ministry of Education, Research and Innovation of Romania through the Executive Unit for Financing Higher Education, Research, Development and Innovation from Romania, Grant PNII PC 51-013/2007. NR 25 TC 14 Z9 16 U1 2 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 J9 PARASITOL RES JI Parasitol. Res. PD JUN PY 2013 VL 112 IS 6 BP 2403 EP 2407 DI 10.1007/s00436-013-3353-z PG 5 WC Parasitology SC Parasitology GA 151MR UT WOS:000319465100035 PM 23455939 ER PT J AU Morris, LR Monaco, TA Leger, E Blank, R Sheley, R AF Morris, Lesley R. Monaco, Thomas A. Leger, Elizabeth Blank, Robert Sheley, Roger TI Cultivation legacies alter soil nutrients and differentially affect plant species performance nearly a century after abandonment SO PLANT ECOLOGY LA English DT Article DE Agricultural legacies; Legacy effects; Secondary succession; Ex-arable fields; Old fields; Bromus tectorum ID LAND-USE LEGACIES; BROMUS-TECTORUM L.; COMMUNITIES; VEGETATION; ECOSYSTEMS; RECOVERY; GROWTH; BIODIVERSITY; POPULATIONS; AMENDMENTS AB Cultivation legacies affect native vegetation in old fields of the Great Basin, USA for nearly a century after these fields are abandoned. We hypothesized that cultivation lowered soil nutrients and that this legacy would differentially impact plant performance of four representative Great Basin species. To test these hypotheses, we compared soil nutrients (C, N, P, K, Mg and Ca) between two formerly cultivated and adjacent noncultivated sites in two soil series. We then compared the plant growth and foliar nutrient content of an exotic grass (Bromus tectorum L.), two native grasses (Elymus elymoides [Raf.] Swezey and Achnatherum hymenoides [Roem. and Schult.] Barkworth), and a native forb (Sphaeralcea grossulariifolia [Hook. and Arn.] Rydb) grown in these soils in the greenhouse and in the field. Only one sampling site had reduced soil nutrients associated with cultivation legacies, where most of the negative effects on plant performance were found. E. elymoides appeared to be less affected by cultivation legacies than did A. hymenoides, which had a reduced survivorship and 20 % less above-ground biomass in cultivated soils. No species, including B. tectorum, were favored by cultivation. Our findings suggest that cultivation legacies can affect plant performance of different species in different ways and that altered soil nutrients may interact with other abiotic and biotic cultivation legacies in complex ways. C1 [Morris, Lesley R.; Monaco, Thomas A.] Utah State Univ, Forage & Range Res Lab, USDA ARS, Logan, UT 84322 USA. [Leger, Elizabeth] Univ Nevada, Reno, NV 89557 USA. [Blank, Robert] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Sheley, Roger] USDA ARS, Burns, OR 97720 USA. RP Morris, LR (reprint author), Utah State Univ, Forage & Range Res Lab, USDA ARS, 696 North 1100 East, Logan, UT 84322 USA. EM LesleyRMorris@gmail.com FU United States Department of Agriculture, Agricultural Research Service area-wide Ecologically Based Invasive Plant Management project FX This research was funded by the United States Department of Agriculture, Agricultural Research Service area-wide Ecologically Based Invasive Plant Management project. We would like to thank Justin Williams and Josh Leffler for work in the field and Justin Williams, Sandra Li, and Tye Morgan for greenhouse and laboratory assistance. Special thanks to the Salt Lake Field Office of the United States Department of Interior, Bureau of Land Management and the private property owners in Utah. We are also grateful to Jeremy James, Josh Leffler, and two anonymous reviewers for suggestions on an earlier draft of this manuscript. NR 41 TC 1 Z9 2 U1 3 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD JUN PY 2013 VL 214 IS 6 BP 831 EP 844 DI 10.1007/s11258-013-0212-z PG 14 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 163IN UT WOS:000320329600004 ER PT J AU Wolf, RA Girouard, N Xu, SH Meredith, JC Shofner, ML Cross, L Mintz, E Schueneman, GT AF Wolf, Rory A. Girouard, Natalie Xu, Shanhong Meredith, J. Carson Shofner, Meisha L. Cross, Lionel Mintz, Eric Schueneman, Gregory T. TI Adhesion Improvements of Nanocellulose Composite Interfaces SO PLASTICS ENGINEERING LA English DT Article ID BIODEGRADABLE POLYMERS; NANOCOMPOSITES; BLENDS C1 [Girouard, Natalie; Xu, Shanhong; Meredith, J. Carson] Georgia Inst Technol, Sch Chem & Bimol Engn, Atlanta, GA 30332 USA. [Shofner, Meisha L.] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. [Cross, Lionel; Mintz, Eric] Clark Atlanta Univ, Dept Chem, Atlanta, GA 30314 USA. [Schueneman, Gregory T.] US Forest Serv, Performance Enhanced Biopolymers Forest Prod Lab, USDA, Morgantown, WV USA. RI Meredith, Carson/B-3323-2009 OI Meredith, Carson/0000-0003-2519-5003 FU U.S. Forest Service [10-JV-11111104-066] FX The authors would like to gratefully acknowledge partial support of this work by U.S. Forest Service Grant 10-JV-11111104-066. NR 13 TC 1 Z9 1 U1 0 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0091-9578 J9 PLAST ENG JI Plast. Eng. PD JUN PY 2013 VL 69 IS 6 BP 32 EP 37 PG 6 WC Engineering, Chemical; Materials Science, Multidisciplinary SC Engineering; Materials Science GA 167OA UT WOS:000320640100006 ER PT J AU Turner, M Lenhart, S Rosenthal, B Zhao, XP AF Turner, Matthew Lenhart, Suzanne Rosenthal, Benjamin Zhao, Xiaopeng TI Modeling effective transmission pathways and control of the world's most successful parasite SO THEORETICAL POPULATION BIOLOGY LA English DT Article DE Toxoplasma gondii; Epidemic model; Transmission mechanisms; Differential equations ID TOXOPLASMA-GONDII; CAT POPULATIONS; DYNAMICS; DISEASE AB Toxoplasma gondii (T. gondii) is a single-celled, intracellular protozoan responsible for the disease toxoplasmosis. The parasite is prevalent worldwide, and it infects all warm-blooded vertebrates. Consumption of meats in which this parasite has encysted confers risk of infection to people and other animals, as does ingestion of water or foods contaminated with environmentally resistant oocysts excreted by cats. Vertical transmission (from mother to offspring) is also possible, leading to disease risk and contributing additional means of ensuring perpetuation of transmission. In this work, we adopt a differential equation model to investigate the effective transmission pathways of T. gondii, as well as potential control mechanisms. Detailed analyses are carried out to examine the significance of transmission routes, virulence, vertical transmission, parasite-induced changes in host behavior, and controls based on vaccination and harvesting. Modeling and analysis efforts may shed insights into understanding the complex life cycle of T. gondii. (c) 2013 Elsevier Inc. All rights reserved. C1 [Turner, Matthew; Zhao, Xiaopeng] Univ Tennessee, Dept Mech Aerosp & Biomed Engn, Knoxville, TN 37996 USA. [Turner, Matthew; Lenhart, Suzanne] Univ Tennessee, Dept Math, Knoxville, TN 37996 USA. [Lenhart, Suzanne; Zhao, Xiaopeng] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN USA. [Rosenthal, Benjamin] USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Zhao, XP (reprint author), Univ Tennessee, Dept Mech Aerosp & Biomed Engn, Knoxville, TN 37996 USA. EM xzhao9@utk.edu RI Zhao, Xiaopeng/A-4419-2008; OI Zhao, Xiaopeng/0000-0003-1207-5379; Rosenthal, Benjamin/0000-0002-0224-3773 FU National Science Foundation; US Department of Homeland Security; US Department of Agriculture through NSF [EF-0832858]; University of Tennessee, Knoxville; NSF [CMMI-0845753] FX This work was assisted through participation in Multiscale Modeling of the Life Cycle of Toxoplasma gondii Working Group at the National Institute for Mathematical and Biological Synthesis (NIMBioS), sponsored by the National Science Foundation, the US Department of Homeland Security, and the US Department of Agriculture through NSF Award EF-0832858, with additional support from The University of Tennessee, Knoxville. This work was in part supported by the NSF under grant CMMI-0845753. NR 17 TC 5 Z9 5 U1 3 U2 41 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0040-5809 J9 THEOR POPUL BIOL JI Theor. Popul. Biol. PD JUN PY 2013 VL 86 BP 50 EP 61 DI 10.1016/j.tpb.2013.04.001 PG 12 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 169AY UT WOS:000320750500006 PM 23624067 ER PT J AU Alston, JM Okrent, AM Rickard, BJ AF Alston, Julian M. Okrent, Abigail M. Rickard, Bradley J. TI Impact of agricultural policies on caloric consumption SO TRENDS IN ENDOCRINOLOGY AND METABOLISM LA English DT Editorial Material DE US farm subsidies; food consumption ID OBESITY AB In this article we use an economic model to link markets for agricultural commodities to food-product markets, and to trace the effects of agricultural policies on prices of food products and, consequently, on food consumption and calorie intake. Contrary to common claims, US agricultural policies have had generally modest and mixed effects on prices and quantities of farm commodities, with negligible effects on the prices paid by consumers for food, and thus a negligible influence on dietary patterns and obesity. C1 [Alston, Julian M.] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA 95616 USA. [Okrent, Abigail M.] USDA, ERS, Washington, DC 20009 USA. [Rickard, Bradley J.] Cornell Univ, Charles H Dyson Sch Appl Econ & Management, Ithaca, NY 14853 USA. RP Rickard, BJ (reprint author), Cornell Univ, Charles H Dyson Sch Appl Econ & Management, Ithaca, NY 14853 USA. EM bjr83@cornell.edu NR 15 TC 0 Z9 0 U1 2 U2 13 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1043-2760 J9 TRENDS ENDOCRIN MET JI Trends Endocrinol. Metab. PD JUN PY 2013 VL 24 IS 6 BP 269 EP 271 DI 10.1016/j.tem.2012.12.004 PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 168YC UT WOS:000320742900001 PM 23336994 ER PT J AU Murray, RE Bantchev, GB Dunn, RO Ascherl, KL Doll, KM AF Murray, Rex E. Bantchev, Grigor B. Dunn, Robert O. Ascherl, Kim L. Doll, Kenneth M. TI Thioether-Functionalized Vegetable Oils: Metal-Absorbing Biobased Ligands SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Remediation; Thiolated oil; Vegetable oil derivative; Metal Chelate; Vegetable oil ligand; Thioether sulfide; Ligand ID PENDANT CROWN THIOETHERS; RENEWABLE RAW-MATERIALS; SOYBEAN OIL; CHEMISTRY; EXTRACTANTS; DERIVATIVES; BUTANETHIOL; POLYMER; HEAVY; FATS AB Vegetable oils containing thioether groups have been synthesized and used to effectively remove heavy metal ions from aqueous solution. The use of thioether-functionalized corn oil (TF-corn oil) and thioether-functionalized canola oil (TF-canola oil) were both effective in the extraction of Ag+ from a 600 ppm aqueous silver nitrate solution. The TF-corn oil reduced the observed silver in solution to a level below the detection limit, whereas the TF-canola oil was only able to remove slightly over 50% of the concentration. A study of the absorption rate is also reported, where both the TF-corn oil and the TF-canola oil removed the Ag+ rapidly, at nearly the same rate. Preliminary results suggest that the efficacy and capacity of silver removal relates directly to the fatty acid composition of the oil precursor. C1 [Murray, Rex E.; Bantchev, Grigor B.; Dunn, Robert O.; Ascherl, Kim L.; Doll, Kenneth M.] USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, Peoria, IL 61604 USA. RP Murray, RE (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Rex.Murray@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 22 TC 2 Z9 2 U1 0 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD JUN PY 2013 VL 1 IS 6 BP 562 EP 565 DI 10.1021/sc300164y PG 4 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA 159AD UT WOS:000320015500002 ER PT J AU Rai, DK Schafer, EA Singh, K McIntosh, MA Sarafianos, SG Rieder, E AF Rai, Devendra K. Schafer, Elizabeth A. Singh, Kamalendra McIntosh, Mark A. Sarafianos, Stefan G. Rieder, Elizabeth TI Repeated exposure to 5D9, an inhibitor of 3D polymerase, effectively limits the replication of foot-and-mouth disease virus in host cells SO ANTIVIRAL RESEARCH LA English DT Article DE Foot-and-mouth disease virus (FMDV); 5D9 compound; 3D polymerase inhibitor; FMDV therapeutic treatment; Antiviral ID DEPENDENT RNA-POLYMERASE; DRUG-RESISTANCE; FIDELITY; INITIATION; RIBAVIRIN; COMPLEX; PROTEIN; MECHANISMS; EVOLUTION; ANIMALS AB Foot-and-mouth disease (FMD) is a highly contagious disease of livestock caused by a highly variable RNA virus (FMDV) that has seven serotypes and more than sixty subtypes. Both prophylactic and post-infection means of controlling the disease outbreak, including universally applicable vaccines and emergency response measures such as therapeutic treatments, are on high demand. In this study, we analyzed the long-term exposure outcome to a previously identified inhibitor of 3D polymerase (FMDV 3Dpol) for controlling FMDV infection and for the selection of resistance mutants. The results showed that no escape mutant viruses were isolated from FMDV A24 Cruzeiro infections in cell culture treated with gradually increasing concentrations of the antiviral compound 5D9 (4-chloro-N'-thieno, [2,3-d]pyrimidin-4-ylbenzenesulfonohydrazide) over ten passages. Biochemical and plaque assays revealed that when 5D9 was used at concentrations within a non-toxic range in cells, it drove the virus to undetectable levels at passage eight to ten. This is in contrast with observations made on parallel control (untreated) passages exhibiting fully viable and stable virus progenies. Collectively, the results demonstrated that under the experimental conditions, treatment with 5D9 does not confer a resistant phenotype and the virus is unable to evade the antiviral effect of the inhibitor. Further efforts using quantitative structure-property relationship (QSPR) based modifications of the 5D9 compound may result in the successful development of an effective in vivo antiviral drug targeting FMDV. (C) 2013 Elsevier B.V. All rights reserved. C1 [Rai, Devendra K.; Schafer, Elizabeth A.; Rieder, Elizabeth] USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, Greenport, NY 11944 USA. [Singh, Kamalendra; Sarafianos, Stefan G.] Univ Missouri, Sch Med, Christopher Bond Life Sci Ctr, Columbia, MO USA. [Singh, Kamalendra; McIntosh, Mark A.; Sarafianos, Stefan G.] Univ Missouri, Sch Med, Dept Mol Microbiol & Immunol, Columbia, MO 65212 USA. [Sarafianos, Stefan G.] Univ Missouri, Dept Biochem, Columbia, MO USA. RP Sarafianos, SG (reprint author), USDA ARS NAA, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM sarafianoss@missouri.edu; elizabeth.rieder@ars.usda.gov RI Rai, Devendra/J-3857-2013; OI Rai, Devendra/0000-0001-9035-685X; Sarafianos, Stefan G/0000-0002-5840-154X FU CRIS project [1940-32000-057-00D]; Agricultural Research Service (ARS), U.S. Department of Agriculture; NIH [AI076119, AI099284, AI100890]; Plum Island Animal Disease Research Participation Program; [USDA-ARS-58-1940-5-519]; [USDA-ARS-58-1940-8-868] FX This research was supported in part by CRIS project no. 1940-32000-057-00D, Agricultural Research Service (ARS), U.S. Department of Agriculture (Elizabeth Rieder) and USDA-ARS-58-1940-5-519. USDA-ARS-58-1940-8-868; NIH grants AI076119, AI099284, and AI100890 (Stefan G. Sarafianos). Dr D. Rai is the recipient of a fellowship by the Plum Island Animal Disease Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Department of Agriculture. We thank A. Clavijo (National Centre for Foreign Animal Disease, 1015 Arlington Street, Winnipeg, Manitoba, Canada) for the gift of monoclonal antibodies targeting the FMDV 3D polymerase protein. NR 50 TC 5 Z9 5 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-3542 J9 ANTIVIR RES JI Antiviral Res. PD JUN PY 2013 VL 98 IS 3 BP 380 EP 385 DI 10.1016/j.antiviral.2013.03.022 PG 6 WC Pharmacology & Pharmacy; Virology SC Pharmacology & Pharmacy; Virology GA 162WJ UT WOS:000320296400004 PM 23578728 ER PT J AU Yao, Y Gao, B Chen, JJ Zhang, M Inyang, M Li, YC Alva, A Yang, LY AF Yao, Ying Gao, Bin Chen, Jianjun Zhang, Ming Inyang, Mandu Li, Yuncong Alva, Ashok Yang, Liuyan TI Engineered carbon (biochar) prepared by direct pyrolysis of Mg-accumulated tomato tissues: Characterization and phosphate removal potential SO BIORESOURCE TECHNOLOGY LA English DT Article DE Engineered carbon; Biochar; Magnesium; Nanocomposites; Phosphate ID SUGAR-BEET TAILINGS; ARSENIC REMOVAL; MAGNETIC BIOCHAR; AQUEOUS-SOLUTION; HEAVY-METALS; SORPTION; NITRATE; ADSORBENTS; ADSORPTION; PLANTS AB An innovative method was developed to produce engineered biochar from magnesium (Mg) enriched tomato tissues through slow pyrolysis in a N-2 environment. Tomato plants treated with 25 mM Mg accumulated much higher level of Mg in tissue, indicating Mg can be substantially enriched in tomato plants, and pyrolysis process further concentrated Mg in the engineered biochar (8.8% Mg). The resulting Mg-biochar composites (MgEC) showed better sorption ability to phosphate (P) in aqueous solutions compared to the other four tomato leaves biochars. Statistical analysis showed a strong and significant correlation between P removal rate and biochar Mg content (R-2 = 0.78, and p < 0.001), indicating the enriched Mg in the engineered biochar is the main factor controlling its P removal ability. SEM-EDX, XRD and XPS analyses showed that nanoscale Mg(OH)(2) and MgO particles were presented on the surface of MgEC, which serve as the main adsorption sites for aqueous P. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Yao, Ying; Gao, Bin; Zhang, Ming; Inyang, Mandu] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Chen, Jianjun] Univ Florida, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Li, Yuncong] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. [Alva, Ashok] USDA ARS, Prosser, WA 99350 USA. [Yang, Liuyan] Nanjing Univ, Sch Environm, Nanjing 210046, Jiangsu, Peoples R China. RP Gao, B (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM bg55@ufl.edu RI Gao, Bin/B-8027-2010 OI Gao, Bin/0000-0003-3769-0191 NR 29 TC 47 Z9 49 U1 18 U2 213 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUN PY 2013 VL 138 BP 8 EP 13 DI 10.1016/j.biortech.2013.03.057 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 162WL UT WOS:000320296600002 PM 23612156 ER PT J AU Brantley, S Ford, CR Vose, JM AF Brantley, Steven Ford, Chelcy R. Vose, James M. TI Future species composition will affect forest water use after loss of eastern hemlock from southern Appalachian forests SO ECOLOGICAL APPLICATIONS LA English DT Article DE Adelges tsugae; Betula lenta; eastern hemlock; evapotranspiration; hemlock woolly adelgid; invasive species; Jarvis model; Rhododendron maximum; sap flux; Tsuga canadensis; vapor pressure deficit; water use ID WOOLLY ADELGID INFESTATION; TSUGA-CANADENSIS; SAP-FLOW; RHODODENDRON-MAXIMUM; STOMATAL CONDUCTANCE; BETULA-LENTA; NEW-ENGLAND; CLIMATE; TRANSPIRATION; DYNAMICS AB Infestation of eastern hemlock (Tsuga canadensis (L.) Carr.) with hemlock woolly adelgid (HWA, Adelges tsugae) has caused widespread mortality of this key canopy species throughout much of the southern Appalachian Mountains in the past decade. Because eastern hemlock is heavily concentrated in riparian habitats, maintains a dense canopy, and has an evergreen leaf habit, its loss is expected to have a major impact on forest processes, including transpiration (E-t). Our goal was to estimate changes in stand-level E-t since HWA infestation, and predict future effects of forest regeneration on forest E-t in declining eastern hemlock stands where hemlock represented 50-60% of forest basal area. We used a combination of community surveys, sap flux measurements, and empirical models relating sap flux-scaled leaf-level transpiration (E-L) to climate to estimate the change in E-t after hemlock mortality and forecast how forest E-t will change in the future in response to eastern hemlock loss. From 2004 to 2011, eastern hemlock mortality reduced annual forest E-t by 22% and reduced winter E-t by 74%. As hemlock mortality increased, growth of deciduous tree species-especially sweet birch (Betula lenta L.), red maple (Acer rubrum L.), yellow poplar (Liriodendron tulipifera L.), and the evergreen understory shrub rosebay rhododendron (Rhododendron maximum L.)-also increased, and these species will probably dominate post-hemlock riparian forests. All of these species have higher daytime E-L rates than hemlock, and replacement of hemlock with species that have less conservative transpiration rates will result in rapid recovery of annual stand E-t. Further, we predict that annual stand E-t will eventually surpass E-t levels observed before hemlock was infested with HWA. This long-term increase in forest E-t may eventually reduce stream discharge, especially during the growing season. However, the dominance of deciduous species in the canopy will result in a permanent reduction in winter E-t and possible increase in winter stream discharge. The effects of hemlock die-off and replacement with deciduous species will have a significant impact on the hydrologic flux of forest transpiration, especially in winter. These results highlight the impact that invasive species can have on landscape-level ecosystem fluxes. C1 [Brantley, Steven] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Brantley, Steven; Ford, Chelcy R.; Vose, James M.] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. RP Brantley, S (reprint author), Coweeta Hydrol Lab, 3160 Coweeta Lab Rd, Otto, NC 28763 USA. EM sbrantle@umn.edu OI Miniat, Chelcy/0000-0002-3266-9783 FU USDA Forest Service, Southern Research Station; NSF [DEB0218001, DEB0823293] FX This study was supported by the USDA Forest Service, Southern Research Station and by NSF grants DEB0218001 and DEB0823293 to the Coweeta LTER program at the University of Georgia. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the authors and do not necessarily reflect the views of the National Science Foundation or the University of Georgia. We are grateful to Stan Wullschleger and David Orwig for providing helpful comments on a previous version of this manuscript. We acknowledge the support of many individuals, past and present, as well as the long-term climate and hydrologic data network at Coweeta Hydrologic Lab, especially K. Bower, J. Davis, B. Kloeppel, S. Laseter, J. P. Love, R. McCollum, C. Marshall, N. Muldoon, J. Meador, and G. Zausen for fieldwork and climate data collection and processing. NR 61 TC 15 Z9 17 U1 5 U2 80 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2013 VL 23 IS 4 BP 777 EP 790 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 160GE UT WOS:000320105100008 PM 23865229 ER PT J AU Augustine, DJ Springer, TL AF Augustine, David J. Springer, Tim L. TI Competition and facilitation between a native and a domestic herbivore: trade-offs between forage quantity and quality SO ECOLOGICAL APPLICATIONS LA English DT Article DE black-tailed prairie dog; Cynomys ludovicianus; digestibility; forage intake rate; grazing; nitrogen; northern mixed prairie; semiarid grassland; shortgrass steppe; spatiotemporal variation; western Great Plains, USA ID BLACK-TAILED PRAIRIE; RESOURCE COMPETITION; DIETARY OVERLAP; DOG; CATTLE; LIVESTOCK; PLANT; DYNAMICS; PLAINS; SUPPLEMENTATION AB Potential competition between native and domestic herbivores is a major consideration influencing the management and conservation of native herbivores in rangeland ecosystems. In grasslands of the North American Great Plains, black-tailed prairie dogs (Cynomys ludovicianus) are widely viewed as competitors with cattle but are also important for biodiversity conservation due to their role in creating habitat for other native species. We examined spatiotemporal variation in prairie dog effects on growing-season forage quality and quantity using measurements from three colony complexes in Colorado and South Dakota and from a previous study of a fourth complex in Montana. At two complexes experiencing below-average precipitation, forage availability both on and off colonies was so low (12-54 g/m(2)) that daily forage intake rates of cattle were likely constrained by instantaneous intake rates and daily foraging time. Under these dry conditions, prairie dogs (1) substantially reduced forage availability, thus further limiting cattle daily intake rates, and (2) had either no or a small positive effect on forage digestibility. Under such conditions, prairie dogs are likely to compete with cattle in direct proportion to their abundance. For two complexes experiencing above-average precipitation, forage quantity on and off colonies (77-208 g/m(2)) was sufficient for daily forage intake of cattle to be limited by digestion rather than instantaneous forage intake. At one complex where prairie dogs enhanced forage digestibility and [N] while having no effect on forage quantity, prairie dogs are predicted to facilitate cattle mass gains regardless of prairie dog abundance. At the second complex where prairie dogs enhanced digestibility and [N] but reduced forage quantity, effects on cattle can vary from competition to facilitation depending on prairie dog abundance. Our findings show that the high spatiotemporal variation in vegetation dynamics characteristic of semiarid grasslands is paralleled by variability in the magnitude of competition between native and domestic grazers. Competitive interactions evident during dry periods may be partially or wholly offset by facilitation during periods when forage digestibility is enhanced and forage quantity does not limit the daily intake rate of cattle. C1 [Augustine, David J.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Springer, Tim L.] ARS, Southern Plains Range Res Stn, USDA, Woodward, OK 73801 USA. RP Augustine, DJ (reprint author), ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM David.Augustine@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2008-55101-19208]; USDA-Agricultural Research Service FX We thank T. Odell, P. McCusker, S. Podolak, E. Klarquist, R. Moore, and D. Willis for their dedicated assistance in the field and laboratory, and J. McLaughlin for laboratory analyses of forage quality. We thank J. Derner, D. Milchunas, and S. Guenther for helpful discussions of plant species palatability and approaches to examining the foraging environment for cattle. Funding for this study was provided by Agriculture and Food Research Initiative Competitive Grant no. 2008-55101-19208 from the USDA National Institute of Food and Agriculture, and by USDA-Agricultural Research Service. Mention of trade names is for information only and does not imply an endorsement. NR 65 TC 10 Z9 11 U1 5 U2 86 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2013 VL 23 IS 4 BP 850 EP 863 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 160GE UT WOS:000320105100014 PM 23865235 ER PT J AU Lundgren, JG Saska, P Honek, A AF Lundgren, Jonathan G. Saska, Pavel Honek, Alois TI Molecular approach to describing a seed-based food web: the post-dispersal granivore community of an invasive plant SO ECOLOGY AND EVOLUTION LA English DT Article DE Granivore; gut analysis; seed predation; Taraxacum officinale; trophic linkages ID DANDELION TARAXACUM-OFFICINALE; ORGANIC CEREAL FIELDS; WINTER-WHEAT; PREDATION; INVERTEBRATES; DISPERSAL; BEHAVIOR; ANTS; AVAILABILITY; CONSUMPTION AB Communities of post-dispersal granivores can shape the density and dispersion of exotic plants and invasive weeds, yet plant ecologists have a limited perception of the relative trophic linkages between a seed species and members of its granivore community. Dandelion seeds marked with Rabbit IgG were disseminated into replicated plots in the recipient habitat (South Dakota) and the native range (Czech Republic). Arthropods were collected in pitfall traps, and their guts were searched for the protein marker using enzyme-linked immunosorbent assay (ELISA). Seed dishes were placed in each plot, and dandelion seed removal rates were measured. The entire experiment was repeated five times over the dandelion flowering period. Gut analysis revealed that approximately 22% of specimens tested positive for the seed marker. A more diverse granivore community had trophic linkages to seeds than has been previously realized under field conditions. This community included taxa such as isopods, millipedes, weevils, rove beetles, and caterpillars, in addition to the traditionally recognized ants, crickets, and carabid beetles. Rarefaction and Chao analysis estimated approximately 16 and 27 species in the granivore communities of the Czech Republic and South Dakota, respectively. Synthesis: Generalist granivore communities are diverse and polyphagous, and are clearly important as a form of biotic resistance to invasive and weedy plants. These granivore communities can be managed to limit population growth of these pests. C1 [Lundgren, Jonathan G.] USDA ARS, NCARL, Brookings, SD 57006 USA. [Saska, Pavel; Honek, Alois] Crop Res Inst, Prague, Czech Republic. RP Lundgren, JG (reprint author), USDA ARS, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM Jonathan.Lundgren@ars.usda.gov RI Saska, Pavel/K-6434-2013 OI Saska, Pavel/0000-0003-1397-2987 FU Czech Science Foundation [526/09/1436] FX The work of P. S. and A. H. was funded by the Czech Science Foundation (# 526/09/1436). NR 47 TC 11 Z9 12 U1 2 U2 61 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD JUN PY 2013 VL 3 IS 6 BP 1642 EP 1652 DI 10.1002/ece3.580 PG 11 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA 162OJ UT WOS:000320274400019 PM 23789074 ER PT J AU Lindner, DL Carlsen, T Nilsson, RH Davey, M Schumacher, T Kauserud, H AF Lindner, Daniel L. Carlsen, Tor Nilsson, R. Henrik Davey, Marie Schumacher, Trond Kauserud, Havard TI Employing 454 amplicon pyrosequencing to reveal intragenomic divergence in the internal transcribed spacer rDNA region in fungi SO ECOLOGY AND EVOLUTION LA English DT Article DE Environmental sequencing; operational taxonomic units; pyrosequencing; species richness ID CONCERTED EVOLUTION; SPECIES IDENTIFICATION; DNA; DIVERSITY; NUCLEAR; DELIMITATION; POLYMORPHISM; SEQUENCES; SOFTWARE; ECOLOGY AB The rDNA internal transcribed spacer (ITS) region has been accepted as a DNA barcoding marker for fungi and is widely used in phylogenetic studies; however, intragenomic ITS variability has been observed in a broad range of taxa, including prokaryotes, plants, animals, and fungi, and this variability has the potential to inflate species richness estimates in molecular investigations of environmental samples. In this study 454 amplicon pyrosequencing of the ITS1 region was applied to 99 phylogenetically diverse axenic single-spore cultures of fungi (Dikarya: Ascomycota and Basidiomycota) to investigate levels of intragenomic variation. Three species (one Basidiomycota and two Ascomycota), in addition to a positive control species known to contain ITS paralogs, displayed levels of molecular variation indicative of intragenomic variation; taxon inflation due to presumed intragenomic variation was approximate to 9%. Intragenomic variability in the ITS region appears to be widespread but relatively rare in fungi (approximate to 3-5% of species investigated in this study), suggesting this problem may have minor impacts on species richness estimates relative to PCR and/or pyrosequencing errors. Our results indicate that 454 amplicon pyrosequencing represents a powerful tool for investigating levels of ITS intragenomic variability across taxa, which may be valuable for better understanding the fundamental mechanisms underlying concerted evolution of repetitive DNA regions. C1 [Lindner, Daniel L.] US Forest Serv, No Res Stn, Ctr Forest Mycol Res, Madison, WI USA. [Carlsen, Tor; Davey, Marie; Schumacher, Trond; Kauserud, Havard] Univ Oslo, Dept Biol, Microbial Evolut Res Grp MERG, NO-0316 Oslo, Norway. [Nilsson, R. Henrik] Univ Gothenburg, Dept Biol & Environm Sci, S-40530 Gothenburg, Sweden. [Davey, Marie] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, NO-1432 As, Norway. RP Lindner, DL (reprint author), Ctr Forest Mycol Res, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM dlindner@wisc.edu OI Nilsson, Henrik/0000-0002-8052-0107 FU US Forest Service; FORMAS [215-2011-498]; University of Oslo; Carl Stenholm Foundation; US Forest Service, Northern Research Station FX Funding provided by the US Forest Service, the University of Oslo, FORMAS (215-2011-498), and from the Carl Stenholm Foundation.; We would like to acknowledge Cecilie Mathiesen for lab assistance, the Norwegian Veterinary Institute and Norwegian Forest and Landscape Institute for providing fungal cultures, and Ari Jumpponen for discussions. We would also like to acknowledge the Norwegian Sequencing Center for conducting the 454 sequencing. The University of Oslo and the US Forest Service, Northern Research Station, are acknowledged for financial support. RHN acknowledges financial support from FORMAS (215-2011-498) and from the Carl Stenholm Foundation. NR 46 TC 39 Z9 39 U1 1 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD JUN PY 2013 VL 3 IS 6 BP 1751 EP 1764 DI 10.1002/ece3.586 PG 14 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA 162OJ UT WOS:000320274400028 PM 23789083 ER PT J AU Campbell, TA Long, DB Shriner, SA AF Campbell, Tyler A. Long, David B. Shriner, Susan A. TI Wildlife Contact Rates at Artificial Feeding Sites in Texas SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Bait; Collared peccary; Contact rate; Feral swine; Passive integrated transponder tag; Raccoon ID WHITE-TAILED DEER; FOOD AB Given the popularity of feeding white-tailed deer (Odocoileus virginianus) in Texas and the increasing amount of corn that is distributed, more information is needed on the impacts of this activity on non-target wildlife. Our objectives were to report visitation, intra- and interspecific contact, and contact rates of wildlife at artificial feeding sites in Texas. Our study was conducted at three sites in Kleberg and Nueces counties, Texas. We trapped animals from February to April and August to September, 2009 and marked animals with passive integrated transponder (PIT) tags. At each site and season, we placed one feeder system containing a PIT tag reader within 600 m of trap locations. Readers detected PIT tags from a distance of 25 cm. We determined a contact event to occur when two different PIT tags were detected by feeder systems within 5 s. We recorded 62,719 passes by raccoons (Procyon lotor), 103,512 passes by collared peccaries (Pecari tajacu), 2,923 passes by feral swine (Sus scrofa), 1,336 passes by fox squirrels (Sciurus niger), and no passes by opossums (Didelphis virginiana) at feeder systems. For site-season combinations in which contact events occurred, we found intraspecific contact rates (contacts per day) for raccoons, collared peccaries, and feral swine to be 0.81-124.77, 0.69-38.08, and 0.0-0.66, respectively. Throughout our study we distributed similar to 2,625 kg of whole kernel corn, which resulted in 6,351 contact events between marked wildlife (2.4 contacts per kg of corn). If 136 million kg of corn is distributed in Texas annually, we would expect > 5.2 billion unnatural contact events between wildlife would result from this activity each year in Texas. Consequently, we do not believe that it is wise for natural resource managers to maintain artificial feeding sites for white-tailed deer or other wildlife due to pathogen transmission risks. C1 [Campbell, Tyler A.; Long, David B.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr,Florida Field Stn, Gainesville, FL 32641 USA. [Shriner, Susan A.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Campbell, TA (reprint author), East Wildlife Fdn, 200 Concord Plaza,Suite 410, San Antonio, TX 78216 USA. EM tcampbell@eastfoundation.net OI Shriner, Susan/0000-0003-0349-7182 FU USDA APHIS Wildlife Services National Wildlife Research Center FX We thank Michael Benton and Texas A&M University-Kingsville for providing access to conduct research. We are grateful to Crysta Brock, Shyla Rabe, Justin Rattan, and Richie Sinclair for assistance in data collection. Financial support was provided by USDA APHIS Wildlife Services National Wildlife Research Center. Our mention of commercial products herein is for identification purposes and does not constitute endorsement or censure by the United States Department of Agriculture. All experimental procedures were approved by the Institutional Animal Care and Use Committee at the National Wildlife Research Center (Permit No. QA-1593). NR 18 TC 4 Z9 4 U1 2 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD JUN PY 2013 VL 51 IS 6 BP 1187 EP 1193 DI 10.1007/s00267-013-0046-4 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 157FS UT WOS:000319882000008 PM 23609307 ER PT J AU McManamay, RA Orth, DJ Dolloff, CA Mathews, DC AF McManamay, Ryan A. Orth, Donald J. Dolloff, Charles A. Mathews, David C. TI Application of the ELOHA Framework to Regulated Rivers in the Upper Tennessee River Basin: A Case Study SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Environmental flow; Water policy; Dams; Habitat restoration; Fish; Riparian ID ALTERED FLOW REGIMES; FISH COMMUNITIES; UNITED-STATES; STREAM FISHES; HABITAT REHABILITATION; HYDROLOGIC ALTERATION; ENVIRONMENTAL FLOWS; ADAPTIVE MANAGEMENT; SCALE-DEPENDENCE; THERMAL HABITAT AB In order for habitat restoration in regulated rivers to be effective at large scales, broadly applicable frameworks are needed that provide measurable objectives and contexts for management. The Ecological Limits of Hydrologic Alteration (ELOHA) framework was created as a template to assess hydrologic alterations, develop relationships between altered streamflow and ecology, and establish environmental flow standards. We tested the utility of ELOHA in informing flow restoration applications for fish and riparian communities in regulated rivers in the Upper Tennessee River Basin (UTRB). We followed the steps of ELOHA to generate univariate relationships between altered flows and ecology within the UTRB. By comparison, we constructed multivariate models to determine improvements in predictive capacity with the addition of non-flow variables. We then determined whether those relationships could predict fish and riparian responses to flow restoration in the Cheoah River, a regulated system within the UTRB. Although ELOHA provided a robust template to construct hydrologic information and predict hydrology for ungaged locations, our results do not suggest that univariate relationships between flow and ecology (step 4, ELOHA process) can produce results sufficient to guide flow restoration in regulated rivers. After constructing multivariate models, we successfully developed predictive relationships between flow alterations and fish/riparian responses. In accordance with model predictions, riparian encroachment displayed consistent decreases with increases in flow magnitude in the Cheoah River; however, fish richness did not increase as predicted 4 years after restoration. Our results suggest that altered temperature and substrate and the current disturbance regime may have reduced opportunities for fish species colonization. Our case study highlights the need for interdisciplinary science in defining environmental flows for regulated rivers and the need for adaptive management approaches once flows are restored. C1 [McManamay, Ryan A.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Orth, Donald J.] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Dolloff, Charles A.] US Forest Serv, USDA, Dept Fish & Wildlife Conservat, Virginia Tech, Blacksburg, VA 24061 USA. [Mathews, David C.] Tennessee Valley Author, Knoxville, TN 37902 USA. RP McManamay, RA (reprint author), Oak Ridge Natl Lab, Div Environm Sci, POB 2008, Oak Ridge, TN 37831 USA. EM mcmanamayra@ornl.gov OI Orth, Donald/0000-0002-9236-0147 FU Cheoah Fund Board; USDA Forest Service; US Fish and Wildlife Service; North Carolina Wildlife Resources Commission; NC Division of Water Resources-DENR; Sigma Xi FX This work was funded by the Cheoah Fund Board, a multi-agency collaboration among Alcoa Power, the USDA Forest Service, the US Fish and Wildlife Service, the North Carolina Wildlife Resources Commission, the NC Division of Water Resources-DENR, and other grants provided by the USDA Forest Service. Funding was also provided by the Sigma Xi Grants-In-Aid of Research Program. We thank Mark Cantrell, Chris Goodreau, Steve Fraley, Steve Reid, Jim Mead, Rick Simmons, Paul Leonard, and Andrew Bearden for providing reports, summaries, and data. Suggested revisions on earlier versions of this paper were provided by Mark Cantrell, Paul Angermeier, Emmanuel Frimpong, and Tess Wynn. In addition, comments provided by five anonymous reviewers substantially improved this paper. We also extend gratitude to Tyler Young, Toby Coyner, David Belkoski, Travis Patton, Jason Emmel, McKeever Henley, and Adam Hart for their assistance with field work. NR 105 TC 15 Z9 15 U1 6 U2 46 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD JUN PY 2013 VL 51 IS 6 BP 1210 EP 1235 DI 10.1007/s00267-013-0055-3 PG 26 WC Environmental Sciences SC Environmental Sciences & Ecology GA 157FS UT WOS:000319882000010 PM 23624994 ER PT J AU Ngo, HL Dunn, RO Hoh, E AF Ngo, Helen L. Dunn, Robert O. Hoh, Eunha TI C18-unsaturated branched-chain fatty acid isomers: Characterization and physical properties SO EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY LA English DT Article DE Branched-chain fatty acids; Iso-oleic acid; Isostearic acid; Lubricants; Methyl iso-oleate ID CATALYZED EPOXIDATION; OLEIC-ACID AB Iso-oleic acid is a mixture of C18-unsaturated branched-chain fatty acid isomers with a methyl group on various positions of the alkyl chain, which is the product of the skeletal isomerization reaction of oleic acid and is the intermediate used to make isostearic acid (C18-saturated branched-chain fatty acid isomers). Methyl iso-oleate, a mixture of C18-unsaturated branched-chain fatty acid methyl ester isomers, is obtained via acid catalyzed esterification of iso-oleic acid with methanol. The branched-chain materials are liquid at room temperature and their oiliness property makes them an attractive candidate for the lubricant industry. In this paper, we report characterization of these branched-chain materials using comprehensive two-dimensional GC with time-of-flight mass spectrometry (GCxGC/TOF-MS) and their physical and lubricity properties using tribology measurements. C1 [Ngo, Helen L.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Dunn, Robert O.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Hoh, Eunha] San Diego State Univ, Grad Sch Publ Hlth, San Diego, CA 92182 USA. RP Ngo, HL (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM helen.ngo@ars.usda.gov NR 28 TC 5 Z9 5 U1 4 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1438-7697 J9 EUR J LIPID SCI TECH JI Eur. J. Lipid Sci. Technol. PD JUN PY 2013 VL 115 IS 6 BP 676 EP 683 DI 10.1002/ejlt.201200323 PG 94 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 160IX UT WOS:000320114200012 ER PT J AU Klurfeld, DM Foreyt, J Angelopoulos, TJ Rippe, JM AF Klurfeld, D. M. Foreyt, J. Angelopoulos, T. J. Rippe, J. M. TI Lack of evidence for high fructose corn syrup as the cause of the obesity epidemic SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Editorial Material ID GLUCOSE-SWEETENED BEVERAGES; CONSUMPTION; SUCROSE; INSULIN; ENDOCRINE; MEALS; WOMEN C1 [Klurfeld, D. M.] USDA ARS, Beltsville, MD USA. [Foreyt, J.] Baylor Coll Med, Dept Med, Behav Med Res Ctr, Houston, TX 77030 USA. [Angelopoulos, T. J.] Univ Cent Florida, Appl Physiol Lab, Dept Hlth Profess, Orlando, FL 32816 USA. [Rippe, J. M.] Univ Cent Florida, Dept Biomed Sci, Orlando, FL 32816 USA. [Rippe, J. M.] Tufts Univ, Sch Med, Dept Med Cardiol, Boston, MA 02111 USA. [Klurfeld, D. M.] Rippe Lifestyle Inst, Shrewsbury, MA 01545 USA. RP Rippe, JM (reprint author), Rippe Lifestyle Inst, 21 North Quinsigamond Ave, Shrewsbury, MA 01545 USA. EM bgrady@rippelifestyle.com NR 14 TC 10 Z9 10 U1 0 U2 38 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 J9 INT J OBESITY JI Int. J. Obes. PD JUN PY 2013 VL 37 IS 6 BP 771 EP 773 DI 10.1038/ijo.2012.157 PG 3 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 161BX UT WOS:000320167200003 PM 22986683 ER PT J AU Forbes, AA Rice, LA Stewart, NB Yee, WL Neiman, M AF Forbes, A. A. Rice, L. A. Stewart, N. B. Yee, W. L. Neiman, M. TI Niche differentiation and colonization of a novel environment by an asexual parasitic wasp SO JOURNAL OF EVOLUTIONARY BIOLOGY LA English DT Article DE Diachasma alloeum; frozen niche variation; host shifting; parasitoid; phenotypic evolution ID GENERAL-PURPOSE GENOTYPES; RHAGOLETIS-POMONELLA; HOST-PLANT; CLONAL DIVERSITY; APPLE MAGGOT; POTAMOPYRGUS-ANTIPODARUM; SEXUAL REPRODUCTION; DIPTERA TEPHRITIDAE; GENETIC-VARIATION; EVOLUTION AB How do asexual taxa become adapted to a diversity of environments, and how do they persist despite changing environmental conditions? These questions are linked by their mutual focus on the relationship between genetic variation, which is often limited in asexuals, and the ability to respond to environmental variation. Asexual taxa originating from a single ancestor present a unique opportunity to assess rates of phenotypic and genetic change when access to new genetic variation is limited to mutation. Diachasma muliebre is an asexual Hymenopteran wasp that is geographically and genetically isolated from all sexual relatives. D. muliebre attack larvae of the western cherry fruit fly (Rhagoletis indifferens), which in turn feed inside bitter cherry fruit (Prunus emarginata) in August and September. R. indifferens has recently colonized a new host plant with an earlier fruiting phenology (June/July), domesticated sweet cherries (P. avium), and D. muliebre has followed its host into this temporally earlier niche. We tested three hypotheses: 1) that all D. muliebre lineages originate from a single asexual ancestor; 2) that different D. muliebre lineages (as defined by unique mtDNA haplotypes) have differentiated on their ancestral host in an important life-history trait, eclosion timing; and 3) that early-eclosing lineages have preferentially colonized the new sweet cherry niche. We find that mitochondrial COI and microsatellite data provide strong support for a single ancestral origin for all lineages. Furthermore, COI sequencing revealed five mitochondrial haplotypes among D. muliebre, and individual wasps possessing one distinctive mitochondrial haplotype (haplotype II) eclosed as reproductive adults significantly earlier than wasps with all other haplotypes. In addition, this early-eclosing lineage of D. muliebre is one of two lineages that have colonized the P. avium habitat, consistent with the preferential colonization hypothesis. These data suggest that D. muliebre has evolved adaptive phenotypic variation despite limited genetic variation, and that this variation has subsequently allowed an expansion of some wasps into a novel habitat. The D. muliebre system may allow for in-depth study of adaptation and long-term persistence of asexual taxa. C1 [Forbes, A. A.; Rice, L. A.; Stewart, N. B.; Neiman, M.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Yee, W. L.] USDA ARS, Yakima Agr Res Lab, Wapato, WA USA. RP Forbes, AA (reprint author), Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. EM andrew-forbes@uiowa.edu NR 55 TC 2 Z9 2 U1 3 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1010-061X EI 1420-9101 J9 J EVOLUTION BIOL JI J. Evol. Biol. PD JUN PY 2013 VL 26 IS 6 BP 1330 EP 1340 DI 10.1111/jeb.12135 PG 11 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 161CV UT WOS:000320170100014 PM 23517017 ER PT J AU Earles, JM Halog, A Ince, P Skog, K AF Earles, J. Mason Halog, Anthony Ince, Peter Skog, Kenneth TI Integrated Economic Equilibrium and Life Cycle Assessment Modeling for Policy-based Consequential LCA SO JOURNAL OF INDUSTRIAL ECOLOGY LA English DT Article DE biofuel; consequential life cycle assessment (CLCA); energy demand; forest economics; industrial ecology; partial equilibrium modeling ID GREENHOUSE-GAS EMISSIONS; LAND-USE CHANGE; BIOFUELS; MARKET; CONSUMPTION AB Consequential life cycle assessment (CLCA) has emerged as a tool for estimating environmental impacts of changes in product systems that go beyond physical relationships accounted for in attributional LCA (ALCA). This study builds on recent efforts to use more complex economic models for policy-based CLCA. A partial market equilibrium (PME) model, called the U.S. Forest Products Module (USFPM), is combined with LCA to analyze an energy demand scenario in which wood use increases 400 million cubic meters in the United States for ethanol production. Several types of indirect economic and environmental impacts are identified and estimated using USFPM-LCA. A key finding is that if wood use for biofuels increases to high levels and mill residue is used for biofuels and replaced by natural gas for heat and power in forest products mills, then the increased greenhouse gas emissions from natural gas could offset reductions obtained by substituting biofuels for gasoline. Such high levels of biofuel demand, however, appear to have relatively low environmental impacts across related forest product sectors. C1 [Earles, J. Mason] Univ Maine, Forest Bioprod Res Inst, Orono, ME USA. [Halog, Anthony] Univ Maine, Orono, ME USA. [Ince, Peter; Skog, Kenneth] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. RP Earles, JM (reprint author), Univ Calif Davis, Ecol Grad Grp, 2028 Acad Surge 1 Shields Ave, Davis, CA 95616 USA. EM jmearles@ucdavis.edu NR 29 TC 9 Z9 9 U1 1 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1088-1980 J9 J IND ECOL JI J. Ind. Ecol. PD JUN PY 2013 VL 17 IS 3 BP 375 EP 384 DI 10.1111/j.1530-9290.2012.00540.x PG 10 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA 159JA UT WOS:000320040500007 ER PT J AU Post, RC Eder, J Maniscako, S Johnson-Bailey, D Bard, S AF Post, Robert C. Eder, Jennifer Maniscalco, Shelley Johnson-Bailey, Donna Bard, Sasha TI MyPlate Is Now Reaching More Consumers through Social Media SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Post, Robert C.; Eder, Jennifer; Maniscalco, Shelley; Johnson-Bailey, Donna] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. [Bard, Sasha] Panum Grp, Bethesda, MD USA. RP Post, RC (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 0 TC 1 Z9 1 U1 0 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUN PY 2013 VL 113 IS 6 BP 754 EP 755 DI 10.1016/j.jand.2013.04.014 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 161US UT WOS:000320219400003 PM 23684292 ER PT J AU Friesen, KM Johnson, GD AF Friesen, Kristina M. Johnson, Gregory D. TI EVALUATION OF METHODS FOR COLLECTING BLOOD-ENGORGED MOSQUITOES FROM HABITATS WITHIN A WILDLIFE REFUGE SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Resting fiber pots; aspirator; light trap; American white pelican; West Nile virus; blood meal identification ID WEST-NILE-VIRUS; HOST-FEEDING PATTERNS; CULEX-TARSALIS DIPTERA; AMERICAN WHITE PELICAN; AEDES-VEXANS; CALIFORNIA; CULICIDAE; COUNTY; IDENTIFICATION; TRANSMISSION AB Mortality of American white pelican (Pelecanus erythrorhynchos) chicks attributed to West Nile virus (WNV) prompted field studies on the bionomics of mosquitoes on a wildlife refuge in northern Montana. One component of these studies was to identify blood meal sources for Culex tarsalis, the primary vector of WNV in the region, and the potential bridge vectors Aedes vexans and Culiseta inornata. To accomplish this, 3 methods were evaluated to collect bloodfed mosquitoes: a gasoline powered aspirator, CO2-baited light traps, and fiber pots in shelterbelts consisting of stands of deciduous trees and shrubs and marshes along the lake edge. Fiber pots were also deployed in open fields of prairie grasses. Overall, fiber pots were the most efficient method for collecting engorged Cx. tarsalis and Cs. inornata, largely due to shorter sampling and processing times. Aedes vexans was not collected in fiber pots but was more abundant in aspiration samples than the other 2 species. The optimal location for collecting Cx. tarsalis was dependent on trapping method. Aspirations and fiber pot placements collected more Cx. tarsalis in shelterbelts, while CO2-baited light traps collected more Cx. tarsalis in the marsh habitat. Sixteen avian and 4 mammalian hosts were identified from bloodfed Cx. tarsalis with 46 blood meals derived from birds and 49 from mammals. Aedes vexans and Cs. inornata fed predominantly on white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus), respectively. Humans were identified as hosts in 33% of engorged Cx. tarsalis, 4% of engorged Ae. vexans, and 18% of engorged Cs. inornata. C1 [Friesen, Kristina M.; Johnson, Gregory D.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USA. RP Friesen, KM (reprint author), USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. NR 28 TC 1 Z9 1 U1 2 U2 14 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2013 VL 29 IS 2 BP 102 EP 107 DI 10.2987/12-6323R.1 PG 6 WC Entomology SC Entomology GA 161DI UT WOS:000320171500003 PM 23923324 ER PT J AU Razon, LF Bacani, FT Evangelista, RL Knothe, G AF Razon, Luis F. Bacani, Florinda T. Evangelista, Roque L. Knothe, Gerhard TI Fatty Acid Profile of Kenaf Seed Oil SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Fatty acid profile; Gas chromatography; Hibiscus; Kenaf seed oil; Mass spectrometry; Cyclopropene fatty acids; Epoxyoleic acid ID HIBISCUS-ESCULENTUS; THESPESIA-POPULNEA; MALVACEAE; L.; IDENTIFICATION; COMPOSITES AB The fatty acid profile of kenaf (Hibiscus cannabinus L.) seed oil has been the subject of several previous reports in the literature. These reports vary considerably regarding the presence and amounts of specific fatty acids, notably (12,13-epoxy-9(Z)-octadecenoic (epoxyoleic) acid, but also cyclic (cyclopropene and cyclopropane) fatty acids. To clarify this matter, two kenaf seed oils (from the Cubano and Dowling varieties of kenaf) were investigated regarding their fatty acid profiles. Both contain epoxyoleic acid, the Cubano sample around 2 % and the Dowling sample 5-6 % depending on processing. The cyclic fatty acids malvalic and dihydrosterculic were identified in amounts around 1 %. Trace amounts of sterculic acid were observed as were minor amounts of C17:1 fatty acids. The results are discussed in the context of the fatty acid profiles of other hibiscus seed oils. C1 [Razon, Luis F.; Bacani, Florinda T.] De La Salle Univ, Dept Chem Engn, Manila 1004, Philippines. [Evangelista, Roque L.; Knothe, Gerhard] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Knothe, G (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM gerhard.knothe@ars.usda.gov NR 35 TC 9 Z9 9 U1 2 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2013 VL 90 IS 6 BP 835 EP 840 DI 10.1007/s11746-013-2230-3 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 164UC UT WOS:000320434500008 ER PT J AU Lowery, BA Andersh, B Isbell, TA AF Lowery, Benjamin A. Andersh, Brad Isbell, Terry A. TI Synthesis of Chloro Alkoxy and Alkoxy Derivatives of Methyl Oleate SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Methyl oleate; Oleic acid; Chloro alkoxy; Ether; Hypochlorous acid; Dehalogenation ID TRANSFER HYDROGENOLYSIS; HALIDES; REDUCTION; SALTS AB Vegetable oil based lubricants typically have improved lubricity and biodegradability over their mineral oil based counterparts. However, vegetable oil lubricants often fail to meet the performance standards of mineral based oils with respect to cold temperature and resistance to oxidation. Olefins are an oxidatively weak point for vegetable based compounds. Removal of the olefin in a vegetable based lubricant through functionalization may increase resistance to oxidation. If the added functionality also causes branching of the alkyl chain, cold temperature properties may be improved. Any chemical modifications considered must be scalable and cost-effective to be useful in a commercial application. In this study, methyl oleate was functionalized into a chloro alkoxy derivative. Sodium hypochlorite (household bleach) and calcium hypochlorite were both used to generate hypochlorous acid in situ. Hypochlorous acid and a series of alcohols (methanol, ethanol, and butanol) were reacted with methyl oleate to make chloro alkoxy compounds in 29.8-77.9 % yields. In an effort to make a branched saturated ether we removed the chlorine moiety of the chloro alkoxy compounds. Dehalogenation was achieved under basic conditions over a Pd/C catalyst in 2-propanol. Reaction times increased substantially as the size of the adjacent alkoxy group increased. The reaction rate could be improved by heating the reactions above 100 degrees C in a pressure reactor. Increased reaction temperature also resulted in an increase in ketone side products from the competing elimination reactions. Saturated ether yields were 4.1-43.2 %. C1 [Lowery, Benjamin A.; Isbell, Terry A.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Andersh, Brad] Bradley Univ, Dept Chem & Biochem, Peoria, IL 61625 USA. RP Isbell, TA (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM terry.isbell@ars.usda.gov NR 12 TC 0 Z9 0 U1 1 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2013 VL 90 IS 6 BP 911 EP 917 DI 10.1007/s11746-013-2224-1 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 164UC UT WOS:000320434500016 ER PT J AU Overturf, K Welker, T Barrows, F Towner, R Schneider, R LaPatra, S AF Overturf, Ken Welker, Tom Barrows, Frederick Towner, Richard Schneider, Rich LaPatra, Scott TI Variation in Rainbow Trout, Oncorhynchus mykiss, to Biosynthesize Eicosapentaenoic Acid and Docosahexaenoic Acid When Reared on Plant Oil Replacement Feeds SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID SALMON SALMO-SALAR; BREAM SPARUS-AURATA; DIETARY FISH-OIL; FATTY-ACIDS; MARINE FISH; VEGETABLE-OILS; LINOLENIC ACID; RAPESEED OIL; GROWTH; METABOLISM AB To determine the potential for improving the conversion and deposition of the omega-3 fatty acids docosahexaenoic acid (DHA; 22:6n-3) and eicosapentaenoic acid (EPA; 20:5n-3) in fish, 44 families of rainbow trout were fed a diet low in these components and then evaluated for their ability to convert and store plant oils that did not contain DHA and EPA in their muscle tissue. The range for EPA and DHA detected in the tissues of different families varied between 1.2 and 2.9%, and 3.8 and 7.1% total fatty acid, respectively. After adjusting for covariates, an averaged heritability of 0.78 +/- 0.11 for DHA and 0.61 +/- 0.17 for EPA was calculated. Expression analysis of genes related to the elongation and conversion of lipids were also analyzed, and significant differences were found in the expression of some genes between groups of families that were delineated as having relative high, medium, and low capabilities of depositing EPA and DHA in their muscle tissue after being reared on the mainly soy/flax oil containing diet. However, none of these genes showed a positive correlation with the high conversion/deposition group. C1 [Overturf, Ken; Welker, Tom; Barrows, Frederick] ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Towner, Richard] GenTec Consulting, Payette, ID 83661 USA. [Schneider, Rich; LaPatra, Scott] Clear Springs Foods Inc, Div Res, Buhl, ID 83316 USA. RP Overturf, K (reprint author), ARS, USDA, Hagerman Fish Culture Expt Stn, 3059-F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. NR 52 TC 2 Z9 2 U1 3 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD JUN PY 2013 VL 44 IS 3 BP 326 EP 337 DI 10.1111/jwas.12041 PG 12 WC Fisheries SC Fisheries GA 161DK UT WOS:000320171700002 ER PT J AU Mun, SP Cai, ZY Zhang, JL AF Mun, Sung Phil Cai, Zhiyong Zhang, Jilei TI Fe-catalyzed thermal conversion of sodium lignosulfonate to graphene SO MATERIALS LETTERS LA English DT Article DE Lignosulfonate; Sulfite pulping; Fe nanoparticles; Graphene; Thermal treatment; Raman spectrum ID WOOD CHARCOAL; CARBON; NANOPARTICLES; SHEETS; LIGNIN AB Sodium lignosulfonate (LS) from sulfite pulping processing was used as a carbon source to synthesize graphene. LS was mixed with Fe nanoparticles (FeNPs) as a catalyst and thermally treated at 1000 C for 1 h. The Raman spectrum and X-ray diffraction pattern suggested that graphene sheets were formed in LS thermally treated with FeNPs (Fe-HTLS). Scanning electron microscopy image of Fe-HTLS showed clusters of thin graphene sheets appearing in the form of nanoflowers. High-resolution transmission electron microscopy and electron reflection images provided further evidence of the formation of graphene in LS thermally treated in the presence of Fe catalyst. (C) 2013 Elsevier B.V. All rights reserved. C1 [Mun, Sung Phil] Chonbuk Natl Univ, Dept Wood Sci & Technol, Jeonju 561756, Jeonbuk, South Korea. [Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Zhang, Jilei] Mississippi State Univ, Dept Forest Prod, Mississippi State, MS 39762 USA. RP Zhang, JL (reprint author), Mississippi State Univ, Dept Forest Prod, Mississippi State, MS 39762 USA. EM jzhang@cfr.msstate.edu FU USDA Forest Service [11JV1111112409711121332] FX This work was supported by the USDA Forest Service through Grant no. 11JV1111112409711121332. The authors would like to acknowledge Dr. Fumiya Watanabe (Nanotechnology Center, the University of Arkansas at Little Rock) in taking the high resolution TEM images and Dr. Shane C. Street (University of Alabama) for allowing us to use the FT-Raman spectrophotometer. This manuscript was approved for publication as Journal Article no FP 667 of the Forest and Wildlife Research Center, Mississippi State University. NR 16 TC 6 Z9 6 U1 8 U2 61 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-577X J9 MATER LETT JI Mater. Lett. PD JUN 1 PY 2013 VL 100 BP 180 EP 183 DI 10.1016/j.matlet.2013.02.101 PG 4 WC Materials Science, Multidisciplinary; Physics, Applied SC Materials Science; Physics GA 159XA UT WOS:000320079400049 ER PT J AU Willcox, MC Davis, GL Warburton, ML Windham, GL Abbas, HK Betran, J Holland, JB Williams, WP AF Willcox, Martha C. Davis, Georgia L. Warburton, Marilyn L. Windham, Gary L. Abbas, Hamed K. Betran, Javier Holland, James B. Williams, W. Paul TI Confirming quantitative trait loci for aflatoxin resistance from Mp313E in different genetic backgrounds SO MOLECULAR BREEDING LA English DT Article DE Aspergillus flavus; Aflatoxin; Quantitative trait loci; Maize (Zea mays L.); Host plant resistance; Molecular markers; Genetic background ID ASPERGILLUS EAR ROT; KERNEL INFECTION; GERMPLASM LINE; CONTRIBUTING RESISTANCE; MAIZE; ACCUMULATION; REGISTRATION; FLAVUS; CORN; IDENTIFICATION AB The fungus Aspergillus flavus (Link:Fr) causes ear rot of maize (Zea mays L.) and produces the toxic metabolic product aflatoxin. One particularly effective method of controlling the fungus is via host plant resistance, but while several resistant breeding lines have been identified, transferring the resistance genes from these lines into elite cultivars has been less effective than needed. A high number of genes involved with resistance, each with a small effect, and some only found under certain environmental conditions, has hampered resistance breeding. The identification of markers linked to genomic regions associated with resistance would aid in this effort. The goals of this study were to identify and characterize quantitative trait loci (QTL) conferring resistance to aflatoxin accumulation from resistant maize donor Mp313E in a background of the susceptible inbred line Va35; to compare them to the QTL identified from Mp313E in a background of B73; and to test the stability of the QTL identified in Mp313E x Va35 in multiple environments by remapping the phenotypic tails of the Mp313E x Va35 mapping population in new locations. Twenty different QTL were found in this study, 11 of which were also found in different environments using the phenotypic tail subset mapping population, and five of which were likely the same as those reported in the Mp313E x B73 mapping population. This indicates that many of the QTL are stable over the environments and genetic backgrounds tested, which will make them more valuable in breeding efforts. C1 [Willcox, Martha C.] CIMMYT Int, Mexico City 06600, DF, Mexico. [Davis, Georgia L.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Warburton, Marilyn L.; Windham, Gary L.; Williams, W. Paul] ARS, USDA, CHPRRU, Mississippi State, MS 39762 USA. [Abbas, Hamed K.] ARS, USDA, BCPRU, Stoneville, MS 38776 USA. [Betran, Javier] Syngenta Seeds SAS, F-31151 Fenouillet, France. [Holland, James B.] ARS, USDA, PSRU, Raleigh, NC 27695 USA. RP Warburton, ML (reprint author), ARS, USDA, CHPRRU, POB 9555, Mississippi State, MS 39762 USA. EM mwarburton@cgiar.org OI Holland, James/0000-0002-4341-9675 NR 33 TC 15 Z9 16 U1 1 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD JUN PY 2013 VL 32 IS 1 BP 15 EP 26 DI 10.1007/s11032-012-9821-9 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 165DK UT WOS:000320463000002 ER PT J AU Imai, I Kimball, JA Conway, B Yeater, KM McCouch, SR McClung, A AF Imai, Ize Kimball, Jennifer A. Conway, Benjamin Yeater, Kathleen M. McCouch, Susan R. McClung, Anna TI Validation of yield-enhancing quantitative trait loci from a low-yielding wild ancestor of rice SO MOLECULAR BREEDING LA English DT Article DE Near isogenic lines; Introgression lines; Crop yield; Quantitative trait loci; Genetic analysis; Genotypes ID MARKER-ASSISTED SELECTION; ORYZA-SATIVA; DROUGHT-RESISTANCE; GRAIN-YIELD; UPLAND RICE; GENETIC ARCHITECTURE; LOWLAND DROUGHT; BREAD WHEAT; QTL; GENOME AB A set of introgression lines (ILs) containing chromosomal segments from O. rufipogon (IRGC 105491), a wild relative of O. sativa, in the genetic background of an elite US variety, cv. Jefferson, was developed to confirm the performance of six yield-enhancing quantitative trait loci (QTL). Fifty BC3F3 ILs containing homozygous O. rufipogon introgressions at each of the target QTL regions, and as few background introgressions as possible, were selected for evaluation of yield and 14 yield-related traits in field studies conducted over 2 years at four locations in the southern USA. Performance of the IL families was compared with three commercial inbreds and one hybrid variety. IL families carrying introgressions from the low-yielding wild parent at the QTL yld2.1 and yld6.1 yielded 27.7 and 26.1 % more than Jefferson, respectively. IL yld2A, which possesses yld2.1, also performed well under alternate wetting and drying conditions in two field locations. After the first year of field trials, 10 of the top-performing BC3F4 families, representing five of the QTL targets, were genotyped with an Illumina 1,536 assay to define the size and location of the wild introgressions. BC3F4 families with the fewest background introgressions were backcrossed to Jefferson and selfed. The resulting BC4F2 families were screened with targeted single nucleotide polymorphism assays to identify individuals carrying homozygous introgressions across the target QTL. Twelve ILs, representing each of the six QTL targets, have been submitted to the Genetic Stocks Oryza Collection for studies on transgressive variation and as interspecific pre-breeding lines. C1 [Imai, Ize; McCouch, Susan R.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Kimball, Jennifer A.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Conway, Benjamin] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Yeater, Kathleen M.] ARS, USDA, SPA, College Stn, TX 77840 USA. [McClung, Anna] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP McCouch, SR (reprint author), Cornell Univ, Dept Plant Breeding & Genet, 162 Emerson Hall, Ithaca, NY 14853 USA. EM srm4@cornell.edu FU NSF-TV Plant Genome Research Program Award [0606461, 1026555]; USDA-NIFA Award [2009-65300-05698] FX We thank Paul Doran, Hema Liyanage and Bob Shiels from Sequenom, Inc., for technical assistance; Shannon Moon and RiceTec, Inc., for collection of field data in Jonesboro and Alvin; Tony Beaty, Laduska Simpson, Sarah Hendrix, Bill Luebke and Curtis Kerns for yield trials at Stuttgart; Jodie Cammack, Kip Landry, Carl Henry, Piper Roberts and Jason Bonnette for yield trials at Beaumont; WenGui Yan and Tiffany Sookaserm for sheath blight and straighthead evaluations; Gen Onishi and Sandy Harrington for help with crossing and managing populations at Cornell University; collaborators in the Uniform Regional Rice Nursery for agronomic, grain quality, genetic markers, and biotic and abiotic stress data; and Cheryl Utter for formatting the manuscript. This study is funded by NSF-TV Plant Genome Research Program Award #0606461 and #1026555 and USDA-NIFA Award #2009-65300-05698. NR 48 TC 16 Z9 16 U1 1 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD JUN PY 2013 VL 32 IS 1 BP 101 EP 120 DI 10.1007/s11032-013-9855-7 PG 20 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 165DK UT WOS:000320463000008 ER PT J AU McNeel, AK Cushman, RA Vallet, JL AF McNeel, Anthony K. Cushman, Robert A. Vallet, Jeffrey L. TI The plasminogen activator system in the ovine placentome during late gestation and stage-two of parturition SO MOLECULAR REPRODUCTION AND DEVELOPMENT LA English DT Article ID POSTPARTUM UTERINE DISEASES; GENE-EXPRESSION; MATRIX METALLOPROTEINASES; FETAL MEMBRANES; TISSUE-TYPE; DAIRY-COWS; RECEPTOR; INHIBITOR-1; RELEASE; MECHANISM AB The process of placental separation is not completely understood. In domestic animals, especially cattle, it is important that expulsion of the fetal membranes takes place in a timely manner in order to achieve maximal reproductive efficiency. The activity of the matrix-metalloprotease (MMP) family of proteases is known to be reduced in placentomes from cases of retained placenta. Members of the MMP family are known to be activated by the plasminogen activator (PA) family of proteases. We hypothesized that the expression and activity of the PA family increase in the cotyledon and/or caruncle as parturition approaches, with maximal expression and activity at parturition. To test this hypothesis, we performed reverse-transcriptase quantitative PCR and plasminogen-casein zymography to detect the presence and activity of PA family members in the placentome leading up to and during parturition in spontaneous and dexamethasone-induced parturient ewes. The results from our experiments indicated that serine proteases inhibitor E1 (SERPINE1) mRNA abundance in the cotyledon was different between treatment groups (P=0.0002). In the caruncle, gene expression for plasminogen activator urokinase-type (PLAU) was different (P=0.0154), and there was a strong trend for differences in SERPINE1 expression (P=0.0565). These results demonstrate that expression of the PA system in the placentome changes from late pregnancy to parturition, and the presence or activity of these enzymes may occur after fetal expulsion. Mol. Reprod. Dev. 80: 466-473, 2013. (c) 2013 Wiley Periodicals, Inc. C1 [McNeel, Anthony K.; Cushman, Robert A.; Vallet, Jeffrey L.] ARS, USDA, US Meat Anim Res Ctr, Reprod Res Unit, Clay Ctr, NE 68933 USA. RP McNeel, AK (reprint author), ARS, USDA, US Meat Anim Res Ctr, Reprod Res Unit, POB 166, Clay Ctr, NE 68933 USA. EM anthony.mcneel@ars.usda.gov NR 36 TC 1 Z9 1 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1040-452X J9 MOL REPROD DEV JI Mol. Reprod. Dev. PD JUN PY 2013 VL 80 IS 6 BP 466 EP 473 DI 10.1002/mrd.22183 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Reproductive Biology SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Reproductive Biology GA 167VL UT WOS:000320663200006 PM 23585221 ER PT J AU Majumdar, R Shao, L Minocha, R Long, S Minocha, SC AF Majumdar, Rajtilak Shao, Lin Minocha, Rakesh Long, Stephanie Minocha, Subhash C. TI Ornithine: The Overlooked Molecule in the Regulation of Polyamine Metabolism(3) SO PLANT AND CELL PHYSIOLOGY LA English DT Article DE Arabidopsis thaliana; Polyamines; Metabolism; Ornithine; Putrescine ID POPLAR CELLS; DECARBOXYLASE CDNA; SALT STRESS; PUTRESCINE BIOSYNTHESIS; ARGININE DECARBOXYLASE; GENETIC MANIPULATION; CELLULAR POLYAMINES; FUNCTIONAL-ANALYSIS; MAMMALIAN-CELLS; OVER-EXPRESSION AB We overexpressed a mouse ornithine decarboxylase gene under the control of a constitutive and an estradiol-inducible promoter in Arabidopsis thaliana to increase our understanding of the regulation of polyamine metabolism. Of particular interest was the role of the substrate ornithine not only in the regulation of polyamine biosynthesis, but also in the accumulation of related amino acids in response to short-term induction of this enzyme. We hypothesized that the inducible expression of the transgene would mimic the natural responses of plants to changing conditions, e.g. under stress conditions and during rapid growth. Our results reveal that ornithine, even though present in relatively small quantities (compared with other amino acids of the glutamate-arginine-proline pathway), may not only be the key regulator of polyamine biosynthesis in Arabidopsis, but it may also regulate the entire subset of pathways for glutamate to arginine and to proline. Indirectly, it could also regulate putrescine catabolism, therefore contributing to the gamma-aminobutyric acid content of the cells. Furthermore, the induction of mouse ornithine decarboxylase resulted in up- and down-regulation of several amino acids in the transgenic plants. It was learned that the turnover of putrescine in both the wild type and the transgenic plants occurs rapidly, with a half-life of 6-8 h. C1 [Majumdar, Rajtilak; Shao, Lin; Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Minocha, Rakesh; Long, Stephanie] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Minocha, SC (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. EM sminocha@unh.edu FU United States Department of Agriculture Forest Service; New Hampshire Agricultural Experiment Station; College of Life Sciences and Agriculture; University of New Hampshire Graduate School FX The United States Department of Agriculture Forest Service; the New Hampshire Agricultural Experiment Station; the College of Life Sciences and Agriculture [financial assistance to R. Majumdar and L.S.]; the University of New Hampshire Graduate School [financial assistance to R. Majumdar and L.S.]. NR 70 TC 22 Z9 22 U1 1 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-0781 J9 PLANT CELL PHYSIOL JI Plant Cell Physiol. PD JUN PY 2013 VL 54 IS 6 BP 990 EP 1004 DI 10.1093/pcp/pct053 PG 15 WC Plant Sciences; Cell Biology SC Plant Sciences; Cell Biology GA 160JS UT WOS:000320116400015 PM 23574701 ER PT J AU Al Rahim, M Rimando, AM Silistreli, K El-Alfy, AT AF Al Rahim, Md. Rimando, Agnes M. Silistreli, Kalpten El-Alfy, Abir T. TI Anxiolytic Action of Pterostilbene: Involvement of Hippocampal ERK Phosphorylation SO PLANTA MEDICA LA English DT Article DE pterostilbene; anxiolytic activity; elevated plus maze; ERK phosphorylation; hippocampus ID SIGNAL-REGULATED KINASE; PHARMACOLOGICAL-TREATMENT; RESVERATROL ANALOGS; ANXIETY DISORDERS; VACCINIUM BERRIES; MEMORY; MICE; PICEATANNOL; COMORBIDITY; INHIBITION AB Pterostilbene, a natural analog of resveratrol, has diverse health-beneficial properties. However, the neurological activities of this compound are largely unexplored. Here, we report that pterostilbene shows anxiolytic-like actions by down-regulating phosphorylated levels of extracellular regulated kinases in the hippocampus of mice. Adult male mice administered pterostilbene (1-10 mg/kg, p.o.) were subjected to the elevated plus maze test. Pterostilbene manifested anxiolytic activity at 1 and 2 mg/kg doses, demonstrated by increases in % permanence time and number of open arm entries. The locomotor activity of the animals was unaffected at all doses. Western blot analysis revealed a decrease in both extracellular regulated kinase1 and extracellular regulated kinase 2 phosphorylation in hippocampal homogenates from mice treated with 1 and 2 mg/kg pterostilbene. Moreover, pterostilbene was detected in the plasma and brains of mice following single oral administration. Anxiolytic activity was not observed at the higher doses (5 and 10 mg/kg). However, no impairment of motor function was observed either, suggesting a favorable safety index for the compound. These results suggest that pterostilbene has the potential for therapeutic drug development for anxiety disorders. C1 [Al Rahim, Md.; Silistreli, Kalpten; El-Alfy, Abir T.] Univ Mississippi, Coll Pharm, Dept Pharmacol, University, MS 38677 USA. [Rimando, Agnes M.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS USA. RP El-Alfy, AT (reprint author), Chicago State Univ, Coll Pharm, Dept Pharmaceut Sci, 9501 S King Dr,DH 206, Chicago, IL 60628 USA. EM ael-alfy@csu.edu RI RAHIM, MD/K-5954-2013 FU National Center for Research Resources [5P20RR021929] FX We thank Gloria Hervey for her excellent technical help. We acknowledge the financial support provided by grant number 5P20RR021929 from the National Center for Research Resources for Dr. Md. Al Rahim. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Research Resources or the National Institutes of Health. NR 32 TC 5 Z9 5 U1 0 U2 4 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 J9 PLANTA MED JI Planta Med. PD JUN PY 2013 VL 79 IS 9 BP 723 EP 730 DI 10.1055/s-0032-1328553 PG 8 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA 164ZJ UT WOS:000320451500002 PM 23677525 ER PT J AU Powell, MR AF Powell, Mark R. TI How to Model a Negligible Probability Under the WTO Sanitary and Phytosanitary Agreement? SO RISK ANALYSIS LA English DT Article DE Risk assessment; sanitary and phytosanitary agreement; World Trade Organization AB Since the 1997 EC - Hormones decision, World Trade Organization (WTO) Dispute Settlement Panels have wrestled with the question of what constitutes a negligible risk under the Sanitary and Phytosanitary Agreement. More recently, the 2010 WTO Australia - Apples Panel focused considerable attention on the appropriate quantitative model for a negligible probability in a risk assessment. The 2006 Australian Import Risk Analysis for Apples from New Zealand translated narrative probability statements into quantitative ranges. The uncertainty about a negligible probability was characterized as a uniform distribution with a minimum value of zero and a maximum value of 10-6. The Australia - Apples Panel found that the use of this distribution would tend to overestimate the likelihood of negligible events and indicated that a triangular distribution with a most probable value of zero and a maximum value of 10-6 would correct the bias. The Panel observed that the midpoint of the uniform distribution is 5 x 10-7 but did not consider that the triangular distribution has an expected value of 3.3 x 10-7. Therefore, if this triangular distribution is the appropriate correction, the magnitude of the bias found by the Panel appears modest. The Panel's detailed critique of the Australian risk assessment, and the conclusions of the WTO Appellate Body about the materiality of flaws found by the Panel, may have important implications for the standard of review for risk assessments under the WTO SPS Agreement. C1 USDA, Off Risk Assessment & Cost Benefit Anal, Washington, DC 20250 USA. RP Powell, MR (reprint author), USDA, Off Risk Assessment & Cost Benefit Anal, 1400 Independence Ave,SW MS 3811, Washington, DC 20250 USA. EM mpowell@oce.usda.gov NR 34 TC 0 Z9 0 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD JUN PY 2013 VL 33 IS 6 BP 972 EP 983 DI 10.1111/j.1539-6924.2012.01895.x PG 12 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA 161IA UT WOS:000320184900003 PM 22985254 ER PT J AU Ager, AA Buonopane, M Reger, A Finney, MA AF Ager, Alan A. Buonopane, Michelle Reger, Allison Finney, Mark A. TI Wildfire Exposure Analysis on the National Forests in the Pacific Northwest, USA SO RISK ANALYSIS LA English DT Article DE Burn probability; exposure analysis; national forests; risk assessment; wildfire risk ID WILDLAND-URBAN INTERFACE; WESTERN UNITED-STATES; FUEL TREATMENTS; FIRE MANAGEMENT; RISK-ASSESSMENT; LANDSCAPE; IMPLEMENTATION; SIMULATION; FRAMEWORK; OREGON AB We analyzed wildfire exposure for key social and ecological features on the national forests in Oregon and Washington. The forests contain numerous urban interfaces, old growth forests, recreational sites, and habitat for rare and endangered species. Many of these resources are threatened by wildfire, especially in the east Cascade Mountains fire-prone forests. The study illustrates the application of wildfire simulation for risk assessment where the major threat is from large and rare naturally ignited fires, versus many previous studies that have focused on risk driven by frequent and small fires from anthropogenic ignitions. Wildfire simulation modeling was used to characterize potential wildfire behavior in terms of annual burn probability and flame length. Spatial data on selected social and ecological features were obtained from Forest Service GIS databases and elsewhere. The potential wildfire behavior was then summarized for each spatial location of each resource. The analysis suggested strong spatial variation in both burn probability and conditional flame length for many of the features examined, including biodiversity, urban interfaces, and infrastructure. We propose that the spatial patterns in modeled wildfire behavior could be used to improve existing prioritization of fuel management and wildfire preparedness activities within the Pacific Northwest region. C1 [Ager, Alan A.] US Forest Serv, USDA, Pacific NW Res Stn, Western Wildland Environm Threat Assessment Ctr, Prineville, OR 97754 USA. [Buonopane, Michelle] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. [Reger, Allison] US Forest Serv, USDA, Willamette Natl Forest, Eugene, OR 97477 USA. [Finney, Mark A.] US Forest Serv, USDA, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. RP Ager, AA (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Western Wildland Environm Threat Assessment Ctr, 3160 NE 3rd St, Prineville, OR 97754 USA. EM aager@fs.fed.us FU Forest Service Western Wildland Environmental Threat Assessment Center; Fire and Aviation, Pacific Northwest Region FX This project was funded by the Forest Service Western Wildland Environmental Threat Assessment Center and Fire and Aviation, Pacific Northwest Region. Dave Calkin and Matt Thompson from the Rocky Mountain Research Station contributed to the development of this work during work on the Cohesive Strategy Science Report. We thank Ray Davis for sharing the northern spotted owl home range data. Dana Simon of the Deschutes National Forest provided GIS support. NR 82 TC 12 Z9 12 U1 3 U2 40 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD JUN PY 2013 VL 33 IS 6 BP 1000 EP 1020 DI 10.1111/j.1539-6924.2012.01911.x PG 21 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA 161IA UT WOS:000320184900005 PM 23078351 ER PT J AU Wibbenmeyer, MJ Hand, MS Calkin, DE Venn, TJ Thompson, MP AF Wibbenmeyer, Matthew J. Hand, Michael S. Calkin, David E. Venn, Tyron J. Thompson, Matthew P. TI Risk Preferences in Strategic Wildfire Decision Making: A Choice Experiment with U.S. Wildfire Managers SO RISK ANALYSIS LA English DT Article DE Fire management; nonexpected utility theory; risa preferences ID PROBABILITY WEIGHTING FUNCTION; WILDLAND FIRE SUPPRESSION; FOREST-SERVICE; CONTINGENT VALUATION; NONRESPONSE BIAS; PROSPECT-THEORY; UNITED-STATES; UNCERTAINTY; UTILITY; MODEL AB Federal policy has embraced risa management as an appropriate paradigm for wildfire management. Economic theory suggests that over repeated wildfire events, potential economic costs and risas of ecological damage are optimally balanced when management decisions are free from biases, risa aversion, and risa seeking. Of primary concern in this article is how managers respond to wildfire risa, including the potential effect of wildfires (on ecological values, structures, and safety) and the likelihood of different fire outcomes. We use responses to a choice experiment questionnaire of U.S. federal wildfire managers to measure attitudes toward several components of wildfire risa and to test whether observed risa attitudes are consistent with the efficient allocation of wildfire suppression resources. Our results indicate that fire managers' decisions are consistent with nonexpected utility theories of decisions under risa. Managers may overallocate firefighting resources when the likelihood or potential magnitude of damage from fires is low, and sensitivity to changes in the probability of fire outcomes depends on whether probabilities are close to one or zero and the magnitude of the potential harm. C1 [Wibbenmeyer, Matthew J.] Univ Calif Santa Barbara, Dept Econ, Santa Barbara, CA 93106 USA. [Hand, Michael S.; Calkin, David E.; Thompson, Matthew P.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Venn, Tyron J.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Wibbenmeyer, Matthew J.] Collins Consulting, Missoula, MT USA. RP Hand, MS (reprint author), POB 7669, Missoula, MT 59807 USA. EM mshand@fs.fed.us RI Calkin, David/F-4727-2014 NR 55 TC 10 Z9 12 U1 2 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD JUN PY 2013 VL 33 IS 6 BP 1021 EP 1037 DI 10.1111/j.1539-6924.2012.01894.x PG 17 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA 161IA UT WOS:000320184900006 PM 23078036 ER PT J AU Newcomer, BW Neill, JD Marley, MS Ridpath, JF Givens, MD AF Newcomer, Benjamin W. Neill, John D. Marley, M. Shonda Ridpath, Julia F. Givens, M. Daniel TI Mutations induced in the NS5B gene of bovine viral diarrhea virus by antiviral treatment convey resistance to the compound SO VIRUS RESEARCH LA English DT Article DE Bovine viral diarrhea virus; Antiviral; NS5B; Cattle ID DEPENDENT RNA-POLYMERASE; PESTIVIRUS RNA; CELL LINES; REPLICATION; INFECTIONS; INHIBITION; VACCINES; GROWTH AB Bovine viral diarrhea virus (BVDV) is a widespread bovine pathogen for which there is no specific therapeutic agent. A previous study using 2-(2-benzimidazoly1)-5-[4-(2-imidazolino)phenyl]furan dihydrochloride (DB772) to treat calves persistently infected with BVDV resulted in a decrease in the viral load of infected calves but treatment resulted in the rapid selection of drug-resistant mutant isolates. In this article we describe three mutations found in the mutant isolates associated with in vivo and in vitro resistance to DB772. All three mutations are found in the NS5B which functions as the RNA-dependentRNA-polymerase during viral replication. Growth curves for the mutant isolates were not largely different from those of wild-type isolates when cultured in the absence of DB772. Thus, DB772 appears to act by binding to the specified domain but binding is disrupted or inhibited by the described mutation. (C) 2013 Elsevier B.V. All rights reserved. C1 [Newcomer, Benjamin W.] Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. [Neill, John D.; Ridpath, Julia F.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Marley, M. Shonda; Givens, M. Daniel] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. RP Newcomer, BW (reprint author), Auburn Univ, Coll Vet Med, Dept Clin Sci, 1500 Wire Rd, Auburn, AL 36849 USA. EM bwn0001@auburn.edu; john.neill@ars.usda.gov; edensms@auburn.edu; jullasidpath@ars.usda.gov; givenmd@auburn.edu NR 29 TC 3 Z9 3 U1 2 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD JUN PY 2013 VL 174 IS 1-2 BP 95 EP 100 DI 10.1016/j.virusres.2013.03.007 PG 6 WC Virology SC Virology GA 156AH UT WOS:000319791700012 PM 23524138 ER PT J AU Tooze, JA Troiano, RP Carroll, RJ Moshfegh, AJ Freedman, LS AF Tooze, Janet A. Troiano, Richard P. Carroll, Raymond J. Moshfegh, Alanna J. Freedman, Laurence S. TI A Measurement Error Model for Physical Activity Level as Measured by a Questionnaire With Application to the 19992006 NHANES Questionnaire SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE Berkson model; bias; energy metabolism; measurement error model; models; statistical; motor activity; self-assessment ID DOUBLY LABELED WATER; DIETARY MEASUREMENT ERROR; ENERGY-EXPENDITURE; PROTEIN-INTAKE; RELATIVE RISK; VALIDATION; HUMANS; LIMITATIONS; BIOMARKERS AB Systematic investigations into the structure of measurement error of physical activity questionnaires are lacking. We propose a measurement error model for a physical activity questionnaire that uses physical activity level (the ratio of total energy expenditure to basal energy expenditure) to relate questionnaire-based reports of physical activity level to true physical activity levels. The 19992006 National Health and Nutrition Examination Survey physical activity questionnaire was administered to 433 participants aged 4069 years in the Observing Protein and Energy Nutrition (OPEN) Study (Maryland, 19992000). Valid estimates of participants total energy expenditure were also available from doubly labeled water, and basal energy expenditure was estimated from an equation; the ratio of those measures estimated true physical activity level (truth). We present a measurement error model that accommodates the mixture of errors that arise from assuming a classical measurement error model for doubly labeled water and a Berkson error model for the equation used to estimate basal energy expenditure. The method was then applied to the OPEN Study. Correlations between the questionnaire-based physical activity level and truth were modest (r 0.320.41); attenuation factors (0.430.73) indicate that the use of questionnaire-based physical activity level would lead to attenuated estimates of effect size. Results suggest that sample sizes for estimating relationships between physical activity level and disease should be inflated, and that regression calibration can be used to provide measurement erroradjusted estimates of relationships between physical activity and disease. C1 [Tooze, Janet A.] Wake Forest Sch Med, Dept Biostat Sci, Div Publ Hlth Sci, Winston Salem, NC 27157 USA. [Troiano, Richard P.] NCI, Risk Factor Monitoring & Methods Branch, Appl Res Program, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [Carroll, Raymond J.] Texas A&M Univ, Dept Stat, Intercollegiate Fac Nutr, College Stn, TX 77843 USA. [Carroll, Raymond J.] Texas A&M Univ, Interdisciplinary Fac Toxicol, College Stn, TX USA. [Moshfegh, Alanna J.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Freedman, Laurence S.] Chaim Sheba Med Ctr, Gertner Inst Epidemiol & Hlth Policy Res, IL-52621 Tel Hashomer, Israel. RP Tooze, JA (reprint author), Wake Forest Sch Med, Dept Biostat Sci, Med Ctr Blvd, Winston Salem, NC 27157 USA. EM jtooze@wakehealth.edu OI Troiano, Richard/0000-0002-6807-989X FU National Cancer Institute at the National Institutes of Health [R21CA139291, R27CA057030] FX This work was supported by the National Cancer Institute at the National Institutes of Health (grant R21CA139291 for J.A.T., R.J.C., and L. S. F.; and grant R27CA057030 for R.J.C.). NR 32 TC 18 Z9 18 U1 3 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD JUN 1 PY 2013 VL 177 IS 11 BP 1199 EP 1208 DI 10.1093/aje/kws379 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 155MM UT WOS:000319752400005 PM 23595007 ER PT J AU Lackey, DE Lynch, CJ Olson, KC Mostaedi, R Ali, M Smith, WH Karpe, F Humphreys, S Bedinger, DH Dunn, TN Thomas, AP Oort, PJ Kieffer, DA Amin, R Bettaieb, A Haj, FG Permana, P Anthony, TG Adams, SH AF Lackey, Denise E. Lynch, Christopher J. Olson, Kristine C. Mostaedi, Rouzbeh Ali, Mohamed Smith, William H. Karpe, Fredrik Humphreys, Sandy Bedinger, Daniel H. Dunn, Tamara N. Thomas, Anthony P. Oort, Pieter J. Kieffer, Dorothy A. Amin, Rajesh Bettaieb, Ahmed Haj, Fawaz G. Permana, Paska Anthony, Tracy G. Adams, Sean H. TI Regulation of adipose branched-chain amino acid catabolism enzyme expression and cross-adipose amino acid flux in human obesity SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE bariatric; diabetes; hyperinsulinemia; mammalian target of rapamycin; protein ID TISSUE BLOOD-FLOW; INSULIN-RESISTANCE; YOUNG-ADULTS; IN-VIVO; METABOLISM; DEHYDROGENASE; LEUCINE; WOMEN; GENE; RAT AB Elevated blood branched-chain amino acids (BCAA) are often associated with insulin resistance and type 2 diabetes, which might result from a reduced cellular utilization and/or incomplete BCAA oxidation. White adipose tissue (WAT) has become appreciated as a potential player in whole body BCAA metabolism. We tested if expression of the mitochondrial BCAA oxidation checkpoint, branched-chain alpha-ketoacid dehydrogenase (BCKD) complex, is reduced in obese WAT and regulated by metabolic signals. WAT BCKD protein (E1 alpha subunit) was significantly reduced by 35-50% in various obesity models (fa/fa rats, db/db mice, diet-induced obese mice), and BCKD component transcripts significantly lower in subcutaneous (SC) adipocytes from obese vs. lean Pima Indians. Treatment of 3T3-L1 adipocytes or mice with peroxisome proliferator-activated receptor-gamma agonists increased WAT BCAA catabolism enzyme mRNAs, whereas the nonmetabolizable glucose analog 2-deoxy-D-glucose had the opposite effect. The results support the hypothesis that suboptimal insulin action and/or perturbed metabolic signals in WAT, as would be seen with insulin resistance/type 2 diabetes, could impair WAT BCAA utilization. However, cross-tissue flux studies comparing lean vs. insulin-sensitive or insulin-resistant obese subjects revealed an unexpected negligible uptake of BCAA from human abdominal SC WAT. This suggests that SC WAT may not be an important contributor to blood BCAA phenotypes associated with insulin resistance in the overnight-fasted state. mRNA abundances for BCAA catabolic enzymes were markedly reduced in omental (but not SC) WAT of obese persons with metabolic syndrome compared with weight-matched healthy obese subjects, raising the possibility that visceral WAT contributes to the BCAA metabolic phenotype of metabolically compromised individuals. C1 [Lackey, Denise E.; Oort, Pieter J.; Adams, Sean H.] USDA ARS, Western Human Nutr Res Ctr, Obes & Metab Res Unit, Davis, CA 95616 USA. [Lynch, Christopher J.; Olson, Kristine C.] Penn State Univ, Dept Cellular & Mol Physiol, Hershey, PA USA. [Mostaedi, Rouzbeh; Ali, Mohamed; Smith, William H.] Univ Calif Davis, Sch Med, Dept Surg, Davis, CA 95616 USA. [Karpe, Fredrik; Humphreys, Sandy] Univ Oxford, Oxford Ctr Diabet Endocrinol & Metab, Radcliffe Dept Med, Oxford OX1 2JD, England. [Karpe, Fredrik; Humphreys, Sandy] Oxford Univ Hosp Trust, NIHR Oxford Biomed Res Ctr, Oxford, England. [Bedinger, Daniel H.; Adams, Sean H.] Univ Calif Davis, Mol Cellular & Integrat Physiol Grad Grp, Davis, CA 95616 USA. [Bedinger, Daniel H.] Xoma Corp, Berkeley, CA 94710 USA. [Dunn, Tamara N.; Thomas, Anthony P.; Kieffer, Dorothy A.; Bettaieb, Ahmed; Haj, Fawaz G.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Dunn, Tamara N.; Thomas, Anthony P.; Kieffer, Dorothy A.; Bettaieb, Ahmed; Haj, Fawaz G.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Amin, Rajesh] Auburn State Univ, Dept Pharmacal Sci, Auburn, AL USA. [Permana, Paska] Phoenix Vet Affairs Hlth Syst, Phoenix, AZ USA. [Anthony, Tracy G.] Rutgers State Univ, Dept Nutr Sci, Rutgers, NJ USA. RP Adams, SH (reprint author), USDA ARS, Western Human Nutr Res Ctr, 430 W Hlth Sci Dr, Davis, CA 95616 USA. EM sean.h.adams@ars.usda.gov RI Amin, Raj/M-1355-2016; OI Amin, Raj/0000-0001-8741-0018; Bedinger, Daniel/0000-0001-5009-9369; Karpe, Fredrik/0000-0002-2751-1770; Anthony, Tracy/0000-0002-8586-5884 FU United States Department of Agriculture (USDA)-Agricultural Research Service (ARS) Intramural Project [5306-51530-019-00]; USDA-ARS Headquarters Postdoctoral Award; National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [R01-DK-078328]; National Dairy Council; NIDDK [R01-DK-090492] FX This research was supported by the United States Department of Agriculture (USDA)-Agricultural Research Service (ARS) Intramural Project 5306-51530-019-00, a USDA-ARS Headquarters Postdoctoral Award (D. E. Lackey), National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Grant R01-DK-078328 (S. H. Adams), and the National Dairy Council (grant administered by the Dairy Research Institute; S. H. Adams). Work in F. G. Haj's laboratory was supported by NIDDK Grant R01-DK-090492. NR 52 TC 66 Z9 69 U1 2 U2 25 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN PY 2013 VL 304 IS 11 BP E1175 EP E1187 DI 10.1152/ajpendo.00630.2012 PG 13 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 156GB UT WOS:000319808700006 PM 23512805 ER PT J AU Farmer, BD Straus, DL Beck, BH Mitchell, AJ Freeman, D Meinelt, T AF Farmer, Bradley D. Straus, David L. Beck, Benjamin H. Mitchell, Andrew J. Freeman, Donald Meinelt, Thomas TI Effectiveness of copper sulphate, potassium permanganate and peracetic acid to reduce mortality and infestation of Ichthyobodo necator in channel catfish Ictalurus punctatus (Rafinesque 1818) SO AQUACULTURE RESEARCH LA English DT Article DE effectiveness; copper sulphate; potassium permanganate; peracetic acid; Costia; I chthyobodo necator ID ICHTHYOPHTHIRIUS-MULTIFILIIS; FORMULATIONS; TOXICITY; PAA AB Ichthyobodo necator is a single-celled biflagellate parasite, which in high density can cause significant mortality in young fish. Copper sulphate (CuSO4), potassium permanganate (KMnO4) and peracetic acid (PAA) were evaluated for effectiveness against ichthyobodosis. Treatments were: untreated control, 2.1mgL-1CuSO4, 3.0mgL-1 KMnO4, 1.5mgL-1 PAA and 3.0mgL-1 PAA, and were applied to flow-through tanks on three consecutive days. The study was designed to simulate the flow-through systems utilized in the commercial rearing of juvenile channel catfish (Ictalurus punctatus). Mortality was monitored daily to compare survival rate among treatments. Parasite intensity was assessed pre chemical exposure and 20-24h after the third application to determine effectiveness of the treatment. An assessment was also done 7days post application to investigate possible reoccurrence. Copper sulphate, KMnO4 and PAA (3.0mgL-1) significantly reduced the infestation rate of I. necator. Copper sulphate significantly improved the survival of I. necator infested channel catfish after three flow-through applications compared with the untreated control. The 3.0mgL-1 PAA resulted in significantly lower survival than the untreated control, the 1.5mg L-1 PAA and the KMnO4 were not statistically different from the untreated control. C1 [Farmer, Bradley D.; Straus, David L.; Beck, Benjamin H.; Mitchell, Andrew J.; Freeman, Donald] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Meinelt, Thomas] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecophysiol & Aquaculture, Berlin, Germany. RP Farmer, BD (reprint author), USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM Bradley.Farmer@ars.usda.gov NR 24 TC 3 Z9 3 U1 2 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X J9 AQUAC RES JI Aquac. Res. PD JUN PY 2013 VL 44 IS 7 BP 1103 EP 1109 DI 10.1111/j.1365-2109.2012.03118.x PG 7 WC Fisheries SC Fisheries GA 160KR UT WOS:000320118900010 ER PT J AU Damsteegt, VD Stone, AL Smith, OP McDaniel, L Sherman, DJ Dardick, C Hammond, J Jordan, R Schneider, WL AF Damsteegt, Vernon D. Stone, Andrew L. Smith, Oney P. McDaniel, Lawrence Sherman, Diana J. Dardick, Chris Hammond, John Jordan, Ramon Schneider, William L. TI A previously undescribed potyvirus isolated and characterized from arborescent Brugmansia SO ARCHIVES OF VIROLOGY LA English DT Article ID VIRUS; INCLUSIONS; PURIFICATION; MUTATIONS; VIRIONS; PROTEIN; STRAIN; GENE AB A suspected virus disease was identified from an arborescent Brugmansia x candida Pers. (syn. Datura candida Pers.) tree. The causal agent was aphid transmissible at low rates. Viral particles were purified from infected tobacco tissue, analyzed, and purified virions were inoculated into healthy tobacco plants to recreate the symptoms. The virions had a mean length of 720-729 nm, and infected cells contained inclusion bodies typical of potyvirus infections. Analysis of infected tissues and purified virions with a panel of potyvirus-specific antibodies confirmed identification as a potyvirus. Viral host range, dilution end point, thermal tolerance and aphid transmission characteristics were examined. The viral genome (9761 nt) is typical of potyviruses, with the closest related potyvirus being pepper mottle virus, at 72 % nt sequence identity. Based on conventions for naming novel potyviruses, the virus was determined to be a member of a previously undescribed species, tentatively named "Brugmansia mosaic virus" (BruMV). C1 [Damsteegt, Vernon D.; Stone, Andrew L.; Sherman, Diana J.; Schneider, William L.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. [Smith, Oney P.] Hood Coll, Frederick, MD 21701 USA. [McDaniel, Lawrence] Amer Type Culture Collect, Manassas, VA USA. [Dardick, Chris] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [Hammond, John; Jordan, Ramon] ARS, USDA, Floral & Nursery Plants Res Unit, Beltsville, MD USA. RP Schneider, WL (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. EM william.schneider@ars.usda.gov NR 29 TC 1 Z9 1 U1 1 U2 9 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD JUN PY 2013 VL 158 IS 6 BP 1235 EP 1244 DI 10.1007/s00705-012-1600-8 PG 10 WC Virology SC Virology GA 155QK UT WOS:000319762800012 PM 23381392 ER PT J AU Tan, CT Carver, BF Chen, MS Gu, YQ Yan, LL AF Tan, Chor Tee Carver, Brett F. Chen, Ming-Shun Gu, Yong-Qiang Yan, Liuling TI Genetic association of OPR genes with resistance to Hessian fly in hexaploid wheat SO BMC GENOMICS LA English DT Article DE Hessian fly resistance; Insect resistance pathway; lipoxygenase (LOX); 12-oxophytodienoic acid reductase (OPR); Quantitative trait loci (QTL); Wheat ID TRITICUM-AESTIVUM L.; DURUM-WHEAT; CONFERRING RESISTANCE; ARABIDOPSIS-THALIANA; RUST RESISTANCE; DISTAL REGION; JASMONATE; GENOMES; MARKERS; MAP AB Background: Hessian fly (Mayetiola destructor) is one of the most destructive pests of wheat. The genes encoding 12-oxo-phytodienoic acid reductase (OPR) and lipoxygenase (LOX) play critical roles in insect resistance pathways in higher plants, but little is known about genes controlling resistance to Hessian fly in wheat. Results: In this study, 154 F-6:8 recombinant inbred lines (RILs) generated from a cross between two cultivars, 'Jagger' and '2174' of hexaploid wheat (2n = 6 x = 42; AABBDD), were used to map genes associated with resistance to Hessian fly. Two QTLs were identified. The first one was a major QTL on chromosome 1A (QHf.osu-1A), which explained 70% of the total phenotypic variation. The resistant allele at this locus in cultivar 2174 could be orthologous to one or more of the previously mapped resistance genes (H9, H10, H11, H16, and H17) in tetraploid wheat. The second QTL was a minor QTL on chromosome 2A (QHf.osu-2A), which accounted for 18% of the total phenotypic variation. The resistant allele at this locus in 2174 is collinear to an Yr17-containing-fragment translocated from chromosome 2N of Triticum ventricosum (2n = 4 x = 28; DDNN) in Jagger. Genetic mapping results showed that two OPR genes, TaOPR1-A and TaOPR2-A, were tightly associated with QHf.osu-1A and QHf.osu-2A, respectively. Another OPR gene and three LOX genes were mapped but not associated with Hessian fly resistance in the segregating population. Conclusions: This study has located two major QTLs/genes in bread wheat that can be directly used in wheat breeding programs and has also provided insights for the genetic association and disassociation of Hessian fly resistance with OPR and LOX genes in wheat. C1 [Tan, Chor Tee; Carver, Brett F.; Yan, Liuling] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Gu, Yong-Qiang] USDA, ARS, Genom & Gene Discovery Res Unit, Albany, CA 94710 USA. RP Yan, LL (reprint author), Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. EM liuling.yan@okstate.edu FU USDA-NIFA T-CAP [2011-68002-30029]; Oklahoma Center of Advanced Science and Technology (OCAST) [PAS07-002]; Oklahoma Wheat Research Foundation FX This work was supported by USDA-NIFA T-CAP grant No. 2011-68002-30029, the Oklahoma Center of Advanced Science and Technology (OCAST, PAS07-002), and the Oklahoma Wheat Research Foundation. NR 69 TC 6 Z9 6 U1 3 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 1 PY 2013 VL 14 AR 369 DI 10.1186/1471-2164-14-369 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 160KE UT WOS:000320117600001 PM 23724909 ER PT J AU Kim, EK Kim, YS Milner, JA Wang, TTY AF Kim, Eun-Kyung Kim, Young S. Milner, John A. Wang, Thomas T. Y. TI Indole-3-Carbinol and 3 ',3 '-Diindolylmethane Modulate Androgen's Effect on C-C Chemokine Ligand 2 and Monocyte Attraction to Prostate Cancer Cells SO CANCER PREVENTION RESEARCH LA English DT Article ID HUMAN BREAST-CANCER; EPITHELIAL-MESENCHYMAL TRANSITION; STEM-CELLS; TWIST; INFLAMMATION; EXPRESSION; RECEPTOR; CCL2; CHEMOATTRACTANT; CARCINOGENESIS AB Inflammation has a role in prostate tumorigenesis. Recruitment of inflammatory monocytes to the tumor site is mediated by C-C chemokine ligand 2 (CCL2) through binding to its receptor CCR2. We hypothesized that androgen could modulate CCL2 expression in hormone-responsive prostate cancer cells and thereby promote recruitment of monocytes. Given the inhibitory effect of broccoli-derived compounds indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM) on androgen-dependent pathways, we also reasoned that I3C and DIM could modulate the effect of androgen on CCL2-mediated pathways. Dihydrotestosterone was found to induce a time-dependent (0-72 hours) and concentration-dependent (0-1 nmol/L) increase in CCL2 mRNA levels in androgen-responsive human prostate cancer cells (LNCaP). This increase in CCL2 mRNA corresponded with increased secretion of CCL2 protein. The effect of dihydrotestosterone was mediated through an androgen receptor (AR)-dependent pathway as small inhibitor RNA against AR negated the induction of CCL2. Although dihydrotestosterone also induced TWIST1 mRNA, an epithelial-mesenchymal transition-related factor, and purported inducer of CCL2, blocking its expression with small inhibitor RNA did not inhibit dihydrotestosterone induction of CCL2 mRNA. Moreover, conditioned media from androgen-treated cells promoted human monocyte THP-1 cell migration and this effect was blocked by antibody against CCL-2. Both I3C and DIM inhibited promotional effects of dihydrotestosterone on CCL2 and migration. These results show that androgen may regulate CCL2 and promote inflammatory microenvironment in prostate tumors and that this process can be blocked by broccoli-derived compounds. (C) 2013 AACR. C1 [Kim, Eun-Kyung; Kim, Young S.; Milner, John A.] NCI, Nutr Sci Res Grp, Canc Prevent Div, NIH, Bethesda, MD 20892 USA. [Kim, Eun-Kyung] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Kim, Eun-Kyung; Wang, Thomas T. Y.] ARS, Diet Gen & Immunol Lab, USDA, Beltsville, MD 20705 USA. [Milner, John A.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Wang, TTY (reprint author), USDA, Bldg 307C,Room 132,BARC EAST,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM tom.wang@ars.usda.gov FU U.S appropriated funds [1235-51530-053-00D]; National Cancer Institute FX This work was supported by U.S appropriated funds to USDA project number 1235-51530-053-00D (to T.T.Y. Wang) and the National Cancer Institute (to E.-K. Kim, Y.S. Kim, J.A. Milner). NR 49 TC 3 Z9 3 U1 1 U2 4 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1940-6207 J9 CANCER PREV RES JI Cancer Prev. Res. PD JUN PY 2013 VL 6 IS 6 BP 519 EP 529 DI 10.1158/1940-6207.CAPR-12-0419 PG 11 WC Oncology SC Oncology GA 156GR UT WOS:000319810400003 PM 23585426 ER PT J AU Westfall, JA Woodall, CW Hatfield, MA AF Westfall, James A. Woodall, Christopher W. Hatfield, Mark A. TI A statistical power analysis of woody carbon flux from forest inventory data SO CLIMATIC CHANGE LA English DT Article ID DISTURBANCES AB At a national scale, the carbon (C) balance of numerous forest ecosystem C pools can be monitored using a stock change approach based on national forest inventory data. Given the potential influence of disturbance events and/or climate change processes, the statistical detection of changes in forest C stocks is paramount to maintaining the net sequestration status of these stocks. To inform the monitoring of forest C balances across large areas, a power analysis of a forest inventory of live/dead standing trees and downed dead wood C stocks (and components thereof) was performed in states of the Great Lakes region, U.S. Using data from the Forest Inventory and Analysis (FIA) program of the U.S. Forest Service, it was found that a decrease in downed wood C stocks (-1.87 Mg/ha) was nearly offset by an increase in standing C stocks (1.77 Mg/ha) across the study region over a 5-year period. Carbon stock change estimates for downed dead wood and standing pools were statistically different from zero (alpha = 0.10), while the net change in total woody C (-0.10 Mg/ha) was not statistically different from zero. To obtain a statistical power to detect change of 0.80 (alpha = 0.10), standing live C stocks must change by at least 0.7 %. Similarly, standing dead C stocks would need to change by 3.8 %; while downed dead C stocks require a change of 6.9 %. While the U.S.'s current forest inventory design and sample intensity may not be able to statistically detect slight changes (< 1 %) in forest woody C stocks at sub-national scales, large disturbance events (> 3 % stock change) would almost surely be detected. Understanding these relationships among change detection thresholds, sampling effort, and Type I (alpha) error rates allows analysts to evaluate the efficacy of forest inventory data for C pool change detection at various spatial scales and levels of risk for drawing erroneous conclusions. C1 [Westfall, James A.] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA USA. [Woodall, Christopher W.; Hatfield, Mark A.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. RP Westfall, JA (reprint author), US Forest Serv, USDA, No Res Stn, Newtown Sq, PA USA. EM jameswestfall@fs.fed.us OI Woodall, Christopher/0000-0001-8076-6214 NR 25 TC 5 Z9 5 U1 0 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 J9 CLIMATIC CHANGE JI Clim. Change PD JUN PY 2013 VL 118 IS 3-4 BP 919 EP 931 DI 10.1007/s10584-012-0686-z PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 150VB UT WOS:000319418300031 ER PT J AU Canning, P AF Canning, Patrick TI MAXIMUM-LIKELIHOOD ESTIMATES OF A US MULTIREGIONAL HOUSEHOLD EXPENDITURE SYSTEM SO ECONOMIC SYSTEMS RESEARCH LA English DT Article DE Constrained maximum likelihood; Household expenditures; Hypothesis testing; Multiregional; Sufficient statistics ID LEAST-SQUARES; INFORMATION; ACCOUNTS AB This paper applies the maximum-likelihood equation to a model that produces US regional household expenditure estimates using national-level data on average expenditures by type of household and regional data on the number of households by type. Empirical results follow the analytical properties of the model and demonstrate an impressive capacity to recover regional statistics. These findings are useful in applied regional studies since they demonstrate a general framework to assess the input data and the overall estimation model. C1 USDA, Econ Res Serv, Washington, DC 20250 USA. RP Canning, P (reprint author), USDA, Econ Res Serv, Washington, DC 20250 USA. EM pcanning@ers.usda.gov NR 29 TC 1 Z9 1 U1 0 U2 1 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0953-5314 J9 ECON SYST RES JI Econ. Syst. Res. PD JUN 1 PY 2013 VL 25 IS 2 BP 245 EP 264 DI 10.1080/09535314.2012.730991 PG 20 WC Economics SC Business & Economics GA 159YL UT WOS:000320083100006 ER PT J AU Yu, JW Yu, SX Gore, M Wu, M Zhai, HH Li, XL Fan, SL Song, MZ Zhang, JF AF Yu, Jiwen Yu, Shuxun Gore, Michael Wu, Man Zhai, Honghong Li, Xingli Fan, Shuli Song, Meizhen Zhang, Jinfa TI Identification of quantitative trait loci across interspecific F-2, F-2:3 and testcross populations for agronomic and fiber traits in tetraploid cotton SO EUPHYTICA LA English DT Article DE Tetraploid cotton; Linkage map; Testcross; Quantitative trait loci (QTL) ID X GOSSYPIUM-BARBADENSE; BACKCROSS-SELF APPROACH; MOLECULAR DISSECTION; RESISTANCE GENES; GENOME STRUCTURE; ARID CONDITIONS; G. BARBADENSE; UPLAND COTTON; QTL ANALYSIS; LINKAGE MAP AB The most widely grown tetraploid Gossypium hirsutum and G. barbadense differ greatly in yield potential and fiber quality and numerous quantitative trait loci (QTLs) have been reported. However, correspondence of QTLs between experiments and populations is poor due to limited number of markers, small population size and inaccurate phenotyping. The purpose of the present study was to map QTLs for yield, yield components and fiber quality traits using testcross progenies between a large interspecific F-2 population and a commercial cotton cultivar as the tester. The results were compared to these from its F-2 and F-2:3 progenies. Of the 177 QTLs identified from the three populations, 65 fiber QTLs and 51 yield QTLs were unique with an average of 8-12 QTLs per traits. All the 26 chromosomes carried QTLs, but differed in the number of QTLs and the number of QTLs between fiber and yield QTLs. The congruence of QTLs identified across populations was higher (20-60 %) for traits with higher heritabilities including fiber quality, seed index and lint percentage, but lower (10-25 %) for lower heritability traits-seedcotton and lint yields. Major QTLs, QTL clusters for the same traits and QTL 'hotspots' for different traits were also identified. This research represents the first report using a testcross population in QTL mapping in interspecific cotton crosses and provides useful information for further comparative analysis and marker-assisted selection. C1 [Yu, Jiwen; Yu, Shuxun; Wu, Man; Zhai, Honghong; Li, Xingli; Fan, Shuli; Song, Meizhen] Chinese Acad Agr Sci, Cotton Res Inst, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. [Gore, Michael] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Zhang, Jinfa] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. RP Yu, SX (reprint author), Chinese Acad Agr Sci, Cotton Res Inst, State Key Lab Cotton Biol, Anyang 455000, Henan, Peoples R China. EM yujw666@hotmail.com; Michael.Gore@ars.usda.gov; jinzhang@nmsu.edu FU National Basic Research Program of China ("973" Program) [2010CB126006]; National High Technology Research and Development Program of China ("863" Program) [2012AA101108]; New Mexico Agricultural Experiment Station, New Mexico, USA; USDA-ARS (M.A.G.) FX This work was supported by a Grant from the National Basic Research Program of China (i.e. "973" Program) (No. 2010CB126006) and the National High Technology Research and Development Program of China (i.e. "863" Program) (No. 2012AA101108); and the New Mexico Agricultural Experiment Station, New Mexico, USA. This research was supported in part by USDA-ARS (M.A.G.). Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is equal opportunity provider and employer. NR 39 TC 17 Z9 19 U1 4 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUN PY 2013 VL 191 IS 3 BP 375 EP 389 DI 10.1007/s10681-013-0875-5 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 155NJ UT WOS:000319754800005 ER PT J AU Fang, DD Hinze, LL Percy, RG Li, P Deng, D Thyssen, G AF Fang, David D. Hinze, Lori L. Percy, Richard G. Li, Ping Deng, Dewayne Thyssen, Gregory TI A microsatellite-based genome-wide analysis of genetic diversity and linkage disequilibrium in Upland cotton (Gossypium hirsutum L.) cultivars from major cotton-growing countries SO EUPHYTICA LA English DT Article DE Upland cotton (Gossypium hirsutum); Microsatellite markers; Linkage disequilibrium; Genetic diversity; Cultivar characterization ID SIMPLE SEQUENCE REPEAT; FRAGMENT-LENGTH-POLYMORPHISMS; MOLECULAR MARKERS; FIELD PERFORMANCE; SSR MARKERS; GERMPLASM; MAP; TRANSFORMATION; CONSTRUCTION; TECHNOLOGY AB To better understand the genetic diversity of the cultivated Upland cotton (Gossypium hirsutum L.) and its structure at the molecular level, 193 Upland cotton cultivars collected from 26 countries were genotyped using 448 microsatellite markers. These markers were selected based on their mapping positions in the high density G. hirsutum TM-1 x G. barbadense 3-79 map, and they covered the whole genome. In addition, the physical locations of these markers were also partially identified based on the reference sequence of the diploid G. raimondii (D-5) genome. The marker orders in the genetic map were largely in agreement with their orders in the physical map. These markers revealed 1,590 alleles belonging to 732 loci. Analysis of unique marker allele numbers indicated that the modern US Upland cotton had been losing its genetic diversity during the past century. Linkage disequilibrium (LD) between marker pairs was clearly un-even among chromosomes, and among regions within a chromosome. The average size of a LD block was 6.75 cM at r (2) = 0.10. A neighbor-joining phylogenic tree of these cultivars was generated using marker allele frequencies based on Nei's genetic distance. The cultivars were grouped into 15 groups according to the phylogenic tree. Grouping results were largely congruent with the breeding history and pedigrees of the cultivars with a few exceptions. C1 [Fang, David D.; Li, Ping; Thyssen, Gregory] ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, New Orleans, LA 70124 USA. [Hinze, Lori L.; Percy, Richard G.] ARS, Crop Germplasm Res Unit, USDA, SPARC, College Stn, TX 77845 USA. [Deng, Dewayne] ARS, Genet & Precis Agr Res Unit, USDA, Mississippi, MS 39762 USA. RP Fang, DD (reprint author), ARS, Cotton Fiber Biosci Res Unit, USDA, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459; Hinze, Lori L/0000-0003-3356-1245 FU Cotton Incorporated FX This project was partially supported by Cotton Incorporated. We thank Dr. Linghe Zeng at USDA-ARS, Stoneville, MS for providing us seeds of some cultivars used in this study. Our great appreciation goes to Mrs. Sheron Simpson and Dr. Brian Scheffler at Genomic and Bioinformatic Research Unit at Stoneville, MS for their excellent support in SSR marker analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture that is an equal opportunity provider and employer. NR 48 TC 31 Z9 34 U1 1 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUN PY 2013 VL 191 IS 3 BP 391 EP 401 DI 10.1007/s10681-013-0886-2 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 155NJ UT WOS:000319754800006 ER PT J AU Gurtler, JB Marks, HM Bailey, RB Juneja, V Jones, DR AF Gurtler, Joshua B. Marks, Harry M. Bailey, Rebecca B. Juneja, Vijay Jones, Deana R. TI Kinetics Model Comparison for the Inactivation of Salmonella Serotypes Enteritidis and Oranienburg in 10% Salted Liquid Whole Egg SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID THERMAL-RESISTANCE; LISTERIA-MONOCYTOGENES; YOLK PRODUCTS; PASTEURIZATION; DESTRUCTION; TEMPERATURES; CURVES AB The goal of this study was to determine the inactivation kinetics of Salmonella in commercial 10% salted liquid whole egg (LWE) to assist the U.S. Department of Agriculture in writing new liquid egg pasteurization guidelines. Current data are not sufficient for predicting thermal inactivation kinetics of Salmonella spp. for use in updating pasteurization guidelines for many types of liquid egg products, including salted LWE (SLWE). This is, in part, due to variations in Salmonella strains and changes in the processing of liquid egg products that have arisen in the past 40 years. Pasteurization guidelines are currently being reevaluated in light of recent risk assessments. Heat-resistant Salmonella serovars Enteritidis and Oranienburg were composited and mixed into 10% SLWE, resulting in final populations of approximately 5.7-7.8 log colony-forming units (CFU)/mL. Inoculated egg was injected into glass capillary tubes, flame-sealed, and heated in a water bath at 60, 62.2, 63.3, 64.3, or 66 degrees C. Contents were surface-plated and incubated at 37 degrees C for 24 h. Survival curves were not log-linear (log levels versus time), but decreased rapidly, and after initial periods became linear. Asymptotic decimal reduction values at each temperature were calculated from survivor curves with a minimum inactivation of 5.0 log CFU/mL. The asymptotic thermal D-values for SLWE were 3.47, 2.23, 1.79, 1.46, and 1.04 min at 60, 62.2, 63.3, 64.3, or 66 degrees C, respectively. The calculated thermal z-value was 11.5 degrees C. A model that predicts lethality for given times and temperatures that was developed predicted that the current pasteurization requirements for 10% SLWE (i.e., 63.3 degrees C for 3.5 min, or 62.2 degrees C for 6.2 min) are not sufficient to inactivate 7 log CFU/mL of Salmonella and only achieve approximately 4 log CFU/mL inactivation. This model will assist egg-products manufacturers and regulatory agencies in designing pasteurization processes to ensure product safety. C1 [Gurtler, Joshua B.; Bailey, Rebecca B.] USDA ARS, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Juneja, Vijay] Eastern Reg Res Ctr, Residue Chem & Predict Microbiol Res Unit, Wyndmoor, PA 19038 USA. [Marks, Harry M.] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Jones, Deana R.] USDA ARS, Richard B Russell Agr Res Ctr, Egg Safety & Qual Res Unit, Athens, GA 30613 USA. RP Gurtler, JB (reprint author), USDA ARS, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM joshua.gurtler@ars.usda.gov FU USDA, FSIS FX The authors thank USDA reviewers, Allen Sheen and Dike Ukuku, for proofreading. Liquid egg products were provided by Michael Foods, Gaylord, MN. Technical assistance was provided by Patsy Mason and Victoria Broussard. This research was funded based on needs prioritized by the USDA, FSIS. Special thanks to DVM Kristin G. Holt, USDA, FSIS, OPHS, OAA, liaison to CDC and Karen M. Herman, CDC Enteric Diseases Epidemiology Branch, for assistance with CDC foodborne outbreak information. NR 34 TC 2 Z9 2 U1 0 U2 15 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2013 VL 10 IS 6 BP 492 EP 499 DI 10.1089/fpd.2012.1366 PG 8 WC Food Science & Technology SC Food Science & Technology GA 164AC UT WOS:000320380100002 PM 23763579 ER PT J AU Gorski, L Jay-Russell, MT Liang, AS Walker, S Bengson, Y Govoni, J Mandrell, RE AF Gorski, Lisa Jay-Russell, Michele T. Liang, Anita S. Walker, Samarpita Bengson, Yingjia Govoni, Jessica Mandrell, Robert E. TI Diversity of Pulsed-Field Gel Electrophoresis Pulsotypes, Serovars, and Antibiotic Resistance Among Salmonella Isolates from Wild Amphibians and Reptiles in the California Central Coast SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID ESCHERICHIA-COLI O157-H7; ENTERICA SUBSP ARIZONAE; RESERVOIR; TYPHIMURIUM; CULTIVATION; STRAINS; O157H7; INFANT; CATTLE; WATER AB A survey of cold-blooded vertebrates and associated surface waters in a produce-growing region on the Central California Coast was done between May and September 2011 to determine the diversity of Salmonella. Samples from 460 amphibians and reptiles and 119 water samples were collected and cultured for Salmonella. Animals sampled were frogs (n = 331), lizards (n = 59), newts (n = 5), salamanders (n = 6), snakes (n = 39), and toads (n = 20). Salmonella was isolated from 37 individual animals, including frogs, lizards, snakes, and toads. Snakes were the most likely to contain Salmonella, with 59% testing positive followed by 15.3% of lizards, 5% of toads, and 1.2% of frogs. Fifteen water samples (12.6%) were positive. Twenty-two different serovars were identified, and the majority of isolates were S. enterica subsp. IIIb, with subsp. I, II, and IIIa also found. The serovar isolated most frequently was S. enterica subsp. IIIb 16: z(10):e,n,x,z(15), from snakes and frogs in five different locations. S. enterica subsp. I serovar Typhimurium and the monophasic I 6,8:d:- were isolated from water, and subspecies I Duisburg and its variants were found in animals and water. Some samples contained more than one type of Salmonella. Analysis of pulsed-field gel electrophoresis pulsotypes indicated that some strains persisted in animals and water collected from the same location. Sixty-six isolates displayed antibiotic resistance, with 27 isolates resistant to more than one antibiotic, including a subspecies IIIb isolate from snake having resistance to five different antibiotics. Twenty-three isolates were resistant to more than one class of antibiotic, and six isolates were resistant to three classes. While these subspecies of IIIa and IIIb cause fewer instances of human illness, they may serve as reservoirs of antibiotic resistance, determinants in the environment, and be sources of contamination of leafy greens associated with product recalls. C1 [Gorski, Lisa; Liang, Anita S.; Walker, Samarpita; Govoni, Jessica; Mandrell, Robert E.] ARS, USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. [Jay-Russell, Michele T.; Bengson, Yingjia] Univ Calif Davis, Western Ctr Food Safety, Davis, CA 95616 USA. RP Gorski, L (reprint author), ARS, USDA, Produce Safety & Microbiol Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM lisa.gorski@ars.usda.gov FU Center for Produce Safety through the California Department of Food and Agriculture, Specialty Crop Block Grant [SCB10056]; U. S. Food and Drug Administration [U01-003-572]; U.S. Department of Agriculture, Agricultural Research Service CRIS project [5325-42000-046-00D] FX We are grateful to Dawn Reis, Jessica Wheeler, Jordi Montfort, Alexis Fisher, Stephanie Huang, Diana Carychao, and Kimberly Nguyen for technical assistance. We also extend our thanks to the growers and property owners who kindly allowed access to their farms. Funding for this project was provided by the Center for Produce Safety through the California Department of Food and Agriculture, Specialty Crop Block Grant #SCB10056, the U. S. Food and Drug Administration (Project No. U01-003-572), and U.S. Department of Agriculture, Agricultural Research Service CRIS project 5325-42000-046-00D. NR 39 TC 8 Z9 8 U1 2 U2 17 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD JUN PY 2013 VL 10 IS 6 BP 540 EP 548 DI 10.1089/fpd.2012.1372 PG 9 WC Food Science & Technology SC Food Science & Technology GA 164AC UT WOS:000320380100009 PM 23577627 ER PT J AU Mehl, HK Mori, SR Frankel, SJ Rizzo, DM AF Mehl, H. K. Mori, S. R. Frankel, S. J. Rizzo, D. M. TI Mortality and growth of dwarf mistletoe-infected red and white fir and the efficacy of thinning for reducing associated losses SO FOREST PATHOLOGY LA English DT Article ID WESTERN HEMLOCK; DOUGLAS-FIR; CALIFORNIA; PARASITISM AB In managed forests dominated by true fir (Abies) species, stands are often restocked using understory trees retained during timber harvest, making the effects of dwarf mistletoe (Arceuthobium spp.) infestation on small true fir a concern. This study examined the response of small red (A.magnifica) and white (A.concolor) fir and their dwarf mistletoes (A.abietinum f.sp.magnificae and A.abietinum f.sp.concoloris, respectively) to precommercial thinning treatments in fir-dominated stands in the Sierra Nevada Mountains of California. Tree diameters and dwarf mistletoe ratings were monitored from 1981 to 2001, and mortality was observed from 1981 to 2006. Red and white fir survival and radial growth decreased significantly with greater mistletoe ratings and increased with larger diameters and live crown ratios. Thinning significantly increased survival and growth of white, but not red fir. Over the course of the study, mistletoe ratings for both fir species did not change significantly in unthinned stands, but increased in thinned stands. However, while statistically significant, increases in mistletoe ratings in thinned stands were gradual and mistletoe ratings did not statistically differ between treatments 20years post-thinning. Additionally, thinning did not significantly influence mistletoe spread to uninfected trees, indicating that precommercial thinning in lightly infested red and white fir stands will not likely result in substantial increases in dwarf mistletoe abundance over typical harvesting intervals. Overall, while severe dwarf mistletoe infection ratings reduced tree survival and growth, because ratings remained low, actual losses resulting from mistletoes during this study were minor and will not likely result in substantial economic losses over normal harvesting intervals. This finding indicates that precommercial thinning treatments designed specifically to compensate for mistletoe-associated losses may not be necessary when managing red and white fir for timber production. C1 [Mehl, H. K.; Rizzo, D. M.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Mori, S. R.; Frankel, S. J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA USA. RP Mehl, HK (reprint author), Univ Calif Davis, Dept Plant Pathol, 1 Shields Ave, Davis, CA 95616 USA. EM hkmehl@ucdavis.edu FU USDA Forest Service, Pacific Southwest Research Station, Pacific Southwest Region FX We would like to thank the original cooperators who established this project: Robert F. Scharpf, Richard S. Smith and Detlev R. Vogler. We would also like to thank Don Triplat, Gary Fiddler, John Kliejunas and Garey W. Slaughter for contributions to plot remeasurements and Beverly Bualon for data stewardship. Support for project establishment and data collection came from the USDA Forest Service, Pacific Southwest Research Station, Pacific Southwest Region, and support for data analysis came from USDA Forest Service, Forest Health Monitoring, Pest Trend Impact Plots: Analysis and Extension (WC-AR-09-03). We would also like to thank three anonymous reviewers for insightful comments, Richard Cobb and Margaret Metz for comments on earlier drafts of the manuscript and Clay DeLong and Matthew Hamilton for assistance with maps and figures. NR 55 TC 1 Z9 1 U1 4 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 J9 FOREST PATHOL JI Forest Pathol. PD JUN PY 2013 VL 43 IS 3 BP 193 EP 203 DI 10.1111/efp.12020 PG 11 WC Forestry SC Forestry GA 160RU UT WOS:000320137900003 ER PT J AU Jacobi, WR Koski, RD Negron, JF AF Jacobi, W. R. Koski, R. D. Negron, J. F. TI Dutch elm disease pathogen transmission by the banded elm bark beetle Scolytus schevyrewi SO FOREST PATHOLOGY LA English DT Article ID COLEOPTERA-SCOLYTIDAE; MULTISTRIATUS; ULMI; VECTORS AB Dutch Elm Disease (DED) is a vascular wilt disease of Ulmus species (elms) incited in North America primarily by the exotic fungus Ophiostoma novo-ulmi. The pathogen is transmitted via root grafts and elm bark beetle vectors, including the native North American elm bark beetle, Hylurgopinus rufipes and the exotic smaller European elm bark beetle, Scolytus multistriatus. The banded elm bark beetle, Scolytus schevyrewi, is an exotic Asian bark beetle that is now apparently the dominant elm bark beetle in the Rocky Mountain region of the USA. It is not known if S.schevyrewi will have an equivalent vector competence or if management recommendations need to be updated. Thus the study objectives were to: (i) determine the type and size of wounds made by adult S.schevyrewi on branches of Ulmus americana and (ii) determine if adult S.schevyrewi can transfer the pathogen to American elms during maturation feeding. To determine the DED vectoring capability of S.schevyrewi, newly emerged adults were infested with spores of Ophiostoma novo-ulmi and then placed with either in-vivo or in-vitro branches of American elm trees. The inoculation of trees via feeding wounds was successful 30% of the time for in-vivo trials and 33% for in-vitro trials. Although the infection rate of DED has declined in Colorado over the past 10years, the disease is still present in urban elms. While it appears that S.schevyrewi is another vector of the DED pathogens, it appears that S.schevyrewi is no more efficient than S.multistriatus. Thus, management programs that remove elm bark beetle breeding sites, rapidly remove DED-infected elms and include the planting of DED-resistant elms should continue to be effective management tactics. C1 [Jacobi, W. R.; Koski, R. D.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Negron, J. F.] US Forest Serv, USDA, Rocky Mt Forest Res Stn, Ft Collins, CO USA. RP Jacobi, WR (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM william.jacobi@colostate.edu FU John Z. Duling research grant of the Tree Fund; USDA Forest Service, Forest Health Protection, Special Technology Development Program; USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO; Colorado State Agricultural Research Station, Colorado State University, Fort Collins CO FX We appreciate the technical assistance by Silvia Gomez Fernandez, research advice by Whitney Cranshaw, Jeff Witcosky, John Popp, and Thomas Harrington. We appreciate the suggested improvements to the manuscript by anonymous reviewers and of previous versions by Janet Hardin, Ned Tisserat and Howard Schwartz. Funding was provided by the John Z. Duling research grant of the Tree Fund, the USDA Forest Service, Forest Health Protection, Special Technology Development Program, the USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO and the Colorado State Agricultural Research Station, Colorado State University, Fort Collins CO. NR 23 TC 7 Z9 9 U1 6 U2 71 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 J9 FOREST PATHOL JI Forest Pathol. PD JUN PY 2013 VL 43 IS 3 BP 232 EP 237 DI 10.1111/efp.12023 PG 6 WC Forestry SC Forestry GA 160RU UT WOS:000320137900007 ER PT J AU Riedelsheimer, C Endelman, JB Stange, M Sorrells, ME Jannink, JL Melchinger, AE AF Riedelsheimer, Christian Endelman, Jeffrey B. Stange, Michael Sorrells, Mark E. Jannink, Jean-Luc Melchinger, Albrecht E. TI Genomic Predictability of Interconnected Biparental Maize Populations SO GENETICS LA English DT Article ID GENETIC-RELATIONSHIP INFORMATION; HEAD BLIGHT RESISTANCE; GIBBERELLA EAR ROT; BREEDING VALUES; QUANTITATIVE TRAITS; GENOMEWIDE SELECTION; HYBRID PERFORMANCE; PREDICTION MODELS; RIDGE-REGRESSION; COMPLEX TRAITS AB Intense structuring of plant breeding populations challenges the design of the training set (TS) in genomic selection (GS). An important open question is how the TS should be constructed from multiple related or unrelated small biparental families to predict progeny from individual crosses. Here, we used a set of five interconnected maize (Zea mays L.) populations of doubled-haploid (DH) lines derived from four parents to systematically investigate how the composition of the TS affects the prediction accuracy for lines from individual crosses. A total of 635 DH lines genotyped with 16,741 polymorphic SNPs were evaluated for five traits including Gibberella ear rot severity and three kernel yield component traits. The populations showed a genomic similarity pattern, which reflects the crossing scheme with a clear separation of full sibs, half sibs, and unrelated groups. Prediction accuracies within full-sib families of DH lines followed closely theoretical expectations, accounting for the influence of sample size and heritability of the trait. Prediction accuracies declined by 42% if full-sib DH lines were replaced by half-sib DH lines, but statistically significantly better results could be achieved if half-sib DH lines were available from both instead of only one parent of the validation population. Once both parents of the validation population were represented in the TS, including more crosses with a constant TS size did not increase accuracies. Unrelated crosses showing opposite linkage phases with the validation population resulted in negative or reduced prediction accuracies, if used alone or in combination with related families, respectively. We suggest identifying and excluding such crosses from the TS. Moreover, the observed variability among populations and traits suggests that these uncertainties must be taken into account in models optimizing the allocation of resources in GS. C1 [Riedelsheimer, Christian; Stange, Michael; Melchinger, Albrecht E.] Univ Hohenheim, Inst Plant Breeding Seed Sci & Populat Genet, D-70593 Stuttgart, Germany. [Endelman, Jeffrey B.; Sorrells, Mark E.; Jannink, Jean-Luc] Cornell Univ, RW Holley Ctr Agr & Hlth, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Jannink, Jean-Luc] Cornell Univ, RW Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. RP Melchinger, AE (reprint author), Univ Hohenheim, Inst Plant Breeding Seed Sci & Populat Genet, D-70593 Stuttgart, Germany. EM melchinger@uni-hohenheim.de FU German Federal Ministry of Education and Research [0315528D]; Deutsche Forschungsgemeinschaft [ME 2260/6-1]; Bill and Melinda Gates Foundation FX This research was funded by the German Federal Ministry of Education and Research within the Agro-ClustEr "Synbreed-Synergistic plant and animal breeding" (grant 0315528D) and by the Deutsche Forschungsgemeinschaft research grant ME 2260/6-1. Funding to J.L.J. and M.E.S. in support of this research came from a Bill and Melinda Gates Foundation grant "Genomic selection: the next frontier for rapid genetic gains in maize and wheat." NR 50 TC 46 Z9 46 U1 1 U2 40 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 1943-2631 J9 GENETICS JI Genetics PD JUN PY 2013 VL 194 IS 2 BP 493 EP + DI 10.1534/genetics.113.150227 PG 25 WC Genetics & Heredity SC Genetics & Heredity GA 156IG UT WOS:000319814700017 PM 23535384 ER PT J AU Beck, PA Anders, M Watkins, B Gunter, SA Hubbell, D Gadberry, MS AF Beck, P. A. Anders, M. Watkins, B. Gunter, S. A. Hubbell, D. Gadberry, M. S. TI 2011 AND 2012 EARLY CAREERS ACHIEVEMENT AWARDS: Improving the production, environmental, and economic efficiency of the stocker cattle industry in the southeastern United States SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE economics; growing cattle; pasture; runoff; soil quality ID ANIMAL PERFORMANCE; FORAGE; PASTURES; SYSTEMS; RUNOFF; STEERS; SOIL AB Grazing forages on small-grain fields can be a profitable "second crop" for grain producers and an opportunity for cow-calf producers to retain ownership of weaned calves. The increasing costs of conventional tillage and movement of soil nutrients into surface water creates a need for more sustainable production practices to be incorporated by producers into wheat pasture production systems. Research at the Livestock and Forestry Research Station near Batesville, AR, and the Southwest Research and Extension Center near Hope, AR, has been conducted over a 9-yr span to characterize the impacts of pasture systems on forage production, animal performance, soil quality, water runoff, and the economics associated with the stocker cattle enterprises. Gains of growing cattle grazing nontoxic endophyte-infected tall fescue and small-grain forages can be increased by 80 and 150%, respectively, compared with grazing Bermuda grass or toxic endophyte-infected tall fescue. Producers grazing spring-calving cowherds can use these improved forages to accelerate stocker performance when retaining calves in the fall and improve net returns by 99% with winter annual or nontoxic tall fescue production systems compared with Bermuda grass or toxic tall fescue. Rainfall simulation of small grain pastures indicates that runoff volume and nutrient load does not differ between conventionally tilled fields and no-till fields in the spring before tillage when soil surface cover is similar. In the fall after tillage, however, conventionally tilled fields had 4 times greater runoff; hence, there was 1.9 times greater N runoff and 3.2 times greater P runoff in conventionally tilled fields compared with no-till. Total natural rainfall runoff from conventionally tilled wheat fields were 2 times greater than from no-till fields with 25 mm rainfall events yet were 4 times greater with 62-mm rainfall events. Soil analysis shows that soil aggregate content was greater in no-till compared with conventional till, indicating greater soil porosity, improved water infiltration rate, and reduced erositivity of soil. Carbon concentration in no-till soils was 50% greater than conventional tillage after 9 yr. These experiments show that production systems can be designed that maintain livestock production, increase soil quality, reduce nutrient discharge, and promote improved economic returns. C1 [Beck, P. A.] Univ Arkansas, Div Agr, Southwest Res & Extens Ctr, Hope, AR 71801 USA. [Anders, M.; Watkins, B.] Univ Arkansas, Div Agr, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Gunter, S. A.] USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. [Hubbell, D.] Univ Arkansas, Div Agr, Livestock & Forestry Res Stn, Batesville, AR 72501 USA. [Gadberry, M. S.] Univ Arkansas, Cooperat Extens Serv, Little Rock, AR 72204 USA. RP Beck, PA (reprint author), Univ Arkansas, Div Agr, Southwest Res & Extens Ctr, Hope, AR 71801 USA. EM pbeck@uaex.edu FU Journal of Animal Science; American Society of Animal Science; American Society of Animal Science Foundation; National Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture (NIFA) [2005-35101-15344]; Univ. of Arkansas Agric. Exp. Sta. [AR002159]; NIFA FX Based on a presentation by P. A. Beck, as a recipient of an ASAS Early Career Achievement Award, at the Joint Annual Meeting, July 15-19, 2012, Phoenix, Arizona, with publication sponsored by the Journal of Animal Science, the American Society of Animal Science, and American Society of Animal Science Foundation.; This project was partially supported by National Research Initiative Competitive Grant No. 2005-35101-15344 from the USDA National Institute of Food and Agriculture (NIFA) and by Hatch Project no. AR002159 from the Univ. of Arkansas Agric. Exp. Sta. and NIFA. NR 33 TC 7 Z9 7 U1 0 U2 13 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2456 EP 2466 DI 10.2527/jas.2012-5873 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200002 PM 23243161 ER PT J AU Meyer, AM Neville, TL Reed, JJ Taylor, JB Reynolds, LP Redmer, DA Hammer, CJ Vonnahme, KA Caton, JS AF Meyer, A. M. Neville, T. L. Reed, J. J. Taylor, J. B. Reynolds, L. P. Redmer, D. A. Hammer, C. J. Vonnahme, K. A. Caton, J. S. TI Maternal nutritional plane and selenium supply during gestation impact visceral organ mass and intestinal growth and vascularity of neonatal lamb offspring SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE development; fetal programming; gastrointestinal tract; growth; neonate; pregnancy ID PREGNANT EWE LAMBS; FETAL BODY-WEIGHT; GASTROINTESTINAL-TRACT; CELL-PROLIFERATION; NUTRIENT RESTRICTION; JEJUNAL VASCULARITY; DIETARY RESTRICTION; ANGIOGENIC FACTORS; PRIMIPAROUS EWES; SUPPLEMENTATION AB To investigate effects of nutritional plane and Se supply during gestation on neonatal offspring visceral organ mass and intestinal growth and vascularity, 84 nulliparous Rambouillet ewes (age = 240 +/- 17 d, BW = 52.1 +/- 6.2 kg) were allocated to a 2 x 3 factorial design. Ewes were fed 1 of 2 Se diets [adequate Se (ASe, 11.5 mu g/kg BW) or high Se (HSe, 77.0 mu g/kg BW)], initiated at breeding, and 1 of 3 nutritional planes [60% (restricted; RES), 100% (control; CON), or 140% (high; HIH) of NRC requirements], initiated at d 40 of gestation. Ewes were fed individually and remained on treatments through parturition. All lambs were removed from their dams at birth and fed milk replacer. At 20.6 +/- 0.9 d of age, lambs were necropsied, visceral organs dissected, and jejunal samples collected. Lambs born to ewes fed CON and HIH had greater (P < 0.05) BW, gastrointestinal tract, stomach complex, and liver masses at necropsy than RES. Large intestinal and pancreatic masses, as well as stomach complex, large intestinal, and liver proportional masses, demonstrated (P <= 0.08) a nutritional plane x Se supply interaction. Proportional pancreatic mass was greater (P = 0.03) for lambs born to RES ewes than HIH. Although small intestinal mass was not affected (P >= 0.18) by gestational treatments, lambs born to HIH-fed ewes had greater (P <= 0.09) jejunal DNA concentration than RES and CON, and greater (P = 0.01) total DNA than RES. Nutritional plane and Se supply interacted to affect (P <= 0.003) jejunal percent proliferation and total proliferating small intestinal cells, although jejunal crypt depth and villus length were not affected by gestational treatment (P >= 0.17). Jejunal glucagon-like peptide-2 mRNA expression was greater (P <= 0.07) in lambs born to ewes fed RES compared with CON and HIH. Jejunal capillary size was affected (P = 0.09) by the interaction of nutritional plane x Se supply. Lambs from CON ewes had greater (P <= 0.04) jejunal capillary surface density than RES. Nutritional plane and Se supply interacted to affect (P = 0.07) jejunal soluble guanylate cyclase mRNA expression in a manner opposite of capillary size. In conclusion, neonatal lamb visceral organ mass was affected by gestational nutrition, even when lambs had ad libitum intake and similar management postnatally. Despite similar small intestinal mass at 20 d of age, jejunal growth, vascularity, and gene expression were altered by maternal nutrition during gestation. C1 [Meyer, A. M.; Neville, T. L.; Reed, J. J.; Reynolds, L. P.; Redmer, D. A.; Hammer, C. J.; Vonnahme, K. A.; Caton, J. S.] N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. [Taylor, J. B.] USDA ARS, US Sheep Expt Stn, Dubois, ID 83423 USA. RP Caton, JS (reprint author), N Dakota State Univ, Dept Anim Sci, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. EM joel.caton@ndsu.edu RI Reynolds, Lawrence/I-5267-2015 OI Reynolds, Lawrence/0000-0002-6838-7809 FU USDA-Cooperative State Research, Education, Extension Service National Research Initiative [2003-35206-13621, 2005-35206-15281]; National Institutes of Health [HL 64141] FX Partially supported by USDA-Cooperative State Research, Education, Extension Service National Research Initiative grants No. 2003-35206-13621 and 2005-35206-15281, and National Institutes of Health Grant HL 64141. Authors thank Pawel Borowicz, Roza Yunusova, Sharnae Klein, and Lara Woodworth for their assistance with image analysis and employees of the Animal Nutrition and Physiology Center and Ruminant Nutrition, Physiology, and Muscle Biology Laboratories at North Dakota State University for their contributions to this project. NR 49 TC 9 Z9 10 U1 3 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2628 EP 2639 DI 10.2527/jas.2012-5953 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200018 PM 23508034 ER PT J AU Cushman, RA Miles, JR Rempel, LA McDaneld, TG Kuehn, LA Chitko-McKown, CG Nonneman, D Echternkamp, SE AF Cushman, R. A. Miles, J. R. Rempel, L. A. McDaneld, T. G. Kuehn, L. A. Chitko-McKown, C. G. Nonneman, D. Echternkamp, S. E. TI Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE antral follicle count; cattle; reproductive longevity ID ANTRAL FOLLICLE COUNT; GENE-EXPRESSION; CORPUS-LUTEUM; OOCYTE QUALITY; MESSENGER-RNA; CUMULUS CELLS; CATTLE; WOMEN; DIFFERENTIATION; PERFORMANCE AB A proposed functional polymorphism in the ionotropic glutamate receptor AMPA1 (GRIA1) has been reported to influence antral follicle numbers and fertility in cows. Repeat breeder cows that fail to produce a calf in multiple seasons have been reported to have reduced numbers of small (1 to 3 mm) antral follicles in their ovaries. Therefore, we tested the hypothesis that this GRIA1 polymorphism was affecting antral follicle numbers in repeat breeder cows. Repeat breeder cows (n = 64) and control cows (n = 72) that had always produced a calf were housed in a dry lot and observed twice daily for behavioral estrus. Blood samples were collected, and cows were genotyped for this GRIA1 polymorphism and for a polymorphism in the GnRH receptor (GnRHR) that was proposed to influence age at puberty. On d 3 to 8 after estrus cows were slaughtered, and reproductive organs were collected to determine antral follicle count, ovary size, and uterine horn diameter. Repeat breeder cows were older at first calving than control cows (P = 0.006). The length (P = 0.03) and height (P = 0.02) of the ovary contralateral to the corpus luteum (CL) were greater in control cows than repeat breeder cows. The endometrial diameter in the horn ipsilateral to the CL was greater in the control cows than the repeat breeder cows. Repeat breeder cows had fewer small (1 to 5 mm) antral follicles than control cows (P = 0.003); however, there was no association between GRIA1 genotype and antral follicle number. The GnRHR polymorphism was associated with age at first calving because cows that were homozygous for the C allele had a greater age at first calving than heterozygous cows or cows that were homozygous for the T allele (P = 0.01). In the granulosa cells from small (1 to 5 mm) antral follicles, mRNA abundances of 2 markers of oocyte quality, anti-Mullerian hormone and pentraxin 3, did not differ between fertility groups (P >= 0.12). We conclude that this GRIA1 polymorphism exists in beef cows but that it does not influence antral follicle numbers. The association between GnRHR genotype and age at first calving is likely not causal as this polymorphism is not functional. The utility of this polymorphism as a genetic marker for early conception in heifers will require further validation. Screening postpartum cows by ultrasonography to determine antral follicle numbers may aid in making culling decisions. C1 [Cushman, R. A.; Miles, J. R.; Rempel, L. A.; McDaneld, T. G.; Kuehn, L. A.; Chitko-McKown, C. G.; Nonneman, D.; Echternkamp, S. E.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Cushman, RA (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM bob.cushman@ars.usda.gov FU ARS CRIS [5438-31000-093-00D] FX The authors gratefully acknowledge the assistance of Gordon Hays and the U. S. Meat Animal Research Center cattle crew for care and handling of the cows; Kevin Beavers, Jason Cushman, Suzy Hassler, Sue Hauver, Sandra Nejezchleb, Tammy Sorenson, and David Sypherd for expert technical assistance in the laboratory; and Linda Parnell for assistance with manuscript preparation. This research was funded by ARS CRIS number 5438-31000-093-00D entitled "Strategies to Improve Heifer Selection and Heifer Development" (R.A.C. and S.E.E.). NR 34 TC 8 Z9 8 U1 2 U2 9 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2640 EP 2646 DI 10.2527/jas.2012-5950 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200019 PM 23478821 ER PT J AU Song, R Chen, C Wang, L Johnston, LJ Kerr, BJ Weber, TE Shurson, GC AF Song, R. Chen, C. Wang, L. Johnston, L. J. Kerr, B. J. Weber, T. E. Shurson, G. C. TI High sulfur content in corn dried distillers grains with solubles protects against oxidized lipids by increasing sulfur-containing antioxidants in nursery pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE corn dried distillers grains with solubles; lipid peroxidation; nursery pigs; sulfur-containing antioxidants; vitamin E ID GROWER-FINISHER PIGS; AMINO-ACIDS; PERFORMANCE; DIETS; OXIDATION; TAURINE; GLUTATHIONE; METHIONINE; SULFATE; SWINE AB Some sources of corn dried distillers grains with solubles (DDGS) contain relatively high amounts of oxidized lipids produced from PUFA peroxidation during the production process. These oxidized lipids may impair metabolic oxidation status of pigs. The objective of this study was to understand the effects of feeding corn-soybean meal diets (CON) or diets containing 30% highly oxidized DDGS with 1 of 3 levels of supplemental vitamin E (dl-alpha-tocopheryl acetate), none, the 1998 NRC level (11 IU/kg), and 10x the 1998 NRC level (110 IU/kg), on oxidative status of nursery pigs. The DDGS source used in this study contained the greatest thiobarbituric acid reactive substances (TBARS) value, peroxide value, and total S content (5.2 ng/mg oil, 84.1 mEq/kg oil, and 0.95%, respectively) relative to 30 other DDGS sources sampled (mean values = 1.8 ng/mg oil, 11.5 mEq/kg oil, and 0.50%, respectively). Barrows (n = 54) were housed in pens and fed the experimental diets for 8 wk after weaning and transferred to individual metabolism cages for collection of feces, urine, blood, and liver samples. Total S content was greater in DDGS diets than in CON (0.39 vs. 0.19%). Dietary inclusion of 30% DDGS improved apparent total tract digestibility of S (86.8 vs. 84.6%; P < 0.001) and S retained (2.94 vs. 2.07 g/d; P < 0.01) compared with CON. Although pigs were fed highly oxidized DDGS in this study, serum TBARS were similar between DDGS and CON treatments. There was an interaction between DDGS and dietary vitamin E level for serum concentrations of alpha-tocopherol. Serum alpha-tocopherol concentrations were greater (P < 0.001) in pigs fed DDGS diets than those fed CON when dl-alpha-tocopheryl acetate was not provided or provided at the NRC level but were similar when dl-alpha-tocopheryl acetate was supplemented at the 10x NRC level. Pigs fed DDGS diets had greater serum concentrations of S-containing AA, particularly Met (P < 0.001) and taurine (P = 0.002), compared with those fed CON. Liver glutathione concentration was greater in pigs fed DDGS diets than CON (56.3 vs. 41.8 nmol/g). Dietary inclusion of DDGS (P < 0.001) and vitamin E (P = 0.03) increased enzyme activity of glutathione peroxidase. The elevated concentrations of S-containing antioxidants (Met, taurine, and glutathione) in vivo may protect pigs against oxidative stress when feeding highly oxidized DDGS. Therefore, the increased S content in DDGS may be beneficial, and increasing concentrations of vitamin E in diets may not be necessary to protect pigs against metabolic oxidative stress when feeding high S and highly peroxidized DDGS. C1 [Song, R.; Chen, C.; Shurson, G. C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. [Wang, L.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN 55108 USA. [Johnston, L. J.] Univ Minnesota, West Cent Res & Outreach Ctr, Morris, MN 56267 USA. [Kerr, B. J.; Weber, T. E.] ARS, USDA, NLAE, Ames, IA 50011 USA. RP Shurson, GC (reprint author), Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. EM shurs001@umn.edu FU National Pork Board (Des Moines, IA) FX Financial support was provided by the National Pork Board (Des Moines, IA). NR 54 TC 18 Z9 20 U1 2 U2 22 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2715 EP 2728 DI 10.2527/jas.2012-5350 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200026 PM 23482577 ER PT J AU Mulliniks, JT Kemp, ME Endecott, RL Cox, SH Roberts, AJ Waterman, RC Geary, TW Scholljegerdes, EJ Petersen, MK AF Mulliniks, J. T. Kemp, M. E. Endecott, R. L. Cox, S. H. Roberts, A. J. Waterman, R. C. Geary, T. W. Scholljegerdes, E. J. Petersen, M. K. TI Does beta-hydroxybutyrate concentration influence conception date in young postpartum range beef cows? SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cows; beta-hydroxybutyrate; conception date ID NONESTERIFIED FATTY-ACIDS; NEGATIVE-ENERGY BALANCE; DAIRY-COWS; REPRODUCTIVE-PERFORMANCE; SUBCLINICAL KETOSIS; BINDING-PROTEINS; EARLY LACTATION; BODY CONDITION; INSULIN; CATTLE AB Cows in negative energy balance after calving often have reduced reproductive performance, which is mediated by metabolic signals. The objective of these studies was to determine the association of serum metabolites, days to first postpartum ovulation, milk production, cow BW change, BCS, and calf performance with conception date in spring-calving 2- and 3-yr-old beef cows grazing native range. In Exp. 1, cows were classified by conception date in a 60-d breeding season as early (EARLY; conceived in first 15 d of breeding) or late conception (LATE; conceived during the last 45 d of breeding). Beginning on d 35 postpartum, blood samples were collected twice per week for serum metabolite analysis and progesterone analysis to estimate days to resumption of estrous cycles. As a chute-side measure of nutrient status and glucose sufficiency, whole-blood beta-hydroxybutyrate (BHB) concentrations were measured 14 +/- 2 d before breeding. In Exp. 2, cows were classified by subsequent calving date resulting from a 55 +/- 2 d breeding season as conceiving either early (EARLY; conceived in first 15 d of breeding) or late (LATE; conceived during the remaining breeding season). Blood samples were collected in 2 periods, 30 +/- 4 d before calving and 14 +/- 3 d before the initiation of breeding, to determine circulating concentrations of IGF-I and BHB. In Exp. 1, BHB and serum glucose concentrations were less (P <= 0.04) in EARLY cows than LATE cows. Serum insulin concentrations were greater (P = 0.03) in EARLY cows relative to LATE cows. Milk production and composition did not differ (P >= 0.24) by conception date groups. In Exp. 2, cow age x sample period x conception date interaction (P < 0.01) occurred for serum BHB concentrations. Serum BHB concentrations were similar (P > 0.10) for 2-yr-old cows (in greater nutritional plane compared with Exp. 1) regardless of their conception date classification and sampling period. However, precalving serum BHB concentrations were greater (P < 0.01) for LATE than EARLY in 3-yr-old cows with no difference (P = 0.86) at prebreeding. Serum IGF-1 concentrations were greater (P < 0.01) for EARLY cows relative to LATE cows at precalving and prebreeding. This study indicates that blood BHB concentrations during times of metabolic dysfunctions may provide a more sensitive indicator of energy status than body condition, predicting rebreeding competence in young beef cows as measured by interval from calving to conception. C1 [Mulliniks, J. T.; Kemp, M. E.; Cox, S. H.; Scholljegerdes, E. J.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. [Endecott, R. L.] Montana State Univ, Dept Anim & Range Sci, Miles City, MT 59301 USA. [Roberts, A. J.; Waterman, R. C.; Geary, T. W.; Petersen, M. K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Petersen, MK (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM mark.petersen@ars.usda.gov NR 34 TC 5 Z9 5 U1 0 U2 19 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2902 EP 2909 DI 10.2527/jas.2012-6029 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200046 PM 23478827 ER PT J AU Mirando, MA Swanson, KS AF Mirando, M. A. Swanson, K. S. TI COMPANION ANIMALS SYMPOSIUM: Nutrition special needs- the relationship between novel ingredients, environment, and gene expression SO JOURNAL OF ANIMAL SCIENCE LA English DT Editorial Material C1 [Mirando, M. A.] USDA, Inst Food Prod & Sustainabil, Div Anim Syst, Natl Inst Food & Agr, Washington, DC 20250 USA. [Swanson, K. S.] Univ Illinois, Div Nutr Sci, Urbana, IL 61801 USA. [Swanson, K. S.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Swanson, K. S.] Univ Illinois, Dept Vet Clin Med, Urbana, IL 61801 USA. RP Mirando, MA (reprint author), USDA, Inst Food Prod & Sustainabil, Div Anim Syst, Natl Inst Food & Agr, Washington, DC 20250 USA. EM mmirando@nifa.usda.gov NR 5 TC 0 Z9 0 U1 1 U2 9 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUN PY 2013 VL 91 IS 6 BP 2947 EP 2948 DI 10.2527/jas.2013-6634 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA 154UK UT WOS:000319701200051 PM 23723189 ER PT J AU Srinivasan, R Lumpkins, B Kim, E Fuller, L Jordan, J AF Srinivasan, Radhakrishnan Lumpkins, Brett Kim, Elizabeth Fuller, Lorraine Jordan, Joe TI Effect of fiber removal from ground corn, distillers dried grains with solubles, and soybean meal using the Elusieve process on broiler performance and processing yield SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE fiber removal; Elusieve; broiler; nutrition ID METABOLIZABLE ENERGY; AIR CLASSIFICATION; NUTRITIONAL-VALUE; SEPARATION; DIETS; FLOUR; DDGS AB The Elusieve process, a combination of sieving and elutriation (air classification), has been found to be effective in separating fiber from ground corn, distillers dried grains with solubles (DDGS), and soybean meal (SBM). The objective of this study was to determine the effect of removing fiber from ground corn, DDGS, and SBM on broiler live performance during the 42-d experimental period and assess the economic effect. A total of 6 dietary treatments were evaluated in which 3 treatments incorporated an additional nonstarch polysaccharide (NSP) enzyme corresponding to the following 3 treatments: regular diet, direct substituted enhanced diet, and an isocaloric, isonitrogenous enhanced diet. The study consisted of 48 pens with 45 male broiler chicks per pen. Elusieve processing increased starch content of corn by 7.8% and increased protein content of DDGS and SBM by 2.3 and 0.9%, respectively. Enhanced diets resulted in birds with 4.6 to 5.0% higher BW gain, higher breast weight by 7.1 to 11.3% and feed conversion improvement by 4 to 6 percentage points (2.4 to 3.2%) compared with regular diet. There was no effect of NSP enzyme on performance and feed consumption. Interaction effect (between NSP enzyme and dietary type) was observed only in 2 of a total of 12 performance indicators. The increase in profit due to implementation of the Elusieve process in a 1,000 t/d feed mill is estimated to be $0.5 to 2.5 million/yr, which is 0.8 to 4.3 cents/bird produced. The payback period is estimated to be 0.9 to 4.7 yr. C1 [Srinivasan, Radhakrishnan] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Lumpkins, Brett] Southern Poultry Res Inc, Athens, GA 30607 USA. [Kim, Elizabeth] USDA, ARS, Mississippi State, MS 39762 USA. [Fuller, Lorraine] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. [Jordan, Joe] Mississippi State Univ, Off Entrepreneurship & Technol Transfer, Mississippi State, MS 39762 USA. RP Srinivasan, R (reprint author), Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. EM rs634@msstate.edu NR 18 TC 0 Z9 0 U1 2 U2 22 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2013 VL 22 IS 2 BP 177 EP 189 DI 10.3382/japr.2012-00544 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA 152EP UT WOS:000319513900004 ER PT J AU Schmidt, AM Davis, JD Purswell, JL Fan, Z Kiess, AS AF Schmidt, A. M. Davis, J. D. Purswell, J. L. Fan, Z. Kiess, A. S. TI Spatial variability of heating profiles in windrowed poultry litter SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler litter; composting; kriging; windrow AB In-house windrow composting of broiler litter has been suggested as a means to reduce microbial populations between flocks. Published time-temperature goals are used to determine the success of the composting process for microbial reductions. Spatial and temporal density of temperature measurement can influence the accuracy in determining what portion of a windrow section has achieved specified time-temperature goals. In this study, windrow section temperature was recorded every 2 min for 7 d on a 10 x 10-cm grid in 183 (width) x 91 cm (height) windrow sections. In 5 windrow sections, ordinary kriging was used to predict the mean portion of the windrow cross-sectional area reaching time-temperature goals of 40 degrees C for 120 h, 50 degrees C for 24 h, and 55 degrees C for 4 h. Based on these results, 88.5 +/- 2.0%, 80.8 +/- 3.9%, and 38.4 +/- 11.7% of the windrow cross-sectional area can be expected to reach published microbial reduction time-temperature goals of 40 degrees C for 120 h, 50 degrees C for 24 h, and 55 degrees C for 4 h, respectively. This study illustrates the need to monitor temperature at multiple locations within windrowed litter to characterize heating profiles. Temporal and spatial sampling densities must be standardized to properly characterize temperature profiles in windrowed broiler litter. Additional research should be conducted to determine the degree of pathogen destruction achieved in the various time-temperature regions of the windrow pile. This study was useful in illustrating the efficacy (proportion of windrow cross-section) of windrow composting as a treatment method for reducing microbial populations as measured by time-temperature goals in used broiler litter. C1 [Schmidt, A. M.] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. [Davis, J. D.] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Purswell, J. L.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. [Fan, Z.] Mississippi State Univ, Dept Forestry, Forest & Water Resources Ctr, Mississippi State, MS 39762 USA. [Kiess, A. S.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. RP Davis, JD (reprint author), Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. EM jdavis@abe.msstate.edu NR 10 TC 0 Z9 0 U1 2 U2 8 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2013 VL 22 IS 2 BP 319 EP 328 DI 10.3382/japr.2012-00700 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 152EP UT WOS:000319513900020 ER PT J AU Stong, RA Kolodny, E Kelsey, RG Gonzalez-Hernandez, MP Vivanco, JM Manter, DK AF Stong, Rachel A. Kolodny, Eli Kelsey, Rick G. Gonzalez-Hernandez, M. P. Vivanco, Jorge M. Manter, Daniel K. TI Effect of Plant Sterols and Tannins on Phytophthora ramorum Growth and Sporulation SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Phytophthora ramorum; Tannin; Sterol; Elicitin; Protein Binding ID SEXUAL REPRODUCTION; CACTORUM; ELICITINS; CHOLESTEROL; TOBACCO; HOST; SPECIFICITY; INFESTANS; PROTEINS; PATHOGEN AB Elicitin-mediated acquisition of plant sterols is required for growth and sporulation of Phytophthora spp. This study examined the interactions between elicitins, sterols, and tannins. Ground leaf tissue, sterols, and tannin-enriched extracts were obtained from three different plant species (California bay laurel, California black oak, and Oregon white oak) in order to evaluate the effect of differing sterol/tannin contents on Phytophthora ramorum growth. For all three species, high levels of foliage inhibited P. ramorum growth and sporulation, with a steeper concentration dependence for the two oak samples. Phytophthora ramorum growth and sporulation were inhibited by either phytosterols or tannin-enriched extracts. High levels of sterols diminished elicitin gene expression in P. ramorum; whereas the tannin-enriched extract decreased the amount of 'functional' or ELISA-detectable elicitin, but not gene expression. Across all treatment combinations, P. ramorum growth and sporulation correlated strongly with the amount of ELISA-detectable elicitin (R (2) = 0.791 and 0.961, respectively). C1 [Stong, Rachel A.; Kolodny, Eli; Manter, Daniel K.] USDA ARS, Ft Collins, CO 80522 USA. [Kelsey, Rick G.] USDA Forest Serv, PNW Res Stn, Corvallis, OR USA. [Gonzalez-Hernandez, M. P.] Univ Santiago de Compostela, Dept Crop Prod, Lugo, Spain. [Vivanco, Jorge M.] Colorado State Univ, Dept Hort & Landscape Architecture, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. RP Manter, DK (reprint author), USDA ARS, Ft Collins, CO 80522 USA. EM daniel.manter@ars.usda.gov OI Gonzalez-Hernandez, M.P./0000-0002-0519-1702 FU US Department of Agriculture, Forest Service, Pacific Southwest Research Station; Ministry of Education and Science of Spain (Direccion General de Universidades) FX Funding for this research was provided by the US Department of Agriculture, Forest Service, Pacific Southwest Research Station. We thank Ellen Goheen, U.S. Forest Service, for assistance in collecting the black oak leaves in 2007, and Mr. Dale Gray for allowing the sampling of leaves from his ornamental California bay laurel in 2007 and 2008. We also thank Doug Westlind, U.S. Forest Service, for help collecting the black oak leaves in 2008. The research collaboration of M.P. Gonzalez-Hernandez was funded by the Ministry of Education and Science of Spain (Direccion General de Universidades). NR 45 TC 3 Z9 3 U1 0 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2013 VL 39 IS 6 BP 733 EP 743 DI 10.1007/s10886-013-0295-y PG 11 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 155TG UT WOS:000319770500004 PM 23689874 ER PT J AU Cheruiyot, DJ Boyd, RS Coudron, TA Cobine, PA AF Cheruiyot, Dorothy J. Boyd, Robert S. Coudron, Thomas A. Cobine, Paul A. TI Biotransfer, Bioaccumulation and Effects of Herbivore Dietary Co, Cu, Ni, and Zn on Growth and Development of the Insect Predator Podisus maculiventris (Say) SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Biotransfer; Bioaccumulation; Heavy metals; Herbivory; Info-disruption; Trophic effect; Hemiptera; Pentatomidae; Podisus maculiventris; Spodoptera exigua ID SPODOPTERA-EXIGUA LEPIDOPTERA; METAL HYPERACCUMULATION; HELIX-ASPERSA; PLANTS; NICKEL; SELENIUM; NOCTUIDAE; PHYTOREMEDIATION; RESPONSES; CADMIUM AB Increased metal availability in the environment can be detrimental for the growth and development of all organisms in a food web. In part, this toxicity is due to biotransfer or bioaccumulation of metals between trophic levels. We evaluated the survival, growth, and development of a generalist Hemipteran predator (Podisus maculiventris) when fed herbivorous prey (Spodoptera exigua) reared on artificial diet amended with Cu, Zn, Ni, and Co. Predator nymphs were fed S. exigua larvae raised on diet amended with sublethal (Minimum Sublethal Concentration or MSC) or lethal (Minimum Lethal Concentration or MLC) concentrations of each metal, as well as control diet. We determined if metals were biotransferred or bioaccumulated from the diet to herbivore and predator, as well as if predator growth or survival was affected by herbivore diet. Podisus maculiventris fed herbivores raised on MLC levels of both Cu and Zn took significantly longer to mature to adults, whereas their overall survival was not affected by prey diet metal concentration for any metal. Adult weights were significantly reduced for predators raised on herbivores reared on diets amended with the MLC of Cu and Zn. Copper and Zn were bioaccumulated from diet to herbivore and from herbivore to predator, whereas Ni was biotransferred (although concentrations decreased as trophic level increased). The pattern for Co was more complex, with biotransfer the main outcome. Our results show that availability of metals in a food web can affect growth and development of a hemipteran predator, and that metals are transferred between trophic levels, with metal-specific biotransfer and bioaccumulation outcomes. C1 [Cheruiyot, Dorothy J.] Brookstone Sch, Columbus, GA USA. [Boyd, Robert S.; Cobine, Paul A.] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. [Coudron, Thomas A.] Agr Res Serv, USDA, Columbia, MO USA. RP Boyd, RS (reprint author), Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. EM boydrob@auburn.edu RI Cobine, Paul/G-3107-2011 OI Cobine, Paul/0000-0001-6012-0985 NR 45 TC 3 Z9 4 U1 4 U2 63 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2013 VL 39 IS 6 BP 764 EP 772 DI 10.1007/s10886-013-0289-9 PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 155TG UT WOS:000319770500007 PM 23709043 ER PT J AU Moulton, LL Linz, GM Bleier, WJ AF Moulton, Laurel L. Linz, George M. Bleier, William J. TI Responses of territorial and floater male Red-winged Blackbirds to models of receptive females SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE after-second-year; Agelaius phoeniceus; extra-pair copulation; polygynous mating system; reproductive behavior; second-year ID EXTRA-PAIR PATERNITY; REPRODUCTIVE SUCCESS; DECEPTIVE ACQUISITION; TACHYCINETA-BICOLOR; TROPICAL PASSERINE; TREE SWALLOWS; BEHAVIOR; DENSITY; BIRDS; COPULATION AB Red-winged Blackbirds (RWBL; Agelaius phoeniceus) have a polygynous mating system and, because territorial males commonly have harems of two to five females, some second-year (SY) and after-second-year (ASY) males do not establish nesting territories, but become floaters. Previous studies have revealed high rates of extra-pair copulations in this species and that sexually mature male floaters and territory owners do not differ in size, testosterone levels, or reproductive capability, suggesting that floaters may occasionally gain paternity. During May and June 2008, we observed the behavioral responses of floater males to taxidermic mounts (models) of female RWBL placed in a precopulatory position. Floaters intruded into territories during 46% of model presentations, with 20% of intrusions by ASY floaters and 80% by SY floaters. During intrusions, ASY floaters attempted to copulate with models 93% of the time compared to 80% for SY floaters. Copulations were successful during 30% of attempts by ASY males and 25% of attempts by SY floaters. The frequency of intrusions by ASY and SY floaters, attempted copulations by SY floaters, and successful copulations by ASY floaters increased as territorial males spent more time off their territories. Responses of floater males toward models in our study suggest that floater male RWBL attempt to exploit available breeding opportunities. The lack of evidence for extrapair young (EPY) fathered by floater male RWBL in previous studies, combined with our results indicating that the presence of territorial males limits floater intrusions, copulation attempts, and successful copulations, suggests that the reproductive success of floater males is limited in part by the aggressive behavior of territorial males. C1 [Moulton, Laurel L.; Bleier, William J.] N Dakota State Univ, Fargo, ND 58105 USA. [Linz, George M.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. RP Moulton, LL (reprint author), N Dakota State Univ, 218 Stevens Hall, Fargo, ND 58105 USA. EM ummoulto@cc.umanitoba.ca FU U.S. Department of Agriculture (USDA) Wildlife Service's National Wildlife Research Center; Department of Biological Sciences, North Dakota State University FX We thank K. Yasukawa, S. Lupold, and K. Turner for reviewing an earlier draft of this manuscript. We also appreciate the advice and insight provided by the editor and three anonymous reviewers. We thank D. Westneat for his advice on creating models, L. Stokes and C. Doetkett for their statistical expertise, and J. Cochran for assisting with bird capture and data collection. The U.S. Geological Survey Bird Banding Lab granted a marking and salvage permit. The U.S. Fish and Wildlife Service and the North Dakota Game and Fish Department granted scientific collecting permits. Funding was provided by the U.S. Department of Agriculture (USDA) Wildlife Service's National Wildlife Research Center and the Department of Biological Sciences, North Dakota State University. Safety guidelines and animal care were reviewed and approved by the National Wildlife Research Center and North Dakota State University Institutional Animal Care and Use Committees. NR 55 TC 4 Z9 4 U1 0 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD JUN PY 2013 VL 84 IS 2 BP 160 EP 170 DI 10.1111/jofo.12014 PG 11 WC Ornithology SC Zoology GA 151QU UT WOS:000319476100004 ER PT J AU Byers, BE Kramer, BA Akresh, ME King, DI AF Byers, Bruce E. Kramer, Brodie A. Akresh, Michael E. King, David I. TI Interspecific song imitation by a Prairie Warbler SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE birdsong; Field Sparrow; Setophaga discolor; Spizella pusilla; vocal learning ID FIELD SPARROW; SIGNALS AB Song development in oscine songbirds relies on imitation of adult singers and thus leaves developing birds vulnerable to potentially costly errors caused by imitation of inappropriate models, such as the songs of other species. In May and June 2012, we recorded the songs of a bird that made such an error: a male Prairie Warbler (Setophaga discolor) in western Massachusetts that sang songs seemingly acquired by imitating the songs of a Field Sparrow (Spizella pusilla). Another song type in the bird's repertoire was a near-normal Group A Prairie Warbler song, but the bird used this song in contexts normally reserved for Group B songs. Despite its abnormal singing behavior, the aberrant bird successfully defended a territory and attracted a mate that laid two clutches of eggs. Results of playbacks of the focal bird's heterospecific song suggested that neighboring conspecific males learned to associate the Field Sparrow-like song with the focal male, and responded to the song as if it were a Prairie Warbler song. Our evidence suggests that the focal bird's aberrant singing evoked normal responses from potential mates and rivals. If such responses are widespread among songbirds, the general failure of heterospecific songs, once acquired, to spread through populations by cultural transmission is probably not attributable to a lack of recognition by conspecifics of the songs of heterospecific singers. C1 [Byers, Bruce E.; Kramer, Brodie A.] Univ Massachusetts, Dept Biol, Morrill Sci Ctr 221, Amherst, MA 01003 USA. [Akresh, Michael E.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [King, David I.] Univ Massachusetts, US Forest Serv, No Res Stn, Amherst, MA 01003 USA. RP Byers, BE (reprint author), Univ Massachusetts, Dept Biol, Morrill Sci Ctr 221, Amherst, MA 01003 USA. EM bbyers@bio.umass.edu FU U.S. Forest Service, Northern Research Station FX We thank D. Nelson for identifying the Field Sparrow complex song as a potential model for our nonconformist singer. Dr. Nelson also provided helpful comments on an earlier draft of this paper, as did M. R. Lein and an anonymous reviewer. D. Weidemann, N. Young, A. Bielaski, and A. Keel provided invaluable assistance in the field. The observations reported here were gathered as part of a larger project funded by the U.S. Forest Service, Northern Research Station. NR 23 TC 1 Z9 1 U1 0 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD JUN PY 2013 VL 84 IS 2 BP 181 EP 186 DI 10.1111/jofo.12016 PG 6 WC Ornithology SC Zoology GA 151QU UT WOS:000319476100006 ER PT J AU Vukovich, M Kilgo, JC AF Vukovich, Mark Kilgo, John C. TI Fall movements of Red-headed Woodpeckers in South Carolina SO JOURNAL OF FIELD ORNITHOLOGY LA English DT Article DE facultative migration; fall migration; home range; Melanerpes erythrocephalus; short-distance migratory movements ID BEHAVIOR; BIRDS AB Fall migration of Red-headed Woodpeckers (Melanerpes erythrocephalus) can be erratic, with departure rates, directions, and distances varying among populations and individuals. We report fall migration departure dates, rates, and routes, and the size of fall home ranges of 62 radio-tagged Red-headed Woodpeckers in western South Carolina. Rates of fall migration differed among years; all radio-tagged woodpeckers migrated in 2005 (15 of 15), none (0 of 23) migrated in 2006, and 54.2% (13 of 24) migrated in 2007. Of 28 woodpeckers that left their breeding territories, we relocated eight either en route or on their fall home ranges. These woodpeckers migrated short distances (4.322.2 km) south along the floodplain forest of a large creek. The variable migration patterns we observed indicate that Red-headed Woodpeckers may best be described as facultative migrants. We determined the home range sizes of 13 woodpeckers in both seasons, regardless of whether they migrated, and fall home ranges were smaller (mean= 1.12 ha) than summer home ranges (mean= 3.23 ha). Fall-winter movements of Red-headed Woodpeckers were concentrated on mast-producing oak (Quercus spp.) trees, which may have restricted home range sizes. The partial migration we observed in 2007 suggests that factors other than mast crop variability may also influence migration patterns because woodpeckers that year responded to the same annual mast crop in different ways, with some migrating and some remaining on breeding season home ranges during the fall. C1 [Vukovich, Mark; Kilgo, John C.] US Forest Serv, USDA, Southern Res Stn, New Ellenton, SC 29809 USA. RP Vukovich, M (reprint author), US Forest Serv, USDA, Southern Res Stn, POB 700, New Ellenton, SC 29809 USA. EM mvukovich@fs.fed.us FU U.S. Department of Energy-Savannah River Operations Office through the USDA Forest Service Savannah River [DE-AI09-00SR22188]; USDA Forest Service Southern Research Station FX We thank K. Legleu, K. Nayda, and K. Frier for assistance in the field, J. Blake and K. Wright for logistical support, S. Zarnoch for statistical assistance, and D. Ingold and S. Lohr for reviewing the manuscript. Funding for our research was provided by the U.S. Department of Energy-Savannah River Operations Office through the USDA Forest Service Savannah River under Interagency Agreement No. DE-AI09-00SR22188, and by the USDA Forest Service Southern Research Station. NR 27 TC 1 Z9 1 U1 1 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0273-8570 J9 J FIELD ORNITHOL JI J. Field Ornithol. PD JUN PY 2013 VL 84 IS 2 BP 193 EP 200 DI 10.1111/jofo.12018 PG 8 WC Ornithology SC Zoology GA 151QU UT WOS:000319476100008 ER PT J AU Seilheimer, TS Zimmerman, PL Stueve, KM Perry, CH AF Seilheimer, Titus S. Zimmerman, Patrick L. Stueve, Kirk M. Perry, Charles H. TI Landscape-scale modeling of water quality in Lake Superior and Lake Michigan watersheds: How useful are forest-based indicators? SO JOURNAL OF GREAT LAKES RESEARCH LA English DT Article DE Forest disturbance; Water quality; Great Lakes; Landscape; Mixed effects model ID LAND-USE; GREAT-LAKES; STREAMS; PHOSPHORUS; TRANSPORT; PATTERNS; REGION; BASIN AB The Great Lakes watersheds have an important influence on the water quality of the nearshore environment, therefore, watershed characteristics can be used to predict what will be observed in the streams. We used novel landscape information describing the forest cover change, along with forest census data and established land cover data to predict total phosphorus and turbidity in Great Lakes streams. In Lake Superior, we modeled increased phosphorus as a function of the increase in the proportion of persisting forest, forest disturbed during 2000-2009, and agricultural land, and we modeled increased turbidity as a function of the increase in the proportion of persisting forest, forest disturbed during 2000-2009, agricultural land, and urban land. In Lake Michigan, we modeled increased phosphorus as a function of ecoregion, decrease in the proportion of forest disturbed during 1984-1999 and watershed storage, and increase in the proportion of urban land, and we modeled increased turbidity as a function of ecoregion, increase in the proportion of forest disturbed during 2000-2009, and decrease in the proportion softwood forest. We used these relationships to identify priority areas for restoration in the Lake Superior basin in the southwestern watersheds, and in west central and southwest watersheds of the Lake Michigan basin. We then used the models to estimate water quality in watersheds without observed instream data to prioritize those areas for management. Prioritizing watersheds will aid effective management of the Great Lakes watershed and result in efficient use of restoration funds, which will lead to improved nearshore water quality. Published by Elsevier B.V. on behalf of International Association for Great Lakes Research. C1 [Seilheimer, Titus S.; Zimmerman, Patrick L.; Stueve, Kirk M.; Perry, Charles H.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Zimmerman, Patrick L.] Univ Minnesota, Sch Stat, Minneapolis, MN 55455 USA. RP Seilheimer, TS (reprint author), UW Manitowoc, UW Sea Grant Inst, 705 Viebahn St, Manitowoc, WI 54220 USA. EM tseilheimer@aqua.wisc.edu; patzimm@umn.edu; kstueve@biogeography.us; charleshpeny@fs.fed.us OI Perry, Charles/0000-0001-9377-5996 FU U.S. Environmental Protection Agency through the Great Lakes Restoration Initiative FX Funding for this research was provided by the U.S. Environmental Protection Agency through the Great Lakes Restoration Initiative. The USEPA was not involved in the design, analysis, or writing of this manuscript Valuable comments on earlier drafts were provided by R. Kolka, B. Tavernia, J. Stanovick, E. Rutherford, and the anonymous reviewers. The mention of trade names does not imply an official endorsement of the product. NR 53 TC 4 Z9 4 U1 3 U2 48 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0380-1330 J9 J GREAT LAKES RES JI J. Gt. Lakes Res. PD JUN PY 2013 VL 39 IS 2 BP 211 EP 223 DI 10.1016/j.jglr.2013.03.012 PG 13 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 155YH UT WOS:000319785900003 ER PT J AU Schneider, M DeBar, L Calingo, A Hall, W Hindes, K Sleigh, A Thompson, D Volpe, SL Zeveloff, A Pham, T Steckler, A AF Schneider, Margaret DeBar, Lynn Calingo, Ashley Hall, Will Hindes, Katie Sleigh, Adriana Thompson, Debbe Volpe, Stella L. Zeveloff, Abby Trang Pham Steckler, Allan TI The Effect of a Communications Campaign on Middle School Students' Nutrition and Physical Activity: Results of the HEALTHY Study SO JOURNAL OF HEALTH COMMUNICATION LA English DT Article ID INTERVENTION; EPIDEMIC; CHILDREN; OBESITY; YOUTH AB The HEALTHY Study was a 3-year school-based intervention designed to change the behaviors of middle school students to reduce their risk for developing type 2 diabetes mellitus. This report examines the relation between exposure to communications campaign materials and behavior change among students in the HEALTHY intervention schools. Using data from campaign tracking logs and student interviews, the authors examined communications campaign implementation and exposure to the communications campaign as well as health behavior change. Campaign tracking documents revealed variability across schools in the quantity of communications materials disseminated. Student interviews confirmed that there was variability in the proportion of students who reported receiving information from the communication campaign elements. Correlations and regression analysis controlling for semester examined the association between campaign exposure and behavior change across schools. There was a significant association between the proportion of students exposed to the campaign and the proportion of students who made changes in health behavior commensurate with study goals. The results suggest that, in the context of a multifaceted school-based health promotion intervention, schools that achieve a higher rate of exposure to communication campaign materials among the students may stimulate greater health behavior change. C1 [Schneider, Margaret] Univ Calif Irvine, Irvine, CA 92697 USA. [DeBar, Lynn] Kaiser Permanente Ctr Hlth Res, Portland, OR USA. [Calingo, Ashley; Hall, Will; Zeveloff, Abby; Steckler, Allan] Univ N Carolina, Chapel Hill, NC USA. [Hindes, Katie] Univ Pittsburgh, Grad Sch Publ Hlth, Pittsburgh, PA USA. [Sleigh, Adriana] Oregon Hlth & Sci Univ, Portland, OR 97201 USA. [Thompson, Debbe] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Volpe, Stella L.] Univ Penn, Sch Nursing, Div Biobehav & Hlth Sci, Philadelphia, PA 19104 USA. [Trang Pham] George Washington Univ, Ctr Biostat, Rockville, MD USA. RP Schneider, M (reprint author), Univ Calif Irvine, SE258, Irvine, CA 92697 USA. EM margaret.schneider@uci.edu FU NIDDK NIH HHS [U01 DK061231, U01-DK61230, U01-DK61231, U01-DK61223, U01 DK061249, U01 DK061230, U01-DK61249, U01 DK061223] NR 18 TC 4 Z9 4 U1 0 U2 15 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1081-0730 J9 J HEALTH COMMUN JI J. Health Commun. PD JUN 1 PY 2013 VL 18 IS 6 BP 649 EP 667 DI 10.1080/10810730.2012.743627 PG 19 WC Communication; Information Science & Library Science SC Communication; Information Science & Library Science GA 159AL UT WOS:000320016300003 PM 23409792 ER PT J AU Zhao, AP Yang, ZZ Sun, R Grinchuk, V Netzel-Arnett, S Anglin, IE Driesbaugh, KH Notari, L Bohl, JA Madden, KB Urban, JF Antalis, TM Shea-Donohue, T AF Zhao, Aiping Yang, Zhonghan Sun, Rex Grinchuk, Viktoryia Netzel-Arnett, Sarah Anglin, Ian E. Driesbaugh, Kathryn Hodge Notari, Luigi Bohl, Jennifer A. Madden, Kathleen B. Urban, Joseph F., Jr. Antalis, Toni M. Shea-Donohue, Terez TI SerpinB2 Is Critical to Th2 Immunity against Enteric Nematode Infection SO JOURNAL OF IMMUNOLOGY LA English DT Article ID EPITHELIAL-CELL FUNCTION; ALTERNATIVELY ACTIVATED MACROPHAGES; INHIBITOR TYPE-2; KAPPA-B; RECEPTOR; IL-13; GENE; PROTECTION; APOPTOSIS; PARASITES AB SerpinB2, a member of the serine protease inhibitor family, is expressed by macrophages and is significantly upregulated by inflammation. Recent studies implicated a role for SerpinB2 in the control of Thl and Th2 immune responses, but the mechanisms of these effects are unknown. In this study, we used mice deficient in SerpinB2 (SerpinB2(-/-)) to investigate its role in the host response to the enteric nematode, Heligmosomoides bakeri. Nematode infection induced a STAT6-dependent increase in intestinal SerpinB2 expression. The H. bakeri induced upregulation of IL-4 and IL-13 expression was attenuated in SerpinB2(-/-) mice coincident with an impaired worm clearance. In addition, lack of SerpinB2 in mice resulted in a loss of the H. bakeri induced smooth muscle hypercontractility and a significant delay in infection-induced increase in mucosal permeability. Th2 immunity is generally linked to a CCL2-mediated increase in the infiltration of macrophages that develop into the alternatively activated phenotype (M2). In H. bakeri infected SerpinB2(-/-) mice, there was an impaired infiltration and alternative activation of macrophages accompanied by a decrease in the intestinal CCL2 expression. Studies in macrophages isolated from SerpinB2(-/-) mice showed a reduced CCL2 expression, but normal M2 development, in response to stimulation of Th2 cytokines. These data demonstrate that the immune regulation of SerpinB2 expression plays a critical role in the development of Th2-mediated protective immunity against nematode infection by a mechanism involving CCL2 production and macrophage infiltration. C1 [Zhao, Aiping; Yang, Zhonghan; Sun, Rex; Grinchuk, Viktoryia; Notari, Luigi; Bohl, Jennifer A.; Shea-Donohue, Terez] Univ Maryland, Sch Med, Mucosal Biol Res Ctr, Dept Med, Baltimore, MD 21201 USA. [Netzel-Arnett, Sarah; Anglin, Ian E.; Driesbaugh, Kathryn Hodge; Antalis, Toni M.] Univ Maryland, Sch Med, Ctr Vasc & Inflammatory Dis, Baltimore, MD 21201 USA. [Madden, Kathleen B.] Uniformed Serv Univ Hlth Sci, Dept Pediat, Bethesda, MD 20814 USA. [Urban, Joseph F., Jr.] USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. RP Shea-Donohue, T (reprint author), Univ Maryland, Sch Med, Mucosal Biol Res Ctr, 20 Penn St, Baltimore, MD 21201 USA. EM tdonohue@mbrc.umaryland.edu OI Urban, Joseph/0000-0002-1590-8869 FU National Institutes of Health [R01-DK083418, R01-AI/DK49316, R01-DK081376, R56-CA098369]; U.S. Department of Agriculture [1235-51000-055]; Maryland Stem Cell Research Fund [0145-00] FX This work was supported by National Institutes of Health Grants R01-DK083418 (to AZ), R01-AI/DK49316 (to TS-D), R01-DK081376 (to T.M.A.), and R56-CA098369 (to T.M.A.); U.S. Department of Agriculture Current Research Information System Project 1235-51000-055 (to J.F.U.), and Maryland Stem Cell Research Fund Grant 0145-00 (to S.N.-A.). NR 34 TC 10 Z9 10 U1 0 U2 4 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JUN 1 PY 2013 VL 190 IS 11 BP 5779 EP 5787 DI 10.4049/jimmunol.1200293 PG 9 WC Immunology SC Immunology GA 147XY UT WOS:000319205900049 PM 23630350 ER PT J AU Bitsadze, N Jaronski, S Khasdan, V Abashidze, E Abashidze, M Latchininsky, A Samadashvili, D Sokhadze, I Rippa, M Ishaaya, I Horowitz, AR AF Bitsadze, N. Jaronski, S. Khasdan, V. Abashidze, E. Abashidze, M. Latchininsky, A. Samadashvili, D. Sokhadze, I. Rippa, M. Ishaaya, I. Horowitz, A. R. TI Joint action of Beauveria bassiana and the insect growth regulators diflubenzuron and novaluron, on the migratory locust, Locusta migratoria SO JOURNAL OF PEST SCIENCE LA English DT Article DE Entomopathogen; IGR; Beauveria; Diflubenzuron; Novaluron ID POTATO BEETLE COLEOPTERA; ENTOMOPATHOGENIC FUNGUS; DESERT LOCUST; METARHIZIUM-ANISOPLIAE; BALSAMO VUILLEMIN; ENZYME-ACTIVITY; COMPATIBILITY; CHRYSOMELIDAE; GRASSHOPPERS; SYNERGISM AB Beauveria bassiana (Balsamo) Vuillemin and sublethal concentrations of the insect growth regulators (IGR) diflubenzuron and novaluron were applied simultaneously and sequentially to second-instar Locusta migratoria migratorioides (Sauss.) to determine the interaction between these materials and an entomopathogenic fungus. Nymphs were fed on corn leaf disks treated with several concentrations of each IGR, and a constant dose of B. bassiana was applied topically. Additive interaction was demonstrated in all instances when second-instar nymphs were exposed to diflubenzuron or novaluron simultaneously with B. bassiana treatment, and when the fungus was applied first and IGR after 48 h. Additive interaction was also exhibited when novaluron was applied first and B. bassiana after 48 h. Antagonism was demonstrated when nymphs were fed diflubenzuron-treated corn leaves first, then after 48 h were treated with B. bassiana. The additive interaction that was observed in our experiments could still be useful to achieve efficacious levels of acridid control at low rates of an IGR along with a biological control agent such as an entomopathogenic fungus. C1 [Bitsadze, N.; Abashidze, E.; Samadashvili, D.] Kanchaveli L Inst Plant Protect, Dept Entomol & Appl Zool, Tbilisi, Rep of Georgia. [Jaronski, S.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT USA. [Khasdan, V.] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel. [Abashidze, E.] Kanchaveli L Inst Plant Protect, Dept Phytopathol, Tbilisi, Rep of Georgia. [Abashidze, M.] Natl Acad Sci, Tbilisi, Rep of Georgia. [Latchininsky, A.] Univ Wyoming, Dept Renewable Resources, Entomol Sect, Laramie, WY 82071 USA. [Sokhadze, I.] Javakhishvili State Univ, Tbilisi, Rep of Georgia. [Rippa, M.; Horowitz, A. R.] ARO, Gilat Res Ctr, Dept Entomol, Negev, Israel. [Ishaaya, I.] ARO, Volcani Ctr, Dept Entomol, Bet Dagan, Israel. RP Jaronski, S (reprint author), USDA ARS, Northern Plains Agr Res Lab, Sidney, MT USA. EM stefan.jaronski@ars.usda.gov FU US Israel Cooperative Development Research Program, Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development [CA23-022] FX We thank Galina Gindin and Marina Hazanovski for advice and all workers of Entomology Department of Gilat Research Center for their help. This research was supported (in part) under Grant No. CA23-022 funded by the US Israel Cooperative Development Research Program, Bureau for Economic Growth, Agriculture, and Trade, US Agency for International Development. NR 42 TC 5 Z9 5 U1 3 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 J9 J PEST SCI JI J. Pest Sci. PD JUN PY 2013 VL 86 IS 2 BP 293 EP 300 DI 10.1007/s10340-012-0476-4 PG 8 WC Entomology SC Entomology GA 147KQ UT WOS:000319166100018 ER PT J AU Senay, GB Bohms, S Singh, RK Gowda, PH Velpuri, NM Alemu, H Verdin, JP AF Senay, Gabriel B. Bohms, Stefanie Singh, Ramesh K. Gowda, Prasanna H. Velpuri, Naga M. Alemu, Henok Verdin, James P. TI Operational Evapotranspiration Mapping Using Remote Sensing and Weather Datasets: A New Parameterization for the SSEB Approach SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE drought; evapotranspiration; irrigation; remote sensing; water use ID SURFACE-ENERGY BALANCE; TEMPERATURE; EVAPORATION; MODEL; SCALE; ALGORITHM; FLUXES AB The increasing availability of multi-scale remotely sensed data and global weather datasets is allowing the estimation of evapotranspiration (ET) at multiple scales. We present a simple but robust method that uses remotely sensed thermal data and model-assimilated weather fields to produce ET for the contiguous United States (CONUS) at monthly and seasonal time scales. The method is based on the Simplified Surface Energy Balance (SSEB) model, which is now parameterized for operational applications, renamed as SSEBop. The innovative aspect of the SSEBop is that it uses predefined boundary conditions that are unique to each pixel for the hot and cold reference conditions. The SSEBop model was used for computing ET for 12years (2000-2011) using the MODIS and Global Data Assimilation System (GDAS) data streams. SSEBop ET results compared reasonably well with monthly eddy covariance ET data explaining 64% of the observed variability across diverse ecosystems in the CONUS during 2005. Twelve annual ET anomalies (2000-2011) depicted the spatial extent and severity of the commonly known drought years in the CONUS. More research is required to improve the representation of the predefined boundary conditions in complex terrain at small spatial scales. SSEBop model was found to be a promising approach to conduct water use studies in the CONUS, with a similar opportunity in other parts of the world. The approach can also be applied with other thermal sensors such as Landsat. C1 [Senay, Gabriel B.] USGS Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. [Bohms, Stefanie] SGT Inc, Sioux Falls, SD 57198 USA. [Singh, Ramesh K.; Velpuri, Naga M.] ARTS, Sioux Falls, SD 57198 USA. [Gowda, Prasanna H.] USDA ARS, Bushland, TX 79012 USA. [Alemu, Henok] S Dakota State Univ, GISc Ctr Excellence, Brookings, SD 57007 USA. [Verdin, James P.] USGS EROS Ctr, Boulder, CO 80305 USA. RP Senay, GB (reprint author), USGS Earth Resources Observat & Sci EROS Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. EM senay@usgs.gov RI Velpuri, Naga Manohar/A-5193-2013; OI Velpuri, Naga Manohar/0000-0002-6370-1926; Singh, Ramesh/0000-0002-8164-3483 FU U.S. Geological Survey (USGS) [G08PC91508, G10PC00044] FX This work was performed under U.S. Geological Survey (USGS) contract G08PC91508 and G10PC00044 in support of the Water-SMART project. The authors are thankful to the journal editor and three anonymous reviewers for their constructive and helpful suggestions to improve the manuscript. They gratefully acknowledge the use of AmeriFlux data for their model validation. The use of trade, firm, or corporation names in this article is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Geological Survey or the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 24 TC 35 Z9 35 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD JUN PY 2013 VL 49 IS 3 BP 577 EP 591 DI 10.1111/jawr.12057 PG 15 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 157LL UT WOS:000319897500008 ER PT J AU Klun, JA Kramer, M Debboun, M AF Klun, Jerome A. Kramer, Matthew Debboun, Mustapha TI Four simple stimuli that induce host-seeking and blood-feeding behaviors in two mosquito species, with a clue to DEET's mode of action SO JOURNAL OF VECTOR ECOLOGY LA English DT Article DE Mosquito repellent; mosquito wind tunnel; ATP; water vapor; carbon dioxide ID HUMAN SKIN EMANATIONS; AEDES-AEGYPTI; CARBON-DIOXIDE; GORGING RESPONSE; BIOASSAY SYSTEM; ATP ANALOGS; WIND-TUNNEL; CULICIDAE; DIPTERA; IDENTIFICATION AB Bioassays in a wind tunnel showed that a combination of four stimuli releases intense host-seeking and blood-feeding behavioral responses from females of the Asian tiger mosquito, Aedes albopictus, and the yellow fever mosquito, Aedes aegypti. The stimuli are carbon dioxide, water vapor, warmth, and adenosine triphosphate (ATP). Mosquitoes responded to this combination with a repertoire of blood-feeding behaviors that included upwind flight, landing, probing, and engorgement. Absence of carbon dioxide, water vapor, or ATP from the combination of stimuli or exposure to temperatures 12 degrees C below or above human-host temperature (38 degrees C) significantly attenuated blood-feeding behavior in both species. Although there is literature documenting the individual importance of each of these stimuli, our work represents the first instance where this combination of stimuli was found sufficient to elicit a complete repertoire of blood-feeding behaviors in these mosquitoes without involvement of any host specific odor. When mosquitoes were exposed to the four stimuli along with N,N-diethyl-3-methylbenzamide (DEET), feeding behavior was greatly suppressed. We hypothesize that a possible mode of action for DEET against these mosquitoes involves interference of warmth and/or water vapor receptors. An electrophysiological study designed to determine if DEET adversely affects the function of these receptors would be illuminating. C1 [Klun, Jerome A.] ARS, USDA, Potomac, MD 20854 USA. [Kramer, Matthew] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Debboun, Mustapha] USA, Med Dept Ctr & Sch, Acad Hlth Sci, Dept Prevent Hlth Serv, Ft Sam Houston, TX 78234 USA. RP Kramer, M (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM matt.kramer@ars.usda.gov NR 40 TC 7 Z9 7 U1 3 U2 20 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 J9 J VECTOR ECOL JI J. Vector Ecol. PD JUN PY 2013 VL 38 IS 1 BP 143 EP 153 DI 10.1111/j.1948-7134.2013.12020.x PG 11 WC Entomology SC Entomology GA 150VX UT WOS:000319420600019 PM 23701619 ER PT J AU Nalen, CMZ Allan, SA Becnel, JJ Kaufman, PE AF Nalen, Catherine M. Zettel Allan, Sandra A. Becnel, James J. Kaufman, Phillip E. TI Oviposition substrate selection by Florida mosquitoes in response to pathogen-infected conspecific larvae SO JOURNAL OF VECTOR ECOLOGY LA English DT Article DE Aedes aegypti; Aedes albopictus; Culex quinquefasciatus; Edhazardia aedis; Vavraia culicis; CuniNPV; oviposition ID AEDES-AEGYPTI DIPTERA; PLAGIORCHIS-ELEGANS TREMATODA; CULEX-QUINQUEFASCIATUS; ALBOPICTUS DIPTERA; HABITAT SELECTION; VAVRAIA-CULICIS; EGG RAFTS; MICROSPORIDIA; NIGRIPALPUS; INFUSIONS AB The impact of the presence of larval mosquito pathogens with potential for biological control on oviposition choice was evaluated for three mosquito species/pathogen pairs present in Florida. These included Aedes aegypti infected with Edhazardia aedis, Aedes albopictus infected with Vavraia culicis, and Culex quinquefasciatus infected with Culex nigripalpus nucleopolyhedrovirus (CuniNPV). Two-choice oviposition bioassays were performed on each host and pathogen species with one oviposition cup containing infected larvae and the other cup containing uninfected larvae (control). Both uninfected and E. aedis-infected female Ae. aegypti laid significantly fewer eggs in oviposition cups containing infected larvae. Uninfected gravid female Ae. albopictus and Cx. quinquefasciatus oviposited equally in cups containing uninfected larvae or containing larvae infected with V. culicis or CuniNPV, respectively. Gravid female Ae. albopictus infected with V. culicis did not display ovarian development and did not lay eggs. The decreased oviposition by gravid Ae. aegypti in containers containing E. aedis-infected larvae may indicate that the infected larvae produce chemicals deterring oviposition. C1 [Nalen, Catherine M. Zettel; Allan, Sandra A.; Becnel, James J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Kaufman, Phillip E.] Univ Florida, Entomol & Nematol Dept, Gainesville, FL 32611 USA. RP Allan, SA (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM sandy.allan@ars.usda.gov OI Kaufman, Phillip/0000-0001-6159-8358 FU University of Florida Entomology and Nematology Department FX We thank Neil Sanscrainte, Erin Vrzal-Wessels, Haze Brown, and Julie McClurg for their technical support and guidance, and the University of Florida Entomology and Nematology Department for providing financial support through a graduate assistantship. NR 44 TC 1 Z9 2 U1 3 U2 50 PU SOC VECTOR ECOLOGY PI CORONA PA 1966 COMPTON AVE, CORONA, CA 92881 USA SN 1081-1710 J9 J VECTOR ECOL JI J. Vector Ecol. PD JUN PY 2013 VL 38 IS 1 BP 182 EP 187 DI 10.1111/j.1948-7134.2013.12025.x PG 6 WC Entomology SC Entomology GA 150VX UT WOS:000319420600024 ER PT J AU Kwak, YS Weller, DM AF Kwak, Youn-Sig Weller, David M. TI Take-all of Wheat and Natural Disease Suppression: A Review SO PLANT PATHOLOGY JOURNAL LA English DT Article DE 2,4-DAPG; biological control; Gaeumannomyces graminis var. tritici; Pseudomonas spp.; take-all decline ID 2,4-DIACETYLPHLOROGLUCINOL-PRODUCING PSEUDOMONAS-FLUORESCENS; INDUCED SYSTEMIC RESISTANCE; GRAMINIS VAR TRITICI; BLACK ROOT-ROT; BIOLOGICAL-CONTROL; GAEUMANNOMYCES-GRAMINIS; ANTIBIOTIC PRODUCTION; ARABIDOPSIS-THALIANA; GENOTYPIC DIVERSITY; RHIZOSPHERE COLONIZATION AB In agro-ecosystems worldwide, some of the most important and devastating diseases are caused by soil-borne necrotrophic fungal pathogens, against which, crop plants generally lack genetic resistance. However, plants have evolved approaches to protect themselves against pathogens by stimulating and supporting specific groups of beneficial microorganisms that have the ability to protect either by direct inhibition of the pathogen or by inducing resistance mechanisms in the plant. One of the best examples of protection of plant roots by antagonistic microbes occurs in soils that are suppressive to take-all disease of wheat. Take-all, caused by Gaeumannomyces graminis var. tritici, is the most economically important root disease of wheat worldwide. Take-all decline (TAD) is the spontaneous decline in incidence and severity of disease after a severe outbreak of take-all during continuous wheat or barley monoculture. TAD occurs worldwide, and in the United States and The Netherlands it results from a build-up of populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. during wheat monoculture. The antibiotic 2,4-DAPG has a broad spectrum of activity and is especially active against the take-all pathogen. Based on genotype analysis by repetitive sequence-based-PCR analysis and restriction fragment length polymorphism of phlD, a key 2,4-DAPG biosynthesis gene, at least 22 genotypes of 2,4-DAPG producing fluorescent Pseudomonas spp. have been described worldwide. In this review, we provide an overview of G graminis var. tritici, the take-all disease, Pseudomonas biocontrol agents, and mechanism of disease suppression. C1 [Kwak, Youn-Sig] Gyeongsang Natl Univ, Dept Appl Biol, Jinju 660701, South Korea. [Kwak, Youn-Sig] Gyeongsang Natl Univ, Inst Agr & Life Sci, Jinju 660701, South Korea. [Weller, David M.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. RP Kwak, YS (reprint author), Gyeongsang Natl Univ, Dept Appl Biol, Jinju 660701, South Korea. EM kwak@gnu.ac.kr NR 85 TC 9 Z9 11 U1 10 U2 103 PU KOREAN SOC PLANT PATHOLOGY PI SUWON PA NATIONAL INST AGRICULTURAL SCIENCE & TECHNOLOGY, PLANT PATHOLOGY DIVISION, SUWON, 441-707, SOUTH KOREA SN 1598-2254 J9 PLANT PATHOLOGY J JI Plant Pathol. J. PD JUN PY 2013 VL 29 IS 2 SI SI BP 125 EP 135 DI 10.5423/PPJ.SI.07.2012.0112 PG 11 WC Agriculture, Multidisciplinary; Plant Sciences SC Agriculture; Plant Sciences GA 158FW UT WOS:000319955900002 PM 25288939 ER PT J AU Walker, AM Hayes, RP Youn, B Vermerris, W Sattler, SE Kang, C AF Walker, Alexander M. Hayes, Robert P. Youn, Buhyun Vermerris, Wilfred Sattler, Scott E. Kang, ChulHee TI Elucidation of the Structure and Reaction Mechanism of Sorghum Hydroxycinnamoyltransferase and Its Structural Relationship to Other Coenzyme A-Dependent Transferases and Synthases SO PLANT PHYSIOLOGY LA English DT Article ID ZINC-FINGER NUCLEASES; LIGNIN BIOSYNTHESIS; ARABIDOPSIS-THALIANA; BIOFUEL PRODUCTION; BAHD SUPERFAMILY; PLANTS; ACYLTRANSFERASES; DIVERSIFICATION; IDENTIFICATION; PURIFICATION AB Hydroxycinnamoyltransferase (HCT) from sorghum (Sorghum bicolor) participates in an early step of the phenylpropanoid pathway, exchanging coenzyme A (CoA) esterified to p-coumaric acid with shikimic or quinic acid as intermediates in the biosynthesis of the monolignols coniferyl alcohol and sinapyl alcohol. In order to elucidate the mode of action of this enzyme, we have determined the crystal structures of SbHCT in its apo-form and ternary complex with shikimate and p-coumaroyl-CoA, which was converted to its product during crystal soaking. The structure revealed the roles of threonine-36, serine-38, tyrosine-40, histidine-162, arginine-371, and threonine-384 in catalysis and specificity. Based on the exact chemistry of p-coumaroyl-CoA and shikimic acid in the active site and an analysis of kinetic and thermodynamic data of the wild type and mutants, we propose a role for histidine-162 and threonine-36 in the catalytic mechanism of HCT. Considering the calorimetric data, substrate binding of SbHCT should occur sequentially, with p-coumaroyl-CoA binding prior to the acyl acceptor molecule. While some HCTs can use both shikimate and quinate as an acyl acceptor, SbHCT displays low activity toward quinate. Comparison of the structure of sorghum HCT with the HCT involved in chlorogenic acid synthesis in coffee (Coffea canephora) revealed many shared features. Taken together, these observations explain how CoA-dependent transferases with similar structural features can participate in different biochemical pathways across species. C1 [Walker, Alexander M.; Youn, Buhyun; Kang, ChulHee] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Hayes, Robert P.; Kang, ChulHee] Washington State Univ, Dept Chem, Pullman, WA 99164 USA. [Vermerris, Wilfred] Univ Florida, Dept Microbiol & Cell Sci & Genet Inst, Gainesville, FL 32610 USA. [Sattler, Scott E.] USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Kang, C (reprint author), Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. EM chkang@wsu.edu FU National Science Foundation [MCB 102114]; National Research Initiative of the U.S. Department of Agriculture [35318-17454]; USDA-Biomass Research and Development Initiative [2011-10006-30358] FX This work was supported by the National Science Foundation (grant no. MCB 102114) and the National Research Initiative of the U.S. Department of Agriculture (grant no. 35318-17454). W. V. acknowledges funding from USDA-Biomass Research and Development Initiative (grant no. 2011-10006-30358). NR 45 TC 20 Z9 20 U1 2 U2 36 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2013 VL 162 IS 2 BP 640 EP 651 DI 10.1104/pp.113.217836 PG 12 WC Plant Sciences SC Plant Sciences GA 156KE UT WOS:000319819900009 PM 23624856 ER PT J AU Loraine, AE McCormick, S Estrada, A Patel, K Qin, P AF Loraine, Ann E. McCormick, Sheila Estrada, April Patel, Ketan Qin, Peng TI RNA-Seq of Arabidopsis Pollen Uncovers Novel Transcription and Alternative Splicing SO PLANT PHYSIOLOGY LA English DT Article ID GENE-EXPRESSION; CIRCADIAN CLOCK; HYPOTHESIS GENERATION; PLANT BIOLOGY; REVEALS; THALIANA; GROWTH; REGULATOR; PROTEINS; FAMILY AB Pollen grains of Arabidopsis (Arabidopsis thaliana) contain two haploid sperm cells enclosed in a haploid vegetative cell. Upon germination, the vegetative cell extrudes a pollen tube that carries the sperm to an ovule for fertilization. Knowing the identity, relative abundance, and splicing patterns of pollen transcripts will improve our understanding of pollen and allow investigation of tissue-specific splicing in plants. Most Arabidopsis pollen transcriptome studies have used the ATH1 microarray, which does not assay splice variants and lacks specific probe sets for many genes. To investigate the pollen transcriptome, we performed high-throughput sequencing (RNA-Seq) of Arabidopsis pollen and seedlings for comparison. Gene expression was more diverse in seedling, and genes involved in cell wall biogenesis were highly expressed in pollen. RNA-Seq detected at least 4,172 protein-coding genes expressed in pollen, including 289 assayed only by nonspecific probe sets. Additional exons and previously unannotated 59 and 39 untranslated regions for pollen-expressed genes were revealed. We detected regions in the genome not previously annotated as expressed; 14 were tested and 12 were confirmed by polymerase chain reaction. Gapped read alignments revealed 1,908 high-confidence new splicing events supported by 10 or more spliced read alignments. Alternative splicing patterns in pollen and seedling were highly correlated. For most alternatively spliced genes, the ratio of variants in pollen and seedling was similar, except for some encoding proteins involved in RNA splicing. This study highlights the robustness of splicing patterns in plants and the importance of ongoing annotation and visualization of RNA-Seq data using interactive tools such as Integrated Genome Browser. C1 [Loraine, Ann E.; Estrada, April; Patel, Ketan] Univ N Carolina, Dept Bioinformat & Genom, Kannapolis, NC 28081 USA. [McCormick, Sheila; Qin, Peng] Univ Calif Berkeley, USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Loraine, AE (reprint author), Univ N Carolina, Dept Bioinformat & Genom, Kannapolis, NC 28081 USA. EM aloraine@uncc.edu FU National Science Foundation Research Coordination Network for Integrative Pollen Biology [MCB-0955431]; U.S. Department of Agriculture Current Research Information System [5335-21000-030-00D]; National Science Foundation Arabidopsis [0820371]; University of North Carolina-Charlotte; University of North Carolina General Administration FX This work was supported by a travel award from the National Science Foundation Research Coordination Network for Integrative Pollen Biology (grant no. MCB-0955431 to A. L.) and by the U.S. Department of Agriculture Current Research Information System (5335-21000-030-00D to S. M.). Integrated Genome Browser software used in the study was supported by National Science Foundation Arabidopsis 2010 (grant no. 0820371 to A. L.). A. E. and K. P. received salary support from startup funds provided by University of North Carolina-Charlotte and University of North Carolina General Administration. NR 37 TC 70 Z9 72 U1 3 U2 67 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 J9 PLANT PHYSIOL JI Plant Physiol. PD JUN PY 2013 VL 162 IS 2 BP 1092 EP 1109 DI 10.1104/pp.112.211441 PG 18 WC Plant Sciences SC Plant Sciences GA 156KE UT WOS:000319819900043 PM 23590974 ER PT J AU Jiang, S Cheng, HW Cui, LY Zhou, ZL Hou, JF AF Jiang, S. Cheng, H. W. Cui, L. Y. Zhou, Z. L. Hou, J. F. TI Changes of blood parameters associated with bone remodeling following experimentally induced fatty liver disorder in laying hens SO POULTRY SCIENCE LA English DT Article DE osteocalcin; fatty liver disorder; nutrition; osteoporosis; hen ID PROSTAGLANDIN E-2 PRODUCTION; K-DEPENDENT PROTEIN; DIETARY LIPIDS; HEMORRHAGIC SYNDROME; METABOLIC DISEASES; AVIAN OSTEOPOROSIS; ENERGY-METABOLISM; MINERAL CONTENT; JAPANESE-QUAIL; CHICKEN LEPTIN AB Studies have demonstrated that obesity and osteoporosis are linked disorders in humans. This study examined the hypothesis that excessive lipid consumption affects bone metabolism in laying hens. A total of one hundred 63-wk-old laying hens were randomly divided into 2 treatments and fed either a regular layer diet (control) or a high energy and low protein diet (HE-LP; experimental treatment) for 80 d. Egg production, feed intake, and BW were recorded at various days during the treatment. At d 80, ten randomly chosen birds per treatment group were killed. Abdominal fat weight, liver weight, and liver fat content were determined. Serum levels of total calcium, inorganic phosphate, and alkaline phosphatase were measured using a biochemical analyzer. Serum concentrations of osteocalcin, leptin-like protein, and estrogen were measured by enzyme-linked immunosorbent assay. Tibia length and width were measured using a vernier caliper; density of the right tibias was determined using an x-ray scanner; and mechanical properties of the left tibias were analyzed using a material testing machine. The expression of osteocalcin and osteoprotegerin mRNA in the keel bone was analyzed by real-time PCR. The concentration of osteocalcin protein in the keels was measured using western blot. Compared with control hens, hens fed the HE-LP diet had lower egg production, lower feed intake, greater liver fat content, and greater abdominal fat pad mass (P < 0.05). Feeding the HE-LP diet increased serum alkaline phosphatase activity, osteocalcin, leptin-like protein, and estrogen concentrations (P < 0.05), and decreased the keel osteocalcin concentrations (P < 0.05). There were significant positive correlations between the serum concentrations of leptin-like protein, estrogen, and osteocalcin regardless of treatment (P < 0.05). The results indicated that HE-LP diet induced a fatty liver disorder in laying hens with an upregulation in bone turnover and exacerbated skeletal damage. The data supported a role for lipid metabolism in skeletal heath of laying hens. C1 [Jiang, S.; Cui, L. Y.; Zhou, Z. L.; Hou, J. F.] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China. [Cheng, H. W.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Hou, JF (reprint author), Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China. EM jfhou@njau.edu.cn RI Wilkinson, Stuart/C-2802-2013 FU Program for Post-graduates Research Innovation of University of Jiangsu Province [CXZZ11-0681]; National Natural Science Foundation of China [30972234, 31272618]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) FX This work was supported by the Program for Post-graduates Research Innovation of University of Jiangsu Province (CXZZ11-0681), National Natural Science Foundation of China (No. 30972234 and 31272618), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). NR 69 TC 9 Z9 9 U1 2 U2 36 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUN PY 2013 VL 92 IS 6 BP 1443 EP 1453 DI 10.3382/ps.2012-02800 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 149MB UT WOS:000319322900002 PM 23687138 ER PT J AU Yuan, ZG Luo, SJ Dubey, JP Zhou, DH Zhu, YP He, Y He, XH Zhang, XX Zhu, XQ AF Yuan, Zi-Guo Luo, Sheng-Jun Dubey, Jitender P. Zhou, Dong-Hui Zhu, Yan-Ping He, Yong He, Xian-Hui Zhang, Xiu-Xiang Zhu, Xing-Quan TI Serological Evidence of Toxoplasma gondii Infection in Five Species of Bats in China SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Toxoplasma gondii; Toxoplasmosis; Bat; China; Seroprevalence; Modified agglutination test ID SPECIFICITY; WILDLIFE; PIGS AB Toxoplasma gondii is an obligate intracellular protozoan parasite that can infect almost all warm-blooded animals and humans with a worldwide distribution. Bats are reservoirs for an increasing number of emerging zoonotic viruses, such as henipaviruses and severe acute respiratory syndrome coronavirus (SARS-CoV). However, little is known of T. gondii infection in bats. The objective of the present study was to determine the seroprevalence of T. gondii infection in bats in China. A total of 217 serum samples from 5 species of bats were collected between April, 2010, and August, 2011, from 4 provinces in China. Antibodies to T. gondii were determined using the modified agglutination test (MAT, 1:25 or higher). Antibodies to T. gondii were found in 26.5% (18/68) Megaderma lyra, 13.6% (12/88) Rousettus leschenaulti, 13.6% (3/22) Cynopterus sphinx, 20% (4/20) Vespertilio superaus, and 15.8% (3/19) Pipistrellus javanicus. Antibody titers ranged from 1:25 to 1:400, with titers of 1:200 detected in 4 of the 5 bat species. The present study suggests the likely occurrence of T. gondii infection in bats in China, and these bats are new putative hosts for T. gondii, which may pose a threat to human health. C1 [Yuan, Zi-Guo; Zhou, Dong-Hui; Zhang, Xiu-Xiang; Zhu, Xing-Quan] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou 730046, Gansu, Peoples R China. [Yuan, Zi-Guo; He, Xian-Hui] South China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China. [Luo, Sheng-Jun] Guangdong Acad Agr Sci, Inst Vet Med, Guangzhou, Guangdong, Peoples R China. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Anim & Natl Resources Inst, USDA, Beltsville, MD USA. [Zhu, Yan-Ping; He, Yong] Xinxiang Univ, Dept Life Sci & Technol, Xinxiang, Henan Province, Peoples R China. [Zhang, Xiu-Xiang] South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China. [Zhu, Xing-Quan] Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming, Yunnan Province, Peoples R China. RP Zhu, XQ (reprint author), Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China. EM xiuxiangzh@scau.edu.cn; xingquanzhu1@hotmail.com FU Project of Science and Technology New Star of Zhu Jiang [2011J2200100]; National Natural Science Foundation of China [30901067, 31230073, 31172316, 31101812]; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences [SKLVEB2009KFKT014, SKLVEB2010 KFKT010, SKLVEB2011KFKT004]; Specialized Research Fund for the Doctoral Program of Higher Education [20094404120016]; Yunnan Provincial Program for Introducing High-level Scientists [2009CI125] FX This work is supported, in part, by grants from the Project of Science and Technology New Star of Zhu Jiang (2011J2200100), the National Natural Science Foundation of China (grant nos. 30901067, 31230073, 31172316, and 31101812), the Open Funds of the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (SKLVEB2009KFKT014, SKLVEB2010 KFKT010, and SKLVEB2011KFKT004), the Specialized Research Fund for the Doctoral Program of Higher Education (grant no. 20094404120016), and the Yunnan Provincial Program for Introducing High-level Scientists (grant no. 2009CI125). NR 13 TC 10 Z9 10 U1 0 U2 14 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN PY 2013 VL 13 IS 6 BP 422 EP 424 DI 10.1089/vbz.2012.1091 PG 3 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 157NV UT WOS:000319905800013 PM 23473226 ER PT J AU Van Pelt, RS AF Van Pelt, R. Scott TI Use of anthropogenic radioisotopes to estimate rates of soil redistribution by wind I: Historic use of Cs-137 SO AEOLIAN RESEARCH LA English DT Review DE Soil redistribution; Wind erosion; Radioisotopes; Global fallout; Cs-137; Spatial variability ID QINGHAI-TIBET PLATEAU; EROSION RATES; SPATIAL VARIABILITY; FALLOUT CS-137; SEDIMENT REDISTRIBUTION; CESIUM-137 MEASUREMENTS; SOUTHWESTERN NIGER; PLUTONIUM ISOTOPES; CULTIVATED SOILS; NULLARBOR PLAIN AB Wind erosion is increasingly scrutinized as a causative factor in soil degradation and fugitive dust emissions. Although models have been developed to predict wind erosion and dust emissions, they are not accurate in all locations. The temporal and spatial variability of aeolian processes makes local estimates of long-term average erosion costly and time consuming. Atmospheric testing of nuclear weapons during the 1950s and 1960s resulted in anthropogenic radioisotopes that had not previously existed being injected into stratospheric global circulation and subsequently deposited on the Earth's surface. Many of these radioisotopes are strongly adsorbed to soil particles and their movement on the landscape is a powerful method for investigating soil redistribution by wind, water, and tillage. Cs-137 is the most commonly used anthropogenic radioisotope used to assess soil redistribution rates. Models have been developed to equate differences of radioisotope inventories with rates of soil redistribution and these models have been employed globally to assess soil redistribution on agricultural and natural landscapes. The radioisotope method for assessing soil redistribution rates has many advantages, but also a few limitations. One of the major limitations occurs when local sources of radioisotope contamination, particularly Cs-137, mask the pulse from global fallout, making temporal estimates of redistribution difficult or impossible. In this paper, I explore the importance, history, and applications of the radioisotopic technique using Cs-137, particularly as it applies to soil redistribution by wind. Published by Elsevier B.V. C1 USDA ARS, Wind Eros & Water Conservat Res Unit, Big Spring, TX 79720 USA. RP Van Pelt, RS (reprint author), USDA ARS, Wind Eros & Water Conservat Res Unit, Big Spring, TX 79720 USA. EM scott.vanpelt@ars.usda.gov NR 188 TC 7 Z9 7 U1 3 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD JUN PY 2013 VL 9 BP 89 EP 102 DI 10.1016/j.aeolia.2012.11.004 PG 14 WC Geography, Physical SC Physical Geography GA 153WF UT WOS:000319632500007 ER PT J AU Van Pelt, RS Ketterer, ME AF Van Pelt, R. Scott Ketterer, Michael E. TI Use of anthropogenic radioisotopes to estimate rates of soil redistribution by wind II: The potential for future use of Pu239+240 SO AEOLIAN RESEARCH LA English DT Review DE Soil redistribution; Wind erosion; Radioisotopes; Global fallout; Cs-137; Pu239+240 ID PLASMA-MASS SPECTROMETRY; ORGANIC-MATTER; FALLOUT PU-239+240; GLOBAL FALLOUT; GRASSLAND SOIL; TERRESTRIAL ENVIRONMENT; MARSHALL-ISLANDS; ROCKY FLATS; PLUTONIUM; CS-137 AB In the previous paper, the use of soilborne Cs-137 from atmospheric fallout to estimate rates of soil redistribution, particularly by wind, was reviewed. This method relies on the assumption that the source of Cs-137 in the soil profile is from atmospheric fallout following the period of atmospheric weapons testing so that the temporal and, to a certain extent, the spatial patterns of Cs-137 deposition are known. One of the major limitations occurs when local or regional sources of Cs-137 contamination mask the pulse from global fallout, making temporal estimates of redistribution difficult or impossible. Like Cs-137, Pu exhibits strong affinity for binding to soil particle surfaces, and therefore, re-distribution of Pu inventory indicates inferred soil re-distribution. Compared to Cs-137, Pu-239 and Pu-240 offer several important advantages: (a) the two major Pu isotopes have much longer half-lives than Cs-137 and (b) the ratio Pu-240/Pu-239 is used to examine whether the Pu is from stratospheric fallout. In this paper, we review the literature concerning Pu in soil and of current attempts to use this tracer to estimate rates of soil redistribution. We also present preliminary, unpublished data from a pilot study designed to test whether or not Pu239+240 can be used to estimate rates of soil redistribution by wind. Based on similarities of profile distribution and relative inventories between Cs-137 measurements and Pu239+240 measurements of split samples from a series of fields with documented wind erosion histories, we conclude that Pu239+240 may well be the anthropogenic radioisotope of choice for future soil redistribution investigations. Published by Elsevier B.V. C1 [Van Pelt, R. Scott] USDA ARS, Wind Eros & Water Conservat Res Unit, Big Spring, TX 79720 USA. [Ketterer, Michael E.] No Arizona Univ, Dept Chem & Biochem, Flagstaff, AZ 86011 USA. RP Van Pelt, RS (reprint author), USDA ARS, Wind Eros & Water Conservat Res Unit, Big Spring, TX 79720 USA. EM scott.vanpelt@ars.usda.gov NR 62 TC 6 Z9 6 U1 2 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 J9 AEOLIAN RES JI Aeolian Res. PD JUN PY 2013 VL 9 BP 103 EP 110 DI 10.1016/j.aeolia.2013.01.004 PG 8 WC Geography, Physical SC Physical Geography GA 153WF UT WOS:000319632500008 ER PT J AU Sharratt, BS Vaddella, VK Feng, G AF Sharratt, B. S. Vaddella, V. K. Feng, G. TI Threshold friction velocity influenced by wetness of soils within the Columbia Plateau SO AEOLIAN RESEARCH LA English DT Article DE Wind erosion; Dust; PM10; Soil matric potential; Soil water content ID AIR-QUALITY; AGRICULTURAL FIELDS; WINDBLOWN DUST; WIND-TUNNEL; ENTRAINMENT; SEDIMENT; EROSION; MOISTURE; MODEL; PM10 AB Windblown dust impacts air quality in the Columbia Plateau of the US Pacific Northwest. Wind erosion of agricultural lands, which is the predominate source of windblown dust in the region, occurs when the friction velocity exceeds the threshold friction velocity (u(*t)) of the surface. Soil moisture influences the u(*t), but the u(*t) of variably wet soils is virtually unknown in the region. The relationship between u(*t) and water content was ascertained for five silt loams collected from field sites in eastern Washington. After moistening the soils to predetermined water contents, u(*t) was assessed from wind velocity and PM10 (particles with an aerodynamic diameter <= 10 mu m) and TSP (total suspended particulate matter) concentration profiles above the soil surface inside a wind tunnel. Soil water content appeared to have little influence on until gravimetric water content rose to 6-14% (depending on the soil). Trends in the relationship between u(*t) and soil matric potential (Psi) suggested moistening soils had little influence on u(*t) until the Psi was in the range of -25 to -1 MPa. The ut rose sharply with an increase in Psi above -1 MPa. The results of this study suggest that u(*t) is significantly influenced by adsorption and capillary forces that comprise the matric potential of soils. Published by Elsevier B.V. C1 [Sharratt, B. S.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Vaddella, V. K.] Texas A&M Univ Syst, Texas AgriLife Res, Amarillo, TX 79106 USA. [Feng, G.] Chinese Acad Sci, State Key Lab Desert & Oasis Ecol, Xinjiang Inst Ecol & Geog, Urumqi 830011, Xinjiang, Peoples R China. [Feng, G.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Sharratt, BS (reprint author), Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM Brenton.Sharratt@ars.usda.gov FU USDA-CSREES Columbia Plateau Wind Erosion/Air Quality project; National Natural Science Foundation of China [41171019]; Chinese Academy of Sciences FX This research was supported by the USDA-CSREES Columbia Plateau Wind Erosion/Air Quality project, the National Natural Science Foundation of China Project (Grant No. 41171019), and One Hundred Talented Researchers Program of Chinese Academy of Sciences. NR 27 TC 5 Z9 6 U1 1 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1875-9637 EI 2212-1684 J9 AEOLIAN RES JI Aeolian Res. PD JUN PY 2013 VL 9 BP 175 EP 182 DI 10.1016/j.aeolia.2013.01.002 PG 8 WC Geography, Physical SC Physical Geography GA 153WF UT WOS:000319632500014 ER PT J AU Dominguez-Salas, P Moore, SE Cole, D da Costa, KA Cox, SE Dyer, RA Fulford, AJC Innis, SM Waterland, RA Zeisel, SH Prentice, AM Hennig, BJ AF Dominguez-Salas, Paula Moore, Sophie E. Cole, Darren da Costa, Kerry-Ann Cox, Sharon E. Dyer, Roger A. Fulford, Anthony J. C. Innis, Sheila M. Waterland, Robert A. Zeisel, Steven H. Prentice, Andrew M. Hennig, Branwen J. TI DNA methylation potential: dietary intake and blood concentrations of one-carbon metabolites and cofactors in rural African women SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID S-ADENOSYLHOMOCYSTEINE; GAMBIAN WOMEN; MICRONUTRIENT SUPPLEMENTATION; MASS-SPECTROMETRY; RIBOFLAVIN STATUS; CYSTIC-FIBROSIS; HOMOCYSTEINE; CHOLINE; FOLATE; METHIONINE AB Background: Animal models show that periconceptional supplementation with folic acid, vitamin B-12, choline, and betaine can induce differences in offspring phenotype mediated by epigenetic changes in DNA. In humans, altered DNA methylation patterns have been observed in offspring whose mothers were exposed to famine or who conceived in the Gambian rainy season. Objective: The objective was to understand the seasonality of DNA methylation patterns in rural Gambian women. We studied natural variations in dietary intake of nutrients involved in methyl-donor pathways and their effect on the respective metabolic biomarkers. Design: In 30 women of reproductive age (18-45 y), we monitored diets monthly for 1 y by using 48-h weighed records to measure intakes of choline, betaine, folate, methionine, riboflavin, and vitamins B-6 and B-12. Blood biomarkers of these nutrients, S-adenosylhomocysteine (SAH), S-adenosylmethionine (SAM), homocysteine, cysteine, and dimethylglycine were also assessed monthly. Results: Dietary intakes of riboflavin, folate, choline, and betaine varied significantly by season; the most dramatic variation was seen for betaine. All metabolic biomarkers showed significant seasonality, and vitamin B-6 and folate had the highest fluctuations. Correlations between dietary intakes and blood biomarkers were found for riboflavin, vitamin B-6, active vitamin B-12 (holotranscobalamin), and betaine. We observed a seasonal switch between the betaine and folate pathways and a probable limiting role of riboflavin in these processes and a higher SAM/SAH ratio during the rainy season. Conclusions: Naturally occurring seasonal variations in food-consumption patterns have a profound effect on methyl-donor biomarker status. The direction of these changes was consistent with previously reported differences in methylation of metastable epi-alleles. C1 [Dominguez-Salas, Paula; Moore, Sophie E.; Cox, Sharon E.; Fulford, Anthony J. C.; Prentice, Andrew M.; Hennig, Branwen J.] Univ London London Sch Hyg & Trop Med, Int Nutr Grp, MRC, London WC1E 7HT, England. [Dominguez-Salas, Paula; Moore, Sophie E.; Fulford, Anthony J. C.; Prentice, Andrew M.] MRC Unit, MRC Keneba, Keneba, Gambia. [Cole, Darren] Elsie Widdowson Lab, MRC Human Nutr Res, Cambridge, England. [da Costa, Kerry-Ann; Zeisel, Steven H.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Nutr, Chapel Hill, NC USA. [da Costa, Kerry-Ann; Zeisel, Steven H.] Univ N Carolina, Sch Med, Chapel Hill, NC USA. [Dyer, Roger A.; Innis, Sheila M.] Univ British Columbia, Dept Pediat, Vancouver, BC V6T 1W5, Canada. [Waterland, Robert A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, Houston, TX 77030 USA. [Waterland, Robert A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Dominguez-Salas, P (reprint author), Univ London London Sch Hyg & Trop Med, Int Nutr Grp, MRC, Keppel St, London WC1E 7HT, England. EM paula.dominguez-salas@lshtm.ac.uk RI Hennig, Branwen/M-6444-2014; OI Cox, Sharon/0000-0002-9908-2936 FU Wellcome Trust grant [WT086369MA]; MRC core funding [MC-A760-5QX00]; Bill and Melinda Gates Foundation; NIH [P30DK056350]; NIH/National Institute of Diabetes and Digestive and Kidney Diseases [1R01DK081557]; USDA [CRIS 6250-51000-055] FX Supported by Wellcome Trust grant WT086369MA (to BJH) and MRC core funding MC-A760-5QX00 (to the International Nutrition Group). SHZ and K-AdC were supported in part by a grant from the Bill and Melinda Gates Foundation and from the NIH (P30DK056350) to the University of North Carolina-CH Nutrition Obesity Research Center. RAW was supported by grants from NIH/National Institute of Diabetes and Digestive and Kidney Diseases (1R01DK081557) and the USDA (CRIS 6250-51000-055). NR 43 TC 43 Z9 43 U1 0 U2 22 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2013 VL 97 IS 6 BP 1217 EP 1227 DI 10.3945/ajcn.112.048462 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 150DX UT WOS:000319371500010 PM 23576045 ER PT J AU Dwyer, J Nahin, RL Rogers, GT Barnes, PM Jacques, PM Sempos, CT Bailey, R AF Dwyer, Johanna Nahin, Richard L. Rogers, Gail T. Barnes, Patricia M. Jacques, Paul M. Sempos, Christopher T. Bailey, Regan TI Prevalence and predictors of children's dietary supplement use: the 2007 National Health Interview Survey SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID ALTERNATIVE MEDICINE; UNITED-STATES; COMPLEMENTARY; ADULTS; ADOLESCENTS; VITAMIN; INFANTS; FOODS; HERB AB Background: Little is known about the characteristics of US children who are dietary supplement users. Objective: We described the prevalence and predictors of and reasons for giving children dietary supplements. Design: The study included children <18 y of age who participated in the Complementary and Alternative Medicine supplement of the National Health Interview Survey of 2007 whose proxies provided complete information on child dietary supplement use. Results: A total of 37% of subjects used dietary supplements, 31% of subjects used multivitamin mineral (MVM) products exclusively, 4% of subjects used single vitamins or minerals solely or in combination with MVMs, and 2% of subjects used nonvitamin, nonmineral products either solely or in combination with other supplements. Users were more likely than nonusers to be Asian, white, or non-Hispanic; belong to families with higher parental education and income levels; reside in areas other than the South; be in good, very good, or excellent health; have private health insurance; and have a usual place at which they received conventional medical care. Children (3%) with the most disease burden and health care were more likely to use supplements than were healthier children. Supplements were given for the prevention or treatment of many illnesses and conditions. Neither the caregiver's reasons nor specific supplements used were consistently associated with particular conditions. Conclusions: The 37% of US children who used any type of dietary supplements differed from nonusers in family socioeconomic status and many other health-related characteristics. Users were given supplements to prevent or treat many illnesses and conditions for which there is only limited evidence of their efficacy. C1 [Dwyer, Johanna; Sempos, Christopher T.; Bailey, Regan] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Nahin, Richard L.] NIH, Natl Ctr Complementary & Alternat Med, Bethesda, MD 20892 USA. [Dwyer, Johanna; Rogers, Gail T.; Jacques, Paul M.; Bailey, Regan] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Barnes, Patricia M.] Natl Ctr Hlth Stat, Res Data Ctr, Hyattsville, MD 20782 USA. RP Dwyer, J (reprint author), Off Dietary Supplements, 6100 Execut Blvd, Bethesda, MD 20008 USA. EM dwyerj1@od.nih.gov OI Nahin, Richard/0000-0002-3682-4816; Dwyer, Johanna/0000-0002-0783-1769 FU National Center for Complementary and Alternative Medicine; Office of Dietary Supplements, NIH; National Center for Health Statistics of the CDC; US Department of Health and Human Services; USDA, Agricultural Research Service [58-1950-7-707]; National Heart, Lung, and Blood Institute at NIH; National Institute of Allergy and Infectious Diseases at NIH; Eunice Kennedy Shriver National Institute of Child Health and Human Development at NIH; National Institute of Mental Health at NIH; Office of Behavioral and Social Science Research at NIH; National Center for Complementary and Alternative Medicine at NIH FX Supported by the National Center for Complementary and Alternative Medicine, the Office of Dietary Supplements, NIH, the National Center for Health Statistics of the CDC, US Department of Health and Human Services, and the USDA, Agricultural Research Service (agreement 58-1950-7-707). The 2007 Adult and Child Complementary and Alternative Medicine supplement of the National Health Interview Survey was supported by 7 institutes, centers, and offices at the NIH, including the National Center for Complementary and Alternative Medicine; the National Heart, Lung, and Blood Institute; the National Institute of Allergy and Infectious Diseases; the Eunice Kennedy Shriver National Institute of Child Health and Human Development; the National Institute of Mental Health; the Office of Behavioral and Social Science Research; and the Office of Dietary Supplements, NIH. NR 37 TC 24 Z9 24 U1 0 U2 12 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2013 VL 97 IS 6 BP 1331 EP 1337 DI 10.3945/ajcn.112.052373 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 150DX UT WOS:000319371500023 PM 23576049 ER PT J AU Tanaka, T Ngwa, JS van Rooij, FJA Zillikens, MC Wojczynski, MK Frazier-Wood, AC Houston, DK Kanoni, S Lemaitre, RN Luan, J Mikkila, V Renstrom, F Sonestedt, E Zhao, JH Chu, AY Qi, L Chasman, DI Otto, MCD Dhurandhar, EJ Feitosa, MF Johansson, I Khaw, KT Lohman, KK Manichaikul, A McKeown, NM Mozaffarian, D Singleton, A Stirrups, K Viikari, J Ye, Z Bandinelli, S Barroso, I Deloukas, P Forouhi, NG Hofman, A Liu, YM Lyytikainen, LP North, KE Dimitriou, M Hallmans, G Kahonen, M Langenberg, C Ordovas, JM Uitterlinden, AG Hu, FB Kalafati, IP Raitakari, O Franco, OH Johnson, A Emilsson, V Schrack, JA Semba, RD Siscovick, DS Arnett, DK Borecki, IB Franks, PW Kritchevsky, SB Lehtimaki, T Loos, RJF Orho-Melander, M Rotter, JI Wareham, NJ Witteman, JCM Ferrucci, L Dedoussis, G Cupples, LA Nettleton, JA AF Tanaka, Toshiko Ngwa, Julius S. van Rooij, Frank J. A. Zillikens, M. Carola Wojczynski, Mary K. Frazier-Wood, Alexis C. Houston, Denise K. Kanoni, Stavroula Lemaitre, Rozenn N. Luan, Jian'an Mikkila, Vera Renstrom, Frida Sonestedt, Emily Zhao, Jing Hua Chu, Audrey Y. Qi, Lu Chasman, Daniel I. Otto, Marcia C. de Oliveira Dhurandhar, Emily J. Feitosa, Mary F. Johansson, Ingegerd Khaw, Kay-Tee Lohman, Kurt K. Manichaikul, Ani McKeown, Nicola M. Mozaffarian, Dariush Singleton, Andrew Stirrups, Kathleen Viikari, Jorma Ye, Zheng Bandinelli, Stefania Barroso, Ines Deloukas, Panos Forouhi, Nita G. Hofman, Albert Liu, Yongmei Lyytikainen, Leo-Pekka North, Kari E. Dimitriou, Maria Hallmans, Goran Kahonen, Mika Langenberg, Claudia Ordovas, Jose M. Uitterlinden, Andre G. Hu, Frank B. Kalafati, Ioanna-Panagiota Raitakari, Olli Franco, Oscar H. Johnson, Andrew Emilsson, Valur Schrack, Jennifer A. Semba, Richard D. Siscovick, David S. Arnett, Donna K. Borecki, Ingrid B. Franks, Paul W. Kritchevsky, Stephen B. Lehtimaki, Terho Loos, Ruth J. F. Orho-Melander, Marju Rotter, Jerome I. Wareham, Nicholas J. Witteman, Jacqueline C. M. Ferrucci, Luigi Dedoussis, George Cupples, L. Adrienne Nettleton, Jennifer A. TI Genome-wide meta-analysis of observational studies shows common genetic variants associated with macronutrient intake SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID QUANTITATIVE TRAIT LOCUS; 5-HT2A RECEPTOR GENE; FTO GENE; ENERGY-EXPENDITURE; FOOD-INTAKE; PPAR-ALPHA; METABOLIC REGULATOR; DIETARY-INTAKE; ADULT OBESITY; CHILDREN AB Background: Macronutrient intake varies substantially between individuals, and there is evidence that this variation is partly accounted for by genetic variants. Objective: The objective of the study was to identify common genetic variants that are associated with macronutrient intake. Design: We performed 2-stage genome-wide association (GWA) meta-analysis of macronutrient intake in populations of European descent. Macronutrients were assessed by using food-frequency questionnaires and analyzed as percentages of total energy consumption from total fat, protein, and carbohydrate. From the discovery GWA (n = 38,360), 35 independent loci associated with macronutrient intake at P < 5 x 10(-6) were identified and taken forward to replication in 3 additional cohorts (n = 33,533) from the DietGen Consortium. For one locus, fat mass obesity-associated protein (FTO), cohorts with Illumina MetaboChip genotype data (n 7724) provided additional replication data. Results: A variant in the chromosome 19 locus (rs838145) was associated with higher carbohydrate (beta +/- SE: 0.25 +/- 0.04%; P = 1.68 x 10(-8)) and lower fat (beta = SE: -0.21 +/- 0.04%; P = 1.57 x 10(-9)) consumption. A candidate gene in this region, fibroblast growth factor 21 (FGF21), encodes a fibroblast growth factor involved in glucose and lipid metabolism. The variants in this locus were associated with circulating FGF21 protein concentrations (P < 0.05) but not mRNA concentrations in blood or brain. The body mass index (BMI) increasing allele of the FTO variant (rs1421085) was associated with higher protein intake (beta +/- SE: 0.10 +/- 0.02%; P = 9.96 x 10(-10)), independent of BMI (after adjustment for BMI, beta +/- SE: 0.08 +/- 0.02%; P = 3.15 x 10(-7)). Conclusion: Our results indicate that variants in genes involved in nutrient metabolism and obesity are associated with macronutrient consumption in humans. Trials related to this study were registered at clinicaltrials.gov as NCT00005131 (Atherosclerosis Risk in Communities), NCT00005133 (Cardiovascular Health Study), NCT00005136 (Family Heart Study), NCT00005121 (Framingham Heart Study), NCT00083369 (Genetic and Environmental Determinants of Triglycerides), NCT01331512 (InCHIANTI Study), and NCT00005487 (Multi-Ethnic Study of Atherosclerosis). C1 [Schrack, Jennifer A.; Ferrucci, Luigi] NIA, Translat Gerontol Branch, Baltimore, MD 21225 USA. [Ngwa, Julius S.; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [van Rooij, Frank J. A.; Hofman, Albert; Uitterlinden, Andre G.; Franco, Oscar H.; Witteman, Jacqueline C. M.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [van Rooij, Frank J. A.; Zillikens, M. Carola; Hofman, Albert; Uitterlinden, Andre G.; Franco, Oscar H.; Witteman, Jacqueline C. M.] Netherlands Genom Initiat, Leiden, Netherlands. [Zillikens, M. Carola; Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Wojczynski, Mary K.; Feitosa, Mary F.; Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Frazier-Wood, Alexis C.; Dhurandhar, Emily J.] Univ Alabama Birmingham, Dept Epidemiol, Sect Stat Genet, Birmingham, AL USA. [Frazier-Wood, Alexis C.; Dhurandhar, Emily J.] Univ Alabama Birmingham, Off Energet, Birmingham, AL USA. [Houston, Denise K.; Kritchevsky, Stephen B.] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. [Lohman, Kurt K.] Wake Forest Sch Med, Dept Biostat Sci, Div Publ Hlth Sci, Winston Salem, NC USA. [Liu, Yongmei] Wake Forest Sch Med, Dept Epidemiol & Prevent, Div Publ Hlth Sci, Winston Salem, NC USA. [Kanoni, Stavroula; Stirrups, Kathleen; Barroso, Ines; Deloukas, Panos] Wellcome Trust Sanger Inst, Hinxton, England. [Lemaitre, Rozenn N.] Univ Washington, Dept Med, Seattle, WA USA. [Luan, Jian'an; Zhao, Jing Hua; Ye, Zheng; Forouhi, Nita G.; Langenberg, Claudia; Wareham, Nicholas J.] Addenbrookes Hosp, Inst Metab Sci, Epidemiol Unit, MRC, Cambridge, England. [Loos, Ruth J. F.] Addenbrookes Hosp, Inst Med Sci, Cambridge, England. [Mikkila, Vera] Univ Helsinki, Dept Food & Environm Sci, Div Nutr, Helsinki, Finland. [Renstrom, Frida; Mozaffarian, Dariush; Hu, Frank B.; Franks, Paul W.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Sonestedt, Emily; Orho-Melander, Marju] Lund Univ, Dept Clin Sci, Malmo, Sweden. [Renstrom, Frida; Franks, Paul W.] Lund Univ, Genet & Mol Epidemiol Unit, Malmo, Sweden. [Chu, Audrey Y.; Chasman, Daniel I.] Brigham & Womens Hosp, Div Prevent Med, Boston, MA 02115 USA. [Qi, Lu; Mozaffarian, Dariush; Hu, Frank B.] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA USA. [Qi, Lu; Chasman, Daniel I.; Mozaffarian, Dariush; Hu, Frank B.] Harvard Univ, Sch Med, Boston, MA USA. [Otto, Marcia C. de Oliveira; Mozaffarian, Dariush] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Johansson, Ingegerd] Umea Univ, Dept Odontol, Umea, Sweden. [Franks, Paul W.] Umea Univ, Dept Publ Hlth & Clin Med, Umea, Sweden. [Hallmans, Goran] Umea Univ, Dept Nutr Res, Umea, Sweden. [Khaw, Kay-Tee] Univ Cambridge, Inst Publ Hlth, Dept Publ Hlth & Primary Care, Cambridge, England. [Manichaikul, Ani] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [Manichaikul, Ani] Univ Virginia, Div Biostat & Epidemiol, Charlottesville, VA USA. [McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Mozaffarian, Dariush; Hu, Frank B.] Brigham & Womens Hosp, Div Cardiovasc Med, Boston, MA 02115 USA. [Singleton, Andrew] NIA, Neurogenet Lab, Bethesda, MD 20892 USA. [Viikari, Jorma] Univ Turku, Dept Med, Turku, Finland. [Viikari, Jorma] Turku Univ Hosp, FIN-20520 Turku, Finland. [Bandinelli, Stefania] Azienda Sanitaria Firenze, Geriatr Unit, Florence, Italy. [Barroso, Ines] Univ Cambridge, Addenbrookes Hosp, Metab Res Lab, Inst Metab Sci, Cambridge CB2 2QQ, England. [Lyytikainen, Leo-Pekka; Lehtimaki, Terho] Tampere Univ Hosp, Dept Clin Chem, Fimlab Labs, FIN-33521 Tampere, Finland. [Lyytikainen, Leo-Pekka; Kahonen, Mika; Lehtimaki, Terho] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland. [North, Kari E.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [North, Kari E.] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA. [Dimitriou, Maria; Kalafati, Ioanna-Panagiota; Dedoussis, George] Harokopio Univ, Dept Nutr Dietet, Athens, Greece. [Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere, Finland. [Ordovas, Jose M.] CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. [Raitakari, Olli] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Raitakari, Olli] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, FIN-20520 Turku, Finland. [Johnson, Andrew; Cupples, L. Adrienne] NHLBI, Framingham Heart Study, Framingham, MA USA. [Emilsson, Valur] Iceland Heart Assoc, Kopavogur, Iceland. [Semba, Richard D.] Johns Hopkins Univ, Sch Med, Dept Ophthalmol, Baltimore, MD 21205 USA. [Siscovick, David S.] Univ Washington, Dept Med, Seattle, WA USA. [Siscovick, David S.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Rotter, Jerome I.] Cedars Sinai Med Ctr, Inst Med Genet, Los Angeles, CA 90048 USA. [Nettleton, Jennifer A.] Univ Texas Hlth Sci Ctr Houston, Div Epidemiol Human Genet & Environm Sci, Houston, TX USA. RP Tanaka, T (reprint author), NIA, Translat Gerontol Branch, 3001 South Hanover St, Baltimore, MD 21225 USA. EM tanakato@mail.nih.gov RI Deloukas, Panos/B-2922-2013; Singleton, Andrew/C-3010-2009; Lyytikainen, Leo-Pekka/C-8544-2016; Sonestedt, Emily/I-3814-2016; Johnson, Andrew/G-6520-2013; Feitosa, Mary/K-8044-2012; Wood, Lekki/B-8053-2010; OI Deloukas, Panos/0000-0001-9251-070X; Forouhi, Nita/0000-0002-5041-248X; Lyytikainen, Leo-Pekka/0000-0002-7200-5455; Sonestedt, Emily/0000-0002-0747-4562; Feitosa, Mary/0000-0002-0933-2410; Wood, Lekki/0000-0001-7616-2119; Cupples, L. Adrienne/0000-0003-0273-7965; Kritchevsky, Stephen/0000-0003-3336-6781; Franks, Paul/0000-0002-0520-7604 FU National Institutes of Health; NIH Roadmap for Medical Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Neurological Disorders and Stroke; National Institute on Aging; National Center of Advancing Translational Technologies; US Department of Agriculture; Medical Research Council UK; Wellcome Trust; Italian Ministry of Health; Netherlands Organisation of Scientific Research NWO Investments; Research Institute for Diseases in the Elderly (RIDE2) [014-93-015]; Netherlands Genomics Initiative (NGI)/Netherlands Consortium for Healthy Aging (NCHA), Rotterdam; Netherlands Organization for the Health Research and Development; Research Institute for Diseases in the Elderly (RIDE); Ministry of Education, Culture, and Science; Ministry for Health, Welfare, and Sports; European Commission; Municipality of Rotterdam; Academy of Finland; Social Insurance Institution of Finland; Swedish Heart-Lung Foundation; Swedish Diabetes Association; Swedish Research Council; City of Malmo; Pahlsson Foundation; Affymetrix Inc; Robert Dawson Evans Endowment; Doris Duke Charitable Foundation; Centre de Recherche Medicale de l'Universite de Sherbrooke (CRMUS); Canadian Institute of Health Research (CHIR); Erasmus Medical Center; Erasmus University; Kuopio University Hospital; Tampere University Hospital; Turku University Hospital; Juho Vainio Foundation; Paavo Nurmi Foundation; Finnish Foundation of Cardiovascular Research; Finnish Cultural Foundation; Tampere Tuberculosis Foundation; Emil Aaltonen Foundation; Novo Nordisk; Umea University; Heart Foundation of Northern Sweden, Region Shine; Cancer Research UK FX The following agencies contributed support: National Institutes of Health; NIH Roadmap for Medical Research; National Institute of Diabetes and Digestive and Kidney Diseases; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Neurological Disorders and Stroke; National Institute on Aging; National Center of Advancing Translational Technologies; US Department of Agriculture; Medical Research Council UK; Wellcome Trust; Cancer Research UK; Italian Ministry of Health; Netherlands Organisation of Scientific Research NWO Investments; The Research Institute for Diseases in the Elderly (014-93-015; RIDE2); The Netherlands Genomics Initiative (NGI)/Netherlands Consortium for Healthy Aging (NCHA), Rotterdam; Netherlands Organization for the Health Research and Development; The Research Institute for Diseases in the Elderly (RIDE); The Ministry of Education, Culture, and Science; The Ministry for Health, Welfare, and Sports; The European Commission and the Municipality of Rotterdam; Academy of Finland; Social Insurance Institution of Finland; Swedish Heart-Lung Foundation; Swedish Diabetes Association; Swedish Research Council; City of Malmo; Pahlsson Foundation; Affymetrix Inc; Robert Dawson Evans Endowment; Doris Duke Charitable Foundation; Centre de Recherche Medicale de l'Universite de Sherbrooke (CRMUS) and a Canadian Institute of Health Research (CHIR); Erasmus Medical Center and Erasmus University; Kuopio, Tampere and Turku University Hospital medical funds; Juho Vainio Foundation; Paavo Nurmi Foundation; Finnish Foundation of Cardiovascular Research and Finnish Cultural Foundation; Tampere Tuberculosis Foundation and Emil Aaltonen Foundation; Novo Nordisk; an Umea University Career Development Award; the Heart Foundation of Northern Sweden, Region Shine. NR 49 TC 48 Z9 49 U1 0 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUN PY 2013 VL 97 IS 6 BP 1395 EP 1402 DI 10.3945/ajcn.112.052183 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 150DX UT WOS:000319371500030 PM 23636237 ER PT J AU Ducey, TF Hunt, PG AF Ducey, Thomas F. Hunt, Patrick G. TI Microbial community analysis of swine wastewater anaerobic lagoons by next-generation DNA sequencing SO ANAEROBE LA English DT Article DE Anaerobic lagoon; Swine wastewater; Pyrosequencing; Microbial communities ID MANURE STORAGE PITS; GUT MICROBIOTA; SP NOV.; BACTERIA; DENITRIFICATION; POPULATIONS; INDICATORS; NUTRIENTS; EFFLUENT; SLURRY AB Anaerobic lagoons are a standard practice for the treatment of swine wastewater. This practice relies heavily on microbiological processes to reduce concentrated organic material and nutrients. Despite this reliance on microbiological processes, research has only recently begun to identify and enumerate the myriad and complex interactions that occur in this microbial ecosystem. To further this line of study, we utilized a next-generation sequencing (NGS) technology to gain a deeper insight into the microbial communities along the water column of four anaerobic swine wastewater lagoons. Analysis of roughly one million 16S rDNA sequences revealed a predominance of operational taxonomic units (OTUs) classified as belonging to the phyla Firmicutes (54.1%) and Proteobacteria (15.8%). At the family level, 33 bacterial families were found in all 12 lagoon sites and accounted for between 30% and 50% of each lagoon's OTUs. Analysis by nonmetric multidimensional scaling (NMS) revealed that TKN, COD, ORP, TSS, and DO were the major environmental variables in affecting microbial community structure. Overall, 839 individual genera were classified, with 223 found in all four lagoons. An additional 321 genera were identified in sole lagoons. The top 25 genera accounted for approximately 20% of the OTUs identified in the study, and the low abundances of most of the genera suggests that most OTUs are present at low levels. Overall, these results demonstrate that anaerobic lagoons have distinct microbial communities which are strongly controlled by the environmental conditions present in each individual lagoon. Published by Elsevier Ltd. C1 [Ducey, Thomas F.; Hunt, Patrick G.] ARS, USDA, CPSWPRC, Florence, SC 29501 USA. RP Ducey, TF (reprint author), ARS, USDA, CPSWPRC, 2611 West Lucas St, Florence, SC 29501 USA. EM thomas.ducey@ars.usda.gov OI Ducey, Thomas/0000-0001-8199-0584 NR 54 TC 6 Z9 7 U1 5 U2 69 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1075-9964 J9 ANAEROBE JI Anaerobe PD JUN PY 2013 VL 21 BP 50 EP 57 DI 10.1016/j.anaerobe.2013.03.005 PG 8 WC Microbiology SC Microbiology GA 152RM UT WOS:000319548800009 PM 23538056 ER PT J AU Shiro, S Matsuura, S Saiki, R Sigua, GC Yamamoto, A Umehara, Y Hayashi, M Saeki, Y AF Shiro, Sokichi Matsuura, Syota Saiki, Rina Sigua, Gilbert C. Yamamoto, Akihiro Umehara, Yosuke Hayashi, Masaki Saeki, Yuichi TI Genetic Diversity and Geographical Distribution of Indigenous Soybean-Nodulating Bradyrhizobia in the United States SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CONDITIONING INEFFECTIVE NODULATION; TRANSCRIBED SPACER REGION; RJ-GENOTYPE SOYBEANS; RHIZOBIUM-JAPONICUM; SP-NOV; SEQUENCE-ANALYSIS; GEN-NOV; RESISTANCE; FREDII; DIFFERENTIATION AB We investigated the relationship between the genetic diversity of indigenous soybean-nodulating bradyrhizobia and their geographical distribution in the United States using nine soil isolates from eight states. The bradyrhizobia were inoculated on three soybean Rj genotypes (non-Rj, Rj(2)Rj(3), and Rj(4)). We analyzed their genetic diversity and community structure by means of restriction fragment length polymorphisms of PCR amplicons to target the 16S-23S rRNA gene internal transcribed spacer region, using 11 USDA Bradyrhizobium strains as reference strains. We also performed diversity analysis, multidimensional scaling analysis based on the Bray-Curtis index, and polar ordination analysis to describe the structure and geographical distribution of the soybean-nodulating bradyrhizobial community. The major clusters were Bradyrhizobium japonicum Bj123, in the northern United States, and Bradyrhizobium elkanii, in the middle to southern regions. Dominance of bradyrhizobia in a community was generally larger for the cluster belonging to B. elkanii than for the cluster belonging to B. japonicum. The indigenous American soybean-nodulating bradyrhizobial community structure was strongly correlated with latitude. Our results suggest that this community varies geographically. C1 [Shiro, Sokichi] Miyazaki Univ, Interdisciplinary Grad Sch Agr & Engn, Miyazaki, Japan. [Matsuura, Syota; Saiki, Rina; Yamamoto, Akihiro; Saeki, Yuichi] Miyazaki Univ, Fac Agr, Dept Biochem & Appl Biosci, Miyazaki 88921, Japan. [Sigua, Gilbert C.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. [Umehara, Yosuke; Hayashi, Masaki] Natl Inst Agrobiol Sci, Ibaraki, Japan. RP Saeki, Y (reprint author), Miyazaki Univ, Fac Agr, Dept Biochem & Appl Biosci, Miyazaki 88921, Japan. EM yt-saeki@cc.miyazaki-u.ac.jp FU Japan Society for the Promotion of Science KAKENHI grant [22580068]; Ministry of Agriculture, Forestry and Fisheries of Japan FX This study was supported mainly by a Japan Society for the Promotion of Science KAKENHI grant (Grants-in-Aid for Scientific Research [C], no. 22580068) and partially by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation SOY-2001). NR 51 TC 15 Z9 15 U1 2 U2 25 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2013 VL 79 IS 12 BP 3610 EP 3618 DI 10.1128/AEM.00236-13 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 152CO UT WOS:000319507700009 PM 23563944 ER PT J AU Levine, UY Looft, T Allen, HK Stanton, TB AF Levine, Uri Y. Looft, Torey Allen, Heather K. Stanton, Thad B. TI Butyrate-Producing Bacteria, Including Mucin Degraders, from the Swine Intestinal Tract SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID MEGASPHAERA-ELSDENII STRAINS; ACIDAMINOCOCCUS-FERMENTANS; ANAEROBIC-BACTERIA; COA-TRANSFERASE; CECAL INFUSION; HUMAN FECES; COMBINATIONS; METABOLISM; PIGLETS; COLON AB To identify bacteria with potential for influencing gut health, 980 anaerobes were cultured from the swine intestinal tract and analyzed for butyrate production. Fifteen isolates in the order Clostridiales produced butyrate and had butyryl coenzyme A (CoA):acetate CoA transferase activity. Three of the isolates grew on mucin, suggesting an intimate association with host intestinal mucosa. C1 [Levine, Uri Y.; Looft, Torey; Allen, Heather K.; Stanton, Thad B.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Stanton, TB (reprint author), ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM Thad.Stanton@ars.usda.gov NR 29 TC 16 Z9 16 U1 2 U2 27 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2013 VL 79 IS 12 BP 3879 EP 3881 DI 10.1128/AEM.00589-13 PG 3 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 152CO UT WOS:000319507700043 PM 23584773 ER PT J AU Parejko, JA Mavrodi, DV Mavrodi, OV Weller, DM Thomashow, LS AF Parejko, James A. Mavrodi, Dmitri V. Mavrodi, Olga V. Weller, David M. Thomashow, Linda S. TI Taxonomy and Distribution of Phenazine-Producing Pseudomonas spp. in the Dryland Agroecosystem of the Inland Pacific Northwest, United States SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; PHENAZINE-1-CARBOXYLIC ACID; FLUORESCENT PSEUDOMONAS; PHYLOGENETIC TREES; RHIZOSPHERE; DIVERSITY; BACTERIA; BIOSYNTHESIS; POPULATIONS; WASHINGTON AB We investigated the taxonomic placement of phenazine-producing fluorescent Pseudomonas spp. in the Inland Pacific Northwest region of the United States. Five distinct species were identified, two of which were provisionally considered to be new. Agroclimatic zone and soil silt content affected the species diversity across the region. C1 [Parejko, James A.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Mavrodi, Dmitri V.; Mavrodi, Olga V.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Weller, David M.; Thomashow, Linda S.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. RP Thomashow, LS (reprint author), ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. EM thomashow@wsu.edu RI Mavrodi, Dmitri/J-2745-2013 FU National Institute of General Medical Sciences [T32GM083864] FX J.A.P. was supported in part by award T32GM083864 from the National Institute of General Medical Sciences. NR 22 TC 3 Z9 3 U1 1 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUN PY 2013 VL 79 IS 12 BP 3887 EP 3891 DI 10.1128/AEM.03945-12 PG 5 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 152CO UT WOS:000319507700045 PM 23584779 ER PT J AU Zolla, G Badri, DV Bakker, MG Manter, DK Viyanco, JM AF Zolla, Gaston Badri, Dayakar V. Bakker, Matthew G. Manter, Daniel K. Viyanco, Jorge M. TI Soil microbiomes vary in their ability to confer drought tolerance to Arabidopsis SO APPLIED SOIL ECOLOGY LA English DT Article DE Drought; Sympatric; Soil microbiome; Arabidopsis; Pyrosequencing ID GROWTH-PROMOTING RHIZOBACTERIA; ABIOTIC STRESS RESPONSES; BACTERIAL ACC DEAMINASE; WATER-USE EFFICIENCY; PLANT-GROWTH; GENE-EXPRESSION; TRANSCRIPTION FACTOR; ATACAMA DESERT; TOMATO PLANTS; THALIANA AB Drought is a major constraint on agricultural production. Crop genetic improvement for drought tolerance has received much attention and there is ample information about the ability of specific soil microbes to influence drought tolerance in plants. However, in nature, plants interact simultaneously with an array of beneficial, benign and pathogenic microbes. There is a need to understand the cumulative effect of these multiple interactions on a plant's ability to overcome abiotic stresses such as drought. The objective of this research was to investigate the potential of whole soil microbiomes to help Arabidopsis thaliana plants deal with drought stress under in vivo conditions. A sympatric microbiome (i.e., having a history of exposure to Arabidopsis at a natural site) significantly increased plant biomass under drought conditions, but caused earlier death rates as a consequence of drought; whereas, the two non-sympatric soils did not influence Arabidopsis biomass. Consistent with this, we observed reduced expression levels for several Arabidopsis drought response marker genes (ATDI21, DREB1A, DREB2A, and NCED3) in the sympatric Arabidopsis soil treatment. Pyrosequencing analysis of the three soil microbiomes used in this study identified 84 bacterial OTUs (3% genetic distance) from 41 genera (Burkholderia, Phormidium, Bacillus, Aminobacter, Acidiphilum and among others) that were significantly higher in the sympatric Arabidopsis soil, as compared to the two non-sympatric soils. In conclusion, we have identified a robust set of Arabidopsis-associated microbes that when present in the soil can modify the plant's ability to sense abiotic stress and increase its biomass production. (C) 2013 Elsevier B.V. All rights reserved. C1 [Zolla, Gaston; Badri, Dayakar V.; Bakker, Matthew G.; Viyanco, Jorge M.] Colorado State Univ, Ctr Rhizosphere Biol, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. [Manter, Daniel K.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. RP Viyanco, JM (reprint author), Colorado State Univ, Ctr Rhizosphere Biol, 1173 Campus Delivery, Ft Collins, CO 80523 USA. EM dayakar@lamar.colostate.edu; j.vivanco@colostate.edu RI Bakker, Matthew/B-8610-2013 OI Bakker, Matthew/0000-0003-0345-0587 FU National Science Foundation [MCB-0950857]; USDA-ARS FX This research was supported by a grant from the National Science Foundation to JMV (MCB-0950857) and by a Cooperative Agreement with the USDA-ARS. NR 56 TC 20 Z9 21 U1 13 U2 96 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JUN PY 2013 VL 68 BP 1 EP 9 DI 10.1016/j.apsoil.2013.03.007 PG 9 WC Soil Science SC Agriculture GA 156WB UT WOS:000319853500001 ER PT J AU Yao, HB Hruska, Z Kincaid, R Brown, RL Bhatnagar, D Cleveland, TE AF Yao, Haibo Hruska, Zuzana Kincaid, Russell Brown, Robert L. Bhatnagar, Deepak Cleveland, Thomas E. TI Detecting maize inoculated with toxigenic and atoxigenic fungal strains with fluorescence hyperspectral imagery SO BIOSYSTEMS ENGINEERING LA English DT Article ID ASPERGILLUS-FLAVUS; UNITED-STATES; FECAL CONTAMINATION; SECTION FLAVI; AFLATOXIN; REFLECTANCE; BIOCONTROL; KERNELS AB Naturally occurring Aspergillus flavus strains can be either toxigenic or atoxigenic, indicating their ability to produce aflatoxin or not. The objective was to assess, with the use of a hyperspectral sensor, the difference in fluorescence emission between maize kernels inoculated with toxigenic and atoxigenic inoculums of A. flavus. Maize ears were inoculated with AF13, a toxigenic strain of A. flavus, and AF38, an atoxigenic strain of A. flavus, at dough stage of development and harvested 8 weeks after inoculation. After harvest, single kernels were divided into three groups prior to imaging: control, adjacent, and glowing. Both sides of the kernel, germ and endosperm, were imaged separately using a fluorescence hyperspectral imaging system. After imaging each single kernel was processed with affinity column fluorimetry to determine aflatoxin level. Results from discriminant analysis of the imaging data found that the classification accuracies of the three visually designated groups were not promising. The separation of maize kernels based on different fungal inoculums yielded better results. The best results were achieved with the germ side of the maize kernels. The kernels were later grouped into 'contaminated' and 'healthy' with 20 ppb and 100 ppb thresholds. The contaminated kernels all had longer peak wavelength than did the healthy ones. Results from the discriminant analysis classification indicated overall higher classification accuracy for the 100 ppb threshold on the germ side (94.4%). The germ side was also more useful at discriminating healthy from contaminated kernels for the 20 ppb threshold. (C) 2013 IAgrE. Published by Elsevier Ltd. All rights reserved. C1 [Yao, Haibo; Hruska, Zuzana; Kincaid, Russell] Mississippi State Univ, Geosyst Res Inst, Stennis Space Ctr, MS 39529 USA. [Brown, Robert L.; Bhatnagar, Deepak; Cleveland, Thomas E.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Yao, HB (reprint author), Mississippi State Univ, Geosyst Res Inst, Bldg 1021, Stennis Space Ctr, MS 39529 USA. EM haibo@gri.msstate.edu FU USDA [58-6435-9-425] FX Funding for this work was provided by the USDA cooperative agreement 58-6435-9-425. The authors would like to thank Dr. Zhiyuan Chen from Louisiana State University, Baton Rouge, LA, USA for help with producing the maize samples for this study. NR 26 TC 16 Z9 16 U1 3 U2 27 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 J9 BIOSYST ENG JI Biosyst. Eng. PD JUN PY 2013 VL 115 IS 2 BP 125 EP 135 DI 10.1016/j.biosystemseng.2013.03.006 PG 11 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA 153ZS UT WOS:000319641900002 ER PT J AU Arendt, WJ Qian, SS Mineard, KA AF Arendt, Wayne J. Qian, Song S. Mineard, Kelli A. TI Population decline of the Elfin-woods Warbler Setophaga angelae in eastern Puerto Rico SO BIRD CONSERVATION INTERNATIONAL LA English DT Article ID LUQUILLO MOUNTAINS; FOREST STRUCTURE; STATE FOREST; WOODLAND; GROWTH AB We estimated the population density of the globally threatened Elfin-woods Warbler Setophaga angelae within two forest types at different elevations in the Luquillo Experimental Forest in north-eastern Puerto Rico. Population densities ranged from 0.01 to 0.02 individuals/ha in elfin woodland and 0.06-0.26 individuals/ha in palo colorado forest in 2006, with average rates of decline since 1989 of 0.002-0.01 and 0.003-0.06 individuals/ha respectively. These estimates show a significant general declining trend from c.0.2 individuals/ha in 1989 in elfin woodland to c.0.02/ha in 2006, and from 1 to 0.2 in palo colorado forest. Although variation in estimated population density depended on the statistical method used, we document and discuss possible causes of an overall population decline from 1989 to 2006, lending support to previous initiatives to reclassify the species from the IUCN Red List category of "Vulnerable" to "Endangered". C1 [Arendt, Wayne J.] US Forest Serv, USDA, Int Inst Trop Forestry, Sabana Field Res Stn, Luquillo, PR 00773 USA. [Qian, Song S.; Mineard, Kelli A.] Duke Univ, Nicholas Sch Environm & Earth Sci, Durham, NC 27708 USA. RP Arendt, WJ (reprint author), US Forest Serv, USDA, Int Inst Trop Forestry, Sabana Field Res Stn, HC 2 Box 6205, Luquillo, PR 00773 USA. EM waynearendt@mac.com NR 39 TC 1 Z9 1 U1 2 U2 7 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0959-2709 J9 BIRD CONSERV INT JI Bird Conserv. Int. PD JUN PY 2013 VL 23 IS 2 BP 136 EP 146 DI 10.1017/S0959270913000166 PG 11 WC Ornithology SC Zoology GA 146WP UT WOS:000319126200004 ER PT J AU Mizushima, A Lu, RF AF Mizushima, Akira Lu, Renfu TI An image segmentation method for apple sorting and grading using support vector machine and Otsu's method SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Segmentation; Support vector machine; Otsu's method; Automatic thresholding; Apple sorting and grading ID VISION SYSTEM; COMPUTER VISION; CAMERAS; FRUIT; SIZE AB Segmentation is the first step in image analysis to subdivide an image into meaningful regions. It directly affects the subsequent image analysis outcomes. This paper reports on the development of an automatic adjustable algorithm for segmentation of color images, using linear support vector machine (SVM) and Otsu's thresholding method, for apple sorting and grading. The method automatically adjusts the classification hyperplane calculated by using linear SVM and requires minimum training and time. It also avoids the problems caused by variations in the lighting condition and/or the color of the fruit. To evaluate the robustness and accuracy of the proposed segmentation method, tests were conducted for 300 'Delicious' apples using three training samples with different color characteristics (i.e., orange, stripe, and dark red) and their combination. The segmentation error varied from 3% to 25% for the fixed SVM, while the adjustable SVM achieved consistent and accurate results for each training set, with the segmentation error of less than 2%. The proposed method provides an effective and robust segmentation means for sorting and grading apples in a multi-channel color space, and it can be easily adapted for other imaging-based agricultural applications. Published by Elsevier B.V. C1 [Mizushima, Akira; Lu, Renfu] Michigan State Univ, USDA, ARS, E Lansing, MI 48824 USA. RP Mizushima, A (reprint author), Michigan State Univ, USDA, ARS, 524 S Shaw Lane,Room 207, E Lansing, MI 48824 USA. EM Akira.Mizushima@ars.usda.gov FU Michigan Apple Committee under the Trust Fund Cooperative Agreement [58-3635-9-570] FX The research was supported in part by funds from Michigan Apple Committee under the Trust Fund Cooperative Agreement No. 58-3635-9-570 between the Michigan Apple Committee and the USDA Agricultural Research Service. NR 26 TC 25 Z9 32 U1 7 U2 67 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD JUN PY 2013 VL 94 BP 29 EP 37 DI 10.1016/j.compag.2013.02.009 PG 9 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 153ZJ UT WOS:000319641000004 ER PT J AU Schaefer, EJ AF Schaefer, Ernst J. TI introduction SO CURRENT OPINION IN LIPIDOLOGY LA English DT Editorial Material C1 [Schaefer, Ernst J.] Tufts Univ, Boston, MA 02111 USA. RP Schaefer, EJ (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Lipid Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM ernst.schaefer@tufts.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0957-9672 J9 CURR OPIN LIPIDOL JI Curr. Opin. Lipidology PD JUN PY 2013 VL 24 IS 3 BP 191 EP 191 DI 10.1097/MOL.0b013e328361243f PG 1 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Peripheral Vascular Disease SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Cardiovascular System & Cardiology GA 151FO UT WOS:000319446300001 PM 23652566 ER PT J AU Lamon-Fava, S AF Lamon-Fava, Stefania TI Statins and lipid metabolism: an update SO CURRENT OPINION IN LIPIDOLOGY LA English DT Review DE ABCA1; HDL; LDL; metabolism; statin ID LOW-DENSITY-LIPOPROTEIN; REVERSE CHOLESTEROL TRANSPORT; STEROL-REGULATORY ELEMENT; HETEROZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA; APOLIPOPROTEIN B-100 KINETICS; TYPE-2 DIABETES-MELLITUS; APOA-I KINETICS; A-I; ABCA1 EXPRESSION; REDUCTASE INHIBITORS AB Purpose of review The reduction in cardiovascular disease risk by statins is well established. This risk reduction has mostly been attributed to decreases in plasma LDL cholesterol and other pleiotropic effects of statins. Emerging evidence indicates that statins exert multiple effects on lipoprotein metabolism, including chylomicrons and HDLs. Recent findings Kinetic and in-vitro studies have documented that the effects of statins on the metabolism of different lipoproteins are for the most part the direct consequence of cholesterol biosynthesis inhibition and the subsequent change in transcription factors and cell signaling, regulating different aspects of lipoprotein metabolism. Differences in pharmacokinetics and pharmacodynamics among statins lead to diverse biological outcomes. Summary The current review summarizes recent experimental evidence highlighting the different effects of statins on cellular pathways regulating gene expression. Understanding the basic mechanisms by which different statins regulate lipoprotein metabolism will lead to improved strategies for the prevention and treatment of specific lipoprotein disorders. C1 [Lamon-Fava, Stefania] Tufts Univ, Lipid Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lamon-Fava, Stefania] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. RP Lamon-Fava, S (reprint author), Jean Mayer USDA HNRCA, Lipid Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM stefania.lamon-fava@tufts.edu NR 59 TC 9 Z9 10 U1 1 U2 24 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0957-9672 J9 CURR OPIN LIPIDOL JI Curr. Opin. Lipidology PD JUN PY 2013 VL 24 IS 3 BP 221 EP 226 DI 10.1097/MOL.0b013e3283613b8b PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Peripheral Vascular Disease SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Cardiovascular System & Cardiology GA 151FO UT WOS:000319446300006 PM 23571733 ER PT J AU Hesler, LS Chiozza, MV O'Neal, ME MacIntosh, GC Tilmon, KJ Chandrasena, DI Tinsley, NA Cianzio, SR Costamagna, AC Cullen, EM DiFonzo, CD Potter, BD Ragsdale, DW Steffey, K Koehler, KJ AF Hesler, Louis S. Chiozza, Mariana V. O'Neal, Matthew E. MacIntosh, Gustavo C. Tilmon, Kelley J. Chandrasena, Desmi I. Tinsley, Nicholas A. Cianzio, Silvia R. Costamagna, Alejandro C. Cullen, Eileen M. DiFonzo, Christina D. Potter, Bruce D. Ragsdale, David W. Steffey, Kevin Koehler, Kenneth J. TI Performance and prospects of Rag genes for management of soybean aphid SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Review DE Aphis glycines; host plant resistance; virulent biotypes; pest management; crop protection; yield penalty; Hemiptera; Aphididae; Fabaceae ID SINGLE DOMINANT GENE; HEMIPTERA APHIDIDAE; POPULATION-DYNAMICS; NORTH-AMERICA; PLANT-RESISTANCE; PATTERN-ANALYSIS; SEED TRAITS; YIELD; GLYCINES; INHERITANCE AB The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is an invasive insect pest of soybean [Glycine max (L.) Merr. (Fabaceae)] in North America, and it has led to extensive insecticide use in northern soybean-growing regions there. Host plant resistance is one potential alternative strategy for managing soybean aphid. Several Rag genes that show antibiosis and antixenosis to soybean aphid have been recently identified in soybean, and field-testing and commercial release of resistant soybean lines have followed. In this article, we review results of field tests with soybean lines containing Rag genes in North America, then present results from a coordinated regional test across several field sites in the north-central USA, and finally discuss prospects for use of Rag genes to manage soybean aphids. Field tests conducted independently at multiple sites showed that soybean aphid populations peaked in late summer on lines with Rag1 or Rag2 and reached economically injurious levels on susceptible lines, whereas lines with a pyramid of Rag1+Rag2 held soybean aphid populations below economic levels. In the regional test, aphid populations were generally suppressed by lines containing one of the Rag genes. Aphids reached putative economic levels on Rag1 lines for some site years, but yield loss was moderated, indicating that Rag1 may confer tolerance to soybean aphid in addition to antibiosis and antixenosis. Moreover, no yield penalty has been found for lines with Rag1, Rag2, or pyramids. Results suggest that use of aphid-resistant soybean lines with Rag genes may be viable for managing soybean aphids. However, virulent biotypes of soybean aphid were identified before release of aphid-resistant soybean, and thus a strategy for optimal deployment of aphid-resistant soybean is needed to ensure sustainability of this technology. C1 [Hesler, Louis S.] ARS, USDA, NCARL, Brookings, SD 57006 USA. [Chiozza, Mariana V.; O'Neal, Matthew E.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [MacIntosh, Gustavo C.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Tilmon, Kelley J.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Chandrasena, Desmi I.; DiFonzo, Christina D.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Tinsley, Nicholas A.; Steffey, Kevin] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Cianzio, Silvia R.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Costamagna, Alejandro C.; Ragsdale, David W.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Cullen, Eileen M.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Potter, Bruce D.] Univ Minnesota, Southwest Res & Outreach Ctr, Lamberton, MN 56152 USA. [Koehler, Kenneth J.] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. RP Hesler, LS (reprint author), ARS, USDA, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov RI Costamagna, Alejandro/B-1750-2009; OI MacIntosh, Gustavo/0000-0003-1350-1229 FU North Central Soybean Research program; USDA-ARS CRIS Project [5447-21220-005-00D] FX We thank the North Central Soybean Research program for funding the regional project. Brian Diers, John Reese, Roy Scott, Dechun Wang, and Marci Green developed soybean lines and provided seeds for the regional experiment. Gregory Gebhart, Peter Lundeen, Felicitas Avendano no, Kevin Johnson, Ron Estes, and Joshua Heeren collaborated on the regional experiment. Brian Diers, Erin Hodgson, Eric Beckendorf, and Lauren Hesler reviewed earlier drafts of this paper. The study described in this article was funded partially by USDA-ARS CRIS Project 5447-21220-005-00D. NR 88 TC 23 Z9 24 U1 4 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUN PY 2013 VL 147 IS 3 BP 201 EP 216 DI 10.1111/eea.12073 PG 16 WC Entomology SC Entomology GA 151OQ UT WOS:000319470300001 ER PT J AU Barker, S Covello, JM Bouchard, D Wolters, W Fast, M Bricknell, I AF Barker, S. Covello, J. M. Bouchard, D. Wolters, W. Fast, M. Bricknell, I. TI Lice and isav: Are 'lousy' salmon more susceptible? SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract C1 [Barker, S.; Bouchard, D.; Bricknell, I.] Univ Maine, Sch Marine Sci, Orono, ME USA. [Covello, J. M.; Fast, M.] Univ Prince Edward Isl, Atlantic Vet Coll, Charlottetown, PE C1A 4P3, Canada. [Wolters, W.] ARS, USDA, Natl Cold Water Marine Aquaculture Ctr, Franklin, ME USA. EM sarah.barker@umit.maine.edu; jcovello@upei.ca NR 0 TC 1 Z9 1 U1 1 U2 19 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2013 VL 34 IS 6 BP 1637 EP 1638 PG 2 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA 154BI UT WOS:000319646100036 ER PT J AU Hansen, JD Ray, K Woodson, JC Soto, E Welch, TJ AF Hansen, J. D. Ray, K. Woodson, J. C. Soto, E. Welch, T. J. TI Disruption of the Francisella noatunensis PDPA gene results in virulence attenuation SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract C1 [Hansen, J. D.; Ray, K.; Woodson, J. C.] US Geol Survey, Western Fisheries Res Ctr, Seattle, WA USA. [Hansen, J. D.] Univ Washington, Interdisciplinary Program Pathobiol, Seattle, WA 98195 USA. [Welch, T. J.] USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV USA. EM jhansen@usgs.gov NR 0 TC 1 Z9 1 U1 0 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2013 VL 34 IS 6 BP 1655 EP 1655 PG 1 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA 154BI UT WOS:000319646100089 ER PT J AU Quiniou, SMA Boudinot, P Bengten, E AF Quiniou, S. M. A. Boudinot, P. Bengten, E. TI Comprehensive survey and genomic characterization of Toll-Like Receptors (TLRs) in channel catfish, Ictalurus punctatus: Identification of novel fish TLRs SO FISH & SHELLFISH IMMUNOLOGY LA English DT Meeting Abstract C1 [Quiniou, S. M. A.] USDA ARS, Catfish Genet Res Unit, Stoneville, MS 38776 USA. [Boudinot, P.] INRA, Mol Virol & Immunol, F-78350 Jouy En Josas, France. [Bengten, E.] Univ Mississippi, Med Ctr, Dept Microbiol, Jackson, MS 39216 USA. EM sylvie.quiniou@ars.usda.gov RI boudinot, pierre/L-4246-2016 NR 0 TC 0 Z9 0 U1 2 U2 8 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD JUN PY 2013 VL 34 IS 6 BP 1731 EP 1731 PG 1 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA 154BI UT WOS:000319646100314 ER PT J AU Anderson, OD Huo, NX Gu, YQ AF Anderson, Olin D. Huo, Naxin Gu, Yong Q. TI The gene space in wheat: the complete gamma-gliadin gene family from the wheat cultivar Chinese Spring SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Wheat; Gliadins; Gene family; Gene activity; Gene evolution ID TRITICUM-AESTIVUM L.; EXPRESSION; PROTEINS; BREAD; ORGANIZATION; PSEUDOGENES; RESOURCE; LOCI AB The complete set of unique gamma-gliadin genes is described for the wheat cultivar Chinese Spring using a combination of expressed sequence tag (EST) and Roche 454 DNA sequences. Assemblies of Chinese Spring ESTs yielded 11 different gamma-gliadin gene sequences. Two of the sequences encode identical polypeptides and are assumed to be the result of a recent gene duplication. One gene has a 3' coding mutation that changes the reading frame in the final eight codons. A second assembly of Chinese Spring gamma-gliadin sequences was generated using Roche 454 total genomic DNA sequences. The 454 assembly confirmed the same 11 active genes as the EST assembly plus two pseudogenes not represented by ESTs. These 13 gamma-gliadin sequences represent the complete unique set of gamma-gliadin genes for cv Chinese Spring, although not ruled out are additional genes that are exact duplications of these 13 genes. A comparison with the ESTs of two other hexaploid cultivars (Butte 86 and Recital) finds that the most active genes are present in all three cultivars, with exceptions likely due to too few ESTs for detection in Butte 86 and Recital. A comparison of the numbers of ESTs per gene indicates differential levels of expression within the gamma-gliadin gene family. Genome assignments were made for 6 of the 13 Chinese Spring gamma-gliadin genes, i.e., one assignment from a match to two gamma-gliadin genes found within a tetraploid wheat A genome BAC and four genes that match four distinct gamma-gliadin sequences assembled from Roche 454 sequences from Aegilops tauschii, the hexaploid wheat D-genome ancestor. C1 [Anderson, Olin D.; Huo, Naxin; Gu, Yong Q.] ARS, Genom & Gene Discovery Res Unit, Western Reg Res Unit, USDA, Albany, CA 94710 USA. [Huo, Naxin] Univ Calif Davis, Davis, CA 95616 USA. RP Anderson, OD (reprint author), ARS, Genom & Gene Discovery Res Unit, Western Reg Res Unit, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM olin.anderson@ars.usda.gov FU USDA-ARS [5325-21000-015]; National Science Foundation [0701916] FX The research was supported by USDA-ARS project 5325-21000-015 and National Science Foundation grant 0701916. NR 22 TC 11 Z9 11 U1 2 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD JUN PY 2013 VL 13 IS 2 BP 261 EP 273 DI 10.1007/s10142-013-0321-8 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 150ZV UT WOS:000319431300010 PM 23564033 ER PT J AU Shelly, TE Edu, J Nishimoto, J AF Shelly, T. E. Edu, J. Nishimoto, J. TI Chilling and flight ability and mating competitiveness of sterile males of the Mediterranean fruit fly SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Ceratitis capitata; insect release; quality control; Sterile Insect Technique ID CERATITIS-CAPITATA DIPTERA; MASS-REARED MALES; INSECT TECHNIQUE; FLIES DIPTERA; TEPHRITIDAE; COURTSHIP; PERFORMANCE; BEHAVIOR; RELEASE; DENSITY AB The Sterile Insect Technique (SIT) is widely used to suppress or eradicate infestations of the Mediterranean fruit fly, Ceratitis capitata (Wied.). In large-scale programs, sterile males are chilled (4 degrees C) to allow their transfer and storage in the aircraft used for the releases. Sterile males may remain chilled for as long as 3h prior to release. Here, we describe the results of experiments that assessed the effect of chilling on flight ability and mating competitiveness of sterile male Mediterranean fruit flies held under conditions of low (plastic buckets) or high (emergence tower trays) density. Males from both densities were subject to 0 (no chill), 1, or 3h of chilling at 3days of age. Chill treatment had no effect on flight ability for males held at low density. However, for males held at high density, chilling for 1 or 3h significantly reduced flight ability below that observed for the no chill treatment. Consistent with the flight data, chill treatment had no effect on the mating success of males held at low density. However, among males held at high density, 3h of chilling significantly reduced mating success below levels observed for unchilled males or males chilled for 1h only in trials conducted 1day after the cold treatment. An auxiliary experiment revealed that this reduction in mating performance was temporary: in mating trials conducted 3days after 3h of chilling, sterile males derived from tower trays had similar mating success as unchilled males. Implications of these findings for Mediterranean fruit fly SIT are discussed. C1 [Shelly, T. E.; Edu, J.; Nishimoto, J.] APHIS USDA, Waimanalo, HI USA. RP Shelly, TE (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 30 TC 5 Z9 5 U1 1 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 11 EP 18 DI 10.1111/j.1439-0418.2010.01532.x PG 8 WC Entomology SC Entomology GA 153DW UT WOS:000319582600003 ER PT J AU Segura, DF Utges, ME Liendo, MC Rodriguez, MF Devescovi, F Vera, MT Teal, PEA Cladera, JL AF Segura, D. F. Utges, M. E. Liendo, M. C. Rodriguez, M. F. Devescovi, F. Vera, M. T. Teal, P. E. A. Cladera, J. L. TI Methoprene treatment reduces the pre-copulatory period in Anastrepha fraterculus (Diptera: Tephritidae) sterile males SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE juvenile hormone; sexual maturation; South American fruit fly; sterile insect technique ID CERATITIS-CAPITATA DIPTERA; MEDITERRANEAN FRUIT-FLIES; JUVENILE-HORMONE; FLY DIPTERA; INSECT TECHNIQUE; BEHAVIOR; POPULATIONS; COMPATIBILITY; REPRODUCTION; MATURATION AB Anastrepha fraterculus is a major fruit pest in South America. Ongoing studies support the implementation of the sterile insect technique (SIT) against this pest. Sexual readiness of sterile males is a key point for SIT application. The pre-copulatory period of A.fraterculus males has not been reported before, but it is expected to last several days. An acceleration of sexual maturation was achieved in other Anastrepha species after topical applications of juvenile hormone analogues, like methoprene. Here, we studied the effect of methoprene on male sexual maturation, mating duration and sperm transfer in A.fraterculus as well as the impact of acetone (methoprene solvent) on survival. We also explored a method to deliver methoprene massively. Pheromone-calling and mating ability were evaluated daily from adult emergence, and used as indicators of sexual maturity. Anastrepha fraterculus males showed a long pre-copulatory period (7days approximately), as other Anastrepha species. This process was accelerated after methoprene treatment (2.5g/l), both in non-irradiated and irradiated males which matured 2-3days earlier. Mating duration for methoprene-treated males was longer than for mature untreated males, however, no differences in sperm transfer were detected. Survival was not affected by acetone. Dipping pupae in methoprene allowed emerging males to mature as fast as those receiving topical application as adults. Dipping of pupae is a promising method to deliver massively methoprene and should be further investigated. C1 [Segura, D. F.; Liendo, M. C.; Rodriguez, M. F.; Devescovi, F.; Cladera, J. L.] INTA Castelar, Inst Genet EA Favret, CNIA, Buenos Aires, DF, Argentina. [Segura, D. F.; Utges, M. E.; Liendo, M. C.; Vera, M. T.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Utges, M. E.] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Buenos Aires, DF, Argentina. [Vera, M. T.] Estn Expt Agroind Obispo Colombres, San Miguel De Tucuman, Argentina. [Teal, P. E. A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. RP Segura, DF (reprint author), INTA Castelar, Inst Genet EA Favret, CNIA, B1712DIA, Castelar, Argentina. EM dsegura@cnia.inta.gov.ar OI Segura, Diego/0000-0001-7714-6559; Utges, Maria Eugenia/0000-0002-8577-0359 FU FAO/IAEA; Ministerio de Ciencia, Tecnologia e Innovacion Productiva of Argentina [12852]; Ministerio de Ciencia, Tecnologia e Innovacion Productiva of Argentina (FONCYT PICT) [148] FX We thank Fabian H. Milla, Andrea Oviedo, Solana Abraham and Guillermo Bachmann for their collaboration to obtain the flies needed for these experiments, and Veronica Yusef and the Comision Nacional de Energia Atomica for irradiated flies. We also thank Rui Pereira for his valuable contribution in the experimental design of dipping experiments and his comments on a previous version of this manuscript. These studies were funded by the FAO/IAEA and the Ministerio de Ciencia, Tecnologia e Innovacion Productiva of Argentina (through the Research Contract 12852 to JLC and the FONCYT PICT 148 to DFS, respectively). NR 47 TC 16 Z9 16 U1 0 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 19 EP 29 DI 10.1111/j.1439-0418.2010.01534.x PG 11 WC Entomology SC Entomology GA 153DW UT WOS:000319582600004 ER PT J AU McInnis, DO Paranhos, BJ Shelly, TE AF McInnis, D. O. Paranhos, B. J. Shelly, T. E. TI Survival of sterile male Mediterranean fruit flies in large field cages after release at different ages SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Ceratitis capitata; field survival; immature; mating age; sterile insect technique ID CERATITIS-CAPITATA DIPTERA; FLY DIPTERA; MATING PROPENSITY; INSECT TECHNIQUE; TEPHRITIDAE; HAWAII; METHOPRENE; COURTSHIP; SUCCESS; LEKS AB In Mediterranean fruit fly (Ceratitis capitata) sterile insect technique (SIT) programs worldwide, sterile flies are usually released at 2-3days of age. However, they usually do not reach full sexual maturity until ca. 5days of age. We tested whether holding sterile males longer at a fly emergence and release facility, until they were 5days old, might result in significantly more flies surviving to reach sexual maturity in the field. In large field cages in Hawaii, we released sterile Mediterranean fruit fly males 2 or 5days old under conditions where food and water were provided, or not provided. Flies were released 2days after peak emergence in one field cage, while they were released 5days after peak emergence in a second field cage. The numbers of flies flying out and remaining (dead, dying or non-flying) in the holding boxes were recorded on the day of fly release. At 5 and 8days of fly age, the size of the male fly populations were estimated using trimedlure-baited traps placed into each of the two field cages for a 30-min period when the numbers of flies trapped were compared. Following six tests (three replications each with and without water and sugar provided), the differences in fly captures (i.e. survival) between 2days vs. 5days old releases were highly significant. With food and water provided, several times as many flies from the 5-day-old release field cage were captured at 5 and 8days of age compared to the 2-day-old release field cage. These differences were magnified under conditions of no food and water provided. Holding Mediterranean fruit flies longer prior to release, requires more holding space and food, but will lead to significantly greater numbers of sexually mature flies in the field. C1 [McInnis, D. O.] USDA ARS PBARC, Honolulu, HI USA. [Paranhos, B. J.] EMBRAPA, Semiarido Entomol Lab, Petr, Brazil. [Shelly, T. E.] USDA APHIS CPHST, Waimanalo, HI USA. RP McInnis, DO (reprint author), 1216 Manu Aloha St, Kailua, HI 96734 USA. EM moscamed@aol.com NR 20 TC 5 Z9 5 U1 1 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 43 EP 48 DI 10.1111/j.1439-0418.2010.01567.x PG 6 WC Entomology SC Entomology GA 153DW UT WOS:000319582600007 ER PT J AU Sookar, P Haq, I Jessup, A McInnis, D Franz, G Wornoayporn, V Permalloo, S AF Sookar, P. Haq, I. Jessup, A. McInnis, D. Franz, G. Wornoayporn, V. Permalloo, S. TI Mating compatibility among Bactrocera cucurbitae (Diptera: Tephritidae) populations from three different origins SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE melon fly; genetic sexing strain; sterile insect technique; sexual isolation ID MEDITERRANEAN FRUIT-FLY; MEDFLY DIPTERA; MELON FLY; ERADICATION; MALES AB Distinct host ranges of the melon fly, Bactrocera cucurbitae (Coquillett), have been reported among different island populations, suggesting significant genetic divergence. Thus, for the application of the sterile insect technique (SIT), it is important to ensure that released flies are sexually fully compatible with each other and with the laboratory strains. Mating tests among the following strains of the melon fly, B. cucurbitae: Mauritius laboratory-adapted (35 generations), Seychelles laboratory-adapted (24 generations), and Hawaii genetic sexing strain (90 generations), were conducted in field cages at the FAO/IAEA Agriculture and Biotechnology Laboratories in Seibersdorf, Austria during the months of August/September 2009. The genetic sexing strain, developed in Hawaii in 2001, allows separation of females and males on the basis of pupal colour. Two separate series of trials were run simultaneously. In the first, melon fly females from Mauritius were the target strain and the competing males were from Mauritius, Seychelles, and Hawaii (GSS). In the second trial, melon fly females from the Seychelles selected among competing males from the same three populations. Sexual activity was similar among the melon fly populations and no significant non-random, assortative mating was observed. Therefore, it is suggested that melon flies from Mauritius, Seychelles and the Hawaii are compatible, at least under semi-natural conditions. C1 [Sookar, P.; Permalloo, S.] Minist Agro Ind Food Prod & Secur, Agr Serv, Div Entomol, Reduit, Mauritius. [Haq, I.; Jessup, A.; Franz, G.; Wornoayporn, V.] FAO IAEA Agr & Biotechnol Lab, Insect Pest Control Lab, Seibersdorf, Austria. [McInnis, D.] ARS, USDA, Pacific Basin Area Res Ctr, Honolulu, HI USA. RP Sookar, P (reprint author), Minist Agro Ind Food Prod & Secur, Agr Serv, Div Entomol, Reduit, Mauritius. EM psookar@mail.gov.mu RI Jessup, Andrew/B-2510-2016; OI Jessup, Andrew/0000-0003-2318-7835; , Ihsan/0000-0001-5879-7698; Jessup, Andrew/0000-0002-0479-7489 FU FAO/IAEA [12859] FX This study was funded by the FAO/IAEA through the research contract No 12859. We would like to thank Mr A. Islam and Mr T. Dammalage for their support in the laboratory, as well as Teresa Vera (Estacion Experimental Agroindustrial Obispo Colombres, Tucuman, Argentina), Jorge Hendrichs and Rui Pereira (both from, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria) for critical reviews of an earlier version of this manuscript. NR 29 TC 6 Z9 6 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 69 EP 74 DI 10.1111/j.1439-0418.2010.01576.x PG 6 WC Entomology SC Entomology GA 153DW UT WOS:000319582600010 ER PT J AU Paranhos, BJ McInnis, D Morelli, R Castro, RM Garziera, L Paranhos, LG Costa, K Gava, C Costa, MLZ Walder, JMM AF Paranhos, B. J. McInnis, D. Morelli, R. Castro, R. M. Garziera, L. Paranhos, L. G. Costa, K. Gava, C. Costa, M. L. Z. Walder, J. M. M. TI Optimum dose of ginger root oil to treat sterile Mediterranean fruit fly males (Diptera: Tephritidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Ceratitis capitata; aromatherapy; medfly; sterile insect technique ID CERATITIS-CAPITATA DIPTERA; ENHANCES MATING SUCCESS; GENETIC SEXING STRAIN; MASS-REARED MALES; FLIES DIPTERA; COMPETITIVENESS; EXPOSURE; FIELD; AROMATHERAPY; PROGRAMS AB The sterile insect technique (SIT) is widely used as part of an integrated approach to reduce field populations of the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Aromatherapy based on exposure to ginger root oil (GRO) volatiles is known as a method to significantly improve the sexual performance of sterile medfly males, and is being used in many mass-rearing facilities around the world. However, the optimum dose of GRO is not well defined. This work evaluated in laboratory cages four different doses of GRO and a control (0, 0.1, 0.25, 0.5 and 0.75ml/m3), and two different methods to hold flies prior to release (paper bags and plastic cages). The objective was to find the lowest dose that provides optimal improvement in mating performance of sterile males when competing with wild males for wild females and optimal reduction in fertility of wild females. Egg hatch, copula duration, the Relative Sterility Index (RSI), and a Competitiveness (C) value (based on RSI) were calculated for each treatment to assess male sexual performance and induction of sterility. The method used to hold flies did not influence the aromatherapy effect. The mean time spent by wild females in copula with wild males was significantly longer than with sterile males for all treatments, except when sterile males were treated with 0.1ml of GRO/m3. Amongst all doses studied, it is recommended to apply the lowest dose, 0.1ml of GRO/m3, since at this dose both the RSI and induced sterility reached the highest levels, and were not statistically different from the 0.25 and 0.5 GRO/m3 doses. Indeed, this dose showed a very significant improvement in the mating competitiveness of sterile males aromatically treated with GRO. C1 [Paranhos, B. J.; Castro, R. M.; Garziera, L.; Paranhos, L. G.; Gava, C.] Embrapa Semiarido, Entomol Lab, BR-56302970 Petrolina, PE, Brazil. [McInnis, D.] ARS, USDA, Pacific Basin Agr Res Ctr, Honolulu, HI USA. [Morelli, R.] Univ Sao Paulo, ESALQ, Dept Entomol & Acarol, Piracicaba, SP, Brazil. [Costa, K.; Costa, M. L. Z.; Walder, J. M. M.] Univ Sao Paulo, CENA, Lab Food Irradiat & Radioentomol, Piracicaba, SP, Brazil. RP Paranhos, BJ (reprint author), Embrapa Semiarido, BR 428,Km 152,CP 23, BR-56302970 Petrolina, PE, Brazil. EM bjordao@cpatsa.embrapa.br FU Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA) [13495] FX We would like to thank Todd Shelly for comments on an earlier version of the manuscript and also Biofabrica Moscamed Brasil for providing the sterile males for these experiments. This research was supported by the Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA) through research contract No 13495. NR 38 TC 6 Z9 6 U1 0 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 83 EP 90 DI 10.1111/j.1439-0418.2010.01595.x PG 8 WC Entomology SC Entomology GA 153DW UT WOS:000319582600012 ER PT J AU Teal, PEA Pereira, R Segura, DF Haq, I Gomez-Simuta, Y Robinson, AS Hendrichs, J AF Teal, P. E. A. Pereira, R. Segura, D. F. Haq, I. Gomez-Simuta, Y. Robinson, A. S. Hendrichs, J. TI Methoprene and protein supplements accelerate reproductive development and improve mating success of male tephritid flies SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE adult diet; juvenile hormone; methoprene; sexual maturity; sexual performance ID CARIBBEAN FRUIT-FLIES; ANASTREPHA-SUSPENSA DIPTERA; IN-FIELD CAGES; FLY DIPTERA; SEXUAL PERFORMANCE; DIETARY-PROTEIN; LOEW DIPTERA; PRECOCENE-II; FOOD; COMPETITIVENESS AB We have been studying the behavioural and physiological mechanisms associated with coordination of reproductive maturity and sex pheromone communication in male tephritid flies in order to develop methods for acceleration of reproductive maturity among sterilized males. Our studies revealed that exposure to the juvenile hormone analogue methoprene can accelerate the rate of sexual maturity in some but not all tephritid species tested. Additionally, we have determined that incorporation of protein hydrolysate into the adult diet improves sexual performance of sterile males. Coupling a diet enriched with protein hydrolysate for adult food and application of methoprene to adult males or pupae was found to advance significantly the age at which males of a number of species of flies from the genus Anastrepha and Bactrocera cucurbitae (Coquillet) become sexually mature and improve reproductive success of the males. These results have led to the development of a novel strategy to accelerate the reproductive development and increased competitiveness of mass-reared fruit flies for use in the sterile insect technique by incorporating methoprene treatment and protein hydrolysate diets into protocols for fruit fly emergence and release facilities. The following reviews research conducted to develop the system and our suggestions for use in improving efficacy of mating by sterile males destined for release in programmes to control some species of tephritid flies using the sterile insect technique. C1 [Teal, P. E. A.] ARS, CMAVE, USDA, Gainesville, FL 32604 USA. [Pereira, R.; Haq, I.; Robinson, A. S.; Hendrichs, J.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Vienna, Austria. [Segura, D. F.] INTA Castelar, CNIA, Inst Genet EA Favret, Castelar, Buenos Aires, Argentina. [Gomez-Simuta, Y.] Programa Moscamed, Dept Desarrollo Metodos, Tapachula, Mexico. RP Teal, PEA (reprint author), ARS, CMAVE, USDA, 1700 SW 23 Dr,POB 14565, Gainesville, FL 32604 USA. EM peter.teal@ars.usda.gov OI , Ihsan/0000-0001-5879-7698; Segura, Diego/0000-0001-7714-6559 NR 49 TC 9 Z9 9 U1 3 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 91 EP 98 DI 10.1111/j.1439-0418.2010.01606.x PG 8 WC Entomology SC Entomology GA 153DW UT WOS:000319582600013 ER PT J AU Steiner, E Woods, W McInnis, DO Lindsey, J Fogliani, R Soopaya, R AF Steiner, E. Woods, W. McInnis, D. O. Lindsey, J. Fogliani, R. Soopaya, R. TI Ginger root oil increases mating competitiveness of sterile Mediterranean fruit fly (Diptera:Tephritidae) in Western Australia SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Ceratitis capitata; area-wide pest management; field cages; GRO exposure; irradiation; Sterile Insect Technique ID CERATITIS-CAPITATA DIPTERA; FIELD-CAGE TRIALS; INSECT TECHNIQUE; ALPHA-COPAENE; TEPHRITIDAE; FLIES; SUCCESS; MALES; AROMATHERAPY; COURTSHIP AB The exposure of sterile male Mediterranean fruit fly, Ceratitis capitata (Wiedemann) to the aroma of essential oil derived from ginger root Zingiber officinale Roscoe (GRO) has been shown in field cage trials to increase their mating success. This field cage study compared the mating performance of mass reared sterile Mediterranean fruit fly males prepared for ground release programmes under four different post-irradiation systems, two of which involved exposure of the flies to GRO aroma. In the first system, irradiated pupae were placed into non-vented 5-L paper tubs, individually aromatized by 20l of GRO (4ml/m3) for 96-120h until the adults were 2-3days old. In the second system, irradiated pupae were placed in vented 5-L paper tubs, exposed for 24h in a GRO aromatized room (0.5ml/m3) when the adults were 3days old and contained until required for mating tests at 5days old. The third and fourth systems were the same as the first two systems respectively, except that the flies were not exposed to GRO aroma. Significant differences in mating success were found among treatments. The exposure of 2 to 3-day old flies to GRO aroma improved mating performance slightly compared with equivalent non-exposed flies, but it was still below the minimum value accepted under international standards, while 27% of unexposed 5-day-old sterile males mated and had competitiveness (Fried value 0.38) slightly above this minimum level. GRO exposed 5-day-old flies had the highest mating percentage (67%) and a level of competitiveness (Fried value 1.81) based on induced sterility above that of wild flies (Fried value 1). The results indicate that the effectiveness of sterile male ground release programmes can be increased significantly by releasing 5-day-old sterile males that have been exposed to GRO in an entirely aromatized adult fly holding room. C1 [Steiner, E.; Woods, W.; Lindsey, J.; Fogliani, R.; Soopaya, R.] Dept Agr & Food, S Perth, WA, Australia. [McInnis, D. O.] ARS, USDA, Honolulu, HI USA. RP Steiner, E (reprint author), Dept Agr & Food, 5 Baron Hay Court, S Perth, WA, Australia. EM esteiner@agric.wa.gov.au NR 34 TC 6 Z9 6 U1 0 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 103 EP 112 DI 10.1111/j.1439-0418.2011.01630.x PG 10 WC Entomology SC Entomology GA 153DW UT WOS:000319582600015 ER PT J AU Pereira, R Yuval, B Liedo, P Teal, PEA Shelly, TE McInnis, DO Hendrichs, J AF Pereira, R. Yuval, B. Liedo, P. Teal, P. E. A. Shelly, T. E. McInnis, D. O. Hendrichs, J. TI Improving sterile male performance in support of programmes integrating the sterile insect technique against fruit flies SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Review DE abiotic environment; hormonal supplements; nutritional supplements; release methods; semiochemical supplements; Tephritidae ID CERATITIS-CAPITATA DIPTERA; GINGER ROOT OIL; ANASTREPHA-SUSPENSA DIPTERA; FIELD-CAGE TRIALS; ENHANCES MATING SUCCESS; MALE SEXUAL PERFORMANCE; ADULT HOLDING ROOMS; FLY DIPTERA; METHYL EUGENOL; DIETARY-PROTEIN AB The sterile insect technique (SIT) is being applied against fruit fly pests in many areas of the world. Currently, factories have a capacity to produce several billion sterile insects per week, to irradiate them and to make them available for shipment to their destinations. There the emerging flies are fed and maintained for various periods of time in fly emergence and release facilities, and then collected for release. While much research effort has been invested in improving mass rearing and quality control procedures at the fly factory level, the post-factory handling of sterile flies has received much less attention. A 6-year FAO/IAEA Coordinated Research Project (CRP) was initiated with the participation of 31 research institutes from 17 countries to assess and validate ways to improve sterile male performance through better management during the critical period that starts with the arrival of pupae at the fly emergence and release facility and ends with the release of the sterile flies in the field. This review summarizes the research that was conducted under the CRP focusing on fruit fly species from the genera Anastrepha, Bactrocera and Ceratitis against which the SIT is being applied. To increase the effectiveness of SIT programmes, exposure of sterile males to nutritional, hormonal and semiochemical supplements was assessed and improved handling and release methods tested. Incorporation of protein and juvenile hormone into pre-release diets significantly accelerated sterile male maturation and improved sexual performance among several species, while semiochemical treatments using ginger root oil or citrus oils in Ceratitis capitata and methyl eugenol in Bactrocera dorsalis complex species significantly increased sterile male mating competitiveness. Improved fly emergence, holding and release procedures were also assessed, together with the compilation of all this knowledge into a manual. Several of these findings have been transferred to and are being applied in operational programmes. C1 [Pereira, R.; Hendrichs, J.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, A-1400 Vienna, Austria. [Yuval, B.] Hebrew Univ Jerusalem, Dept Entomol, Jerusalem, Israel. [Liedo, P.] El Colegio Frontera Sur ECOSUR, Tapachula, Chiapas, Mexico. [Teal, P. E. A.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Shelly, T. E.] APHIS USDA, Waimanalo, HI USA. [McInnis, D. O.] ARS, USDA, Pacific Basin Agr Res Ctr, Honolulu, HI USA. RP Pereira, R (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Wagramerstr 5,POB 100, A-1400 Vienna, Austria. EM R.Cardoso-Pereira@iaea.org RI Liedo, Pablo/E-9313-2010 OI Liedo, Pablo/0000-0002-0004-1721 NR 109 TC 17 Z9 17 U1 5 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 178 EP 190 DI 10.1111/j.1439-0418.2011.01664.x PG 13 WC Entomology SC Entomology GA 153DW UT WOS:000319582600021 ER PT J AU Pereira, R Teal, PEA Conway, H Worley, J Sivinski, J AF Pereira, R. Teal, P. E. A. Conway, H. Worley, J. Sivinski, J. TI Influence of methoprene and dietary protein on maturation and sexual performance of sterile Anastrepha ludens (Diptera:Tephritidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE hydrolysed protein; juvenile hormone analogue; male competitiveness; Mexican fruit fly; sexual success ID MEDITERRANEAN FRUIT-FLIES; DIPTERA TEPHRITIDAE; SUSPENSA DIPTERA; ADULT DIET; FLY LEKS; MALES; SUCCESS; ERADICATION; SURVIVAL; BEHAVIOR AB The success of the sterile insect technique (SIT) requires the release of males that can compete with wild males. Elevated juvenile hormone (JH) levels and hydrolysed yeast-enriched adult diets improve the sexual attractiveness and competitiveness of sterile male Anastrepha ludens (Loew; Mexican fruit fly), used in large-scale SIT programmes in Mexico and Texas. We applied methoprene (a JH analogue) to A.ludens using two methods, topically on individual sterile adults and mass- immersion of pupae after irradiation. Hydrolysed yeast was either used dry or in agar blocks. Laboratory and field cage experiments were conducted to compare male maturation and sexual performance when males exposed to four different treatments competed for females: (M+P+) application of methoprene and sugar plus hydrolysed yeast as adult food; (M+P-) application of methoprene and sugar only as adult food; (M-P+) no application of methoprene and sugar plus hydrolysed yeast as adult food; and (M-P-) no application of methoprene and sugar only as adult food. Methoprene and hydrolysed yeast accelerated the male sexual maturation from 9 to 5days after the emergence. Combined application of methoprene and hydrolysed yeast had a significant effect on increased male sexual performance in both laboratory and field cage tests. Methoprene application also accelerates female maturation, although less than in males. The substantial improvements in male sexual performance and sexual maturation that can be obtained by incorporating the use of JH analogue and hydrolysed protein, and the development of techniques for their practical application on a large scale, can contribute to a more cost-effective deployment of sterile male Mexican fruit flies in SIT programmes. C1 [Pereira, R.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, A-1400 Vienna, Austria. [Teal, P. E. A.; Sivinski, J.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [Conway, H.] USDA APHIS PPQ CPHST Mission Lab, Edinburg, TX USA. [Worley, J.] USDA APHIS PPQ Mexican Fruit Fly Rearing Facil, Edinburg, TX USA. RP Pereira, R (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Wagramerstr 5,POB 100, A-1400 Vienna, Austria. EM r.cardoso-pereira@iaea.org FU International Atomic Energy Agency [12863]; USDA FX We thank to Diego Segura (INTA, Buenos Aires, Argentina) for critical review of an earlier version of this manuscript. This project was funded in part by the International Atomic Energy Agency (Research Contract 12863). Financial support to conduct the study was provided to RP by USDA. NR 42 TC 7 Z9 7 U1 2 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 191 EP 199 DI 10.1111/j.1439-0418.2011.01668.x PG 9 WC Entomology SC Entomology GA 153DW UT WOS:000319582600022 ER PT J AU Ji, QE Chen, JH McInnis, DO Guo, QL AF Ji, Q. E. Chen, J. H. McInnis, D. O. Guo, Q. L. TI The effect of methyl eugenol exposure on subsequent mating performance of sterile males of Bactrocera dorsalis SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE mating competitiveness; oriental fruit fly; parapheromone; remating; sterile males ID ORIENTAL FRUIT-FLY; INSECT TECHNIQUE; TEPHRITIDAE; DIPTERA; COMPETITIVENESS; SUCCESS; FEMALE; CONSUMPTION; ATTRACTION; INCREASES AB Methyl eugenol (ME) is ingested and used by several fruit fly species of the genus Bactrocera as a precursor of the sexual pheromone produced by adult males. This can result in an increase in male sexual activity and performance, which is important to the development of an effective sterile insect technique programme in China. The effect of ME on mating competitiveness, multiple mating, and the incidence of female remating were studied in sterile males from a genetic sexing strain of Bactrocera dorsalis (Hendel) irradiated with 100Gy of 137Cs. The results obtained in field cages showed that feeding on ME did not increase the frequency of multiple mating by sterile males, but significantly increased the mating competitiveness of sterile males against that of semi-wild males. At the same time, sterile males fed ME did not significantly affect the remating of semi-wild females 5days after the initial mating with a semi-wild male, but the ME-fed males increased the remating frequency of females 10 and 15days after the initial mating. C1 [Ji, Q. E.; Chen, J. H.; Guo, Q. L.] Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China. [McInnis, D. O.] ARS, USDA, Pacific Basin Agr Res Ctr, Honolulu, HI USA. RP Ji, QE (reprint author), Fujian Agr & Forestry Univ, Fuzhou 350002, Peoples R China. EM jiqinge@yahoo.com.cn FU Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA) [14751]; Ministry of Agriculture of P.R.C [200903047]; Fujian Provincial Department of Science Technology [2009N0017] FX This research was supported by the Food and Agriculture Organization/International Atomic Energy Agency (FAO/IAEA) through research contract No. 14751, by the Ministry of Agriculture of P.R.C through the research programme No. 200903047 and by the Fujian Provincial Department of Science & Technology through the research programme No. 2009N0017. NR 25 TC 3 Z9 4 U1 4 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 238 EP 243 DI 10.1111/j.1439-0418.2011.01686.x PG 6 WC Entomology SC Entomology GA 153DW UT WOS:000319582600027 ER PT J AU Gomez, Y Teal, PEA Pereira, R AF Gomez, Y. Teal, P. E. A. Pereira, R. TI Enhancing efficacy of Mexican fruit fly SIT programmes by large-scale incorporation of methoprene into pre-release diet SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE adult diet; Anasptrepha ludens; juvenile hormone; sexual maturation; sexual performance ID DIPTERA TEPHRITIDAE; MATING PERFORMANCE; MALES AB The juvenile hormone analogue, methoprene, has been documented to accelerate the development of reproductive competence and sexual signalling of Caribbean (Anastrepha suspensa), the Mexican (Anastrepha ludens), the South American (Anastrepha fraterculus) and West Indian (Anastrepha obliqua) tephritid fruit flies. The incorporation of methoprene into sterile fly release protocols at fly emergence and handling facilities is a key step required for large-scale application of the technology to field release strategies. The goal of our study was to develop a method to supply, in large scale, methoprene to sterile Mexican fruit flies for release in the current Sterile Insect Technique (SIT) programme in Mexico. In field cage tests, the isolation index of sterile males after treatment with methoprene was reduced, increasing the percentage of mating between laboratory sterile males and wild females. In laboratory trials, males fed a diet containing 0.05% or 0.1% methoprene mated 4-day earlier than untreated control males. In a pilot area tested in 3500ha to measure the impact of the sterile releases when methoprene was supplied, no adult wild flies were detected and no larvae were found after sampling more than 330kg of fruit both in the treated and untreated blocks. Based on the results obtained in this study, we recommend the incorporation of the methoprene on adult diet to improve the Mexican fruit fly male sexual performance when release in the field, contributing to the increase in the cost-effectiveness of the SIT programme. C1 [Gomez, Y.] Medfly Program DGSV SAGARPA, Metapa, Chiapas, Mexico. [Teal, P. E. A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Pereira, R.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Vienna, Austria. RP Pereira, R (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Vienna, Austria. EM r.cardoso-pereira@iaea.org FU International Atomic Energy Agency [OIEA/MEXICO/12861] FX This project was funded in part by the International Atomic Energy Agency (Research Contract OIEA/MEXICO/12861), acknowledgments also to Javier Coutino Ibarias for the technician support; Salvador Meza for the analysis and the field cage test and the Comite Estatal de Sanidad Vegetal de Nuevo Leon and Martin de los Santos fort their collaboration. NR 24 TC 8 Z9 8 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 SU 1 SI SI BP 252 EP 259 DI 10.1111/j.1439-0418.2011.01695.x PG 8 WC Entomology SC Entomology GA 153DW UT WOS:000319582600029 ER PT J AU LaRocco, M Krug, PW Kramer, E Ahmed, Z Pacheco, JM Duque, H Baxt, B Rodriguez, LL AF LaRocco, Michael Krug, Peter W. Kramer, Ed Ahmed, Zaheer Pacheco, Juan M. Duque, Hernando Baxt, Barry Rodriguez, Luis L. TI A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine alpha(V)beta(6) Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID NONSTRUCTURAL PROTEINS; 3A PROTEIN; HOST-RANGE; CATTLE; SENSITIVITY; INFECTION; CULTURES; RECEPTOR; SWINE; ALPHA-V-BETA-6 AB Foot-and-mouth disease (FMD) is a worldwide problem limiting the trade of animals and their products from affected countries. The rapid isolation, serotyping, and vaccine matching of FMD virus from disease outbreaks is critical for enabling the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Some primary cells have been shown to be highly susceptible to most strains of FMD virus (FMDV) but are difficult and expensive to prepare and maintain. Since the alpha(V)beta(6) integrin is a principal receptor for FMDV, we transduced a bovine kidney cell line to stably express both the alpha(V) and beta(6) bovine integrin subunits. This stable cell line (LFBK-alpha(V)beta(6)) showed beta(6) expression and enhanced susceptibility to FMDV infection for >= 100 cell passages. LFBK-alpha(V)beta(6) cells were highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophilic FMDV strain O/TAW/97. In comparison to other cell types that are currently used for virus isolation, LFBK-alpha(V)beta(6) cells were more effective at detecting FMDV in clinical samples, supporting their use as a more sensitive tool for virus isolation. C1 [LaRocco, Michael; Krug, Peter W.; Ahmed, Zaheer; Pacheco, Juan M.; Baxt, Barry; Rodriguez, Luis L.] USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Greenport, NY USA. [Kramer, Ed] McConnell Grp Inc, Rockville, MD USA. [Duque, Hernando] Anim & Plant Hlth Inspect Serv, Foreign Anim Dis Diagnost Lab, USDA, Plum Isl Anim Dis Ctr, Greenport, NY USA. RP Krug, PW (reprint author), USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, Greenport, NY USA. EM peter.krug@ars.usda.gov NR 36 TC 25 Z9 25 U1 0 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUN PY 2013 VL 51 IS 6 BP 1714 EP 1720 DI 10.1128/JCM.03370-12 PG 7 WC Microbiology SC Microbiology GA 147VV UT WOS:000319197800010 PM 23515553 ER PT J AU Bailey, RT Gates, TK Halvorson, AD AF Bailey, Ryan T. Gates, Timothy K. Halvorson, Ardell D. TI Simulating variably-saturated reactive transport of selenium and nitrogen in agricultural groundwater systems SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Selenium; Subsurface nutrient transport; Groundwater modeling; Ensemble Kalman Filter ID SOIL COLUMNS; KESTERSON RESERVOIR; IRRIGATION DRAINAGE; NITRATE TRANSPORT; STEADY-STATE; WATER; MODEL; REDUCTION; SEDIMENTS; SELENATE AB Selenium (Se) contamination in environmental systems has become a major issue in many regions world-wide during the previous decades, with both elevated and deficient Se concentrations in groundwater, surface water, soils and associated cultivated crops reported. To provide a tool that can assess baseline conditions and explore remediation strategies, this paper presents a numerical model capable of simulating the reactive transport of Se species in large-scale variably-saturated groundwater systems influenced by agricultural practices. Developed by incorporating a Se reaction module into the multi-species, variably-saturated reactive transport model UZF-RT3D, model features include near-surface Se cycling due to agricultural practices, oxidation-reduction reactions, and the inclusion of a nitrogen (N) cycle and reaction module due to the dependence of Se transformation and speciation on the presence of nitrate (NO3). Although the primary motivation is applying the model to large-scale systems, this paper presents applications to agricultural soil profile systems to corroborate the near-surface module processes that are vital in estimating mass loadings to the saturated zone in large-scale fate and transport studies. The first application jointly tests the Se and N modules for corn test plots receiving varying loadings of fertilizer, whereas the second application tests the N module for fertilized and unfertilized test plots. Results indicate that the model is successful in reproducing observed measurements of Se and NO3 concentrations, particularly in lower soil layers and hence in regards to leaching. For the first application, the Ensemble Kalman Filter (EnKF) is used to condition model parameters, demonstrating the usefulness of the EnKF in real-world reactive transport systems. (C) 2013 Elsevier B.V. All rights reserved. C1 [Bailey, Ryan T.; Gates, Timothy K.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Halvorson, Ardell D.] USDA ARS, Ft Collins, CO 80526 USA. RP Bailey, RT (reprint author), Colorado State Univ, Dept Civil & Environm Engn, 1372 Campus Delivery, Ft Collins, CO 80523 USA. EM rtbailey@engr.colostate.edu; tkg@engr.colostate.edu; ardell.halvorson@ars.usda.gov OI Bailey, Ryan/0000-0002-6539-1474 FU Colorado Agricultural Experiment Station; Colorado Department of Public Health and Environment FX This work has been made possible by grants from the Colorado Agricultural Experiment Station and the Nonpoint Source Program of the Colorado Department of Public Health and Environment. We appreciate Dr. Michael Bartolo for providing the opportunity to sample soil from the Arkansas Valley Research Center and for providing ranges of values for parameter and system forcing terms. We thank also Dr. William Hunter of USDA-ARS at the Natural Resources Research Center in Fort Collins, CO for providing laboratory space to conduct soil analysis and to Robin Montenieri for expert technical assistance. We would like to acknowledge two anonymous reviewers for their helpful comments and suggestions in improving the content of this paper. NR 89 TC 11 Z9 11 U1 5 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD JUN PY 2013 VL 149 BP 27 EP 45 DI 10.1016/j.jconhyd.2013.03.001 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA 152SC UT WOS:000319550400003 PM 23584456 ER PT J AU Hegde, NV Praul, C Gehring, A Fratamico, P DebRoy, C AF Hegde, Narasimha V. Praul, Craig Gehring, Andrew Fratamico, Pina DebRoy, Chitrita TI Rapid O serogroup identification of the six clinically relevant Shiga toxin-producing Escherichia coli by antibody microarray SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Non-O157 STEC; O serogroup identification; Antibody microarray ID ARRAY AB An antibody microarray was developed to detect the "top six" non-0157 serogroups, O26, O45, O103, O111, O121, and O145 of Shiga toxin-producing Escherichia coli (STEC), that have been declared as adulterant in meat by the Food Safety and Inspection Service of the United States Department of Agriculture. The sensitivity of the array was 10(5) CFU and the limit of detection of each serogroup in artificially inoculated ground beef was 1-10 CFU following 12 h of enrichment. Optimal concentrations of antibodies for printing and labeling and bacterial dilutions for binding to the antibodies were assessed. The array utilized a minimal amount of antibodies and other reagents and may be utilized for screening of multiple target O groups of STEC in parallel, directly from enriched samples in less than 3 h. Furthermore, the antibody array provides the flexibility to include other O serogroups of E. coli and may be adopted for high throughput screening. The method is potentially applicable to detect the pathogenic STEC O groups of E. coli in meat and other food, thus improving food safety and public health. (C) 2013 Elsevier B.V. All rights reserved. C1 [Hegde, Narasimha V.; DebRoy, Chitrita] Penn State Univ, Dept Vet & Biomed Sci, Escherichia Coli Reference Ctr, University Pk, PA 16802 USA. [Praul, Craig] Penn State Univ, Genom Core Facil, University Pk, PA 16802 USA. [Gehring, Andrew; Fratamico, Pina] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Hegde, NV (reprint author), Penn State Univ, Dept Vet & Biomed Sci, Escherichia Coli Reference Ctr, University Pk, PA 16802 USA. EM nvh1@psu.edu NR 12 TC 6 Z9 6 U1 2 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 J9 J MICROBIOL METH JI J. Microbiol. Methods PD JUN PY 2013 VL 93 IS 3 BP 273 EP 276 DI 10.1016/j.mimet.2013.03.024 PG 4 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 154BQ UT WOS:000319646900018 PM 23570904 ER PT J AU Berryman, CE Grieger, JA West, SG Chen, CYO Blumberg, JB Rothblat, GH Sankaranarayanan, S Kris-Etherton, PM AF Berryman, Claire E. Grieger, Jessica A. West, Sheila G. Chen, Chung-Yen O. Blumberg, Jeffrey B. Rothblat, George H. Sankaranarayanan, Sandhya Kris-Etherton, Penny M. TI Acute Consumption of Walnuts and Walnut Components Differentially Affect Postprandial Lipemia, Endothelial Function, Oxidative Stress, and Cholesterol Efflux in Humans with Mild Hypercholesterolemia SO JOURNAL OF NUTRITION LA English DT Article ID COA DESATURASE 1; CARDIOVASCULAR RISK; FOAM CELLS; TREE NUTS; CAPACITY; PLASMA; OIL; PREVENTION; CROSSOVER; RECEPTOR AB Walnut consumption improves cardiovascular disease risk; however, to our knowledge, the contribution of individual walnut components has not been assessed. This study evaluated the acute consumption of whole walnuts (85 g), separated nut skins (5.6 g), de-fatted nutmeat (34 g), and nut oil (51 g) on postprandial lipemia, endothelial function, and oxidative stress. Cholesterol efflux (ex vivo) was assessed in the whole walnut treatment only. A randomized, 4-period, crossover trial was conducted in healthy overweight and obese adults (n = 15) with moderate hypercholesterolemia. There was a treatment x time point interaction for triglycerides (P < 0.01) and increased postprandial concentrations were observed for the oil and whole walnut treatments (P < 0.01). Walnut skins decreased the reactive hyperemia index (RHI) compared with baseline (P = 0.02) such that a difference persisted between the skin and oil treatments (P = 0.01). The Framingham RH I was maintained with the oil treatment compared with the skins and whole nut (P < 0.05). There was a treatment effect for the ferric reducing antioxidant potential (FRAP) (P < 0.01), and mean FRAP was greater with the oil and skin treatments compared with the nutmeat (P < 0.01). Cholesterol efflux increased by 3.3% following whole walnut consumption in J774 cells cultured with postprandial serum compared with fasting baseline (P = 0.02). Walnut oil favorably affected endothelial function and whole walnuts increased cholesterol efflux. These 2 novel mechanisms may explain in part the cardiovascular benefits of walnuts. C1 [Berryman, Claire E.; Grieger, Jessica A.; West, Sheila G.; Kris-Etherton, Penny M.] Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. [West, Sheila G.] Penn State Univ, Dept Biobehav Hlth, University Pk, PA 16802 USA. [Chen, Chung-Yen O.; Blumberg, Jeffrey B.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Rothblat, George H.; Sankaranarayanan, Sandhya] Childrens Hosp Philadelphia, Dept Pediat, Philadelphia, PA 19104 USA. RP Kris-Etherton, PM (reprint author), Penn State Univ, Dept Nutr Sci, University Pk, PA 16802 USA. EM pmk3@psu.edu RI Berryman, Claire/B-5553-2017 FU California Walnut Commission; NIH [M01 RR 10732] FX Supported by the California Walnut Commission, which had no role in the design and conduct of the study; in the collection, analysis, and interpretation of the data; or in the preparation, review, or approval of the manuscript. The General Clinical Research Center of the Pennsylvania State University was supported by NIH grant M01 RR 10732. NR 38 TC 26 Z9 26 U1 2 U2 19 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JUN PY 2013 VL 143 IS 6 BP 788 EP 794 DI 10.3945/jn.112.170993 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 149GE UT WOS:000319306200005 PM 23616506 ER PT J AU Nair, KM Brahmam, GNV Radhika, MS Dripta, RC Ravinder, P Balakrishna, N Chen, ZS Hawthorne, KM Abrams, SA AF Nair, Krishnapillai Madhavan Brahmam, Ginnela N. V. Radhika, Madhari S. Dripta, Roy Choudhury Ravinder, Punjal Balakrishna, Nagalla Chen, Zhensheng Hawthorne, Keli M. Abrams, Steven A. TI Inclusion of Guava Enhances Non-Heme Iron Bioavailability but Not Fractional Zinc Absorption from a Rice-Based Meal in Adolescents SO JOURNAL OF NUTRITION LA English DT Article ID ASCORBIC-ACID; SERUM HEPCIDIN; DIETARY IRON; WOMEN; PLASMA; BLOOD; CHILDREN; BALANCE; COPPER; FOODS AB Assessing the bioavailability of non-heme iron and zinc is essential for recommending diets that meet the increased growth-related demand for these nutrients. We studied the bioavailability of iron and zinc from a rice-based meal in 16 adolescent boys and girls, 13-15 y of age, from 2 government-run residential schools. Participants were given a standardized rice meal (regular) and the same meal with 100 g of guava fruit (modified) with Fe-57 on 2 consecutive days. A single oral dose of Fe-58 in orange juice was given at a separate time as a reference dose. Zinc absorption was assessed by using Zn-70, administered intravenously, and Zn-67 given orally with meals. The mean hemoglobin concentration was similar in girls (129 +/- 7.8 g/L) and boys (126 +/- 7.1 g/L). There were no sex differences in the indicators of iron and zinc status except for a higher hepcidin concentration in boys (P < 0.05). The regular and modified meals were similar in total iron (10-13 mg/meal) and zinc (2.7 mg/meal) content. The molar ratio of iron to phytic acid was >1:1, but the modified diet had 20 times greater ascorbic acid content. The absorption of Fe-57 from the modified meal, compared with regular meal, was significantly (P < 0.05) greater in both girls (23.9 +/- 11.2 vs. 9.7 +/- 6.5%) and boys (19.2 +/- 8.4 vs. 8.6 +/- 4.1%). Fractional zinc absorption was similar between the regular and modified meals in both sexes. Hepcidin was found to be a significant predictor of iron absorption (standardized beta = -0.63, P = 0.001, R-2 = 0.40) from the reference dose. There was no significant effect of sex on iron and zinc bioavailability from meals. We conclude that simultaneous ingestion of guava fruit with a habitual rice-based meal enhances iron bioavailability in adolescents. C1 [Nair, Krishnapillai Madhavan; Dripta, Roy Choudhury; Ravinder, Punjal] Indian Council Med Res, Natl Inst Nutr, Div Micronutrient Res, Hyderabad 500007, Andhra Pradesh, India. [Brahmam, Ginnela N. V.; Radhika, Madhari S.] Indian Council Med Res, Natl Inst Nutr, Div Community Studies, Hyderabad 500007, Andhra Pradesh, India. [Balakrishna, Nagalla] Indian Council Med Res, Natl Inst Nutr, Dept Biostat, Hyderabad 500007, Andhra Pradesh, India. [Chen, Zhensheng; Hawthorne, Keli M.; Abrams, Steven A.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Chen, Zhensheng; Hawthorne, Keli M.; Abrams, Steven A.] Texas Childrens Hosp, Houston, TX 77030 USA. RP Nair, KM (reprint author), Indian Council Med Res, Natl Inst Nutr, Div Micronutrient Res, Jamai Osmania Po, Hyderabad 500007, Andhra Pradesh, India. EM nairthayil@gmail.com OI Abrams, Steven/0000-0003-4972-9233 FU Indian Council of Medical Research [63/4/Indo-US/2003-RHN]; National Institutes of Health [HD061032] FX Supported by grants from Indian Council of Medical Research (project no.63/4/Indo-US/2003-RHN) and the National Institutes of Health (HD061032). NR 54 TC 4 Z9 5 U1 0 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD JUN PY 2013 VL 143 IS 6 BP 852 EP 858 DI 10.3945/jn.112.171702 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 149GE UT WOS:000319306200013 PM 23596161 ER PT J AU Gahche, JJ Bailey, RL Mirel, LB Dwyer, JT AF Gahche, Jaime J. Bailey, Regan L. Mirel, Lisa B. Dwyer, Johanna T. TI The Prevalence of Using Iodine-Containing Supplements Is Low among Reproductive-Age Women, NHANES 1999-2006 SO JOURNAL OF NUTRITION LA English DT Article ID AMERICAN-THYROID-ASSOCIATION; UNITED-STATES; NATIONAL-HEALTH; NUTRITION; DEFICIENCY; PREGNANCY; RECOMMENDATIONS; TRENDS AB During pregnancy, the iodine requirement rises to meet demands for neurological development and fetal growth. If these requirements are not met, irreversible pathological cognitive and behavioral changes to the fetus may ensue. This study estimated the prevalence of iodine-containing dietary supplement (DS) use and intakes of iodine from DSs among pregnant women and nonpregnant women of reproductive age (15-39 y) who were interviewed and examined in NHANES 1999-2006 (n = 6404). Although 77.5% of pregnant women reported taking one or more DSs in the past 30 d, only 22.3% consumed an iodine-containing supplement. Most pregnant women reported using one DS and reported taking this product daily: The vast majority of iodine-containing DSs reported by pregnant women claimed an iodine content of 150 mu g iodine/serving on the label. Pregnant women using at least one DS containing iodine had a mean daily iodine intake of 122 mu g/d from supplements; the median value was 144 mu g/d. Median urinary iodine concentrations (UICs) were similar for pregnant and nonpregnant women in the population aged 15-39 y. The median UIC was 148 mu g/L for pregnant women and 133 mu g/L for nonpregnant women. The WHO has established a cutoff for insufficient iodine intake at <150 mu g/L for pregnant women and <100 mg/L for those who are not pregnant. This suggests that as a population, we may not be meeting adequate intakes of iodine for pregnant women. More research is needed on the iodine intakes of pregnant women and women of reproductive age on their total iodine intake from all sources, not just DSs. C1 [Gahche, Jaime J.; Mirel, Lisa B.] Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Div Natl Hlth & Nutr Examinat Surveys, Hyattsville, MD 20782 USA. [Bailey, Regan L.; Dwyer, Johanna T.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Gahche, JJ (reprint author), Ctr Dis Control & Prevent, Natl Ctr Hlth Stat, Div Natl Hlth & Nutr Examinat Surveys, Hyattsville, MD 20782 USA. EM dvt4@cdc.gov OI Dwyer, Johanna/0000-0002-0783-1769 NR 26 TC 11 Z9 13 U1 1 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD JUN PY 2013 VL 143 IS 6 BP 872 EP 877 DI 10.3945/jn.112.169326 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 149GE UT WOS:000319306200016 PM 23616501 ER PT J AU Benight, NM Stoll, B Olutoye, OO Holst, JJ Burrin, DG AF Benight, Nancy M. Stoll, Barbara Olutoye, Oluyinka O. Holst, Jens J. Burrin, Douglas G. TI GLP-2 Delays but Does Not Prevent the Onset of Necrotizing Enterocolitis in Preterm Pigs SO JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION LA English DT Article DE inflammation; neonate; serum amyloid A ID GLUCAGON-LIKE PEPTIDE-2; SHORT-BOWEL SYNDROME; TOTAL PARENTERAL-NUTRITION; INTESTINAL GROWTH; NEONATAL PIGS; BLOOD-FLOW; DISEASE; MODEL; INFLAMMATION; PIGLETS AB Objectives: Necrotizing enterocolitis (NEC) is complex disease thought to occur as a result of an immaturity of the gastrointestinal tract of preterm infants. Intestinal dysfunction induced by total parental nutrition (TPN) may increase the risk for NEC upon introduction of enteral feeding. We hypothesized that the intestinal trophic and anti-inflammatory actions previously ascribed to the gut hormone, glucagon-like peptide-2 (GLP-2), would reduce the incidence of NEC when given in combination with TPN in preterm piglets. Methods: Preterm, newborn piglets were nourished by TPN and infused continuously with either human GLP-2 (100 mu g . kg(-1) . day(-1)) or control saline for 2 days (n = 12/group). On day 3, TPN was discontinued and pigs were given orogastric formula feeding every 3 hours, and continued GLP-2 or control treatment until the onset of clinical signs of NEC for an additional 96 hours and tissue was collected for molecular and histological endpoints. Results: GLP-2 treatment delayed the onset of NEC but was unable to prevent a high NEC incidence (similar to 70%) and severity that occurred in both groups. GLP-2-treated pigs had less histological injury and increased proximal intestinal weight and mucosal villus height, but not crypt depth or Ki-67-positive cells. Inflammatory markers of intestinal myeloperoxidase were unchanged and serum amyloid A levels were higher in GLP-2-treated pigs. Conclusions: GLP-2 did not prevent NEC and a proinflammatory response despite some reduction in mucosal injury and increased trophic effect. C1 [Benight, Nancy M.; Stoll, Barbara; Burrin, Douglas G.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Sect Pediat Gastroenterol Hepatol & Nutr,Dept Ped, Houston, TX 77030 USA. [Olutoye, Oluyinka O.] Texas Childrens Hosp, Baylor Coll Med, Michael E DeBakey Dept Surg, Div Pediat Surg, Houston, TX 77030 USA. [Holst, Jens J.] Univ Copenhagen, Dept Biomed Sci, Copenhagen, Denmark. RP Burrin, DG (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM dburrin@bcm.edu OI Benight, Nancy/0000-0001-7321-3187; Holst, Jens Juul/0000-0001-6853-3805 FU US Department of Agriculture, Agricultural Research Service [58-6250-6-001]; Texas Medical Center Digestive Diseases Center (NIH Grant) [P30 DK-56338] FX This work was supported by federal funds from the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6-001, and the Texas Medical Center Digestive Diseases Center (NIH Grant P30 DK-56338). The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 44 TC 7 Z9 8 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0277-2116 J9 J PEDIATR GASTR NUTR JI J. Pediatr. Gastroenterol. Nutr. PD JUN PY 2013 VL 56 IS 6 BP 623 EP 630 DI 10.1097/MPG.0b013e318286891e PG 8 WC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics SC Gastroenterology & Hepatology; Nutrition & Dietetics; Pediatrics GA 152VP UT WOS:000319559800020 PM 23343934 ER PT J AU Lewis, CE Reising, MM Fry, AM Conrad, SK Siev, D Gatewood, DM Hermann, JR AF Lewis, C. E. Reising, M. M. Fry, A. M. Conrad, S. K. Siev, D. Gatewood, D. M. Hermann, J. R. TI Evaluation of a non-invasive, inhalational challenge method for rabies vaccine potency assay SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Rabies virus; Rabies vaccine; NIH potency test; Vaccination challenge; Intranasal challenge ID MICE; VIRUS; ROUTE AB Veterinary rabies vaccines are essential for safeguarding the public from exposure to rabies virus, as vaccination of domestic animals provides a barrier between humans and wildlife reservoirs. Ensuring rabies vaccines are potent and effective is paramount in preventing human exposure to rabies virus. The National Institutes of Health (NIH) test, a mouse vaccination-challenge assay, is the most widely used and internationally recognized assay for potency testing of inactivated rabies vaccines, and it is currently considered the method of choice. In the NIH test, vaccinated mice are challenged by the intracranial (IC) route. The response to the IC challenge can be variable, which often results in invalid tests. In addition, the IC challenge-exposure raises animal welfare concerns. The objective of this study was to evaluate the intranasal route of challenge as a modification to the NIH test to reduce animal pain and suffering until harmonized requirements for in vitro testing of rabies vaccines are developed. Results confirm the intranasal route is an effective route of rabies challenge in mice. However, a valid challenge requires the use of a more concentrated inoculum, in comparison to the intracranial method. Published by Elsevier B.V. C1 [Lewis, C. E.; Reising, M. M.; Fry, A. M.; Conrad, S. K.; Siev, D.; Gatewood, D. M.; Hermann, J. R.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Vet Biol, Ames, IA 50010 USA. RP Hermann, JR (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Ctr Vet Biol, 1920 Dayton Ave,POB 844, Ames, IA 50010 USA. EM joseph.r.hermann@aphis.usda.gov NR 19 TC 1 Z9 1 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 J9 J VIROL METHODS JI J. Virol. Methods PD JUN PY 2013 VL 190 IS 1-2 BP 49 EP 52 DI 10.1016/j.jviromet.2013.03.020 PG 4 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA 153XU UT WOS:000319636900009 PM 23557669 ER PT J AU Gladue, DP O'Donnell, V Baker-Branstetter, R Holinka, LG Pacheco, JM Sainz, IF Lu, Z Ambroggio, X Rodriguez, L Borca, MV AF Gladue, D. P. O'Donnell, V. Baker-Branstetter, R. Holinka, L. G. Pacheco, J. M. Sainz, I. Fernandez Lu, Z. Ambroggio, X. Rodriguez, L. Borca, M. V. TI Foot-and-Mouth Disease Virus Modulates Cellular Vimentin for Virus Survival SO JOURNAL OF VIROLOGY LA English DT Article ID VIRAL REPLICATION COMPLEX; INTERMEDIATE-FILAMENT CYTOSKELETON; PROTEIN-PROTEIN INTERACTIONS; JAPANESE ENCEPHALITIS-VIRUS; 2-HYBRID SYSTEM; VACCINIA VIRUS; CELLS; MICROTUBULES; INFECTION; SITE AB Foot-and-mouth disease virus (FMDV), the causative agent of foot-and-mouth disease, is an Aphthovirus within the Picornaviridae family. During infection with FMDV, several host cell membrane rearrangements occur to form sites of viral replication. FMDV protein 2C is part of the replication complex and thought to have multiple roles during virus replication. To better understand the role of 2C in the process of virus replication, we have been using a yeast two-hybrid approach to identify host proteins that interact with 2C. We recently reported that cellular Beclin1 is a natural ligand of 2C and that it is involved in the autophagy pathway, which was shown to be important for FMDV replication. Here, we report that cellular vimentin is also a specific host binding partner for 2C. The 2C-vimentin interaction was further confirmed by coimmunoprecipitation and immunofluorescence staining to occur in FMDV-infected cells. It was shown that upon infection a vimentin structure forms around 2C and that this structure is later resolved or disappears. Interestingly, overexpression of vimentin had no effect on virus replication; however, overexpression of a truncated dominant-negative form of vimentin resulted in a significant decrease in viral yield. Acrylamide, which causes disruption of vimentin filaments, also inhibited viral yield. Alanine scanning mutagenesis was used to map the specific amino acid residues in 2C critical for vimentin binding. Using reverse genetics, we identified 2C residues that are necessary for virus growth, suggesting that the interaction between FMDV 2C and cellular vimentin is essential for virus replication. C1 [Gladue, D. P.; O'Donnell, V.; Baker-Branstetter, R.; Holinka, L. G.; Pacheco, J. M.; Sainz, I. Fernandez; Rodriguez, L.; Borca, M. V.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Lu, Z.] DHS, Plum Isl Anim Dis Ctr, Greenport, NY USA. [Ambroggio, X.] NIAID, Bioinformat & Computat Biosci Branch, NIH, Bethesda, MD 20892 USA. RP Borca, MV (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. EM pmanuel.borca@ars.usda.gov RI Fernandez Sainz, Ignacio/I-3046-2016; OI Gladue, Douglas/0000-0002-7894-0233; Pacheco, Juan/0000-0001-5477-0201; Borca, Manuel/0000-0002-0888-1178 NR 59 TC 8 Z9 8 U1 0 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD JUN PY 2013 VL 87 IS 12 BP 6794 EP 6803 DI 10.1128/JVI.00448-13 PG 10 WC Virology SC Virology GA 152CW UT WOS:000319508600025 PM 23576498 ER PT J AU Volpi, E Campbell, WW Dwyer, JT Johnson, MA Jensen, GL Morley, JE Wolfe, RR AF Volpi, Elena Campbell, Wayne W. Dwyer, Johanna T. Johnson, Mary Ann Jensen, Gordon L. Morley, John E. Wolfe, Robert R. TI Is the Optimal Level of Protein Intake for Older Adults Greater Than the Recommended Dietary Allowance? SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article ID ESSENTIAL AMINO-ACIDS; SKELETAL-MUSCLE; WEIGHT-LOSS; SARCOPENIC OBESITY; NITROGEN-BALANCE; BODY-COMPOSITION; NATIONAL-HEALTH; ELDERLY-PEOPLE; YOUNG MEN; REQUIREMENTS AB Protein is a macronutrient essential for growth, muscle function, immunity and overall tissue homeostasis. Suboptimal protein intake can significantly impact physical function and overall health in older adults. This article reviews the literature on the recommendations for protein intake in older adults in light of the new evidence linking protein intake with sarcopenia and physical function. Challenges and opportunities for optimal protein nutrition in older persons are discussed. Recent metabolic and epidemiological studies suggest that the current recommendations of protein intake may not be adequate for maintenance of physical function and optimal health in older adults. Methodological limitations and novel concepts in protein nutrition are also discussed. We conclude that new research and novel research methodologies are necessary to establish the protein needs and optimal patterns of protein intake for older persons. C1 [Volpi, Elena] Univ Texas Med Branch, Dept Internal Med, Galveston, TX 77555 USA. [Volpi, Elena] Univ Texas Med Branch, Dept Nutr & Metab, Galveston, TX 77555 USA. [Volpi, Elena] Univ Texas Med Branch, Sealy Ctr Aging, Galveston, TX 77555 USA. [Campbell, Wayne W.] Purdue Univ, W Lafayette, IN 47907 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA USA. [Johnson, Mary Ann] Univ Georgia, Athens, GA 30602 USA. [Jensen, Gordon L.] Penn State Univ, University Pk, PA 16802 USA. [Morley, John E.] St Louis Univ, St Louis, MO 63103 USA. [Wolfe, Robert R.] Univ Arkansas Med Sci, Little Rock, AR 72205 USA. Univ Texas Med Branch, Galveston, TX 77555 USA. RP Volpi, E (reprint author), Univ Texas Med Branch, 301 Univ Blvd, Galveston, TX 77555 USA. EM evolpi@utmb.edu OI Volpi, Elena/0000-0001-8776-0384; Dwyer, Johanna/0000-0002-0783-1769 FU American Federation for Aging Research (AFAR); National Institute on Aging [P30 AG024832] FX This article summarizes the conclusions of an expert panel discussion organized and funded by the American Federation for Aging Research (AFAR). This article was also supported in part by the National Institute on Aging grant number P30 AG024832. NR 49 TC 82 Z9 82 U1 6 U2 38 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JUN PY 2013 VL 68 IS 6 BP 677 EP 681 DI 10.1093/gerona/gls229 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 151NA UT WOS:000319466000006 PM 23183903 ER PT J AU Chale, A Cloutier, GJ Hau, C Phillips, EM Dallal, GE Fielding, RA AF Chale, Angela Cloutier, Gregory J. Hau, Cynthia Phillips, Edward M. Dallal, Gerard E. Fielding, Roger A. TI Efficacy of Whey Protein Supplementation on Resistance ExerciseInduced Changes in Lean Mass, Muscle Strength, and Physical Function in Mobility-Limited Older Adults SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Nutrition; Physical function; Sarcopenia ID RANDOMIZED CONTROLLED-TRIAL; SKELETAL-MUSCLE; ELDERLY HUMANS; YOUNG MEN; HYPERTROPHY; PERFORMANCE; WOMEN; POWER; SIZE; LIMITATIONS AB Whey protein supplementation may augment resistance exercise-induced increases in muscle strength and mass. Further studies are required to determine whether this effect extends to mobility-limited older adults. The objectives of the study were to compare the effects of whey protein concentrate (WPC) supplementation to an isocaloric control on changes in whole-body lean mass, mid-thigh muscle cross-sectional area, muscle strength, and stair-climbing performance in older mobility-limited adults in response to 6 months of resistance training (RT). Eighty mobility-limited adults aged 7085 years were randomized to receive WPC (40g/day) or an isocaloric control for 6 months. All participants also completed a progressive high-intensity RT intervention. Sample sizes were calculated based on the primary outcome of change in whole-body lean mass to give 80% power for a 0.05-level, two-sided test. Lean mass increased 1.3% and 0.6% in the WPC and control groups, respectively. Muscle cross-sectional area was increased 4.6% and 2.9% in the WPC and control groups, respectively, and muscle strength increased 16%50% in WPC and control groups. Stair-climbing performance also improved in both groups. However, there were no statistically significant differences in the change in any of these variables between groups. These data suggest that WPC supplementation at this dose does not offer additional benefit to the effects of RT in mobility-limited older adults. C1 [Chale, Angela; Cloutier, Gregory J.; Hau, Cynthia; Phillips, Edward M.; Dallal, Gerard E.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Phillips, Edward M.] Harvard Univ, Sch Med, Spaulding Rehabil Hosp, Dept Phys Med & Rehabil, Boston, MA USA. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington Ave, Boston, MA 02111 USA. EM roger.fielding@tufts.edu FU Dairy Research Institute; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; Boston Nutrition/Obesity Research Center [DK046200]; U.S. Department of Agriculture [58-1950-0-014]; [DK007651] FX This work was supported by Dairy Research Institute (R.A.F.), Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679 to R.A.F.), the Boston Nutrition/Obesity Research Center (DK046200 to R.A.F.), a postdoctoral training grant (DK007651 to A.C.), and the U.S. Department of Agriculture under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 32 TC 43 Z9 44 U1 7 U2 55 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JUN PY 2013 VL 68 IS 6 BP 682 EP 690 DI 10.1093/gerona/gls221 PG 9 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 151NA UT WOS:000319466000007 PM 23114462 ER PT J AU Gribok, A Hoyt, R Buller, M Rumpler, W AF Gribok, Andrei Hoyt, Reed Buller, Mark Rumpler, William TI On the accuracy of instantaneous gas exchange rates, energy expenditure and respiratory quotient calculations obtained from indirect whole room calorimetry SO PHYSIOLOGICAL MEASUREMENT LA English DT Article; Proceedings Paper CT 13th International Conference on Biomedical Applications of Electrical Impedance Tomography (EIT) CY MAY 23-25, 2012 CL Tianjin, PEOPLES R CHINA DE indirect calorimetry; uncertainty; regularization ID BODY INDIRECT CALORIMETER; TRANSIENT-RESPONSE; DECONVOLUTION; CHAMBER AB This paper analyzes the accuracy of metabolic rate calculations performed in the whole room indirect calorimeter using the molar balance equations. The equations are treated from the point of view of cause-effect relationship where the gaseous exchange rates representing the unknown causes need to be inferred from a known, noisy effect-gaseous concentrations. Two methods of such inference are analyzed. The first method is based on the previously published regularized deconvolution of the molar balance equation and the second one, proposed in this paper, relies on regularized differentiation of gaseous concentrations. It is found that both methods produce similar results for the absolute values of metabolic variables and their accuracy. The uncertainty for O-2 consumption rate is found to be 7% and for CO2 production-3.2%. The uncertainties in gaseous exchange rates do not depend on the absolute values of O-2 consumption and CO2 production. In contrast, the absolute uncertainty in respiratory quotient is a function of the gaseous exchange rates and varies from 9.4% during the night to 2.3% during moderate exercise. The uncertainty in energy expenditure was found to be 5.9% and independent of the level of gaseous exchange. For both methods, closed form analytical formulas for confidence intervals are provided allowing quantification of uncertainty for four major metabolic variables in real world studies. C1 [Gribok, Andrei; Rumpler, William] ARS, Food Components & Hlth Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Hoyt, Reed; Buller, Mark] USA, Biophys & Biomed Modeling Div, Environm Med Res Inst, Natick, MA 01760 USA. RP Gribok, A (reprint author), ARS, Food Components & Hlth Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM agribok@utk.edu NR 22 TC 1 Z9 1 U1 1 U2 12 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0967-3334 EI 1361-6579 J9 PHYSIOL MEAS JI Physiol. Meas. PD JUN PY 2013 VL 34 IS 6 BP 737 EP 755 DI 10.1088/0967-3334/34/6/737 PG 19 WC Biophysics; Engineering, Biomedical; Physiology SC Biophysics; Engineering; Physiology GA 152WS UT WOS:000319563000013 PM 23719329 ER PT J AU Chapman, KD Dyer, JM Mullen, RT AF Chapman, Kent D. Dyer, John M. Mullen, Robert T. TI Commentary: Why don't plant leaves get fat? SO PLANT SCIENCE LA English DT Review DE Bioenergy; Lipid metabolism; Storage lipids; Leaves; Carbohydrate metabolism ID BRASSICA-NAPUS; BETA-OXIDATION; ACID SYNTHESIS; DIACYLGLYCEROL ACYLTRANSFERASE; FUNCTIONAL-DIFFERENTIATION; ARABIDOPSIS-THALIANA; STARCH TURNOVER; BUNDLE-SHEATH; METABOLISM; ACCUMULATION AB Recent pressures to obtain energy from plant biomass have encouraged new metabolic engineering strategies that focus on accumulating lipids in vegetative tissues at the expense of lignin, cellulose and/or carbohydrates. There are at least three important factors that support this rationale. (i) Lipids are more reduced than carbohydrates and so they have more energy per unit of mass. (ii) Lipids are hydrophobic and thus take up less volume than hydrated carbohydrates on a mass basis for storage in tissues. (iii) Lipids are more easily extracted and converted into useable biofuels than cellulosic-derived fuels, which require extensive fractionation, degradation of lignocellulose and fermentation of plant tissues. However, while vegetative organs such as leaves are the majority of harvestable biomass and would be ideal for accumulation of lipids, they have evolved as "source" tissues that are highly specialized for carbohydrate synthesis and export and do not have a propensity to accumulate lipid. Metabolism in leaves is directed mostly toward the synthesis and export of sucrose, and engineering strategies have been devised to divert the flow of photosynthetic carbon from sucrose, starch, lignocellulose, etc. toward the accumulation of triacylglycerols in non-seed, vegetative tissues for bioenergy applications. (C) 2013 Elsevier Ireland Ltd. All rights reserved. C1 [Chapman, Kent D.] Univ N Texas, Ctr Plant Lipid Res, Dept Biol Sci, Denton, TX 76203 USA. [Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Mullen, Robert T.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. RP Chapman, KD (reprint author), Univ N Texas, Dept Biol Sci, 1155 Union Circle 305220, Denton, TX 76206 USA. EM chapman@unt.edu FU U.S. Department of Energy, Office of Science, Division of Biological and Environmental Research FX We thank Ian Tetlow (University of Guelph) for his helpful comments on the content of the manuscript. We thank Charlene Case for assistance with manuscript preparation. The authors' research on the regulation of storage lipid accumulation in leaves is supported by the U.S. Department of Energy, Office of Science, Division of Biological and Environmental Research. NR 51 TC 37 Z9 37 U1 1 U2 80 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD JUN PY 2013 VL 207 BP 128 EP 134 DI 10.1016/j.plantsci.2013.03.003 PG 7 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 151VJ UT WOS:000319488200016 PM 23602107 ER PT J AU Zhang, W Sangtong, V Peterson, J Scott, MP Messing, J AF Zhang, Wei Sangtong, Vavaporn Peterson, Joan Scott, M. Paul Messing, Joachim TI Divergent properties of prolamins in wheat and maize SO PLANTA LA English DT Article DE Cysteines; Dx5; Endosperm; Suborganelles; Zeins ID ZEIN PROTEIN; ENDOSPERM; GENE; SEED; EXPRESSION; GLUTENIN; STORAGE; GRAIN AB Cereal grains are an important nutritional source of amino acids for humans and livestock worldwide. Wheat, barley, and oats belong to a different subfamily of the grasses than rice and in addition to maize, millets, sugarcane, and sorghum. All their seeds, however, are largely devoid of free amino acids because they are stored during dormancy in specialized storage proteins. Prolamins, the major class of storage proteins in cereals with preponderance of proline and glutamine, are synthesized at the endoplasmic reticulum during seed development and deposited into subcellular structures of the immature endosperm, the protein bodies. Prolamins have diverged during the evolution of the grass family in their structure and their properties. Here, we used the expression of wheat glutenin-Dx5 in maize to examine its interaction with maize prolamins during endosperm development. Ectopic expression of Dx5 alters protein body morphology in a way that resembles non-vitreous kernel phenotypes, although Dx5 alone does not cause an opaque phenotype. However, if we lower the amount of gamma-zeins in Dx5 maize through RNAi, a non-vitreous phenotype emerges and the deformation on the surface of protein bodies is enhanced, indicating that Dx5 requires gamma-zeins for its proper subcellular organization in maize. C1 [Zhang, Wei; Messing, Joachim] Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 USA. [Sangtong, Vavaporn; Peterson, Joan; Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Messing, J (reprint author), Rutgers State Univ, Waksman Inst Microbiol, 190 Frelinghuysen Rd, Piscataway, NJ 08854 USA. EM messing@waksman.rutgers.edu RI Zhang, Wei/H-1011-2015 FU Selman A. Waksman Chair in Molecular Genetics FX We want to thank Dr. Yongrui Wu of the Waksman Institute of Rutgers University, Piscataway, NJ, USA, for beta RNAi/+, gamma RNAi/+ transgenic seeds, his assistance and useful discussions. The research described in this manuscript was supported by the Selman A. Waksman Chair in Molecular Genetics to JM. NR 19 TC 6 Z9 6 U1 3 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 J9 PLANTA JI Planta PD JUN PY 2013 VL 237 IS 6 BP 1465 EP 1473 DI 10.1007/s00425-013-1857-5 PG 9 WC Plant Sciences SC Plant Sciences GA 151QB UT WOS:000319474200005 PM 23435659 ER PT J AU Vick, BD Moss, TA AF Vick, Brian D. Moss, Tim A. TI Adding concentrated solar power plants to wind farms to achieve a good utility electrical load match SO SOLAR ENERGY LA English DT Article DE Wind farm; Utility electrical loading; Concentrating solar; Hybrid; Solar thermal storage AB Texas has the greatest installed wind turbine capacity of any state in the United States, but as the percentage of wind generation approaches 10% of the utilities total electrical generation (in 2012, the total wind generated electricity in Texas was 7.4%), it becomes increasingly difficult for the utility to balance the electrical load due to the mismatch between the wind farm (WF) generated electricity and the utility electrical loading. In this paper WF output was shown to be diurnally and seasonally mismatched with the utility electrical loading in the Texas Panhandle (e.g. Texas Panhandle has the highest wind energy resource in Texas). In addition, the wind farm output in the Texas Panhandle does not normally contribute significantly at the peak hourly electrical load, and the peak hourly electrical load is a major deciding factor for a utility to add new power plants. A financial analysis was also performed on all the renewable energy systems analyzed. Various ratios of wind farm output to concentrating solar power (CSP) parabolic trough plant output (with 6 h of thermal storage) were calculated for the Texas Panhandle and compared to the utility electrical loading on an annual and peak monthly basis (each renewable energy system was analyzed at a 100 MW rating). The 67 MW wind farm and the 33 MW CSP plant with 6 h of thermal storage was approximately the best match to the utility electrical loading. The utility electrical load was also compared to: a 100 MW WF, a 100 MW CSP plant (with and without 6 h thermal storage), and finally the 67 MW WF with 33 MW CSP plant (with 6 h of thermal storage) on an annual, monthly, and peak hourly load basis. Typically for each month, the wind farm did not match the utility electrical loading except in the evening while the CSP plant (without storage) matched the utility electrical loading with the exception of in the evening. For the peak utility electrical loading months (July and August) and the days with the peak electrical loadings during those months, the 100 MW CSP plant with 6 h of thermal storage performed best in terms of supporting the utility electrical load (e.g. no wind farm). For the Texas Panhandle the estimated levelized cost of energy (LCOE) of a hybrid WF/CSP plant was in the range of $108/MW h to $129/MW h while the WF only system was estimated to be $64/MW h, but the benefits of adding CSP may justify the additional cost. Although the Texas Panhandle was the only location analyzed for combining CSP plants with WFs, the analysis described in this paper can be used for other regions, states, or countries. Published by Elsevier Ltd. C1 [Vick, Brian D.] ARS, USDA, Bushland, TX 79012 USA. [Moss, Tim A.] Sandia Natl Labs, Albuquerque, NM 87123 USA. RP Vick, BD (reprint author), ARS, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM brian.vick@ars.usda.gov NR 24 TC 9 Z9 9 U1 1 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-092X J9 SOL ENERGY JI Sol. Energy PD JUN PY 2013 VL 92 BP 298 EP 312 DI 10.1016/j.solener.2013.03.007 PG 15 WC Energy & Fuels SC Energy & Fuels GA 154AS UT WOS:000319644500029 ER PT J AU Chang, S Hartman, GL Singh, RJ Lambert, KN Hobbs, HA Domier, LL AF Chang, Sungyul Hartman, Glen L. Singh, Ram J. Lambert, Kris N. Hobbs, Houston A. Domier, Leslie L. TI Identification of high-quality single-nucleotide polymorphisms in Glycine latifolia using a heterologous reference genome sequence SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID SCLEROTINIA STEM ROT; GENETIC-LINKAGE MAP; ALFALFA-MOSAIC-VIRUS; PHAKOPSORA-PACHYRHIZI; PARTIAL RESISTANCE; GENUS GLYCINE; SOYBEAN RUST; WHITE MOLD; GEOGRAPHIC-DISTRIBUTION; INTERSPECIFIC HYBRIDS AB Like many widely cultivated crops, soybean [Glycine max (L.) Merr.] has a relatively narrow genetic base, while its perennial distant relatives in the subgenus Glycine Willd. are more genetically diverse and display desirable traits not present in cultivated soybean. To identify single-nucleotide polymorphisms (SNPs) between a pair of G. latifolia accessions that were resistant or susceptible to Sclerotinia sclerotiorum (Lib.) de Bary, reduced-representations of DNAs from each accession were sequenced. Approximately 30 % of the 36 million 100-nt reads produced from each of the two G. latifolia accessions aligned primarily to gene-rich euchromatic regions on the distal arms of G. max chromosomes. Because a genome sequence was not available for G. latifolia, the G. max genome sequence was used as a reference to identify 9,303 G. latifolia SNPs that aligned to unique positions in the G. max genome with at least 98 % identity and no insertions and deletions. To validate a subset of the SNPs, nine TaqMan and 384 GoldenGate allele-specific G. latifolia SNP assays were designed and analyzed in F-2 G. latifolia populations derived from G. latifolia plant introductions (PI) 559298 and 559300. All nine TaqMan markers and 91 % of the 291 polymorphic GoldenGate markers segregated in a 1:2:1 ratio. Genetic linkage maps were assembled for G. latifolia, nine of which were uninterrupted and nearly collinear with the homoeologous G. max chromosomes. These results made use of a heterologous reference genome sequence to identify more than 9,000 informative high-quality SNPs for G. latifolia, a subset of which was used to generate the first genetic maps for any perennial Glycine species. C1 [Chang, Sungyul; Hartman, Glen L.; Lambert, Kris N.; Hobbs, Houston A.; Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Hartman, Glen L.; Singh, Ram J.; Domier, Leslie L.] ARS, USDA, Urbana, IL 61801 USA. RP Domier, LL (reprint author), ARS, USDA, Urbana, IL 61801 USA. EM leslie.domier@ars.usda.gov FU National Sclerotinia Initiative; United States Department of Agriculture-Agricultural Research Service FX This study was supported by funding from the National Sclerotinia Initiative and the United States Department of Agriculture-Agricultural Research Service. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA or the University of Illinois and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 84 TC 5 Z9 5 U1 0 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUN PY 2013 VL 126 IS 6 BP 1627 EP 1638 DI 10.1007/s00122-013-2079-8 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 151RM UT WOS:000319477900018 PM 23494395 ER PT J AU Wan, XF Barnett, JL Cunningham, F Chen, S Yang, GH Nash, S Long, LP Ford, L Blackmon, S Zhang, Y Hanson, L He, Q AF Wan, Xiu-Feng Barnett, J. Lamar Cunningham, Fred Chen, Si Yang, Guohua Nash, Shannon Long, Li-Ping Ford, Lorelei Blackmon, Sherry Zhang, Yan Hanson, Larry He, Qiang TI Detection of African swine fever virus-like sequences in ponds in the Mississippi Delta through metagenomic sequencing SO VIRUS GENES LA English DT Article DE African swine fever virus; Catfish pond; Freshwater; Metagenomics; Mississippi Delta; Asfarviridae ID DRINKING-WATER; OUTBREAK AB Metagenomic characterization of water virome was performed in four Mississippi catfish ponds. Although differing considerably from African swine fever virus (ASFV), 48 of 446,100 sequences from 12 samples were similar enough to indicate that they represent new members in the family Asfarviridae. At present, ASFV is the only member of Asfarviridae, and this study presents the first indication of a similar virus in North America. At this point, there is no indication that the identified virus(es) pose a threat to human or animal health, and further study is needed to characterize their potential risks to both public health and agricultural development. C1 [Wan, Xiu-Feng; Barnett, J. Lamar; Yang, Guohua; Nash, Shannon; Long, Li-Ping; Ford, Lorelei; Blackmon, Sherry; Hanson, Larry] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Cunningham, Fred] USDA APHIS WS, Natl Wildlife Res Ctr, Mississippi Field Stn, Mississippi State, MS USA. [Chen, Si; Zhang, Yan; He, Qiang] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wanhenry@yahoo.com RI He, Qiang/G-9061-2011; Chen, Si/C-4587-2015; Zhang, Yan/L-1738-2015 OI He, Qiang/0000-0002-7155-6474; FU USDA APHIS NWRC Award [1174280807CA 12030306]; NIH [RC1AI086830] FX We thank Mary Duke and Dr. Brian Scheffler at USDA-ARS, Mid-South Area Genomics Laboratory, Stoneville, MS for genomic sequencing. This Project was supported by USDA APHIS NWRC Award 1174280807CA 12030306 and NIH RC1AI086830 to XFW. NR 16 TC 7 Z9 7 U1 1 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD JUN PY 2013 VL 46 IS 3 BP 441 EP 446 DI 10.1007/s11262-013-0878-2 PG 6 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 154MS UT WOS:000319680500006 PM 23338931 ER PT J AU Alabi, OJ Poojari, S Sarver, K Martin, RR Naidu, RA AF Alabi, Olufemi J. Poojari, Sudarsana Sarver, Kara Martin, Robert R. Naidu, Rayapati A. TI Complete genome sequence analysis of an American isolate of Grapevine virus E SO VIRUS GENES LA English DT Article DE Grapevine virus E; Vitis sp.; Vitiviruses; Betaflexiviridae ID RNA HELICASE AB The complete genome sequence of Grapevine virus E (GVE) collected from a red-berried wine grape cultivar (Cabernet Sauvignon) in Washington State was determined. The 7,568 nucleotide long genome of GVE is more similar in sequence identity with a GVE isolate from a wine grape cv. Shiraz from South Africa when compared with an isolate from "Aki Queen" grape from Japan. Like GVE isolates from South Africa and Japan, the Washington isolate encodes five open reading frames (ORFs) and the overall genome organization is identical among these isolates. In addition to AlkB domain, a DExD domain, belonging to the DEAD-like helicases superfamily, was present upstream of the helicase domain in the replicase ORF of the virus. C1 [Alabi, Olufemi J.; Poojari, Sudarsana; Naidu, Rayapati A.] Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. [Sarver, Kara; Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Naidu, RA (reprint author), Washington State Univ, Ctr Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. EM naidu.rayapati@wsu.edu OI Alabi, Olufemi/0000-0002-2471-7052 FU Washington State University's Agricultural Research Center in the College of Agricultural, Human, and Natural Resource Sciences; Wine Advisory Committee of the Washington Wine Commission; United States Department of Agriculture (USDA) Agricultural Research Services Northwest Center for Small Fruits Research; USDA-National Institute of Food and Agriculture Specialty Crop Research Initiative [2009-51181-06027] FX This work was supported, in part, by Washington State University's Agricultural Research Center in the College of Agricultural, Human, and Natural Resource Sciences, Wine Advisory Committee of the Washington Wine Commission, United States Department of Agriculture (USDA) Agricultural Research Services Northwest Center for Small Fruits Research, and USDA-National Institute of Food and Agriculture Specialty Crop Research Initiative (Award Number 2009-51181-06027). PPNS number 0605, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center Project Number WNPO 0616, Washington State University, Pullman 99164-6240. NR 10 TC 2 Z9 4 U1 0 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 J9 VIRUS GENES JI Virus Genes PD JUN PY 2013 VL 46 IS 3 BP 563 EP 566 DI 10.1007/s11262-012-0872-0 PG 4 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA 154MS UT WOS:000319680500024 PM 23296875 ER PT J AU Gonzalez, VH Griswold, TL AF Gonzalez, Victor H. Griswold, Terry L. TI Wool carder bees of the genus Anthidium in the Western Hemisphere (Hymenoptera: Megachilidae): diversity, host plant associations, phylogeny, and biogeography SO ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Review DE Anthophila; Anthidiini; Apoidea; glandular trichomes; nototribic flowers; morphology; species hypotheses; systematics ID TWIG-NESTING HYMENOPTERA; NORTH-AMERICA; PARASITE ASSOCIATIONS; CRESSON HYMENOPTERA; GALAPAGOS-ISLANDS; SOLITARY BEE; MANICATUM; APOIDEA; CALLANTHIDIUM; HALICTIDAE AB Bees are among the most important pollinators of flowering plants in most ecosystems. Recent concerns about population decline worldwide have highlighted the urgent societal need for species-level systematic work that facilitates assessments of the status of pollinators and pollination services. This is a comprehensive, broadly comparative study on the diversity, biology, biogeography, and evolution of Anthidium Fabricius, 1804, one of the most diverse megachilid genera, containing more than 160 species worldwide. Herein, the Western Hemisphere species are revised. All 92 recognized species, including the two adventive species Anthidium oblongatum (Illiger, 1806) and Anthidium manicatum (Linnaeus, 1758), are described and illustrated. A neotype for Anthidium emarginatum (Say, 1824) and lectotypes for 16 names are designated; five names are relegated to synonymy, three names are revalidated, previously unknown males of three species are described, and 21 new species are proposed. Identification keys as well as information on the distribution, seasonality, nesting biology, and host plants are provided. The relationships of the Anthidium subgenera and all Western Hemisphere species are explored using a cladistic analysis based on adult external morphological characters. The subgenus CallanthidiumCockerell, 1925, renders Anthidiums.s. paraphylectic in the analysis, and is here synonymized. The resulting phylogenetic hypothesis is used to examine possible biogeographical patterns, origins of the Western Hemisphere fauna, and the evolution of morphological traits associated with foraging for pollen from nototribic flowers and exudates from glandular trichomes. To facilitate the transfer of knowledge to non-specialists, some digital outputs and web-based products, including a geo-referenced specimen database consisting of more than 20000 records, species pages, and interactive digital keys, were also developed during this study.(c) 2013 The Linnean Society of London C1 [Gonzalez, Victor H.; Griswold, Terry L.] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Gonzalez, VH (reprint author), Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. EM victorgonzab@gmail.com RI Gonzalez, Victor/B-4072-2015 FU National Science Foundation [DEB-0742998, DBI-0956388] FX We are greatly indebted to each of the curators, collection managers, and staff from the collections that we visited, or from which we borrowed specimens ( indicated in Material and methods). We also appreciate the time and effort of the many collectors that made this study possible. We thank Amy Comfort de Gonzalez, Molly Rightmyer, Kim Huntzinger, John Ascher, Laurence Packer, Frank Parker, Antonio Aguiar, and anonymous reviewers for their comments and suggestions that improved this work; Roxana Arauco for her help taking some of the photographs of the female clypeus; Jon Koch for the global distribution map of A. manicatum; Jim Cane for allowing us to use his photograph of A. manicatum; Nydia L. Vitale for her comments on the plant hosts of A. friesei; and Harold Ikerd, Kim Huntzinger, Emily Bourne, Camden Hunt, Skyler Burrows, and Jon Koch for entering label data into the US National Pollination Insects Database, and for producing the maps. This study was supported in part by National Science Foundation grants DEB-0742998 and DBI-0956388. NR 198 TC 7 Z9 7 U1 1 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4082 EI 1096-3642 J9 ZOOL J LINN SOC-LOND JI Zool. J. Linn. Soc. PD JUN PY 2013 VL 168 IS 2 SI SI BP 221 EP 425 DI 10.1111/zoj.12017 PG 205 WC Zoology SC Zoology GA 153QB UT WOS:000319616200001 ER PT J AU Stark, JC Love, SL King, BA Marshall, JM Bohl, WH Salaiz, T AF Stark, J. C. Love, S. L. King, B. A. Marshall, J. M. Bohl, W. H. Salaiz, T. TI Potato Cultivar Response to Seasonal Drought Patterns SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Irrigation; Water stress; Water use efficiency ID FRESH-MARKET; WATER-STRESS; HIGH-YIELD; RUSSET; QUALITY; GROWTH; VARIETY; CONDUCTANCE; IRRIGATION; ADAPTATION AB The ability to minimize potato yield and quality losses due to drought can be greatly improved by understanding the relative responses of different cultivars to seasonal variations in water supply. To address this need, we initiated a 2 year field experiment to determine the responses of the six potato cultivars to different seasonal drought patterns, including 1) full season irrigation at 100 % ET, 2) irrigation at 100 % ET terminated during late bulking , 3) full season irrigation at 70 % ET , 4) irrigation at 70 % ET terminated during late bulking , and 5) a gradual reduction in irrigation from 100 % ET during tuber initiation through early bulking, to 70 % ET during mid-bulking, and 50 % ET through late bulking. GemStar Russet and Ranger Russet, two medium-late maturing cultivars, generally produced the highest yields across the range of drought treatments, but both were fairly sensitive to changes in drought severity. Alturas, a late maturing cultivar, produced relatively high yields with full irrigation, but exhibited the greatest sensitivity to increasing drought severity, particularly when severe late-season water deficits were imposed. Yields for the early maturing cultivar Russet Norkotah were relatively low overall, but it was the least sensitive to changes in drought severity, particularly when late season drought was imposed. Russet Burbank produced comparatively high total yields across the range of drought treatments, but U.S. No. 1 yields were substantially reduced by each seasonal drought pattern. However, it was less sensitive to changes in drought severity than GemStar Russet, Ranger Russet and Alturas. Total and U.S. No. 1 yields for Summit Russet were low for each drought treatment and it exhibited intermediate sensitivity to changes in drought severity. GemStar Russet had the highest water use efficiency based on U.S. No. 1 yield. C1 [Stark, J. C.; Marshall, J. M.] Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. [Love, S. L.; Salaiz, T.] Univ Idaho, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. [King, B. A.] USDA ARS NWISRL, Kimberly, ID 83341 USA. [Bohl, W. H.] Univ Idaho, Bingham Co Extens, Blackfoot, ID 83221 USA. RP Stark, JC (reprint author), Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. EM jstark@uidaho.edu FU USDA RMA/RED Partnership Program FX Financial support for this research was provided by a grant from the USDA RMA/RED Partnership Program. We also thank Peggy Bain, Mel Chappell and Bill Price for their technical assistance in the preparation of this manuscript. NR 30 TC 4 Z9 4 U1 3 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2013 VL 90 IS 3 BP 207 EP 216 DI 10.1007/s12230-012-9285-9 PG 10 WC Agronomy SC Agriculture GA 149UA UT WOS:000319345700001 ER PT J AU Seefeldt, SS Boydston, RA Kaspari, PN Zhang, MC Carr, E Smeenk, J Barnes, DL AF Seefeldt, Steven S. Boydston, Rick A. Kaspari, Phil N. Zhang, Mingchu Carr, Erin Smeenk, Jeff Barnes, David L. TI Aminopyralid Residue Impacts on Potatoes and Weeds SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Bioassay; Carryover; Dose-response ID VEGETABLE CROPS; SOIL; PERSISTENCE; HERBICIDES; WINTER AB Aminopyralid is used in Alaska to control certain invasive weed species; however it appears to have an extended soil half-life in interior Alaska resulting in carry-over injury in potatoes. Field studies at three experiment stations in Delta Junction, Fairbanks, and Palmer, Alaska were established to determine the dose-response of weeds and above and below ground potato growth to soil-applied aminopyralid (0, 8, 15, 31, 62, and 123 g ae ha(-1)) . Both prostrate knotweed and narrowleaf hawksbeard were susceptible to aminopyralid. At Delta Junction and Fairbanks, visual injury of potatoes greater than 25 % was observed at 15 g ae ha(-1) aminopyralid, whereas at Palmer visual injury was greater than 40 % at 8 g ae ha(-1), the lowest rate tested. Potato tuber production was reduced by aminopyralid at rates of 15 g ae ha(-1) and above at both Delta Junction and Palmer. Sub-samples of potato tubers from Delta Junction and Palmer were analyzed for aminopyralid content and grown out to determine if aminopyralid in tubers would reduce subsequent growth. The aminopyralid concentration in potato tubers increased with increasing field application rates, with 30 ppb extracted from tubers grown at the highest application rate (123 g ae ha(-1)). All plants grown from daughter tubers except from control plots in Palmer exhibited injury symptoms. The number of emerged shoots, and shoot height decreased with increasing aminopyralid concentrations in the tuber, with injury rates greater than 70 % at 8 g ae ha(-1). C1 [Seefeldt, Steven S.] Univ Alaska Fairbanks, Cooperat Extens Serv Tanana Dist, Fairbanks, AK 99775 USA. [Boydston, Rick A.] ARS, USDA, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Kaspari, Phil N.] Univ Alaska Fairbanks, Cooperat Extens Serv Delta Dist, Delta Junction, AK 99737 USA. [Zhang, Mingchu; Carr, Erin] Univ Alaska Fairbanks, Dept High Latitude Agr, Fairbanks, AK 99775 USA. [Smeenk, Jeff] Palmer Soil & Water Dist, Palmer, AK 99645 USA. [Barnes, David L.] Univ Alaska Fairbanks, Dept Civil & Environm Engn, Fairbanks, AK 99775 USA. RP Seefeldt, SS (reprint author), Univ Alaska Fairbanks, Cooperat Extens Serv Tanana Dist, Fairbanks, AK 99775 USA. EM ssseefeldt@alaska.edu NR 14 TC 3 Z9 3 U1 2 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2013 VL 90 IS 3 BP 239 EP 244 DI 10.1007/s12230-012-9298-4 PG 6 WC Agronomy SC Agriculture GA 149UA UT WOS:000319345700004 ER PT J AU Larkin, RP Tavantzis, S AF Larkin, Robert P. Tavantzis, Stellos TI Use of Biocontrol Organisms and Compost Amendments for Improved Control of Soilborne Diseases and Increased Potato Production SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Rhizoctonia solani; Streptomyces scabiei; Disease suppression; Hypovirulent; Tuber yield; Black scurf; Common scab; Stem and stolon canker; Biological control; Integrated pest management; Bacillus subtilis ID SOIL MICROBIAL COMMUNITIES; BIOLOGICAL-CONTROL; RHIZOCTONIA-SOLANI; PLANT-DISEASES; GREEN MANURES; FUNGAL DISEASES; BLACK SCURF; DAMPING-OFF; SUPPRESSION; AGENTS AB Soilborne potato diseases are persistent problems in potato production and alternative management practices are needed. In this research, biocontrol agents (Bacillus subtilis GB03 and Rhizoctonia solani hypovirulent isolate Rhs1A1) and compost amendments (from different source material), were evaluated alone and in combination, for their potential to reduce soilborne diseases and increase tuber yield over three field seasons in Maine. Both biocontrol organisms reduced multiple soilborne diseases, stem and stolon canker by 20-38 %, black scurf by 30-58 %, and common scab by 10-34 % relative to the nontreated control treatment, and the combination treatment of both biocontrol organisms together provided nominally better control than individual treatments. However, biocontrol treatments had no direct effect on tuber yield. Compost amendments from different sources all increased total and marketable tuber yield substantially (11-37 % and 17-51 %, respectively) relative to nontreated controls. However, except for some reduction of Rhizoctonia canker, compost amendments did not reduce soilborne diseases (black scurf and commons scab) in any year, and resulted in increased levels of common scab in some years (20-45 % increase). The combination compost-biocontrol treatment, although did not perform significantly better than individual component treatments, still provided indications of combined beneficial effects from both component treatments. This research demonstrated the usefulness of these approaches and combinations as additional options for reduction of soilborne diseases and increased tuber yield and can be implemented for enhanced sustainability and productivity in potato production systems. C1 [Larkin, Robert P.] USDA ARS, New England Plant Soil & Water Lab, Orono, ME 04469 USA. [Tavantzis, Stellos] Univ Maine, Sch Biol & Ecol, Orono, ME 04469 USA. RP Larkin, RP (reprint author), USDA ARS, New England Plant Soil & Water Lab, Orono, ME 04469 USA. EM bob.larkin@ars.usda.gov NR 42 TC 7 Z9 7 U1 4 U2 41 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2013 VL 90 IS 3 BP 261 EP 270 DI 10.1007/s12230-013-9301-8 PG 10 WC Agronomy SC Agriculture GA 149UA UT WOS:000319345700007 ER PT J AU Thornton, MK Lee, J John, R Olsen, NL Navarre, DA AF Thornton, Michael K. Lee, Jungmin John, Robin Olsen, Nora L. Navarre, Duroy A. TI Influence of Growth Regulators on Plant Growth, Yield, and Skin Color of Specialty Potatoes SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE 2,4-dichlorophenoxyacetic acid; Naphthalene acetic acid; Lysophosphatidylethanolamine; Plant growth regulators ID SOLANUM-TUBEROSUM-L; 2,4-DICHLOROPHENOXYACETIC ACID; ANTHOCYANIN ACCUMULATION; ETHYLENE PRODUCTION; PERIDERM COLOR; RED; LYSOPHOSPHATIDYLETHANOLAMINE; VARIETIES; QUALITY; 2,4-D AB 2,4-D has been used since the 1950's to enhance color in red-skinned potatoes, but there is little research on the potential use of other plant growth regulators to improve tuber skin color in the wide range of specialty potatoes now available on the market. Field trials conducted at Parma, ID in 2009 and 2010 evaluated the effect of foliar applications of 2,4-D, NAA, and LPE on plant height, foliar injury, tuber yield, size distribution, and skin color of six specialty potato cultivars. Skin color was rated visually and via HunterLab colorimeter at harvest, and again after storage (132 days in 2009 and 93 days in 2010) at 4 A degrees C and 95 % R.H. 2,4-D caused slight foliar injury and reduced tuber size in all cultivars. It also increased visual skin color rating in the red-skinned cultivars 'Red LaSoda' and 'TerraRosa' at harvest and after storage, and influenced incidence of pink coloration around the eyes of 'Yukon Gem' tubers, but did not affect color of blue/purple-skinned cultivars. NAA and LPE did not consistently influence plant growth, yield, tuber size or visual skin color in any cultivar. Colorimetric evaluations mostly agreed with the visual ratings, and indicated that storage significantly altered skin color in all cultivars, regardless of growth regulator treatment. C1 [Thornton, Michael K.; John, Robin] Univ Idaho, Dept Plant Soil & Entomol Sci, Parma Res & Extens Ctr, Parma, ID 83660 USA. [Lee, Jungmin] USDA ARS, Hort Crops Res Unit HCRU Worksite, Parma, ID 83660 USA. [Olsen, Nora L.] Univ Idaho, Dept Plant Soil & Entomol Sci, Kimberly Res & Extens Ctr, Kimberly, ID 83341 USA. [Navarre, Duroy A.] USDA ARS, Vegetable & Forage Crops Res Lab, Prosser, WA 99350 USA. RP Thornton, MK (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Parma Res & Extens Ctr, Parma, ID 83660 USA. EM miket@uidaho.edu RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 FU Idaho Potato Commission; USDA Potato Research program; USDA-Agricultural Research Service (ARS) CRIS [5358-21000-041-00D] FX This research was performed as part of the thesis of Robin John. We thank William Buhrig, Christopher Rennaker, and Lynn Woodell for their help in collecting data for these studies, and the Idaho Potato Commission, the USDA Potato Research program, and USDA-Agricultural Research Service (ARS) CRIS number 5358-21000-041-00D for financial support. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 31 TC 6 Z9 7 U1 0 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2013 VL 90 IS 3 BP 271 EP 283 DI 10.1007/s12230-013-9302-7 PG 13 WC Agronomy SC Agriculture GA 149UA UT WOS:000319345700008 ER PT J AU Cotta, MA Whitehead, TR Falsen, E Moore, E Lawson, PA AF Cotta, Michael A. Whitehead, Terence R. Falsen, Enevold Moore, Edward Lawson, Paul A. TI Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit (vol 103, pg 89, 2013) SO ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY LA English DT Correction DE Enterococci; 16S rRNA; Phylogeny; Taxonomy; Manure; Swine ID SEQUENCE-ANALYSIS; BACTERIA; IDENTIFICATION; ENUMERATION; POPULATIONS; MEDIA; FECES; ACID AB A polyphasic taxonomic study using morphological, biochemical, chemotaxonomic and molecular genetic methods was performed on six strains of an unknown Gram-positive, nonspore-forming, facultative anaerobic coccus-shaped bacterium isolated from a swine-manure storage pit. On the basis of 16S rRNA, RNA polymerase-subunit (rpoA), and the 60-kilodalton chaperonin (cpn60) gene sequence analyses, it was shown that all the isolates were enterococci but formed two separate lines of descent. Pairwise 16S rRNA sequence comparisons demonstrated that the two novel organisms were most closely related to each other (97.9 %) and to Enterococcus aquimarinus (97.8 %). Both organisms contained major amounts of C-16:0, C-16:1 omega 7c, and C-18:1 omega 7c/12t/9t as the major cellular fatty acids. Based on biochemical, chemotaxonomic, and phylogenetic evidence, the names Enterococcus lemanii sp. nov. (type strain PC32(T) = CCUG 61260(T) = NRRL B-59661(T)) and Enterococcus eurekensis sp. nov. (type strain PC4B(T) = CCUG 61259(T) = NRRL B-59662(T)) are proposed for the hitherto undescribed species. C1 [Cotta, Michael A.; Whitehead, Terence R.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Falsen, Enevold; Moore, Edward] Univ Gothenburg, Dept Clin Bacteriol, S-41346 Gothenburg, Sweden. [Lawson, Paul A.] Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA. [Lawson, Paul A.] Univ Oklahoma, Ecol & Evolutionary Biol Program, Norman, OK 73019 USA. RP Whitehead, TR (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Terry.Whitehead@ars.usda.gov OI Cotta, Michael/0000-0003-4565-7754 NR 40 TC 0 Z9 0 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0003-6072 J9 ANTON LEEUW INT J G JI Antonie Van Leeuwenhoek PD JUN PY 2013 VL 103 IS 6 BP 1409 EP 1418 DI 10.1007/s10482-013-9908-2 PG 10 WC Microbiology SC Microbiology GA 148TA UT WOS:000319269200022 PM 23592176 ER PT J AU Hagler, JR Blackmer, F Krugner, R Groves, RL Morse, JG Johnson, MW AF Hagler, James R. Blackmer, Felisa Krugner, Rodrigo Groves, Russell L. Morse, J. G. Johnson, Marshall W. TI Gut content examination of the citrus predator assemblage for the presence of Homalodisca vitripennis remains SO BIOCONTROL LA English DT Article DE Glassy-winged sharpshooter; Conservation biological control; ELISA; Generalist predators; Molecular gut content analysis; PCR; Predator-prey interactions; Spiders ID GLASSY-WINGED SHARPSHOOTER; POLYMERASE-CHAIN-REACTION; MONOCLONAL-ANTIBODY; ALTERNATIVE PREY; INVERTEBRATE PREDATORS; GONATOCERUS-ASHMEADI; COAGULATA HEMIPTERA; XYLELLA-FASTIDIOSA; EGG PARASITOIDS; LYGUS-HESPERUS AB A two-year study was conducted in a citrus orchard, Citrus sinensis L., to determine frequency of predation on glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar). A total of 1,578 arthropod predators, representing 18 taxa, were collected and assayed for the presence of GWSS egg protein by an enzyme-linked immunosorbent assay using a Homalodisca-species and egg-specific monoclonal antibody and then by polymerase chain reaction using a H. vitripennis-specific DNA marker. The gut content analyses revealed the presence of GWSS remains in the gut of 2.28 % of the total arthropod predator population, with 3.09 % of the spiders and 0.59 % of the insect predators testing positive. Moreover, a comparison of the two assays indicated that they were not equally effective at detecting GWSS remains in predator guts. Low frequencies of GWSS detection in the gut of predators indicated that GWSS are not a primary prey and that predators may contribute little to suppression of this pest in citrus. C1 [Hagler, James R.; Blackmer, Felisa] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Krugner, Rodrigo] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. [Groves, Russell L.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Morse, J. G.; Johnson, Marshall W.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Hagler, JR (reprint author), ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. EM James.Hagler@ars.usda.gov FU California Department of Food and Agriculture; University of California's Pierce's Disease Grant Program; USDA-ARS FX We would like to thank Scott Machtley and Arnel P. Flores for their excellent technical support. This work was funded by the California Department of Food and Agriculture, the University of California's Pierce's Disease Grant Program, and the USDA-ARS. NR 45 TC 4 Z9 6 U1 6 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 J9 BIOCONTROL JI Biocontrol PD JUN PY 2013 VL 58 IS 3 BP 341 EP 349 DI 10.1007/s10526-012-9489-4 PG 9 WC Entomology SC Entomology GA 149UN UT WOS:000319347000006 ER PT J AU Choate, BA Lundgren, JG AF Choate, Beth A. Lundgren, Jonathan G. TI Why eat extrafloral nectar? Understanding food selection by Coleomegilla maculata (Coleoptera: Coccinellidae) SO BIOCONTROL LA English DT Article DE Omnivore; Coleoptera; Coccinellidae; Extrafloral nectar; Lady beetle; Sugar feeding ID POTATO BEETLE COLEOPTERA; LADY BEETLE; NEUROPTERA-CHRYSOPIDAE; OLFACTORY REACTIONS; BIOLOGICAL-CONTROL; NATURAL ENEMIES; PREY; PLANT; CHRYSOMELIDAE; PREDATION AB Methods of increasing predator abundance within a habitat include the incorporation of non-prey food items, yet the influence of this on predation intensity toward herbivores remains unknown. In order to gain an understanding of nectar feeding in the predaceous beetle, Coleomegilla maculata (DeGeer), laboratory studies were conducted evaluating prey consumption in the presence of extrafloral nectaries. The physiology of beetles with access to prey only and a mixed diet were compared. To elucidate results of beetle physiology, Y-tube olfactometer studies were conducted and preferences between food types evaluated. Coleomegilla maculata females consumed 9 % fewer aphids when nectar was available. Lipid and glycogen content, as well as oocyte volume were not increased upon consumption of a mixed diet. Evaluation of predator behavior when offered both food resources together and separately demonstrated that extrafloral nectaries are attractive. C1 [Choate, Beth A.] Allegheny Coll, Dept Environm Sci, Meadville, PA 16335 USA. [Choate, Beth A.; Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Choate, BA (reprint author), Allegheny Coll, Dept Environm Sci, 520 N Main St, Meadville, PA 16335 USA. EM bchoate@allegheny.edu; Jonathan.Lundgren@ars.usda.gov FU Binational Agricultural Research and Development Fund FX Invaluable advice was provided by Michael Seagraves with regards to laboratory feeding assays. Thank you to Janet Fergen, Chloe Kruse, Ryan Schmidt, Mike Bredeson, and Mallory and Meagan Wentzel for their assistance in caring for insect and plant resources, as well as execution of the discussed experiments. Thank you to Ted Evans and Michael Seagraves for reading an earlier version of this manuscript. This work was supported by the Binational Agricultural Research and Development Fund. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 40 TC 9 Z9 9 U1 7 U2 57 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 J9 BIOCONTROL JI Biocontrol PD JUN PY 2013 VL 58 IS 3 BP 359 EP 367 DI 10.1007/s10526-012-9501-z PG 9 WC Entomology SC Entomology GA 149UN UT WOS:000319347000008 ER PT J AU DePiper, GS Higgins, N Lipton, DW Stocking, A AF DePiper, Geret S. Higgins, Nathaniel Lipton, Douglas W. Stocking, Andrew TI Auction Design, Incentives, and Buying Back Maryland and Virginia Crab Licenses SO CANADIAN JOURNAL OF AGRICULTURAL ECONOMICS-REVUE CANADIENNE D AGROECONOMIE LA English DT Article ID RESERVE PRICES; BUYBACK PROGRAMS; FISHERIES; SUBSIDIES; MARKET; POLICY AB Fisheries managers use buybacks to reduce fleet capacity, conserve fish stocks, and accomplish other goals. In 2009, Maryland and Virginia conducted auctions to buy back commercial fishing licenses. The auctions in both States had similar timing, objectives, and target populations. The divergent designs of the auctions, however, provide a case study with which to investigate effective buyback practices. We compare and contrast the market designs used by Maryland and Virginia, and describe how those differing designs influenced the respective outcomes. Les gestionnaires des peches recourent aux rachats de permis pour reduire la capacite des flottilles de peche, proteger les stocks de poissons et realiser divers objectifs. En 2009, les Etats du Maryland et de la Virginie ont organise des ventes aux encheres pour racheter des permis de peche commerciale. Le moment choisi, les objectifs et les populations cibles de ces deux Etats etaient similaires. Toutefois, les conceptions des encheres etaient differentes et offraient l'occasion d'examiner les pratiques de rachat efficaces. Nous faisons ressortir les similitudes et les differences des conceptions du marche utilisees par le Maryland et la Virginie, et nous decrivons de quelle facon ces conceptions ont influence les resultats respectifs. C1 [DePiper, Geret S.] Natl Marine Fisheries Serv, US Dept Commerce, Woods Hole, MA 02543 USA. [Higgins, Nathaniel] Econ Res Serv, USDA, Washington, DC 20250 USA. [Lipton, Douglas W.] Univ Maryland, Dept Agr & Resource Econ, College Pk, MD 20742 USA. [Stocking, Andrew] Congress Budget Off, Washington, DC 20515 USA. RP DePiper, GS (reprint author), Natl Marine Fisheries Serv, US Dept Commerce, 166 Water St, Woods Hole, MA 02543 USA. EM geret.depiper@noaa.gov; nhiggins@ers.usda.gov; dlipton@arec.umd.edu; astocking@cbo.gov NR 49 TC 3 Z9 3 U1 3 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0008-3976 J9 CAN J AGR ECON JI Can. J. Agric. Econ.-Rev. Can. Agroecon. PD JUN PY 2013 VL 61 IS 2 SI SI BP 353 EP 370 DI 10.1111/cjag.12005 PG 18 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 149UM UT WOS:000319346900011 ER PT J AU Edwards, JV Prevost, N Sethumadhavan, K Ullah, A Condon, B AF Edwards, J. Vincent Prevost, Nicolette Sethumadhavan, Kandan Ullah, Abul Condon, Brian TI Peptide conjugated cellulose nanocrystals with sensitive human neutrophil elastase sensor activity SO CELLULOSE LA English DT Article DE Human neutrophil elastase; Biosensor; Cotton cellulose nanocrystals; Peptide; Dialysis cellulose membrane; Chronic wounds ID CHRONIC VENOUS ULCERS; WOUND FLUID; REINFORCED NANOCOMPOSITES; MATRIX; COTTON; DEGRADATION; INHIBITION; DRESSINGS; PROTEASES; WHISKERS AB In chronic wounds, elevated human neutrophil elastase (HNE) is a destructive protease that has been proposed as a biomarker. Numerous wound dressing designs have been introduced in an effort to lower HNE levels. The clinical detection of HNE as a point of care biomarker or an in situ colorimetric adjuvant to chronic wound dressings presents potential advantages in the management of chronic wounds. A colorimetric approach to the detection of HNE using peptide conjugated cotton cellulose nanocrystals (CCN) is reported here. For this purpose a HNE tripeptide substrate, n-Succinyl-Alanine-Alanine-Valine-para-nitroanilide (Suc-Ala-Ala-Val-pNA), was covalently attached to glycine esterified CCN and compared with a similar tetrapeptide analog for colorimetric HNE sensor activity. Visible HNE activity was significantly higher on CCN tripeptide conjugates when compared with similar analogs synthesized on paper. Upon enzymatic release of para-nitroaniline (pNA) from the Glycine-CCN conjugate of succinyl-Ala-Ala-Val-pNA, amplification of the colorimetric response from pNA with reactive dyes enhanced visible absorption of the chromogen. Two color amplifying dyes that react with pNA were compared for their ability to enhance the visual sensor response to HNE activity. The colorimetric detection of HNE with CCN tripeptide conjugates was sensitive at HNE levels previously reported in chronic wound fluid (0.05 U/mL HNE). The HNE sensor and the chromogen amplifying dyes were interfaced with 50 and 10 kD dialysis cellulose membranes (DCM) to model filtration of HNE and chromogen (pNA) from a model wound dressing surface before and after sensor reactivity. The detection sensitivity to HNE activity was assessed with the CCN-tripeptide conjugate interfaced at the DCM surface distal and proximal to a dressing surface. The HNE sensor interfaced proximal to the dressing surface was most efficient with 10 kD membrane filtration of pNA and subsequent reaction with amplifying dyes. When interfaced with the 10 kD cellulose membrane, elastase sensor activity remained sensitive to 0.05 U/mL HNE. The nanocellulose surface properties, performance and design issues of the biosensor approach are discussed. C1 [Edwards, J. Vincent; Prevost, Nicolette; Sethumadhavan, Kandan; Ullah, Abul; Condon, Brian] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Edwards, JV (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM vince.edwards@ars.usda.gov NR 66 TC 23 Z9 24 U1 3 U2 53 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 J9 CELLULOSE JI Cellulose PD JUN PY 2013 VL 20 IS 3 BP 1223 EP 1235 DI 10.1007/s10570-013-9901-y PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA 143MB UT WOS:000318870500023 ER PT J AU Stabel, JR Waters, WR Bannantine, JP Palmer, MV AF Stabel, J. R. Waters, W. R. Bannantine, J. P. Palmer, M. V. TI Disparate Host Immunity to Mycobacterium avium subsp paratuberculosis Antigens in Calves Inoculated with M. avium subsp paratuberculosis, M. avium subsp avium, M. kansasii, and M. bovis SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID T-CELLS; CULTURE FILTRATE; NEONATAL CALVES; JOHNES-DISEASE; FUSION PROTEIN; CATTLE; RESPONSES; TUBERCULOSIS; CD26; INFECTIONS AB The cross-reactivity of mycobacterial antigens in immune-based diagnostic assays has been a major concern and a criticism of the current tests that are used for the detection of paratuberculosis. In the present study, Mycobacterium avium subsp. paratuberculosis recombinant proteins were evaluated for antigenic specificity compared to a whole-cell sonicate preparation (MPS). Measures of cell-mediated immunity to M. avium subsp. paratuberculosis antigens were compared in calves inoculated with live M. avium subsp. paratuberculosis, M. avium subsp. avium (M. avium), Mycobacterium kansasii, or Mycobacterium bovis. Gamma interferon (IFN-gamma) responses to MPS were observed in all calves that were exposed to mycobacteria compared to control calves at 4 months postinfection. Pooled recombinant M. avium subsp. paratuberculosis proteins also elicited nonspecific IFN-gamma responses in inoculated calves, with the exception of calves infected with M. bovis. M. avium subsp. paratuberculosis proteins failed to elicit antigen-specific responses for the majority of immune measures; however, the expression of CD25 and CD26 was upregulated on CD4, CD8, gamma/delta (gamma delta) T, and B cells for the calves that were inoculated with either M. avium subsp. paratuberculosis or M. avium after antigen stimulation of the cells. Stimulation with MPS also resulted in the increased expression of CD26 on CD45RO(+) CD25(+) T cells from calves inoculated with M. avium subsp. paratuberculosis and M. avium. Although recombinant proteins failed to elicit specific responses for the calves inoculated with M. avium subsp. paratuberculosis, the differences in immune responses to M. avium subsp. paratuberculosis antigens were dependent upon mycobacterial exposure. The results demonstrated a close alignment in immune responses between calves inoculated with M. avium subsp. paratuberculosis and those inoculated with M. avium that were somewhat disparate from the responses in calves infected with M. bovis, suggesting that the biology of mycobacterial infection plays an important role in diagnosis. C1 [Stabel, J. R.; Waters, W. R.; Bannantine, J. P.; Palmer, M. V.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. RP Stabel, JR (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. EM judy.stabel@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 NR 40 TC 4 Z9 4 U1 2 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD JUN PY 2013 VL 20 IS 6 BP 848 EP 857 DI 10.1128/CVI.00051-13 PG 10 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 151HO UT WOS:000319451500011 PM 23554467 ER PT J AU Lyashchenko, KP Greenwald, R Esfandiari, J O'Brien, DJ Schmitt, SM Palmer, MV Waters, WR AF Lyashchenko, Konstantin P. Greenwald, Rena Esfandiari, Javan O'Brien, Daniel J. Schmitt, Stephen M. Palmer, Mitchell V. Waters, W. Ray TI Rapid Detection of Serum Antibody by Dual-Path Platform VetTB Assay in White-Tailed Deer Infected with Mycobacterium bovis SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID ODOCOILEUS-VIRGINIANUS; CERVUS-ELAPHUS; FALLOW DEER; TUBERCULOSIS; MICHIGAN; WILDLIFE; RESPONSES; TESTS; BCG; USA AB Bovine tuberculosis (TB) in cervids remains a significant problem affecting farmed herds and wild populations. Traditional skin testing has serious limitations in certain species, whereas emerging serological assays showed promising diagnostic performance. The recently developed immunochromatographic dual-path platform (DPP) VetTB assay has two antigen bands, T1 (MPB83 protein) and T2 (CFP10/ESAT-6 fusion protein), for antibody detection. We evaluated the diagnostic accuracy of this test by using serum samples collected from groups of white-tailed deer experimentally inoculated with Mycobacterium bovis, M. avium subsp. paratuberculosis, or M. bovis BCG Pasteur. In addition, we used serum samples from farmed white-tailed deer in herds with no history of TB, as well as from free-ranging white-tailed deer culled during field surveillance studies performed in Michigan known to have bovine TB in the wild deer population. The DPP VetTB assay detected antibody responses in 58.1% of experimentally infected animals within 8 to 16 weeks postinoculation and in 71.9% of naturally infected deer, resulting in an estimated test sensitivity of 65.1% and a specificity of 97.8%. The higher seroreactivity found in deer with naturally acquired M. bovis infection was associated with an increased frequency of antibody responses to the ESAT-6 and CFP10 proteins, resulting in a greater contribution of these antigens, in addition to MPB83, to the detection of seropositive animals, compared with experimental M. bovis infection. Deer experimentally inoculated with either M. avium subsp. paratuberculosis or M. bovis BCG Pasteur did not produce cross-reactive antibodies that could be detected by the DPP VetTB assay. The present findings demonstrate the relatively high diagnostic accuracy of the DPP VetTB test for white-tailed deer, especially in the detection of naturally infected animals. C1 [Lyashchenko, Konstantin P.; Greenwald, Rena; Esfandiari, Javan] Chembio Diagnost Syst Inc, Medford, NY USA. [O'Brien, Daniel J.; Schmitt, Stephen M.] Michigan Dept Nat Resources, Wildlife Dis Lab, Lansing, MI USA. [Palmer, Mitchell V.; Waters, W. Ray] Agr Res Serv, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Lyashchenko, KP (reprint author), Chembio Diagnost Syst Inc, Medford, NY USA. EM klyashchenko@chembio.com NR 26 TC 9 Z9 9 U1 1 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD JUN PY 2013 VL 20 IS 6 BP 907 EP 911 DI 10.1128/CVI.00120-13 PG 5 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 151HO UT WOS:000319451500018 PM 23595504 ER PT J AU Tucker, ML Yang, RH AF Tucker, Mark L. Yang, Ronghui TI A gene encoding a peptide with similarity to the plant IDA signaling peptide (AtIDA) is expressed most abundantly in the root-knot nematode (Meloidogyne incognita) soon after root infection SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Meloidogyne incognita; Root-knot nematode; Inflorescence deficient in abscission; IDA; IDL; MilDL1 accession KC237722; Secreted ID FLORAL ORGAN ABSCISSION; ESOPHAGEAL GLAND-CELLS; HETERODERA-GLYCINES; PARASITIC NEMATODES; INFLORESCENCE-DEFICIENT; ARABIDOPSIS-THALIANA; SECRETED PROTEINS; IDENTIFICATION; CLE; BETA-1,4-ENDOGLUCANASE AB Small peptides play important roles in intercellular signaling. Inflorescence deficient in abscission (ida) is an Arabidopsis mutant that does not abscise (shed) its flower petals. The IDA gene encodes a small, secreted peptide that putatively binds to two redundant receptor-like kinases (HAESA and HAESA-like2) that initiate a signal transduction pathway. We identified IDA-like (IDL) genes in the genomic sequence for Meloidogyne incognita and Meloidogyne hapla. No orthologous sequences were found in any other genus of nematodes. Transcript for both M. incognita and M. hapla IDLs were found in total RNA isolated from infected root systems of tomato, Solanum lycopersicurn. Five and three prime RACE of RNA from M. incognita infected tomato roots revealed a sequence of 392 nt that includes a poly (A) tail of 39 nt. The open reading frame encodes a 47 aa protein with a putative 25 aa N-terminal signal peptide. Expression of MilDL1 is very low in eggs and pre-parasitic J2 and rapidly increases in the first four days post inoculation (dpi) and then declines at approximately 14 dpi. A proposed role for the root-knot nematode IDL is discussed. Published by Elsevier Inc. C1 [Tucker, Mark L.; Yang, Ronghui] USDA, Soybean Genom & Improvement Lab, ARS, BARC West, Beltsville, MD 20705 USA. RP Tucker, ML (reprint author), USDA, Soybean Genom & Improvement Lab, ARS, BARC West, Bldg 006, Beltsville, MD 20705 USA. EM mark.tucker@ars.usda.gov FU USDA-ARS in-house FX This work was supported by USDA-ARS in-house funding to MLT. We wish to thank Valerie Williamson and Sylwia Fudali for M. hapla infected tomato roots, Susan Meyer and Reham Youssef for M. incognita and Carol Mass ler for technical expertise with RKN. NR 47 TC 4 Z9 4 U1 1 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 J9 EXP PARASITOL JI Exp. Parasitol. PD JUN PY 2013 VL 134 IS 2 BP 165 EP 170 DI 10.1016/j.exppara.2013.03.019 PG 6 WC Parasitology SC Parasitology GA 152PE UT WOS:000319542800007 PM 23538028 ER PT J AU Youssef, RM Kim, KH Haroon, SA Matthews, BF AF Youssef, Reham M. Kim, Kyung-Hwan Haroon, Sanaa A. Matthews, Benjamin F. TI Post-transcriptional gene silencing of the gene encoding aldolase from soybean cyst nematode by transformed soybean roots SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Gene silencing; RNA interference; Resistance; Plant-parasitic nematode; Soybean cyst nematode; Heterodera glycines; Soybean; Glycine max ID PLANT-PARASITIC NEMATODE; PLASMID CCDA PROTEIN; DOUBLE-STRANDED-RNA; HETERODERA-GLYCINES; CAENORHABDITIS-ELEGANS; MELOIDOGYNE-INCOGNITA; KNOT NEMATODE; FUNCTIONAL-ANALYSIS; INTERFERENCE; EXPRESSION AB Plant parasitic nematodes cause approximately 157 billion US dollars in losses worldwide annually. The soybean cyst nematode (SCN), Heterodera glycines, is responsible for an estimated one billion dollars in losses to the US farmer each year. A promising new approach for control of plant parasitic nematode control is gene silencing. We tested this approach by silencing the SCN gene HgALD, encoding fructose-1,6-diphosphate aldolase. This enzyme is important in the conversion of glucose into energy and may be especially important in actin-based motility during parasite invasion of its host. An RNAi construct targeted to silence HgALD was transformed into soybean roots of composite plants to examine its efficacy to reduce the development of females formed by SCN. The number of mature females on roots transformed with the RNAi construct designed to silence the HgALD gene was reduced by 58%. These results indicate that silencing the aldolase gene of SCN +can greatly decrease the number of female SCN reaching maturity, and it is a promising step towards broadening resistance of plants against plant-parasitic nematodes. Published by Elsevier Inc. C1 [Youssef, Reham M.; Matthews, Benjamin F.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Kim, Kyung-Hwan] Rural Dev Adm, Cell & Genet Div, Natl Inst Agr Biotechnol, Suwon 441100, South Korea. [Youssef, Reham M.; Haroon, Sanaa A.] Fayoum Univ, Dept Plant Protect, Fac Agr, Al Fayyum, Egypt. RP Matthews, BF (reprint author), USDA ARS, Soybean Genom & Improvement Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM ben.matthews@ars.usda.gov FU United Soybean Board; US-Egypt Science and Technology program FX DNA sequencing was performed by the Peter Van Berkum Laboratory, USDA-ARS Soybean Genomics & Improvement Laboratory. The authors thank Andrea Skantar, Peggy MacDonald and Eric Brewer for critical review of the manuscript and gratefully acknowledge support froth the United Soybean Board and the US-Egypt Science and Technology program. Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable. NR 46 TC 8 Z9 12 U1 1 U2 36 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 J9 EXP PARASITOL JI Exp. Parasitol. PD JUN PY 2013 VL 134 IS 2 BP 266 EP 274 DI 10.1016/j.exppara.2013.03.009 PG 9 WC Parasitology SC Parasitology GA 152PE UT WOS:000319542800023 PM 23541467 ER PT J AU Fuglie, K AF Fuglie, Keith TI WHY THE PESSIMISTS ARE WRONG SO IEEE SPECTRUM LA English DT Article C1 Econ Res Serv, USDA, Washington, DC USA. RP Fuglie, K (reprint author), Econ Res Serv, USDA, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0018-9235 J9 IEEE SPECTRUM JI IEEE Spectr. PD JUN PY 2013 VL 50 IS 6 BP 26 EP 32 PG 7 WC Engineering, Electrical & Electronic SC Engineering GA 154QY UT WOS:000319692200010 ER PT J AU Yang, ZH Grinchuk, V Smith, A Qin, BL Bohl, JA Sun, R Notari, L Zhang, ZY Sesaki, H Urban, JF Shea-Donohue, T Zhao, AP AF Yang, Zhonghan Grinchuk, Viktoriya Smith, Allen Qin, Bolin Bohl, Jennifer A. Sun, Rex Notari, Luigi Zhang, Zhongyan Sesaki, Hiromi Urban, Joseph F., Jr. Shea-Donohue, Terez Zhao, Aiping TI Parasitic Nematode-Induced Modulation of Body Weight and Associated Metabolic Dysfunction in Mouse Models of Obesity SO INFECTION AND IMMUNITY LA English DT Article ID DIET-INDUCED OBESITY; ALTERNATIVELY ACTIVATED MACROPHAGES; EPITHELIAL-CELL FUNCTION; INSULIN-RESISTANCE; MICE; INFLAMMATION; SENSITIVITY; PROTECTION; INFECTION; RECEPTOR AB Obesity is associated with a chronic low-grade inflammation characterized by increased levels of proinflammatory cytokines that are implicated in disrupted metabolic homeostasis. Parasitic nematode infection induces a polarized Th2 cytokine response and has been explored to treat autoimmune diseases. We investigated the effects of nematode infection against obesity and the associated metabolic dysfunction. Infection of RIP2-Opa1KO mice or C57BL/6 mice fed a high-fat diet (HFD) with Nippostrongylus brasiliensis decreased weight gain and was associated with improved glucose metabolism. Infection of obese mice fed the HFD reduced body weight and adipose tissue mass, ameliorated hepatic steatosis associated with a decreased expression of key lipogenic enzymes/mediators, and improved glucose metabolism, accompanied by changes in the profile of metabolic hormones. The infection resulted in a phenotypic change in adipose tissue macrophages that was characterized by upregulation of alternative activation markers. Interleukin-13 (IL-13) activation of the STAT6 signaling pathway was required for the infection-induced attenuation of steatosis but not for improved glucose metabolism, whereas weight loss was attributed to both IL-13/STAT6-dependent and -independent mechanisms. Parasitic nematode infection has both preventive and therapeutic effects against the development of obesity and associated features of metabolic dysfunction in mice. C1 [Yang, Zhonghan; Grinchuk, Viktoriya; Bohl, Jennifer A.; Sun, Rex; Notari, Luigi; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Yang, Zhonghan; Grinchuk, Viktoriya; Bohl, Jennifer A.; Sun, Rex; Notari, Luigi; Shea-Donohue, Terez; Zhao, Aiping] Univ Maryland, Sch Med, Mucosal Biol Res Ctr, Baltimore, MD 21201 USA. [Smith, Allen; Qin, Bolin; Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Zhang, Zhongyan; Sesaki, Hiromi] Johns Hopkins Univ, Sch Med, Dept Cell Biol, Baltimore, MD USA. [Yang, Zhonghan] Sun Yat Sen Univ, Dept Biochem, Zhongshan Med Sch, Guangzhou 510275, Guangdong, Peoples R China. RP Zhao, AP (reprint author), Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. EM azhao@mbrc.umaryland.edu OI Urban, Joseph/0000-0002-1590-8869 FU NIH [R01-DK083418, R01-AI/DK49316]; USDA CRIS project [1235-51000-055] FX This work was supported by NIH grants R01-DK083418 (A.Z.) and R01-AI/DK49316 (T.S.-D.) and by USDA CRIS project 1235-51000-055 (J.F.U.). NR 29 TC 18 Z9 18 U1 0 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD JUN PY 2013 VL 81 IS 6 BP 1905 EP 1914 DI 10.1128/IAI.00053-13 PG 10 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 143GN UT WOS:000318855100005 PM 23509143 ER PT J AU Goptar, IA Shagin, DA Shagina, IA Mudrik, ES Smirnova, YA Zhuzhikov, DP Belozersky, MA Dunaevsky, YE Oppert, B Filippova, IY Elpidina, EN AF Goptar, Irina A. Shagin, Dmitry A. Shagina, Irina A. Mudrik, Elena S. Smirnova, Yulia A. Zhuzhikov, Dmitry P. Belozersky, Mikhail A. Dunaevsky, Yakov E. Oppert, Brenda Filippova, Irina Yu. Elpidina, Elena N. TI A digestive prolyl carboxypeptidase in Tenebrio molitor larvae SO INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article DE Digestive peptidase; Insect digestion; Prolyl carboxypeptidase; Stored product pest; Tenebrio molitor ID PROLYLCARBOXYPEPTIDASE ANGIOTENSINASE-C; MICROVILLAR AMINOPEPTIDASE; CLEAVING ENZYMES; STORAGE PROTEINS; MIDGUT CELLS; PEPTIDASES; PURIFICATION; COLEOPTERA; IDENTIFICATION; PREKALLIKREIN AB Prolyl carboxypeptidase (PRCP) is a lysosomal proline specific serine peptidase that also plays a vital role in the regulation of physiological processes in mammals. In this report, we isolate and characterize the first PRCP in an insect. PRCP was purified from the anterior midgut of larvae of a stored product pest, Tenebrio molitor, using a three-step chromatography strategy, and it was determined that the purified enzyme was a dimer. The cDNA of PRCP was cloned and sequenced, and the predicted protein was identical to the proteomic sequences of the purified enzyme. The substrate specificity and,kinetic parameters of the enzyme were determined. The T. molitor PRCP participates in the hydrolysis of the insect's major dietary proteins, gliadins, and is the first PRCP to be ascribed a digestive function. Our collective data suggest that the evolutionary enrichment of the digestive peptidase complex in insects with an area of acidic to neutral pH in the midgut is a result of the incorporation of lysosomal peptidases, including PRCP. Published by Elsevier Ltd. C1 [Goptar, Irina A.; Filippova, Irina Yu.] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119991, Russia. [Shagin, Dmitry A.] Shemiakin & Ovchinnikov Inst Bioorgan Chem RAS, Moscow 117997, Russia. [Shagin, Dmitry A.; Shagina, Irina A.; Mudrik, Elena S.] Evrogen JSC, Moscow 117997, Russia. [Smirnova, Yulia A.; Belozersky, Mikhail A.; Dunaevsky, Yakov E.; Elpidina, Elena N.] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119991, Russia. [Zhuzhikov, Dmitry P.] Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia. [Oppert, Brenda] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Oppert, B (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM brenda.oppert@ars.usda.gov RI Belozersky, Mikhail/H-5714-2012; Smirnova, Yulia/C-9903-2012 FU Russian Foundation for Basic Research [11-04-93964-SA_a, 12-04-01562-a, 12-03-01057-a, 12-04-31451-mol-a]; ISTC [3455] FX We thank Steve Garczynski for helpful comments. This work was supported by funds from the Russian Foundation for Basic Research (Grant Nos. 11-04-93964-SA_a, 12-04-01562-a, 12-03-01057-a and 12-04-31451-mol-a) and ISTC (Grant No 3455). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. NR 60 TC 8 Z9 10 U1 1 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0965-1748 J9 INSECT BIOCHEM MOLEC JI Insect Biochem. Mol. Biol. PD JUN PY 2013 VL 43 IS 6 BP 501 EP 509 DI 10.1016/j.ibmb.2013.02.009 PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 152NT UT WOS:000319539100002 PM 23499933 ER PT J AU Bauman, JM Keiffer, CH Hiremath, S McCarthy, BC AF Bauman, Jenise M. Keiffer, Carolyn H. Hiremath, Shiv McCarthy, Brian C. TI Soil preparation methods promoting ectomycorrhizal colonization and American chestnut Castanea dentata establishment in coal mine restoration SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE Appalachian Regional Reforestation Initiative; ecological restoration; ectomycorrhizal fungi; Forestry Reclamation Approach; plantfungal interactions; soil subsurface treatments ID SEEDLING ESTABLISHMENT; FUNGAL COMMUNITIES; PRIMARY SUCCESSION; FOREST; TREE; LAND; MYCORRHIZAE; SPECIFICITY; RECLAMATION; COMPETITION AB The objective of this research was to evaluate soil subsurface methods that may aid in seedling establishment and encourage root colonization from a diverse group of ectomycorrhizal (ECM) fungi during restoration projects. American chestnut Castanea dentata Marsh. Borkh. and backcrossed chestnuts seedlings were planted on a reclaimed coal mine site in central Ohio, USA. Roots from chestnut seedlings planted in the plots that were cross-ripped, plowed and disked, or a combination of treatments were sampled for ECM fungi and compared with control plots. The presence and identification of native ECM were determined by fungal DNA sequencing of the internal-transcribed (ITS) region. After two growing seasons, mechanical soil treatments resulted in seedlings with significantly more ECM species when compared to seedlings grown in the control plots (P<0 center dot 0001). A nonmetric multidimensional scaling ordination followed by a permutational manova confirmed significant dissimilarities in community composition between the control and mechanically treated plots (F=0 center dot 24, P=0 center dot 015). Ectomycorrhizal root colonization was significantly higher on the chestnut seedlings sampled from the mechanically treated plots when compared to the control plots (F=10 center dot 63, P<0 center dot 0001). Differences did not exist among the three mechanical treatments. There was also a significant increase in above-ground seedling growth in the plots that were treated with a surface soil method (F=15 center dot 72, P<0 center dot 0001). It is not clear whether ECM activity was the driver of plant growth; regardless, both are strong indicators of healthy tree establishment. Synthesis and applications. This study illustrates that the use of soil subsurface methods increased ectomycorrhizal (ECM) activity and seedling growth. Employing methods that encourage the root colonization by beneficial ECM and promote healthy seedling establishment may aid the long-term survival of chestnuts in restoration projects. This can be applied to other hardwood seedlings used in reforestation in soils compacted after anthropogenic disturbances. C1 [Bauman, Jenise M.; Keiffer, Carolyn H.] Miami Univ, Dept Bot, Oxford, OH 45056 USA. [Hiremath, Shiv] US Forest Serv, USDA, Delaware, OH 43015 USA. [McCarthy, Brian C.] Ohio Univ, Dept Environm & Plant Biol, Athens, OH 45701 USA. RP Bauman, JM (reprint author), Miami Univ, Dept Bot, Oxford, OH 45056 USA. EM baumanjm@miamioh.edu FU US Department of Interior (Office of Surface Mining); US Forest Service [06-JV-11242300-093]; Miami University's Department of Botany FX This study was supported by National Technology and Transfer funds from the US Department of Interior (Office of Surface Mining), part by a Joint Research Venture grant 06-JV-11242300-093 from the US Forest Service and by the Academic Challenge Grant from Miami University's Department of Botany. We thank Steve Castellano, Dr. Erin Douglas, Ryan Homsher, Keith Gilland, Aaron Kennedy, Kirsten Lehtoma, Corie McCament, Jennifer Seabaugh and Steve West. NR 50 TC 3 Z9 4 U1 4 U2 79 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2013 VL 50 IS 3 BP 721 EP 729 DI 10.1111/1365-2664.12070 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 149VZ UT WOS:000319350800019 ER PT J AU James, JJ Sheley, RL Erickson, T Rollins, KS Taylor, MH Dixon, KW AF James, Jeremy J. Sheley, Roger L. Erickson, Todd Rollins, Kim S. Taylor, Michael H. Dixon, Kingsley W. TI A systems approach to restoring degraded drylands SO JOURNAL OF APPLIED ECOLOGY LA English DT Review DE desert; invasion; restoration; state-and-transition models; thresholds ID LAND-USE CHANGE; TRANSITION MODELS; CLIMATE-CHANGE; ECOLOGICAL RESTORATION; VEGETATION DYNAMICS; POPULATION BIOLOGY; SHRUB-STEPPE; STATE; ECOSYSTEM; CONSERVATION AB Drylands support over 2 billion people and are major providers of critical ecosystem goods and services across the globe. Drylands, however, are one of the most susceptible biomes to degradation. International programmes widely recognize dryland restoration as key to combating global dryland degradation and ensuring future global sustainability. While the need to restore drylands is widely recognized and large amounts of resources are allocated to these activities, rates of restoration success remain overwhelmingly low. Advances in understanding the ecology of dryland systems have not yielded proportional advances in our ability to restore these systems. To accelerate progress in dryland restoration, we argue for moving the field of restoration ecology beyond conceptual frameworks of ecosystem dynamics and towards quantitative, predictive systems models that capture the probabilistic nature of ecosystem response to management. To do this, we first provide an overview of conceptual dryland restoration frameworks. We then describe how quantitative systems framework can advance and improve conceptual restoration frameworks, resulting in a greater ability to forecast restoration outcomes and evaluate economic efficiency and decision-making. Lastly, using a case study from the western United States, we show how a systems approach can be integrated with and used to advance current conceptual frameworks of dryland restoration. Synthesis and applications. Systems models for restoration do not replace conceptual models but complement and extend these modelling approaches by enhancing our ability to solve restoration problems and forecast outcomes under changing conditions. Such forecasting of future outcomes is necessary to monetize restoration benefits and cost and to maximize economic benefit of limited restoration dollars. C1 [James, Jeremy J.] Univ Calif, Div Agr & Nat Resources, Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. [Sheley, Roger L.] ARS, USDA, Burns, OR 97720 USA. [Erickson, Todd; Dixon, Kingsley W.] Kings Pk & Bot Gardens, Perth, WA 6005, Australia. [Erickson, Todd; Dixon, Kingsley W.] Univ Western Australia, Fac Nat & Agr Sci, Sch Plant Biol, Crawley, WA 6009, Australia. [Rollins, Kim S.; Taylor, Michael H.] Univ Nevada, Dept Econ, Reno, NV 89557 USA. RP James, JJ (reprint author), Univ Calif, Div Agr & Nat Resources, Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. EM jjjames@ucanr.edu RI Rollins, Kimberly/N-9092-2013; Dixon, Kingsley/A-8133-2016 OI Rollins, Kimberly/0000-0002-0720-006X; Dixon, Kingsley/0000-0001-5989-2929 NR 80 TC 23 Z9 23 U1 6 U2 134 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 J9 J APPL ECOL JI J. Appl. Ecol. PD JUN PY 2013 VL 50 IS 3 BP 730 EP 739 DI 10.1111/1365-2664.12090 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 149VZ UT WOS:000319350800020 ER PT J AU McGarvey, JA Franco, RB Palumbo, JD Hnasko, R Stanker, L Mitloehner, FM AF McGarvey, J. A. Franco, R. B. Palumbo, J. D. Hnasko, R. Stanker, L. Mitloehner, F. M. TI Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE fermentation; lactic acid bacteria; metagenomics ID LACTIC-ACID BACTERIA; GRADIENT GEL-ELECTROPHORESIS; CORN-SILAGE; LACTOBACILLUS-BUCHNERI; AEROBIC DETERIORATION; PROBIOTIC PRODUCTS; HORIZONTAL SILOS; DIVERSITY; PCR; ENSILAGE AB Aims To describe, at high resolution, the bacterial population dynamics and chemical transformations during the ensiling of alfalfa and subsequent exposure to air. Methods and Results Samples of alfalfa, ensiled alfalfa and silage exposed to air were collected and their bacterial population structures compared using 16S rRNA gene libraries containing approximately 1900 sequences each. Cultural and chemical analyses were also performed to complement the 16S gene sequence data. Sequence analysis revealed significant differences (P<0 center dot 05) in the bacterial populations at each time point. The alfalfa-derived library contained mostly sequences associated with the Gammaproteobacteria (including the genera: Enterobacter, Erwinia and Pantoea); the ensiled material contained mostly sequences associated with the lactic acid bacteria (LAB) (including the genera: Lactobacillus, Pediococcus and Lactococcus). Exposure to air resulted in even greater percentages of LAB, especially among the genus Lactobacillus, and a significant drop in bacterial diversity. Conclusions In-depth 16S rRNA gene sequence analysis revealed significant bacterial population structure changes during ensiling and again during exposure to air. Significance and Impact of the Study This in-depth description of the bacterial population dynamics that occurred during ensiling and simulated feed out expands our knowledge of these processes. C1 [McGarvey, J. A.; Palumbo, J. D.; Hnasko, R.; Stanker, L.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Franco, R. B.; Mitloehner, F. M.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. RP McGarvey, JA (reprint author), ARS, USDA, Plant Mycotoxin Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM jeffery.mcgarvey@ars.usda.gov FU California Air Resources Board (CARB) FX The authors would like to thank Mr. Jason Kish for assistance with library construction and sequencing and Ms. Teresa O'Keeffe for quantification of fungi and yeasts. This work was partially funded by a grant from the California Air Resources Board (CARB). NR 54 TC 5 Z9 5 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUN PY 2013 VL 114 IS 6 BP 1661 EP 1670 DI 10.1111/jam.12179 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 146ER UT WOS:000319072900011 PM 23521112 ER PT J AU Wu, M Shen, Q Yang, Y Zhang, S Qu, W Chen, J Sun, HY Chen, SQ AF Wu, Min Shen, Qi Yang, Yong Zhang, Sheng Qu, Wen Chen, Jing Sun, Hongying Chen, Shuqing TI Disruption of YPS1 and PEP4 genes reduces proteolytic degradation of secreted HSA/PTH in Pichia pastoris GS115 SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Heterologous protein expression; Proteolytic degradation; Pichia pastoris; Yapsin; Proteinase A ID PARATHYROID-HORMONE; SACCHAROMYCES-CEREVISIAE; METHYLOTROPHIC YEAST; PROTEASE; EXPRESSION; OSTEOPOROSIS; PROTEINS; REQUIRES; ALBUMIN; ABSENCE AB Human serum albumin (HSA) and human parathyroid hormone (1-34) [PTH (1-34)] fusion protein [HSA/PTH (1-34)] is a promising long-acting form of PTH (1-34) for osteoporosis treatment. Secretory expression of intact HSA/PTH (1-34) in Pichia pastoris GS115 was accompanied by two degradation fragments, with molecular weights around 66 kDa, in addition to the well-known similar to 45 kDa HSA-truncated fragment, resulting in a low yield of intact protein. In this study, two internal cleavage sites were identified in the PTH (1-34) portion of the fusion protein by Western Blot analysis. To minimize proteolytic cleavages, several protease genes including PEP4 (encoding proteinase A), PRB1 (proteinase B) and seven YPSs genes (yapsin family members) were knocked out respectively by disruption of the individual genes and the selective combinations. Reduced degradation was observed by single disruption of either PEP4 gene or YPS1 gene, and the lowest level of degradation was observed in a pep4a-(3)yps1a-(3) double disruptant. After 72 h of induction, more than 80 % of the HSA/PTH (1-34) secreted by the pep4a-(3)yps1a-(3) double disruptant remained intact, in comparison to only 30 % with the wild-type strain. C1 [Wu, Min; Shen, Qi; Qu, Wen; Chen, Jing; Sun, Hongying; Chen, Shuqing] Zhejiang Univ, Coll Pharmaceut Sci, Inst Pharmacol & Toxicol & Biochem Pharmaceut, Hangzhou 310058, Zhejiang, Peoples R China. [Yang, Yong] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Zhang, Sheng] Cornell Univ, Inst Biotechnol & Life Sci Biotechnol, Ithaca, NY 14853 USA. [Chen, Jing] Univ Shizuoka, Sch Pharmaceut Sci, Shizuoka 4228526, Japan. RP Chen, SQ (reprint author), Zhejiang Univ, Coll Pharmaceut Sci, Inst Pharmacol & Toxicol & Biochem Pharmaceut, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. EM chenshuqing@zju.edu.cn OI Chen, Shuqing/0000-0002-0792-3735 FU Science and Technology of Department of Zhejiang Province, China [2007C03001-2, 2010C13006] FX This work was financially supported by two grants (No. 2007C03001-2 and No. 2010C13006) from Science and Technology of Department of Zhejiang Province, China. The authors would like to thank Professor David Wilson for his critical comments of this manuscript and helpful discussion. NR 27 TC 8 Z9 11 U1 0 U2 28 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD JUN PY 2013 VL 40 IS 6 BP 589 EP 599 DI 10.1007/s10295-013-1264-8 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 147KU UT WOS:000319166500007 PM 23529666 ER PT J AU Morris, EE Kepler, RM Long, SJ Williams, DW Hajek, AE AF Morris, E. Erin Kepler, Ryan M. Long, Stefan J. Williams, David W. Hajek, Ann E. TI Phylogenetic analysis of Deladenus nematodes parasitizing northeastern North American Sirex species SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Host specificity; Biological control; Amylostereum; Woodwasp; Invasive species; Forest entomology ID SIRICID WOODWASPS; SOUTHERN-HEMISPHERE; NEOTYLENCHIDAE; NOCTILIO; TYLENCHIDA; SEQUENCES; INFERENCE; MRBAYES; MAFFT AB The parasitic nematode Deladenus siricidicola is a biological control agent of the invasive woodwasp, Sirex noctilio. Since the discovery of S. noctilio in pine forests of northeastern North America in 2005, a biological control program involving the Kamona strain of D. siricidicola has been under consideration. However, North American pine forests have indigenous Sirex spp. and likely harbor a unique assemblage of associated nematodes. We assessed phylogenetic relationships among native Deladenus spp. in the northeastern United States and the Kamona strain of D. siricidicola. We sequenced three genes (mtCO1, LSU, and ITS) from nematodes extracted from parasitized Sirex spp. collected inside and outside of the range of S. noctilio. Our analyses suggest cospeciation between four North American Sirex spp. and their associated nematode parasites. Within two S. noctilio individuals we found nematodes that we hypothesize are normally associated with Sirex nigricornis. One individual of the native S. nigricornis contained Deladenus normally associated with S. noctilio. We discuss nematode-host fidelity in this system and the potential for non-target impacts of a biological control program using D. siricidicola against S. noctilio. (C) 2013 Elsevier Inc. All rights reserved. C1 [Morris, E. Erin; Kepler, Ryan M.; Long, Stefan J.; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Williams, David W.] APHIS, USDA, CPHST, Buzzards Bay, MA 02542 USA. RP Morris, EE (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM eem62@cornell.edu FU USDA APHIS Project [09-8100-1224-CA] FX We greatly appreciate the generous help of Steve Bogdanowicz in providing DNA primers and molecular advice, Dr. Patricia Stock for molecular advice, Dr. Kathie Hodge for careful revisions, Chris Standley for providing S. noctilio samples, Wood Johnson and James Meeker for providing S. nigricornis samples, Gwynne Lim for help with data analysis, and Kenlyn Peters, Isis Caetano, Aaron Anderson, Justin Tyvoll, and Keith Ciccaglione for help with collecting Sirex woodwasps. We are also grateful for funding provided by USDA APHIS Project # 09-8100-1224-CA. NR 36 TC 14 Z9 15 U1 1 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUN PY 2013 VL 113 IS 2 BP 177 EP 183 DI 10.1016/j.jip.2013.03.003 PG 7 WC Zoology SC Zoology GA 145QB UT WOS:000319030800008 PM 23542205 ER PT J AU Glenn, LM Lindsey, RL Folster, JP Pecic, G Boerlin, P Gilmour, MW Harbottle, H Zhao, SH McDermott, PF Fedorka-Cray, PJ Frye, JG AF Glenn, LaShanda M. Lindsey, Rebecca L. Folster, Jason P. Pecic, Gary Boerlin, Patrick Gilmour, Mathew W. Harbottle, Heather Zhao, Shaohua McDermott, Patrick F. Fedorka-Cray, Paula J. Frye, Jonathan G. TI Antimicrobial Resistance Genes in Multidrug-Resistant Salmonella enterica Isolated from Animals, Retail Meats, and Humans in the United States and Canada SO MICROBIAL DRUG RESISTANCE LA English DT Article ID PATHOGENIC ESCHERICHIA-COLI; SEROVAR TYPHIMURIUM; FOOD ANIMALS; PLASMIDS; MICROARRAY; STRAINS; SEQUENCE; IDENTIFICATION; POPULATIONS; HEIDELBERG AB Salmonella enterica is a prevalent foodborne pathogen that can carry multidrug resistance (MDR) and pose a threat to human health. Identifying the genetics associated with MDR in Salmonella isolated from animals, foods, and humans can help determine sources of MDR in food animals and their impact on humans. S. enterica serovars most frequently carrying MDR from healthy animals, retail meats, and human infections in the United States and Canada were identified and isolates resistant to the largest number of antimicrobials were chosen. Isolates were from U. S. slaughter (n = 12), retail (9), and humans (9), and Canadian slaughter (9), retail (9), and humans (8; total n = 56). These isolates were assayed by microarray for antimicrobial resistance and MDR plasmid genes. Genes detected encoded resistance to aminoglycosides (alleles of aac, aad, aph, strA/B); betalactams (blaTEM, blaCMY, blaPSE-1); chloramphenicol (cat, flo, cmlA); sulfamethoxazole (sulI); tetracycline (tet(A, B, C, D) and tetR); and trimethoprim (dfrA). Hybridization with IncA/C plasmid gene probes indicated that 27/56 isolates carried one of these plasmids; however, they differed in several variable regions. Cluster analysis based on genes detected separated most of the isolates into two groups, one with IncA/C plasmids and one without IncA/C plasmids. Other plasmid replicons were detected in all but one isolate, and included I1 (25/56), N (23/56), and FIB (10/56). The presence of different mobile elements along with similar resistance genes suggest that these genetic elements may acquire similar resistance cassettes, and serve as multiple sources for MDR in Salmonella from food animals, retail meats, and human infections. C1 [Glenn, LaShanda M.; Lindsey, Rebecca L.; Fedorka-Cray, Paula J.; Frye, Jonathan G.] USDA, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Res Ctr, ARS, Athens, GA 30605 USA. [Folster, Jason P.; Pecic, Gary] Ctr Dis Control & Prevent, Atlanta, GA USA. [Boerlin, Patrick] Ontario Vet Coll, Guelph, ON, Canada. [Gilmour, Mathew W.] Publ Hlth Agcy Canada, Winnipeg, MB, Canada. [Harbottle, Heather; Zhao, Shaohua; McDermott, Patrick F.] US FDA, Ctr Vet Med, Laurel, MD USA. RP Frye, JG (reprint author), USDA, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Res Ctr, ARS, 950 Coll Stn Rd, Athens, GA 30605 USA. EM jonathan.frye@ars.usda.gov RI Frye, Jonathan/I-6382-2013 OI Frye, Jonathan/0000-0002-8500-3395 FU USDA [6612-32000-006-00] FX The authors thank Jennifer Turpin, Georgina Hidalgo, Jovita Haro, Benny Barrett, and Takiyah Ball at USDA for technical assistance. From CDC we thank Jean Whichard for helpful conversations and editing the manuscript. From CIPARS we thank Lucie Dutil for isolate selection, and Laura Martin and Emily Weir for DNA isolation. We would also like to dedicate this article in memory of our colleague, Lucie Dutil, whom we had the privilege to know and work with through NARMS and CIPARS collaborations. This work was supported by USDA project number 6612-32000-006-00. NR 43 TC 14 Z9 14 U1 3 U2 25 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD JUN PY 2013 VL 19 IS 3 BP 175 EP 184 DI 10.1089/mdr.2012.0177 PG 10 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA 150OG UT WOS:000319399900004 PM 23350745 ER PT J AU Sjolund-Karlsson, M Howie, RL Blickenstaff, K Boerlin, P Ball, T Chalmers, G Duval, B Haro, J Rickert, R Zhao, SH Fedorka-Cray, PJ Whichard, JM AF Sjolund-Karlsson, Maria Howie, Rebecca L. Blickenstaff, Karen Boerlin, Patrick Ball, Takiyah Chalmers, Gabhan Duval, Brea Haro, Jovita Rickert, Regan Zhao, Shaohua Fedorka-Cray, Paula J. Whichard, Jean M. TI Occurrence of beta-Lactamase Genes Among Non-Typhi Salmonella enterica Isolated from Humans, Food Animals, and Retail Meats in the United States and Canada SO MICROBIAL DRUG RESISTANCE LA English DT Article ID CEFTRIAXONE-RESISTANT SALMONELLA; EXTENDED-SPECTRUM CEPHALOSPORINS; MULTIDRUG-RESISTANT; ESCHERICHIA-COLI; ANTIMICROBIAL RESISTANCE; SEROTYPE; CMY-2; PREVALENCE; EMERGENCE; INFECTION AB Non-Typhi Salmonella cause over 1.7 million cases of gastroenteritis in North America each year, and food-animal products are commonly implicated in human infections. For invasive infections, antimicrobial therapy is indicated. In North America, the antimicrobial susceptibility of Salmonella is monitored by the U. S. National Antimicrobial Resistance Monitoring System (NARMS) and The Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). In this study, we determined the susceptibility to cephalosporins by broth microdilution among 5,041 non-Typhi Salmonella enterica isolated from food animals, retail meats, and humans. In the United States, 109 (4.6%) of isolates collected from humans, 77 (15.7%) from retail meat, and 140 (10.6%) from food animals displayed decreased susceptibility to cephalosporins (DSC). Among the Canadian retail meat and food animal isolates, 52 (13.0%) and 42 (9.4%) displayed DSC. All isolates displaying DSC were screened for beta-lactamase genes (bla(TEM), bla(SHV), bla(CMY), bla(CTX-M), and bla(OXA-1)) by polymerase chain reaction. At least one beta-lactamase gene was detected in 74/109 (67.9%) isolates collected from humans, and the blaCMY genes were most prevalent (69/109; 63.3%). Similarly, the blaCMY genes predominated among the beta-lactamase-producing isolates collected from retail meats and food animals. Three isolates from humans harbored a bla(CTX-M-15) gene. No animal or retail meat isolates harbored a bla(CTX-M) or bla(OXA-1) gene. A bla(TEM) gene was found in 5 human, 9 retail meat, and 17 animal isolates. Although serotype distributions varied among human, retail meat, and animal sources, overlap in bla(CMY)-positive serotypes across sample sources supports meat and food-animal sources as reservoirs for human infection. C1 [Sjolund-Karlsson, Maria; Rickert, Regan; Whichard, Jean M.] Ctr Dis Control & Prevent, Natl Antimicrobial Resistance Monitoring Syst, Atlanta, GA 30329 USA. [Howie, Rebecca L.] IHRC Inc, Atlanta, GA USA. [Blickenstaff, Karen; Zhao, Shaohua] US FDA, Laurel, MD USA. [Boerlin, Patrick; Chalmers, Gabhan] Ontario Vet Coll, Guelph, ON, Canada. [Ball, Takiyah; Duval, Brea; Haro, Jovita; Fedorka-Cray, Paula J.] USDA, Athens, GA USA. RP Sjolund-Karlsson, M (reprint author), Ctr Dis Control & Prevent, Natl Antimicrobial Resistance Monitoring Syst, OID NCEZID DFWED EDLB, 1600 Clifton Rd,Mail Stop G29, Atlanta, GA 30329 USA. EM fwt4@cdc.gov FU CDC; FDA-CVM; Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada FX We thank the NARMS-participating public health laboratories, the Retail Foods Survey Working Group, and the FSIS laboratories for submitting the isolates. We also thank the CO, MO, and NY Divisions of Public Health for providing patient interviews. This work was supported by an interagency agreement between CDC and FDA-CVM. Regarding CIPARS abattoir isolates, we thank the abattoir industry personnel and the Canadian Food Inspection Agency regional directors, inspection managers, and onsite staff for their extensive voluntary participation. The CIPARS farm surveillance component thanks all participating veterinarians and producers, the CIPARS Farm Swine Surveillance Advisory Committee as well as Agriculture and Agri-Food Canada, the provincial ministries of agriculture in BC, SK, AB, and QC, and the USDA, CAHFSE Program. The CIPARS retail component thanks the field technicians as well as the University of Prince Edward Island. Lastly, CIPARS thanks all laboratory technicians and data management staff for their contributions to the overall program. The molecular characterization of Canadian isolates was financially supported by the Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario, Canada. NR 52 TC 8 Z9 8 U1 0 U2 11 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1076-6294 J9 MICROB DRUG RESIST JI Microb. Drug Resist. PD JUN PY 2013 VL 19 IS 3 BP 191 EP 197 DI 10.1089/mdr.2012.0178 PG 7 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA 150OG UT WOS:000319399900006 PM 23289438 ER PT J AU Lin, H Gudmestad, NC AF Lin, Hong Gudmestad, Neil C. TI Aspects of Pathogen Genomics, Diversity, Epidemiology, Vector Dynamics, and Disease Management for a Newly Emerged Disease of Potato: Zebra Chip SO PHYTOPATHOLOGY LA English DT Review DE genetic diversity; potato psyllid; psyllid Bactericera cockerelli ID CANDIDATUS LIBERIBACTER SOLANACEARUM; PSYLLID BACTERICERA-COCKERELLI; REAL-TIME PCR; 1ST REPORT; HEMIPTERA TRIOZIDAE; NEW-ZEALAND; UNITED-STATES; SOLANUM-TUBEROSUM; AFFECTED CARROTS; SALICYLIC-ACID AB An overview is provided for the aspects of history, biology, genomics, genetics, and epidemiology of zebra chip (ZC), a destructive disease of potato (Solanum tuberosum) that represents a major threat to the potato industries in the United States as well as other potato-production regions in the world. The disease is associated with a gram-negative, phloem-limited, insect-vectored, unculturable prokaryote, Candidatus Liberibacter solanacearum', that belongs to the Rhizobiaceae family of alpha-Proteobacteria. The closest cultivated relatives of 'Ca. L. solanacearum' are members of the group of bacteria known as the alpha-2 subgroup. In spite of the fact that Koch's postulates sensu stricto have not been fulfilled, a great deal of progress has been made in understanding the ZC disease complex since discovery of the disease. Nevertheless, more research is needed to better understand vector biology, disease mechanisms, host response, and epidemiology in the context of vector pathogen plant interactions. Current ZC management strategies focus primarily on psyllid control. The ultimate control of ZC likely relies on host resistance. Unfortunately, all commercial potato cultivars are susceptible to ZC. Elucidation of the 'Ca. L. solanacearum' genome sequence has provided insights into the genetic basis of virulence and physiological and metabolic capability of this organism Finally, the most effective, sustainable management of ZC is likely to be based on integrated strategies, including removal or reduction of vectors or inocula, improvement of host resistance to the presumptive pathogen and psyllid vectors, and novel gene-based therapeutic treatment. C1 [Lin, Hong] ARS, USDA, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. [Gudmestad, Neil C.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Lin, H (reprint author), ARS, USDA, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM hong.lin@ars.usda.gov FU United States Department of Agriculture NIFA-SCRI [2009-51181-20176] FX Trade names or commercial products in this publication are mentioned solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer.This work was supported by a grant from the United States Department of Agriculture NIFA-SCRI (Project number 2009-51181-20176). We thank E. Civerolo for critical reading of this review article. NR 115 TC 10 Z9 11 U1 4 U2 44 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 BP 524 EP 537 DI 10.1094/PHYTO-09-12-0238-RVW PG 14 WC Plant Sciences SC Plant Sciences GA 150EE UT WOS:000319372200001 PM 23268582 ER PT J AU Hilf, ME Sims, KR Folimonova, SY Achor, DS AF Hilf, Mark E. Sims, Kenneth R. Folimonova, Svetlana Y. Achor, Diann S. TI Visualization of 'Candidatus Liberibacter asiaticus' Cells in the Vascular Bundle of Citrus Seed Coats with Fluorescence In Situ Hybridization and Transmission Electron Microscopy SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; CA. L. AMERICANUS; 16S RIBOSOMAL-RNA; REAL-TIME PCR; GREENING DISEASE; HUANGLONGBING DISEASE; XYLELLA-FASTIDIOSA; SWEET ORANGE; DIVERSITY; BACTERIUM AB 'Candidatus Liberibacter asiaticus' is the bacterium implicated as a causal agent of the economically damaging disease of citrus called huanglongbing (HLB). Vertical transmission of the organism through seed to the seedling has not been demonstrated. Previous studies using real-time polymerase chain reaction assays indicated abundant bacterial 16S rRNA sequences in seed coats of citrus seed but the presence of intact bacterial cells was not demonstrated. We used microscopy to verify that intact bacterial cells were present in citrus seed coats. Bacterial cells with the morphology and physical dimensions appropriate for 'Ca. L. asiaticus' were seen in phloem sieve elements in the vascular bundle of grapefruit seed coats using transmission electron microscopy (TEM). Fluorescence in situ hybridization (FISH) analyses utilizing probes complementary to the 'Ca. L. asiaticus' 16S rRNA gene revealed bacterial cells in the vascular tissue of intact seed coats of grapefruit and pummelo and in fragmented vascular bundles excised from grapefruit seed coats. The physical measurements and the morphology of individual bacterial cells were consistent with those ascribed in the literature to 'Ca. L. asiaticus'. No bacterial cells were observed in preparations of seed from fruit from noninfected trees. A small library of clones amplified from seed coats from a noninfected tree using degenerate primers targeting prokaryote 16S rRNA gene sequences contained no 'Ca. L. asiaticus' sequences, whereas 95% of the sequences in a similar library from DNA from seed coats from an infected tree were identified as 'Ca. L. asiaticus', providing molecular genetic corroboration that the bacterial cells observed by TEM and FISH in seed coats from infected trees were 'Ca. L. asiaticus'. C1 [Hilf, Mark E.; Sims, Kenneth R.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Folimonova, Svetlana Y.; Achor, Diann S.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33805 USA. RP Hilf, ME (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM mark.hilf@ars.usda.gov NR 40 TC 7 Z9 8 U1 4 U2 43 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 BP 545 EP 554 DI 10.1094/PHYTO-09-12-0226-R PG 10 WC Plant Sciences SC Plant Sciences GA 150EE UT WOS:000319372200003 PM 23676087 ER PT J AU Park, JH Juzwik, J Cavender-Bares, J AF Park, J. -H. Juzwik, J. Cavender-Bares, J. TI Multiple Ceratocystis smalleyi Infections Associated with Reduced Stem Water Transport in Bitternut Hickory SO PHYTOPATHOLOGY LA English DT Article ID HEAT PULSE VELOCITY; SAP FLOW; RAFFAELEA-QUERCIVORA; MOUNTAIN PINE; WILT DISEASE; SAPWOOD; BEETLE; INOCULATION; CAVITATION; FAGACEAE AB Hundreds of cankers caused by Ceratocystis smalleyi are associated with hickory bark beetle-attacked bittenut hickory exhibiting rapid crown decline in the north-central and northeastern United States. Discolored sapwood colonized by the fungus commonly underlies the cankers. Field studies were conducted to test the hypothesis that C. smalleyi infections cause vascular system dysfunction in infected trees. Fifty C. smalleyi inoculations made at 1.8 to 3.8 m in height on stems of healthy bitternut hickory trees (13 to 28 cm in diameter at 1.4 m in height) resulted in extensive canker formation and sapwood discoloration 12 to 14 months after treatment compared with water-inoculated and noninoculated controls. Sap flow velocity (midday) was significantly lower in the infected trees compared with that in the controls. Sap flow velocity also was inversely correlated with the proportion of bark area with cankered tissues and with tylose abundance in the youngest two growth rings. Tylose formation in current-year vessels associated with C. smalleyi infections is likely responsible for much of the water transport disruption. It is hypothesized that multiple stem infections of C. smalleyi and the resulting xylem dysfunction contribute to crown wilt development in bitternut hickory exhibiting rapid crown decline. C1 [Park, J. -H.] Korea Forest Res Inst, Seoul, South Korea. [Juzwik, J.] USDA, St Paul, MN 55108 USA. [Cavender-Bares, J.] Univ Minnesota, St Paul, MN 55108 USA. RP Juzwik, J (reprint author), USDA, St Paul, MN 55108 USA. EM jjuzwik@fs.fed.us RI Cavender-Bares, Jeannine/K-5716-2013 FU United States Department of Agriculture Forest Service, Forest Health Evaluation Monitoring Program [NC-EM-07-01]; Department of Plant Pathology, University of Minnesota FX Mention of trade names does not constitute endorsement by the United States Department of Agriculture. Field sites and trees used for this research were provided by management foresters of the Carly State Park, Minnesota Department of Natural Resources and the Chippewa County Forest in Wisconsin. The research was supported, in part, by the United States Department of Agriculture Forest Service, Forest Health Evaluation Monitoring Program (project number NC-EM-07-01). Partial funding also was provided by the Department of Plant Pathology, University of Minnesota. We thank A. Klein, B. Stroobants, B. Zenner, C. Shaw, K. Koehler, K. Sweeney, M. Boll, and P. Castillo for their technical assistance; K. T. Smith, R. Seavey, A. Wiedenhoeft, E. Peters, and J. Shannon for providing equipment, supplies, and instructions; M. Ostry, J. Van Sambeek, K. T. Smith, and J. Stanovick for presubmission review of the manuscript and many helpful suggestions; and two anonymous reviewers for additional suggestions. NR 41 TC 9 Z9 9 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 BP 565 EP 574 DI 10.1094/PHYTO-10-12-0272-R PG 10 WC Plant Sciences SC Plant Sciences GA 150EE UT WOS:000319372200005 PM 23360533 ER PT J AU Mammella, MA Martin, FN Cacciola, SO Coffey, MD Faedda, R Schena, L AF Mammella, Marco A. Martin, Frank N. Cacciola, Santa O. Coffey, Michael D. Faedda, Roberto Schena, Leonardo TI Analyses of the Population Structure in a Global Collection of Phytophthora nicotianae Isolates Inferred from Mitochondrial and Nuclear DNA Sequences SO PHYTOPATHOLOGY LA English DT Article DE intraspecific variability ID GENETIC DIVERSITY; MEFENOXAM SENSITIVITY; ORNAMENTAL PLANTS; INFESTANS; PARASITICA; NURSERIES; MARKERS; TOBACCO; GENOME; ORIGIN AB Genetic variation within the heterothallic cosmopolitan plant pathogen Phytophthora nicotianae was determined in 96 isolates from a wide range of hosts and geographic locations by characterizing four mitochondrial (10% of the genome) and three nuclear loci. In all, 52 single-nucleotide polymorphisms (SNPs) (an average of 1 every 58 bp) and 313 sites with gaps representing 5,450 bases enabled the identification of 50 different multilocus mitochondrial haplotypes. Similarly, 24 SNPs (an average of 1 every 69 bp), with heterozygosity observed at each locus, were observed in three nuclear regions (hyp, scp, and beta-tub) differentiating 40 multilocus nuclear genotypes. Both mitochondrial and nuclear markers revealed a high level of dispersal of isolates and an inconsistent geographic structuring of populations. However, a specific association was observed for host of origin and genetic grouping with both nuclear and mitochondrial sequences. In particular, the majority of citrus isolates from Italy, California, Florida, Syria, Albania, and the Philippines clustered in the same mitochondrial group and shared at least one nuclear allele. A similar association was also observed for isolates recovered from Nicotiana and Solanum spp. The present study suggests an important role of nursery populations in increasing genetic recombination within the species and the existence of extensive phenomena of migration of isolates that have been likely spread worldwide with infected plant material. C1 [Mammella, Marco A.; Schena, Leonardo] Univ Mediterranea, Dipartimento Agr, I-89122 Reggio Di Calabria, Italy. [Martin, Frank N.] ARS, USDA, Salinas, CA 93905 USA. [Cacciola, Santa O.; Faedda, Roberto] Univ Catania, Dipartimento Gest Sistemi Agroalimentari & Ambien, I-95123 Catania, Italy. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Schena, L (reprint author), Univ Mediterranea, Dipartimento Agr, I-89122 Reggio Di Calabria, Italy. EM Ischena@unirc.it OI Schena, Leonardo/0000-0002-9737-2593; Cacciola, Santa Olga/0000-0001-7926-3601 FU MIUR-FIRB; United States Department of Agriculture Agriculture and Food Research Initiative Plan Biosecurity Competitive Grant [2007-55605-17835, 2008-55605-18773] FX This work was partially funded by MIUR-FIRB 2010 "Metagenomic strategies to assess genetic diversity in soil-borne Phytophthora species." Much of this research was completed by M. A. Mammella while working in the laboratory of F. N. Martin and partially supported by United States Department of Agriculture Agriculture and Food Research Initiative Plan Biosecurity Competitive Grant 2007-55605-17835 and 2008-55605-18773. We thank F. Mercati (Dipartimento di Agraria, Universita degli Studi Mediterranea, Reggio Calabria, Italy) for his help on AMOVA analyses; and A. Pane (Dipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Universita di Catania, Italy), A. Ippolito (Dipartimento di Biologia e Chimica Agro-forestale e Ambientale, Universita di Bari, Italy), and D. E. L. Cooke (James Hutton Institute, Dundee, Scotland, UK) for providing isolates. NR 65 TC 11 Z9 12 U1 1 U2 26 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 BP 610 EP 622 DI 10.1094/PHYTO-10-12-0263-R PG 13 WC Plant Sciences SC Plant Sciences GA 150EE UT WOS:000319372200010 PM 23384862 ER PT J AU Robert, CAM Erb, M Hiltpold, I Hibbard, BE Gaillard, MDP Bilat, J Degenhardt, J Cambet-Petit-Jean, X Turlings, TCJ Zwahlen, C AF Robert, Christelle Aurelie Maud Erb, Matthias Hiltpold, Ivan Hibbard, Bruce Elliott Gaillard, Mickael David Philippe Bilat, Julia Degenhardt, Joerg Cambet-Petit-Jean, Xavier Turlings, Ted Christiaan Joannes Zwahlen, Claudia TI Genetically engineered maize plants reveal distinct costs and benefits of constitutive volatile emissions in the field SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE terpenoid-engineered plants; volatile emission costs; volatile emission benefits; (E)-beta-caryophyllene; belowground herbivory; aboveground herbivory ID INDUCED RESISTANCE; TRADE-OFFS; SPODOPTERA-LITTORALIS; TERPENOID METABOLISM; NICOTIANA-ATTENUATA; LARVAL DEVELOPMENT; INSECT HERBIVORES; INDIRECT DEFENSES; ECOLOGICAL COSTS; BACKGROUND ODOR AB Genetic manipulation of plant volatile emissions is a promising tool to enhance plant defences against herbivores. However, the potential costs associated with the manipulation of specific volatile synthase genes are unknown. Therefore, we investigated the physiological and ecological effects of transforming a maize line with a terpene synthase gene in field and laboratory assays, both above- and below ground. The transformation, which resulted in the constitutive emission of (E)--caryophyllene and -humulene, was found to compromise seed germination, plant growth and yield. These physiological costs provide a possible explanation for the inducibility of an (E)--caryophyllene-synthase gene in wild and cultivated maize. The overexpression of the terpene synthase gene did not impair plant resistance nor volatile emission. However, constitutive terpenoid emission increased plant apparency to herbivores, including adults and larvae of the above ground pest Spodoptera frugiperda, resulting in an increase in leaf damage. Although terpenoid overproducing lines were also attractive to the specialist root herbivore Diabrotica virgifera virgifera below ground, they did not suffer more root damage in the field, possibly because of the enhanced attraction of entomopathogenic nematodes. Furthermore, fewer adults of the root herbivore Diabrotica undecimpunctata howardii were found to emerge near plants that emitted (E)--caryophyllene and -humulene. Yet, overall, under the given field conditions, the costs of constitutive volatile production overshadowed its benefits. This study highlights the need for a thorough assessment of the physiological and ecological consequences of genetically engineering plant signals in the field to determine the potential of this approach for sustainable pest management strategies. C1 [Robert, Christelle Aurelie Maud; Erb, Matthias; Hiltpold, Ivan; Gaillard, Mickael David Philippe; Bilat, Julia; Cambet-Petit-Jean, Xavier; Turlings, Ted Christiaan Joannes; Zwahlen, Claudia] Univ Neuchatel, Lab Fundamental & Appl Res Chem Ecol FARCE, CH-2000 Neuchatel, Switzerland. [Robert, Christelle Aurelie Maud; Erb, Matthias] Max Planck Inst Chem Ecol, Root Herbivore Interact Grp, Jena, Germany. [Robert, Christelle Aurelie Maud] Max Planck Inst Chem Ecol, Dept Biochem, Jena, Germany. [Hibbard, Bruce Elliott] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Degenhardt, Joerg] Univ Halle Wittenberg, Inst Pharm, D-06108 Halle, Germany. RP Turlings, TCJ (reprint author), Univ Neuchatel, Lab Fundamental & Appl Res Chem Ecol FARCE, CH-2000 Neuchatel, Switzerland. EM ted.turlings@unine.ch RI Turlings, Ted/E-8671-2012; Hiltpold, Ivan/D-1666-2016; OI Turlings, Ted/0000-0002-8315-785X; Hiltpold, Ivan/0000-0001-6374-6684; Erb, Matthias/0000-0002-4446-9834 FU Swiss National Science Foundation [FN 31000AO-107974, FN 405940-115715]; National Centre of Competence in Research NCCR FX We thank Matt Higdon, Rebecca Bukowski, Sarah Zukoff, Julie Barry and the whole student crew for their kind contribution to field experiments. Wade French and Chad Nielson (USDA-ARS-NACRL Brookings, USA) supplied D. virgifera eggs. Andrew Brown from Becker Underwood Ltd (Littlehampton, UK) provided entomopathogenic nematodes for field experiments. Ian Kaplan and two anonymous reviewers provided helpful comments on an earlier version of this manuscript. Research activities by C.A.M.R., M.E., I.H., J.B., X.C.P.J., T.C.J.T. and C.Z. were supported by the Swiss National Science Foundation (FN 31000AO-107974 and FN 405940-115715). This project was partially funded by the National Centre of Competence in Research NCCR. NR 84 TC 39 Z9 40 U1 3 U2 103 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD JUN PY 2013 VL 11 IS 5 BP 628 EP 639 DI 10.1111/pbi.12053 PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA 147FI UT WOS:000319151000011 PM 23425633 ER PT J AU Weiland, JE Beck, BR Davis, A AF Weiland, Jerry E. Beck, Bryan R. Davis, Anne TI Pathogenicity and Virulence of Pythium Species Obtained from Forest Nursery Soils on Douglas-Fir Seedlings SO PLANT DISEASE LA English DT Article ID IDENTIFICATION; EPIDEMIOLOGY AB Pythium species are common soilborne oomycetes that occur in forest nursery soils throughout the United States. Numerous species have been described from nursery soils. However, with the exception of P. aphanidermatum, P irregulare, P. sylvaticum, and P ultimum, little is known about the potential for other Pythium species found in nursery soils to cause damping-off of tree seedlings. A greenhouse study was conducted to evaluate the pathogenicity and virulence of 44 Pythium isolates representing 16 species that were originally recovered from soil at three forest nurseries in Washington and Oregon. Seeds of Douglas-fir (Pseudotsuga menziesii) were planted into soil infested with each of the isolates. Seedling survival, the number of surviving seedlings with necrotic root lesions, and taproot length were evaluated 4 weeks later. Responses of Douglas-fir to inoculation varied significantly depending on Pythium species and isolate. Eight species (P dissotocum, P. irregulare, P aff. macrosporum, P. mamillatum, P aff oopapillum, P rostratifingens, P. sylvaticum, and P. ultimum var. ultimum) significantly reduced the number of surviving seedlings compared to the noninoculated treatment. However, all Pythium species caused a greater percentage of seedlings to develop root lesions (total mean 40%) than was observed from noninoculated seedlings (17%). Taproot length varied little among Pythium treatments and was not a useful character for evaluating pathogenicity. Results confirm the ability of P. irregulare, P. mamillatum, and P ultimum var. ultimum to cause damping-off of Douglas-fir seedlings, and are indicative that other species such as P. dissotocum, P. aff macrosporum, P. aff oopapillum, P rostratifingens, and P. sylvaticum may also be responsible for seedling loss. C1 [Weiland, Jerry E.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. EM Jerry.Weiland@ars.usda.gov FU Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives FX We gratefully acknowledge the assistance of Val Fieland and the Nik Grunwald lab in the identification of Pythium isolates. We also acknowledge the technical assistance of Duncan Kroese. Financial support was provided by the Pacific Area Wide Pest Management Program for Methyl Bromide Alternatives. NR 19 TC 12 Z9 13 U1 0 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 744 EP 748 DI 10.1094/PDIS-09-12-0895-RE PG 5 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300006 ER PT J AU Yan, GP Smiley, RW Okubara, PA Skantar, AM Reardon, CL AF Yan, Guiping Smiley, Richard W. Okubara, Patricia A. Skantar, Andrea M. Reardon, Catherine L. TI Developing a Real-Time PCR Assay for Detection and Quantification of Pratylenchus neglectus in Soil SO PLANT DISEASE LA English DT Article ID ROOT-LESION NEMATODES; POLYMERASE-CHAIN-REACTION; FRAGMENT-LENGTH-POLYMORPHISM; NORTHWEST UNITED-STATES; POTATO-CYST-NEMATODE; PACIFIC-NORTHWEST; QUANTITATIVE DETECTION; VERTICILLIUM-DAHLIAE; GENUS PRATYLENCHUS; DNA AB Pratylenchus neglectus is one of the most widespread and economically important nematodes that invades plant roots and restricts wheat productivity in the Pacific Northwest. It is challenging to quantify P neglectus using microscopic methods for studies that require large-scale sampling, such as assessment of rotation crops, wheat cultivars, and other management practices. A real-time quantitative polymerase chain reaction (qPCR) assay was developed to detect and quantify P. neglectus from DNA extracts of soil. The primers, designed from the internal transcribed spacer region of rDNA, showed high specificity with a single melt curve peak to DNA from eight isolates of P. neglectus but did not amplify DNA from 28 isolates of other plant-parasitic and non-plant-parasitic nematodes: A standard curve (R-2 = 0.96; P < 0.001) was generated by amplifying DNA extracted from soil to which nematodes were added. The soil standard curve was validated using sterilized soil inoculated with lower numbers of P. neglectus. A significant positive relationship (R-2 = 0.66; P < 0.001) was observed for nematode numbers quantified from 15 field soils using qPCR and the Whitehead tray and microscopic method but the qPCR generally tended to provide higher estimates. Real-time PCR potentially provides a useful platform for efficient detection and quantification of P. neglectus directly from field soils. C1 [Yan, Guiping; Smiley, Richard W.] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. [Okubara, Patricia A.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Skantar, Andrea M.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. [Reardon, Catherine L.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. RP Yan, GP (reprint author), Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. EM guiping.yan@oregonstate.edu FU Idaho Wheat Commission; Oregon Wheat Commission; Washington Wheat Commission; USDA-ARS [5248-22000-012-00D]; Oregon State University [SCA 58-5348-9-100]; United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [SCA 58-5348-9-100] FX This research was funded by the Idaho Wheat Commission, Oregon Wheat Commission, and Washington Wheat Commission; a cooperative agreement between Oregon State University and the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) (SCA 58-5348-9-100, "Control of Root Diseases of Wheat and Barley"); and USDA-ARS Project number 5248-22000-012-00D (P. A. Okubara). We thank S. H. Hulbert and C. Yin at Washington State University for access to the NanoDrop ND-1000 Spectrophotometer, and A. Dyer for providing isolates of control nematode species. NR 44 TC 15 Z9 17 U1 0 U2 30 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 757 EP 764 DI 10.1094/PDIS-08-12-0729-RE PG 8 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300008 ER PT J AU Bassanezi, RB Montesino, LH Gimenes-Fernandes, N Yamamoto, PT Gottwald, TR Amorim, L Bergamin, A AF Bassanezi, Renato B. Montesino, Luiz H. Gimenes-Fernandes, Nelson Yamamoto, Pedro T. Gottwald, Tim R. Amorim, Lilian Bergamin Filho, Armando TI Efficacy of Area-Wide Inoculum Reduction and Vector Control on Temporal Progress of Huanglongbing in Young Sweet Orange Plantings SO PLANT DISEASE LA English DT Article ID CANDIDATUS-LIBERIBACTER-ASIATICUS; SAO-PAULO STATE; GREENING DISEASE; CITRUS; BRAZIL; MANAGEMENT; PCR AB Huanglongbing (HLB), caused by 'Candidatus Liberibacter' spp. and transmitted by the Asian citrus psyllid Diaphorina citri (ACP), is an important threat to citrus industries worldwide, causing significant yield loss. The current recommended strategies to manage HLB are to eliminate HLB-symptomatic trees to reduce sources of bacterial inoculum and to apply insecticides to reduce psyllid vector populations. The objective of this study was to assess the effectiveness and the importance of both strategies applied within young citrus plots (local management), in different frequencies and combinations, on HLB temporal progress. Two factorial field experiments, E1 and E2, were initiated in a new plantation of sweet orange in an HLB epidemic region of Sao Paulo, Brazil, in October 2005 and May 2006, respectively. Local inoculum reduction (tree removal) intervals for E1 were every 4, 8, and 16 weeks and, for E2, every 2, 4, 12, and 26 weeks. Local vector control strategies for E1 were no control, program A (PA), and program B (PB); and, for E2, no control and program C (PC), as follows. Psyllids were controlled with two 56-day-interval soil or drench applications of systemic insecticides concurrently with the rainy season each year and, during the rest of the year, with insecticide sprays every 28 days for PA and every 14 days for PB and PC. Regional HLB management was present for E1 and absent for E2. The beginning of the HLB epidemic was delayed for 10 months in E1 compared with appearance of the first diseased tree in E2 but wasn't affected by different local strategies in either experiment. After 60 (E1) and 53 (E2) months, the HLB incidence and progress rates were not affected by different frequencies of local inoculum reduction in either experiment, and were different only in plots with and without local vector control in E2. In E1, the disease incidence was reduced by 90% and the disease progress rate by 50% in plots both with and without vector control. These reductions were explained by smaller psyllid populations and lower frequency of bacterialiferous psyllids in E1 compared with E2. Annual productivity increased over time in E1, as expected for young plantings, but remained stable or decreased in E2. These results confirm that immigration of bacterialiferous ACP vectors plays a critical role in HLB epidemics and suggest that area-wide inoculum reduction and ACP management strongly affect HLB control. C1 [Bassanezi, Renato B.; Montesino, Luiz H.; Gimenes-Fernandes, Nelson] Fundo Def Citricultura, Dept Cient, BR-14807040 Araraquara, SP, Brazil. [Yamamoto, Pedro T.] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP, Brazil. [Gottwald, Tim R.] USDA ARS, USHRL, Ft Pierce, FL 34945 USA. [Amorim, Lilian; Bergamin Filho, Armando] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP, Brazil. RP Bassanezi, RB (reprint author), Fundo Def Citricultura, Dept Cient, BR-14807040 Araraquara, SP, Brazil. EM rbbassanezi@fundecitrus.com.br RI Amorim, Lilian /C-2782-2012; Montesino, Luiz Henrique/D-5491-2014; Yamamoto, Pedro/C-5069-2012 OI Amorim, Lilian /0000-0001-9793-0850; Yamamoto, Pedro/0000-0001-8993-371X FU Fundecitrus; Fapesp [2005/00718-2, 2007/55013-9]; CNPq [578049/2008-2]; Citrus Research and Development Foundation FX This work was financially supported by Fundecitrus, Fapesp (projects 2005/00718-2 and 2007/55013-9), CNPq (project 578049/2008-2), and Citrus Research and Development Foundation (project 8). R. B. Bassanezi, P. T. Yamamoto, L. Amorim, and A. Bergamin Filho have a fellowship from CNPq. We thank N. I. Barrancos from Sao Jose Farm; J. L. A. Rodrigues and F. E. A. Tersi from Cambuhy Agricola Ltd. for the experimental areas; R. A. Santos, P. H. Sperandio, M. A. Jorge, L. Pereira, C. N. Moreira, P. C. G. Basso, A. J. R. da Silva, W. M. Santos, M. A. B. Lima, C. D. Felix, J. C. dos Santos, and A. D. Campagne for field assistance; and G. H. Rodrigues and J. M. Martins for laboratory assistance. NR 23 TC 13 Z9 14 U1 1 U2 42 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 789 EP 796 DI 10.1094/PDIS-03-12-0314-RE PG 8 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300012 ER PT J AU Bock, CH Wood, BW van den Bosch, F Parnell, S Gottwald, TR AF Bock, Clive H. Wood, Bruce W. van den Bosch, Frank Parnell, Stephen Gottwald, Tim R. TI The Effect of Horsfall-Barratt Category Size on the Accuracy and Reliability of Estimates of Pecan Scab Severity SO PLANT DISEASE LA English DT Article ID DISEASE ASSESSMENT; CITRUS CANKER; VISUAL ESTIMATION; IMAGE-ANALYSIS; PRECISION; SYMPTOMS; BLIGHT AB Pecan scab (Fusicladium effusum) is a destructive pecan disease. Disease assessments may be made using interval-scale-based methods or estimates of severity to the nearest percent area diseased. To explore the effects of rating method-Horsfall-Barratt (H-B) scale estimates versus nearest percent estimates (NPEs)-on the accuracy and reliability of severity estimates over different actual pecan scab severity ranges on fruit valves, raters assessed two cohorts of images with actual area (0 to 6, 6(+) to 25%, and 25(+) to 75%) diseased. Mean estimated disease within each actual disease severity range varied substantially. Means estimated by NPE within each actual disease severity range were not necessarily good predictors of the H-B scale estimate at <25% severity. H-B estimates by raters most often placed severity in the wrong category compared with actual disease. Measures of bias, accuracy, precision, and agreement using Lin's concordance correlation depended on the range of actual severity, with improvements increasing with actual disease severity category (from 0 to 6 through 25(+) to 75%); however, the improvement was unaffected by the H-B assessments. Bootstrap analysis indicated that NPEs provided either equally good or more accurate and precise estimate of disease compared with the H-B scale at severities of 25(+) to 75%. Inter-rater reliability using NPEs was greater at 25(+) to 75% actual disease severity compared with using the H-B scale. Using NPEs compared with the H-B scale will more often result in more precise and accurate estimates of pecan scab severity, particularly when estimating actual disease severities of 25(+) to 75%. C1 [Bock, Clive H.; Wood, Bruce W.] USDA ARS, SEFTNRL, Byron, GA 31008 USA. [van den Bosch, Frank; Parnell, Stephen] Rothamsted Res, Harpenden AL5 2JQ, Herts, England. [Gottwald, Tim R.] USDA ARS USHRL, Ft Pierce, FL 34945 USA. RP Bock, CH (reprint author), USDA ARS, SEFTNRL, Byron, GA 31008 USA. EM clive.bock@ars.usda.gov RI parnell, stephen/I-7682-2015 OI parnell, stephen/0000-0002-2625-4557 NR 35 TC 7 Z9 7 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 797 EP 806 DI 10.1094/PDIS-08-12-0781-RE PG 10 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300013 ER PT J AU Molnar, TJ Walsh, E Capik, JM Sathuvalli, V Mehlenbacher, SA Rossman, AY Zhang, N AF Molnar, Thomas J. Walsh, Emily Capik, John M. Sathuvalli, Vidyasagar Mehlenbacher, Shawn A. Rossman, Amy Y. Zhang, Ning TI A Real-Time PCR Assay for Early Detection of Eastern Filbert Blight SO PLANT DISEASE LA English DT Article ID ANISOGRAMMA-ANOMALA; EUROPEAN HAZELNUT; PACIFIC-NORTHWEST; CORYLUS-AVELLANA; NEW-JERSEY; RESISTANCE; MARKERS; OREGON; INOCULATION; ACCESSIONS AB Eastern filbert blight (EFB) is a devastating disease of European hazelnut, Corylus avellana, which causes economic losses in Oregon, where 99% of the U.S. crop is produced. The causal fungus, Anisogramma anomala, is native to eastern North America, where it is found associated with the American hazelnut (C. americana). Although C. americana is tolerant, EFB causes cankers, branch dieback, and death of C. avellana. Detection and identification of A. anomala is time consuming using conventional methods because the fungus can only be cultured from sporulating perithecia and the disease symptoms and signs only show 12 to 16 months after infection. In this study, a TaqMan real-time polymerase chain reaction (PCR) assay based on a ribosomal DNA internal transcribed spacer was developed for A. anomala. The assay was validated with multiple isolates of A. anomala, closely related species, common environmental microorganisms, and over 100 C. avellana samples. The real-time PCR assay detected as low as 0.12 pg of A. anomala genomic DNA, and positively diagnosed EFB on 82% of asymptomatic plants as early as 15 weeks from infection. The real-time PCR assay is more sensitive and faster than traditional diagnostic methods. It can facilitate hazelnut breeding and disease management by early and accurate diagnosis of EFB. C1 [Molnar, Thomas J.; Walsh, Emily; Capik, John M.; Zhang, Ning] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Sathuvalli, Vidyasagar; Mehlenbacher, Shawn A.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Sathuvalli, Vidyasagar] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [Rossman, Amy Y.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Zhang, Ning] Rutgers State Univ, Dept Biochem & Microbiol, New Brunswick, NJ 08901 USA. RP Zhang, N (reprint author), Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. EM zhang@aesop.rutgers.edu RI Zhang, Ning/K-3046-2012 OI Zhang, Ning/0000-0003-0755-2505 FU New Jersey Agricultural Experiment Station; Rutgers Center for Turfgrass Science; United Stated Department of Agriculture (USDA)-NIFA; USDA-NIFA Specialty Crops Research Initiative [2009-51181-06028] FX This work was funded by the New Jersey Agricultural Experiment Station, the Rutgers Center for Turfgrass Science, Hatch funds provided by the United Stated Department of Agriculture (USDA)-NIFA, and the USDA-NIFA Specialty Crops Research Initiative Competitive Grant 2009-51181-06028. We thank the anonymous reviewers whose comments helped to improve this article. NR 53 TC 2 Z9 2 U1 1 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 813 EP 818 DI 10.1094/PDIS-11-12-1041-RE PG 6 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300015 ER PT J AU Rouse, MN Griffey, CA Brooks, WS AF Rouse, M. N. Griffey, C. A. Brooks, W. S. TI First Detection of Puccinia hordei Virulence to Barley Leaf Rust Resistance Gene Rph3 and Combination with Virulence to Rph7 in North America SO PLANT DISEASE LA English DT News Item C1 [Rouse, M. N.] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Griffey, C. A.; Brooks, W. S.] Virginia Polytech Inst & State Univ, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. RP Rouse, MN (reprint author), ARS, USDA, Cereal Dis Lab, 1551 Lindig St, St Paul, MN 55108 USA. RI Rouse, Matthew/G-5474-2011 NR 4 TC 0 Z9 0 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 838 EP 838 DI 10.1094/PDIS-08-12-0785-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300027 ER PT J AU Wang, MN Chen, XM AF Wang, M. N. Chen, X. M. TI First Report of Oregon Grape (Mahonia aquifolium) as an Alternate Host for the Wheat Stripe Rust Pathogen (Puccinia striiformis f. sp tritici) Under Artificial Inoculation SO PLANT DISEASE LA English DT News Item C1 [Wang, M. N.; Chen, X. M.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X. M.] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Wang, MN (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 3 TC 13 Z9 15 U1 1 U2 20 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 839 EP 839 DI 10.1094/PDIS-09-12-0864-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300030 ER PT J AU Johnson, DA Hulbert, S Demoz, B Fernando, WGD Paulitz, T AF Johnson, D. A. Hulbert, S. Demoz, B. Fernando, W. G. D. Paulitz, T. TI First Report of Blackleg Caused by Leptosphaeria maculans on Canola in Idaho SO PLANT DISEASE LA English DT News Item C1 [Johnson, D. A.; Hulbert, S.] Washington State Univ, Dept Pathol, Pullman, WA 99164 USA. [Demoz, B.; Fernando, W. G. D.] Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada. [Paulitz, T.] ARS, USDA, Pullman, WA 99164 USA. RP Johnson, DA (reprint author), Washington State Univ, Dept Pathol, Pullman, WA 99164 USA. OI Paulitz, Timothy/0000-0002-8885-3803 NR 2 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 842 EP 842 DI 10.1094/PDIS-10-12-0956-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300037 ER PT J AU Testen, AL McKemy, JM Backman, PA AF Testen, A. L. McKemy, J. M. Backman, P. A. TI First Report of Ascochyta Leaf Spot of Quinoa Caused by Ascochyta sp in the United States SO PLANT DISEASE LA English DT News Item ID CHENOPODIUM-QUINOA C1 [Testen, A. L.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. [McKemy, J. M.] Natl Identificat Serv, USDA, APHIS, PPQ, Beltsville, MD 20705 USA. [Backman, P. A.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. RP Testen, AL (reprint author), Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. NR 4 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUN PY 2013 VL 97 IS 6 BP 844 EP 844 DI 10.1094/PDIS-11-12-1008-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 148IF UT WOS:000319237300042 ER PT J AU Miller, RS Farnsworth, ML Malmberg, JL AF Miller, Ryan S. Farnsworth, Matthew L. Malmberg, Jennifer L. TI Diseases at the livestOck-wildlife interface: Status, challenges, and opportunities in the United States SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Livestock wildlife interface Agriculture; Adaptive management; Mutual transmission; Wildlife diseases; Zoonoses ID WHITE-TAILED DEER; CHRONIC WASTING DISEASE; EASTERN EQUINE ENCEPHALITIS; BUFFALO-NATIONAL-PARK; MYCOBACTERIUM-TUBERCULOSIS COMPLEX; GREATER YELLOWSTONE ECOSYSTEM; EPIZOOTIC HEMORRHAGIC-DISEASE; EMERGING INFECTIOUS-DISEASES; FINCHES CARPODACUS-MEXICANUS; TICK BOOPHILUS-MICROPLUS AB In the last half century, significant attention has been given to animal diseases; however, our understanding of disease processes and how to manage them at the livestock-wildlife interface remains limited. In this study, we conduct a systematic review of the scientific literature to evaluate the status of diseases at the livestock-wildlife interface in the United States. Specifically, the goals of the literature review were three fold: first to evaluate domestic animal diseases currently found in the United States where wildlife may play a role; second to identify critical issues faced in managing these diseases at the livestock-wildlife interface; and third to identify potential technical and policy strategies for addressing these issues. We found that of the 86 avian, ruminant, swine, poultry, and lagomorph diseases that are reportable to the World Organization for Animal Health (OIE), 53 are present in the United States; 42 (79%) of these have a putative wildlife component associated with the transmission, maintenance, or life cycle of the pathogen; and 21 (40%) are known to be zoonotic. At least six of these reportable diseases bovine tuberculosis, paratuberculosis, brucellosis, avian influenza, rabies, and cattle fever tick (vector control) have a wildlife reservoir that is a recognized impediment to eradication in domestic populations. The complex nature of these systems highlights the need to understand the role of wildlife in the epidemiology, transmission, and maintenance of infectious diseases of livestock. Successful management or eradication of these diseases will require the development of cross-discipline and institutional collaborations. Despite social and policy challenges, there remain opportunities to develop new collaborations and new technologies to mitigate the risks posed at the livestock-wildlife interface. Published by Elsevier B.V. C1 [Miller, Ryan S.; Farnsworth, Matthew L.; Malmberg, Jennifer L.] USDA, APHIS, Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. RP Miller, RS (reprint author), USDA, APHIS, Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. EM Ryan.Miller@rsmiller.net OI Miller, Ryan/0000-0003-3892-0251 NR 160 TC 36 Z9 38 U1 11 U2 108 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 J9 PREV VET MED JI Prev. Vet. Med. PD JUN 1 PY 2013 VL 110 IS 2 BP 119 EP 132 DI 10.1016/j.prevetmed.2012.11.021 PG 14 WC Veterinary Sciences SC Veterinary Sciences GA 147OM UT WOS:000319176900004 PM 23254245 ER PT J AU Hirschman, J Chriqui, JF AF Hirschman, Jay Chriqui, Jamie F. TI School food and nutrition policy, monitoring and evaluation in the USA SO PUBLIC HEALTH NUTRITION LA English DT Article DE School food; Public policy; Monitoring and evaluation; School nutrition ID SUGAR-SWEETENED BEVERAGES; COMPETITIVE FOOD; ELEMENTARY-SCHOOLS; PUBLIC-SCHOOLS; JUNK FOOD; IMPLEMENTATION; STATE; AVAILABILITY; CONSUMPTION; STUDENTS AB Objective: To provide an overview of school food and nutrition monitoring from 1980 to the Healthy Hunger-Free Kids Act of 2010 and data on school food availability in the USA. Design: A review of the history of school food and nutrition policy, monitoring and evaluation efforts in the USA over the past three decades. Setting: USA. Subjects: School food service, school districts and schools nationwide. Results: The school food environment in the USA is governed by a patchwork of federal, state and local laws and policies. The federal government has primary authority over the school meal programmes and has recently issued updated regulations governing the food and nutrient requirements for meals sold or served through the National School Lunch and School Breakfast Programs. Competitive foods (i.e. foods and beverages sold/served outside the meal programmes) are governed primarily by state and district laws and policies, although new federal regulations are expected to set minimum standards in this area. The USA has a long history of data monitoring and evaluation funded by government and private foundations which has enabled decision makers to monitor progress and opportunities to improve the foods and beverages made available to students in school. Conclusions: School food-related monitoring and evaluation research has been highly influential in influencing legislation and policy, leading to improvements in the foods and beverages available to children at school as part of planned meals and individual items sold outside the meal programmes. The lessons learned from the US experience provide insights that may be valuable for implementation, monitoring and evaluation of school food programmes in other countries. C1 [Hirschman, Jay] Food & Nutr Serv, USDA, Alexandria, VA 22302 USA. [Chriqui, Jamie F.] Univ Illinois, Inst Hlth Res & Policy, Chicago, IL USA. RP Hirschman, J (reprint author), Food & Nutr Serv, USDA, 3101 Pk Ctr Dr, Alexandria, VA 22302 USA. EM Jay.Hirschman@fns.usda.gov NR 52 TC 11 Z9 11 U1 7 U2 85 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD JUN PY 2013 VL 16 IS 6 BP 982 EP 988 DI 10.1017/S1368980012004144 PG 7 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA 148BW UT WOS:000319218500005 PM 23006629 ER PT J AU Cardamone, JM AF Cardamone, Jeanette M. TI Keratin sponge/hydrogel II: Active agent delivery SO TEXTILE RESEARCH JOURNAL LA English DT Article DE keratin; sponge/hydrogel; riboflavin delivery/diffusion drug release ID SOLUTE RELEASE; DRUG-RELEASE; IN-VITRO; RIBOFLAVIN; HYDROGELS; POLYMERS; CULTIVATION; MECHANISMS; SCAFFOLDS; BINDING AB Keratin sponge/hydrogels, described in Part I as the products of oxidation and reduction hydrolysis of fine and coarse wool fibers, were examined as excipient-platforms for the delivery of riboflavin. By absorption through well-defined pores and networks, saturated moisture equilibrium was reached within one hour with swelling ratios of 3.7-5.7. Riboflavin uptake by the four different keratin sponge/hydrogels was from 27.9 to 224 A mu g. Within 60 minutes, reductive keratin sponge/hydrogels from fine and coarse wool, and the oxidative species from fine wool, released 100% riboflavin at 1.58-1.73% per minute and oxidative sponge/hydrogel from coarse wool released 48% riboflavin at 0.8% riboflavin per minute. Rates of riboflavin release were faster within the initial 10 minutes where reductive sponge/hydrogels released 80-84% riboflavin at 8.0-8.4% per minute and oxidative sponge/hydrogels released 26-63% riboflavin at 2.6-6.3% per minute. Riboflavin delivery at timed intervals was examined with the Korsmeyer-Peppas mathematical model for fractional release. Release patterns followed quasi-Fickian diffusion of the drug by transport through channels and voids. All keratin sponge/hydrogels functioned as site-specific agents with two-stage delivery where immediate release of riboflavin was followed by a period of prolonged, lower dosage release. Swelling studies, the mobility of water, and the behavior of riboflavin release favorably framed keratin sponge/hydrogels as microfluidic devices with implied applications for advanced biomedical and biotechnological applications. C1 USDA, Wyndmoor, PA 19038 USA. RP Cardamone, JM (reprint author), USDA, 600 East Mermaid Ln, Wyndmoor, PA 19038 USA. EM jan.cardamone@ars.usda.gov NR 41 TC 4 Z9 4 U1 2 U2 21 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 J9 TEXT RES J JI Text. Res. J. PD JUN PY 2013 VL 83 IS 9 BP 917 EP 927 DI 10.1177/0040517512468815 PG 11 WC Materials Science, Textiles SC Materials Science GA 148CS UT WOS:000319221000004 ER PT J AU Liu, YL Foulk, J AF Liu, Yongliang Foulk, Jonn TI Potential of visible and near infrared spectroscopy in the determination of instrumental leaf grade in lint cottons SO TEXTILE RESEARCH JOURNAL LA English DT Article DE Cotton trash; leaf grade; cotton classification; visible and near infrared spectroscopy; NIR AB Existence of non-lint materials (or botanic trash) within commercial cotton bales degrades their market value, requires a further cleaning process, and compromises finished product quality. To meet the challenge of assessing the trash content, a number of approaches have been in practice. In the US, one term to assess the degree of trash amount is leaf grade, which was originally determined by qualified US Department of Agriculture's AMS cotton classers via a visual inspection procedure. Recently, the AMS has revised the protocol for cotton leaf grade classification, by replacing the classer's leaf determination with instrumental leaf measurement from cotton classification HVI (TM) system. In this study, visible/NIR spectra were acquired to explore the potential for the discrimination of cotton samples with various leaf grade categories. Seven-class classification models in different spectral regions were developed to optimize the identification efficiency. Results indicated that using the model in the 1105-1700 nm NIR region could reach an acceptable separation of similar to 95.0%, with a 89.9% correct identification in validation set and a 100% success in calibration set. Furthermore, factors of influencing the correct classification were discussed briefly. C1 [Liu, Yongliang] ARS, SRRC, USDA, New Orleans, LA 70124 USA. [Foulk, Jonn] FX Fibers LLC, Clemson, SC 29631 USA. RP Liu, YL (reprint author), ARS, Cotton Struct & Qual Res Unit, SRRC, USDA, New Orleans, LA 70124 USA. EM yongliang.liu@ars.usda.gov NR 20 TC 4 Z9 4 U1 0 U2 7 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 J9 TEXT RES J JI Text. Res. J. PD JUN PY 2013 VL 83 IS 9 BP 928 EP 936 DI 10.1177/0040517513478481 PG 9 WC Materials Science, Textiles SC Materials Science GA 148CS UT WOS:000319221000005 ER PT J AU Cai, YY Cui, XL Rodgers, J Thibodeaux, D Martin, V Watson, M Pang, SS AF Cai, Yiyun Cui, Xiaoliang Rodgers, James Thibodeaux, Devron Martin, Vikki Watson, Mike Pang, Su-Seng TI A comparative study of the effects of cotton fiber length parameters on modeling yarn properties SO TEXTILE RESEARCH JOURNAL LA English DT Article DE Cotton; fiber length measurement; yarn quality; spinning AB Fiber length is one of the key properties of cotton and has important influences on yarn production and yarn quality. Various parameters have been developed to characterize cotton fiber length in the past decades. This study was carried out to investigate the effects of these parameters and their combinations on yarn properties. Linear regression models with different numbers of fiber length parameters and their combinations were developed for predicting ring and open-end (OE) spun yarns' properties. The R-2 and Mallows' Cp plots of the models were compared for model selections. The results indicate that, for predicting a yarn property, a model usually involves more than three length parameters to achieve better prediction when considering the R-2 and Cp values. This may be because only one single length parameter cannot sufficiently represent fiber length characteristics. The results also show that the variations in fiber length distributions play important roles in predicting yarn properties, such as strength and irregularity. The best prediction models for the properties of different yarns (ring, OE) include different combinations of length parameters. Not all yarn properties can be well predicted by linear regression models with length parameters: other fiber properties (strength, micronaire, etc.) need to be included to further improve the models. C1 [Cai, Yiyun; Pang, Su-Seng] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Cui, Xiaoliang; Rodgers, James] SRRC ARS USDA, New Orleans, LA 70124 USA. [Thibodeaux, Devron] USDA ARS CQRS, Washington, DC USA. RP Cui, XL (reprint author), SRRC ARS USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM leon.cui@ars.usda.gov FU Cotton Incorporated FX This work was supported by Cotton Incorporated. NR 22 TC 4 Z9 4 U1 0 U2 13 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 J9 TEXT RES J JI Text. Res. J. PD JUN PY 2013 VL 83 IS 9 BP 961 EP 970 DI 10.1177/0040517512468821 PG 10 WC Materials Science, Textiles SC Materials Science GA 148CS UT WOS:000319221000008 ER PT J AU Cintron, MS Ingber, BF AF Cintron, Michael Santiago Ingber, Bruce F. TI Preliminary examination of the effects of relative humidity on the fracture morphology of cotton flat bundles SO TEXTILE RESEARCH JOURNAL LA English DT Article DE Cotton; tenacity; elongation; moisture; AFIS; HVI; fiber fracture; relative humidity AB A preliminary examination of the effects of relative humidity (RH) of three testing conditions on cotton fiber fracture morphology is presented herein. In addition, measurements of fiber moisture content, stelometer cotton flat bundle strength and elongation were collected at the three testing conditions. A general trend is observed for moisture content, strength and elongations measurements; testing in conditions with higher RH generally resulted in a progressive increase in moisture content, strength and elongation values. The morphology of broken fibers was also affected by the testing conditions. Fibers broken at high RH (i.e. 70 +/- 2 degrees F and 80 +/- 2% RH) showed a more frayed fracture where microfibril separation, a fracture pattern that suggests independent microfibril failure, was evident. In contrast, at standard conditions (i.e. 70 +/- 2 degrees F and 65 +/- 2%), fiber fractures were more granular (clean fractures), a reflection of a more unilateral breaking action. At low RH (i.e. 70 +/- 2 degrees F and 50 +/- 2%), fiber fractures exhibited a distorted granular pattern, with extended fracture breaks that did not exhibit microfibril separation. Our preliminary findings are of relevance to post-harvest moisture control efforts currently employed in industry and may contribute to larger efforts to understand the effects of the fracture and damage observed in cotton fiber properties. C1 [Cintron, Michael Santiago; Ingber, Bruce F.] ARS, SRRC, USDA, Washington, DC USA. RP Cintron, MS (reprint author), 1100 Robert L Lee Blvd, New Orleans, LA 70124 USA. EM michael.santiago@ars.usda.gov NR 17 TC 0 Z9 0 U1 1 U2 5 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0040-5175 J9 TEXT RES J JI Text. Res. J. PD JUN PY 2013 VL 83 IS 10 BP 1044 EP 1054 DI 10.1177/0040517512470194 PG 11 WC Materials Science, Textiles SC Materials Science GA 148CV UT WOS:000319221300005 ER PT J AU Blaker, KM Chaparro, JX Beckman, TG AF Blaker, Kendra M. Chaparro, Jose X. Beckman, Thomas G. TI Identification of QTLs controlling seed dormancy in peach (Prunus persica) SO TREE GENETICS & GENOMES LA English DT Article DE Peach; Seed dormancy; Quantitative trait loci; Stratification; Germination ID GENETIC-LINKAGE MAP; L. BATSCH; MICROSATELLITE MARKERS; HEAT REQUIREMENT; ALMOND; GERMINATION; STRATIFICATION; TEMPERATURE; GROWTH; PROPAGULES AB Dormancy is a condition that delays or inhibits growth in seed, vegetative buds, and floral buds. In peach, seed germination occurs when seed accumulate sufficient stratification and growing degree hours to break dormancy and begin growing. Correlations have been reported between mean seed stratification requirements and mean bud chilling requirements among Prunus families, but an individual seed's germination date and subsequent vegetative and floral bud break date are not correlated. Prior to this study, the genetic factors involved in regulating seed dormancy and their location on the peach genomic map were unknown. Segregating F-2 seed were collected from a high x low chill F-1 peach hybrid in 2005, 2006, and 2008. Germination date and growth habit was measured after the stratification requirement of the 2005 seed was fully met. The seed collected in 2006 and 2008 received varying amounts of stratification, which enabled data on stratification requirement, heat requirement, and growth habit to be collected. Genomic DNA was extracted from seedling leaf tissue and screened with SSR markers selected from the Prunus reference map at an average resolution of 20 cM. Seed dormancy quantitative trait loci (QTLs) were detected on G1, G4, G6/8, and G7. The QTLs detected on G6/8 and G7 were discovered in the same region as QTLs associated with floral bud chilling requirement and bloom time in peach. C1 [Blaker, Kendra M.; Chaparro, Jose X.] Univ Florida, Gainesville, FL 32611 USA. [Beckman, Thomas G.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Blaker, KM (reprint author), Univ Florida, Fifield Hall, Gainesville, FL 32611 USA. EM kblaker@ufl.edu NR 43 TC 5 Z9 5 U1 2 U2 41 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2013 VL 9 IS 3 BP 659 EP 668 DI 10.1007/s11295-012-0578-4 PG 10 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 143PF UT WOS:000318879000003 ER PT J AU Yang, JY Scascitelli, M Motilal, LA Sveinsson, S Engels, JMM Kane, NC Dempewolf, H Zhang, DP Maharaj, K Cronk, QCB AF Yang, Ji Yong Scascitelli, Moira Motilal, Lambert A. Sveinsson, Saemundur Engels, Johannes M. M. Kane, Nolan C. Dempewolf, Hannes Zhang, Dapeng Maharaj, Kamaldeo Cronk, Quentin C. B. TI Complex origin of Trinitario-type Theobroma cacao (Malvaceae) from Trinidad and Tobago revealed using plastid genomics SO TREE GENETICS & GENOMES LA English DT Article DE Theobroma cacao; Chloroplast; Microsatellites; Single nucleotide polymorphisms; Trinitario ID INTERNATIONAL COCOA GENEBANK; CHLOROPLAST MICROSATELLITES; MAXIMUM-LIKELIHOOD; PHYLOGENETIC TREES; GENETIC DIVERSITY; NUCLEAR; DNA; L.; POPULATION; GERMPLASM AB Trinidad and Tobago has a long history of producing high-quality cacao (Theobroma cacao L.). Cacao genotypes in Trinidad and Tobago are of a highly distinctive kind, the so-called "Trinitario" cultivar group, widely considered to be of elite quality. The origin of Trinitario cacao is unclear, although it is generally considered to be of hybrid origin. We used massive parallel sequencing to identify polymorphic plastidic single nucleotide polymorphisms (cpSNPs) and polymorphic plastidic simple sequence repeats (cpSSRs) in order to determine the origin of the Trinitario cultivar group by comparing patterns of polymorphism to a reference set of ten completely sequenced chloroplast genomes (nine T. cacao and one outgroup, T. grandiflorum (Willd. ex Spreng.) Schum). Only three cpSNP haplotypes were present in the Trinitario cultivars sampled, each highly distinctive and corresponding to reference genotypes for the Criollo (CRI), Upper Amazon Forastero (UAF) and Lower Amazon Forastero (LAF) varietal groups. These three cpSNP haplotypes likely represent the founding lineages of cacao to Trinidad and Tobago. The cpSSRs were more variable with eight haplotypes, but these clustered into three groups corresponding to the three cpSNP haplotypes. The most common haplotype found in farms of Trinidad and Tobago was LAF, followed by UAF and then CRI. We conclude that the Trinitario cultivar group is of complex hybrid origin and has derived from at least three original introduction events. C1 [Yang, Ji Yong; Scascitelli, Moira; Sveinsson, Saemundur; Kane, Nolan C.; Dempewolf, Hannes; Cronk, Quentin C. B.] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Motilal, Lambert A.] Univ W Indies, Cocoa Res Unit, St Augustine, Trinidad, Trinid & Tobago. [Engels, Johannes M. M.] Biodivers Int, I-00057 Maccarese Rome, Italy. [Zhang, Dapeng] USDA ARS, SPCL, Beltsville, MD 20705 USA. [Maharaj, Kamaldeo] Minist Agr Food Prod Land & Marine Resources Affa, Cent Expt Stn, Centeno, Trinid & Tobago. RP Yang, JY (reprint author), Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada. EM albertoyang@yahoo.com RI Sveinsson, Saemundur/F-8262-2011; KANE, NOLAN/B-7652-2016 OI KANE, NOLAN/0000-0001-9133-6543 FU World Bank; NSERC (Canada) FX We thank the World Bank for a Development Marketplace grant awarded to QC, HD and Johannes Engels and coordinated by Bioversity International. The Staff of Cocoa Research, Centeno, Trinidad is highly acknowledged for identifying and collecting leaves from the farmers' cacao trees, sometimes under very challenging conditions. We thank Chris Grassa for analytical assistance, and Brian Irish (USDA, ARS) and Kyle Wallick (USBG) for providing leaf samples. Stephen Pinney (USDA, ARS) and Kasey Gordon (CRU) are thanked for assistance with DNA extractions. Jon Armstrong and Jarret Glasscock (Cofactor Genomics, St. Louis) provided valuable sequencing assistance. We also gratefully acknowledge the laboratory support from an NSERC (Canada) discovery grant to Quentin Cronk. NR 56 TC 6 Z9 6 U1 1 U2 18 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2013 VL 9 IS 3 BP 829 EP 840 DI 10.1007/s11295-013-0601-4 PG 12 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 143PF UT WOS:000318879000017 ER PT J AU Al-Chokhachy, R Ray, AM Roper, BB Archer, E AF Al-Chokhachy, Robert Ray, Andrew M. Roper, Brett B. Archer, Eric TI Exotic Plant Colonization and Occupancy Within Riparian Areas of the Interior Columbia River and Upper Missouri River Basins, USA SO WETLANDS LA English DT Article DE Exotic plants; Occupancy model; Riparia; Headwaters ID WESTERN NORTH-AMERICA; CHEATGRASS BROMUS-TECTORUM; CLIMATE-CHANGE; SPOTTED KNAPWEED; CANADA THISTLE; UNITED-STATES; GREAT-BASIN; DETECTION PROBABILITIES; CENTAUREA-MACULOSA; FESCUE GRASSLANDS AB Exotic plant invasions into riparia often result in shifts in vegetative composition, altered stream function, and cascading effects to biota at multiple scales. Characterizing the distribution patterns of exotic plants is an important step in directing targeted research to identify mechanisms of invasion and potential management strategies. In this study, we employed occupancy models to examine the associations of landscape, climate, and disturbance attributes with the colonization and occupancy patterns for spotted knapweed (Centaurea stoebe L.), Canada thistle (Cirsium arvense L., Scop.), and cheatgrass (Bromus tectorum L.) in the riparia of headwater streams (n = 1,091) in the Interior Columbia River and Upper Missouri River Basins. We found relatively low occupancy rates for cheatgrass (0.06, SE = 0.02) and spotted knapweed (0.04, SE = 0.01), but moderate occupancy of Canada thistle (0.28, SE = 0.05); colonization rates were low across all species (< 0.01). We found the distributions of spotted knapweed, Canada thistle, and cheatgrass to exhibit significant associations with both ambient climate conditions and anthropogenic and natural disturbances. We attribute the low to moderate occupancy and colonization rates to the relatively remote locations of our sample sites within headwater streams and urge consideration of means to prevent further invasions. C1 [Al-Chokhachy, Robert] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Ray, Andrew M.] Natl Pk Serv, Greater Yellowstone Network, Bozeman, MT 59715 USA. [Roper, Brett B.; Archer, Eric] US Forest Serv, USDA, Logan, UT USA. RP Al-Chokhachy, R (reprint author), US Geol Survey, Northern Rocky Mt Sci Ctr, 2327 Univ Way,Suite 2, Bozeman, MT 59715 USA. EM ral-chokhachy@usgs.gov FU U.S. Geological Survey Mendenhall Fellowship; U.S. Forest Service region 1; U.S. Forest Service region 4; U.S. Forest Service region 6; BLM office in Oregon-Washington; BLM office in Idaho FX Funding for R. Al-Chokhachy was in part through the U.S. Geological Survey Mendenhall Fellowship Program. We would like to thank the extensive list of summer field technicians who collected the data for these analyses and A. Sepulveda (U.S. Geological Survey) for reviewing and contributing to earlier drafts of this manuscript. The U.S. Forest Service regions 1, 4, and 6 and BLM offices in Oregon-Washington and Idaho provided funding for this project. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 115 TC 3 Z9 4 U1 3 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 J9 WETLANDS JI Wetlands PD JUN PY 2013 VL 33 IS 3 BP 409 EP 420 DI 10.1007/s13157-013-0399-8 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 142LC UT WOS:000318797900003 ER PT J AU Brennan, EB AF Brennan, Eric B. TI Agronomic aspects of strip intercropping lettuce with alyssum for biological control of aphids SO BIOLOGICAL CONTROL LA English DT Article DE Intercropping; Organic farming; Biological control of aphids; Lettuce; Alyssum; Vegetable production ID NASONOVIA-RIBISNIGRI; HABITAT MANAGEMENT; HOVERFLIES DIPTERA; ORGANIC LETTUCE; SWEET ALYSSUM; CENTRAL COAST; CALIFORNIA; PLANTS; LONGEVITY; CONSERVATION AB Organic lettuce growers in California typically use insectary strips of alyssum (Lobularia maritima (L.) Desv.) to attract hoverflies (Syrphidae) that provide biological control of aphids. A two year study with transplanted organic romaine lettuce in Salinas, California investigated agronomic aspects of lettuce monoculture and lettuce-alyssum strip intercropping on beds in replacement intercropping treatments where alyssum transplants replaced 2 to 8% of the lettuce transplants, and in additive intercropping treatments where alyssum transplants were added to the standard lettuce density without displacing lettuce transplants. Alyssum and lettuce dry matter (DM) were determined at lettuce maturity. Alyssum transplants produced less shoot DM in the additive than in the replacement intercropping treatments. The number of open inflorescences of alyssum increased with alyssum DM, and among treatments ranged from 2 to15 inflorescences per lettuce head. Compared with monoculture lettuce, lettuce heads on intercropped beds were slightly smaller and had lower nitrogen concentrations in the both additive treatments and in some replacement treatments. This research provides the first information on a novel additive intercropping approach to provide alyssum floral resources for biological control of lettuce aphids, and suggests that this approach may be a more land-efficient particularly for producing smaller lettuce heads for romaine hearts or for markets with less strict size requirements. Additional research is needed to determine if the increased competition between alyssum and lettuce in additive intercropping would reduce lettuce yields for wholesale markets with larger head size requirements. Practical aspects of implementing the various intercropping arrangements and alternatives are discussed. C1 ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. RP Brennan, EB (reprint author), ARS, USDA, US Agr Res Stn, 1636 East Alisal St, Salinas, CA 93905 USA. EM Eric.Brennan@ars.usda.gov NR 43 TC 7 Z9 7 U1 8 U2 74 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2013 VL 65 IS 3 BP 302 EP 311 DI 10.1016/j.biocontrol.2013.03.017 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 145CO UT WOS:000318991800002 ER PT J AU Ugine, TA Wraight, SP Sanderson, JP AF Ugine, Todd A. Wraight, Stephen P. Sanderson, John P. TI Microbial biological control potential of three strains of Beauveria bassiana s. l. against greenhouse shore fly Scatella tenuicosta: Assessment of virulence, mass production capacity, and effects on shore fly reproduction SO BIOLOGICAL CONTROL LA English DT Article DE Shore fly; Scatella tenuicosta; Fecundity; Entomopathogenic fungus; Beauveria bassiana; Virulence ID WESTERN FLOWER THRIPS; FRANKLINIELLA-OCCIDENTALIS; METARHIZIUM-ANISOPLIAE; AERIAL TRANSMISSION; FLIES; EPHYDRIDAE; FECUNDITY; STAGNALIS; DIPTERA AB The microbial biological control potential of three strains of Beauveria bassiana sensu lato originally isolated from the shore fly Scatella tenuicosta (Diptera: Ephydridae) was assessed in a series of laboratory bioassays. Comparisons were made to two commercially-available strains of B. bassiana. Two of the shore fly strains proved 27-67 times more virulent than the commercial strains in terms of LC50 (14-17 vs. 458-942 conidia/mm(2)) and killed shore flies more rapidly. B. bassiana s. l. strain ST1 exhibited a mass production capacity comparable to the commercial B. bassiana stain GHA, producing 2.8 x 10(12) conidia/kg barley-based solid substrate in ventilated mushroom spawn bags. The shore fly strains of Beauveria sporulated on a higher percentage of killed adult shore flies and produced substantially greater numbers of conidia per cadaver than the commercial strains, indicating that these pathogens are well adapted to this host. Female shore flies treated with strain ST1 survived for only 5 days, with longevity being reduced by 8-10 days compared to control insects. This reduction in survival had a large impact on total lifetime egg production, reducing it by 78-88%, depending on the time of treatment relative to the pre-oviposition period. However, fungal growth within infected female shore flies had no effect on egg production or egg viability until the day before the flies succumbed to mycosis (day 4 post-inoculation). As a consequence, the intrinsic rate of shore fly population increase and population doubling time were little affected by fungal infection (0.4357 vs. 0.4152 and 1.6 vs. 1.7 days for control vs. Beauveria-treated populations, respectively). These findings underscore the challenges involved with use of slow-acting pathogens for control of highly fecund greenhouse pests and the fundamental necessity of integrating these agents into integrated pest management systems. (c) 2013 Elsevier Inc. All rights reserved. C1 [Ugine, Todd A.; Sanderson, John P.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Wraight, Stephen P.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Ugine, TA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM tau2@cornell.edu FU USDA-ARS Plant Protection Research Unit; Cornell University Department of Entomology, Ithaca, NY [58-1907-4-447]; USDA-ARS, as part of the Floriculture and Nursery Research Initiative FX This research was funded in part through a Specific Cooperative Agreement between the USDA-ARS Plant Protection Research Unit and the Cornell University Department of Entomology, Ithaca, NY (Specific Cooperative Agreement #58-1907-4-447) funded by the USDA-ARS, as part of the Floriculture and Nursery Research Initiative. This paper reports the results of research only. Mention of a proprietary product does not constitute a recommendation or endorsement for its use by the US Department of Agriculture. NR 25 TC 2 Z9 2 U1 2 U2 39 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUN PY 2013 VL 65 IS 3 BP 348 EP 356 DI 10.1016/j.biocontrol.2013.03.014 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 145CO UT WOS:000318991800007 ER PT J AU Yee, WL AF Yee, Wee L. TI Soil moisture and relative humidity effects during postdiapause on the emergence of western cherry fruit fly (Diptera: Tephritidae) SO CANADIAN ENTOMOLOGIST LA English DT Article ID HOST-PLANT USE; APPLE MAGGOT; DESICCATION RESISTANCE; RHAGOLETIS-POMONELLA; WASHINGTON-STATE; ADULT EMERGENCE; PUPAL SURVIVAL; FIXED-POINTS; WATER-LOSS; HYMENOPTERA AB Pupae of western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), from Kennewick and Roslyn in Washington State, United States of America, were subjected to different soil and air moisture conditions to test the hypotheses that adult emergence from dry and moist soil is similar and is greater and occurs earlier at high than low relative humidities (RHs). Adult fly emergence from 0-57% saturated loam soil did not differ in Kennewick (64.8-75.1%) and Roslyn (81.7-86.6%) populations. Emergence did not occur from 76% saturated loam soil. In a RH experiment using dry soil, emergence of Kennewick flies was highest at 97% RH (76.8%), intermediate at 12-74% RH (66.4-69.6%), and lowest at 3% RH (58.0%). Emergence of Roslyn flies was higher at 97% RH (85.6%) than 3% (69.2%) and 12% RH (74.3%), but it did not differ from that at 33% (76.9%) and 74% RH (79.4%). Flies emerged similar to 2-3 days earlier at 97% RH than at 3-12% RH. An unnaturally low RH of 3% caused more flies to be deformed than higher RHs. High emergence of R. indifferens in dry and moist conditions likely contributes to its presence in a wide range of native and nonnative habitats. C1 ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov NR 34 TC 3 Z9 3 U1 0 U2 17 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2013 VL 145 IS 3 BP 317 EP 326 DI 10.4039/tce.2013.7 PG 10 WC Entomology SC Entomology GA 143ZM UT WOS:000318908400008 ER PT J AU Handiseni, M Brown, J Zemetra, R Mazzola, M AF Handiseni, Maxwell Brown, Jack Zemetra, Robert Mazzola, Mark TI Effect of Brassicaceae seed meals with different glucosinolate profiles on Rhizoctonia root rot in wheat SO CROP PROTECTION LA English DT Article DE Brassica napus; Brassica juncea; Sinapis alba; Seed meal; Rhizoctonia solani AG-8; Glucosinolate; Biopesticide ID PACIFIC-NORTHWEST; REDUCED TILLAGE; BARE PATCH; AMENDMENT; REGISTRATION; SUPPRESSION; BARLEY; BIOFUMIGATION; INHIBITION; DISEASES AB The soil-borne pathogen Rhizoctonia solani AG 8 causes major yield losses in wheat (Triticum aestivum. L) production worldwide. Plant tissues of Brassicaceae species contain glucosinolates that are hydrolyzed in the presence of the enzyme myrosinase into products with pesticidal properties. Growth chamber studies were conducted to determine the effect of the Brassicaceae seed meals (SMs) from Brassica juncea, Brassica napus and Sinapis alba on the suppression of the R. solani AG 8 infection of winter wheat. Pasteurized sandy soils were amended with intact and denatured SMs of rape seed and mustard at a rate of 0.5% by soil weight. Regardless of the glucosinolate type and content, all intact and denatured Brassicaceae significantly reduced the infection of winter wheat seedlings by R. solani AG 8 compared to the un-amended control. However, soils amended with S. alba SMs had the lowest severity of Rhizoctonia root rot relative to other amended soils. Phytotoxicity arising from the use of Brassicaceae SMs was observed particularly in soils amended with high glucosinolate-containing SMs. These studies demonstrate that Brassicaceae SMs can be used to manage disease caused by R. solani AG-8. However, future studies will need to focus on strategies for diminishing the crop growth-reducing effects associated with Brassicaceae SM amendment to fully maximize these fungicidal benefits. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Handiseni, Maxwell; Brown, Jack] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. [Zemetra, Robert] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Mazzola, Mark] USDA ARS, Wenatchee, WA 98801 USA. RP Handiseni, M (reprint author), Texas A&M Univ, Dept Plant Pathol & Microbiol, 120 Peterson,2132 TAMU, College Stn, TX 77843 USA. EM mhandiseni@vandals.uidaho.edu FU Eco-Energy Ltd. FX We thank Tim Paulitz for granting permission to use the WinRHIZO software developed in his laboratory and Nathalie Walters and Sheila Ivanov for their technical assistance. This project is partially supported by a grant from Eco-Energy Ltd. NR 33 TC 13 Z9 15 U1 3 U2 64 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD JUN PY 2013 VL 48 BP 1 EP 5 DI 10.1016/j.cropro.2013.01.006 PG 5 WC Agronomy SC Agriculture GA 146NG UT WOS:000319097200001 ER PT J AU Showler, AT Cook, SC Abrigo, V AF Showler, Allan T. Cook, Steven C. Abrigo, Veronica TI Transgenic Bt corn varietal resistance against the Mexican rice borer, Eoreuma loftini (Dyar) (Lepidoptera: Crambidae) and implications to sugarcane SO CROP PROTECTION LA English DT Article DE Bacillus thuringiensis; Cultivar; Integrated pest management; Saccharum; Trap crop; Variety; Zea mays ID RIO-GRANDE VALLEY; POPULATION-DYNAMICS; PYRALIDAE; TEXAS; MANAGEMENT; LOUISIANA; MAIZE AB The Mexican rice borer, Eoreuma loftini (Dyar), attacks crops including corn, Zea mays L., rice, Oryza sativa L., sorghum, Sorghum bicolor (L.) Moench, and sugarcane, Saccharum spp. Strongly resistant varieties of any kind, native or otherwise, have not been identified. A field plot corn variety test using two transgenic Bt varieties, Pioneer 31G71, expressing the Cry1F insecticidal protein, and Golden Acres 28V81, expressing the Cry1A.105, Cry2Ab2, and Cry3Bb1 insecticidal proteins, and two non-Bt controls, Dekalb DKC 69-72 and BH Genetics 9050, all four commonly grown in the Lower Rio Grande Valley of Texas, showed that, although oviposition preference was not affected, 28V81 resisted larval stalk boring to the extent that Mexican rice borer injury was almost non-existent. Pioneer 31G71 was infested nearly as much as the controls, but larval development to adulthood was reduced by approximate to 70%. Rearing larvae on 5, 50, 500, and 5000 mu g of corn leaf tissue per ml of artificial diet showed that, while the three lowest concentrations did not affect larval growth and development, the high concentration of 28V81 reduced survivorship to the pupal stage, decreased weight of 4-wk-old larvae, and prolonged development to pupation. Lower numbers of pheromone trap-captured adults at the edges of commercial Bt and non-Bt corn fields showed that populations were lower at the Bt cornfields, suggesting a lesser rate of adult production. Because corn is a preferred host plant over sugarcane, sorghum or rice, use of resistant transgenic Bt corn varieties will likely protect the crop from the substantial injury that can be caused by the pest. This study also suggests that Bt genes might result in similarly strong resistance when inserted in other vulnerable crops such as sugarcane. Published by Elsevier Ltd. C1 [Showler, Allan T.; Abrigo, Veronica] USDA ARS IFNRRU, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Cook, Steven C.] USDA ARS Honeybee Lab, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Showler, AT (reprint author), USDA ARS IFNRRU, Kika de la Garza Subtrop Agr Res Ctr, 2413 East Highway 83,Bldg 201, Weslaco, TX 78596 USA. EM allan.showler@ars.usda.gov NR 33 TC 5 Z9 5 U1 2 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 J9 CROP PROT JI Crop Prot. PD JUN PY 2013 VL 48 BP 57 EP 62 DI 10.1016/j.cropro.2013.02.007 PG 6 WC Agronomy SC Agriculture GA 146NG UT WOS:000319097200010 ER PT J AU Moore, DJP Trahan, NA Wilkes, P Quaife, T Stephens, BB Elder, K Desai, AR Negron, J Monson, RK AF Moore, David J. P. Trahan, Nicole A. Wilkes, Phil Quaife, Tristan Stephens, Britton B. Elder, Kelly Desai, Ankur R. Negron, Jose Monson, Russell K. TI Persistent reduced ecosystem respiration after insect disturbance in high elevation forests SO ECOLOGY LETTERS LA English DT Article DE Carbon balance; disturbance; ecosystem respiration; gross primary productivity; insect outbreak; lodgepole pine; mountain pine beetle; mountain West; subalpine forest ID MOUNTAIN PINE-BEETLE; SUB-ALPINE FOREST; INDUCED TREE MORTALITY; COLD-AIR DRAINAGE; SOIL RESPIRATION; CLIMATE-CHANGE; UNITED-STATES; EXOENZYME ACTIVITY; WOODY DEBRIS; EDDY FLUX AB Amid a worldwide increase in tree mortality, mountain pine beetles (Dendroctonus ponderosae Hopkins) have led to the death of billions of trees from Mexico to Alaska since 2000. This is predicted to have important carbon, water and energy balance feedbacks on the Earth system. Counter to current projections, we show that on a decadal scale, tree mortality causes no increase in ecosystem respiration from scales of several square metres up to an 84km2 valley. Rather, we found comparable declines in both gross primary productivity and respiration suggesting little change in net flux, with a transitory recovery of respiration 67years after mortality associated with increased incorporation of leaf litter C into soil organic matter, followed by further decline in years 810. The mechanism of the impact of tree mortality caused by these biotic disturbances is consistent with reduced input rather than increased output of carbon. C1 [Moore, David J. P.; Monson, Russell K.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Trahan, Nicole A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Wilkes, Phil] RMIT, Sch Math & Geospatial Sci, Melbourne, Vic 3001, Australia. [Quaife, Tristan] Univ Reading, Dept Meteorol, Reading RG6 6BB, Berks, England. [Quaife, Tristan] Univ Reading, Natl Ctr Earth Observat, Reading RG6 6BB, Berks, England. [Stephens, Britton B.] Natl Ctr Atmospher Res, Boulder, CO 80305 USA. [Elder, Kelly; Negron, Jose] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Desai, Ankur R.] Univ Wisconsin, Dept Atmospher & Ocean Sci, Madison, WI 53706 USA. [Monson, Russell K.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. RP Moore, DJP (reprint author), Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. EM davidjpmoore@email.arizona.edu RI Quaife, Tristan/C-1355-2008; Stephens, Britton/B-7962-2008; Moore, David/A-6268-2013; Desai, Ankur/A-5899-2008; OI Quaife, Tristan/0000-0001-6896-4613; Stephens, Britton/0000-0002-1966-6182; Desai, Ankur/0000-0002-5226-6041; Wilkes, Phil/0000-0001-6048-536X NR 47 TC 30 Z9 31 U1 1 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD JUN PY 2013 VL 16 IS 6 BP 731 EP 737 DI 10.1111/ele.12097 PG 7 WC Ecology SC Environmental Sciences & Ecology GA 145EE UT WOS:000318996400002 PM 23496289 ER PT J AU Randolph, KC AF Randolph, KaDonna C. TI Development history and bibliography of the US Forest Service crown-condition indicator for forest health monitoring SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Crown condition; Forest health monitoring; Forest survey history; Visual assessments AB Comprehensive assessment of individual-tree crown condition by the US Department of Agriculture, Forest Service, Forest Inventory and Analysis (FIA) Program has its origins in the concerns about widespread forest decline in Europe and North America that developed in the late 1970s and early 1980s. Programs such as the US National Acid Precipitation Assessment Program, US National Vegetation Survey, Canadian Acid Rain National Early Warning System, and joint US-Canadian North American Sugar Maple Decline Project laid the groundwork for the development of the US Forest Service crown-condition indicator. The crown-condition assessment protocols were selected and refined through literature review, peer review, and field studies in several different forest types during the late 1980s and early 1990s. Between 1980 and 2011, 126 publications relating specifically to the crown-condition indicator were added to the literature. The majority of the articles were published by the US Department of Agriculture, Forest Service or other State or Federal government agency, and more than half were published after 2004. C1 Forest Serv, USDA, Knoxville, TN 37919 USA. RP Randolph, KC (reprint author), Forest Serv, USDA, 4700 Old Kingston Pike, Knoxville, TN 37919 USA. EM krandolph@fs.fed.us NR 31 TC 5 Z9 5 U1 2 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD JUN PY 2013 VL 185 IS 6 BP 4977 EP 4993 DI 10.1007/s10661-012-2919-z PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA 138IV UT WOS:000318503100037 PM 23054283 ER PT J AU Engeman, RM Couturier, KJ Felix, RK Avery, ML AF Engeman, Richard M. Couturier, Kathy J. Felix, Rodney K., Jr. Avery, Michael L. TI Feral swine disturbance at important archaeological sites SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Animal damage; Conservation; Feral hog; Invasive species; Provenience; Stratigraphy; Sus scrofa ID FLORIDA AB Feral swine are well known as environmentally destructive invasive animals in many areas around the world, where they degrade native habitats, harm rare plant and animal species, damage agricultural interests, and spread disease. We provide the first quantification of their potential as agents of disturbance at archaeological sites. Our study was conducted in south-central Florida at Avon Park Air Force Range, a base comprising over 40,000 ha and containing many archaeological sites. To determine the identifiable prevalence of feral swine disturbance, we examined 36 sites registered with the Florida State Historic Preservation Office and also eligible for inclusion in the National Register of Historic Places (NRHP). Moreover, we evaluated the extent of swine disturbance at a prehistoric site of extraordinary significance to Florida's prehistory, "Dead Cow." Fifteen of the 36 NRHP-eligible sites (42 %) had some level of swine disturbance, including 14 of 30 (47 %) sites known to have artifacts within 20 cm of the surface (well within swine rooting depths). At the Dead Cow site, we documented disturbance at 74 % of shovel test points. Sites with shallow artifact depositions appeared highly vulnerable to disturbance by feral swine, threatening destruction of artifact stratigraphy and provenience. Our observations likely are a minimal representation of accumulated damage. These irreplaceable sites tell the area's land use story across the millennia. That they are under threat from feral swine should serve broad notice of potential threats that feral swine may pose to archaeological sites globally, making effective swine management imperative for site protection. C1 [Engeman, Richard M.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Couturier, Kathy J.] Avon Pk AF Range, Avon Pk Af Range, FL 33825 USA. [Felix, Rodney K., Jr.; Avery, Michael L.] USDA, Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL 32641 USA. RP Engeman, RM (reprint author), USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov NR 12 TC 3 Z9 3 U1 1 U2 18 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD JUN PY 2013 VL 20 IS 6 BP 4093 EP 4098 DI 10.1007/s11356-012-1367-1 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA 142EL UT WOS:000318779200057 PM 23224506 ER PT J AU Ma, JC Ibekwe, AM Yang, CH Crowley, DE AF Ma, Jincai Ibekwe, Abasiofiok M. Yang, Ching-Hong Crowley, David E. TI Influence of bacterial communities based on 454-pyrosequencing on the survival of Escherichia coli O157:H7 in soils SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE contamination; fresh produce; organic; conventional ID MANURE-AMENDED SOIL; O157-H7; DIVERSITY; BIODIVERSITY; PERSISTENCE; MANAGEMENT; STABILITY; SALINITY AB Shiga toxin-producing Escherichia coli O157:H7 has been implicated in many foodborne illnesses. In this study, survival of E.coli O157:H7 in 32 soils from California (CA) and Arizona (AZ) was investigated. Our goal was to correlate the survival time of E.coli O157:H7 in soils with 16S rRNA pyrosequencing based bacterial community composition. Kohonen self-organizing map of survival and associated soil chemical, physical and biological variables using artificial neural network analysis showed that survival of E.coli O157:H7 in soils was negatively correlated with salinity (EC), but positively correlated with total nitrogen (TN) and water soluble organic carbon (WSOC). Bacterial diversity as determined by the Shannon diversity index had no significant (P=0.635) effect on ttd, but individual bacterial phyla had different effects. The survival of E.coli O157:H7 was positively correlated with the abundances of Actinobacteria (P<0.001) and Acidobacteria (P<0.05), and negatively correlated with those of Proteobacteria and Bacteroidetes (P<0.05). Our data showed that specific groups of bacteria correlate with the persistence of E.coli O157:H7 in soils thus opening new ways to study the influence of certain bacterial phyla on persistence of this pathogen and other related pathogens in complex environments. C1 [Ma, Jincai; Ibekwe, Abasiofiok M.] ARS, USDA, US Salin Lab, Riverside, CA 92507 USA. [Yang, Ching-Hong] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53201 USA. [Ma, Jincai; Crowley, David E.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Ibekwe, AM (reprint author), ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Mark.Ibekwe@ars.usda.gov RI Crowley, David/C-1216-2014; Ma, Jincai/D-1290-2013 OI Crowley, David/0000-0002-1805-8599; Ma, Jincai/0000-0002-0792-0251 FU CSREES NIFA Agreement [2008-35201-18709]; USDA-ARS FX This research was supported by CSREES NIFA Agreement No., 2008-35201-18709 and the 206 Manure and Byproduct Utilization Project of the USDA-ARS. We thank Drs Jorge Fonseca of the University of Arizona Yuma, Mark Trent, UC-Davis, Imperial Agricultural Experiment Station, and James McCreight of USDA-ARS Salinas CA for providing soil samples for this study. We also thank Damon Baptista for technical help and Sam Henshaw for facilitating the soil sampling and transport to the laboratory. Mention of trademark or propriety products in this manuscript does not constitute a guarantee or warranty of the property by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 44 TC 11 Z9 12 U1 4 U2 63 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0168-6496 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD JUN PY 2013 VL 84 IS 3 BP 542 EP 554 DI 10.1111/1574-6941.12083 PG 13 WC Microbiology SC Microbiology GA 144IG UT WOS:000318932300009 PM 23360569 ER PT J AU Bregitzer, P Brown, RH AF Bregitzer, Phil Brown, Ryan H. TI Long-term assessment of transgene behavior in barley: Ds-mediated delivery of bar results in robust, stable, and heritable expression SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Barley; Transposition; Transgene expression; Transgene heritability ID TUMEFACIENS C58 DERIVATIVES; T-DNA; GENE-EXPRESSION; PARTICLE BOMBARDMENT; EFFICIENT METHOD; LAUNCH PADS; PLANTS; RICE; TRANSFORMATION; LINES AB The utility of transgenic plants for both experimental and practical agronomic purposes is highly dependent on stable, predictable, and heritable expression of the introduced genes. This requirement is frequently unfulfilled, and transgenes often are completely silenced. Studies of transgenic loci have shown that rearrangements of transgenes occur during the integration process, some of which are potent cues that induce silencing. Conversely, intact, single-copy transgenes produced via transposon-mediated gene delivery have shown relatively stable expression, at least in early-generation progeny. To examine the long-term expression stability of a bar expression cassette delivered via Dissociation (Ds)-mediated transposition, we examined qualitative and quantitative expression in barley (Hordeum vulgare L.) populations developed for transposon tagging. Qualitative assessments of herbicide resistance among 106 lines showed bar expression to be stable for at least five generations of advance via self-pollination. Similarly, qualitatively stable expression was observed among 31 near-isogenic lines derived from at least seven backcrosses to the cultivar Garnet. Quantitative RT-PCR measurements of bar expression were conducted for eight near-isogenic lines and their donor parents. The expression of bar was highly correlated in parent and progeny near-isogenic lines, showing high heritability of bar expression. These data demonstrate stable, predictable transgene expression following Ds-mediated delivery. C1 [Bregitzer, Phil; Brown, Ryan H.] ARS, Natl Small Grains Germplasm Res Facil, USDA, Aberdeen, ID 83210 USA. RP Bregitzer, P (reprint author), ARS, Natl Small Grains Germplasm Res Facil, USDA, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM Phil.Bregitzer@ars.usda.gov FU USDA-ARS CRIS [5366-21000-028] FX This work was funded by the USDA-ARS CRIS project 5366-21000-028. The USDA-ARS is an equal opportunity employer. NR 47 TC 5 Z9 5 U1 1 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2013 VL 49 IS 3 BP 231 EP 239 DI 10.1007/s11627-013-9507-y PG 9 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 146HH UT WOS:000319080400001 ER PT J AU Brown, RH Raboy, V Bregitzer, P AF Brown, Ryan H. Raboy, Victor Bregitzer, Phil TI Unintended consequences: high phosphinothricin acetyltransferase activity related to reduced fitness in barley SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Barley; bar; Phosphinothricin acetyltransferase; Pleiotropy ID GENETICALLY-ENGINEERED PLANTS; SELECTABLE MARKER GENES; LOW-PHYTATE MUTATIONS; AGRONOMIC PERFORMANCE; SOMACLONAL VARIATION; SCIENTISTS ANALYSIS; TRANSGENIC BARLEY; EFFICIENT METHOD; TISSUE-CULTURE; TRANSCRIPTOME AB Selectable markers used in plant transformation, such as the bar gene, have been chosen for selection efficacy and for the absence of pleiotropic effects. Recent research has suggested that expression of bar in Arabidopsis affects the transcriptome, and in at least one barley cultivar, high bar expression has been associated with severe fitness reductions. However, the observed sensitivity in barley was thought to be an isolated case associated with a specific genetic stock. In this study, fitness reductions were observed in some near-isogenic lines in the background of the cultivar Garnet when the bar locus was homozygous. The fitness of the near-isogenic lines was correlated with the levels of bar mRNA in the donor parents. Previous studies had shown heritability of bar expression in F-1 barley hybrids, so these results suggested that Garnet barley is intolerant of high phosphinothricin acetyltransferase activity. Phosphinothricin acetyltransferase activities were determined for eight near-isogenic lines that showed a range of phenotypes (apparently normal to abnormal and nonviable) when homozygous for bar. Fitness was clearly and negatively correlated with phosphinothricin acetyltransferase activity. These results suggest genetic variability for sensitivity to bar expression in barley, and the possibility of negative but non-obvious effects in apparently tolerant cultivars cannot be ruled out. Similar problems may be associated, but not yet detected, with other selectable markers. The potential for pleiotropic effects of selectable markers can be avoided by transformation strategies that enable the elimination of selectable markers. C1 [Brown, Ryan H.; Raboy, Victor; Bregitzer, Phil] ARS, Natl Small Grains Germplasm Res Facil, USDA, Aberdeen, ID 83210 USA. RP Bregitzer, P (reprint author), ARS, Natl Small Grains Germplasm Res Facil, USDA, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM Phil.Bregitzer@ars.usda.gov FU Agricultural Research Service, USDA [5366-21000-028-00, 5366-21000-025-00] FX This research was supported by the Agricultural Research Service, USDA, projects 5366-21000-028-00 and 5366-21000-025-00. The USDA-ARS is an equal opportunity employer. NR 31 TC 0 Z9 1 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2013 VL 49 IS 3 BP 240 EP 247 DI 10.1007/s11627-013-9506-z PG 8 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 146HH UT WOS:000319080400002 ER PT J AU Benyon, LS Stover, E Bowman, KD Niedz, R Shatters, RG Zale, J Belknap, W AF Benyon, Lesley S. Stover, Ed Bowman, Kim D. Niedz, Randall Shatters, Robert G., Jr. Zale, Janice Belknap, William TI GUS expression driven by constitutive and phloem-specific promoters in citrus hybrid US-802 SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Beta-glucuronidase; Genetic engineering; HLB; Huanglongbing; Transgenics ID BETA-GLUCURONIDASE; TRANSGENIC PLANTS; GENE; RICE; FUSIONS; TOBACCO; SENSE AB Transgenic solutions are being widely explored to develop huanglongbing (HLB) resistance in citrus. A critical component of a transgenic construct is the promoter, which determines tissue specificity and level of target gene expression. This study compares the characteristics of five promoters regulating the beta-glucuronidase (GUS) reporter gene in the trifoliate hybrid rootstock US-802. Two of the selected promoters direct high levels of constitutive transgene expression in other dicotyledonous plants: 2X35S, the tandem-repeat promoter of the cauliflower mosaic virus 35S gene and bul409S, a truncation of the potato polyubiquitin promoter. Because Candidatus Liberibacter, the Gram-negative bacterium associated with HLB, infects only the phloem tissue, it may be advantageous to limit transgene expression to the vascular tissue and reduce expression in the fruit. Thus, we also tested three promoters that demonstrate phloem specificity when transformed and expressed in other plants: WDV, from wheat dwarf geminivirus; AtSUC2, the sucrose-H+ symporter gene promoter from Arabidopsis; and CsSUS, the sucrose synthase promoter from citrus. Histochemical staining for GUS activity was observed throughout leaf and stem tissues for the constitutive promoters, while the three phloem-specific promoters largely showed the expected tissue-specific staining. Expression of GUS in some individual transformants with promoters CsSUS and WDV appeared leaky, with some laminar tissue staining. Relative quantification of qRT-PCR data revealed a wide range of mRNA abundance from transgenics with each of the five promoters. Fluorometry also revealed that GUS activity differed depending on the promoter used, but mRNA levels and enzyme activity were not highly correlated. C1 [Benyon, Lesley S.; Stover, Ed; Bowman, Kim D.; Niedz, Randall; Shatters, Robert G., Jr.; Zale, Janice] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Belknap, William] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Stover, E (reprint author), ARS, US Hort Res Lab, USDA, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM Lesley.Benyon@ars.usda.gov; Ed.Stover@ars.usda.gov; Kim.Bowman@ars.usda.gov; Randall.Niedz@ars.usda.gov; Robert.Shatters@ars.usda.gov; drjanicezale@gmail.com; William.Belknap@ars.usda.gov NR 35 TC 4 Z9 5 U1 2 U2 45 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2013 VL 49 IS 3 BP 255 EP 265 DI 10.1007/s11627-013-9509-9 PG 11 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 146HH UT WOS:000319080400004 ER PT J AU Reed, BM Wada, S DeNoma, J Niedz, RP AF Reed, Barbara M. Wada, Sugae DeNoma, Jeanine Niedz, Randall P. TI Improving in vitro mineral nutrition for diverse pear germplasm SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Pyrus; Micropropagation; Mineral nutrition; Growth medium; Surface response design ID TOBACCO TISSUE CULTURES; MICROPROPAGATION; INVITRO; ROOTSTOCK; GROWTH AB Mineral nutrition in the media used for growth of in vitro plants is often difficult to optimize due to complex chemical interactions of required nutrients. The response of plant tissue to standard growth media varies widely due to the genetic diversity of the plant species studied. This study was designed as the initial step in determining the optimal mineral nutrient requirements for micropropagation of shoot tips from a collection of genetically diverse pear germplasm. Five mineral nutrient factors were defined from Murashige and Skoog salts: NH4NO3, KNO3, mesos (CaCl2I double dagger 2H(2)0-KH2PO4-MgSO4), micronutrients (B, Cu, Co, I, Mn, Mo, and Zn), and Fe-EDTA. Each factor was varied over a range of concentrations. Treatment combinations were selected using response surface methods. Five pears in three species (Pyrus communis 'Horner 51,' 'Old Home x Farmingdale 87,' 'Winter Nelis,' Pyrus dimorphophylla, and Pyrus ussuriensis 'Hang Pa Li') were grown on each treatment combination, responses were measured, and each response was analyzed by analysis of variance. The analyses resulted in the identification of the following factors with the single largest effects on plant response: shoot quality (mesos), leaf spotting/necrosis (mesos), leaf size (mesos), leaf color (mesos, NH4NO3, and KNO3), shoot number (NH4NO3 and Fe), nodes (NH4NO3 and KNO3), and shoot length (mesos and Fe). Factors with the largest effects (mesos and Fe) were similar among the genotypes. This approach was very successful for defining the appropriate types and concentrations of mineral nutrients for micropropagation of diverse pear genotypes. C1 [Reed, Barbara M.; DeNoma, Jeanine] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Niedz, Randall P.] ARS, Hort & Breeding Unit, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Reed, BM (reprint author), ARS, USDA, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97333 USA. EM Barbara.Reed@ars.usda.gov OI Reed, Barbara/0000-0003-0079-8473 FU Oregon Association of Nurseries; Oregon Department of Agriculture; United States Department of Agriculture-Agricultural Research Service CRIS [5358-21000-0-38-00D] FX We thank NCGR lab personnel for assistance with collection of the data for this study. This project was funded by a grant from the Oregon Association of Nurseries and the Oregon Department of Agriculture and by United States Department of Agriculture-Agricultural Research Service CRIS project 5358-21000-0-38-00D. NR 26 TC 21 Z9 21 U1 1 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD JUN PY 2013 VL 49 IS 3 BP 343 EP 355 DI 10.1007/s11627-013-9504-1 PG 13 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA 146HH UT WOS:000319080400014 ER PT J AU Mann, RS Rouseff, RL Smoot, J Rao, N Meyer, WL Lapointe, SL Robbins, PS Cha, D Linn, CE Webster, FX Tiwari, S Stelinski, LL AF Mann, Rajinder S. Rouseff, Russell L. Smoot, Jack Rao, Nandikeswara Meyer, Wendy L. Lapointe, Stephen L. Robbins, Paul S. Cha, Dong Linn, Charles E. Webster, Francis X. Tiwari, Siddharth Stelinski, Lukasz L. TI Chemical and behavioral analysis of the cuticular hydrocarbons from Asian citrus psyllid, Diaphorina citri SO INSECT SCIENCE LA English DT Article DE citrus; cuticular hydrocarbon; dodecanoic acid; Huanglongbing; semiochemical ID KUWAYAMA HEMIPTERA PSYLLIDAE; INFESTED PEAR SHOOTS; FEMALE SEX-PHEROMONE; TARNISHED PLANT BUG; AGGREGATION PHEROMONE; CACOPSYLLA-PYRICOLA; INSECT HYDROCARBONS; LYGUS-RUGULIPENNIS; FIELD; ATTRACTION AB Huanglongbing (HLB) is the most destructive disease of citrus worldwide. The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the vector of the phloem-inhabiting bacterium, Candidatus Liberibacter asiaticus, which is presumed to cause HLB in Florida citrus. Laboratory and field studies were conducted to examine the behavioral responses of male and female D. citri to their cuticular extracts. In olfactometer assays, more male D. citri were attracted to one, five, or 10 female cuticular extract equivalent units than blank controls. The results were confirmed in field studies in which clear or yellow traps baited with 10 female cuticular extract equivalent units attracted proportionately more males than clear traps baited with male cuticular extract or unbaited traps. Analyses of cuticular constituents of male and female D. citri revealed differences between the sexes in chemical composition of their cuticular extracts. Laboratory bioassays with synthetic chemicals identified from cuticular extracts indicated that dodecanoic acid attracted more males than clean air. Traps baited with dodecanoic acid did not increase total catch of D. citri as compared with blank traps at the dosages tested; however, the sex ratio of psyllid catch was male biased on traps baited with the highest lure loading dosage tested (10.0 mg). C1 [Mann, Rajinder S.; Meyer, Wendy L.; Tiwari, Siddharth; Stelinski, Lukasz L.] Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Rouseff, Russell L.; Smoot, Jack] Univ Florida, Flavor & Volatile Anal Grp, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Rao, Nandikeswara] Natl Res Ctr Citrus, Nagpur, Maharashtra, India. [Lapointe, Stephen L.; Robbins, Paul S.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Cha, Dong; Linn, Charles E.] Cornell Univ, Dept Entomol, NYS Agr Expt Stn, Geneva, NY 14456 USA. [Webster, Francis X.] SUNY Coll Environm Sci & Forestry, Dept Chem, Syracuse, NY 13210 USA. RP Stelinski, LL (reprint author), Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. EM stelinski@ufl.edu FU Citrus Research and Development Foundation, Florida, USA FX We thank Angel Hoyte and Michael Flores for help with insect maintenance and bioassays. This work was supported by a grant from the Citrus Research and Development Foundation, Florida, USA. NR 59 TC 7 Z9 7 U1 4 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 J9 INSECT SCI JI Insect Sci. PD JUN PY 2013 VL 20 IS 3 SI SI BP 367 EP 378 DI 10.1111/j.1744-7917.2012.01541.x PG 12 WC Entomology SC Entomology GA 144OP UT WOS:000318949600010 PM 23955888 ER PT J AU Evans, GA Kilkenny, FF Galloway, LF AF Evans, Gregory A. Kilkenny, Francis F. Galloway, Laura F. TI EVOLUTION OF COMPETITIVE ABILITY WITHIN LONICERA JAPONICA'S INVADED RANGE SO INTERNATIONAL JOURNAL OF PLANT SCIENCES LA English DT Article DE intraspecific competition; invasive species; vine; population differentiation; Japanese honeysuckle ID POPPIES ESCHSCHOLZIA-CALIFORNICA; LOCAL ADAPTATION; RAPID EVOLUTION; INVASIVE PLANT; DIVERSITY; LIANAS; LIMITS; PLASTICITY; DISPERSAL; ECOLOGY AB Factors influencing invasive taxa may change during the course of an invasion. For example, intraspecific competition is predicted to be more important in areas with older stands of dense monospecific invaders than at the margins of an invaded range. We evaluated evolution in response to predicted changes in competition by comparing the intraspecific competitive ability of North American populations of Japanese honeysuckle (Lonicera japonica) from areas established 100-150 yr ago, which make up the core of the invaded range, to populations from the northern range margin, established in the past 50 yr. Plants from populations in the core and margin regions of the invaded range were grown in two common garden studies. The first had three levels of intraspecific competition, and vines differed in their access to a lattice for climbing. The second asked whether plants from older core or newer margin populations were better competitors. Intraspecific competition decreased the size of plants from both core and margin regions. Contrary to predictions, there was no increase in climbing shoot frequency or size in plants from denser core populations, although these did increase in the presence of a lattice structure. Also contrary to predictions, plants from the margin of the invaded range were larger and stronger competitors than those from the core, regardless of competition level. Enhanced intraspecific competitive ability of plants from low-density margin populations is likely driven by selection for more rapid growth rate in the north, rather than direct selection on competitive ability. Results counter to prediction highlight the importance of considering the broader selective context to understand evolution. Furthermore, this differentiation across the invaded range is likely to enhance L. japonica's invasive potential. C1 [Evans, Gregory A.; Kilkenny, Francis F.; Galloway, Laura F.] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA. RP Kilkenny, FF (reprint author), USDA, Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM ffkilkenny@fs.fed.us NR 46 TC 3 Z9 3 U1 1 U2 75 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 1058-5893 J9 INT J PLANT SCI JI Int. J. Plant Sci. PD JUN PY 2013 VL 174 IS 5 BP 740 EP 748 DI 10.1086/669928 PG 9 WC Plant Sciences SC Plant Sciences GA 145YN UT WOS:000319056100002 ER PT J AU Eicher, SD Lay, DC Arthington, JD Schutz, MM AF Eicher, S. D. Lay, D. C., Jr. Arthington, J. D. Schutz, M. M. TI Effects of rubber flooring during the first 2 lactations on production, locomotion, hoof health, immune functions, and stress SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE cow comfort; dairy cow; flooring; immunity ID DAIRY-COWS; CLAW HEALTH; TRANSITION PERIOD; MONONUCLEAR-CELLS; SERUM HAPTOGLOBIN; FREESTALL BARNS; MILK-PRODUCTION; LAMENESS; CATTLE; PAIN AB Some housing systems on dairy farms can result in long-term chronic pain. The effects of acute pain on immunity have been explored, but chronic pain's influence on immune responses is still poorly understood. Therefore, the objective of this research was to determine chronic effects of flooring on immune responses and production in freestall housing for dairy cows. Thirty heifers were studied from before calving as first-calf heifers until d 180 of their second lactation. Treatments were rubber (Kraiburg; Agromatic Inc., Fond du Lac, WI) flooring or concrete with diamond grooves in a freestall barn, each in 2 quadrants of the barn. Heifers entered the treatments after calving, so the system was dynamic and each cow was considered an experimental unit. At the end of the first lactation, cows were housed in a bedded pack barn with pasture access until calving was imminent. At that time, they returned to their assigned treatment, but not necessarily into the same quadrant. Production, reproduction, cortisol, acute-phase proteins, and health data were recorded throughout lactation 1, locomotion was scored weekly, and hoof scoring and care was conducted on d 60 and 180 of lactations 1 and 2, and quantitative real-time-PCR of blood leukocytes was analyzed in mid lactation of lactation 1. Mature-equivalent milk fat, milk protein, and protein percentages during the first lactation were greater for cows on the rubber flooring. Hoof and leg therapy treatments per cow were fewer for rubber floor-housed cows. Locomotion scores were less for cows housed on rubber during the second lactation. White blood cell counts were less for cows housed on rubber, and caused by greater lymphocyte counts for cows housed on concrete. The possibility of chronic inflammation was substantiated by less IL-1 beta and more IL-1 receptor antagonists for cows housed on rubber at d 150 in the second lactation. Cortisol and acute-phase proteins did not differ between the treatments. Interferon-gamma, IL-12, the modulator of tissue reconstruction (B-cell-transforming growth factor 1), and pain-modulating neurokinin (tachykinin 1) were not different at d 105. These data show indicators of chronic inflammation for cows housed on the concrete flooring compared with those housed on rubber. Implications for the use of rubber flooring in freestall barns are broader than just lameness and may affect many aspects of cow physiology and production. C1 [Eicher, S. D.; Lay, D. C., Jr.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Arthington, J. D.] Univ Florida, Range Cattle Res & Educ Ctr, Gainesville, FL 32601 USA. [Schutz, M. M.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. RP Eicher, SD (reprint author), Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM Susan.Eicher@ars.usda.gov NR 42 TC 7 Z9 7 U1 5 U2 53 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD JUN PY 2013 VL 96 IS 6 BP 3639 EP 3651 DI 10.3168/jds.2012-6049 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 145OX UT WOS:000319027600026 PM 23587383 ER PT J AU Jeong, J Kannan, N Arnold, JG Glick, R Gosselink, L Srinivasan, R Barrett, ME AF Jeong, Jaehak Kannan, Narayanan Arnold, Jeff G. Glick, Roger Gosselink, Leila Srinivasan, Raghavan Barrett, Michael E. TI Modeling Sedimentation-Filtration Basins for Urban Watersheds Using Soil and Water Assessment Tool SO JOURNAL OF ENVIRONMENTAL ENGINEERING-ASCE LA English DT Article DE Best management practice; Storm-water management; Runoff; Sediment; Sand filters ID URBANIZATION; INFILTRATION; RUNOFF; MEDIA AB Sedimentation-filtration (SedFil) basins are one of the storm-water best management practices (BMPs) that are intended to mitigate water quality problems in urban creeks and rivers. A new physically based model of variably saturated flows was developed for simulating flow and sediment in SedFils within the Soil and Water Assessment Tool (SWAT). The integrated SWAT-SedFil model allows for simulation of unsaturated flow in the filtration basin during small storms and fully saturated flow. Unsaturated flow was modeled using a modified Green and Ampt equation, and saturated flow was simulated with Darcy's Law. Unsaturated flow comprises only a small fraction of large storm events; however, many regular storms are small and may not generate sufficient runoff to create a saturated flow in the filtration basin. Therefore, the combined unsaturated/saturated flow approach for modeling SedFils improved the accuracy of the model, especially in long-term evaluations. The model performs well with respect to estimating storm-water and sediment at the inlet and outlet of a SedFil. (C) 2013 American Society of Civil Engineers. C1 [Jeong, Jaehak; Kannan, Narayanan] Texas AgriLife Res, Blackland Res Ctr, Temple, TX 76502 USA. [Arnold, Jeff G.] ARS, USDA, Temple, TX 76502 USA. [Glick, Roger; Gosselink, Leila] City Austin, Watershed Protect Dept, Austin, TX 78767 USA. [Srinivasan, Raghavan] Texas A&M Univ, Spatial Sci Lab, College Stn, TX 77845 USA. [Barrett, Michael E.] Univ Texas Austin, Ctr Res Water Resources, Austin, TX 78712 USA. RP Jeong, J (reprint author), Texas AgriLife Res, Blackland Res Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. EM jjeong@brc.tamus.edu RI Srinivasan, R/D-3937-2009 FU Department of Watershed Protection, City of Austin, Texas FX The writers thank the Department of Watershed Protection, City of Austin, Texas, for providing funding for the study. NR 28 TC 5 Z9 5 U1 1 U2 38 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9372 J9 J ENVIRON ENG-ASCE JI J. Environ. Eng.-ASCE PD JUN PY 2013 VL 139 IS 6 BP 838 EP 848 DI 10.1061/(ASCE)EE.1943-7870.0000691 PG 11 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 142LT UT WOS:000318799600009 ER PT J AU Bruins, MJ Dane, AD Strassburg, K Vreeken, RJ Newman, JW Salem, N Tyburczy, C Brenna, JT AF Bruins, Maaike J. Dane, Adrie D. Strassburg, Katrin Vreeken, Rob J. Newman, John W. Salem, Norman, Jr. Tyburczy, Cynthia Brenna, J. Thomas TI Plasma oxylipin profiling identifies polyunsaturated vicinal diols as responsive to arachidonic acid and docosahexaenoic acid intake in growing piglets SO JOURNAL OF LIPID RESEARCH LA English DT Article DE lipidomics; metabolomics; eicosanoid; prostaglandin; omega-3; omega-6 polyunsaturated fatty acid ID RANDOMIZED CONTROLLED-TRIAL; FATTY-ACIDS; TERM INFANTS; EPOXYEICOSATRIENOIC ACIDS; CYTOCHROME-P450 ENZYMES; COMPARTMENTAL ANALYSES; DOMESTIC PIGLETS; LIPID MEDIATORS; KNOCKOUT MICE; PROSTAGLANDINS AB The dose-responsiveness of plasma oxylipins to incremental dietary intake of arachidonic acid (20:4n-6; ARA) and docosahexaenoic acid (22:6n-3; DHA) was determined in piglets. Piglets randomly received one of six formulas (n = 8 per group) from days 3 to 27 postnatally. Diets contained incremental ARA or incremental DHA levels as follows (% fatty acid, ARA/DHA): (A1) 0.1/1.0; (A2) 0.53/1.0; (A3-D3) 0.69/1.0; (A4) 1.1/1.0; (D1) 0.66/0.33; and (D2) 0.67/0.62, resulting in incremental intake (g/kg BW/day) of ARA: 0.07 +/- 0.01, 0.43 +/- 0.03, 0.55 +/- 0.03, and 0.82 +/- 0.05 at constant DHA intake (0.82 +/- 0.05), or incremental intake of DHA: 0.27 +/- 0.02, 0.49 +/- 0.03, and 0.81 +/- 0.05 at constant ARA intake (0.54 +/- 0.04). Plasma oxylipin concentrations and free plasma PUFA levels were determined at day 28 using LC-MS/MS. Incremental dietary ARA intake dose-dependently increased plasma ARA levels. In parallel, ARA intake dose-dependently increased ARA-derived diols 5,6- and 14,15-dihydroxyeicosatrienoic acid (DiHETrE) and linoleic acid-derived 12,13-dihydroxyoctadecenoic acid (DiHOME), downstream metabolites of cytochrome P450 expoxygenase (CYP). The ARA epoxide products from CYP are important in vascular homeostatic maintenance. Incremental DHA intake increased plasma DHA and most markedly raised the eicosapentaenoic acid (EPA) metabolite 17,18-dihydroxyeicosatetraenoic acid (DiHETE) and the DHA metabolite 19,20-dihydroxydocosapentaenoic acid (DiHDPE). In conclusion, increasing ARA and DHA intake dose-dependently influenced endogenous n-6 and n-3 oxylipin plasma concentrations in growing piglets, although the biological relevance of these findings remains to be determined. C1 [Bruins, Maaike J.] DSM Biotechnol Ctr, Delft, Netherlands. [Dane, Adrie D.; Strassburg, Katrin; Vreeken, Rob J.] Leiden Univ, NMC, LACDR, Leiden, Netherlands. [Newman, John W.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Salem, Norman, Jr.] DSM Nutr Lipids, Columbia, MD USA. [Tyburczy, Cynthia; Brenna, J. Thomas] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. RP Brenna, JT (reprint author), Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. EM jtb4@cornell.edu FU DSM Biotechnology Center (Delft, The Netherlands); DSM Biotechnology Center (Columbia, MD); US Department of Agriculture (USDA); Agricultural Research Service (ARS); CRIS project [5306-51530-019-00D]; the Netherlands Metabolomics Centre (NMC), which is a part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research FX This work was supported by DSM Biotechnology Center (Delft, The Netherlands, and Columbia, MD) (C.T. and J.T.B.) and by US Department of Agriculture (USDA), Agricultural Research Service (ARS), CRIS project 5306-51530-019-00D (J.W.N.). The plasma and statistical analyses were (co)financed by the Netherlands Metabolomics Centre (NMC), which is a part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (R.J.V. and K.S.). NR 69 TC 12 Z9 13 U1 0 U2 30 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0022-2275 J9 J LIPID RES JI J. Lipid Res. PD JUN PY 2013 VL 54 IS 6 BP 1598 EP 1607 DI 10.1194/jlr.M034918 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 143IA UT WOS:000318859400009 PM 23543770 ER PT J AU Tilt, JH Cerveny, L AF Tilt, Jenna H. Cerveny, Lee TI Master-planned in exurbia: Examining the drivers and impacts of master-planned communities at the urban fringe SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Population growth; Land use change; Housing preference; Natural amenities; Community cohesion; Community design ID UNITED-STATES; LAND-USE; CONSERVATION VALUE; GATED COMMUNITIES; BUILT ENVIRONMENT; PROTECTED AREAS; HOUSING GROWTH; SENSE; NEIGHBORHOODS; ESTATE AB Smart growth strategies of infill and compact growth in existing suburban cities will most likely not be sufficient to absorb a new US household growth in the future. To meet housing demands and preferences, master-planned communities will continue to be built in outlying exurban areas. However, little is known about the impacts these communities may have on the surrounding physical, built and social environment in the exurban landscape. In this paper, we provide a review of the literature of what is known about the drivers behind the development of master-planned communities and the physical, built and social impacts of these developments on the surrounding exurban landscape. We then provide a case study of an exurban area outside of Seattle, Washington containing a large master-planned community. Through focus group interviews, we explore residential motivations to move to the area, and the benefits and challenges of living in an exurban landscape with a newly built master-planned community. Using qualitative data analyses, we find that residents are drawn to this exurban area for the abundance of natural amenities and outdoor recreation opportunities. However, the new master-planned community development presents many benefits and challenges for those living in the area; particularly the residential perceptions of impacts that the development has had on the surrounding natural and built environment. These real and perceived impacts of the master-planned community development has compounded and magnified the impacts to the social environment throughout the entire exurban community. Implications for planners and suggestions for future research are given. (C) 2013 Elsevier B.V. All rights reserved. C1 [Tilt, Jenna H.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Cerveny, Lee] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Tilt, JH (reprint author), Oregon State Univ, Coll Earth Ocean & Atmospher Sci, 104 CEOAS Admin Bldg, Corvallis, OR 97331 USA. EM tiltj@onid.orst.edu; lcerveny@fs.fed.us FU Pacific Northwest Research Station, Forest Service, U.S. Department of Agriculture [10-JV-11260489-046] FX This research was supported in part by funds provided by the Pacific Northwest Research Station, Forest Service, U.S. Department of Agriculture, under Agreement No. 10-JV-11260489-046. We thank the focus group participants for taking time to share their thoughts and ideas with us. We also thank the anonymous reviewers for their insightful comments and suggestions. NR 78 TC 4 Z9 4 U1 2 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD JUN PY 2013 VL 114 BP 102 EP 112 DI 10.1016/j.landurbplan.2013.03.003 PG 11 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 142ZW UT WOS:000318837100010 ER PT J AU Miyagi, A Uchimiya, M Kawai-Yamada, M Uchimiya, H AF Miyagi, Atsuko Uchimiya, Minori Kawai-Yamada, Maki Uchimiya, Hirofumi TI An antagonist treatment in combination with tracer experiments revealed isocitrate pathway dominant to oxalate biosynthesis in Rumex obtusifolius L. SO METABOLOMICS LA English DT Article DE Rumex obtusifolius L.; Capillary electrophoresis-mass spectrometry; Oxalate; Citrate; Itaconate; Isocitrate lyase ID L-ASCORBIC-ACID; OXALIC-ACID; CALCIUM-OXALATE; LEAF SHEATHS; LYASE; PLANTS; EXPRESSION; CRISPUS; OXIDASE; DOCK AB Oxalate accumulates in leaves of certain plants such as Rumex species (Polygonaceae). Oxalate plays important roles in defense to predator, detoxification of metallic ions, and in hydrogen peroxide formation upon wounding/senescence. However, biosynthetic pathways of soluble oxalate are largely unknown. In the present study we analysed Rumex obtusifolius L. treated with itaconate (an antagonist to isocitrate). Comprehensive metabolome analysis using capillary electrophoresis-mass spectrometry showed that oxalate content of "new leaves" was notably down-regulated by itaconate, as opposed to the accumulation of citrate. The (CO2)-C-13 feeding experiment revealed that oxalate accumulation in new leaves was affected by citrate translocation from stems. The results suggested that excess oxalate in new leaves of R. obtusifolius was synthesized primarily via the isocitrate pathway utilizing citrate delivered from stems. C1 [Miyagi, Atsuko; Kawai-Yamada, Maki; Uchimiya, Hirofumi] Saitama Univ, Inst Environm Sci & Technol, Sakura Ku, Saitama 3388570, Japan. [Uchimiya, Minori] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Kawai-Yamada, Maki] Saitama Univ, Grad Sch Sci & Engn, Sakura Ku, Saitama 3388570, Japan. RP Uchimiya, H (reprint author), Saitama Univ, Inst Environm Sci & Technol, Sakura Ku, 225 Shimo Okubo, Saitama 3388570, Japan. EM cuchimiya@hotmail.com RI Miyagi, Atsuko/N-8621-2015 FU Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan; MEXT, Japan FX This research was supported by a grant from the Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry (BRAIN), Japan, and a grand from MEXT, Japan. We thank Dr. Ko Noguchi (The University of Tokyo), Dr. Masatoshi Yamaguchi (Saitama University) and Dr. Kentaro Takahara (The University of Tokyo) for helpful advice. NR 28 TC 7 Z9 7 U1 2 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 J9 METABOLOMICS JI Metabolomics PD JUN PY 2013 VL 9 IS 3 BP 590 EP 598 DI 10.1007/s11306-012-0486-5 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 143YL UT WOS:000318905100007 ER PT J AU Pliego, C Nowara, D Bonciani, G Gheorghe, DM Xu, R Surana, P Whigham, E Nettleton, D Bogdanove, AJ Wise, RP Schweizer, P Bindschedler, LV Spanu, PD AF Pliego, Clara Nowara, Daniela Bonciani, Giulia Gheorghe, Dana M. Xu, Ruo Surana, Priyanka Whigham, Ehren Nettleton, Dan Bogdanove, Adam J. Wise, Roger P. Schweizer, Patrick Bindschedler, Laurence V. Spanu, Pietro D. TI Host-Induced Gene Silencing in Barley Powdery Mildew Reveals a Class of Ribonuclease-Like Effectors SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID BLUMERIA-GRAMINIS; ASPERGILLUS-FUMIGATUS; EPIDERMAL-CELLS; PROTEINS; GENOME; RESISTANCE; EXPRESSION; VIRULENCE; FUNGI; DETERMINANTS AB Obligate biotrophic pathogens of plants must circumvent or counteract defenses to guarantee accommodation inside the host. To do so, they secrete a variety of effectors that regulate host immunity and facilitate the establishment of pathogen feeding structures called haustoria. The barley powdery mildew fungus Blumeria graminis f. sp. hordei produces a large number of proteins predicted to be secreted from haustoria. Fifty of these Blumeria effector candidates (BEC) were screened by host-induced gene silencing (HIGS), and eight were identified that contribute to infection. One shows similarity to beta-1,3 glucosyltransferases, one to metallo-proteases, and two to microbial secreted ribonucleases; the remainder have no similarity to proteins of known function. Transcript abundance of all eight BEC increases dramatically in the early stages of infection and establishment of haustoria, consistent with a role in that process. Complementation analysis using silencing-insensitive synthetic cDNAs demonstrated that the ribonuclease-like BEC 1011 and 1054 are bona fide effectors that function within the plant cell. BEC1011 specifically interferes with pathogen-induced host cell death. Both are part of a gene superfamily unique to the powdery mildew fungi. Structural modeling was consistent, with BEC1054 adopting a ribonuclease-like fold, a scaffold not previously associated with effector function. C1 [Pliego, Clara; Bonciani, Giulia; Gheorghe, Dana M.; Spanu, Pietro D.] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, London SW7 2AZ, England. [Nowara, Daniela; Schweizer, Patrick] Leibniz Inst Plant Genet & Crop Plant Res, D-06466 Gatersleben, Germany. [Xu, Ruo] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Surana, Priyanka] Iowa State Univ, Bioinformat & Computat Biol Grad Program, Ames, IA 50011 USA. [Surana, Priyanka; Whigham, Ehren; Nettleton, Dan; Bogdanove, Adam J.; Wise, Roger P.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Surana, Priyanka; Whigham, Ehren; Nettleton, Dan; Bogdanove, Adam J.; Wise, Roger P.] Iowa State Univ, Ctr Plant Responses Environm Stresses, Ames, IA 50011 USA. [Wise, Roger P.] Iowa State Univ, Corn Insects & Crop Genet Res Unit, USDA, ARS, Ames, IA 50011 USA. [Bindschedler, Laurence V.] Univ Reading, Dept Chem, Reading RG6 6AS, Berks, England. RP Spanu, PD (reprint author), Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, South Kensington Campus, London SW7 2AZ, England. EM p.spanu@imperial.ac.uk OI Spanu, Pietro/0000-0001-8928-6049 FU Biotechnology and Biological Sciences Research Council [BB/H001646/1]; European Union; United States National Science Foundation (Plant Genome) [09-22746] FX We thank G Fuerst (Wise Lab) for excellent technical assistance throughout the project. This research was supported by the Biotechnology and Biological Sciences Research Council (grant BB/H001646/1), the European Union (Marie Curie Fellowship to C. Pliego), and the United States National Science Foundation (Plant Genome grant 09-22746). NR 43 TC 43 Z9 45 U1 7 U2 90 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JUN PY 2013 VL 26 IS 6 BP 633 EP 642 DI 10.1094/MPMI-01-13-0005-R PG 10 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA 144SI UT WOS:000318960300004 PM 23441578 ER PT J AU Karlen, DL Kovar, JL Cambardella, CA Colvin, TS AF Karlen, Douglas L. Kovar, John L. Cambardella, Cynthia A. Colvin, Thomas S. TI Thirty-year tillage effects on crop yield and soil fertility indicators SO SOIL & TILLAGE RESEARCH LA English DT Article DE Conservation agriculture; Maize; Soybean; Soil-test P and K; Plant nutrition; Tillage system economics ID LONG-TERM; ROTATION; QUALITY; CORN; IOWA AB Long-term studies are crucial for quantifying tillage effects on productivity and soil fertility. Moldboard plow, chisel plow, spring disk, ridge-tillage, and no-tillage systems were evaluated after 32 years of a corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] rotation and 27 years of continuous corn in central Iowa, U.S.A. Productivity was quantified using yield, while soil fertility status was evaluated by collecting four, 5-cm diameter soil cores to a depth of 0.9 m from each treatment, dividing them into four depth increments (0-15-, 15-30-, 30-60-, and 60-90-cm), analyzing them for bulk density (BD), NO3-N, NH4-N, electrical conductivity (EC), pH, Mehlich-3 extractable P, K, Ca, Mg and DTPA-extractable Cu, Fe, Mn, and Zn, and interpreting the data using Iowa State University (ISU) guidelines. Production costs for each tillage and cropping system were also computed. To account for genetic and agronomic changes during the 30-year study period, yields were examined for establishment, maintenance, and intensification/recovery phases. Rotated corn yield averaged 8.6, 8.8, and 11.6 Mg ha(-1) and soybean yield averaged 2.7, 3.2, and 3.4 Mg ha(-1), respectively, for each of the phases. Continuous corn from 1988 to 2006 averaged 7.5 and 10.1 Mg ha(-1) for the maintenance and intensification/recovery phases, respectively. Fixed plus variable machinery costs for corn ranged from 233 to 354 USD ha(-1), while for soybean they ranged from 194 to 280 USD ha(-1). Net returns to land, labor and management ranged from 233 to 269, 560 to 620, and 437 to 483 USD ha(-1) for continuous corn, rotated corn, and rotated soybean, respectively. Based on 9-year (2003-2011) average grain prices and yields from this study, the corn-soybean rotation was twice as profitable as continuous corn. Soil-test P and K measurements, as well as calculated P and K removal, suggest that nutrient mining occurred during the course of this study. The soil-test data also indicate that further studies are needed regarding plant availability of subsoil K and its impact on fertilizer recommendations. Overall, we conclude that with good nutrient management and crop rotation, yield and soil fertility differences between no-tillage and more intensive tillage systems can be minimized and that no-till production can be profitable on glacial till derived soils. C1 [Karlen, Douglas L.; Kovar, John L.; Cambardella, Cynthia A.] ARS, USDA, Natl Lab Agr & Environm NLAE, Ames, IA 50011 USA. [Colvin, Thomas S.] ARS, USDA, Cambridge, IA 50046 USA. RP Karlen, DL (reprint author), ARS, USDA, Natl Lab Agr & Environm NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. EM Doug.Karlen@ars.usda.gov; John.Kovar@ars.usda.gov; Cindy.Cambardella@ars.usda.gov; oxfrdfrm@huxcomm.net NR 27 TC 26 Z9 28 U1 7 U2 102 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 J9 SOIL TILL RES JI Soil Tillage Res. PD JUN PY 2013 VL 130 BP 24 EP 41 DI 10.1016/j.still.2013.02.003 PG 18 WC Soil Science SC Agriculture GA 143SS UT WOS:000318889100005 ER PT J AU Kumar, M Marks, D Dozier, J Reba, M Winstral, A AF Kumar, Mukesh Marks, Danny Dozier, Jeff Reba, Michele Winstral, Adam TI Evaluation of distributed hydrologic impacts of temperature-index and energy-based snow models SO ADVANCES IN WATER RESOURCES LA English DT Article DE Snow; Energy-balance; Temperature-index; Hydrologic model ID SEMIARID MOUNTAIN CATCHMENT; TERRAIN-BASED PARAMETERS; SOLAR-RADIATION; UNITED-STATES; BALANCE MODELS; BOREAL FOREST; SIERRA-NEVADA; ALPINE REGION; WATER-BALANCE; TIME-SERIES AB Two commonly used strategies in modeling snowmelt are the energy balance and temperature-index methods. Here we evaluate the distributed hydrologic impacts of these two different snowmelt modeling strategies, each in conjunction with a physics-based hydrologic model (PIHM). Results illustrate that both the Isnobal energy-balance and calibrated temperature-index methods adequately reproduce snow depletion at the observation site. However, the models exhibit marked differences in the distribution of snowmelt. When combined with PIHM, both models capture streamflow reasonably during calibration year (WY06), but Isnobal model gives better streamflow results in the validation year (WY07). The uncalibrated temperature-index model predicts streamflow poorly in both years. Differences between distributed snowmelt, as predicted by Isnobal and calibrated temperature-index method, and its consequent effect on predicted hydrologic states suggest the need to carefully calibrate temperature-index models in both time and space. Combined physics-based snow and hydrologic models provide the best accuracy, while a temperature-index model using coefficients from the literature the poorest. (c) 2013 Elsevier Ltd. All rights reserved. C1 [Kumar, Mukesh] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Marks, Danny; Winstral, Adam] ARS, USDA, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Dozier, Jeff] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. [Reba, Michele] ARS, USDA, Natl Sedimentat Lab, Jonesboro, AR 72401 USA. RP Kumar, M (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM mukesh.kumar@duke.edu; ars.danny@gmail.com; dozier@bren.ucsb.edu; michele.reba@ars.usda.gov; adam.winstral@ars.usda.gov RI Dozier, Jeff/B-7364-2009 OI Dozier, Jeff/0000-0001-8542-431X FU Duke University; NASA [NNX11AK35A]; NSF [CBET-0838607]; NSF-CBET [0854553]; USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West [5362-13610-008-00D]; USDA-NRCS Water and Climate Center-Portland, Oregon [5362-13610-008-03R]; USDA-ARS Headquarters Postdoctoral Research Associate Program [0101-88888-016-00D]; USDA-NRCS Conservation Effects Assessment Project [5352-13610-009-14R]; USDA-ARS CRIS Preserving water quality and availability for agriculture in the Lower Mississippi River Basin [7408-13000-024-00D] FX The data and analysis presented in this paper were funded in part by Duke University startup grant, NASA NNX11AK35A, NSF CBET-0838607, NSF-CBET (0854553), USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West (5362-13610-008-00D), USDA-NRCS Water and Climate Center-Portland, Oregon (5362-13610-008-03R), USDA-ARS Headquarters Postdoctoral Research Associate Program-Class of 2009 (0101-88888-016-00D), USDA-NRCS Conservation Effects Assessment Project (5352-13610-009-14R), USDA-ARS CRIS Preserving water quality and availability for agriculture in the Lower Mississippi River Basin (7408-13000-024-00D). Any reference to specific equipment types or manufacturers is for information purposes and does not represent a product endorsement or recommendation. USDA is an equal opportunity provider and employer. NR 59 TC 35 Z9 35 U1 1 U2 31 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 J9 ADV WATER RESOUR JI Adv. Water Resour. PD JUN PY 2013 VL 56 BP 77 EP 89 DI 10.1016/j.advwatres.2013.03.006 PG 13 WC Water Resources SC Water Resources GA 139SY UT WOS:000318605900007 ER PT J AU Sneeringer, S AF Sneeringer, Stacy TI Differences between Livestock and Crop Producers' Participation in Nutrient Trading SO APPLIED ECONOMIC PERSPECTIVES AND POLICY LA English DT Article DE Chesapeake Bay; Concentrated Animal Feeding Operation (CAFO); Livestock; Nutrient trading; Regulation; Q01; Q50 ID ANIMAL FEEDING OPERATIONS AB Descriptions of agricultural producers participation in nutrient trading schemes generally refer to crop producers reducing nutrient run-off from fields to generate credits. However, livestock producers may participate differently than crop producers because some are regulated as Concentrated Animal Feeding Operations (CAFOs), and because agricultural producers generating manure face different costs of reducing nutrient application (and therefore generating credits) from those only using fertilizer. These differences may affect nutrient reduction credit prices and the supply of agriculturally supplied credits, thereby raising the costs to non-agricultural operations of meeting water quality goals. We examine how CAFO laws interact with nutrient trading requirements. We model trading participation by farm type and then simulate trading participation by applying the model to 2007 Census of Agriculture data. C1 Econ Res Serv, USDA, Washington, DC USA. RP Sneeringer, S (reprint author), Econ Res Serv, USDA, Washington, DC USA. EM ssneeringer@ers.usda.gov NR 28 TC 1 Z9 1 U1 0 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 2040-5790 J9 APPL ECON PERSPECT P JI Appl. Econ. Perspect. Policy PD JUN PY 2013 VL 35 IS 2 BP 296 EP 321 DI 10.1093/aepp/ppt010 PG 26 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 138YP UT WOS:000318548300006 ER PT J AU Rawles, SD Fuller, SA Beck, BH Gaylord, TG Barrows, FT McEntire, ME AF Rawles, Steven D. Fuller, S. Adam Beck, Benjamin H. Gaylord, T. Gibson Barrows, Frederic T. McEntire, Matthew E. TI Lysine optimization of a commercial fishmeal-free diet for hybrid striped bass (Morone chrysops x M. saxatilis) SO AQUACULTURE LA English DT Article DE Hybrid striped bass; Ideal protein; Lysine optimization; Fishmeal-free diet ID SALMON SALMO-SALAR; TROUT ONCORHYNCHUS-MYKISS; AMINO-ACID-REQUIREMENTS; BY-PRODUCT MEAL; ATLANTIC SALMON; RAINBOW-TROUT; SOYBEAN-MEAL; NUTRITIONAL-REQUIREMENTS; THREONINE REQUIREMENT; GROWING-PIGS AB Substitution of fishmeal with alternate proteins in aquafeeds often results in dietary imbalances of first-limiting essential amino acids (EAA) and poorer fish performance. This growth trial was undertaken to test the hypothesis that ideal protein theory accurately predicts first-limiting amino acids and optimum lysine level for a fishmeal-free, commercial-grade diet formulated for hybrid striped bass (HSB). The ideal model for formulation was the amino acid pattern of hybrid striped bass muscle. A negative control diet based on soybean (45%), corn gluten (10%), and poultry by-product (13%) meals was formulated on an ideal basis to contain 18% lipid, 40% crude protein (CP), 36% digestible protein (DP) and 1.8% Lys from intact sources. Met and Thr were then added at levels equivalent to 40% protein from HSB muscle to form a basal diet that was fortified with seven levels of Lys (2.2 to 6.4 g/g diet) that bracketed the predicted ideal Lys requirement (3.5 g/g diet) for this formulation. Diets were extruded to achieve similar characteristics as a commercial-grade feed and fed to juvenile fish (118 g BW) for 84 d. Response data were subjected to polynomial and exponential regression and the best model per response selected based on the lowest error (MSE) and p-values and the highest adjusted R-2. Selected models were used to derive dietary Lys levels required to reach 95% (R95) or 99% (R99) of the minimum or maximum response. Final fish weights (328-369 g) increased linearly with increasing dietary Lys. Weight gain, average daily feed intake (1.42-1.7%), and feed efficiencies at 4-, 8-, and 12-week intervals were modeled by cubic functions that yielded consistent R95 and R99 levels of about 2.4 and 4.3 g Lys/g diet, respectively. These values evenly bracketed the predicted ideal Lys requirement. Slightly higher Lys requirement was found for optimum FE at 4 weeks and smaller fish, as opposed to 8 or 12 weeks and larger fish. Whole body composition was unresponsive to diet Lys level; however, higher dietary Lys was required to minimize liver size (4.8 to 5.2 g Lys/g diet) than to minimize body fat (2.5 to 3.1 g Lys/g diet) or maximize muscle ratio (1.8 to 3.9 g Lys/g diet). Whole body protein, lipid, energy, and EAA retentions were also consistent with cubic functions that generally confirmed the predicted ideal Lys requirement with notable exceptions. Retention of Lys decreased exponentially with increasing dietary Lys, whereas, Met retention decreased in a linear fashion, corroborating that Met was first-limiting in this diet formula, as predicted. Retention of Thr and branched-chain amino acids were optimized at higher Lys levels (3.9-4.5 g/g diet) than those required to maximize growth parameters. Protein accretion (g/fish/d) responded quadratically to Lys intake predicting maximum deposition at 0.10 to 0.16 g Lys/fish/d. Histological assessment of intestines in fish fed these high-soybean meal test diets did not reveal any lesions associated with enteritis for any diet and overall normal intestinal morphology was observed in all fish sampled. Published by Elsevier B.V. C1 [Rawles, Steven D.; Fuller, S. Adam; Beck, Benjamin H.; McEntire, Matthew E.] USDA, ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Gaylord, T. Gibson] Bozeman Fish Technol Ctr, US Dept Interior, Fish & Wildlife Serv, Bozeman, MT 59715 USA. [Barrows, Frederic T.] USDA, ARS, Small Grains & Potato Germplasm Res Unit, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. RP Rawles, SD (reprint author), USDA, ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM steven.rawles@ars.usda.gov FU USDA/ARS [6225-31630-006-00D] FX Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This study was funded by the USDA/ARS under project number 6225-31630-006-00D. USDA is an equal opportunity provider and employer. NR 62 TC 4 Z9 4 U1 0 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD JUN 1 PY 2013 VL 396 BP 89 EP 101 DI 10.1016/j.aquaculture.2013.02.014 PG 13 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 139AK UT WOS:000318553500015 ER PT J AU Marchand, PA Straus, DL Wienke, A Pedersen, LF Meinelt, T AF Marchand, Pierre-Andre Straus, David L. Wienke, Andreas Pedersen, Lars-Flemming Meinelt, Thomas TI Effect of water hardness on peracetic acid toxicity to zebrafish, Danio rerio, embryos SO AQUACULTURE INTERNATIONAL LA English DT Article DE Peracetic acid; Toxicity; Water hardness; Zebrafish embryos; pH ID ICHTHYOPHTHIRIUS-MULTIFILIIS; DISINFECTION; PAA; THERONTS AB The use of peracetic acid (PAA) in aquaculture has been suggested as an alternative therapeutic agent. Few data are available concerning fish toxicity by PAA or factors that modify this toxicity. The aim of this study was to investigate the influence of water hardness on the acute toxicity of PAA products to embryos of zebrafish (Danio rerio). Embryos were exposed to PAA ranging from 0 to 9 mg/L in low-hardness (1.4 A degrees dH or 25 mg/L hardness as CaCO3), medium-hardness (14 A degrees dH or 250 mg/L hardness as CaCO3) and high-hardness (140 A degrees dH or 2,500 mg/L hardness as CaCO3) waters. The lowest LC50 value was 2.24 mg/L PAA in the low-hardness water, and the highest LC50 value was 7.14 mg/L PAA in the high-hardness water. Toxicity of PAA to embryos was found to be negatively correlated with water hardness. The pH decreased with increasing concentrations of PAA, and the test waters were observed to become more acidic in low hardness. In conclusion, aquaculturists using PAA should pay attention to water hardness to avoid acidosis. C1 [Marchand, Pierre-Andre] Catholic Univ Louvain, B-1348 Louvain, Belgium. [Straus, David L.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture, Stuttgart, AR USA. [Wienke, Andreas] Univ Halle Wittenberg, Halle Wittenberg Med Fac, Inst Med Epidemiol Biostat & Informat, D-06108 Halle, Germany. [Pedersen, Lars-Flemming] Tech Univ Denmark, North Sea Res Ctr 20, Aquaculture Sect, DTU Aqua, DK-9850 Hirtshals, Denmark. [Meinelt, Thomas] Leibniz Inst Freshwater Ecol & Inland Fisheries, D-12587 Berlin, Germany. RP Meinelt, T (reprint author), Leibniz Inst Freshwater Ecol & Inland Fisheries, Muggelseedamm 301, D-12587 Berlin, Germany. EM meinelt@igb-berlin.de RI Pedersen, Lars-Flemming/D-9919-2011 OI Pedersen, Lars-Flemming/0000-0002-8361-9268 FU Schreiner-Stiftung FX We wish to thank the Schreiner-Stiftung for financial support. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the Leibniz-Institute of Freshwater Ecology and Inland Fisheries or the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 17 TC 2 Z9 2 U1 1 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-6120 J9 AQUACULT INT JI Aquac. Int. PD JUN PY 2013 VL 21 IS 3 BP 679 EP 686 DI 10.1007/s10499-012-9602-9 PG 8 WC Fisheries SC Fisheries GA 137OO UT WOS:000318445500013 ER PT J AU Lee, SR Yates, SR Robarge, WP Bradford, SA AF Lee, Sang R. Yates, Scott R. Robarge, Wayne P. Bradford, Scott A. TI Synergistic ammonia losses from animal wastewater SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Ammonia; Carbon dioxide; Bicarbonate; Emissions; Thin-layer model; Swine lagoon; Dairy waste; Henry's law constant ID SOUTHEAST UNITED-STATES AB Thin-layer models are commonly used to estimate NH3 emissions from liquid waste. However, such models differ in their ability to accurately reproduce observed emissions, which may be partly due to an incomplete mechanistic understanding of NH3 volatilization. In this study, NH3 release from pure solutions of (NH4)(2)SO4 (AS), swine pit liquid (PL), swine lagoon liquid (LL), dairy lagoon liquid (DLL), and dairy manure liquid (DML) were evaluated under controlled conditions (pH 7.6 and temperature 20 degrees C). Relationships between the NH3 release and pH, temperature, and total ammoniacal nitrogen (TAN) were established. Under similar TAN conditions, the NH3 release was higher for PL, LL, DLL, and DML than for AS. Pure solutions of AS that were spiked with NaHCO3 showed NH3 emissions rates that were comparable to DML, DLL, LL and PL The enhanced NH3 emissions of PL, LL, DLL and DML were therefore explained by linkages between TAN and HCO3-. Published by Elsevier Ltd. C1 [Lee, Sang R.; Yates, Scott R.; Bradford, Scott A.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Robarge, Wayne P.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. RP Lee, SR (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM soilsang@gmail.com RI Lee, Sang Ryong/C-2167-2017 OI Lee, Sang Ryong/0000-0002-8672-301X NR 25 TC 2 Z9 2 U1 1 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 J9 ATMOS ENVIRON JI Atmos. Environ. PD JUN PY 2013 VL 71 BP 245 EP 250 DI 10.1016/j.atmosenv.2013.01.046 PG 6 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 136SY UT WOS:000318384900028 ER PT J AU Livingston, MJ Mishra, AK AF Livingston, Michael J. Mishra, Ashok K. TI Risk attitudes and premiums of US corn and soybean producers: an empirical investigation SO EMPIRICAL ECONOMICS LA English DT Article DE Risk attitudes; Risk premiums; Coefficient of absolute risk aversion; Coefficient of absolute downside risk aversion; Coefficient of relative risk aversion; Government payments; Agricultural Resource Management Survey ID RANDOM COEFFICIENT MODEL AB The 2000 Agricultural Risk Protection Act and 2002 Farm Security and Rural Investment Act reduced price and yield risks faced by many U.S. crop producers to low levels. We use a non-structural methodology extended for application to pseudo panels and national survey data to examine the risk attitudes of U.S. corn and soybean producers to test whether, and examine how, risk attitudes varied during the 1996-2001 and 2002-2008 periods by revenue category. We cannot reject the hypothesis of risk neutrality for the entire population, and for each revenue category, for the former period, but can reject risk neutrality, in favor of risk tolerance, for the entire population and for the larger revenue categories for the latter period. Estimated risk premiums for the latter period suggest that U.S. corn and soybean farmers who earn more require larger payments to remain indifferent between receiving their expected income with certainty and receiving an uncertain income from farming and government programs. C1 [Livingston, Michael J.] Econ Res Serv, USDA, Washington, DC 20520 USA. [Mishra, Ashok K.] Louisiana State Univ AgCtr, Dept Agr Econ & Agribusiness, Baton Rouge, LA 70803 USA. RP Livingston, MJ (reprint author), Econ Res Serv, USDA, 1400 Independence Ave SW,Mailstop 1800, Washington, DC 20520 USA. EM mlivingston@ers.usda.gov; Amishra@lsu.edu OI Mishra, Ashok/0000-0002-0988-1428 NR 24 TC 2 Z9 2 U1 0 U2 9 PU PHYSICA-VERLAG GMBH & CO PI HEIDELBERG PA PO BOX 10 52 80, 69042 HEIDELBERG, GERMANY SN 0377-7332 J9 EMPIR ECON JI Empir. Econ. PD JUN PY 2013 VL 44 IS 3 BP 1337 EP 1351 DI 10.1007/s00181-012-0580-6 PG 15 WC Economics; Social Sciences, Mathematical Methods SC Business & Economics; Mathematical Methods In Social Sciences GA 139AI UT WOS:000318553300016 ER PT J AU McCormack, ML Eissenstat, DM Prasad, AM Smithwick, EAH AF McCormack, M. Luke Eissenstat, David M. Prasad, Anantha M. Smithwick, Erica A. H. TI Regional scale patterns of fine root lifespan and turnover under current and future climate SO GLOBAL CHANGE BIOLOGY LA English DT Article DE belowground; carbon allocation; DISTRIB; dynamic global vegetation model (DGVM); ecological modeling; minirhizotron; tree distribution ID GLOBAL VEGETATION MODEL; SOIL CARBON; NITROGEN AVAILABILITY; FOREST ECOSYSTEMS; PRIMARY PRODUCTIVITY; ATMOSPHERIC CO2; LOBLOLLY-PINE; ELEVATED CO2; DYNAMICS; MORTALITY AB Fine root dynamics control a dominant flux of carbon from plants and into soils and mediate potential uptake and cycling of nutrients and water in terrestrial ecosystems. Understanding of these patterns is needed to accurately describe critical processes like productivity and carbon storage from ecosystem to global scales. However, limited observations of root dynamics make it difficult to define and predict patterns of root dynamics across broad spatial scales. Here, we combine species-specific estimates of fine root dynamics with a model that predicts current distribution and future suitable habitat of temperate tree species across the eastern United States (US). Estimates of fine root lifespan and turnover are based on empirical observations and relationships with fine root and whole-plant traits and apply explicitly to the fine root pool that is relatively short-lived and most active in nutrient and water uptake. Results from the combined model identified patterns of faster root turnover rates in the North Central US and slower turnover rates in the Southeastern US. Portions of Minnesota, Ohio, and Pennsylvania were also predicted to experience >10% increases in root turnover rates given potential shifts in tree species composition under future climate scenarios while root turnover rates in other portions of the eastern US were predicted to decrease. Despite potential regional changes, the average estimates of root lifespan and turnover for the entire study area remained relatively stable between the current and future climate scenarios. Our combined model provides the first empirically based, spatially explicit, and spatially extensive estimates of fine root lifespan and turnover and is a potentially powerful tool allowing researchers to identify reasonable approximations of forest fine root turnover in areas where no direct observations are available. Future efforts should focus on reducing uncertainty in estimates of root dynamics by better understanding how climate and soil factors drive variability in root dynamics of different species. C1 [McCormack, M. Luke; Eissenstat, David M.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [McCormack, M. Luke; Eissenstat, David M.; Smithwick, Erica A. H.] Penn State Univ, Intercoll Grad Degree Program Ecol, University Pk, PA 16802 USA. [Prasad, Anantha M.] US Forest Serv, Northeastern Res Stn, USDA, Delaware, OH 43015 USA. [Smithwick, Erica A. H.] Penn State Univ, Dept Geog, University Pk, PA 16802 USA. RP McCormack, ML (reprint author), Chinese Acad Sci, Key Lab Ecosyst Network Observat & Modeling, Synth Res Ctr, Chinese Ecosyst Res Network,Inst Geog Sci & Nat R, Beijing 100101, Peoples R China. EM mltmcc@gmail.com OI Smithwick, Erica/0000-0003-3497-2011 FU U.S. Department of Energy Office of Science (NICCR) [3819]; U.S. National Science Foundation [IOS0824704, ARC1107381]; U.S. Department of Energy Graduate GREF; LEAPS lab FX The authors would like to thank E. Crisfield and the LEAPS lab for support and expertise and helpful comments from three anonymous reviewers. This work was supported by the U.S. Department of Energy Office of Science (NICCR Award 3819) to EAHS, the U.S. National Science Foundation (Grants IOS0824704 and ARC1107381) to DME and the U.S. Department of Energy Graduate GREF to MLM. NR 62 TC 19 Z9 21 U1 9 U2 110 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2013 VL 19 IS 6 BP 1697 EP 1708 DI 10.1111/gcb.12163 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 136HP UT WOS:000318353300005 ER PT J AU Dijkstra, FA Morgan, JA Follett, RF Lecain, DR AF Dijkstra, Feike A. Morgan, Jack A. Follett, Ronald F. Lecain, Daniel R. TI Climate change reduces the net sink of CH4 and N2O in a semiarid grassland SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climate change; global warming potential; multifactor experiment; PHACE; positive feedback; water availability ID TRACE GAS FLUXES; ATMOSPHERIC CO2 ENRICHMENT; NITROUS-OXIDE FLUXES; ELEVATED CO2; CARBON-DIOXIDE; METHANE CONSUMPTION; FOREST SOIL; LAND-USE; EMISSIONS; TEMPERATURE AB Atmospheric concentrations of methane (CH4) and nitrous oxide (N2O) have increased over the last 150years because of human activity. Soils are important sources and sinks of both potent greenhouse gases where their production and consumption are largely regulated by biological processes. Climate change could alter these processes thereby affecting both rate and direction of their exchange with the atmosphere. We examined how a rise in atmospheric CO2 and temperature affected CH4 and N2O fluxes in a well-drained upland soil (volumetric water content ranging between 6% and 23%) in a semiarid grassland during five growing seasons. We hypothesized that responses of CH4 and N2O fluxes to elevated CO2 and warming would be driven primarily by treatment effects on soil moisture. Previously we showed that elevated CO2 increased and warming decreased soil moisture in this grassland. We therefore expected that elevated CO2 and warming would have opposing effects on CH4 and N2O fluxes. Methane was taken up throughout the growing season in all 5years. A bell-shaped relationship was observed with soil moisture with highest CH4 uptake at intermediate soil moisture. Both N2O emission and uptake occurred at our site with some years showing cumulative N2O emission and other years showing cumulative N2O uptake. Nitrous oxide exchange switched from net uptake to net emission with increasing soil moisture. In contrast to our hypothesis, both elevated CO2 and warming reduced the sink of CH4 and N2O expressed in CO2 equivalents (across 5years by 7% and 11% for elevated CO2 and warming respectively) suggesting that soil moisture changes were not solely responsible for this reduction. We conclude that in a future climate this semiarid grassland may become a smaller sink for atmospheric CH4 and N2O expressed in CO2-equivalents. C1 [Dijkstra, Feike A.] Univ Sydney, Dept Environm Sci, Fac Agr & Environm, Camden, NSW 2570, Australia. [Dijkstra, Feike A.] Univ Sydney, Ctr Carbon Water & Food, Fac Agr & Environm, Camden, NSW 2570, Australia. [Morgan, Jack A.; Lecain, Daniel R.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Follett, Ronald F.] USDA ARS, Soil Plant & Nutr Res Unit, Ft Collins, CO 80526 USA. RP Dijkstra, FA (reprint author), Univ Sydney, Dept Environm Sci, Fac Agr & Environm, Camden, NSW 2570, Australia. EM feike.dijkstra@sydney.edu.au OI Dijkstra, Feike/0000-0002-6191-6018 FU USDA-ARS; USDA-CSREES [2008-35107-18655]; US Department of Energy's Office of Science (BER); NSF [1021559]; Australian Research Council [FT100100779] FX We thank Erik Hardy, Valerie O'Neill, David Smith, Mary Smith, Katie Tylka, Megan Nix, and Ally Eden for technical assistance. This project was supported by the USDA-ARS Climate Change, Soils & Emissions and GRACEnet Programs, a USDA-CSREES Soil Processes Program (2008-35107-18655), the US Department of Energy's Office of Science (BER), and by NSF (DEB# 1021559). FAD acknowledges support from the Australian Research Council (FT100100779). NR 54 TC 21 Z9 24 U1 5 U2 153 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2013 VL 19 IS 6 BP 1816 EP 1826 DI 10.1111/gcb.12182 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 136HP UT WOS:000318353300014 PM 23505264 ER PT J AU Gomez-Casanovas, N Anderson-Teixeira, K Zeri, M Bernacchi, CJ DeLucia, EH AF Gomez-Casanovas, Nuria Anderson-Teixeira, Kristina Zeri, Marcelo Bernacchi, Carl J. DeLucia, Evan H. TI Gap filling strategies and error in estimating annual soil respiration SO GLOBAL CHANGE BIOLOGY LA English DT Article DE calculation error; carbon cycle; measurement; temporal extrapolation ID NET ECOSYSTEM EXCHANGE; TEMPERATURE-DEPENDENCE; CLIMATE-CHANGE; TERRESTRIAL ECOSYSTEMS; ELEVATED CO2; CARBON; FOREST; DECOMPOSITION; VEGETATION; MOISTURE AB Soil respiration (Rsoil) is one of the largest CO2 fluxes in the global carbon (C) cycle. Estimation of annual Rsoil requires extrapolation of survey measurements or gap filling of automated records to produce a complete time series. Although many gap filling methodologies have been employed, there is no standardized procedure for producing defensible estimates of annual Rsoil. Here, we test the reliability of nine different gap filling techniques by inserting artificial gaps into 20 automated Rsoil records and comparing gap filling Rsoil estimates of each technique to measured values. We show that although the most commonly used techniques do not, on average, produce large systematic biases, gap filling accuracy may be significantly improved through application of the most reliable methods. All methods performed best at lower gap fractions and had relatively high, systematic errors for simulated survey measurements. Overall, the most accurate technique estimated Rsoil based on the soil temperature dependence of Rsoil by assuming constant temperature sensitivity and linearly interpolating reference respiration (Rsoil at 10 degrees C) across gaps. The linear interpolation method was the second best-performing method. In contrast, estimating Rsoil based on a single annual Rsoil Tsoil relationship, which is currently the most commonly used technique, was among the most poorly-performing methods. Thus, our analysis demonstrates that gap filling accuracy may be improved substantially without sacrificing computational simplicity. Improved and standardized techniques for estimation of annual Rsoil will be valuable for understanding the role of Rsoil in the global C cycle. C1 [Gomez-Casanovas, Nuria; Anderson-Teixeira, Kristina; Bernacchi, Carl J.; DeLucia, Evan H.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [Gomez-Casanovas, Nuria; Anderson-Teixeira, Kristina; DeLucia, Evan H.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Zeri, Marcelo] Inst Nacl Pesquisas Espaciais, Ctr Ciencia Sistema Terr, BR-12630000 Cachoeira Paulista, SP, Brazil. [Bernacchi, Carl J.; DeLucia, Evan H.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP DeLucia, EH (reprint author), Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. EM delucia@life.illinois.edu RI Zeri, Marcelo/B-7701-2008; OI Zeri, Marcelo/0000-0003-1244-0858; Bernacchi, Carl/0000-0002-2397-425X FU Energy Biosciences Institute (EBI; University of Illinois at Urbana-Champaign, Urbana, IL, USA) FX This project was funded by the Energy Biosciences Institute (EBI; University of Illinois at Urbana-Champaign, Urbana, IL, USA). We thank Michael Masters for assistance in maintaining the automated Rsoil systems and field sampling. We are also grateful to Tim Mies, Chris Rudisill, Drew Schlumpf and Emily Doherty for operation of the Energy Farm at the University of Illinois, Urbana-Champaign. NR 47 TC 19 Z9 20 U1 0 U2 69 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUN PY 2013 VL 19 IS 6 BP 1941 EP 1952 DI 10.1111/gcb.12127 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 136HP UT WOS:000318353300025 PM 23504959 ER PT J AU Hall, DG Richardson, ML AF Hall, D. G. Richardson, M. L. TI Toxicity of insecticidal soaps to the Asian citrus psyllid and two of its natural enemies SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Cycloneda sanguinea; Diaphorina citri; M-Pede; Safer Insecticidal Soap; Tamarixia radiata AB The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an important pest of citrus because it transmits plant pathogens responsible for a serious disease of citrus known as huanglongbing. Conventional insecticides are frequently used to manage ACP. Insecticidal soaps (hereafter soaps') are an insect control option labelled for commercial use as well as for use by homeowners and organic growers. Soaps have been shown to be toxic to some insect pests and therefore might be an alternative to conventional pesticides for control of ACP, but the efficacy of soaps against ACP was largely unknown. Our objective was to test whether different concentrations of two insecticidal soaps, M-Pede and Safer Insecticidal Soap Concentrate, caused mortality of ACP adults, nymphs and eggs. In addition, we tested whether these soaps were toxic to two natural enemies of ACP, adults of the lady beetle Cycloneda sanguinea (L.) (Coleoptera: Coccinellidae) and the parasitoid Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae). Direct sprays of M-Pede or Safer Insecticidal Soap were acutely toxic to ACP adults (regardless of gender) and nymphs when applied in solutions of 0.82% in water. Insecticidal soaps were non-toxic to eggs at rates of up to 2%. Residues of soap were less toxic to adult ACP than direct sprays, even when applied at concentrations of up to 4%. M-Pede or Safer soap at high concentrations (for example, 2% v/v in water) may be an effective alternative to conventional pesticides to manage adult and nymphal ACP, although multiple applications may be needed if a target population includes eggs. A 2% concentration of either soap applied as a direct spray was non-toxic to adult C.sanguinea but acutely toxic to adult T.radiata. Soaps therefore may be compatible with biological control of ACP by adult coccinellids but not the parasitoid T.radiata. C1 [Hall, D. G.; Richardson, M. L.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Hall, DG (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM David.Hall@ars.usda.gov FU USDA-APHIS-PPQ FX We extend thanks to M. Hentz, P. Li, J. Malicoate, K. Moulton and M. Watson (USDA-ARS) for their assistance in the laboratory; to R. Sequeira (USDA-APHIS-PPQ) for partially funding the research; and to T. Meade (Dow AgroSciences) for reviewing a draft of the manuscript. This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U. S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. NR 0 TC 11 Z9 12 U1 8 U2 67 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUN PY 2013 VL 137 IS 5 BP 347 EP 354 DI 10.1111/j.1439-0418.2012.01749.x PG 8 WC Entomology SC Entomology GA 140ZS UT WOS:000318695600004 ER PT J AU Kennedy, JR Goodrich, DC Unkrich, CL AF Kennedy, Jeffrey R. Goodrich, David C. Unkrich, Carl L. TI Using the KINEROS2 Modeling Framework to Evaluate the Increase in Storm Runoff from Residential Development in a Semiarid Environment SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Storm-water management; Runoff; Infiltration; Hydrologic models ID TENSION INFILTROMETER; INFILTRATION-RATE; CATCHMENT; PATTERNS; SCALE; BASIN; WATER AB The increase in runoff from urbanization is well known; one extreme example comes from a 13-ha residential neighborhood in southeast Arizona where runoff was 26 times greater than in an adjacent grassland watershed over a 40-month period from 2005 to 2008. Rainfall-runoff modeling using the newly described KINEROS2 urban element, which simulates a contiguous row of houses and the adjoining street as a series of pervious and impervious overland flow planes, combined with tension infiltrometer measurements of saturated hydraulic conductivity (K-s), indicate that 17 +/- 14% of this increase in runoff is due to a 53% decrease in K-s in constructed pervious areas as compared to the undeveloped grassland. K-s in the urban watershed identified from calibrating the rainfall-runoff model to measured runoff is higher than measured K-s but much lower than indicated by a soil texture-based KINEROS2 parameter look-up table. Tests using different levels of discretization found that watershed geometry could be represented in a simplified manner, although more detailed discretization led to better model performance. DOI: 10.1061/(ASCE)HE.1943-5584.0000655. (C) 2013 American Society of Civil Engineers. C1 [Kennedy, Jeffrey R.] USGS, Tucson, AZ 85719 USA. [Goodrich, David C.; Unkrich, Carl L.] ARS, USDA, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. RP Kennedy, JR (reprint author), USGS, 520 N Pk Ave, Tucson, AZ 85719 USA. EM jkennedy@usgs.gov FU Upper San Pedro Partnership FX This study was supported by the Upper San Pedro Partnership, a consortium of public and private entities working to achieve sustainable groundwater yield in the Upper San Pedro basin. Tobias Finke and Jessica Kashian helped with infiltrometer measurements and laboratory particle-size analysis. Field offices of the USGS Arizona Water Science Center and the USDA-ARS Southwest Watershed Research Center collected rainfall, soil-moisture, and runoff data. Suggestions from journal reviewers considerably improved the manuscript. The Monte Carlo Analysis Toolbox by Thorsten Wagener was helpful in evaluating model performance. NR 40 TC 5 Z9 5 U1 1 U2 14 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 J9 J HYDROL ENG JI J. Hydrol. Eng. PD JUN PY 2013 VL 18 IS 6 BP 698 EP 706 DI 10.1061/(ASCE)HE.1943-5584.0000655 PG 9 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 139GQ UT WOS:000318570400008 ER PT J AU Ley, TW Allen, RG Jensen, ME AF Ley, Thomas W. Allen, Richard G. Jensen, Marvin E. TI Translating Wind-Speed Measurements over Alfalfa Having Varying Height for Use in the ASCE Standardized Reference ET Equation SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Article DE ASCE standardized reference evapotranspiration equation; Reference ET; Alfalfa; Wind speed; Agricultural weather station; Logarithmic wind-speed profile AB The ASCE Standardized Reference Evapotranspiration (ET) Equation expects wind speed measured at a height of 2 m over and downwind of a smooth measurement surface, such as actively growing clipped grass. Many agricultural weather stations used for evapotranspiration estimation may not meet such siting conditions. A physically based approach to translate wind speeds measured at various heights and weather measurement surface conditions to equivalent wind speed at 2-m height over actively growing clipped grass is tested in agricultural settings. The translation algorithm assumes neutral or near-neutral boundary layer stability conditions. Mean daily wind speeds measured at 2- and 3-m heights above ground surface over and downwind of variable-height alfalfa during the 2008, 2009, and 2010 growing seasons in several southeast Colorado locations were translated to equivalent mean daily wind speed at 2-m height over clipped grass and compared. Translated alfalfa weather station mean daily wind speeds were also compared with mean daily wind speeds measured at 2-m height over 0.12-m grass for parts of the 2009 and 2010 seasons at two pairs of sites. The translation algorithm produced translated mean daily wind speeds in good agreement with wind speeds measured under the standard weather station condition. The effects on computed daily tall crop reference evapotranspiration are illustrated. (C) 2013 American Society of Civil Engineers. C1 [Ley, Thomas W.] Colorado Div Water Resources, Hydrog Branch, Pueblo, CO 81004 USA. [Allen, Richard G.] Univ Idaho, Res & Extens Ctr, Kimberly, ID 83341 USA. [Jensen, Marvin E.] USDA ARS, Natl Res Program, Ft Collins, CO 80523 USA. [Jensen, Marvin E.] Colorado State Univ, Ft Collins, CO 80523 USA. RP Ley, TW (reprint author), Colorado Div Water Resources, Hydrog Branch, 310 E Abriendo,Suite B, Pueblo, CO 81004 USA. EM thomas.ley@state.co.us; rallen@kimberly.uidaho.edu; MJensen419@aol.com FU Colorado Water Conservation Board FX The assistance of Lane Simmons, Anthony Gutierrez, and Adam Adame with weather station maintenance and field data collection is gratefully acknowledged. The Colorado Water Conservation Board provided partial funding support for dataloggers and anemometers used in this study. The support of CoAgMet to allow modification of two CoAgMet stations for the purposes of this study is appreciated. Long-term lysimeter and weather data collected by Dr. James L. Wright (USDA-ARS, retired) were instrumental in the original development of Eq. (2). NR 14 TC 0 Z9 0 U1 0 U2 12 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD JUN PY 2013 VL 139 IS 6 BP 463 EP 475 DI 10.1061/(ASCE)IR.1943-4774.0000570 PG 13 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA 139GU UT WOS:000318570800004 ER PT J AU An, J Zheng, FL Romkens, MJM Li, GF Yang, QS Wen, LL Wang, B AF An, Juan Zheng, Fenli Roemkens, Mathias J. M. Li, Guifang Yang, Qingsen Wen, Leilei Wang, Bin TI The role of soil surface water regimes and raindrop impact on hillslope soil erosion and nutrient losses SO NATURAL HAZARDS LA English DT Article DE Saturation condition; Seepage condition; Raindrop impact; Nutrient loss; Soil loss ID SEDIMENT TRANSPORT; PHOSPHORUS LOSS; CONSERVATION-TILLAGE; HYDRAULIC GRADIENTS; AGGREGATE STABILITY; SUBTROPICAL CHINA; SOLUTE TRANSPORT; OVERLAND-FLOW; SANDY SOILS; SOURCE AREA AB Few investigations have addressed the interaction between soil surface water regimes and raindrop impact on nutrient losses, especially under artesian seepage condition. A simulation study was conducted to examine the effects on nitrogen and phosphorus losses. Four soil surface water regimes were designed: free drainage, saturation with rainfall, artesian seepage without rainfall, and artesian seepage with rainfall. These water regimes were subjected to two surface treatments: with and without raindrop impact through placing nylon net over soil pan. The results showed saturation and seepage with rainfall conditions induced greater soil loss and nutrient losses than free drainage condition. Nutrient concentrations in runoff from artesian seepage without rainfall condition were 7.3-228.7 times those from free drainage condition. Nutrient losses by runoff from saturation and seepage with rainfall conditions increased by factors of 1.30-9.38 and 2.81-40.11 times, and the corresponding losses with eroded sediment by 1.37-7.67 and 1.75-9.0 times, respectively, relative to those from free drainage condition. Regardless of different soil surface water regimes, raindrop impact increased 20.90-94.0 % nutrient losses with eroded sediment by promoting soil loss, but it only significantly enhanced nutrient transport to runoff under free drainage condition. C1 [An, Juan; Zheng, Fenli; Li, Guifang] Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [An, Juan; Zheng, Fenli; Li, Guifang] Minist Water Resources, Yangling 712100, Shaanxi, Peoples R China. [An, Juan; Li, Guifang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Zheng, Fenli; Yang, Qingsen; Wen, Leilei; Wang, Bin] Northwest Agr & Forestry Univ, Yangling 712100, Shaanxi, Peoples R China. [Roemkens, Mathias J. M.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Zheng, FL (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. EM anjuan.student@163.com; flzh@ms.iswc.ac.cn OI Wang, Bin/0000-0002-0563-5668 FU national basic research program of China [2007CB407201]; fundation of state key laboratory of soil erosion and dryland framing on Loess Plateau FX This study was supported by national basic research program of China (grand no. 2007CB407201) and fundation of state key laboratory of soil erosion and dryland framing on Loess Plateau. NR 60 TC 6 Z9 9 U1 5 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X J9 NAT HAZARDS JI Nat. Hazards PD JUN PY 2013 VL 67 IS 2 BP 411 EP 430 DI 10.1007/s11069-013-0570-9 PG 20 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA 138FN UT WOS:000318494300014 ER PT J AU Thompson, MP Scott, J Kaiden, JD Gilbertson-Day, JW AF Thompson, Matthew P. Scott, Joe Kaiden, Jeffrey D. Gilbertson-Day, Julie W. TI A polygon-based modeling approach to assess exposure of resources and assets to wildfire SO NATURAL HAZARDS LA English DT Article DE Risk assessment; Exposure analysis; Burn probability modeling; FSim; Simulation ID WILDLAND FIRE; CROWN FIRE; RISK; MANAGEMENT; SPREAD AB Spatially explicit burn probability modeling is increasingly applied to assess wildfire risk and inform mitigation strategy development. Burn probabilities are typically expressed on a per-pixel basis, calculated as the number of times a pixel burns divided by the number of simulation iterations. Spatial intersection of highly valued resources and assets (HVRAs) with pixel-based burn probability estimates enables quantification of HVRA exposure to wildfire in terms of expected area burned. However, statistical expectations can mask variability in HVRA area burned across all simulated fires. We present an alternative, polygon-based formulation for deriving estimates of HVRA area burned. This effort enhances investigations into spatial patterns of fire occurrence and behavior by overlaying simulated fire perimeters with mapped HVRA polygons to estimate conditional distributions of HVRA area burned. This information can be especially useful for assessing risks where cumulative effects and the spatial pattern and extent of area burned influence HVRA response to fire. We illustrate our modeling approach and demonstrate application across real-world landscapes for two case studies: first, a comparative analysis of exposure and area burned across ten municipal watersheds on the Beaverhead-Deerlodge National Forest in Montana, USA, and second, fireshed delineation and exposure analysis of a geographically isolated and limited area of critical wildlife habitat on the Pike and San Isabel National Forests in Colorado, USA. We highlight how this information can be used to inform prioritization and mitigation decisions and can be used complementarily with more traditional pixel-based burn probability and fire intensity metrics in an expanded exposure analysis framework. C1 [Thompson, Matthew P.; Gilbertson-Day, Julie W.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59802 USA. [Scott, Joe] Pyrologix LLC, Missoula, MT 59801 USA. [Kaiden, Jeffrey D.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59807 USA. RP Thompson, MP (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59802 USA. EM mpthompson02@fs.fed.us FU National Fire Decision Support Center; Rocky Mountain Research Station FX The National Fire Decision Support Center and the Rocky Mountain Research Station supported this research. We would like to acknowledge the work of Don Helmbrecht, and the Beaverhead-Deerlodge National Forest, the Pike and San Isabel National Forests, and the Rocky Mountain Region for their contributions to these analyses. NR 41 TC 12 Z9 12 U1 1 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X J9 NAT HAZARDS JI Nat. Hazards PD JUN PY 2013 VL 67 IS 2 BP 627 EP 644 DI 10.1007/s11069-013-0593-2 PG 18 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA 138FN UT WOS:000318494300025 ER PT J AU Tabanca, N Ali, A Bernier, UR Khan, IA Kocyigit-Kaymakcioglu, B Oruc-Emre, EE Unsalan, S Rollas, S AF Tabanca, Nurhayat Ali, Abbas Bernier, Ulrich R. Khan, Ikhlas A. Kocyigit-Kaymakcioglu, Bedia Oruc-Emre, Emine E. Unsalan, Seda Rollas, Sevim TI Biting deterrence and insecticidal activity of hydrazide-hydrazones and their corresponding 3-acetyl-2,5-disubstituted-2,3-dihydro-1,3,4-oxadiazoles against Aedes aegypti SO PEST MANAGEMENT SCIENCE LA English DT Article DE hydrazide-hydrazones; 1,3,4-oxadiazoles; biting deterrent; repellent; larvicidal; Aedes aegypti ID CULICIDAE; DIPTERA; RESPONSES AB Background Taking into account the improvement in insecticidal activity by the inclusion of fluorine in the hydrazone moiety, the authors synthesized new 4-fluorobenzoic acid hydrazides and 3-acetyl-2,5-disubstituted-2,3-dihydro-1,3,4-oxadiazoles, substituting a phenyl group or a heteroaryl ring carrying one or two atoms of F, Cl and Br, and investigated their biting deterrent and larvicidal activities against Aedes aegypti for the first time. Results The compound 3-acetyl-5-(4-fluorophenyl)-2-[4-(dimethylamino)phenyl]-2,3-dihydro-1,3,4-oxadiazole (17) produced the highest biting deterrent activity (BDI = 1.025) against Ae. Aegypti, followed by 4-fluorobenzoic acid [(phenyl)methylene] hydrazide (1). These activity results were similar to those of N,N-diethyl-meta-toluamide (DEET), which showed a proportion not biting of 0.80.92. When compounds 1 and 17 were tested on cloth worn on human volunteers, compound 1 was not repellent for some volunteers until present in excess of 500 nmol cm2, while compound 17 was not repellent at the highest concentration tested (1685 nmol cm2). In the larvicidal screening bioassays, only compounds 10, 11, 12 and 17 showed 100% mortality at the highest screening dose of 100 ppm against Ae. aegypti larvae. Compounds 11 and 12 with LD50 values of 24.1 and 30.9 ppm showed significantly higher mortality than 10 (80.3 ppm) and 17 (58.7 ppm) at 24-h post-treatment. Conclusion The insecticidal and biting deterrent activities were correlated with the presence of a halogen atom on the phenyl or heteroaryl substituent of the hydrazone moiety. C1 [Tabanca, Nurhayat; Ali, Abbas; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Bernier, Ulrich R.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. [Khan, Ikhlas A.] King Saud Univ, Coll Pharm, Dept Pharmacognosy, Riyadh, Saudi Arabia. [Kocyigit-Kaymakcioglu, Bedia; Unsalan, Seda; Rollas, Sevim] Marmara Univ, Fac Pharm, Dept Pharmaceut Chem, Istanbul, Turkey. [Oruc-Emre, Emine E.] Gaziantep Univ, Fac Arts & Sci, Dept Chem, Gaziantep, Turkey. RP Tabanca, N (reprint author), Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. EM ntabanca@olemiss.edu FU USDA/ARS [56-6402-1-612]; Deployed War-Fighter Protection Research Program; US Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by USDA/ARS grant No. 56-6402-1-612 and a Deployed War-Fighter Protection Research Program grant funded by the US Department of Defense through the Armed Forces Pest Management Board. The authors thank Dr Maia Tsikolia, Natasha Elejalde, Nathan Newlon and Greg Allen (USDA-ARS, Gainesville) for their technical assistance. They also thank Dr James J Becnel (Mosquito and Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville) for supplying Ae. aegypti eggs. NR 20 TC 6 Z9 7 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD JUN PY 2013 VL 69 IS 6 BP 703 EP 708 DI 10.1002/ps.3424 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA 142LX UT WOS:000318800000006 PM 23117879 ER PT J AU Chen, CY Xie, QM Xue, Y Ji, J Chang, S Ma, JY Bi, YZ AF Chen, Cui-Ying Xie, Qing-Mei Xue, Yu Ji, Jun Chang, Shuang Ma, Jing-Yun Bi, Ying-Zuo TI Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Cytotoxic responses; Marek's disease virus; The bursa of Fabricius ID MAJOR HISTOCOMPATIBILITY COMPLEX; FAS-MEDIATED APOPTOSIS; KILLER CELL-ACTIVITY; GRANZYME-A; IMMUNE-RESPONSES; VIRAL-INFECTION; HOST RESPONSES; T-CELLS; DEATH; CHICKEN AB Cell-mediated cytotoxic responses are critical for control of Marek's disease virus (MDV) infection and tumour development. However, the mechanisms of virus clearance mediated by cytotoxic responses in the bursa of Fabricius of chickens during MDV infection are not fully understood. In this study, the host cytotoxic responses during MDV infection in the bursa were investigated by examining the expression of genes in the cell lysis pathways. Partial up-regulation existed in the expression of the important cytolytic molecule granzyme A (GzmA), Fas, NK lysin and DNA repair enzyme Ape1, whereas little or no expression appeared in other cytolytic molecules, including perforin (PFN) and Fas ligand (FasL), and molecules involved in DNA repair and apoptosis in the bursa during MDV infection. These results suggest that less sustained cytotoxic activities are generated in the bursa of MDV-infected chickens. The findings of this study provide a more detailed insight into the host cytotoxic responses to MDV infection. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Chen, Cui-Ying; Xie, Qing-Mei; Xue, Yu; Ji, Jun; Ma, Jing-Yun; Bi, Ying-Zuo] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Chang, Shuang] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU National Natural Science Foundation of China [31072152] FX We thank Yu Zhou of Beijing Institute of Animal Husbandry and Veterinary Science for supplying the China standard J-1 strain of MDV1. This research was supported by the National Natural Science Foundation of China (Grant no. 31072152). C. Chen and Q. Xie conceived, designed this study, performed the experiments, carried out all statistical analysis and wrote the manuscript. Y. Xue participated in virus preparation and animal experiments. J. Ji and S. Chang supplied essential advice and critical reviews of this manuscript. Q. Xie, J. Ma and Y. Bi conducted the research. All authors read and approved the final manuscript. All authors have declared that no conflict of interest exists. NR 51 TC 3 Z9 4 U1 0 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 J9 RES VET SCI JI Res. Vet. Sci. PD JUN PY 2013 VL 94 IS 3 BP 496 EP 503 DI 10.1016/j.rvsc.2012.10.014 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 136RH UT WOS:000318380600021 PM 23164636 ER PT J AU Tang, XC Price, D Praske, E Lee, SA Shattuck, MA Purvis-Roberts, K Silva, PJ Asa-Awuku, A Cocker, DR AF Tang, Xiaochen Price, Derek Praske, Eric Lee, Su Anne Shattuck, Morgan A. Purvis-Roberts, Kathleen Silva, Philip J. Asa-Awuku, Akua Cocker, David R., III TI NO3 radical, OH radical and O-3-initiated secondary aerosol formation from aliphatic amines SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Secondary aerosol; Amine; Salt formation; Relative humidity ID VOLATILE ORGANIC-COMPOUNDS; PARTICLE MASS ANALYZER; AIR-POLLUTION; CHEMICAL-COMPOSITION; HIGH-RESOLUTION; TRIMETHYLAMINE; SPECTROMETER; CHEMISTRY; GAS; ATMOSPHERE AB Aliphatic amines enter the atmosphere from a variety of sources, and exist in both gas and particle phases in the atmosphere. Similar to ammonia, amines can form inorganic salts through acid-base reactions. However, the atmospheric behavior of amines with atmospheric oxidants (e.g. the nitrate radical (NO3), the hydroxyl radical (OH), O-3) is still poorly understood. In this study, chamber experiments were conducted to explore the reaction between three aliphatic amines and HNO3/O-3/NO3/OH. Effects of water vapor were also explored by conducting experiments under different relative humidity conditions (RH<0.1% to similar to 40%). Results show that all three amines have a high potential to form secondary aerosol in reactions with NO3, and are affected by the presence of water vapor. DEA and BA are capable of forming a significant amount of stable inorganic salt at ppb level concentrations, while TMA tends to form mostly non-salt secondary organic aerosol under dry conditions. The OH photooxidation of amines has much lower secondary aerosol yield and is independent of relative humidity, while ozonolysis produced negligible amount of aerosol. Secondary aerosol from OH oxidation was composed of organic components only, due to the lack of acid source. This study shows that night time chemistry of aliphatic amines can produce secondary organic and inorganic aerosol mixtures, and the relative contribution of each component depends on the environment relative humidity. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Tang, Xiaochen; Price, Derek; Asa-Awuku, Akua; Cocker, David R., III] Univ Calif Riverside, Dept Chem & Environm Engn, Bourns Coll Engn, Riverside, CA 92521 USA. [Tang, Xiaochen; Price, Derek; Asa-Awuku, Akua; Cocker, David R., III] Bourns Coll Engn, Ctr Environm Res & Technol CE CERT, Riverside, CA 92507 USA. [Praske, Eric; Lee, Su Anne; Shattuck, Morgan A.; Purvis-Roberts, Kathleen] Claremont Mckenna Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Praske, Eric; Lee, Su Anne; Shattuck, Morgan A.; Purvis-Roberts, Kathleen] Pitzer Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Praske, Eric; Lee, Su Anne; Shattuck, Morgan A.; Purvis-Roberts, Kathleen] Scripps Coll, WM Keck Sci Dept, Claremont, CA 91711 USA. [Silva, Philip J.] USDA ARS, Bowling Green, KY 42104 USA. [Silva, Philip J.] Utah State Univ, Dept Chem & Biochem, Logan, UT 84322 USA. RP Cocker, DR (reprint author), Univ Calif Riverside, Dept Chem & Environm Engn, Bourns Coll Engn, CE CERT, 1084 Columbia Ave, Riverside, CA 92507 USA. EM dcocker@engr.ucr.edu RI Cocker, David/F-4442-2010; OI Cocker, David/0000-0002-0586-0769; Price, Derek/0000-0003-3693-1475 FU National Science Foundation [ATM 0849765]; W. M. Keck Foundation FX We acknowledge funding support from National Science Foundation (ATM 0849765) and W. M. Keck Foundation. Any opinions, findings, and conclusions expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 50 TC 9 Z9 10 U1 12 U2 113 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 J9 ATMOS ENVIRON JI Atmos. Environ. PD JUN PY 2013 VL 72 BP 105 EP 112 DI 10.1016/j.atmosenv.2013.02.024 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 135BW UT WOS:000318262000014 ER PT J AU Leu, SY Zhu, JY AF Leu, Shao-Yuan Zhu, J. Y. TI Substrate-Related Factors Affecting Enzymatic Saccharification of Lignocelluloses: Our Recent Understanding SO BIOENERGY RESEARCH LA English DT Article DE Enzymatic hydrolysis/saccharification; Pretreatment; Biofuel and biorefinery; Lignocelluloses; Cellulase enzymes; Lignin; Accessibility ID INDUCED FIBER HORNIFICATION; PRETREATED LODGEPOLE PINE; SULFUR-DIOXIDE ADDITION; ETHANOL-PRODUCTION; STEAM EXPLOSION; CORN STOVER; CELLULOSE ACCESSIBILITY; LIGNIN CONTENT; DILUTE-ACID; CELL WALL AB Enzymatic saccharification of cellulose is a key step in conversion of plant biomass to advanced biofuel and chemicals. Many substrate-related factors affect saccharification. Rather than examining the role of each individual factor on overall saccharification efficiency, this study examined how each factor affects the three basic processes of a heterogeneous biochemistry reaction: (1) substrate accessibility to cellulose-the roles of component removal and size reduction by pretreatments, (2) substrate and cellulase reactivity limited by component inhibition, and (3) reaction conditions-substrate-specific optimization. Our in-depth analysis of published literature work, especially those published in the last 5 years, explained and reconciled some of the conflicting results in literature, especially the relative importance of hemicellulose vs. lignin removal and substrate size reduction on enzymatic saccharification of lignocelluloses. We concluded that hemicellulose removal is more important than lignin removal for creating cellulase accessible pores. Lignin removal is important when alkaline-based pretreatment is used with limited hemicellulose removal. Partial delignification is needed to achieve satisfactory saccharification of lignocelluloses with high lignin content, such as softwood species. Rather than using passive approaches, such as washing and additives, controlling pretreatment or hydrolysis conditions, such as pH, to modify lignin surface properties can be more efficient for reducing or eliminating lignin inhibition to cellulase, leading to improved lignocellulose saccharification. C1 [Leu, Shao-Yuan; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. [Zhu, J. Y.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. RP Zhu, JY (reprint author), Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. EM jzhu@fs.fed.us RI Leu, Shao-Yuan/C-7286-2017 OI Leu, Shao-Yuan/0000-0001-7262-1453 FU Agriculture and Food Research Initiative Competitive grant [2011-68005-30416]; USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA) FX We would like acknowledge the financial support from the Agriculture and Food Research Initiative Competitive grant no. 2011-68005-30416, USDA National Institute of Food and Agriculture (NIFA), through the Northwest Advanced Renewables Alliance (NARA), that made the post-doctoral appointment of Leu at the US Forest Service (USFS), Forest Products Laboratory (FPL) possible. We would also like to acknowledge Novozymes North America for their constant support by complementary providing cellulase enzymes, Fred Matt of USFS-FPL for conducting detailed chemical composition analysis, Tom Kuster (USFS-FPL) and Prof. Kecheng Li of University of New Brunswick (Canada) for the SEM and FE-SEM work, and Dr. Scott McNeil and his colleagues at SAIC-Frederick of National Cancer Institute for TEM work. Lastly, but not the least, many past visiting students and scholars at Zhu's laboratory at FPL are acknowledged for their dedicated work that produced most of the results presented. NR 102 TC 93 Z9 96 U1 12 U2 153 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 405 EP 415 DI 10.1007/s12155-012-9276-1 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700001 ER PT J AU Uppalapati, SR Serba, DD Ishiga, Y Szabo, LJ Mittal, S Bhandari, HS Bouton, JH Mysore, KS Saha, MC AF Uppalapati, Srinivasa Rao Serba, Desalegn D. Ishiga, Yasuhiro Szabo, Les J. Mittal, Shipra Bhandari, Hem S. Bouton, Joseph H. Mysore, Kirankumar S. Saha, Malay C. TI Characterization of the Rust Fungus, Puccinia emaculata, and Evaluation of Genetic Variability for Rust Resistance in Switchgrass Populations SO BIOENERGY RESEARCH LA English DT Article DE Switchgrass rust; Puccinia emaculata; Host resistance; Genetic variability; Internal transcribed spacer (ITS); Panicum virgatum ID POLYMERASE-CHAIN-REACTION; PANICUM-VIRGATUM; MULTILINE CULTIVARS; BIOMASS YIELD; 1ST REPORT; DIVERSITY; GRASSES; ADAPTATION; PATHOGENS; MIXTURES AB Several fungal pathogens have been identified on ornamental and native stands of switchgrass (Panicum virgatum L.). Diseases of switchgrass, particularly rust, have been largely neglected and are likely to become the major limiting factor to biomass yield and quality, especially when monocultured over a large acreage. Based on teliospore morphology and internal transcribed spacer-based diagnostic primers, the rust pathogen collected from switchgrass research fields in Oklahoma was identified as Puccinia emaculata. Furthermore, to identify genetically diverse source(s) of rust resistance, several switchgrass genotypes from both upland (cv. 'Summer' and 'Cave-in-Rock') and lowland (cv. 'Alamo' and 'Kanlow') ecotypes were evaluated in Ardmore, Oklahoma during 2008 and 2009 and in growth chamber assays. Field and growth chamber evaluations revealed a high degree of genetic variation within and among switchgrass cultivars. In general, Alamo and Kanlow showed moderate resistance to P. emaculata, while Summer was highly susceptible. Distinct ecotypic variations for reactions to rust were also prevalent with the lowlands maintaining a high level of resistance. These results suggest the potential for improvement of rust resistance via the selection of resistant individuals from currently available cultivars. Further, the selection pressure on the pathogen would also be reduced by employing several rust resistant cultivars in production-scale situations. C1 [Serba, Desalegn D.; Bhandari, Hem S.; Bouton, Joseph H.; Saha, Malay C.] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK 73401 USA. [Ishiga, Yasuhiro; Mittal, Shipra; Mysore, Kirankumar S.] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA. [Szabo, Les J.] Agr Res Serv, Cereal Dis Lab, USDA, St Paul, MN 55108 USA. RP Saha, MC (reprint author), Samuel Roberts Noble Fdn Inc, Forage Improvement Div, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA. EM Srinivasa.R.Uppalapati@ind.dupont.com; mcsaha@noble.org RI Ishiga, Yasuhiro/H-3814-2011 OI Ishiga, Yasuhiro/0000-0001-8871-8882 FU Samuel Roberts Noble Foundation; Ceres Inc., CA; Department of Energy; National Science Foundation-Experimental Program to Stimulate Competitive Research [EPS-0814361] FX We thank Dr. Mark Sorrells for helpful discussions and encouragement and Drs. Kelly Craven and Seonghee Lee for critical reading of the manuscript. We also thank Ms. Karen Flower for fungicide treatments and plant rescue following rust infections. We are very grateful to Dr. Stephen Webb for his help in statistical analysis. This work was supported through funding support from The Samuel Roberts Noble Foundation, Ceres Inc., CA, and Bioenergy Science Center were supported by the Department of Energy. S.R.U also acknowledges financial support through a grant from National Science Foundation-Experimental Program to Stimulate Competitive Research (EPS-0814361) funded to K.S.M. NR 45 TC 10 Z9 10 U1 2 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 458 EP 468 DI 10.1007/s12155-012-9263-6 PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700005 ER PT J AU Lan, TQ Lou, HM Zhu, JY AF Lan, T. Q. Lou, Hongming Zhu, J. Y. TI Enzymatic Saccharification of Lignocelluloses Should be Conducted at Elevated pH 5.2-6.2 SO BIOENERGY RESEARCH LA English DT Article DE Enzymatic hydrolysis/saccharification; Hydrolysis pH; Pretreatment; Biofuel and biorefinery; Cellulase enzymes; Cellulase binding ID TRICHODERMA-REESEI; ETHANOL-PRODUCTION; LODGEPOLE-PINE; HYDROLYSIS; PRETREATMENT; CHROMATOGRAPHY; PURIFICATION; BIOFUELS; BIOMASS; LIGNIN AB This study revealed that cellulose enzymatic saccharification response curves of lignocellulosic substrates were very different from those of pure cellulosic substrates in terms of optimal pH and pH operating window. The maximal enzymatic cellulose saccharification of lignocellulosic substrates occurs at substrate suspension pH 5.2-6.2, not between pH 4.8 and 5.0 as exclusively used in literature using T. reesi cellulase. Two commercial cellulase enzyme cocktails, Celluclast 1.5L and CTec2 both from Novozymes, were evaluated over a wide range of pH. The optimal ranges of measured suspension pH of 5.2-5.7 for Celluclast 1.5L and 5.5-6.2 for CTec2 were obtained using six lignocellulosic substrates produced by dilute acid, alkaline, and two sulfite pretreatments to overcome recalcitrance of lignocelluloses (SPORL) pretreatments using both a softwood and a hardwood. Furthermore, cellulose saccharification efficiency of a SPORL-pretreated lodgepole pine substrate showed a very steep increase between pH 4.7 and 5.2. Saccharification efficiency can be increased by 80 % at cellulase loading of 11.3 FPU/g glucan, i.e., from approximately 43 to 78 % simply by increasing the substrate suspension pH from 4.7 to 5.2 (buffer solution pH from 4.8 to 5.5) using Celluclast 1.5L, or by 70 % from approximately 51 to 87 % when substrate suspension pH is increased from 4.9 to 6.2 (buffer solution pH from 5.0 to 6.5) using CTec2. The enzymatic cellulose saccharification response to pH is correlated to the degree of substrate lignin sulfonation. The difference in pH-induced lignin surface charge, and therefore surface hydrophilicity and lignin-cellulase electrostatic interactions, among different substrates with different lignin content and structure is responsible for the reported different enhancements in lignocellulose saccharification at elevated pH. C1 [Lan, T. Q.; Lou, Hongming; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Lan, T. Q.] S China Univ Technol, Sch Light Ind & Food Sci, Guangzhou, Guangdong, Peoples R China. [Lou, Hongming] S China Univ Technol, Sch Chem & Chem Engn, Guangzhou, Guangdong, Peoples R China. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. EM jzhu@fs.fed.us OI Lou, Hongming/0000-0003-3941-7287 FU USDA Small Business Innovative Research (SBIR) Phase II project [2010-33610-21589]; US Forest Service (USFS), Forest Products Laboratory (FPL) FX This work was partially supported by a USDA Small Business Innovative Research (SBIR) Phase II project (contract number 2010-33610-21589) to Biopulping International, Inc. The financial support from this project made the visiting appointment of Lan at the US Forest Service (USFS), Forest Products Laboratory (FPL) possible. We acknowledge Fred Matt and Kolby Hirth (both USFS-FPL) for carrying out the carbohydrate and sulfur content analyses, respectively. NR 28 TC 57 Z9 61 U1 4 U2 90 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 EI 1939-1242 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 476 EP 485 DI 10.1007/s12155-012-9273-4 PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700007 ER PT J AU Watrud, LS Reichman, JR Bollman, MA Smith, BM Lee, EH Jastrow, JD Casler, MD Collins, HP Fransen, S Mitchell, RB Owens, VN Bean, B Rooney, WL Tyler, DD King, GA AF Watrud, Lidia S. Reichman, Jay R. Bollman, Michael A. Smith, Bonnie M. Lee, E. Henry Jastrow, Julie D. Casler, Michael D. Collins, Harold P. Fransen, Steven Mitchell, Robert B. Owens, Vance N. Bean, Brent Rooney, William L. Tyler, Donald D. King, George A. TI Chemistry and Microbial Functional Diversity Differences in Biofuel Crop and Grassland Soils in Multiple Geographies SO BIOENERGY RESEARCH LA English DT Article DE Switchgrass; Sorghum; GeoChip; Functional gene array; Soil ecosystem services ID MICROARRAY-BASED ANALYSIS; RIBOSOMAL-RNA ANALYSIS; SEA OIL PLUME; BACTERIAL COMMUNITIES; FLUORESCEIN DIACETATE; ECOSYSTEM PROCESSES; SORGHUM-HALEPENSE; ANALYSIS REVEALS; PLANT; RHIZOSPHERE AB We obtained soil samples from geographically diverse switchgrass (Panicum virgatum L.) and sorghum (Sorghum bicolor L.) crop sites and from nearby reference grasslands and compared their edaphic properties, microbial gene diversity and abundance, and active microbial biomass content. We hypothesized that soils under switchgrass, a perennial, would be more similar to reference grassland soils than sorghum, an annual crop. Sorghum crop soils had significantly higher NO3 (-) -N, NH4 (+) -N, SO4 (2-) -S, and Cu levels than grassland soils. In contrast, few significant differences in soil chemistry were observed between switchgrass crop and grassland soils. Active bacterial biomass was significantly lower in sorghum soils than switchgrass soils. Using GeoChip 4.0 functional gene arrays, we observed that microbial gene diversity was significantly lower in sorghum soils than grassland soils. Gene diversity at sorghum locations was negatively correlated with NO3 (-) -N, NH4 (+) -N, and SO4 (2-) -S in C and N cycling microbial gene categories. Microbial gene diversity at switchgrass sites varied among geographic locations, but crop and grassland sites tended to be similar. Microbial gene abundance did not differ between sorghum crop and grassland soils, but was generally lower in switchgrass crop soils compared to grassland soils. Our results suggest that switchgrass has fewer adverse impacts on microbial soil ecosystem services than cultivation of an annual biofuel crop such as sorghum. Multi-year, multi-disciplinary regional studies comparing these and additional annual and perennial biofuel crop and grassland soils are recommended to help define sustainable crop production and soil ecosystem service practices. C1 [Watrud, Lidia S.; Reichman, Jay R.; Bollman, Michael A.; Smith, Bonnie M.; Lee, E. Henry] US EPA, Corvallis, OR 97333 USA. [Jastrow, Julie D.] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA. [Casler, Michael D.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Collins, Harold P.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Fransen, Steven] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Mitchell, Robert B.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Owens, Vance N.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Bean, Brent] Texas A&M Univ, Texas Agrilife Res & Extens Ctr, Amarillo, TX 79106 USA. [Rooney, William L.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Tyler, Donald D.] Univ Tennessee, West TN Res & Educ Ctr, Jackson, TN 38301 USA. [King, George A.] Dynamac Corp, Corvallis, OR 97333 USA. RP Watrud, LS (reprint author), US EPA, 200 SW 35th St, Corvallis, OR 97333 USA. EM watrud.lidia@epa.gov FU United States Environmental Protection Agency (USEPA) Office of Research and Development National Health and Environmental Effects Research Laboratory; EPA [EP-D-06-013, EP-D-11-027] FX The authors thank their respective staff who participated in providing soil samples, photographs, GPS coordinates, and other background information for each of the crop and non-crop sampling locations. This research was funded in part by a United States Environmental Protection Agency (USEPA) Office of Research and Development National Health and Environmental Effects Research Laboratory intramural competitive award to LSW and RJF and by EPA contracts to Dynamac Corporation (EP-D-06-013 and EP-D-11-027). Mention of trade names or commercial products does not imply endorsement for use. The views of the authors do not necessarily reflect those of the Agency. This manuscript has undergone administrative and technical reviews to receive Agency approval for submission for publication in a peer-reviewed scientific journal. NR 56 TC 2 Z9 2 U1 4 U2 55 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 601 EP 619 DI 10.1007/s12155-012-9279-y PG 19 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700017 ER PT J AU Toth, K Van Gool, MP Schols, HA Samuels, GJ Gruppen, H Szakacs, G AF Toth, Karolina Van Gool, Martine P. Schols, Henk A. Samuels, Gary J. Gruppen, Harry Szakacs, George TI Diversity in Production of Xylan-Degrading Enzymes Among Species Belonging to the Trichoderma Section Longibrachiatum SO BIOENERGY RESEARCH LA English DT Article DE Xylan; Hemicellulase; Biorefineries; Lignocellulosic biomass; Trichoderma ID WHEAT-FLOUR ARABINOXYLAN; INDUSTRIAL APPLICATIONS; H-1-NMR SPECTROSCOPY; ASPERGILLUS-AWAMORI; BETA-GLUCOSIDASE; BIOFUELS; REESEI; FERMENTATION; HYPOCREA; ENDO-BETA-1,4-XYLANASES AB Xylan is an important part of plant biomass and represents a renewable raw material for biorefineries. Contrary to cellulose, the structure of hemicellulose is quite complex. Therefore, the biodegradation of xylan needs the cooperation of many enzymes. For industrial production of xylanase multienzyme complexes (cocktails) and selected monocomponent xylanases, different Trichoderma reesei mutants and recombinants are used. T. reesei QM 6a (wild-type parent of best existing mutants) was selected as a starting material in the 1960s when the modern in-depth analytical methods were not yet in use. Therefore, screening of fungi genetically close to T. reesei in biodegradation of xylan may have a scientific value. Fifteen different strains from Trichoderma section Longibrachiatum have been tested for extracellular xylan-degrading enzyme production on three carbon sources (wheat straw, corn fiber, and eucalyptus wood) in shake flask cultivation. The enzyme activities were evaluated by traditional colorimetric enzyme assays and by HPLC and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Degradation of xylan was studied on four different xylan-rich model substrates. T. reesei CPK 155, Trichoderma parareesei TUB F-2535, and Trichoderma gracile TUB F-2543 isolates were equally good or better in degradation of the wheat arabinoxylan (WAX) and corn fiber alcohol insoluble solids as hydolysis substrates than the well-known T. reesei QM 6a and RUT C30 strains. Though Trichoderma saturnisporum ATCC 18903 gave relatively low volumetric enzyme activities by traditional colorimetric assays, it could release quite large amount of hydrolysis products (mono- and oligosaccharides) from WAX. Therefore, these fungi may be potential candidates for further experiments. Enzyme production on wheat straw and corn fiber carbon sources was more effective than on eucalyptus wood. C1 [Toth, Karolina; Szakacs, George] Budapest Univ Technol & Econ, Dept Appl Biotechnol & Food Sci, H-1111 Budapest, Hungary. [Van Gool, Martine P.; Schols, Henk A.; Gruppen, Harry] Wageningen Univ, Food Chem Lab, NL-6703 HD Wageningen, Netherlands. [Samuels, Gary J.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Szakacs, G (reprint author), Budapest Univ Technol & Econ, Dept Appl Biotechnol & Food Sci, Szent Gellert Ter 4, H-1111 Budapest, Hungary. EM gszakacs@mail.bme.hu OI van Gool, Martine/0000-0003-1895-2483 FU European Commission FX Majority of the work was carried out in the framework of the research project "Targeted DISCOvery of novel cellulases and hemicellulases and their reaction mechanisms for hydrolysis of lignocellulose biomass" (www.disco-project.eu). The authors are grateful to the European Commission for supporting this study. The contents of the publication reflects only the authors view. We thank Zsuzsanna Ferencz for the excellent technical assistance. NR 40 TC 2 Z9 2 U1 2 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 631 EP 643 DI 10.1007/s12155-012-9282-3 PG 13 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700019 ER PT J AU Cotton, J Burow, G Acosta-Martinez, V Moore-Kucera, J AF Cotton, Jon Burow, Gloria Acosta-Martinez, Veronica Moore-Kucera, Jennifer TI Biomass and Cellulosic Ethanol Production of Forage Sorghum Under Limited Water Conditions SO BIOENERGY RESEARCH LA English DT Article DE Biofuels; Ethanol; Forage sorghum; Ogallala aquifer; Simultaneous saccharification and fermentation; Water deficit; Deficit irrigation ID SOUTHERN HIGH-PLAINS; BICOLOR L. MOENCH; SACCHARIFICATION; FEEDSTOCK; BIOENERGY; NITROGEN; STOVER; SWEET; YIELD; CORN AB This study presents results from a 2-year evaluation of biomass and cellulosic ethanol (EtOH) production potential of forage sorghum (Sorghum bicolor L. Moench) cultivars differing in brown midrib trait (i.e., bmr12) under dryland (no irrigation) and limited irrigation (2.88 mm day(-1); subsurface drip) in the semiarid Southern High Plains of the USA. Commercial cultivar Sorghum Partners 1990 (SP 1990, conventional non-bmr) produced significantly more biomass (29-62 %) than a bmr12 cultivar PaceSetter bmr (PS bmr) under irrigated and dryland conditions during both years of this study. However, PS bmr biomass had higher cellulosic EtOH conversion efficiency than SP 1990 in both years according to simultaneous saccharification and fermentation analysis. Irrigation resulted in 26-49 % more biomass and 28-72 % more cellulosic EtOH production during both growing seasons, indicating that limited irrigation had favorable effects on both biomass and biofuel production. In the first year, when precipitation was below average, both cultivars produced similar amounts of cellulosic EtOH. During the second year, when precipitation was above average, higher biomass production of SP 1990 resulted in 28 % higher cellulosic EtOH production than PS bmr when averaged across both irrigated and dryland conditions. The large range of cellulosic EtOH production (1,600 to 3,380 L ha(-1)) during the 2 years of this study was primarily driven by differences in water availability that resulted from precipitation and irrigation. Our findings indicates that chemical composition and biomass yield potential of sorghum cultivars are critical factors that affect biomass and biofuel production under limited water conditions. C1 [Cotton, Jon; Burow, Gloria; Acosta-Martinez, Veronica] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Cotton, Jon; Moore-Kucera, Jennifer] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. RP Cotton, J (reprint author), Texas Tech Univ, Dept Plant & Soil Sci, Campus Box 42122, Lubbock, TX 79409 USA. EM Jon.Cotton@ars.usda.gov FU USDA-ARS Ogallala Aquifer Initiative FX We would like to thank the USDA-ARS Ogallala Aquifer Initiative for providing funding for this project, Dr. David Wester for input in statistical analysis, and laboratory personnel at the USDA-ARS for assistance in field and lab data collection. NR 33 TC 4 Z9 4 U1 5 U2 50 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 711 EP 718 DI 10.1007/s12155-012-9285-0 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700026 ER PT J AU Hendrickson, JR Schmer, MR Sanderson, MA AF Hendrickson, J. R. Schmer, M. R. Sanderson, M. A. TI Water Use Efficiency by Switchgrass Compared to a Native Grass or a Native Grass Alfalfa Mixture SO BIOENERGY RESEARCH LA English DT Article DE Drought; Western wheatgrass; Soil water deficit; Rainout shelter ID MEDICAGO-SATIVA L; FORAGE YIELD; WESTERN WHEATGRASS; CRESTED WHEATGRASS; TALL FESCUE; PRECIPITATION; RESPONSES; NITROGEN; CARBON; SOIL AB Perennial grass systems are being evaluated as a bioenergy feedstock in the northern Great Plains. Inter-annual and inter-seasonal precipitation variation in this region will require efficient water use to maintain sufficient yield production to support a mature bioenergy industry. Objectives were to evaluate the impact of a May-June (early season) and a July-August (late season) drought on the water use efficiency (WUE), amount of water used, and biomass production in monocultures of switchgrass (Panicum virgatum L.), western wheatgrass (Pascopyrum smithii (Rydb.) A. Love), and a western wheatgrass-alfalfa (Medicago sativa L.) mixture using an automated rainout shelter. WUE was strongly driven by biomass accumulation and ranged from 5.6 to 7.4 g biomass mm(-1) water for switchgrass to 1.06 to 2.07 g biomass mm(-1) water used with western wheatgrass. Timing of water stress affected WUE more in western wheatgrass and the western wheatgrass-alfalfa mixture than switchgrass. Water deficit for the western wheatgrass-alfalfa mixture was 23 % lower than western wheatgrass (P = 0.0045) and 31 % lower than switchgrass (P < 0.0001) under the May-June stress water treatment, while switchgrass had a 37 and 38 % greater water deficit than did western wheatgrass or western wheatgrass-alfalfa mixture, respectively (P < 0.001) under the July-August water stress treatment. Water depletion was always greatest in the upper 30 cm. Switchgrass had greater WUE but resulted in greater soil water depletion at the end of the growing season compared to western wheatgrass and a western wheatgrass-alfalfa mixture which may be a concern under multi-year drought conditions. C1 [Hendrickson, J. R.; Sanderson, M. A.] ARS, No Great Plains Res Lab, USDA, Mandan, ND 58554 USA. [Schmer, M. R.] Univ Nebraska, Agroecosyst Management Res Unit, USDA, ARS, Lincoln, NE 68583 USA. RP Hendrickson, JR (reprint author), ARS, No Great Plains Res Lab, USDA, POB 459, Mandan, ND 58554 USA. EM john.hendrickson@ars.usda.gov NR 45 TC 4 Z9 7 U1 2 U2 38 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 746 EP 754 DI 10.1007/s12155-012-9290-3 PG 9 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700030 ER PT J AU Wienhold, BJ Varvel, GE Johnson, JMF Wilhelm, WW AF Wienhold, B. J. Varvel, G. E. Johnson, J. M. F. Wilhelm, W. W. TI Carbon Source Quality and Placement Effects on Soil Organic Carbon Status SO BIOENERGY RESEARCH LA English DT Article DE Carbon sequestration; Soil structure; Plant residue; Soil aggregation ID DECOMPOSITION; REMOVAL; CROP AB Improved management of agricultural soils has potential for sequestering carbon (C) and reducing the accumulation of atmospheric carbon dioxide. Development of management practices to increase C sequestration is dependent on improved understanding of soil processes influencing long-term storage of C. A field study was conducted to compare surface C source quality and above- vs. belowground addition of annual or perennial plant biomass effects on particulate organic matter (POM), total labile C (TLC), and total organic C (TOC). Since microaggregate stabilization within macroaggregates is the main mechanism for sequestering C, aggregate size distribution, expressed as mean weight diameter (MWD), and wet aggregate stability (WAS) was also measured. After 5 years, POM decreased in plots receiving surface application of readily available substrate (sucrose and alfalfa pellets) and the bare surface control. Plots receiving plant additions [wood chips, growing wheat (Triticum aestivum L.) crop, growing switchgrass (Panicum virgatum L.) crop, and fallow receiving either wheat or switchgrass residue] exhibited higher TLC and TOC content. Plots receiving plant residue maintained MWD, and those supporting live plants exhibited increasing WAS. Surface plant residue protected the soil against raindrop impact and reduced the intensity of wetting and drying cycles allowing the development of larger more stable aggregates resulting in C accrual. C1 [Wienhold, B. J.; Varvel, G. E.; Wilhelm, W. W.] ARS, USDA, UNL, Lincoln, NE 68583 USA. [Johnson, J. M. F.] ARS, USDA, Morris, MN USA. RP Wienhold, BJ (reprint author), ARS, USDA, UNL, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM Brian.Wienhold@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 NR 21 TC 8 Z9 9 U1 2 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 786 EP 796 DI 10.1007/s12155-013-9301-z PG 11 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700034 ER PT J AU Washburn, JD Whitmire, DK Murray, SC Burson, BL Wickersham, TA Heitholt, JJ Jessup, RW AF Washburn, Jacob D. Whitmire, David K. Murray, Seth C. Burson, Byron L. Wickersham, Tryon A. Heitholt, James J. Jessup, Russell W. TI Estimation of Rhizome Composition and Overwintering Ability in Perennial Sorghum spp. Using Near-Infrared Spectroscopy (NIRS) SO BIOENERGY RESEARCH LA English DT Article DE Sorghum; Perennial; NIRS; Near-infrared spectroscopy; Rhizome composition ID HALEPENSE L PERS; DIFFERENTIAL COLD TOLERANCE; FREEZING TOLERANCE; GENETIC-IMPROVEMENT; JOHNSON GRASS; CARBOHYDRATE; FEEDSTOCK; CULTIVARS; CROPS; SUGAR AB Temperately adapted perennial sorghum feedstocks have recently begun to receive increasing interest as candidate energy crops, producing significant biomass and contributing agroecological benefits including increased soil organic carbon, reduced soil erosion, reduced input requirements, and higher net energy return. Rhizomes are the primary morphological feature facilitating overwintering in Sorghum species; however, underlying physiological mechanisms governing rhizome overwintering remain poorly characterized. In this study, we investigated the composition of sorghum rhizomes from diverse germplasm before and after overwintering at two locations and three experimental environments. Significant positive correlations were found between rhizome overwintering and water-soluble carbohydrates, ethanol soluble carbohydrates, and fructan concentrations, while significant negative correlations were found between rhizome overwintering and both crude fat and starch. Near-infrared spectroscopy (NIRS) calibration equations were developed to quickly and efficiently predict the concentrations of each of these assimilates in rhizomes. C1 [Washburn, Jacob D.; Whitmire, David K.; Murray, Seth C.; Jessup, Russell W.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Burson, Byron L.] ARS, USDA, Crop Germplasm Res Unit, College Stn, TX 77845 USA. [Wickersham, Tryon A.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Heitholt, James J.] Texas A&M Univ, Dept Agr Sci, Commerce, TX 75429 USA. RP Jessup, RW (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, 2474 TAMU, College Stn, TX 77843 USA. EM rjessup@neo.tamu.edu NR 45 TC 2 Z9 2 U1 1 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 822 EP 829 DI 10.1007/s12155-013-9305-8 PG 8 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700038 ER PT J AU Shah, SN Iha, OK Alves, FCSC Sharma, BK Erhan, SZ Suarez, PAZ AF Shah, Shailesh N. Iha, Osvaldo K. Alves, Flavio C. S. C. Sharma, Brajendra K. Erhan, Sevim Z. Suarez, Paulo A. Z. TI Potential Application of Turnip Oil (Raphanus sativus L.) for Biodiesel Production: Physical-Chemical Properties of Neat Oil, Biofuels and their Blends with Ultra-Low Sulphur Diesel (ULSD) SO BIOENERGY RESEARCH LA English DT Article DE Biodiesel; Turnip oil (Raphanus sativus L.); Non-food feedstock transesterification; Methanol; ULSD ID METHYL-ESTERS; FUEL PROPERTIES; VEGETABLE-OILS; SEED PROTEIN; COMPONENTS; PETRODIESEL; ADDITIVES; LUBRICITY; ALCOHOLS AB Turnip oil (TO; Raphanus sativus L.) produces seeds that contain around 26 wt% of inedible base stock that are suitable as a potential feedstock for biodiesel production. A turnip oil methyl ester (TME) was prepared from acid-catalyzed pretreated TO in an effort to evaluate important fuel properties of turnip oil-based biodiesel, such as kinematic viscosity, cloud point, pour point (PP), cold filter plugging point, acid value, oxidative stability and lubricity. A comparison was made with soybean oil methyl esters (SME) as per biodiesel fuel standards such as ASTM D6751 and EN 14214. TME was characterized using FTIR, HPLC and H-1 NMR. Except PP property, SME displays superior fuel properties compared to TME. Blends (B5 and B20) of TME in ultra-low sulphur diesel fuel (ULSD) were also assessed for the aforesaid fuel properties and compared to an analogous set of blends of soybean oil methyl ester in ULSD as per petro diesel fuel standards such as ASTM D975 and D7467. TME B5 blends in ULSD displayed improved PP property in comparison to neat ULSD and blends of SME in ULSD. It was demonstrated that the B5 and B20 blends of TME in ULSD had acceptable fuel properties as per ASTM D975 (for B5 blend) and ASTM D7467 (for B20 blend). In summary, turnip oil has potential as an alternative, non-food feedstock for biodiesel production. C1 [Shah, Shailesh N.; Sharma, Brajendra K.; Erhan, Sevim Z.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Iha, Osvaldo K.; Alves, Flavio C. S. C.; Suarez, Paulo A. Z.] Univ Brasilia, Lab Mat & Combust, Inst Quim, BR-70919970 Brasilia, DF, Brazil. [Sharma, Brajendra K.] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Erhan, Sevim Z.] ARS, USDA, EERC, Wyndmoor, PA 19038 USA. [Shah, Shailesh N.] Maharaja Sayajirao Univ Baroda, Fac Technol & Engn, Dept Appl Chem, Vadodara 390001, Gujarat, India. RP Shah, SN (reprint author), Maharaja Sayajirao Univ Baroda, Fac Technol & Engn, Dept Appl Chem, Post Box 51, Vadodara 390001, Gujarat, India. EM shilshilp@hotmail.com; psuarez@unb.br FU Research and Projects Financing (FINEP); National Counsel of Technological and Scientific Development (CNPq); Banco do Brasil Foundation (FBB); Federal District Research Support Foundation (FAPDF); CNPq FX The authors acknowledge financial support from Brazilian research founding agencies, such as Research and Projects Financing (FINEP), National Counsel of Technological and Scientific Development (CNPq), Banco do Brasil Foundation (FBB) and Federal District Research Support Foundation (FAPDF). OKH, FCSCA and PAZS thank CNPq for research fellowships. The authors acknowledge Bryan Moser and Jill K. Winkler-Moser for acquisition of phytosterol and tocopherol data and also Erin L. Walter for excellent technical assistance. NR 34 TC 5 Z9 5 U1 1 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-1234 J9 BIOENERG RES JI BioEnergy Res. PD JUN PY 2013 VL 6 IS 2 BP 841 EP 850 DI 10.1007/s12155-013-9310-y PG 10 WC Energy & Fuels; Environmental Sciences SC Energy & Fuels; Environmental Sciences & Ecology GA 138GT UT WOS:000318497700040 ER PT J AU Bansal, R Mian, MAR Mittapalli, O Michel, AP AF Bansal, Raman Mian, M. A. Rouf Mittapalli, Omprakash Michel, Andy P. TI Molecular characterization and expression analysis of soluble trehalase gene in Aphis glycines, a migratory pest of soybean SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE Aphis glycines; soybean aphid; trehalase; qPCR; migration; alate morphs ID ACYRTHOSIPHON-PISUM; HEMIPTERA APHIDIDAE; PEA APHID; INHIBITOR; MEMBRANE; MIDGUT; TREHAZOLIN AB In insects, the enzyme trehalase plays a crucial role in energy metabolism, chitin synthesis and possibly during plant-insect interactions. We have characterized a soluble trehalase gene (Tre-1) from cDNA of Aphis glycines, a serious migratory pest of soybean. The full-length cDNA of Tre-1 in A. glycines (AyTre-1) was 2550bp long with an open reading frame of 1770bp that encoded for a 589 amino acid residues protein. Sequence assessment and phylogenetic analysis of the putative protein suggested that the selected cDNA belongs to soluble trehalase group. Quantitative PCR (qPCR) analysis in different tissues and developmental stages revealed peak mRNA levels of AyTre-1 in the gut (compared with other tissues assayed) and highest expression in the second instar compared with the other developmental stages assayed. Interestingly, a significantly increased expression of AyTre-1 (1.9-fold, P<0.05) was observed in the alate morphs compared with that in apterate morphs. However, there was no significant difference in AyTre-1 expression in A. glycinesnymphs fed with resistant and susceptible plants. Expression patterns identified in this study provide a platform to investigate the role of AyTre-1 in physiological activities such as flight and feeding in A. glycines. The characterization of soluble trehalase gene may help to develop novel strategies to manage A. glycines using trehalase inhibitors and using RNA interference for knock-down of AyTre-1 expression. C1 [Bansal, Raman; Mittapalli, Omprakash; Michel, Andy P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, USDA ARS, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Michel, AP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. EM michel.70@osu.edu FU Department of Entomology, OARDC, The Ohio State University; The Ohio Soybean Council; North-Central Soybean Research Program FX We thank Lucinda Wallace for her assistance with soybean aphid colony rearing and help during manuscript writing. This work was supported through funds provided by the Department of Entomology, OARDC, The Ohio State University; The Ohio Soybean Council, and the North-Central Soybean Research Program. NR 43 TC 6 Z9 9 U1 2 U2 21 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD JUN PY 2013 VL 103 IS 3 BP 286 EP 295 DI 10.1017/S0007485312000697 PG 10 WC Entomology SC Entomology GA 137KI UT WOS:000318434300005 PM 23445549 ER PT J AU Hagler, JR Blackmer, F AF Hagler, James R. Blackmer, Felisa TI Identifying inter- and intra-guild feeding activity of an arthropod predator assemblage SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE arthropod sampling; biological control; food chain; predation; Predator gut analysis; trophic interactions ID BEMISIA-TABACI HOMOPTERA; GUT-CONTENT ANALYSIS; POLYMERASE-CHAIN-REACTION; PREY DNA; INVERTEBRATE PREDATORS; MONOCLONAL-ANTIBODIES; SPATIAL-DISTRIBUTION; SECONDARY PREDATION; MOLECULAR ANALYSIS; LYGUS-HESPERUS AB Understanding predatorprey interactions of the arthropod community in any given ecosystem is essential in pinpointing the biological control services provided by natural enemies. Hence, four prey-specific polymerase chain reaction (PCR) assays were developed to analyse the gut contents of the cotton predator community. The four targeted prey included a herbivore/pest, omnivore/pest, omnivore/beneficial, and carnivore/beneficial. First, prey retention tests were conducted to determine how long a prey item of each target species could be detected in a predator after ingestion. The assays yielded highly variable inter-assay and intra-assay prey detection efficiencies. Then, a multifaceted field study was conducted to quantify the population dynamics of the cotton predator assemblage and to assess the frequencies of predation that each predator species exhibited on the targeted prey. In total, 1794 predators, representing 17 arthropod families, were collected over two seasons using both sweep net and whole plant sampling procedures. The predator gut assays showed that there was substantial inter-guild predation occurring on the herbivore/pest, Bemisia tabaci (Gennadius); moderate intra-guild predation on the omnivore/pest, Lygus spp. (Lygus lineolaris (Palisot de Beauvois), L. hesperus Knight, and L. elisus Van Duzee) and the omnivore/beneficial, Geocoris spp. (Geocoris punctipes (Say), and G. pallens Stal); and very little intra-guild predation on the carnivore/beneficial, Collops vittatus (Say). The gut assays also revealed that DNA of the targeted pests, B. tabaci and Lygus spp., were found more frequently in insect predators than spiders; whereas there were no significant differences in predation between the predatory insects and spiders for the beneficial insects, Geocoris spp. and C. vittatus. Finally, there was a significantly higher frequency of predation events recorded for B. tabaci, Lygus spp., and Geocoris spp. in the sweep net samples. This indicates that the method of collection might influence the interpretation of the gut assay results. C1 [Hagler, James R.; Blackmer, Felisa] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Hagler, JR (reprint author), USDA ARS, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov FU USDA Risk Assessment and Mitigation Grant Program (RAMP) [ARZT-358320-G-30-505] FX We would like to thank Joel Gilley, Geoff Kimmel, Scott Machtley, Chris McNeely, Cassandra Price, Lori Stuart, Andrew Theis, and Alyssa Yamamura for their excellent technical support. The manuscript was improved by comments from Jesse de Leon and James Harwood. This work was funded, in part, by the USDA Risk Assessment and Mitigation Grant Program (RAMP # ARZT-358320-G-30-505). NR 43 TC 8 Z9 9 U1 5 U2 95 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 J9 ECOL ENTOMOL JI Ecol. Entomol. PD JUN PY 2013 VL 38 IS 3 BP 258 EP 271 DI 10.1111/een.12014 PG 14 WC Entomology SC Entomology GA 138LH UT WOS:000318509600006 ER PT J AU Grinnan, R Carter, TE Johnson, MTJ AF Grinnan, Rose Carter, Thomas E., Jr. Johnson, Marc T. J. TI The effects of drought and herbivory on plantherbivore interactions across 16 soybean genotypes in a field experiment SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Climate change; insect outbreak; plant defences; plantinsect interactions; plant stress; insect resistance; tolerance ID CLIMATE-CHANGE; INSECT RESISTANCE; RESOURCE AVAILABILITY; BEETLE RESISTANCE; PLANT GENOTYPE; UNITED-STATES; GLOBAL CHANGE; WATER-STRESS; FOOD PLANTS; METAANALYSIS AB As the Earth's climate continues to change, drought and insect population outbreaks are predicted to increase in many parts of the world. It is therefore important to understand how changes in such abiotic and biotic stressors might impact agroecosystems. The plant stress hypothesis predicts that, owing to physiological and biochemical changes, plants experiencing drought will be more susceptible to insect herbivory, which could have synergistic negative effects on plant performance. By contrast, the plant vigor hypothesis predicts that insects will preferentially feed on fast-growing vigorous plants. These hypotheses were tested in a field experiment using 16 soybean (Glycine max (L.) Merr.) genotypes to determine: (i) the combined effects of drought and herbivory on plant performance; (ii) the impact of drought on soybean resistance to herbivores; and (iii) how genetically variable phenotypic traits in soybean correlate with these responses. It was found that drought had a greater effect on soybean performance than herbivory, and drought and herbivory did not interact to impact on any measure of plant performance. Drought caused decreased insect herbivory on average, suggesting that the plant vigor hypothesis is consistent with the effects of drought stress on soybean resistance to leaf-chewing insect herbivores. This conclusion is further supported by genotypic correlations which show that plant growth rate is positively correlated with the amount of herbivory plants received. These results suggest that, although the effects of climate-associated changes in drought and herbivory will have negative effects on soybean, these potential effects are quantifiable with simple experiments and can be mitigated through continued breeding of varieties that are tolerant and resistant to these abiotic and biotic stressors. C1 [Grinnan, Rose] N Carolina State Univ, Dept Plant Biol, Raleigh, NC 27695 USA. [Carter, Thomas E., Jr.] USDA ARS, Raleigh, NC 27695 USA. [Johnson, Marc T. J.] Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. RP Johnson, MTJ (reprint author), Univ Toronto, Dept Biol, Mississauga, ON L5L 1C6, Canada. EM marc.johnson@utoronto.ca FU NC State University; USDA-ARS; NSERC Canada FX We thank the staff at the NCSU Lake Wheeler Road Field Laboratory and especially Chad Carter. This work was made possible through laboratory and field assistance from J. Bailey, W. Bolin, B. Geisler, K. Geisler, M. Gibbons, D. Gonzales, M. Grinnan, A. Hayden, E. Hersch-Green, E. Huie, H. Huie, S. Jaconis, J. Konowski, S. Kreilcamp, R. Marchin, R. Masson, C. Myburg, A. Noble, M. Novitzky, C. O'Dell, A. Parmar, J. Rabon, N. Turley and A. Wines. J. Bachelor, E. Fiscus, S. Griggs and B. Hoffman provided insight, equipment and technical assistance. B. Hoffman also provided assistance in interpreting physiological data. This work was facilitated by the USDA-ARS Soybean and Nitrogen Fixation Research Unit in Raleigh NC. We also thank NC State University (RG, MTJJ), USDA-ARS (TEC) and NSERC Canada for funding. NR 78 TC 5 Z9 5 U1 4 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 J9 ECOL ENTOMOL JI Ecol. Entomol. PD JUN PY 2013 VL 38 IS 3 BP 290 EP 302 DI 10.1111/een.12017 PG 13 WC Entomology SC Entomology GA 138LH UT WOS:000318509600009 ER PT J AU Wang, L Jiang, XF Luo, LZ Stanley, D Sappington, TW Zhang, L AF Wang, Ling Jiang, Xingfu Luo, Lizhi Stanley, David Sappington, Thomas W. Zhang, Lei TI A cadherin-like protein influences Bacillus thuringiensis Cry1Ab toxicity in the oriental armyworm, Mythimna separata SO ENVIRONMENTAL MICROBIOLOGY REPORTS LA English DT Article ID PINK-BOLLWORM; LEPIDOPTERA-NOCTUIDAE; AMINOPEPTIDASE-N; TOXIN CRY1AC; RESISTANCE; BINDING; RECEPTOR; STRAIN; GENES AB Cadherins comprise a family of calcium-dependent cell adhesion proteins that act in cellcell interactions. Cadherin-like proteins (CADs) in midguts of some insects act as receptors that bind some of the toxins produced by the Bacillus thuringiensis (Bt). We cloned a CAD gene associated with larval midguts prepared from Mythimna separata. The full-length cDNA (MsCAD1, GenBank Accession No. JF951432) is 5642bp, with an open reading frame encoding a 1757 amino acid and characteristics typical of insect CADs. Expression of MsCAD1 is predominantly in midgut tissue, with highest expression in the 3rd- to 6th-instars and lowest in newly hatched larvae. Knocking-down MsCAD1 decreased Cry1Ab susceptibility, indicated by reduced developmental time, increased larval weight and reduced larval mortality. We expressed MsCAD1 in E.coli and recovered the recombinant protein, rMsCAD1, which binds Cry1Ab toxin. Truncation analysis and binding experiments revealed that a contiguous 209-aa, located in CR11 and CR12, is the minimal Cry1Ab binding region. These results demonstrate that MsCAD1 is associated with Cry1Ab toxicity and is one of the Cry1Ab receptors in this insect. The significance of this work lies in identifying MsCAD1 as a Cry1Ab receptor, which helps understand the mechanism of Cry1Ab toxicity and of potential resistance to Bt in M.separata. C1 [Wang, Ling; Jiang, Xingfu; Luo, Lizhi; Zhang, Lei] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Stanley, David] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. [Sappington, Thomas W.] Iowa State Univ, USDA, ARS, Corn Insects & Crop Genet Res Unit,Genet Lab, Ames, IA 50011 USA. RP Jiang, XF (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM xfjiang@ippcaas.cn FU National Department of Transgenic Crops Cultivation Research Foundation [2009ZX08011-018B]; National Natural Science Foundation of China [31000850, 31071641, 30871641]; National Department of Public Benefit Research Foundation [200903051, 201303057] FX This work was funded by projects under the National Department of Transgenic Crops Cultivation Research Foundation (No. 2009ZX08011-018B), the National Natural Science Foundation of China (No. 31000850, 31071641, 30871641), and the National Department of Public Benefit Research Foundation (No. 200903051, 201303057). NR 23 TC 3 Z9 4 U1 0 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1758-2229 J9 ENV MICROBIOL REP JI Environ. Microbiol. Rep. PD JUN PY 2013 VL 5 IS 3 SI SI BP 438 EP 443 DI 10.1111/1758-2229.12036 PG 6 WC Environmental Sciences; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 133BD UT WOS:000318111500011 PM 23754724 ER PT J AU Gieraltowski, L Julian, E Pringle, J Macdonald, K Quilliam, D Marsden-Haug, N Saathoff-Huber, L Von Stein, D Kissler, B Parish, M Elder, D Howard-King, V Besser, J Sodha, S Loharikar, A Dalton, S Williams, I Behravesh, CB AF Gieraltowski, L. Julian, E. Pringle, J. Macdonald, K. Quilliam, D. Marsden-Haug, N. Saathoff-Huber, L. Von Stein, D. Kissler, B. Parish, M. Elder, D. Howard-King, V. Besser, J. Sodha, S. Loharikar, A. Dalton, S. Williams, I. Behravesh, C. Barton TI Nationwide outbreak of Salmonella Montevideo infections associated with contaminated imported black and red pepper: warehouse membership cards provide critical clues to identify the source SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Enteric bacteria; epidemiology; outbreaks; Salmonella ID TYPHIMURIUM; SEROTYPE; CANADA; SALAMI AB In November 2009, we initiated a multistate investigation of Salmonella Montevideo infections with pulsed-field gel electrophoresis pattern JIXX01.0011. We identified 272 cases in 44 states with illness onset dates ranging from 1 July 2009 to 14 April 2010. To help generate hypotheses, warehouse store membership card information was collected to identify products consumed by cases. These records identified 19 ill persons who purchased company A salami products before onset of illness. A case-control study was conducted. Ready-to-eat salami consumption was significantly associated with illness (matched odds ratio 8.5, 95% confidence interval 2.1-75.9). The outbreak strain was isolated from company A salami products from an environmental sample from one manufacturing plant, and sealed containers of black and red pepper at the facility. This outbreak illustrates the importance of using membership card information to assist in identifying suspect vehicles, the potential for spices to contaminate ready-to-eat products, and preventing raw ingredient contamination of these products. C1 [Gieraltowski, L.; Loharikar, A.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Epidem Intelligence Serv, Atlanta, GA USA. [Julian, E.; Quilliam, D.] Rhode Isl Dept Hlth, Providence, RI 02908 USA. [Pringle, J.; Besser, J.; Sodha, S.; Dalton, S.; Williams, I.; Behravesh, C. Barton] Ctr Dis Control & Prevent, Atlanta, GA USA. [Macdonald, K.; Marsden-Haug, N.] Washington State Dept Hlth, Olympia, WA USA. [Saathoff-Huber, L.] Illinois Dept Publ Hlth, Springfield, IL 62761 USA. [Von Stein, D.] Iowa Dept Hlth, De Moines, IA USA. [Kissler, B.] US Food Safety & Inspect Serv, USDA, Atlanta, GA USA. [Parish, M.; Elder, D.; Howard-King, V.] US FDA, Rockville, MD 20857 USA. RP Gieraltowski, L (reprint author), 1600 Clifton Rd NE,MS A38, Atlanta, GA 30329 USA. EM lax2@cdc.gov NR 23 TC 17 Z9 17 U1 1 U2 21 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUN PY 2013 VL 141 IS 6 BP 1244 EP 1252 DI 10.1017/S0950268812001859 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 135SS UT WOS:000318309400014 PM 23200262 ER PT J AU Niks, RE van Heyzen, S Szabo, LJ Alemu, SK AF Niks, Rients E. van Heyzen, Skye Szabo, Les J. Alemu, Sisay K. TI Host status of barley to Puccinia coronata from couch grass and P. striiformis from wheat and brome SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Agropyron; Bromus; Crown rust; Hordeum; Stripe rust; Triticum ID RUST FUNGI; STRIPE RUST; RESISTANCE; NONHOST; MORPHOLOGY; GENETICS; HORDEI; RANGE AB The pathogenicity and identity was studied of a field sample (PcE) of crown rust fungus Puccinia coronata collected in Hungary on wild couch grass (Elymus repens) and of a field sample (Psb) of stripe rust (P. striiformis) collected in the Netherlands on California brome (Bromus carinatus). We focused on the analysis of the host susceptibility of cultivated barley (Hordeum vulgare) to both pathogen samples, in direct comparison with an isolate of P. striiformis f.sp. tritici (Pst) and P. striiformis f.sp. hordei (Psh). At the seedling stage 83 % of the barley accessions were susceptible to PcE. At the adult plant stage, cultivated barley was predominantly resistant to this crown rust sample. Morphology, pathogenicity on Hordeum and Bromus and ITS DNA sequence analysis determined that PcE represents a European specimen of P. coronata f.sp. hordei. Sample Psb was pathogenic on 47 % of the cultivated barley at the seedling stage. This is intermediate between the isolates Psh (90 %) and Pst (10 %). Psb and Psh were pathogenic to grasses representing several genera. The host range of the Pst isolate was more narrow. We conclude that Psb is unlikely to represent a genotype of Psh that happened to have colonized B. carinatus, and is likely a distinct form of P. striiformis. C1 [Niks, Rients E.; van Heyzen, Skye; Alemu, Sisay K.] Wageningen Univ, Grad Sch Expt Plant Sci, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands. [Szabo, Les J.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Niks, RE (reprint author), Wageningen Univ, Grad Sch Expt Plant Sci, Lab Plant Breeding, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. EM Rients.Niks@wur.nl NR 29 TC 3 Z9 3 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUN PY 2013 VL 136 IS 2 BP 393 EP 405 DI 10.1007/s10658-013-0174-y PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 135OG UT WOS:000318297200018 ER PT J AU Tang, HZ Chen, MS Davis, J Gao, W AF Tang, Hongzhao Chen, Maosi Davis, John Gao, Wei TI Comparison of aerosol optical depth of UV-B monitoring and research program (UVMRP), AERONET and MODIS over continental united states SO FRONTIERS OF EARTH SCIENCE LA English DT Article DE aerosol optical depth (AOD); United States Department of Agriculture (USDA) UV-B Monitoring and Research Program (UVMRP); Aerosol Robotic Network (AERONET); Moderate Resolution Imaging Spectroradiameters (MODIS); validation; spatial-temporal approach ID TROPOSPHERIC AEROSOL; PARTICULATE MATTER; LAND; CLIMATOLOGY; ALGORITHMS; PRODUCTS AB The concern about the role of aerosols as to their effect in the Earth-Atmosphere system requires observation at multiple temporal and spatial scales. The Moderate Resolution Imaging Spectroradiameters (MODIS) is the main aerosol optical depth (AOD) monitoring satellite instrument, and its accuracy and uncertainty need to be validated against ground based measurements routinely. The comparison between two ground AOD measurement programs, the United States Department of Agriculture (USDA) Ultraviolet-B Monitoring and Research Program (UVMRP) and the Aerosol Robotic Network (AERONET) program, confirms the consistency between them. The intercomparison between the MODIS AOD, the AERONET AOD, and the UVMRP AOD suggests that the UVMRP AOD measurements are suited to be an alternative ground-based validation source for satellite AOD products. The experiments show that the spatial-temporal dependency between the MODIS AOD and the UVMRP AOD is positive in the sense that the MODIS AOD compare more favorably with the UVMRP AOD as the spatial and temporal intervals are increased. However, the analysis shows that the optimal spatial interval for all time windows is defined by an angular subtense of around 1A degrees to 1.25A degrees, while the optimal time window is around 423 to 483 minutes at most spatial intervals. The spatial-temporal approach around 1.25A degrees & 423 minutes shows better agreement than the prevalent strategy of 0.25A degrees & 60 minutes found in other similar investigations. C1 [Tang, Hongzhao; Chen, Maosi; Davis, John; Gao, Wei] Colorado State Univ, USDA UV B Monitoring & Res Program, Nat Resource Ecol Lab, Ft Collins, CO 80521 USA. [Gao, Wei] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80521 USA. RP Tang, HZ (reprint author), Colorado State Univ, USDA UV B Monitoring & Res Program, Nat Resource Ecol Lab, Ft Collins, CO 80521 USA. EM hongzhao.tang@colostate.edu RI Gao, Wei/O-1208-2013; Gao, Wei/C-1430-2016; Chen, Maosi/E-8230-2016 FU USDA UVB Monitoring and Research Program under USDA NIFA project [2012-34263-19736]; USDA UVB Monitoring and Research Program under USDA-NRI project [2008-35615-04666] FX This work is supported partially by USDA UVB Monitoring and Research Program under Grant No. USDA NIFA project (2012-34263-19736) and Grant No. USDA-NRI project (2008-35615-04666). NR 32 TC 1 Z9 1 U1 0 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2095-0195 J9 FRONT EARTH SCI-PRC JI Front. Earth Sci. PD JUN PY 2013 VL 7 IS 2 BP 129 EP 140 DI 10.1007/s11707-013-0376-9 PG 12 WC Geosciences, Multidisciplinary SC Geology GA 138JN UT WOS:000318504900001 ER PT J AU Nusawardani, T Kroemer, JA Choi, MY Jurenka, RA AF Nusawardani, T. Kroemer, J. A. Choi, M-Y. Jurenka, R. A. TI Identification and characterization of the pyrokinin/pheromone biosynthesis activating neuropeptide family of G protein-coupled receptors from Ostrinia nubilalis SO INSECT MOLECULAR BIOLOGY LA English DT Article DE PBAN; diapause hormone; periviscerokinins; G protein-coupled receptors; quantitative PCR ID EUROPEAN CORN-BORER; MULTIPLE SEQUENCE ALIGNMENT; HAWKMOTH MANDUCA-SEXTA; HELIOTHIS-VIRESCENS; FUNCTIONAL-CHARACTERIZATION; PYROKININ-1 RECEPTOR; EXPRESSION ANALYSIS; PBAN RECEPTOR; BOMBYX-MORI; PEPTIDES AB Insects have two closely related G protein-coupled receptors belonging to the pyrokinin/pheromone biosynthesis activating neuropeptide (pyrokinin/PBAN) family, one with the ligand PBAN or pyrokinin-2 and another with diapause hormone or pyrokinin-1 as a ligand. A related receptor is activated by products of the capa gene, periviscerokinins. Here we characterized the PBAN receptor and the diapause hormone receptor from the European corn borer, Ostrinia nubilalis. We also identified a partial sequence for the periviscerokinin receptor. Quantitative PCR of mRNA for all three receptors indicated differential expression in various life stages and tissues. All three splice variants of the PBAN receptor were identified with all variants found in pheromone gland tissue. Immunohistochemistry of V5 tags of expressed receptors indicated that all three variants and the diapause hormone receptor were expressed at similar levels in Spodoptera frugiperda 9 (Sf9) cells. However, the A- and B-variants were not active in our functional assay, which confirms studies from other moths. Functional expression of the C-variant indicated that it is has a 44nM half effective concentration for activation by PBAN. The diapause hormone receptor was activated by diapause hormone with a 150nM half effective concentration. C1 [Nusawardani, T.; Kroemer, J. A.; Jurenka, R. A.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Choi, M-Y.] ARS, USDA, Gainesville, FL USA. RP Jurenka, RA (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM rjurenka@iastate.edu OI Choi, Man-Yeon/0000-0003-0769-380X FU BARD, The United States-Israel Binational Agricultural Research and Development Fund [IS-4163-08C] FX This work was supported by research grant IS-4163-08C from BARD, The United States-Israel Binational Agricultural Research and Development Fund. NR 33 TC 15 Z9 16 U1 1 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD JUN PY 2013 VL 22 IS 3 BP 331 EP 340 DI 10.1111/imb.12025 PG 10 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA 137NU UT WOS:000318443400009 PM 23551811 ER PT J AU Wang, H Liu, G Li, CX Powell, ALT Reid, MS Zhang, Z Jiang, CZ AF Wang, Hong Liu, Gang Li, Chunxia Powell, Ann L. T. Reid, Michael S. Zhang, Zhen Jiang, Cai-Zhong TI Defence responses regulated by jasmonate and delayed senescence caused by ethylene receptor mutation contribute to the tolerance of petunia to Botrytis cinerea SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID PROGRAMMED CELL-DEATH; METHYL JASMONATE; LEAF SENESCENCE; PLANT DEFENSE; ARABIDOPSIS-THALIANA; DISEASE DEVELOPMENT; CYSTEINE PROTEASE; PATHOGEN DEFENSE; GENE-EXPRESSION; SALICYLIC-ACID AB Ethylene and jasmonate (JA) have powerful effects when plants are challenged by pathogens. The inducible promoter-regulated expression of the Arabidopsis ethylene receptor mutant ethylene-insensitive1-1 (etr1-1) causes ethylene insensitivity in petunia. To investigate the molecular mechanisms involved in transgenic petunia responses to Botrytis cinerea related to the ethylene and JA pathways, etr1-1-expressing petunia plants were inoculated with Botrytis cinerea. The induced expression of etr1-1 by a chemical inducer dexamethasone resulted in retarded senescence and reduced disease symptoms on detached leaves and flowers or intact plants. The extent of decreased disease symptoms correlated positively with etr1-1 expression. The JA pathway, independent of the ethylene pathway, activated petunia ethylene response factor (PhERF) expression and consequent defence-related gene expression. These results demonstrate that ethylene induced by biotic stress influences senescence, and that JA in combination with delayed senescence by etr1-1 expression alters tolerance to pathogens. C1 [Wang, Hong; Liu, Gang; Li, Chunxia; Zhang, Zhen] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Hong; Powell, Ann L. T.; Reid, Michael S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Jiang, Cai-Zhong] Agr Res Serv, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. RP Zhang, Z (reprint author), Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. EM zhangzh@njau.edu.cn; cjiang@ucdavis.edu OI Jiang, Cai-Zhong/0000-0002-5972-7963 FU National 863 Projects of China [2011AA100204] FX This work was supported by the National 863 Projects of China (No. 2011AA100204). NR 80 TC 8 Z9 9 U1 1 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD JUN PY 2013 VL 14 IS 5 BP 453 EP 469 DI 10.1111/mpp.12017 PG 17 WC Plant Sciences SC Plant Sciences GA 136YZ UT WOS:000318401500002 PM 23437935 ER PT J AU Franzluebbers, AJ AF Franzluebbers, Alan J. TI Editorial: Introduction to themed section-supporting ecosystem services with conservation agricultural approaches SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Editorial Material C1 ARS, USDA, Raleigh, NC 27695 USA. RP Franzluebbers, AJ (reprint author), ARS, USDA, 3218 Williams Hall,NCSU Campus Box 7619, Raleigh, NC 27695 USA. EM alan.franzluebbers@ars.usda.gov NR 5 TC 1 Z9 1 U1 0 U2 11 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD JUN PY 2013 VL 28 IS 2 BP 99 EP 101 DI 10.1017/S1742170513000021 PG 3 WC Agriculture, Multidisciplinary SC Agriculture GA 135RP UT WOS:000318306500001 ER PT J AU Sanderson, MA Archer, D Hendrickson, J Kronberg, S Liebig, M Nichols, K Schmer, M Tanaka, D Aguilar, J AF Sanderson, Matt A. Archer, David Hendrickson, John Kronberg, Scott Liebig, Mark Nichols, Kris Schmer, Marty Tanaka, Don Aguilar, Jonathan TI Diversification and ecosystem services for conservation agriculture: Outcomes from pastures and integrated crop-livestock systems SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE cropland; crop rotations; crop sequencing; ecosystem function; integrated agricultural systems; soil biology; soil carbon management ID NORTHERN GREAT-PLAINS; ARBUSCULAR MYCORRHIZAL FUNGI; SOIL CARBON DYNAMICS; PLANT DIVERSITY; CROP/LIVESTOCK SYSTEMS; COVER CROPS; PERENNIAL POLYCULTURES; SPECIES COMPOSITION; FORAGE MIXTURES; ORGANIC-MATTER AB Conservation agricultural systems rely on three principles to enhance ecosystem services: (1) minimizing soil disturbance, (2) maximizing soil surface cover and (3) stimulating biological activity. In this paper, we explore the concept of diversity and its role in maximizing ecosystem services from managed grasslands and integrated agricultural systems (i.e., integrated crop-livestock-forage systems) at the field and farm level. We also examine trade-offs that may be involved in realizing greater ecosystem services. Previous research on livestock production systems, particularly in pastureland, has shown improvements in herbage productivity and reduced weed invasion with increased forage diversity but little response in terms of animal production. Managing forage diversity in pastureland requires new tools to guide the selection and placement of plant mixtures across a farm according to site suitability and the goals of the producer. Integrated agricultural systems embrace the concept of dynamic cropping systems, which incorporates a long-term strategy of annual crop sequencing that optimizes crop and soil use options to attain production, economic and resource conservation goals by using sound ecological management principles. Integrating dynamic cropping systems with livestock production increases the complexity of management, but also creates synergies among system components that may improve resilience and sustainability while fulfilling multiple ecosystem functions. Diversified conservation agricultural systems can sustain crop and livestock production and provide additional ecosystem services such as soil C storage, efficient nutrient cycling and conservation of biodiversity. C1 [Sanderson, Matt A.; Archer, David; Hendrickson, John; Kronberg, Scott; Liebig, Mark; Nichols, Kris; Tanaka, Don; Aguilar, Jonathan] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Schmer, Marty] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Sanderson, MA (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM matt.sanderson@ars.usda.gov OI Archer, David/0000-0002-4816-7040 NR 170 TC 19 Z9 20 U1 8 U2 172 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD JUN PY 2013 VL 28 IS 2 BP 129 EP 144 DI 10.1017/S1742170512000312 PG 16 WC Agriculture, Multidisciplinary SC Agriculture GA 135RP UT WOS:000318306500004 ER PT J AU Cavigelli, MA Mirsky, SB Teasdale, JR Spargo, JT Doran, J AF Cavigelli, Michel A. Mirsky, Steven B. Teasdale, John R. Spargo, John T. Doran, John TI Organic grain cropping systems to enhance ecosystem services SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Review DE organic farming; ecosystem services; grain cropping systems; nutrient management; reduced tillage; phenological diversity of crop rotations ID CONVENTIONAL FARMING SYSTEMS; MID-ATLANTIC REGION; COVER-CROP; NO-TILLAGE; CONSERVATION TILLAGE; ECONOMIC-PERFORMANCE; WEED SUPPRESSION; SOIL QUALITY; AGRONOMIC PERFORMANCE; CARBON SEQUESTRATION AB Organic grain cropping systems can enhance a number of ecosystem services compared with conventional tilled (CT) systems. Recent results from a limited number of long-term agricultural research (LTAR) studies suggest that organic grain cropping systems can also increase several ecosystem services relative to conventional no-till (NT) cropping systems: soil C sequestration and soil N fertility (N mineralization potential) can be greater while global warming potential (GWP) can be lower in organic systems that use animal manures and cover crops compared with conventional NT systems. However, soil erosion from organic systems and nitrous oxide (N2O, a greenhouse gas) emissions from manure-based organic systems appear to be greater than from conventional NT systems, though data are limited. Also, crop yields, on average, continue to be lower and labor requirements greater in organic than in both tilled and NT conventional systems. Ecosystem services provided by organic systems may be improved by expanding crop rotations to include greater crop phenological diversity, improving nutrient management, and reducing tillage intensity and frequency. More diverse crop rotations, especially those that include perennial forages, can reduce weed pressure, economic risk, soil erosion, N2O emissions, animal manure inputs, and soil P loading, while increasing grain yield and soil fertility. Side-dressing animal manures in organic systems may increase corn nitrogen use efficiency and also minimize animal manure inputs. Management practices that reduce tillage frequency and intensity in organic systems are being developed to reduce soil erosion and labor and energy needs. On-going research promises to further augment ecosystem services provided by organic grain cropping systems. C1 [Cavigelli, Michel A.; Mirsky, Steven B.; Teasdale, John R.] ARS, USDA, Beltsville, MD 20705 USA. [Spargo, John T.] Univ Massachusetts, West Expt Stn, Amherst, MA 01003 USA. [Doran, John] ARS, USDA, Lincoln, NE 68583 USA. RP Cavigelli, MA (reprint author), ARS, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM michel.cavigelli@ars.usda.gov RI Burr, Mark/L-2722-2015 NR 126 TC 20 Z9 20 U1 7 U2 129 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD JUN PY 2013 VL 28 IS 2 BP 145 EP 159 DI 10.1017/S1742170512000439 PG 15 WC Agriculture, Multidisciplinary SC Agriculture GA 135RP UT WOS:000318306500005 ER PT J AU Franzluebbers, AJ Stuedemann, JA Seman, DH AF Franzluebbers, Alan J. Stuedemann, John A. Seman, Dwight H. TI Stocker performance and production in mixed tall fescue-bermudagrass pastures of the Southern Piedmont USA SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE botanical composition; broiler litter; cattle production; grazing pressure; organic fertilizer; stocking density ID ANIMAL PERFORMANCE; NUTRITIVE-VALUE; ENDOPHYTE; PERSISTENCE; MANAGEMENT; COASTAL; FORAGE; FERTILIZATION; QUALITY; YIELD AB Stocker performance and production from mixed cool-and warm-season perennial pastures are important determinants of agricultural sustainability that can be influenced by management. We evaluated the factorial combination of three sources of nutrient application (inorganic only, organic + inorganic combination, and organic only) and two forage utilization regimes [low grazing pressure (LGP) and high grazing pressure (HGP)] on steer stocking density and rate, performance and production during 7 years of pasture management {tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] overseeded into existing Coastal bermudagrass [Cynodon dactylon (L.) Pers.] sod} on a Typic Kanhapludult in Georgia, USA. Nutrient source had few major impacts on responses, except for lower animal performance with organic fertilization (broiler litter) than with organic + inorganic and inorganic only fertilization, especially with LGP. Seasonal changes in stocking weight and rate occurred, not only as expected due to environmental conditions and dominant forage species present, but that also counteracted expected differences imposed by grazing pressure; signaling negative feedback of HGP on forage productivity. Steer performance was greatest in spring and summer under both grazing pressures, but was significantly reduced with increasing grazing pressure in the autumn and winter due to low forage availability. Across years, steer gain ha(-1) (863 kg ha(-1)) was not different between grazing pressures, but gain ha(-1) declined with time under HGP and was stable with time under LGP. Reducing grazing pressure to a moderate level can lead to equivalent steer production as HGP, and would likely contribute to a more sustainable balance among production, socio-economic and environmental goals. These multi-year results will help cattle producers in warm, moist climates design and implement more sustainable grazing systems. C1 [Franzluebbers, Alan J.; Stuedemann, John A.; Seman, Dwight H.] ARS, USDA, Watkinsville, GA 30677 USA. RP Franzluebbers, AJ (reprint author), ARS, USDA, 1420 Expt Stn Rd, Watkinsville, GA 30677 USA. EM alan.franzluebbers@ars.usda.gov NR 30 TC 1 Z9 1 U1 1 U2 12 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD JUN PY 2013 VL 28 IS 2 BP 160 EP 172 DI 10.1017/S1742170512000105 PG 13 WC Agriculture, Multidisciplinary SC Agriculture GA 135RP UT WOS:000318306500006 ER PT J AU Simsek, H Kasi, M Ohm, JB Blonigen, M Khan, E AF Simsek, Halis Kasi, Murthy Ohm, Jae-Bom Blonigen, Mark Khan, Eakalak TI Bioavailable and biodegradable dissolved organic nitrogen in activated sludge and trickling filter wastewater treatment plants SO WATER RESEARCH LA English DT Article DE Biodegradable dissolved organic nitrogen (BDON); Bioavailable dissolved organic nitrogen (ABDON); Activated sludge; Trickling filters; Wastewater AB A study was carried out to understand the fate of biodegradable dissolved organic nitrogen (BDON) and bioavailable dissolved organic nitrogen (ABDON) along the treatment trains of a wastewater treatment facility (WWTF) equipped with an activated sludge (AS) system and a WWTF equipped with a two-stage trickling filter (TF) process. A mixed culture bacterial inoculum was used for BDON determination, while a pure cultured algal inoculum (Selenastrum capricornutum) and a combination of the bacterial and alga inocula were used for ABDON determination. Results show that BDON and ABDON varied significantly within the treatment facility and between the two facilities. From after primary clarification to final effluent, the TF facility removed 65% of BDON and 63% of ABDON while the AS facility removed 68% of BDON and 56% of ABDON. For the TF facility, BDON and ABDON were 62% and 71% of the effluent dissolved organic nitrogen (DON), while they were 26% and 47% of the effluent DON for the AS WWTF. BDON and ABDON results, which are based on incubation of samples under different inocula (bacteria only, algae only, and bacteria + algae), further showed that some portions of DON are utilizable by bacteria only or algae only while there is a portion of DON utilizable by either bacteria or algae. DON utilization was the highest when both bacteria and algae were used as a co-inoculum in the samples. This study is the first to investigate the fate of BDON and ABDON along the treatment trains of two different WWTFs. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Simsek, Halis] N Dakota State Univ, Dept Agr & Biosyst Engn, Fargo, ND 58108 USA. [Kasi, Murthy] Moore Engn Inc, West Fargo, ND 58078 USA. [Ohm, Jae-Bom] ARS, USDA, Hard Red Spring & Durum Wheat Qual Lab, Fargo, ND 58108 USA. [Blonigen, Mark] City Fargo Wastewater Treatment Plant, Fargo, ND 58102 USA. [Khan, Eakalak] N Dakota State Univ, Dept Civil Engn, Fargo, ND 58108 USA. RP Khan, E (reprint author), N Dakota State Univ, Dept Civil Engn, Dept 2470,POB 6050, Fargo, ND 58108 USA. EM eakalak.khan@ndsu.edu FU North Dakota Water Resources Research Institute FX Funding for this research was partially provided by the North Dakota Water Resources Research Institute. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the North Dakota Water Resources Research Institute. NR 16 TC 15 Z9 15 U1 6 U2 82 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD JUN 1 PY 2013 VL 47 IS 9 BP 3201 EP 3210 DI 10.1016/j.watres.2013.03.036 PG 10 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA 136QZ UT WOS:000318379800028 PM 23579086 ER PT J AU Shellie, KC King, BA AF Shellie, Krista C. King, Bradley A. TI Kaolin Particle Film and Water Deficit Influence Red Winegrape Color under High Solar Radiation in an Arid Climate SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE heat stress; radiation stress; temperature; drought; deficit irrigation ID VITIS-VINIFERA L.; LEAF GAS-EXCHANGE; CABERNET-SAUVIGNON; USE EFFICIENCY; WINE GRAPE; ANTHOCYANIN BIOSYNTHESIS; BERRY DEVELOPMENT; CROP LOAD; TEMPERATURE; IRRIGATION AB The main and interactive effects of a kaolin particle film and water deficit on vine and berry attributes of winegrape cultivars Cabernet Sauvignon and Malbec were evaluated over three growing seasons in the warm, semi-arid climate of southwestern Idaho. Berry concentrations of total anthocyanins increased with increasing severity of water deficit in both cultivars in all three study years; however, the increase was accompanied by a decrease in berry fresh weight. An equal or greater increase in total anthocyanin concentration with greater berry fresh weight was achieved in two out of three years under less severe water deficit when particle film was applied to the vine canopy. Particle film influenced stomatal conductance and leaf temperature and response differed by vine water status and cultivar, suggesting that drought response influenced the effectiveness of the film. Under the climatic conditions of this study, the combination of particle film and mild water deficit provided the greatest net increase in anthocyanin concentration. The differences observed between cultivars in response to the particle film limit the applicability of our results to cultivars with similar response to drought as Cabernet Sauvignon and Malbec. C1 [Shellie, Krista C.] ARS, Hort Crops Res Unit, USDA, Parma, ID 83660 USA. [King, Bradley A.] ARS, Northwest Irrigat & Soils Res Lab, USDA, Kimberly, ID 83341 USA. RP Shellie, KC (reprint author), ARS, Hort Crops Res Unit, USDA, 29603 U I Lane, Parma, ID 83660 USA. EM Krista.Shellie@ars.usda.gov FU ARS [5358-21000-034-00D] FX This work was conducted under ARS project no. 5358-21000-034-00D entitled Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest. The authors thank Alan Muir, Monte Shields, and Cheryl Franklin-Miller for their technical assistance and the University of Idaho Parma Research and Extension Center for the use of their field resources and materials. NR 33 TC 5 Z9 5 U1 2 U2 10 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD JUN PY 2013 VL 64 IS 2 BP 214 EP 222 DI 10.5344/ajev.2013.12067 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA AL1YX UT WOS:000338924000005 ER PT J AU Shellie, KC King, BA AF Shellie, Krista C. King, Bradley A. TI Kaolin Particle Film and Water Deficit Influence Malbec Leaf and Berry Temperature, Pigments, and Photosynthesis SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE grape; particle film; heat stress; radiation stress; solar injury ID CABERNET-SAUVIGNON; WINE GRAPE; USE EFFICIENCY; EMPIRE APPLE; ARID CLIMATE; IRRIGATION; STRESS; MERLOT; LEAVES; EXPOSURE AB The effects of kaolin particle film on traits of commercial interest in the red-skinned winegrape cultivar Malbec (Vitis vinifera L.) were evaluated over three growing seasons by measuring the surface temperatures of leaves and clusters, leaf-level assimilation, leaf and berry pigment composition, and yield of vines grown under differing severities of water deficit with and without a foliar coating of particle film. The vines were irrigated with a standard or reduced amount of water and grown under arid field conditions with high solar radiation. Particle film increased the concentration of total monomeric anthocyanins in berries each year and the ratio of anthocyanins to soluble solids at harvest in two out of three years. The particle film did not alleviate differences in anthocyanin concentration between east-and west-exposed clusters. In vines under reduced irrigation, particle film decreased the cumulative minutes that surface temperatures exceeded 30 degrees C between mid-July through harvest in east-but not west-exposed berries and in east-exposed leaves. Leaves with particle film had lower midmorning rates of assimilation, lower chlorophyll-a to chlorophyll-b ratio, and a higher ratio of chlorophylls to carotenoids than leaves without particle film. Decreased assimilation was unrelated to the magnitude of leaf reflectance of visible light. Particle film did not affect yield components or fruit maturity, indicating that net primary productivity was sufficient to ripen fruit to maturity. These results demonstrate that foliar particle film can facilitate the accumulation of anthocyanins in deficit-irrigated Malbec under warm, arid conditions with high solar radiation. C1 [Shellie, Krista C.] ARS, Hort Crops Res Unit, USDA, Parma, ID 83660 USA. [King, Bradley A.] ARS, Northwest Irrigat & Soils Res Lab, USDA, Kimberly, ID 83341 USA. RP Shellie, KC (reprint author), ARS, Hort Crops Res Unit, USDA, 29603 U I Lane, Parma, ID 83660 USA. EM Krista.Shellie@ars.usda.gov FU ARS [5358-21000-034-00D] FX This work was conducted under ARS project no. 5358-21000-034-00D entitled Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest. The authors thank Alan Muir, Monte Shields, and Cheryl Franklin-Miller for their technical assistance and the University of Idaho Parma Research and Extension Center for the use of their field resources and materials. NR 23 TC 11 Z9 11 U1 1 U2 6 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD JUN PY 2013 VL 64 IS 2 BP 223 EP 230 DI 10.5344/ajev.2012.12115 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA AL1YX UT WOS:000338924000006 ER PT J AU Zdunic, G Preece, JE Dangl, GS Koehmstedt, A Mucalo, A Maletic, E Pejic, I AF Zdunic, Goran Preece, John E. Dangl, Gerald S. Koehmstedt, Anne Mucalo, Ana Maletic, Edi Pejic, Ivan TI Genetic Characterization of Grapevine Cultivars Collected throughout the Dalmatian Region SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE native grapevines; microsatellite markers; genetic diversity; Vitis vinifera ID VITIS-VINIFERA L.; MOLECULAR ANALYSIS; PLAVAC MALI; MICROSATELLITE; CROATIA; IDENTIFICATION; ZINFANDEL; VARIETIES; GERMPLASM; PARENTAGE AB Eleven SSR loci were used to identify 76 accessions of presumed native Dalmatian grapevine cultivars (Vitis vinifera L.). Plant material was collected throughout the Dalmatian region and used to reestablish a grape germplasm collection in Dalmatia, the Adriatic region of Croatia. The 76 accessions had 63 unique genotypes, of which 35 are published here for the first time. Twelve synonyms were found within the Dalmatian cultivars and 11 Dalmatian cultivars matched previously published genotypes, mainly from neighboring countries, suggesting historic exchange of grapevine cultivars. Several cultivars with similar names had different SSR profiles, making them homonyms. Genetic distance analysis revealed five groups of cultivars and confirmed several distinct grape gene pools in Croatia. C1 [Zdunic, Goran; Mucalo, Ana] Inst Adriat Crops & Karst Reclamat, Split 21000, Croatia. [Preece, John E.; Koehmstedt, Anne] USDA ARS, Natl Clonal Germplasm Repository, Washington, DC 20250 USA. [Dangl, Gerald S.] Univ Calif Davis, Fdn Plant Serv, Davis, CA 95616 USA. [Maletic, Edi; Pejic, Ivan] Univ Zagreb, Fac Agr, Zagreb 10000, Croatia. RP Zdunic, G (reprint author), Inst Adriat Crops & Karst Reclamat, Put Duilova 11, Split 21000, Croatia. EM gzdunic@krs.hr FU Fulbright program FX Research was conducted at the National Clonal Germplasm Repository USDA-ARS, Davis. G. Zdunic acknowledges financial support for his research from the Fulbright program. The authors thank Mary Lou Mendum for her work editing the manuscript before submission. NR 31 TC 7 Z9 7 U1 0 U2 8 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD JUN PY 2013 VL 64 IS 2 BP 285 EP 290 DI 10.5344/ajev.2012.12085 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA AL1YX UT WOS:000338924000014 ER PT J AU Simon, R Spooner, DM AF Simon, Reinhard Spooner, David M. TI A NEW R PACKAGE, EXSIC, TO ASSIST TAXONOMISTS IN CREATING INDICES SO APPLICATIONS IN PLANT SCIENCES LA English DT Article DE exsic; exsiccatae; R; reproducible research; software AB Premise of the study: Taxonomists manage large amounts of specimen data. This is usually initiated in spreadsheets and then converted for publication into locality lists and indices to associate collectors and collector numbers from herbarium sheets to identifications (exsiccatae). This conversion process is mostly done by hand and is time-consuming, cumbersome, and error-prone. Methods and Results: We constructed a tool, 'exsic,' based on the statistical software R. The exsic function is part of the R package 'exsic' and produces specimen citations and exsiccatae conforming to four related formats. Conclusions: The tool increases speed, efficiency, and accuracy to convert raw spreadsheet tables to publication-ready content. C1 [Simon, Reinhard] Int Potato Ctr, Integrated IT & Computat Res Unit, Lima, Peru. [Spooner, David M.] Univ Wisconsin, Vegetable Crops Res Unit, USDA ARS, Madison, WI 53706 USA. RP Simon, R (reprint author), Int Potato Ctr, Integrated IT & Computat Res Unit, Ave Molina 1895, Lima, Peru. EM r.simon@cgiar.org OI Simon, Reinhard/0000-0002-4608-9077 FU National Science Foundation [DEB 0316614] FX This research was supported by the National Science Foundation (DEB 0316614). The authors thank two anonymous reviewers for their feedback on earlier versions, which greatly helped to improve the scope and usability of the tool. NR 7 TC 1 Z9 1 U1 0 U2 0 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 2168-0450 J9 APPL PLANT SCI JI Appl. Plant Sci. PD JUN PY 2013 VL 1 IS 6 AR 1300024 DI 10.3732/apps.1300024 PG 4 WC Plant Sciences SC Plant Sciences GA AQ1QR UT WOS:000342556600010 ER PT J AU Baranowski, T AF Baranowski, Tom TI Games and Childhood Obesity SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material C1 Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Pediat Behav Nutr & Phys Act,Dept Pediat,Baylor C, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Pediat Behav Nutr & Phys Act,Dept Pediat,Baylor C, Houston, TX 77030 USA. EM tbaranow@bcm.tmc.edu NR 36 TC 2 Z9 2 U1 1 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2013 VL 2 IS 3 SI SI BP 113 EP 115 DI 10.1089/g4h.2013.1502 PG 3 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA V40YY UT WOS:000209515100001 PM 26196722 ER PT J AU Lu, AS Kharrazi, H Gharghabi, F Thompson, D AF Lu, Amy Shirong Kharrazi, Hadi Gharghabi, Fardad Thompson, Debbe TI A Systematic Review of Health Videogames on Childhood Obesity Prevention and Intervention SO GAMES FOR HEALTH JOURNAL LA English DT Review AB Childhood obesity is a global epidemic. Health videogames are an emerging intervention strategy to combat childhood obesity. This systematic review examined published research on the effect of health videogames on childhood obesity. Fourteen articles examining 28 health videogames published between 2005 and 2013 in English were selected from 2433 articles identified through five major search engines. Results indicated that academic interest in using health videogames for childhood obesity prevention has increased during this time. Most games were commercially available. Most studies were of short duration. Diverse player and game play patterns have been identified. Most studies involved players of both genders with slightly more boys. The majority of players were non-white. Most studies had the players play the games at home, whereas some extended the play setting to school and sports/recreational facilities. Most of the games were commercially available. Positive outcomes related to obesity were observed in about 40 percent of the studies, all of which targeted overweight or obese participants. C1 [Lu, Amy Shirong] Northwestern Univ, Dept Commun Studies, Sch Commun, Evanston, IL 60208 USA. [Kharrazi, Hadi] Johns Hopkins Univ, Dept Hlth Policy & Management, Bloomberg Sch Publ Hlth, Baltimore, MD 21218 USA. [Gharghabi, Fardad] Indiana Univ, Sch Informat, Indianapolis, IN 46204 USA. [Thompson, Debbe] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Lu, AS (reprint author), Northwestern Univ, Dept Commun Studies, Sch Commun, 2240 Campus Dr, Evanston, IL 60208 USA. EM amylu@northwestern.edu RI Kharrazi, Hadi/Q-1725-2015; OI Kharrazi, Hadi/0000-0003-1481-4323; Lu, Amy Shirong/0000-0002-8230-9049 FU NCI NIH HHS [R21 CA158917] NR 57 TC 28 Z9 28 U1 1 U2 3 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2013 VL 2 IS 3 SI SI BP 131 EP 141 DI 10.1089/g4h.2013.0025 PG 11 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA V40YY UT WOS:000209515100005 PM 24353906 ER PT J AU Mellecker, R Lyons, EJ Baranowski, T AF Mellecker, Robin Lyons, Elizabeth J. Baranowski, Tom TI Disentangling Fun and Enjoyment in Exergames Using an Expanded Design, Play, Experience Framework: A Narrative Review SO GAMES FOR HEALTH JOURNAL LA English DT Article AB With exergames (as with physical activity in general), more intense and longer-duration game play should accrue more health benefits. Exergames, however, appear to be played for relatively short durations, often at medium or lower intensities. Ostensibly games are played for fun or enjoyment. Enhancing the fun or enjoyment experienced during exergame play should enhance the intensity and duration of physical activity, and thereby the health benefits. Research, reviewed herein, indicates fun and/or enjoyment in games are inherently laden with psychosocial, physiological, and embodiment substrates. Physical activity may also have separate or closely related psychosocial, physiological, and embodiment enjoyment substrates. Research is needed to integrate these levels of experience and to identify the game mechanics that enhance, and even maximize, the fun or enjoyment experienced in exergames, to thereby increase the health benefit. C1 [Mellecker, Robin] Univ Hong Kong, Inst Human Performance, Pokfulam, Hong Kong, Peoples R China. [Lyons, Elizabeth J.] Univ Texas Med Branch, Inst Translat Sci, Galveston, TX 77555 USA. [Baranowski, Tom] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Lyons, EJ (reprint author), Univ Texas Med Branch, Inst Translat Sci, 301 Univ Blvd, Galveston, TX 77555 USA. EM ellyons@utmb.edu OI Baranowski, Tom/0000-0002-0653-2222; Lyons, Elizabeth/0000-0003-1695-2236 NR 78 TC 20 Z9 21 U1 1 U2 1 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2013 VL 2 IS 3 SI SI BP 142 EP 149 DI 10.1089/g4h.2013.0022 PG 8 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA V40YY UT WOS:000209515100006 ER PT J AU Simons, M Baranowski, J Thompson, D Buday, R Abdelsamad, D Baranowski, T AF Simons, Monique Baranowski, Janice Thompson, Debbe Buday, Richard Abdelsamad, Dina Baranowski, Tom TI Child Goal Setting of Dietary and Physical Activity in a Serious Videogame SO GAMES FOR HEALTH JOURNAL LA English DT Article AB Objective: To inform child obesity prevention programs, the current article identified what children thought were the most important goals, values, and perceived barriers related to healthy eating and physical activity (PA) within a serious videogame for health, "Escape from Diab'' (Archimage Inc., Houston, TX). Subjects and Methods: One hundred three children, 10-12 years of age, played "Escape from Diab.'' During game play the children were presented with a menu of goals, values, and barriers from which they selected the ones most important to them. The children's selections were transmitted to a central server and stored in a database. Frequencies were calculated and reported. Results: The most important diet-related values and reasons for children were getting good grades and being healthy and fit. The most often reported barrier for fruit intake was that it does not fill you up, and for vegetable intake it was that availability at home was limited. Also, limited availability of bottled water at home was an often chosen barrier. PA-related important values and reasons were not missing school and having energy to do homework. Children preferred to limit sedentary activities for only 30 minutes rather than for 60 minutes. The most frequently mentioned barrier for reducing inactivity was "feeling too tired to do anything else.'' Conclusions: These findings provide important input for future obesity prevention videogames attempting to motivate children to set healthy diet and PA goals. C1 [Simons, Monique] Vrije Univ Amsterdam, Dept Hlth Sci, Fac Earth & Life Sci, NL-1081 HV Amsterdam, Netherlands. [Simons, Monique] Vrije Univ Amsterdam, EMGO Inst Hlth & Care Res, Fac Earth & Life Sci, NL-1081 HV Amsterdam, Netherlands. [Simons, Monique] Vrije Univ Amsterdam Med Ctr, Body Work, Res Ctr Phys Act, Work & Hlth,TNO VU VUmc, Amsterdam, Netherlands. [Simons, Monique] TNO, Expertise Ctr Life Style, Leiden, Netherlands. [Baranowski, Janice; Thompson, Debbe; Abdelsamad, Dina; Baranowski, Tom] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. [Buday, Richard] Archimage Inc, Houston, TX USA. RP Simons, M (reprint author), Vrije Univ Amsterdam, Dept Hlth Sci, Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands. EM m.simons@vu.nl NR 48 TC 9 Z9 9 U1 0 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD JUN PY 2013 VL 2 IS 3 SI SI BP 150 EP 157 DI 10.1089/g4h.2013.0009 PG 8 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA V40YY UT WOS:000209515100007 PM 26196727 ER PT J AU Liu, ZS Knetzer, DA AF Liu, Zengshe Knetzer, Daniel A. TI Catalyzed ring-opening polymerization of epoxidized soybean oil by hydrated and anhydrous fluoroantimonic acids SO GREEN MATERIALS LA English DT Article DE epoxidized soybean oil; ring-opening polymerization; super acids AB Ring-opening polymerization of epoxidized soybean oil (ESO) catalyzed by a superacid, fluoroantimonic acid hexahydrate (HSbF6 center dot 6H(2)O) and the anhydrous form (HSbF6) in ethyl acetate was conducted in an effort to develop useful biodegradable polymers. The resulting polymerized ESO (SA-RPESO and SAA-RPESO) was characterized using infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis (TGA), H-1-NMR, C-13-NMR, solid-state C-13-NMR and gel permeation chromatography (GPC). The results indicated that ESO was effectively polymerized by fluoroantimonic acid and formed polymers with relatively high cross-link density. Glass transition temperatures of these polymers ranged from -13 degrees C to -21 degrees C. TGA results showed the SA-RPESO and SAA-RPESO polymers were thermally stable at temperatures up to 200 degrees C. Decomposition of the polymers was found to occur at temperatures greater than 350 degrees C. GPC results indicated the extracted soluble substances from SA-RPESO polymers were oligomers of ESO. These soybean oil-based polymers will be explored for application in the personal and health care areas. C1 [Liu, Zengshe; Knetzer, Daniel A.] ARS, Biooils Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Liu, ZS (reprint author), ARS, Biooils Res, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM kevin.liu@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 1 PU ICE PUBLISHING PI WESTMINISTER PA INST CIVIL ENGINEERS, 1 GREAT GEORGE ST, WESTMINISTER SW 1P 3AA, ENGLAND SN 2049-1220 EI 2049-1239 J9 GREEN MATER JI Green Mater. PD JUN PY 2013 VL 1 IS 2 BP 87 EP 95 DI 10.1680/gmat.12.00020 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Materials Science, Multidisciplinary; Polymer Science SC Science & Technology - Other Topics; Materials Science; Polymer Science GA V44ZE UT WOS:000209786100004 ER PT J AU Rossman, AY Seifert, KA Samuels, GJ Minnis, AM Schroers, HJ Lombard, L Crous, PW Poldmaa, K Cannon, PF Summerbell, RC Geiser, DM Zhuang, WY Hirooka, Y Herrera, C Salgado-Salazar, C Chaverri, P AF Rossman, Amy Y. Seifert, Keith A. Samuels, Gary J. Minnis, Andrew M. Schroers, Hans-Josef Lombard, Lorenzo Crous, Pedro W. Poldmaa, Kadri Cannon, Paul F. Summerbell, Richard C. Geiser, David M. Zhuang, Wen-ying Hirooka, Yuuri Herrera, Cesar Salgado-Salazar, Catalina Chaverri, Priscila TI Genera in Bionectriaceae, Hypocreaceae, and Nectriaceae (Hypocreales) proposed for acceptance or rejection SO IMA FUNGUS LA English DT Article DE Anamorph-typified genera; Article 59; New combinations; Nomenclature; Teleomorph-typified genera AB With the recent changes concerning pleomorphic fungi in the new International Code of Nomenclature for algae, fungi, and plants (ICN), it is necessary to propose the acceptance or protection of sexual morph-typified or asexual morph-typified generic names that do not have priority, or to propose the rejection or suppression(1) of competing names. In addition, sexual morph-typified generic names, where widely used, must be proposed for rejection or suppression in favour of asexual morph-typified names that have priority, or the latter must be proposed for conservation or protection. Some pragmatic criteria used for deciding the acceptance or rejection of generic names include: the number of name changes required when one generic name is used over another, the clarity of the generic concept, their relative frequencies of use in the scientific literature, and a vote of interested mycologists. Here, twelve widely used generic names in three families of Hypocreales are proposed for acceptance, either by conservation or protection, despite their lack of priority of publication, or because they are widely used asexual morph-typified names. Each pair of generic names is evaluated, with a recommendation as to the generic name to be used, and safeguarded, either through conservation or protection. Four generic names typified by a species with a sexual morph as type that are younger than competing generic names typified by a species with an asexual morph type, are proposed for use. Eight older generic names typified by species with an asexual morph as type are proposed for use over younger competing generic names typified by a species with a sexual morph as type. Within Bionectriaceae, Clonostachys is recommended over Bionectria; in Hypocreaceae, Hypomyces is recommended over Cladobotryum, Sphaerostilbella over Gliocladium, and Trichoderma over Hypocrea; and in Nectriaceae, Actinostilbe is recommended over Lanatonectria, Cylindrocladiella over Nectricladiella, Fusarium over Gibberella, Gliocephalotrichum over Leuconectria, Gliocladiopsis over Glionectria, Nalanthamala over Rubrinectria, Nectria over Tubercularia, and Neonectria over Cylindrocarpon. C1 [Rossman, Amy Y.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Seifert, Keith A.] Agr & Agri Food Canada, Eastern Cereal & Oilseed Res Ctr, Biodivers Mycol, Ottawa, ON K1A 0C6, Canada. [Minnis, Andrew M.] US Forest Serv, Ctr Forest Mycol Res, Northern Res Stn, USDA, Madison, WI 53726 USA. [Schroers, Hans-Josef] Agr Inst Slovenia, Ljubljana 1000, Slovenia. [Lombard, Lorenzo; Crous, Pedro W.] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. [Poldmaa, Kadri] Univ Tartu, Inst Ecol & Earth Sci, EE-51014 Tartu, Estonia. [Poldmaa, Kadri] Univ Tartu, Nat Hist Museum, EE-51014 Tartu, Estonia. [Cannon, Paul F.] Royal Bot Gardens, Jodrell Lab, Richmond TW9 3AB, Surrey, England. [Summerbell, Richard C.] Sporometrics Inc, Toronto, ON M6K 1Y9, Canada. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Buckhout Lab 121, University Pk, PA 16802 USA. [Zhuang, Wen-ying] Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China. [Hirooka, Yuuri] Forestry & Forest Prod Res Inst, Dept Forest Microbiol, Tsukuba, Ibaraki 3058687, Japan. [Herrera, Cesar; Salgado-Salazar, Catalina; Chaverri, Priscila] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. RP Rossman, AY (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM Amy.Rossman@ars.usda.gov RI Lombard, Lorenzo/B-2042-2010; Crous, Pedro/H-1489-2012 OI Lombard, Lorenzo/0000-0002-5942-5375; Crous, Pedro/0000-0001-9085-8825 NR 66 TC 40 Z9 46 U1 1 U2 14 PU INT MYCOLOGICAL ASSOC PI BERKELEY PA C/O J TAYLOR, DEPT PLANT & MICROBIAL BIOLOGY, BERKELEY, CA 94720 USA SN 2210-6340 EI 2210-6359 J9 IMA FUNGUS JI IMA Fungus PD JUN PY 2013 VL 4 IS 1 BP 41 EP 51 DI 10.5598/imafungus.2013.04.01.05 PG 11 WC Mycology SC Mycology GA V46UE UT WOS:000209908300025 PM 23898411 ER PT J AU Cushman, JC Kang, SH Tillett, RL Yobi, A Sharp, RE Schlauch, KA Oliver, MJ AF Cushman, John C. Kang, Sangho Tillett, Richard L. Yobi, Abou Sharp, Robert E. Schlauch, Karen A. Oliver, Melvin J. TI Exploring Desiccation Tolerance Using Comparative Integrative 'Omic Analyses in Resurrection Plants. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Cushman, John C.; Kang, Sangho; Tillett, Richard L.; Schlauch, Karen A.] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA. [Yobi, Abou; Oliver, Melvin J.] Univ Missouri, Plant Genet Res Unit, USDA ARS MWA, Columbia, MO 65211 USA. [Sharp, Robert E.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM jcushman@unr.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-39 BP S26 EP S26 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600069 ER PT J AU Hand, C Reed, BM AF Hand, Charles Reed, Barbara M. TI Minor Nutrients are Critical for the Improved Growth of Corylus avellana Shoot Cultures SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Hand, Charles] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2043 BP S67 EP S67 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600167 ER PT J AU Kamo, K AF Kamo, K. TI Optimized Conditions for Biolistic-mediated Transformation of Lilium longiflorum 'Nellie White' SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Kamo, K.] USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. EM Kathryn.Kamo@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2054 BP S71 EP S71 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600178 ER PT J AU Mccammon, SL AF McCammon, Sally L. TI Regulation of the Products of Biotechnology in the United States. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [McCammon, Sally L.] Anim & Hlth Inspect Serv, USDA, Biotechnol Regulatory Serv, Riverdale, MD 20737 USA. EM Sally.L.McCammon@aphis.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-28 BP S23 EP S23 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600058 ER PT J AU Neugebauer, K Bruce, M Glasscock, J Fellers, J AF Neugebauer, K. Bruce, M. Glasscock, J. Fellers, J. TI Gene Expression Differences in Wheat Induced by Six Puccinia triticina Races SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Neugebauer, K.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Bruce, M.; Fellers, J.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Glasscock, J.] Cofactor Genom, St Louis, MO 63103 USA. EM kerrin@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2037 BP S65 EP S65 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600161 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI Increased Mesos Components (CaCl2, MgSO4 and KH2PO4) Improve Growth and Quality of Micropropagated Red Raspberries SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Poothong, Sukalya] Oregon State Univ, Dept Hort, ALS 4017, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM sukalya_p@hotmail.com; Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-1005 BP S35 EP S35 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600088 ER PT J AU Rupp, JL Cruz, L Fellers, JP Trick, HN AF Rupp, Jessica L. Cruz, Luisa Fellers, John P. Trick, Harold N. TI RNAi Mediated Viral Resistance in Transgenic Wheat SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica L.; Cruz, Luisa; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Cruz, Luisa] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Fellers, John P.] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM jessicarupp@rocketmail.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2031 BP S62 EP S62 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600155 ER PT J AU Wada, S Niedz, RP Reed, BM AF Wada, Sugae Niedz, Randall P. Reed, Barbara M. TI Total Medium Nitrogen and Ammonium to Nitrate Ratio Vary for Optimal Growth of Four Pyrus Species In Vitro SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Niedz, Randall P.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2042 BP S66 EP S67 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600166 ER PT J AU Wang, Y Pijut, PM AF Wang, Y. Pijut, P. M. TI Improvement of Agrobacterium-mediated Transformation of Mature Black Cherry (Prunus serotina Ehrh.) and Rooting of Transgenic Shoots SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Wang, Y.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, P. M.] HTIRC, USDA Forest Serv, Northern Res Stn, W Lafayette, IN 47907 USA. EM wang4@purdue.edu; ppijut@purdue.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 EI 1543-706X J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUN PY 2013 VL 49 SU 1 MA P-2035 BP S64 EP S64 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA V41LL UT WOS:000209547600159 ER PT J AU Huang, YB Sui, RX Thomson, SJ Fisher, DK AF Huang, Yanbo Sui, Ruixiu Thomson, Steven J. Fisher, Daniel K. TI Estimation of cotton yield with varied irrigation and nitrogen treatments using aerial multispectral imagery SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE remote sensing; multispectral imagery; cotton; yield; nitrogen; irrigation; soil properties ID QUICKBIRD SATELLITE IMAGERY; GRAIN-SORGHUM YIELD; INDEXES; STRESS AB Cotton yield varies spatially within a field. The variability can be caused by various production inputs such as soil properties, water management, and fertilizer application. Airborne multispectral imaging is capable of providing data and information to study effects of the inputs on yield qualitatively and quantitatively in a timely and cost-effective fashion. A 10-ha cotton field with irrigation and non-irrigation 2x2 blocks was used in this study. Six nitrogen application treatments were randomized with two replications within each block. As plant canopy was closed, airborne multispectral images of the field were acquired using a 3-CCD MS4100 camera. The images were processed to generate various vegetation indices. The vegetation indices were evaluated for the best performance to characterize yield. The effect of irrigation on vegetation indices was significant. Models for yield estimation were developed and verified by comparing the estimated and actual yields. Results indicated that ratio of vegetation index (RVI) had a close relationship with yield (R-2=0.47). Better yield estimation could be obtained using a model with RVI and soil electrical conductivity (EC) measurements of the field as explanatory variables (R-2=0.53). This research demonstrates the capability of aerial multispectral remote sensing in estimating cotton yield variation and considering soil properties and nitrogen. C1 [Huang, Yanbo; Sui, Ruixiu; Thomson, Steven J.; Fisher, Daniel K.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Huang, YB (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM Yanbo.Huang@ars.usda.gov; Ruixiu.Sui@ars.usda.gov; Steve.Thomson@ars.usda.gov; Daniel.Fisher@ars.usda.gov NR 8 TC 4 Z9 4 U1 2 U2 21 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD JUN PY 2013 VL 6 IS 2 BP 37 EP 41 DI 10.3965/j.ijabe.20130602.005 PG 5 WC Agricultural Engineering SC Agriculture GA AK2RL UT WOS:000338267100005 ER PT J AU Yoon, SC Windham, WR Ladely, S Heitschmidt, GW Lawrence, KC Park, B Narang, N Cray, WC AF Yoon, Seung Chul Windham, William R. Ladely, Scott Heitschmidt, Gerald W. Lawrence, Kurt C. Park, Bosoon Narang, Neelam Cray, William C. TI Differentiation of big-six non-O157 Shiga-toxin producing Escherichia coli (STEC) on spread plates of mixed cultures using hyperspectral imaging SO JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION LA English DT Article DE Pathogen detection; Hyperspectral imaging; Shiga-toxin producing Escherichia coli (STEC); Non-O157 serogroups; Foodborne pathogen; Spread plates; Mixed cultures AB There have been considerable recent advances in the technology for rapidly detecting foodborne pathogens. However, a traditional culture method is still the "gold standard'' for presumptive-positive pathogen screening although it is labor-intensive, ineffective in testing large amount of food samples, and cannot completely prevent unwanted background microflora from growing together with target microorganisms on agar media. We have developed multivariate classification models based on visible and near-infrared hyperspectral imaging for rapid presumptive-positive screening of six representative non-O157 Shigatoxin producing Escherichia coli (STEC) serogroups (O26, O45, O103, O111, O121, and O145) on agar plates of pure and mixed cultures. The classification models were developed with spread plates of pure cultures. In this study, we evaluated the performance of the classification models with independent validation samples of mixed cultures that were not used during training and found the best classification model for differentiating non-O157 STEC colonies on spread plates of mixed cultures. A validation protocol appropriate to hyperspectral imaging of mixed cultures was developed. An additional independent validation set of 12 spread plates with pure cultures was used as positive controls to help the validation process with the mixed cultures and to affirm the model performance. One imaging experiment with colonies obtained from two serial dilutions was performed. A total of six agar plates of mixed cultures were prepared, where O45, O111 and O121 serogroups that were relatively easy to differentiate were inoculated into all six plates and then each of O26, O103 and O145 serogroups was added into the mixture of the three common bacterial cultures. The number of mixed colonies grown after 24-h incubation was 331 and the number of pixels associated with the grown colonies was 16,379. The best model found from this validation study was based on pre-processing with standard normal variate and detrending, first derivative, spectral smoothing, and k-nearest neighbor classification (kNN, k = 3) of scores in the principal component subspace spanned by 12 principal components. The results showed 95 % overall detection accuracy at pixel level and 97 % at colony level. The developed model was proven to be still valid even for the independent validation samples although the size of a validation set was small and only one experiment was performed. This study was an important first step in validating and updating multivariate classification models for rapid screening of ground beef samples contaminated by non-O157 STEC pathogens using hyperspectral imaging. C1 [Yoon, Seung Chul; Windham, William R.; Heitschmidt, Gerald W.; Lawrence, Kurt C.; Park, Bosoon] ARS, USDA, Richard Russell Res Ctr, Athens, GA 30605 USA. [Ladely, Scott; Narang, Neelam; Cray, William C.] USDA, FSIS, Outbreaks Eastern Lab, Athens, GA 30605 USA. RP Yoon, SC (reprint author), ARS, USDA, Richard Russell Res Ctr, Athens, GA 30605 USA. EM seungchul.yoon@ars.usda.gov FU National Institute for Hometown Security FX The authors would like to thank Jerrie Barnett and Peggy Feldner in Quality and Safety Assessment Research Unit in Athens for their assistant for this research. This project was supported in part by a grant from National Institute for Hometown Security. NR 20 TC 14 Z9 14 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1932-7587 EI 2193-4134 J9 J FOOD MEAS CHARACT JI J. Food Meas. Charact. PD JUN PY 2013 VL 7 IS 2 BP 47 EP 59 DI 10.1007/s11694-013-9137-4 PG 13 WC Food Science & Technology SC Food Science & Technology GA V41BG UT WOS:000209521100001 ER PT J AU Chatfield, J Milleson, M Stoddard, R Galloway, R AF Chatfield, Jenifer Milleson, Michael Stoddard, Robyn Galloway, Renee TI SEROSURVEY OF LEPTOSPIROSIS IN FERAL HOGS (SUS SCROFA) IN FLORIDA SO JOURNAL OF ZOO AND WILDLIFE MEDICINE LA English DT Article DE feral hog; Florida; leptospirosis; Sus scrofa; zoonosis ID EUROPEAN WILD HOGS; SEROLOGICAL SURVEY; POPULATION; SPP.; PREVALENCE; ANTIBODIES; CARNIVORES; PATHOGENS; EXPOSURE; BOARS AB Leptospira is a global pathogen of emerging public health importance in both developing and industrialized nations and can infect almost all mammalian species, including humans. As suburbanization and the popularity of outdoor recreational activities increases, so do human-wildlife and companion animal-wildlife interfaces. Florida offers a tropical climate favorable for outdoor activities and a semirural landscape that sustains an abundant feral hog population. Because no survey of leptospirosis in feral hogs (Sus scrofa) in Florida has been published to our knowledge, we sought to establish preliminary seroprevalence of leptospirosis exposure in feral hogs in Florida. Blood samples were collected opportunistically from 158 male and 166 female feral hogs taken at managed hunts and by permitted trappers in the northern, central, and southern regions of Florida. Samples were then analyzed using the microscopic agglutination test (MAT) for antibody titers to 20 Leptospira serovars representing 17 serogroups. A titer of >1:100 was considered positive; 33% (107/324 total samples) were positive to at least one serovar, and 46% of those were positive to multiple serovars. Antibodies to L. interrogans serovar Bratislava strain Jez Bratislava (serogroup Australis) was the most common, with 18% (58/324) testing positive for antibodies. These initial data indicate that there is a significant possibility of feral hogs having a larger role in the complex etiology of leptospirosis in Florida than historically estimated and that further investigation is warranted. C1 [Chatfield, Jenifer] Florida Dept Hlth, Zoonot & Vector Borne Dis Program, Tallahassee, FL 32399 USA. [Milleson, Michael] USDA, Natl Wildlife Dis Surveillance & Emergency Respon, Gainesville, FL 32641 USA. [Stoddard, Robyn; Galloway, Renee] Ctr Dis Control & Prevent, Bacterial Special Pathogens Branch, Atlanta, GA 30333 USA. RP Chatfield, J (reprint author), 4J Conservat Ctr, 38316 Mickler Rd, Dade City, FL 33523 USA. EM jen_chatfield@hotmail.com NR 24 TC 3 Z9 3 U1 1 U2 15 PU AMER ASSOC ZOO VETERINARIANS PI YULEE PA 581705 WHITE OAK ROAD, YULEE, FL 32097 USA SN 1042-7260 EI 1937-2825 J9 J ZOO WILDLIFE MED JI J. Zoo Wildl. Med. PD JUN PY 2013 VL 44 IS 2 BP 404 EP 407 DI 10.1638/2012-0258R2.1 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA AM1PS UT WOS:000339620500020 PM 23805559 ER EF